LOCUS       DATMSU010000001         8032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_151632,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000001 DATMSU010000000
VERSION     DATMSU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151632"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VN034_00005"
     CDS             <1..282
                     /locus_tag="VN034_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HWV59007.1"
                     /translation="KRARHDTRAWQVKRRERTRQLIELGGLIAKTDLVELTGDDRAVI
                     LGLLVEAAATLRGKARDQQLLLWRRRGRRAFANGDLQPPATTIWRTGDR"
     gene            272..439
                     /locus_tag="VN034_00010"
     CDS             272..439
                     /locus_tag="VN034_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6771 family protein"
                     /protein_id="HWV59008.1"
                     /translation="MTDKTTAIILQTVERAPQWVRRDLCSTDQAARTQAEETLAAMIA
                     DALNKTDEPPA"
     gene            complement(444..884)
                     /locus_tag="VN034_00015"
     CDS             complement(444..884)
                     /locus_tag="VN034_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWV59009.1"
                     /translation="MEDSETLVTLTADIVAAHVSNNSVAISDIPLVIRSVHEALAGLG
                     PKAEPEPEPQQPAVSVRASVKPDYILCLEDGKKLKMLRRHLMTNFGMTPDDYRAKWNL
                     PKDYPMVAPNYAEKRRELALQIGLGRKGSGGGRKPAARGRAKAK"
     gene            complement(913..1038)
                     /locus_tag="VN034_00020"
                     /pseudo
     CDS             complement(913..1038)
                     /locus_tag="VN034_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
     gene            complement(1368..1553)
                     /locus_tag="VN034_00025"
     CDS             complement(1368..1553)
                     /locus_tag="VN034_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59010.1"
                     /translation="MATIEMCAESLKDLICGTEKTRCRRSNNPRLAEDLHKPVDRRRV
                     AAAQTAAPIFILKKASD"
     gene            complement(1893..2321)
                     /locus_tag="VN034_00030"
     CDS             complement(1893..2321)
                     /locus_tag="VN034_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59011.1"
                     /translation="MILVAKDLADLGQGCAGTEKLGRETVAENMRTAMGDAADPGSLE
                     RSAGNIPDAAGRREPRMRRVGLQEELAVIRCRTTALKISSNGRSDVGRQRHDRRLPSL
                     ALNAELADAPVDVVEREGRDFLCPQSKPGEQQQDRVVAAP"
     gene            2399..3325
                     /locus_tag="VN034_00035"
     CDS             2399..3325
                     /locus_tag="VN034_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWV59012.1"
                     /translation="MCPLRDALERYITMRRGFGYKLRCEALLLARFVAFMEHQGATII
                     TTKLALDWATKEARPPTWPGRLSVVRTFARHLSSTEPRTQIPPTDMLAARQRRTPHIY
                     TSQEIGSLLDAMLAVRSAKGLHRRTYYCIFGLLTVTGLRIGEALRLKQDDVDLGAGLL
                     IIRDTKFGKSRLVPIHSTTVAVLTDYAKRRDALFATPISPAFFVGEQGRALNHNAIHL
                     IFGNVARRIGLRRPGDAYRGPRVHDLRHSYAVATLLRWYRAGDDVEQRLPILSTYLGH
                     SHTSSTYWYLSACPELMEHAARRLDARWEQAS"
     gene            3322..4323
                     /locus_tag="VN034_00040"
     CDS             3322..4323
                     /locus_tag="VN034_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWV59013.1"
                     /translation="MTRSPSIAPLIERYFAERLMHQRGASPHTVASYRDTFRLLLRYA
                     QGRLRKQPSQLDLADLDAPFIIAFLDDLEAGRAIGAKTRNIRLTAIRAFFRFLAFEEP
                     ASSGQIQRILAIPGKLTEKREVHFLSRPEIDAILAAPDRTGWIGRRDHMLLLFAIQTG
                     LRLSEIASLDRGDIMLGTGAHVRCVGKGRKERRTPLAGQVRAAMQVWLKEPPRGGASA
                     LFPTIHGGRLSPDAIQYLLAKHVRATRAHCPSLRHKRVSPHVLRHTAAMELLQAGVDL
                     SVIALWLGHESILTTQTYLHAHIALKTAALAKLQPHGMKRSARFEPEDRLLAFLDAL"
     gene            complement(4342..5193)
                     /locus_tag="VN034_00045"
     CDS             complement(4342..5193)
                     /locus_tag="VN034_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HWV59014.1"
                     /translation="MDEFAVATSRATQPWKKNGKVRKIVLFGSYARGDWVDEPANGYQ
                     SDYDLLIIVSHDDLTEIADYWYVAEDKILRDAAIGRPVNIIVHTLDEVNRGLNRGEYF
                     WVDIARDGVVLYDLPGSELAAPMPLTAVDAYDMASEYLAKQLPAVDRWLKLAAVSCAE
                     SATDPEWAKNAAFNLHQAAETAYTCYLLVRSQYVPRSHNLKFLRSLAEDRETRLVEAW
                     PRATKVDRRRFELAKRAYVEARYSAAYAIDSDDLQAIRAAVTTLRDTVETVSREWLDG
                     LRQKADL"
     gene            complement(5404..5838)
                     /locus_tag="VN034_00050"
     CDS             complement(5404..5838)
                     /locus_tag="VN034_00050"
                     /inference="COORDINATES: protein motif:HMM:NF026041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HWV59015.1"
                     /translation="MPDQLNLPDWTVVAARPIGTETYEIDAAYDVYPDACLKCGVVGQ
                     LYRHGTKLIRYVDSPVHGRQTFINVKRARYRCRECLGTFMQPLPDMDNERRMTVRCRS
                     YIERQCLLKPNTHVAEDVGINEKVVRQIGKANAARLIAKVDV"
     gene            complement(5839..6486)
                     /locus_tag="VN034_00055"
     CDS             complement(5839..6486)
                     /locus_tag="VN034_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59016.1"
                     /translation="MTAKKTASKFRSRTRILHYVLSFCVTKIFGQEEAMKQHPRTHGG
                     ISGFVPVSDDTPVQVGDLVLLEAAPATKWQIGWLAEMRTMPQGWTDYLIESLEDGSLC
                     WWSDVGICFLKRDSVQANWRWDDRQWAFKDRWFRVCDKARGAFSDRPLFPDFGDGFAV
                     TIGTRTSHRFDDIRPTRTFPDYRKVTKAMMAQCYAECVAERERVVAERKAVGETV"
     gene            complement(6623..7531)
                     /locus_tag="VN034_00060"
     CDS             complement(6623..7531)
                     /locus_tag="VN034_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase and HEPN
                     domain-containing protein"
                     /protein_id="HWV59017.1"
                     /translation="MRTDLDHLPASKQRELERVKAIIFEEFADSIALATMGWKKKGRI
                     DKIILYGSYARGGWVDEPHTAKGYRSDFDMLIIVTDKRLTDKVDVWSKLDDRLARELL
                     IDQTLKTPVNFIVHTLQEVNDGLAHGRYFFMDVARDGIALYESDDKELHQPKPKTPQQ
                     ALDMAREYFEEWMPTAMSFQRNAGYAVKDGDFRLAAFLLHQTTERLYHCVLLVCTFYT
                     PHVHNLGFLRTQAERIDMRLVDAWPRELRTDRSRFEKLKEAYVKARYSKLCRTRHNLD
                     YAERATMPSERGMPPLSRNFAVIGSA"
     gene            complement(7590..7835)
                     /locus_tag="VN034_00065"
     CDS             complement(7590..7835)
                     /locus_tag="VN034_00065"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWV59018.1"
                     /translation="MRMSREDKRRLWRAECAVDRLPPMTRKVFLAIRVEELSYSAIAA
                     RFGISVADVEAHFAVALRVLFQVMDEKDPWWWRFRLW"
BASE COUNT         1496 a         2612 c         2354 g         1570 t
ORIGIN      
        1 aaacgggcgc ggcatgacac gcgggcgtgg caggtgaagc gccgcgaacg cacgcgccag
       61 ctgatcgaac tgggcgggct catcgccaag accgatctcg tcgagctcac cggtgacgat
      121 cgcgccgtga tcctgggctt gctcgtcgaa gccgccgcga cgctacgcgg caaagcgcgc
      181 gatcagcaac tactgctgtg gcgccgccgg ggacggcgcg cctttgcaaa tggggatttg
      241 cagccgcctg cgacaacgat atggcgcacc ggtgaccgat aagaccactg ccatcatctt
      301 gcagaccgtt gaacgcgcgc cgcagtgggt tcgccgcgat ctgtgttcga ccgatcaggc
      361 ggcccggacg caggccgaag agacgctcgc cgccatgatc gccgacgcct tgaacaagac
      421 cgacgagccg cctgcctgac tggctatttc gcttttgctc ggccgcgcgc ggcaggcttg
      481 cgaccaccac cgctaccttt tcggccaagc ccgatctgca acgcaagctc tcggcgcttc
      541 tcggcatagt tgggcgcgac catcggataa tccttcggca agttccactt cgcgcgatag
      601 tcgtcgggcg tcatgccgaa gttggtcatc agatgacggc gcagcatctt gagttttttg
      661 ccgtcttcga ggcagaggat atagtcgggt ttcaccgacg cgcgaaccga cacggctggc
      721 tgctgcggct cgggttccgg ttcggccttc gggccaagac cggcaagcgc ttcatgcacc
      781 gagcggatga ccagcgggat atccgatatc gcgacgctat tgttgctgac atgggcggcg
      841 acaatatcgg cggtcagcgt gaccagcgtt tcggaatctt ccacggcatg tcctttcggg
      901 gaggcggcgc tattatttcg tctgcgcctc tagcagtgcg atgcgctccg aacaaatggc
      961 atgcaccgcg cggcccaatt cttcgacgcg gtcgccgagc cagcgcaatt cctcttcagt
     1021 gatcttgtag tgcttcgatt atgccgagcg gccgactatg ccgagtgggc gcgtgatggc
     1081 cccgctttcc cgtaactttg cggtgatcgg ctcggcatag ttttacgctc ctccctgcac
     1141 ccattgcaga ggaggaagcc atgacgctag atgatttttt gaacggcagc tggacgatcc
     1201 gcgatttgcg cgcgggaccg ttcagggact gcatcgacct gtacgcggcc cggctgcgca
     1261 aagacggcta cagcacggtc acggcaagat tctcgatcag gatcgtcaac ggcttcatcc
     1321 agtggctgac caagcacagc ctcgatcgcg gcgatatcaa cgagcagcta gtcgctcgct
     1381 tttttgagga tgaagatcgg ggctgcggtt tgcgcggcgg cgacccggcg gcgatcgaca
     1441 ggtttatgga ggtcctccgc gaggcgggga ttgttgctcc ggcggcaccg cgttttctcg
     1501 gtcccgcaga tcagatcctt gagcgattcc gcgcacatct cgatcgtcgc cacggtctaa
     1561 atgcacggtc gtgcgcggcc tatgtcaagt tcactcgccc tttcttgcgc gacatgtcga
     1621 tcaccgagcc ggaagacttc gctcggctga caacggccga cgtcctcgga tatgtcgagc
     1681 gacgcgcccg cgatgccagt gcggcgacgg cggttacgat gtgctcacgg ctccgatcgt
     1741 tcctgcgata tctccacgtc gaaggcctga tcgcgcacga tcttgccgcg tgcgtcccct
     1801 cggttaagaa gtggcgcttc acagcgctgc cgacctatct ttcggccgcg cagcttgagc
     1861 gggtgcttca gcactgcgac cagaacacag cgtcagggcg ccgcgactac gcggtcctgc
     1921 tgttgctctc ccggcttgga ctgcgggcac aggaagtcgc gaccctcacg ctcgacgaca
     1981 tcgactggcg cgtcggccag ttccgcattc agggcaaggg acggcaaccg gcgatcatgc
     2041 cgctgcctcc cgacgtcgga gcggccattg ctgcttatct tcagagcggt cgtccggcat
     2101 cggatcaccg ccagctcttc ctgaaggcct acccgccgca ttcggggttc ccgccgacca
     2161 gcggcgtccg ggatattgcc ggcagagcgc tccagcgagc cgggatcggc tgcatcgccc
     2221 atcgcggttc gcatattttc cgccacagtc tcgcgaccga gcttctcggt tccggcgcag
     2281 ccctgaccca gatcagccag gtccttcgcc acaaggatca taatactacc cggatctacg
     2341 cgaaggtcga tctggcgagt ctgcgcaccc tgagcctgcc atggccggga gccgcacaat
     2401 gtgcccgctg cgcgatgcgc tcgagcgcta catcaccatg cggcgcgggt tcgggtacaa
     2461 gctgcgatgc gaggccctgc tgctcgcgcg cttcgtcgcc ttcatggagc accagggtgc
     2521 gacgatcatc acgaccaagc ttgccctcga ctgggccacc aaggaggcga ggccgccaac
     2581 ctggcctgga cgcctgtccg tcgtccgcac cttcgcccgg catttgtcga gcaccgagcc
     2641 acgtacgcag attccgccga ctgacatgct cgcggcaagg cagcggcgaa cgccccatat
     2701 ctatactagc caggagatcg gcagcctgct cgacgcgatg ctggcggttc gctcggcgaa
     2761 gggactgcat cgccggacct attactgcat tttcggcctg cttacggtca ccggattgcg
     2821 gatcggcgaa gcgctccggc tcaagcaaga cgatgtcgat ctcggcgctg gcctcctgat
     2881 catccgcgat accaagttcg gcaagtcccg gctcgttccg atccattcga ccacggtcgc
     2941 cgtgctcacc gattacgcca agcgccgcga cgcactcttc gccacgccga tcagcccggc
     3001 attctttgtc ggcgaacagg gtcgcgcgct aaatcataac gcgatccatc tgatatttgg
     3061 caacgtcgcg cgccggatcg gtctgcgtcg ccctggcgat gcctatcgcg gcccgcgcgt
     3121 tcacgacctg cgccacagct acgcggtcgc cacgctgcta cgatggtatc gggccggcga
     3181 tgatgtcgag cagcgattgc ccatcctgtc gacctatctg gggcacagcc ataccagcag
     3241 cacctattgg tatctgtcgg cctgccccga gctgatggag catgccgcgc ggcggctcga
     3301 tgcccggtgg gagcaggcgt catgacccgc tccccctcca ttgcgccgct gatcgagcgc
     3361 tatttcgctg agcgcctcat gcaccagcgc ggtgccagcc cccacaccgt cgcctcctac
     3421 cgcgacacgt tccgtctgct gcttcgctat gcccagggca ggctccggaa gcagccctca
     3481 cagcttgatc tcgccgatct cgatgccccc ttcatcatcg ccttcctcga cgatctcgaa
     3541 gcgggccgtg cgatcggagc caagacccgc aacatccggc tcaccgcgat acgggccttc
     3601 ttccgttttc tcgcgttcga ggaaccggca agcagcggac agatacagcg cattctcgcg
     3661 atccccggca agctcaccga gaagcgcgag gtccatttcc tgtcgcgccc ggagatcgac
     3721 gcgatcctcg ccgcgcccga tcgaacaggc tggatcggcc gccgcgatca tatgctgctg
     3781 ctctttgcga tccagacggg cctgcgcctt tcggagatcg ccagcctcga tcgcggcgat
     3841 atcatgctcg gcaccggcgc gcacgtccga tgcgtcggta agggccgcaa ggaacggcgc
     3901 acaccgctgg caggccaggt ccgcgctgcg atgcaggtct ggctgaagga gccgcctcgc
     3961 ggcggagcat cagcgctgtt ccccaccatc cacggcggcc gcctcagccc cgacgccatc
     4021 cagtatttgc tcgccaagca cgtccgggcg acacgcgcgc attgcccatc actgcgacac
     4081 aagcgggttt cgccgcatgt ccttcggcac accgctgcga tggaactgct ccaggcgggt
     4141 gtcgacctgt cggtgatcgc gctgtggctc gggcatgagt cgatcctgac gacgcagacc
     4201 tatctccatg cgcatatcgc actgaagacc gccgcgctcg ctaagctcca gccccatggc
     4261 atgaagcggt ccgcgcggtt cgaaccggaa gatcggctgc tggctttcct cgacgcgctg
     4321 tagcctgcgc agccggagcg atcagaggtc ggccttctgt cgaagcccat caagccattc
     4381 gcgcgatacc gtctcgaccg tgtcgcgtag cgtggtcacg gccgcccgga tcgcctgcag
     4441 atcgtcgctg tcgatggcgt atgcagccga gtagcgagcc tcgacataag cgcgcttcgc
     4501 cagttcgaag cgtcggcgat caaccttcgt cgccctcggc catgcctcga cgagccgggt
     4561 ctcccggtcc tcggcgagcg agcgcaaaaa cttcagatta tgcgaacgcg ggacatactg
     4621 gctccgcacc aaaaggtagc aggtataggc agtctctgcg gcctgatgca ggttgaacgc
     4681 cgcattcttg gcccactcag ggtcggtcgc actttcggcg caggacaccg cggctagctt
     4741 gagccaccga tcaactgccg gcagctgctt cgcaaggtat tcactggcca tgtcataggc
     4801 atcgaccgcc gtcagtggca ttggggcagc caactcgctt ccgggaagat cgtagagcac
     4861 gaccccgtcg cgcgcgatgt cgacccaaaa atactcgccc cgattcaacc cgcggttcac
     4921 ctcgtcgagt gtgtggacga tgatgtttac cggtcgcccg atcgcggcat cgcgcaggat
     4981 cttgtcctcc gcgacatacc aatagtccgc gatctcggtg aggtcgtcat ggctgacgat
     5041 gatcagcagg tcatagtccg attgatagcc gttcgcgggc tcgtcgaccc agtcgccgcg
     5101 cgcatagctg ccgaacagaa cgatcttccg gaccttgccg ttcttcttcc acggttgcgt
     5161 cgcgcgcgag gtcgcgacgg caaattcgtc catcaaaatc cgcgtcgcca gcgccagttc
     5221 ttcctgctgc gcaggcggga gatgatcgac atcgtttttc atcgttggag tctcgccatc
     5281 ttcacgcccg gaggcaagac ctcattccgg caaagccgcg ttgatcggcc cggatcggat
     5341 gcttcggtga acggcataca ggtggtctcg cggctgctag catcgagtga tatcgcgcgt
     5401 gcatcacaca tcaaccttag cgatcaggcg cgcagcgttg gccttgccga tctggcgcac
     5461 aaccttctca ttgataccaa catcctcagc aacatgcgta ttcggcttca ggaggcattg
     5521 cctctcgatg taggatcgac agcgcactgt catgcggcgc tcgttgtcca tatcaggcag
     5581 cggctgcatg aaagtgccta ggcactcgcg gcaacgatag cgcgcccgtt taacgttgat
     5641 gaaggtctgt cggccatgga ccggagaatc gacatagcgg atcagcttcg tgccgtgcct
     5701 gtagagttgc ccgacaacgc cgcacttcag gcaagcatcg ggatagacat cataggccgc
     5761 atcgatctcg tatgtttcgg tcccgatcgg ccgagccgcg acaacagtcc aatccggcaa
     5821 attcagctga tctggcattt agacggtttc tccgaccgcc tttcgctcgg caacaaccct
     5881 ctccctttcg gcaacgcatt cagcatagca ctgagccatc atcgctttgg tcaccttccg
     5941 ataatccggg aaagtgcggg ttggccggat atcatcgaaa cgatgggaag tgcgcgtgcc
     6001 gatggtgact gcaaaaccgt ccccaaaatc agggaaaagc ggtcggtcgc tgaaggcgcc
     6061 cctggctttg tcgcaaacac gaaaccagcg gtccttgaac gcccattgtc gatcatccca
     6121 gcgccaattg gcctggacag aatcgcgctt gaggaagcaa attccgacgt cgctccacca
     6181 gcagagggag ccatcttcca aactctcgat gagataatcc gtccagcctt gcggcatcgt
     6241 gcgcatttca gcgagccaac ctatctgcca tttcgtggcc ggcgctgcct ccagcaacac
     6301 caagtcgccc acctgaaccg gcgtatcatc cgacaccggg acgaaaccgc taatgcctcc
     6361 gtgcgtgcgg ggatgctgct tcatggcctc ctcctgcccg aagattttgg tgacgcagaa
     6421 cgaaaggacg tagtgcagga tccgcgttcg cgatctgaat ttcgaagccg tctttttcgc
     6481 cgtcattctg cttcctttcc acacgccaat ccgaagcaag attagcacat gccttcttgc
     6541 tttcaacact aaaatccgaa tggtccgatt tctaatccgg gttgcgatcg tcttgcgcag
     6601 atctattcgc acgaacctga aactatgccg agccgatcac cgcaaagtta cgggaaagtg
     6661 ggggcatccc acgctcgctc ggcatagttg cgcgctcggc ataatccaag ttatgccgag
     6721 ttcggcataa cttcgagtag cgcgctttca cataggcttc cttgagcttc tcgaagcgcg
     6781 agcgatccgt tcttaattcg cgcggccagg catcgacaag tcgcatgtcg atgcgctcgg
     6841 cctgcgtgcg cagaaagcca agattgtgaa cgtgaggcgt gtagaaggtg cagaccaaca
     6901 atacgcagtg gtagaggcgt tcggtagtct gatgcagaag aaaggcagct aagcggaagt
     6961 caccgtcttt gacagcgtat cccgcattac gctggaagct catcgcagtt ggcatccact
     7021 cttcaaaata ctcccgcgcc atatcgaggg cttgctgcgg cgtcttaggc ttaggctggt
     7081 gcagttcctt gtcgtcgctc tcgtagagcg cgatcccgtc gcgcgcgacg tccatgaaga
     7141 aatagcgccc gtgcgccaga ccgtcgttca cttcctgcag agtgtggacg atgaagttga
     7201 ccggcgtctt gagcgtctgg tcgatcaaaa gctcgcgggc caatcggtca tcgagtttcg
     7261 accagacgtc gaccttgtcg gtcagccgct tgtcggtgac gataatcagc atatcgaagt
     7321 cggagcgata gcccttggcg gtatgcggct cgtccaccca gccgccgcgc gcgtagctgc
     7381 cgtaaaggat gatcttgtcg atccggccct tctttttcca gcccatcgtc gcgagcgcga
     7441 tgctgtcggc aaattcctcg aagatgatcg ccttcacgcg ctcgagttcg cgctgcttgc
     7501 tggcaggaag atgatcgaga tcggtacgca tcggttttac cctgtaattg gaagcgccgg
     7561 atggcaagcg ccatccggcg cgcggcctgt caccacagcc ggaaacgcca ccaccatgga
     7621 tccttttcat ccatgacctg aaagagcacg cgcagggcca ccgcgaaatg cgcctcgaca
     7681 tcggcgacgc tgatgccgaa gcgggcagcg atcgctgaat agctcaactc ctcgacgcgg
     7741 atcgcaagaa agaccttgcg ggtcatggga ggcaatcgat caaccgcgca ctcggcgcgc
     7801 cacaagcgac gcttgtcctc gcggctcatg cgcatgacac atattccacg ctcaagacgt
     7861 cggcatcagc gtcgcgatgc tcgatccttc catcacacat gatccttctc cttcctgtca
     7921 ccgcaccccg gcccggaagg gggcggaggt gggcggcagg aaggagccgg cagacccgca
     7981 tcatcggacg ggccgcatcc gcaggaccgc aatggaatgg aggacccgcg ac
//
LOCUS       DATMSU010000002         1833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_85336, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000002 DATMSU010000000
VERSION     DATMSU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85336"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1583
                     /locus_tag="VN034_00070"
     CDS             <1..1583
                     /locus_tag="VN034_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWV59019.1"
                     /translation="AAATMTSTGDPLPAAKAKELGLVDELAGEDSLAADAIAFARAKI
                     ADGPRPTREREVFGDVGVIEQLKAKNAKRWRGFEAPYANLACVEAATRLPFDEGLAFE
                     REQFMKLMFGSQSAAQRHIFFAERQAAKIDGLPKDTALRDVKKVGVIGAGTMGGGISM
                     NFLQKGIPVTIVEMAQDALDRGVGVVRKNYDASAAKGRFKPEQVDQMMGLLTPSLSLD
                     DLADCDLIIEAVYENMDVKKDIFGKLDKIAKPGAILASNTSYLDVDEIATATSRPGDV
                     LGMHFFSPANVMKLLEVVRGEKTAPDALATAMAIGKKIGKVAVVAGVCDGFIGNRMLK
                     PRQMEAMKLLMEGATPAQVDKVHVEFGMPMGPFQMSDLAGVDIGWHRDPNRIESIRDA
                     LAAEGRWGQKKQAGFYDYDEKRNPSESPRVAEIIEEFRKKSGVEKRAITDQEIIERTL
                     YPMVNEGALILSEGKAQRASDIDVVWIYGYGWPVYRGGPMFWAGLEGTDKIVAALEKH
                     GFEIAPLLKEKAEAKSGF"
BASE COUNT          353 a          576 c          628 g          276 t
ORIGIN      
        1 aagccgcggc gacgatgacc tcgaccggcg atccgctgcc cgccgcgaaa gccaaggagc
       61 tcggcctcgt cgacgaactc gcgggcgagg acagccttgc cgccgatgcg atcgcctttg
      121 cgcgcgcgaa gatcgccgac ggcccgcggc cgacgcgtga gcgcgaagtg ttcggcgatg
      181 tcggcgtgat cgagcaattg aaggcgaaga atgcgaaacg ttggcgcggg ttcgaggcgc
      241 cttacgcgaa cctcgcctgc gtcgaggccg cgacgcgcct gcccttcgac gagggtctgg
      301 cgttcgagcg cgagcagttc atgaagctga tgttcggcag ccagtcggcg gcgcagcgcc
      361 acatcttctt cgccgagcgc caggccgcaa agatcgacgg cctgcccaaa gataccgcgc
      421 tccgcgacgt gaagaaggtc ggcgtgatcg gcgccggcac gatgggcggc ggcatctcga
      481 tgaacttcct ccagaagggc atcccggtca cgatcgtcga gatggcgcag gacgcgctcg
      541 accgcggcgt cggcgtggtc cgcaagaatt acgacgcgtc ggcggccaag ggccgcttca
      601 agcccgagca ggtcgaccag atgatgggcc tgctgacgcc gagcctcagc ctcgacgatc
      661 tcgccgactg cgacctgatc atcgaggcgg tctatgaaaa tatggacgtc aagaaggaca
      721 tcttcggcaa gctcgacaag atcgccaagc ccggcgcgat cctcgcgtcg aacaccagct
      781 acctcgacgt cgacgagatc gcgaccgcga cgagccgccc cggcgacgtg ctcggcatgc
      841 acttcttctc gcccgccaat gtgatgaagc tgctcgaggt cgtgcgcggc gagaaaaccg
      901 cgcccgacgc gctcgccacc gcgatggcga ttggcaaaaa gatcggcaag gtcgcggtgg
      961 ttgcgggcgt gtgcgacggc ttcatcggca accgcatgct caagccgcgg cagatggagg
     1021 cgatgaagct gttgatggaa ggcgcgacgc cggcgcaggt cgacaaggtg catgtcgaat
     1081 tcggcatgcc gatggggccg ttccagatga gcgacctcgc cggcgtcgac atcggctggc
     1141 accgcgaccc gaaccgcatt gaaagcatcc gcgacgcgct cgccgccgaa ggccgctggg
     1201 ggcagaagaa gcaggcgggc ttctatgact atgacgagaa gcgcaatccg tcggaaagcc
     1261 cgcgcgttgc ggagatcatc gaagagttcc gcaagaagtc gggcgtcgag aagcgcgcga
     1321 tcaccgatca ggagatcatc gagcgcaccc tctatccgat ggtcaacgaa ggcgcgctga
     1381 tcctgtccga aggcaaggcg cagcgggcat cggacatcga tgtcgtgtgg atctatggct
     1441 atggctggcc ggtctatcgc ggcggcccga tgttctgggc gggactcgaa ggtaccgaca
     1501 agatcgtcgc ggcgctcgaa aaacatggtt tcgaaatcgc gccgctgctc aaggaaaagg
     1561 cggaggcgaa gtcgggcttc tgacgccctc gacttacgaa aaggaggccg tctgtcccgc
     1621 tgcggggcag gcggcctttc ttttgcccac gggcggccgc aggcgtgagc gaaaacttgc
     1681 gattcagggt taaagcgctg gcaacacccg ccgttacggc aaagcatgaa cgccatgtcc
     1741 gcccttgccc tcctgttgct cgccggcagc gcgccggtcg ccgcacccgt ggcgacgccg
     1801 caggtcagcg cggtcggagt ggcgcgggcg cgg
//
LOCUS       DATMSU010000003         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_78, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000003 DATMSU010000000
VERSION     DATMSU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1633
                     /gene="gltX"
                     /locus_tag="VN034_00075"
     CDS             176..1633
                     /gene="gltX"
                     /locus_tag="VN034_00075"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWV59020.1"
                     /translation="MATENQTEVAEATGADVVTRFAPSPTGYLHIGGARTALFNWLFA
                     RHHGGKFLLRIEDTDRARSTDAAIDAILDGMQWLDLDWDGETVYQFARAPRHAEVAHE
                     LLAKGEAYRCYLSQDELAAMRAEAQDKRQPFRVRSPWRDRDDGGDPSVPHVLRLRAPQ
                     EGAVTIADRVQGEVTVQNAELDDFILLRSDGTPTYMLSVVVDDNDMGVTHVIRGDDHL
                     NNAFRQLALLRAMNWREPVYAHVPLIHGADGAKLSKRHGALGVDAYRDEMGYLPEAVN
                     NYLLRLGWGHGDDEIISRAQATEWFDLDHVGRSPSRFDFKKLENLNGHYLREADDARL
                     ADLVAPRVEKLVGRALDGLDRDLLTRAMDALKPRAKTLDEIADGATFLFQPDPLLVDE
                     KAAEVLKAAPEGLLAAVTERLRGLNNWTTEELDAAVRAEAEAAELGLGKLAQPLRAAL
                     TGRTVSPGIFDVLLLLGRDVSLARLDAAQHYPAGA"
     gene            1672..2955
                     /locus_tag="VN034_00080"
     CDS             1672..2955
                     /locus_tag="VN034_00080"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWV59021.1"
                     /translation="MTDTAKITLGDKTVDSPVLSGTVGPDVVDIRKFYAQTGAFTYDP
                     GFTSTASCESQLTYIDGDEGVLLHRGYAIGDLAEHSSFMETCYLLLNGELPNGEELAS
                     FENTITRHTMLHEQLATFYRGFRRDAHPMAVMCGVVGALSAFYPDSTEIHDPRQRMIA
                     SHRLIAKMPTIAAMAYKYSVGQPFVYPDNSLSYTGNFLRMTFGVPAEEYEVNPVVERA
                     LDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLR
                     EIGRPERIPEYIARAKDKDDPFRLMGFGHRVYKNYDPRATVMQKTVREVFDALKVNDP
                     VFEVALQLEEMALNDPYFIEKKLFPNVDFYSGVILSAIGFPTTMFTALFALARTVGWV
                     AQWNEMISDPAQKIGRPRQLYTGPTHRPFVPVDKR"
BASE COUNT          562 a         1071 c          894 g          488 t
ORIGIN      
        1 cacagcccga tccgctcgcg ctcggcctcc agccgcgcct cgaccgcgtc gccaacgcga
       61 ttccgggtgc gaaccgaccc gcctgcaatg ggcgtttgaa gctgccctgt cgccatgcta
      121 ggggcacccc cgatccccgc ccaaccgggt ggcaaagtga gtgaaagagg ccttggtggc
      181 aaccgaaaac caaaccgaag tggctgaagc aacgggcgcc gacgtcgtga ctcgcttcgc
      241 cccctcgccc accggctatc tgcatatcgg cggcgcccgc accgcgctct tcaactggct
      301 gttcgcgcgc caccacggcg gcaaattcct gctgcggatc gaggacaccg accgcgcgcg
      361 ctcgaccgac gccgcgatcg acgcgattct cgatgggatg cagtggctcg atctcgattg
      421 ggacggcgag acggtgtatc agttcgcgcg cgcgccgcgc catgccgagg tcgcgcacga
      481 actgctcgcg aagggcgaag cctatcgctg ctacctcagc caggacgaac tcgccgcgat
      541 gcgcgccgag gcgcaggata agcgccagcc cttccgcgtc cgcagcccgt ggcgcgaccg
      601 cgacgacggc ggcgaccctt cggttccgca cgtcctccgc ctgcgcgcgc cgcaggaagg
      661 cgcggtgacg atcgccgacc gggtccaggg cgaggtcacc gtccagaacg ccgaactcga
      721 cgatttcatc ctgctccgca gcgacggcac gccgacctat atgctcagcg tcgtcgtcga
      781 cgataacgac atgggcgtca cccacgtgat ccgcggcgac gatcatctca acaacgcctt
      841 ccgccaattg gcgctgctgc gcgcgatgaa ctggcgcgag cccgtctatg cgcatgtccc
      901 gctgatccac ggcgccgacg gcgccaagct gtcgaaacgt cacggcgcgc tcggcgtcga
      961 tgcctatcgc gacgaaatgg gctatctccc cgaagcggtg aacaattatc tcctccgcct
     1021 cggctggggg cacggcgatg acgagatcat cagccgcgcg caggcgaccg aatggttcga
     1081 cctcgatcat gtcggccgct cgccgtcgcg cttcgacttc aaaaagctcg aaaatctcaa
     1141 cggccattat ctgcgcgaag ccgatgacgc gcgcctcgcc gatctcgtcg cgccgcgcgt
     1201 cgaaaagctc gtcggccgcg cgctcgacgg tctcgaccgc gacctgctga cgcgcgcgat
     1261 ggacgcgctg aagccgcgcg ccaagacgct cgacgaaatc gccgacggtg cgaccttcct
     1321 gttccaaccg gacccgttgc tggtggacga aaaggcggcc gaagtgctaa aggccgcgcc
     1381 cgaaggcttg ctggccgccg tgaccgagcg actgcgcggg ctgaacaact ggacgacaga
     1441 agagctggac gcggccgtgc gcgccgaagc cgaagccgcc gaactggggc tcggaaaact
     1501 cgcgcaaccc ctacgcgccg cattaaccgg ccgaaccgtt tccccgggaa ttttcgacgt
     1561 gctgctcctg ctcggacgcg atgtcagtct cgcccgactt gacgcagcgc aacattatcc
     1621 ggcaggggcg tagcgactct ctctccccgc cgtcagaaca gggaaaaaaa tatgaccgac
     1681 accgcgaaaa tcacgctggg cgacaagacc gtcgacagcc ctgtcctgtc gggcaccgtc
     1741 ggccccgacg tcgtcgatat ccgtaaattc tacgcccaga ccggcgcctt cacctacgac
     1801 cccggcttca cctcgaccgc cagctgcgaa tcgcaactga cctatatcga cggcgatgaa
     1861 ggcgtgctgc tccaccgcgg ctatgcgatc ggcgacctcg ccgaacattc gagcttcatg
     1921 gaaacctgct acctcctgct caacggcgaa ctgccgaacg gcgaggaact ggcgtcgttc
     1981 gaaaacacca tcacccgcca cacgatgctg cacgaacagc tcgccacctt ctatcgcggg
     2041 ttccgccgcg acgcgcaccc gatggcggtg atgtgcggcg ttgtcggcgc gctcagcgcc
     2101 ttctaccccg attcgaccga aatccacgat ccgcgccagc ggatgatcgc gagccaccgg
     2161 ctgatcgcga aaatgccgac catcgccgcg atggcgtata aatattcggt cggccagccc
     2221 ttcgtctatc ccgacaattc gctaagctac accggcaatt tcctgcgcat gaccttcggc
     2281 gttcccgccg aggaatatga ggtcaatccg gtcgtcgagc gcgcgctcga ccgcatcttc
     2341 atcctccacg ccgaccacga gcagaatgcg tcgacctcga ccgtccgtct cgcgggttcg
     2401 tcgggcgcca accccttcgc ttgcatcgcg gcgggtatcg cctgcctgtg gggcccggcg
     2461 catggcggcg cgaacgaggc cgcgctcaac atgctgcgcg aaatcggccg tccggaacgc
     2521 atccccgaat atatcgcgcg cgccaaggac aaggacgatc cgttccgcct gatgggcttc
     2581 ggccaccgcg tctacaagaa ttacgacccg cgcgcgaccg tgatgcagaa gacggtgcgc
     2641 gaagtgttcg acgcattgaa ggtcaacgat cccgtgttcg aggtcgcgct gcagctcgag
     2701 gaaatggcgc tcaatgaccc ctatttcatc gaaaagaagc tgttcccgaa cgtcgatttc
     2761 tattcgggcg tgatcctctc ggcgatcggt ttcccgacga cgatgttcac cgcgctcttc
     2821 gcgctcgcgc gcaccgtcgg ctgggtcgcg cagtggaacg aaatgatctc ggaccccgcg
     2881 caaaagatcg gccgcccgcg ccagctctac accggcccga cgcaccgtcc gttcgtcccg
     2941 gtcgacaagc gctgagcctt cggtgcatcg aaacaggaaa aggggccgtt ccgcggcccc
     3001 tttttctttg cgcca
//
LOCUS       DATMSU010000004         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_75874, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000004 DATMSU010000000
VERSION     DATMSU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75874"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VN034_00085"
     CDS             <1..300
                     /locus_tag="VN034_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59022.1"
                     /translation="VHALANEPVDALCPRADRLAAALKALVDARADPEGLLDTLTAAD
                     DPALFLRGDIYGTRASTLVAIDGQGSAMIVERRFGPDGVAGGETRLSIDLKRPAE"
     gene            310..1758
                     /locus_tag="VN034_00090"
     CDS             310..1758
                     /locus_tag="VN034_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWV59023.1"
                     /translation="MANRYGCIALLLLAGCGGSGGDGGPVTVPTNAAPIFTSASSVST
                     VENAALSYQAAASDADGDALTYTIAGGADAALFSIGAAGALSFRQAPDFDNPADADAD
                     NVYLVQLSVSDGRTSASLPLSVTVTNSREGIAVRRVFSGFNEPTVLAGIPGDSRFLVG
                     ERDGSIYYFDPATNNRALFRRVGPYDPVSGAPRAMGQYGLMGLAVSPDYATDGTFYAG
                     MDDATKHGSLWAYSHDLSGSGPLTPQNIIYIDWPSGFAAKWLGFGPDNKLYFLTGDHV
                     IIGDGTAMAEDVELGKLFAITKNPNPGGGFSYLVNLAAKELRNPATATFYGSTLLFGD
                     RGNRYFGEVNRFDIGDPMLNYGWDAREGTYGERPAGLDDTEPTLQIAFGTGPKQGRSI
                     TVGPVYLGVIASLQGLLLMADSETGKIWTAPADNLVNGETIEAEGFARREADFVPDAG
                     SIDGIVAMAAMPSGRYYMLDRDGELFEIQPAP"
     gene            complement(1769..2305)
                     /gene="infC"
                     /locus_tag="VN034_00095"
     CDS             complement(1769..2305)
                     /gene="infC"
                     /locus_tag="VN034_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWV59024.1"
                     /translation="MTRRPMAPMPPKNGPRYNEFIASPKVRVIDEEGENLGVMLTAEA
                     IEQAAEVGLDLVEVSPNADPPVCKFLDVGKFKYEAQKKANLARKSQKTQEIKEIKMRP
                     NIDDHDFDVKMKKVFDFLEEGDKVKMTMRFRGREMSHTQLGLNVLQRVAELTSEIAKV
                     EAHPRTEGRQMLMVIAPK"
     gene            complement(2477..>2936)
                     /locus_tag="VN034_00100"
     CDS             complement(2477..>2936)
                     /locus_tag="VN034_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HWV59025.1"
                     /translation="AMDAVLIADSGGNAIRAVPVIKGTGDKQILGTELWNTDAALGGN
                     AAMRGAWFASVSDGLYGQLATKYRTRFGRPPYRLASLGYDSVLLTVRIARDWKPGSNF
                     PASRLLAADGFGGIDGIFRFNNRGIAERALEVSEIGAGGFRVIDPAPTKW"
BASE COUNT          521 a          905 c          906 g          604 t
ORIGIN      
        1 gtccacgcgc tcgccaacga gcccgtcgac gccctatgcc cgcgcgccga tcgtctggcg
       61 gccgcgctga aggcgctggt cgatgcacgc gccgaccccg aagggctgct cgatacactg
      121 accgccgcgg acgaccccgc gctgttcctg cgtggcgaca tctatggcac ccgtgcgtca
      181 acgctggtcg cgatcgacgg gcagggcagc gccatgatcg tcgaacgccg ctttggcccg
      241 gacggcgtcg ccggcggcga aacacgtttg tccattgacc tcaaaagacc agcggaatag
      301 cctgccgaaa tggcaaaccg atatggatgc atcgcgcttc tgcttctagc tggatgcggg
      361 ggcagcgggg gtgatggcgg accggttacg gtaccgacga acgcagcgcc gattttcacc
      421 tccgcatcgt cggtatcgac ggtcgagaat gccgcgctct cctatcaggc ggccgcaagc
      481 gacgccgatg gcgatgcgct gacctatacg atcgctggcg gtgccgacgc ggcgcttttt
      541 tcgatcgggg ccgccggcgc gctctccttc cggcaggcac ccgatttcga caatcctgcc
      601 gatgccgatg cggataatgt ctatctggtc caactatcgg tgagcgacgg acggacaagc
      661 gcttcgttgc cgctttcggt taccgtcacg aacagccgcg aaggcattgc ggtgcgccgc
      721 gtattcagcg gtttcaacga gcccacggta ttggcgggca ttccgggcga ctcgcgtttc
      781 ttggtgggcg agcgcgacgg gtcgatctat tattttgacc cggcaaccaa caatcgcgcc
      841 ttgttccggc gcgtgggacc ctatgatccc gtttcaggcg cgccacgcgc aatgggacaa
      901 tatggtttga tggggctcgc cgtttcgccc gattatgcca ctgacggaac cttttacgcc
      961 gggatggacg acgccaccaa acatggcagt ctttgggcat attcgcatga cttatccggc
     1021 agcgggccgc ttacccccca aaatataata tatatcgact ggccttccgg atttgccgca
     1081 aaatggctgg gtttcggtcc cgacaacaag ctatatttcc tgactggcga ccatgtaatc
     1141 atcggggacg gcactgcgat ggccgaagac gtcgaattgg gtaagctttt tgcgatcacg
     1201 aaaaatccga atcctggcgg gggattttcc tatctcgtga acttggcggc aaaagagctt
     1261 cgaaacccgg caacggccac cttttacggc tcgaccttgt tattcggcga tcgtggcaac
     1321 cgctatttcg gcgaggtcaa ccgtttcgat atcggcgatc ccatgctgaa ctatggctgg
     1381 gatgcgcgtg aaggaacata tggggagcgt ccggccgggc tggacgatac cgagccgact
     1441 ttgcaaattg cgttcggcac gggtcccaaa caaggtcggt cgataaccgt gggtccggtc
     1501 tatctaggcg tgatcgcttc gcttcaaggc ctattgctga tggcagacag cgagaccggc
     1561 aagatttgga cggcccccgc cgacaatctg gttaacgggg aaaccatcga agccgagggc
     1621 ttcgcccgcc gagaagccga tttcgttccc gatgcaggaa gcatcgacgg catcgttgcc
     1681 atggcagcaa tgccgtcggg tcgctattat atgctcgacc gcgacggcga actgttcgaa
     1741 atccagcccg ccccctgatc gcacccgcct atttcggcgc gatcaccatc agcatctgac
     1801 ggccttcggt gcgcggatgc gcctcgacct tcgcgatttc cgacgtcagt tcggcgacgc
     1861 gctggagcac gttcaggccg agctgggtgt ggctcatttc gcggccgcgg aagcgcatgg
     1921 tcatcttgac cttgtcgcct tcctcgagga agtcgaagac cttcttcatc ttcacgtcga
     1981 aatcatgatc gtcgatgttc ggacgcatct tgatctcttt gatttcctgc gtcttctggc
     2041 tcttgcgcgc gaggttcgcc tttttctgcg cctcgtactt gaacttgccg acgtcgagga
     2101 atttgcacac cggcggatcg gcgttgggag atacttccac caggtcgagc ccgacctctg
     2161 ccgcctgctc gatcgcctcg gctgttagca tgacgcccag attttcgcct tcctcgtcga
     2221 tcacgcggac tttgggggag gcgataaatt cattgtaacg cgggccgttc ttgggcggca
     2281 tcggcgccat cgggcgacgg gtcatgggcg gtggtatagc tgtatctcct gaaacatatt
     2341 aaacgcgcgt gcggaatcat cgccggaaca cgcgcgattg tcatatagtg atcgcgcggc
     2401 gggcgtaaag aacgccgcgc ttttccgata gcggttttcg gccaactgtg gccggtctgc
     2461 cactgtcaat cgcggctcac catttggtcg gcgcggggtc gatcacgcga aagccgccgg
     2521 cgccgatttc gctgacctcg agcgcgcgct cggcgatgcc gcgattgttg aagcgaaaga
     2581 tgccgtcgat cccgccgaag ccgtcggcgg cgagcaggcg gctcgccggg aaattgctcc
     2641 ccggcttcca gtcgcgcgcg atgcgcaccg tcagcagcac cgaatcataa ccgagactgg
     2701 cgagccggta cggcggccgg ccgaagcggg tgcgatattt ggtcgcgagc tggccgtaga
     2761 ggccgtcgga gacgctggcg aaccatgccc cgcgcatcgc ggcattgccg ccgagcgccg
     2821 cgtcggtgtt ccagagctcg gtgccgagga tctgcttgtc gcccgtgccc ttgatcaccg
     2881 gcaccgcgcg aatggcgttg ccgccgctgt cggcgatcag caccgcgtcc atcgcg
//
LOCUS       DATMSU010000005         3234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_180108,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000005 DATMSU010000000
VERSION     DATMSU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180108"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VN034_00105"
     CDS             <1..96
                     /locus_tag="VN034_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWV59026.1"
                     /translation="IALGPRILRAETAAIAAVSLWMAQHGDWVGG"
     gene            complement(117..788)
                     /locus_tag="VN034_00110"
     CDS             complement(117..788)
                     /locus_tag="VN034_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV59027.1"
                     /translation="MKLFLGNKAYSSWSLRGWLAVKHSGLPFEEVTVPLYDEDWSNRR
                     EGDEFAPSGGKVPILWDGDDIVVWDSLAIIDYLNEKTGGTKGYWPEDMAARAMARSMA
                     AEMHSSFAALRREHSMNIRRIYPPAELSPEVQADVVRIFQIWAEARARFGGEGDFLFG
                     DWSAADIMFAPVVTRFITYSIPLPRFVAAYAQAVISHPCMQEWIGGAQAEDWVIEAFE
                     GPAEG"
     gene            complement(961..1605)
                     /gene="yihA"
                     /locus_tag="VN034_00115"
     CDS             complement(961..1605)
                     /gene="yihA"
                     /locus_tag="VN034_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWV59028.1"
                     /translation="MTEEIDPILTERARKLFAGPIAFLKSAPALQHLPSPSVPEIAFA
                     GRSNVGKSSLLNALTNRNGLARTSVTPGRTQELNYFDVGSPLVFRLVDMPGYGFAKAP
                     KDVVKKWRFLVNDYLRGRQVLKRTLVLIDSRHGIKDVDRDVLEMLDIAAVSYRLVLTK
                     ADKVKASELEAVQAATEAEARKHPAAHPEVIATSSEKGMGIAELRTAVLEAVES"
     gene            complement(1713..>3234)
                     /gene="yidC"
                     /locus_tag="VN034_00120"
     CDS             complement(1713..>3234)
                     /gene="yidC"
                     /locus_tag="VN034_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWV59029.1"
                     /translation="AVLAEGQRIPIETPAIKGSINLVGARVDDITLSKYRQTIKKDSP
                     AVRLFAPGGTEAAYFASLGWAAQGIAVPNANTVWTASGAKLTPTTPVTLSWANTTGQT
                     FRIEYSIDSNYLITAKQTIANTGTAAVSASSFALIDRLGKPTDPHEVDSWTIHVGPTG
                     YLDGKSVFDTDYDDVEEAPNRTVRYSSAGWLGFTDKYWLAAIVPAKGERVTAAISSPA
                     ANNYQTLFARDFRQIAPGTQVTTTSRIFAGAKEVSILSQYQDDQGITRLSNAIDWGWF
                     EFFEVPIFKLLDWLFKQVGNFGVAIMALTLIIRLLMFPIANRQFSSMAQMRLVQPKMK
                     ALQERHKDDKPKMQQELMKLYKDEKINPLAGCLPIVIQIPIFYALYKVLMLTIEMRHQ
                     PFILWIKDLSAPDPLHILNLFGLLPFTPPSILSIGVLAVILGITMWLQFRLNPQATDP
                     VQQQVFKIMPWLFMFIMAPFAAGLLLYWITNNILSIGQQQWMYRKFPALRAAPAAK"
BASE COUNT          550 a         1049 c         1026 g          609 t
ORIGIN      
        1 atcgcgctcg gcccgcgcat cctgcgcgcc gaaaccgcgg cgatcgccgc cgtcagcctg
       61 tggatggcgc agcacggcga ctgggtgggc ggataggcgg ccgccgttac cagctcctat
      121 ccctcggcgg gtccctcgaa cgcctcgatg acccaatcct cggcctgcgc gccgccgatc
      181 cattcctgca tgcacggatg gctgatcacc gcctgcgcat aggcggcgac gaagcgcggc
      241 agcgggatcg agtaagtgat gaagcgcgtg acgaccggcg cgaacatgat gtcggccgcc
      301 gaccagtcgc caaagaggaa atcgccctcg ccgccgaagc gcgcgcgcgc ctcggcccag
      361 atctgaaaga tacgcacgac atcggcctgc acttcgggcg acagctcggc gggcggatag
      421 atgcgccgga tgttcatgct gtgctcgcgg cgcagcgcgg cgaagctcga gtgcatttcg
      481 gccgccatcg accgcgccat cgcgcgcgcc gccatatcct cgggccaata gcccttcgtc
      541 ccgccggtct tttcgttgag atagtcgatg atcgcgagac tgtcccacac gacgatatcg
      601 tcgccgtccc acaatatcgg cactttgccg cccgacggcg cgaattcgtc gccctcgcgc
      661 cggttcgacc agtcctcgtc gtacagcggg acggtgacct cctcgaacgg cagcccgcta
      721 tgcttgaccg cgagccagcc gcgcaggctc cagctagaat aggccttgtt gccgaggaag
      781 agtttcatgg ggtctcctta tatccctctc cccttggggg agagggttgc gcaggcttgg
      841 cagcttgctg cctagccgaa gctgggtgag gggtgtcaac cgtcgcctgc cctcaccaag
      901 ttccggtagc ggacaagtcc gcaaccttca ctatcctctc ccccaagggg agaggatttt
      961 tcaactctcc accgcctcca gcaccgcggt cctgagctcg gcgatgccca tgcccttttc
     1021 gctgctcgtc gcgatcacct cggggtgcgc ggcggggtgc ttgcgcgctt cggcctcggt
     1081 cgcggcctgc accgcttcga gttcactcgc cttcaccttg tcggccttgg tcagcacgag
     1141 gcggtagctc accgccgcga tgtcgagcat ctcgagcacg tcgcggtcga cgtccttgat
     1201 cccgtggcgg ctgtcgatca gcaccaacgt ccgtttcagc acctgccgcc cgcgcagata
     1261 atcattgacg agaaagcgcc atttcttgac gacatccttc ggcgccttgg cgaaaccgta
     1321 gcccggcatg tcgacgagcc ggaacacgag cggcgagccg acgtcgaaat agttgagttc
     1381 ctgcgtccgc cccggcgtca ccgacgtgcg cgcgagcccg ttccggttcg tcagcgcgtt
     1441 gagcagcgac gatttgccga cgttcgagcg ccccgcgaag gcgatctcgg gcacgctcgg
     1501 gcttggcaga tgctgaagcg cgggcgccga tttcagaaag gcgatcggac ccgcaaacaa
     1561 tttgcgcgcg cgttcggtca gaatgggatc aatttcttcg gtcataaagg ccgtgcccct
     1621 gcgaaggcag gggcccatct cctgttgtct aaatccgaag ccaccggaga tgggcccccg
     1681 ccttcgcggg ggcgcacctc aaatgacgat ggttatttcg ccgccggcgc cgccctcagc
     1741 gccgggaatt tgcggtacat ccactgctgc tgcccgatcg acaggatatt gttggtgatc
     1801 cagtaaagca gcagccccgc cgcgaacggc gccatgatga acatgaacag ccacggcatg
     1861 atcttgaaca cctgctgctg caccgggtcg gtcgcctgcg ggttgaggcg gaactgcagc
     1921 cacatcgtga tacccaggat caccgcgagc acgccgatcg acaggatcga gggcggggtg
     1981 aagggcagca ggccgaacag gttcaatatg tgcagcggat cgggcgccga cagatccttg
     2041 atccacagga tgaacggctg atgccgcatt tcgatcgtca gcatcagcac cttgtacagc
     2101 gcgtagaaga tcgggatctg gatgacgatc ggcagacagc ccgcgagcgg attgatcttc
     2161 tcgtccttat agagcttcat cagctcctgc tgcatcttgg gcttgtcgtc cttgtggcgt
     2221 tcctgcagcg ccttcatctt cggctggacg aggcgcatct gcgccatcga cgagaattgc
     2281 cggttggcaa tcgggaacat caagaggcgg atgatcagcg tcagcgccat gattgcgacg
     2341 ccgaaattgc cgacctgctt gaacagccag tcgagcagct tgaagatcgg cacctcgaaa
     2401 aattcgaacc agccccagtc gatcgcgttc gacaggcgcg tgatgccctg gtcgtcctga
     2461 tattgcgaca ggatgctgac ttccttcgcg cccgcgaaga tgcggctggt ggtcgtcacc
     2521 tgcgtgccgg gcgcgatctg gcggaagtcg cgcgcgaaca gcgtctggta attgttcgcg
     2581 gcgggcgagc tgatcgccgc ggtcacgcgc tcgcccttcg ccgggacgat cgcggcgagc
     2641 caatatttgt cggtgaagcc gagccagccc gccgagctgt aacgcacggt gcggttcggc
     2701 gcttcctcga catcgtcata atcggtgtcg aacaccgact tgccgtcgag atagccggtc
     2761 gggccgacgt ggatcgtcca gctgtcgact tcatgcgggt cggtcggctt gccgagccgg
     2821 tcgatcagcg caaagctgct cgcgctgacg gcggccgtgc cggtgttggc gatcgtctgc
     2881 ttcgcggtga tcagataatt gctgtcgatg ctatattcga tgcggaacgt ctggccggtc
     2941 gtattcgccc agctcagcgt caccggcgtc gtcggggtca gcttcgcgcc gctcgcggtc
     3001 cagacggtgt tggcgttcgg caccgcgatg ccctgcgccg cccagccgag gctcgcgaaa
     3061 taggcggcct cggtgccgcc cggcgcgaac agacgcaccg cgggcgaatc cttcttgatc
     3121 gtctggcgat atttgctgag cgtgatgtcg tcgacgcgcg cgccgacgag gttgatcgac
     3181 cccttgatcg cgggggtttc gatcggaatg cgctggcctt cggcgagcac cgcg
//
LOCUS       DATMSU010000006         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_47479, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000006 DATMSU010000000
VERSION     DATMSU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47479"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2125
                     /locus_tag="VN034_00125"
     CDS             <1..2125
                     /locus_tag="VN034_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HWV59030.1"
                     /translation="GIGIGGTGSVAAKSHDKAATAIRIGSGATTPEIRNAGKIEATTG
                     GKAADSVATAVIVEAGGDVALIRNSGTIAAKTGGDGGIARGIVDLSGTVDTVENSGTI
                     SASGALASSDRNIAIDLSANGNGATVKQTAVASGVTAPSIVGDVRFGGGNDVFDIADG
                     SVKGNSFFGAGDNKLALSGDATYAGNARFGAGSDTMTLAGTSKFTGLADFGGGADTLT
                     IGGTSTFAGTLANSQGLAVSVAGGTFDVGGTATISSLAVTEKGTLAVMLDTGATGTNL
                     QVTGNASFGEESKLALKLSSIEDAEGEHVVLTAGTLTGADKLTASQTLLPFLYKGTLT
                     SNATQLIVDVSRKSTTELGLNRSETGAFDAVLDAVVADAKIEDVFLGITDGDQFRNQL
                     GQMLPEHEGGVFETVTSGSRALSRYLQDPSAPFQDEGKWGYWVNQAVWGTSKSVGDTA
                     SYDVSGWGISLGAEIESDVGNFGGSIGYLSGKDGNGSNANEVSTSQFEGALHWRLRSD
                     GFMANARVSGAPISLKGTRIFRAEAGAEDIEKTMKGKWDATLWSASGSLAYDTRVGGL
                     TLRPMVAVDYFKLKEDGYQETGGGDALDLTVLSRDSDELAVSGTMTLGLEFGGADEYD
                     GWTRFELEGGRRQIVSGMLGATTASFKDGTPFTLVPDDRTSGWVGRFRGIAGNSAFQF
                     AGEVSAEEQQSHIGWAFRASLRVGL"
     gene            2363..2884
                     /locus_tag="VN034_00130"
     CDS             2363..2884
                     /locus_tag="VN034_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV59031.1"
                     /translation="MTHTHDRTDAAQGIEGVLLANRDRIVRFLEVRGAGDGAEDLFQD
                     LWMRLTDRRMGPIADPLPYVMRAANNLMLDRYRSARQRELRDKAWGEASATQSPSSES
                     TLISREQLALVETAIAATGERPARIFRRFRVDGIHQRDIASEMGVSLSTVEADLRKVY
                     AKLAEIRRQFDAG"
     gene            2874..3806
                     /locus_tag="VN034_00135"
     CDS             2874..3806
                     /locus_tag="VN034_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWV59032.1"
                     /translation="MQVERADAEARAIDWLVRQRDPDFTDWDGFANWLAEDGAHAAIY
                     DAVASLDRDLDTLPPSPAPSVVMIPDAQRRPSRRAWFGGAMAAAIVGVVSISGLGLLG
                     DGDRIETLAGEHRTVELADGSKIEINGASVVEIDADRPRFARLEAGEAMFHVVHRDDD
                     PFVVETGGAKIVDLGTAFNVVRRDRQTSVAVSEGIVLYNPDRDNVRLVAGKGIEARDG
                     DRTPPVVRDVDVANVGGWRSGLLVYNGTPLAVVAEDLKRTAGMQVSIAPEATDLSFRG
                     ALIIDKNRDRTIADLAALSGTKAERQGDGWILTR"
     gene            3788..>4105
                     /locus_tag="VN034_00140"
     CDS             3788..>4105
                     /locus_tag="VN034_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STN domain-containing protein"
                     /protein_id="HWV59033.1"
                     /translation="MDFDPLTMNPRLSLAVAAIALCLPTQALAADERAFNVPKGSLSS
                     ALPLISRQAGVSISVADAKLWKARVKPVRGRMSVERAIERLLDGSDARAVRVSATSWR
                     IERR"
BASE COUNT          712 a         1364 c         1399 g          630 t
ORIGIN      
        1 cggtatcggc atcggcggga ccggcagcgt cgcggcgaag tcgcacgaca aggcggccac
       61 agcgatccgc atcggcagcg gcgcgacgac gcccgaaatc cgcaacgcgg gcaagatcga
      121 ggcgacgacc ggcggcaagg ccgcggattc ggtcgcaacg gccgtgatcg tcgaagctgg
      181 cggcgatgtc gcgctgatcc gtaacagtgg caccatcgcc gccaagaccg gcggcgacgg
      241 cggtatcgcg cgcggcatcg tcgatctgtc ggggaccgtc gatacggtcg agaacagcgg
      301 cacgatcagc gcgtccggcg cgctcgcctc ctcggaccgc aatattgcga tcgacctgtc
      361 ggcgaacggc aatggcgcga ccgtgaagca gaccgcggtg gcctcgggcg ttacggcccc
      421 gagcatcgtc ggtgacgtcc gcttcggcgg cggcaacgac gtgttcgaca tcgccgacgg
      481 gtcggtgaag gggaacagct tctttggcgc cggcgacaac aagctcgcgc tgtcgggtga
      541 tgcgacctat gccggcaatg cccgcttcgg cgccggcagc gacacgatga cgctggccgg
      601 aacctcgaag ttcaccgggc tcgccgattt cggcggcggc gccgacacgc tgacgatcgg
      661 cggcacctcg acctttgcgg gcacgcttgc caattcgcag ggcctcgcgg tttcggtagc
      721 cggcggcacc ttcgacgtcg gcggcacggc gacgatctcc tcgctcgcgg tgaccgaaaa
      781 gggcacgctc gcggtgatgc tcgataccgg cgctaccgga accaatcttc aggtcaccgg
      841 caacgccagc ttcggcgagg aatcgaagct cgcgctcaag ctgtcgagca tcgaggacgc
      901 cgagggcgag catgtcgtgc tgaccgccgg cacgctgact ggcgcggaca agctgaccgc
      961 gtcgcagacg ctgctgccct tcctctacaa gggcacgctg acctcgaacg cgacgcagct
     1021 gatcgtcgat gtgtcgcgca aaagcacgac cgagctcggc ctcaaccgtt cggaaactgg
     1081 cgcgttcgac gcggtgctcg acgcggtcgt cgccgatgcg aagatcgagg acgtcttcct
     1141 tggcatcacc gacggcgacc agttccgcaa tcagctcggc cagatgctcc ccgagcatga
     1201 aggcggcgtg ttcgaaaccg tcacctcggg ctcgcgcgcc ctgtcgcgct acctccagga
     1261 tcccagcgca ccgttccagg acgagggcaa gtggggctat tgggtcaacc aggcggtctg
     1321 gggcacgtcg aagagcgtcg gcgatacggc gagctatgac gtcagcggct ggggcatctc
     1381 gctcggcgcc gagatcgaaa gcgacgtcgg caatttcggc ggctcgatcg gctatctcag
     1441 cggcaaggac ggcaacggca gcaacgccaa tgaagtcagc accagccaat tcgaaggcgc
     1501 gctgcactgg cgcctgcgct ccgacggctt catggcgaac gcccgcgtct cgggcgcgcc
     1561 gatcagcctg aagggcacgc gcatcttccg cgccgaagcc ggcgccgagg acatcgaaaa
     1621 gacgatgaag ggcaagtggg acgcgacgct gtggtcggca tcaggctcgc tcgcctatga
     1681 cacgcgcgtc ggcggcctca ccctccgccc aatggtcgcg gtcgactatt tcaagctgaa
     1741 ggaggacggt tatcaggaaa cgggcggcgg tgacgcgctc gacctcacgg tcctgagccg
     1801 cgacagcgac gagcttgccg tgtcgggcac gatgacgctc ggcctcgagt tcggcggcgc
     1861 cgacgaatat gacggctgga cccgcttcga actcgaaggc ggccgccgcc agatcgtcag
     1921 cggcatgctg ggtgcgacga ccgcgtcgtt caaggacggc acgcccttca cgcttgttcc
     1981 cgatgaccgc acgagcggct gggtcggccg tttccgcggc atcgccggca actcggcctt
     2041 ccagtttgcc ggtgaagtct ccgcggaaga acagcaaagc catatcggct gggcgttccg
     2101 tgcgagcttg cgggtcggtc tctagccccc cggggccgac cccgcccgca agggggaagc
     2161 gttaccccta cccatgcttc ccccttgcct agggcatgaa cgaatgaaga agacgatgct
     2221 tgcactgtcc atccccgctt tcccggcacc gcggttgcgc ggtggagcct ggacgctata
     2281 tgacggcgtc atcaggggga cgtatggcgc agggcgaagc ggcgctaccc gacggaagat
     2341 ggcgttccaa agcaagcaaa ccatgaccca tacgcatgat cgaaccgacg ccgcccaggg
     2401 catcgaaggc gtgttgctcg ccaaccgcga ccgtatcgtg cgcttcctcg aggttcgcgg
     2461 tgcgggcgac ggcgccgagg atctgtttca ggatctgtgg atgcggctta ccgaccggcg
     2521 catgggcccg attgcggatc ccctgcccta tgtcatgcgc gccgcgaaca atctgatgct
     2581 cgaccgctac cgctcggcgc gccagcgcga gctgcgcgac aaggcatggg gcgaagcgtc
     2641 ggcgacgcaa agtccgtcga gcgaaagcac gctgatttcg cgcgaacaac tggcactcgt
     2701 cgaaaccgcg attgcggcga cgggcgagcg cccggcccgg attttcaggc gctttcgcgt
     2761 cgacgggatc catcaacgcg atattgccag cgaaatgggg gtcagcctga gcaccgtcga
     2821 agccgatctg cgcaaggtgt atgcgaagct cgccgaaatc cggaggcagt tcgatgcagg
     2881 ttgaacgggc ggacgccgaa gcgcgcgcga tcgactggct ggtccgccag cgcgaccccg
     2941 acttcaccga ctgggacggt tttgccaact ggctcgccga agatggcgcg cacgccgcga
     3001 tttacgacgc cgtcgcgagc ctcgatcgcg acctcgacac gctgccgcct tcacccgccc
     3061 cgtcggtggt aatgatcccc gacgcccagc gccggccttc gcggcgcgcc tggttcggcg
     3121 gcgccatggc cgccgcgatc gtcggggtgg tcagcatctc ggggctcggc ctgttgggtg
     3181 acggcgaccg gatcgagacc ctcgcgggcg aacatcgcac tgtggaactc gccgacgggt
     3241 cgaagatcga gatcaacggc gcctcggtgg tcgaaatcga cgcggatcgt ccgcgcttcg
     3301 cccgactgga agcgggcgag gcgatgttcc atgtcgtgca ccgcgatgac gacccctttg
     3361 tcgtcgagac cggcggcgcg aagatcgtcg atctcggcac ggcgttcaat gtcgtacgcc
     3421 gcgatcgtca gacctccgtt gccgtgtccg agggcatcgt cctctataac cccgaccgcg
     3481 acaatgtccg gctcgtcgcg ggcaagggca tcgaagcgcg cgacggcgac cgcacgccgc
     3541 cggtcgtccg ggatgtcgat gtcgcgaatg tcggcggctg gcgcagcggt ttgctcgtct
     3601 ataacggcac gccgctcgcc gtcgtcgccg aggatctgaa gcgcaccgcc gggatgcagg
     3661 tgagcatcgc gccggaggcg accgatctgt cgttccgcgg cgcgctgatc atcgacaaga
     3721 accgcgaccg cacgatcgcc gacctcgccg cgctgtcggg gacgaaggcc gagcggcaag
     3781 gcgatggatg gattttgacc cgctgaccat gaacccgcgc ctttccctcg ccgtcgcagc
     3841 gatagcgctg tgcctgccga cgcaggcgct ggcggcggac gagcgcgcgt tcaatgtgcc
     3901 caaaggcagc ctgtcctccg ctctcccgct gatcagccgg caggcgggcg tcagtatcag
     3961 cgtcgccgat gcgaagctct ggaaagcgcg cgtcaaaccg gtgcgtgggc gaatgagcgt
     4021 cgagcgggcg atcgagcgcc tgctcgatgg ctccgacgcg cgcgcggtgc gcgtcagcgc
     4081 gacgagctgg cgcatcgaac ggcgc
//
LOCUS       DATMSU010000007        12246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_94878, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000007 DATMSU010000000
VERSION     DATMSU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 12246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12246
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94878"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1002)
                     /locus_tag="VN034_00145"
     CDS             complement(13..1002)
                     /locus_tag="VN034_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWV59034.1"
                     /translation="MTQQSPDTLIDPTRAPTLAGVERAAAKVAALLPQTPLLPLEIDG
                     RTIWCKAECLQPVGAFKIRGAWHRLTDLTPEQANEGVVGVSSGNHAQGVAWAAKRLGI
                     KATIVMPSNAPAMKLAATRKLGAEVVLYDRVTESRDAVAAKLLTERGGTLVHAYGDPW
                     IIEGQGSVGIEAAAQMQARGIDGPDKVIACCGGGGLSAGLALALPNAEIVAVEPEGWD
                     DVTRSLVAGKILSVEDMAYPTECDALQTPETWPINFAVLQARGVKGAVVTRSEVRDAM
                     RLAFEKLHLVVEPGGAAALAAVLAGKVSVTDATLVTLSGGNVDPLKFAEIITE"
     gene            1066..2274
                     /locus_tag="VN034_00150"
     CDS             1066..2274
                     /locus_tag="VN034_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HWV59035.1"
                     /translation="MSKVLVIGAGGVGSVAVHKMAMNPDIFPDITLASRRKFKCDAIA
                     ESVKERTGVTIQTAEVDADHIDATAALIRSIGATHVVNLALPYQDLTIMEACLSTGAH
                     YLDTANYEPRDEAKFEYHWQWAYQDRFKEAGLMALLGSGFDPGVTSVFTTWLRKHHFD
                     RIDTLDILDCNGGDHGQHFATNFNPEINIREVTAVARHWENGDWVETPPMSVKQQFDF
                     EEVGPKNMYLMYHEEIESLKTHLPEIKRIRFWMTFGEAYIQHLTVLQNVGMTRIDPVI
                     YEGREIIPLQFLKAVLPEPSSLGETTKGKTNIGVIATGLGKDGKEKTLYLYNICDHED
                     AFAETGNQAVSYTTGVPAMIGAAMMVTGTWSGDGVFNMEQMDPDPFMDMLNKHGLPWQ
                     VKELDAPLDF"
     gene            2435..3520
                     /locus_tag="VN034_00155"
     CDS             2435..3520
                     /locus_tag="VN034_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59036.1"
                     /translation="MRLAFAIVAAIALSTPAAAQSDDPYAPARAIVADIGKIVTPNGV
                     QETFEVTLGGARQVVNVRGSDRDNPILIFVHGGPGAVEMPFAWAFQRPWEDVFTVVQY
                     DQRGAGRSYPLNDPKALAPTMTPERYRDDAIELIELLKKRYAKRKVVLMGHSWGSIVG
                     LSVAVERPDLLYAYVGVGQGIDFREGEKAGMAWTRAKALAAGNREAVAAIDALAPYPQ
                     GEFTIAKADGWRKYAIPYGSLIYNKPDLKYYFQTPRLSPEYTPADVQAWGKGSAFSVT
                     TLWPRLADVSFNSVRKMDVPIVFLLGRHDYTVPSPVAADWFAKVKAPSKKLVWLEHSA
                     HMPMVEEPGHFFAALLRDVLPLTKETK"
     gene            3517..3870
                     /locus_tag="VN034_00160"
     CDS             3517..3870
                     /locus_tag="VN034_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWV59037.1"
                     /translation="MTAWLAPIGLLFVSNIFMTFAWYGQLGAVSRPIWLAVLIAWGIA
                     FFEYCLVVPANRIGYGVYSAAELKTLQEVITLIIFAGFAVFWLGEKLTLNHLVGFGLI
                     AAGAFFIFKGPIPVS"
     gene            3885..5057
                     /locus_tag="VN034_00165"
     CDS             3885..5057
                     /locus_tag="VN034_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HWV59038.1"
                     /translation="METRAGDPGAFARFDLTRVPSPAFVVDAAKVRANLSVLRHIGDA
                     SGARVLAALKAFSMWSLGPTVAGYLDGVCASGLYEAKLGRGEYGGEVATYCAGYKEAD
                     LPEIAALSDHLIFNSPGQIARFRPLLDELRTKGESFDIGLRINPMHSEGEVAKYDPAA
                     PCSRLGFPISQLLPEHMEGVDGVHMHSLCEQDFPPLERTWDAVQPMLRPFFNQLKWIN
                     FGGGHHVTRADYQVDDLIAFLKQVRASTDCDVMIEPGEAIALDAGILVGEVLDLFDNG
                     MAIGITDISATCHMPDVIEAPYRPAMLDEGSDGMPTRLGGPSCLAGDVIGDYRLPGGA
                     RIGQRFAFLDQAHYSMVKTNTFNGVPLPSIWLWDSESDELKLIREFGYEDFKTRLG"
     gene            complement(5187..6839)
                     /locus_tag="VN034_00170"
     CDS             complement(5187..6839)
                     /locus_tag="VN034_00170"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV59039.1"
                     /translation="MVSRAEVNHTFTDTELDELKAFGTTEPHKAGDLLVEEGAMAPDC
                     IVTLSGHTDIFASTDEGRKRVGWMEHGQFAGDLSVLTGQRHLSRVEMGADGEILRIAH
                     ADFQRLIAGNSHFSDIFVRVLSARREFSKHRGFAVTIIIGAAMDRSVYAMRDLLMKHG
                     VAHRWFDPADGPVAAHLLAERGLTEDQLPVAILGAAEVLVQPTPEQLAAGLGLDLLPD
                     GATADVLVVGSGPGGLAAAVYAASEGLTVIALDSLAPGGQAGTSSKIENYLGFPTGIS
                     GNELARRATVQAQKFGARLVSPVRAASINRDGDAYCLHLADGRKLRSRAVVVASGAKY
                     QSLPIDGIEAYEGRGIYYGATPMEAQLCSDAEVTIVGSGNSAGQGAIYLASVAKKVHV
                     IFRRGSLRETMSEYLVKRLEEHPNIEIIPSTDVVALHGEDRLAGLTYRCRETGDEGHC
                     DCGFLFLFLGASPNTSWLPKEMVCDERGFVKTGTDIAPLELVKAGWSLDRMPSRFETS
                     WPRIYAVGDVRKGSVKRVASSVGEGSVVVSDIHQALAEIGTQ"
     gene            7129..8322
                     /locus_tag="VN034_00175"
     CDS             7129..8322
                     /locus_tag="VN034_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HWV59040.1"
                     /translation="MHQHHSAHGLIQALSPIEPVTLVRPHAARRAARFFIERFPGTTM
                     YAVKANPSPDLLRVLWDSGVTHYDVASIAEVRLVARTLPQATLCFMHPVKAEEAISEA
                     YWKHGVRTFSLDTLDELEKIVRATEGAQDLNLLVRLRVSSDHSKLSLAAKFGAEPDEV
                     AELLMATRQAADALGICFHVGSQAMTPHAYAQAMERVRAAIVAASVTVDIIDVGGGFP
                     SSYPGMEPPPLDAYFETIHRSFESLPISYSAELWCEPGRALSAEYSSLIVRVEKRRGD
                     ELYINDGAYGALFDAAHVGWRFPVSLQRDAGSDAEMVAFSFYGPTCDDLDHMAGPFFL
                     PADIKAGDFIEIGMLGAYGCAMRTKFNGFGADETHVVSDEPMVSLYTGEVEQERRSAT
                     VTKLF"
     gene            8411..9736
                     /locus_tag="VN034_00180"
     CDS             8411..9736
                     /locus_tag="VN034_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59041.1"
                     /translation="MQPFHHLLANNLIANITNFTVWFALTFWTFLETQSVFATGMIAG
                     IYLVLTAMLGIWFGSLVDHHGKRNMMLVSSIASLILYAGALGGFLLDPDIRSAETRSL
                     TLWLFILVSMLAVIVGNIRMIALPTLVTLLVPEDRRDKANGLVGMVSGIGFLTTSAIS
                     GFLVAWGGMAGTLIFAIGFSLVAIAHLLFVHVDEPREADGHAETRRIDLAGTFRVVLA
                     IPGLFALILFSSFNNLLGGVFMALMDAYGLSLMKVEEWGLLWAVTSSAFILSGALIAR
                     TGLGANPVRTLLLVNLAVWAIAAVFTVQSSIILLTIGCFIWMFLGPYAEAAEQTTLQK
                     VVPFERQGRVFGFAQSVELAASPLTAFLIAPLTQFVFVPLMTTGWGADAIGDWYGRGP
                     ERGIAIVFTIAGVLGVVLTLAAFGSRSYRRISAAYAAGNGSSAAAAPAA"
     gene            9811..10971
                     /locus_tag="VN034_00185"
     CDS             9811..10971
                     /locus_tag="VN034_00185"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWV59042.1"
                     /translation="MPMLSGIRIVDLTTVIFGPYATQMLADLGAEVIKVEAPGMGDIS
                     RYLGSGVPDPTMGSIHLTVNRGKRSIALDLKNSEDAAVLRDLIADADVFFHNVRGKAI
                     ARLGFDYEGCRAIKPDIIYVHGTGFGQDGPYADLQAYDDVIQAATGTTSLLPRVDGNP
                     APRYFPSLIADKIAGQFGAQAILAALVHKFRTGEGQAVEVPMFECFAAFMLTEHLRDD
                     TLDPPIGPAGYPRQLDPTRQPFPTRDGYLAIVPYTPESTARLLGLLGSADLLATPEYE
                     AAKAKGQHMPIIYSEIARKTPAKTSAEWLELFAANDIPAMAARDLQDVREDPHLVATG
                     FFRHRTHPDVGAFHEMQPPVKYGAMPPRDLGFAPRVDGDGAAIRGELAIRHD"
     gene            11038..11469
                     /locus_tag="VN034_00190"
     CDS             11038..11469
                     /locus_tag="VN034_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWV59043.1"
                     /translation="MTPNILAPGAVLILWTLIMLVWVAVTRFRALAKSGIDLKAAPPG
                     GRGGDLEGILPPVTNWKSHNYTHLLEQPTLFYAVILILHGVGGYPAYVVWIAWAYVAL
                     RIVHSFWQATVNRIPVRFAIFAASTLCLLALALLAVIATLG"
     gene            11481..11915
                     /locus_tag="VN034_00195"
     CDS             11481..11915
                     /locus_tag="VN034_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWV59044.1"
                     /translation="MDTNAILGPVATLALWSMIMWVWMYATRLPAMQRAKIDTANLVG
                     GTGQNLENVLPPQVQWKAHNYNHLMEQPTVFYAVAIALAVGGMGGGLNTQIAWAYVAL
                     RIVHSLIQVTVNRVMWRFLVFALASLALFALCLHAFMGFVLH"
     gene            complement(11912..>12246)
                     /locus_tag="VN034_00200"
     CDS             complement(11912..>12246)
                     /locus_tag="VN034_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HWV59045.1"
                     /translation="AALNGAANPARALVATEHRDLFRRPLVPAELNRFGAIILDPPRA
                     GAEEQVKQLAASTAPVIAYVSCNPASFARDAKLLIEGGYSLDWVQPVGQFRWSTHVEL
                     AARFSRRA"
BASE COUNT         2115 a         4089 c         3775 g         2267 t
ORIGIN      
        1 gtttccgccg gcctattcgg tgatgatctc ggcgaatttc agcgggtcga cattgccgcc
       61 cgacaaggtg acgagcgtcg catcggtcac cgatactttc cccgccagca ccgccgcgag
      121 cgccgccgcg ccgccgggct cgacgacgag gtgcagcttc tcgaacgcca gccgcatcgc
      181 gtcgcgcacc tcgctgcgcg tcacgacggc gcccttgacg ccgcgcgcct gaagcaccgc
      241 gaagttgatc ggccaggttt cgggcgtctg gagcgcatcg cattcggtcg gataggccat
      301 atcctcgacc gagaggattt tgcccgcgac aagacttcgc gtcacatcgt cccagccttc
      361 gggctcgacg gcgacgatct cggcattggg caacgcgagc gcaagccccg ccgacagacc
      421 gcccccgccg cagcaggcga tcaccttgtc aggcccgtcg atgccgcgcg cttgcatctg
      481 tgccgccgcc tcgatcccga cgctgccctg cccctcgatg atccacggat cgccataagc
      541 atggacgagc gttccaccgc gctcggtgag cagcttcgcg gcgaccgcgt cgcgcgattc
      601 ggtgacgcga tcgtagagca cgacctccgc acccagcttg cgcgtcgcgg cgagcttcat
      661 cgccggcgca ttgctcggca tcacgatcgt tgccttgatc cccagccgct tcgccgccca
      721 cgcgaccccc tgcgcatggt tgccgctcga tacgccgacg acgccctcat tcgcctgttc
      781 gggggtcagg tcggtcagcc ggtgccacgc accgcggatc ttgaaggcac cgaccggctg
      841 caggcactcg gccttgcacc agattgtcct gccgtcgatt tccagcggca gcagcggggt
      901 ttgcggcagc aatgcagcga ctttcgccgc cgcgcgttcg acccctgcga gcgtgggcgc
      961 acgtgtcgga tcgatcaggg tatcggggct ttgctgtgtc atggcttcgt cctatagggc
     1021 gcggcataca acacaagccg cagcatagcg ggcaggagat ttttcatgag caaggttctg
     1081 gtgatcggcg caggcggcgt cggttcggtc gcggtgcaca agatggcgat gaaccccgac
     1141 atctttcccg acatcacgct cgcgagccgc cgcaagttca agtgcgacgc gattgccgag
     1201 tcggtaaagg aacgcacggg cgtgacgatc cagaccgccg aggtcgacgc cgaccatatc
     1261 gacgcgaccg cggcgctgat ccgttcgatc ggtgcgacgc acgtcgtgaa cctcgcgctc
     1321 ccctatcagg acctcacgat catggaggcg tgcctgtcga ccggcgcgca ttatctcgac
     1381 accgcgaatt acgaaccgcg cgatgaggcg aagttcgaat atcactggca atgggcgtat
     1441 caggaccgct tcaaggaggc ggggctgatg gcgctgctcg gttcgggctt cgaccccggg
     1501 gtgacgagcg tcttcacgac ctggctcagg aagcatcact tcgaccgcat cgacacgctc
     1561 gacattctcg actgcaacgg cggtgaccat ggccagcatt tcgcgaccaa cttcaatccc
     1621 gaaatcaaca tccgcgaagt gaccgcggtc gcgcgccact gggaaaatgg cgactgggtc
     1681 gaaaccccgc cgatgtcggt gaagcagcag ttcgacttcg aggaggtcgg gccgaagaat
     1741 atgtacctca tgtatcatga ggaaatcgaa agcctgaaga cccacctgcc cgagatcaag
     1801 cgcatccgtt tctggatgac cttcggcgaa gcctatatcc agcacctcac cgtgctccag
     1861 aatgtcggca tgacgcggat cgatccggtg atctacgagg gccgcgagat catcccgctg
     1921 cagttcctga aagccgtgct ccccgaaccg tcgagcctgg gcgagacgac caaaggcaag
     1981 accaacatcg gcgtcatcgc caccggcctt ggcaaggacg gcaaggaaaa gacgctctac
     2041 ctctacaata tctgcgacca tgaggacgcg ttcgccgaaa cgggcaatca ggcggtgagc
     2101 tataccaccg gcgttcccgc gatgatcggc gccgcgatga tggtcacggg cacgtggagc
     2161 ggcgacggcg tgttcaacat ggagcagatg gatcccgatc ccttcatgga catgctcaac
     2221 aagcatggcc tgccgtggca ggtgaaggaa ttggacgcac cgctcgattt ctgaacacgg
     2281 tccgatccag aaccaccgtc gacgataaaa agccccgttc gtgctgagct tgtcgaagca
     2341 ccgttcttct ttcgatgttg caaagacaga acggcccttc gacaagctca gggcgaacgg
     2401 ggtattttgt gcccgaacat ttgagagaca tcctatgaga ttggccttcg cgatcgtcgc
     2461 ggcgattgca ctttcaaccc ccgccgccgc ccaatccgac gatccctacg cgcccgcccg
     2521 tgcgatcgtc gccgacatcg gcaagatcgt cacgccgaac ggcgtgcagg aaacctttga
     2581 agtcacactc ggtggcgcgc ggcaggtcgt gaacgtccgc ggctcggacc gcgacaatcc
     2641 gatcctgatc ttcgtccacg gcggccccgg tgcggtcgaa atgcccttcg cctgggcctt
     2701 ccagcggccg tgggaggatg tcttcacggt cgtgcaatat gatcagcgcg gtgccgggcg
     2761 cagctatccg ctgaacgacc ccaaggcgct cgcgccgacg atgacgcccg aacgctatcg
     2821 cgacgatgcg atcgaattga tcgagctgct caagaaacgc tatgccaagc gcaaggtcgt
     2881 gctgatgggg cacagctggg gctcgatcgt cggcctgtcc gtcgcggtcg agcgccccga
     2941 ccttctctac gcctatgtcg gcgttggcca gggcatcgat tttcgcgaag gcgaaaaggc
     3001 cggcatggcg tggacgcgcg ccaaggcgct cgcggcgggc aacagggaag ccgtcgccgc
     3061 gatcgacgcg ctcgcgccct atccgcaagg cgagttcacg atcgccaagg ccgacggctg
     3121 gcgcaaatat gcgatcccct acggctcgct catctacaac aagcccgacc tcaaatatta
     3181 tttccagacc ccgcgcctgt cgcccgaata tacgcccgcc gacgtgcagg cgtggggcaa
     3241 gggcagcgca ttttcggtga cgacgctctg gccgcgtctc gccgacgtca gcttcaactc
     3301 ggtgcgcaag atggacgtgc cgatcgtctt tctgctcggg cggcacgact ataccgtgcc
     3361 gtcgccggtg gcggcagact ggttcgcaaa ggtcaaggcg ccctcgaaga agctcgtctg
     3421 gctcgaacat tcggcgcata tgccgatggt cgaggagccg gggcatttct ttgccgcgct
     3481 gctccgcgat gtgctgccgc tgacgaaaga aacgaaatga ccgcctggct cgcgccgatc
     3541 gggctgctgt tcgtctcgaa catcttcatg accttcgcct ggtacggcca gctcggcgcg
     3601 gtgtcgcggc ccatctggct cgcagttttg atcgcttggg ggatcgcctt tttcgaatat
     3661 tgcctcgtcg tgcccgcgaa ccgcatcggc tatggcgttt acagcgccgc cgaactcaag
     3721 accttgcaag aagtcatcac gctgatcata ttcgccgggt tcgcggtctt ctggctcggt
     3781 gagaagctga ccttgaatca tctcgtcggc ttcggactga tcgcggcggg ggcgttcttc
     3841 atcttcaagg gaccgattcc ggtctcatag gaaaatatag cgacatggaa acccgtgccg
     3901 gcgatcccgg agcctttgcc cgcttcgacc tgacccgcgt cccctccccc gccttcgtcg
     3961 tcgatgcagc gaaagtgcgc gccaatctgt cggtgctgcg ccatatcggc gatgcgtcgg
     4021 gtgcccgtgt gctcgccgcg ctcaaggcct tttcgatgtg gtcgctgggc ccgaccgtcg
     4081 ccggctatct cgacggcgtc tgcgcgtcgg gcctttacga agccaagctc ggccgcggtg
     4141 aatacggcgg cgaggtcgcg acctattgcg ccggctacaa ggaagccgat ctgcccgaga
     4201 tcgcggcgct gtccgaccat ctgatcttca attcgcccgg ccagatcgcg cgcttccgtc
     4261 ccctcctgga cgagcttcgc accaagggcg agagcttcga catcggactg cgcatcaacc
     4321 cgatgcacag cgagggcgag gtcgcgaaat atgatcccgc cgcgccgtgc agccggctcg
     4381 gcttcccgat cagccaattg ctccccgaac atatggaagg agtcgacggc gtccatatgc
     4441 acagtctctg cgaacaggat ttccccccgc tcgagcgtac ctgggacgcg gtgcagccga
     4501 tgctgcgccc cttcttcaac cagctcaaat ggatcaactt cggcggcggc caccatgtca
     4561 cgcgcgccga ttatcaggtt gacgacctga tcgccttcct gaagcaggtt cgcgcatcga
     4621 ccgactgcga cgtgatgatc gaacccggcg aggcgatcgc gctcgacgcc ggcatcctcg
     4681 tcggcgaagt cctcgacctg ttcgacaatg gcatggcgat cggcatcacc gacatttcgg
     4741 cgacctgtca catgcccgac gtgatcgaag cgccctatcg cccggcgatg ctggatgaag
     4801 gcagcgacgg catgcccacg cgcctcggcg gcccgtcgtg cctcgcaggc gacgtgatcg
     4861 gcgactatcg cctccccggc ggggcgcgca tcggccagcg cttcgccttc ctcgaccagg
     4921 cccattattc gatggtcaaa accaacacat tcaacggcgt accgctgccg tcgatctggc
     4981 tgtgggacag cgagagcgac gaactaaagc tgatccgcga attcggctac gaggatttca
     5041 agacgcggct ggggtaattg atcctccctg tcgcgcagcg atgaggaggt ggcagcgcga
     5101 agcgctgacg gaggggccat aacgccgccc ctccaccacc cgctacgcgg tcggtccccc
     5161 tccccatgcc ttcggcacag ggaggattat tgggtgccga tctccgccag cgcctgatgg
     5221 atgtcgctga ccacgaccga cccctcgccg accgatgacg cgacgcgctt caccgatccc
     5281 ttgcggacgt cgccgacggc gtagatgcgc ggccagctcg tctcgaaccg gctcggcatg
     5341 cggtcgagcg accagcccgc cttgacgagt tcgagcggcg cgatgtcggt gcctgtcttc
     5401 acgaagccgc gctcgtcgca caccatttcc ttgggcagcc agctcgtatt cgggctcgcg
     5461 ccgaggaaga ggaacaggaa gccgcaatcg caatgcccct cgtcgccggt ttcgcggcag
     5521 cgataggtca gcccggcaag ccggtcctcg ccgtgcagcg cgacgacgtc ggtcgacggg
     5581 atgatttcga tgttcggatg ctcttcgagc cgcttgacga gatattcgga catcgtctcg
     5641 cgcaggctgc cgcgacggaa aatgacatgg acctttttcg cgacgctcgc gagatagatc
     5701 gcgccctgcc ccgccgaatt gcccgacccg acgatcgtca cctcggcgtc gctgcacagc
     5761 tgcgcctcca tcggggtcgc gccataatag atgccgcggc cttcataggc ttcgatcccg
     5821 tcgatcggca ggctctggta cttcgcgccg ctcgcgacaa ccaccgcgcg gctcctgagc
     5881 ttgcggccgt cggcgaggtg caggcaatag gcatcgccat cgcggttaat cgaggcggcg
     5941 cgcaccggcg acaccagccg cgcaccgaat ttctgcgcct gcaccgtcgc gcggcgcgcc
     6001 agctcgttcc ctgaaatccc ggtcggaaag ccgagataat tttcgatctt cgacgaggtg
     6061 ccggcttggc cgcccggcgc gagcgaatcg agcgcgatta ctgtcagccc ttcggacgcg
     6121 gcatagaccg ccgcggccag cccgccgggc ccgctgccca cgacgaggac atcggcggtc
     6181 gcgccatcgg gaagcagatc gaggccgagt cctgccgcaa gctgttcggg cgtcggctga
     6241 accagcactt cggccgctcc gaggatcgct accggcagct gatcctcggt cagcccgcgc
     6301 tcggcaagca gatgcgcggc taccgggccg tcggcggggt cgaaccagcg atgcgcgacg
     6361 ccatgcttca tcaacaggtc gcgcatcgca tagacgctgc ggtccatcgc cgcgccgatg
     6421 ataatcgtga ccgcaaaacc ccgatgcttg ctgaattcgc gccgcgccga cagcacgcgg
     6481 acgaagatat ccgaaaaatg gctgttgccc gcgatcagcc gctggaaatc ggcgtgagcg
     6541 atgcggagta tctccccgtc ggcgcccatt tcgacgcgcg acaggtggcg ctgcccggtc
     6601 agcaccgaca ggtcgcccgc gaattggccg tgttccatcc agccgacgcg ctttcgcccc
     6661 tcgtcggtcg acgcgaagat gtcggtgtgg cccgacaggg tgacgatgca gtcgggcgcc
     6721 atcgcgccct cctcgaccag caggtcgccg gccttgtgcg gctcggtcgt gccgaatgcc
     6781 ttcagttcgt cgagttcggt atcggtgaag gtgtgattca cttcggcgcg ggagaccatc
     6841 aaagcttcgc cctgtgttcg tcatcccgat cgctgccggg gtgactcgat gaaatgggat
     6901 gagcccgtcc ctagcgcggc accagcgtct tgtctcgcat ttgcgcggct tggcgcggac
     6961 cggccccggt tcgcccccaa ctgttgccct ggcgttacaa aattgcgcca aagggtcttt
     7021 acagggggga cccccctcca tatatgcgct ctccgctgcc ccagggggac ttccaataac
     7081 cgcaaggcag ccttggatgt ttaacgttat ttttgggggt cccttgagtt gcaccagcat
     7141 catagcgccc acgggctgat tcaggcactt tcgccgatcg aacctgttac gcttgtccgc
     7201 ccgcacgccg cgcggcgcgc agcgcgtttc ttcatcgagc gatttcccgg cacgaccatg
     7261 tatgcggtca aggcgaatcc gtcgcccgac ctgctgcgtg tgctgtggga ttcgggtgtc
     7321 acccattatg acgtcgcctc gatcgccgag gtgcgcctgg ttgcccgcac gctgccgcaa
     7381 gcgacgctct gcttcatgca cccggtaaag gccgaggaag cgatttccga agcctattgg
     7441 aagcatggcg tgcgcacctt ctcgctcgac acgctcgacg agctcgaaaa gatcgtccgt
     7501 gcgaccgaag gcgcgcagga tctgaacctg ctcgtgcgcc tccgcgtctc gtcggaccat
     7561 tcgaagctca gcctcgccgc caagttcggc gccgagcccg acgaggtcgc cgaactgctg
     7621 atggcgacgc gtcaggcggc cgacgcgctc ggcatctgct ttcacgttgg cagccaggcg
     7681 atgacgccgc acgcttatgc gcaggcgatg gaacgcgtcc gcgccgcgat cgtcgcggcg
     7741 tcggtcaccg tcgacatcat cgatgtcggg ggcggtttcc cgtcgtctta cccgggcatg
     7801 gaaccgccgc cgctcgacgc ttatttcgag acgatccatc gcagcttcga aagcctgccg
     7861 atcagctatt cggccgaact ctggtgcgag ccgggccgcg cgctgtcggc cgagtatagc
     7921 tcgttgatcg tgcgtgtcga aaagcgccgc ggcgacgagc tctatatcaa cgacggcgcc
     7981 tatggcgcgc tgttcgacgc cgcgcatgtc ggctggcgct tccccgtctc gctgcagcgc
     8041 gacgcgggca gcgacgccga gatggtggcg ttcagcttct acggcccgac gtgcgacgat
     8101 ctcgaccata tggcgggccc cttcttcctg cccgccgaca tcaaggccgg cgatttcatc
     8161 gagatcggga tgctcggcgc ctacggctgc gcgatgcgga ccaagttcaa cggcttcggc
     8221 gccgacgaga cccatgtcgt cagcgacgaa ccgatggtca gcctctacac tggcgaagtc
     8281 gaacaggaac gccgtagcgc gacggtgacc aaattgttct gacgcaaagc aaacggaaaa
     8341 aggaaagggc cggcgcagcg atgcgaccgg ccctttcgct tttgggcggg ggcgctagat
     8401 tgcgccgccc atgcagccct ttcatcatct cctcgccaat aatctgatcg ccaacatcac
     8461 caatttcacg gtgtggttcg cgctgacctt ctggaccttt ctcgaaaccc agtcggtgtt
     8521 cgcgacgggg atgatcgcgg gcatctatct cgtgctgacg gcgatgctcg gtatctggtt
     8581 tggcagcctt gtcgaccatc atggcaagcg caacatgatg ctggtttcga gcatcgcatc
     8641 gctgatcctc tacgcgggcg cgctcggcgg cttcctgctc gatcccgaca tccgcagcgc
     8701 cgaaacgcgg agcctgacgc tctggctctt catcctcgtt tcgatgctgg cggtgatcgt
     8761 cggcaatatc cggatgatcg cactgccgac gctggtcacc ctgctcgttc ccgaagaccg
     8821 gcgcgacaag gcgaacggac ttgtcggcat ggtcagcggg atcggcttcc tcaccacgtc
     8881 ggcgatcagc ggctttctcg tcgcgtgggg cggcatggcc ggaacattga tctttgcgat
     8941 cggcttttcg ctggtggcga tagcgcacct gctcttcgtc catgtcgacg aaccgcgcga
     9001 ggccgacggc cacgccgaaa cgcgccgcat cgatcttgcg ggaaccttcc gcgtcgtgct
     9061 cgccattccg ggtctctttg cgctgatcct cttctcatcc ttcaacaatc ttctcggcgg
     9121 ggtcttcatg gcgctgatgg atgcctatgg cttgtcgttg atgaaggtcg aggaatgggg
     9181 cctcctctgg gccgtgacgt cgagcgcctt catcctcagc ggcgcgctga ttgcgcgcac
     9241 cgggctcggc gcgaatccgg tccgcaccct gctcctcgtc aacctcgccg tctgggcgat
     9301 cgccgcggtc ttcaccgttc agtcgtcgat catactgctc acgatcggct gcttcatctg
     9361 gatgttcctc ggcccctatg ccgaagcggc cgaacaaacg acactgcaaa aggtcgtgcc
     9421 gttcgagcgg cagggccggg tgttcggttt cgcccagtcg gtcgaactcg cggcctcgcc
     9481 gctgaccgcc ttcctgatcg caccgctcac gcagttcgtc ttcgtcccgc tgatgacgac
     9541 cggatggggc gccgacgcaa tcggcgactg gtacggccgc ggccccgagc gcggcatcgc
     9601 gatcgtcttc acgatcgccg gcgtccttgg cgtggtgttg acgctcgcgg cgttcggctc
     9661 gcgatcctat cgccggattt cggcggccta tgctgccgga aatggcagta gcgccgcagc
     9721 cgctcctgcc gcttagggaa agttgcggaa gccccttccg ttttactttg caatcattct
     9781 atcctgctcg cgtcaggaag aggagaagcc atgcccatgc tttcggggat ccgcatcgtc
     9841 gaccttacca cggtcatctt cggaccctat gcgacgcaga tgctcgccga tctcggcgct
     9901 gaggtgatca aggtggaggc gccgggcatg ggcgacatct cgcgctacct cggcagcggc
     9961 gttcccgacc cgacgatggg atcgatccac ctcaccgtga accgcggaaa acgctcgatc
    10021 gcactcgatc tcaaaaattc cgaagacgcc gcggtgctcc gcgacctgat cgcggacgcc
    10081 gacgtcttct tccacaatgt gcggggcaag gcgatcgcgc ggctcggctt cgattatgaa
    10141 ggctgccggg cgatcaagcc cgatatcatc tatgtccatg gcaccggctt cgggcaggac
    10201 ggcccctacg ccgatcttca ggcctatgat gacgtcattc aggcggcgac gggcaccacc
    10261 agcctgctcc cgcgcgtcga cggcaacccc gctccgcgct atttcccgtc gctgatcgcc
    10321 gacaagatcg cgggccagtt cggcgcacag gcgatcctcg ccgcgctcgt ccacaagttc
    10381 cgaaccggcg aggggcaagc ggtcgaagtg ccgatgttcg aatgcttcgc cgccttcatg
    10441 ctcaccgaac atctgcgcga cgatacgctc gacccgccca tcggccccgc cggctatccg
    10501 cgccagctcg acccgacgcg ccagcctttc ccgacgcgcg acggctatct cgcgatcgtc
    10561 ccttacaccc ccgaatcgac ggcgcggctg ctgggcctgc tggggagtgc cgacctgctc
    10621 gctacgcccg aatatgaggc ggcaaaggcc aaagggcagc acatgccgat catctacagc
    10681 gagatcgcgc gcaagacgcc ggcaaagacc agcgccgaat ggctggaact gttcgccgcc
    10741 aacgacatcc ccgcgatggc ggcgcgcgac cttcaggacg tcagggaaga cccgcatctg
    10801 gtcgcgaccg gatttttccg ccatcgcacg catcccgatg tcggcgcctt tcacgaaatg
    10861 cagccgccgg tgaaatatgg cgcgatgccg ccccgcgact taggcttcgc gccgcgcgtc
    10921 gatggcgacg gcgcggcgat ccgcggcgaa ctcgcaatcc ggcacgactg attttggcaa
    10981 ttgcatatcg gcgcgccgtc cctagcatcg ccgccgtacg acaaaagggg gagccatatg
    11041 acgccgaaca tcctcgcacc gggcgcggtg ctgatcctgt ggacgctcat catgctggtt
    11101 tgggtcgccg tgacgcgttt ccgcgcgctc gccaagtcgg gtatcgacct gaaggcagcg
    11161 ccgccgggtg gccgcggcgg cgaccttgaa ggcatattgc cgcccgtcac caactggaaa
    11221 tcgcacaatt acacgcatct gctcgaacag ccgacgctct tctatgccgt catcctgatc
    11281 ctgcacggcg tcggcggcta tcccgcttat gtcgtgtgga tcgcctgggc ctatgtcgcg
    11341 ctacgtatcg tgcacagctt ctggcaggcg acggtgaacc gcataccggt gcgctttgcg
    11401 atcttcgcgg cgtcgacctt gtgcctgctg gctctcgcac tgctcgcggt aatcgccact
    11461 ttgggttgag ggggagagtt atggatacga acgctatttt ggggccggtc gcgacgctcg
    11521 cgctgtggtc gatgatcatg tgggtgtgga tgtacgcgac gcgcctgccc gcgatgcagc
    11581 gcgcaaagat cgataccgca aacctcgtcg ggggcaccgg acagaatctg gaaaatgtcc
    11641 tgccgccgca agtgcagtgg aaggcgcata attataacca tctgatggaa cagccgacgg
    11701 tcttctatgc cgtcgcgatc gcgctggcgg tcggcggcat gggcggcggg ctcaacaccc
    11761 agatcgcctg ggcctatgtc gccctgcgca tcgtccacag cctgatccag gtgacggtca
    11821 atcgcgtcat gtggcgcttc ctcgtcttcg cgctggcaag cctcgcgcta ttcgcgctct
    11881 gcctgcatgc cttcatgggt tttgtgctgc attaagcacg ccgcgaaaag cgcgccgcca
    11941 gttccacatg ggtcgaccag cggaactggc cgacaggctg gacccagtcg agcgaatagc
    12001 cgccctcgat cagcagtttc gcatcgcgcg cgaagctcgc gggattgcag ctgacatagg
    12061 cgatcacggg cgcggtcgac gccgcgagct gcttcacctg ctcctcggcc ccggcacgcg
    12121 gcgggtcgag gatgatcgcg ccgaaacggt tcagctcggc gggaacgagc gggcggcgga
    12181 acaggtcgcg atgctcggtc gcgaccagcg cgcgggcggg gttcgcggcg ccgttcagcg
    12241 cggcgg
//
LOCUS       DATMSU010000008         4091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_198, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000008 DATMSU010000000
VERSION     DATMSU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1768
                     /gene="ctaD"
                     /locus_tag="VN034_00205"
     CDS             95..1768
                     /gene="ctaD"
                     /locus_tag="VN034_00205"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWV59046.1"
                     /translation="MTDIAATAPAHGHADHAHDDHDHDTPSFFVRWFMSTNHKDIGTL
                     YLIFAIIAGIVGGALSGMMRLELAHPGIQYLGSWAQALGGAQDLTAAKHFWNVMITAH
                     GLIMVFFMVMPAMIGGFGNWFVPLMIGAPDMAFPRMNNVSFWLTAVAFVMLVGSMFVP
                     GGSGNGAGTGWTVYAPLSTSGSVGPAVDMAIFSLHLAGAASILGAINFITTIFNMRAP
                     GMTLHKMPLFVWSVLVTAFLLLLALPVLAAAITMLLTDRNFGTTFYDAAGGGDPVLYQ
                     HLFWFFGHPEVYIMILPGFGMVSQIISTFSKKPVFGYLGMAYAMVAIGVVGFIVWAHH
                     MFTVGMSVNLKMYFTAATMVIAVPTGIKIFSWIATMWGGTLSFKTPMMWSLGFIFMFT
                     VGGVTGVVLANGGVDTNLHDTYYVVAHFHYVLSLGAVFSLFAGFYYWFPKMSGRMYNE
                     ALGQLHFWVFFIGVNVMFFPQHFLGQQGMPRRYPDYAEAYAYWHHISSLGYVIMGVGM
                     LFFFVNILYSLFAGKKAADNPWGEGATTLEWTLSSPPPFHQFNELPRIA"
     gene            1829..2740
                     /locus_tag="VN034_00210"
     CDS             1829..2740
                     /locus_tag="VN034_00210"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWV59047.1"
                     /translation="MAQSLTHGETALPADWRDLFALTKPRVMSLVVFTALCGLLAAPG
                     HVPPVLAFSSILAIALGAGASGALNQWYEAGIDAKMKRTAGRPLPAGRLDPQTALQFG
                     VGLAAFSLFLMLFASNWQATLLLAASILFYVFVYTMWLKPRTPQNIVIGGAAGAFPPL
                     IGWVAATGSIAPLPVLLFLLIFLWTPPHFWALALFMRSDYAAAGIPMMPVVAGEKSTR
                     RQILLYALIMAAAAIAPWPLGYTGALYGWTAVVLSAVFVLLSVQVGTRTTGEGDLMQP
                     EKRLFAYSIAYLFILFGAVVADHWWPL"
     gene            2737..2886
                     /locus_tag="VN034_00215"
     CDS             2737..2886
                     /locus_tag="VN034_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59048.1"
                     /translation="MTEETPNEPFDEVEYRRRQRSRANLMAWMLGALAILFFFITIAK
                     MQIFS"
     gene            2883..3458
                     /locus_tag="VN034_00220"
     CDS             2883..3458
                     /locus_tag="VN034_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWV59049.1"
                     /translation="MTFFPVIGRMSPNAKTASLAALMALAMTGLGFAAVPLYDLFCRV
                     TGFGGTTQRYDPVAAAAEPEILSQTVSVRFDANVSPNLPWKFYPEHPTDTVSIGARDM
                     AIFIAENNSAHPVVGTASFNVTPDQAGKYFTKIQCFCFTQQRLEPGQQMRMPVLFFVD
                     PKIMDDPDARDVQEITLSYTFHPVDEGKKAS"
     gene            3539..>4091
                     /locus_tag="VN034_00225"
     CDS             3539..>4091
                     /locus_tag="VN034_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWV59050.1"
                     /translation="MSGAKHHDYHLVNPSVWPLIGSVAALVMFFGLVMAMHADYFGGI
                     GKWVLGLGFMGVIATFFSWWSDVINEAHAGDHTPVVQLHLRYGMILFIASEVMFFVGW
                     FWAWFDFSLFPVPIEYAEGAVTSLFGQDGAAAITMWPPKGIEVIDPFSLPLLNTLILL
                     CSGTTITWAHHALIHGDREGLKKG"
BASE COUNT          651 a         1353 c         1232 g          855 t
ORIGIN      
        1 ggccgccgcc gccccgaccg aagcacccga agctgcaccg gcagcggcgc ccgccgccgc
       61 tccggccgcc aagaaataac aaggggtccg acagatgacc gatattgctg caactgcacc
      121 cgcccatggc catgccgatc acgcgcatga cgaccatgac cacgatacgc cgagcttctt
      181 tgttcgctgg ttcatgtcga cgaaccacaa ggacatcggc accctctacc tgatcttcgc
      241 gatcatcgcc gggatcgtcg gcggcgcgct ttcgggcatg atgcgcctcg agctcgcgca
      301 tcccggcatc cagtatctgg gcagctgggc gcaggccctc ggcggcgcgc aggatctgac
      361 ggctgccaag catttctgga acgtgatgat caccgcgcac ggcctgatca tggtgttctt
      421 catggtcatg cccgcgatga tcggcgggtt cggcaactgg ttcgtcccgc tgatgatcgg
      481 cgcgcccgac atggcgttcc cgcgcatgaa caacgtgtcc ttctggctga cggcggtcgc
      541 gttcgtcatg ctcgtcggat cgatgttcgt tccgggcggc agcggcaatg gcgcaggcac
      601 cggctggaca gtctatgcgc cgctgtcgac cagcggctcg gtcggccctg cggtcgacat
      661 ggcgatcttc tcgctccacc tcgcgggtgc ggcgtcgatc ctcggggcga tcaacttcat
      721 caccaccatc ttcaacatgc gcgcgccggg catgacgctg cacaagatgc cgctgttcgt
      781 gtggtcggtg ctcgtcaccg ccttcctgct gctgctcgcg ctgccggtgc tcgccgcggc
      841 gatcaccatg ctgctgaccg accgcaactt cggcaccacc ttctatgatg cggccggcgg
      901 cggcgatccc gtcctctacc agcatctttt ctggttcttc ggccaccccg aagtgtacat
      961 catgatcctg ccgggcttcg gcatggtcag ccagatcatc tcgaccttca gcaagaagcc
     1021 ggtgttcggc tatctcggca tggcatacgc catggtcgcg atcggcgttg tcggcttcat
     1081 cgtgtgggcg caccacatgt tcacggtcgg catgagcgtg aacctcaaga tgtacttcac
     1141 cgccgcgacg atggtcatcg cggttccgac gggcatcaag atcttcagct ggatcgccac
     1201 catgtggggc ggcacgctga gcttcaagac gccgatgatg tggtcgctcg gcttcatctt
     1261 catgttcacc gtcggcggtg tgaccggcgt cgtgctcgcc aatggcggcg tcgacaccaa
     1321 tttgcacgac acctattatg tcgtcgcgca cttccactat gtgctgtcgc tgggcgcggt
     1381 cttctcgctc ttcgccggct tctattactg gttcccgaag atgtcgggcc ggatgtacaa
     1441 cgaggcgctc ggccagctgc acttctgggt cttcttcatc ggcgtgaacg tcatgttctt
     1501 cccgcagcac ttcctgggcc agcagggcat gccgcgccgt taccccgact atgcggaagc
     1561 ctatgcctat tggcatcaca tctcgtcgct cggctatgtg atcatgggcg tcggcatgct
     1621 cttcttcttc gtgaacatcc tctactcgct gttcgcgggc aagaaggccg ccgacaatcc
     1681 gtggggcgaa ggcgcgacga cgctcgaatg gacgctgtcg agcccgccgc cgttccacca
     1741 gttcaacgaa ctgccgcgta tcgcctgatg tgtgttgccc cctgccgttt gatggcaggg
     1801 ggcgcacgac tttcgggccg gagtaaatat ggcgcagagc ctgacccacg gcgaaacggc
     1861 actacccgcc gattggcgcg acctgttcgc gctgacgaag ccgcgcgtga tgtcgctggt
     1921 ggtgtttacc gccttgtgcg gcctgctcgc ggcgccgggg catgtgccgc cggtgctcgc
     1981 tttctcgtcg atcctcgcga tcgcgctggg ggcaggggct tcgggcgcgc tcaaccaatg
     2041 gtatgaagcc ggaatcgacg cgaagatgaa gcggaccgcg ggccggccgc ttcccgccgg
     2101 gcgcctcgac ccgcagaccg cattgcagtt cggcgtcggg cttgccgcct tttcgctgtt
     2161 cctgatgctg ttcgcgtcga actggcaggc aacgctgttg ctcgccgcct cgatcctttt
     2221 ctacgtcttc gtctatacga tgtggctgaa gccgcggacc ccgcagaata tcgtcatcgg
     2281 cggcgcggcg ggcgcctttc cgccgctgat cggctgggtc gccgcgacgg ggtcgatcgc
     2341 gccgctgccg gtgctgctgt tcctgctcat cttcctgtgg acgccgccgc atttctgggc
     2401 gctcgcgctg ttcatgcgct cggactatgc cgccgcgggc atcccgatga tgcccgtcgt
     2461 cgcgggtgag aaatcgacgc ggcgccagat cctcctctac gcattgatca tggccgccgc
     2521 cgcgatcgcg ccgtggccgc tcggctacac cggcgcgctt tacggctgga ccgcggtggt
     2581 gctctcggcc gtgttcgtcc tgctgtcggt tcaggtcggt acgcgcacca cgggcgaggg
     2641 cgacctgatg caacccgaaa agcgcttgtt cgcttattcg atcgcttatc ttttcattct
     2701 gttcggcgct gtcgtcgccg accattggtg gccgctatga ccgaagaaac cccgaacgaa
     2761 ccctttgacg aggtcgaata tcgccgccgt cagcgcagcc gcgcgaacct gatggcgtgg
     2821 atgctcggcg cattggcgat cctctttttc ttcatcacca tcgccaagat gcagattttc
     2881 tcatgacgtt ttttccggtc attgggcgca tgtccccgaa cgcgaagacc gcaagcctcg
     2941 cggcgttgat ggcgctcgcg atgaccggtc tcgggttcgc ggcggtgcct ctctacgatc
     3001 tcttctgccg cgtcaccggc ttcggcggta cgacgcagcg ttacgatccg gtcgccgcgg
     3061 cggccgagcc cgaaatattg agccagacgg tctcggtccg tttcgacgcc aatgtctcgc
     3121 cgaacctgcc gtggaaattc tatcccgagc atccgaccga tacggtgagc atcggcgcgc
     3181 gcgacatggc gatcttcatt gccgaaaata attcggcgca tcccgtcgtc gggaccgcca
     3241 gcttcaacgt cacacccgat caggcgggca aatattttac caagatccag tgcttctgct
     3301 tcacccagca acggctcgag ccggggcagc agatgcgcat gccggtgctg ttcttcgtcg
     3361 atcccaagat catggacgac cccgacgcgc gcgacgtgca ggaaatcacc ctcagctaca
     3421 ccttccaccc tgtagacgag ggtaaaaagg cgagctaagg ccgcgaacaa gagtctaaca
     3481 agaccggcag atgatgggga atcatcatgc tggggctgct aaaaacggga aacacgccat
     3541 gtccggtgcg aaacatcatg actaccacct cgtaaatccc agcgtctggc cgctcatcgg
     3601 ctcggtcgct gccctcgtga tgttcttcgg gctcgtcatg gcgatgcacg ccgactattt
     3661 cggcggcatc ggcaagtggg tgctcggcct cggcttcatg ggcgtgatcg ccaccttctt
     3721 cagctggtgg tcggacgtca tcaacgaagc ccacgccggc gaccacaccc cggtcgtcca
     3781 gctgcacctg cgctacggca tgatcctgtt catcgcgtcc gaagtcatgt tcttcgtcgg
     3841 ctggttctgg gcgtggttcg acttctcgct cttcccggtg ccgatcgaat atgccgaagg
     3901 cgcggtcacc tcgctgttcg gccaggacgg cgcggcggcg atcaccatgt ggccgccgaa
     3961 gggcatcgaa gtcatcgatc ccttctcgct gccgctgctc aacacgctga ttctgctctg
     4021 ctcgggtacg acgatcacct gggcgcacca tgcgctgatc cacggcgacc gcgaagggct
     4081 caagaagggc c
//
LOCUS       DATMSU010000009         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_123409,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000009 DATMSU010000000
VERSION     DATMSU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123409"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..635)
                     /locus_tag="VN034_00230"
     CDS             complement(159..635)
                     /locus_tag="VN034_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AHH domain-containing protein"
                     /protein_id="HWV59051.1"
                     /translation="MPGRGWHGAPAAQAGFQRHHLIPVSLMQRPQMAAMFEQLRIEGF
                     ALHHFDRNGLVLPACEPAALRSGHALHRGPHQGYSDVVAARVEMIRAHFALQAPADLR
                     GARRTAVMRLRLLQDTTRRALTDGHGGGFWLNRRDPMRLFFDRPYLDDAIERLFGG"
     gene            complement(635..1468)
                     /locus_tag="VN034_00235"
     CDS             complement(635..1468)
                     /locus_tag="VN034_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59052.1"
                     /translation="MDADDRDALILPVPPEPFALILGPAMRGAPDEAIETSLRHMGLQ
                     PERVRDAGAGPDARIFAPRFRVKFGAVSVLAGPAAGAALDLADPDHPSTSLLAASLPR
                     SWRESGDCWIFIPENRGSAAPPRAQTTRMREFFKMMVLMIDLFDASHIFWSPARLWSD
                     APQFRASIAEMLASGMPPVLHLVAFRRKDLGDGASMRTRGLALFAGQELEARIPDGWT
                     VAEMVRRLSRLALDMMLNGPVRTARTMRGLDAGEWIDLLPPMDGASQRSTVIVEFGRD
                     G"
     gene            complement(1585..3546)
                     /locus_tag="VN034_00240"
     CDS             complement(1585..3546)
                     /locus_tag="VN034_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HWV59053.1"
                     /translation="MTAESQQAGGGAAGAVEAGADTGHHGHGHEGDGKLKLAVGAIGV
                     VFGDIGTSPLYAFRETFAGHHPIEADRLHIYGVLSLVFWSMMLVVTFKYVLTIMKADN
                     KGEGGSLALLALISRSSGEKRWTWPIILLGVFATALFYGDSMITPAMSVLSATEGLSY
                     VNKGFEPYIVPIALAILIGLFAIQSRGTAKVGMLFGPIMLAYFTMLAILGIIHIVDHP
                     GIILETLNPVNALRFFYVDGFTAFIALGAVVLAVTGAEALYADMGHFGRGPIGLSWLT
                     FVLPALMLNYMGQGAMVLDAEMGPRGDLIADPFFQMMPDAWQIPVVILAILATIIASQ
                     AVISGAFSLTQQAIQLGFMPRLRVEHTSASAQGQIYIPMVNWGLMVMVILLVLGFGSS
                     SNLAAAYGIAVTGAMFIDTCLMSVVLFTLWKWPAWKALPILAVFFIVDIAYFGANLIK
                     VPDGGWVPLAIGLTIFTMLTTWSRGRKLMQQEMAEGAMPIPVFVKSAANSATRVPGTA
                     VFMTSSSDGVPHALLHNLKHNKVLHERIILLTIKIADVPYVRAENHCALDDLGQGFHR
                     LILNYGFMQPIDVPAALKRVTSCGGEFKMLETSFFLSRQTLIAASKPGMPIWREKLFA
                     WMLRNSESAMEYFRLPTNRVVELGSQVAI"
BASE COUNT          696 a         1273 c         1141 g          577 t
ORIGIN      
        1 gtcgggaccc gatccgcgcg aaaggcatca tggccagcgg cgcatgacaa ggccgcatca
       61 caggccggac acatggctgc accaaccagc aggtcgcatc acatccagaa accacttgcc
      121 aacggagggc cgtccacaca cggtcactcg ctgccgccct atccgccaaa caaccgctcg
      181 atcgcatcgt ccagataggg acgatcgaag aacaaccgca tcggatcgcg ccggttcagc
      241 cagaatcccc ctccatgccc gtcagtcagc gcccgccgtg tcgtgtcctg cagcaagcgc
      301 aggcgcatga ccgccgtgcg ccgcgcgcct cgcaggtctg ccggcgcctg caacgcgaaa
      361 tgcgccctga tcatctcgac ccgcgcggcg acgacgtcgc tatacccctg atgcggcccg
      421 cgatgcaggg catgacccga tcgcagcgcg gcgggctcgc acgccggcag caccaagccg
      481 ttgcggtcga aatgatgaag cgcgaaccct tcgatccgca attgctcgaa catcgccgcc
      541 atttgcggcc gctgcatcag gctgacgggg atcaggtggt ggcgctggaa cccggcctgc
      601 gccgccggcg ccccatgcca gccacgcccc ggcatcaacc gtcgcgaccg aattcgacga
      661 tcaccgtcga cctttggctc gcgccatcca tcggcggcag caggtcgatc cactcgcccg
      721 catccagccc gcgcatcgtc cgcgcggtcc gaaccgggcc gttgagcatc atgtcgagcg
      781 cgagccgcga caatcgccgc accatttcgg cgaccgtcca gccatcgggt atacgcgcct
      841 cgagttcctg cccggcgaaa agcgccagcc cgcgcgttcg catgctcgcg ccatcgccca
      901 gatccttgcg ccggaaggcg acgagatgaa gcaccggcgg catcccgctc gccagcattt
      961 cggcgatcga ggcgcggaac tgcggcgcat cggaccagag ccgcgcgggc gaccagaaga
     1021 tgtggctggc gtcgaacagg tcgatcatca acaccatcat cttgaaaaat tcgcgcatcc
     1081 gcgtcgtctg cgcccggggc ggcgctgcgg acccgcgatt ttcgggaatg aagatccagc
     1141 aatcgccgct ctcgcgccag ctgcgcggca ggctggcggc gagcaggctc gtcgacggat
     1201 gatcgggatc ggcgaggtcg agcgccgcgc ccgccgcggg gccggcgagc accgataccg
     1261 ccccgaattt tacgcgaaag cggggggcga agatccgcgc atcggggccc gcaccggcat
     1321 cgcggacccg ctcgggctgc aaccccatat ggcggagcga tgtttcgatc gcctcgtccg
     1381 gtgcgccgcg catcgcgggc cccagaatca gcgcgaaggg ttccggcgga accggcagga
     1441 tcaacgcatc gcggtcatcg gcatccatct gttgcgcgcc cttcatgggt tcgccgcttg
     1501 catcgcaaga ccgatccgaa tgttcaaacg cttatcgacg catgtcggaa agttaatcgg
     1561 gcgccccatg ctccgccggt cgcgtcagat cgcgacctgg ctgcccagct cgacgacgcg
     1621 gttggtgggc aggcggaaat attccatcgc gctttccgag ttgcgcagca tccacgcgaa
     1681 cagcttttcg cgccagatcg gcatgcccgg cttgctcgcc gcgatcaacg tctggcgcga
     1741 gaggaaaaag ctcgtctcga gcatcttgaa ctcgccgccg cagctcgtca cgcgtttgag
     1801 cgcggcgggc acgtcgatcg gctgcatgaa gccgtaattc aggatcagcc ggtggaaccc
     1861 ctgccccaga tcgtcgagcg cgcagtgatt ttccgcgcgt acatagggga cgtcggcgat
     1921 cttgatcgtc agcaggatga tgcgctcgtg cagcaccttg ttgtgcttga ggttatggag
     1981 cagcgcgtgc ggcaccccgt cgctgctcga cgtcatgaac accgcggtgc ccggaacgcg
     2041 cgtcgcgctg ttcgccgccg acttcacgaa caccgggatc ggcatcgccc cctcggccat
     2101 ttcctgctgc atcagcttgc gtccgcgcga ccaggtcgtg agcatcgtga agatcgtcag
     2161 gccgatcgcc agcggcaccc agccgccgtc gggcaccttg atcaggttcg cgccgaaata
     2221 ggcgatgtcg acgatgaaga agaccgctaa gatcggcagc gccttccacg ccggccactt
     2281 ccacaaggtg aagagcacga cgctcatcag gcaggtgtcg atgaacatcg cgccggtcac
     2341 cgcgatcccg taagcggcgg cgaggttgct cgacgatccg aagccgagga cgagcaggat
     2401 caccatcacc atcagccccc agttgaccat cgggatatag atctgcccct gcgccgatgc
     2461 gctcgtatgc tcgacgcgca gccgcggcat gaagcccaat tggatcgcct gctgcgtcag
     2521 cgagaaggcc cccgaaatca ccgcctggct cgcgatgatc gtcgcaagga tcgcgaggat
     2581 gacgaccggt atctgccatg cgtcgggcat catctggaaa aagggatcgg cgatcaggtc
     2641 gccgcgcggc cccatctccg catcgagcac catcgcgccc tgccccatat agttaagcat
     2701 cagcgccggc agcacgaagg tcagccagct cagcccgatc ggcccgcgtc cgaaatgccc
     2761 catatcggca taaagcgcct cggcccccgt caccgcgagc accaccgcgc ccaatgcgat
     2821 gaaggcggtg aagccgtcga catagaagaa gcgcagcgcg ttcacggggt tgagcgtttc
     2881 gaggatgatc cccggatggt cgacgatatg gatgatcccg aggatcgcga gcatcgtgaa
     2941 ataggcgagc atgatcggcc cgaacagcat cccgaccttc gcggtcccgc gcgactggat
     3001 cgcgaacagc ccgatcagga tcgcgagcgc gatcggcacg atatagggct cgaacccctt
     3061 gttgacgtag ctcagcccct cggtcgccga gagcaccgac attgccgggg tgatcatgct
     3121 gtcgccataa aaaagcgcgg tcgcgaacac gccgagcagg atgatcggcc aggtccagcg
     3181 cttctcgccc gacgagcggc tgatcagcgc gagcagcgcc aaactgcccc cctcgccctt
     3241 gttgtcggct ttcatgatcg tcagcacata tttgaaggtg acgacgagca tcatcgacca
     3301 gaagacgagg ctcaagacgc catagatgtg cagccggtcg gcctcgatcg gatggtgccc
     3361 ggcaaaggtt tcgcgaaacg cgtagagcgg gctggtgccg atgtcgccga acacgacccc
     3421 gatcgcgccg acggccagct tcagcttgcc atcgccctca tgcccatggc cgtgatggcc
     3481 ggtgtcggcg ccagcttcga cggcgcccgc ggcgcccccg cccgcctgtt gcgactcagc
     3541 agtcatcgcg cgcctttaga cgagcctgcg cctttggaac aggtttcaat ttgggacgcc
     3601 cccggccggc acatcggtcg gggtttcgcc agaggtttca gaaggaagct gcggccccag
     3661 cgcctgccgg agctgcacca gccgcac
//
LOCUS       DATMSU010000010         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_19191, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000010 DATMSU010000000
VERSION     DATMSU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19191"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="VN034_00245"
     CDS             complement(<1..454)
                     /locus_tag="VN034_00245"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HWV59054.1"
                     /translation="MATAAKSDLWVRLASAIILFAIAGTALWFGGIAFGLLLLIGGAL
                     VLVEWFQLVKAMTLGGGAKTALHILGPILVLGAMAGLWFVRDHLGMTAALWVFGMVWA
                     TDIGAYFAGRAFGGARLAPKISPSKTWSGLFGGMVAALIASATIGDRAG"
     gene            complement(459..1145)
                     /gene="uppS"
                     /locus_tag="VN034_00250"
     CDS             complement(459..1145)
                     /gene="uppS"
                     /locus_tag="VN034_00250"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWV59055.1"
                     /translation="MTETTHGARHVAIIMDGNGRWAKKRLLPRVAGHKAGVEAVRTIV
                     RAAGDLGIEAMTLYAFSSENWKRPEEEVSDLMGLMKRFILSDLDEFAANDVKLKVIGN
                     WRALAPDVVALIENALERTSGNKRTTLAVALNYGAQDELVRAATAAAAGGKITAEAIE
                     ASLDTADMPPLDLLIRTSGEVRLSNFLLWQSAYAELYFTDILWPDFKPADLKAALDHF
                     ARRDRRYGGL"
     gene            complement(1266..1820)
                     /gene="frr"
                     /locus_tag="VN034_00255"
     CDS             complement(1266..1820)
                     /gene="frr"
                     /locus_tag="VN034_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWV59056.1"
                     /translation="MAKYDKADLERRMHGAVESLKHDLAGLRTGRAHTALLDPVTVEV
                     YGSHMPLNQVASVSAPEPRMLSVQVWDKSNVGPVDKAIRSAGLGLNPIVDGQMLRLPI
                     PEMTQERRKELSKLAGQYAEKARVAVRNVRRDGMDNLKADENKKEISEDERKRHETEV
                     QKLTDATIAELDAAATAKEKEILG"
     gene            complement(1844..2569)
                     /gene="pyrH"
                     /locus_tag="VN034_00260"
     CDS             complement(1844..2569)
                     /gene="pyrH"
                     /locus_tag="VN034_00260"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWV59057.1"
                     /translation="MALPGMKRILLKLSGEALMGSSPFGIDPETVASMAAEVKEAKDR
                     GLEICLVIGGGNIFRGMAGAAKGMDRAQADYMGMLATVMNALAMQNALEQLGVETRVQ
                     SAIEMDKVCEPVIRRRAERHMEKGRVVIFAAGVGAPYFTTDSGAALRAAEMKCDALLK
                     GTSVDGVYNADPKKDASAVRYDTLSYDRVLADNLKVMDASAVALCRDNHIPIVVFNIR
                     EPGNLARVLAGEGVSTVVTDAAG"
     gene            complement(2681..>3466)
                     /gene="tsf"
                     /locus_tag="VN034_00265"
     CDS             complement(2681..>3466)
                     /gene="tsf"
                     /locus_tag="VN034_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWV59058.1"
                     /translation="AAAKKAGRVAAEGLVGVATDGNRGAMVEVNSETDFVAKNEQFQG
                     FVRDVTQVALAGSITDIDALNAAAYPGAATVAEQLTQNIATIGENQSLRRSAIVTVNS
                     GVVTGYVHNAAAPGMGKIGVLVALESTAGADVLEPLGKQLAMHVAAANPLALNGDDLD
                     ADLVARERAIAEEKAAQSGKPAEIVGKMVDGAIAKYRKENALLSQLFVMDGKTPVAEV
                     VAAAGKDVGATITLKGFVRFQLGEGIEKEESDFAAEVAAAAGV"
BASE COUNT          523 a         1169 c         1127 g          647 t
ORIGIN      
        1 tgcccgcacg gtcgccgatc gtcgcgctgg cgatcagcgc cgcgaccatg ccgccgaaca
       61 gccccgacca ggtcttcgac gggctgattt tcggcgcaag gcgcgcgccg ccgaaggcgc
      121 ggccggcgaa ataggcgccg atgtcggtcg cccacaccat gccgaagacc cacagcgccg
      181 cggtcatgcc gagatggtcg cggacgaacc agagccccgc catcgcgccg agcacgagga
      241 tcgggccgag gatatggagc gcggtcttcg cgccgccgcc gagcgtcatc gccttcacga
      301 gctggaacca ttcgacgagg acgagcgccc cgccgatgag cagcagcaga ccgaaggcga
      361 tcccgccgaa ccacagcgcg gtgcccgcga tcgcgaacag gatgatcgcc gacgcgagcc
      421 gcacccagag atccgatttc gccgctgtcg ccatgccctt aaagtccccc gtaacggcga
      481 tcgcggcgcg cgaaatggtc gagcgccgct ttgagatcgg ccggtttgaa gtccggccac
      541 aagatgtcgg tgaaataaag ctcggcatag gccgattgcc acagcaggaa gttcgacagc
      601 cggacttcgc ccgaggtgcg gatgagcagg tcgagcggcg gcatgtcggc ggtgtcgagg
      661 ctcgcctcga tcgcctcggc ggtgattttg ccccccgcgg cggcggcggt cgccgcacgc
      721 accagttcgt cctgcgcgcc atagttgagc gcgaccgcga gcgtcgtgcg cttgttgccc
      781 gacgtgcgtt cgagcgcatt ttcgatcagc gcgacgacgt cgggcgcgag cgcgcgccaa
      841 ttgccgatca ccttgagctt cacgtcgttc gcggcgaatt cgtcgaggtc ggacaggatg
      901 aagcgcttca tcagccccat cagatcgctg acctcttcct cgggccgctt ccagttttcg
      961 gaactgaagg catagagcgt catcgcctcg atgccgaggt cgcccgccgc gcgcacgata
     1021 gtgcgcaccg cctcgacccc cgccttgtgt ccggcgacgc ggggcagcag gcgcttcttc
     1081 gcccagcggc cgttgccgtc cataatgatg gcgacatggc gcgcgccgtg agtggtttct
     1141 gtcatcgtct atccgtcatc ccagcgaaag ctgggatcgc tttcgaccaa gcgccgcgct
     1201 tgcacgcctg cggtcccagc tttcgctggg acgacgaaaa ttgtgccatg cggccattcg
     1261 gtccactagc ccaggatttc cttttccttg gcggtcgcgg cggcgtcgag ctcggcaatc
     1321 gtcgcgtcag tcagcttctg cacctcggtt tcgtggcgct tgcgctcgtc ctcgctgatt
     1381 tccttcttat tctcgtcggc tttcagattg tccatgccgt cgcggcggac gttgcggacc
     1441 gcgacgcgcg ccttttcggc atattggccg gcgagcttcg acaattcctt gcggcgctcc
     1501 tgcgtcattt cggggatcgg caggcgcagc atctggccgt cgacgatcgg attgaggccg
     1561 aggccggccg agcggatcgc cttgtcgacc gggccgacgt tcgacttgtc ccacacctgc
     1621 accgacagca tgcgcggttc gggcgcgctg accgaggcga cctggttcag cggcatatgg
     1681 ctgccataga cctcgaccgt caccgggtcg agcagcgcgg tgtgcgcgcg gccggtgcgc
     1741 aggcccgcaa ggtcgtgctt taaggattcg accgcgccgt gcatccggcg ttcgaggtcg
     1801 gccttgtcat atttcgccat ctgtctttcc tctcttgatt ctgtcagccc gcggcgtcgg
     1861 tgacgacggt ggaaacaccc tcgcccgcca gcacgcgcgc cagattgccg ggctcgcgga
     1921 tgttgaacac gacgatcggg atatgattgt cgcggcacag cgccaccgcg ctcgcgtcca
     1981 tcaccttcag attgtcggcg agcacacggt cgtagctgag cgtgtcataa cgcaccgccg
     2041 acgcgtcctt cttgggatcg gcgttgtaga cgccatcgac gctggtgccc ttgagcaacg
     2101 catcgcattt catttcggcg gcgcgcaatg ccgcaccgct gtcggtcgta aaatagggtg
     2161 ccccgacgcc cgcggcgaag atgaccacgc ggcccttttc catatggcgc tcggcgcggc
     2221 ggcggatgac gggctcgcac accttgtcca tctcgatcgc cgactggacg cgcgtttcga
     2281 cgccgagctg ctcgagcgca ttctgcatcg cgagcgcgtt catcacagtg gcgagcatgc
     2341 ccatatagtc ggcctgcgcg cggtccatgc ccttggccgc gcccgccatg ccgcggaaaa
     2401 tgttgccgcc accgatgacg aggcagattt cgagcccgcg atccttggct tccttgacct
     2461 cggccgccat gctcgcgacg gtttcggggt cgatgccgaa cgggctggaa cccatcagcg
     2521 cctcgcccga cagcttgagc aaaatgcgtt tcatgccggg caatgccatg tgcgggggaa
     2581 cctcttgcaa actctcaata cgcgaatggc gcgcacccta gtgaaggcgc gcgccattgc
     2641 caagcggact gccgcgacgc ggcaatccgt atcttctctc ttagacgcca gcggcggccg
     2701 cgacttccgc cgcgaagtcg ctttcttcct tctcgatgcc ttcgccgagc tggaagcgga
     2761 cgaagccctt cagggtgatc gtcgcgccga cgtccttgcc ggcggcggcg acgacttcgg
     2821 cgaccggggt cttgccgtcc atcacgaaga gctgcgacag cagcgcgttt tccttgcgat
     2881 atttggcgat cgcgccgtcg accatcttgc cgacgatttc ggcgggcttg cccgactggg
     2941 ccgctttttc ttccgcgatc gcgcgttcgc gggcgacgag atcggcgtcg aggtcgtcgc
     3001 cgttcagcgc cagcgggttc gcggcggcga cgtgcatcgc gagctgcttg ccgagcggtt
     3061 cgagaacgtc ggcgccggcg gtcgattcga gcgcgacgag cacgccgatc ttgcccatgc
     3121 cgggcgcggc ggcgttgtgg acatagcccg tgacgacgcc cgagttcacc gtgacgatcg
     3181 cgctgcggcg cagcgactgg ttctcaccga tcgtcgcgat gttctgcgtc agctgttcgg
     3241 cgacggtggc ggcgcccgga taggccgcgg cgttcagcgc gtcgatgtcg gtgatgctgc
     3301 cggcgagcgc gacctgcgtc acgtcgcgga cgaagccctg aaactgctcg ttcttcgcga
     3361 cgaagtcggt ttcgctgttc acttcgacca tcgcgccgcg gttgccgtcg gtggcgacgc
     3421 cgacgaggcc ttccgcggcg acgcggccgg ccttcttggc ggcggc
//
LOCUS       DATMSU010000011         5986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_66582, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000011 DATMSU010000000
VERSION     DATMSU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66582"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /gene="thrC"
                     /locus_tag="VN034_00270"
     CDS             complement(<1..1144)
                     /gene="thrC"
                     /locus_tag="VN034_00270"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWV59059.1"
                     /translation="MDYISTRGSAPTLDFRAATLAGLASDGGLYVPAKWPQLSIDEIR
                     ALAGLDYVETAVRVMAPFVAGALSEDELRELCRAAYGRFSHDAVTPLVQLDHRHWLLE
                     LFHGPTLAFKDVALQLLGQLFEKFLSGGDTDITIVGATSGDTGSAAIEAVAGREHIRI
                     FMLHPEGRVSDVQRRQMTTVLAPNVHNIAIDGSFDDAQAMVKRLFGDAEARGQVTLSA
                     VNSINWARLMAQIVYYFYAAVRLGGPDRPVAFAVPTGNFGDVFAGYVAAQMGLPVAKL
                     VVATNVNDILHRALTSGDYSAGTVTPTATPSMDIQVSSNFERLLFDLAGRDGVAITAM
                     MGEFDANRAMTIPADMLAGARDLFSSASIDGDAMALALRWAQERGGQ"
     gene            complement(1226..1885)
                     /locus_tag="VN034_00275"
     CDS             complement(1226..1885)
                     /locus_tag="VN034_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWV59060.1"
                     /translation="MTDPAGPAETPAPARRWPLIPTLLVLAAVGVMIALGVWQLQRKA
                     DKEALIALYQRNMAMSSLVTYPELPPVPDTMLYRKSSVVCLEPVRWDPRSGTDRKGRS
                     GIRMIADCRTGAEGPGVLVDVGIGDDFEPPEWTGGTVQGTIVPGPEQPTVIARMTGKA
                     VPARAMLVADAPVANLRASGVPSADDTPNNHLAYAVQWFLFAAAALVIYILAVRRRLR
                     P"
     gene            complement(1878..2273)
                     /locus_tag="VN034_00280"
     CDS             complement(1878..2273)
                     /locus_tag="VN034_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWV59061.1"
                     /translation="MSADNQPQKGQPPVWRAALFGLCPRCGAQTLFEGPVKFAPRCRV
                     CALDFEGYNVGDGPAAFLTLIIGALLIAIALTLDALVRPPFWVHVLLWVPLTAAAVVY
                     GLRVGKGALLASEHQRQAGEGRKVEDEND"
     gene            complement(2275..3150)
                     /locus_tag="VN034_00285"
     CDS             complement(2275..3150)
                     /locus_tag="VN034_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWV59062.1"
                     /translation="MSGAKHHDYHLVNPSVWPLIGSVAALVMFFGLVMAMHADHFGGV
                     GKWVLGLGFMGVIATFFSWWSDVINEAHAGDHTPVVQLHLRYGMILFIASEVMFFVGW
                     FWAWFDFSLFPVPIEYAEGAVTSLFGQDGADAITMWPPKGIEVIDAFSLPLLNTLILL
                     CSGTTITWAHHSLIHGDREGLKKGLWLTIILGAVFSAIQAYEYAHAPFGFGQSNYSSA
                     FYMATGFHGFHVLVGTIFLIVCLVRTYKGHFTPQQHFGFEAAAWYWHFVDVVWLFLFI
                     IVYVWGGWGAPVAAH"
     gene            complement(3233..3793)
                     /locus_tag="VN034_00290"
     CDS             complement(3233..3793)
                     /locus_tag="VN034_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWV59063.1"
                     /translation="MMGRLSPNAKTASLAALMALAMTGLGFAAVPLYDLFCRVTGFGG
                     TTQRYDPVAAAAEPQILSDTISVRFDANVSPNLPWKFYPEHPTDTVSVGARDMAIFIA
                     ENNSSQPVVGTASFNVTPDQAGKYFTKIQCFCFTQQRLEPGQQMRMPVLFFVDPKIKD
                     DPDARDVQEITLSYTFHPVDGDKNAS"
     gene            complement(3790..3954)
                     /locus_tag="VN034_00295"
     CDS             complement(3790..3954)
                     /locus_tag="VN034_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59064.1"
                     /translation="MTDEPNLEPFDSKNFDEAEYRRRQRSRANLMAWMLGALAILFFF
                     ITIAKMQIFA"
     gene            complement(3951..4877)
                     /locus_tag="VN034_00300"
     CDS             complement(3951..4877)
                     /locus_tag="VN034_00300"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWV59065.1"
                     /translation="MAGVTMAQSLTHGETALPADWRDLFALTKPRVMSLVVFTALCGL
                     LAAPGEVHPVLAFSSILAIALGAGASGALNQWYEAGLDAKMKRTAGRPLPAGRLDPQT
                     ALQFGVGLAAFSLFLMLFASNWQATLLLAVSILFYVFVYTMWLKPRTPQNIVIGGAAG
                     AFPPLIGWVAATGSIAPLPVLLFLLIFLWTPPHFWALALFVRSDYAAAGIPMMPVVAG
                     EKSTRRQILFYAIIMAIGAIAPWPLGYTGALYGWTAVVLSAVFVLLSVQVGMRTTGEG
                     DLMQPEKRLFAYSIAYLFILFGAVVADHWWPL"
     gene            complement(4922..>5986)
                     /locus_tag="VN034_00305"
     CDS             complement(4922..>5986)
                     /locus_tag="VN034_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWV59066.1"
                     /translation="GAINFITTIFNMRAPGMTLHKMPLFVWSVLVTAFLLLLALPVLA
                     AAITMLLTDRNFGTTFYDAAGGGDPVLYQHLFWFFGHPEVYIMILPGFGIVSQIISTF
                     SRKPVFGYLGMAYAMVAIGVVGFIVWAHHMFTVGMSVNLKMYFTAATMVIAVPTGIKI
                     FSWIATMWGGSMSFKTPMVWSLGFIFMFTVGGVTGVVLANGGVDTNLHDTYYVVAHFH
                     YVLSLGAVFSLFAGFYYWFPKMSGRMYSEFLGQLHFWIFFIGVNVLFFPQHFLGQQGM
                     PRRYPDYAEAYAFWHLISSYGYVIMGVGVLIFFANILYSLFAGKRAADNPWGEGATTL
                     EWTLSSPPPFHQFNELPRIA"
BASE COUNT         1207 a         1895 c         1961 g          923 t
ORIGIN      
        1 cctgcccgcc gcgctcctgc gcccagcgca gcgcgagcgc catcgcgtcg ccgtcgatgc
       61 tggcgctcga aaacagatcg cgcgcgcccg cgagcatgtc ggcgggaatc gtcatcgcgc
      121 ggttcgcgtc gaactcgccc atcatcgcgg tgatcgcgac gccatcgcgg cccgcgaggt
      181 cgaagagcag ccgttcgaaa ttgctgctga cctggatgtc catgctcggc gtcgcggtcg
      241 gcgtgaccgt gcccgcgcta tagtcgccgc tggtcagcgc gcggtggagg atatcgttga
      301 cgttggtcgc gacgacgagc tttgccacgg gcaagcccat ctgcgccgcg acatagcccg
      361 cgaacacgtc gccgaaattg cccgtcggca cggcgaacgc caccggacgg tcgggtccgc
      421 cgagacgaac ggcggcgtag aaataataga cgatctgcgc catcagccgc gcccagttga
      481 tgctgttcac cgccgacagc gtcacttgcc cgcgcgcttc tgcgtcgccg aacagccgct
      541 tcaccatcgc ctgcgcatcg tcgaagctgc cgtcgatcgc gatattgtga acgttgggcg
      601 cgagcaccgt cgtcatctgg cgcctctgga cgtcgctcac ccgtccttcg gggtggagca
      661 tgaagatgcg gatatgttcg cggcccgcga ccgcctcgat cgccgccgat ccagtatcgc
      721 ccgaggtcgc gccgacgatc gtgatgtcgg tatcgcctcc gctaaggaat ttctcgaaca
      781 gctggccgag cagctgcagc gcgacatcct tgaacgcaag cgtcggccca tggaacagtt
      841 cgagcagcca gtgacgatgg tcgagctgga cgagcggcgt caccgcgtcg tggctgaagc
      901 ggccataagc ggcgcggcaa agctcccgca gttcatcctc gctcagcgcg cccgcaacga
      961 acggcgccat cacccgcacc gcggtctcga cataatcgag cccggcgagc gcgcgaattt
     1021 cgtcaatgct caactgcggc catttggccg gaacataaag cccgccgtcg ctggcgaggc
     1081 ccgccagcgt ggcggcgcga aagtcgaggg tcggcgcaga gccgcgggtg ctgatatagt
     1141 ccatgacgca gatgcgccta gagcggtttc gagcgaaatg gaacatttcg cggctcggaa
     1201 atcgcggaaa aactttgggg gcggctcaag gtcgcaagcg gcgacgaacg gccaatatat
     1261 agatgacgag cgccgcggcg gcgaacagga accattgcac cgcataggcg agatggttgt
     1321 tcggcgtatc gtcggccgag ggcaccccgc tcgcgcgcaa attcgcgacc ggcgcatcgg
     1381 cgaccagcat tgcgcgcgcg ggcaccgcct tgcccgtcat gcgcgcgatc accgtcggct
     1441 gctcggggcc cgggactatc gtcccctgca ccgtgccgcc ggtccattcg gggggctcga
     1501 aatcgtcgcc gatcccgacg tcgacgagca cgccgggccc ttcggctccc gtccggcaat
     1561 cggcgatcat gcggatgccc gaccggccct tgcggtcggt gccgctgcgc ggatcccagc
     1621 gcacgggttc gaggcagacg acgctgctct tgcgatagag catcgtatcg ggaaccggcg
     1681 gcagctcggg ataggtcacc agtgatgaca tcgccatgtt gcgctggtaa agcgcgatca
     1741 gcgcctcctt gtcggccttg cgctggagct gccagacgcc gagcgcgatc atcaccccca
     1801 ccgccgcgag gacgagcagg gtcgggatca gcggccagcg ccgcgcaggt gcgggcgttt
     1861 ccgcgggtcc ggcgggatca gtcattttcg tcctccacct tgcggccctc gccggcctgg
     1921 cgctgatgct cgctcgcgag caacgcgccc ttgcccacgc gcaggccgta aacgaccgcg
     1981 gcggcggtca gcggcaccca caagagcaca tgcacccaga agggcggccg gaccagagca
     2041 tcaagcgtca gtgcgatcgc gatcaacagc gcaccgatga tcagcgtcag gaaggccgcg
     2101 ggcccgtcgc cgacattata gccctcgaaa tcgagcgcgc agacgcggca gcgcggcgcg
     2161 aacttcaccg gcccctcgaa caatgtctgc gcgccgcacc gcggacagag gccaaaaagg
     2221 gcagcgcgcc agaccggcgg ctgccccttt tgcggttgat tgtcggcgga caatttagtg
     2281 cgcggcgacc ggcgcgcccc agccgcccca gacatagacg atgatgaaga ggaacagcca
     2341 cacgacgtcg acgaagtgcc agtaccacgc cgcggcttcg aaaccgaaat gctgctgcgg
     2401 ggtgaagtgg cccttatagg tacgcacgag gcagacgatc aggaagatcg tgccgaccag
     2461 aacgtggaag ccgtggaagc cggtcgccat atagaaggcc gagctgtaat tgctctgccc
     2521 gaaaccgaac ggcgcatggg catattcata ggcctggatc gccgagaaga cggcgccgag
     2581 gatgatcgtc agccacaggc cttttttcag gccctcgcgg tcgccgtgga tcagcgaatg
     2641 gtgcgcccag gtgatcgtcg tgcccgagca gagcaggatc agcgtgttga gcaagggcag
     2701 cgagaaggcg tcgatgactt cgatgccttt cggcggccac atggtgatcg catcggcgcc
     2761 gtcctggccg aacagcgacg tcaccgcgcc ttcggcatat tcgatcggaa ccggaaagag
     2821 cgagaagtcg aaccaagccc agaaccagcc gacgaagaac atcacttcgg acgcgatgaa
     2881 caggatcatg ccgtagcgca ggtggagctg gacgaccggg gtgtggtcgc cggcatgcgc
     2941 ttcgttgatg acgtccgacc accagctgaa gaaggtcgcg atcacgccca tgaagccaag
     3001 gccgagcacc catttgccga cgccgccgaa atggtcggcg tgcatcgcca tgacgagccc
     3061 gaagaacatc acgagcgcag cgaccgagcc gatcagcggc cagacgctgg gatttacgag
     3121 gtgatagtca tgatgtttgg caccggacat ggcgtgtttc ccgttttttt gcagccccag
     3181 catgacgatt ccccatcgtc cgccggtctt tgattgactc tcggatgcgg ccttagctcg
     3241 cgtttttatc cccgtctaca gggtggaagg tgtagctgag ggtgatttcc tgcacgtcgc
     3301 gcgcgtcggg gtcgtccttg atcttcggat cgacgaagaa caggaccggc atgcgcattt
     3361 gctgccccgg ctccagccgc tgctgggtga agcagaaaca ctggatcttg gtgaaatatt
     3421 tgcccgcttg gtcgggggtg acgttgaagc tcgcggtgcc gaccaccggc tgcgacgaat
     3481 tattctcggc gatgaagatc gccatgtcgc gcgcgccgac gctgacggtg tcggtcggat
     3541 gctcgggata gaatttccac ggcaggttcg gcgagacatt ggcgtcgaag cgcaccgaga
     3601 tggtgtccga cagtatctgc ggctcggccg cggcggcgac ggggtcgtac cgctgcgtcg
     3661 tgccgccgaa accggtcacc cggcagaaaa gatcgtagag cggcaccgcc gcgaacccga
     3721 gcccggtcat cgcgagcgcc atcagcgcgg cgaggcttgc ggtcttcgcg ttcggggaca
     3781 gccggcccat catgcgaaaa tctgcatctt ggcgatcgtg atgaagaaaa agaggatcgc
     3841 gagcgcgccg agcatccacg ccatcagatt ggcgcggctg cgctggcggc gccgatattc
     3901 ggcctcatca aaatttttgg agtcgaaggg ctcaagattg ggttcgtcgg tcatagcggc
     3961 caccaatggt cggcgacgac agcgccgaac aggatgaaaa gataggcgat cgaataagcg
     4021 aacaggcgct tttcgggctg catcaggtcg ccctcgcccg tcgtgcgcat gccgacctgg
     4081 accgacagca ggacaaacac ggccgaaagc accaccgcgg tccagccata gagcgcgccg
     4141 gtgtagccga gcggccacgg cgcgatcgcg ccgatcgcca tgatgatcgc atagaacagg
     4201 atctgccgcc gcgtcgactt ttcgcccgca acaacgggca tcatcggaat gcccgcggcg
     4261 gcatagtccg accgcacgaa cagcgcgagc gcccagaaat gcggcggggt ccacaggaaa
     4321 atgagcagga agagcagcac cggcagcggc gcaatgcttc cggtcgcggc gacccagccg
     4381 atcagcggcg gaaaggcgcc cgccgcgccg ccgataacaa tattctgcgg cgtccgcggc
     4441 ttgagccaca tcgtatagac gaagacgtag aacaggatcg agacggcgag cagcagcgtc
     4501 gcctgccagt tcgacgcgaa cagcatcagg aacagcgaaa aggccgccag cccgacgccg
     4561 aactgcaacg ccgtctgcgg gtcgagacgc ccggcgggaa gcgggcggcc cgcggtccgc
     4621 ttcatcttgg cgtcgagccc cgcctcatac cattggttga gcgcgcccga cgcccctgcc
     4681 cccagcgcga tcgcgaggat cgacgagaag gcaagcaccg gatgcacctc gcccggcgcc
     4741 gcgagcagcc cgcacagcgc ggtgaacacc accagcgaca tgacgcgcgg tttggtcagc
     4801 gcgaacaggt cgcgccaatc ggcgggtaag gccgtttcgc cgtgggtcag gctctgcgcc
     4861 atggtcactc cggccatcag ggaaacgccc cctgccgatt atcagcaggg ggcataaccg
     4921 atcaggcgat acgcggcagt tcgttgaact ggtggaacgg cggcgggctc gacagcgtcc
     4981 attcgagcgt cgtcgcacct tcaccccacg gattgtcggc agcgcgcttg cccgcgaaca
     5041 gcgagtagag gatgttcgcg aagaagatca gcacgccaac gcccatgatc acgtaaccat
     5101 aggacgagat caggtgccag aaagcatagg cttccgcata gtcggggtaa cggcgcggca
     5161 tgccctgctg acccaggaaa tgctggggga agaacaggac gttcacgccg atgaagaaga
     5221 tccagaagtg cagctggccc aggaactcgc tgtacatccg gcccgacatc ttggggaacc
     5281 agtaatagaa gccggcaaag agcgagaaga ccgcgcccag cgacagcaca tagtggaagt
     5341 gcgcgacgac ataataggtg tcgtgcaggt tcgtatcgac gccgccgttg gcgaggacga
     5401 cgccggtgac gccgccgacg gtgaacatga agatgaagcc caaggaccag accatcgggg
     5461 tcttgaagct catcgaaccg ccccacatgg tcgcgatcca gctgaatatc ttgatccccg
     5521 tcgggaccgc gatcaccatc gtcgccgcgg tgaaatacat cttgaggttc acgctcatgc
     5581 cgaccgtgaa catgtggtgc gcccacacga tgaagccgac gacgccgatc gcgaccatgg
     5641 cataggccat gccgaggtag ccgaagaccg gcttgcggct gaaggtcgag atgatctggc
     5701 tgacgatgcc gaagcccggc aggatcatga tgtacacttc ggggtggccg aagaaccaga
     5761 agagatgctg gtagaggacg ggatcgccgc ccccggccgc atcatagaag gtggtgccga
     5821 agttgcggtc ggtcagcagc atcgtgatcg ccgcggcgag caccggcagc gcgagcagca
     5881 gcaggaaggc ggtgacgagc accgaccaca cgaacagcgg catcttgtgc agcgtcatgc
     5941 ccggcgcgcg catgttgaag atggtggtga tgaagttgat cgcacc
//
LOCUS       DATMSU010000012         4726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_95017, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000012 DATMSU010000000
VERSION     DATMSU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95017"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VN034_00310"
     CDS             <1..388
                     /locus_tag="VN034_00310"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWV59067.1"
                     /translation="ARTIRPALARGAWVLCDRFVDSSRAYQGGGGGITDADLLALHRF
                     GSEGLLPDRTFLLTVSPDEAARRLTERGGSDRMGNKPFDYQARLAARFVEMAAAETGR
                     WRIVDADRRAEEVTAAILADLAEWLP"
     gene            385..1365
                     /locus_tag="VN034_00315"
     CDS             385..1365
                     /locus_tag="VN034_00315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HWV59068.1"
                     /translation="MIGHQEAEKAFLEAWQGGRVHHAWLLAGPQGMGKGAFAERVARF
                     LVTHGRGGEGQTTTLDDRGDDAAARLVDAGNHPEVLRLARQPKDKAKELARNITIEQV
                     RQMIRRLHLSLSLGEWRVIIVDAVDDLETDGANALLKTLEEPPAKTLFLLVSHSPGRL
                     LPTIRSRCRTLRFQPVARDIMTTWLHDLRPMIEMEEVRAIVAASGGVPGKALALIDSD
                     VAAMEKKLLAIAANGDPENKLREALAREVGGTSNRARLELVIDIVPGLLSRIARERPV
                     SEIAPILAQWDRVQRTVRDAIRGSYDGAMVGFEIGNCLAELAPRHDRAAR"
     gene            1404..2981
                     /gene="metG"
                     /locus_tag="VN034_00320"
     CDS             1404..2981
                     /gene="metG"
                     /locus_tag="VN034_00320"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWV59069.1"
                     /translation="MSENNEPFYITTAISYPNGRPHIGHAYEAIATDVMARFQRARGR
                     DVRLVTGTDEHGLKMFQTARDQGRATIDLADEMSGYFREMYAKLNISYDNFMRTSDAA
                     HHAASQAIWKAMEAKGDLYLDRYEGWYSVRDEAFYDESELSDGEGGVRLSPQGTPVEW
                     TVEESWFFRLSSYQDRLLALYNEQPDFIRPESRRNEVLRFVEGGLKDLSVSRTSFDWG
                     VPVPGSPGHVMYVWVDALTTYLSALGYPDPDSDFGTFWPANIHMIGKDIVRFHTVYWP
                     AFLMSANLPLPQQVFGHGFLLNRGEKMSKSLGNVVDPMALADRFGVDALRYYLLREVS
                     FGQDGSYSAEAIVRTANADLANSFGNLAQRSLSMIFKNLDGRLSGDYAPAQDDIDLLA
                     DVANMAAVRLPHEFDQLAFSVGIEDWLRAVFACNQYVDTQAPWALRKSDPERMRAVLM
                     TLFQAVRTLAIAIRPVVPAAADKLLDQMGIAEDARDFAALSDTDWFTKLVASGFAVSQ
                     PVPIFPRLELPEGEGEA"
     gene            2981..3757
                     /locus_tag="VN034_00325"
     CDS             2981..3757
                     /locus_tag="VN034_00325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWV59070.1"
                     /translation="MLVDSHCHLNYKGLAEQQGEVLARARARGVTALLNIATRESEWD
                     EVLAAAEANDDVWASVGIHPHDADHHPDIDTAKLVERAAHPRVIGIGETGLDYYYDKS
                     DRARQQDSFRRHIHASQQTGLPIIVHTRDAEADTLALLGEEMDRAVFPGVIHCFTASD
                     DFARKALDLGLYISISGIVTFKNAADLQATAKWLPQDRLLIETDSPFLAPVPHRGKPG
                     EPAFVADTLAFLADLRGEDEDELAAVTSANFYALFNKAAP"
     gene            3771..4535
                     /locus_tag="VN034_00330"
     CDS             3771..4535
                     /locus_tag="VN034_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV59071.1"
                     /translation="MKLRILGCGTSSGVPRIGNDWGQCDPDNPRNLRSRASIMVSLGG
                     FRVLVDTSPDMRLQLLDAGVGEIDAVIWTHEHADHTHGLDDLRQVMHLRRSAVPVYAR
                     DHVLDILKWRFTYAFAGNAGYPASVDPIDLHDHQSIGPIEVSAIEMPHGPIKASGLIF
                     SDGAHRIAYATDFSKFTDEMVDFFQGVDLFVIDALRRYPHPTHPHLQMTLEGLAKVGN
                     PRAIITHMDNTMDYEDLAAELPAGVEPGYDGLEVQL"
     gene            4532..4708
                     /locus_tag="VN034_00335"
     CDS             4532..4708
                     /locus_tag="VN034_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59072.1"
                     /translation="MSGDTVVQTLWFAGAFALVLSSLLARRLPMADWLKMALAWVAIF
                     GLVFLVIRTWQTVT"
BASE COUNT          840 a         1525 c         1508 g          853 t
ORIGIN      
        1 cgcgcgcacc atccgcccgg cgttggcgcg cggcgcgtgg gtgctgtgcg accgcttcgt
       61 cgattcgagc cgcgcctatc agggcggcgg cggcgggatc accgatgccg atctcctcgc
      121 gttgcaccgc ttcggttcgg aaggactctt acccgaccgc actttcctgc tcaccgtcag
      181 ccccgacgag gcggcgcggc gcctcaccga gcgcgggggc agcgaccgca tggggaacaa
      241 acccttcgac tatcaggcgc gcctcgccgc gcgcttcgtc gaaatggccg cggccgaaac
      301 cggacgctgg cggatcgtcg acgccgaccg gcgcgccgaa gaggtgacag cggcaatcct
      361 cgcagacctt gcggaatggc tgccatgatc ggccatcagg aggccgagaa ggcatttctc
      421 gaagcgtggc agggcggccg cgtccatcat gcctggctgc tggcggggcc gcaggggatg
      481 gggaaggggg cttttgcgga gcgggtcgct cgctttctcg tcacgcacgg acgcggcggc
      541 gaaggtcaga cgacgacgct tgacgatcgc ggcgacgatg ccgccgcacg cctggtcgat
      601 gcgggcaacc accccgaagt cctgcgcctc gcccgccagc cgaaggacaa ggccaaggaa
      661 ctcgcgcgca acatcacgat cgagcaggtc cggcagatga tccgccgcct gcatctctcc
      721 ctgtcactcg gcgagtggcg cgtgatcatc gtcgatgcgg tcgacgacct cgaaaccgac
      781 ggggcgaacg cgctgctcaa aacgcttgag gagccgccgg caaaaacgct tttcctgctc
      841 gtcagccact cgcccggacg cctgttgccg acgatccgct cacggtgcag aaccctgcgt
      901 tttcaacccg ttgctcgtga catcatgacg acatggttgc acgacctgcg cccgatgatc
      961 gagatggagg aggtgcgcgc gatcgtcgcc gcatcgggcg gggttccggg caaggcgctt
     1021 gcgctcatcg atagcgatgt cgcggccatg gagaagaaat tgctcgcgat cgcggcgaac
     1081 ggcgatccgg agaataagct gcgcgaagcc ctcgcccgcg aggtcggcgg caccagtaat
     1141 cgggcccgtc tcgaattggt gatcgatatc gtgcccggcc tgctctcgcg catcgcgcgc
     1201 gaacgccctg tctcagagat cgcgccgatc ctcgcgcagt gggaccgcgt ccagcgcacc
     1261 gtccgcgatg cgatccgcgg atcctatgac ggcgcgatgg tcggctttga aatcggcaac
     1321 tgcctggccg aattggcgcc gcggcacgat cgggcggcac gctgacgctt tccctcccgc
     1381 gcggcagcgg ctaaggcgcg cgcatgtccg aaaataacga acctttctac atcaccaccg
     1441 cgatcagcta ccccaatggt cgtccgcata tcggccacgc ctatgaagcg atcgccaccg
     1501 atgtcatggc gcgtttccag cgcgcgcgcg gccgcgacgt tcggctcgtc accggcaccg
     1561 acgaacatgg gctgaagatg ttccagacgg cgcgcgatca gggccgcgcg acgatcgatc
     1621 ttgccgacga aatgtcggga tatttccgtg agatgtacgc caagctgaat atcagctatg
     1681 ataatttcat gcgcacgtcg gacgccgcgc atcatgccgc ctcgcaagcg atctggaaag
     1741 cgatggaggc caagggcgac ctctatctcg accgctatga aggctggtat tcggttcgcg
     1801 acgaggcttt ctacgacgaa agcgagctga gcgacgggga agggggcgtg cgcctttcac
     1861 cgcagggaac gccggtcgaa tggaccgtcg aggaaagctg gttctttcgc ctgtcgagct
     1921 atcaggaccg gctgctcgcg ctctataacg aacagcccga tttcattcgt cccgaaagcc
     1981 gccgcaacga ggtgctgcgc ttcgtcgagg gcgggctgaa ggatctcagc gtctcgcgca
     2041 ccagcttcga ctggggcgtg ccggtgccgg gatcgccggg acatgtgatg tatgtctggg
     2101 tcgacgcgct gaccacctat ctctcggcgc tcggttatcc cgatcccgac agcgacttcg
     2161 ggacattctg gccggcgaac attcatatga tcggcaagga tatcgttcgt tttcataccg
     2221 tttactggcc ggccttcctg atgagcgcga atttgccgct gccccagcag gtgttcggac
     2281 acggctttct gctcaatcgc ggcgagaaga tgtcgaaatc gctgggcaat gtcgtcgacc
     2341 cgatggcgct cgccgaccgc ttcggggtcg atgcgctgcg ttattatctg ctccgcgaag
     2401 tcagcttcgg ccaggacggt agctattcgg ccgaagcgat cgtgcgcacc gcgaacgccg
     2461 atctcgccaa cagcttcggc aatctcgctc agcgcagctt gtcaatgatt ttcaagaatt
     2521 tggacggcag actttcgggc gactatgcgc cagcccagga cgatatcgac ctgctggccg
     2581 acgtcgccaa catggcggcg gtccgcctgc cgcacgaatt cgaccagctc gccttttcgg
     2641 tgggcatcga ggattggctc cgcgccgtct tcgcctgcaa ccaatatgtc gatacgcagg
     2701 cgccgtgggc gctgcgcaag agcgaccccg agcgcatgcg cgcggtgctg atgacgctgt
     2761 ttcaggcggt ccgtacgctc gcgatcgcga tccgaccggt ggtgcccgcg gccgccgaca
     2821 aactgctcga ccagatgggc atcgccgagg atgcgcgcga tttcgcggca ctttctgaca
     2881 cggactggtt cacgaagctc gttgcaagcg gcttcgcggt cagccagcct gtcccgatct
     2941 tcccgcgcct cgaattgccc gagggagaag gggaggcgta atgctcgtcg attcgcactg
     3001 ccacctcaat tacaaggggc ttgccgagca gcagggcgaa gtgctggcgc gcgcgcgggc
     3061 aagaggcgtt accgcgctgc tcaacatcgc gacgcgtgaa agcgaatggg acgaggtgct
     3121 cgcggcggcc gaggcgaacg acgatgtctg ggccagcgtc ggtatccatc cgcacgatgc
     3181 cgaccatcat cccgacatcg acacggccaa gctggtcgaa cgcgccgcgc acccgcgcgt
     3241 gatcggaatc ggcgagaccg ggctcgacta ttattacgac aagagcgacc gggcgcggca
     3301 gcaggatagt ttccgccgcc atatccatgc ctcgcagcag accggcctgc cgatcatcgt
     3361 ccatacgcgc gatgccgagg cggatacgct ggcattgctc ggcgaggaga tggaccgcgc
     3421 ggtctttccc ggcgtgatcc actgcttcac cgcgagcgac gatttcgcgc gcaaggcgct
     3481 cgatctcggg ctttacatct cgatttcggg aattgtgacc ttcaagaatg ccgccgatct
     3541 tcaggcgacc gccaaatggc tgccccagga ccggctgctg atcgaaaccg actcgccctt
     3601 cctcgccccc gtcccgcacc ggggcaagcc cggcgagccc gccttcgtcg ccgacacgct
     3661 ggcgttcctc gccgacctgc gtggtgaaga cgaggatgag ctcgcggcgg tcacgagcgc
     3721 caatttctat gcgcttttca acaaggcggc gccatgacgt cacgcgcgca atgaagctga
     3781 gaatcctggg ttgcgggacg tcatcgggcg tgccgcggat cggcaacgac tggggccagt
     3841 gcgatccaga caatccccgc aacctgcgca gccgcgcttc gatcatggta tcgctcggcg
     3901 gctttcgggt ccttgtcgac accagccccg atatgcggct gcagctgctc gacgcggggg
     3961 tcggcgagat cgacgccgtc atctggacgc acgagcatgc cgaccacacc cacggtctcg
     4021 acgatctgcg ccaggtcatg cacctgcgcc gctcggcggt tccggtctac gcccgcgatc
     4081 atgtcctcga catattgaag tggcgcttca cttacgcctt cgccggcaat gcaggctatc
     4141 cggcgtcggt cgatccgatc gatctccacg atcatcaatc gatcggcccg atcgaggttt
     4201 cggcgatcga gatgccgcac ggaccgatca aggcgagcgg gctgatcttc agcgacggtg
     4261 cgcacagaat cgcatatgcc actgattttt ctaaatttac cgacgaaatg gtcgattttt
     4321 ttcagggcgt cgacctgttc gtcatcgacg cgctgcgccg ctacccccat ccgacgcatc
     4381 cgcaccttca gatgacgctg gaggggctgg ccaaggtcgg caacccgcgc gcgatcatca
     4441 cgcatatgga taatacgatg gattatgaag accttgcggc tgaattgccc gcaggggtcg
     4501 aaccgggcta tgacgggttg gaggtccagt tatgagtggt gataccgtcg tccagacgct
     4561 gtggttcgcc ggcgcgttcg cgctggtgct cagctcgtta ctcgcgcgcc gcttgccgat
     4621 ggccgactgg ctcaagatgg cgctcgcatg ggtcgcgatc ttcgggctgg tgttcctcgt
     4681 gatccggaca tggcagacgg tgacataaag agggcgggcc gccgtg
//
LOCUS       DATMSU010000013         4402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_9793, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000013 DATMSU010000000
VERSION     DATMSU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9793"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1388
                     /locus_tag="VN034_00340"
     CDS             189..1388
                     /locus_tag="VN034_00340"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV59073.1"
                     /translation="MPAFRFKSSTAEEAGFSASALGELDDYFDTMVANGELPGQVMLL
                     ARGDRLVHSHVTGFADCRDRRPLDADAIFTLFSMSKPLAAVAMLLLHEDGQWQLDDPV
                     SDYLPEFADIASLPGSRATRAPTIRELFTHSAGFSLGKTVEEMQAHIAKLSWKDARSL
                     TQQIGRYASLPLGYEPGTAWEYSIATDLLAEIVERLSGERYDLFLKKRVFDPLGMTDT
                     GFVLDYDQSRRLVCGHVLDPETGRLRVATADERMESIFPMGGTSFKSTAGDFALFARM
                     LLERGSLGEVQILKPESVDLMLSNHLPDALLESRFPILHYVIGDGNGHGLNGMVCLDP
                     ERAGRPVGKGTYEWGGAFGTWFWIDPEHDILCVGMTHRQRIATDLRPPEVVAQELVYR
                     AFRETRP"
     gene            1417..2286
                     /locus_tag="VN034_00345"
     CDS             1417..2286
                     /locus_tag="VN034_00345"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWV59074.1"
                     /translation="MSFSLQTPVDQFEMPHPSGTGTFKISVARVAQVPGDAKVPVLYV
                     LDGDIGFALAAEIARMRGLMGMQPTAMVVGIGYGADFLEFAKLRTADLTPPLSEAGRS
                     ALGNMTSFIGEQDGGAEAFLDFLTETLAPEIGRRYPEASADERLLFGHSLGGLFAVYA
                     LLTRPGAFSAFIANSPSLWWDGFAILDRLPAFAEKLAALASPPRVLIGVGGQEQDVPT
                     KVPPGLQMSLAEVQATVAASRMVDAVPELAAALADAGLRDVEYVIYDKEDHGSVVPAA
                     MMRGLRFAVPDRT"
     gene            complement(2361..2435)
                     /locus_tag="VN034_00350"
     tRNA            complement(2361..2435)
                     /locus_tag="VN034_00350"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2401..2403),aa:Gln,seq:ttg)
     gene            complement(2494..3579)
                     /locus_tag="VN034_00355"
     CDS             complement(2494..3579)
                     /locus_tag="VN034_00355"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HWV59075.1"
                     /translation="MSAPAFTHLPHPNPAADDVRAAAIADPLFGRVFTDHMVSIRYTE
                     GKGWHDAQVMPRGPLTLDPATAVLHYAQEIFEGLKAYRLDDGSMALFRVEANAARFNA
                     SARRMAMAELPEEMFIAAVKEAVAADAHWFPPVDGGALYLRPFMFASEVFLGVKPASE
                     YQFLVITSPVGNYFKSGAPAISLWVSEDYTRAAPGGTGAAKCGGNYASSLVAQREAIA
                     KGHDQVVFLDAAEHRWIEELGGMNMFFVFDDGSIVTPPLTGTILPGITRDAIITLATD
                     AGLTVREEPYAIDQWQSDAESGKLTESFACGTAAVVTPVGKVTRGEASFTIGAGGPGQ
                     VTEMLKSKLVDIQRGRAADPHGWVTRL"
     gene            3658..4170
                     /locus_tag="VN034_00360"
     CDS             3658..4170
                     /locus_tag="VN034_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWV59076.1"
                     /translation="MPDENFLSQVPAASALFLREEEVRRGIEFLFFAHASLWRAIDAR
                     LAEKELGRAHYRALYFIARQPGLTISDLLALLGITKQSLGRVVKELEAREYLTTRPGN
                     RDRRAKELRLTDAGRVAEREIFAALRDTMSRAYTHAGQQAVTGFWQVSEALVPPRERR
                     RIAELGKDAG"
BASE COUNT          767 a         1441 c         1411 g          783 t
ORIGIN      
        1 gctaatgatc tccagaatac ccaatcaact tattgtaatt aaatatattt ttgaacgata
       61 aaacaaatgg cttgaccgac ggagttttcc atactttcct attgggaaag aaaagttgaa
      121 tacagtgaag gatgatcgcc gattcgactc ggcgggagcc tgcgtgccgc agacatggga
      181 gaaaccggat gccagcgttc cgcttcaaat cctcgaccgc cgaagaggca ggcttttcgg
      241 ccagcgcgct cggcgagctc gacgattatt tcgacacgat ggtggcgaat ggcgaactgc
      301 ccgggcaggt gatgctcctc gcccgcggcg acaggctggt ccattcccat gtcaccggct
      361 tcgccgactg ccgcgaccgc cggccgctcg acgccgacgc gatcttcacc cttttctcga
      421 tgagcaagcc gctggcagcg gtcgcgatgc tgctactcca cgaagacggg caatggcagc
      481 tcgacgatcc ggtctcggac tatcttcccg agttcgcgga catcgcgtcg ctccccggta
      541 gccgggcgac gcgggcgccg accattcgcg aactcttcac gcacagcgca ggcttcagcc
      601 tcggcaagac cgtcgaggag atgcaggccc atatcgcgaa gctttcgtgg aaagacgcgc
      661 gctcgctgac gcagcagatc ggtcgctatg cgtcgctgcc gctgggctac gaacccggta
      721 ccgcatggga atatagcatc gcgaccgacc tcctggccga gatcgtcgag cggctgagcg
      781 gcgaacgtta cgatctgttc ctcaagaagc gcgtcttcga tccgctcggc atgaccgata
      841 ccggtttcgt gctcgactat gaccagtcgc gccggctggt ctgcggtcat gtcctcgatc
      901 ccgaaacggg tcggctgcgc gtcgccacgg ccgacgagcg gatggaatcg atctttccga
      961 tgggcggcac gtcgttcaag tcgaccgccg gggatttcgc gctcttcgcg cgcatgctgc
     1021 tcgagcgcgg cagccttggc gaggttcaga ttctcaagcc cgagagcgtc gacctgatgc
     1081 tgtcgaacca tcttcccgac gcgctgctcg aaagccgctt tcccatcctc cactatgtca
     1141 tcggcgacgg caacgggcac ggcctcaacg gcatggtctg cctcgacccc gaacgcgccg
     1201 ggcgcccggt cggcaagggc acctatgaat ggggcggcgc cttcgggacc tggttctgga
     1261 tcgaccccga acacgacata ttgtgcgtgg gcatgacgca tcgccagcgc atcgccaccg
     1321 acctgcgccc gcccgaggtg gtcgcgcagg aacttgtcta tcgcgcgttc cgcgaaaccc
     1381 gcccctgatt tcatccacga caagaaagcc gatcctatga gcttttccct gcaaacgccc
     1441 gtcgaccagt tcgaaatgcc gcatccgagc ggcaccggca ccttcaagat cagcgtcgcg
     1501 cgcgtggcgc aggtgccggg ggacgcaaaa gtccctgtcc tctatgtgct cgacggcgac
     1561 atcggcttcg cactggccgc cgaaatcgca cggatgcgag ggctgatggg gatgcaaccg
     1621 accgcgatgg tagtaggcat cggctatggc gccgatttcc tcgaatttgc caaactacgc
     1681 accgccgacc tgaccccgcc gctcagcgaa gcagggcggt cggcgctagg caatatgacg
     1741 agcttcatcg gcgagcagga cggcggcgcc gaggcctttc tcgacttcct gaccgagaca
     1801 ctggctcccg agatcgggcg gcgttatccc gaagcgtcgg cggacgaacg tctcttgttc
     1861 ggccattcgc tcggcggtct gttcgccgtc tacgctctgc tgacgcggcc cggtgccttc
     1921 agcgccttca tcgccaacag cccctcgcta tggtgggacg gcttcgcgat cctcgatcgc
     1981 ctccccgctt ttgccgaaaa gctcgcggcg ctcgcaagcc caccgcgtgt cctaatcggc
     2041 gtcggtggtc aggaacagga tgtgccgacg aaggtgccgc cgggcttgca gatgtcgctc
     2101 gccgaagttc aggcgaccgt cgccgcatcg cgcatggtgg acgcggttcc cgaactcgcc
     2161 gccgcgctcg ccgatgcggg cctgcgcgat gtcgaatatg tgatctatga caaggaagac
     2221 catggcagcg tcgtgccggc cgcgatgatg cgcggcctgc gctttgcggt cccggaccga
     2281 acgtagaggc ggcagcatgc gtcaaccgcc gttcccggtc acgacaattc gaaggtgtgg
     2341 ggcgacgaga attgaccggc tggctggggc ggcaggattc gaacctgcga atggcggcat
     2401 caaaagccgc tgccttaccg cttggcgacg ccccaacggc cggtgcgacg cgcgctctat
     2461 agcgcgtcgc cgcttaatgc aaggcgggtc ggatcagagc cgcgtgaccc agccgtgcgg
     2521 gtcggccgcg cggccgcgct ggatgtcgac cagcttgctc ttcagcattt cggtgacttg
     2581 tcccggaccg ccggcgccga tcgtgaagct cgcttcgccg cgcgtcacct tgccgaccgg
     2641 ggtcaccacc gcggcggtgc cgcaggcgaa gctttcggtc agcttgccgc tttccgcatc
     2701 gctctgccac tggtcgatcg catagggttc ctcgcgcacc gtcaggccgg cgtccgtcgc
     2761 cagcgtgatg atcgcgtcgc gcgtgatgcc gggcaggatc gtgccggtca gcggcggcgt
     2821 gacgatgctg ccgtcgtcga agacgaagaa catgttcatg ccccccagtt cttcgatcca
     2881 gcgatgctcg gcggcgtcga ggaagacgac ctggtcgtgc cccttggcga tcgcttcgcg
     2941 ctgcgcgaca aggctggagg catagttgcc gccgcacttg gcggcgccgg tgccgcccgg
     3001 cgcggcgcgg gtataatcct ccgacaccca gagcgagatc gccggggcgc ccgatttgaa
     3061 ataattgccg accggcgagg tgatgacgag gaactgatat tcggacgcag gcttcacgcc
     3121 gaggaagacc tcgctcgcga acatgaaggg gcgcagatag agcgcgccgc cgtcgaccgg
     3181 cgggaaccag tgcgcatcgg cagcgacggc ttctttcacc gccgcgatga acatctcctc
     3241 ggggagttcg gccatcgcca tccggcgcgc gctcgcgttg aagcgcgcgg cattggcctc
     3301 gacgcggaac agcgccatcg agccgtcgtc gagcctatag gccttcagcc cctcgaagat
     3361 ttcctgcgca taatggagca ccgcggtcgc gggatcgagc gtcagcggcc cgcgcggcat
     3421 cacctgcgca tcgtgccagc ccttcccctc ggtgtagcgg atcgacacca tatggtcggt
     3481 gaaaacgcgt ccgaaaagcg ggtccgcaat cgccgccgcc cgtacatcgt ccgccgccgg
     3541 attggggtgc gggagatggg tgaaggcggg agcggacatg cggaaacacc tttgctttgc
     3601 tggtttcggg agcgcgcctc ttgccaaaga gcggcacggc gcgcaacggt agcgactatg
     3661 ccggatgaaa attttctttc acaagttccc gccgcctctg cccttttctt gcgcgaagag
     3721 gaagtgcgcc gcggcatcga gttcctcttt ttcgcgcatg cttcgctctg gcgcgcgatc
     3781 gatgcacggc ttgccgagaa agaactcggg cgcgcgcatt accgcgcgct ctatttcatc
     3841 gcgcggcagc cgggattgac gatttcggat ctgctcgcgc tgctcggcat caccaaacag
     3901 tcgttggggc gcgtggtgaa ggagctcgaa gcgcgcgaat atctgacgac gcggccggga
     3961 aaccgcgacc ggcgcgccaa ggagctgcgg ctgaccgacg ccggccgcgt ggccgaacgc
     4021 gagatattcg cggcgctccg cgatacgatg agccgcgcct atacccatgc gggacagcag
     4081 gcggtgaccg gcttctggca ggtcagcgag gcgctcgttc cgccgcgcga acgccgccgg
     4141 atcgcggagc tcgggaagga cgcgggttaa cggcccgcca ttccagaatc cgttcgcatc
     4201 gaacgaagtc gagatgcccg tcggccaagc actacattga tgggtgtctc gacttcgctc
     4261 gacacggacg gaatggatag cgcttacccc tcgccgcgcg cgatgtccgc gagctcgcgc
     4321 ccgcggtcgc gcgcggcgcg cagcacgttg gtgagcaatg cggcgagctg gccgtcgctg
     4381 tcgagcacat cgagccccgc ct
//
LOCUS       DATMSU010000014         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_57169, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000014 DATMSU010000000
VERSION     DATMSU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57169"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1499)
                     /gene="nuoN"
                     /locus_tag="VN034_00365"
     CDS             complement(66..1499)
                     /gene="nuoN"
                     /locus_tag="VN034_00365"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HWV59077.1"
                     /translation="MTADLALIWPELILTIGGLITLMLGTFMGDRQVRIYQLSSLLTL
                     SAAAAAVVALIGVETTVFSGTLSVDGFGGFAKLLIYAASFICIMIAPRFFCGSMRAEY
                     PTLILFAALGMGIMASSRDLMTLYVGLELNSLAAYVLASFMRTDERSSEAGLKYFVLG
                     ALASGMLLYGISLLYGFSGTTDFAGIAKAMGGELNIGLIFGIVFVLAGLGFKISAVPF
                     HMWTPDVYEGAATPVTTFFATAPKVAAMALMTRVVIDAMGPAVGAWQQIIIFLALASI
                     ILGAVGAIGQKNIKRLLAYSSINNVGFMLIGLAAGTQQGVEGVLTYLLVYMLTTLGAF
                     LVVLQLRDADGNHVESIPALAGLSQRRPGLAAAMAVFLFSLAGIPPLFGFWPKYLVFE
                     AAVNANLVPLAVAGIVASVIGAFYYIAIIKTMYFDDKSDTEFPKGGGAIVEDAVITAS
                     ALWLSVIGYLFIPALAVLSAGAAAVLF"
     gene            complement(1496..3049)
                     /locus_tag="VN034_00370"
     CDS             complement(1496..3049)
                     /locus_tag="VN034_00370"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWV59078.1"
                     /translation="MSGFPILSLMLAVPAIAAILCLFVGDKTARFVALGATLATFVLG
                     CILWANFDIGGAQWQFTERADLFGRFQYALGIDGMALMLIMLSVFLMPLCILASWDAI
                     KQRVGLYMSMFLIMETIMIGVFMAQDLLLFYIFFEAGLIPMYFIIGIWGGANRKYAAF
                     KFFLYTLLGSVLMLIAMIAMIAEAGTTDIPTLLNYDFPVQMQTWLWLAFFASLAVKMP
                     MWPVHTWLPDAHVQAPTAGSMILAGVLLKLGAYGFIRFMMPMFPDASAQLMWIVFGLS
                     MIAVVYTSLVALAQSDMKKLIAYSSVAHMAIVTAGLFAFNQQGIEGALIIMLSHGVVS
                     AALFFCVGVIYDRLHTREIDRYGGLAVNMPAYALFFMLFTMASIGLPGTSGFVGEFLS
                     LLGIYEASSVVAFVCTTGIILGAAYMLYLYRRVVFGELTKDDVKAMPDLNAREWAIMA
                     PLAAVALWMGVYPESFLAPMRGDVTTVVARLAPAKPAGDAHLAAGKPKPAAAHGEAAH
                     GSSHAGGVQ"
BASE COUNT          695 a          955 c         1034 g          468 t
ORIGIN      
        1 tgccagccgc gccagcaggt cgtcgttggt cgagcctgtc tgctgggtcc gctcgatcgg
       61 cgggatcaga agagcaccgc cgccgcgccg gccgaaagaa ccgccagcgc cggaatgaag
      121 agatagccga tgaccgagag ccatagggcg ctcgcggtga tcacggcatc ctcgacgatc
      181 gcgccgccgc ccttcggaaa ttcggtgtcc gacttgtcgt cgaaatacat cgtcttgatg
      241 atcgcgatat aatagaaagc gccgatcacc gaagcgacga tgcccgcgac cgccagcggc
      301 acgaggttcg cgttcaccgc ggcctcgaac accagatatt tcggccagaa accgaacagc
      361 ggcgggatgc cggcgaggct gaacaggaac accgccatcg ccgcggcgag ccccggacgg
      421 cgctgcgaca ggccggcgag cgccgggatg ctttcgacat ggttgccgtc ggcatcgcgc
      481 agctggagca cgacgaggaa ggcgccgagc gtcgtcagca tatagacgag cagataggtg
      541 agaacgcctt cgaccccctg ctgcgttccg gcggcgagac cgatgagcat gaagccgaca
      601 ttgttgatcg acgaataggc gagcaggcgc ttgatattct tctgcccgat cgcgccgacc
      661 gcgccgagga tgatcgaggc gagcgcgagg aagatgatga tttgctgcca cgcgccgacc
      721 gcagggccca tcgcgtcgat cacgacgcgt gtcatcagcg ccatcgccgc gaccttgggc
      781 gcggtggcga agaaggtcgt caccggggtc gctgcgcctt catagacgtc gggcgtccac
      841 atgtggaacg gcacggcgct gatcttgaag ccgaggcccg cgagcacgaa gacgatgccg
      901 aagatcagcc cgatgttgag ctcgccgccc atcgccttcg cgatgcccgc gaaatcggtg
      961 gtgcctgaaa agccgtagag cagcgaaatg ccatagagca gcatgcccga ggcgagcgcg
     1021 ccgaggacga aatatttgag gccggcttcg ctcgaccgtt cgtcggtgcg catgaagctc
     1081 gcaagcacat aggcggcaag gctgttgagc tcgagcccga cataaagcgt catcaggtcg
     1141 cgcgacgagg ccatgatccc catgccgagc gcggcgaaca ggatcagcgt cggatattcg
     1201 gcgcgcatcg aaccgcagaa gaagcgcggc gcgatcatga tgcagatgaa gctcgccgca
     1261 tagatcagca gcttggcaaa gccgccgaag ccgtcgaccg aaagcgtgcc cgaaaacacg
     1321 gtcgtctcga ccccgatcag cgcgacgacg gcggccgcgg cggcagagag cgtgagcagc
     1381 gagctcagct ggtaaatgcg gacctgccgg tcacccatga aggtgccgag catcagcgtg
     1441 atcagcccgc cgatcgtcag gatcagttcg ggccagatga gcgcgagatc ggcggtcatt
     1501 ggacgcctcc cgcatggctc gatccgtggg cggcttctcc atgcgctgcg gcgggcttgg
     1561 gcttgccggc cgcaagatgc gcgtcaccgg cgggcttcgc cggcgcgagg cgcgcgacga
     1621 ccgtcgttac gtcgccgcgc atcggcgcga ggaagctttc gggatagacg cccatccaca
     1681 gcgccacggc ggcgagcggg gccatgatcg cccattcgcg agcgttgaga tcgggcatcg
     1741 ccttcacatc gtccttggtg agttcgccga acacgacgcg gcggtagaga tagagcatgt
     1801 atgcggcacc gaggatgatg ccggtggtgc acacgaaggc gacgacgctc gacgcttcat
     1861 aaatgccgag caggctcagg aattcgccga cgaagccgct ggtgcccggc aggccgatcg
     1921 acgccatggt gaacagcatg aagaaaagcg cataggccgg catgttcacc gcaagcccgc
     1981 cgtaacggtc gatctcgcgc gtgtggagcc ggtcatagat cacgccgacg cagaagaaga
     2041 gcgcggccga gacgacgccg tggctgagca tgatgatcag cgcgccttcg atgccctgct
     2101 ggttgaaggc gaagagaccc gcggtgacga tcgccatatg cgcgaccgac gaataagcga
     2161 tcagcttctt catgtcgctc tgcgccagtg cgacgaggct ggtgtagacc accgcgatca
     2221 tcgacaggcc aaaaacgatc cacatcaatt gagcggacgc atcggggaac atcggcatca
     2281 tgaagcggat gaagccatag gcgccgagct tcaagagcac acccgcgagg atcatcgagc
     2341 ctgccgtggg cgcctggacg tgcgcgtcgg gaagccaggt gtggacgggc cacatcggca
     2401 tcttgaccgc gaggctggcg aagaaggcga gccacagcca cgtctgcatc tgaaccggga
     2461 aatcatagtt cagcagcgtc ggaatatcgg tcgtgcctgc ctctgcaatc atcgcgatca
     2521 tcgcgatcag catcagcacc gatccgagca gcgtgtagag gaagaatttg aacgccgcat
     2581 atttgcggtt cgcgccgccc cagatgccga tgatgaaata catcggaatc agcccggctt
     2641 cgaagaagat gtaaaagagc agcaggtcct gcgccatgaa gacgccgatc atgatggttt
     2701 ccatgatcag gaacatcgac atgtagaggc cgacgcgttg tttgatcgcg tcccagctgg
     2761 cgaggatgca gagcggcatc aggaagacgc tgagcatgat cagcatcagc gccatgccgt
     2821 cgatgccgag tgcatattgg aagcgcccga acaggtcggc gcgctcggtg aactgccact
     2881 gcgcgccgcc gatatcgaag ttcgcccaca ggatgcagcc gagaacgaaa gtcgcgagcg
     2941 tcgcgccgag cgcaacgaag cgcgctgtct tgtcgccgac gaacaggcaa aggatggcgg
     3001 cgatcgcggg gaccgccagc atcagcgaaa ggatcggaaa gccgctcaca ggaaattcac
     3061 catggcccag ctggcgaggc cgacaagacc gagcagcatc acaaacgcat atccataaac
     3121 ataacccgat tgcagccgga ccgcgagacg gg
//
LOCUS       DATMSU010000015         4893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_142537,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000015 DATMSU010000000
VERSION     DATMSU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142537"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VN034_00375"
     CDS             <1..639
                     /locus_tag="VN034_00375"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWV59079.1"
                     /translation="GWGAAPRPFGPAVLADKPEQRFNDGRVDAAGRLWVGSLTSDKAN
                     PTATLYRLDPDGSLTEIFGRLTTSNGAAFSPDGRTFYHADTPTHAIRAYDVDPAAGTL
                     SNGRIFHQFEFGNGRPDGAAVDAEGCYWSALWDGWRVVRLSPAGELLQTVDLPVQRPT
                     MIAFGGPDLQTAFVTSAGKNLSDEERAKQPHAGGVFSFRVDVPGLIQRDFGA"
     gene            726..2924
                     /locus_tag="VN034_00380"
     CDS             726..2924
                     /locus_tag="VN034_00380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWV59080.1"
                     /translation="MSRTAQPFARHLSMMLASTAMIVGYSQMTTATAAEPTAATKLVP
                     ATPVGQNPMLAPWTGPFEGVPPWDKMDPELFPDAFVKGMAETKAQVQAVIDNPAAPTF
                     ENTHVPMMLAGDTMDRLFALWGVQTSNKSNDRVEDIDAEWSPKLTTFYTELFLEPKLF
                     ARYKAVYDKRNNSGLNAQQIRIVERSYDEMVRDGANLSPADKTKLVAMNSKLEGLFSA
                     FSSKLLGDEKLYTFVTDKAELAGLEPGFVASLAAAAEANGKPGQWAIKNTRSSAQPVM
                     QGATNRALRERVYKAFISRGDNGDANDTNATIVEILKLRQQRAELLGFPTHAHYRMAD
                     TMAKTPEAAMGLMMKVWPAAVARVKEEVADMQAIADAEAKAGKGPKITIEPWDYRFYS
                     EKVRKAKYDLDESEVKPYLQLDKLRDAMFWSAGQLYDLGFRENTGTIPVFDPKVRTFE
                     VYNLKTNENVGVFYLDNFARDGKRSGAWMTTYRSQQTLGGERNVLASNNNNFTEAAKG
                     EPTLISLDDATTLFHEFGHGIHYLLQHIYYPALAGVPRDFVEYPSQVNENWLMTPEVL
                     SKYATHYKTGEPMPQALVDKILASDKFNQGFSTVEYLSSAIVDMKLHDRKTPPTDVDK
                     FERETLAEIGMPKELVMRHRLPQFGHLFSSDAYSAGYYSYLWSETMDADTWAAFTEAG
                     GPWDRGVADKFRTILLMTGNETDRAEAYRAFRGRDPDVKALLKKRGFPTE"
     gene            complement(2976..3746)
                     /locus_tag="VN034_00385"
     CDS             complement(2976..3746)
                     /locus_tag="VN034_00385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV59081.1"
                     /translation="MLQGKKLVQSMFARSGYRIVRSESGMRYRPTAADARHSASLLDE
                     ILPVVQHNALTGEWPTPAFLEDYFDASRLAMARLLLDQCDAEDVEIAGKRVLDIGCHA
                     GSLLRLMHARYPEASLFGCDISDVKLAMAKRACPDAELFFSALSDLPRSSRYDVVFLM
                     EVLEHTVDPEAVVRRLLDIVSAAGTLILTVPDGRKDQFPAKEYHAEFDSYAGHINFWS
                     PESWRYFLMRTAPEWKLRTGTLPTGHLFAALSMKNKVN"
     gene            4010..>4893
                     /locus_tag="VN034_00390"
     CDS             4010..>4893
                     /locus_tag="VN034_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWV59082.1"
                     /translation="MVETGTPALNRSRETRAATQSGYLMLFIWLLLLGVAAWAGITNA
                     NAEYMVAWKWIIVVASAVVGLLILCGFYLINPNEAAAIQLFGAYKGTDREEGLRWVLP
                     WLTRKKIAVRANNVISDKIKVNDLRGNPIEMAAQVVWRVTDTAQALFDVDDYKEFVMA
                     QIEAAVRSIGSRYPYDDIEHQEVTLRGNHDEVGVELRKALIERLSVAGITVDECGLTH
                     LAYAQEIAGAMLRRQQAEAVIAARKKLVEGAVTMVEMALTQLSEKGVVDLDDERKAVM
                     VSNLMVVLCGERDTQPVV"
BASE COUNT          973 a         1564 c         1521 g          835 t
ORIGIN      
        1 gggtggggtg ccgcaccgcg tccgttcggg ccagcggtgc tcgccgacaa acccgaacag
       61 cgcttcaacg atggccgtgt cgacgccgcg gggcggctgt gggtcggcag cctgacgagc
      121 gacaaggcga accccacggc cacgctctac cggctcgatc ccgatggctc gctgaccgaa
      181 atcttcggac ggctgacgac gagcaacggc gcagccttca gccccgacgg ccgcaccttc
      241 taccacgccg acacgccgac gcatgcgatc cgcgcctatg atgtcgaccc ggccgcgggc
      301 acgctgagca acggccgcat cttccaccag ttcgagttcg gaaacggccg ccccgacggc
      361 gccgcggtcg atgccgaggg ctgttactgg tcggcgctgt gggacggctg gcgcgtcgtg
      421 cgcctctcgc ccgcgggcga attgctccag accgtcgatc tgcccgtcca gcgtccgacg
      481 atgattgcgt tcggtgggcc cgacctccag accgccttcg tcaccagcgc gggcaagaac
      541 ctgtccgacg aagagcgcgc gaagcagccg cacgcgggcg gcgtgttcag cttccgcgtc
      601 gacgtgcctg gcctgatcca gcgggatttt ggcgcctagg cggcttgcac cccgctgtca
      661 tataaggtta aggcggcccc cgacatggtt tcgttcgaaa ccttatcctc cggggagatt
      721 tatcgatgtc gcgcaccgcg cagccttttg cccgtcattt gtcgatgatg cttgcatcca
      781 ccgcgatgat cgtgggttat agccagatga ccaccgcaac cgccgccgaa ccgaccgccg
      841 ccacgaagct ggtccccgcc acgcccgtgg gccagaaccc gatgctcgcg ccgtggaccg
      901 gcccgttcga aggggtgccg ccgtgggaca agatggatcc cgagctgttc cccgacgcct
      961 tcgtgaaggg catggccgaa accaaggcgc aggtgcaggc ggtgatcgac aatcccgcgg
     1021 cgccgacgtt cgagaacacg cacgtcccga tgatgctcgc gggcgacacg atggatcgcc
     1081 tcttcgcgct gtggggcgtg cagacgtcga acaagtcgaa cgaccgcgtc gaggacatcg
     1141 acgccgaatg gagccccaag ctcaccacct tctacaccga gctgttcctc gagccgaagc
     1201 tcttcgcacg ctacaaggcg gtgtacgata agcggaacaa cagcggcctc aacgcgcagc
     1261 agatccgcat cgtcgaacgc agctatgacg aaatggtccg cgacggcgcg aacctgtcgc
     1321 ccgccgacaa gacgaagctg gttgcgatga actcgaagct cgaaggcctg ttctcggcct
     1381 tctcgtcgaa gctgctcggc gatgaaaagc tctacacctt tgtcaccgac aaggccgagc
     1441 ttgcgggtct cgagcccggc ttcgtcgcct cgctcgccgc ggcggccgaa gccaatggca
     1501 agcccggcca gtgggcgatc aagaacaccc gctcgtcggc gcagccggtg atgcagggcg
     1561 cgaccaaccg tgcgcttcgc gagcgcgtct acaaggcgtt tatcagccgc ggcgacaatg
     1621 gcgacgcgaa cgacaccaat gcgacgatcg tcgagatatt gaagctgcgc cagcagcgcg
     1681 ccgaactgct cggcttcccg acccacgcgc attaccgcat ggccgacacg atggcgaaaa
     1741 cgcccgaagc cgcgatgggc ctgatgatga aggtctggcc cgccgcggtc gcgcgcgtga
     1801 aggaagaggt cgccgacatg caggcgatcg ccgacgccga agccaaggcc ggcaagggtc
     1861 cgaagatcac catcgaaccg tgggattatc gcttctattc ggaaaaggtc cgcaaggcga
     1921 aatacgacct cgatgaaagc gaggtgaagc cgtacctcca gctcgacaag ctgcgcgacg
     1981 cgatgttctg gtcggcgggc cagctctacg acctcggctt ccgcgagaac accggcacga
     2041 tcccggtctt cgatcccaag gtccgcacct tcgaggttta taatctcaag acgaacgaga
     2101 atgtcggggt cttctatctc gacaatttcg cgcgcgacgg gaagcgctcg ggcgcgtgga
     2161 tgacgaccta tcgcagccag cagacgctcg gcggcgaacg caacgtcctc gcgtcgaaca
     2221 acaataattt caccgaagcc gcgaagggcg agccgacgct gatcagcctc gacgatgcga
     2281 cgacgctgtt ccacgaattc ggccacggca tccattatct gctgcagcac atctattatc
     2341 ccgcgctcgc cggggtgccg cgcgacttcg tcgaatatcc gagccaggtg aacgagaatt
     2401 ggctgatgac ccccgaagtg ctgtcgaaat atgcgacgca ctacaagacg ggtgagccga
     2461 tgccgcaggc gctggtcgac aagatcctcg cctccgacaa gttcaaccag ggcttttcga
     2521 cggtcgagta tctgtcgagc gcgatcgtcg acatgaagct gcacgaccgc aagacgccgc
     2581 cgaccgacgt cgacaagttc gagcgcgaga cgctcgccga aatcggcatg ccgaaggaac
     2641 tggtgatgcg gcaccgcttg ccgcagttcg gccatctgtt cagctcggac gcctattcgg
     2701 cgggctatta cagctatctt tggtccgaaa cgatggacgc ggacacctgg gccgccttca
     2761 ccgaagccgg cggcccgtgg gaccgcggcg ttgccgacaa gttccgcacc atcctgctga
     2821 tgaccggcaa cgaaaccgac cgcgccgaag cctatcgcgc cttccgcggc cgcgatcccg
     2881 acgtgaaggc gctgttgaaa aagcgcggtt tcccaaccga ataagcgaac gataccgggg
     2941 aggcaaaggg ggcgatttcg ccccctttgt ttatcctaat tcaccttatt cttcatgctt
     3001 agcgctgcga acaaatgtcc ggtcggcagc gttcctgtac gaagtttcca ctcgggagcg
     3061 gtccgcataa ggaaatagcg ccagctctcc ggactccaga agttgatgtg gcccgcgtag
     3121 gaatcaaact ccgcatgata ttccttggcg gggaattgat ccttccgccc atcgggaacc
     3181 gtcaatatga gcgtcccggc agcggacaca atatcgagca ggcgccggac aacggcttcg
     3241 ggatcgaccg tgtgctcaag cacttccatc aggaacacca cgtcgtagcg cgatgaccgg
     3301 ggaaggtccg aaagcgcgct gaagaagagt tcggcgtccg ggcaggcgcg tttggccatc
     3361 gcgagcttca catcgctgat gtcgcatccg aagagcgagg cttccgggta tctggcgtgc
     3421 atgaggcgca gcaggctccc tgcgtggcac ccgatgtcca gaacgcgctt gcccgcgatt
     3481 tcgacgtctt cggcgtcgca ttgatcgagc agaaggcggg ccatcgcaag gcggctggca
     3541 tcgaaataat cctcaagaaa tgccggtgtc ggccattctc cggtcagcgc attgtgctgc
     3601 accacgggaa gtatctcatc gagcaagctg gcagagtggc gcgcgtcggc tgcggtcggc
     3661 cgataccgca tgccggattc tgatcttaca atcctgtagc cgctccgcgc gaacatggat
     3721 tgaaccagtt ttttgccctg gagcatctgt cctccttcgc gcacgagcgc gtcagttttg
     3781 aagtccgaac ttgagtatgc ttcgatgctg ccgcagccgg ccgccatggg caaccacacc
     3841 gattgccaca catcccggtc tgaacacgat cgaacccaac tccggccgct gtgccagctt
     3901 gccgcagcga aaccgacatg agcgtctgtt cgctcgaatc ccgattgacc tccacccgat
     3961 tcgatataaa tatgatatca tctttatatc gaatgattcg gagggcaaaa tggtcgaaac
     4021 agggactccc gcgctgaacc gcagccgcga gacacgcgcc gctacgcaga gcggctatct
     4081 gatgctgttc atctggctgc tgctgctggg cgtcgccgcc tgggcgggga tcaccaacgc
     4141 caatgctgaa tatatggtcg cgtggaaatg gatcatcgtt gtcgcctcgg cggtcgtggg
     4201 cctgctgatc ctgtgcggct tctacctcat caacccgaac gaggccgcgg cgatccagct
     4261 gttcggcgcg tacaagggca ccgaccgcga ggaagggctg cgctgggtgc tcccctggct
     4321 gacgcgcaag aagatcgcgg tgcgcgcgaa caacgtgatt tcggacaaga tcaaggtcaa
     4381 cgacctccgc ggcaatccga tcgagatggc ggcgcaggtc gtgtggcgcg tcaccgacac
     4441 cgcgcaggct ttgttcgacg tcgacgatta caaggaattc gtgatggcgc agatcgaggc
     4501 cgcggtgcgc tcgatcggct cgcgctaccc ctatgacgac atcgagcatc aggaagtcac
     4561 gctgcgcggc aaccacgacg aggtcggggt cgagctgcgc aaggcgctga tcgagcggct
     4621 gagcgtcgcg gggatcaccg tcgacgaatg cggcctcacc cacctcgcct atgcgcagga
     4681 aatcgccggc gcgatgctcc gccgtcagca ggccgaggcg gtgatcgcgg cgcgcaagaa
     4741 gctggtcgaa ggcgcggtga cgatggtcga gatggcgctg acccaattgt ccgaaaaggg
     4801 cgtcgtcgac ctcgacgacg agcgcaaggc ggtgatggtg tcgaacctga tggtcgtgct
     4861 gtgcggcgaa cgcgacacgc agccggtcgt gaa
//
LOCUS       DATMSU010000016         4873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_66789, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000016 DATMSU010000000
VERSION     DATMSU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66789"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VN034_00395"
     CDS             complement(<1..1044)
                     /locus_tag="VN034_00395"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWV59083.1"
                     /translation="MTAFKTLDDIGDVTGKRVLVRVDLNVPLFEGAVSDATRIQAAMP
                     TIRELSDKGAIVLLLAHFGRPKGAKNPTQSLSLVMGGVEDVLGHSVMFIPEAVGEGAK
                     AGIAVLAPGDVAVLENTRFYPGEEKNDPAFADALAEIGDLYVNDAFSAAHRAHGSTEG
                     IAHRLPAYAGRAMEAELKALDAALGNPAHPVAAVVGGAKVSSKIDVLRNLVAQVDHLI
                     IGGGMANTFLAARGVDVGKSLCEHDLVDTANAIFDAADAAGCTIHLPYDVVVSKEFAP
                     NPPTRTVNVHEVAADEMILDVGPAAVEALADVLKNCRTLVWNGPLGAFETPPFDTATV
                     ALARIAAALTREGS"
     gene            complement(1180..2187)
                     /gene="gap"
                     /locus_tag="VN034_00400"
     CDS             complement(1180..2187)
                     /gene="gap"
                     /locus_tag="VN034_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWV59084.1"
                     /translation="MAVKVAINGFGRIGRLVARAILERPDCGLELVAINDLADAKANA
                     LLFKRDSVHGPFPGEVSAEGDTMVVNGKAIRVTKERDPANLPHGELGVDIALECTGFF
                     TDAESAGKHLTAGAKRVLISAPAKGVDLTVVYGVNHDKLTGEHKIVSNASCTTNCLAP
                     VAKVLNDTLGIERGLMTTVHAYTNDQKILDQIHSDMRRARAAGMSIIPTTTGAARAVG
                     EVLPELKGKLDGSAIRVPTPNVSLVDLTFTPKRDTSVEEVNQILKDASEGALKGVLAY
                     SDEPLVSIDYNHCPASSTIDSLETSVLEGKLVRVVSWYDNEWGFSNRMVDTAGVIAKL
                     L"
     gene            complement(2225..4192)
                     /gene="tkt"
                     /locus_tag="VN034_00405"
     CDS             complement(2225..4192)
                     /gene="tkt"
                     /locus_tag="VN034_00405"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWV59085.1"
                     /translation="MTLPERQLANAIRALAMDAVQAANSGHPGMPMGMADVATVLYSD
                     YLKFDPSDPKWADRDRFVLSAGHGSMLAYATLNLAGYARPTLGDIRNFRQLGSPCAGH
                     PENFELEGVETTTGPLGQGLATAVGMAIAERHLNAIYGDDLVDHRTYVIAGDGCLMEG
                     INHEAIGLAGHLQLGRLTVLWDDNKITIDGSTDLSTSEDIAARYAATGWHVESCDGHD
                     PADIRRALDAALADPRPSLIACRTIIGFGAPNKQGTSATHGAPLGADEIAAARKELGR
                     TAEPFVIPGDISAAWAAFGEKGKKLHAEWNGRLASNEKKSDFEARLSGRIVPGDAFKA
                     YLDGLVAEPPKVATRKASENTLGALTADIASLIGGSADLTGSNNTKTSSTKPLTKDDY
                     SGRYIYYGIREFGMAAAMNGMALHGGVVPYGGTFLVFADYCRAAIRLSALQQQRVVYV
                     MTHDSIGLGEDGPTHQPIEHLQSLRAMPNLLVLRPADAVETAECWALALAQESRPSLL
                     ALTRQNLPPLRHDVTENLCLKGGYRLRDASAARKVVLVATGSEVSLAIEVADKLEAAG
                     HGADVVSMVSTELFDEQTAAYQADILPDDALIVSIEAGTTFGWERYTGRHGLRFGIDS
                     FGASAPIEDLFKHFGLTADAIAPQILARLND"
     gene            4350..4562
                     /locus_tag="VN034_00410"
     CDS             4350..4562
                     /locus_tag="VN034_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59086.1"
                     /translation="MELDLDESSLAIGRIERALSRIEKALADRANRPAPAPTVAMPAA
                     DQSALKAEVAAVIGELDRLIAEADRG"
     gene            4555..>4873
                     /locus_tag="VN034_00415"
     CDS             4555..>4873
                     /locus_tag="VN034_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWV59087.1"
                     /translation="MADVKLTIAGRPYDVHCADGQESQLVQLAAVVDEKARGIQGGTE
                     VRQLLFAALMLADEMQEARGKVEKTEPQSDSLRAAVALAESREAQARDELKAAVAREQ
                     DAMK"
BASE COUNT          806 a         1579 c         1624 g          864 t
ORIGIN      
        1 cgaaccttcg cgggtgagcg cggcggcgat cctggcgagc gcgacggtcg cggtgtcgaa
       61 cggcggcgtc tcgaacgcgc cgagcggccc gttccacacg agcgtgcggc aattcttgag
      121 cacgtccgcc aaagcttcaa ccgctgcggg accaacgtcg aggatcatct cgtcggcggc
      181 gacctcgtgg acgttgaccg tgcgcgtcgg cgggttcggg gcgaactcct tcgacaccac
      241 gacgtcatag ggcagatgga tcgtgcagcc cgcggcgtcg gccgcgtcga agatagcatt
      301 ggcagtgtcg acgagatcgt gctcgcagag cgacttgccg acatcgaccc cgcgtgcggc
      361 gaggaaggtg ttcgccatgc cgccgccgat gatcagatga tcgacctgcg cgacgaggtt
      421 cctcagcacg tcgatcttgc tcgacacctt agcgccgccg accactgccg cgaccggatg
      481 cgcgggattg ccgagcgcgg cgtcgagcgc cttgagttcg gcttccatcg cgcggccggc
      541 ataggcgggc aggcgatgcg cgataccctc ggtcgagcca tgcgcacggt gcgccgccga
      601 aaaggcgtcg ttgacgtaaa ggtcgccgat ctcggcgagc gcatcggcaa aggccgggtc
      661 gttcttttcc tcgcccggat agaaacgcgt gttttcgagc accgcgacgt cgccgggcgc
      721 aagcaccgcg atccccgcct tcgcgccctc gccgacggct tcggggatga acatcaccga
      781 atggccgagc acatcctcga ccccgcccat gacgaggctc agcgattggg tcgggttctt
      841 cgcccccttc ggccgcccga aatgggcgag gagcaggacg atcgcgccct tgtccgacag
      901 ttcgcgaatc gtcggcatcg ccgcctggat ccgcgtcgcg tcgctgaccg ccccctcgaa
      961 caacgggacg ttgaggtcga cgcgcacgag cacgcgcttg ccggtgacgt caccgatatc
     1021 gtcgagggtt ttgaacgcgg tcatcttttt cactccgttc ccggagtgcg cccctgcgaa
     1081 ggcaggggcc catctcctac gccatccaac tccacgaccg ttgcctcaag cccgtacggc
     1141 gggagatggg tccctgcctt cgcagggaca cggcaacaat tacagcagct tggcgatcac
     1201 gcccgcggtg tcgaccatgc ggttcgagaa gccccattca ttgtcgtacc agctgacgac
     1261 gcggacgagc ttgccttcga gcaccgaggt ttcgaggctg tcgatcgtcg agctcgccgg
     1321 gcagtggttg taatcgatcg aaacgagcgg ttcgtccgaa taggcaagaa cgcccttcag
     1381 cgcgccttcc gaggcgtcct tcaggatctg gttcacttcc tcgacgctcg tgtcgcgctt
     1441 cggcgtgaag gtcaggtcga ccaggctgac gttcggggtc ggaacccgga tcgccgaacc
     1501 gtcgagcttg cccttgagtt cgggcagcac ttcgccgacc gcgcgcgccg cgcccgtcgt
     1561 cgtcgggatg atcgacatgc ccgcggcgcg cgcgcggcgc atgtcgctgt ggatctggtc
     1621 gaggatcttc tggtcgttgg tataagcgtg gaccgtggtc atcagcccgc gctcgatgcc
     1681 gagcgtgtcg ttgagcacct tggcgaccgg cgcgaggcag ttggtcgtgc agctcgcgtt
     1741 cgacacgatc ttgtgctcgc cggtcagctt gtcgtggttg acgccataga cgacggtcag
     1801 gtcgacaccc ttcgccgggg ccgagatcag gacgcgcttg gcgccggcgg tcaggtgctt
     1861 gcccgcgctt tcggcgtcgg tgaagaaacc cgtgcattca agcgcgatgt cgacgcccag
     1921 ttcgccgtgc ggcaggttcg cggggtcgcg ttccttggtg acgcggatcg ccttgccgtt
     1981 gacgaccatc gtgtcgcctt cggccgaaac ctcacccggg aaaggaccat ggacgctgtc
     2041 gcgcttgaac aggagtgcgt tcgccttggc gtcggcgaga tcgttgatcg ccacgagctc
     2101 gagcccgcag tcggggcgtt cgaggatcgc gcgcgcgacg aggcggccga tgcgtccgaa
     2161 accgttgatt gcgactttca ctgccatgtt cggggagtcc tttctgcgaa tgggcggccg
     2221 gtccttaatc gttcagccgt gccaggatct ggggtgcgat agcatcggcg gtcaggccga
     2281 aatgcttgaa taggtcttca atcggtgccg aagcgccgaa gctgtcgatg ccgaagcgca
     2341 ggccgtggcg gccggtatag cgttcccagc cgaaggtcgt gcccgcctcg atcgagacga
     2401 tcagcgcatc gtcgggcagg atatcggcct gatacgccgc ggtctgttcg tcgaacaatt
     2461 cggtcgagac catcgagacg acgtcggcgc cgtggccggc ggcctcgagc ttgtcggcga
     2521 cttcgattgc cagcgacact tcggacccgg tcgcgacgag caccaccttg cgcgctgccg
     2581 aggcatcgcg aagacggtaa ccgcctttca ggcaaagatt ttccgtgaca tcatgacgca
     2641 gcggcgggag gttctgacgg gtcagcgcga gcagcgacgg cctgctttcc tgcgcgagcg
     2701 cgagcgccca gcattcggcg gtttcgaccg catcggcggg gcgcaggacg agcaggttcg
     2761 gcatcgcgcg cagcgactgg agatgctcga tcggctggtg cgtcgggccg tcttcgccaa
     2821 gcccgatgct gtcgtgcgtc atcacataga cgacgcgctg ctgctgcagc gccgacaggc
     2881 ggatcgcggc gcggcaatag tcggcgaaca caaggaaggt gccgccgtac ggcacgacgc
     2941 cgccgtgcag cgccatgcca ttcatcgccg ccgccatccc gaactcgcgg atgccataat
     3001 agatatagcg gcccgaataa tcatcttttg tcagtggctt ggtcgacgat gttttggtat
     3061 tgttcgaacc cgtgaggtcg gcgctgccgc cgatcagcga tgcgatatcg gcggtcagcg
     3121 cgccgagcgt gttttccgac gccttgcgcg tcgcgacctt cggcggttcg gcgacgaggc
     3181 catcgagata ggccttgaag gcgtcgcccg gaacgatcct gccgctcaga cgcgcttcga
     3241 aatcgctttt cttttcgttg cttgcaaggc gaccattcca ttcggcatga agtttcttgc
     3301 ccttctcgcc gaacgccgcc cacgccgcgg agatatcgcc gggaatcacg aaaggttcgg
     3361 cggtcctgcc gagttccttg cgcgccgccg cgatctcgtc ggcaccgagc ggggcgccgt
     3421 gcgtcgccga ggtgccctgc ttgttcggcg cgccgaagcc gatgatcgtc cggcacgcga
     3481 tcagcgacgg gcgcggatcg gcgagcgcag catcgagcgc gcggcgaata tcggcggggt
     3541 catggccgtc gcagctttcg acatgccagc cggtcgcggc atagcgcgcc gcgatgtcct
     3601 cgctggtcga cagatcggtc gacccgtcga tcgtgatctt gttgtcgtcc cacagcaccg
     3661 tcaggcgccc gagctgcaaa tgtccagcca aaccaatggc ttcgtggttg atgccttcca
     3721 tcaggcaccc gtcgccggca atgacatatg tgcgatgatc gaccagatcg tcgccataga
     3781 tcgcattcag atgccgctcg gcaatggcca tcccgaccgc ggtcgcgagc ccctggccga
     3841 gcggtcccgt cgtcgtctcg acgccttcga gctcgaaatt ctcgggatgc cctgcgcacg
     3901 ggctacccag ctggcgaaaa ttgcggatgt cgcccagcgt cggccgcgca tagcccgcga
     3961 ggttgagcgt cgcataggcg agcatcgagc cgtggcccgc cgagaggacg aatcggtcgc
     4021 ggtcggccca tttcggatca ctcggatcga acttcagata gtccgaataa agcacggtgg
     4081 cgacgtcggc catgcccatc ggcatcccgg gatggccgct gtttgcggcc tgtacggcgt
     4141 ccatcgcaag cgcacggatc gcgttggcga gctgacgttc gggcagtgtc atgagtcccc
     4201 cgggaaagga tgtgaagatg accggattcg gtggggacag ccctttgcgg gggcggggtc
     4261 aggagtcaac cgcctgcggc acaaattcgc gcgcatttcg gcgaggagag gcgattgcac
     4321 gccttcgcgc gcgctgctaa cccttggtca tggaactcga tctcgacgaa tccagcttgg
     4381 ccatcggccg tatcgaacgc gcgctgagcc gcatcgaaaa ggccttggcc gaccgggcga
     4441 accgccccgc gcccgccccc accgttgcca tgcccgccgc agaccagtca gcgctgaagg
     4501 ccgaggttgc cgccgtcatc ggcgaactcg accggctgat cgcggaggcc gaccgtggct
     4561 gacgtcaagc tgaccatcgc tggccgtccc tatgacgtcc attgcgccga cggccaggaa
     4621 tcgcagctgg tgcagctcgc cgcggtcgtc gacgaaaagg cgcgcggcat tcagggcgga
     4681 accgaagtgc gccaattgct gttcgcggcg cttatgctcg ccgacgagat gcaggaagcc
     4741 cgcggcaagg tcgaaaagac cgaaccccag tccgattcgc tacgcgccgc ggtcgcgctc
     4801 gccgagagcc gcgaggcgca ggcgcgcgac gagctgaagg cggccgtcgc gcgcgagcag
     4861 gacgcgatga agg
//
LOCUS       DATMSU010000017         9195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_180618,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000017 DATMSU010000000
VERSION     DATMSU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180618"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VN034_00420"
     CDS             <1..200
                     /locus_tag="VN034_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59088.1"
                     /translation="IASATTLIAGASITLPPILASMAYQWWRPAPFAIAVAIGALLLL
                     DHLVRPPSDPAPEPAIVHDER"
     gene            325..1518
                     /locus_tag="VN034_00425"
     CDS             325..1518
                     /locus_tag="VN034_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV59089.1"
                     /translation="MDRYLVISSDGHVGLPPERYRDYLEAKYHQQFDETVQLEIKARE
                     EHEKRFLIDDFNTKWRARVGDGLEGAWDGTIRNRVLNQDGVAAEVLFPDGITERNAPP
                     FGADIGLKPSPARAELQWAGARAHNRWLAEFCQDDPHRRIGLAVIPALYDLDETAKEI
                     EWARKNGLKGVFFPALTEGYDLYNHTKYHRIWAMLQDYNMPLHFHSGASPGYDTTQPG
                     WIGTYLCEFAFWMTRPLWSMTFGGVFEEYPQLKVCFTEAGGEFWFPWVMQLMDIRASA
                     KHTSGKLGDYYANMSMKPSEYFARNVWVGCSALPDEETTQAYYDIGVDRILWGTDYPH
                     PEGTWPSTLEKMTVSLGGLPDDDIQAMLGINALDVYDVDADALWKVAGEIGPRRELFV
                     KQAAE"
     gene            1614..2519
                     /locus_tag="VN034_00430"
     CDS             1614..2519
                     /locus_tag="VN034_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV59090.1"
                     /translation="MSSWRGIEEFLAVVEQGSFTAAGDHLGVSKSYISKTVHELEERL
                     GVQLLVRTTRRLSLTGAGESFHRECAELQTRLSEVEKQVGRYSTEPVGRLRVGLSDIF
                     GSDFMSALLAEFSTDHPGIVIEPIAYLNENEIVQERFDVVIRYGVLPNSNLRARTFGY
                     LSYCLCAAPQYVAARGWPATPQELIEHDCLTDLSATMSFNDEVSVKVGPRWMSNSGIA
                     LRSAARKGLGIASLPVSIVRQDLASGGMLALDAEWSFYDRKCWVVYSPGIMSVATRAF
                     IDYLVRQFSRVKVRPSMAASLAARY"
     gene            2578..3159
                     /locus_tag="VN034_00435"
     CDS             2578..3159
                     /locus_tag="VN034_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59091.1"
                     /translation="MVNTSLTYIVGALALAVSVPGCATPAFAQDESPSVAQLRDGSRD
                     FDWEIGSWTTHLRYLPEPLTGSTRWVEYRGTSEVRAILGGRANLVELSVEGPAGKIEG
                     VSLRLYNPKARQWTLNFANVRSGLLTSPVTGAFDGEGRGVFYGVDSVGDRTVLVRFLI
                     LDVGPNSARFEQAFSADGGANWETNWIAVDSRR"
     gene            3360..4346
                     /locus_tag="VN034_00440"
     CDS             3360..4346
                     /locus_tag="VN034_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWV59092.1"
                     /translation="MPIPYGWFGVGYGAELKVGDVRPVHYFGRDLVLFRNESGEAGLL
                     DAYCPHLGAHMGHGGQVEGDSLRCPFHHWAFRPDGFCSAIPYAKAFPPRAKREPLAKS
                     YPVAEKSGVIWAWYHPDDAAPMFDVIEYPEFTDPAFAEPTKREWRFASNPQEIAENGV
                     DVAHFAYVHAMDAVPEGETDYEGVRRRSVARGHRTIDLPTGERKQLPYSVETVQNGAG
                     QKFTRLSGLVELSLLVIATPVEADDVELRFCFTHPKVEPGSPQEKAIEAAIESTCGQK
                     GVEGDIPIWHNKIHRARPYLCDGDGPILRFRRYFEQFYADGPDGSRLVEAAE"
     gene            4415..5215
                     /locus_tag="VN034_00445"
     CDS             4415..5215
                     /locus_tag="VN034_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HWV59093.1"
                     /translation="MSRLRYVQGPVETRAPGMLKNTVYGIRATYETDPEVIAALLPQP
                     LVAVERPEIWLQMVHVAMHVTETDTVEIGALTVGVNCTHEGAPGAYCFHMAMEGETVV
                     TSGRERFGEPKKIAETHFERNGDHLRATCSRHGVAYFEIEGVIGEAIDAPLAFEEHLF
                     CYKGMPSIDTPGEFDGDVFLTRLNWQRNYSGRRAMTGTITLRESPYDPLADIPVRRLI
                     DMEYVEGGTATGGTLLRKVPGEWIAPHWVGRHDDPRNTGLELGARKAA"
     gene            5215..6084
                     /locus_tag="VN034_00450"
     CDS             5215..6084
                     /locus_tag="VN034_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59094.1"
                     /translation="MRDFSGRVAVVTGGASGVGKCLCADLARAGAHVVIADIDPARLE
                     AVRAEIDALGSGEVLGIACDVTKEASVRALADRVFDHFGTVHLLFNNAGVGLGEAQRK
                     IWTLPLSDWRWGIDVNVLGVVHGIAAFVPRMIEAGEEGVVINTSSTNGGLRSLPNTPI
                     YAATKAAVTSISEVLHQQLLREGGKLRAAVLFPGPHTVNTAIMASGEVRPADYVENEA
                     QTKVAYRTMEDLVRTTGLKLKLTEPDEVSAFALDGVRAGRFWLLPESGENDVLIAART
                     EQILARRDPPSAW"
     gene            6078..7415
                     /locus_tag="VN034_00455"
     CDS             6078..7415
                     /locus_tag="VN034_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59095.1"
                     /translation="MVTVAASDKPARESAPSRGVYRWLVVAILFVGYCFSAIDARVLT
                     LMVEPIQKDLGLSDFEISLLQGFAFSLLYSIAALPIGRFVDRTKRRATLIVWGVLFWS
                     AMTAACGFTSSFIGLFLARVGVGVGEATLSPTAYSLISDYFERRRRALAISFYAIGYP
                     IGGGLALLIGGWLLARFTKAGGATLPWLGSFEPWQMVFLCVAAPGLIVAALMMMIREP
                     ARQELAANLSVQSSLREGFDYISERWALFGSLIGSLALIALLAIGTALWFPTFLIRSY
                     GMSPAEVGMSYGMIMLTCGTIGTLSGGWFAGRLMQSGRADANMRVVLIATILKGLPLI
                     AAPLMPTAFLSLTMMAIGTLIGQASQGVMLTAIQDVTPNQLRGQVTAISLLCVNLIGT
                     GLGASVIAAITDFGFGDQAALGYSIAIAGAVVLPLIVALLLVALPHYRRAVEG"
     gene            7512..8990
                     /locus_tag="VN034_00460"
     CDS             7512..8990
                     /locus_tag="VN034_00460"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV59096.1"
                     /translation="MQRNLDLRYVVIGAGMSGILASIRLREAGHRNVTLLEKAHTVGG
                     TWRENRYPGLNCDVPAHAYTYSFAPNAEWSSYLAPGPEIQDYFEDVTARFDLREIAHF
                     GQEVDSCTYAGGVWTITTKAGMTVEADVVITATGVLHHPRMPEIEGLGSFAGTVAHSA
                     RWDESIALDGRRIGVVGSGSTGVQLVSALAARAGKLGHFQRSPQWIMPMPNIPYSEEQ
                     RAAFREDPALIDAIRYDPEYLANVLRFTEGIIKPDSEEMHIIETIVRDNLEQGVADPV
                     LREKLRPDYRAACKRLIFSPDYYQVVQQHDVDVVIEAIERIEPAGIRTRDGVLHELDL
                     IILATGFHADRFIRPAVVTGRGGASLDDMWAKHATAYNAISLPNFPNFFFLNGPTGPV
                     GNFSLIDVAERQWGYIEQLLDRVTGGECSEICVTADAMARYDQARTEAARGTIFASGC
                     SSWYLGKDGVPMTWPWSYGRFAEVMAAPDFADFEMVDAKLPA"
     gene            9017..>9195
                     /locus_tag="VN034_00465"
     CDS             9017..>9195
                     /locus_tag="VN034_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59097.1"
                     /translation="MTNPAVAVVTGASRGAGKGIAIGLGEKGMTVFVTGRSVDHSDAV
                     GWDGKPLAGTIHETAA"
BASE COUNT         1560 a         2926 c         3087 g         1622 t
ORIGIN      
        1 gcatcgcctc ggcgaccacc ttgatcgcgg gtgcgtcgat caccttgccg ccgatcctgg
       61 catcgatggc ctatcaatgg tggcggcccg cgcccttcgc gatcgcggtg gcgatcggcg
      121 cgctcctgct gctcgaccat ctggtccggc cgccgtctga cccggccccc gagccggcca
      181 ttgtccatga cgagcgatag tgcgttgccg ccgggccgct ttttctcgcg cgcgaagcgc
      241 atagtgtcgg gtcatggcgg tgcgcccggt ccggcttgaa ccggggcggc gcgcgcatct
      301 ggaataatga agcggagagg aaatatggac cgttatcttg tcatctcgtc ggacggccac
      361 gtcggcctcc cgcccgaacg ctatcgcgac tatctcgaag cgaaatatca ccagcagttc
      421 gacgagacgg tgcagctcga gatcaaggcg cgcgaagagc atgagaagcg cttcctgatc
      481 gacgatttca acaccaagtg gcgcgcccgc gtcggcgacg ggctcgaagg cgcgtgggac
      541 ggcacgatcc gcaaccgcgt gctgaaccag gatggcgtcg cggccgaagt gctcttcccc
      601 gacggcatca ccgaacgcaa cgcgccgccc ttcggtgccg acatcggatt gaagccgtcg
      661 cccgcgcgcg ccgagctgca atgggcgggc gcgcgggcgc acaaccgctg gctcgccgaa
      721 ttctgccagg acgatccgca ccgccgtatc ggcctcgccg tgatccccgc gctctacgat
      781 ctcgacgaga ccgcgaagga gatcgaatgg gcgcgcaaga acgggctcaa gggcgtgttc
      841 ttcccggcgc tgaccgaggg gtatgacctt tataaccaca ccaaatatca ccgcatctgg
      901 gcgatgctcc aggactataa catgccgctg catttccatt cgggggcatc gccggggtac
      961 gacacgacgc agcccggctg gatcggcacc tatctgtgcg aattcgcctt ctggatgacg
     1021 cggccgctct ggtcgatgac cttcggcggg gtgttcgagg aatatccgca gctcaaggtc
     1081 tgcttcaccg aggcgggcgg cgaattctgg ttcccgtggg tgatgcagct gatggacatt
     1141 cgcgcctcgg ccaagcacac gagcggcaag ctcggcgact attatgcgaa catgagcatg
     1201 aagccgagcg agtatttcgc gcgcaacgtc tgggtcggct gttcggcgct gcccgacgag
     1261 gaaacgacgc aggcctatta cgacatcggc gtcgaccgca tcctgtgggg caccgactat
     1321 ccgcaccccg aaggcacctg gccgagcacg ctcgaaaaga tgacggtcag cctcggcggc
     1381 ctgcccgacg acgatatcca ggcgatgctc gggatcaacg cgctcgacgt ttacgacgtc
     1441 gatgccgatg cgctgtggaa ggtcgcgggc gaaatcggcc cgcgccgcga actgttcgtc
     1501 aaacaggcgg cggaatgagc gaggcaagcc cttcccctta gggggagggg cttgtagtca
     1561 attgcattcg ggcgcctgct tgatagagca tcccggcctt ttgggatggg acgatgtcga
     1621 gctggcgtgg aatcgaggaa tttctggcgg ttgtcgagca gggcagtttc actgctgcgg
     1681 gcgatcatct gggagtttcc aaatcctaca tcagcaagac ggtgcacgaa ctggaagagc
     1741 ggctgggggt ccagctgctc gtccgcacca cgcgccgtct ctcgctgacc ggcgcgggcg
     1801 aaagcttcca tcgcgaatgc gccgagctgc aaacgcgcct cagcgaagtc gaaaagcagg
     1861 tcgggcgtta cagcaccgaa cccgtcggac gcttgcgcgt cggcctcagc gatattttcg
     1921 ggtccgactt catgtcggca ctgctcgccg aattcagcac cgaccatccg ggcatcgtga
     1981 tcgagccgat cgcctatctc aacgagaacg agatcgtgca ggagcggttc gacgtcgtca
     2041 tccgatacgg cgtgctgccc aattcgaacc tgcgcgcgcg gaccttcggc tatctgtcct
     2101 attgcctgtg cgccgcgccc caatatgtcg ccgcgcgcgg ctggcccgcg acgccgcagg
     2161 agctgatcga gcacgactgc ctgaccgacc tcagcgcgac gatgagcttc aacgacgagg
     2221 tcagcgtcaa ggtgggtcca cgctggatga gcaacagcgg catcgcgctg cgcagcgcgg
     2281 cgcgcaaggg gctcggcatc gcgagcctgc cggtcagcat cgtccgccag gacctcgcga
     2341 gcggcgggat gctcgcgctc gacgccgaat ggtcctttta cgaccgcaaa tgctgggtcg
     2401 tctattcgcc ggggatcatg tcggtcgcga cgcgcgcctt catcgattat ctggtccgcc
     2461 aattctcgcg cgtgaaggtt cgcccgtcga tggcggcatc gctcgcggcg cgatattaag
     2521 ggcacttcag ttaccgacga gccgggccgc atattgtctg acgagaggga gagagccatg
     2581 gtgaacacgt cgctgacgta tatcgtgggc gcgcttgcct tggctgtctc ggttcccggc
     2641 tgcgccacac cagccttcgc ccaggatgaa tcgccaagcg tagcgcaact gcgcgacggg
     2701 agccgcgatt tcgattggga aatcggaagc tggacgacgc atctccgata tcttccggag
     2761 ccgctgaccg gatcgacgcg ctgggtcgaa tatcgcggca caagcgaggt tcgggccatt
     2821 ctcggcggcc gcgccaatct cgtcgagctt tcggttgaag ggccagcggg caagatcgag
     2881 ggtgtttcgc tccgactcta taatccgaaa gcacgccaat ggacgctcaa ctttgcgaat
     2941 gtccgcagcg gactcctcac ttcgccggtg accggcgcgt tcgatggcga gggtcggggt
     3001 gtcttctatg gtgtggacag tgtcggcgat cggaccgtgc ttgtccgctt cctgatcctg
     3061 gacgtcgggc cgaactccgc gcgctttgaa caggcgtttt cggccgacgg cggggcgaac
     3121 tgggaaacca actggatcgc ggtggacagc cggcgctgac acgcaagctt tgccaaagct
     3181 ccgccggcct ggccaaaggc tggtgtccaa cagatcgcca ttgttcgtca ccgcgcacaa
     3241 actgtgcgcc atttgggtct tacctcgagc gcggcaatcg atacactcgc ctcacaagag
     3301 ccgcccggca aggacggcca tccgggagac tgacgttgcc aaaggttgaa cgctttccga
     3361 tgcccatccc atacggctgg ttcggtgtcg gctatggcgc cgagctgaag gtcggcgatg
     3421 tgcggccggt ccattatttc gggcgcgacc tcgtgctctt ccgcaatgaa agcggcgagg
     3481 cggggctgct cgatgcctat tgcccgcacc ttggtgcgca tatggggcac ggcgggcagg
     3541 tcgagggcga cagcctgcgc tgtcccttcc atcactgggc gttccgtccc gacggcttct
     3601 gcagcgcgat cccctatgcc aaggcctttc cgccgcgcgc caagcgcgag ccgctcgcca
     3661 aatcctatcc ggttgccgaa aagagcggcg tcatctgggc ctggtatcat cctgacgatg
     3721 cggcgccgat gttcgacgtc atcgaatatc ccgaattcac cgacccggcc tttgccgaac
     3781 cgacgaagcg cgaatggcgc tttgccagca acccgcagga aattgccgaa aacggcgtcg
     3841 acgtcgcgca ttttgcctat gtccatgcga tggatgcggt gcccgagggc gagaccgatt
     3901 atgagggcgt gcggcggcgc agcgttgcgc ggggtcaccg cacgatcgac ctgccgacgg
     3961 gcgagcgcaa gcagctccct tattcggtcg agacggtcca gaacggcgcg ggacagaagt
     4021 tcacgcggct gagcgggctc gtcgaactgt cgctgctcgt cattgcgacc ccggtcgagg
     4081 ccgacgatgt cgagctgcgc ttctgcttca cccatcccaa ggtcgagccc ggatcgccgc
     4141 aggaaaaggc gatcgaggcg gcgatcgaga gcacctgcgg ccagaagggc gtcgagggcg
     4201 acattccgat ctggcacaac aagatccacc gcgcgcggcc ctatttgtgc gacggcgacg
     4261 gcccgatcct gcgcttccgc cgctatttcg agcaatttta tgccgacggg cccgacggca
     4321 gccgcctcgt cgaagcggcc gaataacaac ccattttcgt tccatgcgga tcgatccgct
     4381 caccgcgcct gcgggcgcca tcgggagaat tgcgatgtcg agattgcgtt atgtccaggg
     4441 acccgtcgaa acgcgcgcgc cggggatgct caagaacacc gtctacggaa tccgggcgac
     4501 ctatgaaacc gatcccgagg tgatcgcggc gctgctgccg cagccgctcg tggcggtcga
     4561 gcggcccgaa atctggctgc agatggtcca tgtcgcgatg cacgtcaccg agaccgacac
     4621 ggtcgagatc ggcgcgctga ccgtcggggt gaactgcacg cacgagggcg cgccgggcgc
     4681 ctattgcttc cacatggcga tggagggcga aaccgtcgtc acttcgggcc gcgagcgctt
     4741 cggcgaaccg aagaagatcg cggagacgca tttcgaacgc aacggcgacc atctgcgcgc
     4801 gacctgctcg cgccacggcg tcgcctattt cgagatcgag ggggtgattg gcgaggcgat
     4861 cgacgcgccg ctggcgttcg aggagcatct tttctgctac aagggcatgc cgtcgatcga
     4921 cacgcccggc gagttcgacg gcgacgtctt cctcacccgc ctcaactggc agcgcaacta
     4981 tagcggccgc cgcgcgatga cggggacgat cacgctccgc gaatcgccgt acgatccgct
     5041 cgccgacatt ccggttcgcc gcctgatcga catggaatat gtcgagggcg gcaccgcgac
     5101 cggcggcacg ctgctgcgca aggtgcccgg cgaatggatc gcgccgcact gggtcgggcg
     5161 tcacgacgac ccacgcaaca ccggtctcga actcggcgcg cggaaggccg cctgatgcgg
     5221 gatttttcgg gccgggtggc ggtcgttacc ggcggggcga gcggggtcgg caaatgcctt
     5281 tgcgccgacc tcgcgcgcgc cggtgcgcat gtcgtgatcg ccgatatcga tccggcacgg
     5341 ctcgaagcgg tgcgtgcgga gatcgacgcg ctgggcagcg gcgaggtgct cggaatcgcg
     5401 tgcgacgtca cgaaggaggc gtcggtgcgc gcgctcgcag accgggtgtt cgatcatttc
     5461 ggcacggtgc atctgctctt caacaatgcc ggcgtcggcc tcggcgaggc gcagcgcaaa
     5521 atctggacgc tgccgctcag cgactggcgc tgggggatcg acgtcaatgt gctcggcgtc
     5581 gtccacggca ttgcggcctt cgtgccgcgg atgatcgagg cgggcgagga gggggtggtg
     5641 atcaacacca gctcgaccaa cggcgggctg cgctcgcttc ccaacacgcc gatctatgcc
     5701 gcgaccaagg ccgcggtcac cagcatctcc gaggtgctgc accagcagct tttgcgcgag
     5761 ggcggcaagc tgcgcgccgc ggtgctcttc cccggtccgc acacggtcaa caccgcgatc
     5821 atggcgtcgg gcgaggtgcg gccggcggat tatgtcgaga atgaagcgca gacgaaggtc
     5881 gcctatcgca cgatggagga tctggtgcgc accaccggcc tcaagctcaa actgaccgag
     5941 cccgacgagg tttcggcctt cgcgcttgac ggcgtgcgcg cggggcgctt ctggctgctg
     6001 cccgagagcg gggagaatga tgtgttgatc gcggcgcgga ccgaacagat tctggcgcgg
     6061 cgggatccgc cctcggcatg gtgaccgtcg cggcgtccga taaacccgcg cgcgagtccg
     6121 cgccctcgcg cggcgtctat cgctggctcg tcgtcgcgat cctgttcgtc ggctattgct
     6181 tcagcgcgat cgacgcgcgc gtactgaccc tgatggtcga gccgatccag aaggacctcg
     6241 ggctcagcga tttcgagatc agcctgcttc agggcttcgc tttctccttg ctctacagca
     6301 tcgccgcgct gcccatcggc cgctttgtcg accgcaccaa aaggcgcgcg acactcatcg
     6361 tctggggcgt gctgttctgg tccgcgatga ccgccgcgtg cggctttacc agcagcttca
     6421 tcggcctgtt cctcgcgcgc gtcggcgtcg gggtgggcga agcgacgctc agcccgaccg
     6481 cctattcgct gatcagcgac tatttcgagc ggcgccggcg ggcgctggcg atcagtttct
     6541 acgcgatcgg ctatccgatc ggcggcggcc tcgcgctttt gatcggcggc tggctgctcg
     6601 cgcggttcac caaagccggc ggcgcgacat tgccatggct cggcagtttt gaaccgtggc
     6661 agatggtgtt cctgtgcgtt gcggcgcccg gattgatcgt cgccgcgctg atgatgatga
     6721 tccgcgagcc ggcgcgacag gaactggcgg cgaacctgtc ggtgcaaagc agcctgcgcg
     6781 aggggttcga ctatatttcc gagcgctggg cgctgttcgg ctcgctgatc ggttcgctcg
     6841 cgctgatcgc ccttctggcg atcggcaccg cgctgtggtt tccgaccttc ctcatccgca
     6901 gctatggcat gagccccgcc gaagtcggga tgtcttacgg gatgatcatg ctgacctgcg
     6961 gcaccatcgg cacgctgtcg ggcggctggt tcgcggggcg gctgatgcaa agcggccgcg
     7021 ccgacgcgaa catgcgcgtc gtgctgatcg cgacgatcct caagggcctg ccgctgatcg
     7081 ccgcgccgct gatgccgacg gccttcctgt cgctgacgat gatggcgatc ggcacgctga
     7141 tcgggcaggc gtcgcagggg gtgatgctca ccgcgattca ggatgtcacg ccgaaccagc
     7201 tgcgcggtca ggtcaccgcg atctcgctgc tctgcgtcaa tttgatcggc accgggctcg
     7261 gcgcgagcgt catcgccgcg atcaccgatt tcggtttcgg cgaccaggcg gcgctcggct
     7321 attcgatcgc gatcgccggc gcggtcgtgt tgccgctgat cgtcgcgctg ctgctcgtcg
     7381 cgctgccgca ctatcggcgc gcggtcgagg gctgacgccg ccatacgata tggtccgttt
     7441 gagtgatgtc gggcccggcg gggctgccta gggaaggagg aatagcgaaa caatcaagcg
     7501 gagtggacag cgtgcagcgc aatctggatt tgcgatatgt cgtcatcggg gcgggcatgt
     7561 cggggatatt ggcgagcata aggctgcgcg aggcggggca tcgcaacgtc accctgctcg
     7621 aaaaggcgca tacggtcggc ggcacctggc gcgaaaaccg ttatccgggg ctcaattgcg
     7681 acgtgcccgc gcacgcctat acctacagtt tcgcgccaaa cgccgaatgg agcagctatt
     7741 tggcgcccgg gcccgagatt caggattatt tcgaggacgt cacggcccgc ttcgacctgc
     7801 gcgagatcgc gcatttcggg caggaggtcg acagctgcac ttatgcgggc ggcgtctgga
     7861 cgatcacgac caaggcgggg atgaccgtcg aggccgacgt cgtgatcacc gcgacgggcg
     7921 ttctccatca tccgcggatg cccgagatcg aagggctggg cagcttcgcc ggcaccgtgg
     7981 cgcacagcgc gcgctgggat gagagcattg cgctcgacgg gcggcggatc ggcgtcgtcg
     8041 gcagcgggtc gacgggggtg cagctcgtct cggcgctcgc ggcgcgcgcg ggcaagctcg
     8101 gtcatttcca gcgctcgccg caatggatca tgccgatgcc gaacatcccc tatagcgagg
     8161 agcagcgcgc ggcgttccgc gaggatccgg cgctgatcga cgcgatccgc tacgaccccg
     8221 agtatctggc gaatgtgctg cgtttcaccg agggcatcat caagcccgac agcgaggaaa
     8281 tgcacatcat cgagacgatc gtgcgcgaca atctggagca gggcgtcgcc gatccggtgc
     8341 tgcgcgaaaa gctgcgtccc gattaccgcg cggcgtgcaa gcggctgatc ttctcgcccg
     8401 actattatca ggtcgtgcag cagcatgacg tcgatgtcgt gatcgaggcg atcgagcgga
     8461 tcgagcccgc gggcatccgc acccgcgacg gggtgctcca cgaactcgac ctgatcatcc
     8521 tcgcgacggg tttccacgcc gaccgcttca tccggccggc ggtggtgacg ggacgcggcg
     8581 gggcgtcgct cgacgacatg tgggcgaagc acgcgaccgc gtacaacgcg atctcgctgc
     8641 ccaatttccc gaacttcttc ttcctaaacg ggccgacggg gccggtcggc aatttctcgc
     8701 tgatcgacgt cgccgagcgc caatggggct atatcgagca gctgctcgac cgggtgacgg
     8761 gcggcgaatg ctcggaaatc tgcgtcaccg ccgatgcgat ggcgcgctat gatcaagcgc
     8821 gcaccgaggc cgcccggggc acgatcttcg cctcgggctg ttcgagctgg tatctcggca
     8881 aggacggcgt gccgatgacc tggccgtgga gttatggccg cttcgccgaa gtgatggcgg
     8941 cgcccgattt cgccgatttc gagatggtgg acgccaagct tccggcctga cggggcgcgc
     9001 attcgaggag acgcatatga cgaatccggc agtcgccgtc gtgacgggcg ccagccgcgg
     9061 cgcgggcaag ggcatcgcga tcgggctcgg cgaaaagggc atgacggtgt tcgtcaccgg
     9121 gcgcagcgtc gaccatagcg acgcggtcgg ctgggacggc aagccgctcg ccggcacgat
     9181 ccacgaaacc gcggc
//
LOCUS       DATMSU010000018         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_104806,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000018 DATMSU010000000
VERSION     DATMSU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104806"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VN034_00470"
     CDS             complement(<1..1451)
                     /locus_tag="VN034_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWV59098.1"
                     /translation="MDSVAAFIAEQKAVIGLFVLAGMFAAFVWERFSPSVVAVAGAGT
                     MLALGLLDEKGAYGVFSNSAPLTVGAMFILSGALIRTGVIDKVGNYVVDRAKRHPRLA
                     VVEVFGGGLLVSGFINSTPFVVVMMPVCFRLAEALGVSPKKLLMPLSVIAVLGGCLTL
                     IGTSTNLVVSGIAEQAGLEPFGLFDITPYGLAVAAAGVVGLLVMARFLPSDYPRIAEK
                     ADGAEQLYLTELGLRDDDAAIDMALDAANLGIAPSQIVGVIAGGRMITGNDAQHHRLA
                     AGNRIVARLDGAALMTLRSEGRFAIGIGDSPERESTVVEVTVSPSHRSIDRPLAEIPF
                     LQRQRVRILGVTRFRHLPGPTLSENRIRAADRLLIDADEANIAQLRDNPNLIGLAMAS
                     VTAFRRRRAWIAILVMACAVLLSATDVMSIGIAAFIGVAIVLLTRCIDASEAWGSIDG
                     DVLILIFAMLAVGAALEQAGSVALMVGALDPVLA"
     gene            complement(1459..>1819)
                     /locus_tag="VN034_00475"
     CDS             complement(1459..>1819)
                     /locus_tag="VN034_00475"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWV59099.1"
                     /translation="KAVEIGAGFHAARLRGEENADPMRPAADGSNRPDFLSNNAGGIA
                     GGISTGQPVVVRVAFKPTSSILTPVPTVNKAGEATDIVTKGRHDPCVGIRGAPVVEAM
                     MALVLADHKLLHRAQNG"
BASE COUNT          332 a          618 c          611 g          258 t
ORIGIN      
        1 gccaggaccg ggtcgagcgc gccgaccatc agcgcgaccg accccgcctg ttcgagcgcc
       61 gcgccgacgg cgagcatcgc gaagatcagg atcagcacat cgccgtcgat gctgccccac
      121 gcctccgacg cgtcaatgca gcgggtgagc agcacgatgg cgacgccgat gaaggcggcg
      181 atgccgatcg acatcacgtc ggtcgccgag agcaggaccg cgcaggccat cacgaggatc
      241 gcgatccacg cgcggcggcg gcggaaggcg gtgaccgacg ccatcgccag cccgatcaga
      301 ttggggttgt cgcgcagctg cgcgatattc gcctcgtcgg cgtcgatcag caggcggtcg
      361 gcggcgcgga tgcggttttc ggacagcgtc ggccccggaa ggtggcgaaa gcgggtgacg
      421 ccgaggatgc ggacgcgctg ccgctgaagg aaggggattt cggcgagcgg ccggtcgatc
      481 gagcggtggc tcggcgaaac cgttacctcg accacggtcg attcccgctc gggactatcg
      541 ccgatgccga tcgcaaagcg tccttcgctg cgcagcgtca tcagcgccgc gccgtcgagc
      601 cgcgcgacga tccggttgcc tgccgcgagc cggtggtgtt gcgcgtcgtt cccggtgatc
      661 atgcgcccac ccgcgatcac gccgacaatc tgcgatggcg cgatgccgag attggccgcg
      721 tcgagggcca tgtcgatggc ggcatcatcg tcccgaaggc cgagttcggt caggtaaagc
      781 tgctccgccc catcggcctt ttcagcgatc cgcggatagt ccgacggcag gaagcgcgcc
      841 atcacgagca gacccacgac ccccgccgcc gcgaccgcga ggccataagg cgtgatgtcg
      901 aacagaccga agggttcgag ccccgcctgt tcggcgatgc ccgacacgac gaggttggtc
      961 gaggtgccga tcagggtcag gcagccgccg agcaccgcga tcaccgacag cggcatcagc
     1021 aatttcttgg gcgagacccc gagcgcctcg gcgagccgga aacagacggg catcatcacg
     1081 acgacgaagg gcgtgctgtt gatgaagccc gacacgagca gcccgccgcc gaacacttcg
     1141 accaccgcga ggcgcgggtg ccgcttggcg cggtcgacga cataattgcc gaccttgtcg
     1201 atcaccccgg tacggatcag cgcgcccgag aggatgaaca tcgccccgac agtgagcggc
     1261 gcgctgttcg agaaaacgcc ataagcaccc ttttcgtcga gaaggccgag cgcaagcatc
     1321 gtgcccgcgc cggcgaccgc gaccaccgaa ggcgagaagc gttcccagac aaatgccgca
     1381 aacatgcccg cgagcacgaa cagaccgata accgcctttt gctccgcgat gaaggcggcg
     1441 acggagtcca tcaggccatc agccgttttg tgccctgtgc agcagcttgt gatcggcgag
     1501 gacgagcgcc atcatcgctt cgaccaccgg cgcaccgcgg atgccgacgc acggatcatg
     1561 gcggcccttg gtgacgatat cggtcgcctc acccgccttg ttcaccgtcg gcacgggagt
     1621 caggatcgag ctggtcggct tgaacgcgac gcgtacgacg accggctggc cggtcgagat
     1681 cccccccgcg atgccacccg cattattcga caggaaatcg gggcggttgc tgccgtcggc
     1741 cgcggggcgc atcgggtcgg cattttcctc accgcgaagc cgcgccgcgt ggaagccggc
     1801 accgatctcg accgccttc
//
LOCUS       DATMSU010000019         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_85836, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000019 DATMSU010000000
VERSION     DATMSU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85836"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VN034_00480"
     CDS             <1..377
                     /locus_tag="VN034_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWV59100.1"
                     /translation="IALAPLILLPPRDWLRTRCDARLVEMFAGGAIAEVETLLARDLD
                     PDLPAMRAIGVPQIAAFLRGEISRAEALEAAQAATRQYAKRQYTWFRHQPPADWPRHE
                     ESLNIDSVDKLAIILRDRLLTG"
     gene            452..2206
                     /gene="ilvB"
                     /locus_tag="VN034_00485"
     CDS             452..2206
                     /gene="ilvB"
                     /locus_tag="VN034_00485"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HWV59101.1"
                     /translation="MTEMSGADMVVQALVDLGVDTVFGYPGGAVLPIYDALYKHPTIK
                     HILVRHEQAATHAAEGYARSTGKPGVVLVTSGPGATNAVTGITDALLDSIPMVVLTGQ
                     VSTALIGTDAFQECDTVGITRHCTKHNYLVMDPDRLGAILHEAFHIATHGRPGPVVID
                     IPKNVQVATGEYVVPEKILHNSYRPQVEPDADAISQAIDMIAAAERPVFYTGGGVINA
                     GPDASAALRQLVSLTGAPITSTLMGLGAFPSDDDKWLGMLGMHGTYEANMAMNRADLI
                     IAVGARFDDRVTGRLDAFSPDSKKIHIDIDRSSINKIVPVDLAIVGDAGRALDALIAA
                     WSDKGHKARDLGEWWRRVDGWRATRCLDFPEKKEAGADIMPQRAVKALFDATRGRDPI
                     ITTEVGQHQMWAAQHFGFSAPNRWLTSGGLGTMGYGFPAAVGAQIANPDRLVICIAGE
                     ASLQMNIQEMGTVAQYRLPVKIFILNNEWMGMVRQWQELTYESRYSNSYSDSLPDFVK
                     LADAYGWTGLRIDTLGELEAGIQTMIDTPGPVIVDCRVAKLANCFPMIPSGAAHTDMI
                     LQPNDITGTMDDEAKALV"
     gene            2370..2888
                     /gene="ilvN"
                     /locus_tag="VN034_00490"
     CDS             2370..2888
                     /gene="ilvN"
                     /locus_tag="VN034_00490"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWV59102.1"
                     /translation="MKIKTEAGERHVLAITVDNEAGVLAKITGLFTARGYNIESLTVA
                     DITADHALSRITIVTSGPPPIIDQIIAQLDRLVPVHKVVDLNDAGEAHVEREIALVKV
                     AGTGDKRIEALRMADVFRAKVVDTTLSSFIFEITGTSDKVDRFIALMRECGLVEVGRT
                     GVVAIARGSEAL"
BASE COUNT          540 a         1011 c          880 g          466 t
ORIGIN      
        1 acatcgccct cgccccgctg atcctcctgc ccccgcgcga ctggctccgc acccgctgcg
       61 acgcccgcct cgtcgagatg ttcgcgggcg gcgccatcgc agaggtcgaa accctgctcg
      121 cgcgcgatct cgaccccgat cttcccgcga tgcgcgcgat cggggtgccg cagatcgccg
      181 cttttctccg cggcgaaatc agccgcgccg aggcgctcga agcggcgcaa gccgctaccc
      241 gccaatatgc aaagcgccaa tatacgtggt tccgccacca gccacccgcc gactggccgc
      301 gccacgaaga gtcattaaac atcgattctg ttgacaaatt agcaataata ttacgtgata
      361 gactgttgac aggatagaat catacaccta ttgcccgcaa ccctgttgca gcgcacaagc
      421 gctgcgtttt tgcgttgaaa ggagtttcgt cgtgacggaa atgagcggtg ccgacatggt
      481 ggttcaggcg ctggtcgacc tcggggtcga tacggtgttc ggctatccgg gcggcgcggt
      541 ccttcccatc tatgacgcgc tctacaagca cccgacgatc aagcatattc tcgtccgcca
      601 cgaacaggcg gcgacgcacg cggccgaagg ctatgcgcgc tcgaccggca aacccggcgt
      661 cgtgctcgtc acctcgggcc ccggcgcgac caacgccgtc accggcatca ccgacgcttt
      721 gctcgactcg atcccgatgg tcgtgctgac gggccaggtg tcgaccgcgc tgatcggcac
      781 cgacgcgttc caggaatgcg acaccgtcgg catcacgcgc cattgcacca agcataatta
      841 tctcgtgatg gaccccgacc gcctcggcgc catcctgcac gaggcgttcc acatcgcgac
      901 gcacggccgc cccggcccgg tcgtgatcga catcccgaag aatgtgcagg tcgcgacggg
      961 cgaatatgtc gtccccgaaa agatcctgca caacagctat cgcccgcagg tcgaacccga
     1021 cgccgacgcg atcagccaag cgatcgacat gatcgccgcg gccgaacgcc cggtcttcta
     1081 caccggcggc ggcgtgatca acgcggggcc cgatgccagt gctgctttgc gccagctcgt
     1141 cagcctgacc ggcgcgccga tcacctcgac cttgatgggc ctcggcgcct ttccgtcgga
     1201 cgacgacaaa tggctcggca tgctcggcat gcacggcacc tatgaagcca atatggcgat
     1261 gaaccgcgcc gacctcatca tcgcggtcgg cgcgcgcttc gacgaccgcg tcaccggccg
     1321 tctcgacgcc ttttcgcccg attcaaagaa gatccacatc gacatcgacc gcagctcgat
     1381 caacaagatc gtgcccgtcg atctcgcgat cgtcggcgac gcgggccgcg cgctcgatgc
     1441 gctgatcgcc gcatggtccg acaaggggca caaggcgcgc gacctcggcg aatggtggcg
     1501 ccgcgtcgac ggctggcgcg cgacgcgctg cctcgacttc cccgaaaaga aggaggcggg
     1561 cgccgacatc atgccgcagc gcgcggtgaa ggccttgttc gacgcgacgc gcggccgcga
     1621 cccgatcatc acgaccgagg tcggccagca ccagatgtgg gcggcgcagc atttcggctt
     1681 ttcggcgccc aaccgctggc tcacctcggg cgggctcggc acgatgggtt acggctttcc
     1741 cgccgcggtc ggcgcgcaga tcgccaaccc cgaccgcctc gtcatctgca tcgcgggcga
     1801 agcctcactc cagatgaaca tccaggaaat gggcacggtc gcgcaatacc gcctgccggt
     1861 gaagatcttc atcctcaaca atgaatggat ggggatggtc cgccagtggc aggaactgac
     1921 gtacgagagc cgctattcga acagctattc ggacagcctg cccgatttcg tgaagctcgc
     1981 cgacgcttac ggctggacgg gcctgcgcat cgacacgctg ggcgaactcg aagcgggcat
     2041 ccagaccatg atcgacacac ccggaccggt gatcgtcgac tgccgcgtcg ccaagctcgc
     2101 caactgcttc ccgatgatcc cgtcgggcgc cgcgcacacc gacatgatcc tccagccgaa
     2161 cgacatcacc ggcacgatgg acgacgaagc gaaagcgctg gtgtgatgat ggcgaacgct
     2221 tttcatcccc tcccgcaagc gggaggggca gcgaggcttg cgaacttgtt cgctagccgc
     2281 agcggggtgg gcatcgccac gccgtctgct cgcttcgctc gcgcccaccc ctcgatcccc
     2341 tcccgcctgc gggaggggaa ggaataacca tgaaaataaa gaccgaagcc ggcgagcgcc
     2401 atgtgcttgc catcaccgtc gataacgagg ccggggtgct cgccaagatc accgggctgt
     2461 tcaccgcgcg cggctataat atcgaaagcc tgacggtggc cgacatcacg gccgaccatg
     2521 cgctgtcgcg tatcaccatc gtcacctcgg gcccgccgcc gatcatcgac cagatcatcg
     2581 cgcagcttga ccggctcgtg cccgtccaca aggtcgtcga cctcaacgac gcgggcgagg
     2641 cgcatgtcga acgcgagatc gcgctcgtga aggtcgcggg caccggcgac aagcggatcg
     2701 aggcgctgcg catggccgac gtcttccgcg ccaaggtcgt cgacacgacg ctgtcgagct
     2761 tcatcttcga aatcaccggg acgagcgaca aggtcgaccg cttcatcgcg ctgatgcgcg
     2821 aatgcgggct ggtcgaggtc ggccgcaccg gcgtcgtcgc catcgcccgc gggtcggagg
     2881 cgctctgagt caccgtc
//
LOCUS       DATMSU010000020         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_180655,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000020 DATMSU010000000
VERSION     DATMSU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180655"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VN034_00495"
     CDS             <1..1288
                     /locus_tag="VN034_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HWV59103.1"
                     /translation="LAAAIGTAALAGLLFGFDTAVIAGVTSDLTRLFALTPETLGVTV
                     SAALWGTLVGALFAGKPGDAFGSRDSLRVLAILYFVAGLGCALAPSWTAFLVFRFICG
                     LAIGGSSVLAPVYIAEIAPPRHRGFLVGLFQLMIVTGILVAYLSNATIAGLIGGETAW
                     RWKLGVTAAPALLFFVLLFAIPNSPRWLLAKGRRDEAGMAMTRIGAPTAELDRIETAL
                     ERDPPGERLSWRQHRRPMLLAILIAMFNQLAGINAVLYYLNDIFARAGSLSPDRQAVM
                     IGIANLVFTLAGMALIDRLGRKTLLLAGAAGMTLCLAAAAGVLFGVLDNALMLPALIG
                     FIAFFATSQGAVIWVYISEIFPTPVRARGSALGASTHWLMNALIAGIFPVLVAWSPGA
                     PFVIFAAAMVVQFVVVAAVFPETKGVDLDDMAQRID"
     gene            complement(1295..2536)
                     /locus_tag="VN034_00500"
     CDS             complement(1295..2536)
                     /locus_tag="VN034_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWV59104.1"
                     /translation="MSLNLPGRDRRQSATSGPEARDARLTLSLSGTNLERAGDYNQRI
                     VLQAIRLTEETTRSDLARVTGLTPPTIVNITKRLIDLGLVKPAGRLQGKRGQPAMRIV
                     IDPDGAFAIGLNIDRDHITLVTLDLAGKIRSRHTREIAFALPGTVVDHVRDILPGLLE
                     EGGVDRARILGVGIALPDDLGRIALPHRPKEYDAWDAIDLGALLGEVLPWPLHADNDA
                     ASAALGEVHLGDGIALPSFFYLLISAGLGGGLVIDRSYVRGADSRSGEIWAMPDPERG
                     DGANVQDTVSLSALYARLEAEGHDVDAPDALLGSSPDREAVILKWLDDAADALVQPLI
                     AVNCLINPAAILIGGRLPGKLVDGLVERLNARMTGLQLPAVAPVRRAGAADDASAIGA
                     AIIPFLDHILPSESILMQAGR"
     gene            complement(2678..4486)
                     /locus_tag="VN034_00505"
     CDS             complement(2678..4486)
                     /locus_tag="VN034_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWV59105.1"
                     /translation="MRFAIALMLMPLLPLAVLPAPAMAETAPAAAQDEALLRFLDDAF
                     DARIALQPESQTHLGLKTNYDRLDDYTDAAAVREQALIEQQLKEMKARFRPEQLGASA
                     RVSYRLFEYDVERRRASFPFRKLRFPVTTNGSPAGDIPVLLINNHKVDSVADAEAYIA
                     RLRDTDRVMGEVAATMREQAAAGAVPNKVNFAPARADAKKILVGAPFDGGADSTLLAD
                     FRKKVGALDAPDAVKAKLIADATAALTGPFKHGYDVLIAAIDEIEPQSKGNFGVWSLP
                     GGAAYYADRLKNSTTTDLTADQIHELGLKQVAAIREEMEAIKREVGFKGTLEQFFDHI
                     RTDPKLKYPNTAAGREAYLTDARAVIAAAMAAAPRYFSVLPKAALEVRAVEKWREATA
                     STAFYNPPSADGTRPGIYYVNLVDMNQTQKVQVAAIAAHEGAPGHHFQIARQQELAGI
                     PKFRKFGGYGAYIEGWGLYSERLANEMGIYKTPYDRFGMLSLQVWRAIRLVLDTGIHS
                     KRWTREQAIAYFRANSSVSDTDIAREVDRYFNWPGQATSYMVGQLKIAELRERAEREL
                     GARFDIREFHEAVLGEGALPLSILEEQVAAYIASKR"
BASE COUNT          710 a         1604 c         1544 g          845 t
ORIGIN      
        1 cctcgctgcg gcgatcggga cggcggcgct cgccgggctc ctcttcggat tcgatacggc
       61 ggtcatcgcg ggcgtgacga gcgacctcac ccgcctgttc gcgctgaccc ccgaaacgct
      121 tggcgtcacc gtgtccgccg cgctctgggg aactttggtc ggcgccctgt tcgcgggcaa
      181 gcccggcgac gccttcggta gccgcgacag tctgcgcgtg ctcgccatcc tctatttcgt
      241 cgcggggctc ggctgcgcgc tcgcgcccag ctggacggcg tttctcgtct tccgtttcat
      301 atgcgggctg gcgataggcg gctcgtcggt cctcgccccc gtctatatcg ccgagatcgc
      361 gccaccgcgg caccgcggct ttctcgtcgg cctgttccag ctcatgatcg tcaccggcat
      421 cctcgtcgcc tatctcagca acgcgacgat cgcagggctg atcggcggag agacggcgtg
      481 gcgctggaaa ctgggcgtga ccgccgcccc tgccctgctc ttcttcgtcc tgctcttcgc
      541 gattcccaac agcccgcgct ggctgctcgc caagggacgc cgcgacgaag cgggcatggc
      601 gatgacgcgc atcggtgcac cgaccgccga gctcgaccgg atcgaaacgg cgcttgaacg
      661 cgatccgccg ggcgagaggc tgtcgtggcg ccagcaccgc cggccgatgc tgctcgccat
      721 cctgatcgcg atgttcaacc agctcgcggg catcaacgcg gtcctttatt atctgaacga
      781 tattttcgcg cgcgcggggt cgctatcgcc cgaccggcaa gccgtgatga tcggcatcgc
      841 caacctcgtc ttcaccctcg ccggcatggc gctgatcgac cgcctcgggc gcaagacgct
      901 gctgctcgcg ggcgcggcgg gcatgacgct gtgcctcgcc gccgcggccg gcgtgctgtt
      961 cggcgtgctg gacaatgcac tgatgctgcc cgcactgatc gggttcatcg cctttttcgc
     1021 gaccagccag ggtgcggtca tctgggtcta tatttcggaa atcttcccga ccccggtgcg
     1081 cgcgcgcggc agcgcgctcg gcgcgagcac gcactggttg atgaacgcgc tgatcgccgg
     1141 gatctttccg gtgctcgtcg cctggtcgcc cggcgcgccc ttcgtcatct tcgccgccgc
     1201 gatggtcgtt cagttcgtcg tcgtcgcggc ggtctttccc gaaaccaaag gcgtcgatct
     1261 cgacgacatg gcgcagcgga tcgattaggt ccggtcagcg ccccgcctgc atcaggatcg
     1321 attccgaggg caggatatgg tcgaggaagg ggatgatcgc ggcgccgatc gccgaggcgt
     1381 cgtcggccgc ccccgcgcgc cgcaccggcg ccaccgccgg aagctggagc cccgtcatcc
     1441 gggcgttgag ccgctcgacc agcccatcga ccaatttgcc cgggagccgc ccgccgatca
     1501 ggatcgcggc ggggttgatc aggcagttga ccgcgatcag cggctggacg agcgcatcgg
     1561 cggcatcgtc gagccatttc aggatcaccg cctcgcgatc gggcgaactg ccgagcagcg
     1621 cgtcgggggc atcgacgtcg tgcccttcgg cttcgagccg cgcgtaaagc gccgacagcg
     1681 acaccgtgtc ctgcacattg gcgccatcgc cgcgttcggg gtcgggcatc gcccaaatct
     1741 cccccgaccg cgaatcggcg ccgcggacat agctgcggtc gatgacgagt cccccgccca
     1801 gccccgcgct gatcagcaga tagaagaagc tcggcagcgc gatgccgtcg ccgagatgca
     1861 cttcgccgag cgccgccgag gccgcgtcat tgtcggcgtg cagcggccag gggagcacct
     1921 cgccgagcag cgcgccgagg tcgatcgcgt cccacgcgtc atattccttc ggacggtgcg
     1981 gcagcgcgat gcggccaagg tcgtcgggga gcgcgatccc gacgccgagg atgcgcgccc
     2041 gatcgacgcc gccctcctcg agcaggccgg ggaggatgtc gcgcacatga tcgacgaccg
     2101 ttcccggcag cgcgaaagcg atctcgcgcg tgtggcggct gcggatcttg cccgcaagat
     2161 cgagcgtcac cagcgtgata tgatcgcggt cgatgttgag cccgatcgca aaggcgccgt
     2221 cgggatcgat gacgatccgc atcgcgggct ggccgcgctt gccctgcagg cggcccgccg
     2281 gcttgacgag gccgagatcg atcagccgct tggtgatgtt cacgatcgtc ggcggcgtga
     2341 ggccggtcac gcgcgcgagg tcgctgcgcg tcgtctcttc ggtcaggcgg atcgcctgga
     2401 gcacgatccg ctggttatag tcgcccgcgc gctcgagatt cgtgcccgac aggctgagcg
     2461 tcagccgcgc atcgcgtgcc tcggggccgc tcgtggccga ttggcggcgg tcgcgacccg
     2521 gaaggttgag gctcattgca ggactgtcct ccaagctggt tcgaatgccc ggcgcccccc
     2581 gccaaacaga ctcatgccat aaagcgtagc cgctttaata attcgatttt cgataaagcc
     2641 gagtggcggc gtcctgtcaa tcgaacgccg ccggatatca gcgcttcgac gcgatataag
     2701 cggcgacctg ttcctcgagg atgctcagcg gcagcgcgcc ttcgccgagc accgcttcgt
     2761 gaaattcgcg aatgtcgaag cgcgcgccga gttcgcgttc ggcgcgctcg cgcagttcgg
     2821 cgatcttgag ctgtccgacc atgtagctcg tcgcctgccc tggccagttg aaatagcggt
     2881 cgacctcgcg cgcgatgtcg gtgtcggaaa ccgagctgtt ggcgcggaaa taggcgatcg
     2941 cctgttcgcg cgtccaccgc ttcgaatgga tgcccgtgtc gagcaccagc cggatcgcgc
     3001 gccagacctg cagcgacaac atgccgaacc ggtcgtaggg cgtcttgtat atgcccattt
     3061 cgttcgcgag ccgctcggaa tagagccccc agccctcgat ataggcgcca tagccgccga
     3121 acttgcggaa cttcggaatg ccggccagct cctgctgccg cgcgatctgg aaatgatggc
     3181 ccggcgcgcc ctcgtgcgcg gcgatcgccg cgacctgaac cttttgcgtc tggttcatgt
     3241 cgacgagatt gacataatag atgccgggcc gcgtcccgtc ggccgatggc gggttataga
     3301 aagcggtcga cgcggtcgcc tcgcgccatt tctccaccgc gcgcacttcg agcgccgcct
     3361 tgggcagcac gctgaaatag cgcggtgccg ccgccatcgc cgcggcgatc accgcgcgcg
     3421 catcggtcag ataggcttcg cggccggcgg ccgtgttggg atatttgagc ttcggatcgg
     3481 tgcggatatg gtcgaaaaat tgttcgagcg tgcccttaaa accgacctcg cgcttgatcg
     3541 cttccatttc ctcccggatc gccgcgacct gcttcaagcc cagttcgtgg atctggtcgg
     3601 cggtaagatc ggtggtcgtc gaattcttca gccggtcggc ataataggcc gcgccgcccg
     3661 gcaggctcca cacgccgaaa ttgcccttcg actggggctc gatttcgtcg atcgcggcga
     3721 tcagcacgtc ataaccatgt ttgaacgggc cggtcagcgc cgccgtcgca tcggcgatca
     3781 gcttcgcctt gaccgcgtcg ggcgcatcga gcgcgccgac ctttttgcgg aaatcggcga
     3841 gcagcgtcga atccgccccg ccatcgaacg gcgcgccgac gaggattttc ttcgcatcgg
     3901 cgcgcgcggg cgcgaaattg accttgttgg gcacggcacc agcggcggcc tgctcgcgca
     3961 tcgtcgccgc gacctcgccc atcacgcggt cggtgtcgcg caggcgcgcg atataggcct
     4021 cggcgtcggc gacgctatcg accttgtgat tgttgatcag cagcaccgga atatcgccgg
     4081 ccgggctgcc gttggtcgtc accgggaagc ggagcttgcg gaagggaaag gacgcacggc
     4141 ggcgctcgac gtcatattcg aacaagcgat aactcacgcg cgcgctcgcg cccaattgtt
     4201 cgggccggaa gcgcgctttc atttccttga gttgctgctc gatcagcgcc tgctcgcgca
     4261 ccgccgcggc gtcggtataa tcgtcgagcc ggtcgtagtt ggtcttgagc ccaagatgcg
     4321 tctggctttc gggctggagc gcgatccgcg cgtcaaaggc gtcgtcgaga aagcggagca
     4381 gcgcttcgtc ctgcgcggcc gccggcgccg tctcggccat cgcgggggcg ggaaggacgg
     4441 caagcggcaa tagcggcatc agcatcaggg caattgcgaa acgcatgaaa attctcctgt
     4501 cgaatgcgcc acttgtaccc accgcttgcg tcggcgcaat cactacccga cggcaccggg
     4561 tcgatggcgg ggagacctcg cttcggcgat tgcagacgcg acacggcccg gctagcgcct
     4621 tgcgcatgac aatgccccgc tcccgccgcc tcgccctgct cgccgcgctt gtctcgctcg
     4681 ggctcgcgct atcccccgcg gcc
//
LOCUS       DATMSU010000021         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_180694,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000021 DATMSU010000000
VERSION     DATMSU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180694"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VN034_00510"
     CDS             <1..187
                     /locus_tag="VN034_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59106.1"
                     /translation="FGGWRGLAARGERGCQKGGGGEDETMFHASETSRRDAPAQRSSR
                     HDREPHFRLGFAAFDPI"
     gene            193..>2138
                     /locus_tag="VN034_00515"
     CDS             193..>2138
                     /locus_tag="VN034_00515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HWV59107.1"
                     /translation="MTDTPENTAPVAPANQPNTNAYGADSIKVLKGLDAVRKRPGMYI
                     GDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLITLNSDGSVSVEDNGRGIPTGIHAE
                     EGISAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGE
                     EHWMRFEHGDSVGPLKVNGPAPAGKKGTRVTFQASTETFKNVLEFDFEKLEHRYRELA
                     FLNSGVRIKLVDARHAEHVSHDLFYEGGIGAFVRYLDRNKTALLPDPIAISSERDGIG
                     IDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNGYGDKSGILKKEKVS
                     LTGEDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADRMTEWLEENPAY
                     AKAVIQKVIDAAAAREAAKKARELTRRKGAMDIASLPGKLADCQERDPTKCELFLVEG
                     DSAGGSAKQGRDRKVQAILPLKGKILNVERARFDRIISSKEVGTLIQALGTGIRDEFN
                     LEKLRYHKIVIMTDADVDGAHIRTLLLTFFYRQMPEIIEGGHLYIAQPPLYKVAKGRS
                     EVYLKDDTALENYLVDGGIDALMLETTGGARSGADLRDLIEHGRRLRALMRYVPRGHN
                     YELIEALALNGALDPELDSAGRTAA"
BASE COUNT          439 a          690 c          659 g          350 t
ORIGIN      
        1 tttcggcggg tggcgcgggc tggctgcacg cggcgagcgc ggctgtcaga agggcggcgg
       61 cggggaagac gagacgatgt ttcatgcctc tgaaactagc cgccgcgacg cccccgcgca
      121 acgctcgtcg cgacacgacc gagagccgca ttttcgcttg ggttttgctg ccttcgaccc
      181 tatataatcg ctatgacgga caccccagaa aatacggcgc cggtcgcgcc cgccaaccag
      241 cccaacacca acgcctatgg cgccgattcg atcaaggttc tcaagggcct cgacgcggtg
      301 cgcaaacgcc cgggcatgta tatcggcgac accgacgacg ggtcgggcct gcaccacatg
      361 gtgttcgagg tttcggacaa tgcgatcgac gaggcgcttg cggggcattg cgaccttgtg
      421 ctgatcacgc tcaacagcga cggatcggtc agcgtcgagg ataatggccg cggcatcccg
      481 acgggtatcc acgccgagga aggcatttcg gcggccgagg tcatcatgac ccagctccac
      541 gccggcggga agttcgagaa caccagcgac gacaatgcgt acaaggtgtc gggcggactg
      601 catggcgtcg gcgtcagcgt cgtcaacgcg ctcagcgaat ggctcgaact cacgatctgg
      661 cgcgacggcg aagagcattg gatgcgcttc gaacatggcg attcggtcgg cccgctcaag
      721 gtcaacggcc ctgcccccgc gggcaagaag ggcacgcgcg tcacctttca ggcgtcgacc
      781 gagacgttca agaatgttct cgaattcgat ttcgagaagc ttgagcatcg ctatcgcgaa
      841 ctcgccttcc tcaactccgg cgtccgcatc aagctcgtcg acgcacgcca tgccgagcat
      901 gtcagccatg atctgttcta cgaaggcggc atcggcgcgt tcgtgcgcta tctcgaccgc
      961 aacaagaccg cgctgctccc cgatccgatc gcgatcagca gcgagcgcga cggcatcggc
     1021 atcgacgtcg cgctcgagtg gaacgacagc tattacgaaa atgtcctctg cttcacgaac
     1081 aatatcccgc agcgcgacgg cggcacgcac ctcgccgcgt tccgcgccgc gctgacgcgc
     1141 acgctcaacg gctatggcga caagtcgggc atcctgaaga aggaaaaagt ctcgctgacc
     1201 ggcgaggata tgcgcgaagg gctgaccgca atcgtctcgg tcaagctgcc cgatcccaaa
     1261 ttctcgtcgc agaccaagga caagctcgtc tcgtcggagg tgcgccagcc gctcgaaagc
     1321 ctgatggccg accggatgac cgaatggctc gaggaaaatc ccgcttacgc caaggcggta
     1381 atccagaagg tcatcgacgc cgccgccgcg cgcgaagccg cgaaaaaggc gcgcgaactg
     1441 acgcggcgca agggcgcaat ggacatcgcc tcgctccccg gcaagctcgc cgactgccag
     1501 gaacgcgatc ccaccaaatg cgaactcttc ctcgtcgagg gtgactcggc aggcggatcg
     1561 gcgaagcagg gtcgtgaccg caaggttcag gcaatcctgc cgctcaaggg caagattttg
     1621 aacgtcgagc gcgcgcgttt cgaccgcatc atctcgtcga aggaagtcgg gacgttgatc
     1681 caggcgctcg ggacgggcat ccgcgacgag ttcaacctcg agaaactgcg ctatcacaag
     1741 atcgtcatca tgaccgacgc cgacgtcgac ggcgcgcata tccgcacctt gctgctcact
     1801 ttcttctatc gccagatgcc cgagatcatc gagggcggcc atctctacat cgcccagccg
     1861 ccgctctaca aagtcgcgaa ggggcggagc gaagtgtatc tgaaggatga taccgcgctc
     1921 gaaaattacc ttgtcgacgg cggtatcgat gcactgatgt tagagaccac cggcggtgcg
     1981 cggtcgggcg cggacctccg cgacctgatc gagcatggcc ggcgtctgcg cgcgctgatg
     2041 cgctatgtcc cgcgcggcca taattatgaa ctcatcgaag ccctggcact gaacggcgcg
     2101 ctcgatcccg agctcgacag cgctggccgc accgcggc
//
LOCUS       DATMSU010000022         4121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_76464, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000022 DATMSU010000000
VERSION     DATMSU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76464"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VN034_00520"
     CDS             <1..730
                     /locus_tag="VN034_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV59108.1"
                     /translation="TCNGADDCRRAVRLQIGRGADVIKFASTGGVNSRIGAGLGKQMF
                     DDEAQAIVDTAHLFGKKVAVHAHGADGIRLAIDAGADSIEHGTILDEATIAAWAKSKT
                     YYVPTLSTVNGYKERLAANPDAYEPDVLAKIKWRIEITGKSLQQLVPRGVRIAFGTDA
                     GVSKHGRNGDEFELMVQHGMTPVEAIKAATVNAADLLGLSDQIGTIAPGKSADIIAVA
                     SDPVADVRVLKKVDFVMARGEVVD"
     gene            complement(879..1379)
                     /locus_tag="VN034_00525"
     CDS             complement(879..1379)
                     /locus_tag="VN034_00525"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HWV59109.1"
                     /translation="MPPVRHASFAPHVAPDTRLLILGSLPGARSLAARQYYAHPTNQF
                     WRLLGEVMGRPLAGMLYEERLAALRDAKVGLWDVIRSAERHTSSDSLIREAEAHDLAA
                     LIAELPDLRMVAFNGGTAAAIGRKQLPPLDGIALVDLPSSSAAHTIGFDAKLKHWLQL
                     RTALVT"
     gene            1698..2069
                     /gene="rpsL"
                     /locus_tag="VN034_00530"
     CDS             1698..2069
                     /gene="rpsL"
                     /locus_tag="VN034_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWV59110.1"
                     /translation="MPTINQLVRKGRTLQKVKSKVPAMEANPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNAREVITYIPGEGHNLQEHSVVLIRGGRVRDLPGVRYHVLRGVL
                     DTQGVKDRKQSRSKYGAKRPK"
     gene            2143..2613
                     /gene="rpsG"
                     /locus_tag="VN034_00535"
     CDS             2143..2613
                     /gene="rpsG"
                     /locus_tag="VN034_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWV59111.1"
                     /translation="MARRRRPERREILPDPRFGDVVLSKFMNSVMLDGKKSVAESIVY
                     GALESVEARAKKEPIGVFHEALANIRPNIEVRSRRVGGATYQVPVEVRPDRAQALAIR
                     WLITAARNRSETTMAARLSGELLDASNNRGNAVKKREDTHRMAEANRAFSHYRW"
     gene            2724..>4121
                     /gene="fusA"
                     /locus_tag="VN034_00540"
     CDS             2724..>4121
                     /gene="fusA"
                     /locus_tag="VN034_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006960295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWV59112.1"
                     /translation="MARSHPLERYRNFGIMAHIDAGKTTTTERILYYTGKSYKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCLWKAEEGQGPEHRLNIIDTPGHVDFTIEVERS
                     LRVLDGAITAFDGVAGVEPQSETVWRQADKYKVPRMCFVNKLDRTGADFYYCVQTIID
                     RLGATPAVLYLPIGAEGDFKGLVDLVNERAIIWKDESLGAEFFYEEIPADLADKAAEY
                     REKLVELAVEQDDDAMEAYLEGTVPDVAQLKALIRKGTLAQAFVPVLCGSAFKNKGVQ
                     TLLDAVVDYLPSPLDIPDVQGINPDTDQPDSRETSDAAPLSLLAFKIMNDPFVGSLTF
                     ARIYSGTLNKGTYLNSVKDKKEKVGRMLLMHANSREDIEEAYAGDIVALAGLKETTTG
                     DTLCATNAPIILERMEFPEPVIELSVEPKTKADQERMGIALNRLAAEDPSFRVSTDHE
                     SGQTIIKGMGELHLDI"
BASE COUNT          803 a         1357 c         1283 g          678 t
ORIGIN      
        1 cacgtgcaac ggcgccgacg actgccgccg cgcggtgcgg ctccagatcg ggcgcggcgc
       61 cgacgtgatc aaattcgcct cgaccggcgg cgtcaacagc cgcatcggcg ccggcctcgg
      121 caagcaaatg ttcgacgacg aggcgcaggc gatcgtcgac accgcgcatc tgttcggcaa
      181 gaaggtcgcg gtccacgcgc acggcgccga cggcatccgt ctcgcgatcg atgccggcgc
      241 cgactcgatc gagcatggca cgatcctcga cgaggcgacg atcgcggcct gggcgaaatc
      301 gaagacttat tatgtgccga ccctctcgac agtgaacggc tacaaggaac ggcttgcggc
      361 gaatcccgac gcctatgaac ctgacgtgct cgccaagatc aaatggcgca tcgagatcac
      421 cggcaagagc ctccagcaac tcgtcccgcg cggcgtgcgc atcgccttcg gcaccgacgc
      481 cggtgtgtcg aagcacggcc gtaacggcga cgagttcgaa ctgatggtcc agcacgggat
      541 gaccccggtc gaggcgatca aggccgcgac ggtgaacgcc gccgacctgc tcggcctgtc
      601 ggaccagatc ggcacgatcg cgccgggcaa gagcgccgac atcatcgcgg tcgcgagcga
      661 cccggtcgcc gacgtgcgcg tgctcaagaa ggtcgatttc gtgatggcgc gcggcgaggt
      721 ggtcgattga tccagccaga tcctccccat cgcgaagcgt tggggagggg aaccgtccgc
      781 gaagcgggtg gtggaggggt ttaaacgccg gcgccatgac ccctccgtca gcgctttgcg
      841 ctgccacctc cccttcgctg cgcgatgggg aggatcgatt aggttactag cgccgtgcgc
      901 aattgcagcc aatgtttcag cttcgcatcg aagccgatcg tgtgcgcggc gctgctcgac
      961 ggcagatcga cgagagcgat gccatcgagc ggcggcagct gttttcgccc gatcgccgcc
     1021 gccgtgccac cgttgaaggc gaccatgcgc aaatcgggca gctcggcgat cagtgccgcg
     1081 agatcgtgcg cttcggcttc gcggatcagc gagtcgctgc tcgtgtgccg ctcggcgctg
     1141 cggatcacgt cccacagccc gacctttgcg tctcggagcg cggccaacct ctcttcatag
     1201 agcatccccg ccagcggccg gcccatcacc tcccccagca ggcgccagaa ctggttggtc
     1261 gggtgtgcat aatattgccg cgcggcgagc gagcgggcgc cgggcaggct tcccaatatc
     1321 agcagccgcg tgtcgggcgc gacatgcggg gcgaagctgg cgtggcggac ggggggcatg
     1381 gacccgcgat agcccgctcg cgctcgcgca acaatattgc ccgtccctcg gcccgccctc
     1441 ttgaccatcg ggggaatcgg gtctaggggc gcgctcgacc gaaaagggcg gtttgccgcc
     1501 tctttcagtt gcaacagcgc ttgaacattg ctggcgcgga tggagcctcc ggaagatctt
     1561 gtaggtctgc cggggtcttt tgctgttatc aggtcggccg ttttccgcca ttttcggtgg
     1621 gttagggtcg gagcttcatc caccgcggta cagtaaccgt tcgctttgat gggcggacac
     1681 aaaaaggtga agcattcatg cccacgatca accagctggt ccgcaagggc cggactctcc
     1741 agaaggtgaa gtccaaggtc ccggcgatgg aagcaaaccc gcaaaagcgc ggcgtttgca
     1801 ctcgtgtcta tacgacgacc ccgaaaaagc cgaactcggc gctccgcaag gttgcaaagg
     1861 tccgcctgac caatgcccgc gaagtcatca cttacatccc cggcgaaggc cacaacctcc
     1921 aggaacacag cgtcgtgctg atccgcggcg gccgtgtgcg cgaccttccc ggtgtgcgtt
     1981 accacgtcct gcgcggcgtg ctcgatacgc agggtgtgaa ggaccgcaag cagagccgtt
     2041 cgaaatacgg cgcgaagcgt ccgaagtaag gaccgctttt tcggctattt gatcatcccg
     2101 ggacgccaac gcgccgggat gctgttgtaa aaggaattac ctatggctcg tcgtcgtcgt
     2161 cctgaacgcc gcgaaatcct gcccgatccc cgtttcgggg atgtcgtgct gtcgaaattc
     2221 atgaacagcg tcatgctgga cgggaagaag tccgtcgccg aaagcatcgt ttacggtgcg
     2281 ctcgagtcgg tcgaagcccg cgccaagaag gaaccgatcg gcgtgttcca cgaagcgctg
     2341 gcgaacatcc gcccgaacat cgaagtccgc agccgccgcg tcggcggtgc gacctatcag
     2401 gttccggtcg aagtccgccc cgaccgcgca caggcgctcg cgatccgctg gctgatcacc
     2461 gccgcgcgca accgcagcga gaccacgatg gccgcgcgtc tgtcgggcga actgctcgac
     2521 gcgtcgaaca accgcggcaa cgcggtcaag aagcgcgaag acacgcaccg tatggccgaa
     2581 gccaaccgcg ccttctcgca ctaccgctgg taagcgcgcg cggggcgccc ccacctctct
     2641 tgggccgcgt ctcgcaatcg taacaatcct tcctatatcg gaggcggccc gaaaggccgc
     2701 cccccaaatc caaggaaaag accatggcac gcagccatcc gctcgaacgc tatcgcaatt
     2761 tcggtatcat ggcgcacatc gacgccggca agaccacgac gaccgagcgc attctctatt
     2821 acaccggcaa gtcctacaag atcggcgaag tccatgacgg cgccgcgacg atggactgga
     2881 tggagcagga gcaggagcgc ggcatcacga tcacgtcggc tgcgaccact tgcttgtgga
     2941 aggccgaaga aggtcagggc cccgagcatc gcctgaacat catcgacacc cccggccacg
     3001 tcgacttcac gatcgaagtc gagcgcagct tgcgcgtgct cgacggcgcg atcaccgcgt
     3061 tcgacggcgt tgccggcgtc gagccgcagt cggaaaccgt gtggcgtcag gcggacaagt
     3121 acaaggttcc gcggatgtgc ttcgtcaaca agctcgaccg caccggtgcc gatttctact
     3181 attgcgtcca gacgatcatc gatcgccttg gtgcgacccc ggccgtcctc tatctgccga
     3241 tcggcgccga aggcgacttc aagggtctcg tcgacctcgt caacgagcgc gcgatcatct
     3301 ggaaggacga aagcctcggc gccgaattct tctatgagga aatccccgcc gacctcgctg
     3361 acaaggccgc cgaatatcgc gaaaagctcg tcgagctcgc cgtcgaacag gacgacgacg
     3421 cgatggaagc ctatctcgaa ggcaccgtcc ccgacgtcgc ccagctcaag gcgctgatcc
     3481 gcaagggcac gctggcacag gcgttcgtcc cggttctttg cggctcggcg ttcaagaaca
     3541 agggtgtgca gactttgctc gacgcggtcg tcgattacct gccttcgccg ctcgacattc
     3601 ccgacgttca gggcatcaat ccggacaccg accagcccga ctcgcgcgag acgtcggacg
     3661 cggcgccgct gtcgctgctc gccttcaaga tcatgaacga cccgttcgtc ggttcgctga
     3721 ccttcgcccg catctattcg gggaccctga acaagggcac ctatctgaac tcggtgaagg
     3781 acaagaagga aaaggttggc cgcatgctgc tgatgcatgc gaacagccgc gaagacatcg
     3841 aagaagccta tgcgggcgac atcgtcgcgc ttgccggcct caaggaaacc accaccgggg
     3901 acacgctctg cgccaccaac gcgccgatca tcctcgagcg gatggaattc cccgagccgg
     3961 tcatcgagct gtcggtggaa ccgaagacca aggccgacca ggaacgcatg ggcatcgcgc
     4021 tcaaccgcct ggccgccgag gatccctcgt tccgcgtgtc gaccgaccat gaatcgggcc
     4081 agacgatcat caagggcatg ggcgagcttc acctcgacat c
//
LOCUS       DATMSU010000023         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_10132, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000023 DATMSU010000000
VERSION     DATMSU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10132"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1560)
                     /locus_tag="VN034_00545"
     CDS             complement(4..1560)
                     /locus_tag="VN034_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWV59113.1"
                     /translation="MLKQLSAQDAQFLYTQTANNLTHIMGVYIYDPATAPGGFVRFKD
                     IIRHVESRVDTSPLFKRRLHRLPFDMDHPYWVEDEHFDIEAHMSHARLPEPGDWRQFC
                     IAVARWFSKPMDMNRPLWDIYIIEGLDRIPGIPKGSFAMLHRVHHAAVDGASGAHAFI
                     AMSDIDAKGTPAIAEPPPVEELGKAPSSAETLSRAWSASMQSPVKFMNALMKMSPAIV
                     ASARKSIAEGGMTAGVPETRFNVPVGPHKMFDATTVALADVAEIRRKVPGATVNDVVL
                     TTVGGALRKYLAKHKELPKESLVAVAPINLRGKGGKASTPGNQVSAMSVPVRTDIAGP
                     LERLAAIRDYTVEAKEAKAGVSAGIMTDLSQHIPGATMAAVARLVTSERFAVRGTNLF
                     ISNVPGAQVPLYLAGAQLVQQHGMAPLANNMGLFVATPSYNGRIAFSIVCERSIMPDI
                     AFFRECIDESFVDLMAATPKPGKVKAATATPKAAPKPATKTKVKAGPAAKAKTKGRGK
                     GKSSSSSSRT"
     gene            complement(1677..>3536)
                     /locus_tag="VN034_00550"
     CDS             complement(1677..>3536)
                     /locus_tag="VN034_00550"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HWV59114.1"
                     /translation="VARQAALALDVAERGIRGTRYKVPRFVADGLRTSPKFRAALAEL
                     AERTGRPVADLFREARPLMKEVIARPSALFLDLRARLDRMMFGGYAPEMEVDAVELLK
                     LRGILREHPTCILFTHKTYIDGATPSRLAYENDMPMLHSFGGANLDFAVMGEFFRRSG
                     MIFLRRSFQDQPVYKLVLRHYIAWLLAKRFPLSWAFEGTRSRLGKLMPPKYGLMKYVL
                     DAAHATGTRGVHFVPFVTSFDLIRDVEEYAAEQTGRNKKPESLSWFLGYMKSLKEPSG
                     RIRLDIGEPVVIETAPGPDDRRALEKIAFAVAVEANRVTPLTVTSVMCLILLGMAPRG
                     ATSAELLGAIGAVTDWARARDIRLSKELESGDDAALSATVDTLVASGLLTRYEAGSEN
                     VYSIDPARHPMASYYRNIIAHHFLGRAMIELALFELRDADKGDATAAFWAKIDRLRDL
                     FKFEFFYPPRDEHRAAIEAELTRIDPVWDRRLASGDRGIAQLLRRCQPVVGHAILLPF
                     AEAYSVVAEQLARARPGDAVDEQALLDAALVEGRQAYLLRRISSEAAIGKLLFANGLS
                     LMRHMGLAEEATPVNLAARRALLMELRGLANVMETMRLSTVALADRLPGLPNV"
BASE COUNT          609 a         1157 c         1147 g          623 t
ORIGIN      
        1 ggatcaagtc cgggatgacg aagaggagga tttccccttg ccccgtcctt tcgttttggc
       61 ctttgcagcg ggtcccgcct tgaccttggt cttcgtggcc ggcttcggcg ccgctttcgg
      121 tgttgccgta gcggctttta ccttcccggg tttcggcgtc gccgccatca aatcgacgaa
      181 actttcgtcg atgcactcgc gaaagaaagc gatgtcgggc atgatcgacc gctcgcaaac
      241 gatcgaaaag gcgatgcgcc cgttatagct cggcgtcgcg acgaacagcc ccatattgtt
      301 cgcgagcggc gccatgccat gctgctgcac gagctgcgcg cccgcgagat agagcggcac
      361 ctgcgccccc ggcacattcg agatgaagag attggtgccg cgcaccgcga accgctcgct
      421 ggtgacgagc cgcgcgaccg ccgccatcgt cgcgcccgga atatgctgcg acaggtcggt
      481 catgatcccc gcgctcaccc ccgccttcgc ttccttcgcc tcgaccgtat agtcgcggat
      541 cgccgctagc cgttcgagcg gccccgcgat gtcggtgcgg accggcacgc tcatcgccga
      601 cacctgattg cctggggtcg acgcctttcc gcccttgccg cgcaggttga tcggcgcgac
      661 cgcgacgagg ctctccttgg gcagctcctt gtgctttgcg agatatttgc gcagcgcgcc
      721 gccgacggtg gtcagcacga cgtcgttgac cgtcgcgccg ggcaccttcc gccggatttc
      781 ggcgacgtca gcgagcgcga ccgtggtcgc gtcgaacatc ttgtgcgggc cgacgggcac
      841 gttgaagcgg gtttcgggga cgccggcggt catgccgccc tcggcgatcg atttgcgtgc
      901 cgacgcgacg atcgcgggcg acatcttcat cagcgcgttc atgaacttca ccggcgactg
      961 catcgacgcc gaccaggcgc gcgagagggt ttcggcgctc gacggcgcct tgcccaactc
     1021 ctcgaccggc ggcggttcgg caattgccgg cgtgcccttc gcatcgatat cgctcatcgc
     1081 gatgaaagca tgcgcgcccg aggcgccatc gaccgcggcg tggtggacgc ggtggagcat
     1141 cgcgaagctg cccttgggaa tgcccgggat gcggtcgagc ccctcgataa tgtagatgtc
     1201 ccagagcggc cggttcatgt ccatcggttt cgaaaaccag cgcgcgaccg cgatgcagaa
     1261 ttgccgccag tcgccgggtt cgggcagccg cgcatggctc atatgcgcct cgatgtcgaa
     1321 atgctcgtcc tcgacccaat agggatgatc catgtcgaac ggcaggcggt ggagtcggcg
     1381 cttgaacaag ggcgaggtat cgacgcggct ctcgacgtgg cggatgatgt ccttgaaacg
     1441 cacgaagccg cccggcgcgg tcgcgggatc gtagatatag actcccatga tgtgcgtcag
     1501 attgttcgcg gtctgcgtgt agaggaactg ggcgtcctgc gcgctgagct gtttgagcat
     1561 cgaaaagtcc tccaacatct aacctgccgt catcccggcg caggccggga tctcaccctc
     1621 tcgaaatgtc gcaccggcga gatcccggcc ttcgccggga tgacgaaaac gggagctcag
     1681 acattgggga gccccggcaa ccgatccgcc agcgccaccg ttgaaagccg catcgtttcc
     1741 atgacgttgg cgaggccgcg cagttccatc agcagcgcgc ggcgcgcggc tagattgacc
     1801 ggcgtcgctt cctcggcgag ccccatatgg cgcatcagcg acaggccgtt ggcaaagagc
     1861 aatttgccga tcgccgcctc gctgctgatc cggcgcagca gataggcctg tcgtccctcg
     1921 acgagcgccg catcgagcag cgcctgttca tcgaccgcat cgccgggcct cgcgcgcgcc
     1981 agctgttcgg cgacgaccga ataagcctcg gcgaagggca gcaatatcgc atggccgacg
     2041 acgggctgac agcggcggag tagctgggcg ataccgcggt cgccgctcgc gagacgacgg
     2101 tcccacaccg ggtcgatgcg cgtcagctcg gcctcgatcg ccgcgcgatg ctcatcgcgc
     2161 ggcggataga agaattcgaa tttgaacagg tcgcgcagcc gatcgatctt cgcccagaag
     2221 gcggcggtcg catcgccctt gtcggcgtcg cgcaactcga acagcgcgag ttcgatcatc
     2281 gcgcgaccca agaaatgatg cgcgatgatg ttgcgataat agctcgccat cggatgcctg
     2341 gcggggtcga tcgaatagac attctcgctg cccgcttcat agcgcgtcag cagcccgctc
     2401 gcgacgagcg tgtcaaccgt cgccgacagc gcggcatcat cgccgctttc gagctccttg
     2461 ctgagccgga tatcgcgcgc ccgcgcccag tcggtgaccg caccgatcgc gccgagcagt
     2521 tcggccgagg tcgcgccgcg cggcgccatg ccgagcaaga tcaggcacat caccgaggtg
     2581 acggtgagcg gagtgacgcg gttcgcctcg accgcgaccg cgaaggcgat cttctcgagt
     2641 gcacgcctgt cgtccggccc cggcgcggtt tcgatgacga ccggctcgcc gatgtcgagg
     2701 cggatacgcc ccgatggctc cttgaggctc ttcatatagc cgagaaacca cgacaggctt
     2761 tcgggcttct tgttccgccc cgtctgctcg gcggcatatt cctcgacatc gcggatcagg
     2821 tcgaagctgg tgacgaaggg cacgaaatgg acgccgcgcg ttccggtcgc gtgcgccgcg
     2881 tcgaggacat atttcatcag gccatatttg ggcggcatca gcttgccgag ccgcgagcgc
     2941 gtcccctcga acgcccagct caagggaaaa cgcttggcga gcagccaggc gatatagtgg
     3001 cgcagcacga gcttgtagac cggctggtcc tgaaagctgc ggcggaggaa gatcatcccc
     3061 gagcggcgga agaattcgcc catcaccgcg aagtcgaggt tggcgccgcc gaagctgtgc
     3121 agcatcggca tgtcattctc ataggcgagg cggctcggcg tcgcgccgtc gatataggtt
     3181 ttgtgcgtga agaggatgca ggtggggtgt tcgcgcaaaa ttccgcgcaa tttcaacaac
     3241 tcaacggcgt ccacctccat ctcgggcgca tagccgccga acatcatgcg atcgagacgt
     3301 gcgcgaagat cgagaaagag cgccgaaggc cgcgcgatga cctccttcat cagcggccgc
     3361 gcctcgcgga acaggtcggc gaccggacgc cccgtcctct ccgccagctc ggcgagcgcg
     3421 gcgcggaatt tggggctggt gcgcaaacca tcggcgacga agcgcggcac cttgtatctt
     3481 gttccccgga taccgcgttc ggcgacgtcg agtgcgagcg ccgcctgtcg cgcgac
//
LOCUS       DATMSU010000024         4068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_57407, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000024 DATMSU010000000
VERSION     DATMSU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57407"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VN034_00555"
     CDS             complement(<1..429)
                     /locus_tag="VN034_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59115.1"
                     /translation="MAKSLKRPLSLALGALAATTLGGCYYGDVYGASYASGGDCAARY
                     GDAYYDYDGYAYDDGYGYDCYDASDYGSGFVNIGFGGGWYDDYYYPGYGMWMFDNYRN
                     RYPLRDRYLNYWGGRRAWWKHHGGRKDWKPGRPGWHRGDRD"
     gene            601..2007
                     /locus_tag="VN034_00560"
     CDS             601..2007
                     /locus_tag="VN034_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV59116.1"
                     /translation="MNAPTNIQPANSQWSETRFGPDTRHWLPRNPDSAIDHIPGEDGM
                     PVIGNTLEQLRDYPAFTRRMVAKYGRVYRNNSFGGRSVALHGPEANELIMFDRDKIFS
                     SEQGWGPVLNLLFPRGLMLMDFEKHRADRKVLSVAFKPEPMRHYADSLNEGIKDRIGQ
                     WSGKTFKFYPAIKELTLDLAATSFLGIPWGPEADRVNKAFVDMVQASIGVVRVPLPFT
                     AMGRGAKGRAYLVDYFGKMVPQRREGSGEDMFSQICRTRDDDGEYMSVEAIVDHMNFL
                     MMAAHDTITSSITTLVWQLARNPEWQDRLREEMLSVAPAGEGVGHNSLGQLEMTEWAF
                     KEALRLVPPVPSFPRRALKDFEYGGYRIPAGTSVGVSPAYTHMMEEHWPEPAKFDPMR
                     FSPEVARERHKYAWVPFGGGAHMCLGLHFAYMQAKIFFHHVITTHRITVAEDYAPEWQ
                     VLPIPRPKDGLTVSFVPL"
     gene            complement(2304..3002)
                     /gene="phoB"
                     /locus_tag="VN034_00565"
     CDS             complement(2304..3002)
                     /gene="phoB"
                     /locus_tag="VN034_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HWV59117.1"
                     /translation="MPQPDLLLIEDDEAIAELIVWHFAREGFSVRQTPDGEQALILVE
                     ERVPDIVLLDWMIESLPGIEVCRRLRRNPKSANVPIIMLTARGEEEDRIRGLETGADD
                     YVTKPFSPRELVARVSAVLRRLRPALAGEMLTYADIELDSVAHKVVRGGQIVAMGPTE
                     FRLLRHFMEHPGRVFSRGQLLDSVWGQDSDIELRTVDVHIRRLRKAINLPGTSDIIRT
                     VRSAGYALDAGKSI"
     gene            complement(3002..3721)
                     /gene="phoU"
                     /locus_tag="VN034_00570"
     CDS             complement(3002..3721)
                     /gene="phoU"
                     /locus_tag="VN034_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWV59118.1"
                     /translation="MALTNDHTVKAFDEDLNRLRGLISEMGGRAEQALLQAMTALSKG
                     DLDLAAQVVRDDKKIDALEAEVEQLAVQTIALRAPMADDLREMIAALKIVSVVERIGD
                     YAKNIAKRVALMDQTRSIEAIPLLTSMSSIVVELIHDALDSFAARDAELAVRVTVRDK
                     NVDDFYNSIFRTLVTFMMENPKYISESAHLLFVAKNLERMGDHATNIAEMVHYVVTGE
                     RMEERERGEQPEDGAAEQEQG"
     gene            complement(3747..>4068)
                     /locus_tag="VN034_00575"
     CDS             complement(3747..>4068)
                     /locus_tag="VN034_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV59119.1"
                     /translation="TALSGGQQQRLCIARAIAVDPEVILMDEPCSALDPIATAKIEEL
                     IHELRGKYAIVIVTHNMQQAARVSQRTAFFHLGTLVEYGKTTDIFTNPRQERTKDYIT
                     GRYG"
BASE COUNT          731 a         1279 c         1299 g          759 t
ORIGIN      
        1 gtcgcggtcg ccgcgatgcc agccggggcg gccgggcttc cagtccttgc gtccgccatg
       61 atgtttccac caggcgcggc gcccgcccca atagttgaga tagcgatcgc gcagcggata
      121 gcggttgcgg taattgtcga acatccacat gccgtagccg ggataataat aatcgtcgta
      181 ccagccgccg ccgaaaccga tgttgacgaa accgctgcca taatccgacg cgtcgtagca
      241 gtcgtagcca tagccgtcgt cataggcata gccgtcatag tcgtaatagg cgtcgccata
      301 gcgtgcggcg caatcgccgc ccgatgcgta actcgcgcca tagacgtcgc cataatagca
      361 gccgccgagc gtcgtcgcgg caagcgcgcc gagcgcaagg gaaaggggac gcttcaaact
      421 cttcgccatg ggctttgtcc tttagggtcc aacggtcacg gcccctcact cgatcagcaa
      481 attgcacgag tcgagttgaa tttgcggtga atggtgttcc gcgccccgaa aggccttact
      541 tgcatcgatg tcagtagttg gttacgcatc gcgagagaca agatggacga ggatcgaccg
      601 atgaacgcgc cgacgaatat ccagcctgcg aacagccaat ggtccgaaac gcgcttcggc
      661 cccgacacgc ggcactggct gccgcgcaat cccgacagtg cgatcgacca tattccgggc
      721 gaggacggca tgcccgttat cggcaacacg ctcgaacagc ttcgcgacta tcccgccttc
      781 acccgccgca tggtcgccaa atatggccgc gtctatcgca acaacagctt cggcgggcgc
      841 agcgtcgcgc tccacgggcc cgaagcgaac gaactgatca tgttcgaccg cgacaaaatc
      901 ttctcgtccg aacagggctg gggtcccgtg ctcaacctgc tcttcccgcg cgggctgatg
      961 ctgatggact tcgaaaagca ccgcgccgac cggaaggtgc tgtcggtcgc cttcaagccc
     1021 gaaccgatgc gtcattatgc cgattcgctg aacgagggga tcaaggaccg catcggccag
     1081 tggagcggaa agaccttcaa attctatccg gcgatcaagg aactgacgct cgaccttgcc
     1141 gcgacgagct ttctcggcat cccctgggga ccggaggcgg acagggtcaa caaggccttc
     1201 gtcgacatgg tgcaggcgtc gatcggcgtc gtgcgcgtgc ccttgccctt caccgcgatg
     1261 ggccgcggcg cgaaggggcg cgcctatctg gtcgactatt tcggcaagat ggtgccgcag
     1321 cggcgcgagg gcagcggcga ggatatgttc agccagatct gtcgcacgcg cgatgatgac
     1381 ggcgaatata tgtcggtcga ggcgatcgtc gaccatatga acttcctgat gatggcggcg
     1441 cacgacacga tcaccagctc gatcaccacg ctcgtctggc agctcgcgcg caaccccgaa
     1501 tggcaggacc ggctgcgcga ggaaatgctg agcgtcgcgc cggcgggcga gggggtcggg
     1561 cacaacagcc tcggccagct cgaaatgacc gaatgggcgt tcaaggaagc cctccgcctc
     1621 gtgccgccgg tgccgagctt cccgcgccgg gcgctcaagg atttcgaata tggtggctac
     1681 cgcatccccg ccggcacttc ggtcggcgtc agcccggcct atacgcatat gatggaggag
     1741 cattggccgg agcccgcgaa gttcgacccg atgcgcttct cgcccgaagt cgcgcgcgag
     1801 cggcacaaat atgcgtgggt gcccttcggt ggcggcgcgc atatgtgcct cggcctgcac
     1861 ttcgcgtata tgcaggcgaa aatcttcttc caccacgtca tcacgacgca ccggatcacc
     1921 gtcgcggaag actatgcgcc cgaatggcag gtactcccga tcccgcggcc caaggatggg
     1981 ctgacggtga gtttcgtgcc gctgtaacgc agtcggcgtc tgttttgggg tggggagctg
     2041 ccactgcttt ctccagcttc gtcaccccgg gcttgacccg gggcctgcct tgccttctcg
     2101 ccatcgtcag gaagaaaagg cgggtcccgg gtcaagcccg ggatgacgat aacgagaatg
     2161 tccgcaatcg gtcgttagcg accatttccc ggtttcgtca tccccgcgaa agcggggacc
     2221 cagagcgtcc gtcggctggc ccatcactgg gttcccgctt tcgcgggaat gacgaataga
     2281 gagagtatgg cagctgtgga ccgtcagatg ctcttcccgg catcgagcgc atagcccgcc
     2341 gatcgcaccg tccggatgat gtcgctcgtc cccggcaggt tgatcgcctt gcgcagccgg
     2401 cggatatgga cgtcgacggt gcgcagttcg atgtcgctgt cctggcccca gacgctgtcg
     2461 agcaactgcc cgcgtgagaa gacgcggccc ggatgctcca tgaaatggcg gagcaggcgg
     2521 aattcggtcg gtcccatcgc gacgatctgg ccaccccgga cgactttgtg cgcgaccgag
     2581 tcgagctcga tgtcggcata ggtgagcatt tcaccggcga gcgcggggcg caggcggcgg
     2641 agcacggcgg atacgcgcgc gaccagctcg cgcgggctga acggtttggt gacataatcg
     2701 tcggcgccgg tttcgaggcc gcggatgcgg tcctcttcct cgccgcgcgc ggtgagcatg
     2761 atgatcggga cgttcgccga cttggggttg cggcgcaggc gccggcagac ctcgatcccc
     2821 ggcaggctct cgatcatcca gtcgagcagg acgatatcgg ggacgcgttc ctcgacgagg
     2881 atcagcgcct gttcgccgtc gggggtctgg cggaccgaaa agccctcgcg cgcgaaatgc
     2941 cagacgatga gctcggcgat cgcctcgtca tcctcgatca gcagaaggtc gggctgcggc
     3001 atcagccttg ctcctgctcc gcggcgccat cttcgggctg ttcgccgcgc tcgcgttctt
     3061 ccatccgctc gccggtcacg acataatgga ccatctcggc gatgttggtc gcatggtcgc
     3121 ccatgcgctc gaggttcttg gcgacgaaca gcaaatgcgc gctttccgag atatatttcg
     3181 gattttccat catgaaagtg acgagcgtgc gaaagatgct gttgtagaaa tcgtcgacat
     3241 tcttgtcgcg gacggtcacc cgcaccgcga gttcggcgtc gcgcgccgcg aagctgtcga
     3301 gcgcgtcgtg gatcagctcg acgacgatcg acgacatcga ggtgaggagc gggatcgcct
     3361 cgatcgaccg cgtctggtcc atcagtgcga cgcgcttggc gatattcttc gcataatcgc
     3421 cgatacgctc gacgaccgag acgatcttga gcgccgcgat catctcgcgg agatcgtcgg
     3481 ccatcggcgc gcggagcgcg atcgtctgca ccgcaagctg ctcaacttcg gcctcgagcg
     3541 cgtcgatctt cttgtcgtcg cgcacgacct gcgccgcgag gtcgaggtcg cccttgctga
     3601 gcgcggtcat cgcctggagc agcgcctgtt cggcgcgccc gcccatctcg ctgatcagcc
     3661 cgcgcaggcg gttgaggtcc tcgtcgaagg ctttgaccgt atgatcattc gttagtgcca
     3721 tcgttttcgt cctaatgcgc tacggatcaa ccgtagcggc cggtgatata gtccttggtc
     3781 cgctcttggc gcgggttggt gaagatgtcg gtggtcttgc cgtactcgac caaagtcccg
     3841 aggtggaaaa aggcggtgcg ctgcgacacg cgggccgcct gctgcatatt gtgcgtgacg
     3901 atcacgatcg catatttgcc gcgcagttcg tggatcagct cctcgatctt cgcggtcgcg
     3961 atcgggtcga gcgccgagca gggctcgtcc atcaaaatga cttcgggatc gaccgcgatc
     4021 gcgcgcgcga tgcacaggcg ctgctgctgg ccgcccgaca aggcagtg
//
LOCUS       DATMSU010000025         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_133322,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000025 DATMSU010000000
VERSION     DATMSU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133322"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VN034_00580"
     CDS             <1..939
                     /locus_tag="VN034_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59120.1"
                     /translation="APPPSEPPEQRVAAVPEGQGVLTRAGELFFEPSFEYTRSSTNRL
                     VFRGIELIPGIQIGLIEATDADRDTLVGTASLRYGITDRLEAEVRLPYLYRNDRIEVV
                     QQRDEGIVRQIALREHGIGDAEFSLRYQFNRPVGQKPIFVGTLRVKSDTGKGPFEVGY
                     DEFGVATGLATGSGFWAVQPGLNFLMPSDPAVIYGGAAYLYHIPRDVNELVGDALIGR
                     VDPGDAVSANIGFGFALNPRFSFSLGYRHNYIFPTKTEIGDTNQKSNYIHVGSLNFGM
                     SYRLTQRDVLNLGFEIGVTEDAPDVSITLRMPFGGK"
     gene            complement(1037..1603)
                     /locus_tag="VN034_00585"
     CDS             complement(1037..1603)
                     /locus_tag="VN034_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59121.1"
                     /translation="MSPRILLLALSPVLLAASEPEDADPLAGAQTVSDEVLAAQRGGF
                     AFQGMVIAFGADIRSFVNDELALRTIVSWTPEGQTIERFVSPSLTAVDAAQVQGGILT
                     SGGISMRVGDEKVFLANNGQTALIQSTDAIQNVLINTASNVSLNQQVDAVLDISGYEG
                     FRDALATTRLSDSIGAVMGAQTVGALGN"
     gene            complement(1612..2337)
                     /locus_tag="VN034_00590"
     CDS             complement(1612..2337)
                     /locus_tag="VN034_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HWV59122.1"
                     /translation="MRALIAGALCALAAAPAAAEVRLTGPETGGTYQLQVMTWWDIPF
                     RSVIRQRYDFSCGSAALATLLTYHYGAPTSETMPFRAMWEKGDREAIRKVGFSMLDMK
                     TYLASRGFRAEGFRLTVDQLKQVKRPTIVLMDLKGFKHFVVIKGVRGDRVLTGDSVLG
                     LNEYSLEDFEKHWNGIALAIVEGGKKRPLFNLAGDWGPWSQAPLEKDGSLHVSAGDLT
                     THLPPQYQLTPQILLDVRVGTVK"
     gene            complement(2354..2761)
                     /locus_tag="VN034_00595"
     CDS             complement(2354..2761)
                     /locus_tag="VN034_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59123.1"
                     /translation="MKPPIVPVLLIMAGLSVPVSAQLPPSDVVVPVAMESIPAETLAA
                     PAIPARPERKSPMLNDGELDSQRGGQSLVITNQTMESLVAGNTLNGDYTAGSVALSDF
                     ALSNFNGVGNLLINTGAQVSAQSGMNLTINLGD"
     gene            complement(2840..3364)
                     /locus_tag="VN034_00600"
     CDS             complement(2840..3364)
                     /locus_tag="VN034_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59124.1"
                     /translation="MIKKVMLAVSTLALAVTAAPAVAQGQDQDNDNTTTGASGNNSDN
                     DTVVVNDLIDLFVQDNAADTFSDDDQDNDGNGSFNGNSLVVATQTLTATNTNQNLDEV
                     VDLDGEDGTETAVGYNSGNNSVRGSAFAAYAGILNAAWNTGINANTQAATNIAASGTV
                     SFGDSAPAGGGGGD"
BASE COUNT          619 a         1142 c         1236 g          678 t
ORIGIN      
        1 gcgccgccgc cgagcgagcc gcccgagcag cgcgttgcgg cggttcccga ggggcagggc
       61 gtgctgaccc gcgccggcga gctcttcttc gaaccttcgt tcgaatatac gcgctcgtcg
      121 acgaaccgcc tcgtgttccg cgggatcgag ctgatccccg gcatccagat cggcctgatc
      181 gaggcgaccg acgccgatcg cgacacgctc gtcggcaccg catcgctgcg ttacgggatc
      241 accgatcgcc tcgaagccga agtgcggctg ccctatctgt atcgcaacga ccggatcgag
      301 gtcgtccagc agcgcgacga aggcatcgtc cggcagatcg cgctgcgcga acacgggatc
      361 ggcgacgccg aattctcgct ccgttaccag ttcaaccggc cggtcgggca aaagccgatt
      421 ttcgtcggaa cgctgcgcgt caaatcggac accggcaagg ggccgttcga ggtcggctat
      481 gacgaattcg gggtcgcgac ggggctcgcc accggatcgg gattctgggc ggtgcagccg
      541 gggctcaact tcctgatgcc gtcggatccc gcggtgatct atggcggcgc tgcctatctc
      601 tatcacatcc cgcgcgacgt gaacgaattg gtcggcgacg cgctgatcgg gcgcgtcgat
      661 ccgggcgacg ccgtgtcggc caacatcggt ttcggctttg cgctcaaccc gcgcttttcc
      721 ttctcgctcg gctatcgcca caattatatc ttcccgacga agaccgaaat cggcgatacg
      781 aaccagaaat ccaactatat ccacgtcggc tcgctcaatt tcggcatgtc gtaccggttg
      841 acgcagcgcg acgtgctcaa cctcggtttc gaaatcgggg tgaccgagga tgcgcccgac
      901 gtgtcgatca cgctgcgcat gccgttcggc ggcaaatagt ttcaacagcg gccccataaa
      961 gaagccccgc caatcacggc ggggccaggt gaaccgcccc tgtcgaaacg agggcggggg
     1021 gtcgttcgga aagaccctaa ttcccgagcg ctccgacggt ctgggcgccc atcacggcgc
     1081 cgatcgagtc gctgagccgc gtcgtcgcga gcgcgtcgcg gaagccttca tagccgctga
     1141 tgtcgagcac ggcgtcgacc tgctggttca gcgagacgtt gctggcggtg ttgatcaaca
     1201 cattctggat cgcgtcggtg ctctggatca gcgccgtctg gccgttgttg gcaaggaaca
     1261 ccttctcgtc gccgacgcgc atcgagatgc cgccgctggt caagatgccg ccctggacct
     1321 gcgccgcatc gacggcggtc agcgagggcg agacgaagcg ttcgatcgtt tggccttcgg
     1381 gggtccagct gacgatcgtg cgcagcgcga gttcgtcgtt cacaaagctg cggatgtcgg
     1441 cgccgaaggc gatcaccatg ccctgaaagg caaagccgcc gcgctgcgcc gccagcacct
     1501 cgtcggacac cgtctgcgcg ccggccagcg ggtcggcatc ctcgggctcg cttgccgcga
     1561 ggagcacggg cgagagagcg agcaggagga tgcgtggtga catggtcttt ctcatttcac
     1621 ggtgccgacg cgcacatcga gcaggatctg cggggtcagc tgatattggg gggggaggtg
     1681 ggtggtgaga tcgccggccg agacgtggag cgagccgtcc ttttcgagcg gggcttgcga
     1741 ccaggggccc cagtcgcctg cgaggttgaa taaggggcgt ttcttgcccc cctccacgat
     1801 cgccagggcg atgccgttcc agtgcttttc gaaatcctcg agcgaatatt cgttgaggcc
     1861 gagcaccgaa tcgccggtga gcacgcggtc gccgcgaacg cccttgatca cgacgaaatg
     1921 cttgaagccc ttgaggtcca tcagcacgat cgtcgggcgc ttcacctgct tcaactgatc
     1981 gacggtgagc cggaagccct cggcgcgaaa gccgcgcgag gcgagatagg tcttcatgtc
     2041 gagcatcgag aaaccgacct tgcggatcgc ctcgcgatcg cccttttccc acatcgcgcg
     2101 aaaaggcatc gtttcggagg ttggcgcgcc gtaatgatag gtgagaagcg tcgcgagcgc
     2161 ggccgagccg cagctgaaat cataacgctg gcggatgacc gagcggaagg gaatgtccca
     2221 ccaggtcatc acctgaagct ggtaggtgcc gcccgtttcg ggtccggtca gccggacctc
     2281 ggcggcggcg ggtgccgcgg cgagggcgca cagcgcgccc gcgatcaagg ctcgcatggg
     2341 ccgcacggcg atttcagtcg ccgagattga tggtcaggtt catgccgctc tgggcgctga
     2401 cctgcgcgcc ggtgttgatc agcagattgc cgacaccgtt gaaattcgac agggcgaaat
     2461 cgctgagcgc gaccgatccc gcggtgtaat cgccgttcag cgtgttgccg gcgacgagcg
     2521 attccatcgt ctggttggtg atcacgagcg attggccgcc gcgttgcgag tcgagctcgc
     2581 catcgttcag catgggcgat ttgcgttcgg gccgcgccgg gatcgccggc gccgcaaggg
     2641 tctcggccgg gatagattcc atcgcgacgg ggaccaccac atcggacggg ggcagttggg
     2701 ccgataccgg cacggacaag cctgccatga tcagcagaac cgggacgatc gggggtttca
     2761 tgatgcgctc ctcgaaagcg aaacgggaag aaatggccgg cccgccagtg gtgccgggcc
     2821 ggccatccgg ggggacttat cagtcgccgc cgccgccgcc ggccggggcg ctgtcgccaa
     2881 agctcacggt gccgctcgcc gcgatgttgg tcgcagcctg ggtgttggcg ttgatgcccg
     2941 tgttccatgc ggcgttcagg atgccggcat aggccgcgaa ggcgctgccg cgcaccgagt
     3001 tgttgccgct gttgtagccg acagccgttt cggtgccgtc ttcgccatcc aggtcgacga
     3061 cttcgtcgag gttctggttg gtgttggtcg cggtcagcgt ttgcgtggcg acaaccaggc
     3121 tgttgccgtt gaacgaacca ttgccgtcat tgtcctggtc gtcgtccgag aaggtgtcgg
     3181 ccgcattgtc ctggacgaac aggtcgatca ggtcgttcac aacgacggta tcattgtcgc
     3241 tgttgttgcc cgacgcaccg gtcgtggtgt tgtcgttgtc ctggtcttga ccctgcgcga
     3301 cggcgggggc tgcggtgact gccagcgcaa gcgtggagac cgcgagcatg actttcttga
     3361 tcatgacgtc ttcctctcaa taatggggac ggctcgcgat gccgaatggc atggctgatg
     3421 cggatcctcc ccgactccgt ccgcatcggt gcgaacaagg cgatgatgct ccgcggccgc
     3481 ccgtgccgct ttgcgaattc acaatgtcgg gggcggagaa atacccagat gatcccggtg
     3541 ggtggattgc ttggatcaat ccggccggcg aaacccgggt ttcaggagag tttcgcgcgc
     3601 atcgtcgcgc gaccggcggc atcgcgcgcc cacagctttg ccgcatcacc ctcgcgcgcg
     3661 aggcagaggt cgaag
//
LOCUS       DATMSU010000026         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_67010, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000026 DATMSU010000000
VERSION     DATMSU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67010"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..>1616
                     /locus_tag="VN034_00605"
     CDS             75..>1616
                     /locus_tag="VN034_00605"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWV59125.1"
                     /translation="MTATALVERDFAVPDIRCAGCIAKLEQGLVRDRRIAAARVNFTE
                     KRVHLSCTPDTDMPDLIGAFSSLGFEAHPIGSGPDEADAEAETSRELLRAVAVSGFAM
                     MNIMLLSVSVWSGAAGATRDLFHWLSAAIALPTIAYAGRPFFRSAWRALRHRRTNMDV
                     PISIGVLLVSVMSLYETATHGAHAYFDGAVMLLFFLLCGRWLDSVMRDRARGGVTALL
                     RNMGTGAMVFGHERSTRWVDATALEPDMVMLVAAGERLAADGVIASGSSSIDLSLLTG
                     ESAPQTVRPGDVVHAGTLNLDAPLKVRVTAAGSDTAIADIARLMGEAAQGKSRYVRIA
                     DRAARLYAPAVHCLALLAFAGWMIAGAGWHHSLLIAAAVLIITCPCALGLAVPAAQIV
                     AAGALMRKGVLIKDGSALERLSEVDLALVDKTGTLTLGRPEALDLGLLDIGVKSLVLA
                     LAQSSRHPLSEALTRGLQAYDVRPAEVASIRETAGFGVEARWAGRTVTLGRPLGTGLG
                     DALATQ"
BASE COUNT          228 a          548 c          549 g          291 t
ORIGIN      
        1 gtgttggggc gctggcggct cgaccttatc gtccggcgtg gcgcggacga agcccgttat
       61 cgcgagaatc ttcaatgacc gcgaccgccc tcgtcgaacg cgacttcgcg gtgcccgaca
      121 ttcgttgcgc cggctgcatc gcaaagctgg agcaaggact ggtgcgcgac aggcgcatcg
      181 ccgccgcgcg cgtcaacttc accgaaaagc gcgtccatct gtcctgcacc cccgacaccg
      241 acatgcccga cctgatcggc gccttttcga gcctgggttt cgaagcccac ccgatcggga
      301 gcggccctga cgaagccgat gccgaagccg aaacgagccg tgagttgctg cgagcggtcg
      361 cggtgtcggg cttcgcgatg atgaacatca tgctgctctc ggtgtcggta tggtcgggcg
      421 cggcgggggc tacgcgcgac ctgttccact ggctgtcggc ggcgatcgcc ctgccgacga
      481 tagcttacgc cggacgcccc ttctttcgtt cggcctggcg cgcgctgcgt catcgccgca
      541 ccaatatgga cgtgccgatc agcatcgggg tcctgctcgt cagcgtcatg agcctctacg
      601 agacggcgac gcacggcgcc cacgcctatt tcgatggcgc agtaatgctg ctctttttcc
      661 tgctctgcgg ccgctggctc gacagcgtga tgcgcgaccg cgcgcggggc ggcgtcaccg
      721 cgctgctccg caacatgggc accggtgcga tggtctttgg gcacgaacgc tcgacgcgct
      781 gggtggacgc gactgcgctt gagccggata tggtcatgct cgtcgctgcc ggtgagcggc
      841 tcgcggcgga cggcgtcatc gcgagcggat cgagcagcat cgatctgtcg ctgctgaccg
      901 gcgaaagcgc gccgcagacg gttcgcccgg gcgacgtcgt tcatgcgggc accctcaacc
      961 tcgatgcacc gctgaaggtt cgcgtcaccg ctgcgggcag cgatactgcg atcgccgata
     1021 tcgccaggct gatgggcgag gccgcgcagg gtaagtcgcg ctatgtccgg atcgccgatc
     1081 gcgcggcccg cctctatgca ccggccgtcc attgccttgc gctgcttgcc tttgcaggct
     1141 ggatgatcgc gggcgccggc tggcatcaca gcctgctcat cgctgcagcg gtgctgatca
     1201 tcacttgccc gtgcgcactc ggccttgctg tgcccgccgc gcaaatcgtc gccgcggggg
     1261 ccctgatgcg caagggggtg ctgatcaagg atggctcggc gctggagcgc ctgtccgaag
     1321 tcgacctcgc gcttgtcgac aagaccggga cgctcacgct tggccgcccc gaggcgctcg
     1381 atctcgggct gctcgatatc ggcgtcaaat cgttggtcct cgcgctggct cagtcgagcc
     1441 gtcatccgct cagcgaagcg ctgacccgcg ggctgcaagc ctatgacgtt cgtcccgccg
     1501 aggtcgcttc gatccgagaa accgcggggt tcggggtgga agctcgctgg gccggccgca
     1561 ccgtgacgct cggtcggccg ctcggcaccg ggctgggcga tgcgctcgcg acccag
//
LOCUS       DATMSU010000027         3980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_180762,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000027 DATMSU010000000
VERSION     DATMSU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180762"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="rplO"
                     /locus_tag="VN034_00610"
     CDS             <1..504
                     /gene="rplO"
                     /locus_tag="VN034_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWV59126.1"
                     /translation="LRDNDGAPKGRMRVGRGIGSGKGKTAGRGQKGQKARSGVAINGF
                     EGGQMPLHMRIPKRGFNNIFAKDFAIVNLGMIQKLIDEKKLDAKAVIDHAALKAAGVA
                     RGGKDGVRLLAKGELTTKVSFAVAGASKGAIEAVEKAGGKVELPEVKEAAPEGKKAQR
                     RAKAKAE"
     gene            737..2110
                     /gene="secY"
                     /locus_tag="VN034_00615"
     CDS             737..2110
                     /gene="secY"
                     /locus_tag="VN034_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWV59127.1"
                     /translation="MASRADQLASNLNFSKFGQATELKNRIWFTIGALIVFRFLSFVP
                     LPGVDPVALASLYSQAASGGVLDIFNTFSGGSLERMSIIALGVMPYITASIVVQLAAA
                     LSPSLAALKKEGESGRKKLNQYTRYGTVALTAIQGYFIAVGLETLGASQGIAAVVDPG
                     MMFRVGAVISIVGGTLFLMWLGEQITSRGIGNGVSLIIMAGILAQLPRSFAQMFTQVR
                     EGSMGGGTIFAVIGGAIVIIAFISFMERAQRRVLVQYPKRATQRGVMQADRSHLPLKV
                     NTAGVIPPIFASSLLLMPLTVTQMMGQNVQGDTATGDFLITLNQYLAHGQPLYMALYA
                     AGIIFFCFFYVAVVFNPEETADNLKKQNGFIPGIRPGKNTATYLDFVLTRITVIGAAY
                     LAVICLVPEYFIAGSGLPFQLGGTSLLIIVNVTIDTISQIQSHMLAHQYGDLIKKAKL
                     KGGVARR"
     gene            2160..2819
                     /locus_tag="VN034_00620"
     CDS             2160..2819
                     /locus_tag="VN034_00620"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWV59128.1"
                     /translation="MTLNIILLGPPGAGKGTQASRLEDEHGMVQLSTGDMLRAAVKAG
                     TPIGLQAKAVMDAGELVSDAIVSGLIGERLDELGPDVSVIFDGYPRTAAQADALDTIL
                     SGRGRKLDHVIELRVEEDALVDRITGRFSCAKCGAGYHDRYKLPKVENVCDVCGSGEF
                     KRRPDDNEETVRTRMAEYRAKTAPILPIYDARGIVTHVDGMAPIDQVNDAIETILATA
                     G"
     gene            2868..3551
                     /locus_tag="VN034_00625"
     CDS             2868..3551
                     /locus_tag="VN034_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59129.1"
                     /translation="MTISRYLTGAAFVAALAFAPAASAKIIDQGEIGFTVAHTAQVAA
                     TPADVWKMLRMPQSWWSKEHSWSGDAANFWLDSQAGGCFCEKLPDAGSGVGSVQHARI
                     LFAKPNEMLRLSGAFGPLQGEALDATLTIQIKETPTGSALRFDYVVGGYMRFKVAEIA
                     PAVDKVIGEQLVGLANALGGALPPTRDEKAAEPPKEETPAAKEEPGLDAAVADLVEEE
                     AKPAPDESR"
     gene            complement(3548..>3980)
                     /locus_tag="VN034_00630"
     CDS             complement(3548..>3980)
                     /locus_tag="VN034_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWV59130.1"
                     /translation="IARRLGLVALDRFVLSADVRAIAGGIAAKGEVGADVVQACAATD
                     QPVSATIAEPFDLRFLRDVDAPASEEEEIEIGSDDLDLLPLEGDRIDLGEAAVQTLSL
                     ALDPFPRHPDATRILAEKGVLSEEAAGPFAALAKLRGKPDA"
BASE COUNT          715 a         1341 c         1294 g          630 t
ORIGIN      
        1 cttcgtgaca acgacggcgc ccccaagggc cggatgcgcg tcggacgcgg catcggctcg
       61 ggcaagggca agaccgccgg ccgcggccag aagggccaga aggcgcgcag cggcgtcgcg
      121 atcaacggct tcgagggcgg ccagatgccg ctccacatgc gcatcccgaa gcgcggcttc
      181 aacaacatct tcgcgaagga cttcgcgatc gtcaatctgg gcatgatcca gaagctgatc
      241 gacgaaaaga agctcgacgc caaggctgtc atcgatcacg ccgcgctcaa ggcggctggc
      301 gtcgcgcgcg gcggcaagga cggcgtccgc ctcctcgcca agggcgagct gaccacgaag
      361 gtcagcttcg cggtcgccgg cgcgtcgaag ggcgcgatcg aagcggtcga aaaggccggc
      421 ggcaaggttg aactgcccga agtcaaggaa gcggcgcccg agggcaagaa ggcccagcgc
      481 cgcgccaaag ccaaggcgga ataatcaaaa ttgctcccct cccgcttgcg ggaggggcag
      541 cgagacttgc gagcttgctc gctagtcgca gcggggaggg tgatgcggcc ttgcgtgagg
      601 tcgacagagg ccccacgcaa ctcctcctcc caaggagggg ctaaaatggc ctttcccctt
      661 gcgcttcgcg accgccgaac gcacataggc gccgggtcgc ggggggcgcg gtccggaata
      721 tccgaggaaa acacccatgg cctctcgcgc cgaccagctt gcatccaacc tcaacttctc
      781 gaagttcggt caggcgaccg agctcaagaa ccgcatctgg ttcacgatcg gtgcgctgat
      841 cgtcttccgc ttcctgtcgt tcgtgccgct tcccggcgtc gatccggtcg cgctggcgtc
      901 gctctacagc caggccgctt cgggcggcgt cctcgatatt ttcaacacct tctcgggcgg
      961 cagcctcgag cgcatgagca tcatcgcgct cggcgtcatg ccctatatca ccgcgtcgat
     1021 cgtcgtgcag ctggcggccg cgctgtcgcc cagcctcgcc gcgctcaaga aagagggcga
     1081 aagcgggcgc aagaagctca accagtacac gcgctacggc accgtcgcgc tgaccgcgat
     1141 ccagggctat ttcatcgccg tcgggcttga aaccctcggc gcgagccagg gaatcgcggc
     1201 ggtcgtcgat cccgggatga tgttccgcgt cggcgccgtc atcagcatcg tcggcggcac
     1261 actcttcctg atgtggctcg gcgaacagat caccagccgc ggcatcggca acggcgtctc
     1321 gctgatcatc atggcgggca ttctcgcgca attgccgcgc agcttcgcgc agatgttcac
     1381 gcaggtccgc gaaggctcga tgggcggcgg cacgattttc gccgtcatcg gcggcgcgat
     1441 cgtgatcatc gccttcatca gcttcatgga acgcgcgcag cgccgcgtgc tggtccagta
     1501 tccgaagcgc gcgacccagc gcggcgtgat gcaggccgac cgcagccacc tgcctttgaa
     1561 ggtcaacacc gcgggcgtca tcccgccgat cttcgcctcg tcgctgctgc tgatgccgct
     1621 gacggtgacg cagatgatgg ggcagaatgt ccagggcgac accgcgaccg gcgatttcct
     1681 gatcacgctc aaccagtatc tcgcgcacgg ccagccgctc tatatggcgc tctatgccgc
     1741 gggcatcatc ttcttctgct tcttctacgt tgcggtcgtc ttcaaccccg aggaaaccgc
     1801 cgacaatctc aagaagcaga acggtttcat tcccggcatc cgtcccggca agaacaccgc
     1861 gacctatctc gacttcgtgc tgacgcgcat caccgtcatc ggtgccgcct atctcgcggt
     1921 catctgtctg gtacccgaat atttcatcgc cggatcgggc ctccccttcc agctcggcgg
     1981 caccagcctg ttgatcatcg tgaacgtcac gatcgacacg atcagccaga tccagagcca
     2041 catgcttgcg catcaatatg gcgacctcat caagaaggcc aaattgaaag gcggcgttgc
     2101 gcggcgctaa gccgcccgct acggcaaacg cgacacgggc tgaggcaaca ggggcttcca
     2161 tgacgctgaa catcattttg ttgggcccgc cgggggcggg taagggaacg caggcgtcgc
     2221 gcctcgagga cgagcatggc atggtccagc tctcgaccgg cgacatgctg cgcgccgcgg
     2281 tcaaggcggg aacgccgatc ggcctccagg ccaaggcggt gatggacgcc ggcgaactcg
     2341 tctccgacgc gatcgtctcg ggcctgatcg gcgagcggct cgacgagctc ggccccgacg
     2401 tttcggtgat cttcgacggc tatccgcgca ccgcggcgca agccgacgcg ctcgacacga
     2461 tcctctcggg ccgcggccgc aagctcgatc atgtgatcga gctgcgggtc gaggaagacg
     2521 cgctcgtcga ccgcatcacc gggcgcttca gctgcgcgaa gtgcggcgcg ggctaccacg
     2581 accgctacaa gctccccaag gtggagaatg tctgcgacgt ttgcggctcg ggcgaattca
     2641 agcgccgccc cgacgacaat gaggaaacgg tgcgcacgcg catggccgaa tatcgcgcca
     2701 agaccgcgcc gatcctgccg atctacgacg cgcgcggcat cgtcacccat gtcgacggca
     2761 tggcgccgat cgaccaggtc aacgacgcga tcgaaacgat cctcgcgacg gctggctagc
     2821 aactttcccc ggggccggtt attgaggcgc caagggggaa cgggctgatg acgatttcgc
     2881 gatatttgac tggcgcggcc ttcgtcgccg cgctcgcttt cgccccggcg gcaagcgcca
     2941 agatcatcga tcagggcgaa atcggcttca ccgtcgcaca caccgcgcag gtcgccgcga
     3001 ccccggccga tgtgtggaaa atgctccgca tgccgcaaag ctggtggtcg aaggagcata
     3061 gctggtcggg cgacgccgcc aatttctggc tcgattcgca ggccggcggc tgcttctgcg
     3121 aaaagctacc cgacgccgga tcgggcgtcg gcagcgtcca gcacgcgcgg atactcttcg
     3181 ccaagccgaa cgagatgctc cgcctgtcgg gcgccttcgg tccgctgcag ggcgaggcgc
     3241 tcgatgccac gctgacgatc cagatcaagg agacgccgac cgggagcgcg ctgcgtttcg
     3301 actatgtcgt cggcggctat atgcgcttca aggtcgccga gatcgcgccc gcggtcgaca
     3361 aggtgatcgg cgaacaactc gtcggcctcg ccaatgcgct cggcggggcg ctgcccccga
     3421 cgcgcgacga aaaggcggcc gaaccgccga aggaggaaac gcccgccgcg aaggaggagc
     3481 cgggactcga cgccgcggtc gccgatctgg tcgaggaaga agcgaaaccg gcgcccgacg
     3541 aaagccgcta ggcgtcgggc ttgccgcgca gtttcgcgag cgcggcgaac ggccccgccg
     3601 cctcttcgct gagcacgccc ttttcggcga ggatgcgtgt cgcatcggga tggcgcggaa
     3661 agggatcgag cgcgagcgac agcgtttgca ccgcggcttc gccgaggtcg atccggtcgc
     3721 cctcgagcgg cagcagatcg agatcgtcgc tgccgatctc gatctcttcc tcttcgcttg
     3781 ccggggcgtc gacgtcgcgc aggaagcgca ggtcgaacgg ctcggcgatc gtcgcggaca
     3841 cgggctggtc ggtcgcggcg cacgcctgca ccacatcggc gccgacttcg cctttcgcgg
     3901 cgatcccgcc cgcgatcgca cgcacgtcag cggagaggac aaagcgatcg agcgcgacga
     3961 ggccgagccg ccgcgcgatc
//
LOCUS       DATMSU010000028         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_95451, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000028 DATMSU010000000
VERSION     DATMSU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95451"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VN034_00635"
     CDS             complement(<1..414)
                     /locus_tag="VN034_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWV59131.1"
                     /translation="MSHIGARRAARAIDALRRGWPFRIEGADGALDLLAVESARDDAL
                     VEFASHDVLLSGERAVTLKLTNQRVAATPGPVRLAGAGDTVTAALAIADPALDLANPL
                     KGPFRTIATGGEAAATAAMTMARHVGVTLPSGVRLD"
     gene            complement(411..1205)
                     /locus_tag="VN034_00640"
     CDS             complement(411..1205)
                     /locus_tag="VN034_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWV59132.1"
                     /translation="MTRTSIASWNINSVRARIGIVEKFLREEAPDILCLQETKVECTL
                     FPPEMFKRLGYEHIVTHGQRMHHGVAIVSKLPLTDVRKYDWQANGEARHVGVTLPSGV
                     RLDNVYIPAGGDIPDRDLNPKFGQKLDFFGRMTEWSATLAGMPTVLTGDFNVAPLESD
                     VWNHKALLDVVSHTPIEVETLARLQAASNWVDLGRHFIEAPTPLYTWWSYRAKDWEAS
                     NRGRRLDHMWVTPDLMAKAVSHRIVQPARSWERPSDHIPLITEFDF"
     gene            complement(1577..2203)
                     /locus_tag="VN034_00645"
     CDS             complement(1577..2203)
                     /locus_tag="VN034_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWV59133.1"
                     /translation="MTRLAAWMLAPVAVAGLAVGAPAIAQSANALASVQSHLKSTSSM
                     TADFVQTDRNGQRLSGKLTLKRPGKIRFQYQKGVPLLIVGDGSRLTMIDYEVNQVQSW
                     PVKNSPLGALLDPDRDLSKYAKVLPTGNDDVLSVEVKDPKRPEYGTITMVFVRDGAAP
                     GGLRLRGWVALDSQNNRTRIDLSNQKFNVAVADSAFRWTDPRPKRRGR"
     gene            complement(2329..>3926)
                     /locus_tag="VN034_00650"
     CDS             complement(2329..>3926)
                     /locus_tag="VN034_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HWV59134.1"
                     /translation="RAPPEIADPAQRSAPSKPRPKPQTELFTNYQLPSIDLLAPAPDR
                     PAGQIDKAALERNARLLESVLEDFQVKGVVTAVRPGPVVTMYELEPAPGTKASRVSNL
                     ADDIARNMSALSARIAPIPGRTVIGIELPNAQREAVVLHEIIGSALFQDQTGALPIIL
                     GKNISGDPMIADLAPMPHLLIAGTTGSGKSVGLNAMILSLLYRLGPDQVKMIMIDPKM
                     LELSVYDDIPHLLAPVVTEPKKAIRALKWAVEQMEDRYRMMSSLSVRNLASYNDKVRG
                     ALAKGKSLGRRVQTGYDPDTGQPVYEEETLDYAPLPQIVVVVDELADLMMTAGKEVEF
                     LIQRLAQKARAAGIHLILATQRPSVDVITGVIKANLPTRISFNVTSKIDSRTILGEAG
                     AEQLLGKGDMLYVPGGKQITRIHGPFVSDDEVRAVADHWKGQGRPDYVESVTEDPEDG
                     GFAMEGAPAGGDSAEDRMYAKACQIVAESQKASTSWLQRQLRIGYNSAARLIERMEEE
                     GIVSPPNHVGRRDVLTDQYGQPR"
BASE COUNT          620 a         1244 c         1341 g          721 t
ORIGIN      
        1 gtcgagccgc acgcccgacg gcagcgtaac gccgacgtgg cgcgccatcg tcatcgcggc
       61 ggtcgctgcc gcttcgccgc ccgtcgcgat ggtgcgaaaa gggcctttga gcgggttcgc
      121 aagatcgagc gccggatcgg cgatcgcgag tgccgcggtc accgtatcgc cggcacccgc
      181 caatcggacc ggacccggcg tcgcagcgac gcgctggttg gtgagcttga gcgtgaccgc
      241 gcgctctcct gagagcagga cgtcatggct cgcgaattcg acgagtgcgt cgtcgcgcgc
      301 gctttcgacc gcgagcaggt cgagcgcacc gtcggcgccc tcgatgcgaa acggccagcc
      361 gcgccgcagc gcgtcgatgg cacgtgccgc ccgccgggcg ccgatatggc tcaaaaatca
      421 aactcagtga tcagcgggat atggtccgac ggccgttccc agctgcgcgc cggctggacg
      481 atacggtgcg acaccgcctt cgccatcagg tcgggggtca cccacatatg gtcgagccgg
      541 cggccgcggt tcgacgcttc ccagtccttc gcgcggtagc tccaccaggt gtagagcggc
      601 gtcggcgctt cgatgaaatg gcgcccgagg tcgacccagt tcgacgccgc ctgcagccgt
      661 gcgagcgttt cgacctcgat cggcgtgtgg ctgacgacat cgagcagcgc cttgtgattc
      721 cacacatcgc tttcgagcgg cgcgacgttg aaatcgccgg tgagcaccgt gggcatgccc
      781 gcgagcgtcg ccgaccattc ggtcatgcgg ccgaagaagt cgagtttctg accgaatttc
      841 ggattgaggt cgcggtcggg aatgtcgccg cccgcgggaa tatagacatt gtcgagccgc
      901 acgcccgacg gcagcgtaac gccgacgtgg cgcgcctcgc cattcgcctg ccagtcatat
      961 ttgcgtacgt cggtcagcgg cagcttactg acgatcgcaa cgccgtggtg catgcgctgg
     1021 ccgtgggtga cgatatgctc atagcccagc cgcttgaaca tctcgggcgg gaacagagtg
     1081 cattcgacct tggtttcctg aaggcagagg atgtcaggcg cttcttcgcg aaggaatttt
     1141 tcgacgatgc cgatgcgggc gcgaacgctg ttgatgttcc acgaagcgat gctggtgcga
     1201 gtcatgcggt ggctctatcg gcgcgcgccg gggcgcgcaa cgtggtcttt gttgagttgc
     1261 cgcgtcagcg gcaaagtact ccagacaaat taaacccccg tcccaggggc ggtggaacgg
     1321 gggttcaagg tcagcgttcg tcacgctctt gaatgaaggt gcctggcaac ccgagtgggg
     1381 caacaggggg aaacccaaat ccgggggccg tgttggcgcc ttcgagagtt ggaatagccg
     1441 ctgttccctg tcgccaagct gaacaaaaag gcgcgcttcg cgccggtcct gcagttcgtc
     1501 gatagcgggc gacggttggt ctggaaaaaa atggcgccgc ccccgacggg agcgccgcca
     1561 tgcccgcccg gaccggttaa cgaccgcgcc gtttcgggcg gggatcggtc cagcggaacg
     1621 ccgaatcggc gaccgcgacg ttgaacttct ggttgctgag gtcgatccgc gtccggttgt
     1681 tctgcgaatc gagcgcgacc cagccgcgca ggcgcaggcc gcccggcgcc gcgccgtcgc
     1741 ggacgaacac catggtgatc gtgccatatt cggggcgctt cggatccttg acctcgacgc
     1801 tcagcacgtc gtcgttcccg gtcggcagga ccttggcata tttcgacaag tcgcggtcgg
     1861 ggtcgagcag cgcacccagc ggcgaattct tcaccggcca gctctgcacc tgattgacct
     1921 cataatcgat catcgtcagc cggctgccgt cgccgacgat cagcagcggc acgccctttt
     1981 gatactggaa gcggatcttg cccgggcgct tgagggtcag cttgcccgac agccgctggc
     2041 cgttgcggtc ggtctgcacg aagtcggcgg tcatcgaact ggtcgatttg aggtgcgact
     2101 gcaccgacgc cagcgcgttc gccgactggg cgatcgcggg cgcgccgacc gcgagcccgg
     2161 cgacggcaac gggtgcgagc atccaggcgg caagtcgggt catcgtcttc ttggtcattt
     2221 ggctctcatt ctcgtttttg cagtccgctt tttcgcaatc gggcattgaa cccgcgctga
     2281 accccgcggt tagacgctgt tcaagtcgcg gggcggggaa aaggttcctc agcgcggctg
     2341 gccatattgg tcggtgagca cgtcgcggcg gccgacatgg ttcggcgggc tgacgatgcc
     2401 ttcttcctcc atccgctcga tcagacgcgc ggcgctgttg tagccgatgc ggagttgccg
     2461 ctggagccag ctcgtcgagg ccttctggct ctcggcgacg atctggcagg ccttggcgta
     2521 catgcggtcc tcggcgctgt cgccgcccgc gggcgcgccc tccatcgcaa agccgccgtc
     2581 ctcgggatcc tcggtgacgc tttcgacata gtcaggacgc ccctgacctt tccagtgatc
     2641 ggcgaccgcg cgcacctcgt cgtcggagac gaaggggccg tggatgcgcg tgatctgctt
     2701 gccgccaggg acatagagca tgtcgccctt gccgagcaat tgttcggcgc ccgcttcgcc
     2761 caaaatggtg cggctgtcga tcttcgaggt cacgttgaag ctgatgcgcg tcggcaggtt
     2821 cgccttgatg acgccggtga tgacgtcgac cgagggacgc tgcgtcgcga ggatcaggtg
     2881 gatgcccgcc gcgcgtgcct tttgcgcgag acgctggatc aggaattcga cctccttgcc
     2941 cgcggtcatc atcaaatcgg cgagctcgtc gacgacgacg acgatctgcg gcagcggcgc
     3001 ataatcgagc gtctcttcct catagaccgg ctggccggtg tcggggtcgt agcccgtctg
     3061 cacgcgccgc ccgagcgact tgcccttggc gagcgcgccg cgcaccttgt cgttgtagga
     3121 ggcgaggttg cgcaccgaca gcgacgacat catccggtag cgatcctcca tctgctcgac
     3181 cgcccatttg agggcgcgga tcgccttctt gggctcagtc acgacggggg cgagcaggtg
     3241 cggaatatcg tcatagacgc tgagttcgag catcttggga tcgatcatga tcatcttcac
     3301 ctgatcggga ccgaggcgat agagcagcga caggatcatc gcgttgagac cgaccgactt
     3361 acccgacccc gtcgtacccg caatcagcag gtgcggcatc ggcgcgagat cggcgatcat
     3421 cggatcgccg ctgatattct tgccgaggat gatcggcagc gcgccggtct ggtcctggaa
     3481 cagcgcgctg ccgatgattt cgtgcagcac gacggcctcg cgctgcgcgt tgggcagctc
     3541 gatgccgatg acggtacgcc ccgggatcgg cgcgatgcgc gccgacagcg ccgacatgtt
     3601 gcgcgcgatg tcgtcggcga ggttcgacac gcggctcgcc ttggtgcccg gcgcgggttc
     3661 gagttcgtac atggtgacga ccgggccggg gcgcaccgcg gtgacgacgc ccttgacctg
     3721 gaaatcctcg agcaccgatt cgagcagccg cgcgttgcgt tcgagcgccg ccttgtcgat
     3781 ctgtcccgcc ggccggtcgg gcgcgggggc gagcaggtcg atcgagggca gctggtaatt
     3841 ggtgaacagc tcggtctgcg gcttgggccg gggtttcgag ggcgcgctac gctgcgcggg
     3901 gtcggcgatc tcgggcggcg cgcgcg
//
LOCUS       DATMSU010000029         4385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_57440, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000029 DATMSU010000000
VERSION     DATMSU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57440"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..327)
                     /locus_tag="VN034_00655"
     CDS             complement(37..327)
                     /locus_tag="VN034_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWV59135.1"
                     /translation="MKQGWIYILTNRRNGTLYIGVTSNLPQRIQQHREGLIGGFAKKY
                     GLKMIIWYEHFDNLHDARRREVQMKEWKRAWKIELIEATNPEWRDLYPDLLR"
     gene            complement(392..1456)
                     /locus_tag="VN034_00660"
     CDS             complement(392..1456)
                     /locus_tag="VN034_00660"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWV59136.1"
                     /translation="MTIGGMMTDDQDWASALEGPPKKPMRRGTKIALWLFAALVAWLT
                     LSNASFLAPDPAGKPKLIAHRGVYHLYDKRAAAGRDTCTAAHALPPSHEVFENTPESM
                     RWAVGLGANMVEVDVAPTKDGRMVLFHDWTVDCRTDGTGETRELTLAELKALDIGHGY
                     TADGGQTFPLRGKGIGKMPTVEEGLAALPVHPILFNFKSNDAAEADQLFAILTASGRD
                     SAAIGDAFYGAEAPVKRMRELMPGNWSFDLKGGAKACTKDYVTWGWAGIVPESCRGGV
                     IAIPINYQWAFWGWPDRLIQRMDSVGARVIVLGPYESGKSNEGLTTPQQLAKIPASFN
                     GYIWVEDIREVGPALRPRQK"
     gene            complement(1512..2441)
                     /locus_tag="VN034_00665"
     CDS             complement(1512..2441)
                     /locus_tag="VN034_00665"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWV59137.1"
                     /translation="MIRLDGHDRIEAPLRGAVIALGNFDGFHAGHQAVVGRAVHHAQD
                     EGLPAIVATFDPHPVRFFKPDVPPFRLTTLDQRQELFAEAGADAMLVLPFDAALAGTT
                     AEDFIAKLLLDRYGAAGVVTGADFVFGKGRGGDVVTLADHARRLGFFTEMVAPVDDAE
                     EVISSSRIREALQAGDCATATRLLTRPFIVRGLVQHGDKNGRLLGFPTANLEMGQYLR
                     PRYGIYAVTGRLPDGRILKGAANLGIRPSFDPPKELLEPHFFEFAEDLYGQEIDVAFH
                     AFIRPEARFDDMDALMAQIAADCDAAKRLLADL"
     gene            complement(2438..3631)
                     /locus_tag="VN034_00670"
     CDS             complement(2438..3631)
                     /locus_tag="VN034_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWV59138.1"
                     /translation="MRRWLIAILLILALAALAFFALAPGMIERGMNQIDGKPLPQVSA
                     RAQALHKTLTIVDLHSDTLLWKRSILDRAERGHMDLPRLEDGNVALQILASTTKSPKG
                     QNYDANSGDTDNITSLVIAQLQPVRTWNSLLERSLWHAEKLHRAVAASAGDLRAVDAP
                     EDIDALLAARRGKALPVGAMLSIEGLHVLEGRLANLDKLYAAGFRMAGLTHFFDNDLA
                     GSMHGLKKGGLTPLGRQVVETMEAKGMIVDIAHCSAACVADILKIARRPVVSSHGGVQ
                     ATCKVNRNLSDEQIRGVAATGGLIGIGYWDAAVCDTSPASIAKAMKHVRDLVGARHVA
                     LGSDYDGATTVRFDTSKLAQVTQALIDAGFSDDEIRAVMGGNAVRVLKAGLVPLTPPP
                     SISAP"
     gene            complement(3636..4127)
                     /locus_tag="VN034_00675"
     CDS             complement(3636..4127)
                     /locus_tag="VN034_00675"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWV59139.1"
                     /translation="MNRPEITLILARAANGVIGAGGKMAWHLPADLRRFKQLTMGRPM
                     IMGRKTFDSLPAILEGRRHIVITRDPEWQDEGAEPVGTIEEALKLANAPHVMVIGGAE
                     IYRLFLPLADRIELTEVALEPKGDASIDYPPAAEWRETAREDHPADEAGRPAYSFVTL
                     ARK"
     gene            complement(4124..>4385)
                     /locus_tag="VN034_00680"
     CDS             complement(4124..>4385)
                     /locus_tag="VN034_00680"
                     /EC_number="4.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040946.1"
                     /note="With PurE catalyzes the conversion of
                     aminoimidazole ribonucleotide to carboxyaminoimidazole
                     ribonucleotide in the de novo purine nucleotide
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWV59140.1"
                     /translation="NSGHWTIEGAVTSQFENHIRAVAGLPLGSTATRALPVTMRNLIG
                     AGIADVPALLADENCHVHHYSKTEVREGRKLGHATWVGASPI"
BASE COUNT          666 a         1413 c         1526 g          780 t
ORIGIN      
        1 atttgtgaac cccggcgaaa gccggggccc agaaagctag cgcaaaaggt cgggatagag
       61 atcgcgccat tcggggttcg tcgcttcaat caactcgatc ttccatgcgc gtttccattc
      121 tttcatctgg acctcgcgac ggcgggcatc gtggagatta tcgaaatgtt cgtaccaaat
      181 gatcatcttg agtccgtatt tcttggcgaa gccgccgatc aggccttcgc ggtgctgctg
      241 gatgcgttgc ggcaggttgc tggtgacgcc gatgtagagc gtcccgttgc gcctgttggt
      301 gaggatatag atccagcctt gcttcatgat gcgacgttat actgggcccc ggctttcgcc
      361 ggggatcacc ttggtccgct gtggagcagt ttcacttctg ccgcggtctg agcgccgggc
      421 cgacttcgcg gatatcctcg acccagatat agccgttgaa gctcgcgggg attttcgcga
      481 gttgctgcgg ggtggtgagg ccttcgttcg acttgccgct ttcgtatggg cccagcacga
      541 tgacgcgcgc gccgacgctg tccatccgct ggatcagccg gtcgggccag ccccagaagg
      601 cccattgata gttgatcggg atcgcgatca cgccgccccg gcagctttcg gggacgatgc
      661 ccgcccagcc ccaggtcaca taatccttgg tgcatgcctt cgcgccgccc ttgaggtcga
      721 acgaccaatt gccgggcatg agttcgcgca tccgctttac cggcgcttcg gcgccataga
      781 aggcgtcgcc gatcgcggcg ctgtcgcggc ccgatgccgt caggatcgcg aacagctggt
      841 cggcctcggc cgcgtcgttc gacttgaagt tgaagaggat cgggtggacc gggagcgccg
      901 cgaggccttc ctcgaccgtc ggcatcttgc cgatgccctt gccgcgcaag gggaaggtct
      961 ggccgccgtc ggcggtatag ccatggccga tatcgagcgc cttgagctcg gccaaggtca
     1021 gctcgcgcgt ctcgcccgtg ccgtcggtgc ggcaatcgac ggtccagtcg tggaacagca
     1081 ccatgcgccc gtccttggtc ggcgcgacgt cgacctcgac catgttcgcg ccgagcccga
     1141 ccgcccagcg catcgattcg ggggtgttct cgaacacctc gtggctcggc ggcagcgcgt
     1201 gcgccgcggt gcaggtgtcg cgcccggcgg cggcgcgctt gtcatagagg tgatagacgc
     1261 cgcggtgcgc gatcagcttg ggcttgcccg cgggatcggg agcgaggaag ctcgcgttcg
     1321 acagcgtgag ccacgcgacg agcgccgcaa agagccagag cgcgattttc gtgccgcggc
     1381 gcatcggctt tttgggcggg ccttcgagcg cgctggccca atcctgatcg tcggtcatca
     1441 tccccccgat cgtcattccg gacctgatcc ggaatccatg gaccccggat caggtccggg
     1501 gtgacgaaga attacagatc ggccagcagc cgcttcgccg cgtcgcaatc ggccgcgatc
     1561 tgcgccatca gcgcgtccat atcgtcgaac ctcgcctcgg ggcggatgaa ggcgtgaaag
     1621 gcgacgtcga tttcctgccc gtagagatcc tcggcgaact cgaagaaatg cggttcgagc
     1681 aactccttcg gcgggtcgaa gctcggacgg atgccgaggt tggcggcgcc cttcaggatg
     1741 cggccgtcgg gcaggcggcc ggtgacggcg tagatgccgt agcgcgggcg gagatactgc
     1801 cccatttcga ggttggcggt cgggaaaccg agcaggcggc cgttcttgtc gccgtgctgg
     1861 accagcccac gcacgatgaa gggacgcgtg aggaggcgcg tcgcggtggc gcaatcgccc
     1921 gcctgcagcg cctcgcggat gcggctcgac gagatcacct cctcggcatc gtcgaccggc
     1981 gcgaccattt cggtgaagaa gccgaggcgg cgcgcatggt cggcgagcgt caccacatcg
     2041 ccgccgcggc ccttgccgaa gacgaagtcg gcgccggtga cgacccccgc cgcgccgtag
     2101 cgatcgagca ggagcttcgc gatgaaatcc tccgccgtcg tgcccgcgag cgcggcgtcg
     2161 aacggcagga cgagcatcgc gtcggcgccc gcttcggcga agagttcctg ccgctggtcg
     2221 agcgtcgtca ggcggaaggg cgggacgtcg ggcttgaaga agcgcaccgg atgcgggtcg
     2281 aaggtcgcga cgatcgcggg aagcccctcg tcctgcgcat gatggaccgc gcggccgacc
     2341 accgcctgat gccccgcgtg gaagccatcg aaattgccga gcgctatcac cgcgccgcgc
     2401 aagggcgctt cgatgcggtc gtggccgtcg aggcgaatca tggcgccgaa atcgagggcg
     2461 gcggcgtcag cggcacgagc cccgctttca gcacgcggac cgcgttgccg cccatcacgg
     2521 cgcggatctc gtcgtcgctg aaccccgcgt cgatcagcgc ctgcgtgacc tgggccagct
     2581 tcgacgtgtc gaagcgcacc gtggtggcgc cgtcatagtc gctgccgagc gcgacatgcc
     2641 gggcgccgac gaggtcgcgg acatgcttca tcgccttcgc gatgctcgcg ggcgaggtgt
     2701 cgcagacggc ggcgtcccaa tagccgatgc cgatcagccc cccggtcgcg gcgacgcccc
     2761 tgatctgctc gtcggaaagg ttgcggttga ccttgcatgt cgcctgcacc ccgccgtggc
     2821 tcgataccac cgggcggcgg gcgatcttca atatgtcggc gacgcaggcg gcggagcaat
     2881 gcgcgatatc gacgatcatc cccttcgcct ccatcgtttc gaccacctgc cgcccgagcg
     2941 gcgtcagccc gccctttttc agcccgtgca tcgaccccgc gaggtcgttg tcgaagaaat
     3001 gggtgagccc cgccatgcgg aagccggcgg cataaagctt gtcgaggttg gcgagcctgc
     3061 cttcgaggac gtgcagcccc tcgatgctga gcatcgcgcc gacgggcagc gccttgccgc
     3121 ggcgcgcagc gagcagcgcg tcgatatcct cgggcgcatc gaccgcgcgc aggtcgcccg
     3181 ccgaagcggc gaccgcgcga tgcagcttct cggcgtgcca gagcgagcgt tcgagcagcg
     3241 aattccaggt gcgcaccggc tggagctgcg cgatcacgag gctggtgata ttgtcggtgt
     3301 cgccgctgtt ggcgtcgtaa ttctggccct tgggcgactt ggtcgtgctc gcgaggattt
     3361 gcagcgcgac gttgccgtcc tcgagccgcg gcaggtccat atggccgcgc tcggcgcggt
     3421 cgaggatgct gcgcttccag agcaaagtat cgctgtgcag gtcgacgatc gtcagcgtct
     3481 tgtgcagcgc ctgcgcgcgc gcgctgacct gcggcagcgg cttgccgtcg atctggttca
     3541 ttccgcgctc gatcatgccc ggagcgagcg cgaagaaggc gagcgcggcg agagcgagga
     3601 tgagcaggat agcgatcagc cagcgacgca tcggcctatt tcctcgccag cgtgacgaag
     3661 ctgtacgccg gccggccggc ctcgtcggcg ggatggtcct cacgcgccgt ctcgcgccat
     3721 tcggcggcgg ggggataatc gatgctcgcg tcgcccttgg gttcgagcgc gacttcggtc
     3781 agctcgatgc ggtcggcgag cggcaggaag aggcggtaga tttcggcgcc gccgatcacc
     3841 atcacatgcg gcgcgttggc gagcttcagc gcttcctcga tcgtgccgac cggctcggcg
     3901 ccttcgtcct gccattcggg atcgcgcgtg atgacgatat ggcggcggcc ttcgaggatc
     3961 gcgggcaggc tgtcgaaggt cttgcgcccc atgatcatcg ggcgccccat ggtgagctgc
     4021 ttgaagcggc gaaggtcggc ggggaggtgc cacgccatct tgcccccggc gccgatcacg
     4081 ccattggccg cgcgggcgag gatcagcgtg atttcgggtc gattcatatg ggagaggctc
     4141 caacccatgt ggcgtggccg agtttgcgcc cctcgcgcac ctcggtcttg ctgtaatgat
     4201 ggacgtggca attttcgtcg gcgagcagcg cggggacgtc ggcgatgccg gcgccgatca
     4261 gattgcgcat cgtgacgggc agcgcgcggg tcgcggtgct gccgagcggc aggcccgcga
     4321 cggcgcggat gtgattttcg aactggctgg tcaccgcgcc ctcgatcgtc cagtggccgc
     4381 tgttg
//
LOCUS       DATMSU010000030        11514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_180818,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000030 DATMSU010000000
VERSION     DATMSU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180818"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1756
                     /locus_tag="VN034_00685"
     CDS             <1..1756
                     /locus_tag="VN034_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV59141.1"
                     /translation="AASGGAAERISSDGAKPQFGATSDRVFMVTGADGKSQLVSTDLH
                     GQDKRVHAHGELVSDYEISPDGRTLAFRQNYDAYVTPLMPGGQDVSLGTKSAALPVTR
                     VSGSGAEYMHWSDGGRRLHWSRGATLLSADLASLFTNAPADEKAPKFTPPTDGVSLSM
                     TREASKHEGVVVITGAKIVTMADKDGGVIENGAIVIDGDRITAIGPAGAVTVPAGAVT
                     VDAAGKTIVPGFVDAHAHGSHGEDELVPQQNWSEIVNLAMGTTTSHNPSSRASEIFVS
                     SEMQRAGLILAPRIFSTGEVIYGAKAAGVYAEINGYDDALAHVRRLKAQGAHSVKNYN
                     QPRRDQRQMVVKAAQTEGMTVVPEGGSLYTQDVTLIQDGNSTVEHNVPLDIFYKDLVQ
                     LWGQTQVDYTPTLVVTYGGPAGDPYWRAHTNVWEHPILTKHIPPAELAANNKRRVIAP
                     ESDYVDDDSAREAGKIAAAGRMVSIGAHGQQSGLGAHWEIWSFVRGGWSNIDALRAAT
                     IMPATSLGYAKDVGSLEAGKLADLVILDADPTQDIRNTEKIHRVMLGGRLYDPLTMNE
                     VETGDRTRAPYWWEADKP"
     gene            1765..2598
                     /locus_tag="VN034_00690"
     CDS             1765..2598
                     /locus_tag="VN034_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59142.1"
                     /translation="MLFTEICFAGDDGCELRAWVSPPCPEIDNPTPIVLLHGGGPDHR
                     MFLPLAESMPGDFTVILPDIRGYGRSLCRDASCHRWSQYASDVIAMLDALGIARAIIG
                     GAGLGGTIALRVAVEHPGHVLAVVVIGLEDIEDDEAKQVEIRLMDEFAERARRDGIAA
                     AWEPLFPHLSPVIGTLVRDAIPRSDPASIAAAAAIGRDRSFISPHELAAIAVPTLLFP
                     GADARHPEELAKAVAKIIPKAEAAGISMSGQLETSDDFARAFGPEITGFARKIRNEID
                     G"
     gene            2697..2786
                     /locus_tag="VN034_00695"
     tRNA            2697..2786
                     /locus_tag="VN034_00695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2731..2733,aa:Ser,seq:cga)
     gene            complement(2903..7612)
                     /locus_tag="VN034_00700"
     CDS             complement(2903..7612)
                     /locus_tag="VN034_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59143.1"
                     /translation="MADLPPPPPGFELVDNPRKPKSRVRDRLSDTTPSGDVRDLGTTY
                     GATGRTIDGDTIQLQTPPNALGPLNGRLSGYDAFERGQLGYRSNGSPVALGMDSTNAL
                     AGYITPQTTVFGVGSPSYGRPVVGLNNDGQDPVNALLRSGQGYAAPQYLSADPERAAE
                     YMEAERLGRMNRQGGFGTTHVRPDAYRRPLTTRTAQLASDEYLAFNDELPDDAPKLKR
                     LSPEQDAKVAAYVVSQAGNPNFSLQQYRDWFRNELGVTDFEPVDDPKFIDSVRKGEGF
                     SPAIDYSNLDAQSMRDLKELIAFSGLRPEDAKGYRDLLAGGDAEQVFAFAKEKGMSLD
                     PDEIARYIEARQKGEAPAIPLPQINPGDGAFGAGMRGFGDTPTSGLLDELGALPDALG
                     LTGYRENVWNSDRSFGEIYDNNLRQNRAILDYDATNHPYARFGGQMVGGLVVPFGAGA
                     RGATGMAKVGGAYGGAYAFGSAEGDLGDRLKAVPGGVAIGAAGGAILGKGIEAGAPVV
                     NRLLGRGVDDAASAASPIADDLTDLPPPPPGFQIERGATRADMAADDVAPSITSDRIP
                     DVIDVNAARTRPLGDGPSPNLMRAATARVEPEDVLPRASNELTPEEAARLGEGPYRPV
                     PAPRERDYLDAREYPSRAKPDSTITRRGPADLVTFARSLNGIRDEGGELTAAGISNAP
                     RKGDDFAGGENRLGRLVNDTHGVTIEEAAQRAWSEGYFPELAGPPTREQFVAALDDTY
                     RGVNRRFRPDDEPEIQAFEGARDQRLAVERAKQEGAPLVDDLGQPTTYDDVIANTPPA
                     ISPDDWNPATLAKVGNVRVDKLDSPQEISRALKVADDITGGFDNARRGRITQAETEAL
                     ATDLGMTADDLLARRKGQALNAEEALAARRILSASGNELVNLAKRVQRAGDEPGSELL
                     AAFRKAMVRHTAIQEQVSGATAEAGRALAQFRMVASSRDHIGRVMDGLVNAGGGPGRL
                     KEAADMIITLEPDPATLNRFIEKASKPKWSDKLTEVWYNFLLSGPQTHAVNVMSNTMT
                     ALAQIPEHAVAAGIGNVRRVLSPSARQNLEDRIPFTEVGARAIGLIQGTKEGMREFAR
                     ALRTGEATDFVTKVENQSQKAVSGLKGEVLRLPTRFLTAEDELFKGIARRMELSGLAV
                     RTARQEGLRGAEGRAHAAELLANPTDDMMLRALDYARYVTFQRPLGEAASKVSAFTQA
                     LPITKIILPFVRTPTNLFKSFIEHSPGAPLLKEWRRDFAAGGARRDMAVARVLVGTGM
                     GATVAEAAARGDITGSPPTDDNKRRLMMADGWQPYSVKIGDKYYSYQRLDPFALTIGA
                     AADMATLRDGMTDNQRDKGAARIVASIMGNLSNKTWLSGVSDMLAALDDPDRFGDAFI
                     KRFAGSATVPTGVAQVARTLDPTARMTPDVKSAIQNRIPGMSDDLLPKRDVWGRPIEK
                     EGGLGPDIMSPIWTSTEKGDPATWEALRVGATISPPSDRVNGQKLDPKVYDQLQAVTG
                     PLAHEWVSELIKAPEYRSLDLDGQAKAIKFVMEKAKDAGKANVMSGVPIPTNRPVKGQ
                     RRSQRQPPPPPPGFVVQ"
     gene            complement(7619..8026)
                     /locus_tag="VN034_00705"
     CDS             complement(7619..8026)
                     /locus_tag="VN034_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59144.1"
                     /translation="MSALDYLRDASGDPVALVKLAKAALASCELEGVNPLIATVEGLT
                     FARLAAAQGDLSGFGLMVTLGAQLADLLDDVGDFDTATDMRGKALAAADVACDKVPEG
                     SLGEILGAYLAANELADPDSLPQAASARRFWGG"
     gene            complement(8023..9267)
                     /locus_tag="VN034_00710"
     CDS             complement(8023..9267)
                     /locus_tag="VN034_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59145.1"
                     /translation="MSANWGLLDPNAYRRGFEQSQDMFDQVRKEQVRKATGSALQDLM
                     ADPNGRPRSLNALAMLAPDTASQLIKFQQGQQDRSRDTEYRSALSDYYGAGGGNSMNA
                     LMPRPMRPSTPTSNIAPPPISDAPPPPADWEARLGPGGLDQLPAMSGTPHRMGNPAQP
                     ITHIDPNSVAADPYAAQGIGAESGVAGQVAPGAEETGLPMPPEIARAAQSPDMNVRNA
                     AFKRMMQLNPIEAMKIQSSERDRHLDGMEDANKAFRFAAEALGNARDDATYKQVLDRV
                     EQAVAPLGIDIRSMVPGTHPGPDGIRELQMQALDISQRFAAIDRRFSAEARVADIEAD
                     NERADRNTNSIIEDRQARTGIARDRAATSDRREARIAAGGGGRGKGGGKAPVKIRQGE
                     AVAAGPGGAKMVVRGGKWVPAQ"
     gene            complement(9279..10049)
                     /locus_tag="VN034_00715"
     CDS             complement(9279..10049)
                     /locus_tag="VN034_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59146.1"
                     /translation="MPAAVIGGVIAGAGAIGGAALSSKAQKKAASTAANAQLDATAQN
                     NALAREQYGLNQAALAPWQQRGNAAGTAVNALLGIGQTVPANNNTPAAQAAATAAQKL
                     QYDNAFKNFQKSTGYQFRLGEGQRALNTGFAGKGALNSGAAMKAMATYGQNLASGEFG
                     SYMNALTGVSNQGLSAANAQAGVGTNLVNAVSNNNNLSAGALGNQAFANANAQGQLWG
                     AAGNALGNIGSAFASSFGGGNSLNAASQRLIAANPNIF"
     gene            complement(10034..10543)
                     /locus_tag="VN034_00720"
     CDS             complement(10034..10543)
                     /locus_tag="VN034_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59147.1"
                     /translation="MLVTIPAVAHPASLLAIDSPEIMGWRTFDVGRMNYIVNHPSIRP
                     HVGGDPAQPLDFTDAIANHDNHFLSGEHGGFSCIWTAPGTYEIHTFVLPEGRGAWAAA
                     FAMWGRQYLVDLGARHLWTMVHPDAANVRTFTLKAGFEPAGSRVVDLGAGPVSYEIFD
                     WSPDRCQQQ"
     gene            complement(10543..10824)
                     /locus_tag="VN034_00725"
     CDS             complement(10543..10824)
                     /locus_tag="VN034_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59148.1"
                     /translation="MQTVPHTEPVLQAALAAHEAQRFTHDNLALLRFAHDIAQHYGLE
                     MIAVVDRLCTVPQSFLDLAHDPCGLTALASMLASELGSEVAAPFMVAVH"
BASE COUNT         2033 a         3647 c         3679 g         2155 t
ORIGIN      
        1 cgccgcgagc ggcggggcgg ccgagcggat cagcagcgac ggcgccaagc cgcagttcgg
       61 cgcgacgagc gaccgcgtgt tcatggtcac cggcgccgac ggcaagagcc agctcgtgag
      121 caccgacctt catgggcaag acaagcgcgt ccacgcccat ggcgaactcg tcagcgatta
      181 cgagatttcg cccgacggcc gcaccctcgc cttccggcag aattacgacg cctatgtcac
      241 gccgctgatg cccggcggac aggatgtgtc gctcggcacc aagagcgccg cgctccccgt
      301 gacccgcgtt tcgggcagcg gcgccgaata tatgcactgg tcggatggtg gccgccgcct
      361 ccactggagt cgcggcgcga cgctgctcag cgcagacctc gccagccttt tcaccaacgc
      421 acccgccgac gaaaaggcgc cgaaattcac cccgccgacc gacggcgtct cgctgtcgat
      481 gacgcgcgag gcctcgaagc atgaaggtgt cgtcgtcatt accggcgcca agatcgtgac
      541 catggccgac aaggacggcg gcgtgatcga aaatggcgcg atcgtgatcg acggcgaccg
      601 catcaccgcg atcggcccgg ccggcgcggt cacggttccg gcgggcgcgg tgacggtcga
      661 cgcggcgggc aagacgatcg tccccggctt cgtcgatgcc cacgcgcacg gatcgcacgg
      721 cgaagacgaa ctggtgccgc agcagaactg gtctgagatc gtcaacctcg cgatgggcac
      781 gacgaccagc cacaatccct cgtcacgcgc gtcggagatt ttcgtgtcgt ccgaaatgca
      841 gcgcgcgggc ctgatcctcg cgccgcgcat cttttcgacc ggcgaggtca tctatggcgc
      901 gaaggcggcg ggggtctatg ccgagatcaa cggctatgac gacgcgcttg cgcacgttcg
      961 ccgcctgaag gcgcagggcg cgcacagcgt caagaattac aaccagccgc gccgcgacca
     1021 gcggcagatg gtggtgaagg cggcacaaac ggaaggcatg accgtcgtgc ccgagggcgg
     1081 ctcgctctat acgcaggacg tgacgctgat tcaggacggc aattcgaccg tcgagcacaa
     1141 tgtcccgctc gacatcttct acaaggacct cgtccagctg tggggccaga cgcaggtcga
     1201 ttacaccccg acgctggtcg tgacctatgg cgggcccgcc ggcgatccct attggcgcgc
     1261 gcatacgaac gtctgggagc atccgatcct gacgaagcat atcccgccgg ccgaactcgc
     1321 cgcgaacaac aagcgccgcg tgatcgcccc cgaaagcgac tatgtcgacg atgattcggc
     1381 gcgcgaggcg ggcaagatcg ccgcggcggg ccgcatggtg tcgatcggtg cgcacggcca
     1441 gcaatcgggg ctcggcgcgc attgggaaat ctggtcgttc gtgcgcggcg gctggagcaa
     1501 tatcgacgcg ctgcgcgccg cgacgatcat gcccgcgacc tcgttgggct atgcgaagga
     1561 cgtcgggtcg ctcgaggcgg gcaagctcgc cgatctggtg attctcgacg ccgatccgac
     1621 gcaggacatc cgcaataccg agaaaatcca ccgcgtaatg ctcggcgggc ggctctatga
     1681 tccgctgacg atgaacgagg tggagacggg cgatcgcacg cgcgcaccct attggtggga
     1741 ggctgacaag ccctgatctg aaaaatgctg ttcaccgaaa tctgtttcgc aggtgatgat
     1801 ggatgcgagc tgcgcgcatg ggtttcgccg ccctgcccgg agatcgacaa tccgactccg
     1861 atcgtccttc tccacggcgg gggccccgat catcggatgt tcctgccgct ggcggagagc
     1921 atgcccggcg atttcacggt catcctcccc gacattcgcg gttatggccg gtcgctgtgc
     1981 cgcgacgctt cgtgccaccg gtggagccaa tatgcgtccg atgtcatcgc catgctggac
     2041 gcgctgggca ttgcgcgtgc catcataggc ggcgccgggc tcggcggaac catcgccctg
     2101 cgggttgcgg tcgagcaccc cggacatgtt ttggcggtgg tggtgatcgg actcgaggat
     2161 atcgaggatg acgaggcgaa gcaggttgaa attcgtttga tggacgaatt cgccgagcgg
     2221 gccagacggg acggcatagc ggcggcgtgg gagccgcttt ttccgcattt atcgccagtc
     2281 atcggaacgc tggtccgcga cgccattccg cgatccgatc cggcaagcat cgccgcagcc
     2341 gccgcgatcg ggcgtgatcg atccttcata agtccgcacg aacttgccgc gatcgccgtt
     2401 cccacgctcc tgttcccggg agcagatgct aggcatcccg aagaattggc aaaggcggta
     2461 gcaaaaatca tcccgaaggc cgaggctgcc gggatttcaa tgtcgggcca gctggaaacc
     2521 agcgacgatt ttgcccgcgc ctttggacct gaaatcacag gctttgcccg caaaatccgg
     2581 aacgagatcg acggataaca gcccgcctgc tatccgggca tgatagaaat cgcccttttg
     2641 cgactagagg gttgcaacat catcggaccc ccgctagggg cgtctcctct cagcgaggag
     2701 agttggctga gtggtcgaaa gcgtcgcact cgaaatgcga agtgccggca acggtaccga
     2761 gggttcgaat ccctcactct cctccatttc tttcccttag tggactgaaa acgctagcct
     2821 ttcgaccggg cgagcaaaat cgttccccca ttcgtgccca cataaggttt tctttatgcc
     2881 ggggtcgccc tttcgggagc gatcattgca cgacaaagcc gggaggcggt ggcgggggct
     2941 gacgctgcga acgacgctga ccctttactg ggcgattcgt cgggatcggc acgcctgaca
     3001 tcacgttggc ctttccggcg tccttggcct tctccatcac gaacttaatc gccttcgctt
     3061 gcccgtccaa atcgagcgag cgatattccg gcgccttgat aagctcgctc acccattcgt
     3121 gggcaagcgg cccggtgacg gcctgcaact ggtcatagac tttcggatcc agcttttgac
     3181 cgttcacgcg atccgacggg ggcgagatgg tggcgccaac gcgcagagct tcccatgtcg
     3241 ccgggtcgcc cttctcggtt gaggtccaga taggcgacat gatgtcaggg ccaagcccgc
     3301 cctctttttc aatcgggcgc ccccacacat cccgctttgg cagcagatcg tcggacatgc
     3361 cggggattcg attttgaatt gccgatttca catcgggggt catgcgcgcg gtggggtcca
     3421 gtgtgcgggc gacttgcgca acgccggtcg gcacagtcgc cgaaccagca aaccgcttga
     3481 tgaaggcatc gccgaagcgg tcggggtcgt cgagcgcggc cagcatgtcc gacacgcccg
     3541 agagccaagt cttattcgac aggttgccca tgatgctcgc gacgatccgc gcggcgccct
     3601 tgtcgcgctg gttatcggtc atcccgtcgc gcagcgttgc catgtccgcg gctgcaccga
     3661 tcgtcagcgc gaagggatcg agacgctgat agctgtaata cttgtcgcca atcttgaccg
     3721 agtagggttg ccagccgtcc gccatcatca gccggcgctt attgtcatcg gtcggcggtg
     3781 agccggtaat gtccccgcgc gcagcagctt ccgcgacggt ggctcccatg ccggtgccga
     3841 ccagcacccg agcaaccgcc atgtcgcggc gcgcgccacc ggcagcgaag tcccggcgcc
     3901 actctttgag cagcggcgcg ccgggcgaat gctcgatgaa gctcttgaac aggttggtcg
     3961 gcgtgcggac gaaagggagg ataatcttgg tgatcggcag cgcctgggtg aatgctgaca
     4021 ccttcgacgc ggcttcgccc aagggacgct gaaacgtgac gtaacgcgca tagtcgagcg
     4081 cgcgcaacat catgtcatcc gtcgggttgg cgagcagctc agcagcatgc gcgcggccct
     4141 ctgccccccg caatccctcc tgccgcgcag ttcggacagc aaggccgctc aattccatgc
     4201 ggcgggcaat gcctttgaaa agttcatcct ctgccgtcag gaaccgagtg ggcaggcgca
     4261 gcacttcacc cttgaggccg ctaaccgcct tctggctttg gttctcaacc ttcgtcacaa
     4321 agtcggtggc ctcacccgtg cgcagggcgc gggcaaattc gcgcatcccc tctttcgtcc
     4381 cctgtatcag cccaatggcg cgcgccccga cctcagtgaa cggaatccga tcctcgagat
     4441 tttggcgggc agaaggcgac aatacgcggc gcacgttccc gataccggca gcaacggcat
     4501 gttcgggaat ctgcgcaagc gccgtcatgg tgttggacat gacgttgacc gcgtgcgtct
     4561 gcggacccga caggaggaag ttgtaccaaa cctcagtcag cttatccgac cacttcggct
     4621 tgctcgcctt ctcgataaat ctgttcagcg tggcggggtc aggctccagc gtgattatca
     4681 tgtcggccgc ttctttcagc cgaccgggac cgccgcccgc attgacgagg ccatccatca
     4741 cgcgcccgat gtgatcgcgg ctgctcgcca ccatgcggaa ctgcgcaagc gcgcggccag
     4801 cttccgccgt cgcgccgctc acctgttcct gaatagccgt gtgccgcacc atcgccttgc
     4861 ggaaggccgc cagcaattcc gagccgggtt catcgcctgc acgctggacg cgcttcgcga
     4921 ggttcaccag ctcattgcca gaggccgaca ggatgcgccg cgctgccaac gcctcctctg
     4981 cgttgagcgc ctgcccctta cggcgggcga gcagatcgtc ggcggtcatc ccgaggtcgg
     5041 ttgcgagggc ttcggtttct gcctgtgtga tgcggccccg gcgcgcattg tcgaaaccgc
     5101 ccgtaatgtc gtcggcaacc ttgagcgcgc ggctgatttc ttgggggctg tccagcttgt
     5161 cgacgcgaac gttcccgacc ttggcaagtg tcgcggggtt ccagtcatcg ggcgagatgg
     5221 caggcggcgt gttggcgatc acatcgtcat atgtcgtcgg ttgcccgagg tcgtcgacca
     5281 acggggcgcc ttcctgcttg gcgcgctcca cggcaaggcg ctgatcgcgg gcgccctcga
     5341 atgcctgaat ttcgggttcg tcgtcaggac ggaaccgccg attgacgccg cgataggtat
     5401 catcgagcgc ggcaacgaat tgctcacggg tcggcggccc cgccagttca gggaaatagc
     5461 cctcgctcca tgcgcgctgc gcggcttcct caatcgttac gccgtgggtg tcgttcacca
     5521 gtcgcccaag gcggttctca cccccggcga agtcatcgcc cttgcggggc gcgttgctga
     5581 taccggcagc ggtcaactcg ccgccctcgt ctcggatgcc gttcaacgag cgcgcgaagg
     5641 tcacaaggtc ggcaggaccg cggcgcgtga tcgtgctgtc aggtttcgcc ctggagggat
     5701 attcgcgcgc gtcgagataa tcacgctcgc ggggtgcggg aacaggccgg taaggcccct
     5761 cgcccaaccg ggccgcttct tccggcgtta gttcgttcga cgcgcggggc aacacgtctt
     5821 ccggctcaac gcgggcagtc gcggcgcgca tcaggttggg cgacgggcca tcacccagcg
     5881 gacgtgtgcg agctgcgttg acgtcgatga catcggggat gcggtcggac gtgatcgacg
     5941 gcgccacgtc atcggcggcc atgtcggcac gggtagcgcc acgctcaatt tggaaaccgg
     6001 gaggcggcgg cggaaggtcg gtgagatcgt cagcgatcgg cgaagcggcc gatgcggcat
     6061 catcgacgcc gcgaccgagg aggcggttca cgactggcgc gcctgcctct atgcctttgc
     6121 cgaggatcgc gccaccagca gcgccgatag ccacgccacc gggtacggct ttcaatcggt
     6181 cgccaagatc accctctgcg gaaccgaagg cataggcacc gccataggcg ccgccgacct
     6241 ttgccatgcc agtagcaccg cgagcgccag cgccgaacgg cacaacaagg ccgccgacca
     6301 tctgcccacc gaagcgggca taagggtgat tggtcgcgtc gtaatcgagg atagcgcgat
     6361 tctggcgcag gttgttgtcg tagatttcac caaaggagcg gtcactgttc cagacgttct
     6421 cacgatagcc agtcagtcct agtgcatcgg gcaaagcgcc aagctcatcg agaaggcccg
     6481 aggtcggagt atcgccaaag ccgcgcatcc cggccccgaa tgcgccatca cccggattaa
     6541 tctgcggcag cgggattgct ggcgcctcgc ccttctggcg cgcttcgatg tatcgcgcga
     6601 tttcgtcggg atctagggac atgccctttt ccttggcgaa agcgaaaacc tgttcggcat
     6661 cgccgccggc caataggtcg cggtagccct tggcgtcctc tggccgcaac ccggagaagg
     6721 cgataagctc tttcaggtcg cgcatggatt gcgcatcaag gtttgaatag tcgatggctg
     6781 gcgagaagcc ctcgcccttc cgcacactat cgataaattt gggatcgtcg accggctcga
     6841 agtcggtcac gccaagctca ttgcggaacc agtcgcgata ttgctggagc gaaaagttcg
     6901 gattgccagc ctgcgacacg acataggcag caaccttcgc gtcctgttcg ggagacagcc
     6961 ttttcagctt cggcgcgtcg tccggcaatt cgtcgttgaa ggcgagatat tcgtcggagg
     7021 cgagttgcgc ggttcgcgtc gtgagcgggc gccgataggc gtcagggcga acatgggtgg
     7081 tgccgaagcc accttgccgg ttcatccgcc cgaggcgttc tgcttccata tattcggcgg
     7141 cgcgctcagg atcggcgctc aggtattgcg gcgctgcata gccctgccct gaccgcagca
     7201 gcgcgttcac cgggtcttgg ccatcattgt tcaagccgac taccgggcga ccatagctcg
     7261 gcgagccgac gccaaagaca gtcgtttgcg gagtgatgta gcccgcaagc gcgttcgtcg
     7321 aatccatgcc caaggcaacg ggcgaaccgt tcgagcgata gccgagctgc ccgcgctcga
     7381 atgcgtcata cccgctaagc cgtccattga gggggccgag ggcattgggc ggggtctgta
     7441 gctggatcgt gtcgccgtcg attgtgcgac cggttgcgcc gtaggtggtc ccaaggtcgc
     7501 gcacgtcacc cgagggcgtg gtatcgctga gccggtcccg aacgcgggac tttggcttgc
     7561 ggggattgtc taccagttca aagccgggag gcggcggcgg cagatcggcc aaggtctatc
     7621 accctcccca aaagcggcgg gcgcttgcgg cttggggaag gctatccggg tcggcaagct
     7681 cattggctgc gagatatgcg ccgaggattt cgccaaggga accctctggc accttgtcgc
     7741 acgcaacgtc ggcggcggcg agcgccttgc cccgcatgtc ggttgcggtg tcgaagtcgc
     7801 ccacgtcgtc gagaaggtcg gcgagctgtg cgccgagcgt taccatcagg ccgaagcccg
     7861 acagatcacc ttgggcggca gcgaggcgcg cgaaggtcag cccttcgact gtggcaatca
     7921 gcgggttcac cccttccagt tcgcacgaag cgagcgcggc cttggcgagc ttgaccagtg
     7981 cgaccggatc acccgacgca tcgcggagat agtctagcgc gctcattgtg cgggcaccca
     8041 cttgccgccg cgaacaacca tcttcgcacc gccggggcca gccgcgacgg cctcgccctg
     8101 tcggattttc acgggtgctt tgccgcctcc tttgccccga ccgccgccac cggcagcaat
     8161 gcgggcttcg cgtcggtcgg aggtcgcggc acggtcgcgg gcaataccgg tgcgcgcctg
     8221 ccggtcctcg ataatcgagt tggtgttgcg gtcggcgcgt tcgttgtcgg cttcgatgtc
     8281 ggccacgcgg gcctctgccg aaaagcgacg atcgatcgcg gcaaaccgct gcgaaatatc
     8341 aagcgcctgc atctgcaatt cgcggatgcc atcggggccg ggatgcgtgc cggggaccat
     8401 gctgcgaatg tcgatgccga gcggggcaac cgcctgctct actcggtcaa gcacctgctt
     8461 ataggtcgca tcgtcgcgcg cgttaccgag ggcttcggct gcgaaccgaa acgccttgtt
     8521 cgcatcctcc atcccgtcaa gatggcggtc gcgctccgaa ctttggatct tcatcgcctc
     8581 gataggattg agctgcatca tgcgcttgaa agccgcattg cggacattca tgtcaggact
     8641 ttgcgcggcg cgcgcgatct cgggtggcat gggcaggcca gtctcttccg cacccggtgc
     8701 gacctgtccg gctacccccg attcggcacc gatgccctgc gcagcgtagg ggtctgcggc
     8761 aacgctattc ggatcaatgt gagtgatagg ctgcgccggg ttgcccatgc ggtgcggcgt
     8821 cccactcatt gcgggaagct ggtcaaggcc acccggccca agccgagcct cccaatcggc
     8881 cggcggcggt ggcgcatcgc tgatcggggg cggcgcaatg ttgctggtcg gcgtgctcgg
     8941 gcgcatcggg cgaggcataa gggcgttcat gctgttgccg ccacctgcgc cgtaatagtc
     9001 cgacagggca ctgcgatatt cagtgtcccg cgaacgatcc tgctgaccct gctggaactt
     9061 gataagttgc gaagccgtgt cgggcgccaa catggcaagc gcgttgaggc tccggggacg
     9121 tccattcggg tccgccatca ggtcttgcag cgccgagccg gtcgccttcc gcacctgttc
     9181 cttgcgcacc tgatcgaaca tatcttgcga ctgctcaaag ccgcgacggt atgcgttcgg
     9241 atcgagaagg ccccaatttg cgctcatggt gttgctcctc agaaaatatt ggggttggca
     9301 gcgatgagcc gctgtgacgc ggcgtttagc gagttgccgc cgccgaacga gctggcgaac
     9361 gccgatccga tgttgccgag cgcattcccc gccgctcccc acaactgacc ttgcgcattc
     9421 gcattggcaa aagcctgatt gcccagagcg cccgcggaga gattgttgtt gttcgacacg
     9481 gcgttgacga gattggtacc gactcccgcc tgcgcgttcg ccgcggatag cccctgattg
     9541 ctgacgccgg tcaaagcgtt catgtaactg ccaaattcgc cgctcgccaa gttttggccg
     9601 taggtcgcca tagccttcat ggcggccccg gaattgagcg cacccttgcc ggcgaacccc
     9661 gtattgagcg cccgctgacc ttccccaagg cggaactggt agccggtgct cttctggaaa
     9721 ttcttgaagg cgttgtcgta ctgcaatttc tgcgccgctg tggcggcggc ctgagcggcg
     9781 ggcgtgttgt tgttagccgg caccgtctgt ccaattccaa gcagcgcgtt gaccgcagtg
     9841 ccagcggcat tgcctcgctg ctgccacggg gcaagagcgg cttggttgag cccgtattgc
     9901 tcgcgggcca aggcgttgtt ctgcgcggtt gcgtcaagct gggcattggc ggcggtgctg
     9961 gccgctttct tctgggcctt cgacgacagc gccgcgccac caatggcacc ggccccggcg
    10021 atcacgcccc caattactgc tgctggcatc ggtcaggact ccaatcgaaa atctcatagg
    10081 acaccggacc cgcgccaaga tcgacgacgc gggaaccggc aggctcgaac cctgctttca
    10141 gggtgaaggt gcggacgttg gcagcgtcag ggtgaaccat cgtccagaga tggcgcgcgc
    10201 cgaggtcgac gagatattgc cgcccccaca tggcgaaggc tgcggcccat gccccccgcc
    10261 cttccggcag gacgaacgtg tgaatctcgt aggtgccggg cgcggtccag atgcaggaga
    10321 acccgccatg ctcgccggaa aggaaatggt tgtcatggtt tgcaatcgca tcggtgaagt
    10381 cgagaggctg ggccggatcg ccgccgacgt gcgggcggat cgacggatga ttgacgatgt
    10441 agttcatgcg gcccacgtcg aacgtccgcc agcccatgat ttcggggcta tcgatcgcaa
    10501 ggaggctcgc gggatgcgcg acagcgggga tcgtcaccag catcaatgca ccgccaccat
    10561 gaacggcgcg gccacttcgc tgcccaactc actcgcgagc atcgaggcaa gcgcagtcag
    10621 gccgcacgga tcatgcgcca gatcgaggaa agattgcggg accgtgcaga gccgatcaac
    10681 caccgcgatc atttcaaggc cgtagtgctg ggcaatgtca tgggcgaagc ggagcagcgc
    10741 gagattgtca tgcgtgaacc gctgggcttc gtgagcagcc aatgcggcct gtaggacagg
    10801 ctcagtgtgc gggactgtct gcaaggttcg cctcccagtt gtcacggcgc cgaccagcga
    10861 gcgcggggca tggaacaggg agcgggtggc gtcggacccc ctgccaataa tctttgattt
    10921 cgctgatcga cgccatgacg ggtacgtcgg tatcataata cccgtgaaaa ggcaaggaaa
    10981 tcgcgttatt tccgtgactt agcttaatct atcgtgcgcg attgttctct atacttaagg
    11041 gaatggcccg cgctaggagg cgtccagaaa ctcgcccgat cctgaccgcc atactgtctc
    11101 gcaaaaatgc ccgacggtcg atattttcaa ccttcgcgcc gggaaggttg atcttccggt
    11161 cgccgccgcg ttcttcccgc gtattatcgc gggcgaggct tcccgtattt tcccgtaaat
    11221 acggtgtggc agcagatccc cgcggttgat cgtcccgagc ctatttctgc ctattttatt
    11281 ggcacgcctc gacaatggcc ggcgcctctg tttcccctct gtattatcag tgagcgggca
    11341 tggctgtttc ccgcctgtaa gatcagccag cgaggcccct tttacggggt gaaaaaattc
    11401 ggccatcgag cctaacgcgc gcacccgttt tctggcgcgg gaaaatcggc cgatcaggtt
    11461 tcccggtggt tataataacc agcgccgtcg tcgcttcgac cttggggtaa tctt
//
LOCUS       DATMSU010000031         7653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_114412,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000031 DATMSU010000000
VERSION     DATMSU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7653
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114412"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1189)
                     /gene="hisC"
                     /locus_tag="VN034_00730"
     CDS             complement(74..1189)
                     /gene="hisC"
                     /locus_tag="VN034_00730"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWV59149.1"
                     /translation="MTDATETLVPKPWISGIAPYVPGKSAGADGRPLIKLSANENPLG
                     TGEKARAAFAAAQTAPDALSRYPDPGSVELRETIAAKFGLDPARIICGNGSDELLHLA
                     AGTYAGVGDEILYVRYGFAVYEIAARRVGAVPVEADDRDYATDVDALLAAVTDKTRVV
                     YLANPNNPTGTLATREDVARLYAGLPKNVLFVIDQAYSEYLSEAEDDGGLELAKTQPN
                     VFITRTFSKIHGLAAERIGWGYAAAEVISALHRIRLPFNVTRAGQAAAVAALGDEEFV
                     AHSREHNATWRAWLAGELESLGNHGVRVVPSACNFLLVLFEGDVSAETVYNRLMDAGY
                     IVRWLPGQGLPHALRMTIGTEDETRGLAAAIRAALNG"
     gene            1281..2405
                     /locus_tag="VN034_00735"
     CDS             1281..2405
                     /locus_tag="VN034_00735"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWV59150.1"
                     /translation="MASLLHQIQHQGVTINAPLPLDSGQVLPSVTIAWQSYGALNADR
                     SNAVLICHALTGDQYVASDHPGTGKPGWWARMVGPGKPIDTDRFHVICANVLGSCMGT
                     SGPATPDAATGAPLGMAFPVITITDMVRAQAMLLDHLGISRLHAVVGGSMGGMQTLAW
                     AATYPERLQSALVIASAARHSAQNIAFHEVGRQAIMADPDWQDGQYYGSARAPTKGLA
                     VARMAAHITYLSEEGLTEKFGRRLQARDIKSFGFDADFQVESYLRHQGLAFTDRFDAN
                     AYLYITRAMDYFDLADPHDGRLAGAFANAKDVRFTLVSFDTDWLYPTSESRRVVQALQ
                     SVGAAASFVELSAPFGHDSFLLDVPALDRLVAGSLRAGLT"
     gene            2402..2839
                     /locus_tag="VN034_00740"
     CDS             2402..2839
                     /locus_tag="VN034_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV59151.1"
                     /translation="MTRAYRLSFEPADMQVEAIHAFLAQSYWAENIARAVVERAIAGS
                     LCVGVFAPGGEQVGFARVVTDRATFAYLADVYVLEAHRGHGLAARMVTALHDHPELQG
                     LRRWMLATSDAHGLYAALGWSPIEDSTPFMQRHFPDVYRQAAR"
     gene            2836..3423
                     /gene="metW"
                     /locus_tag="VN034_00745"
     CDS             2836..3423
                     /gene="metW"
                     /locus_tag="VN034_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HWV59152.1"
                     /translation="MKLRPDLAIIADTVPSTARVLDVGCGDGELMAALRAKGVDARGL
                     EIDPDNVTAAIARGQSVVQGDANRDLADYPDDAFDYAILSQTLQTTERPDRVVDELLR
                     IAPRAFVSFPNFAHWRIRLALLWGGRMPVTRLLPVAWYETPNIHHVTVSDFRDLVRAK
                     GIRVERVWHLSGDKPASDAAASWRAEHAIFLISRT"
     gene            complement(3547..4182)
                     /locus_tag="VN034_00750"
     CDS             complement(3547..4182)
                     /locus_tag="VN034_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV59153.1"
                     /translation="MDQPRTETGEGAGDARDKTPRTERGRRTLRKLLDAAAIEFGERG
                     FHEASISAITRRAGTALGSFYTYFDSKEEIFQALVRYMSEQLRDHVTPLVQAAPDEIS
                     AERIGLESYLGFVREHKEIYRIIDEAEFVDYASYRSHYETTVARVRQRLENGAARGEI
                     RGDVSEIHAWAIGGMNVFLGMRYGLWDTESDISEIAAIANDLLANGLKKRD"
     gene            4444..6705
                     /locus_tag="VN034_00755"
     CDS             4444..6705
                     /locus_tag="VN034_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59154.1"
                     /translation="MIAFAPPAFAQEAGATDAGDNEIIVTARRRDERLIDVPVAITAY
                     SGEALEKAGAIDITDVGQTTPNTTLEVSRGSNSTLSAFIRGVGQQDPVSGFEQGVGIY
                     LDDVYLNRPQAAVLDIYDVERIEILRGPQGTLYGRNTIGGAVKYVTKQLPEEFSLKIK
                     GTYGTYNQADLVVTASAPIGDLLRVGGSIARLSRGGFGDNLTTGTENYNKDVWAGRGT
                     VELGGYGAPVLIRISGDYSKDKSDPRGGHRLITSLATGAPVLDNVFDTRGGLNDPKQD
                     IEAYGLAMNVTAELSDTVTLRSISAWRKDRSATPIDFDALPAVDVDVPGFYFNEQISQ
                     EFQLLYEGDRLKGLLGFYYLDATADTLFDVRIFNTFAGLTAFTEADVDTTTFAVFGDA
                     TFDFTDRLSLSVGGRYTWDKREAFILRQNYLGGGSPVFGGAGVPFGAPSTNFDGSRSF
                     NKFTPRASLSFKPTPEHTLYASYSQGFKGGGFDPRGVGVNAPAATPGSPTDAEVSAFL
                     SFLPEQVDSYEVGYKGSLLDGALNVALAAFYADYTDVQIPGSVACTVAGLPTFCGVVS
                     NAGKATFKGLEFEGRARLGEDLAAVGDRLTLSTALGYIDAQYKEYITNIGGVPTDVAD
                     FREVQNTPKWTASANLAYSAPIGDGDISFGTTLSYRSKTYQFEIPNPYIDQKGFALWD
                     ASIVYTAPDDRWSLGVFGKNLTDKLYKTSGYTFVNFDPVTGAPSSALGTEGTLTAFYG
                     NPRQVFVTGTVKF"
     gene            complement(6960..>7653)
                     /locus_tag="VN034_00760"
     CDS             complement(6960..>7653)
                     /locus_tag="VN034_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59155.1"
                     /translation="MGSVEQMQAGPAALAAAAGVPVASVEYRLAPENPFPAPQEDCHS
                     ALSWLAERAGVFGFDANRIVVAGESAGGGLAAALTIMARDLGGPAIAGQLLTYPMLDH
                     RTGGDACPYANPATGEFIWTRASNRFGWRALQGDYKADDARRGWFSPSLAEDLTGLPP
                     AYIATGSLDLFFDENLDYARRLVAAGVPVDLHSYAGAIHAFNAIPDAALSQRFNGGLL
                     AAAAAMTGPAGG"
BASE COUNT         1270 a         2691 c         2401 g         1291 t
ORIGIN      
        1 cgctcgtcga tcgcgcgcgc gatcgaggag ccgatcaggc ccagcccgac gatcgcgacg
       61 cgatcaatcg ccatcagccg ttcagcgcgg cgcggatcgc cgctgcgagc ccgcgcgtct
      121 cgtcctcggt gccgatcgtc atgcgcagcg catggggcag cccctgcccc ggcagccagc
      181 gcacgatata gcccgcgtcc atcaaccggt tgtatacggt ctcggcgctc acatcgccct
      241 cgaacagcac gagcaggaag ttgcacgccg acggcacgac gcgcacgcca tggttgccga
      301 ggctttcgag ctcgcccgcc agccacgcgc gccatgtcgc attatgctcg cggctgtgcg
      361 cgacgaattc ctcgtcgccg agcgccgcga ccgccgccgc ctgccccgcg cgcgtgacgt
      421 tgaacggcag gcggatgcgg tgcagcgccg aaatcacctc ggcggcagca tagccccagc
      481 cgatgcgttc ggcggcgagc ccgtggatct tcgaaaaggt gcgcgtgatg aacacgttgg
      541 gctgcgtctt ggcgagttcg agcccgccat cgtcctcggc ttccgacaga tattcgctat
      601 acgcctgatc gatcacgaac aggacattct tcggaaggcc cgcatagagc cgcgcgacgt
      661 cctcgcgcgt cgcgagcgtg ccggtcggat tgttcggatt ggcgagatag acgacgcgcg
      721 tcttgtcggt caccgccgcg agcaacgcgt cgacgtcggt cgcataatcg cggtcgtcgg
      781 cctcgaccgg caccgcgccg acgcgccgcg ccgcgatttc gtagaccgcg aagccgtaac
      841 gcacatagag aatctcgtcg cccaccccgg cataggtgcc cgccgcgagg tggagcagct
      901 cgtccgaccc gttgccgcag atgatccgcg cgggatcgag cccgaacttc gccgcgatcg
      961 tctcacgcaa ttcgaccgaa cccggatcgg gatagcgcga cagcgcatcg ggtgcggtct
     1021 gcgccgcggc aaacgccgcc cgcgccttct cacccgtgcc gaggggattt tcgttcgccg
     1081 agagcttgat cagcgggcgc ccgtcggcgc cggccgactt gccgggaaca tagggagcga
     1141 tgccgctgat ccacggcttg ggcacaaggg tttcggtcgc gtcggtcatg ccgcgccgtt
     1201 tagcggctgt gcgccccaag tccagcaccg agtccagcac gttgacacgc tccccgcacc
     1261 cgcttagccc cgcgcgaacg atggcgagcc ttctccacca gattcagcat cagggcgtga
     1321 cgatcaacgc accgctgccg ctcgacagcg ggcaggtgct gccgtcggtg acgatcgcct
     1381 ggcaaagcta tggcgcgctc aatgccgaca ggtcgaacgc ggtgctgatc tgccacgcgc
     1441 tcaccggcga ccaatatgtc gcgagcgacc atcccggaac cgggaaaccc ggctggtggg
     1501 cgcgcatggt cggccccggc aagccgatcg ataccgaccg ctttcacgtt atctgcgcca
     1561 atgtgctcgg tagctgcatg gggacgagcg gccccgcgac ccccgacgcg gcgaccggcg
     1621 cgccgctcgg catggccttt cccgtcatca cgatcaccga tatggtccgc gcgcaggcga
     1681 tgctgctcga ccatctcggc atatcgcgcc tccacgccgt cgtcggcggg tcgatgggcg
     1741 ggatgcagac gctcgcctgg gcagcgacct atcccgagcg cctccaatcg gcattggtca
     1801 tcgcgagcgc ggcgcgccat tcggcgcaga atatcgcgtt ccacgaggtc gggcggcagg
     1861 cgatcatggc cgaccccgac tggcaggacg gccaatatta tggcagcgcg cgcgcgccga
     1921 ccaagggcct cgcggtcgcg cgcatggcgg cgcacatcac ttatctctcc gaggaagggc
     1981 tgaccgagaa gttcggccgc cgcctgcagg cgcgcgacat caagagcttc ggcttcgacg
     2041 ccgattttca ggtcgaatcc tatttgcggc atcaggggct ggctttcacc gaccgcttcg
     2101 acgccaacgc ctatctctat atcacgcgcg cgatggacta tttcgacctc gccgacccgc
     2161 acgacggacg gcttgccggc gcgttcgcga acgcgaagga cgtgcgcttc acgctcgtca
     2221 gcttcgacac cgactggctc tatccgacct ccgaatcgcg ccgcgtcgtc caggcgctgc
     2281 aaagcgtcgg cgcggcagca agcttcgtcg aactgtcggc gcccttcggc cacgacagct
     2341 tcctcctcga cgtccccgcg ctcgaccggc tcgtcgcggg ctccttgcgg gcgggtctga
     2401 catgacccgc gcctaccgcc tgtccttcga gcccgccgac atgcaggtcg aagcgatcca
     2461 cgcctttctg gcgcaaagct attgggccga aaatatcgcg cgcgccgtcg tcgagcgcgc
     2521 gattgccggg tcgctctgcg tcggcgtctt cgcgcccggc ggcgagcagg tcggctttgc
     2581 ccgcgtcgtc accgaccgcg cgaccttcgc ttatctcgcc gacgtctatg ttctcgaggc
     2641 gcatcgcggg cacggcctcg ccgcgcgcat ggtgaccgcg ctccatgatc atcccgagct
     2701 gcagggcctg cggcgctgga tgctcgcgac cagcgacgcg cacggcctct atgccgcgct
     2761 cggctggtct ccgatcgagg atagcacgcc gttcatgcag cggcatttcc ccgacgtcta
     2821 ccggcaggcc gcgcgatgaa gctgcgcccc gatctcgcga tcatcgccga taccgttccg
     2881 tcgaccgcgc gcgtgctcga cgtcggctgc ggcgacggcg aactgatggc ggcgctccgc
     2941 gccaagggcg tcgacgcgcg cgggctcgag atcgatcccg acaatgtcac cgcggcgatc
     3001 gcgcgcggcc agtcggtggt acagggcgac gccaaccgcg acctcgccga ctatcccgac
     3061 gacgccttcg actatgcgat cctgtcgcag acgctgcaga ccaccgagcg ccccgaccgc
     3121 gtggtcgacg aactgctgcg catcgcgccg cgcgccttcg tcagctttcc caatttcgcg
     3181 cattggcgca tccggctcgc gctgctgtgg ggcgggcgga tgccggtgac gcggctgctc
     3241 cccgtcgcct ggtacgaaac gccgaatatc caccatgtga cggtcagcga cttccgcgac
     3301 ctcgtccgcg ccaagggcat cagggtcgag cgcgtctggc acctgtcggg cgacaagccg
     3361 gcaagcgacg ccgccgcgag ctggcgcgcc gaacatgcga tcttcctgat ttcgcggacc
     3421 tgattcccct ccctccaagg gaggggttag gggtgggtcg ccgcaggcgt tcttcttaaa
     3481 agaccggaag cgaggccttg cttcgtcaac ccacccccgg cccctccctt tcagggaggg
     3541 gagatatcaa tcccgcttct tcagaccgtt cgcgagcaga tcgttcgcga tcgcggcgat
     3601 ctccgaaatg tcgctctcgg tatcccacag cccatagcgc atccccagga acacgttcat
     3661 cccgccgatc gcccacgcgt gaatctcgct gacgtcgccg cggatctcgc cgcgcgccgc
     3721 gccattctcc agccgctgcc gcacgcgcgc gaccgtcgtt tcataatggc tgcgatagct
     3781 cgcataatcg acgaactcgg cctcgtcgat gatgcggtaa atctccttat gctcgcgcac
     3841 gaagccgaga taggattcga gcccgatgcg ttcggcgctg atctcgtcgg gcgccgcctg
     3901 aaccagcggc gtgacatggt cgcgcagctg ctcgctcata tagcggacga gcgcctggaa
     3961 gatttcctcc tttgaatcga aataggtgta gaagctgccg agcgcggtcc cggcgcggcg
     4021 cgtgatcgcg ctgatcgacg cttcgtgaaa gccgcgctcg ccgaattcga tcgccgcggc
     4081 gtcgagcagc ttgcgcagcg tccgccgccc gcgctcggtg cgcggcgtct tgtcgcgcgc
     4141 atcgcccgcg ccttctcctg tttcggtccg cggctgatcc atggctgcct ttccgtctcc
     4201 ccctccgtcc ggctccgtct gcatcgtggc atttccgcac cactggcaag caaaaaaaga
     4261 cgaagtcagt ttccaagttg aaacttggtt catgtttcat tattggaagc ggcgcggctt
     4321 ggcaagcacc ggcccaagaa aagcaaccgg gcggcgaggg gtcgcccatc acatgggaga
     4381 gttttcatgc gtcctttcgc ccgcccgctg caccgtgccg tgctcgccac cagcgcgctt
     4441 tcgatgatcg ccttcgcccc gcccgccttc gcgcaggaag cgggcgccac cgacgccggc
     4501 gataacgaga tcatcgtgac ggcacgccgc cgcgacgagc ggctgatcga cgtccccgtc
     4561 gcgatcaccg cttattcggg cgaggcgctc gaaaaggcgg gcgcgatcga catcaccgat
     4621 gtcggccaga cgaccccgaa cacgacgctc gaagtgtcgc gcgggtcgaa ttcgacgctc
     4681 agcgccttca tccgcggcgt cggccagcag gatccggtgt cggggttcga acagggcgtc
     4741 ggcatctatc tggacgacgt ctatctcaac cgcccgcagg cggcggtgct cgacatctac
     4801 gacgtcgaac gcatcgaaat cctgcgcggg ccgcagggca cgctctatgg ccgcaacacg
     4861 atcggcggcg cggtcaaata tgtgaccaag cagctgccgg aggaattttc gctcaagatc
     4921 aagggcacct acggtaccta taatcaggcc gacctcgtcg tcaccgcgag cgcgccgatc
     4981 ggcgacctgc tccgcgtcgg cggctcgatc gcgcgcctgt cgcgcggcgg ctttggcgac
     5041 aatctcacca ccggcaccga aaattataac aaggacgtct gggccggccg cggcaccgtc
     5101 gagctcggcg gctatggcgc gccggtgctg attcgcattt cgggcgacta ttcgaaggac
     5161 aagagcgatc cgcgcggcgg ccaccgcctg atcacgtcgc tcgcgacggg cgccccggtg
     5221 ctcgacaatg tcttcgacac gcgcggcggc ctcaacgatc cgaagcagga catcgaggct
     5281 tatggcctcg caatgaacgt caccgccgaa ctcagcgaca cggtgacgct gcgatcgatc
     5341 agcgcgtggc gcaaggaccg ttcggcgacc ccgatcgact tcgacgcgct gcccgcggtc
     5401 gacgtcgacg ttccgggctt ctacttcaac gaacagatca gccaggaatt ccagctgctc
     5461 tacgaaggcg accggctcaa gggcctgctc ggcttctatt atctcgatgc gaccgccgac
     5521 actttgttcg acgtgcgcat cttcaatacc ttcgccgggc tgaccgcttt caccgaagcc
     5581 gacgtcgaca ccacgacctt tgccgtcttc ggcgatgcga ccttcgattt caccgatcgg
     5641 ctcagcctgt cggtcggcgg ccgctatacc tgggacaagc gcgaagcgtt cattctgcgc
     5701 cagaattatc tcggcggcgg atcgccggta ttcggcggcg ccggcgtgcc tttcggcgcg
     5761 ccgagcacca atttcgacgg tagccgcagc ttcaacaaat tcaccccccg cgcttcgctg
     5821 tcgttcaagc cgacccccga gcacacgctc tacgcaagct attcgcaagg cttcaagggt
     5881 ggcggtttcg acccgcgcgg cgtcggcgtc aatgcgcctg ccgccacccc gggcagtccg
     5941 accgacgccg aagtctccgc cttcctgagc ttcctccccg aacaggtcga cagctatgag
     6001 gtcggctaca agggcagcct gctggacggc gcgctcaacg tcgcgctcgc ggccttttac
     6061 gccgattata ccgacgtcca gatcccgggc tcggtcgcgt gcaccgtggc gggcctgccg
     6121 accttctgcg gcgtcgtgtc gaacgccggc aaggcgacct tcaaggggct cgaattcgaa
     6181 ggccgcgccc ggctcggcga ggatctggcg gcggtgggcg atcggctgac gctgtcgacc
     6241 gcgctcggct atatcgatgc gcaatataag gaatatatca ccaacattgg cggcgtgccg
     6301 accgatgtcg ccgatttccg cgaggtccag aatacgccga aatggaccgc gagcgccaac
     6361 ctcgcctatt cggccccgat cggcgacggc gacatcagtt tcgggacgac gctgtcgtac
     6421 cgcagcaaga cctatcagtt cgaaattccg aacccctata tcgaccagaa gggcttcgcg
     6481 ctgtgggatg caagcatcgt ctacaccgcg cccgacgacc gctggagcct cggggtgttc
     6541 ggcaaaaatc tgaccgacaa gctatataag acctcgggat atacgttcgt caatttcgac
     6601 cccgtgaccg gcgcgccgtc ctcggcgctc ggcaccgagg gcacgctgac cgccttctac
     6661 ggcaacccgc gccaggtctt cgtcaccggc acggttaaat tctgatcttc ggccaagggg
     6721 gagaaagggg cgtccatcgc ggcgcccctt tttctattgc gcccggacgg cgagacggcc
     6781 tgatgtcggc tttggggtgg ggagcggacg ttctccgcac caacgtgatc cccggcgaaa
     6841 gccggggccc agtgcggaaa tacgctaagt tcgcgataga agtctggacc ccggcttccg
     6901 ccggggcgca catctctatc ggcagctacc ggccgaaagc ggacacccag tcatccctct
     6961 cagccgcctg ccggccccgt cattgccgcc gccgccgcga gcagtccgcc gttgaaacgc
     7021 tgcgacaagg ccgcatcggg aatcgcgttg aaggcatgga tcgcgcccgc gtagctgtgg
     7081 aggtcgacgg gcacgcccgc cgcgaccagc cgccgcgcat agtcgagatt ttcgtcgaag
     7141 aagaggtcga ggctgcccgt cgcgatatag gcgggcggca ggcccgtcag atcctcggct
     7201 aggctcggcg aaaaccagcc gcgccgtgcg tcgtcggcct tgtagtcgcc ctgcagcgca
     7261 cgccagccga agcggttgct cgcgcgcgtc cagatgaatt cgccggttgc cggattggca
     7321 taggggcagg cgtcgccgcc cgtccggtga tcgagcatcg gataggtcag aagctggccg
     7381 gcgatcgcgg gtccgccgag gtcgcgcgcc atgatcgtaa gcgcggcggc gagcccgccg
     7441 ccagcgctct cgcccgcaac cacgatccgg ttcgcgtcga agccgaacac accggcccgt
     7501 tccgcgagcc agctcagcgc cgaatggcaa tcctcttgcg gcgcggggaa agggttttcg
     7561 ggcgcgaggc gatattcgac cgacgcgacc gggaccccgg ccgccgcagc cagcgcggcg
     7621 gggccagcct gcatctgctc gaccgatccc atc
//
LOCUS       DATMSU010000032         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_142764,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000032 DATMSU010000000
VERSION     DATMSU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142764"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="VN034_00765"
     CDS             complement(<1..964)
                     /locus_tag="VN034_00765"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWV59156.1"
                     /translation="MPLKGIRVLDFGRYIAGPYCAALLADYGADVIRIEAPGGNDDRY
                     TVPVAEDGSGAMFMQMNRAKRGLTLKPGSAEGREIVRRLVETADVVIANLPHDALVKL
                     GLDYDSISAINPRIILATASAFGSEGPLASRVGFDAVGQAMSGTVHLTGTPDQPYRAQ
                     VNYVDFGTALHCAFGIMLALREREATGKGQCVSGSLLGTALAMSNALTIDHALNGIDR
                     QPIGNRAFSSGPTDLFRTRDGWIVTQIVGSGIFARWAELVGRPELIDDPRYASDILRG
                     DNGEELSAIMQRWCIERTSEDAIAELAAGRVPAAPVLRAGEALAQ"
     gene            1106..>2549
                     /locus_tag="VN034_00770"
     CDS             1106..>2549
                     /locus_tag="VN034_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV59157.1"
                     /translation="MARFALALAATLACSTSVWAQAAPDADTKKEDKWDVNAPPGLAT
                     RKVQLSVDEGSWMNVDVAPDGRTIAFDLLGDIYTMPIEGGTPTRIAEGLAFEHQPRFS
                     PDGKRIAFVSDRAGGDNIWLMNRDGSDKRQISKEDFRLLNQPSWSPDGQFLVAKKHFT
                     TGRSLGTGEVWMYHVSGGAGVPLVKKPNEQHQKELGEPIFAADGKSVFYTRNVTPGPI
                     FEYAQDSNTDLFHIENYSLDDGEISTAASGPGGAVRPTPSPDGKRLAFVRREGTQSKL
                     YVKDLASGVEQKVYDALDQDVQETWAVTGVYPNMAWTPDSRDIVFWAGGKLRRVSGNG
                     GEARIIPFSINDDRVIVDATHPAVEVAPDSFATKMPRWAEVSPDGRSVVFETLGKLWV
                     KPATGGTARRLTSAKDGAMELWPSWSRDGKSVVFVRWTDAGLGEIHVTGAGGGASRKV
                     TANPGHYAEPRVSPDGKTIVFERRGGGGLTS"
BASE COUNT          438 a          864 c          840 g          407 t
ORIGIN      
        1 gctgcgcaag cgcctcgccc gcgcgcagca ccggcgccgc gggcacccgt ccggccgcca
       61 gttcagcgat cgcgtcttcg cttgtgcgct cgatgcacca gcgctgcatg atcgcgctca
      121 gctcctcgcc attgtcgccg cgcaaaatgt cgctggcgta gcgcggatcg tcgatcagtt
      181 cggggcgccc gacgagttcg gcccatcgcg cgaagatgcc gctgccgacg atctgggtaa
      241 cgatccagcc gtcgcgcgtc cggaacaggt cggtcggccc cgagctgaag gcgcggttgc
      301 cgatcggctg gcggtcgatg ccgttcagcg catggtcgat cgtcagcgcg ttcgacatcg
      361 cgagcgcggt gccgagcagc gatcccgaca cgcattgccc cttccccgtc gcctcgcgtt
      421 cgcgcagcgc gagcatgatg ccgaaggcgc agtggagcgc ggtgccgaaa tcgacataat
      481 tgacctgcgc gcgatagggc tggtcgggcg tgccggtcag atgcaccgtg cccgacatcg
      541 cctgcccgac cgcatcgaag ccgacgcggc tggcgagcgg cccttcgctg ccgaacgccg
      601 atgccgtcgc gaggatgatg cgcgggttga tcgcggaaat actatcgtaa tcgaggccca
      661 gtttgaccag cgcatcgtgc gggagattgg cgatcacgac atcggcggtt tcgacgaggc
      721 ggcggacgat ctcgcgcccc tccgcgctgc cgggcttcag cgtcaggccg cgcttggcgc
      781 ggttcatctg catgaacatc gcgcccgatc cgtcttcggc gaccggcacc gtatagcgat
      841 cgtcgttgcc gccgggcgcc tcgatgcgga tcacatcggc gccgtaatcg gcgagcagcg
      901 ccgcgcaata gggcccggcg atataacgac cgaaatccag taccctgatc ccttttagcg
      961 gcacgcctcg ccctcccggt cattgtcttt cgaccgggct agcagccgcc cgtcgagcct
     1021 gccagcgcct tcgtcgaaag ctgccgcttg catgttcgcg cccgggccat atgctcggca
     1081 cataatttca aacggggata atttcatggc gcgcttcgct ctcgccctcg cggcgacttt
     1141 ggcttgttca acctcggtct gggcacaagc cgcgcctgac gccgacacga agaaggaaga
     1201 taagtgggac gtcaacgccc cgccgggcct cgcgacgcgc aaggtccagc tgtcggtcga
     1261 cgagggaagc tggatgaacg tcgacgttgc gcccgacggc cgcacgatcg ccttcgatct
     1321 tttgggcgac atctacacca tgccgatcga aggcgggacg ccgacgcgga tcgccgaggg
     1381 actcgccttt gaacatcagc cgcgcttctc acccgacggc aagcgcattg cctttgtctc
     1441 cgaccgcgcg ggcggcgaca atatctggct gatgaaccgc gacggcagcg acaagcgcca
     1501 gatctcgaag gaggatttcc gcctgctcaa ccagccgagc tggtcgcccg acggccagtt
     1561 cctcgtcgcc aagaagcatt tcaccaccgg ccgctcgctc ggcaccggcg aggtgtggat
     1621 gtatcatgtg tcgggcggcg cgggcgtgcc actggtcaaa aagcccaatg aacagcatca
     1681 gaaggaattg ggcgagccga tcttcgccgc cgacggcaag agcgtcttct atacgcgcaa
     1741 cgtcacgccg gggccgatct tcgaatatgc gcaggacagc aacaccgacc tgttccatat
     1801 cgaaaactac agcctcgacg atggcgaaat cagcaccgcc gcgtcggggc ccggcggcgc
     1861 ggtccgcccg accccttcgc ccgacggcaa acgcctcgcc ttcgtgcggc gcgagggcac
     1921 gcagtcgaag ctttacgtca aggacctcgc ctcgggcgtc gagcagaagg tctatgacgc
     1981 gctcgatcag gatgtgcagg aaacctgggc ggtcaccggg gtctatccga atatggcgtg
     2041 gactcccgac agccgcgaca tcgtcttctg ggccggaggc aagctgcgcc gcgtgagcgg
     2101 caacggcggc gaggcacgga tcattccgtt cagcatcaac gacgaccgcg tgatcgtcga
     2161 tgcgacgcat ccggcggtcg aggtcgcgcc cgacagcttc gcgacgaaaa tgccgcgctg
     2221 ggccgaagtg tcgcccgacg gccgcagcgt cgtcttcgaa acgctcggca agctctgggt
     2281 caagcccgcg accggcggca ccgcgcggcg gctgacgagc gccaaggacg gcgcgatgga
     2341 gctgtggccg agctggtcgc gcgacggcaa gtcggtggtc ttcgtgcgct ggaccgatgc
     2401 cggtctgggc gaaatccacg tcaccggcgc gggcggcggc gcgtcacgca aggtgacggc
     2461 gaaccccggc cattacgccg aaccgcgcgt ctcgcccgac ggcaagacga tcgtgttcga
     2521 acggcgcggc ggcggcgggc tcacctcgg
//
LOCUS       DATMSU010000033         4823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_76563, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000033 DATMSU010000000
VERSION     DATMSU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76563"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN034_00775"
     CDS             complement(<1..389)
                     /locus_tag="VN034_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV59158.1"
                     /translation="MYKQARPHQKRRKRSGAEVREEGLAAARGLLLDRGPAAVTLANV
                     GQAIGMSHANVLHHFGSAAGLQSALMESMVRDLTDALGNVVEMMKTDAAAPRAVADRV
                     FDAFDKGGAGPLAAWIILSGDVEHLEPV"
     gene            550..1407
                     /locus_tag="VN034_00780"
     CDS             550..1407
                     /locus_tag="VN034_00780"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWV59159.1"
                     /translation="MTIPLRTPDDIRIEKRDLKFGRENPPPRWWHSGDPGRTAFFNAL
                     SSTFPVGEKFFMTSVRHYREGTPQPLRGQIDDFLYQESMHSREHVVFNRQAEDAGFDI
                     ASAEDRARRTIAWVKRRSPIQQLAATCALEHFTATLAHDALANPAHLGGAAEEARQLW
                     RWHAIEEIEHKAVAFDTYLHAARTMTPLRRWLKRSTVMCVTTMRFHYVIFRNTADLLA
                     QDGRNNWATWKTLLAWLYGRDGLMRALLRGVFTYMKPGFHPWELDDRPLLEEALRLLE
                     AGKARELAA"
     gene            1404..1817
                     /locus_tag="VN034_00785"
     CDS             1404..1817
                     /locus_tag="VN034_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HWV59160.1"
                     /translation="MKRALLLLAATAFATPAAAADITGVWATGSEGGRVEIYRCGKAL
                     CGKVVDAKRLRANPDLRDVRNSDPQLRHRKLKGLVVLNGFTGGPTEWKGGPVYDPESG
                     DGAKNGRLKLLPGGKLELKGCIAFFCRTKIWTRAG"
     gene            complement(1837..2595)
                     /locus_tag="VN034_00790"
     CDS             complement(1837..2595)
                     /locus_tag="VN034_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV59161.1"
                     /translation="MKHSVDVLINDRPVMALQDEYPAGFVDPMHSHDHIQILYASSGV
                     MSVRTPETSFVIPPQRAVWLPAGTRHEVACRGPVSLRTLYLPCAGHEHERQCRVFEVS
                     NLVKSLILEVIDFPALYDVDGREGRIIALLLDEIGRMPNAPYQVSMPSNPRLLRVCNA
                     IIADPSDPRDIDEWAALAAMGRRTFTRSFKQETGMGLAVWRQQVRLMEALSLLAAGAS
                     ITQVTYDVGYDSPSGFAAMFRRAFGVPPSQYLKH"
     gene            2773..3729
                     /locus_tag="VN034_00795"
     CDS             2773..3729
                     /locus_tag="VN034_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59162.1"
                     /translation="MSKGLSAVQDDMADPDIRHFVDAINAAYAEHAAPAGASMAVRRQ
                     IAERVRRPWREGGPIMAETREMNMGGVRLRLHRPVADEKLPVMLYIHGGGWMLFSIDT
                     HDRLMREYAARAGIAVIGIDYSLSPESKFPVALEECAAALDWIAAQAGALNLDADRVL
                     IGGDSAGANLSVATCLLQRERGRPLPAAMLLNYGAFAPERTPSYARFGAGNYSLESDE
                     MDAFWAAYVDGPEQLADPLVAPLRADLTGLPPVFLAIAECDILADCNHEFTRRLEEAG
                     VPTHAVTYKGATHSFLEAMSIAPLANRALDEQAAWIRDIVKA"
     gene            3726..4304
                     /locus_tag="VN034_00800"
     CDS             3726..4304
                     /locus_tag="VN034_00800"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWV59163.1"
                     /translation="MTSLYEPRDAADVRQLIADYPLTWLVSRDFHASPLPLIAETDGS
                     GAVTALFGHCARRNPLVADFRADPRGLILFTGPEAYVPTALLSQPDWAPTWNYAVLRF
                     TVDIEFVEGETNDAIEQLVEKMEDGAWSTASLGARYDKMLDQIIAFRAHVRSAEHSFK
                     LGQDESAGGFAEIVAGHSDRTLAAWMEGQAKR"
     gene            4301..>4823
                     /locus_tag="VN034_00805"
     CDS             4301..>4823
                     /locus_tag="VN034_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HWV59164.1"
                     /translation="MKLFFDDRQMAHAPSRELHNGDWVPYAENIERPRSIVEAFTDWR
                     PVRDFGMAPLLAVHDADYLAFLERAHGDWIAAGRNGDAIGYTFPIRRRRPLDFGRIDA
                     DLGAYSYDAGTPIASGTWESAYWSAQCALSALDELAWGDARHAFALCRPPGHHAGRDY
                     MGGYCYLNNAAIAG"
BASE COUNT          845 a         1684 c         1497 g          797 t
ORIGIN      
        1 acgggttcga gatgctcgac atcgcccgac aggatgatcc acgccgcgag cgggcccgcg
       61 ccgcccttgt cgaaagcgtc gaacacgcgg tcagccaccg cgcgcggcgc cgcggcatcg
      121 gtcttcatca tttcgacgac attgccgagc gcgtcggtca ggtcgcgcac catcgattcc
      181 atcagcgccg attgcagtcc cgccgccgac ccgaaatgat ggagcacgtt cgcgtgcgac
      241 atgccgatcg cctgccccac attggcgagt gtcaccgcgg ccggcccgcg atcgaggagc
      301 agcccgcgtg ccgccgccag cccctcctcg cgaacctcgg cccccgaacg cttgcgccgt
      361 ttctggtgcg gccgcgcttg cttgtacatg ccctattccc ttgcggcccc gcgccccgcg
      421 acagccttcg cccgaaagcc ttacccgaaa gcggccgatt gtcaccctca tcgtcccgct
      481 tgccgtttcg gaaaacgcgt gatatataca ttgatgttaa tgtaactttg atcgcagaaa
      541 ggttgcacga tgaccattcc cctccgcact cccgacgaca tccggatcga aaaacgcgac
      601 ctcaaattcg ggcgtgagaa cccgccgccg cgctggtggc acagcggcga tcccggccgt
      661 accgcctttt tcaacgcgct gtcctcgacc ttcccggtcg gcgagaaatt cttcatgacc
      721 tcggtgcgcc attatcgcga gggcacgccg cagccgctgc gcggccagat cgacgatttc
      781 ctctatcagg aatcgatgca cagccgcgaa catgtcgtgt tcaaccgcca ggccgaggat
      841 gcgggcttcg acatcgcatc ggccgaagat cgcgcgcgcc ggacgatcgc ctgggtcaag
      901 cgccgctcgc cgatccagca gctcgccgcg acctgcgcgc tcgaacattt caccgcaacg
      961 ctcgcgcacg acgcactcgc gaaccccgcg catctcggcg gggcggccga ggaagcgcgg
     1021 cagctgtggc gctggcacgc gatcgaggaa atcgagcaca aggccgtcgc cttcgacacc
     1081 tatctccacg ccgcgcggac gatgaccccg ctgcgccgct ggctcaagcg cagcacggtg
     1141 atgtgcgtga cgacgatgcg ctttcactat gtcatcttcc gcaacaccgc cgacctgctc
     1201 gcgcaggacg gccgcaataa ttgggcgacg tggaaaaccc tcttggcatg gctctatggc
     1261 cgcgacggac tgatgcgcgc gctgctgcgc ggcgtcttca cctatatgaa accgggcttt
     1321 catccgtggg agctcgacga ccggccattg ctcgaagagg cgctccgcct gctcgaagcc
     1381 ggcaaggccc gggagctggc ggcatgaagc gggccctgct tcttctcgcc gccacggcct
     1441 tcgcgacgcc cgccgccgcg gccgacatca ccggcgtctg ggcgaccggc agcgagggcg
     1501 ggcgcgtcga aatctatcgc tgcggcaagg cgttatgcgg caaggtcgtc gacgccaaaa
     1561 ggctgcgcgc caacccggac ctccgcgacg tgcgcaattc ggacccccaa ttgcgtcacc
     1621 gcaagctcaa aggccttgtc gtcctcaacg gcttcaccgg cggccctacc gagtggaagg
     1681 gcggtcccgt ctacgatccc gaatcgggcg acggcgccaa gaatggccgg ctcaaactgc
     1741 ttcccggcgg caagctcgaa ctcaagggct gcatcgcctt tttctgccgc accaagatct
     1801 ggacgcgcgc cggttgacgc ggcgcactcc gccgggtcag tgtttcagat actggctcgg
     1861 cgggacgccg aaggcgcggc ggaacatcgc ggcgaaaccg ctggggctgt catagccgac
     1921 gtcataggtg acctgcgtga tcgacgcgcc ggcagccaat agcgataacg cctccatcaa
     1981 ccgcacctgc tgccgccaca cggcgagccc catccccgtt tcctgcttga agctgcgcgt
     2041 gaaggtgcgc cgtcccatcg ccgcgagcgc cgcccactcg tcgatgtcgc gcgggtccga
     2101 cgggtcggcg atgatcgcgt tgcagacgcg cagcaggcgc gggttcgacg gcatcgacac
     2161 ctgatagggc gcgttcggca tccgcccgat ctcgtcgagc agcaatgcga tgatccgtcc
     2221 ctcgcgcccg tcgacgtcat aaagcgccgg aaaatcgatg acctcgagga tcaaggattt
     2281 gacgagattc gacacttcga acacgcggca ttgccgctca tgctcgtgcc ccgcgcaggg
     2341 cagatagagc gtgcgcagcg agaccggacc gcgacacgcg acctcgtgcc gcgtccctgc
     2401 gggaagccac accgcgcgct gcggcgggat gacaaagctc gtctcgggcg tgcgcaccga
     2461 catcaccccc gacgaggcgt agaggatctg gatatggtcg tggctgtgca tcggatcgac
     2521 gaagcccgcg ggatattcat cctgcagcgc catcaccgga cggtcgttga tcagaacatc
     2581 gacactatgc ttcatggccc gatcctcgcg agtcttggac aagtgatcaa tataggccat
     2641 gcgcttcgct ggccagcgat gatggcttcg ctggcctgtc cgctgcggtg attggcccgt
     2701 tcgggcgata gatggggcgg cgcagcggct atcaggcgat caaggacagt ttccgcaaag
     2761 gatatatcga atatgtcaaa gggcttgagc gccgtgcagg acgacatggc cgaccccgat
     2821 atccgccatt tcgtcgacgc gatcaacgcc gcctatgccg aacatgccgc ccctgccggc
     2881 gcgagcatgg cggtgcgccg ccaaatcgcc gaacgcgtcc gccgaccatg gcgcgagggt
     2941 ggcccgataa tggcggagac gcgcgaaatg aatatgggcg gcgtccgcct gcgcctccac
     3001 cgcccggtcg cggacgaaaa gctgcccgtg atgctctata tccacggcgg cggctggatg
     3061 ctgttcagca tcgacacgca cgaccggctg atgcgcgaat atgccgcgcg cgccggtatc
     3121 gcggtgatcg gcatcgatta cagcctgtcg cccgaaagca aattccccgt cgcgcttgag
     3181 gaatgcgccg ccgcgctcga ctggatcgcg gcgcaggccg gcgcgttgaa cctcgatgca
     3241 gaccgcgtgc tgatcggcgg cgattccgcc ggcgccaatc tgtcggtcgc gacctgtctg
     3301 ttgcagcgcg agcgcggccg tccgctgccc gccgcgatgc tgctcaacta tggcgccttc
     3361 gctcccgaac gcacgccgag ctacgcgcgc ttcggcgcag gcaactattc gctcgaatcc
     3421 gacgagatgg acgccttctg ggccgcctat gtcgacggac ccgaacaact cgccgatccg
     3481 ctcgtcgcac cgctgcgcgc cgacctgacg gggctcccac ccgtttttct ggcgatcgcc
     3541 gagtgcgaca tccttgccga ctgcaaccat gaattcaccc gcaggctcga agaagccggc
     3601 gtcccgaccc acgccgtcac ctataagggc gcaacgcaca gcttcctcga agccatgtcg
     3661 atcgcgccgc ttgcgaaccg cgcgctcgac gagcaggcgg cgtggatccg tgatattgtg
     3721 aaagcatgac atcgctctac gaaccacgcg acgccgccga cgtccgccag ctgatcgccg
     3781 actatccgct gacatggctc gtctcgcgcg actttcacgc gagcccgctg ccgctgatcg
     3841 ccgaaaccga cggaagcggc gcggtgacgg cgctgttcgg tcattgcgcg cggcgcaacc
     3901 cgctcgtcgc cgatttccgc gccgacccgc gcgggctgat cctcttcacc ggccccgaag
     3961 cctatgtccc cacggccttg ctgtcgcaac ccgactgggc gccgacgtgg aattatgcgg
     4021 tgctgcgctt cacggtcgac atcgagttcg tcgagggcga aaccaacgat gccatcgagc
     4081 agctcgtcga aaagatggaa gacggcgcct ggtcgacagc gagcctcggc gcgcgttatg
     4141 acaagatgct cgatcagatc atcgcgttcc gcgcgcatgt ccgctcggcc gagcacagtt
     4201 tcaagctggg ccaggacgaa agcgccgggg gctttgccga gatcgttgcg ggccacagcg
     4261 accggacgct cgcggcgtgg atggaaggac aggcaaaaag gtgaagctct tcttcgacga
     4321 tcgccagatg gcccacgcgc cgtcgcgcga gctgcacaat ggcgactggg tgccctatgc
     4381 cgaaaatatc gagcgcccgc gttcgatcgt cgaagccttc accgactggc gcccggtgcg
     4441 cgatttcggc atggccccgc tgctcgccgt gcacgacgcc gattatctcg ccttcctcga
     4501 acgcgcgcat ggcgactgga tcgcggcagg ccgcaatggc gatgcgatcg gctacacttt
     4561 cccgatccgc cgccgccgcc cgctcgactt cgggcgcatc gatgccgatc tcggcgccta
     4621 cagctatgat gcgggaacgc cgatcgcgtc gggaacgtgg gaatcggcct attggtcggc
     4681 gcaatgcgcg ctgtcggcgc tcgacgagct ggcctggggc gatgcacgcc acgccttcgc
     4741 gctctgccgc ccgccgggcc atcacgccgg ccgcgactat atgggcggct attgctacct
     4801 caacaacgcc gcgatcgccg ggc
//
LOCUS       DATMSU010000034         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_86083, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000034 DATMSU010000000
VERSION     DATMSU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86083"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..913
                     /locus_tag="VN034_00810"
     CDS             455..913
                     /locus_tag="VN034_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59165.1"
                     /translation="MYAFVDRPVESLCNSGRFLLWAMRGWVSAAERGRCPPQMLHRGF
                     TAVTAADALPDFHVAMALLGGDAVETLVLAPLPCLQISEDEAILLGLWRDISLGNVAN
                     AHATLALLAEGDSVGPIAKAMGAAIDRLLAAGFDMPALAADTMTHQESPK"
     gene            913..1728
                     /locus_tag="VN034_00815"
     CDS             913..1728
                     /locus_tag="VN034_00815"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HWV59166.1"
                     /translation="MSDRIAEAAKLAPSASLTVEEVRSVRHWNEHLFSFTITRPPSFR
                     FRSGEFVMIGLPGEGRPLLRAYSIASPAYADELEFLSIKVPDGPLTSRLQKIQPGDPV
                     YLGRKPTGTLVADALTPGRRLFMLSTGTGLAPFLSLARDPDIYDRFNQIVIVHCVRQV
                     SDLAFREELESQLAGDPLVQDQALLQFHYLPTVTRQPFRTTGRIDALIADGSLFGHPL
                     TGPTAFDPATDRVMMCGSMAMIRDLQARFEELGFQEGSNAAPGDFVIERAFVG"
     gene            1852..2667
                     /locus_tag="VN034_00820"
     CDS             1852..2667
                     /locus_tag="VN034_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HWV59167.1"
                     /translation="MADAILHLAINFAGLLAVILLLWGVAVMIRDVSFIDAFWAFGMV
                     LLTWGTAWQVGIGAPHAKLLVGLVTLWGLRLAIHLIIRWARDGEDPRYRKMLANRIEK
                     RKWSWAKTALLTVFLTQAPLLFVTGLPAQIGIWASASAPQGIGLIGWLGAAAALAGTA
                     FESVGDAQLEAFRKNPANGGRVLDTGLWRYTRHPNYFGDALTWWGIWLVVLDLGWQPA
                     LASVIGPVFLTFTLVKWSGKALLEKGLHKTRPAYADYVKRTSGFLPWPPKTKG"
BASE COUNT          451 a          937 c          911 g          501 t
ORIGIN      
        1 agcgcggctg gaagaccgcc gccgcggcgg gcctcggcat cggctcggcg gcggtgatcg
       61 ccgcgctgct ctatgtgaac cgcgacaaga tcaaataagg tcgatctcag ggggcgggcc
      121 ccagaggggc taccgctcgg cggccgttcc gcagcgaatg gcggcgttgg ggtagggaac
      181 tgtcgttcag atcctcccct ttgcggagca atggggaggt ggcagcccgc agggctgacg
      241 gaggggttga aacctctcgt cgggacgcac cgtcccgacc cctccaccac gcccttcggg
      301 cgcggtcccc ctccccgttc cggggaggat ccttgaacgt ttcaaccggc cgttcctagc
      361 atttcgcgat cgaacataat tataaattgc aaatgactcg caataacata taagccccgc
      421 cgacgccgca tcctgcgcgt tggaaaggga atcgatgtac gccttcgtcg accggccggt
      481 ggaaagcctg tgcaacagcg ggcgtttcct gctctgggcg atgcggggat gggtctccgc
      541 ggccgagcgc ggacggtgcc cgccgcagat gctgcaccgc ggatttaccg ccgtgactgc
      601 ggcggacgcg ttgcccgatt ttcatgtcgc gatggcgctg ctcggcggcg acgcggtcga
      661 aacgctcgtc ctcgcgccgt tgccgtgcct ccagatttca gaggatgaag caattctgct
      721 tggactctgg cgcgatattt cgcttgggaa cgtcgcaaat gcccacgcga cgctggcctt
      781 gctggccgag ggagacagcg tcggcccgat cgcaaaggcg atgggcgccg cgatcgaccg
      841 gttgctcgcg gcgggtttcg atatgccggc cctcgcggcc gacaccatga cgcatcagga
      901 aagtccaaag tgatgagtga ccgtattgcc gaagccgcca agctggcccc ctccgcctcg
      961 ctgaccgtcg aggaggtgcg ctcggtgcgc cactggaacg agcatctgtt cagcttcacg
     1021 atcacccgtc cgccgagctt ccgtttccgc tcgggcgagt tcgtgatgat cggcctgccg
     1081 ggcgaaggcc gccctttgct gcgtgcctat tcgatcgcca gccccgccta tgccgacgaa
     1141 ctcgaattcc tctcgatcaa ggtgcccgac ggtccgctga cctcgcggct gcaaaagatc
     1201 cagccgggcg atcccgtcta tcttggccgc aagccgaccg gcacgctcgt cgccgacgcg
     1261 ctgacccccg gccggcggct gttcatgctc tcgaccggga ccgggctcgc gcccttcctc
     1321 agcctggcgc gcgaccccga catctatgac cggttcaacc agatcgtcat cgtccattgc
     1381 gtgcggcagg tcagcgacct cgccttccgc gaagagctcg aaagccagct cgccggcgat
     1441 ccgctggtgc aggatcaggc gctgctccag ttccattatc tgccgacggt gacgcgccag
     1501 cccttccgca ccaccggccg catcgacgcg ctgatcgccg acggttcgct cttcggccac
     1561 ccgctgaccg gtccgaccgc cttcgaccct gcgaccgacc gcgtcatgat gtgcggcagc
     1621 atggcgatga tccgcgacct tcaggcgcgc ttcgaggaac tgggtttcca ggaaggatcg
     1681 aacgcggcgc ccggcgattt cgtgatcgag cgcgccttcg tcggctaagg cctaatggcc
     1741 gccggcggac tgctggcggc ctgaaacccc gaaaagattt cgccatcgca aacgtgcggt
     1801 gcgcgcgatc gatgatatag tcggcgccga tgacgaaccg gacaggcgga catggctgac
     1861 gcaatcctgc atctcgcgat caatttcgcg gggctgctgg cggtgatcct gcttctgtgg
     1921 ggcgtcgcgg tgatgatccg cgacgtctcc ttcatcgatg ccttctgggc gttcggcatg
     1981 gtgctgctca cttggggaac ggcgtggcag gtcggtatcg gggcgccgca tgcgaagctg
     2041 ctcgtcggtc tcgtgacgct atgggggctc aggctggcga tccacctcat aatccgctgg
     2101 gcgcgcgacg gcgaggatcc gcgctaccgg aaaatgctcg cgaacaggat cgagaagcgg
     2161 aagtggagct gggcgaagac cgcgctgctc accgtcttcc tgacgcaggc gcccctgctg
     2221 ttcgtcaccg gcctgcccgc gcagatcggc atctgggcga gcgcgagcgc cccgcaaggc
     2281 atcggcctta tcggatggct tggcgccgca gcggcgctcg ccggcaccgc gttcgaaagc
     2341 gtcggcgacg cccagctcga agcgtttcgc aaaaatccgg cgaacggggg cagggtgctc
     2401 gacaccggcc tgtggcgcta tacgcgccat cccaattatt tcggcgacgc gctgacttgg
     2461 tggggcatct ggctcgtcgt cctcgacctc ggttggcagc ccgcgctcgc aagcgtcatc
     2521 gggccggtct tcctgacctt caccctcgtc aaatggtcgg gcaaggcctt gctcgaaaag
     2581 gggctgcaca agacgcggcc cgcttatgcc gactatgtaa agcgcacctc gggtttcctt
     2641 ccatggccgc caaagaccaa gggctaggca gcgaggaaag ccccgccgac gtctcggcgc
     2701 tcggcgacgc gccgcgcatc cgcgcgatcc tgatcgaggc ggcgcgcgcg gggcgcagcg
     2761 tcagctactc cgaactgctc ggcgatctgg gtttccgctt
//
LOCUS       DATMSU010000035        34156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_67186, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000035 DATMSU010000000
VERSION     DATMSU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 34156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34156
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67186"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VN034_00825"
     CDS             complement(<1..612)
                     /locus_tag="VN034_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59168.1"
                     /translation="MAEEAPLAPDDSTPAKPRSWPITVLRWVGLTLLGLVLLFALFLI
                     GLNSDAGRRFVVTQIEKYEFENGMKIGIGRLDGSLYGQMIVRDFTLSDPSGVFLRSPE
                     LRVDWRPIRYFANHIDVRSATAQTLVMEKVPAFKAVPDTGEPLLPDLDIDVGLLRVDR
                     FVFEPGVAGERQVATLSGKVAIADRRAQVSANAETISGGGGQGD"
     gene            complement(612..2900)
                     /locus_tag="VN034_00830"
     CDS             complement(612..2900)
                     /locus_tag="VN034_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWV59169.1"
                     /translation="MRPLLIGATSLIGLAWAGAAQAQDVAPPKVVPTLDTDSAEIAAP
                     SPPPPEAQLPVVEPIIEDDEFEKSIPPIRAEDDAELDRPLESIADFEKRQAAEAAKAK
                     AGTGDSEEGGAAQAQPPQVDGVPVPALADGDAVEAIGDAPISDSELAAPLPPLENFDV
                     EPVQFAEPEDGSDNVEIAYTVQVNGLSEADDSTDVDLADLFGDLSALYDGDGKADNAA
                     MIRARLSADGELMQRILASEGYYDAIVDTRIDRGPREEGQERRRRSTTAVIDVKPGKR
                     YTLADIIINAEPTVPPNLIADNFPLSVGEPIVAQRIQGAEAAIALKLPQEGYPFAKVG
                     QRDILLDGATGDGVYTLPVDIGKRSRFGGIETTGDLAFDAEHVEVLARFKRGDLYDSR
                     MVDDLRQALVATGLFATVAAEPQPTGESAGDDTEYVTMLVTQQEGPPRTLAASAGYGT
                     GQGIRVEGSWTHRNLLPPEGALTFRGVLGTQEQGIGATLRRSNAGRRDRTFELVTEVT
                     RNTYDAFNAITGRVGARVSYDSTPIWQKKFTYAYGVELIATREDDYDFATGDRSYDFY
                     TILGLTGQVGMDRTDSLLDPTKGFRITSLIQPEGSLAGRFSPYARLRTDISGYYPVTD
                     SIVLAGRVRVGSIVGAARERLAPSRRFYAGGGGSVRGFGFQELGPKDPNNDPIGGRSV
                     NEMAFEARYRFGNFGVVGFVDAGQVYRSSAPTFQNLRFGAGIGARYYTNFGPMRLDVA
                     TPIGRKPGEARVSVYVSIGQAF"
     gene            complement(3085..4278)
                     /locus_tag="VN034_00835"
     CDS             complement(3085..4278)
                     /locus_tag="VN034_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWV59170.1"
                     /translation="MAPASVSPVKIAVVGTGYVGISNAILLAQHNEVVALDIDARKVE
                     QLNAKQSPIADPEIEDYLANRPLNLVATTDRDAAYAGADFVIVATPTDYDPDTNYFNT
                     STVEKVIADALVMAPDSLIIVKSTVPVGFTDRMRGELGSDAIVFSPEFLREGRALYDN
                     LHPSRIIVGNTHPRAADFARLLLEGSLKPDAAILQTGNTEAEAIKLFANTYLAMRVAF
                     FNELDTYAAAHGVDSRQVIEGVCLDPRIGSFYNNPSFGYGGYCLPKDTKQMLANYKDV
                     PQNLIQAIVSSNTTRKDFVAAEVIKRGPRVVGIHRLAMKAGSDNFRASSILGVMKRVK
                     AKGIEVIVYEPLIEEDQLFNSRVIRDLAAFKAEADVIIANRVTDDLADVADKVYSRDL
                     FGADS"
     gene            complement(4348..5811)
                     /locus_tag="VN034_00840"
     CDS             complement(4348..5811)
                     /locus_tag="VN034_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HWV59171.1"
                     /translation="MDFAFENSFHDSMEGFYAPAEAAKPSAPELLLFNHALAERLGID
                     VAGATDDQLARVFSGEETPEGANPLALAYAGHQFGHFSPQLGDGRALLLGEIVAPDGA
                     RFDVQLKGSGPTAFSRNGDGKAAIGPVLREFLVSEAMAAMGVPTTRALAAVATGDRVQ
                     RERAHPGAVLTRVASSHIRVGTFQFFAAHFGAEHVTQLSDYGIRRHFPDLAGADNPHL
                     ALLDRVTGLQCELIAHWLGIGFIHGVMNTDNVAISGETIDYGPCAFMDRFAVGTVFSS
                     IDAGGRYAYGRQPQIMHWNMARFAEALLPAIHRVSPEDVDAAKAIVDAIPGRFRASWH
                     AMARKKLGLSGEGADDSELIDSLFDELETHGVDFTSFFRALAMLLRGDGAMLESLLPN
                     ADAMAPWIAAWWDRIAHDSAGPIEHADAMDAVNPLYIPRNHLVEEALEAAEAGDLAPW
                     LQLLGVVRHPYEVRPGLDRFTLPAPADAGPYKTFCGT"
     gene            complement(5986..7179)
                     /locus_tag="VN034_00845"
     CDS             complement(5986..7179)
                     /locus_tag="VN034_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HWV59172.1"
                     /translation="MPIFVYIRGDLPFLMDNPAAINSAAAATICTALALIAIRKFGAF
                     PGTIAASYVFPSLAASYGIAMTVFLLARAPYSGALLTLTFIASVVVRFAIGAIERRRE
                     GIQYYLVPGGSIDRIRDDLDVASITLKTPVLPSEADAIIVADLHHDHQPEWERAFAIA
                     ALNGIGVYHYKQVWEASTGKVRIEHLSENSLGSLIPSNSYAKVKRIIDIFLSLAALPF
                     LIPLMLITALAIRLDSPGSVFFRQERVGYRARPFRVLKFRTMRPRSNIADADRVDDAI
                     TKDDDVRITRIGRFLRQTRIDELPQILNILWGDMSWIGPRPEARPLSEWYEREIPFYS
                     YRHIVRPGITGWAQVNQGHVAGVSEVHDKLRYDFFYIKNFSMWIDLVILSKTILVVIR
                     GFGAK"
     gene            7732..8778
                     /locus_tag="VN034_00850"
     CDS             7732..8778
                     /locus_tag="VN034_00850"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWV59173.1"
                     /translation="METDRNEPRTPARRVLITGTAGFIGFHLATLLLAEGFEVHGYDA
                     MTDYYDVNLKHRRHQTLLQHPNFHATEGLLEDDEKLWRTAEAFAPDIIVHLAAQAGVR
                     YSLDNPRAYIESNVVGTFNLMEAARKLEVEHLLMASTSSVYGANDEMPFSEMEKADTQ
                     LTIYAATKKANESMGHSYAHLWNLPTTMFRFFTVYGPWGRPDLALYKFVDAILDNRPI
                     DIYNHGEMYRDFTHVTDLVRAIRLLIDVPPVRPASKDDIEEGDSLSPVAPFRVVNIGN
                     SDKVRLLDFVDAIEAELGIKAMRNYMPMQMGDVPATWADASLLQRLTGYRPQTDFRDG
                     IAQFVAWFREYYQK"
     gene            8804..9916
                     /locus_tag="VN034_00855"
     CDS             8804..9916
                     /locus_tag="VN034_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV59174.1"
                     /translation="MHIVLTGNTTFKLANFRAGLLRRLVGAGHAVTVMAPPDDYVGTV
                     HDMGCDFVPLKMDRNGTSPVAELRLLIAIFLNLRRLRPSFVFSYTIKNNIYAGLACRI
                     LGIPFAANVTGLGPAFNRADALNRLIRLLYRLAFRRAEAVFFQNESDRDIFLAGRLVP
                     ADRIHLLPGSGVDLTRFTARPFPASRETRFLLVARMLWDKGVGLFADAAEQVRRTHPE
                     ARFQLLGPLDPDSKTGIPQGRIDQWVEDGRLDYLGAATDVRPAFEQAHCIVLPSYYRE
                     GTPRTLLEAGATGRPVITSDMPGCRDPVIPGISGTLVRPRDSQDLARACIAFLDKSRD
                     EQAGMGRAARKKMEQDYDERIVIDAYMSLLSKAGLA"
     gene            10094..11518
                     /locus_tag="VN034_00860"
     CDS             10094..11518
                     /locus_tag="VN034_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWV59175.1"
                     /translation="MPDFRGPSRSAACCHDASSAAISEPRRSHPAGPVPLTPSHPRRR
                     IGSLVQRAKSSVLARNLAAVTSSAAVAQAIVFAFSPLITRIYGPEAFGAQGVFLSLIS
                     IFTPIIALRYPMAIVIVRRDREALYLARLSLRIALTLAGAMLLILFLFRDPISVALGA
                     DILGNLIYFLPLALFCVALQDVASFQAARQNQFSLVSRVTIIQAFLINSARVLGGLLM
                     PVAATLVAITTIAPSVLALQLWHKIRGNLPRAPRLDRRRISTLLRRHREFPLYQVPTD
                     VLNAASQSAPVILLAALFSPAAAGLYALTRSVFNLPLNIITMAMSNVLYARFAELARE
                     RRPIAPLFIKMTLLLLLPAPAIIGVAYWAPAIFSFMFGSEWREAGHYAQWMSIWIVFG
                     IANLPAIRIAPIIQGQHRLFVANILILIARVSAILAGYFYYNDARTVVILFSLVSALS
                     NIAIILDFILPTRKFDRVNCEKRS"
     gene            complement(11601..12470)
                     /locus_tag="VN034_00865"
     CDS             complement(11601..12470)
                     /locus_tag="VN034_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59176.1"
                     /translation="MHNDAYAALFARQLIASLEEMGIHVFPDLNTCWHYDDKVGQKYL
                     LEAIGAPLVPTHIFYDRAEALAWAETASFPKVWKLRGGAGSQNVRLVRTREQARRHIN
                     RSFGRGWGHDRFAALKERIWHLRRDRSWASFLAIARGVGRAIVPHAKHRHGGVERNYA
                     YFQDFVPGNDFDIRVVTIGNRAFAIKRHTRDGDFRASGSGRIAYDMREIPVDCIALSF
                     KISDRLAAQSMAYDYVFRDGEILLVEISYAFSVAGYRDCPGFWDRKLNWHEDIVRPEA
                     YMIEDVLTAIGHG"
     gene            12840..13976
                     /locus_tag="VN034_00870"
     CDS             12840..13976
                     /locus_tag="VN034_00870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV59177.1"
                     /translation="MSHLAIILPSLRGGGAEGVMMTLANAFAAQGHRVDLVLASAQGP
                     FLKEVAPGVRVIDLKSKRVTGALLPLISYFRRERPHVILSAPCHVNVVALLARAISRI
                     KARLVVSERNSMTQGLGSRSDRILLWLMRRLYPSADMVVCVSRGVEEELATLAGVPRH
                     KLRTIYNPINFEKIREGMNAPLDHPWLKQAGVPVLLAVGRLAPQKDYPTLLRGFARLR
                     QDRRARLVILGEGQERPELEKLCRDLNIATDVEFAGFQPNPFAWMAACDLFVMSSAWE
                     GLPNALLQAIACGAPVVSTDCRTGPDEILEHGKWGRLVPVGDAPGLAEAMLDALDDRS
                     PPDVQHRAEAFKIEQVVADYAEVLGLGQHNGPPMVSMPQGLFAT"
     gene            13973..14980
                     /locus_tag="VN034_00875"
     CDS             13973..14980
                     /locus_tag="VN034_00875"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HWV59178.1"
                     /translation="MTPSPDAAFARSPALPPRPTTTPIVATLFSLVFALAYANYLTNL
                     PLDAFQDRLNYFVYASSSEAILNRYASSNLSLLSNEPLWIYLNIFLGNFLYRDDIVKT
                     IIFVPAFLTAFLSLRNNSNSLFWVIIMLLVPQVIKNNIIHLRQGVAVAVFLAGYYSDR
                     RILKILLMGMAPLIHSSFFIILMIWGITYATGIIRLSVPLRVIFFTACYAIVAVVLLV
                     VASSLGARQALMYESIDPNVSGFGFAFWSVVLILFLSAGTDFVRRFAFPISILTFYLV
                     TYFAVPFAARVLESGLLIILGAGLALNDERKLLFLTLLTGFTVLSYVERLGQPWLGWG
                     V"
     gene            14959..16158
                     /locus_tag="VN034_00880"
     CDS             14959..16158
                     /locus_tag="VN034_00880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV59179.1"
                     /translation="MARLGRVTIPMAPKKVLHIITGLQDGGAEAILYRLCRRDVDDVH
                     HVVSLTGPGKYGPLLEAAGVKVTCLDMPRGQVKPKALIGLWQLIRRIRPDAIQTFMYH
                     ADLVGGVVGRLAGVRNISWGIHHSTLSPAENARSTILVARICARLSHFVPRKIICCAD
                     KGAEVHAALGYDAKRLRVIQNGYDLAVYRPDREAGNRFRTMLGIGNDPTIGFVARFVP
                     EKDHDNLFRALAILKRRGLQPACLLAGAGMDRQNAELVAMLEARDLIGDVVLLGQQDD
                     IPALMNGLDLHVMSSISEGFPNVLAEAMACGTPCVSTDVGDAADIVGGTGWIVPPGDP
                     DALAAAIEAAIQERSTLDWPKRKMDARHRVETQFSIEAMVRQYRDAWFGPEVSTVPST
                     TEGSHPA"
     gene            complement(16238..17476)
                     /locus_tag="VN034_00885"
     CDS             complement(16238..17476)
                     /locus_tag="VN034_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59180.1"
                     /translation="MSASRIFLWLAGTALAILVGMASSVIVNQVKRPATSYALGVGPD
                     GTAQGNLAFATYAARQQKDPATAVSAEERALAAEAYQREPLSSAALGILIASMPRDGE
                     EARVRQALLDIGGKLTRRSSLITSASIEAAARGGDEEAFFHWLSRATLTNESLRAAYI
                     QAMAQATARPGAEAILVNVLGPKPTWSDRYWNAVVQVPQSLVTAAKLRSLVARPPWRQ
                     TEISEADRALALRLAGAGHFDAVRELAVGLGQARASQRTGNVIVNAQFDRPSSLPPID
                     WQLATSGNLGASINAQSKALIISAISGARGYAARQLVYAEPGAYVLDWALSSNAPLAP
                     DTLEVRLACADAGPAAGAHVVPLAVGRHQQSISLASEPCRWFWLEINVKVADAGAGFD
                     AQIEHLILRRESPQDDGASS"
     gene            complement(17510..19699)
                     /locus_tag="VN034_00890"
     CDS             complement(17510..19699)
                     /locus_tag="VN034_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWV59181.1"
                     /translation="MNQVSDGQGMMTSVDPAAPRYGEAYGAAAPSAFPDIEKIFASIL
                     THRNWVVGAIVSALLLGLLATFLATPEYTSTARIEILPDAPVDTSVENDRERSTVNEL
                     AFYNTQYSLLKSQSLAERVVRAGNLTADKDFIEAFDLDVSEAGMTSAERRGLSDSAAK
                     ALLEHLDVTPVKASSLVDISFSTPSPRLSAKLAELWVRQFVQSTIDRRFAATSDARRY
                     LEARLETLRQNLETSERALINYAVNKGIVTLSSHTDSSGRTRTQTLVGSDIAQISAAL
                     SKAREDRIAAQSELANTMTGSAQINAPTIGVLRQKRAELQADLAQKLTVFADDYPSVV
                     STRAEIANLDASIRAETRRSADSNRQAYDAAVKREADLQTELDNLTGRYNSQQRESIE
                     MAILQREVDSNRQLYEGLLQRYKEIGVAGVGTNNVSMVDRAKPAEKPSSPSLLINMAV
                     ALFAGFAMAAGIVFLLEKMDSSIRDPQDVLPRFGLPLLGAIPEVIGRSVADAITDKKS
                     AAYEAYLSLMTNLSFLTEHGAPRSIMLTSSRPQEGKSNSSLCLGTVLAATGKSVILVD
                     ADVRNPSLNRYLEIPNQRGLSHYLSGDEDLDGMIAELPKFGFSIITAGKMPPNAAELL
                     GSERLGTLIATLLDRYDHVLIDSPPLLGLADAPLIARRVEGVLFTIEANSTKNRVIAT
                     ALNRLRMSGAKLFGAIVTKVGARNQVYGYGYGYGYGFSYGASKNDGE"
     gene            complement(19717..20457)
                     /locus_tag="VN034_00895"
     CDS             complement(19717..20457)
                     /locus_tag="VN034_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWV59182.1"
                     /translation="MNKILLCSVTLALLAGCAKSAPPVASAPGTPIIPLSALPEPEAS
                     DVVRSTRLAFIGPFTELRIEVFGVPEMQRDIMTDGEGNFSFPLLGNVAAAGKTPAALA
                     EELRGQLAGKYIRDPQVTVNFRAPTSATALAFQSMAVSVDGEVKNSGSYPVAGRMTLM
                     RAVALAGGVTEYAKLEDVVVFRNVGQKQYVGIYNLEAIRRGNYSDPEIFPNDVVIVGD
                     SPQRRQFRDILAATPALLSPLILLAREF"
     gene            complement(20525..21865)
                     /locus_tag="VN034_00900"
     CDS             complement(20525..21865)
                     /locus_tag="VN034_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWV59183.1"
                     /translation="MFLTGGGSRSDIASLPLLRGGAVLFACWAMVGLQRADWRRVRVP
                     LILLLALTTWTAIQLIPLSPSIWHNLPGRELIVAADRLLGNSDLWRPITLTPSETWNS
                     LLAMTVPLAALMVAARLGPEDMHRILLALLAIAIVNAVLGLLQIAWGAGSPAYLYRIT
                     NTGSMVGLFANRNHHGVFQACAILFAATLLRDELMRRQQRPAIQAGLAGTAVLCTIMT
                     MLIGSRAGLAAGAVAFVLSYAMLLPAWRGRPGRDDHRRSSKSRASWMANWMLIVPPLL
                     VAGLLAAILLLADRSTSLSRVAENRVAEDLRVLAWPTIQQMIETYWVTGAGMGSFPAV
                     YRIFESDALLQPAYFNRAHNDWVEILITGGLPSALIIVGAGVWFLRAAVRGGLRNLIK
                     GYRGDYRLPILSMVGLFAAASVVDYPLRTPSLQAMTLILIVLFACPRTAALQRE"
     gene            complement(22081..22869)
                     /locus_tag="VN034_00905"
     CDS             complement(22081..22869)
                     /locus_tag="VN034_00905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWV59184.1"
                     /translation="MLWKRKTKSVPPARGGHAIPEGQRVYAIGDIHGRDDLFAQMIDT
                     IRADNAKRDPAGVTLILLGDLVDRGPDSAGVVDRAIRLKDEFPDTRLLIGNHEECFLA
                     ALTGDVQRLRYFMRIGGDATIRSYWRDDASLAEASFEEVAAHLPTLVPAEHVDFLGRG
                     EDVIEIGDYAFVHAGVRPGIPLEKQQLSDLRWIRDEFLDDLGDHGKMIVHGHSITSEP
                     DEQSNRIGIDVGAFRSGTLAAIGLEQDRRWFILTREAEAQANAA"
     gene            23125..24240
                     /gene="wecB"
                     /locus_tag="VN034_00910"
     CDS             23125..24240
                     /gene="wecB"
                     /locus_tag="VN034_00910"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWV59185.1"
                     /translation="MKIMTVFGTRPEAIKMFPVVHALREQAGMDVRVCVTAQHREMLD
                     QVLEIARITPDIDLDVMTPNQSLDALLARLVTGLGETFDREKPDRILVHGDTLTTMAG
                     TLAAYFRKIPVGHVEAGLRSGNIYHPWPEEVNRKVAGAVADLHFAPTETAAAALRAEN
                     VAADRIHVTGNTVIDALLATKARIDEDPALAAGLDALAARFSGKRIIAVTSHRRENFG
                     EGMKAIADAIAGIAARDDVAVVFPVHPNPHVRSAMEPILGDLSNVALIDPLDYPHFVR
                     LLGMSTLVLTDSGGVQEEAPSLGKPVLVMRETTERPEGIAAGTARLVGTDKNRIVSEI
                     FSLLDDEGAYNAMARAHNPFGDGRAAERIAEIVARAH"
     gene            24230..25525
                     /gene="wecC"
                     /locus_tag="VN034_00915"
     CDS             24230..25525
                     /gene="wecC"
                     /locus_tag="VN034_00915"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HWV59186.1"
                     /translation="MPIDSEQTVTVLGLGYIGLPTAALIARSGNRVTGVDVSQHVVDT
                     VNNGKVHIEEVDLDGLVQGVVSRGALIASTEVAPADVFVIAVPTPHDEEHRPDIGHVL
                     AAARAIAPKLQAGNLVILESTSPVGTTEKVAALLAELRPDLKVPGACSGAADIFVAYC
                     PERVLPGRILVELVDNDRCIGGITPRCARRAMTFYRQFVRGACVTTSARAAEMVKLVE
                     NSYRDVNIAFANELSMMAEHMGVDVWEVIRLANRHPRVNILQPGPGVGGHCIAVDPWF
                     LVHGAPDHSRLIRTAREVNLAKTAHVIGAAEMLVSQHPKARVACLGLAFKPNIDDFRE
                     SPAVEVAAALARRFGKQIEIVEPYARGLPMEFAGTGAELIDLDSALAGCDLLIVLVDH
                     DLFKSIPIEERGDKIVYDTRGLWLDQPNGTAGNRLHRAA"
     gene            25571..26377
                     /locus_tag="VN034_00920"
     CDS             25571..26377
                     /locus_tag="VN034_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HWV59187.1"
                     /translation="MLTRLETIVVEKPWGRTDIPADFGDLGGRRIGEIWFANPAGDDA
                     PIMVKFLFTSERLSIQVHPDDKAARDAGFPRGKEECWLILNAQPGAELGVGLNRETTR
                     ETLHDAALDGSIVDMIDWRPSKENDFVYNRAGTIHAIGGGLTVVEVQQNVDCTYRLYD
                     YGRPRELHLDEGLEVSNPGPVHDPRDTVVDPAANRMLVSGPHFYLAQLAAPIDPAIVE
                     QATGELTFVPLSAGCRVAGEAIALGEAVLLTDSTGIEIDEGARALLTWPA"
     gene            26564..27130
                     /gene="hslV"
                     /locus_tag="VN034_00925"
     CDS             26564..27130
                     /gene="hslV"
                     /locus_tag="VN034_00925"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HWV59188.1"
                     /translation="MTNPHAQSAAPWHGTTILSARSADKVVIIGDGQVSMGQTVMKPN
                     ARKVRRLHDGSVIGGFAGATADAFTLFERLEAKLERHNGQLLRAAVELAKDWRTDKYL
                     RNLEAMMIVADREVTLVITGNGDVLEPKGGIAAIGSGGNYALSAARALADYEKDPEVL
                     TRKAMEVAADICVYTNDQFTSETIDIKG"
     gene            27250..28233
                     /locus_tag="VN034_00930"
     CDS             27250..28233
                     /locus_tag="VN034_00930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV59189.1"
                     /translation="MPVKASPDRVIRTLAGLRPYRPGGFVVRADTLGAKRLVHNYGHG
                     GSGITMSWGTAKLAVEMGAPGHNGPVAVIGAGIVGLSTARLLQEAGFAVTVYAKALPP
                     DTTSNIAGGQFHPFGLFKSDAVTPEWRRQFTAALDYSWRRFQIMVGDDYGVRWLTTYS
                     EGTPEEPKLPTFPPAARALRPGGHPFPVERLWQYDTMYVETGRYLRQMMRDIEGAGGR
                     IAVRGFASPAEIASLPETLVFNCTGLGARELFDDMELIPIRGQLAILIPQPEVRYAFT
                     GEAGYMFPRPDGIVIGGSYERNIWEAEPQPERIARIVAAHRQLFAGFRCPA"
     gene            28298..29596
                     /gene="hslU"
                     /locus_tag="VN034_00935"
     CDS             28298..29596
                     /gene="hslU"
                     /locus_tag="VN034_00935"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HWV59190.1"
                     /translation="MNKDLTPKAIVAALDTHIIGQTAAKRAVAVALRNRWRRQQLSPD
                     LRDEVSPKNILMIGPTGCGKTEISRRLAKLADAPFIKVEATKFTEVGYVGRDVEQIAR
                     DLVEEAVRLEKDRRREAVRAAAEEAAMERLLDALTGKGASEATRQSFRQRIREGHLDD
                     SEIEIEVADAAMSFELPGQPGQMSMINLSDMLGKAMGGPPKKRRKLKVIDAATRLIEE
                     EQDKRLDQDDVARVALADAEANGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEG
                     TTVATKYGPMKTDHILFIASGAFHVAKPSDLLPELQGRLPIRVELGALSEEDFVRILS
                     ETKAGLPEQYAALLGTEGVTLNFTDDAIARVAKLAAEVNEKVENIGARRLQTIMERLV
                     EEISFTAEDAPGTTLDIDAAYVDSQLADVVGDTDLSKYVL"
     gene            complement(29726..31291)
                     /locus_tag="VN034_00940"
     CDS             complement(29726..31291)
                     /locus_tag="VN034_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59191.1"
                     /translation="MAQILIDDGRNLESYIDDQGAYNAAIAGVSASASATANATALQA
                     AVDHVTSNGGLELQLPPGVIEIDGDIVVNPFIEDLSGSNHWNQITIRGIGAGRNGTWL
                     KFTSGSLRVRSPAHMIRDLRVSSADADGIVLEPTGGSPNRYPVRAMMSNVRVENCAGS
                     GIVLTDMWEYLMIGVFARYNANFGIEGREGIEFDLGCNALTILGGQMQGNGTIGGSPI
                     GSGGSAKSSGGGIRVGSGYQVNLYGVTLEGNVGDGLVLAEEVRGLLVDGVYFEKNGRH
                     PEDRDIRNDQPGSSPNGPNSIIIRNSNFTPQQYNGTIAERAIDLYDVHDLRIVNPQFL
                     TSGGNGYSAPPIRIRESTVGRATGWIEGRYYGSIEAYPTLLTNDTARFGFPRKQVFST
                     NLEMTKGSSDTTFGPIFVRMPKHCGRRVDVNYMTRPDAATGTARIVRDLRRGPAGTPF
                     SNPGLDVNYDSTVESVYINTNSSHNLLPGGHLEIRVTRDQGHANDTLAASLYLQSVEV
                     VTYEGVISALETS"
     gene            31558..32427
                     /locus_tag="VN034_00945"
     CDS             31558..32427
                     /locus_tag="VN034_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWV59192.1"
                     /translation="MTDEDVAPEQGELAITRTGDRLRLAREAAGLSLADVATRTRITQ
                     RHLAAIEKSDFSELPGRTYVTGFARAYARAVDLPEAEIGAAVRQELEDDEYGSRPLYE
                     AYEPTDPARLPTARLTWTLVIVTLLLASAYGVWRFLSVEPDEALIAAQNRAADESEAP
                     QNVAAATPATKAGAAAIAANAPVVLTVVGPTEVWIGFDDATGKTENWRTLDAGETYQV
                     PPAYVEQFTLRTSIPQALKVTVGGRDVGPIGPADTLVKNISLKPADLIARAEGGTTTT
                     GRTAPGTAPRPKG"
     gene            32570..33514
                     /locus_tag="VN034_00950"
     CDS             32570..33514
                     /locus_tag="VN034_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59193.1"
                     /translation="MRQITFLGTATIALAAAMPAAAQDNNIGKRVERLEKEMRAVQRS
                     VFPGGSPTFFEGEIAPDNTPGERSRTNAPVIDLTARVDALEAQLQTLTGQTEQNAFHL
                     RELEKAFTAYKAEMDKRFADPAAVDPATGDPTATPASLAPVVKAPATANATPTKKPAG
                     KPAAKPGAPDAARLALVKKVEVPSTGNETKDAYDYGYRLWDAKLYPEAQAQLKTVAEK
                     WPKSSYASFAQNLLGRAYLDEGKPSLAAVAFYNNYKDRPSGPRAPHSLVYMGIALDKL
                     GRKADACKAFRELDEVYGDKAPQDVRTDAAAAKAKAGC"
     gene            33468..>34156
                     /gene="tilS"
                     /locus_tag="VN034_00955"
     CDS             33468..>34156
                     /gene="tilS"
                     /locus_tag="VN034_00955"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWV59194.1"
                     /translation="MSAPMLPPQKQKQAADRDVAGRLAGGIAALLGPDWHRLHYGVAV
                     SGGPDSMALLWLMTSLLPGQVWAATVDHGLRKGSDEEARMVAGFCTREHIPHSTLRPP
                     APITGSQQAAARTERYRLLEQWREANALDHIVTAHHADDQLETIAMRLNRSSGVGGLA
                     AIRAKNGLILRPLLDWRRSELVQVALENDLPFVDDPSNSDDRFDRARLRHALRSQTAL
                     DPVAAARSAAWL"
BASE COUNT         6515 a        11058 c        10258 g         6325 t
ORIGIN      
        1 atcgccctgc ccgccgccgc cgctgatcgt ttcggcattg gcgctgacct gcgcccgccg
       61 atcggcgatc gcgaccttgc cgctgagcgt cgccacctgc cgctcgcctg ccacccccgg
      121 ctcgaaaacg aagcggtcga cgcggagcag cccgacgtcg atgtcgagat cgggaagcag
      181 cggctcgccg gtatcgggca ccgctttgaa cgccgggacc ttttccatca ccagcgtctg
      241 cgcagtcgcc gacctgacat cgatatggtt cgcgaaatag cggatcggcc gccaatcgac
      301 acgaagctcg ggcgaacgca ggaaaacgcc gcttggatcg gacagcgtga aatcgcggac
      361 gatcatctgg ccatagagcg agccgtcgag ccggccgatg ccgatcttca tcccgttttc
      421 gaattcatat ttctcgatct gggtgacgac gaagcgccgt ccggcatcgc tgttgagccc
      481 gatcaggaac agcgcgaaga gcaggacgag cccgagcagc gtgagcccga cccatcgcag
      541 gaccgtgatc ggccacgacc gcggctttgc cggcgtcgag tcatcggggg cgagaggcgc
      601 ctcctcggcc atcagaaggc ctgcccgatc gagacataga cgctgacccg cgcttcgccc
      661 ggcttgcgtc cgatcggcgt cgcgacgtcg agccgcatcg ggccgaaatt ggtgtaatag
      721 cgcgcgccga tcccggcgcc gaagcgcaaa ttctggaagg tcggcgccga cgagcgatag
      781 acctgccccg catcgacgaa accgacgacg ccgaaattgc cgaagcgata gcgcgcttcg
      841 aacgccatct cgttgacgct gcggccgccg atcgggtcat tgttcgggtc cttggggcca
      901 agctcctgaa agccgaagcc gcgcaccgat ccaccgccgc cggcgtaaaa gcgccgagac
      961 ggcgccagcc gctcgcgcgc cgcgccgacg atcgatccga cgcgcacccg tcctgcgagc
     1021 acaatgctgt cggtcacggg ataatagccg ctgatatcgg tgcgcagccg cgcatagggc
     1081 gaaaagcgcc cggcgagcga gccctcgggc tggatcagac tcgtgatgcg aaagcccttg
     1141 gtcggatcga gcaggctgtc ggtccggtcc atcccgacct gtccggtcag gccgaggatc
     1201 gtgtagaagt cgtaactccg gtcccccgtc gcgaaatcat aatcatcctc gcgcgtcgcg
     1261 atgagttcga ccccataggc ataggtgaat ttcttctgcc agatcggcgt cgagtcatag
     1321 ctgacgcgcg cgccgacacg gccggtgatc gcgttgaacg cgtcataggt gttgcgcgtg
     1381 acctcggtga cgagttcgaa ggtgcggtcg cggcgtccgg cgttcgagcg gcgcagcgtc
     1441 gcgccgatcc cctgctcctg cgttccgagc acgccgcgga aggtcagcgc gccttcgggc
     1501 ggcagcaggt tgcggtgggt ccagctgccc tccacccgga taccctgacc cgtgccatag
     1561 ccggcgctgg cggccagcgt gcgcggcggc ccctcctgct gggtcaccag catcgtcaca
     1621 tattcggtat cgtcgccggc gctctcgccg gtcggctgcg gctcggcggc gacggtcgcg
     1681 aacaggcccg tggcgacgag cgcctggcgg aggtcgtcga ccatccgact gtcatagagg
     1741 tcgccgcgct tgaagcgcgc gagcacctcg acatgctcgg catcgaaggc gagatcgccg
     1801 gtggtctcga tcccgccgaa acgcgagcgt ttgccgatat cgacgggcag cgtgtagacg
     1861 ccgtcgccgg tcgcgccgtc gagcaatata tcgcgctgcc cgaccttggc gaaaggatag
     1921 ccttcctggg gcagcttgag cgcgatcgcc gcctcggcgc cctgaatgcg ctgcgcgacg
     1981 atcggctcgc cgacgctcag cggaaaattg tcggcgatca gattgggcgg aaccgtcggc
     2041 tcggcattga tgatgatgtc cgccagagtg tagcgtttgc ccggcttcac atcgatcacc
     2101 gcggtggtcg accggcgccg gcgctcctgc ccctcctcgc gcgggccgcg gtcgatgcgg
     2161 gtgtcgacga tggcgtcata atagccttcg gaggcgagga tgcgctgcat caactcgccg
     2221 tcggctgaga gacgcgcgcg gatcatcgcc gcattgtcgg ccttgccgtc gccgtcgtag
     2281 agcgccgaca gatcgccgaa caggtcggcc aagtcgacat cggtgctgtc gtcggcctcg
     2341 ctcagcccgt tgacctgtac cgtataggcg atctcgacat tgtcactgcc gtcttcgggc
     2401 tcggcgaact ggacaggctc gacgtcgaaa ttttcgagcg gcggcaacgg cgcggcgagt
     2461 tcgctgtcgc tgatcggcgc gtcgccgatc gcctcgaccg catcaccgtc ggccaaggcg
     2521 ggcaccggca ccccgtccac ttgcggtggc tgcgcttgag ctgccccccc ctcttcgctg
     2581 tcgccggtgc ccgccttggc cttcgccgct tcggccgcct ggcgcttctc gaaatcggcg
     2641 atcgattcga gcggccggtc gagttcggca tcgtcctcgg cgcggatcgg cgggatcgat
     2701 ttttcgaact cgtcatcctc gatgatcggc tcgacgacgg gcagttgcgc ttcgggcggc
     2761 ggtggcgacg gcgcggcgat ctccgccgaa tcggtgtcga gcgtcggcac gaccttcggc
     2821 ggcgcgacat cctgcgcctg cgcggcgccc gcccaggcaa gaccaatgag tgaagtcgcg
     2881 ccgatcagca gcgggcgcat ccgtgggtgc gggcgccaac atgatctgcg aaaaatttca
     2941 gattgtttca tctgctggcc accgatgcat gcttgtggct cgcgagatat ggctgtgcaa
     3001 cccgtttctc ccctaattgc aagctaactc gccgagatcg ggacagttcc cggcgatgcg
     3061 gcgagttgcg tccggggaaa acaatcagga atcggcgccg aacagatcgc ggctgtaaac
     3121 cttgtccgcg acgtcggcga gatcgtcggt cacacggttc gcgatgatga cgtcggcctc
     3181 ggccttgaag gcggcgagat cgcggatcac gcgcgagttg aagagctggt cctcctcgat
     3241 cagcggttca tagacgatca cctcgatccc cttcgccttg acgcgcttca tcacgcccag
     3301 gatgctgctg gcgcgaaaat tgtccgaccc cgccttcatc gcgaggcgat ggatgccgac
     3361 gacgcggggc ccgcgcttga tcacttcggc cgccacgaaa tccttgcgcg tcgtgttcga
     3421 cgacacgatc gcctggatca gattctgcgg cacatccttg taattggcga gcatctgctt
     3481 cgtgtccttg ggcaggcaat agccgccata gccgaaggag ggattgttgt agaaactgcc
     3541 gatccgggga tcgaggcaca cgccctcgat cacctggcgc gaatcgaccc cgtgcgcggc
     3601 ggcataagtg tcgagttcgt tgaagaaagc gacgcgcatc gcgagatagg tgttggcgaa
     3661 cagcttgatc gcctccgcct cggtattgcc cgtctgcagg atcgccgcat cgggcttcag
     3721 cgacccttcg agcagcaggc gcgcgaaatc ggccgcgcgc gggtgcgtat tgccgacgat
     3781 gatccgcgag ggatggagat tgtcgtaaag tgcgcgccct tcgcgcagga attcgggcga
     3841 aaagacgatc gcatcactgc cgagttcacc gcgcatccgg tcggtgaagc cgaccgggac
     3901 ggtcgatttg acgatgatca gcgagtcggg cgccatcacg agcgcgtcgg cgatcacctt
     3961 ctcgaccgtg ctggtgttga aataattggt gtcgggatca tagtcggtcg gcgtcgccac
     4021 gatgacgaaa tccgcgccgg cataggccgc gtcgcgatcg gtcgtcgcga cgaggttcag
     4081 cggccggttc gccagataat cctcgatctc ggggtcggcg atcggcgatt gcttcgcatt
     4141 gagctgctcg accttgcgcg catcgatgtc gagcgcgacc acctcattat gctgcgccag
     4201 caggatcgcg ttggaaatgc cgacataacc cgtgccgacg acggcgatct tgacgggcga
     4261 aacagatgct ggggccaatg acttatcctc tgagtggtgc ggcgccgtcc ctagcaactg
     4321 cgatgcaccg gctcaaccgc ggccgcgtca ggtgccgcag aaggtcttgt acggccccgc
     4381 atcggccggc gcgggaagcg tgaagcggtc gagccccggg cggacttcat aaggatggcg
     4441 cacgacaccg agcagctgga gccacggcgc gagatcgccg gcctcggccg cctcgagcgc
     4501 ctcctcgacc agatggttgc gcgggatata gagcggattc accgcatcca tcgcatcggc
     4561 atgttcgatc ggtcccgcgc tgtcgtgcgc gatcctgtcc caccacgcgg cgatccacgg
     4621 cgccatcgcg tcggcattcg gaagcagact ctcgagcatc gcgccatcgc cgcgcagcag
     4681 catcgcgagc gcgcggaaga aggaggtgaa atcgacgccg tgcgtttcga gctcgtcgaa
     4741 caggctgtcg atcaactcgc tatcgtcggc accctcgccg ctcagcccga gcttcttcct
     4801 ggccattgcg tgccagctgg cgcggaaccg gccgggtatc gcatcgacga tcgccttcgc
     4861 cgcgtcgaca tcctcgggcg acacgcggtg gatcgcgggc agcaacgcct cggcaaagcg
     4921 cgccatgttc cagtgcatga tctgcggctg gcggccatag gcatagcggc cgcccgcgtc
     4981 gatcgagctg aagacggttc ccacggcaaa gcgatccatg aaggcgcagg ggccataatc
     5041 gatcgtctcg ccgctgatcg cgacattgtc ggtgttcatc accccgtgga tgaagccgat
     5101 gccgagccag tgggcgatca gctcgcattg caggcccgtg acccggtcga gcagcgcgag
     5161 atgcgggttg tcggcgccgg cgaggtccgg gaaatggcgc ctgatgccgt aatccgacaa
     5221 ttgggtgaca tgctcggcgc cgaaatgcgc ggcgaagaac tggaaggtcc cgacgcgaat
     5281 gtggctgctg gcgacgcgcg tgaggactgc gccgggatgc gcgcgttcgc gctgcaccct
     5341 gtctccggtc gcaaccgcgg cgagcgcgcg tgtcgtcggc acgcccatcg ccgccatcgc
     5401 ctcggacacg aggaattcgc gcagcaccgg accgatcgcc gccttgccgt cgccgttgcg
     5461 cgaaaaggcg gtcggccccg atcccttgag ctggacatcg aaacgcgcgc cgtcgggcgc
     5521 gacaatctcg ccgagcagca gcgcgcgtcc gtcgccgagc tgcggcgaga aatggccgaa
     5581 ctgatgcccg gcataagcaa gcgcgagcgg gtttgcgcct tcgggcgtct cttcgcccga
     5641 aaagacacgc gcgagctgat cgtccgtcgc acccgcgaca tcgatcccga gccgctcggc
     5701 gagcgcatgg ttgaagagga gcagctccgg cgcggagggc ttggcggctt ccgcgggcgc
     5761 gtaaaagcct tccatactgt cgtgaaaact attttcgaac gcgaaatcca tggcgcctat
     5821 gtcgagcctc cgggaacatt ttgcaagaat ggcagccggc gtcatttggc ttccggccgc
     5881 ctgcagatcc cgaactttcg cgcttccggt gctcgcgtgc agatcgccgg acgcgtattg
     5941 cccgggaaat cagtcagcgc ccgctgcaca ccgccgacag atggcctatt ttgcgccgaa
     6001 accccggatc acgaccaata tcgtcttgga aagaatgacg aggtcgatcc acatcgagaa
     6061 gttcttgata tagaaaaagt catagcgaag cttgtcatgc acctcgctga ctcccgcgac
     6121 atgcccttgg ttgacctgcg cccacccggt gattccgggc cgcacgatgt gacgatagga
     6181 atagaagggg atttcccttt cataccattc ggaaagcggc cgcgcttcgg gccggggccc
     6241 gatccagctc atatcccccc acagaatatt gaggatctgc ggcaattcgt cgattcgcgt
     6301 ttgccgcaga aaacgaccga tcctggtgat ccgaacgtca tcgtccttgg tgatcgcatc
     6361 atcgacgcga tcggcatccg cgatgttcga gcgggggcgc atcgttcgaa atttgagcac
     6421 gcgaaacggg cgggcccgat atcccaccct ttcctgccgg aagaagaccg accccggcga
     6481 atcgagccgg atcgcaagcg ccgtgatcag catcaggggg atgagaaaag gcaaggcggc
     6541 cagcgagagg aaaatatcga tgatccgttt caccttcgca taggaattgc tggggatcag
     6601 cgatccgagg ctgttttccg acagatgctc aatgcgcacc ttgccggtcg aggcctccca
     6661 tacctgcttg taatgataga cgccgatgcc gttgagcgcg gcgatagcaa acgcccgttc
     6721 ccattccggc tgatgatcgt gatgcagatc ggccacgatg atcgcatcgg cctcgctcgg
     6781 tagcaccgga gttttcagcg tgatcgacgc gacatcaaga tcgtcgcgaa tacggtcgat
     6841 actgcccccc ggcaccagat agtattgaat gccttcgcgg cgacgctcga tcgccccgat
     6901 cgcaaaacgc acgacaacgc tggcgatgaa ggtcagcgtg agcagcgcgc cgctataggg
     6961 cgcacgcgcc agcaggaaca cggtcatcgc gatgccatag ctggcggcca gcgacggaaa
     7021 aacatagctc gcggcgatgg taccgggaaa ggcgccgaat ttgcgaatcg cgatcagcgc
     7081 cagcgccgtg cagatcgtcg cggcagcggc cgaattgatc gccgccggat tatccatcag
     7141 gaacggcagg tcgccgcgaa tatagacgaa tatgggcaag aataccccga acacgaggca
     7201 gaccagcaac tgcgcccgga gcgatccgaa ataattgaaa aacgagcggg gggaagccgc
     7261 aacgcggtca gtctgattca aaggtagagc tttcaactaa tattcccgcc aataacagcc
     7321 aattctccgg aaatcagaaa tgatttttat accggtcctg attccccgaa tttttccaca
     7381 gcgtcctcca acgcccgggc ccggggaccg ttgcaagacc cgtgggcccg atacatattt
     7441 tgcgcgggtc cgtcgcggat atattcttgc cttcaaggca gatcgtgcac ggccgatccc
     7501 atccgccggt cgccgatcac tgctccgagg cttggggaaa caggtcgtcg cacttcgaaa
     7561 gcctctcatg ccgcccaatc ggcgtttgct tattgcccac gctgggctaa tggcttggcc
     7621 gcatcctcaa acggaccatc gaagggcgct atcgaccagg cggaatgtta attgctatga
     7681 cgccggtccg gcgcgccggg tcgcccctcc ctatcatatt ggacgaagaa tatggaaacc
     7741 gaccggaacg aaccgcgcac gcccgcgcgc cgggttttga tcacgggcac ggcgggcttt
     7801 atcggctttc atcttgccac cttgctgctc gccgaggggt tcgaggttca cggttatgat
     7861 gcgatgaccg actattatga cgtcaattta aagcaccgcc gacaccagac cctgttgcag
     7921 cacccgaatt tccacgcgac cgagggcttg ctcgaggatg acgagaagct ctggcgtact
     7981 gccgaagcct tcgcccccga catcatcgtt catctcgccg cacaggccgg ggtccgctac
     8041 agcctcgaca atccgcgcgc ctatatcgaa agcaatgtcg tcggcacctt caacctcatg
     8101 gaggccgcgc gcaagctgga ggtcgaacat ctgctgatgg cgtccacctc gtcggtctat
     8161 ggagccaacg acgaaatgcc cttttccgag atggaaaagg ccgacacgca gctgaccatc
     8221 tatgccgcga cgaagaaggc caacgagagc atggggcatt cctatgccca tctgtggaat
     8281 ttgccgacga cgatgttccg cttttttacg gtctacggtc cgtggggacg ccccgacctt
     8341 gcgctttaca aattcgtcga tgccatcctc gataaccgcc cgatcgacat ctacaatcat
     8401 ggcgagatgt accgcgactt cacccatgtc accgacctcg tccgcgccat ccggctgctg
     8461 atcgacgtgc cgccggtgcg cccggcgtcg aaggacgaca tcgaggaagg cgacagcctt
     8521 tccccggtcg cgcccttccg ggttgtcaac atcggcaatt ccgacaaggt gcggctgctc
     8581 gatttcgtcg acgcgatcga ggccgaactg gggatcaagg ccatgcgcaa ctatatgccg
     8641 atgcagatgg gcgatgtccc cgcgacctgg gccgacgcct cgctccttca gcgcctcacc
     8701 ggctatcgtc cgcaaaccga tttccgcgac ggaatcgcgc agttcgtggc atggttccgc
     8761 gaatattacc agaagtagcg ggcaacaggc ggaaggcggc ggcgtgcata tcgtcctgac
     8821 cggcaatacg actttcaaac tcgccaattt tcgggcggga ctgttgcggc gcctggtcgg
     8881 cgccggacac gctgtaaccg tgatggcgcc gcccgatgac tatgtcggca cagtgcacga
     8941 catgggttgc gactttgtcc cgctgaaaat ggatcgcaac ggcacctcac cggttgccga
     9001 gcttcgattg ttgatcgcca ttttcctcaa cctgcggcgt cttcgcccct cgtttgtatt
     9061 cagctatacg atcaagaaca atatttacgc cggcctggcg tgccgcattc tggggatccc
     9121 cttcgcggcg aatgtcaccg gcctaggccc cgccttcaac cgcgccgacg ctttgaaccg
     9181 cctgatccgg ctcctctacc ggctggcctt ccggcgcgcc gaagcagtgt ttttccagaa
     9241 cgaaagcgac cgcgatattt tcttggccgg tcgcctcgtg ccggcggatc gaattcacct
     9301 cctgcccggc tcgggcgtgg atttgacccg tttcaccgcg cggccatttc cagcgtcgcg
     9361 cgagacgcga ttcctgctgg tcgcgcggat gttgtgggac aagggcgtgg gcttgtttgc
     9421 cgacgccgcc gagcaggtgc ggcgcacgca tcccgaggcg cgattccagc tgctcggacc
     9481 gctcgatccc gacagcaaga cggggattcc gcaaggccgg atcgaccaat gggtcgaaga
     9541 tggccggctc gattatctgg gcgcagctac cgatgtgcgc ccggcgttcg agcaggcgca
     9601 ctgcatcgtg ctgccatcct attatcgcga aggcacgccc cgcaccttgc tggaagcggg
     9661 agcgacggga cgcccggtta tcacgtccga catgccggga tgcagagatc ctgtcattcc
     9721 gggaatatcg gggacgctcg tccgaccgcg cgacagccag gatctcgcgc gcgcttgcat
     9781 cgcatttctg gacaagtcgc gcgacgagca agccggcatg ggccgtgcgg cacgaaaaaa
     9841 gatggagcag gattacgacg aaaggattgt gatcgacgcc tatatgagct tgttgagcaa
     9901 ggctgggctc gcttaaggaa gacaggagca tcgcaccgcg ctcgctcccg gccttccctt
     9961 tgaacaagag ccatgactgc cgtcgggacg gtctcgacga tcttcgcccc ccggaccgat
    10021 tgaaagacga tagagatgaa tgctgtggac gaggggttga acgacgcgaa gatcggcgct
    10081 gtcgcgcttg gccatgccgg acttccgtgg gccgtcgcgt tcagccgcct gctgtcatga
    10141 tgcctcgtcg gccgccatct ccgaaccgcg tcgatcgcac cctgccggac cggttccatt
    10201 gacgccgtcc catccccgac gacggatcgg ttccctcgtg caacgggcga agtcgtcggt
    10261 tcttgcaaga aacctcgccg cggtcacgag tagcgcggcc gtggctcaag cgatcgtttt
    10321 cgctttctcg ccattgataa cgcgcattta cggccccgaa gcctttggcg ctcagggtgt
    10381 tttcctttcg ctgatcagca tattcacgcc gatcatagcc cttcgctatc cgatggcgat
    10441 cgtcatcgtc cggcgcgaca gggaagccct atacctcgct cgactgtcgc tgcgaattgc
    10501 cctcactttg gcgggggcga tgctcctgat actctttttg ttccgggatc ctatttccgt
    10561 tgccttgggc gcggatattc tgggtaatct catctatttc ctgccgcttg ccctgttctg
    10621 cgtggcgctg caggatgtgg cctcgtttca ggcggcgcgg caaaaccaat tcagcctggt
    10681 ttccagggtg acgatcattc aggcgttcct gatcaattcc gcccgggttc ttggcggttt
    10741 gctcatgccc gttgcggcga ctctggtcgc gataacgacc atcgcaccct cggttctggc
    10801 gcttcagctg tggcataaga tacgcggcaa cctgccgcgg gcaccgcgcc ttgatcggcg
    10861 acgcatttcc accctgctac gacggcaccg tgaatttccg ctctatcagg tgcctaccga
    10921 cgtgttgaac gccgcgtcgc aatccgctcc ggtcatcctt ctcgcggccc tgttttcccc
    10981 cgccgcagcg ggtctttatg ccttgacgcg cagcgtcttc aatctcccgc tcaacatcat
    11041 caccatggcc atgagcaatg tgctctacgc ccgtttcgcc gaactggcac gcgaacgccg
    11101 gccgattgcc cccttgttta tcaagatgac attgcttctg ctgctgccag cgcctgcaat
    11161 cattggcgta gcctattggg cgccggcgat attctctttc atgttcggtt cggaatggcg
    11221 tgaagcaggg cattatgccc aatggatgtc catctggatc gttttcggga tcgcgaattt
    11281 gcccgccatc cggatcgctc cgattatcca aggacaacat cgtcttttcg tggcgaatat
    11341 cctcatattg atcgcacgcg tttcggccat tcttgccggg tatttttact acaatgacgc
    11401 gcgcaccgtc gtcattctgt tttccttggt cagcgcgctg tcgaatatcg ccattatcct
    11461 cgatttcatt ctgccgaccc ggaagttcga tcgcgtgaat tgcgaaaaaa gatcatagtg
    11521 tgaaaaatca gcgaaggttc gcgcggtgcg gcacgttacc cggttcagcc gatagggtcc
    11581 aaaccgcgac ggaaaatccc tcatccgtgg ccaatcgcgg tcaagacatc ctcgatcata
    11641 taggcttccg ggcgcacaat atcctcatgc cagttcagct tacggtccca aaaccccgga
    11701 caatcccggt atccggcaac cgaaaaggca tagctgatct cgaccagcaa aatctctcca
    11761 tctcgaaaga cataatcata agccattgat tgggccgcga gccggtccga aatcttgaac
    11821 gacaaggcga tgcagtccac ggggatttcg cgcatgtcgt aggcgatgcg cccgctcccc
    11881 gatgcgcgaa agtcgccgtc gcgggtgtga cgcttgatcg caaaggcgcg gttgccaatc
    11941 gtcaccaccc ggatgtcgaa atcattgccg ggcacgaaat cctggaaata ggcatagttg
    12001 cgctcgacac cgccatgccg gtgctttgcg tgcgggacaa tcgctcgacc gacgccccgc
    12061 gcgatggcca gaaacgacgc ccaggaccga tcgcggcgca aatgccatat ccgttccttg
    12121 agagcagcaa aacggtcgtg cccccatccg cggccaaagc tcctgttgat gtggcggcgc
    12181 gcctgctccc gcgtccggac cagacgaaca ttctgcgacc ccgcgccacc gcgcagtttc
    12241 cacaccttcg gaaaactcgc cgtttccgcc catgcgagcg cctctgctcg atcatagaat
    12301 atatgcgtcg gaaccagcgg tgccccgatc gcctcgagaa gatatttctg gccgaccttg
    12361 tcgtcataat gccagcatgt gttcagatcg gggaaaacat gaattcccat ctcttcaagc
    12421 gaggcgatca actgccgtgc gaagagcgcc gcataggcgt cgttgtgcac ccaatgccaa
    12481 agcagggcgc gacaaccgcg caactggctg acaatgtccg agcggaaaca atccacctct
    12541 ttatgtggga tttgacgggc tttacaatat tcgacccagg cctttgcgaa aggccccgat
    12601 ttcgtgtgaa tcgctatcat gttttcgaac tgtttctgat ctttcgtatc gatcgatcag
    12661 gaagccgatc aatccgcttg tgagattccg gcgcggcgat gccatgttgg gccagattgt
    12721 gcagtcgggg gatgacggca cttgtgatcc gatgtcaatc tgcaatatgg ccccagcatc
    12781 tgcctcggcg tccgccgaag cctcgcacga ccagttcgat caataggacc cctgccctta
    12841 tgagccacct cgcgatcatt ctcccctcct tgcgcggggg aggcgccgaa ggggtgatga
    12901 tgacgttggc caacgctttc gcggcacaag gccatcgggt ggatttggtt ctcgccagcg
    12961 ctcaggggcc cttcctgaag gaggtggcgc ccggcgtccg cgtgatcgat ttgaaaagca
    13021 aaagggtcac cggtgccctt ttgccgctga tttcctattt tcgtcgggaa cggccgcacg
    13081 tcattctttc tgcaccctgc cacgtgaacg tcgtggctct tttggcacgc gccatcagcc
    13141 gtataaaggc gcgtctggtc gtgagcgagc ggaacagcat gacccagggc ctgggatccc
    13201 gttccgatcg aatattgctc tggctgatgc gccggctcta tccgtccgcc gatatggtcg
    13261 tgtgcgtatc tcgcggggtc gaagaagaac ttgcgacgtt ggccggcgtt ccccggcata
    13321 agcttcgcac catatataat ccgattaact tcgagaaaat tcgcgagggc atgaacgcgc
    13381 cgctggatca tccttggttg aagcaggcgg gcgttccggt tctgctggcc gtcgggaggc
    13441 tggcaccgca aaaggattac ccgacgctat tgcgcggctt cgcacggctt cggcaggatc
    13501 ggcgggcccg gctggtaatt ttgggagagg gtcaggaacg acccgagctc gagaaacttt
    13561 gccgcgatct gaatatcgca accgatgtcg agttcgccgg ctttcaacca aatcctttcg
    13621 cgtggatggc ggcatgcgac ctgttcgtca tgtcgtccgc ctgggaaggg ctgccgaacg
    13681 ccctgttgca ggcgatagct tgcggcgcgc ccgtcgtgag caccgactgc agaaccgggc
    13741 cggacgagat attggagcat ggaaaatggg ggcgactggt gccggtcggc gacgcccccg
    13801 ggcttgcgga ggccatgctc gatgcgctgg acgatcgatc gcctcctgat gtacagcatc
    13861 gtgccgaggc attcaaaatc gaacaggtgg ttgcggatta tgccgaagtt ctcgggcttg
    13921 gacagcacaa cggcccgccg atggtaagta tgccccaggg attgtttgca acgtgacccc
    13981 ctcgcccgac gccgcgttcg cacgatcgcc tgcccttcca cccaggccga cgactacgcc
    14041 cattgtcgcg acattattct cgctggtatt tgcgttggcc tatgcaaatt acctgaccaa
    14101 cctgccgctc gatgcatttc aggacagact gaattatttt gtctacgcca gcagcagtga
    14161 ggccattcta aatcgctacg cgagcagcaa cctttcatta ctttctaatg aaccactttg
    14221 gatttacttg aatatatttc taggaaattt cctatataga gatgatattg tcaagacaat
    14281 catatttgtt ccggcattct taactgcatt tttgtcccta agaaacaata gcaacagcct
    14341 tttttgggtt attattatgt tattggtacc gcaagttata aagaataata taattcatct
    14401 tcgtcaaggc gtcgcggtcg cagtttttct agcaggatat tattcagatc gaaggatatt
    14461 aaagatcctc ctgatgggga tggcgcctct gatccattcc tctttcttca tcattctgat
    14521 gatttgggga attacatatg cgaccgggat catacgtctc tcggttcctt tgcgggtcat
    14581 cttcttcacc gcgtgctacg ccatcgtggc ggttgtcctg ctcgtcgtgg catcgagcct
    14641 gggggcgcgc caggcattga tgtatgaatc catcgacccc aacgtcagcg gcttcggttt
    14701 cgcattctgg agcgtggtcc ttattctgtt cctttcggcg ggaacggatt tcgtccgtcg
    14761 tttcgctttt cccatctcca tcctgacgtt ctacctcgtc acctatttcg ccgtgccctt
    14821 tgccgcgcgt gttctggaaa gcggcttgct gatcatcctg ggcgcggggc tggcgctcaa
    14881 cgatgaacga aagcttctgt tcttgacgct gcttacgggg ttcaccgtct tgagctacgt
    14941 cgaacgactg ggtcagccat ggctcggctg gggcgtgtaa ccatcccgat ggccccgaaa
    15001 aaggtcctcc atatcataac cgggctgcag gacggcggag cggaggcgat cctctatcgc
    15061 ctgtgccgcc gcgatgtcga tgacgttcat catgtcgtat ccctgaccgg acccggaaaa
    15121 tacgggccat tgctggaggc ggccggcgtg aaagtcacct gcctcgacat gccgcgaggc
    15181 caagtgaaac cgaaggcgct gatcgggctt tggcagctga tacgccgcat ccgtccggac
    15241 gcgatccaga cattcatgta ccatgcggac ctcgtgggcg gtgtcgtggg tcgcctcgcc
    15301 ggcgttcgca atataagctg gggcattcac cactcgacat tgtcgcctgc cgaaaacgcc
    15361 cgttcaacca ttttggtcgc gcgcatctgc gccagactct cgcacttcgt tccgcggaag
    15421 atcatctgct gcgccgacaa gggggccgag gttcacgccg cactcggcta tgatgccaaa
    15481 cggcttcggg tcatccagaa tggatatgac ctcgccgtct atcgtcccga ccgggaggcg
    15541 ggaaatcgct ttcgcacaat gctcggcata gggaacgacc cgacaatcgg tttcgtagcc
    15601 cgcttcgttc cggaaaagga tcatgacaat ctatttcgcg cactggcgat attgaagcgg
    15661 cgcggattgc agccggcctg cctgctggca ggtgcgggca tggatcgtca aaatgcggaa
    15721 ctggttgcga tgctcgaagc gcgggatctg atcggcgatg tcgttctgct cggacagcaa
    15781 gacgatattc cggccctgat gaatgggctt gaccttcatg tgatgtcttc gatcagcgaa
    15841 gggtttccga atgttctggc cgaagcgatg gcctgcggca cgccatgtgt atccaccgat
    15901 gtcggcgacg cggcggacat cgttggcggc accggatgga ttgtcccgcc gggcgatccc
    15961 gatgctttgg ccgctgccat cgaggccgcg attcaagagc ggtcgacgtt ggattggccg
    16021 aagcgcaaaa tggatgcgcg tcaccgcgtc gaaacgcaat tttccattga agcgatggtt
    16081 cggcaatatc gcgacgcctg gttcggaccg gaagtttcaa ccgttccttc gacgaccgaa
    16141 ggctcgcatc cggcatagac caagcttcgc tcgcgccgcg caaccgttct tctccgggcg
    16201 gccacatgct tgttcggcgc cgatcccctg cccgcggtta agacgacgcc ccgtcatcct
    16261 gagggctttc ccggcggagg atcaaatgct cgatctgggc atcgaaaccc gctccggcat
    16321 cagccacttt cacattgatt tcgagccaaa accatcggca cggttcggat gccagcgaaa
    16381 tggactgctg atggcgtccg acggccagcg gcaccacatg cgcacctgcg gcgggtcccg
    16441 catcggcgca ggccaaccgc acctccagcg tatccggcgc aagcggggca ttgctcgaca
    16501 gggcccagtc cagcacatat gcgcccggct ccgcataaac gagctgacgg gcggcatagc
    16561 cgcgcgcgcc cgaaatggcg gaaatgatca aggctttgct ttgcgcgttg atcgacgcgc
    16621 ccaaattacc ggacgttgcc agttgccaat cgatcggcgg cagcgaggac ggtcggtcga
    16681 actgcgcgtt gacgatgaca ttgcctgtcc gctgcgacgc gcgcgcctgc cccaacccga
    16741 ccgccagttc gcgcacggcg tcgaaatgtc cggcaccggc cagtcgcagg gcaagcgccc
    16801 ggtcggcttc gctaatctcc gtctgtcgcc aaggcgggcg cgcaacaagg ctgcgcaact
    16861 tcgcggcagt gaccaggctt tgcggaacct gcacgaccgc gttccaatat cggtcggacc
    16921 acgtcggttt cggtcccagc acattgacga gaatcgcttc ggcgcctggc cgcgcggtag
    16981 cctgcgccat tgcctggata taagcggcgc gtaagctctc gttcgtcaaa gtagcccggg
    17041 aaagccagtg gaaaaacgcc tcctcatcac cgccgcgcgc cgcggcctcg atcgacgccg
    17101 aggtaatcag cgaactgcgg cgcgtaagct ttccgccgat gtccagcagg gcttgccgga
    17161 cgcgcgcttc ctcaccgtcc ctgggcatcg aggcgatcaa tatcccgagc gccgctgacg
    17221 acaatggttc gcgctgatag gcttcggcgg cgagcgcacg ttcttcggcg gacaccgcag
    17281 tcgccgggtc cttctgctgt cgcgcggcat aggttgcgaa cgccaggttg ccctgcgccg
    17341 tgccgtcggg cccgacgcca agcgcatagg atgtggccgg ccttttcact tggttgacga
    17401 tcacgctgct cgccatgccg accagaatcg ccagcgcggt gccggcaagc cacaagaata
    17461 tccgcgaagc gctcacaatg tcaccaaatc ccacagcgga acgacaatcc tattcgccgt
    17521 cgttcttgct ggcaccatag ctgaagccat agccatagcc ataaccatag ccataaacct
    17581 gattgcgcgc gccaaccttg gtcacgatcg caccgaacag cttggctccc gacatgcgca
    17641 gccggttcaa cgccgtcgcg atcacccggt tcttggtcga gttcgcctcg atcgtgaaca
    17701 gcacgccttc aacgcggcgc gcaatcaacg gtgcgtcggc gagcccgagc agcggcgggg
    17761 aatcgatcag cacatgatca tagcggtcca gcagcgtcgc gataagcgtc ccgagccgtt
    17821 cgctacccag caattcggcc gcattgggcg gcatcttgcc cgccgtaatg atggaaaatc
    17881 cgaacttcgg aagttcggcg atcatgccgt cgagatcctc gtcgcccgac agatagtgac
    17941 tcagtccgcg ttggttcgga atttcgagat agcgattgag cgacggattg cggacgtccg
    18001 catcgacgag gatgaccgat tttccggtcg ctgccagcac cgtgccaagg caaagcgacg
    18061 aattgctctt tccctcctgc ggccgtgacg aggtcagcat gatcgatcgc ggcgcgccat
    18121 gctcggtcag gaaggacaaa ttggtcatca gcgacaaata ggcctcgtaa gcggcggatt
    18181 tcttgtccgt gatggcgtcc gcgaccgacc gaccaatcac ttcgggaatc gcccccagca
    18241 acggcaggcc gaaccgcggc aggacatctt gcggatcgcg gatcgaacta tccatcttct
    18301 ccagcaagaa aacgataccc gcggccatgg cgaaaccggc aaacagcgcc accgccatgt
    18361 tgatcaacag gctggggctc gatggctttt ccgcaggttt ggcgcggtcg accatcgaaa
    18421 cattgttcgt cccaacgccg gcgacgccga tttccttgta gcgctgcaac agcccttcgt
    18481 agagttggcg gttcgaatcg acctcgcgct gcaggatcgc catttcgatc gattcacgct
    18541 gctgactgtt atagcgaccg gtaagattgt cgagttccgt ctgcagatca gcctcgcgct
    18601 ttaccgccgc atcataggct tgacgattgc tgtccgccga ccgccgggtc tctgccctta
    18661 tgctggcgtc gagattggca atctctgcgc gagtcgacac caccgacgga taatcgtcgg
    18721 cgaagacggt gagcttttgc gccagatcgg cctggagctc ggcgcgcttc tgccgcaaca
    18781 cgccgatggt cggcgcgttt atctgcgcgc ttcccgtcat ggtgttcgca agttcggatt
    18841 gtgctgcgat tcggtcctcg cgcgctttcg acaaggcggc gctgatctgg gcaatgtccg
    18901 atcccaccag ggtttgcgtg cgcgttcgcc cgctactgtc ggtatgcgac gacagggtga
    18961 cgatcccctt gttcaccgca tagttgatca gcgcccgttc ggacgtctcc aaattttgtc
    19021 gaagcgtttc aagacgcgct tccagatatc tgcgcgcgtc cgaagtggcg gcgaagcgtc
    19081 ggtcgatggt agactggacg aattgccgga cccacaactc ggccagcttg gccgacagac
    19141 ggggcgaggg cgtcgaaaag ctgatgtcca cgaggctcga cgccttgact ggcgttacat
    19201 cgagatgctc gagcagggct ttagccgcgc tgtcggatag accccgacgc tccgcgctcg
    19261 tcatgcccgc ctccgaaaca tcaaggtcga acgcctcgat gaaatccttg tcggcggtca
    19321 aattgcccgc ccgcaccacg cgttccgcaa gggattgcga cttcagaagc gaatattggg
    19381 tattgtagaa agcaagctcg ttgaccgtcg atcgttcccg gtcattttcg acggacgtat
    19441 ccaccggcgc atcgggaaga atttcaatcc gggcggtcga cgtgtattcc ggggtggcga
    19501 ggaaagtggc gagcaacccg aggagcagtg cgctcacaat cgcgccgacc acccagttcc
    19561 ggtgcgtcag aatcgaagcg aatatctttt cgatgtcggg aaacgcggac ggcgccgccg
    19621 cgccataggc ctcgccatat cgcggcgccg ctggatcaac cgacgtcatc attccctgac
    19681 cgtcggaaac ttggttcatc atgcgatcca tctctcttaa aactctctgg cgaggaggat
    19741 cagcggcgaa agaagcgccg gcgtcgccgc gagaatgtca cggaactgac ggcgctgcgg
    19801 cgaatccccg acaatgacga catcgttcgg aaaaatttcc ggatccgaat aattgccgcg
    19861 ccggatggct tccagattat agatgccgac atattgtttt tgaccgacat tccggaaaac
    19921 gaccacatcc tccagcttgg catattcggt gacgccgccc gccagcgcga cggcgcgcat
    19981 cagggtcatt cttcccgcca ccggatagga ccccgaattt ttcacctcgc catcgaccga
    20041 aacggccatc gattgaaaag ccagggcggt cgcgctggtc ggtgccctga aattcacggt
    20101 aacctgcggg tcccgaatat atttccctgc gagttggcct cgaagctcct cggcaagcgc
    20161 ggccggagtt ttgcctgccg cggcgacatt gccgaggaga ggaaaagaga aattcccctc
    20221 gccatcggtc atgatatcgc gctgcatttc gggaacaccg aaaacttcga tgcgaagttc
    20281 ggtaaatggg ccgataaacg ccagccgcgt ggaccgcacg acgtcggacg cctccggctc
    20341 gggcaaagcg gaaagaggga tgatcggcgt ccccggagcg ctcgccaccg ggggggcgct
    20401 tttggcacac cccgcgagaa gagccaaggt gacggaacac aggagaattt tattcatagg
    20461 agactgtcaa acccagtgct gcattggcgg tcaggctata gtggctcaat taaggcccgc
    20521 aaccctattc ccgttgcagg gcggccgtcc tcgggcacgc gaacaggacg ataaggatta
    20581 aggtcattgc ctgaagcgaa ggcgtgcgca gcggatagtc gaccacgctg gccgcggcga
    20641 acagccccac catcgaaagg atcggcaagc gatagtcgcc gcgataccct ttgatcaggt
    20701 tccggagccc cccgcgcacg gccgcgcgca ggaaccagac gccggctccg acaattatta
    20761 gcgcagacgg gaggccaccg gtgatcagga tttcgaccca gtcattatgc gcacggttga
    20821 agtaggcggg ctggagcagc gcgtccgatt cgaatatccg gtaaacggcg gggaaggatc
    20881 ccattccggc gcccgtcacc caataggttt cgatcatctg ctggatcgtc ggccaagcca
    20941 gaacgcgcaa atcctccgca acacggtttt cggctacacg gctcaatgac gtgctacgat
    21001 cggcaagcag caatatcgcc gccaaaagcc cggccaccaa caagggagga acgatcagca
    21061 tccaattggc catccacgat gcacgcgact tcgacgaccg gcgatgatcg tcccggccag
    21121 ggcggccgcg ccatgccggg agcagcatcg cgtagctcaa gacgaaggcc accgcccccg
    21181 cggccagccc cgcgcgcgag ccgatcagca tcgtcatgat cgtgcaaagc acggctgtcc
    21241 cggccaaacc cgcctggatc gcgggacgct gctgccggcg catcaattcg tcgcgcagca
    21301 acgtcgcggc gaacagaatc gcgcaagcct gaaaaacgcc atggtgattc cggtttgcga
    21361 agaggccgac catcgaaccc gtgttggtaa tacgatagag ataggcggga ctaccggcgc
    21421 cccaagcgat ctggagcaac ccgagcaccg cgttcacgat cgcgatggcg agcagcgcca
    21481 gcaggatacg gtgcatgtcc tcgggtccga gccgtgccgc gaccatcagc gcggccagcg
    21541 gcaccgtcat cgcaagcaag ctgttccagg tttcggacgg cgtcagcgtg atcggccgcc
    21601 aaagatcgga attacccagc aaccggtctg ccgcgacgat caattcgcgt cccggcagat
    21661 tgtgccagat cgacggcgac aacgggatca actggattgc cgtccatgtc gtaagagcca
    21721 gaagcagaat cagcggcacc cgcacgcgcc gccagtcggc gcgctgcaag ccgaccatcg
    21781 cccagcaggc aaacagaacc gccccgccgc ggagcaaagg cagcgaagcg atgtcgctcc
    21841 gcgagccgcc gccggtcaga aacacaaaga caaggaatgc gatcgctacc cagctctgga
    21901 gttcgacccc gtcgggcctc ttcgaacgcg cgcctcttcc tgttctctcg ttcatcgctt
    21961 cgctatctgg atcatttgct ggagatgcag ccgaggcaca tcgattgcgt gggggttaga
    22021 gggcggacag ccggatggca agcggtggtc cgcatcgccc cgcaggatga gttcagttcg
    22081 tcaagcggcg ttggcctgcg cctctgcttc gcgggtcagg ataaaccaac gccgatcttg
    22141 ctccaacccg atcgcggcca atgtaccgct gcgaaaggct ccgacgtcga ttccgatccg
    22201 gttggactgc tcgtcgggct cgctggttat gctatggccg tgcacgatca tcttaccatg
    22261 atcgcccagg tcatcgagga actcgtcgcg aatccaccgc aaatcggaaa gctgctgctt
    22321 ttcgaggggg atgcccggcc gcacgcccgc atggacaaag gcataatcgc cgatctcgat
    22381 cacatcctcg ccgcggccga ggaaatcgac atgctcggcc ggaaccagcg tcggcaggtg
    22441 cgccgccacc tcttcaaagc tggcttcggc aagactggca tcatcgcgcc aatagctgcg
    22501 gatcgtcgca tcgccgccga tccgcatgaa atagcgcaga cgctgcacgt cgccggtgag
    22561 cgccgcgaga aagcattcct cgtgattgcc gatcagcagc cgcgtgtcgg gaaattcgtc
    22621 tttgagccgt atcgcacgat cgacgactcc cgccgaatcg gggccgcgat cgaccaggtc
    22681 gccgagcagg atcagcgtga ccccggccgg atcgcgcttc gcattgtcgg cacggatcgt
    22741 gtcgatcatt tgcgcgaaca ggtcgtcacg accgtgaata tcgccaatgg cgtatacgcg
    22801 ctggccctcg ggtatcgcat gtcctccgcg cgccggtggc accgacttgg tcttacgctt
    22861 ccacaacatc actatcgaac cgatccaacg ccaatcatcc acgtcaacag gggaaaagcg
    22921 gccgtcaggc acgctcgcgt ccccctaata cccttgccgg gcaatgacaa catacgccgg
    22981 aaggggccga gagttgcgcc aacccgtgga aagcgcggca ggcggaggaa tttattgctt
    23041 aagggtgtcg aaagcattgc ctgttaaccc gcgacgcttc acgcctgtgc tcacaggaat
    23101 cgcgccgtgg cccggaggca cttcatgaag attatgacgg ttttcgggac ccgcccggaa
    23161 gcgatcaaga tgtttcccgt cgtccatgcc ctgcgcgaac aggccggaat ggacgttcgt
    23221 gtctgcgtga ccgcccagca ccgcgaaatg ctcgatcagg ttctcgaaat cgcacgcatc
    23281 acgcccgata tcgacctcga cgtcatgacc ccgaaccaat cgctcgacgc gctgctcgcc
    23341 cggctggtca ccggccttgg cgagacgttc gaccgcgaga agcccgaccg catcctcgtc
    23401 catggcgaca cactgaccac gatggcgggc acccttgccg cctatttccg caagattccc
    23461 gttggccatg tcgaggcggg gctgcgcagc ggcaacatct atcacccctg gcccgaggaa
    23521 gtgaaccgca aggtcgcggg cgcggtcgcc gaccttcatt tcgcgccgac cgaaaccgcg
    23581 gcggcggcgt tgcgcgccga aaatgtcgct gcggaccgca ttcacgtcac cggaaacacc
    23641 gtgatcgacg cgctgctcgc gacgaaagcg cggatcgatg aagatcccgc actcgccgcc
    23701 ggcctcgacg cgctggccgc ccgtttttcg ggcaagcgga tcatcgccgt cacctcgcat
    23761 cgccgcgaaa atttcggcga gggcatgaaa gccatcgccg atgcgatcgc ggggatcgcc
    23821 gcccgcgacg acgtcgcggt cgttttcccg gtgcatccca atccgcatgt gcgcagcgcg
    23881 atggaaccga tcctcggcga tctttcgaac gtcgcgctga tcgacccgct cgattatccg
    23941 catttcgtcc ggctgctcgg gatgagcacg ctcgtgctca ccgacagcgg cggggtgcag
    24001 gaggaggcgc cctcgctcgg caagccggtg ctcgtgatgc gcgagaccac cgagcgtccc
    24061 gagggcatcg cggcgggcac cgcgcgcctc gtcggcaccg ataaaaatcg catcgtttcg
    24121 gaaattttca gccttttgga cgatgaaggc gcctataatg ccatggcccg cgcccataat
    24181 cccttcggcg acggccgcgc ggcggaacga attgcggaga ttgttgcgcg tgcccattga
    24241 tagcgaacag acggtcaccg tcctcggcct cggctatatc ggcctgccca ccgccgcgct
    24301 gatcgcgcgc tcgggcaaca gggtcacggg cgtcgacgtc agccagcatg tcgtcgacac
    24361 ggtcaacaac ggcaaggtgc atatcgagga agtcgatctc gacgggctcg tccagggcgt
    24421 cgtgtcgcgc ggcgctctca tcgcctcgac cgaagtcgcg cccgccgacg tctttgttat
    24481 cgccgtcccg accccgcacg acgaggaaca tcgtcccgac atcggccacg tcctggcggc
    24541 cgcgcgggcg atcgcgccca agcttcaggc gggcaatctg gtcatcctcg agtcgacctc
    24601 gcccgtcggc accaccgaaa aggtcgccgc gctgcttgcc gagctccgcc ccgatctcaa
    24661 ggtccccggc gcatgcagcg gcgccgccga catcttcgtc gcctattgcc ccgagcgcgt
    24721 gcttcccggg cgtatcctcg tcgagctcgt cgacaacgac cgctgcatcg gcggcatcac
    24781 cccgcgctgc gcgcgccgcg cgatgacctt ctaccgccag ttcgtccgcg gcgcctgcgt
    24841 caccacctcg gcacgcgccg ccgaaatggt caagctggtc gaaaacagct accgcgacgt
    24901 caacatcgcc ttcgccaacg aactgtcgat gatggccgag catatgggcg tcgatgtatg
    24961 ggaggtgatc cgcctcgcca accgccaccc gcgcgtcaac atcctccagc ccggccccgg
    25021 tgtcggcggc cattgcatcg ctgtcgaccc ctggttcctc gtccatggag cccccgatca
    25081 cagccgcctg atccgcaccg cgcgcgaagt gaatctcgcc aagaccgcgc atgtcatcgg
    25141 cgcagcagaa atgctcgtgt cgcagcatcc gaaggcgcgc gtcgcgtgcc tcggcctcgc
    25201 cttcaagccc aatatcgacg atttccgcga aagccccgca gtcgaggtcg ccgccgcgct
    25261 cgcacgccgc ttcggcaagc agatcgagat cgtcgagccc tatgcgcgcg gcctgccgat
    25321 ggaattcgcg ggcaccggcg ccgaactgat cgacctcgac agcgcgctcg ccggctgcga
    25381 cctgctcatc gtcctcgtcg atcacgacct cttcaaatcg atcccgatcg aggaacgcgg
    25441 cgacaagatc gtctacgaca cgcgcgggct gtggctcgac cagccgaacg gcacggccgg
    25501 caatcggctg cacagggccg cctgacgctc tccgcttgac gccctcgcgg ggcgctcgca
    25561 tcctgacgcg atgctgaccc gcctcgaaac gatcgtcgtc gaaaagccgt ggggccgcac
    25621 cgacatcccc gccgatttcg gcgacttggg cggccgccgg attggcgaga tctggttcgc
    25681 caaccccgcc ggcgacgacg cgccgatcat ggtcaaattc ctcttcacct ctgaacggct
    25741 gtcgattcag gttcaccccg acgacaaagc cgcgcgcgac gccggattcc cgcgcggcaa
    25801 ggaggaatgc tggctgatcc tcaacgcgca gccgggcgcc gagctgggcg tcggtctcaa
    25861 ccgcgaaacc acgcgtgaga cgcttcacga cgccgcgctc gacggcagca tcgtcgatat
    25921 gatcgactgg cggccgtcga aggagaatga cttcgtgtac aaccgggcgg gcacgatcca
    25981 cgcgatcggc ggcggactca ccgtggtcga ggtccagcag aacgtcgatt gcacctaccg
    26041 cctctacgat tacggccggc cgcgcgagct gcatctcgat gaaggactag aggtctcgaa
    26101 ccccggtccg gtccacgacc cgcgcgatac ggtcgtcgat cccgcggcga accgcatgct
    26161 cgtgagcggt ccgcacttct atctcgccca gctcgccgcc ccgatcgatc cggccatcgt
    26221 cgaacaggcg acgggtgagc tgaccttcgt gccgctgtcg gccggctgcc gcgtcgcggg
    26281 cgaagctatc gcgctcggcg aagcggtgct gcttaccgat tcgaccggga tcgagatcga
    26341 tgagggcgcc cgcgcgttgc tgacctggcc tgcctgatcc tccctgtgcc gcgggcatgg
    26401 ggaggtggca gcgcgaagcg ctgacggagg ggctaatggc gcaacgttgt cgcccctcca
    26461 ccatcgcctg cggcgacggt ccccctcctc acggcttcgc cgcagggagg atcgagccgc
    26521 cctccccttg cggccccgcc gcccatcgcc catatcgcgc ggcatgacca atccccatgc
    26581 gcaaagtgcc gcgccctggc acggaaccac catcctttcc gcccgcagcg ccgacaaggt
    26641 cgtcatcatc ggcgacggcc aggtatcgat gggccagacg gtgatgaagc ccaacgccag
    26701 aaaggtccgc cgcctccacg acggcagcgt catcggcggc ttcgcgggcg cgaccgccga
    26761 tgccttcacg cttttcgagc ggctcgaggc caagctcgag cgccacaatg gccagttgct
    26821 gcgcgccgcg gtcgagcttg ccaaggactg gcgcaccgac aaatatctcc gcaacctcga
    26881 agcgatgatg atcgtcgccg acagggaggt cacgctcgtg attaccggca acggcgacgt
    26941 gctcgaaccc aagggcggga tcgccgcgat cggctcgggc ggcaattatg cgctttcggc
    27001 cgcgcgcgcg ctcgccgatt atgagaagga ccccgaggtg ctgacgagga aagcgatgga
    27061 agtcgccgcc gacatctgcg tctacaccaa cgaccagttc acgtccgaga ccatcgacat
    27121 caagggctaa gccactatgg tcgcgaggtg attcaagccc tccctcgccg ccatttcgtt
    27181 gccgcctccg ccgcgctcgc ccttccgggc tgcgcgacga ccacagcgcg cacgcctgca
    27241 tcgtgcctga tgcccgtcaa ggcgtcgccc gaccgcgtca tccgcaccct tgccggcctc
    27301 cgcccctatc gccccggcgg cttcgtcgtt cgcgccgata cgcttggcgc caaacggctc
    27361 gtccacaact acggccacgg cggctcgggg atcacaatga gctggggcac cgcgaagctc
    27421 gcggtcgaga tgggcgcccc cggccacaac ggccccgtcg cggtgatcgg tgccggcatc
    27481 gtcggcctgt cgaccgcgcg cctgctgcag gaggcgggct ttgccgtcac cgtctacgcc
    27541 aaggcgctgc cccccgacac gacctcgaac atcgccggcg ggcaattcca ccccttcggc
    27601 ctgttcaagt ccgatgccgt cacccccgaa tggcggcggc aattcaccgc ggcgctcgat
    27661 tatagctggc ggcgctttca gatcatggtc ggcgacgatt atggcgtgcg ctggctgacg
    27721 acctattccg aaggcacgcc cgaggagccc aagcttccga ccttcccgcc cgccgcgcgc
    27781 gcgcttcgcc ccggcggaca tcccttcccg gtcgaacggc tctggcaata tgacacgatg
    27841 tatgtcgaaa cgggccgcta cctccgccag atgatgcgcg atatcgaggg tgcgggcgga
    27901 cgcatcgcgg tgcgcggctt cgcctcgccc gccgagatcg cgtcgctccc cgaaacgctc
    27961 gtcttcaact gcaccgggct cggcgcgcgc gagctgttcg acgacatgga gttgatcccg
    28021 atccgcggcc agctcgccat cctcatcccc cagcccgaag tccgctatgc cttcaccggc
    28081 gaggcgggct atatgttccc gcgccccgac gggatcgtga tcggcggctc atacgaacgc
    28141 aatatatggg aagccgaacc gcagcccgag cgtatcgcgc gcatcgtcgc cgcacaccgg
    28201 cagctgttcg caggtttccg ctgcccggct tgacttggtc cgcccccttc ccatctagcc
    28261 gtcacatgaa attctttctg ctggacctct ccgacatatg aacaaagacc tgaccccgaa
    28321 ggcgatcgtc gccgcgctcg acacgcatat catcggccag accgccgcga agcgcgcggt
    28381 cgcggtggcg ctgcgcaacc gctggcgccg ccagcagctt tcgcctgatc tccgcgacga
    28441 ggtcagcccc aagaatatcc tgatgatcgg ccccaccggt tgcggcaaga ccgagatttc
    28501 gcgccgcctc gccaagctcg ccgacgcgcc gttcatcaag gtcgaggcga ccaaattcac
    28561 cgaggtcggt tatgtcggcc gcgacgtcga gcagatcgcg cgcgacctcg tcgaggaagc
    28621 cgtgcgcctc gaaaaggacc gccgccgcga agcggtacgc gccgccgccg aagaagcggc
    28681 gatggaacgg ctgctcgacg cgctcaccgg caagggcgcc agcgaggcga cgcgccagag
    28741 ctttcgccag cgcatccgcg aaggccatct cgacgatagc gaaatagaga tcgaggtcgc
    28801 cgacgccgcc atgtcgttcg aactgcccgg ccagccaggg cagatgagca tgatcaacct
    28861 ctccgacatg ctcggcaagg cgatgggcgg gccgcccaag aagcgccgca aattgaaggt
    28921 catcgacgcc gcgacgcggc tgattgagga ggaacaggac aagcgcctcg accaggacga
    28981 tgtcgcccgc gtcgcgctgg ccgatgccga agccaacggg atcgtctttc tcgacgaaat
    29041 cgacaagatc gccgtcagcg acgtgcgcgg cggctcggtg agccgcgagg gcgtccagcg
    29101 cgacctttta ccgttgatcg agggcacgac ggtcgcgacc aaatatggtc cgatgaagac
    29161 cgaccatatc ctctttatcg cgtcgggcgc ctttcacgtc gccaagccga gcgacctgct
    29221 ccccgaactc cagggccgcc tcccgatccg cgtcgaactc ggcgcgctct ccgaagagga
    29281 tttcgtccgc attctcagcg agacaaaggc cggtcttccc gaacaatatg cggcgcttct
    29341 cggtaccgag ggcgtgacgc tgaacttcac cgacgacgcc atcgcacgcg tcgcgaaact
    29401 cgcagccgag gtgaatgaaa aggtcgagaa tataggtgcg cgccgccttc agacgatcat
    29461 ggaacggctg gtcgaggaaa tcagcttcac cgccgaggac gcccccggca cgacgctcga
    29521 tatcgacgcc gcatatgtcg acagccaact tgccgatgtc gtcggcgaca ccgacctcag
    29581 caaatatgtc ctgtagagcg tatcggcagc actcggttcg agccgatcga attgcctatt
    29641 ttatccgggc agagagcgga gtaaaaagaa tgggaggtcc gcagacctcc catgccatag
    29701 tcttgactgt gtaaatgcag atatctcagc tggtttcgag ggcgctgatc acgccttcgt
    29761 acgtgacgac ctcaaccgat tgaagataga gcgacgccgc cagcgtgtca ttggcgtggc
    29821 cttggtcgcg tgtaacacgg atttccagat gaccgcccgg caagagattg tgcgacgaat
    29881 tagtattgat atagacgctt tcgacagtgc tgtcatagtt aacgtccaat cctggattgg
    29941 aaaatggagt accagctgga ccgcggcgta gatcgcggac aatacgtgca gtgcctgtcg
    30001 ccgcatctgg ccgcgtcata tagttgacgt ccactctacg cccacaatgt ttgggcatac
    30061 ggacaaaaat ggggccgaat gtcgtatcgc tcgacccttt ggtcatctcc aggtttgtag
    30121 aaaaaacttg cttgcgagga aatccaaagc gagcggtatc gttcgtcagc aaagttggat
    30181 aggcttcaat ggatccataa tagcgccctt cgatccaccc agtagcacgg cctactgtag
    30241 attcccggat acgaatcggc ggtgccgaat agccattacc gcctgacgtc aggaactgcg
    30301 gattgacgat ccgcaggtca tgcacgtcgt acagatcgat cgcccgttcg gcgatagtgc
    30361 cgttatattg ctgcggcgtg aaattactgt tgcgaatgat gatgctgttc gggccattcg
    30421 ggctggagcc gggttggtca tttcgaatat cgcgatcttc cggatgtcgt ccgttcttct
    30481 cgaaatacac gccgtcgacc agcaaaccgc gaacttcctc ggctaacacc aagccatctc
    30541 cgacgttgcc ctccaatgtc acaccgtaaa gattgacttg gtagccggag ccgacacgaa
    30601 tgccgcctcc cgaactcttg gcgctgcccc cgctaccgat cggcgagcca ccaatcgtgc
    30661 cgttgccctg catctggcct cctagaatgg tcagcgcgtt gcagccaaga tcgaactcaa
    30721 tcccttcgcg tccttcgata ccgaaattgg cgttgtagcg tgcgaacact ccaatcatca
    30781 gatattccca catatcggtc aggacaatgc cggaaccagc gcagttttcg acacgaacat
    30841 tcgacatcat cgcccgcacc ggatagcggt tcggagaccc gccggttggt tccaatacaa
    30901 tcccgtcagc gtccgccgag gagacacgca gatccctgat catatgcgcc ggcgaccgga
    30961 cccgaagcga accactagtg aactttaacc atgtgccatt gcgaccggcg ccgatgcccc
    31021 tgatggtaat ctggttccag tggttcgagc ccgatagatc ttcgatgaac ggattgacca
    31081 cgatgtcccc gtcaatttcg atcacgccgg gaggaagttg cagttcgagg ccgccattcg
    31141 atgtgacatg atctactgcc gcttgcaggg cggtcgcatt ggcagttgcg gatgcagacg
    31201 cggaaactcc ggcgatcgcg gcgttatatg caccttgatc gtcaatatat gattccagat
    31261 tccgcccatc gtctatcaag atttgtgcca tcttttcttt ctcctttgct ttatgagaac
    31321 aaaggttgaa caccgtatct cctacactgc atcctatttc gcgctgcttg tgtcctattt
    31381 cgtacaacgg cggctaccgc gcgccgccgc gcacccgatt caagcccgca caacaaaccc
    31441 aaacccctct cttttcagcc gtccatttac cgaacttcat ttgactttct cgcgccgtca
    31501 atgcgatgaa cgggtgacac ggtcgaacct gggtcaacgg ccggcacgga gacagcaatg
    31561 acggatgagg acgtcgcccc cgagcagggc gagttggcga tcacgcgcac cggcgatcgg
    31621 cttcggctgg cgcgggaggc ggcgggattg tcgctcgccg atgtggcgac gcggacgcgc
    31681 atcacccagc gccacctcgc cgcgatcgaa aagtcggatt tttcggaact tccgggccgg
    31741 acctatgtca ccggtttcgc gcgcgcctat gcccgcgccg tcgatctgcc cgaggccgag
    31801 atcggcgccg cggtccgcca ggaactcgaa gacgacgaat atggctcgcg gccgctttac
    31861 gaagcctatg agccgaccga tcccgcgcgt ctgccgacgg cgcggctgac ctggacgcta
    31921 gtcatcgtca cgctgctgct cgcgtcggcc tatggcgtct ggcgcttcct ttcggtggaa
    31981 cccgacgagg cgctgatcgc ggcgcagaac cgcgccgccg acgaatccga agcgccgcag
    32041 aacgtcgccg ccgcaacgcc ggcgaccaag gcgggcgctg ctgccattgc cgccaacgcg
    32101 ccggtcgtgc tgaccgtggt cggaccgacc gaagtctgga tcggtttcga cgacgccacg
    32161 ggcaagaccg agaattggcg cacgctcgac gcgggcgaga cctatcaggt tccgccggcc
    32221 tatgtcgaac aattcacgct gcgcacgagc atcccgcagg ccttgaaagt cacggtcggc
    32281 ggccgcgatg tcggcccgat cgggcccgcc gacacgctgg tcaagaatat ctcgctgaaa
    32341 cccgcggacc tcatcgcgcg cgccgaaggc ggcacgacga cgaccggcag gacagcaccc
    32401 ggaacggcgc cgcgccccaa aggctgaccg ggcgcatcga acggccggaa acgccggacg
    32461 ttcgacccgc atcgaaccgc gccgcgtcta aatgggtttg cgctggcgcc gattgccgcg
    32521 ttatggacgt ctcattggtt aatcagtggg aacccaacgg cagatgatga tgcgtcagat
    32581 cacctttctc ggaaccgcaa cgatcgcgct cgccgcggcc atgccagcgg cggcgcagga
    32641 caacaatatc ggcaagcgcg tcgagcggct cgaaaaggaa atgcgggcgg tccaacgctc
    32701 ggtctttccg ggcggtagcc cgaccttttt cgaaggcgag atcgcgcccg acaatacgcc
    32761 gggcgaacgc tcgcgcacca acgccccggt gatcgacctg accgcccgcg tcgacgcgct
    32821 cgaagcccaa ctacagacgc tgaccgggca gaccgagcag aatgcgttcc atctgcgcga
    32881 gctcgaaaag gcgtttaccg cctacaaggc cgaaatggac aaacgcttcg ccgatccggc
    32941 agcggtcgat ccggcgacag gcgatccgac cgcaaccccc gcgagcctcg cccccgtggt
    33001 aaaggcgccc gcgacggcca acgcgacgcc gacgaaaaag cccgccggca agcccgcagc
    33061 aaagcccggc gcccccgacg cggcacggct cgcgctcgtc aaaaaggtcg aagtgccgtc
    33121 gaccggcaac gagaccaagg acgcctatga ttatggctat cgcctgtggg acgcgaaact
    33181 ctatcccgaa gcgcaggcgc agctgaagac ggtcgccgaa aaatggccga agagcagcta
    33241 tgcgagcttt gcgcagaatc tgctcggccg cgcctatctc gacgaaggca agccgagcct
    33301 ggcggcggtc gccttctaca ataattacaa ggaccgcccg agcggcccgc gcgcgccgca
    33361 cagcctcgtt tacatgggca tcgcgctcga caagctcggg cgcaaggccg acgcctgcaa
    33421 ggcgttccgc gaactcgacg aagtctatgg cgacaaggcg ccgcaggatg tccgcaccga
    33481 tgctgccgcc gcaaaagcaa aagcaggctg ctgatcgcga cgtcgccggc cggctcgccg
    33541 gcggcatcgc ggcgctgcta ggccccgact ggcaccgact ccattacggc gtcgcggtgt
    33601 cgggcggccc cgacagcatg gcgctgctct ggctgatgac cagcctgctg cccggacagg
    33661 tctgggcggc gacggtcgat cacggcctgc gcaagggatc ggacgaagag gcgcggatgg
    33721 tcgcgggctt ttgcacgcgc gagcatattc cgcattcgac gctgcgcccg cctgcgccga
    33781 tcacgggatc gcagcaagcc gcggcgcgga ccgaacgtta tcgcctgctc gaacaatggc
    33841 gcgaagcgaa cgcgctcgac catatcgtca ccgcgcatca cgccgacgac cagctcgaaa
    33901 cgatcgcaat gcgcctcaac cggtcgagcg gcgtcggcgg gctcgctgcg attcgcgcga
    33961 aaaacggcct gattctgcgc ccgctgctcg attggcggcg cagcgagctg gtacaggtcg
    34021 cgctcgaaaa cgacctgccc ttcgtcgacg atccgtcgaa cagcgacgat cgtttcgacc
    34081 gcgcgcggct gcgccatgcg ctgcgatcgc agaccgcgct cgatcccgtc gcggcggcgc
    34141 ggtcggcggc gtggct
//
LOCUS       DATMSU010000036         2228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_57623, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000036 DATMSU010000000
VERSION     DATMSU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57623"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VN034_00960"
     CDS             <1..892
                     /locus_tag="VN034_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59195.1"
                     /translation="FDLPNLGSPAASAPRILIAGAGSNDGWRGADCWFVASPGSEPIP
                     VGSVRPAAALGLLAEPLTAGSDSLFDCSNTAVVELVNPSMMLESVDDAALLAGANRAM
                     VGGELLQFGEAEIVGPGIWRLSRLLRGRAGTAGAITHPAGEPFVLLDDPALLTLPDDL
                     AAMTVGGGAMLQWAPRNGTALAEIGVTAAGRALRPLSPVHAQIRPDGAGGVTIGWTRR
                     SRVDTGWRDHVDQPLGEAREMWRVSLVPPVPGVGPWERASPSLNISAGELAALAPGHA
                     IEICQAGDFSQSSPLFVALK"
     gene            902..1354
                     /locus_tag="VN034_00965"
     CDS             902..1354
                     /locus_tag="VN034_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HWV59196.1"
                     /translation="MTDMPITPRFALPLLAVAQAQKEVTHNEALTLLDALVHAAIEAG
                     PLATPPASADVGQCWIVGAAPTGAWAGQDHAIAIQTQGGWRFAAPREGMQVTRLDDRA
                     HLRFEGGVWVEPAMVAAPAGGSVIDSEARSAIATLITQLAARGLLISD"
     gene            1486..>2228
                     /locus_tag="VN034_00970"
     CDS             1486..>2228
                     /locus_tag="VN034_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWV59197.1"
                     /translation="MRKLAVAVALASTTLASPVLARDNSWYVGVGAGAMIVEDMDLDI
                     GTFNNAGTLDHRAGYDVEGTVGYDFGGFRAEVEVGFREADIKSGSFSAPGIPSNANGS
                     GTFTGSTDLNGDSNALSFMVNGMLDFGDDDGLQGFVGGGAGVARVSVEPVFAGPFLDD
                     SDTGFAWQAIAGIRAPLSDNIDVGLKYRFFNADNLDLVDQAGRDVSTRFRSHSILGTL
                     TFNFGGAPEPVAPPPPPPPPPPPPPPPPPP"
BASE COUNT          342 a          697 c          755 g          434 t
ORIGIN      
        1 gttcgacctg cccaatctgg gtagccctgc cgcgtccgcg ccccggatat tgattgcggg
       61 ggccggcagc aatgatggct ggcgcggtgc cgattgctgg tttgtcgcgt cgccgggaag
      121 cgagccgatt ccggtcggtt cggtgcgccc ggcggcggcg ctcggcctgc tcgccgagcc
      181 gctgacggcg gggagcgaca gcctgttcga ttgctccaac acggccgtgg tcgagctggt
      241 caatccgtcg atgatgcttg aatcggtcga cgatgccgcg ctgctcgccg gtgcgaaccg
      301 ggcgatggtg ggcggcgagc tgctacagtt cggcgaagcc gagatcgtgg ggccagggat
      361 atggcgcttg tcgcgtctgc ttcgcgggcg cgccgggacc gcgggcgcga taacccatcc
      421 ggcgggcgag ccgttcgtcc tgctcgacga tccggcgttg ctgacgctgc ccgacgatct
      481 cgctgcgatg accgtgggcg gcggtgcgat gcttcaatgg gcgccgcgga acggcacggc
      541 gctggccgag ataggtgtca cggcggctgg acgggcgttg cgaccccttt cgccggtgca
      601 cgcgcagatc cgccccgacg gggctggcgg cgtgacgatc ggatggacga ggcgcagccg
      661 cgtcgacacc ggctggcgcg accatgtcga ccagccgctg ggcgaagccc gcgaaatgtg
      721 gcgtgtttct ctggtgccgc cggttccggg cgtgggacca tgggaacgag catcgccctc
      781 tctcaacatc agcgccggcg aactggccgc gctggcgccg ggccatgcga tagagatttg
      841 ccaggcaggt gatttttcgc agtcgtcacc gcttttcgtg gctttgaaat aaggacagat
      901 catgaccgac atgccgatca cgccgcgctt tgcgttaccg ttacttgcgg ttgcgcaggc
      961 ccaaaaggag gtgacgcaca atgaagcgct taccttgctc gatgcgcttg tccatgcggc
     1021 gatcgaagcg gggccgcttg cgacgccgcc ggcgagcgcc gatgtgggcc aatgctggat
     1081 cgtcggcgct gcgccgaccg gggcttgggc cggacaggac catgcgatcg ccattcagac
     1141 gcaggggggc tggcgatttg cggcgccgcg agaggggatg caggttaccc gtctcgacga
     1201 tagagcgcac cttcgattcg agggtggcgt ttgggtcgaa ccggcaatgg tcgcagcgcc
     1261 cgcgggaggc tccgtgatcg attccgaggc gcggagcgcg atcgcgacgc tgatcacaca
     1321 actcgccgct cgcggtcttc tgatttcgga ctgaatttcc gttcgttggc gaacaaagtg
     1381 cgactttttg gcaacagatt gacgatttgt ttcacttgca cggaaccaaa gcggcgagta
     1441 ggacgtctgc cgagacgtat atctcaaatt gaaaggggaa ttactatgag gaagcttgcc
     1501 gtcgctgtgg cgttggcctc caccaccctg gcgtcgccgg ttcttgcgcg cgacaactcc
     1561 tggtatgtcg gtgttggtgc cggtgcaatg atcgtcgagg acatggacct cgatatcggc
     1621 accttcaaca atgcgggtac gctcgatcat cgtgctggct atgatgtcga aggcaccgtc
     1681 ggttacgatt tcggcggttt ccgcgcggaa gtcgaggtcg gtttccgtga agccgacatc
     1741 aagtcgggca gcttttcggc ccccggcatt ccgagcaacg ccaatggctc gggcaccttc
     1801 accggtagca ccgacctgaa cggcgattcg aacgccctca gcttcatggt caacggcatg
     1861 ctcgacttcg gtgacgacga cgggcttcag ggctttgtcg gcggtggtgc cggtgttgct
     1921 cgcgtttcgg tcgaacccgt tttcgcaggc ccgttcctcg acgattcgga cactggcttt
     1981 gcctggcagg cgatcgcggg catccgcgct ccgctcagcg acaacatcga cgttggcctg
     2041 aagtatcgct tcttcaacgc cgacaatctc gatctggtcg atcaggccgg tcgcgacgtt
     2101 tcgacgcgct tccgttcgca ctcgatcctc ggcacgctga cgttcaactt cggtggcgct
     2161 ccggaaccgg tggcaccccc gccgcccccg ccgcctccgc cgcctccgcc gcccccgccg
     2221 cctccgcc
//
LOCUS       DATMSU010000037         8986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_86171, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000037 DATMSU010000000
VERSION     DATMSU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86171"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1596)
                     /locus_tag="VN034_00975"
     CDS             complement(259..1596)
                     /locus_tag="VN034_00975"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWV59198.1"
                     /translation="MYRFILAANIAAIALVPSVAGARTQSQATCEEQRSTRVVATIGG
                     AAAGGVLGNVVAGRGDKTIGTVVGAAAGAVIANQIAKPTRDCRDAFGYYDDQNRWHAT
                     GVNSSDARGYYNREGIWVDGPPNGRYGSDGRWVTYAASSRGEGDYRSGGEWVPASANG
                     YYDRNDTWVPGSAGGRYDDRGRWIGTAASAPQNRRDDAYGYYDTTGAWHATSTARGRA
                     TGYYDRDNNWVTGTPNGYYDERRNWIPQREDGTASGSYDGQNRWIPASSAGYYDDNDR
                     WISGAASGHYDPRGRWVAGVTVGHYDMNGRWIAGAANGRRNANGNWVADPHPGYYDRN
                     GRWNAGTTTGYYDARGRWVPTGREGMGSDYGTNRPPILSQLANLDQYVRTARSERTIN
                     NRTATSATRELRSIRASERAMRHDRMDRLSARNEAALQSRIDRLNLRLRLSPQ"
     gene            3054..3206
                     /locus_tag="VN034_00980"
     CDS             3054..3206
                     /locus_tag="VN034_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59199.1"
                     /translation="MILHTLFGRHLVDFKASHWDRGRFVSHCTVCGCEMVKLPGLIWQ
                     ASGRRV"
     gene            3859..4065
                     /locus_tag="VN034_00985"
     CDS             3859..4065
                     /locus_tag="VN034_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWV59200.1"
                     /translation="MTTGTVKFFNADKGYGFIAPNDGGQDAFVHISAVERAGMHTLQK
                     DQSVSYELETDQRGKTSAVNLQAA"
     gene            4228..4656
                     /locus_tag="VN034_00990"
     CDS             4228..4656
                     /locus_tag="VN034_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59201.1"
                     /translation="MSLSIMPMTPGAGASRYNVGKGLRSSLDFIKGAPSLPTLPGKST
                     ALSCREPGTEGFEMNDVPGLGVMNLIEAQEASDVSAINGILSLANILRKRGLLSDAEA
                     SAMYESMSLPLGLPKYAENPDVQDIQSNLDRLFAVVMQPK"
     gene            complement(4874..5245)
                     /locus_tag="VN034_00995"
     CDS             complement(4874..5245)
                     /locus_tag="VN034_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWV59202.1"
                     /translation="MAEVDESTQLLCQSNKADAMTVKTRDGDKLGHIHAFMINKRSGH
                     AVYAIVTIGGFLGMGKAYYPLPFRLLAFEATSDSYVVTIDRRVLEGGPSWAANAPLFD
                     QAYADRVASYYEVAKEDLTLG"
     gene            complement(5696..5914)
                     /locus_tag="VN034_01000"
     CDS             complement(5696..5914)
                     /locus_tag="VN034_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59203.1"
                     /translation="MWESAVNDVSARTVLVVEDDPLVLLSAVAMIEDAGYDVLEAANA
                     DEAIALLEAIRRSASCLPISKCPDPWMD"
     gene            complement(6139..6582)
                     /locus_tag="VN034_01005"
     CDS             complement(6139..6582)
                     /locus_tag="VN034_01005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWV59204.1"
                     /translation="MRDYFDQLCKSLGALMINDPAELSLTVDVDESRVNADISVSLGL
                     IVTELVINALKHAFPGERGGAIIVDYHAMGKSWKLSVGDDGVGTMAKLADAKPGLGTS
                     IVAALANQLDVTVTTENGEPGTKVSIVHDEPTGRCHNDKGFGRVS"
     gene            complement(6663..7592)
                     /locus_tag="VN034_01010"
     CDS             complement(6663..7592)
                     /locus_tag="VN034_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWV59205.1"
                     /translation="MSRLALRDFDAVLAVARRGSFRQAAIDLGISTTALSHMIARIEA
                     ELGVRLFNRTTRSVALTDAGRIFIDRLGPSLQDVRETIEIVRERRATPTGTLRINAPP
                     LAARSPALTNLLVEYSRRYPDMHVDVVTEGQLRDIVAEGFDLGIRVASLVPTDMITLS
                     LGEAQRFAVVASPAYLATRSRPLVPTDLPSHDCVRVRLPDGSLYRWHFEKGGDAAQVE
                     VSGPMTFDDLTLSRAAVLAGFGIGFFFEQDVIADIEDGKLVRLLEDWTLPFPGLCLYY
                     PGRRHPSAGLAAFLTLARELASNARGPGRAASS"
     gene            7691..8068
                     /locus_tag="VN034_01015"
     CDS             7691..8068
                     /locus_tag="VN034_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59206.1"
                     /translation="MHTHNASGSSQRVAKALVATLPKPIADYIEANSRLDVVGMLTPF
                     AADAVIVDNGHRHEGYAEIRNLFEEEVIAVKAIFVPDFVRHEKSKIVVEGSAHGDFKG
                     SPIRFTYDFTLDNDSIKTLEITL"
     gene            8268..8852
                     /locus_tag="VN034_01020"
     CDS             8268..8852
                     /locus_tag="VN034_01020"
                     /inference="COORDINATES: protein motif:HMM:NF005095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59207.1"
                     /translation="MSAAALAHMGGVDILVHVIGGSSSPGGGFVALTDDHWLTELNLN
                     LLATVRLDRLLVPQMIERGSGAVVHVSSIQSVLPLPESTTAYAAAKAALRTYSKSVSK
                     ELGPKGVRVNIVSPGWIMTEASEELLKRLQAANGGTIEEARQLVLDGLGGIPIGRPAD
                     PHEVADLIAYLASDRAAAIHGAEFVIDGGTVPTV"
BASE COUNT         1745 a         2717 c         2737 g         1787 t
ORIGIN      
        1 gtcgcgagct gtcatcgtcg ccccatcggt ttcgaacgtc cgcttcttca ggctcgccgg
       61 ctgaaacccg ccaggctcca accggccagc cgctgctttg ttgagcttta cctaatctgc
      121 atgaaatagt tcgagcggag cggggctccc ggctcgagac ccccgaatgc cgcctcgaac
      181 atttcgtcgc cgagggacga taggctgccg cccccgcacc gagcggaagc ggcagatccc
      241 ctttaatggg cgttcgtttt actgcgggct gagccgcagg cgaagattca ggcgatcaat
      301 cctcgattgc agcgcggctt cgttgcgtgc tgacagccga tccattcggt catgccgcat
      361 ggcccgctcg ctggcgcgaa tggatcgcag ctcgcgggtg gcactcgtgg ccgtccggtt
      421 gttgatcgtg cgctccgatc gggcggtgcg gacatattgg tcgaggttgg cgagctgcga
      481 gagaataggg gggcggttcg tcccataatc gctgcccatg ccctcgcgcc cggtagggac
      541 ccagcgccca cgggcgtcgt aatagccggt cgtcgtgccg gcgttccatc ggccgttgcg
      601 atcataatag cccggatgcg gatcggcgac ccaattgccg tttgcattgc ggcgtccgtt
      661 cgcagcaccg gcaatccagc gaccgttcat atcataatgg ccgacggtca cgcccgctac
      721 ccagcgaccg cgcggatcgt aatgcccgct cgccgcaccg gatatccagc gatcattgtc
      781 atcataatag cccgccgagg aggccggtat ccagcggttc tggccgtcat agctgccgct
      841 cgcggtgccg tcctcgcgct gggggatcca gttgcgccgc tcgtcataat agccgttcgg
      901 agttccggtg acccagttgt tgtcgcggtc ataatagccg gtggcccgac cgcgcgcggt
      961 tgaggtcgca tgccaagcgc cggtggtgtc gtaatagcca taggcatcgt cgcggcgatt
     1021 ttgcggtgcg gaggcagccg ttccgatcca gcgtccgcga tcgtcatacc ggccaccggc
     1081 cgaacccggc acccaggtgt cgttacggtc gtagtagcca ttcgccgatg ccggcaccca
     1141 ttcgccgccc gatcgatagt cgccttcgcc gcgactggac gccgcataag tgacccagcg
     1201 accatcgctg ccatatcggc cgttgggcgg gccatcgacc catatgccct cgcgattgta
     1261 atagccgcgg gcatctgagg agttgacgcc ggtggcgtgc cagcgattct gatcgtcgta
     1321 atagccgaag gcgtcgcggc aatcgcgcgt cggtttggcg atctggttgg cgataacagc
     1381 tccggcggca gcgccgacca cggtgccgat cgtcttgtct ccgcgtcccg cgacgacgtt
     1441 accgagcacg ccgccggccg ccgcgccacc gatggtggcg acgacgcgcg tcgagcgctg
     1501 ttcttcgcag gtcgcctgcg actgcgttcg tgcgccggcg acggacggaa caagggcgat
     1561 ggcggcgata ttcgccgcga gaataaacct gtacatttgg gggctcctgg aactggcaaa
     1621 atgccgttcg ctttgaatga acgcccggcg cctgattttg ttccttaagt ttcagtttta
     1681 tggaataaca ttcccaaata ttgaaatatg tgatcctatc attataaatt atatggataa
     1741 tcttgattga attttattta tcttttttaa cgtaaaggag aaggcggtgg ggagatttga
     1801 taaagcgttc gcgccagaaa ctcgcatctc tctttatgcc ggacatgaat taggaaaaga
     1861 atgacgcgca atagggagcg gagcgcgcct ggggcgcact ttcgacgcaa gtcgccggtc
     1921 gtcggcgatc agtggcgccc tgcgttccat atcgccgtca attggcgaat gacccgcatg
     1981 cgatgttgat aggtgctata tcgttttccc acacgcccat tggcgaagtc gtcggcgagt
     2041 ttacgggtca accagtctat ttccgctctc tgcgtgtcgg agtcggtcac cgccgtttgg
     2101 ggacccgagg cgctggggtt tcggcagggc gtgaacgcgg ggaagtgacc gccttcattt
     2161 tcccaactct ccacggcgga tcgccggtca gcctctggta gcaatggaaa cgccatggca
     2221 tgcggtgcgg ttacggccag ctcaggcttc aaaacgtctg atatgttcgg catgacccga
     2281 ctccataggc gagcgggagt atgggatcat ctctctgtcg caccggcgcc cggggcatgg
     2341 gatgcgatgt ttcccatgtg gggatgatgc aggtcaaatg ctagggcgga gccatcctat
     2401 tcggcgacgg aacgaaagag tatcggggga agctcgtctc ctggccggct atgcgctgtg
     2461 cggcttcggc acttcgaaaa cgcgcgcgac ggccgtcgtt cagaacgggc gcatgtccgg
     2521 ggaaggcggc gccttcgagt gccatcccga tataccgtcc cgcaaagcgc cgggactgcg
     2581 ccggcgccgg aggaatgatg acgatatggg ctgataccga tgagcggacc gatgctccgg
     2641 tgcgaattga tgcctgtaat gcgtccaccc tgtgataaga tgcgctatca cccgatttcg
     2701 atcgcaagct tcccgtgact gagagacatc gagctcccgc ttgcccgccg ggagttttcc
     2761 gatgatcgct attcctgaac cctcgcgacc tttcagccgg gtcgttcggc tgtggagccg
     2821 cctggttcgc catgtactgg aacatgtccc gcaaacgacg gaaaatgcgt tccgccagtc
     2881 tccgcggcgg caggccgatc tggaacgcgc gctcgatagc tgggcgagcg ataacggcac
     2941 ccggccagga tcgccgcaac ccgcctgtcc gcaagatggt ctcccggaac cctttcgtca
     3001 tgcgctcgat cgctgggaca atgaggcgca caacctgagc gaggagcgcg atcatgattt
     3061 tgcacactct tttcggccgc catcttgtcg acttcaaggc cagtcactgg gatcgggggc
     3121 gcttcgtcag ccattgcacg gtgtgcgggt gcgagatggt caaactgccc ggcctgatct
     3181 ggcaggcgag cgggcggcgg gtctgaggcg attcgcttct gccgcccgac cggacggaag
     3241 cgctctgttc gtcgtccgac atgatcgtca ggaacaatgc cctatcccgc ccattagcga
     3301 gcgtttgaag aggggagaag ggtgatagtc cagtgtcagg aatttcaagt ccggtctcgc
     3361 gttgcttctg catcgcacag gagcctcccg gcgttttcgc agaccgtcac attgttgccg
     3421 cggcaccggc cggaatccgc atgtggcaag gagtgggaca atgttcagag gggccattac
     3481 agggcggcgt tgcctcactg gaaagcgctg tagcgcttcg tacccctttg gctcgacaat
     3541 tccttgcccg cttccagccg gaaaataatg acgtgctaag cgcaatggga tgaaatattc
     3601 tgccatcgca gctaaaatta tgtcgctgat cgctcgatac ggatcgtcct tgcgtgaaaa
     3661 tagccatgcc ggcttttcgt aaaaaagcag aattatcttt caattttcga tttaactgat
     3721 attggggtga tgcttacgtc gcatgcgacg gataatcggc agcttcatgc catcccttta
     3781 tccctttcta gcactattga agccgggacg cgtcgctgtt ggcgcatgcc cgtcgtattt
     3841 gggataaagg atacccatat gactaccgga accgtgaaat tttttaacgc cgacaagggc
     3901 tatggcttta tcgcgccgaa cgatggcggc caggatgcct tcgtgcatat ctcggccgtc
     3961 gaacgcgccg gcatgcacac ccttcaaaaa gatcagagcg tatcatacga actggagacc
     4021 gatcagcgag gcaagacctc tgccgtgaat ctccaggctg catgatcctc tggcggcggg
     4081 ctgcatcagc gcccgccgct atccatatgg gccctggaaa tgccgcccgc gtgtgggctg
     4141 cgatcgccga aaatccgcca tgcgccagct tgctctcatt tcgttcaggc gaacggccct
     4201 caaatggcgc ccgcaacgat ccatgagatg tctctatcca tcatgccgat gacgccgggc
     4261 gcgggggcaa gccgatacaa tgtcggtaaa ggtcttcgat cgtcgctgga ttttataaag
     4321 ggggcgccat cgcttcctac cttgccaggc aagtcaaccg cgctttcatg tcgagagccg
     4381 ggaacagaag gattcgagat gaatgatgtc cctggccttg gcgtaatgaa ccttattgag
     4441 gcccaagagg catccgacgt ctcggcgatc aatgggattc ttagccttgc caacattctg
     4501 cgtaagcgcg gcctgctaag cgatgccgaa gcttcggcca tgtacgagag catgagcttg
     4561 ccgctcgggc tgcctaaata tgccgagaac ccggatgtac aggatatcca gtcgaacctt
     4621 gaccggttgt tcgcggtggt catgcaaccc aaatagtcgg gccatgcaaa cttccacctg
     4681 ggcggacgtc cgactaagga aggccgcttt cggcaattcc gtgtggccgg ggagccccca
     4741 gcgcgaagcc ggctaagatg cgccgcgacg ccatccaagc atcactcccg ccgcgcctga
     4801 atctttctga caggcctgtc cggctgctct caaggatcga attcttgacg tcgctcggtg
     4861 acaaaaagca ccattacccg agcgtcagat cttccttcgc cacctcataa tagctggcaa
     4921 cacggtcggc ataggcctgg tcgaacagcg gcgcattggc ggcccagctt ggcccccctt
     4981 cgagcacgcg ccggtcgatc gtgacgacat aggaatcgct tgtcgcctcg aatgccagca
     5041 agcgaaaggg caacgggtag taagctttgc ccatgccgag gaaaccgccg atggtgacga
     5101 tcgcatagac cgcatgcccg ctccgcttgt tgatcatgaa ggcatggata tgccccagct
     5161 tgtcaccgtc gcgtgtcttg acggtcatcg cgtcggcctt gttcgactgg catagcaact
     5221 gggtcgattc gtcaacttcg gccatgggtt atcctttgat cgatttcgca gcaggccagc
     5281 gaaacagggt gagaggtcaa gcgcgccgcc attgcttcca gatggcccgg tttgacgcgc
     5341 gcctgcttga gcgcagtttt gcgattaccg ctatcgtggc gacgagaagg gcggcaaggc
     5401 ctgatgctat gagccagggc gtttcgatga cgatgtctcc ggcacaaaag gcccctctgc
     5461 tgttctacgc ttggcgcgga caaaggctgc ctcgctcccg tcgatcccgg agcggactgg
     5521 cgcgcttacg gtaccggcag gaagacactt aggtgcgccg caaggtctcc cgaccgatag
     5581 ggtttggcga gaaaaacgcc gtccgcgggt aaaagaccct cgcctggaag gatacgcccc
     5641 gaggtcacga taatcgccac gggcggccag ccatcgcgga cataggccgc cagtttcaat
     5701 ccatccatgg atccgggcat ttcgatatcg gtaaacacga cgcggatctg cggatggcct
     5761 cgagcagcgc gatcgcttcg tcggcattcg cggcttcgag aacatcgtag cccgcatcct
     5821 cgatcatggc gacggcggag agaaggacaa gcggatcgtc ctcgacgacc agcaccgtcc
     5881 tggcagatac atcattcact gcactctccc acatccaaac tcacatcgtc gacccgagcg
     5941 acgctccgtc ttcacgtttt tggacgatcg aaggcgcaac gtcctgctgc ccaataggtc
     6001 cccgtgtcac gaaaactaat gtttccaagg gtggaagggc aggagcgatc acgtaggtct
     6061 gcggctgacg ggaacttgcg gtccgctcca gcgtaatgaa ggcaaccccg tctcctgacg
     6121 cctcttgtct cacggttttc aggagacgcg gccaaagccc ttgtcattat gacatcggcc
     6181 cgttggctca tcatgaacga tcgacacttt ggtgcccggt tcgccatttt cggtcgtgac
     6241 cgtcacgtca agctggttcg ccaacgcagc gacgatgctg gtgccgagac cgggcttcgc
     6301 gtcggccagc ttcgccatcg tcccgacgcc atcgtcaccg accgacagct tccagctttt
     6361 gcccatcgcg tgataatcga cgatgatcgc gccgccgcgc tcgcccggga aggcgtgctt
     6421 aagcgcattg atcaccagtt cggtgacgat gagcccgagg ctcaccgaga tatcggcatt
     6481 cacacggctc tcgtcgacat cgaccgtcag tgaaagctcc gccgggtcgt tgatcatcaa
     6541 tgcaccgagg ctcttgcaga gctggtcgaa gtaatcgcgc aactccactt cgccgagctg
     6601 cccatgtagg cggctgggac ctcacggccg aaccgaacgg ctgaacagtc gcgccgctgc
     6661 aatcatgatg acgcggcacg gccgggaccg cgcgcgttgg aagcgagctc gcgcgccaaa
     6721 gtgagaaagg ctgcgagacc cgcagaaggg tgtcttcgac ctggataata gaggcagagg
     6781 cccgggaagg gcagtgtcca atcttcgagc aagcggacaa gctttccgtc ttcgatgtcg
     6841 gcaatgacgt cctgctcgaa aaagaaaccg atcccgaagc ccgccagcac cgcggcacgc
     6901 gatagcgtaa gatcgtcgaa tgtcatggga ccgctcacct cgacctgcgc tgcgtcgcct
     6961 cctttctcga aatgccagcg gtagagcgag ccatcgggca accggacgcg gacacagtcg
     7021 tgggaaggaa gatcggtcgg caccagcggc cgtgatcgcg tggccagata ggcgggcgag
     7081 gcgaccacgg cgaagcgctg cgcctcgccg agcgacagcg tgatcatgtc ggtcgggacg
     7141 agcgaggcga cgcgaatgcc gaggtcgaag ccttcggcca cgatgtcgcg tagctgcccc
     7201 tcggtcacca cgtcgacgtg catgtcggga tagcggcggg aatattccac gagaagattg
     7261 gtgagggcgg gcgagcgcgc ggcaagtggc ggcgcattta tccgcagcgt tcccgtcggc
     7321 gttgcacggc gttcacggac gatctcgatg gtttcgcgaa cgtcttgaag cgagggacca
     7381 aggcggtcga tgaagatccg ccccgcgtcg gtcaatgcga cgctccgcgt cgtccggttg
     7441 aacaggcgca cgccaagctc ggcttcgatc cgcgcaatca tgtgactgag cgcggttgtc
     7501 gatatgccca gatcgatggc ggcctgccgg aaggatccgc gccgcgcaac ggccaacacg
     7561 gcatcgaagt cccgaagagc aagtcgcgac attatcccga atccttcact agctcatgcc
     7621 atattgacct acttatccgg atagtgtgct gatcttacaa cggaggcaag tcaaacgaag
     7681 gaggcatgcc atgcacaccc acaacgcatc cggttcgagt cagcgcgtcg cgaaagcgct
     7741 tgtcgcaacc ttacccaagc ccattgcaga ctatatcgaa gccaactctc ggctcgacgt
     7801 cgtcggcatg ctgacgcctt tcgccgccga cgccgtcata gtggacaacg ggcaccgtca
     7861 cgaaggctat gccgaaatca ggaacttgtt cgaagaggag gtgatagccg taaaggcgat
     7921 cttcgtgccg gatttcgtgc gccacgagaa aagtaagatc gtggtggaag ggtcagccca
     7981 tggtgacttc aagggcagtc caatccgttt cacctatgac ttcacgctcg acaacgattc
     8041 catcaagacg ctggagatca cgctatgagc atcgcggctg atccagccgg gttcgctgac
     8101 aagcgcgtag ttgtcagcgg cggaaccaag ggtttgggcc gcgccaccgt cgaacgcttc
     8161 ctggcgggcg gcgcgagggt gatgactgca gcccgcggca accctgcccc cctagcggtg
     8221 cggagttcgt ccgggcagac ctgacgacgg ccgcgggagg cgaaagcctg tctgcagcgg
     8281 ctctcgcgca catgggcggc gtcgacatcc ttgtccatgt gatcggcggt tcatcctcac
     8341 ccggcggcgg ctttgtcgcg ctgaccgacg atcactggct taccgaactc aacctgaacc
     8401 ttctggcaac cgttcgcctt gatcgcctct tggtgccgca gatgatagag cgggggagcg
     8461 gggccgtggt gcacgtctcc tccatccagt cagtcctgcc ccttcccgag tcgacgaccg
     8521 cctatgccgc cgccaaggcc gcgctcagga catacagcaa gtcggtgtcc aaggaactcg
     8581 ggccgaaggg cgtgcgggtc aatatcgtct cccccggctg gatcatgact gaagcgtccg
     8641 aggagctgct gaagcggctg caggccgcca atggcggcac gatcgaggag gcgcggcaac
     8701 tcgtcctgga tggcttgggc gggatcccga tcggtcgtcc ggccgatccc catgaagtcg
     8761 ccgatcttat cgcctatctg gcttcggatc gcgccgccgc tatccacggc gccgagttcg
     8821 tcattgacgg cggcaccgtt ccgaccgtct gagtttatcg ccccgacgtt cgcacctcgt
     8881 gcggacgtcg ggaaagcgcg ggccggcggt gtcggtctcc gcgcttggag aacaggcaga
     8941 agatttttgg cgctctgagc tgatccggta cggcggagac gtaatc
//
LOCUS       DATMSU010000038         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_180945,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000038 DATMSU010000000
VERSION     DATMSU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180945"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VN034_01025"
     CDS             <1..529
                     /locus_tag="VN034_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59208.1"
                     /translation="ITGMPSPDGTLPMVLVLIAGLGVFALAMWWDRSDRVRQTRRSDV
                     AFWLHLLAAPMIAHPIFHLLGVTDGGNIGSGAAVLVIGVYILFGLIALAIDRRALLVS
                     ALAYVLFAMTQLFRTFGAVELNVALTAFVIGSALLLLSAFWQNARAVVVGLLPDDLAN
                     QLPATIRTASVPAHG"
     gene            822..1532
                     /locus_tag="VN034_01030"
     CDS             822..1532
                     /locus_tag="VN034_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV59209.1"
                     /translation="MTPPNILMVEDDPPIRTLTARALQEHGYIVRTAGTAPQMWDALR
                     AAPADLLLLDIMLPGTSGIDICREVRRTSDVPIIFLSAKGTETDRIVGLELGADDYLA
                     KPFGVRELVARVRAVLRRHAIERPVTDRERGLIRFDNWTADLPRRQLTSPDGAAVELT
                     GAEFDLLVSLCDNAQRVIARERLIELSRTRLGDSSDRSIDVLISRLRRKLSNPGKPAP
                     IVTVRGIGYMLNVPVERL"
     gene            1529..2848
                     /locus_tag="VN034_01035"
     CDS             1529..2848
                     /locus_tag="VN034_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV59210.1"
                     /translation="MSLFEKRGFGLFGQIVAILFAAVLLESAASIFLYERASQFSING
                     DKARRLAEHLVLSGKLIEEAPRSQRDTVASELTTTRYHLVWQERLVNPPPVSPKLNQI
                     TRQILDWEPEMQDRDLRLYLQSPGLHSTISGAIRLNDGSWVSFSARDHVRKLDLTLFR
                     TLQTLVPAIALVLLASLLIRHTLRPMRDLTRAAETLGKRDFVDVPLRGPAEVRKVIDA
                     FNDMQHRITAMIADRTQALAAVGHDFRTPLARLRLRADSVEDPSVGIAMEQDIGEMER
                     MIDSLLAYLSGDIDDPTEPVAPTDIAVMCATAADEASDRGHRVRYAGPDHLVLRVHGI
                     AIKRALLNLVGNALRFGNKIEVRLRSGERNIEIEVADDGPGIPEHLREEAVQPFARLD
                     SARARDTGGFGLGLSIVERVARMHGGSLELGQSHLGGLAARLRLPAA"
     gene            2931..>3584
                     /locus_tag="VN034_01040"
     CDS             2931..>3584
                     /locus_tag="VN034_01040"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWV59211.1"
                     /translation="MNDLIGRVFDFEKRVFPSRNDLFAKLVADGQSPKALMISCADSR
                     VAPEHVMQAEPGDMFVCRNAGNIVPPFATQNGGVSSTVEYAVAALGVRDIIVCGHSDC
                     GAMKALMNPHMMEGMPNVAAWLRHSHAAEHVVRSGYTDLDDAGLVRAASLENIVVQLA
                     HLRTHPSVAAGIARGEISLHGWFVDIHLGEVMGLDGASGQFVPIRANEPLPVALAAAQ
                     "
BASE COUNT          573 a         1244 c         1155 g          612 t
ORIGIN      
        1 gatcaccgga atgcccagcc ccgacggcac gctgccgatg gtcctcgtgc tcatcgccgg
       61 gctcggcgtc ttcgcgctcg ccatgtggtg ggaccgcagc gaccgagtgc gccagacgcg
      121 ccgcagcgac gtcgccttct ggctccacct gctcgccgcg ccgatgatcg cgcatccgat
      181 cttccacctg ctcggcgtca ccgacggcgg caatatcggc agcggcgccg cggtgctggt
      241 gatcggcgtc tatatcctct tcgggctgat cgcgctcgcg atcgatcgcc gcgcgctgct
      301 cgtctcggcg ctcgcctatg tgctcttcgc gatgacgcag ttgttccgca cctttggcgc
      361 ggtcgagctc aatgtcgccc tgaccgcctt cgtcatcgga tcggcgctgc tgctgctctc
      421 ggccttctgg cagaatgcgc gcgcggtcgt cgtcggcctc ctgcccgacg atctggcgaa
      481 ccagctgccc gcgaccatcc gcaccgccag cgtcccggcg cacggataga agcgaccaaa
      541 cgcgccggct tccgcccctg gaagccggtg ttcggggctc cggccggatg gccggggccc
      601 cttttcgtcg ccgtcatgtc ggccaccgca cacccggaaa tgattcgccc ccttgcctga
      661 acggtggggc acggtaagag aagcgcggat ccgcagggcc gagcatcctg tggaccgtgc
      721 tttcgtctac gcctcaacgg caaggcgttc ctgcacccca gtaacacggc acggacgggc
      781 gggtccgggt caaggaatgt gccactctcc caggtgaagc gatgacaccg ccgaatatct
      841 tgatggtgga ggatgatcct ccgatccgaa ccctgaccgc gcgcgcgctc caggaacatg
      901 gttatatcgt ccgcaccgcc ggcaccgcgc cgcagatgtg ggacgcgctc agggcggcgc
      961 ccgccgacct gttgctgctc gacatcatgc tgcccggcac gtcggggatc gacatctgcc
     1021 gcgaagtccg gcggacgagc gacgtcccga tcatcttcct gtcggcgaag ggaaccgaaa
     1081 ccgaccggat cgttgggctc gagcttggcg ccgacgatta tctcgcgaag ccgttcgggg
     1141 ttcgcgagct ggtcgcgcgc gtgcgcgcgg tgctgcggcg ccatgcgatc gagcgtccgg
     1201 tgacggaccg cgagcgcggc ctgatccgct tcgacaattg gacggccgac ctgccccggc
     1261 gccagctgac ctcgcccgat ggcgcggcgg tcgagctgac cggcgccgaa ttcgacctcc
     1321 tcgtcagcct gtgcgacaat gcacagcgcg tcatcgcgcg cgagcggctc atcgaattgt
     1381 cgcgcacccg cctcggcgac agctctgacc gcagcatcga cgtgctgatc agccgcctgc
     1441 ggcgcaaatt gtcgaacccc ggaaagccgg cgccgatcgt gaccgttcgc ggcatcggct
     1501 atatgctcaa cgtccccgtg gagcggcttt gagcctgttc gaaaagcgcg gcttcggcct
     1561 gttcggacag atcgtcgcga tcctgttcgc cgcggtgctg ctcgaaagcg ccgcgagcat
     1621 ctttctctat gagcgcgcga gccagttttc gatcaacggc gacaaggcgc ggcggcttgc
     1681 cgaacatctc gtcctgtcgg gcaagctgat cgaggaagcg ccgcggtcgc agcgcgacac
     1741 ggtggcgtcc gaactgacga cgacgcgcta tcacctcgta tggcaggaac ggctggtcaa
     1801 tccgccgccg gtgtcgccca aactcaacca gatcacgcgg cagatcctcg actgggagcc
     1861 cgagatgcag gatcgcgacc tgcgcctcta tctccagtcg ccgggactcc acagcacgat
     1921 ttcgggcgcg atccggctga acgacgggag ctgggtcagt ttcagcgcgc gcgatcatgt
     1981 ccgcaaactc gacctgacat tgttccggac tttgcagacg ctggttccgg cgatcgcgct
     2041 cgtgctgctc gccagcctgc tcatccgcca tacgctccgc ccgatgcgcg atttgacgcg
     2101 cgcggccgaa acgctcggca agcgcgattt cgtcgatgtc ccgctgcgcg gcccggcgga
     2161 ggtccggaag gtcatcgacg ctttcaacga catgcagcac cgcatcaccg cgatgatcgc
     2221 cgaccgcacc caggcgctgg cggcggtggg gcatgatttc cgcacgccgc tggcgcggct
     2281 gcggctgcgc gccgacagcg tcgaggatcc gtcggtcggg atcgcgatgg agcaggatat
     2341 cggcgagatg gagcgcatga tcgactcgct gctcgcgtac ctctcgggcg atatcgacga
     2401 tccgacggaa cccgtggcgc cgaccgacat cgcggtgatg tgcgcgaccg ccgccgacga
     2461 agcgagcgac cgggggcatc gcgtccgcta tgccgggccc gaccatctgg tgctgcgcgt
     2521 ccatggcatc gcgatcaagc gcgcgctcct caatctcgtc ggcaatgcgc tgcgcttcgg
     2581 gaacaagata gaggtgcggc tgcgcagcgg cgagcgaaac atcgaaatcg aggtcgccga
     2641 cgacggcccc ggcattcccg aacatctgcg cgaagaagcg gttcagccct ttgcccggct
     2701 cgacagcgcg cgggcgcgcg acaccggcgg tttcggactc ggtctgtcga tcgtcgagcg
     2761 cgttgcccgg atgcacggcg gctcgctcga actgggccag agccatctcg ggggtttggc
     2821 cgcacgcctg cgcctgcccg ccgcctgaga aacatttcgt tacattaacg ctgcaccgca
     2881 gcaaagttcg atcgccatgt gcgtgaaacc aaccagcaag gagtggtttc atgaacgacc
     2941 tcatcggacg cgtgttcgat ttcgaaaagc gcgtcttccc cagccgcaac gacctgttcg
     3001 ccaagctcgt ggccgacggc cagagcccga aggcgctgat gatctcctgc gcggacagcc
     3061 gcgtggcgcc cgaacatgtc atgcaggccg aacccggcga catgttcgtc tgccgcaacg
     3121 ccggcaacat cgtcccgccc tttgcgacgc agaatggcgg cgtgtcgtcg acggtcgaat
     3181 atgccgtcgc cgcgctgggc gtccgcgaca tcatcgtctg cggccattcg gattgcggcg
     3241 cgatgaaggc gctgatgaac ccgcacatga tggaaggcat gcccaatgtc gccgcctggt
     3301 tgcgccacag ccacgccgcc gaacatgtcg tgcgttcggg ctacaccgac ctcgacgatg
     3361 ccggcctggt ccgcgccgcg agcctcgaga atatcgtcgt ccagcttgcg catctgcgca
     3421 cccatccgtc ggtcgcggcg ggtatcgcac gcggtgaaat ttcgctgcac ggctggttcg
     3481 tcgacattca cctcggcgag gtgatgggtc tcgacggcgc cagcgggcag ttcgttccga
     3541 tccgcgccaa cgagcccctg cccgtcgcgc tcgccgccgc gcag
//
LOCUS       DATMSU010000039         5651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_161819,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000039 DATMSU010000000
VERSION     DATMSU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161819"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..676)
                     /locus_tag="VN034_01045"
     CDS             complement(140..676)
                     /locus_tag="VN034_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59212.1"
                     /translation="MLQLSSRVAAPLVFLVGALAVPAAAKEKAPAERPAQIRELYACR
                     DITDTAARLACFDREVGELSAADQAREITFTDRETAKKARRGLFGFSFPKLGGIFGGD
                     EDEVKEIETTIRSVSTDRSGKYTLTMEDDAVWTQIDTTRLPRQPKAGQKVKIKVATMG
                     SYFATVEGGRTIRMKRDR"
     gene            813..2483
                     /locus_tag="VN034_01050"
     CDS             813..2483
                     /locus_tag="VN034_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWV59213.1"
                     /translation="MDSRFSWSTDYHHPTAWDQDYPPLSLPDMLAASVARRGDAPMLD
                     FLGRQFGYAEVARGAARVARGLQQLGLGKGSRVGLFLPNVPHYVAAYYGALMAGATVV
                     NFSPLYTVAELKAQVEDSGTDTLFTLSAAALLPTALKVLDGSSLQRLVVGSVAGGLPA
                     AKSVLYRLFKRSEVAPLPDDPRVIRFSALTDNDGQPDPVALDAQTDIALIQYTGGTTG
                     TPKGAKLTHQNLTANARQVNAIDPDHDAEDRILGVLPFFHVFANTCVLNRTILNGGTI
                     TMLPRFDAKQALEAITRTKTTALPGVPTMYQALLDHPDLATTDFSSLRVCISGGAPMA
                     AELREKFVAATGASLVEGYGLTESSGVVATNPYEGPVRPGTIGQPLPATHIRLLDKED
                     PTKDAPDGEPGELAVKGPQIMQGYWNRPDADKDSFTDDGWLRTGDVAVVEEGGYIRIV
                     DRLKDMIAVGGFKVYPSVIEAHLYEHPAVKEAIVLGVPDAYRGEAPKAFVTLEDGFNV
                     SGEALAAWLNPQLGKHERVNAVDVRLNLPKTMIGKLDRKALRAEEGAA"
     gene            complement(2480..3763)
                     /locus_tag="VN034_01055"
     CDS             complement(2480..3763)
                     /locus_tag="VN034_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HWV59214.1"
                     /translation="MSLLSKPLTLPCGAILPNRIAKAAMTEGMATPDGRPAPELDRLY
                     GLWSDGGAGLLIGGNLVIDRDHLERPGNVVIDGTPDTDMMERLTRWAAAGTRGGNHFW
                     PQISHSGRQTPAIVNQHPKSASDVRLGLPGGQFGQPEPLTRDEIAALVERWAVAAKAC
                     RDAGFTGVQVHAAHGYLMSQFLSPLSNRRTDEYGGSLENRARLLLDVIAAIRAATGPA
                     FPISVKLNSADFQKGGFAFEDSLTVAVWLETAGVDLIEISGGTYEQPKLLGIEGMEES
                     AAQNVAPSTLAREAYFVDFALAMQARLSVPLMVTGGFRTRAAMEQALERGAADVIGLG
                     RPMCLMTDAPKQLLAGAGALPRYEEGLGLVPGWLGFLKRFRIVKALDGFAGIYWFYQQ
                     LWLLGHKGRTDERLSVFSAFRTLEARNKAIMKARG"
     gene            3907..4341
                     /locus_tag="VN034_01060"
     CDS             3907..4341
                     /locus_tag="VN034_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HWV59215.1"
                     /translation="MTHVASPAPVLTRLGALVRAARDSRPLTSLSGDQLAMGLSGLCL
                     VHCLATTIFFASIASVGGVFLDNHLFHEIGLIIAIGFALITLVSGVLSHGYMMPFAVG
                     SFGLGMMAGALSRPHDGSEVIATMIGVAVVALGHDLNRRARH"
     gene            4446..4910
                     /locus_tag="VN034_01065"
     CDS             4446..4910
                     /locus_tag="VN034_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HWV59216.1"
                     /translation="MGKHDHPHVEASGASLAKAAQTALEGAGEAWTDMRAEIFDAVAE
                     IGKPASAYEIADIVSQKRGKRVAPNSVYRILDLFVANNLVRRVESANAFVANSHPGCL
                     HDCIFLICDSCGTAVHVDDDKLSTGVRAAANATGFAAERPVIEVRGKCAECQ"
     gene            4945..>5651
                     /locus_tag="VN034_01070"
     CDS             4945..>5651
                     /locus_tag="VN034_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59217.1"
                     /translation="MFGGLQHWIEAQRARPPAPTRAWTWTLAFGIATLLFGLHLTQVF
                     PQTGHDIAPGYGAPVLAFEFAGSQADLEAIFGFFTDPQQVTRLAAMRTGNERDYLYML
                     LYAGFLVSGCIALWRELRHRALLAAAALPVAAALCDAWENWLLFDIQAAFTLGDYSPA
                     MASLPYPVAAKFLFLAATNVMIGAAATQLGRWWALFGTLAILAAIPTAMAIVTPAAFA
                     WALIPSAAGGWLLLLALA"
BASE COUNT          915 a         1984 c         1807 g          945 t
ORIGIN      
        1 gaacagtcgt gctgccgcgg ctccctcggc acgcgcgtcg tcgagcagca cgaagggcgg
       61 gcagcccggt cccggaacgg ggatcgcgtc gccgcccgcc atcgctaatt cacggagatc
      121 tgtcgcgcgc cggcagcaat cagcggtcgc gcttcatgcg gatcgtacgg ccgccttcga
      181 ccgtggcgaa atagctgccc atcgtcgcga ccttgatctt gaccttctgc cccgccttgg
      241 gctggcgcgg cagccgcgtc gtgtcgatct gcgtccacac cgcgtcgtct tccatcgtca
      301 gcgtatattt gccggaccgg tccgtgctga ccgaacggat cgtcgtttcg atttccttga
      361 cctcatcctc atcgccgccg aaaattccgc cgagcttggg aaaggaaaag ccgaaaagcc
      421 cgcgccgcgc ttttttggcg gtctcgcggt cggtaaaggt gatctcgcgc gcctgatcgg
      481 cagccgacaa ttcgcccact tcgcggtcga aacaggcgag ccgcgccgcc gtatcggtga
      541 tgtcgcggca cgcgtagagt tcgcggatct gcgccgggcg ttcggcgggc gctttttcct
      601 tcgcggccgc cggaacagcg agcgcgccga caagaaagac gagcggcgcg gcgacgcgcg
      661 atgacagctg cagcatggca aaacccctgg cgagaacgat atgcgctgcc tagccgcagc
      721 ggcgccgcgc cgcaagcgag cccggttgcg gtcgcggctt actgacccta gtgtaaggaa
      781 gccgaaccat attcgagagg atcccgcccg cgatggacag ccgcttttcc tggtcgaccg
      841 attatcacca cccgacggca tgggatcagg attatccgcc gctgtcgctg cccgatatgc
      901 tcgccgccag cgtggcgcgg cgcggcgatg cgccgatgct cgactttctg ggccgccagt
      961 tcggctatgc cgaggtcgcg cgcggcgcgg cacgcgtcgc gcgcgggctt cagcagctgg
     1021 ggctcggcaa gggcagccgc gtcgggctct tccttcccaa cgtcccccat tatgtcgccg
     1081 cctattatgg cgcgctgatg gcgggcgcga ccgtcgtcaa cttctcgccg ctctataccg
     1141 tcgccgagct caaggcgcag gtcgaggatt cgggcaccga cacgctcttc acgctgagcg
     1201 ccgccgcgct cctccccacc gcgctcaaag tgctcgacgg atcgagcctc cagcgcctcg
     1261 tcgtcgggtc ggtcgcgggc ggattgccgg ccgcgaaatc ggtactctac cgcctgttca
     1321 agcgcagcga ggtcgcgccg ctgcccgacg acccgcgcgt catccgcttt tccgccctca
     1381 ccgacaatga cggccagccc gaccccgtcg cgctcgacgc ccagaccgac atcgcgctga
     1441 tccaatatac cggcggcacc acgggaacgc cgaagggcgc caagctgacc caccagaatc
     1501 tcaccgccaa cgcgcggcag gtgaatgcga tcgaccctga ccatgatgcc gaggaccgca
     1561 tcctcggcgt gctccctttc ttccacgtct tcgcgaatac ctgcgtgctc aaccgcacga
     1621 tactcaacgg cggcacgatc acgatgctgc cgcgcttcga cgcgaagcag gcgctcgagg
     1681 cgatcacgcg gacgaaaacg accgcgctgc ccggcgtgcc gacgatgtat caggcgctgc
     1741 tcgaccatcc cgatctggca acgaccgatt tctcgtcgct gcgcgtctgc atctcgggcg
     1801 gcgcgccgat ggccgccgaa ctgcgcgaga aattcgtcgc cgcgaccggc gcctcgctcg
     1861 tcgagggcta tggcctcacc gaaagctcgg gggtcgtcgc gaccaacccc tatgaaggac
     1921 cggtgcgacc cggcacgatc ggccagccgc tccccgcgac gcacatccgc ctgctcgaca
     1981 aggaagaccc gaccaaggac gcccccgacg gcgagcccgg cgagctcgcg gtcaaggggc
     2041 cgcagatcat gcagggttac tggaaccgcc ccgacgcgga caaggacagc ttcaccgacg
     2101 acggctggct gcgcaccggc gatgtcgcgg tggtcgagga gggcggctat atccgcatcg
     2161 tcgaccggct gaaggacatg atcgcggtcg gcggattcaa ggtctatccg agcgtgatcg
     2221 aagcgcatct ctatgaacat cccgcggtca aggaagcgat cgtgctcggc gttcccgacg
     2281 cctatcgcgg cgaggcgccg aaagcctttg tgacgctcga ggacggtttc aacgttagcg
     2341 gcgaggcgct cgccgcctgg ctcaaccccc agctcggcaa gcatgagcgg gtgaatgcgg
     2401 tggacgtgcg gttgaacctg cccaagacga tgatcggcaa gctcgaccgc aaggcgctcc
     2461 gcgcggaaga gggcgcggcc taaccccgcg ccttcatgat cgccttgttg cgcgcctcga
     2521 gcgtgcggaa ggccgaaaag acgctcagcc tttcatccgt ccggcccttg tggccgagca
     2581 gccagagctg ctggtagaac cagtagatgc cagcaaaacc gtcgagcgcc ttgacgatcc
     2641 ggaagcgttt cagaaagccc agccagccgg gaacgagccc cagcccctcc tcgtaacgcg
     2701 gcaacgcccc ggcgcccgcg agcaactgct tcggcgcgtc ggtcatcagg cacatcgggc
     2761 ggccgagccc gatcacgtcg gcggcgccgc gctcgagcgc ctgttccatc gcggcgcgcg
     2821 tccggaaacc gcccgtcacc atcagcggca ccgacaggcg cgcctgcatc gcgagcgcga
     2881 aatcgacgaa ataggcctca cgcgcgagcg tcgagggcgc gacgttctgc gcggcgcttt
     2941 cctccatgcc ctcgatcccg agcagtttgg gctgctcata agtgccgccc gaaatctcga
     3001 tcagatcgac gcccgcggtt tcgagccata cggcgacggt caagctgtcc tcgaaagcga
     3061 aaccgccttt ctggaaatcg gcgctgttga gcttgaccga aatcgggaag gccggcccgg
     3121 tcgccgcgcg gatggcggcg atcacgtcga ggagcaggcg ggcgcgattt tcgaggctgc
     3181 cgccatattc gtcggttcgc cggttcgaca gcgggctcag gaactgcgac atcagatagc
     3241 cgtgcgcggc atgcacctgc accccggtga agccggcgtc ccggcacgcc ttcgccgcca
     3301 ccgcccagcg ttccacaaga gcggcgatct cgtcccgggt cagcggctcg ggctggccga
     3361 actgcccgcc aggaagcccc agccggacgt ccgacgccga tttggggtgc tggttgacga
     3421 tcgccggggt ctgccgcccg ctgtggctga tctgtggcca gaaatgattg ccgccgcgcg
     3481 ttccggctgc ggcccagcgc gtcagccgtt ccatcatgtc ggtgtcgggc gtcccgtcga
     3541 tcacgacgtt gcccgggcgt tcgagatggt cgcggtcgat caccagattg ccgccgatca
     3601 gcagccccgc gccgccatcg gaccacagac catagagccg gtcgagctcg ggcgccggcc
     3661 ggccgtcggg ggtcgccatc ccctcggtca tcgccgcctt ggcgatccgg ttcggcagga
     3721 tcgccccgca cggcaatgtc agcggcttcg acaaaaggct cataaccgtc cctccccacc
     3781 cggttttcct cgcgaaacgg actgatcccg ccctcgggcc aagtcaaaaa gattcgcgta
     3841 tgtgatattg taaccttggt catgttatgc catatcaggc gccgtaatcg ttgaacggag
     3901 aatcgggtga cccatgtcgc cagccctgcc cctgtcctga cgcgcctcgg cgcgctggtc
     3961 cgggctgcgc gcgattcacg gccgctgacc tcgctgtcgg gcgatcagct tgcgatggga
     4021 ttgtcggggc tctgcctcgt ccactgcctc gcgacgacga tctttttcgc ctcgatcgcc
     4081 tcggtcggcg gggtgtttct cgacaatcat ttgttccatg agatcgggct gatcatcgcg
     4141 atcggcttcg cgctgatcac gcttgtctcg ggcgtattga gccacggcta tatgatgccg
     4201 ttcgccgtcg gcagcttcgg gctcggcatg atggcgggcg cgctgtcgcg gccgcacgac
     4261 ggcagcgaag tgatcgcgac gatgatcggc gtggcggtgg tcgctctggg tcacgacctc
     4321 aaccgccgcg cgcggcactg acgcgtccgc cgaaacggac cggtccgcgg cttgcgggcg
     4381 ccccccaaag agcctacatt ggaagcgtag ttagcgcgga attatccgcg catgaggaaa
     4441 cgccaatggg caagcatgat catccgcacg tcgaggccag cggcgcctcg ctcgccaagg
     4501 cggcgcagac cgcactcgag ggcgcgggcg aagcctggac cgacatgcgc gccgagattt
     4561 tcgacgcggt cgccgaaatc ggcaaacccg cgagcgccta tgaaatcgcc gacatcgtct
     4621 cgcagaagcg cggcaagcgc gtcgcgccga acagcgtcta tcgcatcctc gacctgttcg
     4681 tcgcgaacaa cctcgtccgc cgggtcgaga gcgcgaacgc ctttgtcgcg aacagccatc
     4741 cgggctgcct ccacgactgc atcttcctga tctgcgacag ctgcggcacc gcggtgcatg
     4801 tcgacgacga caagctatcg accggggtcc gcgccgccgc gaacgccacc ggcttcgccg
     4861 ccgagcgccc cgtcatcgaa gtgcgcggca aatgtgccga gtgccaataa agtgagccgc
     4921 cgcgctccac cggacggcta gagcatgttc ggggggcttc agcattggat cgaggcgcag
     4981 cgcgcccgcc cgcccgcgcc gacgcgcgcg tggacctgga cgctggcgtt cgggatcgcg
     5041 accctgctct tcggcctcca tctgacacag gtctttccgc aaacgggcca tgacatcgcg
     5101 ccgggctatg gcgcgccggt gctcgcgttc gaattcgcgg gcagccaagc cgacctcgaa
     5161 gcgatcttcg gcttcttcac cgacccgcag caggtgacgc gcctcgccgc gatgcgcacc
     5221 ggcaacgagc gcgattatct ctatatgctg ctctatgcgg gctttctcgt cagcggctgc
     5281 atcgccttgt ggcgcgagct ccggcatcgc gcgctgctcg ccgccgcggc gcttccggtc
     5341 gccgcggcgc tgtgcgacgc atgggagaat tggctgctct tcgacatcca ggccgccttc
     5401 acgctcggcg attattcgcc cgcgatggcg agccttccct atcccgttgc ggcgaaattc
     5461 ctgttcctcg ccgcgaccaa cgtcatgatc ggcgcggcgg cgacgcagct cgggcgctgg
     5521 tgggcgctct tcggcacgct cgcgatcctc gccgcgatcc cgaccgcgat ggcgatcgtc
     5581 acccccgcgg ctttcgcttg ggcgctgatt ccctcggcgg cgggcggctg gctgctgctt
     5641 ctcgcgctgg c
//
LOCUS       DATMSU010000040         5674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_86196, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000040 DATMSU010000000
VERSION     DATMSU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86196"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VN034_01075"
     CDS             <1..195
                     /locus_tag="VN034_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59218.1"
                     /translation="PRPDLVPERRARIAALVAAHAAGDKNAHVTAATALAAEFAQDAS
                     ILFLIERLNETEKGESYVLS"
     gene            182..940
                     /locus_tag="VN034_01080"
     CDS             182..940
                     /locus_tag="VN034_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59219.1"
                     /translation="MSFPDFPMRRFGLTAAAAIAAMTIVTAAAAQSMVVRSTGPSAAK
                     YPAGARLKPADKLTLVAGDRIVLMQSGKTRTLSGPGTFAATGTVQASQSLGGNVSRML
                     AKGPTMRSRGGFSRGPDDPIPAEHRAPNLWLLDYREGGTFCVLDPALLMLWRPNMQGD
                     TALEIESGGKKTTVAIVDGANFRKWPTDVLPVQYGADYRLSGGGLAQPVTVRFTPVEN
                     APDTVEGSVDMLMAKGCTPQLNRLVDTMSDAEAG"
     gene            1118..2941
                     /locus_tag="VN034_01085"
     CDS             1118..2941
                     /locus_tag="VN034_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HWV59220.1"
                     /translation="MAVSGDMGFHGAARPKYAALRRALAGSAGLIAAALPAPLWAQAT
                     PQIPTREEIQRPAIPPAARPSEQIVAIDDGIERAPCPLANPEFAHIRFTLRGVEFSAV
                     EGIDNALLAPSWSDRVGQELPISAVCDIRDRAATILRSQGYLAAVRVPPQTIGDGIVR
                     LDILSARMTRIEVRGDAGANEKLLQRYLSRLDDAPVFNIADAERYLLLARDIPGMDAR
                     LTLRPGTVAGEVVGEVTVTRTPVTFDFNVQNLGSRDVGRWGGNARARFAGLTGMGDLT
                     TVSFYATPDLDEQKVVQASHEFRVGGEGLRLGASYTYAWTRPDLTGLSIKSNTQIVSL
                     FGSYPLVLTQARRVTIGGGLDIIDQDISLTGVPINEDRLRVFNLRGDASWIDPASIAG
                     RGGYNPAEPRWSLATSLEARQGIDFLGASDDCGPGGTACFLPGAVPLTRIEGKPDAFL
                     VRANALAEWRPAKLFTLSAAPRAQWANDPLLAYEEFSGGNFTVGRGFDPGTVIGDSGV
                     AVALEARYGSFVPASTRAFAFQPFVFFDAAWVWNEDAAFDGLDPQRLYSAGGGVRVAY
                     GNLGRLDVTLAVPLNRGGFLTERPDPRLLVSLTTQFGVGAR"
     gene            2971..>5674
                     /locus_tag="VN034_01090"
     CDS             2971..>5674
                     /locus_tag="VN034_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59221.1"
                     /translation="MRATATFSPVRKGKRHLLTSCAIAAGIAALAYGGPASAQVAGSG
                     SFVSGTGSIGNDGATNSTTVNVDGAQSIINWVPTDTAPTGGAIDLLPAGENWNFVGTG
                     DYTVLNRVVNGSGGSLSRQIALNGTVNSYTGSTSGPRGGNIWFYNAGGILIGASGVIN
                     VGSLLLTTNDIVTPGGLFGPGGTIRFNGASGSTSAITVNGAINANITGSPGSSYVALV
                     APRVVQAGAVRVDGSAAYVAAEQANIRINGGLFDIDVTVGAEGGNAITHTGTTTGPAH
                     LDTNGNQSRIYMVAIPKNDAVTMLVSGAIGYDDALSAQVDPDGAVRLSAGYNIVGGEL
                     AATPTNTTAANITAGDTLLRSNTVARASGAFAATPTQVLPGSSPPLSSAGQFFVEGSG
                     TFIGDASATVSIGANQSGGATGSFTVQSGGRGGTPGNAAVNVSGGTLSAPGALTIRAS
                     GTPDAVTGDAQGGTASLTITGGAVSSQGVTVEANGFGDLDAGGSSGNGRGGSASISVS
                     NAGSTLTAGNIFINAVGSGDNSFSTVPAVNGSGTGGTASLTVANGGSVGPAALLSLDA
                     SGFGGFGLNQSGDGTGGTARITVSGAGSFLSATQSFVDATGEGGGNFGSFLTRNGGNG
                     TGGTAEILINADATSVVSLGNLGAFGFGEGGNAAGSENTTGGDALGGSARLIVEGGVT
                     AQFTQIVLDASAAAGTATTPSGTTGQSGNARANNVTLTATNGSTIDSSGLINLFAVGM
                     NPYPFNPAGENFGAGTGGNIAVNATAGGTITTGNSLTAQAMGGSMSDTIRQSAGDGIG
                     GNVDFLADGGTIQAEVYDVDASGNTANVTGTGGTAQGGTIDVFAQNGGQFIATLDATN
                     RFDASATTGISAAGTAATGGTIQLIADAGTIDLGIGANLFAEGSS"
BASE COUNT          937 a         1995 c         1884 g          858 t
ORIGIN      
        1 ccgcgccccg atcttgtccc ggaacggcgc gcccgcatcg ccgcgcttgt cgcggcgcac
       61 gcggcgggcg ataaaaatgc ccatgtgacc gctgccacag cattggccgc ggaattcgcc
      121 caggacgctt ccatcctgtt cctgatcgaa cgcctgaacg agaccgaaaa gggagaaagc
      181 tatgtccttt cctgacttcc cgatgcgccg cttcggcctg accgccgccg ccgccatcgc
      241 cgccatgacc atcgtcaccg ccgccgcggc gcaatcgatg gtcgtccgct cgaccggccc
      301 gtcggccgcc aaatatccgg ccggcgcgcg gctcaaaccg gccgacaagc tgaccctcgt
      361 cgccggcgac aggatcgtgc tgatgcagtc gggaaagacg cgcacgcttt ccggtcccgg
      421 cacctttgcc gccaccggta cggtgcaggc aagccagtcg ctcggcggca atgtcagccg
      481 gatgctcgcc aaggggccga cgatgcggtc gcgcggcgga ttttcgcgcg gccccgacga
      541 tccgatcccc gccgagcatc gcgcgcccaa tctctggctg ctcgactatc gcgagggcgg
      601 gaccttttgc gtcctcgatc ccgcgttgct gatgctgtgg cgccccaata tgcaaggcga
      661 taccgcgctc gagatcgaaa gcggcggcaa gaagacgacc gtcgcgatcg tcgatggcgc
      721 caatttccgc aaatggccga ccgatgttct gccggtgcaa tatggcgccg actatcgcct
      781 ctcgggcggt ggcctcgcgc agccggtcac ggtgcgcttc acccccgtgg aaaacgcccc
      841 cgacacggtc gaggggtcgg tcgacatgct gatggcgaag ggttgcacgc cgcagctcaa
      901 ccgcctcgtc gatacgatgt cggacgcgga ggcgggttga gcagagggac aggccgcgat
      961 tgaacgcgtc ctatccgtct gatattattc ccgggaaata ccgcctgtcg cgtggcggaa
     1021 ccttccctgg gtcgaaattc gctgcgccgg gctaccggcg gagccaatga aatggacgcc
     1081 cccgcgatcg gggtgcgccc gaacgcaagg ggtggtgatg gccgtgtcgg gggatatggg
     1141 atttcatggg gctgcgaggc cgaaatacgc ggcgcttcgc agggcactcg cgggctcggc
     1201 ggggttgatc gcggccgcgc tgcccgcccc cctgtgggcg caggcgacgc cgcaaatccc
     1261 gacgcgcgag gaaatccagc gtccggcgat acctcccgct gcgcggccca gcgaacagat
     1321 cgtcgcgatc gacgacggca tcgaacgcgc gccctgcccg ctcgccaatc ccgaattcgc
     1381 ccatatccgc ttcacgctgc gcggggtgga attttcggcc gtcgaaggca tcgacaacgc
     1441 cctgctcgcg ccgagctggt cggatcgcgt cgggcaggag ctcccgattt ccgcggtgtg
     1501 cgatattcgc gaccgcgccg cgacgatcct gcggtcgcaa ggctatctcg ccgcggtccg
     1561 cgtgccgccg cagacgatcg gcgacggcat cgtccggctc gacatccttt cggcgcggat
     1621 gacgcgcatc gaggtgcgcg gcgatgcggg cgccaacgag aagctgctgc aacgctatct
     1681 ctcgcggctt gacgatgcgc cggtgttcaa tatcgccgat gccgaacgct atctgctgct
     1741 tgcgcgcgac atcccgggca tggacgcgcg gctgacgctg cgccccggca cggttgccgg
     1801 cgaagtcgtc ggcgaagtga ccgtcacgcg cacgcccgtc accttcgact tcaatgtgca
     1861 gaatctgggg tcgcgcgacg tcggccgctg gggcggcaac gcgcgcgcgc gcttcgcggg
     1921 cctgaccggc atgggcgatc tcaccacggt cagcttctac gcgacccccg atctcgacga
     1981 acaaaaggtc gtgcaggcgt cgcacgagtt ccgcgtcggc ggcgaagggc tgcgcctcgg
     2041 cgccagctat acctacgcct ggacgcgccc cgacctcacc ggtctctcca tcaagtcgaa
     2101 cacccagatc gtcagcctgt tcgggtcata cccgctcgtc ctgacgcagg cgcggcgcgt
     2161 cacgatcggc ggcgggctcg acatcatcga tcaggacatc agcctgaccg gcgttccgat
     2221 caacgaggac cggctgcgcg tcttcaacct gcgcggcgac gccagctgga tcgaccccgc
     2281 ctcgatcgcc gggcgcggcg gatataatcc cgccgaaccg cgctggtcgc tcgcgacgtc
     2341 gctcgaggcg cgccaaggta tcgatttcct cggcgccagc gacgattgcg gccccggcgg
     2401 cacggcctgc ttcctgcccg gcgcggtgcc gctgacgcgc atcgagggca agcccgacgc
     2461 ctttctggtg cgcgccaacg cgctcgccga atggcggccg gcaaagctct tcacgctgtc
     2521 ggcggcgccg cgcgcgcaat gggcgaacga tccgctgctc gcctatgagg aattttcggg
     2581 cggcaatttc accgtcgggc gcggcttcga tcccggcacg gtgatcggcg acagcggcgt
     2641 cgcggtggcg ctcgaggcgc gctacggctc gttcgtcccc gcgagcacgc gggccttcgc
     2701 cttccagccc ttcgtctttt tcgacgccgc ctgggtgtgg aacgaggatg ccgccttcga
     2761 cgggctcgat ccgcaaagac tctattcggc gggcggcggt gtgcgcgtcg cttatggcaa
     2821 cctcggccgg ctcgacgtca cgctcgccgt gccgctcaat cgcggcggct tcctcaccga
     2881 acggcccgac ccgcggctgc tcgtgtcgct gaccacccaa ttcggcgtgg gggcgcgctg
     2941 accatgacct ttgcttcgag gacgactgcc atgcgtgcca ccgccacctt ctcgcccgtc
     3001 cgcaaaggca agcggcacct gctgaccagt tgcgcgatcg cggccggcat cgccgcgctt
     3061 gcctatggcg gcccggcttc ggcgcaagtg gccggttcgg gcagcttcgt atcgggcacc
     3121 ggctcgatcg gcaacgatgg cgcgaccaac tcgacgacgg tgaacgtcga cggggcgcag
     3181 agcatcatca actgggtgcc gaccgacacc gcgccgacgg gcggcgcgat cgacctgctc
     3241 cccgcgggcg agaactggaa tttcgtcggc accggcgact atacggtcct caaccgcgtc
     3301 gtgaacggca gcgggggatc gctcagccgc cagatcgcgc tcaacggcac ggtgaactcc
     3361 tataccggat cgaccagcgg cccacgcggc ggtaatatct ggttctacaa cgccggcggc
     3421 atcctgatcg gtgcgagcgg ggtcatcaac gtcggcagcc tgctgctcac gacgaacgac
     3481 atcgtcaccc ccggggggct gtttggcccc ggcggcacga tccgcttcaa tggcgcgtcg
     3541 ggcagcacct cggcgatcac cgtcaacggg gcgatcaacg ccaatatcac cggcagtccc
     3601 ggcagctcct atgtcgcgct ggtcgcgccg cgcgtcgtgc aggccggcgc cgtccgcgtg
     3661 gacggatcgg ccgcctatgt cgccgccgaa caggcgaata tccgcatcaa cggcggcctc
     3721 ttcgacattg atgtcacggt cggcgcagag ggcggcaacg cgatcaccca caccggcacg
     3781 accaccggcc cggcgcatct cgacaccaac ggtaaccaga gccgcatcta tatggtcgcc
     3841 attcccaaga atgacgcggt gacgatgctg gtcagcgggg caatcggtta tgacgacgcg
     3901 ctgtcggcgc aggtcgatcc cgacggcgcg gtacgcctgt cggcgggtta caacatcgtc
     3961 ggcggcgaat tggcggcaac gccgaccaat acgaccgccg cgaacatcac cgccggcgat
     4021 acgctgctgc gtagcaacac ggtcgcgcgc gcaagcggcg cctttgccgc cacgccgacg
     4081 caggtgctgc cgggatcgtc cccgccgctg tcgtcggcgg gccagttctt cgtcgagggc
     4141 agcgggacct tcatcggcga cgccagcgcg acggtttcga tcggcgccaa tcagtcgggc
     4201 ggcgcgacgg gaagcttcac ggtccagtcg ggtggccgcg gcggcacgcc cggcaacgct
     4261 gcggtaaacg tctccggagg gacactctcg gcgcccggcg cgctcacgat ccgggcttcg
     4321 ggcacgccgg acgcggtcac cggcgatgcg caaggcggca ccgccagtct gacgatcacc
     4381 ggcggcgccg taagctccca aggcgtcacg gtcgaagcca atggcttcgg cgacctcgac
     4441 gcgggcggct caagcggcaa cgggcgcggg ggcagcgcca gcatttcggt cagcaatgcg
     4501 ggcagcacgc tgaccgcggg caatatcttc atcaacgcgg tcgggagcgg ggataacagc
     4561 ttcagcaccg tccccgctgt caacggcagc gggaccggcg gcacggccag cctgacggtc
     4621 gccaacggcg gcagcgtcgg gccggcagcc ctgctttcgc tcgacgcaag cgggtttggc
     4681 ggcttcggcc tcaaccagtc gggcgacggc accggcggta ccgcacgcat caccgtgtcg
     4741 ggcgcgggct ccttcctctc ggcgacgcaa agcttcgtcg acgcgacggg cgaaggcggc
     4801 gggaatttcg gcagctttct gacccggaat ggcggcaacg ggacgggcgg cacggccgaa
     4861 atactgatca acgccgatgc gacctcggtc gtgtcgctcg gcaacctcgg cgccttcggc
     4921 ttcggcgagg gcggcaacgc cgccggaagc gagaatacaa ccggcggcga cgcgctgggg
     4981 ggttcggccc gtttgatcgt cgaaggcggc gtcaccgcgc aattcaccca aatcgtgctc
     5041 gatgcgtccg cggctgccgg aacggccacc acccccagcg gcacgaccgg ccagtcgggc
     5101 aacgcgcgcg cgaacaatgt gacattgacc gcgacgaacg ggtcaacgat cgacagcagc
     5161 ggcctcatca acctcttcgc cgtcggcatg aatccctatc cgttcaaccc cgccggcgaa
     5221 aatttcggcg caggcacggg cggcaatatc gcggtcaacg cgaccgccgg gggcacgatt
     5281 accaccggca acagtctcac cgcgcaggcg atgggcggca gcatgtccga caccattcgg
     5341 caaagcgccg gtgacggcat cggcggcaat gtcgattttc tcgccgacgg cggaacgatc
     5401 caggccgaag tctatgacgt cgatgccagc ggcaacaccg ccaatgtcac cggaaccggc
     5461 ggcacggcgc agggcggcac gatcgacgtc ttcgcccaaa atggcggaca atttatcgca
     5521 acgctcgacg cgaccaaccg cttcgacgca agcgccacga cgggtatcag cgccgcgggg
     5581 acggcggcta ccggcggaac gatccagctg atcgccgacg ccggcacgat cgacctcggc
     5641 atcggcgcca acttgttcgc cgaaggcagc tcgg
//
LOCUS       DATMSU010000041         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_38907, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000041 DATMSU010000000
VERSION     DATMSU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38907"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..3155
                     /locus_tag="VN034_01095"
     CDS             285..3155
                     /locus_tag="VN034_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59222.1"
                     /translation="MNSRNQFRAHTLRAALLAGAGTTLLVAAMPAMAQDAPAPQAEGE
                     EDAIVVTGIRETIQNSINTKREETAIVDALSADDIGDLPALSVGQAIQTITGATTHRE
                     KGDASEIALRGLGPFLSNATFNGRDATNGSGDRSVNFNQFPSELVNNIKIYKTQQASL
                     IEGGVAGTIEIGTLRPLDFGKRRIQGEIKAQYNPYGDRIVGSGGIGWRGTLSYVDQFA
                     NDTIGIAIGVQRNDTNNPEETYAASTTWTACQADIVVANNNCNEYTRDEYGEDHPFYL
                     VPNAYTFRQISEVDKRDAIFGAIQWRPSDQFNINLDVQYSDRTFVESRRDLNLSEARR
                     SLTDVVYDENGIVQSLSGQSALESNGSELSRAEEYLGGGLSVEWQPNDRLTLTLDGSY
                     SRTIRVEDERNVRLRTDQRDLNGNLNFFAGPQNNNIRVPYTYEITPGSFVPTITIDPR
                     FDLNNHDLFWDDARFRRDQSRRHNEIIAGRFDAGYEMDGFLSKLSAGVRWSKMTFRDY
                     DVRNEDFDLTSDIDEERRINNLCRNRAFPQTGFLSAADGNGINSWATFDVDCLFREYT
                     GSEDPGALADKRSPANRDVTERTLAGYIMADYDADLGNMPVRGNIGVRVVRTDVTSNG
                     LRSELTIIDNGDGTIRLDTTGDFETVTIKSSNTRILPSVNAIFEVAPNTLLRVAGYRA
                     MSRPAPSALGAGRTFQIDADDFTSVEEAIGNVRANGSPRLKPLMSWNADAAIEWYPNK
                     DSLLSATVYYKQFNGGFQPVVFDEEFVIDGQAVTVPVTQTRNSPDKSRIYGLELTAAT
                     RFSFLPKPLDGLGAKVSYNYADSNFENQDVNLGDQYDPETETVSPGIIPAAGLSGYSK
                     HVLSAQAYYEIGPVSLQAIYNYRSKYYQDFVGGNSQLRYVGPSETVDFRASMDLMPGV
                     SLRFEALNLFNEPKATYMPVYGSSRQYHYYGSKYFIGLRARI"
BASE COUNT          675 a         1057 c          923 g          523 t
ORIGIN      
        1 cggcaccgcc tccgccgccg ccgatcgcgg gcgaaggcgc gcgcggacag gtgacgcgca
       61 agttcgacta cacgcacctc ggcaacgtcg gcgccgaagt caccgccaag ctggtggccg
      121 aggcgcttgc ccgggcagtg cccgggatca ggagccagct tgtccgctga caaatcgtgt
      181 atggcaaaat ggactaacca atctgccata cacaatggtt gacaatcatg ctgcgatgtt
      241 cgacagcatt ggtcataaca gaatcggtat ttgggagaga cgaagtgaat tctcgtaatc
      301 agttccgcgc gcacacgctc cgcgccgcct tgctggccgg tgcgggcacg acgttgttgg
      361 tcgccgcgat gcccgcgatg gcgcaggacg cgccggcgcc gcaggccgaa ggcgaggaag
      421 acgcgatcgt cgtcaccggg atccgcgaga cgatccagaa ttcgatcaac accaagcgcg
      481 aagagaccgc gatcgtcgat gcgctgtcgg ccgacgacat cggcgacctt cccgcgctgt
      541 cggtcggtca ggcgatccag acgatcaccg gcgcgacgac gcaccgtgaa aagggcgacg
      601 cctccgaaat cgcgctgcgt ggcctcggtc ccttcctctc gaacgccacc ttcaacggcc
      661 gcgacgcgac caacggcagc ggcgaccgct cggtcaattt caaccagttc ccgtccgaac
      721 tcgtcaacaa tatcaagatc tacaagaccc agcaggcgag cctgatcgaa ggcggcgtcg
      781 ccggcacgat cgaaatcggc acgctacgcc cgctcgactt cggcaagcgc cgcatccagg
      841 gcgagatcaa ggcgcaatac aacccctatg gcgaccgcat cgtcgggtcg gggggcatcg
      901 gctggcgcgg gacgttgagc tatgtcgacc agttcgcgaa cgatacgatc ggcatcgcga
      961 tcggcgtcca gcgcaacgac accaacaatc cggaagagac ctacgccgcc tcgaccacct
     1021 ggacggcgtg ccaggccgac atcgtcgtcg cgaacaacaa ttgcaacgaa tatacccgcg
     1081 acgaatatgg cgaggaccac cccttctacc tcgtcccgaa cgcctatacg ttccgccaga
     1141 tcagcgaagt cgacaagcgc gacgcgatct tcggcgcgat ccagtggcgg ccgtcggacc
     1201 agttcaacat caatctcgac gtccaatatt cggaccgcac cttcgtcgag agccgccgcg
     1261 atctcaatct gtccgaagcg cgccgcagcc tgaccgacgt cgtctatgac gaaaacggca
     1321 tcgtgcagag cctgagcgga cagagcgcgc tcgaatcgaa cgggtccgaa ctgtcgcgcg
     1381 ccgaggaata tctgggcggc ggcctgtcgg tcgaatggca gccgaacgac cggctgacgc
     1441 tgaccttgga cggcagctat tcgcgcacga tccgcgtcga ggacgaacgc aacgttcgcc
     1501 tgcgcacgga tcagcgggac ctcaatggca accttaactt cttcgccggt ccccagaaca
     1561 ataatatccg tgttccctat acttacgaga tcacgccggg cagcttcgtc ccgacgatca
     1621 cgatcgatcc gcgtttcgac ctcaacaatc acgacctctt ctgggacgat gcgcgcttcc
     1681 gccgggacca gagccgccgg cataatgaga tcatcgccgg ccgcttcgac gccggatatg
     1741 aaatggacgg cttcctgagc aagctgtcgg ccggcgtccg ctggtcgaaa atgaccttcc
     1801 gcgactatga cgtgcgcaac gaggacttcg acctcacctc cgacatcgac gaagagcgcc
     1861 ggatcaataa tctgtgccgc aaccgggcct tcccgcagac cggcttcctg tcggccgccg
     1921 acggcaacgg tatcaacagc tgggcgacct tcgacgtcga ctgcctgttc cgcgaatata
     1981 ccggcagcga agaccccggc gcgctcgcgg acaaacgctc gccggcaaat cgcgacgtga
     2041 ccgaacgcac cctcgcgggc tatatcatgg ccgattacga cgccgacctc ggcaacatgc
     2101 ccgtgcgcgg aaacatcggc gtgcgcgtcg tcaggaccga cgtcacttcg aacggcctgc
     2161 gcagcgaact caccatcatc gacaatggcg acggcacgat ccgcctcgat accaccggcg
     2221 atttcgaaac ggtgacgatc aagagcagca acacgcgcat cctgcccagc gtcaacgcga
     2281 tctttgaagt cgcaccgaac acgctgctgc gcgtcgcggg ctatcgcgcg atgtcgcgcc
     2341 ccgcgccgag cgcgctcggc gccggccgca ccttccagat cgacgccgac gacttcacct
     2401 cggtcgagga agcgatcggt aacgtccgcg ccaacggcag cccgcgcctg aagccgctga
     2461 tgtcgtggaa cgccgatgcc gcgatcgaat ggtatccgaa caaggacagc ctcctctcgg
     2521 caaccgtcta ttacaagcaa ttcaacggcg gcttccagcc cgtggtgttc gacgaggagt
     2581 tcgtcatcga cggacaggcg gtgaccgtgc cggtgacgca gacgcgcaac agccccgaca
     2641 agtcgcgcat ctacggcttg gaactcaccg ccgcgacgcg cttcagcttc ctgcccaagc
     2701 cgctcgacgg tctcggcgcg aaagtcagct acaattacgc cgattccaat ttcgagaatc
     2761 aggacgtcaa cctcggcgac caatatgatc ccgaaaccga aaccgtgtcg ccgggcatca
     2821 tccctgcggc gggtctgtcg ggctattcaa agcatgtgct gtcggcgcag gcctattacg
     2881 agatcggccc ggtgtcgctg caggcgatct acaattaccg gtcgaaatat tatcaggact
     2941 tcgtcggcgg caacagccag ttgcgctatg tcggacccag cgaaacggtc gatttccgtg
     3001 cttcgatgga tctgatgccc ggcgtgtcgc tgcggttcga ggcgctcaac ctcttcaacg
     3061 aacccaaggc gacttatatg ccggtctatg gaagcagccg acaatatcat tattacggat
     3121 ccaaatattt catcggcctc cgggcgcgga tctaggcggc tggaaactaa agttcggt
//
LOCUS       DATMSU010000042         1989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_171517,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000042 DATMSU010000000
VERSION     DATMSU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171517"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..906)
                     /locus_tag="VN034_01100"
     CDS             complement(307..906)
                     /locus_tag="VN034_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HWV59223.1"
                     /translation="MPVRTLFATNFYEADIGTPELLEELEESSRLFAEEDGAGRRWSR
                     EHGYKGYTSYASLGDLPERDPAFHDLKRLLDKEVKAFAKACHFDLAKPLKLDSLWVNI
                     LKPGGTHSGHIHPHSIVSGTFYVAVPPGSGALKLEDPRLAMLMAAPGRTDDAPEHQRP
                     FIYAEPAAGRVFLWESWLRHEVMPHSGKGERISISFNYR"
     gene            953..1873
                     /locus_tag="VN034_01105"
     CDS             953..1873
                     /locus_tag="VN034_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWV59224.1"
                     /translation="MADFELIADGLRFPEAPVVMDDGSVIVVEIEAKRITRCWPGGKK
                     EVIATPGGGPNGLAIGPDGKLYCCNNGGFNYVEANGYLAPHGIADDYSGGRIERIDIA
                     TGAVEVLYKSGDHGVTLRGPNDIMFDAHGGFWFTDHGKVDYAARCHDIVGIFYAKADG
                     SFIEEVIFPSYNPNGVGLSPDGTKLYVAETYTCRLTQFNIIAPGKVDDSTGPGGPGIP
                     LYRPAGYKFFDSLAMEANGNICVATIGECGISVVGPDGQLVEFVATDDIFTTNIAFGG
                     PDRQDAYITLSGTGRLVKTRWARPGLQLQY"
BASE COUNT          359 a          623 c          661 g          346 t
ORIGIN      
        1 gtcgagatcg gccgcggtcg caaccggcag ctcggcgatc gtgcccgcgg tcgccgggtt
       61 atagaccggc cgttcgtcgc gcccctcgcc gctccgccat gcgccgtcga tgaagagctg
      121 gaggtcggcg tcgtaggtcg cggtgtcggt catgggcgtt gccttctaca cgccctctca
      181 gtccgttcgt gtcgagcgaa gtcgagacac ccatcggtgt agcgcaaggc cgaggggcgt
      241 ctcgacttcg ctcgacgcga acgggaaggg ggctgggtca atagatgcgg tccatatagg
      301 accggctcag cgatagttaa agctgatgct gatccgttcg cccttacccg aatgcggcat
      361 cacctcgtgc cgcagccagc tttcccacag gaagacgcgg ccggcggcgg gctcggcgta
      421 gatgaaggga cgctgatgtt cgggcgcgtc gtcggtgcgc cccggcgcgg ccatcagcat
      481 cgcgagccgc ggatcctcga gcttgagcgc gcccgatccc ggcggaaccg cgacgtagaa
      541 agttcccgac acgatgctgt gcgggtggat atgcccgctg tgggtgccgc cgggtttcaa
      601 gatattgacc cacaggctgt cgagtttgag cggcttggcg aggtcgaaat ggcacgcctt
      661 ggcgaaagcc ttcacttcct tgtcgagcag gcgcttcaaa tcgtggaacg cgggatcgcg
      721 ctcgggcaaa tcgccgaggc tcgcatagct cgtatagccc ttatagccat gctcgcggct
      781 ccagcgccgc ccggcgccat cctcttcggc gaacagacgg ctgctctcct ccagctcttc
      841 gagcagctcg ggcgtgccga tatcggcctc gtagaaattg gtggcgaaga gcgtgcgaac
      901 gggcatgatg cgcgcaaagc ataggataag agcaagagca agggagacgg ggatggccga
      961 ctttgaattg atcgccgacg gattgcgctt tcccgaggcg ccggtcgtga tggacgatgg
     1021 cagcgtcatc gtcgtcgaga tcgaggcgaa gcgcatcacg cgctgctggc ccggcggaaa
     1081 gaaggaggtc atcgcgaccc ccggcggcgg gcccaacggc ctcgcgatcg gccccgacgg
     1141 caagctgtac tgctgcaaca acggcgggtt caactatgtc gaggcgaacg gctatctcgc
     1201 gccgcacggc atcgccgacg attattcggg cgggcggatc gagcgcatcg acatcgcgac
     1261 cggggcggtc gaggtgctgt ataaatcggg tgatcatggc gtgactctgc gcggccccaa
     1321 cgacatcatg ttcgacgcgc acggaggctt ctggtttacc gatcatggca aggtcgatta
     1381 tgccgcgcgc tgccacgaca tcgtcggcat cttttacgcc aaggccgacg gcagctttat
     1441 cgaggaagtg atcttcccga gctataaccc caacggcgtc ggcctctcgc ccgacgggac
     1501 caagctctac gtcgccgaaa cctacacctg ccgcctgacc cagttcaaca ttatcgcccc
     1561 cggcaaggtc gacgacagca cggggccggg cggcccgggc attccgctct atcgccccgc
     1621 gggctataaa ttcttcgaca gtctcgcgat ggaggcgaat ggcaatatct gcgtcgcgac
     1681 gatcggcgag tgcggcattt cggtcgtcgg ccccgacgga cagctcgtcg aatttgtcgc
     1741 gaccgacgat atcttcacca ccaacatcgc cttcggcggg cccgaccggc aggatgccta
     1801 tatcaccctg tcgggaacgg gtcggctcgt gaagacgcgc tgggccagac cggggctgca
     1861 actgcagtac tgaccccgcg ccccattggg gaggcgagga tgagaatgat cttggtggcg
     1921 gcgctgctga tcgcggcgcc cgcaggcgcg caggggctga acgccgaaca ggccgacgcg
     1981 atcgtcaag
//
LOCUS       DATMSU010000043         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_48212, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000043 DATMSU010000000
VERSION     DATMSU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48212"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VN034_01110"
     CDS             <1..436
                     /locus_tag="VN034_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV59225.1"
                     /translation="ELGDIVGLSASACHRRIRALEAAGIIIGYTARLDPESIGLGLDV
                     FVDISLTSQSEEALTAFESAVKSFDEILECQLLSGASDYRLRVAARSVADFDRLHRHC
                     LSRLPGVAAMHSAFALRTIKAFEGYPVPGPSGGGEAKTSLAG"
     gene            515..1894
                     /locus_tag="VN034_01115"
     CDS             515..1894
                     /locus_tag="VN034_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWV59226.1"
                     /translation="MGSIREAKIMVKENPIHKQQIDPILMSERFPFYDLDGQLAACGA
                     EVHTIIAGREDAVARAYWDAFNRLPSVDRKVEGELYESYIRGSARHTAVKYADAAGQE
                     VATIACQNTHMARRVKLPLASVMSCIAESHRLTIEYVVEACIGDAERLARLTSAINRL
                     ALLEFDIMQRYAAKLDRAVFSSERQTLAGDFDRSIASLVQDTDGVREQLARQAASADQ
                     AAKGMIAKTSEVAAASEQSAMAMREAASTAAGLIRAIEDARSEVEASASVATRASEQA
                     GEAVAMSETLSHHAESIESILGLIREIAGQTNLLALNATIEAARAGESGRGFAVVAQE
                     VKSLANETARATDDIAGKITAIQQATRGSVSANQSIQQTIVEVQTSAQRIRDAMDAQA
                     QTVTSITAAVDETALAADSMSSTIASIRNDSGMVANEISVLSEEFAKMGGRLQQLEQA
                     ASEFSRRVA"
     gene            1947..2654
                     /locus_tag="VN034_01120"
     CDS             1947..2654
                     /locus_tag="VN034_01120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59227.1"
                     /translation="MSTHILITGASRGIGAAIADALTMDATKVVALSSADGDLSDPRA
                     PDRLWQASLDKLDGRIDVLVNNAGVFEANPLGDPNADWLANWNRTMQVNLTASAQLCR
                     HAVLHWHERGMADEERGGRIVNIASRAAYRGDSPAHWHYAASKSGMVAMTKTIARAYA
                     KDGILAFAICPGFTMTGMADDYLASRGGDKLLADIPLGRVAMPDEVGEMARWCALDAP
                     TSMTGAVLDVNGASYVR"
     gene            2655..>3253
                     /locus_tag="VN034_01125"
     CDS             2655..>3253
                     /locus_tag="VN034_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWV59228.1"
                     /translation="MSWIISLPCTRAEAEALSGEIPELDAMAEAPVVVTREIDEDAGL
                     WQLDAYMDDKPGAALLKLLQSFVPSAKGQRPVVAELPEEDWVTLSQQGLEPVRAGRFF
                     VHTSSYADRVPPGTTPFLIDASQAFGTGGHDTTAGCLAMLDRLAGQGASPRNIADIGT
                     GTGLLAFAAMALWPRAKVIASDIDPASIFVTRDNAGINGV"
BASE COUNT          575 a         1076 c         1107 g          495 t
ORIGIN      
        1 cgagctgggc gacatcgtcg ggctttcggc ctcggcgtgc caccgccgca tccgcgcgct
       61 cgaggcggcg gggatcatca ttggctatac cgcaaggctc gatcccgaga gcatcggcct
      121 cggcctcgac gtcttcgtcg acatcagcct gacgtcgcag agcgaggagg cgctgaccgc
      181 gttcgagagt gcggtcaaaa gcttcgacga gatattggag tgtcagctgc tttcgggcgc
      241 ctccgactac cggctgcgcg tcgccgcgcg gagcgtcgcc gatttcgacc ggctgcaccg
      301 ccactgcctg tcgcgcctgc ccggcgtcgc ggcgatgcac agcgcctttg cactgcgcac
      361 gatcaaggca tttgagggct atccggtgcc cggcccttcc ggcggcggcg aggccaagac
      421 ctcgctcgcg ggttaacgac gcttttacca ctcttaccgc atatcccgtt caccagtttc
      481 gcaggatgca tgcgggcggc agatccgcac gtccatggga tcgatcaggg aagccaagat
      541 catggtgaag gaaaatccga ttcataaaca gcagatcgat ccgattttga tgtcggaacg
      601 ttttcccttt tacgatctcg acggccagct cgccgcgtgc ggggccgaag tccacacgat
      661 catcgccggc cgcgaggatg cggtcgcgcg cgcctattgg gacgcgttca accgcctgcc
      721 gagcgtcgac cgcaaggtcg agggcgaact ttacgagagc tatatccgcg gcagcgcgcg
      781 ccacacggcg gtcaaatatg ccgacgccgc gggccaggag gtcgcgacga tcgcgtgcca
      841 gaacacgcat atggcgcggc gcgtgaagct gccgctcgcc tcggtcatgt cgtgcatcgc
      901 cgagagccat cgcctgacga tcgaatatgt cgtcgaggcg tgcatcggcg acgctgagcg
      961 gctggcgcgg ctgacgagcg cgatcaaccg gctcgcgctg ctcgaattcg acatcatgca
     1021 gcgttacgcg gcgaagctcg accgcgcggt gttttcgagc gagcgccaga cgctcgcggg
     1081 cgatttcgac cgttcgatcg cctcgctggt acaggatacc gacggcgtgc gcgaacagct
     1141 cgcgcggcag gcggcctcgg ccgaccaggc ggcgaagggc atgatcgcca agacgagcga
     1201 agtcgccgcc gcgtccgaac aatcggcgat ggcgatgcgc gaggccgcgt cgaccgctgc
     1261 ggggctgatc cgcgcgatcg aggatgcgcg cagcgaagtc gaagcgtcgg cgagcgtcgc
     1321 gacgcgcgcc tccgaacagg cgggcgaagc cgtcgccatg tccgaaacgc tgtcgcacca
     1381 tgccgagtcg atcgaatcga tcctcggtct gatccgcgag atcgcggggc agaccaacct
     1441 tctcgcgctc aacgccacga tcgaggcggc gcgcgcgggc gaatcgggcc gcggctttgc
     1501 cgtcgtggca caggaggtga aaagcctcgc caacgaaacc gcgcgcgcga ccgacgatat
     1561 cgcgggcaag atcaccgcga tccagcaggc gacgcgcgga tcggtgagcg cgaaccagtc
     1621 gatccagcag acgatcgtcg aggtgcagac ctcggcgcag cgcatccgcg acgcgatgga
     1681 cgcgcaggcg cagacggtca cctcgatcac cgccgcggtc gacgagaccg cgctcgccgc
     1741 cgattcgatg tcgtcgacga tcgcgagcat ccgcaacgat tcgggcatgg tcgcgaacga
     1801 gatcagcgtg ctgagcgagg agttcgcgaa gatgggcggg cggctgcagc aactcgaaca
     1861 ggccgcgagc gaattcagcc gccgggtggc gtaaacacgc gctcctcttt tcttggcaag
     1921 ccccgccctg ctgcttaagg cggggcatga gcacgcatat cctgatcacc ggcgcgagcc
     1981 gcggcatcgg cgccgcgatc gccgacgccc tcaccatgga cgcgacaaag gtcgtcgcgc
     2041 tgtcgagcgc cgatggcgac ctgtccgatc ccagggcgcc cgaccggctg tggcaggcga
     2101 gcctcgacaa gctcgacggc cgcatcgatg tgctcgtcaa caatgccggc gtgttcgagg
     2161 cgaacccgct gggcgacccc aacgccgact ggctcgcgaa ctggaaccgc acgatgcagg
     2221 tcaatctgac cgcgagcgcg caattgtgcc gccatgcggt gctgcactgg cacgaacgcg
     2281 gcatggcaga cgaagaaagg ggcggccgca tcgtcaatat cgcgagccgc gccgcctatc
     2341 gcggcgacag ccccgcgcac tggcattatg cggcgtcgaa atcgggcatg gtcgcgatga
     2401 ccaagacgat cgcgcgcgcc tatgcgaagg acggcatcct cgcctttgcg atctgtccgg
     2461 gtttcacgat gacgggcatg gccgacgact atctcgcgag ccgtggcggc gacaaattgc
     2521 tcgccgacat tccgctcggg cgcgtcgcga tgcccgacga ggtgggcgag atggcgcgct
     2581 ggtgcgcgct cgacgcgccg acgtcgatga ccggcgcggt gctcgacgtg aacggcgcga
     2641 gctatgtccg ctaaatgagc tggatcatct ccctcccctg cacgcgcgcc gaggccgaag
     2701 cgctgtcggg cgaaattccc gaactcgacg cgatggccga ggcgcccgtc gtcgtgacgc
     2761 gcgaaatcga cgaggacgcg gggctgtggc agctcgacgc ctatatggac gacaagccgg
     2821 gcgcggcgct gttgaaactg ttgcagtcgt tcgttccgag cgcgaagggt cagcggccgg
     2881 tcgtcgcgga acttcccgaa gaggattggg tgaccctgtc gcagcagggg ctcgagcccg
     2941 tgcgggccgg acgctttttc gtccacacga gcagctacgc cgaccgcgtc ccgcccggca
     3001 cgacgccgtt cctgatcgac gcgagccaag cgttcggcac cggcgggcac gatacgacgg
     3061 cgggctgcct cgccatgctc gaccggctag cggggcaagg cgcgagcccg cgcaatatcg
     3121 ccgacatcgg caccgggacc gggctgctgg cctttgccgc gatggcgtta tggccgcgcg
     3181 cgaaggtgat cgcatcggac atcgaccccg cgagcatttt cgtgacgcgc gataacgccg
     3241 gcatcaacgg tgt
//
LOCUS       DATMSU010000044         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_29353, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000044 DATMSU010000000
VERSION     DATMSU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29353"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VN034_01130"
     CDS             <1..371
                     /locus_tag="VN034_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HWV59229.1"
                     /translation="PEAPHLERRIGFAIVARSPYPRLAAFAREQGWDHLKLYSDPTGD
                     FWQDYIGETRDADMAGYSVFTRKGGTIRHFWSVEGGPEIADPGQDPHGAPDPNPLWNL
                     LDLTPGGRGTDWYPSLRYDD"
     gene            396..815
                     /locus_tag="VN034_01135"
     CDS             396..815
                     /locus_tag="VN034_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59230.1"
                     /translation="MANPANSAPTGGLTPHIAIRDRRAAEAIDFYTKAFGAAEVARVP
                     ADDGVRLMHAHLHINGASLMMHDEFPEYVSEPSGKPEGVTLHLQVDDTDAWYDRAIAA
                     GAVSVMAPENMFWGDRYAQVRDPFGHLWSIGHPLKGE"
     gene            818..1312
                     /locus_tag="VN034_01140"
     CDS             818..1312
                     /locus_tag="VN034_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59231.1"
                     /translation="MTKTPMTLCLWYEGDAEEAAAFYAKTFADSEVGAVNRAPADSPG
                     GKEGDVQTVEFTLLGTPCVGLNGPPIFKHSEAFSFQIQTDTQEETDRLWNAIVDNGGQ
                     ESVCGWCKDKWGVSWQITPRVLLDATTGSDREAAKRAFEAMMTMKKIDIAKIEAAVRG
                     EAVA"
     gene            1335..1844
                     /locus_tag="VN034_01145"
     CDS             1335..1844
                     /locus_tag="VN034_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59232.1"
                     /translation="MMSEGSEPVTMCLWYDHDAEAAAKFYAATFPDSEFVAANRSPSD
                     FPDGKEGDVLTVEFTVMGVPFIAINGGPAFKQDEAFSIVINTDTQEETDRYWNAIVGN
                     GGAESMCGWCKDKWGVNWQITPRVLTQAMTHPDRAAAKRAMDAMMTMKKIDIAKIEAA
                     LKGETVGAA"
     gene            1861..2514
                     /locus_tag="VN034_01150"
     CDS             1861..2514
                     /locus_tag="VN034_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV59233.1"
                     /translation="MLIFYGHPFSSYTWKALIALYAKGVDFDYRSVDPAFPDHMPELR
                     AHWPVGQFPLLVHDGVPYFESSIIIEYLDHLVPEPRLIPQDFDAALKVRFFDRIFDNH
                     VMGRTQAVVADAIRGPENHVPLIVEQSKAALETVYGWLDKELAGGGWATPDGFTLADC
                     AAAPSLFYADWVHPIPERHENLRAYRARLLAHPSVSRCVEDARPYRPYFPLGAPDRA"
     gene            2702..3469
                     /locus_tag="VN034_01155"
     CDS             2702..3469
                     /locus_tag="VN034_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59234.1"
                     /translation="MGSPSAFLRTLIVAALAAVLAFAPAHAQQIVTITTTVTWTYEDG
                     DAEFIDEEAALLDDEMLAEDGDFAATRRYAPSANLATLGHAKARFGPFAVIDGTTARM
                     AGDVTSASPRQFAAMLAAFPGLKRLEMVDCPGSLDEEANLILARAIRRQGMETVVPAG
                     GSIRSGAVELWLAGVTRRAAPDAEFGVHSWADEYGREANDYPANDPVHAEYLGLYREM
                     GMDAAKAREFYALTNATPFDEVRYLTRGDMARFVALD"
     gene            3816..4124
                     /locus_tag="VN034_01160"
     CDS             3816..4124
                     /locus_tag="VN034_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HWV59235.1"
                     /translation="MAKRSLYQRMRSNAQVRTALFALGILMMIAGIAIGPLPGPGFLI
                     VFPLGLMLCLQNSAWAKRVYVRFKWRHPKYGAWADRIMRRVSAARRRARSALETPDGD
                     "
BASE COUNT          766 a         1348 c         1435 g          724 t
ORIGIN      
        1 atcccgaggc gccgcacctc gagcggcgga tcggcttcgc gatcgtcgcg cgctcgccct
       61 acccgcgcct cgccgccttc gcgcgcgagc agggctggga tcatctgaag ctctattcgg
      121 acccgaccgg cgatttctgg caggattata tcggcgagac gcgcgacgcc gacatggcgg
      181 ggtatagcgt cttcacgcgc aagggcggga cgatccgcca tttctggagc gtcgagggcg
      241 gccccgagat cgccgatccg ggacaggacc cgcacggcgc gcccgacccc aatccgctgt
      301 ggaacctgct cgacctgacc cccggcgggc gcgggaccga ctggtatccg agcctgcggt
      361 acgacgactg aaaccatagg aagaggagag agacgatggc gaaccccgcg aacagcgctc
      421 cgaccggcgg cctgaccccg catatcgcga tccgcgaccg gcgcgcggca gaggcgatcg
      481 acttttacac caaggccttc ggcgcggccg aggtcgcgcg cgttcccgcc gacgatgggg
      541 tgcggctgat gcacgcgcat ctccatatca acggcgcgtc gctgatgatg cacgacgaat
      601 ttcccgaata tgtcagcgag ccgtcgggca agcccgaagg cgtgacgctc catctgcagg
      661 tcgacgacac cgacgcctgg tacgaccgcg cgatcgccgc gggggcggtg tcggtgatgg
      721 cgcccgaaaa tatgttctgg ggggatcgct acgcgcaggt gcgcgacccg ttcggccatc
      781 tctggtcgat cggccatccg ttgaaaggag aatgaaaatg acgaagaccc cgatgacgct
      841 ctgcctctgg tacgaaggcg atgccgaaga agccgcggcc ttttacgcga aaacctttgc
      901 cgacagcgaa gtcggcgcgg tgaaccgcgc cccggcggat tcgccgggcg gcaaggaagg
      961 cgatgtccag acggtcgaat tcaccctgct cggcacgccg tgcgtcggtt tgaacggccc
     1021 gccgatcttc aagcatagcg aggccttttc gttccagatc cagaccgaca cgcaggaaga
     1081 gaccgaccgg ctgtggaacg cgatcgtcga taacggcggt caggaaagcg tgtgcggctg
     1141 gtgcaaggac aaatggggcg tctcgtggca gatcaccccg cgcgtcctgc tcgacgcgac
     1201 gaccggcagc gaccgcgaag cggcgaagcg cgcattcgag gcgatgatga cgatgaagaa
     1261 gatcgacatc gcgaagatcg aggccgcggt gcgcggcgaa gcggtcgcct gacggccgcg
     1321 gacaagggag aaggatgatg agcgaaggaa gcgaaccggt cacgatgtgc ctgtggtacg
     1381 atcatgacgc cgaggcggcg gcgaaattct atgccgcgac ctttccggac agcgaattcg
     1441 tcgcggcgaa ccgctcgccg agcgactttc ccgacggcaa ggaaggcgat gtgctgacgg
     1501 tcgaattcac cgtgatgggc gtgcccttca tcgcgatcaa cggcggcccc gcgttcaaac
     1561 aggacgaggc cttttcgatc gtgatcaaca ccgacacgca ggaagagacc gaccgttact
     1621 ggaacgcgat cgtcggcaat ggcggggcgg agagcatgtg cggctggtgc aaggacaaat
     1681 ggggggtcaa ctggcagatc accccgcgcg tgctgacgca ggcgatgacc catcccgacc
     1741 gcgcagcggc gaagcgcgcg atggacgcga tgatgacgat gaaaaagatc gacatcgcga
     1801 agatcgaggc ggcgctgaag ggcgaaaccg tcggcgcggc gtagccagga aaggcaatcg
     1861 atgctgattt tttatggaca ccccttctct tcctacacgt ggaaggcgct gatcgcgctt
     1921 tacgcgaagg gggtcgattt cgactatcgc tcggtcgatc cggcgttccc cgatcatatg
     1981 cccgaattgc gggcgcactg gccggtcggg cagtttccgc tgctcgtcca cgacggtgtg
     2041 ccttatttcg aatcgtcgat catcatcgaa tatctcgacc atctggtgcc cgagccgcgg
     2101 ctgatcccgc aggatttcga cgcggcgctg aaggttcgct tcttcgaccg catcttcgac
     2161 aatcatgtga tggggcggac gcaggcggtg gtcgccgacg caattcgcgg gcccgaaaat
     2221 catgtgccgc tgatcgtcga gcagtcgaag gcggcgctcg aaaccgtcta tggctggctc
     2281 gacaaggagc tggcgggcgg cggctgggcg acgcccgatg gcttcacgct cgccgattgc
     2341 gcggcggcgc cgtcgctttt ctatgccgac tgggtccacc cgattcccga gcggcacgag
     2401 aatctgcgcg cctatcgcgc gcggctgctc gcgcatccgt cggtttcgcg ctgcgtcgag
     2461 gatgcgcggc cgtatcgccc ctattttccg ctcggcgcgc ccgaccgggc ctgaacttat
     2521 ctttggtcat cccggcgaag gccgggatct caccgatgcg tcctaatgca aagttgagat
     2581 cccggccttc gcctggatga cgggaatatg ggttcgtgtc caacgtcccg aaacggggtt
     2641 aacgccgcgc tgacgctgcc tgtttgccag ttgatcatcg cgcccgcgcg aaggcggggg
     2701 catgggttcg ccgtccgcct tcctccgcac gctgatcgtc gccgctttgg cggcggtgct
     2761 ggcgttcgcg ccggcgcatg cgcagcagat cgtgacgatc actacgacgg tgacgtggac
     2821 ctatgaggac ggcgacgccg aatttatcga cgaagaggcg gcgctgctcg acgacgagat
     2881 gctggccgag gatggtgatt tcgcggcgac gcggcgttac gccccgtcgg cgaacctcgc
     2941 gacactcggc catgcgaagg cgcggttcgg gcccttcgcg gtgatcgacg gaacgaccgc
     3001 gcggatggcg ggcgacgtca cgtcggcgag cccgcgccag ttcgcggcga tgctcgcggc
     3061 ctttccgggg ctcaagcggc tcgagatggt cgattgcccc ggcagcctcg acgaggaagc
     3121 gaatctgatc ctcgcgcgcg cgatccgccg gcagggcatg gaaacggtcg tgcccgcggg
     3181 cggatcgatc cgatcgggcg cggtcgagct gtggctcgcc ggcgtcaccc gccgcgccgc
     3241 gcccgacgcc gaattcggcg tgcatagctg ggccgacgaa tatggccgcg aggcgaacga
     3301 ctatcccgcg aacgatccgg tgcacgccga atatcttggg ctttaccgcg aaatggggat
     3361 ggacgccgcg aaggcgcgcg aattttacgc gctgaccaat gcgacgccgt tcgatgaggt
     3421 gcgttatctg acgcgcggcg acatggcgcg ttttgtggcg ctggactgaa tagcgtcggc
     3481 gcggcgttcg aggaggctgc gcgaccggta ggggacattg agaaatatgc gtcagcccct
     3541 cctcttcaga ggagggtagc gagacttacg aacttgttcg ttagtcgcag cggggtggtg
     3601 cttgcggggg gagcgtgcgc gtgccgctaa caccaccccg ttcgactaaa ggcccggctg
     3661 cgccggacca agtctcactg cccctcctct gaagaggagg ggtttgggca acggtagctc
     3721 accatcccga aaccgccact cgcgatcatc gccccctcgc gcgccaagct gctttagcct
     3781 tgtaacacgc cgccgccttc cccacatcgc cccctatggc gaagcggagc ctctatcagc
     3841 ggatgcggtc aaatgcgcag gtgcgcaccg cgctgttcgc gctcggcata ttgatgatga
     3901 tcgcgggcat cgcgatcggg ccgctgcccg ggccgggatt cctgatcgtg tttccgctgg
     3961 ggctgatgct ctgcctgcag aacagcgcgt gggcgaagcg cgtctatgtc cgtttcaaat
     4021 ggcgccaccc caaatatggc gcatgggccg accggatcat gcgccgcgtc agcgccgcgc
     4081 ggcggcgcgc gcgctccgcg ctggaaacgc cagatggcga ttgactttcc accgactctt
     4141 gcttatccgc gcctccaaca gtggccgccc acgcttggcg gcccttttct gttgcagcat
     4201 ttgacgagat ctagggaaaa ccgcgatgaa gcgcacttat caaccgagcc gcctcgtgcg
     4261 caagcgccgt cac
//
LOCUS       DATMSU010000045         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_57729, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000045 DATMSU010000000
VERSION     DATMSU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57729"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1929)
                     /locus_tag="VN034_01165"
     CDS             complement(<1..1929)
                     /locus_tag="VN034_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59236.1"
                     /translation="MPASTERPRKGGGGNATASGILYQGGVGALFASRMLVELPLGNL
                     LGLADTRIKSIRFETNAPVDDILLETDQGGFVAIQAKSTITMGTALDSALGSVVDQFV
                     RFWIAAKGGSSLEVWARPLDPTRDRLLMVYGPGSGGTIARLANAVAGMRPDGPLGELR
                     DTFENLIRQSFAANGQEADGEEIAQLIRCIAFIELDPDDRDRQLAIEALTPVLVSRDE
                     ANRCFDVLSQISIEQMKRRGGFDSALLRSELTRHGVAPGASPTFARSINTLKDYSAAT
                     TVQLAAYEQLTPKEGDPFTLQRDCLPDILAAARTGSLILTGEPGSGKSGVLNSLSRAL
                     AADGAPIVALAVDRLPVATLNELSDQLGLEHSVLDVLANWPGSGPCYLVIDALDATRG
                     GPAEAVFKALIAGTLALAGHRWRVIVSIRSFDLRLGRQFRELFKGEPPSADLADPAFK
                     MVRHVSVPEWTDREFAVILGRSPDIGTAIEASGAALAALARVPFNTRLLADLLSRGTS
                     PSAFAGIGTQASLLAYFWQERVEVHGSGAALCLRATIDAMIATSGLHAAALEVARQVS
                     AIDLDQMEREGVLVRSNNGRDLAFRHHILFDFAASRLLLDPSNPKVLGERLVGPAALA
                     LAPALGFVLNEAWAADEDH"
BASE COUNT          518 a          878 c          856 g          466 t
ORIGIN      
        1 gtggtcctca tccgccgccc aggcctcgtt gagaacgaag ccgagcgcgg gcgccaaggc
       61 gagcgcggcc ggcccgacca gccgctcgcc cagcaccttg gggttggacg ggtcgagaag
      121 cagccggcta gcggcgaagt cgaacaggat atgatggcga aacgccagat cgcggccgtt
      181 gttcgagcgg accagcacgc cctcacgttc catctgatcg aggtcgattg cagacacttg
      241 gcgcgccacc tcaagggcgg cggcgtgcag gccactcgtg gcgatcattg cgtcgatcgt
      301 ggcgcgcagg cacaaggccg caccgctgcc atggacctcg actcgctcct gccagaaata
      361 ggcgagcagg ctcgcctgag tgccgatccc ggcaaacgcg ctcgggctgg tcccgcgcga
      421 cagaagatca gcgagcagcc gtgtgttgaa cggtacgcgg gccagcgccg cgagcgctgc
      481 gccgctcgcc tcgatcgccg tcccgatatc gggcgagcgc ccgaggatca ccgcgaattc
      541 gcggtcggtc cattcgggca ccgacacgtg ccgcaccatc ttgaaagccg ggtcggccag
      601 gtccgcactc ggcggctcgc ccttgaagag ctcgcgaaac tgtcgaccca ggcgcagatc
      661 gaagctgcgg atcgaaacga tcacgcgcca gcgatggccg gcgagggcga gcgtgcccgc
      721 gatcaatgct ttgaataccg cctcggccgg accgccgcgc gtcgcgtcca gcgcgtcgat
      781 caccagatag cacgggccgc tccccggcca gttggccagc acgtcgagca cggaatgctc
      841 gaggccgagc tgatcggaaa gttcgtttaa cgttgcgacc ggtagccgat ccaccgctaa
      901 cgcgacaatc ggcgcgccgt ccgcggccaa ggcgcgcgac agcgaattga gcacgccgct
      961 ctttcccgag ccgggttctc cggtcaggat cagcgaaccg gtcctcgccg ctgccagaat
     1021 gtcgggcaga cagtcgcgtt gcagcgtgaa gggatcgcct tcctttggcg tgagctgttc
     1081 gtaggcggcg agctgcacgg tggtcgccgc cgaataatcc ttgagcgtgt tgatcgaccg
     1141 cgcaaaggtg gggctcgccc ccggcgcaac gccgtgtcgc gtcaattccg atcgcagcag
     1201 cgcgctgtcg aaccctcccc gtcgtttcat ctgctcgatg ctgatctggg agagcacgtc
     1261 gaagcagcga ttggcctcgt cgcgcgaaac cagcaccggg gtcagcgcct cgattgccag
     1321 ttgccgatcg cgatcgtctg ggtcgagctc gatgaatgcg atacagcgga tgagctgggc
     1381 aatctcctcg ccatcggcct cttggccgtt cgccgcaaag gactggcgaa tcagattttc
     1441 gaacgtatcg cgcagctcgc ccaagggacc gtccggccgc atcccggcca ccgcattcgc
     1501 aaggcgcgcg atcgtaccac cagaccctgg accgtaaacc atcagtagcc ggtcgcgtgt
     1561 cggatcgagc gggcgcgccc agacctcgag gctgctgccg ccctttgctg cgatccagaa
     1621 gcgcacgaac tgatcgacga ctgagcccaa ggcgctatca agcgccgttc ccatcgtgat
     1681 cgtcgacttc gcttggatcg cgacgaaccc gccctggtcg gtttcgagaa ggatatcgtc
     1741 gaccggcgcg ttcgtttcga accggatcga tttgatccgt gtgtcagcga ggccgagcaa
     1801 attgccaagt ggcagttcga cgagcattcg cgatgcgaac agcgcgccga cgccaccttg
     1861 gtaaagaatg cccgaggccg tcgcgttgcc gccgccgccc ttgcggggtc gttcggttga
     1921 tgcgggcaag ctcgatctct cctttcagtc ggcggcagtt gacgctatct agcgcgccca
     1981 tcgtcgcaat gtcagcgaca taggtgctac acgcccacta ttttctaatc cagacatgga
     2041 gcgtcgaccg acagtccttc gctgactggt agaggttgag cagctcctcg acgagagtca
     2101 tcacttccga actatgcccg aatcgctgga actcgcggcg aatgaccgag gtgggcgcgt
     2161 aactgcccta acagacagcg gcctaacgaa cgtccgctta gtccatccga gccccgtaag
     2221 cggacgggcc gcttcccccc aattttgttg gtcgggcttt cttgaacgaa cggtagcttc
     2281 caggatcgat caactgggtc cgggatgacc gggattggcg cgctgccgcc ggaccgctcc
     2341 gcgggacggg aatggctggt tccggcagga gcagccattc acgcgcgcgg tctggaacgg
     2401 cttaagttgg tcggcagccg acaccgagtg agcgtcagct atctcagaaa cccaactcgg
     2461 aaccggacgg tcgcctaccg gccagtcggg gttacacaca atcggaaagc ggacggactg
     2521 cacctgagag ccgaaaaacg gcctctgaat gactgaaatg ggtcgatggt tgccggcagc
     2581 gtatgccgcg tcgacagtca ttgtagcgcg acccgaaaaa ggccgcttct aacacccaaa
     2641 catcttaaat cttctcaatc gtatgctgga gcggccgcaa tcgcaggaac gtagaaagct
     2701 gcgatcttgt cgatgaac
//
LOCUS       DATMSU010000046         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181018,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000046 DATMSU010000000
VERSION     DATMSU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181018"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1335
                     /locus_tag="VN034_01170"
     CDS             100..1335
                     /locus_tag="VN034_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWV59237.1"
                     /translation="MSETSTDDHGFNPRLIAAVVAIGVVAFIALWALIALGPQLSSGN
                     DGGGHALSKAAPGYAAIVDLAERAGAEVELRRRVETTRYEEYEPLLILTPTHRTRATE
                     IAELLDAQISAPALIILPKWQTMAVPGQTQKPGWVSVGLPVKPRDAMLPLAHFGKVAI
                     KAAPGQARAMPAHLAGHSFTAWLPANVQTISGGNLETLIAAPGGGAVLAQVGDRDIYI
                     LADPDLINNLAFASRDRAVGGAMLIDAIADYADADGLAFDVTLNGFGGQRSLLRFAFV
                     PPFIGITLCLLAAGLLALWQAWMRFGPALKPGRAIPVSKAALIANSADLIRQARRELD
                     GAEAYVKSQRIAIARRLHAPTGLDAAETDAWIDRHLGKGSDLFSALARRLPLARNTHE
                     FLADAQALHDIRKDLLRDS"
     gene            1325..>2099
                     /locus_tag="VN034_01175"
     CDS             1325..>2099
                     /locus_tag="VN034_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV59238.1"
                     /translation="MTANIESVQALGAAIEGEVAKAVFGQGPLTRMVTIALLAGGHVL
                     LEGPPGTAKTLLAQAFARATGLDFGRIQFTPDLMPGDILGSNLFNFQTSSFTLTKGPI
                     FTELLLADEINRTPPKTQAALLEAMQERRVTVGGESHVMSPRFTVLATQNPIEQQGVY
                     PLPEAQLDRFLFKLVVDYPDADEERRIVADHGGRFKSPAVGDFGVTQVADAKAIGEAI
                     DTIAAVRLAEEIVDYIVRLVRATRESADLECGASPRAATL"
BASE COUNT          343 a          727 c          703 g          326 t
ORIGIN      
        1 gatcagcctg ttcggcgggc ggtcgatcga cgccggggcg tgggagcaat gccgcagcgc
       61 ctatgccgaa ctgacggtgc cgaagaattg ggcgcgcgca tgagcgagac ctccaccgac
      121 gatcatggct tcaacccgcg actgatcgcc gcggtcgtcg cgatcggcgt cgtcgccttc
      181 atcgcgctct gggcgctgat cgcgctcgga ccgcagctca gcagcggcaa tgacggcggc
      241 ggacatgcgc tgtcgaaggc ggcgccgggc tatgccgcga tcgtcgacct tgccgagcgc
      301 gcgggcgccg aggtcgaact gcggcggagg gtcgagacga cgcgctacga agaatatgag
      361 ccgctgctga tcctcacgcc gacgcatcgc acccgcgcga ccgagatcgc cgagctgctc
      421 gacgcgcaga tcagcgcgcc agcgctgatc atactgccca aatggcaaac gatggcggtg
      481 cccggccaga cgcagaagcc cggctgggtc agcgtcggcc ttccggtcaa gccgcgcgat
      541 gccatgcttc cgctcgcgca tttcggcaag gttgcgatca aggccgcgcc gggccaggcg
      601 cgggcgatgc ctgcgcacct tgcgggacac agcttcaccg cctggctgcc ggccaatgtg
      661 cagacgattt cgggcggcaa tctcgaaacg ctgatcgccg cgccgggcgg cggggcggtg
      721 ctggcgcagg tcggcgaccg cgatatctat attcttgccg accccgacct catcaacaat
      781 ctcgccttcg cctcgcgcga cagggcggtc ggcggcgcga tgctgatcga cgccatcgcc
      841 gactatgccg atgccgacgg cctcgccttc gacgtgacgc tcaacggctt cggcgggcag
      901 cgttcgctgc tccgcttcgc gttcgtcccg ccctttatcg gcatcacgct ctgcctgctc
      961 gcggcggggc tgctcgcgct gtggcaggcg tggatgcgct tcggcccggc attgaaaccg
     1021 ggccgggcga tcccggtgtc gaaggcggcg ctgatcgcga acagcgccga cctgatccgg
     1081 caggcgcgcc gcgaactcga cggcgccgaa gcctatgtca aaagccagcg catcgcgatc
     1141 gcgcggcggc tgcacgcgcc caccgggctc gacgccgctg aaaccgacgc gtggatcgac
     1201 cggcacctgg gcaagggcag cgatctcttc tccgcgctcg cccggcgcct gccgctcgcg
     1261 cgcaacaccc atgaattcct cgcagacgcg caggcgctgc acgatatcag gaaggattta
     1321 ctccgtgaca gctaatatcg agagcgttca ggcgctcggt gccgcgatcg agggcgaggt
     1381 cgccaaggcc gtgttcgggc aagggccgct cacgcgcatg gtgacgatcg cgctgctcgc
     1441 gggcggccac gtcctgctcg aaggtccgcc gggaaccgca aagacgctgc tcgcgcaagc
     1501 gttcgcgcgt gcgacggggc tcgatttcgg ccgcatccag ttcacccccg acctgatgcc
     1561 gggcgacatt ttggggtcga acctgttcaa tttccagacg tcgagcttca ccctcaccaa
     1621 agggccgatc ttcaccgaat tgctgctcgc cgacgaaatc aaccgcaccc cgccgaagac
     1681 gcaggccgcg ctcctggaag cgatgcagga acggcgcgtg acggtgggcg gcgagtcgca
     1741 tgtcatgagc ccgcgcttca ccgtgcttgc gacgcagaac ccgatcgaac agcagggcgt
     1801 ctatccgctc cccgaagcgc agctcgaccg ctttctgttc aagctcgtcg tcgattatcc
     1861 cgatgccgac gaggaacgcc gcatcgtcgc cgaccatggt gggcgcttca aaagcccggc
     1921 ggtcggcgat ttcggggtga cgcaggtcgc ggatgcgaag gcgatcggcg aggcgatcga
     1981 caccatcgcg gcggtgcgcc tcgccgaaga gatcgtcgat tatatcgtcc gcctcgtccg
     2041 cgcgacgcgc gagagcgccg acctcgaatg cggcgcgtcc ccgcgcgccg cgacgttgc
//
LOCUS       DATMSU010000047         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_95784, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000047 DATMSU010000000
VERSION     DATMSU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95784"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..710
                     /locus_tag="VN034_01180"
     CDS             153..710
                     /locus_tag="VN034_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HWV59239.1"
                     /translation="MADTRLSDRRILIMATDGFEQSELEVPLERLEAAGADVDIAALG
                     TDDITGWDKDDWGEEVEVDLSIADAHVDNYDALVLPGGQINPDLLRVEPDAIALIKAF
                     AAAGKPVAAICHAPWLLVEAGLAKGRRLTSYKSIRTDVANAGAEMVDEAVVVDGNIIT
                     SRCPDDLPAFCDAIVEALVEAGAPV"
     gene            792..983
                     /locus_tag="VN034_01185"
     CDS             792..983
                     /locus_tag="VN034_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59240.1"
                     /translation="MSDHSSGDDGTQTGPGEQIRPKKGGKDPDAGSLGSGRAGGRPTE
                     DDLAKQADKDGARRSGGAK"
     gene            1232..1486
                     /locus_tag="VN034_01190"
     CDS             1232..1486
                     /locus_tag="VN034_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59241.1"
                     /translation="MAGDEEIWDQRGTLTLAHDESDGSEVPPLPEERPAPRPLREILG
                     RFAAAPLAERERYSLILENGQAFSFGDINAMLGREDSPFR"
     gene            1508..1885
                     /locus_tag="VN034_01195"
     CDS             1508..1885
                     /locus_tag="VN034_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59242.1"
                     /translation="MQDEVIAAAEAALRAAMLAGDVAALDALLDHDLSFTDQAGNRLS
                     KADDLAAYRSGRVKLETIDVVEPPDIRRWGDCATVCVTVDLAGFFGGESFFGRFAYSR
                     VWHESDGRWRVVLAHCSALPSTG"
     gene            1940..2119
                     /locus_tag="VN034_01200"
     CDS             1940..2119
                     /locus_tag="VN034_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HWV59243.1"
                     /translation="MADDKNQVGAADRSRISLGEDYEVRDWTASLGVSEQELREAVDA
                     VGNSADAVRGYLKRS"
     gene            2122..2391
                     /locus_tag="VN034_01205"
     CDS             2122..2391
                     /locus_tag="VN034_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59244.1"
                     /translation="MASEFDRAITSPLERVARVLAGHALSRNAEGDMPSAGEAVDALW
                     GEYSDAALAVLRTLREPSAAMLAVGDAGVWDRMIHAAIEEETISD"
     gene            2421..2684
                     /locus_tag="VN034_01210"
     CDS             2421..2684
                     /locus_tag="VN034_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HWV59245.1"
                     /translation="MANGWARDGAVQEQIDDTVADAVKLARLRLAEGTGTEFCEDCSE
                     RIPDARRNALPASRTCVACQSVRDGQRRQAGINRRGSKDSQLR"
     gene            2728..2985
                     /locus_tag="VN034_01215"
     CDS             2728..2985
                     /locus_tag="VN034_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59246.1"
                     /translation="MAADYERGRRDGLRLALAILSAEEEKWAPLLGESRSWRTNRDRE
                     VRHKALQVAQTRLQTALSKLAPLDREASDAELAAALAKVGL"
     gene            3118..3573
                     /locus_tag="VN034_01220"
     CDS             3118..3573
                     /locus_tag="VN034_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HWV59247.1"
                     /translation="MFDNKNIATLNSLIATTLDSVDGYRKAADEANAAQFREIFLSRA
                     TERQAVVTDFQAKVRELGGNPEDDGTVLASAHRAFLGLRDKLTGARDDEAVIAEVERG
                     EDHIKSKFESALGDADLDPAVRELIETGYASVRAGHDQMSALKHGLDAS"
BASE COUNT          711 a         1211 c         1329 g          627 t
ORIGIN      
        1 cgcacgccgc cctccgctgc gggctcgcca agagcgcgtc ttctggccct tcgggccgcg
       61 ctccgcttcg cggcgcggag ggggcgggaa agggatgccg ggcaacattg cggccgctcg
      121 agcattgtct ggatagcaaa ggagcttcca gcatggccga caccagatta tccgaccgcc
      181 gtatcctcat catggcgacc gacgggttcg aacagtccga actcgaggtg ccgcttgaac
      241 gtctcgaggc agccggcgcc gacgttgaca tcgccgcgct cggaacggac gacatcaccg
      301 gctgggacaa ggacgactgg ggcgaggagg tcgaggtcga cctcagcatc gccgatgccc
      361 atgtcgacaa ttatgatgcg ctcgttctgc cgggcggcca gatcaatcct gatcttctcc
      421 gcgtcgagcc cgacgcgatc gcgctcatca aggcgttcgc cgcggcgggc aaaccggtgg
      481 cggccatttg ccatgcgcca tggttgctcg tcgaagcggg gctagcgaag ggccgccggc
      541 tcaccagcta caagtcgatc cgcaccgacg tcgccaacgc cggcgctgag atggtggacg
      601 aggcggtcgt cgtcgacggc aatatcatca ccagccgctg ccctgacgac ctgcccgcct
      661 tttgcgacgc gatcgtcgag gccctggtcg aggcaggcgc gccggtttga cacgacccat
      721 ggcgcgcggc gcgcgcaacc acgcgccata gcgggtcgtc tcctttcata gcgaagacat
      781 ggagatgcac gatgagtgac cacagcagcg gcgatgacgg gacgcagacg ggccctggag
      841 aacagatacg tccgaagaag ggcggcaagg atcccgacgc gggctcgctt ggcagcggac
      901 gcgccggcgg acgtccaacc gaggacgatc tcgcaaaaca ggccgataaa gacggcgcac
      961 gccgcagcgg aggcgcaaaa tgagcgagtt tcgaaaggtt cgcgaggttc cgaccaaagt
     1021 cacgaagaac cccaacatct cgaccgagaa ccagccggag ggcgcggcgc gccggcccag
     1081 cgatacgctg gaccgcgagt ccgcgcggaa tcctgaaagc ggcggcagta tccgcgggaa
     1141 ttacaatccc gcgagcggca cgcggatcgg cgaggagacg gacaggcgcg cgggccgcga
     1201 cggcgaagga caaccgccgc aggacgctga catggccggc gatgaagaga tatgggatca
     1261 gcgcggaacc ttgacgctcg cgcatgacga gagcgatggt tccgaagtcc ctccgcttcc
     1321 ggaggaacgg ccggcgccgc gcccgctgcg cgagattctc ggacggtttg cggcggcgcc
     1381 gctggccgag cgcgagcgtt acagcctgat cctcgaaaac gggcaggcat tttcattcgg
     1441 tgatatcaac gccatgctcg gccgagaaga ttccccgttt cgctgagcga gcatgtccgg
     1501 agctgccatg caggatgaag tgattgccgc ggcggaggcc gcattgcgcg cggcgatgct
     1561 ggccggcgat gtcgcggcgc tcgacgcgtt gctcgaccac gacctctcct ttaccgatca
     1621 ggcgggtaat cgcctgtcca aggccgatga tcttgcggct tatcgatcgg gacgggtcaa
     1681 gctcgagacg atcgacgttg tcgagccccc ggacattcgc cgctggggcg attgcgcgac
     1741 cgtttgcgtg acggtcgatc ttgcgggttt cttcggcggc gagagcttct tcggccgctt
     1801 tgcctattcg cgggtgtggc acgagagcga tggccgctgg cgcgtcgttc tcgcgcattg
     1861 ctccgcgctt ccgtcaacgg gctgacccgc ctgcaacctc gagtgcctcg ggggcgtttg
     1921 tcggcaaagg agtgacgtca tggcagacga caaaaatcaa gtgggcgccg ccgaccgcag
     1981 ccggatcagc ctcggcgaag attatgaagt ccgcgactgg accgcctcgc tcggcgtgag
     2041 cgagcaggag ttgcgcgaag cggtcgatgc cgtgggcaat tccgccgacg ccgtgcgcgg
     2101 ctatctgaaa aggtcgtgac gatggcgagc gagttcgatc gcgcgatcac gtcgccgctc
     2161 gagcgcgtcg cgcgtgtcct cgcggggcat gcgctcagcc ggaatgccga gggcgatatg
     2221 ccgtcggctg gcgaggcggt cgatgcgctc tggggcgagt atagcgatgc ggcgctcgcc
     2281 gtgttgcgga cgctccgcga gccgagcgcg gcgatgcttg ccgtcgggga tgccggcgtc
     2341 tgggaccgga tgatccatgc cgcgatcgag gaagagacga tttccgactg acgggcgact
     2401 acgtgaccga ggtaaatgag atggcgaatg gttgggcgcg cgacggtgcc gtacaggagc
     2461 agatcgacga cacggttgcc gacgccgtaa agcttgcgcg gctccgcctg gccgaaggca
     2521 ccggcactga attttgcgaa gactgcagcg agcgcattcc ggacgcccgg cgcaacgccc
     2581 tgccggccag ccggacgtgc gtcgcctgcc agtcggtccg cgacggtcag cggcggcaag
     2641 ccgggatcaa ccgccgcggc agcaaggaca gccagcttcg ctaatgggat ggggacccgc
     2701 tgcctgagtt tttcaatgga ggtgaggatg gcggccgact atgagcgcgg gcggcgcgac
     2761 gggcttcgcc tcgcgcttgc catcctctcc gccgaggaag aaaaatgggc gccgctgctt
     2821 ggcgagagcc ggtcctggcg gacgaacagg gatcgggagg tccgccacaa ggcgctccag
     2881 gttgcgcaga cgcggctcca gaccgcgctg agcaagctcg ctcccctcga tcgcgaggca
     2941 tccgatgccg agctcgcggc cgcgctcgcc aaggtcgggc tgtaatggcg aactcctgca
     3001 cgcttcgtcg caaagctgcg tttgccccga tcgaccgggg caactagcgg gtcgcttgct
     3061 cgttctttcg tgaggcgccg gtcatgggat cggcccgctc ccccgaaagg aatgctcatg
     3121 ttcgataaca agaatatcgc aacgctgaac tccctcatcg cgaccacgct cgacagcgtc
     3181 gatggctatc gcaaggcagc cgacgaggcc aatgccgcgc agttcaggga gattttcctg
     3241 agccgtgcaa ccgagcggca ggccgtcgtc accgacttcc aggcgaaggt gcgcgaactc
     3301 ggcggcaacc cagaagatga cggaacggtg cttgcgtcgg cgcatcgcgc ctttctcggc
     3361 cttcgcgaca agctcaccgg cgcgcgcgac gatgaggcgg tgatcgccga ggtcgagcgc
     3421 ggcgaagatc atatcaagtc gaagttcgaa agcgcgcttg gcgacgccga tctcgacccc
     3481 gcggttcgcg agctcatcga gaccggctat gcctcggtcc gcgcgggcca cgaccagatg
     3541 tcggcgctga agcatgggct cgacgccagc tgatctttcc cggtagagaa ttccctcggc
     3601 caccccgggg cgaccgggac tcgattaccc gtcaagtacg cctttcggcg gcaatcgatc
     3661 ggttgcggtc tttgccgatc atcatatgaa gaacgtccgc ttctcgcgat ttatcgctag
     3721 aagcggacag tcttcaactg atgacgtgga cggctctcca acctcccgcg gtatgatccg
     3781 cgggatgagc tggaaggaga gctacgatgg agaagattgc aacagtcggg ttggatatcg
     3841 cgaagtcggt gtttcaggtc cacggcgttg atgctgca
//
LOCUS       DATMSU010000048         6492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_161905,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000048 DATMSU010000000
VERSION     DATMSU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161905"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1137)
                     /locus_tag="VN034_01225"
     CDS             complement(97..1137)
                     /locus_tag="VN034_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWV59248.1"
                     /translation="MSFPTSPEAMTTAWLGDVLGQPGRLRAFTAARVGTGQMCDSYRL
                     ALDWEDGVAAPASVIAKCPSHDEASRNIAKLTGTYVKEVSWYRELAADSGVAAPRCYH
                     AEIADDDVDFVLILSDLAPARQGDQLAGTGLAGLLPCIEAAAGLHALLWNDPRLEALP
                     WLSRDNGDLIRHLFPQFYLGFRERYAARLDPAVLDLGAGIVERLDAYLARVPAARTIV
                     HGDLRIDNILFAPGGDRCWLVDWQTLGRGSGAIDLAYLVGTSIADPVERAAADRPAFD
                     HWIGALGQLGVAPDEAGLWTDYRMGALSGYFMAVFASMSVERTTRGDEMFAVMAERPA
                     RQALALGSLELL"
     gene            complement(1134..2225)
                     /locus_tag="VN034_01230"
     CDS             complement(1134..2225)
                     /locus_tag="VN034_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59249.1"
                     /translation="MLTKGDDYPLHQTSEPVAFAGTDRNFYDRYFFNGYSPDGSVFFA
                     AAMGFYPQLGIVDASFCVIAGGVQHNLRASRRSGGDRLELSVGPIALEIDMPLEIVTL
                     RIAPNDGPLAGELRFTGRHFPIEEPRFIRRNGTRLFMDYTRMTQNGHWSGWLSVGGQR
                     IETGSGWAGTRDRSWGIRPIGASEPQPPPEGNFSQFFWLWTPCNFESSSLFFHSNDDD
                     AGKPWNRRAMVMGDGGTEQHFDAATFDIEWQAGSRRIAQMTADLGSGRSLTLTPTGPV
                     FAMSGLGYTHPTWGHGFDHGPGLAVAYDSLSETERGWGNPLAMHIQALVTATLTDGGS
                     VRRGAGVLEQLFVGPHEPTGLAGLMDPAP"
     gene            complement(2275..3123)
                     /locus_tag="VN034_01235"
     CDS             complement(2275..3123)
                     /locus_tag="VN034_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWV59250.1"
                     /translation="MTRLFHISDLHFGLEDRAALAWFTDCVRREAPDAVLITGDLTMR
                     ARSREFAAACDWIGALDVPVTVEVGNHDLPYFNPFERFFYPYRRIRGIERLVERELDL
                     PGVAVVPLKTTARAQWRLDWSKGWVTGRALEKTLAAIDTLPRGTTVLVTAHHPLVEAG
                     TKGRALTRGGERALAALAKRGVAAVLTGHVHDAFDLKKTTAAGPIRMIGAGTLSQRIR
                     STPPSFNDLTVEGAAIDVRVRNLDHVPTPDMQIDDVPPDALPPREPGEPVAPVAAVPP
                     VDPPVH"
     gene            complement(3120..3953)
                     /locus_tag="VN034_01240"
     CDS             complement(3120..3953)
                     /locus_tag="VN034_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWV59251.1"
                     /translation="MTTGNFTRPALVCNSQSGSHDDAVLAEIVESCRTAGAPLVAVFA
                     LPDDEIPDAGELVRQDIDLLLVWTGDGTINAAATGAAGWDGAILPLPGGTLNLLSKEL
                     HGDRPVTEILSDALQGKGRRQPVPMIRSDGGNAFITIVAGPATQWAEVRETMRQDGLI
                     EASRSAPDALDAMMNAPGVAVAGHGRAYPAIILTPTAHGIRADGILTEGTADVLRHGL
                     AWLGGDFRDGPSEEIISGETIILESGAPISLEYDGELGETPSPARFGLAASAVDFVAT
                     A"
     gene            4074..5000
                     /locus_tag="VN034_01245"
     CDS             4074..5000
                     /locus_tag="VN034_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59252.1"
                     /translation="MIKILFVVLLLVLLFGGGAKMIVASGAKSLNMADRLLGQGDGAR
                     LLLADQPYGRGPRQSLDIWVPENAKADDQLPVVVFFYGGGWDSGERGSYGFAGRALAR
                     QGFVVVIPDYRLVPKAHWPDFIEDSAAAVAWTHEHIAKLGGDPERIALMGHSAGAYNA
                     AMLALDPQWLRAVKSDPSIIRGVAGLAGPYDFLPLEKGGRGDRAMGKVKPIEKTQPIH
                     FVRGDAPPLWLATGDGDDTVRPRNSQNLAAAIEKAGGSATLRLYPGMGHTGIVMALAA
                     PFQNKGPVLDEATDFLRGVTGRRIVPTAEAAP"
     gene            4997..5791
                     /locus_tag="VN034_01250"
     CDS             4997..5791
                     /locus_tag="VN034_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWV59253.1"
                     /translation="MSAPIPAIVLAGSRPGPDPLLSGSGVSTKALMPIAGQAMLVHVV
                     KALRASPEVGPITILAQNSAELAAVPELAGLADLHFADSGQGISDSLAAALPPGDDPL
                     LVTTADNVLLTPAMVAEFLRGAEDSDVAVAMVERDVLLARYPQSKRTWLKFRGGWWSG
                     ANMFRLRGRRVLPLLDFWGRIERDRKKGLKIIAAFGPWLLMGALLRLFSIEQGVAHAG
                     LRFGLKATVVPMSEPEACIDADKPADIDLIEAIFAARRQDAIGQPL"
     gene            5788..>6492
                     /locus_tag="VN034_01255"
     CDS             5788..>6492
                     /locus_tag="VN034_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HWV59254.1"
                     /translation="MTIDKAIILSAGQGSRLLPLTRDIPKCLIEFNGRSLISWQVAAL
                     VANGIKDIVVVTGFRTERVEDHALQLYRDTGARIRTLFNPFFQVADNLGTCWIAREEM
                     DRDFIILNGDTIISDEIVAKLIAGANEPINVTVDVKADYDDDDMKVNRDAEGRLHHIG
                     KRLLPPDTNAESIGMLAFVGDGPSIFRNQVDQMMRTPDGVERWYLRAIDIIAKGNRVG
                     TVSIEGLEWQEVDFPQD"
BASE COUNT         1050 a         2172 c         2187 g         1083 t
ORIGIN      
        1 cgcgaaggcg ggggccgcca tcggccttac gctagctctc cagcggcccc cgccttcgcg
       61 ggggcgacga ctagtttcgg tcgtcagttg ctccggctac agcagctcca gactccccaa
      121 cgccagcgcc tgccgtgccg gccgctcggc catcacggcg aacatctcat cgccgcgcgt
      181 cgtgcgctca acgctcatcg acgcgaacac tgccatgaaa taaccgctga gcgcgcccat
      241 ccggtagtcg gtccacagcc cggcttcgtc gggcgcaacg ccaagctgtc cgagcgcgcc
      301 gatccaatgg tcgaatgccg gccggtcggc tgccgcgcgc tcgacgggat cagcgatgct
      361 ggtgccgacc agataggcga ggtcgatcgc cccgctgccg cgccccagcg tttgccagtc
      421 gacgagccag cagcggtcgc cgcccggggc aaagaggatg ttgtcgatgc gcaggtcgcc
      481 atgaacgatc gtccgcgccg cgggcacacg cgccagatag gcgtcgagcc gctcgacgat
      541 ccccgcgccg aggtcgagca ccgccggatc gagccgcgcc gcatagcgtt cgcgaaagcc
      601 gaggtagaat tgcggaaaga ggtggcggat cagatcgcca ttgtcgcgcg atagccaggg
      661 caaggcttcg agccgcggat cgttccagag cagcgcgtgc agacccgccg cggcctcgat
      721 gcaggggagg agcccggcaa gcccggtgcc ggcgagctgg tcgccctgcc gcgccggtgc
      781 gaggtcggaa aggatcaaca cgaaatcgac gtcatcgtcc gcgatctcgg catgatagca
      841 gcggggcgca gcgacgccgc tgtccgccgc gagttcgcga taccagctga cttccttgac
      901 ataggtgccg gtcagcttgg cgatgttgcg gctggcctcg tcgtggctcg ggcatttggc
      961 gatgacgctt gcgggcgccg cgacgccatc ctcccaatcg agcgcgaggc ggtagctgtc
     1021 gcacatctgg ccggtgccga ccctggcagc cgtgaaggcc ctcagccgtc ccggctgccc
     1081 cagcacatcg ccgagccatg ccgtcgtcat cgcctcgggc gaggtcggaa agctcaaggc
     1141 gcggggtcca tgaggccggc aaggccggtc ggctcgtgcg ggccgacgaa caattgctcg
     1201 agcacccccg cgccgcggcg gacgctgccg ccgtcggtca gcgtggcggt gacgagcgcc
     1261 tggatgtgca tcgccagcgg attgccccag ccgcgttcgg tttcggacag gctgtcatag
     1321 gcgacggcga gccccggccc gtggtcgaag ccatggcccc atgtcggatg cgtatagcca
     1381 agcccgctca tcgcgaagac cggccccgtg ggcgtcagcg tcaatgaccg gccgctgccg
     1441 aggtcagcgg tcatctgcgc gatccgccgt gatcccgctt gccattcgat gtcgaacgtc
     1501 gcggcgtcga aatgctgctc ggtcccgccg tcgcccatga ccatggcgcg gcggttccac
     1561 ggctttcccg cgtcgtcgtc gttgctgtgg aagaagagcg agctgctttc gaaattgcac
     1621 ggcgtccaca gccagaaaaa ttggctgaaa ttgccttcgg gcggcggctg cggttcgctc
     1681 gcgccgatgg gacggatgcc ccagctgcga tcgcgtgtcc ccgcccatcc gctgccggtc
     1741 tcgatgcgct gtccgccgac cgacagccag cccgaccaat ggccgttttg cgtcatgcgc
     1801 gtataatcca tgaacagccg cgtgccgttg cgacggatga agcgcggttc ctcgatcggg
     1861 aagtggcggc cggtgaagcg tagctcgccc gcgagcggcc cgtcattggg cgcgatccgc
     1921 agcgtgacga tttcgagggg catgtcgatt tcgagcgcaa tcggccccac cgacagttcg
     1981 agccggtcgc cgcccgatcg ccggctggcg cgaagattgt gctgaacccc gccggcgatg
     2041 acgcagaaac tcgcatcgac gatgccgagc tgcggataaa agcccatcgc cgcggcgaag
     2101 aagaccgagc cgtcgggcga atagccgttg aagaaatagc ggtcgtagaa gttgcggtcg
     2161 gtccccgcga acgcaaccgg ctcgctggtc tgatgcaaag gataatcgtc gccttttgtc
     2221 agcatatgtc ctcatcccgt tttttcttgc aacctaacgg gatggagcgt cgcgtcaatg
     2281 cactggcgga tcgacaggcg gcacagccgc gactggggca accggctcgc ctggttcgcg
     2341 cggcggcagc gcgtcgggcg gcacgtcgtc gatctgcata tcgggggtcg ggacatgatc
     2401 gagattgcgc acgcggacgt cgatcgcggc gccttcgacg gttaggtcgt tgaaactcgg
     2461 cggggtcgag cggatccgct gcgacagcgt gcccgccccg atcatgcgga tcgggcccgc
     2521 ggcggtcgtc tttttcaggt cgaaagcgtc gtggacatgg ccggtcagca ccgccgccac
     2581 gccgcgcttc gccaaggccg cgagcgcgcg ttcgccgccg cgcgtcaatg cacgtccctt
     2641 ggtcccggcc tcgaccagcg ggtgatgcgc ggtgaccagc accgtcgttc cgcgaggcaa
     2701 ggtgtcgatc gccgcgagcg tcttttcgag cgccctgccc gtcacccatc ccttcgacca
     2761 gtcgagccgc cattgcgcgc gtgccgtcgt cttcagcggc accacggcga cgccgggaag
     2821 atcgagttcg cgttcgacca gccgctcgat cccgcggata cggcgatagg gataaaagaa
     2881 gcgttcgaag gggttgaaat agggtaggtc gtgattgccg acttcgacgg tcaccggcac
     2941 gtcgagtgcg ccgatccagt cgcacgcggc ggcgaattcg cggctccgcg cgcgcatcgt
     3001 gagatcgccc gtgatcagca ccgcatcagg agcctcgcgc cgcacgcaat cggtgaacca
     3061 ggcgagcgcg gcgcggtctt cgagcccgaa atgcaggtcg ctgatgtgga agagccgggt
     3121 catgccgtcg cgacgaagtc cacggcgctc gcggcgagcc cgaagcgggc gggtgagggc
     3181 gtttcgccga gttcgccgtc atattcgagg ctgatcggcg cgccgctttc gaggatgatc
     3241 gtttcgccgc tgatgatttc ctcgctcggc ccgtcgcgaa aatcgccgcc gagccacgca
     3301 agaccatggc gcagcacgtc ggcggtgccc tcggtcaata tgccgtcggc ccggatgccg
     3361 tgcgccgtcg gggtaaggat gatcgcggga taggcccgac catgtccggc aaccgcgacc
     3421 ccgggggcgt tcatcatcgc gtcgagcgca tcgggtgccg accggctggc ttcgatcagg
     3481 ccgtcctgcc gcatcgtttc gcgcacttcg gcccattgtg tcgcggggcc ggcgacgatc
     3541 gtgatgaagg cgttgccgcc gtcggatcgg atcatgggga ccggttgccg ccggcccttg
     3601 ccctgcaacg catccgacag gatttcggtc acgggccggt cgccgtgcag ttccttcgac
     3661 aaaaggttga gcgtcccgcc gggcagcggc aggatggcgc cgtcccagcc cgccgccccg
     3721 gtcgccgcgg cgttgatcgt cccgtcgccc gtccatacga gcagcaggtc gatgtcttgc
     3781 cggacgagtt cgccggcatc gggaatctcg tcatcgggca gcgcgaagac ggcgaccagc
     3841 ggcgcgcccg cggtgcggca gctttcgacg atctcggcaa ggacggcgtc atcatgactg
     3901 ccgctctggc tgttgcagac aagggcgggg cgggtgaagt ttcctgtcgt catcgcactt
     3961 tcctacgcgt gagggcggaa aaggtcgcgt ccctatcgcg cggccggctt gttcgccgct
     4021 ccgctgccct gtagggcgcg aaagatggcg cgccggtttc caaaggacag cgattgatca
     4081 agattctctt cgtggtcctg ctgctcgtgc tgctgttcgg cggcggcgcc aagatgatcg
     4141 tcgcgagcgg cgcgaagtcg ctcaacatgg cggaccggct gctcgggcag ggtgatggcg
     4201 cgcggctgtt gctcgccgac cagccctatg gccgcggccc gcgccagtcg ctcgacatct
     4261 gggtgcccga aaatgccaag gcggatgatc agctgcccgt tgtcgtcttt ttctatggcg
     4321 gcggctggga cagcggcgag cgcggcagct atggcttcgc cgggcgggca ttggcgcggc
     4381 agggctttgt cgtggtgatt cccgactatc gcctcgtgcc caaggcgcat tggcccgact
     4441 ttatcgagga tagcgccgcg gcggtcgcgt ggacgcacga acatatcgcc aagcttggcg
     4501 gcgatcccga acggatcgcg ctgatgggcc attcggcggg cgcctataac gccgcgatgc
     4561 tcgcgctcga cccgcaatgg ctgcgtgcgg tgaagagcga tccgtcgatc atccgcggcg
     4621 tcgcggggct tgccgggccc tatgacttcc tcccgcttga aaagggcgga cgcggtgaca
     4681 gggcgatggg caaggtcaaa ccgatcgaga aaacgcagcc gatccatttc gtgcgcggcg
     4741 atgcgccccc cttgtggctt gcgacgggcg acggggacga cacggtgcgt ccgcgcaaca
     4801 gccagaacct cgccgccgcg atcgaaaagg ccgggggctc ggcgacgctg cgcctctatc
     4861 cgggcatggg gcatacgggc atcgtcatgg cgctcgccgc gccgtttcaa aacaagggac
     4921 cagtgctcga cgaggccacc gatttcctgc gcggggtgac cggccgccgc atcgttccca
     4981 ccgcggaggc cgcgccgtga gtgccccgat ccccgcgatc gtcctcgcgg gcagccgtcc
     5041 cggccccgac ccgctgctat cgggaagtgg cgtgtcgacc aaggcgttga tgccgatcgc
     5101 gggacaggcg atgctcgtcc atgtcgtaaa ggcgctgcgc gcttcgcccg aagtcggccc
     5161 gatcacgatc cttgcgcaga acagcgccga gctggcggcc gtgccggaac tggccgggct
     5221 cgccgacctg catttcgccg attcggggca ggggatcagc gactcactcg ccgccgcact
     5281 gccgccgggc gacgatccgc tgctcgtcac gaccgccgac aatgttctgc tgacccccgc
     5341 gatggtcgcc gaattcctgc gcggcgccga ggacagcgat gtcgcggtcg cgatggtcga
     5401 gcgcgacgtg ttgctcgcac gctatccgca gtcgaagcgc acctggctga aattccgcgg
     5461 cggctggtgg tcgggcgcga acatgttccg gctgcgcggg cggcgcgtgc tgcccttgct
     5521 cgacttctgg ggccgcatcg aacgcgaccg caagaaggga ctcaagatca ttgccgcttt
     5581 cggcccctgg ctgctgatgg gcgcgctgct tcgcctcttc tcgatcgagc agggcgtcgc
     5641 gcacgccggg ctacgtttcg gtctcaaggc gacggtcgtg ccgatgtccg agcccgaggc
     5701 gtgcatcgac gccgacaagc ccgccgacat tgacctcatc gaagcgatat ttgccgcgcg
     5761 gcggcaagac gctatagggc agcccctatg accatcgaca aagccatcat cctgtcggcc
     5821 gggcaaggct cgcgcctgct gccgctgacc cgcgacattc ccaaatgcct gatcgaattc
     5881 aacggccgca gcctcatcag ctggcaggtc gcggcactcg ttgccaacgg catcaaggac
     5941 attgtcgtcg tcacgggctt tcgcaccgaa cgcgtcgagg atcatgcgct ccagctttat
     6001 cgcgacaccg gcgcgcgcat ccgcaccctg ttcaatccct tcttccaggt cgccgacaat
     6061 ctcggcacct gctggattgc gcgcgaggag atggaccgcg atttcatcat cctcaacggc
     6121 gacaccatca tttcggacga aatcgtcgcg aagctgatcg cgggcgcgaa cgagccgatc
     6181 aacgtcaccg ttgacgtcaa ggccgactat gacgacgacg atatgaaggt gaaccgcgac
     6241 gccgaaggcc gcctgcatca tatcggcaag cggctgctgc cccccgacac caatgccgaa
     6301 tcgatcggga tgctggcgtt cgtcggcgac ggcccgtcga tcttccgcaa tcaggtcgac
     6361 cagatgatgc gcacccccga cggggtcgag cgctggtatc tgcgcgcgat cgacatcatc
     6421 gccaagggca accgcgtcgg caccgtgtcg atcgaagggc tcgaatggca ggaagtcgac
     6481 ttcccgcagg ac
//
LOCUS       DATMSU010000049         9478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_38977, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000049 DATMSU010000000
VERSION     DATMSU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9478
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38977"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..1734)
                     /locus_tag="VN034_01260"
     CDS             complement(106..1734)
                     /locus_tag="VN034_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWV59255.1"
                     /translation="MRPSSWADRLLAFSARFVGLVILAATLLAAGASFLYNRGEEADR
                     ADRVAMQVNMRLRDHIAVLEGVRALYQSDSQSSGPGIRAYLASLRPQIHAPGMEGIGI
                     AVAMRQGTPAAAEAMLRQNYGRDIRVWPATSQPIGFPIVLVEPYTPRRDQALGYDMYS
                     ERIRREAMRRAWQSGQPAASGIVQLVQEKGVAKRQPGFLIYVPIYAGRAASGDAPPSA
                     AFATAPNARPIEAFVYAPFRIHDLMTAVLGSQLDTIEGLQIYAGEGPSAPLVFSRGKM
                     GWDTHEQKLRVGDRQWTMRISYGRLLERIGRPFGIFLFGFAIMLLAMQLHRLQQRRVG
                     AFQALADEQARHAADRELMIGEMAHRMKNAFARIGALARITLRESTSLEDFEARFDGR
                     MRALSDAKQMLVTGAVGSVELGQIVRRELELAGASEQQLAGIAGPHVRLGDEGAQAIS
                     LAVHEFVTNSIKYGALAGQGHLAVGWHRDDGEIELDWIESDLAETPDIESESFGTQFI
                     RTLIERQLKGSWSRTAVDKRLAIVIRWPDNGTAD"
     gene            1903..2463
                     /locus_tag="VN034_01265"
     CDS             1903..2463
                     /locus_tag="VN034_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV59256.1"
                     /translation="MIIYGSVVSPFVRKLLAYLGEKGIEFELKGVGIGDPDPGFQAAS
                     PLGKMPAMDDDGFLLADSSAIIQYVEAKHPEPPLIPADPEARGRVIWWEEFGDTVFAA
                     CSGKMFFNRIVAPKFLGREGDLAAAALADLAVASPLMNFRHCGARIDAAVHPKIAAWS
                     EAILSRPSMAPWIAKEERLIERVLAG"
     gene            complement(2547..6005)
                     /locus_tag="VN034_01270"
     CDS             complement(2547..6005)
                     /locus_tag="VN034_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59257.1"
                     /translation="MKLRYSIAASLMAIGAATVVAAPAQAQQITTGIQGQVNDDSGAA
                     IGGATVVITDTRTGAARTITTGADGRFSTTGLTTGGPYTVSVNADSYEGQSLQNIYTT
                     LQGSTDLTFALTSGGGEIVVTGSRVRVTQLEVGPGTSFSTEVLESAPSFNRDVRDIIR
                     LDPRVSLDRDDAGGGQDRISCLGGNDRGNAFTVDGISQGDIYGLNDTGFSSRSSTPIP
                     YDAVRETQVQFAPFDVDYGAFTGCAINVVTKSGTNDYRFGGFFEYSDNGLRGNSVAGE
                     PVPPIEAEKRWGVWAGGPIIKDRLFFFGAYEHQEAGQAQDDGPAGAGYANEIPGIPVE
                     AFNEISDVLSSVYGIETGPLVRSRPFSNDRYFGRLDWQVNDDHRLELTYQRLDEGSTS
                     PDDFSSSSSSPRVTGLNTFFTSGTKSDYYSGRLYSQWSDEFSTELRYSRSKITDRQDP
                     IGGGEAQSDNPIPRIIVGVTTGEPGEEIYGQVLAGPGFSRAANDLRTRVDSYRAVAKL
                     DKGAHQFKAGIEVNRVDLFNLFVQNATGTLYFEDIDALREGLLSNGDATNPSAEELAL
                     GEAVGAEGNFTATGDVNDAAAAFKRTTWSIFAQDDWEVSDRLNAVVGVRADWYSGGQP
                     NYNPVFQGRYGIPNTTAFSNLDPIIMPRLALTYDMDDFAVFGRPKITAGVGIFSGGDP
                     LVWFGNAFQNDGRGFATGDTTDAACPANIDVVVGGQFTGIPGCFQQAASDSAAAGQGD
                     TQSISPNIKMPSVLRANIGFTSELNFAPSGFFSGWNVNLDYIYSKYRDPFTIVDLSQT
                     PDIREGLDGFTVDGRPIYAAIDPNAAGCGARLVGLNPAPVFEGVTEACFDTRRDDELQ
                     LTNGPGYESHIASFILSKRFEGGLFTENGNVDFNIGYSYTDAQDRRNMYNSTAGSNYD
                     RTAAFDRQNPAASRSFFSSKHNITTRLGFREEFFDDLSTRFAISFVARSGRPYSLTFT
                     GGGVFNDTASGFENALIYLPTGADDPNIAPPIFNGAGEQIGGSDLNAVRELVAFASGL
                     GCAKKYLGRSIARNTCSNDWYFDMDLSFSQEIPGPGRLFGRNDKLKLYATMDNFLNFL
                     DQDWNVQRRRNFVGLQDIATLDGIDNQGRYIIDGFNGIDAFNDDNQINTSSSVWRLKV
                     GVSYEF"
     gene            complement(6274..7545)
                     /gene="clpX"
                     /locus_tag="VN034_01275"
     CDS             complement(6274..7545)
                     /gene="clpX"
                     /locus_tag="VN034_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWV59258.1"
                     /translation="MTKLSGSDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     NDIIREEIKGGVAARKDGAVPTPLEICQHLDAYVIGQNTAKRVLSVAVHNHYKRLANS
                     GRGDDVELAKSNILLVGPTGSGKTLLAQTLARFLDVPFTMADATTLTEAGYVGEDVEN
                     IILKLLQASDYNVEKAQRGIVYIDEIDKISRKAENPSITRDVSGEGVQQALLKLMEGT
                     TASVPPQGGRKHPQQEFLQVDTTNILFIAGGAFSGLEKIIGDRLQGKSIGFGAHVAGP
                     DERRSGEVLKQIEPEDLLKFGLIPEFVGRLPVIATLEDLDVDALVKILGEPKNALVKQ
                     YKKLFDLEEVALTFTDDALVAVAKKAIERKTGARGLRSIVEAILLDTMFDLPDLTDVV
                     EIVVDKDVVEGRKDPVRVYADKTKEAAGDAA"
     gene            complement(7805..8455)
                     /locus_tag="VN034_01280"
     CDS             complement(7805..8455)
                     /locus_tag="VN034_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWV59259.1"
                     /translation="MIDPLAALVPVVVEQTSRGERSFDIFSRLLRERIVFVTGEVEDQ
                     MASLITAQLLFLESENPKKDIYMYINSPGGVVTAGMAIHDTMQYIRPRVGTVCIGQAA
                     SMGSFLLAAGEPGMRVALTNARVMVHQPSGGARGMASDIEIQAKEILRIKKRMNDLYV
                     KYTGKPLKEIEKAMDRDTFLEADEAKEFGIVDHVYDRRPGLPGDDAPKDVSEGPTP"
     gene            complement(8815..9153)
                     /gene="fdxA"
                     /locus_tag="VN034_01285"
     CDS             complement(8815..9153)
                     /gene="fdxA"
                     /locus_tag="VN034_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HWV59260.1"
                     /translation="MTYVVTDACVRCKYMDCVEVCPVDCFYEGENMLVINPNECIDCG
                     VCEPECPAEAILPDTESGLEKWLEVNSKFSAEWPNITVKKETPADADEFKGVEDKFDK
                     FFSPEPGEGD"
BASE COUNT         1543 a         2909 c         3261 g         1765 t
ORIGIN      
        1 tgcgcgcgat cttcggagag atatttggtc gtgacgacgg cgccgtcggg gcgccgcgcg
       61 acgacatcgg tgaaggtgcc gccgcggtcg atccagatat gccagtcagt cggcggtgcc
      121 attgtcgggc cagcgaatga cgatggcgag gcgcttgtca accgcggtgc ggctccagct
      181 gcccttcaat tgccgttcga tcagggtgcg gatgaactgg gtgccgaagc tttcgctttc
      241 gatgtcgggg gtttcggcga ggtcgctttc gatccagtcg agttcgatct cgccgtcgtc
      301 gcggtgccag ccgaccgcaa gatgaccctg gcccgcgagc gcgccatatt tgatgctgtt
      361 ggtgacgaat tcgtggaccg cgagcgagat cgcctgcgcg ccctcgtcgc ccaagcggac
      421 gtgggggccg gcaatgcccg cgagctgctg ttccgacgcg ccggcgagtt cgagttcgcg
      481 gcggacgatc tgcccgagct cgacgctgcc gaccgcgccg gtgacgagca tctgtttggc
      541 gtcggacagc gcgcgcatgc ggccgtcgaa gcgggcctcg aaatcctcga ggcttgtcga
      601 ttcgcgcagc gtgatgcgcg cgagcgcgcc gatgcgcgca aaggcgttct tcatccggtg
      661 cgccatctcg ccgatcatca gctcgcggtc ggcggcgtgg cgcgcctgtt cgtcggcgag
      721 cgcctggaaa gcgccgacgc ggcgttgctg gaggcggtgg agctgcatcg cgagcagcat
      781 gatcgcgaag ccgaagagga agatgccgaa gggccggccg atccgttcga gtagtcggcc
      841 gtaggagatg cgcatcgtcc attgccggtc gccgacgcgg agtttctgtt cgtgcgtatc
      901 ccagcccatc ttgccgcggc tgaagacgag cggcgccgac gggccctcgc ccgcatagat
      961 ttgcagcccc tcgatcgtgt cgagctgcga cccgagcacc gcggtcatca ggtcgtggat
     1021 gcgaaaaggg gcgtagacga aggcctcgat cggccgcgcg ttcggggcgg tggcgaaggc
     1081 ggccgaaggg ggcgcgtcgc cggacgcggc gcggccggca tagatgggga catagatgag
     1141 gaagccgggc tgccgtttgg cgacgccctt ttcctggacg agctggacga tgccgctcgc
     1201 cgcgggctga ccgctctgcc acgcgcggcg catcgcctcg cgccggatcc gctcgctgta
     1261 catgtcataa ccgagcgcct ggtcgcggcg cggcgtatag ggttcgacga gcacgatcgg
     1321 aaatccgatc ggctggctgg tcgcgggcca gacgcgaatg tcgcgcccgt aattctggcg
     1381 cagcatcgcc tcggccgccg cgggggttcc ctgccgcatc gcgaccgcga tgccgatgcc
     1441 ttccatcccc ggcgcatgga tctgcggccg cagcgacgcg agataggcgc ggatgcccgg
     1501 gccgctcgac tggctgtccg attgatagag ggcgcgcacg ccttcgagca ccgcgatatg
     1561 atcgcgcagc cgcatattga cctgcatcgc gacgcggtcg gcgcgatcgg cctcttcgcc
     1621 gcgattgtag agaaagctcg cccccgccgc gagcagcgtg gcggccaata tgacaagacc
     1681 cacgaagcgg gcggagaacg ccagcagccg atcggcccat gaagaagggc gcattgattc
     1741 atcctgcctg atggctgccg tggcagcgcc cccctccacc gaccgatgtt ccatgccgcg
     1801 ccgccgatgg caagtttatt tcgtgccgat gcggcggtga tgaacgggtt gcgatgcgca
     1861 acctttcgcc tatgctggcg cgaggaaaaa gggagaagcg cgatgatcat ttacggatcg
     1921 gtggtgtcgc cgttcgtgcg caagctgctc gcctatctgg gcgagaaggg gatcgagttc
     1981 gagctgaagg gcgtcggtat cggcgacccc gatccggggt ttcaggccgc atcgccgctc
     2041 ggcaagatgc ccgcgatgga cgacgacggc ttcctgctcg ccgattcgag cgcgatcatc
     2101 caatatgtcg aggcgaagca tcccgagccg cctctgatcc ccgccgaccc cgaggcgcgg
     2161 gggcgcgtga tctggtggga ggaattcggc gacacggtgt tcgcggcgtg cagcggcaag
     2221 atgttcttca accgcatcgt cgcccccaaa tttctggggc gcgagggcga tctcgcggcg
     2281 gcggcgctcg ccgatctggc ggtggcgtcg ccgctgatga atttccgcca ttgcggcgcg
     2341 cgcatcgacg cggcggtgca tccgaagatc gcggcgtgga gcgaggcgat attgtcgcgg
     2401 ccgagcatgg cgccgtggat cgccaaggaa gagcggctga tcgagcgcgt gctggcgggt
     2461 tgaggttgga gggcggccaa tcgccgccgt agaaaaaggg cgcgggagtt gcctcccgcg
     2521 cccgatttta ttgctctgac ctgtgatcag aattcgtacg acacgccgac cttcagacgc
     2581 cacaccgacg agctggtgtt gatctggttg tcgtcgttga acgcatcgat gccgttgaag
     2641 ccgtcgatga tgtagcggcc ctgattgtcg atcccgtcga gcgtcgcgat atcctgaagc
     2701 cccacgaaat tgcggcggcg ctggacgttc caatcctggt cgaggaagtt caggaaattg
     2761 tccatcgtcg catagagctt cagcttgtcg ttgcggccga acaggcgccc cggacccgga
     2821 atttcctgcg agaaggacag gtccatgtcg aaataccagt cgttcgagca ggtgttgcgc
     2881 gcgatcgaac ggccgagata tttcttggcg cagccgaggc cgctggcgaa ggccacgagt
     2941 tcgcgcaccg cattgagatc cgatccgccg atctgctcgc cggctccgtt gaaaatcggc
     3001 ggcgcgatgt tcggatcgtc ggcgcccgtc ggcagataga tcagcgcatt ttcgaagccc
     3061 gacgcggtgt cgttgaacac gccgccgccg gtgaaggtca ggctgtacgg ccggcccgag
     3121 cgcgcgacga agctgatcgc gaagcgcgtc gacagatcgt cgaagaattc ctcgcggaag
     3181 ccgaggcgcg tcgtgatatt atgcttgctg ctgaagaagc tgcgcgacgc cgccgggttc
     3241 tggcggtcga aggcggcggt ccggtcatag ttcgaccccg cggtcgaatt atacatgttc
     3301 cgccggtcct gcgcgtcggt gtagctatag ccgatattga aatcgacatt gccgttctcg
     3361 gtgaacagcc caccctcgaa acgcttcgac aggatgaacg aggcgatgtg gctttcatag
     3421 cccggaccgt tggtcagctg cagctcgtcg tcgcgcctgg tgtcgaagca ggcctcggtg
     3481 acgccttcga agaccggcgc ggggttgagc ccgacgagcc gcgcgccgca gccggcggcg
     3541 ttcggatcga tcgccgcgta gatcgggcgc ccgtcgaccg tgaaaccgtc gagcccctcg
     3601 cggatgtcgg gggtctgcga cagatcgacg atggtgaacg gatcgcgata tttgctgtag
     3661 atatagtcga ggttgacgtt ccagccgctg aagaagcccg agggggcgaa attcagctcc
     3721 gacgtgaagc cgatattggc gcgcagcacc gacggcatct tgatgttcgg actgatcgac
     3781 tgggtatcgc cctgacccgc cgccgcgctg tccgacgccg cctgctggaa gcagcccgga
     3841 atgccggtga actggccgcc cacgacgacg tcgatattgg ccgggcaagc ggcgtcggtc
     3901 gtgtcgccgg tggcgaagcc gcgcccgtcg ttctggaacg cgttgccgaa ccagacgagc
     3961 gggtcgccgc ccgagaagat gccgacgccg gcggtgatct tgggccggcc gaacaccgcg
     4021 aaatcgtcca tgtcataggt cagcgcgagg cgcggcatga tgatcgggtc gaggttgctg
     4081 aacgccgtcg tgttggggat gccgtagcgg ccctggaaca cggggttgta attgggctgg
     4141 ccgcccgaat accagtcggc gcggacgccg accacggcat tcagccggtc gctgacctcc
     4201 caatcgtcct gcgcgaagat cgaccaggtc gtgcgcttga acgccgccgc ggcgtcgttg
     4261 acgtcgccgg tggcggtgaa attgccttcg gcgccgaccg cctcgcccaa ggcgagctcc
     4321 tcggccgagg ggttggttgc gtcgccgttc gacaacaggc cttcgcgcag cgcgtcgatg
     4381 tcctcgaaat agagcgtgcc ggtcgcgttc tggacgaaca ggttgaacag gtcgacgcgg
     4441 ttcacctcga tcccggcctt gaactggtgc gcgcccttgt cgagctttgc gacggcgcgg
     4501 taggaatcga cgcgggtgcg caggtcgttc gcggcgcgcg agaaacccgg tccggcgaga
     4561 acctggccgt aaatctcttc accgggctcg ccggtggtga cgccgacgat gatgcgcggg
     4621 atcggattgt ccgactgcgc ctcgccgccg ccgatcggat cctggcggtc ggtgatcttc
     4681 gaacgcgaat agcgcagctc ggtcgagaat tcgtcgctcc attgcgaata gagacgtccc
     4741 gaataatagt ccgacttggt gccgctggtg aagaaggtgt tgagcccggt gacgcgcggg
     4801 ctgctgctgc tgctgctgaa atcgtcgggg ctggtcgagc cttcgtcgag ccgctgatag
     4861 gtcagctcga gccggtggtc gtcgttgacc tgccagtcga ggcggccgaa atagcggtcg
     4921 ttcgaaaagg gacggctgcg gaccagcggg cccgtttcga tgccatagac gctgctcagc
     4981 acgtcggaga tttcgttgaa ggcttcgacc ggaatgccgg ggatttcgtt ggcatagcct
     5041 gcgcccgccg gaccgtcgtc ctgcgcctga ccggcttcct gatgttcata ggcgccgaag
     5101 aagaacaggc gatccttgat gatcgggccg ccggcccaca cgccccagcg cttttcggct
     5161 tcgatcggcg ggaccggctc gcccgcgacg ctgttaccgc gcaggccatt gtccgaatat
     5221 tcgaagaagc cgccgaaacg ataatcgttg gtgcccgatt tggtgacgac gttgatcgcg
     5281 cagccggtga aggcgccgta atcgacgtcg aacggcgcga actggacctg cgtttcgcga
     5341 accgcgtcat aggggatcgg ggtcgagctg cgcgacgaga agccggtgtc gttgaggccg
     5401 tagatgtcgc cctggctgat gccgtcgacg gtgaaggcgt tgccgcggtc gttgccgccg
     5461 aggcacgaga tgcggtcctg gccgccgccg gcatcgtcgc ggtcgaggct gacgcgcggg
     5521 tcgaggcgga tgatgtcgcg gacgtcgcgg ttgaacgagg gcgcgctttc gagcacttcg
     5581 gtcgagaagc tggtgcccgg gccgacttcg agctgcgtca cgcgcacgcg gctgccggtg
     5641 acgacgatct cgccgccgcc cgaggtgagc gcgaaggtga ggtcggtgct gccctgcagc
     5701 gtggtgtaga tgttctggag cgactggcct tcatagctgt cggcgttgac gctgaccgtg
     5761 taagggccgc cggtggtgag gccggtggtc gagaagcggc cgtcggcgcc cgtggtgatc
     5821 gtgcgcgcgg cgccggtgcg ggtgtcggtg atgacgacgg tcgcgccgcc gatcgcggcg
     5881 ccgctgtcgt cgttgacctg gccctggatg ccggtcgtga tctgctgcgc ctgggcgggc
     5941 gcggcgacga cggtcgcggc gccgatagcc atcaggctag ccgcgatcga ataacgaagt
     6001 ttcatgtcgg aatgtcccct attggtgcgt tcggcaggca cgcgatcggg gcgcgccatg
     6061 acctgcgagc gcccctgaag cccgcatgtg gcacgattgt gacagcaatt gtgacagtgc
     6121 aagccgcgtg cggcgggcgg tgggggaggg ggtaagaaaa gcccgaattt ccgtcgcgcg
     6181 cccgatttta tccacaggcg cggtctcgtt tttccgggct actgttgctg caatgcaaca
     6241 taagccgcgc gccgcacggg cgcgcgactc ggatcaggcg gcgtcgccgg ctgcttcctt
     6301 ggttttgtcg gcgtagacgc ggacggggtc cttgcggcct tcgacgacat ccttgtcgac
     6361 gacgatctcg accacgtcgg tgaggtcggg caggtcgaac atcgtatcga gcaggatcgc
     6421 ctcgacgatc gagcgcagcc cgcgcgcgcc ggtcttgcgt tcgatcgcct tcttggcgac
     6481 cgcgacgagt gcatcgtcgg tgaaggtcag cgcgacctct tcgaggtcga acagcttctt
     6541 atactgcttg accagtgcgt tcttgggttc gccgaggatc ttgaccagcg cgtcgacgtc
     6601 gagatcctcg agcgtcgcga tgacgggcag gcggccgacg aattcgggga tcaggccgaa
     6661 tttgaggaga tcctcaggct cgatctgctt caatacttcg ccgctgcggc gctcgtcggg
     6721 gccggcgaca tgcgcgccga aaccgatcga cttgccctgg aggcggtcgc cgatgatctt
     6781 ttcgaggccc gagaaggcgc cgcccgcgat gaacaggatg tttgtcgtat cgacctgcag
     6841 gaattcctgc tgcggatgct tgcgcccgcc ctgcggcggc accgacgcgg tggtgccttc
     6901 catcagcttg agcagcgcct gctgcacgcc ttcgcccgac acgtcgcgcg tgatcgacgg
     6961 gttctccgcc ttgcggctga tcttgtcgat ctcgtcgata tagacgatgc cgcgctgcgc
     7021 cttttcgaca ttatagtcgc tggcttgcaa gagcttgagg atgatgttct cgacgtcctc
     7081 gccgacatag cccgcctcgg tcagcgtcgt cgcgtcggcc atggtgaagg gcacgtcgag
     7141 gaagcgtgcg agcgtctgcg cgagcagcgt tttgccgctg ccggtcgggc cgacgagcag
     7201 gatgttcgac ttcgcgagtt cgacatcgtc gccgcggccg ctgttcgcga ggcgcttgta
     7261 atggttgtgc accgcgaccg acagcacgcg cttggcggtg ttctggccga tgacataggc
     7321 gtcgagatgc tggcagattt cgagcggcgt gggtaccgcg ccgtccttgc gcgcggcgac
     7381 accgcccttg atctcttcgc ggatgatgtc gttgcacagc tcgacgcatt cgtcgcagat
     7441 gaacacggtc ggcccggcaa tcagcttgcg gacttcgtgc tgcgacttgc cgcagaagga
     7501 gcaatagagg gtgctcttgc tgtccgagcc actcaatttc gtcataaatc ttcctatggc
     7561 gggcccaaaa ggggcgcgcc gtccttatcc tagcccccgg tcacccaatt acaatatggc
     7621 aattgggtga cacgggttca atgtcccgcc tagcgccaag gcgccaagcg gatgtcaaca
     7681 aacgcggtta ccgcttgtct ttcgttggta ggcggcgccg gcccgctccc cctcccagcc
     7741 tcccgacatg gtatcctatg ggaggctggg agggggagtg ggccggcgcc gtaacactac
     7801 caaatcaagg cgtcgggccc tcgctcacat ccttcggcgc gtcgtcgccg ggcaggcccg
     7861 ggcggcggtc gtagacatgg tcgacgatgc cgaattcctt ggcttcgtcg gcttcgagga
     7921 aggtgtcgcg gtccatcgcc ttctcgatct ctttcaaggg cttgccggta tatttgacgt
     7981 agaggtcgtt catccgcttc ttgatgcgca ggatttcctt ggcctggatc tcgatgtccg
     8041 acgccatgcc gcgcgcgccg cccgacggct ggtggaccat cacgcgcgcg ttggtcagcg
     8101 cgacgcgcat cccgggctcg cccgccgcga gcaggaagct gcccatcgac gcggcctggc
     8161 cgatgcagac ggtgccgacg cgcgggcgga tatattgcat cgtgtcatgg atcgccatgc
     8221 ccgccgtgac gacgccgccc ggcgagttga tgtacatata gatgtccttc ttcggatttt
     8281 ccgattcgag gaacaggagc tgggcggtga tcagcgaggc catctggtcc tcgacctcgc
     8341 cggtgacgaa gacgatccgt tcgcgcagca gacgcgagaa aatgtcgaag ctgcgttcgc
     8401 cgcggctcgt ctgctcgacg acgacgggaa caagggcggc gagcgggtcg atcattggga
     8461 attcgtcctt cattatgcgg gttgccgcgc cgaggaccgg cgggctcccc cttccgcgac
     8521 ctacatcggc ggcaaggcgg agtgtttcaa gagggcaaat gacgccgtgc ggctggcggt
     8581 ggcctcttgg agcaagcgga cgttgcccga tcctccctgt cgcgcagcga tggggagggg
     8641 gaccgttcgc gaagcgaatg gtggaggggc ggcgacctca cgccattggc ccctccgtca
     8701 gcgcttcgcg ctgccacctc cccatgcctg cggcacaggg aggatcgaag aacgttcgca
     8761 ccgccaaaaa gaaggggcgg gaaagtcccg ccccattgtc tcgtatcgaa gcgatcagtc
     8821 gccttcgccc ggttcgggcg agaaaaattt gtcgaacttg tcctcgacgc ccttgaattc
     8881 gtcggcgtcg gcgggcgttt ccttcttgac cgtgatgttc ggccattcgg cgctgaactt
     8941 gctgttcacc tcgagccact tttcgaggcc gctctcggtg tcgggcagga tcgcctcggc
     9001 ggggcattcg ggttcgcaca cgccgcagtc gatgcactcg ttcggattga tgacgagcat
     9061 attctcgccc tcatagaaac agtcgacggg gcacacctcg acgcagtcca tatatttgca
     9121 ccggacgcag gcatcggtga cgacataggt catgggtatt cgctcccttg gtcggcggca
     9181 cccttgccgt ccggacaatg aatgcccccg cctctaagcc gcgcgaatgt tacgcgtcaa
     9241 cgcaaagcga cagttgcgaa tcactctcac tcgataggca aaatcatgcg gttccggggg
     9301 acagcttttc ctcacccgcc gatagttcag ctggaggggg cggggtccgg cgaatcgagc
     9361 cggacatagc aggcctgcgc ttcgggcgcc gggccgcggc gcaagggcag cgtcagaagg
     9421 cggatcacct cgatgcgctc gccgaccggc agcaccaatg tcgcgccggc gtggaccg
//
LOCUS       DATMSU010000050         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_67373, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000050 DATMSU010000000
VERSION     DATMSU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67373"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VN034_01290"
     CDS             <1..299
                     /locus_tag="VN034_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS sensor protein"
                     /protein_id="HWV59261.1"
                     /translation="HARISVRETVEAANPMLTALVGVDPAAIRRVRFSALLAVGQRAA
                     FVEALESVFRSGDPARVASQIVTREGSAVDVILTIAEVRGPYASEGAVVLVTRP"
     gene            490..1683
                     /locus_tag="VN034_01295"
     CDS             490..1683
                     /locus_tag="VN034_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV59262.1"
                     /translation="MDMEFSPEDLAFQQEVRQFIAENYPAELRGKQDEGEELSKEDFL
                     AWHKILYKKGWIAPAWPVEYGGTGWTPTQRFIWSEETARADCIRLMPFGLAMVGPVIY
                     TFGTPEQKAHFLPRILSGEDWWCQGYSEPGSGSDLASLRTTAVRDGDDYIVNGQKTWT
                     TLAQHADWGFFLVRTDKDAKQQEGISFLLIDMKSPGITVRPIITLGGEHEVNEVWLED
                     VRVPQSQRVYEENKGWTCAKFLLAHERTGIAGVAASKRGIEKVKAIARTELDGDKPLL
                     ANPFFKRKVAELEVDLTALEFTELRSLAGANAGKGPGPESSLLKIKGSEIQQRLTELT
                     LEAVGHYGAPYFRGFGEGDNEHPIGPDYAHRAAPTYFNMRKTTIYGGSNEIQRNIIAK
                     MVLGL"
BASE COUNT          343 a          564 c          546 g          298 t
ORIGIN      
        1 gtcacgcccg catttcggtc cgcgaaacgg tcgaggcggc gaacccgatg ctcaccgcgc
       61 tggtcggcgt cgatcccgcc gcgatccgcc gcgtgcgctt ttccgcgctt ctcgcggtcg
      121 ggcagcgcgc cgccttcgtc gaggcgctcg aatcggtttt ccgttcgggc gatcccgccc
      181 gcgtcgcgtc gcagatcgtg acgcgcgaag gatcggccgt cgacgtcata ttgacgatcg
      241 ccgaagtgcg cggcccctat gccagcgagg gcgcggtcgt gctcgtcacg cgcccctagc
      301 acccgcgccc ccattcgatt gccatcatgc ctggccgacg ctgatcggcc cgcgacggtt
      361 gccgatgctg gacaagccgg aaggttaggc gcaatcatcg atgcaaggaa ggttgcaagt
      421 cgcaacttgc cgtttacgta aaggtgagcg cccattataa gggcgccaat gcccaatggg
      481 agattcgtga tggacatgga attcagcccc gaggatttgg ccttccagca ggaagttcgc
      541 caattcatcg ccgaaaatta ccccgcggaa ctccgcggca agcaggatga gggcgaagaa
      601 ttgtccaagg aggacttcct cgcctggcac aagattctgt acaagaaagg ctggatcgcg
      661 cccgcctggc cggtcgaata tggcggcacc ggctggacgc cgacgcagcg tttcatctgg
      721 tcggaagaga cggcgcgcgc tgactgcatc cgcctgatgc ccttcggcct cgcgatggtc
      781 gggccggtca tctacacctt cggtaccccc gaacagaagg cgcatttcct gccgcgcatc
      841 ctctcgggcg aggattggtg gtgccagggc tattcggaac ccggttcggg cagcgacctt
      901 gcttcgctcc gcaccacggc cgtgcgcgac ggcgacgact atatcgtcaa cgggcaaaag
      961 acctggacga cgctcgcgca gcacgccgac tggggctttt tccttgtccg taccgacaag
     1021 gacgccaagc agcaggaagg catcagcttc cttttgatcg acatgaaatc gcccggcatc
     1081 accgtgcgcc cgatcatcac gctgggcggc gagcatgagg tcaacgaggt gtggctggag
     1141 gacgttcgcg tgccgcaatc gcagcgcgtc tatgaagaga ataagggctg gacctgcgcc
     1201 aaattcctgc tcgcgcacga acgcaccggc atcgccggcg tcgcggcgtc gaagcgcggg
     1261 atcgagaagg tgaaggcaat cgcgcgcacc gaactcgacg gcgacaagcc cttgctcgcc
     1321 aatcccttct tcaagcgcaa agtcgccgaa ctggaagtcg atctcaccgc gctcgaattc
     1381 accgaactgc gcagcctcgc cggcgcgaat gcgggcaagg gaccgggacc ggaatcgagc
     1441 ctgctcaaga tcaagggctc ggaaatccag cagcgcctca ccgaactgac gctcgaagcc
     1501 gtcggccact atggcgctcc ttactttcgc ggcttcggag agggcgacaa cgagcatccg
     1561 atagggcccg attacgcgca ccgcgccgcg ccgacctatt tcaacatgcg caagacgacc
     1621 atctatggcg gatcgaacga gattcagcgc aacatcatcg cgaagatggt tttgggattg
     1681 tgacacgcgt cgcccccgcg aaggcggggg ccgctggagg cccggtctcc cggtcaatgt
     1741 aaaactgaag c
//
LOCUS       DATMSU010000051         5308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_86400, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000051 DATMSU010000000
VERSION     DATMSU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86400"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VN034_01300"
     CDS             <1..548
                     /locus_tag="VN034_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWV59263.1"
                     /translation="LASPRDARGLEILAAIFNDRLFDRLRAEQGASYGPVVDSHWPTG
                     FDSGGYLLVGSLLAPKDLDRFYAIAKEIAADLVTKPVSADELARNAGPIREQVARAST
                     GNVYWMYLLEGATRDPRVAAAALSIQDDISAVTAADVQRLARQYLAPERQWSLAILPK
                     GMTLADASALNTSPAPVTGGR"
     gene            complement(545..2329)
                     /locus_tag="VN034_01305"
     CDS             complement(545..2329)
                     /locus_tag="VN034_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWV59264.1"
                     /translation="MVSETETSAIGNALVVLGAAGIVIPAFARFRISPVIGFILVGLL
                     VGPSGLGALATDYPWLKHVTIASTEDIALFGEFGIILLLFSIGLELSFRRLWQLRKLV
                     FGIGAAELFIAGAILGTALLFLGNLSTPAAYGLGIALALSSTALVLPIAGTKSAVGRA
                     SFSMLLFEDLALVPIIFVLAALSPTASGDSTELMLTTLWQGGLVVAALAVGGWFLLPK
                     IFAQAARAKDPELFLAASLLVVIVAALATAAVGLSPIVGALLAGLMIAETEYHGEVEA
                     ITAPFKGLALGVFLISVGMGLNLKTIASQWPALVVAVVGVVLVKAVVTAALLRFSGAA
                     RRGTAAEVGLLMASPSETTLIVLTTALQAQLISRATAEFWQLVTAIGLTITPLLARIG
                     HDIARRIEMRSGDTQADETPEGRTVIVGFGRVGHTVAELLREHDRPYIAVDADIDTVA
                     AARRDGFSVLFADVARAGSLDRLGIESANAIVLTMDDPVQQLRMTRQLKQKYPDLPVI
                     SRARDADHAAALYQAGANDAVPETLESSLQLAEAVLIDLGVAMGPVIASIHDIRAKMR
                     HDIMAKGKLEHEPRIRRTRRHSAGGENA"
     gene            complement(2396..3610)
                     /locus_tag="VN034_01310"
     CDS             complement(2396..3610)
                     /locus_tag="VN034_01310"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWV59265.1"
                     /translation="MGKIKVANPVVELDGDEMTRIIWQWIRERLILPYLDVDLHYYDL
                     GIEERDRTDDKITVDAANAIKKYGVGVKCATITPDEARVEEFGLKKMWKSPNGTIRNI
                     LGGVVFREPIVMKNVPRLVPGWTDPIVVGRHAFGDQYKATDFRVPGPGKLRLVFEGED
                     GTLIDEEVFQFPSSGVAMGMYNLDDSIRDFARASLNYGLAREWPVYLSTKNTILKAYD
                     GRFKDLFEEVFQAEFKAQFDALGIVYEHRLIDDMVASALKWSGKFVWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLMTPDGKTVESEAAHGTVTRHYRMHQQGKATSTNPIASIFAW
                     TGGLKHRGKLDDNAALVQFADDLEKVCVATVESGKMTKDLALLIGPDQNWLTTEGFFE
                     AIVENLDKKMGG"
     gene            3772..4509
                     /locus_tag="VN034_01315"
                     /pseudo
     CDS             3772..4509
                     /locus_tag="VN034_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041383237.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
     gene            4512..5300
                     /locus_tag="VN034_01320"
     CDS             4512..5300
                     /locus_tag="VN034_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HWV59266.1"
                     /translation="MAEEAHAPLDHVPGDAEHRRIGGIPLRAFAPNAITLLALCSGLT
                     GVRFAIDGDWAAAITMIIVAGVLDGMDGRIARLLRAQSKFGAELDSLSDVIAFGVAPA
                     MILFFWSLNAAPKFGWTAALALAVCCALRLARFNSRMDAEFQPHKSAGFNTGIPAPAG
                     AGLSFVPIYLWLTTGNDLFREWYVVMPWALGIAMLMISAIPTYSWSSIRLRRSWRLFA
                     LAGVGLLGAALVTAPWLTLLAVSALYAATLPFGLASYAKVRRRG"
BASE COUNT          864 a         1733 c         1760 g          951 t
ORIGIN      
        1 gtcttgccag cccgcgcgac gcgcgcgggc tcgagatcct cgcggcgatc ttcaatgacc
       61 ggctgttcga tcgcctccgc gccgaacaag gcgcaagcta tggccccgtc gtcgacagcc
      121 actggccgac cggcttcgac agcggcggct atctgctcgt cggcagcttg ctcgcgccga
      181 aggatctcga tcgcttctat gccattgcga aggagattgc cgccgacctg gtgacgaaac
      241 cggtcagcgc cgacgaactg gcgcgcaacg ccgggccgat ccgcgaacag gtcgcgcgcg
      301 cctcgacggg caatgtctat tggatgtacc tgctcgaagg cgcgacgcgc gacccgcgcg
      361 tcgccgcggc ggcgctgtcg atccaggacg atatttcggc ggtgaccgcc gccgacgtcc
      421 agcgactcgc acgccagtat ctggcgcccg agcggcaatg gtcgctggcg atcctgccca
      481 agggaatgac gctggccgac gcgtcggcgc tgaacacctc gcccgcgccg gtgaccggag
      541 ggcgctaggc gttttcgccg ccggcggagt gacggcgcgt ccgcctgatc cgcggctcat
      601 gctcaagctt gcctttggcc atgatgtcgt ggcgcatctt ggcgcggatg tcgtggatcg
      661 acgcgatcac cggccccatt gcgacgccca ggtcgatcag caccgcttcg gccagttgca
      721 gcgagctttc gagcgtttcg ggcaccgcat cgttcgcgcc cgcctgataa agcgccgcgg
      781 catggtcggc atcgcgcgcg cggctgatca ccggaagatc gggatatttc tgcttcagct
      841 ggcgtgtcat gcgcagctgc tgcaccggat cgtccatcgt cagcacgatc gcattggcgc
      901 tttcgatgcc cagccggtcg aggcttccgg cgcgtgcgac gtcggcgaag agcaccgaaa
      961 agccgtcgcg ccgagccgcg gcgaccgtgt cgatatcggc gtcgaccgcg atatagggcc
     1021 ggtcatgctc gcgtagtagc tcggcgacgg tgtggccgac gcggccgaag ccgacgatga
     1081 ccgtgcggcc ctcgggcgtc tcgtcggcct gcgtatcgcc gctgcgcatc tcgatccgcc
     1141 gcgcgatgtc gtggccgatg cgcgcgagca aaggcgtgat cgtcaacccg atcgcggtga
     1201 cgagctgcca aaattcggcg gtggcgcggc tgatcagctg cgcctgcagc gcggtggtca
     1261 acacgatcag cgtcgtctcc gacggactcg ccatgagcag cccgacctcg gcggccgttc
     1321 cgcgccgcgc cgcccccgaa aaacgcagca acgcggcggt aacgacggcc ttgaccagaa
     1381 cgacgccgac gacggccacc accagcgccg gccactggct ggcgatcgtc ttgagattga
     1441 gccccatgcc gacgctgatc aggaacacgc cgagcgcaag ccctttgaac ggtgcggtga
     1501 tcgcctcgac ctcgccatga tattcggttt cggcgatcat caggccggcg agcagtgcgc
     1561 cgacgatcgg cgacagcccg accgccgccg tcgccagcgc ggcgacgatg acgacgagca
     1621 ggctcgccgc gagaaagagt tcggggtcct tcgcgcgcgc cgcctgcgca aagatctttg
     1681 gcagcaggaa ccagccgccg accgcgagcg ccgcgacaac gagcccgccc tgccacagcg
     1741 tcgtcagcat caattcggtg ctgtcgcccg atgccgtcgg cgacagcgcg gcaagaacga
     1801 agatgatcgg gacgagcgcc agatcctcga acaacagcat cgagaaggac gcgcgcccga
     1861 ccgccgattt cgtgcccgcg atcggcagca cgagtgccgt cgacgacagc gcgagcgcaa
     1921 tgccgagacc ataggcggcc ggggtcgaaa gattgccgag gaacaacagg gcggtgccga
     1981 ggatcgcgcc cgcgatgaaa agctccgccg cgccgatccc gaacacgagc tttcggagtt
     2041 gccacaggcg gcggaacgac agttcgagcc cgatcgagaa gagcagcagg atgatcccga
     2101 actcgccgaa cagcgcgata tcctcggtcg aggcgatcgt gacatgtttg agccatggat
     2161 aatcggtggc gagcgcgccg agccccgatg gtccgacgag caggccgacg aggatgaagc
     2221 cgatcaccgg cgaaatccgg aagcgcgcga aggccgggat gacgatgccc gccgcgccga
     2281 gcaccaccag cgcgttgccg atggcggagg tttctgtttc ggataccata agggccgacc
     2341 ttatgcaggc cggccccgat tggctagagg actatcccaa cctttttggt tgttttcagc
     2401 cacccatctt cttgtcgagg ttttcgacga tcgcctcgaa gaagccttcg gtggtcagcc
     2461 agttctggtc tggaccgatc aagagcgcga gatccttggt catcttgccg ctttcgacgg
     2521 tcgcgacgca gaccttttcg aggtcgtcgg cgaactgcac gagcgcggca ttgtcgtcga
     2581 gcttgccgcg gtgcttgagg ccgccggtcc aggcaaagat cgacgcgatc gggttggtcg
     2641 aggtcgcctt gccctgctgg tgcatgcggt aatggcgggt gacggtgccg tgcgcggctt
     2701 cggattcgac ggtcttgccg tcgggggtca tcagcacgct ggtcatcagg ccaagcgagc
     2761 cgaagccctg cgcgacggtg tccgactgaa cgtcgccgtc gtaattcttg caggcccaga
     2821 cgaacttgcc cgaccatttg agcgccgagg cgaccatgtc gtcgatcagg cggtgttcgt
     2881 agacgatgcc gagcgcatcg aactgcgcct tgaattcggc ctggaacact tcttcgaaca
     2941 gatccttgaa gcggccgtca taggctttca ggatcgtgtt cttggtcgac agatacaccg
     3001 gccattcgcg cgcgaggcca tagttcaggc tggcgcgggc gaagtcgcgg atcgaatcgt
     3061 cgaggttgta catgcccatc gcgacgcccg acgacgggaa ctggaacact tcctcgtcga
     3121 tcagcgtgcc gtcttcgcct tcgaaaacga ggcgcagctt gccggggccg gggacgcgga
     3181 agtcggtcgc cttatactgg tcgccgaacg cgtggcgccc gacgacgatg gggtcggtcc
     3241 agccggggac gaggcgcggg acgttcttca tcacgatcgg ttcgcggaag acgacgccgc
     3301 ccaggatgtt gcggatcgtg ccgttgggcg atttccacat ctttttcagg ccgaattcct
     3361 cgacgcgcgc ttcgtcgggg gtgatcgtcg cgcacttcac gccgacgcca tatttcttga
     3421 tcgcgttcgc ggcatcgacg gtgatcttgt cgtcggtgcg gtcgcgttcc tcgatgccga
     3481 ggtcgtaata atggagatcg acgtcgagat aggggaggat cagccgctcg cggatccact
     3541 gccagatgat ccgggtcatt tcgtcgccgt cgagttcgac gaccggattg gctaccttga
     3601 tcttgcccat gctttcagcc tttttcctag gggagtcgga gttgcagcgg gccttaggca
     3661 aagcgggacg attgatcaac cgcccgtcgt gcgtccccac ggtaccggac ggcaacgatc
     3721 gggccgcctt atccattgtc agtatagaag ccgcccccta gaagaatggt catgtccata
     3781 atcatctcat cgaaccggcc cggtggcggc atcaaatggc aatggccctc ggttcatccc
     3841 gaaggccgga aattcctgct gatctcgggc atcataaccc tctgtctgtg gctgctgcct
     3901 atctggagcc tgttcggctg gctgatgctc ggcgtgacaa tctgggtcgg cgccttcttc
     3961 cgcgacccgg tgcgcatcac cccggcgagc agcgatctgg tcgtcgcgcc cgccgacggg
     4021 ctgatcaccc agatcgccga agttccgccg ccgcccgaaa tcgcgggtcc cgacgggctc
     4081 ggcgccgcgc cgatgctgcg cgtctccatt ttcatgagcg tcttcgacgt ccacatcaac
     4141 cgcacccccg tcgcgggcgc gttgcgcaag ctcgtctata tccccggcaa attcgtgaac
     4201 gccgacctcg acaaggcgag cgaggagaat gagcgctagc atttcgtcgt cgagcgtgcc
     4261 gacggcgtgc gcatcggctt cacccagatc gcggggctcg tcgcgcggcg catcatgccc
     4321 ttcgtgtcga tgggcaatga actcgccacc ggccagcgcg tcggcctgat ccggttcggc
     4381 agccgcgtcg acgtgtacct gcccgcgggc accgcgccgc aggtgctgct cggccagcgc
     4441 acgctggcgg gcgagacggt gatcgcgcgg atcggcattg ccgagacgat cgaaggcgtc
     4501 gggcaatagg gatggcggag gaggcccacg ccccgctcga tcatgtcccg ggcgatgccg
     4561 agcatcgccg catcggcggg attccgctgc gcgccttcgc gcccaatgcg atcacgctgc
     4621 tggcgctttg ttccgggctg accggggtcc gttttgcgat cgacggcgat tgggcggcgg
     4681 cgataacgat gatcatcgtc gccggggtgc tcgacgggat ggacgggcgg atcgcgcggc
     4741 tgctccgcgc gcaaagcaag ttcggcgccg aactcgattc gctctccgac gtgatcgcct
     4801 ttggcgtcgc gccggcgatg atcctgtttt tctggtcgct caacgcggcg ccgaaattcg
     4861 gctggaccgc cgcgctcgcg ctcgcggtct gctgcgcgct tcggctcgcg cgtttcaatt
     4921 cgcgcatgga tgccgagttt cagccgcaca agtcggcggg cttcaacacc ggcattcccg
     4981 caccggcggg ggcggggctg tcgttcgtgc ccatctatct gtggctgacg acgggcaacg
     5041 acctgtttcg cgaatggtat gtcgtgatgc cgtgggcgct cggcatcgcg atgctgatga
     5101 tctcggcgat cccgacctac agctggtcgt cgatccggct gcgccggtcg tggcgcctgt
     5161 tcgccttggc gggggtcggc ctgctcggcg ctgcgctcgt caccgcgccc tggctgaccc
     5221 tgcttgcggt ctcggcgctc tatgccgcga cgctgccttt cggcctcgcg agctatgcga
     5281 aggtcaggcg gcgcggctga ttgccggc
//
LOCUS       DATMSU010000052         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_114816,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000052 DATMSU010000000
VERSION     DATMSU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114816"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /gene="eno"
                     /locus_tag="VN034_01325"
     CDS             <1..640
                     /gene="eno"
                     /locus_tag="VN034_01325"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833573.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWV59267.1"
                     /translation="ASTRAALDFIAASVDQAGFKLGTDVVLALDCAATEFFKNGKYEI
                     SGEGLSLSPEQMAEYLAALVKDYPIKSIEDGMSEDDFTGWKALTDLVGDKCQLVGDDL
                     FVTNPARLEQGIKDGLANSLLVKVNQIGTLSETLDAVDMAHRARYTAVMSHRSGETED
                     STIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGDMARYPGASIFG"
     gene            710..1036
                     /locus_tag="VN034_01330"
     CDS             710..1036
                     /locus_tag="VN034_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWV59268.1"
                     /translation="MTQRHKFRKSMSRATGPAIAVMVVLAMIGYIIFGPTGLYAWGEY
                     GQSVEKKRVILSELTKKQKELQNRVNLLDRGRVDPDLAEEYVREKLGAYHPDEYIIPM
                     EPDAKP"
     gene            1292..2362
                     /gene="pdhA"
                     /locus_tag="VN034_01335"
     CDS             1292..2362
                     /gene="pdhA"
                     /locus_tag="VN034_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWV59269.1"
                     /translation="MAKAPARKSAAPKKTATTPAAATNREQPRDPAPYDASPEELEKF
                     YREMLLIRRFEEKAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALDGDKDSVITGYRDH
                     GHMLAYGIDPKVIMAELTGRQAGISKGKGGSMHMFSVEHKFYGGHGIVGAQVSLGTGL
                     AFAHKYRGDGGVAMAYFGDGAANQGQVYESFNMAELWKLPIIFVIENNQYAMGTSVNR
                     ASAEDQLYRRGESFRIPGMQVDGMDVLAVRGAAEAALEWVRAGKGPVLMELKTYRYRG
                     HSMSDPAKYRSREEVQAVRDKSDAIEHLKKLMTDAGVSEDKIKDIDKEIRAVVAESAD
                     FAESAPEPELSELYTDVLVEQY"
     gene            2364..>2641
                     /locus_tag="VN034_01340"
     CDS             2364..>2641
                     /locus_tag="VN034_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWV59270.1"
                     /translation="MAIELKMPALSPTMEEGTLAKWLVKEGDTVKPGDILAEIETDKA
                     TMEFEAIDEGTIGQILVAEGTDNVKVGTVIATITGEGEEAAAPAAAPAP"
BASE COUNT          555 a          828 c          844 g          414 t
ORIGIN      
        1 cgcctcgacg cgcgccgcat tggacttcat cgccgcgtcg gtcgatcagg cgggcttcaa
       61 gctcggcacc gacgtcgtgc tcgcgctcga ttgcgccgcg accgaattct tcaagaacgg
      121 caaatatgag atcagcggcg aggggctgtc gctcagcccc gagcagatgg ccgaatatct
      181 cgccgcgctc gtcaaggatt acccgatcaa gtcgatcgag gacgggatga gcgaagatga
      241 tttcaccggc tggaaggcgc tcaccgacct tgtcggcgac aagtgccagc tcgtcggcga
      301 cgacctgttc gtcaccaacc cggcgcggct ggagcagggc atcaaggacg gcctcgccaa
      361 ctcgctgctc gtcaaggtca accagatcgg cacgctcagc gaaacgctcg acgcggtcga
      421 tatggcgcac cgcgcgcgtt ataccgcggt catgtcgcac cgttcgggcg agaccgagga
      481 ttcgacgatc gccgacctcg cggtcgcgac caactgcggc cagatcaaga ccggcagcct
      541 cgcgcgctcg gaccggctcg ccaaatacaa ccagctcatc cgcatcgagg aagaactggg
      601 cgacatggcg cgttaccccg gggcgtcgat tttcggctga tcgcaaagct gcggaaaact
      661 gggcgctgac ggcttgacgc cacgaatcaa ctctgattca agggccggaa tgacgcagcg
      721 ccacaaattc cgcaaatcga tgagccgggc caccggtccg gccattgcgg tgatggtcgt
      781 gctggcgatg atcggataca tcatcttcgg cccgaccggg ctttacgcgt ggggcgaata
      841 tggccagtcg gtcgaaaaga agcgcgtcat tttgagcgag ctgaccaaga agcagaaaga
      901 gcttcagaac cgcgtcaacc tgctcgatcg cggccgggtc gatcccgacc tggccgagga
      961 atatgtccgc gagaagctcg gcgcctatca ccccgacgaa tatatcattc cgatggaacc
     1021 cgacgcgaag ccctgagagc ttaacccctc cactttaggg gaggggcagc gagacttgcc
     1081 agcttgctgg ctagtcgcag cggggcgggg tcatcggccc tgcgcaatgc cgcggacccc
     1141 caccccaacc gctcccctga aggggagggg cttaaatgat ctgcatacgt aggttaggcg
     1201 ccaaccccgc ccatggtggg cgttgccaag tgcggcgcgg ctctcctata gggagccttg
     1261 ccgtaacggc taaatgcaaa ggaaacctcc cttggccaaa gctcccgcgc gcaagtctgc
     1321 cgcgccaaaa aagactgcaa ccaccccggc cgccgcgacc aatcgcgagc agccgcgcga
     1381 ccccgctccc tatgacgcgt cgcccgagga actcgagaaa ttctaccgcg aaatgctgct
     1441 catccgccgc ttcgaagaga aggcggggca gctttatggg ctcggcctga tcggcggatt
     1501 ctgccacctt tacatcggcc aggaagccgt ggcggtcggc ctgcagtcgg cgctcgacgg
     1561 cgacaaggac agcgtcatca ccggctaccg cgaccatggc cacatgctcg cctatggcat
     1621 cgaccccaag gtcatcatgg ccgagctgac gggacgccag gccggcatct cgaagggcaa
     1681 gggcggctcg atgcacatgt tcagcgtcga gcataaattc tatggcggcc acggcatcgt
     1741 cggcgcgcag gtgtcgctcg gcacgggcct tgccttcgcg cacaaatatc gcggcgacgg
     1801 cggcgtggcg atggcctatt tcggcgacgg cgccgcgaac cagggccagg tctatgaaag
     1861 cttcaacatg gccgagctgt ggaagctgcc gatcatcttc gtgatcgaga acaaccagta
     1921 cgccatgggc acctcggtca accgcgcgtc ggccgaggac cagctctatc gccgcggcga
     1981 aagcttccgc attcctggca tgcaggtcga cggcatggac gtgctcgcgg tgcgcggcgc
     2041 ggccgaggcg gcgctcgaat gggtgcgcgc gggcaagggc ccggtgctga tggaactcaa
     2101 gacctatcgc tatcgcggcc actcgatgtc ggaccccgcc aaatatcgca gccgcgagga
     2161 agtgcaggcg gtgcgcgaca agtcggacgc gatcgagcat ctcaagaaac tgatgaccga
     2221 cgccggggtc agcgaagaca agatcaagga tatcgacaag gaaatccgcg cagtcgtcgc
     2281 ggaatcggcc gactttgccg aaagcgcgcc cgaacccgaa ttgtccgaac tttataccga
     2341 cgtgctggtg gagcaatatt gagatggcca tcgaattgaa gatgccggcg ctgtcgccga
     2401 cgatggaaga gggcacgctc gccaagtggc tcgtcaagga aggcgatacg gtgaagccgg
     2461 gcgacatcct tgccgagatc gaaaccgaca aggcgacgat ggaatttgaa gcgatcgacg
     2521 aaggcacgat cgggcagatc ctggtcgccg aaggcaccga caatgtgaag gtcggcaccg
     2581 tgatcgcgac gatcacgggc gagggtgagg aagctgcggc gcctgccgcg gctccggcgc
     2641 c
//
LOCUS       DATMSU010000053        13161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181207,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000053 DATMSU010000000
VERSION     DATMSU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13161
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181207"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VN034_01345"
     CDS             <1..313
                     /locus_tag="VN034_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWV59271.1"
                     /translation="RFAAVAPVAGRGETATACALKDTPIWAFHGDRDDVVVPEGSFAM
                     ARAIRACGGQLSRLTIYPDLGHNSWDPAYDDPALYLWLLSHRLPTPPAAATNKKTKER
                     K"
     gene            310..1656
                     /locus_tag="VN034_01350"
     CDS             310..1656
                     /locus_tag="VN034_01350"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HWV59272.1"
                     /translation="MTKTIFPDDFLWGAATAAYQIEGSPLADGAGASIWQRFSHDPRL
                     MAAKGDTGDVACDHYNRMPADVALMKELGLKAYRFSVNWGRVLPEGIGRINEPGLGFY
                     ERLVDELLKHDIEPLLTLHHWDLPVALDDKGGWLNRDSADWFAEYGSVMYRRLDGRVK
                     KWVTLNEPWVITDGGYLHGALAPGHRNLFETPIASHNLMRAHGRAVQAYRSEGAHEIG
                     LVVNIEPKYPASDSAEDVAATARAHAYMNRQYLDPALKGSYPDELTEIFGEAWPKWSA
                     EDLKDICQPLDFIGINYYTRNVVKADPHQWPVGASPVRQVATHTTTDWEVYPPALTDM
                     LVWFRDTYGDIPVYITENGAAFYDPPKAGPDGIDDPLRCDYLRTHISAIGDAIRQGVD
                     VRGYMAWSLLDNLEWSLGFSKRFGIVHVDYETQVRTPKRSARFYSGVIATNGGNLG"
     gene            1653..2522
                     /locus_tag="VN034_01355"
     CDS             1653..2522
                     /locus_tag="VN034_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWV59273.1"
                     /translation="MIRKAMLLAAAALLALPAAAKERADHYEAMTYNIRLDIASDGDN
                     AWPHRRAALIALVVYQAPDLVGMQEVLQHQKQAVEADLPAYQFVGVARDDGKTKGEYS
                     MLGFRRDRFALVGSGTFWLSPTPDVPSKGWDAALPRIATWARLHDQAAKRDLLVVNTH
                     FDHIGEVARQESARQIRRWIDGERKPGETAVLMGDFNSPATSTAYAAIVEPGKGALHD
                     SRTVSRTPHYGPLGTFTGFDIARMDASPIDHIFVSDDVTVLRHATLTDQNGGKLPSDH
                     YPVVADLCIGKGC"
     gene            complement(2679..4166)
                     /locus_tag="VN034_01360"
     CDS             complement(2679..4166)
                     /locus_tag="VN034_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWV59274.1"
                     /translation="MLFRSGALFLPLIALATACTPVPAPSAATPAPTLAPLTEAAFSE
                     ELTERTFRYFWDTTDTDKCLAPDRWPSNPFSSIAATGFALTAYGIGAERGYVTRAEAA
                     ERTRDCLRFYWTAPQGPEAAGNTGYKGFFYHFLRNDDGTRFQTNELSTVDTSLFLGGA
                     LFAQSYFDRDDPVEAEIRDLAEKIYTRVDWTFVQRTNTGTQARNLANSHGITMGWKPE
                     KGFETHDWVGYNEGMLVYILALGSPTHPVGKDAWDKGWAADLEKDWGTYYGQEHLQFE
                     PLFGHQYSHVWVDFRGIRDAFMRGKGIDYFENSRRATLAQRAYGADNPNKWAGYSADI
                     WGWTASDGPGYSQGKYSVDGTPRDFNGYMARGVSAIRVVDDGTIVPTAAGGSVAFAPE
                     TAIPALMAMRTQYGPRLYTRYGFKDAFNPSFTFTDADSKSGEVDPVHGWVANDYLGID
                     QGPILAMMENHRSELVWKVMRKNPHIMRGLKRIGFTGGWLDQAAE"
     gene            complement(4237..7299)
                     /locus_tag="VN034_01365"
     CDS             complement(4237..7299)
                     /locus_tag="VN034_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59275.1"
                     /translation="MKVSHEFQTMTARRSRARRLAAALAWSSAGAALAMAVATPAHAQ
                     VSGASLRGTVKAEGGVSQVTAINVDTGLTRTSTVGANGSYNFASLPVGTYRLELTTPQ
                     GKRQTDEFTLNVAQNAVLDFDFSQPDIAEGGDDAIIVTGSRLRSMEGGEVGTTISQRL
                     IEQLPQNNRNFLAFADLAPGVQFITNASGQSRLQGGAQGSNSVNIFIDGVGQKDYVLK
                     NGITGQDSTQGNPFPQLAIGEYRVISSNYKAEYDQVSSVAITAVTKSGTNEFHGEGFI
                     DFTNQSLRDRTPAENSPTYIPKIKTRDIQFGGALGGPIIKDVLHFFATYEGKRRTEPR
                     TIRPGLNLSPDFFPSEYQSYFGSVSETFNENLYFGKINFTPTSSDLFEFSAKYRDETG
                     VQINNGIDAFDTRTLAKVEEWRGLARWEHTADTWVNDFKVAYEDVTWGPRPAVFGNVS
                     LFNATVPGAEPGTTQRGDILRIGAGRNFQDKGQTGWQVSNDFTYTGLEGHSFKVGVKA
                     KWVKLNTNEQGGVNPLFTYNALYPAGVDFNDSIPYRMEFSAPVTDGDPNIRSNNFQFG
                     VYAQDDWEVTDRLTVNIGLRWDYERTPAFLDYVHDPAIAAFVSGQAAYTAPDGTVTPP
                     YTNLLNADYDINDYISTGSERKAFKGAWQPRIGFTYELDEEGRFAVFGGYGRSYDRTQ
                     FDFIQQELRQGLFANRIFNFNNPGDTTNVCDPSPTCIAWDPVYLTPEGRADLIAGLPP
                     GAGRELRFIKNDLKMPYSDQFSLGVRGRFDLLDAEVGYSYVTSKDGFVYLLGNRRPDG
                     SFFPPTGNPDSPFGSPPAPFGSIILGDNGLETKAHTAYLKLTKRYTQASPWSLDATYT
                     YTDAKENRQFGEVFSLDFPGIDDYDFITATGVRKHRLVMAGTVDIPWGFTLSGKFQIA
                     SPKYLGAILNTEGTGGTPPSREAISTKTEGNGDRWGFRQMDLSITKYVPIGFINDEAR
                     LRFRLDIINLFNDRNFGGFDALTGLRNPNDLALDGPPRTIKLSAGFSF"
     gene            7551..9869
                     /gene="bglX"
                     /locus_tag="VN034_01370"
     CDS             7551..9869
                     /gene="bglX"
                     /locus_tag="VN034_01370"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HWV59276.1"
                     /translation="MPPMTRNLTCLTLATLMAAGQLSPTPLMAQPAPSKSEAAPEDST
                     GWMRPDPKMDRFIAELMAKMTLDEKIGQLSLLTSDWDSTGPTMRKGYQDDIRKGRIGS
                     IFNAFTAKYTRDLQRLAVEETRLKIPLLFGYDVIHGHRTIFPISLGESASWDLKAIEK
                     AARIAATEASAEGIHWTFAPMVDVARDPRWGRMSEGAGEDVYLGSKIAAARVRGFQGD
                     DLKRVDTVLATAKHFAAYGAAQAGRDYHTVDISERTLRDVYLPPFKAAADAGAATFMT
                     SFNEYDGVPASGSHYLLTDLLREKWGFKGFIVTDYTSINEMVPHGYSKDLKQAGEQAI
                     NAGVDMDLQGAVFMDHLAQSVAEGKVDVKRIDAAVKAILEMKYRLGLFEDPYRYSDEA
                     REKATIYRPDFLEAARDVARKSIVLLKNKDNALPLAAAAKSIAVIGPLGNSKEDMIGS
                     WSAAGDRKTRPVTLLEGMQAGAPKGTSVAFAKGASYAFADAGKTDGFAEAIALAQKSD
                     VIIAAMGERWDMTGEAASRTSLDLPGNQQALLQELKKTGKPIILVLMSGRPNSIEWAD
                     ANVDAILEAWYPGTMGGHAIADVLYGRYNPSGKLPVTFPRNVGQVPIHYDMKNTGRPI
                     NPADPNAKYVSRYLNTPNTPLYPFGYGLSYTSFSYSPVTLDRAKIRPGETLTASVTVT
                     NSGARDGEEVVQLYVRDLVGSVTRPVKELKGFEKISLKKGEKRTVRFTLTDADLAFTR
                     QDMSWGSEPGQFKLWVGPSSAEGQEASFELTE"
     gene            9871..11430
                     /locus_tag="VN034_01375"
     CDS             9871..11430
                     /locus_tag="VN034_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWV59277.1"
                     /translation="MRFRLPVALALPLAFVAAAGAQPAARPNIVYIMSDDHAYQAISA
                     YGSPLSKLAPTPNIDRIAKNGAIFTQSFVGNSLCGPSRATLLTGRQSHAHGFTQNGQR
                     FDNRVWVWPRALSQAGYATAMFGKWHINRSPEGIGFDDWKILDDQGEYYNPDIITPKG
                     RSVVEGYATDLITQYSLDWLKNGRDKTKPFAILIHHKAPHRNFMPALRHVRKYQGVTF
                     PVPGNYFDDYRGRIAAGTQEMNIYRDMYEGHDLKMTVAKGSAQLRYNRWPGAFDRMTH
                     AQQAEWDAAMQADNDRLNATPLSGRDLALWKYQRYMQQYLGTIAAVDEGVGAVLDYLE
                     ESGLDRNTIVVYTSDQGFYLGEHGWFDKRFIYEESMRTPFLIQYPGHIRPGTRIAAPI
                     QNIDYAPTFLDYAGVKGPATIQGRSMTPLLAGRTPRDWRKDVYYHYYEFPGFHSVRAH
                     YGVRGERYKLVRFYGEGLDAWEFYDLESDPHEMNNRIDDPAMKAPIAAMKRRLVELRR
                     EYQDDSGPAIR"
     gene            11471..12700
                     /locus_tag="VN034_01380"
     CDS             11471..12700
                     /locus_tag="VN034_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59278.1"
                     /translation="MNSEPPSSFAPFRYPAFRAIWIANLASNMGSMIQSVAAAWLMTE
                     LTESHLLIALVAASTTIPVMLLGIFAGAIADNFDRRRVMLAAQTGMLIVSAALTVTTY
                     MDAITPLTLLFFTLAVGCGTALNGPAWQASVRLQVGPRDLPQAIALNTIAFNLARSVG
                     PALGGMLVAIVGTAAAFGLNALSYVALIVVLLRWHPETQPPRRTPMLTAIAAGIRFCA
                     HSDPVRRVLIRGFAFGVGAAGFQALLPSLVRDRLHGTEIVYGLCLAAFGAGSIFAALW
                     VGAARRRWGSDRVVTTASLIFAAAMLLVALAANLPALMIAAFVAGGAWVSTLTTLNVA
                     MQLRSPEEILGRCLSIYQAVTFGAMALGAYTLGLVADLSNLPAAILGSAGWLLLSALV
                     LRFVAPMPRRDEGRILP"
     gene            complement(12704..>13161)
                     /locus_tag="VN034_01385"
     CDS             complement(12704..>13161)
                     /locus_tag="VN034_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV59279.1"
                     /translation="NGEVGARRPGGRPLVDTMIELFGDRWATLVVRAMFTHINRFDDI
                     QRDTLMATNILTGRLERLVRQGILKTVPYSSHADRVEYRLTDKGRDLYPVLLALLQWG
                     DKWFSDERGAPVLLTHRPCGHDLHMVAACSHCGDELALSNSRFTIEEAD"
BASE COUNT         2382 a         4118 c         4280 g         2381 t
ORIGIN      
        1 ccgcttcgcg gcggtcgcac cggtcgcggg gcgcggcgag acggcgaccg cttgcgcact
       61 caaagacacg ccgatctggg cctttcacgg cgaccgcgac gatgtcgtcg tacccgaggg
      121 cagcttcgcg atggcgcgcg cgatccgcgc ctgtggcgga cagctctcgc ggctgaccat
      181 ctaccccgac ctcgggcata acagctggga cccggcctat gacgatcccg cgctttatct
      241 ctggctgctc tctcaccgcc tccccactcc gcccgccgcg gcaacaaaca agaaaaccaa
      301 ggaaagaaaa tgacgaagac gattttcccc gacgatttcc tgtggggcgc ggcaaccgcg
      361 gcctatcaga tcgaggggtc accgctcgcc gacggcgcgg gcgcgagcat ctggcagcgc
      421 ttcagccacg acccacggct gatggcggcg aaaggcgaca ccggcgacgt cgcgtgcgat
      481 cattataacc gcatgcccgc cgacgtcgcg ctgatgaagg aattggggct caaggcctat
      541 cgcttcagcg tcaactgggg ccgcgtgctg cccgagggca tcgggcgcat caacgaaccc
      601 gggctcggct tttacgagcg gctggtcgat gaattgctga agcacgacat cgaaccgctg
      661 ctgacgctcc accattggga tctgcccgtc gcgctcgacg acaagggcgg ctggctgaac
      721 cgcgacagcg ccgactggtt cgccgagtac ggttcggtga tgtaccgccg cctcgacggg
      781 cgggtgaaga aatgggtgac gctgaatgag ccttgggtga tcaccgacgg cggctatctc
      841 catggcgcgc tcgcgcccgg ccatcgcaac ctgttcgaga cgccgatcgc gagccacaat
      901 ctgatgcgcg cgcacggccg cgcggtgcag gcctatcgca gcgaaggcgc gcacgagatc
      961 ggcctcgtcg tcaatatcga gcccaaatat cccgcgagcg acagcgcgga ggatgtcgcc
     1021 gcgaccgcgc gcgcgcacgc ctatatgaac cgccaatatc tcgacccggc gctaaaaggt
     1081 tcctaccccg acgaacttac cgaaatcttt ggcgaggctt ggcccaaatg gtccgccgag
     1141 gatcttaagg acatctgcca gccgctcgat ttcatcggga tcaattatta cacgcgcaac
     1201 gtcgtgaagg ccgacccgca tcaatggccg gtcggcgcat cgccggtgcg gcaggtcgcg
     1261 acgcacacga cgaccgactg ggaagtctat ccgcccgcgc tcaccgacat gctggtctgg
     1321 ttccgcgaca cctatggcga tattcccgtc tatatcaccg aaaacggcgc cgccttttac
     1381 gatccgccca aggccggtcc cgacggcatc gacgatccgc tgcgctgcga ttatctgcgc
     1441 acgcatatct cggcgatcgg cgatgcgatc cggcaggggg tcgacgtgcg cggctatatg
     1501 gcctggtcgc tgctcgacaa tctcgaatgg tccttgggct tttcgaagcg gttcggtatc
     1561 gtccatgtcg actatgaaac gcaggtccgc actccgaagc gcagcgcgcg tttctatagc
     1621 ggcgtgatcg cgaccaacgg aggaaatctc ggatgatccg caaagccatg ctgctcgccg
     1681 cggcggcgct gctcgcgctg cctgccgccg ccaaagagcg cgccgaccat tatgaagcga
     1741 tgacctataa tatccggctc gatatcgcgt cggacggcga taatgcgtgg ccgcaccgcc
     1801 gcgcggcgct gatcgcgctc gtcgtctatc aagcgcccga cctcgtcggg atgcaggagg
     1861 tgctgcagca ccagaagcag gcggtcgagg ccgacctgcc cgcctatcaa ttcgtcgggg
     1921 tcgcgcgcga cgacggcaag acgaagggcg aatattcgat gctggggttc cgccgcgacc
     1981 gcttcgcgct ggtcggctcg ggcaccttct ggctctcgcc gacccccgat gtgccgagca
     2041 agggctggga cgccgcgctg ccgcggatcg cgacctgggc gcggctgcac gatcaggcgg
     2101 cgaagcgcga tttgctcgtc gtgaacaccc atttcgacca tatcggcgag gtcgcccggc
     2161 aggaaagcgc ccggcagatc cgccgctgga tcgacggcga acgcaagccg ggcgagaccg
     2221 cggtgctgat gggcgatttc aacagcccgg cgacgagcac cgcctatgcc gcgatcgtcg
     2281 agccgggtaa gggcgcgctg cacgacagcc gcacggtcag ccgcacgccg cattacggcc
     2341 cgctcggcac cttcaccggc ttcgacatcg cgcggatgga cgcgagcccg atcgaccata
     2401 tcttcgtcag cgacgatgtg acggtgctgc gccatgcgac actgaccgac cagaatggcg
     2461 gcaagctgcc gtcggatcat tatccggtgg tcgcggatct gtgtatcggg aagggctgct
     2521 gacggcaggg gccaaaatct gatccgcatc cccgagcgaa gacgaggggc tttgccgagc
     2581 gaagtcgagg ctgcgacgtc cgaggttctc gcttcgctcg aacgggcccc tcgtcttcgc
     2641 tcggggatgc ggatgattca gattttgcgc aattagggct attccgccgc ttggtccagc
     2701 cagccgccgg taaagccgat gcgcttcaat ccgcgcatga tgtgcgggtt tttgcgcatc
     2761 accttccaca ccagttcgct gcgatggttt tccatcatcg cgaggatcgg cccctggtcg
     2821 atgccgagat aatcgttcgc gacccagccg tggacgggat cgacctcgcc cgatttcgag
     2881 tccgcgtcgg tgaaggtgaa gctcgggtta aacgcatcct tgaagccgta gcgcgtatag
     2941 agccggggac cgtattgcgt ccgcatcgcc atcagcgcgg ggatcgccgt ttcgggcgcg
     3001 aaggcgaccg agccgcccgc cgcggtcggg acgatcgtgc cgtcgtcgac gacacggatc
     3061 gcgctgacgc cgcgcgccat atatccgttg aaatcgcgcg gcgtgccatc gacgctatat
     3121 ttgccttgcg aatagccggg tccgtcggag gcggtccagc cccagatgtc ggcgctgtaa
     3181 cccgcccatt tgttcggatt gtcggcacca taagcgcgct gcgccagcgt cgcgcggcgg
     3241 ctgttctcga aataatcgat ccccttgccg cgcatgaaag cgtcgcggat gccgcggaaa
     3301 tcgacccaga cgtggctgta ctgatgcccg aacaacggtt cgaactggag atgctcctgc
     3361 ccgtaatagg tgccccaatc cttttcgagg tcggcggccc agcccttgtc ccacgcatcc
     3421 ttgccgacgg ggtgcgtcgg cgaaccgagc gcgaggatat agacgagcat cccctcgtta
     3481 tagccgaccc aatcgtgcgt ctcgaacccc ttttcgggtt tccagcccat cgtgatgccg
     3541 tgcgagttcg cgaggttgcg cgcctgcgtc ccggtgttcg tccgctgcac gaaggtccag
     3601 tcgacgcgcg tgtagatctt ctccgcaaga tcacggattt ccgcctcgac cgggtcgtcg
     3661 cggtcgaaat agctttgcgc gaacagcgcg ccgccgagga acaggctggt gtcgacggtc
     3721 gaaagctcgt tggtctggaa gcgcgtgccg tcgtcgttcc gcaggaaatg atagaaaaag
     3781 cccttgtagc cggtgttgcc cgcggcctcc ggcccctgcg gcgcggtcca gtagaagcgc
     3841 aggcaatcgc gcgtgcgttc cgccgcttcg gcgcgcgtca cgtaaccgcg ctcggcgccg
     3901 atgccatagg cggtgagcgc aaagccggtc gccgcgatcg acgagaaggg attgctcggc
     3961 cagcgatcgg gcgcgaggca tttgtcggta tcggtggtgt cccagaaata gcggaaggtg
     4021 cgttcggtga gttcttccga aaaggccgcc tcggtcagcg gcgccagcgt cggtgcgggg
     4081 gtggcggccg agggcgccgg aacaggtgtg caagcggtgg ccagcgcgat caggggcagg
     4141 aaaagggccc cgctgcgaaa caacattcga tactccaaaa aagagaggcg cccggcccgc
     4201 gggagggggt tgcgggccga gcgcctggtc agccggtcag aaagagaaac cggccgaaag
     4261 cttgatcgtt cgcggcggcc cgtcaagagc caggtcgttg ggattgcgca gaccggtgag
     4321 cgcgtcgaag ccgccgaaat tgcggtcgtt gaacaggttg atgatgtcga ggcggaagcg
     4381 cagccgcgct tcgtcgttga tgaaaccgat cggcacatat ttggtgatcg aaaggtccat
     4441 ctggcggaaa ccccaccggt cgccattgcc ttcggtcttg gtcgagatcg cttcgcgcga
     4501 cggcggcgtg ccgccggtgc cctcggtgtt caggatcgct cccagatatt tcggcgaggc
     4561 gatctggaac ttgcccgaca gggtgaatcc ccacggaata tcgacggtgc ccgccatcac
     4621 caggcgatgc ttgcgaacgc ccgttgcggt gatgaaatca tagtcgtcga tacccgggaa
     4681 atcgaggctg aagacttcgc cgaactgccg gttttccttg gcatcggtat aggtataggt
     4741 cgcatcgagg ctccagggcg aggcctgggt atagcgcttg gtcagcttga gataggcggt
     4801 gtgggccttg gtttcgaggc cattgtcgcc gagaatgatc gatccgaacg gcgccggtgg
     4861 cgaaccaaag ggcgaatcgg gattgcccgt cggcgggaag aaactgccgt caggccgccg
     4921 gttgcccagc agatagacga agccgtcctt gctcgtgacg tagctgtagc cgacttcggc
     4981 atcgagcagg tcgaagcgcc cgcggacgcc gaggctgaac tggtccgaat agggcatctt
     5041 gaggtcgttc ttgatgaagc gcagttcgcg cccggcgccc ggcggcagtc ccgcgatcag
     5101 gtcggcgcgt ccctcggggg tcagatagac ggggtcccag gcgatgcagg tggggctggg
     5161 gtcgcagaca ttggtcgtat cgcccggatt gttgaaattg aagatgcggt tggcgaacag
     5221 cccctgccgc agttcctgct ggataaaatc gaactgcgtc cggtcataag aacggccata
     5281 tccgccgaag acggcgaagc gaccttcctc gtcgagctcg taggtaaagc cgatacgcgg
     5341 ctgccacgcg cccttgaacg cctttcgctc gctgccggtc gagatatagt cgttgatgtc
     5401 gtaatcggcg ttgagcaggt tcgtataggg cggcgtcacc gttccgtcgg gggcggtata
     5461 ggccgcctgc ccgctgacga acgccgcgat cgccgggtcg tggacatagt cgaggaaagc
     5521 cggcgtccgc tcataatccc agcgcaggcc gatgttgacc gtcaggcggt cggtgacttc
     5581 ccaatcatcc tgcgcataga cgccgaactg gaaattgttc gagcggatat tgggatcgcc
     5641 gtcggtcacc ggcgcactga attccatccg gtagggaatg ctgtcgttga aatcgacgcc
     5701 agcgggatag agcgcattgt aggtgaagag cggattgacc ccgccctgtt cgttggtgtt
     5761 cagcttgacc cacttcgcct tcacgccgac cttgaagctg tggccttcaa ggcccgtgta
     5821 ggtgaaatcg ttcgaaacct gccagccggt ctgtcccttg tcctgaaaat tgcgtcccgc
     5881 gccgatgcgc aggatatcgc cgcgctgcgt cgttcccggc tccgcgccgg gaacggtcgc
     5941 gttgaacagg gagacgttgc cgaacacggc cgggcgcggc ccccaggtca catcttcata
     6001 ggcgaccttg aagtcgttga cccaggtgtc cgccgtatgc tcccagcggg cgaggccgcg
     6061 ccattcctcg accttcgcga gcgttcgcgt gtcgaacgca tcgatcccgt tgttgatctg
     6121 cacgccggtt tcgtcgcgat attttgcgga aaattcgaac agatcgctcg atgtcggggt
     6181 aaagttgatt ttcccgaaat agagattctc gttgaacgtc tcgctcaccg agccgaagta
     6241 ggattgatat tcggacggga agaaatcggg cgaaaggttg agacccggcc tgatcgtgcg
     6301 aggctcggtg cggcgcttgc cttcataggt cgcgaagaag tgcagcacgt ccttgatgat
     6361 cggcccgccg agcgcgcccc cgaactggat gtctcgggtc ttgatcttgg ggatataggt
     6421 cggggaattt tccgccgggg tgcggtcgcg caggctttgg ttggtgaaat cgatgaagcc
     6481 ctcgccgtgg aattcattgg ttcccgattt ggtcaccgcg gtgatcgcga ccgaactgac
     6541 ctggtcatat tcggccttat aattcgagct gatgacccgg tattcgccga tcgcgagctg
     6601 agggaacggg ttgccttgcg ttgaatcctg gccggtgatg ccgttcttga ggacatagtc
     6661 cttttgcccg acgccatcga tgaagatatt gaccgaattg ctgccctgcg cgccaccctg
     6721 caaacgcgac tggccgctcg cattggtgat gaactgcacg cccggcgcaa gatccgcaaa
     6781 ggcgaggaaa ttgcggttgt tctgaggtag ctgctcgatc aggcgctgcg aaatggtcgt
     6841 cccaacctcg ccgccctcca tcgaacggag gcggctgccg gtgacgatga tcgcatcgtc
     6901 accgccctcg gcgatgtcgg gctgcgagaa atcgaagtcg agcacggcat tctgcgcgac
     6961 gttgagcgtg aattcgtcgg tctggcgctt gccctgcggt gtcgtcagtt cgaggcgata
     7021 agtgccgacg ggcagggagg cgaaattgta gctgccgttc gcgccgacgg tcgaggtgcg
     7081 cgtcaggccg gtgtcgacgt tgatcgcggt aacctgggac acgccgccct ctgccttcac
     7141 cgtaccgcgc agcgaagcgc cggagacctg ggcgtgcgcg ggcgttgcga ccgccatcgc
     7201 gagcgcggcg cccgcgctgc tccatgcaag cgccgcggcg agccggcgtg cgcgcgaccg
     7261 gcgtgcggtc atggtctgaa attcatgcga aaccttcacg gcaacctccc tttttgtgcc
     7321 ccgacatttc ccgccctcgc tctattcgcc gaccggcctt gcctccgggt acagcgacga
     7381 gtcgcgggaa atcgcgtcgt tgtttttttg atttgtaacc ggtttcacga tagctgtata
     7441 gggggaagcg acacagatgg atgcgatcgg gggccagccg gtttttgggc cgaaattcgg
     7501 ccaatccgcg tgacaaatgt ataaacagac ccgaccggga aggacgaatc atgccgccga
     7561 tgacgcgcaa tctcacatgc ttgacgctcg ccaccttgat ggccgcaggg cagctatccc
     7621 ccacgccgct gatggcgcaa ccggcaccga gcaagagcga ggccgctccg gaggattcca
     7681 cgggatggat gcgccccgat ccgaagatgg accgctttat cgccgagctg atggcgaaga
     7741 tgacgctcga cgaaaagatc ggtcagctgt cgctgctcac cagcgactgg gattcgactg
     7801 ggccgacgat gcggaagggc tatcaggacg atattcgcaa ggggcggatc gggtcgatct
     7861 tcaacgcctt taccgccaaa tatacgcgcg acctgcaacg gctcgcggtc gaggaaacgc
     7921 ggctcaagat tcccctgctc ttcggctatg acgtcatcca cggccaccgc acgatattcc
     7981 ccatctcgct cggcgaatca gcaagctggg atttgaaagc cattgaaaag gcggcgcgaa
     8041 tcgcggcgac cgaagcgtcg gccgagggca tccactggac cttcgcgccg atggtcgacg
     8101 tcgcgcgcga tccgcgctgg ggccgcatgt ccgaaggcgc gggcgaagat gtgtatctgg
     8161 gcagcaagat cgccgcggca cgcgtgcgcg gtttccaggg cgacgatctg aagcgcgtcg
     8221 ataccgtgct ggcgacggcg aagcattttg ccgcttatgg cgccgcgcag gcgggccgcg
     8281 actatcatac ggtcgatatt tcggaacgca cgctgcgcga cgtctatctg ccgccgttca
     8341 aggccgccgc cgatgcgggg gccgcgacct tcatgacgtc gttcaacgaa tatgacggcg
     8401 tgccggcatc gggcagccat tatctgctga ccgaccttct gcgcgagaaa tgggggttca
     8461 agggtttcat cgtaaccgat tacacgtcga tcaacgaaat ggtcccgcac ggctattcga
     8521 aagacctcaa gcaggcgggc gagcaggcga tcaacgccgg ggtcgacatg gaccttcagg
     8581 gcgcggtgtt catggatcac ctcgcgcagt cggtcgccga gggcaaggtc gacgtgaagc
     8641 gcatcgatgc cgcggtaaag gcgatcctcg aaatgaagta tcgcctcggc ctgttcgagg
     8701 acccctatcg ctattcggat gaagcgcgcg aaaaggcgac catctatcgc cccgacttcc
     8761 tcgaagccgc gcgtgatgtc gcgcgcaagt cgatcgtcct gctcaagaac aaggacaatg
     8821 cccttcccct cgccgcggcg gcgaagtcga tcgcggtgat cggcccgctc ggcaacagca
     8881 aggaggatat gatcggcagc tggtccgcgg cgggcgaccg caagacgcgg ccggtcaccc
     8941 tgctcgaagg gatgcaggcc ggcgcgccca aggggacgag cgtcgctttt gccaagggcg
     9001 cgagctatgc gttcgccgac gcgggcaaga ccgacgggtt cgccgaagcg atcgcgctgg
     9061 cgcagaaatc ggacgtgatc atcgcggcga tgggggaacg ctgggacatg acgggcgagg
     9121 cggcgagccg cacgtcgctc gacctgcccg gcaaccagca ggcgctgctt caagaattaa
     9181 agaagaccgg caagccgatc atcctcgtgc tgatgagcgg gcgaccgaac agcatcgaat
     9241 gggccgacgc aaatgtcgat gcgatcctcg aagcctggta tccgggcacg atgggcggcc
     9301 acgcgatcgc cgatgtcctc tatggccggt acaatccgtc gggcaagctg cccgtcacct
     9361 ttccgcgcaa cgtcgggcag gtgccgatcc attacgacat gaagaacacc ggtcggccga
     9421 tcaatccggc ggatcccaac gcgaaatatg tatcgcgcta tctcaatacc ccgaacacgc
     9481 cgctctatcc gttcggctat ggcctcagct atacaagctt ttcctattcg ccggtgacgc
     9541 tggacagagc gaagattcgc cccggcgaga cactgacggc gagcgtcacc gtgacgaaca
     9601 gcggcgcacg cgacggcgag gaggtcgtgc agctctatgt ccgcgatctt gtcggatcgg
     9661 tgacgcggcc ggtcaaggag ttgaaggggt tcgagaagat cagcctcaag aagggcgaaa
     9721 agcggacggt ccgcttcacg ctgaccgacg ccgatctggc cttcacgcgg caggatatga
     9781 gctggggcag cgagcccggc cagttcaagc tgtgggtcgg gccgtcgtcc gccgagggtc
     9841 aggaagccag cttcgagctg accgaatagg atgcgctttc gccttccggt cgcgctcgca
     9901 ctcccgctcg ctttcgttgc cgcggcggga gcgcagccgg ccgcgcgccc gaacatcgtc
     9961 tacatcatgt cggacgacca cgcctatcag gcgatttcgg cctatggctc gccgctgtcg
    10021 aagctggcgc cgacgcccaa tatcgaccgc atcgcgaaaa atggcgcgat cttcacgcaa
    10081 agcttcgtcg gcaattctct ctgcggcccc agccgcgcga cgctgctcac cgggcggcag
    10141 agtcacgcgc atggctttac ccaaaacggc cagcggttcg acaacagggt ctgggtctgg
    10201 ccgcgcgcgc tgagccaggc gggctatgcg accgcgatgt tcggcaagtg gcacatcaac
    10261 cggtcgcccg aggggatcgg cttcgacgac tggaagatac tcgacgacca gggcgaatat
    10321 tataatcccg acatcatcac gccgaagggg cgcagcgtcg tcgaaggcta tgccaccgac
    10381 ctcatcaccc agtacagcct cgactggctg aaaaacgggc gcgacaagac gaagccgttc
    10441 gcgatcctga ttcaccacaa ggcgccgcac cgcaacttca tgcccgcgct gcgccatgtg
    10501 cggaaatatc agggcgtgac ctttcccgtg ccgggcaatt atttcgacga ttatcgcgga
    10561 cgcatcgccg ccggcacgca ggaaatgaac atctatcgcg acatgtacga ggggcacgac
    10621 ctcaagatga cggtcgcgaa gggatcggcg caactgcgct ataatcgctg gcccggcgcg
    10681 ttcgaccgca tgacccacgc gcagcaggcc gaatgggacg cggctatgca ggccgacaac
    10741 gaccggctca acgccacgcc gctgtcgggc cgcgacctcg cgctgtggaa atatcagcgt
    10801 tatatgcagc aatatctggg gacaatcgcc gcggtcgacg aaggcgtcgg cgcggtgctc
    10861 gactatctgg aggaaagcgg cctcgatcgg aacacgatcg tcgtctacac ctccgatcag
    10921 ggcttttatc ttggcgagca tggctggttc gacaaacgct tcatctatga agaatcgatg
    10981 cgcaccccgt ttctgatcca gtatccgggg catatccgcc ccggcacgcg catcgcggcg
    11041 ccgatccaga atatcgacta tgcgccgacc ttcctcgact atgccggggt caagggcccg
    11101 gcgacgattc agggccgctc gatgacgccg ctgctcgccg gccgcacgcc gagggactgg
    11161 cgcaaggacg tttattatca ttattatgaa tttcccggat tccactcggt acgcgcgcat
    11221 tacggcgtgc gcggcgagcg gtacaaactc gtgcgcttct atggcgaggg gctcgacgcg
    11281 tgggaatttt acgatctcga gagtgatccg cacgagatga acaatcggat cgacgacccg
    11341 gcgatgaaag cgccgatcgc ggcgatgaag cggcggctcg tcgagctgcg ccgcgaatat
    11401 caggatgata gcggtccagc catccgctga gcggcttgcg gtggttgctg gcgggcggta
    11461 gcgaaacgga gtgaactccg aaccgccatc gtctttcgca cccttccgct acccggcgtt
    11521 ccgcgcgatc tggatcgcca acctggcgtc gaacatgggg tcgatgatcc agtcggtcgc
    11581 ggcggcgtgg ctgatgaccg agctgaccga atcgcatctg ctgatcgcgc tggtggcggc
    11641 gagcacgacg atcccggtca tgctgctcgg catcttcgcg ggcgcgatcg ccgacaattt
    11701 cgaccggcga cgcgtgatgc tcgcggcgca gacggggatg ctgatcgtct cggcggcgct
    11761 gacggtcacg acctatatgg atgcgatcac gccgctgacg ctgcttttct ttacgctcgc
    11821 ggtcgggtgc gggaccgcgc tcaacggccc ggcgtggcag gcgtcggtgc gattgcaggt
    11881 cgggcccagg gatttgccgc aggcgatcgc gctcaacacc atcgcgttca acctcgctcg
    11941 cagcgtcggg cccgcgctcg gcgggatgct ggtcgcgatc gtcggaaccg ccgccgcttt
    12001 cgggctcaac gcgctaagct atgtcgcgct gatcgtcgtg ctgctgcgct ggcatcccga
    12061 aacgcagccg ccgcgaagga cgccgatgct gacggcgatc gccgcgggca tccgcttctg
    12121 cgcgcattcg gacccggtgc gccgcgtgct gatccgcggt ttcgccttcg gtgtcggcgc
    12181 ggcggggttt caggcgctgc tcccctcgct tgtccgcgac cggctgcacg ggaccgaaat
    12241 cgtctatggt ctgtgtcttg cggcttttgg agcggggtcg atctttgccg cgctatgggt
    12301 cggcgccgcg cggcggcgct ggggcagcga ccgggtggtg accacggcgt cgctgatctt
    12361 cgccgccgcg atgctgctcg tcgccctcgc ggccaatctc cccgccttaa tgatcgccgc
    12421 cttcgtcgcg ggcggcgcgt gggtatcgac gctgacgacg ctcaatgtcg cgatgcagct
    12481 gcgatcgccc gaagagatac tcggccgctg cctgtcgatc tatcaggcgg tgaccttcgg
    12541 cgcgatggcg ctcggcgcct atacgctcgg gctggtcgcc gacctcagca acctgcccgc
    12601 ggcgatcctc gggtccgccg gctggctgct actctccgcg ctggttttgc gcttcgtcgc
    12661 gccgatgccg cgccgcgacg agggccgcat cctgccctga acctcagtcg gcctcttcga
    12721 tcgtgaaacg gctgttcgac agcgcaagtt cgtcgccgca atggctgcac gccgccacca
    12781 tgtggaggtc gtggccgcaa gggcgatgcg tcagcagcac cggggccccg cgctcgtcgg
    12841 aaaaccattt gtcgccccat tgcagcagcg cgagcagcac cgggtaaaga tcgcgcccct
    12901 tgtcggtcag gcgatattcg acgcggtcgg cgtggctcga atagggcacg gttttcaaaa
    12961 tgccttgccg cacgagccgt tcgaggcggc cggtcaagat gttcgtcgcc atcagcgtgt
    13021 cgcgctggat atcgtcgaag cggttgatgt gggtgaacat cgcgcgcacg acgagcgtcg
    13081 cccagcggtc gccgaacagc tcgatcatcg tgtcgacgag cggccgcccg ccggggcgcc
    13141 gcgccccgac ttcaccgttg a
//
LOCUS       DATMSU010000054         1826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_95980, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000054 DATMSU010000000
VERSION     DATMSU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95980"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..715)
                     /locus_tag="VN034_01390"
     CDS             complement(95..715)
                     /locus_tag="VN034_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV59280.1"
                     /translation="MEIETATVATPHKGRPREFCVDAALAAALRVFWSKGYEGASMAD
                     LTEAMGITKPSLYAAFGNKEALFNKALDMYEREKADYMRAALEAPTARGVAERLMYGA
                     LDMYTCPENPNGCLGVITSVACGAEAECIRQAVLERGATAKRAMLERFARAKEEGDFP
                     PDTDAEGLAAFLTAVNQGMSVQAGAGATRAELERLVETSLKLWPGR"
     gene            complement(766..1146)
                     /locus_tag="VN034_01395"
     CDS             complement(766..1146)
                     /locus_tag="VN034_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWV59281.1"
                     /translation="MAHGIARIGKDHYRTEIEVGGHALVGDEGPGLGGQNLGPAPYDF
                     LLAGLGACTAITLRMYADRKEWPLESVEVGLKLVNKDGLRIDRVLTIAGLDAEQKARL
                     ADIAERTPVTLTLKGGLAIDTRLA"
     gene            complement(1531..1604)
                     /locus_tag="VN034_01400"
     tRNA            complement(1531..1604)
                     /locus_tag="VN034_01400"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Cys,seq:gca)
BASE COUNT          319 a          586 c          572 g          349 t
ORIGIN      
        1 cgtcaagcgc attctgtacc gggcagtaaa atttttcttt cgaagcgttt actgaaaaag
       61 tgggtgccgg ttcttcagcg gggaaatact cagatcaacg ccccggccac agcttcaggc
      121 tggtttcgac cagccgttcg agttcggcgc gcgtcgcgcc cgcccccgcc tgcaccgaca
      181 tgccctgatt gaccgcggtc agaaaggccg cgagcccttc ggcgtcggtg tcgggcggaa
      241 agtcgccctc ttccttcgcg cgcgcgaagc gttcgagcat cgcgcgcttc gccgtcgcgc
      301 cgcgttcgag caccgcctga cgaatgcatt cggcctcggc gccgcacgcg accgaggtga
      361 tcacgccaag gcagccgttc ggattctcgg ggcaggtgta catgtcgagc gcgccgtaca
      421 tcagccgctc ggcgaccccg cgcgcggtcg gcgcttcgag cgcggcgcgc atatagtcgg
      481 ccttttcgcg ctcatacatg tcgagcgcct tgttaaagag cgcctccttg ttgccgaagg
      541 cggcatagag gctgggcttg gtgatcccca tcgcctcggt cagatcggcc atcgaggcgc
      601 cttcatagcc cttcgaccaa aagacgcgca gcgccgccgc cagcgccgcg tcgacgcaaa
      661 attcgcgcgg gcggcccttg tgcggtgtgg cgacggtggc ggtttcgatt tccataacgg
      721 ctggtatata agtgaggtgg gacaaaatgt ccagtggcgc ggcggtcagg cgagtcgggt
      781 gtcgatcgcc agcccgccct tcagcgtcag cgtcaccggc gtccgctcag caatgtcggc
      841 aagccgcgcc ttttgttcgg cgtcgagccc ggcgatcgtc agcacgcgat cgatccgcag
      901 cccgtccttg ttgacgagct tcagccccac ctcgaccgat tcgagcggcc actccttgcg
      961 gtcggcgtac atgcgcagcg tgatcgcggt gcaggcgccg agcccggcaa gcaggaaatc
     1021 atagggcgcg ggcccgagat tttgcccacc cagccccggc ccctcgtcgc cgaccagcgc
     1081 gtggccgccg acctcgatct cggtgcgata atgatccttg ccgatacggg cgattccatg
     1141 agccatcaga ttgtccttcc gtctagttcc ggaagctatt ttcccgccgc gagagacaga
     1201 cggcaaggcc cggctttcgc cgtctcttga atcgatcacg gaaagcccta gattgaggcc
     1261 atcgtcaaca agcgaaagga ggtggtcgaa tgtctcattg tcacacaccg catgcggcag
     1321 atttgagcat gaccgtcctc gcgggggtca agctcggcta agctgtccgc gacaggcaag
     1381 acaatggaag ggctgtcccg aaaggggcgg ccctttgtcg ttgcggccac cgttccgaaa
     1441 ttgtgacgaa tgtcgcccgc cgtcagcttg ccgtcagcaa agaccgctat cccctgtgcg
     1501 ggggatggca tgatgtcttg gggaaaaatt tggaggcccg agccggaatc gaaccggcgt
     1561 gcaaggattt gcagtcctct gcgtaaccac tccgccatcg ggcctcagac gcagatttgc
     1621 gtggagacag cgcccctagc gatttcgctt cgcactggca atccgatagt tcgcaattcg
     1681 ggcttaaatc ggctcgcgcg ccgcgatgcg ggcttggcgg tccggcgcgg gcgggctatg
     1741 cgcgccgcag cgggccgctt ggctgtatta ctattgcaat acagcatcgc ttcggctaga
     1801 gctgccccgc aaaggcttaa cccagg
//
LOCUS       DATMSU010000055         3739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181233,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000055 DATMSU010000000
VERSION     DATMSU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181233"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VN034_01405"
     CDS             complement(<1..218)
                     /locus_tag="VN034_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59282.1"
                     /translation="MIEQLGRTRDDAYSWMKFIPASGERDRTNLPAPVAKMLADENAY
                     ADAVLKPTEGEQAELVRAMLARGSDAAAA"
     gene            627..2819
                     /locus_tag="VN034_01410"
     CDS             627..2819
                     /locus_tag="VN034_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59283.1"
                     /translation="MGFASIRRIAACGISVLALGYATGALAQEVDDSEIVVTAAKREQ
                     SVRDVSGSVSAVSEATLQKLNAQSLSDYITRVPGVIFNDYQPGVSEVVIRGVASSTYH
                     EANQATTGYYLNEVPLIEPGFPLVIPDVDSFDVARVEVLRGPQGTLFGSSSLGGAINY
                     VVNEADPARLDAGFEGNLATTKGAGEASYAAKAMVNLPIVTDKLAVRLVALQRYDAGY
                     LDNTLLGEDGSNDLRVRGLRGSIVFTPTETTRISALSMYQEYDLDDQTYVIFGPPKTF
                     ERATNVAEYQDTSFMMHSLRIEQDLGFATLTAVGSYTEKKADLAFDNSIFNGNDPRTD
                     TPELASSNGKSKTEYGELRLASPDGGRFRWLLGANYTRLRSNNTDGTYIEGIADYIDA
                     NPGEFDGQPGSELAPGDLATRTISSNRVTEKAIFGEASFDIVDALTLTVGGRLFEYRS
                     RPRLQYLPNANLVDPFDFAPAADKDSDFIPKASLTYKPSDDFMVYALYSEGFRIGGVN
                     VYSAAVPGLPLTFESDTTKNYEIGTRFDLIDRTLSVDVTAYHIDWGNIQARLFTPVDF
                     DAYTVNGGGADIDGVEISLNLRPTRHLSFSSNIAYNDARLSTLLPDSFAPGGGYAKGT
                     RLPGASEWTLSNSLDLSFADAPLKPRFGIAHRYLSSAPVDFAGTLEKGDFHLVDLNAS
                     ITVNKRIELGLFAKNLFNQYGILNAPFAFAGSVARPRTIGATVRFSLD"
     gene            complement(3058..>3739)
                     /locus_tag="VN034_01415"
     CDS             complement(3058..>3739)
                     /locus_tag="VN034_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HWV59284.1"
                     /translation="IEASDELMLELQPAELAAAGEMFRKLAPRDTPLAIEARLSGKAL
                     TGFRALEASGRSFGEDGLDDWAVMVLMGQRVAQNAGLSPADGVETGLTEKFQAAGKPI
                     GGFETAREQLTLFETLDPQTQRILLTRAAEEAGDAVKEVGALTAAWGRGDVAALERVI
                     NEDIDSVPAARKAILTDRNRRWCAWVKRRMERPGTVLMAVGAGHLVGSDGVPAMLEAE
                     GMAVTRVQ"
BASE COUNT          637 a         1260 c         1164 g          678 t
ORIGIN      
        1 gccgcggcgg catccgagcc ccgcgcgagc atcgcgcgca ccagctccgc ctgctcgccc
       61 tcggtcggct tcagcaccgc atcggcatag gcattttcgt cggcgagcat cttcgcgacc
      121 ggagcgggca ggttggtgcg atcgcgttcg ccgctcgctg ggatgaattt catccagcta
      181 taggcgtcgt cgcgcgtgcg gcccaattgt tcgatcacat tcgaaatgcg gggcgcttgc
      241 ggcggaacga ccgcgggcca gcgtccggcc ggattcctgg cccaaacagc gcggtttccg
      301 agcacaaggg ccgccatcgc ggccccgctc ataaacgctc tccgaccgct atttccgatg
      361 ctgcgcatgc gaccctcttt cgaccgacgg ggatgttccg tccagtctat ggcggtgctg
      421 atggcttact gcgccgatca ggccgcccac tccatcgatc gggcaaattg gctgcaagtc
      481 ctcacgaaac cgcgctttcc tgcgcttgtc cgctggcccg atccggctgg attatggccc
      541 gaccatcgcc gtcacgccaa tttcgcgtga tagcttgagg atcggatttt cctaccaata
      601 ggcaatatca aaaaagggag gcagaaatgg ggtttgcatc gatccggcgg atcgccgcgt
      661 gcggcatctc ggtcctggcg ctggggtatg cgacgggggc gctggcgcag gaggtcgacg
      721 acagcgagat cgtcgtcacc gccgccaagc gcgaacagtc ggtgcgcgac gtcagcggat
      781 cggtgagcgc ggtcagcgaa gcgacgctgc agaaactcaa cgcccagagc ctttccgatt
      841 atatcacgcg cgtcccgggc gtcatattca acgactatca gccgggcgtg tccgaggtcg
      901 tgatccgcgg cgtcgcctcg tcgacctatc atgaggcgaa ccaggcgacg acgggctatt
      961 atctcaacga agtgccgctg atcgagcccg gcttcccgct cgtcatcccc gacgtcgaca
     1021 gcttcgacgt cgctcgcgtc gaggtgctgc gcgggccgca gggcaccttg ttcggctcct
     1081 cctcgctcgg cggcgcgatc aactatgtgg tgaacgaggc tgatcccgcg cggctcgatg
     1141 cggggttcga ggggaatctc gcgacgacca agggcgcggg cgaagcgagc tatgcggcga
     1201 aggcgatggt caacctgccg atcgtaaccg acaagctggc ggtccgcctt gtcgcgctcc
     1261 agcggtacga cgcgggctat ctcgacaaca cgctgctcgg cgaagacggc agcaacgacc
     1321 ttcgcgtccg gggtctgcgc ggctcgatcg tcttcacccc gaccgagacg acgcgcatct
     1381 cggcgctcag catgtatcag gaatatgatc tcgacgatca gacctatgtg atcttcggcc
     1441 cgcccaagac gttcgagcgc gcgaccaacg tcgccgaata tcaggacacg agcttcatga
     1501 tgcacagcct gcggatcgag caggatctgg gctttgcgac cctcacggca gtcggcagct
     1561 ataccgagaa gaaggccgat ctggcgttcg acaattcgat cttcaacggc aatgatccgc
     1621 gcaccgacac gcccgaactc gcgagcagca acggcaagtc gaagaccgaa tatggcgagc
     1681 tgcgcctcgc gtcgcccgac ggcgggcgct tccgctggct gctcggcgcc aattacacgc
     1741 ggctgcgctc gaacaacacc gacggcacct atatcgaggg tatcgccgac tatatcgacg
     1801 cgaatcctgg cgagttcgac ggccagccgg gcagcgagct tgcccccggc gatcttgcaa
     1861 cgcggacgat cagcagcaac cgggtcaccg aaaaggcgat cttcggcgaa gcgtcgttcg
     1921 acattgtcga tgcgctgacg ctgaccgtcg gcgggcgctt gttcgaatat cgttcgcgtc
     1981 cgcgcctgca gtatcttccc aacgccaatc tggtcgatcc gttcgacttc gcgccggcgg
     2041 cggacaagga ttcggatttc attccgaagg cctcgctgac gtacaagccg tcggacgact
     2101 tcatggtcta tgcgctctat tcggagggct tccgcatcgg cggcgtcaac gtctattcgg
     2161 cggcggttcc cggcctgccg ctgactttcg aaagcgacac gacgaagaat tacgagatcg
     2221 ggacgcgctt cgacctgatc gaccggacgc tgagcgtcga cgtcaccgcc tatcatatcg
     2281 attggggcaa catccaggcg cgcttgttca ccccggtcga cttcgacgcc tatacggtga
     2341 acggcggtgg tgccgatatc gacggcgtcg aaatcagcct caacctgcgc ccgacgcggc
     2401 atctgagctt ctcgtcgaat atcgcctata atgatgcgcg gctgtcgacc ctgctccccg
     2461 acagtttcgc gccgggcggc ggctatgcga agggaacgcg ccttccgggc gcgtccgaat
     2521 ggacgctgtc gaactcgctc gacctgagct tcgccgatgc gccgctgaag ccgcgcttcg
     2581 gcatcgcgca ccgctatctg tcgagcgcgc cggtcgattt cgcggggacg ctggagaagg
     2641 gcgacttcca cctcgtcgac ctcaatgcct cgatcacggt gaacaagcgg atcgagcttg
     2701 gcctgttcgc gaagaatctg ttcaaccaat atggcatatt gaacgcgccc ttcgccttcg
     2761 ccggctcggt cgcgcggccg cgcacgatcg gtgcgacggt gcgtttcagc ctcgattgac
     2821 gacgggacgg cagccccgcc ggaagagcgg ggcggtcatt ttggaagtga ccggtttcgg
     2881 ctggaagggg tcgttatggg gatggctctc tccccttcag gggagagata cgaaggcttg
     2941 gcagcttgct gcctagccgc agtagagagg ggctggacca tgtccccctc tcccaaccct
     3001 ctcccctgaa ggggagaggg ctatacgacc gcttccaccc cgaaagccgc cgcccgatca
     3061 ctgtacccgc gtcaccgcca tcccctcggc ttcgagcatc gccggaacgc cgtcgcttcc
     3121 caccagatgc cctgcgccca ccgccatcag aacggtccct ggccgttcca tccgccgctt
     3181 cacccacgcg caccagcgcc ggttgcggtc ggtgaggatc gccttgcgcg cggcgggcac
     3241 gctgtcgata tcctcgttga tcaccctttc gagcgccgcg acgtcgccgc ggccccacgc
     3301 cgcggtcagc gcgccgacct cctttaccgc atcgcccgct tcctcggcgg cgcgcgtcag
     3361 caggatgcgc tgggtctgcg gatcgagcgt ctcgaacagg gtcagctgtt cgcgcgcggt
     3421 ttcgaagccc ccgatcggct tgcccgcggc ctggaatttt tcggtcagac ccgtttcgac
     3481 gccgtcggcc ggcgacaggc cggcattttg cgcgacgcgt tggcccatca ggaccatcac
     3541 cgcccaatcg tcgagcccgt cctcgccgaa gctgcggcca ctcgcctcga gcgcccgaaa
     3601 gccggtcagc gccttgcccg agaggcgcgc ttcgatcgcg agcggggtgt cacgcggcgc
     3661 gagtttccgg aacatctcgc ccgcggccgc gagttcggcg ggctgcagtt ccagcatcag
     3721 ctcgtcggac gcctcgatc
//
LOCUS       DATMSU010000056         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_171774,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000056 DATMSU010000000
VERSION     DATMSU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171774"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1027)
                     /locus_tag="VN034_01420"
     CDS             complement(227..1027)
                     /locus_tag="VN034_01420"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWV59285.1"
                     /translation="MGIWLTAIILGIVEGLTEFLPVSSTGHLILATELLGYDAARWAI
                     FNIAIQPGAILAIVVLYRKLFWEMFTGFFRRDPVAIAFVRNLLLAFFPAVILGLAFGD
                     YIELLLENAVVVAWALIIGGFAILLVERFAKTTDVGGVANVSARQSMLVGLVQCIAMI
                     PGVSRSGATILGAMSFGVDRKTAAEFSFFLALPTLTGATVLQLFKHRDAITTNDLSLI
                     AVGSLVSFVVAWAVIKAFLAVVTRYGFAPFAWYRIIAGVAALIWLGMR"
     gene            complement(1027..>1624)
                     /locus_tag="VN034_01425"
     CDS             complement(1027..>1624)
                     /locus_tag="VN034_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HWV59286.1"
                     /translation="SAIGADPESASAYGRSKGDGEAAVRAAFADAAILRPSILFGRED
                     HFINRFATMMRMVPVVPVIAPNAKFQPAYVGDVADAVVAALGDGATGKTFELGGPQVL
                     TMGELLRWVADATGRSPLFVDVPDFVASSLASGFGWAPGAPITKDQWLMLQRDNIVAE
                     GAVGLAELGVTPTSLASVTEGWLVQYRRHGRFAAVASR"
BASE COUNT          351 a          505 c          527 g          241 t
ORIGIN      
        1 ttcggcggag cgcttgtccg gataggattg ttcacgctcc acaaactgga gcatgcgatc
       61 ggaaaccatg caatatactc ttctggatag gcgaattgca tgggcaaaat tggcctgtca
      121 cgaccaaatt gcaagttagg acagtattac taacctaaaa ggagggtgat ttttccccaa
      181 aataggttcg cgcaattatc tttcgtcaat tgataccgta tcggccctat ctcatgccga
      241 gccagatcag cgccgcgacg cccgcgatga ttcgatacca ggcgaagggg gcaaagccgt
      301 aacgcgtgac gaccgcgagg aacgccttga tcaccgccca ggcgacgacg aacgagacga
      361 gcgagcccac cgcgatcagg ctgaggtcgt tggtcgtgat cgcgtcgcgg tgcttgaaca
      421 gctggagcac ggtcgcgccg gtcagcgtcg ggagcgcgag aaagaagctg aactcggcgg
      481 cggtcttgcg gtcgaccccg aacgacatcg caccgaggat cgtcgcgccc gagcggctga
      541 cgcccgggat catcgcgatg cactggacca gcccgacgag catcgattgc cgcgccgaca
      601 cattcgcgac gccgccgacg tccgtcgtct tggcgaaacg ctcgacgagc aggatggcaa
      661 agccgccgat gatcagcgcc caggcgacga cgacggcatt ttcgagcagc agctcgatat
      721 aatcgccaaa ggcgaggccg aggatcaccg cggggaaaaa ggcgagcagc aggttacgca
      781 caaaggcgat cgccacggga tcgcgccgga aaaacccggt gaacatctcc cagaacagtt
      841 tccggtacag gacgacgatc gcaagaatcg cccccggctg gatcgcgatg ttgaagatcg
      901 cccagcgcgc ggcgtcgtag ccgagcaatt cggtagcgag gatgaggtgg cccgtcgagg
      961 agacgggcaa aaactcggtc aaaccctcga cgatgccgag gatgattgcg gtcagccaga
     1021 tgcccattat cgcgaagcga ccgcggcgaa acgcccatgg cggcgatatt ggacgagcca
     1081 gccctcggtg accgaggcga gcgaggtcgg cgtgacgccg agctcggcca gccccacggc
     1141 gccctcggca acgatattgt cacgctggag catcagccac tgatccttgg tgatcggcgc
     1201 gccgggcgcc cagccgaagc cgctcgcgag cgacgaggcg acgaaatcgg gcacgtcgac
     1261 gaacaagggc gagcggccgg tcgcatcggc gacccagcgc agcagctcac ccatcgtcag
     1321 cacctgtggc ccgccgagtt cgaaagtctt gcccgtggcg ccatcgccca gcgccgcgac
     1381 caccgcatcg gcgacgtcgc cgacataggc gggctggaat ttcgcattgg gtgcgatgac
     1441 cggaaccacc ggcaccatgc gcatcatcgt ggcgaagcgg ttgatgaaat ggtcctcgcg
     1501 gccgaacagg atcgacgggc gcaggattgc ggcgtcggca aaggccgcgc gaaccgccgc
     1561 ttcgccgtcg cccttgctgc ggccgtacgc cgaggcgctt tcgggatcgg cgccgatcgc
     1621 cgag
//
LOCUS       DATMSU010000057        11379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_105338,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000057 DATMSU010000000
VERSION     DATMSU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11379
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105338"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="VN034_01430"
     CDS             complement(<1..1872)
                     /locus_tag="VN034_01430"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWV59287.1"
                     /translation="MVAAAIALPTSAAPGGQPKIGKTFADSVPGPINRTTAPAGAPNI
                     LVWVIDDMGFGQLGSYGGLVETPNIDRVAQRGLRYTNYHTTPICSASRAALLTGRNSH
                     RVHIGGHSAIAVGFPGQDALVPRSAGTVAENLRQSGYLTYAIGKWDHLPQADASAAGP
                     FTYWPSGQGFDRYYGFLAFDADNFDPTLWADHQPVTLSPRRDYHLSADMAEKAIGWIG
                     ARDATDPHRPFMMYWATGAVHAPHHAPQHWLDHYKGRFDAGWDVAAQQILDRQKKLGL
                     VPADMTLPPLPAGVKHWADLSRDEQRMFARQMEAFAAQLSHADEQFGRILDALEARGE
                     LDNTVVLITADNGASAEGAPQGTFSEHYFANAYFATLEENLRHYDDWGGPSTYPHYAS
                     GWAVAGNTPFRYYKQTAYEGGTRVPLILSWPGGIAARGELRVQYQHVTDITPTILDAA
                     GIAPAATVNGERQMAFDGVSMKYSFAAPAAPTRHATQYYEMYGNRSIYANGWKAVVPH
                     RLDVWDFMNRPVFSDGNWELYNLAADPGEQRNVAAANPRKLAQMKALFDCEAKANNVY
                     PLTNTSEAWGFVRKQLAADMARRNFLWTYRGATARIPEALAPPVPSTSFTIEAKLDLD
                     "
     gene            2015..3163
                     /locus_tag="VN034_01435"
     CDS             2015..3163
                     /locus_tag="VN034_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWV59288.1"
                     /translation="MKIELDAIYRRIVDHLENGTTDMAADSIEVPAAHFTDADHLARE
                     LEVFRRQPLAAATSMEIPEPGSFVTRDILGVPLLVVRQKDGSVGVFRNMCRHRGGKVE
                     QADKGKKPFFVCAYHGWSFKGDGSLRGVPFEEQYGTLDKGCRNLFSVAAEERHGLIWI
                     KLPQAPQDLGVADYLGDADERLAAYDIDQLVVFMDQKIDLDINWKLVMDGAIDVLHPQ
                     FLHAEGVGKLIQTGAAVWLDLGRHGQSYSARKRLARKLKAGEPIDKGFRYLTGNLMIF
                     PNMSVIPTPDHIEHWTVWPHLTDPGKCHVHIRFLTDPAKLTDEVAARINRSWDILRQA
                     ATEEDFPMEEMIQANAAALPTTDFLYGANEVPTQHLHLQMAREFEALA"
     gene            3234..4769
                     /locus_tag="VN034_01440"
     CDS             3234..4769
                     /locus_tag="VN034_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWV59289.1"
                     /translation="MNIAELVRYWARWRPDHTAVIADDVRTSWAEFDARSDAIARGLR
                     AAGVGKGDRVGMHLDNRIEAPLLTVACLKLGAMIVPLNFKLTGSEILPLLEDADCRIV
                     VSQAKHLDRLEMAAAKLPFAIHSLDGSDVPSFASLSIGTGAVPTEAIGLNDAGFLCYT
                     SGTTGRQKGALLTHGSAMYPGLAKNIAEGLTWRDSIMVAVPFVFTGAIVSCFIQFTVN
                     AGGTMVLESDFDIDRYLDVIQRHRVSAATTVPVVWERLMRSPGFERADLSSMISAAAG
                     GAPVSIDLIEAYRAKGISLIQSYGLTEASGLAATMHGDDAIKHIGWAGRPIVGSEMKI
                     GDADGHALANGEVGEIMVRGPHVMREYWRNPEATAATFAGDWLRTGDTGMMNDEGFVK
                     VVDRSKDMIISGGINVYPAEIEKILSANPHITELAVIGVPDSDWGEVPMIVASGVQDQ
                     AAAMSAFECDGAAQLAPFKRPRRVVFIDEPLPRTLSGKVSKPTLRGLFPAVPEQAKAL
                     FGK"
     gene            complement(4822..6447)
                     /locus_tag="VN034_01445"
     CDS             complement(4822..6447)
                     /locus_tag="VN034_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWV59290.1"
                     /translation="MQYRTSSLGAALTLFLTVPAATAQDSRPVAIPGTAAAACSPEAI
                     GWAGEDVTIGSVRWLSDPAPYCRIDGTVRTDDPGPNSVGFMIALPQSWNGRYLMVVPG
                     GSAGYIVNPSREHISAGYAVASTDKGSHSTGALDMSFRADPGKSEDYAHRGAHVAALA
                     TQAIARGYYDRQRMPRYIMGCSGGGVSTLMEAERYPGDADGFIVGGAPTSAYVQTFWA
                     YVAQHVAQDPKRWISPADMARVGQVIMDRFDVSDGARDGMIWDPTRIQLSHALFPFLS
                     AAQYSTLERLAGGLPAIEGSDVSAPGYWLANPALLGPIGLGTAAPPWTDATRPPLFGS
                     TVLSMKALRGPGYDALTQMDFADPRQREAEKAIWDRVGGYGYAPEKLAGMMRNGGKMI
                     MWTGASDEAVPPAYTAHYSAGVRRLYGTPSEDFFQSFFVPGMYHCRGGEGSPTDSSRA
                     MLEAMQRWVEGGHRPTEILMTNAPRELELNSTSNTAMYVSGMSKEKPATAEAPPARSY
                     RICAFPKVAQFTGAAGSDVNDARNWSCTGTMAP"
     gene            6623..7246
                     /locus_tag="VN034_01450"
     CDS             6623..7246
                     /locus_tag="VN034_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV59291.1"
                     /translation="MDVSESRQDGAIRDIGKTGAKIVDGARRCFERYGIEKTTIEDIA
                     KAAGVSRPTVYKFFAGKMDIVDHIGFGELDKIQELVRARIKRELGFADMTTEAIVASV
                     LVASGNPYIRRFVEDLEVSAQSQLPTSPYQRQARRRWESVMKRAQASGELAADLDPAE
                     VVSWLSRNQVMLLRVLDQFGGDEEKLRHMVRRFVVEPLLVNRSTPSS"
     gene            7591..9972
                     /locus_tag="VN034_01455"
     CDS             7591..9972
                     /locus_tag="VN034_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59292.1"
                     /translation="MRSAYKLATCSMAIAVSAISTAAMAQEAEADGAGANEIVVTAQK
                     RDERLQDVPIAVSAFTPEQLQNSGISDVLDLAAVTPSLLITEGSSQAQPYIRGVGGRN
                     ITPGNEATTAVYVDGVYQTDKTGIMLQSFPDVQSVQVLRGPQGTLFGRNATSGAILVT
                     TLQPDQDFGAMLEGTYGTDEEGARGFVTGGLAPNLAIGVSGFYRNERPYVRNRAQAIN
                     GAGKYVGGDESYGVRGQLRWEATPDFSATVAGYYSKGETNNVVALQPILGETTTVGQV
                     ASGIDISRPQRSYYGEIDPEVRFEGYGASLTLDADVGPVNIKSISGYSHSTSGVEYDL
                     DGSPAPVFWFDTGLKSRTWQQEIDITSMNDGPFQWILGGFYLNYRDGYEQLDQYVGLP
                     VPATLRPHTVPQALLDASAAGAGPTSGLAYIDQQAFVTVKSLGVFGEASYQFSEAAKL
                     TLGLRYTDEKHTLDKDNAGTTYVPDGTGGVIAIPRNSAAICTANPACDGLSTPFSELT
                     YRAVFDYKFTDDFMAYASYNRGFKSGVYNISSIPNVIATEPETIDAFEVGFKSKLFDR
                     QVTFNAAAYYYKYDNLQVPVVDPVTNTQQSINAAKATISGMEVELAYNPSDRFSLSAG
                     FATFFKSEYDSFKNCAIYRRAASGLAVPPNADCSGFDLPGTPDISFNIQSTYNVPLAN
                     GGKLELNGLFSYVDQFDHATYGAYPAGGTGAGAYPAGIGRAPFQKATETLNMSVTFHA
                     PDDAFYVSVWGKDLFNQNDIFRNVSTTSFGYYSMLSRGTTAGVTIGTKFGSQR"
     gene            10040..10780
                     /locus_tag="VN034_01460"
     CDS             10040..10780
                     /locus_tag="VN034_01460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWV59293.1"
                     /translation="MATSPGAAGGMLPPDLSAHWYNAEAFVSGEPWRAFDPSRTALVL
                     VDLINWQVDPSGPSIAAIRAAGHPERADHLLARFADTIGPNLELLLPAARRAGVRVVH
                     ARLASRHPDYGDIVPALRPYVQAAGAYDGSLAAMPIAEVGDEPGDLSVVKSGSGAFGG
                     TELDFLLRREGIDTILYAGVVTTACVLLSVAAGFDLGYRQYLIADCTGTLSARDQEDA
                     ERLIGNYLAEIVTAADAVAAMEQQAHAL"
     gene            10770..>11379
                     /locus_tag="VN034_01465"
     CDS             10770..>11379
                     /locus_tag="VN034_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWV59294.1"
                     /translation="MPYDFLTDLLVLEVSHFGPDALGGRLADMGARVIKVEDPDGGDP
                     VRRAGPWSVGEENGFSYLHLRWNRGKESVIIDLASEQGRADFLTLAEKADVVIEGMRA
                     GVMARLGLGYEALKAVNPAIVFCSLSGLGESGPYAKLASHGPSFDAYGGLGTPVGESI
                     SRYDGPQAPSIGMYAYGLEAAFGVVAAVHRARRTGEGAAIEVA"
BASE COUNT         2007 a         3534 c         3785 g         2053 t
ORIGIN      
        1 gtcgagatcg agcttcgcct cgatggtgaa gctggtcgag gggacgggcg gagccagcgc
       61 ctcgggaatg cgcgccgtcg cgccgcgata ggtccagagg aaattgcgcc gcgccatgtc
      121 ggccgccagc tgcttgcgga cgaagcccca ggcctcgctg gtgttggtca gcggatagac
      181 attgttcgcc ttcgcctcgc agtcgaatag cgccttcatc tgggcgagct tgcgcgggtt
      241 cgccgcggcg acattgcgct gttcgccggg gtcggcggcg aggttgtaaa gctcccaatt
      301 tccgtcgctg aacaccggcc ggttcatgaa atcccacacg tcgagccggt gcggcacgac
      361 cgccttccag ccgttcgcat agatcgaccg gttgccgtac atttcataat attgcgtcgc
      421 atgccgcgtc ggcgcggcgg gggcggcgaa gctgtatttc atgctgacgc cgtcgaacgc
      481 catctgccgc tcgccgttga ccgtcgctgc gggagcgatg cccgccgcgt cgagaatcgt
      541 cggggtgatg tcggtaacat gctgatactg gacgcgcagt tcgccgcgcg cggcgatgcc
      601 gcccggccac gacaggatca ggggcacgcg cgtgccgccc tcataggccg tctgcttata
      661 gtaacggaac ggcgtgttgc ccgcgaccgc ccagccgctg gcatagtgag ggtaggtcga
      721 ggggccgccc caatcgtcat aatgacgcag attctcttcg agcgtcgcga aataggcgtt
      781 ggcgaaataa tgctcgctga aagtgccctg cggtgcgcct tcggcgctgg cgccattgtc
      841 ggcggtgatc aggacgaccg tattgtcgag ttcgccgcgc gcctcgagcg cgtcgaggat
      901 acggccgaac tgttcgtcgg cgtggctgag ctgcgcggcg aaggcctcca tctggcgcgc
      961 gaacatgcgc tgctcgtcgc ggctgaggtc ggcccaatgc ttgaccccgg cgggcagcgg
     1021 cgggagcgtc atgtcggcgg ggacgaggcc gagcttcttc tgccggtcga ggatctgctg
     1081 cgccgcgaca tcccagcccg cgtcgaagcg gcccttgtaa tgatcgagcc agtgctgcgg
     1141 ggcatggtgc ggcgcgtgca ccgcgccggt cgcccaatac atcatgaacg gccggtgcgg
     1201 gtcggtcgcg tcgcgcgcgc cgatccagcc gatcgccttt tcggccatgt cggcgctgag
     1261 gtgatagtcg cggcgcggcg acagggtgac gggctgatgg tccgcccaca aggtcgggtc
     1321 gaaattgtcg gcgtcgaagg cgaggaagcc gtaatagcga tcgaagccct gcccgctcgg
     1381 ccaataggtg aaggggcccg cagccgacgc gtcggcctgc ggcagatggt cccacttgcc
     1441 gatcgcatag gtgaggtagc ccgactggcg aagattttcg gcgacggtgc ccgcgctgcg
     1501 cgggacgagc gcatcctgtc cgggaaagcc taccgcgatt gccgaatgcc cgccgatatg
     1561 gacgcgatgc gaattgcgcc cggtgagcag cgccgcgcgg ctcgccgaac agatcggcgt
     1621 cgtatgataa ttggtgtagc gcagcccgcg ctgcgcgacg cggtcgatgt tcggggtttc
     1681 gacgagaccg ccatagctgc cgagctggcc gaaacccatg tcgtcgatca cccagacgag
     1741 gatgttgggc gcccccgcgg gggcggtcgt gcggttgatc ggcccgggga ccgaatcggc
     1801 gaaggtcttg ccgattttgg gctggccgcc gggtgccgcc gacgtcggca aggcgatggc
     1861 cgcagcgacc atgcaggcgc tcgccatgtt gaaaaagggg tggttcctca ttcccataca
     1921 cttcgtgtct gtcgatcgcc ggatgcttga cgatgcacta tatatgaatt acaatcttcg
     1981 taaagtgtaa acattgagat ggcgagggta gggtatgaaa atcgaactgg atgcgatcta
     2041 tcgccgcatt gtcgaccatc tggaaaatgg caccaccgac atggcggcgg attcgatcga
     2101 ggtgcccgcc gcccatttca ccgatgccga ccatctcgcc cgcgagctcg aggtcttccg
     2161 ccgccagccg ctcgctgcgg cgacgagcat ggagattccc gaaccgggca gcttcgtgac
     2221 gcgcgacata ttgggtgtgc ctttgctggt cgtccggcaa aaggacggca gcgtcggcgt
     2281 gttccgcaac atgtgccgcc accgcggcgg caaggtcgag caggccgata aaggcaagaa
     2341 gccctttttc gtctgcgcct atcatggctg gagcttcaag ggtgacggca gtttgcgcgg
     2401 cgtgcccttc gaagagcaat atggcacgct cgacaagggg tgccgcaacc tcttctcggt
     2461 tgcggcggag gaacgccacg gcctcatatg gatcaagctg ccgcaggcgc cgcaggacct
     2521 gggcgttgcc gattatctgg gcgacgccga cgagcggctc gcggcttatg acatcgacca
     2581 gctcgtcgtg ttcatggacc agaagatcga cctcgacatc aactggaagc tggtgatgga
     2641 cggcgcgatc gatgtgctcc acccgcaatt cctgcacgcc gaaggggtcg gcaagctgat
     2701 ccagaccggc gctgcggtgt ggctcgatct cgggcggcac ggccagtcct attcggcgcg
     2761 caagcgcctc gcgcgcaagc tcaaggcggg cgaaccgatc gacaagggtt tccgctacct
     2821 gaccggcaac ctcatgatct ttccgaacat gagcgtgatc ccgacgcccg accatatcga
     2881 gcattggacc gtatggccgc acctgaccga ccccggcaaa tgccacgtcc atatccgttt
     2941 cctgaccgat cccgccaagc tgaccgacga ggtcgcggcg cggatcaacc gcagctggga
     3001 tatcttgcgg caggcggcga ccgaggagga tttcccgatg gaggagatga tccaggccaa
     3061 tgccgcggcg ctgccgacga ccgacttcct ttatggtgcc aacgaagtgc cgacgcagca
     3121 tctgcacctg cagatggcgc gcgagttcga agcgctggcc tgacgcgtgc ctcacggatg
     3181 atgtaaagag agacggctgg ccaacgagcc ggaaccgtct cgggagagac tgcatgaata
     3241 tcgccgaact cgtccgttac tgggcgcgct ggagacccga tcacaccgcc gtcatcgccg
     3301 acgacgtccg cacgagctgg gccgagttcg acgcgcgcag cgatgcgatc gcgcgcggcc
     3361 tgcgcgcggc gggggtgggg aagggcgacc gcgtcggcat gcacctcgac aaccggatcg
     3421 aggcgccgct gctgaccgtc gcatgtctga agcttggcgc gatgatcgtc ccgctgaatt
     3481 tcaagctgac cggcagcgaa atcctgcctt tgctcgagga cgccgattgc aggatcgtcg
     3541 tcagccaggc gaagcatctc gaccggcttg aaatggccgc ggcgaagctg cctttcgcca
     3601 tccacagcct cgacggcagc gatgtgccga gctttgcgtc gctctcgatc ggaaccggcg
     3661 cggtgccgac cgaggccatc ggcctcaatg acgcgggctt cctctgctac acgtcgggga
     3721 cgacgggccg ccagaagggc gcgctgctca cccatggcag cgcgatgtac cccggccttg
     3781 cgaaaaatat cgccgaaggg ctgacctggc gcgattcgat catggtcgcg gtgccctttg
     3841 tcttcaccgg cgcgatcgtg tcctgcttca ttcagttcac ggtcaacgcc ggcggcacga
     3901 tggtgctcga atccgatttc gacatcgacc gctatctcga cgtcatccag cgtcaccggg
     3961 tcagcgccgc gacgacggtg cccgtcgtat gggaacggct gatgcgttcg ccgggattcg
     4021 agcgcgccga tctgtcgagc atgatttcgg ccgccgcggg cggcgcgccg gtcagcatcg
     4081 acctgatcga ggcctatcgc gccaagggca tctcgctgat ccagtcctat ggtctgaccg
     4141 aagcgtcggg cctcgccgcg acgatgcacg gcgacgatgc gatcaagcat atcggctggg
     4201 cgggccgccc gatcgtcggc agcgagatga agatcggcga tgccgacggc catgcgctcg
     4261 ccaatggcga ggtcggcgag atcatggtgc gcggcccgca tgtcatgcgc gaatattggc
     4321 gtaaccccga agccacggcg gcgactttcg cgggcgactg gctccgcacc ggcgacacgg
     4381 ggatgatgaa cgacgaaggt ttcgtgaagg tggtcgaccg gtctaaggac atgatcattt
     4441 cggggggcat caacgtctat ccggccgaga tcgagaagat attgtcggcg aacccgcata
     4501 tcaccgaact cgcggtgatc ggggtgcccg acagcgactg gggcgaagtg ccgatgatcg
     4561 tcgcgagcgg ggttcaggat caggcggccg ccatgtcggc gttcgagtgc gacggggcgg
     4621 cgcagctcgc accgttcaag cggccgcggc gcgtcgtctt catcgacgag ccgctgccgc
     4681 gcaccttgtc gggcaaggtt tccaagccga cgctgcgcgg gcttttcccg gcggttcccg
     4741 aacaggcgaa ggcgttgttc gggaaatagg gcgggttccg gacgtaatga tcgtcgcccc
     4801 gcgaaggcgg gggcgacggc tttaaggcgc catcgtcccc gtgcaactcc agttgcgtgc
     4861 atcgttgaca tcgctcccgg ctgcgccggt gaattgcgca accttgggaa aggcgcaaat
     4921 gcggtagctg cgcgccggcg gggcttcggc ggtcgccggc ttttccttgc tcatccccga
     4981 cacatacatg gcggtgttcg acgtcgaatt gagctcgagc tcgcgcgggg cgttggtcat
     5041 caggatttcg gtcggccggt ggccaccctc gacccatcgc tgcatcgcct cgagcatcgc
     5101 ccgcgaactg tcggtcggcg agccttcgcc gccgcggcaa tgatacatgc cgggcacgaa
     5161 aaagctctgg aagaaatcct cgctgggcgt gccgtaaagg cgccggacgc cggcgctgta
     5221 gtgggccgta taggccggag gcacggcttc gtcggaggcg ccggtccaca tgatcatctt
     5281 cccgccgttg cgcatcatcc ccgccagctt ttcgggggcg tagccatagc cgccgacgcg
     5341 gtcccagatc gctttttccg cttcgcgctg ccgcgggtca gcgaaatcca tctgggtgag
     5401 cgcgtcatag ccgggcccgc gcagcgcttt catgctgagc acggtcgatc cgaacagcgg
     5461 agggcgcgtc gcgtcggtcc atggcggcgc tgcggtcccg agcccgatcg ggccgagcaa
     5521 tgccggattt gccagccaat atccgggtgc ggaaacgtcc gatccctcaa tcgcgggcaa
     5581 tccgcccgca agccgttcga gcgtcgaata ttgcgcggcg ctgaggaaag ggaacagggc
     5641 gtgcgacagc tggatccggg tcggatccca gatcatcccg tcgcgggcgc catcgcttac
     5701 gtcgaagcga tccatgatga cctgtccgac gcgcgccatg tcggcgggcg agatccaccg
     5761 tttggggtct tgcgcgacat gctgcgcaac ataggcccag aatgtctgga cataggcgct
     5821 cgtcggcgcg ccgccgacga tgaagccgtc ggcgtcgccc ggatagcgct cggcctccat
     5881 cagcgtgctg accccgccgc ccgaacagcc catgatatag cgcggcatcc gctgccggtc
     5941 ataatagccg cgcgcgatcg cctgtgtcgc gagggcggcg acatgcgcgc cgcgatgggc
     6001 ataatcctcc gactttccgg ggtcggcgcg gaacgacatg tcgagcgcgc cggtcgaatg
     6061 cgaccccttg tcggtcgacg cgacggcata gcccgcgctg atatgctcgc gcgacgggtt
     6121 gacgatatag ccggcggacc cgccgggaac gaccatcaga tagcggccgt tccagctttg
     6181 cggcagcgcg atcatgaagc cgacgctgtt cggccccgga tcgtcggtgc gcacggtccc
     6241 gtcgatgcgg caataggggg cggggtcgga cagccagcgg accgatccga tcgtgacatc
     6301 ctctcccgcc cagccgatgg cctccggcga acaggcggcc gctgccgtac cgggtatggc
     6361 gaccggccgc gaatcctgcg cggttgcggc ggggaccgtc aggaagaggg tcagtgcggc
     6421 gccgagcgat gatgtccgat actgcatgcc gttgatcctc cacgcgccgg gaattgatac
     6481 tggcatcaaa taacaaaaaa tggaaaatgt aaatattgat gctatgaagg ggcgtagcta
     6541 gcgggcggcg ccggcctttg gcggcgcacc ggctggtcaa cgacccggcg caaagccggg
     6601 caatcggaag gacgaaaagg gcttggacgt ttcggaaagt cgccaggatg gcgccatacg
     6661 cgacatcggc aagaccggcg cgaagatcgt cgacggcgcg cgtcgctgtt tcgagcgata
     6721 cgggatagag aagacgacga tcgaggatat cgccaaggcg gccggcgtct cgcggccgac
     6781 cgtgtataaa ttcttcgccg gcaagatgga tatcgtcgac cacattggtt tcggcgaact
     6841 cgacaagatc caggagctcg tccgcgcgcg catcaagcgc gagcttggct tcgccgatat
     6901 gacgacggag gcgatcgtgg cgtcggtgct cgtcgccagc gggaatccct atatccgtcg
     6961 tttcgtagag gatctcgaag tgtcggctca gagccagctc cccaccagcc cctatcagcg
     7021 acaggcacgg cgccggtggg aatcggtgat gaaacgcgcg caggccagcg gcgaactggc
     7081 cgccgatctc gatccggcgg aggtcgtgag ctggctctcg cgcaatcagg tgatgttgct
     7141 ccgcgtcctc gaccagttcg gcggcgacga ggaaaagctg cgccatatgg ttcgccgctt
     7201 cgtcgtcgag ccgctgcttg tcaatcgatc cacgccgtca agctgaatga aggagaagtc
     7261 gcgccggcga gtcgcgaatc gcccttgacg gaccgccaac gccccatgcc ctgccaagtg
     7321 gcaagtttag gctgttgtgc caagatttgt gaggcttttt agacactaaa ctacataata
     7381 tagattgatc gccgtttccc gcgcattccg cactccgggc agagttgaca ttttattgat
     7441 tttgttgtct gtaacctttc gggagagatg caaggctaaa tggctggtca aggcggtcgc
     7501 tatgatcgcc ggaacagcgc acctcccacg gtgaaggact gcgcggcgtc accctcaaag
     7561 acggtggggc gccgcaaatg ggaggatgtt atgcgctctg cttacaagct cgccacttgc
     7621 tcgatggcga ttgcggtttc ggctatttct actgcagcga tggcccagga ggccgaggcc
     7681 gacggcgccg gagcgaatga aatcgtcgtc acggcgcaga agcgtgacga acgactgcag
     7741 gacgtgccga tcgcggtttc cgccttcacg cccgagcagc ttcagaacag cggcatttcc
     7801 gacgtgctcg atcttgccgc ggtgacgccg tcgctgctga tcaccgaagg gtcgagccag
     7861 gcgcagccct atatccgcgg tgtcggcggc cggaacatca caccgggcaa cgaagcgacg
     7921 acggcggtct atgtcgacgg cgtctaccag accgacaaga ccggcatcat gctccagagc
     7981 tttcccgatg tgcagtcggt gcaggtgctg cgcggcccgc agggcacctt gttcgggcgc
     8041 aacgcgacct cgggcgcgat cctcgtcacc accttgcagc ccgaccagga tttcggcgcg
     8101 atgctcgagg gcacatacgg caccgacgaa gagggcgcgc gtggtttcgt gaccgggggg
     8161 cttgcgccga acctggcgat cggcgtcagc ggcttttatc gcaacgaacg cccctatgtc
     8221 cgcaaccgcg cccaggcgat caacggtgcc ggcaaatatg tcggcggtga cgagagctat
     8281 ggcgtgcgcg ggcagttgcg ttgggaagcg acccccgatt tctcggccac ggtggcgggt
     8341 tattattcga aaggcgaaac caacaacgtc gttgcgctgc agccgatcct cggggaaacg
     8401 accaccgtcg gtcaggtggc gtcgggcatc gacatcagcc ggccgcagcg ttcctattac
     8461 ggcgagatcg atcccgaagt ccgctttgag ggctatggcg cgtcgctgac cctcgacgcc
     8521 gatgtgggcc cggtcaatat caagtcgatc tccggctaca gccacagcac gtcgggcgtc
     8581 gaatatgacc tcgacggctc gcccgcgccg gtcttctggt tcgataccgg cctgaaaagc
     8641 cgaacctggc agcaagaaat cgacatcacg tcgatgaacg acgggccgtt ccagtggatc
     8701 ctcggcggct tctatctcaa ctatcgcgac ggttacgagc agctcgatca atatgtcggc
     8761 ctgccggttc cggcgacgtt gcggccgcac acggtgccgc aggcgctgct cgacgcttcg
     8821 gcggcaggcg ccggcccgac ttcggggctt gcctatatcg accagcaggc gttcgtgacg
     8881 gtgaagtcgc tcggcgtgtt cggcgaagct tcgtaccagt tcagcgaggc ggccaaacta
     8941 acgctcggcc ttcggtacac cgacgagaaa catacgctcg acaaggataa tgccgggacg
     9001 acctatgtcc cggacgggac gggcggggtg atcgcgatac cgcgcaattc ggctgcgatc
     9061 tgcaccgcca atccggcgtg cgacggcctg tcgacgccct tctccgagct tacctatcgc
     9121 gccgtgtttg actataagtt caccgacgat ttcatggctt atgcaagcta caaccggggc
     9181 ttcaagagcg gcgtttacaa tatttcgtcg atccccaatg tcatcgcgac cgagccggaa
     9241 acgatcgacg ccttcgaggt cggattcaaa tcgaagcttt tcgaccgcca ggtgacgttc
     9301 aacgccgcgg cctattacta caaatatgac aatctgcagg tgccggttgt cgatccggtc
     9361 accaataccc agcagtcgat caacgccgca aaggcgacga tttcgggcat ggaagtcgag
     9421 ctggcctata atcccagcga ccgcttctcg ctctcggcag gtttcgcgac cttcttcaag
     9481 tccgagtatg acagcttcaa aaactgcgcg atttatcgcc gcgcggcttc aggtctcgca
     9541 gtgccgccga acgcggactg ctcgggcttc gacctgccgg gcacgcccga tatcagcttc
     9601 aacatccagt cgacctataa tgttccgctg gccaatggcg gcaagctcga gctcaacggc
     9661 ctgttcagct atgtcgatca gttcgaccat gcgacctatg gcgcctatcc ggcgggcggg
     9721 accggcgccg gtgcctatcc ggcgggaatc ggccgcgcgc ccttccagaa ggcgaccgag
     9781 acactgaaca tgtcggtcac tttccatgcg cccgacgatg ctttctatgt atcggtgtgg
     9841 ggcaaggatc tgttcaacca gaacgacatc ttccgcaacg tttcgaccac ctccttcggc
     9901 tattattcga tgctttcgcg cgggacgacg gcgggggtca cgatcgggac gaagttcgga
     9961 tcgcagcggt aggaaaaggg ggcacggccg ccggcacggg cactgccggc ggccgcgttt
    10021 tttgggacgg gagagcgacg tggccacttc cccgggcgcc gcaggcggga tgttgccgcc
    10081 cgacctttcg gcgcattggt ataatgcgga agcattcgtg tcgggcgagc cgtggagggc
    10141 attcgatcct tcccggaccg cgctggttct cgtcgatctg atcaactggc aggtcgaccc
    10201 ctccggaccc tcgatcgccg cgatccgcgc ggctgggcat cccgagcgcg ccgatcatct
    10261 tctggcgcgc ttcgccgaca ccatcggacc caatctcgaa ctgttgttgc ccgccgcgcg
    10321 ccgcgcgggc gtgcgcgtcg tgcatgcgag gctcgcaagc cgccatcccg attatggtga
    10381 tatcgttccc gcgcttcgtc cctatgtgca agcggcgggg gcctatgacg gatcgctcgc
    10441 cgcgatgccg atcgccgagg tcggcgatga accgggcgac ttgtcggtcg tcaaatccgg
    10501 atcgggggct ttcgggggaa ccgagctcga cttcctgctc cggcgcgaag gtatcgatac
    10561 gatcctctat gccggggttg tgacgaccgc ctgcgtgctg ctcagcgttg ccgcggggtt
    10621 cgatcttgga taccggcaat atctgatcgc cgactgcacc ggcacgctct cggcgcgcga
    10681 ccaggaggat gccgaacggc tgatcggtaa ctatctcgcc gaaatcgtga ccgccgccga
    10741 tgccgtcgcg gcgatggagc aacaagcgca tgccctatga ttttctgacc gacctgttgg
    10801 tgctcgaagt ttcgcatttc ggccccgatg cgctcggcgg ccggcttgcc gacatgggcg
    10861 cgcgcgtgat caaggtcgag gatcccgacg gcggcgatcc ggtgcgccgc gcagggccgt
    10921 ggtcggtggg cgaggaaaac ggcttttcat atcttcacct gcgctggaat cgcggcaagg
    10981 aaagcgtgat catcgacctt gcgagcgagc agggcagggc cgatttcctg acgctggcgg
    11041 aaaaggccga tgtcgtgatc gaggggatgc gcgcgggcgt gatggcgcgg ctcgggctgg
    11101 gatatgaggc gctgaaggcg gtcaatcctg cgattgtttt ctgttcgctg tcggggctgg
    11161 gcgaaagcgg tccttatgcg aagctggcct cgcacggccc gtcgttcgac gcctatggcg
    11221 gactcgggac gccggtcggc gaaagcattt cgcgctatga cgggccgcag gcgccgtcga
    11281 tcggcatgta cgcctatggg ctggaggcgg ctttcggggt cgtcgcggcg gtgcatcgcg
    11341 cgcgccggac gggtgagggc gcggcgatcg aggtcgcgg
//
LOCUS       DATMSU010000058         8883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_143070,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000058 DATMSU010000000
VERSION     DATMSU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8883
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143070"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VN034_01470"
     CDS             <1..759
                     /locus_tag="VN034_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWV59295.1"
                     /translation="TAAPAAPAATPSPATTGSIPDFGIPHEDEKLSGMRKTIARRLSQ
                     SMQESPHIYLTVDIRLDALLKLRGELNASLESRGVKLSVNDMLIKALAVALERVPACN
                     VSFGGDVMRKYSRADISVAVSIPGGLITPIIVDAGGKSMSKISTEMSELAAKAKEGKL
                     QPNEYQGGTASISNMGMMGIKQFTAVINPPQAMIMAIGAGEKRPYVVDDALAIATVMS
                     ATGSFDHRAIDGADGALLMKTFKELVENPLGLVA"
     gene            805..1287
                     /locus_tag="VN034_01475"
     CDS             805..1287
                     /locus_tag="VN034_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWV59296.1"
                     /translation="MGSGVRAASAAHKTLSQVLARKAVLLDRARQMRSNPTDAERRLW
                     SILRAGRLDGIRWRRQVIIDDRYIADFICFEHRLIVEADGGQHGENPYDAERDAYLTV
                     QGFRILRFWNNDILANGDGVVASILDAIDSSGAQTRADPTPQPLSRKGRGAFEGAHNG
                     "
     gene            1280..2677
                     /gene="lpdA"
                     /locus_tag="VN034_01480"
     CDS             1280..2677
                     /gene="lpdA"
                     /locus_tag="VN034_01480"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWV59297.1"
                     /translation="MADTNYDLIVLGSGPGGYVAAIRAAQLGLKTAIVERENLGGICL
                     NWGCIPTKALLRSAEIYHYMQHAGDYGLIAQQISADIDAVVKRSRGVAKQLSQGVAFL
                     MKKHKITVHMGEGKLASPGKLEVKGEKGTETLSAKNIIVATGARARDLPFAPADGKRI
                     WTYRHALVPPEMPKKLLVIGSGAIGIEFASFYSDMGAEVTVVEMLDRLVPVEDADVSA
                     FLEKALKKQGMTIHTGAGVEELKATANGVSAKIKGKDGKTQSAEFSHAIVAIGIVPNT
                     ENIGLEALGVKTTKGHIDTDAMCRTNVPGLWAIGDVTAPPWLAHKAMHESIISVEAIA
                     GNHPHAMDPRNIPGCTYCRPQIASVGLTEAKAKELGYEVKAGTFPFIGNGKAIALGES
                     EGFTKTVFDAKTGELLGAHMIGAEVTELIQGYTIGKTAELVEDDFIGTVFPHPTLSET
                     MHEGVLAAFGRPLHI"
     gene            2965..3564
                     /locus_tag="VN034_01485"
     CDS             2965..3564
                     /locus_tag="VN034_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59298.1"
                     /translation="MNPSILHFSRMGSVLKALFFLGMAAIAFAVAGLMHAEREAPPRT
                     VRLPDIELPAPAPYRDPLAPFKVPFLMIAGGVCLFYVGRHGLRGLTRSEAVKIENGAL
                     RFHPSYGVEPNPLPLDAIAEALFDRADRLPGDGPASAKLGARLRHGLYLRCRTDGSME
                     ELRLIDNDIDGGAEQLRRFAAHLDSWRQSAARTARRDRS"
     gene            3600..4211
                     /locus_tag="VN034_01490"
     CDS             3600..4211
                     /locus_tag="VN034_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWV59299.1"
                     /translation="MAAAIALILAVIALGFAVGAGWTQDADWQVSHLLSLRIDESEPA
                     FIAFMQGASWIGGGTPRWIIVILLCALVWHWCGPRCAAALGGASLLSNLASSLLKLGF
                     GRARPDLIDHLDHVSSASYPSGHATNAAVVYLLLAWLAPPRWRPFAWTLAAAMIILNG
                     FSRIMLGVHWASDIAGGTMLGTAFALLGAWWVGLSSRQTAING"
     gene            4221..5141
                     /locus_tag="VN034_01495"
     CDS             4221..5141
                     /locus_tag="VN034_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWV59300.1"
                     /translation="MAQGTHDFIPDPRNDAILINVNGTLVPRAQASVSVFDSGFMLGD
                     GVWEGIRVHKGRMAFLDQHLDRLWEGAKAIAMDIGITRDALERRLYDTIDANGMDGCH
                     IRLMVTRGIRSTPYQDPRVVVSPATIVIIAEHKDPLPATVEKGLSLFTVHVRRGDPAV
                     QDPKLNSHSKLNCITACIQAAQAGADEALMLDPHGFVATCNSTHFFIVRKGEVWTSSG
                     DYCLGGITRGNVIRICRENGIPVYEKNFSLTDVYGADEAFVTGTFAGVVPAHTIDGRE
                     LTKGRGPMVERLQGLYKALIERDIAGQSRP"
     gene            5138..5875
                     /locus_tag="VN034_01500"
     CDS             5138..5875
                     /locus_tag="VN034_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWV59301.1"
                     /translation="MTDCVRIAMWSGPRNLSTAMMRSFGSRADSFVSDEPFYGAYLKQ
                     TGDPQPMIDAVMASMDCDWHGIARTMTGPCPTGAKLWYQKHMAHHMVGPIAYDDLSGL
                     RHAFLIRDPARVIASYAAKRVSVRPDHLGTAKQVEFFDREADRLGHAPPVIDSADILR
                     DPSAMLQKLCAALGIAWDPAMLRWKAGIHDTDGVWASHWYDAVASSTGFGPPDKPQPE
                     LTAEGRAVADACRPGYDYLARHKITAG"
     gene            5946..7280
                     /locus_tag="VN034_01505"
     CDS             5946..7280
                     /locus_tag="VN034_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV59302.1"
                     /translation="MQSRVKFGSFLRGIIFASTALAGAAALPVQAQDGAGQTVITAAR
                     YVDVLTGKAVDNPAIFVGADGRITNIADARTVRWGSNVKHVDLGDKTLLPGLIDMHVH
                     LDGPADIGGYRGLEFTDSFWGMTAVKNANDMLGAGFTTVRNVGSSDRNDIGLKQAIDA
                     GYATGPRIVPAGYALGATGGHCDSTFLPPSLEKEDGKEEGIGDTPDELRYQVRRQRKY
                     GAEVIKVCATGGVFSRNTEPGQLQVSEKELAAIADEAHQWGLRVAAHAHGAEGIRAAI
                     AAGIDTIEHVSLVDDEGIRMAVARKRPVWFSMDIYNTEYTQAEGAKNGVLEDNLRKDR
                     EIAQIQRDNFRKAVKAGVRMVFGSDAGVMPHGDVGKQFRVMVEYGMTPMQAIQAATKN
                     AAEALGREQDVGAIAVGRYADIVAVDGDPLTDVRQLESVDAVVKGGVLVRGE"
     gene            complement(7285..8064)
                     /locus_tag="VN034_01510"
     CDS             complement(7285..8064)
                     /locus_tag="VN034_01510"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HWV59303.1"
                     /translation="MIAERVLPLAGVHNFRDYGGYAVEGGGRLRGGTLWRSAHHEAAT
                     DEDLTALDALGLETVVDLRGDDERELHPCRRSDNFSARVLFAGGVTAGLAPHLQAAGG
                     TIDVATARERMIDTYAGMPYRPALVATLRLYLAALSEYDAPSLVHCVAGKDRTGFAVA
                     IVHRLLGVHEDDLMHDYLLTNTAGKIEERIAQGAAHIRSRYGAEIHDDAIRALMSVNP
                     SYLDAALATVRRDHGDIPTYAEAVLNFTPEMREAMLDRLVV"
     gene            complement(8067..8870)
                     /locus_tag="VN034_01515"
     CDS             complement(8067..8870)
                     /locus_tag="VN034_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWV59304.1"
                     /translation="MGEFLSVERRGKIAILTMIKPESMNAIGTHEDCQDIIDTLRTLG
                     DDRSVSAIILTGSGKAFSAGGNLKGMQDRTGIGVLDQPDSTRANYRKGVQAVIRALMD
                     CEVPMIAAVNGHAIGLGCDLACTCDIRIAAESAKFACSFIKVGIVPGDGGAWLLQKVL
                     GYPRAAELFLTGDRFDAVQAKEYGLVTDVVPDAELLDRAIAIAERIVCNPPRALRLTK
                     RLLREAQHSRMSDILELSAAYQAIVHETADNREAINAFVEKRPPVFTGE"
BASE COUNT         1614 a         2920 c         2864 g         1485 t
ORIGIN      
        1 acggctgctc ccgccgcccc ggcagcaaca ccgtcaccgg ccaccacggg cagcatcccc
       61 gatttcggca tcccgcacga ggacgaaaag ctcagcggca tgcgcaagac gatcgcgcgc
      121 cgcctcagcc agtcgatgca ggaatcgccg cacatctacc tgaccgtcga catccgcctc
      181 gacgcgctcc tgaaactgcg cggcgagctc aacgccagcc tcgaaagccg cggcgtcaag
      241 ctcagcgtca acgacatgct gatcaaggcg ctcgcggtcg cgctcgaacg cgtccccgcg
      301 tgcaatgtca gcttcggcgg cgacgtgatg cgcaaatata gccgcgccga catctcggtc
      361 gcggtgagca tcccgggcgg gctgatcacc ccgatcatcg tcgacgcggg cggcaagtcg
      421 atgtcgaaaa tctcgaccga aatgtccgaa ctcgcggcaa aggcgaagga aggcaagctc
      481 cagcccaatg aatatcaggg cggcaccgcc tcgatctcga acatgggcat gatggggatc
      541 aagcaattca ccgcggtgat caacccgccg caggcgatga tcatggcgat cggcgcgggc
      601 gagaagcggc cctatgtggt cgacgacgcg ctcgcgatcg cgaccgtcat gtcggcgacc
      661 ggcagtttcg atcaccgcgc gatcgacggc gccgacggcg cgctgctgat gaaaaccttc
      721 aaggaactgg tggagaaccc gctggggctg gtggcgtaaa tgcccgagag gggccaaagc
      781 ccctctcccc ttgcgggaga ggggttgggg agtggggttc gcgccgcgtc cgcggcgcac
      841 aagactcttt cccaagtcct cgcccgcaaa gccgtgctgc tggaccgcgc ccgccagatg
      901 cgatcgaatc ccaccgatgc cgaacgaagg ctctggtcga tcctccgcgc cggcaggctg
      961 gacggcatca ggtggcggcg ccaagtgatc atcgacgatc gctatatcgc cgacttcatc
     1021 tgcttcgaac atcggctgat cgtcgaagca gacggcggcc agcatggcga aaatccttat
     1081 gacgccgaac gcgatgccta tttgaccgta caaggctttc gcattctgcg cttctggaac
     1141 aacgacatcc tcgccaacgg cgatggcgtc gtcgcgagca tcctggatgc catcgattct
     1201 tcgggcgcgc agacgcgcgc cgaccccaca ccccaacccc tctcccgcaa ggggagaggg
     1261 gcttttgaag gagcccataa tggctgacac caactacgac ctcatcgtcc tcggctcggg
     1321 tcccggcggc tatgtcgcgg cgatccgcgc ggcgcagctc ggcctgaaga ccgcgatcgt
     1381 cgagcgcgag aatctcggcg gcatctgcct caactggggc tgtatcccga ccaaggcgct
     1441 gctgcgctcg gccgaaatct atcattatat gcagcatgcg ggcgattacg gcctgatcgc
     1501 gcagcagatc agcgccgata tcgacgcggt cgtgaagcgc agccgcggcg tcgcgaaaca
     1561 gctgagccag ggcgtcgcct tcctgatgaa gaagcacaag atcaccgtcc atatgggcga
     1621 gggcaagctc gcgagtccgg gcaagctcga ggtcaagggc gagaagggaa ccgaaactct
     1681 ctccgcgaag aacatcatcg tcgcgaccgg cgcccgcgcc cgcgacctgc cgtttgcccc
     1741 cgccgacggc aagcgcatct ggacctatcg ccatgcgctc gtgccgcccg aaatgccgaa
     1801 gaagctgctc gtcatcggat cgggcgcgat cgggatcgaa ttcgcaagct tctacagcga
     1861 catgggggcc gaagttacgg tggtggagat gctcgaccgg ctcgtccccg tcgaggatgc
     1921 cgacgtctcg gccttcctcg aaaaggcgct gaagaagcag ggcatgacga tccacaccgg
     1981 cgccggcgtc gaagaactga aagcgaccgc gaacggcgtc tcggcgaaga tcaaaggcaa
     2041 ggacggcaag acccaatccg ccgagttcag ccatgcaatc gtcgcgatcg gcatcgttcc
     2101 gaataccgag aatatcggcc tcgaagcctt gggcgtgaag acgaccaagg gtcatatcga
     2161 caccgacgcg atgtgccgca ccaacgtccc cggcctctgg gcgatcggcg acgtcaccgc
     2221 gccgccgtgg ctcgcgcaca aggcgatgca cgaatcgatc atctcggtcg aggcgatcgc
     2281 gggcaaccac ccgcacgcga tggacccgcg caacatcccg ggctgcacct attgccgccc
     2341 gcagatcgcc agcgtcgggc tgaccgaggc aaaggcgaag gaactcggtt acgaggtcaa
     2401 ggccggcacc ttccccttca tcggcaacgg caaggcgatt gcgctcggcg agtccgaagg
     2461 tttcaccaag accgtgttcg atgcgaaaac aggcgagttg ctcggcgcac acatgatcgg
     2521 cgccgaagtc accgagctga tccagggcta tacgatcggc aagaccgcgg aactggtcga
     2581 agatgatttc atcggcacgg tcttcccgca tccgacgctc agcgagacga tgcacgaggg
     2641 cgtgctcgcc gcgttcggac gtccgctgca tatctagggc caggacccgt taattgcgcg
     2701 ttcgaggttt gaagcgattt tgctcagggc taggaggcga ggcgtaggaa tatgtcaata
     2761 tttcaagact tgccgacgaa gccatgggca aaatcggtca aatccccctt tcccgcttat
     2821 tggggggacg tcgaaatggc ttcgatctcg ggcccgcgcg gccttgcggg tagcggtgct
     2881 acccgctgca tccacccgaa cccgatatct tcgccatttc gacgcctcga acgtgcgatt
     2941 aacgggtcct ggccctaggc gcgcttgaac ccgtcgatcc ttcatttctc ccggatgggc
     3001 agcgtcctca aggcgctgtt cttcctgggg atggcggcga ttgctttcgc cgtcgccggc
     3061 ctgatgcacg ccgagcgcga agcaccgccg cggaccgtcc gcctgcccga catcgaactg
     3121 cctgcgccgg cgccgtatcg cgacccgctg gcgccgttca aggtcccgtt cctgatgatc
     3181 gcgggcgggg tttgcctctt ctatgtcggc cggcacggcc tccggggcct cacgcgaagc
     3241 gaggcggtga agatcgaaaa cggagccctt cgctttcacc cgagctatgg cgtcgaaccg
     3301 aacccgctgc cgctcgatgc catcgccgag gcgcttttcg accgcgccga ccggctgccg
     3361 ggcgatggcc cggcatcggc gaagctgggc gcccgcctgc ggcatggcct ttaccttcgc
     3421 tgccgcaccg atggttcgat ggaggaatta cgcctgatcg acaatgacat cgacggcggg
     3481 gcggagcagt tgcggcgttt tgcagcccat ctggacagct ggcgccaatc ggcggcgcga
     3541 acggcaaggc gagacaggtc gtgacgccgg tcgtgacgag ggccggcaag caaaggcagg
     3601 tggctgccgc gatcgcgctg atccttgccg tcatcgcgct cggctttgcg gtcggtgcgg
     3661 gctggacgca ggatgcggac tggcaagtgt cgcatctgct ctcgctgcgc atcgacgaaa
     3721 gcgaacccgc attcatcgcc ttcatgcaag gcgcaagctg gatcggcggc ggcaccccgc
     3781 gctggatcat cgtcatcctg ctctgcgcgc tcgtctggca ctggtgcggg ccgcgctgtg
     3841 cggccgcgct cggcggcgcc tcgctgcttt cgaacctcgc gtcgagcttg ctcaagctcg
     3901 gtttcggccg cgcgcggccc gatctgatcg atcatctcga ccatgtatcg agcgcatcct
     3961 atccgagcgg ccacgcgacg aacgcggcgg tcgtctacct gctcctcgcg tggctcgcgc
     4021 cgccgcgctg gcgccccttc gcctggacgc tcgccgcggc aatgatcatc ctcaacggct
     4081 tctcgcgcat catgctgggc gtccattggg caagcgacat cgccgggggc acgatgctcg
     4141 gcaccgcctt cgcgctgctc ggcgcgtggt gggtcgggct ttcctctcga cagacggcga
     4201 tcaacggcta acctcgcggc atggctcaag gcactcacga tttcatcccc gatccgcgca
     4261 acgacgcgat cctgatcaac gtcaacggga ccctcgttcc ccgggcgcag gcaagcgtat
     4321 cggtgttcga cagcggcttc atgctcggcg acggcgtgtg ggagggtatt cgcgtccaca
     4381 aggggcgcat ggcgttcctc gaccagcatc tggatcgcct atgggaaggc gcaaaggcga
     4441 tcgcgatgga tatcgggatc acgcgcgacg cgctcgaacg gcgcctttac gacacgatcg
     4501 acgcgaacgg catggacggt tgccatatcc ggctgatggt gacgcgcggc atccgctcga
     4561 cgccctatca ggacccgcgc gtcgtcgtct cgcccgcgac gatcgtgatc atcgccgagc
     4621 acaaggatcc gctccccgcg acggtcgaga aggggctgtc gcttttcacc gtccatgtcc
     4681 ggcgcggcga ccccgcggta caggacccca agctcaattc gcattcgaag ctcaattgca
     4741 tcaccgcgtg catccaggcg gcgcaggccg gcgccgacga ggcgttgatg ctcgacccgc
     4801 acggtttcgt cgcgacgtgc aattcgacgc acttcttcat cgtgcgcaag ggcgaggtgt
     4861 ggacgtcgag cggcgattat tgcctcggcg gcatcacgcg cggcaatgtc atccgtatct
     4921 gccgcgaaaa cggcattccg gtctatgaga agaatttctc gctcaccgac gtctatggcg
     4981 ccgacgaggc gtttgtgacc gggaccttcg ccggcgttgt cccggcgcat acgatcgacg
     5041 ggcgcgaact gaccaagggc agggggccga tggtcgagcg gctacagggg ctctacaaag
     5101 cgctgatcga gcgcgacatt gcggggcagt cccggccgtg acggactgcg tccgcatcgc
     5161 gatgtggtcg ggaccgcgca acctctccac tgcaatgatg cgcagctttg gcagccgcgc
     5221 cgacagcttc gtcagcgacg aacccttcta tggcgcctac ctgaagcaaa ccggcgatcc
     5281 gcagcctatg atcgatgcgg ttatggcgtc gatggattgc gactggcatg gcattgcacg
     5341 aacgatgacc ggcccgtgcc cgaccggcgc gaaactctgg taccagaagc atatggcgca
     5401 ccacatggtc ggcccgatcg cctacgacga cctttccggc ctgcgccacg ccttcctgat
     5461 ccgcgatccc gcgcgcgtga tcgcaagcta tgcggcaaag cgcgtttcgg tgcgccccga
     5521 tcacctcggc accgcgaagc aggtcgaatt tttcgaccgc gaggccgatc ggttgggcca
     5581 cgcgccgccg gtgatcgaca gcgccgatat attgcgcgat ccgtccgcga tgctgcagaa
     5641 attgtgcgcg gcactcggca tcgcgtggga ccccgcgatg ctgcgctgga aagccgggat
     5701 tcacgacacc gacggcgtct gggcctcgca ctggtacgat gccgtcgcgt cgagcaccgg
     5761 cttcggtccg cccgacaaac cgcaacccga actgacggcg gaggggcggg cggtcgccga
     5821 tgcctgccgt cccggttacg actatctggc acggcataaa atcaccgccg ggtgaaaatt
     5881 ctgctgtcct attaagtccc gttttgccat ccattttgcg ggcactgaca aggggcggga
     5941 aaaacatgca atcgcgagta aagttcggca gttttctgcg gggaatcatc tttgcttcga
     6001 cggcgctggc gggagccgct gcgctcccgg tgcaggcgca agacggcgcg ggtcagaccg
     6061 tcatcaccgc tgcgcgttac gtcgacgtgc tgaccggcaa agcggtggac aatcccgcga
     6121 tcttcgtcgg cgccgacggc cgcatcacga atatcgccga tgcgcgaacc gtgcgctggg
     6181 gatcgaacgt caagcatgtc gacctcggcg acaagacgct gctgccgggg ctgatcgaca
     6241 tgcacgtcca tctcgacggc cccgccgaca tcggcggcta tcgcgggctc gaattcaccg
     6301 acagcttctg gggcatgacc gcggtcaaga atgcgaacga catgctcggc gcgggcttta
     6361 cgaccgtgcg caacgtcggg tcgagcgacc gcaacgatat cgggctcaag caagcgatcg
     6421 acgccggcta tgcgaccggc ccgcgcatcg tccccgcggg ctatgcgctg ggcgcgaccg
     6481 gcgggcattg cgacagcacc ttcctgccgc cgagcctcga gaaggaggac ggcaaggaag
     6541 agggcatcgg cgatacgccc gacgagcttc gctatcaggt ccgccgtcag cgcaaatatg
     6601 gcgcagaggt gatcaaggtc tgcgcgaccg gcggtgtctt ttcgcgcaac accgagccgg
     6661 gacagcttca ggtgtcggag aaggaactcg ccgcgatcgc cgacgaggcg caccaatggg
     6721 ggctgcgcgt cgccgcgcac gcgcatggcg ccgagggcat ccgcgcggcg atcgcggctg
     6781 ggatcgatac gatcgagcat gtcagcctgg tcgatgacga gggcattcgc atggcggtgg
     6841 cgcgcaagcg gccggtgtgg ttctcgatgg atatctacaa caccgaatat acacaggccg
     6901 aaggcgcgaa gaacggcgtg ctcgaggata atctgcgcaa ggatcgcgag atcgcgcaga
     6961 tccagcgcga caatttccgc aaggccgtaa aggcgggcgt gcggatggtg ttcggttcgg
     7021 acgcgggcgt gatgccgcac ggcgacgtcg gcaagcagtt tcgcgtcatg gtcgaatatg
     7081 ggatgacgcc gatgcaggcg atccaggccg cgacgaagaa cgccgccgag gcgcttggcc
     7141 gcgagcagga cgtcggcgcg atcgcggtcg gccgctatgc cgacatcgtc gcggtcgacg
     7201 gcgatccgct gaccgatgtg cgccagctcg aaagcgtcga tgcggtggta aagggcgggg
     7261 tgctggtaag gggcgaatag gcccctagac caccagccgg tcgagcatcg cctcgcgcat
     7321 ctcgggagtg aagttcagca ccgcctcggc ataggtcggg atgtcgccat ggtcgcggcg
     7381 caccgttgcc agcgcggcgt cgagatagga ggggttgacc gacatcagcg cgcggatcgc
     7441 gtcgtcgtgg atctcggcgc cgtatcgcga gcggatatgc gccgcgccct gcgcgatgcg
     7501 ttcctcgatc tttcccgcgg tgttggtgag cagataatcg tgcatcagat catcctcatg
     7561 gacgccgagc agccggtgaa cgatcgcgac cgcaaagccg gtgcgatcct tgcccgcgac
     7621 gcagtggacg aggctgggcg cgtcatattc ggacagcgcc gcgagataga gccgcagcgt
     7681 cgcgacaagc gcggggcgat agggcatgcc cgcataggtg tcgatcatcc gctcgcgcgc
     7741 cgtcgcgacg tcgatcgtac cgcccgccgc ctgcagatgc ggggcgagac ccgcggtcac
     7801 cccgcccgcg aacagcacgc gcgccgagaa attgtccgac cgccggcacg ggtgcagctc
     7861 gcgctcgtcg tcgccgcgca ggtcaacgac cgtctcgagc cccagcgcgt cgagcgcggt
     7921 caaatcctcg tcggtcgccg cctcatgatg cgccgagcgc cacagcgtcc cgccgcgcag
     7981 cctgccgcca ccctcgaccg cgtaaccgcc atagtcgcgg aaattgtgga cgccggccag
     8041 tggcaggact cgctcggcaa tcatcatcat tctcctgtaa acacgggggg acgcttttcg
     8101 acgaacgcat tgatcgcctc gcggttatcc gcggtttcat ggacgatcgc ctgataggct
     8161 gcgctcaact ccaaaatatc gctcatccgg ctgtgctgcg cctcgcgcag caaacgcttc
     8221 gtcaggcgca gcgcccgggg cgggttgcag acgatccgct cggcgatggc gatcgcccgg
     8281 tcgagcagtt ccgcatcggg cacgacgtcc gtcaccaaac catattcctt tgcctgcacc
     8341 gcgtcaaagc ggtcgccggt caggaacagc tcggcggcgc gcggatagcc aagcactttc
     8401 tggagcagcc aggccccgcc gtcgccgggg acgatcccga ccttgatgaa gctgcacgcg
     8461 aatttggcgc tctccgccgc gatgcggatg tcgcaggtgc acgcaaggtc gcagccgagc
     8521 ccgatcgcat ggccgttgac cgccgcgatc atcggcacct cgcaatccat cagcgcgcgg
     8581 atcaccgcct gcacgccctt gcgataattg gcgcgcgtgg aatcgggctg gtcgagcacg
     8641 ccgatgccgg tgcgatcctg catgcccttg aggttcccgc cggcgctgaa cgccttgccg
     8701 ctgccggtca ggatgatcgc gctgacgctg cggtcgtcgc caagggtgcg cagcgtgtcg
     8761 atgatgtcct ggcaatcttc gtgggtgccg atcgcattca tgctctcggg cttgatcatc
     8821 gtcaggatcg cgatcttgcc ccgccgctcg acactcagaa attcgcccat cttcaattat
     8881 cct
//
LOCUS       DATMSU010000059         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_19969, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000059 DATMSU010000000
VERSION     DATMSU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19969"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VN034_01520"
     CDS             complement(<1..1161)
                     /locus_tag="VN034_01520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWV59305.1"
                     /translation="MLGMDLPETVPQASSGPYRVLARKYRPQTFAELIGQDAMVRTLG
                     NAIARDRLAHAFLMTGVRGVGKTSTARLIAKALNCIGPDGQGGPTIDPCGVCEPCRAI
                     TEGRHIDVIEMDAASNTGVDDVREIIEAVRYAAVSARYKIYIIDEVHMLSRNAFNALL
                     KTLEEPPPHVKFLFATTEVNKVPVTVLSRCQRFDLRRITPDMLFAHFSSILQKEGVEA
                     EAPAIWLIANAAEGSVRDGLSILDQAIAHADLDGGGKISADQVRVMLGLSDRTSIAQL
                     MATILDGDAGGAIDLARAQYALGIEPVAMVRGLMDLTHAITLAKVSRHDDPALAASDR
                     ERIGDWAQKLGFAPLNRLWQLLLKGHDEVLRANNPLEHLEMLLLRVIYAASMP"
     gene            complement(1235..1332)
                     /gene="ffs"
                     /locus_tag="VN034_01525"
     ncRNA           complement(1235..1332)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VN034_01525"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1428..1766)
                     /locus_tag="VN034_01530"
     CDS             complement(1428..1766)
                     /locus_tag="VN034_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWV59306.1"
                     /translation="MMRVTFIHADGKARTEAEAKPGDILLDVAQAHLMPLEGTCEGQM
                     ACSTCHVIVAKEDFDRLPPASEMEEDMLDLAAGVRRTSRLSCQIVLTDDLDGLTVHIP
                     AESRNMQGPR"
     gene            complement(1763..>2687)
                     /locus_tag="VN034_01535"
     CDS             complement(1763..>2687)
                     /locus_tag="VN034_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWV59307.1"
                     /translation="VAALLPKGGRVFFTSGATEALNWALLRGAEAMPGGVAGLSIEHA
                     ASLQCLERLNAAILPVDSEGLALAPDDTSIPENGIVATMLVNNEVGTIQPVADFAAAA
                     HAKNSLLLCDAVQGYGRVAIPEGPDLIALSAHKVHGPKGIGALWVRDGVDLPPLMFGG
                     AQEQGMRSGTVSPALCAGFGAAAALAAERFDKDAEHIKRLWSLARDMLPEWTINGDAN
                     RRYHGNLNVRREGVNGLRLMSDARDVAFSLGSACGSGSGKVSHVLRAMGVSEADARAS
                     IRLGWGRYTSEQELRDGLNAIKDAARLQGVN"
BASE COUNT          440 a          896 c          900 g          451 t
ORIGIN      
        1 gggcatcgac gcggcataaa tcacgcgcag caacagcatt tcgagatgtt cgagcgggtt
       61 gttcgcgcgc agcacctcgt catgcccttt gagcaatagc tgccagagac gattgagcgg
      121 cgcgaagccg agcttttgtg cccagtcgcc gatgcgttcg cggtccgatg ccgcaagcgc
      181 ggggtcgtcg tggcgcgaga cctttgccag cgtgatcgcg tgggtcaggt ccatcaaccc
      241 gcgcaccatc gcgacgggct cgatgccgag cgcatattgc gcgcgcgcaa ggtcgatcgc
      301 tccgcccgcg tcgccatcga gaattgtcgc catcaattgc gcgatggaag tgcgatcgga
      361 caggccgagc atgacgcgca cctgatcggc gctgatcttc ccgccgccgt cgaggtcggc
      421 gtgcgcgatc gcctggtcga gaatcgacag gccgtcgcgc accgaaccct cggcggcatt
      481 ggcgatcagc cagatcgccg gcgcctcggc ctcgacgccc tctttctgaa ggatcgagct
      541 gaaatgcgcg aagagcatgt cgggggtgat gcggcgcagg tcgaaacgct ggcagcgcga
      601 cagcaccgtc accggcacct tgttgacttc ggtggtcgcg aacaggaatt tgacgtgcgg
      661 cggcggctct tcgagtgttt tcagcagcgc attgaaggcg ttacgcgaca acatgtgcac
      721 ttcgtcgatg atgtaaatct tgtagcgcgc cgacaccgcg gcgtagcgca cggcctcgat
      781 aatctcgcgc acatcgtcga cgccggtgtt cgacgcggcg tccatttcga tcacgtcgat
      841 atggcggccc tcggtgatcg cgcggcacgg ttcgcacacg ccgcacggat cgatcgtcgg
      901 cccgccctgc ccgtcggggc cgatgcagtt caaagccttg gcgatcaggc gcgcggtcga
      961 ggtcttgccg accccgcgca cgccggtcat caggaacgca tgcgccagcc gatcgcgcgc
     1021 gatcgcattg ccgagcgtgc gcaccatcgc atcctgcccg atcagctcgg cgaaggtttg
     1081 cgggcgatat ttgcgggcca agacgcggta cggaccgctc gatgcctgcg gcaccgtctc
     1141 gggcaaatcc attcccagca tcggggtatc gtcgtcggcg gaatcgtcca tggccccgtc
     1201 ttaggggcgc gccgcggtct tgtcgaaggg ggaaatgggt ggaagccgga acgacccgcg
     1261 gcgaattcgt tgcggctgct tccttccgga cctgaccggg ttggcgaaga ccacgtccgc
     1321 ccgacttccg cggcgcatat ggcggcgcgc gcaggaaaat gcaagggcgg tggggatcaa
     1381 gatgatcgtc accccggatc aagtccgggg tgacgaaaga agcaagatca gcgtgggccc
     1441 tgcatgttgc ggctttcggc agggatatgc accgtcagcc cgtcgagatc gtcggtcagc
     1501 acgatctggc acgacaggcg gctggtgcgg cgcaccccgg cggcgaggtc gagcatatct
     1561 tcctccatct cgctcgcggg cgggaggcgg tcgaaatcct cttttgcgac gatgacatgg
     1621 caggtcgagc aggccatctg gccctcgcac gtcccttcga gcggcatcag atgcgcctgc
     1681 gcgacgtcga gcaggatatc cccgggcttc gcctcggcct cggtgcgcgc cttgccgtcg
     1741 gcgtggatga aggtgacgcg catcagttca cgccctgcaa tcgcgcggcg tccttgatgg
     1801 cgttgaggcc gtcgcgcaat tcctgctcgc tcgtgtagcg accccaaccg aggcggatcg
     1861 aggcgcgcgc gtcggcctcg ctgaccccca tcgcgcgcaa cacatggctg actttgcccg
     1921 acccgctgcc gcacgcgctg ccgagcgaga aagcgacgtc gcgcgcatcg gacatcaggc
     1981 ggaggccgtt cacgccctcg cgccggacgt tgagattgcc gtgatagcgg cggttcgcat
     2041 cgccattgat cgtccattcc gggagcatgt cgcgcgcgag cgaccagagc cgcttgatat
     2101 gctcggcatc cttgtcgaac cgctcggcgg cgagtgcggc cgcggcgccg aagcccgcgc
     2161 agagcgcggg cgaaacggtg cccgagcgca ttccctgttc ctgcgcgccg ccgaacatca
     2221 gcggcggcag gtcgacgccg tcccggaccc acagcgcgcc gatgcccttg ggcccgtgga
     2281 ccttgtgcgc cgagagcgcg atcaggtccg ggccctcggg gatcgcgacg cggccataac
     2341 cctgcaccgc gtcgcagagc agcaggctgt tcttcgcatg tgccgcggcg gcgaaatcgg
     2401 cgaccggctg gatcgtaccg acctcgttgt tcacgagcat cgtcgcgacg atgccatttt
     2461 cggggatcga cgtgtcatcg ggggccagcg ccagcccttc gctatcgacc ggcaggatcg
     2521 cggcgttcag ccgctcaagg cactgcagcg acgccgcatg ttcgatgctg agccccgcga
     2581 cgccgcccgg catggcctcc gcgccgcgca gcagcgccca gttgagcgct tcggtagcgc
     2641 cggaagtaaa gaacacccgc ccgcccttcg gcagcagcgc cgcgacc
//
LOCUS       DATMSU010000060         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_124478,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000060 DATMSU010000000
VERSION     DATMSU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124478"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VN034_01540"
     CDS             <1..132
                     /locus_tag="VN034_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWV59308.1"
                     /translation="SFIFEITGTSDKVDRFIALMRECGLVEVGRTGVVAIARGSEAL"
     gene            230..1270
                     /gene="ilvC"
                     /locus_tag="VN034_01545"
     CDS             230..1270
                     /gene="ilvC"
                     /locus_tag="VN034_01545"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HWV59309.1"
                     /translation="MQVYYDRDADQDLIKGKKVAVVGYGSQGHAHAQNMRDSGVKEVA
                     IALRPGSATAKKAEAAGFKVLSNKEAAEWADVIMIAAPDEHQAKIYADDIGPNMKPGA
                     ALAFAHGLNIHFGLIDARPDIDVFMVAPKGPGHTVRSEYQKGGGVPCLIAVAQEAQGA
                     GSSGNGFAKALALSYASAVGGGRSGIIETTFKEECETDLFGEQAVLCGGITHLIQAGF
                     ETLVEAGYAPEMAYFECLHETKLIVDLLYEGGIANMRYSISNTAEYGDIKTGPRIITD
                     ETKKEMKRVLADIQSGRFVKDFVLDNQAGQPELKASRKAAAAHQLEKTGAELRAMMPW
                     IAKNQLVDKSKN"
     gene            1311..1910
                     /locus_tag="VN034_01550"
     CDS             1311..1910
                     /locus_tag="VN034_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6151 family protein"
                     /protein_id="HWV59310.1"
                     /translation="MASDLSFACDCGTVTGAMHVIAGDGEHIVCHCTDCQNLAHHLGH
                     AERLLDAHGGTSLYQTRCARMRIDTGRDRLACVHMTDGSTLRWYAACCRIPLFNTVAT
                     GKLPFITVQLGACDPAARDRLLGPPRGHLFTHEATGDASALPKMSMVTLMRRFFVRAI
                     KDIVSGDRRRSALFDAKTLEPIAKPHRLTDEEKLGLPHR"
     gene            1962..>2113
                     /locus_tag="VN034_01555"
     CDS             1962..>2113
                     /locus_tag="VN034_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWV59311.1"
                     /translation="MPGLSPFTAHHADLAALAHDSRRRTLAPRAGRDFASNDYLGLAD
                     SETLRSA"
BASE COUNT          415 a          742 c          633 g          323 t
ORIGIN      
        1 agcttcatct tcgaaatcac cgggacgagc gacaaggtcg accgcttcat cgcgctgatg
       61 cgcgaatgcg ggctggtcga ggtcggccgc accggcgtcg tcgccatcgc ccgcgggtcg
      121 gaggcgctct gagtcaccgt catcccagcg aaagctggga tcgcacgcag tttaacaccg
      181 agaccgatcc cagcttgcgc tgggatgacg aataataggg aagaaagaca tgcaggttta
      241 ttatgatcgc gacgccgacc aggatctgat caagggcaag aaggtcgccg tcgtcggcta
      301 tggcagccag ggccacgcgc acgcgcagaa tatgcgcgac agcggcgtca aggaggtcgc
      361 gatcgcgctc cgccccggtt cggcaaccgc caaaaaggcc gaagccgcgg gcttcaaggt
      421 gctgtcgaac aaggaagccg ccgaatgggc cgacgtcatc atgatcgccg cccccgacga
      481 gcatcaggcg aaaatctacg ccgacgacat tggcccgaac atgaagcccg gcgcggcgct
      541 cgccttcgcg cacggcctca acatccactt cggcctgatc gacgcgcgcc ccgacatcga
      601 cgtcttcatg gtcgcgccca agggccccgg ccacacggtg cgcagcgaat atcagaaggg
      661 cggcggcgtc ccctgcctca tcgcggtcgc acaggaagcg cagggcgcgg gttcgtcggg
      721 taacggcttc gcgaaagcgc tcgccctctc ctacgcgtcc gcagtgggcg gcggccgcag
      781 cggcatcatc gagacgacct tcaaggaaga gtgcgaaacc gacctcttcg gcgaacaggc
      841 ggtgctctgc ggcggcatca cccacctgat ccaggcgggt ttcgaaacct tggtcgaggc
      901 gggctacgcc cccgaaatgg cctatttcga atgcctccac gaaaccaagc tgatcgtcga
      961 cctcctctat gaaggcggca tcgcgaacat gcgctattcg atctcgaaca ccgccgaata
     1021 tggcgacatc aagaccggcc cgcgcatcat cactgacgaa acgaagaagg aaatgaagcg
     1081 cgtcctcgcc gacatccagt cgggccgctt cgtcaaggac ttcgtgctcg acaaccaggc
     1141 cggccagccc gaactgaagg cgagccgcaa ggcggccgcc gcgcatcagc tcgaaaagac
     1201 cggcgctgaa ctccgcgcga tgatgccgtg gatcgccaag aaccagctcg tcgacaagtc
     1261 gaagaactga cggcctttcg caatcgcggg cgaggagcta gggccggatc atggcatccg
     1321 atctctcctt cgcctgcgac tgcggaaccg tcaccggcgc gatgcacgtc attgcgggcg
     1381 atggcgagca tatcgtctgc cactgcaccg attgccagaa cctcgcccac catctcggcc
     1441 acgccgaacg cctgctcgac gcgcacggcg gcacctcgct ctaccagacg cgatgcgcgc
     1501 gcatgcggat cgacaccggc cgcgaccggc tcgcctgcgt ccatatgacc gacggatcga
     1561 cgctgcgctg gtatgcggca tgctgccgca taccgctctt caacaccgtc gcgacgggca
     1621 agctgccctt catcacggtc cagctcggcg cctgcgatcc ggcggcgcgc gaccggctcc
     1681 ttggcccacc gcgcggccat ctcttcacgc acgaggcgac cggcgacgcg agcgcgctgc
     1741 ccaaaatgag catggtcacc ttgatgcggc gctttttcgt ccgcgcgatc aaggacatcg
     1801 tctcgggcga ccggcggcgg tcggcgctct tcgacgcaaa gaccctcgaa ccgatcgcca
     1861 aacctcatcg attgaccgac gaagaaaagc ttggccttcc gcaccgctga gccggcacac
     1921 gcgcgtttgt cattttcatc agatgcccta agggaacccc gatgccgggc ctctctccct
     1981 tcaccgccca ccacgccgat ctcgcggcgc tcgcgcatga cagccgccgc cgcaccctcg
     2041 cgccgcgcgc cggccgcgat ttcgcgtcga acgactatct cggccttgcc gatagcgaaa
     2101 cgctccgttc cgc
//
LOCUS       DATMSU010000061         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_133797,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000061 DATMSU010000000
VERSION     DATMSU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133797"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..>3273)
                     /locus_tag="VN034_01560"
     CDS             complement(2..>3273)
                     /locus_tag="VN034_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59312.1"
                     /translation="APPADDSAAASDEIVVVGVRAALQDAQDRKRDALTVVDSITATD
                     IGAFPDNSVASALQRVPGITVSRLQSTDDSTHPSGEPAGVLIRGLTFVRTEINGRDSF
                     SADSYRGLNFNDISPELMSRVDAYKNQTADMIEGGIAGTVDLRTRLPLDEKGLVVAAN
                     AKATYGDRSDQWNGEFSVLVSKTFDTDIGRFGLMADYARSHLVTRTESVIMDKIRTYC
                     SAGAVDAEGNGIVNPDGSVACTANPFGGTGWAYLPDGIRYSQVDYDRTREGIALAGQY
                     ESPTGDVRATVQFIKSSYDNAWLENVSHAKLEGTYYGTPAFNPVGSSILRTPTGNAGF
                     TFGPDGMLRSATLSQGYGSWRGSFGSEAEMIDTGSAVPGLPFVNYCGPDSACAVPDRN
                     GLYFENEARNFDHRESTRDLSANIQWDVTDRFKLNFDAQWIKAKTTNDDILVATASAA
                     DYEYSVNEDGTPNIKLLPGTNINYADGGLANPHNYWMPFIQAHLEDNDAEELALKADA
                     EYDFDGNDWIDSLKVGVRYADRNQNVRYSTFNWTPIAQPWNCNGPGFNVDNTSAAPYP
                     AGCGARPGFNGYGEGLFEPVSLGGGFYNGGVFDNGELVYLSRDALRDRDRLVDGLRGS
                     NVNPPILPGWTPICDRTEATVGCFTPSEVMEVTEKTKAAYAMLGFGGDNATLGGMNVR
                     GNIGVRVVRTDIDSVGNVGFPQSSIFTPVLNRPCGTPLPPGDVVNPACFLTPEIQAFA
                     NGAAVPNNLKADYTTVLPSFNVRLGLDDNNYIRFAYSRAMARPDFGLLRNFVAIQTPS
                     FDASADSPNIVRDGAGNITGYNFTFRAESGFAGLKPILADQFDLTFEHYRGSTSFHAD
                     IFYKKLRNTVAYGEFERLFTNNGSEQTVLMRGPRNVKDGGELYGFETGFQTFFDFLPG
                     LLSGLGAQANYTHVRQSGISNSNLVTQGALDGGGTGGFGAGLDVSGGRGVVMDSHRLA
                     GISKHTFNLVGLYEKGPVAFRVAYNWRSRFLTNNLDCCIGLPVFQKSVGFLDASLRIS
                     PTPWLELSVEGQNLLNTTTVYQQQIFGDTPLTPGAKPVYRDANWGRVDRRFQFGARVK
                     F"
BASE COUNT          569 a         1011 c         1104 g          589 t
ORIGIN      
        1 atcagaactt cacgcgcgcc ccgaactgga agcggcggtc gacgcggccc cagttcgcgt
       61 cgcgatagac gggcttcgcc cccggggtca gcggcgtgtc gccgaagatc tgctgctgat
      121 agaccgtggt ggtattgagc agattctgac cctcgaccga cagctcgagc cacggcgtcg
      181 gcgagatgcg cagcgacgcg tcgaggaagc cgaccgattt ctggaacacc ggcaggccga
      241 tgcagcagtc gagattgttg gtcaggaagc gcgagcgcca attgtaggcg acgcggaagg
      301 cgaccggccc cttttcatag aggccgacga ggttgaaggt gtgcttcgaa atgccggcga
      361 ggcggtgcga atccatcacc acaccgcgcc cgcccgacac gtcgagcccc gcaccgaagc
      421 cgccggtgcc gccgccgtcg agcgcgccct gcgtcaccag gttcgaattg ctgatcccgc
      481 tctgccggac gtgggtgtag ttcgcctgcg cgccgaggcc gctgagcagg ccgggcagaa
      541 agtcgaagaa ggtctggaag cccgtttcga aaccatagag ctcgccgccg tccttcacgt
      601 tgcgcggacc gcgcatcagc accgtctgtt cggacccgtt gttggtgaag agacgctcga
      661 attcgccata agccacggtg ttgcgcagct ttttataaaa gatgtcggcg tggaaggagg
      721 tgctgccgcg ataatgttcg aaggtgaggt cgaactggtc cgccaggatc ggcttcagtc
      781 cggcaaagcc cgattcggcg cggaaggtga aattatagcc ggtgatgttg cccgcaccgt
      841 cgcggacgat gttggggctg tcggcggacg catcgaacga cggcgtctgg atcgcgacga
      901 aattccggag cagaccgaaa tcgggccgcg ccatcgcccg cgaataggcg aagcggatat
      961 aattattgtc gtcgagcccc aagcggacgt tgaagctggg caggaccgtc gtgtagtcgg
     1021 ccttcagatt gttcggcacg gcggcgccgt tggcgaaggc ctgaatctcg ggggtcagga
     1081 aacaggccgg attgacgacg tcgcccggcg gcagcggcgt cccgcacggc cggttgagca
     1141 ccggcgtgaa gatcgaggat tgcgggaatc cgacattgcc gacgctgtcg atgtcggtgc
     1201 ggacgacgcg cacgccgata ttgccgcgga cattcatccc gccgagcgtc gcattgtcgc
     1261 cgccgaagcc cagcatcgca taggccgcct tggtcttttc ggtgacctcc atcacctccg
     1321 acggcgtgaa gcaaccgacc gtcgcctcgg tccggtcgca gatcggggtc cagccgggca
     1381 gaatcggcgg gttgacgttc gatccgcgga ggccatcgac gagccggtcg cggtcgcgca
     1441 gcgcatcgcg gctcagatag acgagttcgc cattgtcgaa gacgccgccg ttatagaaac
     1501 cgccccccag gctgaccggt tcgaacagcc cctcgccata gccgttgaac ccgggacgcg
     1561 cgccgcagcc ggcgggatag ggtgccgccg acgtgttgtc gacgttgaac cccggcccgt
     1621 tacagttcca cggctgcgcg atcggcgtcc agttgaaggt cgaatagcgg acattctggt
     1681 tgcggtcggc atagcggacg ccgaccttca gcgaatcgat ccagtcattg ccgtcgaaat
     1741 catattcggc gtcggccttg agcgcgagtt cctcggcgtc attgtcctcg agatgcgcct
     1801 gaatgaaagg catccagtaa ttgtgcggat tggcgaggcc gccgtcggca tagttgatgt
     1861 tggtgccggg cagcagcttg atgttcgggg tgccgtcctc gttgacgcta tattcgtaat
     1921 cggcggccga cgcggtcgcg acgagaatgt cgtcgttcgt cgtcttggcc ttgatccatt
     1981 gcgcatcgaa attcagcttg aaccggtcgg tcacgtccca ctggatgttc gccgacaggt
     2041 cgcgcgtgct ttcgcgatgg tcgaaattgc gcgcctcatt ctcgaaataa aggccgttgc
     2101 gatcgggaac ggcgcaggcc gaatcgggac cgcaatagtt gacgaagggc agcccgggca
     2161 ccgccgagcc ggtgtcgatc atctcggctt cgctgccgaa gctgccgcgc cagctgccat
     2221 agccctggct gagcgtcgcc gagcgcagca tgccgtcggg gccgaaggtg aagcccgcat
     2281 tgcccgtcgg cgtgcggagg atcgacgatc cgaccggatt gaacgccggc gtgccgtaat
     2341 aagtgccctc aagcttcgcg tgcgagacat tttcgagcca cgcattgtcg taggacgact
     2401 tgatgaactg caccgtcgcg cggacgtcgc ccgtcgggct ttcatattgc cccgcgagcg
     2461 cgatcccctc gcgcgtgcgg tcgtaatcga cctgcgaata gcggatgccg tcgggaagat
     2521 aggcccagcc cgtgccgccg aacgggttcg cggtacaggc gacgctgccg tcgggattga
     2581 cgatcccgtt gccctcggca tcgaccgcgc cggccgagca ataggtgcgg atcttgtcca
     2641 tgatcacgct ttcggtccgc gtgacgaggt ggctgcgcgc atagtcggcc atcaggccga
     2701 agcggccgat gtcggtgtcg aaggtcttgc tgaccagcac cgaaaattcg ccgttccact
     2761 ggtccgaccg gtcgccatag gtcgctttcg cattggcggc gacgaccagc cctttctcgt
     2821 cgagcggcaa gcgcgtgcgc agatcgacgg tgccggcgat gccgccctcg atcatgtcgg
     2881 cggtctggtt cttataggca tcgacgcgcg acatcagttc gggcgagatg tcgttgaagt
     2941 taagcccgcg atagctgtcg gccgagaagc tgtcgcggcc gttgatctcg gtccgcacga
     3001 aggtgaggcc gcggatcagc acgccggcgg gctcgcccga ggggtgggta ctgtcgtcgg
     3061 tcgactgcag ccgcgaaacg gtgatgccgg gaacgcgctg aagcgcgctc gccaccgaat
     3121 tgtccgggaa ggcgccgatg tcggtcgcgg tgatcgaatc gacgacggtc agcgcgtcgc
     3181 gcttgcggtc ctgcgcgtcc tgcaacgccg cgcgcacccc gacgacgacg atctcgtcgc
     3241 tcgccgccgc gctgtcgtcg gcgggcggcg cat
//
LOCUS       DATMSU010000062         1927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_152914,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000062 DATMSU010000000
VERSION     DATMSU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152914"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1554
                     /gene="ubiB"
                     /locus_tag="VN034_01565"
     CDS             10..1554
                     /gene="ubiB"
                     /locus_tag="VN034_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HWV59313.1"
                     /translation="MTRPVTHIVRLLKWGRILARHGALRGIESAPTTPPGVKRLCRIA
                     RFGTIQPKIPDYATAFQAIGPAAVKLGQTLATRPDLVGEEAARNLLTLQDALAPVALD
                     RIRQEIEATFEAPLESLYSEFDPEPVGSASIAQVHRAVTTDGRQVAVKVRRPGIDKQF
                     ARDIETYEWAAAHLEAFGGEAARLRPRQVIANFRRWTLRELDLRREAASASELADAMT
                     AVPEYEVPAIDWDRTASRVMTMSWIDGVKISHRDRLVTAGHDMEALASNLVHAFLRQA
                     IAEGFFHADMHQGNLFVKADGTIAAVDFGIMGRINRQARYWLAEILYGLTTGNYRRVA
                     EIHFEAQYVPDYHSVEEFATALRAVGEPMRGKPVSELSVGQMLDGLFAITRDFDMQTQ
                     PHLLLLQKTMVMVEGVATMLNPRINMWDVSGPFVSAWIRDELGPEAALADGIREQGKT
                     LALIPDIIRRLDAQLPKKGAAPPAPPLPEIPLMWDKGEKRRWWRYALTALAGAAAGAG
                     LFQWLG"
     gene            1559..>1927
                     /locus_tag="VN034_01570"
     CDS             1559..>1927
                     /locus_tag="VN034_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWV59314.1"
                     /translation="MTQPRILLVIGGGIAAYKSIELVRLLRKAGMIVRCVITRAGEQF
                     VTPLTLAALSENKIYTHLFDLKDEVEMGHIQLSREADLVVVAPATADLLAKMAAGIAD
                     DLATTLLLATDKPVLAAPAMN"
BASE COUNT          331 a          650 c          638 g          308 t
ORIGIN      
        1 gtggaaagct tgacccgacc cgtcacccat atcgtccgcc tgctgaagtg ggggcgtatc
       61 ctcgcgcgcc acggcgcgct gcgcgggatc gagagcgcgc cgacgacccc gccgggggtc
      121 aagcgcctgt gccggatcgc gcgcttcggc acgatccagc cgaaaatccc cgattacgcc
      181 accgccttcc aggcgatcgg ccccgccgcg gtcaagctcg gccagacact cgcgacgcgc
      241 cccgacctcg tcggcgaaga ggccgcgcgc aacctgctga cgctgcagga cgcacttgcc
      301 cccgtcgcgc tcgaccggat ccggcaggaa atcgaggcga ccttcgaagc gccgctcgaa
      361 agcctctata gcgagttcga ccccgagccc gtcggttcgg cgtcgatcgc gcaggtccac
      421 cgcgcggtga cgaccgatgg gcgccaggtc gcggtcaagg tgcgccgccc gggcatcgac
      481 aagcagttcg cgcgcgacat cgaaacctat gaatgggccg ccgcgcatct cgaggccttt
      541 ggcggcgaag ccgcgcgctt gcgtccgcgg caggtgatcg ccaatttccg ccgctggacc
      601 ttgcgcgaac tcgacctgcg ccgcgaagcg gcctcggcat cggagctcgc cgacgcgatg
      661 acggcggtgc ccgaatatga ggtgccggcg atcgattggg atcgcaccgc gagccgcgtg
      721 atgacgatga gctggatcga cggcgtcaaa atctcgcatc gcgaccggct cgtcacggcg
      781 gggcacgaca tggaggcgct ggcgtcgaac ctcgtccacg cttttctgcg gcaggcgatc
      841 gccgagggat ttttccacgc cgacatgcac cagggcaatt tgttcgtgaa ggccgacggc
      901 acgatcgccg cggtcgattt cggcatcatg gggcggatca accggcaggc gcgctattgg
      961 ctcgccgaaa tcctctatgg gctgaccacg ggcaattacc gccgcgtcgc cgagattcat
     1021 ttcgaggcgc aatatgtccc cgactatcat agcgtcgagg aattcgcgac ggcgctgcgc
     1081 gcggtcggcg agccgatgcg cggcaagccg gtgagcgaac tcagcgtcgg gcagatgctc
     1141 gacgggctgt tcgcgatcac acgcgatttc gacatgcaga cacagccgca cctgctcctg
     1201 ctccagaaga cgatggtgat ggtcgagggt gtcgcgacga tgcttaatcc caggatcaac
     1261 atgtgggatg tatcggggcc cttcgtcagc gcgtggattc gcgacgaact cggccccgaa
     1321 gccgcgctgg ccgacgggat ccgcgaacag ggcaagacgc tcgcgctgat ccccgacatc
     1381 atccgccgcc tcgacgcgca attgccgaag aagggcgccg ccccgcccgc cccgccgttg
     1441 cccgagattc cgctgatgtg ggacaagggc gagaagcgcc gctggtggcg ctatgcgctg
     1501 actgcgctgg ccggcgccgc ggctggtgct ggcctgtttc aatggttagg ttagcgacat
     1561 gacccagcct cggattctgc tcgttatcgg cggcggcatc gccgcgtaca agagcatcga
     1621 gctcgtccgc ctgctccgca aggcgggcat gatcgtgcgc tgcgtgatca cgcgcgcggg
     1681 cgaacagttc gtcacgccgc tgacgctcgc ggcgctcagc gagaacaaga tttacaccca
     1741 tctctttgac ctgaaggacg aggtcgagat ggggcatatc caattgagcc gcgaggccga
     1801 cctggtcgtc gtcgcgcccg cgacagccga tcttttggcc aagatggcgg cgggcatcgc
     1861 cgacgacctt gcgaccacgc tgctgctcgc gaccgacaag ccggtgctcg ccgcgcccgc
     1921 gatgaat
//
LOCUS       DATMSU010000063         7032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_48767, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000063 DATMSU010000000
VERSION     DATMSU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48767"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1267
                     /locus_tag="VN034_01575"
     CDS             89..1267
                     /locus_tag="VN034_01575"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HWV59315.1"
                     /translation="MAKAHKPGALLRERVEHQHARVSYVELFFDLVFVFAITQISHTL
                     LEHFSPLGVAQAAFLLGAVWWIWVYTSWVTNWLDPERPPVRILLFVLMFLGLVMSCAL
                     PEAFGANGLAFAGAYVTAQLVRSIFTMVSLKDASPANYRNFQRITIWLVCSGAFWIAG
                     GLSEGPLRFGLWLVALGIEYVGPAAGFRVPGLGHSRTSDWNVEGGHLSERCALFIIIA
                     LGESVLMTGATAAKLPVTAVNLTAFLNSFLSSVAMWWIYFHIGAERASHHIARSDDPG
                     RIARIAYTYLHVLLVAGIILVAVGDELVIAHPTGHTDGRTAIAVLAGPAFYLLGNLLF
                     KRITAGWYPLSHLVGLGLLAVLAPAWTVLPPLGFSLAATAILILVAAWEAVSLRAKEA
                     "
     gene            1290..1580
                     /locus_tag="VN034_01580"
     CDS             1290..1580
                     /locus_tag="VN034_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV59316.1"
                     /translation="MDNRKEPFRAHEWETPHGRLVTTIMAGFAEFERSLILARTSEGL
                     SRAKARGVKLGRPFGMTRHQRQEALARKAAGEPVREIARSYNVSPSTISRLL"
     gene            complement(1732..2700)
                     /locus_tag="VN034_01585"
     CDS             complement(1732..2700)
                     /locus_tag="VN034_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HWV59317.1"
                     /translation="MRAVFYETNGPARDVLRIGDVETPSPGVGEVRVRLYASGVNPSD
                     VKSRGHRKSPFPLIIPHSDGAGEIESIGPGVAKSRLGERVWIWNGQWQRPFGTAAEFI
                     ALPAAQAVPLPAALSFEEGAALGIPALTAYHAVELAGVGQGTTLLVSGGAGAVSQFAI
                     QFAKARGATVISTISSPDKAMAAREAGADHCIDYKRENVGDRIADITGRRGVDAITEM
                     DLSANAGLIPAVLQPKGKVIVYGTGAEATIPAFAFLANSIQLNFFLIYQLDAQQRERA
                     VAGVTRALAQGEAVARIGPTFPLSETAAAHEAVEGGTIGNVIVKIE"
     gene            complement(2821..3804)
                     /locus_tag="VN034_01590"
     CDS             complement(2821..3804)
                     /locus_tag="VN034_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWV59318.1"
                     /translation="MDPALPKLLQFDRFALDLTRERLRAGDQEIALRPKAFEVLRHLA
                     ENAGRLVSKRELFEVVWPNVAVTDDSLVQCIRELRDKLGDTDHLLIETVPRRGYLLNA
                     GHPRAAKEHDSLPPPERSQTPIVGSVGRRLVLWSPVQRKRASLATAAITICCLAAAGY
                     LTAHLSLPSSANALFTEDDARRITAIAAEKELPLPPFQISRIGRGVLPEHRRFVGIWV
                     SSTGFVNSNRQFMLVITGAEPSGIVTGFTVRGPPRALSLVVKPAGATHFKARVLGDSF
                     RYSGPNSEREVVMTADRRLEFNEVFDTGVIVRVALDPVWALVEAEQKAALW"
     gene            4144..4275
                     /locus_tag="VN034_01595"
     CDS             4144..4275
                     /locus_tag="VN034_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59319.1"
                     /translation="MSTTKFITAALTLCSAVRAFACLSRILRIAGHQDGAPTVSCAI"
     gene            4783..5853
                     /locus_tag="VN034_01600"
     CDS             4783..5853
                     /locus_tag="VN034_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV59320.1"
                     /translation="MAAGTSTPVLTMFDVVRAEALEDSSGKIRVIERAAQSNRTYTYQ
                     LQDRALWHPRFEPPIFTLATRGAGSLLTSFVASSFSTEVTVDGDEGSLYCFTTMLAGH
                     ITLEQKGRSTTATQQTGLTWRPGPGGRLLISDDNARTNVFLKAADIESTLEHMLDERL
                     RRPLIFEPSLDWGTGLTASLKSQLDFVTQEFQRRDGVANNPVALASLTDLLVSLILRG
                     TTHNYSDRLANAAGNPIPAYVRRAEDFMRAHGAEPIRMSHIAAAAGCSLRTLTAVFRH
                     FRGSTPLAALHAVRLDLAHADLARGIRDASVATVARRYGFTNTARFRTAFHRRFGETP
                     IEIARYASIRTAPASADPAKDE"
     gene            complement(5993..7032)
                     /locus_tag="VN034_01605"
                     /pseudo
     CDS             complement(5993..7032)
                     /locus_tag="VN034_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611561.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1316 a         2212 c         2103 g         1401 t
ORIGIN      
        1 acttccgcca ttgacggcga tacgggctgc gggacaccct cgctgacggg ctgcgcgcga
       61 aagcgcgcag caggggatag gggtctgcat ggccaaagct cacaaacccg gcgcgctgct
      121 cagggaacgc gtcgaacacc agcatgcgag ggtctcctac gtcgagctgt tcttcgacct
      181 ggtcttcgtc ttcgcgatca cccagatttc gcacacgctg ctggagcatt tcagcccgct
      241 cggcgtcgcg caggccgcct ttctgctcgg cgccgtctgg tggatctggg tctatacgag
      301 ctgggtcacg aactggctcg atcccgagcg ccccccggtc aggatcctgc tcttcgtcct
      361 gatgtttctc ggcctggtga tgtcctgcgc cttgcccgag gccttcggtg cgaacggcct
      421 cgcctttgca ggcgcctatg tcaccgccca gctcgtccgc tcgatcttca cgatggtgag
      481 cctcaaggac gcctcgcccg ccaattaccg caacttccag cgcatcacga tctggctcgt
      541 ctgctccggt gcattctgga tcgccggtgg cttgagcgaa ggccctctgc gcttcggcct
      601 ctggctggtc gcgctcggca tcgaatatgt cggcccggcc gccggcttcc gcgttccagg
      661 ccttgggcac tcgcgcacca gcgactggaa tgtcgaaggc ggtcatctct ccgagcgctg
      721 tgccctcttc atcatcatcg cgctcggtga atccgttctg atgacgggcg cgaccgccgc
      781 gaagctgccg gtgacggccg tcaacctgac agccttcctc aattccttcc tgagctcggt
      841 cgcgatgtgg tggatttatt tccatatcgg tgccgaacgg gcgagccatc acatcgcacg
      901 ctccgacgac cccggccgga tcgcacgcat cgcctatacc tatctgcacg tgctgctggt
      961 cgccggcatc atcctggtcg ccgtcggcga cgaactggtc atcgcccatc ccaccggcca
     1021 taccgatggc agaacggcaa tcgccgtcct ggccggcccc gccttctatc tgctcggcaa
     1081 cctgctgttc aaacgcatca cggccggctg gtatccgctc tcccatctcg tcggcctcgg
     1141 cttgctcgcc gtgctggcac cggcctggac ggtgttgcca ccgttgggct tcagcctggc
     1201 cgcaaccgcg atcctgatcc tggtcgccgc ctgggaagcg gtctcgctgc gggccaagga
     1261 ggcctgatcc atgcacacca tcaatttcat tggataatcg gaaagagccc tttagagcac
     1321 atgaatggga aacgcctcat ggccgcctcg tcacaacaat catggccggg ttcgcagagt
     1381 tcgagcgctc gttgattttg gcgcgaacca gcgagggcct tagccgcgct aaggcccgag
     1441 gcgtgaaact cggccggccg ttcggaatga cccgccatca gcggcaagag gccctggcta
     1501 ggaaagctgc cggcgaacct gttcgagaga tcgcacgtag ctacaatgta agcccgagca
     1561 cgatatcgcg gcttctctaa cgccgcgcga cgtgggatcg caatcgacag gccgcatggg
     1621 aggctgcgct gggtaatcta accgatgtag gcgacaccga taggaactct tcgacaatca
     1681 ggaaggggcc gaaggatcga aggcgtaaga gatcgcctag ccacgctgcg attactcgat
     1741 tttgacgatg acattgccaa tcgttccgcc ctcaactgct tcgtgcgcgg cggcggtttc
     1801 gctgagcggg aatgtcggtc cgatacgggc aaccgcttcg ccctgagcaa gagcgcgcgt
     1861 gacgccagcc actgcacgtt ctcgctgctg cgcgtccaat tgatagatca ggaagaagtt
     1921 caactggatc gaattggcga ggaaggcaaa ggcaggaatc gtcgcttcgg ctcccgtgcc
     1981 gtaaacaatg acctttccct ttggttggag taccgccgga atcagaccgg cgttggccga
     2041 taggtccatc tcggtgatag cgtcgacgcc gcgtctcccc gtgatatcag caatacggtc
     2101 gcccacattt tcacgcttat agtcgatgca atggtcggcg ccggcttcgc gcgctgccat
     2161 cgccttgtcc ggcgatgaga tcgtgctgat cacggtcgcg ccccgagcct tggcgaattg
     2221 aatggcgaac tggctcaccg cccctgcgcc gccggatacg agcaaggttg tgccctgacc
     2281 gaccccggca agctcgacag catggtaggc cgtcagcgca gggatgccga gagcggcgcc
     2341 ctcctcgaag ctaagtgcag ctggcaaggg aacggcctgc gcggccggca gtgcaatgaa
     2401 ctcggcggct gttccaaatg ggcgctgcca ctgcccgttc cagatccaga cccgctcgcc
     2461 gaggcgtgac ttcgcaacgc caggaccaat agattcgatt tctccagcac cgtcgctatg
     2521 tgggatgatc agcgggaacg gcgactttcg gtgtccgcgg cttttgacat cggatggatt
     2581 gacccccgac gcgtacaagc gcacgcgaac ctccccaact cccgggcttg gagtttcaac
     2641 gtctccgatg cgcagcacat cgcgcgctgg cccgttggtc tcatagaata ctgcacgcat
     2701 cgatcagtat ccctcagcaa tgtgagagcc tggaagaacg atagctctgt gcggcccggc
     2761 caagcgctag cgacagccgg acggaccata gcgacaatgg tgctcgctgt gccgcgccgg
     2821 ctaccacaga gcagcttttt gctcggcctc aaccagtgcc cacaccgggt cgagagcgac
     2881 acgcacgatc actcccgtgt cgaagacctc gttgaattca agccgccggt cggctgtcat
     2941 caccacctcg cgctcgctgt ttggaccact gtagcgaaag gaatcgccga ggacgcgggc
     3001 cttaaaatgg gtcgcgccag ccggcttgac gacaaggctc agggcccgag gaggccctcg
     3061 aactgtaaag cccgtcacga tgccggaagg ttcggctccg gtgatgacca gcatgaattg
     3121 gcggttcgaa ttaacgaacc cggtgctgct gacccagatg ccgacgaagc ggcgatgttc
     3181 agggagaaca ccacgcccga tccggctgat ctggaatggc ggaagcggaa gctccttctc
     3241 tgctgcaatt gccgtaatcc gccgcgcgtc gtcttctgtg aagagagcgt tggctgacga
     3301 tggcaacgac aggtgggctg taagatatcc agctgcagcc aggcagcaga tggtgatcgc
     3361 ggccgtcgcc agagacgccc gtttgcgctg tacaggcgac cataaaacga ggcgacgacc
     3421 aactgacccg acaatcgggg tttgagatcg ctcgggtggc ggcagcgagt cgtgctcttt
     3481 ggccgcgcgg gggtggccgg cgttcagcag gtatccccga cggggtacgg tttcgatcag
     3541 gagatgatcg gtgtcaccca gcttgtctcg cagctcgcga atgcactgaa cgagcgagtc
     3601 atccgtgaca gcgacatttg gccatacgac ctcaaacagt tcccgcttcg acaccagccg
     3661 tccagcattc tcggcaaggt gacggagaac ctcgaacgcc ttgggccgta gtgcaatctc
     3721 ctgatcgccc gctcttaggc gttcgcgcgt caaatccagc gcaaatcggt cgaactgtag
     3781 aagtttcgga agcgccgggt ccatggtgct tagctcccac cctagcccgc tcgatgaact
     3841 gcgtccgccg actataggcg aaatatcggc attccgtcac ccattgaccg tgaccaaacg
     3901 gaatgctctt cgctattaac gacttcggag gttggcggcg gggcgcctcg tcatccttgg
     3961 ggtgatgaac tggtcgccgg aatggtatcg ccccagcaag cacgcggtcg acgatctggc
     4021 gcggcagttc gctgcatttt taagcagaca cggcgatatt tcgccgagca gagcctcgag
     4081 ttgattgcaa tcagggcaat gaaggcggct tgatcacaac ggctacgcgg ccggcaggcg
     4141 acgatgagca ccaccaaatt catcacggcg gcgctcacgc tctgcagtgc ggtccgggcc
     4201 ttcgcctgtc tgtcgcggat tttgcgcatt gccgggcatc aggatggagc gccaaccgtt
     4261 tcctgtgcga tctgaggcct gctcggcact ttgttcaaag ccggtccccg gacgaggagt
     4321 tccctgcggc tcgccatgat gaccgtgctg tgatcggcat gacgatgccc ctgtcgcagc
     4381 ccgcaggacc gatctggttg acgaactcac cacggcgtga attctgcgga gcagttcgag
     4441 aggacagatc gacgcatttc gccggggcag cctgcgtcaa gacagggcgg gaaatctctc
     4501 cgagtgcagg aaagaagcca gcgacgatca cgatcaactc cagcatttca aaaactccag
     4561 ttgcattaac attcttattc gtcggatttg agctggttaa tcttgaggcg gaaccgtagc
     4621 gcgggagatg cttacgctgc gtcgcccata tgtgctaagt ttgctgcggt ggggaacgac
     4681 gctttcaact acgctccaag tgcgccgccg aatttgtcgg accgttgccg aatttgtcta
     4741 aaattgcttg cgtcacagta agaaagctgg acggagacgc ctatggccgc gggcacgagc
     4801 acccctgtgc taacaatgtt cgacgttgtt cgagccgaag ctctggagga ttcgagcggc
     4861 aagattcgcg ttattgaacg ggccgcacag tcaaaccgca cctacaccta ccaattgcag
     4921 gatcgtgctc tttggcaccc acgcttcgag ccgccgattt ttacccttgc gacacgtggc
     4981 gccggctctc ttctgacgag ttttgtcgcg agcagtttta gcaccgaggt caccgtcgat
     5041 ggcgatgaag ggagcctgta ttgtttcacg acgatgctcg cgggccatat aaccttggaa
     5101 cagaagggaa gatccacgac agccacacaa caaaccggtc tcacttggag gcccggtccc
     5161 ggcggtcgcc ttttaatcag cgacgataac gcgcgcacca atgttttctt gaaggctgcg
     5221 gatatcgaga gcacgcttga acacatgctg gatgagcggt tgcgtcggcc gctgattttc
     5281 gagcccagcc tcgattgggg caccgggctt acagccagcc tgaaaagcca actcgatttc
     5341 gttacacagg aatttcaacg gcgggacggt gttgccaata acccagtcgc acttgcgtcg
     5401 ctgacggacc tactcgtctc gctgattttg cgtggcacca ctcataatta ctccgatcga
     5461 ctcgcaaatg cagccggaaa tccaattccg gcctatgttc gacgcgcgga agacttcatg
     5521 cgcgcccacg gcgcggagcc tatccgtatg tcacatatcg ctgcggccgc aggctgcagc
     5581 ttgcgaacct tgacggctgt gttcaggcat tttcgcggca gcaccccttt ggctgcattg
     5641 catgctgtcc ggcttgatct agcgcatgcc gatctggctc ggggcatacg cgacgcctcg
     5701 gtcgctaccg tcgcccgccg atacggcttc actaacacgg cacgctttag aaccgccttt
     5761 catcgccgat tcggtgagac tcctattgaa atcgcccgat atgcgtccat acggacggcg
     5821 ccggcctccg ccgatcctgc aaaagacgaa tgaccggttg cggtttgatc gatcctggca
     5881 gcgcgcaagg gctggcggcc agccattcgc cggccggcaa agctctcaaa gaatgacctg
     5941 agacccgttt ctcctccggt ctgagggaga gtcctgggtt tcatttctgg cctcaggcgg
     6001 cctgtctttc cagctcggat ttgagggcga actcggcggg cgtgatgttg ccgagggccg
     6061 agtggggtct gtggcggttg taatcctcct tccatgaggt gatggcgctg cgggcctcgg
     6121 tgagcgtcga gaacagcgtg tcgttgagac actcgtcccg gaagcgtccg ttgaaactct
     6181 cgacgaaggc gttctgcgtc ggcttgccgg gggcgatgta gtgccactcg acgccggtct
     6241 cctggcacca gcgcaggatc gccatcgagg tgaactcggt gccgttgtcg gacacgatcg
     6301 tccccggccg gccgcgcagc cgaatgacgc catccagttc ccgagccacg cgcagacctg
     6361 aaagcgaagt gtcggcgacg agcgccaggc attcgcgcgt ataatcgtcg acgatggcga
     6421 gcaccctgaa gcgacgaccg tccgagatcg tgtcggacag aaaatcgagg ctccagcgcg
     6481 cattggcccg atcaggcacg agcatgggcc tgcgcgtgcc cagtgcccgt ttgcggccgc
     6541 cacgccgtcg cacctgcagc ttctcttcgc gatagagccg tcgcagcttc ttctggttca
     6601 tcacgatgcc ctggcggtcc agcatgatgt ggatcctgcg atagccgaac cgccggcgct
     6661 ccgccgccac gaccttcatc gcctcgcgca acagggcatc gtccggccgg gtgctcgtgt
     6721 agcgcaccgt cgagcggtcg atcgtcaagg ccaggcacgc ccgacgctgg ctcaccgcat
     6781 gcaccgtgca ggcatgagcc accgccttcc gcttcgcatc gggcgtcacc atttttttgc
     6841 ggcgacatcc ttcaggatcg cgttgtcgag catctgttcg gccagcagct tcttgaggcg
     6901 ggcgttctca tcctcgaggg tcttcagccg acgcgcatcc gacacgtcca tcccgccata
     6961 cttcgccttg aacttgtaga aggtcgccga ggagatgccg tgcttgcggc agacatccgc
     7021 cgtcgcagct cc
//
LOCUS       DATMSU010000064         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_133926,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000064 DATMSU010000000
VERSION     DATMSU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133926"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VN034_01610"
     CDS             complement(<1..524)
                     /locus_tag="VN034_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59321.1"
                     /translation="MPGLRIREDGWTAARTRTFLAVLAQSGCVSDAARVAGMSRKSVN
                     DARRRFEEFDRACSTALARALRGLQAIAYERAVVGREMVVIRDGKEVERRIMPSDAML
                     GLLLKRGELGEGGAAGAQYQRYDGDAAISAEEHLAGWRFDGKGIKYFHPGSAIEKLVG
                     KLERIRAADAAEEV"
     regulatory      814..1032
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1150..>2387
                     /locus_tag="VN034_01615"
     CDS             1150..>2387
                     /locus_tag="VN034_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HWV59322.1"
                     /translation="MTVSVATLGFPRIGLRRELKFALENYWAGKSSLAELQTAATGLR
                     AVGWARQKALGADILPSNDFSLYDHVLDTSALVGAVPSRYDWSGATVDPDLYFAMARG
                     AQQPAACGHAHGGDTTAMEMTKWFDTNYHFLVPELTAGQSFRIASTKIFDEYEEAKAL
                     GYATRPVLLGPVSYLMLAKGKAVEPLALLPQLLDTYAEILARLAALGADWVQIDEPCL
                     VLDLTDDQRTAFERAYASLATRGPKLMLTTYFGGLGDNLDLAAELPVHGLHLDLVRAP
                     DQLEPVLTRLQPHVLLSLGVIDGRNIWRANLPDLYWRLKELAAKRDLILAPCCSLLHV
                     PVDLALETSLDPEIAQWLAFAAQKVEELAALAKALNEGEDAGFAAFSASRIAAVARST
                     SPKIHDPAVARRLAALDESAS"
BASE COUNT          407 a          917 c          663 g          400 t
ORIGIN      
        1 tccacttcct ctgcggcgtc tgccgcgcgg atccgctcga gcttgccgac cagcttctcg
       61 atcgcgctgc ccggatggaa atatttgatc cccttgccgt cgaagcgcca gcccgccaga
      121 tgctcctcgg cgctgatcgc cgcgtcgccg tcatagcgct ggtactgcgc ccccgccgcc
      181 ccgccctcgc cgagttcacc gcgcttcagc agcagcccga gcatcgcgtc cgacggcatg
      241 atccgccgct cgacctcctt gccgtcccgg atcaccacca tctcgcgccc caccaccgcg
      301 cgctcataag cgatcgcctg caacccgcgc agcgcgcgcg ccagcgcggt cgaacaggcg
      361 cgatcgaatt cctcaaaacg ccgccgcgcg tcgttgaccg acttgcggct catccccgcg
      421 acgcgcgccg catcgctcac gcaccccgac tgcgcgagca ccgccagaaa ggtccgcgtc
      481 cgcgccgcgg tccacccatc ctcgcgaata cgcagcccgg gcatgagggg attttccttg
      541 atcccagcgt cactacccgt cttgctcccc tcccgcttgc gggaggggtt gggggagggc
      601 ctgttccggc cctcaccatc gcccgaaacc aaaaccgatt tcccccgcga accccgcacc
      661 accttacgcc ccataacccc atctcccgaa tcacttggaa tgaatcggaa cgatcttgaa
      721 cctatttgga taaactagga aagaagaaac tcggccgtaa agttcgatat aaagaattct
      781 ttatatgatg attgacaagc tgcccgaaaa gaatatcctg caaattgtcg aggttcttcg
      841 cgtcgcctgc gcggcgcgaa gctaagacgg gaagccggtg cgccccgaaa ggggcaaggc
      901 cggcgctgcc cccgcaactg taagcggcga gcggcggtca acatgtgtca ctggttcggc
      961 aaggaaccgg gaaggcccga ccgcagcatg gacccgcgag ccaggagacc tgcctcgtca
     1021 gatacgtcct ccggcggggt gtccgggctg gccgcatgca tggctgcgcc ccgtcttgcc
     1081 ttttggggtg cggatcaccg tgcactcgtg cccgtctgtc gctcttgccc atcgcaaggt
     1141 gcttgaaaca tgactgtcag cgtcgccacc ctcggtttcc cccgcatcgg cctccgccgc
     1201 gagctcaaat tcgcgctcga aaattactgg gcgggcaaat cctcgctcgc cgaattgcag
     1261 accgccgcca cgggccttcg cgcggtgggc tgggcgcggc agaaggcgct cggcgccgac
     1321 atcctgccct cgaacgactt ctcgctctac gaccatgtcc ttgacacgag cgcgctcgtc
     1381 ggcgcggtcc cgtcgcgcta cgactggagc ggcgcgaccg tcgatcccga cctctatttc
     1441 gcgatggcgc gcggcgcgca gcaacccgcc gcttgcggcc atgcccatgg cggcgacacg
     1501 accgcgatgg aaatgaccaa atggttcgac accaactatc atttcctcgt ccccgagctc
     1561 accgccggtc agagtttccg catcgcgtcg accaagatct tcgacgaata tgaagaagcg
     1621 aaggcgctcg gctatgcgac gcgccccgtc ctcctcggcc ccgtcagcta tctgatgctc
     1681 gcgaagggca aggccgtcga accgctcgcc ctcctgccgc agctcctcga cacctacgcc
     1741 gaaatcctcg cccgcctcgc cgcgctcggc gccgactggg tgcagatcga cgaaccctgc
     1801 ctcgtgctcg acctcaccga tgaccagcgc accgccttcg aacgcgccta tgccagcctc
     1861 gcgacgcgcg gaccaaagct gatgctcacc acttactttg gcggcctcgg cgacaatctc
     1921 gatctcgccg ccgagcttcc cgtccacggc ctgcacctcg acctcgtccg cgcgcccgat
     1981 cagctcgaac ccgtgctcac gaggctccag ccgcacgtcc tcctgtcgct cggcgtgatc
     2041 gacggccgca acatctggcg cgccaacctc cccgacctct actggcggct caaggagctg
     2101 gcggccaagc gcgacctcat cctcgcaccc tgctgttcgc ttctccacgt ccccgtcgac
     2161 ctcgcgctcg aaacctcgct cgaccccgaa atcgcccagt ggctcgcctt cgccgcgcaa
     2221 aaggtcgagg aacttgccgc gctcgccaag gcactgaacg aaggcgagga cgcgggcttc
     2281 gccgccttct cggcgtcgcg catcgccgcg gtcgcgcggt cgacctcgcc caagatccac
     2341 gaccccgccg tcgccaggcg ccttgccgcg ctcgacgaaa gcgcatc
//
LOCUS       DATMSU010000065         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_133955,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000065 DATMSU010000000
VERSION     DATMSU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133955"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..719)
                     /locus_tag="VN034_01620"
     CDS             complement(123..719)
                     /locus_tag="VN034_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWV59323.1"
                     /translation="MSHILRIDASARQSGSTTRRLADQLEARLVEQGYGAAVTRRDLN
                     LTPPAFLTEHWVGANFTDEAERSDEQKALLAGSEELIAEIEAADTIVITAPIYNFAIP
                     ATLKAWIDQVTRARRTFRYTEAGPEGLLKGKRAYIVFASGGVPLGSQVDFASGYLRHI
                     LGFIGITDVHVIAADGHQMDGDAIARATTAIDGLKQAA"
     gene            complement(793..1488)
                     /locus_tag="VN034_01625"
     CDS             complement(793..1488)
                     /locus_tag="VN034_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWV59324.1"
                     /translation="MIDIRKFDTLGHADHGWLDARHHFSFASYHDPKRMGWGALRVWN
                     DDAIAAQSGFPPHPHRDMEIITYVRTGAISHRDSMGNAGRTAAGDVQVMSAGTGVTHS
                     EFNLEDEETTLFQIWIMPDREGGNPGWGARQFPKNDRSGQFVTLASGIEGDDALPIRA
                     NARVAAATVNAGESVSYDLEAGRHAYLVAAKGRIRVNDEEADPRDGIALRDVGTIIVE
                     ALDDAELVLVDSL"
BASE COUNT          266 a          483 c          599 g          311 t
ORIGIN      
        1 atagctcgaa taataaagca ccagaacctt ggtcatcatg attgtccttt cgttgatcga
       61 aaagcgggag gggtgggccg ccgccggggg gagggggaag gagggcggcg gcccgagggg
      121 tgtcaggcgg cctgtttcag cccgtcgatc gcggtcgtcg cgcgggcgat cgcatcgccg
      181 tccatctggt gaccgtcggc ggcgatgaca tgcacgtcgg tgatgccgat gaagccgaga
      241 atatgccgca gatagccgct cgcgaaatcg acctggctgc ccagcggcac gccgcctgaa
      301 gcgaagacga tataggcgcg cttgcccttg agcagccctt caggaccggc ctcggtgtag
      361 cggaaggtgc ggcgcgcgcg cgttacctgg tcgatccagg ccttcagcgt cgcggggatc
      421 gcgaaattat agatgggcgc ggtgatgacg atcgtgtcgg ccgcctcgat ctcggcgatc
      481 agctcttcgg acccggcgag cagtgccttc tgctcgtcgc tgcgttcggc ttcgtcggtg
      541 aagttggcgc cgacccaatg ttcggtgagg aaggcgggcg gggtgaggtt gaggtcgcgg
      601 cgcgtgactg ccgcgccata gccttgttcg acgaggcggg cctcgagctg gtcggcgagg
      661 cggcgggtgg tcgagcccga ctggcgggcg ctggcgtcga tgcggagaat atggctcatg
      721 gaaatcgcct ttcgaaaaag agaggggtgg gccgccgccg agggaggggg agggcggcgg
      781 cccgggggct cgtcagaggc tgtcgacgag cacgagttcg gcgtcgtcga gcgcctcgac
      841 tatgatcgtg ccgacatcgc gaagcgcgat gccgtcgcgc gggtcggctt cctcgtcgtt
      901 gacgcggatg cggcccttgg cggcgaccag ataggcgtgg cgcccggctt cgaggtcata
      961 ggagacgctc tcaccagcgt tcaccgttgc cgcggcgacg cgggcgttgg cccggatcgg
     1021 caaggcatcg tcaccctcga tcccgcttgc cagcgtcacg aactggcccg agcggtcatt
     1081 cttgggaaac tggcgtgcgc cccagcccgg attgccgccc tcgcgatcgg gcatgatcca
     1141 gatctggaac agcgtcgttt cctcatcctc gaggttgaac tcgctgtgcg tcacgcccgt
     1201 cccggcgctc atcacctgaa catcgcccgc cgcggtgcgg ccggcattgc ccatggagtc
     1261 gcggtggctg atcgcgccgg tgcgaacata ggtgatgatt tccatgtcgc ggtgcgggtg
     1321 cggggggaaa cccgactgcg ccgcgatggc gtcgtcgttc cagacgcgca gcgcgcccca
     1381 gcccatccgc ttcggatcat ggtagctcgc gaaggagaaa tggtgacggg cgtcgagcca
     1441 gccatggtcg gcgtggccca gcgtgtcgaa tttgcgaata tcgatcattg gccttgtcct
     1501 ttgcttgccg cccgtcggct tgggtgcccg gcggctcatc tgttgaggac aagataggcg
     1561 cttggatcgt ttctaaaata gcgtagatgg aaaaccatcg tttccgtttt taagttgata
     1621 ggataggaaa gcccctcccc ttcaggggag gggttgggg
//
LOCUS       DATMSU010000066         5494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_105699,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000066 DATMSU010000000
VERSION     DATMSU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105699"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VN034_01630"
     CDS             complement(<1..1161)
                     /locus_tag="VN034_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV59325.1"
                     /translation="MSELDAFRTEVREWLEANCPPEMREPVRDEGDVCWGGRNWVFKN
                     DAQKQWLEACVAKGYTVPDWPKPYGGAGLTPEQTKILKQEMKRIHARSPLDSFGIWML
                     GPALLQFGTEEQKVQYLNPIARGEIRWCQGYSEPGSGSDLVSLQTFGEDKGDHWVVNG
                     SKIWTSYADKADWIFCLVRTDKANKYQGITFMLFDMASKGVTTKPILLISGNSPFCET
                     FFDDVEVPKTQFIGEINRGWDVAKYLLGHEREMISGGGAGGDMVSIGGAFAKAFGKNA
                     AGELDDPILRAEMAAFDVDVFAYRAMGERFMDMWKTGRAHPASSNMMKYVGTELNKRR
                     HELVMSGGGSEALEWDSEETKGGARARGWLRTKANSIEGGTSEVMLNVIAKRI"
     gene            1335..2192
                     /locus_tag="VN034_01635"
     CDS             1335..2192
                     /locus_tag="VN034_01635"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59326.1"
                     /translation="MRFAGKIAVVTGAASGIGKATVLKLAGEGAHVFAADIDEAGGQA
                     LAEQSNGRISFVRCDVTRPADIEALMNEAATKAGGIDIVFNNAAAGGDRAPIDEISPE
                     GWDRTMDLVLKSVAMGIRYASPHMKHRKGASIVNTASVAALGAGYSPIAYAVAKAGVL
                     HLSKVAATDLAQYGIRVNAICPGFINTNIFTASLDVPGELKTQANEVIAEMSANAQPV
                     ARGGQPEDIANAVAYLASEESSFMTGTHLLVDGGLTIGQRHAWDKETPGIFDALLAME
                     EAAKAGAPA"
     gene            2189..3652
                     /locus_tag="VN034_01640"
     CDS             2189..3652
                     /locus_tag="VN034_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59327.1"
                     /translation="MSAASIAAPPRPERLPVWLKLVHGLGSIAYGVKENGFSTFLLLF
                     YNQVIGLDAGIVGIAIMVALIFDAFIDPIIGELTDRTRSRWGRRLPWLYVAPFPLAFA
                     WMALWSPPEMSDAATVAWLIGFAIVVRALVSMCEVPSVAIVPELTADYDERTAVMRYR
                     FLFGWGGGLVILVLAYGVFFGGAKGLVDPAGYFPYALTGALVMLGAVVISAAGQHKRI
                     AVSNLADRKPAMSLSHILRDMRDTLSNRAFLWLVFAALFGFVNQGITFSMNNYLLSFF
                     WQFTQGEMVAYVFLLFGTMIAAFLLVAPLSAKLGKRDGAILAGAISLIVNSGIYFAWI
                     QGFFPGLPGKPSVAWMFGLVFVSNTFSIILMILSSSMMADVVEASQSETGRRSEGLFF
                     AGYFFMQKCATGIGIFAAGMILSFAAFPAGAEPGQVSNAVLGDLALGYMLSVLVIGIV
                     GLIVMRRFPISRADHEARLALLDDAARAEPDASGAHP"
     gene            3763..5052
                     /locus_tag="VN034_01645"
     CDS             3763..5052
                     /locus_tag="VN034_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV59328.1"
                     /translation="MDFDLTDRQVYWRDRVRDFIERKVRPAVPTYYEQDKAGDRWKVI
                     QIVEDLKAEAKEAGIWNLFMPPKSGAHHHVDETFEFEGPGLSNLEYALCAEEMGRVGF
                     ASEVFNCSAPDTGNMEVFHRYGTRAQKDKYLGRLMNGQIRSAFLMTEPRVASSDATNI
                     ETRIERDGDDYVINGTKWWSSGAGDPRCEVAIVMGKTDFAAKRHAQQSMVLMPLNAPG
                     VNILRHLPVFGYDDAPHGHMEIELKDVRVNAEEAMLLGEGRGFEIAQGRLGPGRIHHC
                     MRTIGVAEEAIAKMAKRLQSRVAFGKKVAEYSIWEHRLARARIDIEMTRLLCLKAADM
                     MDKVGNKSAAAEIAMIKVQAPNMALKIIDDAIQAHGGGGVSEDFGLAKAYAHQRTLRL
                     ADGPDEVHERAIARIEFAKHSEASEPGFSSGDVGVSR"
     gene            5162..>5494
                     /locus_tag="VN034_01650"
     CDS             5162..>5494
                     /locus_tag="VN034_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWV59329.1"
                     /translation="MTKAAILIEPGTPLSIETVQIADCGPHEVRIRTAACGLCHSDLH
                     FIDGAYPHPLPAIPGHEAAGIVEAVGSEVRTVKVGDAVVTCLSAFCGHCEFCVSGRMS
                     LCMGGDTRR"
BASE COUNT          936 a         1744 c         1787 g         1027 t
ORIGIN      
        1 tatccgcttg gcgatgacgt tgagcatgac ttcgctcgtc ccgccttcga tcgaattggc
       61 cttggtgcgc agccagccgc gcgcgcgcgc gccgcccttg gtctcttcgc tgtcccattc
      121 gagcgcttcg ctgccgccgc ccgacatgac gagttcgtgg cgccgcttgt tgagctcggt
      181 gccgacatat ttcatcatgt tcgagctggc gggatgcgcg cgcccggtct tccacatgtc
      241 catgaagcgc tcgcccatcg cgcggtacgc aaagacatcg acgtcgaacg ccgccatttc
      301 ggcgcgcagg atcgggtcgt cgagctcgcc cgcggcattc ttgccgaacg ccttggcgaa
      361 cgcgccgccg atgctgacca tgtcgccgcc cgcgccgccg ccggagatca tctcgcgttc
      421 gtggccgagc agatatttgg cgacgtccca gccgcggttg atttcgccga tgaactgcgt
      481 cttgggcact tcgacatcgt cgaaaaaggt ttcgcagaag ggcgagttac cgctgatgag
      541 caggatcggc ttggtcgtga cgcccttgct cgccatgtcg aagagcatga aggtgatgcc
      601 ctgatattta ttcgccttgt cggtgcggac gaggcagaag atccagtcgg ccttgtcggc
      661 gtagctcgtc cagatcttcg acccgttgac gacccaatga tcgcccttgt cttcgccgaa
      721 ggtctgcaag ctgacgaggt cgctgcccga gccgggttcc gaatagccct ggcaccagcg
      781 aatttcgccg cgcgcgatcg ggttcagata ttggaccttc tgttcctcgg tgccgaattg
      841 cagcagcgcg gggccgagca tccagatgcc gaagctgtcg agcggcgagc gcgcgtggat
      901 gcgcttcatt tcctgtttga ggatcttggt ctgttcgggg gtgagccccg cgccgccata
      961 gggcttgggc cagtcgggca cggtgtagcc cttcgcgacg caggcctcga gccattgctt
     1021 ctgcgcgtca ttcttgaaca cccagttacg cccgccccag cagacgtcgc cctcgtcgcg
     1081 cacgggctcg cgcatttcgg gcggacaatt cgcctccagc cattcgcgca cctcggtgcg
     1141 gaacgcgtcc agttcgctca tccttttggc tcctcttctg cgcccaaatc tacgtccgct
     1201 tcgctgcttt acgcaagcgt caacttgggc ggacgcccct gccgttacgg cgccgtagcg
     1261 tcggattgac cgccgttgac gcgtcccctc tggcgcctaa gctaagtggc aaaagaaaac
     1321 gggagaatgg gtcaatgcga ttcgcaggca agatcgccgt cgtcaccggc gcggcgtcgg
     1381 gcatcggcaa agccacggtg ctgaaactgg cgggcgaagg cgcgcatgtc ttcgccgccg
     1441 acatcgacga agcgggcgga caagcgctcg ccgagcagtc gaacggcagg atcagcttcg
     1501 tccgctgcga cgtcacccgg cccgccgaca tcgaggcgct gatgaatgaa gccgccacga
     1561 aagccggggg catcgacatc gtcttcaaca acgccgccgc gggcggcgac cgcgcgccga
     1621 tcgacgagat cagccccgag ggctgggacc gcacgatgga cctcgtcctc aaatcggtcg
     1681 cgatgggtat tcgttatgcc tcgccgcata tgaagcatcg caagggcgcg tcgatcgtca
     1741 acaccgcgag cgtcgcggcg ctcggcgcgg gctattcgcc gatcgcttat gcggtcgcta
     1801 aggcgggggt gctccacctc tccaaggtcg cggccactga tctggcgcaa tatggcatcc
     1861 gcgtgaacgc gatctgtccc ggtttcatca acaccaatat cttcaccgcc tcgctcgacg
     1921 tgccgggcga actcaagacg caggccaatg aagtgatcgc cgagatgagc gcgaacgcgc
     1981 agcccgtcgc gcgcggcggt cagcccgagg atatcgccaa cgcggtcgcc tatctcgcga
     2041 gcgaggagtc gagcttcatg acaggcaccc acctgctcgt cgacggcggg ctgacgatcg
     2101 gccagcgcca cgcgtgggac aaggaaacgc cgggcatatt cgacgccctg ctcgcgatgg
     2161 aggaagcggc gaaggccgga gcccccgcgt gagcgcggca agcatcgcgg cgcccccgcg
     2221 ccccgaacgg ctgccggtct ggctgaagct ggtgcatggg ctcggcagca tcgcttacgg
     2281 cgtcaaggag aacggctttt cgaccttcct cctgctgttc tacaatcagg tcattggcct
     2341 cgacgcgggt atcgtcggca ttgcgatcat ggtcgcgctg atcttcgatg cgttcatcga
     2401 cccgatcatc ggcgagctga ccgaccgcac gcgcagtcgc tggggccgcc gcctgccgtg
     2461 gctgtatgtc gcccccttcc cgctcgcatt cgcgtggatg gcgctgtgga gcccgcccga
     2521 aatgagcgac gcggcgaccg tcgcctggct gatcggcttt gcgatcgtcg tgcgcgcgct
     2581 ggtgtcgatg tgcgaagtgc cgtcggtcgc gatcgttccc gaactcaccg ccgactatga
     2641 cgagcgcacc gcggtgatgc gctaccgctt cctgttcggc tggggcggcg ggctcgtcat
     2701 cctcgtcctc gcctatggcg tgtttttcgg cggcgccaag gggctggtcg atccggcggg
     2761 ctatttcccc tatgcgctga ccggcgcgct cgtcatgctc ggcgcggtcg tcatctcggc
     2821 ggcgggtcag cacaaaagga tcgcggtgtc gaacctcgcc gaccgcaagc ccgcaatgag
     2881 cctgtcgcat atcctgcgcg acatgcgcga cacgctgtcg aaccgggctt ttctgtggct
     2941 ggtgttcgcg gcgcttttcg gcttcgtcaa tcagggcatc accttttcga tgaacaatta
     3001 tctgctgagc tttttctggc agttcacgca gggcgagatg gtcgcctatg tctttctgtt
     3061 gttcgggacg atgatcgcgg ccttcctgct cgtcgcgccg ctctcggcaa agctcggcaa
     3121 gcgcgacggc gcgattctgg ccggggcgat ctcactgatc gtcaacagcg gcatctattt
     3181 cgcgtggatc caggggtttt tccccggcct gccgggcaag ccgagcgtcg catggatgtt
     3241 cgggctggtg tttgtcagca acactttctc gatcatcctg atgatcctct cctcgtcgat
     3301 gatggccgac gtcgtcgaag cctcgcaaag cgaaacgggc cgccggtcgg aggggctgtt
     3361 tttcgcgggc tatttcttca tgcagaaatg cgcgacgggc atcggcatct tcgccgcggg
     3421 gatgatcctg tccttcgccg cctttccggc cggagcggag ccgggacagg tcagcaacgc
     3481 ggtgctcggc gatctggcgc tcggctacat gctttcggtg ctggtgatcg ggatcgtcgg
     3541 gctgatcgtg atgcggcgct tcccgatctc gcgcgccgat catgaagcgc ggctggcgct
     3601 gctcgatgac gccgcgcgcg ccgagcccga cgcgagcgga gcgcacccct gagcgaaaaa
     3661 tgcgcgccat gaccaaatgg ggtaattttc ggaccgcagg acttggcaag gccggcgaat
     3721 cggtccaagg gtttacgtga acgtaaaggg aaggatgcgg agatggattt cgatctcacc
     3781 gaccggcagg tctattggcg cgaccgcgtg cgcgacttca tcgagcgcaa ggtccgtccc
     3841 gcggtcccga cctattatga gcaggacaag gcgggcgacc gctggaaggt catccagatc
     3901 gtcgaggatc tgaaggccga ggcgaaggaa gccggcatct ggaacctgtt catgccgccc
     3961 aagtcgggcg cgcatcatca tgtcgacgag acgttcgagt tcgaaggccc cggcctctcc
     4021 aacctcgaat atgcgctgtg cgccgaggaa atgggccgcg tcggttttgc gagcgaggtg
     4081 ttcaactgct cggcgcccga caccggcaat atggaagtgt tccaccgtta cgggacgcgc
     4141 gcgcagaagg ataaatatct cggccggttg atgaacgggc agatccgctc ggcctttctg
     4201 atgaccgagc cgcgcgtcgc ctcgtccgac gcgacgaata tcgaaacgcg catcgagcgc
     4261 gacggcgatg actatgtgat caacggcacc aaatggtggt cgtcgggcgc gggcgacccg
     4321 cgctgcgagg tcgcgatcgt gatgggcaag accgactttg ccgcgaagcg ccacgcgcag
     4381 cagtcgatgg tgctgatgcc gctgaatgcg cccggggtga acatcctccg ccacctgccc
     4441 gtattcggtt atgacgatgc gccgcacggc catatggaaa tcgagctgaa ggacgtccgc
     4501 gtgaacgccg aggaagcgat gctgctcggc gagggccgcg gattcgagat cgcgcagggc
     4561 cgcctcgggc cgggacgcat ccatcactgc atgcgcacga tcggtgtcgc cgaggaagcg
     4621 atcgcgaaga tggcgaagcg gctgcaatcg cgcgtcgcgt tcggcaagaa ggtcgccgaa
     4681 tacagcatct gggaacatcg cctcgcccgc gcgcggatcg acatcgaaat gacgcgcctc
     4741 ctctgcctca aggcggccga catgatggac aaggtcggca acaagtcggc cgcggccgaa
     4801 atcgcgatga tcaaggtgca ggcaccgaac atggcgctca agatcatcga cgatgcgatc
     4861 caggcgcatg gcggcggcgg cgtgtccgag gatttcgggc tggccaaagc ctatgcgcat
     4921 cagcgcacgc tccgcctcgc cgatggtccc gacgaagtgc acgaacgcgc gatcgcccgc
     4981 atcgaattcg ccaagcatag cgaagcgagc gagccgggct tctcgtcggg cgatgtcggg
     5041 gtatcacgct aagagataat ccgttcgtgc tgagcttgtc gaagcaccgt ccttcttgct
     5101 tcgaagagaa gaacggccct tcgacaggct cagggcaaac ggtcagagag ggtagtttag
     5161 aatgaccaaa gccgcgattc tgatcgaacc gggtacgccg ctgtcgatcg agacggtgca
     5221 gattgccgac tgcggcccgc acgaggtgcg catccgcacc gccgcgtgcg ggctgtgcca
     5281 ttcggacctg catttcatcg acggcgccta tccgcatccg ctgcccgcga tcccgggcca
     5341 cgaagccgcg ggcatcgtcg aggcggtcgg cagcgaagtg cgcacggtga aggtcggcga
     5401 cgcggtcgtc acctgcctca gcgccttctg cggccactgc gaattctgtg tgtcgggccg
     5461 catgtcgctg tgcatgggcg gcgacacgcg gcgg
//
LOCUS       DATMSU010000067         9152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181516,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000067 DATMSU010000000
VERSION     DATMSU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181516"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VN034_01655"
     CDS             complement(<1..1116)
                     /locus_tag="VN034_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV59330.1"
                     /translation="MRHVAIVGSGPAGYYTAETLQKADEIAVDVIDRLPVPYGLIRTG
                     VAPDHQSIKAVSRRYEGTALTDNVRFVGHVSVGSDVTIDELVSLYDAVILATGAPNDR
                     PLDIAGADLPGVIGSAAFVGWYNGHPDFADLDPPLGAPGVAVIGNGNVALDVARILAK
                     TPGEFAGSDIVAHARDILAASAVRHIQILGRRGPHQIAMTPKELGELGHLERASPRVD
                     PADLPDEGDDAMLEPGMRKSVTHLRGFAANPVAKPVTIDFDFFAMPVAIEGDGRVQRV
                     IVEKTVLDADLRSHGTGETYAVDAGLVISCIGYQTPPIPGVPYEHGRGRFASDEGRIL
                     PGLYAVGWARRGPSGTIGTNKPDGARIGEMELEDIGRG"
     gene            1271..1344
                     /locus_tag="VN034_01660"
     tRNA            1271..1344
                     /locus_tag="VN034_01660"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Gln,seq:ctg)
     gene            complement(1490..2416)
                     /locus_tag="VN034_01665"
     CDS             complement(1490..2416)
                     /locus_tag="VN034_01665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009604.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase"
                     /protein_id="HWV59331.1"
                     /translation="MSSLTGKRVLMLHNAKARQGEGALEPVCNQLAAGGFAVTVESFE
                     NLPEIARDITRLHRTADMVVVCGGDGSISSAAPAVIESKLPLGIIPAGTANDLARTLS
                     IPLDFAAAADVIIAGHLRQIDVGMVNGHAFFNVASIGLSSELAQKLDPAIKKRFGRFG
                     YAIAAFGILARARRFRARIVEKGSNTSVRTYQIAIGNGRLYGGGNIVEETAAIDDGTL
                     DLYSLEMRNLWKLALMVRAFRSGTHGAWKEVRTARCVEFDIETRRPMPINTDGEIATA
                     TPAHFRVLPAAITVLAPPDAGAASRSIRLYHR"
     gene            2730..3224
                     /locus_tag="VN034_01670"
     CDS             2730..3224
                     /locus_tag="VN034_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59332.1"
                     /translation="MIEATHVVAAAGEEAPARAILIGGLAGGVLDLGFAFTAWALKGV
                     GPADILRSIASGLLGPAARTGFAPVPLGLLCHFLLSFLFAAAFVLVTMRFAAVGRQSP
                     WLAGPLYGVAVYFVMNRVVLPLSNFAVPPGGMALGFADLASHMFLFGLPIALAAHRWA
                     RVAS"
     gene            3252..6113
                     /locus_tag="VN034_01675"
     CDS             3252..6113
                     /locus_tag="VN034_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59333.1"
                     /translation="MLYKFRPRHMLLSTTLLGSVACIAPAYAQDAPGGETAIEDTAPI
                     VVTGSRIQNPNLEQSSPVQVVGETEIGLRQATNAEELIGDLPGVSPGANSAVNNGGAG
                     FASLNLRGVGTNRNLVLLDGTRLVPSSLLSETDLNIIPVALIERAEIVTGGASSVYGA
                     DAIAGVANFITKRDFAGAEIASTFGITERGDGARYRFDVTVGANFDDGRGNAVLNVGY
                     QKVEPVLQGGRAVSRDTYFLDGSLVGSGTTTPTRINNRIYNPADGALRDYVGSRDAYN
                     FAPDNYFQTPLERYNMFGAAQYEVADGIEIYAKGMFTRSKVQLSLASSGMFGDTWQLP
                     LNNPFLPDAVRSEVCASNAISAADCAAAGAATSPNDPAYREVATVINRRFIEAGPRIR
                     NLTTNQFQVWAGVRGSITDAIDFDVYGLHGESDRKNSNINWGLKSRVQQALRANSATE
                     CADPSNGCVPINLFGDGTDITDEAIAFFNQPAGSTVSTRLSVVSGSISGELGNFLAGE
                     TPIGFALGAEYRKYGAEQVSDVAFGTQDEVLGTGAPSPSFAGTYDVKEIFGELIVPIV
                     EDVPGIYSLTAEAGIRLSDYSNTGTSTTWKAGGTWEPFRGYKLRGIYQHSVRSPNISE
                     LFTPITTGLANLAEDPCQGSSPVGNAALTAICIAQGAPPGTIGNIPAPSSGQINQTAG
                     GNPNLDVETADSYTLGLVVTPPQVPGLAITADYYHIKITDAITLPTPNDVFGPCFEEG
                     DAAACALIRRNPLNGSLNGGGDTPGQIALLTNQGTITTSGVDLRINYGLPTGFGRLNF
                     DLSGNWTEQSKFKASPTSINRECVGQYSSNCGFFAGEITPKYSFNMRATAQIDDFGDI
                     SLLWRWLDGSDYERILDADGIEPDYLHIPSYSYFDLTYRAKVADIFTLTLSVQNLLDK
                     DPPLVSNYVGTTALNSGNTYPTTYDVLGRRYSLTASMRF"
     gene            6335..7459
                     /locus_tag="VN034_01680"
     CDS             6335..7459
                     /locus_tag="VN034_01680"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWV59334.1"
                     /translation="MNQVAAFPSTGVYEVATAGRPGSLERQRRQAILWLTGAFWGLHT
                     LALWIADLLDHHPHILRATAVRLMLMAIGLALCFGIHMILQKLSRRAWRQKAPIVFFA
                     SLVAAELFAWCTYFALVYAKGEPVQLAIDGSAVIRTLVPWTWFFLAWVGLYLAVQYGF
                     EARAEERRSAQLRTMAQAAKLQALHYQINPHFLFNSFNAVSALILDGRADDANAMVER
                     LAGFFRVNLATNPDVDISLAEEVALQATYLEIEQTRYPDLEVRIDLPPALGRAAVPAF
                     LLQPLVENAVKHGAGTVGAGDAWIAISARQEQGRLEVVVENSCSPSRGGAGICGTHTG
                     LRNVRDRLSNRFGADAKLSIERLPASRFRATLELPLGYAR"
     gene            7456..8250
                     /locus_tag="VN034_01685"
     CDS             7456..8250
                     /locus_tag="VN034_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWV59335.1"
                     /translation="MTMRVLTVDDEPLALRRLELILAKLPEIEHVGSASGCREAIAKI
                     AELKPDIVLLDIKMRDGTGFDVLSRLPEDGVPGVIFTTAFDHFAVRAFEAHAIDYILK
                     PIEISRLRAAIDRSRDRLAAADAHEQIAQMRAVIDDLRAGYHEQGVRQRDDELWLRGT
                     AGALTCIQRADIDMITAEDDYVRLHTASASYLLRGSIRAAQADLDLPEEEFVRVHRRA
                     LLRRSAIASVRRSAGKGVRVILRNGTDVGAGRVYAKKLLRSLPAVG"
     gene            complement(8295..9029)
                     /locus_tag="VN034_01690"
     CDS             complement(8295..9029)
                     /locus_tag="VN034_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59336.1"
                     /translation="MSMSEVDKIRITAASAIERSRERRRPIGTKSVALRRQHLYKKLG
                     RMLIAGGIVLIGAAVFGALIQPLGFIGLLALFALLIGVAVLFGKWPPFPEPRQTDLPK
                     ADLKQLAGRTEIWLEAQRPALPAPARQLVDGIGVQLDLLAPQLQTLDASSPAAANIRK
                     LVGEDLPELVAGYQRIPAGLRKEAHAGRTPDETLVGGLKMLEGEIGNAARSLAAGELD
                     KLATRERYLQLKYQGIDGEEPPASQA"
BASE COUNT         1472 a         3001 c         3022 g         1657 t
ORIGIN      
        1 gccgcggccg atatcctcca gctccatttc gccgatgcgc gcgccgtcgg gcttgttcgt
       61 gccgatcgtg cccgatggcc cgcgccgcgc ccagccgacc gcgtagagac cgggcaggat
      121 gcgcccttcg tcgctcgcga agcggccgcg cccatgttcg taggggacgc cggggatcgg
      181 cggggtctgg tatccgatgc aactgatcac gaggccggcg tcgacggcat aggtttcgcc
      241 cgtgccatgg ctgcggaggt cggcgtcgag gacggtcttc tcgacgatga cgcgctggac
      301 tcttccgtcg ccttcgatcg ccacgggcat cgcgaagaag tcgaaatcga tcgtcaccgg
      361 cttggcgacc gggttggcgg cgaagccgcg gaggtgcgtc accgacttgc gcatcccggg
      421 ctcgagcatc gcgtcgtcgc cctcgtcggg caggtcggcg gggtcgacgc gggggctcgc
      481 gcgctcgaga tgaccgagtt cgccaagctc cttcggcgtc atcgcgatct ggtgcgggcc
      541 gcggcggccg aggatctgga tatggcgcac cgcgctcgcc gccaatatat cgcgcgcgtg
      601 cgcgacgatg tcgctgccgg cgaattcacc gggggtcttg gcgaggatgc gcgcgacgtc
      661 gagcgcgaca ttgccattgc cgatcacggc gacgccgggg gcgcctagcg gcggatccaa
      721 gtcggcgaag tcgggatggc cgttgtacca gccgacgaag gcggcgctgc cgatcacgcc
      781 ggggaggtcg gcgccggcga tgtcgagcgg gcggtcgttc ggcgcgccgg tcgcgaggat
      841 cacggcgtcg tagaggctga caagctcgtc gatcgtcacg tcgctgccga ccgagacatg
      901 cccgacgaag cggacattgt cggtgagcgc ggtgccctcg tagcggcgcg acaccgcctt
      961 gatcgattga tggtcggggg cgacgccggt gcggatcaga ccatagggca cgggcagccg
     1021 gtcgatgacg tcgaccgcga tctcgtccgc tttctgtagt gtttcggcgg tgtaatagcc
     1081 cgcgggaccc gatccgacga tcgcgacatg acgcatcaac ccactccttc gggcatccgc
     1141 gcgggcgcgc ggcccctctt ttgttatggg gtaatgctag cggcgaaatg cgtcagggca
     1201 agcgcgcttg tacgggctgc cgctccggcc ttgacaggcg gccttcgcaa tcatattgcg
     1261 cgcgcctcat tgccccgtcg tctaatggta agactacgga ttctgattcc gtctattgag
     1321 gttcgaatcc tcacggggca tccattcctt cggtatttcg gcgcaaacgc acgaagagtc
     1381 ctctggcgct gccggggcag gaatcgcact tgtggcgtcg ggaagccttc cgggccgcgg
     1441 caagcaagct tggcgtccgg tttcgccggg gccgagtcga accgttatct cacctgtgat
     1501 acaggcggat ggagcgagaa gccgctccgg cgtcgggcgg cgcgagaacc gttatggctg
     1561 caggcaatac ccgaaaatgc gccggggtcg cggttgcgat ctcgccatcg gtgttgatcg
     1621 gcatcgggcg ccgtgtttct atgtcgaact ccacgcatcg cgcggtccgc acttccttcc
     1681 acgccccgtg cgtgccggag cggaaagcgc gcaccatcag ggcgagtttc cagagattgc
     1741 gcatttcgag gctgtaaaga tcgagcgtgc cgtcatcgat ggcggccgtt tcctcaacaa
     1801 tgttgccgcc gccgtacagg cggccgttcc cgatcgcgat ctgataggtt ctgacgctcg
     1861 tattgctgcc cttctcgacg attctcgcgc ggaaacgccg ggcccttgcc aggatcccga
     1921 atgcggcgat ggcgtaaccg aaccggccga agcgcttctt gatcgccgga tcgagtttct
     1981 gcgccagttc gctgctcagc ccgatactcg ccacgttgaa gaaggcgtga ccattgacca
     2041 tgccgacgtc gatctgccga aggtggccag cgataatgac gtcggcggcg gcggcgaaat
     2101 cgagagggat ggaaagcgtc cgcgcgaggt cgtttgccgt tcccgccgga atgatgccga
     2161 gcggcagctt gctttcaatg accgccggcg ccgccgagga gatcgatcca tccccgccgc
     2221 aaaccacgac catgtccgcc gtgcgatgca agcgcgtgat gtcgcgcgcg atctcgggaa
     2281 gattttcgaa cgactcgacg gtgacggcaa agccgcctgc cgcgagctgg ttgcataccg
     2341 gctccaacgc gccttcgcct tgccgcgcct tcgcattgtg gagcatgagg acgcgcttgc
     2401 cggtcagcga cgacattgag atgatcctgc cggaagaaag atgggccagc gaccccgatc
     2461 cgaatgaaag accgattctc gcctcgctcc gcgatacagg tctaagacct gttggagaag
     2521 gggtgagatt gccgagcggg gcgctgccaa ctccggcagc gccctcttgg gcgcatcgac
     2581 gtgcgcgcac gggccgttcg ccccgcagga tgtgacgttc gcctgaccgg cttgcgttgg
     2641 gctgcatcgg agcgccgacc ggccgccatt cgctccaacc ggtgatccgc ccgcgctagc
     2701 gccccctttc ggaagcaaag gggacgaaga tgatcgaggc tacccatgtc gtcgcggcag
     2761 cgggcgaaga ggcgcctgcc agggcgatcc tgatcggcgg gctggcgggc ggtgttctcg
     2821 atctggggtt cgcctttacc gcctgggcgc tgaagggcgt cggtccggcc gacatattgc
     2881 ggagcatcgc gtcggggctg ttgggtccgg cggcgcggac cggctttgcc ccggtgcccc
     2941 tggggctgct gtgccatttc ctgctctcgt tcctgttcgc ggcggcgttc gttctcgtca
     3001 ccatgcggtt cgcggcggtg gggcggcaat ctccctggct tgccgggccg ctctacggcg
     3061 tcgccgtcta tttcgtgatg aaccgcgtcg tgctgccgct ctcgaacttc gccgttccgc
     3121 ccggcggcat ggcgctcggc ttcgcggacc tcgcctcgca catgttcctc ttcggcctgc
     3181 cgatcgcgct cgcggcgcat cgctgggcgc gggtcgcatc ataaatcgaa taataaacgg
     3241 ggagtttcaa tatgttgtat aagtttcgac cgcgtcatat gctgctttcg acgacattgc
     3301 tcggaagcgt cgcctgcatt gcccccgctt atgcacagga cgcgccgggc ggcgagacgg
     3361 cgatcgagga tacggcgccg atcgtcgtca ccggctcgcg catccagaac cccaacctcg
     3421 agcaatcgag ccccgtgcag gtcgtgggcg aaaccgaaat cgggcttcgt caggcgacca
     3481 atgccgagga gctgatcggc gatctgcccg gcgtgtcgcc gggtgccaac agcgcggtca
     3541 acaacggcgg cgcgggtttc gcttcgctca acctgcgagg cgtcggcacc aaccgcaatc
     3601 tcgtgctgct cgacgggacg cggctcgtgc cgtcgtcgct gctcagcgag accgacctca
     3661 acatcattcc cgtcgcgctg atcgagcgcg ccgagatcgt gacgggcggc gcttcgtcgg
     3721 tctatggcgc cgacgcgatc gccggcgtcg cgaacttcat caccaagcgc gacttcgcgg
     3781 gggcggagat cgcgagcacc tttggcatca ccgagcgcgg cgacggtgcg cgctatcgct
     3841 tcgacgtgac ggtcggcgcc aatttcgacg acggccgcgg caatgcggtg ctgaatgtcg
     3901 gctatcagaa ggtcgagccg gtgctgcagg gcggccgcgc cgtttcgcgc gatacctatt
     3961 tcctcgacgg cagcctggtc ggttcgggga cgacgacccc gacgcgcatc aacaaccgca
     4021 tctacaaccc cgctgatggt gcgctccgcg actatgtcgg gtcgcgcgac gcctataatt
     4081 tcgcgcccga caattatttc cagaccccgc tcgaacgcta taatatgttc ggcgcggcgc
     4141 aatatgaggt cgccgacggc atcgaaatct atgccaaggg catgttcacc cggtcgaagg
     4201 tgcagctcag cctcgcctcg tcggggatgt tcggcgacac atggcagttg ccgctcaaca
     4261 atccattcct gcccgatgcc gtgcgctcgg aggtttgcgc gtcgaacgcg atctcggccg
     4321 ccgactgcgc cgccgccggc gccgcgacca gcccgaacga tcccgcttac cgcgaggtcg
     4381 cgacggtcat caaccgccgc ttcatcgagg ccgggccgcg catccgcaac ctgacgacca
     4441 accagttcca ggtctgggcg ggcgttcgcg ggtcgatcac cgatgcgatc gatttcgacg
     4501 tctatggcct gcatggcgaa tcggaccgca agaacagcaa tatcaattgg ggcctgaaat
     4561 cgcgcgtgca gcaggcgctg cgcgccaaca gcgcgaccga atgcgccgat ccgtcgaacg
     4621 ggtgcgtgcc gatcaacctc ttcggcgatg gcaccgatat tacggacgag gcgatcgcct
     4681 tcttcaacca gccggcggga tcgacggtga gcacgcgcct gtcggtcgtc agcggcagca
     4741 tctcgggcga gctgggcaat ttcctggccg gggaaacgcc gatcggcttc gcgctgggcg
     4801 ccgaatatcg caaatatggc gccgagcagg tctcggacgt cgccttcggg acgcaggacg
     4861 aagtgctggg caccggggcg ccatcgccgt ccttcgcggg cacctatgac gtcaaggaaa
     4921 tcttcggcga actgatcgtg ccgatcgtcg aggatgtacc cggcatctac agcctgaccg
     4981 ccgaagcggg catccgcctg tcggactatt cgaacacggg cacgagcacg acgtggaagg
     5041 cggggggcac ctgggaaccg ttccgcggct acaagctgcg cggcatctat cagcattcgg
     5101 tgcgctcgcc gaatatctcg gagcttttca ccccgatcac cacggggctt gcgaaccttg
     5161 ccgaagatcc gtgccagggt tcgagtcccg tcggcaacgc cgcgctcacc gcgatctgca
     5221 tcgcgcaagg cgcccctccc ggcacgatcg ggaatatccc ggcgccttcg tcgggtcaga
     5281 tcaaccagac ggcgggcggc aacccgaatc tcgatgtcga gaccgccgat tcctacacgc
     5341 tgggactggt cgtgacgccg ccgcaggtgc ccgggctcgc gatcacggcg gactattatc
     5401 atatcaagat caccgacgcg atcacgctgc cgacgcccaa cgacgtcttc ggcccctgtt
     5461 tcgaggaggg cgatgcggcg gcctgcgcgc tcatccgccg caatccgctc aacgggtcgc
     5521 tgaacggcgg cggcgatacg ccgggccaga tcgcgctgct caccaatcag gggacgatca
     5581 ccacatcggg ggtcgatttg cggatcaact atgggctacc caccggcttc ggacggctca
     5641 acttcgacct gtcgggcaat tggaccgagc agtcgaagtt caaggcctcg ccgaccagca
     5701 tcaaccgcga atgcgtcggc caatatagct cgaactgcgg cttctttgcc ggcgaaatca
     5761 cgccgaaata cagcttcaac atgcgggcga cggcgcagat cgacgatttc ggcgacatct
     5821 cgctgctgtg gcgctggctc gacggttccg attatgagcg gatattggac gccgacggga
     5881 tcgagcccga ttacctgcat atcccgagct acagctattt cgacctgacc tatcgcgcca
     5941 aggtcgccga catcttcacg ctcacgctgt cggttcagaa cctgctcgac aaggacccgc
     6001 cgctcgtcag caactatgtc ggaacgacgg cgctcaattc gggcaacacc tatccgacca
     6061 cctacgacgt gctcggacgg cgttacagcc tgaccgctag catgcgtttt taagggtcgc
     6121 gcttccggtg cctgggacca gtccccctcg gtgccggccc ggtgtgcgga cgaagggtgg
     6181 tgccttcgtc cgcgcatcac atccgctctc tctctctctc tctctctctc tctctctctc
     6241 tctctctctc tccctcttcc ggcgcgggtg aacgcacgat atctgcggct gaacgtcggt
     6301 cgacagcttc ccgccttcct gcgataaccg cgcgatgaac caggtcgcgg cctttccatc
     6361 aaccggcgtc tacgaagtgg ccacggccgg ccggccgggc agcctcgaac gccagcgacg
     6421 gcaggcgatc ctctggctta ccggcgcctt ctggggcctg cacacgctgg cgctatggat
     6481 cgccgatctg ctcgaccacc acccgcatat cctgcgcgcg accgcggtgc ggttgatgct
     6541 catggcgatc ggcctcgcgc tttgcttcgg cattcacatg atattgcaga agctgagtcg
     6601 gcgggcatgg cgccaaaagg cgccgatagt ctttttcgcc tcgctcgtcg cggccgaact
     6661 cttcgcctgg tgcacctatt tcgccctggt ctatgcgaag ggagagccgg tccagctcgc
     6721 gatcgacggc agcgcggtga tccgcaccct tgttccctgg acgtggtttt ttctggcgtg
     6781 ggtcgggctc tatctggccg ttcaatatgg tttcgaggcg cgcgcggagg aacggcgctc
     6841 ggcgcagctt cgtacgatgg cgcaggcggc caagctgcag gcgctccatt accagatcaa
     6901 cccgcatttc ctgttcaaca gtttcaacgc ggtatcggcg ctgatcctcg acgggcgggc
     6961 cgacgacgcc aatgcgatgg tcgagcggct ggcgggcttt ttccgcgtca accttgccac
     7021 caatcccgac gtcgacattt cgctggcgga ggaggtcgcg ctgcaggcga cctatctgga
     7081 aatcgagcag acgcgttatc ccgatctgga agtccgcatc gacctgccgc cggcgctcgg
     7141 acgcgcggcg gtgccggctt tcctgttgca gccgctggtc gagaacgcgg tcaagcacgg
     7201 cgccgggacg gtcggcgccg gcgacgcgtg gattgcgatc tcggcgcggc aagaacaggg
     7261 ccggctggag gtcgtggtcg aaaacagctg ttcgccatcg cgcgggggcg cggggatttg
     7321 cggcacgcat accggcctcc gcaatgttcg cgaccggctc tccaatcgct ttggcgcgga
     7381 cgcgaaattg tcgatcgagc ggctgcccgc atcccgcttt cgcgcgacgc tcgaactgcc
     7441 gctcggatat gcccgatgac gatgcgggtc ctgaccgtcg acgatgagcc gctcgcgctc
     7501 cgccggctcg aactgatcct cgcgaagcta cccgaaatag agcatgtcgg gtcggcgagc
     7561 ggttgccgcg aagccatcgc gaagatcgcc gagctgaaac ccgatatcgt gctgctcgac
     7621 atcaagatgc gcgacggaac gggtttcgac gtcctcagcc ggctgcccga ggatggggtc
     7681 ccgggggtca ttttcacgac ggcgttcgat catttcgccg ttcgcgcctt cgaagcccac
     7741 gccatcgact atatcctcaa gccgatcgaa atttcgcggc tgcgcgcggc gatcgaccgg
     7801 tcgcgcgacc ggctcgcggc cgccgatgcg cacgaacaaa tcgcccagat gcgcgcggtg
     7861 atcgacgacc tgcgcgcggg ttatcacgag cagggggtaa ggcaacgcga cgacgagctg
     7921 tggctgcgcg ggaccgcggg cgcgctaacc tgtatccagc gcgccgacat cgacatgatc
     7981 accgccgagg atgactatgt ccggctgcac acggcctccg catcctatct gctgcgcggg
     8041 tcgatccggg cggcgcaggc cgacctcgac cttcccgaag aggagttcgt ccgcgtacat
     8101 cgccgcgcgc tgctgcggcg aagcgcgatc gcaagcgtgc ggcgctcggc cgggaagggc
     8161 gtacgcgtga ttctgcgaaa cggcaccgat gtgggcgcgg ggcgggtcta cgccaaaaaa
     8221 ttgctccgtt cgcttcccgc ggtcggctga ccgccggacg cagggggcac gctgcgtttc
     8281 ggtccgatct ggcgtcaggc ctgactggcg gggggctcct cgccgtcgat gccctgatat
     8341 ttgagctgga gatagcgttc gcgcgtcgcg agcttgtcga gttcgcccgc cgcgaggctg
     8401 cgcgcggcgt tgccgatctc gccttcgagc attttgaggc cgccgacgag cgtctcgtcg
     8461 ggggtccggc cggcatgggc ttccttgcgg agacccgcgg ggatgcgctg atagcccgcg
     8521 acgagttcgg gcaaatcctc gccgaccagc ttgcggatat tcgccgccgc ggggctcgat
     8581 gcgtcgagcg tctggagctg cggcgcgagc aggtcgagct ggacgccgat gccgtcgacg
     8641 agctgacgcg cgggagcggg aagcgcgggg cgctgcgctt cgagccagat ttcggtgcgg
     8701 ccggcgagtt gcttcaagtc ggccttgggc aggtcggtct ggcgcggttc ggggaagggc
     8761 ggccatttgc cgaacagcac cgcgacgccg atcaacagcg cgaacagcgc gagcaggccg
     8821 atgaaaccga gcggctggat cagcgcgccg aacaccgcgg cgccgatcag cacgatgccg
     8881 cccgcgatca gcatccggcc gagcttcttg tagagatgct ggcggcgcag cgcgacgctc
     8941 ttggtgccga tcgggcgtcg ccgctcgcgc gaccgttcga tcgccgacgc ggcggtaatc
     9001 cggatcttgt cgacttcgct catgctcatt gcttagcctt cgagcgcggc gagcgggctg
     9061 tcggcgccgc cgacgctggc ctgcgcctgg ctcgcgcctt cggcgcgtgc gatatagccc
     9121 ttcgacttgg cgacctcgcc ctcgagcgtc gt
//
LOCUS       DATMSU010000068         7574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_1513, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000068 DATMSU010000000
VERSION     DATMSU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(736..978)
                     /locus_tag="VN034_01695"
     CDS             complement(736..978)
                     /locus_tag="VN034_01695"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV59337.1"
                     /translation="MPRLGGVDPYLDILGKRIRARRSELGLSQEGLAHEAGLDRSYVG
                     RIERGEHNLTFVALVKLCRAMKCDVAALTGGLPPTR"
     gene            1060..1407
                     /locus_tag="VN034_01700"
     CDS             1060..1407
                     /locus_tag="VN034_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59338.1"
                     /translation="MTKIRFLLTPLAAIAVVTVPVFAAPPKESGPWDLMLVTPKSQAI
                     AVAGITRDKCEEGLRLAVRDGHKAYCARYIDNLKAASPEEQRYSAFLYPVTTPTECLL
                     AKRSLGQVECVYR"
     gene            complement(1450..2394)
                     /locus_tag="VN034_01705"
     CDS             complement(1450..2394)
                     /locus_tag="VN034_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59339.1"
                     /translation="MCADTKLVFSYQLGDRDIPDATAFAKDVASKLKRTKRGEFAVRP
                     LIVTDGLRSYDEAFDLAFGSDADRGVMVKRYDYLVHDGKRSKRKYYVGADREVRVGKP
                     DPADIHTSYIERQNCNLRMGNRRYNRKTNAFSKTLLNHERHLALWIMYHNLCWVPRPS
                     RPPLGEDGKSSGSWIKNLPAGIAAGVTDRLWEIEDLLSLTDEFIAERNRPQQSPENEL
                     AIQPALETVNDAKPTFWVFHHFHQSATRVHRADCPNCRDGHGKKEGTSKYGEWTPFHS
                     QESAVEAARQVQPDRNSICKICLGSYYTLGYRHPKMPS"
     gene            complement(2883..3071)
                     /locus_tag="VN034_01710"
     CDS             complement(2883..3071)
                     /locus_tag="VN034_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59340.1"
                     /translation="MKKRKKANSAALVRAARHLADEANHDAAPLTDEERAIMDEVRGE
                     HSRPSKEEIRKEAKRLLK"
     gene            complement(3068..3256)
                     /locus_tag="VN034_01715"
     CDS             complement(3068..3256)
                     /locus_tag="VN034_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59341.1"
                     /translation="MHTRNPSIHLTRGRQTLTITKLSGRYIGLINGNPYIEALCPDEV
                     VRHLVQAPSNDNGIGDKQ"
     gene            3441..3785
                     /gene="rpoZ"
                     /locus_tag="VN034_01720"
     CDS             3441..3785
                     /gene="rpoZ"
                     /locus_tag="VN034_01720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWV59342.1"
                     /translation="MARVTVEDCVDKIPNRFDLVLLSAHRAREISGGSELTVDRDRDK
                     NPVVALREIAEQTVRPKDLQETLVGSMQKVVVDDDDTPDEISSISRSAEALRLTAAAP
                     PRSPAGGGSDFE"
     gene            complement(3838..4443)
                     /locus_tag="VN034_01725"
     CDS             complement(3838..4443)
                     /locus_tag="VN034_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HWV59343.1"
                     /translation="MTIYCDESGGLNAGAMTFSAVMLTPQTATEIHDRFRSVTGLRGE
                     LKGSRISIVERAYLLELFDRAGGRAWVAVAKRDQLAHNPGGTLPSDLALYAGLLNLAI
                     GRWLPETGGVCTDVVIDDGRYDPKILAHVREEIQAGLGQWGRASLADSRRSDGVQIAD
                     VIANSLFNIAVGSQRAHRIQKILDPMLASKAIRIAELSELS"
     gene            complement(4453..4938)
                     /locus_tag="VN034_01730"
     CDS             complement(4453..4938)
                     /locus_tag="VN034_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59344.1"
                     /translation="MIGKVGNILAAAALLACSAGPAAAQDTGALTSKIELEKSTPAAD
                     GQPANKTYVAPDVVVPGDRVRVTLTFTNKGAAAASGVNLVNPIPEGLAFDDTDDSAGF
                     GVSIDGGKNFGPLAALTVPVAAGAPRPAAATDVTHVRWLWPDAIAPGASRSVAFFGRV
                     R"
     gene            complement(4974..5384)
                     /locus_tag="VN034_01735"
     CDS             complement(4974..5384)
                     /locus_tag="VN034_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59345.1"
                     /translation="MAKGSLRRMINANHARILPLLLAAPLLCAMAPGDMSVAAFLARA
                     ASIERLGPLALATPQGHQLKSEVVAAGKRYKARIDAERRAGRKTTSCPPESGSLTPEQ
                     WLSHLRSYPAAVRRKVSIYSAFDALMKKRYPCPA"
     gene            complement(5421..7364)
                     /gene="ftsH"
                     /locus_tag="VN034_01740"
     CDS             complement(5421..7364)
                     /gene="ftsH"
                     /locus_tag="VN034_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWV59346.1"
                     /translation="MQDDKEPQGNPWMKSVMIWSGILLAMLLVASMFGGAAQPAGSPL
                     AYSEFRQKVEEGSVKDVVLSEDKVTGTLSNGDRFTANVVRDPELLKMLNDNGVKYDGK
                     AAEVPNFWMYMLVQSLPFLLILGIAFFVFRQVQKNNGSGAMGFGKSRAKMLTEKQGRV
                     TFDDVAGIDEAREELEEIVEFLKDPTKFSKLGGQIPKGALLVGSPGTGKTLLARAIAG
                     EAGVPFFTISGSDFVEMFVGVGASRVRDMFEQAKRNAPCIVFIDEIDAVGRHRGAGLG
                     NGNDEREQTLNQLLVEMDGFEANEGIIIVAATNRPDVLDPALLRPGRFDRQVVVPRPD
                     IEGRQKILEVHTRKKPLAPDVDLRRVARGTPGFSGADLANLCNEAALLAARKGKRLIA
                     SNEFEEAKDKVMMGAERRSMVMTEDEKKATAYHEAGHALVSLHVEGNDPLHKVTIIPR
                     GRALGVTWNLPERDRYSTNMKQMKARLALCFGGRIAEQLIYGKDELNTGASNDIQQAT
                     DMARSMVMEYGMSEKLGWLRYRDNQDEVFLGHSVSRAQNMSEETAKLIDAEVRRLVEE
                     GENVARKVLTDNIDELHLLAAALLEYETLSGEESKRAIKGEDIGREDDADKRGTPVSG
                     HAGGLPQIKRKPRPFGDANPQGA"
BASE COUNT         1413 a         2247 c         2347 g         1567 t
ORIGIN      
        1 cgatcccacc gatatcggcg ccgacgagat caacgcacgc atggcgaagc tcgccaagcg
       61 acgagcacag ggaagagccg cctacgccga cgctaaatcc cgcgacagct aatcgatggc
      121 ggcttcctga ccacgcttac gacctcagat cgatttccaa acttgatctt tccgaagcca
      181 atctagaatg ctctatcgga gaggggtttc caatttggca tatcgaatgc aataagctca
      241 cgacgcaatt gggaagggaa gggctgaaat gcagtcgagc gattcagagc aggcactggt
      301 aaaggtgctg gaacggctca atgataccat ttcggaaaac aacaaaatct tattgggact
      361 cgaagacgtc ctgcaaacca tttcggcaaa gctatctgat gtcgcaggaa attcgagcta
      421 cgggtcctag tatatattcc gcgtaaattt gggcgcgggc taggatggct tccgtactct
      481 ctccgatttt tgcagtttgc atcgcccagc gcatagcgtt ctctcgggcc acgccttcag
      541 ctcgcataat attaaatcgc gtatcagtca tcgtaaggca attccttgca aggaaggcaa
      601 gcgtaaaagg gctgcaacgg ctatattccg ttaagtcagt ggcttgcaat ttagtggggt
      661 ggcgcagctt aagcacgtga cagaaattat actataaaac gccagccttg tcaagaaaat
      721 tggctgactt tggagctatc gcgtgggagg tagtccgccc gtcagcgccg ccacatcaca
      781 cttcatcgcc cgacatagtt tcaccaacgc gacgaacgtc agattatgtt cgccgcgctc
      841 gatccggccg acatagctgc ggtccagccc tgcctcgtgg gcaagccctt cctgtgacaa
      901 gccaagctcg cttctgcggg ctcgaatgcg tttgccgagg atgtctagat acgggtccac
      961 accgccaagt cgcggcacag gggagtatca gtccacggag taagaagccc gctgttaacc
     1021 tttgagcact atttgcgact ctgcaaagga gatacagccg tgaccaaaat tcgtttcctg
     1081 cttaccccac tcgccgccat tgctgtcgtg acagtccccg tcttcgctgc cccgccgaag
     1141 gaaagcggac cgtgggattt gatgcttgtc acgccaaagt cgcaggcgat cgcggtagcc
     1201 ggcataacgc gggataagtg tgaggaaggg ttgcgccttg ccgtccgcga tggtcacaag
     1261 gcatattgcg ctcgatacat cgacaacttg aaagcggcgt ctccggaaga gcagcgctac
     1321 agcgctttcc tctatccagt gacgacgcca accgaatgcc ttctcgccaa acgatcgctc
     1381 gggcaggtcg aatgcgtata tcggtaaccg attgagtagg tgaccgctct gatcgtttac
     1441 gcagagcgcc tatgacggca ttttggggtg ccggtagccg agcgtgtaat acgagcccaa
     1501 gcagattttg cagatcgagt ttcggtcggg ctgcacttgc cgggcggctt cgactgcgct
     1561 ctcttgcgaa tggaatggcg tccactcgcc atatttcgac gtgccctcct tcttgccgtg
     1621 cccgtcgcgg caattggggc aatccgcgcg atgaactctc gtcgcgctct gatgaaagtg
     1681 atgaaacacc cagaaggtgg gcttcgcgtc attgaccgtt tccagcgccg gttggatcgc
     1741 caactcgttc tcgggcgact gctgcggccg atttcgctcg gcaatgaact catcggtcag
     1801 cgagagcagg tcttcaatct cccacaaacg atcggtcacg ccggccgcaa taccggcagg
     1861 aaggttcttg atccacgatc cggaggactt tccgtcctct ccaagaggcg ggcggcttgg
     1921 gcgcggaacc cagcagagat tgtgatacat gatccacagc gcgaggtggc gttcgtggtt
     1981 caacagcgtc ttggaaaatg cgttggtttt gcggttatag cgtcgattgc ccatccgcag
     2041 attgcaattc tggcgctcga tatagctggt gtgaatatcg gccggatcgg gcttgcctac
     2101 tcggacttcg cggtctgctc caacatagta tttccttttg ctacgcttcc cgtcgtggac
     2161 cagatagtcg tatcgcttca ccatgacgcc gcgatcggca tcgctgccga atgccagatc
     2221 gaacgcttca tcgtaggacc gcaagccgtc agtcacgatc agaggtcgaa cggcaaattc
     2281 accccgctta gttcgcttga gcttggacgc gacatccttc gcaaacgcag tcgcgtcggg
     2341 aatgtcgcgg tctccgagtt ggtatgagaa gacgagtttc gtatcggcgc acatcgccag
     2401 ataagcccat tgctcccagc atcgtcggat ggggacacca tacgcggaac attccgcttc
     2461 ttggctgcaa cgaatgcgtg gagttcgtcg gcctgaatcc gcttcggcgt caggtcgcgt
     2521 agcgacgcga catgagcgat ggccatgtcg ccggcttcct cgaacagttt ggaaacggtc
     2581 ttgttgctga cccggaagat acgctcgcac gagcgctggg acatgccttc gcaaagactt
     2641 tggagaatat aggcgcgggt tcgtgcatct agtctattca tccggtgccg atgctgaccg
     2701 gtcccggaca gtcccaatct tgatgatagt catattcgat ctccttgcag tggcgtggct
     2761 attccgcgcg cggggcttgg agatgagaac aggacggcaa tcaagaaagg aattgtgctg
     2821 aatgaaaaat ctgtattaga gttggatgta aggctctgaa aagattaacc ttaacgctct
     2881 tcctatttga gaaggcgctt cgcctctttg cgaatttcct ctttgctagg gcgcgagtgt
     2941 tcgccacgaa cctcgtccat aatcgcccgc tcttcgtcgg tcagaggggc tgcatcgtga
     3001 ttggcttcat cagcaagatg ccgggcagca cggacgaggg ctgcggaatt ggccttcttc
     3061 cttttcttca ctgcttatct ccgatgccat tgtcattcga cggtgcctgc acgagatgac
     3121 gcactacctc gtcggggcac agagcctcaa tatagggatt gccgttgatg aggccaatgt
     3181 agcgtccgga taatttcgta atggtgagcg tctggcggcc acgagtgaga tggatgctgg
     3241 gattacgtgt gtgcatatga tcgactcctt ctggatcgtc acatgcctcg gcctgccgag
     3301 ccttgttttg caaccagcta aatcaaacta cgtcaccacc agagcgccgc tttggttgac
     3361 atcggcgggc ctccctgcta gcgcgcagcg cttgccgcca tgccccaaag gctggtctct
     3421 ccgatattta aggaaacaat atggcccgcg ttaccgtcga ggattgcgtc gacaagattc
     3481 ccaaccgctt cgacctggtt ctgctttccg cgcatcgcgc gcgtgaaatc tcgggcggtt
     3541 cggagctgac cgtcgatcgt gaccgcgaca agaaccccgt cgtcgcgctg cgcgagatcg
     3601 ccgaacagac cgttcgcccc aaggatctgc aggaaacgct cgtcggttcg atgcagaagg
     3661 ttgtcgtcga cgatgacgat acgcccgatg aaatcagctc gatcagccgt tcggccgagg
     3721 cacttcgctt gaccgccgcg gctccgccgc gcagcccggc aggcggcggc agcgacttcg
     3781 aataagcacg gcttgcacga atcaccagga gggccgcggg aaaccgcggc ccttttgtta
     3841 tgaaagctcg ctcaactcgg cgatgcggat cgccttcgac gcaagcatcg gatcgaggat
     3901 tttctggata cgatgcgcgc gctgcgagcc gaccgcgatg ttgaacaggc tgttcgcgat
     3961 cacgtcggcg atctggacgc cgtcgctgcg gcggctgtcg gcgagcgaag cgcggcccca
     4021 ttggccgagc cctgcctgaa tctcttcgcg gacgtgcgcg agtatcttgg ggtcgtaacg
     4081 cccatcgtcg atcacgacgt cggtgcacac gccgcctgtt tcaggcagcc agcgcccgat
     4141 cgcaagattg agcagtccgg catagagcgc gagatcgctc ggtagcgtac cgccgggatt
     4201 gtgggcgagc tggtcgcgct tggcaaccgc gacccatgcc cggccgccgg cgcggtcgaa
     4261 gagctcgagg agataggcgc gctcgacgat agagatgcgg ctgcccttga gctcgccgcg
     4321 caatccggtg acgctgcgga accggtcgtg gatttcggtc gcggtttggg gcgtcaacat
     4381 caccgccgag aaggtcatcg cgcccgcgtt gagcccgccc gattcgtcgc aatagatcgt
     4441 cattccgagg ggttaccgca cgcgtccgaa aaaggcgacc gagcggctcg cgccgggcgc
     4501 gatcgcgtcg ggccagagcc agcggacatg cgtgacatcg gtcgccgcgg cgggacgggg
     4561 cgcgccagct gcgaccggca cggtgagggc ggcgagcggg ccgaaattct tgccaccgtc
     4621 gatcgagacg ccgaatcccg cgctatcatc ggtgtcgtcg aaggccaggc cttcgggaat
     4681 gggattgacc agattgacgc ccgacgcggc ggcggcaccc ttattggtaa aggtgagcgt
     4741 gacgcgaacg cggtcgccgg gcacaacgac atcgggcgcg acataggtct tgttcgcggg
     4801 ctggccgtcc gcggcgggcg tcgatttctc cagttcgatc ttgctcgtca gcgcgccggt
     4861 gtcctgcgcc gcggccggtc cggcgctgca ggccagaagc gccgccgcgg ccaatatatt
     4921 ccccaccttg ccgatcatgg gcatctcctt cggtccgctc gcaccttggc gtatcaggcg
     4981 gggcagggat agcgtttctt catcagggca tcgaaggctg aatagatgct caccttcctg
     5041 cgcacggcag ccggatagct gcgcaggtgc gagagccatt gctcgggggt gaggctgccc
     5101 gattcgggcg gacaactcgt ggtcttgcgg ccggcacgcc gctcggcgtc gattcgcgcc
     5161 ttgtagcgtt tgcccgccgc gacgacttcg cttttgagtt gatggccttg cggcgtcgcg
     5221 agcgcgagcg gaccgagccg ttcgatcgat gccgcgcgcg ccagaaacgc cgctacgctc
     5281 atgtcgcccg gcgccatggc gcagaggagc ggagcggcga gcaggagagg caggatgcgc
     5341 gcatggttcg cgttgatcat gcgccgcaag ctgcccttcg ccaatgaatg atcgtctaaa
     5401 tcgacgggac tcgtcgaggc ctaagctccc tgcggattgg cgtcgccgaa cgggcgcggt
     5461 ttgcgcttga tctggggtag gccgccggcg tggcctgaaa ccggggtgcc gcgcttgtcg
     5521 gcatcgtcct cgcggccgat gtcctcgccc ttgatcgcgc gcttcgattc ctcgcccgac
     5581 agcgtctcat attcgagcag cgcggcggcg agcagatgca gctcgtcgat attgtcggtg
     5641 agcaccttgc gcgcgacatt ctcgccttcc tcgacgaggc ggcggacttc ggcgtcgatc
     5701 agcttggcgg tttcttcgga catgttctgc gcgcgcgaga cgctgtggcc gaggaaaacc
     5761 tcgtcctgat tgtcgcgata gcgcagccag ccgagtttct cggacatgcc atattccatg
     5821 accatcgagc gtgccatgtc ggtcgcctgc tggatgtcgt tcgacgcgcc ggtgttgagc
     5881 tcgtccttgc cgtagatcag ctgctcggcg atgcggccgc cgaagcagag tgcgagacgc
     5941 gccttcatct gcttcatatt ggtcgaatag cggtcgcgtt cgggcaggtt ccacgtcacg
     6001 cccagcgcac ggccgcgcgg gatgatcgtg accttgtgga gcgggtcgtt gccctcgacg
     6061 tggagcgaga cgagcgcgtg gccggcttcg tgataggcgg tcgccttctt ctcgtcctcg
     6121 gtcatcacca tcgagcggcg ttcggcgccc atcatgacct tgtccttggc ttcctcgaac
     6181 tcgttcgatg cgatcaggcg cttccccttg cgcgcggcga gcagcgccgc ttcgttgcaa
     6241 agattagcca gatcggcgcc cgagaagccc ggggtcccgc gcgcgacacg gcggaggtcg
     6301 acgtcgggcg cgagcggctt cttgcgcgtg tggacttcga ggatcttctg gcggccctcg
     6361 atgtcggggc gcggtacgac aacctggcgg tcgaagcggc ccggacggag gagggcgggg
     6421 tcgagcacat cggggcggtt ggtcgccgcg acgatgatga tgccctcgtt cgcctcgaaa
     6481 ccgtccattt cgacgaggag ctggttcagc gtctgctcgc gctcgtcgtt gccgttgccg
     6541 aggccggcac cgcggtggcg gccgaccgcg tcgatttcgt cgatgaagac gatgcacggc
     6601 gcgttgcgct tcgcctgttc gaacatgtcg cggacgcggc tggcgccgac gccgacgaac
     6661 atttcgacga aatccgaacc cgaaatggtg aagaagggga cgcccgcttc acccgcgatc
     6721 gcgcgggcga gcagggtctt gccggtgccg ggcgaaccga cgagaagtgc gcccttggga
     6781 atctggccgc cgagtttcga aaatttggtc gggtctttca agaattcgac gatttcttcg
     6841 agttcctcgc gtgcttcgtc gatgcccgcg acgtcgtcga acgtcacgcg gccctgcttt
     6901 tcggtgagca tcttggcgcg cgacttgccg aaacccatcg cgcccgagcc attgttcttc
     6961 tgcacctgac ggaagacaaa gaaggcgatg ccgaggatca ggaggaaggg cagcgactgg
     7021 acgagcatat acatccagaa attcggcact tcggccgctt tcccgtcata tttgacgcca
     7081 ttgtcgttga gcatcttcaa aagttcgggg tcgcgcacga cgttggcggt aaagcggtcg
     7141 ccgttcgaca gcgtgccggt caccttgtct tcggagagga cgacgtcctt gacgctgcct
     7201 tcctcgacct tctggcggaa ttccgaatag gcgagggggc tgcccgcggg ctgcgcggcg
     7261 ccgccgaaca tcgaggcgac gaggagcatg gcgagcagga tgccgctcca gatcatcacg
     7321 ctcttcatcc aggggttgcc ctgcggttcc ttgtcgtcct gcattcggat tcgtcctcaa
     7381 cggcgcggtg ctgcgctcct atctgcacaa gataggataa tcgggttaaa tgacaatgag
     7441 gcaatcaggg ctttttaggg attttccgcc gcggcgccgc ggaaatgcgc cagatcgtcc
     7501 cttcgagacc gcgtacggcg tcgatcagca gcgcgccgac catcgcgcgg cggccctggt
     7561 gcatcgcggc gatc
//
LOCUS       DATMSU010000069        10477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181578,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000069 DATMSU010000000
VERSION     DATMSU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181578"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /gene="infB"
                     /locus_tag="VN034_01745"
     CDS             <1..1941
                     /gene="infB"
                     /locus_tag="VN034_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWV59347.1"
                     /translation="PKRPEPKANRGADNRRQSGKLTVTRALNEDEGARARSLAALKRA
                     REKEKRSHTASSGPREKQVREVVVPDSITVQELANRMAEKGADLVKALFKMGMPVTVN
                     QTIDQDTAELLVTEFGHEIKRVSDADIDIRHDEDVDDTAALKPRAPVVTIMGHVDHGK
                     TSLLDALRGANVVSGEAGGITQHIGAYQVKTPDGSEVTFLDTPGHEAFTEMRQRGANV
                     TDIVILVVAADDGLKPQSIEAIQHAKAAGVPIIVAINKVDKEGANPQRVRERLLEHEL
                     VVEEMGGDVQNVEVSALKKTGLDKLLDAIAVQAEIMELKANPDRAAEGTVIEAKLDKG
                     RGPVATILVRRGTLKVGDIFVCGAESGRVRALVDDHGKQIKTAGPSMPVEVLGLGGVP
                     MAGDTLTVVENEARAREVAAYRQEQALRKRTAQAPVSLEGMFEALADKANVIQFPVVI
                     KGDVQGSVEAIVNALNKISTDEIKVRVLQSGAGAITESDVTLAAASNAPIIGFNVRPN
                     AKAREIAKREKVRFMYHDVIYHLTDEVRKEMAGELGPERIENVVGRAEVKDVFPAGKR
                     DKAAGLLVLEGSIRKGLHARLTRDDVIVSATTIASLRRFKDDVAEVRAGLECGVVLAD
                     TNDIKAGDHLEVFEVELRERTL"
     gene            1978..2535
                     /locus_tag="VN034_01750"
     CDS             1978..2535
                     /locus_tag="VN034_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWV59348.1"
                     /translation="MARYVALFGSINVGGNRLKMVDLRSAFEAEGFSDVETVVASGNV
                     IFSHPARPTRGLEEKLTLMVDDRFDMRCAALVRSRDELAAAIADNPFAGTNEDKFVHT
                     MFLDGQPTAAQFDALAGDKWIKPNERLALGDRALYIDYGDGVADSKLTGRLIERRLKH
                     KGTARNMRSIARIVDKLDQLEKARP"
     gene            2532..2942
                     /gene="rbfA"
                     /locus_tag="VN034_01755"
     CDS             2532..2942
                     /gene="rbfA"
                     /locus_tag="VN034_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWV59349.1"
                     /translation="MKPDKDRKRDSGPSVRVLRVGEQVRHLLSEILARGDVHDDVLAT
                     HPVSITEVRMSPDLRHATVFVKSLLGKDEEAVLKALRTNTAWLQKSVAEKMSMKYAAK
                     LKFLSDESFDEASHIDALLRDPKVARDLESGEGE"
     gene            2939..3646
                     /locus_tag="VN034_01760"
     CDS             2939..3646
                     /locus_tag="VN034_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59350.1"
                     /translation="MKQVAPLVIATLVAIGVALSPAFLSPQFVRPSRGDALDIIEAAS
                     QYSLSVQNAEAGGQAAPPMPALAKPPHIRFIETKPCGATSASEDCVVIDVHLGEETGR
                     LLETSWHDAAVNLERYADITSENPFPKPIDTRRLQSPVAARLARLQIAASRQELDFIA
                     PSGLATADVLEGISLRDDMPADHIRIYSAPWAAGDIAFIEVGLICGDLCGRGENYALR
                     KIDGTWRVIAVQPSWVS"
     gene            complement(3658..5076)
                     /locus_tag="VN034_01765"
     CDS             complement(3658..5076)
                     /locus_tag="VN034_01765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWV59351.1"
                     /translation="MRLMPVTDAMFLWGESREMPMHIGGINLYTLPDGVDETEWLNEQ
                     LALLRQSEGLRRPFSEVLKLTPFGQYGPIRLTPDRDIDMHYHVRAAALPKPGRYREMF
                     ELASRLHSGLLDRTRPLWEITLISGLPNRQIATFKKVHHALMDGAASIHFYNSMLSAD
                     PEERRAASPLSVEAYEAYKRQFPPPKKPARPNLTDIKAIGDFLKEQWGNSVGVTKAMN
                     QYLAAMFGIGGDGLATPFAGVPRTSFNRNITGARRFVAQSWSLDRVRAMGKAYDGTIN
                     DAVLGMCAGAMRKYLQSLNELPDKPLKAMAPVSIRPKDDVDSGNSVASVTANLATHID
                     DPATRMAMIQESMNSAKAQLRAMTAAQIQLYTAITSFPMMLTALTRTADKFPAYSVTI
                     SNVPGPREQMYWNGARLDGMYPASIPVDGMAMNITQVSNFKNIDFGITACRRSVPHAQ
                     RMIDYLEEALVELEAAAGIKKG"
     gene            5202..5804
                     /locus_tag="VN034_01770"
     CDS             5202..5804
                     /locus_tag="VN034_01770"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HWV59352.1"
                     /translation="MAKLYFYYASMNAGKSTTLLQADFNYRERGMETMLWTAALDDRY
                     GAGQITSRIGLLAEAHKFDPDSDMFAAVTGENRQRPLACVLVDEAQFLSREQVLQLAR
                     LADEADIPVLCYGLRTDFAANLFPGSAALLGLADALVELKAVCECGRKATMNLRVDEK
                     GRAVVEGAQTEIGGNDRYVAMCRRHFMAKRREAAEELADA"
     gene            5797..6333
                     /locus_tag="VN034_01775"
     CDS             5797..6333
                     /locus_tag="VN034_01775"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWV59353.1"
                     /translation="MLDRLYTELADGDLRLVKLAEPHREALRAACAADADVWPIYSSS
                     FDPEHFDQSFDTLVGGKFRMPYAIFDGDTLVGMTAWLRPDMSAQTVEIGNSYIHPAAR
                     GTGLNGRLKKLMLDHAFAVGIRRIEFRIDERNKRSQAAVAKLGCTKEGVLRSERITWT
                     GYLRDTGLWSLLADEWRR"
     gene            6390..6797
                     /locus_tag="VN034_01780"
     CDS             6390..6797
                     /locus_tag="VN034_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HWV59354.1"
                     /translation="MKPKLALSLAVLLLPLSAAAAIEPAEEPRALGVESSIVFPSDSS
                     IRNWKADRGRGIWIEGRRGEWYYGTFAGICRDLDFAQAIGVETRGAGRLDKFASIIVR
                     GERCMLSSFVTSAPPPSKRDKAKDIMGEKPAAN"
     gene            6831..7823
                     /gene="truB"
                     /locus_tag="VN034_01785"
     CDS             6831..7823
                     /gene="truB"
                     /locus_tag="VN034_01785"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWV59355.1"
                     /translation="MNGWIILDKPVGLGSTQAVGAVKRVCREAGLGKVKVGHGGTLDP
                     LASGVLPIALGEATKLCGRMLDASKIYDFTVQFGTETAGLDAEGEVVATSDVRPTLAE
                     IEAVLPRFTGPIEQVPPAYSAIKVGGQRAYDLARKGEVVEMKARAVTVYSLKPLPFRG
                     GVGVGAAGVRGADAHPAATSEQARKSRCPSPEGEGLNEITITAHVSKGTYIRSLARDI
                     ARAVGSVGHVTMLRRTRAGPFTLEQAISLDKLNAFGQGAAQSEIILPLEAGLVDIPAL
                     NLSPEAAGAIRQGRVWTGGSTDDGLYWGRDSDMRPVALIEALAGTLKVVRGFNL"
     gene            7850..8119
                     /gene="rpsO"
                     /locus_tag="VN034_01790"
     CDS             7850..8119
                     /gene="rpsO"
                     /locus_tag="VN034_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWV59356.1"
                     /translation="MSITAERKAEVIKDNAREKGDTGSPEVQVAILTDRINTLTDHFK
                     AHHKDNHSRRGLLMMVNKRRSLLDYLKKKDEARYSALIAKLGLRK"
     gene            8312..>10477
                     /gene="pnp"
                     /locus_tag="VN034_01795"
     CDS             8312..>10477
                     /gene="pnp"
                     /locus_tag="VN034_01795"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWV59357.1"
                     /translation="MFDVKKVSIEWGGETLTLETGKVARQADGAVMATLGETVVLCAV
                     TAAKSVKDGQDFFPLTVHYQEKYSAAGRIPGGFFKRERGATEKETLVSRLIDRPIRPL
                     FPEGFYNEINAIAQVLSYDGHNEPDILAMVAASAALTISGVPFMGPIGAARVGYIDGE
                     YILNPTDAQVAEGDLDLVVAATYDAVMMVESEANELSEEVMLGAVLFAHDACKEVVKA
                     IVKLAEQAAKDPWDMAASDDNAGIKDKLKKLVGKDITAAYKLTDKSARSNALNEARAK
                     AKESFAADGLSPQEVMAGIKLVKKLEAEIVRGAILKDGKRIDGRTTTQIRPIVAETHF
                     LPRAHGSALFTRGETQTIATCTLGTKDAEQMIDGLNGLSYQNFMLHYNFPPYSVGEVG
                     RFGAPGRREVGHGKLAWRALHPVLPTKDEFPYTIRLTSDITESNGSSSMASVCGGSLA
                     MMDAGVPIKRPVSGIAMGLILEGKDYAILSDILGDEDHLGDMDFKVAGTADGITTMQM
                     DIKIAGITREIFEAALNQAKEGRAHILGEMAKALGEARTELSAHAPRIETMQIDKSKI
                     RDVIGTGGKVIREIVATTGAKVDIDDEGLIKISSSDLDQIEAARKWISGIVEEAEVGK
                     IYDGKVVNLVDFGAFVNFMGGKDGLVHVSEIANERVEKVADVLSEGQEVKVKVLEIDP
                     RGKVRLSMRVVNQETGEELEDTRPPREPRGDRGPRRDGGD"
BASE COUNT         2022 a         3303 c         3429 g         1723 t
ORIGIN      
        1 cccaagcgcc ccgagccgaa ggcgaaccgc ggtgccgaca atcgccgcca gtcgggcaag
       61 ctgaccgtca cgcgcgcgct caacgaggat gagggcgccc gcgcgcgcag cctcgcggcg
      121 ctgaagcgcg cccgcgaaaa ggaaaagcgt tcgcacaccg cctcgtcggg gccgcgcgaa
      181 aagcaggtcc gcgaagtcgt cgttcccgac agcatcacgg tgcaggaact ggcgaaccgc
      241 atggccgaaa agggcgccga tctggtcaag gcgctgttca agatgggcat gccggtcacc
      301 gtcaaccaga cgatcgatca ggacaccgcc gagctgctcg tcaccgaatt cgggcacgag
      361 atcaagcgcg tgagcgacgc cgatatcgac atccgtcacg acgaggatgt cgatgacacc
      421 gcggcactga agccgcgcgc gccggtcgtg acgatcatgg gccatgtcga ccacggcaag
      481 accagcctac tcgacgcgct gcgcggcgcc aatgtggtgt cgggcgaagc gggcggcatc
      541 actcagcata tcggcgccta tcaggtgaaa acccccgacg ggagcgaagt caccttcctc
      601 gatacgccgg gtcacgaagc ctttaccgag atgcgccagc gcggggcgaa cgtcaccgac
      661 atcgtcatcc tggtggtggc ggccgatgat ggtctcaagc cgcagtcgat cgaggcgatc
      721 cagcatgcaa aggccgcggg cgtgccgatc atcgtcgcga tcaacaaggt cgacaaggaa
      781 ggcgccaatc cgcagcgcgt ccgcgaacgc ctgctcgaac atgagctggt ggtcgaggaa
      841 atgggcggcg acgtccagaa tgtcgaagtg tcggcgctca agaaaacggg gctcgacaag
      901 ctgctcgacg cgatcgcggt gcaggccgag atcatggagc tcaaggccaa tcccgaccgc
      961 gccgccgaag gcacggtgat cgaggcgaag ctcgacaagg gtcgcggccc cgtcgcgacg
     1021 atcctcgtgc gccgcggcac gctgaaggtc ggcgacatct tcgtctgcgg cgccgaaagc
     1081 ggccgtgtcc gcgcgctcgt cgacgaccat ggcaagcaga tcaagaccgc cggaccgtcg
     1141 atgccggtcg aagtgctcgg cctcggcggg gtgccgatgg cgggcgacac gctgacggtg
     1201 gtcgagaatg aggcacgcgc ccgcgaggtc gctgcctatc gtcaggaaca ggcgcttcgc
     1261 aagcggacgg cgcaggcgcc cgtcagcctc gaaggcatgt tcgaggcact cgccgacaag
     1321 gccaatgtca ttcagttccc cgtggtcatc aagggcgatg ttcagggcag cgtcgaggcg
     1381 atcgtcaacg cgctcaacaa gatatcgacc gacgagatca aggtccgcgt gctccagtcg
     1441 ggcgccgggg caatcaccga aagcgacgtg acgctggcgg ccgcgagcaa tgcgccgatc
     1501 atcggcttca acgtccgtcc gaatgccaag gcgcgagaga tcgcgaagcg cgaaaaagtg
     1561 cgcttcatgt atcatgacgt catctatcac ctgaccgacg aggtgaggaa ggagatggcg
     1621 ggcgagctgg gtccggaacg catcgaaaat gtcgtcggcc gcgccgaggt caaggacgtc
     1681 ttcccggccg gcaagcgcga caaggccgcg ggtctgctcg tgctcgaagg ctcgatccgc
     1741 aaggggcttc acgcgcgtct cacgcgcgac gacgtcatcg tttcggcgac gacgatcgcc
     1801 tcgctccgcc gcttcaagga cgacgtcgcc gaagtgcgcg ccggcctcga atgcggggtc
     1861 gtgctggcgg atacgaacga catcaaggcg ggcgaccacc tcgaagtctt cgaggtcgaa
     1921 ctgcgcgaac ggacgctgta acaggcgtcc gctccgcgcg gatcaggagg tgccggcatg
     1981 gctcgctatg tggccctatt cggcagcatc aacgtcggtg gcaaccggct gaagatggtc
     2041 gatctgcgtt cggcgttcga agcggagggc ttcagcgacg tcgaaaccgt cgtcgccagc
     2101 ggcaatgtca tcttctcgca ccccgcgcgg ccgacgcgcg ggctcgagga gaaattgacg
     2161 ctgatggtcg acgaccgctt cgatatgagg tgcgccgcgc tcgtgcggag ccgcgacgag
     2221 cttgctgcgg cgattgccga caatccgttc gcgggcacga acgaggacaa gttcgtgcac
     2281 accatgttcc tggatgggca gccgaccgcc gcgcagttcg acgcgttggc gggcgacaaa
     2341 tggatcaagc cgaacgagcg tctcgcgctc ggcgaccgcg cgctctatat cgattatggc
     2401 gacggcgtgg ccgacagcaa attgaccggg cgtctgatcg agcgccggtt gaaacataag
     2461 ggcacggcgc gcaacatgcg ctcgatcgcc cggatcgtcg acaagctcga ccagttggag
     2521 aaagcacgcc catgaaaccc gacaaggacc ggaaaaggga tagtgggccc tccgtccgcg
     2581 tgctgcgcgt cggcgaacag gtgcgccacc tcctctccga aatcctcgca cgcggcgacg
     2641 tccatgacga tgtgctcgcg acgcacccgg tgagcatcac cgaagtccgc atgtcgcccg
     2701 acctccgcca cgcgaccgtc ttcgtcaaat ccctgctcgg caaggacgaa gaagcggttc
     2761 tgaaggcgct ccgcaccaac accgcctggc tgcaaaagag cgtcgccgag aaaatgagca
     2821 tgaaatatgc cgcgaagctc aaattcctgt cggacgaaag cttcgacgag gcgagccata
     2881 tcgatgcgtt gctgcgcgac ccgaaagtgg cgcgggattt ggagagcggc gagggcgaat
     2941 gaaacaggtt gcgccgctcg taatcgctac gctggtggcg atcggagtgg cattatcgcc
     3001 tgcctttctg tcaccgcagt tcgtgcggcc gtcgcgcggc gatgcacttg atatcatcga
     3061 agccgcgtcc caatacagcc tttcggtcca gaatgccgag gcgggcggtc aagccgcgcc
     3121 gcccatgcct gccttggcga aaccgccgca tatcaggttc atcgagacga agccgtgcgg
     3181 cgcgacctcg gcatcagagg actgcgtcgt cattgatgtg catctcggcg aggaaacggg
     3241 ccggttgctg gaaacatcgt ggcacgacgc cgcggtaaac ttggagagat atgcggacat
     3301 cacgtccgaa aatcctttcc ccaaaccaat cgatactcgt cgcctgcagt cacctgttgc
     3361 ggccaggctc gcaagattgc agatcgctgc atcgcggcag gagcttgatt tcatcgcgcc
     3421 gtccggactg gccacggcgg atgttctgga aggcatatcc ctgcgcgacg atatgcccgc
     3481 ggatcacatt cggatatatt cagctccgtg ggcggccggc gatatcgcgt ttatcgaggt
     3541 cggtttgatc tgcggcgatc tttgcgggcg cggggaaaat tacgcactcc gcaagattga
     3601 cggcacgtgg cgcgtcatag cagtccagcc cagctgggtt tcgtaagcgt agggctacta
     3661 ccccttcttg atccccgccg ccgcttccag ctcgaccagc gcttcctcca gatagtcgat
     3721 catccgctgc gcgtgcggca cgctgcggcg gcacgcggtg atgccgaaat cgatattttt
     3781 gaagttcgac acctgggtga tgttcatcgc catgccgtcg acggggatgc tcgcgggata
     3841 catgccgtcg aggcgcgcgc cgttccagta catctgttcg cgcggcccgg ggacgttcga
     3901 aatcgtcacc gaataggcgg ggaatttgtc ggccgtgcgc gtcagcgcgg tcagcatcat
     3961 cgggaagctg gtgatcgcgg tatagagctg gatctgcgcc gcggtcatcg cgcgcagctg
     4021 cgccttcgcc gaattcatcg attcctggat catcgccata cgcgtcgcgg gatcgtcgat
     4081 atgcgtcgcg agattggcgg tcaccgaagc gaccgaattg cccgaatcga catcgtcctt
     4141 gggccggatc gataccggcg ccatggcttt gagcggtttg tcggggagtt cgttcagcga
     4201 ttgcagatat ttgcgcatcg cgcccgcgca catgccgagc accgcgtcat tgatcgtgcc
     4261 gtcataggct ttgcccatcg ccctgacccg gtccagcgac cagctttgcg cgacgaaacg
     4321 ccgcgcgccg gtgatgttgc ggttgaagct ggtacgcgga accccggcaa aaggcgtcgc
     4381 gaggccgtcg ccgccgatgc cgaacatcgc cgcgagatac tggttcatcg ccttggtgac
     4441 gccgacgcta ttgccccatt gctctttcag gaaatcgccg atcgccttga tgtcggtgag
     4501 gttcgggcgc gcgggtttct ttggcggcgg gaactggcgc ttataggctt cataggcctc
     4561 gaccgagagc ggcgaggcgg cgcgccgttc ctcggggtcc gccgacagca tcgaattgta
     4621 gaaatggatg ctcgcggcgc catccatcag cgcgtggtgc acctttttga aggtcgcgat
     4681 ctgccggttc ggcaggcccg agatcagcgt gatttcccac aagggacgcg tgcggtcgag
     4741 caggcctgag tgcagccgcg aggcaagctc gaacatctcg cgatagcgcc ccggcttggg
     4801 aagcgccgcc gcgcgaacat gatagtgcat gtcgatgtcg cggtcggggg tgaggcggat
     4861 cgggccatat tggccgaagg gggtcagttt cagcacttcg ctgaacggcc gcctgaggcc
     4921 ttccgactgg cggagcagtg caagctgctc gttcagccac tcggtttcgt cgaccccgtc
     4981 gggcagcgtg tagagattga tgccgccgat gtgcatcggc atctcgcggc tctcccccca
     5041 caggaacatc gcgtcggtca ccggcatcaa ccgcatcggc agctctcccc actcaaaatg
     5101 ctgcatcttt tgaggcgcag catgacaggg ggcgtaggcg agtcaagctg cccttcgctt
     5161 ccggggtgac gaaggtggag atgcacggta tcgcaaccga tatggccaag ctctatttct
     5221 attatgccag catgaacgcg ggcaaatcga cgacgctgct gcaggcggat ttcaactatc
     5281 gcgagcgcgg catggagacg atgctatgga ccgcggcgct cgacgatcgt tatggcgcgg
     5341 gacagatcac cagccgcatc ggcctgcttg ccgaggcgca taaattcgat cccgacagcg
     5401 acatgttcgc ggctgtgacc ggagaaaacc ggcagcgccc gctcgcctgc gtgctggtcg
     5461 acgaggcgca attcctgtcg cgcgaacagg tgctgcaatt ggcgcggctc gccgacgagg
     5521 cggatattcc ggtgctctgc tatggtctgc gcaccgattt cgcggcgaac ctctttcccg
     5581 gatctgcggc gctgctgggg ctggccgacg cgctcgtcga gctgaaggcg gtgtgcgagt
     5641 gcgggcgaaa ggcgacgatg aacctgcgcg tcgacgaaaa gggccgcgcg gtggtcgagg
     5701 gtgcgcagac cgagatcggc ggcaacgacc gctatgtcgc gatgtgccgg cgccacttca
     5761 tggcgaaacg gcgcgaagcc gcggaggagc ttgccgatgc ttgaccgtct ttacaccgaa
     5821 ctcgccgacg gcgacctcag gctcgtcaaa ctggccgagc ctcaccgcga agcgttgcgc
     5881 gccgcgtgcg ccgccgatgc cgacgtgtgg ccgatctatt cgtcgagctt cgatccggag
     5941 catttcgacc agagcttcga cacgctggtc ggcggcaagt tccgcatgcc ctatgcgatc
     6001 ttcgacggcg acacgctcgt gggcatgacc gcctggctgc gccccgacat gtcggcgcag
     6061 acggtcgaga tcggcaatag ctatatccat cccgccgcgc ggggcaccgg cctcaacggg
     6121 cggctcaaga aactgatgct cgaccacgcc ttcgcggtcg gcatccgccg catcgaattc
     6181 cgcatcgacg aacgcaacaa gcgcagccag gcggcggtcg cgaagctcgg ctgcacgaag
     6241 gaaggcgtgc tgaggtccga gcgcatcacc tggaccggct atttgcgcga caccggactc
     6301 tggtcgctgc tcgcggacga atggcgacgc tgaatcgtag cgccggggtc agcacgggtt
     6361 cagactcgat ctgctatgca cccgtcatta tgaaaccgaa actcgctctc tccctggccg
     6421 tcctgctgct tcccctttct gcggcggcgg ccatcgagcc tgccgaagaa ccgcgtgcgc
     6481 tcggcgtgga atcgagcatc gtctttccca gcgacagttc gatccgcaac tggaaggccg
     6541 accgcggccg cggcatctgg atcgagggcc gccgcggcga atggtattat ggcaccttcg
     6601 ccggcatctg ccgcgacctc gatttcgcgc aggcgatcgg tgtcgaaacg cgtggtgccg
     6661 ggcggctcga caaattcgcg tcgatcatcg tgcgcggcga acggtgcatg ctctcctcct
     6721 tcgtgacctc ggcgccgccg ccgtcgaagc gcgacaaggc gaaggacatc atgggcgaaa
     6781 agcccgcagc aaattgacaa ttgctgcccg gcgcgcctag cggcgctgcg atgaacggct
     6841 ggatcattct cgacaaaccc gtggggctcg gttcgacgca ggcggtcggc gcggtcaagc
     6901 gcgtgtgccg cgaggcgggg ctgggcaagg tcaaggtcgg ccatggcggg acgctcgacc
     6961 cgctcgcgtc gggggtgctg ccgatcgcgc tcggcgaggc aaccaaactc tgcgggcgga
     7021 tgctcgacgc gagcaagatt tacgacttca cggtgcagtt cggtaccgag accgcggggc
     7081 tcgacgccga gggcgaggtc gtggcgacga gcgacgtgcg gccgacgctt gcggaaatcg
     7141 aagcggtgct gccgcgtttc accgggccga tcgaacaggt gccgccggct tattcggcga
     7201 tcaaggtggg cggccagcgc gcctatgacc tcgcgcgcaa gggcgaggtc gtcgagatga
     7261 aggcgcgcgc ggtcacggtt tactcgttaa agcccctccc cttcagggga ggggttgggg
     7321 tgggggccgc cggagtgcgc ggcgccgatg cccaccccgc tgcgactagc gagcaggctc
     7381 gcaagtctcg ctgcccctcc cctgaagggg aggggctaaa tgagatcacc atcaccgcgc
     7441 atgtctcgaa ggggacctat atccgcagcc tcgcacgcga catcgcgcgc gcggtcggca
     7501 gcgtcgggca tgtcacgatg ctccgccgca cgcgagccgg gcccttcacg ctcgaacagg
     7561 cgatttcgct ggacaaattg aacgctttcg gccaaggggc cgcgcaatca gaaataattc
     7621 tgccgctcga ggcagggctg gtcgacatcc cggctctgaa cctttccccg gaagcggcag
     7681 gggcgatccg tcagggtcgt gtctggaccg ggggtagcac ggatgacggg ctctattggg
     7741 gaagggacag cgacatgcgt cccgtcgccc tgatcgaggc tttggcggga acgctgaaag
     7801 tcgtccgggg cttcaatctg taaacaagga tgttcgagga aaactattta tgtcgattac
     7861 cgccgagcgc aaagccgaag tcatcaagga caatgcccgc gaaaagggcg ataccggttc
     7921 gccggaagtc caggtcgcga tcctgaccga ccggatcaac acgctgaccg atcacttcaa
     7981 ggcgcaccac aaggacaacc acagccgccg cggcctcctc atgatggtca acaagcgccg
     8041 cagcctcctc gactatctca agaagaagga cgaggctcgc tattcggcac tgatcgcgaa
     8101 gctgggtctc cgcaaataag agtttctggc ggccccggat ttccggggcc gctgtcatat
     8161 tggtcccgtg cgatcgtgcc gggccgcagg gccgttcccg catccggcgg ggccgccata
     8221 gggcagacag acgccctgaa ccgtcccggg ccggactgcc cggaatcaga ggccccgccc
     8281 ggcatagggc ccggcgggcg aaggaaacca catgtttgac gtgaaaaaag tatcgatcga
     8341 gtggggcggt gaaacgctga cgctcgaaac gggcaaggtt gcccgccagg ccgacggtgc
     8401 cgtgatggcg acgctgggcg aaacggtcgt cctgtgcgcc gtgaccgccg ccaagtcggt
     8461 gaaggacggg caggacttct tcccgctcac cgtccattat caggaaaaat attcggccgc
     8521 cgggcgcatc ccgggcggct tcttcaagcg cgaacgcggc gcgaccgaaa aggaaacgct
     8581 cgtcagccgc ctgatcgacc gtccgatccg cccgctgttc cccgaaggtt tctacaacga
     8641 gatcaacgcc atcgctcagg ttctgagcta tgacggccat aacgagcccg acatcctcgc
     8701 gatggtcgcc gcgtcggccg cgctcaccat ttcgggcgtg cccttcatgg gtccgatcgg
     8761 cgccgcgcgc gtcggctata tcgatggcga atatatcctg aacccgaccg acgcgcaggt
     8821 tgccgaaggc gacctcgacc tcgtcgtcgc cgccacttac gatgcggtga tgatggtcga
     8881 atccgaagcc aatgagctct cggaagaagt catgctcggc gccgtgctct tcgcgcacga
     8941 cgcgtgcaag gaagtcgtca aggcgatcgt caagctcgcc gaacaggctg ccaaggatcc
     9001 gtgggacatg gccgcgtcgg acgacaacgc cggcatcaag gacaagctca agaagctcgt
     9061 cggcaaggac atcacggccg cctataagct gaccgacaag tcggcccgct cgaacgcgct
     9121 caacgaagcg cgcgcgaagg cgaaggaaag cttcgctgcc gatggcctca gcccgcagga
     9181 agtcatggcc ggcatcaagc tcgtcaagaa gctcgaagcc gaaatcgttc gcggcgccat
     9241 cctcaaggac ggcaagcgca tcgacggccg cacgacgacc cagatccgtc cgatcgtcgc
     9301 cgaaacgcac ttcctgccgc gcgcgcacgg ctcggcgctg ttcacccgcg gcgagacgca
     9361 gacgatcgcg acctgtaccc ttggcaccaa ggatgcggag cagatgatcg acggcctcaa
     9421 tggcctgtcg taccagaact tcatgctgca ctataacttc ccgccctatt cggtcggcga
     9481 agtcggccgc ttcggcgccc cgggccgccg cgaagtcggt catggcaagc tggcatggcg
     9541 tgcgctgcac cccgtgctgc cgaccaagga cgagttcccc tatacgatcc gcctcaccag
     9601 cgacatcacc gagtcgaacg gctcgtcgtc gatggcgtcg gtttgcggcg gcagcctcgc
     9661 gatgatggac gccggcgtgc cgatcaagcg ccccgtttcg ggcatcgcga tgggcctgat
     9721 cctcgaaggc aaggattatg cgatcctgtc ggacatcctt ggcgatgaag atcacctcgg
     9781 cgacatggac ttcaaggttg cgggcaccgc cgacggcatc accacgatgc aaatggacat
     9841 caagatcgcg ggcatcacgc gcgagatctt cgaagccgct ttgaaccagg cgaaggaagg
     9901 acgcgcgcac atcctcggcg aaatggcgaa ggcgctgggc gaagcccgca ccgagctgtc
     9961 ggcgcacgcg ccgcgcatcg aaacgatgca gatcgacaag tcgaagatcc gcgacgtcat
    10021 cggcaccggc ggcaaggtga tccgcgagat cgtcgcgacc accggcgcca aggtcgacat
    10081 cgacgacgaa ggcttgatca agatctcgtc gagcgacctc gaccagatcg aggccgcgcg
    10141 caagtggatc agcggcatcg tcgaggaagc cgaagtcggc aagatctatg acggcaaggt
    10201 cgtcaacctc gtcgatttcg gtgcgttcgt gaatttcatg ggcggcaagg acggtctcgt
    10261 ccacgtcagc gaaatcgcca acgagcgcgt cgagaaggtc gccgacgtcc tcagcgaagg
    10321 tcaggaagtc aaggtcaagg tcctcgagat cgacccgcgc ggcaaggttc gcctgtcgat
    10381 gcgcgtcgtg aaccaggaaa ccggcgagga actcgaagac acccgcccgc cgcgcgaacc
    10441 ccgcggtgat cgcggcccgc gccgtgacgg tggcgac
//
LOCUS       DATMSU010000070        12514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172062,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000070 DATMSU010000000
VERSION     DATMSU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 12514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12514
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172062"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1097
                     /locus_tag="VN034_01800"
     CDS             69..1097
                     /locus_tag="VN034_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HWV59358.1"
                     /translation="MEKIATVGLDIAKAVFQVHGVDAAGEVLVRKRLSRARVIPFFAR
                     LPRCLVGIEACSTSHHWARELIALGHDVRLMPAQYVKPYVKRGKNDAADAEAICEAVT
                     RPTMRFVTVKTPEQQSVMMLHRVRLMLNRQRTQLSNATRAHMSEFGIVAPVGRIGLER
                     LLAIVADKDDETIPPDARLCLEMLGAQLAIVKEQILENDRRIRASARDTEVGRRLMEI
                     PGIGPLLASAFVASVADPRLFKTGRDLAAWIGLVPRQNSSGGKERLGGISRAGNQYLR
                     QMLVVGSMAVIRHAERHGTKRPWLIELMKRRAKKVAAVALANKTARTVWALMTSGEHY
                     REPQVAHA"
     gene            1537..2235
                     /locus_tag="VN034_01805"
     CDS             1537..2235
                     /locus_tag="VN034_01805"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV59359.1"
                     /translation="MGENITISIDGGSLGAFVERPASGSGPVVVVLHEVFGVNDDMRA
                     SCRELAEQGFIALAPDLFWRQEKGVDLSHWTDEEWKKGLALYQAYDRAQGAIDIAAIM
                     EFAGSLPGAAGKVGLMGYCLGGLMTYLVSARHGADAGVAYYPGSAEDYASEAKDIRSP
                     LMIHLGEEDEFISKAAQQTIKSAVAQNPSVVIFSYPGCSHAFARHTGTHYDADAAALA
                     NGRTYAFLKERLEG"
     gene            complement(2445..2828)
                     /locus_tag="VN034_01810"
     CDS             complement(2445..2828)
                     /locus_tag="VN034_01810"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWV59360.1"
                     /translation="MGAPNDVDRFFAALTHSLKAEMERVRDIIMSASPLLTETVKWGG
                     PSFEYGETLATFSPRVKDCVALVFHDGAPFLDRFAFLEAGPKGKAYAKFRSMDDIERD
                     RTDLSALVGEFVAKMDDDLRCNAKV"
     gene            complement(2882..3424)
                     /locus_tag="VN034_01815"
     CDS             complement(2882..3424)
                     /locus_tag="VN034_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWV59361.1"
                     /translation="MKREYRKGAYLVTQGQRTRNIFYVVQGLLLQYHLSPDGDAIVKR
                     FFPEGSFAASTSALLTERDSDFSIKALEPVVVWEYDFSSFKALVQHHPDIAAFYIRYM
                     ERHWIVEKEPLEISFRNEDARAKYARFLKTYPGLETRIRQHEIAAFLGVTPTQLSRIR
                     ADRRQDGASTNVNAAPRPAS"
     gene            3757..4113
                     /locus_tag="VN034_01820"
     CDS             3757..4113
                     /locus_tag="VN034_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59362.1"
                     /translation="MTAEKELYEIEEGFWLKGQEHFLDHVDERCLLAFPQMGEMHGVH
                     SRAEVAATATSTNRWRDLVMKNRQFLDGGEDFKIISYRAEVTRGDGEPYAALVSSAYV
                     RRPDGWKLAFHQHSPV"
     gene            complement(4144..4554)
                     /locus_tag="VN034_01825"
     CDS             complement(4144..4554)
                     /locus_tag="VN034_01825"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HWV59363.1"
                     /translation="MKAVFTFQALLCAAFLSSAFSADASSAKTGPNDAARNWSVSGDE
                     LIDALKRRPNSGASVGSTDRARSPAEGAAYIAGVADATGASSWCGAGSVLPHEFVDRV
                     YTYLRTVPPERLKASASTLVIEALADSFPCAADK"
     gene            complement(4605..5426)
                     /locus_tag="VN034_01830"
     CDS             complement(4605..5426)
                     /locus_tag="VN034_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HWV59364.1"
                     /translation="MTMFSHASWSGSEFLALYAMLLMAVIYLGFAIPHSLRPGGREAR
                     SLPDEQVAYLAGGRERYAELVATRLMTADAITITADRKVAIQGTNGLHGPGEAAILAL
                     PSPTDWSAIDHALRASAENVRKSLIAGGLLMRRPRVILIRLCQTLPYLLLIALGLSRW
                     RYGNALDHPTGFLTALMILTAFLALLRWFTLEKRTRSGFAAIEDAQARAERLRRAPMS
                     DEIPLAVALFGTAVLAGSNLTDFHQMRTDSNGSSDGGGGCGGGGGGCGGCGGCGG"
     gene            complement(5423..5962)
                     /locus_tag="VN034_01835"
     CDS             complement(5423..5962)
                     /locus_tag="VN034_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59365.1"
                     /translation="MWHALWSYEVGPEKADLTFTARLARENGWAEAYAERVFDEYRCF
                     CFLAATSDAELTPSDAVDQVWHLHLTYTRDYWERFCPKVLGRPLHHGPTAGGSDERSR
                     FFDQYARTLRRYEQVFGAPPPDDIWPDAARRLIDDPRARRVHPRDGVVLPRSALRGAS
                     IIAACLLTGVGLWFGSVVL"
     gene            complement(6136..6462)
                     /locus_tag="VN034_01840"
     CDS             complement(6136..6462)
                     /locus_tag="VN034_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HWV59366.1"
                     /translation="MGGTKRRKPRDRRVRVLEYDTRPTAVMGPLGPLTIDDLPSSGTI
                     YWVGRRKAEVLAAIDGGLLSLEEACDRYRLSNEEIGSWRRSLDRSGLPGLRITKLQRY
                     RGRHMD"
     gene            complement(6622..6972)
                     /locus_tag="VN034_01845"
     CDS             complement(6622..6972)
                     /locus_tag="VN034_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59367.1"
                     /translation="MRVNVDHYVDASDPDADGLHDWYYEYDIYRFSQAGRSFVARAYT
                     DEPRKVAFLAHEVKILGFRRHRLLNSADLRSSLFVAALAHFRRLGMTSIDRLTEAGYV
                     PVENAAPDGVIQNL"
     gene            7364..>12514
                     /locus_tag="VN034_01850"
     CDS             7364..>12514
                     /locus_tag="VN034_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HWV59368.1"
                     /translation="MMGQFSTRANGTRASRSLGKLFASTAVVACAMGLVSGIPLASAQ
                     SVTTSGDVDPSPATSPNWNVGGVLNVGDSATGSLTVAGGGTVTSTFGQVGVNSGGDGT
                     VTVTGAGSNWTSNSSIYVGMFGSGTLNIANGGSVDVDTNSALGEYAGSSGIATVSGPG
                     SRWTTDNFLYVGAAGNGTLGIENGGSVSAAEAIVGEDAGSVGLVTIAGTGSSLSLGSN
                     LVVGQTGAGALEIAGGGAVTTGNQGIIGYFAGSGGTVTVDGPNSVWNLSNALQIGREG
                     SGELTISNGGIVSTAAAPVSLDFRTIGSQQGASGEVTVTGAGSAWLGDANLTVGGEGT
                     GTLTVAAGGLATSGTAVIGSSVDSVGTVTVTGGNSAWTNDDQMFVGFSGKGTMTISKG
                     GTVGNTTAFIGYGAGSDGAVTVTGANSSLTNSADLLVGYAGNGTLNIADGGAVTNAVG
                     AIGYAAGFSGRATVTGPASSWTNLNELFVGTFGGGLLDITDQGSVSSTAGFIGRNAGS
                     NGAVTVTGPGSAWTSSSTIYVGNAGSGTLTVTDGGSVTASQITIAAVAGSTGILNIGS
                     DAGQAATAAGTLDTSGIAFGAGAGSLIFNHTDADYDFDVAITGAGTIQQVAGVTRLTA
                     DNSGFTGSTAISGGTLLVNSTLGDAASEVVVLGGGTLGGNGTIGGNVTVGGGATLAPG
                     NSPGTLTVGGDLELTPNAVLAFEFGRAGIIGGALNDLVNVGGDLTLAGTIDVLVPTGG
                     SFGPGIYRMFNYGGTLTDNGLTLGTMPGGSAVSVQTAVTGQVNLVNTAGLTLSFWDGN
                     AGPKNDGTVNGGNGVWRVGGGSSNWTDAGGALNADYAQDTFAIFSGAPGDVTVDDAGG
                     DVLASGLQFASSGYRVAGDALTLTDAQAIVQVGDGSAAGAGFAATIASELAGTAGLVK
                     TDAGTLVLTGANSYTGGTAIDGGTLQIGSGGASGSIIGDIDNEGALVVDRSGALTLDG
                     TISGSGSLTKRGSGTLTLTGTNSYAGGTTINGGTIQISNDANLGDVGGTLSLQIGTLQ
                     AGAAFTSARNILVGGAGANRIDTQGFDVTLSGIVSDGPNSSFGNFFAKLGSGTLTLTG
                     ANSYSTRTVLENGTLALAGAGTIGRGNIIVGAGTVLDISQGDVGARVIQLNRGPAGTI
                     ALGSKILTLGFNNSFSDWSGTITDGGIAGGTGGRVVITGSRGAVRYLEANSYTGGTTV
                     SAGSFELFGNGSLYAGGAVAVGAGAAFGIAGLNGPGTTIGDLSGAGAIALGAKTLTFG
                     TGADTAFSGTISGAGGGLVKTGTGTFTLTGANNYTGSTSVTAGALLVNGDQSAAIGLT
                     SVSSGAVLGGTGTIGGDVVIADGATLAPGAGGAGALTINGDLSLGATSQLAFEFGRAN
                     APGDPLNDVVNVGGDLTLGGTLDVAVAPGGAFGIGVYRVANYGGTLINNGLALGTMPS
                     GADLFVQTSVANQVNLINTGGAVLNFWDGSAGANKFNNVVDGGSGMWRTGLDNSWTET
                     TGAANAGYANGAFAIFAGTGGIVTIDNSLGAVTASGLQFATTNYRVQGGALTLTGPQS
                     VIRVGDGTSAGLGYSTIITSEITGGAQLVKTDVGRLELTGTNSYTGGTALNGGTVRIS
                     SDANLGAGSGVLSFNGGTLETTADISSVRTVDLAGQGVFSIDADTALTLTGNLTGAGL
                     IGKLGAGTLVLAGAGNHAGGIGVAQGTLLVNGDYGAATGPVTVNQFTTL"
BASE COUNT         2343 a         3865 c         4068 g         2238 t
ORIGIN      
        1 tgatgacgtg gacggctctc caacttcccg cggtatgatc cgcaggatga gctggaagga
       61 gagctacgat ggagaagatt gcaacagtcg ggttggatat cgcgaaggcg gtgtttcagg
      121 tccacggtgt tgatgccgca ggcgaggtcc tggtgcgcaa gcgcctgagc cgggcgcggg
      181 taattccgtt cttcgcgagg ctaccgcgat gtctggtcgg catcgaggcc tgcagtacat
      241 cgcatcattg ggcccgcgag ttgatcgcgc tggggcatga tgttcggttg atgccggcgc
      301 aatatgtgaa gccctatgtg aagcgcggca agaatgacgc tgccgatgcc gaggcgatct
      361 gcgaggcggt gacgcggccg acgatgcggt tcgtgacggt gaagactccg gagcagcaga
      421 gcgtgatgat gcttcaccgg gtccgactga tgctcaaccg ccagcgcacg cagctgtcta
      481 acgcgacgcg ggcccatatg tcagagtttg ggatcgtggc gccggtgggg cgcattgggc
      541 tcgagcgcct gctggcgata gtcgccgaca aagatgacga gacgattcca cccgatgccc
      601 ggctctgcct ggagatgctc ggggcgcagc tcgccatcgt gaaggaacag atcctcgaga
      661 acgaccggcg catccgtgcg agtgcgcgcg acacagaagt cgggcggcgg ttgatggaga
      721 ttcctggcat cggcccgctg cttgccagcg cctttgttgc cagcgtcgcc gatccgcgtc
      781 tgttcaagac aggccgcgac ctcgccgcgt ggataggctt ggtcccgcgg cagaactcca
      841 gcggggggaa ggagcggctc ggcggtatat cgcgcgcggg caaccaatat ctgcgccaga
      901 tgctcgtcgt cggttcgatg gccgtcatcc gccatgctga acgacacggc accaaacggc
      961 cgtggctcat cgaactgatg aagcgtcgcg ctaagaaggt cgcagctgtg gcgctagcga
     1021 acaaaaccgc ccgcacggtc tgggcgctga tgacgagcgg cgagcactat cgagagccac
     1081 aggtcgcgca cgcataggag atcaggcgcc gaacatggcg tcgccgaggt tgggaagggc
     1141 agacaggagc tgatgcacaa agccggttga aaccgccgga tcaggaaaac ccattttggc
     1201 cggtgcacct cgagtgcgtc caaatggtcg ggacccgatc cgcgcgaaag gcatcatggc
     1261 cagcggcgca tgacaaggcc gcatcacagg ccggacacat ggctgcacca accagcaact
     1321 gcatcatcca gaaaccgctt gccaacggag ggccgtccac acacggccaa tacctgcgga
     1381 aatcttctgc acaaaagcaa aaagccgctt gcagatcatc tcgagcaatc gcagtctttg
     1441 cggcgcattc aggccggatc cgggtcgaac tccgtgtagg ctggcatgcg atcacgatat
     1501 ttactgggac cgacgacagt cagtgggaga accgagatgg gggaaaatat cacgatctca
     1561 attgacggcg gctcgctcgg cgcgtttgtc gaacggccgg cgtctggaag cggacctgtc
     1621 gtcgttgttc tgcacgaagt cttcggcgtg aacgacgaca tgcgagccag ctgccgcgaa
     1681 cttgcagagc agggcttcat tgcgctcgca cccgatctct tctggcgaca ggaaaaaggc
     1741 gtcgacctct cacactggac tgacgaggaa tggaagaagg gcctcgcgct ttatcaggcc
     1801 tatgatcgtg cgcagggcgc gatcgacatc gccgctatta tggaatttgc cggttcgctt
     1861 ccgggtgccg ccggcaaggt gggattgatg ggatattgtc tcggtggatt gatgacctac
     1921 ctcgtgtccg cacggcatgg cgccgatgcg ggcgttgcat attatcccgg ctccgcggag
     1981 gactatgctt cggaggccaa ggacattcgc agtcccctga tgattcatct cggcgaggaa
     2041 gacgagttca tctcgaaggc agcgcagcaa acgatcaagt cggcggtcgc acagaatccc
     2101 tcggtcgtca tcttctctta ccccggctgc agccatgcgt ttgctcggca cactggcacc
     2161 cactatgacg ccgacgcggc cgcgctagcg aatgggcgca cctacgcgtt cctcaaagag
     2221 aggctcgagg gatagcagga gagctggctg cacccgggag gcggcgtgac cggcgaatcg
     2281 ggagaggatt tttcgtctct accgggtcgc cgtcgatatt cgttcgacgc cgatgaagct
     2341 caatttcagg ggcgccgaga acggctgctt tcgctggata tctggttaaa cctgcctgcc
     2401 cgccttcggc caacctaagc caaatcatct ctttgcaata atcattaaac cttcgcattg
     2461 caccgtaggt cgtcatccat cttcgcgaca aattcgccga cgagtgcgct cagatcggtg
     2521 cgatcccgct cgatgtcgtc catcgaacgg aatttcgcgt aagccttgcc cttcggacct
     2581 gcttccagaa aggcgaagcg atcgaggaac ggtgcgccgt catggaagac gagcgccacg
     2641 caatctttca cccgcgggct gaatgtcgcc aatgtctcac catattcaaa actcgggccg
     2701 ccccatttga ctgtctcagt caataaaggg ctcgcgctca tgatgatgtc gcgtaccctc
     2761 tccatttccg ccttcaacga atgcgtgaga gccgcgaaaa acctgtccac gtcgttcggt
     2821 gcgcccatgt tcatctcctg cgctatgccc tgtccggcaa ccatgccgtt gcaggtgaag
     2881 cctacgatgc cggacgcggc gccgcattta catttgtcga tgcgccatcc tggcggcggt
     2941 cggcgcggat ccggctcaac tgggtcggcg ttacgccgag aaaggcggca atttcgtgct
     3001 gcctgatccg tgtctcgagg cccggatatg ttttgagaaa gcgcgcatat ttcgcgcgcg
     3061 catcctcgtt ccggaacgat atctccaagg gttccttctc gacgatccag tggcgttcca
     3121 tgtaccggat atagaaggcg gcaatatcgg gatgatgctg cacaagcgcc ttgaagctcg
     3181 agaaatcata ttcccagacg accaccggct cgagcgcctt tatggaaaag tcgctgtccc
     3241 tttcggtcag cagggcgctg gtcgatgcgg cgaaactgcc ctcagggaag aagcgcttca
     3301 cgatcgcgtc gccgtcgggg ctgaggtggt attgcagcaa caggccctgt acgacgtaga
     3361 agatgttgcg cgtccgttgt ccctgcgtga cgagatacgc gcccttgcga tattcgcgtt
     3421 tcacaaggag gcggcgccac gcgtcccgtg cgccctcatc gaggcgggaa tattccgcga
     3481 ccttgcgaaa aaaacgcgtc cgctgcctgc aacatcggct ccgtcaactg cttgcctgac
     3541 cgtgtcggac gtaaccgcct tggctgcgct gatcaacgcc gcggcaatag gccgaaaaat
     3601 acggtcctgc attctcggct ttgctcctga agcttcacca tgggacgtcg gtcctggtcg
     3661 gtcgcattac gacccgggcg cttcgtgcga tccgtcctcc gccagatgca acccgcagcg
     3721 cgccgtgcgg gtttcggatc gcgaaaggat agaacgatga cggccgaaaa ggagctatac
     3781 gagatcgagg aaggcttctg gctcaaggga caagagcatt ttctcgatca tgtcgacgag
     3841 cgatgcctgc tcgccttccc gcagatggga gagatgcacg gcgtacattc gcgcgccgaa
     3901 gtcgcggcga cggccaccag caccaaccgc tggcgcgacc tcgtgatgaa aaaccgccag
     3961 ttcctcgacg gcggcgaaga tttcaagatc ataagctacc gcgcggaagt gacgcgcggc
     4021 gatggcgagc cctatgcggc gctcgtgagc tcggcttatg tacggcgacc cgatggctgg
     4081 aaactcgcct ttcaccagca ttctcccgtt tgaccgctgg cggcctggac tgccccgaag
     4141 cgttcatttg tcggcggcgc aagggaagct gtcggcaagc gcctcgatca ccagcgtcga
     4201 tgcgctcgct ttgagccgct ctggcggaac ggtgcggaga tatgtgtaga cgcggtcgac
     4261 aaactcatgg ggaagaaccg atcctgcccc gcaccagctg cttgcgcccg tggcatccgc
     4321 tacgccagcg atataggccg cgccttcggc gggtgacctc gcacggtcgg tgctaccgac
     4381 cgaagcgccc gaattcggcc ttcttttcag cgcgtcgatc agttcgtctc cgctgacgct
     4441 ccagttgcgg gccgcgtcgt tcggtccggt tttggccgat gaagcgtcgg cactaaaagc
     4501 cgacgacaga aaagccgcac acaggagcgc ctggaatgta aatactgctt tcatctttta
     4561 ctccgttgat aaggcagcgt gcgatcgaac gcattcgtcg tcattcagcc cccgcagccc
     4621 ccgcacccgc cgcatccacc cccgccgccg ccacagccgc cgccgccatc gctcgagccg
     4681 ttgctgtcgg tacgcatctg atgaaaatcg gtgagattcg aaccagcgag cacggcggtg
     4741 ccgaacagcg ccacggcaag gggaatttca tccgacatcg gggcgcggcg gagacgctcg
     4801 gcacgcgcct gtgcgtcctc gatcgccgcg aagccgctcc gggttcgctt ttccagcgtg
     4861 aaccagcgca gcagcgcgag aaaggcggtc agaatcatca acgccgtcag aaagccggtc
     4921 ggatgatcga gtgcgttgcc gtagcgccag cgcgacaggc caagcgcgat gaggagcaga
     4981 tagggcagcg tctggcaaag gcggatcagg ataacgcgcg gccgccgcat gagcagcccc
     5041 ccggcgatca ggctcttgcg gacgttctcg gcgctggcgc gaagcgcatg gtcgatcgcg
     5101 ctccagtccg ttggtgaggg gagggcaagg atcgccgctt caccggggcc gtgtaggcca
     5161 ttcgtgccct ggattgcgac ttttcggtcc gctgttatcg tgatcgcatc tgcggtcata
     5221 agccgcgtcg cgacaagctc ggcataacgc tcgcgcccgc ctgccagata ggcgacctgt
     5281 tcgtccggga gagatcgcgc ttcccggccg ccggggcgga gagaatgcgg aatggcaaag
     5341 ccgagataga tgacggccat aagaagcatg gcataaagcg cgaggaactc gctgccgctc
     5401 cagcttgcgt ggctgaacat tgtcataaga cgacactccc gaaccatagg ccaacaccgg
     5461 tgagaaggca ggctgcaatg atggatgccc cgcggagcgc gcttcgcggc agcacgaccc
     5521 catcgcgcgg atggacgcgg cgtgcgcggg gatcgtcgat cagacgccgc gccgcatcgg
     5581 gccagatatc gtccggcggc ggcgcgccga aaacctgttc gtatcgacgc agcgtccggg
     5641 catattggtc gaagaagcgt gagcgttcgt cgctgccgcc ggcggttggg ccgtggtgca
     5701 ggggacggcc gagcaccttg gggcaaaatc gctcccaata gtcgcgcgta taggtgaggt
     5761 ggaggtgcca gacctgatcg acggcgtcgg acggcgtcag ctcggcgtcg ctcgtggcgg
     5821 cgaggaagca gaaacaccga tactcgtcga acacgcgttc cgcgtatgct tcggcccagc
     5881 cattctcgcg cgcgaggcgc gccgtgaacg tcagatccgc tttctcgggg ccgacctcat
     5941 aggaccaaag cgcgtgccac accggatggt ccgggctgat cggtcgggcg tgatccggcg
     6001 ctggcatcag aactgaaata tatcatgggc ttgcgcgtgc ataaagcgaa tttggaagga
     6061 cggcgcgagc gggaccagtc gcccgcgccg tccggccggt gccgcgcatg gtgggggtag
     6121 cgcgacatcg atcgatcaat ccatatgtcg tcctcgatac cgctggagct tggtgatgcg
     6181 aagcccggga agtccggagc gatcgagcga gcgccgccac gagccgattt cttcattgct
     6241 cagcctgtat ctgtcgcacg cctcctcgag actgagcaat ccgccgtcga tcgcagccag
     6301 gacctcggcc tttcggcggc cgacccaata gatcgtgccg gacgacggca gatcgtcgat
     6361 cgtcaatggc ccgagaggac ccatgaccgc cgtcggcctc gtatcatatt cgagcacgcg
     6421 aactctccga tcgcgcggct ttcgccgctt cgtgcccccc atcggaagtc tgctcgaaat
     6481 gcgttaacaa aatctttccg tgacgcgcga cccgatccgt ccggccgtca cgcgccgact
     6541 tttcatctgg agattactcg cccggagccg gcctttcccc gacgctgcat gacggccctt
     6601 tcgcgtcgtt gcggcaacgt cctagagatt ctggattacg ccgtcagggg ctgcattttc
     6661 aaccggcaca tagccggcct ccgtcaaccg gtcgattgag gtcatgccga ggcggcgaaa
     6721 gtgagcgagc gctgccacga acagcgacga tcggagatcc gcggagttga gaagcctgtg
     6781 tcggcgaaag cccaaaatct ttacctcgtg cgcgaggaag gccaccttcc tcggctcgtc
     6841 cgtataagct cgggcgacga agcttctgcc ggcctgcgag aaccggtaga tgtcatactc
     6901 atagtaccag tcgtgcaagc catctgcatc ggggtcggag gcgtcaacat aatgatcgac
     6961 gttgacgcgc atgtctattc aatggccggg caaatgcggc atgacaaccg gtcattttcg
     7021 gtgtgttgtc tgtggctcca acatgcattg tacgcttcca ccaccgcacg caatcacaag
     7081 gccgtttcag gaatgtgtcc cgctcaggtt actcgaacgc ctaaagcagc cggaccgcta
     7141 gcggccatct gttcgcgacg cccttgatga taacagaggc tcaacctcaa tccttcgagc
     7201 acgtgcagag ccgggatggg gcccttgtcc cgtatcggct caaagatgtc gaaacaactt
     7261 gggcgtgtca ggtgtgcgta ccttttttgc ataaaattga agatgttgtg attgccccgc
     7321 gcgtcttgcg cccttaaccg gtccgtcaaa cagaggaggc ggcatgatgg ggcaattctc
     7381 aacgcgcgca aatggaacgc gcgcttctcg cagtcttggg aaactcttcg cctccacggc
     7441 ggtcgtcgcc tgcgcgatgg ggctggtctc cggcattcct cttgcatcgg cccagagcgt
     7501 gacgacatcg ggcgacgtcg atccttcccc cgccacctca ccgaactgga acgtcggcgg
     7561 ggtcctcaat gttggcgact cagcgaccgg tagcctgacg gtggcgggcg gtggaacggt
     7621 cacgagcacc tttggacagg ttggtgtcaa tagcggcggc gacggaacgg tgacggtgac
     7681 gggggcgggc tcgaactgga cgagtaacag cagcatctac gtcggcatgt tcggctccgg
     7741 aacgctgaac atcgcgaacg gcggcagcgt cgacgtcgac accaatagcg cgctcggcga
     7801 atatgccggt tcctcgggga ttgcgacagt gagcggtccg ggctcgcgct ggacaaccga
     7861 caatttcctt tatgtgggcg cagcgggcaa tggaacgctg ggcatcgaaa atggcggctc
     7921 agtgtccgcc gcggaggcca tcgtcggcga agatgccggc agcgtgggtc tggtgacgat
     7981 cgccggaacc ggttcgtccc tcagcctcgg cagcaatttg gtcgtcggcc agaccggtgc
     8041 cggtgcgctg gagatcgccg gcggcggcgc cgtcacgacc ggcaaccagg gcatcatcgg
     8101 atattttgcc ggatcgggcg gcacggtaac cgtcgacggg ccgaattcgg tctggaacct
     8161 cagcaacgcg ctgcagatcg gccgcgaggg aagcggcgag ctcaccatct ccaacggcgg
     8221 tatcgtttcc accgcggcgg cgcccgtcag cctggatttc cgaacgatcg gctcgcagca
     8281 aggcgcgagc ggagaggtga ccgtaaccgg ggccggctcg gcatggctcg gggacgccaa
     8341 tctcacggtg ggcggtgagg gcaccggcac actgacggtc gctgccggcg gtctggccac
     8401 gagcggaaca gccgtcatcg gcagctcggt tgattccgtt ggcacggtga cggtgaccgg
     8461 cggaaattcg gcctggacga acgacgacca gatgttcgtc ggcttctcgg gtaagggcac
     8521 gatgacgatc tcgaaagggg gcacggttgg caatacgacc gcctttatcg gctacggcgc
     8581 cggctcggac ggcgcggtga cggtgacggg ggccaattcg tccttgacga acagcgccga
     8641 tctcctcgtc ggctatgcgg gcaacggcac cttgaacatt gcggatggcg gtgcggtgac
     8701 caacgcggtc ggcgcgatcg gttatgctgc cggtttctct ggccgggcga ccgtgaccgg
     8761 gccggcgtca agctggacaa acctcaacga gcttttcgtc ggcacgttcg gcggcggcct
     8821 tctcgacata acggatcagg gaagcgtttc cagtacggct ggcttcatcg gccgcaacgc
     8881 cgggtcgaac ggtgccgtga cggtgacggg ccccgggtcg gcctggacga gcagctctac
     8941 catctacgtc ggcaatgctg gctcgggcac gctgaccgtc actgacggcg gctctgttac
     9001 ggcgtcgcag attacaatcg ccgccgtggc cggaagcact ggcatcctca acatcggctc
     9061 ggacgccggt caggcggcga cagccgccgg aacgcttgac acttccggga ttgcgttcgg
     9121 tgcgggggca ggatcactga tcttcaacca caccgacgcg gactatgact ttgacgttgc
     9181 gattacgggc gcgggcacga ttcagcaggt ggcgggtgtc acccgcctga ccgcggacaa
     9241 ctcggggttc accggctcga ccgcaatctc gggcggtacg ctgctcgtca acagcacgct
     9301 cggtgacgcg gcaagcgagg tcgtcgtgct cggcggcggc acgctgggcg gcaacggcac
     9361 gatcggcggc aacgtcaccg ttggcggcgg cgccacgctg gcgcccggta acagccctgg
     9421 cacgctgacg gtgggcggcg atctcgagct cacacccaat gcggtgctcg ccttcgagtt
     9481 cggtcgggcg ggtatcatcg gtggcgcgct caatgacctt gtcaatgtcg gcggcgacct
     9541 cacgctcgct ggcacgatcg acgtcctggt gccgaccggc ggcagcttcg gcccgggcat
     9601 ctaccgcatg ttcaactatg gcggtacgct caccgacaac ggcctgacgc tcggcacgat
     9661 gccgggcggc agcgcggtgt cggtgcagac cgcggtcacg ggacaagtca atctggtcaa
     9721 caccgcgggc ctgacgctca gcttctggga cggcaatgcg ggtcccaaga atgacggcac
     9781 cgtcaatggc ggcaacggcg tgtggcgcgt cggcggcggt tccagcaact ggaccgatgc
     9841 gggcggggcg ctgaacgccg attatgcgca ggacacattc gcgatcttct ccggtgcgcc
     9901 cggcgatgtg accgtcgatg acgctggcgg cgacgtcctt gcctccgggc ttcagttcgc
     9961 aagcagcggc tatagagtcg ccggcgatgc gctcacactc accgacgccc aggcgatcgt
    10021 tcaggtaggc gatggaagcg cggcgggcgc gggctttgcc gcgacgatcg cgagcgagct
    10081 ggccggcacc gccggccttg tcaaaaccga cgctggcacc ctcgtcctca ccggcgccaa
    10141 cagctacacc gggggtaccg cgatcgatgg cggcacgctg cagatcggct ctggcggcgc
    10201 gagcggcagc atcatcggcg acatcgacaa tgagggggcg ctggtcgtcg atcgttccgg
    10261 tgcgctcacg ctcgatggca ccatttcggg cagcgggtcg ctcacgaaac gcggtagcgg
    10321 aaccctgacg ctcacgggca ccaacagcta tgccggcgga acgacgatca acgggggtac
    10381 gatacagata tccaatgacg ccaatctcgg cgatgtaggc ggtacgcttt ctctgcagat
    10441 cggcaccttg caggctggcg ctgcgttcac ctctgcacgc aatatcctag tcggcggagc
    10501 cggggccaat cgcatcgaca ctcaaggatt cgacgtaacg ctgagcggca tagtcagtga
    10561 tggtcccaac agctcgtttg gcaatttctt cgctaagctc ggcagcggaa cgctgaccct
    10621 gacaggtgcc aacagctatt ccacccgaac cgtgcttgag aacggcacat tggcattggc
    10681 cggggccggc acgatcggca ggggcaacat catcgtgggc gcaggcactg tcctcgacat
    10741 ctcgcagggc gatgtgggcg cacgcgtgat ccagctcaac aggggccccg caggtacaat
    10801 agcgcttggc tctaaaatac tgacgctcgg attcaacaat tcgttctctg actggtcggg
    10861 gacgatcacc gacggcggca tcgctggcgg caccggtggc cgggttgtca ttacgggctc
    10921 ccgcggcgcg gttcgctacc ttgaggcaaa cagctacact ggtgggacca cggtatcggc
    10981 tggcagcttc gaactgttcg gcaacggctc gctttacgcg ggcggtgcgg tagccgtggg
    11041 cgccggcgcc gcgtttggta tcgccgggct taacgggccc ggcacgacga tcggcgatct
    11101 atcgggtgcg ggggcgatcg cgcttggcgc aaaaacgctg accttcggca cgggcgctga
    11161 cacggccttc tccggaacga tctcaggcgc cggaggcggg ttggtgaaga ccggcaccgg
    11221 tacgttcacg ctgaccggcg ccaacaacta taccgggtcg accagtgtca cagcgggcgc
    11281 tctgctggtc aatggcgatc aatcggcagc gattgggctc accagcgtaa gttcgggagc
    11341 tgtgttgggc gggaccggca cgatcggtgg cgatgtcgtc atcgcagacg gcgctacgct
    11401 ggcgccgggt gccggcgggg caggggctct caccatcaat ggcgacctgt cgctcggcgc
    11461 gacctcgcag ctcgccttcg agttcggccg cgcgaacgcg ccgggcgatc cgctcaacga
    11521 tgtcgtcaac gtcggcggcg atctgacgct cggcgggacg ctcgacgtcg cggtggcgcc
    11581 gggcggcgcc ttcggcatcg gggtctatcg ggtcgccaat tatggcggca cgctcatcaa
    11641 caacggcctc gcgctcggca cgatgccgtc gggcgcggac ctgttcgtcc agacctcggt
    11701 cgcgaaccag gtcaacctga tcaacaccgg cggtgcggtg ctcaacttct gggacggcag
    11761 cgccggtgcg aacaagttca acaatgtggt cgacggcggc agcggcatgt ggcggaccgg
    11821 gttggataac agctggaccg agacgaccgg tgccgcgaac gccggctatg ccaacggcgc
    11881 cttcgcgatc ttcgccggta ccggcggcat tgtcacgatc gacaacagct tgggcgcggt
    11941 tacggcctcg ggactccagt tcgcgacgac gaattaccgg gtccaaggcg gtgcgctgac
    12001 gctgaccggc ccacaatcgg tgatccgcgt cggtgacggt accagtgccg ggctcggata
    12061 cagcacgatc atcacctccg agatcaccgg cggcgcccaa cttgtcaaaa ccgacgtcgg
    12121 caggctcgaa ctgaccggca ccaacagcta caccggtggc acagcgctca acggcggcac
    12181 ggtgcggatc tcgagcgacg ccaatctcgg cgccggctcg ggcgtactca gcttcaacgg
    12241 cggcacgctc gaaaccacgg cagatatcag ctcggtccgc acggtcgatc tcgcgggcca
    12301 aggcgtattc tccatcgacg cggatacggc gctcacgctc accggcaatc tcaccggcgc
    12361 gggtctgatc ggcaagctcg gcgccggtac cctcgtcctt gccggtgccg gtaaccacgc
    12421 cggtggcatc ggggtcgcac agggcacgct gctcgtgaac ggcgactatg gcgccgcgac
    12481 cggccctgtg accgtgaacc agttcacgac gctg
//
LOCUS       DATMSU010000071         7253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_153121,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000071 DATMSU010000000
VERSION     DATMSU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153121"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VN034_01855"
     CDS             <1..229
                     /locus_tag="VN034_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59369.1"
                     /translation="TAAAYRALSASRSSDAGARQKVVGLLSQKQAALARLRKHLRIRA
                     LLEIWLYVHVPLTFALIAALSAHIISVFFYW"
     gene            240..2174
                     /locus_tag="VN034_01860"
     CDS             240..2174
                     /locus_tag="VN034_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HWV59370.1"
                     /translation="MSFILRRIATTKTGKQIVRDQALPGDAITLGRESGNIIHIADLA
                     VNPHHATISSADGRHVRVAALEGLGFDFNGRGVDVADIDSAAGAELRFGGHRLTIARE
                     DEKIILLVERIDELSQSSKDVDEAKAFSLAGVVPGKRMGAWVFGLLMLLAFLIGPIWA
                     WYSYKNVNERPDGYHADSAWLSGPLSSAHASLKDDCQSCHVEPFVAVTDKACVNCHTG
                     EHKAMSMAHANAPTAMLLAARHPPGVGERILAGFAKTFNKPQGRCVDCHTEHEGAGPM
                     AATPQKFCADCHDGMSARLKTAGHPTTLADAADFGTSHPEFRPLVRPAAGGKPVRTTL
                     AKGIVDYNGLKFPHDMHLQATGGVARMAASFRGRHDFGKKLECENCHRVEADGVRVKP
                     VEMERDCAMCHSLAFETVGGVTRTLRHGDPDQVAADLTAYYRSAPPARPLQLGGMQRR
                     RPGAYAEGQVYNIYFNEVAVRPSRAQDAVRAVFSKGGACYDCHTIFEPRAGNNWRVAA
                     VDQTPRYLQKGWFDHDAHKETACADCHVAAPASKASTDLLVPGVRQCRDCHVGENGAR
                     LVKVETATESPCAMCHEYHSDGGKPWMPQRQRRKNVTAITNKPLRATYGVSLITGTGG
                     RGLYEVTWRTPALHGARRGG"
     gene            2218..3069
                     /locus_tag="VN034_01865"
     CDS             2218..3069
                     /locus_tag="VN034_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWV59371.1"
                     /translation="MLIAQITDIHIGFDPDNPAEYNRERLDRVLGMLIDGPNRPDLLL
                     ATGDITDRGDGESYRRLAEIFARCPFPVWPSVGNHDLRDNFHAHFPRFDDGNGFVQYT
                     VELPELRLVTVDTLEEGRHGGAFCAQRAAWLDAELAKDTAKPTYIVMHHPPIESGIEW
                     MNTDPDEPWVGIFTDVVRRHSQVRGLICGHLHRSVTVAWEGRTIAICSSTAPQVSLDL
                     RPIDANRPDDRPMIVAEEPAYALHRWNGRELVSFYDNAGARPTLAKYDERLQPLVREL
                     KAERPGQ"
     gene            complement(3063..4007)
                     /locus_tag="VN034_01870"
     CDS             complement(3063..4007)
                     /locus_tag="VN034_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HWV59372.1"
                     /translation="MSKTVTVLSGLIRPLVEDRLPDWVEPRFFASKEEALELAPQAEI
                     GWFDMYDKKDMAAVITAATQLKWLNSIYAGVDGMPLDLLAERGTVVTNGAGINAITIA
                     EYVVMGMLTVAKGYRDVVRAQERREWLIDSPGKVELFGSKALLLGYGAIGKLIEERLK
                     AFAVDVTVVRRSPGPNTLTPDQWRAKLGDFDWVILAVPATPETDGMIGAAELAAMKPS
                     ATLINIARGSVVDQDALVAALDSQQIASAFLDVTTPEPLPEGDPLWSLDNAHITMHLS
                     GRAQDKMFVRSAQRFLENLDRWKKGEAVEPRVDLTLGY"
     gene            complement(4123..5565)
                     /gene="cysS"
                     /locus_tag="VN034_01875"
     CDS             complement(4123..5565)
                     /gene="cysS"
                     /locus_tag="VN034_01875"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWV59373.1"
                     /translation="MTDAPALPLKLFNSLTRSLEIFEPVHPGEARVYSCGPTVYNYPH
                     IGNMRAYVFADTLGRTLSYKGYKLTHVINITDVGHLTDDADAGEDKLEKAAAEKAQSI
                     WDIARHYTEAYWADVKALNIREPAHWSIATDYVPQMIEFAKAIADRHCYELDGGLYFD
                     TTTVSDYGRLARHGTDEGESRIDPVDGKRHPADFAIWRRTPPGEKRQMEWDSPWGRGA
                     PGWHLECSVMSEALLGFPFDIHTGGIDHREIHHPNEIAQNQAHSCSDASGARLWMHNN
                     FLVERSGKMSKSSGEFLRVQLLIDKGYHPLAYRLMCLQAHYRSELEFSWEGLGAALTR
                     LKRLVMAAAPVREAEAIEATDRRLADLLARFDAAMSDDLNTPVALTLLEEVAAMKKID
                     AGQKRATLAAMDAVLGLDLLTIDRAALRVRPRAATIGEDEIDAILARRKDARAAKDFA
                     ASDALRDELGAAGVEVMDGDPLGWEWRLAT"
     gene            complement(5615..>7253)
                     /locus_tag="VN034_01880"
     CDS             complement(5615..>7253)
                     /locus_tag="VN034_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59374.1"
                     /translation="GGGVRALGDAALTAGTAIGLTSVEGQNVTLTGTTGIDVEAANIL
                     GAANLNSGDGDIRIGSLLAAGPIDASADAIRIEGGGNLAFATLNTDVGDAYIRANGDL
                     SVADGNVAGTADFGTQGEGMSIGTLNAANAILANSGGFLNLDSVTVAGSLDASALASL
                     GITGVVTGQSIALASANITIGSAGRVGTAGVTQQLSVANNNSDNQTFVGGASGTSVAG
                     YHIDADELTRLYASQIEILAPAVQAVGSSSVGSAAPPDVIVDTFTMAGGAAGSNLGAN
                     GALTIRTPGKMRVIGNVELTGLTDANTLNLVAGDALEVILGQGSVRLVNGADPAGQLN
                     LVSDDVIVATAEAIADVGAATSTSAINTRLAQNDGILLDEGALFARGIRAEVVGGFYV
                     QNSGAGTDLAQRRGLTFGAGGLDVVTEGPSRIVVNGVGLGPNGQVTGLDALQLLTIRG
                     AAPVVGSYDRGSTVNGCLISSPTVCATVVIDIGNNFPVQDVIEEIDGDEESEGEGNTL
                     PQPLITMREVDPLTGAPLLDDPVTGAGNDDLWTPPAQ"
BASE COUNT         1154 a         2378 c         2483 g         1238 t
ORIGIN      
        1 gaccgctgcc gcctatcgcg cgctgtcggc atcgcgcagc agcgatgccg gcgcgcgcca
       61 aaaggtggtc gggctgctgt cgcagaagca agccgcgctc gcccggctgc gcaagcatct
      121 tcgcatccgg gcgctgctcg aaatctggct ttatgtccat gtgccgttga ccttcgcgct
      181 gatcgcagcg ctgtcggcgc acatcatcag cgtcttcttc tattggtgag gcgcggacga
      241 tgagtttcat tctccgccgc atcgcgacga cgaagacggg caagcaaatc gtccgcgacc
      301 aggcgctgcc cggcgacgcg atcacgctcg ggcgcgagag cggcaatatc atccatatcg
      361 ccgacctcgc ggtgaacccc caccatgcga cgatcagcag cgccgacggg cgtcatgtgc
      421 gcgtcgcggc gctcgagggg ctgggcttcg atttcaacgg ccgcggcgtc gatgtggccg
      481 acatcgacag cgccgccggc gccgagttgc gctttggcgg gcaccggctg acgatcgcgc
      541 gcgaggatga aaagatcatc ctgctcgtcg agcggatcga cgagctgtcg cagtcatcga
      601 aggacgtcga tgaggctaag gccttttcgc tcgcgggggt ggtgcccggc aagcgcatgg
      661 gggcttgggt tttcgggctg ctgatgctgc tcgccttcct gatcgggccg atctgggcgt
      721 ggtacagcta taaaaatgtg aacgagcgtc ccgacggcta tcatgccgac agcgcctggc
      781 tgtcgggccc gctgtcgagc gcgcatgcga gcctgaagga cgactgccag tcgtgccacg
      841 tcgagccctt cgtcgcggtg accgacaagg cgtgcgttaa ttgccacacg ggcgagcaca
      901 aggcgatgag catggcgcat gcgaacgccc cgaccgcgat gctgctcgcc gcgcgccacc
      961 cgcccggggt gggcgagcgt atcctcgctg gctttgccaa gaccttcaac aagccgcagg
     1021 gacgctgcgt cgattgccat accgagcatg agggcgcggg gccgatggcg gcgacgccgc
     1081 agaaattctg cgccgactgc cacgacggca tgtcggcgcg gctcaagacc gccgggcatc
     1141 cgacgacgct cgccgacgcc gccgatttcg gcacgagcca tcccgaattc cgcccgctcg
     1201 ttcgccccgc ggccggcggc aagccggtgc gcacgacgct ggcgaagggg atcgtcgatt
     1261 acaacggcct caaattcccg cacgacatgc accttcaggc gaccggcggt gtggcgcgca
     1321 tggcggcgag tttccgcggc cgtcatgatt tcggcaagaa gctcgaatgc gaaaattgcc
     1381 accgcgtcga ggccgacggg gtgcgcgtca agccggtcga gatggagcgc gactgcgcga
     1441 tgtgccacag cctcgctttc gaaaccgtcg gcggggtgac gcggacgctt cgccatggcg
     1501 atcccgatca ggtcgccgcc gacctcaccg cctattaccg gtcggcgccg ccggcgcggc
     1561 cgctccaact cggcggtatg cagcggcggc ggccgggcgc ctatgccgag gggcaggtct
     1621 acaacatcta tttcaacgaa gtcgcggtgc ggccgagccg ggcgcaggat gcggtgcgcg
     1681 cggtcttttc gaagggcggg gcctgttacg actgccacac gatcttcgaa ccgcgggcgg
     1741 gcaacaactg gcgcgtcgcg gcggtcgatc agacgccgcg ctatctccaa aagggctggt
     1801 tcgaccatga cgcgcacaag gaaaccgcgt gcgccgattg ccacgtcgcg gcgcccgcgt
     1861 cgaaggcgtc gaccgacctg ctcgtgcccg gcgtccgcca gtgccgcgac tgccatgtgg
     1921 gcgaaaatgg cgcgcggctg gtaaaggtcg aaaccgcgac cgaatcgccg tgcgcgatgt
     1981 gccacgaata tcattcggac ggcggcaagc cgtggatgcc gcagcgccag cgcaggaaaa
     2041 atgtgaccgc aatcacaaat aaacctctgc gtgctaccta tggtgtgagc ctgatcacag
     2101 gaaccggggg gcggggcctt tacgaagtga catggcgcac acccgccttg catggggcaa
     2161 gacggggcgg ataggttttg ggcggtcggg gggaaccgac cgggacgggg ggccgatatg
     2221 ttgatcgcac agatcacgga tattcatatc gggttcgatc cggacaatcc ggccgaatat
     2281 aatcgcgagc ggctcgaccg ggtgctgggc atgctgatcg acgggccgaa ccgccccgac
     2341 cttttgctcg cgaccggcga catcaccgac cgcggcgacg gggagagcta tcgccggctc
     2401 gcggagattt tcgcgcgctg cccctttccg gtctggccga gcgtcggcaa ccacgatctg
     2461 cgcgacaatt tccacgcgca cttcccccgt ttcgacgacg gcaacggctt cgtccaatat
     2521 acggtcgagc ttcccgaatt gaggctggtg acggtcgata cgctcgagga aggtcgtcat
     2581 ggcggggctt tctgcgcgca gcgcgcggcg tggctcgacg ccgaactggc gaaggatacc
     2641 gccaagccga cctatatcgt catgcaccat ccgccgatcg aaagcgggat cgagtggatg
     2701 aacaccgatc cggacgagcc gtgggtgggg atctttaccg acgtcgtgcg ccggcacagc
     2761 caggtgcgcg ggctgatctg cggccatctc catcgcagcg tgacggtcgc gtgggaaggg
     2821 cgcacgatcg cgatctgctc atcgaccgcg ccgcaggtgt cgctcgacct tcgtccgatc
     2881 gacgcgaacc gtcccgacga ccgcccgatg atcgtcgccg aagagcccgc ctatgcgctt
     2941 caccgctgga acggacgcga actcgtcagc ttctatgaca atgcgggcgc gcgcccgacg
     3001 ctcgccaaat atgacgagcg gctccaaccg ctcgtgcgcg aactgaaggc cgagcggccg
     3061 ggtcagtaac ccagcgtcag gtcgacgcgc ggctcgacgg cttcgccctt tttccagcga
     3121 tcgaggtttt cgaggaagcg ctgcgccgag cggacgaaca tcttgtcctg cgcgcgtccc
     3181 gacaggtgca tcgtgatgtg cgcgttgtcg aggctccaca gcgggtcgcc ctctggcagc
     3241 ggttcgggtg tggtgacgtc gaggaaagcc gacgcgatct gctggctatc gagcgcggcg
     3301 acgagcgcgt cctgatcgac gacgctgccg cgcgcgatgt tgatcagcgt cgccgacggc
     3361 ttcatcgccg cgagttcggc ggcgccgatc atgccgtcgg tttcgggggt cgcggggacc
     3421 gcgaggatca cccagtcgaa atcgccgagc ttcgcgcgcc actggtcggg ggtgagcgtg
     3481 ttcgggccgg gcgagcggcg caccacggta acgtcgaccg cgaacgcctt cagccgttcc
     3541 tcgatcagct tgccgatcgc gccatagccg agcagcagcg ccttcgaccc gaacagctcg
     3601 accttgcccg gcgagtcgat cagccattcg cggcgttcct gcgcgcggac gacatcgcga
     3661 tagcccttgg cgacggtgag catgcccatc acgacatatt cggcgatggt gatcgcgttg
     3721 atcccggcgc cattggtgac gaccgtgccg cgctcggcga gcaggtcgag cggcatgccg
     3781 tcgacgccgg cgtagatcga attcagccat ttgagctgcg tcgcggcggt gatcaccgcg
     3841 gccatgtcct tcttgtcgta catgtcgaac cagccgattt cggcctgagg cgcgagctcg
     3901 agcgcctctt ccttcgaggc gaagaagcgc ggttcgaccc aatcgggcag gcggtcttcg
     3961 acgagcggac ggatgaggcc ggacaggacg gtgacggttt tggacatcta ttctcccctc
     4021 ccgcttgcgg gaggggcccg ggggagggcc tgtccgcttg cggcgatcga tttgcgggag
     4081 aaacatgccc tcccctaacc cctcccgcaa gcgggagggg aactatgttg ccagccgcca
     4141 ctcccagccg agcggatcgc cgtccatcac ctcgaccccc gcagcgccga gttcgtcacg
     4201 gagcgcgtcg gaggcggcga agtccttggc ggcgcgcgcg tccttgcggc gcgcgaggat
     4261 cgcatcgatt tcgtcctcgc cgatcgtcgc cgccctgggg cggacgcgca gagcggcgcg
     4321 gtcgatcgtc agcaggtcga ggccgagcac cgcgtccatc gccgcgagtg tcgcgcgctt
     4381 ctggcccgcg tcgatctttt tcatcgccgc cacctcttcg agcaaggtca gcgcgaccgg
     4441 cgtgttgagg tcgtccgaca tcgccgcgtc gaacctggcg agcagatcgg cgaggcggcg
     4501 gtcggtcgct tcgatcgctt cggcctcgcg gaccggcgcc gccgccatca ccagccgctt
     4561 caatcgcgtc agcgcggcgc caaggccttc ccaactgaat tcgagctcgc tgcgataatg
     4621 cgcctgcaag cacatcaggc gataggcgag ggggtgatag cccttgtcga tgaggagctg
     4681 gacgcgcagg aactcgccgc tcgacttcga catcttgccc gagcgttcga cgaggaagtt
     4741 attgtgcatc cacagcctcg cgccgctcgc gtcgctgcaa ctgtgcgcct gattctgcgc
     4801 gatctcgttc gggtggtgga tctcgcggtg gtcgatgccg ccggtgtgga tgtcgaacgg
     4861 aaagccgagc aaggcctccg acatcaccga gcattcgaga tgccagcccg gcgcgccgcg
     4921 gccccacggc gaatcccatt ccatctggcg tttctcgccc ggcggcgtcc tgcgccagat
     4981 cgcgaagtcg gcggggtgac gcttgccgtc gaccggatcg atgcggcttt cgccctcatc
     5041 ggttccgtgg cgcgcgaggc ggccataatc gctgaccgtc gtcgtgtcga aatagaggcc
     5101 gccgtcgagc tcgtagcaat gcctgtccgc gatcgccttg gcgaattcga tcatctgcgg
     5161 gacatagtcg gtcgcgatcg accagtgcgc gggctcgcgg atattgagcg ccttcacgtc
     5221 ggcccaatag gcttcggtat aatggcgcgc gatgtcccag atcgactgcg ctttttcggc
     5281 ggcggccttt tccagcttgt cctcgcccgc atcggcgtcg tcggtcagat ggccgacgtc
     5341 ggtgatgttg atgacgtggg tgagcttgta gcccttgtac gacagcgtgc ggccgagcgt
     5401 gtcggcgaag acataagcgc gcatattgcc gatatgcggg taattataga ccgtcgggcc
     5461 gcagctgtag acgcgcgctt cgccggggtg gacgggctcg aagatttcga ggctgcgcgt
     5521 caggctgttg aacagcttga gcggaagggc gggggcgtcg gtcatgaccg cgcccatgcc
     5581 ttgctccgcc ggtcggcgtc aaccgaaacg ctcgctactg cgcaggcggc gtccacagat
     5641 cgtcgttgcc cgcgccggtc accggatcgt cgagaagcgg cgcgccggtc agcgggtcga
     5701 cctcgcgcat cgtgatcagc ggctgcggca gcgtgttgcc ttcgccctcg ctctcttcgt
     5761 cgccgtcgat ttcctcgatg acatcctgca ccgggaaatt gtttccgatg tcgatgacga
     5821 cggtggcgca gacggtgggg ctggagatga ggcagccgtt gacggtcgac ccccgatcat
     5881 agctgccgac gaccggcgcc gcgccgcgga tggtcagaag ctggagcgcg tcgaggccgg
     5941 tcacctggcc gttcgggccg agtccgacgc cgttgacgac gatgcgcgag ggcccttcgg
     6001 tgacgacgtc gagcccgccg gcgccgaagg tcagcccgcg gcgctgggcg aggtcggtgc
     6061 ccgccccgct gttctggaca tagaagccgc cgacgacctc ggcgcggatg ccgcgcgcga
     6121 agagcgcgcc ttcgtcgagc aggatgccgt cattctgtgc gagccgcgtg ttgatcgcgc
     6181 tggtcgaggt cgccgcgccg acatccgcga tcgcttcggc cgtggcgacg atgacgtcgt
     6241 ccgagaccag gttgagctgt cccgccggat cggcgccgtt caccagccgc acgctgccct
     6301 gcccgaggat cacctcgagc gcgtcgccgg cgacgagatt gagcgtattg gcgtcggtga
     6361 gtccggtcag ttcgacattg ccgatcacgc gcatcttgcc cggggtgcgg atcgtcagcg
     6421 cgccgttggc gccgaggttc gatcccgccg cgccgcccgc catggtgaaa gtgtcgacga
     6481 tgacgtcggg cggcgcggcc gacccgaccg aactgctgcc cacggcctgc accgcgggcg
     6541 cgaggatctc gatctggctg gcatagaggc gggtcagttc gtcggcatcg atatgatagc
     6601 ccgcgacgct ggtgcccgac gcgccgccga cgaaggtctg gttgtcgctg ttgttgttcg
     6661 cgaccgacaa ttgctgcgtg acgcccgcgg tgccgacgcg gcccgccgag ccgatcgtga
     6721 tattcgccga ggcaagcgcg atcgactggc cggtaacgac gccggtgatc ccgagcgacg
     6781 cgagcgcgct ggcgtcgagg ctacccgcga cggtcacgct gtcgaggttc aggaacccgc
     6841 ccgaattggc gaggatcgca ttggccgcgt tgagcgtgcc gatgctcatg ccttcgccct
     6901 gcgtgccgaa atcggccgtg cccgcgacat tgccgtccgc gaccgacagg tcaccgttcg
     6961 cccggatata ggcgtcgccg acgtcggtat tgagcgtcgc aaaggcgagg ttgccgccgc
     7021 cttcgatgcg gatcgcatcg gcgctggcat cgatcgggcc cgcggcgagc aggctgccga
     7081 tacggatatc gccgtcgccg ctgttcaggt tggccgcgcc gagaatattg gccgcctcca
     7141 cgtcgatgcc ggtcgtgccg gtcagcgtga cattctggcc ttcgaccgag gtgaggccga
     7201 tggcggtgcc cgccgtcagc gccgcgtcgc cgagcgcgcg aacgccgccg ccc
//
LOCUS       DATMSU010000072         2794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_162427,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000072 DATMSU010000000
VERSION     DATMSU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162427"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VN034_01885"
     CDS             complement(<1..1189)
                     /locus_tag="VN034_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV59375.1"
                     /translation="MRPATGEDGDHVTFCRICTAVCGLVATVEDGKVVRARPDPDNPS
                     SQGHACVKGIAYHGVTHDPDRVTRPMKRVAPGRFEPVGWDEALDDIANRLSAIIAEHG
                     PDAVASYQGNPPAYATDGMTGFRMFLKALGTTKIYGAGSQDTNARFTANWILYGSPLT
                     IDVPDVNNADFMLIFGANPLISHGSLIFTPRVRHQLDAVAARGGVVVVDPRRSETAAR
                     YEHVAIEPNADCWLMLAMLKTLADEGLADEAFLAERCAGWPELRAALAGIDYDDAARR
                     TGIDVETIKSLARRLTASPKSVCYGRVGICRGPHATLANFLLSALNMVGGTFGHEGGS
                     TFATPVLAGSDRATTGGYDEVRSRVGNFPSVTQFLPSAVMPDDILEPGPGKVRALLSL
                     GGNV"
     gene            1415..2611
                     /locus_tag="VN034_01890"
     CDS             1415..2611
                     /locus_tag="VN034_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV59376.1"
                     /translation="MADVKDYAVEEFSQHDRALMQRPYDFYDKVRAGGCPVARSEQLG
                     GFWYTTNYAATRRVYDDFRTFSSADGTALPKQPLPLYPIDLDPPQQTRMRKLLNPIFL
                     PDAIQKYRPRFEAIITELLDDIAPKGEAELQEQLVRPTLATVIMPFLGVPRDDWEMLA
                     HKLDFLTRMRVEDPETCAKYGVELSDYLYEFATRRRGTPPEDDLMQILIDARINDEAL
                     TDEEIVGIATLVLFGGLDTTSAVVGTALWYLIEHPGDRARLLGGEVDFPRALHEFVRY
                     TSPIQGLRRTVTRDTELEGCPLKAGDYVMAMNGAANHDPGKFPEPDQVRFDRPIGNEH
                     DHLGFGGGAHICIGQHFAKTLMETLIRAIFDRLPDLRIQPDFQPAFAVGESRVLKTLP
                     VTFRAS"
BASE COUNT          521 a          906 c          881 g          486 t
ORIGIN      
        1 gcacattgcc gccgagcgac agtagcgcgc ggaccttgcc ggggccgggt tcgaggatgt
       61 cgtcgggcat caccgccgag ggcaggaatt gcgtcacgct cgggaaattg ccgacacggc
      121 tgcgtacctc gtcatagccg ccggtcgtgg cgcggtcgct gcccgcgagc accggcgtcg
      181 cgaaggtgct gccgccctcg tgaccgaaag tgccgccgac catgttgagc gccgagagca
      241 ggaaattggc gagcgtcgca tgcgggccgc ggcagatgcc gacgcggccg tagcagaccg
      301 atttcgggct ggcagtgagc cggcgcgcga gggacttgat cgtctcgacg tcgatgcctg
      361 tgcgccgcgc ggcatcatcg taatcgatgc ccgcgagcgc agcgcgcaat tcgggccagc
      421 cggcgcaacg ttcggccaga aacgcctcgt cggccagccc ctcgtcggcg agcgtcttga
      481 gcatcgcgag catcagccag caatcggcat tgggctcgat cgcgacatgt tcgtagcgcg
      541 cggcggtctc gctccgccgt gggtcgacaa caacgacccc gccgcgcgcc gcgaccgcgt
      601 cgagctggtg ccggacgcgc ggagtgaaga ttagcgatcc gtgcgagatc agcggattgg
      661 ccccgaaaat cagcatgaag tcggcgttgt tgacgtcggg cacatcgatc gtcagcgggc
      721 tgccgtagag gatccagttg gcggtgaagc gggcattggt gtcctgcgag cccgcgccat
      781 agattttcgt cgtgcccagt gccttcagga acatgcggaa ccccgtcatg ccgtcagtcg
      841 cgtaggcggg cggattgccc tgatagctgg cgaccgcatc cggcccatgc tcggcgatga
      901 tcgccgacag ccggttcgcg atatcgtcca gcgcctcgtc ccagcccacc ggttcgaacc
      961 gccccggcgc gacgcgcttc atcggccgcg tcacccggtc gggatcgtgc gtcacgccat
     1021 gataggcgat gcccttgacg caggcatggc cctgcgacga cggattgtcg gggtccggcc
     1081 gcgcccgtac caccttgccg tcctcgaccg tcgccaccag cccgcacacc gcggtgcaga
     1141 tgcggcagaa agtcacatgg tcgccgtcct cgcccgttgc tggccgcatg attcgctccg
     1201 gttaattgat cgattagaac ctttcattct tttcgccagc tgtccagatt tctgggaatg
     1261 taaagtcagc caacggcatg gaaatcacca taaggcgccg attcctctca cattgagccg
     1321 aacagcaatt aatcgattgg ttgacacgac tggccatttg atcgaaattg cctcgcgaaa
     1381 agacccatgg gcataaaggc gagaggatcg agcgatggcg gacgtcaaag attatgcggt
     1441 ggaagagttc agccagcatg accgcgcgct gatgcagcgc ccctacgatt tttacgacaa
     1501 ggtgcgcgcg ggcggctgcc cggtcgcgcg gtcggagcag ctcggaggct tctggtacac
     1561 gaccaactat gccgcgacgc ggcgcgtcta tgacgatttc cgcaccttca gctcggccga
     1621 cggcaccgcg ctgccgaagc agccgctacc gctctatccg atcgacctcg acccgccgca
     1681 gcagacgcgg atgcgcaagc tgctgaatcc catctttctg cccgacgcga tccagaaata
     1741 tcgcccgcgc ttcgaggcga tcatcaccga acttctcgac gacatcgcgc cgaagggtga
     1801 ggccgaactg caggagcagc ttgtccggcc cacgctcgcg acggtcatca tgcctttcct
     1861 tggcgtgccg cgggatgatt gggagatgct ggcgcacaag ctggacttcc tgacccggat
     1921 gcgggtcgag gatcccgaaa cctgcgcgaa atatggcgtc gagctcagcg actatctcta
     1981 tgaatttgcg acgcggcggc gcggcacgcc gcccgaagac gatctgatgc agatattgat
     2041 cgacgcgcgc atcaacgacg aggcgctgac cgacgaggaa atcgtcggca tcgcgacgct
     2101 cgtcctgttc ggcggcctcg acacgacgag tgccgtcgtc ggtacggcgc tctggtatct
     2161 gatcgaacat cccggcgacc gcgcccgcct gctggggggc gaggtggatt ttccccgcgc
     2221 actgcacgaa ttcgtccgtt acacctcgcc gatccagggg ttgcggcgaa ccgtcacgcg
     2281 cgacaccgaa ctcgaaggat gcccgctgaa ggcgggggac tatgtcatgg cgatgaacgg
     2341 ggcggccaat cacgatcccg gcaaattccc ggaacccgac caggtgcgct tcgaccggcc
     2401 gatcggaaac gaacatgacc atctcggctt cggcggcggg gcccatatct gcatcgggca
     2461 gcatttcgcc aagacgctga tggaaacact gatccgtgca atattcgacc gcctgccgga
     2521 ccttcggatc cagccggatt ttcagcccgc tttcgcggtc ggcgagtcgc gggtgctgaa
     2581 gacattgccg gtcaccttcc gcgcatcctg accatcctat aggtggaccg caacccgcgt
     2641 tccgccccat cgacaggatc atgatgtgct gaagctgacc gctgaagaaa ccacctttca
     2701 aggcgaagtg cgcgcctttt tcgcgcgcga ctatcccgca catgtcatcg cgaagaacca
     2761 ggcggggcag cggctgacga aacaggacca tatc
//
LOCUS       DATMSU010000073         5775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_105793,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000073 DATMSU010000000
VERSION     DATMSU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105793"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="VN034_01895"
     CDS             <1..96
                     /locus_tag="VN034_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HWV59377.1"
                     /translation="LNRLSDHLFVLTRHLNAAAGGDILWKPGATR"
     gene            267..1532
                     /gene="egtB"
                     /locus_tag="VN034_01900"
     CDS             267..1532
                     /gene="egtB"
                     /locus_tag="VN034_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HWV59378.1"
                     /translation="MASRTDASPRTDPIEALNRRFAATRRLSLDLAGDLSDADASAQS
                     MPDASPAKWHLAHTTWFFETFVLRDHVPGYALFDARYPYLFNSYYEAEGPRHARPQRG
                     LLTRPSLDEICVWRAHVDAAVQSALPDLPASALALVTLGIHHEQQHQELLLTDIKHLF
                     AQNPLGPAVWDTVIASEVRQFSAMKWVKGAEGIVSIGHGGDGFAFDCEGPRHEVLLTP
                     HALASRPVSNGEWQEFIADGGYHTPALWLSDGWAWVQAEDVAAPAYWRDGQLFTLSGW
                     QDIDPAAPVTHISFFEADAFASWAGARLPTEQEWEAAAAGLDPDGGQQLDAAGPVQPA
                     PEDGDTDLQQMFGSVWEWTGSAYRPYPGFRAAPGAVGEYNGKFMSGQFVLRGGSCATP
                     RGHCRATYRNFFYPHQRWQFTGLRLAKDL"
     gene            1535..2521
                     /gene="egtD"
                     /locus_tag="VN034_01905"
     CDS             1535..2521
                     /gene="egtD"
                     /locus_tag="VN034_01905"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWV59379.1"
                     /translation="MGVVRNLRQVSADDAGIDIAFRADVHAGLAQRQKAIPARWFYDA
                     TGSALFEDITALPEYYPTRSETDLLTRHAADIASAIGPARAVVELGSGSSTKTPLLLD
                     AIAPAAYVPVDISGDFLRDSAEALAARFPAVPIYPVEADFTQRVELPREICPLPKLGF
                     FPGSTIGNMVARTAIDLLRNWRATLGDGSLMLIGIDRIKDIGILETAYDDPAGVTAAF
                     NLNLLERINRELGGDIAVENFSHRAVWDDVHARIEMHLVAACDMDFTIDGRAYHMAKG
                     ETIHSENSHKYGARDANLLLRAGGWTPVATWDDADPAFALILAEATEFRSAP"
     gene            2590..3456
                     /locus_tag="VN034_01910"
     CDS             2590..3456
                     /locus_tag="VN034_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWV59380.1"
                     /translation="MIVGVIGAGQMGAGIAQVSAGAGHDVLLSDIDIARAEAGKAGIA
                     KALGRLVAKEKMSQGDADALLGRITPVADHSAFAPADLVVEAATEREEIKRAIFASVG
                     EHLSATAILASNTSSIPITRLAQAAPDPARFIGVHFFNPVPVMGLIELIRGLATSDDT
                     LAKVEAYGRGLDKEIVHANDAPGFIVNRVLMPLINEAVFALGEGVATMQDIDTGCRLG
                     LNHPMGPITLADFIGLDTCLEIIRVLYSGTGDPKFRPAPLLVQYVDAGWVGKKAGRGF
                     YDWSGGEPVPTR"
     gene            3453..4055
                     /locus_tag="VN034_01915"
     CDS             3453..4055
                     /locus_tag="VN034_01915"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006831953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV59381.1"
                     /translation="MSGSDAPTLAFYAAEAPVYSASGPSGVSRHLDRFLDRLSAGAEI
                     LELGCGGGRDAAHMIARGFAVDPTDGVAEIAAQAEARVGRPVRVMRFGELAAVDSYDA
                     IWASASLLHVPRVDLPGVLARIHRALKPGGLHFASYKSGGREGRDRFGRYFNYFAREE
                     LEAVYREAARWDWLDLAEGLGGGYDGVQGPWVQITVRRPL"
     gene            4123..4611
                     /locus_tag="VN034_01920"
     CDS             4123..4611
                     /locus_tag="VN034_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59382.1"
                     /translation="MRNKALFALAAILLAGTASAHAQQGAPEESTIKDTANSVTEPFD
                     GKEVPPKLLEVQGDPYSLAGLGKCAAIIAEVNELNAVLGPDVNEQVDKDRAKKREETA
                     GRVAGTVAGGIIPFRGLIGEVTGANAERRRYALAVYAGTVRRGFLKGVGLQRGCKAPA
                     RP"
     gene            complement(4621..4938)
                     /locus_tag="VN034_01925"
     CDS             complement(4621..4938)
                     /locus_tag="VN034_01925"
                     /inference="COORDINATES: protein motif:HMM:NF021390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HWV59383.1"
                     /translation="MKTMTVFAAAAALLALAACSSVPRNTGTSYDCNRGTQLKVDYVG
                     NTAIVRVNGRRSMVLKQTPSTGGAVYENKTGARLQRNGNDVTWNTALRSAPESCRVVY
                     TPL"
     gene            complement(4952..5572)
                     /locus_tag="VN034_01930"
     CDS             complement(4952..5572)
                     /locus_tag="VN034_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HWV59384.1"
                     /translation="MKTAYRLSILAAAAASIGAGFLATSDAQTVETERYRPPEATIYR
                     DAGYRGPAVFIGEEKPNLRLAWPVNSIRVTRGRWELCERTRYRGNCRTVDRDTPMLNN
                     VLRGITVQSIRPAGSGGGGGGWNPNPPANDQVARGNFAEFHTQPAERNYRVLACARGS
                     ATANCAAQTADNWCRSIGWNGSAREHMETVSGRVYLADVLCVRSGY"
BASE COUNT          965 a         1968 c         1867 g          975 t
ORIGIN      
        1 ctcaaccgcc tttcggacca tcttttcgtg ctcacgcggc acctcaacgc cgcggcaggc
       61 ggcgatatcc tctggaaacc cggcgcgacg cgctaaaacc gtcgcccccg tgcgcacggg
      121 ggccgctgga gaggtgtgca aggccgacgg ccgtcgcatg cgcggccacg attaatgttc
      181 cccaaatgtt ctagacaatc gatcgcgctg taaccataat ccgacgaacc gcgaaaccga
      241 atcgcacccc gggggattgt ctcgacatgg ccagccgcac cgacgcttcg ccccgcaccg
      301 atccgatcga ggcgctgaac cggcgcttcg ccgcgacgcg ccgtctttcg ctcgatctcg
      361 cgggcgatct ttccgacgcc gacgcgagcg cgcaatcgat gcccgacgcc tcgcccgcca
      421 aatggcacct agcgcacacg acctggttct tcgaaacctt cgtcctgcgt gaccatgtgc
      481 cgggctatgc gctcttcgac gcgcgctatc cctatctttt caactcctat tatgaggccg
      541 agggcccgcg ccacgcgcgt ccgcagcgcg ggctgctgac gcgcccgtcg ctcgacgaaa
      601 tttgcgtctg gcgcgcgcat gtcgacgcgg cggtgcagag cgccttgccc gatctccccg
      661 cgagcgcgct ggcgctcgtc accctcggca tccatcacga gcagcagcat caggaattgc
      721 tgctcaccga catcaagcat ctcttcgcgc aaaatccgct tggcccggcg gtgtgggaca
      781 cggtgatcgc gagtgaagtt cggcagtttt ccgccatgaa atgggtgaag ggcgccgaag
      841 ggatcgtctc gatcggccat ggcggcgacg gcttcgcctt cgactgcgaa gggccgcgcc
      901 acgaggtgct gctgacgccg cacgcgctcg cgagccgccc cgtgagcaac ggcgaatggc
      961 aggaattcat cgccgacggc ggctatcaca cccccgcgct ctggctcagc gacggctggg
     1021 cgtgggtgca ggccgaggat gtcgcggcac ccgcctattg gcgggacggc cagcttttca
     1081 ccctgtcggg ctggcaagat atcgaccccg ccgcaccggt gacgcacatc agcttctttg
     1141 aggccgacgc cttcgccagc tgggccggcg cgcgcctgcc gaccgaacag gaatgggaag
     1201 cggcggccgc ggggctcgat ccggatggcg gccagcaact cgacgcggcc ggccctgtcc
     1261 agcctgcgcc ggaagacggc gacacggacc tgcaacagat gttcggcagc gtgtgggaat
     1321 ggaccggcag cgcctatcgc ccctatcccg gcttccgcgc cgcgccgggt gcggtcggcg
     1381 aatataatgg caagttcatg agcggccagt tcgtgctccg cggcggcagc tgcgcgaccc
     1441 cgcgcggcca ctgccgcgcc acctaccgca acttcttcta cccccaccag cgctggcaat
     1501 tcaccggtct gcgcctagcg aaagatcttt gaccatgggc gtcgtgcgca atcttcgtca
     1561 ggtttcggcc gatgatgccg gcatcgacat cgcctttcgc gccgacgttc acgccggcct
     1621 cgcgcagcgg caaaaggcga ttccggcgcg ctggttctac gatgcgaccg gctcggcgct
     1681 gttcgaggac atcaccgcgc tccccgaata ttatccgacg cgtagcgaaa ccgaccttct
     1741 gacgcgccac gcggccgaca tcgcgtccgc gatcgggccg gcccgcgcgg tggtcgaact
     1801 cggatcgggc agctcgacca agacgccgct gctcctcgat gccatcgcgc ccgccgccta
     1861 tgtcccggtc gatatttcgg gcgatttcct tcgcgacagc gcagaggcgc tcgcggcgcg
     1921 cttccccgcg gtgccgatct atccggtcga ggccgatttc acccagcggg tcgaattgcc
     1981 gcgcgaaatc tgcccgctcc cgaaactcgg cttttttccg ggctcgacga tcggcaacat
     2041 ggtcgcacgc accgcgatcg acctcctacg caactggcgc gcgacgctcg gcgacgggtc
     2101 gctgatgctg atcggcatcg accggatcaa ggacatcggc atactcgaaa ccgcttatga
     2161 cgatccggcg ggggtgaccg cggcgttcaa cctcaacctc ctcgaacgga tcaatcgcga
     2221 gctcggcggc gatatagcgg tcgagaattt cagccaccgc gcggtctggg acgatgtcca
     2281 cgcgcgtatc gaaatgcacc tcgtcgccgc gtgcgacatg gatttcacga tcgacggccg
     2341 cgcctatcat atggcgaagg gcgaaacgat tcacagcgag aacagccata aatacggggc
     2401 ccgcgacgcg aacctgctgc tccgcgccgg cggctggacg ccggtcgcga catgggacga
     2461 tgccgacccc gcctttgcgc tgatcctcgc cgaagcgacc gagttccgct ccgccccctg
     2521 acgctcgctc ttgacgcctc tgcccgaagc cgtagggcca cgggcagatt ttcagcgaaa
     2581 gggcaggata tgatcgtcgg cgttatcgga gcggggcaga tgggcgcggg gatcgcgcag
     2641 gtgtcggcgg gcgccggcca tgacgtgctc ctctccgaca tcgacatcgc ccgcgccgaa
     2701 gcgggcaagg cgggcatcgc caaggcgctc ggccgcttgg tggcgaaaga gaagatgagc
     2761 caaggcgacg ccgatgcgct gctcggccgg atcacgcccg ttgccgacca tagcgccttc
     2821 gcccccgccg acctcgtcgt cgaggcggcg accgagcgcg aggaaatcaa gcgcgcgatc
     2881 ttcgccagtg tcggcgagca tctgtcggca accgcgatcc tcgcgagcaa caccagctcg
     2941 atcccgatca cccggcttgc ccaggcggcg cccgatccgg cgcgtttcat cggcgtccat
     3001 ttcttcaacc cggtaccggt gatggggctg atcgaactga tacgcggcct cgcgacgagc
     3061 gacgacacgc tcgccaaggt cgaggcctat ggccgcgggc tcgacaagga gatcgtccac
     3121 gccaatgatg cgcccggctt catcgtcaac cgcgtgctga tgccgctcat caatgaagcg
     3181 gtgtttgcct tgggcgaagg cgtcgcgacg atgcaggaca tcgacaccgg ctgccgcctc
     3241 ggtctcaacc atccgatggg accgatcacg ctcgccgact tcatcgggct cgacacctgt
     3301 cttgagatca tccgcgtgct ttacagcggc accggcgacc cgaaattccg ccccgcgcct
     3361 ctgctcgtcc aatatgtcga cgccggctgg gtcgggaaaa aggccgggcg cggcttttac
     3421 gactggagcg gcggtgaacc cgtcccgacg cggtgagcgg ttccgacgcc ccgacgcttg
     3481 ccttctacgc ggccgaagcg ccggtctatt cggcgtcggg tccgtcgggc gtgtcgcgcc
     3541 atctcgatcg cttcctcgac cggttgtccg cgggggctga gattctcgaa cttggctgtg
     3601 gcggcggccg cgatgccgcg cacatgatcg cgcggggctt tgcagtcgac cccaccgacg
     3661 gggttgccga gatcgcggcc caggccgagg cgagggtcgg cagaccggtc cgggtgatgc
     3721 gcttcggcga actcgccgcc gtcgacagct acgacgccat ctgggcatcg gccagcctgc
     3781 tccacgtgcc gcgcgtcgat cttcccggcg tgctcgcgcg tatccatcgc gcgctgaaac
     3841 ccggcggact gcatttcgcg agctacaaga gcggcggacg cgaggggcgc gaccgtttcg
     3901 gacgctattt caactatttt gcgcgcgagg aactcgaagc cgtttaccgc gaagccgcac
     3961 gctgggattg gctcgatctc gccgaaggtc ttggcggcgg ctatgacggc gtgcaggggc
     4021 catgggttca aatcaccgta cgccgcccgc tataggacgg tggagggagc cgaaaggcgt
     4081 tgggcgcgtt gaatctgcga gaatgcaggg gatgatatga ttatgcgtaa caaggcctta
     4141 ttcgctctcg cggcaatttt gctcgccgga accgcatccg cccacgccca gcagggcgcg
     4201 cccgaagaat cgacgatcaa ggacacagcc aattcggtga ccgaaccgtt cgacggcaag
     4261 gaggtgcctc ccaagctgct cgaagtgcag ggcgatccct attcgctggc gggcctcggc
     4321 aaatgcgccg cgatcatcgc ggaggtgaac gaactcaacg ccgtgctggg ccccgacgtc
     4381 aacgagcagg tcgacaagga tcgcgcgaaa aagcgcgagg aaaccgccgg ccgggtcgcc
     4441 ggaacggtgg cggggggcat catccccttc cgcgggctga tcggcgaggt gaccggcgcc
     4501 aatgccgaac ggcggcgcta tgcgctcgcc gtctatgccg gcacggtgcg ccgcggcttc
     4561 ctgaagggcg tcgggcttca gcgcggctgc aaggcgccgg cccggccctg attccgtcgt
     4621 tcagagcggc gtgtatacga cgcgacagct ctcgggcgcc gaacgcaacg cggtgttcca
     4681 cgtcacgtca ttgccattgc gctgcagccg cgcgcccgtc ttgttctcat aaaccgcgcc
     4741 gccggtcgac ggggtctgct tcagcaccat cgagcgccgg ccgttgacgc gcacgatcgc
     4801 cgtgttgccg acatagtcga ccttcagctg cgtgccgcgg ttgcaatcat agctggtgcc
     4861 ggtgttccgc ggcaccgacg agcaggccgc aagcgcaagc agcgcggcgg cggccgcgaa
     4921 aactgtcatc gtcttcattg ccggtctccc ctcaatagcc cgagcggacg cacagcacgt
     4981 cggcgagata gacccggccg ctcaccgttt ccatatgctc gcgcgccgac ccattccagc
     5041 cgatcgagcg gcaccagttg tccgccgtct gcgccgcaca attcgcggtc gccgatccgc
     5101 gggcgcaggc gagcacgcga tagttgcgct cggccggctg ggtgtggaat tcggcgaaat
     5161 tgccccgcgc gacctgatca ttggcgggcg ggttggggtt ccatccgccg ccgccgccgc
     5221 ccgaaccggc gggacgtatc gactgaaccg tgatgccgcg caggacattg ttcagcattg
     5281 gcgtgtcgcg atcaaccgtc cggcagttgc cgcgatagcg cgtcctttcg caaagctccc
     5341 agcgtccgcg agtgacgcgg atcgaattga cgggccaggc gagccgaaga ttcggctttt
     5401 cctcgccgat gaataccgcg gggccgcgat agccggcgtc gcgatagatc gtcgcctcgg
     5461 gcggacggta tcgttcggtt tcaacggtct gggcgtccga cgtggcgagg aaacccgccc
     5521 ctatcgacgc cgctgcggcg gcaaggatcg acaaacgata agcggttttc attacagcct
     5581 ccctgttgac gaacgcgacc cgggattcgt tatgttcccg caatttacgt cattgcaaag
     5641 tggcttgcaa attctcccac ttgccggtcc gcgcatgcga tacgggatcg cgggtcaggg
     5701 tgtcttcgcc ggcggtgctg atatggccgc atcgggcgtt accacgggag ccgcggcggg
     5761 ttcgggcgcc ggggt
//
LOCUS       DATMSU010000074        10214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172077,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000074 DATMSU010000000
VERSION     DATMSU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172077"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VN034_01935"
     CDS             <1..835
                     /locus_tag="VN034_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV59385.1"
                     /translation="TRDDAREPVRSDQVPLLMSLLDQASIALDRMELEEASLRARQID
                     ERDRLRSALLSSVSHDLRTPLTTILSAAQEMRRQPSPALAETVEAEARRLNRFVSNLL
                     DMVRVEAGALPMKAEATDLFDAAASAAHDTRASLAGHEVKVDIPSNIPLVRVDPVLLH
                     HCLINLLDNAGRYADPDTPIIIRAQRTSDAILLSVIDEGPGIAPGNEKRVFETFTRLE
                     GSDRAKHGTGLGLAIVKGFAEAMGLAVEAGNNEQPHGARFTIQIPGALIIAPMSDKEI
                     P"
     gene            832..1557
                     /locus_tag="VN034_01940"
     CDS             832..1557
                     /locus_tag="VN034_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV59386.1"
                     /translation="MKVRHKVLVVDDELHIRRLIHAALARNDYAIVEAETARGALEQL
                     RAERPDITLLDLGLPDRDGLELVPLFKQQSDTTLIVVSARDATDQKVAALDLGADDYL
                     TKPFDTDELLARVRVALRNRLTRDGGMLALAVEDVTIDLVARTVTKAGKEVHLTPKEY
                     GVLAQLAKFPGRVITHKQIMAEVWPREYEHHVEYLRVLVRTLRQKLETDPQQPRIICN
                     ELGIGYRLRPGNDASAEDGEPPR"
     gene            1633..1791
                     /locus_tag="VN034_01945"
     CDS             1633..1791
                     /locus_tag="VN034_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59387.1"
                     /translation="MTQDEIEAQPDTSERRIRDRMVDWLGAQGALLLTVYMCAVVAAV
                     AFLLDTAR"
     gene            1915..2256
                     /locus_tag="VN034_01950"
     CDS             1915..2256
                     /locus_tag="VN034_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59388.1"
                     /translation="MTKWHESTAANIGLRATGLTLLAAAWWLAVRLHHMSQTILPRDA
                     TTPIILLSAILFLCASAGSALLFVGPALWETVEISERWRRAPPSGSEAAEAFDGRPSR
                     TVRRPEDGASA"
     gene            2374..3192
                     /locus_tag="VN034_01955"
     CDS             2374..3192
                     /locus_tag="VN034_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HWV59389.1"
                     /translation="MKSIIIPAALLAGLSPASAFAQEEEGKAVTLSGSATVASDYRFR
                     GVSQSDKEMAVQGGLTLSHESGLYAGVWASNLAGWGSFGGANMELDLIGGYKLPISET
                     ATLDAGLVWYMYPGGADESDFAEPYVKLTGTSGPATLTAGVAYAPKQHALANVSNAPG
                     SRGQSEDNLYLWGDGAVAIAGTPFTAKAHIGYSDGNPGLGPNGTSVAPTGRYWDWSLG
                     VDTTWRNLTLGLSYVDTDIGGAEAARLQPNFSKGLDGTGSIADATIVASITAAF"
     gene            complement(3255..5156)
                     /locus_tag="VN034_01960"
     CDS             complement(3255..5156)
                     /locus_tag="VN034_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59390.1"
                     /translation="MAGIAASLAALPAHAPAFAQRADDNAIADAEDAFGSNDGGETLG
                     IYNPNSVRGFSPIDAGNVRLEGLYFDRQGDFSPRLVEGNRIRVGPSAVSYPFPAPSGV
                     VDYRLRSPGKDAMLSGVIQANSWGGALVEIDAAVPINEVLSVGGGASFSRSEYPSGNN
                     ADVTNVAAIAHWRPAPGTDLKLFGSRTRIADEDIYPIILGNGVETPGRLTRRRFLGQK
                     WADVETERYTFGAMGRTSIAGFAVRAGLFRSDNEVREGHNIFLRAAPLGTPAERIVSA
                     YPGRAGVSTSGELGVARGFSTGAFEHRIQLVARGRAQDRRYGGSIRRSLGSAPFGEAD
                     YVARPDFDFAAQSSDRVRQWSIGLDYQMRVRGLGQFSIGAQKVDYSKRVTTPSGPAPE
                     SRDTPWLINATATIDLAPAISLYASFTRGLEESDVAPETAANRDEAPPAIRTRQIDAG
                     AKLRAAGMTLIAGLFDIERPYFGVDGSNVFRRLGVVRHSGIEASLSGSPTPGLTLVAG
                     GSLIRARVKGDEVASGAIGNRPIDVPSRKIIASVDWRPPSSPTSVDAAVEHVGPNTAD
                     RLNLVEASPYTTINIGLRHRFTIGRSAAVFRVQATNLFDAYGWEVAGDNAFVYIQPRQ
                     VMARLTVDL"
     gene            5239..6144
                     /locus_tag="VN034_01965"
     CDS             5239..6144
                     /locus_tag="VN034_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWV59391.1"
                     /translation="MAHSSYDLPPLDGLTAMLAAAEEGSFSAAAGKLGLTHGSVSRRI
                     AALEHWLGASLFERHGRGVRLTPAGLRFVRDARQSLGRLSHSAEQWRPRRGRQTVRMS
                     VVPSFARLWLLRRLPAIERHDTHIDLSLDHRMTDLDSREAEVVIRYGRGTWVGVDARQ
                     IVEESLAPAASSELRGRMSDAPAIDELLQWPLLHDSDTSQWRAFLGRAGVSYRPRWQD
                     RRFEDYDTVLAAAEAGLGIALIRQPLAADWLIDGRLTTITAETMDNPASHFVCVRAGE
                     TRQAVLTLRDRILGLAAGDGSGKST"
     gene            complement(6187..6750)
                     /locus_tag="VN034_01970"
     CDS             complement(6187..6750)
                     /locus_tag="VN034_01970"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HWV59392.1"
                     /translation="MTISLFDLSVPTFLQTLRAVSGWLDRAARHGAETGTDPDDFVGA
                     RLCPDMAPFHFQIEALKNHAVWGLNAAKTGAFAPPPLVGEMPFAELQSTIVQAVAALE
                     ALTPDEVNSWSGRNLDIELFRPLDEENASTSSWAPRTFALTPETLLLTYSLPNFYFHA
                     VTAYDILRTQGVPIGKGDYEGRLRTRP"
     gene            complement(6804..7640)
                     /locus_tag="VN034_01975"
     CDS             complement(6804..7640)
                     /locus_tag="VN034_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59393.1"
                     /translation="MMKWHLKAVCMASLIVAACSPEQPGAGEDQRQSAQSEARAGATV
                     DSFHLPRDLEIQLAESALPKHLQERATIYVLNPRKGFEIARAGTNGFHAFLARTGDDA
                     FRGDWPFTAYRNDIIYPIAFDEVGSKANMRVFFDAAELQAKGTPPADLKKLIKERYAS
                     GHYKAPDHGGVSYMLSPILRTYIDPERSGEVETSTNPHIMHYAPNVSNQALGGVKPGI
                     NPLTFVIQEGPHGFAVQHLGEKEMAAIKTEYAPMLERLCGLNPLWCLPGDKEQKPGTA
                     GS"
     gene            complement(7694..8437)
                     /locus_tag="VN034_01980"
     CDS             complement(7694..8437)
                     /locus_tag="VN034_01980"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HWV59394.1"
                     /translation="MHISESESTTPKVADRATFQAELDELRLREKAHMRVGDEIAATR
                     RRLPMVAVDGAAPLIGKDGPTTLLDAFEGRRMLIAYYYMWFTGRPAERQCQGCSWFTS
                     QVRELSYIHSRDATYATFCQGPYEESVRYRDFMGWDMPWYSVTEGLDTLLAGREAGSM
                     YLICYLRRGSDVFETYWTTRRGVEAMDNSYRLLDLTVYGRQEEQEVSPPGWPQGWGEN
                     EKVHPFRSDGRPIAQWQRLAAGFSDDLGN"
     gene            complement(8578..8937)
                     /locus_tag="VN034_01985"
     CDS             complement(8578..8937)
                     /locus_tag="VN034_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59395.1"
                     /translation="MKVEKTYPSLLVADLAAAERWYTDLFGRGPDHRPMPTLLHWELF
                     DEGGLMLSTSDEIADRGSIFLYVADLAAERRRLESAGIALGDDITGDYSTLAQVRDPD
                     GNLVTLATPPSAPFPSA"
     gene            complement(9093..9563)
                     /locus_tag="VN034_01990"
     CDS             complement(9093..9563)
                     /locus_tag="VN034_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWV59396.1"
                     /translation="MNDGEAPANAREIVVDEIFLHAPETIWKTLTSGELMGRWLGMAP
                     AGFAPVVGNRFTYRTTAAGKWDGTIQCEVLEVIPNERFVYSWRGGHEENVGYGAPLDT
                     IVTFTLEPADGGTRLRLVHSGFVLPQNDSAYRNMSGGWSQVVPRIGAISDEQDI"
     gene            complement(9560..9889)
                     /locus_tag="VN034_01995"
     CDS             complement(9560..9889)
                     /locus_tag="VN034_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWV59397.1"
                     /translation="MMQTVAVDTVLRALADPTRRAVFERLARSDEVNVTALTRVSGVT
                     QGAVSQHLKALKQAGLVVERPEGRNVYYRARPEGLSPLLDWLAHYDSFWRDSFVGLRA
                     LLQEIDP"
BASE COUNT         1787 a         3308 c         3303 g         1816 t
ORIGIN      
        1 cacccgcgac gatgcccgcg agcccgtccg ctccgatcag gtgccgctgc tgatgagcct
       61 gctcgaccag gcgtcgatcg cgctcgaccg catggaactc gaggaagcat cgctgcgcgc
      121 gcggcagatc gacgagcgcg accggctgcg atcggcgctg ctctcgtcgg tcagccacga
      181 ccttcgaacg ccgctgacga cgatcctctc ggcggcgcag gaaatgcggc gccaaccctc
      241 gccggcgctc gccgaaacgg tcgaggccga ggcgcggcgg ctcaaccgct tcgtatcgaa
      301 tctgctcgac atggtgcgcg tcgaggcggg cgcgctgccg atgaaggccg aggcgaccga
      361 cctcttcgac gctgccgcga gcgccgcgca cgacacgcgc gcctcgcttg ccgggcacga
      421 ggtgaaggtc gacattccat ccaatattcc gctcgtccgc gtcgatccgg tgctgctgca
      481 ccattgcctc atcaacctgc tcgacaatgc cgggcgctat gccgatccgg acacgccgat
      541 catcattcgg gcgcagcgca cgtccgatgc gatccttctg tcggtcatcg acgaaggacc
      601 gggcatcgcg ccgggcaatg aaaagcgggt gttcgaaacc ttcacccggc tcgaaggcag
      661 cgacagggca aagcatggca ccgggctcgg gctcgcgatc gtcaagggct ttgccgaagc
      721 gatggggctc gccgtcgagg cgggcaataa cgagcagccg cacggcgccc gcttcacgat
      781 ccagataccg ggcgcgctga tcatcgcgcc gatgagcgac aaggaaatcc catgaaggtg
      841 cgtcacaagg tcctggtcgt cgacgacgaa ctccacatcc ggcgcctcat ccatgcggcg
      901 ttggcgagga acgattatgc gatcgtcgag gccgagaccg cgcgcggggc gttggaacag
      961 cttcgcgccg agcgccccga catcaccttg ctcgatctcg ggcttcccga tcgcgacggg
     1021 ctcgaactgg tgccgctgtt caagcagcaa tccgacacga cgttgatcgt cgtgtcggcg
     1081 cgcgacgcga ccgaccaaaa ggtcgccgcg ctcgatctcg gcgccgacga ttatctcacc
     1141 aaacccttcg ataccgacga gctgcttgcg cgggtccggg tcgcgctgcg caatcggctg
     1201 actcgcgatg ggggcatgct ggcgctcgcg gtcgaggatg tgacgatcga cctcgtggcg
     1261 cgcaccgtga cgaaggcggg gaaggaggtt catctgaccc ccaaggaata tggcgtgctc
     1321 gcccagctcg cgaaatttcc ggggcgggtg atcacgcata agcagatcat ggcggaggtc
     1381 tggccgcgcg agtatgaaca tcatgtcgaa tatctgaggg tgctcgtgcg aaccctgcgc
     1441 cagaagctcg aaaccgatcc gcagcagccg cggatcatct gcaacgagct cggcatcggc
     1501 tatcggctgc ggcccggcaa tgatgcgtcg gcggaggatg gcgaaccgcc gcgctgaaac
     1561 atatgcaaac actatgggag acgctgccga tcgctctcga aatcaaatgg cgcgcctggc
     1621 tatcttcgtc cgatgacgca ggacgagatc gaggcgcagc cggacacgag tgagcggcga
     1681 atccgtgacc ggatggtcga ctggctgggc gcgcaaggtg cgctcttgct gacggtctat
     1741 atgtgcgcgg tcgtagcggc ggtcgcattc ctgctggaca ccgcccgcta gatttcgacc
     1801 ctagatttcg accagatcgc caatcctatg cgatctcaac gcctatccgc ttttcccgat
     1861 ctcgatttcc tatgcggccg tgcgtaattg agcggcctcc cgatggagga tcgaatgacg
     1921 aaatggcatg aaagcaccgc ggccaatatc ggcctgcgcg ccacaggtct gacgcttctc
     1981 gccgcggcct ggtggctggc ggtcaggctc caccatatgt cgcagactat tttgccgcgc
     2041 gacgcgacta cgccgataat tttgctctcg gccatccttt ttctctgcgc gagcgcgggc
     2101 agcgcattgc tgttcgtcgg accggcgctt tgggaaacgg tcgagatttc ggagcggtgg
     2161 cggcgcgcgc cgccctccgg atccgaagct gccgaagctt ttgatgggcg gccttcgcgt
     2221 acggtgcggc gcccggagga cggcgcaagc gcatagcaaa aaatctatgc gatcggcgct
     2281 ttgccgctct cgatttccta cgcggtgccg atctagttga gctgcctcgc tagccctcgc
     2341 cacccgcggc gacggcccga tcaagaggac aatatgaaaa gcatcatcat ccccgccgcc
     2401 ctcctggcgg gcctgtcgcc tgcttccgca ttcgcgcagg aagaggaggg caaggcggtc
     2461 acgctgtcgg gttcggcgac ggtcgcgtcg gactatcgct tccgcggcgt atcgcaatcg
     2521 gacaaggaaa tggccgtgca gggcgggctc accctgtcgc acgaaagcgg tctctatgcc
     2581 ggtgtctggg cgtccaattt ggccggctgg ggcagttttg gcggcgccaa tatggagcta
     2641 gacctgatcg gcggctacaa gctgccgatc tcggagacgg cgacgctcga tgccgggctt
     2701 gtctggtata tgtatcccgg cggtgccgac gaatccgact ttgccgaacc ttatgtgaag
     2761 ctgacgggaa cgtccggccc cgcgaccttg accgcgggcg tcgcctatgc gccgaagcag
     2821 cacgcgctcg ccaatgtgtc gaatgcgccg ggcagccgcg ggcaaagcga ggataatctc
     2881 tacctctggg gcgacggcgc ggtagcgatc gcgggcacgc cgttcaccgc gaaggcgcat
     2941 atcggctatt cggacggcaa cccggggctc ggccccaacg gcacgagcgt cgcaccgacg
     3001 ggccgctatt gggactggtc gctcggggtc gatacgacat ggcgtaacct gaccttgggt
     3061 ttgtcgtacg tcgacaccga catcggcggg gccgaggcgg cacgcctcca gcccaacttc
     3121 agcaaagggc tcgacggcac gggttcgatt gccgatgcga ccatcgtcgc gtcgataacc
     3181 gccgccttct gatcgatcgg ggctcggccg atcggccgag ccctttcgaa acggttttcg
     3241 gacgaagccg cgcgctacag gtccaccgtc aatctggcca tgacctgtcg cggctggata
     3301 tagacaaagg cattgtctcc cgccacttcc caaccatagg cgtcgaacag gttcgtggcc
     3361 tggacacgaa agacggcggc cgatcggccg atcgtgaacc gatggcgcag tccgatattg
     3421 atcgtcgtat agggcgaagc ttcgacaaga ttgagcctgt cggccgtgtt cggtccgacg
     3481 tgctcgacgg cggcatcgac cgatgtaggc gaagacggcg gccgccaatc gacgctggcg
     3541 atgatcttgc gcgacggcac gtcgatcggc cggttgccga tggcgcccga tgccacttcg
     3601 tcgcccttta cgcgcgcgcg aataagcgat ccgcccgcaa ccagcgtcaa tccgggcgtt
     3661 ggcgaaccgc tcagcgacgc ctctatcccg ctgtgtcgca ccacacccaa ccgccggaag
     3721 acattgcttc catcgacgcc gaaatagggc cgttcgatat cgaataggcc cgcgatcagc
     3781 gtcattcccg ctgcgcgcag cttcgcgccg gcatcgattt gccgcgtccg gatcgctggc
     3841 ggtgcttcat cgcggttcgc cgcggtttcg ggcgccacat cgctctcctc cagcccgcgc
     3901 gtgaaggaag cataaagcga tatcgcaggg gcaagatcga tcgtggcggt cgcgttgatc
     3961 agccacggtg tatcgcgtga ttcgggcgcg gggccgctcg gcgtggtcac ccttttgcta
     4021 tagtcgacct tttgcgcgcc gatgctgaat tgtccaaggc cgcgcacccg catttgatag
     4081 tcgaggccaa tcgaccattg gcgaacgcgg tcgctggatt gcgccgcgaa gtcgaagtcg
     4141 ggcctcgcga cataatcggc ttctccgaac ggcgcggacc ccagcgagcg ccggatcgaa
     4201 ccgccgtacc gccggtcctg agcgcgcccg cgcgcgacca gctggatgcg atgttcaaag
     4261 gcaccggtag agaaaccgcg cgcgacgccg agctcgccgc ttgtcgaaac gccggcccgg
     4321 cccggatagg cgctgacgat acgctcggcc ggcgttccca aaggcgcggc gcgcaggaag
     4381 atattatgcc cctcgcgcac ctcgttgtcc gaccggaaca gcccggcgcg caccgcaaag
     4441 ccggcgatgc tggtgcggcc catcgcgccg aaggtgtaac gctcggtctc gacgtcggcc
     4501 catttctggc cgaggaaccg tcgccgcgtc aggcggcccg gcgtctcgac gccattgccc
     4561 agtatgatcg gatagatgtc ctcatcggcg atgcgcgtgc ggcttccgaa cagctttaga
     4621 tcggttccgg gtgccggccg ccaatgcgcg atcgcggcga cattggtgac gtcggcgttg
     4681 ttccccgacg gatattcgct ccggctgaag ctcgcgccgc cgccgacact cagcacctcg
     4741 tttatcggca cggcggcgtc gatctcgacc agcgcgccgc cccagctatt ggcctggatc
     4801 accccgctca gcatcgcatc cttgccgggc gagcggaggc gatagtccac gacgcccgat
     4861 ggcgccggaa aggggtagct gaccgccgag ggcccgaccc tgatgcggtt gccttcgaca
     4921 agccggggcg agaagtcgcc ctgtcggtcg aaatacaggc cttccagccg cacattgccg
     4981 gcgtcgatcg gcgagaagcc gcgcaccgag ttcgggttgt agatgccgag cgtttcgccg
     5041 ccatcgttgg aaccgaacgc atcctcggcg tcggcgatcg cattgtcgtc cgcccgctga
     5101 gcgaacgctg gggcgtgggc gggtaaggcg gcaagcgaag cggcgatacc ggccatggtt
     5161 ttgaatcgtg tcattcacat aaggtcacac ggcgaaggat acttgacatc gcgcaagggt
     5221 tgctacgatc atgtgtgtat ggcgcacagc tcatatgatc tcccgccgct cgatggcctg
     5281 accgcgatgc tcgccgcggc ggaggaggga tcgttcagcg cggcagcggg gaaattgggc
     5341 cttacccacg gttcggtcag ccgccggatc gcggcgctcg agcattggct aggggcctcg
     5401 ctcttcgaac gccatggccg cggcgtccgg ctcacgccgg cggggctccg ctttgtcagg
     5461 gacgcccggc agagcctcgg cagattgtcg cacagcgccg agcaatggcg gccgcggcgc
     5521 ggccgtcaga cggtccggat gtcggtcgtc ccgtccttcg cgcggctctg gctgctgcgc
     5581 cgcctccctg ccatcgagcg ccacgatacg catatcgacc tgtcgctcga ccaccggatg
     5641 accgacctcg atagccggga agccgaagtg gtgatccgct atgggcgcgg cacttgggtt
     5701 ggagtcgacg cgcggcagat cgtcgaggaa tcgctcgccc cggccgcttc gtccgaactt
     5761 cgaggccgga tgagcgacgc gcccgcgatc gacgagctgc ttcaatggcc gctgctgcac
     5821 gactccgata catcgcagtg gcgggctttt ctgggcaggg cgggggtatc gtaccggccg
     5881 cgatggcagg atcgtcgctt cgaggattat gataccgtcc tcgccgcggc ggaggcgggg
     5941 ctcggcatcg cgctcattcg ccagcccctc gcggccgact ggctgatcga cggccggctg
     6001 acgacgatca cggcggagac catggacaat cccgcgagcc atttcgtctg cgtgcgcgcg
     6061 ggagaaacgc gtcaggccgt cctcaccctg cgcgaccgga ttctgggact cgcggccgga
     6121 gatggctctg gcaaatcgac gtaatcggcc gaccgcatcg cgctgcgccg ccgggaagct
     6181 accgggctac ggcctcgtgc gcaaacggcc ctcgtaatca cctttgccga tcgggacgcc
     6241 ctgcgtgcgc aggatgtcgt atgcggtgac cgcgtggaaa tagaagttgg gcagcgagta
     6301 ggtgaggagg agggtttccg gcgtcaaagc gaaggtccgc ggcgcccagc tcgacgtcga
     6361 agcattttcc tcatccagcg gccgaaaaag ctcgatgtcg aggttcctgc cggaccagct
     6421 attgacttcg tcgggggtca gcgcttccag cgcggctacc gcctggacga tcgtggattg
     6481 cagctcggcg aacggcatct cgccgacgag cgggggcgga gcgaacgcgc cggtcttcgc
     6541 cgcgttcaat ccccaaaccg catgattttt cagcgcttcg atctggaaat gaaagggtgc
     6601 catgtcgggg cagaggcggg cgccgacgaa gtcgtcggga tcggtgccgg tttccgcgcc
     6661 atgtcgggcc gcacggtcga gccaacccga taccgcgcgc agggtttgca gaaaggtcgg
     6721 cacgcttagg tcgaagagcg aaatggtcat ggcttactat agctgccgga aagcggccgg
     6781 caaatatccg tgagcttatg aacctatgac ccggcggtcc cgggtttctg ctccttatcc
     6841 ccgggaagac accacagcgg gttcaggccg caaagccgtt ccagcatcgg tgcatattcg
     6901 gtcttgatcg cggccatttc cttttcgccc agatgctgga cggcaaatcc gtgcggcccc
     6961 tcctggatga cgaaggtcag cggattgatg cccggcttca cgccgccaag cgcctggttc
     7021 gatacgttcg gcgcatagtg catgatatgc gggttggtcg acgtctcgac ctctcccgac
     7081 ctttcgggat cgatataggt ccgcaggatc ggagagagca tgtacgacac gccgccgtga
     7141 tccggcgcct tgtaatggcc ggacgcatag cgctccttga tcagcttctt gagatcggcg
     7201 ggcggcgttc ccttcgcctg aagctccgcc gcgtcgaaga agacgcgcat attggccttc
     7261 gatccgacct cgtcgaacgc gatcggatag atgatgtcgt tccgatacgc cgtgaacggc
     7321 caatcgccgc gaaaggcatc gtcgccggtt cgcgcgagaa aggcatgaaa accgttggtt
     7381 cccgcgcgcg cgatttcaaa gcccttgcgc gggttcagaa catagatcgt ggcccgctcc
     7441 tgcagatgct tcggcagcgc gctctccgcc aactgaatct cgaggtcgcg gggcaaatgg
     7501 aaggaatcga ccgtagcgcc ggcccgcgcc tccgactgcg ccgattgacg ctggtcctcc
     7561 ccggcgcccg gctgttcggg tgaacaggcg gcgacgatca gcgacgccat acagaccgcc
     7621 ttcagatgcc atttcatcat tgctggctcc ttgcttgccc gcgcgccgcg tggcggtgcg
     7681 ggctccgtag agattagttg ccgaggtcgt ccgaaaagcc ggctgcgagg cgctgccact
     7741 gggcgatggg ccgcccatcg ctgcggaagg ggtgcacctt ttcattttct ccccagcctt
     7801 gcggccagcc ggggggcgag acttcctgct cctcctgtcg cccatagacg gtgaggtcga
     7861 gcaacctgta gctattgtcc atcgcctcga ccccgcggcg cgtcgtccaa taggtctcga
     7921 agacatcgga gccgcggcgc agatagcaga tgaggtacat gctgcccgcc tcgcggccgg
     7981 ccagcagggt gtcgagcccc tcggtcaccg aataccatgg catgtcccat cccatgaaat
     8041 cgcggtaccg gacgctctct tcatacggcc cctggcagaa ggtcgcatag gtcgcgtcgc
     8101 gcgaatggat gtagctgagc tcgcgcacct gcgaggtgaa ccagctgcat ccctggcatt
     8161 gcctttccgc gggccggccg gtgaaccaca tataataata ggcgatcagc atgcgccggc
     8221 cctcgaacgc gtcgagcagc gtcgtggggc cgtccttgcc gatcagcggc gcggccccgt
     8281 cgaccgccac catcggaagc cggcgccgcg tcgcggcgat ttcgtcgccg acgcgcatgt
     8341 gcgccttttc gcgcaggcgc aattcgtcca gctcggcctg aaacgtcgcg cggtccgcga
     8401 ccttcggcgt ggtggattca ctttccgata tgtgcatctt tctctcccga tttcgtcgtc
     8461 gcgttatcgc ccgggaccat ggattgcagt cacgcgccga gccgcgcgaa ataggcccgg
     8521 atcattcgac ctcttctgat ccgcatcccg atgcctcgtc gcgacagccg gcacgcatca
     8581 tgccgatggg aagggggccg agggcggcgt cgccagcgtc accagattgc cgtcgggatc
     8641 gcgcacttgt gccaaggtcg aataatcgcc cgtgatgtcg tcgccgagcg cgatcccggc
     8701 gctttcgagc cgccggcgct ccgccgcaag atccgcgacg taaaggaaga tcgaaccccg
     8761 gtcggcaatc tcgtcactgg tcgaaagcat gagcccgcct tcgtcgaaca attcccagtg
     8821 cagcaaagtg ggcatcggcc ggtgatcggg gccgcggccg aacaggtccg tataccagcg
     8881 ctcggcagcc gcgagatcgg cgacgagcaa tgaggggtag gttttctcca ctttcatttt
     8941 gggtctctcc ggcttttctc acctgcgagc gcggcgcggg tttcgcatca tactacctga
     9001 tgagacgatc ctggttccgt gcataggccg atcaggacat gtgtgcatgg cgcacatgac
     9061 ctgcggctgc ggcttggctg cacccgatgg cctcatatat cctgttcgtc gctgatcgcg
     9121 ccgatccgcg gaacgacctg cgaccagccg ccgctcatat tgcgataggc gctgtcgttc
     9181 tgcggcagta cgaagcccga atggacaagg cgcaggcgcg tgccgccatc ggcgggttcg
     9241 agcgtgaagg tgacgatcgt gtcgagcggc gcgccatagc cgacattctc ctcatgcccg
     9301 ccgcgccatg aatagacgaa acgctcgttc gggattacct ccagcacctc gcattggatc
     9361 gttccgtccc acttgccggc ggccgtcgtc cgataggtga agcggttgcc gaccaccggc
     9421 gcgaagcccg ccggcgccat gcccagccag cgccccatca gctcgccgga ggtcagcgtc
     9481 ttccagatcg tctcgggcgc atggaggaaa atctcgtcga cgacgatctc ccgggcattc
     9541 gccggcgctt caccgtcatt cacgggtcga tctcctgcaa gagcgcgcgc aggcccacga
     9601 agctgtcccg ccagaaggaa tcatagtggg cgagccagtc gagcagcggg ctgagccctt
     9661 caggccgcgc gcggtaatag acattgcggc cctcagggcg ctcgacgacg agcccggcct
     9721 gcttgagcgc cttcagatgc tgcgagaccg caccctgcgt caccccgctg acgcgggtca
     9781 gcgcggtcac attgacctcg tccgagcgcg caaggcgctc gaacaccgca cgccgggtgg
     9841 gatcggcgag cgcgcgcaat acggtatcga cggcgacggt ctgcatcatg cagactgatt
     9901 agcagatgct aatcaattag caatgactaa ttggttagca tgccgaacaa cgctttttgg
     9961 gaggcaacgc aaagaccggg gcattcggat ctctaaatga tcttgtgttc gggcgcgttg
    10021 aggtcgatcc tctccattcc agcgcgcctt ttccggtgag gaaaataggc aggcaggagg
    10081 agagcgatat ggatgggact atccacggca ccagagccat cgcgatcgtc ggacccgccg
    10141 gcacaggcaa gacgagccta gccgaagcca tgctgttcgc ggcgggggcc agcgcccggc
    10201 tgggcgcggt cgat
//
LOCUS       DATMSU010000075        17548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_105800,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000075 DATMSU010000000
VERSION     DATMSU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 17548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17548
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105800"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VN034_02000"
     CDS             <1..1386
                     /locus_tag="VN034_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWV59398.1"
                     /translation="VAAASAAAPPATTAAAGKPNILFVLVDDMGFGDLSVMGNQKAQT
                     PNLDRLAREGVLMTQFYDAAPICSPSRAGFLTGRFPAEVGFINYVSDRAYNRSVGQAD
                     WLDPKLPNIARLLKGAGYDTAHIGKWHLGGGRDIGDAPWPTAYGFDHSFTTFEGLGPR
                     VLVSDEERFLAEQSDKLGEGPRFWELKTNLTGLYADMTVDFVAQHSERPWFVNLWLND
                     MHDPWAPDDESLSEVMGKGTSSDDDRMLASLVKMDKTLGRLFDRLDQMGEMDNTLVIV
                     TSDNGPSSSPRYYKDGQVAPGSTENLRGRKWSLYEGGIRQPLILYWRGRAKAGYRDEQ
                     TVGQGVDLLPTLASIVGARIPAGTDGVDLSPVIEGRAIPQRPDLYWAYGKEGAAKEPP
                     QPALEHDQAPPFAIREGNWKLLAEAGGASPQLFNLARDPGEANDVAASEPAVRDRLLA
                     KLKAWMATLPR"
     gene            complement(1468..4296)
                     /locus_tag="VN034_02005"
     CDS             complement(1468..4296)
                     /locus_tag="VN034_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59399.1"
                     /translation="MKKQTGFSAAVSVIALVASVPAWGQSTDAGGTVASDTLADAPPE
                     STEDAIVVTGVRRSLERAADIKRESVQVVDAIVAQDIGKLPDPTTAAALQRVPGVQVS
                     VNRNNELGDVRVRGLPDVLTTVNGREVFSTIGRNFDLQDMPAEALSRVNVFKSQTAEL
                     TEGGLAGVIDLQLNRPFNFRDPTVVLGGRANYGDRVDKINPQFSLLATDRWDTGIGEI
                     GALINGTYSKSDYYRASTVLFQRRSTNTAPFNTPGYVIPGILQNFPQQGFIERHQFNG
                     ALQWQASPSLQVYAEGFYTYFRDRGSRNGANIQPFTNGTTITDVTPGTSCITTYVRSN
                     GNNPVIQTDAEGNKSWTLTPAQLANPVELCQPESMTFANLVSNQTTQARDLNQRNKQF
                     AGGLKYEEGRLNAVLDVAYQTSKYELQNITSDIGQRLPSLTVTMDNDGHAEYTVPGDA
                     LLSRDNLYIRNALIQNFTENRGKLFQAKGDVDYDLDGFLSNIAVGVRYAHRSAEEQAV
                     NLNTATPGGNIGTGSEATAVLVSDTGLPDDFLVLGSRAPDLNNGSRFYIPNPDFLLSE
                     DGLDAARAYFGLPAGRPDYDPGRAFDAKEDTLAAYVQGKYETDLSNSITLDGVVGVRY
                     IRTSRTIGTFVEAEGGEYDRLTADSVDHDWLPNATARFRIGDNIQIRFGYAKSLRRPN
                     FEALNPAITLNQSNNPLVQSTGNAGNPDLKAQKSESLDATFEYYFPSGYVAIAGYYRD
                     ITDRVITSGAVEMIDGVDYSVARPRNVGQATLKGIELSTQYFLDFLPGALSGLGVQGA
                     FTLADSKVGGDDPLAGYPLQGVSKYNYTVGLLYEKSGVNARLVYTYRSKYLALDATGQ
                     PTLRPMTEDTIASLEVPALVTYARGGGRLDFNIGYDITDQIRVDVGGTNILGNNTSTY
                     YDYPGYTNINNEMSYDETTYSVGVRVKF"
     gene            4511..6235
                     /locus_tag="VN034_02010"
     CDS             4511..6235
                     /locus_tag="VN034_02010"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWV59400.1"
                     /translation="MNVRFAARLLARAGCAVAALATAALPSAAIAAEAEAASARPQPT
                     RPNVVVILLDDVGFSDFGSFGGEIPTPNIDALTKNGLAFTQFYNNARCSPSRASLLTG
                     TYPHQAGLGHLESVHVPGSKGLHSKLSDRVVTLAEVLKSAGYFTAMAGKWHLGISRGV
                     GPWQRGFDRSLTSPSGELYYRDQPQPLAKSVYIDGERVPASSPRVGQGYWYSSDMFID
                     WQTKFIGEARDQRKPFFLYMPFVGAHFPLMAPPEDVAKFKGRYMRGWEVIRRERFERQ
                     KKLGIIAADAELPAALPGSYDWNKLSAEEKDRFDTMMAVYAAVIHRVDRSIGTLVERL
                     RQSGELDNTLILLMSDNGGTAESGPDGRLKGEGLPGTAQSVVWTGMNWATLQNAPFQY
                     YKHHTYEGGIATPLIAHWPRGIAAKARGTLVREPGHLIDVMPTLVELAGATYPKSFNG
                     HTILPMEGRSMVPAFRGEALTRGKPIFWEHEGNRAVRDGKWKLVAGFEKPWQLFDMTT
                     DRTEMHDLAASQPDRVRNMAGQWDAWAARSYVDAWSEAYDPHLKGKARRIWGGADVPQ
                     LPQALVGK"
     gene            complement(6256..7653)
                     /locus_tag="VN034_02015"
     CDS             complement(6256..7653)
                     /locus_tag="VN034_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWV59401.1"
                     /translation="MSRLIVVSNRVSVAKGRGAAGAQGGLAVAMSAALHKRGGIWFGW
                     SGNEVEQFTGQLTMERADGFTTATVDLEPHDVDEYYNGYANRTLWPLFHYRIDLAEYE
                     NEFGRGYERVNEHFANSLYPLIEEEDVVWVQDYHFLPLGERLRAQGVRNRIGLFLHIP
                     WPPTRLLVSLPHHERLVAAMLHYDVIGFQSVEWLESFLHYCRKEHGAEVDEDSGTVRI
                     GNRSSIARAFPIGIDHAEFMALGETEIAKRSQQRLGGSARKRTIAIGVDRLDYSKGLP
                     ERIDALERLFSSHDDMVNELLFVQIAPPSREDVRSYQQIRATLEQKTGQFNGAHSDVD
                     LVPIRYVNRGFDRAELFGFYRAAKIGLVTPLRDGMNLVAKEYVAAQDPDDPGVLILSR
                     FAGAAEQLTDALLVNPHDGGEVSRAIWTAMHMPLAERKARWEKLFATVRDADVIGWAD
                     DFLSALTQDPALPEA"
     gene            complement(7650..9431)
                     /locus_tag="VN034_02020"
     CDS             complement(7650..9431)
                     /locus_tag="VN034_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWV59402.1"
                     /translation="MSNLSLWPIGNCQVSALIDSAAGLVWGCVPRVDGDPVFCALMND
                     DRQDEGVWRFALDGQVRATPRYSRNTPILVTRLEAEDGSAVDVIDFCPRSERSGRMYR
                     PVAYARIVRPVAGSPRIRMKLAPMRDHGAERAPTTRGTNHIRYLVGNQPLRLTTDAPV
                     GYVMEELAFRVESDLHFFLGPDEPFTGNVRESVRGMEEATGQYWRSWVRGLATPMDWQ
                     DAVIRAAITLKLCQHEETGAIVAALTTSIPEAPHSERNWDYRYCWIRDAYYTVQALNR
                     LGALDVLEKYLAYLRNIVDRAAGGQVQPLYSVMGVAELDEGVAEDLGGYRGMGPVRIG
                     NAAYQQVQHDAYGQIVLPTVQGFFDQRLFRMADERDFESLESVGEVAWAMHDQPDAGL
                     WEFRTKQLVHTYSAVMSWAACDRLGNVAARIGKPKREAYWNDRAAKIRETIEREAWDE
                     AAGHYGAAFGHAHLDASLLQMVELRYIRADNPRFQATFAAIEKALRRGEHMLRYDSED
                     DFGLPETAFNFCTFWLIEALHLCGRSEEARELFEKMLAHRTPSGLLSEDLDFENGELW
                     GNFPQTYSLVGIIHCAGLLSRGWDTVR"
     gene            complement(9428..10096)
                     /gene="otsB"
                     /locus_tag="VN034_02025"
     CDS             complement(9428..10096)
                     /gene="otsB"
                     /locus_tag="VN034_02025"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWV59403.1"
                     /translation="MALFLDFDGTLVDIAETPDAILVPDDLVRRLEVLAARVDGRLAL
                     ITGRSLSDIGGHLGRGAIRIVGSHGAEHGDAAAPAATLSSDTKMAIASLAAQWPGLLV
                     ETKPHGIAIHYRQEPHAEAAVFTAMDDIAEREGLAVRRGKMVVELGPVQANKGRAVAR
                     LMHEPPYVGATPVFIGDDVTDEDGFAAAAAAGGHGILVGAPRPTAAHYRLSSTAEVYR
                     WLGL"
     gene            complement(10225..10557)
                     /locus_tag="VN034_02030"
     CDS             complement(10225..10557)
                     /locus_tag="VN034_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59404.1"
                     /translation="MAIVSASPQPLGAYRIARQSLALGVPLAPNQVYRILDRLGGRVR
                     RVESLNAYFEADGQPGAMTICRGCGRTRTLDAAYDAEIDRLCRAAGFRPNRVIVEVIG
                     LCADCRGK"
     gene            complement(10658..11026)
                     /locus_tag="VN034_02035"
     CDS             complement(10658..11026)
                     /locus_tag="VN034_02035"
                     /inference="COORDINATES: protein motif:HMM:NF015180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HWV59405.1"
                     /translation="MTTNLVEGTAVSASLLCLGHCLALPLILLALPMLAGTFFESELF
                     HIVAAALVVPAAALAFILGYRRHRALLPAQLGSAGVACIVAALFPAWGEPASAGITAI
                     GSLFLIGGHLINWRLRRTLA"
     gene            11140..13230
                     /locus_tag="VN034_02040"
     CDS             11140..13230
                     /locus_tag="VN034_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59406.1"
                     /translation="MKFRVLLLSAAVPAVVPHIAYAGESVPAAKPAAGPDAGFMQAGS
                     TADPEIVVSANVLGLRADETATPVITLTGEELIHRRQATLGDTLAGQPGINFDNFGGG
                     ASRPVIRGQTAPRVQALSDGASVHDASAISPDHAVTTEPLLLRGIEVLRGPSALLYGG
                     SAIGGAINLLDEKVPTSIPDGGIAGTVEGRFGTADDERSLVGGITIGGQGLALRVEGV
                     HRSSNDYRVPKGVGERHVDGSYNDTSTFTVGGSWVGSGGYLGVAYTRQRSEYGLPGHS
                     HDYESCHPHGSSLHCGGHGHDDEDHDHDHDHDHEHEEVPFVKLRSERFDIRSEYEDPL
                     PGFEKVRFRLSFTDYEHDEIEHNEVATTFSNKAHELRFELAHKPIGPLRGAFGVQHSE
                     SQFSAVGEEAFLPESETRNTALFLMETLDAGPVRFELAARQEWQRIETTLNREVSHRP
                     FSISGAAIWDIGGDYSLALSLARSQRAPNVQELYARGIHLATNTYELGTATLGKETGK
                     SIDLTFRKTKGATTFTIGAYHQDFDDYIFANTLDQFEDFRLIRYTAADATFTGVDGEV
                     RHDFGTVGVSLFGDYVRAKFKGDLGNLPRIPAGRLGARADGRWGALSGDVEYYHVFEQ
                     DRIAAFETRTSGYDMLNATLAYKLALGPKADAELFVRATNLTNELAYNHASFIKNASP
                     LRGRNFVFGLRTSF"
     gene            complement(13294..14082)
                     /locus_tag="VN034_02045"
     CDS             complement(13294..14082)
                     /locus_tag="VN034_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59407.1"
                     /translation="MSMIFDGRERRSASGLFQEIEALQDGQAWGKLLDAGTGTHSIGW
                     IGSLATECWTAVSGAEAHAAQVADAVAAKRRAADRIIVGNWADPALLAGERYDTVLAD
                     YLLGAIDGFAPYFQHRLFARLRPHVGRRLYVVGLEPYVSGEPDSEAGRLIWEIGRFRD
                     ACLLHAGEQPYREYPAQWTVDHLEASGYRVIAAKRFANRYKERFVNSQIDMCAPRLAR
                     IADRGLADALEMRGEALRAEALAHVAREDGLRHGFDYVLAAEPA"
     gene            complement(14168..14944)
                     /locus_tag="VN034_02050"
     CDS             complement(14168..14944)
                     /locus_tag="VN034_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59408.1"
                     /translation="MLRLNPAPATPYVAQLNIGVGRRSLAIGLAVAIAGLMLLMLLSW
                     GPGAQPDAEEESISVVSLEATEIAKDAPEPASPERERAEERTTSPQPEPDEPQPLEPL
                     PPQVPEPKVAPPIAMPVQRPLPASPPKAARPQGGQVYGPPNTGGSPSSRDSERVGTAP
                     NGEALYAAAWYREPSDGELRGYLSTASGPGWGLIACRTAPDYRVEDCVGLDEYPYGSQ
                     INRAVLAAAWQFQVRPPRLGGRPMVGSWVRIRIDYGTRRR"
     gene            complement(14982..15056)
                     /locus_tag="VN034_02055"
     tRNA            complement(14982..15056)
                     /locus_tag="VN034_02055"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15022..15024),aa:Asn,seq:gtt)
     gene            complement(15139..15909)
                     /locus_tag="VN034_02060"
     CDS             complement(15139..15909)
                     /locus_tag="VN034_02060"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HWV59409.1"
                     /translation="MHRKIALVASNAPAAMEAEAELRPLYDFVDLTEADLLIALGGDG
                     FLLHMLHQLLDQRRSLPVFGMNRGTIGFLMNEFRVEGLLDRIAAARPYLIHPLSGDIT
                     TISGERHILPAINEISLLRETRQAAKLEVMINEKTMLEELACDGVLVSTPAGSTAYNL
                     SANGPILPLDSAMLALTPISPFRPRRWRGALVPESTSIRFNVREAAKRPVSAVADQRE
                     IRDVKTVLVTTDRSRPLTLLFDPDQGLDERIAMEQFIV"
     gene            complement(15953..16975)
                     /gene="moaA"
                     /locus_tag="VN034_02065"
     CDS             complement(15953..16975)
                     /gene="moaA"
                     /locus_tag="VN034_02065"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWV59410.1"
                     /translation="MSVAVPLSHRAASRAPLIDGVGRRISYLRLSVTDRCDLRCRYCM
                     AEDMTFLPKSAVLSIEEMAELATRFVARGVRRIRLTGGEPLVRRGIDTLAMRLGALIG
                     HGLDELTLTTNAMRLAEHAPMLAAAGVRRINVSLDTLDPAAFRHITRVGDLDVALRGI
                     DAARAADLAIKINMVALAGLNEDQLLPMLDYCAATGCDLTLIETMPLGEVEGDRSDHY
                     IPIHHFIAPLRETRAIYPVDKRTGGPARYFAVEGSLVTLGLITPLSDNFCATCNRIRL
                     TVEGRIYMCLGQDDHVDLRAALRDGGDVDGLIDAALAGKPRAHDFHIERESKPAVARH
                     MSATGG"
     gene            17029..>17548
                     /locus_tag="VN034_02070"
     CDS             17029..>17548
                     /locus_tag="VN034_02070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWV59411.1"
                     /translation="MNRLSESLSACVRKLEALHARHDADAGVIFVQVDQMARINNSAG
                     TATGDSVLDEIGRRLENFASDEFGQGSCVDRLDGPRFLIVPATPLSLGALRAQERALH
                     VALAEPIVGDPNGRLSIRIAAALITRDESVAEQLRAAGDQLARPASARDGVMVHAALS
                     RNEVVIHYQPQYD"
BASE COUNT         3092 a         5578 c         5814 g         3064 t
ORIGIN      
        1 gtggcggccg cctcggcggc ggctccgccc gcgaccaccg cggcggcggg caagcccaac
       61 atattgttcg tgctcgtgga cgacatgggc ttcggcgacc tgtcggtgat gggcaaccaa
      121 aaggcccaga cgccgaacct cgaccgtctc gcgcgcgaag gcgtcctgat gacgcaattc
      181 tatgacgccg cgccgatctg ttccccatcg cgcgcgggct tcctgaccgg gcgttttccg
      241 gccgaggtcg gcttcatcaa ctatgtcagc gaccgcgcct ataatcgatc ggtcggccag
      301 gccgactggc tagaccccaa gctgcccaat atcgcgcggc tgctgaaggg cgccggctat
      361 gataccgcgc atatcggcaa atggcatctt ggcggcggac gcgacatcgg cgatgcgccg
      421 tggccgacgg cttatggttt tgaccatagt ttcacgacct tcgaagggct cgggccgcgc
      481 gtgctcgtct ccgacgagga acgcttcctc gcggagcaat cggacaagct gggcgaaggg
      541 ccgcgcttct gggaattgaa gaccaacctc accggcctct acgccgacat gacggtcgac
      601 ttcgtcgcgc agcattcgga aaggccgtgg tttgtgaacc tctggctcaa cgatatgcac
      661 gatccctggg cccccgatga cgagagtctg tccgaggtga tgggcaaggg cacgtcctcc
      721 gacgacgacc ggatgctcgc gtcgctggtc aagatggaca agacgctcgg ccgcctgttc
      781 gaccgcctcg accagatggg cgagatggac aatacgctcg tcatcgtcac cagcgacaac
      841 ggtccgtcat cgtcgccgcg ctattacaag gatggccaag tcgcgccggg cagcaccgaa
      901 aatctgcgcg gccgaaaatg gagcctatac gaaggcggca tccgccagcc gctgatcctc
      961 tattggcgcg gccgcgccaa ggccggttat cgcgacgagc aaacggtggg gcagggggtc
     1021 gacctgttgc cgacgctcgc gagcatcgtc ggtgcgcgaa tacccgccgg caccgacggg
     1081 gtcgatctgt cgccggtgat cgagggacga gcaattccgc agcggcccga tctttattgg
     1141 gcttatggca aggagggcgc ggcgaaagag ccgccgcagc cggcgctcga gcacgatcag
     1201 gcgccaccct tcgcgatccg cgaaggcaat tggaagctgc ttgccgaagc gggcggcgca
     1261 agcccgcagc ttttcaacct cgcgcgcgat ccgggcgagg cgaatgatgt cgccgcgagc
     1321 gaacccgcgg tccgcgaccg cctgctcgcc aagctcaagg cgtggatggc gacgcttccc
     1381 cgataagaaa atggccccgt cgcacggagc gcggggccga ccgaatgaca ggagcgcggc
     1441 ggcaatcact gccgccgcgc cttcgctcta gaatttgacg cgcacgccca ccgaataggt
     1501 cgtctcgtca tacgacatct cgttgttgat attcgtatat ccgggatagt cataataggt
     1561 cgacgtgttg ttcccgagga tgttggtgcc gccgacgtcg acacggatct ggtcggtgat
     1621 gtcatagccg atgttgaaat cgagccgtcc gcccccgcgc gcataggtga cgagcgcagg
     1681 tacttcgagc gaagcgatcg tgtcttcggt catgggccgc agcgtcggtt gaccggtggc
     1741 gtcgagcgcc agatatttgg agcgataggt atagaccagt cgcgcgttga ccccgctttt
     1801 ttcgtacagc aggccgacgg tgtagttata tttcgacacg ccctgcagcg gatagcccgc
     1861 cagcggatcg tcgcccccga ccttggaatc ggcaagtgtg aaggcgccct gcaccccgag
     1921 ccccgacagc gcgccgggca ggaagtcgag gaaatattgg gtgctgagct cgataccctt
     1981 cagcgtcgcc tggccgacgt tgcgcgggcg cgccaccgaa taatcgacgc cgtcgatcat
     2041 ctcgaccgcg ccgcttgtga tgactcgatc ggtgatgtcg cggtaatagc ctgcgatcgc
     2101 gacatagccg ctagggaaat aatattcgaa ggtcgcgtcg aggctttcgg acttctgcgc
     2161 cttgaggtcg gggttgccgg cattgccggt gctctggacc agcgggttgt tcgactggtt
     2221 gagcgtgatc gccgggttga gtgcttcgaa gttcgggcgg cggagcgatt tggcatagcc
     2281 gaagcggatc tggatattgt cgccgatccg gaaacgcgcc gtcgcgttgg gcagccagtc
     2341 gtgatcgacc gagtctgcgg tcaggcggtc atattcgccg ccctccgcct cgacgaacgt
     2401 gccgatcgtg cggctcgtac ggatatagcg taccccgacg acgccatcca atgtgatgct
     2461 gttcgacagg tcggtttcat atttgccctg cacataggca gcgagcgtgt cctccttcgc
     2521 gtcgaatgcg cgccccggat catagtcggg ccgccccgcg ggcaatccga aataggcgcg
     2581 cgcggcgtcg agcccgtctt ccgagaggag gaaatcgggg ttgggaatat agaagcgcga
     2641 accattgttg aggtcgggtg cgcgcgagcc gagcacgagg aaatcgtcgg gcaggccggt
     2701 atcggagacg agtacagcgg tcgcctcgct ccccgtcccg atattgccgc cgggcgtcgc
     2761 cgtgttgaga ttgaccgcct gctcttcggc cgaacggtgc gcatagcgaa cgccgaccgc
     2821 gatattcgac agaaagccat cgagatcata atcgacgtcg cccttcgcct ggaacagctt
     2881 gccgcgattc tcggtgaaat tctggatcag tgcgttgcgg atatagagat tgtcgcggct
     2941 gagcagcgca tcgccgggca ccgtatattc ggcgtggccg tcgttatcca tcgtcacggt
     3001 caggctaggc agccgctggc cgatgtccga ggtgatgttc tgcagctcat atttcgaggt
     3061 ctgataggcg acgtcgagca ccgcgttcag ccggccctcc tcatatttca gcccgccggc
     3121 gaattgcttg ttgcgctggt tgaggtcgcg cgcctgcgtc gtctggttcg acaccagatt
     3181 ggcgaaggtc atgctttcgg gctgacacag ctcgaccggg ttcgccaatt gcgcgggggt
     3241 cagcgtccac gacttgttgc cttcggcgtc ggtctggatg accgggttgt tgccgttcga
     3301 gcggacatag gtggtgatgc aactggtgcc cggggtgacg tcggtgatcg tcgtgccgtt
     3361 ggtaaagggc tggatgttcg cgccgttgcg gctgccgcgg tcgcggaaat aggtgtagaa
     3421 accctcggca tagacctgga gcgacggcga cgcctgccat tgcagcgcgc cgttaaactg
     3481 gtgccgttcg ataaagcctt gctgcgggaa attctggagg atgccgggga tgacatagcc
     3541 cggcgtgttg aacggcgcgg tgttggtgct gcgccgctgg aacagcaccg tcgaggcgcg
     3601 ataatagtcg gacttcgaat aggtgccgtt gatcagcgcc ccgatctcgc cgattccggt
     3661 atcccagcgg tcggtcgcga gcaggctgaa ctgcggattg atcttgtcga cgcggtcgcc
     3721 gtaattggcg cggccgccga ggacgacggt cgggtccctg aaattgaacg ggcggttgag
     3781 ctgaaggtcg atgaccccgg caagcccgcc ttcggtcagc tcggcggtct gcgacttgaa
     3841 aacattgacg cgcgacagcg cctcggcggg catgtcctgc aggtcgaagt tgcggccgat
     3901 ggtcgaaaac acctcacgcc cgttgaccgt cgtgagcacg tcggggaggc cgcgaacgcg
     3961 cacgtcgccg agttcgttat tgcggttcac cgacacctgg acgccgggca cgcgctggag
     4021 cgcggcggcg gtggtggggt cgggaagctt gccgatgtcc tgcgcgacga tcgcgtcgac
     4081 cacctgcacc gactcgcgct tgatatcggc ggcgcgttcg agcgagcggc ggacgccggt
     4141 gacgacgatc gcatcctcag tggattccgg tggcgcgtcg gccagggtgt cgctcgcgac
     4201 ggtgccaccc gcgtcggtcg actggcccca cgccggcacg ctcgcgacga gtgcaatgac
     4261 cgacacggcg gccgaaaaac ctgtctgctt cttcatcttc cctctcccaa gtccgtgcgc
     4321 aggcattttt tgcccttggc gtcggcaaac aggggcgggt cgtcgatgag tcggcgcggc
     4381 actcggccgc aaacctcact ccacccggct ctccgcaccc ttttcgtcga cgcttcgtct
     4441 gggacgttga ccaacggacc aaacaaagtc aatatatata tgataaatag gtgaataggg
     4501 agagtgcccg gtgaatgtca gatttgctgc tcgtctgttg gcccgtgcgg gctgcgccgt
     4561 cgcggcgctc gcaacagccg ccttgccgtc ggccgcgatc gcggccgagg ccgaggcggc
     4621 atcggcccgc ccgcaaccga cccgtccgaa tgtcgtggtg atcctgctcg acgatgtcgg
     4681 cttttccgac ttcggcagct tcggcggcga aatccccacg ccgaatatcg acgcgctgac
     4741 caaaaacggc ctcgccttta cccaatttta caataatgcg cggtgcagcc cgtcgcgggc
     4801 gtcgctgctc accggcactt atccgcatca ggcggggctg gggcatctcg aatcggtcca
     4861 tgtgccgggc tcgaaagggc tgcacagcaa gctttccgat cgcgtcgtca ctttggccga
     4921 ggtgctgaaa tcggcgggtt atttcaccgc gatggcgggc aaatggcacc tcggcatatc
     4981 gcgcggcgtc ggcccttggc agcgcggctt cgaccggtcg ctcacatcgc cttcgggcga
     5041 actctattat cgcgatcagc cccagcccct cgcgaaatcg gtttatatcg acggcgagcg
     5101 cgtgcccgcc agctcgcccc gcgtggggca gggctattgg tattcgtcgg acatgttcat
     5161 cgactggcag acgaaattca tcggcgaggc gcgcgaccag cgcaaaccct tctttctcta
     5221 tatgcccttc gtcggggcgc atttcccgct gatggccccg cccgaggatg tggcgaagtt
     5281 caagggccgc tatatgcgcg gctgggaggt catccgccgc gaacggttcg aacggcaaaa
     5341 gaagctcggc atcatcgccg ccgacgccga actgcccgcc gcgctgccgg ggagctacga
     5401 ctggaataaa ttgtcggcgg aggagaagga tcggttcgac acgatgatgg cggtctatgc
     5461 cgcggtgatc caccgcgtcg accggtcgat cggaacgctc gtcgagcgtc tgcggcaatc
     5521 gggcgaactg gacaatacgc tcattctctt gatgagcgac aatggcggca ccgccgaaag
     5581 cggccccgac ggccgtctca agggcgaagg cctgccgggc accgcgcagt ctgtggtctg
     5641 gaccggaatg aactgggcga cgctgcagaa cgcgcccttc caatattaca aacaccacac
     5701 ctatgagggc gggatcgcga cgccgctgat cgcgcattgg ccgcgcggga tcgccgcgaa
     5761 ggcgcgcggc acactcgtgc gcgagccggg gcatctgatc gacgtcatgc cgacgctcgt
     5821 cgagctcgcc ggcgctacct atcccaaatc cttcaatggc catacgatcc tgccgatgga
     5881 gggccgttcg atggtccccg ccttccgcgg cgaggcgctg acgcgcggca agccgatctt
     5941 ctgggagcat gagggcaacc gcgccgtccg cgacggaaag tggaaactcg tcgccggctt
     6001 cgagaagccg tggcagcttt tcgacatgac caccgaccgc accgaaatgc acgacctcgc
     6061 ggcgagccag cccgatcggg ttcgcaatat ggccggccaa tgggacgcct gggcggcgcg
     6121 cagctatgtc gatgcgtgga gcgaggctta cgacccgcat ctcaaaggga aggcacgccg
     6181 gatctggggc ggcgcggacg ttccgcaact gccgcaggcg ctggttggga aatagcgtct
     6241 gccgcccggc ggctcttacg cctcgggcaa agccggatcc tgcgtgagcg ccgagaggaa
     6301 atcgtccgcc cagccgatga cgtcggcgtc gcggacggtg gcaaagagct tttcccagcg
     6361 cgccttgcgc tcggcgagcg gcatgtgcat cgcggtccag atcgcgcgcg atacctcgcc
     6421 gccgtcgtgc ggattgacga gcagcgcatc ggtgagctgc tcggccgcgc ccgcgaagcg
     6481 cgagagaatg agcacgcccg gatcgtcggg atcctgcgcg gcgacatatt ccttcgcgac
     6541 gaggttcatc ccgtcccgaa gcggggtgac gaggccgatc ttggcggcgc gatagaagcc
     6601 gaacaattcg gcgcggtcga agccccggtt gacgtagcgg atcgggacga ggtcgacgtc
     6661 cgaatgcgcg ccgttgaact ggccggtctt ctgttcgagc gtcgcgcgga tctgctgata
     6721 ggagcgaacg tcttcgcgcg aaggcggagc gatctggacg aaaagcagct cgttgaccat
     6781 gtcgtcgtgg ctggagaaca gccgttcgag cgcgtcgatc cgttcgggaa gccccttgct
     6841 gtaatcgagc cggtcgacgc cgatcgcgat cgtgcgtttg cgcgcgctgc cgccgagcct
     6901 ttgctgcgac cgcttggcga tctcggtttc gccgagcgcc atgaattcgg catggtcgat
     6961 accgatggga aaggcgcgcg cgatgctgct gcggttgccg atgcggaccg tgccgctatc
     7021 ctcgtcgacc tcggcgccat gctccttgcg gcaataatga aggaagcttt cgagccattc
     7081 gaccgactgg aaaccgatga cgtcatagtg gagcatcgcc gcgacgagcc gctcgtgatg
     7141 cgggagcgag acgagcagac gggtcggcgg ccaggggata tgcaggaaca ggccgatgcg
     7201 gtttcgtacc ccctgcgcgc gcagccgttc gccgagcggc aggaagtgat agtcctgcac
     7261 ccagacgacg tcctcctcct cgatcagcgg atagaggctg ttcgcgaaat gctcgttgac
     7321 gcgttcataa ccgcggccga actcattctc atattcggca aggtcgatgc ggtagtgaaa
     7381 gagcggccat aaggtgcggt tggcatagcc gttgtaatat tcgtcgacgt cgtggggttc
     7441 gaggtcgacc gtcgcggtgg tgaaaccgtc ggcgcgttcc atcgtgagct ggccggtgaa
     7501 ttgttcgacc tcgttgcccg accagccgaa ccagataccg ccgcgcttgt gcagcgccgc
     7561 gctcatcgcg accgcaagtc cgccctgcgc acccgccgcg ccgcgcccct tcgcgaccga
     7621 cacgcggttc gagacgacga tcagccggct catcgcaccg tgtcccagcc gcgcgacagc
     7681 agccccgcgc aatggatgat gccgacgagc gaataagtct gcgggaaatt gccccaaagc
     7741 tcgccgttct cgaaatcgag atcttcggac aggaggcccg atggcgtgcg gtgcgcgagc
     7801 atcttttcga acagctcgcg cgcctcctcg ctccgaccgc acaggtgcag cgcctcgatc
     7861 agccagaagg tgcagaagtt gaaggcggtc tcgggcagac cgaaatcatc ctcgctgtcg
     7921 tatcgcagca tatgctcgcc gcggcgcagc gccttttcga tcgcggcgaa cgtcgcctgg
     7981 aaacgcggat tgtcggcgcg gatataacgc agctcgacca tctgcagcag gctggcgtcg
     8041 agatgcgcgt ggccgaaggc ggcgccataa tggcccgccg cctcgtccca cgcctcgcgt
     8101 tcgatcgttt cgcggatctt cgccgcgcgg tcgttccaat aggcttcgcg cttgggcttg
     8161 ccgatccgcg ccgcgacgtt gccgagccgg tcgcacgccg cccagctcat caccgccgaa
     8221 taggtgtgga cgagctgttt ggtgcggaat tcccacaggc ccgcatccgg ctggtcgtgc
     8281 atcgcccacg cgacttcgcc gacgctttcg aggctttcga aatcgcgctc gtcggccata
     8341 cggaacagcc gctggtcgaa aaagccctgc accgtcggca gcacgatctg gccataggcg
     8401 tcgtgctgca cctgctgata ggcggcgttg ccgatgcgca ccggtcccat gccgcgataa
     8461 ccgccaagat cttcggcgac accctcgtcg agctcggcga cgcccatcac cgaatagagc
     8521 ggctggacct gcccgcccgc ggcgcgatcg acaatattgc ggaggtaagc gagatatttt
     8581 tcgagcacgt cgagcgcgcc gagccggttg agcgcctgca ccgtgtaata ggcatcgcga
     8641 atccagcaat agcgatagtc ccaattgcgc tcgctgtgcg gcgcctccgg gatcgaggtc
     8701 gtcagcgcgg cgacgatcgc gccggtttcc tcatgctgac aaagcttgag ggtgatcgcg
     8761 gcgcggatca ccgcgtcctg ccagtccatc ggcgtcgcga gcccgcgcac ccagctacgc
     8821 caatattgac ccgtcgcttc ctccattccg cgcacggatt cgcggacatt gcccgtaaag
     8881 ggctcgtcgg ggccgaggaa gaaatggagg tcggactcga cgcggaaagc gagctcttcc
     8941 atgacatagc cgaccggcgc atcggtggtg aggcggagcg gctggttgcc gacaaggtag
     9001 cggatatgat tggtgccgcg cgtcgtcggc gcgcgttcgg caccatggtc gcgcatcggc
     9061 gccagcttca tccggatgcg cgggctgccg gcgacggggc gaacgatgcg cgcataggcg
     9121 acgggccggt acatgcgtcc cgagcgttcg gaacgcgggc aaaagtcgat gacatcgacg
     9181 gcgctgccgt cctcagcctc aagacgtgtt acgaggatcg gcgtgttgcg gctgtagcgc
     9241 ggggtagcgc gcacctgtcc gtcgagcgca aagcgccaca cgccctcgtc ctggcgatcg
     9301 tcgttcatca gggcgcaaaa gaccggatcg ccgtcgacgc gcgggacgca tccccacacg
     9361 agccccgccg cgctgtcgat gagcgcgctg acctgacaat tgccgatcgg ccagagcgac
     9421 aaattgctca caggcccagc caacgataga cctccgccgt gctcgaaagc cggtaatggg
     9481 cagcggtcgg gcgcggtgcg ccgacaagaa tgccgtgtcc gcccgcggcg gcggcggcgg
     9541 caaagccgtc ctcgtcggtg acgtcgtcgc cgatgaaaac cggtgtggcg ccgacatagg
     9601 gcggttcgtg cataagccgt gccacggccc gtcccttgtt ggcttgcact ggccccagtt
     9661 cgacaaccat cttgcctcgc cgcacggcca ggccttcgcg ttccgcaatg tcgtccatgg
     9721 ccgtaaagac ggctgcctcg gcgtgtggtt cctgccggta atgaattgcg atgccgtgcg
     9781 gcttggtttc gacaagcagt ccgggccact gcgccgcgag cgacgcgatc gccatcttcg
     9841 tgtcgctgga tagcgtggcg gcgggagcag cggcatcgcc atgttcggcg ccgtgcgaac
     9901 ccacaatcct gatcgcgccg cgccccagat gcccgcctat gtccgaaagc gaccgcccgg
     9961 tgatcagcgc gagacggcca tccacgcgcg ccgccaagac ttcgagccgc cgcacgagat
    10021 cgtcgggaac caaaatagcg tccggcgttt ccgcgatgtc aacgagcgtg ccgtcgaaat
    10081 ccagaaagag tgccaccggc tgctgtgccg cccattccgt aagcggtggc ggaagcggga
    10141 gattgtccct cttgtgcatc gcagcaaggc tagaggtcgg ggatgcaggg gtcaaccatc
    10201 gaccaagagg ccggagggtg gctgctattt gcctcggcaa tcggcacaga gaccgatgac
    10261 ttccacgatg acgcggttcg ggcggaagcc tgccgctcgg cacaaccggt cgatttcggc
    10321 atcatatgcc gcgtcaagcg tgcgggtgcg accgcaaccg cgacaaattg tcatcgctcc
    10381 gggctggccg tcggcttcga aataggcatt gagcgactcg acccggcgga cgcgcccgcc
    10441 caaccggtcg aggatgcgat agacctgatt gggcgccagc ggcacgccga gcgccaggct
    10501 ttggcgcgcg atcctgtagg ctccgagcgg ctgcggactc gcgctcacga ttgccatgac
    10561 gatttcctca agctccgcgg gcgtccgctt ggcattttcg cgccgcgtat ctttgcgacc
    10621 tgcaccggga ccgggcgggc ggggcggctc gtcatgctca ggcaagggtc cggcgtagcc
    10681 gccagttgat gagatgcccg ccgatcagga acaggctgcc gatcgccgtt atccccgccg
    10741 atgccggctc cccccaggcg gggaacagcg cggcgacgat acaggcgacc cccgccgagc
    10801 caagctgtgc cggaagaagg gcgcggtggc ggcggtagcc gagaatgaac gcgagggcgg
    10861 ccgcggggac gaccagcgcg gccgccacaa tgtggaaaag ctccgattcg aaaaaggtcc
    10921 cggccagcat cggcaaggcg agcaggatca gcggcaatgc cagacagtgt ccgaggcaaa
    10981 gaagcgaggc ggacaccgcc gttccttcga cgaggttcgt cgtcatttgg cgccgcgaag
    11041 ggcgcgaggg gagagcgcac atggaagaaa agccttaggg gttgtgatat gagataacgt
    11101 atcatatacg gccggcgtcc agataagaaa ggggtttttg tgaagtttcg agttctcctc
    11161 ctgtccgccg cggttccggc ggtcgttccg cacatcgcct atgcgggcga atcggtgccg
    11221 gcggccaaac cggctgcagg gcccgacgcc ggcttcatgc aggccggcag caccgccgat
    11281 cccgagatcg tcgtctccgc caatgtcctc ggcctccgcg ccgacgagac cgcaacgccg
    11341 gtgatcacgc tgaccggcga ggaactgatt catcgccgtc aggcgacgct cggcgacacg
    11401 ctcgccgggc agccgggcat aaatttcgac aatttcggcg gcggtgcaag ccgtccggtg
    11461 atccgcggcc agaccgcacc gcgtgttcag gcactctccg acggcgccag cgtccacgac
    11521 gcttcggcga tctcgcccga ccatgcggtg acgacagaac ctctgctgct gcgcggaatc
    11581 gaagtgctgc gcggcccttc ggccttgctc tacggtggca gcgcgatcgg cggcgcgatc
    11641 aatcttcttg acgagaaggt gccgacctcg atccccgacg gcggcatcgc gggcacggtc
    11701 gaggggcgct tcggcaccgc ggacgacgaa cggtcgctcg tgggcgggat caccatcggc
    11761 gggcaggggc tcgcgctgcg cgtcgagggc gtccatcgct cgtcgaacga ctatcgcgtg
    11821 ccgaagggcg ttggggagcg gcatgtcgac gggtcgtaca acgacacttc gaccttcacc
    11881 gtcggcggct catgggtcgg cagcggcggc tatctgggcg ttgcctacac ccgccagcgc
    11941 agcgaatatg gcctgcccgg gcacagccac gactatgaaa gctgccatcc gcacggcagc
    12001 agcctccatt gcggcggcca tgggcacgac gacgaggatc atgatcacga ccatgatcac
    12061 gaccacgagc atgaggaggt gccgttcgtc aaattgcgca gcgagcggtt cgacatccgc
    12121 agcgaatatg aggatccgct tcccgggttc gaaaaggtgc gcttccgcct gtcgttcacc
    12181 gattatgagc atgacgagat cgagcataac gaggtcgcga cgaccttcag caacaaggcg
    12241 cacgagcttc gcttcgaact ggcgcacaag ccgatcggcc ccctgcgcgg ggcgttcggg
    12301 gtccagcatt cggaaagcca gttcagcgcg gtcggcgagg aagcctttct gcccgaaagc
    12361 gagacgcgca ataccgcgct cttcctgatg gaaacgctcg atgccggtcc ggtccggttc
    12421 gaactcgcgg cgcggcagga gtggcagcgg atcgagacga cgctgaaccg cgaggtcagc
    12481 cacaggccct tctcgatctc gggcgccgcg atctgggaca tcggcggcga ttattcgctc
    12541 gcgctgtcgc tcgcgcgctc gcagcgcgcg ccgaatgtgc aggagcttta tgcgcgcggc
    12601 attcatcttg ccacgaacac ctatgaactc ggcaccgcga cgctcggcaa ggaaaccggc
    12661 aagtcgatcg acctgacctt ccgcaagacg aaaggcgcga cgacctttac gatcggcgcc
    12721 tatcatcagg atttcgacga ctatatcttc gcgaacacgc tcgaccagtt cgaggatttc
    12781 cggctcatcc gctacaccgc cgccgacgcg accttcaccg gggtcgacgg cgaggtgcgg
    12841 catgatttcg gcaccgtcgg cgtgtcgctg ttcggcgatt atgtgcgggc caagttcaag
    12901 ggcgatctcg gcaacctgcc gcgcattcct gccggccgtc tcggcgcgcg cgccgacggg
    12961 cggtgggggg cgctctcggg cgacgtcgaa tattatcatg tcttcgaaca ggacaggatc
    13021 gcggcgtttg aaacgcggac gtcaggctat gacatgctca acgcgacgct tgcctacaag
    13081 ctcgcgctcg ggccgaaggc tgacgccgaa ttgttcgtgc gcgcgacgaa tttgaccaac
    13141 gaactcgcat ataatcacgc gtccttcatc aagaatgcgt cgccgctgcg cgggcggaat
    13201 ttcgtcttcg ggctgcgaac gagcttctag ggccaaaggg ggcggccgga aggaggggca
    13261 catggtcccg cgcccctcct tccgccgatt ttgtcaggcg ggctcggccg ccaggacata
    13321 gtcgaagccg tggcgaagcc cgtcctcgcg cgcgacatgc gcgagggctt cggcgcgcag
    13381 cgcctcgccg cgcatctcaa gggcgtccgc gaggccgcgg tcggcgatcc ttgccagccg
    13441 cggcgcgcac atgtcgatct ggctgttgac gaagcgctcc ttgtagcggt tcgcgaaccg
    13501 cttggcggcg atgacgcgat accccgatgc ttcgagatga tcgaccgtcc attgcgccgg
    13561 atattcgcga tatggctgtt cgcccgcgtg gagcaggcac gcgtcgcgga agcgtccgat
    13621 ctcccagatc agccgccccg cttcgctatc gggctcgccg ctcacatagg gttccagtcc
    13681 gacgacatag agccgccgcc cgacgtgcgg acgcagccgc gcgaacaggc gatgctggaa
    13741 atagggcgcg aaaccgtcga tcgcgccgag aagatagtcc gccagcaccg tgtcatatcg
    13801 ctcacccgcc aacagcgccg ggtcggccca gttgccgacg atgatgcggt cggcggcgcg
    13861 gcgcttcgcc gcgaccgcat cggcgacctg cgcggcatgg gcttcggcgc cgctgaccgc
    13921 ggtccagcat tcggtggcga gcgaaccgat ccagccgatc gagtgcgttc ccgtcccggc
    13981 gtcgagaagt ttgccccacg cctgcccgtc ctgaagggct tcgatttcct gaaaaagccc
    14041 ggaagccgac cgtcgctctc taccatcaaa tatcattgac atgatataga gtatcattgc
    14101 ggccttatca aacccgaacc cgcggaatgg caaattcagc ggcgcatcgg ccttgcgcga
    14161 gcggcgatca acgccgcctg gtgccgtagt cgatccggat tcggacccaa ctgcccacca
    14221 tcggccgccc gccaagccgc gggggccgga cctggaactg ccacgctgcg gcgagcaccg
    14281 cgcgattgat ctgcgatcca tagggatatt cgtcgagccc gacgcaatcc tcgacacggt
    14341 aatcgggcgc cgtgcggcag gcgatcaatc cccagccggg cccgctggcg gtcgacagat
    14401 agccgcgcag ttcaccatcg cttggctcgc ggtaccaggc ggccgcatat agcgcctcgc
    14461 cattgggtgc cgttcccacc cgttcggagt cgcgtgagga gggtgatcca ccggtattgg
    14521 gcggcccgta aacctgcccg ccctgcggac gtgcggcctt cggggggctc gccggcaacg
    14581 gccgctggac gggcattgct atgggcggcg cgaccttcgg ttcggggact tgcgggggca
    14641 gcggttccaa cggctgcggt tcgtcgggtt ctggctgcgg cgaggtggtt cgttcctcgg
    14701 cccgctcgcg ttcggggctc gccggctcgg gcgcatcttt cgcaatctcg gtggcctcaa
    14761 ggctcaccac gctgatcgac tcctcctcag cgtcaggctg cgccccgggc ccccaggaga
    14821 ggagcatgag cagcatcagt ccggcgatcg ccacggccag gccgatggca agcgagcggc
    14881 gccccacccc gatgttgagc tgtgcgacgt agggcgtggc cggagcgggg ttcagtcgaa
    14941 gcaaggtgtt cagcgtagcc ctgcacaagt ctggaaagat atggctcccc gggtaggatt
    15001 cgaacctacg accgatcgat taacagtcga ttgctctacc gctgagctac cgaggaacac
    15061 gccgccgatg cggacaaggc ggcccctttg ccgccaaccg atataattat caagagggtt
    15121 ttggcggctt tgtgattctc agacgataaa ttgttccatc gcgatgcgtt cgtcgaggcc
    15181 ctggtcggga tcgaacagca aagtcagcgg ccggctgcgg tcggtcgtga cgagcacggt
    15241 cttcacatcg cgaatctcgc gctggtcggc gaccgcgctg accgggcgtt ttgcggcctc
    15301 gcggacgttg aagcggatgc tcgtcgattc ggggacgagc gcgccgcgcc agcgtcgcgg
    15361 gcggaaggga ctgatcggcg tgagcgcgag catcgccgaa tcgagcggca ggatcgggcc
    15421 gttggcgctg aggttatacg cggtcgatcc ggcgggggtc gagacgagca cgccgtcgca
    15481 cgccagttcc tcgagcatcg tcttttcgtt gatcatgacc tcgagtttcg cggcctggcg
    15541 cgtttcgcgg agcagcgaga tttcgttgat cgcgggcaag atgtgccgct cgccgctgat
    15601 cgtcgtgatg tcgcccgaca gcggatggat cagataggga cgcgcggcgg cgatgcggtc
    15661 gagcagcccc tcgacgcgaa actcgttcat cagaaagccg atcgtgccgc ggttcatccc
    15721 gaagaccggc aggctgcggc gctggtcgag caattgatgc agcatgtgca gcagaaagcc
    15781 gtccccgccg agcgcgatca agaggtcggc ctcggtcaga tcgacgaagt cgtagagcgg
    15841 gcgaagttcg gcttccgctt ccatggccgc gggcgcattc gatgcgacaa gcgcgatctt
    15901 gcgatgcata ttggtccggt tcccccgtgc cttcgaatat cggaacggcc tgtcagccgc
    15961 ccgttgcact catgtgacgc gcgaccgccg gtttggactc tcgttcgata tgaaagtcat
    16021 gcgcgcgggg tttgccagcc aacgcggcat caatcaagcc gtcgacatcg ccgccgtcgc
    16081 gaagcgcagc gcgcagatcg acgtgatcgt cctggccgag gcacatatag atgcggccct
    16141 cgacggtcag gcgaatgcgg ttgcacgtcg cgcagaaatt gtcgctgagc ggcgtgataa
    16201 ggccgagcgt caccaggctg ccctccaccg cgaaatagcg cgcgggaccg ccggtccgtt
    16261 tgtcgaccgg atagatggcg cgcgtttcgc gcagcggcgc gatgaaatgg tggatcggga
    16321 tatagtgatc gctacggtcg ccttcgacct cgccgagcgg catcgtctcg atcaaggtga
    16381 ggtcgcagcc ggtcgccgcg caatagtcga gcatcgggag caattgatcc tcgttgaggc
    16441 ccgcgagcgc gaccatgttg atcttgatcg cgaggtccgc tgcgcgcgca gcatcgattc
    16501 cgcgcagcgc gacatcgaga tcgccgacgc gcgtgatatg ccggaatgcc gccggatcga
    16561 gcgtgtcgag gctgacgttg atccggcgca cccccgccgc cgccagcatc ggcgcatgtt
    16621 cggcgagccg catcgcattg gtggtcagcg tcagttcgtc gaggccgtgg ccgatcagcg
    16681 cgccgaggcg catcgcgagg gtatcgatgc cgcgccgcac gagcggttcg ccgcccgtca
    16741 gccggatgcg gcggacgccg cgcgcgacga aacgggttgc cagttcggcc atttcctcga
    16801 tgctgagcac cgccgacttg ggcagaaagg tcatgtcttc ggccatgcaa taacggcagc
    16861 gcaggtcgca gcgatcggtg accgacaggc gcaagtaact gatccgccgg ccaactccgt
    16921 cgatcagggg cgcccgcgac gcggcgcgat gagataatgg gacagccacg gacatattgt
    16981 ggtgcaaaag gcacgagctt ggcaaggaaa gcctgctagg aaagcgcgat gaatcgcttg
    17041 tccgaatccc tttccgcctg tgttcgcaaa ctcgaagccc tgcatgcccg gcatgatgcc
    17101 gacgcgggcg tcatcttcgt ccaggtcgac cagatggcgc gcatcaacaa cagcgccggg
    17161 acggcgacgg gcgattcggt actcgacgaa atcgggcggc ggctcgaaaa tttcgcgagc
    17221 gacgaattcg gtcagggatc gtgcgtcgac cggctcgacg gcccgcgctt cctgatcgtg
    17281 cccgcaacgc cgctgtcgct cggggcgctc cgcgcgcagg aacgcgcgct gcatgtcgca
    17341 ctcgccgagc cgatcgtcgg cgatccgaat ggccgcctgt cgatccgcat cgccgccgcg
    17401 ctgatcacgc gcgacgaatc ggttgccgaa caattgcgcg cggccggcga ccaactcgcg
    17461 cggccggctt cggcgcgcga cggggtgatg gtccacgccg ccctgtcgcg caacgaggtc
    17521 gtgatccatt accagccgca atatgacg
//
LOCUS       DATMSU010000076         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_96398, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000076 DATMSU010000000
VERSION     DATMSU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96398"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..960
                     /locus_tag="VN034_02075"
     CDS             184..960
                     /locus_tag="VN034_02075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWV59412.1"
                     /translation="MTSLSPASPRPFLALSSFRFRLWPIVLAAVLMQVILEFGRIPAR
                     EIYRAGAPLWDGYVSVFLLLAIAFQALLGLAAILIMRRWLPAADPHLRWPPGASYVGI
                     AFLIGVGMGLVMLVADYWPALAAQTAPDMSYSKAPLDSAGYLLGMITTGLAEETIFRG
                     LLVGMLVVLVPGRLRIGSLDLPVAAYLVALMFGLAHWKSFTVDPFYQAMAQQIYAFAW
                     GLAYVWLMERSRSLLAPIVAHGMGNFTEVAIVIALNALWA"
     gene            complement(1007..1501)
                     /locus_tag="VN034_02080"
     CDS             complement(1007..1501)
                     /locus_tag="VN034_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59413.1"
                     /translation="MILGLTIPQFTALHVAISLIGIGAGLIALPAFVRGRLLRRTTHI
                     FLWATLLTSLTGFLFPIIAFTPALGTGILSTLILAFAFAAFYGRKLAGRAATVYAVSA
                     TAALYLNLFVLVVQSFLKVPALNALAPSGTEAPFAAAQGALLLAAILFGYAAVRASRR
                     PIAV"
     gene            complement(1624..2451)
                     /locus_tag="VN034_02085"
     CDS             complement(1624..2451)
                     /locus_tag="VN034_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006958336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59414.1"
                     /translation="MTTIATKDGTNIFFKDWGPKDGQPIIFSHGWPLSADAWDAQMVF
                     FANQGFRTIAHDRRSHGRSDQVWDNNNMDQYADDLAELIEQLDLKNIILVGHSTGGGE
                     VTRYIGRHGTSRVAKVALIGAVPPLMLKTEANPGGLPIDVFDGIRKGTFDNRPQFFKD
                     LTLPFYGYNRDGAVVSEAVRDDFVRQGMNGGLKGLLDSIRAFSESDFNEDLKKFDKPT
                     LVLHGDDDQIVPIGAAALSTVKIVKHAVLKIYEGADHGLTVTHQDRFNADLLDFINS"
     gene            2580..>2898
                     /locus_tag="VN034_02090"
     CDS             2580..>2898
                     /locus_tag="VN034_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59415.1"
                     /translation="MPRLTVPDAANVPLVEVGMMLYPDCQIGMVHGITDLFDVAGRFA
                     TDHGRRPIRASHWRLQEGGGFARCFDSHPDEPAGNSPAVLIAPGSLHKLLEADEVAPY
                     ARWL"
BASE COUNT          461 a          855 c         1021 g          561 t
ORIGIN      
        1 cgcgcgcctg gccacgacga ccaccccgct gcgactaacg aacaagttcg taagtctcgc
       61 tacccctcct ctgaagagga ggggctgacg catatttctc aatgtccgct aatggccgaa
      121 acccgccttc aagcatcaca ttcgttcaag aattgcggga cgctttgcgc ctaagccggt
      181 cgcatgacca gtctctctcc cgcttcgccg cgcccgtttc tggcgctttc cagtttccgc
      241 ttccgcctgt ggccgatcgt gctggcggcg gtgctaatgc aggtcatcct ggaattcggc
      301 cgtattcccg cgcgcgaaat ctatcgtgcg ggcgcgccgc tctgggatgg ctatgtgtcg
      361 gttttcctgc tcttggcgat cgcgtttcag gcgctgctcg gcctcgccgc gatccttatc
      421 atgcgccgat ggctgcccgc cgccgacccg catctgcgct ggccgccggg ggcgagttat
      481 gtcgggatcg cctttttgat cggggtcggc atggggctgg tgatgctcgt ggccgattat
      541 tggcccgcgc tcgccgcgca gaccgcgccg gacatgagct attcgaaggc gccgctcgat
      601 tccgccggct atctgctcgg gatgatcacg acggggctcg ccgaagagac gatctttcgc
      661 ggcctgctcg tcgggatgct cgtcgtgctg gtgcccggac ggctgcggat cggatcgctc
      721 gacctgcccg tcgccgccta tctcgtcgcg ctgatgttcg ggctggcgca ttggaaatcc
      781 ttcaccgtcg atccctttta ccaggcgatg gcgcagcaga tttacgcctt tgcgtggggg
      841 ctcgcctatg tctggctgat ggagcggtcg cggagcttgc tcgcgccgat cgtcgcgcac
      901 gggatgggga attttaccga agtagcgatc gtgatcgcgc tcaacgcgct gtgggcgtga
      961 cacgacaagg gcggccatcg cgccgccctc gatcctttgg ggccgatcag actgcgatcg
     1021 ggcgccggct ggccctgacc gcggcatatc cgaagaggat cgcagcgagc aggagcgcgc
     1081 cctgtgccgc cgcgaacgga gcttcggtgc cgctcggggc gagcgcgttg agcgcgggga
     1141 ccttgaggaa ggactgcacg accagcacga agaggttgag gtagagcgcg gcggtggcgc
     1201 tgaccgcata gacggtggcg gcgcggccgg cgagcttgcg gccgtaaaag gcagcgaagg
     1261 cgaaggcgag gatcagcgtc gagaggattc ccgtgccgag cgccggggtg aaggcgatga
     1321 tcgggaagag gaaaccggtg aggctggtga gcaaggtcgc ccagaggaag atgtgggtcg
     1381 tgcggcggag gaggcgcccg cgcacaaagg cgggaagggc gatgaggccc gcaccgatgc
     1441 cgatcaggct gatcgcgaca tggagtgcgg tgaactgcgg gatggtgagg ccgaggatca
     1501 tggtcttact ccttgttttc tctccccgtt cgccctgagt ttgtcgaaag gccgctcttc
     1561 cctgcgaggc agggagtgaa ggacggtgct tcgacaagct cagcacaaac ggaggaggga
     1621 aggtcagctg ttgatgaaat cgagcaggtc ggcgttgaag cgatcctgat gggtgacggt
     1681 caggccgtgg tcggcgcctt cgtagatttt cagcaccgca tgcttgacga tcttgaccgt
     1741 cgacagcgcc gcggcgccga tcggcacgat ctggtcgtcg tcgccgtgca ggacgagcgt
     1801 cggcttgtcg aacttcttga ggtcctcgtt gaagtcgctt tccgaaaagg cgcggatgct
     1861 gtcgagcagg cccttgaggc cgccgttcat gccctgccgc acgaagtcgt cgcgcaccgc
     1921 ttcggagacg accgcgccgt cgcggttgta gccgtagaag ggcagggtca ggtccttgaa
     1981 gaactgcggg cggttgtcga aggtgccctt gcggatgccg tcgaagacgt cgatcggcag
     2041 gccgcccgga ttggcttcag tcttgagcat cagcgggggg acggcgccga tcagcgcgac
     2101 cttggcgacg cggctggtgc cgtgacggcc gatgtagcgg gtgacttcgc cgccgccggt
     2161 cgagtggccg acgaggatga tatttttgag gtcgagctgt tcgatcagtt cggcgaggtc
     2221 gtcggcgtac tggtccatgt tgttattgtc ccagacctgg tccgaacggc cgtggctgcg
     2281 gcggtcgtgg gcgatggtgc ggaagccctg attggcgaag aagaccatct gggcgtccca
     2341 ggcgtcggcc gacagcggcc agccgtgcga gaagatgatc ggctgtccat ccttcggacc
     2401 ccagtccttg aagaagatgt tggtgccgtc cttggtggcg atcgtggtca tgtcatgctt
     2461 cctttcgttt cgagaagggc gggatgtccg tccggtgaaa caacagatac ggcgatgcgt
     2521 gcttgatcgg gattggcgga cgcgacatta tccgggccga ttccgacaaa ggagccgcga
     2581 tgcccagact gacggtgccc gacgcagcca atgtgccgct cgtcgaggtc gggatgatgc
     2641 tttatcccga ctgccagatc gggatggtcc acggcatcac cgacctcttc gacgtcgcgg
     2701 ggcggttcgc gaccgaccat gggcgccgcc cgatccgggc cagccactgg cgcttgcagg
     2761 agggcggggg tttcgcgcgc tgtttcgaca gccatccgga cgagccggcg ggcaattcgc
     2821 ccgcggtgct gatcgcgccg gggagccttc acaagctgct cgaggccgac gaagtcgcgc
     2881 cttatgcgcg ctggctgg
//
LOCUS       DATMSU010000077         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_96432, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000077 DATMSU010000000
VERSION     DATMSU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96432"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..88
                     /locus_tag="VN034_02095"
     tRNA            14..88
                     /locus_tag="VN034_02095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Thr,seq:cgt)
     gene            complement(154..1410)
                     /locus_tag="VN034_02100"
     CDS             complement(154..1410)
                     /locus_tag="VN034_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWV59416.1"
                     /translation="MAGTDDYRATLAAARPRAMAALLRYFRDLDQAEDAFQEACLRAL
                     SAWSKNGRPRDATGWLILVGRNAQVDALRRHQKLTNGVDETIAHAHSGGEPDLLDRLD
                     ESHFRDDILRLLFTCCHPDLPPAQQIAVALRVISGLSVPQISRAFVVSEAAMEQRITR
                     AKARIAKAELPYEAPGAIERVERLAAVSKMLYLLFNEGYTAADPASARAGLCAEAIRL
                     VRLLLEMFPAEPELMGLLALMLLQHSRAAARFDAAGSPISLDDQDRLLWNRDAIAEGS
                     ALIDKAMRHRAPGPYQIQAAIAALHARAETPEDTDWPQIDLLYQALERHAPSPVTTLN
                     HAIAVARIHGAAAALEMIEPLSGALENYFYFHGVRGIFLKEVGSRQEARRALLRAMSL
                     ASTPAEAVHIRREIEHLEQRGGPVPG"
     gene            complement(1397..1798)
                     /locus_tag="VN034_02105"
     CDS             complement(1397..1798)
                     /locus_tag="VN034_02105"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWV59417.1"
                     /translation="MLYAVLCYHDEAITGAWAPAEESAVADRLIAVHDKYGDRFRPVL
                     RLMPASTAMTMKSGMAVMTDGPFTETKEQLLGIYTIDVDGLDDAVAIVADLSAANPTA
                     HYELRPLIAYITDAAFGPVQAIEGAQRDGRY"
     gene            complement(1856..2284)
                     /locus_tag="VN034_02110"
     CDS             complement(1856..2284)
                     /locus_tag="VN034_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59418.1"
                     /translation="MTKMIFINLPVADLAASTAFYMALGFTKNDQFSDETASSMRWSD
                     QITFQLLTREYFQTFTSKTVGNAHGDCQLLIALSADGREEADRLAELAGAHGGKTDPR
                     APMDLGFMYNRAFEDPDGYVVELVWMNPDVAMDDAAAPAA"
     gene            complement(2543..5797)
                     /locus_tag="VN034_02115"
     CDS             complement(2543..5797)
                     /locus_tag="VN034_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59419.1"
                     /translation="MQIMSSLNSDSQRQAKAALRGYIYQIVRSIDIWLDLEDREELVL
                     EGAEDFDRISDEQAVVGQVRETRGSGNITLRTAGVIDAINDFWTHRSRNPGFNIRYRY
                     LTTSGIGMERGRPFGPARGGLHIWSRIRAAPLTERSVADAEIVAGFLRDQAAIRDELK
                     DYLETAAPSEIIEGLIEPIEWSVGQGDADALIRKIKDKLVLHGERNNVASANAEKVFD
                     ALQTAAFEAASTRDAPPLTRAAFLRIFDNRILVQVPPTPSGTAARHVPDENASGEAKE
                     QLQRHVIASNQHFSIFGVAEPLSMDRGWVELPIFVPLADESADPATVDEILERYRNGR
                     NSTSHPGISGIANISIGRFYRHCVVIGGPGLGKSMLTRKLALTYARDGFPVIQVDLKA
                     LALAMRQGGTFLGNILTLGLGDASVSPDMIAPAFLASGVLLCDGLDEAGDAQDLIARH
                     LAAFAETHPDCRVIVTIRPIGYETPHLAGWRHYEILGLDSDSAGDRVETLLTAIHPEA
                     PAERLAIRRFVEGQLERNRDSGPMTHSPLLLGLCVLLGIHEEEFGSSRNELYERVFDR
                     IEQPPNVRIDARPSGSLATRCVDVLGWVLTENPNAKLKQIRARCADILAGELGKSRLA
                     AEMDFEQCIAFWQQTGMVERVRHAGNDMLLFVHKTFGEFAGARYMAGLPDDAARDALI
                     LAQSQRASSMELLRFAGGLGLAESVVGALLTPTDRGASHAMKILYCMEILADGDGALS
                     AGLVDEILDAAFALARSSPVAAGSICPAMLHAVKYYPGEIAARARPLRDDPSNWLRLV
                     AWACLATAGARHYDLDVLVSAFLELPDLGMHPEDFGPSPGSADRDEIQDLAVAFASAA
                     LGEILKQLPTDKSDALLATLLPRISAGDTPPSVEFMRRAMASLRSAGRGDDAERLFAR
                     WHVSSLEQQEEYASDFERVLKIVMDLLVDGAEEDSVEGKEDGVHDLYLHLGAYLEATR
                     FVVTSNNGWEQWQQCHDREAASAVVRALLSLCVVDPAALRTDIRTLRAEVEARPKDAL
                     SRQWRLWERIDIPEPDYSGARMLDLSPALIEKAKQHPSTWISEAAARLYDGRKP"
BASE COUNT         1227 a         1910 c         1865 g         1185 t
ORIGIN      
        1 ggcgcccctc gttgccgctt tagctcagtt ggtagagcac atcattcgta atgatggggt
       61 cacaggttcg agtcctgaag cggcaccaca agccccataa atcaataact tagggctgac
      121 accccctact tcccgaagtt cgattcgccc aacctaaccc ggcaccgggc caccccgctg
      181 ctcgagatgc tcgatctcgc ggcggatatg gacggcttcg gcaggtgtcg atgcgagtga
      241 cattgccctc agcaacgctc gccgtgcctc ctgccgactt cccacttcct tgagaaaaat
      301 accgcgcacg ccgtggaaat agaaataatt ctccagcgcg ccggatagcg gctcaatcat
      361 ctcgagcgcc gcggcggcac cgtggatgcg ggcgaccgca atagcatggt tgagcgtggt
      421 aaccggcgac ggggcgtgcc gctcaagagc ctgatacaaa agatcgatct gaggccaatc
      481 cgtatcttcc ggggtttccg cgcgtgcgtg gagggccgcg attgcggcct ggatctgata
      541 aggcccgggc gcgcgatgtc gcatcgcctt gtcgatgagc gcggagccct cggcgatggc
      601 gtcgcggttc caaagcaggc ggtcttggtc atcaagcgaa attggcgaac cggccgcatc
      661 gaaacgggca gccgcgcgcg aatgctgcaa cagcatcaga gccagcagcc ccatcagctc
      721 cggttcggca ggaaacatct ccaatagcag ccgaacgagc cggatggctt cggcacagag
      781 gcccgcgcgg gcgctggccg ggtcggctgc cgtgtagccc tcgttgaaca gcagatagag
      841 catcttgctt acggcagcga ggcgttcgac acgttcgatt gctcctggcg cctcataagg
      901 caattccgcc ttcgcaatcc tcgccttcgc ccgcgtgatc cgctgctcca tcgccgcctc
      961 gctcaccacg aacgcgcgcg agatttgcgg gaccgacagt ccggaaataa cgcgcaaagc
     1021 aaccgcgatc tgctgcgcgg gcggaagatc gggatgacag caggtgaaga ggaggcgaag
     1081 aatatcgtcg cgaaaatgac tttcgtccag ccggtcaagc aggtcgggtt cgccgccgct
     1141 atgggcgtgg gcgatggttt cgtcgacccc attggtcagc ttttgatgcc tccgaagggc
     1201 atcgacctgc gcgttgcggc cgaccaggat cagccatccc gtcgcatcgc gtgggcggcc
     1261 atttttagac caggcggaca acgcccgtaa gcacgcttcc tgaaaagcgt cctcggcttg
     1321 gtcgaggtcc cgaaaatagc gcagcagcgc cgccatcgcg cgcgggcgcg ccgcagcgag
     1381 cgtcgcgcga tagtcgtcag taccggccat cgcgctgcgc gccttcgatg gcttgcaccg
     1441 ggccgaacgc cgcgtcggtg atgtaggcga tcaacggacg cagctcgtag tgggccgtgg
     1501 gattggcggc actgagatcc gcgacgatcg ccaccgcgtc gtcgagacca tcgacgtcga
     1561 tcgtatagat accgagcagc tgctccttcg tctcggtgaa ggggccgtcg gtcatgaccg
     1621 ccatgccgct tttcatggtc attgccgtac tggcgggcat caaccggaga accggacgaa
     1681 acctgtcgcc atatttatca tggaccgcaa tcagccgatc ggcgacagcc gattcttcgg
     1741 cgggcgccca cgctcccgtg atcgcctcgt cgtggtagca taaaacggca tagagcatcg
     1801 tgcgtgtccc atctgatcgg cgacggccgg ccggagccgg ccgtctcgcc tgtcattaag
     1861 cagcgggggc ggccgcgtca tccatggcca catccggatt catccacacc agctcgacga
     1921 catagccgtc gggatcctcg aacgcacgat tgtacatgaa ccccagatcc atcggcgcgc
     1981 gcggatcggt cttgccgcca tgcgcgcccg ccagctcggc gagacggtcg gcctcttccc
     2041 gtccgtcggc cgacagcgcg atgaggagct ggcagtcgcc gtgcgcattg ccgaccgtct
     2101 tcgacgtaaa ggtctggaaa tattcgcgcg tgagaagctg gaaggtgatc tgatccgacc
     2161 accgcatcga cgaggccgtt tcgtcgctga actggtcgtt cttggtgaaa cccagcgcca
     2221 tgtagaaggc cgtggacgcc gcgagatcgg caacaggcag gttgatgaaa atcatcttgg
     2281 tcattcgagt tctcctttcg gcgaagcgag ttgcttcggc gatacaagga cgcaggccga
     2341 tggcccgccc cgacaccgcg gcgaaaaaaa atccgaagcg caaaaaaggt ccgtttggcc
     2401 ccgcttgccg gtcgagggca cagagcactt cgcgattggt ccgagaagga gtggtattcc
     2461 gaagggttcc tatcggcatt ggcgatccga cagcgagcta ttaaggctgc cgtgcccgat
     2521 ggacgttccg cgccggatcg gctcaaggct tacggccatc atatagtctc gctgccgctt
     2581 cagagatcca tgtagatgga tgctgctttg ccttttcgat caaggcaggc gacaaatcga
     2641 gcatccgggc accggaatag tcgggttcgg gaatgtcgat gcgttcccac aacctccact
     2701 ggcgcgacag agcgtctttg ggccgggctt cgacttcggc gcggagcgtc cttatgtccg
     2761 tccgcaacgc ggccgggtca acaacacaga gcgaaaggag cgcccgcacg accgcgctcg
     2821 cggcttcgcg atcatggcat tgctgccact gctcccaccc attgttcgac gtcacaacaa
     2881 agcgggttgc ctcaagatat gcgccaagat gcaaataaag atcatgaacg ccgtcttctt
     2941 ttccctcgac cgaatcctct tccgcgccgt cgacgagcag atccatgaca atcttcaaca
     3001 cccgttcaaa atcgctggca tattcttcct gttgctcgag agacgagaca tgccagcggg
     3061 caaataaacg ctcggcgtca tcaccgcgtc cggccgaacg gagtgaagcc atcgcgcgtc
     3121 gcatgaattc gaccgaaggc ggagtatccc ctgcgctgat cctcggaaga agcgttgcga
     3181 gaagcgcatc gcttttgtca gtgggaagct gcttcaggat ttcgccgagc gcagccgatg
     3241 cgaaagcaac ggcaaggtcc tggatttcat cccggtcggc cgagccggga gacggtccaa
     3301 aatcttcggg atgcatgcca aggtcaggca gctccagaaa cgcggagaca agcacgtcga
     3361 gatcatagtg ccgagcgccc gccgtagcca ggcaggccca ggcgacaagg cgtagccagt
     3421 ttgacggatc atcgcgcaaa ggacgcgccc gtgcggcgat ctcgcccgga taatatttca
     3481 cggcgtggag cattgccgga cagatggaac cggccgcgac cggggaagag cgcgccagcg
     3541 caaacgcggc gtcgagaatt tcgtcgacaa gcccggccga caatgcaccg tcgccatcgg
     3601 caagtatttc catgcaatag agaatcttca tcgcatgcga agcgcccctg tccgttggcg
     3661 tcagcaaagc gccgacgacg gattcggcga ggcccagccc gcctgcaaat cggagcaact
     3721 ccatggatga cgcgcgctgc gattgcgcca ggatcaacgc gtcgcgcgct gcgtcgtcag
     3781 gaaggcccgc catatagcgc gcacccgcga attcgccgaa tgtcttgtga acgaacagga
     3841 gcatgtcgtt gccggcgtgg cgcactcgct cgaccattcc ggtctgttgc cagaaagcga
     3901 tgcactgttc gaaatccatc tccgccgcga gcctggattt gcccagctcc ccggccagta
     3961 tatcggcgca tcgtgcgcgt atctgtttca acttcgcgtt gggattctcg gtcagtaccc
     4021 agcccagaac atccacgcag cgggtcgcca gactgccgga aggtcgggcg tcgatacgga
     4081 cgttgggtgg ctgctcgatc cggtcgaaca cgcgttcata aagttcgttc cggctgctcc
     4141 cgaattcctc ttcatggata ccgagcagga cacacaggcc aaggagcagc gggctatgcg
     4201 tcatcgggcc gctgtcgcga ttgcgctcca gttgtccctc gacaaagcgc cggatggcaa
     4261 gccgttcggc aggcgcctcg ggatggatgg cggtcagcag ggtttcgacg cgatcaccgg
     4321 cggaatcgga atcgagcccc aatatctcat agtgccgcca gcccgcgagg tgcggtgtct
     4381 cataaccgat cggccgtatg gtgacgataa cgcgacaatc tggatgggtc tcggcgaatg
     4441 cggcgaggtg gcgcgcgatg agatcctgag cgtcgccggc ttcgtcgagt ccgtcgcata
     4501 gcagcacgcc agaagccagg aacgcaggcg cgatcatatc cggggagacg ctggcatcgc
     4561 cgagtccgag cgtcaggata tttcccagga acgttcctcc ctgccgcatg gccagcgcca
     4621 gcgctttcag atccacctgg atgacgggaa agccgtcgcg cgcgtaagtg agcgccagct
     4681 ttcgggtgag catgctcttg cccaggccgg gaccgccgat gaccacgcaa tgcctgtaga
     4741 agcgcccgat cgatatgttc gcgattccgg agatgccggg atgcgaagtg ctatttctgc
     4801 cgttcctgta acgctccaat atctcgtcga ccgtcgcggg atcggcgctt tcgtccgcta
     4861 ggggaacgaa gatgggcaac tcgacccatc cgcgatccat cgagagcggt tccgccacgc
     4921 cgaagatcga gaaatgctgg ttggaggcga tgacatgcct ttgcagctgc tctttcgcct
     4981 ccccgctggc attttcatcc ggcacatgcc gggcggcagt tccgctgggc gttggcggaa
     5041 cctgaaccaa tatcctgtta tcgaatatcc tgagaaacgc cgctctggtc agcggaggcg
     5101 catcgcgcgt acttgcggct tcgaacgcag cggtctgaag cgcgtcgaat accttctccg
     5161 cattggccga tgccacattg ttgcgttcgc cgtgcagaac cagcttgtcc ttgatcttcc
     5221 ggatcaaggc atcggcatcc ccctgcccga cgctccattc gatcggctcg atcagccctt
     5281 cgattatctc ggaaggcgcc gcggtttcga gataatcctt cagttcgtcg cgaatggccg
     5341 cctgatcgcg caggaagccc gcgacgatct ctgcatcggc gaccgacctc tccgtcagcg
     5401 gcgcggcccg gattcgcgac cagatatgaa gcccgccgcg cgccggaccg aaaggccgac
     5461 cccgctccat gccaatgccc gatgtggtca aatatcgata ccggatgttg aaaccggggt
     5521 tacgcgagcg gtgtgtccag aagtcgttga tggcatcgat cacgccggcg gtgcgaagcg
     5581 tgatatttcc cgaacctctg gtctcgcgaa cctgtccgac cactgcctgc tcgtccgata
     5641 tccggtcgaa gtcctccgct ccttcaagca cgagttcttc ccgatcttca agatcgagcc
     5701 agatgtcgat gctgcgtaca atctgataga tatacccgcg caaagcggct ttggcttggc
     5761 gctgtgaatc agaattcaga ctgctcatga tctgcaattt tttcgtcttg catctcgcat
     5821 gttgtgggac atttcgtcac aattcagagc ttagtcctgc gttgagaacc gccttcatcg
     5881 aggcattggc gatgccgcgc aaacgcgact aacgaattca agataattca cgattagagg
     5941 tcaagtaatc tcaggaaaaa tattgttagg agctacggct caaactgctc aagatttgta
     6001 ccggcaccga ctacgacccc gaccgccgcg gtagagttcg ccgagcctgg ccgtgtgtgg
     6061 acggccctcc gttggcaagt ggtttctgga tgtgatgcga cctgctggtt ggtgcagcca
     6121 tgtgtccggc ctgtgatgcg gccttgtcat gcgccgctgg ccatgatgcc tttcgcgcgg
     6181 atcgggt
//
LOCUS       DATMSU010000078         5530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172147,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000078 DATMSU010000000
VERSION     DATMSU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172147"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VN034_02120"
     CDS             <1..1015
                     /locus_tag="VN034_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV59420.1"
                     /translation="SHGLVGRAPAMQAVYRTIARLASNDLAVLILGESGTGKEVVARA
                     IHATGLRRGGPFVAINMAAIPRELIEAELFGHEKGAFTGAHSRNAGRFEQAAGGTLFL
                     DEIGDMPLEAQTRLLRVLQSNEYSTVGGSQALRADVRVIAATHRDMRTLVADGRFRED
                     LFYRLNVIPVTLPPLRDRRSDIAALVGHFVDAGRSSGLPHRQFAPAAMQWLERYDWPG
                     NVRELGNVVQRLAVLSRDTVVTVRDVETVLRENGEGGGAVEPAALIARAVDDWTREQM
                     AGSTNDGDIHEQLEAIVEAALFRRVLREVRGNQLEAARRLGINRNTLRKRLGQLDIDP
                     AHP"
     gene            1137..3398
                     /locus_tag="VN034_02125"
     CDS             1137..3398
                     /locus_tag="VN034_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV59421.1"
                     /translation="MDSDEEEARSGFWRFGTPEYYTLGIIVSVAIATYIFVTGDAQSE
                     RLLTPALVAAIMVVNLVPAMALIVLIGSRVARARATRSMGGGNGRLHVRLVALFSLIA
                     AAPTLLVVIFASLLFQFGVDFWFSDRSRGMFENAASLAEGYYQENQRQVGANTFAMAN
                     DLGTRLSRFSIDSPGFTNYYLQQVVVRSLNESAIIEIGRDGVARTAAAIDPDDRSAEN
                     RLSSEMVGRLDAGEDVVVVRQANRIEAAAQLPGTDRAYVYASRDTNVPGFQQSARASA
                     VLADYNGLFERSQLLQLQFNGALYLGSLLLLALAVIAAIVVADRIVRPLGTLIGATRT
                     AADGDLSVRVTPPVRDDEIAVLTRAFNRMTEQLEGQTGALVSVNEQLETRRSFIEAVL
                     SGVSSAVVSVDAGGRILLANAAAERLIGQSAEDLTGQPLDDVAPELARLLTEEEREGI
                     VQLSRPDAEPATLAAKAVAQGDGFVLSFEDITQQLLDQRRAAWSDVARRIAHEIKNPL
                     TPIQLAAERLQRRFGDKVEGDAATFRKLTDTVIRQVHDMRRMVDEFSSFARMPKPTFG
                     VEDVRDILRQAVFLFEVAKPDIAFSVKTPAEIEPLVCDRRSMSQAVTNIVKNAVEAIE
                     EKYKNSDSAATGHISAALDIGAGGEVIIRVTDDGIGLPEARDAIAEPYMTTRQGGTGL
                     GLAIVKKIVEQHYGELEFSDNPAGRGTCVTLTLHPDRLRPLAGKGGEQNMGQNETVPG
                     RIRNRQDREDHGA"
     gene            3388..4767
                     /locus_tag="VN034_02130"
     CDS             3388..4767
                     /locus_tag="VN034_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV59422.1"
                     /translation="MALDILIVDDERDICDLVAGVMEDEGYEARTASDSDSALEAIRQ
                     RRPSLALIDVWLQGSRLDGLGLVEAIKAFDPTLPIIVISGHGGLDTAVAAIRRGAFDF
                     IEKPFEASRLLHLVARATESERLKFEYEQLREKAGPSDELTGTSAAINNVRATLKRVA
                     GTGSRVLITGAPGVGKEVAARVLHGWSGRHNAPFRVISSARMDPETVETELFGSEAED
                     GSIRVGLLEQAHGGTLFLDEVADMPLTTQGKILRVLTDQSFARVGGRTMIRVDVRIIS
                     GSARDLMTEIAEGRFREDLYYRLNVVPVHIPPLRERRDDIASLCDHYIRRYAADRRVP
                     PPEISAEAMAALQAHEWPGNVRELRNVIERVMILAPSDRLARIDADMLPAELVRGGTD
                     ILPNSESITAIPLKEARENFEREYLRIQINRFSGNISRTATFIGMERSALHRKLKLLG
                     LTESSEGEG"
     gene            4794..5390
                     /gene="hfq"
                     /locus_tag="VN034_02135"
     CDS             4794..5390
                     /gene="hfq"
                     /locus_tag="VN034_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWV59423.1"
                     /translation="MPHIAFQKKVLKAGGNRHRTPRLYRQKQEANVSDKNQNLQDIFL
                     NALRKSKTPVTMFLVKGVKLQGIITWFDNFSLLLRRDGQSQLVYKHAISTVMPSHDFD
                     LSLLGDNLRDAPASKGKALQDVFLNAVRRSDESVTMFLVNGVMLQGDIVAFDLFCMLL
                     ERERQVQLVYKHAISTVQPNGPINLTDNGEGDGEADDA"
BASE COUNT         1008 a         1769 c         1803 g          950 t
ORIGIN      
        1 cagccacggg ctggtcgggc gcgcgccggc gatgcaggcg gtctaccgca ccatcgcgcg
       61 cctcgcgtcg aacgaccttg cggtcctcat cctcggcgaa tctggcactg gcaaggaagt
      121 cgtcgcgcgc gcgatccacg cgaccgggct gcgccggggc gggcccttcg tcgcgatcaa
      181 catggccgcg atcccgcgcg agttgatcga agccgagctg ttcgggcacg aaaagggggc
      241 atttaccggc gcgcacagcc gcaatgcggg gcgcttcgag caggccgcgg gcggcacgct
      301 cttcctcgac gaaatcggcg atatgccgct ggaggcacag acgcggctgc tccgcgtgct
      361 ccagagcaac gaatattcga ccgtcggtgg cagtcaggca ctgcgcgccg acgtccgcgt
      421 catcgcggcg acgcaccgcg acatgcgcac gctcgtcgcc gatggccgtt tccgcgagga
      481 tctcttttac cgcctcaacg tcatcccggt cacgctgccg ccgctgcgcg accgccgcag
      541 cgacatcgcc gcgctcgtcg gtcatttcgt cgatgcgggg cgcagctcgg gccttccgca
      601 tcgccaattc gctcccgccg cgatgcagtg gctcgaacgc tatgactggc ccggcaacgt
      661 ccgcgaactg ggcaatgtcg tccagcggct cgccgtgctg tcgcgcgata cggtggtgac
      721 ggtgcgcgat gtcgaaacgg tgcttcgcga aaatggcgag gggggcgggg cggtcgagcc
      781 cgccgcgctg atcgcgcgcg ccgtcgacga ctggacgcgc gagcagatgg cgggttcgac
      841 gaacgacggc gacatccacg aacagctcga ggccatcgtc gaggcggcgc tcttccgccg
      901 cgtcctgcgc gaagtgcgcg gcaatcagct ggaggcggcg cggcgactcg gtatcaaccg
      961 caacacgctg cgcaagcggc tcggacagct cgacatcgat ccggcgcatc cctgaacggg
     1021 aaataatccc gaaatcggtg caattgggcg acatttgcat ggattgtgtg ttttctatgc
     1081 aacaggattg tcgtaacgcg gcaacaatgg cgcgatcgac ttccctggcc ctcgacatgg
     1141 attcggacga agaggaagca cgctcgggct tctggcgctt tggaacgccc gaatattaca
     1201 cgctgggcat catcgtcagc gtcgcgatcg ccacctatat cttcgtcacc ggcgacgcgc
     1261 agagcgagag attgctgaca ccggcgctgg tggcggcgat catggtcgtc aacctcgtgc
     1321 cggcgatggc gctgatcgtg ctgatcggca gccgcgtcgc gcgcgcgcgc gcgacgcgtt
     1381 cgatgggagg ggggaacggc cgcctccacg tccggctcgt cgcgctgttt tcgctgatcg
     1441 cggcggcgcc gaccttactc gtcgtgatct tcgcttcgtt gctcttccag ttcggcgtcg
     1501 atttctggtt ctccgaccgg tcgcgcggta tgtttgaaaa tgccgccagt ctcgccgaag
     1561 gctattatca ggaaaatcag cgccaagtcg gcgcgaacac ctttgccatg gccaacgacc
     1621 tcggcacgcg attgtcccgc ttctcgatcg atagccccgg tttcaccaac tattatctgc
     1681 aacaggtcgt cgtccgcagc ctcaatgaat cggcgatcat cgagatcggc agggatggcg
     1741 tcgcgcgcac cgcggcagcg atcgaccccg acgatcgttc ggccgagaac cggctgagtt
     1801 cggagatggt cggccgcctc gacgccggcg aagatgtggt cgtggtccgc caagccaacc
     1861 ggatcgaagc cgcggcgcag ctccccggaa ccgatcgtgc ttatgtctat gcctcgcgcg
     1921 acaccaatgt tccggggttt cagcagtcgg cgcgcgcgag tgcggtgctg gccgactaca
     1981 acggcctgtt cgaacggtcg cagttgctcc agctccagtt caacggcgcg ctctatctgg
     2041 ggtcgctgct gctgctcgcg ctcgcggtga tcgccgcgat cgtcgtcgcc gaccgcatcg
     2101 tccggccgct cgggacgctg atcggcgcga cgcgcacggc ggcggatggc gatctgtcgg
     2161 tccgtgtgac tccgccggtc cgcgatgacg aaatcgcggt actgacccgc gctttcaacc
     2221 gcatgaccga gcagttggag gggcagaccg gtgcgctcgt cagcgtcaac gagcaattgg
     2281 agacgcggcg cagcttcatc gaagcagtgc tgagcggtgt atcgtccgcc gtggtctcgg
     2341 tcgacgccgg tggccgcatc ctgctggcga atgccgccgc cgagcggttg atcggccaat
     2401 cggcggaaga tctcaccggc cagccgctcg acgatgtggc gcccgaactc gcgcggttgt
     2461 tgacggagga agagcgcgaa gggatcgtgc aactgtcgcg cccggacgcc gaacccgcga
     2521 cgctcgccgc caaggcggtg gcgcagggcg acggtttcgt cctcagcttc gaggatatca
     2581 cgcagcaatt gctcgatcag cggcgcgccg cctggtccga cgtggcgcgg cgcatcgcgc
     2641 acgagatcaa gaatccgctg accccgatcc aactcgccgc cgagcggctc cagcggcgtt
     2701 tcggcgacaa ggtcgagggc gacgcggcga cattccgcaa gctcaccgac acggtgatcc
     2761 ggcaggttca cgacatgcgg cggatggtcg acgaattttc gagcttcgca cggatgccca
     2821 agccgacctt cggcgtcgag gatgtacgcg acatattgcg gcaggcggtc ttcctgttcg
     2881 aggtcgcaaa acccgacatc gccttttcgg tcaagacgcc agccgagatc gagccgctcg
     2941 tctgcgatcg ccgctccatg tcgcaagcgg taacaaatat cgtcaaaaat gcggtagaag
     3001 ccattgaaga aaaatataaa aactccgatt ccgctgcgac aggccacatc agcgcagcgc
     3061 tcgacatcgg cgcgggtggg gaggtgatca ttcgcgtcac cgacgacgga atcgggctgc
     3121 ccgaggcgcg cgatgcgatc gccgaacctt atatgacgac gcgccagggc gggacgggcc
     3181 tcggcctcgc aatcgtcaag aagatcgtcg agcaacatta tggcgaacta gaattttctg
     3241 acaatccggc ggggcgggga acgtgcgtga ccctgacgct ccatcccgac cggctccggc
     3301 ccttggccgg aaagggcggg gagcagaata tggggcagaa tgaaacggtc ccggggcgca
     3361 tccggaaccg gcaggacaga gaagatcatg gcgcttgata ttctgatcgt cgacgacgaa
     3421 cgcgacattt gcgacctcgt tgccggggtc atggaggatg aaggttatga ggcgcgcacg
     3481 gcgtccgaca gcgattcggc gctcgaggcg atccgccagc ggcggccgtc gctcgcgctg
     3541 atcgacgtct ggctgcaggg ctcgcggctc gacggcctcg gtctcgtcga agcgatcaag
     3601 gcgttcgatc cgacgctgcc gatcatcgtg atttcgggcc acggcggcct cgacaccgcg
     3661 gtcgcggcga tccggcgcgg cgcgttcgac ttcatcgaaa agccgttcga ggcgtcgcgc
     3721 ctgctccacc tcgtcgcgcg cgcgaccgaa agcgaaagac tcaagttcga atatgaacag
     3781 ctgcgcgaaa aggcggggcc atccgacgaa ctgacgggaa cgagcgccgc gatcaacaat
     3841 gtccgcgcga cgctgaagcg cgtcgccgga acgggaagcc gcgtcctgat caccggcgcg
     3901 cccggcgttg gcaaggaagt cgccgcgcgc gtgttgcacg gatggagcgg gcgacacaat
     3961 gcgccgttcc gcgttatttc gtcggcgcgc atggatcccg agacggtcga aaccgaattg
     4021 ttcggatccg aagccgagga tgggtcgatc cgcgtcggac tgctcgaaca ggcgcatggt
     4081 gggaccctgt tcctcgacga ggtggccgac atgccgctga cgacgcaggg caagatcctg
     4141 cgcgtgctca ccgatcagag ctttgcccgc gtcggcggcc gcacgatgat ccgcgtcgac
     4201 gtccgcatca tttcgggttc ggcgcgcgac ctgatgaccg aaatcgccga gggccgcttt
     4261 cgcgaggatc tttattaccg gctcaacgtc gtgcccgtgc acatcccgcc gctacgtgag
     4321 cggcgcgacg atatcgcgag cctctgcgac cattatatcc ggcgctatgc cgccgaccgc
     4381 cgcgtcccgc cgcccgagat cagcgccgaa gcgatggcgg cgctgcaggc acatgaatgg
     4441 ccgggcaacg tccgcgaact gcgcaacgtg atcgagcgcg tgatgatcct cgcgccgagc
     4501 gaccggttgg cgcggatcga tgccgatatg cttcccgccg aactggtgcg cggcgggacc
     4561 gacatcctgc ccaattcgga atcgatcacc gcaatcccgc tgaaagaggc gcgcgaaaat
     4621 ttcgagcgcg aatatctgcg tatccagatc aaccgctttt cgggcaatat ttcgcggacc
     4681 gcgaccttca tcgggatgga gcggtcggcc ttgcaccgca aactgaaact tttgggcctt
     4741 accgagagtt cggaaggcga aggttaggct ttgcgctaga cgtcccacgc gctttgccgc
     4801 atattgcgtt tcagaagaag gtcttgaaag ccggaggaaa ccggcaccgt accccgcggt
     4861 tataccgcca aaaacaggag gcaaatgtgt ccgataaaaa ccagaatctc caggatattt
     4921 tcctcaacgc cctccgcaag agcaagaccc cggtgaccat gttcctggtc aagggtgtca
     4981 agttgcaggg catcatcacc tggttcgaca atttctcgct gctgctccgc cgcgacgggc
     5041 agtcgcagct ggtgtacaag catgccatct cgacggtgat gccgtcgcac gatttcgacc
     5101 tgtcgctgct cggcgacaat ctgcgcgatg cgccggcgag caagggcaag gcgcttcagg
     5161 atgtgttcct taacgccgtc cgccgttcgg atgagtcggt cacgatgttc ctcgtcaacg
     5221 gcgtgatgct gcagggcgac atcgtcgcat tcgacctctt ctgcatgctg ctcgagcgcg
     5281 aacggcaggt gcagctcgtc tacaagcacg caatttcgac cgttcagccg aacgggccga
     5341 tcaacctcac cgacaatggc gagggtgacg gcgaagcgga cgacgcctga tcgacccgat
     5401 aatcgacaat gaagacgagg taacgcgcgg agcccccgcg cttgtcatcg tgcccgaatg
     5461 gcatagccag cggctcgcgc gcgacctcga cgcgcgcgcc gaggaggcgc gggggcttgc
     5521 gctcgcgatc
//
LOCUS       DATMSU010000079        17534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181724,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000079 DATMSU010000000
VERSION     DATMSU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 17534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17534
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181724"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VN034_02140"
     CDS             <1..692
                     /locus_tag="VN034_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV59424.1"
                     /translation="REGGGWRLDGAKSVVGWAAEADTILITARTGDDGLSLFRIDPAK
                     AEGLSLSPYRTIDGHPAADLSFENHTLSADALIGSEGGAIDLLEEARDRALALLAAEA
                     VGVLDRLLEQTVAYTGQRRQFGQPIAGFQALQHRMVDMYLECELARSSAWLAVEALDQ
                     QAPGRGRAISSAMVNIARACRFVGQQAIQLHGGMGMTDELPIGHYVKRAMAIDVLWAD
                     ADWHLARIAGL"
     gene            complement(735..2159)
                     /locus_tag="VN034_02145"
     CDS             complement(735..2159)
                     /locus_tag="VN034_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV59425.1"
                     /translation="MDFTNDYTLTIDGAAADTAKQVDVINPATGKAFASAPCAGQAEL
                     DLAVAAAKRALPAWKALGWEGRRDLLVKAAKAIEPHLDQFTALFVAEQGRPTALAKGE
                     VQMGAWWLKSVARQELANEIVEDSETRQVIVKHEPLGVVAAIVPWNFPFLLAMWKIAP
                     ALLAGNTMVLKPSPYTPLCSLKLAELWRDILPPGVYNVISGDDALGPLMTAHPGFAKI
                     SFTGSTATGKRVMEAASRDLKRVTLELGGNDAAIVLPDVDVEEVAPKLFFGSFYNTAQ
                     VCIATKRMYIHDDIYDEMLDAFHRLAKEAVVGDGSQQGVQYGPVQNRAQYDRVRALID
                     GARAEGLSLLQGAEVPEGDGYFIPLTLVDNPPEDAAVVTEEAFGPVLPLLRFSSIDDV
                     VARANDCDYGLAGAVWSKDIDAAVAVADRIETGTVWINDNFQNGPHIPFAGAKQSGFG
                     VENGADGLREFTFPKVIFVPKKSA"
     gene            2272..4407
                     /locus_tag="VN034_02150"
     CDS             2272..4407
                     /locus_tag="VN034_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV59426.1"
                     /translation="MSESPATRLRPGEDGMHFTFCRLCEAHCGLAAKVEDGRITDVQP
                     DKENPHSQGHVCRKGITFHNVTYDPDRVLTPMKRIGGPGEFVSVGWDEALDDIAARLG
                     AVIDTHGPDAVASYKGNPIAFGIDVSFSLKAFLGTLGITKYYGAGSQDTNARLTACYL
                     AFGSAVTYEIPDLPHNDMLLMFGANPLVSNGSMVWSPRVRHDLDAIAERGRVVVVDPR
                     RTETARRYQHVAVRANGDVWLLLGLLRVLIDEKLYDAAYVDAHSNGFTDLAGAVTQYA
                     LADCAERAGVEADVIAELARSFAATPRAVAYGRLGVCRGPHATLENFLLIALNLLGGK
                     LGRKGGAIFSRTILGGSQKVLTGGYGDTRSRVGDFPSVAQFLASAMLPADILEPGEGQ
                     VRAMVLTGGNMVMSAPGGQALVDALQQLDLIVALDLYQTESNRFADYILPVPTFFERD
                     DYPMAGLGSMIRPFVQVTDAVIPPVGQARSEWEIFNAILTRMGKPTHGRPMDDLDKAF
                     RLGPAGDQMGTREGWSFDALREIPHGVMVDLPDPYDPWPRIAWPDGRALLWHEMIAGE
                     FARLTPAPSDRLRLITHRDIRSLNSWLHNVPRLVRSQHPDLLMHPGDAARLDLADGEA
                     VELSTGTATVAVRVAITEDMRPGTVSYPHGWGHHGGWRHANAQGGSNINWLLPVGEQA
                     VEAISGTTIMDGLEVSIRKLAAIAETTAA"
     gene            4513..5121
                     /locus_tag="VN034_02155"
     CDS             4513..5121
                     /locus_tag="VN034_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV59427.1"
                     /translation="MDSGPAAARTDDNSTQERILRAAYDCFEQYGIAKTTIENIASRA
                     KVSRPTVYKYYPSKDAILDEISVRETWRVNSEVRRRLVRSDDFADFLTDTLLLVIRLA
                     NENVYIRRMVESIEFQESVISPSSLMQQLQRTWWTNLFEQARARGEIAADLDIDEIIY
                     WLARAQAMLMLQVASPQIDDAEKRRFIRRFIVQPLLAGSEIR"
     gene            5229..6005
                     /locus_tag="VN034_02160"
     CDS             5229..6005
                     /locus_tag="VN034_02160"
                     /inference="COORDINATES: protein motif:HMM:NF022586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HWV59428.1"
                     /translation="MTDDLAGRMPANPRYGGGYFVRSTVFRRRASDRVEAVLEDPFHA
                     LALAIHHDHRALTAVESRWERHPLSSCAGAERALSGLAGLPLIADLRTRWLQTEPALQ
                     CTHMFDTLRLAAAHIAQGRDDRRYDVILPDADADTQPVRLLQDGAPVLAIEIDAEFRI
                     TAPSTYAGSPLLAGFARWATERLSAEEYERMFLIQRGAFVGRGQRMDITQYYGRAGAL
                     SGPPPAACFGSQPERYAASIRLANARPGLRREDAARFPFA"
     gene            6025..7626
                     /locus_tag="VN034_02165"
     CDS             6025..7626
                     /locus_tag="VN034_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWV59429.1"
                     /translation="MSDMPFPTADQAITRHLLERQAREHPDDLYMRFQDGSEWSFAQA
                     LDQAHRAAAALKRLGVEQGDHVFNWLPNGKAAVRAWLGTHMLGAVFVPANLAYRGSLL
                     RHVLWLADAKVGLAHRDLMPRLADVERGMLEQVIVIGGDADPVEGLILHGEDMLTEPG
                     SAGDLARPLAPWDNMAIIFTSGTTGPSKAVMCSYAHMWTFYHAAMPAMIGREYRHLAH
                     LPIFHSGGFGCLYGQLSNAGSVVLVESFKTDQFWQIVREHQANTTTLLGAMIPFLLST
                     PPSDDDRNHNLKSVSAVPYTADVKAFTARFGVGAFAAYSMTELANPVQSPLNPDKIGV
                     AGRLRAGQHIRLVDEHDIEVPDGAVGEAILRCDQPWTMFTEYYKNPEATAEAWRNGWL
                     HTGDLLRRDADGDYFFVDRRKDAIRRRGENISAYEVEQEIRAFPGVKEAAVIGVASDK
                     TEDEVMACIMPTEGAEIDLKALTDFLATRLPHYMVPRYFRLMPDLPRTPTQKIQKYAL
                     RDDGITADTWDREAAGIRLRRERIG"
     gene            complement(7598..9205)
                     /locus_tag="VN034_02170"
     CDS             complement(7598..9205)
                     /locus_tag="VN034_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWV59430.1"
                     /translation="MVDTLNMPSVPVLIRERAARDPSAPLITDVAGGTMTGGEFHESA
                     LRVADAMAALGIAPGECVATMLDPCLAAYCAWIGLGWRGALEVPINPEYKGNLLAYAL
                     NDCRARVLITSNACVDQVDAVRDRLETLERIITIDDEVCSSHISIATLTSVEAGAPRI
                     EHDEPGLADTNAVIYTSGTTGPSKGVLQAWGSIQQVQHNFSGEVIDPDQVPVYYSPWP
                     TFHSSGRVGFVFAAVRGGHMVFRSRFSVRHYWADVREHGCTHTQLMGIAGFLMNVERR
                     PGDADNPLQWVLMNPVLPDYRDFEARFGVRVCTGWGMTEIGFPLNASDLPNAQTCGKL
                     SPLYEVRIVDAQGRDLPDGQAGELLIRGKAPFLITKGYLNKPEANRKAWRDGWYATGD
                     ILRRDPDGYFYFLDRANDYLRVRGNNVSSLELEGEVRAHPAVAEAAAIGVKAADVPAL
                     PGNARADEDEIKIAVKLNGSAPLGEGELVDYLAERLPRYMVPRFVEFVDDLPRTPTGK
                     IRKAGLRESPVNAATWDRLSRSVPAAI"
     gene            9377..10582
                     /locus_tag="VN034_02175"
     CDS             9377..10582
                     /locus_tag="VN034_02175"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWV59431.1"
                     /translation="MYLSPEHNRQVAEKLFRHIRDNSTDMIEETYEYPLSVYSDPDVA
                     IAEREKIFEHYPMMALHGSQLPESGSYVTVKLNRSEALVTRGKDGQVRAFLNMCRHRG
                     ATLVTQAEGKQGRFSCPYHGWTYTNEGELIGLTFKQTLGLSLPCRERNLIALPCEERH
                     GFVWIVENPEGSIDVAAHLGPVMDGVLAGYGLDRQYFYREAFFDFAQNWKIMVDGLTD
                     GYHVQWVHGPTIRPYFYMNMMARVEGTGDHAVTTTPRRKIDEILGSEPGEHDLDPYVV
                     YGNMVFPNVSIQLHPHHAEFWTMYQDVHNPGRSRVHLRFLTPKAPGDYDEKGQTILAK
                     NWDIAEAAILNEDVPVGDSIQRSANMPNTGTMLLAHNEVINQQFHRSYDRVMAGPSVV
                     SMKPRAARG"
     gene            complement(10599..11648)
                     /locus_tag="VN034_02180"
     CDS             complement(10599..11648)
                     /locus_tag="VN034_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWV59432.1"
                     /translation="MLAASAPATIADISADWIGRSLAGRFPGARVAAARMEGFVDGTA
                     QKCRFELDYDKAGPSGPPSLWVKGGFDPKGASQGNAFSNEVRFYRDLAPLLDISLPDS
                     FYGAIDKATNNGVVVLEDLLLRGARFGRATEPLTPDMAARVLTIQARLHARFWDGRDT
                     AHLDWLVPGGAIAGAGVVDQYFGLWDNARKLPRFAFLTAEQTDRPRMQAALNRLIDHL
                     RDHPLCVLHGDSQGGNLFFIDDAPGYLDWQHSMIGHWAFDLAGFMMTSLTVEDRRLHE
                     RALLQHYLSELTDAGVVAPSFGAAWDDYARYAMWAFMWVMCPVEAHPEAVCSANSERA
                     CAAISDLETLAKLGV"
     gene            complement(11648..12556)
                     /locus_tag="VN034_02185"
     CDS             complement(11648..12556)
                     /locus_tag="VN034_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59433.1"
                     /translation="MHKVTIEDCRRHQPDGDPRWQESFYVGWVDLENRACGANHISLA
                     PAGHRDTHVWSWTMVDGEVIGRSQAHGMPMPDSDYDDMRIGALHVRSSDDPRRLALGV
                     DYANGVTLDLNFAASCDPVELSLDRGDVVLGDRHYELMGQVSGTLRTPSRSIALQASG
                     WSDHSWGARDFSTNISHRWLWASFGADFAISVFGFVSNEGYFPFGWVWDGGEIHLVTA
                     VRFDAVVADDGITPRGCDARIATASGRRYRVKGRVRDAALMGGVGWHAMNGLTEYECG
                     GRIGEGFLEVNELKVLTAAMKAELAL"
     gene            complement(12559..13407)
                     /locus_tag="VN034_02190"
     CDS             complement(12559..13407)
                     /locus_tag="VN034_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59434.1"
                     /translation="MPAFSDGEVILVTGATRGVGEAVARRCAKAGARVAVTGRNVERG
                     EAVCAAIASDGGDALFVPIDVTDEAAVARAFDQCLQHWGRLDGCVANAADLSLGALDG
                     PVTEITLDGWNRLITADLTSVFLTAKHAIRAMLKGGRGGALLTIGSLAGVRGNIGHDA
                     YSAAKGGIVALTRSIAAYYARYDIRCNCLNLGFVDSGSDRIETILGHPGFRRQLLDFH
                     LGAWGRADDIGGIAALLLSGEGRYINGASIPVDGGAYAASHMPRPTVAAMIPGFPPLR
                     STPDKD"
     gene            complement(13429..15573)
                     /locus_tag="VN034_02195"
     CDS             complement(13429..15573)
                     /locus_tag="VN034_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59435.1"
                     /translation="MRSRSRHSRSCRALVAGFAIQPFLWISPAFGQTQAAAEDSGADS
                     GDIVVTAQRRSERATDVPISITALGADTLEAAGVLSTGDLGKVTPGLSMDRAGPFSQP
                     TIRGIGSSVTGPGINTSVATYIDGYYQPSTVSSDFSLADVASVQVLKGPQGTLFGRNS
                     TGGAILVTTLAPSFDPMARARISYASYDDITATAVVSTGLTDKIAVLLSGLYRHDRGY
                     ARDVVTNAPLQKAREYVVRGKVLFRPSDDVSLTLAYTHGDVDDPWGVAQVAYRGVSAA
                     AGIPGVIVPDRPKEVAATLPPESRVNYDAVTFTADVDLGGVNIKSYTGYRDERDYVQL
                     DSDKTAFAFQYIDFRPVDETFTQEIDISSTGNGPLQWVAGLYYYHDEAGYLDLGVTQG
                     GPRFTFLNARLKSEAYAAFADVTYEVSPGLYLTGGVRYNNEKVTETFDSILSNYVPAS
                     FSKTFENLSPRAVIRFEPTASSSVYLSYNQGYKAGTFNPTGLSPTPVRPEKIDAFEAG
                     YKMRSGGTLFEVAGYYYDYKDLQFVSYVGATAQLTNAAKARIYGAEVNLEQDVGDHLT
                     LSANAAWTDAKYRDFTGANHYEYLGNGVIVNGPDDASGNPMVRTPKFAARGAIEFHAP
                     LAGGEFRANAAVKYQSRIRFDPFGETGQGQYALVDARVAWTDPSEHVTIAAFGTNLTN
                     KDYYAVVTQQSESWPANYGHPRRFGVELSFRY"
     gene            complement(15778..16815)
                     /locus_tag="VN034_02200"
     CDS             complement(15778..16815)
                     /locus_tag="VN034_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HWV59436.1"
                     /translation="MHLATLSAVKLPNHSIKLMAIMLDELGADVERALQQVGLTASDI
                     EDPAALVTGLQEVEFQRAFMQQTPDQPEVWVDLGSRYRLLTHAHTDYGLLMSTAPTVD
                     AAIKIGFTYGDLYYMLADAEGIFAEDGRQIGFRSLPVELPRDMRRFSALRDVGVNCTV
                     FSELWGEPFPFDTVELPVPARDEPFVRRFLPDADLRFDSEFNRWRWSVRLDQRRPPHS
                     DPVLHDYYRSACDRLLAAAHREGDLVDRVLTLLDESKGQDSVDYAARKLALSTRSLQR
                     KLGDRGFSYRELAALARHRHACRLLLTTRHPVSRVALDTGYDNVSAFNNAFRRLSGVT
                     PSQYRNGMAVV"
     gene            complement(16969..>17534)
                     /locus_tag="VN034_02205"
     CDS             complement(16969..>17534)
                     /locus_tag="VN034_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWV59437.1"
                     /translation="ADCSAHWMPEALDPLLNPDATFARPGFLDASGKMRFWARMENYR
                     CADGKLIFLMTFTPKSWQALLGLIGRPDLDEIYTREPRTGTEDAEVNAALIPIFASRP
                     RADWLADFARDNVAAMPVNSFADVIADPHFRARENTYRVALHDGRDLTLTSSAIHVGG
                     QDFTMPLAPELGEHDDAIRDEFGLGDG"
BASE COUNT         3191 a         5843 c         5508 g         2992 t
ORIGIN      
        1 gccgcgaagg cggcggctgg cggctcgacg gggcgaaatc ggtcgtcggc tgggcggccg
       61 aggccgatac gattctcatc accgcgcgca ccggcgacga cggcctgtcg ctcttccgga
      121 tcgatcccgc caaggccgaa ggcctcagcc tctcacccta tcgcacgatc gacggccatc
      181 ccgccgccga cctgtcgttc gaaaatcata ccctctccgc cgacgcattg atcggctccg
      241 aaggcggagc catcgacctg ctcgaggaag cgcgagaccg cgcgctcgcg ctgctcgcgg
      301 cggaggcggt gggcgtgctc gaccgcctgc tcgaacagac ggtcgcctac accggccagc
      361 ggcgccagtt cggccagccc atcgccggct ttcaggcgct gcagcaccgg atggtcgaca
      421 tgtatctcga atgcgagctg gcgcgttctt cggcatggct ggcggtggaa gcgctcgacc
      481 aacaagcccc cgggcgcggg cgcgcgatat cgagcgcgat ggtcaatatc gcccgcgcct
      541 gccgtttcgt cggccagcag gcgatccagc ttcacggcgg gatggggatg accgacgagc
      601 tgccgatcgg ccattatgtc aagcgcgcga tggcgatcga cgtgctgtgg gcggatgccg
      661 actggcacct cgcccgcatc gccgggctct agggcggcgc gcgcatgccg gagccgcgcg
      721 cccttaaccg cccgtcaggc cgacttcttg ggcacgaaga tcaccttggg aaaggtgaat
      781 tcgcgcaggc catccgcccc gttttcgacg ccgaaccccg actgtttcgc gccggcgaaa
      841 ggaatgtgcg ggccgttctg gaaattgtcg ttgatccaca ccgttccggt ctcgatccgg
      901 tccgcaaccg cgaccgccgc gtcgatatcc ttcgaccaca ccgcgcccgc gaggccgtag
      961 tcgcaatcgt tcgcgcgcgc gacgacatcg tcgatgctcg aaaagcgcag caaaggcagc
     1021 accgggccga acgcctcctc ggtcaccacc gcggcatcct cgggcgggtt gtcgacgagc
     1081 gtgagcggga tgaaatagcc atcgccctcg ggcacctcgg cgccctgcag caagctcagc
     1141 ccctcggcgc gcgcgccgtc gatcagcgcg cggacccggt cgtactgcgc ccggttctgc
     1201 accgggccat attgcacgcc ttgctgcgat ccgtcgccga ccacggcttc cttcgccagc
     1261 cggtgaaagg catccagcat ctcgtcatag atgtcatcgt ggatatacat gcgcttggtg
     1321 gcgatgcaga cctgcgcggt gttgtagaag gagccgaaga acagcttcgg cgcgacctct
     1381 tccacgtcga catcgggcag tacgatcgcg gcgtcgttgc cgccgagctc gagcgtgacg
     1441 cgcttgaggt cgcgcgacgc cgcttccatc acccgcttgc ccgtcgcggt cgatccggtg
     1501 aagctgatct tcgcaaagcc cggatgcgcg gtcatcagcg ggccgagcgc atcgtcgcca
     1561 gaaatgacgt tgtagacgcc gggcggcaga atatcgcgcc acagttcggc gagcttcagc
     1621 gaacagagcg gggtgtaggg cgagggcttc agcaccatcg tattgcccgc gagcagcgcc
     1681 ggcgcgatct tccacatcgc cagcaggaac ggaaaattcc acggcacgat cgccgcgacg
     1741 acgccgagcg gctcgtgctt gacgatcacc tgccgcgtct cgctgtcctc gacgatttcg
     1801 ttggccagtt cctgccgcgc gaccgatttc agccaccacg cgcccatctg gacttcaccc
     1861 ttcgcgagcg cggtcggccg cccctgctcg gcgacgaaca aggcggtgaa ctggtcgaga
     1921 tgcggctcga tcgcctttgc cgccttgacc agcaggtcgc gccgcccttc ccagccgagc
     1981 gccttccacg cgggcagcgc gcgcttcgcc gcggcgacgg ccagatcgag ctcggcctgc
     2041 ccggcacaag gcgccgacgc aaaggccttt ccggtcgcgg gattgatcac atcgacctgc
     2101 tttgcagtat cggccgcggc gccgtcgatg gtcagcgtat aatcgttggt gaagtccatt
     2161 tttccctcgc cctgcctgat cggaaatcca gcttttccaa accggtttgc accatttgcc
     2221 cggataatca aatactatta gataatgagc ggaatttatg aggagaaggc gatgagcgag
     2281 agtccggcga cgcggctgcg gcccggtgag gacggcatgc atttcacctt ctgccgtttg
     2341 tgcgaagcgc attgcgggct tgcggcaaag gtcgaggacg ggcgcatcac cgacgtccag
     2401 cccgacaagg agaatcccca ttcgcagggg cacgtctgcc ggaaggggat caccttccac
     2461 aatgtcactt acgatcccga tcgcgtcctg accccaatga agcgcatcgg cggcccgggc
     2521 gagttcgtgt ccgtaggctg ggacgaagcg ctggacgaca tcgccgcgcg gctgggcgcc
     2581 gtgatcgata cgcacggccc cgacgcggtc gcgtcgtaca agggcaatcc gatcgccttc
     2641 ggcatcgacg tgtcgttcag cctcaaggcg ttcctgggga cgctgggcat caccaaatat
     2701 tatggcgcgg ggtcgcagga caccaatgcg cggctgaccg cctgctatct cgccttcggg
     2761 tcggcggtaa cctatgagat tccggacctg ccgcataacg acatgctgct catgttcggc
     2821 gccaacccgc tggtgtcgaa cggatcgatg gtctggtcgc cgcgcgtgcg ccacgatctc
     2881 gacgcgattg ccgagcgcgg gcgggtcgtc gtcgtcgacc cgcgccgcac cgagaccgcg
     2941 cgccgctacc agcatgtcgc ggtgcgcgcg aacggcgacg tctggctgct gctcgggctg
     3001 ctccgcgtcc tgatcgacga aaaattgtac gatgccgcct atgtcgacgc gcatagcaat
     3061 ggctttaccg atctcgccgg cgcggtgacg caatatgcgc tggctgattg tgccgagcga
     3121 gcgggcgtcg aggccgatgt gatcgccgag ctcgcgcgca gcttcgccgc aacgccgcgc
     3181 gcggtcgcct atggccgcct cggcgtctgc cgcgggccgc acgcgacgct cgagaatttc
     3241 ctgctgatcg cgctcaacct gctcggcggc aaattgggac gaaagggcgg cgcgatcttc
     3301 agccgcacga tcctcggcgg atcgcaaaag gtgctgaccg gcggttatgg cgacacgcgc
     3361 agccgcgtcg gcgacttccc cagcgtcgcg caattcctcg cctcggcgat gctgccggcg
     3421 gacatattgg agccgggcga ggggcaggtg cgcgcgatgg tcctgaccgg cggcaatatg
     3481 gtgatgtcgg cgccgggcgg gcaggcgctg gtcgacgcac tgcagcagct cgacctgatc
     3541 gtcgcgctcg acctctacca gaccgagagc aatcggtttg ccgactatat cctgcccgtg
     3601 ccgaccttct tcgagcgcga cgactatccg atggcggggc ttggatcgat gatccgtccc
     3661 ttcgtccagg tcaccgacgc ggtcatcccg ccggtgggcc aggcacgctc cgaatgggag
     3721 attttcaacg caatcctgac ccgaatgggc aagccgaccc acggccggcc gatggacgat
     3781 ctcgacaagg ccttccgcct cggtcccgcc ggtgaccaga tggggacgcg cgaaggctgg
     3841 agcttcgatg cgctgcgcga aatcccgcat ggcgtgatgg tcgacctgcc cgatccctat
     3901 gatccttggc cgcggattgc gtggcccgat ggccgcgcct tgctgtggca cgagatgatc
     3961 gccggcgaat tcgcgcgact gacacccgcg ccgtccgaca ggctgcgcct gatcacccac
     4021 cgcgacatcc gctcgctcaa cagctggctg cacaatgtcc cgcgcctcgt ccgcagccag
     4081 catcccgacc tgctgatgca tccgggcgat gctgcgcggc tggaccttgc cgatggcgag
     4141 gccgtcgagc tgtcgacggg caccgccacc gtcgccgtcc gcgtcgcgat taccgaggat
     4201 atgcggcccg gcacggtcag ctatccgcac ggctggggcc atcatggcgg ctggcggcac
     4261 gctaatgcgc agggcggcag taacatcaac tggctgctgc ccgtcggcga gcaagcggtc
     4321 gaagcgattt cgggaacgac gatcatggac gggctcgaag tatcgatccg gaaactggct
     4381 gccatcgctg aaacaaccgc cgcctgagag gcgccggcgg cgccgcgtga cgattttgcg
     4441 acttgttcgc actgccagcc ttgctaacga cggcgcgatg cagcattccc cgtcgggggc
     4501 aggagatcga cgatggacag cggcccggcg gcagcccgga cggacgataa ttcgacgcag
     4561 gagcgcatcc tgcgcgccgc ctatgattgt ttcgagcaat atgggattgc gaagacgacg
     4621 atcgagaata tcgcgagccg cgccaaggtg tcgcggccga ccgtctataa atattatccg
     4681 agcaaggatg cgatcctcga cgagatttcg gtgcgcgaga cgtggcgcgt caatagcgaa
     4741 gtgcggcggc ggctcgtacg ctccgacgat tttgccgatt tcctgaccga tacgctgctg
     4801 ctcgtcatcc ggttggcgaa cgagaatgtc tatatccgcc gcatggtcga aagcatcgag
     4861 tttcaggaat cggtgatttc gccgagcagc ctgatgcagc agttgcagcg cacctggtgg
     4921 accaatttgt tcgaacaggc gcgcgcgcgc ggcgagatcg cggccgacct cgatatcgac
     4981 gagatcattt attggctcgc gcgcgcgcag gcgatgctga tgcttcaggt cgcctcgccg
     5041 cagatcgacg atgccgaaaa gcgccgcttc atccggcgct tcatcgtcca gcctttgctc
     5101 gccggcagcg aaatccggta acgccttcat cgccgacttg acggcctccc gccaagctga
     5161 cacagatatt taatcaattg attaagtagc cgcacgacca agcggcgggc atgggagatc
     5221 cgcgaccgat gaccgatgat ctggcgggcc ggatgcccgc caatccccga tatggcggcg
     5281 gctatttcgt ccggtcgacg gtgtttcgcc gccgcgcctc cgatcgggtc gaggcggtgc
     5341 tggaggatcc gttccacgcg ctggcgctgg cgatccatca cgaccatcgc gcgctgaccg
     5401 ccgtggaaag ccgctgggaa cgccatccgc tgagcagctg cgcgggtgcc gagcgtgcgc
     5461 tgtccggcct tgccggcctg ccgctgatcg cggatctgcg cacgcgctgg ctgcagaccg
     5521 aaccggcgct gcaatgcacc catatgttcg acacgctgcg cctcgccgcc gcgcacatcg
     5581 cgcaaggtcg cgatgatcgg cgttacgacg tcatcctgcc cgacgccgat gccgacacgc
     5641 aaccggtacg cctccttcag gatggcgcgc ccgtgctggc aatcgagatc gatgccgaat
     5701 tccggatcac cgcgccttca acctatgccg gctcgccgct gctcgcgggc ttcgcgcgct
     5761 gggcgaccga gcggctgtcg gcagaggaat atgaacgcat gttcctgatc cagcgcggcg
     5821 ccttcgtcgg acgcggacaa cgcatggaca tcacgcaata ttacggacgc gcgggcgccc
     5881 tgtcgggccc gccgcccgcc gcctgcttcg gctcgcaacc cgaacgctac gccgcttcca
     5941 ttcgcctcgc caacgcacgc cccggtctcc gccgcgagga tgccgcccgc ttccctttcg
     6001 cctgactgct gcaggaaaga accgatgtcc gacatgccct tccccaccgc cgatcaagcg
     6061 atcacccgcc atctgctcga aaggcaggcg cgcgagcatc cggacgatct ctacatgcgc
     6121 tttcaggacg gcagcgaatg gagtttcgcc caggcgctcg atcaggcgca tcgcgccgcg
     6181 gccgcactca aaaggctggg cgtcgaacag ggcgaccatg tcttcaactg gctgcccaac
     6241 ggcaaggcgg ccgtgcgcgc ctggctcggc acgcatatgc tcggcgcggt cttcgtgccc
     6301 gccaatctcg cctatcgcgg cagcctgctc cggcacgtgc tgtggctcgc cgacgcgaag
     6361 gtcgggctgg ctcaccgcga cctcatgccg cgcctcgccg acgtcgagcg cggcatgctc
     6421 gaacaggtga tcgttatcgg cggcgacgcc gaccccgtcg aaggcctcat cctccacggc
     6481 gaggatatgc tgaccgagcc cggaagcgcg ggcgacctcg cccggccgct cgcaccgtgg
     6541 gacaatatgg cgatcatctt cacctcgggc accaccggcc cgtcgaaggc ggtgatgtgc
     6601 tcctatgccc atatgtggac cttctaccac gccgcgatgc cggcgatgat cggacgcgag
     6661 tatcgccacc tcgcgcatct gccgattttc cattcgggcg gcttcggctg cctttacggc
     6721 cagctctcga acgccggctc ggtcgtgctc gtcgaatcgt tcaagaccga ccagttctgg
     6781 cagatcgtcc gcgagcatca ggcgaatacg acgacgctgc tcggggcgat gatccccttc
     6841 ctgctcagca ccccgccgtc ggacgacgac aggaaccaca acctgaaatc ggtgagcgcg
     6901 gttccctaca ccgccgacgt caaggcgttc accgcgcgtt tcggggtcgg cgccttcgcc
     6961 gcctacagca tgaccgagct cgccaatccg gtgcaaagcc cgctcaaccc cgacaagatc
     7021 ggcgtcgccg ggcggctgcg cgcggggcag catatccggc tggtcgacga acatgacatc
     7081 gaggtgcccg acggcgcggt cggcgaggcg atcctgcgct gcgaccagcc ctggacgatg
     7141 ttcaccgaat attacaaaaa tccggaggcg acggccgaag cctggcgcaa cggctggctg
     7201 cacaccggcg atctcttgcg ccgcgacgcc gacggcgatt atttctttgt cgaccgccgc
     7261 aaggacgcga tccggcgccg cggcgagaat atctcggcct atgaggtcga acaggaaatc
     7321 cgcgcctttc ccggcgtcaa ggaagccgcg gtgatcgggg tcgcgagcga caagaccgag
     7381 gatgaggtga tggcgtgcat catgcccacc gaaggcgccg agatcgacct caaggcgctg
     7441 accgacttcc tcgcgacgcg cctgccgcat tatatggtgc cgcgctattt ccggctgatg
     7501 cccgacctgc cgcgcacccc gacgcagaag atccagaaat atgcgctgcg cgacgacggc
     7561 atcaccgccg acacctggga ccgtgaggcg gccgggatca gattgcggcg ggaacggatc
     7621 ggctgagccg gtcccacgtc gccgcgttca ccgggctttc gcgaaggccc gccttgcgga
     7681 tcttgcccgt cggcgtgcgc ggcaggtcat cgacgaactc gacgaagcgc gggaccatat
     7741 agcgtggcaa ccgctcggcg agataatcga caagttcgcc ctcgccgagc ggcgccgagc
     7801 cattcagttt caccgcgatc ttgatctcat cctcgtcggc ccgcgcattg cccggaagcg
     7861 cggggacatc ggcggccttc accccgatcg ccgcggcttc ggcgaccgcc gggtgcgcgc
     7921 gcacctcgcc ttccagctcc aatgacgaga cattattgcc gcgcacgcgg aggtaatcgt
     7981 tggcgcggtc gaggaagtag aaatagccgt cgggatcgcg gcgaagaatg tcgcccgtcg
     8041 cataccagcc gtcgcgccac gccttccggt tcgcctcggg cttgttcaga tagcccttcg
     8101 tgatcaggaa gggcgccttg ccgcggatca gcaattcgcc ggcctgcccg tcgggaaggt
     8161 cgcggccttg ggcatcgacg atgcggactt catagagcgg cgacagcttg ccgcaggtct
     8221 gcgcgttcgg caggtccgat gcgttgagcg gaaagccgat ttcggtcatc ccccatccgg
     8281 tacagacccg gaccccgaac cgcgcctcga aatcgcgata gtcgggcagc accgggttca
     8341 tcagcaccca ttgcagcgga ttgtcggcat cgcccggccg ccgctcgacg ttcatcagga
     8401 aaccggcgat ccccatcagc tgcgtgtggg tgcagccatg ttcgcgcaca tcggcccaat
     8461 agtggcggac cgaaaagcgg ctgcgaaaca ccatatgccc gccgcgcacg gccgcgaaaa
     8521 cgaagcccac gcgtcccgac gaatggaagg tcggccaagg cgaataatag accggcactt
     8581 gatcgggatc gatcacctcg ccgctgaaat tatgctgcac ctgctggatc gaaccccacg
     8641 cctgcagcac gcctttcgag gggccggtgg tgcccgacgt atagatgacg gcattggtgt
     8701 cggcgagtcc aggctcgtcg tgctcgatgc gcggtgcacc cgcctcgacc gaggtgagcg
     8761 tcgcgatcga tatatggctg gagcagacct catcgtcgat cgtgatgatc cgctcgagcg
     8821 tttcaagccg gtcgcgaacc gcatcgacct gatcgacgca ggcgttgctg gtgatcagca
     8881 cccgcgcgcg gcagtcattg agcgcatagg ccaacagatt gcccttatat tcgggattga
     8941 tcggcacctc gagcgcgccg cgccagccaa gcccgatcca cgcgcaatag gcggcgaggc
     9001 acggatcgag catcgtcgcg acgcactctc cgggcgcgat ccccagcgcg gccatcgcgt
     9061 cggcgacacg caatgccgat tcgtgaaact ccccgccggt catcgtaccg cccgcgacgt
     9121 cggtgatgag cggcgccgac ggatcgcgcg ctgcgcgctc acgaatcagc accgggaccg
     9181 aaggcatatt cagcgtatcg accatatctc tccccgcgct tcgccttgcc gatcgaccga
     9241 catgtaaagt cgcattttcc acaaacccct ttttatatag gtttttgcta tttacaatgc
     9301 tacatatgtg taaaggcaac tgctccacat atcgcgtcga gccgttgctt tccgatcagg
     9361 aaatgcggag agtcgaatgt atctgagccc cgaacataac cgtcaggtgg cggaaaagct
     9421 gttccggcat atccgcgaca acagcaccga tatgatcgag gaaacctatg aatatccgct
     9481 ctcggtctat tcggatccgg acgtcgcgat cgccgagcgc gagaaaatct tcgaacatta
     9541 tccgatgatg gcgctgcacg gcagccagct tcccgaatcg ggcagctatg tgacggtgaa
     9601 attgaaccgc agcgaagcgc tggtcacgcg cggcaaggac ggccaggtcc gcgccttcct
     9661 caacatgtgc cgccaccgcg gcgcgacgct ggtgacgcag gccgagggta agcaggggcg
     9721 cttctcctgc ccctatcacg gctggaccta caccaacgag ggcgagctga tcggcctcac
     9781 cttcaagcag acgctggggc tcagcctgcc gtgccgcgaa cgcaatctga tcgcgctgcc
     9841 gtgcgaggag cgtcacggct tcgtctggat cgtcgaaaat cccgaagggt caatcgacgt
     9901 cgccgcgcat ctcggccccg tcatggacgg cgtgctcgcg ggatatggcc tcgaccgcca
     9961 atatttctat cgcgaggcct ttttcgactt cgcccagaac tggaagatca tggtcgacgg
    10021 gctgaccgac ggctatcacg tccagtgggt ccatggcccg acgatccgac cctatttcta
    10081 catgaacatg atggcgcgcg tcgaaggcac cggcgaccat gccgtcacga cgacgccgcg
    10141 gcgcaagatc gacgagatat tgggcagcga accgggcgaa cacgacctcg acccctatgt
    10201 cgtctacggc aatatggtct tccccaatgt cagcatccag ttgcacccgc atcatgccga
    10261 attctggacc atgtatcagg acgtccataa ccccggccgc tcgcgcgtcc acctgcgctt
    10321 cctgacgccc aaggcgcccg gcgactatga cgaaaagggt cagacgatcc tcgccaagaa
    10381 ttgggacatc gccgaagccg ctatcctcaa tgaggacgtg ccggtcggcg acagcatcca
    10441 gcgctcggcg aacatgccca acaccggcac gatgctgctc gcgcacaacg aggtcatcaa
    10501 ccagcaattt caccgcagtt acgaccgggt gatggcggga ccgagcgtgg tgtcgatgaa
    10561 gccgcgcgcc gcgcgcggct gacgaggcgc cgccttcgct acacgccaag cttggcgagt
    10621 gtctcgaggt ccgaaatcgc cgcacaggcg cgttcgctgt tcgccgaaca cacggcttcg
    10681 ggatgcgcct ccacggggca catcacccac atgaaggccc acatggcata acgcgcataa
    10741 tcatcccatg cggcgccgaa agatggcgcg acgaccccgg catcggtcag ttccgacaga
    10801 taatgctgca gcagcgcgcg ctcatggagc cgccggtcct cgaccgtcag cgaggtcatc
    10861 atgaatccgg caaggtcgaa ggcccaatgg ccgatcatcg aatgctgcca gtcgagatag
    10921 cccggcgcat cgtcgatgaa aaacaggttt ccgccctgtg aatccccgtg gagcacgcac
    10981 agcggatgat cgcgcaaatg atcgatcagc cggttcagcg ccgcttgcat ccgcggcctg
    11041 tcggtctgtt cggcggtaag gaaggcgaaa cgcggcagtt tccgggcatt gtcccacagg
    11101 ccgaaatatt gatcgaccac gcccgcgccg gcgatcgctc cgccggggac gagccagtcg
    11161 agatgcgccg tatcgcggcc gtcccagaat cgcgcgtgga gccgcgcctg tatcgtcagg
    11221 acgcgcgccg ccatatcggg ggtcagcggc tcggtcgccc gcccgaagcg cgcaccacgc
    11281 agcagcaggt cctccagcac gaccaccccg ttgttcgtcg ccttatcgat cgcgccgtaa
    11341 aagctgtcgg gcaggctgat gtcgagcaaa ggcgcgagat cgcggtagaa gcgcacttcg
    11401 ttggaaaagg cattcccctg actggcccct ttcggatcga acccgccctt gacccacagc
    11461 gacggcgggc cgctggggcc ggccttgtca tagtccagtt cgaaccggca tttttgggcg
    11521 gtgccgtcga cgaacccttc catgcgtgcc gccgcgacgc gcgcgccggg gaaacgtccg
    11581 gcgagcgagc gtccgatcca gtcggcggag atatccgcaa tcgtcgcggg tgcgctggcc
    11641 gccaacatca gagcgccagc tccgccttca tcgcggcggt caggaccttc agttcgttga
    11701 cttccagaaa gccctcgccg atgcggccgc cgcattcata ctcggtcagc ccgttcatcg
    11761 cgtgccagcc gacgcccccc atcagcgccg catcgcggac gcggcccttg acgcggtagc
    11821 ggcggccgga tgccgtcgcg atacgcgcat cgcatccgcg cggggtgatg ccgtcgtcgg
    11881 cgaccaccgc gtcgaaacgc accgccgtca ccagatggat ctcgccgccg tcccacaccc
    11941 agccaaaggg gaaatagcct tcgttgctga cgaatccgaa cacgctgatc gcgaaatcgg
    12001 cgccgaagga cgcccaaagc cagcggtgcg agatattggt cgaaaaatcg cgcgcccccc
    12061 agctatggtc cgaccagccc gacgcctgga gggcgatcga gcgcgatggc gtccgcagcg
    12121 tccccgacac ctgacccatc aattcataat gccggtcgcc gagcaccaca tcgccgcgat
    12181 cgagcgacag ttctacggga tcacaactgg ccgcgaagtt caggtcgagc gtgacgccgt
    12241 tcgcatagtc gacgccaagc gccagccgcc gcggatcgtc gctcgaccgg acatgcaggg
    12301 cgccgatcct catatcgtca taatcgctgt cgggcatcgg catgccgtgc gcctgcgatc
    12361 ggccgatgac ctcgccgtcg accatggtcc agctccacac atgggtgtcg cggtggccgg
    12421 cgggcgcgag gctgatgtga ttggcgccgc aagcccgatt ctccaagtcg acccaaccga
    12481 cgtagaagct ttcctgccag cgcggatcgc cgtccggctg atggcggcgg cagtcttcga
    12541 ttgtgacctt gtgcatgatc aatccttgtc gggggtggag cggaggggag ggaagccggg
    12601 aatcatcgcc gccaccgtcg gccgcggcat gtggctggcg gcataggcgc cgccgtcgac
    12661 ggggatgctc gcgccgttga tgtatcggcc ctcgcccgaa agcagcaacg ccgcgatacc
    12721 cccgatgtcg tcggcccggc cccaggcacc gagatgaaaa tcaagcagtt gccggcgaaa
    12781 gcccggatgg ccgagaattg tttcgatccg gtcggatcct gaatcgacaa agccgagatt
    12841 caggcagttg cagcggatgt cgtagcgcgc ataataggcg gcgatcgagc gcgtcagcgc
    12901 gacgataccg ccctttgcgg cgctataggc atcgtgaccg atgttgccgc gcacgcccgc
    12961 gagcgatccg atcgtcagca gcgcgccgcc acgcccgccc ttgagcatcg ccctgattgc
    13021 atgttttgcg gtcagaaaaa ccgacgtcag atcggcggtg atcagccggt tccagccgtc
    13081 cagcgtgatt tcggtgaccg gcccgtcgag cgcgcccaag ctcagatcgg cggcgttggc
    13141 gacacagccg tcgagccgcc cccaatgctg gaggcattgg tcgaaagcgc gcgcgacggc
    13201 tgcctcgtcg gtcacgtcga tcggcacgaa cagcgcgtcg ccgccgtcgg aggcgatggc
    13261 ggcacacacc gcctctcccc tttcgacgtt acgccccgtg acggcgaccc gcgcgccggc
    13321 cttcgcgcag cgacgcgcta ccgcttcccc gacgccgcgt gtggcgccgg tgaccaatat
    13381 gacctcgcca tcggaaaagg ctggcatcgt gcggaccttc gcgagccgtc agtaccggaa
    13441 ggacagttcg acaccgaaac ggcgcggatg accatagttg gcgggccagc tttccgattg
    13501 ttgcgtgacg accgcgtaat aatccttgtt ggtcagattg gtcccgaacg cagcgatcgt
    13561 cacatgctcg gacgggtcgg tccaggccac gcgcgcatcg acgagcgcat attgaccctg
    13621 cccggtttcg ccgaacgggt cgaagcggat ccggctctga tatttgaccg ccgcattggc
    13681 ccggaattcg ccgccggcaa gcggggcatg aaattcgatc gcaccgcgcg ccgcgaactt
    13741 gggagtgcga accatgggat tgcccgacgc atcgtcggga ccgttcacga tcacgccgtt
    13801 gcccagatat tcataatggt tcgcgccggt gaaatcgcga tatttggcat cggtccaggc
    13861 ggcgttcgcc gacaatgtca gatggtcgcc gacatcctgt tccagattga cctcggcgcc
    13921 atagatgcgc gccttcgccg cattggtcag ctgcgcggtc gcgccgacat agctgacgaa
    13981 ctgcagatcc ttgtaatcat aataatagcc cgcgacctcg aacagggtgc cgcccgaccg
    14041 cattttgtat cccgcctcga aggcgtcgat cttttcgggc cgcaccggcg tcgggctgag
    14101 accggtggga ttgaaagtcc ccgccttata tccctgattg taggacagat agacgctgct
    14161 gctcgcggtg ggttcgaagc ggatgaccgc acgcgggctc agattttcga atgttttgga
    14221 aaaagatgcg ggaacatagt tcgacaggat cgaatcgaac gtctccgtca ccttctcatt
    14281 gttgtagcgc accccgccgg tcagatagag gccgggcgat acctcgtagg tgacatcggc
    14341 gaaggcagcg taagcctccg atttgagccg cgcgttgaga aaggtgaaac gcggcccgcc
    14401 ttgcgtcacg ccgaggtcga gatagcccgc ctcgtcatga taataataaa ggccggcgac
    14461 ccattgcagc ggtccatttc cggtcgagga aatatcgatt tcctgcgtga acgtctcatc
    14521 gacaggacga aaatcgatat actgaaaagc aaaggcggtc ttgtcgctgt cgagttgcac
    14581 ataatcgcgc tcgtcgcgat agcccgtgta gctcttgatg ttcacgccgc cgagatcgac
    14641 gtcggcggtg aaggtcacag catcatagtt gaccctggat tcgggcggca gggtcgcggc
    14701 gacctccttt ggcctgtccg gcacgatcac gcccgggatg ccggccgccg ccgacacgcc
    14761 gcgataggcg acctgcgcga cgccccacgg atcatcaacg tcgccgtggg tataggcgag
    14821 ggtcaagctg acatcgtccg aaggcctgaa caggactttc ccgcggacca catattctct
    14881 cgccttctga agcggcgcat tggtcacaac atcgcgggca tagccgcgat catgtcgata
    14941 aaggcccgag agcagcacgg cgatcttgtc ggtgagcccg gtcgacacca cagccgtcgc
    15001 ggtgatgtcg tcatagctcg catagctgat ccgcgcgcgc gccatcggat cgaaactcgg
    15061 cgcgagtgtc gtcaccaata tcgcgccgcc cgtcgaattg cgtccgaaaa gcgttccctg
    15121 cggccccttc agcacttgga cggaggccac gtcggcaagg ctgaaatcgc tggaaacggt
    15181 gctcggctga taatagccgt cgatataggt cgccacgctc gtgttgatgc ccggaccggt
    15241 caccgacgac ccgatcccgc ggatcgtcgg ctgcgaaaag ggacccgcgc ggtccatcga
    15301 cagccccggg gtcaccttgc ccagatcgcc cgtggacagc acgccggccg cctcgagcgt
    15361 atcggcgccg agcgcggtga tggagattgg aacgtcggtc gccctttcgg aacggcgctg
    15421 cgcggtgacg acgatatcgc cgctatcggc gccgctatcc tccgccgccg cctgcgtctg
    15481 cccgaaagcg gggctgatcc ataagaacgg ctggatcgcg aagcccgcga ccaacgcgcg
    15541 gcacgacctg ctgtggcgac tcctggaacg catctgcctc tccctaagca cacagcccgg
    15601 ccgggacttt gcccggcgta aaggctggtc atcggccacg acattctctg tcacgggttc
    15661 gcgcggaacg tctgcggccg acctttcatt agtcaccgga caataggtgc gcgatgcgcg
    15721 ggcgcgccaa aatcttcaca ttttgcgcca gtcagccccg acgatagcgg ggtcgcttca
    15781 gactacggcc atgccgttgc gatactggct gggcgtgact ccggacaggc gacggaaggc
    15841 gttgttgaag gccgagacat tgtcataacc cgtatcgagc gcgacacggc tcacgggatg
    15901 gcgggtcgtg agcaacagac ggcaagcgtg tctgtggcgc gcaagcgcgg ccagttcgcg
    15961 ataggaaaat ccccggtcgc ccagcttgcg ctgcaggctg cgcgtcgata gcgccagttt
    16021 tcgcgccgca tagtcgacgc tgtcctgccc tttgctctcg tcgagcaggg tcagcacgcg
    16081 gtcgacgagg tcgccttccc gatgcgccgc ggccaacagc cggtcgcagg cgctgcgata
    16141 ataatcgtga agcaccggat cgctgtgcgg cggccgccgc tggtcgaggc gcaccgacca
    16201 acgccagcga ttgaactcgc tgtcgaaacg gaggtcggca tcgggaagga agcggcgcac
    16261 gaacggctca tcgcgcgccg gcaccggcaa ttcgacggta tcgaacggaa agggctcccc
    16321 ccatagttcg gaaaagaccg tgcaattgac cccgacatcg cgcaacgccg aaaagcgccg
    16381 catatcgcgg ggtaattcga ccggcagcga acggaacccg atctgccggc cgtcctcggc
    16441 gaaaatgccc tcggcatcgg ccaacatgta gtaaaggtcg ccgtaggtga agccgatctt
    16501 gatcgccgca tcgacggtcg gcgcggtcga catcaacagg ccgtaatcgg tgtgggcgtg
    16561 ggtcagcagc cggtatcgcg atcccaggtc cacccagact tcgggctggt cgggcgtctg
    16621 ctgcatgaac gcccgctgga actcgacctc ttgcaatccc gtgaccagcg cggctggatc
    16681 ctcgatatcg ctggccgtca atcccacctg ttgcaacgcc cgctcgacgt cggcgcccag
    16741 ttcgtcgagc atgatcgcca tcagcttgat gctatggttg ggcaacttaa cggcggacag
    16801 cgtcgccaga tgcatcgttt ttcctcgcca ttagcgatct gggcggcggc ggtccggcct
    16861 cgcccgaccg aaaaggtcgc gcatccgcca ccgttcgtca atcgattggt taacggatgc
    16921 cgctacaagg atcgctaagg tccgatcgag ccggcccgcc gagacccgtc agccgtcgcc
    16981 cagcccgaat tcgtcgcgga tggcgtcatc atgctcgcct agttcggggg ccagcggcat
    17041 cgtgaaatcc tggccgccga catggatcgc cgacgaggtc agcgtaagat cacggccatc
    17101 gtgcagcgcg acccgatagg tattttcgcg cgcacggaaa tgcgggtcgg cgatgacgtc
    17161 ggcgaaactg ttcaccggca tcgccgccac attgtcgcgc gcgaaatcgg cgagccagtc
    17221 ggcgcgcggg cgcgacgcga agatcgggat caacgcggcg ttgacctcgg catcctccgt
    17281 cccggtccgc ggctcgcgcg tgtaaatctc atcgagatcg ggccggccga tcagccccag
    17341 cagcgcctgc cagctcttcg gagtgaaggt catcaggaag atcagcttgc cgtcggcgca
    17401 gcggtaattc tccatccgcg cccagaagcg catcttgccg ctcgcatcga ggaaacccgg
    17461 tcgcgcgaag gtagcatcgg gattgagcag cgggtcgagc gcctcgggca tccagtgcgc
    17521 cgaacagtcg gccg
//
LOCUS       DATMSU010000080         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_105944,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000080 DATMSU010000000
VERSION     DATMSU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105944"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /gene="aat"
                     /locus_tag="VN034_02210"
     CDS             complement(<1..566)
                     /gene="aat"
                     /locus_tag="VN034_02210"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWV59438.1"
                     /translation="MADGADDPSVHWVEPRLRAILPLGDFHLSRSLKKVIVSDRFRVT
                     TDAAFADMVALCAEPAGDRPTTWINPVIKASYDRLFRLGHAHSVECWLDGELVGGLYG
                     VSLGRAFFGESMVSRVRDASKVALAHLVARLIAGGWKLLDCQFITPHLASLGAIEIRQ
                     ADYLARLYSVLAGGDGGASLGGATGAGAG"
     gene            complement(894..>1583)
                     /locus_tag="VN034_02215"
     CDS             complement(894..>1583)
                     /locus_tag="VN034_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV59439.1"
                     /translation="TPANVARDPYRHPAETLAFFGVKPGDTVVELWPGGGWYTEILAP
                     LTKAGGGTLYAAAPWERGLNKVKEKQAADAATYGAVKLAEFPNAGTNPKVPDGSADVV
                     LTFRNVHNWRSGGAENTANAFKQIFAMLKPGGTLGIAEHRLNENDDSAKEEKSGYLKE
                     STVIALAEAAGFKLAGRSEINANPKDTKDYPKGVWTLPPSLTEGETDRAKYVAIGESD
                     RMTLKFVKPAS"
BASE COUNT          259 a          509 c          518 g          297 t
ORIGIN      
        1 ccggcgcccg ctccggtcgc gccgcccaag ctcgcgccgc cgtcaccccc cgccaacacc
       61 gaatagagcc gcgcgagata gtcggcctgg cggatctcga tcgcgccgag gctcgcgagg
      121 tgcggggtga tgaactggca atcgagcaat ttccagccgc ccgcgatcag ccgcgcgacg
      181 agatgcgcga gcgcgacctt cgacgcgtcg cgcacgcggc tgaccatcga ttcgccgaaa
      241 aaagcgcggc cgagcgacac gccatagagg ccgccgacga gctcgccatc gagccagcat
      301 tcgacgctgt gcgcatggcc gagccggaac agccggtcgt agctcgcctt gatgacgggg
      361 ttgatccatg tcgtcggccg gtcgcccgcg ggttcggcgc agagcgcgac catgtcggca
      421 aaggcggcat cggtggtgac gcgaaagcgg tcggagacga tgaccttctt caggctgcgc
      481 gacagatgga agtcgcccag gggcaggatc gcgcgcagcc gcggctcgac ccagtggacc
      541 gacgggtcgt ccgccccatc ggccatcggg aaaatcccct gcgcataggc gctcaaaagc
      601 agcgccgggt cgatggactc gaaatgtcgg gtcacagtcc gctcttcacg cggctgaggc
      661 gtcaaaatgg cgaagatatc gggcggaaat gcccgccggg aacagtggtt ctgttcccaa
      721 gggcatttcc gctcgagatc gaagccattt tgacgtccct tcgggatttg accgattttg
      781 tccatggctg cgtcagcgag tcttggaata tcgacatatg cctgcgtctc gcttccttgc
      841 cctgaacaaa atcgcttcaa acctcagccg cgtgaagagc ggactgtgag ccgttagctt
      901 gcgggcttta cgaatttcag cgtcatgcga tccgattcgc cgatcgcgac atatttcgcg
      961 cggtcggtct cgccctcggt caggctcggc ggcagcgtcc agacgccctt gggataatcc
     1021 ttggtgtcct tcgggttggc gttgatctcg ctgcggccgg cgagcttgaa ccccgccgcc
     1081 tcggcaagcg cgatgaccgt gctttccttg agatagcccg atttctcttc cttcgcgcta
     1141 tcgtcattct cattgagccg gtgctcggcg atgcccagcg tgccgccggg tttgagcatc
     1201 gcgaagatct gtttgaaggc gttcgcggtg ttttcggcgc cgcccgatcg ccaattgtgg
     1261 acgttgcgga aggtcagcac gacgtcggcg ctgccgtcgg ggactttggg gttggtcccc
     1321 gcgttcggaa attcggcgag tttcaccgcg ccataggtcg cggcatcggc ggcctgcttt
     1381 tccttgacct tgttcagccc gcgctcccac ggcgccgcgg cataaagcgt gccgccgccg
     1441 gccttggtca gcggcgcgag gatttcagtg taccagccgc cgcccggcca cagctcgacg
     1501 accgtgtcgc cgggcttcac gccgaaaaag gcgagcgttt cggcgggatg gcgatagggg
     1561 tcgcgcgcga cgttcgccgg ggt
//
LOCUS       DATMSU010000081         5395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_77290, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000081 DATMSU010000000
VERSION     DATMSU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77290"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /gene="miaB"
                     /locus_tag="VN034_02220"
     CDS             <1..1055
                     /gene="miaB"
                     /locus_tag="VN034_02220"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWV59440.1"
                     /translation="RAPSVDVVVGPQAYHRLPDLIARAERGEKAVDLDMPLESKFGAL
                     PKRAGGQRPTAFLTVQEGCDKFCTYCVVPYTRGAEISRPFADLIAEARALVAGGAREI
                     TLLGQNVNAWDGEDDKGRAIGLDGLIRELAREDGLERIRYTTSHPNDMTDGLIAAHGE
                     VDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPDIAISGDFIVGFPG
                     ETEADFQATLDIVRATGYAMAYSFKYSRRPGTPAATMDGQISEAEMNDRLQRLQALLN
                     EQQQAFNEATVGRTTRLLLERKGKLEGQLIGKSPWLQSVHVIAPGLKIGDMVDVRITS
                     AGPNSLGAELLMEAVA"
     gene            1186..2193
                     /locus_tag="VN034_02225"
     CDS             1186..2193
                     /locus_tag="VN034_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWV59441.1"
                     /translation="MSKRPLTASTPAEPGDRVRLTAEFERTQLLGILFGEFDRNLVAI
                     ENRLGVYISARGNRVQIEGEAEAAARARDVLTDLYDRIEKGQEVDAGMVDGLIAMSAQ
                     PTLDGIIRHDTSEAPPVMIRTRKKTIVPRAPSQVPYMQALARDEVIFALGPAGTGKTY
                     LAVAQAVAQLITGSVQRLILSRPAVEAGEKLGFLPGDMKEKVDPYLRPLYDALHDCLP
                     AEQVERRIASGEIEIAPLAFMRGRTLADAFIILDEAQNTTIPQMKMFLTRFGMNSRMV
                     ICGDPKQVDLPMPATSGLADAVARLEGLEGINVSRFTAADVVRHPIVGRIVEAYEGPG
                     A"
     gene            2625..4853
                     /locus_tag="VN034_02230"
     CDS             2625..4853
                     /locus_tag="VN034_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HWV59442.1"
                     /translation="MTSHHLPSVSRLAAGSALGLALAATAAPAFAQDNDGEYWLARKN
                     QIVITATRTAVKAEDLPVTVSVKTDEQIADELVTDIRDLVRFEPGVSVQRQPARFGAA
                     LGATGRAGNDSFNIRGIGGNRVLIQVDGVRVPDGFSFGAQASGRGDYVDLGLVKSVEI
                     LRGPSSALYGSDGLAGAVSFVTADPADFIEAGKNVGGLLRAGYSSADEEFSETAILAG
                     RSGDWSLMAAYTRRDYKELENQGSLGDEGGTGATRTRANPQDGRSDAALARIVYDPAN
                     GHKLRLTGEYLDTHLYTNGLSGLGPGLVGTVDRLEGWDTGKRQRVSLDWSWEGEGAID
                     FARVALYWQDGEDRQYTEEDRTPSADRTRLNSFENRVFGASADARADFETGAITHRLV
                     FGGDISKTRQRGLRGGTVPPAGETYPTRAFPSTDFTRAGLFVGDEIAIADGRLLLYPA
                     LRFDWYDLSPDNDPLLPAFQGAKQDGSRVSPKFGAVWKITDEVRLFANYATGFKAPEP
                     GQVNQFFENLTSPFFSYRTLPNPDLGPERSESFEGGVRFSSDAVSLDVTAFSARYKDF
                     ISQEEVGGTGSTADPILYQFVNLDRVRVKGAEARFEGRASSGLYTTLALSYAKGDIID
                     PAGARSPLESIDPLKLVAGVGYRAPAGRFGGQLVMTHAARKEASRTTSGYRPDSFTIL
                     DATAFVRIIDALTLRAGVFNILDKKYSWWSDARGLASTSTVTDAYTQPGRNVSASLSF
                     RF"
     gene            4874..>5395
                     /locus_tag="VN034_02235"
     CDS             4874..>5395
                     /locus_tag="VN034_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HWV59443.1"
                     /translation="MKHYRKFAAGLLLLTAALPAAASAHPPIAAEEAAANFEQDRADI
                     LAMAGNYRVSFNMQESTRWDPDYEVLEPKRSGGNEVVRVIEDTGRKIVLQHMLVITGD
                     DDKSFVIKHWRQDWEYEPAKILAYSDRNTWAWEDVSERMRTGRWSQTVYQVDDSPRYA
                     GWGQFETQGGIRRW"
BASE COUNT          962 a         1854 c         1701 g          878 t
ORIGIN      
        1 gccgtgcgcc cagcgtcgat gtcgtcgtcg gcccgcaggc ctatcaccgc ctgcccgacc
       61 tgatcgcgcg tgccgagcgc ggcgagaagg cggtcgacct cgacatgccg ctcgaaagca
      121 agttcggcgc gctgcccaag cgcgccggcg gccagcgtcc gacagccttc ctcaccgtgc
      181 aggaaggctg cgacaaattc tgcacctatt gcgtcgtccc ctatacgcgc ggcgccgaga
      241 tcagccggcc cttcgccgat ctgatcgccg aagcgcgcgc tctcgtcgcg ggcggggcgc
      301 gcgagatcac cttgctcggg cagaacgtca acgcgtggga tggcgaggat gacaagggcc
      361 gcgcgatcgg cctcgacggg ctgatccgcg agctggcgcg tgaggacggg ctcgaacgca
      421 tccgctacac gaccagtcac cccaacgata tgaccgacgg gctgatcgcc gcgcatggcg
      481 aggtcgacaa gctgatgccc ttcctccacc tgccggtgca ggcgggcagc gaccgcatcc
      541 tcgccgcgat gaaccgcagc catagcgttg aaagctatct caaggtcatc gaacgcgtgc
      601 gcgaagcgcg ccccgacatc gcgatttcgg gcgacttcat cgtcggcttc cccggcgaaa
      661 ccgaggcgga ttttcaggcg acgctcgata tcgtccgcgc gacgggctac gcgatggcat
      721 acagcttcaa atattcgcgc cgtcccggca cgcccgcggc cacgatggac ggccagattt
      781 ccgaagccga aatgaacgac cgcctccagc ggttgcaggc tttgctcaac gagcagcagc
      841 aggcgttcaa cgaagcgacc gtcggccgca ccacgcgcct gctcctcgag cgcaagggca
      901 agctcgaagg ccagctcatc ggcaagtcgc catggctgca gtcggttcac gtcatcgcgc
      961 cgggattgaa gatcggcgac atggtcgacg tccgcattac ttcggccggg ccgaacagtc
     1021 tgggcgccga gctgctgatg gaagcggtcg cctagtgcca cgggcggaag ctgtctccac
     1081 gaagacacac tccgtctttt tccgtcgcat ccccaacccg cgggagatgg actcgcgcct
     1141 tcgcgggact acagtcgcct tatcgccaga aggagccttg cccacgtgtc caaacgcccg
     1201 ctgaccgcct ccacccccgc cgaacccggc gaccgcgtcc gactgacggc ggaattcgag
     1261 cgcacacagc ttttgggcat tctcttcggc gagttcgacc gcaacctcgt cgcgatcgag
     1321 aaccgcctcg gcgtctatat ctcggcgcgc ggcaaccgcg tgcagatcga gggcgaggcc
     1381 gaagccgcgg cgcgcgcgcg cgatgtgctc accgacctgt atgaccggat cgaaaagggg
     1441 caggaggtcg atgccggcat ggtcgacggc ctgatcgcga tgtcggcgca gccgacgctc
     1501 gacggcatca tccgccacga tacgagcgaa gcgcccccgg tgatgatccg cacgcgcaag
     1561 aagacgatcg tcccgcgcgc gccgtcgcag gtcccctata tgcaggcgct cgcgcgcgac
     1621 gaggtgattt tcgcattggg tccggccggt accggcaaga cctatctggc ggtcgcgcag
     1681 gccgtcgcgc agctgatcac cggcagcgtc cagcgcctga tcctctcgcg ccccgcggtc
     1741 gaggcggggg agaagctcgg cttcctgccc ggcgatatga aggaaaaggt cgacccctat
     1801 ctgcgcccgc tctacgacgc gctccacgac tgcctgcccg ccgagcaggt tgagcgccgc
     1861 atcgcgagcg gtgagatcga gatcgcgccg ctggccttca tgcgcggccg cacgctcgcc
     1921 gacgccttca tcatcctcga cgaggcgcag aataccacga tcccgcagat gaagatgttc
     1981 ctgacgcgtt tcggcatgaa cagccgcatg gtgatctgcg gcgacccgaa acaggtcgat
     2041 ctgccgatgc ccgcgacctc gggccttgcc gacgccgtcg cgcggctcga ggggctcgaa
     2101 gggatcaacg tcagccgctt caccgccgcc gacgtggtgc gtcatccgat cgtcgggcgg
     2161 atcgtcgaag cctatgaggg gccgggggct taacgcgagc gatattcgct ttggggtggt
     2221 gagctgccgt tttcctaaac ccctcctctt cagaggaggg gcagtgagac ttggtccggc
     2281 gtagccgggc ctctagtcga acggggtggt cgtagcggga cgtgcacgcc gggccgagac
     2341 caccaccccg ctgcgactaa cgaacaagtt cgtaagtctc gctacccctc ctctgaagag
     2401 gaggggctgg cgcatatttg ccaatgttcg cctcaggtcg aaaccggcct cgaacttcag
     2461 tgaacccatg gctccttgaa aatccttccg gacgagcccc caggatgctg gcccttcata
     2521 ccggttttac gcgaacgatt cgcaatagca tcttgaccct ccgattttgc gcgcctagag
     2581 cgccgctatt gcgagtcaat cgcaagataa tcaggggaag aaatttgacc agccatcatc
     2641 tgccgtccgt cagtcggctc gccgccggca gcgcgctggg ccttgcgctc gccgccaccg
     2701 cggcgccggc cttcgcgcag gataatgacg gcgaatattg gctggcgcgc aagaaccaga
     2761 tcgtcatcac cgcgacgcgc accgcggtga aggccgaaga cttgcccgtc acggtcagcg
     2821 tcaagaccga cgagcagatc gccgacgaac tcgtcaccga catccgcgac ctcgtccgtt
     2881 tcgaacccgg cgtcagcgtc cagcgccagc ccgcgcgctt cggcgccgcg ctcggcgcta
     2941 cgggacgcgc gggcaacgac agcttcaaca tccgcggcat cggcggcaac cgcgtgctga
     3001 tccaggtcga cggcgtgcgc gtccccgacg gcttcagctt cggcgcgcag gcgtcggggc
     3061 gcggcgatta tgtcgacctc ggcctcgtca aatcggtcga gatcctgcgc ggtccttcgt
     3121 cggcgcttta cggcagcgac gggcttgccg gcgcggtcag tttcgtcacc gccgaccccg
     3181 ccgatttcat cgaagcgggc aaaaatgtcg gcggcctcct ccgcgccggc tatagcagtg
     3241 ccgacgagga gttcagcgaa accgcgatcc tcgcggggcg tagcggcgac tggtcgctga
     3301 tggccgccta tacccgccgt gattacaagg agcttgaaaa tcagggcagt cttggcgacg
     3361 aaggcggcac cggtgcgacg cgcacccgcg ccaatccgca ggacggacgt tcggatgcgg
     3421 cgctggcgcg catcgtctat gatcccgcca acggccacaa gctgcgcctg acgggcgaat
     3481 atctcgacac gcacctctat acgaacggcc tcagcggcct tggcccgggg ctcgtcggca
     3541 ccgtcgatcg gctcgaaggc tgggacacgg ggaaacgcca gcgcgtctcg ctcgactgga
     3601 gctgggaagg cgagggggcg atcgatttcg ctcgcgtcgc gctctactgg caggacggcg
     3661 aggaccggca atataccgag gaagatcgca cgccgtcggc cgaccgcacg cgtctcaata
     3721 gcttcgagaa ccgcgtcttc ggcgcttcgg ccgatgcgcg cgccgatttc gaaaccggcg
     3781 cgatcaccca ccgcctcgtt ttcggcggcg acatcagcaa gacgcgccag cgcggcctgc
     3841 gcggcggcac cgtgccgccg gcgggcgaga cctatccgac gcgcgctttc ccgagtaccg
     3901 acttcacccg cgccgggctg ttcgtcggcg acgagatcgc gatcgccgat ggccgcctgc
     3961 tgctctatcc ggcgctgcgc ttcgactggt acgacctgtc gcccgacaat gatccgctgc
     4021 tccccgcctt tcagggcgcg aaacaggacg gatcgcgcgt ttcgcccaaa ttcggcgcgg
     4081 tgtggaagat caccgacgag gtgcggctgt tcgccaatta tgcgaccggc ttcaaggcgc
     4141 ccgaaccggg gcaggtgaac cagttcttcg aaaatctgac ctcgcccttc ttttcatacc
     4201 gcacgcttcc caatcccgat ctcgggcccg agcgcagcga aagcttcgag ggcggcgtgc
     4261 gcttttcgag cgacgcggtc agtctcgacg tcaccgcctt ttcggcgcgc tacaaggatt
     4321 tcatcagcca ggaagaggtc ggcggcacgg gatcgaccgc cgaccccatt ctctatcaat
     4381 tcgtgaatct cgatcgcgtc cgggtcaaag gagccgaggc gcggttcgag gggcgagcgt
     4441 cgtccggcct gtatacgacg ctcgccctat cctatgcgaa gggtgacatc atcgaccccg
     4501 ccggcgcgcg ctcgccgctg gaatcgatcg acccgctcaa gcttgtcgcc ggggtcggct
     4561 atcgcgcgcc cgcggggcgt ttcggcggcc agcttgtcat gacccacgcc gcccgcaagg
     4621 aagcgtcgcg gaccacgagc ggctaccgcc ccgacagctt cacgatcctc gacgcgaccg
     4681 cctttgtccg gatcatcgac gcgctgactc tccgcgccgg cgtcttcaac atcctcgaca
     4741 agaaatacag ctggtggagc gatgcacgcg gcctcgcctc cacctcgacc gtcaccgacg
     4801 cctataccca gcccggccgc aatgtgagcg cctcgctgag cttccgcttc taaagaagaa
     4861 aaggactgca ccgatgaaac attatcgcaa gttcgccgcc gggctgttgc tgctcaccgc
     4921 cgcgctgccc gccgccgctt ccgcccaccc gccgatcgcc gccgaggagg ccgccgccaa
     4981 tttcgagcag gaccgcgccg acatcctcgc gatggcgggc aattaccgcg tcagcttcaa
     5041 catgcaggaa tcgacgcgct gggatcccga ttatgaagtg ctcgaaccca aaaggtcggg
     5101 cggcaatgag gtggttcgtg tgatcgagga cacggggcgc aagatcgtgc tccagcatat
     5161 gctcgttatc accggcgacg acgacaagag cttcgtcatc aagcattggc ggcaggactg
     5221 ggaatatgag cccgcgaaaa tcctcgccta ttcggaccgc aacacatggg catgggaaga
     5281 cgtctccgaa cggatgcgca ccggtcgctg gtcgcagacg gtctatcagg tcgacgacag
     5341 cccgcgctac gccggctggg gccagttcga aacgcagggc ggcatccgcc gctgg
//
LOCUS       DATMSU010000082         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172263,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000082 DATMSU010000000
VERSION     DATMSU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172263"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VN034_02240"
     CDS             <1..304
                     /locus_tag="VN034_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HWV59444.1"
                     /translation="SDGAIGKLIGTTRNTIGAIRDRSHWNSANIVPKDPVTLGLCSQR
                     ELDALVAKAAKKAGIKAPEDSRFEGDREALLEELRAERSAAAEARAAEEAEADGEA"
     gene            306..1445
                     /locus_tag="VN034_02245"
     CDS             306..1445
                     /locus_tag="VN034_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV59445.1"
                     /translation="MSGADEDNIPAASGSPDASLTQDDSFTATSHAGLTYPWGEAAPG
                     PGETIRIADGISWARIPMPGSLGHINSWLLDDGANGEEGGVAVVDTGICLTMCSDAWK
                     ALYVGALRDKRITRVIGTHLHPDHIGLAGWIAKKQDVKLWMTRGEMLTARAIVADSSD
                     SAPDEVLAQSRAAGWDEDAIEAQRSRGWNMFQRMIFTLPRSYVRIADGETLDMGAHRW
                     RVVTGSGHSPEHACLWNEREGVLVSGDQVLPRISSNVSVNITEPEADPLGEWLASIDK
                     LLSVVPADVVTCPAHGEPFKGLHVRLMALRDEHRMRLYNLAEAAAKAPMRAVDSFPLL
                     FNRPIGEHNQGLATGEALAHLKRLEVEGRVKREIRDGVWWYHGVA"
     gene            complement(1464..2423)
                     /locus_tag="VN034_02250"
     CDS             complement(1464..2423)
                     /locus_tag="VN034_02250"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59446.1"
                     /translation="MRRAVLAALSVLSGALLAGPAAAETLLVGNKGEDTLSVIALDSG
                     AELARLPTGRMPHEIAISPDGKQAAVVAYGGTTIDIFDVAKRTKLKTIDISPNQRPHG
                     LLWLADGRLVATAEGSESVAVVAPDGTLTSIATGQKGTHMIAVAPDNRTAYTANIGSG
                     TVSVLDLKDGKKLRDLAIGGKPEGLALTKGGRELWVGDLDAPRVSIWDTKSGEKIAEL
                     PVDPVAIRVLASPDGKLVATSNIASGTISLFDAETRAPLKTIKVSGDAAKGQVTLLFS
                     PDSKRLYAAETGHDKVAEIDVATAKVLRRIAAGKQGDGLAIAP"
     gene            2599..2745
                     /locus_tag="VN034_02255"
     CDS             2599..2745
                     /locus_tag="VN034_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59447.1"
                     /translation="MELPSIDIAALPLLDTMVGLFGSLMSESVTSGPSVFEIAVTIAL
                     IAHD"
BASE COUNT          453 a          983 c          970 g          462 t
ORIGIN      
        1 atcggacggc gcgatcggca agctgatcgg cacgacgcgc aacacgatcg gcgcgatccg
       61 cgaccgcagc cactggaaca gcgcgaatat cgtgcccaag gatccggtga cgctgggcct
      121 ctgctcgcag cgcgaactcg acgcactcgt cgccaaggcg gcgaagaagg ccggcatcaa
      181 ggcacccgag gacagccgtt tcgagggcga ccgcgaagcc ttgctcgagg aactgcgcgc
      241 cgaacgtagc gccgccgccg aagcgcgcgc cgccgaagag gccgaagccg acggcgaagc
      301 ctgacgtgtc gggggcggac gaggacaata ttccggcggc cagcggctcg ccggacgcct
      361 cgctgaccca ggacgacagc ttcaccgcga cgagccatgc aggcctcacc tatccgtggg
      421 gcgaggcggc gcccgggccg ggcgagacga tccgcatcgc ggacggcatc agctgggcgc
      481 gcatcccgat gccggggtcg ctcggccata tcaacagctg gctcctggat gacggcgcca
      541 atggtgaaga ggggggcgtg gccgtcgtcg ataccggcat ctgcctgacg atgtgttccg
      601 acgcgtggaa ggcgctctac gtcggcgcgc tcagggacaa gcgcatcacg cgcgtcatcg
      661 gcacgcatct gcatcccgac catatcggtc tcgcgggctg gatcgcgaaa aagcaggacg
      721 tcaagctgtg gatgacgcgc ggcgagatgc tgaccgcgcg cgcgatcgtc gccgattcat
      781 ccgactccgc gcccgacgag gtgctcgccc agtcgcgcgc cgcgggctgg gacgaggatg
      841 cgatcgaggc gcagcggtcg cgcggctgga acatgtttca gcgcatgatc ttcacgctgc
      901 cgcgctccta tgtgcggatc gcggacggcg agacgctcga catgggcgcg caccggtggc
      961 gcgtcgtcac cggttcgggg cacagccccg aacatgcgtg tctgtggaac gagcgcgaag
     1021 gcgtgctggt gtcgggcgac caggtgttgc cgcggatcag ctcgaacgtg tcggtcaaca
     1081 tcaccgaacc cgaagccgac ccgctcggcg aatggctcgc gtcgatcgac aaattgctca
     1141 gcgtcgtgcc cgccgacgtc gtcacctgcc ccgcgcacgg cgagccgttc aaggggctgc
     1201 acgtccgcct gatggcgctg cgcgacgagc atcgcatgcg gctctacaat ctcgccgaag
     1261 ccgcggcgaa ggcgccgatg cgcgcggtag acagctttcc cctgctcttc aatcgcccga
     1321 tcggcgagca taatcagggc ctcgcgaccg gcgaggcgct cgcgcatctc aagcggctcg
     1381 aggtcgaagg gcgcgtgaag cgcgagatcc gcgatggcgt gtggtggtac cacggggtgg
     1441 cgtgaggccc gtcccaatac cgtctaaggc gcgattgcca gtccgtcgcc ctgcttcccc
     1501 gccgcgatcc ggcgcagcac cttggccgta gcgacgtcga tctcggcgac cttgtcgtgt
     1561 cccgtctcgg cggcatagag ccgcttcgaa tcggggctga acagcaaagt cacctgtccc
     1621 ttcgccgcgt cgcccgacac cttgatcgtc ttcagcggcg cgcgcgtttc ggcgtcgaac
     1681 aggctgatcg tccccgatgc gatattgctg gtcgcgacaa gcttgccgtc ggggctcgcg
     1741 agcacgcgga tcgcgaccgg atcgaccggc aattcggcga tcttctcgcc cgatttcgta
     1801 tcccagatcg acacgcgcgg cgcatcgagg tcgccgaccc acagctcgcg cccgcccttc
     1861 gtcagcgcca gcccctcggg cttgccgccg atggccaggt cgcgcagctt ctttccgtcc
     1921 ttcaggtcga gcacgctcac cgttcccgat ccgatattgg cggtgtaggc ggtgcgatta
     1981 tcgggcgcga cggcgatcat atgcgtgccc ttctgccccg tcgcgatcga ggtcagcgtg
     2041 ccgtccggcg cgaccaccgc gaccgactcg ctgccctcgg cggtcgcgac gagccgtccg
     2101 tcggctagcc agagcagccc gtgcggccgc tggttggggc tgatgtcgat cgtcttgagc
     2161 ttggtgcgct tcgcgacgtc gaatatgtcg atcgtcgtcc cgccataggc gaccaccgcc
     2221 gcctgtttgc catcgggcga gatcgcgatc tcgtgcggca tcctgcccgt cggcagccgc
     2281 gcgagttcgg cgccgctatc cagagcgatg acgctcagcg tatcctcgcc cttgttgccg
     2341 acgagcagcg tttcggcggc ggccggaccg gcgagcaggg cgcccgaaag gacggagagg
     2401 gcggctagga cggcgcggcg catgaacaat ctcccggttg tgacggcgca tcctatcccg
     2461 cccgcacgct accgcaaccg cgcttcgatg ggcgacatgg cgcggattac gttaagttaa
     2521 ctcggcgatc gggggccgtg cccttagccc tcttgctggc ggcaggtcgc gccgccgatc
     2581 aggaaagaag ggggaaatat ggagctgccg tcaatcgata tcgccgcgct gccgttgctc
     2641 gacacaatgg tcgggctgtt cgggtcgctg atgagcgaaa gcgtcacgtc ggggccgtcg
     2701 gttttcgaaa tcgcggtcac catcgcgctc atcgcccacg actgatcgcg ggcggggacg
     2761 ggcggcggcg cgttccgccg ctcgcccccg cgcctttttt catgaccgcc gcccgctcgc
     2821 gccacgatca tttcccccgc gccgatcgcg cgcgttgcca gcgcatcc
//
LOCUS       DATMSU010000083         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_106053,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000083 DATMSU010000000
VERSION     DATMSU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106053"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VN034_02260"
     CDS             <1..194
                     /locus_tag="VN034_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59448.1"
                     /translation="PGLGEDSGKTGEPALQSEPIPADQDDGDAAADQRAMDRARGLTA
                     AQRAYGIDQGSSPQLPLGL"
     gene            199..603
                     /locus_tag="VN034_02265"
     CDS             199..603
                     /locus_tag="VN034_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HWV59449.1"
                     /translation="MKFRQNLYIDAKLSDALEALAASPGATKSGIVSAALAAYLAQRG
                     AKEIDDRLKIRLDRMSRDIHRIARDIEVLLESLSLFIRFELSVTAPLAEADHAARAVG
                     RERFEAFVAQVGRQIAGGKRSIGPLPGEEIGR"
     gene            600..1583
                     /gene="trbB"
                     /locus_tag="VN034_02270"
     CDS             600..1583
                     /gene="trbB"
                     /locus_tag="VN034_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HWV59450.1"
                     /translation="MTGNLSAGRRRAMLCTAMGPTIAAALADPRTQEIMVNPDGALRI
                     DRLGEGRIDSGVRYDPAQVERIIRLVASHARTEVHAASPIVSAELPPHGAGAGERFEG
                     VLPPVSLAPCFSIRKPAARIYTLLDYVRDGIMSADAARILSLAVAERQNILIVGGTSS
                     GKTTLANALLAEMDALDERVILIEDTRELQCPAADSVALRTRAGVVTMADLVRSTLRL
                     RPDRIIVGEVRGAEALDMLKAWNTGHPGGIATVHANSALSALYRIESLVQESVVTVPR
                     RLIAEAIDLIVFIAGRGLSRRVETLARVGGLDAQDGYVVVDHDPHPNNAGD"
     gene            1587..1922
                     /locus_tag="VN034_02275"
     CDS             1587..1922
                     /locus_tag="VN034_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HWV59451.1"
                     /translation="MRAKAVSPHILSSIALAAIGTGLCLAATAHAAGSGMPWEDPLQR
                     ILESVQGPVAKIIAAIIIITTGLTLAFGDTSGGFRRMIQIVFGVSVAFGATSFFLDFF
                     SFGGGALIA"
     gene            1919..2203
                     /locus_tag="VN034_02280"
     CDS             1919..2203
                     /locus_tag="VN034_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HWV59452.1"
                     /translation="MTGHSIHAAAPIEGFEAPVHRALAEPILLGGAPRAIAIFIGTLA
                     AALGLGLQQWIAGLVVWAVGHSVAVMAAKRDPDFAPVLLRHIKQRGHFAC"
     gene            2197..>3726
                     /locus_tag="VN034_02285"
     CDS             2197..>3726
                     /locus_tag="VN034_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041093.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HWV59453.1"
                     /translation="MLNLAEYRRRADRLADHLPWAALIAPGVVLNKDGSFSRVLAFRG
                     PDLESATEAELVSVAARANNILKRFGSGWALHIEADRREALGYPASRFPDAASWLVDE
                     ERRAAFESAEAHFESHYYFTLTFLADADQVDRAGRAFVERGGRSQGRDWRQALAAFVA
                     ETNRVLDLFSDIMPAVRALDDAGMLTYLHGTISERRHDVAVPETPLYLDGLLADTPLV
                     GGLEPMLGPLHLRTLTILGFPNLSRPGVLDALNHEGFSYRWVTRFIALDKTDATKALT
                     RLRRQWFNKRKSISALLREVMYNQPAQLLDSDADNKMLDADLALQALGGDHVAFGHLT
                     TTITVSDVSRSRVEEKLRAVERIVGGLGFTAIRESANAVEAWLGSLPAQVYANVRQPL
                     VHTLNLAHLMPLSSVWAGPARNNHLGGPPLLTAETSGSTPFRLSSHVGDVGHMMIVGP
                     TGAGKSVLLGLLALQFRRYPDAQIHIFDKGYSARAAVLAMGGAHHALGLGADGERGDG
                     GR"
BASE COUNT          562 a         1305 c         1237 g          622 t
ORIGIN      
        1 accccggcct cggcgaggat agcggcaaga ccggcgagcc ggcgttacag agcgagccca
       61 tccccgccga ccaggatgat ggtgacgccg ccgccgacca gcgcgcgatg gaccgcgcgc
      121 gaggcctcac ggcggcgcag cgcgcctatg gcatcgatca gggcagcagc ccgcaactgc
      181 cgctcggtct ttgatccgat gaagttccgg caaaacctct atatcgacgc gaagctcagc
      241 gacgcgctcg aggccctcgc ggcgagtccc ggtgcgacca agagcggcat cgtcagcgcc
      301 gcgctcgccg cctatctggc gcagcgcggg gcgaaggaaa tcgacgaccg gttgaagatc
      361 cggctcgatc gcatgtcgcg cgatatccac cgcatcgcgc gcgacatcga agtcctgctc
      421 gagagcctgt cgctgttcat ccgcttcgag ctcagcgtca ccgcgccgct cgccgaggcg
      481 gaccatgccg cgcgcgccgt cggacgcgag cgcttcgagg ctttcgtcgc gcaggtcggg
      541 cgtcagatcg caggcggcaa acgctcgatc ggccccttgc ccggcgagga gatcgggcga
      601 tgaccgggaa tctgtccgcc ggacgccgcc gcgccatgct gtgcaccgcg atggggccga
      661 cgatcgcggc ggcgctcgcc gatccacgca cccaggaaat catggtcaat cccgatggcg
      721 cgctccgcat cgaccggctc ggagaggggc gtatcgacag cggcgtgcgc tacgatccgg
      781 cgcaggtcga gcgcatcatc cgcctcgtcg cgagccacgc gcgcaccgaa gtccatgccg
      841 cctcgccgat cgtctcggcc gaactgccgc cgcatggagc gggggcgggc gagcggttcg
      901 agggtgtgct gccgccggtc agcctcgcgc cctgcttctc gatccgcaag cccgccgcgc
      961 gcatatacac tttgctcgat tatgtccggg acggcatcat gtccgccgac gctgcgcgca
     1021 tcctgtcgct tgccgtggcc gagcggcaga atatccttat cgtcggcggc acgagttcgg
     1081 gcaaaacgac gctcgccaac gccctgctcg ccgagatgga cgcgctcgac gaacgcgtga
     1141 tcctgatcga ggacacgcgc gaactccagt gcccggcggc cgacagcgtc gcgctgcgca
     1201 cgcgcgccgg ggtcgtgacg atggccgatc tggtgcgctc gacgctccgg cttcggcccg
     1261 accgcatcat cgtcggcgag gtgcgcggcg ctgaggcgct ggacatgctc aaagcctgga
     1321 acacggggca tcccggcggc atcgccaccg tgcacgcgaa ctcggcgctg tcggcgctct
     1381 acaggatcga gagcctcgtc caggaaagtg tcgtcaccgt gcccaggcgc ctgatcgccg
     1441 aagcgatcga tctcatcgtc ttcatcgcgg ggcgcgggct gtcgcgccgg gtcgagacgc
     1501 tcgcgcgcgt cggcggcctc gatgcgcagg acggttacgt cgtcgtcgac cacgaccccc
     1561 acccgaacaa cgcaggagac tgaaccatgc gtgccaaagc ggtgtccccg cacattctgt
     1621 cttcgatcgc gctcgctgcg atcggcacgg gtctttgcct cgccgccacc gcccatgcgg
     1681 cggggtcggg catgccctgg gaggacccgc tgcagcggat cctcgaatcg gtccaggggc
     1741 cggtggccaa gatcattgcc gcgatcatca ttatcacgac cggtctgacg ctggcgtttg
     1801 gcgacaccag cggcggcttc cggcgaatga tccagatcgt cttcggcgtg tcggtcgcgt
     1861 tcggggcgac gagcttcttt ctcgatttct tcagcttcgg cggcggggcg ctcatcgcat
     1921 gaccggccat tcgattcatg cggccgcgcc gatcgaaggc ttcgaggcgc cggtgcaccg
     1981 cgcgctcgcc gagccgatcc tgctcggcgg cgcgccgcgc gcaatcgcca tttttatcgg
     2041 cacgctcgcg gcggcgctgg gtctcggcct tcagcagtgg atcgccgggc tcgtcgtgtg
     2101 ggcggtcggc cacagcgtcg cagtgatggc cgcgaagcgc gatcccgatt tcgccccggt
     2161 gctcctgcgg cacatcaagc agcgggggca tttcgcatgc tgaatctcgc cgaatatcgc
     2221 cgcagggccg atcgcctcgc cgaccatctg ccatgggcgg cgttgatcgc gcccggtgtg
     2281 gtcctcaaca aggacggcag cttttcgcgt gttctcgcgt tccgcggtcc cgatctggaa
     2341 tcggcgaccg aagccgagct cgtctccgtc gcggcgcgcg cgaacaatat tctcaagcgc
     2401 ttcggttcgg gctgggcgct ccatatcgag gccgaccggc gcgaggcgct gggctatccg
     2461 gcgagccgct ttcccgacgc ggcgtcgtgg ctcgtcgacg aggagcgccg cgccgccttc
     2521 gaaagcgccg aggctcattt cgagagccat tattacttca cgctcacctt tcttgccgac
     2581 gccgaccagg tcgatcgtgc cggccgcgcc ttcgtcgagc gcggcggccg gagccagggt
     2641 cgtgactggc ggcaggcgct cgcggctttc gtcgccgaga cgaaccgcgt gctcgatctt
     2701 ttttcggata tcatgccggc ggtgcgcgcg ctcgacgatg ccgggatgct gacctatctg
     2761 cacggcacga tctcggagcg gcggcacgac gtcgcggttc ccgaaacccc gctctatctc
     2821 gacggcctgc tcgccgacac cccgctcgtc ggcgggctcg agccgatgct cggtccgctg
     2881 catctgcgca cgctgacgat cctcggtttc ccgaatctca gccggccggg ggtcctcgat
     2941 gcgctcaatc acgaaggatt ctcctatcgc tgggtgacgc ggttcatcgc gctcgacaag
     3001 acggacgcca cgaaggcgct gacccggctg cgccgccagt ggttcaacaa gcgcaagtcg
     3061 atttcggcgc tgctgcgcga ggtgatgtac aaccagcctg cgcagctcct cgacagcgac
     3121 gccgacaaca agatgctcga tgccgatctg gcgctgcagg cgcttggcgg cgatcatgtt
     3181 gctttcggcc atctgacgac gacgatcacc gtgtcggacg tgagccggtc ccgggtcgag
     3241 gaaaagctcc gcgcggtcga acggatcgtg ggcgggctcg gcttcaccgc gatccgcgaa
     3301 agcgcgaatg cggtcgaagc ctggctcggc tcacttccgg cgcaggttta cgccaatgtc
     3361 cgccagccgc tcgtccacac gctcaacctc gcgcacctga tgccgctgtc ttcagtatgg
     3421 gccggccccg cgcgcaacaa tcatctcggc ggcccgccgc tgctcaccgc ggaaaccagc
     3481 ggctcgacgc cgttccgcct ttcgagccat gtcggcgacg tcggccatat gatgatcgtc
     3541 ggaccgacag gggcgggtaa atcggtcctg ctcggcctgc tcgcgctaca gttccgccgc
     3601 tatccggatg cgcaaatcca tattttcgac aagggctatt cggcgcgcgc cgccgtgctg
     3661 gcgatgggcg gtgcgcacca cgcgctcggt ctcggcgccg acggtgaacg cggcgacggc
     3721 ggccgg
//
LOCUS       DATMSU010000084         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181877,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000084 DATMSU010000000
VERSION     DATMSU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181877"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VN034_02290"
     CDS             <1..508
                     /locus_tag="VN034_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59454.1"
                     /translation="LAPEVERAIVAPGNASFQPSPSDEEGAVDRFAEYTLALEKRDFA
                     AAYAMFRLSFQASNPRLEWEMNQRKRETLWADGQIRPLRLSWYLDPAGQPAGLYAAFD
                     FRGDRKDGTMDCGYVVVHRAQPGAGWTVVRTDTSEVPPELIEDGVPKAAVLRQLPCYL
                     GKGIATAL"
     gene            581..>2757
                     /gene="alaS"
                     /locus_tag="VN034_02295"
     CDS             581..>2757
                     /gene="alaS"
                     /locus_tag="VN034_02295"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWV59455.1"
                     /translation="MTSTNDIRRSFLDYFGQEGHHIEPSAPLVPYNDPTLMFVNAGMV
                     PFKNVFTGLEKRPYSTAVSAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KEQAIHHAWTLITKTWGLAPEKLTATVYHTDDEAFDLWKKIAGLPDERIIRIATSDNF
                     WSMGDTGPCGPCSEIFYDHGDHIWGGPPGSPEEDGDRFVEIWNLVFMQYEQMPGGERV
                     DLPRPSIDTGMGLERVAAVMQGVHDNYDTDTFQALIAASVDLTGVPAEGATQESHRVI
                     ADHLRASSFLVADGVLPSNEGRGYVLRRIMRRAMRHAHLLGAKDPLMHRLLPSLTAEM
                     GAAYPELIRAQPLIAETLEREETKFRQTLDKGLKLLDEATVGMGKGDTLAGEVAFKLY
                     DTYGFPYDLTEDALRTQDIAVDRAGFDAAMAQQKAAARAAWKGSGEKASDEIWFDIAE
                     TNGSTEFTGYSSTAGEATVIGLVKDGKPVDEAKAGDDVVVLTNQTPFYGESGGQMGDA
                     GTIATLEGVKAVVSDTGKPLGRLHTHQAKLEAGTLKVGDTVQLSVDAERRDRIRANHS
                     ATHLLHAALRNQLGGHVTQKGSLVAEDRFRFDFSHPKALSAEEIAQIESQVNAQIRGN
                     EAVSTRLMTPDDAVAAGALALFGEKYGDEVRVLSMGAADDHHYSVELCGGTHVRALGD
                     IALFKIVSESAVSSGVRRIEALTGEAARQWLNGRDEALKAAAAA"
BASE COUNT          508 a          881 c          945 g          423 t
ORIGIN      
        1 gctcgcgccc gaagtcgagc gcgcgatcgt cgcgcccgga aatgcctcct tccagccgtc
       61 gccgtccgat gaggagggcg cggtcgaccg gttcgccgaa tatacgctcg cgctcgaaaa
      121 gcgcgatttc gcagcggcct atgcgatgtt ccgcctgtcg ttccaggcat cgaacccgcg
      181 gctcgaatgg gaaatgaatc agcgcaagcg ggaaaccctg tgggccgatg ggcaaatccg
      241 cccgctgcgc ctcagctggt atctcgatcc cgcggggcag ccggcggggc tctatgccgc
      301 cttcgatttc cgcggcgacc gcaaggacgg cacgatggac tgcggctatg tcgtggtcca
      361 ccgcgcgcag ccgggcgccg gctggaccgt cgtgcgcacc gacacgtccg aagtgccgcc
      421 cgagctgatc gaggatggcg tgcccaaggc cgctgtcctg cgccagctgc cttgctatct
      481 gggcaaggga atcgcgaccg cgctctgaca gattgcgccg atgacggtca tcgacactaa
      541 tcaccttgtc gcatcgccgc aaaatgccta attcgcgcgc atgacatcga ccaacgacat
      601 ccgccgctct ttcctcgact atttcgggca ggaaggccac catatcgagc cgtcggcgcc
      661 gctggtgccg tataacgacc cgacgctgat gttcgtcaac gcgggcatgg tgccgttcaa
      721 gaatgtcttc acggggctcg agaaacgccc ctacagcacc gcggtgtcgg cgcagaaatg
      781 cgtgcgtgcg ggcggcaagc acaatgatct cgacaatgtc ggctacaccg cgcggcacca
      841 tacattcttt gaaatgctgg ggaatttctc cttcggagac tatttcaagg aacaggcgat
      901 ccaccatgcg tggacgctga tcacgaagac ttgggggctc gcgcccgaga agctgaccgc
      961 gaccgtctat cacaccgacg acgaggcgtt cgacctgtgg aagaagatcg ccggcctgcc
     1021 cgacgagcgc atcatccgca tcgccacgag cgacaatttc tggtcgatgg gcgacaccgg
     1081 gccgtgcggg ccgtgcagcg aaatcttcta tgaccatggc gaccatatct ggggcggccc
     1141 gccgggctcg cccgaagagg atggcgaccg tttcgtcgag atctggaacc tggtgttcat
     1201 gcagtatgag cagatgccgg gcggcgagcg cgtcgacctg ccgcggccga gcatcgacac
     1261 cggcatgggg ctcgagcgcg tcgccgcggt gatgcagggc gtccacgaca attatgacac
     1321 cgatacgttc caggcgctga tcgccgcgtc tgtcgacctg accggcgtgc ccgccgaagg
     1381 cgcgacgcag gagagccacc gcgtgatcgc cgatcacctg cgcgcatcga gcttcctcgt
     1441 cgccgacggc gtgctgccct cgaacgaggg ccgcggctat gtgctgcgcc ggatcatgcg
     1501 ccgcgcgatg cgccatgcgc acctgctggg ggcgaaagac ccgctgatgc accggctgct
     1561 gccgtcgctg accgccgaga tgggcgccgc ctatcccgaa ctgatccgcg cgcagccgct
     1621 gatcgccgag acgctcgaac gcgaggagac gaagttccgc cagacgctcg acaaggggct
     1681 caagctgctt gacgaggcga cggtgggcat gggtaagggc gacacgctgg ctggcgaggt
     1741 cgcgttcaag ctctacgaca cctatggctt cccctacgat ctgaccgaag acgcgctgcg
     1801 cacgcaggat atcgcggtcg accgcgcggg cttcgacgcg gcgatggcgc agcagaaggc
     1861 ggcggcgcgc gcggcgtgga agggcagcgg cgagaaggcg tcggacgaaa tctggttcga
     1921 cattgccgag acgaacggca gcaccgaatt caccggctat agctcgaccg caggcgaggc
     1981 gacggtgatc gggctcgtca aggacggcaa gcccgtcgac gaggcgaagg cgggcgacga
     2041 tgtggtcgtg ctgaccaacc agacgccctt ctatggcgag agcggcggcc agatgggcga
     2101 cgcggggacg atcgcgacgc tcgaaggcgt caaggcggtg gtgagcgata ccggcaagcc
     2161 gctcggccgc ctgcatacgc atcaggcaaa gctcgaagcg ggaacgctga aggtcggcga
     2221 tacggtgcag ctcagcgtcg acgccgaacg ccgcgaccgc attcgcgcca atcacagcgc
     2281 gacgcacttg ctgcatgcgg cactgcgcaa ccagctcggc gggcatgtga cgcagaaggg
     2341 cagcctcgtc gccgaggatc gcttccgttt cgacttctcg catcccaagg cgctgagcgc
     2401 cgaggagatc gcgcagatcg aatcgcaagt gaacgcgcag atccgcggca acgaggcggt
     2461 gtcgacgcgg ctgatgacgc ccgacgacgc cgtcgcggcg ggcgcgctgg cgctgttcgg
     2521 cgagaaatat ggcgatgaag tgcgcgtgct cagcatgggt gcggcggacg atcatcatta
     2581 ttcggtcgag ctttgcggcg ggacgcacgt ccgcgcgctg ggcgacatcg cgctgttcaa
     2641 gatcgtcagc gaaagcgcgg tttcctcggg cgtgcggcgc atcgaggcgc tgaccggcga
     2701 ggcggcgcgc cagtggctca atggacgcga cgaggcgctg aaggcggcgg cggcggc
//
LOCUS       DATMSU010000085         2158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_96647, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000085 DATMSU010000000
VERSION     DATMSU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96647"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VN034_02300"
     CDS             complement(<1..894)
                     /locus_tag="VN034_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWV59456.1"
                     /translation="MIKLLDTLHRWTGGLIGLVLALLGLSGTILLYEHLWIGVPGAGD
                     PLRGDLATVAATTERLMAMPGAQGIIYADERFGLHQLRFGGQGNEGAGAYASQSGEIA
                     ARWASQWERPELWIFDFHHHLFAGDNGEWVIGVAGLCGLFFVISGVILWWRTRRTFQL
                     RLWPKRMSRPAIVMHHRDLGVVVAPLLLISIVTGTMMIFRPFALGMVAPFGPVAETAK
                     ALDPPKYRGGPLADKPDYTAMLTEAHRRFPDAEFRILSLPRKAGDPISLRMKQPAEWL
                     PNGRTTLAFDAATGDVLGARDA"
     gene            complement(894..>2158)
                     /locus_tag="VN034_02305"
     CDS             complement(894..>2158)
                     /locus_tag="VN034_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59457.1"
                     /translation="SQIFWNRSRDTFGGEPNPIASFQDPTIAPVGTLFDQSQNRSRKI
                     GGKLSYERAVPGFEALTLIAGFDALWDTTEQRLIATDRAWVPPSEFRSFAPFGQANLK
                     LADGLVRLAGGARYENVKIKIDDYRTLAANTFVACPAPPAVPSGPCGVSTYGGVDVAG
                     GSPSFDDILLNGGVIVEPAAGIRAYASYAEGYTVPDIGRITRAVKVAGVDIDNYLDIA
                     PIISNNREIGVEVKRGPLDASATYFWSTSTKGQLLIARPDGIFDVVRQRVEIEGLEIN
                     LAVKLPIDGLTVSAGYAHITGRFDEDQDGAVDTDLDGANISPDRLNLAAAYASGPVSA
                     RIQTQFYFARSFRGPGRDARNDFEGYNLTDAFVRYQTSLGGVSLAVQNLFDKQYISYA
                     SDTQRPTDNFFNFAGRGRTFTLGWDYRF"
BASE COUNT          354 a          686 c          768 g          350 t
ORIGIN      
        1 cgcgtcgcgc gcgccgagca cgtcgcccgt cgcggcgtcg aatgccagtg tcgtgcggcc
       61 gttggggagc cattcggcgg gctgtttcat ccggaggctg atcgggtcgc ccgccttgcg
      121 cggcaggctg aggatgcgga attcggcgtc ggggaagcgg cggtgcgctt cggtgagcat
      181 cgcggtgtaa tcgggtttgt cggcgagcgg gccaccccga tatttcggcg ggtcgagcgc
      241 cttcgcggtc tcggcgacgg ggccgaaggg cgcgaccatc cccaaggcga aggggcggaa
      301 gatcatcatc gtgccggtga cgatcgagat cagcagcagc ggcgcgacga ccaccccgag
      361 gtcgcggtgg tgcatcacga tcgcggggcg gctcatccgc ttgggccaca gacgcagctg
      421 aaaggtccgc cgcgtgcgcc accacaggat gacgccgctg atcacgaaga acagcccgca
      481 caatcccgcg acgccgatca cccattcgcc attgtcgccg gcgaacaaat ggtggtggaa
      541 atcgaagatc cagagttcgg ggcgctccca ctggctggcc cagcgcgcgg cgatttcgcc
      601 cgactggctc gcataggcgc cggcgccctc attcccctgg ccgccgaagc ggagctggtg
      661 gagcccgaaa cgctcgtcgg cgtagatgat gccctgcgcg ccgggcatcg ccatcagcct
      721 ttcggtggtc gcggcgaccg tagcgaggtc gccgcgcagc ggatcgcccg cccccggcac
      781 gccgatccac agatgctcgt agagcaggat cgtgcccgac aggccgagca gcgcgagcac
      841 gaggccgatc agcccccccg tccaacggtg cagcgtgtcg agcagcttga tcatcagaag
      901 cggtaatccc agccgagcgt gaaggtgcgg ccgcggcccg cgaagttgaa gaaattgtcg
      961 gtcggccgct gggtgtcgct cgcgtagctg atatattgct tgtcgaacag attctgcacc
     1021 gcgaggctga cgccgccgag gctcgtctga tagcggacga aggcgtcggt cagattatag
     1081 ccttcgaaat cgttgcgtgc gtcgcggccg ggaccgcgga aactgcgcgc gaagtagaat
     1141 tgggtctgga tgcgcgccga taccgggccg ctggcatagg cggcggcgag gttgagccgg
     1201 tcgggcgaaa tgttcgcgcc gtcgaggtcg gtatcgaccg cgccgtcctg atcctcgtcg
     1261 aagcggccgg tgatatgcgc atagccggcc gaaacggtca gcccgtcgat cggcagcttg
     1321 accgcgaggt tgatttccag cccctcgatc tcgacgcgct ggcgcacgac gtcgaaaatg
     1381 ccgtcgggcc gcgcgatcaa cagctggccc ttggtgctcg tcgaccagaa ataggtcgcg
     1441 ctggcgtcga gcgggccgcg cttcacctcg accccgatct cgcgattgtt cgatatgatc
     1501 ggtgcaatgt cgagataatt gtcgatgtcg acgcccgcga ccttcacggc gcgggtgatg
     1561 cggccgatgt cgggaaccgt atagccctcg gcatagctcg cataggcgcg gatgccggcg
     1621 gccggctcga cgatgacgcc gccgttcagc aagatgtcgt cgaagctggg gctgccgccc
     1681 gcgacatcga cgccgccata ggtcgaaacg ccgcacgggc cgctgggcac cgcgggcggc
     1741 gcggggcagg cgacgaaggt gttcgcggcg agcgtgcgat aatcgtcgat cttgatcttc
     1801 acattctcat agcgcgcgcc gccggcaagg cggacgaggc cgtccgcgag cttgagattg
     1861 gcttggccga agggggcgaa gctgcggaac tcgctcggcg gcacccacgc gcggtcggtg
     1921 gcgatcagcc gctgttcggt cgtgtcccac agcgcgtcga agccggcgat cagcgtcagc
     1981 gcctcgaaac cggggaccgc gcgctcatag ctcagcttgc cgccgatctt gcggctgcgg
     2041 ttctgcgact ggtcgaagag cgtgccgacc ggcgcgatcg tcgggtcctg aaagctcgcg
     2101 atcggattgg gctcgccgcc gaaggtgtcg cggctgcggt tccagaagat ctggctga
//
LOCUS       DATMSU010000086         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_153414,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000086 DATMSU010000000
VERSION     DATMSU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153414"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VN034_02310"
     CDS             complement(<1..932)
                     /locus_tag="VN034_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWV59458.1"
                     /translation="MTEADRPSPETFLRQAAQEGRGRLKVFLGAAPGVGKTWEMLTDG
                     RHRREAGVDVVVGVVETHGRRETGALVEGHEVISRRHVDHQGHSLGEMDIDAILERRP
                     QLVLVDELAHTNAPGSRHPKRYQDVEELLDAGIDVYSTLNIQHVESLNDVVASFTRVR
                     VRETVPDSILENAEIEVVDIPPDELIERLREGKVYIPQEATRALTHFFSKSNLTALRE
                     LALRHAAQAVDAQMLDHVRSHALAGSFAVGERIVVAVSELPVAAELVRAGKRLADALK
                     APWSAVHIETRRSRSMSDEDRRQLADTLALASRLG"
     gene            complement(929..1519)
                     /gene="kdpC"
                     /locus_tag="VN034_02315"
     CDS             complement(929..1519)
                     /gene="kdpC"
                     /locus_tag="VN034_02315"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HWV59459.1"
                     /translation="MNKDLISALRPALVMTLLFAVLLGLAYPLALTGIGQALFPAQAN
                     GSLVRDNGQVVGSTVVGQAFTSKRYFNTRPSVAGDGYDGLASSGSNLGPTSQALADRV
                     KGDVEKMKAAEPGKAIPADLVTTSGSGLDPDISPEAAYYQVERVARARGLDPAAVRGL
                     VDRSVEGPTLGFLGEPRVNLFKLNRRLDSFGAKPAA"
     gene            complement(1530..>2380)
                     /locus_tag="VN034_02320"
     CDS             complement(1530..>2380)
                     /locus_tag="VN034_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWV59460.1"
                     /translation="LKANPGAEATAAATELRRITDEIARAGGTPLAVAQDGELLGAIF
                     LKDIVKAGIRERFGELRAMGIRTVMITGDNPLTASAIAAEAGVDDFLAQATPEDKLEL
                     IRKEQAGGRLVAMCGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDSDPTKLIE
                     VVGLGKQLLMTRGALTTFSVANDVAKYFAIIPAMFVVLYPGLGVLNIMGLTSPESAIL
                     SAIIFNALIIPLLVPLALRGVTYRPMGAGPLLARNLAIYGLGGLVAPFVGIKLIDLAV
                     GGLGLV"
BASE COUNT          389 a          791 c          799 g          401 t
ORIGIN      
        1 ccgagccgcg aggcgagcgc gagcgtatcg gcgagctgcc ggcgatcctc gtccgacatg
       61 ctcctgctgc gccgcgtctc gatatggacc gcgctccacg gcgccttgag cgcatcggcg
      121 agccttttgc ccgcgcgcac aagttcggcg gcgacgggga gttcgctcac cgcgacgacg
      181 atgcgctcgc cgaccgcgaa gctgcccgcc agcgcgtggc tgcgcacatg atcgagcatc
      241 tgcgcgtcga ccgcctgcgc cgcatggcgg agcgcaagct cgcgcaaggc ggtcaggttc
      301 gacttggaga agaaatgggt gagcgcgcgc gtcgcttcct gcgggatata gactttcccc
      361 tcgcgcagcc gttcgatcag ctcgtcgggc gggatgtcga caacctcgat ctcggcattt
      421 tcgaggatcg agtcgggcac ggtttcgcgg acgcggacgc gggtgaaaga ggcgacgacg
      481 tcgttgaggc tttcgacgtg ctggatgttg agcgtcgaat agacgtcgat gcccgcgtcg
      541 agcagttcct cgacatcctg atagcgtttg ggatgccggc ttcccggcgc gttggtgtgc
      601 gctagttcgt cgacgaggac gagctgcggc cgccgttcga ggatcgcgtc gatatccatt
      661 tcgccgaggc tgtgaccctg atggtcgaca tggcggcgcg aaatgacctc atgtccttcg
      721 acgagcgccc cggtctcgcg ccggccatgc gtttcgacca cgccgacgac gacatcgacc
      781 ccggcctcgc ggcgatggcg accgtcggtg agcatttccc acgtctttcc gacccccggc
      841 gccgcgccca gaaagacctt gagacggccg cgaccttcct gcgccgcctg gcgcagaaag
      901 gtctcgggcg atggtcggtc ggcttcggtc acgcggcggg tttagctcca aaactatcca
      961 gacgtcgatt gagcttgaac agattgacac gcggctcgcc aagaaagccc agcgtcgggc
     1021 cttcgacgct ccgatcgacg agcccccgca ccgccgccgg atcgagcccg cgcgcccggg
     1081 cgacgcgctc gacctgataa taggccgcct cgggcgaaat gtcagggtcg aggcccgatc
     1141 ccgaggtcgt gacgagatcg gcggggatgg ccttgccggg ctccgccgcc ttcatctttt
     1201 cgacatcgcc cttcacacgg tcggcgagcg cctgcgacgt cgggccgagg ttcgatcccg
     1261 acgatgcgag cccgtcatat ccgtcgcccg ccaccgacgg ccgcgtgttg aaatagcgtt
     1321 tcgacgtgaa ggcttgaccg acgaccgtcg agccgacgac ctggccgttg tcgcggacga
     1381 ggctgccatt ggcttgggcc gggaacaggg cctgcccgat gccggtgagc gcgagcgggt
     1441 aggccagccc gagcaggacc gcgaagagta aggtcatgac gagtgccgga cgcagcgcac
     1501 tgataagatc cttgttcata atggcctcct tagaccagac ccaggccgcc gacggcgagg
     1561 tcaatgagtt tgatgccgac gaagggcgcg acgaggccgc caagtccgta aatggcaaga
     1621 ttgcgcgcca gcagcggccc cgcccccatc ggacgatagg tcacgcccct cagcgcaagc
     1681 ggcacgagca gggggatgat cagcgcgttg aagatgatcg ccgagaggat cgcgctctcc
     1741 gggctggtca gccccatgat gttgagcacg ccgagccccg gatagagcac gacgaacatc
     1801 gccgggatga tcgcaaaata tttggcgacg tcattggcga ccgaaaaggt ggtgagcgca
     1861 ccgcgcgtca tcaggagctg cttgccgagc ccgacgacct cgatcagctt cgtcggatcg
     1921 ctgtcgaggt cgaccatatt gcccgcttcg cgcgctgcct gcgtccccgt gttcatcgcg
     1981 acgccgacgt cggcctgcgc gagcgcgggc gcgtcgttgg tgccgtcgcc gcacatcgcg
     2041 acgagccgcc cgcccgcttg ctccttgcgg atcagctcga gcttgtcctc gggcgtcgcc
     2101 tgcgcgagga aatcgtccac cccggcttcg gcggcgatcg ccgaagcggt gagcgggttg
     2161 tcgccggtga tcatcaccgt gcggattccc atcgcgcgca attcgccgaa acgttcgcgg
     2221 atccccgcct tgacgatgtc cttcaaaaag atcgcgccga gcagttcgcc gtcctgcgcc
     2281 accgcgagcg gggtgccgcc ggcgcgggcg atctcgtcgg tgatacggcg gagttcggtc
     2341 gcggcggcgg tcgcctccgc acccggattg gccttcagca
//
LOCUS       DATMSU010000087         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_162641,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000087 DATMSU010000000
VERSION     DATMSU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162641"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VN034_02325"
     CDS             complement(<1..1384)
                     /locus_tag="VN034_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV59461.1"
                     /translation="MTKNLVRRAATALSPLVLLIASTAPAHARETPAKAAAPATVNPQ
                     DAAAKTWNFAASDIPVDPNIVFGVLPNGMKYALLKNSTPKDSVVLRMRFDVGSFAEAD
                     DQRGLAHFLEHMAFNGSTKVPEGEMIKLLERKGLAFGADTNASTGFDETVYKLDLPNA
                     SDDLIDTGLMLMRETASELTIDPAAVDRERGIILSERRARDTYQLRSLIDQLDFQMRG
                     MTVASRIPVGTEEVIKTAPAARIRDLYDRYYRPERATLVMVGDFDPAAVEAKIKARFS
                     DWKGRGPAGADPDIGDVDYDRAAAADDFVDPAIQDSVTIARFKPWLDEPDTKAKRARK
                     LAEDVGEAIVSRRLAKIALTEDSPILTGYFSDASGWKKFDQVTVGAVAKEGAWKEALA
                     LVEQEQRRAIEHGFTQAEVDEQLAIRRTAFRNAVAGVTTRRSETLADALVTAAEGDFV
                     FTRPETSQALF"
     gene            complement(1428..2573)
                     /locus_tag="VN034_02330"
     CDS             complement(1428..2573)
                     /locus_tag="VN034_02330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWV59462.1"
                     /translation="MTGVYHTLQARFFPPIPDAIRDDVALLRANRVETLTPMLFLMLA
                     ATTPTAIYGGVETVHPFIRIGFPVILAIVGVLGFLTLRHNRGRRMSPRRARRIIREAS
                     WVSGITGAMCSLWTVTNWLAAPPETHSYYAMIMAMGSLATAYCLSSIRFATFLNLGVG
                     LAPVSALMLTSGNPPEIAAGTSLVIATLFLLRMVVQQHGQVIDLLELQRQMRELAHTD
                     PLTGLANRREFDLRLDEEIAKADGAGRFAIALLDLNGFKPINDRHGHAIGDGVLCELA
                     ERLRRACGHHAIVARQGGDEFAILVPAGSPLLGTALADHILAALAAPYRIDGRSIGVG
                     AGVGTAYWPEHGDSARQLLEVADRALYAAKAANRTLTSSVESVGRVA"
BASE COUNT          451 a          913 c         1005 g          468 t
ORIGIN      
        1 cgaacagcgc ctgcgaggtt tcggggcgcg tgaagacgaa atcgccctcg gccgccgtga
       61 ccagcgcgtc ggcaagcgtc tcgctgcgcc gcgtcgtcac gcccgcaacg gcattgcgga
      121 aagcggtgcg gcggatcgcg agctgctcgt cgacctcggc ctgggtgaag ccatgttcga
      181 tcgcgcggcg ctgttcctgc tcgaccagcg cgagcgcctc tttccacgcg ccttccttgg
      241 cgaccgcgcc gacggtgacc tggtcgaact tcttccagcc cgacgcatcg ctgaaatagc
      301 cggtgaggat cggcgaatcc tcggtgagcg cgatcttcgc gagccggcgg ctgacgatcg
      361 cctcgccgac atcctcggcg agcttgcgcg cgcgtttcgc cttggtgtcg ggctcgtcga
      421 gccacggctt gaagcgcgcg atcgtcaccg aatcctggat cgcggggtcg acgaaatcgt
      481 ccgccgccgc cgcgcgatca taatcgacat cgccgatgtc gggatcggcg cctgcgggac
      541 cgcgcccttt ccagtccgaa aagcgcgcct tgatcttcgc ctcgaccgcc gcgggatcga
      601 aatcgccgac catcaccagc gtcgcgcgct cggggcggta atagcggtcg tagagatcgc
      661 ggatgcgcgc ggcgggcgcc gtcttgatca cctcttcggt gccgacgggg atgcggcttg
      721 ccaccgtcat cccccgcatc tggaagtcga gctggtcgat caggctgcgc agctgatagg
      781 tgtcgcgcgc gcgccgttcg gagaggatga tgccgcgttc gcggtcgacc gcggcgggat
      841 cgatcgtcag ttcgctcgcg gtctcgcgca tcagcatcag cccggtgtcg atcaggtcgt
      901 cggaggcgtt cggcaggtcg agcttgtaga ccgtctcgtc gaagccggtc gaggcgttgg
      961 tgtcggcgcc gaaggcgagc cctttgcgtt cgagcagctt gatcatctcg ccctcgggca
     1021 ccttggtcga gccgttgaac gccatatgtt cgaggaaatg ggcgaggccg cgctggtcgt
     1081 cggcttcggc gaagctgccg acgtcgaagc gcatgcggag cacgacgctg tccttgggcg
     1141 tgctgttctt gagcagcgca tatttcattc cgttcgggag cacgccgaag acgatgttcg
     1201 gatcgaccgg aatgtcgcta gccgcgaaat tccatgtctt tgccgccgcg tcctgcggat
     1261 tgaccgtcgc gggcgccgcc gccttcgcgg gggtttccct ggcatgggcc ggggcggtgc
     1321 tggcgatgag aaggacgagg ggcgacagcg cggttgcggc gcgccgaacg aggtttttgg
     1381 tcatggccct cgatgtggcg accgccccgc gggcggtcaa gccatcgtca tgcgacccgg
     1441 ccgacgcttt cgacagacga cgtcagggtg cgattggcgg cctttgcggc atagagcgcg
     1501 cggtcggcga cttcgagcaa ttggcgggcg ctgtcgccat gttcaggcca ataggcggtg
     1561 ccgacccccg cgccgacgcc gatcgagcgc ccgtcgatgc gatacggcgc ggcgagcgcg
     1621 gcgaggatat ggtcggcgag cgcggtgccc agcagcggcg agccggcggg cacgaggatg
     1681 gcgaattcgt cgccgccctg gcgcgcgacg atggcatgat ggccgcaggc gcggcgcagc
     1741 cgctcggcga gctcgcacaa gacgccgtcg ccgatcgcgt ggccgtgccg gtcgttgatc
     1801 ggcttgaacc cgttgaggtc gagcagcgcg atcgcgaagc ggccggcgcc gtcggccttg
     1861 gcgatctcct cgtcgagcct gaggtcgaat tcgcggcggt tggcgaggcc ggtgagcgga
     1921 tcggtatgcg ccagctcgcg catctggcgc tgcagttcga gcaggtcgat cacctgtcca
     1981 tgctgctgga cgaccatgcg cagcaggaac aaggtcgcga tgacgaggct ggtgcccgcc
     2041 gcgatttcgg gcggattgcc cgaggtcagc atcagcgccg acaccggcgc gagcccgacg
     2101 ccgaggttga ggaaggtcgc gaagcggatc gacgacaggc aataagcggt cgcgagcgac
     2161 cccatcgcca tgatcatcgc ataataactg tgcgtttcgg gcggcgcggc gagccagttg
     2221 gtcaccgtcc acaggctgca catcgcgccg gtgatccccg acacccagct cgcctcgcgg
     2281 atgatgcgcc gcgcgcggcg cgggctcatc cggcggccgc gattgtggcg cagggtcaga
     2341 aagccgagca cgccgacgat cgcgaggatg accggaaaac cgatgcggat gaacggatgc
     2401 accgtttcga ccccgccata gatggcggtg ggcgtcgtcg ccgcgagcat caggaacagc
     2461 atcggggtca gcgtctcgac gcgattggcg cgcagcaacg cgacgtcgtc ccgaatcgca
     2521 tccggaatcg gcggaaagaa gcgcgcctgc agcgtgtgat agacccccgt catgggggcg
     2581 ggatagcaag gccgggtaaa gcaggggtta aggaatgaaa gcttggctta tcacggcgct
     2641 aagggcgcat cgggggcgga tggcttgcga aatattaacc atgttcgccg atacgcgcgg
     2701 cctgatgttt cgcgcccgac aggagccacg ccatgtctta tccgacgccg cagcttgacc
     2761 catccaccgc cgcgccgacg gcgccgatgc cgtccgccca cccgacgctg ccgcaggacg
     2821 atacgcccgc cgcgcag
//
LOCUS       DATMSU010000088         4128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_87173, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000088 DATMSU010000000
VERSION     DATMSU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87173"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VN034_02335"
     CDS             <1..703
                     /locus_tag="VN034_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59463.1"
                     /translation="GLADRGYDIADPAFHPFDWGSMTAMVALLEMRLDRLASDADAGD
                     ASDPCAAINRDACERAREIIGIVHDSAARDTFSPGDATAVMAALHAIRWAKAARTVDP
                     ETGAERIETITLPLTTHRPGAVLWVQDYQTILFDRAKHAWCANPDNLSAPARAFYEPW
                     WREMAAFEMAAVGGEPLRPLSGFDGDRDMVLLELPSSGLMSRKFGDDADLVVTIGRAE
                     LAIGDFSKARAQLSS"
     gene            809..1531
                     /gene="phbB"
                     /locus_tag="VN034_02340"
     CDS             809..1531
                     /gene="phbB"
                     /locus_tag="VN034_02340"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HWV59464.1"
                     /translation="MARIAIVTGGSRGIGEAISLKLKEQGVTVVANYAGNDEKAREFT
                     DRTGIAARKWDVGDHQACLDNCAAIAEEFGAIDIVVNNAGITRDGTLARMSYDDWDDV
                     MRVNLGGCFNMAKATFGGMVERGWGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGAKKGVTVNAIAPGYIDTDMVAAVPAPVLEKIVAKIPVGRLGQADEIARGVAFL
                     CSEDAGFVTGSTMSINGGQHMY"
     gene            complement(1818..2588)
                     /locus_tag="VN034_02345"
     CDS             complement(1818..2588)
                     /locus_tag="VN034_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HWV59465.1"
                     /translation="MLSGFQALTEKEKESLRLLVDGHDAKSMARHLGLSVHTINERLR
                     DARRKMAVSSSREAARQLREIEHRTPEYSGDRLLGDAAALHDTEELRTPAGTRRISRR
                     VGWIAGGIFMSISLALFALASLHGTAPTPESPPATLPTMTLSAPASETAAVQAARQWL
                     ALVDKGDWSGSWNATGAAFKSLNSVERWTEVSQSVRPPLGAVISRELVSEQEVPAPPY
                     GYQMVKFRTDYANKAGAIETLSLVREGGSWRVVGVTVE"
     gene            2729..3271
                     /locus_tag="VN034_02350"
     CDS             2729..3271
                     /locus_tag="VN034_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59466.1"
                     /translation="MTDPALSAAQQDLARAYAGGAPGVFVSGVVWLVAGAVWQLHGTM
                     VAFAALFIGGMAIHPLGVLIERIAFRAPKATIGKPLETLALEATIPLFVGVMIAWVLL
                     VRAPDLAIPAFAAIIGGRYFAFRTLYGDIAYWALGAAILLVAGLALFGIGLPVNAAIL
                     VGVIELVAAALILRRWRARR"
     gene            3268..3516
                     /locus_tag="VN034_02355"
     CDS             3268..3516
                     /locus_tag="VN034_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWV59467.1"
                     /translation="MSAPLHPPVKRSVTIAGHPTSISLEPVFWDALEAEAARLALPVN
                     ALVARIDVERMEAGDPPNLTSAIRQWLWRERIESDLRP"
     gene            3534..3728
                     /locus_tag="VN034_02360"
     CDS             3534..3728
                     /locus_tag="VN034_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59468.1"
                     /translation="MKSTKIIAPILAGGATIGMLVDIGLQISSGSTDNLLMNIAGWLV
                     FIAFIAFVWHATILHIEHGD"
     gene            complement(3725..>4128)
                     /locus_tag="VN034_02365"
     CDS             complement(3725..>4128)
                     /locus_tag="VN034_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59469.1"
                     /translation="GAPVPADALGQRRLVQPLMPGILTEGEFSLFFFAGKFSHAIVKR
                     PASGDFRVQEQFGGRETVWDASDAAQALAAQALAAAPAPPIYARVDMVGDAAGTLHIM
                     ELELIEPSLFLHHAPDKGAAFGSAVAGVLFR"
BASE COUNT          704 a         1395 c         1332 g          697 t
ORIGIN      
        1 cgggcttgcc gatcgcggct atgacatcgc cgaccccgcc ttccacccct tcgactgggg
       61 cagcatgacc gcgatggtcg cgctgctcga aatgcggctc gaccggctcg caagcgatgc
      121 ggacgcgggc gacgcatcgg acccatgcgc cgcgatcaac cgcgacgcgt gcgaacgcgc
      181 gcgcgaaatc atcggaatcg tccacgacag cgcggcgcgc gacacctttt cgcccggcga
      241 tgcgaccgcg gtgatggcgg cgctgcatgc aatccgctgg gcgaaggcgg ctcgcaccgt
      301 cgatcccgaa accggcgccg agcggatcga gacgatcacg ctgccgctga cgacgcatcg
      361 cccgggcgcc gtcctgtggg tgcaggatta tcagacgatc ctgttcgacc gcgcgaagca
      421 cgcctggtgc gccaatcccg acaatctgtc ggccccggcg cgcgcctttt acgagccctg
      481 gtggcgcgaa atggcggcgt tcgagatggc ggcggtgggc ggcgaaccgc ttcgcccgct
      541 gtccggtttc gacggcgacc gcgacatggt cctgctcgaa ctgccgagca gcggactgat
      601 gagccggaaa ttcggcgacg acgccgatct ggtggtgacg atcggccgcg ccgagcttgc
      661 gatcggggat ttttcgaagg cgcgcgcgca acttagcagc tagagcctgc ctatctgaat
      721 agcttttcga ttgacgttcc ggtcaacttg cgggcccccg ccccgcggcc taatcatgat
      781 gcgataacgc agtgggagag cttgatttat ggcacgcatc gcaatcgtca ccggcggcag
      841 ccgcggcatc ggggaagcga tttcgctgaa actgaaggaa cagggcgtca ccgtcgtcgc
      901 caactatgcc ggcaatgacg aaaaggcgcg cgaattcacc gaccggaccg ggatcgccgc
      961 gcgcaaatgg gacgtcggcg accatcaggc gtgcctcgac aattgcgctg ccatcgccga
     1021 ggaattcggt gcgatcgaca tcgtcgtcaa caacgccggc atcacccgcg acggcacgct
     1081 cgcgcgcatg agctatgacg attgggacga cgtgatgcgc gtcaacctcg gcggctgctt
     1141 caacatggcg aaggcgacct tcggcggcat ggtcgaacgc ggctggggcc gcatcgtcaa
     1201 catcggctcg atcaacgggc aggcgggcca gtatggccag gtcaattatg ctgccgccaa
     1261 atcgggcatc cacggcttca ccaaggcgct ggcgcaggaa ggcgcgaaga agggcgtcac
     1321 cgtcaacgcg atcgcgccgg gctatatcga caccgacatg gtcgccgcgg tgccggcgcc
     1381 ggtgctcgaa aagatcgttg cgaaaatccc ggtcggacgg ctgggccagg ccgacgagat
     1441 cgcgcgcggc gtagcgttcc tgtgcagcga ggatgcgggc ttcgtgaccg gatcgacgat
     1501 gtcgatcaat ggcgggcagc atatgtactg acgggccgag ggcgccaacg atggcgctcg
     1561 cgccagcgaa cggcgagttg gcaaccggtg gcgcagccac ggcccggcac ggccagcggg
     1621 gcggcgatct taaagatcgc cgaacccgtc tgacgacagg cggcggcttt gccgtcgccg
     1681 cctacactcc acccaatggc ctatcgcgaa gtcgagatac ctccccgacc agcagttgcg
     1741 tggatcggtg tctcgacttc gctcgacacg aacggaaaga gaggacggtt cggatttcca
     1801 aaccgccccc gcagccctca ctccaccgtc acccccacca cgcgccagct tccgccctcg
     1861 cgcaccagcg acagcgtctc gatcgctccc gccttgttcg catagtcggt gcggaacttg
     1921 accatctgat agccataggg gggcgcgggc acctcttgct cgctgaccag ctcgcgcgag
     1981 atgaccgcgc cgagcggcgg gcgaaccgat tgcgacactt cggtccatcg ttcgacgctg
     2041 ttgagcgact tgaacgctgc accggtcgcg ttccagctcc cgctccagtc gcccttgtcg
     2101 acgagcgcca gccactgccg cgccgcctgc accgcagcgg tttcggacgc gggcgccgag
     2161 agtgtcatgg tcggcagcgt ggcaggtggg gactccggtg tcggcgcggt tccgtgcagc
     2221 gacgcgagag caaataaggc aaggctgatc gacatgaata ttcctccggc tatccagccg
     2281 acgcggcgcg atatgcggcg cgttccggcc ggtgtccgaa gctcttccgt gtcgtggagg
     2341 gcggccgcat cccccaaaag cctgtccccc gaatattcgg gggtccggtg ctcgatctca
     2401 cgcagctgcc gcgcggcctc gcgactgctc gacaccgcca tcttgcgccg cgcgtcgcgc
     2461 agcctttcgt tgatcgtatg gaccgatagc ccgagatgcc gcgccatcga tttcgcatcg
     2521 tggccgtcga cgagcaggcg caggctttcc ttttcctttt cggtgagcgc ctgaaaccct
     2581 gatagcatgg cctgcctgca tttgtgcctg atcggtcggc catatttacg aagcgacccg
     2641 gcggcgatct accccaaata aatcgtacct tcggtcgcgc ccgccgcgac ggccttgctg
     2701 ccgcgaatca actcgctaca cccgaaacat gaccgacccc gcgctttccg ccgcccagca
     2761 agacctcgcc cgcgcctatg ccggcggcgc gcccggcgtc ttcgtctcgg gcgtcgtctg
     2821 gctggtcgcc ggcgcggtgt ggcagttgca cggcaccatg gtcgcctttg ccgccttgtt
     2881 catcggcggc atggcgatcc acccgctcgg cgtcctgatc gagcgcatcg cctttcgcgc
     2941 ccccaaggcg acgatcggca agccgctcga aacgctcgct ctcgaagcga cgataccact
     3001 gttcgtcggc gtgatgatcg cgtgggtgct gctggtgcgc gcgcccgacc tcgcgatccc
     3061 ggcgttcgcc gcgatcatcg gcggccgcta tttcgccttc cgcaccctct atggcgacat
     3121 cgcctattgg gcgctgggcg ccgccatcct gctggtcgcg gggctggcat tgttcggcat
     3181 cggccttccc gtcaatgccg cgattctcgt cggcgtcatc gaactcgtag ccgccgccct
     3241 gatcctccgt cgctggcgcg cgcgccggtg agcgcgccgc tccatccccc ggtcaaacgt
     3301 tcggtgacga tcgccggtca tccgacgtcg atcagcctcg agcccgtctt ttgggacgca
     3361 ctcgaagccg aagcggcgcg actggcgctg ccggtgaacg cgctcgtcgc gcggatcgat
     3421 gtcgaacgga tggaggcggg cgatccgccg aacctgacct cggcgatccg gcagtggctg
     3481 tggcgggagc ggatcgaatc ggacctcagg ccatgacgcc caaggaacgc gccttgaaga
     3541 gcacgaagat catcgcgccg atccttgccg gcggcgcgac aatcggaatg ctcgtggaca
     3601 tcggattgca gatatcgtcg ggatcaaccg acaatctgct gatgaatatc gccggatggc
     3661 tcgtttttat cgcgttcatc gccttcgtct ggcacgcgac aatcctgcac atcgagcatg
     3721 gagattagcg gaacaaaact cccgccaccg ccgatccgaa cgccgcgccc ttgtcgggcg
     3781 cgtgatgcag gaagagcgac ggttcgatca gttcgagttc catgatgtgg agcgtgcccg
     3841 cggcatcgcc gaccatatcg acgcgcgcat agatcggtgg cgccggagcg gccgccaacg
     3901 cctgcgcagc aagagcctgc gccgcatcgc tcgcatccca caccgtctcg cgtccaccga
     3961 actgttcctg cacgcgaaaa tcgcccgatg cggggcgctt gacgatcgcg tggctgaact
     4021 tgccggcgaa gaagaagagc gaaaattcgc cttcggtaag gatgccgggc atcagcggct
     4081 ggacgaggcg gcgctgccca agcgcgtcgg cggggaccgg tgcgccgg
//
LOCUS       DATMSU010000089         6225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_181978,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000089 DATMSU010000000
VERSION     DATMSU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181978"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VN034_02370"
     CDS             <1..982
                     /locus_tag="VN034_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWV59470.1"
                     /translation="IDGLVSLCGARTDGKRVRLGLRETTVQAFPIGIDVEGFRRLAAA
                     SARQATTPLRATRESLGQRKLVIGVDRLDYSKGIVQRLEAFGEFLRDHPEERGRVSLL
                     QIAPPSRSDVPEYADLDRQSDEVAGRLNAQLGEFDWTPIRVVKKAYSRKALAGFYRRA
                     QVGLVTPMRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAQQMSEALIVNPFDSHEVA
                     EAIRTALAMPKTERIRRFEKLYAIIAATDIDWWTRQYLLALSGESGAAEALAVGKERK
                     EPRAGARRTGRGETSAASSAAPEAGVRKMSAGSRRKRSAAEASHLVLVRP"
     gene            1218..3431
                     /gene="ptsP"
                     /locus_tag="VN034_02375"
     CDS             1218..3431
                     /gene="ptsP"
                     /locus_tag="VN034_02375"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWV59471.1"
                     /translation="MAASISPQERLDRLVVLIAGNLVAEVCSVYVLREDGSLELYATE
                     GLNREAVHLTTMRAGEGLVGLIASEAEPLALSDAQAHPSFSYRPETGEEVYRSFLGVP
                     ILRGGAVMGVLVIQNRASRLYSEDEIETLQTTAMLMAEMIAAGGLKSLAAPGASIGLD
                     RPIHGVGVALADGVGLGHVVLHEPRVAITNLIAENPAAEVGRLEAAIGEMRASIDELV
                     ERGDVAHTGEHRDVLETFRMFAHDQGWLRRMREVVLTGLTAEAAVERVQSDTRAKMLR
                     QTDPYLRERLHDLDDLANRLLRTLVGKANGVARENLPENAILVARSMGPAALLDYDRA
                     HLRGLVLEEGGPTSHIAIVARALGIPAVGEVVNATALVEAGDAIIVDGQAGEVQIRPQ
                     PDVENAYKDKARLRARKQKQYQKLKELPAVTRDGVDITLQINAGLIVDMPNIEATAAS
                     GIGLFRTELQFMVAQHMPTTAEQQALYAAVLEAAAELPVTFRTLDIGGDKVLPYMERV
                     EEENPALGWRAIRIGLDKPRLLRAQVRAMLRAGAGRDLKIMLPMVATTEEFRRARMII
                     RREQAMLEKQGVAPPRDLRVGVMVEVPSLLFELREIAREADFLSIGTNDLMQFLFAAD
                     RENKRVADRFDPLGVGALRALRRIIEAAAETGCPVTVCGEMGGKPLETMALIGLGYRG
                     FSMSAASIGPVKAMLRALDAGKLRERMDWMLASPEGATNLRPQLAAFATEFKVPV"
     gene            3488..4567
                     /gene="prfA"
                     /locus_tag="VN034_02380"
     CDS             3488..4567
                     /gene="prfA"
                     /locus_tag="VN034_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWV59472.1"
                     /translation="MSLQLPQDRLDVIVARHAAATDEINHATEAARAVELAKELAELD
                     PVVAAIASWRQAQISLDEAEALIADPATDSEFRDLAYEERDIARAEIEARAREILVAL
                     LPKDAADERGVILEIRAGTGGDEAALFAGDLLRMYQRHAAQKGWSLDVLSESEGTAGG
                     YKEVIAEINGRGVYARLKFESGVHRVQRVPDTEASGRIHTSAATVATLPLATDVDVTL
                     NEADIRTDTLRSGGAGGQHVNKTESAVRLTHIPTNTVVLVQDERSQHKNKARAYELLR
                     AKLFDMERLRIDAERSADRRSQVGSGDRSERIRTYNFPQGRVTDHRINLTLHRLPEII
                     EGAMDELIDALIAEDQAQRLAGLGG"
     gene            4564..5388
                     /gene="prmC"
                     /locus_tag="VN034_02385"
     CDS             4564..5388
                     /gene="prmC"
                     /locus_tag="VN034_02385"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWV59473.1"
                     /translation="MSEVSAALREAAAQLAAVSDTARLDAELLMAHALGVERPALLLD
                     ASRFALPARFADFVARRMRSEPVAYILGYRDFWTVRIGVGPGVLIPRPDSETLIEAAQ
                     AHFGTGGPTRILDLGTGPGTLLYAALAEWPAATGLGVDASDIALDYANRNALALGLER
                     QVELMQGDWARGVAGQFDLILCNPPYIADSEQLMPDVADHEPAGALFAGADGLDDYRR
                     IIPELPRLMSPRGVAILEIGATQHMLVRQLAEAAGFFVRCRQDLGGRDRALLLTRT"
     gene            5663..>6225
                     /locus_tag="VN034_02390"
     CDS             5663..>6225
                     /locus_tag="VN034_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HWV59474.1"
                     /translation="MNNRQSGRRRGRNNNSNNNNRSQSGGRGGVDQANRIDSRARGNG
                     AQMIEKYRNLARDAQLAGDRVQTEYYLQFADHYFRVVSDFRARQEEKNAQNGQDRGHD
                     RSREIRGVEDFDGHDDSDIDIDTDRDDSDNGDDRGERNDRGNDRNERGDRSQRDNRGN
                     RDDDSRGNREPRGQRDDDNRGNRQPART"
BASE COUNT         1037 a         2079 c         2116 g          993 t
ORIGIN      
        1 gatcgacgga ctcgtctccc tttgcggtgc ccgcaccgat ggaaagcgcg tgcggctggg
       61 gctgcgcgaa accaccgtgc aggcctttcc gatagggatc gacgtcgagg gcttccgccg
      121 gctcgccgcc gcctccgccc gccaggcgac gacgccgctg cgcgccacgc gtgaatccct
      181 cggtcagcgc aagctcgtca tcggggtcga ccggctcgac tactccaagg gcatcgtcca
      241 gcggctggag gcgttcggcg agttcctgcg cgaccatccg gaggagcgcg ggcgggtcag
      301 cctgctgcag attgccccac cctcgcgcag cgacgtgccc gaatatgcgg atctggaccg
      361 ccagtccgac gaagttgccg gccgtctcaa cgcccagctc ggcgaattcg actggacgcc
      421 gatccgtgtg gtcaagaagg cctattcgcg caaggcgctg gccggcttct accgccgcgc
      481 ccaggtcggc ctggtgacgc cgatgcggga cggcatgaat ctcgtcgcca aggaatatat
      541 cgcggcgcag gacccggccg atccgggcgt gctcgtgctc tcgcgcttcg ccggcgcggc
      601 ccagcagatg agcgaggcgc tgatcgtcaa tcccttcgat tcccacgagg tggcggaagc
      661 gatccgcacc gcgctcgcca tgccgaagac cgagcgcatc cgccgcttcg agaagctcta
      721 tgccatcatc gctgctaccg atatcgactg gtggacgcgg caatacctgc tggccctgtc
      781 cggtgagtcc ggggcggccg aggcattggc cgtgggtaaa gagcgcaagg aaccacgtgc
      841 cggcgcgcgg cggacgggcc ggggcgagac atcggccgcg tcatcggctg ccccggaagc
      901 cggtgtccgc aagatgtcgg caggatcgcg ccggaagcgg agtgccgccg aggcctctca
      961 tctcgttttg gtgcggccct gagacttcgg cctcgagccc aaaaacgacg gaaatcttgg
     1021 ggtgatgcta cggacgctgt tcgcggccct tgccgaataa ggtctttggt cgctagaagg
     1081 cagcatgacc ggagtgggcg actcaggtct ggacaggcat cgtatcgtcg atggctgagg
     1141 aggcgggcgg agggctgaga tcgatgcgag gcgctctcgg aggaccgggc gttctgttgc
     1201 gccgtctccg cgaggtcatg gcggcatcga tcagcccgca ggagcggctg gaccgtctcg
     1261 tcgtcctgat cgccggcaat ctcgtcgccg aagtctgctc cgtctacgtc ctgcgcgagg
     1321 acggctcgct tgaactctac gcgaccgaag gcctcaaccg cgaggcggtc cacctcacca
     1381 ccatgcgggc gggcgagggc ctcgtcggcc tgatcgcgag cgaggccgag ccgctggcgc
     1441 tgtcggacgc gcaggctcac ccttccttct cctaccgtcc ggagacgggc gaagaggtct
     1501 accgctcctt cctcggcgtg ccgattctgc gcggcggcgc cgtgatgggc gtgctcgtca
     1561 tccagaatcg ggcctcgcgg ctctattccg aggacgagat cgagaccctt cagaccaccg
     1621 ccatgctgat ggcggagatg attgcggccg gcggcctcaa gtcgctggcg gcgcccggcg
     1681 cctcgattgg cctcgatcgt ccgatccatg gcgtcggcgt ggcgctggcc gatggcgtcg
     1741 gcctcggcca tgtcgtgctg catgagccgc gcgtcgcgat caccaatctc atcgccgaga
     1801 atccggctgc cgaagtcggc cggctcgaag cggcgatcgg agagatgcga gcctcgatcg
     1861 acgaactggt cgagcgcggc gacgtcgccc ataccggcga gcatcgcgac gttctcgaga
     1921 ccttccgcat gttcgcccat gaccagggct ggctgcgccg catgcgcgag gtcgtgctga
     1981 ccggactcac cgccgaggcg gcggtcgagc gcgtccagtc cgacacccgc gccaagatgc
     2041 tgcgccagac cgacccctat ctgcgcgagc ggctgcacga tctcgacgat ctcgccaacc
     2101 ggctgctgcg cacgctggtc ggcaaggcga acggcgtcgc ccgcgagaac ctgccggaga
     2161 atgccatcct cgtcgcccgc tcgatgggcc cggcggcact gctggactac gaccgtgctc
     2221 atctgcgcgg gctcgtgctg gaggagggcg ggccgaccag ccatatcgcg atcgtcgccc
     2281 gcgcgctcgg catccccgcc gtcggcgagg tcgtcaacgc gaccgcgctg gtcgaggcgg
     2341 gggatgccat catcgtcgat ggtcaggcgg gcgaggtgca gatccgcccg cagcccgacg
     2401 tcgagaacgc ctacaaggac aaggcgcggc tgcgcgcccg caagcagaag cagtaccaga
     2461 agctcaagga gcttccggcc gtcacccgcg acggggtcga catcaccctg cagatcaatg
     2521 ccggcctgat cgtcgacatg ccgaacatcg aggcgacggc cgcctccggc atcggcctgt
     2581 tccgcaccga attgcagttc atggtggcgc agcacatgcc gaccaccgcc gagcagcagg
     2641 cgctctatgc ggccgtgctg gaggctgcgg cggaattgcc ggtcaccttc cgcaccctcg
     2701 atatcggcgg cgacaaggtg ctgccctata tggagcgggt cgaggaggag aacccggcgc
     2761 tcggctggcg cgcgatccgc atcggcctcg acaagccgcg gctgctccgg gcgcaggtgc
     2821 gcgcgatgct gcgggccgga gcggggcggg acctcaagat catgctgccg atggtcgcga
     2881 ccaccgagga gttccgccgc gcccgcatga tcatccgtcg cgagcaggcc atgctggaga
     2941 agcagggcgt tgccccgccg cgcgacctca gggtcggcgt catggtcgag gtgccgtcgc
     3001 tgttgttcga gctgcgggag atcgcccgcg aggcggattt cctgtcgatc ggcaccaacg
     3061 acctcatgca gttcctcttc gccgccgatc gtgagaacaa gcgtgtcgcc gaccgcttcg
     3121 atccgctcgg cgtcggcgcc cttagggctc tcaggcgtat catcgaggct gctgccgaga
     3181 ccggctgccc cgtcaccgtc tgtggcgaga tgggcggcaa gccactggag accatggccc
     3241 tgatcggcct gggctatcgc ggcttctcga tgtcggctgc ctcgatcgga ccggtcaagg
     3301 cgatgttgcg cgcgctcgac gccggcaagc tgcgcgagcg catggactgg atgctggcct
     3361 ctccggaagg ggccaccaat ctgcgcccgc aactggccgc cttcgcgacc gagttcaagg
     3421 tcccggtcta gcgacgctgc ttttgcagcc gctgccgctt ttcccttttg tttcacgagt
     3481 tcatttgatg tccctccagc tccctcagga tcgtctcgac gtcatcgtgg cgcgccatgc
     3541 cgccgcgacc gacgagatca atcatgccac cgaggcggcg cgcgccgtcg agctggcgaa
     3601 ggaactggcc gagcttgatc ccgtcgtcgc ggcgatcgcc tcctggcggc aggcgcagat
     3661 ctcgctggac gaggcggagg cgctgatcgc cgatcccgcg accgattccg aattccgcga
     3721 cctcgcctat gaggagcgcg acatcgcccg cgccgagatc gaggcgcgcg cccgcgagat
     3781 cctcgtcgcc ctgctgccga aggatgccgc cgacgagcgc ggcgtcattc tcgagatcag
     3841 ggcgggcacc ggcggcgacg aggccgcgct cttcgccggt gacctcctgc gcatgtatca
     3901 gcgccacgcc gcccagaagg gctggagcct cgatgtcctc tccgagagcg agggcacggc
     3961 cggcggttac aaggaggtca tcgccgagat caacggccgc ggcgtctatg cccgtctcaa
     4021 gttcgaatcc ggcgtccacc gcgttcagcg cgtgccggat accgaggcga gcgggcgcat
     4081 ccacacctcg gctgcgacgg tggcgacgct gccgctcgcc accgatgtcg atgtcaccct
     4141 caacgaggcc gacatccgca ccgacacgct gcgctccggc ggcgccggcg gccagcacgt
     4201 caacaagacc gaatcggcgg tgcgcctgac ccatatcccg accaacaccg tcgttctggt
     4261 ccaggacgag cgctcccagc acaagaacaa ggcgcgggcc tatgaattgc tgcgcgccaa
     4321 gctcttcgac atggagcgcc tgcgcatcga tgccgagcgc tcggcggacc gacgttcgca
     4381 ggtcggttcg ggcgatcgct cggaacgcat ccgcacctat aatttcccgc aggggcgcgt
     4441 gaccgaccac cgcatcaacc tgacgctcca ccgcctgccc gaaatcatcg agggcgcgat
     4501 ggacgaactg atcgatgcgc tgatcgccga ggatcaggcg cagcggctgg cgggtctcgg
     4561 cgggtgagcg aggtaagcgc ggcgctgcgc gaggcggcgg cgcagctggc ggcggtgtcc
     4621 gatacggcgc ggctcgatgc cgaattgctg atggcgcacg cgctgggggt cgagcggccg
     4681 gcgcttctgc tcgacgcgtc gcggttcgcg cttcctgccc gattcgccga tttcgttgcg
     4741 cggcggatgc ggagcgaacc cgtcgcctat attcttggct accgcgattt ctggacggtt
     4801 cgtatcggcg tcggtccggg cgtgctgatt cccagacctg acagcgaaac gctgatcgag
     4861 gcggcgcagg ctcatttcgg cacaggcggg ccgacgcgaa ttctcgacct cgggaccggg
     4921 ccggggacgc tgctttacgc ggcgctggcc gaatggcccg cggcgaccgg tcttggcgtc
     4981 gatgccagcg atattgcgct cgactatgcc aatcggaatg cgctggcgct ggggctggag
     5041 aggcaggtcg aactgatgca gggcgactgg gcgcgcgggg ttgcgggaca attcgacctc
     5101 atcctctgca atccgcccta tatcgccgac agcgagcaat tgatgcccga tgtcgccgac
     5161 cacgagcccg cgggcgcctt gttcgcgggc gccgatggac ttgacgatta tcgccgtatc
     5221 attcctgaat tgccgcgact gatgtcgccg cgcggtgtcg cgatccttga aatcggggcg
     5281 acgcagcata tgttggtaag gcagcttgcc gaagccgcgg gctttttcgt ccggtgccgg
     5341 caggatctgg gtgggcgcga ccgggcgctt ttgctgaccc gcacctgata cgctcacaag
     5401 aattcgcttg gtttccgccg caaagcgcgg tagggggccg gcacagacct gatcagggtc
     5461 cctttggcgg tgcatggcac cgatactggt ccggcaggtt tgcttcccaa ttacgatgcg
     5521 gcacgaaacc gtggggtttc gggcaagcag tagagggcaa ggaccgcgca aggcgcgcgg
     5581 gcacgacgcg caaattggcg cggtcggcca taaggggttg gcatagctta ttagcttatt
     5641 cgacaggatg tctcagttga acatgaacaa ccggcaaagc ggtcgccgtc gcggccgcaa
     5701 caacaacagc aacaacaata accggtcgca gtcgggcggg cgcggtgggg tcgaccaggc
     5761 caaccgcatc gacagccgcg cacgcggcaa cggcgcccag atgatcgaga agtaccgcaa
     5821 ccttgcgcgc gacgcgcagc ttgccggcga tcgggtccag accgaatatt atctgcagtt
     5881 tgccgaccat tatttccgtg tcgtgagcga tttccgcgcg cggcaggaag aaaagaacgc
     5941 ccagaacggg caggatcgcg gtcacgaccg gagccgcgag attcgcggcg tcgaagattt
     6001 cgacgggcac gacgacagcg atatcgatat cgacaccgat cgcgacgata gcgacaatgg
     6061 cgacgaccgc ggcgaacgaa acgaccgcgg caacgaccgt aacgagcggg gcgaccgcag
     6121 ccagcgcgac aatcgtggca atcgcgacga cgacagccgc ggcaatcgcg aaccgcgcgg
     6181 ccagcgcgac gacgacaatc gcggcaaccg ccagccggcg cgcac
//
LOCUS       DATMSU010000090         6206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_68192, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000090 DATMSU010000000
VERSION     DATMSU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68192"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1008)
                     /locus_tag="VN034_02395"
     CDS             complement(235..1008)
                     /locus_tag="VN034_02395"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWV59475.1"
                     /translation="MSAPFRFPRFFVTSPAPCPYLAGKTERKVFTELSGNNANELNDA
                     LGRIGFRRSQSVAYRPSCTDCSACVSVRVCASEFTPSSSQKRNLRRNGDLVATACKPW
                     ATEEQYALLRSYLASRHPDGGMADMDEQDFADMVEQTPVDSYMIEYREPAADGGKGRL
                     VGCCLTDRQGDGLSMIYSFFDAHHPMREGLGTYIIADHVQRAARAALPYVYLGYWIEG
                     SARMAYKARFRPLEKLGPDGWSSVEETQSDRIAAMFELA"
     gene            complement(1243..2697)
                     /gene="pyk"
                     /locus_tag="VN034_02400"
     CDS             complement(1243..2697)
                     /gene="pyk"
                     /locus_tag="VN034_02400"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWV59476.1"
                     /translation="MVQSFTPRQRKVRILATLGPASANAEMIAELHRAGADAFRVNMS
                     HGDHAGHAKVIAAIRALEKETGRPTTILVDLQGPKLRVGTFKDGPAELVKGKPFVLDA
                     DKTPGDATRVHLPHPELFAAAEPDTRLLIDDGKLVLRVKSVAPDRIETIVEVGGKISD
                     RKGVNVPDVVVPLAALTEKDRKDLAFALEQHVDWIALSFVQRPEDVAEARRLIGGKAA
                     LLVKFEKPSGVQRIEEILELADAAMVARGDLGVELPPEAVPPLQKKIVATARRMGKPV
                     VVATQMLESMIVSPSPTRAEVSDVATAVYDGADAIMLSAETAAGAWPVEAVTMMDSIA
                     RSVESDPDYYRRLHFTETVPDATTADALAEAAGSIITTIAADAIICFTASGSTARRVA
                     RERPGAPLLVLTPKKEAARRMGLLWGAHAVPTKDIGSFEEMIAKGKRMALRHGICKAG
                     AKLVMMAGVPFGTPGSTNVLHVATLTGDELRGYS"
     gene            2807..3136
                     /locus_tag="VN034_02405"
     CDS             2807..3136
                     /locus_tag="VN034_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HWV59477.1"
                     /translation="MSCSDDQTTGDDALDSLDDAVAAAAFRRLARLLQHRSDAANIDL
                     MGLAGFCRNCLGDWIAEAGAMDKEAGRAIVHGMPTAEWKARHHTAATPEQLARMEESM
                     AKNPRPR"
     gene            3191..3433
                     /locus_tag="VN034_02410"
     CDS             3191..3433
                     /locus_tag="VN034_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HWV59478.1"
                     /translation="MSEATVSDEQLRLFIERIERLEEEKKGIADDIRDTYNEAKSQGY
                     DPKIMRQIVRLRKLPVHDRKEMEAILDVYKSALGID"
     gene            3465..3785
                     /locus_tag="VN034_02415"
     CDS             3465..3785
                     /locus_tag="VN034_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HWV59479.1"
                     /translation="MFSTTTNTVEGRPVREYLGIVTGEVIVGANLFRDLFASITDIVG
                     GRSGKYEDVLARARKEAIAEMEAEAARLGGNAVIGVDLDYEVLGQNGSMLMVSASGTA
                     VTLG"
     gene            3788..4273
                     /locus_tag="VN034_02420"
     CDS             3788..4273
                     /locus_tag="VN034_02420"
                     /inference="COORDINATES: protein motif:HMM:NF040504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family B"
                     /protein_id="HWV59480.1"
                     /translation="MHICPAAPTDAARCAEIYAPYVVDNWVSFECDPPDAVEMARRIE
                     DYGASHGWLVAEVEGQIAGYAYGSPHRTRVAYATSADVAVYVDAAFTRSGVGRQLYDA
                     LFPILKNRNVHAIFAGIALPNDASIGLHEAMGFRPVGIYREVGWKMGGWRDVGWWQRL
                     L"
     gene            4331..5077
                     /locus_tag="VN034_02425"
     CDS             4331..5077
                     /locus_tag="VN034_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWV59481.1"
                     /translation="MAGHSKFKNIMHRKGAQDKKRSSLFSKLSREITVAAKMGLPDPD
                     ANARLRAAVNAAKAQSMPKDNIQRAIDKAAGGDGENYEEIRYEGYGPGGVSLIVEALT
                     DNRNRTATNVRTAFSKNGGNLGTSGSVSHGFDRLGMISYATSAGDADTVFEAALDAGA
                     DDVESSEDGHDIWTSVDSLHEVAKALEGKLGEAEGVKLAWRPTLKSEIADEAVAQTLF
                     KLIDTLDDDDDVQTVWGNYEIPDAVMEKLG"
     gene            5244..5717
                     /gene="ruvC"
                     /locus_tag="VN034_02430"
     CDS             5244..5717
                     /gene="ruvC"
                     /locus_tag="VN034_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWV59482.1"
                     /translation="MIILGLDPSLSCTGWGVIRVEGSRISHIANGQVKTDAKAPLPDR
                     LAHLDTALAAVIADHAPMCAAVEEVFVNDNPQSTLKLAHARGVVLLGCARGGLTVTEY
                     APRLVKKAIVGTGGAAKEQVQAMLRVLLPGAKVAGADAADALAVAICHANHRPIR"
     gene            5869..>6206
                     /gene="ruvA"
                     /locus_tag="VN034_02435"
     CDS             5869..>6206
                     /gene="ruvA"
                     /locus_tag="VN034_02435"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWV59483.1"
                     /translation="MIAKLTGRLDSSNAGHAVIDVGGVGYLVEASARTLDALGPVGGE
                     ITIHTEMLVGEDFLRLLGFAKAEERDWFRLLTSVQGVGAKVALAILSALEIGDLQRAL
                     ASGDSAMIARA"
BASE COUNT         1155 a         1998 c         2002 g         1051 t
ORIGIN      
        1 gcgcgcgtgc cgccgcgcga gcggccggct gcgatcatga tgatggcgaa cgcatgcacg
       61 ggcatcccat ttcccccaat cgcttaaccc ctaagcgatg ctaaccacgg atgcgacccg
      121 gacttatcgt cctcgcgacg agctatgccc ttgaaagcgg gaaaaggtca accgctcgac
      181 cgccagaatc ggcagttgcg agccattctc aactgccctg atgaatgggt cggctcaggc
      241 gagctcgaac atcgccgcga tgcgatcgga ctgcgtttcc tcgacgctcg accagccgtc
      301 gggcccaagc ttttcgagcg ggcggaagcg cgccttgtac gccatgcgcg ccgatccctc
      361 gatccagtag ccgagataga catagggcag cgcggcgcgc gccgcgcgct ggacatggtc
      421 ggcgatgata taggtgccga gcccctcgcg catcggatgg tgcgcatcga agaagctgta
      481 gatcatcgac agcccgtcgc cctgccggtc ggtcaggcag caaccgacga ggcggccctt
      541 gccgccatcg gcggccggtt cgcgatattc gatcatatag ctgtcgaccg gggtctgctc
      601 gaccatgtcg gcgaaatcct gctcgtccat gtcggccatg ccgccgtcgg gatggcgcga
      661 ggcgagatag gagcggagca gcgcatattg ttcttcggtc gcccacggct tgcacgcggt
      721 ggcgacgagg tcgccgttgc ggcgcaggtt gcgcttctgc gagctgctcg gcgtgaattc
      781 gctcgcgcag acgcgcaccg acacgcacgc cgaacagtcg gtgcagctcg ggcgataggc
      841 gaccgactgg ctgcggcgga aaccgatgcg gccgagcgcg tcgttgagtt cgttcgcatt
      901 gttgccgctg agctcggtga acaccttgcg ctcggtcttg cccgccagat aggggcaagg
      961 cgccgggctg gtgacgaaaa agcgcggaaa acggaacggt gcggacacgc ggaaaaaacc
     1021 tccggacttg cgatgcggag ccccccgacg cgggtccgac gcgctacttg caccaactat
     1081 gccgcgcggc gtcaatggtg caaagtccat ttaccatttt taaacgtccc gttctgaatc
     1141 acgccgaaaa ttttgtgaat cagccggtaa cttttgtgcc gatttgaatc gactatgccg
     1201 aaatgatcct ccctgtgccg aagcatgggg aggattttat aatcagctat acccgcgcag
     1261 ctcgtcgccg gtgagcgtcg cgacgtggag cacgttggtc gaccccggcg tgccgaacgg
     1321 aacccccgcc atcatgacga gcttcgcgcc cgccttgcag atgccgtggc gcagcgccat
     1381 gcgcttgccc ttggcgatca tctcttcaaa gctgccgatg tccttggtcg gcaccgcatg
     1441 cgcgccccac agcaatccca tgcgccgcgc ggcttccttc ttcggcgtca gcacgagcag
     1501 cggtgcgccc ggccgctcgc gcgcgacgcg gcgcgcggtc gatcccgacg cggtgaagca
     1561 gatgatcgca tcggcggcga tcgtcgtgat gatgctgccc gccgcttcgg cgagcgcgtc
     1621 ggcggtcgtc gcgtcgggca ccgtctcggt gaaatgcagg cggcgataat aatcggggtc
     1681 gctttcgacc gagcgcgcga tcgaatccat catcgtgacc gcctcgaccg gccaggcgcc
     1741 cgccgcggtc tcggccgaca gcatgatcgc gtcggcgccg tcatagaccg cggtcgccac
     1801 gtcggacacc tccgcacgcg tcggcgacgg cgagacgatc atcgattcga gcatctgcgt
     1861 cgcgacgacc acgggcttgc ccatgcggcg cgcggtcgcg acgatctttt tctggagcgg
     1921 cggcaccgct tcggggggca gctcgacccc cagatcgccg cgcgcgacca tcgccgcgtc
     1981 ggcgagctcg agaatttcct cgatccgctg cacgcccgac ggcttttcga acttgacgag
     2041 caacgccgcc ttgccgccga tcaggcgccg cgcctcggcg acatcctcgg gccgctggac
     2101 gaacgacagc gcgatccagt cgacatgctg ttcgagcgcg aaggccaggt ccttgcggtc
     2161 cttttcggtc agcgccgcga ggggaacgac gacgtcgggg acgttgacgc ccttgcggtc
     2221 cgaaatcttg ccgccgacct ccacgatggt ttcgatccgg tccggcgcaa cgctttttac
     2281 ccgcaacacc agtttgccgt cgtcgatcag caggcgcgta tcgggttcgg ccgccgcgaa
     2341 caattcggga tgcggcaagt ggacgcgcgt cgcgtcgccc ggcgtcttgt cggcgtcgag
     2401 cacgaagggc ttgcccttca ccagctcggc ggggccgtcc ttgaacgtcc cgacgcgcag
     2461 cttcggcccc tgcaggtcga cgaggatcgt cgtcgggcgg ccggtttcct tttcgagcgc
     2521 gcggatcgcg gcgatcactt tcgcatgacc cgcatggtcg ccgtggctca tattgacgcg
     2581 aaaggcgtcg gcgcccgcgc ggtgcagttc ggcaatcatc tcggcattag cgctggcggg
     2641 accgagggtc gccaggatgc gaaccttgcg ctggcggggg gtaaagctct gaaccacaaa
     2701 tgctccgatg acttgggtag gtgggggtct gggacgctag ggcaatgccg ccttgatgct
     2761 gccaacgcaa caaccgtata gctattcatc atacaaagga gaaaccatgt cctgcagcga
     2821 cgatcagaca accggcgacg atgccctcga cagcctcgac gatgcggtcg cagcggccgc
     2881 attccgccgc ctcgctcgct tgctccagca tcgcagcgac gccgcgaata tcgatctgat
     2941 ggggctcgcc ggcttttgcc gcaactgcct cggcgactgg attgccgagg cgggcgccat
     3001 ggacaaggaa gccggccgcg cgatcgtcca cggcatgccg accgccgagt ggaaagcgcg
     3061 ccatcatacc gcagccaccc ccgaacagct cgcgcggatg gaagaaagca tggcgaagaa
     3121 tccccgacct cgctagggac gcgccgaggg cgattctcgc ctccaatcat ccaatccagc
     3181 ggagtataaa atgagcgaag ccaccgtgtc cgacgaacaa ttgcgccttt tcatcgagcg
     3241 catcgagcgt ctggaggaag agaagaaggg catcgccgac gacattcgcg acacctataa
     3301 tgaagcgaaa tcgcagggtt acgacccgaa gatcatgcgc cagatcgttc gcttgcgtaa
     3361 attgccggtt cacgaccgca aggaaatgga agcgatcctc gacgtttata agtcggcgct
     3421 cgggatcgac tgaagtctat ccaccaatcc acaggagagt gacgatgttc agcaccacga
     3481 ccaacactgt cgaaggccgt ccggtccgcg aatatctagg catcgtcacc ggcgaggtga
     3541 tcgtcggcgc caacctgttc cgtgacctgt tcgcgtcgat caccgacatc gtcggcggcc
     3601 gctcgggcaa atatgaggat gtcctcgccc gcgcgcgcaa ggaagcgatc gccgagatgg
     3661 aggccgaggc ggcacgcctc ggcggcaatg cggtgatcgg cgtcgacctc gactatgagg
     3721 tgctcggcca gaatggctcg atgctgatgg tcagcgcgtc gggaaccgcg gtgacactgg
     3781 ggtgagggtg cacatctgtc cagccgcgcc gaccgacgcc gcgcgctgtg ccgagattta
     3841 tgcaccctat gtcgtcgaca attgggtcag tttcgaatgc gatccgcccg acgcggtgga
     3901 aatggcgcgc cgtatagaag attatggggc cagccatggc tggctggtcg cggaggtgga
     3961 aggccaaatt gcgggatatg cctatggctc gcctcaccgc acgcgagtgg cctacgccac
     4021 ctcggctgat gttgcggttt atgttgatgc ggcttttaca cgttcgggag ttggacggca
     4081 gctttatgac gcgctgtttc cgattctgaa gaatcgcaac gtccatgcaa tctttgccgg
     4141 aattgccttg ccgaatgacg ccagcatcgg cttgcacgag gcaatgggct tcaggccggt
     4201 cggaatctac cgcgaggtcg gctggaaaat gggcggctgg cgcgatgtcg ggtggtggca
     4261 gcggttgctt tgacgttcgg tcctgatgta agcgcgcctc cagttcctcc cagccaaaaa
     4321 gaagagagcc atggccggcc atagtaaatt caagaacatc atgcaccgca agggtgcgca
     4381 ggacaaaaag cgcagcagcc tcttttcgaa gctcagccgc gaaatcaccg tcgcggcgaa
     4441 gatgggtctg cccgaccccg atgcgaatgc ccgccttcgg gcggcggtca acgccgccaa
     4501 ggcgcagtcg atgcccaagg acaatatcca gcgcgcgatc gacaaggccg cgggcggtga
     4561 cggcgagaat tacgaagaaa tccgctacga aggctatggc ccgggcggcg tgtcgctgat
     4621 cgtcgaggcg ctgaccgaca accgcaaccg caccgcgacc aacgtccgca ccgccttttc
     4681 gaaaaatggc ggcaacctcg gcacgtcggg tagcgtcagc cacggcttcg accggctcgg
     4741 catgatcagc tatgccacca gcgccggcga cgccgacacg gtgttcgaag ccgcgctcga
     4801 cgccggcgcc gacgacgtcg aatcgtcgga ggacggccac gacatctgga ccagcgtcga
     4861 cagcctgcac gaagtcgcca aggcgctcga aggcaagctc ggcgaggccg aaggcgtcaa
     4921 gctcgcctgg cgcccgacgc tgaagagcga gatcgccgac gaggcggtgg cgcagacgct
     4981 gttcaagctg atcgacacgc tcgacgacga cgacgacgtc cagaccgtgt ggggcaatta
     5041 cgaaatcccc gatgcggtga tggagaaact gggttgatga aatcctctcc ccttggggga
     5101 gaggatagga aggcttgcca gcttgtctgg ctagccggac tcggtgaggg aatgtggcgc
     5161 ggcccacccc tctcccagct gcgactagcg ggcaaagccc gcgagtctgc gcatccctct
     5221 cccccagggg gagaggaaat gtcatgatca tcctcggcct cgacccctcg ctcagttgca
     5281 ccggctgggg cgtcatccgc gtcgagggca gccggatcag ccatatcgcc aacgggcagg
     5341 tgaagaccga cgccaaggcg cccctgcccg accggctcgc gcacctcgac accgcgctcg
     5401 cagcggtgat cgccgatcat gcgccgatgt gcgccgcggt cgaggaagtc ttcgtcaacg
     5461 acaatccgca atcgacgctg aagctcgccc atgcacgcgg cgtcgtcctg ctcggctgcg
     5521 cgcgcggcgg gctgaccgtc accgaatatg ccccgcgcct cgtcaaaaag gcgatcgtcg
     5581 gaaccggcgg cgcggcaaag gagcaagtgc aggcaatgct gcgcgtcctg ctccccggcg
     5641 cgaaggtcgc gggcgccgac gcggcggatg cgttggccgt cgcgatctgc catgcgaacc
     5701 atcggccgat ccgctaattt tcctttccgt tcgccccgag cttgtcgaag ggctgtcctt
     5761 ctcttaagga gaacaaagag tgaaagaacg gcgcccttcg actgcctgcc tgcggcagcc
     5821 gctcaggaca ggcttcaagc tcagcgcgaa cggctatttg agggctcaat gatcgccaaa
     5881 ctcaccggaa ggctcgacag cagcaacgca ggccatgcgg tgatcgacgt cggcggcgtc
     5941 ggctatctcg tcgaggcatc ggcgcggacg ctcgacgcgc tcggcccggt cggcggtgag
     6001 atcaccatcc acaccgaaat gctcgtcggg gaggatttcc tgcgcctgct cggcttcgcc
     6061 aaggccgagg agcgcgactg gttccggctg ctgaccagcg tccagggcgt cggcgccaag
     6121 gtcgcgcttg cgatcctctc ggcgctcgaa atcggcgacc tgcaacgcgc gctcgcgagc
     6181 ggcgacagcg cgatgatcgc gcgcgc
//
LOCUS       DATMSU010000091         4953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_30272, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000091 DATMSU010000000
VERSION     DATMSU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30272"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VN034_02440"
     CDS             complement(<1..1135)
                     /locus_tag="VN034_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HWV59484.1"
                     /translation="MAGLGDAPFDAGGRHAARGAPDQNDDAATVADSHGRSLILASVA
                     RDARLDKKFLLLITLSAAIATLGLLQNSAAVVIGAMLVSPLLGPIMGIGFGLATIESN
                     LIKRSLVTMAAGMAVAVLVAMLLVWLSPIADVTSELRARTQPTLLDLGVAVVGGIAGV
                     YAIMRKLSGVMVGVAIATALVPPLSTIGFGLATGRADFALGAALLFLTNTLAIAFAAT
                     IVARLNRFGPSLTRQHTAMQVVGIIVTLGILSIPLALSLNDIARELRARTAVQAELGR
                     LLGKDDRIDSLNVRLEDDDVAVDGVVLVDQYASRLNTTLADKVAGQLDRDVRVSIVQL
                     RQQTNAAAEYEEQLGRRLAALEQREDDSRAILAGLTVGELLPRD"
     gene            complement(1169..2098)
                     /locus_tag="VN034_02445"
     CDS             complement(1169..2098)
                     /locus_tag="VN034_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWV59485.1"
                     /translation="MKTLVWVEHDGSAVKDATLAAVTAASKLGEVHLLVAGEGVDGVA
                     KAAAAIAGVGKVHVADNAAFGHNLPENVAPLVAELMASHDAFVAPATTTGKNIAPRVA
                     ALLDVMQISEILSVEGPKTFTRPIYAGNAIATVESSDAKLVLTVRGTAFEKAATSGGS
                     GAVEAVSAGGDTGISSFVGAEIAKQDRPELTSAKIIVSGGRALGSSEKYEEVIVPLAD
                     KLGAALGASRAAVDAGYVPNDYQVGQTGKIVAPEVYFAIGISGAIQHLAGMKDSKTIV
                     AINKDEDAPIFQVADFGLVADLFNAVPELTGKI"
     gene            complement(2095..2844)
                     /locus_tag="VN034_02450"
     CDS             complement(2095..2844)
                     /locus_tag="VN034_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWV59486.1"
                     /translation="MKILVPVKRVLDYNVKPRVKADGTGVDLANVKMSMNPFDEISVE
                     EAIRLKEKGVATEIIAVSVGPAKAQETLRTALAMGADRAILVQTDDAVEPLALAKIFK
                     AIADAEQPGLVILGKQAIDDDNNQTGQMLAALTGWAQGTFASAVNVDGDSVNVTREVD
                     GGLETVKLKLPAIVTTDLRLNEPRYASLPNIMKAKSKPLDTKSPADYGVDTAPRVKTV
                     KVSEPPVRSAGVKVADVDELVAKLKALGVHS"
     gene            complement(3089..4294)
                     /gene="sucC"
                     /locus_tag="VN034_02455"
     CDS             complement(3089..4294)
                     /gene="sucC"
                     /locus_tag="VN034_02455"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWV59487.1"
                     /translation="MNIHEYQAKELLAKFGVAVPKGIAAMTVEEAVAAAKQLPGPLYV
                     VKSQIHAGGRGKGKFKELGPDAKGGVRLAKTIEEVEAHAKDMLGNTLVTIQTGEAGKQ
                     VNRLYITDGADIKQEFYLALLVDRATSRIAVVASTEGGMDIETVAHDTPEKIHTITID
                     PATGIQPHHGRSVAAALGLSGDLAKQAAKVLGGLYAAFLGTDAEQIEINPLAVCEGAN
                     GDELLVLDAKVAFDGNAMFRHKDLAELRDLTEEDPAEVEASEYDLAYIKLDGNIGCMV
                     NGAGLAMSTMDIIKLNGEFPANFLDVGGGASKEKVTAAFKIILKDPAVKGILVNIFGG
                     IMKCDIIADGIVAAAKEVNLSVPLVVRLEGTNVQQGKDILANSGLPIVAANDLGDAAK
                     KIVAEVRAA"
     gene            4501..>4953
                     /locus_tag="VN034_02460"
     CDS             4501..>4953
                     /locus_tag="VN034_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59488.1"
                     /translation="MRIKLLVLTWLTLAGPVAATAQVNQSIDVQIVEAPKIVRIGDAD
                     QLVYELHLTNFSSHPLELNALAIKAGDTPIKTYSRDELPTAIDLVGPPEANSRIIPIG
                     RRAVVYIGAPVPAAYLRGSISHSLTLSKIDTNEGDFTISGGTATPEAAA"
BASE COUNT          838 a         1595 c         1612 g          908 t
ORIGIN      
        1 ggtcgcgcgg cagcaattcg ccgacggtca gcccggcaag gatcgcgcgg ctatcgtcct
       61 cgcgctgttc gagcgccgcg aggcggcgcc ccagctgttc ctcatattcg gcggcggcat
      121 tggtctgctg ccgcagctgg acgatgctga cgcgcacgtc gcggtcgagc tggcccgcga
      181 ccttgtcggc gagcgtcgtg ttgagccgcg aggcatattg gtcgaccaaa acgacgccgt
      241 cgaccgcgac gtcatcatcc tcgagccgga cgttcaggct gtcgatccgg tcgtccttgc
      301 cgagcaaccg gccaagctcg gcctgcaccg ccgtccgcgc gcgcagttcg cgcgcgatat
      361 cgttgagcga aagcgcgagc gggatcgaaa gaatgccgag cgtgacgatg atgccgacca
      421 cctgcatcgc cgtgtgctgc cgcgtcagcg aaggaccgaa gcgattgagc cgcgcgacga
      481 tcgtcgcggc gaaggcgatc gcgagcgtgt tggtgaggaa gagcagcgcg gcgccgagcg
      541 cgaagtccgc ccgcccagtc gcgagaccga agccgatcgt cgaaagcggc ggcaccagcg
      601 cggtcgcgat ggcgacgccg accatcaccc ccgacagctt gcgcatgatc gcatagacgc
      661 ccgcgatgcc gccgacgacc gcgacgccaa ggtcgagcag cgtcggctgt gtccgggcgc
      721 gaagttcgga tgtgacgtcg gcgatcggcg agagccagac gagcagcatc gcgacgagga
      781 ccgcgaccgc catccccgcc gccatcgtca ccagcgagcg cttgatcaga ttgctttcga
      841 tcgtcgcgag gccgaagccg atccccatga tcgggccgag cagcggcgac acgagcatcg
      901 cgccgatcac caccgccgcc gaattctgca agaggccgag cgtcgcgatc gccgccgaca
      961 gcgtgatgag cagcaggaat ttcttgtcga gccgcgcatc gcgcgcgacg ctcgccagaa
     1021 tcaacgagcg accgtggctg tccgccacgg tcgctgcgtc gtcattctgg tccggggcgc
     1081 cgcgcgccgc gtggcgaccg ccggcatcga acggcgcatc gcccagtccg gccaccccgg
     1141 gatgatttcc ggccataggg gcggacggtt agatcttgcc cgtcagttcg ggcacggcat
     1201 tgaagagatc ggcgacgagg ccgaagtcgg cgacctgaaa gatcggggcg tcctcgtcct
     1261 tgttgatcgc gacgatcgtc ttcgaatcct tcatgcccgc gagatgctgg atcgcacccg
     1321 agatgccgat cgcgaaatag acctccggcg cgacgatctt gccagtctgg ccgacctgat
     1381 aatcgttggg gacatagccc gcatcgaccg cggcgcgcga cgcgccgagc gcggcgccga
     1441 gcttgtcggc gagcgggacg atgacctctt catatttctc gctcgaaccg agcgcgcggc
     1501 cgcccgacac gatgatcttc gccgaggtga gttcgggacg atcctgcttg gcgatttcgg
     1561 cgccgacaaa gctcgagatg ccggtgtcgc cgccagccga gacggcctcg accgcgcccg
     1621 aaccgcccga cgtcgcagcc ttttcaaagg cggtgccgcg aacggtgagg acgagcttgg
     1681 catccgacga ttcgacggtc gcgatcgcgt tgccggcata gatcgggcgg gtgaaggtct
     1741 tcggaccctc gaccgagagg atctcgctga tctgcatcac gtcgagcagc gcggcgacgc
     1801 gcggcgcgat atttttgccg gtcgtggtgg cgggcgcgac gaacgcgtcg tggctcgcca
     1861 tcagttcggc gacgagcggc gcgacatttt cgggcaaatt gtgaccgaaa gcggcgttgt
     1921 cggcgacatg gaccttgccg acaccggcga tcgcagccgc ggccttggcg acgccatcga
     1981 cgccctcgcc cgcgacgagc aggtggactt cgccgagctt cgacgcggcg gtcaccgcgg
     2041 cgagcgtggc atccttgacg gcgctgccgt catgttcgac ccaaaccaga gttttcatga
     2101 atgcactccc agcgctttga gcttggcaac cagttcatcg acatcggcga ccttgacgcc
     2161 ggccgagcgg acgggcggtt cggacacctt gaccgtcttg acgcgcggtg cggtgtcgac
     2221 gccgtaatcg gcgggcgact tggtatcgag cggcttcgac ttcgccttca tgatattcgg
     2281 cagcgacgca tagcgcggct cgttgaggcg aaggtcggtg gtgacgattg cgggaagctt
     2341 gagcttcacc gtttcgaggc cgccgtcgac ttcgcgcgtc acattgacgc tgtcgccatc
     2401 gacattgacc gcgctggcga aggtgccctg cgcccagccg gtcagcgcag cgagcatctg
     2461 gccggtctgg ttgttgtcgt cgtcgatcgc ctgcttgccg aggatcacca gacccggctg
     2521 ttcggcgtcg gcgatcgcct tgaagatttt cgcgagcgcc agcggctcga cggcatcgtc
     2581 ggtctggacg agaatggcgc ggtcggcgcc catcgcgagc gcggtgcgca gcgtttcctg
     2641 tgccttcgcc gggccgacgg aaacggcgat gatctcggtc gcgacgccct tttccttcag
     2701 gcgaatcgct tcttcgaccg agatctcgtc gaacgggttc atcgacatct tcacattggc
     2761 aagatcgacg ccggtgccgt cggccttcac ccgcggcttc acgttgtaat cgagcacccg
     2821 cttcacgggg acgaggattt tcatgggctc aaagctcctc tcagcaaatt gtgcactgcg
     2881 ccatagttgc gctttacgta aacgtcaacc agcagtcccc ataacccctc tccccctggg
     2941 gagaggatag ggagacttgg tccgcaagga cccagtcgga cttggtgagg gcttgcgcct
     3001 tgggcgtcca taccctcacc cagcttcgac cagccagcaa gctggcaagt cttcgcatcc
     3061 ctctccccca aggaaagagg gaaatagatc aggccgcgcg aacctcggcg acgattttct
     3121 ttgccgcgtc gcccagatcg tttgccgcca cgatcggcag gcccgaattg gcgaggatgt
     3181 ccttgccttg ctggacgttg gtgccttcga ggcggacgac gagcggaacc gagaggttca
     3241 cttccttcgc cgcggcgacg atgccgtcgg cgatgatgtc gcacttcatg atgccgccga
     3301 aaatattgac gagaatgccc ttcacggcgg ggtccttgag gatgatcttg aacgccgcgg
     3361 tgaccttttc cttgctcgcg ccgccgccga cgtcgaggaa gttcgccggg aattcgccgt
     3421 tgagcttgat aatgtccatc gtcgacatcg cgagacccgc gccattgacc atgcagccga
     3481 tgttgccgtc gagcttgatg taggcgaggt catattccga cgcttcgacc tcggccggat
     3541 cttcctcggt caggtcgcgc agctcggcga gatccttgtg gcggaacatc gcattgccgt
     3601 cgaacgccac cttggcgtcg aggacgagca attcgtcgcc gttcgcgcct tcgcagacag
     3661 ccagcgggtt gatttcgatc tgctcggcat cggtgccgag gaaggcggca tagaggccgc
     3721 caagcacctt ggcggcctgc ttggcgagat cgcccgacag gccgagcgcc gcggcgacgc
     3781 tgcggccatg gtgcggctgg attcccgtgg cgggatcgat cgtgatcgtg tggatctttt
     3841 cgggcgtgtc gtgcgcgacg gtttcgatgt ccatgccgcc ctcggtcgag gcgacaaccg
     3901 cgatgcggct ggtggcgcgg tcgacgagca aagcgaggta gaactcctgc ttaatgtcgg
     3961 cgccgtcggt gatgtacagg cggttgacct gcttgccggc ttcgccggtc tggatcgtca
     4021 ccagcgtgtt gccgagcatg tccttggcgt gcgcttcgac ttcctcgatc gtcttggcga
     4081 ggcggacgcc gcccttcgcg tccgggccga gttccttgaa cttgcccttg ccgcggccac
     4141 ccgcgtggat ctgcgatttc acgacataga gcggcccggg gagctgcttc gccgcggcga
     4201 cggcttcctc gacggtcatc gcggcaatgc ccttggggac ggcaacgcca aatttcgcga
     4261 gcagttcttt tgcctgatat tcgtggatgt tcatgaggtc tgccgggcct ttcgactctg
     4321 tgaagggaga ggatcggcgc cgcctaagca caagtttggc gccaattcta cccccgggta
     4381 gccccagata ttcttgtgcc cggcaaaggc ttgcggcgag ccggtccgtc tttcggatga
     4441 cgccccgggc ccgagttgat aatcgggcac ggtcgaggga tcgggatgag ggacgcgaag
     4501 atgcggataa agcttcttgt tttgacatgg ttgacgctcg ccggtccggt cgccgcgacg
     4561 gcacaagtga accagtcgat cgatgtacag atcgtcgagg cgccgaaaat cgttcggatc
     4621 ggcgacgccg accagctcgt ctatgaactc cacctcacca atttttccag ccatccgctc
     4681 gagctgaacg cgctcgcgat caaggcaggc gacacgccga tcaagaccta tagccgggac
     4741 gaactgccca cggcgatcga tctcgtcggg ccgcccgaag ccaattcgcg gatcattccc
     4801 atcggccgcc gcgctgtcgt ctatatcggc gcgcccgtcc ccgccgccta tctccgcgga
     4861 tcgatctcgc acagcctgac gctgagcaag atcgacacca atgaaggtga tttcacaatt
     4921 tcgggcggca cggcaacgcc ggaggccgcc gcc
//
LOCUS       DATMSU010000092         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_39824, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000092 DATMSU010000000
VERSION     DATMSU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39824"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VN034_02465"
     CDS             complement(<1..500)
                     /locus_tag="VN034_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HWV59489.1"
                     /translation="MSSFIPYAPLNVPKPFGEEIWIVDGPEIRMDYGPASMPFPTRMT
                     VVRLPGGRLWIHSPIAPDDALFAAIDRLGEVAWLVAPNSIHYWYVADWQARYPAAHTF
                     AVPGLAEKAKRDFRIDALLEGPAMPLPDGIAGVLVPGTMVSEAVFFHPASRTVILTDL
                     IENFEPA"
     gene            complement(566..1024)
                     /locus_tag="VN034_02470"
     CDS             complement(566..1024)
                     /locus_tag="VN034_02470"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV59490.1"
                     /translation="MMATTITGGCRCGQLRYSFEQEGPLLNYCCHCLDCQKSTASAFA
                     DQIIVATDALVLTGRAVTFSTERPSGGTTTNQHCPECFSRVCNANDVYPAMTIVRAGT
                     LDDPSELEPFAHIWVKQKRGWIVIADGVPTFEESPDPMEFMRFVMERRGS"
     gene            complement(1021..1638)
                     /locus_tag="VN034_02475"
     CDS             complement(1021..1638)
                     /locus_tag="VN034_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59491.1"
                     /translation="MRKFITLAMIGLLAACKPAAEKAPAPDASDADPAAPPVPAAKND
                     GPNAATTAISLDGEGLRFVDKTSGKASLLAFGVPRAQAETALANVAGKEDDRSTNAEC
                     GAGVMEFTRYDAMTLNFQDGKFAGWFLGNEPGADSYSTMSGIAVGTTRAKAAESVTIV
                     DIEDSTLGEEFSIGTGDKVIGGMFAAPGDTAKIDALFAGVNCFFR"
     gene            complement(1640..2263)
                     /locus_tag="VN034_02480"
     CDS             complement(1640..2263)
                     /locus_tag="VN034_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HWV59492.1"
                     /translation="MNALAAIPPAQRRLLGLLLALLLLAQIDQPYPEVALLQHIPTAL
                     LLALSPWLLRRWPMSTASVACIVVFMALHTLGGRYAYSNVPYDEWARALTGTSLSDAF
                     GWTRNHYDRLVHFAFGALSVIPVAEIARRWGGLARRGAALSVLCWVLAISCLYEIFEW
                     LLTIVAAGETADRYNGQQGDIWDAQKDMALALLGAILVLAIPRRDRR"
     gene            complement(2475..3440)
                     /locus_tag="VN034_02485"
     CDS             complement(2475..3440)
                     /locus_tag="VN034_02485"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWV59493.1"
                     /translation="MLAEREFPYDELAAVASSRSQGDEIEIGDTGKTVKCQNIENFDW
                     SGWDMAIFAIGSDATAIHAPKAAAAGCVVIDNSSLYRMDPDVPLIVPEVNPEAIDGYT
                     AKNIIANPNCSTAQMVVALKPLHDAATIKRVVVSTYQSVSGAGKAGMDELWNQTRQIF
                     VGDEKDVQKFTKQIAFNVIPHIDKFLDDGSTKEEWKMVVETKKILDPKIKVTATCVRV
                     PVFVGHSEAINIEMEDELSAEDAQRILREAPGVVLHDKREDGGYITPVECVGDFATFV
                     SRVREDPTVDNGLNLWCVSDNLRKGAALNAVQIAELLGRRHLKKG"
BASE COUNT          565 a         1098 c         1166 g          615 t
ORIGIN      
        1 gcgggttcga aattctcgat caggtcggtc agaatgacgg tgcgcgaggc tgggtggaaa
       61 aacaccgctt cgctgaccat cgtgccgggc acgagcacgc cggcgatccc gtcgggaagc
      121 ggcatcgccg ggccttcgag cagcgcgtcg atgcggaaat cgcgcttcgc cttttccgcg
      181 agccccggca ccgcgaaggt gtgcgccgcg ggatagcgcg cctgccagtc ggcgacatac
      241 caataatgga tgctgttggg cgcgacgagc cacgcgacct cacccagccg gtcgatcgcg
      301 gcgaacagcg catcgtcggg cgcgatcggc gagtggatcc acagccgccc gccgggcagc
      361 cgcaccaccg tcatccgcgt cggaaagggc atcgacgcgg ggccatagtc catgcggatt
      421 tcggggccgt cgacgatcca gatctcctcg ccgaacggct tgggcacgtt gagcggcgca
      481 taggggatga agctggacat ggacgacagg gtatagatgc ggcggcgcgg tttcaattcg
      541 gcggcgggta tcgagaacgg atgcctcagg agccccggcg ctccatgacg aagcgcatga
      601 actccatcgg atcggggctt tcctcaaagg tgggcacgcc gtccgcaatg acgatccatc
      661 cgcgcttttg cttcacccag atatgcgcga agggttcgag ctccgacggg tcgtcgagcg
      721 ttccggcgcg cacgatcgtc atcgccggat agacgtcatt cgcattgcac acgcggctaa
      781 aacattcggg gcaatgctgg ttggtcgtgg tgcctcccga ggggcgctcg gtcgagaagg
      841 tgacggcccg ccccgtcagc accagcgcgt cggtcgcgac gatgatctgg tcggcaaagg
      901 cgctcgcggt cgatttctgg caatcgaggc agtggcagca atagttgagc agcggccctt
      961 cctgctcgaa gctgtaacgc agttgcccgc agcggcagcc gccggtgatc gtcgtcgcca
     1021 tcatcggaag aagcagttca cgccggcgaa cagcgcgtcg atcttcgccg tatcccccgg
     1081 cgcggcgaac atgccgccga tgaccttgtc acccgtgccg atgctgaact cttcgccgag
     1141 cgtgctgtcc tcgatgtcga cgatcgtcac cgattcggcc gccttggcgc gcgtggtgcc
     1201 caccgcgatg ccgctcatcg tcgaataact gtcggcaccg ggttcgttgc cgaggaacca
     1261 gccggcgaat ttgccgtcct ggaaattgag tgtcatcgcg tcgtagcggg tgaattccat
     1321 cacgcccgcg ccgcattccg cattggtgct gcggtcgtcc tccttgcccg cgacgttcgc
     1381 caaggccgtt tcggcctgcg cgcgcgggac gccgaaggcg agcaggctcg ccttgccgct
     1441 ggtcttgtcg acgaagcgca gcccttcgcc atcgaggctg attgcggtgg tcgcggcgtt
     1501 cgggccgtcg ttcttcgctg caggtaccgg cggcgcggcg ggatcggcgt cggacgcatc
     1561 tggcgcgggc gctttctcgg ccgccggctt gcacgccgcg agcaggccga tcatcgcgag
     1621 ggttatgaac ttgcgcatat tatctcctgt cgcggcgcgg gattgcgagc accagtatcg
     1681 cacccaatag cgcgagcgcc atatcctttt gcgcgtccca gatatcgcct tgctgcccgt
     1741 tatagcggtc ggcggtctcg cccgccgcga cgatggtgag cagccattcg aaaatctcgt
     1801 aaagacacga tatggcgaga acccagcaga gcacgctcag ggcggcgccg cgccgcgcga
     1861 gtccgcccca gcgtcttgcg atttcggcga cggggatcac cgacagcgcg ccgaaggcga
     1921 aatggacgag gcggtcgtaa tggttgcgcg tccagccgaa ggcgtcggag aggctcgtgc
     1981 ccgtcagcgc gcgggcccat tcgtcatagg ggacattgct gtaggcgtag cggccgccga
     2041 gcgtgtggag cgccatgaag acgacgatgc acgcgaccga cgcggtcgac atcggccagc
     2101 ggcgaagcag ccacggggat agcgcgagca gcagcgccgt cgggatatgc tggagcagcg
     2161 cgacctcggg atagggctga tcgatctgcg cgaggagcag cagcgcgagg agcaggccga
     2221 gcaggcggcg ttgcgcgggc gggatggcgg cgagggcatt cacccccgct ccttatccgt
     2281 tcgcatcgag cgaagtcgag atgccacacg cgcgcgccga cgccaagccg atcctccctg
     2341 tgccgcaggc atggggaggt ggcagcgcga agcgctgacg ggaggggctt tttggcgcga
     2401 ggtcgcgccc ctccaccacc gcctgcggcg gcggtccccc tccccatcgc ttcgcgacag
     2461 ggaggaagca gaagtcagcc cttcttcagg tgccggcggc cgagcagctc agcgatctgc
     2521 accgcgttga gcgctgcgcc cttgcggagg ttgtcgctga cgcaccagag gttgaggccg
     2581 ttatcgaccg tcggatcctc acgcacgcgg ctgacgaaag tcgcgaagtc gccgacgcat
     2641 tcgacggggg tgatgtagcc gccgtcctcg cgcttgtcgt ggagcacgac gcccggcgct
     2701 tcgcgcagga tgcgctgcgc gtcctcggcc gacagttcat cctccatctc gatgttgatc
     2761 gcctcggagt ggccgacgaa gacggggacg cggacgcagg ttgcggtgac cttgatcttg
     2821 ggatcgagga tcttcttggt ctcgacgacc atcttccatt cctccttggt cgagccgtcg
     2881 tcgaggaatt tgtcgatgtg cgggatgacg ttgaaggcga tctgtttggt gaacttctgc
     2941 acatccttct cgtcgccgac gaagatctgg cgcgtctggt tccacagctc gtccatgccc
     3001 gccttgcccg cgcccgacac cgactgatag gtcgagacga cgacgcgctt gatcgtcgcg
     3061 gcgtcgtgca gcggcttcag cgcgacgacc atctgcgcgg tcgagcaatt ggggttcgcg
     3121 atgatgttct tcgccgtata gccgtcgatc gcttcgggat tcacctcggg cacgatcagc
     3181 ggcacgtcgg ggtccatgcg gtagagcgag ctgttatcga tgacgacgca gcccgccgcg
     3241 gcggccttcg gcgcgtggat cgcggtcgcg tcgctgccga tcgcgaagat cgccatgtcc
     3301 caccccgacc agtcgaaatt ctcgatattc tggcacttca cggttttacc ggtatcgccg
     3361 atttcgatct cgtcgccctg gctgcgcgac gatgcgaccg ccgccagttc gtcgtaggga
     3421 aattcgcgct cggcgagaat ggcg
//
LOCUS       DATMSU010000093         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_77454, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000093 DATMSU010000000
VERSION     DATMSU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77454"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VN034_02490"
     CDS             <1..318
                     /locus_tag="VN034_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWV59494.1"
                     /translation="GWAAGIERLAMLLADDVIDTRLDAVIAVEDDGQLAFAMRALAAL
                     RNGGFATEIVASGSPRKRFDKAAKIPAHALISIGSRDGALHLNVRGDGDRAKAVEGLV
                     RAL"
     gene            318..1391
                     /gene="prfA"
                     /locus_tag="VN034_02495"
     CDS             318..1391
                     /gene="prfA"
                     /locus_tag="VN034_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWV59495.1"
                     /translation="MTSVSSKQIDAIIERHAALQARMATGDMAPADFVAASKEFAELE
                     PVAKAAAEVTRLRGELISLNEMLADPEMKAMAAEEIGAIEDALPAAEHDLAIKLLPKD
                     VADERAAMLEIRAGTGGDEAALFAGDLLRMYSRFADTQGWKVEIISANEAEVGGYKEV
                     VASVSGQGVFAKLKFESGVHRVQRVPATESQGRIHTSAATVAVLPEAEEVDVAINDSD
                     LKIDIYRASGAGGQHVNTTDSAIRITHIPSGLVVIQQDQRSQHKNRAKAMQVLRARLY
                     DLEREKIHSAEASARKSMVGSGDRSERIRTYNFPQGRVTDHRINLTLYKLDEMMQGLV
                     LDEIIDALTAEHQAKLLAAEGMA"
     gene            1466..1714
                     /locus_tag="VN034_02500"
     CDS             1466..1714
                     /locus_tag="VN034_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59496.1"
                     /translation="MIKMFSTIAVTGLMALAMSGVSQAQERTFRKMTSGQPAALQGAM
                     TQSQARKACRTEMRGTRESKASLNKKMITCVNLKMQGN"
BASE COUNT          341 a          594 c          653 g          261 t
ORIGIN      
        1 ggctgggcgg cggggatcga gcggctggcg atgctgctcg ccgacgatgt catcgacacc
       61 cggctcgatg cggtgattgc ggtcgaggac gacggccagc tcgcgttcgc gatgagggcg
      121 ctcgccgcgc tgcgcaatgg cggcttcgcg accgaaatcg tcgcgagcgg ttcgccgcgc
      181 aagcgcttcg acaaggcggc gaagattccg gcgcacgcgc tgatttctat cggctcgcgc
      241 gacggcgcgc ttcacctcaa cgtccgcggt gacggcgatc gggcgaaggc ggtcgagggg
      301 ctcgtccggg cactctgatg acctccgttt cctccaagca gatcgacgcg atcatcgaac
      361 gccacgccgc gctgcaggcg cgcatggcga cgggcgacat ggcgcccgcc gacttcgtcg
      421 cggcgtcgaa ggagttcgcc gagctcgaac ccgtcgcgaa agccgccgcc gaagtcacac
      481 ggctgcgcgg cgagctcatc tcgctgaacg agatgctcgc cgatcccgag atgaaggcga
      541 tggcggccga ggagattggc gcgatcgagg acgcgctgcc cgcggccgag catgatcttg
      601 cgatcaagct tcttcccaag gatgtcgccg acgaacgcgc ggcgatgctc gaaatccgcg
      661 ccggcaccgg cggcgacgag gcggcgctgt tcgcgggcga tctgctgcgc atgtacagcc
      721 gcttcgccga tacgcagggg tggaaggtcg agatcatctc ggcgaacgag gccgaggtcg
      781 gcggctataa ggaagtcgtc gcgtcggtga gcgggcaggg cgtcttcgcg aagctcaagt
      841 tcgaaagcgg cgtccaccgc gtccagcgcg tccccgcgac cgaaagccag ggccgcatcc
      901 acaccagcgc cgcgaccgtc gcggtgctgc ccgaggccga ggaggtcgac gtcgcgatca
      961 acgacagcga cctcaagatc gatatctacc gcgcgagcgg cgcgggcggg cagcatgtca
     1021 acacgaccga ttcggcgatc cgcatcacgc atatcccgtc ggggctcgtc gtgatccagc
     1081 aggaccagcg cagccagcac aagaatcgcg ccaaggcgat gcaggtgctg cgcgcgcggc
     1141 tctatgacct cgagcgcgag aagatccaca gcgccgaggc gtcggcgcgc aagtcgatgg
     1201 tcggatcggg cgaccgctcc gaacgcatcc gcacctataa ttttccgcag gggcgcgtga
     1261 ccgaccaccg catcaacctg acgctctaca agctcgacga gatgatgcag gggctggtgc
     1321 tcgacgagat catcgatgca ctcacggccg agcatcaggc gaagcttctc gcggccgagg
     1381 gcatggcgtg aggcccgcgc gatgaaacca aagcgcccgt cccgcgttgt tgatgcatcg
     1441 acaccagcaa aggggggacc ctctcatgat caagatgttc tcgacgatag cggtcaccgg
     1501 gctgatggcg ctggcgatga gcggcgtctc gcaggctcag gagcgcacct tccgcaagat
     1561 gacgtcgggc cagccggcgg ctctgcaggg cgcgatgacg cagtcgcagg cgcgcaaggc
     1621 gtgccggacg gagatgcgcg gcacgcgcga aagcaaggcc tcgctgaaca agaagatgat
     1681 aacctgcgtc aatctgaaga tgcagggcaa ctgacctcca ttcggggagg tgcggaaccc
     1741 gaagcgggtt ctgcgggaag cgttcgaggg gagggcgccg tgccgaccgt cagggaggcg
     1801 cggcgccatc ttgcgcagag cctcctgcgc gcgggctcgg cgagccccg
//
LOCUS       DATMSU010000094         3278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_153497,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000094 DATMSU010000000
VERSION     DATMSU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153497"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..625)
                     /locus_tag="VN034_02505"
     CDS             complement(137..625)
                     /locus_tag="VN034_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWV59497.1"
                     /translation="MTGTPHLLIAWHSRTGGSRALAEAAAEGAAKAAKLVGADEVTPA
                     LLLEAGGYLFVGPENLAALSGAMKEMFDRCYYPCLGKLEGRPYATIICAGSDGENAQR
                     QLDRIATGWRLKRVAEPVIVNTAAQTPEAILAPKTIAPDRLAEARDMGAALAEGIAAG
                     IF"
     gene            689..2650
                     /locus_tag="VN034_02510"
     CDS             689..2650
                     /locus_tag="VN034_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HWV59498.1"
                     /translation="MRRLTALLLAAGLPFTVAAQDPASEATRAAQAELAKRLPLGDPR
                     DEANATRGKIAEIAGGLIQTADGRIVWDRRSYAFLDAKEAPDTVNPSLWRQARLNAVH
                     GLFEVVPDKIWQVRGYDISVMTIIRGKSGWIVIDPLLSEEAAAASWKLFADTVEAKSG
                     KRPIKAVIFSHSHSDHFGGVGGIVTPERVKAENIRIIAPHGFSEEATSENVLAGGAMG
                     RRALYMFGAILPPGPTGQVDTGLGPKLSSGTIGYMEPTETVGEKGGTLTIDGLVFDFL
                     DAGGTEAPSEFVFYIPAYKALHTTEIVTHNLHNILTLRGAQVRDALRWSKVIDAMLLK
                     WGGKAEVAMASHHWPTWGAGEVSVLLANQRDAYRYVHDRTLFLANRGATLHELADQTA
                     EAPVQAADFSTRGYYGTLNHDMKAVYQRYFGWWDGNPANFNPLPPEQSAPKYVALAGG
                     ADKLLAAGRAALKAGDYRWAAELLNKLVFAQPDNRDARAALASAYDQLGYQAESGAWR
                     NYYLAGAASLRGNAVEAATGNGQSRSFVSAIPTAVFFDALATRFDAAKGGAVKGIFQF
                     ILPDSKESVAVVVGGGVEFPRYGVTDPAPTATITIDRKILDEAMMGRAQFPALIQSGA
                     IRIDGDRAAFLSWFALHPPADPRFNVVVP"
     gene            2676..3206
                     /locus_tag="VN034_02515"
     CDS             2676..3206
                     /locus_tag="VN034_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWV59499.1"
                     /translation="MTDTPAAHPTDPFDAEALNAAEGLHPVDPAYAKVLRIATALNLI
                     PLAIGASIFDALLIRHIEGPYGLITALAWLIAIVTIVSFPSRRVSRWGYKIGEGQLRV
                     ARGWLFRTDTIVPFVRVQHIDVGQGPIERWFGLSHLIVHTSGTHNSTVTLPGLPSDLA
                     AAMRETIRRHIQTDFA"
BASE COUNT          542 a         1070 c         1139 g          527 t
ORIGIN      
        1 agccgccacc cgcgcgcggc gaaccagtcg gtgaaagggg cggggagcgg catggacgag
       61 atgtggcgcg ctgcacgcgc aggggcaagg gcttccgccc gtgccgaagc tgcgcgaccg
      121 gttgcgggcg ctggcttcaa aaaatcccgg ccgcaatccc ctccgcgagc gcggcgccca
      181 tgtcgcgggc ttcggcgagg cggtccggcg cgatggtctt cggcgcgagg atggcttcgg
      241 gggtctgcgc cgcggtattg acgatcaccg gctcggcgac gcgcttcagc cgccagccgg
      301 tggcgatacg gtcgagctgg cgctgggcat tctcgccgtc cgatcccgcg cagatgatcg
      361 tcgcataggg ccggccttcg agctttccga ggcagggata atagcagcgg tcgaacatct
      421 ccttcatcgc gcccgacagc gcggcgagat tttcggggcc gacgaagagg taaccgcccg
      481 cttccagcag aagcgccggc gtgacctcgt ccgcgccgac gagcttcgct gccttagcgg
      541 caccttcggc cgcggcttcg gcgagcgccc tgctgccacc ggtgcggctg tgccaggcga
      601 tgaggaggtg cggcgtgccg gtcatgtcgc gatgcttgcc aagcggagag gtgcggcgca
      661 agctgccccg gaatggggga gagagatgat gcgccgattg accgccctgc tgcttgccgc
      721 gggcctgccc tttaccgttg ccgcacagga cccggcgagc gaagcgaccc gcgccgcaca
      781 ggccgaactg gcgaagcggc tgccgctggg tgacccgcgc gacgaggcca atgcgacgcg
      841 cggcaagatc gccgagattg cgggcgggct gatccagacc gccgatggca gaatcgtgtg
      901 ggaccggcgg tcctacgcct tcctcgacgc gaaggaggcg cccgatacgg tcaatccttc
      961 gctatggcgg caggcgcggc tcaacgctgt gcacggattg ttcgaggtgg tgccggacaa
     1021 gatctggcag gtccgcggtt acgatatttc ggtgatgacg atcatccgcg gcaagagtgg
     1081 ctggatcgtc atcgatcccc tgctctccga agaagcggcg gcggcgagct ggaagctgtt
     1141 cgccgatacg gtcgaggcga aatcgggcaa gcggccgatc aaggcggtga tcttttcgca
     1201 cagccatagc gatcatttcg gcggcgtcgg cgggatcgtc acacccgagc gggtgaaggc
     1261 ggagaatatc cgcatcatcg cgccgcacgg attttcggag gaagcgacgt ccgaaaatgt
     1321 cctcgcgggc ggcgcgatgg ggcggcgcgc gctctatatg ttcggcgcca ttctgccgcc
     1381 gggaccgacg ggacaggtcg acaccgggct gggcccgaag ctgtcgtcgg gcacgatcgg
     1441 ctatatggag ccgaccgaga ccgtcggcga gaaaggcggc acgctgacga tcgacgggct
     1501 ggtcttcgac ttcctcgacg cgggcggcac cgaggcgccg tcggagttcg tcttctacat
     1561 ccccgcctac aaggcgctgc acacgaccga gatcgtgacc cacaatctcc acaatatatt
     1621 gacgctgcgc ggggcgcagg tgcgcgatgc gctgcgctgg tcgaaggtga tcgacgcgat
     1681 gctgctcaaa tggggcggca aggccgaggt cgcgatggcg tcgcatcatt ggccgacctg
     1741 gggggcgggc gaggtgtcgg tgctgctcgc gaaccagcgc gatgcctatc gttatgtcca
     1801 cgaccgcacg ctctttctcg cgaaccgcgg ggcgacgctc cacgaactcg ccgaccagac
     1861 cgccgaggcg ccggtgcagg cggccgattt ttcgacgcgc ggctattatg gcacgctcaa
     1921 ccacgacatg aaggcggtct accagcgcta tttcggttgg tgggatggca acccggcgaa
     1981 cttcaacccg ctgccgcccg agcaatcggc gcccaaatat gtcgcgctcg cgggcggcgc
     2041 cgacaagctg ctcgcggcgg gacgggcggc gctgaaggca ggcgattatc gctgggcggc
     2101 tgagcttttg aacaagctgg tctttgcgca gcccgacaac agggatgcgc gcgcggcgct
     2161 tgcttcggcc tatgaccagc tcggctatca ggccgaatcg ggcgcatggc gaaactatta
     2221 tctcgcaggg gcggcgagcc tgcgcggcaa cgcggtcgaa gcggcgacgg gcaacgggca
     2281 aagccgcagc ttcgtcagcg cgatcccgac cgcggtcttc ttcgatgcgc tcgcgacgcg
     2341 cttcgacgcc gcgaagggcg gcgcggtcaa ggggattttc cagttcatcc tgcccgacag
     2401 caaggaaagc gttgccgtcg tcgtcggcgg cggggtcgaa tttccgcgct atggcgtgac
     2461 cgaccccgct cccaccgcga cgatcacgat cgaccgcaag atcctcgatg aagcgatgat
     2521 gggacgcgcg cagtttcccg cgctgatcca gtcgggggcg atccgcatcg acggcgatcg
     2581 cgcggctttc ctgtcgtggt tcgcgctcca cccgcccgcc gacccgcgct tcaatgtggt
     2641 tgtgccatag tcatataaga cgctaacgga ggatcatgac cgacacgccc gccgcgcatc
     2701 caaccgaccc cttcgacgcc gaggcgttga acgccgccga ggggcttcac ccggtcgatc
     2761 cggcctatgc gaaagtgttg cgcatcgcga ccgcgctcaa cctgatcccg ctcgcgatcg
     2821 gtgcgagcat attcgacgcg ctgttgatcc gtcatatcga gggcccctac ggcctgatca
     2881 cggcgctggc gtggctgatc gcgatcgtca cgatcgtcag cttcccgtcg cggcgcgtgt
     2941 cgcgctgggg ctacaagatc ggcgaaggac agctccgcgt agcgcgcggg tggctgttcc
     3001 gcaccgatac gatcgtgccc ttcgtgcgcg tccagcatat cgacgtcggg caggggccga
     3061 tcgaacgctg gttcggcctg tcgcacctga tcgtccacac gtcgggcacg cacaacagca
     3121 cggtcacgct gcccggattg cccagcgacc tcgcggcggc gatgcgcgag acgatccgcc
     3181 gccatatcca gaccgacttc gcatgaaggg cgccgccgcc cttgtcgcgc cggagggcga
     3241 gacgcgctgg cagcggctgc accccgcaac gctggcgc
//
LOCUS       DATMSU010000095         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172483,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000095 DATMSU010000000
VERSION     DATMSU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172483"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VN034_02520"
     CDS             complement(<1..784)
                     /locus_tag="VN034_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV59500.1"
                     /translation="MAGLSIERARKSFGATEVLKDVSIEVADGEFTVIVGPSGCGKST
                     LLRSVAGLEELTNGRIRIGDRDVTGLAPSERGIAMVFQSYALYPHLTVYENMAFGLRI
                     AKADKAAIDAAVRRAAAILNIEALLDRKPAELSGGQRQRVAIGRAIVRQPQIFLFDEP
                     LSNLDADLRVRMRYEFAELHRQLGTTTLYVTHDQVEAMTLADRIIVMRDGRIEQVGTP
                     RELYDRPANIFVAQFLGTPRMNILSATMAEGGRATLADGRDVA"
     gene            complement(807..2171)
                     /locus_tag="VN034_02525"
     CDS             complement(807..2171)
                     /locus_tag="VN034_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HWV59501.1"
                     /translation="MNLSSQATVHGGGRSNAMAAFAAVTVLFFAWGFITSLIDPLVAA
                     VKGIFTLSDAEAQLSASAFFIAYGLMSFPAAALIARFHSVPSILTALSTMVAGCLVML
                     AAANLAVYPLVLAGLFILASGITILQVAANPLAAALGDPKRSHFRLTFSQTFNSFGTF
                     LGPLIGAHLFLEGVEVKEGTVVTEAVRAQALGGIDAAYFWICGLIIALLFFFWFSRRI
                     VNEAVPPAPIGERAGMGELIREAFSSRWALLGGLAIFLYVGAEVAIGTQMALFLNSDA
                     IWGQSDAPFGTFGWIMGNDGVRGVSLQEAGKAVALYWGGAMVGRAIGSVLLARFSASK
                     LLVIFTAIAALLCLYIVFVGGVSAGFVALSIGLFNSIMFPVIFTLTLERSTARAEATS
                     GLLCTAIVGGAAIPYLVGQLSDAVGYSAALVLPAACYVALCVFAIAAGRAPARQAGAG
                     VTVH"
     gene            2647..4104
                     /gene="guaB"
                     /locus_tag="VN034_02530"
     CDS             2647..4104
                     /gene="guaB"
                     /locus_tag="VN034_02530"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWV59502.1"
                     /translation="MEIITGLTFDDVLLVPGASDILPSDANLATQLTSEISLNIPVLS
                     SAMDTVTEADMAILMAQIGGIGVLHRNLTVEEQAAAVRQVKRFESGMIVNPITITPDA
                     PLSYATALMDQHRISGIPVVETGGKLVGILTHRDVRFADNPGQPVRELMTAENLATVR
                     AGVGQDEARKLLHQRRIEKLLVVDDEYHCIGLITVKDMEKAVNFPDATKDGNGRLRVA
                     AATNTGDSGVERAEALLEAECDLIVVDTAHGHSKGVGETVARIKKLSNRVQVLAGNVA
                     TGDATKALIDAGADGVKVGIGPGSICTTRIVAGVGVPQLTAILDSVEAASKLGVPVIA
                     DGGLRTSGDVAKALAAGASSVMVGSMLAGTSEAPGETFLYQGRTYKSYRGMGSVGAMA
                     RGSADRYFQQDIKDQMKLVPEGIEGQVPFKGPAKDVIHQMVGGVKAAMGYTGSRTLKD
                     FRERAKFVRITNAGLRESHVHDVAITREAPNYPAG"
BASE COUNT          801 a         1397 c         1376 g          608 t
ORIGIN      
        1 gggcgacgtc gcggccgtcg gccagcgtcg cgcgcccgcc ttcggccatc gtcgcggaca
       61 ggatgttcat ccgcggcgtc ccgagaaact gcgcgacgaa gatattcgcg ggccggtcat
      121 aaagttcgcg cggggtgccg acctgttcga tccgcccgtc gcgcatcacg atgatccggt
      181 cggcgagcgt catcgcttcg acctgatcgt gcgtcacata gagcgtcgtc gtgccgagct
      241 gacggtgaag ctcggcaaat tcatagcgca tccgcacgcg caaatcggcg tcgaggttcg
      301 acagcggctc gtcgaacaga aaaatctgcg gttggcggac gatcgcgcgg ccgatcgcga
      361 cgcgctgccg ctgcccaccc gacagttccg ccggtttgcg gtccagcagc gcctcgatgt
      421 tcaatatcgc cgccgcgcgg cgcaccgcgg catcgatagc agccttgtcg gccttcgcga
      481 tgcgcagccc gaacgccata ttctcataga cggtcaggtg cggatagagc gcataggact
      541 ggaacaccat cgcgatcccg cgttccgacg gcgcgaggcc ggtaacgtcg cggtcgccga
      601 tgcggatacg gccgttcgtc agctcctcaa gccctgcgac cgaccgcaaa agcgtcgatt
      661 taccgcatcc cgacggcccg acgatcacgg tgaattcgcc gtcggcgacc tcgatcgaca
      721 cgtccttcag gacctcggtc gcgccgaagc tcttgcgcgc gcgctcgatc gacaatccag
      781 ccatgatccc ccgcgttccc ggcggctcag tggaccgtca cgcccgcgcc cgcctgccgc
      841 gcgggtgcgc gcccggcggc gatggcaaag acgcacagcg cgacatagca ggcggcgggc
      901 aggaccagcg cggcgctgta accgaccgcg tccgacaatt gtccgacgag ataggggatc
      961 gccgcgccgc cgacgatcgc ggtgcacagc agccccgatg tcgcctcggc gcgcgcggtc
     1021 gaacgttcga gcgtcagcgt gaagatcacc gggaacatga tcgaattgaa caggccgatc
     1081 gacagcgcga cgaagcccgc gctcaccccg ccgacgaaca cgatataaag gcagagcagc
     1141 gccgcgatgg cggtgaagat gacgagcagc ttcgatgccg agaaacgcgc gagcagcacc
     1201 gatccgatcg cgcggccgac catcgcgccg ccccagtaaa gcgcgaccgc tttgcccgct
     1261 tcctgcagcg acacgccgcg aacgccgtcg ttgcccatga tccagccgaa tgtgccgaag
     1321 ggcgcgtcgg actgacccca gatcgcatcg ctgttgagga acagcgccat ctgggttccg
     1381 atggcaacct cggcgccgac atagagaaag atcgcgagcc cgccgagcag cgcccagcgc
     1441 gacgaaaagg cctcgcggat cagctcgccc atcccggcgc gctcgccgat cggcgcgggc
     1501 ggcaccgctt cgttgacgat ccggcggctg aaccagaaaa agaagagcag cgcgataatg
     1561 aggccgcaga tccagaaata ggccgcgtcg atcccgccga gcgcctgcgc gcgcaccgcc
     1621 tcggtcacca ccgtgccttc cttgacctcg acgccttcga ggaacaggtg cgcgccgatc
     1681 agcgggccga ggaaggtgcc gaagctgttg aaggtctggc tgaaggtcag gcggaaatgg
     1741 ctgcgtttcg gatcgccaag cgccgccgcg agcgggttcg ccgcgacctg caggatggtg
     1801 atgccgctcg cgaggatgaa caggcccgcg agcaccagcg gatagacggc gaggttcgcc
     1861 gccgcgagca tgacgaggca gccggcgacc atcgtcgaca gcgcggtcag gatcgacggc
     1921 accgaatgaa agcgcgcgat caacgccgcg gcggggaagg acatcaggcc ataggcgatg
     1981 aaaaaggccg acgccgacaa ttgcgcctcg gcgtcgctca gcgtgaagat gcccttcacc
     2041 gcggcgacca gcgggtcgat cagcgaggtg atgaagcccc aggcgaaaaa cagcacggtc
     2101 accgcggcaa aggccgccat ggcattcgac cggccgccgc cgtggacggt cgcctggctg
     2161 gacaggttca ttcggaatat cctcttcgcc gttactatgt tatgcggcgg gtctaacggg
     2221 gcctcggaag caatgcaacc ggttacaccg gcgtcgatgg cgtgcgacct cattatcctt
     2281 ccccctcgat gggggaagga tacgcagcct tatcgcgaag cgattaggcg gagttggatg
     2341 ggggtgttgg cgcgttcttg cacaatcgct tcaggccgag acctcacccc caccgctgcg
     2401 actagcgaac aagttcgcaa gtctcgctgc ctcccccatc aagggggagg agattaggga
     2461 gatcggatgg acgtaattcg gccttctgat ccgccaagtc cgccctcccg aaacgtacga
     2521 aataatcgtt cgtcccccac tggcgcgcgc gggcgcgact ggttacatgg gccgccacca
     2581 aatgattcgc gcgtgccgcc ccgaaagctt gggggcaggc gccgcctgca ccagaaagtg
     2641 gcacccatgg aaatcatcac cggcctgacc ttcgacgacg tgctgctggt gcccggcgcg
     2701 tcggacatcc tgccgtcgga cgcgaacctc gcgacccagc tgaccagcga gatcagcctc
     2761 aacatcccgg tcctgtcgtc ggcgatggac acggtgacag aggccgacat ggcgatattg
     2821 atggcgcaga tcggcggcat cggcgtgctt caccgcaacc tgaccgtcga ggaacaggcc
     2881 gccgcggtgc ggcaggtcaa gcgtttcgaa agcggcatga tcgtcaaccc gatcaccatc
     2941 acccccgacg cgccgctctc ctacgcgacc gcgctgatgg accagcaccg gatttcgggc
     3001 attccggtgg tcgaaacggg cggtaagctc gtcggcatcc tgacccaccg cgacgtgcgc
     3061 ttcgccgaca atccgggcca gccggtgcgc gagttgatga ccgccgaaaa tctcgcgacg
     3121 gtgcgcgccg gggtcgggca ggacgaagcg cgcaaattgc ttcaccagcg ccggatcgaa
     3181 aaattgctcg tcgtcgacga cgaatatcac tgcatcggcc tgatcaccgt caaggacatg
     3241 gaaaaggcgg tcaatttccc cgacgcgacc aaggacggga acggccgcct gcgcgtcgcg
     3301 gcggcgacga ataccggcga tagcggtgtc gagcgcgccg aggcgctgct cgaggccgaa
     3361 tgcgatctga tcgtcgtcga taccgcgcac ggccatagca agggcgtcgg cgagaccgtc
     3421 gcacgcatca agaaactgtc gaaccgcgtg caggtgctcg cgggcaatgt cgcgaccggc
     3481 gacgcgacca aggcgctgat cgatgcgggc gccgacggcg tcaaggtcgg cattggcccg
     3541 ggctcgatct gcaccacccg gatcgtcgcg ggcgtcggcg tgccgcaact caccgcgatc
     3601 ctcgacagcg tcgaagccgc ctcgaaactc ggcgtgccgg tgatcgccga cggcggcctc
     3661 cgcacctcgg gcgatgtcgc caaggcgctg gcggcgggcg cttcgagcgt gatggtcgga
     3721 tcgatgctcg cgggtaccag cgaagcgccc ggcgaaacct tcctctatca ggggcgcacc
     3781 tataaaagct atcgcggcat gggcagcgtc ggcgcgatgg cgcggggctc ggccgaccgc
     3841 tatttccagc aggatatcaa ggaccagatg aagctggttc ccgagggcat cgaggggcag
     3901 gtgccgttca agggccccgc gaaggacgtc atccaccaga tggtcggcgg cgtgaaggcg
     3961 gcgatgggct ataccggcag ccgcacgctc aaggatttcc gcgagcgcgc caaattcgtg
     4021 cgcatcacca acgcgggcct tcgcgaaagc catgtccacg acgtcgcgat cacgcgcgag
     4081 gcgccgaact atccggcggg ctgaggccgg ccggtgacgc ccgcagcgcg catccaggca
     4141 gcgatcgaga ttctcgacgc catcgccgcc gccgcgcgcg ag
//
LOCUS       DATMSU010000096         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_106194,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000096 DATMSU010000000
VERSION     DATMSU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106194"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..759)
                     /locus_tag="VN034_02535"
     CDS             complement(97..759)
                     /locus_tag="VN034_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59503.1"
                     /translation="MASLALPPALAAMSNADRIRDTALSETLLGQFTPASGDERLIAR
                     YNKMSAEQRGNFSFTPALTDRARQNRAITVVIRARDDASSAARTSALAAGRTAPVAIA
                     PVKYNLGASVGFEKFVTPSLPRGTDLRNLPVAKAPEQDEKKSRFATRMVNRPSDPSGA
                     TDRVTAPGEASAVDVVSSYRLTKNIDVTAGVRYRSDDRVEPLTDSRRDSQAVYIGTAF
                     RF"
     gene            1093..1566
                     /locus_tag="VN034_02540"
     CDS             1093..1566
                     /locus_tag="VN034_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HWV59504.1"
                     /translation="MSQLSVPAKLGFTIGRRPATLALLGLAALGLSACANKERPRADL
                     AASQVTTIGVNSYLWRASLDALSFMPLLQADSAGGVVITDWYANPSNPGERMKVTVSI
                     LDQDLRADALRVAASRQVSQGGAWVDAPVQAATVQKLEEIILTRARDLRRSAIRD"
     gene            1618..4179
                     /gene="leuS"
                     /locus_tag="VN034_02545"
     CDS             1618..4179
                     /gene="leuS"
                     /locus_tag="VN034_02545"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWV59505.1"
                     /translation="MTRETRFGALAADARWQKAWEAANSFATTDSDAKDSGRPKAYIL
                     EMFPYPSGRIHMGHVRNYAMGDVLARFKRMTGHDVLHPMGWDAFGMPAENAAMEKGVH
                     PGGWTRDNIASMRGQLKRLGLAIDWSRELATCEPDYYGQEQALFLDLFAAGLVTRKHS
                     YVNWDPVDMTVLANEQVIDGRGWRSGALVEKKKLSQWFLKITDFADELLEGLDSLDKW
                     PDKVRLMQENWIGKSQGLEFSFKLAGGAVGFDVFTTRPDTLFGASFAAISPDHPLAEK
                     LAKDSPELAAFIEECRRQGTAAEQIDTAEKMGFDTGIAVEHPLDPDWHLPVWVVNYVL
                     MDYGTGAIFGCPAHDQRDLDFAHKYELPVYRVIADGDEVAQHFTGTEAYTGPGRLVNS
                     HFLDGMSIDEAKAAVIARAEHEGWGKGTTVWRLRDWGVSRQRYWGTPIPFVHCAACGM
                     VPVPKSQLPVVLPEDADFSVPGNPLDRHPTWKHVPCPSCGGEAVRETDTLDTFVDSSW
                     YFLRFASAPSDKPFDPEVIRRWLPVDQYIGGIEHAILHLLYARFWTRALKHMGMIDIA
                     EPFASLFTQGMVTHETYSRSQGEGLPPLFFTPDEVERGAEGATLTADGAPVDIGRVIK
                     MSKSKKNVVDPDAILDQYGADAARWFMLSDSPPERDLPWSEAGIEGAWRFVQRLWRIF
                     GDTENIGDGGEDLGLARKLHQAIAGVAADIESLGFNKAVAKIHALANEIEKAKPSATR
                     AEASRTLILLVAPMMPHLAEEAWAALPASQRTTPMIADAAWPAADPALLVDDEVTIAI
                     QMAGKLRDTMTIAKGLDKDATEAAALARPRIVELLAGATPKKVIVVPDRLVNIVP"
     gene            4312..>4865
                     /gene="lptE"
                     /locus_tag="VN034_02550"
     CDS             4312..>4865
                     /gene="lptE"
                     /locus_tag="VN034_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWV59506.1"
                     /translation="MRSLLVSALTALSLTIGGCGLRPLYANGSKGAVAQILADVDVAP
                     IEGHEGWLVRNALRDRLEATQGGEGAGKRLRLDVRLEDSITGFGVRADDTVTRERRTL
                     RARYQLVDSASGAVLLDATAAQDAGIDVVGSEYATIAAESTALERLASGVADQIVARP
                     FAAAHLRTSLIAASFASAPELHRNT"
BASE COUNT          835 a         1617 c         1602 g          811 t
ORIGIN      
        1 ccgcgacccg gagcgcagcg cacttaatgt gcgtgagcac cggaagcgcg gaaggtcgcc
       61 atttgcagac cgccctgatg caaatagcga tgcagattag aagcggaacg cggttccgat
      121 atagaccgcc tggctgtcgc gacgcgaatc ggtgagcggt tcgacgcggt cgtcgctccg
      181 gtaccggacg cctgcggtca catcgatatt cttggtcagt cgatagctgc tgacgacatc
      241 gacggcggac gcctcgccgg gagcggtgac gcgatcggtt gccccgctcg ggtcgcttgg
      301 gcggttcacc atacgcgtgg cgaagcgtga cttcttctcg tcctgttcgg gcgccttggc
      361 gaccggcagg ttgcggagat cggtgccgcg cggcagcgac ggcgtgacga atttttcgaa
      421 gccgaccgac gcgccgagat tatatttgac cggcgcgatc gcgaccggcg cggtgcggcc
      481 cgccgccaga gccgacgtgc gcgcggcgct cgacgcatcg tcgcgggcgc gaatcacgac
      541 ggtgatcgcg cggttctgac gggccctgtc ggtcagggcg ggcgtgaagc tgaagttgcc
      601 gcgctgttcg gccgacatct tgttatagcg cgcgatcagc ctttcgtcgc ccgacgccgg
      661 ggtgaactga cccagcaggg tttccgaaag cgcggtgtcg cgaatgcggt cggcgttcga
      721 catcgcggca agggccggcg gcagcgcaag cgaagccacc gccaaagcgg tgagacctgc
      781 tttccaaaaa tgtcgcgatg tccgcgccat tgctctgcga ctccaacccc aatcgattca
      841 tcaaatagga ccgccggccc gagaatgcca gtgaatcccg ccaaattgtt gcaacgattc
      901 cgctttttgc caaaattgtt gcgcgatcgt cacagtgcgc atcgaatctg accagaatct
      961 gaatggcgcc gcggccgaga ttgccgcgct tgcagggtga cggcaacggc gatagagacg
     1021 ctggcaattc cgttatgcgc ggccggtttt cttctattcg ggcgggctca aagagataag
     1081 gtgttaatcg gcatgtccca gctttccgtc cccgccaagc tcggcttcac gatcggtcgc
     1141 cgcccggcca cgctcgcgct gctcggactt gcggcgcttg gcctttcggc atgcgcgaac
     1201 aaggaacggc cgcgcgccga tctcgccgcc agccaggtga cgacgatcgg cgtgaacagc
     1261 tatctgtggc gcgcctcgct cgacgcgctg tcgttcatgc cgctgcttca ggccgacagc
     1321 gcgggcggcg tcgtgatcac cgactggtac gcgaatccgt cgaacccggg cgagcggatg
     1381 aaggtcaccg tgtcgatcct cgaccaggat ttgcgcgccg acgcgctgcg cgtcgcggcg
     1441 tcgcggcagg tgtcgcaggg cggcgcctgg gtcgatgccc cggtgcaggc ggccaccgtg
     1501 cagaagctcg aggaaatcat cctcacccgc gcccgcgacc tgcgccgcag cgccatcagg
     1561 gactaatccg gcggcgcgcg cctgcgcgcc catagatact aacggggtaa ccgacgaatg
     1621 acccgcgaaa cgcgctttgg cgccctcgcc gccgacgccc gctggcaaaa ggcgtgggag
     1681 gcggcgaaca gctttgccac gaccgacagc gacgccaagg attctggcag gcccaaggcc
     1741 tatatcctcg aaatgttccc ctacccctcg gggcgcatcc acatggggca tgtgcgcaac
     1801 tatgcgatgg gcgacgtgct cgcgcgcttc aagcggatga ccgggcacga cgtgctccac
     1861 ccgatgggct gggacgcgtt cggcatgccg gccgaaaatg ccgcgatgga aaagggcgtc
     1921 caccccggcg gctggacgcg cgacaatatc gcgtcgatgc gcggccagtt gaaacgtttg
     1981 gggctggcga tcgattggag ccgcgagctc gcgacgtgcg agcccgatta ttacgggcag
     2041 gaacaggcgc tgttcctcga cctgttcgcc gcggggctcg tcacgcgcaa gcacagctac
     2101 gtcaactggg acccggtcga catgaccgtg ctcgcgaacg agcaggtgat cgacggccgc
     2161 ggctggcgct ccggcgcgct ggtcgagaag aagaaactgt cgcagtggtt tctcaagatc
     2221 accgacttcg ccgacgagct gctcgaaggt ttggacagcc tcgacaaatg gcccgacaag
     2281 gtccggctga tgcaggaaaa ctggatcggc aagtcgcagg ggctcgagtt cagcttcaag
     2341 ctcgccggcg gcgcggtcgg tttcgacgtc ttcacgacgc gtcccgacac gctgttcggc
     2401 gcgagcttcg cggcgatctc gcccgaccat ccgcttgccg agaagctcgc caaggattcg
     2461 cccgaactcg ccgcgttcat cgaggaatgc cggcgtcagg gcaccgccgc cgagcagatc
     2521 gacaccgccg agaagatggg tttcgacacc ggtatcgccg tcgagcatcc gctcgacccc
     2581 gactggcacc tgcccgtctg ggtcgtgaat tatgtgctga tggactatgg caccggcgcg
     2641 atcttcggct gccccgcgca cgaccagcgc gacctcgact tcgcgcataa atatgagctt
     2701 cccgtctatc gcgtgatcgc cgacggcgac gaggtcgcgc agcatttcac cgggaccgaa
     2761 gcctacaccg gcccgggccg cctcgtgaac agccacttcc tcgacggcat gagcatcgac
     2821 gaggcgaagg ccgccgtcat cgcgcgcgcc gagcatgaag gctggggcaa gggcacgacc
     2881 gtgtggcgcc tgcgcgactg gggcgtgtcg cgccagcgtt actggggtac gccgatcccc
     2941 ttcgtccatt gcgccgcatg cggcatggtg cccgttccca aaagccagct ccccgtcgtg
     3001 ctgcccgagg atgccgattt ctcggtcccc ggcaatccgc tcgaccgcca tccgacgtgg
     3061 aagcatgtcc catgcccgtc gtgcggcggc gaagcggtgc gcgagaccga cacgctcgac
     3121 actttcgtcg attccagctg gtatttcctg cgcttcgcat cggcgccgag cgacaagccc
     3181 ttcgaccccg aagtgatccg ccgctggctg cccgtcgatc aatatatcgg cggcatcgag
     3241 catgcgatcc tccacctcct ctacgcgcgc ttctggacgc gcgcgctcaa acatatgggg
     3301 atgatcgaca tcgccgagcc cttcgccagc ctgttcacgc agggcatggt gacgcacgag
     3361 acctatagcc gcagccaggg cgagggcttg ccgccgctct tctttacccc cgacgaggtc
     3421 gaacgcggcg ccgagggcgc gacgctgacc gccgacggcg cgccggtcga catcggccgc
     3481 gtgatcaaaa tgtcgaagtc gaagaagaat gtcgtcgatc ccgacgcgat cctcgaccag
     3541 tacggcgccg acgccgcacg ctggttcatg ctttccgaca gcccgcccga gcgcgatctg
     3601 ccgtggagcg aggcgggcat cgagggcgcg tggcgcttcg tccagcgcct gtggcggatt
     3661 ttcggggaca ccgagaacat cggcgacggc ggcgaagact tgggcctcgc gcgcaagctg
     3721 catcaggcga tcgccggcgt cgcggccgac atcgaatcgc tgggcttcaa caaggcggtc
     3781 gcgaaaatcc atgcgctcgc gaacgagatc gagaaggcga agccttcggc gacgcgtgcc
     3841 gaggcgtccc gcacgctgat cctgctcgtt gcgccgatga tgccgcacct cgccgaagag
     3901 gcttgggcgg cgctgccggc aagccagcgc acgacaccga tgatcgccga cgccgcgtgg
     3961 cccgccgccg atccggcact gctcgtcgat gacgaggtga caatcgcgat ccagatggcg
     4021 ggtaagctgc gcgatacgat gacgatcgcc aagggtctcg acaaggacgc caccgaagcg
     4081 gcggcgctcg cccgcccgcg tatcgtcgag ctgctcgccg gcgcgacgcc gaagaaggtg
     4141 attgtggtgc ccgaccgttt ggtgaatatc gttccataga agatggcttc cgccgcgcct
     4201 ccctactccg ctcgcatcga gcgaagtcga gatgcccatc gttcgtgcgt gccttcggtg
     4261 tgtctcgact tcgctcgaca cgaacgggga taagggtcaa tagtgttcaa gatgcgtagc
     4321 ctcctcgtct ctgcccttac cgccctttca ctgacaatcg gcgggtgcgg cctgcgtccc
     4381 ttgtacgcga acggcagcaa gggcgcggtg gcgcagatac tcgccgatgt cgacgtcgcg
     4441 ccgatcgagg gacatgaagg ctggctcgtc cgcaacgcgc tccgcgaccg gttggaggcg
     4501 acgcagggcg gcgagggcgc gggcaagcgc ctccgcctcg acgtccggct cgaggattcg
     4561 atcaccggct tcggcgtccg cgccgacgat acggtgacgc gcgagcgtcg gacgctgcgc
     4621 gcgcgctatc agctcgtcga ttcggcatcg ggcgcggtgc tgctcgacgc gaccgccgcg
     4681 caggatgccg ggatcgatgt cgtcggcagc gaatatgcga cgatcgccgc cgaatcgacc
     4741 gcgctcgaac ggctcgcttc gggcgtcgcc gaccagatcg tcgcgcgccc gttcgctgcc
     4801 gcccacttgc gcaccagctt gatcgccgcc tcgttcgcct cggcgccgga gttgcacagg
     4861 aacac
//
LOCUS       DATMSU010000097         7569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_1989, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000097 DATMSU010000000
VERSION     DATMSU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /gene="pnuC"
                     /locus_tag="VN034_02555"
     CDS             <1..600
                     /gene="pnuC"
                     /locus_tag="VN034_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HWV59507.1"
                     /translation="VDASAPRTVRMSAIEGLAAALVLVNVALVALRSVWNYPFALVAV
                     TLYAFVFFEAKLYSDMLLQGFFFALNLYGWAAWTRARDESGVPVRWMTDRARRGWALA
                     TIAAWAGWSFAMDRYTDAVAPWIDGAIAMLSITAQWLLARRRVESWFLWILVDLIAVP
                     LFASRGLYATSAVYVVLLGLSIDGLIQWRRAAATDKMAL"
     gene            597..1115
                     /locus_tag="VN034_02560"
     CDS             597..1115
                     /locus_tag="VN034_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV59508.1"
                     /translation="MTRHICLHGAESTGKSTLAPRLALRLGGLVVPEYGRTYAEANGT
                     DLDEVDLLAIFDGHRAATEAALAQKPEWLVSDTDPLMTQAWAIMLLGRRLPEIDHWDA
                     VADLYLVPAMDLPWQEDGTRLFGSELARAQFMDIAIGELDRRGLSWAWVEGEGDARVE
                     NALAAIEAAGLG"
     gene            1368..2957
                     /gene="lnt"
                     /locus_tag="VN034_02565"
     CDS             1368..2957
                     /gene="lnt"
                     /locus_tag="VN034_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWV59509.1"
                     /translation="MTALSLRIAAFADRRPKLLALLLGAVSATGFAPFHLWPLTLLAL
                     AGWMALVARSPKGSRALGIGWAFGVGHFVVGLNWIATAFTYQAAMPAWLGWIAVVLLA
                     LYLAVYPALTAWGAWLVKPKGDTHFAAPSYLLAFGGLWILTEWLRSWIFTGFAWNPMG
                     VIAVPHAAMPARWIGTYGMSGIVFLLAGVLILAWFRRRVAAAALFGSVLVFWGAAFVS
                     QMGSSADAAVRSKGIARIPVTVVQPNVGQEDKWEGSKADANFAKLARLTTPKDDIPRL
                     ILWPEAAVPDYLERGYPSVYYDRSPAEARARIVRLMNPNDVMLLGALKLELDKAGDVV
                     GARNAVMTVHADGTLGPRYDKAHLVPYGEYLPMRPILSAIGLSRLAPGDIDFWPGPGP
                     RTLDLGAFGRAGLQICYEIIFSGQVVDRDHRPDFIFNPSNDAWFGSWGPPQHLAQARL
                     RAIEEGLPVIRATPTGISAVIDADGRIVESLPMHKAGRIDTVVPAARAPTLFARFGNA
                     LPVGFALLLLALAIAFRRSGR"
     gene            3010..4230
                     /gene="metK"
                     /locus_tag="VN034_02570"
     CDS             3010..4230
                     /gene="metK"
                     /locus_tag="VN034_02570"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWV59510.1"
                     /translation="MRNSFLFTSESVSEGHPDKVADQISDSIVDLFLSKDPEARVACE
                     TLTTTQLVVLAGEIRCKGVFENDAWAPGALDEIEATVRNTVREIGYEQSGFHWQSFRF
                     ENNLHGQSAHIAQGVDESGDKDEGAGDQGIMFGYASDETPDLMPATLDYSHKILERMA
                     ADRKAGTAPFLEPDTKSQVTLRYANERPVEATAIVVSTQHAPGYFFHNGEGDEAKYTE
                     LRKYVLGVIADVLPAELLTANTVYHINPTGRFEIGGPDGDAGLTGRKIIVDTYGGASP
                     HGGGAFSGKDPTKVDRSAAYITRYLAKNIVAAGLARRCTIQLSYAIGVAEPLSIYVDL
                     HGTGTVEEGRIEAAIPQLVRLTPKGIRTHLGLNKPIYRQTAAYGHFGRTAEGDAFPWE
                     RTDLVDKLKAALAA"
     gene            4283..4996
                     /locus_tag="VN034_02575"
     CDS             4283..4996
                     /locus_tag="VN034_02575"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HWV59511.1"
                     /translation="MTAHKPGDPTTLNRLYGRSKGKPLRAGQQDLVDTLLPQIAVPEV
                     GEVSAERLFGQACPLHFEIGFGGGEHMAGRADMLPDHGFIGAEPFINGVAQALVHVAG
                     DHGERLPLGNVRIHHGDALEVLRRIPDGALSFAYLLHPDPWPKARHAKRRMMNDGPLD
                     LIAAKLKPGGEFRFGTDHPIYLQHALMVMRRHRHQYEWLAKDARDFQQRPGGWPETRY
                     EAKARAQGHEVWYFRYKRI"
     gene            complement(4993..5130)
                     /locus_tag="VN034_02580"
     CDS             complement(4993..5130)
                     /locus_tag="VN034_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59512.1"
                     /translation="MTRKKPRRETPRAAKPAVPLCRYEELAMRENRLRQAKAEKSEPR
                     D"
     gene            complement(5260..5595)
                     /locus_tag="VN034_02585"
     CDS             complement(5260..5595)
                     /locus_tag="VN034_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59513.1"
                     /translation="MSYQLSSFRRFPTAFAVVAASALAFTGLMASTTPAYAQSGGDYR
                     CAGLAEQAHVAASGVEGSKQSSAKRFVSTGKKLCEAGNERAAAKQFRAALKIAGVAEV
                     ETDSQMASR"
     gene            5679..6605
                     /locus_tag="VN034_02590"
     CDS             5679..6605
                     /locus_tag="VN034_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV59514.1"
                     /translation="MKQDLTIPQGALDGIEAFLRVAERRSFSAAAADLGVSPSAISQT
                     VKALEARVGAPLFMRTTRSVGLTQAGEMFLERAAPAYTGLADAYEAARNLGNRPAGRL
                     RINLMRGAVQPLFEPIIAGFCETYPDIELEIYADDALADLSAGGFDAGVRMGESLDAD
                     VIAVRLTGPFRFVVAAAPAYLAKHGRPETPEELRDHRCIRFRLATGALMPWTFEKGNR
                     EYDVSVTGPVIVNDWIAALVAIRAGVAMGMMAEPMAKAMVENGDLELVLTDYASATSG
                     LFLYYPGRKQVMPKLRAFIDYVREYLPDEITA"
     gene            6728..7498
                     /locus_tag="VN034_02595"
     CDS             6728..7498
                     /locus_tag="VN034_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWV59515.1"
                     /translation="MIDLAAIDFTALLPFILIGFAAQLVDGALGMAFGVICNTLLVAV
                     LGVPPATASARIHVVEIFTTGVSGLSHVFHRNIDWPLFFRLLIPGVIGGVLGAYVLSS
                     LHAEVVKPFVLGYLVLIGIWLLIRGVLYPPKFSKPKVVAPLAVVGGFLDAAGGGGWGP
                     VVTSNLLVQGCEPRKVVGTVNSVEFFLAVAVSAAFIWHLGIGEILGPTLGLIIGGVVA
                     APIGAMMAKRFSPKVMLMLVGTVLTATSAFGLYKVLVG"
BASE COUNT         1174 a         2507 c         2531 g         1357 t
ORIGIN      
        1 gtggacgcgt ccgcgccgcg aaccgtgcgc atgagcgcga tcgaggggct cgcggcggcg
       61 ctggtcctcg tcaacgtcgc gctcgtcgcg ctgcgcagcg tgtggaacta tcccttcgcg
      121 ctcgtcgcgg tgacgctcta cgccttcgtc ttcttcgagg cgaagctgta cagcgacatg
      181 ctgctccagg gctttttctt cgcgctcaac ctttacggct gggcggcgtg gacgcgtgcg
      241 cgcgacgaga gcggtgtacc cgttcgctgg atgaccgatc gcgcccgccg gggctgggcg
      301 ctggcgacga tcgcggcgtg ggcgggctgg agctttgcga tggaccgcta taccgacgcc
      361 gtcgcaccat ggatcgacgg cgcgatcgcg atgctcagca tcaccgcgca atggctgctc
      421 gcgcggcgcc gcgtcgaaag ctggttcctg tggatcctcg tcgatctgat cgcggtgccg
      481 ctcttcgcct cgcgcgggct ctacgcgacg agcgcggtct atgtcgtgct gctcggcctg
      541 tcgatcgacg ggctgatcca gtggcgccgc gccgccgcga cggacaagat ggcgctgtga
      601 cgcgccatat ctgcctgcac ggcgccgaaa gcaccggcaa gtcgacgctc gccccacgcc
      661 tcgcactgcg gctcggcggc ctcgtcgtcc ccgaatatgg ccgcacctat gccgaggcca
      721 acggcaccga cctcgacgag gtcgatctgc tcgcgatctt cgatggccac cgcgcggcga
      781 ccgaggcggc gctcgcgcag aagcccgaat ggctcgtatc ggacaccgat ccgctgatga
      841 cgcaggcgtg ggcgatcatg ctgctcggcc gccgcctgcc cgaaatcgac cattgggacg
      901 cggtcgccga cctctatctc gtccccgcga tggatctgcc gtggcaggag gacggcacgc
      961 gcctgttcgg cagcgaactc gcgcgcgcgc aattcatgga catcgcgatc ggcgaactcg
     1021 accggcgcgg gctttcatgg gcgtgggtcg agggcgaagg cgatgcgcgg gtcgagaatg
     1081 cgctggcggc gattgaggcg gcggggttgg ggtgaggagt tgcgggcgcg gtgtggatgt
     1141 ccgctttggg gtggaaagct gctcttcaat cctcgtcatg ctgaacttgt ttcagcatcc
     1201 atggcctgca ctccaattca gcgctgcgcc ggaggaaacg gcagaccata gaccctgaaa
     1261 caagttcagg gtgacgagaa atgagatgtc cacaaccggc cgaaaacgac catccaccag
     1321 catcagcctc attgtccccg ccccgccgcg cggctaaagc aacgcgcgtg accgcgcttt
     1381 ccctccgcat cgccgctttc gccgaccgcc ggccaaagct cctcgccctg ttgctcggcg
     1441 ccgtttcggc gacggggttc gcgccgttcc atctctggcc gctgacgctg ctggcgctcg
     1501 cgggctggat ggcgctcgtc gcgcgcagcc ccaaaggttc gcgcgcgctc ggcatcggct
     1561 gggcgttcgg ggtcggtcat ttcgtcgtcg gcctgaactg gatcgcgacc gcctttacct
     1621 atcaggcggc gatgcccgcg tggctgggct ggatcgcggt cgtgctgctg gcgctgtatc
     1681 tggcggtcta tccggcgctg accgcctggg gtgcgtggct ggtgaaaccc aagggcgata
     1741 cgcattttgc cgcaccgtcc tatctgctgg cgttcggcgg gctctggatt ctcaccgagt
     1801 ggctccgcag ctggattttt accggcttcg cgtggaatcc gatgggggtg atcgccgtcc
     1861 cgcacgctgc catgcccgcg cgctggatcg ggacctatgg catgagcggg atcgtcttcc
     1921 tgctcgcggg ggtcctgata ctggcgtggt ttcgccgccg cgtcgccgcc gccgcgctat
     1981 tcggcagcgt gctggttttc tggggcgccg cctttgtttc gcaaatgggc agcagcgccg
     2041 acgccgccgt tcgatccaag ggcatcgccc ggatacccgt caccgtcgtc cagcccaatg
     2101 tcggacagga ggataagtgg gagggcagca aggccgacgc caatttcgcc aagctcgcac
     2161 ggctgacgac gccgaaggat gacataccgc ggctgatcct gtggcccgaa gccgccgtgc
     2221 ccgactatct cgaacgcggc tatccgtcgg tctattacga ccgttcgccc gccgaagcgc
     2281 gcgcgcggat cgtccgcttg atgaacccga acgacgtcat gctgctcggt gcgctcaagc
     2341 tcgaactcga caaggcgggc gatgtcgtcg gcgcgcgcaa tgcggtgatg acggtgcatg
     2401 ccgacggcac gctcggaccg cgctacgaca aagcgcatct ggtgccctat ggcgaatatc
     2461 tgccgatgcg gccgatcctg tcggcgatcg gcctgtcgcg cctcgcgccc ggcgacatcg
     2521 atttctggcc cggacccggc ccccgcacgc tcgacctcgg cgccttcggc cgcgcggggc
     2581 tgcaaatctg ttacgagatc atcttttcgg gccaggtcgt cgatcgcgac catcgccccg
     2641 acttcatctt caacccgtcg aacgacgcct ggttcggcag ctggggcccg ccgcagcatc
     2701 tcgcgcaggc ccgcctccgc gcgatcgagg aaggcttgcc ggtgatccgt gcgactccca
     2761 ccgggatcag cgcggtgatc gacgccgacg ggcgcatcgt cgaatcgttg ccgatgcaca
     2821 aggcgggtcg catcgacacg gtggtgccgg ccgcgcgcgc accgacgctc tttgcgcgct
     2881 tcggcaacgc tctgcccgtc ggcttcgcgc tgctgttgct cgcgctggcc attgcgttcc
     2941 ggcgctcggg gcgctaacag cgcgcggaca taaagcatcc tttatattcg atttcgagaa
     3001 ggctttccca tgcgcaacag cttccttttc acctccgaga gcgtgtcgga gggtcatccc
     3061 gacaaggtcg ccgaccagat ttcggattcg atcgtcgacc tgttcctgtc gaaggatccc
     3121 gaggcgcgcg tggcgtgcga aacgctgacg acgacgcagc tcgtcgtgct cgcgggcgag
     3181 atccgctgca agggcgtgtt cgagaatgac gcctgggcgc cgggcgcgct cgatgagatc
     3241 gaggcgacgg ttcgcaacac ggtgcgcgaa atcggctatg agcagtcggg cttccactgg
     3301 cagagcttcc gcttcgagaa taatctccac ggccagtcgg cgcatatcgc gcagggcgtc
     3361 gacgagagcg gcgacaagga cgagggcgcc ggcgaccagg ggatcatgtt cggctatgcg
     3421 tcggacgaaa cccccgacct gatgcccgcg acgctcgatt acagccacaa gatcctcgag
     3481 cgcatggccg ccgaccgcaa ggcgggcacc gcgcccttcc tcgagcccga caccaagagc
     3541 caggtcacgc tgcgctatgc caatgagcgt ccggtcgaag cgaccgcgat cgtcgtgtcg
     3601 acgcagcatg cgccgggcta tttcttccac aacggcgaag gcgacgaagc gaaatatacc
     3661 gagctgcgca aatatgtgct cggcgtgatc gccgacgtgc tccccgccga gctgctcacc
     3721 gcgaacaccg tctatcacat caacccgacc ggccgtttcg agatcggcgg gcccgacggc
     3781 gacgccgggc tgaccgggcg caagatcatc gtcgacacct atggcggcgc gagcccgcac
     3841 ggcggcggcg cattcagcgg caaggacccg accaaggtcg accgttcggc ggcgtatatc
     3901 acgcgctatc tggcgaagaa tatcgtcgcc gcgggcctcg cgcgccgctg cacgatccag
     3961 ctgagctacg ccatcggcgt cgccgagccg ttgtcgatct atgtcgacct gcacggcacc
     4021 ggcacggtcg aggaaggccg catcgaggcc gcgatcccgc agctcgtgcg cctgacgccg
     4081 aagggtatcc gcacgcatct ggggctcaac aagccgatct accggcagac cgccgcctac
     4141 ggccatttcg gccgcaccgc cgagggcgac gccttcccgt gggagcgcac cgacctcgtc
     4201 gacaagctca aggccgcgct ggccgcctaa tgtgggccgg gggcgcttgc cgcccccgct
     4261 tcgccgcgct aacgggcgcg ccatgactgc gcataaaccc ggcgatccga cgaccctcaa
     4321 ccgcctttac ggccgatcga agggcaagcc gctgcgcgcg gggcagcagg atctggtcga
     4381 cacgctgttg ccgcagatcg cggtgcccga agttggcgaa gtttccgcgg agcgtctgtt
     4441 cgggcaggca tgtccgttgc attttgaaat cggttttggc ggcggcgagc atatggcggg
     4501 gcgcgccgac atgctgcccg accatggctt catcggcgcc gagccgttca tcaacggggt
     4561 ggcgcaggca ctcgtccatg tcgcgggcga tcatggcgag cggctgccgc tcggcaatgt
     4621 ccgcatccat catggcgacg cattggaagt gctgcggcgg attcccgacg gcgcgctttc
     4681 cttcgcctat ctgctccacc ccgacccctg gcccaaggcg cgccacgcga agcggcggat
     4741 gatgaacgac gggccgctcg acctgatcgc ggcgaagctg aaacccggcg gcgagttccg
     4801 tttcggcacc gaccatccga tctatctgca acacgcgctg atggtgatgc gccgccaccg
     4861 ccaccagtat gaatggctgg cgaaggacgc gcgcgatttc cagcagcgcc ccggcggctg
     4921 gcccgaaacg cgctatgaag ccaaggcgcg cgcgcagggg cacgaggtct ggtatttccg
     4981 ctacaagcgg atctagtcgc gcggctcgga cttttcggcc ttggcctgcc gcagccggtt
     5041 ttcgcgcatc gcgagttctt cgtaacggca cagcggcacc gcgggtttcg cggcgcgtgg
     5101 cgtttcgcgg cggggcttct ttcgggtcat gtcggcgcgc atcctaaccc gcgcgccgcg
     5161 cataaacaaa tggggcggcc tcgtatcgag tgccgcccca gttttgtgga tccgtcgggg
     5221 agagggagag gacggtccgg agattggtgt cttggtgtct tagcgcgagg ccatctggct
     5281 gtcggtttcg acttcggcaa cgccggcgat cttgagcgcg gcgcggaact gcttggcggc
     5341 ggcgcgttcg ttacccgctt cgcacagctt cttgccggtc gaaacgaagc gcttggcgct
     5401 cgactgcttg ctgccctcga cgccgctcgc ggcgacatgc gcctgttcgg cgagaccggc
     5461 gcagcgataa tcgccgcccg actgggcata agcgggggtc gtcgatgcca tcaggccggt
     5521 gaaggcgagc gccgaagcgg cgacgacggc aaaggcggtg ggaaagcggc ggaaagagga
     5581 gagctgatag gacatgggag aggttccttt cgtttcgttg tgcaaccttt ataggcgcgg
     5641 ttgcgcactt agataatcct ctataatctg caagtgcttt gaagcaggat ttaacaatcc
     5701 cccaaggggc gctcgacggg atcgaagcct tcctgcgtgt tgcggaaagg cgcagctttt
     5761 ccgccgcagc cgccgacctc ggcgtctccc cctccgcgat cagccagaca gtcaaggcgc
     5821 tcgaagcgcg cgtcggcgcg cccttgttca tgcgcacgac gcgcagcgtg ggcctcaccc
     5881 aggcgggcga gatgttcctc gagcgcgcgg cgcccgccta taccgggctc gccgacgcct
     5941 atgaagcggc gcgcaacctc ggcaaccggc ccgcgggacg gctgcggatc aatctgatgc
     6001 gcggcgcggt gcagccgctg ttcgaaccga tcatcgcggg cttttgcgaa acctatcccg
     6061 atatcgagct cgagatttac gccgacgatg ccctggcgga cctcagcgcc gggggcttcg
     6121 acgccggggt gcggatgggc gaatcgctcg acgccgacgt gatcgcggtg cgcctgaccg
     6181 ggccgttccg tttcgtcgtc gcggcggcgc ccgcctatct ggcgaagcac ggccgccccg
     6241 aaacccccga ggaactgcgc gaccatcgct gcatccgctt tcgcctcgcg accggcgcgc
     6301 tgatgccgtg gacgttcgag aagggaaacc gcgaatatga cgtcagcgtc accgggccgg
     6361 tgatcgtcaa cgactggatc gccgcgctcg tcgcgatccg cgccggcgtc gcgatgggca
     6421 tgatggccga accgatggcg aaggcgatgg tggaaaacgg cgatctcgaa ctggtgctca
     6481 ccgactatgc gtcggcgacc tcggggctct tcctctatta tcccggccgc aagcaggtga
     6541 tgcccaagct gcgcgcgttc atcgattatg tgcgcgaata tctgcccgac gagatcaccg
     6601 catgacgcgg tgaagcggtt cggcccggcg cggaaacaaa tccctttgcc gccagcagaa
     6661 aaattgggct ggcgcgcggc ggcgaatccc ggcagcaagt tgtcaactaa atgagttggg
     6721 atgaaatatg atcgatcttg cggccatcga cttcaccgcg ctcctgccgt tcatcctgat
     6781 cggcttcgcg gcgcagctcg tcgacggcgc gctcggcatg gcgttcgggg tgatctgtaa
     6841 cacgttgctc gtcgcggtgc tgggcgtccc gcccgcgacc gcgtcggcgc gcatccatgt
     6901 cgtcgagatt ttcaccaccg gcgtgtcggg gctgagccat gtgttccacc gcaatatcga
     6961 ctggccgctc ttctttcgcc tgctgatccc cggggtgatc ggcggcgtgc tcggcgccta
     7021 tgtgctgtcg tcgctgcacg ccgaggtcgt gaagcccttc gtgctcggct atctcgtgct
     7081 gatcggcatc tggttgctga tccgcggcgt gctgtatccg cccaaattct cgaagcccaa
     7141 ggtcgtcgcg ccgctcgcgg tggtcggcgg cttcctcgac gccgcgggcg gcggcggctg
     7201 ggggccggtc gtcacctcga acctgctcgt ccagggctgc gagccgcgta aggtcgtcgg
     7261 cacggtcaac agcgtcgaat tcttcctcgc ggtcgcggtt tcggccgcct tcatctggca
     7321 tctcggcatc ggcgaaatcc tgggtccgac gctggggctg atcatcggcg gggtcgtcgc
     7381 cgcgccgatc ggcgcgatga tggcgaaacg tttctcgccc aaggtcatgc tgatgctggt
     7441 cggcaccgtg ctgaccgcga cgagcgcgtt cggtctgtac aaggtgctgg tgggctagcg
     7501 gggtgatggc gaccggaagc tgccatatcc tctcccctcc cttctaggga ggggccgggg
     7561 gtgggtagc
//
LOCUS       DATMSU010000098         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_68272, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000098 DATMSU010000000
VERSION     DATMSU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68272"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VN034_02600"
     CDS             complement(<1..304)
                     /locus_tag="VN034_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HWV59516.1"
                     /translation="MKSDSRKTFHVKSFGCQMNVYDGERMAEMLGAEGYVAAADGADA
                     DLVVLNTCHIREKAAEKVYSDIGRLKRADGSTPTIAVAGCVAQAEGAEIARRAPSVD"
     gene            complement(312..1097)
                     /locus_tag="VN034_02605"
     CDS             complement(312..1097)
                     /locus_tag="VN034_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWV59517.1"
                     /translation="MSPSIASATDRSAITWRTVARLALMVLALLFLIVPHLLYRAVGR
                     PSPMVMAFLNAAGWIAGLRIRTTGTRLRRDVLFVSNHVSWLDILALGGAARSAFVSKA
                     EIGSAPLVGWLADQNHTIYVQREARREIHNQANDLRNALARGRPVTLFPEGTTGPGDG
                     LLPFRASLFQAVIPTPPKLRVQPVLLDYGAEANDIAWLNPESGLGNFKRLLARKRPVH
                     LTIHYLDPLPDIAEGDRKLLAEAARAAIAGEIARSSASAPHRL"
     gene            complement(1069..1494)
                     /locus_tag="VN034_02610"
     CDS             complement(1069..1494)
                     /locus_tag="VN034_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWV59518.1"
                     /translation="MSGNIDLEALCHEKGLRITEQRRIIARVISDSEDHPDVETLYER
                     ASKVDSGISIATVYRTVRLFEEAGILDRHDFGDGRSRYEAAPEAHHDHLIDVETGKVI
                     EFVDPELEALQKVIAERLGFRLVDHRMELYGVAIDRKRD"
     gene            complement(1630..2064)
                     /locus_tag="VN034_02615"
     CDS             complement(1630..2064)
                     /locus_tag="VN034_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWV59519.1"
                     /translation="MSDQADTNEMLVTLTADIVAAHVSNNSVAISDLSILINNVHAAL
                     SGLGNEPVVEEKLVPAVSIRTSVKPDYIVCLEDGKKLKMLRRHLMTHYGMTPDDYRAK
                     WGLAADYPMVAPNYAEKRRALAKEIGLGTKGRGGGRKRKAKA"
     gene            complement(2173..2655)
                     /locus_tag="VN034_02620"
     CDS             complement(2173..2655)
                     /locus_tag="VN034_02620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV59520.1"
                     /translation="MTAAIRLATARVGDLAAVMRVMDAAFEPAYGEAWTGAQLLTLFA
                     LPSARVCLAWDGEEACGFSAARIAGPESELLLLAVDPQFRGRGIGRLLIDDWRAWATG
                     EGAEDYFLEMRADNDAVHLYERAGFTECGRRPAYYRGNDGKLRDAITMRRPARESRAD
                     "
BASE COUNT          475 a          869 c          898 g          501 t
ORIGIN      
        1 catcgacgct gggcgcacgg cgcgcgatct cggcgccctc ggcctgcgcg acgcagccgg
       61 cgacggcgat ggtcggcgtg ctgccgtcgg cgcgcttcag ccggccgatg tccgaataga
      121 ctttctcggc cgccttttcg cggatgtggc aggtgttgag aacgaccaga tcggcgtcgg
      181 cgccatcggc ggccgcgaca tagccctctg ccccgagcat ctcggccatg cgttcgccgt
      241 catagacgtt catctggcag ccgaaggatt tgacgtggaa agtcttgcga gagtcggatt
      301 tcatcggcgc gctataggcg atgcggcgcg ctggcggaag accgcgcaat ttcccccgcg
      361 atcgccgcgc gcgcggcctc ggcgagcagc ttgcgatcgc cctcggcaat atcggggagc
      421 ggatcgagat aatggatcgt caaatgaacc gggcgcttgc gtgcgagcaa ccgcttgaaa
      481 ttgccgaggc ccgattcggg attgagccac gcgatgtcgt tcgcctcggc gccatagtcg
      541 agcaggaccg gctggacgcg cagcttcggc ggcgtcggga tcaccgcctg gaacagcgag
      601 gcccggaagg gcagcaggcc gtcgcccggg cccgtcgtgc cttcggggaa caaggtcacc
      661 gggcgcccgc gcgcaagcgc attcctgagg tcgttcgcct gattgtggat ctcgcgccgc
      721 gcctcgcgct ggacatagat ggtgtggttc tggtcggcga gccagccgac gagcggggcg
      781 gagccgatct ccgccttcga tacgaacgcc gagcgcgccg cgccgcccag cgcgagaatg
      841 tcgagccagc tgacatggtt cgagacgaac agcacgtcgc gccggagccg cgtgcccgtc
      901 gtgcggatgc gcagccccgc gatccagccg gcggcgttga ggaaagccat caccatcggc
      961 gagggacggc cgacggcacg gtagagcaga tgcgggacga tcaggaaaag gagcgccagc
     1021 accatcaggg cgagacgggc gaccgtccgc caggtaatcg cgctgcgatc agtcgcgctt
     1081 gcgatcgatg gcgacaccgt aaagctccat gcggtggtcg acgaggcgga agcccagccg
     1141 ttcggcgatc accttctgca acgcctcgag ctccggatcg acgaattcga tcaccttgcc
     1201 cgtttcgacg tcgatcagat ggtcgtgatg cgcctcgggc gccgcttcgt agcgcgagcg
     1261 cccgtcgccg aaatcgtggc ggtcgaggat gcccgcctct tcgaacaggc gcacggtgcg
     1321 gtaaacggtc gcgatcgaga tgccgctgtc gaccttcgac gcgcgttcat agagggtctc
     1381 gacgtcggga tgatcctccg aatccgaaat gacgcgcgcg atgatgcggc gctgttcggt
     1441 gatgcggagg cccttttcgt ggcacagggc ttcgagatcg atattgccgg acatgcttgc
     1501 ctttcctgca gatgcagccg tgtctgtctt tgttacaaac tgtataggga cgccccgcga
     1561 ataggacaag ggcggcgccc ttgcgggtac cgcccttccc tgccccgcgg gggcgtcatt
     1621 tgcgagccgt cacgccttgg ctttgcgctt gcgtccgcca ccacgcccct tggtgccgag
     1681 gccgatttct ttcgccagcg cgcggcgctt ttcggcatag ttgggcgcga ccatcggata
     1741 gtcggccgca aggccccatt tggcgcgata gtcgtcgggg gtcatgccat aatgcgtcat
     1801 caggtggcgg cgcagcatct tcagcttttt gccgtcctcg agacagacga tatagtcggg
     1861 cttgaccgag gtgcggatcg aaaccgccgg caccagcttt tcttccacga ccggctcgtt
     1921 cccgagaccc gacagcgcgg cgtggacatt gttgatcaga atggagaggt ctgaaatggc
     1981 gacgctgttg ttgctgacat gcgccgcgac aatgtcggcg gtcaacgtaa cgagcatctc
     2041 attggtgtcg gcttgatcgg acatgagact attccttgtt gtcgattacc cataagcatg
     2101 tgagcgaata ttggattttt tctttcgccg gatggattag tgccactttg gatgtaattc
     2161 cgcaatgacg gatcagtcgg ctcgactctc cctggccgga cgccgcatcg taatggcatc
     2221 gcggagtttg ccgtcgttgc cgcgataata ggccgggcgc cgcccgcact ctgtaaagcc
     2281 cgcgcgttca taaagatgaa ccgcgtcatt gtctgcccgc atttcaagga aatagtcttc
     2341 ggcaccctct cctgtagccc aggcgcgcca gtcgtcgatc aataatcgcc cgatcccgcg
     2401 gccgcgaaac tgcggatcga ccgcgagcaa caataattcg ctttcggggc ccgcgatccg
     2461 cgcggcggaa aagccgcacg cctcttcacc gtcccacgcc agacatacgc gcgccgacgg
     2521 caacgcgaac agggtcaaga gctgcgcgcc ggtccatgct tcgccatagg cgggctcgaa
     2581 cgccgcatcc atcacgcgca tcaccgcggc cagatcgccg acgcgcgcgg tggcgaggcg
     2641 aatagcggcg gtcatgcacg cgccgccatc ggcttcgcgt cgggcgcgcg gccatagatc
     2701 gggctcggcc ggtcgatcat cgcggtttcg ggcagtcgca aaa
//
LOCUS       DATMSU010000099         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_125357,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000099 DATMSU010000000
VERSION     DATMSU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125357"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VN034_02625"
     CDS             complement(<1..254)
                     /locus_tag="VN034_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWV59521.1"
                     /translation="MSHDTHAHDAHHEIEMPHASMRDYVIGFLLSVILTAIPFWLVMT
                     MPLSAGAIGAIIMGFAVVQIVVHMVFFLHMTPKAEGGWSLT"
     gene            complement(251..892)
                     /gene="cyoC"
                     /locus_tag="VN034_02630"
     CDS             complement(251..892)
                     /gene="cyoC"
                     /locus_tag="VN034_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HWV59522.1"
                     /translation="MSAKTMTANAVAADEPVRFYDLDEHAHPEGHSTMLGFWIYLMSD
                     CLIFAILFACYAVLGGSYAAGPAPKDLFDLKLIALNTAMLLFSSITYGFAVLQMQQGR
                     VKGVQLWLAITGLFGLAFLGIELYEFHHLIEIGAGPQRSAFLSSFFTLVGTHGLHVTF
                     GIVWLVTLMVQLSKHGLIAANKRRVMCLSMFWHFLDVVWIGVFTFVYLMGVLR"
     gene            complement(897..2906)
                     /gene="cyoB"
                     /locus_tag="VN034_02635"
     CDS             complement(897..2906)
                     /gene="cyoB"
                     /locus_tag="VN034_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HWV59523.1"
                     /translation="MIPHPAPETGPILGKLSLEALPLHEPILVITFIGVVIGGIAVLG
                     LITKFRLWGYLWKEWFTTVDHKRIGIMYMILGLIMFLRGFADAIMMRLQQAMAFGGSE
                     GYLTPHHYDQVFTAHGVIMIFFVAMPFITGIMNYIVPLQIGARDVSFPFLNNFSFWMT
                     TAGAVLTMISLFVGEYAQTGWLAYPPLSGIAYSPNVGVDYYIWGLQIAGVGTTLSGIN
                     LVVTILKLRAPGMGLMKMPVFTWTSLCSNILIVASFPILTATLVLLTLDRYVGTNFFT
                     NDLGGSPMMYVNLIWIWGHPEVYILVLPLFGVFSEVTSTFSGKRLFGYTSMVYATVCI
                     TILSYIVWLHHFFTMGSGASVNSFFGITTMVISIPTGAKLFNWLFTMYRGRIRFELPM
                     MWTVAFMLTFVIGGMTGVLLAVPPADFVLHNSLFLIAHFHNVIIGGVLFGLFAAINYW
                     WPKAFGYKLDVFWGKVSFWCWVVGFWVAFTPLYILGLMGVTRRMRVFDDPSLQIWFVI
                     AGIGALIVAAGIGAMLVQFAVSIWRRKELRDETGGDPWDGRTLEWATSSPPPDYNFAF
                     TPVIHDLDAWYDMKKRGAQRPVDGYRDIHMPSNTGAGIILSGICLVMGFALVWHIWWL
                     VAASFVAVIAAAIIHTFNYKRDFDIPAATVTAVEEARTRQLAAGA"
     gene            complement(2924..4105)
                     /gene="cyoA"
                     /locus_tag="VN034_02640"
     CDS             complement(2924..4105)
                     /gene="cyoA"
                     /locus_tag="VN034_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HWV59524.1"
                     /translation="MRSNLPRFPARLRLLPLLAALPLLSGCGLIVLDPAGDVARQQGD
                     LIVLSTVLMLLIIVPVMALTIFFAWKYRASNKDATYKPDWDHSTQLELVIWSAPLLII
                     ICLGAVTWVATHLLDPYRPLARTAPGQPVAAEVQPLDVQVVALDWKWLFIYPEQGVAT
                     VNELALPVNRPVRFRISSSSVMNSFYVPALAGQIYAMPGMETKLHGVFDKTGEFEGFS
                     ANYSGWGFSNMRFAVKSLPAGEFDQWVAATKASAEGDLSRAEYLKLEKPTHKEPVRRF
                     AASDPQLFDAVVNMCVEPGKMCMHDMMSVDAAGGAGIAGIGNVRQVTYDKFAARGTAD
                     LARWSMRYVAAACSSPVMSNDRPDAKPVAPEMLKGEGLPVPAKPERTALRASSTARPM
                     S"
     gene            4275..5609
                     /locus_tag="VN034_02645"
     CDS             4275..5609
                     /locus_tag="VN034_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59525.1"
                     /translation="MAADTVPTTEAERDARALHGAEDGSHRIDPAEIAIGVIIGRTSE
                     FFDFFVYAIASVLVFPKLVFPHLDPLTGTLWSFAIFALAFVARPVGTIIFTAIDRAYG
                     RGAKLTIALFLLGGSTAAIAFLPSYESVGVGAALFLALFRMGQGVALGGSWDGLASLL
                     AINAPENRRGWYAMIPQLGAPLGLIVASLLFMFLIAALPAEDFLAWGWRYPFFVAFAI
                     NVVALFARLRIVVTPEYAELFENRALEPAPLLETVRSEWKTIVIGTFAPLASFAMFHM
                     VTVYPLSWVFLFTDESPVRFLVIEAIAAVVGVIAIVASGILADRVGRRTLLAVTAAAI
                     AAFSGFAPQLLDAGQFGEATFMILGFALLGLSFGQSSGALSSNFQPRHRYTGSAFTSD
                     LAWLFGAGFAPMVALWLSSQFGLIAAGAYLLSGALGTLVALWLNRELASTID"
     gene            5671..>5862
                     /locus_tag="VN034_02650"
     CDS             5671..>5862
                     /locus_tag="VN034_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV59526.1"
                     /translation="MRHLARFDLNLLRIFDAIFAKGGVSAAARHLNLSQPAISHALAR
                     LRTQFDDPLFVRQGNRLVPT"
BASE COUNT         1087 a         1927 c         1850 g          998 t
ORIGIN      
        1 gtcagcgacc agccgccctc ggcctttggc gtcatatgga ggaaaaagac catatggacg
       61 acgatctgga ccaccgcgaa acccatgatg atcgcgccga tcgccccggc gctcagcggc
      121 atcgtcatca ccagccaaaa gggaatggcg gtcaggatca ccgacagcag gaagccgatg
      181 acatagtcgc gcatcgacgc gtgcggcatc tcgatctcgt ggtgcgcgtc gtgcgcgtgc
      241 gtatcgtggc tcaccgcagc actcccatca gatagacaaa ggtgaagacg ccgatccaca
      301 cgacgtcgag gaagtgccag aacatcgaaa ggcacatcac gcggcgcttg ttcgcggcga
      361 tcagcccgtg cttcgacagc tggaccatca gcgtcaccag ccagacgatg ccgaaggtga
      421 cgtgcagccc gtgcgtgccg acgagcgtga agaagctcga caggaaagcg ctgcgctgcg
      481 gccccgcgcc gatctcgatc agatggtgga attcgtaaag ctcgatcccg aggaaggcga
      541 gcccgaacag gccggtgatc gcgagccaga gctggacgcc cttcaccctg ccctgctgca
      601 tctggagtac cgcgaaacca taagtgatcg acgagaagag cagcatcgcg gtgttgagcg
      661 cgatcagctt gaggtcgaac agatcctttg gcgcggggcc tgcggcatag ctgccgccga
      721 gcacggcata gcaggcaaag aggatcgcga agatgaggca gtcgctcatc agatagatcc
      781 agaagcccag catcgtgctg tgcccctcgg gatgcgcatg ctcgtcgagg tcgtagaagc
      841 ggacgggctc gtcggcggcg accgcgttag ctgtcatggt cttggcgctc atcgcattag
      901 gctccggccg ccagctggcg ggtgcgcgct tcctcgaccg cggtgaccgt cgccgccgga
      961 atgtcgaaat cgcgcttata gttgaaggtg tggatgatcg cggcggcgat caccgcgacg
     1021 aagctcgccg cgacgagcca ccagatgtgc cagacgagcg cgaaacccat gacgaggcag
     1081 atgcccgaca ggatgatccc cgcgccggtg ttcgacggca tgtggatgtc gcgatagcca
     1141 tcgaccgggc gctgagcccc gcgcttcttc atgtcgtacc aggcgtcgag gtcgtggatc
     1201 accggcgtga acgcgaaatt atagtcgggc ggcggcgagc tcgtcgccca ttcgagcgtg
     1261 cggccgtccc acggatcgcc gccggtttcg tcgcgcaatt ccttgcggcg ccagatgctc
     1321 accgcgaact ggaccagcat cgcaccgatg cccgcggcga cgatcagcgc gccgatcccc
     1381 gcgatcacga accagatttg caggctcggg tcgtcgaaca cgcgcatccg ccgcgtcacg
     1441 cccatcaggc cgaggatata gagcggggtg aaggcgaccc agaagcccac gacccagcac
     1501 cagaagctga ccttgcccca gaagacgtcg agcttgtagc cgaacgcctt gggccaccag
     1561 taattgatcg ccgcgaacag accgaacagc acgccgccga tgatcacatt atggaaatgc
     1621 gcgatcagga acagcgagtt gtggagcacg aagtcggcgg gcggcaccgc gagcagcacc
     1681 ccggtcatgc cgccgatgac gaaggtcagc atgaaggcga ccgtccacat catcggcagt
     1741 tcgaaccgga tgcggccgcg atacatggtg aacagccagt tgaagagctt ggcccccgtc
     1801 gggatcgaga tcaccatcgt cgtgatgccg aagaagctgt tgacgctcgc ccccgacccc
     1861 atggtgaaga aatggtgcag ccagacgatg tacgacagga tcgtgatgca caccgtcgca
     1921 tagaccatcg aggtatagcc gaagaggcgc ttgcccgaga aggtcgaggt gacttcggaa
     1981 aagacgccga acagcggcag cacgaggata tagacctcgg gatgccccca gatccagatc
     2041 aggttcacat acatcatcgg cgatccgccg agatcgttgg tgaagaaatt ggtgccgaca
     2101 taacggtcga gcgtcagcag cacgagcgtc gcggtcagga tcgggaagga ggcgacgatc
     2161 aggatgttcg agcagagcga ggtccaggtg aagacgggca tcttcatcag ccccatcccc
     2221 ggcgcgcgca gcttcaggat cgtgacgacc aggttgatgc ccgacagggt ggtgccgacg
     2281 ccggctatct gtaagcccca gatataatag tcgacgccga cattggggct gtaggcgatc
     2341 cccgacagcg gcggataggc gagccagccg gtctgggcat attcgccgac gaacagcgag
     2401 atcatcgtca gcaccgcgcc cgcggtcgtc atccagaaac tgaaattgtt aaggaaggga
     2461 aaactgacgt cgcgcgcacc gatctgcagc gggacgatat agttcatgat ccccgtgatg
     2521 aacggcatcg cgacgaagaa gatcatgatc accccgtgcg cggtgaacac ctggtcataa
     2581 tggtgcgggg tcagatagcc ctcggacccg ccgaacgcca tcgcctgctg caaccgcatc
     2641 atgatcgcgt cggcaaaacc gcgcaggaac atgatcaacc ccaggatcat gtacatgatc
     2701 ccgatgcgct tgtggtcgac ggtggtgaac cattccttcc acaaatagcc ccagaggcgg
     2761 aatttggtga tcaggccgag caccgcgatg ccgccgatga cgacgccgat gaaggtgatg
     2821 acgaggatcg gctcgtgcag cggcagcgcc tcaagcgaca gcttgccgag gatcgggccg
     2881 gtttcgggtg cgggatgcgg gatcataagg gtctcggctc ggttcaggac atcgggcggg
     2941 cggttgagga ggcgcgcagc gccgtgcgtt cgggcttggc cgggaccgga agcccctcgc
     3001 ccttcagcat ttccggcgcc acgggcttcg cgtcggggcg gtcgttcgac atcaccggcg
     3061 aactgcaagc ggccgcgaca tagcgcatgc tccagcgcgc gagatcggcc gtcccgcgcg
     3121 cggcgaactt gtcataggtc acttgccgga cattgccgat ccccgcgatc cccgcgccgc
     3181 ccgcggcatc caccgacatc atgtcgtgca tgcacatctt gccgggctcg acgcacatgt
     3241 tgaccaccgc gtcgaacagt tgcggatcgc tcgcggcaaa gcggcggacg ggttccttgt
     3301 gcgtcggctt ttccagcttg agatattcgg cgcggctgag gtcgccttcg gccgaagcct
     3361 tcgtcgccgc gacccattgg tcgaactcgc cggcgggcag gctcttcacc gcgaagcgca
     3421 tgttcgaaaa gccccagccc gaataattgg ccgaaaagcc ctcaaattcg ccggtcttgt
     3481 cgaacacgcc gtgcagcttg gtttccatgc ccggcatcgc gtaaatctgc cccgcgagcg
     3541 cgggcacata aaagctgttc atcaccgagg aagaggagat gcggaaccgc accggccggt
     3601 tcaccggcag cgccagctcg ttcaccgtcg cgacgccctg ttcggggtag atgaacagcc
     3661 atttccagtc gagcgcgacg acctggacgt cgagcggctg gacctccgcc gcgaccggct
     3721 ggcccggcgc cgtgcgcgcg agcgggcgat aggggtcgag caaatgcgtc gcgacccatg
     3781 tcaccgcgcc gaggcagatg atgatcagca gcggcgccga ccagatgacg agttcgagct
     3841 gcgtcgaatg gtcccagtcg ggcttgtagg tcgcgtcctt gttcgacgcg cgatatttcc
     3901 acgcgaagaa gatcgtcagc gccatcaccg gcacgatgat cagcagcatc agcaccgtcg
     3961 acaggacgat caaatcgccc tgctggcgtg cgacgtcgcc ggcgggatcg agcacgatca
     4021 gcccgcagcc cgacaaaagg ggcagcgcgg cgagcagcgg caacagccgc aatcgggcgg
     4081 gaaaacgggg gagattgctt cgcatagagc gcgcctaagc ctcctttgtt gcactgcaca
     4141 taggacattt tgtccaatcc cgcgatcgtc catcttcgcg caggggcatt ttcattcggg
     4201 gcgattctgc cctatgaccg actcgtgccg ccgcatcctg cgcaagcgga ggcgccagct
     4261 caaggattgt taccatggct gccgacaccg tccccaccac cgaggccgaa cgcgatgccc
     4321 gcgccctcca cggcgccgag gacggcagcc accgcatcga ccccgccgag atcgcgatcg
     4381 gcgtcatcat cggacggacg tcggaattct tcgacttttt cgtctatgcg atcgcgtcgg
     4441 tgctggtgtt cccgaagctc gtcttcccgc atctcgaccc gctgacggga acgctctggt
     4501 ccttcgcgat cttcgccctc gccttcgtcg cgcgcccggt cggcacgatc atcttcaccg
     4561 cgatcgaccg cgcctatggc cgcggtgcca agctcacgat cgcgctcttc ctgctcggcg
     4621 gttcgaccgc ggcgatcgcc ttcctgccga gctacgagtc ggtcggcgtc ggcgccgcgc
     4681 tgttcctcgc gctgttccgc atgggccagg gtgtcgcgct cggcggatcg tgggacggcc
     4741 tcgcctcgct gctcgcgatc aacgcccccg aaaaccgccg cggctggtac gctatgatcc
     4801 cgcagctcgg cgcaccgctc ggcctcatcg tcgccagcct gctcttcatg ttcctgatcg
     4861 ccgcgctgcc ggccgaggat ttcctcgcat ggggctggcg ctatcccttc ttcgtcgcct
     4921 tcgcgatcaa tgtcgtcgcg ctgttcgcgc ggctgcgcat cgtcgtcacc cccgaatatg
     4981 ccgagctgtt cgagaaccgc gcgctcgaac ccgcgcccct gctcgaaacg gtgcgctcgg
     5041 aatggaagac gatcgtcatc ggcaccttcg cgccgctcgc cagcttcgcg atgttccaca
     5101 tggtcaccgt ctatccgctg tcgtgggtgt tcctgttcac cgacgaaagc ccggtgcgct
     5161 tcctggtgat cgaagcgatc gccgcggtcg tcggcgtcat cgcgatcgtc gcgtcgggca
     5221 tcctcgccga ccgcgtcggc cgccgcacgc ttcttgccgt caccgccgcc gccatcgcgg
     5281 cgttcagcgg cttcgcgccg caactgctcg acgcgggcca attcggcgaa gcgacgttca
     5341 tgatcctcgg cttcgcgctg ctcggcctgt cgttcggtca gtcgtcgggc gcgctctcgt
     5401 cgaacttcca gccgcgccac cgctataccg gatcggcctt cacctccgac ctcgcctggc
     5461 tgttcggcgc cggcttcgcg ccgatggtcg cgctgtggct gtcgagccag ttcgggctga
     5521 tcgcggcggg cgcctatctg ctgtcgggcg cgctcggcac gctcgtcgcg ctgtggctca
     5581 accgcgaact ggcgagcacg attgactgat cgacgcgatc gaaccgggat cggctatcga
     5641 tcccgcgccg gcttcgctta ggagccggcc ttgcggcatc tcgcccggtt cgatctcaac
     5701 ctgctgcgca ttttcgacgc gatcttcgcc aagggcggcg tgtcggcggc ggcgcggcac
     5761 ctcaacctgt cgcagccggc gatcagccac gcgctcgcac ggctgcgcac ccagttcgac
     5821 gatccgctct tcgtgcggca gggcaatcgc ctcgtcccga cc
//
LOCUS       DATMSU010000100         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_162798,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000100 DATMSU010000000
VERSION     DATMSU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162798"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VN034_02655"
     CDS             complement(<1..348)
                     /locus_tag="VN034_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59527.1"
                     /translation="MLTAAMLLGLAWKSLVVAALTLALLRLARARSAGERSMIAHAGL
                     AALLALPAAILLLPQWAPLPASWFAAPALETGATGGSAIDPAAATASVPVMVDRAGST
                     PIVVDWSDLAPFLY"
     gene            complement(348..722)
                     /locus_tag="VN034_02660"
     CDS             complement(348..722)
                     /locus_tag="VN034_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWV59528.1"
                     /translation="MLSSLPPREREIVDILYERGASTVTEIGDALADPLSGSAIRAML
                     KRLETKGFVVREGSERGFVYAPSVSDKTARKSALSQVVRVFFNGSATSAAAALLGMQD
                     EMTGDELDELEKMIAKAREGRG"
     gene            complement(1080..1808)
                     /locus_tag="VN034_02665"
     CDS             complement(1080..1808)
                     /locus_tag="VN034_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWV59529.1"
                     /translation="MANWNDPNMAASGFGAGANAMDQAVDAGLRSYMLSVYNYMGSGV
                     LLTGIVAMLAFNSGFTASMIGSPLMWVVMLAPLAFVLVLSFGINKLSTGAAQALFWVY
                     AAVMGLSMSTIFLAFTATSIATTFFATAAAFLGLSLYGYTTKKDLSGFGTFLIMGVVG
                     ILVAMVINMFVQSSALGLAISVIGVLLFAGLTAYDTQKIKSMYFYVRGTDFVGKSVIM
                     GALTLYLDFVNMFTFLLNLMGSRE"
     gene            1974..2591
                     /gene="thpR"
                     /locus_tag="VN034_02670"
     CDS             1974..2591
                     /gene="thpR"
                     /locus_tag="VN034_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWV59530.1"
                     /translation="MSTHRLFVALRPPRPVRDILLAAMHGISGARWQSDEQLHLTLRF
                     IGEVDRHRAEDIAAALGAIYAPAVTARIAGVDLFERQGRPHMLWAGVEPHAPLGALHR
                     KVDQLLARVGVAPETRAFVPHVTLARLNRASGPVAPFLALNSDLASPPFEFGHVTLYE
                     SEMGHGGSRYHPVARYPLGENSATSAEAIASTSSHVEAKPSSFPK"
     gene            complement(2498..>2844)
                     /locus_tag="VN034_02675"
     CDS             complement(2498..>2844)
                     /locus_tag="VN034_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HWV59531.1"
                     /translation="GDWHVGHAPDGRAQAEARRRGIDISTLRARQLSRDDFYDFDLIL
                     AADETNLRDARAIRPADATADLRLMLDLLPGRKGQGVTDPYFGKDDGFASTWDDVDAI
                     ASALVAEFSPKG"
BASE COUNT          522 a          926 c          920 g          476 t
ORIGIN      
        1 atagagaaag ggggcaaggt cgctccagtc gacgacgatc ggcgtcgatc cggcccgatc
       61 caccatgaca gggactgaag ccgtggcggc ggccggatcg atcgccgatc ccccggttgc
      121 tcctgtttcg agagcgggcg cggcaaacca gctcgcaggc agcggcgccc attgcggcag
      181 cagcaggatc gcggcgggca gcgcaagcag cgccgcgagc cccgcatgcg cgatcatcga
      241 ccgttcaccc gccgaacgcg cccgcgcaag acggagcagc gccagcgtca gcgccgcgac
      301 gacgagtgat ttccaggcga ggcccagcaa catggcggca gtcagcatca ccctctcccc
      361 tcgcgtgcct tggcgatcat cttttccagc tcgtcgagct cgtcgcccgt catctcgtcc
      421 tgcatgccga gcagcgccgc ggcggcgctc gtcgccgatc cgttgaagaa gacgcgcacg
      481 acctggctca gcgccgactt gcgcgcggtc ttgtccgaca cgctcggcgc atagacgaag
      541 ccgcgttcgg acccctcgcg cacgacgaag cccttggtct cgagccgctt gagcatcgca
      601 cggatcgccg agcccgacag cgggtcggcc agcgcgtcgc cgatctcggt gaccgtggag
      661 gcgccgcgct cgtacaggat gtcgacgatt tcgcgttcgc gtgggggcag actggacagc
      721 attcgaatca cctcatgcgc tacatttgta gcgtgctaca tttgtagcgc atgcttggca
      781 aggggtttta ttcgaataag acacggcgtt cgtcgagcca cggttcgggg tggatgagca
      841 ggcccaagcg aatgcacccg ccggcagggg ccgcttttgg tgcgctcgat tcctcaagtc
      901 tcgtcaccct gaacttgttt cagggtccat ggcctgccct cgaaagcgac gcggcgtgga
      961 agaagagagc aggccatgga tgctgaaacg agttcagcat gatggagaaa agccagccaa
     1021 agaaagagcc cggcggatcg ctccgccggg cccctctatg tcgtccgtgt ccggcccgct
     1081 tattcgcggc tgcccatcag gttgagcagg aaggtgaaca tgttgacgaa gtcgagatag
     1141 agcgtcagcg cgcccatgat caccgacttg ccgacaaagt cggtcccgcg gacatagaaa
     1201 tacatgctct tgatcttctg cgtatcatag gcggtgaggc ccgcgaagag cagcacgccg
     1261 atgacgctga tcgcgaggcc gagcgcgctc gactgcacga acatgttgat gaccatcgcg
     1321 acgaggatgc cgacgacgcc catgatcagg aaggtgccga agcccgacag atccttcttg
     1381 gtcgtatagc catagaggct aagaccgagg aaggccgccg cggtcgcgaa gaaggtcgtc
     1441 gcgatcgacg ttgcggtgaa ggcaaggaag atcgtcgaca tcgacaggcc catcaccgcg
     1501 gcatagaccc agaacagcgc ctgtgcggcg ccggtcgaga gcttgttgat gccgaagctc
     1561 agcaccagca cgaaggccag cggcgcgagc atcaccaccc acatcagcgg gctgccgatc
     1621 attgacgcgg taaagccgct gttgaacgcc agcatcgcga cgatgccggt cagcagcacg
     1681 cccgagccca tatagttgta aaccgacagc atgtacgacc gcaggccggc atcgacggcc
     1741 tggtccattg cgttcgcgcc ggccccgaaa ccggaagccg ccatattggg gtcgttccaa
     1801 ttcgccattt tgcatctcca tcaccggcca gcccataccg gccgtatgca gcaatatcgg
     1861 gggtttcccc tcccgtttca agcgaaaccg gacatcagca atattccagc aatattattg
     1921 tctcgcgcag tttcgggtcg cggtccgaaa catcgccgct tatgctgccc ggcatgtcca
     1981 cgcaccgtct gttcgtcgcg ctgcgcccgc cgcgtccggt ccgcgacatc ttgctcgcgg
     2041 cgatgcacgg tatttcgggc gcgcgctggc aaagcgacga gcagcttcac ttgaccttgc
     2101 gcttcatcgg cgaggtcgac cgccaccgcg ccgaagatat cgcggcggcg ctcggcgcga
     2161 tttacgcgcc cgcggtcacg gcgcggatcg ccggtgtcga cctgttcgaa cgacaggggc
     2221 ggccgcatat gctgtgggcg ggggtcgagc cgcacgcgcc gctcggcgcg ctgcaccgca
     2281 aggtcgacca attgctggcg cgcgtcgggg tcgcacccga aacgcgcgcc ttcgtgccgc
     2341 atgtcacgct ggcgcggctg aaccgcgcgt cggggccggt cgcgcccttc cttgcgctga
     2401 acagcgatct tgccagcccg ccgttcgaat tcggccatgt cacgctctat gaaagcgaga
     2461 tgggccatgg cgggtcgcgc tatcatccgg tggcgcgtta ccccttgggc gaaaattcgg
     2521 cgacgagtgc tgaggcgatc gcatcgacgt cgtcccacgt cgaagcgaaa ccgtcatcct
     2581 tcccgaaata ggggtcggtc acgccctgcc ctttgcggcc gggaaggagg tcgagcatca
     2641 gccggaggtc ggcggtcgca tcggccgggc ggatcgcccg cgcatcgcgc aggttcgtct
     2701 cgtcggcggc gaggatcagg tcgaaatcat agaagtcatc gcgcgacaat tggcgcgcgc
     2761 gcagcgtcga tatgtcgatg ccgcggcggc gcgcttcggc ctgagcgcgc ccgtcggggg
     2821 cgtggccgac gtgccagtcg ccgg
//
LOCUS       DATMSU010000101         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_87331, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000101 DATMSU010000000
VERSION     DATMSU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87331"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VN034_02680"
     CDS             <1..1247
                     /locus_tag="VN034_02680"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV59532.1"
                     /translation="KRRLDAAPGAAQSDAMLSLLALLTSVASPAPAAVAVHFNRGGQV
                     EARVVEGLADRAGGRAITPDDPVRVASVSKLVVALGVLRLVEAGKLDLDRDVSDYLGW
                     RLRHPAYPDRSISLSQLLRHRSGLNDETNYALPLDADLEAALRDPAAWDKARPPGGDF
                     AYANFNYPVVAAAIEGATGERFDQAMTRLVFRPLGLDACFNWPTCSDAAVARAVVLHD
                     AEGNVVRDDMKGARPACPVVPATNGSCDLGTYRLARHGAGFSPQGGLRISANGLARIG
                     VMLLRRGEGFLKAESLARLEAMAPVRPTSGEGTGGFFCEYGMAMHSTGGPALGDPACR
                     DDLFGDGRLRLGHSGDAYGLRSGLWFDLEAGDGIAYFVTQVPEGQKGTRSAYSAAEEV
                     LVDTALRRSADASPLASPAHRH"
     gene            1294..1530
                     /locus_tag="VN034_02685"
     CDS             1294..1530
                     /locus_tag="VN034_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWV59533.1"
                     /translation="MSDSAEKVKKIVVEHLGVEADKVTEEASFIDDLGADSLDIVELV
                     MAFEEEFGVEIPDDAAEKIATVKDAIDYIEANKG"
     gene            1700..2962
                     /gene="fabF"
                     /locus_tag="VN034_02690"
     CDS             1700..2962
                     /gene="fabF"
                     /locus_tag="VN034_02690"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWV59534.1"
                     /translation="MRRVVVTGLGLVTPLGGDVETSWKNLIAGKSGAGTITHFDASDQ
                     KCTIACEVKGPDHEYGFDPGKRVDHKVQRQVDPFIIFGIDAAGQALEDAGLTDMTEEQ
                     KVRAGCSIGSGIGGLPGIESESLLLAEKGPGRVSPHFVHGRLINLISGQVSIKYGLKG
                     PNHAVVTACSTGAHSIGDAARMIRDDDADIMLAGGAEATICPIGIAGFAQARALNMTY
                     NDRPEQASRPYDKDRDGFVMGEGAGVVVLEEYEHAKARGAKIYAEVVGYGLSGDAYHV
                     TAPDPEMDGAFRSMAAALKKAGMTPADIDYINAHGTSTMADTIELGAVKRLFGDAMGN
                     VSMSSTKSAIGHLLGGAGAVETIFCILAIRDQIVPPTLNLDNPDEGTEGADLVPKVAK
                     KRKVKAALNNSFGFGGTNASLIVKAVED"
     gene            3084..4001
                     /gene="mltG"
                     /locus_tag="VN034_02695"
     CDS             3084..4001
                     /gene="mltG"
                     /locus_tag="VN034_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWV59535.1"
                     /translation="MVVIAALLLAACSGAPKDAEVVIPQGASIAKAGEILEDAGLVSA
                     SSFRNQARFFGSDEPIKPGEYKIEKGMDAGDILALFQSGKTIQRLVMIPEGMPSIMVW
                     ERLMAEPRLKGEIPVPAEGSILPDSYAFTTGETRAAVVKRMQAAMDKAFAELWAKRTP
                     RTAVKNRNEAITLASIVEKETAVAAERRTVAGVYTNRLGVGMRLQADPTIIYPVTKGK
                     PLGRRILRSEIQAVNGYNTYSMAGLPKGPIANPGKASIAAVLDPEANDYLFFVAQGDG
                     SHVFARTLSEHNANVQKWYALRRERGEME"
BASE COUNT          725 a         1304 c         1340 g          652 t
ORIGIN      
        1 gaaagcggcg ccttgacgct gcgccggggg ctgcgcagag tgacgccatg ctctcgcttt
       61 tagctctgct gacctcggtc gcatcgccgg ctcccgccgc ggtcgcggtg catttcaatc
      121 gcggcggaca ggtcgaggcg cgcgtcgtcg aggggcttgc cgaccgagca ggcggccgcg
      181 cgattacgcc cgacgatccg gtgcgcgtcg cgtcggtttc gaaactcgtc gtcgcgctcg
      241 gcgttctgcg gctcgtcgag gcgggcaagc tcgatctcga ccgcgacgtg tcggactatc
      301 tcggctggcg gctgcgccat cccgcttacc ccgaccggtc gatcagcctg tcgcagttgc
      361 tgagacatcg gtccgggctc aacgacgaaa ccaactatgc gctcccgctc gatgccgatc
      421 tcgaagccgc gcttcgcgac cccgccgctt gggacaaggc gcgtccaccg ggcggcgact
      481 tcgcctatgc caatttcaat tatcccgtcg tagcggccgc gatcgagggc gcaaccggcg
      541 agcggttcga ccaggcgatg acgcggctcg tttttcgtcc gctcggcctc gacgcctgtt
      601 tcaactggcc gacgtgcagc gacgcggcgg tcgcgcgtgc cgtcgtgctg cacgatgccg
      661 agggcaatgt cgtgcgcgac gatatgaagg gcgcgcggcc cgcctgtccg gtcgtcccgg
      721 caacgaacgg cagctgcgac ctcggcacct atcgcctcgc ccgccacggc gcggggttca
      781 gtccgcaggg cggactgcgt atttcggcga acgggctcgc gcgcatcggc gtgatgctgc
      841 tgcgccgcgg cgagggcttc ctgaaagccg aaagcctggc ccggctggag gcgatggcgc
      901 ccgtccggcc gaccagcggc gaggggacgg gcggcttctt ctgcgaatat ggcatggcga
      961 tgcacagcac cggcggcccg gcgctgggcg atccagcctg ccgcgacgat ctgttcggcg
     1021 atggacgcct gcggctcgga cattcgggcg atgcctatgg attgcgctcg ggcttgtggt
     1081 tcgaccttga agccggcgat ggcattgcct atttcgtcac gcaggtgccg gaggggcaga
     1141 aagggacacg gtcggcctat agcgctgcgg aggaagtctt ggtcgacacg gcgcttcgcc
     1201 gatcggccga tgccagcccc cttgccagcc ccgcccaccg gcactagggc aattttcaac
     1261 attcacgaac aggtcagaaa aaaggaactg tgcatgagcg attcggccga aaaggttaag
     1321 aagatcgtcg tcgaacatct gggcgtcgaa gccgacaagg tcaccgagga agcgagcttc
     1381 atcgacgatc tgggcgccga cagcctcgac atcgtcgaac tggtgatggc gttcgaagaa
     1441 gaatttggcg tcgaaatccc cgacgacgcg gcggaaaaga tcgccaccgt caaggatgcg
     1501 atcgactata tcgaagcgaa caagggctaa gcccctgttg tccggcgtgg ccggacgcgg
     1561 cggcgacgcc gccaacgcgt ttccggcttc ccggtcccga gcgctcgcgc gcagtggccg
     1621 ggaagctttt tttatggata tgcgtcttca aggatgacgc ggcaccctgc gggggcgggg
     1681 gccgatgaaa ggaatgatta tgcgccgggt tgtggtgacg ggtctgggtt tggtgacgcc
     1741 gctgggtggc gatgtcgaaa ccagctggaa gaatctgatc gcgggcaaat cgggtgccgg
     1801 cacgatcacc catttcgacg cgtcggacca gaaatgcacg atcgcgtgcg aggtcaaggg
     1861 tcccgaccat gaatatggat tcgaccccgg caagcgcgtc gaccacaagg tccagcgcca
     1921 agtcgacccc ttcatcatct tcggcatcga cgccgccgga caggcgctcg aggacgccgg
     1981 cctgaccgac atgaccgagg agcagaaggt gcgcgccggc tgctcgatcg gatcgggcat
     2041 cggcggcctg ccgggcatcg agagcgaaag cctgctgctc gccgaaaagg gcccgggccg
     2101 cgtgagcccg catttcgtcc acggccgcct gatcaacctg atctcgggtc aggtcagcat
     2161 caaatatggc ctcaagggtc cgaaccacgc cgtcgtcacc gcctgctcga ccggtgcgca
     2221 ctcgatcggc gatgcggcgc ggatgatccg cgacgatgac gccgacatca tgctcgcggg
     2281 cggcgccgag gcgacgatct gcccgatcgg catcgctggc ttcgcgcagg cgcgcgcgct
     2341 caacatgacc tacaacgacc gccccgaaca ggcgagccgc ccctatgaca aggaccgcga
     2401 cggctttgtg atgggcgagg gcgcgggcgt cgtcgtgctc gaggaatatg agcatgccaa
     2461 ggcgcgcggc gcgaaaatct acgccgaggt cgtcggttac ggcctgtcgg gcgacgccta
     2521 tcatgtcacc gcgcccgatc ccgagatgga cggcgcgttc cgctcgatgg cggccgcgct
     2581 caagaaggcg ggcatgaccc ccgccgacat cgactatatc aacgcgcacg gcacctcgac
     2641 gatggccgac acgatcgaac tcggcgcggt gaagcgcctg ttcggcgacg cgatgggcaa
     2701 tgtgtcgatg agctcgacca aatcggcgat cggccatctg ctcggcggcg cgggcgcggt
     2761 cgagacgatc ttctgcatcc tcgcgatccg cgaccagatc gtgcccccga cgctcaacct
     2821 cgacaatccc gacgaaggca ccgaaggcgc cgacctcgtc cccaaggtcg ccaagaagcg
     2881 caaggtcaag gccgcgctca acaacagctt cggtttcggc ggcaccaacg ccagcttgat
     2941 cgtgaaagcg gtcgaagact gatttttttc gtcatccccg cgcaggcggg gatccagcta
     3001 tggcgctcca tgccgaccgg acgaaagcgg gattcccgcc ttcgcgggaa tgacgaggag
     3061 agagacgcgt gtttcgctat ctgatggttg tgatcgcggc cctcctcctc gccgcctgct
     3121 cgggcgcccc gaaggatgcc gaggtcgtga tcccgcaagg cgcgagcatc gcgaaggcgg
     3181 gcgagattct cgaggacgcc gggctcgtct cggcgtcgag cttccgcaat caggcgcgct
     3241 tcttcggctc ggacgagccg atcaagcccg gcgaatataa aatcgaaaag gggatggacg
     3301 cgggcgacat tctcgcgctg ttccagtcgg gcaagacgat ccagcggctg gtgatgattc
     3361 ccgaaggcat gccctcgatc atggtgtggg agcggctgat ggccgagccg cgcctcaagg
     3421 gcgaaatccc cgtgcccgcc gagggcagca tattgcccga cagctatgcc ttcaccaccg
     3481 gcgagacccg cgccgcggtc gtcaagcgga tgcaggcggc gatggacaag gccttcgccg
     3541 agctttgggc caagcgcacc ccgcgcactg cggtcaagaa ccgcaatgaa gcgatcacgc
     3601 tcgcgtcgat cgtcgagaag gaaaccgccg tcgccgccga acgtcgcacc gtcgcgggcg
     3661 tctataccaa tcgccttggc gtcgggatgc ggctccaggc cgatccgacg atcatctatc
     3721 cggtcaccaa gggcaagccg ctcggccgcc gcatcctgcg ttccgaaatc caggcggtaa
     3781 acggctacaa cacctacagc atggccgggc tgcccaaggg gccgatcgcg aaccccggca
     3841 aggcgtcgat cgccgcggtg ctggaccccg aggcgaacga ctatcttttc ttcgtcgcgc
     3901 agggcgacgg cagccatgtt ttcgcgcgga cgctgtccga acataatgcc aatgtccaga
     3961 aatggtatgc gctccgccgc gagcgggggg agatggagta acgccgaact cttcaacgtc
     4021 g
//
LOCUS       DATMSU010000102         2938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_58868, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000102 DATMSU010000000
VERSION     DATMSU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58868"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..>2938
                     /gene="ileS"
                     /locus_tag="VN034_02700"
     CDS             116..>2938
                     /gene="ileS"
                     /locus_tag="VN034_02700"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWV59536.1"
                     /translation="MTDAPDYRDTVFLPKTDFPMKAGLPQKEPLILAKWLEANLEGQI
                     RESRKGRDQFILHDGPPYANGDMHIGHALNHILKDMVVRTQTLKGKDAPYVPGWDCHG
                     LPIEWKVEEQYRKKKLNKDEVPVEEFRAECRAYAQHWVDTQREQLKRLGIGGDWDHPY
                     LTMDFEAEATIVRELLKFAANDMLYRGAKPVMWSPVEKTALAEAEIEYEDIVSTQIDV
                     AFEIVESPIAELVGAHAVIWTTTPWTIPVNQALAYGPEVEYVVAEVDGRRHLVALSLL
                     VEFSRRAFPGKFGIQSQEDGTGVMNAPRVVWTGKGSDLAGTIARHPMHALGGFFARPR
                     PFLAGDFVTTDSGTGLVHMSPDHGEDDFDLCKANGLDPVFAVEGDGKYREDWGWLGGQ
                     GSVINPKFNAPDGPICTDLREAGALLAASADYKHSYPHSWRSKAKVIYRCTPQWFVPM
                     DKVMTHIEPKTPREKRWENEGGALNPHEEDLCDAPTLRQAAMQAIDDTRFVPAKGRNR
                     IGSMVEGRPDWVLSRQRAWGVPITLFVDRKTGHYLNDPAVNDRIVAAVKADGVDAWSD
                     ARAQEYLGDAYDAANYERITDILDVWFDSGCTHAFVLESGKWPALIRHDGSTHSADLY
                     LEGSDQHRGWFQSSLLESCGTRGQAPYKAVLTHGFTMDAKGFKMSKSLGNTISPIDLM
                     RDYGADILRLWALSVDFTEDHRIGKEILTGVADQYRKLRNTFRYLLGALDGFSEEERI
                     TDVAAMPELERYMLSLLADLDAKMAQAVDDFDYNSYTRLLADFCNEDLSAFYFDIRKD
                     VLYCDLGPAAPLGTDTRRAYRSVLDVLFHALVRYAAPVLVFTSEEVWGTRYPDAGSVH
                     LLEWPTVEAAWNDSSLVTKWAGVRTQREAVTEAIEPYRREKTIRSSLEAEVTIPEPAL
                     DPAALEEVFIVSVARAGDTLTVTRTEN"
BASE COUNT          565 a          988 c          906 g          479 t
ORIGIN      
        1 ggggttcaca aattgccagc ccccacgtga accccggcga aagccggggc ccaccgcacc
       61 cacacatgat cgtgaaggtt tgctcccgcc cgaatctgcg ctaagcgccc gggccatgac
      121 cgacgctccc gactaccgcg acaccgtctt cctgcccaag accgacttcc cgatgaaggc
      181 cggtctgccg cagaaggaac cgctgattct cgccaaatgg ctcgaagcca atctcgaggg
      241 gcagattcgc gagagccgca agggccgcga ccagttcatc ctccacgacg gacctcctta
      301 cgcgaatggc gacatgcaca tcggccatgc gctcaaccat atcctgaagg acatggtcgt
      361 ccgcacccag acgctgaagg gcaaggacgc gccttatgta cccggctggg actgccacgg
      421 cctgccgatc gagtggaagg tcgaggagca gtatcgcaag aaaaagctca acaaggacga
      481 ggttccggtc gaggagttcc gcgccgaatg ccgcgcctat gcgcagcatt gggtcgacac
      541 ccagcgcgag cagttgaagc gcctcggcat cggcggcgac tgggatcatc cgtatctgac
      601 gatggatttc gaagccgaag cgacgatcgt ccgcgaactg ctcaaattcg ccgcgaacga
      661 catgctctat cgcggcgcca agccggtgat gtggtcgccg gtcgaaaaga ccgcgctcgc
      721 cgaggccgag atcgaatatg aggatatcgt ctcgacccag atcgatgtcg cgttcgagat
      781 cgtcgagagc ccgatcgccg aactggtcgg cgcgcatgcg gtgatctgga cgaccacccc
      841 gtggacgatc ccggtcaacc aggctttggc ctatgggccg gaggttgagt atgtcgtggc
      901 ggaggtcgac ggtcgacgcc acttagttgc tctgtctcta ctggtggaat tttcccgccg
      961 ggcattcccg ggcaaatttg gcatccagtc gcaagaggat ggcacgggcg tgatgaacgc
     1021 gcctcgcgtc gtctggaccg gcaaaggctc cgacctcgcc ggcaccatcg cccgccaccc
     1081 gatgcacgcg ctcggcggct tcttcgcgcg tccgcgtccc ttcctcgcgg gcgatttcgt
     1141 caccaccgac agcggcaccg ggctcgtcca tatgtcgccc gatcacggcg aggacgactt
     1201 cgacctctgc aaggcgaacg ggctcgaccc cgtgttcgcg gtcgagggcg acggtaaata
     1261 tcgtgaggac tggggctggc tcggcggtca gggatcggtg atcaacccca agttcaacgc
     1321 ccccgacggc ccgatctgca ccgacctccg cgaagccggt gcgctgctcg ccgccagcgc
     1381 cgactataag cacagctatc cgcacagctg gcgctcgaag gccaaggtca tctatcgctg
     1441 caccccgcaa tggttcgtgc cgatggacaa ggtgatgacg catatcgagc ccaagacccc
     1501 gcgcgaaaag cgctgggaga atgagggcgg cgcgctcaac ccgcatgagg aggatctctg
     1561 cgacgcgccg acgctgcggc aggcggcgat gcaggcgatc gacgacacgc gcttcgtccc
     1621 cgccaagggc cgcaaccgca tcggtagcat ggtcgagggg cgccccgact gggtgctgtc
     1681 gcgtcagcgc gcgtggggcg tgccgatcac gctcttcgtc gaccgcaaga ccggccatta
     1741 tctcaacgac cccgccgtca acgaccgcat cgtcgcggcg gtgaaggcgg acggcgtcga
     1801 cgcatggtcg gacgcacgcg cgcaggaata tctcggcgac gcctatgacg ccgcgaacta
     1861 cgagcgcatc accgacattc tcgacgtctg gttcgattcg ggctgcaccc acgccttcgt
     1921 gctcgaaagc ggcaaatggc ccgcgctgat ccgccacgac ggatcgacgc atagcgccga
     1981 cctctatctc gaaggcagcg accagcatcg cggctggttc cagtcgtcgc tgctcgaaag
     2041 ctgcggcacg cgcggacagg cgccgtataa ggccgtcctc acccacggct tcacgatgga
     2101 tgccaagggc ttcaaaatgt cgaagtcgct cggcaacacg atcagcccga tcgacctgat
     2161 gcgcgactat ggcgccgaca tcctgcgcct gtgggcactg tcggtcgact tcaccgagga
     2221 ccaccgcatc ggcaaggaaa tcctcaccgg ggtcgccgac cagtatcgca agctcaggaa
     2281 caccttccgc tacctcttgg gcgcgcttga cgggttttcg gaagaggaac gcatcaccga
     2341 cgtcgcggcg atgcccgagc tcgaacgcta tatgctctcg ctgctcgccg acctcgacgc
     2401 gaagatggcg caggcggtcg atgatttcga ctataacagc tacacgcgcc tgctcgccga
     2461 tttctgcaac gaggacctct cggccttcta tttcgacatc cgcaaggacg tcctctactg
     2521 cgacctcggc cccgccgcgc cgctcggcac cgacacccgc cgcgcgtacc gcagcgtgct
     2581 cgatgtgctg ttccatgcgc tcgtccgcta cgccgcgccg gtgctggtgt tcaccagtga
     2641 agaggtatgg ggcacgcgct atcctgacgc gggcagcgtg catttgctcg aatggccgac
     2701 ggtcgaggcc gcatggaatg acagctcgct cgtgacgaaa tgggcgggtg tgcggaccca
     2761 gcgcgaggcg gtgaccgagg cgatcgaacc ctatcgccgc gaaaagacga tccggtcgag
     2821 ccttgaggcc gaagtcacga tccccgaacc tgcgctcgac cccgcagcgc tcgaagaagt
     2881 gttcatcgta tcggtcgcgc gcgcgggcga taccctcacc gtcactcgca ccgaaaac
//
LOCUS       DATMSU010000103        14339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_11401, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000103 DATMSU010000000
VERSION     DATMSU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 14339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14339
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11401"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..865)
                     /locus_tag="VN034_02705"
     CDS             complement(86..865)
                     /locus_tag="VN034_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWV59537.1"
                     /translation="MPDLASLAERLDIAARTASATTQLTAAHPDLTLDDAYAIQHRLI
                     AHREARGERVIGMKMGFTSRAKMAQMGVSDLIWGRLTDAMQIADDGALDLRRFVHPRI
                     EPEVAFRLKAPLAGPVGSAEALAAIEAVAPAMEVIDSRYADFRFALEDVVADNSSSSA
                     FVVGSWQAPFGDLSNLGMVMSFDGRPVQFGSSGAILGNPLRSLVEAARLAGETGVTLE
                     AGWIILCGAATNAEALRPGVHVRLEAERLGRVDLNILDEGA"
     gene            complement(865..1908)
                     /gene="dmpG"
                     /locus_tag="VN034_02710"
     CDS             complement(865..1908)
                     /gene="dmpG"
                     /locus_tag="VN034_02710"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HWV59538.1"
                     /translation="MTKTFNVEAGDKLYIQDVTLRDGMHAIRHMYGIDDVKAIAKALD
                     DAGVDAIEVAHGDGLSGASFNYGFGAHTDWEWLEAVASVLTKSVLTTLILPGVGTVEE
                     LRRAYDLGVRSVRVATHCTEADVSKQHIGIARDLGMDVAGFLMMSHMIEPEALAQQAL
                     LMESYGAECVYVTDSGGALDMDGVKARLEAYDRVLKPETQRGIHAHHNLSLGVANSIV
                     AAQCGAVRIDASLTGMGAGAGNAPLEVFIAAADRKGWKHGCDLMALMDAAEDLVRPLQ
                     DRPVRVDRETLCLGYAGVYSSFLRHAEKAAADHGLDTRAILVELGRRRMVGGQEDMIT
                     DVALDMVRQKESL"
     gene            complement(1905..2849)
                     /locus_tag="VN034_02715"
     CDS             complement(1905..2849)
                     /locus_tag="VN034_02715"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HWV59539.1"
                     /translation="MTRVKAAIIGSGNIGTDLMIKMIKYPQNMELVAVVGIDEKSEGL
                     AMARERGVATTHEGIDGLKAMDVYPEIGIVFDATSAYAHKVHDAALRADGKQVIDLTP
                     AAIGPYTIPTVNGDANLDAGNVNMVTCGGQATIPMVAAVSQVATVHYAEIVASVSSRS
                     AGPGTRANIDEFTRTTAGAIEKVGGAAQGKAIIILNPAEPPMIMRDTVFTLSEGADED
                     TIRASVEAMVKKVQAYVPGYRLKQEVQFERFGDNNKLRIPGRGEFTGIKTMILLEVEG
                     AGDYLPSYSGNLDIMTAAAKATGELLAERIREGKKVTA"
     gene            complement(2859..3647)
                     /locus_tag="VN034_02720"
     CDS             complement(2859..3647)
                     /locus_tag="VN034_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWV59540.1"
                     /translation="MSIAETYGRELYAALRDRRTVAPLIARDASLTIDDAYAISLDFL
                     SRRLKDGERVVGKKIGVTSKAVQDMLGVHQPDFGFLTDAMHVEGDIDIDAKALIAPRA
                     EAEIAFILKDSLNGPGVTAADVIAATASIAPCFEIVDSRITDWKIGIVDTVADNASCG
                     VFVLGKERIDPRALDLPNLHVAVAKNGSPLSEGYGHAVQGDPAQAVAWLANTLGAHGV
                     TLDAGDIILSGSLVPLAPAVKGDIFEMILGDGRRTLGHCVARFV"
     gene            complement(3634..4452)
                     /locus_tag="VN034_02725"
     CDS             complement(3634..4452)
                     /locus_tag="VN034_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV59541.1"
                     /translation="MGEMKETTHRVAGGYDIHIKEAGEGPALVFVHGSGPGASGLSNF
                     RQNVDAFVAAGYRVILPDLIGYGASSKPADIDYTLTLFTDTLYDALKAHGIESATLIG
                     NSLGGGVAIQMTQDHPEFVERLILMAPGCIEELDVYFAMPGIADMRSSFGSPDFSLAD
                     QRRLGESLVYDPKHITDALVEERFAVASQQPKEVITRMRTHNVRPRLGEIKVPILLFW
                     GQNERFMPLSGIQYFFEACDDVRCVTFNKVGHWVQVERASEFNRYAIEFLDEHR"
     gene            complement(4465..5676)
                     /locus_tag="VN034_02730"
     CDS             complement(4465..5676)
                     /locus_tag="VN034_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV59542.1"
                     /translation="MATAPQQAADTALPTPGALIERARAMIPTLKARARQTTLDGNVS
                     AETVAEMQEAGFFRILQPKRWGGYEMHPNVFFEVQKALAEGCMSTGWIYGVLGCHPYE
                     LALFHDEAQREVWGDNGGSMLVSSTYQPVGKVEKVEGGFYLSGRWGFSSGSTHCGWVL
                     LGALNFDTDGGPPDMRTFLLPRSDYQVIEGTWDVFGLQGTGSFDILVERVFVPEHRTH
                     RASDGFACTNPGQAENDGPLYRLPWAQIFVRSVSTAAFGGARAAVNAAMEIMQGRIST
                     NTGKASKADPLLHGAIAKAHAQILEMELTHQTTFDRLMAKSESGEAITLEERALYAYQ
                     SSTVVRRLAELVDGMVQLLGGRAIYMSSAIIQPWLDLNAARAHVANDPGNRTTDVVMT
                     MLGEPPAFTFV"
     gene            6055..6945
                     /locus_tag="VN034_02735"
     CDS             6055..6945
                     /locus_tag="VN034_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59543.1"
                     /translation="MAIRSLAYVVVDTADIEAWRAYAEQVLGMQAIDHAGGLRLRMDE
                     RPFRMLIRKGDAEQFHAAGLVYPDKAAFDRGVAGLRDAGVAVEPASEDEAKDRHVVEF
                     VRCADPSGNRLELGWGRVVDGNPFVSPAGVSEFITGEMGMGHVVFPAAKLAETRAFYI
                     DLLGFGDTDEMRVYMQGGGPDDPGLGLHFLHCDNPRHHSVALAEFPVPSGLVHMMVEV
                     PGLDDVGRALDRAKAANVHISSSLGKHMNDKMVSFYMRSPGGFDIEYGFGGIQPDWTS
                     FTPTISLKEDEWGHHWDFGG"
     gene            6993..7370
                     /locus_tag="VN034_02740"
     CDS             6993..7370
                     /locus_tag="VN034_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59544.1"
                     /translation="MPSHDDMVRAVERYVEAFDKGDAAMASGLYADDATVEDPIGSPI
                     HQGRDAIHAFYSMSMQAGAKLRLDGPVRTGGDYAAFAFTVLVDFGGPKEIEVIDTFKF
                     DAEGKVTEMRAYWSPANMRAVEA"
     gene            7372..8577
                     /locus_tag="VN034_02745"
     CDS             7372..8577
                     /locus_tag="VN034_02745"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWV59545.1"
                     /translation="MAREAAIVSTARTGVGKAYRGAFNDTEAPVLAAHVVNAAIARAG
                     IDPARVDDVYLGVGNHWNTQSYNLGRLAVHGSVLPDSTGGFTLDRKCSSGLNALALAA
                     RGIMCNEIDVAVAGGMESISLTLNKHAPLFRNRSEFIKAADPHAYMAMIETAEIVADR
                     YGVSREAQDAFAANSQQRAGAAQAAGKFADEIVPITVQKALFDKEGNETGKEDVTLAH
                     DEGVRGDTTAEKLAGLKPVFKDGLVVKESRHVTAGNASQLSDGASAQVVMDRAMAEKE
                     GLPILGLYRGFQVAGCKAEEMGIGPVFAVPKLLDRAGLKVDDIGLWEINEAFASQALY
                     CRDKLGIDPDRLNVNGGGIAIGHPFGMTGSRLVGHALIEGKRRGVRYAVVSMCVAGGM
                     GAAGLFEIV"
     gene            8595..9350
                     /locus_tag="VN034_02750"
     CDS             8595..9350
                     /locus_tag="VN034_02750"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWV59546.1"
                     /translation="MALLFEGKTAFVTGGGSGIGRAAALAFAAEGAKVAIGDLNGDSA
                     AETAALIRGEGGEAHHGALDVTDAGAVDAFVDDVVQRWGRLDCAFNNAGVSLEGMETP
                     WGDLAAYDRSIAVNQRGLLLCMAAELRHMEKAGRGAIVNTASIAGMSGAGGAGYCGSK
                     HAVIGLTRSAAMRYAAQGIRINAVCPGAIRTPMVDAVVKEEAAARFVAEMHPMNRIGE
                     AHEIADAVIFLCSDKASFITGHPLAVDGGYLAR"
     gene            complement(9536..11221)
                     /locus_tag="VN034_02755"
     CDS             complement(9536..11221)
                     /locus_tag="VN034_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV59547.1"
                     /translation="MAVHEVDILVIGSGAGALTAAFTAADAGAEVLVVEKSARYGGTS
                     ATSGGGIWIPNSDDARAKGHADDPEEALGYIKALIGDDVPETKLRAYVDNAHRMLSYL
                     EERSQVRYTAYAYSDYHMDVAGARDGWRTHDPIPLMADRLGADFERMEPPHTLTLTFG
                     RFTWTMAEAKLLLTMSPGAKPLLFKLMLKYMFDLPWRFKTKRSRRLTGGNALIGRLKL
                     SIDEKGVPIWLNAPMVELVRDKSGIAGAIVERDGQRIEVRARRGVIIASGGFDHDAAL
                     RETYLPQPTSADWSAGVPSNTGDGLKAGLAVGAATALMDSAWWGPGFLLEGEDRARIL
                     FVERALPSSIIVNQAGKRYMNEAASYHVAGGEMHRCNTPEAPTAPSWFVFDARYRGKY
                     MLGPMMAGKPSADSRIPEAMRKILKKSATIAGLAKEIGVDAAVLSKEIARFNANALKG
                     EDADFGRGASAYDRHYGDASVTPNPTLAPIAEAPFYAIPIYPCDLGTNGGLLTNDNGQ
                     VLGEDGKPLGGLYAIGNASAAAMGRTYPGAGATLGPAMTFGWLAARHATRVNE"
     gene            11444..11965
                     /locus_tag="VN034_02760"
     CDS             11444..11965
                     /locus_tag="VN034_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV59548.1"
                     /translation="MRTATKAGKRTRGKAANASQGDGMLALDDVDEQIIAALRKNGRI
                     ANRDIAREMGLNEATVRTRLRRLEDARMVRVVAMRDLAAMGFGYLAPVGVQVKGRPAG
                     DVGAEIAELERVITVNVAIGTHDLEIQVVAETLEEMQHLLTGVIAKIDGVERIFPSLA
                     LKVFKYNPEWAPL"
     gene            11962..12432
                     /locus_tag="VN034_02765"
     CDS             11962..12432
                     /locus_tag="VN034_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV59549.1"
                     /translation="MSTRISLDDLDHKIIERLGHDARVSNREIGREFDLTEGTIRSRL
                     KRLLDNRVIRVAAVTNANRLRNPILAYLWVEADSAADIERVAESLAKLPEIGFVATTL
                     GRADVLAMTLVENGNELTDFLHQTIDKIPGVRRVRYSLGQNFIKHDYRWCALVD"
     gene            complement(12665..13132)
                     /locus_tag="VN034_02770"
     CDS             complement(12665..13132)
                     /locus_tag="VN034_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59550.1"
                     /translation="MTELESRIARLEAESDIRRLKARYLNACDAKDVGAIRACFTEDA
                     VIDFPPIGEFGLDGLIDVFTQMAATTPIVDVHQGHNAEIEVTGDEASARWNLGYATYD
                     PRAGSFRLLANFYHDRYRRTPDGWRICYTRSEPRAIVDGVLAADGIKAEWVAE"
     gene            complement(13119..14207)
                     /locus_tag="VN034_02775"
     CDS             complement(13119..14207)
                     /locus_tag="VN034_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HWV59551.1"
                     /translation="MTEGSILSPDVWRDFCRRLEAAGEIITSAEAAANPLDRAEGYRY
                     LTRLLRIALDMQLEHADPDFPSFYAASHETAKIGADNPDNIYMNATVSGARRYRIRGQ
                     RGSVPILSFGTKANRYAIDGTMASTGELDSADMAIAPDGSFEIIVSRERAEGNWLPLA
                     KDSSMVLVRQTFLDRPNEIPASVTIEALDGPTYPAPLSPDGLEAALERVTGFVAGTAR
                     TFVQWAEDFRAEQMNRLATVDQTRFFRAGGDPTIFYLHGWWQLEPGEALQIDTPIPAC
                     SFWNFQLDNVWMESLDYRHHRIHVNGHSARLNDDGTLTLVVAPRDPGFGNWIDTAGHH
                     QGTMLLRWIGATDHPIPETRIIPIDDRA"
     gene            complement(14210..>14339)
                     /locus_tag="VN034_02780"
     CDS             complement(14210..>14339)
                     /locus_tag="VN034_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HWV59552.1"
                     /translation="IPLGVIPPEEDCAKSVLFFLSDYAKMVTGATLDVNGGQYMAP"
BASE COUNT         2455 a         4586 c         4730 g         2568 t
ORIGIN      
        1 gatacgaaca ggaaatcgcc ggcacggcga tagtgcggaa aggcgccgcg cggcacggcc
       61 ttgcccggaa tgacgcggcc gccggtcatg cgccttcgtc caatatgttg aggtcgacac
      121 ggccgagccg ctcggcttcg agccggacat gcacccccgg tcgcagcgcc tcggcattgg
      181 tggccgcgcc gcacaggata atccagcccg cttcgagcgt gacgcccgtc tctccggcga
      241 gacgcgccgc ctcgaccagc gaacgcagcg ggttgccgag gatcgcgccg ctcgagccga
      301 actggacggg gcgcccgtcg aagctcatca ccatgccgag gttcgagagg tcgccgaacg
      361 gtgcctgcca gctgccgacg acaaaggccg acgaggagct gttgtcggcg acgacgtctt
      421 cgagcgcgaa acggaagtcg gcatagcggc tgtcgatcac ttccatcgcc ggtgcgaccg
      481 cctcgatcgc cgccagcgcc tcggccgaac cgaccggtcc ggcgagcggt gccttgagac
      541 ggaaggcgac ctcgggctcg atccgcggat ggacgaagcg gcgcaggtcg agcgcgccgt
      601 cgtcggcgat ctgcatcgca tcggtcagcc gaccccagat caggtcggaa actcccatct
      661 gcgccatctt ggcgcggctg gtgaagccca tcttcatccc gatgacccgt tcgccgcgcg
      721 cttcgcgatg cgcgatcagc cgatgctgga tcgcataggc gtcatcaagc gtgagatcgg
      781 gatgcgcggc ggtaagctgc gttgtggcgg atgcggttcg ggctgcaatg tccagccgtt
      841 cggcgaggga ggcgaggtcg ggcatcacag gctttccttc tgccggacca tgtccagcgc
      901 tacgtcggtg atcatgtctt cctgtccgcc gaccatgcgg cggcggccga gttcgacgag
      961 gatggcgcgg gtatcgaggc cgtgatcggc ggccgccttt tcagcgtggc gcaggaagct
     1021 cgaatagacg ccggcatagc cgaggcacag cgtctcgcgg tcgacccgga ccggacgatc
     1081 ctgcagcggc cggacgagat cctcggcggc gtccatcaac gccatcaggt cgcaaccgtg
     1141 cttccagccc ttgcggtcgg cggcggcgat gaacacctcg agcggcgcgt tgcccgcgcc
     1201 cgcgcccatc ccggtgaggc tggcgtcgat gcgcacggcg ccgcactgcg ccgcgacgat
     1261 gctgttcgcg accccgagcg acagattgtg gtgcgcgtgg atgccgcgct gcgtttcggg
     1321 tttgagcacg cggtcatagg cttcgagccg cgccttcacc ccgtccatgt cgagcgcgcc
     1381 gcccgagtcg gtgacataga cgcattcggc gccatagctt tccatcagca aggcctgctg
     1441 cgcgagcgcc tcgggctcga tcatatggct catcatcagg aagcccgcga catccatgcc
     1501 aagatcgcgc gcgatgccga tatgctgttt cgacacgtcg gcctcggtgc aatgcgtcgc
     1561 gacgcgcacc gaacggacgc cgaggtcata ggcgcggcga agttcctcga ccgtgccgac
     1621 gccgggcagg atcagcgtgg tgagcaccga cttcgtcagc accgaagcga ccgcctccag
     1681 ccattcccag tcggtgtgcg cgccgaagcc atagttgaag ctcgcgccgc tcaggccgtc
     1741 gccatgcgcg acctcgatcg cgtcgacccc cgcgtcgtcg agcgccttcg cgatcgcctt
     1801 cacatcgtcg atgccgtaca tatggcggat cgcgtgcatt ccgtcgcgga gtgtgacgtc
     1861 ctggatatag agcttgtcgc ccgcctcgac gttgaatgtc ttcgtcatgc ggtcaccttc
     1921 tttccttcgc ggatacgctc cgcgagcagt tcgccggtcg ccttggcggc ggcggtcatg
     1981 atgtcgagat tgcccgaata gctgggcagg taatcgcccg cgccctcgac ctcgagcagg
     2041 atcatcgtct tgatgcccgt aaactcgccg cgcccgggaa tcctgagctt gttgttgtcg
     2101 ccgaagcgct cgaactgcac ctcctgtttc aggcgatagc cgggcacata ggcctgcacc
     2161 ttcttcacca tcgcctcgac gctggcgcgg atcgtgtcct cgtcggcgcc ttcggacaaa
     2221 gtgaacacgg tgtcgcgcat gatcatcggc ggttcggcgg gattgaggat gatgatcgcc
     2281 ttgccctgcg ccgcgccgcc gaccttttcg atagctccag cagttgtgcg ggtgaattcg
     2341 tcgatattgg cgcgcgtgcc gggcccggcc gagcgcgacg acaccgatgc gacgatctcg
     2401 gcataatgga cggtcgcgac ctggctcact gcggcgacca tcgggatcgt cgcctgtccg
     2461 ccgcacgtca ccatattgac gttgcccgca tcgagattgg cgtcgccgtt gacggtcggg
     2521 atcgtatagg ggccgatcgc ggcgggggtc aggtcgataa cctgcttgcc gtcggcgcgc
     2581 agcgccgcgt catgcacctt gtgcgcatag gccgaggtcg cgtcgaagac gatgccgatc
     2641 tcgggataga cgtccatcgc cttgagcccg tcgatgcctt cgtgtgtcgt cgcgacgccg
     2701 cgttcgcgcg ccatcgcgag accttcggat ttctcgtcga tgccaacgac cgcgaccagt
     2761 tccatattct gcggatattt gatcatcttg atcatcaggt cggtgccgat attgcccgaa
     2821 ccgatgatcg ctgccttcac gcgggtcatg ggtgttcctt atacaaaacg cgcgacgcaa
     2881 tggccgagcg tgcggcggcc gtcgccgagg atcatttcga agatgtcgcc ctttaccgcc
     2941 ggtgcaagcg gaacaaggct gcccgacagg atgatgtcgc ccgcgtcgag cgtcacgccg
     3001 tgcgcgccga gcgtattggc gagccacgcg accgcctgcg ccgggtcgcc ctgaaccgca
     3061 tggccatagc cttcggaaag cggcgatccg ttcttcgcga ccgcgacatg caggttcggc
     3121 agatcgagcg cgcgcggatc gatccgttcc ttgccgagca cgaagactcc gcacgaggcg
     3181 ttgtcggcaa ccgtgtcgac aatgccgatc ttccagtcgg tgatgcggct gtcgacgatc
     3241 tcgaaacagg gggcgatgct cgcggtcgcc gcgatcacgt cggccgcggt cacgccggga
     3301 ccgttcaggc tgtccttcag gatgaaggcg atctcggctt cggcgcgcgg cgcgatcagc
     3361 gctttcgcgt cgatgtcgat atcgccctcg acatgcatcg cgtcggtgag gaagccgaaa
     3421 tcgggctggt ggacgcccag catgtcctgc accgcctttg acgtgacgcc gatcttcttg
     3481 ccgacgacgc gctcgccgtc cttcagccgc cgcgacagga agtcgaggct gatcgcatag
     3541 gcgtcatcga tcgtcagcga ggcatcgcgc gcgatcagcg gtgcgacggt gcggcggtcg
     3601 cgcagcgccg catagagttc gcggccgtaa gtttcagcga tgctcatcga ggaactcgat
     3661 ggcatagcgg ttgaactcgc tcgcgcgttc gacttgcacc cagtggccga ccttgttgaa
     3721 ggtcacgcag cgcacgtcgt cgcacgcttc gaagaaatat tggatgcccg agagcggcat
     3781 gaagcgctcg ttctggcccc agaagagcag gatcgggacc ttgatctcgc cgagccgcgg
     3841 ccggacatta tgcgtccgca tccgcgtgat tacttccttg ggctgctggc tcgcgaccgc
     3901 gaaacgctcc tcgaccagcg cgtcggtgat gtgcttcggg tcgtagacca gactttcgcc
     3961 gaggcggcgc tggtcggcga ggctgaaatc ggggctgccg aacgaggatc gcatgtcggc
     4021 gatgccgggc atggcgaaat aaacgtcgag ttcctcgatg cagccgggcg ccatcaggat
     4081 caggcgctcg acaaattcgg gatggtcctg cgtcatctgg atcgccacgc cgccgcccaa
     4141 tgaattgccg atcagcgtcg cgctctcgat cccgtgcgcc ttcagagcgt cgtacagcgt
     4201 gtcggtgaac aaggtcagcg tataatcgat gtccgcgggt ttcgacgagg cgccatagcc
     4261 gatcaggtcg ggcaggatca cgcggtagcc ggccgcgacg aacgcatcga cattctgccg
     4321 gaaattggac agccccgatg cgccggggcc gctgccgtgg acgaacacca gcgccgggcc
     4381 ttcgcccgct tccttgatat ggatgtcata tccgcccgcg acgcggtgcg tcgtttcctt
     4441 catctctccc atggccgttt cctcttacac gaatgtgaag gcgggcggtt cgccgagcat
     4501 cgtcatcacg acgtcggtgg tgcgattgcc ggggtcgttg gcgacgtgcg cgcgcgccgc
     4561 attgaggtcg agccacggct ggatgatcgc gctcgacata tagatggcgc ggccgccgag
     4621 gagctggacc atgccgtcga ccagctcggc gagccgccgc acgacggtcg agctctgata
     4681 ggcatagagc gcgcgttctt cgagcgtgat cgcctcgccg ctttcggatt tcgccatcag
     4741 ccggtcgaag gtcgtctggt gcgtcagctc catctcgagg atctgcgcat gcgccttggc
     4801 aatcgcgcca tggagcaaag gatcggcctt cgacgccttg ccggtgttgg ttgagatgcg
     4861 gccctgcatg atctccatcg cggcgttcac cgccgcgcgg gcgccgccga acgccgcggt
     4921 cgacaccgag cggacgaaga tctgcgccca gggcaggcga tagagcgggc cgtcattctc
     4981 ggcctggccg ggattggtgc aggcgaagcc gtcgctggcc ctgtgcgtgc gatgttcggg
     5041 gacgaagacg cgctcgacga gaatgtcgaa gctgccggtg ccttgcagcc cgaagacgtc
     5101 ccacgtcccc tcgatgacct gatagtcgct gcgcgggagc aggaaggtcc gcatgtcggg
     5161 cgggccgccg tcggtgtcga agttcagcgc gccgagcagc acccagccac aatgcgtcga
     5221 acccgacgaa aaaccccagc ggcccgacag gtaaaagccg ccctcaacct tctcgacctt
     5281 gcccaccggc tgataggtcg acgaaacgag catcgaaccg ccattatcgc cccacacctc
     5341 gcgctgcgcc tcgtcgtgga aaagcgcgag ttcatagggg tggcagccga gcacgccgta
     5401 aatccagccg gtcgacatgc agccttcggc gagcgccttc tgcacctcga agaagacatt
     5461 ggggtgcatt tcatagccgc cccagcgttt cggctggagg atgcggaaga atcccgcttc
     5521 ctgcatctcg gcgacggttt cggcggacac attgccgtcg agcgtcgtct ggcgcgcgcg
     5581 cgccttcagt gtcgggatca tcgcgcgcgc gcgctcgatc agtgcgccgg gggtgggcag
     5641 cgcggtgtcg gcggcttgct gcggggcggt ggccatatgc tctcctcggg atatttatgc
     5701 gcttgcggga ggccgcgtcg cgatatcact cctgtattcg tagtgaatga tgcatttctc
     5761 tacgcaagct gtcaatccgg attctatgca aaatgtcgct ttgggccatt cgaaggggcg
     5821 ggctgcgtat cgaggcgcgc aacgcgatac gcaaagtgtc gcttgccgta gcggcgttca
     5881 ggactctgga ttggtcgatc gatagcgata agtgcgattt gagaaagcaa aatatacggt
     5941 ttgcgtaaat tttgacgcaa atttctacgt atcatacttg atgtgaggcg tattcgcttg
     6001 tagcttgcgc tcgattcaag ggtgccggag cacccgtccg taggggatga gtgaatggca
     6061 attaggtcac tggcctatgt cgtggtcgat acggccgata tcgaagcgtg gcgtgcctat
     6121 gccgagcaag tgctgggtat gcaggcgatc gaccatgcgg gcgggctccg cctgcgcatg
     6181 gacgagcgcc ccttccgcat gctgattcgc aagggcgacg ccgagcagtt tcacgccgcg
     6241 gggctcgtct atcccgacaa ggcggccttc gatcgcggcg ttgcggggct ccgcgatgcg
     6301 ggtgtcgcag tcgagcccgc gtccgaggac gaagcgaagg atcgccatgt cgtcgagttc
     6361 gtccgctgcg ccgatccctc gggcaaccgg ctcgaactcg gctgggggcg cgtcgtcgac
     6421 ggcaacccgt tcgtctcgcc cgcgggcgta tccgaattca tcacgggcga gatggggatg
     6481 gggcatgtcg tcttccccgc ggccaagctc gcggaaacgc gcgctttcta tatcgacctg
     6541 ctcggcttcg gcgacaccga cgagatgcgc gtctatatgc agggcggcgg acccgacgat
     6601 ccggggctcg gcctccattt cctccactgc gacaatccgc gccaccatag cgtcgcgctc
     6661 gccgagtttc cggtaccgag cgggctcgtt cacatgatgg tcgaggtgcc gggcctcgac
     6721 gatgtcggcc gcgcgctcga tcgtgcgaag gcggcgaacg tccatatctc gtcgagcctc
     6781 ggcaagcaca tgaacgacaa gatggtcagc ttctacatgc gcagccccgg cggcttcgac
     6841 atcgaatatg gctttggcgg catccagccc gactggacca gcttcacccc gacaatcagc
     6901 ctcaaggaag acgaatgggg tcatcattgg gatttcggcg gctgacgcgc cgtcgtcatc
     6961 ctgccccctt cctgcatcat tggagagata ccatgcccag ccatgacgat atggtccgcg
     7021 ccgtcgagcg ctatgtcgag gcgttcgaca agggtgacgc cgcgatggcg tccggccttt
     7081 acgccgacga cgcgacggtc gaggatccga tcggctcccc gatccatcag gggcgcgacg
     7141 cgatccacgc cttctattcc atgtcgatgc aggcgggcgc caagctccgt ctcgacggcc
     7201 ccgtgcgcac cggcggcgat tatgcggcgt tcgccttcac cgtgctcgtc gactttggcg
     7261 gacccaagga gatcgaagtg atcgacacct tcaaattcga tgccgagggc aaggtgaccg
     7321 agatgcgcgc ctattggagc cccgccaaca tgcgcgcagt cgaggcctga gatggcacgc
     7381 gaagccgcca tcgtttcgac cgcgcgcacc ggggtcggca aggcctatcg cggtgcgttc
     7441 aacgacactg aggcaccggt gctggcggcg catgtcgtca acgccgcgat cgcgcgcgcc
     7501 gggatcgatc cggcacgcgt cgacgacgtc tatctgggcg tcggcaatca ctggaacacc
     7561 cagagctaca atctcggccg gctggcggtg cacggttcgg tgttgcccga cagcacgggc
     7621 ggctttacgc tcgaccgcaa atgttcgtcg gggctcaacg cgctcgcgct cgccgcgcgc
     7681 gggatcatgt gcaacgagat cgacgtcgcg gtggccgggg ggatggagag catttcgctg
     7741 acgctgaaca agcacgcgcc gctgttccgc aaccgttcgg aattcatcaa ggccgccgac
     7801 ccccacgctt atatggcgat gatcgagacc gctgagatcg tcgccgaccg ctatggcgtc
     7861 agccgcgagg cgcaggatgc gttcgccgcg aacagccagc agcgcgcggg tgcggcgcag
     7921 gccgcgggaa agttcgccga cgaaatcgtg ccgatcaccg ttcaaaaagc gctgttcgac
     7981 aaggagggca atgagaccgg caaggaagat gtcacgctgg cgcacgacga gggcgtgcgc
     8041 ggcgacacca ccgccgaaaa gcttgcgggg ctcaagcccg tgttcaagga cgggctggtc
     8101 gtcaaggaaa gccgccatgt caccgcgggc aacgccagcc agctgtccga cggcgcctcg
     8161 gcgcaggtcg tcatggaccg cgcgatggcc gagaaggagg ggctgccgat cctagggctt
     8221 tatcgcggtt ttcaggtcgc gggctgcaag gccgaggaaa tgggcatcgg cccggtcttc
     8281 gccgtcccca agctgctcga ccgcgccggc ctcaaagtag acgacatcgg cctctgggaa
     8341 atcaacgagg cgttcgcgag ccaagcgctc tattgccgcg acaagctcgg catcgatcca
     8401 gacaggctca acgtcaacgg cggcggcatc gcgatcggcc atccgttcgg gatgacgggc
     8461 tcgcgcctcg tcggccacgc gctgatcgag ggcaagcgcc gcggcgtccg ctatgcggtc
     8521 gtctcgatgt gcgtcgcggg cggcatgggt gccgcgggcc tgttcgaaat cgtctgaagg
     8581 caaaggaggc agcgatggcg ctgttgttcg aaggaaaaac cgctttcgtc accggcgggg
     8641 gcagcggcat cggccgcgcc gccgcgctgg cctttgccgc cgagggcgcc aaggttgcga
     8701 tcggcgatct caatggcgac agcgctgccg aaaccgccgc gctgatccgc ggcgagggcg
     8761 gcgaggcgca tcatggcgcg ctcgacgtca ccgacgcggg cgcggtcgat gcgtttgtcg
     8821 atgatgtcgt gcagcgctgg ggacggctcg attgcgcctt caacaatgcc ggggtttcgc
     8881 tcgagggaat ggaaacgccg tggggcgacc tcgccgctta tgatcgcagc atcgcggtca
     8941 atcagcgcgg gttgctgctc tgcatggcgg ccgaactccg ccatatggaa aaggcggggc
     9001 gcggcgcgat cgtcaacacc gcgtcgatcg cgggcatgtc gggcgccggg ggcgcgggct
     9061 attgcgggtc gaaacatgcg gtgatcgggc tcacgcgctc ggcggcgatg cgctacgccg
     9121 cgcagggcat ccgcatcaac gcggtctgcc ccggcgcgat ccgcacgccg atggtcgatg
     9181 cggtggtgaa ggaagaagcc gcggcgcgct tcgtcgccga aatgcacccg atgaaccgca
     9241 tcggcgaagc gcatgaaatc gccgacgcgg ttatcttcct ctgctcggac aaggcgagct
     9301 tcatcaccgg gcatccgctc gcggtcgacg gcggctatct ggcgcgctga gcaagcagcc
     9361 gtcaccgggc tttcatcggc gtgacggcta tggggtgggg aaccgccgtt gtgtaccccg
     9421 gcgaaagccg gggcccagtg cggaaaagcg caaggttgca ttggaattct aggccccggc
     9481 tttcgccggg gttcacatct ctatcggtag caaccggtcg cttcccgacg taggtttatt
     9541 cgttcacccg cgtcgcatgc cgcgctgcaa gccagccgaa ggtcatcgcc gggccgagcg
     9601 ttgcgcccgc gcccggatag gtccgcccca tcgccgccgc cgaggcgttg ccgatcgcat
     9661 agagcccgcc gagcggcttg ccatcctcgc cgagcacctg accattgtcg ttggtgagca
     9721 gtccgccgtt ggtgccgaga tcgcacggat agatcgggat cgcatagaag ggcgcttcgg
     9781 cgatcggcgc gagcgtcgga ttcggcgtca ccgacgcatc gccataatgc cggtcatagg
     9841 cggaggcgcc gcggccgaaa tcggcatcct cgcccttgag cgcattggcg ttgaagcgcg
     9901 caatttcctt ggaaagcacc gcggcgtcga cgccgatctc ctttgcgagc ccggcaatcg
     9961 tcgcggattt cttcaatatc ttgcgcatcg cctcggggat gcggctgtcg gccgatggct
    10021 tgcccgccat catcgggccc agcatatatt tgccgcgata gcgcgcgtcg aatacgaacc
    10081 aggagggggc ggtgggcgcc tcgggggtgt tgcagcggtg catctcgccc cccgcgacat
    10141 gataggatgc cgcctcgttc atataacgct tgcccgcttg gttgacgatg atcgacgagg
    10201 ggagcgcgcg ctcgacgaac aggattcgcg cccggtcctc gccctcgagc aggaagccgg
    10261 gaccccacca ggccgaatcc atcagcgcgg tcgcggcccc caccgcgagt cccgccttca
    10321 gcccgtcgcc ggtattgctc ggcacccccg cgctccagtc ggccgaggtc ggttgcggca
    10381 gatatgtctc gcgcagcgcg gcatcatggt cgaagccgcc gctcgcaatg atcacgccgc
    10441 ggcgcgcgcg aacctcgatc cgctggccgt cgcgttcgac gatcgcaccg gcaattccgc
    10501 tcttgtcccg aaccagctcg accatcggcg cattcagcca gatcggcacg cccttttcgt
    10561 cgatcgacag cttgagccgt ccgatcagcg cattgccgcc ggtcagccgc cgcgagcgct
    10621 tggtcttgaa gcgccaaggc aggtcgaaca tatatttgag catcagcttg aacagcaaag
    10681 gcttggcgcc ggggctcatc gtcagcagca gcttggcttc agccatcgtc caggtgaagc
    10741 ggccgaaggt cagcgtcagc gtgtgcggcg gctccatccg ctcgaaatcg gcgcccagtc
    10801 tgtcggccat cagcgggatc gggtcgtgcg tccgccagcc gtcgcgcgcg cccgcgacgt
    10861 ccatatgata atcggaatag gcgtaagcgg tgtaacgaac ctgcgacctc tcctcgagat
    10921 agcttagcat ccggtgcgca ttgtcgacat aggccctaag cttggtttcg ggcacatcgt
    10981 cgccgatcag cgccttgata tagccgagcg cctcctccgg gtcgtcggca tggcccttgg
    11041 cgcgcgcgtc gtcgctgttc ggaatccaga tgccgccgcc cgaggtcgcc gaagtcccgc
    11101 cataacgtgc gcttttctct accaccagca cctcggcgcc cgcgtcggcg gcggtgaagg
    11161 ccgcggtcaa agcgcccgcg cccgatccga tcaccaatat gtcgacttcg tgaacggcca
    11221 cccctgttgc tcctgcctcg aataactcgc caatttcggg ttatagaccg ttctacataa
    11281 tgattgcgtt tttgaaagga gaaatcgtta cgcaaactgt tatatattcg gcattggaat
    11341 cgatgaatgt tgacagtggc gtagcgatgc ggtctgtgga ggcatgtcga tgacgccggg
    11401 gcgccatcgt accgcgcgct ggtcaagctg gggagaagag tcgttgcgca ccgcaaccaa
    11461 ggccgggaaa aggacgcgcg gcaaggccgc gaacgcgagc cagggcgacg gcatgctcgc
    11521 gctcgacgat gtcgacgagc agatcatcgc cgcgctccgc aagaacggcc ggatcgcgaa
    11581 ccgcgacatc gcgcgcgaaa tggggcttaa cgaagcgacc gtgcgcacgc gcctgcgccg
    11641 gctggaggat gcccggatgg tgcgcgtcgt cgcgatgcgc gacctcgccg cgatgggttt
    11701 cggctatctg gcgcccgtcg gggtgcaggt gaaggggcgg cccgcgggag acgtcggcgc
    11761 cgaaatcgcc gaactcgagc gggtgatcac cgtcaacgtc gcgatcggaa cgcatgacct
    11821 cgaaatccag gtcgtggccg aaacgctgga ggagatgcag catctcctga ccggcgtgat
    11881 cgccaagatc gacggggtcg agcggatatt ccccagcctc gcgctcaagg ttttcaaata
    11941 taatccggaa tgggccccgc tatgagcacg aggatcagcc tcgacgacct cgatcacaag
    12001 atcatcgaac gcctcggcca tgacgcgcgg gtttcgaacc gcgagatcgg ccgcgaattc
    12061 gacctcaccg aaggcacgat ccgctcgcgg ctcaaacgcc tcctcgacaa tcgcgtgatc
    12121 cgcgtcgccg cggtgaccaa cgccaatcgg ctccgcaacc cgatcctcgc ctatctgtgg
    12181 gtcgaggcgg acagcgccgc ggatatcgag agagtggccg aaagcctcgc gaaattgccc
    12241 gagatcggtt ttgtcgccac gacgctcggc cgcgccgacg tgctggcgat gacattggtc
    12301 gaaaacggca atgaactcac cgacttcctc caccagacga tcgacaagat ccccggcgtc
    12361 cgccgcgtcc gctacagcct cgggcagaat ttcatcaagc acgactatcg ctggtgcgcg
    12421 ctggtcgatt gaatgatctt gtgatctgag aaccgcgtcc ttcccccttg atgggggaag
    12481 gatacgcagc cttgtccgcg tagcggctag gcgaagttgg atgggggtga tcgagcgtca
    12541 ttgcactgtc atttcagacc gaaaccgcac ccccaccgct gcgactgcga acaagttcgc
    12601 aagtctcgct gcctccccca tcaaggggga gggaagggtg ggggtcacca agttccggcg
    12661 gccgctattc cgccacccac tccgccttga tcccgtccgc ggcaagcacc ccgtcgacga
    12721 tcgcgcgggg ttccgagcgc gtgtagcaga tccgccaccc gtcgggcgtg cggcgatagc
    12781 ggtcgtgata gaagttcgcg agcagccgga agctcccggc tcgcggatca taggtcgcat
    12841 agcccagatt ccagcgcgcg ctcgcttcgt cgcccgtcac ctcgatctcg gcattatgcc
    12901 cctgatgcac gtcgacgatc ggcgtcgtcg ccgccatctg cgtgaagacg tcgatcagcc
    12961 cgtcgaggcc gaattcgccg attggcggga aatcgatcac cgcatcttcg gtgaagcagg
    13021 cgcggatcgc gccgacatcc ttggcgtcgc aggcattgag gtagcgcgcc ttcaggcggc
    13081 ggatgtcgct ttcggcttcg agccgcgcga tacgggattc aagctcggtc atcgatcggg
    13141 attatcctcg tttcggggat gggatggtcg gtggcgccga tccagcgcag cagcatcgtg
    13201 ccctgatggt gtcccgcggt atcgatccag ttgccgaagc cgggatcgcg cggcgcgacg
    13261 accagggtga gcgtgccgtc gtcgttcagc cgcgcgctgt gcccgttcac atggatgcgg
    13321 tgatggcgat aatcgagcga ttccatccag acattgtcga gctggaaatt ccagaagctg
    13381 catgcgggaa tgggtgtgtc gatctgcaac gcttcgccgg gttcgagctg ccaccagccg
    13441 tggagataga agatcgtcgg gtcgccgccg gcgcggaaga agcgcgtctg gtcgaccgtg
    13501 gcgagccggt tcatctgctc ggcgcggaaa tcctcggccc attggacgaa ggttcgcgcg
    13561 gtgcccgcga cgaaacccgt gacgcgttcg agcgcggctt cgagcccgtc cggcgacagc
    13621 ggcgccgggt aggtggggcc gtcgagcgcc tcgatcgtca ccgatgcggg aatttcgttc
    13681 gggcggtcga ggaaggtctg gcggacgagt accatcgagc tgtctttggc gagcggcagc
    13741 cagttgccct cggcgcgctc gcggctcaca atgatctcga acgatccgtc gggcgcgatc
    13801 gccatgtcgg ccgaatcgag ttcgccggtc gaggccatcg tcccgtcgat cgcatagcgg
    13861 ttcgccttgg tcccgaaact caggatcggg acgctgccgc gttggccccg gatgcgatag
    13921 cgccgcgccc ccgacaccgt cgcgttcata tagatattgt cgggattgtc ggcgccgatc
    13981 ttcgccgttt cgtgcgacgc ggcgtaaaag ctcgggaagt cgggatcggc atgttcaagc
    14041 tgcatgtcga gcgcgatgcg cagcagccgg gtgaggtagc gatagccctc ggcgcggtcg
    14101 agcgggttgg cggcggcttc ggcgctggtg atgatctcgc ctgcggcctc cagcctacgg
    14161 cagaaatcgc gccagacatc gggcgacagg atggagccct ccgtcatcgt catggcgcca
    14221 tatactggcc gccgttgacg tcgagcgtcg cgccggtgac catttttgca taatcggaga
    14281 ggaagaagag cacgctcttc gcgcaatcct cctcgggcgg aatgacgccg agcgggatg
//
LOCUS       DATMSU010000104         7456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172650,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000104 DATMSU010000000
VERSION     DATMSU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172650"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VN034_02785"
     CDS             <1..178
                     /locus_tag="VN034_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate transporter"
                     /protein_id="HWV59553.1"
                     /translation="LAGSGAPLPGASSKLEGGLAEPIGAGSTQGRLTLGSDRFFDGRA
                     FDPDDIEDYLAELP"
     gene            426..1658
                     /locus_tag="VN034_02790"
     CDS             426..1658
                     /locus_tag="VN034_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HWV59554.1"
                     /translation="MTTAIPGASAPKSSFWASGHWPTLVAAFLYFDLAFMVWVILGPL
                     APAISEDLGLTPAQKGLMVAVPTLAGAVLRVVNGLLVDRIGPKRSGAISQLIVIAGLF
                     AAWSMGVHSFAGTLALGVILGFAGASFAIALPLASRWYPPEHQGKAMGLAGMGNSGTV
                     LAALFAPVLAKLFGWNAVLGLACIPLTIVFFVYLVMAKDAPGAPAPKKLIDYFEPLKS
                     ADAWWLMAFYSVTFGGFVGLAASLPIYFTDQFHLTPVVAGYCTAACVFAGSLVRPMGG
                     ALADRIGGVKALMIVFVVAALALAGVPQAANLPAALTLFVIAMLALGTGNGSVFQLVP
                     QRFSAEIGVMTGLVGMAGGVGGFYLASSLGFAKQWSGSFGPGFYIFAGLTVVALVGLL
                     FVKGQWRATWGAAEGVRI"
     gene            1662..2864
                     /locus_tag="VN034_02795"
     CDS             1662..2864
                     /locus_tag="VN034_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWV59555.1"
                     /translation="MPRTLFILLALAPAGAAHAQSITLKPLGEARLRYESVDQDGLAA
                     NADALTLRLRAGVEAKTGRWSALVEGQGNLAITGDYFDGLHGAATRPLVADPQNIALA
                     RAQLHYASPAFAVTAGRQRIALDDERFVGAVPFRQNGQSFDAVRAEVTPVKGVKADLT
                     YAWSVRTIWGIDGVGARQQAVSGDNVFGNLSVATPAGKLAAFAYLVDQDEAATQGFRM
                     SSQSYGVRLDGNRPVGKAKIAYQLSYARQSDWHRNPKDYSAEYWLADVAVDFGGPRVG
                     VGYEILGADQGAALTSFQTPLATGFKFQGWADKFLITPPDGMRDLYASAGWGWKAVGP
                     LKAVTLQAVYHDYRSDRASRAYGDEIDLLASAKLGKCTASARYANYDADGFATDTHKF
                     WLQLDWTL"
     gene            2864..5341
                     /gene="nirB"
                     /locus_tag="VN034_02800"
     CDS             2864..5341
                     /gene="nirB"
                     /locus_tag="VN034_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HWV59556.1"
                     /translation="MEHRPLRPEADTREHLVVIGNGMAGCRAVEELLARDPARYRVTI
                     FGAEPRVNYNRIMLSPVLAGEKCFDDIVINDAAWYADNGIALVAGDPVLAIDRAAKTV
                     TTRSGLSESYDRLLIATGSDPFIIPVPGKDLSGVIAFRDMDDVDTMLAAADAGGDAVV
                     IGGGLLGLEAAHGLSLRGMKVTVIHLMPTLMERQLDEAAGWLLKNALEARGQTILTGA
                     DTAQIVGDGKVDGVRLKDGTLIPASLVVMAVGIRPSVALARDAGLAVGRGIQVDDHMV
                     TSDPAVLAVGECVEHDGQVYGLVAPLWDMCRSLADGLVEKPTGYRGSVTSTKLKVSGI
                     DVFSAGDFSGGDGCEDIVLRDASRGVYKRVIVKDDRIVGAVLYGDTADGSWYFDLLKK
                     QEDVSGVRDLLIFGQAFASGGGAADPKAAVAALSDDAEICGCNGVTKGQVVSCIAKGA
                     HSLDAVRGTCKASASCGSCTGLVENLLALTLGDDVQSGPKTMCKCTSFGHDDVRREIV
                     AQAMRSIPEVMQKLHWTTPDGCSSCRPALNYYLLCALPGDYVDDQQSRFVNERLHANI
                     QKDGTYSVVPRMWGGLTNPTELRAIADVVEKYNAPMVKVTGGQRLDIFGIKKEDLPAV
                     WADLGAAGMVSGHAYGKALRTVKTCVGSEWCRFGTQDSTGLGVKLERMSWGSWMPHKF
                     KIAVSGCPRNCAEATIKDFGIVCVDSGYELHVGGNGGIHVRATDLLCKVATEQEAMDH
                     CAAFIQLYREEARYLERTAPWIERVGLAHIVARIVDDEPGREALRARFLYSQSFSQDD
                     PWAQRAEGAEAEHHAPMARFTPQEAFA"
     gene            5338..5679
                     /gene="nirD"
                     /locus_tag="VN034_02805"
     CDS             5338..5679
                     /gene="nirD"
                     /locus_tag="VN034_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HWV59557.1"
                     /translation="MTTAEWLDIGWVDQIPVRGSRTVQVEGGDDIAVFRTAEGKVFAL
                     LDRCPHKHGRLSQGIVHGGAVACPLHNWRISLSTGEALGEDKGCTPTVPVKVDGGRVL
                     ICRASTLKAAA"
     gene            5679..>7456
                     /locus_tag="VN034_02810"
     CDS             5679..>7456
                     /locus_tag="VN034_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV59558.1"
                     /translation="MAEAIRTTCAYCGVGCGIRATVTGERTVEIAGDPDHPANRGRLC
                     SKGTHLGETVGLEGRLLTPMIGAKRASWDKALDLVAKRFKETVARHGPGSVAFYVSGQ
                     LLTEDYYVANKLMKGLIGTANIDTNSRLCMSSAVAGHTRSFGEDVVPATYDDLDAADL
                     IVLVGSNTAWCHPIVYQRIRARCEAGAKLVVIDPRRTETAEEADLHLAIRPGSDVALM
                     TGLLQHCREAGIVDETYLAAHVAVPEDFWDQLGEGNDLWSVARTCDVPAADLRHFYEL
                     FAAHPRTVTLFSQGINQSTAGTDQVNAILNLHLATARIGKPGAAPFSITGQPNAMGGR
                     EVGGLASTLAAHMDFAPQNRARVQRFWASPTIAEKPGLKAVDLFRSLGEGRIKALWVM
                     ATNPAVSMPDGNAVREALATCPFVVVSDVIEKTDTGAFAHVRLPAAAWGEKDGTVTNS
                     NRTISRQRALFPLPGEAMPDWWIVKEIGRRMGWKTAFAYDRPADIWREHCRLSTYDND
                     GARLFALPGAAQGGTAAYDAMMPFRWGGDHVFADGVYPTGDGRARLVPVAQKPLPDPL
                     AKWPLTLNTGRYRDQWHTMTRTGLAP"
BASE COUNT         1203 a         2538 c         2458 g         1257 t
ORIGIN      
        1 gctcgcaggg tcgggggcgc cgcttccggg tgcgagctcg aagctcgaag gcggactcgc
       61 cgagcccatc ggcgcaggct cgacgcaggg gcgcctcacc ctcggcagcg accgcttttt
      121 cgacggccgc gccttcgatc ccgacgatat cgaggactat ctcgctgaac tgccgtagct
      181 ttaattttat gctgcacctg cgaaataacc gcttgcggcg tgcctaaaaa tcaggcagtt
      241 taatcgcact cggcagagct gccggggcag gataaagcgg cgacggctcc aacgaggggg
      301 ctggcccgcg cggcacatgg ggagcctttc cccataatcc aacaggcaat gaagccgtca
      361 ggatgcgccc gcgtggccgc tgtcctggcg gcttttttat tgcccgtcac acaaggggac
      421 ggacgatgac gactgcaatc ccgggggctt cggcacccaa atcgagcttc tgggcaagcg
      481 gccactggcc gacgctggtc gccgcctttc tctatttcga cctcgccttc atggtgtggg
      541 tgatcctcgg gccgctcgcg cccgcgattt ccgaagatct cggcctcacc cccgcgcaaa
      601 aggggctgat ggtcgcggta ccgaccctgg ccggcgcggt gctgcgcgtc gtcaacggcc
      661 tgctcgtcga ccgcatcggg ccgaagcggt cgggcgcgat cagccagctg atcgttatcg
      721 cgggcctgtt cgccgcctgg tccatgggcg tgcacagctt cgccggcacg ctcgcgctcg
      781 gcgtgatcct cggttttgcc ggcgccagct tcgcgatcgc cctgccgctc gcgagccgct
      841 ggtacccgcc cgagcatcag ggcaaggcga tggggctcgc cggcatgggt aattcgggca
      901 ccgtgctcgc cgcgctcttc gcccccgtcc tcgcgaagct gttcggctgg aacgcggtgc
      961 tgggcctcgc ctgcatcccg ctgacgatcg tcttcttcgt ctatctcgtc atggcgaagg
     1021 acgcgccggg cgcgccggca cccaagaagc tgatcgacta tttcgaaccg ctgaagagcg
     1081 ccgacgcctg gtggctgatg gccttttatt cagtgacctt tggcggcttc gtcgggctcg
     1141 ccgcatcgct gccgatctat ttcaccgacc agttccatct caccccggtc gtcgccggct
     1201 attgcaccgc ggcctgcgtg ttcgccgggt cgctggttcg cccgatgggc ggcgcgctcg
     1261 ccgaccggat cggcggggtc aaggcgctga tgatagtctt cgtcgtcgcc gcgctggcgc
     1321 ttgccggggt gccgcaggcc gcgaacctgc ccgccgcgct cacgctcttc gtcatcgcga
     1381 tgcttgcgct cggcaccggc aatggctcgg tcttccagct cgtgccgcag cgcttctcgg
     1441 ccgagatcgg cgtgatgacc gggctcgtcg gcatggcggg cggcgtcggc ggcttctacc
     1501 tcgcctcctc cttgggcttc gccaagcagt ggagcggcag cttcggtccc ggcttctaca
     1561 tcttcgcggg gctcacggtc gtcgcgctgg tcgggctgct cttcgtcaag ggccagtggc
     1621 gcgcgacctg gggcgcggcc gaaggcgtgc ggatctaaat catgccccgc acccttttca
     1681 tcctgctggc gctcgcgccg gccggggccg cacacgccca gtccatcacc ctcaagccgc
     1741 ttggcgaagc ccgcctccgt tacgagagtg tcgatcagga cgggctcgcc gccaacgccg
     1801 acgccctgac cctgcgcctt cgcgccgggg tcgaggcgaa gaccggacgc tggtcggcgc
     1861 tcgtcgaagg ccagggcaat ctcgcgatca ccggcgatta tttcgacggg ctccacggcg
     1921 cggcgacgcg gccgctggtg gcggacccgc agaatatcgc cctcgcccgc gcccagctcc
     1981 actacgcctc gccggccttc gcggtcacgg cgggacgcca gcggatcgcg ctcgacgacg
     2041 agcgtttcgt cggtgcggtg cccttccgcc agaacggcca gagcttcgac gcggtgcgcg
     2101 ccgaggtgac gcctgtgaag ggcgtgaagg cagatctcac ctatgcgtgg agcgtgcgca
     2161 ctatctgggg catcgatggc gtcggggcgc ggcagcaggc ggtgtcgggc gacaatgtct
     2221 tcggcaatct cagcgtagcg acccccgcgg gcaagctcgc cgccttcgcc tatctcgtcg
     2281 atcaggacga agcggcgacg cagggcttcc gcatgtcgag ccagagctat ggcgtccgcc
     2341 tcgacggcaa ccggccggtt ggcaaggcga agatcgcgta tcagctgagc tatgcgcgcc
     2401 agtccgactg gcaccgcaat cccaaagact attccgccga atattggctc gccgacgtgg
     2461 cggtcgattt cggaggcccc cgagtcggcg tcgggtatga aatactcggc gccgaccaag
     2521 gggccgcgct gaccagcttt cagacgccgc tcgcgaccgg gttcaagttc cagggttggg
     2581 cggacaagtt cctgatcacc ccgcccgatg gcatgcgcga cctctatgca agcgcgggtt
     2641 ggggctggaa agccgtcggg cccctcaagg ccgtaacctt gcaagcggtc tatcacgact
     2701 atcgcagcga ccgcgccagc cgcgcctatg gcgacgagat cgacctgctg gcgagcgcga
     2761 agctcggcaa atgcaccgcc tcggcgcgct acgccaatta cgacgccgac ggcttcgcga
     2821 ccgacaccca taaattctgg ctgcaactgg actggacgct ctgatggaac atcgcccctt
     2881 gaggcccgaa gccgacacgc gcgaacatct cgtcgtgatc ggcaacggca tggccgggtg
     2941 ccgcgcggtc gaggaactcc tcgcacgcga tccggcacgc taccgcgtga cgatcttcgg
     3001 cgccgagccg cgggtcaact acaaccgcat catgctttcc cccgttctcg cgggcgagaa
     3061 atgcttcgac gacatcgtga tcaacgatgc cgcctggtat gccgacaatg gcatcgcgct
     3121 cgtcgctggc gacccggtgc ttgcgatcga ccgcgctgcc aaaacggtga cgacgcgcag
     3181 cgggctcagc gaaagctatg accggctgct gatcgcaacc gggtcggatc ctttcatcat
     3241 tccggtgccg ggcaaagacc tgtccggcgt catcgccttt cgcgacatgg acgatgtcga
     3301 caccatgctc gccgccgccg atgcaggcgg cgatgcggtg gtgatcgggg gaggcctgct
     3361 cggactggag gccgcgcacg gcctgtcgct gcgcgggatg aaggtcacgg tcatccacct
     3421 gatgccgacg ctgatggagc gccagctcga cgaagccgca ggctggctgc tcaagaacgc
     3481 actcgaagcc cgcggccaga cgatcctgac cggcgcagat accgcccaga tcgtcggtga
     3541 cggcaaggtc gatggcgtga ggctgaaaga cgggacattg atccccgcca gccttgtcgt
     3601 catggcggtc ggcattcgtc cctcggtcgc cctcgcccgc gacgccggcc ttgcggtcgg
     3661 gcgtggcatc caggtcgacg accatatggt gacgagcgat cctgctgtcc tcgcggtcgg
     3721 cgaatgcgtc gagcatgatg ggcaggttta cggcctcgtc gcgcctttgt gggatatgtg
     3781 ccgcagcctt gccgacgggc tggtcgaaaa gccgaccggc tatcgcggat cggtgacctc
     3841 gactaagctc aaggtgtcgg ggatcgacgt cttctcggcg ggcgattttt cgggcggcga
     3901 cgggtgcgag gacatcgtgc tgcgcgacgc gagccgaggc gtctacaagc gcgtaatcgt
     3961 caaggacgac cgtatcgtcg gcgcggtgct ctatggcgat acggccgatg gcagctggta
     4021 tttcgatctc ttgaagaaac aggaggatgt ctccggcgtt cgcgatctgc tgatcttcgg
     4081 ccaggccttc gcctcgggag ggggcgcggc ggaccctaag gcggccgttg cggcgctttc
     4141 cgacgatgcg gaaatctgcg gctgcaacgg cgtcaccaag ggtcaggtcg tctcgtgcat
     4201 cgccaagggt gcgcatagcc tcgacgcggt gcgcggcacc tgcaaggcgt cggcaagctg
     4261 cggttcgtgc accggcctcg tcgaaaatct gctcgcgctg acgctcggcg acgatgtcca
     4321 gtcgggcccg aaaacgatgt gcaaatgcac cagcttcggt catgacgacg tccgccgcga
     4381 aatcgtcgcg caggcgatgc gttcgatccc cgaggtcatg cagaaattgc actggacgac
     4441 gcccgacggt tgctcgtcgt gccgccccgc gctcaattat tatctgctct gcgcgctccc
     4501 gggcgactat gtcgacgacc agcagagccg cttcgtcaac gagcggctgc acgccaatat
     4561 tcagaaggac ggcacttact cggtcgtccc gcgcatgtgg ggcgggctca ccaacccgac
     4621 cgagctgcgc gcgatcgccg acgtcgtcga gaagtacaac gcgccgatgg tcaaggtcac
     4681 cggcggccag cggctcgaca tcttcgggat caagaaggaa gacctgcccg ccgtgtgggc
     4741 cgacctcggc gcggcgggca tggtctcggg ccacgcctat ggcaaggcgc tgcgcacggt
     4801 aaagacctgc gtcgggtccg aatggtgccg cttcggcacg caggattcga ccgggctcgg
     4861 tgtcaagctc gaacggatga gctggggcag ttggatgccg cacaagttca agatcgcggt
     4921 gtcgggatgc ccgcgcaact gcgccgaagc gacgatcaag gacttcggaa tcgtctgcgt
     4981 cgacagcggc tacgaactcc atgttggcgg caatggcggc atccacgtgc gcgccaccga
     5041 cctgctctgc aaggtcgcga ccgagcagga ggcgatggac cattgcgccg ccttcatcca
     5101 gctctaccgc gaggaagcgc gctatctcga gcgcaccgcg ccgtggatcg agcgcgtcgg
     5161 gctcgcgcat atcgtcgcgc ggatcgtcga tgacgagccg ggccgcgagg cgctgcgcgc
     5221 gcgcttcctt tattcgcaga gcttctcgca ggacgatccc tgggcgcagc gtgccgaagg
     5281 cgccgaggcc gaacatcatg cgccgatggc gcgcttcacc ccgcaggagg ctttcgcatg
     5341 accaccgccg aatggcttga tattggctgg gtcgaccaga tcccggttcg cggtagccgc
     5401 accgtgcagg tcgagggtgg cgacgatatc gcggtcttcc gaaccgccga aggcaaggtt
     5461 ttcgcgctac tcgaccgctg cccgcacaag catggacgac tgtcgcaggg catcgtccat
     5521 ggcggcgcgg tcgcctgccc gctgcacaat tggcggatca gtctgtcgac cggcgaagcg
     5581 cttggcgaag acaagggctg caccccgacc gtgcccgtga aggtcgacgg cggccgcgtc
     5641 ctgatctgtc gcgcaagcac gttgaaagcg gcggcctgat ggcggaggcg atccgcacca
     5701 cctgcgccta ttgcggcgtc ggctgcggca tccgcgcgac ggtgacgggt gagcggacgg
     5761 tcgagatcgc gggcgacccc gatcaccccg ccaaccgcgg tcgcctctgc tcgaagggca
     5821 cgcatctggg cgagaccgtc gggctcgaag gccgcctcct tacgccgatg atcggcgcca
     5881 agcgcgcgag ctgggacaag gcgctcgacc tcgtcgcgaa gcgcttcaag gagacggtcg
     5941 cgcgccacgg cccgggcagc gtcgccttct acgtctcggg gcaattgctc actgaggatt
     6001 attatgtcgc gaacaagctg atgaagggct taatcggcac cgcgaatatc gacaccaatt
     6061 caaggctttg catgtcgagc gcggtcgccg gacacacgcg ctcctttggc gaggatgtcg
     6121 tccccgcgac ctatgacgat ctcgacgccg ccgacctgat cgtgctcgtc ggatcgaaca
     6181 ccgcctggtg ccacccgatc gtctaccagc gcatccgcgc gcgctgcgag gcgggcgcca
     6241 agctcgtcgt catcgacccg cgccgcaccg aaaccgccga ggaagccgat ctccacctag
     6301 cgatccgccc cggcagcgac gtcgcgctga tgaccgggct gctccagcat tgccgcgaag
     6361 ctggcatcgt cgacgaaacc tatctcgccg cgcatgtcgc ggtgccggaa gatttctggg
     6421 accagcttgg cgaggggaat gacctgtggt cggtggcgcg cacttgcgat gtccccgccg
     6481 ccgacctcag gcatttctac gagctgttcg ccgcccaccc gcgcaccgtc actttgttca
     6541 gccaggggat caaccagtcg accgcgggga ccgatcaggt caacgcgatc ctcaacctcc
     6601 acctcgcgac cgcgcgcatc ggcaagcccg gcgccgcgcc cttttcgatc accggccagc
     6661 ccaatgcgat gggcgggcgc gaggtcggcg ggctcgcctc gaccctcgcc gcacatatgg
     6721 atttcgcccc ccaaaaccgc gcgcgcgtcc agcgcttctg ggcgtcgccg acgatagccg
     6781 aaaagcccgg cctgaaggcc gtcgacctgt tccgcagcct cggcgagggc cgcatcaagg
     6841 ccctgtgggt gatggcgacc aaccccgcgg tgtcgatgcc cgacggcaat gcggtacgcg
     6901 aggcgctcgc gacctgcccc ttcgtcgtcg tgagcgacgt gatcgaaaag accgacaccg
     6961 gcgccttcgc gcatgtccgc ctgcccgccg cggcgtgggg cgagaaagac ggtaccgtca
     7021 ccaacagcaa ccgcaccatc agccgccagc gcgcgctctt cccgctcccc ggcgaagcga
     7081 tgcccgattg gtggatcgtc aaggaaatcg ggcggcggat ggggtggaag accgccttcg
     7141 cctacgaccg tcccgccgac atctggcgcg agcattgccg cctgtcgacc tatgacaatg
     7201 atggcgcgcg ccttttcgcg ctgcccggtg cggcgcaggg cggcaccgcc gcctatgacg
     7261 cgatgatgcc ctttcgctgg ggcggcgatc atgtctttgc cgatggcgtc tacccgaccg
     7321 gcgacggacg cgcacggctg gtgccggtgg cgcaaaagcc gctgcccgac cccctcgcca
     7381 aatggccgct gacgctcaac accggccgct accgcgacca gtggcacacg atgacgcgca
     7441 ccggcctcgc gccgaa
//
LOCUS       DATMSU010000105         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_11488, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000105 DATMSU010000000
VERSION     DATMSU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11488"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VN034_02815"
     CDS             complement(<1..919)
                     /locus_tag="VN034_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWV59559.1"
                     /translation="MPQPQDDHRASRKPGQRRGRLIGGAVLIALSLASCGLFGERGPV
                     KVAAIGTLNLAATPLSGELSAANAALLDATSQGLVSYDGEGQIDTGLADRWTVTTDGR
                     SYIFRLREAKWTNGRKVTAEDVAEILRSYLAPASRHVLKDDFPEVETIKAMTDTVIEI
                     RLTVPQPAILELLAQPSMAIVNKGMGWGPMRARKIGRAVLLSPVPDPLAEDPEAAEAA
                     ANDPAASIELVGTSPEGALARFKNGYADGVIGGRFSTLPYFVASNIGRSRLVVDPVPG
                     LFGLSFVRAEGFLATDVNRDALVRAVRRER"
     gene            complement(1041..>1764)
                     /locus_tag="VN034_02820"
     CDS             complement(1041..>1764)
                     /locus_tag="VN034_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59560.1"
                     /translation="AGARGTRFGFQGPSQGGWIAPLAATRTQVDFLIVSFGLAVSVAD
                     EDREAIAFQMGLKGYGPDVIAKAQEIGAATTRIFASGMTDGIEELDAVRARYRDEPWY
                     KDVYGNYTHLILGMTAEEIRTKGQVYRFGTPVHYDPMPTLRAVSVPQLWALGGQDIDA
                     PAGETARRIKELIAAGQPITLAVFPKAEHGMTEFETAPDGSRVSTRFTPGYFRLLIDY
                     AKGAPLASEYGDAEITRPAAKK"
BASE COUNT          286 a          569 c          626 g          283 t
ORIGIN      
        1 gccgctcgcg ccggaccgcg cggacgagcg cgtcgcggtt gacgtcggtc gcgaggaacc
       61 cctcggcgcg cacgaacgac aggccgaaca gcccgggcac cggatcgacg acgaggcgcg
      121 agcggccgat gttcgacgcg acgaaatagg gcagggtcga aaagcggccg ccgatcaccc
      181 catcggcata gccattcttg aaccgcgcga gcgcgccctc gggcgaggtg ccgacgagtt
      241 cgatcgaggc cgcgggatcg ttggcggccg cctcggcggc ttcgggatcc tcggcgagcg
      301 gatcggggac gggcgagaga agcaccgcgc ggccgatctt gcgcgcgcgc atcggacccc
      361 agcccatgcc cttgttgacg atcgccatcg acggctgggc aagcagttcg aggatcgcgg
      421 gttgcggcac cgtgaggcgg atttcgatga cggtgtcggt catcgccttg atcgtctcga
      481 cctccggaaa atcatccttg aggacgtggc ggctggcggg cgcgagatag gagcgcagga
      541 tctcggcgac atcctcggcc gtcaccttgc gcccgttggt ccatttggct tcgcggaggc
      601 gaaaaatata gctgcgcccg tcggtggtga cggtccagcg atcggcgaga ccggtgtcga
      661 tctgaccctc gccgtcatag ctgacgagcc cctgcgaggt cgcatcgagc agcgcggcat
      721 tggccgccga caattcgccg gaaagcggcg tcgcggcaag gttgagcgtg ccgatcgccg
      781 cgaccttcac cggaccgcgc tcaccgaaca ggccgcaact ggcgagcgac aaggcgatca
      841 ggacggcgcc gccgataagg cggccgcggc gctgccctgg ctttctgctg gcccggtgat
      901 cgtcctgagg ttgcggcatg ggaggcatca taaggataag cggcaaaacc ggaaggggcg
      961 attgggagcg tctgccgcgg gaaggcgcga acggctgatc gggcggcgcg ggttgcgggc
     1021 aaattccggc ccgtcgaaga tcatttcttc gccgctgggc gcgttatttc ggcatcccca
     1081 tattcggacg cgagcggcgc gcccttggca tagtcgatca gcaaccggaa atagcccggc
     1141 gtgaagcggg tcgacacgcg cgatccgtcg ggcgcggtct cgaattcggt catgccatgt
     1201 tcggccttgg gaaagacggc gagggtgatc ggctggcctg cggcgatcaa ctccttgatc
     1261 cgccgcgcgg tttcgccggc cggcgcgtcg atatcctgcc cgccgagtgc ccagagctgc
     1321 ggcacgctga ccgcgcggag cgtcggcatc gggtcgtagt ggacgggcgt gccgaagcga
     1381 tagacctgcc ccttggtcct tatctcttcg gcggtcatgc cgaggatgag gtgggtataa
     1441 ttgccgtaga catccttgta ccacggctcg tcgcgatagc gggcgcgcac cgcgtcaagc
     1501 tcctcgatcc cgtcggtcat gccgcttgcg aagatgcggg tcgtcgcggc gccgatttcc
     1561 tgcgccttgg cgatcacgtc ggggccatag cctttgagcc ccatctggaa cgcgatggct
     1621 tcgcgatcct cgtcggcgac cgataccgcg aggccgaagc tgacgatcag gaaatcgacc
     1681 tgcgtccgcg tcgccgcgag cggggcgatc cagccgccct ggctcggccc ctgaaagccg
     1741 aagcgcgtcc cgcgcgcgcc cgcc
//
LOCUS       DATMSU010000106         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_182267,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000106 DATMSU010000000
VERSION     DATMSU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182267"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..384
                     /gene="groES"
                     /locus_tag="VN034_02825"
     CDS             70..384
                     /gene="groES"
                     /locus_tag="VN034_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWV59561.1"
                     /translation="MHFRPLHDRVVVRRIEAEEKTSGGIIIPDTAKEKPQEGEVVAVG
                     PGARAEDGTVTALDVKAGDRILFGKWSGTEVRIDGEELLIMKESDILGVIEQAEALKQ
                     AA"
     gene            430..2049
                     /gene="groL"
                     /locus_tag="VN034_02830"
     CDS             430..2049
                     /gene="groL"
                     /locus_tag="VN034_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818487.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWV59562.1"
                     /translation="MAAKEVKFASDARDRMLRGVDTLANAVKVTLGPKGRNVVIDKSF
                     GAPRITKDGVTVAKEIELADKFENMGAQMLREVASKQNDKAGDGTTTATVLAQAIVRE
                     GSKAVAAGMNPMDVKRGIDLAVTTVVEDLKAHAKTVEANSEIAQVATISANGDEEVGK
                     ILAEAMEKVGNEGVITVEEAKSLATELETVEGMQFDRGYLSPYFITNAEKLKVELDDP
                     YILIHEKKLSNLQAMLPLLESVVQSGRPLLIIAEDVEGDALATLVVNRLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAVLTGGNVVSEELGIKLESVTINMLGRAKKVVIDKDNTTIVD
                     GVGSKSDIDGRVAQIRQQIETTTSDYDREKLQERLAKLAGGVAVIRVGGATEVEVKEK
                     KDRVDDALHATRAAVEEGILPGGGIALLRSLKALEGLKAANDDQQSGIDIVRRALRAP
                     ARQIADNAGEDGAWIVGKLLESDDYSWGFNAATGEYQDLVKAGVIDPAKVVRTALQDA
                     ASVASLLITTEALVAELPKEEKAAPMPAMDF"
BASE COUNT          434 a          645 c          707 g          291 t
ORIGIN      
        1 tcatcggcgc cgcgcgtccc ggacaggcgc gccggcttat catatcgtta tgcaacatgg
       61 aggttatgca tgcatttccg tcccttgcac gaccgtgtgg tcgtccgccg catcgaagcc
      121 gaggagaaga cctcgggcgg catcatcatt cccgacaccg ccaaggaaaa gccgcaggag
      181 ggcgaagtcg tcgccgtcgg cccgggggcg cgcgccgagg acggcacggt caccgcgctc
      241 gacgttaagg ccggcgaccg gatcctgttt ggcaaatggt cgggcaccga ggtgcgcatc
      301 gacggcgagg aattgctcat catgaaggag agcgacatcc tcggcgtcat cgagcaggcc
      361 gaggcgctca agcaggcggc gtagcgccca ttcatcaccc actgaaagga cagcaagaag
      421 gaggtaaaaa tggctgccaa ggaagtgaaa tttgcgtcgg acgcgcgtga tcggatgctg
      481 cgcggcgtgg atacgcttgc gaatgcagtg aaggtcacgc tgggcccgaa gggccgcaac
      541 gtcgtgatcg acaaaagctt tggcgcaccg cgcatcacca aggacggcgt caccgtcgcc
      601 aaggaaatcg agcttgccga caagttcgaa aatatgggcg cgcagatgct gcgtgaggtc
      661 gcgagcaagc agaacgacaa ggccggcgac ggcacgacga ccgcgaccgt gctcgcgcag
      721 gcgatcgtcc gcgaaggctc aaaggcggtc gccgcgggca tgaacccgat ggacgtcaag
      781 cgcggcatcg accttgccgt caccacggtc gtcgaggacc tcaaggcgca tgccaaaacg
      841 gtcgaggcga atagcgagat cgcgcaggtc gcgaccatct cggcaaatgg cgacgaggaa
      901 gtcggcaaga tcctcgccga ggcgatggag aaggtcggca atgagggcgt gatcaccgtc
      961 gaggaagcga agagcctcgc gaccgagctc gaaacggtcg aaggcatgca gttcgaccgc
     1021 ggctacctca gcccctattt catcaccaac gccgagaagc tgaaggtcga actcgacgac
     1081 ccctatatcc tcatccacga gaagaagctc tcgaatctgc aggcgatgct gccgctgctc
     1141 gagagcgtcg tccagtcggg ccggccgctg ctgatcatcg ccgaggatgt cgagggcgac
     1201 gcgctcgcaa ccctcgtcgt caatcgcttg cgcggcgggc tcaaggtcgc cgcggtcaag
     1261 gcgccgggct ttggcgatcg ccgcaaggcg atgctcgagg atattgcggt gctcaccggc
     1321 ggcaatgtcg tcagcgagga actcggcatc aagctcgaga gcgtgacgat caacatgctc
     1381 ggacgcgcca agaaggtcgt catcgacaag gataatacga cgatcgtcga cggggtgggc
     1441 tcgaagtccg atatcgacgg ccgcgtcgcg cagatccgcc agcagatcga gacgacgacc
     1501 agcgattacg accgcgagaa gctgcaggag cgcctcgcca agctcgccgg cggcgtcgcg
     1561 gtcatccgcg tcggcggcgc gaccgaggtc gaggtcaagg agaagaagga ccgcgtcgac
     1621 gacgcgctcc acgcgacgcg cgcggccgta gaagaaggca ttcttccggg cggcggcatc
     1681 gcgctgctcc gctcgctgaa ggcgctcgag ggcctgaagg ctgcgaacga cgaccagcag
     1741 tcgggcatcg acatcgtccg ccgcgcgctc cgcgccccgg cgcggcagat cgccgacaat
     1801 gcgggcgagg acggtgcctg gatcgtcggc aagctgctcg aaagcgacga ctatagctgg
     1861 ggcttcaatg cggccaccgg cgaatatcag gacctcgtca aggccggcgt gatcgaccct
     1921 gccaaggtcg tgcgcaccgc gttgcaggac gcggcctcgg tcgcctcgct cctgatcacc
     1981 accgaagcgc tcgtcgccga gctgccgaag gaagagaagg ccgcgccgat gccggcgatg
     2041 gacttctgac gagatagggc ggcgggcgac cgccgcc
//
LOCUS       DATMSU010000107        10137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_87453, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000107 DATMSU010000000
VERSION     DATMSU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10137
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87453"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VN034_02835"
     CDS             complement(<1..842)
                     /locus_tag="VN034_02835"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWV59563.1"
                     /translation="MSDPLATVMTAMLGCRLPVIQTAMGWIAEPQLVAATAEAGGFGF
                     LGAAVMTPAEAGAKISRLRQLTDKPFGVNFHMFQPGAADLVEIILSHRDQVRAVSFGR
                     GPDAAMIGRFRDAGILCVPTVGALKHAQKMASLGVDMLVVQGAEGGGHTGAVATTVLL
                     PQVLDSVDLPVVAAGGFADGRGLAAALAYGAAGIAMGTRFLMTRESPVPEAIKARYIA
                     AGTDNIEVTRAVDGLPQRMIRNARLARLTAAGAIASWRNALAAGFEMKRATGASWGDL
                     IRAA"
     gene            complement(839..1741)
                     /locus_tag="VN034_02840"
     CDS             complement(839..1741)
                     /locus_tag="VN034_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWV59564.1"
                     /translation="MTTATPEAADIVYETDEPVLYEVKDGVAWVTMNRPRYNNAQNSQ
                     MTYALDDAFRRAVDDPAVRVIILAGTGKHFSAGHDIGTPGRDIARTFDRAHLVPDHVG
                     RPGAEMLYTREQEVYLGMCRRWRDVTKPTIAMVQGACIAGGLMLAWVCDLIVASDDAF
                     FQDPVSRLMGIPGVEYFAHAFELPPRVAKEFLLLGDRMPAGRAYDLGMVNKVVPRKEL
                     EAATAEMAAKLAAQASLGMWLTKQAVNHVEELRGKRSAMDAVFHMHHFAHAQNDLTTG
                     NSLGGVTGKSAAADNRGANEAGEG"
     gene            complement(1863..2657)
                     /locus_tag="VN034_02845"
     CDS             complement(1863..2657)
                     /locus_tag="VN034_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HWV59565.1"
                     /translation="MVENQATLADLLVAACAEVWRGDPEWLATGITPIPRLGASLAKL
                     TFNPRLMITDGENMFLEEPAPIGPREGGPTAEGWAPYARTFDNLWGGRRHALVAPVQL
                     DRFGQSNISVIGDHAAPKAALLGARGFPGNSISHPNSYFFPAHNRRALVEGEVDFVCS
                     AGYNPARWPGGKIPHRLELRRIVTNLCVMDFRGPAHAIRLVSLHPGVPIEEVRDNTGF
                     DILVEDGEIAETAVPDEAALAIIRRLDPHDIRASLIKGNPPAIRAG"
     gene            complement(2669..3538)
                     /locus_tag="VN034_02850"
     CDS             complement(2669..3538)
                     /locus_tag="VN034_02850"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWV59566.1"
                     /translation="MLDKRMPLDAIVAELRDGMTIAIGGWATRRKPMALVRAIARSSL
                     KDLTVIAYGGPDVGVLAASGKIRQLIFAFVSMDQIPIEPHFKAARQAGMQVWEMDEGM
                     LHWGLRAAAMHLPFLPTRVGIGTAIADDPRFRTVTSPYDDGETLIAMPALRPDVALLH
                     VHRSDEKGNVLTLSPDPFFDELMARAAERTFVTAEKMVSTAELGLPGNARYHLVERAL
                     ISGVAEVPFGAHPTSAAPDYQIDMAHLRTYVAAEGDAWAAYREQYVDVDPAAYVAAVG
                     GAEAIRAIPAPIY"
     gene            complement(3626..4771)
                     /locus_tag="VN034_02855"
     CDS             complement(3626..4771)
                     /locus_tag="VN034_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV59567.1"
                     /translation="MPLGNTKLISADSHFNEPGDLFTKRVAQKFKDRAPRMESFPEGD
                     GWIFEGLEGPRNFGWNACAGLPPEETKAWMRFDDMRKGGWDPAERLKEQDRDGVTAEV
                     MYPTPSIQAAIALVEDEEFHLELVRAYNDWVSEYVAYDPSRFCGLVFLPNRGGVKVAV
                     DEMNRVLGRPGMRAVMAQAYPTGTTVMTDEHDYLWAAINERDVSFNIHVALGIYKPSP
                     HKAKLPGYGRFFDAPNRCIEFIFNGVFDRYPNLKVAFAEVDFGWVPYVKEQIDNNYQR
                     LEKVNHFGLQRLPSEYIDEHFYFGYMTDSFGLNNLNYMNPERVLWSTDYPHISADYPY
                     SWRTIQASMSGVPGPVKQAILHGNAQKLYKFPEVQPVEVKGQPALTA"
     gene            complement(4881..6131)
                     /locus_tag="VN034_02860"
     CDS             complement(4881..6131)
                     /locus_tag="VN034_02860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59568.1"
                     /translation="MATMTGASEAVPAAAAPQVDDVARWRRNLVVLILLTGGVKSTLA
                     FTAVVPGLQLIAAEFHNAADSILSAQFVITLAPIGMALAGLIAGLFVRTSNLRGTMFV
                     ALGVSTIAGLMPLVIHNYTLLLATRFILGFSAVVADVAMTSVLAAYFTGGMRSKLIGF
                     RQSISHISTVITMLASGWLAQHYGWRASSLLFVIPGLLLLLAFVAFDRPISREQQEQA
                     QEGAQLSLVQLLPLLLLSLIMALGHAMPHYQMPFLLRENGITNAELVSYVPALSAGVS
                     ILSALVFGFVYARFGFATLVAAATLMGIGFIGSGLATSYEMILFFIVIEGVGAGWTMP
                     FFLTRILDRVTTAQRNHAVGLTLASLFIGQFMNPFVTKPIRDAVGLHATFVIMGVGLI
                     VITGVLGLIVIVRARAKERALAAA"
     gene            complement(6364..8649)
                     /locus_tag="VN034_02865"
     CDS             complement(6364..8649)
                     /locus_tag="VN034_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59569.1"
                     /translation="MAAALAATMPAAAFAQDVQPEPAPADTQPAMARQGGVEDIIVTA
                     RRREERAQETPVSITAISSDQLSDLNIVRIDGLTQLAPSLRITQASGSGNAPAVYIRG
                     IGTLSTALYVEPAVGVYLDGVYTPRPSGNTFDLPDIASVEVLRGPQGTLFGRNTTGGA
                     ILLATRNPEDEFGVQANFSYGSRNEITASSVVQFGQLGNSPFSFKMSGQVHSRDGWVE
                     SPGVSESKWGGALYSYGVGAALRGELGGDFIVDLRGRYNKVNSYTSWEALAGSAVGSA
                     YFQGSGTVNGPAYPIGVGPRDFSYRDPRTNGESSVESYGGVLTLEKDFSDALTVKSIT
                     GYNEINQVLRGNLGGGATRGAVANPAVPGQPIEWVTAHVTPSNPGQQKQLTQELQFLG
                     DVGDFNYLVGLYYYDEKVDETINTILNSPLSPTLAIRLDRSTSFSIASKSYAAFAQAG
                     WKPAFADGRLELVGGIRYTKDKKTLDSMSVSTTIVTTTTTQSRKDDWDNLGWLGSASY
                     KVTPDILLYARASSAYRSGGYNAPTVGAAPFDPETATSYEAGIKSDWFDRIVRLNISA
                     FQTDYKDLQVNGYNIATNTNQLTNAGSARYRGFEVEGQFVLGGLKLDGNVGHVDPVYK
                     EYILAVGGVPTNVADDAVFANVPHWTYHVGGQYTIDTASAGSFTLRGDYSGKSNSPTY
                     TLIAQAPNTAQVPLMGKESNLSARLMWEFEVNGRKLKAQVFGENLTNNRYLTFASDFG
                     AILAGTYNRPRYYGFSIGIDL"
     gene            complement(9290..9366)
                     /locus_tag="VN034_02870"
     tRNA            complement(9290..9366)
                     /locus_tag="VN034_02870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9330..9332),aa:Arg,seq:ccg)
     gene            9486..>10137
                     /locus_tag="VN034_02875"
     CDS             9486..>10137
                     /locus_tag="VN034_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HWV59570.1"
                     /translation="MKPSEQSWPMAADLYGEMQLEANPPARHRWRDYLPGILVTAIAA
                     LAAAWLADHYAAPIVLMGLLIGLALSFLSQDRRTHAGLDLMSQTALRIGIVLVGARIT
                     AGQLAELGPLPFALLVLIMLAVIVMTVATAPLFRQDKQAALLAGGATAICGASAALAL
                     YSLIGDKRIDQARFTLTLVGITVASALAMTLYPVLAAQLGLSDAQAGFLIGASVHDV"
BASE COUNT         1901 a         3295 c         3146 g         1795 t
ORIGIN      
        1 tgcgcggcgc ggatgaggtc gccccacgag gcgcccgtcg cgcgcttcat ttcgaacccc
       61 gccgcaagag cattccgcca tgaagcaatc gcgccggcgg cggtcagccg cgcgagccgc
      121 gcgttgcgga tcatccgctg cggcaggccg tcgaccgcac gcgtcacttc gatattgtcg
      181 gtgcccgcgg cgatgtagcg cgccttgatc gcctccggca ccgggctttc gcgtgtcatc
      241 aggaaccgcg tccccatcgc gatccccgcc gcaccatagg ccagcgccgc cgcgagcccg
      301 cgcccgtcgg caaaaccgcc cgccgcaacg acgggcagat cgacgctgtc gagcacctgc
      361 ggcagcagca cagtggtagc caccgccccc gtgtgccccc caccctcggc gccctgcaca
      421 acgagcatat ctacacccag cgacgccatt ttttgcgcat gcttgagcgc gccgacggtc
      481 ggcacgcaaa ggatgcccgc atcgcggaaa cgcccgatca tcgccgcgtc gggaccacgt
      541 ccgaagctga cggcacggac ctggtcgcga tgggagagga ttatctcgac caggtccgcg
      601 gcgcccggct ggaacatatg aaagttcacg ccgaacggct tgtccgtcag ttgccgcaag
      661 cggctaatct ttgcgcctgc ctcggcgggc gtcattaccg ccgcgccgag aaaaccaaaa
      721 ccgcccgcct cggccgtcgc ggcgacgagc tgcggctcgg cgatccagcc catcgcggtc
      781 tggatcaccg gcaaccggca gccgagcatc gccgtcatca ccgtggcgag cgggtcgctc
      841 atccctcgcc cgcttcattc gcgccgcgat tatccgccgc agcgctcttg cccgtcaccc
      901 cgccgagcga attgccggtg gtgagatcgt tctgggcatg cgcaaaatga tgcatgtgga
      961 agacggcgtc catcgcgctg cgcttgccgc gcaattcctc gacatgattg accgcctgct
     1021 tggtaagcca catgccgagc gaggcctgcg ccgcgagctt cgccgccatt tcggcggtcg
     1081 cagcctcgag ttccttgcgc ggcacgacct tgttgaccat accgagatca taggctcgcc
     1141 cggcgggcat acggtcgccg agcagcagaa actccttggc gacgcgcggc ggcagttcga
     1201 acgcgtgcgc gaaatattcg acccccggaa tgcccatcaa ccgcgacacc ggatcctgga
     1261 aaaaagcgtc gtccgacgcg acgatcaggt cgcaaaccca tgcgagcatc agcccaccgg
     1321 cgatgcacgc cccctgcacc atcgcgatcg tcggcttggt gacgtcgcgc cagcggcggc
     1381 acatacccag atagacttcc tgctcgcgcg tatagagcat ttcggcgccc ggacgcccga
     1441 catgatcggg caccagatgc gcgcggtcga aggtccgcgc gatgtcgcga cccggcgtcc
     1501 cgatgtcgtg tccggcactg aaatgcttgc ccgttcccgc taatatgatc accctgaccg
     1561 cgggatcgtc gaccgcacgg cggaacgcgt cgtcgagcgc ataggtcatc tggctgttct
     1621 gcgcattgtt atagcgcggg cggttcatcg tcacccacgc gacgccatcc ttgacctcat
     1681 agaggaccgg ctcgtcggtt tcgtacacga tgtccgccgc ttcgggcgtg gccgtcgtca
     1741 tggcgcaatc ctctcagtgt ttcgactatc aatcattata atgttaagag tcgtcaacag
     1801 cgggggcgat agcttcgcgg caagtcagcc gtgctttaaa gacatcacga cgccccgagc
     1861 gatcaccccg cgcgaatggc gggcggattt cccttgatca acgacgcgcg aatatcgtgc
     1921 ggatcgaggc gccggatgat cgccaatgcg gcctcgtcgg gcacggccgt ttcggcgatc
     1981 tcgccgtctt cgacgagaat gtcgaaaccg gtgttgtcgc gcacctcttc gatcgggacg
     2041 ccgggatgaa gcgagacgag gcggatcgcg tgggcgggtc cgcggaaatc catcacgcaa
     2101 agattggtga cgatccgccg cagttccagc cggtgcggta tcttgccgcc cggccagcgc
     2161 gccggattat aacccgccga acagacgaaa tcgacttcac cctcgaccag cgcgcgccgg
     2221 ttatgcgccg gaaagaaata gctgttcgga tgcgaaatgc tgttacccgg aaagccgcgc
     2281 gcgccaagca gtgccgcctt gggtgcggca tggtcgccga tcacgctgat gttcgactgg
     2341 ccgaaacggt cgagctgtac cggtgcgaca agcgcatgac ggcggccgcc ccataaatta
     2401 tcgaaggtcc gcgcataggg cgcccaccct tccgccgtgg gcccgccctc gcgcgggccg
     2461 atcggggcgg gttcctcaag aaacatattc tcgccgtcgg tgatcatcag ccgcgggttg
     2521 aaggtcagct tggcaaggct cgcgccgagc cgcgggatcg gagtgatccc ggtcgcgagc
     2581 cattcgggat caccgcgcca gacctcggca caggcggcga cgagcaagtc ggcgagcgtg
     2641 gcttgatttt cgaccacgcc gtcctccctc aatagatcgg ggcgggaatg gcgcggatcg
     2701 cctccgcccc gccaacggcc gcgacatagg cggccgggtc gacatcgaca tattgttcgc
     2761 gatacgcggc ccatgcatca ccctcggcgg cgacgtaggt gcgcaaatgc gccatatcga
     2821 tctgatagtc gggcgcagca ctggtcgggt gcgcgccgaa cggcacctcg gcgacgccgc
     2881 tgatcagcgc gcgttcgacc agatggtagc gcgcgttgcc gggcaggccc agctcggcgg
     2941 tcgaaaccat cttttccgcg gtcacgaagg tccgctcggc ggcgcgcgcc atcagttcgt
     3001 cgaagaaggg gtcgggcgac agcgtcagca cattgccctt ctcgtcgctg cgatggacgt
     3061 gaagcagcgc gacgtcgggg cgcagcgcgg gcatcgcgat cagcgtctcg ccatcgtcat
     3121 aggggcttgt aaccgtcctg aagcgcggat cgtcggcgat cgccgttccg atcccgaccc
     3181 gcgtcggcaa gaacggcaag tgcatcgcgg cggcgcgcag cccccaatgg agcatgccct
     3241 cgtccatttc ccacacttgc atgcccgcct gacgcgcggc cttgaaatgc ggttcgatcg
     3301 gaatctggtc catcgagacg aaagcgaaga tcagctggcg gatcttgccc gacgccgcaa
     3361 gcacgccgac atcggggccg ccataggcga tcaccgtcaa atccttgagc gacgatcgcg
     3421 caatcgcgcg caccagcgcc atcggcttgc ggcgcgtcgc ccagccaccg atcgcgatcg
     3481 tcattccgtc acgcagttcc gccacgatgg cgtcgagcgg catccgtttg tcgagcatct
     3541 tcattctcgc gcaggcaaag cggggttggc cgcccgtccc cgcaaggacg ggcgccgagc
     3601 cccactgcat tgtcggccta tcggctcagg cggtcagcgc cggctgaccc ttgacttcga
     3661 ccggctggac ttccgggaat ttgtagagct tctgcgcgtt gccgtgcagg atagcctgct
     3721 tcaccgggcc gggaacgccc gacatcgatg cctggatcgt gcgccagctg tacggatagt
     3781 cggcgctgat gtgcggatag tcggtcgacc acaacacgcg ctcggggttc atatagttga
     3841 ggttattgag cccgaagctg tcggtcatgt aaccgaagta gaaatgctca tcgatatatt
     3901 cgctgggcag ccgctgcaga ccgaaatgat tgaccttttc aaggcgctgg tagttgttgt
     3961 cgatctgctc cttcacatag ggaacccagc cgaagtcgac ttcggcaaag gcgaccttca
     4021 ggttcgggta gcgatcgaac acgccgttga agatgaactc gatgcagcgg ttcggcgcat
     4081 cgaagaagcg gccatagccg ggcagcttcg ccttgtgcgg gctgggcttg tagatgccga
     4141 gcgcgacgtg aatgttgaag ctgacatcgc gttcgttgat cgcggcccac agataatcat
     4201 gctcgtcggt catgacggtg gtgccggtcg gataggcctg cgccatgacc gcgcgcatac
     4261 cgggacggcc gagcacgcgg ttcatctcgt cgaccgccac cttcacgccg ccgcgattgg
     4321 ggaggaaaac cagtccgcag aaccggctgg gatcataggc gacatattcc gaaacccagt
     4381 cgttataggc gcggacgagc tcgaggtgga attcctcgtc ctcgaccagc gcgatcgccg
     4441 cctgaatcga cggggtcgga tacatgacct cggcggtcac gccgtcgcgg tcctgttcct
     4501 tcaggcgctc tgccggatcc cagccgccct tgcgcatgtc gtcgaagcgc atccatgcct
     4561 tggtttcctc gggcggcagg cccgcgcagg cgttccagcc gaaattgcgc ggcccttcga
     4621 ggccttcgaa gatccagccg tcaccctcgg ggaagctttc catgcgcggc gcacgatcct
     4681 tgaatttctg cgcgacgcgc ttggtgaaca ggtcgcccgg ttcgttgaaa tggctatccg
     4741 ccgaaatcag cttggtattc cccagaggca tcagtcttct cctacatttc tatcttataa
     4801 acatttatat gatagcgagg aaaaaggcaa agctcttttg gccccgctgg agcatttttc
     4861 gggtgaaatc ttgcgaaagg tcaggcggcg gccagcgccc gctccttggc cctcgcccgc
     4921 acgatgacga tcaggcccaa gacgccggtg atgacgatca gcccgacgcc catgatgaca
     4981 aaagtggcgt gcaatcccac cgcatcgcga atcggcttgg tcacgaaggg gttcatgaac
     5041 tggccgatga acagcgacgc gagcgtcagt ccgactgcgt gattgcgctg cgccgtcgtc
     5101 acccggtcga ggatgcgggt caggaagaac ggcatcgtcc agcccgcgcc gacaccctcg
     5161 ataacgatga agaacaggat catctcatag ctggtagcca gccccgaccc gatgaaaccg
     5221 atgcccatca gggtcgccgc ggcgaccaat gtcgcaaagc cgaagcgcgc gtaaacgaag
     5281 ccgaacacca gcgccgacag gatcgacacc cccgcgctga gcgccggcac gtacgaaacg
     5341 agttcggcgt tggtgatccc gttttccctg agcaggaacg gcatctggta atgcggcatc
     5401 gcgtggccga gcgccatgat caacgacagc agcagcagcg ggagcagttg caccagcgac
     5461 agctgcgcgc cctcctgtgc ctgttcctgc tgctccctcg agatcgggcg atcgaaggcg
     5521 acgaaggcca gcagcaacaa caggcccgga atgacgaaga gcagcgacga cgcgcgccag
     5581 ccataatgct gcgccagcca gcccgacgcg agcatcgtga tcacggtgct gatgtgcgaa
     5641 atcgactggc ggaacccgat cagcttcgac cgcatgccgc ccgtgaaata ggcggcgagc
     5701 acgctggtca tcgcgacatc tgcaaccacc gcgctaaacc cgaggatgaa gcgggtggcg
     5761 agcagcagcg tgtaattgtg gatcaccagc ggcatcaacc cggcgatcgt cgatacgccg
     5821 agcgcgacga acatcgtgcc gcgcaaattg ctcgtgcgga cgaacaggcc cgcgatcagc
     5881 ccggcgagcg ccatgccgat cggcgcgagc gtgatcacga actgcgcgct caggatcgaa
     5941 tcggcggcgt tatggaattc ggcggcaatc agctgcaggc cgggaaccac tgccgtgaag
     6001 gcgagcgtcg acttcacccc gccggtcagg aggatcagga caacgagatt gcggcgccag
     6061 cgcgcgacat cgtcgacctg cggcgccgct gccgccggca cagcttcact tgctccggtc
     6121 atcgtggcca tctcccgaat atcctctctc gcctgttgtt atagaggcta gcacccgccg
     6181 cgcagctttg ccgttcgcga tcgggcaccg ccctgttgcc ggacgcgcat cgttgcagcg
     6241 caccgacgga cataagcctt tatcgacgga cgatgtccat catactacaa aagatagcta
     6301 ggaaagagtc gtcaccgacg acggcgcggc cggagcaggc cgtcgtcgat gcgcgttgcc
     6361 gctttagaga tcgatgccga tcgagaaacc gtaatagcgc ggacgattat aggtacccgc
     6421 gaggatcgcg ccgaaatccg acgcgaaggt caggtagcgg ttgttggtca gattctcgcc
     6481 gaaaacctgc gccttgagct tgcggccgtt gacctcgaat tcccacatca ggcgcgcgct
     6541 gagattgctc tccttgccca tcagggggac ctgcgcggta ttcggcgcct gcgcgatcag
     6601 cgtataggtc ggcgaattgc tcttgccgct atagtcgccg cgcagcgtga aactgcccgc
     6661 cgaggcggtg tcgatcgtat attggccgcc gacatgatag gtccagtgcg ggacgttcgc
     6721 gaagaccgca tcgtcggcga cattagtggg aactccgccg accgcgagga tatattcctt
     6781 atacaccggg tcgacatggc cgacattgcc gtcgagcttg aggccaccaa gcacgaactg
     6841 cccttcgacc tcgaagccgc gatagcgcgc gctgccggca ttggtgagct ggttggtatt
     6901 cgttgcgata ttatagccgt tgacctgtag gtccttgtag tcggtctgga acgcggaaat
     6961 gttcaatcgc actatccggt cgaaccagtc gctcttgata ccggcttcat agctggtcgc
     7021 ggtctccgga tcgaacggcg ccgcaccgac ggtcggggcg ttgtagccgc ccgaccgata
     7081 ggccgaggat gcccgggcat aaaggaggat atcgggtgtg accttgtagc tggccgaacc
     7141 caaccagccg agattgtccc aatcatcctt gcgcgactgg gtcgtggtgg tggtgacgat
     7201 cgttgtcgag accgacatcg aatcgagggt tttcttgtct ttcgtgtagc ggatgccgcc
     7261 gaccagctcg agccggccgt cggcgaacgc cggcttccat cccgcctgcg caaaggcggc
     7321 ataggatttc gaggcgatcg aaaagctggt gcttcggtcc agccggatgg ccagcgtcgg
     7381 cgagagcggc gaattgagga tcgtattgat cgtctcgtcg accttctcgt cataataata
     7441 gaggccgaca agatagttga aatcgccgac atcgccgagg aattgcaact cctgcgtcaa
     7501 ctgcttctgc tggcccgggt tcgaaggcgt gacgtgcgcc gtcacccatt cgatcggctg
     7561 gccgggaacc gccggattcg cgaccgcgcc gcgcgtcgcg ccgccgccga gattgccacg
     7621 caaaacctgg ttgatctcgt tatagccggt gatcgacttc accgtcagcg cgtcgctgaa
     7681 atccttttcg agcgtcagga cgccgccata gctttctacc gacgattcac cgttggtgcg
     7741 cgggtcgcga tagctgaaat cgcgcggacc aaccccgatc ggatatgcgg gcccattgac
     7801 ggtgccggag ccctggaaat aggccgaccc gaccgcggag ccggcgagcg cttcccagct
     7861 ggtgtaggaa ttgaccttgt tatagcgacc ccgcagatcg acgatgaaat cgccgccgag
     7921 ttcgccgcgc agcgcagcac cgacgccata gctgtaaagc gcgccgcccc atttcgattc
     7981 cgaaaccccc ggcgattcaa cccagccatc gcgcgagtga acctggcccg acatcttgaa
     8041 cgaaaaaggc gaattgccga gctgcccgaa ctgcacgacg ctcgacgccg tgatctcgtt
     8101 acgcgagccg taggagaaat tggcctggac gccgaactcg tcctcgggat tgcgcgtcgc
     8161 gagcaggatc gcaccgcccg tcgtgttgcg gccgaagagc gtgccctgcg ggccccgcag
     8221 cacttcgacg ctcgcaatgt ccggcaaatc gaaggtgttg ccgctgggac gcggtgtata
     8281 aacgccgtcg agatagacgc cgaccgccgg ctcgacatag agcgcggtcg aaagcgtgcc
     8341 gataccacgg atatagaccg cgggcgcgtt gccgctgccg ctcgcctgcg tgatgcgcag
     8401 gctgggggcg agctgggtca acccgtcgat gcggacgatg ttgaggtccg aaagctgatc
     8461 cgaagaaatc gcggtgatcg acaccggcgt ttcctgtgcg cgctcctcgc ggcggcgcgc
     8521 ggtaacgatg atgtcttcaa cgccgccttg gcgagccatc gccggctggg tgtccgccgg
     8581 agcgggttcg ggctgaacat cctgcgcgaa agccgctgcc ggcatcgtag ccgctagtgc
     8641 cgccgccatg gccgctccac tcgtcgtcca ggcacgacgc acaaaatcca ttgccgcttc
     8701 cgtctggtcg ctcttcaccg cagtttctcc catttctttg tatttctgcc agcatgacgg
     8761 tcacgcggaa gaaggacaaa tctgaaacgg gcacaatagc gtttccaaac gggcatcgga
     8821 ggatgccgct tttcgaaccg cccggcgggt agcacatact gctcattaca ttctggcgac
     8881 cctctcccag ccgggcattt ttccagatcc acgggacgat cgatcccgca cccgcaaatt
     8941 tgcgaacggc agctttcagg accgaaacgg gaccagccgg tgaccagtgt caggcgcaaa
     9001 gttgccgtgc ccgaacatga gaaatccgag caaccggcga gcatgtttgg cagcagtcgg
     9061 tgtgtttgga agcatttcca atgacggcag cggtcgatct tcgaagatct tcagaatgct
     9121 tctaaagcat tgaaatcatg gatccagcgc ttccttcgat tcggaccgcg gcgcgtcaaa
     9181 attcagcgcg taatagcatt gcttcacgaa cgaggtgctt aggtggcaaa atactggctc
     9241 ccggacccac atccggaacc caaccaagca attgaaatat aagaagaaat ggcgcacccg
     9301 aagggattcg aacccctggc ctctgccttc ggagggcagc gctctatcca gctgagctac
     9361 gggtgctgat agagcgcggc ctctagcaag cccttggccc gccagcaatg gcctatcgac
     9421 cgtggcgcgc cgatatgcga agcggtccgc gcaagaggtg gcgcgaagag ggcaagggag
     9481 cgttggtgaa accgtccgaa cagagctggc cgatggcggc cgacctttac ggcgagatgc
     9541 agctcgaggc gaatccgccc gcgcggcatc gctggcgcga ctatcttccg ggcattctgg
     9601 tcaccgcgat cgccgcgctc gccgccgcgt ggctcgccga ccattatgcc gcgccgatcg
     9661 tgctgatggg gctgctcatc ggcctcgcgc tgtccttcct gtcgcaggac aggcgcaccc
     9721 acgccgggct cgacctgatg tcgcagaccg cgctccgcat cggcatcgtc ctcgtcggcg
     9781 cgcggatcac cgccgggcag ctcgccgagc tcggaccgct gcccttcgcg ctgctcgtgc
     9841 tcatcatgct cgccgtgatc gttatgaccg tcgccaccgc gccgctattc cggcaggaca
     9901 agcaagccgc gctgctcgcg ggcggcgcga ccgcgatctg cggcgcgtcg gcggcgctcg
     9961 cgctctattc gctgatcggc gacaagcgca tcgaccaggc gcgcttcacc ctcaccctcg
    10021 tcggcatcac cgtcgcgagc gcgctggcga tgacgctcta tcccgtgctc gcggcgcagc
    10081 tcggcctcag cgacgcgcag gcgggcttcc tgatcggggc gtcggtccac gacgtcg
//
LOCUS       DATMSU010000108        21448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172659,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000108 DATMSU010000000
VERSION     DATMSU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 21448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21448
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172659"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1042
                     /locus_tag="VN034_02880"
     CDS             365..1042
                     /locus_tag="VN034_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV59571.1"
                     /translation="MRVLIVEDNRRLAALLSHAMAQHGFSGDGVGSIEEAEAALASVA
                     FDVVILDLGLPDGDGLQWLRRDRPGNPLPPVLILTARDGMGDRVAGLDAGADDYLVKP
                     VDVEELAARLRALLRRPGKRGLPIIRVGKLAFDVAARTARSEGHSIELTRREADLLEL
                     LMRRAGSVVRRSTIEEALYRFDEIVTPNAVEAIVSRLRHKLSEADNPDSLITVRGMGY
                     LLKESGH"
     gene            1080..2087
                     /locus_tag="VN034_02885"
     CDS             1080..2087
                     /locus_tag="VN034_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWV59572.1"
                     /translation="MAWTGVFGSLALIIFVSFDVEVMLEQQSAAENWHGVWLEIGEHV
                     VLPLLLLMVPMFFAARWVIRTSIEPLSAAAARMDSVVGTDRGFRVDIEEFPLEAQPFA
                     RALNDLLARLDHAAGLREAFAANAAHELRTPLAILMIELDRLGSPDALRLKKDVAALK
                     RLVGQIMMMAQVEAYEAAPTSRNMVSLLEVASDVVNRFAPLAVEQGRQVELAVDGEPT
                     VYGIREMLTAAVSNLVENALRVTPAGGSVVISVGPDARICVRDEGGGLSASDLASLSQ
                     RFVRADHASSNGAGLGLAIVAGIVEIHDAALMTDPDRKEICLLFQNVPHDSEELMSGH
                     A"
     gene            2248..3003
                     /locus_tag="VN034_02890"
     CDS             2248..3003
                     /locus_tag="VN034_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV59573.1"
                     /translation="MANEAAQILIADSHPLCRQGLTTVFTQNLGLSNLAEALDFPSVM
                     AKIAAQRSIGFVTIDLGLPGMRKREGLRDLRVKYPEVQVVVVTASRDREMVLDALGAG
                     AHGYIPKDLPVHEMADALQKVLAGQIYVPPFLSDLNVRKTSIPDEDGGEYETALTGRQ
                     LEVLSLLAAGRSNKEIARLLCIAEGTVKVHIAAAFRMLGVHNRVSAVAVMQARAFDDG
                     AAETYLPGLIEVKRERSISQRNFSGLSRIAYRR"
     gene            3309..8957
                     /locus_tag="VN034_02895"
     CDS             3309..8957
                     /locus_tag="VN034_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWV59574.1"
                     /translation="MVTYIRSDLDFILEQIKISEAHARWTQDPSDPLGRPLFAPDPDG
                     AGPLQGGSIPSYNIAWGLRTVDGTFNHLLPGTEEQGASDNPFPRLLDPVYRPAQGGML
                     DPDGPGPAPAFPTAPNYNPSNNPNNVVIDSSVRTISNLLVDQTLSNPAAIISALQRNG
                     IEGDLIAIAQSIKAEYEPIKSFFKDVDDATDAYADAQAAADAANPPDPALDAAAAAAL
                     ADLTAAQDALTAVRGPLDALLAPNGIELSGANVELPNIAPDEGLSAPFNSWFTLFGQF
                     FDHGLDLVAKGGSGTVFIPLQPDDPLYDPTSPTNFMVLTRATVGAGADNIMGTADDVQ
                     PLNTTTAFVDQNQTYSSHSSHQVFLRQYELNAAGDPVATGKLIEGVNGGMATWANLKA
                     QAADLLGIQLLDVDVGNIPLLATDEYGNFIRGPNGFPQIVMAGLNPGDPPFLVEGDPA
                     ANGGLGVLVAGAVRTGHAFLADIAHNAVPDGLEDGDTEIGGNIPADGEYDDELLDAHF
                     MAGDGRANENIGLTAVHHVFHSEHNRLVEHTKAVVLATGDAAFIAEWQMPDGSWNGER
                     LFQAAKFGTEMQYQHLVFEEFARKIQPAVNTFLVPDGFDITLDPSIVAEFAHVVYRFG
                     HSMLTESIDRFDPTFNEEHISLIQGFLNPLQFENNGGNAGVDADIAAGAIIRGMTRQA
                     GNQIDEFVTSALRNNLLGLPLDLASINLARGRDTGVPSLNAARAEFYAATNEDELLKP
                     YESWVDFAANLKNEASIINFVAAYGTHTLITSQTTIEGKRDAALTIITGVSTGGHLVP
                     EDRLDFLNATGAYAGGTLGGLNNVDLWIGGLAEKTMPFGGMLGSTFNFVFEVQMENLQ
                     SGDRFYYLQRLDGLHLFGEMENNSFAAMIMRNTDATHLPSDVFSTPGLILEVDPTRQF
                     NDLDGDGTLEGGDPLGGGILTPLVSRNNPGTAGSDTNYLRYTGGDHVVLGGTDNADIL
                     IGGIGDDTLHGDGGNDRLEGGFGNDIINGGAGDDFIKDTGGDDNIKAGDGNDIVHAGP
                     GLDLVLGGRGNDFIFLGTDEGSEVFAGEGDDFIYGNKNAERILGNEGSDWIETGTFDG
                     APGDNFDEVFSRDGIDGHDVFLGDGGFDEFIGEGGDDIMVGSAGRGKMVGMSGFDWAT
                     YKDNTSGVNADLSIPIIFDEAPTIPGNAALDEFETVEGLSGTAFNDILRGTNDTAAEM
                     LPIPQGGTGGYRGSELDADGIARISGLQAVVGAGVTSFAAGDIILGGDGSDLIEGQGG
                     DDIIDGDKYLNVRIGVFQNNDGTGAQIDTANSMTELASEMFNGVYNAGQLRIVREILT
                     DTTAGDTDIAVFSDLRANYDITHNANGTISVAHARGTQTDGTDSLRNIERLRFADGEI
                     ATNEPATGQPVISDLTPTEGQTLTLNLAGIADANGLGAFSYQWQQSLDGLTWVNIAGA
                     TNASFTPDDNNNQIFGDQAGQRLRVTVSFVDGVGASESVTSAATGPTGVNWLGSGGNN
                     TLNGTAGNDIADGAGGNDTLNGNGGDDILNGAAGNDTVSGGLGNDTLMGAAGNDTLTG
                     GAGNDSIAGNAGNDTVVFSGPIGNYNIETVGTALVVTDNTGADGVDTVNNTGAFRAEV
                     LRFNGVDYANVTGTAANNNNLNGAGGSAGSQAIFGMAGADTINGGAGNDYIHAGSGND
                     TITQTGSTGGRDIVNGSAGTDTFVLNGVAGAETFRIYARAAAEAAGITGLNANTEIVV
                     TRNGTANAQVIAELDNIEEINVNALNVSSPGGVPPGGSGLNGGDTILVIGDFNPTSLN
                     FSTITVNGGRSADTVDISGLTSDHRLVFNTGGGADRFLGDERPQDVVNSGHGNGNGGG
                     GSAHRTDEPFQLLRSLIDNWMEDHGRFSFRHHFEADYPFN"
     gene            9024..10778
                     /locus_tag="VN034_02900"
     CDS             9024..10778
                     /locus_tag="VN034_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWV59575.1"
                     /translation="MRLFWMPVPPALSEAVRACRTHFVLAATYSALINILYLAPTIYM
                     MQVYDRVVPTNGVLTLVFITAVVGVAIATLSALDAVRVRLMTRASLRLDRLLSGEILD
                     RLLARSRARPGEPSTQQAMREFDLLRQSLAGQAATALFDVPWTPLYLFVAFLIHPLLG
                     LLVLGAGSILVVLAITNERRSKAKAEEAHQASAAAYESHEAMLRKAEIVRSLGMRRAL
                     VSRHIQQRSVGLNVVADLQFSGSRYNGLVKFVRMFMQSFALGVGAWLAINGQISVGAI
                     IAASVLLSRALQPIEQLVGLWPNIVQSRQAIQTLGRLFEQATGPVVRTTLPDPQGQVD
                     LQGIVVRNAEGSAILLKNISMKLSPGEVLGIIGPSGAGKTTLARVVAGALPPDLGEIR
                     VDGASLADWDPEQLARHIGYLPQDCGLLPGTISENISRFSASCGVAQAVVDELVVKAA
                     RTAGVHEMILRLPSGYDTNIEGNGHRLSAGQGQRVALARALYGDPRILVLDEPNSALD
                     SDGEEALSRALGAARLQGAAIMIVAHRTAVLGCADKLAILADGAIVGMGPRDEILDAL
                     QKSAAKQNVVPIHEGARP"
     gene            10775..12136
                     /locus_tag="VN034_02905"
     CDS             10775..12136
                     /locus_tag="VN034_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWV59576.1"
                     /translation="MTDMSWKPPSGGDRPSDSDSADRPHRELRIGALIIAFFFVGLLG
                     WASLTPLDAGAYAPGVVAVSGSRQAVQHREGGIVTELHVVEGQTVKKGEPLLTISASE
                     LVATERGLTGEVTALLAQRARLVAELGGLSSVAEPAEFASLAPEDRGLAAEALRGQRQ
                     LFEARRNSVRTQRNVLRQRMRQHSEQINGYSYQMNSNKVQQQLIGEELDGLRTLLPKG
                     FVSINRVRSMERAAAELDGSYGAYRADIARSSEAIGEAQMQIVGIDKQMLEEVATAKQ
                     AGQVRLDELQPKLIAVREQLARSTVRAPAGGRVVGLKIFTVGGVVSAGDTLMEIVPQD
                     RALVIDGKASPTDADDLAPGMQTQIRFTALQERNLPILVGQISKISADSFEDERSGLR
                     YFKIELVVPPSELAKLRRVRADGGLRAGLPVDIMIPLRKRTALSYLIEPLTQTLWMAG
                     REN"
     gene            12516..13196
                     /locus_tag="VN034_02910"
     CDS             12516..13196
                     /locus_tag="VN034_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV59577.1"
                     /translation="MRILIVEDNARIGALTAEGLSAMGFCCDIAPTLAEASELLAATS
                     YDVLVLDLGMPDGDGRAWLMVERAQGLATPVIMLTARSALGDRIDGLDAGADDYLIKP
                     FAIEELAARVRALLRRPGARSSALLAVGPIQFDTIARTATCSGEPLHLSQREAALLEL
                     MMVKAGSVVSRDQIEASLYPFDDPVTPNAIEVLVSRVRKKLATLGVNDQLVTLRGVGY
                     LMIEPVGG"
     gene            13193..14218
                     /locus_tag="VN034_02915"
     CDS             13193..14218
                     /locus_tag="VN034_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWV59578.1"
                     /translation="MTGGRSIIAELRSGFAWMAAAGSIVLAAFLVVDFELLQAEIRAT
                     YGRIGIAHEVVDHVIFPIIVLLAPLAIATRWLIGRALRPLHDAADLIHQATGRERGFR
                     VRLADFPTEALPFAEAVNSLLSRLDDAARRQEGFAADVAHELKTPLAVAMLELERLKT
                     ADTDRVIADLAAMNRLVEQLLILAQLDAYAAAPQPFRPIDLRDPALEAIKLCAVDAAQ
                     KGVALAYRDEGAAAPVMGRIEAVAAALRNLVENAIRVTAAGGEVVIVRGPGPSIRVAD
                     GGAGIDPDRLLTLSDRFRRGEHASFGGAGLGLSIVARIMEVHGGTMRSHPDEPSIELD
                     FGANVAS"
     gene            14401..15111
                     /locus_tag="VN034_02920"
     CDS             14401..15111
                     /locus_tag="VN034_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV59579.1"
                     /translation="MTALVWERQLLCRSGLASIVRREFANIEVATAGDADQALAWLAA
                     TDAPGLLILDGGIDLASVVPYGIRGLRRRYPRLALVIVDWRRDRDAVLGAIGEGAHGF
                     IQKDMEQDEMLQAFRQVLAGQVYVPPIVSDVDTDMPVAGSSGSQQVSITSLTERQREV
                     LTHMSMGKSNKEIARSLRISESTVKVHVAAAFRLLGVRNRVGAVAVIQGRSGASAFMR
                     DHLLGARRAYEPRKHAFG"
     gene            15441..>21448
                     /locus_tag="VN034_02925"
     CDS             15441..>21448
                     /locus_tag="VN034_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWV59580.1"
                     /translation="MVKLTQHDLNFILQQIEIAEEHNRLVQEDGMDPGEALRLLVDNP
                     LLPNGLRTVDGSFNNLVPGRELWGASGEAFPRLTTPNWVNESDDSIIFGAGSPGQISF
                     TDGNYGENGPPSGPGGLGGGTLVDADPRTISNLIVDQTLNNPAVIAAALRLAGHAESE
                     IMDGIAAIRTAMQAHPDALEALAGANLALTDAQAAYNQAVANLIAETDPAAIPALITA
                     VSTAATAQAEAQVAVTTAEDAVEVAEGVLSATLDDYGVEMDGNSVLLRNVSPDEGLSA
                     SYNGWFTLFGQFFDHGLDLVSKGGNGTVYIPLQPDDPLYNPDSPHTNFMAMTRATVGV
                     GAGNVTTPWVDQNQTYTSNASHQLFLREYEMVGGKPVSTGHLLEGDRGMATWADVKEQ
                     ARTMLGIELTDADVTQVPVFRMDPYGEFIRGPNGLPQILAAFTPEGEPIYVEGNLASP
                     INPSAIQLPVGTVIMGSGGDNVIEAGETVSAARTGNAFLDDIAHAAVPVVAGGVLQQD
                     ADDAVGYSGGFNTRGQQTQYDDEMLDAHYITGDGRGNENIGLTAVHHVFHSEHNRQVD
                     EIKKTAFESGDLAFLNQWLATDVTSIPATMDGLVWDGERLFQAARFSNEMQYQHLVFE
                     EFARKIQPDVDIFLVEPDVELNPAIFAEFANVVYRFGHSMLNQTVDQQNADGTVTNME
                     LFDAFLNPLAFGSDTVDHREAAAAIVRGMTMQVGNEIDEFVTDVLRNQLVGIPLDLAA
                     LNIARGRDTGMPTLNQARAQFQDMATGDTLLRPYTSWTDFALNMRNPESVVNFIAAYG
                     THATITAETTIEGKRDAAMALVFGGEGAPGDRLDFLNATGAYAGGSLGGLNDVDLWIG
                     GLAEKKMAFGGMLGSTFSFVFQMQMENLQDSDRFYYLSRTQGLNLLTELENNSLAKMV
                     KKNTDLDGFALPADIFSTPDHILYMDYNKQLAMTNTADPVHENAVLQGISSLVERRDV
                     DGNGVAEYIRYNGLDHIVIQGTSANDHIVAGGGDDTVWGEDGDDRIEAGYGVDSINGG
                     AGDDIITSAGTDIGAVSVLKGETGNDVIVDGTGMSLIFGGDGKDYIVSGFDDGEIRGG
                     QGGDFIYGGDGMGMLFGNEGNDWVEGGGGFDFIAGDMAELFFNSTIIGHDVLNGGQGD
                     TDYDADSGDDIMFGGEGIQKNIGMWGHDWVTHQGQSSGVDADMRVDIFTTLPNEVLRD
                     RFSQVEALSGWNGNDVLRGDDRTGADAEGGTTTDPTPETGFRYNELNQAGMDRIAGLS
                     EIITPDLMQTLPYWATGSWEDGATGTTEAVFVGGNILLGGGGSDTIEGRGGDDVIDGD
                     RYLNVKLTGFTKDGIAFSVTSMEDSVTLGGVTKSLTSWMIDGRINPGTMEIVREILSD
                     DSGEDTAVFWDVLENYAITSNGNGGLRIEHVDQTGGAIDPVTGRNREADGIDQVYNVE
                     WLQFGDQRISTAGLINTPATGAPVISDLTPTKGQALTANVGSIQDENGLGTFGYQWQV
                     SSDNGATWNNVVTGGTGASFTPTGGQVGGLMRVSVSFTDGAGHQETLVSAPTGVVGDN
                     WDAIPFLANTFNGTAGDDIADGTNGLFGAGAADTLNGNGGNDILSGAGGNDTLNGGAG
                     DDRLDGGAGNDVAVFAGNVANFALASNGTNLVVTDLTGAEGTDTLSTIETLRFAGTNY
                     SVVAGTGANNANLNGASGAAGSQAVFGFAGTDMLNGGGGNDLLVGGQGNDTVNGGAGN
                     DTAYWSVGDGRDFVNGGADTDTVKVVGDASAETFRIYSRAAALAAGLTGLNANAEIII
                     TRNGTTNADIIAELDNVEEIVINGRGGGDTFLPIGDFAPTSLSTSTITIEGSDGDDVV
                     DIGALQSAHRVVFKSSGGDDVILGQMRDQDRFELADGKTIDDYTATENADGSTTLSTE
                     GHSVTFFGTTANAGGAAASDDEDHGHDNDHDDDDTPPPSDADTLTGTAGRDVLNGGSG
                     DDELEGRGGDDTLSGGSGDDVLKGGGGDDMLSGGSGDDALSGGAG"
BASE COUNT         4118 a         6581 c         6904 g         3845 t
ORIGIN      
        1 gacccgatcc gcgcgaaagg catcatggcc agcggcgcat gacaaggccg catcacaggc
       61 cggacacatg gctgcaccaa ccagcaggtc gcatcacatc cagaaaccac ttgccaacgg
      121 agggccgtcc acacacggcc aaactcagcg gtagcgtgtg cgggcaagtt ccagaatgaa
      181 cgagcgaatc aaattgggtg aaaacggaag gccaaatttg ttgagggctt cggattttct
      241 tgattccgta gataatgagt tcatattccg cggggcttgc acagaacagc actgacctct
      301 gtgggtgtcg aggttttcgt gccgggctca gtcaggggag ggctacgctg atagctggtg
      361 cgacatgcgc gtcctgatcg tggaggacaa tcgccgtttg gcggcgctcc tgtctcacgc
      421 catggcccag cacggattta gcggtgacgg tgtcgggagt atcgaggaag ccgaggcggc
      481 gctggccagc gtcgctttcg atgtcgtcat cctggatctg ggattgcccg atggcgacgg
      541 cctccaatgg cttcgccgcg accggccggg aaacccgctc cctccggtcc tcatcctgac
      601 ggcgcgtgac ggcatgggcg accgggtcgc cggtcttgat gccggcgcgg acgactattt
      661 ggtcaagccg gtcgatgtcg aagagctggc ggctcgtctg cgcgcgcttt tgcgtcgccc
      721 cgggaagcgc ggcctgccga taatccgggt cggcaagctc gctttcgatg tcgcggcacg
      781 gacggcgcgc agcgagggac attctatcga gctgacgcgc cgcgaagccg atctgctcga
      841 actgctcatg cgcagggcag gctcggtcgt ccgccgatcg acgatcgaag aagctctgta
      901 tcggttcgac gaaatcgtta cgccaaatgc ggtcgaagcc atcgtgtcgc ggcttcgtca
      961 caagttgagc gaagccgaca atcccgactc gctcatcacc gtccgcggga tgggttattt
     1021 gttgaaggaa tcaggccatt aggacgtcgg attccataac gggtcgcctg acgcgcgggt
     1081 tggcatggac gggcgtcttc ggcagcctcg cgctcatcat ttttgtcagc ttcgatgtcg
     1141 aggtcatgct cgaacagcag tcggcggccg agaattggca tggtgtatgg ctcgaaatcg
     1201 gcgagcatgt cgtcttgccg ctcctcttgc ttatggtgcc gatgttcttc gcggcgagat
     1261 gggtcatccg aacctcgatc gaaccgctta gcgcggccgc ggcccgcatg gattcggtgg
     1321 tgggcaccga ccgcggattt cgcgtcgata tcgaagaatt ccctctcgag gcgcagccat
     1381 tcgcgcgggc gttgaacgat ctattggcgc ggcttgatca tgcggcggga cttcgagaag
     1441 cctttgccgc caatgccgcc cacgaactca ggacgccgct ggcaatcctg atgatcgaac
     1501 tggatcgatt gggttctccg gatgctctaa ggctgaagaa ggacgtggcc gcgctgaaac
     1561 ggctcgtcgg ccagatcatg atgatggcgc aggtagaggc ttatgaggcg gcacccacct
     1621 cccggaatat ggtctcgctc ctcgaagtcg cgtcggatgt ggtcaaccgc ttcgcaccgc
     1681 tggcggtcga gcaaggcagg caggtggagt tggctgtcga cggcgagccc acggtttacg
     1741 gaattaggga gatgctcacc gccgccgttt cgaatctggt ggaaaacgct ttgcgggtca
     1801 ccccggcggg cggcagcgtt gtgatttccg tcggcccgga tgcccgcatt tgcgtcaggg
     1861 acgaaggggg aggtctgtcc gccagcgatc tggcgtctct cagccagcgg ttcgttcgtg
     1921 ccgatcatgc gagctcgaat ggagcgggtc ttggcctcgc gatcgtggcc ggtatcgtgg
     1981 aaattcacga tgcggcactg atgacggacc cggaccggaa agaaatatgc ctgctatttc
     2041 aaaatgtgcc gcatgatagc gaagagctca tgagcggaca tgcttagctg tcaggttggc
     2101 gtcaggttcg tggcataggc tcgactggac cgattcgttt cttaactaaa aaatagcctt
     2161 cccgcacggg tcgcggcggc gggaaggagt catgcgggat gtgtttgtcg gcgcgtgcct
     2221 aggcaaatac ccgtgggggg caaatccgtg gcgaacgaag cagctcagat tttgattgcg
     2281 gatagccatc cgctttgccg ccaaggactg acaacggtct tcacgcaaaa tctcggcctg
     2341 tccaatttgg ccgaggcttt ggactttccc tccgttatgg cgaagatagc ggcgcaacga
     2401 tcgatcggct ttgtgacgat cgatcttggt ctgccgggaa tgcgcaaacg tgaaggcttg
     2461 cgtgacctgc gcgtgaaata tcccgaagtg caagttgtcg tcgtaacggc ctcgcgggac
     2521 cgcgagatgg tgctggacgc gctgggcgcc ggcgctcatg gttatattcc caaggatctt
     2581 ccggtccacg agatggcgga cgcattgcaa aaggtgctgg cggggcagat ctatgtccct
     2641 ccctttcttt ccgatctgaa cgttcgaaag acaagcatcc ccgacgagga cggcggcgag
     2701 tatgagacgg cgttgacggg gcgccagctc gaggtcttga gcctccttgc ggcggggcgc
     2761 tcgaacaagg aaatcgcgcg gctgctctgc attgccgagg gaacggtcaa ggttcacatc
     2821 gccgcggcat tccgcatgct gggagtgcac aaccgcgtca gcgcggtcgc cgtaatgcag
     2881 gcgcgggcgt tcgacgacgg agctgccgag acctacctgc ccggccttat cgaagtgaaa
     2941 cgcgaaaggt cgatatcgca gcgcaatttt agcggactgt cccggattgc gtacaggcgc
     3001 tgaggcgctt tttaacaccg cccgaaatca tcttcccttt cgactttgtt caggcgcgcg
     3061 ccgaagggcg catagtctcg tcttccgcgt cgggcgctat atcaaaagta ctatatacaa
     3121 tatgacgtcg atatataccg cgtgatatag aaagacggaa acgtaatagg tcgccagtcc
     3181 aactcgacga ccatgtttga agagccataa cttacagcct caaaatccgc agatcgaccg
     3241 ccaattgcga gtcgaagatc gatccgacga gtgggcggag ggtgcggatt tactgcaggg
     3301 aggtaagcat ggttacgtat atccgcagcg atctcgactt catccttgag cagatcaaga
     3361 tttccgaggc gcacgctcgg tggacgcaag acccgtccga tccgttgggc cgacccttgt
     3421 ttgcgcccga tccggatggt gccgggccgc tgcagggcgg ctccatccct tcctacaata
     3481 tcgcctgggg gctccggact gtcgacggaa cctttaatca tctgctcccg ggcaccgagg
     3541 agcagggggc gtccgacaat ccgttcccgc ggttgctgga tcccgtctat cggcccgcac
     3601 agggcgggat gctcgacccc gacggacccg gaccggcgcc ggcattcccg accgcgccaa
     3661 actataaccc ttcgaacaat ccgaacaatg tcgtcatcga ttcgagcgtc cgcacgattt
     3721 ccaaccttct ggtcgatcag acgctgagca atcctgccgc catcatttcg gcgcttcagc
     3781 gcaacggcat cgagggcgac ctgatcgcga tcgcgcagag catcaaggcg gaatatgagc
     3841 cgatcaagtc gttcttcaag gatgtcgacg acgccaccga tgcctatgcg gacgcgcaag
     3901 ccgcggccga cgctgcgaat ccgcccgatc cggcgctgga tgccgcagcc gctgccgccc
     3961 tggcggatct gacggcggcg caagacgcgc tcaccgccgt ccgcggtccg ctcgacgcgc
     4021 tgctcgcccc caacgggatc gaactgagcg gcgccaatgt cgaactgccg aacattgcgc
     4081 ccgacgaggg cttgtcggcg ccgttcaact cgtggttcac cctgttcggc cagttcttcg
     4141 atcacgggct cgatctcgtc gccaagggcg gcagcggtac cgtgttcatc ccgctccagc
     4201 cggatgatcc gctttatgat ccgaccagcc cgaccaactt catggtgctg acgcgcgcaa
     4261 cggtaggagc cggcgcggac aacatcatgg gaacggcaga cgacgtccag ccgctgaaca
     4321 cgacgacggc tttcgtcgac cagaaccaga cctacagctc gcattcttcg catcaggtgt
     4381 tcctgcgcca atatgaactc aacgcggcgg gcgatccggt cgcgaccggc aagctgatcg
     4441 agggcgtcaa cggcggtatg gcgacctggg ccaatctcaa agcgcaagct gccgatctgc
     4501 tcggcatcca gctgctcgac gtcgatgtcg gcaatattcc gctgctggcg accgacgaat
     4561 atggcaattt catccgcggg cccaacggct ttccgcaaat cgtcatggcg ggcctcaacc
     4621 ccggcgaccc gccgttcctg gtggagggcg atccggccgc caatggcgga cttggcgtgc
     4681 tggttgccgg cgcggtccgg acgggccacg cattcctggc agacattgcg cacaatgcgg
     4741 tgcctgacgg ccttgaagac ggcgataccg agatcggcgg caacatcccc gccgatggcg
     4801 aatatgatga cgaactgctc gacgcgcact tcatggccgg cgacggccgc gcgaacgaga
     4861 atatcggcct gaccgcggtc caccatgtgt tccattcgga gcacaaccgg ctggtggaac
     4921 acaccaaagc ggtggtgctg gcgaccggcg atgcggcgtt cattgccgaa tggcagatgc
     4981 cagacggatc gtggaatggc gaacgcctgt tccaggccgc gaagttcggc accgagatgc
     5041 aatatcagca tctcgtgttc gaggaattcg cgcgcaagat ccagcctgcg gtcaacacct
     5101 tcctggtacc cgacggcttc gacatcacgc tcgatccgtc gatcgtggcc gagttcgcgc
     5161 acgtcgtgta ccgcttcggc cactcgatgc tgaccgagtc gatcgaccgc ttcgatccga
     5221 ccttcaacga agaacatatc tcgctgatcc aaggcttcct caacccgctc cagttcgaaa
     5281 ataacggggg caatgcgggc gtggacgccg atatcgcggc gggtgcgatc atccgcggca
     5341 tgacccggca ggccggcaac cagatcgacg agttcgtgac gagcgcgctc cgcaataacc
     5401 tgctgggact gccgctcgac ctcgcgtcga tcaatctcgc acgcggtcgc gatacgggcg
     5461 tgccgtcgct caacgccgcg cgcgccgaat tctacgcggc gacgaacgag gacgagctgc
     5521 tcaaacccta tgaaagctgg gtcgatttcg cggctaacct caagaatgag gcgtcgatca
     5581 tcaacttcgt cgctgcctat ggcacgcaca cgttgatcac gtcgcaaacg acgatcgagg
     5641 gcaagcgcga cgccgcactg acgatcatca ccggcgtgag cacgggtggc catctggtcc
     5701 ccgaggaccg gctcgacttc ctcaatgcga ccggcgctta tgccggcggc acgctgggcg
     5761 gcctcaacaa cgtcgacctg tggatcggcg gcctcgccga aaagaccatg ccgttcggcg
     5821 gcatgctggg atcgaccttc aacttcgtgt tcgaagtcca gatggagaat ctgcagagcg
     5881 gcgaccgctt ctattatctg cagcggctcg acggcctgca cttgttcggc gagatggaga
     5941 acaatagctt cgccgcgatg atcatgcgga acaccgacgc gacgcatttg ccgtccgacg
     6001 tgttctcgac gccaggcctg atcctcgagg tcgatccgac tcggcaattc aacgatctgg
     6061 acggcgacgg cacgctggaa ggcggcgacc cgcttggcgg cggcatcctg acaccgctcg
     6121 tcagccgcaa caatcccggt acggccggtt cggacacgaa ttacctgcgc tacaccggcg
     6181 gcgaccatgt cgtgcttggc ggcaccgaca acgccgacat cctgatcggc ggcatcggag
     6241 acgatacgct gcatggcgac ggcggcaacg accggctgga aggtggtttc ggcaacgaca
     6301 tcatcaacgg cggcgctggc gacgacttca tcaaggacac gggcggcgat gacaatatca
     6361 aggccggcga cggcaatgac atcgtccacg cgggtccagg cctcgatctc gttcttggcg
     6421 gccgcggcaa cgacttcatc ttcctcggca ccgacgaggg ttcggaagtc tttgcgggcg
     6481 aaggcgacga cttcatctac ggcaacaaga atgccgaacg catcctcggc aacgagggca
     6541 gcgactggat cgagacgggc accttcgacg gcgcaccggg cgacaatttc gacgaggtct
     6601 tctcgcgcga cgggatcgac ggccatgacg tcttcctggg cgacggcggc ttcgacgagt
     6661 tcatcggcga aggcggcgac gacatcatgg tcggcagcgc cggccgcggc aagatggtcg
     6721 gcatgtccgg gttcgactgg gcaacctaca aggacaacac gtccggggtg aacgccgacc
     6781 tgtccatccc gatcatcttc gacgaagcgc cgaccattcc cggcaacgcg gctctcgacg
     6841 agtttgagac cgtcgaaggc ctgtcgggca cggcgttcaa cgacatattg cggggcacga
     6901 acgacacggc cgccgaaatg cttccgatac cgcaaggcgg taccggcggc tatcgcggca
     6961 gcgagctcga cgccgacggc attgcccgca tcagtggcct acaggctgtg gtcggggctg
     7021 gcgtcacgtc tttcgcggcc ggcgatatca tcctcggcgg cgacggcagc gacctgatcg
     7081 aaggtcaagg cggcgacgac atcatcgatg gcgacaaata tctgaacgtg cggatcggcg
     7141 tgttccagaa caacgacggc accggcgcgc agatcgacac cgcgaacagc atgacggaac
     7201 tcgcttcgga gatgttcaat ggggtctaca atgccggcca gcttcggatc gtcagggaaa
     7261 tcctgaccga taccacggcg ggcgatacgg atattgccgt attctccgac ctgagagcca
     7321 attacgacat cacccacaat gccaatggca cgatcagcgt ggcgcacgcg cgcggaacgc
     7381 aaaccgatgg cacggacagc ctgcgcaata ttgagcgcct gcgtttcgcc gacggcgaaa
     7441 tcgccaccaa cgagccggcg accggccagc cggtgatcag cgatctgaca ccaaccgaag
     7501 ggcagacgct gacgctcaac cttgccggca tcgccgacgc gaatgggctg ggcgcgttca
     7561 gttatcagtg gcagcagtcg ctcgacgggc tgacgtgggt gaacatcgcc ggcgctacga
     7621 atgcctcctt taccccggac gacaacaaca accagatatt cggggatcaa gcagggcagc
     7681 ggctgcgcgt taccgtgagc ttcgtcgacg gcgtcggcgc atcggaaagc gtcacctcgg
     7741 ctgctacggg gccgaccggt gtgaactggt tgggaagtgg cggcaacaac acgctgaacg
     7801 gcacggctgg caacgatatt gccgacggtg cgggcgggaa cgatacgctg aacgggaacg
     7861 gtggcgacga catcctcaac ggtgccgcag gcaatgacac ggtttcgggt gggctcggca
     7921 acgatacgct gatgggcgcg gccggaaacg atacgttgac cggtggtgcg ggcaacgaca
     7981 gcattgctgg caatgcaggc aatgacacgg tcgtattctc gggcccgatc ggtaactaca
     8041 acatcgaaac ggtcggcacg gcgctcgtcg ttaccgacaa caccggcgcc gatggcgtcg
     8101 acaccgtaaa caacaccggc gccttccgcg ccgaggtcct gcggttcaac ggcgtcgatt
     8161 atgcgaatgt aacggggaca gctgccaaca acaataatct gaacggcgcg ggcgggagcg
     8221 cgggctccca agccatattc ggcatggccg gggcagacac gatcaacggc ggtgccggaa
     8281 atgattatat ccacgccggg tccggaaacg acacgatcac gcaaaccggg tccacgggcg
     8341 gccgcgatat cgtcaatggc agcgcaggca cggatacgtt cgtgctcaat ggcgtggcag
     8401 gcgcggaaac cttccgcatc tatgcccggg cggctgccga agccgcgggc ataaccgggc
     8461 tgaacgccaa cacggaaatc gtcgtcacgc gcaacggtac ggcgaatgcg caggtcatag
     8521 ccgaactcga caacatcgaa gagatcaatg tcaacgcgct caacgtgtcg tcgcccggag
     8581 gcgtcccgcc cggcggttcg gggctgaacg gcggtgatac gatcctggtc atcggcgatt
     8641 tcaacccgac cagcctcaac ttcagcacga tcaccgtcaa tggcgggcga agcgccgaca
     8701 ccgtcgatat atccgggctg acctccgatc accggctggt attcaatacc ggcggcgggg
     8761 cggatcggtt tctcggcgat gagcggccgc aggatgtcgt caattccggc catggcaatg
     8821 gcaatggcgg cggcggttcg gcgcaccgga cggacgagcc tttccaactg ctccggtcgc
     8881 tgatcgacaa ctggatggag gatcatgggc gcttcagctt ccgtcaccat ttcgaggcgg
     8941 actatccatt caattaacca gcttggcgcc gggatctccc cccggcccgg cgccactttt
     9001 ttccgataat cagggaggat tgcatgcgcc tgttctggat gcccgttcca ccggcattgt
     9061 cagaagccgt ccgcgcctgt cggacccact tcgtcctcgc agcgacatat agtgcgctga
     9121 tcaacatcct ctatctcgca cccaccatct atatgatgca ggtctacgat cgggtcgtgc
     9181 cgaccaacgg cgtattgacg ctggtcttca tcaccgccgt ggtcggcgtg gcgattgcaa
     9241 cgctgtcggc gctcgacgcg gtccgcgtgc gactgatgac ccgcgcgtcg ctgcgcctcg
     9301 accgattgct ctcgggcgag atactcgacc ggttattggc ccggtcgcgc gcacggcccg
     9361 gcgaaccctc cacccaacag gcgatgcggg aattcgacct gctgcggcag tcgcttgccg
     9421 gtcaggccgc aacggcattg ttcgacgtcc cctggacgcc cctgtatctt ttcgtggctt
     9481 tcctcattca cccgctcttg ggattgctgg tgctcggtgc ggggagcata ttggtggtgc
     9541 ttgcgatcac caacgaaagg cgcagcaagg caaaggccga ggaagcgcat caggccagcg
     9601 cggcagcata tgaatcgcat gaggcgatgt tgcgcaaggc tgagatcgtg cgctcgctcg
     9661 ggatgcggcg cgcactggta tcgcgccaca tccaacagcg cagcgtcggc ctgaatgtcg
     9721 tggccgatct ccagttttcg gggagccggt acaatgggct cgtcaagttc gtgcgaatgt
     9781 tcatgcaatc cttcgcactg ggtgtcggcg cctggctcgc catcaacggg cagatttcgg
     9841 tcggcgctat tatcgccgct tcggtgttgc tcagccgtgc tctgcagccg atagagcagt
     9901 tggtcggcct ttggcccaac atcgtccagt cgcgccaggc gattcagacg ctcggcaggc
     9961 tattcgagca ggcaaccggt ccagtcgtgc ggaccacttt gcccgatccg cagggtcagg
    10021 tggaccttca agggatcgtt gtccgcaatg ccgaaggaag cgcgatcctg ctcaagaata
    10081 tatcgatgaa gctttccccc ggggaggtgc ttggcattat cggcccgtcc ggggccggca
    10141 agacgaccct tgcgcgggtc gtcgccggcg ccttgccgcc cgacctcggc gagatcaggg
    10201 tcgatggcgc cagtctggcc gattgggatc ccgagcagct tgcgcggcac atcggctatc
    10261 tcccccagga ttgcggcctg ctacccggaa cgatcagcga aaacatatcg cgtttcagcg
    10321 cctcatgcgg cgtcgcgcag gcggtggttg acgagctggt ggttaaagct gcccgcacgg
    10381 cgggtgtcca cgagatgatc ctgcgtctgc caagcggtta cgacaccaat atagagggca
    10441 atgggcacag gctgtcggcc gggcaggggc aacgcgtcgc tctcgcgcgc gcattatacg
    10501 gcgacccgcg tatcctggtt ctcgacgagc ccaattcggc gctcgacagc gatggagagg
    10561 aggccctgtc gagagccctc ggtgccgcaa ggctgcaagg cgcggcgatc atgatcgtcg
    10621 ctcatcgcac agccgtgctc ggctgcgccg acaagctggc gatccttgcc gatggagcga
    10681 tcgtggggat gggaccgcgc gatgaaatcc tcgacgcttt gcagaaatcc gcggcaaagc
    10741 agaatgtcgt tcccattcat gaaggagcgc ggccatgacc gacatgagct ggaagccgcc
    10801 gagcggcggc gatcgtcctt cggacagcga ttccgcagac cgtccgcatc gcgaattgcg
    10861 catcggcgct cttatcatcg cattcttctt tgtgggcctg ctcggatggg cgtcgctgac
    10921 cccgctcgat gccggcgcct atgcgccggg cgtggtcgcg gtgtcgggca gccgtcaggc
    10981 ggtccagcac cgcgagggcg gcatcgtgac cgaactccat gttgtcgagg gtcagacggt
    11041 caagaaagga gagcctcttc tcaccatctc ggcctcggaa ctggttgcga ccgaacgcgg
    11101 actgaccggc gaagtcaccg ccctcctggc gcagcgcgcg cgtttagtcg ccgaactggg
    11161 cggtctgtca agcgtcgccg aaccggcgga atttgcgtcg ctggcgcccg aagaccgggg
    11221 cttggccgcc gaggcgttgc ggggccagcg ccagctattc gaggcgcggc gcaattccgt
    11281 gcggacgcag cggaacgtat tgcgccagcg gatgcgccag cattcggagc agatcaatgg
    11341 ctacagctat cagatgaatt ccaacaaggt gcagcagcag ttgatcggag aggagctgga
    11401 cggactgcgg accctgcttc cgaaaggctt tgtatcgatc aaccgggtgc gcagcatgga
    11461 acgcgccgcg gcggaacttg atggctccta cggcgcatat cgggccgaca ttgcacgctc
    11521 ttccgaagct ataggcgagg cgcagatgca aatagtcggt atcgacaagc agatgcttga
    11581 ggaggtggcg acggcgaaac aggcgggaca ggtccgcctc gatgagctcc agcccaagct
    11641 gatcgccgta cgcgagcaac tcgcccgatc gacggtgcgc gctccagccg gtggccgggt
    11701 ggtggggctg aagatcttta ccgtcggcgg cgtcgtttcc gccggcgata cgctgatgga
    11761 gatcgttccc caggaccgcg ccctggtcat cgacggcaaa gcttcgccga ccgatgccga
    11821 cgatcttgcg ccgggtatgc agacccagat ccgttttacc gcgttgcagg aacgcaatct
    11881 gccgatcctc gtcggccaga tttccaagat ttcggccgac agcttcgagg atgagcgttc
    11941 ggggctccga tatttcaaga tcgaactggt cgtgcccccg agtgagttgg cgaaacttcg
    12001 gcgcgttcgt gcggacgggg gactgcgagc cggcttgccg gtcgatatca tgatcccgct
    12061 acgcaagcgg accgcgcttt cctatctcat cgagccgctc acccagacgc tctggatggc
    12121 cggacgcgaa aattagtagg gctgtagcta ggccgcgagt gcgcggctct cttgaacggc
    12181 cagacttcaa tcacgtgacg cgacccaatg tcggcgggcc ggtgccatca gcgcacctcg
    12241 ctttttaggc aggtcatcgt aacgggtcag agaatatcaa atatgtgcag caccagccac
    12301 agcaacgata ggccggcaag ggcgcccaga aaccagcctt ctttgcgcat ttcccctccg
    12361 ttcactggcc aattatgaca cctgcaaccc ccgttgtcat aattcaagcg ggacgatgtc
    12421 gcgtccatct ggcctatcgg attaagcctt aggtactaga tcgcggcgct tgaccatttg
    12481 tggtagggat ggtgcacttc gtcgaggagt acaaggtgcg catactcatc gtcgaagaca
    12541 atgcgcggat aggcgctttg acggccgaag gcctttcggc gatgggattc tgctgcgata
    12601 tcgcgccgac gcttgccgaa gcctcagaac tgcttgccgc gaccagctat gacgtcctcg
    12661 tgctcgatct cggtatgccc gatggcgacg ggcgggcgtg gctgatggtc gagcgcgcgc
    12721 aggggcttgc gacgccggtg atcatgctga ccgcgcgttc cgccctgggc gaccgtatcg
    12781 atggcctcga tgccggtgcc gacgattatc tgatcaagcc gttcgccatc gaggagctcg
    12841 ccgcgcgcgt ccgtgcgctg ctgcgccggc cgggcgcgcg ttcgagcgcg ctccttgccg
    12901 tggggccgat ccagttcgac acgatcgccc ggacggcgac gtgcagcggc gagccgctgc
    12961 atctcagtca gcgcgaggcg gcgctgctcg agttgatgat ggtcaaggcg ggcagcgttg
    13021 tcagccgcga tcagatagag gcgtcgctct atcctttcga cgatccggtg acgcccaacg
    13081 cgatcgaggt gctggtatcg cgggtcagga aaaagctggc gacgctgggg gtgaacgacc
    13141 agctcgtaac gctccgcggc gtcggctacc tgatgatcga gcctgtcggc ggatgaccgg
    13201 cggacgatcg atcatcgcgg agctgcgatc gggtttcgcg tggatggcag cggcgggcag
    13261 tatcgtactc gccgcttttc tcgtcgtcga tttcgaactg ctccaggccg aaatccgtgc
    13321 aacatacgga cgtatcggta tcgcgcacga ggtcgtcgac catgtgatat tcccgatcat
    13381 cgtcctcctc gccccgctgg cgatcgcgac ccggtggttg attggccgcg cgctgcgccc
    13441 gctgcacgac gccgccgacc tcattcacca ggcaacgggc cgggaacggg gttttcgcgt
    13501 taggcttgcc gattttccga ccgaagctct gcctttcgcc gaagcggtca attcactgct
    13561 ttcgcggctc gacgatgcgg cgcggcggca ggaagggttc gccgccgatg tcgcgcatga
    13621 gctcaagacg ccgctggcgg tcgcgatgct cgaactcgaa cggctcaaga ccgccgatac
    13681 cgaccgggtg atcgccgatc tggcggcgat gaacaggctg gtcgagcaat tgctcatact
    13741 cgcacagctc gatgcctatg cggccgcgcc gcagcccttt cggcccatcg accttcggga
    13801 tccggcgctg gaggcgatca agctttgtgc ggtcgacgcg gcgcagaagg gcgtggcgct
    13861 agcataccgg gacgaaggcg ccgcggcgcc ggtcatgggt cgcatcgaag cggtggccgc
    13921 ggcgctgcgc aatctggtcg aaaatgccat ccgggtaacc gccgcgggcg gagaggtggt
    13981 gatcgttcgc gggccggggc caagcatccg ggtcgccgat ggcggggccg ggatcgaccc
    14041 ggacaggctg ctgaccctgt ccgatcgttt ccggcgcggc gaacatgcca gtttcggcgg
    14101 cgctggcctt ggcctgtcga tcgtcgcacg gatcatggaa gttcatggcg gaacaatgcg
    14161 ttcgcatccc gacgagccgt cgatcgagct ggatttcggg gcaaatgtag catcctgaga
    14221 caagtttggg cctcgtaagc tttctgtcag caaaatccgc taggatagga gcgaaggatt
    14281 cggtgggggc cgaacctcgc gacggattgg ctcaagaaaa gataaggtga gtggctatcc
    14341 ggcgttcaca gcgggtcgca accgttgatg gactgtgggg gaaatatctt gtcggctgta
    14401 ttgacggcgc tggtgtggga acggcaattg ctttgccgga gcgggctcgc ttccattgtt
    14461 cggcgcgaat tcgcaaacat tgaggtcgcg accgccggcg acgccgatca ggcgttggcc
    14521 tggctggcgg cgaccgatgc gcccggcttg cttattctcg acggcggaat cgacctggca
    14581 tccgtggtgc cctatggcat tcgcggattg cgtcggcgtt acccccggct cgcactggtg
    14641 atcgtcgact ggcgacgcga tcgagacgcc gtgctcggtg ccatcggcga aggggcgcac
    14701 ggctttatcc agaaggatat ggagcaggat gaaatgctcc aggcctttcg gcaggtgctc
    14761 gcggggcagg tttatgtccc gccgatcgtc agcgacgttg atacggacat gccggtcgct
    14821 ggctcttccg gttcgcagca ggtatcgatc accagcctga ccgaacggca gcgcgaagtc
    14881 ctgacccaca tgtcgatggg caaatcgaac aaggaaatcg cccgctcgct ccgtatctcc
    14941 gaaagcacgg tcaaagtgca tgtggcggct gccttccgcc tcctcggcgt tcgcaaccgc
    15001 gtcggggccg tggcggtgat ccagggccgg agcggcgcat cggcgttcat gcgcgaccat
    15061 ctgctcggcg cacgccgggc gtacgagccg cgcaaacacg ccttcggttg aaatatcgga
    15121 aagctaatca agctgcacga tttcggcgtg ctagttaatc gcgctgcaat catggccgaa
    15181 agtattagat agtcttgcca gatcgataat accgaggact catggagtcc cgttttgggc
    15241 taataccgaa agcccaatcg actccggcgc ctagattttt ttaagctttc ggtaagtcgt
    15301 cgggcgagtc cattgactcg tgacgttcgg cgctgctggc ggggccgcac cagctctatg
    15361 ttcaaccttt cgtccgcacg aattttgcgg atgaggccgc tcgcgcctgg ttcgcgggac
    15421 ggcaggaccg gagacgtgac atggtaaagc tgactcaaca tgacctcaat ttcatccttc
    15481 agcagatcga gatcgccgag gagcataacc ggctggtgca ggaagacggg atggatccgg
    15541 gtgaggcgct gcgcctgctc gtcgacaatc cgctccttcc caacggattg cgcaccgtcg
    15601 acggtagctt caacaatctt gttccgggcc gcgagctttg gggtgcgtcg ggcgaggcct
    15661 ttccccggct gacgacgccg aactgggtca acgaaagcga cgattcgatc atcttcggcg
    15721 cgggatcgcc ggggcagatc agttttaccg acggcaatta tggcgaaaac gggccgcctt
    15781 cagggcccgg cggccttggc ggcgggacgc tggtcgatgc tgatccgcgc accatttcca
    15841 acctgatcgt cgaccagaca ctgaacaatc cggcggtcat tgcagcggcg ctccgcctcg
    15901 cggggcacgc cgaaagcgag atcatggacg gcatcgcagc catccggacg gcgatgcagg
    15961 cgcaccctga cgcgctggag gcactggcgg gagctaatct ggcgctgacc gacgcccagg
    16021 cggcctataa tcaggcggtc gccaacctga tcgccgaaac cgatccagcc gccattcccg
    16081 ccctcatcac ggccgtctcc actgccgcca cggcgcaggc cgaggcgcag gtcgccgtca
    16141 caaccgcaga agatgcggtc gaggtcgccg aaggcgtgct ttcggcgacc ctcgacgact
    16201 atggcgtcga gatggacggc aatagcgtgc tcctgcgcaa cgtgtcgccc gatgagggcc
    16261 tgtcggcatc ctacaacggc tggtttaccc tctttggcca gttcttcgac catggcctcg
    16321 atctcgtctc caagggcggc aatggcacgg tctatatccc gctccaaccc gacgatccgc
    16381 tttataaccc cgacagcccg cacaccaatt ttatggcgat gacgcgcgcc acggtgggcg
    16441 taggcgcggg caacgtcacg acgccctggg tcgaccagaa ccagacctat acgtcgaatg
    16501 cctcgcacca gctattcctg cgcgaatatg aaatggtggg cggcaagccc gtttcgaccg
    16561 ggcatctgct cgagggcgat cgcggcatgg caacctgggc cgacgtcaag gaacaggcgc
    16621 gcacgatgct cggcatcgaa ctgaccgacg ccgacgtcac gcaggtgccg gtcttccgga
    16681 tggatcccta tggcgagttt atccgcggcc cgaacggctt gccgcagatt ctcgccgctt
    16741 tcacccctga gggcgagccc atctatgtcg agggcaacct cgcgagcccg atcaacccgt
    16801 cggcgatcca gcttcccgtc ggcaccgtga tcatggggtc gggcggcgac aatgtgattg
    16861 aggcgggcga aaccgtttcg gcggcgcgca ccggcaatgc cttcctcgac gacatcgccc
    16921 atgccgcggt gccggtcgtc gccggcggcg tgctgcaaca ggatgccgac gatgccgtcg
    16981 gctattcggg cggcttcaac acgcgcggtc agcagacgca atatgacgac gaaatgctcg
    17041 acgcgcacta catcacgggc gacgggcgcg ggaacgagaa tatcggtctg accgcggtcc
    17101 accacgtctt ccactccgaa cacaaccggc aggtcgacga gatcaagaag acggcgttcg
    17161 aatcgggcga cctcgccttc ctcaaccagt ggctcgccac tgatgtgacg agcattcccg
    17221 cgacgatgga cgggctggtg tgggacggcg aacgcctgtt ccaggcggcg cgtttctcga
    17281 acgaaatgca gtatcagcac ctcgtgttcg aagagttcgc gcgcaagatt cagcccgacg
    17341 tcgatatctt cctcgtcgaa cctgatgtcg agctcaatcc ggcgatcttc gccgagttcg
    17401 cgaatgtcgt ctatcgcttc ggtcattcga tgctgaacca gacggtcgat cagcagaatg
    17461 cggacggcac cgtcaccaac atggaattgt tcgacgcctt cctcaatccg ctggcgttcg
    17521 gatcggatac ggtcgatcac cgcgaggccg cggcggcgat tgtgcgcggc atgacgatgc
    17581 aggtcggcaa cgagatcgac gaattcgtca ccgacgtgct gcgcaaccag ctcgtcggca
    17641 ttccgctcga cctcgccgcg ctcaatatcg cgcgcggccg cgacaccggc atgccgacgc
    17701 tcaatcaggc gcgcgcgcaa ttccaggaca tggcgaccgg cgacacgctg ctgcgcccct
    17761 acaccagctg gaccgacttc gcgctcaaca tgcgcaaccc cgaatcggtg gtcaatttca
    17821 tcgcggccta tggcacgcat gcgacgatca cggccgaaac gacgatagag ggcaagcgtg
    17881 acgccgcgat ggcgctcgtc ttcggcggcg agggggcgcc aggcgaccgg ctcgacttcc
    17941 tcaacgcgac cggagcctat gcgggcggca gccttggtgg gctcaacgac gtcgatctgt
    18001 ggatcggggg gcttgccgaa aagaagatgg cgttcggcgg catgctggga tcgaccttct
    18061 ccttcgtgtt ccagatgcag atggaaaatc tgcaggactc ggatcgcttc tattatctgt
    18121 cgcgcacgca gggtctcaac ctgctgactg agctggaaaa caactcgctt gcgaagatgg
    18181 tcaagaagaa caccgatctc gacggcttcg cgttgccggc ggacatcttc tcgacccccg
    18241 atcacatcct ttacatggat tataacaagc agttggcgat gacgaacacc gccgatccgg
    18301 tgcacgaaaa tgccgtgctg cagggcatct ccagcctcgt cgagcgccgc gatgtcgatg
    18361 gcaacggggt ggcggaatat atccgctaca acgggctcga ccatatcgtc atccagggca
    18421 ccagcgccaa cgaccatatc gtcgcgggcg gcggtgatga caccgtctgg ggcgaggacg
    18481 gcgacgaccg tatcgaggcc ggctacggtg tcgacagcat caacggcggc gcgggcgacg
    18541 acatcatcac cagtgcggga accgacatcg gtgccgtttc ggtgctgaag ggcgaaaccg
    18601 gcaacgacgt catcgtcgac ggcaccggca tgtcgctgat cttcggcggc gacgggaagg
    18661 attatatcgt ctcgggcttc gacgacggcg aaatccgcgg cggccagggc ggcgatttca
    18721 tctatggcgg cgacggcatg ggcatgctct tcggcaacga gggcaatgac tgggtcgaag
    18781 gcggcggcgg tttcgacttc atcgccggcg acatggccga actcttcttc aactcgacga
    18841 tcatcggcca cgacgtgctc aacggcgggc agggcgatac cgattatgac gcggattcgg
    18901 gcgacgacat catgttcggg ggcgagggca tccagaagaa tatcggcatg tggggccacg
    18961 actgggtgac ccatcaagga cagtcgagcg gcgtcgacgc cgacatgcgc gtcgacatct
    19021 tcacgacgtt gcccaacgag gtgctgcgcg atcgcttcag ccaggtcgag gcgctgtcgg
    19081 gctggaatgg caacgacgtg ctgcgcggcg acgaccgcac cggtgccgat gccgagggcg
    19141 gcacgacgac cgacccgacg cccgaaacgg gctttcgcta caatgagctc aaccaggcgg
    19201 ggatggaccg gatcgccgga ttgtcggaga tcatcacgcc cgacctgatg cagacgctgc
    19261 cctattgggc aaccggatcg tgggaggacg gcgcgaccgg gacgaccgag gcggtcttcg
    19321 tcggcggcaa tatcctgctc ggcggcgggg gcagcgacac gatcgagggc cgcggcggcg
    19381 acgatgtgat cgacggcgac cgctatctca acgtcaagct gaccggcttt accaaggacg
    19441 gcatcgcatt cagcgtcacc agcatggaag attcggtgac gctgggcggc gtcaccaagt
    19501 cgctgacgag ctggatgatc gacggccgga tcaacccggg tacgatggag atcgtccgcg
    19561 aaatcctgtc cgatgacagc ggcgaggaca ccgccgtctt ctgggacgtg ctcgaaaatt
    19621 acgcgatcac gagcaacggc aacggcgggc tgcggatcga acatgtcgac cagaccggcg
    19681 gggcgatcga ccccgtcacc ggccgcaacc gcgaagccga cggcatcgac caagtctata
    19741 atgtcgagtg gctgcagttc ggcgatcaac ggatttcgac cgcggggctg atcaacacgc
    19801 cggcgaccgg tgcgccggtg atcagcgacc ttacgccgac caaaggtcag gcgctgacgg
    19861 cgaatgtcgg ctcgatccag gacgaaaacg gcctcggcac cttcggctat cagtggcagg
    19921 tttcgagcga caatggggcg acctggaata acgtcgttac tggcgggacc ggggcctcct
    19981 ttaccccgac cggcggtcag gtcggcggtc tgatgcgcgt cagcgtcagt ttcaccgacg
    20041 gtgcagggca tcaggagacg ctggtttccg caccgaccgg tgtcgtcggc gacaattggg
    20101 acgcgattcc cttcctcgcc aacacgttca acggcaccgc cggcgatgat atcgccgacg
    20161 gaacgaacgg cctgttcgga gcgggtgccg ccgatacgct caatggcaac ggcggcaacg
    20221 acatattgag cggtgcgggc ggaaacgaca cgctgaacgg cggggcgggc gatgatcggc
    20281 tcgacggcgg cgcaggaaat gacgtcgcgg tctttgccgg aaatgttgcc aatttcgcac
    20341 tggcgtcgaa tggcaccaat ctggtcgtga ccgacctgac gggtgccgaa ggcaccgaca
    20401 cgctgagcac gatcgaaacg cttcgcttcg ccggcaccaa ctatagcgtg gtggcgggaa
    20461 cgggggcgaa caacgccaac ctcaacggcg cgtctggtgc ggccggatcg caggcggtgt
    20521 tcggctttgc cggcaccgac atgctcaacg gcggcggggg taacgacttg ctggtcggcg
    20581 gccagggcaa cgacacggtg aacggcgggg ccggcaacga taccgcctat tggtcggtcg
    20641 gcgacggccg cgacttcgtc aatggcggcg ccgataccga cacggtcaag gtcgtgggcg
    20701 atgcgagcgc cgaaaccttc cgcatttatt cgcgcgccgc ggcgctggcg gcagggctga
    20761 ccgggctcaa cgccaatgcc gagatcatca tcacgcgcaa cggcaccacc aacgccgaca
    20821 tcatcgccga actcgacaat gtcgaggaga tcgtgatcaa tggccgcggc ggcggcgaca
    20881 cgttcctccc gatcggtgac ttcgctccaa ccagcctgtc gaccagcacg atcacgatcg
    20941 agggtagcga cggcgacgat gtcgtcgaca tcggcgcgct ccagtcggcc caccgcgtcg
    21001 tgttcaaatc gagcggcggc gacgacgtca tcctgggcca gatgcgcgat caggatcgct
    21061 tcgaactcgc cgacggcaag accatcgacg attatacggc gaccgaaaat gccgacggta
    21121 gcacgacact gtcgaccgaa gggcacagcg tgaccttctt tggcaccacc gcaaatgcgg
    21181 gaggcgcggc ggcttcggat gatgaagatc atgggcatga caacgaccat gacgacgacg
    21241 atacgccgcc gccctctgac gccgatacgc tgaccggaac cgccgggcgc gacgtcctga
    21301 acggcggctc gggcgacgac gagcttgaag ggcgcggcgg tgacgacacg ctctcgggcg
    21361 ggtcgggtga cgacgtgctg aaaggcggcg gcggcgacga catgctctcg ggcgggtcgg
    21421 gcgacgatgc gttatcgggc ggcgcggg
//
LOCUS       DATMSU010000109         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_125506,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000109 DATMSU010000000
VERSION     DATMSU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125506"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..2193)
                     /locus_tag="VN034_02930"
     CDS             complement(79..2193)
                     /locus_tag="VN034_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWV59581.1"
                     /translation="MIRASLAALALSCSLAAVAAAAPQDDDPYLWLEDIEGAKALDWV
                     KKENAATDKLITSRPGFEADRKRARAILDDERQIAMPGEVMGETITNFWRDADNPRGI
                     WRQSPLDAYLAGKPVWTTLIDVDALGKAEKQSWVWHGADCLAPDYKRCLVSLSPGGTD
                     ADIVREWDRTTKSFVEGGFTLPQAKSSVTWEDADTLLVATDYGEGSMTASGYPRIVKR
                     WKRGTPLSAAETLAEGEHEDVGMNVFALVDGDKRWPMISRGKTFYTADYMLPNIDASR
                     YISTIIPDTASIRDIVDGQLVIFLNQDWANIPAGSLVSLSIEDMSADRKVPIVTAMTP
                     TKAQAIEDVAATDNILWVKALDDVEGKLFALRRDPATGKWSQKDVPLADNATVSIAGT
                     VGKRDIVLATAETMLTPPTLYAAAETGAPKAVQSLPATFDAKDMSVEKRFATSKDGTK
                     IPYFLVRKTSDSEASVPALIHAYGGFRAAQTPGYLTGQPYRAGPLGLFWVEDGNAYVL
                     ANIRGGGEYGPAWHQAALREKRQNSFDDLHAVAEDLVKTGVSAKGKIAISGRSNGGVL
                     VGAAMTQRAELYGAVISGSPIKDMWRYDKMLAGASWVAEYGDPDVPEDWAFLSKYSPY
                     HNIRKGVTYPPIFLYLSTKDDRVHPGHARKFAARLMENGNRVYYHEYIEGGHSVGADH
                     AEDAVRAAMLHGFLLRELVEKK"
BASE COUNT          363 a          727 c          777 g          448 t
ORIGIN      
        1 gacgcaccgg cgagatcccg gccttcgccg ggatgacgga agagaggtgg tggcgcgcgt
       61 ccgggggcgg tctagcgcct acttcttttc caccagttca cgcagcagaa aaccgtggag
      121 catcgcagcg cgtaccgcat cctcggcatg gtcggcgccg accgaatggc cgccctcgat
      181 atattcgtga taatagacgc ggttgccatt ctccatcagc cgcgcggcga atttgcgcgc
      241 gtggccgggg tggacgcggt cgtccttggt cgagaggtag aggaagatcg gcggataggt
      301 cacgcccttg cggatgttgt gatagggcga atattttgac aggaaggccc agtcctcggg
      361 cacgtcggga tcgccatatt cggcgaccca tgaggcgccg gcgagcatct tgtcatagcg
      421 ccacatatcc ttgatcggcg agcccgagat caccgcgccg taaagctcgg cgcgctgcgt
      481 catcgccgcg ccgacgagca cgccgccgtt cgagcggccc gagatcgcga tcttgccctt
      541 ggcgctgacc ccggtcttga ccagatcctc ggcgaccgcg tgcagatcgt cgaagctgtt
      601 ctggcgcttt tcgcggagcg ccgcctgatg ccaggcggga ccatattcgc cgccgccgcg
      661 gatattggcg agcacatagg cgttgccgtc ctcgacccag aacagcccga gcgggccggc
      721 gcgatagggc tggccggtca gatagcccgg cgtctgcgcg gcgcggaagc cgccataggc
      781 atggatcaac gcggggacgg acgcttcgct gtccgatgtc ttgcggacga ggaaataggg
      841 gattttggtg ccgtccttcg acgtcgcgaa ccgcttttcg acgctcatat ccttcgcatc
      901 gaaggtcgcg ggcaggctct gcaccgcttt cggcgcgccg gtttcggcgg cggcgtagag
      961 cgtcggcggc gtcagcatgg tctcggcggt cgcgagcacg atgtcgcgct tgccgaccgt
     1021 tccggcgatg ctcaccgtgg cgttgtcggc gagcggcaca tccttctggc tccattttcc
     1081 ggtcgcgggg tcgcggcgca gcgcgaagag cttgccctcg acatcgtcga gcgccttgac
     1141 ccacagaata ttgtcggtcg cggcgacatc ttcgatcgcc tgcgccttcg tcggcgtcat
     1201 cgcggtgacg atcggcacct tgcggtcggc cgacatatcc tcgatcgaga gcgaaacgag
     1261 tgagcccgcc gggatgttcg cccagtcctg attgaggaag atgacgagtt gtccgtcgac
     1321 gatgtcgcgg atgctcgcgg tatcggggat gatcgtcgaa atataacgcg aggcgtcgat
     1381 gttcggcagc atatagtcgg cggtgtaaaa ggtcttgccg cggctgatca tcggccagcg
     1441 cttgtcgccg tcgacgagcg cgaagacgtt catcccgaca tcctcatgct cgccctcggc
     1501 cagcgtttcg gcggccgaaa gcggcgttcc gcgcttccac cgcttgacga tgcgcggata
     1561 acccgacgcg gtcatgctgc cctcgccata gtcggtcgcg acgagcaatg tgtcggcgtc
     1621 ttcccacgtc acgctgcttt tcgcctgcgg cagggtgaag ccgccctcga cgaagctttt
     1681 ggtcgtgcga tcccattcgc ggacgatgtc ggcgtcggtg ccgccggggc tcagcgagac
     1741 gaggcagcgc ttatagtcgg gcgcgaggca gtcggcgccg tgccagaccc agctctgctt
     1801 ttcggccttg ccgagcgcat cgacgtcgat cagcgtcgtc catacgggct tgccggcgag
     1861 ataggcgtcg agtgggctct ggcgccagat tccgcgcgga ttgtccgcgt cgcgccagaa
     1921 gttggtgatc gtctctccca tcacctcgcc cggcatcgcg atctggcgtt cgtcgtcgag
     1981 aatcgcgcgc gcgcgcttgc gatcggcctc gaaaccggga cggctggtga tcagcttgtc
     2041 ggtcgccgca ttttcctttt tgacccaatc gagcgccttc gcgccctcga tatcctcgag
     2101 ccagagatag gggtcgtcgt cttgcggcgc ggcggctgcg accgcggcga gggaacagct
     2161 gagggcaagc gcggcgaggc tggcgcggat cattcgatta tctcccctgg ggtgtggtgc
     2221 cgctgtgcta gcagcattac acacccctag aaagcatatc gtcatcccgg cgaaggccgg
     2281 gatcttgccg ttccgtcagg ccgaggtggc gagat
//
LOCUS       DATMSU010000110         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_68446, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000110 DATMSU010000000
VERSION     DATMSU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68446"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..2389
                     /locus_tag="VN034_02935"
     CDS             161..2389
                     /locus_tag="VN034_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59582.1"
                     /translation="MRNLSVLLMAAAAMPTCAWAQNTVPAGEAATDSSGDIIVTANKR
                     AERQLDVPSAITAFRGSDLLDDGYTSLKDYLALTPGVQVSQAGGGGTPVIRGLTTGTN
                     LGAIVGIVIDGAPVGPSSSFNLGGANSTDLDPIDLERVEVLKGPQGTLYGANTLGGLV
                     SYTFARPDLSEVTAIARGELSGTENGGTNYSIRAAVSAPLVRDKLGARISGYIDRPGG
                     FVDNDVAGINDFNRQRNWGINGSLRFEPTADLSINIVGFHQRVNQAGQDYVIYNANGQ
                     PLSDLHYDQQLYNKYRKTSDVLIGTVDYDLGFGSLTSVTSYQKIDSYIVLNALTFTAQ
                     LPFFTLFGGAAVPPGTAAGADAKGPIKKFSQELRLASSGDGPLQYVVGAFYTHEKART
                     STNVVGFDADFNEVPTLDPALGFGVNDTYKEIASFGNLTYKFSDTFDLTGGLRIGRID
                     QSYNQTFYGSDSAAFNTFLVLQGLTPVPADTGTTRGAETVKTYLLTARYHFSPDGMIY
                     ARYATGFRPGGPNFNVPGLPASFDADSTENFEGGVKTKFWDGKGTIDVSGFYMRWKDI
                     LIQVSSGGLNAYTNGGNARVYGFEGSLSLQPVEGLTLAGTLSYNDRKITSIDPLAAAS
                     LGVGDPLPNSPKWSGSLALDYRTPVAGEWQAVVGATAKFVGQRHATLRSSILQPDYLI
                     PKFALFDLRAGVESDHVDISLFVRNLTDKRAQLSATTANGINEVVVQRPRTIGASVTF
                     KY"
     gene            2518..3228
                     /locus_tag="VN034_02940"
     CDS             2518..3228
                     /locus_tag="VN034_02940"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWV59583.1"
                     /translation="MREKTGRKQELATSRLRVGAAIRERRLAAGLSLAELADRIGVAL
                     STMSKIENGKISTSFERLDSVSRALQADITAFLGTAAPMAIAMQQSATYGTRRSITRP
                     EDGKMVDAGSYLEWFHASDMLQKRFQPVVAELLIEDVAQYGAFTQHSGEEFNYVLEGE
                     MEFHTEIYAPVRLTAGSSIYFDAEMKHAHVRVGSGPCRILAILCPRMEPLGTPAPDRR
                     SMRVVDAHGTSLAPVTAK"
     gene            3239..4483
                     /locus_tag="VN034_02945"
     CDS             3239..4483
                     /locus_tag="VN034_02945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWV59584.1"
                     /translation="MLRESEVPTAMTALSKAHNIADLRALAARALPRPIREYLEGGAD
                     DEWTLARNRAAFQDWVLAQHTLVDVSAIDPGTDLLGFQSAMPLMLSPTGMSQLFHASG
                     ELAVARAAAQAGVPYGLSTMATTSIEAIAATGAKRYFQLYLFRDRGLTRALLDRAAAH
                     GYGALCLTVDTAMAGNRERDLRSGMIMPPRFTPGSLLSFATHPRWSMGALKNRSFRLA
                     NVVEHVGDIGREGTSVIDYVNAQFDRSATWQDVEWLRAQWPGKLVVKGAIMPDDCEEA
                     ARRGADAIMVSNHGGRQLDGVAAPLDHLPAIRDRVQDRAQLIVDGGVRRGTDILKAVA
                     LGADGCSIGRPYLYGLAAGGEAGVARVLSIFRSEIERDMGLMGRTRIADITPDDVDHL
                     SKFRAKPGERRADIFRKAGLQR"
     gene            4480..>4948
                     /locus_tag="VN034_02950"
     CDS             4480..>4948
                     /locus_tag="VN034_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HWV59585.1"
                     /translation="MTSWSSVEIKAVTDEAPTVAFSDADVIFAPDALRSRVASAVEAT
                     GFDAKPGRWLDLIGEGGDRIVVVAVGAKDSADRWRNAGGHLVDAIRALTLKALRLPAA
                     SDLGIGDALADIIEGALLHGFRLDQGRRDPAPGQVDAALYIAEDDLALADRAQD"
BASE COUNT          887 a         1576 c         1609 g          876 t
ORIGIN      
        1 tgggtattct ggagatcatt agcgcatttt cctcatgatt atgttcctgc ctgaatggcc
       61 tccggaattg atggattgac agtttcgtga aaatgagttt ccaataagga aagatagaaa
      121 gcgtccaatg gcgacgccaa cagtggggat ggatatgacc atgcggaacc tgagcgtgct
      181 cctgatggct gcggccgcga tgcctacctg cgcatgggcg caaaacaccg ttcccgccgg
      241 ggaagcggca accgacagca gcggcgatat catcgtgacg gcgaacaagc gcgccgagcg
      301 ccagctcgac gtgcccagcg cgatcaccgc ctttcgcggc agcgatctgc tcgacgacgg
      361 ctataccagc ctcaaggatt atcttgcgct gacccccggc gttcaggtca gccaggccgg
      421 cggcggcggc acaccggtca ttcgcgggct gacgacgggc accaaccttg gcgcgatcgt
      481 cgggatcgtg atcgacggcg cgccggtcgg acccagctcg tcgttcaatc tcggcggcgc
      541 caactcgacc gatctcgacc cgatcgacct cgaacgcgtc gaagtgctca aggggccgca
      601 ggggacgctt tacggcgcca acacgctcgg cggccttgtg tcctacacgt tcgcgcggcc
      661 cgatctttcg gaggtcaccg ccattgcgcg cggcgaactc agcgggaccg aaaacggcgg
      721 caccaactat tcgatccgcg ccgcggtcag cgcgccgctt gtcagggaca agctcggcgc
      781 gcgtatttcg ggctatatcg accggcccgg cggcttcgtc gataacgacg ttgcggggat
      841 caacgatttc aaccgccagc gcaattgggg catcaacggt tcgttgcgct tcgaacccac
      901 cgccgacctc agcatcaaca tcgtcggctt tcaccagcgc gtgaaccagg cggggcagga
      961 ctatgtcatc tacaatgcga acggccagcc gctgagcgac ctccattacg accagcaact
     1021 ttacaacaaa taccgcaaga cgtccgacgt gctgatcggg acggtcgatt atgatctggg
     1081 gttcggcagc ctgacctcgg tcaccagcta tcagaagatc gatagctaca tcgtcctcaa
     1141 cgcgctgacc tttacggccc aactgccctt cttcacgctg ttcggcggcg ccgcggtccc
     1201 gcccggcacg gctgcgggtg ccgatgcgaa ggggccgatc aagaaattct cgcaggaact
     1261 gcgcctcgcg tcgtcgggcg acggaccgct gcaatatgtc gtcggcgctt tctacacgca
     1321 cgaaaaggcg cggacatcga cgaatgtcgt gggcttcgat gctgacttca atgaagtccc
     1381 gacgctcgat ccggcgctcg gcttcggcgt caatgacacg tacaaggaaa tcgcgagctt
     1441 cggcaatctg acctacaagt tcagcgatac cttcgacctg accggcggcc tgcgtatcgg
     1501 gcggatcgat cagtcgtata accagacctt ctacggcagc gattcggcgg ccttcaacac
     1561 cttcctcgtc ctgcagggtc tgacgcccgt acccgccgat acggggacga cgcgcggtgc
     1621 cgagacggtg aagacttacc tgctcaccgc gcgttatcac ttctcgcccg acgggatgat
     1681 ctatgcacgc tatgcgaccg gcttccgtcc cggcggcccg aacttcaacg tgccgggttt
     1741 gccggcgagc ttcgatgcgg actcgaccga gaatttcgaa ggcggggtga agaccaaatt
     1801 ctgggatggc aagggcacga tcgatgtgtc gggcttttac atgcgctgga aggatattct
     1861 catccaggtc agcagcggcg ggctcaatgc ctacaccaat ggcggcaacg cccgcgtcta
     1921 cggcttcgag ggatcgctgt cgcttcagcc ggtcgaaggc ctgacgctgg ccggcacgct
     1981 ttcgtataat gaccgcaaga tcacgagcat cgatccgctc gccgccgcgt cgctcggcgt
     2041 gggcgacccg ctgccgaaca gccccaaatg gagcggttcg ctcgcgctcg actatcgcac
     2101 ccccgtcgcg ggcgaatggc aggcggtcgt cggcgcgacg gcgaaattcg tcgggcagcg
     2161 ccacgcgacg ctgcgctcaa gcatattgca gcccgactat ctgatcccga aattcgcgct
     2221 cttcgatctg cgcgccgggg tcgaaagcga ccatgtcgac atcagtctgt tcgtccgcaa
     2281 cctgaccgac aagcgcgcgc aattgagcgc gaccaccgcc aacggcatca acgaggtcgt
     2341 ggtgcagcgt ccgcggacga tcggcgcctc ggtcacgttc aaatattgat cgaaacgcca
     2401 gtttgatgtc gctcggcgcg ttctttcatg caaaagcccg gcctttcttc ttgcgcgggc
     2461 gggctgtcac cggccatgtc gatctcgcgc gggcaggaca gggattttcg aaacaagatg
     2521 cgcgagaaga cgggacgcaa acaggaattg gcgaccagcc ggctgcgggt cggcgcggcg
     2581 atccgcgagc ggcggctggc ggcgggcttg tcgctggccg aactcgccga ccgcatcggc
     2641 gtcgcgctgt cgacgatgtc aaagatcgag aatggcaaga tctcgaccag cttcgagcgg
     2701 ctcgacagcg tcagccgagc gctgcaggcg gatatcaccg cgtttctcgg caccgcggcg
     2761 ccaatggcga tcgcgatgca gcagtcggcg acttacggga cgcggcgcag catcacgcgg
     2821 cccgaggacg ggaagatggt cgacgcggga tcctatctcg aatggttcca cgcctcggac
     2881 atgctgcaga aacggttcca gccggtggtc gccgaattgc tcatcgagga tgtcgcccaa
     2941 tatggcgcct tcacgcagca cagcggcgag gaattcaact atgtcctcga gggcgagatg
     3001 gagtttcaca ccgaaattta tgctccggtt cgtttgaccg cgggatcgag catctatttc
     3061 gacgccgaga tgaagcacgc gcatgtccgg gtcggcagcg gtccttgccg catcctcgcg
     3121 attctctgtc cgcggatgga gccgctgggt acgcccgcgc cagaccgtcg gtcgatgcgc
     3181 gtcgtcgacg cgcatgggac gagtttggca cccgtgacgg cgaaatagca gcggatcgtt
     3241 gcttcgcgag tcggaggtgc cgaccgcgat gaccgcgctc tcgaaagccc ataacattgc
     3301 cgacctgcgg gcgctcgccg cccgcgcatt gccgcggcca atccgcgaat atctggaggg
     3361 cggcgccgac gacgaatgga cgctggcgcg caaccgcgct gcctttcagg attgggtgct
     3421 cgcgcagcac acgctcgtcg acgtcagcgc gatcgacccc ggcaccgacc tgctcggttt
     3481 tcaaagcgcg atgccgctga tgctgtcgcc gaccggcatg tcgcagctat tccatgcgtc
     3541 gggcgagctg gcggtcgcgc gcgcggcggc gcaggcgggc gtgccctatg gcctgtcgac
     3601 gatggcgacc acctcgatcg aggcgatcgc cgctacgggc gcgaagcgct atttccagct
     3661 ctatctcttt cgcgatcgcg gactgacgcg cgcgttgctc gaccgcgcgg cggcgcatgg
     3721 ctatggcgca ctctgcctga ccgtcgacac ggcgatggcg ggcaaccgcg agcgcgattt
     3781 gcgcagcgga atgatcatgc cgccccgctt cacgcccggc agcctgctga gcttcgccac
     3841 gcatccgcgc tggtcgatgg gagcgctcaa aaaccggtcg ttccggctcg ccaatgtcgt
     3901 cgagcatgtc ggcgacatcg gccgcgaggg gacgagcgtc atcgattatg tgaatgcgca
     3961 gttcgaccgc tcggcgactt ggcaggacgt cgaatggctc cgcgcgcaat ggcccggaaa
     4021 gctggtcgtc aagggcgcga tcatgccgga cgattgcgaa gaagccgccc ggcgcggcgc
     4081 cgatgcgatc atggtgtcga accatggcgg gcgccagctg gacggggtcg cggcgccgct
     4141 cgaccatctc cccgccattc gcgatcgcgt tcaggatcgc gcgcagctga tcgtcgacgg
     4201 cggcgtcaga cgcggcaccg acattttgaa agcggtcgcg ctcggcgccg acggttgttc
     4261 gatcggccgg ccctatctct atggccttgc ggcgggcggc gaggctggcg tcgcgcgcgt
     4321 tctatcgatc ttccggagcg agatcgagcg cgacatgggg ctgatggggc ggacgcggat
     4381 cgccgatatc accccggacg atgtcgacca tctgtccaaa tttcgagcga agccgggcga
     4441 gcgcagggct gatattttcc gcaaggcagg attgcagcga tgacgagttg gagcagtgtc
     4501 gagatcaagg ccgtcacgga tgaggcgccg acggtggcgt tttcggacgc tgatgtgata
     4561 ttcgcccccg atgcgcttcg cagccgcgtc gcttcggctg tcgaggcgac gggcttcgat
     4621 gccaagccgg ggcgctggct cgatctgata ggcgagggcg gcgaccggat cgtcgtcgtg
     4681 gccgtgggcg caaaggacag cgcggaccgc tggcgcaacg ccggcgggca cttggtcgac
     4741 gccatccgcg cgttgacctt gaaagccttg cgccttccgg cggcgtcgga cctcggcatc
     4801 ggcgatgcgc ttgccgacat tatcgaggga gccttgctgc atggattccg gctcgaccag
     4861 gggcggcgcg acccggcgcc cggccaggtc gacgcggcgc tctatatcgc cgaggatgat
     4921 ttggcgctcg ccgaccgcgc gcaggatc
//
LOCUS       DATMSU010000111         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_49378, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000111 DATMSU010000000
VERSION     DATMSU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49378"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2759
                     /locus_tag="VN034_02955"
     CDS             <1..2759
                     /locus_tag="VN034_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59586.1"
                     /translation="QDAADAPAAPAAQSYADAEITVTGSRIQRDGFRAPTPLTVLNSE
                     DIESSAPANIADYVNDIPSLVGSVTPASSNLNISAGTAGVNALNLRALGTARTLVLLD
                     GQRSVGSTMTGLVDVNTFPQGLIKSVEIVTGGASAAYGSDAVSGVVNFILDKDYTGIK
                     GSAEYGITTYGDAPSYRATLTAGTAFAGGRGHLLLNGEIAIKDGLHRVPREWNQQGWY
                     MINNPAYAEGNGAPERLVVSGAGLSLATPGGIITDTALRGTVFGPGGAVGQFDYGTVR
                     DPWMIGGDWESTQVNSFQSLDPAENRKSLFGRLSFEVSDGLNLFVQASYAKSKNLGNL
                     GIQLNQGNVTIQSDNAFLPQSVRDELALRGIDEFKLGTTNANLPVRKNDTRRELQRYV
                     VGANGEFDLFGSSARWDVYYQKGISRTREMARDISNNARLALAQDAVFAPAGNALGVP
                     EGTIVCRSSLTSPGNGCVPFNRIGIGTASQEGLDYVLGNPFRRQKFQQDVFAANLSFD
                     AFELPAGPVSVAIGGEHRREKVSGEVDEEFQTGWFVGNFLPSFGSYNVSEAYLELAVP
                     VFEGFDLNGAIRGTDYSTSGYVTTWKAGLTWEPIPDIRFRATRSRDIRAPNLGELFTA
                     GSTRINVLIDPTQNDASVQFAGTTRGNPLLDPEKADQWGVGVVLQPRFIPGLALSVDY
                     YDIKINGAIGTVAAQTIVDRCAEGVQAFCAAVVRGPNAFGNNLQVFESPFNFAVQRAT
                     GIDFEGSYRLPVGSGDLTLRAMATRYIKNYFDNGIDAPTDTVGQNAPGGTPKWLYRIQ
                     ANWAADGFNFNLTGRGISSGVYDNSFVECTSGCPASTVTNRTINDNHIAGAFYLDTSL
                     TFDVEMAGKELEFYLAVNNLLNKDPAVVAPGPAGSAYATPATNQSLYDLLGRTYRVGI
                     RFKL"
     gene            2774..4348
                     /locus_tag="VN034_02960"
     CDS             2774..4348
                     /locus_tag="VN034_02960"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV59587.1"
                     /translation="MKRLTLAIGALLATTAPALAQHYDVLIRGGTLYDGTGGPARTAD
                     VAIAGDRIAVVGSIPESATATTLIDAAGRIVAPGFIDPHSHAAPNIQTKELAAALPML
                     HQGITTLAINPDGGGPAELGQQIADLNTNIPGVNVIPLIGHNAVRRAVMGDVARLATA
                     DEQAKMEGLVTAAMAAGAYGLSDGPFYIPGKYSNTAEIVGLAKAAAHFPGAFYISHVR
                     DEGNYDIGVVAAVDEVITVAREAKIPGVVTHIKVLGPQVWGKSADVIARIDQARAEGL
                     EIWADQYPYSASGSSLMASLVPGWAQEGGAEALAKRLRDPAMRAKIRAEMVPNLERRA
                     GPHALMIRSFAPDPSLEGKRLDEVGRIKGMAPLDAAIEMLIGGGAPTISFNMSEADVE
                     AFMRQPWTMTSTDGGLPHFGKTSEHPRAYGAFPRKLRNYVLDKPVIPMAQAIHAATGL
                     PAQILGVPDRGLLREGAFADVIVFDPETIGDLATYERPHAYSVGVDYVFVNGKAALAK
                     GKPTAERHGRVLLRPR"
     gene            4421..>4635
                     /locus_tag="VN034_02965"
     CDS             4421..>4635
                     /locus_tag="VN034_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV59588.1"
                     /translation="MNLRHIEIFHAVYTNGSVSAAARALNISQPSVSKTLRHAESILG
                     FPLFERSAGRLVATDDAHALFAEVSEI"
BASE COUNT          820 a         1593 c         1486 g          736 t
ORIGIN      
        1 cgcaggacgc ggccgacgcg cccgccgcgc ctgcggccca aagctatgcc gatgccgaaa
       61 tcaccgtcac cggcagccgc atccagcgcg acgggttccg cgcgccgacc ccgctcaccg
      121 tgctcaattc ggaggatatc gaaagctcgg cgccggcgaa catcgccgac tatgtcaacg
      181 acatcccctc gctcgtcggc agcgtcacgc ccgccagttc caacctcaac atcagcgcgg
      241 gcaccgccgg ggtcaatgcg ctcaacctgc gcgcgctcgg caccgcgcgc acgctcgtgc
      301 tgctcgacgg ccagcgttcg gtcggatcga cgatgaccgg gctcgtcgac gtgaacacct
      361 ttccgcaagg cctgatcaaa agcgtcgaga tcgtcaccgg cggcgcgtcg gcggcctatg
      421 gctccgacgc cgtgtcgggg gtcgtcaact tcatcctcga caaggattat acggggatca
      481 agggatcggc cgaatatggc atcaccacct atggcgacgc gcccagctat cgcgcgacgc
      541 tgaccgcggg caccgccttt gccggcgggc gcggacattt gctgctcaac ggcgagatcg
      601 cgatcaagga cgggctgcac cgcgtgccgc gcgaatggaa ccagcagggc tggtacatga
      661 tcaacaatcc cgcctatgcc gagggcaatg gcgcgcccga acggctcgtt gtgtcgggcg
      721 cggggctcag cctcgcgacg ccgggcggga tcatcaccga taccgcgctg cgcggcaccg
      781 ttttcgggcc cggcggtgcg gtcggccagt tcgactatgg caccgtccgc gacccgtgga
      841 tgatcggcgg cgactgggaa tcgacgcagg tgaacagctt ccagtcgctc gaccccgccg
      901 aaaaccgcaa gagcctgttc gggcgcctca gcttcgaggt ctccgacggg ctcaacctct
      961 tcgtccaggc ctcctatgcc aagtcgaaga atctcgggaa cctcggcatc cagctcaacc
     1021 agggcaatgt cacgatccaa tcggacaacg ccttcctgcc gcagtcggtg cgcgacgagc
     1081 tggcgcttcg gggtatcgac gagttcaagc tcgggaccac caatgccaat ctgccggtcc
     1141 gcaagaatga cacgcggcgc gaactgcaac gctatgtcgt cggcgccaac ggcgagttcg
     1201 atcttttcgg gtcgagcgcg cgctgggacg tctattatca aaaggggatc agccgcaccc
     1261 gcgaaatggc gcgcgacatc agcaacaatg cccgcctcgc gctcgcgcag gacgcggtgt
     1321 tcgcgcccgc aggcaatgca ctcggcgttc ccgagggcac gatcgtctgc cgctcgtcgc
     1381 tcaccagccc cggcaatggc tgcgtgccct tcaaccgcat cggcatcggc accgcgagcc
     1441 aggaagggct cgactatgtg ctcggcaacc cgttccgccg ccagaaattc cagcaggatg
     1501 tcttcgccgc gaacctgtcg ttcgacgcct tcgagctgcc cgccggaccg gtgtcggtcg
     1561 cgatcggcgg cgagcaccgg cgcgagaagg tgtcgggcga ggtggacgaa gaattccaga
     1621 cgggctggtt cgtcggcaat ttcctgccca gcttcggctc ctacaatgtc agcgaggcct
     1681 atctcgaact cgcggtgccg gtgttcgaag gcttcgacct caacggcgcg atccgcggca
     1741 ccgactattc gacctcgggc tatgtcacga cgtggaaggc gggcctgacc tgggaaccca
     1801 ttcccgatat tcgcttccgc gcgacgcgat cgcgcgacat ccgcgcgccc aacctcggcg
     1861 aattgttcac cgccggctcg acgcgcatca acgtgctgat cgacccgacg cagaatgatg
     1921 cgtcggtgca attcgccgga acgacgcgcg gcaacccgct gctcgacccc gaaaaggccg
     1981 accagtgggg cgtcggcgtc gtgctgcaac cgcgcttcat cccgggcctc gcactgtcgg
     2041 tcgactatta tgacatcaag atcaacggcg cgatcggcac cgtcgcggcg caaacgatcg
     2101 tcgaccgctg cgccgaaggc gtgcaggcgt tctgtgcggc ggtcgtgcgc gggcccaatg
     2161 ctttcggcaa taatctgcag gtcttcgaaa gcccgttcaa cttcgcggtc cagcgcgcga
     2221 cggggatcga cttcgaaggc tcctatcgcc tgcccgtcgg cagcggcgac ctgacgctgc
     2281 gcgcgatggc gacgcgctat atcaagaatt atttcgacaa tggcatcgat gcgccgaccg
     2341 acacggtggg gcagaacgcg cctggcggaa cgcccaaatg gctctatcgc atccaggcca
     2401 attgggcggc cgacggcttc aacttcaacc tgacggggcg cggcatttcg agcggggtct
     2461 atgacaacag cttcgtcgaa tgcacctcgg gctgtccggc gtcgacggtc actaaccgca
     2521 cgatcaacga caatcatatc gcgggcgcct tctatctcga cacctcgctg accttcgacg
     2581 tggaaatggc gggcaaggag ctcgaattct atctcgcggt caataatctg ctcaacaagg
     2641 atccggcggt cgtcgcgccg gggccggcgg gcagcgccta tgcgacgccg gcgaccaacc
     2701 agtcgctcta tgacctgctc gggcgcacct atcgcgtcgg catccgcttc aagctgtaag
     2761 gaaaggacgg tcgatgaaac gcctgacgct cgcaatcgga gcattgctcg ccaccaccgc
     2821 gcccgcgctc gcccagcatt atgacgtgct gatccgcggc ggcacgctct atgacggcac
     2881 cggcggtccc gcgcgcaccg ccgacgtcgc catcgcgggc gaccgcatcg cggtggtcgg
     2941 gtcgatcccc gaaagcgcga cggcgacgac gctgatcgac gcggccggcc ggatcgtcgc
     3001 gccgggcttc atcgacccgc attcgcatgc ggcgccgaat atccagacca aggagctcgc
     3061 cgccgcgctc ccgatgctgc accaggggat cacgacgctg gcgatcaatc ccgacggcgg
     3121 cgggccggcc gagctcggcc agcagatcgc cgacctcaac accaatatcc ccggggtcaa
     3181 cgtcattccg ctgatcggcc acaacgcggt gcggcgcgcg gtgatgggcg atgtcgcacg
     3241 gctcgcgacc gccgacgagc aggcgaaaat ggaagggctc gtcaccgcgg cgatggcggc
     3301 gggcgcctat ggcctgtccg acgggccctt ctacattccg ggcaaatatt caaacaccgc
     3361 cgagatcgtc gggctcgcga aggccgcggc gcactttccc ggcgcctttt acatcagcca
     3421 tgtccgcgac gagggcaatt acgacatcgg cgtcgtcgcc gcggtcgacg aggtgatcac
     3481 cgtcgcgcgc gaggcgaaaa ttccgggcgt cgtcacccat atcaaggtgc tcgggccgca
     3541 ggtgtggggc aagtcggccg acgtcatcgc ccgcatcgac caggcgcgcg ccgaggggct
     3601 cgaaatctgg gccgaccaat atccttactc tgcatcggga tcgagcctga tggcctcgct
     3661 cgttcccggc tgggcgcagg aagggggtgc ggaggcgctg gccaagcgcc tgcgggatcc
     3721 cgcgatgcgc gcgaaaatcc gggcggagat ggtgccgaac ctcgaacggc gcgccgggcc
     3781 gcacgcgctg atgatccgca gctttgcgcc cgatccctcg ctcgagggca aaaggctcga
     3841 cgaggtcggc cggatcaagg gcatggcccc gctcgacgcc gcgatcgaga tgctgatcgg
     3901 gggcggcgcg ccgacgatct cgttcaacat gagcgaggcc gatgtcgagg cctttatgcg
     3961 ccagccgtgg acgatgacct cgaccgacgg cgggctgccg catttcggca agacgtccga
     4021 gcatccgcgc gcctatggcg cctttccgcg caagctgcgc aattatgtgc tcgacaagcc
     4081 ggtgatcccg atggcgcagg cgatccatgc cgcgaccggc ctgcccgcgc agatcctcgg
     4141 cgtgcccgac cgcggcctgc tgcgcgaagg cgctttcgcc gacgtgatcg tctttgaccc
     4201 cgagaccata ggcgaccttg cgacctatga acggccgcac gcctattcgg tcggcgtgga
     4261 ttatgtcttc gtcaacggaa aggcggcgct ggcgaaagga aaacccaccg ccgagcgcca
     4321 cggccgcgtg ctgctgcggc cgcgctgacg ccgggagccc cgccttgctg cgacgcgaaa
     4381 catcttgccg cggggcgggg cgcggctaac catggcgggg atgaacctgc gccatatcga
     4441 gatattccac gcggtctata ccaacggatc ggtcagcgcc gccgcgcgcg cgctcaatat
     4501 ttcgcagccg tcggtgtcga agacgttgcg ccacgccgaa tcgatcctcg gctttccgct
     4561 gttcgaacgc tcggccgggc gcctcgtcgc gaccgacgac gcgcacgcgc tgttcgccga
     4621 ggtcagcgaa atcca
//
LOCUS       DATMSU010000112         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_40121, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000112 DATMSU010000000
VERSION     DATMSU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40121"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1039
                     /gene="fmt"
                     /locus_tag="VN034_02970"
     CDS             119..1039
                     /gene="fmt"
                     /locus_tag="VN034_02970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWV59589.1"
                     /translation="MRIAFMGTPPFAVPTLAALHAAGHDIVAVYSQPPRPAQRGKKLQ
                     KSPVQVWAEEHDLPVRTPKSLKSEEAQAEFAALDLDLAVVAAYGLILPQAVLDAPREG
                     CLNVHGSILPRWRGAAPVQRAILSGDAETGVTIMQMDAGLDTGAMRLIETTPTGRKSA
                     GVLTHELAEMGALMMRRVLSDLHAFPPVPQPEEGVTYAAKIDKSEARLDFLTSSVQVE
                     RQVRAFNPVPGAFFELEGERYKILAAEVVHPSDTVAGAAPGVTLDDALTIACNPGAIC
                     ATRVQRAGKPAMEASELLHGRPIPKGTRLT"
     gene            1185..1925
                     /gene="truA"
                     /locus_tag="VN034_02975"
     CDS             1185..1925
                     /gene="truA"
                     /locus_tag="VN034_02975"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWV59590.1"
                     /translation="MTRFALTIEYDGRPYMGWQRQAHGPSVQQTIEEAIHRFTAEDVQ
                     VFSAGRTDAGVHAIAMRAHVDIEKPLTPFKLTEALNAQLRPAPVAVVGCEIVPGDWHA
                     RFACIGRAYEYRIVNRRAPLTWDKGLSWQVARPLDTDAMHAAAQQLIGLHDFTTFRSA
                     HCQSQSPVKTLDKLTVNRHGDEVIIEAAARSFLHHQVRSMVGCLTLVGHGKWSARDLK
                     AALEAADRNALGLNAPPDGLYFVSATYP"
     gene            complement(2142..2669)
                     /locus_tag="VN034_02980"
     CDS             complement(2142..2669)
                     /locus_tag="VN034_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWV59591.1"
                     /translation="MSASTPVFEVGDYVVYPKHGVGRVIELQKSEIAGMQLELYVLRF
                     EKEKMTLRVPTNKAEGVGMRKLSSDKTLKEALQVLTTKPKVKRTMWSRRAQEYEAKIN
                     SGDLVSIAEVTRDLFRADDQPEQSYSERQIFEAASSRLARELAAMEESDEKTAQAKIL
                     QILNEHAPLYYAEKV"
     gene            2983..3795
                     /locus_tag="VN034_02985"
     CDS             2983..3795
                     /locus_tag="VN034_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59592.1"
                     /translation="MKGQIWWVTGASSGIGAALARALAARGAKLILSGRNVAALEAVA
                     ADCDTEALILPFEATDYAALPAIAEQAWAWRGRVDGLVNNAGISQRSLAIETDFSVYE
                     RIVAVDLLAPIALTQQLLPRMVGAGGGQIIAISSVAGIAGVPLRSAYSAAKHGIIGYH
                     DAVRAENEHLGLKVLVVAPGSVRTNVSRNALNADGSVRGESDAAIDNGLSPDDAAAQM
                     LAAVDAGTRELVVAEGAEAMIAALRRGDPDALFDRMSAMVQAGYAQQMKAQR"
     gene            complement(3870..5987)
                     /locus_tag="VN034_02990"
     CDS             complement(3870..5987)
                     /locus_tag="VN034_02990"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HWV59593.1"
                     /translation="MNPVLIAIACGLLAVLYGFITSRQVLSASAGNEKMQEIAGAIQE
                     GAKAYLGRQYRTIAFVGVAVAILVWWFLGAVSATGFLIGAVLSGVAGFVGMNISVKAN
                     VRTAAAAQTGLQAGLTMAFRAGAITGMLVAGLALLAISVFFWYLIGPGGFTVGGEDRT
                     VVDALVALAFGASLISIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDCAGMAADLFETYVVTVGATMVLIALLLKGTGDMLLPLMTLPLLMGGVCIVT
                     SIIGTYFVRLGSGTNVMGAMYKGFLVTAVLSIPAIWFSTSYALGGDMNTNIAGTDFNG
                     SDLFYCSLLGLVITGLIIWITEYYTGTNYRPVRSIAKASETGHGTNVIQGLAISLEST
                     ALPTLVICVGIIVAYQTAGIIGIAFAATAMLALAGMVVALDAYGPVTDNAGGIAEMAG
                     LDDSVREKTDLLDAVGNTTKAVTKGYAIGSAGLAALVLFGTYTADITEYSAVLGLDAP
                     LTFSLSSPYVIVGLLLGALLPYLFGAMGMTAVGRAAGDVVKDVRDQFANNKGIMDGTS
                     RPDYARTVDLVTKAAIKEMIVPSLLPVLAPIVVFFVIRAVAGPAAALEALGALLLGVI
                     VGGLFVAISMTSGGGAWDNAKKYIEDGNHGGKGSEAHKAAVTGDTVGDPYKDTAGPAV
                     NPMIKITNIVAILLLAALAHGAA"
BASE COUNT         1107 a         1976 c         2002 g         1008 t
ORIGIN      
        1 acttgatccg gggtcccgct cagcgacggt aaagctggac cccggatcaa gtccggggtg
       61 acgaaatggg tggcgtcgat cttgcacaac cccgcaacct ccgccaaagg cgcagcccat
      121 gcgtatcgcc ttcatgggaa cgccgccctt tgcggtgccg acgctcgccg cgctccacgc
      181 ggcggggcac gacatcgtcg ccgtctacag ccagcccccg cgccccgccc agcgcggcaa
      241 gaaattgcag aagagcccgg tgcaggtctg ggccgaagag catgaccttc ccgtccgtac
      301 gccgaagagc ctgaagagcg aagaagcgca ggccgaattc gccgcgctcg acctcgatct
      361 cgccgtggtg gccgcctatg gtctgatcct gccgcaggcg gtgctcgatg ccccgcgcga
      421 ggggtgcctc aacgtccatg gctcgatcct gccgcgctgg cgcggcgcgg cgccggtgca
      481 gcgcgcgatc ctgtcgggcg atgccgaaac gggggtgacg atcatgcaga tggacgcggg
      541 gctcgacacc ggcgcgatgc ggctgatcga gacgacgccg accggacgca agagcgcggg
      601 cgtgctcacc cacgaactcg ccgagatggg cgcgctgatg atgcggcgcg tgctgagcga
      661 cctccatgcc tttccgccgg tgccgcagcc cgaggaaggc gtcacttacg cggcgaaaat
      721 cgacaagagc gaggcgcggc tcgatttcct gaccagctcg gtgcaggtcg agcggcaggt
      781 gcgcgcgttc aatccggtgc ccggcgcctt tttcgagctg gagggcgaac gatacaagat
      841 actggcggcc gaggtcgtcc atccctcgga tacggtcgcg ggcgccgcgc ccggtgtcac
      901 gctcgacgac gcgctcacga tcgcctgcaa ccccggcgcg atctgcgcga cgcgggtgca
      961 gcgcgcggga aagcccgcga tggaagcgag cgaactgctg cacggacggc cgattccgaa
     1021 gggcacacgg ctgacatgaa cccccgcttc ccccttcgtc accctgaact cgtttcaggg
     1081 tccatggccc gccgccccct tcagtgctgc gcaggattag aggacaggcc atggatgctg
     1141 aaacaagttc agcatgacga aattggctat attagctccc acgcatgacc cgcttcgccc
     1201 ttaccatcga atatgacggc cgcccctata tgggctggca gcggcaggcg cacggcccga
     1261 gcgtccagca gacaatcgag gaagcgatcc accgcttcac cgccgaggat gtgcaggtgt
     1321 tcagcgccgg gcgcaccgac gccggcgtcc atgcgatcgc gatgcgcgcg catgtcgata
     1381 tcgaaaagcc gctgaccccg ttcaagctga ccgaggcgct caacgcgcag ctccgccccg
     1441 cccccgttgc cgtcgtgggc tgcgagatcg ttcccggcga ctggcacgcg cgcttcgcgt
     1501 gcatcggccg cgcctatgag tatcgcatcg tcaaccgccg cgcgccgttg acgtgggaca
     1561 agggcctgtc gtggcaggtc gcgcggccgc tcgacaccga tgcgatgcac gccgcggcgc
     1621 agcaactgat cggcctccac gatttcacga cctttcggtc ggcgcattgc cagtcgcaaa
     1681 gcccggtgaa gacgctcgac aagctgacgg tcaaccgcca cggcgacgag gtgatcatcg
     1741 aggccgcggc gcgcagcttt ctgcatcatc aggtgcgctc gatggtcggc tgcctgacat
     1801 tggtgggcca cggcaaatgg tcggcgcgcg atttgaaggc ggcgctggag gcggcggatc
     1861 gcaacgcgct ggggctgaat gccccgcccg acgggctgta tttcgtgagc gcgacctatc
     1921 cttgactccc tctccccttg ggggagaggg cagcgagact tgcgaacttg ttcgctagtc
     1981 gcagcgggtg agggcctgtt gcatcgaggg agcagccctc tcccagcttc ggctaggcgg
     2041 cgagccgcca agccttcgca tccctctccc ccaaggggag agggacatag aaaaagggcg
     2101 gccctaccag accgcccttt ccttcatcgc tgtggacgcg attacacttt ttcggcgtaa
     2161 tagagcggcg catgctcatt gaggatctga aggatcttcg cctgcgcggt cttttcgtcg
     2221 ctttcttcca tcgcggcgag ttcgcgcgcg aggcggcttg aggccgcttc gaagatctgg
     2281 cgctccgaat agctctgttc gggctgatcg tcggcgcgga acaggtcgcg ggtcacttcg
     2341 gcgatcgaca cgaggtcgcc cgaattgatc ttcgcttcat attcctgcgc gcggcgcgac
     2401 cacatggtgc gcttgacctt cggcttggta gtcaggacct gcaacgcttc cttcagcgtc
     2461 ttgtccgacg acagcttgcg catgccgacg ccttcggcct tgttcgtcgg aacgcgaagg
     2521 gtcatctttt ccttttcgaa acggagaaca taaagttcca gctgcatacc ggcgatttcg
     2581 gacttttgca gctcgatgac gcgccccacg ccatgtttcg gataaacgac ataatcaccg
     2641 acttcgaaaa cgggagtgct tgcggacatt caagttcctt tcagtggcct tgccggggac
     2701 ggggaagcgc gtgaacagcg gtatatccgg ccctctatcg gagggggatc gggctggtag
     2761 ggcggcgcta gccagtcaaa gtgacaagga atgggctcca tcggaaacgc caagcgtgcc
     2821 ccagcccggc ggcgggaaga ggcacaatgt cggcgtgttg atatttatta taacagattc
     2881 gcaacaaaat tgccaccccc gcgcggaaat gcgcgggtcg acaccttgac ggctcctcac
     2941 acccgtttca ccttgaaacg aaatcataga tagggagccg gtatgaaagg tcaaatttgg
     3001 tgggtcacgg gggcctcctc gggcatcggc gcggcgctgg cgcgggcgct ggcagcgcgc
     3061 ggggcgaagc tgatcctgtc gggccgcaat gtcgcggcat tggaagccgt tgccgccgat
     3121 tgcgataccg aggcgctaat cctgccgttc gaggcgaccg attatgccgc cctccccgcg
     3181 attgccgaac aggcctgggc gtggcgcggc cgcgtcgacg gcctcgtcaa caacgccggc
     3241 atctcgcagc gcagtctggc gatcgaaacc gacttttcgg tttacgagcg gatcgtcgcg
     3301 gtcgacttgc tcgcccccat cgcgctcacc cagcaattgc tgccgcgcat ggtcggcgcg
     3361 ggcggcggcc agatcatcgc catatcgagc gtcgcgggca tcgcgggcgt gccgctgcga
     3421 agcgcctatt cggcggcgaa gcacggcatc atcggctatc acgacgcggt gcgcgccgag
     3481 aatgagcatc tggggttgaa agtgctcgtc gtcgcgccgg gatcggtgcg caccaacgtc
     3541 agccgcaacg cgctcaacgc cgacggcagc gtccgcggcg aaagcgacgc cgcgatcgac
     3601 aacggcctgt cgcccgacga cgccgcggca cagatgctcg ccgcggtcga tgccggcacg
     3661 cgcgaactgg ttgtcgccga gggcgccgaa gcgatgatcg cggccttgcg ccgcggcgat
     3721 cccgatgcgc tgttcgaccg gatgagcgcg atggtgcagg caggctatgc acagcagatg
     3781 aaggcgcagc gctaaaatca cgcgactgac aaggaaagcc ccgccggatc gctccggcgg
     3841 ggcttttcat tgcccgcagg cggcgcagcc tatgccgcgc cgtgcgccag cgccgcgagg
     3901 agcaggatcg ccacgatgtt ggtgatcttg atcatcgggt tgaccgcggg acccgcggta
     3961 tccttgtagg gatcgccgac ggtgtctccg gtcaccgcgg ccttatgggc ctcgctgccc
     4021 ttgccgccgt ggttgccgtc ctcgatatat ttcttcgcat tgtcccatgc gccgccgccc
     4081 gaggtcatcg agatggcgac gaacagcccg ccgacgatca cgccgagcag cagcgcgccg
     4141 agcgcctcga gcgccgccgc cggaccggcg accgcacgga tcacgaagaa caccacgatc
     4201 ggtgcgagca ctggcagcag gctgggcacg atcatttcct tgatcgccgc cttggtgacg
     4261 aggtcgacgg tgcgggcgta gtcggggcgg ctggtgccgt ccatgatgcc cttgttgttc
     4321 gcgaactggt cgcgcacgtc cttaaccacg tcacccgcgg cacggccgac cgcggtcatg
     4381 cccatcgcac cgaacaggta cggcaggagc gcaccgagca gcagcccgac gatgacgtac
     4441 ggcgacgaca gcgagaaggt cagcggcgcg tcgagcccga gcaccgccga atattcggtg
     4501 atgtcggcgg tgtaggtacc gaacagcacc agcgccgcaa ggcccgccga accgatggca
     4561 tagcccttgg tcaccgcctt ggtcgtgttg cccaccgcgt cgagcaggtc ggtcttttcg
     4621 cgcacgctgt cgtcgaggcc cgccatttcg gcgatgccgc cggcgttgtc ggtgacgggg
     4681 ccataggcgt cgagcgcgac caccatgccc gccagcgcaa gcatcgcggt ggcggcgaag
     4741 gcgatgccga tgatgcccgc ggtctgatag gcgacgatga tgccgacgca gatgacgagc
     4801 gtcggcagtg cggtcgattc aaggctgatt gcgaggccct ggatcacgtt ggtgccgtgg
     4861 cccgtttctg aagccttggc gatcgaacga accgggcgat agttggtgcc ggtgtaatat
     4921 tcggtgatcc agatgatcag cccggtgatg acgagaccga gcagcgagca atagaaaagg
     4981 tcgctgccgt tgaagtcggt gcccgcgata ttggtgttca tgtcaccgcc cagcgcatag
     5041 ctggtcgaga accagatcgc cgggatcgag aggaccgccg tgacaaggaa gcccttgtac
     5101 atagcgccca tgacgttcgt cccgctgccg agacgaacga aataggtgcc gatgatcgag
     5161 gtgacgatgc aaacaccgcc cattagcagc ggcagcgtca tcagcggcaa cagcatgtcg
     5221 ccggtgccct ttagcagcaa cgcgatcaac accatcgtgg cgccgacggt gacgacatag
     5281 gtttcgaaaa ggtcggccgc catgccggcg cagtcgccga cattgtcgcc gacgttatcg
     5341 gcgatcaccg cggggttgcg cggatcatcc tcggggatgc ccgcctcgac cttgccgacg
     5401 aggtcggcgc cgacgtcggc cgccttggtg aagatgccgc cgccgagacg cgcgaagatc
     5461 gagatcagcg aagcaccgaa cgccagcgcg acgagcgcgt cgacaacggt ccggtcctca
     5521 ccgccgacgg tgaagcctcc ggggccgatc agataccaga agaagaccga gatcgcgagg
     5581 agcgcgagcc ccgcaacgag catgccggtg atcgcgccgg cgcggaacgc catggtgagg
     5641 cccgcctgca gcccggtctg cgccgccgcc gcggtgcgga cgttggcttt caccgagatg
     5701 ttcatgccga cgaagccggc gacgcccgac aggacggcgc cgataagaaa gcccgtggcc
     5761 gaaaccgcgc cgaggaacca ccagacgagg atggcgacgg cgacgccgac gaaggcgatg
     5821 gtgcgatatt ggcggcccag ataggccttg gcgccttcct ggatggcgcc ggcgatttcc
     5881 tgcatcttct cattacccgc cgatgcgctg agcacctgcc gcgaggtaat gaacccatag
     5941 agcacggcca acaggccgca cgctatcgcg atcaaaaccg gattcatgcg tgttgcttcc
     6001 ttcccatgag aagggcgacg cagaagatag cagtcgagca cgtccgactt attgatgccg
     6061 gttcgctctc tcccctgatg cgaccgcccc aaa
//
LOCUS       DATMSU010000113         6345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_40129, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000113 DATMSU010000000
VERSION     DATMSU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40129"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VN034_02995"
     CDS             complement(<1..1364)
                     /locus_tag="VN034_02995"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HWV59594.1"
                     /translation="MTISLFELFKIGVGPSSSHTVGPMVAARRFTVWLDEAELLGKTA
                     HVEADLYGSLALTGKGHAADTAIVAGLAGEIPASTSLAAIKAHWDRAESAGFIYLLGR
                     QRVRFQPKGDLHFQMRQRQPFHSNAVSFIARDSDGEILAKRMYFSIGGGFVVDEDEAG
                     RNSRGAEDEVELPFAFTSGADLLEMGAASGKSFAEMMLENELAHRSLEEVEAGLDAIA
                     GAMDASIDAGCSSTGVLPGGLKVKRRAPQIADDIRNRHEANLTDPLAMMDWINLWAMA
                     VNEENAAGGKVVTAPTNGAAGIIPAVIRFYRRGYRGDDAGVRTFLLAAGAVGALYKRN
                     ASISGAEVGCQGEVGVACSMAAAGLTAALGGTNAQVENAAEIGMEHNLGLTCDPIGGL
                     VQIPCIERNAMGAIKAIDASRIAMIGDGTHVVSLDTVIATMLQTGRDMRDKYKETSKG
                     GLAV"
     gene            complement(1570..2184)
                     /locus_tag="VN034_03000"
     CDS             complement(1570..2184)
                     /locus_tag="VN034_03000"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HWV59595.1"
                     /translation="MTIAHPPLPYAQDALEPHISADTLATHHGKHHKAYVDKTNAAIE
                     GTDLASKPLEEIVHHAEGSGNKGLFNNAAQAWNHAFYWESLSPSKTEPAGDLAAAIER
                     DFGSLGDLKKKLKETAVNHFASGWAWLVSRDGTLSVTDTHDAGTELVAGIKPLLVIDV
                     WEHAYYIDRKNLRPAYVDAVVDELLNWDFAAENLARESTWTYPA"
     gene            complement(2398..4062)
                     /locus_tag="VN034_03005"
     CDS             complement(2398..4062)
                     /locus_tag="VN034_03005"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HWV59596.1"
                     /translation="MKRFAALIDRLIYTRSRNTKLALIVDYLRHTPDPDRGWAIAALT
                     ESLDFPAVKAGTVRALLASRVDEELFRLSRHFVGDTAETAALLWPEPEKPLPFRGGVG
                     GGVSKAGATSLDPTPTPPLKGRGLSVSEAVDALSATSRSDAPAVVASLLDRLDADGRY
                     ALLKLALGGMRVGVSARLAKQAFAQAFDVPVDDVEELWHAIPPPYAPLFAWGEGRAER
                     PDLADVAFFRPFMLAHPLEAESIDLADYAAEWKWDGIRVQIVRGGSGGGGETRIYSRG
                     GEEISGAFPELVAAFDQDAVIDGELLVRGEVQGGEAASFNALQQRLGRKVVSKKMLAD
                     YPAFVRVYDLLGVDGNDLRGLPWTERRRQLEAFVPRLADSHFDLSQVIDARDFDDLAA
                     RRAGARDAAIEGVMLKRRDAPYVAGRRAGLWYKWKRDPLTADCVMMYAQRGNGRRASF
                     YSDYTFGCWSDAGELLPVGKAYSGFTDEELKWLDKFVRDNTLNRFGPVREVEKSLVLE
                     VAFDSIHGSKRHKSGLAMRFPRISRIRRDKPAEEADRIATLQAMVT"
     gene            complement(4059..5075)
                     /locus_tag="VN034_03010"
     CDS             complement(4059..5075)
                     /locus_tag="VN034_03010"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HWV59597.1"
                     /translation="MAKAKHWIEPHPSGIYVKPADLWIDPSRPVERAAVTHGHADHAR
                     SGHDAVFATPETLAIMALRYGVDAEASHNQAFVYGDGFERGGVRFSFHPAGHVLGSAQ
                     ILMEYAGERIVVTGDYKRRADPTCARFEVVPCDIFITEATFGLPVFRHPPTGDEIAKL
                     IGAVRAEPDRSVLVGAYALGKAQRVIAELRGAGWDAPIYIHGALEKMCQLYAIHGVDL
                     GELRLVSETDKAEMAGQIILAPPSALNDRWSRRLPDPVTAMASGWMRVRQRARQRMVE
                     LPLVISDHADWDELTATIEEVNPKESWITHGSEDGLLRWCELTQRKARALHLVGRDLE
                     EEEA"
     gene            complement(5075..>6345)
                     /locus_tag="VN034_03015"
     CDS             complement(5075..>6345)
                     /locus_tag="VN034_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052579.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HWV59598.1"
                     /translation="GAVALPRELLEIAERVICHRGAEVPARLYRVIWRAARDRLLLAR
                     TTDEDVDWLRKADKAIRRDVHKMHAFVRFRRLGEEAGRERFAAWFEPSHRIVRLTAPF
                     FQRRFTGMDWAIVTPDARAIWRGETLIYGPGGTRDEVPDSDVIEDQWRTYYGAIFNPA
                     RVKIDAMRAEMPKKYWKNLPEAQDIAPLLAGAEARVERMREAAVSMANPLTEKWRTRV
                     VQELALDDDVTTLGDLARAVDRCTRCPLYCNATQGVAGEGPKAARIMLVGEQPGDQED
                     LQGRPFVGPAGQVLNEALDEAGLDRRRLFLTNAVKHFKFEPRGKRRLHQNPTTAEIDI
                     CRWWLDKERALVKPDLIVTLGVSALRGVTGKSASIKSMRGATHELEGGVKLIATIHPS
                     FLLRMPDRARAAEERKAFVADLIRARKLAA"
BASE COUNT         1027 a         2198 c         2037 g         1083 t
ORIGIN      
        1 ctgaccgcca gcccgccctt cgacgtttcc ttatatttgt cgcgcatgtc gcggccggtt
       61 tgcagcatcg tcgcgatcac cgtgtcgagg ctgacgacat gcgtgccgtc gccgatcatc
      121 gcgatgcggc tggcgtcgat cgccttgatc gcgcccatcg cattgcgctc gatgcacggg
      181 atctgcacca gtccgccaat gggatcgcag gtgaggccga ggttatgctc catcccgatc
      241 tcggcggcgt tttcaacctg cgcgttggtg ccgcccaatg ccgccgtcag ccccgccgcc
      301 gccatcgagc aggcgacccc gacctcgccc tggcaaccga cctcggcgcc cgagatgctg
      361 gcgttgcgct tgtagagcgc gccgaccgcg cccgccgcga gcaggaaggt ccgcaccccc
      421 gcgtcatcgc cgcgatagcc gcgccggtag aagcggatca ccgcgggaat gatccccgcc
      481 gcgccattgg tcggcgcggt gacgaccttg ccgcccgcgg cgttctcctc gttcaccgcc
      541 atcgcccaca ggttgatcca gtccatcatc gcgagcggat cggtcagatt cgcctcgtgg
      601 cggttgcgga tgtcgtcggc gatctgcggc gcgcggcgct tgaccttgag cccgcccgga
      661 aggacgcccg tgctcgagca tcccgcgtcg atcgatgcat ccatcgcccc ggcgatcgcg
      721 tcgagccccg cctcgacctc ttcgaggctg cggtgcgcga gctcgttttc gagcatcatc
      781 tcggcgaaac tcttgcccga ggcagcgccc atttcgagca ggtcggcgcc cgaggtgaag
      841 gcgaagggca gttcgacctc atcctcggcg ccgcggctgt tgcgccccgc ctcatcctca
      901 tcgacgacga agccgccgcc gatcgagaaa tacatgcgtt tcgcgaggat ctcgccgtcg
      961 ctgtcgcgcg cgatgaagct cacggcattg ctgtggaaag gctggcgctg gcgcatctgg
     1021 aaatgcagat cgcctttcgg ttgaaagcga acacgctggc ggccgagcag gtagataaag
     1081 cctgcgctct cggcacggtc ccaatgcgcc ttgatcgcgg cgaggctggt cgacgccgga
     1141 atctcgccgg cgagccccgc gacgatcgcg gtgtcggcgg catgaccctt gccggtcaac
     1201 gcgagcgagc catagagatc ggcttcgaca tgcgctgtct tgcccagaag ctcggcctcg
     1261 tcgagccaga cggtgaaacg ccgcgcggcg accatcgggc ccacggtatg cgagctggaa
     1321 ggaccgacac caattttgaa gagttcgaac aggctgatcg tcatggcggc cctataggag
     1381 aaggcggacg atcccgatag gcccacgcgg gacaattttc gttacggcct gcaagaaatc
     1441 ctcccgtcgc gcagcgatgg ggaggtggca gcgcggaacg ctgacggagg ggctgcaacg
     1501 ccgcggcccc tccaccatgc ttcgcatagt tcccctcccc accgcttcgc ggcagggagg
     1561 atcggatcgt tacgccggat aggtccaggt cgactcgcgc gcgagattct cggcggcgaa
     1621 atcccagttg agcagctcgt cgaccaccgc gtcgacatat gccgggcgca gattcttgcg
     1681 gtcgatataa taggcatgct cccacacgtc gatcacgagc agcggcttga tgccggcaac
     1741 cagctcggtg ccggcgtcgt gcgtgtcggt gaccgacagc gtgccgtcgc gcgagacgag
     1801 ccacgcccag cccgacgcga aatggttgac cgcggtctcc ttgagcttct ttttcaggtc
     1861 gcccagcgag ccgaaatcgc gctcgatcgc ggcggcgagg tcgccagccg gttcagtctt
     1921 cgacgggctc aggctttccc aataaaaggc gtggttccac gcctgcgccg cgttgttgaa
     1981 caggcccttg ttgcccgatc cttcggcgtg gtggacgatt tcttcgagcg gcttcgaggc
     2041 gagatcggtg ccttcgatcg cggcgttggt cttgtcgaca taggccttgt ggtgcttgcc
     2101 atggtgcgtc gccagcgtgt cggccgagat atgcggctcg agcgcgtcct gggcataagg
     2161 caggggagga tgggcgatcg tcatggcagt ctcctttttt atgaattcgg gcccttaact
     2221 cggtatcacg gccgcgggtt gcaaccccgg gacccggaaa aatctatatc cccctcccta
     2281 ttagggaggg gttaggggtg ggtcgccgca ggcgttcttc cttgccgcat accccccccc
     2341 gctgcggcta ggcagcaagc tgccaactct tcgcacccct ccctaaaggg gggggcttta
     2401 ggtcaccatc gcctgcaacg tcgcgatccg gtcggcttcc tcggcgggtt tgtcgcggcg
     2461 gatgcgcgat atgcgcggga agcgcatcgc gagccccgac ttgtggcgct tgctgccatg
     2521 gatcgaatcg aaggcgactt cgagcacgag cgacttttcg acctcgcgca ccgggccgaa
     2581 gcggttcagc gtgttgtcgc gaacgaattt gtcgagccat ttcagctcct cgtcggtgaa
     2641 gcccgaatag gccttgccca caggcagcaa ctcgcccgcg tcggaccagc agccgaaagt
     2701 atagtccgaa tagaagctcg cgcggcgccc gttgccgcgc tgcgcgtaca tcatcacgca
     2761 gtcggcggtg agcgggtcgc gcttccattt ataccagagc ccggcgcggc gccccgccac
     2821 gtaaggggca tcgcggcgct tgagcatcac gccttcgatc gccgcatcgc gcgcacccgc
     2881 gcggcgtgcg gcgagatcgt cgaagtccct cgcatcgata acctgcgaaa ggtcgaaatg
     2941 actgtcggca agccgcggca cgaaggcttc caattgccgc cgccgctcgg tccacggcag
     3001 gccgcgcagg tcgttgccgt cgacgccaag cagatcatag acgcgcacga aggcggggta
     3061 gtcggcgagc atcttcttcg agacgacctt gcgccccagc ctttgctgca gcgcgttgaa
     3121 gctcgccgcc tcacccccct gcacctcgcc gcgcacgagc agttcgccgt cgatcaccgc
     3181 atcctgatcg aacgccgcga ccagttcggg gaaggcccca ctgatctcct cgccgccgcg
     3241 gctgtagatg cgcgtctcgc cgccgccccc gctgccaccg cgcacgatct ggacgcggat
     3301 gccgtcccat ttccactcgg cggcatagtc ggcgaggtcg atgctctccg cttcgagcgg
     3361 atgcgcgagc atgaaggggc gaaagaaggc gacgtcggcg aggtcgggcc gctccgccct
     3421 tccctcgccc cacgcgaaca gcggcgcata gggcggcgga atcgcgtgcc acagctcctc
     3481 gacatcgtcg acgggcacgt cgaacgcctg cgcgaacgcc tgtttggcga gccgcgccga
     3541 caccccgacg cgcatgccgc ccaaagctag cttgagcaaa gcatagcgcc cgtcggcgtc
     3601 gagccggtct agcagcgagg caacaacggc gggcgcgtcg ctgcggctcg tggcagagag
     3661 ggcgtcgacg gcttcggaca cgcttaagcc cctccccttc aggggagggg ttggggtggg
     3721 gtccagcgag gtagcgccag ccttcgaaac cccaccccca acccctcccc tgaaggggag
     3781 gggcttttca ggctccggcc acagcagcgc cgccgtctcg gccgtatcgc cgacgaagtg
     3841 ccgcgacagg cggaacaatt cctcgtcgac ccggcttgcg agcagcgcgc gcaccgtccc
     3901 cgccttcacc gccggaaaat cgagactttc ggtcagcgcc gcgatcgccc aaccgcggtc
     3961 gggatcaggg gtgtggcgga ggtaatcgac gatcagcgcg agcttggtgt tgcgcgagcg
     4021 cgtgtagatc agccggtcga tcagggctgc gaagcgcttc acgcctcttc ctcctccaga
     4081 tcgcggccaa cgaggtgcag cgcgcgcgcc ttgcgctgcg tgagctcgca ccagcggagc
     4141 agcccgtcct cgctgccgtg ggtgatccag ctttccttcg ggttcacctc ctcgatcgtc
     4201 gcggtgagct cgtcccagtc ggcatggtcc gaaatcacaa gcggcagttc gaccatccgc
     4261 tgccgcgcgc gctggcgcac gcgcatccag cccgacgcca tcgcggtcac cggatcgggc
     4321 agccggcgcg accagcgatc gttcagcgcc gacggtggcg cgaggatgat ctgtcccgcc
     4381 atctccgcct tgtcggtctc cgaaacgagc ctgagctcgc caaggtcgac gccgtggatc
     4441 gcatagagct ggcacatttt ctcgagcgcg ccgtggatat agatcggcgc atcccatccg
     4501 gcgccgcgca attcggcaat cacgcgctgc gccttgccga gcgcataggc gccgacgagc
     4561 accgagcgat ccggctcggc gcgcaccgcg ccgatcagct tcgcgatctc gtcgccggtc
     4621 gggggatggc ggaacacggg caggccgaag gtcgcctcgg tgatgaagat atcgcacggc
     4681 acgacctcga agcgcgcgca ggtcgggtcg gcgcgccgtt tgtagtcgcc ggtgacgacg
     4741 atccgttcgc ccgcatattc catcaagatc tgcgcgctgc cgagcacatg gcccgcgggg
     4801 tggaagctga aacgcacccc cccacgctca aacccgtcgc cgtagacgaa ggcctgatta
     4861 tgactcgcct cggcatcgac gccatagcgc agcgccatga tcgcgagcgt ctcgggggtc
     4921 gcgaataccg cgtcatggcc gctgcgcgca tggtcggcat gaccgtgcgt caccgcggcg
     4981 cgctcgaccg ggcgcgacgg gtcaatccac aaatcggcgg gcttcacata gatgccgctc
     5041 ggatgcggct cgatccaatg cttcgccttt gccatcaggc tgcgagcttt cgcgcgcgga
     5101 tcaggtcggc cacgaatgcc tttcgctcct ccgccgcacg cgcgcggtcg ggcatacgga
     5161 gcagaaaaga cggatggatc gtcgcgatca gcttcactcc cccttcgagt tcgtgcgtcg
     5221 caccgcgcat cgacttgatg ctcgcactct tgcccgtcac cccgcgcagc gcgctcacgc
     5281 caagcgtcac gatcaggtcg ggcttcacca gcgcgcgttc cttgtcgagc caccagcggc
     5341 agatgtcgat ctcggcggtc gtcggattct ggtgcagccg ccttttgccg cgcggctcga
     5401 acttgaaatg cttgaccgcg ttggtgagga acagccgccg ccggtcgagc ccggcttcgt
     5461 cgagcgcctc gttcagcacc tgccccgccg ggccgacgaa ggggcgccct tgcagatcct
     5521 cctgatcgcc cggctgctca ccgacgagca tgatgcgcgc cgctttcggc ccctcgcccg
     5581 cgacgccctg ggtggcgttg caatagagcg gacagcgcgt gcagcggtcg accgcgcgcg
     5641 caaggtcgcc cagcgtcgtc acatcgtcat cgagggccaa ttcttgcacc acgcgcgtcc
     5701 gccatttttc ggtcagaggg ttcgccatgg aaacggccgc ttcgcgcatg cgttccaccc
     5761 gcgcctcggc acccgcgagc aacggcgcga tatcctgcgc ctcgggaagg ttcttccaat
     5821 atttcttcgg catctcggcg cgcatcgcgt cgatcttcac gcgcgcgggg ttgaagatcg
     5881 cgccataata ggtgcgccac tgatcctcga tcacgtcgct gtcgggcacc tcgtccctgg
     5941 tcccgccggg gccgtaaatc agcgtctcgc cccgccagat cgcgcgcgca tcgggggtga
     6001 cgatcgccca gtccatgccg gtgaagcgcc gctgaaagaa gggcgcggtc agccggacaa
     6061 tgcggtgcga cggttcgaac cacgccgcga aacgctcgcg ccccgcctcc tcgccaagcc
     6121 gccggaagcg cacgaaagcg tgcatcttgt ggacgtcgcg gcggatcgcc ttgtcggcct
     6181 tgcgaagcca gtcgacatcc tcgtcggtcg tgcgcgccag aagaagacga tcgcgcgccg
     6241 cgcgccagat cacgcgatag agccgcgccg gaacctcggc accgcgatga cagatcacgc
     6301 gctcggcgat ttcgagcagt tcgcgcggca gcgcgaccgc gccgg
//
LOCUS       DATMSU010000114        18963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_68520, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000114 DATMSU010000000
VERSION     DATMSU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 18963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18963
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68520"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VN034_03020"
     CDS             <1..787
                     /locus_tag="VN034_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59599.1"
                     /translation="LPAFAGATEGNMTTAKLFIHGVPDSPAIWRPLLAALELGDTPVA
                     VPALPGFTAPLPPGFPATKEAYADWAVGEAEALVAAHGTIDIVGHDWGALIAQRVAML
                     RPDLIRSWAISNAVIDPDYRGHRIARIWNTPILGEIFMALSKADKLAEGLAAQGMPAD
                     IAREEAGQWKHKDKRRAILKLYRSAKGLSFAHDWALDIPKLPANGALIWGEGDPYVEL
                     SVARRYATNTGTLLTVIEGAGHWAIAERPVEVAAALHAFWNGL"
     gene            1018..1503
                     /locus_tag="VN034_03025"
     CDS             1018..1503
                     /locus_tag="VN034_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HWV59600.1"
                     /translation="MGAADQRFFDALRAAHFPPERNHLAAHITLFHQLPPSCLDELDR
                     LFRRIAADTPPPAAMLREVYSLGQGVAFRIESPELLDVRARIADHFAGMLTAQDRGVP
                     RLHITVQNKVAAAEARALLAALACDFRPRPLTITGLAAHRYRGGPWEPAFARSFRAMR
                     R"
     gene            1565..1641
                     /locus_tag="VN034_03030"
     tRNA            1565..1641
                     /locus_tag="VN034_03030"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1599..1601,aa:Met,seq:cat)
     gene            1832..3190
                     /locus_tag="VN034_03035"
     CDS             1832..3190
                     /locus_tag="VN034_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HWV59601.1"
                     /translation="MALKDLEIKYAARRSRDYKLFDGEGLYVLVTPRGSKLWRLKYFF
                     DGKEKTLSLGKYPAISAAAARQMRREAKKLVADGIDPGEHRRSNKGRLPPQTLFEPIA
                     RAWHANRCEALDAAHASRVMSRLERDVFPVLGQRDIKSITAPEILEVVRAVEARGALD
                     VSRRVKQSIGQVFRFAIASGWATNDPTTSINDALRPRPRVKHMPKVPLAELPELVAAI
                     RSYDGEADARRRATTRDAMMFTLLTWARTSETRFASWDEIDDLDGEMPLWRLPAERMK
                     MHREHVVPLSSQVVELLLRRRARGGHYIFEGDKPGHPISENTMIFACYRMGYRGRQTV
                     HGFRGLASTWANEAECFKPDWIEMALAHADEDEVRGAYNSALYMSPRRRMLQRWADEI
                     YQESPLVTPRAQMRSSLRQVSKATPGTRGEARSERLSSERLPFWQRPDGRRGLPKPRF
                     GS"
     gene            3453..4592
                     /locus_tag="VN034_03040"
     CDS             3453..4592
                     /locus_tag="VN034_03040"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWV59602.1"
                     /translation="MAVARAKVTARTPLIKARAVLDELLNRIDRFNADPEAVSRVDEI
                     WLFGSMMREEATVGDIDLAICRSRDARFADDLDGQVEQAKRLIQGYPDAPQRWAWPWD
                     RIDWLYRRSIFGPRRHPLLAGAQEGTTDLQLLAVPCRLIYDRARGGRVSDPVLPRHPE
                     SPGRANTLFAPDELPDLTPGELRPMDARWIAGFKPWGAVSPYDIFRGWTDEGERLFGS
                     YPEHLRIVADGHDLRGFPWIPKPVSKGGLDGRNAIAIVNAADWCGTSITLRRAIEGRP
                     GSRGLHASFTDLALYRRRKHVEISTLFDMVSAVSIILAVDAERILRRATESGEPREVT
                     IRISSDDLPEDMRRHFAQEIADMLARRAVAIEPAGETASVHIQLA"
     gene            4672..5796
                     /locus_tag="VN034_03045"
     CDS             4672..5796
                     /locus_tag="VN034_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59603.1"
                     /translation="MTARGKGGRAGSTINTRSPRMKYFRLLGWSPYNSPSEYIVHLCK
                     ELGRLGIAIEDIETHSSPPNGEEVLSELVAWLAEHPGANYSALVDWKVLDIANLADRM
                     RSNPDAKPRIEITAEITKMRNLSVRWYPAGWHDAMPDWSGRDPFNMIGHKVWDTLPDF
                     RWFRGDQPATFDGRATNELMFIRGVGNLHPEPRNEYGFYAKFNHDLVDGMLIAAVRAA
                     YERLIGQLSRSFDVEVLNAFDFEVRDEESDDDNSGGRSGRRVVGWALLDARELRARRE
                     REAEEAREAQGRADVAQIAARHNCTFAEFLQALDDAVHSKSNNCAASEQVYRVAAKSL
                     RKAGADINAGDVRRLVRIIGDFMSEELPASLRCDESAAAG"
     gene            complement(5878..6642)
                     /locus_tag="VN034_03050"
     CDS             complement(5878..6642)
                     /locus_tag="VN034_03050"
                     /inference="COORDINATES: protein motif:HMM:NF021581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6499 domain-containing protein"
                     /protein_id="HWV59604.1"
                     /translation="MSKGAPAADWRSAEAYAELLLCERRFFAWEWLRRNPAYRRDWNR
                     RDRLPGDASARMGLVAWIDPAIPAPRARPIWSVTTDPGVLDGRPAAGGNSGTTGDMLD
                     ILTVAPLVSVEVTARAEHWLLSDGHWAIRLDLHDGTLLGGPLLIEHRLTGLQSLTPKL
                     AALRQLAMIAEKGQLPAALMPRERRAAQWILELRVADALLAGASQQDMARHLFPLAVA
                     SPRWRVDATSYRLRVQRLVRKARRHLEDPFAGPWFE"
     gene            complement(6851..7825)
                     /locus_tag="VN034_03055"
     CDS             complement(6851..7825)
                     /locus_tag="VN034_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HWV59605.1"
                     /translation="MAAVEPEHAEAIAAALLGEYRSLSRMWSQSPEAHARVIGRRPAV
                     IALLASGRDALHETMRADFATRRIDPLDAALRRYLTVSMGSLPEERLRVLFLDPARGL
                     LADEQLQHGTLAHMSIYPRTILGRALELNAAAIILIHNHPSGDPKPSDADIQATDLLE
                     RVGRSLDIELLDHIVVTATRTHHLRSGKTKTVAADRPKSLALRSASPQPPGDTNDRIL
                     GNAKATLRRKILRRQLLGAADLFGEPAWDMLLDLFVHECEGKQLSMSSLCVAAAIPES
                     SAMRLAQRLCDAGLLCRVPDPGDGRRSFMKIAPDTAHRLRAYFAEGGE"
     gene            complement(7789..8181)
                     /locus_tag="VN034_03060"
     CDS             complement(7789..8181)
                     /locus_tag="VN034_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59606.1"
                     /translation="MAMILIAMLIADQALHLFVPARLDALDSGHLAIDLFAAIATMAL
                     ALTAHRFWPLAAAALQLLPLLAHFSRAADLSVHPAAYLTMQVFASWLLPPLLVLGTWR
                     HRKRLRTCGSDPSWHASWRRSNPSMPKP"
     gene            complement(8305..8631)
                     /locus_tag="VN034_03065"
     CDS             complement(8305..8631)
                     /locus_tag="VN034_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59607.1"
                     /translation="MSDTIAFVARKLQQQVPFAETRIDDALIAVSSLMTTVVTARRDM
                     GGAEPAREQGTILRLADAQRALIDASGGMLRVHGELSGIARERSGLDLHECPAVAQSA
                     DAQRTA"
     gene            complement(8687..9205)
                     /locus_tag="VN034_03070"
     CDS             complement(8687..9205)
                     /locus_tag="VN034_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV59608.1"
                     /translation="MEGSQSSTAADRAQWDRLTEKQRDCLDLLLERKTSKQIARQLGI
                     AKPTVDQRITAARTILGAIDRDDTARRYARLKQIYDRITYDPVHIPPPPALVPSDFPD
                     GDPSEPARTDVLSRARDGFLGEGLPFGKLGSHDHGSTTRLAIVAGMLATVIFALLGGL
                     GIAQALTQLISN"
     gene            9813..10409
                     /locus_tag="VN034_03075"
     CDS             9813..10409
                     /locus_tag="VN034_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-homoserine-lactone synthase"
                     /protein_id="HWV59609.1"
                     /translation="MLVVDQSTRAREHAVLRAMFAARKRVFVDLLKWDLPVLAGRFEV
                     DHRDGPDATYLIVAGPEGEHRASARLLPVCPACFTRAEGCLFQRGDRASGIAEVTHFC
                     VSPDIDGDARRHARDQLLHGLVDHALARRIHRLVGIAEEAWARRAEGFGWACRRVGPV
                     RAPFGRNMVTVVVDIDRTTPERLVAAGIVRSSAPRQAL"
     gene            10409..11032
                     /locus_tag="VN034_03080"
     CDS             10409..11032
                     /locus_tag="VN034_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWV59610.1"
                     /translation="MSPGATMERVYRELKARAMRGDFAPGERLDPAVLARDLGASVTP
                     LRDALHRLSGERFIDSWHQEGFRQPILAEADLHDLYEWAAALLTLALAGRKPDPSLPD
                     AAPNGTREPDYPAEVAGLFRAIASQSGNRELRFAVVNLIERSHVFRAAELHVDPGCSA
                     AIAAMEEDFRFRRWSALRSKVTRFHHRRAALAGRVVAAMRPREQPLG"
     gene            11163..11303
                     /locus_tag="VN034_03085"
     CDS             11163..11303
                     /locus_tag="VN034_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59611.1"
                     /translation="MDRHDIAEDGIEELGVASVETLGSAPPFPEEETLSPVPIGGGIT
                     AE"
     gene            11403..12005
                     /locus_tag="VN034_03090"
     CDS             11403..12005
                     /locus_tag="VN034_03090"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HWV59612.1"
                     /translation="MLDLLADRYLLLGPEVADVLTALGRCRSVFSVAVLEKLLARGLL
                     LPGPGATVGPVGQPCPLGSALEADLSGGGVSAFEALRRSIGALITMRALGLAPLIARS
                     RTVRARAARRTELVEDPDRAAFFARGFAEARISVPMPRLCVPDSLALAGSLWARGLAA
                     EVYFGVQLDPLLAHAWVQQGPLVLSDPLNIAADYRPVFRL"
     gene            12098..13678
                     /locus_tag="VN034_03095"
     CDS             12098..13678
                     /locus_tag="VN034_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWV59613.1"
                     /translation="MGEWTTPWWAGEGVLLVGWAFRRDGFGACTRLSSEEVERLVASE
                     GRWALAALWGQFILVWADPDGHIRLLRSPVDGPPIYHNVGERGACAFTDLALARSLGF
                     ALSGPDPMAVDAALRFPFLRAARTELAGVREILPGEIAELGDGHRQPGSWSPWDYAVR
                     PPRRIAREDLRTMVQAVTGAWSGRFGRVQLELSGGLDSSIVAMCLAGRRAAWRAITLA
                     TREPDGDERPYARAVTERAGVELIERWRPPPGDLFAPPARLRVRPGGFGLLGPSDALF
                     LEAAREFGADAIFTGAGGDNIFGYITSAGPVLDALRFAGPLAAWRAAGDLARLTRDNR
                     WKAIFYAARRMLRTPAPWPVEHSLLSDRYADAMPAHPWTEGADRVPAGQRTYGLALLL
                     MQSFVNAYDRALALPKIAPLLSQPLVELGLGVPSWQWSEGGKDRALARAAFADAVPPE
                     VLARRSKGRILSVFLPVFARERAALRPFLLDGWLAGAGILDSDAVAAMLSPGEGDPLS
                     IMRVLEVVDIERWARSVA"
     gene            complement(13767..15746)
                     /locus_tag="VN034_03100"
     CDS             complement(13767..15746)
                     /locus_tag="VN034_03100"
                     /inference="COORDINATES: protein motif:HMM:NF033523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Atxe2 family lasso peptide isopeptidase"
                     /protein_id="HWV59614.1"
                     /translation="MVRIADLLAAAASAAATVVPAQTAERAPVTTRDLVEVAEISAPT
                     ISPDGRRVAYRVSHPSVEANASRLRWHIAAIDGGGEIVALDGGTERPDASGVPVEAAP
                     VWDRDSRGLRFLALREGVAAVWHWREGQPLVREIVDEADILDFGLSADGKGVRYSVGA
                     TRAAIASAERSAYRDGVVVDASINLNQPVAGGVIEDGQRIMRRISSPWFDNQRILWDS
                     PRADKIYVPADPAELPAAPSPLQGLSSRGADGSIAEISGAKAEQRLRVRRTDGREVPC
                     TAAPCRSGGLLALSWIPGRDALVLFAKDRSARETLWRWDIGARRATRLTVTSGALRVP
                     GRPPRCAVGADALYCADARPTTPPRLVRIAWDGEERILAEPNQALGARISAKAEPMAW
                     PGGFTGFFLTPAGTSGPLPVVVQYYMCDGFLKGGTGDEIPMLPLVEHGIAILCIDAIR
                     APRAAGMEGSYDLALKTISNALDGLAADGRIDPARVGIGGLSFGSSVALWAIRKSKRF
                     AAATVASGQVSPHYYWINAHPERGFTKVLDEYWNLRDPESDPERWKDISPVWDTEVFD
                     TPLLMQLPESEIQSNVEFHARLKRAGKPAEMVVFADELHIKYQPAHKRASYERNLDWY
                     RFWLKHEEDAAPEKAAQYRRWRALRAGQSLPAPAR"
     gene            complement(15740..18664)
                     /locus_tag="VN034_03105"
     CDS             complement(15740..18664)
                     /locus_tag="VN034_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59615.1"
                     /translation="MKRLYLAALLATGSGLAIGAPSAAAQTGQTDYDIAAQGLADAIR
                     EYSRVSGRDVIAASSLVEDRRSSGVKGRLSPEAALARMLAGTGLMAELVDGALVLRAQ
                     ENSALGDAKPAGNPDAAIIVTGTRIRGTGPVGSPVTVIDRAELDRSGRATLADFIQTI
                     PQNFSGGPTEANIGMSARGNAISNIGLGTGINLRGLGPGATLTLFDGARPALGGASGA
                     FTDLSLVPSAIIERVEILTDGASAIYGSDAIAGVVNIRFRNRFEGAETRLRVGTADGD
                     YGEILAGQIVGTKWAGGSLVVAGEYQRRGALPSTQRAFATEDLRPFGGPDLRSNYNVP
                     GTIVAANGQRFLIPPGQDGTGLTRADLIATSDFNRGDARRNIDILPRQRSASGYASVE
                     QRLAGDTMLFARALYADRRFAARRRLNGTVPIMVTSANPYYVDPIGTGQPVTVYYDPS
                     ADFGPEGVRGRVKAFNASAGARGRFGSWEIELAGGYGVQREHYDGVNLIHFLRLPRAG
                     TASTISTALNPFGDGAVNDPGLIDRLRGSLAVDTRFTVWTGALRADGTLFELPAGEVK
                     LAFGAEYRRDRLTYVQTSDLAFDTPRTDGIPGLPDKRIVTAIYCELSLPVFDAGAHFP
                     GKLQLSAAGRYEDYSDVGDTANPKLGARWTPLPGLALRASWGRSFRAPFFDELVGTAN
                     ARYQTLRVADPQSPTGQSVVLALFGFRPDLGPERATSWTAGIDFEPEFLPGLKASATW
                     FDIAYRGRIASGSAEFRNFLVRRDLYAPLVTENPEFGVVSAYFANPSFSNPIGAAPGD
                     VRALLDVRTRNLSSSHVRGLDFDLGYTRDLGEGRVTLALGGSRLFEIDNRLVAAAAAN
                     NVVGTLGNPVKLRLRGYAGFALGSFDGGIGVHHVGAYRNRTVTPEERVKSFTTVDMQL
                     GARIGDPGGRALRLALSINNIFDSDPPYARFQAIGSAVGYDPEQASPVGRTLALQAVI
                     TW"
     gene            complement(18734..>18963)
                     /locus_tag="VN034_03110"
     CDS             complement(18734..>18963)
                     /locus_tag="VN034_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWV59616.1"
                     /translation="SKLDADDLPLGAIVDRANRVNARKIGLADPALAGLRVSGRFEID
                     DGDALARKLAAALDLGIEAGAAGPVLKRPRK"
BASE COUNT         3076 a         6240 c         6418 g         3229 t
ORIGIN      
        1 tctccccgcc ttcgcggggg cgacggaggg taatatgacc accgccaaac tcttcatcca
       61 cggcgttccc gacagcccgg cgatctggcg gcccttgctt gccgcgctcg agcttggcga
      121 cacgccggtc gcggtccccg cgctgcccgg cttcaccgcc ccgctgcccc cgggtttccc
      181 cgcgaccaag gaggcctatg ccgactgggc ggtgggcgag gccgaagcct tggttgctgc
      241 gcacggcacg atcgacatcg tcggccacga ctggggggcg ttgatcgcgc agcgcgtcgc
      301 gatgctgcgc cccgacctga tccgcagctg ggcgatctcg aacgcggtga tcgaccccga
      361 ctatcgcggg caccgcatcg cgcgcatctg gaacacgccg atcctcggtg aaatcttcat
      421 ggcgctgagc aaggccgaca agctcgccga ggggctcgcg gcgcagggca tgcccgccga
      481 catcgcgcgc gaagaggccg ggcagtggaa gcacaaggac aagcgccgcg cgatcctcaa
      541 actctatcgc tcggcgaagg ggctgagctt cgcgcacgac tgggcgctgg acattccgaa
      601 actgcccgcg aacggcgcgc tgatctgggg tgagggcgat ccctatgtcg aactctcggt
      661 cgcgcgacgc tatgcaacga acaccggcac gctgctgacg gtgatcgaag gggccgggca
      721 ttgggcgatc gctgaacgcc ccgtcgaggt cgccgccgcg ctccatgcct tctggaacgg
      781 gctgtaaagc gaggataata ttatttcatc gttttgcacg attattgtga cgcaataaat
      841 cgattggaac gccggaacga tccggcgcat tgagtggata tcgcgaaagg gcgtcccatg
      901 gacgatggca aatctccccc tgaactgcct cgtccatccc gttccgaagc agatggcccg
      961 gacctccccc ctcccgaccg gaccactggc gccgtgccca tcatcgcgac cgcgacgatg
     1021 ggtgcggcgg accagcgatt tttcgacgcg ctgcgtgccg cgcatttccc gcccgaacgc
     1081 aatcatctcg ccgcgcatat caccctcttt caccaattgc cgccgtcgtg cctcgacgaa
     1141 ctcgatcgcc tgttccgccg catcgccgcc gacacgccgc cgcccgccgc aatgctgcgc
     1201 gaggtctatt cgctggggca gggggtcgcc tttcgtatcg agagtcccga gctgctcgat
     1261 gtacgcgcgc gcatcgccga ccatttcgcc ggcatgctga ccgcgcagga ccggggagtg
     1321 ccgcggctcc acatcaccgt gcagaacaag gtcgccgcgg ccgaggcgcg cgcgctgctc
     1381 gcggcgctgg catgtgattt ccgcccgcgt ccgcttacga tcaccggact cgcggcgcat
     1441 cgctatcgcg gcggcccgtg ggaacccgcc ttcgcgcgca gttttcgggc gatgcgcaga
     1501 tgatattgac cggcgcgacg agcctgccta taggcgctgc tcgcccgatg cggcggcctt
     1561 tcggggcgga gtagctcagc aggttagagc agcggaatca taatccgcgt gtcgggggtt
     1621 cgagtccctc ctccgctacc agagccaatt ttcccataaa gtcccattcc gtttcattgc
     1681 gtcccgttat agcaatgaaa tcaaatattt ctggcgccct ccttatcccc gctgcgtccg
     1741 gtttcgtccc gcaaggtccc actgcgggaa tttgatcctg gggtagattt tgggcatttt
     1801 ccccggacta ccccggcagg aaaatacccc aatggctctc aaagacctcg agatcaaata
     1861 tgcggcgcgc cgctcgcgcg actacaagct cttcgatggc gaggggcttt acgtgctcgt
     1921 cacgccccgc ggatcgaagc tctggcgtct caaatatttc ttcgacggca aggaaaagac
     1981 gctgagcctc ggcaaatatc cggcgatttc ggccgccgcc gcgcggcaga tgcggcgcga
     2041 ggccaaaaag ctcgtcgccg atggaatcga ccccggcgag cacaggcggt cgaacaaggg
     2101 caggctgccg ccgcagaccc ttttcgagcc gatcgcgcgt gcctggcacg cgaatcgctg
     2161 cgaggcgctc gatgccgccc atgcgtcgcg cgtgatgagc cggctggagc gcgatgtctt
     2221 cccggtgttg ggacaacgcg acatcaagtc gatcacagcc cccgaaatac tcgaggtcgt
     2281 gcgcgcggtc gaggcgcgcg gtgccctcga cgtcagccgg cgggtgaagc agagcatcgg
     2341 ccaagtcttt cgcttcgcga tcgcgagcgg ctgggcgacc aacgatccca cgaccagcat
     2401 caacgatgcg ctccggccca ggccgcgcgt caagcatatg ccgaaggtgc cgctcgccga
     2461 gctccccgaa ctggtcgccg cgatccggag ctacgacggc gaggccgacg cgcgccgccg
     2521 cgcgacgacg cgcgacgcga tgatgttcac cttgctcacc tgggcgcgaa cgagcgagac
     2581 gcgcttcgcg tcgtgggacg agatcgatga cctcgacggc gagatgccgc tgtggcgcct
     2641 gccggccgaa cgcatgaaga tgcaccgcga gcatgtggtg ccgctctcgt cgcaggtcgt
     2701 cgagctcctg ctgcgccggc gggcgcgggg cggacattat atattcgaag gcgacaagcc
     2761 cggacatccg atctcggaga atacgatgat cttcgcctgt tatcgcatgg gttatcgcgg
     2821 ccggcagacc gtccatggct tcagggggct ggcatccacc tgggccaatg aggccgaatg
     2881 cttcaagcct gattggatcg aaatggcgct tgcccacgcc gatgaggatg aagtgcgggg
     2941 ggcttataat agcgcgctgt atatgtcgcc ccgacggcga atgctccagc gctgggccga
     3001 cgagatctac caagagtccc cgcttgttac ccctcgcgcc caaatgcgct cctcgttgcg
     3061 ccaagtgtca aaggccactc cgggcacacg cggcgaagca aggtcagagc gtctttcgtc
     3121 cgagcgcctg ccgttctggc agcgccccga cgggcggcgg ggtcttccaa agccgcgctt
     3181 cggctcttaa gatccggaga gggggccgct tcgggtctgc acgcgacggg gactggatgg
     3241 gcgtaacctt tgaccgggcg gttcggatcg ccggctacag gccatcggtg ttcaaggacg
     3301 cgctgcgcgg atatatgcgc accctcgatc ccgcatgtct gatcgatatc aagagtgtct
     3361 ttccgcatcg gcgcgacggc gcgatcgtct atgaggaatg cctcgaccgg gggctgatcg
     3421 atccggtgac gcaccaggtg accgagcgcg gaatggccgt cgcgcgcgcc aaggtgaccg
     3481 cgcgaacgcc gctcatcaag gcgagggcgg tcctggacga actgctgaac cgcatcgacc
     3541 ggttcaatgc ggaccccgaa gctgtatccc gcgtcgacga gatatggctc ttcggaagca
     3601 tgatgcgcga agaagctacg gtcggcgaca ttgaccttgc catttgccgg tcgcgtgacg
     3661 cgcggttcgc ggacgatctc gacggtcagg tcgagcaggc gaagcggctg atccagggat
     3721 atccggacgc gccgcagcgc tgggcctggc cctgggaccg gatcgactgg ctctatcggc
     3781 ggagcatatt cgggccgcgg cggcatcctc tcctcgctgg cgcgcaagaa ggaacgacgg
     3841 atctccaatt gctcgccgtg ccttgccggt tgatctacga ccgggcgcgc ggcgggcgag
     3901 taagcgaccc ggttctgcct cgtcacccgg aatcgccggg gcgcgcaaat acgctgttcg
     3961 ctcccgatga attgcccgat ctgacgccag gcgagcttcg cccgatggat gcgcggtgga
     4021 tcgccggctt caagccctgg ggcgcggtgt cgccatacga tatctttcgc ggttggacgg
     4081 atgagggtga gcggcttttt ggtagctacc cggagcattt gcggatcgtt gccgacggcc
     4141 atgacctccg gggttttccc tggataccga agccggtgag caagggcggg ctcgatggcc
     4201 gcaatgcaat cgccatcgtc aacgcagccg actggtgcgg cacgtcaatc accttgcgcc
     4261 gcgcgatcga gggcaggccg ggcagtcggg ggttgcacgc gagctttacc gacctcgcat
     4321 tatatcggcg ccgcaagcat gtggagattt cgactttgtt cgatatggta tcggcggtgt
     4381 cgatcatcct cgcggtggat gccgagcgca ttctacggcg ggcgacggaa agcggcgaac
     4441 ctcgggaggt cacaatccgg atttcttcgg acgatctgcc tgaagacatg cgcaggcatt
     4501 tcgcccagga aattgccgac atgctggccc ggcgagccgt tgcgatagag ccggcgggcg
     4561 aaaccgcctc ggttcatatc cagctcgcct agcgaacgga tcgcggttgt gcttcgcacc
     4621 tcccttgcct tgtggcagag ctccgccaaa gggtgagatt cgggagtggc gatgacggcg
     4681 agaggcaagg gcggccgagc gggcagcacg atcaatacgc ggtcgccgcg gatgaaatat
     4741 ttcaggcttc ttggctggtc tccctacaat agtccgagcg aatatatcgt gcatctgtgc
     4801 aaggaactcg gacgcctcgg catcgcgatc gaagatatcg agacgcacag ctcgccgccc
     4861 aacggcgaag aagtcttgtc cgaactggtt gcttggctcg ccgagcatcc aggtgccaac
     4921 tattccgcac tcgtcgactg gaaggttctc gacatagcga atctcgctga tcggatgcgc
     4981 tcgaatccgg atgccaagcc gcgcatcgag ataaccgccg aaattacgaa aatgcgcaac
     5041 ctgtcggttc gctggtatcc ggcgggctgg cacgacgcga tgcccgattg gagcggccgc
     5101 gacccgttca acatgatcgg acataaggtc tgggataccc tgcccgattt ccgctggttc
     5161 cgtggcgacc agcctgcgac gttcgacggc cgcgcgacca acgaactgat gtttattcgc
     5221 ggtgtcggca accttcaccc cgagccgcgc aacgaatacg gcttttatgc gaagttcaat
     5281 cacgaccttg tcgacggcat gctcatcgcc gcagtgcgcg cagcctatga gcgactgatc
     5341 gggcagcttt cccgtagctt tgacgtggag gtcctgaatg cattcgactt cgaggttcgc
     5401 gatgaggaaa gcgatgacga taattccggc ggtcgttctg gccgacgtgt cgtcggctgg
     5461 gcgctgctcg atgcccgcga acttcgagcg cgccgggagc gggaggccga ggaagcgcgc
     5521 gaagcccaag gacgcgcgga tgttgcgcag atcgcggcga ggcataattg taccttcgcc
     5581 gaattcttgc aggcgcttga tgacgccgtc cattcaaagt cgaataattg cgcagccagc
     5641 gagcaagtct atcgcgtcgc cgcgaaatcg ctccggaagg cgggcgccga tatcaacgca
     5701 ggcgatgtcc ggcgtttggt tcggataatc ggcgacttca tgtccgaaga gcttcctgct
     5761 tccttgagat gcgatgagtc ggccgcggcc ggctagcgcc gacggatatt ggcgggatgg
     5821 agaagcggtg agcgatccgg gtggagccgc gcaccctccg cgcacggcac gaacgattta
     5881 ttcaaaccac gggccagcga acgggtcttc gagatgacgg cgcgcctttc ggacgagacg
     5941 ctgcacccgc aggcggtagg atgtcgcgtc gacccgccag cgcggcgaag cgaccgcgag
     6001 agggaaaaga tgccgcgcca tgtcctgctg ggatgctccc gcgagcaggg cgtcggcgac
     6061 acgcagctcg agaatccatt gtgcggcgcg gcgctcgcgc ggcatcaggg cggccggcaa
     6121 ctggcccttt tccgcgatca tagcgagctg cctcagcgcc gcgagcttcg gtgtgagcga
     6181 ctgcaggccg gtcaggcgat gctcgatcag aagcggaccg ccgagcaacg tgccgtcgtg
     6241 aaggtcgaga cggatcgccc aatgaccgtc gctcagcaac caatgctcgg cgcgcgccgt
     6301 cacttcgacc gaaaccagcg gcgcgacggt gagaatgtcg agcatgtcgc cggtagtgcc
     6361 gctattcccg cctgccgccg ggcggccgtc aagcacgccc ggatcggtgg tcacgctcca
     6421 gatcggtcgg gcgcgcggtg ccggtatcgc cgggtcgatc cacgcgacca gtcccatccg
     6481 cgccgatgcg tcgcctggta gccggtcgcg tctgttccag tcacgacggt aggcggggtt
     6541 gcgacggagc cactcccagg caaagaatct ccgctcgcac agcagcagtt ccgcataggc
     6601 ctcggccgaa cgccagtcgg cggctggcgc gcccttgctc acgtcccata tccggaatgg
     6661 cggaggaggc acggcatggt gcgtcgatcg aatccggctt cgaccggctc agatacgcag
     6721 cggaaccgtc agcgccagcc gcgccggcga agcgcggctg cagataatcg acgcgggctg
     6781 gcggacggca agaccgaagg cgccgcgtcc tctctcgcgg ctcgcccctg gccgacgcgg
     6841 cacgcggcat ctattcgccg ccttcggcaa aataggcgcg caaccggtgc gcggtatcgg
     6901 gggcgatctt catgaagctg cgacgcccgt cgcccgggtc gggaacccgg catagcaggc
     6961 cggcgtcgca gagccgctgc gcgaggcgca tcgcgctgct ttccgggatg gccgcggcga
     7021 cgcatagcga cgacatcgac aactgctttc cttcgcactc gtggacgaag aggtcgagca
     7081 acatgtccca cgccggctcg ccgaacaggt ccgccgcgcc gagcaattgg cgccggagga
     7141 tcttgcgacg cagcgtcgcc ttcgcgttgc cgagaatgcg atcattggtg tcgccgggcg
     7201 gttgggggct cgcgcttcgc agcgccaggc tcttcggacg gtccgcggcg acggttttcg
     7261 tttttccgct ccgcaaatga tgcgttcgtg tggcggtgac gacgatatga tcgagaagtt
     7321 cgatatcgag ggaccgtccg acccgctcga gcaggtcggt cgcctggata tcggcatcgc
     7381 tcggtttcgg atcgccgctc gggtgattgt ggatgagaat gatggcggcg gcattcagtt
     7441 cgagcgcgcg tcccaatatc gttcgcggat agatggacat atgcgcgagc gtcccatgtt
     7501 gcagctgctc gtcggcaagg agcccccgcg ccggatcgag aaacaggacg cggagccgtt
     7561 cttcggggag cgatcccatc gacacggtca gatagcgacg cagcgcggcg tcgagcggat
     7621 cgatccggcg ggtcgcgaaa tcggcgcgca tcgtctcgtg cagcgcgtcg cgtccgctcg
     7681 caagcaatgc gatgacggcg gggcgccggc cgatgacccg ggcatgcgcc tcgggactct
     7741 gcgaccacat ccgcgacagg ctgcgatact ccccgagcag cgcggcggct atggcttcgg
     7801 catgctcggg ttcgaccgcc gccacgaagc gtgccaggac ggatcgctgc cgcaggtccg
     7861 cagccgcttg cggtgccgcc aggttccgag gaccagcagc ggcgggagaa gccaggacgc
     7921 aaatacctgc atcgtcagat aggcagccgg atgcaccgag aggtccgccg ctcggctgaa
     7981 atgggcgaga agcggcagga gctgcagcgc cgccgccgcc agcggccaga agcggtgcgc
     8041 cgtcagcgcc agcgccatcg tcgcgatcgc cgcgaagaga tcgatcgcga gatggccgct
     8101 gtcgagcgcg tcgagacggg ccggaacgaa gagatggagc gcctgatccg cgatcagcat
     8161 cgcgatcagg atcatcgcca tcgcccgctc gggggcgccg cctttcctca gtgcgtacaa
     8221 tgtcgcgagc gcgagcaggg ttacaaagct tgcaatccgc agcatcttta ccggaaacca
     8281 tgaccgtgcg cctcggtcaa ccggctaggc cgtgcgctgc gcgtcggcgg attgtgcgac
     8341 ggccggacac tcgtggagat cgagacccga ccgctcgcgc gcgataccgc tcaactcgcc
     8401 atggacgcgc agcatcccgc cgctggcgtc gatcaacgct cgttgcgcat cggcgaggcg
     8461 caggatggtt ccctgttccc tggctggctc ggcgcccccc atgtcacggc gcgcggtcac
     8521 gacggtcgtc atcagcgacg aaacggcgat cagcgcgtcg tcgatgcgcg tctcggcgaa
     8581 gggcacctgc tgctgcagct tcctggcgac gaatgctatt gtgtcggaca tgatctctcc
     8641 tccgccggaa aggcgttccg gccattgcga tggactggga gaagcgtcag ttcgagatca
     8701 gctgcgtcaa tgcctgcgcg atcccgagcc cgccgaggag agcgaagatc accgtcgcga
     8761 gcatgcccgc cacgatcgcc agcctcgtcg tcgagccatg atcatgactc ccgagcttcc
     8821 cgaacggcag gccttcgccc agaaacccgt ccctcgcccg gctgagaaca tcggttcgag
     8881 ccggctcgga tgggtcgccg tccgggaagt cggatggcac gagcgccggg ggtggaggaa
     8941 tatgcaccgg atcatatgtg atccggtcat atatctgctt gagccgtgcg tagcggcgcg
     9001 cggtgtcgtc gcgatcgatc gcgccgagga tggtgcgtgc cgccgtgatg cgctggtcga
     9061 ccgtcggctt cgcaatgccc agctgccggg cgatctgctt cgatgtcttg cgctcgagca
     9121 gcagatcgag acagtcgcgc tgcttttcgg tcagccgatc ccattgcgcc cgatcggcgg
     9181 cggtagaaga ttgcgaccct tccatcgcga cagtttcatg acaaaaggtc ctgcgcacaa
     9241 gcctttatcg ttcgccattg cagccaggag ggcggcccgg atgtgcattt ccccacgcgg
     9301 gaccgggctt gaccgtcgcc gctcgcatgc ccgtcgcgcg gcccggcgtt cctccgcatt
     9361 cgacgttcgt gatcgcgaca ccgcgtgaca acaactctca aattgagagt aacctgcgaa
     9421 atataatgga aatattgttc gggccggata gccttggccg aacgaatcgc cgcccgacca
     9481 tcggcgatgc atcaccgccg tcccgccgcc tggttgagag ggcccgcggc cggcaccgga
     9541 tgggagcaac gccggaccgg caggcttgag gacgccgccc ggcttgccag ggtcgtcatg
     9601 tcgacacgcg atgccgcgcc gcgttgtggc cggctcgatg ccgttcgcgc cgcagcctcg
     9661 cggcctttga tcactggcgc ccgccgcctg cgcccgcccg ccgcgtgcgg ccggagggcg
     9721 aactgcgggg tcggggcagg gcgggccctt cgtcggcatg tctccgcggc ccgctccccg
     9781 gtcccgcaca tccaatgcag caggggcttt tgatgctcgt cgtcgaccag tcgacgcgcg
     9841 cgcgcgagca tgctgtcctc cgggcgatgt tcgcggcgcg caagcgcgtt ttcgtcgacc
     9901 tcctcaaatg ggacctgccg gtcctcgccg gccgcttcga ggtcgaccat cgcgacggcc
     9961 ccgacgcgac ctatctgatc gtcgccgggc ccgaaggcga gcaccgcgcg tccgcccgtc
    10021 tgctacctgt ctgcccggca tgcttcaccc gggcggaagg atgcctgttc caaaggggcg
    10081 accgcgcgtc ggggatcgcc gaggtcacgc atttctgcgt ctcgcccgat atcgacggcg
    10141 acgcgcggcg gcacgcgcga gaccagttgc tccacggtct cgtcgaccac gcgctcgcgc
    10201 ggcggattca ccgcttggtc ggcatcgcgg aagaggcgtg ggcccggcgg gccgaggggt
    10261 tcggatgggc gtgccgccgc gtcggcccgg ttcgcgcgcc cttcggccgg aatatggtga
    10321 cggtggtggt cgacatcgac cggacgaccc ccgagcggct cgtcgctgcg ggcatcgtgc
    10381 ggtcgtcggc gccaaggcag gccctctgat gagcccgggc gccacgatgg aacgcgtcta
    10441 tcgcgaactc aaggcgcgcg cgatgcgcgg agacttcgcg ccgggcgagc gtctcgatcc
    10501 ggcggtgctc gcgcgcgatc tcggcgcgag cgtcacgccc ctgcgcgacg cgctccaccg
    10561 cctgtcgggc gagcgcttca tcgacagctg gcaccaggaa ggctttcgtc agccgattct
    10621 ggccgaggcc gatctccacg atctttatga atgggcggcc gcgctgctga cgctcgcgct
    10681 cgccgggcgg aagccggacc cctcgttgcc cgatgcggcg ccaaacggga cccgcgagcc
    10741 cgattacccg gccgaagtcg ccggtctctt ccgcgcgatc gcctcgcaaa gcggcaaccg
    10801 cgaactccgc ttcgccgtcg tcaacctgat cgagcgttcg catgtcttcc gcgccgccga
    10861 actccatgtc gatcccggct gcagcgccgc gatcgccgcg atggaagagg attttcgctt
    10921 ccgtcgctgg agcgcgctgc gcagcaaggt cacgcgcttc catcaccgac gcgcggcgtt
    10981 ggcaggacgc gtcgtcgcgg cgatgcggcc gcgcgaacag ccgctcggat aggtaccgga
    11041 atatattcag aatatattcg gactatattc gacatatatt ccaattataa aattcctata
    11101 tgagatcagg ggcttactct tcccgattgc cgcaatcccg cggtatggaa gaggagggtc
    11161 caatggaccg tcatgacatc gccgaagacg gcatcgaaga actgggcgtc gcgagcgtcg
    11221 aaacgctggg gtcggctccg ccgttccccg aagaggagac gctgagcccg gttccgatcg
    11281 gcggcggcat caccgctgaa tgatcggttt gcggacgcgg cgccccgccg cgtccgcttc
    11341 ccgccatgga tttccatctc gcaccccatg tcagctacgg gctcgtcggc cgccgcgcgg
    11401 tcatgctcga tctcttggcc gatcgctatc tgctcctcgg ccccgaggtg gccgacgtgc
    11461 tcactgcgct tggccgatgc aggtctgtct tttcggtcgc cgtcctagag aaactgctcg
    11521 cgcgcggact gctgttgccc gggccgggcg cgacggtcgg gccggtcgga caaccttgcc
    11581 ccctcggtag cgcgctcgaa gccgatcttt ctggcggagg cgtttcggca tttgaagcgc
    11641 ttcggcgcag catcggcgcc cttataacga tgcgcgccct tggactcgct ccacttattg
    11701 cgcgctcgcg cacggtgcga gcgcgcgctg cgcgccgcac ggaactcgtc gaggatccag
    11761 accgcgccgc cttcttcgct cgcggcttcg cggaagcgcg aatatcggtg ccgatgccgc
    11821 ggctctgcgt ccccgacagt ctcgcgctcg caggcagcct ctgggcgcgc ggcctggccg
    11881 cagaagtcta tttcggcgtg caactggatc cgctgctcgc gcacgcctgg gtccagcagg
    11941 gcccgctcgt cctgtccgac ccgctcaaca tcgcggccga ctaccgtccg gtgttcagac
    12001 tgtgagcatt cgtctcgcga tcatcgaagc cccgaagact catacgggct cgccgcggga
    12061 ccctgcaccg cttgcgtggg tgggcgcggc gcgcgtcgtg ggcgaatgga caacgccatg
    12121 gtgggcgggg gagggcgtcc ttctcgtcgg ctgggctttc cgccgcgacg gatttggtgc
    12181 ctgcacccga ttgtcgagcg aggaagtcga gcggctggtc gcgagcgagg gtcgatgggc
    12241 gctagcggct ctctggggac agtttatcct cgtttgggcc gaccccgacg gccacatacg
    12301 gctgttgcgt tcgcccgtcg acggaccgcc tatctatcac aatgtcgggg agcgcggagc
    12361 ctgtgccttt accgacctag cgctggcgcg ttcgctcggc ttcgcgctgt cgggccccga
    12421 tccgatggcg gtcgatgctg cgcttcgttt tccctttcta cgggcggcga gaaccgaact
    12481 tgccggggtc cgcgagattc ttcccggcga gatcgccgag cttggcgatg gccaccggca
    12541 gcctggaagc tggtcgccat gggattacgc ggtccggccg ccccggcgca tcgcgcgcga
    12601 ggaccttcgc acgatggtgc aggcggtgac cggtgcatgg agcggtcgtt tcgggcgcgt
    12661 ccagctcgaa ctctcgggcg gcctcgacag ctcgatcgtc gccatgtgcc tagccggacg
    12721 ccgcgccgcc tggcgcgcga tcacgctcgc gacgcgcgag cccgatggag atgaaaggcc
    12781 ttatgcccgc gcggtgacgg aacgtgccgg ggtcgagctt atcgagcgat ggcgcccgcc
    12841 gccgggcgat ctcttcgcgc cgccagcgcg gctgcgcgtc cgtccaggcg gattcggcct
    12901 gctcggcccg agcgatgcgc tgtttctgga agcggctcgc gaatttggag ccgacgcgat
    12961 cttcaccggt gccgggggcg acaatatctt cggctatatc acatcggctg gccccgttct
    13021 cgacgcgctt cgcttcgccg gtccgcttgc ggcatggcgc gccgcgggcg atctcgcgcg
    13081 gctcacccgc gataatcgct ggaaggcgat attctatgcg gcgcggcgga tgctgcgaac
    13141 gcccgcgcct tggccggtcg agcatagtct gctgtcggac cgctacgccg atgcgatgcc
    13201 cgcgcatccc tggacggaag gcgccgaccg cgtgccggcg gggcagcgga cctacgggct
    13261 cgcactcctg ctcatgcaga gcttcgtcaa tgcttatgat cgcgccctcg cattgccgaa
    13321 gatcgctccc ttgctctcgc agcctcttgt cgaactcggg ctcggcgttc cgagctggca
    13381 gtggagcgag ggcgggaagg atcgcgcgct tgcccgcgcc gccttcgccg acgcggttcc
    13441 gcccgaggtc ctcgcgcggc gcagcaaggg gcgtatcctc tcggtgttcc tccccgtttt
    13501 tgcgcgcgag cgggcggcgt tgcgaccctt cctgctcgat ggctggcttg ccggcgcggg
    13561 tatcctcgat tccgacgcgg tcgccgcgat gctctcgccc ggcgaggggg acccgctcag
    13621 catcatgcgc gttctcgagg tcgtcgatat cgagcgctgg gcgcgatcgg tagcctgagc
    13681 cggcacgccg tgccatggcc gaacgaaacc gggccattgg caggccgtgc cgggcgcgac
    13741 cggggaggtc acgcccggca ccaaccctag cgcgcggggg ctggcaagga ctgccccgcg
    13801 cgaagcgcac gccagcggcg atattgcgcg gccttctcgg gcgccgcatc ctcttcgtgc
    13861 ttgagccaga agcgatacca gtcgaggttg cgctcatatg aggcccgctt gtgtgcgggc
    13921 tgatatttga tgtgcagttc gtcggcgaag acgaccatct cggccggctt gcctgcgcgc
    13981 ttgaggcgcg catggaattc gacgttcgac tggatttcgg attcgggaag ctgcatcagc
    14041 agcggcgtat cgaatacctc ggtgtcccag accggcgaaa tatccttcca tcgctccgga
    14101 tcgctctcgg gatcccgcag gttccaatat tcgtcgagca ctttggtgaa accgcgctcg
    14161 ggatgcgcat tgatccagta ataatgtggc gagacctggc ccgaggcgac ggtcgcggcg
    14221 gcgaagcgct tcgatttacg gattgcccag agcgccaccg aggacccgaa gctcagtccg
    14281 ccgattccga cccgagcagg gtctatgcga ccatcggcag cgaggccgtc gagcgcattg
    14341 ctgatcgttt tcagtgcaag atcgtacgat ccttccattc ctgccgctcg cggggcccgg
    14401 atcgcgtcga tacagagaat ggcaatgcca tgttcgacga gcggcagcat cggaatctcg
    14461 tcgcccgtgc cgcctttgag aaagccgtcg cacatataat attggacgac gaccggcagc
    14521 ggaccggacg ttccggccgg agtcaggaag aagccggtga atccgccggg ccaggccatc
    14581 ggctcggcct tcgccgatat gcgcgcgccg agcgcctggt tcggctcggc gaggatgcgt
    14641 tcttcgccgt cccaggcgat gcggacaagt cggggagggg tcgtcggccg cgcatccgcg
    14701 caatagaggg catccgcgcc cacggcgcag cgcggcggcc gaccggggac gcgcagggcg
    14761 ccgctcgtga ccgtgagccg tgttgcgcgc cgcgctccga tatcccaccg ccagagggtt
    14821 tcgcgcgcgc tgcgatcctt tgcaaagagg acgagcgcgt cgcggcccgg aatccagctg
    14881 agcgcgagca gtccgcccga gcggcagggg gcggccgtgc atgggacttc gcgcccgtcc
    14941 gtgcgccgga cgcgaagccg ttgctcggcc ttcgcgccag agatttcggc gatgctgccg
    15001 tccgcaccac ggctcgagag gccttgcagg ggcgagggcg ccgccggaag ctcggccgga
    15061 tcggccggta catatatctt gtcggcccgc gggctgtccc agagtatccg ctggttgtcg
    15121 aaccacggtg acgaaatgcg gcgcatgatg cgctggccat cctcgatcac gccgcccgcg
    15181 accggctggt tgaggttgat gctcgcgtcg acgacgaccc cgtcgcgata ggccgagcgc
    15241 tcggcggaag cgatcgccgc gcgcgtcgcg ccgacgctat acctgacgcc cttgccgtcg
    15301 gccgacaggc caaaatcgag aatatcggcc tcatcgacga tctcgcggac gagtggttgg
    15361 ccctcgcgcc aatgccatac cgcggcgacg ccttcccgga gcgcgagaaa gcgcaggccg
    15421 cggctgtcgc gatcccagac cggcgcggct tcgacgggaa ccccgctcgc atcggggcgc
    15481 tcggtgccac cgtcgagtgc aacgatttcg ccacccccgt cgatcgcagc gatatgccag
    15541 cgcagccgcg acgcattggc ctcgaccgac ggatggctga cgcgataggc gacccggcgt
    15601 ccgtcgggcg aaattgtcgg cgcgctgatc tcggcgacct cgacgaggtc gcgcgtcgtg
    15661 accggggcgc gttcggccgt ctgggcggga acgacggtcg cggcggcgct ggccgcggcc
    15721 gcgagcagat cggcaatcct caccatgtga tcaccgcctg cagtgcgagc gtacggccaa
    15781 ccgggctcgc ctgctccggg tcgtagccga ccgccgagcc gattgcctgg aaacgagcgt
    15841 aaggtggatc gctgtcgaag atattgttga tgctgagcgc gaggcgcagc gcgcggccgc
    15901 cgggatctcc gatccgcgcg ccgagctgca tgtcgaccgt ggtgaaactt ttcacccgct
    15961 cttcgggcgt gaccgtgcgg ttgcgatagg cgccgacatg atggacaccg atcccgccgt
    16021 cgaagctgcc gagtgcaaaa ccagcatatc cccgcagccg gagtttcaca ggattgccga
    16081 gcgtcccgac gacattgttc gccgccgccg ctgcgacgag acggttgtcg atttcgaaga
    16141 gtcggctgcc tccgagcgcg agcgtcaccc ggccttcgcc gaggtcgcgg gtgtagccga
    16201 gatcgaaatc gagcccgcgg acgtgcgacg aggagaggtt gcgcgtgcgc acatcgagga
    16261 gcgcgcgcac gtcgccgggc gcggcgccga tcgggttgga gaagctcgga ttggcgaaat
    16321 aggcggagac gaccccgaat tccggattct cggtcacgag cggcgcgtag aggtcgcgcc
    16381 ggacgaggaa attgcggaac tcggccgagc ccgatgcgat gcgcccccga taggcgatat
    16441 cgaaccaggt cgccgaggcc ttgaggccgg gaaggaattc gggctcgaag tcgatccccg
    16501 ccgtccagct tgttgcccgt tcgggtccga gatcgggccg gaagccgaac agcgcgagaa
    16561 ccaccgactg gccggttggc gactgcgggt cggcgacgcg aagcgtctgg tagcgcgcgt
    16621 tcgcggtgcc gacgagttcg tcgaagaatg gggcgcggaa ggagcgcccc caggaggcgc
    16681 gcagggcaag tcccggaagc ggtgtccacc gcgcgccgag cttgggattc gcggtgtcgc
    16741 cgacgtcgga ataatcctcg tagcggcccg cggcggaaag ctggagcttt ccggggaaat
    16801 gcgctcccgc atcgaagacg ggaagcgata gttcgcagta gatggcggtc acgatgcgct
    16861 tgtcggggag tcccggaatg ccgtccgtgc ggggagtgtc gaacgcgagg tcgcttgtct
    16921 gcacataggt gagccggtcg cggcggtatt cggcgccgaa ggcgagcttc acctctcccg
    16981 ccggcagctc gaataaagtc ccgtcggctc gcagcgcccc ggtccagacc gtgaagcgcg
    17041 tgtcgaccgc gaggctgccg cgcagccggt cgatcaaccc cggatcgttg accgcgccat
    17101 cgccgaacgg attgagcgcg gtcgagatag tcgaggcggt gcccgcgcgc ggcaaacgca
    17161 ggaaatggat cagattgacg ccatcataat gttcgcgctg cacgccatag ccgcccgcga
    17221 gctcgatctc ccagctgccg aaccggcccc gtgcgccagc gctcgcgttg aaggccttga
    17281 cccggccgcg gacgccttcg ggaccgaaat cggccgaggg gtcgtaatag acggtcaccg
    17341 gctggccggt gccgatcggg tcgacataat aggggttcgc gctcgttacc atgatcggta
    17401 cggtcccgtt gaggcggcgg cgcgctgcga accgtcggtc ggcatagagg gcgcgcgcga
    17461 acagcatcgt gtcgcccgca agccgctgct cgacgctggc atagccgcta gccgagcgtt
    17521 gcctgggcaa tatgtcgata ttgcgccgcg cgtcgccgcg gttgaaatcg ctggtcgcga
    17581 tgaggtcggc gcgcgtgagc cccgtgccgt cctgcccggg cggaatgagg aagcgctgcc
    17641 cattcgcggc gacaatcgtg ccgggaacat tgtagttcga gcgaaggtcg gggccgccga
    17701 acggacggag gtcctccgtc gcgaacgcgc gctgcgtcga ggggagggcg ccgcggcgct
    17761 gatattcgcc cgcgacgacg agactgccgc ccgcccattt ggttccgacg atctgcccgg
    17821 cgagaatttc gccatagtcg ccgtccgcgg tcccgacgcg cagccgcgtc tcggcgcctt
    17881 cgaagcgatt gcggaagcgg atgttgacca ctcccgcgat cgcgtccgac ccgtagatcg
    17941 ccgaggcgcc gtcggtgaga atctcgacgc gctcgatgat agccgatggg acgagcgaca
    18001 gatcggtgaa cgcgcccgag gccccgccga gcgccgggcg cgcgccgtcg aagagggtca
    18061 gcgtcgcgcc gggaccgagg ccgcgaagat tgatgccggt gccgaggccg atattgctga
    18121 tcgcattgcc gcgggcgctc atgccgatat tggcctcggt gggtccgccc gagaagttct
    18181 gcgggatcgt ctggatgaag tcagccagcg tcgcgcggcc gctgcgatcg agctcggcgc
    18241 gatcgatgac ggtgacgggc gagccgaccg gccccgttcc ccgaatgcgc gtgccggtca
    18301 cgatgatcgc cgcgtcggga ttcccggccg gcttcgcgtc cccgagtgcg ctgttctctt
    18361 gcgcgcgcag gacgagcgcc ccgtcgacca gctcggccat gaggccggtg cccgcaagca
    18421 tgcgggcgag ggcggcttcg ggcgagaggc ggccctttac accgctgctc cggcgatcct
    18481 cgaccaggct cgacgccgcg atgacgtcgc ggcccgaaac gcgcgaatat tcacggatcg
    18541 cgtcggcaag gccttgcgcc gcgatgtcgt aatcggtctg cccggtctgc gccgctgcgc
    18601 tcggtgcccc gatcgcgagg ccgcttcccg tcgccagcag cgccgccaga tatagtctct
    18661 tcatcaaacc ctcccttgca gccgcgtcct ggctgtctgg gtggctcgat gacgccgggg
    18721 gaatcgcccc cctttatttc cgcgggcgtt tgaggacggg gccggcggcg cccgcctcga
    18781 tgccgagatc gagcgcggcg gcgagcttgc gcgcgagcgc gtcgccgtcg tcgatttcga
    18841 agcgtccgct gacgcgcagc ccggcgaggg cgggatcggc gaggccgatc tttcgcgcgt
    18901 tcacgcggtt cgcgcgatcg acgatcgcgc cgagcgggag atcgtcggca tcgagcttgc
    18961 tcg
//
LOCUS       DATMSU010000115         5905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_163039,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000115 DATMSU010000000
VERSION     DATMSU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163039"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VN034_03115"
     CDS             <1..1348
                     /locus_tag="VN034_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HWV59617.1"
                     /translation="PGSGTIGSLTVTGEYTGAGGTLEIEAELGADGSLADRLIVGGAT
                     SGSTRVSVINRGGLGAQTVEGIKIIDIAGASNGNFTLDGDYLFDGEQAVIAGAYGYRL
                     YKNGIATPQDGDWYLRSALLDGPGEPEGPLYQPGVPLYENYGQVLQSLNGLPTLQERV
                     GNRQWARADDGASTGIWGRMESTRARPEARVSTADTDANIDSWKMQIGVDRTLSESGN
                     GTLVAGITGHYGESNAAVRSTFGDGSIEAKAYGAGATLTWYHQGGFYADAQAQFAWFD
                     GQLESSVLGTLVDGNDGTGKAFSLEVGKRAVVGHGLTVTPQIQMSYQDAGFDSFTDPS
                     GARVSSSRGDSLKSRWGISLDHQKSWQTDGTTRRSHLYGLVNLSYEWLDGTAADVSGT
                     AIRNANDRLWGEIALGGSLGLNDRVTLYTQASASSPFRDFGDDYSIKGTAGVRVAF"
     gene            complement(1420..2229)
                     /locus_tag="VN034_03120"
     CDS             complement(1420..2229)
                     /locus_tag="VN034_03120"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV59618.1"
                     /translation="MPLRATIQHWELSFHIYENILCQMTGPVRSVSHAFAIIRLLARS
                     NALSLSDIGRMIGISPSTCLNLLKTLTAEGVIEREAQKKQYRLTPPWRWADVLGDSRA
                     KRLVGRAQPAMMQFAQSHETAVGLWQIVSRDRMQLVTHAESDAGMRLTLADEQRQPLG
                     AGAAGRAIAAAQEIGEAELARRFAPVRWQADLSIATYVRQVEDAAAKGFALDRGYAHR
                     GVYTAAAALTDIAPGFCLTASFVAGSRTDSELETLGAALAELGNELVSLSA"
     gene            2265..3182
                     /locus_tag="VN034_03125"
     CDS             2265..3182
                     /locus_tag="VN034_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59619.1"
                     /translation="MIGCLDGKVVAITGAGRGIGRELAIHCAAQGAAVVVNDPGVAQA
                     GDGGDEAPAETTANDIVAAGGRAFANFGNVADPKEAASIVEDAVARFGRIDAVVNNAG
                     ILRDTIWHKMSYEDWKSVIEVNLTGVFNVSKAATPWFREQQSGSFIHFTSTSGLIGNI
                     GQANYSAAKLGVVALSQSIALDMARVGVRSNCIAPFAWSRMTSSIPATTPAEQERVKR
                     LQEMSADKIAPLVAYLASDLSKDVTNQIFSVRKNEILLFSKPRPLRSMAKLDGWTAQA
                     IADELIPAFRPDFARADEVSAHVFPYDPI"
     gene            3194..4363
                     /locus_tag="VN034_03130"
     CDS             3194..4363
                     /locus_tag="VN034_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV59620.1"
                     /translation="MSNPGMDADIFDQFIDQLERYVRARLIPAEQGIIESDEIPADIL
                     AEMRDMGLFGLTIPEEFGGAGMNVSQYIKTIHTISYAMPAFRSIISINIGMVAFALKN
                     GGTDAQKAEWLPRIAAGEIACFGLTEPGSGSDSAGLQATAVQTDNGWVLNGTKRYITN
                     SPFAKLGLIMARTSKENLPKNAHVSAFLVPLDSAGVTIGKPDKKMGQSGSHIADVIME
                     DVRVGGEALLGGELGKGFAYAMMSLDNGRMSVGAAATAYARRALDSAVRYATERKAFG
                     EPIANFQLIQQMLAQSEIDIYAAECMMEDVTRRADAGENVLRKAAAFKVFASEMCGRV
                     VDACVQVYGGAGYLAEYDAERFFRDARIYRIYEGTTQILQLQIAKHMLREFAAAN"
     gene            4567..5199
                     /gene="catB"
                     /locus_tag="VN034_03135"
     CDS             4567..5199
                     /gene="catB"
                     /locus_tag="VN034_03135"
                     /EC_number="2.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B chloramphenicol O-acetyltransferase"
                     /protein_id="HWV59621.1"
                     /translation="MTNFFESPFKGVTLDKQVTHPNIRVGRFSYYSGYYHGHSFDDCA
                     RFLLPDDDADKLIIGSFCSIGSGAAFIMAGNQGHRHDWISTFPFYWMPEVRAFEGARN
                     GFQPAGDTVVGNDVWIGSEAIVMPGVRIGDGAVIATRAVVTRDVAPYSIVGGNPAAPI
                     RTRFDAARIALLLEMRWWDWSDEQLHAAMPLLTDGKVEALHEYWLAGGSR"
     gene            5383..>5905
                     /locus_tag="VN034_03140"
     CDS             5383..>5905
                     /locus_tag="VN034_03140"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWV59622.1"
                     /translation="MIASLRLILLALFLAMAGIAPSSAQPMFSPNVEVSLVPMHRWAA
                     PGSTAIVAVRQDIKPGWHTYWRNPGDSGGATELTWELPAGFSAGLILWPLPERQPVKG
                     LMNYGYSHQVYLPVPIDVPRTAKSGSTVPLSVTVLSMVCSEDMCVPDERTVKFDLPVR
                     DGVPPLAGAEGAAI"
BASE COUNT         1068 a         1856 c         1922 g         1059 t
ORIGIN      
        1 gccgggcagc ggcaccatcg gctcgttgac cgtcaccggc gaatacaccg gcgccggcgg
       61 cacgctcgaa atcgaggccg aactcggcgc ggacggctcg ctcgccgacc gcctgatcgt
      121 gggcggcgcg acatcaggca gcacgcgcgt cagcgtgatc aatcgcggcg ggctcggcgc
      181 gcagaccgtc gaaggcatca agatcatcga catcgctggc gcgtcgaacg gaaacttcac
      241 gctcgacggc gattatctgt tcgatggcga gcaggcggtc atcgcgggtg cctatggcta
      301 ccggctctac aagaacggga tcgcgacgcc gcaggacggc gactggtatc tccgttccgc
      361 gttgctcgac gggccgggcg agccggaggg gccgctctac cagccggggg tgccgctcta
      421 tgaaaattac ggacaggtgc tgcagtcgct gaatggtctt ccgacgctgc aggaacgcgt
      481 gggcaaccgc caatgggccc gcgcggacga tggtgcgagc acgggtatct ggggccgcat
      541 ggaaagcacg cgcgcccggc ccgaggcccg cgtgtcgacg gcggacaccg acgccaatat
      601 cgatagctgg aaaatgcaga tcggtgtcga tcggacgctc agcgagagcg ggaatgggac
      661 gctcgtcgcg ggcatcaccg gccattatgg cgaatccaac gccgccgtcc ggtcgacctt
      721 cggcgacggc agcatcgagg cgaaggccta tggcgccggc gcgacactga cctggtatca
      781 tcaaggcggc ttttatgccg acgcgcaggc gcagttcgcc tggttcgacg gccagctcga
      841 atcttcggtg ctcggaacgc tggtcgacgg taacgacggc acgggcaaag cgttcagcct
      901 cgaagtcggc aaacgcgccg tcgtcggcca cggcctcacc gttacgccgc aaatccagat
      961 gagctatcag gatgccggtt tcgacagctt caccgatcca tccggggcga gggtttcgtc
     1021 gagcaggggc gacagcctca agtcgcgttg gggcatttcg ctcgatcatc aaaaatcgtg
     1081 gcagacggac ggaacgaccc ggcgcagcca tctctatggg ctggtcaatc tgagctacga
     1141 atggctcgac gggaccgccg ccgatgtgtc gggcaccgcg attcgcaacg ccaacgaccg
     1201 gctatggggc gagatcgccc tcgggggtag cctcggtctc aacgaccggg tgaccctcta
     1261 cacccaggca tcggccagct cgccgttccg cgatttcggc gacgattaca gcatcaaggg
     1321 gacggccggc gtgcgggtgg ccttttagct tccccatttc gaggtgattt ggggccatga
     1381 tcctgtcgcg aaatcgaggc gcgagctttt tttgaaaggc taggcggaaa gcgacaccag
     1441 ttcattgcca agttcggcga gcgccgcacc gagagtctca agctcgctat ccgtgcgcga
     1501 tccggcgacg aacgaagctg tgaggcagaa tccgggcgcg atgtcggtga gcgcggcggc
     1561 cgcggtgtac acgcctcgat gcgcatagcc gcgatcaagc gcaaatccct tcgcggcggc
     1621 gtcctcgact tgccgcacat aagtcgcgat cgacaggtcg gcctgccaac ggaccggcgc
     1681 gaagcgccgt gccaactccg cttcgccgat ctcttgcgcc gccgcgatcg cgcgccccgc
     1741 cgcacccgcg cccagcggct gccgctgttc gtccgcaagg gtcagccgca ttccggcatc
     1801 gctttccgca tgcgtaacga gctgcattcg atcgcgcgaa acgatctgcc ataacccgac
     1861 tgccgtttca tgcgactgcg caaactgcat catggcgggt tgtgcccgac cgacaagccg
     1921 cttcgcccgg ctgtcaccga ggacatccgc ccaccgccat gggggcgtca ggcgatattg
     1981 ttttttttgg gcctcgcgtt cgatcacgcc ttcggccgtg agggtcttta gcaggttgag
     2041 gcaggttgat gggctgattc cgatcatccg gccaatgtcg gacagcgaca gcgcgttcga
     2101 ccgcgcgagc aggcggatga tcgcgaaggc gtgcgaaacg gagcgtaccg ggccggtcat
     2161 ttggcaaaga atattttcat atatatgaaa tgacaattcc caatgttgaa ttgttgcgcg
     2221 caaaggcata tcgcgaactt ggcggtaatc gaatggagaa caccatgatc gggtgcctcg
     2281 acggaaaggt cgtcgctata accggcgccg ggcgcgggat tggccgcgaa ctggcgattc
     2341 attgtgcggc gcagggagcg gcggtggtcg tcaacgatcc cggcgtggcg caagccggcg
     2401 acggtggcga cgaggcaccg gccgagacca ccgcgaacga catcgtcgcg gcgggcggcc
     2461 gggctttcgc caatttcggc aacgtcgccg atcccaaaga ggccgcgagc atcgtcgagg
     2521 acgcggtcgc gcgcttcggc cgcatcgacg cggtagtcaa taacgccggc atccttcgcg
     2581 acaccatctg gcacaagatg agttacgagg actggaagag cgtcatcgag gtcaatctga
     2641 ccggcgtctt caacgtgtcg aaggccgcga cgccctggtt tcgcgagcag cagtcgggaa
     2701 gcttcatcca cttcacctcg acgagcgggc tgatcggcaa catcggacag gccaattact
     2761 cggcggccaa gctcggcgtc gttgcgctgt cgcagtcgat cgcgctcgac atggcgcgcg
     2821 tcggcgtccg ctcgaactgc atcgcgccct ttgcatggag ccgcatgacc tcgtcgatcc
     2881 cggcgacgac gcccgccgag caggagcgcg tcaagcgcct gcaggaaatg tcggccgaca
     2941 agatcgcgcc gctcgtcgcc tacctcgcat ccgatctgtc gaaggatgtg acgaaccaga
     3001 tcttcagcgt ccgcaagaac gagattctgc tcttctcgaa gccgcgcccg ctccgttcga
     3061 tggcaaagct cgatggctgg acggcgcagg cgattgccga tgaactgatc ccggcgttcc
     3121 gccccgattt cgcgcgcgcc gacgaagtgt cggcgcatgt tttcccctac gaccccattt
     3181 gaggatcacc gcgatgagca atcccggcat ggacgccgac attttcgatc agttcatcga
     3241 ccagctcgaa cgctatgtgc gcgcgcggtt gattcccgcc gagcagggca tcatcgagag
     3301 tgatgaaatt cctgccgaca tccttgccga aatgcgcgac atgggccttt tcggcctgac
     3361 catccccgaa gaatttggcg gcgcggggat gaatgtcagc cagtatatca agacgatcca
     3421 cacgatcagc tatgcgatgc cggcgttccg ctcgatcatt tcgatcaaca tcggcatggt
     3481 cgccttcgcg ctcaaaaatg gcggaaccga cgcgcagaag gccgaatggc tgccgcgcat
     3541 cgcggcgggg gagatcgcct gtttcggcct gaccgagccg ggttccgggt cggattcggc
     3601 ggggctgcag gcgaccgcgg tgcagaccga caatggctgg gtgctcaacg gcacgaagcg
     3661 ctacatcacc aactcgccct tcgcgaagct cgggctgatc atggcgcgga cgagcaagga
     3721 gaatctgccg aagaatgccc atgtctcggc ctttctggtg ccgctcgaca gcgccggcgt
     3781 gaccatcggc aaaccggaca agaagatggg ccagtcgggc agtcatatcg ccgacgtcat
     3841 catggaggat gtccgcgtcg gcggagaggc gctgctcggc ggtgagcttg gcaagggctt
     3901 cgcttacgcg atgatgagcc tcgacaatgg ccgcatgtcg gtcggcgctg cggctaccgc
     3961 ttacgcccgc cgcgcgctcg acagcgcggt gcgctatgcg accgagcgca aggcgtttgg
     4021 cgagccgatc gcgaacttcc agctcatcca gcagatgctc gcgcagagcg agatcgacat
     4081 ctacgccgcc gaatgcatga tggaggatgt cacgcgccgc gccgatgcgg gagagaatgt
     4141 cttgcgtaag gccgcggcgt tcaaggtgtt tgcgagcgaa atgtgcggcc gcgtcgtcga
     4201 tgcctgcgtc caggtctatg gcggcgccgg ctatctcgcc gaatatgacg ccgagcgctt
     4261 tttccgcgat gcgcgcatct atcgcatcta tgaaggcacg acgcagatcc tgcagcttca
     4321 gatcgccaag catatgctgc gcgagttcgc tgccgcaaac tgatccgcgc cccttcgaat
     4381 tgcgttgctc aaagccgggt gctaggcatc gcagcgatga aatcggcacc atgtaccacc
     4441 tccatcgggt tcccggtcgg ctgacgcgag gaacatgagc aggcgctcca aacggagcgc
     4501 ctgaatggag gcgattgcct tcgcggcgcg gcacctgggt gcccgctttt cctcaggtac
     4561 atggaaatga cgaatttttt cgaaagcccg ttcaagggcg tcacgctcga caagcaggtg
     4621 acccacccca atattcgtgt cgggcgtttc agctattatt cgggctatta tcacgggcac
     4681 agtttcgacg attgcgcgcg gttcctcctt cccgacgacg atgccgacaa gctgatcatc
     4741 ggcagtttct gctcgatcgg atcgggcgcc gccttcatca tggcgggcaa tcagggccat
     4801 cggcacgact ggatcagcac ctttcccttt tactggatgc cggaggttcg cgcattcgag
     4861 ggcgcccgca acggctttca gcccgcgggc gacacggtgg tcggcaatga tgtgtggatc
     4921 gggtccgagg cgatcgtcat gcccggggtc cggatcggcg atggcgccgt gatcgcgacg
     4981 cgcgcggtcg tgacccgcga cgtcgcgccc tattcgatcg tcggcggcaa ccccgccgcg
     5041 ccgatccgga cgcggttcga cgcggcgcgc atcgccttgc tgctcgagat gcggtggtgg
     5101 gactggagcg acgagcaatt gcacgcggcc atgccgctcc tcaccgacgg aaaggtcgag
     5161 gcgctgcacg aatattggct ggccggcggc tcgcgctgag cccgcggggg tgggagggca
     5221 gagccttctc accccatgtc ccgatccgcc cggccagcct cgcgtcgcgg tcagcttcga
     5281 tcaggatatt tgattctgcc ataatgttgc gacaaagtcg tgatgctcct gtcccgcggc
     5341 aaaccggggc gtcgcgttgg aaagctgtcg acaggtccat ccatgatcgc ttcgctccgc
     5401 ctgatccttc ttgccctttt cctcgccatg gcgggcatcg cgccgagttc ggcgcagccg
     5461 atgttttcgc cgaacgtcga agtctcgctg gtgccgatgc atcggtgggc ggcccccgga
     5521 tcgacggcga tcgtcgcggt ccgtcaggat atcaagcccg gatggcatac ctattggcgc
     5581 aatccggggg attcgggcgg cgcgaccgaa ttgacgtggg agcttccggc cggattctcg
     5641 gcggggctga tcctgtggcc gcttcccgaa cgccagccgg tcaaggggct gatgaactac
     5701 ggctattcgc accaggtcta tctgccggtg ccgatcgacg tgccgcggac ggcaaagtcc
     5761 ggatcgacgg tgccgctgag cgtcacggtc ctctcgatgg tctgcagcga ggatatgtgc
     5821 gttcccgacg agcggacggt caaattcgat cttcccgtcc gcgatggcgt gccgccgctc
     5881 gccggtgccg aaggcgccgc gatcg
//
LOCUS       DATMSU010000116         2590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_59125, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000116 DATMSU010000000
VERSION     DATMSU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59125"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2590
                     /locus_tag="VN034_03145"
     CDS             <1..>2590
                     /locus_tag="VN034_03145"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HWV59623.1"
                     /translation="DDSIRAMMLIRTYRVRGHLAATLDPLDLSQRELPADLTPEYHGF
                     AGADLDRPVWLGGTLGLERATVREIVAILRANYCGHVGLEYMHISDVEERRFLQDRME
                     GADKSVEFTQEGKRAILNKVIEAEEWEKFLARKYVGTKRFGLDGGESMIPAMESIIKY
                     GGQYGVKEIVYGMAHRGRLNMLANVMAKPYQVIFHEFAGGSANPDDIGGSGDVKYHLG
                     TSTDREFDGVSVHMSLVPNPSHLEAVDPVVLGKVRAQQVVRDDLAEHTQVLPVLIHGD
                     AAFAGQGIVWECLGFSGIRGYNTGGCIHFIVNNQIGFTTSPQFARSSPYPSDVAKGVQ
                     APILHVNGDDPEAVTFACKLAIDFRQQFKRDVVIDMWCYRRFGHNEGDEPSFTQPLMY
                     ERIRKHPPVSQLCAAKLEDEGVIDAGWADARRAEFTTRLEGDFEAAKTYKPNKADWFA
                     GRWSGLHAPADPENARRNVATGVSEKLFDSIGRTLTTIPADVEVHKTLRRVIDARGAM
                     FADKKDGEVFDWATAESLAFGTLLSEGYQVRLSGQDSGRGTFSQRHAVWVDQKSEQKY
                     VPLTTVPHGRFEVLDSPLSEYGVLGFEYGYAMADPKSLVLWEAQFGDFANGAQIMIDQ
                     FIAAGEAKWLRANGLVLLLPHGYEGQGPEHSSARLERFLQLCAGDNIQVCNISTPSNY
                     FHVLRRQMLRSFRKPLIIMTPKSLLRHKLAVSQRSDFIGDAHFRRIMSDRTPPADKDI
                     KRVVLCSGKVGYDLMEARDAAGLTDTTVIRIEQLYPFPGEALAVRLKRMPKLEEVVWA
                     QEEPRNNGAWFFAGELIEESLIEAGHKGMRPRYAGRAAAASPATGLMSRHQTEQSALV
                     ADALGLS"
BASE COUNT          464 a          855 c          836 g          435 t
ORIGIN      
        1 gacgattcga tccgcgcgat gatgctgatc cgcacctatc gcgtgcgcgg ccatctggcc
       61 gctacgctcg atccgctcga cctcagccag cgcgagctgc ccgcggattt gacccccgaa
      121 tatcacggct tcgccggcgc cgatctcgac cgtccggtct ggctcggcgg cacgctcggc
      181 ctcgagcgcg cgacggtgcg cgagatcgtc gcgattctgc gcgccaatta ttgcggtcat
      241 gtcggcctcg aatatatgca catctccgac gtcgaggagc ggcggttcct gcaggaccgg
      301 atggagggcg ccgacaagag cgtcgaattc acccaggaag gcaagcgcgc gattctcaac
      361 aaggtgatcg aggccgagga atgggagaaa ttcctcgcgc gcaaatatgt cggcaccaag
      421 cgtttcgggc tcgacggcgg cgaatcgatg atccccgcga tggaatcgat catcaaatat
      481 ggcggccaat atggcgtaaa ggaaatcgtc tatggcatgg cgcaccgcgg gcggctcaac
      541 atgctcgcga acgtcatggc caagccctat caggtgatct tccacgaatt cgccggcggc
      601 tcggcgaacc ccgacgacat cggcggctcg ggcgacgtca aatatcacct cggcacgtcg
      661 accgaccgcg agttcgacgg tgtttcggtg catatgtcgc tggtgcccaa cccgtcgcac
      721 ctcgaggcgg tcgatccggt cgtgctcggc aaggtgcgcg cgcagcaggt ggtgcgcgac
      781 gatctcgccg aacatacgca ggtgctgccg gtgctgatcc acggtgacgc ggcgttcgcg
      841 gggcagggga tcgtctggga atgcctcggt ttttcgggca tccgcggcta caacaccggc
      901 ggctgcatcc acttcatcgt caacaaccag atcggtttca cgaccagccc gcagttcgcg
      961 cgctcgtcgc cctatccgtc ggacgtcgcc aagggcgtcc aggcgccgat cctgcacgtc
     1021 aacggcgacg atcccgaagc ggtgaccttc gcgtgcaagc tcgcgatcga tttccgccag
     1081 cagttcaagc gcgatgtcgt gatcgacatg tggtgctatc gccgcttcgg ccataacgaa
     1141 ggcgacgagc cgtcgttcac ccagcccttg atgtacgaac gcatccgcaa gcatccgccg
     1201 gtgtcgcagc tttgcgcagc gaagctggag gacgagggcg tgatcgacgc cggctgggcc
     1261 gacgcgcgcc gcgccgaatt cacgacgcgg ctcgaaggcg atttcgaggc ggcgaagacc
     1321 tataagccca acaaggccga ctggttcgcg ggccgctggt cggggctgca cgcccccgcc
     1381 gaccccgaaa acgcacgccg caacgtcgcg accggcgtgt cggaaaagct gttcgattcg
     1441 atcggccgca cgctgacgac gatccccgcc gacgtcgagg ttcacaagac gctgcgccgc
     1501 gtcatcgacg cgcgcggcgc gatgttcgcc gacaagaagg atggcgaagt gttcgactgg
     1561 gcgaccgccg aaagcctcgc tttcgggacc ttgctcagcg aaggctatca ggtgcgcctg
     1621 tcgggacagg attcggggcg cggcacgttc agccagcgcc acgcggtctg ggtcgaccag
     1681 aagagcgagc agaaatatgt cccgctgacg accgtgccgc acggccggtt cgaagtgctc
     1741 gacagcccgc tctccgaata tggcgtgctc ggcttcgaat atggctatgc gatggccgac
     1801 ccgaagagcc tcgttctctg ggaagcgcag ttcggcgact tcgccaacgg cgcgcagatc
     1861 atgatcgacc agttcatcgc cgcgggcgag gccaagtggc tgcgcgccaa cggcctcgtg
     1921 ctgctgctgc cgcacggtta tgaagggcag gggcccgagc atagctcggc gcgccttgaa
     1981 cgcttcctgc agctttgcgc gggcgacaat atccaggtgt gcaatatttc gaccccgtcg
     2041 aactatttcc acgtcctgcg ccgccagatg ctgcgctcgt tccgcaagcc gctgatcatc
     2101 atgacgccca agtcgctgct gcgccacaag ctcgcggtgt cgcagcggtc ggacttcatc
     2161 ggcgacgcgc atttccgccg catcatgtcc gaccgcacgc cgcccgccga caaggacatc
     2221 aagcgcgtcg tcctctgttc gggcaaggtc ggttacgacc tgatggaggc gcgcgacgcc
     2281 gcgggtctga ccgacacgac ggtgatccgc atcgagcagc tctatccctt cccgggcgag
     2341 gcgctcgcgg ttcgcctgaa gcggatgccg aagctcgaag aggtcgtctg ggcgcaggaa
     2401 gaaccgcgca acaatggcgc ctggttcttc gcgggcgaac tgatcgagga atcgctcatc
     2461 gaggcgggcc acaagggcat gcgcccgcgt tatgcgggcc gcgccgccgc cgcgtcgccc
     2521 gcgaccggtc tgatgagccg ccaccagacc gaacagtcgg cgctcgtcgc cgacgcgctc
     2581 ggcctttcgg
//
LOCUS       DATMSU010000117         1734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_182455,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000117 DATMSU010000000
VERSION     DATMSU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182455"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VN034_03150"
     CDS             complement(<1..808)
                     /locus_tag="VN034_03150"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HWV59624.1"
                     /translation="MEKLTVELGARSYPILIGDGLIREIGGHVAPLLKRPRTMIVTDA
                     HVADHYLIPLGTALAMENISFSSFVLDPGESTKSWAGLARLTEWLIGEGVERGDHVIA
                     LGGGVIGDLVGFACSIVKRGCHFIQVPTTLLAQVDSSVGGKTAINVPAGKNLIGAFHQ
                     PAMVVIDPTTLETLPRRELGAGYAEVVKYGLIDDADFFAWCEANGAALLAGDSAARHK
                     AIAHSVAAKARIVAADERETQDIRALLNLGHSFGHALEAETGYSDRLLHGE"
     gene            complement(796..1374)
                     /locus_tag="VN034_03155"
     CDS             complement(796..1374)
                     /locus_tag="VN034_03155"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWV59625.1"
                     /translation="MSRNPKSPAAAIPASIRDRSIVLVGLMGSGKSTIGRRLAQRLGM
                     RFADADDEIERAAGMTISDIFSRFGEAHFRDGERRVIARLLAGKPMVLATGGGAFVNE
                     ETRALILEDSLCIWLDADIPTLVERVGRRSHRPLLKGRDPGEVLRELAAVRNPIYAEA
                     HLRVSSASTPHDHTVRAILEALSQWKASQWKS"
BASE COUNT          286 a          576 c          584 g          288 t
ORIGIN      
        1 cctcgccgtg cagcagccgg tccgaatagc ccgtctcggc ttcgagcgcg tggccgaagc
       61 tgtggccgag gttgagcagc gcgcggatat cctgcgtctc gcgctcgtcg gcggcgacga
      121 tgcgtgcctt ggccgcgacg ctgtgcgcga tcgccttgtg ccgtgccgcg ctgtcgcccg
      181 cgagcaaagc cgcgccgttc gcttcgcacc aggcgaaaaa gtccgcgtcg tcgatcagcc
      241 catatttgac gacctcggca tagccggcgc cgagttcgcg gcgcggcagc gtttcgagcg
      301 tcgtgggatc gatcacgacc atcgcgggct gatgaaaggc gccgatcaga ttcttgcccg
      361 cgggcacgtt gatcgccgtc ttgccgccga cgctgctgtc gacctgcgcc aacaaggtgg
      421 tcgggacctg gatgaaatgg cagccgcgct tgacgatgct gcacgcgaaa ccgacgagat
      481 cgccgatcac gccgccgccc agcgcgatga catggtcgcc gcgctcgacg ccttcgccga
      541 tcagccattc ggtcaggcgc gcgagccccg cccagctttt cgtgctttcg ccgggatcga
      601 gcacgaagga ggaaaaggag atattctcca tcgccagcgc ggtgccgagc ggaatcagat
      661 aatggtccgc gacatgcgcg tcggtgacga tcatcgttcg cggacgcttc aagagcggcg
      721 cgacatgccc gccgatctcg cggatcagcc cgtcgccgat caggatcgga tagctgcgag
      781 cgcccagttc gacggttagc ttttccattg cgacgctttc cattgcgaca gggcctccaa
      841 tatcgcgcgc accgtatggt cgtgcggcgt gcttgccgaa ctgacgcgca gatgcgcctc
      901 ggcgtagatg ggattgcgga ccgccgcgag ttcgcgcagc acttcgcccg gatcgcggcc
      961 cttcaacagc gggcggtggc tgcggcgccc gacacgttcg accagcgtgg gaatgtcggc
     1021 gtcgagccag atgcagaggc tgtcctcgag gatcagcgcg cgcgtttcct cgttgacgaa
     1081 ggcgccgccg ccggtcgcga gcaccatcgg ctttccggcg agcaggcgcg cgatcacgcg
     1141 gcgctcgccg tcgcggaaat gcgcctcgcc aaagcgcgaa aaaatgtccg agatcgtcat
     1201 gcccgcagcc cgctcgattt cgtcgtcggc gtcggcgaag cgcatgccca gccgctgcgc
     1261 gaggcggcgg ccgatcgtcg actttcccga gcccatcagc ccgacgagca cgatcgaccg
     1321 gtcgcggatc gacgcgggga tagcagcagc cgggcttttc gggtttcgcg acatggtgcg
     1381 cgcggctata ccgcccgttc ggcgcggaac aatggttttg cgcgatacgg gacgaacggc
     1441 gggcattgga cattgcgcgc cgggaccgga tcgggaaaag gcgggctgcg gcgtcaatga
     1501 ccgatcgggc cggaatgatt gaggtgaatg tgatcgacca gattgccgaa ggcgcggcgc
     1561 gatgaagaaa aacacgctgc cgttgacgtt gggcctgtcg gccaccgcgc tcgccgccat
     1621 attggtgctg cccgcgatcg cgcaggaatc gttgttgccc gaagggttcg gcacccccgc
     1681 cgacacgccc gcgccgaaat cttcgccgac gccgtccccg tcgcccaccg cgac
//
LOCUS       DATMSU010000118         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_77761, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000118 DATMSU010000000
VERSION     DATMSU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77761"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="VN034_03160"
     CDS             complement(<1..365)
                     /locus_tag="VN034_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HWV59626.1"
                     /translation="MPKHRAPYDRSKRPLGAAKLDELALAYVARFATSRAKLTRYLSR
                     KVRESEWIDDSDAMAACAAIADRMEHLRYLDDRQYAAMRAGAMTRRGLGARRVKAQLY
                     ADGIAEQDSGAAIEAAEGAA"
     gene            559..2310
                     /locus_tag="VN034_03165"
     CDS             559..2310
                     /locus_tag="VN034_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HWV59627.1"
                     /translation="MTDTDMISADALVPTPTECAQPRRFSDFATVGEALDYAAAGSRG
                     LNFHDPRGKLVRPYPYSELKADALATAYRLIAAGVQPNDRIALIAETGVEFAALFFGT
                     IYAGAWPVPLPLPTSFGGRDSYVGQLVVQLTSCDPKMLFFPPEIAAMAIEAAEQRKVQ
                     PIDWSEFEKRPAPVADLPEQKTDETCYLQYSSGSTRFPHGVAVTHGALLNNLSAHSHG
                     MELQDSDRCISWLPWYHDMGLVGCLLSPVANQVSVDYLKTEDFARRPLAWLDLISRNQ
                     GTTLSYSPTFGYDICARRISSQSHVADRFDLSRWRVAGNGADMIRPDVMQSFVDAFAD
                     AGFKASAFLPSYGLAEATLAVSIMPPGEGIVVELVEETELSGAANDSGRPTRYRAIVN
                     CGKAVKDMVIEVRDEAGNPLADQTVGKVWCTGPSLMTGYFRDSESTAACMADGWLDTG
                     DMGYMSDGYIYIVGRAKDMIIINGKNHWPQDIEWAVEQLPGFKSGDIAAFAITTPGGE
                     ETPAVLVQCRTSDEAERLALRETIRDRVRAITGMNCLIELIPPRTLPRTSSGKLSRSK
                     ARAQYLAGEIQPFAMAA"
     gene            complement(2522..>2740)
                     /locus_tag="VN034_03170"
     CDS             complement(2522..>2740)
                     /locus_tag="VN034_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWV59628.1"
                     /translation="ARDALKLAPGAQAFNMAFPIHASKVGGWAWRIVLTISGLSLTLL
                     GSLAVWTFWFRRPKPAKRPVKKAALASA"
BASE COUNT          467 a          951 c          853 g          469 t
ORIGIN      
        1 gccgcgcctt ccgcggcctc gatcgccgcg ccgctgtcct gctcggcgat gccatcggca
       61 taaagctgcg ccttgacccg ccgcgccccc aatccgcgcc gcgtcatcgc cccggcgcgc
      121 atcgcggcat attgccggtc gtcgagatag cgcaaatgct ccatccggtc ggcgatcgcc
      181 gcgcacgccg ccatggcgtc actatcgtct atccattcgg attcgcggac ttttctggac
      241 agatagcgcg tcagcttggc gcggctcgtc gcgaatcgcg cgacataggc gagcgccagt
      301 tcgtcaagct ttgccgcgcc gagaggcctt ttcgatcggt cgtatggagc acgatgcttg
      361 ggcatatgcc atgtttgtgc cacagtcggg cccgattgag aacgatcaac accgccatat
      421 cgggcgccaa agcccggaaa tgggcgattt gttcaaacaa catgttcaaa caacacaggg
      481 ttcgcgcacc gacacatggc acagaaagat gacatgcttg ttgccgcgcg gcccgtatcg
      541 cgggatgtcg cagcccctat gaccgatacc gacatgattt cggccgatgc gctcgtgccg
      601 acgccgaccg aatgcgcgca gccgcgccgt ttttccgact tcgccaccgt gggcgaggcg
      661 ctcgactatg ccgccgcggg ttcgcgcggg ctcaatttcc acgatccgcg cgggaaactc
      721 gtgcgcccct acccctatag cgagctcaag gccgacgcgc tggccaccgc atatcgtttg
      781 atcgccgccg gggtgcagcc gaacgaccgc atcgcgctga tcgccgaaac cggcgtcgaa
      841 ttcgccgcgc tcttcttcgg caccatctat gccggcgcct ggccggtgcc gctgccgctg
      901 ccgacgagct tcggcgggcg cgattcctat gtcggccagc tcgtggtcca gcttacgagc
      961 tgcgatccca aaatgctgtt tttcccgccc gaaatcgccg cgatggcgat cgaggcggcc
     1021 gaacagcgga aggtgcagcc gatcgactgg agcgagttcg aaaaacgccc cgctcccgtc
     1081 gccgacctgc ccgagcaaaa gaccgacgag acctgctatc tccagtatag cagcggctcg
     1141 acgcgtttcc cgcacggcgt cgcggtgacc cacggcgcgc tgctcaacaa tctgtcggcg
     1201 cattcgcacg gcatggagtt gcaggacagc gaccgctgca tttcgtggct gccctggtat
     1261 cacgacatgg gcctcgtcgg ctgcctgctc tcgcccgtcg ccaaccaggt gtcggtcgat
     1321 tatctgaaga ccgaggattt cgctcgccgc ccgctcgcct ggctcgacct gatcagccgc
     1381 aatcagggca cgacgctcag ctattcgccg accttcggct atgacatttg cgcgcgccgc
     1441 atctcgagcc agagccatgt cgccgaccgt ttcgacctgt cgcgctggcg cgtcgcgggc
     1501 aatggcgccg acatgatccg ccccgacgtg atgcaaagct tcgtcgacgc ctttgccgac
     1561 gccgggttca aggcgagcgc cttcctgccg agctatggcc ttgccgaagc gacgctcgcg
     1621 gtgagcatca tgccgccggg cgaggggatc gtcgtcgaac tggtcgagga aaccgagttg
     1681 tcgggcgccg cgaacgacag cggccgtccg acccgctacc gcgcgatcgt caactgcggc
     1741 aaggcggtca aggatatggt gatcgaggtc cgcgacgaag cgggcaatcc gctcgccgac
     1801 cagaccgtcg gcaaggtgtg gtgcaccggc ccgtcgctga tgaccggcta tttccgcgac
     1861 tccgaatcta ccgccgcctg catggcggac ggctggctcg acaccggcga tatgggttat
     1921 atgtcggacg gttacatcta tatcgtcggc cgcgccaagg acatgatcat catcaacggc
     1981 aagaatcact ggccgcagga tatcgagtgg gcggtcgagc agctgccggg cttcaagtcg
     2041 ggcgacatcg cggcctttgc gatcacgacc ccgggcggcg aggaaacccc cgcggtgctc
     2101 gtccagtgcc gcaccagcga cgaggccgaa cggcttgcgc tgcgcgaaac gattcgcgac
     2161 cgcgtccgcg cgatcaccgg catgaactgc ctgatcgagc tgatcccgcc gcgcaccctg
     2221 ccgcgcacca gctcgggcaa attgagccgc tcgaaggcgc gcgcgcaata tctcgccggc
     2281 gaaatccagc ccttcgcgat ggcggcctag ggccggacgc gcaggccttt ggccggtggc
     2341 tgtcgttctt ctcccctccc gcttgcggga ggggttgggg gtgggcggca acggtgcgat
     2401 tgcccacccc gctgcaacta gcgaacaagt tcgcaagttt cgctgcccct cccgccggcg
     2461 ggaggggaaa ccagctcaac tcctaaacgg ccgctcccca cctctgaacg gacgccggcg
     2521 cttatgccga tgccagcgcc gctttcttca ccgggcgctt cgcgggtttc ggccgcctga
     2581 accagaaggt ccacaccgca aggctgccga gcagcgtcag cgacaggccc gagatcgtca
     2641 gcacgatacg ccacgcccag ccgccgacct tcgacgcgtg gatcgggaag gccatgttga
     2701 acgcctgcgc gccgggcgcg agcttcagcg cgtcgcgcgc
//
LOCUS       DATMSU010000119         8877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_2358, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000119 DATMSU010000000
VERSION     DATMSU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8877
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..917)
                     /locus_tag="VN034_03175"
     CDS             complement(105..917)
                     /locus_tag="VN034_03175"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HWV59629.1"
                     /translation="MGRLHLHLISDSTGETLENIAKAAIAQFDDVEVVRHFWPMVRSE
                     SHLDRIMAEVQASPGMILFTLVNGELRVSLERRATALNLPTVAALDAVTDALSRMLGQ
                     EAKARPGRQHALDAAYFARVEAIQFTVAHDDGIGWENWEQADIVLAGVSRTSKTPTSI
                     YLANRGFKTANIPIVPESPPPSALYTLKRPMIVGLTTGLDRLVQVRRNRLLSLNQAPE
                     TSYVDDERVKAELAYARRMFADNGWPVIDVTRRSIEETAAAVIKLVEDRSAA"
     gene            1267..2295
                     /gene="hemE"
                     /locus_tag="VN034_03180"
     CDS             1267..2295
                     /gene="hemE"
                     /locus_tag="VN034_03180"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWV59630.1"
                     /translation="MKASPKKLLATLRGARQERPPLWLMRQAGRYLPEYRALRETKGG
                     FLELCYDPEAAAEVTLQPIRRFGFDGSILFSDILVIPHALGQHLWFEAGEGPRLAPPL
                     VDSALASLEAAHQRLDPVYATVTRVAASLPSETTFLGFAGSPWTVATYMVAGQGSKDQ
                     ATARRLAFGDPAAFQAIIDAIVELTVTYLSGQIENGVDAVQLFDSWAGSLSPAQYEQW
                     VIAPNAEIVRRLRALHPDTPIIGFPKGAGGKLRAYADETGVDAIGLDETVDPLWADAI
                     LPAHLPVQGNLDPLALVAGGEALDTAIDRILAAFPSRPHIFNLGHGIVPDTPIAHVEH
                     LIRRVRGG"
     gene            2299..2745
                     /locus_tag="VN034_03185"
     CDS             2299..2745
                     /locus_tag="VN034_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWV59631.1"
                     /translation="MELAGFLGATMLWIKAAHIIFVIFLMAGLFMMPRFFVYHHQCPV
                     GSDEDKKWIEREDRLRRIILNPALIIVWIFGLMLAFNGDYWHEGWFIAKFLLVIALSG
                     YHGWMIGYAKKLARGERPLSEKRLRMLNEVPGVAAAIIVILVVVRP"
     gene            2941..4197
                     /gene="rho"
                     /locus_tag="VN034_03190"
     CDS             2941..4197
                     /gene="rho"
                     /locus_tag="VN034_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWV59632.1"
                     /translation="MHLKELKTKAPAQLVEMAEELGVEGASTLRKQDLMFSILKELAE
                     EGEEIIGSGTIEVLPDSFGFLRSSEANYLAGPDDIYVSPNQVRKYGLRTGDTVEGEIR
                     APKDGERYFALTKLIKVNFDDPEAVRHRVNFDNLTPLYPNQKLSLDTVDPTVKDKSAR
                     VIDLVSPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVYLIVLLVDERPEEVTD
                     MQRSVKGEVVSSTFDEPATRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSRMDEVIFEE
                     FKGTGNSEIVLDRKVADKRIFPSLDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGT
                     IDAMEFLLDKIKDSKTNEDFFDSMNQ"
     gene            4207..4500
                     /locus_tag="VN034_03195"
     CDS             4207..4500
                     /locus_tag="VN034_03195"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWV59633.1"
                     /translation="MAVIEHALLHVRAGSEDAFEAAVAEARPLIEASPGFISLEIRRP
                     TGTGQPYLLLVEWRSVADHRDGFRQSDRYQQWRALLHHFYDPMPEVSYFGEPL"
     gene            4500..5231
                     /locus_tag="VN034_03200"
     CDS             4500..5231
                     /locus_tag="VN034_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWV59634.1"
                     /translation="MLEILTQAGAAGAHAAGFGGPADMWAAIVNDFSNITEPAAFAAF
                     LQVLMIDLVLAGDNAIVVGALAAGLPAEQRKKVILIGVIAALVLRIAFALVVTQLMQI
                     VGLIFVGGLLLIWVAWKMWRELRHTGQSHGSPEIAGDEHSGLKPAKSFAGAAWAVAVA
                     DVSMSLDNVLAVAGAAREHPGILIVGLIFAVALMGLAANIIAKYIERYRWIAYLGLAV
                     IVYVAVKMIYEGWVDPGVGVGTLFG"
     gene            5336..5722
                     /locus_tag="VN034_03205"
     CDS             5336..5722
                     /locus_tag="VN034_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HWV59635.1"
                     /translation="MTRVAVGCSDYPALQARIANYAQKGEIRFTTRFRPKRADELIGG
                     KLHFIVKHTLVGRVEILRFEDRSDGRVDIVCVAALERIHPQPKRAHQGWRYLAGSDAP
                     KGISEDDGLADLPPELYRELAELSLI"
     gene            complement(5719..7296)
                     /locus_tag="VN034_03210"
     CDS             complement(5719..7296)
                     /locus_tag="VN034_03210"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HWV59636.1"
                     /translation="MHRWIAFPVLLAAIALATWWSPARWTLILWVPLLLVAIYDAIQR
                     HHSLRRNFPLIARIRWLFEDLRPFLYAYIVEGPLDGRPFARAERDIIYARAKGELDSH
                     PFGTELDVYSDEYEWMSHSIAPVMGHDRTQRPVVGTSQCSKPYRPALLNISAMSFGSL
                     GANAIEALNLGARLGNFYHDTGEGGFSPYHRTHGGDIVWELGSGYFGCRDAEGRFDPE
                     RFRDRAAEDQVRMIEIKLSQGAKPGHGGVLPGAKVSAEIAATRGVPEGQDCISPAAHS
                     AFDTPIEMLEWAARLRELSGGKPVGIKLCVGQPHEIYAVMKAMIATGIRLDYIVVDGA
                     EGGTGAAPVEFSNRVGMPLREGLIFVRNALVGCGLKEEIRLAASGKVHSGAGLAVNCA
                     LGADWSNAARAFMFSLGCVQSLKCHTDMCPTGVATQDPGRQSGLVVEDKAERVRRFQA
                     ATVSALWDISCAMGLDDPWAIRPHHLHERLNSARSDSIDRIYKFYDRGVLLEDPGSVA
                     SARYWAMARAESFRAAF"
     gene            complement(7311..7709)
                     /locus_tag="VN034_03215"
     CDS             complement(7311..7709)
                     /locus_tag="VN034_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59637.1"
                     /translation="MTDHAGLAAWHAYMAGGGDPQALRDLLAEDAVFHSPVVHTPQEG
                     ADKVFAYLHAASHVLGGDDFRYLREIADGDQAMLEFQSNLDEIQINGVDIITWNEDGK
                     IKDFKVMVRPLKAINKVWEKMAAMLAAQAG"
     gene            complement(7706..8404)
                     /locus_tag="VN034_03220"
     CDS             complement(7706..8404)
                     /locus_tag="VN034_03220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV59638.1"
                     /translation="MAATNTNIPTLDGAGTLPAYVARPDADSARAIIVIPEIFGVNEG
                     IRKKCDDWAAEGYLAIAPDIFWRFAPGVELSPDIEAEFQQALGYFGQYDADDGVKDIE
                     AAIHWLRGEGAERIGCVGFCLGGRLAYMAATRTDIDASVGYYGVMIDQMLNEAHAIAN
                     PLMLHIPTEDHFVDHAAQKKIHEALDPHPKVTLHDYPGLDHGFAATMGNRRDEEGAQL
                     ADGRSRAFFAEKLA"
     gene            8514..8807
                     /locus_tag="VN034_03225"
     CDS             8514..8807
                     /locus_tag="VN034_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HWV59639.1"
                     /translation="MKFNIEIDCTPEEARRLFGLPDLEPLHDIYLDRVKELMAKGITP
                     DMVQSMVKTWVPMGESGLGLVQSLLGQFGGGLMGGAKPTDTADDGKPAKGRKS"
BASE COUNT         1595 a         2745 c         2872 g         1665 t
ORIGIN      
        1 ggtgagcgcc tcttcgtcga catgcgccgc gctcgtctcg aaatcgagcc cggcggcgcg
       61 gagcatcgcg gcgcggccgc tgctttgcga ggcgaggagc agggtcatgc ggcgctgcgg
      121 tcttcgacga gcttgatcac cgcggcggcg gtttcctcga tcgagcggcg cgtgacgtcg
      181 atcaccggcc agccattgtc ggcgaacatc cgccgcgcat aggcgagctc ggccttcacc
      241 cgctcgtcgt caacatagct cgtctcgggc gcctggttca aagacaggag gcggttgcgg
      301 cggacctgca cgagccggtc gagcccggtc gtcagcccca cgatcatcgg acgcttcagc
      361 gtgtacagcg cgctcggcgg cggcgattcg gggacgatcg ggatattcgc ggtcttgaag
      421 ccgcggttcg cgagatagat gctcgtcggg gttttcgagg tgcgcgacac gccggcaagc
      481 acgatgtcgg cctgctccca attttcccag ccaatgccgt cgtcatgcgc gacggtaaat
      541 tgaatggcct cgacgcgcgc gaaataggcg gcatcgagcg catgctggcg gccgggacgc
      601 gccttcgctt cctgcccgag catccgcgac agcgcgtcgg tcacggcgtc gagcgccgca
      661 accgtcggca ggttgagcgc tgtcgcgcgg cgttcgaggc tgacgcgcag ctcgccattc
      721 accagcgtga agaggatcat gccggggctt gcctgcactt cggccatgat ccggtcgaga
      781 tgcgattccg atcgcaccat cggccagaaa tggcgcacga cctcgacatc gtcgaactgc
      841 gcgatcgccg ccttggcgat attttcgagt gtctcgcccg tggagtcgga tatgagatgt
      901 aagtggagcc ggcccatcgc ggcaccatgg cgggaaactg ccgaaaaagg caatcccgga
      961 tcaagtccgg gatgacgaat agagccctgt ggataatttt tggagaagcg cggggagcaa
     1021 atcgggacga tcgattcatc cccgaccgtg tcgattccgc tccatcgctt atccacaagg
     1081 gacgaatctt atccacaggc tgtgaatagc gggataagcg aggtcgactc tgcggcgggc
     1141 ggcggcggct gtggagtcaa gctgttggca aaaaggacgc cccggcctaa agccgcgctt
     1201 gtccgccgac caacaaacca ccacaatttc atttaagatt atattgagaa gagaaagagg
     1261 gctcgcgtga aggcgtcacc gaagaagctt ctggcaacgc tgcgcggggc gcgtcaggag
     1321 cgcccgccgc tctggctgat gcgccaggcc gggcgctatc tgcccgaata tcgtgccttg
     1381 cgcgaaacca agggcggctt cctcgagctt tgctacgatc ccgaggcagc cgccgaagtg
     1441 acgttgcagc cgatccggcg cttcggtttc gacgggtcga tcctcttttc ggacattctc
     1501 gtgatcccgc acgcgctcgg ccagcatttg tggttcgagg cgggtgaggg accgcggctc
     1561 gccccgccgc tcgtcgatag cgccctggcg tcactcgaag cggcgcacca gcggctcgat
     1621 ccggtttatg cgacggtgac gcgcgtcgcg gcctcgctcc cgtctgaaac gaccttcctc
     1681 ggctttgcag ggagcccgtg gacggtcgca acctatatgg tggcggggca ggggtcgaag
     1741 gatcaggcta ccgcccggcg gctcgcgttc ggcgatccag cggcgttcca ggcgattatc
     1801 gacgcgatcg tcgagctgac ggtcacctat ctgtcgggtc agatcgaaaa tggcgtcgac
     1861 gcagtgcagt tgttcgacag ctgggcggga agcctgtcgc cggcgcaata tgagcaatgg
     1921 gtgatcgcgc ctaacgccga gatcgtgcgc cgattgaggg cgctgcatcc ggacacgccg
     1981 atcatcggct ttccaaaggg tgcgggcggc aagctccgcg cctatgccga cgagaccggg
     2041 gtcgacgcga tcgggctcga cgaaacggtc gatccgctgt gggccgacgc catcctgccg
     2101 gcgcatcttc ccgtgcaggg caatctcgat ccgctcgcgc ttgtcgccgg cggcgaagcg
     2161 ctcgataccg cgatcgatcg aatccttgcg gccttcccgt cgcgtcccca tatcttcaac
     2221 ctcggccatg gcatcgtgcc cgacacgccg atcgcgcatg tcgagcatct gatcaggcgc
     2281 gttcgcggcg gataatctat ggaactggct ggttttttgg gggcgacgat gctttggatc
     2341 aaagccgcgc atatcatttt cgtgattttc ctgatggccg gcttgttcat gatgccgcgc
     2401 tttttcgtct atcaccatca atgtccggtc ggatccgacg aggacaagaa atggatcgag
     2461 cgcgaagacc ggctgcggcg gatcatcttg aatcccgcgc tgatcatcgt ctggatcttc
     2521 gggctgatgc tcgccttcaa cggcgattat tggcacgagg gatggtttat cgccaaattc
     2581 ctgctcgtga tcgcgctgtc gggctatcat ggctggatga tcggctatgc gaagaaactc
     2641 gcgcgcggcg agcgtccgct ctccgaaaag cggctgcgga tgctcaacga agttcccggg
     2701 gtcgccgcgg cgatcatcgt tatcctcgtc gtggtgcgtc cctgaatccc tatccttcgg
     2761 gcgctgcccg attgacttga ccccggcccg gttatagggg ccgatcaacc ccgctccgcg
     2821 cgggcggcgc ttccttgcgc cgaagctatc ttgaacggtc gtcccacggc cggataggca
     2881 ccccagggtg catcgaaatc gactgttatt ctctcctgaa aatctacctg gaatccatcc
     2941 atgcatctta aagaacttaa aaccaaagcc cccgcccagc tcgtcgaaat ggctgaagag
     3001 ctgggggtcg agggcgcatc gacgctgcgc aagcaggacc tgatgttctc gatcctgaaa
     3061 gaactcgccg aagagggcga ggaaatcatc gggtcgggca cgatcgaggt cctcccggac
     3121 tcgttcggtt tcctgcgttc gtccgaagcc aattatctcg ccggtcccga cgacatctat
     3181 gtctcgccga accaggtccg caaatatggc ctgcgcaccg gcgacaccgt cgaaggcgag
     3241 atccgcgcgc cgaaggacgg cgaacgctat ttcgcgctga ccaagctgat caaggtcaat
     3301 ttcgacgatc ccgaagcggt gcgccaccgc gtcaatttcg ataacttgac gccgctctat
     3361 ccgaaccaga agctgtcgct cgacaccgtc gatccgaccg tcaaggacaa gtcggcgcgc
     3421 gtgatcgacc tcgtcagccc gcagggcaag ggccagcgcg cgctgatcgt cgcgccgccg
     3481 cgtaccggca agacggtgct gctgcagaat atcgccaagg cgatcaccga caaccatccc
     3541 gaggtttacc tgatcgtcct cctcgtcgac gagcgtcccg aggaagtcac cgacatgcag
     3601 cgcagcgtga agggcgaagt cgtctcgtcg accttcgacg aacccgcgac acgccacgtc
     3661 caggttgccg aaatggtgat cgaaaaggcc aagcgcctcg tcgagcacaa gaaggatgtc
     3721 gtcatcctgc tcgactcgat cacgcgcctc ggccgcgcct acaacaccgt cgttccgagc
     3781 tcgggcaagg tgctgaccgg cggtgtcgac gccaacgcgc tccagcgccc caagcgcttc
     3841 ttcggcgccg cgcgtaacat cgaggaaggc ggttcgctct cgatcatcgc gaccgcgctg
     3901 atcgataccg gcagccgcat ggacgaagtg atcttcgaag aattcaaggg cacgggtaac
     3961 tcggaaatcg tgctcgaccg caaggtcgcc gacaagcgta tcttcccgag cctcgacgtc
     4021 ggcaagtcgg gcacgcgcaa ggaagaattg ctcgtcgaga aggacaagct ttcgaaaatg
     4081 tgggtgctgc gccgcatcct catgcagatg ggcaccatcg acgcgatgga attcctgctc
     4141 gacaagatca aggattctaa gaccaacgag gatttcttcg acagcatgaa ccagtaagcc
     4201 cggatcatgg ccgtgatcga acacgccttg ttgcacgtcc gtgcgggcag cgaagacgcg
     4261 ttcgaggcgg ccgtggccga ggcacggccg ttgatcgaag catcgccggg cttcatttca
     4321 ctggaaattc gccgtccgac aggaacggga cagccttatc tgcttttagt ggagtggagg
     4381 tcggtcgcgg atcatcggga cggcttccgg caatcggatc gctatcaaca atggcgggcg
     4441 ttgctgcatc acttttatga ccctatgcca gaggtcagtt atttcggaga gccgctgtga
     4501 ttttggaaat attgacccag gctggcgctg ctggcgccca cgccgccgga tttggtggcc
     4561 ccgccgacat gtgggcggcg atcgtcaacg atttttcgaa catcaccgag cccgcggcct
     4621 ttgccgcctt tctgcaggtg ttgatgatcg acctcgtgct cgcgggcgac aatgcgatcg
     4681 tcgtcggcgc gctcgccgcg ggtcttcccg ccgaacagcg caagaaggtc atcctgatcg
     4741 gcgtgatcgc ggcattggtg ctgcgcatcg ccttcgcgct cgtcgtcacc cagctgatgc
     4801 agatcgtcgg gctgatcttt gtcggcgggc tgctgctgat ctgggtcgcg tggaagatgt
     4861 ggcgcgagct gcgccacacc ggccagtcgc atggttcgcc cgagattgcg ggtgacgagc
     4921 attcgggtct gaagcccgcg aagagcttcg ccggcgcagc ctgggcggtg gccgtcgccg
     4981 acgtcagcat gagcctcgac aatgttctcg cggtcgccgg tgcggcgcgc gagcatccgg
     5041 gcatcctgat cgtcggcctg atctttgccg tcgccttgat gggcctcgcg gcgaacatca
     5101 tcgccaaata tatcgagcgc taccgctgga tcgcctatct cgggcttgcg gtgatcgtct
     5161 atgtcgcggt caagatgatc tacgaaggct gggtcgatcc gggcgtcggg gtcggcacgc
     5221 tgttcggctg atctcttttc ggcttgaaat ggaaaggcca gctccgccca aataagggga
     5281 ggggctggcc tttttcgtgc aggcttccca tctgctttgt atgtcccagc ttcacatgac
     5341 ccgcgttgcc gtcggctgtt ccgattaccc agcgctgcaa gcgcggatcg ccaactatgc
     5401 ccagaagggc gaaatccgtt tcacgacacg cttccggccc aagcgcgccg acgaactgat
     5461 cggcggcaag ctgcatttca tcgtcaagca cacgctcgtc ggacgcgtcg agattttgcg
     5521 gttcgaggat cgcagcgacg ggcgggtcga tatcgtctgc gtcgcagcgc tcgagcggat
     5581 tcatccgcag ccgaagcgcg cgcatcaggg gtggcgttat ttggcgggca gcgatgcacc
     5641 gaagggaatt agcgaggatg acgggctcgc cgatctgccg cccgagctgt atcgcgaact
     5701 cgccgaactg agcctgatct agaaggcggc gcggaagctt tcggcgcgcg ccatcgccca
     5761 gtaacgggcg cttgcgaccg accctggatc ttcgaggaga accccgcgat cgtagaattt
     5821 atagatgcgg tctatcgagt ccgagcgcgc cgaattgagc cgttcgtgga gatggtgcgg
     5881 acggatcgcc cacggatcgt cgagccccat cgcgcagctg atatcccaca gcgccgaaac
     5941 ggtcgcggcc tggaaacggc gcacgcgttc ggccttgtcc tcgacgacga ggccgctctg
     6001 gcggccggga tcctgcgtcg cgacgccggt cggacacatg tcggtatggc atttgagcga
     6061 ctggacgcag ccgagcgaaa acatgaaggc gcgcgcggcg ttcgaccagt cggcaccgag
     6121 cgcgcaattg accgcgagcc ccgcgcccga atggaccttg cccgacgccg cgaggcgaat
     6181 ctcttccttg aggccgcagc cgacgagcgc gttgcgcacg aagatcagac cctcgcgcaa
     6241 aggcatgcca acgcggttcg aaaattcgac cggcgccgcg cccgtgcccc cctcggcgcc
     6301 gtcgacgacg atatagtcga ggcgaatgcc ggttgcgatc atcgccttca tgacggcata
     6361 gatttcgtgc ggctggccga cgcagagctt gatcccgacg ggttttcctc ccgacaactc
     6421 gcgcaatcgg gccgcccatt cgagcatctc gatcggggtg tcgaacgccg aatgcgcggc
     6481 gggcgagatg caatcctgtc cctcgggaac gccgcgcgtc gccgcgatct cagcgctgac
     6541 cttcgcgccg ggaaggacgc cgccatgccc aggcttggcg ccttggctca gcttgatctc
     6601 gatcatcctg acctgatcct cggccgcgcg gtcgcggaag cgttcagggt cgaagcgccc
     6661 ttccgcgtcg cggcagccga aatagccgct gccaagttcc cagacgatgt cgccgccgtg
     6721 ggtgcggtga taggggctga agccgccctc gcccgtgtcg tgatagaagt tgccgagccg
     6781 ggcaccgagg ttgagcgcct cgatcgcatt ggcgccgagc gaaccgaagc tcatcgccga
     6841 gatgttgagc agggccggtc gatagggttt cgaacattgc gacgtgccca ccaccgggcg
     6901 ctgcgtccgg tcgtgcccca tcaccggcgc gatgctgtgg ctcatccact catattcgtc
     6961 cgaatagaca tcgagttcgg tgccgaaggg gtggctgtcg agttcgcctt tcgcgcgtgc
     7021 atagatgata tcgcgctcgg cgcgcgcaaa ggggcggccg tcgagaggcc cttcgacgat
     7081 ataggcgtag aggaacggcc gcagatcctc gaacaaccag cgaatgcgcg cgatcagcgg
     7141 gaagtttcgg cgcaggctgt ggtgccgctg gatcgcgtcg tagatggcga cgagaaggag
     7201 cggcacccac agtatcagcg tccagcgcgc cggcgaccac catgtcgcca gcgcaatcgc
     7261 ggcaaggagg acggggaagg cgatccagcg gtgcatttga tggcgagcgg ctaacccgcc
     7321 tgcgccgcga gcattgccgc catcttttcc caaaccttgt tgatcgcctt gagcggtcgg
     7381 accatcacct tgaaatcttt tatctttccg tcttcattcc aggtgatgat gtcgaccccg
     7441 ttgatctgga tctcgtcgag attgctctgg aattcgagca tcgcttggtc gccgtcggcg
     7501 atctcgcgca aatagcggaa atcgtcgccg ccgagaacgt ggctcgcggc gtggaggtag
     7561 gcgaagacct tgtcggcgcc ttcctgcggc gtgtggacga cgggcgagtg gaacaccgca
     7621 tcctcggcga gcaggtcgcg caaggcctgc gggtcgccgc cgcccgccat ataggcgtgc
     7681 caggcggcga gccccgcatg gtccgtcatg cgagcttctc ggcgaagaag gcgcgggagc
     7741 ggccgtcggc gagctgcgcg ccttcctcgt cgcggcggtt gcccatcgtc gcggcgaagc
     7801 catggtcaag ccccggatag tcgtgcagcg tgaccttcgg atgcgggtcg agcgcctcgt
     7861 gaatcttctt ctgcgccgca tggtcgacga aatgatcctc ggttgggatg tggagcatca
     7921 gcggattggc gatcgcatgc gcttcgttca gcatctggtc gatcatgacg ccataatagc
     7981 cgaccgacgc gtcgatatcg gtgcgcgtcg ccgccatata ggcgaggcgg ccgccgaggc
     8041 agaagccgac gcatccgatc ctttcggcgc cttcgccgcg cagccagtgg atcgccgcct
     8101 cgatatcctt gacgccgtcg tcggcgtcat attggccgaa ataaccgagc gcctgctgaa
     8161 attcggcctc gatatcgggg ctgagctcga cgccgggggc gaagcgccag aagatgtcgg
     8221 gcgcgatcgc gagatagcct tcggccgccc agtcgtcgca tttcttgcgg atgccttcgt
     8281 tcaccccgaa gatttcggga atgacgatga tcgcgcgcgc gctgtcggcg tcggggcgtg
     8341 cgacataggc gggcagggta ccggcgccgt cgagcgtcgg gatattggtg ttggtcgcgg
     8401 ccatgtgggg agctctcctg tctcttgctt ctttgcggtg aaagcgcgaa gctagcggcg
     8461 cgatgagcat ggctcaagcg gctaaccggc atttttcggg gcggagacag gcgatgaagt
     8521 tcaatatcga gatcgattgc acccccgaag aggcgcgccg cctgttcggc cttcccgatc
     8581 tcgagccgct ccacgatatc tacctcgacc gcgtcaagga attgatggcg aaggggatca
     8641 cccccgacat ggtccagtcg atggtcaaga cctgggtgcc gatgggagag agcgggctgg
     8701 ggctggtcca gtcgttgctc ggtcagttcg gcggcggact gatgggcggg gcgaagccga
     8761 ccgacacggc cgatgatggc aagcccgcca aggggcgcaa gagctgaacc aaaatcctgt
     8821 gagcgacacg attttcgcgc tgtcgagcgg gatgccgccg gcggcgatcg gcgtcgt
//
LOCUS       DATMSU010000120         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172848,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000120 DATMSU010000000
VERSION     DATMSU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172848"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VN034_03230"
     CDS             <1..220
                     /locus_tag="VN034_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HWV59640.1"
                     /translation="AYLAAGSTRGLPPGPFYDIGLFYGGGGRLLMPFGWIQANACARI
                     VDEDLAKAQADCRTIRRNGACTLSLETA"
     gene            233..1132
                     /locus_tag="VN034_03235"
     CDS             233..1132
                     /locus_tag="VN034_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HWV59641.1"
                     /translation="MTYDDLMSLLGNWLLPGSLVAIMVSMGLSLTADDFRQVFRNKRA
                     LIFGVCSMLIVPPLIGLSLALTVAPTAALAVGFVLLATTPGGMLSNLFTDMAKGDLAL
                     SMSMTLILSMIYIFVVPFYAHFALLHFMGLEADVQVPLASFFWDIFSITVLPATVGFA
                     VRAWRPDFAIWFKRYLKNAATIVLFSSFGVILYDQVPVLRENLGALFWITVALNLIMV
                     VVVMTIVRFAGFTRRENVAIGIEHLMRQEGTAIFIAVSIVGNNEMSLPMIMNTPVALC
                     FVLLFVAIARRTGRPAQGAPATI"
     gene            1268..>1963
                     /locus_tag="VN034_03240"
     CDS             1268..>1963
                     /locus_tag="VN034_03240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV59642.1"
                     /translation="MQHRSISRAARNDSVTAPLWTATAAPGPVLAPLAGHVETDILVI
                     GGGIAGTATALHLAERGADVVLVEAGQPGDGATGQSGGLIAPDYIRHSPETIRQTLGE
                     EAGERLTQMIGASGQRVFDLVERHDIDCDARQDGFYTPAHTEALADTQRNYANQWRAR
                     GHDVDFVAGDEVRRLFGADRYCGALRFAGGGSLNPLAYVRGLAAAAVRHGARLFAGSP
                     VRSLERRNGGWVCR"
BASE COUNT          294 a          635 c          645 g          389 t
ORIGIN      
        1 cgcctatctg gcggcgggca gcaccagggg gcttcccccc ggccccttct atgacatcgg
       61 tctcttctac ggcggcggcg ggcggctgct gatgccgttc ggctggatcc aggccaatgc
      121 ctgcgcgcgg atcgtcgacg aggacctcgc caaggcgcag gccgattgcc gtacgatccg
      181 ccgcaatggt gcctgtaccc tttcgctcga aaccgcctga ggacattcgc ggatgactta
      241 tgacgatctg atgtccttgc tggggaactg gctgctgccg gggtcgctgg tcgcgatcat
      301 ggtgtcgatg gggctgtcgc tgaccgccga cgatttccgt caggtgttcc gcaacaagcg
      361 cgcgctgatc ttcggcgtct gttcgatgct gatcgtgccg ccgttgatcg gtctgtcgct
      421 cgcgctgacc gtggcgccga ccgcggcgct ggcggtcggc ttcgtcctgc tcgcgaccac
      481 gccgggcggg atgctgtcga acctgttcac cgacatggca aagggcgatc tcgcgctgtc
      541 gatgtcgatg acgctgatcc tcagcatgat ctacatcttc gtcgtcccct tttacgcgca
      601 tttcgcgctg ctccacttca tggggctcga ggccgacgtg caggtgccgc tcgcgtcgtt
      661 cttctgggac attttctcga tcaccgtgct gcccgcaacg gtcggtttcg cagtccgcgc
      721 gtggcgcccc gatttcgcca tctggttcaa gcgctacctg aaaaatgccg cgacgatcgt
      781 gctgttcagc tcgttcggtg tcatcctcta cgatcaggtg ccggtgctgc gcgaaaatct
      841 gggcgcgctc ttctggatta ccgtcgcgct caacctcatc atggtcgtgg tggtgatgac
      901 gatcgtgcgc tttgccggtt tcacgcggcg cgagaatgtc gcgatcggga tcgagcattt
      961 gatgcggcag gaaggcaccg cgatcttcat cgccgtatcg atcgtcggca acaatgaaat
     1021 gtcgttgccg atgatcatga acacccccgt cgcgctctgc ttcgtgctgc ttttcgtcgc
     1081 catcgcgcgg cgaacggggc ggccggcgca gggagctccc gccacgattt gaaacacaac
     1141 agtccggcga ccgaccgggc gagcaggaaa gttcgcatat gatattgtcc cgagttgagg
     1201 gcgtcgtgac ggggttgctc cccctcgtcg cgataccctc ccccttcgtc tggtcgacgg
     1261 agcggtaatg cagcatcgat ccatatcccg tgccgcgcgc aacgacagcg tgacggctcc
     1321 cttgtggacc gcgaccgccg cgcccggtcc cgttctggcg ccgctcgcag gccacgtcga
     1381 aacggatatt ctggtaatcg gcggcggcat cgcgggcacc gcgaccgcgc tgcacctcgc
     1441 cgaaaggggt gcggacgtcg tactcgtcga agccggacaa cccggcgatg gcgcgaccgg
     1501 acagagcggg gggctcatcg cgcccgacta tatccgccat tcgcccgaaa cgatcaggca
     1561 gacccttggc gaggaagccg gcgagcgcct gacacagatg atcggcgcgt cgggacaacg
     1621 cgtcttcgat ctggtcgagc gccacgatat cgactgcgat gcgcggcagg acggttttta
     1681 cacgcctgca cacaccgagg cgctggcgga cacccagcga aactatgcca accagtggcg
     1741 cgcgcgtggc catgacgtcg atttcgtcgc gggcgacgag gtgcggcggc tgttcggggc
     1801 ggatcgctat tgcggcgcgc tgcgctttgc aggcggcggc agcctgaatc cgcttgccta
     1861 tgtccgcggg ctggctgccg cggcggtgcg tcatggcgcg cgcctgttcg ccggcagccc
     1921 ggtgcgatcg ctcgagcgcc ggaacggcgg ctgggtatgc cgg
//
LOCUS       DATMSU010000121        12213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_59210, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000121 DATMSU010000000
VERSION     DATMSU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 12213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12213
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59210"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..799)
                     /locus_tag="VN034_03245"
     CDS             complement(206..799)
                     /locus_tag="VN034_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59643.1"
                     /translation="MAAENSEFEFCGVNHLALVCKDMAKTVEFYRDKLGMPLTKTIDL
                     PGGRGQHFFFDIGNGDSLAFFWFPNAPEAVPGISAPAALPTKGSFVSAHGSMNHIAIN
                     VRADKFDDYYNRLIEKGIEVTPVLNHDDSPTQASAEMHPGVFVRSVYFFDPDGVCLEF
                     AAWTKTFDERDVAHDPVQADGTKREGFIVGRTPVPAE"
     gene            complement(811..1428)
                     /locus_tag="VN034_03250"
     CDS             complement(811..1428)
                     /locus_tag="VN034_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWV59644.1"
                     /translation="MKFYNSVGPNPRVVKLFMAEKGIEIPEVSVDLRGGENRRAPYNV
                     EVNPAGQTPALELDDGSTLCEITAICEYLDERFPEPTLIGSTPEDRANTRMWVRRIDL
                     KICEPLTNGFRFAEGLPLFEARLRCLPDAAEGLKATAQDGIKWLDAQIAGREFVAGDA
                     FTLADIMLFTFLDFGASVGQPIDPANTNILGWFERTKSRPSAAAN"
     gene            complement(1488..2402)
                     /locus_tag="VN034_03255"
     CDS             complement(1488..2402)
                     /locus_tag="VN034_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWV59645.1"
                     /translation="MTGYGAPQVVATGLRFPEGPVPTADGSVLLVEIARGTLTRVAPD
                     GELSVVAELGGGPNGLAIGPDGAAYVCNNGGFEWIEAGGLLFPGHAANAEPCGSIQRV
                     DLSTGAVTTLYDSFEGERLKGPNDIVFDANGGFWFTDHGQVRETGRDHGAIFYAAADG
                     SRISRQRADMMGPNGIGLSPDAKTLYVSETMTARVWAMDIVASGELAPPPPWAPGRFL
                     GIPPAFRLLDSLAVEESGRVCVGTLVEGGITVFDPDGGGSEFVPLPDVGITNIAFGGP
                     DRRDAYITASTTGTLYRVRWPRPGLQLR"
     gene            complement(2410..3057)
                     /locus_tag="VN034_03260"
     CDS             complement(2410..3057)
                     /locus_tag="VN034_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWV59646.1"
                     /translation="MPVLRQVPRSEVTDETVLAYYNRLFGDRDPVAEPGTATGTPGDW
                     WTVFALSPDIFEHAVKGFAVYRNPARTIDPVLRELGQTRAGWVKGSQFVFSQHCKSLR
                     GLGVSEEKIAAIAHWQVADCYDEQERAVLAYADCLAQAGGRVPLEVFDKLKTFWNDEQ
                     IFEFTYITCLYDMHAVITRALRMEYDAREDPIVEVAAPEGFSAADFLSAPRPAQG"
     gene            complement(3068..3463)
                     /locus_tag="VN034_03265"
     CDS             complement(3068..3463)
                     /locus_tag="VN034_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59647.1"
                     /translation="MAEQDDIRAMAQRFFDAIEAGDIETMQGSFTPNAEIWHNTDELI
                     VTPAQTAQTLTGMVARIKDREYADRQLTTFPGGFVQQHVLKGKRVHDDGAVRLPCAII
                     CKVENGKITRLDEYFDSAHVAEFRKFANA"
     gene            complement(3474..4361)
                     /locus_tag="VN034_03270"
     CDS             complement(3474..4361)
                     /locus_tag="VN034_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59648.1"
                     /translation="MTDVAGKTAFITGGASGLGLATAKALAAKGASVILADIDGAGAD
                     AAAEALRSDRANALGLQLDVTSEESWAEAGAKARDFGPVRILFSNAGVGGGSGPFEQY
                     DTDVWRWNYAVNAHAHLYACRTFLGDMKTSGEPSHLVITSSMVAIVPPPISVAYISSK
                     YATLGIAMALRNELAETCVDISVLMPGMSATRIVETTRDLRPVEVEVGKAAATSQAMQ
                     GVLAGGMSPDRIGARVVQAIEAGEYWIFTHPEWKAMAELVTQDMLSSFGPSADPAYKG
                     DDIDGLIAANGGRMFGAKV"
     gene            complement(4358..5653)
                     /locus_tag="VN034_03275"
     CDS             complement(4358..5653)
                     /locus_tag="VN034_03275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59649.1"
                     /translation="MTEATAFSAGTAAPDNGHALRRNVALAMLFVVGTINFVDRQLLS
                     VLVEPIRAEMDFSDTQFGLLTGFAFALFYAAAGVPVAMIADRWNRVKLIGIACVVWSG
                     FTAACGMVSNFWQLALMRFGVGAGEAGGTAPSLSVIADYYPPARRPLAIGLFTLNGPF
                     GVFVGATFGAWAAANIGWRNAFIVIGIVGILVAPLLIWLVREPARGAMDTHKPADEAL
                     PFSQSLAMFIRRPSLLMVMIGSGLAAFVSYGMLNWIPAFLMRTQKMPLEAMATWFGPA
                     AGITFGIGILGGGWLVSHRAKHSARAYGTIPALATAVLIPTFIAALLVDTWQLSLALM
                     LIPMAACTAYVAPALALVQNLTPPRSRATAAAVLMLMFNIIGLGLGPLFAGVVSDSLK
                     PAYGDDSLRWALMALIPFAAAAGLAQYRMTRYLEKDFAE"
     gene            5762..6529
                     /locus_tag="VN034_03280"
     CDS             5762..6529
                     /locus_tag="VN034_03280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59650.1"
                     /translation="MDIKGKTAIVTGSAGGIGRATAVMLAERGAAEIRLVDVKEDALA
                     ETARLVAAKGARVTTHVLDLSDIAVVEAWFEAHGEVDILHNNAGVVSGLPQFPDVDAA
                     RIRWIIDVNITSLVAATQIAVQKMKARGGGVIINTVSTVALGTGFYDAMYATTKAAVM
                     MFTRCCAPLKEECNVRVAGMLPGLVDTPILDTTGAGGKSEWMKTVLANNEACRPEDIA
                     GGVMALIEDDSLAGGDWVAVRRLEGKVEYQWGHADTV"
     gene            complement(6526..7236)
                     /locus_tag="VN034_03285"
     CDS             complement(6526..7236)
                     /locus_tag="VN034_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWV59651.1"
                     /translation="MIDVYFTPTPNGHKVSIMLEEVGLPHQLLAMNMLEGDHLTPEYR
                     RINPNGRLPAIVDHDPIGGGAPLPVFESGAILLYLAEKSGQLLPADPRRRSQAQQWLM
                     WQMASFGPMQGQAHHFIRYAPEGQAYPVERYRNETLRLLHVLNGRLAQTDYLAEEYSV
                     ADIACWPWTRAIRAIGLTLDDYPAVADWFARICERPAVIAGTDVKNAANLSSARPVLT
                     EEQWSNLFGKNMLEAPTR"
     gene            complement(7337..9604)
                     /locus_tag="VN034_03290"
     CDS             complement(7337..9604)
                     /locus_tag="VN034_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59652.1"
                     /translation="MKAHNQFLLSGVAAIAMLANAAPAMAQAAPADAAPVDTAEVDDS
                     SSNEIIVTAQKREQSLQDVPISMEVVSGAKLAEFNTSDIKAVMNYTPNVFVQSTAGND
                     VIYIRGFGSPPANFAFDQSVSLYVDGIYAGRNRQAQAPFFDLERVEVLRGPQGALFGK
                     NTAAGAVSVVSAGPTKDFEGAITGLYNFDHKGTDFSGYLSGPITDTLGARFAYKIVNQ
                     DGYIYNRAKDHDDPEIKSQLLRLTLKWEPSANFDYTAKVEYGNREVIGGITVSSPLTS
                     DQDPQTNRYLERSALGDEGNDNKSVMISGVGNLALGDFTLTSVTGYSWFKSKIVNGFD
                     QTIPNSGGAFTANSVYNAFPERFDQFSQEIRILSPVGETFEFIGGAYYDKSNYTLEQY
                     QGFNIPNLNIPGVFTGPYFGRIDSIFNQDAESWSVFGQGTFNATEALRVIGSLRYTHT
                     KKDGDFAARLVYGPFAIRPISSAEGSISEGNVDPSVTVQYDIAPRIMVYATWGRGSKS
                     GGFVSNTLGTVDSTFTFEPEQSENFEAGIKSTLFDGKVVANVSAYHTQFKDLQVSVYQ
                     PATSSYLTGNAASATSKGIEGSLSVFPIPNFDISASAAYSDIKYDDYPGAACLASQPA
                     TCTPATNNLAGYPVAYASKWTGSVTAHARFDMSDALKLDITGVAAGRSKYFNSDNQSP
                     VFGVQNGYVKLDLRVQLADQDDRWHVALVGKNLTNQKTIGSAFNLPFPITPVPRAILY
                     LEPTRNISVEAGIKF"
     gene            9727..10233
                     /locus_tag="VN034_03295"
     CDS             9727..10233
                     /locus_tag="VN034_03295"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HWV59653.1"
                     /translation="MNEEISHGVSTALATRHLCTVEFEVGGGLIGIGASPFGDQRLGY
                     ISGGRFFGPRINGIVLPGGGNWSRSGRLGDSASVGTFDARAVWQTDDGALIYLSYTGR
                     NIIPDDVRATFADPAVPDADPSRYYLRIAPVFETASAKYGWLNGVLAVGVGERTDFGV
                     RHVIHEVL"
     gene            10235..10960
                     /locus_tag="VN034_03300"
     CDS             10235..10960
                     /locus_tag="VN034_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWV59654.1"
                     /translation="MIDLHYSATPNGQKIAIMLEEIGEPYRVIPYDIFDGDQLTAEFG
                     RINPNHKLPAIVDQHPADGDDPVTVFESGAILQYLAEKSGRFLPPSGAARAATLSWLT
                     WQVAGLGPMGGQASHFLRYAPAGQDYAVERYTKELTRLLTVLERRLEKSAYVAGDDYT
                     IADMAIWPGRASAFVMGMGLDDWPATRRWFEQIRGRPAVARAMAREDLKAPAKYVGRH
                     QTLDEKEWSNMFGDANHAAVKGD"
     gene            complement(11164..11868)
                     /locus_tag="VN034_03305"
     CDS             complement(11164..11868)
                     /locus_tag="VN034_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWV59655.1"
                     /translation="MITLYGGPTPNARKIAIALLDMELDWRLEYIDILAGDQLTPEFL
                     ALNPNNKTPVIVDEEGPGGERFVLWETGAILLYLAEKTGRFLPTDPVARATCWQWLMF
                     QVSGVGPMFGQEAHFTHYAKDRHDYAIARYSREVDRLMMVLDKRLGEAEWLAGDDYTI
                     ADMATLPYLRRQLIEKAGRFPNVDRWGAAMLERPAVAEGMTVGVARAETIEGGLTGFT
                     DEHRAILWGDRQHVKR"
     gene            complement(11873..>12213)
                     /locus_tag="VN034_03310"
     CDS             complement(11873..>12213)
                     /locus_tag="VN034_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWV59656.1"
                     /translation="ATHRARGARQAELDALIGEWTATLTIAELEAKMIEAGVPAGRVF
                     DAQDMLADPHFAARDALVTVADEEFGEVTMQGVFPKLSATPGSVRRPAPLTVGQDGAE
                     VLKRWLGRET"
BASE COUNT         2167 a         3765 c         4067 g         2214 t
ORIGIN      
        1 ggggttaggg gtgggtgcgc gagcgcagcg agcaagaaag aagaacgccg ccttcggcgg
       61 ctacccaccc ccggcccctc cccagaaggg aggggagaag gtaagtccgc ttttcggtcg
      121 aaagcggccg atcaccaccc ccaaaaaaga acccggccaa cggccgggtc caggaggagt
      181 tcccagtggt gtcttcggga atcttctatt cggcgggcac cggggtccgg ccgacgataa
      241 agccttcgcg cttcgtgccg tcggcctgca ccgggtcgtg cgcgacgtcg cgttcgtcga
      301 aggtcttggt ccatgccgcg aattcgaggc agacgccgtc ggggtcgaag aaatagaccg
      361 agcggacgaa tacgccgggg tgcatctcgg ccgacgcctg cgtcgggctg tcgtcgtggt
      421 tcagcaccgg cgtcacctcg atgcccttct cgatcaggcg gttgtaataa tcgtcgaact
      481 tgtcggcgcg gacgttgatc gcgatatggt tcatcgaccc gtgcgccgac acgaagctgc
      541 ccttggtcgg cagcgcggcg ggcgccgaga tgcccgggac cgcttcgggc gcgttcggga
      601 accagaagaa ggcgaggctg tcgccattgc cgatgtcgaa gaagaaatgc tggccgcgcc
      661 cgccgggcag gtcgatcgtc ttggtgagcg gcatgccgag cttgtcgcgg tagaattcga
      721 ccgtcttcgc catatccttg cacacgagcg cgaggtggtt gaccccgcaa aattcgaatt
      781 cgctattttc tgcagccatc atccgtctcc ttagtttgcg gcggcgctgg ggcggctctt
      841 cgtccgctcg aaccagccga gaatattcgt gttggcgggg tcgatcggct ggccgaccga
      901 tgcgccgaaa tcgagaaatg tgaacagcat gatgtcagcg agcgtgaagg cgtcgccagc
      961 gacgaactcg cgtccggcga tctgcgcatc gagccatttg atcccgtcct gcgccgtcgc
     1021 cttcagcccc tcggccgcgt cgggcaggca gcgcagccgt gcttcgaaca aagggagccc
     1081 ttcggcgaag cggaagccgt tggtcagcgg ttcgcaaatc ttgaggtcga tgcggcgcac
     1141 ccacatgcgc gtgttggcgc gatcctcggg agtcgaaccg atcagcgtcg gctcgggaaa
     1201 gcgttcgtcg agatattcgc agatcgcggt gatttcgcag agcgtgctgc cgtcgtcgag
     1261 ttcgagcgcg ggcgtctgcc cggcgggatt cacctcgaca ttatagggcg cacgccggtt
     1321 ctcgccgccg cgcaggtcga cgctgacctc gggaatctcg ataccctttt cggccatgaa
     1381 cagcttcacg acgcgcgggt tggggccgac actattatag aatttcattt ctatcctctc
     1441 acgcgagtct tatcattttt cggcataatc gtcaattaaa ttgacggtta gcgaagctgt
     1501 agccccgggc gtggccagcg gacgcgatag agcgtgccgg tggtcgaggc cgtaatataa
     1561 gcatcgcgcc ggtcggggcc gccgaaggcg atgttggtga ttccgacatc ggggagcggc
     1621 acgaactccg atccgccgcc atcggggtcg aacaccgtga tcccgccctc gaccaaggtg
     1681 ccgacgcaga cgcgtccgct ttcctcgacg gcgaggctgt cgagcaggcg aaaggcgggc
     1741 gggatgccga ggaagcggcc cggcgcccag ggcggcggcg gggcgagttc gccggacgcg
     1801 acgatgtcca tcgcccagac gcgcgcggtc atcgtctcgc tgacgtagag cgtcttggcg
     1861 tcgggcgaca ggccgatgcc gttcggtccc atcatgtcgg cgcgctggcg gctgatccgc
     1921 gatccgtcgg cggcggcgta aaagatcgcg ccatggtcgc gcccggtttc gcggacttgt
     1981 ccatgatcgg tgaaccagaa gccgccgttg gcgtcgaaga cgatgtcgtt cggacccttg
     2041 aggcgctcgc cctcgaagct gtcgtaaagc gtggtcaccg cgccggtcga gaggtcgacg
     2101 cgctggatcg atccgcacgg ttcggcgttt gcggcgtggc cgggaaagag caggccgccc
     2161 gcctcgatcc actcgaaccc gccattgttg cagacatagg ccgcgccatc ggggccgatc
     2221 gcgagcccgt tcggcccgcc gccgagttcg gcgacgaccg aaagttcgcc atcaggggcg
     2281 acgcgcgtca gcgtgccgcg cgcgatctcg acgagcagca ccgagccgtc ggccgtcggc
     2341 accggacctt cgggaaagcg caggccagtc gcgacgactt gcggggcgcc gtagccggtc
     2401 atccgcccct tatccttggg ccgggcgcgg cgcgctgagg aagtcggcgg cgctgaagcc
     2461 ttcgggcgcg gcgacctcga cgatcggatc ctcgcgggcg tcatattcca tgcgcagcgc
     2521 gcgcgtgatc accgcgtgca tgtcatagag gcaggtgatg taggtgaatt cgaaaatctg
     2581 ctcgtcgttc cagaaggtct tgagcttgtc gaaaacctcg agcgggacgc gtccgccggc
     2641 ctgcgcgagg cagtcggcat aggcgagcac cgcacgctcc tgctcatcat agcaatcggc
     2701 gacctgccag tgcgcgatgg cggcgatctt ttcctcactg acgccgagac cgcggagcga
     2761 tttgcaatgc tgcgagaaga cgaactggct gcccttgacc cagcccgcac gcgtctggcc
     2821 gagctcgcgg agcacgggat cgatcgtgcg cgccgggttg cgatagacgg cgaagccctt
     2881 gactgcatgt tcaaagatgt cgggcgatag cgcgaagacc gtccaccagt cgccgggcgt
     2941 gccggtcgcg gtgccgggtt cggcgacggg gtcgcggtcg ccgaacaggc ggttgtagta
     3001 agcgaggacg gtctcgtcgg tgacttccga gcggggaacc tggcgcagaa cgggcatggg
     3061 cgactcctca ggcgttcgca aatttgcgga attcagcgac gtgggcgctg tcgaaatatt
     3121 cgtcgagccg tgtgatcttg ccattctcga ccttgcagat gatcgcgcag ggcaggcgga
     3181 ccgcgccgtc gtcgtggacg cgcttgccct tcaacacatg ctgctggacg aaaccgccgg
     3241 ggaaggtcgt gagctggcgg tcggcatatt cgcggtcctt gatccgcgcg accatcccgg
     3301 tcagcgtctg tgcggtctgc gccggggtga cgatcagttc gtcggtattg tgccagatct
     3361 cggcgttcgg ggtgaagctg ccctgcatcg tttcgatgtc gcccgcctcg atcgcgtcga
     3421 aaaagcgttg cgccattgcg cggatgtcgt cctgctctgc catgttattc tccttaaacc
     3481 ttggcgccga acatgcggcc gccgttggcc gcgatcagtc cgtcgatatc gtcgccctta
     3541 tacgccggat cggccgaggg gccgaaggac gacagcatat cctgcgtcac cagctcggcc
     3601 atcgccttcc attcggggtg ggtgaagatc caatattcgc ccgcctcgat cgcctgcacg
     3661 acgcgcgcgc cgatcctgtc gggcgacatg ccgccggcga gcacgccctg catcgcctgg
     3721 ctcgtcgccg ccgccttgcc gacctcgacc tcgaccgggc gcaagtcgcg ggtcgtctcg
     3781 acgatgcgcg tcgccgacat gccgggcatc agcaccgaga tgtcgacgca ggtttcggca
     3841 agctcgttgc gcagcgccat cgcgatgccg agcgtcgcat atttggagct gatataggcg
     3901 accgagatcg gcggcggcac gatcgcgacc atcgatgagg tgatgacgag gtggctgggc
     3961 tcgccgcttg tcttcatgtc gccgaggaag gtgcgacagg catagagatg cgcgtgcgcg
     4021 ttgacggcgt agttccagcg ccagacgtcg gtgtcatatt gctcgaacgg ccccgatccg
     4081 ccgccgacgc cggcgttgct gaacaatatg cgcaccgggc cgaaatcgcg cgccttggcg
     4141 ccggcctcgg cccagctttc ctcgctggtg acgtcgagct ggaggccgag cgcattcgcg
     4201 cggtcgctgc gtagggcttc ggcggcggca tcggcacctg cgccatcgat atcggcgagg
     4261 atcaccgaag cgcctttcgc ggcgagggcc ttggcggtgg cgaggccgag tccgctcgcg
     4321 cccccggtga tgaaggcggt cttgcccgcg acatcggtca ttcggcgaaa tccttctcaa
     4381 gataacgcgt catgcgatac tgggcgagac ccgcggcggc ggcgaagggt atcagcgcca
     4441 tcagcgccca acgcaggctg tcgtcaccat aagcgggctt cagactatcg ctgaccacac
     4501 cggcgaacag cggaccgagc cccagcccga tgatgttgaa catcagcatc agtacggcgg
     4561 cggcggtcgc gcggctgcgc ggcggggtga ggttctgcac cagcgcgagc gcgggggcga
     4621 cataggcagt gcacgccgcc atcgggatca gcatgagcgc gagcgagagc tgccatgtgt
     4681 cgaccagcag cgcggcaatg aaggtcggga tcagcaccgc ggtggcgagc gccgggatcg
     4741 tgccataggc gcgcgccgaa tgcttggcgc ggtggctgac gagccagccc ccgccgagaa
     4801 tgccgatgcc gaaggtgatg cccgcggctg gaccgaacca ggtcgccatt gcttccaacg
     4861 gcatcttctg cgtccgcatc aggaaggcgg ggatccagtt gagcatgccg tagctcacga
     4921 aggcggcgag cccgctgccg atcatcacca tgagcagcga tgggcggcgg atgaacatcg
     4981 cgaggctttg gctgaagggc agcgcctcgt cggccggctt gtgcgtgtcc atcgccccgc
     5041 gcgcgggttc gcgaacgagc cagatcaaaa gcggcgcgac gaggatgccg acgatcccga
     5101 tgacgatgaa ggcgttgcgc cagccgatat tcgcggccgc ccacgcgccg aaggtcgcgc
     5161 cgacgaacac gccgaacggg ccgttgagcg tgaacagacc gatcgcgagc gggcgccgcg
     5221 cgggcggata ataatcggcg atcaccgaaa gcgacggcgc cgtcccgccg gcttcacccg
     5281 cgccgacgcc gaagcgcatc aaggccagtt gccagaagtt cgagaccatc ccgcacgccg
     5341 cggtgaaacc gctccacacg acgcaggcga tgccgatcag cttcacccgg ttccagcggt
     5401 cggcgatcat cgccaccggc acgccggcgg cggcatagaa gagcgcgaac gcgaagcccg
     5461 tgagcagacc gaactgcgtg tcgctgaaat ccatttcggc gcggatcggc tcgacgagca
     5521 ccgacagaag ctggcggtcg acgaaattga tcgtgccgac gacgaacagc atcgcgagcg
     5581 cgacgttgcg gcggagcgca tggccgttgt cgggcgctgc ggtgcctgca ctaaaggcgg
     5641 tggcctcggt cataactctc cccaaaacgc acgagtcttc tcgctcgaaa aatacttagg
     5701 tatttaagtt tatctgtcaa tgtgctttac ggtaaccgca aaatcgacgg gagagaaggt
     5761 gatggatatc aagggaaaga ccgcgatcgt cacgggctcg gcgggcggca tcggccgcgc
     5821 gaccgccgtg atgcttgcgg aacgcggcgc ggcggaaatc aggctcgtcg atgtcaagga
     5881 agatgcactc gccgaaaccg cgcggctcgt cgcggcaaag ggcgcgcggg taacaaccca
     5941 tgttctggac ctttccgaca tcgcggtggt cgaagcctgg tttgaggcgc atggcgaagt
     6001 cgatatattg cacaacaacg ccggggtcgt atcgggcctg ccgcagtttc ccgacgtcga
     6061 tgccgcgcgc attcgctgga tcatcgacgt caacatcact tcgctcgtcg ccgcgacgca
     6121 gatcgcggtg cagaagatga aggcgcgcgg gggcggggtg attatcaata ccgtgtcgac
     6181 cgtcgcgctc ggcaccggct tttacgacgc gatgtacgcg acgaccaaag cggcggtgat
     6241 gatgttcacg cgctgctgcg cgcctttgaa ggaggagtgc aatgtgcgcg tcgcgggcat
     6301 gctgccgggg ctggtcgata cgccgatcct cgacacgacg ggcgcgggcg gcaagtccga
     6361 atggatgaag acagtgctcg cgaacaacga agcgtgtcgg cccgaggata tcgccggggg
     6421 cgtcatggcg ctgatcgagg acgatagcct cgcaggcggc gactgggtcg cggtgcggcg
     6481 gctggagggc aaggtcgaat atcagtgggg ccacgccgac actgtctagc gcgtcggcgc
     6541 ttccagcatg ttcttgccaa acaaattcga ccattgctcc tcggtgagca ccgggcgcgc
     6601 gctcgacagg ttggcggcat ttttgacgtc ggtcccggcg atcaccgccg gccgctcgca
     6661 gatacgcgcg aaccagtcgg cgacggcggg ataatcgtcg agcgtcagcc cgatcgcgcg
     6721 gatcgcgcgc gtccacggcc agcaagcaat gtcggcgacc gaatattcct cggccagata
     6781 gtcggtttgg gcgagccgcc cgttgaggac gtggagcagg cgcagcgtct cgttgcgata
     6841 gcgttcgacc ggataggcct gcccctcggg cgcatagcgg atgaaatgat gcgcctgacc
     6901 ttgcatcggg ccgaagctcg ccatctgcca catcagccat tgctgcgctt ggctacgccg
     6961 gcgggggtcg gctggaagca actgcccgct cttctcggcg agatagagca ggatcgcgcc
     7021 gctctcgaac accggcagcg gcgcgccgcc gccgatcgga tcatgatcga cgatcgcggg
     7081 cagacggccg ttcggattga tgcggcgata ttcgggggtc agatgatcgc cctcgagcat
     7141 gttcatcgcg agaagctggt gcggcagacc cacctcttcg agcatgatcg aaaccttgtg
     7201 cccgttgggc gtgggggtga aatagacgtc gatcatgccg cggttcaccc ctttaaacag
     7261 gtcacgaccg cggccgccgc ttccctgtcg aggaaagctt tggccgcggc cgatccctgg
     7321 gacatcataa tcgaacctag aacttgatcc cggcctcgac cgaaatatta cgcgtgggtt
     7381 cgagatagag gatcgcgcgc ggaaccggcg tgatcgggaa aggcaggttg aacgcgctgc
     7441 cgatcgtttt ctggttcgtc agattcttgc cgacgagcgc gacatgccag cgatcgtcct
     7501 gatcggcgag ctggacgcgc aggtcgagct tcacataacc gttctgcaca ccgaacaccg
     7561 ggctctgatt gtccgaattg aaatatttcg accggccggc ggcgacaccg gtgatgtcga
     7621 gcttcagcgc gtccgacatg tcgaaacgcg catgcgcggt cacgctgccc gtccatttcg
     7681 acgcataggc gactggatag ccggcgagat tgttcgtcgc cggggtacag gtggccggct
     7741 gcgaggcgag gcacgctgcg cccggataat cgtcatattt gatatccgaa taggcggccg
     7801 acgcgctgat gtcgaaattg ggaatgggga agacgctgag cgatccctcg atacccttcg
     7861 acgtcgcgct cgccgcgttg cccgtcagat agctcgacgt cgcgggctga tagaccgaga
     7921 cctgcaggtc cttgaactgc gtgtgatagg ccgaaacgtt cgcgaccacc ttgccgtcga
     7981 acaaggtcga cttgatgccc gcctcgaaat tctccgactg ttcgggttcg aaagtgaagg
     8041 tgctgtcgac ggtgccgagc gtgttcgaaa cgaaaccgcc cgatttcgac ccgcggcccc
     8101 atgtcgcata gaccatgatg cgcggcgcga tgtcatattg cacggtcacc gacgggtcga
     8161 cattgccttc gctgatcgat ccttcggcgc tcgaaatcgg gcgtatggcg aaggggccat
     8221 agaccagtcg cgccgcgaaa tcgccgtcct tcttggtgtg ggtatagcga agactgccga
     8281 tcacgcggag cgcttcggtg gcgttgaagg tgccctgtcc gaacaccgac cagctttcgg
     8341 catcctgatt gaagatgctg tcgatgcggc cgaaataggg gcccgtgaac acgcctggaa
     8401 tattgaggtt ggggatgttg aagccctgat attgctcgag cgtgtaattc gacttgtcgt
     8461 agtaagcgcc cccgatgaac tcgaaggttt cgccgaccgg cgacaggatg cggatttcct
     8521 gcgagaattg gtcgaagcgt tcggggaagg cattatagac cgaattggct gtaaaggcgc
     8581 cgccgctgtt cgggatcgtc tggtcgaagc cgttgacgat cttcgacttg aaccagctgt
     8641 agcccgtgac cgaagtcagc gtgaaatcgc cgagcgcgag gttgccgacg cccgagatca
     8701 tcaccgactt gttgtcgttg ccctcatcgc cgagcgccga gcgttcgaga tagcgattgg
     8761 tctgcgggtc ctggtcgctg gtcagcgggc tcgacacggt gatgccgccg atgacctcgc
     8821 gattgccata ttcgaccttc gcggtgtaat cgaaattggc cgacggctcc cacttcagcg
     8881 tcaggcgcag cagctgcgac ttgatttcgg ggtcgtcgtg atcctttgcg cggttataga
     8941 tatagccgtc ctgattgacg atcttgtacg cgaagcgcgc gccgagcgtg tcggtaatcg
     9001 gtcccgacaa atagcccgaa aagtcggtgc ccttatggtc gaaattatag aggccggtga
     9061 tcgcgccctc gaaatccttg gtcgggcccg ccgacacgac gctgaccgcg cccgccgcgg
     9121 tgttcttgcc gaacagggcg ccttggggac cgcgcagaac ctcgacgcgt tcgaggtcga
     9181 agaatggggc ctgcgcctga cggttgcggc cggcatagat gccgtcgaca tagagcgaaa
     9241 ccgactggtc aaaggcgaag ttggccggcg gcgagccgaa gccgcggata tagatgacgt
     9301 cgttgcccgc ggtcgactgg acgaagacgt tcggcgtgta gttcatcacc gccttgatgt
     9361 cgctcgtgtt gaactcggcg agcttggcgc cgctgacgac ctccatcgag atcggcacgt
     9421 cctgcaggct ctgctcgcgc ttctgcgcgg tgacgatgat ttcgttgctg ctgctgtcgt
     9481 cgacttcagc cgtatcgacg ggcgccgcgt cggcaggcgc cgcctgcgcc attgccggcg
     9541 cggcattggc aagcattgcg atcgccgcca cgccggacag caggaactgg ttatgcgcct
     9601 tcatcttcac cctcccatag agcttgtcgt cgcggttccg gaaagacttc cgatccgtca
     9661 atctgattga cccttagtat actaatggtt ttatgcttgt ccataggcag gaagaggatt
     9721 cgcatcatga acgaagaaat ttcgcatggg gtgtcgacgg cgctcgcaac gcgccatttg
     9781 tgcaccgtcg agttcgaagt ggggggcggc ctgatcggca tcggcgcatc cccgttcggc
     9841 gatcaacggc tcggctatat cagcggcggg cgttttttcg gaccccggat caacgggata
     9901 gttttgcccg gtggcggcaa ctggtcgcgc agcgggcggc tcggcgacag tgcttcggtc
     9961 ggcaccttcg atgcgcgcgc ggtgtggcag accgatgacg gcgccctcat ctatctgagc
    10021 tataccggtc gcaacatcat tcccgatgat gtgcgcgcga ccttcgccga tcccgcggtg
    10081 cccgacgccg atccgtcgcg ctactatctg cgaatcgccc cggtgttcga aaccgcgagc
    10141 gcgaaatatg gctggctcaa cggcgtgctc gcggtcggcg tcggcgagcg caccgatttt
    10201 ggggtgcgcc atgtgatcca cgaggtgctt tgaaatgatc gaccttcatt attcggcgac
    10261 cccgaacggg cagaagattg cgatcatgct cgaggaaatt ggcgagccct accgcgtcat
    10321 tccctacgac atcttcgatg gcgatcagct gacggcggag ttcggccgca ttaatccgaa
    10381 ccacaagctc cctgcgatcg tcgatcagca tcccgcagac ggcgacgacc cggtgaccgt
    10441 cttcgaatcg ggcgcgatcc tgcaatatct cgccgagaaa agcgggcgtt tcctgccccc
    10501 atcgggtgcg gcgcgcgcgg cgaccttgtc atggctgacc tggcaggtcg cggggcttgg
    10561 tccgatgggc gggcaggcga gccatttcct gcgctacgcc ccggcgggac aggattatgc
    10621 ggtcgagcgt tatacgaagg aactcacgcg tctgctcacc gtgctcgaaa ggcggctgga
    10681 gaaaagcgcc tatgttgccg gcgacgacta tacgatcgcc gacatggcga tctggccggg
    10741 ccgggcatcg gcgttcgtaa tgggaatggg gctcgacgat tggcctgcaa cacgccgctg
    10801 gttcgagcag attcgcgggc ggccggcggt ggcccgggcg atggcgcgcg aggatttgaa
    10861 ggcgccggcg aaatatgtcg gacggcacca gacgctcgac gagaaggaat ggtcgaacat
    10921 gttcggcgac gcgaatcacg ctgcggtgaa aggggattga ggtctgggag gtccggtttc
    10981 gaccggtagt tgccgttatt ggaatggctc tctccccttc aggggagaga tacgaagggt
    11041 tggcagcttg ctgcctagcc gcagtagaga gggggctgga ccatgtcccc ctctcccaac
    11101 cctctccccc tgaaggggag agggctatat gtccgctcac caccccaaag ccgacaaccc
    11161 accttaccgc ttcacatgct gcctatcgcc ccacaggatc gcgcggtgct cgtcggtgaa
    11221 gccggtgagg ccgccctcga tcgtttcggc gcgcgcgacg ccgaccgtca tgccctcggc
    11281 gaccgccgga cgttcgagca tcgcggcgcc ccagcgatcg acgttcggaa agcgccccgc
    11341 cttttcaatc aactgccggc gcaaataggg cagggtcgcc atgtcggcga tcgtatagtc
    11401 gtcgcccgcg agccattcgg cttcgccgag gcgcttgtcg agcaccatca tcagccggtc
    11461 gacctcgcgg ctgtaacgcg cgatcgcata gtcgtgccga tccttggcat aatgggtgaa
    11521 atgcgcctcc tgcccgaaca tcgggccgac ccccgacacc tggaacatca gccattgcca
    11581 gcaggtcgcg cgcgccacgg gatcggtggg caggaaacgc ccggtctttt cggcgaggta
    11641 gagcaggatc gcgccggttt cccaaagcac gaaccgttcg ccaccggggc cttcttcgtc
    11701 gacgatcacc ggcgtcttgt tgttcggatt gagcgcgaga aattcgggcg tcagctgatc
    11761 gcccgcgagg atgtcgatat attcgagccg ccagtcgagc tccatatcta gcagcgcgat
    11821 cgcgatcttg cgcgcattgg gggtcgggcc gccatagagc gtgatcatcg catcaagtct
    11881 cccgtccgag ccagcgtttg agcacctcgg cgccatcctg tccgacagtc agcggggcgg
    11941 ggcggcgcac gctgccgggc gtcgccgaca gcttcgggaa caccccctgc atcgtgactt
    12001 cgccgaactc ctcgtcggcg accgtcacca gcgcgtcgcg cgcggcgaaa tgcgggtcgg
    12061 cgagcatatc ctgggcgtcg aagacgcggc ccgcgggcac gccggcttcg atcatcttcg
    12121 cttcgagttc ggcgatcgtc agcgtcgcgg tccattcgcc gatcagcgcg tcgagttccg
    12181 cctgccgcgc cccgcgcgcg cggtgcgtcg cat
//
LOCUS       DATMSU010000122         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_68636, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000122 DATMSU010000000
VERSION     DATMSU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68636"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VN034_03315"
     CDS             complement(<1..751)
                     /locus_tag="VN034_03315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HWV59657.1"
                     /translation="MSADALLPLLKSTFGFDHFRGRQGEVVDRVMAGERTLAVMPTGA
                     GKSLTYQLPAVALSGCVVVVSPLIALMHDQLRGARAAGIRAASLTSVDADFADTRQAY
                     RDGELDLLYIAPERATGEGFRSLMAARTPVLFAIDEAHCVSEWGHDFRPDYRLLRPLL
                     DAFPSVPRLALTATADKHTREDILLQLGIPAEGLVLAGFDRPNIRYRVTPRVSPAKQL
                     ADFIAANPGPGIVYCPTRDGTEQMAAKLAAAT"
     gene            complement(843..2486)
                     /gene="ybaL"
                     /locus_tag="VN034_03320"
     CDS             complement(843..2486)
                     /gene="ybaL"
                     /locus_tag="VN034_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HWV59658.1"
                     /translation="MPHDTTLIGTIVAGLGVAFLMGALAHRLKISPIAGYLLAGILVG
                     PFTPGFVADTGLAMQLAEIGVILLMFGVGLHFSLKDLLSVRKIAVPGAIAQIAVATAL
                     GMMLGLWLGWSIEGSAVFGLALSVASTVVLLRALQARDMVETEKGRIAVGWLIVEDLV
                     MVLALVLLPAMFGDRGDEANGAGLLQSAAIVLIKVVGFGAFMFLIARRILPWVLHWVA
                     HSGSRELFRLAVLAIALGVAFGAAVIFDVSFALGAFFAGMILGETQLSRRAAEETLPL
                     RDAFAVLFFVSVGMLFNPEVLTEQPGPVVATVAIIVVGKSLAAYALVRAFGHPNQTAL
                     TVSVSLAQIGEFSFILAGLGVGLDVLPETGRDLILAGSMLSILFNPILFTLAVKRIRS
                     DEPAAEQPEGAAAETPEPCNAGVVLIGYGRVGSHIGGMICGRGEGLTVIEDQKDMAAA
                     AEAAGATVIVGDATKESILRQAGLDAASTLLIAIPEGVEAGAIVRRARAINPKLVIVA
                     RAHSDEEVNDLVRRGADHVVMAERETASRMAERAMLTRA"
     gene            complement(2556..4598)
                     /locus_tag="VN034_03325"
     CDS             complement(2556..4598)
                     /locus_tag="VN034_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWV59659.1"
                     /translation="MIRLAMCIAIDHDPWLVALAVAICGVGAFAIVQMFERARDASRA
                     QGIAWAFLVAVAASATIWCTHFVAMLAFEAKAPVTLDPVLTIASLMVAMVGSFIGFAV
                     AAWGKDEVFGAIGGALFGAAIAAMHFTGMTAYRVDGIVAWDGEYIAAAVICGMAFAAA
                     ALTVLRTRRAVRYRVALATLLIVLAIASLHFLAMTAMRITPMALDETPLRPAEFNALA
                     LATVLVGGIVIAAGVFAAMLDRQTRSEALRELMRMAMSDALTGLPNRTAFRAELARQI
                     GAAMVSGERVGLCAIDLDRFKEINDVHGHKAGDEVLARIAQRMRDALGHNEFVARLGG
                     DEFVAMTRFTDRAQLAAFAARLDAELKTPLIFPDFEARLGASIGVAVFPDDAATAEML
                     ANNADLAMYRAKADAATTPCHYDWQLDEAIRDQRELATDLRSAIDRDQLDVHYQVQTS
                     VTTGEITGYEALLRWTHPVRGAIPPAIFIPLAEANGFILTLGEWVMRRACTDAAAWPH
                     ASKVAVNVSPLQLAHVDLPRLFHQILLDTGLPPRRLEIELTETAIIADRERALHVLRQ
                     IKALGVGVALDDFGTGYSSLETLRAFPFDKIKLDRFFLAELAGNVQSTAILRAVLALG
                     KSLSIPILAEGIETAEQLDVLRREGCDEAQGFLIGRPGRTAGGVGDRGDSERVHAA"
     gene            complement(4715..6535)
                     /locus_tag="VN034_03330"
     CDS             complement(4715..6535)
                     /locus_tag="VN034_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HWV59660.1"
                     /translation="MPPDTSTSADASREPPVLATLRRFLPYLWPAGQTEAKVRIVLAG
                     LIVLASKGVQLSMGFLYGAAIDRMAPGMEEGVALAIGLVLAYAGARFAGVLFDNLRNV
                     VFERVGQDATRELAIGTFSHLHALSLRFHLARRTGEVTKVIERGTKSIDTMLYFLLFN
                     IAPTIVELAAVLIIFWTKFSFGLASATALMVVVYIIFTRKVTDWRNALRTRMNDLDTL
                     TIGRSVDSLLNYETVKYFGAEEREEARYRDVADQYARAAVKSENSLAWLNMGQSLITN
                     AALAGAMAYTVWGWSTGEYKVGDVVLVNTLLAQLFRPLDMLGMVYRVIRQGLIDMEAM
                     FRLIDTPPEIADAPDAWPLVVNGGAVAFENVVFGYEPDRTILNGVSFAVGAGETLAIV
                     GPSGAGKSTIARLLFRFYDPQGGRITIDDQDIAQVTQKSLRAEIGIVPQDTVLFNDSI
                     GYNIAYGREGANADEIAAAAEGAAIDSFIALLPQGYDTEVGERGLKLSGGEKQRVAIA
                     RTLLKNPPLLILDEATSALDSRTEAAIQSTLERIAARRTTLVIAHRLSTVTNADRIIV
                     LERGRVAETGTHDQLLGMRGLYADMWARQQAERDEGTVDA"
     gene            complement(6582..6731)
                     /locus_tag="VN034_03335"
     CDS             complement(6582..6731)
                     /locus_tag="VN034_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59661.1"
                     /translation="MRFSPIAPPRRPLFGRRQEPPAAIVPTPTSEERRLIDRIIRRAG
                     YRESR"
BASE COUNT         1199 a         2385 c         2315 g         1041 t
ORIGIN      
        1 ccgtcgccgc cgcgagcttc gccgccatct gttcggtccc gtcgcgcgtc gggcaataga
       61 cgatccccgg ccccgggttc gcggcgataa agtcggcgag ctgcttcgcc gggctcacgc
      121 gcggcgtgac ccgatagcga atgttcgggc ggtcgaaccc cgcgagcacc aacccttcgg
      181 ccggaatgcc gagctggagg aggatatcct cgcgcgtgtg cttgtccgcc gtcgccgtca
      241 gcgcgagccg cggcaccgag ggaaaggcgt cgagcaaagg ccggagcagc cggtaatcgg
      301 gccggaaatc atgcccccat tcgctgacgc aatgcgcctc gtcgatcgcg aacaatacgg
      361 gcgtgcgcgc cgccatcagc gaccggaaac cttcgccggt ggcccgctcg ggcgcgatat
      421 agagaagatc gagttcgccg tcgcgatacg cctgccgcgt gtcggcgaaa tcggcatcga
      481 cgctcgtcag actcgccgcg cgaatccccg ccgcgcgcgc gccgcgcagc tggtcgtgca
      541 tcagcgcgat cagcggcgac acgacgacga cgcagccgga gagcgccact gcgggcaatt
      601 gataggtcag cgacttgccc gccccggtcg gcatcaccgc cagcgtgcgc tcgcccgcca
      661 tcacgcgatc gacgacctcg ccctgacggc cgcggaaatg atcgaaaccg aaggtggatt
      721 tgagcagcgg gaggagcgcg tcggcggaca ttggaatggg cgatagggaa gatgaggtgg
      781 agagggaagc cccgacctcc tccaacatgc cgcagaacca acgaggatcg ggatagctga
      841 ggctatgccc gcgtcagcat cgcccgctcg gccatccgcg acgcggtctc gcgctcggcc
      901 atcacgacat gatcggcgcc gcggcgcacg aggtcgttca cttcctcgtc cgaatgcgcg
      961 cgcgcgacga tgaccagctt cgggttgatc gcgcgcgcgc gccgcacgat cgcgcccgcc
     1021 tcgacccctt cgggaatcgc gatcagcagc gtcgacgccg catcgagtcc cgcctggcgc
     1081 aggatgctct ccttcgtcgc gtcgccgacg atcaccgtcg cgcccgcggc ctcggcggcg
     1141 gcggccatgt ccttctggtc ctcgatcacc gtcagccctt cgccgcgtcc gcagatcatc
     1201 ccgccgatat ggctgccgac gcggccatag ccgatcagga cgaccccggc gttgcacggc
     1261 tcgggcgttt cggcggccgc gccctccggc tgctcggcgg cgggctcgtc ggaacggatg
     1321 cgtttgaccg ccagcgtgaa gaggatcggg ttgaacagga tcgacagcat cgaccccgcg
     1381 aggatcaggt cgcgcccggt ttcgggcagc acgtcgagcc cgacgcccag cccggcgagg
     1441 atgaacgaaa attcgccgat ctgcgcaagg ctgaccgata cggtcagcgc ggtctggttg
     1501 ggatggccga atgcgcggac tagcgcatag gcggcgagcg acttgccgac gacgatgatc
     1561 gccaccgtcg cgacgaccgg tcccggctgt tcggtcaaga cttcgggatt gaacaacatc
     1621 cccaccgaca cgaagaagag cacggcaaag gcatcgcgca gcggcagcgt ctcctcggcc
     1681 gcgcggcgcg acaattgcgt ctcgccgagg atcatgccgg cgaagaaggc gccgagcgcg
     1741 aaactcacgt cgaaaatcac cgccgcgccg aatgcgaccc ccaaggcgat cgcgagcacc
     1801 gcgagccgga acagctcgcg cgatcccgaa tgcgcgaccc agtggagcac ccaaggcagg
     1861 atccgccgcg cgatcaggaa catgaaggcg ccgaaaccga cgaccttgat cagcacgatc
     1921 gccgccgact ggagcaagcc ggcaccattc gcctcatcgc cgcgatcgcc gaacatcgcg
     1981 ggcagcagca cgagcgcgag caccatgacg agatcctcga cgatcagcca gccgaccgcg
     2041 atccgcccct tttcggtctc gaccatgtcg cgcgcctgca gcgcgcgcag cagcacgacg
     2101 gtcgacgcga ccgacagcgc cagcccgaac accgcgctcc cctcgatcga ccagccgagc
     2161 cacaggccga gcatcatgcc gagcgcggtc gcgaccgcga tctgcgcgat cgcccccggg
     2221 accgcgatct tgcggaccga cagcaggtcc ttgagcgaaa agtggaggcc gaccccgaac
     2281 atgaggagga tgacgccgat ctcggcgagc tgcatcgcca gccccgtgtc ggcgacgaag
     2341 cccggggtga agggcccgac gaggatgccc gccagcagat agcccgcgat cggcgagatt
     2401 ttgagccggt gcgcgagcgc ccccatcagg aaggcgaccc ccaggcccgc gacgatggtt
     2461 ccgatcagcg tggtatcatg cggcatgaac cctgcgtaaa gggcgcgtgc gcggggggtc
     2521 aacccaaggc aagtgattta gtttaaattg cgccgtcagg cggcgtgaac ccgctcgctg
     2581 tcgccccgat cgccgacccc tcccgccgtg cgccccgggc gtccgatcag gaagccctgc
     2641 gcctcgtcgc atccctcgcg gcgcagcacg tcgagctgct cggcggtctc gatcccctcg
     2701 gcgaggatcg ggatcgacag gcttttgccg agcgcgagca ccgcgcgcag gatcgcggtc
     2761 gactggacgt tgccggcaag ttcggcgagg aagaagcggt cgagcttgat cttgtcgaac
     2821 ggaaaggcgc gcagcgtttc gagcgacgaa tagccggtgc cgaaatcgtc gagcgcgacc
     2881 ccgacaccga gcgccttgat ctggcgcagc acgtgcagcg cgcgttcgcg gtcggcgatg
     2941 atcgcggtct cggtcagctc gatctccagc cgccgcggcg gcaggccggt gtcgagcaat
     3001 atctgatgga acaggcgcgg caggtcgaca tgcgcaagct gcagcggcga cacattgacc
     3061 gcgaccttcg aggcgtgcgg ccaggcggcc gcgtcggtgc acgcgcgccg catcacccat
     3121 tcgccgagcg tcaggatgaa accgttcgct tcggcgagcg ggatgaagat ggcgggcggg
     3181 atcgctccgc gaaccggatg cgtccagcgc agcagcgctt catatccggt gatctcgccg
     3241 gtcgtgaccg aggtctggac ctgataatgg acgtcgagct ggtcgcgatc gatcgcgctg
     3301 cgcaggtcgg tcgcgagttc gcgctggtcg cggatcgcct cgtcgagctg ccagtcataa
     3361 tggcacggcg tggtcgcggc gtcggccttc gcgcggtaca tcgcgaggtc ggcgttgttc
     3421 gccagcatct cggcggtcgc cgcatcgtcg ggaaagaccg cgacgccgat gctcgcgccg
     3481 agccgcgcct cgaaatcggg aaagataagc ggtgttttga gctcggcatc gagccgcgcg
     3541 gcaaaggccg cgagctgcgc gcggtcggta aagcgcgtca ttgcgacgaa ttcgtcgccg
     3601 cccaatcgcg cgacgaactc gttatgcccc agcgcatcgc gcatccgctg cgcgatgcgc
     3661 gccagcacct cgtcgcccgc cttgtggcca tggacgtcgt tgatttcctt gaagcggtcg
     3721 agatcgatcg cgcagaggcc gacgcgctcg ccgctcacca tcgccgcgcc gatctgccgc
     3781 gccagttcgg cgcgaaacgc ggtgcgattg ggcaggcccg tcaacgcgtc gctcatcgcc
     3841 atccgcatca gttcgcgcag cgcctccgac cgcgtctggc ggtcgagcat cgcggcgaag
     3901 acgcccgccg cgatcacgat cccgccgacg aggaccgtcg cgagcgcgag cgcgttgaac
     3961 tccgccgggc gcagcggcgt ctcgtcgagc gccatcgggg tgatccgcat ggcggtcatc
     4021 gcgagaaaat ggagcgaagc gatcgcaagg acgatgagca aagtcgcgag cgcaacgcgg
     4081 tatcgcaccg cccggcgcgt gcgcagcacg gtcagcgcgg cggcggcgaa ggccatgccg
     4141 cagatcaccg ccgcggcgat atattcgccg tcccacgcga cgatgccatc tacacggtac
     4201 gcggtcatgc cggtgaagtg catcgcggcg atcgcggcgc cgaacagcgc gccgccgatc
     4261 gcgccgaaaa cctcgtcctt accccaggcc gcgacggcga atccgatgaa gctgccgacc
     4321 atcgcgacca tcagcgatgc gatggtcagc accgggtcga gcgtcaccgg cgccttggct
     4381 tcgaaggcga gcatcgcgac gaaatgggtg caccagatcg tcgcgctcgc ggccacggcg
     4441 acgaggaagg cccaggcgat tccctgcgcg cgcgacgcat cgcgcgcgcg ctcgaacatc
     4501 tggacgatcg caaaggcgcc gacgccgcag atcgcgaccg ccagtgcgac gagccacgga
     4561 tcgtggtcga tcgcgataca catcgccaac ctgatcaaga tcccaccttc ccctcgaacg
     4621 cccgccggcc tgacggacgg gtccaaagag gcccaatggg tgggaatcac ttacaaaaat
     4681 gtaaatccaa gtaaaaatag tgcgaccgcc gcgatcaggc gtcgaccgtc ccctcgtcgc
     4741 gctccgcctg ctgccgtgcc cacatgtcgg cgtagaggcc gcgcatgccc agcaactggt
     4801 cgtgcgttcc cgtctcggcg acccgtccgc gttcgagcac gatgatgcgg tcggcgttcg
     4861 tcaccgtcga caggcggtgc gcgatcacca gcgtcgtgcg ccgcgcggcg atacgctcaa
     4921 gcgtcgactg gatcgccgcc tcggtgcggc tgtcgagcgc gctcgtcgct tcgtcgagga
     4981 tcagcagcgg cggattcttg agcagcgtgc gcgcgatcgc gacccgctgc ttctcaccgc
     5041 ccgacagttt cagcccgcgc tcgccgacct cggtgtcgta accttgcggc agcaacgcga
     5101 tgaagctgtc gatcgcggca ccttcggccg ccgccgcgat ttcgtcggcg ttcgcgcctt
     5161 cgcggccgta ggcgatgttg taaccgatcg aatcgttgaa cagtaccgta tcctgcggaa
     5221 cgatgccgat ttcggcgcgc aggctttttt gcgtcacctg cgcgatgtct tgatcgtcga
     5281 tcgtgatccg tccgccttgg ggatcataga agcggaagag cagccgcgcg atcgtcgact
     5341 tgcccgcgcc cgacggcccg acgatcgcca gcgtctcgcc cgcgccgacc gcgaagctga
     5401 cgccgttcag gatggtgcgg tcgggctcat agccgaacac gacattctcg aacgccaccg
     5461 cgccgccgtt gacgaccagc ggccacgcat cgggcgcgtc ggcgatctcg ggcggggtgt
     5521 cgatcaggcg gaacatcgcc tccatgtcga tcaacccttg ccggatcacg cgatagacca
     5581 tgccgagcat gtcgagcggg cggaagagct gcgcgagcaa agtgttcacc agcaccacgt
     5641 cgccgacctt atattcgccg gtcgaccagc cccacaccgt ataggccatc gcccccgcga
     5701 gcgcggcgtt ggtgatcagg ctctgcccca tgttgagcca ggcgaggctg ttctcgctct
     5761 tcaccgcggc gcgcgcatat tgatcggcga cgtcgcggta gcgcgcttcc tcgcgctcct
     5821 cggcgccgaa atatttgacc gtttcgtaat tgagcaggct gtcgacgctg cgcccgatcg
     5881 tcagcgtgtc gaggtcgttc atccgcgttc tgagcgcgtt gcgccagtcg gtcaccttgc
     5941 gcgtgaagat gatgtagacg acgaccatca gcgcggtcgc gctcgcgagg ccgaagctga
     6001 atttggtcca gaagatgatc agcaccgccg cgagttcgac gatcgtcggc gcgatgttga
     6061 agagcaggaa atagagcatc gtgtcgatgc tcttggtgcc gcgctcgatc accttggtga
     6121 cctcgcccgt gcgccgcgcg aggtggaagc gcaggctcag cgcgtggagg tggctgaagg
     6181 tgccgatcgc gagttcgcgc gtcgcatcct gcccgacgcg ttcgaacacg acgttgcgga
     6241 ggttgtcgaa aagcaccccg gcaaagcgcg cgccggcata agcgagcacc agcccgatcg
     6301 cgagcgcgac gccttcctcc atacccggcg ccatgcggtc gatcgcggcg ccatagagga
     6361 agcccatcga cagctgcaca cccttcgacg cgagcacgat gagcccggcc aggacgatgc
     6421 ggaccttcgc ctcggtctgt cccgccggcc acagataagg cagaaaacgc cgcagcgtcg
     6481 caaggacggg cggttcgcgg ctggcgtcgg cggaggtgga ggtatcgggg ggcatggacg
     6541 ccccatgtag gggcgtaacg ccgccgcgcc aactccctcg tctagcgcga ttcgcgatag
     6601 cccgcgcggc ggatgatgcg atcgatcagg cgccgctctt cggatgtcgg cgtcggcacg
     6661 atcgccgcgg gcggttcctg gcggcggccg aacagcggac gccggggcgg ggcgatgggg
     6721 gaaaagcgca tgggggctgc tttcaactgc gcggcgtcgc cccccggcac cgctatgacc
     6781 tggtcccgcg gccatggtga agccgcatgg ttgcggcccc gtaaagcgcc ttggttactg
     6841 ctttctttcc ggtaccgtga aggtgccggt tacgcgccca ccggtgccgc cctgcaccgg
     6901 attgccgtcg ggcccgcgcg ccgcgccgcg cccgtcgacc
//
LOCUS       DATMSU010000123         3860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_125741,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000123 DATMSU010000000
VERSION     DATMSU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125741"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VN034_03340"
     CDS             complement(<1..140)
                     /locus_tag="VN034_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV59662.1"
                     /translation="MAAGVPAAAQDVAITNAKLVIGDGSAPIEGGTVVVRGGKVVAAG
                     AGV"
     gene            complement(272..1675)
                     /locus_tag="VN034_03345"
     CDS             complement(272..1675)
                     /locus_tag="VN034_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV59663.1"
                     /translation="MIRGVKASLLAAVSLALVGCAATGKETASASDKPAEKPAKFNKD
                     PYPSTYKPYPGQVTAVRNVTIFDGEGGRIENGTVLLAEGKVEAIGGPETPIPADIAVF
                     DGTGKFLTPGVIDIHSHLGDYPSPSVDAHSDGNEATAPTTPEVWSEHSVWPQDPGFSR
                     ALANGGVTSLQILPGSANLMGGRSITLKNVPSRTVQGMKFPGAPYGLKMACGENPKRV
                     YGGKGRMPSTRMGNFAVNRATWAKAAAYKKKMDDGKAVDRDLAMETLAGVLAGEILVH
                     NHCYRADEMALVIDMSKEFGYKVSTFHHAVEAYKIADILAKEGICSAMWADWWGFKME
                     AYDSVNENIPLVYKAGACTIVHSDDANQIQRLNQEAAKARAAGRRIGIDVSDEMAWTW
                     LSYNPAKALGIADRTGSLKPGKMADVVLWNGNPFSAYTRPEKVWIDGALMFDAMNPER
                     RPVSDFELGQPGEGDVK"
     gene            complement(1679..3514)
                     /locus_tag="VN034_03350"
     CDS             complement(1679..3514)
                     /locus_tag="VN034_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HWV59664.1"
                     /translation="MKDMGLWDEGDWIAVIALVVLGVFLSIGALIAGSKKPEFAGHPK
                     GLYMLFFAEMWERFSYYGMRAILIFYLTQHWLFNDSKSNLIYGAYTSLVYITPVLGGY
                     LADRYLGQRKAVLFGGLLLAAGHSLMAVEGVGGQNDPTINVFWAALAFIIVGSGFLKA
                     NISVMVGQLYRLTDIRRDSAYTIFYMGINVGAALGTILVGYLGQRIGWGYGFGLAGIG
                     MLAGLVVFVLGKSALKGAGEAPAPLPRAREWTLYAVGLAAVAVIWALVQYQDVIQNLL
                     LVSGILLLGYVVLVATFQLPYGSIGAGPRSRVNLALAGLVLMLFLPLALILGLGIASG
                     PVALGLAPAAWVALFGFLLLLGVMVVEKMNREDHARDRMFAILFLIALNPLFWGLFEQ
                     AGGSMNLFTDRYVDRGAVPASIFQSINPIYIILLAPLFAALWQWLGKRGKEPSAPAKF
                     GLALAQMGLANLILVWGAQAFGIAAMTPVIFVFLYYLLATTGELCLSPVGLSAMNRLA
                     PTFVASLIMGAWFYMTAVGNFVAGKIGEATGGHGGEMSKAKLLEIYEMFGWIAIGAAV
                     AVLLVSPIVKRWMHLDTLRDRDDGLAGVGEAEPQAGGVHPEPKGA"
     gene            3701..>3860
                     /locus_tag="VN034_03355"
     CDS             3701..>3860
                     /locus_tag="VN034_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HWV59665.1"
                     /translation="MFNDTSSLLAHLATRRSGKARDMVAPGPDAATLHDIVALALRTP
                     DHGKLAPWR"
BASE COUNT          709 a         1278 c         1220 g          653 t
ORIGIN      
        1 acgcccgcac ccgccgcgac gaccttgccg ccgcgcacga cgacggtgcc gccctcgatc
       61 ggcgcgctgc cgtcgccgat gacgagcttg gcattggtga tggcgacatc ctgcgccgcc
      121 gcgggaactc cagcggccat caacgcagtc aaagcgactc ccaaccccgc tcgtgtcgag
      181 cgaagtcgag acaccccaaa ggagcgcacg accgatgggc atctcgactt cgctcgatgc
      241 gaacgggaaa ggaaggctgt gaaggatttt ttcatttcac atctccttcg cccggctggc
      301 cgagttcgaa atcgctcacc ggccgccgct cggggttcat cgcgtcgaac atcagcgcgc
      361 cgtcgatcca cactttttcg gggcgcgtat aggcgctgaa cggattgccg ttccacagca
      421 cgacgtcggc catcttgccg ggcttcaggc tgccggtccg gtcggcgatg cccagcgcct
      481 tggccgggtt atacgacagc caggtccacg ccatctcgtc gctgacgtcg atgccgatgc
      541 ggcgccccgc ggcgcgcgcc ttggcggctt cctggttcag ccgctggatc tggttcgcgt
      601 cgtccgaatg gacgatcgtg caggcgccgg ccttgtagac gagcggaata ttctcgttga
      661 ccgagtcata ggcttccatc ttgaagcccc accagtcggc ccacatcgct gaacagatgc
      721 cttccttcgc aaggatgtcg gcgatcttgt acgcctcgac cgcatggtga aaggtcgata
      781 ccttgtagcc gaattccttc gacatatcga tgacgagcgc catttcgtcg gcgcggtagc
      841 aatgattgtg gacgaggatt tcgcccgcca gcacgcccgc gagcgtttcc atcgcgaggt
      901 cgcggtcgac cgccttgccg tcgtccatct tcttcttgta cgcggccgcc ttcgcccagg
      961 tcgcgcggtt caccgcgaaa ttgcccatgc gggtcgaggg catccgcccc ttgccgccat
     1021 agacgcgctt cggattctcg ccgcacgcca ttttgaggcc atagggcgcg ccgggaaact
     1081 tcatcccctg caccgtgcgc gacggcacat tcttgagcgt gatcgagcgc ccgcccatca
     1141 ggttcgcgct gcccggcagg atctgcaggc tggtgacgcc gccgttcgcg agcgcgcggc
     1201 tgaacccggg atcctgcggc cagacgctat gttcggacca gacttcgggg gtcgtgggcg
     1261 cggtcgcctc gttgccgtcc gaatgcgcat cgaccgatgg cgagggatag tcgccgaggt
     1321 ggctgtggat gtcgatgacc ccgggggtga ggaatttgcc ggtgccgtca aagaccgcga
     1381 tgtccgcggg gatcggcgtt tcggggccgc cgatcgcctc gaccttgccc tctgccagca
     1441 gcacggtgcc attttcgatc cggccgccct cgccatcgaa gatcgtgacg ttgcgcaccg
     1501 cggtgacctg ccccggatag ggcttgtagg tcgagggata aggatccttg ttgaacttcg
     1561 ccggcttttc ggccggcttg tcgctcgccg atgccgtttc cttgccggtc gccgcgcaac
     1621 cgacgagcgc gagcgacacc gcagccagca gactggcttt cacaccacgg atcatggatt
     1681 acgccccctt gggttcggga tgaacgccgc ccgcctgcgg ttcggcttcg ccgaccccgg
     1741 ccagaccgtc atcgcggtcg cgcagcgtgt cgaggtgcat ccagcgcttg acgatcgggc
     1801 tgaccagcag caccgccacc gccgcaccga tcgcgatcca gccgaacatc tcgtagattt
     1861 ctagcagctt ggccttgctc atctcgccgc catgcccgcc ggtcgcttcg ccgatcttgc
     1921 cagcgacgaa attgccgacc gcggtcatat agaaccacgc gcccatgatg agcgacgcga
     1981 cgaaggtcgg cgccaaccgg ttcatcgccg agaggccgac ggggctgagg cacagctcgc
     2041 cggtggtggc gaggaggtaa taaaggaaga cgaagatgac cggggtcatc gccgcgatgc
     2101 cgaatgcctg cgcgccccac acgaggatga ggttggcaag ccccatctgc gccagcgcca
     2161 gcccgaattt ggccggggcc gacggttcct tgccgcgctt gccgagccat tgccacagcg
     2221 cggcgaacag cggggcgagc aggatgatgt agatcgggtt gatcgactgg aagatcgaag
     2281 cgggcaccgc gccgcgatcg acgtagcggt cggtgaacag gttcatcgac ccgcccgcct
     2341 gttcgaacag gccccagaac agcgggttca gggcgatcag gaacaggatc gcgaacatcc
     2401 ggtcgcgcgc atggtcttcg cggttcatct tctcgaccac catcacgccg aggaggagga
     2461 ggaagccgaa cagcgccacc catgccgcgg gcgcgagccc gagtgcgacg ggtccgctgg
     2521 cgatgccgag gccaaggatg agcgccagcg gcaagaacag catgaggacg aggcccgcga
     2581 gcgcgaggtt gacgcggctc cgcgggccgg cgccgatcga tccgtagggc aactggaacg
     2641 tcgccaccag gacgacgtag ccgagcagca ggatgcccga aacgagcagg aggttctgga
     2701 tgacgtcctg atactggact agagcccaga tcaccgcgac cgcggcgagg ccgacggcat
     2761 aaagcgtcca ttcgcgtgcc cgcggcagcg gcgcaggcgc ttcgcccgcg cccttgagcg
     2821 cgctcttgcc gagaacgaaa acgacgagac cggcgagcat gccgatgccg gcgagcccga
     2881 agccgtagcc ccagccgatc cgctgaccca gatagccgac gaggatcgtg ccgagcgcgg
     2941 cgccgacgtt gatgcccata tagaagatcg tataggcgct atcgcggcga atgtcggtga
     3001 gccggtacaa ttgccccacc atcaccgaga tattcgcctt caaaaagccc gagccgacga
     3061 tgatgaaggc aagcgctgcc cagaagacgt tgatcgtcgg gtcattctgc ccgccgacac
     3121 cttcgaccgc catcaggctg tggccggcgg ccagcagcag gccgccaaac agcaccgcct
     3181 tgcgctgccc gagataccgg tcggcgagat aaccgccgag gaccggggtg atgtagacga
     3241 ggctggtata ggcgccgtag atcaggttcg acttgctgtc gttgaacagc cagtgctgcg
     3301 tcaggtagaa aatcaggatg gcgcgcatgc cgtagtagga gaaacgctcc cacatctcgg
     3361 caaagaagag catatacagg cccttcggat gtccggcgaa ttcgggtttc ttgctgccgg
     3421 cgatcagtgc gccgatgctc aggaagacgc cgaggacgac gagcgcgatc accgcgatcc
     3481 agtcgccttc gtcccaaagg cccatatctt tcatcaaagc gtcccttttt atcgattatc
     3541 ttgagcacgg cctcccgccg cgcgaatggg gcaacctagc gcgaaaattg cgtatgggaa
     3601 gaatctaatt gcgcctgaaa ccggttgggt cgggccaaat tttgttgcga atcactcgca
     3661 acacttgcgc gagggcgcgg aaaagcccac atgccgcagc atgttcaacg acacctcctc
     3721 gctcctcgct catctcgcca cccgccgctc gggcaaggca cgcgacatgg tcgcgcccgg
     3781 ccccgacgcc gcgacattgc acgacatcgt cgcgctcgcg ctgcgcaccc ccgaccatgg
     3841 caagctcgcg ccctggcgca
//
LOCUS       DATMSU010000124         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_87748, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000124 DATMSU010000000
VERSION     DATMSU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87748"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VN034_03360"
     CDS             complement(<1..633)
                     /locus_tag="VN034_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWV59666.1"
                     /translation="MELRPLHIMGAQLVGADAPRPGSLLAVDGRIAAIDPTELPEGTE
                     TVDAKGQWLAPGIIDLGVFATDKPAFHFGGITRAALMPDQSSTLDEAGLIRQAARAGK
                     PDFWVHPIAAATRGLKGTELAEIGMMQAAGAKAVATGRQWIADSGVMMRVLAYASGLG
                     LTVISHAEDSGLAGKAVATSGETATRLGLSHAPACAEAIAIARDIMLARET"
     gene            complement(624..1619)
                     /locus_tag="VN034_03365"
     CDS             complement(624..1619)
                     /locus_tag="VN034_03365"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWV59667.1"
                     /translation="MTNSTTRPASDYPPGGDAFRHRHLTGIAPLTPWEISYVLDAAEE
                     WVELNRSGAAKHDDRLAGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVK
                     KGETLIDTAMTLNAMRADAIVIRHASSGAVQLIADKVDCPVLNAGDGRHEHPTQALLD
                     ALTIRRRLGRVEGLTIAICGDVLHSRVARSNILALTLLGNEVRVVAPPTLTPPAMERM
                     HVTTFTDMDEGIKDADVVMMLRLQNERMDGAYLPSAREYHALYGLTPKRLEQAKPDAI
                     VMHPGPMNRGVEIDSSIADDPARSTITEQVEMGVAVRMACLDILTRRKRGVPGWN"
     gene            complement(1800..2258)
                     /gene="ruvX"
                     /locus_tag="VN034_03370"
     CDS             complement(1800..2258)
                     /gene="ruvX"
                     /locus_tag="VN034_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWV59668.1"
                     /translation="MITTAAPEFAANFPSGGRLAGLDVGTKTIGVAFCDTNWSFATPD
                     KTILRKKFAVDLETLKALVTQHNIVGLVVGLPLNMDGSDSPRTQSTRAFARNLAPLGL
                     PLLLWDERWSTAAVERAMIAADVSRAKRAERVDSAAAAFILQGAIDAMTR"
     gene            complement(2432..>3511)
                     /locus_tag="VN034_03375"
     CDS             complement(2432..>3511)
                     /locus_tag="VN034_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HWV59669.1"
                     /translation="DGSETPGTQLPGEKAAGQLIPPAKAAQAAAEAPFPRGNAAVFFV
                     HPTSYYSRSSWNAPLEDRDSDHRANLFVQGMASAFADAGEIWAPRYRQATLGAFLAQD
                     RVTAGKAIDSAYRDVEEAFDAFLDAQPKDKPIILAGHSQGALHLTTLLRTKIAGTPLA
                     KRIVAAYIIGWPVSLETDIAGLGLPACQTPEQKGCILSWASFADPADPVMVTDAYDGT
                     IGFDGRPRADTRMLCTNPLTGIPDTAAPPEANIGTLKPTEGFKSGSLIAGKIGATCDD
                     TRGFLMIGDADIAKEYVVAGYVLPGNNYHVYDITLFWANVRADALRRLATYEGKPSPV
                     PPPAEPLAPEAPTPAPASPSPSPRT"
BASE COUNT          517 a         1041 c         1331 g          622 t
ORIGIN      
        1 ggtttcacgc gccagcatga tgtcgcgggc gatggcgatg gcttcggcgc aggctggcgc
       61 atgtgacagg ccgagacgtg tcgcggtttc gccgctggtc gccaccgcct tgccagcaag
      121 gccgctgtct tcggcgtggg aaatgacggt gaggccaagg cccgaggcat aggcgaggac
      181 gcgcatcatg acgccggaat cggcaatcca ctggcgaccg gtggcgaccg ccttcgcgcc
      241 cgcagcctgc atcatgccga tttctgcgag ctctgtaccc ttcaggcccc ttgtggcggc
      301 ggcgatgggg tgtacccaga agtccggttt cccagcacga gcggcctggc ggatgagccc
      361 ggcttcgtcg agcgtggagg attggtcggg catgagcgcc gcccgcgtga tgccgccgaa
      421 atggaaggcg ggcttgtcgg tcgcgaagac gccgaggtcg atgatgcccg gagcgagcca
      481 ctggcccttg gcgtcgacgg tttcggtgcc ttcgggaagc tcggtgggat cgatggcggc
      541 gatacgccca tcgaccgcga gcagactgcc cgggcgcggc gcgtcggcgc ccaccagctg
      601 ggcacccatg atatggagcg gcctcagttc catcccggca ccccccgctt gcggcgcgtc
      661 aatatgtcga ggcaggccat gcgcaccgcg acgcccattt cgacctgttc ggtgatcgtc
      721 gagcgcgcgg ggtcgtcggc gatgctgctg tcgatctcga caccgcggtt catcgggccg
      781 gggtgcatga cgatggcgtc gggcttggcc tgttcgaggc gcttcggcgt caggccgtag
      841 agcgcgtgat attcgcgcgc cgagggcaga taggcgccgt ccatgcgctc gttctggagg
      901 cggagcatca tcacgacgtc ggcgtccttg atgccttcgt ccatgtcggt gaaggtggtg
      961 acgtgcatgc gctccatcgc gggcggggtg agcgtcggcg gcgcgacgac gcgcacctcg
     1021 ttgccgagca gggtgagcgc gaggatgttc gagcgcgcga cgcggctgtg gaggacgtct
     1081 ccacatatgg cgatggtgag accctcgacg cggccgagac ggcggcggat ggtgagcgcg
     1141 tcgagcaagg cctgcgtcgg atgctcgtgg cggccgtcgc cggcgttgag cacggggcag
     1201 tcgaccttgt cggcgatgag ctgcaccgcg cccgatgagg cgtgacggat gacgatcgcg
     1261 tcggcgcgca tcgcgttcaa tgtcatcgcg gtgtcgatca gcgtctcgcc ctttttcacg
     1321 ctcgactgcg cggcgtgcat gttgacgacg tcggcgccga ggcgcttgcc tgcgatctcg
     1381 aacgagagga gcgtgcgggt ggagttttcg aagaaggcgt tgatgatggt aaggcccgcg
     1441 agccggtcgt catgcttggc ggcgccgctg cggttcagtt cgacccattc ttcggcggcg
     1501 tcgaggacgt aggaaatctc ccacggagtg agcggagcga tgccggtgag gtggcgatgg
     1561 cgaaaggcat cgccgccggg cggatagtcg ctggcgggtc gggttgtgga gtttgtcatt
     1621 aaaggggagc gtctacgcag gcggcgagga aatcgcaagt ccgcgccaaa cttttccgca
     1681 tccccgagcg aagacgaggg gctatgccga gcgaagtcga ggcagattgg ctgcgcagtt
     1741 ctcgcttcgc tcgaacgagc ccctcgtctt cgctcgggga tgcggttttc ttttgcctgt
     1801 cagcgcgtca tcgcgtcgat cgcgccttgc aggatgaacg ccgcggcggc gctatcgacc
     1861 cgctccgccc ttttggcgcg gctgacatcg gcggcgatca tcgcgcgctc gaccgcggcg
     1921 gtcgaccagc gttcgtccca gagcagaagc ggcaggccga gcggggcgag gttgcgcgcg
     1981 aaggcgcggg tgctttgcgt gcgcgggctg tcgctgccat ccatgttgag ggggaggccg
     2041 acgacaaggc cgacgatatt gtgctgggtg acgagggcct tgagcgtttc gaggtcgacc
     2101 gcgaatttct tgcggaggat cgtcttgtcg ggggtcgcga agctccagtt cgtgtcgcag
     2161 aaggcgacgc cgatcgtttt cgtgccgacg tcgagccccg cgaggcggcc accgctgggg
     2221 aaattggcgg cgaattcggg ggcggcggtg gtgatcatcg ggctcttcct agaacatcat
     2281 cgcctttaag tgaaacatgt cacccctctt ccgtcatccc ggtgaaggcc gggatctcgc
     2341 cggtgcgtca ggccgtgagg gtgagatccc ggccttcgcc gggatgacga ttaaaataag
     2401 gaagcgggcg ggcggaaagc gggaccccgg atcaagtccg gggtgacggg gaaggggagg
     2461 caggagcagg tgtcggagcc tccggcgcca gcggttccgc tggcggcggc accggcgacg
     2521 gcttgccttc gtaggtcgcg agacggcgga gggcgtcggc gcggacgttg gcccagaaga
     2581 gggtgatgtc gtagacgtga taattgttgc cggggagcac atagcccgcg acgacatatt
     2641 ccttcgcgat gtcggcgtcg ccgatcatca ggaagccccg cgtgtcgtcg catgtcgcgc
     2701 cgatcttgcc cgcgatcagc gatcccgact tgaagccctc ggtgggcttg agcgtgccga
     2761 tattggcctc gggcggcgcg gcggtgtcgg ggatgccggt gagcgggttg gtgcagagca
     2821 tccgcgtatc ggcgcgcggg cggccgtcga agccgatcgt gccgtcatag gcgtcggtga
     2881 ccatcaccgg atcggcgggg tcggcgaagc tcgcccagct gaggatgcag cccttttgct
     2941 cgggcgtctg gcaggcaggc aggccgaggc cggcgatgtc ggtttcgagg ctgaccggcc
     3001 agccgatgat gtaggccgcg acgatccgct tcgcgagcgg cgtgcccgcg atcttcgtgc
     3061 ggagcaaagt ggtgaggtgg agcgcgccct ggctgtggcc cgcgaggatg atcggcttgt
     3121 ccttcggctg cgcgtcgagg aaggcgtcga acgcttcctc gacgtcgcga taggccgaat
     3181 cgatcgcctt gcccgcggtg acgcggtcct gcgcgaggaa ggcgccgagc gtcgcctggc
     3241 gatagcgcgg cgcccagatt tcgcccgcgt cggcaaaggc gctcgccatg ccctgcacga
     3301 agaggttggc gcgatggtcc gaatcgcggt cctcgagcgg ggcgttccag ctcgagcggc
     3361 tgtaatagct ggtcgggtgg acgaagaaga ccgcggcgtt gccccggggg aagggggctt
     3421 cggcggctgc ctgcgccgcc ttggcgggcg ggatcagctg gccggccgcc ttttcgcccg
     3481 gaagctgggt gccgggggtc tcgctaccgt c
//
LOCUS       DATMSU010000125         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_68652, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000125 DATMSU010000000
VERSION     DATMSU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68652"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2905
                     /locus_tag="VN034_03380"
     CDS             <1..2905
                     /locus_tag="VN034_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59670.1"
                     /translation="LNSYLGYTMWDYESDAPQMWPGPDNTAPRPPGMSDEEYQKKYKQ
                     QRYPTAAEVHQVLERNPEAAAARGLWADVEFLRDVYDVNDSNKDTQFADYHGHGWNFR
                     GIFKRDRNGNLMTADGNMATYGTDTANIIDPKDPEKWRKSCSHYTDPKERDLCLSSAD
                     PGRPGIEGKFVPPGLNPGKAVHMMDIHAEKGMQCADCHFAQDSHGNGYIQGEVANAVE
                     ISCKDCHGTADAYPNLLTSNVAARPQGTNLALLRNPDGRRRFEFQYNDDAEVIGLIQR
                     SIVDPKLEWQVSLVKQAVTPGPHFNAKAARAKLMARSGSEDGKFEWGPGVAKEDRAHG
                     DDRMTCFTCHLSWTTSCGGCHLPIEANWKTKSHHFEGEETRNFATYNPQVARDDMFQL
                     GRHQLTKPGEPGPNGEVRGIITPVRSTSALVLSSTNVNRERIYVQQPPISSAGYSSQA
                     FAPHFPHTVRRSETKQCSDCHLSAADDNNAIMSQLLLLGTNFVNFVGLNVWSGMEDGF
                     QATRVTEWDEPQAVIGSYLQKYAYPDYWKLHVDQNGRELKNWVRGKILDKKLSGETHA
                     LEEFANIVQDTSGRVNCLQQRGEYMYVAEGKGGFRVYDVASIGNKGFSERIVRAPFSP
                     MGHDTHVKTKNATCMAIATTQPISVSRNETIVKNFPENHEQVMHDIYRYAVITDSVEG
                     LVLVDIDTMADGEFRNNKLKRALTWNEGNVLAGARHITLAGSIAYVTTDAGLVVLDLT
                     IPLQPKVTAQLPLTDARASAVQFRYLWVTDAEGVKLFDVTDLAQPVAVPAGTVRLANA
                     RKIYLARTYMYVAAKADGLVIVDVTRPAAPIVWPGLTFGGAMTDAEDVIVASTNASLF
                     GYVADGRGGIKVLQLTSPDNPGLYGFSPKPTPELIAWAKTPSPALALSKGLDRDRAVD
                     ETGGQMAVFGRLGSRPFTRGEMEKLFLNSKGAVYKVRDEVDQSAWRPPLLYAN"
     gene            complement(3011..3718)
                     /locus_tag="VN034_03385"
     CDS             complement(3011..3718)
                     /locus_tag="VN034_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV59671.1"
                     /translation="MRVLLIEDEPTTAKAIDTMLTTEGFNVYTTDLGEEGLDLGKLYD
                     YDIILLDLNLPDMHGYDVLKKLRTAKVQTPVLILSGISEMDSKVRSFGFGADDYVTKP
                     FHRDELVARIHAVVRRSKGHSQSVIKTGKLAVNLDTKTVEVDTTRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLALACGGENYIETVWGRG
                     YVLRDIDDEGNEVRRVA"
     gene            complement(3910..>4527)
                     /locus_tag="VN034_03390"
     CDS             complement(3910..>4527)
                     /locus_tag="VN034_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HWV59672.1"
                     /translation="KVLGPPLAAFLDRHPAVEIEVLLSDARNDPVAEGIDLTLRIAPL
                     ADSSLLARTIAPVAASVIASPAYLEKHGVPKHPLDLADHRLIGYGHRQRAMPLHFHRK
                     GEEATVLPTGPLFANNGDIAVPLVVAGVGIALLPDFIAADALASGAVVPILTDWSPPQ
                     SHLHLLSPPSRLRPARVRALSDHLVETLKISCTGAHDQYLALHKG"
BASE COUNT          876 a         1459 c         1449 g          743 t
ORIGIN      
        1 cctgaactcc tacctcggct atacgatgtg ggactatgaa tcggacgcgc cgcagatgtg
       61 gccgggcccg gacaacacgg cgccgcgccc gccggggatg agcgacgaag agtatcagaa
      121 gaaatacaag cagcagcgct atccgaccgc cgccgaggtg caccaggtgc tcgaacgcaa
      181 ccccgaggcc gcggcggcgc gcgggctgtg ggccgacgtc gagttcctgc gcgacgtcta
      241 tgacgtcaac gacagcaaca aggacacgca gttcgccgac tatcacggcc atggctggaa
      301 cttccgcggc atcttcaagc gcgaccgcaa tggcaatctg atgaccgccg atggcaatat
      361 ggcgacctat ggcaccgaca ccgcgaacat catcgatccg aaggacccgg aaaaatggcg
      421 caagagctgc agccattaca ccgatccgaa ggagcgcgac ctgtgcctgt cgagcgccga
      481 tccgggacgt ccgggcatcg agggcaagtt cgtgccgccg gggctgaacc ccggcaaggc
      541 ggtccatatg atggacatcc acgccgaaaa gggcatgcag tgcgccgact gccacttcgc
      601 gcaggacagc cacggcaacg gctatattca gggcgaggtc gcgaacgcgg tcgagatcag
      661 ctgcaaggat tgccacggca ccgccgacgc ctatccgaac ctcctgacct cgaacgtcgc
      721 ggcgcggccg cagggcacca atctcgcgct gctccgcaac cccgacgggc ggcggcgctt
      781 cgaattccag tataatgacg atgccgaggt gatcgggctg atccagcgtt cgatcgtcga
      841 tccgaagctc gaatggcagg tcagcctggt caagcaggcg gtcaccccgg ggccgcattt
      901 caacgccaag gcggcgcgcg ccaagctgat ggcgcggtcg ggcagcgagg acggcaagtt
      961 cgaatggggt cccggcgtcg cgaaggaaga ccgcgcgcac ggcgacgaca ggatgacctg
     1021 cttcacctgt cacctctcat ggaccaccag ctgcggcggc tgccatttgc cgatcgaggc
     1081 gaactggaag accaagtcgc accatttcga gggcgaggag acgcgcaact ttgcgaccta
     1141 caacccgcag gtcgcgcgcg atgacatgtt ccagctcggc cggcaccagc tcaccaagcc
     1201 cggcgagccc ggcccgaacg gcgaggtgcg cggcatcatc accccggtgc gctcgacctc
     1261 ggcgctggtc ctgtcgtcga cgaacgtcaa ccgcgaacgc atctatgtgc agcagccgcc
     1321 gatctcctcg gcgggctatt cgagccaggc cttcgcgccg cacttcccgc acacggtgcg
     1381 ccggtcggaa accaagcaat gttcggactg ccacttgtcg gcggccgacg acaataatgc
     1441 gatcatgtcg cagctgctgc tgctcggcac caatttcgtc aattttgtcg ggctcaacgt
     1501 ctggtcgggg atggaggacg gtttccaggc gacccgcgtc accgaatggg acgagccgca
     1561 ggcggtgatc ggcagctacc tccagaaata tgcctatccc gactattgga agctgcacgt
     1621 cgaccagaat gggcgcgagc tcaagaactg ggtgcgcggc aagatcctcg acaagaaatt
     1681 gtcgggcgaa acccacgcgc tcgaggaatt cgccaatatc gtccaggaca cgagcgggcg
     1741 ggtgaactgc ctccagcagc gcggcgaata tatgtatgtg gccgaaggca agggcggttt
     1801 ccgcgtctat gatgtcgcgt cgatcggcaa caagggcttc tccgaacgca tcgtccgcgc
     1861 gcccttctcg ccgatgggcc acgatacgca tgtgaagacg aagaacgcca cctgcatggc
     1921 gatcgcgacg acgcagccga tcagcgtgtc gcgcaacgag actatcgtga agaatttccc
     1981 cgagaaccac gaacaggtga tgcacgacat ctatcgttac gccgtgatca ccgacagcgt
     2041 cgaggggctg gtgctcgtcg acatcgacac gatggccgac ggcgagttcc gcaacaacaa
     2101 gctgaagcgc gcgctgacgt ggaacgaggg caatgtcctc gcgggggcgc ggcacatcac
     2161 gctcgcgggc agcatcgcct atgtcaccac cgacgccggg ctcgtcgtcc tcgacctgac
     2221 catcccgctc cagccgaagg tgaccgcgca actgccgctg accgatgcgc gcgcgagcgc
     2281 ggtgcagttc cgctatctct gggtcaccga tgccgagggg gtgaagctgt tcgacgtcac
     2341 cgacctcgcg cagccggtcg cggtgcccgc gggcacggtg cggctcgcca acgcgcgcaa
     2401 aatctatctc gcgcgcacct atatgtatgt cgcggcaaag gccgacgggc tggtgatcgt
     2461 cgatgtgacg cgccccgcgg cgccgatcgt ctggccgggg ctgaccttcg gcggcgcgat
     2521 gaccgatgcc gaggatgtga tcgtcgcgtc gaccaacgca tcgctgttcg gctatgtcgc
     2581 cgacgggcgg ggcgggatca aggtgctcca gctcaccagt cccgacaatc cggggctcta
     2641 cggcttttcg cccaagccga cgcccgagct gatcgcctgg gcgaagacgc cctcgcccgc
     2701 gctcgcgctg tcgaaggggc tcgaccgcga ccgcgcggtc gacgagacgg gcggccagat
     2761 ggcggtgttc ggccgcctcg gctcgcgccc cttcacgcgc ggcgagatgg agaagctgtt
     2821 cctcaacagc aagggggcgg tctacaaggt tcgcgacgag gtcgaccaaa gcgcgtggcg
     2881 gccgccacta ttatacgcaa actgaggaag ctggtctgct gccgaccgga aagcaggcag
     2941 ccgcccaaaa gaaaaccggc gcggatcgct ccgcgccggc ttccctcggt agttctcgaa
     3001 gcgcggtgcg tcaggcgacg cggcgcacct cattgccttc gtcgtcgatg tcgcgaagga
     3061 cataaccgcg gccccagacc gtctcgatgt aattttcgcc gccgcacgcg agcgcgagct
     3121 tcttcctgag cttgcagatg aagacgtcga tgatcttcaa ttcgggctcg tccatcccgc
     3181 catagaggtg gttgaggaac atttccttgg tcagcgtcgt gcccttgcgc aggctcagga
     3241 gctcgagcat ctgatattcc ttgccggtca gatgcacgcg cgtcgtgtcg acttcgaccg
     3301 tcttggtatc gaggttaacc gcgagcttgc cggtcttgat gacgctctgg ctgtggccct
     3361 tcgagcggcg gacgaccgcg tggatgcgcg cgaccagttc gtcgcggtgg aacggcttgg
     3421 tgacataatc gtcggcgcca aagccgaacg agcgcacctt cgaatccatt tccgaaatgc
     3481 ctgacaggat cagcaccggc gtctgcacct tcgcggtgcg cagctttttc agcacgtcat
     3541 agccgtgcat gtcgggcagg ttcaggtcga gcaggatgat gtcgtaatca tagagcttac
     3601 ccaggtccaa gccttcttcc cccagatcgg tggtatagac gttgaagcct tcggtcgtga
     3661 gcatcgtatc gatcgccttc gcggtcgtcg gctcgtcctc gatcagcagt acgcgcattg
     3721 ggcacccctt tttcccacga taatcccgcg aaccgatcgc atgaccatca aggatgtcac
     3781 cgaccaaccc ccgttgcagg actgccggaa cattaaccat gaaagcaatg aagggaaaag
     3841 gttaattttg atttaacccg cggaactccg cgagtcgcgc tctatttgca acagtcggcc
     3901 aggcatgcct catcctttat gcagcgccag atattggtcg tgcgcgccgg tgcaggagat
     3961 tttcagcgtc tcgacaagat ggtccgaaag cgcccgcacg cgcgccgggc gcagccgcga
     4021 cggcggcgac aacaggtgca gatgcgactg cggcggcgac caatcggtga ggatcggaac
     4081 gaccgcgccc gacgccagcg cgtcggcggc gatgaagtcg ggcaagagcg cgatcccgac
     4141 cccagcgacc acgagcggca ccgcgatatc gccattgttc gcgaacaagg ggcccgtcgg
     4201 cagcaccgtc gcttcctcgc ccttgcggtg gaaatggagc ggcatcgcgc gctggcggtg
     4261 gccgtacccg atcagccggt ggtccgcgag gtcgagcgga tgcttgggga ccccgtgctt
     4321 ttccagatag gcggggctcg cgatcaccga cgccgcgacc ggcgcgatcg tccgcgcgag
     4381 caggctcgaa tcggcgagcg gcgcgatgcg caaggtcagg tcgattcctt ccgcgacggg
     4441 gtcgttgcgc gcatcgctca gcagcacctc gatctcgacc gccggatggc ggtcgaggaa
     4501 ggctgcgagc ggcggcccga gcacctt
//
LOCUS       DATMSU010000126         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_172913,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000126 DATMSU010000000
VERSION     DATMSU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172913"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..>1987)
                     /gene="polA"
                     /locus_tag="VN034_03395"
     CDS             complement(86..>1987)
                     /gene="polA"
                     /locus_tag="VN034_03395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWV59673.1"
                     /translation="LPRASAATATPAAGPSTPALPAMPPIDRALYETVTTIEALDRWI
                     EEARAAHVVAIDTETASLDSVTGTLVGVSMATGPGRACYIPLGHGGTDMFAEKPEQVP
                     LADAIGRLHALFSDEAVLKVGHNLKYDIGVLAQHGITIAPYDDTLVMSFALDAGKHQH
                     GLDELAKLHLDHVCISFKEVCGTGKSQISFAEVPLDRATQYAAEDAEVAWRLWKLLKL
                     RLPIEGGTRVYEMVDRPLAAVVESMERAGIMVNRDYLARLSGEFANDMLRMEGEIHGL
                     AGQPFAIGSPKQLGEILFDKMGLKGGRKGKSGDWSTDQSELERLERDGVPIARMILEW
                     RQLAKLKSTYTDALQQQINPTTGRVHTSYSLVGAQTGRLSSTDPNLQNIPIRTETGRQ
                     IRDAFIAAPGHVLVAADYSQIELRLAAHMADVPELKEAFAQGQDIHAATAIELFGEVN
                     RDTRGKAKTVNFSILYGISRWGLAGRLEVTPDEAQALIARYFERFPGISDYITDTLDN
                     ARAKGYTETLFGRKTWFPRIKAANQNERAGSERAAINAPIQGTSADLIKRAMARMPKA
                     LADAGLSDVRMLLQVHDELVFEAPEDKAAAAGDVIRAVMSGAAEPALKLSVPLEVEVG
                     IGKSWGEAH"
BASE COUNT          297 a          647 c          679 g          364 t
ORIGIN      
        1 ggcgtggagc cggggggcgc cgccgtctcc ggcggcacgg cagcggtctc ggcgacgagc
       61 gccgcgacga acaacagtgc gccgatcaat gcgcttcgcc ccagctcttg ccgatgccga
      121 cctcgacctc cagcggaacc gacagcttga gcgccggttc ggcggcgccc gacatcaccg
      181 cgcggatgac gtcgccggcg gccgcggcct tgtcctcggg ggcttcgaac acgagttcgt
      241 cgtgaacctg cagcaacatg cggacgtcgg aaagccccgc gtccgctaat gccttcggca
      301 tccgcgccat cgcgcgtttg atcaggtcgg cgctggtgcc ctggatcggc gcgttgatcg
      361 cggcgcgttc gctgcccgcg cgttcgttct ggttcgcggc cttgatgcgc gggaaccagg
      421 ttttccgccc gaacaaagtc tcggtataac ccttggcgcg ggcgttatcg agcgtgtcgg
      481 tgatatagtc cgaaatgccg ggaaaccgct cgaaatagcg cgcgatcagc gcctgcgcct
      541 cgtcaggcgt cacttcgagc cggcccgcga ggccccagcg cgaaatgccg tagaggatcg
      601 agaaattgac cgtcttcgcc ttgccgcgcg tgtcgcgatt gacctcgccg aacaattcga
      661 tcgcggtcgc ggcgtggatg tcctgtccct gcgcgaaggc ttccttgagt tcggggacat
      721 cggccatatg cgccgccagc cgcagttcga tctggctata gtcggcggcg acgagaacat
      781 ggccgggcgc cgcgatgaag gcgtcgcgga tctggcggcc ggtctcggtg cggatcggga
      841 tattctggag attggggtcg gtcgacgaca gccgcccggt ctgcgcgccg acgaggctgt
      901 agctcgtatg gacacggccg gtcgtcggat tgatctgttg ttgcagcgcg tcggtatagg
      961 tcgacttgag cttcgcgagc tggcgccatt ccaatatcat ccgcgcgatc ggcaccccgt
     1021 cgcgttcgag ccgttcgagc tcgctctggt cggtcgacca gtcgcccgac ttgcccttgc
     1081 gcccgccctt gagacccatc ttgtcgaaca atatttcgcc gagctgcttg gggctgccga
     1141 tcgcgaaggg ctggcccgcg aggccgtgga tttcgccctc catgcgcagc atgtcgttcg
     1201 cgaactcgcc cgacagccgc gcgagatagt cgcggttcac catgatgccg gcgcgttcca
     1261 tgctctcgac cacggcggcg agcgggcggt cgaccatttc atagacacgc gtgccgcctt
     1321 cgatcgggag acggagcttc agcagtttcc acaaccgcca cgcgacctcg gcatcctcgg
     1381 cggcatattg cgtcgcgcgg tcgagcggaa cctcggcgaa gctgatctgc gactttccgg
     1441 tcccgcacac ttccttgaac gagatgcaga catggtcgag gtggagcttg gcgagctcgt
     1501 cgagcccgtg ctgatgcttg cccgcgtcga gcgcgaagct catcaccagc gtgtcgtcat
     1561 aaggcgcgat ggtgatgccg tgctgcgcaa gcacgccgat gtcatatttg aggttgtgcc
     1621 cgaccttgag caccgcctcg tcggagaaaa gcgcatgaag ccgtccgatc gcgtcggcga
     1681 gcggcacctg ctcgggcttt tcggcgaaca tgtcggttcc gccgtggccg agcgggatgt
     1741 agcaggcgcg ccccgggccg gtcgccatgc tgacgccgac gagcgtgccg gtgacgctgt
     1801 cgaggctcgc cgtctcggta tcgatcgcga cgacatgcgc ggcgcgcgct tcctcgatcc
     1861 agcggtcgag cgcctcgatc gtcgtcaccg tctcgtaaag cgcgcggtcg atcggaggca
     1921 tggcgggaag ggcaggggtc gaggggcccg cggcgggtgt cgctgtcgcg gcgctcgcgc
     1981 gcggcag
//
LOCUS       DATMSU010000127         4350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_77846, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000127 DATMSU010000000
VERSION     DATMSU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77846"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..730
                     /locus_tag="VN034_03400"
     CDS             17..730
                     /locus_tag="VN034_03400"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWV59674.1"
                     /translation="MFLADGRSPAASGPHPRRGHRWSPPGWGRGGTLACYRKPGQDWT
                     IFEIDPVMVDIARDPAKFTFISDCAPDTPIVIGDARLQIAEQPAGHFDIIVIDAFSSD
                     AIPLHLLTKEAIGIYARALKPDGILLIHISNRFFGLEPVLAAEAKARGWTAAIRMDPG
                     PAGDEYGDLTGSNWVALTATAGRMRELTGGIRPRKDSRDDGAWVPLDARPGFTRWTDD
                     YASTLPVLIWKNIIGGRDE"
     gene            727..1281
                     /locus_tag="VN034_03405"
     CDS             727..1281
                     /locus_tag="VN034_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWV59675.1"
                     /translation="MTYQDVSIISVNGKTPQIDPSAFIAPGCRIIGDVTIGADVSIWY
                     NCVLRADVSRIVVGARSNIQDGSVVHCDGPMPHHPEGFPTIIGEDVLIGHLAMVHGCT
                     LADRAFVGLKATVMNGCRIGSDAMLAAGALLTENKEIPERELWAGSPARRVREIGDAQ
                     AAGMQLGVAHYVMNGRMHKAAIEG"
     gene            complement(1428..2000)
                     /locus_tag="VN034_03410"
     CDS             complement(1428..2000)
                     /locus_tag="VN034_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HWV59676.1"
                     /translation="MDKFFGNLHAVLGTGLVLAIILMLCLNGQNFEDGGAAAGAVMRW
                     LHTFFGVLWIGLLYYFNFVQMPTMPKIPAELKPAVGKHIAPAALFWFRWAALVTVLLG
                     IGIAGHAKYLAPALGLQDPYKLIGVGMWLGLIMAFNVWFLIWPNQKKALGIVEADDAA
                     KAKAARTAMIFSRVNTLLSIPMLYAMVNFS"
     gene            complement(2110..2883)
                     /locus_tag="VN034_03415"
     CDS             complement(2110..2883)
                     /locus_tag="VN034_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfur carrier protein/thiazole
                     synthase protein"
                     /protein_id="HWV59677.1"
                     /translation="MSADQWSVAGRTFTSRLIVGTGKYKDFEQNAAALEASGAEIVTV
                     AVRRVNVSDPAAPMLTDYIDPKKVTYLPNTAGCFNADDAIRTLRLAREAGGWDLVKLE
                     VLGEAKTLYPNMRETLEATEVLAKEGFLPMVYCVDDPIAAKQLEDAGAVAIMPLGAPI
                     GSGLGIQNRVTIRLIVEGASVPVLVDAGVGTASDAAVAMELGCDGVLMNTAIAEAKDP
                     ILMARAMKRAVEAGRDAYLAGRMGVRRYADPSSPLAGLI"
     gene            complement(2885..3088)
                     /gene="thiS"
                     /locus_tag="VN034_03420"
     CDS             complement(2885..3088)
                     /gene="thiS"
                     /locus_tag="VN034_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWV59678.1"
                     /translation="MSISIILNGEPRQVREGSIADLVASLGLDIKKVAVERNRDIVPR
                     STLADVALAEGDALEIVHFVGGG"
     gene            3072..3383
                     /locus_tag="VN034_03425"
     CDS             3072..3383
                     /locus_tag="VN034_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59679.1"
                     /translation="MIEIDMGVRLPLSPQNTICRACFLPYNASMTSLEKFISFAQALP
                     ADQLEAVEASLSALMESLSEQYDFSPEELAEIDRRVADPDPEFSNKADIARIFGQPFS
                     A"
     gene            3380..3658
                     /locus_tag="VN034_03430"
     CDS             3380..3658
                     /locus_tag="VN034_03430"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWV59680.1"
                     /translation="MMTILFRKEAEADLRSIIAYYEEIAPDTLPHILSDIYRSIDQLS
                     RYPHSGMQVPDRSFRRIVTIKYHFKLAYEASEGRVVILGIFRYQDRES"
     gene            3692..4132
                     /locus_tag="VN034_03435"
     CDS             3692..4132
                     /locus_tag="VN034_03435"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWV59681.1"
                     /translation="MPELPTVFVLSGPNLNLLGTREPEIYGHDTLNDIHARLEAQAEG
                     LGIKLECRQTNHEGVLIDWLHEAYIAGAKAVLLNAGGYTHTSIAIHDAIKSIKVPVIE
                     VHLSDPMQRETFRHISYVGMAAAAHFAGHGADSYTLALDAAARL"
     gene            4202..>4350
                     /locus_tag="VN034_03440"
     CDS             4202..>4350
                     /locus_tag="VN034_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, biotin carboxyl carrier
                     protein"
                     /protein_id="HWV59682.1"
                     /translation="MGDHKDNGINIDPAFVRALAELLDDTQLSEIEVEDGDRKVRVAR
                     TLTAAA"
BASE COUNT          819 a         1434 c         1343 g          754 t
ORIGIN      
        1 tccttcttgt tccccaatat tccttgcgga cggaagatca ccagcagcat cagggccaca
       61 cccacgacga ggccaccgat ggtctccacc tggctggggg agagggggga cgctcgcctg
      121 ctaccggaag cccgggcagg actggacgat cttcgagatc gatccggtga tggtcgacat
      181 cgcgcgcgat cccgccaaat tcaccttcat ctccgactgt gctcccgaca cgccgatcgt
      241 catcggcgac gcgcggctcc agatcgccga gcagcccgcg ggccatttcg acatcatcgt
      301 catcgacgcc ttttcgtcgg acgcgatccc gctccatctg ctgacgaagg aagcgatcgg
      361 catctatgcg cgggcgctga agcccgacgg catcctgctc atccatatct cgaaccgctt
      421 cttcgggctc gaacccgtgc tcgcggcgga ggcgaaggcg cgcggctgga ccgccgcgat
      481 ccgcatggat cccggcccgg cgggcgacga atatggcgat ctgaccggat cgaactgggt
      541 cgcgctgacc gcgacggcgg gccggatgcg ggaattgacc ggcggcatcc gtccgcgcaa
      601 ggatagccgt gacgacggcg cctgggtccc gctcgacgcg cgccccggtt tcacccgctg
      661 gaccgacgat tatgcctcga cgctgccggt cctcatctgg aagaatatca tcggaggccg
      721 cgacgaatga catatcagga cgtcagcatc atcagcgtca acggcaagac gccgcagatc
      781 gaccccagcg ccttcatcgc gcccgggtgc cgcatcatcg gcgacgtgac gatcggcgcg
      841 gacgtcagca tctggtataa ttgcgtgctg cgcgccgacg tcagccgcat cgtcgtcggc
      901 gcgcgatcga acatccagga cggcagcgtc gtccattgcg acggcccgat gccgcatcac
      961 cccgaaggct ttccgacaat catcggcgag gatgtgctga tcggccatct tgcgatggtc
     1021 cacggctgca cgctcgccga ccgcgcgttc gtcggcctga aagcgacagt gatgaacggg
     1081 tgccgcatcg gcagcgacgc gatgctcgcc gcgggggcgc tgctgaccga gaataaggaa
     1141 attccggagc gcgaactctg ggccggatcg cccgcgcgcc gcgtgcgcga gatcggcgat
     1201 gcgcaggcgg cgggcatgca gctcggcgtc gcgcattatg tgatgaacgg ccgcatgcac
     1261 aaggcggcga tcgaggggtg aatcaatcct ccctgtgccg aaggcatggg gagggggacc
     1321 gctcgcgaag cgagtggtgg aggggccgca acggcgcgtt atggcccctc cgtcagccct
     1381 tcgggctgcc acctccccat cgcttcgcga cagggaggat ggttcgatca gctgaaattc
     1441 accatcgcat agagcatcgg gatcgacagc agcgtattca cccgcgaaaa gatcatcgcg
     1501 gtccgcgccg ccttcgcctt ggccgcgtcg tcggcctcga cgatgcccag cgcctttttc
     1561 tggttcggcc agatcaggaa ccagacgttg aacgccatga tcaggccgag ccacatgccg
     1621 accccgatca gcttgtacgg atcctgcagg cccaatgccg gcgcgagata tttggcgtgg
     1681 ccggcgattc cgatccccag cagcaccgtg accagcgccg cccagcggaa ccagaagagc
     1741 gcggcgggcg cgatatgctt gccgaccgcg ggtttcaatt cggccggaat cttgggcatc
     1801 gtcggcatct gaacgaagtt gaaataataa agcaggccga tccacagcac gccgaaaaat
     1861 gtatgcagcc agcgcatgac cgctccggcg gccgcgccgc catcctcgaa attctggccg
     1921 ttgaggcaca gcatcaggat aatcgcgagc acaaggcccg tgccgagcac ggcgtgcagg
     1981 tttccgaaaa acttgtccat ggcagttccc ccttcttata tcggcgccgt gcgacccggt
     2041 gcctgtgcgc cgatatgcca gcgacggggg aagggctcaa gcctgtcgga agcgggtcgt
     2101 ggtttgggcc tagatcaacc ccgccagcgg actcgacgga tcggcgtaac gacgaacgcc
     2161 catgcgcccc gccagatagg cgtcgcgccc cgcctcgacc gcgcgcttca tcgcgcgcgc
     2221 catcagaatc gggtccttcg cctcggcgat cgcggtgttc atcagcaccc cgtcgcagcc
     2281 gagttccatc gcgaccgcgg cgtcgctcgc cgtcccgacg ccggcatcga cgagcacggg
     2341 caccgacgcc ccctcgacga tcaggcggat cgtcacgcgg ttctggatgc cgagtcccga
     2401 cccgatcggc gcgccgagcg gcatgatcgc gaccgcgccc gcatcctcaa gctgcttcgc
     2461 cgcgatcgga tcgtcgacgc aatagaccat cggcagaaag ccctctttcg cgagcacctc
     2521 ggtcgcctcg agcgtctcgc gcatgttcgg atagagcgtc ttcgcctcgc cgagcacctc
     2581 gagcttgacg agatcccagc cgcccgcttc gcgcgcgagc cgcaaggtgc ggatcgcgtc
     2641 gtcggcgttg aagcagcccg cggtgttggg gaggtaagtg accttcttgg ggtcgatata
     2701 gtcggtgagc atcggcgccg cgggatcgga cacattgacg cggcgcaccg cgacggtgac
     2761 gatctcggcc cccgacgctt cgagggcggc ggcgttctgt tcgaaatcct tgtatttgcc
     2821 ggtgccgacg atcagccgcg acgtaaatgt ccggccggcg acgctccact gatccgctga
     2881 caactcaacc gcctcctacg aaatgaacga tttcgagcgc atcgccctcg gcaagcgcca
     2941 cgtcggcgag cgtcgagcgc ggcacgatgt cgcgattgcg ctcgaccgcg accttcttga
     3001 tgtcgaggcc gagcgacgcg acgagatcgg cgatgctgcc ttcgcgcacc tgccgcggtt
     3061 cgccgttgag gatgatcgag atcgacatgg gagttcgctt accgctttcg ccgcaaaaca
     3121 ccatatgccg cgcgtgtttt ttgccctaca atgcgagcat gacctcgctc gaaaagttca
     3181 tctccttcgc gcaggccctg cctgccgatc aacttgaggc ggtcgaggcc tccttgtccg
     3241 ctctgatgga atccttgtcc gagcaatatg atttttcacc ggaagaactg gctgaaatcg
     3301 accggcgcgt ggcggatccc gaccccgaat tttcaaacaa ggcggacatc gcgaggattt
     3361 tcggacagcc cttctcggca tgatgacgat cctgttccgc aaggaagcgg aagccgatct
     3421 gcgatccatc atcgcatatt atgaagagat cgcgcccgac acgcttccgc acatcctgtc
     3481 ggacatatat cgctcgatcg accagctttc ccgttatcct cattcgggca tgcaagtgcc
     3541 ggaccgatcc tttcgccgca tcgtcacgat caaatatcat ttcaaactgg cttatgaggc
     3601 gagcgagggc cgggtcgtta tcctcggcat ctttcgctat caggatcgcg agtcataacg
     3661 ccacatcccg ccgcaaccga aagccaagcc tttgcccgaa cttccgaccg tctttgtcct
     3721 ctccggcccc aatctcaacc tgctcggcac gcgcgagccc gagatctatg gtcacgacac
     3781 gctgaacgac atccacgcgc ggctcgaagc gcaggcggag gggctgggga tcaagctcga
     3841 atgccggcag acgaaccatg aaggcgtgct gatcgactgg ctgcacgaag cctatatcgc
     3901 gggcgccaag gcggtgctgc tcaacgcggg gggctatacg catacgtcga tcgcgatcca
     3961 cgacgcgatc aagtcgatca aggtgccggt gatcgaggtc catctgtcgg acccgatgca
     4021 gcgcgagacg tttcgccaca tcagctatgt cggcatggcg gcggcggcgc attttgccgg
     4081 gcatggcgcg gacagctata cgctggcgct ggacgccgcc gcgcgtctct gacaataggg
     4141 cgcatcaaaa ataggggtca gggcgttgca atagcgacgc cgcagcaaca ggaaaattct
     4201 catgggtgac cataaagaca atggcatcaa cattgatccg gcgttcgtcc gcgcgctcgc
     4261 cgagctgctc gacgacacgc agcttagcga aatcgaggtc gaggacggcg accgcaaggt
     4321 gcgcgtcgcg cgcacgctga ccgccgcggc
//
LOCUS       DATMSU010000128         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_87793, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000128 DATMSU010000000
VERSION     DATMSU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87793"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      351..535
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            679..2634
                     /locus_tag="VN034_03445"
     CDS             679..2634
                     /locus_tag="VN034_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59683.1"
                     /translation="MFKVCFRSSICLAAVAASSTAAADEAAHAVAADADSIIVTATRT
                     PLTLGEIPSSVAVLDKEAIDRAQDIGVTELLLRTPGISMSRNGGYGTSTSLRIRGAES
                     EHTVTVIDGVKLNDPSSTGGGFNFANLLAGDIDRIEVLRGPQSILWVSQAIGGVINIV
                     TASPEKTLEGSFDLEAGSRETVSARAAIGGRTGPLSWRLGGQRFSTDGISSHAKAFGG
                     VERDGYRNSNVSGRAELALADNVSAEVRGYYSSGRVEFDGFNTDSNDVGLNKEFVGYA
                     GLNFALADGRFRNRIAYAYTDTNRDNFNPDRERPQSFEADGENKRWEYQGSFDISDRI
                     TAIFGFENERSNFRSRSPSASLATPVPDFVRGKAEITSAYGQLSVEPVDGLTLNGGVR
                     YDDHSRYGGQTLFAAGGVWRLPTGTVLRASYGEGFKAPGLYQLFSEYGNVGLDPEEAR
                     GWEAGIEQHLLSDKLVVGATWFDRTTTNQIIYNGCSSPTTDPLCTVPGSNPPVPRFGY
                     YLNVARSEAHGVEAAAALTLGGLTLDGNYSWIVAEDRSEDSDSFGNWLPRRPRHSANA
                     SASYAFGFGLELGAALRWSGKSYDNASNARRLDDYTLVDLRAEFALSDAVKLFARAEN
                     IFDEEYMTAYRYGSLGRSIYAGIRGRF"
BASE COUNT          479 a          893 c          903 g          442 t
ORIGIN      
        1 gtttcgcggt agcggccccg cccgcgatgc tggtgcccga ccgcccgagc gcggcgcagc
       61 gcgaattgcc ggggctgttt tagcaccggc gggaacgcaa atcccttccc ccttgatggg
      121 ggaaggatat gcagccttat cgcgaagcga ttaggcgaag ttggatgggg gtgagggtcg
      181 ccctgcacca tcgcgtcaga ccaagactgc acccccaccg ctgcgactag cgaacaagtt
      241 cgcaagtctc gctgcctccc ccatcgaggg ggagaggaat tcatgttctc gacaatgacc
      301 atgatttctc tccatcgcgg caattgaccg caccccagga atgaggctaa cggccgccgc
      361 aagcgacagg tgccccggcg acggggacta agagggaacg ggaaaacccc ggctgcccct
      421 gcaactgtac gcggcgagcc atcggccaca tgccattggg accccggtcc cgagaaggcg
      481 gccgaccggc gctgacccgc aagccaggag acctgcccgt cgccgtcgtc tttcgcgcgg
      541 acagggtgtg ccgggcggac gggggtgaac ccgcatgacg acaaagctgg ctgcgcaggt
      601 gcgcgggcga tgtcgtgcgt cccgaaagga cgaccgatcg gccgcgcgct tgggcggcat
      661 tgtcgatggg tttgtccaat gttcaaagtc tgtttccgaa gttcgatctg ccttgccgcc
      721 gttgcggctt cctccaccgc cgccgccgac gaagccgcgc acgccgttgc cgccgacgcc
      781 gacagcatca tcgtcaccgc gacgcgcacg ccgctgacgc tgggcgagat tccgtcgtcg
      841 gtcgcggtgc tcgacaagga agcgatcgac cgcgcgcagg atatcggcgt caccgaattg
      901 ctgctgcgca ccccggggat cagcatgtcg cgcaacggcg gttacggcac ctcgacctcg
      961 ctgcgcattc gcggcgccga gtccgaacat acggtgacgg tgatcgacgg ggtcaagctc
     1021 aacgatccct cgtcgaccgg cggcggcttc aacttcgcca atctgctcgc cggcgacatc
     1081 gaccggatcg aggtgctgcg cgggccgcag tcgatcctgt gggtcagcca ggcgatcggc
     1141 ggcgtgatca acatcgtcac cgcatcgccc gaaaagaccc tcgaaggcag cttcgacctc
     1201 gaggcggggt cgcgcgagac ggtgagcgcg cgcgccgcga tcggcggccg caccggaccg
     1261 ctgagctggc gcctcggcgg ccagcgattc tcgaccgacg gcatttcgtc gcacgccaag
     1321 gcgttcggcg gggtcgagcg cgacggctat cgcaacagca atgtttcggg gcgcgccgaa
     1381 ctggcgctgg ccgacaatgt cagcgccgag gtgcgcggat attactccag cggccgcgtc
     1441 gagttcgacg gcttcaacac cgacagcaat gatgttgggc taaacaagga attcgtcggc
     1501 tatgcggggc tcaatttcgc cctcgcggac gggcggttcc gcaatcgaat cgcctacgcc
     1561 tataccgaca ccaaccgcga caatttcaac cccgaccgcg aacggccgca gagcttcgag
     1621 gccgacggcg agaacaagcg ctgggaatat cagggcagct tcgacatcag cgaccggatc
     1681 accgcgatct tcgggttcga gaatgagcgg tcgaacttcc gcagccgctc gccttcggcg
     1741 tcgctcgcca ccccggttcc cgatttcgtg cgcggtaagg ccgaaatcac cagcgcttac
     1801 ggccagctca gcgtcgaacc ggtcgacggt ctgacgctga acggcggcgt ccgttacgac
     1861 gatcatagtc gctatggcgg gcagaccttg ttcgccgcgg gcggcgtgtg gcggctaccg
     1921 acgggaacgg tgctgcgcgc gagctatggc gaggggttca aggcgcccgg gctctatcag
     1981 ctgttcagcg aatatgggaa tgtcgggctc gaccccgagg aggcgcgtgg ctgggaagcg
     2041 ggaatcgagc agcatctgct gagcgacaag ctggtcgtcg gcgcgacctg gttcgaccgc
     2101 acgacgacga accagatcat ctataatggc tgctcgtcgc cgacgaccga tccgctctgc
     2161 accgtgccgg gcagcaaccc gccggttccg cgcttcggct attatctgaa cgttgcgcgc
     2221 agcgaggcgc atggcgtcga agccgcggca gcgctgacgc tcggcgggct gacgctcgac
     2281 ggcaattata gctggatcgt cgccgaggac cgctccgaag acagcgacag tttcggcaat
     2341 tggctgccgc gccgtccgcg ccacagcgcc aatgcgtcgg cgagctatgc cttcgggttc
     2401 gggctcgaac tcggcgcggc gctgcgctgg tcgggcaaaa gctatgacaa tgcgagcaac
     2461 gcccggcggc tcgacgatta tacgctcgtc gacctccgcg ccgaatttgc gctctccgac
     2521 gcggtgaagc tgttcgcgcg cgccgagaat atcttcgacg aggagtatat gaccgcctat
     2581 cgctacggct cgctcggccg cagcatctat gccggcatca gggggcgttt ctgatggcgg
     2641 cgttgtcgaa ccgcagcgcg tcgacgctct gggtcgcgct gctcaccgcg gcgagcacgg
     2701 cgacgacgct cgcgctc
//
LOCUS       DATMSU010000129         5162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_182676,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000129 DATMSU010000000
VERSION     DATMSU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182676"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VN034_03450"
     CDS             complement(<1..336)
                     /locus_tag="VN034_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59684.1"
                     /translation="MRRWGFLFMMAMATPVAALAAPPVALGIFDGWGAFRDPATPRCY
                     AIAAPAATIGTPRVKPYASVGYWPKSRIRGQFYVRLSKARAPDRELRLTIGSRRFILT
                     GNGAHGWASD"
     gene            508..1623
                     /gene="dnaN"
                     /locus_tag="VN034_03455"
     CDS             508..1623
                     /gene="dnaN"
                     /locus_tag="VN034_03455"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HWV59685.1"
                     /translation="MKATIERAVLLKSLGHVQSVVERRNTIPILSNVLIEADASGQLK
                     LMATDLDLQVVETIAAKVETPGTTTVSAHTLFEIARKLPEGSEVSLAAAEGKMQIKAG
                     RSNFNLPTLPRDDFPVIAEGDLPTNFELPVAELIQIIDKTRFAISTEETRYYLNGIFF
                     HVAEDATGPVLKAAATDGHRLARYTVTRPDGAASMPDVIVPRKCVGEIRKLLDEAEGN
                     VEISLSASKIRFQLGHAVLTSKLIDGTFPDYSRVIPTANDKLLKVDPKSLFQGVDRVS
                     TIASEKTRAVKVGLDKDRITLSVTSPENGTAAEELAAGYDSDAMEIGFNARYLSDILG
                     QVDGDSVELHLADANAPTLIRESEKSPALYVLMPMRV"
     gene            complement(1688..2005)
                     /locus_tag="VN034_03460"
     CDS             complement(1688..2005)
                     /locus_tag="VN034_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59686.1"
                     /translation="MSGINHHRKGPPRPGSSSMDHPRGLQRAWIAPQEKAKKAAEREN
                     DERIRKAHFRLLNKAVRLEPLGVELEQRRARGEHLSFFDKALLEKWRTIKGVFEGTFP
                     TCD"
     gene            complement(2002..3363)
                     /locus_tag="VN034_03465"
     CDS             complement(2002..3363)
                     /locus_tag="VN034_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HWV59687.1"
                     /translation="MRKILKYAALALLVLLIAGGITLYVMSRPDVARFSTAELSGRVP
                     VMASQRTETFPTVNVPEATSWPAGQAPRAAQGLTVARFAEGLDHPRTMLVLPNGDVLA
                     AESQSPPRKDGGVQGAVMKSLMGKGGAGRRPSANRITLLRDADGDGKAEVKSAYITGL
                     NSPYGMALVGDTLYVANTDALLAFPYVAGETKMSGKPVKVVDLPAKGTNRHWTKSLVA
                     APNGWLYIGVGADSNIGESGMNREFRRASVLEVRPENKYMRTFAAGIRNPVGLAFYPG
                     TDRLWTVVNERDMLGSDLVPDYLTDLDEGDFYGWPWYYWGGFIDPRVEPEAEDRRQYV
                     KRPEYGLGAHTAPLGMTFTEGLDLGERWANGALIARHGSWNREPVTGYDVVFVKFDAN
                     GKPARALPITLLDQFLGKDGKTTRGRPADVKVAKDGSALVADDTGGVIWRVTKANPNR
                     ESE"
     gene            3535..3750
                     /locus_tag="VN034_03470"
     CDS             3535..3750
                     /locus_tag="VN034_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59688.1"
                     /translation="MHLPAPFLTDRAAVEDAHALITLHGEEAGFAAAARAEQYRALGN
                     HLHFARWRQIERLITYLSVEDILDTVH"
     gene            complement(3754..4680)
                     /gene="argC"
                     /locus_tag="VN034_03475"
     CDS             complement(3754..4680)
                     /gene="argC"
                     /locus_tag="VN034_03475"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWV59689.1"
                     /translation="MTQTVFIDGAAGTTGLEIAERLAGRSEFSLITLGEARRKDAAAR
                     REALNDADFVILCLPDDAAREAVAMIGNDRTRVIDASTAHRVAPGWVYGFPEVSGHDA
                     VAAATRVSNPGCYSTGFIALVAPLVRAGLLPADWPYICHAVSGYSGGGKALIERFEQD
                     SDIAWRGYALTLGHKHVPEMQARCGLSIGPLFSPAVIPAHRGMIVEVPLPLNAMPGAA
                     SPDKLRAALADFYAGSPIVVMDEAPADGEILLRASDAGDDRIALSVFANADASQARLV
                     ARLDNLGKGASGACVQNLNIMAGLPETAGLRL"
     gene            complement(4686..>5162)
                     /locus_tag="VN034_03480"
     CDS             complement(4686..>5162)
                     /locus_tag="VN034_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HWV59690.1"
                     /translation="LPRGALVMGEVEGEWLATSHGYLPLAALAEVTTGDSDPAELAEQ
                     MIGTPYLWGGRTAKGIDCSGLVQLVWGAAGIQIPRDSDLQLAALGADKDVDPAALARG
                     DLVFFPGHVGIMADEKNIIHASRRWMAVKAEPLADVIARSAAKDHDPPVSGYKRLR"
BASE COUNT          909 a         1674 c         1683 g          896 t
ORIGIN      
        1 gtcgctcgcc cagccgtgcg cgccgttgcc ggtcaggatg aaacggcggc tgccgatcgt
       61 caggcgcagc tcgcggtcgg gcgcgcgcgc tttcgacagg cggacataga attggccgcg
      121 gatgcgcgat ttcggccaat agccgacgct cgcatagggt ttcacgcgcg gcgtgccgat
      181 cgtcgcggcg ggcgccgcga tagcatagca gcgcggcgtg gcgggatcgc gaaatgcccc
      241 ccagccgtcg aaaatgccga gcgcgaccgg tggcgcggcg agcgccgcga cgggcgtcgc
      301 catcgccatc atgaaaagaa agccccaccg ccgcatcgcg cttcgttcgc gcgattgccg
      361 ccgctttgca agcatggctt gcgcccgccg acgcgtgcgt taaggaaggt ggacattttc
      421 aggggccaga atcggggcgc gaatcgcctc cgatggtctg tccctcaggg acttttcccg
      481 cggaaagcac agggatggcg atttagaatg aaagcgacga tcgaacgcgc agtgttgttg
      541 aagagcctcg gccacgtcca gtcggtggtc gagcggcgca acacgatccc gatcctctcg
      601 aacgtcctga tcgaagccga cgcgtcgggc caattgaaac tgatggcgac cgacctcgat
      661 ctgcaggtcg tcgaaacgat cgcggcgaag gtcgagacgc cgggcacgac gaccgtgtcg
      721 gcgcacacgc tgttcgaaat cgcgcgcaag ctgcccgaag gatcggaagt cagcctcgcc
      781 gccgccgaag gcaagatgca gatcaaggcc ggccgttcga acttcaacct gccgacgctg
      841 ccgcgcgacg atttcccggt gatcgccgag ggcgacctgc cgaccaattt cgagctgccg
      901 gtcgccgagc ttatccagat catcgacaag acgcgcttcg cgatctcgac cgaggaaacg
      961 cgctattatc tgaacggcat cttcttccac gtcgccgagg atgcgacggg tccggtgctg
     1021 aaggcggcgg cgaccgacgg ccatcgcctc gcgcgctaca ccgtcacgcg ccccgacggt
     1081 gccgcgtcga tgcccgacgt catcgtgccg cgcaaatgcg tcggcgagat tcgcaagctg
     1141 ctcgacgagg ccgagggcaa tgtcgaaatc agcctctcgg ccagcaagat ccgtttccag
     1201 ctcggccatg cggtgctgac ctcgaagctg attgacggca ccttccccga ctatagccgc
     1261 gtgatcccga ccgcgaacga caagctgctc aaggtcgatc cgaagagcct gttccagggc
     1321 gtcgaccgcg tgtcgacgat cgcgagcgaa aagacgcgcg cggtcaaggt cgggctcgac
     1381 aaggatcgta tcaccctgtc ggtgaccagc cccgaaaacg gcaccgccgc cgaagaactc
     1441 gcggcgggtt atgacagcga cgcgatggag atcggtttca acgcccgcta tctcagcgac
     1501 atattggggc aggtcgatgg cgacagcgtc gaactgcatc tggccgacgc caacgccccg
     1561 acgctgatcc gcgaaagcga gaagagcccg gcgctgtacg tgctgatgcc gatgcgggtg
     1621 taataccctt gagggcaaaa aaagaccccg caattgcggg gtctttttgg ctgctttgcg
     1681 aaacttgtta atcgcaggtc ggaaacgtcc cttcgaacac acccttaatc gttcgccatt
     1741 tttctagtag cgccttatcg aaaaacgaaa gatgttctcc gcgcgcccgt cgttgttcaa
     1801 gttcgacacc caaaggttca aggcgcacag ctttattcaa aagtctaaaa tgcgcttttc
     1861 gtattctttc gtcattttca cgttcggccg ctttttttgc tttctcttgt ggagcaatcc
     1921 aagcgcgttg aagtcctcgt ggatgatcca tcgagcttga accgggtcgt ggcggccctt
     1981 tcctatggtg attgatgccg ctcactccga ttctcggtta ggattcgcct tcgtcacccg
     2041 ccagatcacg ccgccggtgt cgtcagcgac gagcgcgctg ccgtctttcg cgaccttgac
     2101 gtcggcgggg cgaccgcggg tggtcttgcc gtccttgccg agaaactgat cgagcaaggt
     2161 gatgggcagg gcgcgggcgg gtttgccatt ggcatcgaac ttgacgaaca ccacgtcata
     2221 gcccgtgacc ggttcgcggt tccatgagcc gtggcgcgcg atcaaggcgc cgttcgccca
     2281 gcgttcgcca agatcgaggc cttcggtgaa ggtcatgccg agcggcgcgg tgtgcgcgcc
     2341 gaggccatat tcggggcgct tcacatattg ccggcggtct tcggcctcgg gctcgacgcg
     2401 cgggtcgatg aagccgcccc aataatacca gggccagcca tagaaatctc cctcgtcgag
     2461 gtcggtcaga tagtcgggga cgaggtcgct gccgagcatg tcgcgttcgt tgacgaccgt
     2521 ccacagccgg tcggtgccgg ggtagaaggc gaggccgacg gggttgcgga tgcccgcggc
     2581 gaaggtgcgc atatatttat tctcgggccg cacctcgagc acgctcgcgc ggcggaactc
     2641 gcggttcatt cccgactcgc cgatgttcga atcggcgccg acgccgatgt aaagccagcc
     2701 attgggcgcg gcgacgaggc tcttggtcca gtggcggttg gtgcccttcg cgggcagatc
     2761 gacgaccttc accggtttac cgctcatctt ggtttcgccc gcgacatagg gaaaggcgag
     2821 cagcgcgtcg gtgttggcga catagagcgt gtcgccgacc agcgccatgc catagggcga
     2881 attgaggccg gtgatatagg cgctcttcac ctcggccttg ccgtcgccgt cggcgtcgcg
     2941 gagcagggtg atgcggttcg ccgacggacg gcgtccggcg ccgcccttgc ccatcaggct
     3001 cttcatcacc gcaccctgca cgccgccgtc cttgcgcggc gggctctgcg attcggcggc
     3061 gagcacatcg ccattgggca ggaccagcat cgtgcgcgga tggtcgaggc cctcggcaaa
     3121 gcgcgctacg gtaagcccct gcgcggcgcg cggcgcctgg cccgcgggcc aactcgtcgc
     3181 ctcgggtaca ttcaccgtcg gaaatgtctc ggtgcgctgc gacgccatca ccggcacccg
     3241 gccgctgagt tcggcagtcg agaagcgcgc cacgtcgggc cgcgacatga catagagcgt
     3301 gatgccaccg gcgatcagca ggaccagcag ggcgagggcg gcatatttca ggatcttgcg
     3361 catggggcct gtctacacgg gccgcgcgcg gcgtcaaagg ggttgcgcga gcgcgcgaag
     3421 ggttaaccgg cgatgctttg cgattaacct tacccgcctg ttcatcacgg ctggaaacgg
     3481 ggcggtgagg ccgggagcat agaaggaggc tccaagcaac ggaccaggaa gtcgatgcac
     3541 ctgccagccc cctttcttac cgatcgcgcc gcggtcgagg atgcccatgc gctgatcacc
     3601 ttgcatggcg aggaagccgg gttcgcggcc gccgcgcgcg ccgaacaata tcgcgcgctc
     3661 ggcaatcatc tccatttcgc gcgctggcgc cagatcgagc ggctgatcac ctatttgtcg
     3721 gtcgaggata ttctcgacac ggtgcactag gcgctacaag cgcaaacccg cggtttcggg
     3781 cagtcccgcc atgatgttga gattctggac gcaggcgccg ctcgcgccct tgcccagatt
     3841 gtcgagccgc gcgacgaggc gtgcctggct ggcgtcagca ttggcgaaca ccgacagcgc
     3901 gatccggtcg tcgcccgcgt ccgaagcgcg gagcaatatt tcgccgtccg ccggcgcttc
     3961 gtccatcacc acgatcgggc tgcccgcgta aaagtccgcg agcgcggcgc gcagcttgtc
     4021 gggcgaagcg gcgccgggca tcgcgttcag cggcaacggc acctcgacga tcatgccgcg
     4081 atgcgccggg atcacggcgg gcgagaaaag cggtccgatc gacaggccgc accgcgcctg
     4141 catttcgggc acatgcttgt ggccgagcgt cagcgcatag ccgcgccatg cgatgtcgga
     4201 atcctgctcg aaccgctcga tcagcgcctt gccgccgccg gaatagccgc tgaccgcgtg
     4261 gcagatatag ggccagtcgg cgggaagcag gcccgcgcgc accagcggag caacaagcgc
     4321 gatgaagccg gtcgaatagc agccggggtt cgacacgcgc gtggcggccg ccacggcgtc
     4381 atggccgctg acttcgggaa agccatagac ccagcccggc gcaacgcgat gcgcggtcga
     4441 ggcgtcgatg acgcgcgtgc gatcattgcc gatcatcgcc accgcttcgc gcgccgcgtc
     4501 gtcgggcagg cagaggatga cgaaatcggc atcgttcagc gcctcgcgcc gcgccgcggc
     4561 atccttgcgg cgcgcctcgc ccagcgtgat cagcgaaaac tcgctccgcc cggcgaggcg
     4621 ttcggcgatt tcgaggcccg tcgttcccgc cgcgccgtcg atgaaaaccg tctgtgtcat
     4681 gatgattatc gcagtctttt atatccgctc accggcggat catgatcctt tgcagccgaa
     4741 cgcgcgatga cgtcggccag cggttcggcc tttaccgcca tccatcgtcg gctggcgtgg
     4801 atgatattct tttcgtcggc catgatgccg acatggccgg gaaagaagac gaggtcgccg
     4861 cgtgcgagcg ccgccggatc gacatccttg tcggcaccga gcgcggcaag ctggaggtcg
     4921 ctgtcgcgcg gaatctggat gcccgccgcg ccccacacga gctggacgag gcccgagcag
     4981 tcgatcccct tcgcggtgcg tccgccccaa agatagggcg tgccgatcat ctgctccgcc
     5041 agttcggcgg ggtcgctgtc cccggtggtc acctcggcga gcgccgcgag cggcagatag
     5101 ccgtgcgaag tcgcgagcca ttcgccctcg acctcgccca tcaccagcgc cccgcgcggc
     5161 ag
//
LOCUS       DATMSU010000130         7179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_21022, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000130 DATMSU010000000
VERSION     DATMSU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21022"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..1322
                     /locus_tag="VN034_03485"
     CDS             561..1322
                     /locus_tag="VN034_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HWV59691.1"
                     /translation="MGEFLPESGEAAEILVVSLESAEARRHAFAARAADTSLAWRFFD
                     ACTGPAAGMTVDEAAIRRNKGRAMSKGEIGCYASHFSIWQDMVRRGVRQAIVLEDDTV
                     VDWAYLEPLARTDLHAEGIDYLRLYAKRPTWQRVVKRDFLQHSRAVVELVGLAYGTQG
                     YAITLAGARRLVEHCRTVRRPIDDEMDRSWAHGLPNLALYPAPILEAAIVSDIGNSRF
                     VPKNDPLYHSARQKLWRHIERARMRMLKARRLLER"
     gene            1312..2793
                     /locus_tag="VN034_03490"
     CDS             1312..2793
                     /locus_tag="VN034_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HWV59692.1"
                     /translation="MSADLPTRFDAGLRARSRSSIGWTVTRFLSDQIFSFVVFVILAR
                     LLTQADIGAFAVMAVTAEAFRIIATAGLVQTIARKKDITPAFLDTIYRSQQAFSFLSA
                     LLIMALAQPIANWMGAPDIALPLAVMSLTLPISSFGATHMALRLREFGHRTTALRLVV
                     GGLIGGTAAVVAAFAGLGLWSLVIQRLVTEIVGLILSRASYDWKPGWRFDWAILRGNL
                     MLNASLIYVQLAFLATVRVQEMAIGAGIGLAAVGIYRTAWRTVELIGRGAIQPFTQVA
                     VQTLARVKDDPAELRRAYRWMISRASALSFPALVGFGALAPIAIPTVFGAKWAEAGQL
                     AQIFAFMAVPFTLNFFASPSLSVLGASRSLISLSTTQLVLGVALTLAALPFGLFAVAV
                     SYVGRAYLTLPMQIWLLRRASGIRPIDSLRAVGPPLVASSLMGVALALAMRLLDGHLA
                     GWQALLLLTAVGTLFYGIALLAISGTWRGRLLSLSRQLRKSPI"
     gene            2790..3791
                     /locus_tag="VN034_03495"
     CDS             2790..3791
                     /locus_tag="VN034_03495"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HWV59693.1"
                     /translation="MTMKSADLLVGELADHFVRVLDAVLPEGKLALVDFPDHSNVGDS
                     AIWLGEMAYLRRARRLPAYYSAIADFDDEACRAAIGDGPILIHGGGNMGTLWPKHEAF
                     RLHLLRTHRGRPIVQMPQSIHYADPAAAAEMAEAIRDHGQFTLLVRDARSLAFAERHF
                     DCAIRLCPDAALMLGRQQRSPATVPVFALLRTDHERAVGGADALPAGVVADDWLEEDP
                     AQKRRLRLSLKLGRLFNRDPMGQRAARQQRLAEWRLQRGLAMLSTGELVVTDRLHAHI
                     LSLLLDIPHVLLDNSYGKVAGFADQWTGDYGGLMRATGRAEAFETALASVAPQIVDG"
     gene            complement(3798..5186)
                     /locus_tag="VN034_03500"
     CDS             complement(3798..5186)
                     /locus_tag="VN034_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HWV59694.1"
                     /translation="MTKIDLALDGAPVRRPLAKVARRDAKIWLCLALMGLDALALMAG
                     FAMTLAIEAPRSISDGLWTAMFGALLVHAAIAFQNQAYNPRCLTRPLKSCRQTLISFA
                     TTLLVFLLAAFSLKVTAQVPRLALGMGMVTALTLLVAQRLLICHIVSRVFGEMTAELV
                     IVDGANLPPSYLKRDIIDARASGLRTDLDDPYMLDRLGTVLQDYDRVTIACAPERKVE
                     WARALKGVNILGEIVMPEIAELGPLTTRQAGGVTTLVVSWGPLNRANRVKKRALDLAV
                     TIPALLALAPFLMLVAIAIKLDSPGPVFFRQDRMGRGNRIFRILKFRSMRVEATDANG
                     ARSASRDDDRITRVGKFIRATSIDELPQLINVLSGEMSLVGPRPHALGSTAGNELFWR
                     VDRQYWHRHALKPGITGLAQIRGFRGATNTKRDIVNRLGADLEYQQGWSLLRDVSILV
                     RTAQVLIHRNAY"
     gene            complement(5321..5941)
                     /locus_tag="VN034_03505"
     CDS             complement(5321..5941)
                     /locus_tag="VN034_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWV59695.1"
                     /translation="MADQCCAGKSGKTALNDPKWRRVLWLALLINAVMFGVEIVAGVA
                     ADSRALQADALDFLGDSANYAISLGVAGMALAWRARAALLKAATMLAFGLWVFGSAVW
                     GFVAGSAPHAETMGAIGALALAANVAVALMLYRYRTGDANMRSVWICSRNDAIGNIAV
                     MGAALGVFGTGRSWPDLAVAAIMAGLAIWGSLEVFRQARGELVAGT"
     gene            6011..6421
                     /locus_tag="VN034_03510"
     CDS             6011..6421
                     /locus_tag="VN034_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV59696.1"
                     /translation="MKIGELSRATGTNIETIRYYERIGLLPAPDRTAANYRSYGPAHR
                     SRLSFVRHSRELGFTIEEIRSLLDLSDDPARDCGEADRIATAHLEQVEAKIAQLALLR
                     DELARIVGRCRGGVAADCRVIEALGNHSRCENPH"
     gene            6604..>7179
                     /locus_tag="VN034_03515"
     CDS             6604..>7179
                     /locus_tag="VN034_03515"
                     /inference="COORDINATES: protein motif:HMM:NF033157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWFGD domain-containing protein"
                     /protein_id="HWV59697.1"
                     /translation="MERQTNKDRGSRDENSDRDTQSGSLYDWESNRDDGGYAYGGYPE
                     AGFGPRETRRYGDYGRSGRERTERGHYSADRPRDYAGNDWNRTYGARRYWDDDQRYGE
                     RDDKDRYRGSRDRDWRDSEDRQSAWAYMGYADPYGWPTYIPYGAADFGRSTYDRGGYS
                     PRGRHERGFFERAGDEVLSWFGDEEAQRRRER"
BASE COUNT         1163 a         2456 c         2324 g         1236 t
ORIGIN      
        1 cgcgtcgaga cagcggccga gcacggcttc gccgttccac acgggtatga cgactgcgac
       61 ttcaaacatg gattcgcctg tctaggatcc gtgctcgatt agggcgccgt cgaggcgtcc
      121 aaatggcgaa catatcgggc cgaaagacgc gccgcgaggg ctggttgccc ttgcaagcgg
      181 ctttcggttc gagatggaag ccatttggac gtcccgaagg gatttgatca aaatggccca
      241 ttgcttcgtc aggaagtctc gaaatattga catatgtctt cgccttcttt cctcgcactg
      301 ggccattttg tttcaaacct cgacggcgcc ctaatcgagc acggatcctg gtgcccccgc
      361 acgggtccaa tcggcccccc gccccgcccg ggtcaaccat atgctgcacc tgcgaagatg
      421 agccgccccg ttgaccggac gaccggagcc ggggcgcctt cgctctcaaa taattgcccg
      481 ctaccaagga tcgcgtcggg ccggtcgccc gttctgcacc gcacagactg gcggcggcgg
      541 cgaataggag acgtatatcc gtgggtgagt tcctgcccga atcgggcgaa gccgccgaaa
      601 tattggtggt gtcgctcgaa tccgccgagg ctcggcgcca cgccttcgcc gcgcgcgccg
      661 cagacacgtc gctcgcctgg cgctttttcg acgcctgcac cggccccgcg gcggggatga
      721 cggtcgacga agccgcgatc cgccgcaaca aaggccgcgc gatgagcaag ggcgagatcg
      781 gctgctacgc gagccatttt tcgatctggc aggacatggt gcgtcgcggc gtgcgccagg
      841 cgatcgtcct cgaggacgat acggtggtcg actgggctta tcttgagccg ctcgcgcgca
      901 ccgaccttca cgccgagggc atcgattatc tgcggctcta tgccaagcgg ccgacgtggc
      961 agcgcgtggt caagcgcgac ttcctccagc attcgcgcgc ggtcgtcgag ctggtcgggc
     1021 tcgcttacgg tacgcagggt tatgcgatca cgctcgcggg ggcgcggcgg cttgtcgagc
     1081 attgccgcac cgtccgccgc ccgatcgacg acgagatgga ccggtcgtgg gcgcacggcc
     1141 tgcccaatct cgcgctctac ccggcgccga tcctcgaggc ggcgatcgtc tcggacatcg
     1201 gcaattcgcg cttcgttccc aagaacgacc cgctctatca ttcggcaagg caaaagcttt
     1261 ggcgccatat cgagcgcgcg cgcatgcgca tgctgaaagc gaggcgcctg cttgagcgct
     1321 gacctcccca cccgcttcga cgcggggtta cgcgcacgca gccgcagttc gatcggctgg
     1381 acggtcacgc gtttcctgtc cgatcagata ttctccttcg tcgtcttcgt catcctcgcg
     1441 cgcctgctga cccaagcgga catcggcgcc ttcgcggtga tggcggtgac cgcggaggcg
     1501 ttccgcatca tcgcgaccgc cggcctcgta cagacgatcg cgcgcaagaa ggacatcacg
     1561 ccggccttcc tcgacaccat ctatcgctcg cagcaggcct tttccttcct ctccgccttg
     1621 ctgatcatgg cgctggccca acccatcgcc aattggatgg gcgcgcccga catcgcgctg
     1681 ccgctcgcgg tgatgagcct gacgctgccg atctcttcgt tcggcgcgac gcatatggcg
     1741 ctgcgcctgc gcgagttcgg gcaccgcacg accgcgctgc gcttagtcgt cggcgggctg
     1801 atcggcggga ccgcggccgt ggtcgccgcc ttcgcggggc tcggcttgtg gagcctcgtg
     1861 atccagcgcc tcgtcaccga gatcgtcggg ctcatattgt cgcgcgcctc ctatgactgg
     1921 aaacccggct ggcggttcga ctgggccata ttgcgcggca atctgatgct caacgccagc
     1981 ctgatctatg tccagctcgc cttcctcgcg acggtccgcg tgcaggagat ggcgatcggc
     2041 gccgggatcg ggctcgccgc cgtcggcatc tatcgcaccg cctggcgcac cgtcgagctg
     2101 atcggccgcg gcgcgatcca gcccttcacc caggtcgcgg tgcagacgct cgcgcgcgtc
     2161 aaggacgacc ccgccgaact gcggcgtgcc tatcgctgga tgatttcgcg cgcctcggcg
     2221 ctgtcctttc cggcgctggt cggtttcggc gcgctcgccc cgatcgcgat cccgaccgtg
     2281 ttcggcgcca aatgggccga ggcggggcag ctcgcgcaga ttttcgcctt catggcggtg
     2341 cccttcacgc tcaacttctt cgcttcgccc tcgctcagcg tgctcggcgc gtcgcgcagc
     2401 ctgatctcgc tgtcgacgac ccagctcgtg ctcggcgtcg cgctgacgct cgcggcgttg
     2461 cccttcggac tcttcgcggt cgccgtttct tacgtggggc gcgcctatct caccctgccg
     2521 atgcagatct ggctgctccg ccgcgcgtcg ggcatccggc cgatcgacag cctgcgcgcg
     2581 gtcggaccgc cgctggtcgc ctcctcgttg atgggcgtcg cgctcgcgtt ggcgatgcgg
     2641 ctgctcgatg ggcatctcgc cggctggcag gccttgctgc tgctcaccgc ggtcgggacg
     2701 ctcttttacg gtatcgcgct gctcgccatc tccgggacgt ggcgcggccg tctcctctcc
     2761 ctctccagac agctcagaaa gagcccgata tgaccatgaa aagcgccgac ctgctcgtcg
     2821 gcgaacttgc cgaccatttt gtccgcgtgc tcgatgccgt gctgccggaa ggtaagctgg
     2881 ccctcgtcga ctttcccgac cattccaatg tgggagactc ggcgatctgg ctcggcgaaa
     2941 tggcctatct gcgcagggcg cgccgcctcc ccgcctatta ttcggcgatc gccgatttcg
     3001 acgacgaagc ctgccgcgcg gcgatcggcg acgggccgat cctgatccac ggcggcggca
     3061 acatggggac gttatggccc aagcatgaag ccttccggct gcacctgctc cgcacgcacc
     3121 gcggccgccc gatcgtgcag atgccgcagt cgatccatta tgccgacccc gccgccgcgg
     3181 ccgagatggc cgaagcgatc cgcgatcacg gccagttcac cctgctcgtc cgcgatgccc
     3241 gctcgctcgc ctttgccgag cggcatttcg actgcgctat ccggctctgt cccgatgccg
     3301 cgctgatgct cggccgccag cagcggagcc ccgcgaccgt gccggtcttc gcgctgctcc
     3361 gcaccgatca tgagcgggcg gttggcggcg ccgatgcgct ccccgccggg gtggtcgccg
     3421 acgactggct cgaagaagac cccgcccaaa aacgccgctt gcgtctgtcg ctcaagctcg
     3481 gccgcctctt caatcgcgat ccgatggggc agcgcgccgc tcggcaacag cgcctcgccg
     3541 aatggcggct acagcgcggt ctcgcgatgc tttctaccgg cgagctggtc gtcaccgacc
     3601 ggctccacgc gcacatcctc tcgctgttgc tcgacatccc gcacgtgctg ctcgacaaca
     3661 gctatggcaa ggtcgcgggc ttcgccgatc aatggacggg cgattatggc ggattgatgc
     3721 gcgcgaccgg ccgcgccgag gctttcgaaa ccgcgctcgc cagcgtcgcg ccgcagatcg
     3781 tcgacggctg agccgcctca ataggcgttg cggtggatca gcacctgcgc cgtgcgcacc
     3841 aggatcgaaa cgtcgcggag tagcgaccag ccctgctgat attcaaggtc ggcgcccagc
     3901 cggttgacga tatcgcgctt ggtgttggtc gcgccgcgaa agccgcggat ctgcgcgagc
     3961 ccggtgatgc cgggcttgag cgcgtgacga tgccaatatt gccggtcgac gcgccagaac
     4021 agttcgttcc cggcggtcga gcccagcgcg tgcgggcgcg gcccgaccag gctcatctcg
     4081 cccgagagca cgttgatcag ctgcggcagt tcgtcgatgc tcgtcgcgcg gatgaatttg
     4141 ccgacgcgcg tgatccggtc gtcgtcgcgg ctcgccgagc gggcgccgtt cgcatcggtc
     4201 gcctcgacgc gcatgctgcg gaacttgagg atccggaaaa tgcggttgcc gcgccccatc
     4261 cggtcctgcc ggaaaaacac cggccccggc gaatcgagct tgatcgcgat cgcgaccagc
     4321 atcagaaagg gcgccagcgc gagcagcgcc gggatcgtca ccgcgaggtc gagcgcgcgc
     4381 ttcttgaccc ggttggcgcg gttgagcggg ccccacgaca ccaccagcgt cgtcaccccg
     4441 cccgcttggc gcgtggtcag cggccccagt tcggcgatct cgggcatcac gatttcgccg
     4501 aggatgttca cgcccttcag cgcgcgggcc cattcgacct tgcgttcggg cgcgcaagcg
     4561 atcgtcaccc ggtcgtaatc ctggagcact gtaccgagcc ggtcgagcat atagggatcg
     4621 tcgagatcgg tgcggagccc cgacgcgcgc gcgtcgatga tatcgcgctt cagataggat
     4681 ggcggcaggt ttgcgccgtc gacgatcacc agctcggcgg tcatttcgcc gaaaacacgg
     4741 ctgacgatat gacagatgag gagcctctgg gcgacgagca gcgtcagcgc ggtcaccatg
     4801 cccataccga gcgcgaggcg cggcacctgc gcggtcactt tcagcgagaa ggcggcgagc
     4861 aggaagacga gcagggtggt cgcgaaagag atcagcgtct ggcggcagct cttgagcggc
     4921 ctggtcaggc agcgcgggtt ataggcctgg ttctgaaacg cgatcgccgc gtgaacgagc
     4981 agcgcgccga acatcgccgt ccacaatccg tccgagatgc tgcgcggcgc ctcgatcgcc
     5041 agcgtcatcg cgaagcccgc catcagcgcc aacgcgtcga gccccatcag ggcaaggcag
     5101 agccaaatct tggcgtcgcg gcgtgcgacc ttcgcgagcg gtcttcggac gggcgcccca
     5161 tcgagcgcca gatcgatctt cgtcatggga tgggggactc cctaggttca cgcaatcttg
     5221 ttgcgatgca acatgtccgg ggaagccaga aatacaagcc gccagcgcgg gcttgtgccg
     5281 gatacaggat gatccgtgcg gaggacgtcc ggccgggggt tcaggtgccc gccacgagtt
     5341 cgccccgcgc ctgccggaaa acctcgaggc tgccccagat cgcaaggccc gccatgattg
     5401 ccgcgacggc gaggtcgggc cagctgcgtc cggtgccgaa cacgccgagc gcggcgccca
     5461 tcaccgcaat attgccgatc gcgtcgttgc gcgagcagat ccagaccgag cgcatgttcg
     5521 cgtcgcccgt ccggtagcga tagagcatca gtgcgaccgc gacattggcg gcgagcgcca
     5581 gcgcgccgat cgctcccatc gtctcggcat gcggcgccga cccggcgacg aagccccaca
     5641 ccgccgatcc gaacacccaa agcccgaagg cgagcatcgt tgccgccttc agcaaggcgg
     5701 cgcgggcgcg ccaggcgagc gccatgcccg cgacgccgag gctgatcgca taattggcgc
     5761 tgtcgcccag aaaatcgagc gcgtcggcct gcagcgcgcg cgaatccgcc gcgacccccg
     5821 cgacgatctc gacgccgaac atgacggcgt tgatcagcaa cgcgagccac agcacccgcc
     5881 gccatttggg atcattgagc gcggtcttgc cgcttttgcc ggcacagcat tgatcggcca
     5941 ttgccgaaat ctcctgttat cttcgagtcg gcatcctata tgcactctgt agcaactaca
     6001 gggtcaagcc atgaagatcg gcgagctgtc gcgtgccacc ggcacaaata tcgaaaccat
     6061 ccgctattat gagcgcatcg gcctgcttcc cgcgcccgat cgcaccgcgg ccaattatcg
     6121 cagctatggc ccggcgcacc ggtcgcggct gagcttcgtg cgccattcgc gcgagctggg
     6181 cttcacgatc gaggaaatcc ggtcgcttct cgacctatcg gacgatccgg cgcgcgactg
     6241 cggcgaggcc gaccggatcg cgaccgcgca tctggagcag gtcgaggcga agatcgcgca
     6301 gctggccctg ctccgcgatg agctcgcgcg catcgtcggg cgctgccgcg gcggggtcgc
     6361 cgccgattgt cgggtgatcg aagcgctcgg caaccacagc cgctgcgaaa atccgcactg
     6421 aagggctcgt tgccgaacga aacagccgcg accaacatgg atcaaggtcc aacgtctatt
     6481 tccgctcgtt ttgtcgaatg ggcaggaacc ccgggcgccg cgcacgcgtc agtggggcag
     6541 gttgggggcg tcggcccccg cacgcctgcc aaccgctccc ccagcgaaac gaggagacgg
     6601 aaagtggaaa ggcagacgaa caaagatcgc ggcagccgtg acgaaaacag cgatcgcgat
     6661 acgcaatcgg gttcgctcta tgattgggaa tcgaaccgcg acgacggcgg ttacgcttat
     6721 ggcggatatc ccgaagccgg attcggtccg cgcgagacgc ggcgctatgg cgactatggc
     6781 cgcagcgggc gcgaacgcac cgaacgcggc cattacagcg cggatcgtcc gcgcgactat
     6841 gccggaaacg actggaatcg cacctatggc gcgcggcgct attgggacga tgatcagcga
     6901 tatggcgagc gcgatgacaa ggaccgctac cgcggttcgc gtgatcgcga ttggcgcgac
     6961 agtgaggatc gccaatcggc ctgggcttat atgggatatg ccgaccccta tggctggccg
     7021 acctatatcc cctatggcgc cgccgatttc ggccgatcga cctatgaccg cggcggctat
     7081 tcgccgcgcg gacgtcacga gcgcggcttc ttcgagcgcg cgggcgacga ggtgctgtcg
     7141 tggttcggcg acgaggaagc gcagcgccgc cgcgagcgc
//
LOCUS       DATMSU010000131         1966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_97410, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000131 DATMSU010000000
VERSION     DATMSU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97410"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VN034_03520"
     CDS             <1..1251
                     /locus_tag="VN034_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWV59698.1"
                     /translation="DHQALLGPDYQLADYEPQNDILDVWFDSGSTHVFTVEARYGEGV
                     RADLYLEGSDQHRGWFQSSLLESCGTRGQAPYKAVLTHGFTMDAKGFKMSKSLGNTIS
                     PIDLMRDYGADILRLWALSVDFTEDHRIGKEILTGVADQYRKLRNTFRYLLGALDGFG
                     EEERITDIAAMPELERYMLNQLAHLHLHLREAIDDFDFNTYTRLLADFCNEDLSAFYF
                     DIRKDVLYCDLGPAAPLGTSTRRAYRSVLDILFHALIRYAAPVLVFTSEEVWGTRYPD
                     EGSVHLLELQELDVGQWIDGNLASRWDRLREVRSQVTEAIEPYRREKTVRSSLEAEVS
                     VRTKDAALASILVDVGTEELAELLIVSAANTTGFTDDTVADITMVDVTRTENHKCGRC
                     WRHLPEVKTDGSLCNRCDTLLTSA"
     gene            1248..1769
                     /gene="lspA"
                     /locus_tag="VN034_03525"
     CDS             1248..1769
                     /gene="lspA"
                     /locus_tag="VN034_03525"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWV59699.1"
                     /translation="MSSTRSPYLRFGLIFAAAAFILDQIVKWVVTVPLSLEAKGQIEL
                     ASIFNLTWAENCGISLSMFASCTDTTRWTLVAVTGIVAAAVAFWMTREQAKGDVIALA
                     MILGGALGNIVDRVRFGYVVDFADLHFGDFRPFMIFNVADACITLGVLLLVARALLLG
                     EKAPAADARPSAE"
     gene            1795..>1966
                     /locus_tag="VN034_03530"
     CDS             1795..>1966
                     /locus_tag="VN034_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HWV59700.1"
                     /translation="MNRTTAILAASIFATTLSACGSNSLFSRDRPDEMMVSRQAPLVV
                     PPDFALTPPAPSR"
BASE COUNT          348 a          663 c          583 g          372 t
ORIGIN      
        1 gaccaccagg cgctactcgg ccccgactac cagctcgcgg attacgagcc gcagaatgac
       61 attctcgacg tgtggtttga ttcgggctcg acccacgtct tcaccgtcga ggcgcgatac
      121 ggcgagggcg tgcgcgccga cctctatctc gaagggtcgg accagcatcg cggctggttc
      181 cagtcgtcgc tgctcgaaag ctgcggcacg cgcgggcagg cgccgtacaa ggcggtgctg
      241 acccacggct tcacgatgga cgccaagggc ttcaaaatgt cgaagtcctt gggcaacacg
      301 atcagcccca ttgacctgat gcgcgactat ggcgccgaca tcctccgcct gtgggcgctc
      361 tcggtcgact tcaccgagga ccaccgcatc ggcaaggaga tcctgaccgg ggtcgccgac
      421 cagtatcgca agctgcgcaa taccttccgc tacctgctcg gcgcgctcga cgggttcggc
      481 gaagaggagc gcatcaccga catcgcggcg atgcccgagc tcgaacgcta tatgctgaat
      541 cagctcgcgc acctccacct gcatctgcgc gaagccatcg atgatttcga cttcaacacc
      601 tacacgcgct tgctcgccga tttctgcaac gaggatctgt cggccttcta tttcgacatc
      661 cgcaaggacg tcctctattg tgacctcggc cccgccgcgc cgctcggcac cagcacgcgc
      721 cgcgcgtacc gcagcgttct cgatatcctc ttccacgcgc tgatccgcta cgccgcgccg
      781 gtgctggtct tcaccagtga agaggtgtgg ggcacacgct atccagacga gggcagcgtg
      841 cacctgcttg agttgcagga actcgacgtc gggcaatgga tcgacggcaa ccttgcctcc
      901 cgttgggatc gtttgcgcga ggttcgctcg caagtcaccg aggcaatcga gccttatcgt
      961 cgagaaaaga cggttcgctc gtcgctggaa gccgaagtgt cggttcgcac caaggacgcc
     1021 gcattggcca gcatccttgt tgatgttggc acggaagaac tcgccgaact gctcatcgta
     1081 tcggcggcaa acacgaccgg gttcactgac gatacagtcg ccgacatcac gatggtcgac
     1141 gtcacccgca ccgaaaacca caaatgcggc cgctgctggc gccacctgcc cgaggtcaag
     1201 accgacggca gcttgtgcaa tcgatgcgac acgctattga cctcggcatg agcagcacac
     1261 gttcgcccta tctgcgcttc ggcctgattt tcgccgccgc cgccttcatc ctcgaccaga
     1321 tcgtcaaatg ggtcgtcacc gtccccttat cgctcgaagc caaggggcag atcgagcttg
     1381 cgagcatctt caacctgacc tgggcggaga attgcggcat ttcgctgtcg atgttcgcga
     1441 gctgcaccga cacgacgcgc tggacgctcg tcgcggtgac ggggatcgtc gccgccgcgg
     1501 tcgccttctg gatgacgcgc gaacaggcga agggcgacgt gatcgcgctc gcgatgatcc
     1561 tcggcggcgc gctcggcaat atcgtcgacc gcgtgcgctt cggctatgtc gtcgatttcg
     1621 ccgacctgca tttcggcgat ttccgcccct tcatgatctt caacgtcgcc gatgcgtgca
     1681 tcacgctcgg cgtgcttttg ctggttgcgc gcgcgctgct cttgggcgaa aaggccccag
     1741 ccgcggacgc caggccgtcc gccgagtaaa cggggcgcat aggagttttt tgacgtgaac
     1801 cggaccaccg ccatcctggc agcctcgata tttgccacca ccctgtcggc ctgcgggtcg
     1861 aacagcctgt tcagccgcga ccgtccagac gagatgatgg tgtcgcgtca ggcgccgctc
     1921 gtcgtgccgc ccgatttcgc gctgaccccg cccgcgccca gcaggc
//
LOCUS       DATMSU010000132         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_97415, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000132 DATMSU010000000
VERSION     DATMSU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97415"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VN034_03535"
     CDS             <1..260
                     /locus_tag="VN034_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWV59701.1"
                     /translation="IVDEGGIAAILQLRLARTPSPDIVAAVTGMKEVARCYSLSGEID
                     LLVEIEAPDAAALNAARDRIALIAGVEDTTTALILKRDKDR"
     gene            complement(269..637)
                     /locus_tag="VN034_03540"
     CDS             complement(269..637)
                     /locus_tag="VN034_03540"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWV59702.1"
                     /translation="MPNRAINSTGVSYAQAHLVEDPKRWLFVSGQIPVDEKDAVPAGF
                     EDQCRLVWRNIEKQLLAGGMTLQNLVKVTVFLSDRRYREANYKVRHEVLGGHSPALTI
                     IIAGIYDEAWLLEIEAIAAA"
     gene            872..1198
                     /locus_tag="VN034_03545"
     CDS             872..1198
                     /locus_tag="VN034_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWV59703.1"
                     /translation="MTQASPDLLFKALADPSRRALFERLCLEGEQTVGALTAGAGISQ
                     PAVSKHLGVLKQAGLVLDRHEGRQTHYSAQQRALAPLVDWTGRMNRFWEARFDDLEDL
                     LKRMDQ"
     gene            1195..1617
                     /locus_tag="VN034_03550"
     CDS             1195..1617
                     /locus_tag="VN034_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWV59704.1"
                     /translation="MTDTDVRTVTVEREIPHPPEKIWRALTTQHLIEEWLMKNDFGLY
                     LGHRFQLRGDWGHVDCEILDVEPERSLSYTWNYAHDDPAYRLDSKVTFTLEPTPAGTL
                     LRMEQVGFRPDQKQAFGGAKGGWKIHLENLEKVVASLD"
     gene            1639..1854
                     /locus_tag="VN034_03555"
     CDS             1639..1854
                     /locus_tag="VN034_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59705.1"
                     /translation="MNWSLWIRQFHRWVSIIFTAIVLAIFVMLGVGQEPAQWVYYLPL
                     PPLFLLMFSGLYMFFRPYFRKRGGAAG"
     gene            complement(1859..>2095)
                     /locus_tag="VN034_03560"
     CDS             complement(1859..>2095)
                     /locus_tag="VN034_03560"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123423.1"
                     /note="Catalyzes the reversible hydration of unsaturated
                     fatty acyl-CoA to beta-hydroxyacyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWV59706.1"
                     /translation="SAPVSVALTRHMLWRMLGAPHPMSAHRWDSRAIFARGRSADAAE
                     GVSSFLEKRPANFTVSVANDYPWFAEFEDTPPYG"
BASE COUNT          382 a          665 c          689 g          359 t
ORIGIN      
        1 gcatcgtcga cgagggcggg atcgccgcga tcctgcaact gcggctcgcg cgcacgccct
       61 cgcccgacat cgtcgcggcg gtgacgggca tgaaggaggt cgcgcgctgc tattcgctga
      121 gcggcgagat cgacctgctc gtcgagatcg aggcgcccga cgcggccgcg ctcaacgccg
      181 cgcgcgaccg gatcgcgctg atcgcggggg tcgaggacac aacgaccgcg ctgatcctga
      241 aacgcgacaa ggatcgctga cgggatcgct aggcggcggc gatcgcctcg atctcgagca
      301 gccacgcctc gtcgtagatg ccggcaatga tgatcgtcag cgccggcgaa tggccgccga
      361 gcacttcgtg ccggaccttg tagttcgcct cgcgatagcg gcggtccgac aggaagaccg
      421 tgaccttgac gagattctgc agggtcatcc cgccggccag aagctgtttt tcgatattgc
      481 gccagacgag ccggcattga tcctcgaagc cggcgggcac cgcgtccttc tcatcgaccg
      541 gaatctggcc gctgacgaac agccagcgct tcgggtcttc gacaagatgc gcttgtgcgt
      601 aggagacgcc ggtggagttg atcgcgcgat tgggcatgcg gggcctcgtc attccgatgg
      661 aggggacggc gatcccggcc agcaccccgg cgcagaccga gcgacgcgag aggtgaaggc
      721 gaggggcgtc cgatgggttc accgcagcat ctagcgcgaa ggcgatggcg ggctccagcg
      781 tccgatgggc ggggcgcccg tcataaaggc ggcgcttgac actcataact atatggttat
      841 gagaaaacgc atagccagtg agttatgaat tatgacgcaa gcctcgcccg accttctctt
      901 caaggcgctc gccgacccca gccggcgtgc gctcttcgaa cggctctgcc tcgaaggcga
      961 gcagaccgtc ggcgcgctca ccgcgggcgc ggggatttcg cagcccgcgg tgtcgaaaca
     1021 tcttggcgtg ctcaagcagg cggggctcgt gctcgaccgg catgagggcc ggcagacgca
     1081 ttacagcgcg cagcaacgcg cgctcgcgcc gctcgtcgac tggaccgggc gcatgaaccg
     1141 cttctgggaa gcgcggttcg acgatctcga agatttgctc aaaaggatgg atcaatgacc
     1201 gataccgacg tgcgcaccgt aactgtcgag cgcgaaattc cgcacccgcc cgaaaaaatc
     1261 tggcgcgcgc tgacgacgca gcatctgatc gaggaatggc tgatgaagaa tgacttcggc
     1321 ctctacctcg gccaccgttt ccagctgcgc ggcgactggg gccatgtcga ttgcgagatc
     1381 ctcgacgtcg aacccgaacg cagcctgtcc tatacgtgga attatgcgca tgacgatccg
     1441 gcctaccggc tcgacagcaa agtgaccttc acgctcgaac cgacgcccgc cggaacgctg
     1501 ctgcgtatgg aacaggtcgg tttccgtccc gaccagaagc aggccttcgg tggcgccaag
     1561 ggcggctgga agattcacct cgaaaatctc gagaaggtgg tcgcgagtct cgattgaact
     1621 tcaaacaggg agaagaacat gaactggagt ctatggatcc ggcagtttca ccgctgggta
     1681 tcgattatct tcaccgcgat cgtgctcgcc attttcgtga tgctcggcgt ggggcaggag
     1741 ccggcccagt gggtctatta cctgccgctg ccgccgctct tcctgctgat gttctcgggc
     1801 ctctacatgt tcttccggcc ctatttccgg aaacgcggcg gcgcggcggg ctgaccgctc
     1861 aaccgtacgg cggcgtatcc tcgaactcgg cgaaccaggg atagtcgttc gcgacgctga
     1921 cggtgaagtt cgccggccgc ttttcgagga agctcgacac gccctcggcg gcgtcggcgc
     1981 tgcgcccgcg cgcgaagatc gcgcggctgt cccagcggtg cgcgctcatc ggatgcggcg
     2041 cgccgagcat ccgccacagc atgtggcgcg tcagcgcgac cgacaccggc gcgct
//
LOCUS       DATMSU010000133         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_144210,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000133 DATMSU010000000
VERSION     DATMSU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144210"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VN034_03565"
     CDS             complement(<1..298)
                     /locus_tag="VN034_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWV59707.1"
                     /translation="MSRLILFNKPYGVLSQFTDRGMPEARATLSDYIDVPGVYPAGRL
                     DRDSEGLLILTDDGALQARISSPKHKTPKTYLAQVEGEPDAAAIEALRRGVTLSD"
     gene            complement(500..2332)
                     /gene="typA"
                     /locus_tag="VN034_03570"
     CDS             complement(500..2332)
                     /gene="typA"
                     /locus_tag="VN034_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWV59708.1"
                     /translation="MSLRNIAIIAHVDHGKTTLVDQLFRQSGTFRDNQRVEERAMDSN
                     DLEKERGITILAKCTSVEWGEGADTTRINIVDTPGHADFGGEVERILSMVDGVILLVD
                     AAEGPMPQTKFVTGKALALGLKPIVVVNKIDRSDARAAEVLDEVFELFLALEANDEQL
                     DFPILYASGRGGYASESPEAREGTLEPLFKTIVSHVPTPGLDEDAPFTFLATLLDRDN
                     FIGRILTGRVQSGSVKVNQPIHALDMDGKVIETGRASKLLAFRGLDRVPVDEAKAGDI
                     VAIAGLTNATVANTIADTSVSEPIAAQPIDPPTLSMRFAVNDSPMAGREGSKVTSRMI
                     RDRLLREAETNVAIRITESADKDSFDVAGRGELQLGVLIETMRREGFELGISRPKVLY
                     GEDEDGKRTEPYETVVIDVDDEHSGTVVEKMQIRKADLTDMRPSGGGKTRITFSGPSR
                     GLIGYHGEFLSDTRGTGIMNRLFEKYGPYKGVIEGRKNGVLISNGNGEAVAYALGPLE
                     ERGILFVSPGEALYEGMIIGENAKPDDLEVNPMKSKQLTNFRSTGKDDAIRLTPPKRM
                     TLEQAIAYIDDDEMVEVTPKNIRIRKALLDPHERKKASRKKEAA"
     gene            complement(2441..>2752)
                     /locus_tag="VN034_03575"
     CDS             complement(2441..>2752)
                     /locus_tag="VN034_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59709.1"
                     /translation="AAGARLMIDRADPQGVRLAIAAMRYQLGLVAERYAGPRFARDRT
                     ALLAGSRQLQAIGEIADPREARLVLDRWKRGFDAGLARRLRGAEVRSLYDRKRLDRAL
                     R"
BASE COUNT          418 a          864 c          947 g          523 t
ORIGIN      
        1 cgtcgctgag ggtgacgccg cggcgcagcg cctcgatcgc ggcggcgtcg ggctcgccct
       61 cgacctgcgc gagataggtt ttgggcgtct tgtgcttcgg cgacgaaatc ctcgcctgca
      121 acgcgccgtc gtcggtgagg atcagcaggc cttcactatc cctgtccagt cgccccgccg
      181 gatagacgcc ggggacgtcg atataatcgg acagtgtcgc gcgcgcctcg ggcatgccgc
      241 ggtcggtgaa ttgcgacagg acgccgtagg gcttgttgaa caggatcagg cggctcacgg
      301 cgtcgctcct cccatatgcc ctccgctcct ttttccctcg tcaccctgaa cttgtttcag
      361 ggtccatggc ctgccctttc cggcaacgca gcaccgaacg agagagtggg ccatggatgc
      421 tgaaacgagt tcagcatgac gaaaaccggg aggggcgcca aagaaaaacc ggcggccgat
      481 ggggccgccg gtccttgtct tacgccgctt ccttcttgcg cgacgccttc ttgcgctcgt
      541 gcgggtcgag cagcgccttg cggatgcgga tgttcttcgg ggtcacctcg accatctcgt
      601 cgtcgtcgat ataggcgatc gcctgttcga gcgtcatgcg cttcggcggg gtcaggcgga
      661 tcgcgtcgtc cttgcccgtc gaacggaagt tggtgagctg cttcgacttc atcgggttga
      721 cctcgaggtc gtcgggcttg gcattctcgc cgatgatcat cccctcatag agcgcctcgc
      781 cgggtgagac gaagaggatg ccgcgctctt cgagcgggcc caaggcatag gcgaccgctt
      841 cgccattgcc gttcgagatc aggacgccgt tcttgcggcc ctcgatcacg cccttgtagg
      901 ggccatattt ctcgaacagg cggttcatga tgccggtgcc gcgcgtgtcc gacaggaatt
      961 cgccgtgata gccgatcagg ccgcgcgacg ggccgctgaa ggtgatgcgc gtcttgccgc
     1021 cgcccgacgg gcgcatgtcg gtgaggtcgg ccttgcggat ctgcatcttc tcgacgaccg
     1081 tgccgctatg ctcgtcgtcg acgtcgatga cgacggtttc atagggttcg gtgcgcttgc
     1141 cgtcctcgtc ctcgccatag aggaccttgg ggcggctgat gccgagttcg aagccttcgc
     1201 ggcgcatcgt ttcgatgagc acgccgagct gaagctcgcc gcggcccgcg acgtcgaagc
     1261 tgtccttgtc ggcgctttcg gtgatgcgga tcgcaacatt ggtttcggct tcgcggagca
     1321 ggcggtcgcg gatcatgcgg ctcgtgacct tgctgccctc gcggcccgcc atcgggctgt
     1381 cgttgacggc aaagcgcatc gacagcgtcg gcgggtcgat cggctgcgcg gcgatcggtt
     1441 cgctgacgct cgtgtcggcg atcgtgttcg cgacggtcgc gttggtgagg cccgcgatcg
     1501 cgacgatgtc gcccgccttc gcctcgtcca ccggcacgcg gtcgagcccg cggaaggcga
     1561 gcagcttcga tgcgcggccg gtttcgatca ccttgccgtc catgtcgagc gcgtggatcg
     1621 gctggttgac cttgaccgaa cccgactgaa cgcggccggt caggatgcgg ccgatgaaat
     1681 tgtcgcggtc gagcagcgtc gcgaggaagg tgaagggcgc atcttcgtcg aggcccggag
     1741 tcgggacatg gctgacgatc gtcttgaaca gcggttcgag cgtgccctcg cgcgcttcgg
     1801 ggctttcgga agcatagccg ccgcggcccg aggcgtagag gatcgggaaa tcgagctgtt
     1861 cgtcattggc ttcgagcgcg aggaacagct cgaacacttc gtcgagcact tcggcggcgc
     1921 gcgcgtcgct gcggtcgatc ttgttgacga cgacgatcgg cttcaagccc agcgcgagcg
     1981 ccttgccggt gacgaacttg gtctgcggca tcgggccttc ggcggcgtcg acgagcagga
     2041 tgacgccgtc gaccatcgac aggatacgct cgacctcgcc gccgaagtcg gcgtgaccgg
     2101 gggtgtcgac gatgttgatg cgcgtcgtgt cggcgccttc gccccactcg accgaggtac
     2161 atttggcgag aatggtgatc ccgcgttcct tctccaagtc attggaatcc atggcccttt
     2221 cctcgacgcg ctggttgtcg cggaaggttc ccgactggcg gaacagctgg tcgacgagcg
     2281 tggttttgcc atgatcgacg tgggcaataa tggcgatatt gcgcagagac atgcggattt
     2341 gagccttgat ggaagggggc agcgcccgga gcgccgcgaa acgccgcgcc cctaacagaa
     2401 gtggtgctgc ggcgcaacat gattgccgag ggtgcccgaa ctagcgaagc gcgcggtcga
     2461 gccgcttcct gtcgtagagc gagcgcactt cggcgccgcg gaggcgccgc gccagaccgg
     2521 cgtcgaaacc gcgcttccaa cggtcgagga ccagccgagc ctcgcggggg tcggcgatct
     2581 cgccgatcgc ctgcaattgg cgcgaccccg cgagcaaggc ggtgcggtcg cgggcgaaac
     2641 gcggtccagc atagcgctcg gcgacgagtc ccagttgata gcgcatggcg gcgatcgcca
     2701 ggcgcacgcc ctgcggatcg gcgcggtcga tcatcagccg cgcgcccgcg gc
//
LOCUS       DATMSU010000134         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_49799, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000134 DATMSU010000000
VERSION     DATMSU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49799"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VN034_03580"
     CDS             <1..443
                     /locus_tag="VN034_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HWV59710.1"
                     /translation="RWLRPKLWGVPPPPQGTEPVTHLRNLTSPFWIGTLRHAELRCLI
                     PATAFAYWSGAGGARRQHWFSVPSQPIFAFAGVVRQGEDWPCFAMLTTEANRLVAHHQ
                     PKAMPVILHPEDHATWLSGEWRAAVALAAPFPSQSMAVGDAPPV"
     gene            532..861
                     /locus_tag="VN034_03585"
     CDS             532..861
                     /locus_tag="VN034_03585"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV59711.1"
                     /translation="MLGNRTIETTRTPKAGRGAERAPVTARARFREPGLNPFNVELFD
                     LSSTGFRMVTSFRPEIGKHIWVNLPGLQPLEAVVRRADGNNYGCEFVHPLHVSVAQHL
                     QAKLRGQ"
     gene            complement(866..1291)
                     /locus_tag="VN034_03590"
     CDS             complement(866..1291)
                     /locus_tag="VN034_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWV59712.1"
                     /translation="MSKQYWLMKSEPDAYAWDQLVHDGTGIWDGVRNHTAKLNMMAMK
                     KGDEAFFYHSNIGKECVGIMEITEESFPDPTADKGSPWVVVRVAPVRALAHPVTLAAI
                     KADPKLADMDLIRQSRLSVGRVTPAEWKHILKKSEKPEG"
     gene            1421..2350
                     /locus_tag="VN034_03595"
     CDS             1421..2350
                     /locus_tag="VN034_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HWV59713.1"
                     /translation="MTAAVPVISMSLPADQFAKDFGASFERFGFAMITDHGIDAALID
                     EAWTKAKAFFALPEEVKRAYHIAGGGGARGYTPFGTEIAKGAKEVDLKEFWHVGRDLP
                     AGHRLAAQQPNNVWPAEVEGFRTAYDKLFAEFDRVGARLLSGIARYLGLAPDFFDDTV
                     ADGNSVMRLLHYPPVEAPAKGIRAEAHEDINTITLLLGAEEAGLEILDKDGSWLPVSP
                     PPGAMAVNVGDMLQRLTNNRLPSTTHRVRNPDAGRASVARYSMPFFLHFRSDYPIETL
                     ESCIDASHPNLYPTPITADDYLQERLREIGLKK"
     gene            2350..3417
                     /locus_tag="VN034_03600"
     CDS             2350..3417
                     /locus_tag="VN034_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV59714.1"
                     /translation="MRTAAALFALMACLASLRPAFAEAPQTLPWSAPATTKVVTEDRR
                     FTSGDRELAGTLHLPVSAKPVAAVVVTHSASSPLGSASLYDHLETILPALGIAVLTYD
                     RRGSGRSGTQDAGGDFAVLADDAIAAARSLKGDPRIDPRRIGTWGLSQGGWISPLAAS
                     RSPDIAFVVAVSAPVVTADVQMIFSSTNHLKANGYSQADVDQMVAARKAVDDYMRGTV
                     SRAEAQAKLDGIKTKPWFKYLYMGETFRDREVSGWRKEIENDPLVNLKAVTVPTLILF
                     GADDAVVPVADSAERLKAIAPQMPNMEVHVIAGADHAMQMSQDLKTSLDPKHDGTERP
                     DSPEYFALLASWLAARGLVED"
BASE COUNT          604 a         1231 c         1150 g          628 t
ORIGIN      
        1 ggcgctggct gcgcccgaag ctgtggggcg ttccgccgcc gccgcaaggc accgagcccg
       61 tcacgcacct ccgtaacctg accagcccgt tctggatcgg caccttgcgc catgccgagc
      121 tgcgctgcct gatccccgcg accgccttcg cctattggtc gggagcgggc ggcgcgcggc
      181 ggcagcactg gttttcggtg ccgtcgcagc cgatcttcgc ctttgccggc gtggtgcggc
      241 agggcgagga ctggccgtgc ttcgcgatgc tgacgaccga ggcgaaccgg ctggtcgcgc
      301 atcatcagcc caaggcgatg ccggtgatcc tgcatcccga agatcatgcg acctggctaa
      361 gcggcgaatg gcgcgccgcg gtggcgctcg cggcgccgtt tcccagccag tcgatggcgg
      421 tcggcgacgc accaccggta tagctggact tcaccatatc gctttacctt gcggtaacca
      481 gcggcgccct aaatctgccg cgaccaagaa tgacatgcag ggaccgaatc cgtgttgggg
      541 aatcgcacca tcgaaaccac aaggacgccc aaggccgggc gcggcgccga acgcgcgccg
      601 gtgaccgcgc gcgcgcgttt ccgcgaaccg gggctcaacc ccttcaacgt cgaattgttc
      661 gacctgtcgt cgaccggctt tcgcatggtg acatcgttcc ggcctgagat cggcaagcat
      721 atctgggtca acctgccggg actgcagcct ctggaagccg tcgtgcgccg cgccgacggc
      781 aataattacg gctgcgagtt cgtgcatccg ctccatgtgt cggtcgccca gcatctgcag
      841 gcgaagctgc gcggccagta agcggtcagc cctcgggctt ttccgatttc ttcaatatat
      901 gtttccactc ggcgggcgtc acgcgcccga ccgacagccg cgactggcgg atcagatcca
      961 tatcggcgag cttcgggtcg gccttgatcg ccgcgagcgt caccggatgg gcgagcgcgc
     1021 gcaccggcgc gacgcgcacc acgacccagg gcgagccctt gtcggcggtc gggtcgggga
     1081 acgactcttc ggtgatctcc atgatcccga cgcattcctt gccgatattg ctgtgataga
     1141 agaaggcctc gtcgcccttc ttcatcgcca tcatattgag cttcgcggtg tggttgcgca
     1201 ccccgtccca gatgccggtg ccgtcgtgca cgagctggtc ccacgcatag gcgtcgggtt
     1261 cggatttcat cagccaatat tgcttgctca tggcgcgggc ttagccgagc cgcgcggcgg
     1321 acggaaccgc ctttttccct ttcacgcgca ggccgtagcg cctaaatccg gttcatgacg
     1381 cgcctgcggg cgccgcccct ttcagatgga cttcaatcgc atgaccgctg ccgttcccgt
     1441 catttccatg tcgcttcccg ccgaccagtt cgcgaaggat ttcggggcgt cgttcgaacg
     1501 cttcggtttc gcgatgatca ccgatcacgg cattgacgcc gcgctgatcg acgaagcctg
     1561 gacgaaggcg aaggcgttct tcgcgctgcc cgaggaggtc aagcgcgcct atcatatcgc
     1621 cggaggcggc ggcgcgcgcg gctatacccc gttcgggacc gagatcgcca agggcgcgaa
     1681 ggaagtcgac ctcaaggaat tctggcacgt cggccgcgac ctgcccgccg gccaccgcct
     1741 cgccgcgcag cagccgaaca atgtctggcc cgccgaggtc gagggtttcc gcaccgccta
     1801 tgacaagctg ttcgccgaat tcgaccgcgt cggcgcccgc ctcctgtcgg gaatcgcgcg
     1861 ctatctcggc ctcgcccccg acttcttcga cgacaccgtc gccgacggca acagcgtgat
     1921 gcgcctgctc cactacccgc cggtcgaagc gcccgcgaag ggcatccgcg ccgaagcgca
     1981 tgaggatatc aacacgatca cgctgttgct cggcgccgag gaggcagggc tcgaaattct
     2041 cgacaaggac ggcagctggc ttcccgtcag cccgcccccc ggcgcgatgg cggtcaatgt
     2101 cggcgacatg ctccagcggc tcaccaacaa ccgcctgccc tcgacgacgc accgcgtccg
     2161 caaccccgat gcgggccgcg cgagcgtcgc gcgctattcg atgcccttct tcctccactt
     2221 ccgctcggac tatccgatcg aaacgctcga aagctgcatc gacgcgtcgc atccgaacct
     2281 ctacccgacg ccgatcaccg ccgacgatta tctgcaggaa cggctgcgcg agatcgggct
     2341 caagaagtga tgcgcaccgc cgctgctttg ttcgcgctta tggcgtgcct cgcctcgctg
     2401 cgccccgcct tcgcggaagc gccgcagacc ctgccgtgga gtgcgcccgc cacgacgaag
     2461 gtggtgaccg aggatcgccg gttcacgagc ggcgatcgcg agctggcggg aacgctccac
     2521 ctgccggtca gcgccaagcc cgtcgcggcg gttgtggtga cgcacagcgc ctcgtcgccg
     2581 ctcggttcgg cgtcgctcta cgatcatctc gaaaccatcc tgccggcgct cggcattgcc
     2641 gtcctcactt acgaccggcg cggatcgggc cggtcgggca cgcaggatgc cgggggcgac
     2701 ttcgcggtcc tcgccgacga tgcgatcgcc gccgcgcgga gcctgaaggg cgatccgcgc
     2761 atcgatcccc ggcgcatcgg cacctggggg ctgagccagg gcggctggat ttcgccgctc
     2821 gccgcgtcgc gcagccccga catcgccttt gtcgtcgcgg tgtcggcacc agtggtgacc
     2881 gccgacgtcc agatgatctt ttcctcgacg aaccatctga aagccaatgg ttattcgcag
     2941 gccgacgtcg accagatggt cgcggcgcgc aaggcggtcg acgattatat gcgcggcacg
     3001 gtgagccgcg ccgaagcgca ggcgaagctc gacggaatca agaccaagcc gtggttcaaa
     3061 tatctctata tgggcgaaac cttccgcgac cgcgaagtat cgggctggcg caaggagatc
     3121 gaaaatgatc cgctggtcaa tctgaaggcg gtcaccgtgc cgacgctcat cctctttggc
     3181 gccgacgatg cggtcgtccc ggtcgccgat tcggccgagc gcctgaaagc catcgcgccg
     3241 cagatgccga atatggaggt gcatgtgatt gcgggcgccg atcatgcgat gcaaatgtcg
     3301 caagacctca agacatcgct cgaccccaag cacgacggca ccgaacggcc cgattcaccc
     3361 gaatatttcg cgctgctggc gagttggctt gccgcgcggg ggttggttga ggactgagag
     3421 atcggtcccg agaagggaaa gtccgttttg gggtggggag tggacgatac ccaacccctc
     3481 ctcttcagag gaggggcagt gagacttggt ccggcgcagc cgggccttta gtcgaacggg
     3541 gtggtggtgg cggcacgcgc acgcctagcc gagaccacca ccccgctgcg actaacgaac
     3601 aagttcgtaa gtc
//
LOCUS       DATMSU010000135         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_87940, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000135 DATMSU010000000
VERSION     DATMSU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87940"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VN034_03605"
     CDS             complement(<1..629)
                     /locus_tag="VN034_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWV59715.1"
                     /translation="MNRPLVITDCDEVLMHMVVPFAEWVDAEHGVIFRIEDASFANAL
                     KRKECGTPLEAAEVWPLLDGFFRREMTRQYPIAGALAAMAAIGAEADIVVLTNVGPEH
                     QQARIEQLALHDFHAPVIGSRGGKGEPVRRLIEQYQPSVAVFIDDLAGHHMSVAQEAP
                     DVWRLHLVGEPAIAGKIAPAKHAHARIDDWTAARDWILARLAENIPAPAP"
     gene            complement(626..889)
                     /locus_tag="VN034_03610"
     CDS             complement(626..889)
                     /locus_tag="VN034_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HWV59716.1"
                     /translation="MHDGDAALALQALGWILSDEPRAERLLGLTGLAPDELRASLGEQ
                     ATLAAILSFLTGHENDLVACADALQVPPASIAAAAQRLEGTTA"
     gene            967..1329
                     /locus_tag="VN034_03615"
     CDS             967..1329
                     /locus_tag="VN034_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV59717.1"
                     /translation="MGKTILVVEDNELNLRLFCDLLNAHGYSAHPVRDGRDALAKARE
                     VSPDLIIMDIQLPHVSGLDLIGQMKADLTLRAVPIMAVTAYAGKGDEEQIKAAGAEAY
                     VSKPISVIKFIESVGAFA"
     gene            1492..>1733
                     /locus_tag="VN034_03620"
     CDS             1492..>1733
                     /locus_tag="VN034_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59718.1"
                     /translation="MKRLALTALIGATTLAAMPTEAEARSRHHRHGHYDRDHDRWQDR
                     REYRRHREGRYYRNCRTSGTTGLIVGGAAGALLGRE"
BASE COUNT          303 a          551 c          585 g          294 t
ORIGIN      
        1 ggggccgggg cggggatgtt ttcggcgagg cgcgccagga tccagtcgcg cgccgccgtc
       61 cagtcgtcga tgcgcgcatg cgcgtgcttc gcgggcgcga tcttgcccgc gatcgccggc
      121 tcgccgacga gatggagccg ccacacgtcg ggcgcctcct gcgcgaccga catatgatgg
      181 cccgcaaggt catcgatgaa gacagcgacc gaaggctgat attgctcgat caggcggcgc
      241 accggttcgc ccttgccccc gcggctgccg ataaccggcg cgtggaaatc gtggagcgcg
      301 agctgttcga tgcgcgcctg ctgatgttcg gggccgacgt tggtgagcac aacgatatcg
      361 gcctcggcgc cgatcgccgc catcgccgcg agcgcaccgg cgatcggata ttgccgcgtc
      421 atttcccggc ggaagaaacc atcgagcaac ggccacactt cggcggcctc gagcggtgtg
      481 ccgcattcct tgcgtttcag cgcgttcgcg aagctcgcgt cttcgatgcg gaagatcacg
      541 ccatgttcgg cgtcgaccca ttcggcgaag gggacgacca tgtgcatcag cacctcgtcg
      601 caatcggtga tgacgagcgg gcggttcatg cggtggttcc ttcaagtctc tgggcggcgg
      661 cagcgatgct ggcaggcggc acctgcaacg catcggcgca ggcgacaagg tcgttttcgt
      721 ggcccgtgag gaacgacagg atcgccgcga gcgtcgcctg ctcaccgagc gaggcgcgca
      781 gttcgtcggg ggcgagcccg gtcaggccaa gcagccgttc ggcgcgcggt tcgtcgctca
      841 aaatccagcc gagcgcctga agcgccagcg cggcatcgcc atcgtgcaag tcgtgtgtcc
      901 ctaattgtgt catgccgccg attcgcctaa acgggccgcg tacgcaaggt ggggaacata
      961 agtaacatgg gcaagaccat cctggtcgtc gaagataatg agctcaacct tcgcctcttc
     1021 tgcgacctgc tcaacgccca tggctatagc gcgcatccgg tgcgcgacgg acgcgacgcg
     1081 ctggccaagg cgcgcgaagt gtcgcccgac ctgatcatca tggacatcca gttgccgcat
     1141 gtcagcgggc tcgacctgat cggccagatg aaggccgacc tgaccttgcg cgcggtgccg
     1201 atcatggcgg tcaccgccta tgcggggaag ggcgacgagg agcagatcaa ggcggcgggc
     1261 gccgaagcct atgtgtccaa accgatttcg gtgatcaagt tcatcgaaag cgtcggagcc
     1321 ttcgcctagc cttttttcga tcgcccgacg tcgattgcga cgggccgccc aaaatcatcg
     1381 gcgagtcgct ggcgcgggcg gtgagcgcac ggccggttca gccaccggcc agctttcttt
     1441 tcctaatcct gatcgcgggg gttcaggatg gaaggacata agatgttttt gatgaagagg
     1501 ctggccttga cggcgttgat cggcgcgacg acgctggccg ccatgccgac cgaagccgag
     1561 gcgcgcagcc gccaccatcg gcacgggcat tacgaccgcg accatgaccg ctggcaggat
     1621 cgccgcgaat atcggcgtca ccgcgaaggc cgatattatc ggaactgccg taccagcggc
     1681 acgacggggc tgatcgtcgg cggcgccgcc ggcgccctgc tgggccgcga aat
//
LOCUS       DATMSU010000136        10122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_97508, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000136 DATMSU010000000
VERSION     DATMSU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97508"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VN034_03625"
     CDS             complement(<1..291)
                     /locus_tag="VN034_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase"
                     /protein_id="HWV59719.1"
                     /translation="MEGRPLTTAPADPPLESGADAAPLDDTIEPGKRLIRLPADKGLS
                     LGDRVANAITRLTWRTPLHAFRLKGRFPLKLLGVPADPVPGDTRAGTAIRAGH"
     gene            complement(295..966)
                     /gene="rpe"
                     /locus_tag="VN034_03630"
     CDS             complement(295..966)
                     /gene="rpe"
                     /locus_tag="VN034_03630"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWV59720.1"
                     /translation="MTGSTTPVRIAPSILSADFAKLGEEVRAIEAAGADWVHIDVMDG
                     HYVPNLTIGPMVVKALRPHSTLPFDVHLMISPVDHFLDAFAAAGADIITVHPEAGPHI
                     HRTVQHIKSLGKQAGVSLNPATPAKMLDYLIDDIDLVLIMSVNPGFGGQSFISSQLRK
                     IEAVKKMIEKSGRDIRLEVDGGIDAGTAPLAIDAGADVLVAGTATFKGGPDAYADNIR
                     RLRGA"
     gene            complement(1053..1295)
                     /locus_tag="VN034_03635"
     CDS             complement(1053..1295)
                     /locus_tag="VN034_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59721.1"
                     /translation="MLHLLLDKRPWFRAKTHGYGTGLPIAWQGWLFLALHIALIAGLA
                     VLLRERPVLMTIAIVLAALAPMPIYRARTEGGWRWR"
     gene            complement(1297..1500)
                     /locus_tag="VN034_03640"
     CDS             complement(1297..1500)
                     /locus_tag="VN034_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HWV59722.1"
                     /translation="MPASARKAFALRLDPALYAAVERLAAAELRSANAEIEILLREAL
                     ARRGVSVKQSQPPKRGRPPKEGT"
     gene            complement(1511..2413)
                     /locus_tag="VN034_03645"
     CDS             complement(1511..2413)
                     /locus_tag="VN034_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWV59723.1"
                     /translation="MVETGTPALNRSREARANTQSGYLMLFIWLALLGLAAWAGISNA
                     NAEVMVAWKWALVVAAGIAGTLILCGFYLINPNEAAAIQLFGAYKGTDRQEGLRWVLP
                     WLTRKKIAVRANNVISDKIKVNDLRGNPIEMAAQVVWRVTDTAQALFDVDDYKEFVMA
                     QIEAAVRAIGSRYPYDDIEHQEITLRGNHDEVGVELRKALIERLTVAGITVDECGLTH
                     LAYAQEIAGAMLRRQQAEAVIAARKKLVEGAVTMVEMALTHLSEKNVVELDDERKAAM
                     VSNLMVVLCGERDTQPVVNTGSLY"
     gene            complement(2581..3756)
                     /locus_tag="VN034_03650"
     CDS             complement(2581..3756)
                     /locus_tag="VN034_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59724.1"
                     /translation="MRNCLAICMALGMAAPASAQQALGANFNEHYEDIDYRDLDKADA
                     RWVRLFLTMPQLESTAAADHGAVKTILETGRRGYKTILSLKFPRNRRDFPAPGSAEMA
                     REMAWLDAVLPQVMGKIDILVIGNEPYIESRRVDRDLDLNAYYEAMAKRAIAFRKAKC
                     GANCQTALYMGALNRLDLAENRTPSAERWMAFVRSTPEIAGVDIHAHLPEMEGTRKFV
                     DYIIPRMRPDQKFLALEFSIVRWWEQHMKAPVPASYAAAYGLPADTRNWQVIRTALES
                     PFAKAQWDAFLASSPWFESRKHYLRHQMKIFRDTGRLAVATYGFKQGSSMGRNFGPKT
                     TPWLLNSVVAARTIRPNPDGSAAFNYAWIDDFRTLQKTEPVAPPSWRGKASPQRADP"
     gene            4002..6668
                     /locus_tag="VN034_03655"
     CDS             4002..6668
                     /locus_tag="VN034_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59725.1"
                     /translation="MASGNINLLIGASAFALMAVPGVAGAQEAQGSEDAAEAIVVTGF
                     RQSLESAQAVKQESDQIVDSVLAEDIGKLPDQTVAETAARITGVSVERSNGEASAVRV
                     RGLPDLTTTYNGREVFTAQGRYVQLQDFPSGTIGRIDVYKTSAANLPEAGIAGEIDLK
                     AQKPFNFKGMRIAGGVAGVHWRQSQQLGFDANLLLSNRWETGIGEIGLLVGGSYAQNK
                     AGDSLRQTSLAIQERGATAAFARARYPADINFDTVASDRVRSSVSTVLQWRPNPDLEF
                     YGDFLYQGFHSEGGARAVRVQAGSGATISDVTYCPGSTLLICQMTATGGNPVTSYASA
                     QGSDTDTYHGGGGFIWKTGGARITGDVGYTDSTFTTANFQLNSRLNAVPTRTFNFAEP
                     GGGGSAQIIGVNLADPANWRILSYNEGGSQAAGRGWQGRLDADIPVAVSIFDRLQVGV
                     RYTDRDARAESYNVPQSRGTFPTADGGLADLQFTALPIDFYNVSPAFKGDPTDHPRTW
                     IAPERDSIYDYRDFFRTLAGEPAGTPERVPQYWANERTYAAYAQTHYEFEIGTVRIDG
                     QIGVRAVRTENDIRTAQNSTTGKSSYEDYLPNISTRIRLTPNLQARFAFTKTSSRPGF
                     SQFNPVVTVAGGCAAAPTCASSGNPDLQPIKSTNYDASLEYYFSRSGSATVQIFRRDV
                     KGFINNTTTIITDPDIAPGEIALTRPENGQKGYIQGVEVGFRSFLKIASLPEWMQNFG
                     VLANYTYLDHKTELAQSSAATLPGLQPIANTSSHLANAQLFYETKKLSLRASYNYRSK
                     FYEYGITNDPEVARLGGPQLTLPTTEKGRGILDLSASINPTDFMSINFSASNVLGTPV
                     RTSRVFNARGESYDFQVGYRETVYRLGVRFRI"
     gene            complement(6743..7930)
                     /locus_tag="VN034_03660"
     CDS             complement(6743..7930)
                     /locus_tag="VN034_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWV59726.1"
                     /translation="MRKFWKTLAAPTMALLLAGAAAPDGTWTPAWTASMWQSGNPRQE
                     VAVDNATISFAVRVGADGKALRIRLSNEFGAAFRIGAASIRLRDGQTVPVTFGGKAAT
                     EVSANTSIVSDPVRLTVKSFDVVDVSLYLPGSVRFNTVHAAGGAKTQISAPGDHSRAP
                     FVAARRTDNRPLLASVDVLGDATRPVVVAFGDSITDNPGCAIDATPICRWSDVLGRRL
                     AKAGKPHVVVTQAISGNRVISRGTGPSAVERFDRDVLALPGVTHVVLLEGINDIGGSG
                     RARPDGTSTPTITDAELIAGYRDLVSRAHQRGIKVIAMTILPFEGAGYHSATGEAMRT
                     RINAWIRSSGTFDAVVDMEKVVADPANPRRLAPALHRGDFLHPNGEGEFAMGEAIPLD
                     LFD"
     gene            complement(8064..8852)
                     /locus_tag="VN034_03665"
     CDS             complement(8064..8852)
                     /locus_tag="VN034_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWV59727.1"
                     /translation="MRLELPLLHPPLQVRSEGSRLAERSTATAQERPELGRNLTYGLL
                     EFLGRGIVTGRFDDAPFPIEAELVRRYGVSLTVTREAVKMLCAKGLLTSRPRQGTTIL
                     PTSSWNLFDPDVLRWLVERKLEIGLLRQFNQLRAAIEPEGAALAARFASADHLAAIAQ
                     ALARIEAAKAGWDDPVAAGIAFHISILYATQNPFYAQFRDVVGSALQTSIRFGASFHD
                     SATNVADYHAVYEAIRAADADRARGAMRLLIDRVLGCIERADRR"
     gene            complement(8849..9835)
                     /locus_tag="VN034_03670"
     CDS             complement(8849..9835)
                     /locus_tag="VN034_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HWV59728.1"
                     /translation="MPPEVPPGAPERPYVDPAKAYLFAHMTKEHYGVLYYSVSLDGLN
                     WRRLNGGGPVSQEYHGHASITRMPDGRYILVGNKSDSDPFVRFWVSSDLIHWRPHGTY
                     KPDLSQVRGLSNPLQRLGAPKLFYDKASAKYLLTWHTPNVPHTPADPERYWASQRTLY
                     VLSSDLKTFEGPPKLLFDWDIATIDTVIVPGDDGQGYCALVKDERYPSYNWPTGKTVR
                     ISCAATLLGPYPMPGAPVSPNFREAPTIVRAPNNRDWLLYYEQYAGVSYGLSKAPRLE
                     GPWVQVQGNSGVPRWNRFEMPVGTRHGSMIEIDRAQYDAIVAAFPGKAAVAP"
BASE COUNT         1833 a         3212 c         3245 g         1832 t
ORIGIN      
        1 atgcccggcg cggatcgcgg tgcccgcgcg cgtgtcgccc ggcaccggat cggccggcac
       61 gccgagcagc ttaagcggaa aacgcccctt cagccggaac gcatggagcg gggtgcgcca
      121 ggtcagccgc gtgatcgcat tggcgacgcg gtcgccgagc gacagtccct tgtcggcggg
      181 caggcggatc agccgcttgc cgggctcgat cgtgtcgtcg agcggcgcgg cgtcggcacc
      241 ggattcgagc ggtgggtcgg cgggggcggt ggtcagcggt ctcccctcca tcgatcaggc
      301 tccgcgcagg cgccggatat tgtcggcgta agcgtcgggc ccgcccttga aggtcgcggt
      361 gcccgcgacg agcacatcgg cgcccgcgtc gatcgcgagc ggcgcggtgc ccgcgtcgat
      421 gccgccatcg acttccagcc ggatatcgcg acccgatttc tcgatcatct ttttgaccgc
      481 ctcgatcttg cgaagctggc tcgaaatgaa gctctggccg ccgaagcccg gattgacgct
      541 catgatcagg acgaggtcga tgtcgtcgat cagatagtcg agcatcttcg cgggcgtcgc
      601 gggattaagc gacacgcctg cctgcttgcc gagcgatttg atgtgctgga cggtacggtg
      661 gatatgcggc cccgcctcgg gatgcacggt gatgatgtcg gcgccggcgg cggcaaaggc
      721 gtcgaggaaa tgatcgacgg gcgaaatcat cagatggacg tcgaagggca gggtcgaatg
      781 cgggcgcagc gccttcacga ccatcgggcc gatcgtcaga ttgggcacat aatggccgtc
      841 catcacgtcg atatggaccc aatcggcccc cgccgcctcg atcgcgcgca cttcctcgcc
      901 gagcttcgcg aaatcggcgc tcaggatcga cggggcgatg cggacggggg tggtggagcc
      961 ggtcatggac cggccttaac catctagcga gtcgggagca agcgggcccg aggcgctatc
     1021 gacagctatc cccaagaaaa gtggcgtcgc gcctatcgcc agcgccagcc gccttcggtg
     1081 cgggcgcggt agatcggcat cggcgcgagc gccgcgagca cgatcgcgat cgtcatcagc
     1141 accggccgct cgcgtagcag cacggcaagc cctgcgatca gcgcgatatg gagcgcgagg
     1201 aagagccagc cctgccaggc gatcggcagc cctgtgccat agccatgagt tttggcgcgg
     1261 aaccaggggc gcttgtcgag caacagatgc agcatgtcag gttccttcct tcggcggccg
     1321 gccccgtttc gggggttgcg attgcttgac gctcacaccg cgccgcgcca gcgcctcgcg
     1381 cagcaggatt tcgatctcgg cattggcgct gcgcaattcg gcggcggcca gccgctcgac
     1441 cgcggcgtag agcgccgggt cgaggcggag ggcgaaggct ttcctggcgg acgcgggcat
     1501 gcggacaaac tcagtagagc gaaccggtgt tgacgacggg ctgggtgtcg cgttcgccgc
     1561 acagcacgac catcaggttc gacaccatcg ccgccttgcg ctcgtcgtcg agctcgacga
     1621 cattcttttc cgagaggtgg gtgagcgcca tttcgaccat cgtcaccgcg ccctcgacca
     1681 gcttcttgcg cgccgcgatc accgcttcgg cctgctggcg gcggagcatc gcgccggcga
     1741 tttcctgcgc ataggcgagg tgggtcagcc cgcattcgtc gacggtgatg ccggcgacgg
     1801 tcagccgttc gatcagcgcc ttgcgcagct cgaccccgac ctcgtcatgg ttgccgcgca
     1861 gcgtgatttc ctgatgctcg atgtcgtcat agggatagcg cgagccgatc gcgcgcaccg
     1921 ccgcctcgat ctgcgccatc acgaattcct tgtaatcgtc gacgtcgaac agcgcctgcg
     1981 cggtatcggt gacgcgccag acgacctgcg ccgccatctc gatcgggttg ccgcgcaggt
     2041 cgttgacctt gatcttgtcc gaaatgacgt tgttcgcgcg caccgcgatc ttcttgcgcg
     2101 tcagccacgg cagcacccag cgcagcccct cctggcggtc ggtgcccttg taagcgccaa
     2161 acagctggat cgcggcggct tcgttgggat tgatcaggta aaaaccacag aggatcagcg
     2221 tgccggcgat gcccgcggcg acgaccagcg cccatttcca cgcgaccatc acctcggcat
     2281 tggcgttgct gatccccgcc caggcggcca gtccgagcag cgccaaccag atgaacagca
     2341 tcagatagcc gctctgcgta ttcgcacgtg cctcgcggct gcggttcagc gcgggcgtcc
     2401 ctgtttcgac catgtttccc tccgaatcac ccgatataaa gatgatatca tatttatatc
     2461 gaatcggacg aaggtcaatg gatttcggag gcggcgtggg ggcttggaac ggccccgccg
     2521 ggacaagctg accgtgacta tgagcaaagc ctgtttttcg accctgatcc ggacgcgaga
     2581 tcaaggatcg gcgcgctggg gcgacgcttt cccccgccat gaaggcggcg ccaccggctc
     2641 cgttttctga agagtgcgga aatcgtcgat ccaggcatag ttgaacgcgg cgctcccgtc
     2701 gggattggga cgtatcgtgc gcgcggcgac gacgctgttg agcagccacg gcgtggtctt
     2761 cggtccgaaa ttgcgtccca tcgaactgcc ttgcttgaaa ccataggtcg ccacggccag
     2821 gcggcccgta tcgcggaaga ttttcatctg gtggcgtaga taatgcttgc ggctttcgaa
     2881 ccatgggctg cttgcgagaa acgcatccca ctgcgccttg gcgaagggcg attcgagagc
     2941 ggtgcgaatg acctgccaat ttcgtgtgtc ggcgggaaga ccgtaggcgg cggcgtagga
     3001 agccggaacg ggcgccttca tatgctgctc ccaccaacgc acgatcgaga actcgagcgc
     3061 cagaaacttc tggtcgggcc gcattcgcgg gatgatgtaa tcgacgaatt ttcgcgtccc
     3121 ttccatctcg ggcaggtgcg cgtggatatc gaccccggca atttcgggag tgcttcgtac
     3181 gaacgccatc caccgctctg ccgacggggt gcggttctcg gcgaggtcga ggcgattgag
     3241 cgcgcccatg taaagcgcgg tctggcagtt cgcgccgcac ttggctttgc gaaacgcgat
     3301 cgcgcgcttc gccatcgcct cataataggc attgaggtcg agatcgcgat cgacgcgtcg
     3361 gctctcgata taaggctcgt tcccgatcac gaggatatcg atcttgccca tcacttgcgg
     3421 caggacggcg tcgagccaag ccatttcgcg cgccatttcc gcgcttccgg gggcgggaaa
     3481 gtcgcgccgg ttgcgcggaa acttcagcga caggatcgtc ttgtagccgc gccggcccgt
     3541 ctcgaggatc gtcttgaccg cgccatggtc cgccgcagcg gtcgattcga gttgaggcat
     3601 ggtcaggaac agccgaaccc agcgggcgtc cgccttgtcc agatcgcgat agtcgatatc
     3661 ctcataatgc tcgttgaaat tcgcgccgag cgcctgctga gccgatgccg gcgcagccat
     3721 gccgagcgcc atgcaaatgg ccagacagtt ccgcatcgct ctctccctca ttgtcgctat
     3781 ccccctaaaa gtggatcgtt aatccgcgtt tgccagctaa ttggcctgac aaattcaatc
     3841 ccgcagttgg cgaattttgg tgctgccggc tcgcccggcc ctcttggaag gcaacaggcg
     3901 gcccatcaaa attcggtcgg gccagtttgt atgtattgtc ataccaatca ttgtctgata
     3961 attcagatat gtcatctaga aaatgtgttt gggagggaaa aatggcttcg ggcaatatca
     4021 atctcttgat cggggcctcg gcgttcgcgc tcatggccgt gccgggagtg gcgggtgcgc
     4081 aagaggcgca ggggtctgag gacgcggcgg aagcgatcgt cgtcacgggt tttcgtcaga
     4141 gtctcgagag cgcccaggcg gtcaagcagg aatcggatca gatcgtcgac tcggtcttgg
     4201 ccgaggatat cggcaagctt cccgatcaga ccgttgcaga gaccgcggcg cggatcacgg
     4261 gtgtatcggt cgaacgctcc aacggcgagg catcggcggt gcgcgtgcgc gggcttcccg
     4321 atctcacgac cacttataat ggccgggagg tgttcaccgc gcagggccgc tacgtccagt
     4381 tgcaggattt cccctcgggc acgatcggcc gaatcgacgt ctacaagacg agcgcggcca
     4441 atcttcccga agccgggatc gcgggcgaga tcgacctcaa ggcgcaaaag cctttcaatt
     4501 tcaaaggtat gcgcatcgcc ggtggtgtcg ccggggttca ctggcgacag tcgcaacaac
     4561 tggggttcga cgccaatctg ctgctgagca atcgctggga aaccggcatc ggcgaaatcg
     4621 gcctcctcgt gggcggttcc tatgcccaga acaaggccgg ggattcgctg cgccagacat
     4681 cgctcgcgat tcaggagcgc ggcgcgacgg ccgctttcgc gcgcgcgcgc tacccggccg
     4741 atatcaattt cgacacggtg gcttccgatc gtgtccgttc gtcggtcagc accgtcctgc
     4801 aatggcggcc gaatcccgat ctcgaattct acggcgactt cctctatcag ggtttccaca
     4861 gcgaaggcgg cgcgcgcgcg gtgcgcgttc aggcggggtc gggcgcgacg atctccgacg
     4921 tgacctattg ccccggcagc acgttgctga tctgtcagat gacggcaacg ggcggcaatc
     4981 cggtcaccag ctatgcgtcg gcgcagggat cggacaccga tacctatcac ggcggcggcg
     5041 gcttcatctg gaagaccggc ggcgcgcgga ttaccggcga cgtcggctat accgattcga
     5101 ccttcaccac cgcgaacttc cagctcaatt cgcggctgaa cgcggttccg acccgcacgt
     5161 tcaactttgc ggagcccggc ggcggtgggt cggcgcaaat catcggcgtc aatctggccg
     5221 acccggcgaa ctggcgcatt ctgagctata atgaaggcgg gtcgcaggcc gccggccggg
     5281 gctggcaggg ccggctcgat gccgatatcc cggttgcggt gtcgatcttc gacaggcttc
     5341 aagtgggcgt gcgctatacc gaccgcgatg cccgcgccga atcctataat gtgccgcaaa
     5401 gcagggggac gtttcccacc gccgacgggg gactcgccga tttgcagttc acggcgctgc
     5461 cgatcgactt ctacaatgtg tcacccgcgt tcaaaggcga ccccaccgat cacccgcgca
     5521 cctggatcgc gccggaacgg gacagcatct acgattatcg cgatttcttt cgcacgctgg
     5581 caggcgagcc ggccggcacg ccggagcggg tgccgcaata ttgggcgaac gagcggacct
     5641 atgccgccta tgcgcagacg cattatgaat tcgaaatcgg cacggtgcga attgacggcc
     5701 agatcggcgt tcgtgcggtc cgcaccgaaa acgacatcag gaccgcccaa aactcgacca
     5761 caggcaagag cagctatgaa gattatctgc ccaacatcag cacgcgcatc cgcctgacgc
     5821 ccaatctgca ggcgcgcttc gccttcacga agacgtcgtc gcgccccggc ttcagtcagt
     5881 tcaatcccgt cgtcacggtt gcgggcggct gcgccgcggc ccccacctgc gccagcagcg
     5941 ggaatcccga tctgcagccg atcaaatcga ccaactatga tgcatcgctc gaatattatt
     6001 tctcgcggtc gggatcggcg acggtgcaaa tcttccgccg cgatgtgaag ggcttcatca
     6061 acaacacgac gacgatcatc accgatcccg acatcgcgcc cggcgaaatc gcgctcacgc
     6121 ggcccgagaa cgggcagaag ggttatatac agggcgtcga ggtcggtttc cggagcttcc
     6181 tgaagatcgc ttcgctgccg gaatggatgc agaatttcgg cgttctggcg aactacacct
     6241 atctcgatca caagaccgag ctggcgcaaa gctcggcggc gaccctgccg ggtcttcagc
     6301 cgatcgccaa cacatcgtcg catctggcca atgcccagct attctacgaa acgaagaagc
     6361 tctcgctccg ggcgtcgtac aactaccgct cgaaattcta cgaatatggc atcaccaacg
     6421 accccgaagt ggcgcggctc ggcggtcccc agctgacctt gccgaccacc gaaaaagggc
     6481 gcgggatcct cgatctttcg gcctcgatca acccgaccga cttcatgagc atcaatttca
     6541 gcgcgtcgaa tgtgctgggc acgccggtac ggacatcgcg cgtgttcaac gcccgcggcg
     6601 agagttacga tttccaggtc ggctatcgcg agacggtcta tcgtctgggc gtgcgcttcc
     6661 gcatctgatc cggccgacgc caaccgaaac catgactggc gccgcacgaa acctcgtgcg
     6721 gcgccattat tttcgatcgg ccctagtcga aaaggtccag cgggatggct tcgcccatcg
     6781 cgaattcgcc ctcaccattg gggtggagaa aatcgccgcg atggagcgcc ggggccagcc
     6841 gccgcggatt ggcggggtcg gcgaccacct tctccatgtc gaccaccgcg tcgaacgtcc
     6901 cggaagaccg gatccacgcg ttgatccgcg tccgcatcgc ctcgcccgtc gcgctatgat
     6961 agcccgcacc ctcaaagggc aggatcgtca tggcgatgac cttgatcccg cgctgatgcg
     7021 cgcgcgacac gagatcgcga tatcccgcta tcagctccgc gtcggtgatg gtcggtgtac
     7081 tcgtcccgtc gggccgcgcc cgccccgatc cgccgatgtc gttgatcccc tcgagcagca
     7141 cgacatgggt gacgcccggc agggccaaga cgtcgcggtc gaaccgctcg accgcgctcg
     7201 ggccggttcc ccggctgatc acccggttgc ccgagatcgc ctgggtcacc acgacatgcg
     7261 gctttccggc cttggccagc cggcggccga ggacgtcgct ccagcggcag atcggcgtcg
     7321 cgtcgatcgc gcagcccgga ttatcggtga tcgaatcgcc aaaggcgacg accaccggac
     7381 gcgtcgcatc gcccagcacg tcgacgctgg cgagcagcgg ccggttgtcc gtccggcgcg
     7441 ccgcgacgaa gggcgcgcgg ctatggtcgc ccggcgccga gatctgcgtc ttcgcaccgc
     7501 ccgcggcatg gaccgtgttg aaccgcacgg atcccggcag atagagcgac acgtcgacga
     7561 cgtcgaaaga cttgacggtc agccgaacgg ggtcgctgac gatgctcgta ttcgccgaga
     7621 cctcggtcgc ggctttgcct ccgaaggtga ccggaacggt ctggccgtcg cgcagccgga
     7681 tactcgccgc tccgatccga aaggcggcgc caaattcgtt cgaaaggcgg atccgaaggg
     7741 ccttgccgtc tgccccgacg cgcaccgcga agctgatcgt cgcattgtcg actgcgacct
     7801 cctgccgcgg attgcccgat tgccacatgc tcgcggtcca ggccggagtc catgtcccgt
     7861 ccggcgcggc cgccccggcg aggagcagcg ccatcgtcgg cgcggccaga gtcttccaaa
     7921 actttctcat tcaatctccc cttcgctaga ctggccacgg tcgcttcagc ttgcgacacc
     7981 tagataccgg atctggaact cgtcctcgcc ccatgcgcaa gaggcattat cagattttgg
     8041 cttttccttc ggcgtcggca gcatcagcgg cgatcggcgc gctcgataca gccgagcacg
     8101 cgatcgatca gcaatctcat cgccccgcgc gcgcggtcgg catcggctgc ccggatcgcc
     8161 tcgtacaccg cgtgatagtc agcgacgttc gtcgcgctgt catggaagct cgcgccgaac
     8221 cggatggagg tttgcagcgc ggagccgacg acgtcgcgaa actgggcata gaagggattc
     8281 tgcgtcgcgt agagaatgga aatatggaaa gcgatgcccg ccgcgacggg atcgtcccat
     8341 cccgccttcg cggcctcgat ccttgccagc gcctgtgcga tcgccgccag atggtcggcg
     8401 gacgcgaagc gcgccgccag cgcggcaccc tcgggctcga tcgcggcgcg caactggttg
     8461 aactggcgca gaaggccgat ctcaagcttg cgttcgacca gccagcgcaa cacgtcggga
     8521 tcgaagagat tccaggacga agtgggaaga atcgtcgttc cctggcgcgg ccgcgacgtc
     8581 aacaggccct tggcgcacag catcttcacg gcttcgcggg tcaccgtcag gctgactccg
     8641 tatcgccgga cgagttccgc ctcgatcggg aagggcgcat cgtcgaaccg gccggtgacg
     8701 atgcccctgc cgagaaattc gagaagccca tatgtcaggt tgcgccccag ctccggccgt
     8761 tcctgcgcgg tcgccgtcga tcgttccgca aggcggcttc cttcggatcg aacctgcagg
     8821 ggcgggtgca gcaggggcag ctccaacctc atggtgcgac ggccgccttg ccgggaaagg
     8881 ccgcgacgat cgcgtcatat tgcgcgcggt cgatctcgat catcgagccg tgccgtgtac
     8941 caacgggcat ctcgaagcgg ttccagcgcg gcactccgct attgccctgc acctgcaccc
     9001 atgggccttc cagccggggc gctttggaca ggccgtaact taccccggca tattgctcgt
     9061 aatagagcag ccaatcgcga ttgttcgggg cgcggacgat cgtcggtgcc tcgcggaaat
     9121 tcgggctgac cggcgcgccc ggcatcggat aagggccgag aagcgtggcg gcgcagctga
     9181 tccgcacggt ctttcccgtt ggccaattat atgacggata acgctcgtcc ttgaccagcg
     9241 cgcaatagcc ctgtccgtcg tcgcccggaa cgatgaccgt gtcgatcgtc gcgatatccc
     9301 aatcgaacag cagcttgggc ggaccttcga aggtcttgag atcgctcgac aggacataga
     9361 gggtgcgctg gctggcccaa tagcgttccg gatcggcggg cgtgtggggg acattgggcg
     9421 tatgccaggt cagcagatat ttcgccgatg ccttgtcata gaatagtttg ggcgcgccga
     9481 gccgctgcag cggattggag aggccccgca cctgcgaaag gtcgggctta tatgtgccgt
     9541 gcgggcgcca gtggatcagg tcggacgaaa cccagaagcg gacgaacgga tcgctgtcgc
     9601 tcttgttgcc gaccaggatg tagcgcccgt cgggcatacg ggtgatcgag gcatggccgt
     9661 gatattcctg cgagaccggc ccgccgccgt tgaggcggcg ccagttcagc ccgtcgaggc
     9721 tgacggaata atagaggacg ccataatgtt ccttggtcat gtgcgcgaag agataagcct
     9781 tggccggatc gacataaggc ctttcgggcg cgccgggcgg cacttcggga ggcaaggccc
     9841 ccgtcgcttg cgccagagcg ggcaaagggg cggttgaaag cgcaagccag atcaagagtg
     9901 cgcgcaggcg gagagagcct cgcaagatcg gtactggcgt gtggaaattc atgtgtgtct
     9961 ccccggtttc gatgcgcagc gaaaagggta ttgcatcggc tcacttaaga ttggtaagat
    10021 aaattctatt atagatacct gacagaaaca aggaggattg catggccact cgacggacca
    10081 aggggctagc gatgggttcg gtactggcca tggcgatcgc gg
//
LOCUS       DATMSU010000137         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_49839, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000137 DATMSU010000000
VERSION     DATMSU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49839"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VN034_03675"
     CDS             complement(<1..595)
                     /locus_tag="VN034_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWV59729.1"
                     /translation="MSIGALHVARTGLDAQGFRMQVIANNLANVNTTGFKRDRASFET
                     LSYQLMTQAGAPSTAENRYATGLNLGTGVALNGTARIDTQGAFQTTGNGLDVAIDGAG
                     YFQVEMPDGRIGYTRAGNFGRAPDGTIVTSDGKPLTPAIQLPEDASNVTIGLDGTVSA
                     TAADGTALELGRIEIARFANPAGLQAIGGNMLVETQAS"
     gene            complement(608..1351)
                     /gene="flgF"
                     /locus_tag="VN034_03680"
     CDS             complement(608..1351)
                     /gene="flgF"
                     /locus_tag="VN034_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HWV59730.1"
                     /translation="MDRLIYTSLTAMRGSMSRQTAIANNLANAQTPGFRADIANAQSL
                     WLDGGGLDARAMASEEVLGADMRAGTVTQTGRDLDIAMQGDALLVVQAENGEEAYTRR
                     GDLQISPSGLLTTGDGHPVQGTQGPVTIPPADAISIDQEGRVWVVPQGGDPENPQEVD
                     RLRLAGPAGSEIVKGLDGLFRVKGGGILPDDPEARLLTRSIEGSNVTATTALVEMIEA
                     SRSWDTQLKMISDVRDMDSATANLMQLPR"
     gene            complement(1384..2211)
                     /locus_tag="VN034_03685"
     CDS             complement(1384..2211)
                     /locus_tag="VN034_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook basal-body protein"
                     /protein_id="HWV59731.1"
                     /translation="MSFFTSLSGLKASQTDMSVISNNIANAGSVGFKRSKAQFGDIFA
                     SSPTQSTKTIAGQGTRLNGITQQFTQGSYEASEKTLDMAIVGEGLFIVKGNPPTEAIT
                     YTRNGSFEPTPDGYVIDTTGAKLQLLPVDADGNVTNNTLAGAFDMQLPTGAPSDPTSS
                     LVNVSIGLDGLVTATFANGESEKLGKVAMATFPTMSGLRPTGDAHWQSTGESGPPTID
                     GPTSGAMGAVRSGALERSNVDITEELVMLMSAQRNFQANAKAIEGASQLTQTIIGMR"
     gene            complement(2216..2518)
                     /locus_tag="VN034_03690"
     CDS             complement(2216..2518)
                     /locus_tag="VN034_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HWV59732.1"
                     /translation="MGVNSITNNNPVIHPKGTSQQMDQGDFLRLMTAQLSQQDPFNPV
                     DNQQMVAQMAQFSSLAGISETNNVLAQISEQLTAQTQLLKDIKAATPAPAPTTQEG"
     gene            complement(2520..2930)
                     /gene="flgC"
                     /locus_tag="VN034_03695"
     CDS             complement(2520..2930)
                     /gene="flgC"
                     /locus_tag="VN034_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWV59733.1"
                     /translation="MTMNGNFSVFDISGRAMSAQLVRLNTTASNLANAGTVAGSEAEA
                     FRSLKPVFRTVMDDHGRATVQIDQVTTSKMAPSKRHDPSNPLADADGNVWEAAVDSAA
                     ELVEMVETARQYQNNVQVLETAKGLINETLRMGQ"
     gene            complement(2927..3268)
                     /locus_tag="VN034_03700"
     CDS             complement(2927..3268)
                     /locus_tag="VN034_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HWV59734.1"
                     /translation="MMASEKLFGLHATALQLRSQRMMMLASNIANAATPNYKARDIDF
                     AKALDLAQQGASTDGAISYRVPVQASLDGNTVEMATEQTAYAENALAYRSSLSFLSGR
                     INTLSRALKGE"
BASE COUNT          504 a         1068 c         1147 g          678 t
ORIGIN      
        1 ccgaggcctg cgtttcgacg agcatgttgc cgccgatcgc ctgcagcccc gccgggttgg
       61 cgaagcgcgc gatctcgatc cggccgagtt cgagcgcggt gccgtcggcg gcagtcgccg
      121 aaacggtgcc gtcgagaccg atcgtcacat tgctcgcatc ctcgggaagc tggatcgcgg
      181 gggtcagcgg cttgccgtcc gaggtcacga tcgtgccgtc gggggcgcgg ccgaaattgc
      241 ccgcacgggt atagccgatg cgcccgtcgg gcatttcgac ctggaaatag ccggcgccgt
      301 cgatcgcgac gtcgagcccg ttgcccgtcg tctggaacgc gccctgcgta tcgatgcgcg
      361 cggtgccgtt gagcgccacg ccggtgccga ggttgagccc tgtcgcatag cggttttccg
      421 ccgtcgaggg cgcacccgcc tgcgtcatca attgatagct cagcgtctcg aagctcgcgc
      481 ggtcgcgctt gaagcctgtc gtgttgacgt tggcgaggtt gttggcgatc acctgcatcc
      541 ggaagccctg ggcatccaga ccggtccgcg cgacgtgcaa ggcaccgata ctcatttctt
      601 attctcctta acgggggagc tgcatcagat tggcggtggc gctgtccatg tcgcgcacgt
      661 cgctgatcat cttcaattgc gtgtcccagc tacggctcgc ctcgatcatc tcgacgagcg
      721 cggtggtggc ggtgacgttc gatccctcga tcgagcgggt gagcagccgc gcttcgggat
      781 cgtcgggcag gatgccgccg cccttgacgc ggaacaggcc gtcgagcccc ttgacgattt
      841 ccgaccctgc gggacccgcg agccgaagcc ggtcgacttc ctgcggattt tcggggtcgc
      901 cgccctgcgg cacgacccac acgcggcctt cctggtcgat gctgatcgcg tcggcgggcg
      961 ggatcgtcac cgggccctgc gtcccctgca cgggatggcc gtcgccggtg gtcaggagcc
     1021 cgctcggcga gatttgcagg tcgccgcgcc gcgtataggc ctcttcgcca ttctcggcct
     1081 gcacgacgag caacgcgtcg ccctgcatcg caatgtcgag gtcgcggccg gtctgggtga
     1141 cggtgcccgc acgcatgtcg gcgccgagca cttcctcgga cgccatggcg cgcgcgtcga
     1201 gcccgccgcc gtcgagccac agcgactgcg cattggcgat gtcggcgcgg aagcccggcg
     1261 tctgggcgtt cgccagattg ttggcgatcg ccgtctgccg ggacatgctg ccccgcatcg
     1321 cggtgaggct ggtatagatg aggcggtcca tctggatact cccggcaggc ggcggtcagc
     1381 gcgtcagcgc atgccgatga tcgtctgggt cagctgcgac gcgccctcga tcgccttggc
     1441 attggcctgg aaattgcgct gcgccgacat cagcatcacc aattcctcgg tgatatcgac
     1501 gttcgagcgt tcgagcgcgc ccgaacggac cgcacccatc gcgccgctgg tcgggccgtc
     1561 gatcgtcggc ggaccgcttt cgcccgtcga ctgccagtgc gcgtcaccgg tcgggcgcag
     1621 gcccgacatc gtcgggaagg tcgccatcgc gaccttgccg agcttctcgc tctccccgtt
     1681 cgcgaaagtc gcggtgacga ggccgtcgag gccgatcgac acattgacca gcgacgaggt
     1741 cggatcggac ggcgcgcccg tggggagttg catgtcgaac gcgccggcga gcgtgttgtt
     1801 ggtgacattg ccgtcggcgt cgacaggcag cagttgcagc ttcgcgcccg tcgtgtcgat
     1861 gacatagccg tcgggggtcg gttcgaacga tccgttacgc gtataggtga tcgcctcggt
     1921 cggcggattg cccttcacga tgaacaggcc ctcaccgacg atcgccatgt cgagcgtctt
     1981 ttcgctcgct tcgtacgagc cctgcgtgaa ctgctgggtg atgccgttga ggcgcgtgcc
     2041 ctggcccgcg atcgtcttcg tcgactgcgt cggcgacgag gcgaagatgt cgccgaactg
     2101 cgccttgctg cgcttgaaac cgaccgagcc ggcgttggcg atattgttcg agatgaccga
     2161 catatcggtc tgcgaagcct tgaggcccga aagcgacgta aagaatgaca tggacttatc
     2221 cttcctgggt ggtgggggcg ggcgccgggg tggcggcctt gatgtctttg agcagctgcg
     2281 tctgcgcggt gagctgctcc gaaatctgtg cgagcacatt gttcgtttcg ctgatcccgg
     2341 cgaggctcga gaattgcgcc atctgggcga ccatctgctg gttatcgacc gggttgaacg
     2401 gatcctgctg cgacagctgc gcggtcatca ggcgcaggaa atcaccctga tccatctgct
     2461 ggctcgtgcc tttggggtgg atcacggggt tgttgttggt gatgctgttg acgcccatct
     2521 tactgtccca tcctgagagt ttcgttgatc aggcccttcg cggtttcgag gacctggaca
     2581 ttgttctgat actggcgcgc ggtctcgacc atctcgacca gctcggcggc gctatcgacc
     2641 gcggcttccc agacgttgcc gtcggcatcg gcgagcggat tcgaagggtc gtggcgtttc
     2701 gacggcgcca ttttggaggt cgtgacctgg tcgatctgga ccgtcgcgcg gccatgatcg
     2761 tccatcacgg tgcgaaagac gggcttcaaa gagcggaagg cttccgcctc gctgcccgcg
     2821 accgtccccg cgttggcgag gttcgaggcg gtggtgttga gccggacgag ctgcgccgac
     2881 atcgcgcggc cgctgatatc gaagacggag aaattgccgt tcatcgtcat tcgcccttca
     2941 gcgcgcgcga cagcgtgttg atacgcccgc tgaggaaaga aaggctcgac cggtaggcga
     3001 gcgcgttttc ggcataggcg gtctgctcgg tcgccatctc gacggtgttg ccgtcgagcg
     3061 acgcctggac gggaacgcga tatgaaatcg cgccgtcggt cgacgcgcct tgctgcgcga
     3121 gatcgagcgc cttggcgaag tcgatgtcgc gcgccttgta attcggcgtc gcggcattgg
     3181 cgatgttcga cgcgagcatc atcatgcgct ggctgcgcag ctgaagcgcc gtcgcatgca
     3241 agccaaagag tttctcggat gccatcatcc cgttccttca tttgccgcca tccggcggcg
     3301 gggtctccgc ggtccggccg gctggcacgg acgcgcggaa atacaccaag gcatgatgca
     3361 atggccgtgc caaaagtctt ttttctgtat aattcag
//
LOCUS       DATMSU010000138         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_78052, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000138 DATMSU010000000
VERSION     DATMSU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78052"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1834)
                     /locus_tag="VN034_03705"
     CDS             complement(<1..1834)
                     /locus_tag="VN034_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWV59735.1"
                     /translation="MKLDFIPLDKLSIDPANMRAKGRDPDVSDILPSVRKRGVLQSLL
                     VRPAPDDGHFLVSAGRRRLTAANVVAREGGPARPLPCGILEDGDDADAIEASLLENLA
                     RVAPDEVSQWEAFVKLVKAGRKAEEIAADFAFEVATVRRILALGNLVPRIRALYRSGE
                     IDPTTVRQLTLASRSQQTAWLALFDDKDAYCPTGFRLKDWLFGGDAIKAAHALFDVEA
                     AGLAVIADLFDNEKLVADTDAFWALQKAEVEARKAAYLADGWSDVVILERAPRFSRWQ
                     HVATGKRKGGRVYIELHSTGEVEFHEGYLTEKEAAARARAEAGAPPERAKRPEVTGAI
                     NSYIDLHRHAAVRAELARHGGVALRALAAHVIASADHFRVDVQSQVCAREEVQESVET
                     CASETAFDERRRAVLAVLGFGAEDATVTARYCGRRQFAPLFARLLDLPDPVVLEIVAI
                     VMGETLMAGSEAVEMIGLHLGVDMAAHWQPDAAFWNLVRDKDVLGRMVAEVAGEEVAA
                     ANGGEKAKALKAIIADHLEGANGRAKVAPWVPKWMGFPPASYTARGGVASVEAGARML
                     HLLAEAAEAETDADEGGLTECASETGSVDCEGGGEGDGAGSEAPG"
     gene            complement(1925..2344)
                     /locus_tag="VN034_03710"
     CDS             complement(1925..2344)
                     /locus_tag="VN034_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2958 domain-containing protein"
                     /protein_id="HWV59736.1"
                     /translation="MSLLPYSLAFALNANRVASRIHAARGEAFDPWPVVKLFNPVGAA
                     TWIATEIDEDGDTLFGLADLGFGCPELGCFSLGEIRAVRLPFGLLIERDRHFDAMAPL
                     SVWTDEARRHGSITRAEAALRRRARDRLLPDPKERGS"
     gene            complement(2341..>2806)
                     /locus_tag="VN034_03715"
     CDS             complement(2341..>2806)
                     /locus_tag="VN034_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HWV59737.1"
                     /translation="RATIPVAEALLAASGADIRIGGNEAYYSPSGDYVALPPQQAFFE
                     QIDYYRTALHELGHWTGHPTRLGRDQTGGFASAAYGREELCAELASAFLCAALGIRPT
                     VRHADYIGAWLAILRADTRAIFKAASLASKAADYLMAYAPAEARGATKGEGQ"
BASE COUNT          426 a          983 c          947 g          450 t
ORIGIN      
        1 cgccgggggc ttcgctcccg gcgccatccc cttcgccgcc gccttcgcaa tccacggagc
       61 cggtttcgct cgcgcactcg gtcagccctc cttcgtcggc atcggtttcg gcctccgctg
      121 cctcggccaa cagatggagc atccgcgccc cggcctcgac gctcgcgacg ccgccgcgcg
      181 cggtatagga tgcgggcgga aagcccatcc acttgggcac ccacggcgcc accttcgcac
      241 ggccgttcgc gccttcgaga tggtcggcga tgatcgcttt gagcgccttc gccttctccc
      301 cgccgttcgc tgcggcgacc tcttcgcccg cgacctcggc gaccatcctg cccagcacgt
      361 ccttgtcgcg gacgaggttc cagaaggccg cgtcgggctg ccagtgcgcc gccatgtcga
      421 cgccgagatg aagaccgatc atctcgaccg cctcgctccc cgccatcagc gtttcgccca
      481 tcacgatcgc gacgatttcg agcaccacgg ggtcgggcag atcgagcagc cgcgcgaaca
      541 gcggcgcgaa ctggcggcgc ccgcagtagc gcgcggtcac cgtcgcatcc tccgcgccga
      601 agccgagaac cgcgagcacc gcgcggcgcc gctcgtcgaa ggcggtctcg gacgcgcagg
      661 tctcgacgct ttcctgcacc tcttcccgag cgcagacctg cgactggaca tcgacgcgga
      721 aatggtcggc gctcgcgatg acatgcgccg cgagcgcgcg gagcgcgacg ccgccatgtc
      781 tcgcgagttc ggcgcgcacc gccgcatggc ggtggaggtc gatatagcta ttgatcgcgc
      841 ccgtcacctc gggccttttc gcgcgttcgg gcggcgcccc cgcctcggct ctcgcgcgcg
      901 cggcggcttc tttctcggtc agatagcctt cgtggaattc gacctcgccg gtcgaatgca
      961 gctcgatata gacgcgcccg cccttgcgct ttcccgtcgc aacatgctgc caccgcgaga
     1021 aacgcggcgc gcgttcgaga atgacgacat cgctccagcc gtcggcgaga taggccgcct
     1081 tgcgcgcctc gacctcggct ttctggagcg cccagaaggc atcggtgtcg gcgacgagct
     1141 tctcgttatc gaacaggtcg gcgatcacgg cgaggcccgc cgcctcgacg tcgaacagcg
     1201 catgcgccgc cttgatcgcg tcgccgccga acagccagtc cttgagccgg aaacccgtcg
     1261 ggcaataggc gtccttgtcg tcgaacagcg ccagccacgc ggtctgctgg ctcctgctcg
     1321 caagggtcag ctgcctgacc gtcgtcgggt cgatctcgcc gctgcggtag agcgcgcgga
     1381 tgcgcggcac gaggttgccg agcgcgagga tacgccgcac cgtcgccacc tcgaaggcga
     1441 agtcggcggc gatctcctcg gccttgcgtc cggccttgac gagcttgacg aaggcctccc
     1501 actggctgac ctcgtcgggg gcgacgcgcg cgagattttc gagcaacgac gcctcgatgg
     1561 cgtcggcgtc gtcgccgtct tccaagatgc cgcacggaag cgggcgcgcg ggtccgccct
     1621 cgcgcgcgac gacattggcg gcagtgaggc gccgccgacc tgccgaaaca aggaaatgcc
     1681 cgtcgtcggg ggccggacgg acgagtagcg attgcaacac gccgcgcttg cgcacgctcg
     1741 gcagaatatc gctcacgtcg gggtcgcgcc ccttcgcccg catattggcc gggtcgattg
     1801 acagcttgtc gagcggaata aaatcgagtt tcatggcact tgctcctcat gtgagcgccg
     1861 gttccgtttt gaggtggatg cgagacggga ccggcttcga tgacggcgag aacgcgccgc
     1921 cgcgtcagct gcctctctcc ttcggatcgg gcagaagccg gtcgcgcgcg cggcgacgga
     1981 gcgccgcctc cgcacgggtg atcgaaccat ggcggcgcgc ttcgtcggtc cagaccgaga
     2041 gcggcgccat cgcgtcgaaa tgccgatcgc gctcgatcag gaggccgaag ggaagtcgca
     2101 ccgcgcggat ttcgccaagc gagaaacagc cgagttcggg acagccgaag ccgaggtcgg
     2161 cgagaccgaa cagcgtatcg ccgtcctcgt cgatttcggt cgcgatccat gtcgcggcgc
     2221 cgacgggatt gaagagcttg acgacgggcc acggatcgaa ggcctcgccg cgcgcggcat
     2281 ggatgcggct cgcgacgcgg ttcgcgttga gcgcgaaggc gaggctgtag ggaagcaggc
     2341 tcattgccct tcccccttcg tcgccccgcg cgcttcggcg ggcgcatagg ccatgagata
     2401 atcggcggct ttcgacgcga ggctcgccgc cttgaagatc gcgcgcgtgt cggcgcgcag
     2461 gatcgcgagc cacgcgccga tatagtcggc gtggcgcacc gtcggccgga tgccgagcgc
     2521 ggcgcagagg aatgcgctcg cgagttcggc gcaaagttcc tcgcggccat aggccgcgct
     2581 tgcgaagccg cccgtctggt cgcggccaag gcgcgttggg tggccggtcc agtgaccgag
     2641 ctcgtgcagc gcggtgcgat aatagtcgat ctgttcgaag aacgcctgtt gcggcggcag
     2701 cgcgacataa tcgcccgacg gcgaataata ggcctcattg ccgccgatgc ggatatcggc
     2761 gccgctcgcc gcgagcagcg cctcggcgac cgggatggtc gcgcgc
//
LOCUS       DATMSU010000139         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_40507, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000139 DATMSU010000000
VERSION     DATMSU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40507"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..1021
                     /locus_tag="VN034_03720"
     CDS             257..1021
                     /locus_tag="VN034_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59738.1"
                     /translation="MDLGLAGKKVIMNGGAHGLGLASLKIFAAEGADVAFFSRDAEKV
                     AAAVAAIDAAGPGKVFGEQFDMTGNPDGYREWLAKARDALGGCDIFIHTASSSGQGAT
                     GDWQRGLDMDVMGAVHGVEVLTEALAASGSGSIIFMSSTAAVETFIVPQAFNALKAAL
                     ITYGSQLSQALAAQNIRVNIVSPGAIYYPGGNWEVIKSAVPALYEGTLAQMPMGRFGE
                     PEEVAKGIVFMASPACPYMTGAHLVIDGGFTKRVQF"
     gene            1032..1679
                     /locus_tag="VN034_03725"
     CDS             1032..1679
                     /locus_tag="VN034_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV59739.1"
                     /translation="MKLYQSLGPNPRVVLMYLAETGLTLDRQFVDIMAAENRRPDFYA
                     KSPLGHTPLLELDDGSHIVESIAICEYLAETHAANELLGATPEQRARTRMFVRIVDQL
                     VVVPMTAGFRGAEGLSMFKDRVLCLPDAAADLKRLAASGLEAVNNIIGAGPWIAGERF
                     SLADILLYSFVEFGAMVGQPPDPALTHLAAWRDRVAARTSAAASANPQQGIGEPA"
     gene            1676..2656
                     /locus_tag="VN034_03730"
     CDS             1676..2656
                     /locus_tag="VN034_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWV59740.1"
                     /translation="MTEPTIDRDKLRDIYTRTMRVARADEKFRSLLMTGKLAVIYYTV
                     RGQELVSAAAMAALEQDDYLVTTYRGQHDQIAKGVPLNPLFAEIAGKVGGTCRGKGGS
                     MHITHPETGVMVTTGVVGSGLPIANGLALASQNRDDGKVTMVCFGDGATNIGAFHEAM
                     NMAQLWKLPVIFLCQNNRYGEHTAFADHTKVDSIVTRARAYGMEGVKVDGNDAIAMYN
                     TTKAAVDRARAGEGPTLIEAMCYRMMGHFFGSDFSYMPKEHLAEMAAEDPLPRLRQLM
                     LDHQFSEAELDAIVADLDAQMDAAAEFAANSPLPGPEEIRKDVFEEEIAA"
     gene            2653..3645
                     /locus_tag="VN034_03735"
     CDS             2653..3645
                     /locus_tag="VN034_03735"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWV59741.1"
                     /translation="MSAQITMTQAINRAIDEAMAEDEGVILLGEDVAAKQGGGVFKIS
                     SGLTEKYGEKRIRATPISEQAIVGASVGAALAGFRPIAEIMLMNFVTVAMDQIVNHAA
                     KLRFMSGGQTNVPLVIRTTTGVGVGFGGQHSDMLEAWFAHVAGLKIVTPSNAADAQGL
                     MRAAIACNDPVIFIENILCYGLKSDDPGPGHVVPLGKAAVAREGSDCSIITYGRTVLD
                     ALEIAGKLAEEGISVEVIDLRTIAPYDEATVTASVEKTGRAVVLHEAVKQYGTGAEIA
                     SRLNEKLFGKLKAPVTRIGGAFSAVPMANALEQAWIPNKDAIADAVRAAVNWKG"
     gene            3650..3880
                     /locus_tag="VN034_03740"
     CDS             3650..3880
                     /locus_tag="VN034_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWV59742.1"
                     /translation="MAEELRIPKIGMSATEMTLNEWMVGDGERVEVGDIIYTVETDKT
                     TVEIEAQVAGTIRPTGVEGEVYPVGALVGTIE"
     gene            3877..4269
                     /locus_tag="VN034_03745"
     CDS             3877..4269
                     /locus_tag="VN034_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59743.1"
                     /translation="MTPLEASKAMYAAVARGEWDVVADFMAEDLVIHEPTSLPYGGEW
                     RGRDALQKLYAHVMGYWEEPVVKWMELVGGEKYAVALLHFTVTAKSSGKRFETHIAEV
                     TEFDDAGKMASMRIHYFDTAHMVEQLKA"
     gene            complement(4532..>4990)
                     /locus_tag="VN034_03750"
     CDS             complement(4532..>4990)
                     /locus_tag="VN034_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV59744.1"
                     /translation="GGLSLLLASDVRYAAPSFRCNAAYIRIGLGGCDMASSYFLPRLV
                     GASLASEMILTGRFIDAERALRAGLVSEIVEEAALLTRGLALADEMLATSPYGLRLSK
                     QALNLNIDAPSLEAAMAIEDRQQVILSATEDHREALAAFLEKRAPDYRER"
BASE COUNT          941 a         1548 c         1668 g          833 t
ORIGIN      
        1 gacccgatcc gcgcgaaagg catcatggcc agcggcgcat gacaaggccg catcacaggc
       61 cggacacatg gctgcaccaa ccagcaggtc gcatcacatc cagaaaccac ttgccaacgg
      121 agggccgtcc acacacggcc actcgtcgcc accggagcgg aaccttccgg atcaaagcgg
      181 gcgctcgcat ttttgctgat gtcccttggt ttctcgccac gatagtctcc ctgcgaaata
      241 gcaagaggag gatgcgatgg acctggggct ggccggcaaa aaagtgatca tgaacggtgg
      301 tgcacacggg ctgggcctcg cctcgctcaa gattttcgcc gccgagggcg ccgacgtcgc
      361 cttcttctca cgcgatgcgg agaaggtcgc ggccgccgtc gccgcgatcg acgctgcggg
      421 accgggcaag gtgttcggcg agcagttcga catgacgggc aaccccgatg gctatcgcga
      481 gtggctggcg aaggcgcgcg atgcgctcgg cggctgcgac atcttcatcc acaccgcaag
      541 ctcgtcgggg cagggcgcaa ccggcgactg gcagcgcggg ctcgacatgg acgtgatggg
      601 cgcggtgcac ggggtcgagg tgctgaccga ggcgcttgcc gcgtcgggtt cgggatctat
      661 cattttcatg tcgtcgaccg cggcggtcga gaccttcatc gtcccgcagg cgttcaacgc
      721 gctgaaggcc gcgctgatca cctatggctc gcaattgagc caggcgctcg cggcgcagaa
      781 tatccgcgtc aacatcgtct cgcccggcgc catctattat cccggcggca attgggaggt
      841 gatcaagtcg gcggttcccg cgctttacga gggcacgctt gcgcagatgc cgatggggcg
      901 tttcggcgag cccgaggagg ttgcgaaggg catcgtcttc atggcgtcgc ccgcctgtcc
      961 ctatatgacc ggcgcgcatc tcgtcatcga cggcggcttc accaaacgcg tccagttcta
     1021 ggcggcgggc gatgaagctc tatcaatcgc ttggccccaa tccgcgcgtg gtgctgatgt
     1081 acctcgccga aacgggactg acgctcgacc ggcagttcgt cgacatcatg gcggccgaga
     1141 accgccggcc cgatttctat gcgaaaagcc cgctcggcca tacgcccttg ctcgagctcg
     1201 acgatggcag ccatatcgtc gaaagcatcg cgatctgcga atatctggcc gaaacgcacg
     1261 cggcgaacga gcttctcggc gcgacgcccg aacaacgcgc gcggacccgg atgttcgtgc
     1321 gcatcgtcga tcagctcgtc gtcgtgccga tgaccgcggg ctttcgcggc gccgaaggct
     1381 tgtcgatgtt caaagaccgg gtgctgtgcc tgcccgatgc tgccgccgac ctgaagcggc
     1441 ttgcggcgag cgggctcgaa gccgtgaaca atatcatcgg cgccggacca tggatcgcgg
     1501 gcgagcgctt cagcctcgcc gacatcctcc tctattcctt cgtcgagttc ggcgcgatgg
     1561 tcggccagcc gcccgacccc gcgctcaccc atctcgccgc ctggcgcgat cgcgttgccg
     1621 cccgtaccag cgccgccgcc agcgccaacc cccaacaggg catcggagag cctgcatgac
     1681 cgaaccaacg atcgatcgcg acaaattgcg cgatatctac acccgcacga tgcgcgtcgc
     1741 gcgcgccgac gagaaattcc gctcgctgct gatgaccggc aagcttgccg tgatctatta
     1801 taccgtgcgc gggcaggaac tggtgtcggc ggcggcgatg gcggcgctcg aacaggacga
     1861 ttatctcgtc accacctatc gcggccagca cgaccagatc gcgaagggcg tgccgctcaa
     1921 tccgctgttc gcggagatcg cgggcaaggt cgggggcacc tgccgcggca agggcgggtc
     1981 gatgcacatc acccatcccg aaaccggggt gatggtgacc accggcgtcg tcgggtcggg
     2041 gctgccgatc gccaacggcc tcgcgctcgc gtcgcagaac cgcgacgacg ggaaggtgac
     2101 gatggtctgc ttcggcgacg gcgcgaccaa tatcggcgcc ttccacgagg ccatgaacat
     2161 ggcgcagctc tggaagctgc ccgtgatctt cctatgccag aacaaccggt acggcgagca
     2221 taccgccttt gccgaccata ccaaggtcga ttcgatcgtc acccgcgcca gggcctatgg
     2281 aatggagggc gtgaaggtcg acggcaacga cgcaatcgcg atgtacaaca ccaccaaggc
     2341 cgccgtcgac cgtgcgcggg cgggcgaggg gccgacgctg atcgaggcga tgtgttaccg
     2401 catgatgggg cattttttcg gctcggactt ctcctacatg ccgaaggagc atctcgccga
     2461 gatggccgcc gaagacccgc tgccgcggct ccgccagctg atgctcgacc accagttttc
     2521 ggaggccgag ctcgacgcga tcgtcgccga cctcgacgcg cagatggacg ccgccgccga
     2581 atttgccgcg aacagtccgc tgccggggcc agaggaaatc cgcaaggatg tcttcgaaga
     2641 ggagattgcg gcatgagcgc gcagatcacg atgacgcagg cgatcaaccg cgcgatcgac
     2701 gaggcgatgg ccgaggatga aggcgtgatc ctgctcggcg aggatgtcgc ggcaaagcag
     2761 ggtggcggcg tattcaaaat ctcgtcgggg ctgaccgaga aatatggcga gaagcgtatc
     2821 cgcgcgacgc cgatttcgga gcaggcgatc gtcggcgcga gcgtcggcgc ggcgcttgcg
     2881 ggcttccgcc cgatcgccga gatcatgctg atgaatttcg tgacggttgc gatggaccag
     2941 attgttaacc atgccgcgaa gctgcgcttc atgtcgggcg ggcagaccaa tgtgccgctg
     3001 gtgatccgca ccacgacggg cgtgggggtc ggtttcggcg ggcagcatag tgatatgctc
     3061 gaagcctggt tcgcgcatgt cgcgggattg aagatcgtca ccccgtcgaa cgccgccgac
     3121 gcgcaggggc tgatgcgtgc ggcgatcgcg tgcaacgacc cggtgatctt catcgagaat
     3181 atcctctgct acggcctcaa atccgacgat ccggggccgg ggcatgttgt cccgctcggc
     3241 aaggcggcgg tcgcgcgcga gggcagcgac tgttcgatca tcacctatgg ccgcaccgtg
     3301 ctcgacgcgc tcgaaatcgc gggcaagctt gccgaggagg gcatttcggt tgaggtgatc
     3361 gatctcagaa ccatcgcgcc gtacgatgag gcgacggtga ccgcatcggt cgaaaagacc
     3421 ggacgcgcgg tcgtgctgca cgaagcggtg aagcaatatg ggacgggtgc cgagatcgcc
     3481 tcgcggctca acgaaaagct gtttggcaag ctgaaggcgc ccgttacccg catcggcggc
     3541 gccttctccg cggtgccgat ggcaaacgcg ctcgagcagg cgtggatacc gaacaaggat
     3601 gcgatcgccg acgcggtgcg cgctgccgtg aactggaagg gctgaggcga tggcggagga
     3661 attgcgcatc ccgaagatcg gcatgtcggc gaccgaaatg acgctgaacg aatggatggt
     3721 cggcgacggc gagcgcgtcg aggtcggcga catcatctat acggtcgaga ccgacaagac
     3781 gacggtcgag atcgaggcgc aggtcgcggg cacgatccgg ccgaccggcg tcgagggtga
     3841 ggtctatccg gtcggcgcgc tcgtcgggac gatcgaatga cgccgctgga ggcgagcaag
     3901 gcgatgtacg ccgcggtcgc gcgcggcgag tgggatgtgg tcgccgattt catggccgag
     3961 gatctcgtca tccatgagcc gacttcgctg ccctacggcg gtgaatggcg cgggcgcgat
     4021 gcgctgcaga aactctacgc gcatgtcatg ggctattggg aagagccggt ggtcaaatgg
     4081 atggagctgg tcggcggcga gaaatatgcc gtcgctttgc tccatttcac cgtcaccgcg
     4141 aaatcgagcg gcaagcgctt cgaaacgcat atcgctgaag tcaccgaatt cgacgacgcg
     4201 ggcaagatgg cgtcgatgcg catccattat ttcgacaccg cgcacatggt cgagcaattg
     4261 aaggcctagg gtccgtactc aattagagca ccgtcgaggt ttgaagcaaa atggcccagt
     4321 gcgaggagag aaggcgcagg aatatgtcga tatttcaaga ctgctcgacg aagcaatggg
     4381 ccattttgat caaatccctt cgggacgccc aaatggcttc catctcggtc cgaaagccgc
     4441 ttggaaaggc aaccagcctt cccggcgcgt ctttcggccc gatctgttcg ccatttggtc
     4501 gcctcgacgg cgctgtaatt gagtacgggc cctaacgttc gcgatagtcg ggcgcgcgtt
     4561 tctcgaggaa ggcggcgagc gcctcgcgat ggtcctcggt cgccgaaagg atcacctgct
     4621 ggcggtcctc gatcgccatc gccgcctcga gactcggggc atcgatattg aggttcagcg
     4681 cctgtttaga gaggcggagg ccatagggcg aggtcgcgag catctcgtcg gcgagcgcga
     4741 ggccgcgggt cagcagcgcc gcttcctcca cgatctcgct gaccagcccc gcgcgcagcg
     4801 cgcgctcggc atcgatgaaa cgcccggtca ggatcatctc ggacgcgagg ctcgcgccga
     4861 cgaggcgcgg caggaaatag ctcgacgcca tgtcgcatcc gccgagaccg atccggatat
     4921 aggcggcgtt gcagcggaac gacggcgccg cgtaacgcac gtcggacgcg agcagcaacg
     4981 acaggccgcc
//
LOCUS       DATMSU010000140         2712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_134977,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000140 DATMSU010000000
VERSION     DATMSU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134977"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /locus_tag="VN034_03755"
     CDS             <1..1392
                     /locus_tag="VN034_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HWV59745.1"
                     /translation="IVEARDAPGGRAYHWKRDGFTFDAGPTVITDPPCLEELWALSGQ
                     EMGQDVDLIPVTPFYRLNWPDGTNFDYSNDEAALREEIAKLCPADVAGYDRFLGYSRG
                     VYEEGYVKLGAVAFLDFASMVRAAPALMKYRAWRSVYGVVSSYVRDERLRQALSFHTL
                     LVGGNPMTTSAIYALIHTIEKEGGVWFARGGTNALVAGMVRLFERLGGTLRLDDPVSR
                     IETESDRATGVTTQSGWHGEADMVATNADLMHSYRDLLGGHRRGNKVAKSLSRKRWSP
                     SLFVVHFGVKGEYPGVAHHSILFGPRYKGLLGDIYGGVIPDDFSLYLHHPSTTDPDMA
                     PPGHSTFYALAPVAHLGKAQADWDGDFGERYADAILEEVERRVAPGVRANLVTRFHYT
                     PADFGRDLSAHLGSAFSLEPLLRQSAYFRAHNRDDAISNLYFVGAGTHPGAGIPGVVG
                     SAKATARLMLEDL"
     gene            1389..1970
                     /locus_tag="VN034_03760"
     CDS             1389..1970
                     /locus_tag="VN034_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWV59746.1"
                     /translation="MKRLAVYCGSATPEDPIYIETARHVGRTLAKRGIGVVYGGGRLG
                     LMGAVADAALEAGGEVIGIIPDALVGAEVAHRGVTELHVVSGMHERKKMFTDLSDGFL
                     TIPGGVGTMDELWEAISWAQLGYHAKPVGLLNAAGFYNDLIAFNRNMIEVGFIRPAHA
                     GIMIVDAGLDELLDKMAAYVPHKTIFAMKAENL"
     gene            1970..>2712
                     /locus_tag="VN034_03765"
     CDS             1970..>2712
                     /locus_tag="VN034_03765"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HWV59747.1"
                     /translation="MTEEGAYDAHALYGFAHDSIARGSKSFALASQLFDRETRERVWL
                     LYAWCRAADDLTDGQDHGGAMKPNHDPGAAIAHIRAQTDKAFAGEPTGEAAFDALGFL
                     LTEVDIPRAVIEDIIAGFELDAKDWRPRSEDDLLRYSYHVAGAVGVAMALVMGIDPAD
                     ETTLDRASDLGIAFQLANIARDVAEDAAADRCYLPVEWMVELDIPPGQHMHPAFRGRL
                     SVMAKWLAEMAGEYEAAGRWGARRLPPRSR"
BASE COUNT          464 a          903 c          886 g          459 t
ORIGIN      
        1 atcgtcgagg cgcgcgacgc gcccggcggg cgggcctatc actggaagcg cgacggtttc
       61 accttcgacg cggggccgac cgtcatcacc gacccgccgt gccttgagga actatgggcg
      121 ctcagcgggc aggaaatggg gcaggacgtc gacctgatcc ccgtcacgcc tttctatcgg
      181 ctcaactggc ccgacggcac gaatttcgac tattcgaacg acgaagcggc gttgcgcgag
      241 gagatcgcga agctgtgtcc cgccgatgtc gcggggtacg accgctttct cggctattcg
      301 aggggcgtct atgaagaggg ttatgtgaag cttggcgcgg tcgcctttct cgacttcgcc
      361 tcgatggtcc gggcggcgcc ggcgctaatg aaatatcgcg cctggcgcag cgtctatggg
      421 gtcgtgtctt cctatgtgcg cgacgagcgg ctgcggcagg cgctatcctt tcatactttg
      481 ctcgtcggcg gcaatccgat gacgacgagc gccatctatg cgctgatcca cacgatcgag
      541 aaggaaggcg gcgtctggtt cgcgcgcggc ggcaccaatg cgctcgtcgc gggcatggta
      601 cgattgttcg agcgattggg cggcacgctg cgcctcgacg atccggtgtc gcggatcgag
      661 accgaaagcg accgcgcgac cggcgtgacg acgcaaagcg gctggcacgg cgaagccgac
      721 atggtcgcga ccaacgccga cctgatgcac agctaccgcg atttgctcgg cggtcatcgg
      781 cgcgggaaca aggtcgcaaa gagcctctcg cgcaaacgct ggtcgccctc gctcttcgtc
      841 gtccatttcg gggtgaaggg cgaatatccc ggcgtcgcgc accacagtat cctcttcggc
      901 ccgcgctaca aggggctgct gggcgacatt tacggcggcg tgatccccga cgacttctcg
      961 ctctatctcc accatcccag caccaccgac cccgacatgg cgccgccggg ccactcgacc
     1021 ttctacgcac tcgcccccgt cgcgcatctc ggcaaggcgc aggccgactg ggacggcgat
     1081 ttcggcgagc gatacgccga cgcgatcctc gaagaggtcg aacgccgcgt cgcgccgggc
     1141 gttcgcgcca atctcgtcac gcgcttccac tatacgcccg ccgatttcgg ccgcgacctt
     1201 tcggcgcatc tcggcagcgc gttcagcctc gagccgctgc tgcggcaaag cgcatatttc
     1261 cgggcgcaca atcgcgatga cgcgatttcg aatctttact tcgtcggcgc ggggacgcat
     1321 ccgggcgcgg gaatccccgg ggtcgtcggc agcgccaagg cgaccgcccg attgatgttg
     1381 gaagatttat gaaacgcctt gccgtctatt gcgggtccgc gacccccgaa gatccgatct
     1441 atatcgaaac cgcgcgccac gtcggacgca cgctggccaa acgagggatc ggagtcgtct
     1501 atggcggcgg ccggctgggg ctgatgggcg cggtcgccga cgccgcattg gaagccggcg
     1561 gcgaagtgat cggaatcatt cccgacgcgc tcgtgggcgc cgaggtcgcg caccgcggcg
     1621 tcaccgagct ccacgtcgtc agcggcatgc acgagcgcaa gaagatgttc accgatcttt
     1681 ccgacggctt cctcaccatc cccggcggcg tcggcacgat ggacgagctc tgggaagcga
     1741 tcagctgggc gcagctcggc tatcatgcga agcccgtcgg gctgctcaac gccgcgggct
     1801 tctataacga tctgatcgcg ttcaaccgca acatgatcga ggtcggcttc atccgccccg
     1861 cgcacgccgg gatcatgatc gtcgacgccg ggctcgacga actgctcgac aagatggcgg
     1921 cctatgtccc gcacaagacg atcttcgcga tgaaggccga aaatctctga tgaccgaaga
     1981 gggggcctat gacgcgcacg ccctctacgg tttcgcgcac gacagcatcg cgcgcggatc
     2041 gaaaagcttt gccttggcca gtcaactctt cgaccgcgaa acgcgcgagc gcgtctggct
     2101 gctctatgcc tggtgccgcg ccgccgacga tctgaccgac ggtcaggatc atggcggcgc
     2161 gatgaagccg aaccatgatc ccggcgcggc catcgcgcat atccgcgcgc agaccgacaa
     2221 ggcctttgcc ggcgagccga ccggagaggc cgccttcgac gcgctcggct tcctgctcac
     2281 cgaggtcgat atcccgcgcg cggtgatcga ggacatcatc gcggggttcg aactcgacgc
     2341 aaaggactgg cgcccgcgca gcgaggacga cctgctgcgt tatagctatc atgtcgccgg
     2401 cgcggtcggg gtcgcgatgg cgttggtgat gggcatcgac cccgccgacg agacgacgct
     2461 cgaccgcgca tcggatctcg gcatcgcctt ccagctcgcc aatatcgcgc gcgacgtcgc
     2521 cgaagatgcc gccgccgacc gctgctattt gccggtcgaa tggatggtcg aactcgacat
     2581 cccgccgggc cagcatatgc acccggcctt tcgcggacgc ctgtcggtga tggccaaatg
     2641 gctggccgaa atggccgggg aatatgaggc ggcgggccgc tggggcgcgc ggcggctgcc
     2701 cccgcgcagc cg
//
LOCUS       DATMSU010000141         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_11930, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000141 DATMSU010000000
VERSION     DATMSU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11930"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VN034_03770"
     CDS             complement(<1..475)
                     /locus_tag="VN034_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV59748.1"
                     /translation="MTSRETTHFLARPDIYDALPRPARERLEVIAFGAIQWPWLLRSL
                     WGGRRAEKAALLRRLDLPEDALPHLGSWKADTRFLTHIVDAIEELRPAVVVELGCGAS
                     SLVIAKALSMFGGGRLVSYDQHREFVVATTHWLASQGVSADLRPAPLGPPPGDWPG"
     gene            complement(570..1859)
                     /locus_tag="VN034_03775"
     CDS             complement(570..1859)
                     /locus_tag="VN034_03775"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWV59749.1"
                     /translation="MANVTVIGSQWGDEGKGKIVDWLASRADAVVRFQGGHNAGHTLV
                     VGEQVYKLSLLPSGIVTGTLSIIGNGVVLDPWALRDEITKLRGQGVAINPENFAIADN
                     CALILPFHRDLDALRETAAGAGKIGTTGRGIGPAYEDKVGRRAIRVCDLAHLDKLEPQ
                     LDRLTAHHDALRAGFGEPPIDRERLIADLKEIADYVLEYAQPVWKRLKKVRKAGARIL
                     FEGAQGVLLDIDHGTYPFVTSSNTVSGTAASGSGLGPSAVGFVLGIAKAYTTRVGSGP
                     FPTELEDETGQRLGERGHEFGTVTGRKRRCGWFDAVLVRQSCAVSGVTGIALTKLDVL
                     DGFDTIRICTGYRLRGKILDYFPAHAADQAEVEPIYEEMDGWHESTAGARSYADLPAQ
                     AIKYIQRVQELIETPIALVSTSPEREDTILIRDPFSD"
     gene            1989..2366
                     /locus_tag="VN034_03780"
     CDS             1989..2366
                     /locus_tag="VN034_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOPA 4,5-dioxygenase family protein"
                     /protein_id="HWV59750.1"
                     /translation="MINPDAPYHAHIYFDDAERAAAAALRDGFGRDPAILFVGDMTDG
                     AVGPHPIAQYEVHFLGAAVPAVVEAIEATELRALVHPLTDDDLADHTTLAHWIGEPIE
                     LDVTVLDPPGVNQGIARFGGSDF"
BASE COUNT          388 a          798 c          865 g          423 t
ORIGIN      
        1 ggcccggcca gtcgccgggc ggcggcccca gcggcgcggg gcgcagatcg gccgaaaccc
       61 cttggctcgc gagccagtgg gtcgtggcga caacgaattc ccgatgctgg tcataactga
      121 cgagccgccc gccgccgaac atcgacagcg ccttggcgat gacgaggctg ctcgccccgc
      181 agccgagttc gacgacgacc gccgggcgca attcctcgat cgcgtcgacg atatgcgtca
      241 gaaagcgcgt gtcggccttc cagctgccga ggtgcggcag cgcatcttcg ggaaggtcga
      301 ggcggcgaag cagcgcggcc ttttcggctc ttcgtccacc ccacagcgac cgcagcagcc
      361 acggccactg gatcgcgcca aaggcgatga cctccagccg ctcgcgcgcc gggcgcggca
      421 gggcgtcgta gatgtcggga cgcgcaagga agtgcgtggt ttcgcggctt gtcatccaca
      481 gccaactaag ggcggtggat ggcagttcag tgacgcgtcg ccggcttgcg tccggccgcc
      541 gcgcccgtgc gacgaccggc gtcttcggct cagtcgctga acggatcgcg gatcaggatc
      601 gtgtcttcgc gttcggggct cgtcgacacg agtgcgatcg gggtttcgat cagttcctgc
      661 acgcgctgga tatatttgat cgcctgcgcg ggaaggtcgg catagctgcg cgcgcccgcg
      721 gtcgattcgt gccagccgtc catttcctcg tagatcggct cgacctcggc ctggtcggcg
      781 gcgtgcgcgg ggaaatagtc gaggatcttg ccgcgcagcc ggtagcccgt gcagatgcgg
      841 atcgtgtcga agccgtcgag cacatcgagc ttggtgagcg cgatgccggt gacgcccgat
      901 accgcgcagc tctggcggac gagcaccgca tcgaaccagc cgcagcgacg cttgcgcccg
      961 gtgacggtgc cgaattcatg cccgcgttcg ccgagccgct ggccggtctc gtcctcgagc
     1021 tcggtcggga aggggccgct gccgacgcgc gtcgtatacg ccttcgcgat gccgagcacg
     1081 aagccgacgg ccgaagggcc gaggcccgat cccgacgcgg cggtgccgct caccgtgttc
     1141 gagctggtga cgaagggata ggtgccgtgg tcgatgtcga gcaacacgcc ctgcgcgcct
     1201 tcgaacagga tgcgcgcgcc ggccttgcgg accttcttca ggcgcttcca gacgggctgg
     1261 gcatattcga gcacatagtc ggcgatttct ttgagatcgg cgatcaggcg ctcgcggtcg
     1321 atcgggggct caccgaaacc cgcgcgcagc gcgtcgtggt gcgcggtcag acggtcgagc
     1381 tggggttcga gcttgtcgag gtgcgcgagg tcgcagacgc ggatcgcgcg gcggccgacc
     1441 ttgtcctcat aagccgggcc gatgccgcgt ccggtcgtcc cgatcttgcc cgcgcccgcg
     1501 gcggtttcgc gcagcgcgtc gaggtcgcgg tggaagggaa ggatcagcgc gcaattgtcg
     1561 gcgatcgcga aattctcggg attgatcgcg acgccctggc cgcgcagctt ggtgatctcg
     1621 tcgcgcagcg cccacggatc gagcacgacg ccgttgccga tgatcgacag cgtaccggtg
     1681 acgatgcccg agggaagcag cgacagcttg tagacctgct cgccgacgac gagcgtatgg
     1741 ccggcgttgt gaccgccctg gaagcggacc acggcgtcgg cgcggctggc gagccagtcg
     1801 acgatcttgc ccttgccctc gtcgccccat tgcgacccga tgacggtaac atttgccatg
     1861 atgctgtcct gctgagcggg cgccaaaaaa gcccggcgcg gccttagcgg atttggccgc
     1921 agaggcaagg gtggaggtgc tggcctttca agtcccgtcc ctttagggcg agggcgcagg
     1981 agataccgat gatcaacccc gatgcccctt atcatgccca tatctatttt gacgatgcgg
     2041 agcgtgccgc cgccgcagcg ctacgcgatg gcttcggccg cgatccggcg atattgtttg
     2101 tcggcgatat gaccgacggc gcggttgggc cgcatccgat cgcgcaatat gaggtgcatt
     2161 ttctgggcgc agcggtgccg gcggtcgtcg aggcgatcga ggcgacggaa ctgcgcgcgc
     2221 tggtccatcc gctgaccgat gacgatctcg ccgatcatac gacgctggcg cactggattg
     2281 gcgagcccat cgagctcgac gtgaccgtgc tcgatccgcc gggggtcaat cagggcatag
     2341 cgcggtttgg cgggtcggat ttctagacga tcaaaatatt ggcagtttag acttcgaccg
     2401 gcccgtcggg cccaagccgc cagccgcagc cgagcgcgcg cgcatcgtcg ctttcggata
     2461 gcgccgccac cgtc
//
LOCUS       DATMSU010000142         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_59536, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000142 DATMSU010000000
VERSION     DATMSU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59536"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1123)
                     /locus_tag="VN034_03785"
     CDS             complement(71..1123)
                     /locus_tag="VN034_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWV59751.1"
                     /translation="MFDHPILPTTIVGSYPQPDWLIDRERLKASLPPRVRAETLWRVP
                     EPWLADAQEAATLMAIRDQEELGIDIVGDGEMRRESYSNRLATALSGIDREKHGTAID
                     RTGNANPVPLVSGPIRRVAPIEAQDAAFLRRHSTKPVKLTLPGPFTMTQQAENGYYPD
                     ARALAMDYADAVNAEVKDLFAAGVDVVQLDEPYLQARAAEANSYAIEAINRALDGVGG
                     TTALHICFGYAMVHHGAGATGPKPKAYDFLAELEASAIDVISIEAAQPGLDPAILAEL
                     PTKTIMYGVLDLSVPEVETPEVVAGRIREALRYVDAERLWIAPDCGMKYHSREHSQAK
                     LKAMVDGTALVRAELK"
     gene            1210..2775
                     /locus_tag="VN034_03790"
     CDS             1210..2775
                     /locus_tag="VN034_03790"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HWV59752.1"
                     /translation="MIDLEAIRTLADIPAAQAKMRGQATAVKFGDCETSFAQLDARSN
                     RVAHALAASGIVPGDRVSALTKNHDSWYPLFFGTARARACFAPINCRLAPAEIGFILG
                     DAGPKLLFVGEDFFDCALAAVADLASPPRLIALYGEHPAFEPFDAWLGEASDAPLADA
                     PQLADDVLQLYTSGTTGLPKGVVLTNANYRTFLEAATEVDGFAYGEDETVMIVMPLFH
                     VAGTNVSFSGLAQGGRLVLVKDFTAPDAVRMMREENVAHAFLAPAMIQMMLLQPDANA
                     GAYPQLKSIAYGASPIAEDVLRRARATFGCDFVQFYGMTESAGGGSYLSPAAHDLPGK
                     LTSCGKPWPGAAMAILDGAGKELGDGEIGEIAIRGGIVMKQYWNRAAATEETLAGGWL
                     HTGDVGYRDADGFYYVHDRIKDMIVSGGENVYPAEVESAIMGCPGVADVAVIGVPDDK
                     WGEGVKALIVPAAGATLDPADVIGWARERIAAYKVPKSIEFIDALPRNPSGKVLRREL
                     RAPYWEGRDRAVG"
BASE COUNT          450 a          919 c          936 g          507 t
ORIGIN      
        1 gcgaaagcgg gaacccagcg agcgaacgtc gcaactgggt tcccgctttc gcgggaatga
       61 cgacgttagc ttatttcagc tcagcccgca ccagcgcagt cccatcgacc atcgccttga
      121 gcttcgcctg gctatgctcg cggctgtggt atttcatccc gcagtcgggc gcgatccaca
      181 gccgctcggc gtcgacgtag cgcagcgcct cgcgaatgcg tcccgcgacc acctccgggg
      241 tctcgacctc gggcaccgac aggtcgagca caccatacat gatcgtcttg gtcggcagtt
      301 cggctaggat cgcggggtcg agccccggct gcgccgcctc gatcgaaatc acgtcgatcg
      361 ccgaggcttc gagttcggcg aggaagtcat aggctttggg cttcggcccc gtcgcgcccg
      421 cgccgtgatg caccatcgca tagccgaagc agatgtgaag cgcggtcgta ccaccgacgc
      481 cgtcgagcgc gcggttgatc gcctcgatcg catagctgtt cgcctcggcg gcgcgcgcct
      541 gcagataggg ttcgtcgagc tggacgacat cgaccccggc cgcgaacagg tccttcacct
      601 cggcattgac cgcgtcggca tagtccatcg cgagcgcgcg cgcgtcggga taatagccat
      661 tctcggcctg ctgcgtcatc gtgaacgggc cgggcagggt gagcttcacc ggcttcgtcg
      721 aatggcggcg caggaaggcc gcatcctgcg cctcgatcgg cgcgacgcgg cggatcgggc
      781 ccgacacgag cggtaccggg ttcgcgtttc cggtgcggtc gatagcggtg ccgtgcttct
      841 cgcggtcgat ccccgacagc gcggttgcaa gccggttcga atagctttcg cggcgcatct
      901 cgccgtcgcc gacgatgtcg attccgagct cctcctgatc gcggatcgcc atcaaggtcg
      961 cggcctcctg cgcgtcggcg agccagggtt cggggacgcg ccacaaagtc tcggcgcgca
     1021 cgcgcggggg caggctggcc ttcagccgct cgcggtcgat cagccagtca ggctgggggt
     1081 agcttccgac gatcgtcgtc ggcaatatgg ggtggtcgaa caaggccaat cctctcgatt
     1141 tttgggctgg tcaaacattt gcttagtatt ctatctaatt caagtcgaaa cgacggttgg
     1201 gagagcaaaa tgatcgatct ggaggccatc cggacgctgg cggacattcc ggcggcacag
     1261 gcgaaaatgc gggggcaggc gaccgcggtg aaattcgggg actgcgagac gagcttcgcg
     1321 cagctcgatg cgcgctcgaa tcgcgtcgcg cacgcgctgg ccgcttcggg cattgtgccc
     1381 ggcgaccgcg tatcggcgct gaccaagaac cacgacagct ggtatccgct cttcttcggc
     1441 accgcgcgtg cgcgcgcctg ttttgcgccg atcaactgcc gcctcgctcc cgccgagatc
     1501 ggcttcatcc tcggcgatgc cgggcccaag ctgctgttcg tcggcgagga tttcttcgac
     1561 tgcgcgctcg ccgcggtcgc cgatctcgct tcgccgccgc gattgatcgc gctctatggc
     1621 gaacatcccg cgttcgagcc gttcgatgcg tggcttggcg aggcgtccga cgcgccgctc
     1681 gccgacgcgc cgcagctcgc cgacgatgtg ctccagctct acaccagcgg cacgaccggc
     1741 ctgcccaagg gcgtcgtgct caccaacgcc aattatcgca ccttcctcga ggctgcgacc
     1801 gaagtcgacg gcttcgccta tggcgaagac gagacggtga tgatcgtcat gccgctgttc
     1861 cacgtcgcgg ggacgaacgt cagcttttca gggcttgcgc agggcggccg gctggtgctg
     1921 gtcaaggatt tcaccgcccc cgacgcggtg cggatgatgc gcgaggagaa tgtcgcccac
     1981 gcctttcttg cccccgcgat gatccagatg atgttgctcc agcccgatgc gaatgccggt
     2041 gcctatccgc aactcaagtc gattgcttat ggcgcgtcgc cgatcgccga ggatgtgctg
     2101 cgccgcgcgc gcgcgacctt cggctgcgat ttcgtgcaat tctacggcat gaccgagtcc
     2161 gccggcggcg gctcctatct ctcgcccgcc gcgcacgatc tgccgggcaa gctcacatca
     2221 tgcggcaagc cgtggccggg cgccgcgatg gcgatcttgg acggtgcggg caaggaactc
     2281 ggcgatggcg agatcggcga gatcgcgatc cgcggcggca tcgtgatgaa gcaatattgg
     2341 aaccgcgccg ccgcgaccga agagacgctc gcgggcggct ggcttcacac cggcgacgtg
     2401 ggttaccgcg acgccgacgg cttttactat gttcacgacc ggatcaaaga catgatcgta
     2461 tcggggggcg agaatgtcta tccggccgag gtcgagagcg cgatcatggg ttgccccggc
     2521 gtcgccgacg tcgcggtgat cggcgtcccc gacgacaaat ggggcgaagg cgtgaaggcg
     2581 ctgatcgtgc cggcggctgg cgcgacgctc gaccccgccg acgtcatcgg ctgggcgcgc
     2641 gagcgcatcg ccgcctacaa ggtgcccaaa agcatcgaat tcatcgacgc cttgccgcgc
     2701 aatccgtcgg gcaaggtgct ccgccgcgaa ttgcgcgcgc catattggga aggtcgcgac
     2761 cgcgcggtgg ggtaggctgg acttcggctt tggggtggat ttaagccatt cc
//
LOCUS       DATMSU010000143         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_97596, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000143 DATMSU010000000
VERSION     DATMSU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97596"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..>2192)
                     /locus_tag="VN034_03795"
     CDS             complement(59..>2192)
                     /locus_tag="VN034_03795"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HWV59753.1"
                     /translation="WTPVTGEPGSGWGYTYDGEKISGIKQTHQPSPWMNDYAAFALMA
                     ETGPVKVKQQERGSWFSHKAEVARPYSYKVYLADYDVTAEVAPTMRAAQFRFTFPESD
                     DSHILLDGLAGGSMVKVDVKNRRITGYVRNNHGGVPDNFHNYFVAEFDRDFTLARTWA
                     GENAPTNDLAREGDHVGAVLSFKTRKDDKVHVRVASSFISPEQALLNLRTEIGGDSFD
                     TTKAKAKAEWEKELSRIRVTDPDAEKIRTFYSALYRMLQFPRTFYENDANGKIIHYSP
                     YDGKVHDGYMFTDNGFWDTWRAVFPFFALMYPERDSQFMQGLVNTYKQGGWLPEWASP
                     GYRNVMIGSNSANLIADAYFNGVRGFDVETLYEAMIKNATTSEGRPRDKAGKVIAAVG
                     REGAEYYNRLGYVPYDVGINENAARTLEYATADFSIAQLAKLLGKDADAKKYMERALN
                     YQKLYDNQSGWIRGRNQDGSWSTPFNPYKWGDAFTEGNALHYSWAVMQDVQGLMNLMG
                     GRQKFVERLDAIFTTPPIFDESYYGQVIHEIREMQIVDMGQYAHGNQPIQHMPYLYNW
                     AGAPWKTQHHVRDIMAKLYSSAPDGYPGDEDNGQTSAWYVFSALGFYPVASATGEYAI
                     GSPLFQKAELTMPNGKTLTINAKNNSDANIYLQSVAVGGKPLTKTYFTHRALSQGGTV
                     DFVMGAKPNKQWGAAPADAPFSISAPSK"
BASE COUNT          362 a          666 c          689 g          475 t
ORIGIN      
        1 agcgcgccgg cgccgaccat gatttcgcgg cggcctgggg tcatgttcat ctccgagttc
       61 atttggaagg cgcgctgatc gagaagggcg cgtcggcggg ggcggcgccc cattgcttgt
      121 tgggcttggc gcccatcacg aagtcgaccg tcccaccctg cgacagcgcg cggtgggtga
      181 aataggtctt ggtcagcggc ttgccgccga ccgccaccga ttgaagatag atattcgcgt
      241 cgctgttgtt cttcgcgttg atcgtcagcg tcttgccatt gggcatggtc agttccgcct
      301 tctggaacag cgggctgccg atcgcatatt cgcccgtcgc cgaggcgacc ggatagaagc
      361 ccagcgccga gaagacgtac caggccgagg tctgaccatt atcctcgtcg cccggataac
      421 cgtcgggcgc cgacgaatag agtttcgcca tgatgtcgcg cacatgatgc tgcgtcttcc
      481 acggcgcacc cgcccaatta tagagatagg gcatgtgctg gatcggctgg ttgccatggg
      541 catactgccc catgtcgacg atctgcatct cgcgaatctc gtggatcacc tgcccgtaat
      601 agctttcgtc gaagatcggc ggggtggtga agatcgcgtc gagccgctca acgaatttct
      661 gccgcccgcc catcaggttc atcaacccct gaacatcctg catcaccgcc cagctgtaat
      721 ggagcgcatt gccctcggtg aaggcgtcgc cccatttgta ggggttgaac ggcgtcgacc
      781 acgagccgtc ctgattgcgg ccgcggatcc agccgctctg attgtcgtag agcttctggt
      841 agttcagcgc gcgctccata tatttcttcg catcggcatc cttgccgagc agtttcgcga
      901 gctgggcgat cgagaagtcg gcggtcgcat attcgagcgt gcgcgcggca ttttcgttga
      961 tgccgacgtc gtatgggaca tagcccagac ggttgtaata ttcggcgccc tcgcgcccga
     1021 ccgcggcgat gaccttgccc gctttgtcgc gcgggcggcc ttcgctcgtc gtcgcattct
     1081 tgatcatcgc ttcatagagc gtctcgacgt cgaagccgcg cacgccgttg aaataggcgt
     1141 cggcgatcag gttcgccgag ttcgacccga tcatcacatt gcgatagccg gggctcgccc
     1201 attcgggcag ccagccgccc tgcttgtagg tgttgaccag cccctgcatg aactggctgt
     1261 cgcgttcggg atacatcagc gcgaagaagg ggaaaaccgc gcgccacgtg tcccagaaac
     1321 cattgtcggt gaacatatag ccgtcgtgca ccttgccgtc gtacgggctg tagtggatga
     1381 tctttccgtt cgcgtcattt tcatagaagg tgcgcgggaa ctgcagcatc cggtagagcg
     1441 ccgaatagaa ggtgcggatc ttttcggcat cgggatcggt cacgcggatg cgcgagagtt
     1501 ccttctccca ctccgccttc gccttcgcct ttgtcgtgtc gaagctgtcg cccccgattt
     1561 cggtgcgcag gttcagcagc gcctgttcgg ggctgatgaa ggaggaggcg acgcggacgt
     1621 ggaccttgtc gtccttcctg gtcttgaagc tgagcaccgc gccgacatga tcgccttcgc
     1681 gggcgaggtc gttcgtcggc gcgttctcgc ccgcccaggt gcgcgcaagc gtgaaatcgc
     1741 ggtcgaattc tgcgacgaaa taattgtgga aattgtcggg cacgccgccg tggttgttgc
     1801 ggacatagcc ggtgatgcgg cggttcttga cgtcgacctt gaccatcgag ccgcccgcga
     1861 gcccgtcgag caggatgtgc gaatcgtcgc tttcggggaa ggtgaagcgg aactgcgcgg
     1921 cgcgcatcgt cggtgcgacc tctgcggtca cgtcatagtc ggcgagatag actttgtacg
     1981 aatagggacg cgcaacctcg gccttgtggc tgaaccagct gccgcgttcc tgctgcttga
     2041 ccttgaccgg cccggtttcc gccatcagcg cgaaggcggc ataatcgttc atccacgggc
     2101 tcggctggtg cgtctgcttg atgccgctga tcttctcgcc atcataggta tagccccagc
     2161 cgctgccggg ctcgccggtc accggggtcc ag
//
LOCUS       DATMSU010000144        15245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_135055,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000144 DATMSU010000000
VERSION     DATMSU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 15245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15245
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135055"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1438
                     /locus_tag="VN034_03800"
     CDS             83..1438
                     /locus_tag="VN034_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59754.1"
                     /translation="MEKPVTTHAPGHPPPFPEPSPLHRAVGASALGNAVEWFDYGIYA
                     YGVTYISAALFPGDMEDAVLFALATFAISFLVRPLGGLFWGPLGDRIGRKSVLAMTIL
                     LMAGATFAVGLIPSYDSIGFWAPTLLILLRMLQGFSTGGEYGGAATFMAEYAPDERRG
                     FYGSFLEFGTLAGFSFGAALMLGYSLHLGDQAMHEWGWRIPFLIAGPIGLIGMYVRSK
                     MEDTPIFREEMASAERRNPPGIAIMLRDYWRPLLVVGGLVVALNVVNYSLLSYMPTYL
                     QRRIGLSNEEALLVPIIGMLFMMVFLPFAGALSDRVGRRAMWRWSLIGLLLGVVPLYM
                     LIGTGFTGALIGFMALGLLYVPQLATISATFPAMFPTAVRFAGFAIAYNISTSIFGGT
                     APMIGSGLITLTGDPLMPAYYMMLACLAGLAALRFMPETAGRSLREDPFARKTALASD
                     T"
     gene            1562..1846
                     /locus_tag="VN034_03805"
     CDS             1562..1846
                     /locus_tag="VN034_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HWV59755.1"
                     /translation="MLEDMPGRPAAVMGPFGPLTPKDLPSSDTTYWVSRRKAEVLAAI
                     NGGMLTIAEACARYRLSREELDAWQRSIDRAGIPGLRITRLQQYRGRRME"
     gene            complement(1955..2257)
                     /locus_tag="VN034_03810"
     CDS             complement(1955..2257)
                     /locus_tag="VN034_03810"
                     /inference="COORDINATES: protein motif:HMM:NF019501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BA14K family protein"
                     /protein_id="HWV59756.1"
                     /translation="MSGKSIAIVAAVAISLSATVVSAPPASAQYLDSYLSSQRDNNRI
                     RHQQKIRQERLAQQAGNRKGASWQRHVAACKRAYRSYDPKTDRYTVRRGVTRKCQL"
     gene            2531..3121
                     /locus_tag="VN034_03815"
     CDS             2531..3121
                     /locus_tag="VN034_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV59757.1"
                     /translation="MRENGDIIAWIAREIIPHEGAIRKWLARRWGHVIDVEDVIQDAY
                     CRLANLASVEHIDNPGGYFHRTASAVVTDHMRRAGIINFTSMTQIEWSNVIDDDPLPD
                     RSLETRQEYERVAGLLAMLSDTGRKVIELRRIEGLSRKETAERLGVSENDVKNHLVRG
                     LQKILGAMAEQDARSKGDAPADTGKKDEVFGKRRPY"
     gene            3090..4136
                     /locus_tag="VN034_03820"
     CDS             3090..4136
                     /locus_tag="VN034_03820"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWV59758.1"
                     /translation="MKCLASAGPINAIDEQAAVWAVETAHGEMNAQSRADLEAWLSAD
                     TRHRGAYIRACAWLRAAEHVATAAHRAPAASVPIVPLRTSDNDNDGDNEGIAPGALRM
                     LASRWSGRAVAGGAAVAMSLALLVAVGVPVPGLVTPTETSASAKIVTLKDGSVATLGR
                     DAQIQVSLSEGIRKITLLRGEATFEVAKDKSRPFVVRSGDVYAQATGTVYSVRRVGLT
                     GGGVRVSEGSVLVWPRDERDQAVLLHAGGTVTLDPGPLATTAQAPRPASRRLPPPELA
                     QISLDNVPVRAAVARFNRVNSTKIILADPAIGDIEIIGLYRANDPEHFAEAVAAISGA
                     HIERSGSTIVIKMK"
     gene            4232..7342
                     /locus_tag="VN034_03825"
     CDS             4232..7342
                     /locus_tag="VN034_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59759.1"
                     /translation="MRSYYIRSAASATAVAAAMIFPAAAQAQVRSFDVPTQSASNGIR
                     EFARQAGIQVTLAGRDGDDRTTNAVRGSFEIRDGLARLLADTGLTVRSFDGKVAILAA
                     SAADVADADETITVTGSRIARPELESAMPIGIVSMEAGRKAGNTTVYETVLRDVAVGP
                     GNGPYNSAPEGQYDGGMATIELRNMGVSRSLTLIDGRRRVSGAASSSAVDINMIPSGM
                     VERIEIVTGGAAAIYGADAVTGAANIITRKDIDGLEVSATTGFTQDGGGSKSQLSFVA
                     GTRFSDDRGSITIGGTWMKNNPIYFHQRYSRNTNISYQANPENTGPDDGIPDRVIRYN
                     MTNMYLQPNANIYVGGNTYLLAPDGSAYVGQYANGCISGCGTSRDGGDGGFPQSQYWS
                     DFLIAPIENYAFIGRFDYALTDGVNYEFRVDYGRSRYDAYRRPYRDDDRLTWLNGAGG
                     ATARLDNPYLPDSFRQIMLANDLTSTAVRRSYENFGQMTDYSDRQSLTVSTGFSGELA
                     DRFKWEVFGQYGRSTNDIHADNVPIASRLIAARDVISDPVSGAPVCRDAAARANGCVP
                     FNIFSEAPLTAEQRGWMMATRRKHREQTQKIFGASITGSPFALPAGEVQAVLGAEHRR
                     EAIHNVDDNGAAPPEKELSHLGAWAPYEPELRASNHVSEVYGELVVPVLRDLPFIHKL
                     TVEGAVRYSDYSDFESTTTWKAGATWMPFSGLTLRAVRSRSVRVPNFGERYSARAVQE
                     IGVRDACLETSYQANPTRKANCAALMQQLGIPTPYLPSEIGTGYVITGGSMDVTPETS
                     NSLTAGLVWQPGFLSGFDLTVDYWNIKIGNMIAAIGRQDVLNLCMDLPSIDNQFCDRI
                     TRDSRGYAEGVDTSYMNVSESEAEGIDVGANYRTDVGPGRLAVAVRASYLIKFETTTL
                     PGVETSRIIYDGGYQNPRVRANMFASYSIGDWEVGLNTRLWGSAVNYTNVSDEAYERN
                     KLPAKVYNDLNVGWNLNENATLRVGINNLLDVRPSYRPRTYYQGGGGVYDVYGRYFFS
                     SVNFKF"
     gene            7354..8271
                     /locus_tag="VN034_03830"
     CDS             7354..8271
                     /locus_tag="VN034_03830"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWV59760.1"
                     /translation="MIKSLHLRRCAVLAAAVTSLSSPVAFAEEAPAAASIAQPRPIAV
                     IAHRGGALLRPENTMPAFRHAAEIRAEYLEFDMEMTADDRIAVYHDALINPDFCKQDD
                     GSPAPAQPVRKLGLAAIQKLDCGTGVRPQYAGPRHVAVTGARVPELGEVLHAFKGSEA
                     LFFAETKIPKDADIDPVEFARLVEAAVRENGLEDRLILQSFDFRTIDALHEINPRIRT
                     CLLGVPRLTRDYLAMLRRHNSKCIVLGIGEVDRAGTARLQEAGILVFSGVADREEEWR
                     QYAELGVDALFTNDPEGAIAYLERSGLRN"
     gene            8343..9917
                     /locus_tag="VN034_03835"
     CDS             8343..9917
                     /locus_tag="VN034_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HWV59761.1"
                     /translation="MAFAKTLDRRNLIKAAGSLAVLAGFSRAPLFARPLGANPFTLGV
                     ASGDPWPDGFVIWTRLAPRPFDEHAGMPAARVPVRWEVAEDAACTRIVQSGETLAMPE
                     LGHSVHVEVAGLRPHRPYWYRFAVAGSDVSPIGTARTAPASDAIVDRVRIGVAGCQHY
                     EVGHYEAWAHLANEPDLDLIFHYGDYVYEGGTTPLGPQASGVPIVRQHIGGEIYSIED
                     YRRRYAQYKGDPALQAAHAACAFACSFDDHEIDNNWAGDFDQDGTSPEIFALRRYAAL
                     QAWYENMPVRKALFPAQGGLTAYRRLDYGRLLRTHVLDTRTNRSDQVCNDGKIRPCPP
                     DRLQSPEILGRVQEAWLDEGLGNDAVWNLLAQQIIVMPVDFRADDAAEARFATDLWDG
                     YRPARERLIESIRKHELTNVVIATGDHHKHLVGSVPEDDDRPDGPKVAVEFQAASISS
                     NGNGKGEAGLEQMMRNNPHFDLYTDRRGYQLFEITPKEWRTEVKVMDQVERPGGTIGT
                     LARYAVSPDAPILHRE"
     gene            10113..10607
                     /locus_tag="VN034_03840"
     CDS             10113..10607
                     /locus_tag="VN034_03840"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HWV59762.1"
                     /translation="MAVAALGAALALGGCGKSDDKAAAAGTVKREAGNWKTDIKLVKF
                     EMPGVPDNVKDQMSKQLAAASGTEQCLTQEQADKEDVAGALSKGYGEACTWSKNQIGD
                     GKIDVAGTCTQGAQKIELALAGTLGAKKADVLVTSKGKSPMGGGAMEMQMQVTSTNIG
                     PCKG"
     gene            complement(10811..10886)
                     /locus_tag="VN034_03845"
     tRNA            complement(10811..10886)
                     /locus_tag="VN034_03845"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10851..10853),aa:Trp,seq:cca)
     gene            10979..11926
                     /locus_tag="VN034_03850"
     CDS             10979..11926
                     /locus_tag="VN034_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWV59763.1"
                     /translation="MSDASSPALPIYGPPQHYLGGIALRLLAMVSLSLMFVLVKKIDA
                     AGIHVVESLFWRQALVLPFLLAWVMATSGFSSLKTRRIGAHARRMLMGLTGMVCNFGA
                     MILLPMAEATTISLSVPIFAVIFAAILLGEATGWQRWSAVIVGFVGVLVVLDPLASFA
                     GGFGGTHGAGTLVALTGAIMTALITIAVRDLGRTENAATIVFWFSLLSMIPLGIALPF
                     VITPHDGHEWLLLIGLGFLGAVVQMSLTGALRLAPVSVVIPMDYSSLLWAIAAGWFFF
                     GTLPADTTWVGAPLIIASGLFIAWREHRRHIERPKEVAA"
     gene            11923..12546
                     /locus_tag="VN034_03855"
     CDS             11923..12546
                     /locus_tag="VN034_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWV59764.1"
                     /translation="MTLPILYHCPDARSLRCLWAVEEAGIDVDLKLLKFPPRAFEPDY
                     RAVNPLMTIPGWVEDGRLMTESAAICERIAEGTPLEVRRDEADYWAWRNWLHRSDATL
                     TFPLAIMIRYTRVEPEERRLAQAVEDYKAFFGGRAKSIKAELADGREWLVAGRFTIAD
                     IAIGYAAFLATTLGAGDVLGDATRAWLDRCMAREGFRRARERQKAAA"
     gene            complement(12703..13227)
                     /locus_tag="VN034_03860"
     CDS             complement(12703..13227)
                     /locus_tag="VN034_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59765.1"
                     /translation="MNGRRLALRVALVAILSALLGASATVWRLSGVQAAQERSHRIAQ
                     ARLRCLMYWQYRSGIADADFEFRSGRKGLLQAVESHDPVYKRWTPGLHDDATAEVRND
                     YPVAGLTLDDLDLSQVGSIGPLCDGETRYAWDYNITMLRLLGRDRDAQPPYLSPELSK
                     PPRQVSKKAVARPQ"
     gene            complement(13224..14783)
                     /locus_tag="VN034_03865"
     CDS             complement(13224..14783)
                     /locus_tag="VN034_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HWV59766.1"
                     /translation="MGRDVSELFTFDEFLTHWAAERPGRVAMREEDRVYSYAQLDDRT
                     ARAASALIAAGLKKGDRIAWIGKNSDLYFTLFYGAARAGIVMAPIGWRLSPAEWAFIV
                     NDTQAKIVFAGPGFEGLADQLAGKLANDPAIMSAADAWAMIEGAARAPFEPSGANDAV
                     LQLYTSGTTGNPKGAVLSNHNLFALRKHSSTLDLPYTKWEDDEAVLVAMPCAHIGGTG
                     LGIMALASGLPGVILAEFNPDGVFDAVEQHGVTRFFIVPAALQMLLMHPRCASVDYSR
                     LKYILYGAAPIPLELLRQCIKMFGAQFIQAYGMTETTGTISMLPPEDHDPAGNARMRS
                     AGKALPGVEIVILGPDGQPVPTGEVGEVVTRSSNNMIGYWNLPDATAKTMTDDGWIRT
                     GDAGYLDADGYLFIHDRMKDMIITGGENVYPAEVESAIFGHPAVQEVAVIGIPDTKWG
                     ETVKAVVVAKPGTTADEADIIAWARERIAPFKCPRSIDVIEALPRNASGKILRKDLRA
                     PYWEGYERMVN"
     gene            complement(14887..>15245)
                     /locus_tag="VN034_03870"
     CDS             complement(14887..>15245)
                     /locus_tag="VN034_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HWV59767.1"
                     /translation="PLALDASASGWPVDRADAILCINMAHISPWQATLGLFAGAARLL
                     APGAPLILYGPYIEPDVPTAASNLDFDANLRARDPAWGLRDTDRVKSAARDAGLAFAE
                     RRAMPANNLMLLFRRT"
BASE COUNT         2783 a         4932 c         4791 g         2739 t
ORIGIN      
        1 cgcgcacgac gccggtcgcc gcgctgctcg accccgccgg ccccgagcaa tcgaccgaaa
       61 accatccgca aacaggtgac ccatggaaaa gcccgtaacg acccatgccc cggggcaccc
      121 gcccccgttt cccgaaccct cgccgctgca ccgcgcggtc ggcgcctcgg cgctgggcaa
      181 tgcggtcgaa tggttcgatt atggcattta tgcctatggc gtgacctata tctcggcggc
      241 gctgtttccc ggcgatatgg aagatgcggt gctgttcgcg ctcgcgacct ttgccatttc
      301 cttcctcgtc cggccgctgg gcgggctgtt ctgggggccg ctcggcgacc gcatcgggcg
      361 caagtcggtg ctcgcgatga cgatcctgct catggccggc gcgaccttcg cggtcgggct
      421 catcccctcc tatgacagca tcggcttctg ggcgccgacg ctgctcatcc tgctcagaat
      481 gcttcagggc ttttcgaccg gcggcgaata tggcggcgcg gcgaccttca tggccgaata
      541 tgcgcccgac gaacggcgcg gcttctatgg cagcttcctc gaattcggca cgctcgcggg
      601 cttctccttc ggcgcggcgc tgatgctcgg ctactcgctg cacctcggcg atcaggcgat
      661 gcacgaatgg ggctggcgca tccccttcct gatcgcgggg ccgatcgggc tcatcggcat
      721 gtatgtgcgc tcgaagatgg aggacacgcc gatctttcgc gaggaaatgg cgtcggccga
      781 acggcgcaac ccacccggca tcgcgatcat gctgcgcgac tattggcgtc cgctgctcgt
      841 cgtcggcggg ctcgtcgtcg cgctcaacgt cgtcaactat tcgctgctca gctacatgcc
      901 gacctatctc cagcggcgca tcggcctgtc gaacgaagag gcgctgctcg tgccgatcat
      961 cggcatgctc ttcatgatgg tttttctgcc cttcgcgggc gcgctgtccg accgcgtcgg
     1021 tcgccgcgcg atgtggcgct ggtcgctgat cgggctgctg ctcggcgtcg tcccgctcta
     1081 tatgctgatc gggacggggt tcaccggcgc gctgatcggt ttcatggcgc tcggcctgct
     1141 ttacgtgccg cagctcgcga cgatctcggc gaccttcccc gcgatgttcc cgaccgccgt
     1201 gcgcttcgcg gggttcgcga tcgcctataa tatatcgacc tcgatcttcg gcggcaccgc
     1261 cccgatgatc ggcagcggcc tgatcaccct gaccggcgat ccgctgatgc ccgcctatta
     1321 tatgatgctg gcgtgcctcg ccggcctcgc ggcgttgcgc ttcatgcccg aaaccgccgg
     1381 gcggtcgctg cgcgaagacc ctttcgcgcg aaaaacggcg ctcgcctccg acacctgata
     1441 ccatgccaca actcggttca tcgcaagacg cgcgatgcgg taagttttcg ttaacctcga
     1501 ttggccagac atacggacgg ggggcacgaa gcggccaatg ccgcgcgaac ggagagtcgc
     1561 agtgctcgaa gacatgcctg gccggcccgc cgcggtgatg ggtccattcg gcccgctgac
     1621 accgaaggat ttgccgtcgt cggacacgac ctattgggtc agccgccgca aggccgaagt
     1681 ccttgctgcc attaacggcg gaatgctcac catcgccgag gcttgcgcaa ggtatcggct
     1741 aagccgcgaa gaactggacg cctggcagcg atcgatcgat cgcgccggga tacccgggct
     1801 tcgcatcaca cgtctccagc aatatcgcgg acgccggatg gagtgagttt caatcggtgc
     1861 tgccaccccc cacctaaggc agcatcggcg gggcggtgca agcgatctgg tccccgcttg
     1921 cgccgccctt cttttcaggg gcgcggccgc cctgctaaag ctggcatttc cgcgttacgc
     1981 cgcgccggac ggtgtagcga tcggtcttcg gatcatagct ccggtacgcg cgcttgcacg
     2041 ccgcgacatg acgctgccag gacgccccct tccggttgcc cgcttgctgc gccagacgct
     2101 cctgcctgat cttctgctgg tgacggatac ggttgttgtc gcgctgcgaa ctcagatagg
     2161 aatcgagata ttgcgcgctt gccggcggcg ccgaaacgac tgtcgccgaa agcgatatgg
     2221 cgacagccgc gacgatcgcg atcgattttc ctgacatcgt cggcctccct ttttgagcgg
     2281 aacacaaacg cttgcccgga gccgatgttc ccgatcagat ccaactccgg tgcggaagtt
     2341 gcccggcagg atcagcgccg cgccatcgct ccacgatcat tccgggtccg cgcttttcta
     2401 ctcgcccaat ttttaatgcg tacgggttcg cggccggtcg tcatcaatat gtcactgcga
     2461 caatctctgg aggcgcggtc gaccgcggcc gattcggcgc acggcgacca aggagcatgg
     2521 ccgcgtcgaa atgcgcgaga atggcgatat catagcctgg attgcccggg aaattatccc
     2581 gcacgagggt gctatccgta aatggctggc acgccgctgg ggccacgtca tcgacgtcga
     2641 ggatgtgatc caggatgcct attgccgcct cgcgaacctc gcctccgtcg agcatatcga
     2701 taatcctggc ggttatttcc atcgaacagc gagcgcagtc gtcaccgacc atatgcggcg
     2761 cgcgggaata attaatttca cttcgatgac ccaaatcgaa tggtcgaacg tcattgatga
     2821 tgaccccttg cccgatcgca gcctggaaac gcggcaggaa tatgagcggg tcgccggact
     2881 gcttgcgatg ctgtccgaca cgggcaggaa ggtgatcgaa ttgcggcgta tcgagggtct
     2941 gtcgcgaaag gagactgccg agcgcctcgg cgtgagcgag aatgacgtga agaaccatct
     3001 cgttcgcgga ctgcagaaaa tcttgggagc gatggccgag caggatgcgc gatcgaaagg
     3061 cgatgcgccg gcggataccg ggaagaagga tgaagtgttt ggcaagcgcc ggccctatta
     3121 acgcaatcga cgaacaggcg gcggtgtggg cggtcgagac ggcgcatggc gagatgaacg
     3181 cccaaagccg ggcggatctc gaggcctggc tttcggccga tacgcggcat cgcggcgcct
     3241 atatccgcgc ctgcgcctgg ttgcgtgccg ccgagcatgt cgcgaccgcg gcccatcggg
     3301 cgcccgctgc gtcggtcccg atcgtcccct tgcgcacatc cgacaatgac aatgacgggg
     3361 acaatgaagg gatcgcgccc ggcgcattgc gcatgctggc ctcgcgctgg agcgggcgag
     3421 cggtcgccgg cggcgcggct gttgccatgt cgctggcatt gctcgtcgcg gtcggcgtgc
     3481 cggtgccggg gctcgtcacc ccgaccgaaa caagtgcgag cgccaagatc gtaaccctca
     3541 aggacgggtc ggtggcgacg ctcggccgcg atgcgcagat ccaggtgtcg ctgtccgagg
     3601 gtatccggaa gattaccttg ctgaggggcg aagcgacatt cgaggtggcg aaagacaaga
     3661 gccgtccctt tgtcgtgcgt tcaggcgacg tctatgcgca ggcgacgggg accgtctatt
     3721 cggtacgccg ggtcggcctc accggcggcg gcgtgcgggt cagcgaaggg agcgtgctcg
     3781 tatggccgcg cgacgagcgc gaccaagccg tcctccttca cgccggcggc acggtgacgc
     3841 tcgatccggg cccattggca acgacagccc aagcaccgcg accggcttcg cgccgcttgc
     3901 caccacccga actcgcacag atttcgctcg acaatgtacc ggtccgcgcc gccgtggcgc
     3961 gcttcaaccg agtcaactcg accaagatca tattagccga cccggcaatc ggggacatcg
     4021 agatcatcgg actctatcgc gccaacgatc ccgagcattt cgcggaagcc gttgccgcaa
     4081 tatcgggcgc ccatatcgaa cggtctggat caactatcgt aataaaaatg aagtaaaatt
     4141 gcctcccaat attaatatca atcctttctg atccgaatat gaatttcccc cgtcgatagg
     4201 ttcgagagca tcgaacaagg ggttttctga aatgcgttct tattacattc gttcggccgc
     4261 ctcggcgacg gcggtcgcgg ccgcgatgat ctttcccgcg gcggcccagg cacaggtccg
     4321 cagctttgac gtcccgaccc aatccgcatc aaatggaatt cgcgagttcg cgcgtcaggc
     4381 cggtatccag gtcacgctgg ccggccgcga tggtgacgac cgtacgacca atgcggtccg
     4441 cggcagcttt gaaatacgcg acgggctggc ccggcttctg gccgacaccg gcctgacagt
     4501 ccggtccttc gacggaaaag tcgccattct cgcagcgagt gcggccgatg tcgcggatgc
     4561 ggacgagacc atcacagtaa ccgggtcgcg catcgcccgc cccgaactgg aatcggcgat
     4621 gcccatcggc atcgtcagca tggaagcggg ccggaaggcc ggcaacacca ccgtttatga
     4681 aaccgtcctg cgcgacgtcg cggtggggcc cggcaacggg ccctataaca gcgcgcccga
     4741 gggacaatat gacggcggca tggcgaccat cgagctccgc aacatgggag tcagccggtc
     4801 gttgacgctg atcgacggcc gccgccgcgt ctcgggcgcc gcctcttcgt cggcggtcga
     4861 tatcaacatg atcccgtcgg gcatggtcga gcgcatcgag atcgtcacag gcggcgccgc
     4921 tgccatatat ggcgccgacg ccgtgacggg cgccgccaat atcatcaccc ggaaagacat
     4981 cgacgggctc gaagtatcgg caacgacggg gttcacccag gacggcggcg gaagcaaatc
     5041 gcagctgtca ttcgtcgccg gaaccagatt ttcagacgac cgcggatcga tcacgatcgg
     5101 cgggacctgg atgaagaaca acccgatcta tttccaccag cgctactcca gaaacacgaa
     5161 tatcagctac caggcgaatc cggaaaatac cggccccgac gacggcattc cggaccgcgt
     5221 catccggtac aatatgacga acatgtatct gcagcccaac gcgaacatct atgtgggcgg
     5281 caacacctat ttgctggcgc ccgacggctc ggcctatgtc ggccaatatg ccaatggctg
     5341 catctcgggc tgcggcactt cgcgcgacgg cggcgatggc gggtttccgc agagccaata
     5401 ttggagcgac ttcctgatcg cgccgatcga aaattacgcc ttcatcgggc ggttcgacta
     5461 tgcgctgacc gacggggtga attatgagtt ccgcgtcgac tatggccggt cgcgctacga
     5521 cgcctatcgc cgtccctatc gcgacgacga tcgcctgacc tggctcaacg gtgccggcgg
     5581 cgcgaccgcg cggctcgaca atccctatct gcccgattcc ttccgtcaaa tcatgctcgc
     5641 caacgatctg acatcgacag ccgtccgccg gagctatgaa aatttcggtc agatgaccga
     5701 ctatagcgat cgtcagagcc tgaccgtcag caccggtttc agcggtgaac tcgcggaccg
     5761 gttcaaatgg gaggttttcg gccaatatgg ccgcagcacc aacgacatcc atgccgacaa
     5821 cgtgccgatc gcgtcgcggc tgatcgccgc gcgcgacgtg atttcggacc ccgtttccgg
     5881 cgcaccggtc tgccgcgatg cggccgcgcg cgccaacggc tgcgttcctt tcaatatttt
     5941 cagcgaggcg ccgctgaccg ccgagcagcg cgggtggatg atggcaacgc gccgcaagca
     6001 ccgcgaacag acacaaaaga tcttcggcgc gagcatcacc ggcagcccct ttgcgctgcc
     6061 ggccggcgag gtacaagccg tgctcggcgc cgaacatcgc cgggaagcca tccacaatgt
     6121 cgacgacaat ggcgccgcgc cgcccgaaaa ggagttgtcg caccttggag cctgggcgcc
     6181 gtacgagccg gaacttcgcg cctcgaacca tgtgtcggaa gtctatggcg aactggtggt
     6241 ccccgttctg cgcgaccttc ccttcatcca caagctgacg gtcgagggcg ccgtccgtta
     6301 ttccgactat agcgattttg aatcgaccac gacgtggaaa gccggcgcca catggatgcc
     6361 cttctccgga ctgacgctcc gcgcggtccg ctcgcgaagc gttcgcgtgc ccaacttcgg
     6421 cgagcgctat tcggcgcgcg ccgtccagga aatcggcgtg cgcgatgcct gtctcgagac
     6481 gagctatcag gccaatccga cgcgcaaggc gaattgcgcc gcgctgatgc aacaactcgg
     6541 cattcctacg ccctatctgc ccagcgaaat cggtaccggc tatgtgatta ccggcggcag
     6601 catggacgtg accccggaaa cgtcgaacag cctcaccgcg gggctagtct ggcagccggg
     6661 tttcctttca gggttcgacc tgacggtcga ttactggaac atcaagatcg gcaacatgat
     6721 cgcggcgatc ggccggcagg acgtcctcaa cctgtgcatg gacctgccct cgatcgacaa
     6781 ccagttctgc gatcgcatca cgcgcgattc ccgcggctat gccgagggcg tcgacacaag
     6841 ctacatgaac gtgtccgaat ccgaggcgga ggggatcgac gtcggcgcga attatcggac
     6901 cgatgttggc cccggccgcc tcgcggtcgc ggtgcgcgcc agctacctca tcaagttcga
     6961 gacaaccacc ctgcccggcg tcgaaacgag ccggatcatc tacgacggcg gctatcaaaa
     7021 tccgcgcgtc cgcgccaaca tgttcgcaag ctacagcatc ggcgactggg aggtcgggct
     7081 caacacgcgg ctctggggca gcgcggtcaa ctacaccaat gtctcggacg aagcctatga
     7141 gcggaacaag ctgcccgcga aggtctacaa cgacctgaac gtgggctgga acctcaacga
     7201 aaatgcgacg ctgcgcgtcg gcatcaacaa tctgctcgat gtgcgcccgt cctatcgccc
     7261 gcggacctat tatcagggcg gcggcggcgt ttacgacgtt tatggccgct acttcttcag
     7321 cagcgtcaac ttcaaattct gaggagcgag gcgatgatca aatctctcca tttgcggcgc
     7381 tgcgccgtgc tcgccgccgc ggtcacctcg ctttcgtccc cggtcgcttt cgcagaggaa
     7441 gcaccggctg ccgcgagtat tgcacaaccg cggcccatcg cggtgatcgc acaccgcggc
     7501 ggggcgctgc ttcgcccgga aaataccatg ccggcttttc gtcacgcggc cgaaattcgc
     7561 gccgaatatc tggaattcga catggagatg acggccgatg accggatcgc cgtctaccat
     7621 gacgcgttga tcaatcccga cttttgcaaa caagacgacg gttcgcccgc gccggcccaa
     7681 ccggtacgca agctcggcct cgcagcgata caaaagctcg attgcggtac gggcgtaagg
     7741 ccgcaatatg ccggcccccg ccatgtcgcc gtgacgggcg cccgcgtgcc cgagctcggc
     7801 gaggtgctgc atgccttcaa aggcagcgaa gcgctgtttt ttgccgagac gaagataccc
     7861 aaggatgcgg atatcgaccc ggtcgaattc gcgcgcctcg tcgaagcggc ggtgcgcgag
     7921 aacggactcg aggacaggct catcctgcaa tcgttcgatt ttcgcacgat cgacgccttg
     7981 cacgagatca atccgcgcat ccgcacctgc ctgctcggcg tgccgcggct gacgcgcgac
     8041 tatcttgcta tgcttcgccg tcacaattcg aagtgtatcg tcctcggaat cggcgaggtc
     8101 gatcgtgccg ggacagcgcg gttgcaggag gccggcattc tggtcttctc gggtgtcgcc
     8161 gaccgcgaag aggagtggcg ccaatatgca gagctcggcg tcgatgcgct cttcaccaac
     8221 gatcccgaag gcgcgatcgc atatctggag cgatccggat tgcgcaacta agcttccccg
     8281 gcgacgccgc tgtcacgcgg cggcgccgct cctttccctc cgccacgaaa ggcaccgcaa
     8341 acatggcctt cgcaaagacc ctcgatcgcc gcaaccttat caaagctgcg gggagccttg
     8401 ccgtgctggc gggcttctcg cgcgcgccac tgttcgcccg gccgctcggc gccaacccgt
     8461 tcaccctcgg agtcgcgtcg ggcgatccgt ggcccgacgg cttcgtgatc tggacgcgcc
     8521 ttgcgccgcg gccgttcgac gaacatgccg gcatgcccgc ggcacgcgtg ccggtgcgct
     8581 gggaggtcgc cgaagacgcg gcctgtaccc gcatcgtgca gagcggcgaa acgctcgcga
     8641 tgcccgaact cggtcactcg gttcacgtcg aggtcgccgg actccggccg catcgcccct
     8701 attggtaccg tttcgcggtc gccggatcgg atgtcagtcc gatcggcacc gcgcgcaccg
     8761 ccccggcaag cgatgcgatc gtcgaccggg tccggatcgg cgtggcagga tgccagcatt
     8821 acgaggtggg ccattatgaa gcctgggccc atctcgcgaa cgaacccgat ctcgatctca
     8881 tcttccacta tggcgactat gtttacgagg gcgggacaac gccgctcggg ccgcaggcca
     8941 gcggcgtgcc gatcgtccgc cagcatatcg gcggcgagat ttacagcatc gaggattatc
     9001 ggcgccgtta cgcccaatat aagggcgacc cggctttgca ggcggcgcac gcggcctgcg
     9061 ccttcgcctg ctcgttcgac gatcatgaga tcgacaataa ttgggcgggc gacttcgatc
     9121 aggatggcac cagtccggag atattcgcct tgcggcgcta cgccgcgctg caagcatggt
     9181 acgagaatat gcccgtccgc aaggccctgt ttcctgctca aggcgggctc accgcctatc
     9241 gccggctcga ttacggccga ctgctgcgca cgcatgtgct cgatacgcgc accaaccgct
     9301 ccgaccaggt ttgcaacgac ggaaagatcc gcccctgccc gcccgatcgt ctccagtcgc
     9361 ccgagatcct cggacgcgtc caggaagcat ggctcgacga gggactcggc aacgacgccg
     9421 tctggaatct gctcgcgcag cagatcatcg tcatgccggt cgatttccgc gccgacgacg
     9481 ccgccgaagc gcgcttcgcg accgacctct gggatggcta tcgcccggcc cgcgagcgcc
     9541 tgatcgagtc catccgcaag catgaactga ccaatgtcgt gatcgcaacg ggcgatcatc
     9601 acaaacatct ggtgggctcg gtgcccgaag atgacgatcg acccgacggt ccgaaagtcg
     9661 ctgtcgaatt ccaggcggcc tcgatcagct cgaacggcaa cggtaaaggc gaagccgggc
     9721 ttgagcagat gatgcgaaac aacccgcact ttgacctcta caccgaccgc cgtggctatc
     9781 agctgttcga gatcacgccg aaggaatggc gaaccgaagt caaagtcatg gatcaggtgg
     9841 aaaggccggg cggaacgatc ggcacgcttg cccgatatgc cgtctccccc gacgctccaa
     9901 tccttcaccg agaataatca acaacgatca atttagcctc tgccgacgga ttgcagccgg
     9961 tcgactttcg ggcgaggcgc tcgactttgg acgtgcatcc ccttgaaatc ttgatacatg
    10021 cgcgctcgcg atcgcttggg gcgtagagcg attgcgacct ctcgggggac ccaagagcaa
    10081 caatctaagg attatcgaac atgaagaagt ttttggcggt cgccgcactg ggcgcggcgc
    10141 tggcactggg cggctgcggc aagagcgacg ataaggcagc tgcggccggg acggtgaagc
    10201 gcgaagccgg caactggaag accgatatca aactggtcaa gttcgagatg ccgggcgtcc
    10261 ccgacaatgt gaaagaccag atgtcgaagc agctagcggc cgccagcggt acggaacaat
    10321 gcctcacgca ggaacaggcc gacaaggaag atgtcgccgg tgcgctgtcg aagggctatg
    10381 gcgaggcctg tacctggagc aagaaccaga tcggcgatgg taagatcgac gtcgcaggca
    10441 catgcacaca aggcgcacag aaaatcgaac tcgcccttgc cgggaccctc ggcgcaaaga
    10501 aagccgatgt tctcgtgact tcgaagggca agtcgccgat gggcggcggc gcgatggaaa
    10561 tgcagatgca ggtcaccagc accaatatcg gtccctgcaa aggctgagct tcgcgaaaaa
    10621 acgagtgaat gacggggcgc gatctgggat cgcgcccctt tttgcgcgca ccggatcgca
    10681 acccgacgtc ccgcccattt cgacgcgaaa tggcatccca aaccgaaaca cgattatgaa
    10741 atcgataggc tgatccagag aaggcgcgct ttgccaacct ctcgtcaatc cggccaaacc
    10801 atcgaaataa tggcaggggc agcaggactc gaacccgcga ccctcggttt tggagaccga
    10861 tgctctacca actgagctat acccctaggc cggagtgcgc cactagccgg attggagggg
    10921 cggggcaagg gggatgacgg cttgcgcgcg cgagccgggc tgatatgcgc aatttccaat
    10981 gagcgacgcg tcgtcccccg cactccccat ctatgggcct ccgcagcatt atctgggcgg
    11041 tatcgcgttg cgactgcttg cgatggtcag cctgtcgctg atgttcgtgc tcgtcaaaaa
    11101 gatcgacgcg gcgggcatcc atgtcgtcga aagcctgttc tggcggcagg cgctcgtcct
    11161 gcccttcctg ctcgcctggg tgatggcgac aagcggtttt tcgtcgctga agacgcggcg
    11221 gatcggcgcg catgcacggc ggatgctgat gggcctgacc ggcatggtgt gcaatttcgg
    11281 cgcgatgatc ctgctgccga tggccgaggc cacgacgatc agcctctcgg tgccgatctt
    11341 cgccgtcatc ttcgccgcca tcctgctcgg cgaggcgacg ggatggcagc gatggagcgc
    11401 ggtgatcgtc ggcttcgtcg gcgtgctcgt cgtgctcgat ccgctggcga gcttcgcggg
    11461 cggtttcggc ggaacgcacg gcgccggcac attggtcgcg ctgaccggcg cgatcatgac
    11521 cgcgctgatc accattgcgg tgcgcgatct tggccgcacc gaaaatgctg cgacgatcgt
    11581 cttctggttc agcctcctgt cgatgatccc gctcggaata gccctgccct tcgtcatcac
    11641 cccgcacgac ggccacgaat ggctgctgct gatcgggctc ggctttctcg gcgcggtcgt
    11701 ccagatgtcg ctgaccggcg cgctgcgcct cgcaccggtg tcggtggtga tcccgatgga
    11761 ttattcgagc ttgctgtggg cgatcgccgc gggctggttc tttttcggta ccctgcccgc
    11821 cgacacgacg tgggtaggcg cgccgctgat catcgcgtcg ggcctgttca tcgcctggcg
    11881 cgagcatcgc cgccatatcg agcgcccgaa ggaggttgcc gcatgacgct gcccattctc
    11941 taccactgcc ccgatgcccg ctcgctgcgc tgcctgtggg cggtcgaaga ggcggggatc
    12001 gatgtcgacc tcaagctgct caaattcccg ccgcgcgcgt tcgaacccga ttatcgcgcg
    12061 gtcaatccgc ttatgacgat tcccggctgg gtcgaggacg ggcggctgat gaccgaatcg
    12121 gcggcgatct gcgagcggat cgccgagggc acgccgctcg aagtccgccg cgacgaagcc
    12181 gattattggg cgtggcgcaa ctggctgcac cgcagcgacg cgacgctgac cttcccgctc
    12241 gcgatcatga tccgctatac gcgcgtcgag cccgaggagc ggcggctggc gcaggcggtc
    12301 gaggattaca aagccttctt cggcggccgg gcgaagagca tcaaggcgga gctcgccgac
    12361 gggcgcgagt ggctcgtcgc ggggcggttc accatcgccg acatagccat cggctatgcg
    12421 gccttccttg cgacgacgct cggcgccggc gatgtgttgg gggatgcgac gagggcatgg
    12481 ctcgatcgct gcatggcgcg cgaagggttc cgacgcgcgc gcgaacgaca gaaagccgcc
    12541 gcgtgacgaa aaaggactcc accataaggt gaagccctcc gtgccccccg tgcatggcag
    12601 cgccaacgag gaaaacgcat cggcctttcg gggtgcgggt cgcgtgaaag gccggatatg
    12661 gggtgagttt atggcgcggc ggggaggctg gctttcacat tctcactggg gacgcgccac
    12721 cgctttttta gacacttgac gcggcggctt cgatagctcg gggctcaaat agggaggctg
    12781 cgcgtcgcga tcacgcccca gcaagcgaag catcgtaata ttatagtccc acgcgtagcg
    12841 cgtttcgcca tcgcaaaggg ggccgatact tccaacctga ctcagatcaa ggtcgtccaa
    12901 ggtcaggccg gccaccggat aatcattgcg cacttctgcc gttgcatcgt cgtgcagacc
    12961 gggagtccat cttttgtaaa caggatcatg ggattccaca gcttgcaaca accccttgcg
    13021 accgcttcgg aactcgaaat ccgcatcggc aattccgctt cgatattgcc aatacatcag
    13081 gcagcgaagc cgcgcttgcg cgatgcggtg agacctttcc tgcgctgcct gtacaccgct
    13141 caaacgccac acggtcgcgc tcgcgcctaa cagcgccgag agtatcgcca ccagcgcaac
    13201 gcgaagcgcg aggcgccggc cgttcaattc accatccgct catagccttc ccaatatggc
    13261 gcacgcaggt ccttgcgcag gattttgccg ctggcgttgc gcggcaacgc ctcgatcacg
    13321 tcgatgctgc gcgggcattt gaacggcgcg atgcgttcgc gcgcccacgc gatgatgtcg
    13381 gcttcgtccg ccgtggtgcc cggcttggcg acgacgaccg ccttcaccgt ctcgccccat
    13441 ttcgtgtcgg ggatgccgat caccgcgact tcctgcaccg cggggtgacc gaagatcgcg
    13501 ctttctacct cggcggggta gacattctcg ccgccggtga tgatcatgtc cttcatccgg
    13561 tcgtggatga aaagatagcc gtcggcgtcg aggtaacccg cgtcgccggt gcggatccag
    13621 ccgtcgtcgg tcatcgtctt cgcggtcgca tcgggcaggt tccagtatcc gatcatattg
    13681 ttcgacgagc gggtgacgac ctcgcccacc tcgcccgtcg gaaccggttg gccgtcgggt
    13741 ccgaggatga cgatctcgac cccggggagc gccttgcccg ccgaacgcat gcgcgcgttg
    13801 cccgccggat catgatcctc gggcggcagc atcgaaatgg tgcccgtcgt ctcggtcatg
    13861 ccgtacgcct ggatgaattg cgcgccgaac atcttgatgc actggcgcag cagttcgagc
    13921 gggatcggtg cggcgccgta gaggatatat ttgaggcggc tatagtcgac gctggcgcag
    13981 cgcgggtgca tcagcagcat ctggagcgcg gcggggacga tgaagaagcg cgtgacgcca
    14041 tgctgctcga ccgcgtcgaa cacgccatcg gggttgaatt cggcaaggat gacgcccggc
    14101 agtcccgatg cgagcgccat gatgcccagc cccgtgccgc cgatatgcgc gcacggcatc
    14161 gcgaccagca ccgcctcgtc atcctcccat ttggtgtagg gcaggtcgag ggtgctcgaa
    14221 tgtttgcgca gcgcgaagag gttgtggttc gacagcaccg cgcccttggg attgccggtc
    14281 gtgcccgagg tatagagctg gagcaccgca tcgttggcac ccgacggctc gaagggcgcg
    14341 cgcgccgcgc cctcgatcat cgcccatgcg tcggcggcgc tcatgatcgc cggatcgttc
    14401 gcgagcttgc ccgcgagctg gtcggccaat ccctcgaacc ccggcccggc gaagacgatc
    14461 ttcgcctgcg tgtcattgac gatgaacgcc cattcggccg gcgacaggcg ccagccgatc
    14521 ggcgccatca cgatgcccgc gcgcgcggcg ccataaaaaa gggtgaaata gaggtcgctg
    14581 ttcttgccga tccaggcgat gcggtcgccc tttttcagcc ccgccgcgat cagcgccgag
    14641 gcggcgcgtg cggtgcggtc gtcgagttgg gcataggagt aaacgcggtc ctcttcgcgc
    14701 atcgcgaccc gcccgggacg ctcggcggcc caatgggtca ggaattcgtc gaacgtaaac
    14761 agctccgaaa cgtcgcggcc catcgcctct ctctcctcga atcgcgtcgt ttgcgcgcaa
    14821 ttgatgagaa agctaacggt tctaccggcg aggtaaagcg ccgatcggcc gaccctgtca
    14881 ggtccgtcag gtgcgacgga agagcagcat cagattattc gccggcatcg cccggcgctc
    14941 ggcgaaggca agtccggcgt cgcgagcggc cgacttgacc ctatcggtgt cgcgcagccc
    15001 ccacgccgga tcgcgcgcgc gcagattggc gtcgaagtcg aggttgctcg ccgcggtcgg
    15061 cacatcgggt tcgatataag gtccgtagag gatcagcggc gcgcccggcg cgagcaatcg
    15121 cgcggcgccc gcgaacaggc cgagcgtcgc ttgccacggg ctgatgtgag ccatgttgat
    15181 gcagaggatc gcatcggccc ggtcgaccgg ccagcccgat gccgaggcgt cgagcgccag
    15241 cggcg
//
LOCUS       DATMSU010000145         4578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_40670, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000145 DATMSU010000000
VERSION     DATMSU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40670"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1795
                     /gene="lon"
                     /locus_tag="VN034_03875"
     CDS             <1..1795
                     /gene="lon"
                     /locus_tag="VN034_03875"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWV59768.1"
                     /translation="QVEKKIRGRVKRQMEKSQREYYLNEQLKAIQRELGNDNGEGGDD
                     LAELQLKIDSLKMSKEAKAKAQAELKKLRAMAPMSAEATVVRNYLDTLIGLPWGKKSK
                     LKKDIAKAQAVLDDDHYALEKVKDRIVEYLAVQARTNKLKGPILCLVGPPGVGKTSLG
                     RSIAKATGREFVRQSLGGVRDEAEIRGHRRTYIGSLPGKIVTNLKKAGTMNPLFLLDE
                     IDKLGQDFRGDPASALLEVLDPEQNGKFQDHYLEVDVDLSDVMFVTTANSLNLPQPLL
                     DRMEIIRLEGYTEDEKVEIAKLHLIAKQVEAHGLKDGEFELTEDGLRDLIRYYTREAG
                     VRTLEREIARLARKALRRILEGKATSVTVTPENLSEFAGVRKFRHGMGDLEDQVGAVT
                     GLAWTEVGGELLTIEAVTASGKGQVRTTGKLGEVMTESVQAALSFVKARAPAYGIKPS
                     LFARKDIHIHLPEGAVPKDGPSAGVGMVTAMISTLTGIAVRRDVAMTGEVTLRGRVLA
                     IGGLKEKLLAALRGGIKTVLIPEENEKDLVEIPANITSGLKIIPVSHVDQVLAEALVS
                     PVVPIEWTEADELAASPPVPPGSDPESAIRH"
     gene            1964..2236
                     /locus_tag="VN034_03880"
     CDS             1964..2236
                     /locus_tag="VN034_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWV59769.1"
                     /translation="MNKQDLIAAVADSSGLTKGDASKAVEAVFDAITGSLKKGGEVRL
                     VGFGTFAVSKRKASTGRNPRTGETMTIAASNQPKFKAGKALKDAVN"
     gene            2410..2484
                     /locus_tag="VN034_03885"
     tRNA            2410..2484
                     /locus_tag="VN034_03885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2442..2444,aa:Val,seq:cac)
     gene            2572..2880
                     /locus_tag="VN034_03890"
     CDS             2572..2880
                     /locus_tag="VN034_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59770.1"
                     /translation="MTRTLTLSLISALLLSAGAPAVARGDRDQDNLRQAAAQGQVVPL
                     GKLVADIRSRPPYNDMTYLGGPQFDAGRMLYKLKFMDGSQVVVVYVDARTGRIVGRKP
                     "
     gene            2998..3660
                     /locus_tag="VN034_03895"
     CDS             2998..3660
                     /locus_tag="VN034_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV59771.1"
                     /translation="MRILIVEDEPTLGPQLKATLEGAGYAVDLATDGEDGHFLGSTET
                     YDAAILDLGLPEIDGLTVLDRWRREKRNFPVLVLTARDSWSDKVAGLDAGADDYLAKP
                     FQTEELIARLRALIRRASGNASSELIAGDVRLDTRSGRVTLAGEPVKLTAQEYKLLSY
                     LLHHKGKVVSRTELIEHIYDQDFDRDSNTIEVFVTRIRKKLGADIITTIRGLGYQLDD
                     PQ"
     gene            3766..>4578
                     /locus_tag="VN034_03900"
     CDS             3766..>4578
                     /locus_tag="VN034_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWV59772.1"
                     /translation="MIAIAALWISVLLIGGGFALDQVLKGAITRNFDSGLEYVLIAMI
                     RSSEIGPDGEVRLVEPLGDQRFLEPYSGLYWQISGGNQEPYRSRSLWERTLKAPTPHV
                     DNQIHTYDSIQFPDEELRVLERNVILPGSDINWRYQIAQSREALDAQVRAVRQTLIPS
                     LVLLGLGLIILAALQTFYGLWPLRHIRRAIAAMRGGQNRRVTAPLPLEVQPMVDELNA
                     LLAHNEKQAEEARLHAGNLAHALKTPLTVLVNSATSSDSELAETVRREAATMQ"
BASE COUNT          902 a         1465 c         1458 g          753 t
ORIGIN      
        1 gcaggtcgaa aagaagatcc gcggccgcgt gaagcgccag atggaaaaga gccagcgcga
       61 atattatctc aacgaacagt tgaaggcgat ccagcgcgag ctcggcaacg acaatggcga
      121 gggcggcgac gacctcgccg agctgcagct caagatcgac agcctcaaaa tgtcgaagga
      181 agccaaggcg aaggcgcagg cggagctcaa gaagctgcgc gcgatggcgc cgatgtcggc
      241 cgaagcgacc gttgtccgca actatctcga cacgctgatc ggtctgccgt ggggcaagaa
      301 gtcgaagctc aagaaggata tcgcgaaggc gcaggccgtc ctcgacgacg accattatgc
      361 gctcgaaaag gtcaaggacc ggatcgtcga atatctcgcg gtgcaggcgc gcaccaacaa
      421 gctcaagggt ccgatcctct gcctcgtcgg ccctccgggc gtcggcaaga cctcgctcgg
      481 ccgaagcatc gcgaaggcga cgggccgcga attcgtgcgc cagtcgctcg gcggcgtgcg
      541 cgacgaggcc gaaatccgcg gccaccgccg cacctacatc ggctcgctgc cgggcaagat
      601 cgtgaccaac ctcaaaaaag ccggcacgat gaacccgctg ttcctgctcg acgagatcga
      661 caagctcggc caggatttcc gcggcgatcc ggcatcggcg ctgctcgaag tgctcgaccc
      721 cgaacagaat ggcaagttcc aggatcatta tctcgaggtc gacgtcgacc tgtcggacgt
      781 gatgttcgtg accaccgcca actcgctgaa cctgccgcag cccttgctcg accgcatgga
      841 gatcatccgg ctcgaaggct ataccgagga cgagaaggtc gagatcgcca agctgcacct
      901 gatcgcgaag caggtcgagg cgcatggact gaaggatggc gaattcgagc tgaccgaaga
      961 cgggctgcgc gacctcatcc gctattatac ccgcgaagcc ggcgtccgca cgctcgaacg
     1021 cgagatcgcg cggcttgcac gcaaggcgct gcgccgaatt ctcgaaggca aggcgacaag
     1081 cgtcaccgtc acgccggaga atctttccga attcgccggc gtgcgcaaat tccgccacgg
     1141 gatgggcgac ctcgaagatc aagtcggcgc cgtcacgggc ctcgcatgga ccgaagtcgg
     1201 cggcgaattg ctgacgatcg aggcggtcac cgcgtcgggc aaagggcagg tccgcaccac
     1261 cggcaagctc ggcgaagtga tgaccgaatc ggtgcaggcg gcgctgtcgt tcgtgaaggc
     1321 gcgggcgccc gcctatggca tcaagcccag cctgttcgcg cgcaaggaca tccatatcca
     1381 cttgcccgag ggcgcggtgc cgaaggacgg tccgtcggca ggagtcggca tggtgaccgc
     1441 gatgatctcg acgctgaccg ggatcgcggt gcgccgcgat gtcgcgatga cgggcgaagt
     1501 cacgttgcgc gggcgcgtgc tcgcgatcgg cggcctcaag gaaaagctgc ttgcggcgct
     1561 ccgtggcggc atcaagacgg tgttgatccc cgaggaaaat gaaaaggacc tcgttgaaat
     1621 cccggcgaat atcacgagcg gactcaagat catcccggtc agtcacgtcg atcaggtgct
     1681 ggccgaagcg ctggtatcgc cggtcgtgcc gatcgaatgg accgaggccg acgagcttgc
     1741 ggcgtcgccg ccggtgccgc ccggaagcga ccccgaatcg gcgattcggc actgaggcac
     1801 cgccgcttga tggcgtttca ccccaaattc gtcgcaaaat cgacgttttg gaacgttttt
     1861 cgcccttttt tgctttgaca agacaggggc aaacatcgtt agtggcgcgc catggcttcg
     1921 ggccatgaga atcattcaac cataatgcag gggttcctta ggcatgaaca aacaagacct
     1981 gatcgccgcc gtcgctgact cgagcggcct gaccaagggt gatgccagca aggccgtgga
     2041 agctgtcttc gacgcgatca ccggctcgct gaagaagggc ggcgaagtcc gtctcgtcgg
     2101 cttcggcacc ttcgcggtca gcaagcgcaa ggcatcgacc ggccgcaacc cgcgcaccgg
     2161 cgaaacgatg accatcgcgg cgtcgaacca gccgaagttc aaggccggca aggccctcaa
     2221 ggacgccgtc aactaagttg gcccgcgcct tttggtaaaa acatctcggg ccgtagcgca
     2281 agctgcggcc cgatttgtat ttgcgggcga tgtttggcgt gaagattgag caaatgcccg
     2341 ttgcattcac cgcgccagcg gctatggacg tccggctttc ggacgccggg cttcggtccg
     2401 caccgggacg ggcgcgtagc tcagcggtag agcacaccct tcacacgggt ggggtcacag
     2461 gttcaatccc tgtcgcgccc accatggtcc gccgggccat ggtggaaccg aaaccgcggc
     2521 tgacccgccc attcatcgcc tattcaatgc tttcgcgtta gagaaatggc catgacccgc
     2581 accttgaccc tttcgctgat ttccgccctg ctgctgtcgg caggcgcccc cgcggtcgcg
     2641 cgcggcgatc gcgaccagga caatctgcgc caggcggcgg cgcaggggca ggtggtcccg
     2701 ctgggcaagc tggtcgccga catccgttcg cgcccgccct acaacgacat gacctatctt
     2761 ggcggaccgc agttcgacgc cgggcggatg ctctacaaac tgaagttcat ggacggcagc
     2821 caggtggtcg tcgtctatgt cgatgcccgc accggccgga tcgtcggccg taaaccctga
     2881 caattcccaa agaccgcccg tttcgtcgca acgagatttt gcgatggacg ttcggacttg
     2941 atacacagtc gaaacatgcg gccgcccggt tcggtttcgc aaaggaaagg cgcctgaatg
     3001 cggattttga ttgtcgagga cgaacccacg ctgggcccgc agctcaaggc gacgctcgag
     3061 ggggcgggct atgccgtcga cctcgcgacc gatggcgagg acgggcattt tctcggctcc
     3121 accgaaacct atgacgcggc gatcctcgat ctcggcctgc ccgaaatcga cgggctgacc
     3181 gtgctcgacc gctggcgccg cgaaaagcgc aatttcccgg tgctcgtgct gaccgcgcgc
     3241 gacagctggt cggacaaggt cgccgggctc gatgccggcg ccgacgatta tctggcaaag
     3301 cccttccaga ccgaggaact gatcgcccgc ctgcgcgcgc tgatccgccg cgcgtcgggc
     3361 aatgcctcga gcgagctgat cgcgggcgac gttcgcctcg acacgcgctc gggccgcgtc
     3421 acactcgcag gcgagccggt gaagctcacc gcgcaggaat ataagttgct gtcctatctg
     3481 ctccaccaca agggcaaggt cgtgtcgcgc accgaactga tcgagcatat ctacgatcag
     3541 gatttcgacc gcgattcgaa cacgatcgag gtgttcgtca cgcgcatccg caaaaaattg
     3601 ggtgccgaca tcatcacgac gatccgcggg ctcggctatc aactcgacga tccgcaataa
     3661 gcgatgtcga cggccgacgt cgccccggcg gtcgaaaccg acccggacgc ggcaatcaag
     3721 cccatagtcg cgtcgggccg catcaccggc tcgctggcgc ggcgcatgat cgcgatcgcg
     3781 gcgctctgga tttcggtgct gctgatcggc ggcggtttcg cgctcgatca ggtgctgaag
     3841 ggcgcgatca cgcgcaattt cgattcgggc ctcgaatatg tgctgatcgc gatgatccgc
     3901 tcgtccgaaa tcggtcccga tggcgaggtg cggctggtcg agccgctcgg cgaccagcgt
     3961 ttcctcgaac cgtacagcgg cctctattgg cagatcagcg gcggcaatca ggagccctac
     4021 cgctcgcgct cgctgtggga acgcacgttg aaggcgccga caccccatgt cgacaaccag
     4081 atccacacct atgacagcat ccagtttccc gacgaggaat tgcgcgtgct cgaacgcaat
     4141 gtcatcctgc cgggcagcga catcaattgg cggtatcaga tcgcgcaatc gcgcgaggcg
     4201 ctcgatgcgc aggtccgcgc ggtgcggcag acgctgatcc ccagcctggt gctgctcggg
     4261 ctcggtctta tcatcctggc ggccctccag accttctatg gcctgtggcc gctgcgccat
     4321 atccggcgcg cgatcgcggc gatgcgcggc gggcagaacc gccgcgtcac tgcgccgctg
     4381 ccgctcgagg tgcagccgat ggtcgacgag ctcaacgcgc tccttgcgca taacgagaag
     4441 caggccgagg aagcgcggct ccatgccggg aacctcgccc atgcgctcaa aactccgctc
     4501 accgtgctgg tcaacagcgc gaccagctcg gacagcgagc ttgccgaaac ggtgcggcgc
     4561 gaggcggcga cgatgcag
//
LOCUS       DATMSU010000146        13967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_88069, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000146 DATMSU010000000
VERSION     DATMSU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 13967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13967
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88069"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2098
                     /gene="metE"
                     /locus_tag="VN034_03905"
     CDS             <1..2098
                     /gene="metE"
                     /locus_tag="VN034_03905"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HWV59773.1"
                     /translation="STVTKINALVVFPFESAALTKPVAQVKAKGAYVTVVDRGLTDTS
                     AQEMTKWFDTNYHFMVPELTAGQTFKIASTKIFDEFEEAKALGYQTRPVLLGPVSYLM
                     LAKGKAVEPLALLPQLLDAYAEILARLAKAGAEWVQIDEPCLVLDLSAEQRTAFERAY
                     ARLATRGPKLMLTTYFGGLGDNLGLVADLPVHGLHLDLVRAPGQLEPALTKLQPHVLL
                     SLGVVDGRNIWRANLPDLYWRLKELAGKRDLILAPSCSLLHVPVDLVLETSLDPEIAQ
                     WLAFAAQKIEELAALAKALNEGEDASFAAFSASRIAAVARQTSPKIHNPAVAARLAAL
                     DESATNRASPFADRRDAQQKRLALPAFPTTTIGSFPQTPEVRKARAAHIKGEIDDAAY
                     DRYLRVETRAAVKWQEEIGLDVLVHGEFERNDMVQYFGEQLAGFAFTRAGWVQSYGSR
                     CVRPPILYGDVSRPAPMTVGWWRYAQDQTDRPMKGMLTGPVTILNWSFVRDDQPRAQS
                     CRQIALAIRDEVEDLEAAGAAIIQIDEAALREGLPLRRSEWQHYLDWAVESFRIAAAG
                     VRDDTQIHTHMCYSEFNDIIRAIGDMDADVISIETARSRMELLDAFASYAYPNEVGPG
                     VYDIHSPRIPASQEMTDLLTLAAKHVPPAQIWVNPDCGLKTRKWDEVRPALIAMVAAA
                     KSMRARALVKEAYLAK"
     gene            2103..2495
                     /locus_tag="VN034_03910"
     CDS             2103..2495
                     /locus_tag="VN034_03910"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWV59774.1"
                     /translation="MPPDRMPEGQPAVRVTAMPANANVYGDIFGGWLMAQMDLAASSV
                     ASLHARGRAVTISVEGMTFHRPVFVGDEVSVFGRLVKVGRTSMHVEVETWARDRHADE
                     AHKVTQAVFIFVAVGPDRRPRAVRPLTD"
     gene            2676..5348
                     /gene="acnA"
                     /locus_tag="VN034_03915"
     CDS             2676..5348
                     /gene="acnA"
                     /locus_tag="VN034_03915"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWV59775.1"
                     /translation="MTTTGHDTLGTRSTLDVGGKSYAYYSLDKAAAKLGDVSRLPFSM
                     KVLLENLLRFEDGGFTVSKDDVQALVDWQKDPHSNREIQYRPARVLLQDFTGVPCVVD
                     LAAMRDAIATLGGDTTKINPLVPVHLVIDHSVMVDEFGHPKAFEQNVEIEYYRNGERY
                     DFLKWGSKSLDNFKAVPPGTGICHQVNLEHIAQAVWSSADGSGETVAYPDTCVGTDSH
                     TTMINGLGVLGWGVGGIEAEAAMLGQPVSMLIPEVVGFKFTGALKEGVTATDLVLTAT
                     QMLRERGVVGRFVEYFGPGLASLSLADRATLANMAPEYGATCGFFGVDDKTLDYMRLT
                     GRPEENIALVEAYAKAQGLWIVEGAADPIFTDTLELDLSTVVPSLAGPKRPQDRVSLP
                     DVDDVFNADMAKVYNKAQARVPVEGKDFDIGDGDVTIAAITSCTNTSNPGVLVAAGLV
                     AKKADALGLKPKPWVKTSLAPGSQVVTDYLEKAGLQKHLDNIGFNLVGYGCTTCIGNS
                     GPLAEPISKAINENGLVAAAVISGNRNFEGRVSPDVRANFLASPPLVVAYALKGTVIE
                     DFTTTPIGQDQSGNDVFLKDIWPTNQEVAETVAGAVDRDMFEARYAHVYKGDEHWQKI
                     EVEGSDTYQWRAGSTYVANPPYFEGMTMTPAPVSDIVSAKPLAILGDSITTDHISPAG
                     SIKADSPAGKWLMEHQVSKADFNSYGARRGHHDVMMRGTFANIRIKNEMVPGIEGGMS
                     RYGAEVMPIYDAAMRHKADGTPLVVIAGKEYGTGSSRDWAAKGTNLLGVRAVIVESFE
                     RIHRSNLVGMGVLPLQFLEGQSRETLGLTGDDQFTITGVADLKPRQTVTVNVTRADGS
                     TFSFDTLCRIDTANEVEYYMNGGILHYVLRKLAA"
     gene            5422..5697
                     /locus_tag="VN034_03920"
     CDS             5422..5697
                     /locus_tag="VN034_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59776.1"
                     /translation="MDAETIVVEVIGWTAAAIILAAYILLSLGKLEGRSYFYQWMNVI
                     GAGGFIVNSGYNGALPSAVLNVIWAGMGLFTLWSVWRARQAARAAIP"
     gene            complement(5694..7151)
                     /locus_tag="VN034_03925"
     CDS             complement(5694..7151)
                     /locus_tag="VN034_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV59777.1"
                     /translation="MKKLFLTFAPALLAFAPSALAAPKQGDVIIRHVSIVDVEAAKTI
                     AGQAVVLQGDDIVAVGADGAIAKDWHAARTIEGNGRYLIPGLWDMHVHFGGGPDLIEE
                     NKALLPLYIANGITTIRDCSGDLPEQVLAWRGEIARGSLFGPRLLTSGAKIEGIAPVW
                     KGTIEVGSEADVDAALDRLKNRDKVDFVKITDSTLKPELFLYALRQAKTLGLKTSGHI
                     PMALTVDQAVDAGISSIEHLDYAYNAGAKDEAAIAADFAAKRIDRAEANRRLDAGFDR
                     DTAMAAYRRFAAKGVFVTPTLNGSRIIAYLDRDDHSKDEGLAYIGPKLRKTYDWRIER
                     AAKADAAAIAARHKQIEDVATILPMLAEAGVPIIAGTDAGFLNSFNYPGFGLHDEIEL
                     FTAKGLTPAQALASATRAGPAWFGQLDRYGAVKPGMAADLVLLTKNPLEDIAATRAID
                     TVVMRGMVQDRAALDKLLADTRAKVAAWNAEAAAR"
     gene            complement(7200..7697)
                     /locus_tag="VN034_03930"
     CDS             complement(7200..7697)
                     /locus_tag="VN034_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV59778.1"
                     /translation="MPTKLDGSCRCGAVRFSVMSHTQVPYQLCYCSICRKQQGGGGYA
                     INLGADATSLTIEGEEHLGLYRARIEDDEHPSCKLSSGERRFCRGCGSALWLYDPNWP
                     DLVHPFASAIDSELPAAPSRVHLMLKYKASWVEPAIGPGDAAFDLYPEKSIEEWHKDE
                     GVWVA"
     gene            complement(7761..7836)
                     /locus_tag="VN034_03935"
     tRNA            complement(7761..7836)
                     /locus_tag="VN034_03935"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7801..7803),aa:Val,seq:tac)
     gene            complement(7913..8884)
                     /locus_tag="VN034_03940"
     CDS             complement(7913..8884)
                     /locus_tag="VN034_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HWV59779.1"
                     /translation="MPIMAARLYHDGKLVRDLGPDEAIPEDCDTGDFFWLGLYEPTPG
                     ELAGIAKRFGLHPLAVEDALKANQLPKVETYGDQLFIITRTANLDGDTIQPGETAFFI
                     GPHFFVSVRHGSARTHTDVRARLESLPTKLAHGPDYVLYAVLDFIVDGYFPVIDAIEE
                     RMLAVEDSVMDTPLDAKEIRHLYKQRHEIIRFQRLVGMMKEVAGRLTTDDDLPNIDPA
                     VRPFYRDVWDHVQRAEYRLNGLRDIAASVIETNGMLEQQRQGVITRQLAAWAAILAVP
                     TAIAGIYGMNFDHMPELGWAFGYPLIVGAMGTICLTLYWRFKHIGWL"
     gene            complement(8960..9472)
                     /locus_tag="VN034_03945"
     CDS             complement(8960..9472)
                     /locus_tag="VN034_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59780.1"
                     /translation="MKNEAEVVVNDRMQRGYRYTRAAPMGRAFDPDFEPELTPAEMLK
                     LGVFCGKYMTDCADEFPASWFKEAKLAPQNPDCSLNYFGVRASVPLRVWIEKGWIHPD
                     DPRGWFQWYCRYYSGRRMPDEDRRQIARWRAFRRHAAQVRKHCEPGDPFCRPKQRQAL
                     LQWAYDSRDI"
     gene            complement(9511..10554)
                     /locus_tag="VN034_03950"
     CDS             complement(9511..10554)
                     /locus_tag="VN034_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWV59781.1"
                     /translation="MIDQRTQIDFDTSVLPDRGFHSGEVWAGTQSKGPIPKAVASPLL
                     FVGTRADLPDMAVLSDIRVAGTLAASQAASHIRQTDTLDIIWLAAADAVESEMLADIC
                     AAAGEQGCNLICETSLSALDRIVAAVPASIEVQLLVDADAADKLVALAAARRTRYGAV
                     HDIARDDAMERIDRLQEEVARISRLLGDLAGQRSGLPGTPTGFVPWDADSAATEQVRA
                     PTRDYAAMPRSFVPEDRALDRQRAKAVRRMLRQRRMREQYFPADLFADPAWDMLLDLY
                     AARLERQPVSVSSLCIAAAVPATTALRWIKTMTDAGLFVREADPHDGRRIFIALAEGA
                     HDALARYFEALDE"
     gene            10860..11369
                     /locus_tag="VN034_03955"
     CDS             10860..11369
                     /locus_tag="VN034_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWV59782.1"
                     /translation="MLAGLALAAWVWLPAPATTVPLVHIIDGDSLMVRHDDSTLTIRL
                     TGIDAAEYRQDCARADGGRWSCGREARKMLEKLAGRGPLHCEVAAKDHYDRTLAACRT
                     RPAPDGIDLGAEMVRLGGAVATSDAYMVEEAEAQAQRRGIWQRDFVRPADWRATHERP
                     ANALAPPDS"
     gene            11432..11812
                     /gene="crcB"
                     /locus_tag="VN034_03960"
     CDS             11432..11812
                     /gene="crcB"
                     /locus_tag="VN034_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWV59783.1"
                     /translation="MNSLFPVMIGGAIGAGARHLVGQAMLARLGPGFPWWTLSINIAG
                     SLCMGLLIGALARGSDGGETARLFIGVGILGGFTTFSSFSMEFWMLFERGQIAQAAAY
                     MFASVIGAIVACGLGLFVMRQMPA"
     gene            11809..13041
                     /locus_tag="VN034_03965"
     CDS             11809..13041
                     /locus_tag="VN034_03965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWV59784.1"
                     /translation="MSAPDTKIDGATIAEEDDGIRLDRWFKRHRPGTPHALLARWARS
                     GQLTLDGKKADVSDRIETGQKLVMPTPPAEIEARPARKGRPLTEADVELAESMLIHRD
                     ASALVLNKLPGLATQGGTKTEQHVDGLLDALKYDAPVRPKLVHRLDKDTSGALLIARS
                     PRAAAYFAKSFSNRSARKTYWAIVVGVPDIAQGEIDLPLAKQPGSGGEKMHVHDSGLA
                     SKTRYRVIERAGNSAAWVELQPLTGRTHQLRVHMAAIGHPIVGDGKYGGKGAFLTGTI
                     SRKMHLHSRRLRIDHPDGGALDISAEVPEHFAASLDALGFDLLLGEVGIDDVAKGPPP
                     KAAVKAAAKAHSKQIRKARRGERRGRTADSKPTDFVGKPKPKAKAKPGKPGKPAPGKP
                     AGKKPSAKKHPARPPKAS"
     gene            13041..13661
                     /locus_tag="VN034_03970"
     CDS             13041..13661
                     /locus_tag="VN034_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWV59785.1"
                     /translation="MHPNSAFRPRQDDLAELLVREIGFAAIFAGTPDGPRVAHAPVVL
                     SDDRTTLQFHLARGNALTRHLDGASALAVVQGPDAYVSASWYTDADQVPTWNYVAIEM
                     EGVARRLDDSELVAQLDTLSAQHEARVGANPPWTRAKMNPAIFSKMTGAIVGFEMRIT
                     AWRPTIKLSQNKTVDERQRVIEGVEATGHGALAQLMRHLGGDREGA"
     gene            13658..13894
                     /locus_tag="VN034_03975"
     CDS             13658..13894
                     /locus_tag="VN034_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59786.1"
                     /translation="MSQADDALAKKRFFAITMMRLMGVAFVAIGFILIGGGFSFAGQP
                     ADRWIGVAIVLVGAFDFAVMPLLLARRWRSPKDL"
BASE COUNT         2482 a         4802 c         4367 g         2316 t
ORIGIN      
        1 gtcgacggtc accaagatca atgcgctcgt cgtgttcccg tttgaatcgg cggcgctgac
       61 caagcctgtc gcgcaggtca aggccaaggg tgcgtacgtc accgtggtcg accgcggcct
      121 gaccgacacc agcgcgcagg aaatgaccaa atggttcgac accaactatc atttcatggt
      181 ccccgaactg accgcgggcc agacgttcaa gatcgcttcg accaagatct tcgacgaatt
      241 tgaggaggcc aaggcgctgg gttatcaaac gcgccccgtc ctcctcggcc ccgtcagcta
      301 tctgatgctc gccaagggca aggcggtcga accgctcgcg ctgctgccgc agcttctcga
      361 cgcctacgcc gaaatcctcg cccgcctcgc caaggccggc gccgagtggg tccagatcga
      421 cgaaccctgc ctcgtcctcg acctttcggc cgagcagcgc accgccttcg aacgcgccta
      481 cgcccgcctc gctacccgcg ggccgaagct gatgctcacc acctactttg gtggtctggg
      541 cgacaatctg ggtctcgtcg ccgaccttcc cgtccacggc ctccacctcg acctcgtccg
      601 cgcgccgggc cagctcgaac ccgccctgac gaagctccag ccgcacgttc tgctctcgct
      661 cggcgtcgtc gacggccgca acatctggcg cgccaacctc cccgacctct attggcggct
      721 caaggaactc gccggcaaac gcgacctcat cctcgccccg tcctgctcgc tcctccacgt
      781 ccccgtcgac ctcgtgctcg aaacgtcgct cgaccccgaa atcgcgcagt ggctcgcctt
      841 cgcggcgcaa aagatcgaag agcttgccgc gctcgccaag gcgctgaacg aaggcgagga
      901 cgcgagcttc gccgccttct cggcctcgcg catcgccgcg gtcgcacgcc agacctcgcc
      961 aaaaatccac aaccccgcgg tcgccgcccg cctcgccgcg ctcgacgagt ccgccaccaa
     1021 ccgcgcctcg cccttcgccg accgacgcga cgcgcagcaa aagcgcctcg ccctccccgc
     1081 tttcccgacc acgacgatcg gcagcttccc gcagactccc gaggtccgca aggcccgcgc
     1141 cgcccacatc aagggcgaga tcgacgatgc cgcctacgac cgctatctgc gcgtcgaaac
     1201 gcgcgccgcg gtcaaatggc aggaggaaat cggcctcgac gtcctcgtcc acggcgagtt
     1261 cgaacgtaac gacatggtgc aatatttcgg cgagcagctc gccggcttcg ccttcacccg
     1321 cgccggctgg gtgcaaagct atggctcgcg ctgcgtccgc ccgccgatcc tctatggcga
     1381 cgtctcgcgc cccgcaccga tgaccgttgg ctggtggcgc tatgcgcagg accagaccga
     1441 ccgcccaatg aagggcatgc tcaccggccc cgtcaccatc ctcaactggt ccttcgtccg
     1501 cgacgaccag ccgcgcgcgc agagctgccg ccagatcgcg ctcgcgatcc gcgacgaggt
     1561 ggaggacctc gaggccgccg gcgccgcgat catccagatc gacgaggcgg ccttgcgcga
     1621 aggcctcccg ctccgccgct ccgaatggca gcattacctc gactgggcgg tcgaaagctt
     1681 ccgcatcgcc gccgcgggcg tccgcgacga cacgcagatt cacacgcata tgtgctattc
     1741 ggaattcaac gacatcatcc gcgcgatcgg cgacatggac gccgacgtca tctcgatcga
     1801 gaccgcgcgc tcgcggatgg agctgctcga cgccttcgcc agctacgcct atccgaacga
     1861 ggtcggcccc ggcgtctacg acatccacag cccgcgcatc cccgcaagtc aggagatgac
     1921 cgacctcctc accctcgccg caaaacatgt cccgcccgcg cagatatggg tgaaccccga
     1981 ctgcgggctc aagacgcgca aatgggacga ggttcgcccc gcgctcatag cgatggtcgc
     2041 cgcggcgaaa tcgatgcgcg cccgcgcgct cgtgaaggag gcctatcttg ccaaatgagg
     2101 ccatgccgcc cgaccggatg cccgaaggac agcccgccgt ccgcgtcacc gcgatgcccg
     2161 ccaacgccaa tgtctatggc gatattttcg gcggctggct gatggcgcag atggacctcg
     2221 ccgcctcgtc ggtcgcgtcg ctccacgcgc gcggccgcgc ggtgacgatt tcggtggagg
     2281 gcatgacctt ccaccgcccc gtcttcgtcg gcgacgaggt ctcggtcttc ggccgcctcg
     2341 tcaaggtcgg ccgcacttcg atgcacgtcg aagtcgaaac ctgggcgcgc gaccgccacg
     2401 ccgatgaagc gcacaaggtc acgcaggcgg tcttcatctt cgtcgcggtc ggccccgacc
     2461 gaaggccccg cgcggtgcgg cccttgacgg actgaatatg ggacgctcgc aaagggggtg
     2521 agggaacatc atctccccca cccccttgca tttccgtgac ctaggcgtaa ggcggcgcgg
     2581 acataaacct gcgcgtgcga ctccgcttag ctccgctctc ccgaacgggc accggccgtc
     2641 attccccgcc gcgccaacat ggggactgca atcctatgac caccaccggc cacgacacgc
     2701 tgggaacccg ctcgacgctc gacgtcggcg gcaaaagcta cgcttattat tcgctcgaca
     2761 aggccgcggc caagctcggc gacgtttcgc gcctgccctt ctcgatgaag gtgctgctcg
     2821 aaaatctgct gcggttcgag gatggcggct tcaccgtgtc gaaggacgac gtgcaggcgc
     2881 tcgtcgactg gcagaaggac ccgcattcga accgcgagat ccagtatcgc cccgcgcgcg
     2941 tgctgctcca ggatttcacc ggcgttccct gcgtcgtcga cctcgccgcg atgcgcgatg
     3001 cgatcgcgac gctcggcggc gacacgacga agatcaaccc gctcgtcccc gtccatctcg
     3061 tcatcgacca ttcggtgatg gtcgacgaat tcggccaccc caaggcgttc gagcagaatg
     3121 tcgagatcga atattatcgc aacggcgagc gctacgactt cctgaaatgg ggctcgaaga
     3181 gcctcgacaa tttcaaggcg gtgcccccgg gcaccggcat ctgtcaccag gtcaatctcg
     3241 aacatatcgc gcaggcggtg tggtcgagcg cggacggctc gggtgaaacc gtcgcctatc
     3301 ccgacacctg cgtcggcacc gacagccaca cgacgatgat caacggcctc ggcgtgctcg
     3361 gctggggcgt aggcggaatc gaggccgagg ccgcgatgct cggccagccc gtgtcgatgc
     3421 tcatccccga agtcgtcggc ttcaaattca ccggcgcgct gaaggaaggc gtgaccgcga
     3481 ccgacctcgt gctcaccgcg acgcagatgc tgcgcgagcg tggcgtcgtc ggccgcttcg
     3541 tcgaatattt cggccccggc ctcgcctcat tgagcctcgc cgaccgcgcg acgctcgcca
     3601 atatggcgcc cgaatatggc gcgacctgcg gcttcttcgg cgtcgacgac aagacgctcg
     3661 attacatgcg cctgaccggc cgcccggaag agaatatcgc gctggtcgag gcctatgcca
     3721 aggcgcaggg cctctggatc gtcgagggcg ccgcggaccc cattttcacc gacacgctcg
     3781 aactcgacct gtcgaccgtc gtcccctcgc tcgcggggcc gaagcggccg caggaccgcg
     3841 tctcgcttcc cgacgtcgac gatgtgttca acgccgacat ggcgaaggtc tataacaagg
     3901 cgcaggcgcg cgtgccggtc gagggcaagg atttcgacat cggcgacggc gacgtcacca
     3961 tcgccgcgat caccagctgc accaacacct cgaaccccgg cgtgctcgtc gccgcggggc
     4021 tcgtcgcgaa gaaggccgac gcgctggggc tgaagcccaa gccgtgggtc aagacctcgc
     4081 tcgcgccggg gtcgcaggtc gtcaccgact atctggagaa agccgggctg cagaagcatc
     4141 tcgacaatat cggcttcaac ctcgtcggtt atggctgcac gacctgcatc ggcaattcgg
     4201 gtccgctcgc cgagccgatt tcgaaggcga tcaacgaaaa tggcctcgtc gccgccgccg
     4261 tgatctcggg caaccgcaac ttcgaaggcc gcgtgtcgcc cgacgtgcgc gccaacttcc
     4321 tcgcctcgcc gccgctcgtc gtcgcttatg cgctcaaggg cacggtgatc gaggatttca
     4381 ccaccacccc gatcgggcag gaccagtcgg gtaacgacgt gttcctgaag gacatctggc
     4441 cgaccaatca ggaagtggcg gagacggttg cgggcgcggt cgaccgcgac atgttcgagg
     4501 cgcgctacgc tcacgtctac aagggcgacg agcattggca gaagatcgag gtcgagggca
     4561 gcgacaccta tcagtggcgc gccgggtcga cctatgtcgc gaacccgccc tatttcgagg
     4621 gcatgacgat gaccccggca cccgtgtcgg acatcgtgag cgccaagccg ctcgcgatcc
     4681 tcggcgacag catcaccacc gaccacatct ctccggcagg cagcatcaag gcggactcgc
     4741 cggccgggaa atggcttatg gaacatcagg ttagcaaggc cgacttcaac agctacggcg
     4801 cccgccgcgg ccaccacgac gtcatgatgc gcggcacctt cgccaacatc cgcatcaaga
     4861 acgaaatggt ccccggcatc gaaggcggca tgtcgcgcta tggcgccgag gtcatgccga
     4921 tctacgacgc cgcgatgcgc cacaaggccg acggcacgcc gctcgtcgtc atcgcgggca
     4981 aggaatatgg caccggctcg tcgcgcgact gggcggcgaa aggcaccaac ctgctcggcg
     5041 tccgcgcggt gatcgtcgaa agcttcgagc gcatccaccg ctcgaacctc gtcggcatgg
     5101 gcgtgctgcc gctgcagttc ctcgaaggcc agagccgcga gacgctggga ctgaccggcg
     5161 acgaccagtt caccatcacc ggcgttgccg atctgaagcc gcgccagacg gtgacggtga
     5221 acgtcacccg cgccgacggt tcgaccttca gcttcgacac gctctgccgc atcgataccg
     5281 cgaacgaggt cgaatattat atgaacggcg gcatcctgca ctatgtgctg cgcaaactcg
     5341 cggcctgacg ccgcgcaccg ctgatgctat gagcgcccgc cccctcaccg gggcgggcgt
     5401 tttcatatgg agaaaggccg catggacgcg gagaccatcg tcgtcgaggt tatcggctgg
     5461 accgccgccg cgatcatcct cgccgcctat atcctgctgt cgctcggcaa gctcgaaggg
     5521 cgcagctatt tctatcagtg gatgaacgtg atcggcgccg ggggcttcat cgtcaattcg
     5581 ggctacaacg gcgcgctgcc gtcggccgtg ctcaacgtca tatgggccgg gatgggattg
     5641 ttcacgctgt ggagcgtctg gcgcgcgcgg caagcggcgc gcgccgcaat cccttaacgc
     5701 gccgccgcct cggcgttcca cgccgcgacc ttcgcccgcg tatcggcgag cagcttgtcg
     5761 agcgccgcgc gatcctggac catcccgcgc atcaccaccg tatcgatagc gcgcgtcgcg
     5821 gcgatatcct cgagcggatt cttcgtcagc aacaccagat cggccgccat gcccggcttg
     5881 accgctccat aacgatcgag ctgcccgaac catgccggac cggcgcgcgt cgccgaagcg
     5941 agcgcttgcg ccggggtcag gcccttcgcc gtgaaaagct cgatctcgtc gtgcagcccg
     6001 aaaccgggat aattaaagct gttcaagaaa ccagcatcgg tccccgcgat gatcggtacc
     6061 cccgcttcgg cgagcatcgg caggatcgtg gcgacatcct cgatctgctt gtgccgtgcc
     6121 gcgatcgccg cggcgtcggc cttcgcggcg cgctcgatcc gccagtcata ggtcttgcgc
     6181 agcttcggcc cgatataggc gagcccctcg tccttcgaat gatcgtcgcg gtcgagatag
     6241 gcgatgatgc ggctgccgtt gagcgtcggg gtgacaaaca cgcccttcgc agcgaagcgg
     6301 cggtacgcag ccattgcagt atcgcggtcg aaaccggcat cgagccggcg gttcgcctcg
     6361 gcacggtcga tgcgcttcgc ggcgaaatcg gcggcgatcg cggcttcgtc cttcgcgccg
     6421 gcattatagg catagtcgag atgctcgatc gaactgatcc ccgcatcgac cgcctggtcg
     6481 acggtcagcg ccatcgggat gtgccccgac gtcttgaggc cgagggtctt tgcctgccgc
     6541 agcgcataga ggaagagctc gggcttcagc gtgctgtcgg tgatcttgac gaaatcgacc
     6601 ttgtcgcggt tcttcaggcg gtcgagcgcc gcatcgacat cggcctcgct gccgacctcg
     6661 atcgtgccct tccacaccgg ggcgattcct tcgatctttg cgcccgatgt cagcaagcgc
     6721 gggccgaaca ggctgccgcg cgcaatctcg ccgcgccagg cgagcacctg ctcgggcaga
     6781 tcccccgaac agtcgcggat cgtcgtgatt ccgttcgcaa tatagagcgg cagcagcgcc
     6841 ttattctctt cgatcaggtc gggcccgccg ccgaaatgga cgtgcatgtc ccaaaggccg
     6901 ggaatcagat agcgcccgtt gccctcgatc gttcgcgccg cgtgccagtc ttttgcaatc
     6961 gcgccatcgg cacctacggc gacaatatcg tcgccttgca gcaccaccgc ctgcccggcg
     7021 atcgtcttcg ccgcttcgac atcgacgatc gagacatgac ggatgatcac atcgccctgc
     7081 ttcggtgcgg cgagcgcgga cggggcaaaa gcgagcagtg ccggggcgaa tgtgaggaac
     7141 agttttttca tggggcgacc tttccgataa ctatcccctt aaccgcgtcg gacgcgagat
     7201 caagcgaccc agacgccctc gtccttgtgc cattcctcga tcgatttttc agggtaaagg
     7261 tcgaacgccg catcgccggg tccgatggcg ggttcgaccc agctcgcctt atatttgagc
     7321 atcagatgca cacgcgacgg cgccgccgga agctccgaat cgatcgcgct cgcgaagggg
     7381 tggacgagat cgggccagtt cgggtcgtaa agccacagtg ccgatccgca cccgcggcag
     7441 aagcgccgct caccgctgga cagcttgcat gacggatgct cgtcatcctc gatccgggcg
     7501 cgataaagac ccagatgctc ctcaccctcg atcgtcaggc tcgtcgcgtc ggcgccgagg
     7561 ttgatcgcat agcccccgcc gccctgttgc ttgcggcaga tcgaacaata acacagctga
     7621 tacggaacct gcgtatggct catcaccgaa aagcgcaccg cgccgcatcg gcacgagcca
     7681 tcgagtttgg tcggcatgga atgtcctttc gcgcgatcaa gggcccgcta gcgccaacgc
     7741 gcgatcgaaa ggaaggttcg tggtgggcgc tgacgggctc gaaccgccga ccctctcggt
     7801 gtaaacgaga tgctctacca actgagctaa gcgcccccaa agatgtcgcc atcagggagg
     7861 tgcgcgccct gccacaagcc gccgtcgctg gcaagcgatc ggtcgcccgc ggtcagagcc
     7921 agccgatatg tttgaagcgc caatagagcg tgaggcagat cgtgcccatc gcgccgacga
     7981 tcagcggata gccgaaggcc cagccgagtt cgggcatatg gtcgaaattc attccgtaaa
     8041 tgcccgcgat cgcggtcgga accgccagga tggcggccca ggcggcgagt tggcgggtga
     8101 tcacgccctg acgctgctgt tcgagcatgc cgttggtctc gatgaccgac gcggcaatgt
     8161 cgcgcagccc gttgaggcga tattcggcgc gctgcacatg atcccagacg tcgcgataga
     8221 aggggcgcac cgcggggtcg atattgggca gatcatcgtc ggtggtcagg cggccggcga
     8281 cctctttcat cattccgacc agccgctgga agcggatgat ctcgtgccgc tgcttgtaaa
     8341 ggtgacggat ttccttggcg tcgagcgggg tgtccatcac gctgtcctcg accgccagca
     8401 tccgttcctc gatcgcgtcg atcaccggga aatagccgtc gacgatgaaa tcgagcacgg
     8461 catagagaac ataatcgggc ccgtgcgcga gcttcgtcgg caggctttcg agccgcgccc
     8521 ggacatcggt gtgcgttcgc gccgatccgt ggcggacgct gacgaagaaa tgcgggccga
     8581 tgaaaaaagc ggtttcgccg ggctggatcg tatcgccatc gagattggcg gtacgggtga
     8641 tgatgaacag ctggtcgcca taggtttcga ccttgggcaa ctggttcgcc ttcagcgcgt
     8701 cctcgaccgc cagcggatgc aggccgaacc gcttggcgat gccggcaagc tcgcccgggg
     8761 tcggctcata gagaccaagc cagaagaaat cgccggtgtc gcaatcctcc gggatcgcct
     8821 cgtccggacc aaggtcgcgg acgagcttgc cgtcatgata gaggcgggcg gccatgatcg
     8881 gcattcggtg gaaactccct tgcgtgcggc gtctacatag tgcgcggggg cggcaaggga
     8941 aatcacctct taaacctgac tagatatccc tgctgtcata cgcccattgg agcagcgcct
     9001 ggcgttgttt cgggcggcaa aagggatcgc cgggctcgca atgcttgcgc acttgcgccg
     9061 catgacgccg gaacgcgcgc catcgcgcga tctgccggcg atcttcgtcc ggcatccgcc
     9121 gccccgaata atagcggcaa taccattgga accagccgcg cggatcgtcg ggatggatcc
     9181 agcccttttc gatccagacg cgaagcggca cgctcgcccg aacgccgaaa tagttcagcg
     9241 aacaatcggg attttgcggc gcgagctttg cctccttgaa ccagcttgca gggaattcgt
     9301 ccgcacagtc ggtcatatat ttcccgcaga acacgcccag cttcagcatt tccgccgggg
     9361 tgagttcggg ttcgaagtcg ggatcgaacg cccgccccat cggtgccgcg cgcgtgtaac
     9421 gataacccct ctgcatccgg tcgttcacaa ccacttcggc ttcgttcttc atcgggcgag
     9481 gctagcgccc cgctcccgga ccggccatcg ctattcgtca agtgcctcga aatagcgggc
     9541 gagcgcatca tgggctccct cggccagcgc gatgaagatg cggcggccgt cgtgcggatc
     9601 ggcctcgcgc acgaagaggc ccgcgtcggt catcgtcttg atccagcgca gcgccgtcgt
     9661 cgcgggaaca gcggccgcga tgcacaggct cgacaccgac accggctgac gttcgagccg
     9721 cgcggcgtaa aggtcgagca acatgtccca cgccggatcg gcgaacaggt cggcggggaa
     9781 atattgctcg cgcatccgcc gctgacgcag catccgccgc accgctttcg cacgctgccg
     9841 gtcgagcgcc cggtcttcgg gaacaaagct gcgtggcatt gccgcatagt cgcgcgtcgg
     9901 cgcgcgaacc tgctctgttg cggccgaatc cgcgtcccat ggcacgaagc cggtcggtgt
     9961 gccgggaagg ccgctccgct gtcccgcaag atcaccgaga aggcgggaaa tgcgcgcgac
    10021 ttcctcttgc agccggtcga tccgttccat cgcatcgtcg cgggcgatat cgtggaccgc
    10081 cccgtaccgc gtgcgtcggg ccgccgcgag cgcgacgagc ttgtcggccg catcggcatc
    10141 gacaagcaat tggacctcga tagatgcggg aacggccgcg acgatccggt cgagcgccga
    10201 caaagaggtt tcgcaaatca ggttgcatcc ctgctcgccc gctgccgcgc aaatatcggc
    10261 cagcatctcg ctctccaccg cgtccgctgc cgccagccag atgatatcga gcgtatcggt
    10321 ctgccggata tggctcgccg cctgcgacgc cgcgagcgtt ccggccaccc gtatgtcgga
    10381 gaggacagcc atatcgggca aatccgcgcg ggtcccgacg aacagcagcg gcgacgcgac
    10441 agctttgggg atcgggccct tggattgtgt ccctgcccag acctcacccg aatgaaaacc
    10501 ccgatccgga agaaccgacg tgtcgaagtc gatttgcgtc ctctggtcga tcatgctgca
    10561 gcccccgttt ccatcgctcc accttggcgt aactatgtcg tcattccggc gtcattcacc
    10621 ttttgttcgt tcctgtttgc cgccaatccg gacccaagcg cgccattggc atgagtcgcc
    10681 actaggtcgg ctggaacgga atgatccatt ttggacatga tttgttcttc ccgtcaatat
    10741 tttattgaga cttgccaacc cggccgccgc gggcgcactc ggggaccatg atccgcgctt
    10801 tgtctcccct cacccgcttg cgctggcggc gacggctgcg ttcgctcatc gcgttgctgt
    10861 tgctcgcagg cttggcgctg gcagcgtggg tgtggttacc ggcacctgcc acgacggtgc
    10921 cgcttgtcca tatcatcgat ggcgacagcc tgatggtgcg gcatgacgac agcacgctga
    10981 ccattcgcct gaccgggatc gacgcggccg aatatcggca ggattgcgcg cgcgccgacg
    11041 ggggccggtg gtcctgcggc cgcgaggcac gaaagatgct cgaaaagctc gcgggccgcg
    11101 ggccgctcca ttgcgaagtc gcggcgaagg atcattatga tcgcaccctc gccgcttgcc
    11161 gtacccggcc cgctcccgac gggatcgatc tgggcgccga gatggtccgg ctgggcggcg
    11221 cggtcgcgac cagcgacgcc tatatggtcg aagaggccga agcgcaggcg cagcggcgcg
    11281 ggatctggca acgcgatttt gtccggcccg ccgactggcg cgcaacgcac gaacgccccg
    11341 cgaatgcgct cgcgccgccc gacagctaat atcaggcgca ccatgacgca cccgctatgc
    11401 ctcgccgccg tttccggcta gggcgagcga catgaacagc ctgtttcccg tcatgatcgg
    11461 cggcgcgatc ggcgccggcg cgcgccatct ggtggggcag gccatgctcg cgcggctcgg
    11521 ccccggcttt ccctggtgga cgctgtcgat caacatcgcg ggcagcctct gcatgggcct
    11581 gctgatcggc gcgctggcgc ggggcagcga cggcggcgaa accgcgcgcc tcttcattgg
    11641 ggtcggcatc ctcggcggct tcaccacttt ttcttctttc agcatggaat tctggatgct
    11701 tttcgaacgc ggtcagatcg ctcaggcggc cgcctatatg ttcgcgtcgg tgatcggcgc
    11761 gattgtcgcc tgcgggctcg gcctgttcgt catgcggcag atgccggcat gagcgcgccc
    11821 gacaccaaaa tcgacggcgc gacgatcgcc gaggaggatg acggcatccg tctcgaccgc
    11881 tggttcaagc gccatcggcc ggggacgccg cacgccttgc tcgcgcgctg ggcgcgctcg
    11941 ggacagctga cgctcgacgg caagaaggcc gacgtgtcgg atcgcatcga aacggggcaa
    12001 aagctcgtca tgccgacccc gcccgccgag atcgaagcac gcccggcgcg caagggtcgt
    12061 ccgctcaccg aagccgatgt cgaactcgcc gaatcgatgc tcatccaccg cgacgcgagc
    12121 gcgctcgtgc tcaacaagct gcccggcctc gcgacgcagg gcggcaccaa gaccgagcag
    12181 catgtcgacg gcctgctcga cgcgctcaaa tatgacgcgc cggtgcggcc caagctcgtc
    12241 caccggctcg acaaggacac gtcgggcgcg ctgctgatcg cgcgaagccc acgcgccgcc
    12301 gcttatttcg cgaagagctt ttcgaaccgc agcgccagga agacctattg ggcgatcgtc
    12361 gtcggcgtgc cggacatcgc acagggcgaa atcgacctgc cgctcgccaa acagccagga
    12421 tcgggcggcg agaagatgca cgtccacgac agcggcctcg cgtcgaagac gcgctaccgc
    12481 gtgatcgagc gcgcggggaa tagtgcggcg tgggtcgaac tccagccgct gaccgggcgc
    12541 acgcaccagc tgcgcgtcca tatggcggcg atcggccatc cgatcgtcgg cgacggcaaa
    12601 tatggcggca agggcgcctt cctgaccggg acgatcagcc gcaagatgca cctgcacagc
    12661 cggcggctgc gcatcgacca tcccgacggc ggcgcgctcg acatcagcgc cgaggtgccc
    12721 gagcatttcg ccgccagcct cgacgcgctg ggcttcgacc tgctgctcgg cgaggtcgga
    12781 atcgacgatg tcgccaaggg cccgccgccg aaggccgcag tcaaggcggc cgccaaggcg
    12841 cacagcaagc agatccgcaa ggcgcggcgc ggcgagcggc gcggacgcac cgccgacagc
    12901 aagccgaccg acttcgtcgg caagccgaaa cccaaggcga aagcgaaacc gggcaagccc
    12961 ggcaagccgg cgcccggaaa acccgcgggc aagaaaccga gcgccaaaaa gcatccggcg
    13021 cgcccaccca aagcgagctg atgcacccga actccgcctt ccggccacgg caggacgatc
    13081 tcgccgaatt gctcgtgcgc gagatcggtt tcgccgcgat cttcgcgggc acccccgacg
    13141 gaccacgcgt cgcgcacgct ccggtggtgc tgagcgatga caggaccact ctgcaattcc
    13201 acctcgcgcg cggtaacgca ctcacacgcc acctcgacgg cgcgagcgcg ctcgcggtcg
    13261 tgcaaggccc agatgcctat gtcagcgcca gttggtatac cgacgccgac caggtgccga
    13321 cgtggaatta tgtcgcgatc gagatggaag gtgtcgcccg caggctcgac gacagcgagc
    13381 tggtcgcgca gctcgacacc ctctcggcac agcatgaagc acgcgtcggg gcgaatccgc
    13441 cgtggacgcg cgccaagatg aatcccgcga tcttcagcaa gatgaccggc gcgattgtcg
    13501 gcttcgagat gcgcatcacc gcttggcgcc cgacgatcaa gctgtcgcag aacaaaacgg
    13561 tcgacgagcg ccagcgcgtg atcgagggcg tcgaggcgac cggccacggc gcgctcgcgc
    13621 agttgatgcg ccacctcggc ggcgataggg agggcgcatg agccaggccg acgacgcact
    13681 cgccaagaag cgttttttcg cgatcaccat gatgcgcctg atgggcgtcg ctttcgtggc
    13741 gatcggcttc atcctgatcg gcggcggctt ttcgttcgcg gggcagcccg ccgaccgctg
    13801 gatcggcgtt gccatcgtac tcgtcggcgc gttcgatttc gcggtcatgc ccttgctcct
    13861 cgcgcgccgg tggcgctccc ccaaagacct gtgaagcgat tctggaaaga tgttgccgtc
    13921 gccgccgagg gtgacggctg gggcatcgcg ctcgacgggc gatcggt
//
LOCUS       DATMSU010000147         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_173291,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000147 DATMSU010000000
VERSION     DATMSU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173291"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2079
                     /locus_tag="VN034_03980"
     CDS             <1..2079
                     /locus_tag="VN034_03980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWV59787.1"
                     /translation="ATPAPAPAGANPLLQPWTGPFEGVPPWDKLDPELFPDAFTKGMA
                     ETKAEVQAVIDNPEAPTFENTHVPMMLAGDTMERLFAMWGVQTSNKSSDRVEDIDAEW
                     SPKLTKFYTELFLDPKLFARYKAVYDKRNSSGLDAQQIRIVERSYDEMVRDGANLSPA
                     DKVKLVEMNSKLEGLFSAFSSKLLGDEKLYTFVTDKTELAGLESGFVASLAAAAEANG
                     KPGQWAIKNTRSSAQPVLQNATNRALREKVYKAFVNRGDNGDANDTNATIVEILKLRQ
                     QRAELLGFPTHAHYRMADTMAKTPEAAMGLMMKVWPAAVARVKEEVADMQAIADAEAK
                     ANKTPKITIEPWDYRFYAEKVRKAKYDLDESEVKPYLQLDKLTQGMFWAAGQLYDLGF
                     KENTGTIPVFDPKVRTFEVYNLKTNENVGVFYLDNFARDGKRSGAWMTTYRSQQTLGG
                     ERNVLASNNNNFTEAAKGEPTLISLDDAQTLFHEFGHAIHYLLQQVTYPALAGVPRDF
                     VEYPSQVNENWLMTPEVLSKYATHYKTGEPMPQALVDKILASDKFNQGFTTVEYLASA
                     IVDMKLHDRKTPPADADKFERETLAEIGMPKEIVMRHRLPQFGHLFSSDAYSAGYYSY
                     LWSETMDADTWAAFTEAGGPWDRSVADKFRTILLMTGNETDRAEAYRAFRGRDPDVKA
                     LLAKRGFPTE"
     gene            2313..3521
                     /gene="manD"
                     /locus_tag="VN034_03985"
     CDS             2313..3521
                     /gene="manD"
                     /locus_tag="VN034_03985"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HWV59788.1"
                     /translation="MKITAARVIVTCPGRNFVTLKIETDQGVHGIGDATLNGRELAVV
                     SYLQDHVAPCLIDMDPRRIEDIWQYLYRGAYWRRGPVTMRAIAAVDVALWDIKAKMAG
                     MPLYQLLGGRSRDGVMVYGHANGADIAETVDAVGQYIDLGYKAIRAQTGVPGIKDAYG
                     VGRGKLYYEPADAALPSVTGWDTRKALNHVPRLFETLRATYGFDHHLLHDGHHRYTPQ
                     EAANLGKMLEPYQLFWLEDVTPAENQEAFKLVRQHTVTPLAVGEIFNSIWDAKELIEN
                     QLIDYIRTTIVGAGGVTHLRRIADLASLYQIRTGCHGATDLSPVTMGTALHFDTWVPN
                     FGIQEYMRHSAETDAVFPHDYHFADGFLYCGETPGHGVDIDEELAARYPYKPACLPVA
                     RLEDGTMWNW"
     gene            3509..>3787
                     /locus_tag="VN034_03990"
     CDS             3509..>3787
                     /locus_tag="VN034_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59789.1"
                     /translation="MELVSGMAITRRSLLRGSAVAAALPVSAVGATAAMAPIERLPIW
                     PGPPPGGAGLRVRDEVIKRSPDGPADDIAWPHVATPMLNVVPAARLNGG"
BASE COUNT          761 a         1208 c         1192 g          626 t
ORIGIN      
        1 gccacgccgg cccccgcgcc cgcgggcgcg aacccgctgc tccagccttg gaccggcccg
       61 ttcgaggggg tgccgccgtg ggacaagctc gaccccgagc tgttccccga cgccttcacc
      121 aagggcatgg ccgagaccaa ggccgaggtg caggcggtga tcgacaatcc cgaagcgccg
      181 accttcgaga atacgcatgt gccgatgatg ctcgcgggcg acacgatgga gcgcctcttc
      241 gcgatgtggg gcgtgcagac gtcgaacaag tcgagcgacc gcgtcgagga tatcgacgcc
      301 gaatggagcc cgaagctcac caaattctat accgagctgt tcctcgatcc caagctcttc
      361 gcgcgttaca aggcggttta cgacaagcgc aattcgagcg ggctcgacgc gcagcagatc
      421 cgcatcgtcg agcgcagcta tgacgagatg gtccgcgacg gcgccaacct gtcgcccgcc
      481 gacaaggtga agctggtcga gatgaactcg aagctcgaag gtctgttctc ggccttctcg
      541 tcgaagctgc tcggcgacga gaagctctat accttcgtca ccgacaagac cgagctcgcg
      601 gggctcgaat ccggcttcgt cgcctcgctc gccgccgccg ccgaagccaa tggcaagccc
      661 ggccagtggg cgatcaagaa cacgcgatcg tcggcgcagc cggtgctgca gaatgcgacc
      721 aaccgcgcgc ttcgcgaaaa ggtctacaag gcgttcgtga accgcggcga caatggcgac
      781 gcgaacgaca ccaatgcgac gatcgtcgaa atattgaagc tgcgccagca gcgcgccgaa
      841 ttgctcggtt tcccgaccca cgcgcattac cgcatggccg atacgatggc gaagaccccc
      901 gaagccgcga tggggctgat gatgaaggtc tggcccgccg cggtcgcgcg ggtgaaggaa
      961 gaggtcgccg acatgcaggc gatcgccgat gccgaagcga aggcgaacaa gacgccgaag
     1021 atcaccatcg agccgtggga ttaccgtttt tacgccgaaa aggttcgcaa ggcgaaatac
     1081 gacctcgatg aaagcgaagt gaagccctat ctccagctcg acaagctgac ccagggcatg
     1141 ttctgggcgg cggggcagct ctacgacctc ggtttcaagg aaaataccgg cacgatcccg
     1201 gtgttcgacc ccaaggtccg caccttcgag gtctataacc tcaagacgaa cgagaatgtc
     1261 ggggtcttct atctcgacaa tttcgcgcgc gacggcaaac gctcgggcgc gtggatgacg
     1321 acctatcgca gccagcagac gctcggcggc gagcgcaacg tcctcgcctc aaacaacaat
     1381 aatttcaccg aagccgcgaa gggcgagccg acgctgatca gcctcgacga tgcgcagacc
     1441 ttgttccacg aattcggcca cgcgatccat tatctgctgc agcaggtcac ctatccggcg
     1501 ctggcagggg tgccgcgcga cttcgtcgaa tatccgagcc aggtgaacga gaattggctg
     1561 atgacccccg aagtgctgtc gaaatatgcg acgcactaca agacgggcga gccgatgccg
     1621 caggcgctgg tcgacaagat cctcgcctcc gacaagttca accagggctt caccacggtc
     1681 gaatatctgg cgagcgcgat cgtcgacatg aagctgcacg accgcaagac gccgccggcc
     1741 gacgccgaca agttcgagcg cgagacgctg gccgaaatcg gcatgccgaa ggaaatcgtc
     1801 atgcggcacc gcctgccgca gttcgggcat ctgttctcga gcgacgccta ttcggcgggc
     1861 tattacagct acctctggtc ggaaacgatg gacgccgaca cctgggcggc cttcaccgaa
     1921 gcgggcgggc cgtgggatcg cagcgttgcc gacaagttcc gcaccatcct gctgatgacc
     1981 ggcaacgaga ccgaccgcgc cgaggcctat cgcgccttcc gcggccgcga tcccgatgtg
     2041 aaggcgttgc tcgcgaagcg cggttttccg accgagtaag gcgagcgaca cccacgcggg
     2101 caaaggggcg cgacaaaggg ggcgatttcg ccccctttgt ttttggggcg catttgcacc
     2161 gtgaacgagc cattccgatc agcgggaaac agccggtcgg gatcgttttt caaggatcgc
     2221 aaaacgccca ttttcgtagt ggccttacca atattgttga tattggcaag ggtgatgcta
     2281 agcacgatgt cccatccatt tggaacattg tcatgaaaat cactgccgcc cgtgtcatcg
     2341 tcacctgccc cgggcgaaac ttcgtcacgc tcaagatcga aaccgaccaa ggcgtccacg
     2401 gcatcggcga cgcgacgctg aacgggcgcg agctggcggt cgtgtcctat ctccaggatc
     2461 atgtcgcacc gtgcctgatc gacatggatc cgcgccggat cgaggatatc tggcaatatc
     2521 tctatcgcgg cgcctattgg cggcgcggcc cggtgacgat gcgcgcgatt gccgcggtcg
     2581 atgtggcgct gtgggatatc aaggccaaga tggcggggat gccgctctat cagttgctgg
     2641 gcgggcgtag ccgcgatggc gtgatggttt atggtcatgc caatggcgcg gacatcgcgg
     2701 aaaccgtcga cgcggtcggt cagtatatcg acctcggcta caaggcgatc cgcgcccaga
     2761 ccggcgttcc ggggatcaag gacgcctatg gagtcgggcg cggcaagctc tattacgaac
     2821 ccgccgatgc cgcgttgccc agcgtaaccg gctgggacac gcggaaggcg ctaaaccatg
     2881 tccccaggct gttcgagacg cttcgcgcca cctatggctt tgaccatcac ctgctgcacg
     2941 acggccatca ccgctatacg ccgcaggaag ccgccaatct cggcaagatg ctcgaaccct
     3001 accagctgtt ctggctggaa gacgtcaccc cggcggagaa tcaggaggcg ttcaagctgg
     3061 tccgccagca cacggtgact ccgctggcgg tgggcgaaat cttcaactcg atctgggacg
     3121 ccaaggagct gatcgagaac cagctgatcg actatatccg cacgaccatc gtcggcgcgg
     3181 gcggggtcac ccatctgcgg cggatcgccg acctcgcgag cctttaccag atccgcaccg
     3241 gctgccacgg cgcgaccgac ctgtcgccgg tgacgatggg caccgcgctg catttcgaca
     3301 cttgggtccc caatttcggt attcaggaat atatgcggca cagcgcggag accgacgcgg
     3361 tcttcccgca cgattatcat tttgcggacg gcttcctcta ttgcggcgag acgccgggcc
     3421 acggcgtcga catcgacgag gaactggccg cgcgataccc ctataagccc gcctgtcttc
     3481 ccgtcgcccg tctggaagac gggacgatgt ggaactggtg agcggcatgg cgatcacgcg
     3541 caggagcctg ctcagggggt cggccgtcgc ggcggcgctg ccggtttccg ccgtcggagc
     3601 cacggcagcg atggcaccga ttgagcggct tccgatctgg ccgggcccgc cgcccggtgg
     3661 cgcggggctg cgcgtacgcg acgaggtgat caaacgatcg cccgacgggc ccgccgacga
     3721 tatcgcctgg ccgcacgtcg cgacgccgat gctgaacgtc gtgcccgcag ccagactgaa
     3781 cggcggc
//
LOCUS       DATMSU010000148         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_173311,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000148 DATMSU010000000
VERSION     DATMSU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173311"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1067)
                     /locus_tag="VN034_03995"
     CDS             complement(243..1067)
                     /locus_tag="VN034_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV59790.1"
                     /translation="MSANESDPGLSACLTDREMEILRLLVAGHTVKTIAARLGRSETS
                     INERLRSARRKTGVGSSRELARLLDRQKTCDENIDLSSQRSTVKGLAHTATIGVRGSK
                     GMIVMLIAIPLAAAGLMVATAPSADQAGTADAVHSAASRQLPLAGSWSLDASAMPEKE
                     RPQRVTMTFRASQDGKWTTRVEIVAPDGSRRHAESTAALDGVAVPVTGNMDFIDSVAL
                     RQPAPDTLVMTLGKAGTRVSTRVYTVAKDLKSMTETIVWSSDKLQRLETTRFNRIS"
     gene            complement(1636..2961)
                     /locus_tag="VN034_04000"
     CDS             complement(1636..2961)
                     /locus_tag="VN034_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV59791.1"
                     /translation="MRILRTGAAALALTVSGAMPAAAQALRGEVEQQMPSLMAIYKDL
                     HAHPELSFMEVRSAGILAAEARKLGFKVTEKVGGTGIVAVMENGAGPVVMVRADMDGL
                     PVTEQTGLPGASKVRVTTKEGVETGVMHACGHDTHMTAWVGVARLMAANKDKWSGTLV
                     MIGQPAEERGAGARMMLEDGLYTRFPKPEYVLAFHDAAQFPAGKIGYTPGYALANVDS
                     VDILVKGVGGHGAYPQATKDPIVLGSRIVGALQTLVSREVSPLDSAVVTVGSFHAGAK
                     HNIISDEARLQLTVRSYTDAVRDHLLDGIARIAKGEAIAAGVPEDRMPVVTVQRDEYT
                     PATFNTPEFTEEMAAYLKTSFGEDRVVKMPPVMGGEDFGRFGREDKSIKSLIVWVGGV
                     PQAEYDAAKKEGRTLPSLHSPFWAPDAPAVISTATEALTAMTMKLMGKK"
     gene            complement(2987..>3614)
                     /locus_tag="VN034_04005"
     CDS             complement(2987..>3614)
                     /locus_tag="VN034_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HWV59792.1"
                     /translation="VLAVALGATLSACKTVPAVPPSAADAPADATGTWRATATDADKE
                     RIRGWYSSWQAALADARAKGFGADINREGVLLQPMAALPNPHLPAGDYRCRTVKVGAK
                     GRSTLAYVGYSWFRCRVAPEQGLSSLTKLTGSQRPVGLIFPDNLKRQVFLGTLELGDE
                     KMAVNYGSDRLRDMAGLVERIGDNRWRLVLPAPAYESLLDVIELVPAG"
BASE COUNT          605 a         1233 c         1106 g          670 t
ORIGIN      
        1 aacccctcct cttcagagga ggggcagtga gacttggtcc ggcgcagccg ggcctttagt
       61 cgaacggggt ggtggtggcg gcacgcgcac gcctagccga gaccaccacc ccgctgcgac
      121 taacgaacaa gttcgtaagt ctcgctaccc ctcctctgaa gaggaggggc tggcgcatct
      181 ctgccggacg gcagcttccg gtcgattccg gtcatttgac ccagatcggc accaatgacc
      241 aatcaggaaa ttcgattaaa acgcgtcgtt tccagccttt gaagcttgtc gctcgaccac
      301 acaatcgttt cggtcatgga tttcagatcc ttggcgacgg tatagacgcg cgtcgacacc
      361 cgcgtgcccg cctttcccaa cgtcatcacc aatgtgtccg gcgccggctg acgcagcgcg
      421 acactgtcga tgaaatccat attgccggtg accggtaccg cgacgccatc gagcgcggcg
      481 gtcgattccg catgccggcg ggagccgtcg ggtgcgacga tttcgacccg cgttgtccat
      541 ttgccgtcct gcgatgcgcg gaacgtcatc gtcacccgtt gcggccgttc tttctcgggc
      601 atggccgatg cgtccagcga ccagcttccg gccagcggca gctgccggga tgcggcgctg
      661 tggacggcgt cggctgttcc ggcttgatcg gccgaagggg cggttgcgac catcaatcca
      721 gcggccgcca ggggtattgc aatgagcatg acgatcattc ctttcgaacc gcgaacccca
      781 atggttgcgg tgtgcgccaa acctttcacg gtggagcgct ggctcgaaag atcgatattt
      841 tcgtcacaag ttttttggcg gtccagaagc cgcgccaatt cgcggctgct gccgacgccc
      901 gtcttgcggc gcgcggagcg caaacgctcg ttaatcgagg tttccgaacg gcccagacgc
      961 gcggcgatgg tcttgacggt gtgcccggcg accaacaggc gcaaaatttc catttcccgg
     1021 tcggtaaggc atgcggacaa gccgggatca gattcattgg cagacatgtc tttcgctcac
     1081 cacagcccca cgaacaggtc catcccaatt taattggaaa acggccgcgg gaccagaccg
     1141 ggcgtgagag aagcggtggg gctcgctgat cagttcatcc cgtctgacgg atcaataata
     1201 tccgaaattc aggaaagacc agcctcatgc acctcccgat ttgcgccgtg cgacgcggtg
     1261 gaggcgcacc gccgtggcga cttccacaat ccaggtcgcg atcgtttttg accaccaatt
     1321 gaacgagccg ggatgtaggc tggcatagcc agatattgta ggaaaggcct tttacagcgc
     1381 gatggctgct ttggggtggt gagcggacgt ttccccaaac ccctcctctt cagaggaggg
     1441 gcagtgagac ttggttcggc gcagccgggc ctttagtcga acggggtggt ggtggcggca
     1501 cgcgcgcgcc tggccacgac caccaccccg ctgcgactaa cgaacaagtt cgtaagtctc
     1561 gctacccctc ctctgaagag gaggggctgg ctcatgcttg ccaacgtctc ctgacggtcg
     1621 tcagcggacg atcccttatt tcttccccat cagcttcatc gtcatcgcgg tcagcgcctc
     1681 ggtcgcggtc gagatcaccg cgggcgcgtc gggcgcccag aagggcgagt gcaggctcgg
     1741 caatgtccgg ccttccttct tcgccgcgtc atattcggcc tgcgggacgc cgccgaccca
     1801 gacgatcagg ctcttgatgc tcttgtcctc gcgcccgaag cggccgaaat cctcgccgcc
     1861 catcaccggc ggcatcttga cgacgcgatc ctcgccgaag ctcgtcttca gatacgccgc
     1921 catttcctcg gtgaattcgg gcgtgttgaa ggtcgcgggc gtatattcgt cgcgctgcac
     1981 cgtcaccacg ggcatccggt cctcgggtac gcccgccgcg atcgcctcgc ccttcgcgat
     2041 gcgcgcgata ccgtcgagca gatggtcgcg caccgcgtcg gtatagctgc gcaccgtcag
     2101 ctgcagccgc gcttcgtccg aaatgatatt atgcttcgcg cccgcatgaa agctgccgac
     2161 cgtcaccacc gcgctgtcaa gcgggctcac ctcgcgcgac accaaggtct gcagcgcgcc
     2221 gacgatccgg ctccccagca cgatcgggtc cttcgtcgcc tgcggatagg cgccgtggcc
     2281 gccgacgccc ttcaccaata tgtcgacgct gtcgacgttg gcgagcgcat agcccggcgt
     2341 atagccgatc ttgcccgcgg gaaactgcgc cgcgtcgtgg aaggcgagca catattcggg
     2401 tttcgggaag cgcgtataaa gcccgtcctc gagcatcatc cgcgcgcccg cgccgcgctc
     2461 ctcggcgggc tggccgatca tcaccagcgt gcccgaccat ttatccttgt tcgccgccat
     2521 cagccgcgcg acgccgaccc acgcggtcat atgcgtatcg tggccgcacg cgtgcatgac
     2581 gccggtttcg acgccttcct tggtggtgac gcgcaccttc gacgcgccgg gcaggcccgt
     2641 ctgctcggtc accggcaggc cgtccatgtc ggcgcggacc atcacgaccg ggccggcgcc
     2701 gttctccatc accgcgacga tgccggtgcc gccgaccttc tcggtgacct tgaagcccag
     2761 cttgcgcgcc tcggcggcga ggatgcccgc cgagcggact tccatgaagc tgagttcggg
     2821 atgggcgtgc aaatccttgt agatcgccat cagcgacggc atctgctgtt cgacctcgcc
     2881 gcgcagcgcc tgcgccgccg cgggcatcgc gcccgatacc gtcaaggcca gcgccgccgc
     2941 gcccgtccgc aaaatccgca tcgtctttcc cctgtttgtc tgttcttcag cccgccggga
     3001 ccagttcgat cacgtcgagc agcgattcat acgccggcgc gggcagcacg agccgccagc
     3061 gattgtcgcc gatccgttcg accagccccg ccatgtcgcg caggcggtcg ctgccataat
     3121 tgaccgccat cttctcgtcg ccgagttcca gcgtgccgag gaagacctgc cgcttcagat
     3181 tgtcgggaaa gatcagcccg acgggacgct gcgacccggt cagcttcgtc aggctcgaca
     3241 atccctgttc gggcgcgacg cggcagcgga accagctgta accgacatag gccagcgtac
     3301 tgcgcccctt cgcccccacc ttgaccgtcc ggcaccgata atcgcccgcg ggcaggtgcg
     3361 gattgggcaa agccgccatc ggctggagca gcacgccttc gcggttgatg tcggcgccga
     3421 accccttggc gcgcgcatcg gccagcgcgg cctgccagct gctgtaccag ccgcggatgc
     3481 gttccttgtc ggcgtcggtc gccgtcgcgc gccaagttcc cgtcgcgtcc gccggcgcat
     3541 cggcggcaga cggcggaacc gccggtacgg tcttgcaagc actcagggtg gcgccgagcg
     3601 ccaccgccaa aacc
//
LOCUS       DATMSU010000149         3549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_163629,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000149 DATMSU010000000
VERSION     DATMSU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163629"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..2217)
                     /locus_tag="VN034_04010"
     CDS             complement(91..2217)
                     /locus_tag="VN034_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59793.1"
                     /translation="MRLLCSAGFSLALFLAAPAFAQDRANAGGDDDVHTGEPIIVTAP
                     YVRSLDILGNVSVLEGDELARDIRGQIGDTLTRQPGVSATSFSPGASRPVLRGFSGER
                     VKILTDGIGSIDASNTSADHAVTIDPLTVERIEILRGPAVLLFGSQAIGGAVNMFDRR
                     IPRKVPADHVHVDAIGGYATAANDRNIGSSIDVALSPQIVAHLDGSWRKTGDTRSGGF
                     VYAPDIRGDLLHLAEHEVEEGHLDEAAELTEQAGKRGKIDNTQSETWTAGGGISLIND
                     GGQLGISVGYFDTNYGVPARPNTAHDHGGEEEEGGHDHGEGPVTIGMKQWRADLRGEV
                     ELGDGFFDKLRIRAGFADYKHTEFEGDEVGTIFTNQGVEGRLELAQNDRGGWRGASGV
                     QYSHRDFNAVGAEAFVPRNLTDQFALFTLQEYTAGPLGLEAAARYEKTNVRAQTLGFE
                     RNFDSFSGALGATYDIFEGAKFGVSVSRAVRAPSAEELLSNGPHIATQSFEVGDPNLK
                     RESNWGAEASFKFKSDGFNLSLTGYSNWFDNFIYSAATGAEEDELPVFQYFQRDARVY
                     GFEAEASARLAQIGGFNIVGDVTADMTRAKIKNAGLDRNVPRIPPLRVLGGLEAQGER
                     VDARVEVEWTDDQTRVAQFETPTDGFTLVNASLSWRPLPDTKNLTLMLSANNIFDVEA
                     RRHASFTKDYVPLAGRDFRLTARASF"
     gene            2410..3387
                     /locus_tag="VN034_04015"
     CDS             2410..3387
                     /locus_tag="VN034_04015"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HWV59794.1"
                     /translation="MTIAANSLDLIGNTPLVLLKGPSEATGCEIWGKCEYANPGGSVK
                     DRAALYIVRDAEANGSLRPGGTIVEGTAGNTGIGLALVANALGYRTIIVMPDNQSAEK
                     MATIRALGAELVLVPPAPFANPGHFVHTSRRIAEETDNAIWANQFDNIANRKAHIFGT
                     AEEIWEQMDGRIDGFTCAAGTGGTIAGTGLGLKAHDPDIVIALTDPHGAGLYNYYQCG
                     ELKPEGSSVAEGIGQNRITANLEGAPIDTQFRISDAEGLAVVRRLLDEEGLCLGLSSG
                     INVAGAMALARQLGPGKRIATILCDSGFRYLSTLYNPEWLASKGLEAAA"
BASE COUNT          609 a         1158 c         1180 g          602 t
ORIGIN      
        1 cgtcccggtt tagtgcaccg gcgagatccc ggccttcgcc gggatgacaa gatatagaga
       61 ggcggcgttt cagcccaacc cgatcacgct ttagaagctc gcccgcgccg tcaaacggaa
      121 atcgcgcccc gcgagcggca cataatcctt ggtaaagctc gcgtggcggc gcgcttcgac
      181 atcgaagata ttgttcgccg acagcatcag cgtcagattc ttcgtgtcgg gaagcgggcg
      241 ccagctgagc gaggcgttga ccaaggtaaa gccgtcggtc ggcgtctcga actgcgcgac
      301 gcgcgtctgg tcgtcggtcc attcgacctc gacgcgcgca tcgacgcgct cgccctgcgc
      361 ctcgagcccg cccagcacgc gcagcggcgg gatgcgcggc acattccggt cgagacccgc
      421 attcttgatc ttcgcgcgcg tcatgtcggc ggtgacgtcg ccgacgatgt tgaagccgcc
      481 gatctgggcc agccgcgcgc tcgcctcggc ctcgaaacca taaacgcgcg cgtcgcgctg
      541 aaaatattgg aagacgggca gctcatcctc ttcggcgccg gtggcggccg aatagatgaa
      601 attgtcgaac cagttcgaat agccggtgag gctgaggttg aagccgtcgg acttgaattt
      661 gaacgaggct tcggcgcccc agttcgattc gcgcttcaaa ttcgggtcgc cgacctcgaa
      721 gctctgcgtc gcgatgtgcg ggccgttcga cagcaattcc tcggccgacg gcgcacgcac
      781 cgcgcgcgac accgaaacgc caaacttcgc gccttcgaag atatcatagg tcgcgccgag
      841 tgcgcccgag aagctgtcga aattgcgctc gaagcctagc gtttgcgcgc ggacattggt
      901 cttttcgtaa cgcgccgcgg cttcgaggcc gagcgggccc gccgtatatt cctgcagcgt
      961 gaacagcgcg aactggtcgg tcaggttgcg cgggacaaag gcttcggcgc cgaccgcgtt
     1021 gaaatcgcgg tggctgtact gcacgccgct cgcgccgcgc cagccgccgc ggtcgttctg
     1081 cgccagctcc agccggcctt cgacgccctg gttggtgaag atcgtgccga cctcgtcgcc
     1141 ttcgaactcg gtgtgcttgt aatcggcaaa gcccgcgcga atgcgcagct tgtcgaagaa
     1201 tccgtcgccg agctcgacct cgccgcgcag gtcggcgcgc cattgcttca tgccgatcgt
     1261 gacggggcct tcgccgtgat cgtggccgcc ttcctcttcc tcgccgccat gatcgtgcgc
     1321 ggtgttcggg cgcgcgggca cgccgtaatt ggtgtcgaaa tagccgaccg aaatgccgag
     1381 ctggccgccg tcgttgatca gcgagatgcc gccgcccgcg gtccaggtct cgctctgggt
     1441 gttgtcgatc ttgccgcgct tgcccgcctg ttcggtcagt tcggccgcct cgtcgagatg
     1501 gccttcttcg acttcatgct cggcaaggtg gagcaggtcg ccgcgaatat cgggggcata
     1561 gacgaagccg cccgagcgcg tgtcgccggt cttgcgccag ctgccgtcga gatgcgcgac
     1621 gatctgcggg ctcagcgcga cgtcgatcga gctgccgatg ttgcggtcgt tcgccgcggt
     1681 cgcataaccg ccgatcgcat cgacatggac atggtcggcg gggactttgc gcgggatacg
     1741 gcggtcgaac atattcaccg cgccgccgat cgcctggctg ccgaacagca gcaccgcggg
     1801 gccgcgcagg atttcgatac gctcgaccgt cagcgggtcg atcgtgacgg cgtgatccgc
     1861 cgacgtgttc gacgcgtcga tcgatccgat gccgtcggtc aggatcttga cgcgctcgcc
     1921 cgaaaagccg cgcaggacgg ggcgcgacgc gcccggcgag aagctcgtcg ccgacacgcc
     1981 cggttggcgg gtcagcgtat cgccgatctg cccgcgaatg tcgcgcgcca gctcgtcgcc
     2041 ctcgagcacc gacacattgc cgagaatatc gaggctgcgg acatagggcg cggtgacgat
     2101 gatcggctcg ccggtatgca catcgtcatc gccgccggca ttggcgcggt cctgcgcgaa
     2161 ggcgggggcc gcgaggaaaa gggcaagcga aaaaccggcg gaacaaagca aacgcatcgg
     2221 gggagagtcc tgtgattatg gcgaccagat ggcgcgtcag taatgatata ctgtcacagt
     2281 ggcaaccccc gatgcgtgcc ggacctgtcg ttcgtcggcc ttgggcgccc gttggtcgac
     2341 aagggcgatc atcggcgcgg caagcgaagc tgtcttgcgg gggcgtgggc gcggccctag
     2401 atcatgggca tgactattgc tgcaaacagc ctcgacctga tcggcaacac tccgctggtg
     2461 ctgctgaaag ggccgagcga agcgaccggc tgcgaaatct ggggcaagtg cgaatatgcc
     2521 aaccccggcg gttcggtgaa ggaccgcgcc gcgctttata tcgtccgcga cgccgaagcg
     2581 aacggcagtc tgcgccccgg cggcacgatc gtcgagggga cggcgggcaa caccggcatc
     2641 ggactggcgc tcgtcgccaa cgcgctcggc tatcggacga tcatcgtcat gcccgacaat
     2701 cagagcgccg agaaaatggc gacgatccgc gcgctcggcg ccgagctggt gctcgttccg
     2761 cccgcgccct tcgccaaccc cgggcatttc gtccacacct cgcgccgcat tgccgaggaa
     2821 accgacaatg cgatctgggc caaccagttc gacaatatcg ccaatcgcaa ggcgcatatt
     2881 ttcggcacgg ccgaggaaat ctgggagcag atggacggcc gcattgacgg cttcacctgc
     2941 gccgcgggca ccggcggcac gatcgcgggc accgggctgg ggctgaaggc gcatgatccg
     3001 gacatcgtca tcgcgctcac cgatccgcac ggcgcgggcc tctataatta ttatcagtgc
     3061 ggcgaactga agcccgaagg gtcgagcgtc gccgaaggca tcgggcagaa ccgcatcacc
     3121 gccaatctcg aaggcgcgcc gatcgacacg cagttccgta tctcggacgc cgaagggctt
     3181 gcggtggtcc gccgcctgct cgacgaggag gggctgtgcc tcggcctctc gtcggggatc
     3241 aacgtcgccg gcgcgatggc gctcgcgcgg caactgggtc cgggcaaacg tatcgcaacc
     3301 atcctctgcg acagcgggtt tcgctatctc tcgacgctct acaaccccga atggctggcg
     3361 agcaaaggtc tggaggcggc ggcgtgagcg ggatcaggat cgacaccagt ccggcgccgc
     3421 aacccgcgcc gcccccgccg cccgtcgcac cggtgaacga cacgatgcgc cgcctgcaga
     3481 tcggcattgc gggggtgctc actgtgctgc tgctcgtcgg catggcgggg ctgatcggcg
     3541 aacgtgcgc
//
LOCUS       DATMSU010000150         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_182872,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000150 DATMSU010000000
VERSION     DATMSU010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182872"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1374)
                     /gene="purT"
                     /locus_tag="VN034_04020"
     CDS             complement(196..1374)
                     /gene="purT"
                     /locus_tag="VN034_04020"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HWV59795.1"
                     /translation="MTPTAKILLLGSGELGREFVISAKRLGCHVVACDSYAAAPAMQV
                     ADEYEVFSMLDGDALRAAIEKHRPDHIVPEVEAIRTEILAEVEAKGFHVVPSARAAQL
                     TMNRDAIRDLAASELKLTTSTFEYAETREELRAAAERIGFPLVVKPVMSSSGKGQSTV
                     KDAAGVDAAWDYAAAGMRGDRLRVIAEAFIDFDYEITLLTVRHKDGVAFCPPIGHRQE
                     RGDYRESWQPAAMSDAALASAQDMATKVVDALGGHGIFGVEFFVKGDAVIFSELSPRP
                     HDTGMVTLISQPLSEFDLHARAILGLPIPAIAVADASASAVLLADRDAADFAITGLAD
                     ALMPPDAKTSVDVRIFGKPVTRPWRRMGVALAKVAGGTTDDARAAAVAAADKLAIDYR
                     "
     gene            1500..1931
                     /locus_tag="VN034_04025"
     CDS             1500..1931
                     /locus_tag="VN034_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV59796.1"
                     /translation="MHAIAELERTLPTEALFDRYHVGWETKDADLIASLHSADTIFQL
                     HDGSPVVEGREALRAHCRHLFAAYDFSMMMGKRIFGRDYWTFDWTMVLTLAMPGGTAF
                     VAHVGMLDVIMVNAEGEVVSKHVYPDIAQMESAFARAGIAR"
BASE COUNT          351 a          681 c          703 g          351 t
ORIGIN      
        1 gccgcctcct gcgcctgcgc ggcgggaccg gcgatcggga tcgcgctcgc aaggcacagc
       61 gcggcgacgc ggccgaagaa tttgggcatg aaacgctcct gtcgaacaac tatgcccgcc
      121 cgtgccgcat cgcgcgacag cacgccaaat gcgatgttgt ccggccgtgc aacggacacg
      181 gcgagccgac gtttgctagc ggtagtcgat cgccagttta tctgccgccg cgacggcagc
      241 ggcgcgcgcg tcgtcggtgg tgccgccagc gaccttggcc aatgcgaccc ccatgcggcg
      301 ccagggccgc gtcaccggtt tgccgaagat gcggacatcg accgaggttt tagcatcggg
      361 cggcatgagc gcgtcggcaa ggccggtaat cgcgaaatcg gcggcatcgc ggtcggcaag
      421 gagcacggcg ctggcgctcg cgtcggccac cgcgatcgcg gggatcggca ggccgaggat
      481 cgcgcgggcg tggaggtcga attcggacag cggctgcgag atcagcgtca ccattcccgt
      541 gtcatgcggg cgcgggctta gttcggagaa gatcaccgcg tcgcccttca cgaaaaattc
      601 gaccccgaaa atgccgtggc cgccgagcgc atcgacgacc ttggtcgcca tatcctgcgc
      661 actcgccagc gccgcgtccg acatcgccgc gggctgccag ctttcgcgat agtcgccgcg
      721 ttcctgccgg tggccgatcg gcgggcagaa ggcgacgccg tccttgtgcc gcacggtgag
      781 caaggtgatt tcgtagtcga aatcgatgaa cgcctcggcg atcacgcgca gccggtcgcc
      841 gcgcatcccg gcggcggcat agtcccacgc cgcatcgacc ccagcggcat ccttcaccgt
      901 gctctgtccc ttgcccgacg aggacatcac gggtttgacg accagcggga agccgatgcg
      961 ttccgccgcg gcgcgcaatt cctcgcgcgt ttcggcatat tcgaaggtcg aggtggtgag
     1021 cttaagctcg ctcgccgcaa gatcgcggat cgcgtcgcgg ttcatcgtca gctgcgccgc
     1081 gcgcgccgag ggaacgacgt gaaagccttt ggcctcgacc tcggcgagaa tctcggtgcg
     1141 aatcgcctcg acctcgggga cgatatggtc ggggcgatgc ttttcgatcg cggcgcgcag
     1201 cgcgtcgccg tcgagcatcg aaaaaacctc atattcgtcg gcgacctgca tcgccggggc
     1261 ggcggcgtag ctgtcgcacg cgacgacatg gcagccgagg cgttttgcgg aaatcacgaa
     1321 ttcgcggccg agctcgcccg agccgaggag gagaatcttt gcggtggggg tcatgggcgc
     1381 agctccgaat ctgaagcgcc tccacagccg atattgcgcc gccgcgcaat gcacgtttgt
     1441 tcaacgcgtc gggccggccc gggattagaa tggatcgcga cagacatagg agaaagacga
     1501 tgcacgcgat tgccgagctg gaacgcacgc tgccgaccga ggcgctgttc gaccgctatc
     1561 atgtgggctg ggagacaaag gacgccgacc tgatcgcgtc gctgcactcc gccgacacca
     1621 ttttccagct ccacgacgga tcgcccgtgg tcgagggacg cgaggcgctg cgtgcgcatt
     1681 gccgacatct tttcgcagcc tatgattttt cgatgatgat gggcaaaagg atcttcggcc
     1741 gcgactattg gaccttcgac tggacgatgg ttctgacgct cgcgatgccg ggcggcaccg
     1801 cctttgtcgc ccacgtcggc atgctcgacg tgatcatggt caatgccgaa ggcgaggtcg
     1861 tgagcaagca tgtctatccc gatatcgcgc agatggagtc ggccttcgcg cgggccggaa
     1921 tcgcgcgcta gatggcgccg ccaccgccct tgcgcgccga ccacggcggg tgaatagtcg
     1981 ccgccatgcc tgacccggtc gcgcctttcc tcaccagctg ctttccggtg gggacgcgcg
     2041 cgctcgcgcc gcacgccaat ctggtgacgg cggtgctcgc gggccg
//
LOCUS       DATMSU010000151         1719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_107252,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000151 DATMSU010000000
VERSION     DATMSU010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107252"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..>1719
                     /locus_tag="VN034_04030"
     CDS             144..>1719
                     /locus_tag="VN034_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWV59797.1"
                     /translation="MKLRTVTLALAALLSSTAAHAKGFLKVAGTEIVDGSGKPVILRG
                     MGLGGWMLQEGYMLKLGEVGQQHRIRAKLVELVGEERTAEFYRAWLDNHTTKADIDQM
                     AKWGFNSVRLPIHFDQLTLPADKEPNAGQDTWHEEGFRRIDRLLEWSKVNRIYLILDL
                     HAAPGGQGNDLAISDRDPAKPSLWQSAENQRKTVMLWTKLAARYKDEPWIGAYDLINE
                     PNWSFATPGKGNGCDETENKAVWALQQRITKAIRDVDPKHIVIIEGNCWGNNYKGLPP
                     AWDANMVLSFHKYWNRNDEASIAEIKALRASYNRPIWLGESGENSNGWYRDAIALVES
                     AGIGWNFWPLKKIGFNQPLEIVAGDGWMKIVAWATGKGPKPGADEAFAAMMQLAENSR
                     FDKNIPKPDVIDAMFRQPHDPAYRAFAPRALATEPLTIAAADYDLGPPGTAYHDTVDA
                     NYHVATGGERTPWNNGTTYRNDGVDIARETDGTPYVSDFVTGEFMRYSADVARAGRWT
                     VSARVRSAKGGRIGIDE"
BASE COUNT          361 a          549 c          545 g          264 t
ORIGIN      
        1 gaaggccggg atctcgacct ctcatcatca cgcaccggcg agatcccggc cttcgccggg
       61 atgacggagg cgacaaaatc tcgaccttgc tcttgccgca gagcttgata acgttcccat
      121 gattaaaaga tggggatgaa gtcatgaaac tccgaacggt cacgctcgct ctcgccgcgc
      181 tgctttcgtc gactgccgcg cacgccaaag gctttctgaa ggtcgcaggg accgagatcg
      241 tcgacggatc gggcaaaccg gtaatcctgc gcggcatggg gctcggcggc tggatgttgc
      301 aggaaggcta tatgctgaag ctcggcgagg tcgggcagca gcacaggatc cgcgccaagc
      361 tcgtcgaact ggtcggcgag gagcgcaccg cggaattcta tcgcgcctgg ctcgacaatc
      421 acacgaccaa ggccgacatc gaccagatgg cgaagtgggg tttcaactcg gtgcgcctgc
      481 cgatccattt cgaccagctc accctgcccg ccgacaagga acccaacgcc ggtcaggata
      541 cgtggcacga ggaaggtttc cggcggatcg atcgcctgct cgaatggagc aaggtgaacc
      601 gcatttacct gatcctcgat ctccacgccg cgccgggcgg ccagggcaat gatctcgcca
      661 tttccgaccg cgatccggcg aaaccgtcgc tgtggcaaag cgcggagaat cagcgcaaga
      721 ccgtgatgct gtggaccaag ctcgcggcgc gctacaagga cgagccgtgg atcggcgcct
      781 atgacctgat caacgagccc aactggagct tcgcgacccc cggcaagggc aatggttgcg
      841 acgagaccga gaataaggcg gtgtgggcgc tgcaacagcg catcaccaag gcgatccgcg
      901 acgtcgaccc aaagcatatc gtgatcatcg agggcaattg ctggggcaat aattacaagg
      961 gcctgccgcc cgcgtgggac gccaatatgg tgttgagctt ccacaaatat tggaaccgca
     1021 acgacgaggc gagcatcgcc gagatcaagg cgctgcgggc cagctataac cgcccgatct
     1081 ggctcggcga gtcgggcgag aacagcaatg gctggtaccg cgatgcgatc gcgctggtgg
     1141 agagcgccgg tatcggctgg aatttctggc cattgaagaa gatcggcttc aaccagccgc
     1201 tcgagatcgt cgccggcgac ggctggatga agatcgtcgc gtgggccacc ggcaagggcc
     1261 caaagccggg cgccgacgag gccttcgcgg cgatgatgca gctcgccgaa aacagccgct
     1321 tcgacaagaa tatccccaag cccgatgtca tcgacgcgat gttccgccag ccgcacgacc
     1381 cggcctatcg cgccttcgca ccgcgcgcct tggcgacgga accgctcacc atcgccgccg
     1441 ccgactacga cctcggcccg ccgggcacag cctatcatga cacggtcgat gccaattatc
     1501 acgtcgcgac cgggggcgag cggacgccgt ggaacaacgg cacgacctat cgcaacgacg
     1561 gcgtcgacat cgcgcgcgaa accgatggga cgccctatgt cagcgacttc gtcaccggcg
     1621 agtttatgcg ttacagcgcc gacgtcgccc gcgcggggcg ctggaccgtc agcgcgcgcg
     1681 tgcgctcggc caagggcggc cgcatcggca tcgacgaac
//
LOCUS       DATMSU010000152         4563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_116627,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000152 DATMSU010000000
VERSION     DATMSU010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116627"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1239)
                     /locus_tag="VN034_04035"
     CDS             complement(64..1239)
                     /locus_tag="VN034_04035"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HWV59798.1"
                     /translation="MPPEAPVCDAIEGYSAGFDGRRTFALRPADLEVIKAALPNDLAI
                     GAAYRDLIARADKALTAKPASVIDKRSIPMSGDRHDYVSLARYWWPNPANPKGAYVRR
                     DGDTDPEIESGRFDRAALARMVRDADTLALAHYYSGERKYAEGAARVIRAWFLDPATA
                     MNPHMNFAQAVPGVSNGRAEGVLDGAGFVAVIDAAGLIAPSGALTPTETSALEGWFSR
                     YLDWMLTSPNGRAEGKASNNHGLWYDAQVARFALFARRPDVARRIAAAFPKTRLARQF
                     HPSGALPKELTRTRSFHYSLYALDAAYTVADSAACLGIDLYRADEKGRSLRKATDYVA
                     AWRGRASDWPYKEQGWPAAMLDRLLVRADDAWGPGAYPRTVGSDLLLRHRATPEPPQ"
     gene            complement(1317..4238)
                     /locus_tag="VN034_04040"
     CDS             complement(1317..4238)
                     /locus_tag="VN034_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59799.1"
                     /translation="MKSSSVLPTRAALLASAALFAAMPVWAQEAPAEIEGEAAAAVDG
                     GDEILVTGTRATQRSSIEFKRAADVVVDGLVSEEIGATPDNSVGDTLERIVGVSADRF
                     KGNANELSVRGLGPTLSFSTFNGREVSTAGPDRSVAFQQFPSELVNGVLVYKTQRADF
                     LEGGVGGVIELRSMKPLDYGKRRIQFEVRGDFQPQDDDVYQHDGLGYRANVSYTDQYE
                     TGLGDIGVSIGYQRQDTTAPEDYYNGNSTFQLCNTAADNPWQLTGNAATQAEAGANIN
                     CTFAEGPRTANGVTVGETIGDYYFPTSSRSFRTQKTSEIRDGLIAAIQWRPSGEFEIA
                     IDGQYSKRRSLEDRNVLGIPEGLRGIEPLVIGDGSNGYSPGALISYRGNSNIENQLET
                     RQRNETYKGGGLNLIWTPDRWTVSFDGSYSDSHRTETQKATRMRSNRRVGYTLSYVDD
                     NVVPVVEFEDFDITDHDLFLTTAANSVYARNRFVTDRRDRIWAGRLDIERELDGFLTS
                     VKVGGRYSDHHRTNDNARNNDLNTLAAIDGTTPAELIAQANQNCRVPFTTTSYMQGMG
                     TNVTRWATFDNDCLFRTFTGGDDALPYPTDGRDPSDIDVTERIYALYGLANFESQAGS
                     VPFSGNIGLRWVKTDITSIGYRQPYVITIDTAGDSYSVDPDPNGELLTNRAKGSYNYF
                     LPSANIAFDLSQEVKLRLAAYRAIARSGIESFGAGIRLNPSAGTGLDNIIFDATTGNP
                     NLKPLRAWNLDASLELYASQDTLLSIAGYYKWAKGTVISAEEPIPTDVTITTIRDGGA
                     PVTETVTIAPVAPTNDGETRHLYGIEATASHAFTWLPDPLDGLGIQGSVNRAFANFEY
                     PDTSPIADYVDPANLIGLSKWTASGSVWFEKWGLSLRANLRYRSGYYKPNSGTNREIR
                     GATYINLSAQYNLTDNVQLKLQALNVTGTNDIMYKGGYDSIAEVSRSGTQYFFGFRVR
                     L"
BASE COUNT          737 a         1371 c         1596 g          859 t
ORIGIN      
        1 gcgggtgcgc ccatgcggcg ggcgcggcga gcagcgcggt gaggagcagg gcgcggcggt
       61 cgatcattgg gggggctcgg gggtggcgcg atggcgcagc aagagatcgc tcccaaccgt
      121 gcgcggatag gcgcccggac cccaggcatc gtcggcgcgc acgagcagcc ggtccagcat
      181 cgcggccggc cagccctgtt ccttgtacgg ccagtcggat gcgcggccgc gccaggcggc
      241 gacatagtcg gtggccttgc gcagcgagcg gcctttttca tcagcgcgat agaggtcgat
      301 gccaaggcac gccgcgctgt cggcgaccgt ataggcggca tcgagcgcat agagcgaata
      361 atggaagctg cgcgtccgcg tcagttcctt cggcagcgcg cccgagggat ggaattgccg
      421 cgcgagacgc gttttgggaa aggcggcggc gatccggcgc gcgacatcgg ggcgccgcgc
      481 gaacagcgcg aagcgcgcga cctgcgcgtc gtaccagagg ccgtgattgt tcgaggcctt
      541 gccctccgcc cttccgttcg ggctcgtcag catccagtcg agatagcgcg aaaaccagcc
      601 ttcgagcgcc gaggtttcgg tcggggtcag cgcgcccgac ggtgcgatca gcccggcggc
      661 gtcgatcacc gcgacaaagc ccgcgccgtc gagcacgcct tcggcgcggc cgttcgacac
      721 gccggggacg gcctgcgcga agttcatgtg cgggttcatc gccgtcgcgg ggtcgaggaa
      781 ccaggcgcgg atgacgcgcg cggcgccttc ggcatatttt cgttcgccgc tgtaataatg
      841 cgcgagcgcg agcgtatcgg catcgcgcac catgcgcgcg agcgcggcgc ggtcgaagcg
      901 accgctttcg atttcgggat cggtgtcgcc gtcgcggcgg acataggcgc ccttcgggtt
      961 ggcggggttc ggccaccaat agcgcgcgag gctgacatag tcgtggcggt cgcccgacat
     1021 agggatgctg cgcttgtcga tcaccgacgc gggtttcgcc gtgagcgcct tgtcggcgcg
     1081 cgcgatcagg tcgcgatagg cggcgccgat cgccagatcg ttcggcagcg ctgccttgat
     1141 cacctcgaga tcggcggggc gcagcgcgaa ggtgcggcgg ccgtcgaaac cggccgaata
     1201 gccttcgatt gcgtcgcaaa cgggagcttc cggcggcaag gaaaaagccg ccgccgcgga
     1261 cggagtcgcg gcggcggcca ggatagccca agggagtcgg gctaaagtcg atggcttcac
     1321 aggcggaccc ggaagccgaa gaaatattgc gtgccgctgc gcgacacttc ggcgatgctg
     1381 tcgtatccgc ccttgtacat gatgtcgttg gtgccggtga cgttgagcgc ctgcagcttg
     1441 agctggacat tgtcggtgag attatattgc gccgacaggt tgatataggt cgcgccgcga
     1501 atctcgcggt tggtgccgct gttcggtttg taatagcccg agcggtagcg aaggttggcg
     1561 cgcagcgaga ggccccattt ttcgaaccac accgatccgc tcgcggtcca tttcgacagc
     1621 ccgatcaggt tcgccggatc gacatagtcg gcgatcggcg aggtgtcggg atattcgaaa
     1681 ttggcgaagg cgcggttgac cgatccctgg atgccgagcc cgtcgagcgg gtcgggaagc
     1741 caggtgaagg cgtggctcgc ggtcgcctcg atcccgtaga gatgccgcgt ttcgccatcg
     1801 ttggtcggcg ccaccggcgc gatggtgacg gtctcggtca ccggcgcgcc gccgtcgcgg
     1861 atcgtcgtga tcgtgacgtc ggtggggatc ggctcctcgg cgctgatcac tgtgcccttc
     1921 gcccatttat aatagcccgc gatcgagagc aaagtgtcct gtgacgcgta gagctcaagg
     1981 ctcgcgtcga ggttccacgc gcgtagcggc ttgaggttcg ggttgcccgt cgtcgcgtcg
     2041 aagatgatat tgtcgagccc ggtccccgcc gaggggttga gccggatgcc cgcgccgaag
     2101 ctttcgattc ccgaccgcgc gatcgcgcga taggcggcga ggcgcagctt gacctcttgc
     2161 gacaggtcga aggcgatatt cgcgctcggc aggaaataat tatagctgcc cttggcgcgg
     2221 ttggtcagca gctctccgtt cggatcgggg tcgacgctat agctgtcgcc ggcggtgtcg
     2281 atcgtaatca catagggctg acgatagccg atcgaggtga tgtcggtttt gacccagcgc
     2341 agcccgatat tgccgctgaa cggcacgctg cccgcctgcg attcgaaatt ggcgaggccg
     2401 tagagcgcat agatgcgttc ggtgacgtcg atatcgctcg ggtcgcggcc gtcggtcgga
     2461 tagggcagcg cgtcgtcgcc gccggtgaag gtgcggaaca ggcagtcgtt gtcgaaggtc
     2521 gcccagcgtg tgacgttggt gcccatgccc tgcatgtagc tggtggttgt aaagggcacg
     2581 cggcagttct ggttcgcctg cgcgatcagc tcggcggggg tcgtgccgtc gattgcggcc
     2641 agcgtgttga gatcgttgtt gcgcgcattg tcgttggtgc ggtgatgatc cgaatagcgg
     2701 ccgccgacct tgaccgaggt gaggaagccg tcgagctcgc gctcgatatc gagccgcccg
     2761 gcccagatcc ggtcgcggcg gtcggtgacg aagcggttgc gtgcatagac cgaattggcg
     2821 gcggtggtca ggaacaggtc gtggtcggtg atgtcgaaat cctcgaattc gaccaccggc
     2881 acgacgttat cgtcgacata ggagagcgtg taaccgacgc ggcggttcga gcgcatgcgc
     2941 gtcgccttct gcgtttcggt gcggtggctg tccgaatagg atccgtcgaa gctcaccgtc
     3001 cagcgatcgg gggtccagat cagattgagg ccgccgccct tgtaagtttc attgcgctgg
     3061 cgcgtttcga gctggttctc gatattcgaa ttgccgcgat agctgatcag cgcgccgggc
     3121 gaatagccgt tgctgccgtc gccgatgacc agcggttcga tgccgcgcag cccttcggga
     3181 atgcccagca cattgcggtc ctcgagactg cggcgcttcg aatattggcc gtcgatcgcg
     3241 atctcgaact cgcccgacgg gcgccactgg atcgcggcga tcagcccgtc gcgaatctcc
     3301 gacgtctttt gggtgcggaa gctgcgcgac gaggtcggga aataatagtc gccgatcgtc
     3361 tcgccgacgg tcacgccatt ggcggtgcgc ggcccctcgg caaaggtgca gttgatgttg
     3421 gcgcccgcct cggcctgcgt cgcggcattt ccggtcagtt gccacggatt gtcggccgcg
     3481 gtgttgcaga gctgaaaggt cgaattgccg ttgtaataat cctcgggcgc ggtcgtgtcc
     3541 tggcgctggt aaccaatcga cacgccgata tcgccgagcc cggtttcata ttggtcggtg
     3601 taggagacat tggcgcgata gccgagcccg tcgtgctgat agacgtcgtc gtcctgcggt
     3661 tggaaatcgc cgcgcacctc gaactggatg cgccgcttgc cgtaatcgag cggcttcatc
     3721 gagcgaagct cgatcacccc gccgacgccg ccttcgagga agtcggcgcg ctgggtcttg
     3781 tagacgagga cgccgttgac cagttcggac gggaattgct gaaaggcaac cgagcggtcg
     3841 ggtcccgcgg tcgacacctc gcggccgttg aaggtcgaaa agctcagggt cgggccgagg
     3901 ccgcgcaccg acagttcgtt ggcattgccc ttgaaacggt cggccgagac gccgacgatg
     3961 cgttcgagcg tgtcgccgac cgaattgtcg ggggtcgcgc cgatctcttc gctgacgagg
     4021 ccgtcgacga cgacgtcggc ggcgcgcttg aactcgatcg aactgcgctg cgtcgcgcgc
     4081 gtgccggtga cgagaatctc gtcgccgcca tcgaccgccg ccgccgcctc gccttcgatt
     4141 tcggcgggcg cttcctgcgc ccaaacgggc atggcggcga acagcgccgc ggacgccagc
     4201 aaggcggcgc gggtcggcag gaccgatgat gacttcattt ccctctcctt tttatgccgc
     4261 ccctggattt gcggcgcgga cccggttgat accggtgtca atttcagttg ttatacaact
     4321 tctaagatgt attacaactt taaaaggaga agtcaatcat ccgcattgca ggacggtatc
     4381 aaccgcaaac gacccaattt agcgagggtt tgcgcgcttt ttcgccaagt catacatcgc
     4441 ggttcctgcg agcagatagg cgccgctgcc gtagaattgc gtttccttcg ccgagacgct
     4501 gtcggggcgg tcgccgacct gctggaccca gccgagcatc ccgtcgggct ggaccgcgcg
     4561 gcc
//
LOCUS       DATMSU010000153         4178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_154407,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000153 DATMSU010000000
VERSION     DATMSU010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154407"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..822)
                     /locus_tag="VN034_04045"
     CDS             complement(70..822)
                     /locus_tag="VN034_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59800.1"
                     /translation="MTLQGKTALVTGGGRDIGRSVSIALARAGVRVAINYNSGRDAAE
                     DTLKTIKDAGGDAFLVQADVTDSNAVRAMLDEVGVAFSGRLDILVNLAGGMVARKTLS
                     EMDEDFFDHVMTLNLKSAFLVLQASQPFLGEGSAVVNVSSLAGRDGGGPGASVYATAK
                     GALMTYTRAMAKELGPQGIRVNAVCPGLIGTSFHDIFSKPEGRAATAGNTPLRREGHP
                     DEVADTIVYLASPAASFLAGVCLDINGGLAFS"
     gene            complement(836..2119)
                     /locus_tag="VN034_04050"
     CDS             complement(836..2119)
                     /locus_tag="VN034_04050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59801.1"
                     /translation="MSNIKGRFRWGVISLIALATVINYIDRNALAVMWPEVSKEIGAT
                     KEDYALLVTIFMIFYAFGQSLFGRIFDAIGTRMGFTLSIIVWSLSIAAHALVRSMPLL
                     VFLRATLGVSEAGNWPGAAKANALWFPSRERALAQGIFNAGASLGGIISAPLIALLFV
                     QFGWHGTFLLIGVLGFIWLVPWLWFYKADPDKHPGLSEEERTYILTGRTEAGRDDGRR
                     VPLGELLRYRQSWGVILSRFFLDPVWWLFVSWLPIYLAETFGFDVKQIGLFAWVPFVG
                     AMLGSLFGGWFAGRIITGGGSVHKARTLSIAIGCAIMLPALLLTIGASGPLTAVLLIA
                     CILFGFQMAIGNIQTLPSDYFGGGAVGSLAGISGTAAVAGTLITTWLVPVLTKTSYAP
                     IFALSAAIVPISFLCFWLIGGRVEPVVTKTTHNQE"
     gene            complement(2116..>4178)
                     /locus_tag="VN034_04055"
     CDS             complement(2116..>4178)
                     /locus_tag="VN034_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWV59802.1"
                     /translation="LFAAEVDRARRDVDASIKAGINVPVPKDPGGGFTHEQHKRNYRI
                     IFEGGQLFRLTGEARYRDHVRDLLLAYADLYPGLGPHPAKANQNAGRLFWQSLNDSVF
                     LVNAVQGYAQIRDALPAADRKRIDDQVIRPMARFLSDESPQVINRIHNHATWAAAGVG
                     LSGYLLGDRDLVDKALLGLDKSGKAGFLRQLDLLFSPDGYYAEGPYYQRYALQPFVVF
                     AAAIAANDPDRKIFEYRDGIVLKAIRTAIDLTHDGYFLPINDAMPDKSLRTEELYHAV
                     AIAYGATKDPAFLSIADWQGRTVLTPAGQAMAADLAAGKAKPWPFGSRLLSDGPDGKG
                     GALVLLRTNADPAGPLLVAKNTVQGMGHGHFDRLGWLYYDETGAVVTDYGAARFLNVE
                     AKRGGRYLPENDSWASATIAHNTLVVDERSHFTGDWKAGEKSGTRQLAASLEGPTRYT
                     IAEIDSAYKDVAIRRALLLIEVDGLANPLTLDILHGTGSGRHVYDLPLHFSGHIIDSD
                     IAFDRNLAERPVLGKANGYQHLWVDGTGTKTGKARLTWMQGSRFYSYHMLPPAGASFI
                     LAESGANDPEFNLRREPALIQRVDGAANATFVSLLEPHGAYDAAAETVVASSARVAAL
                     EHRKEKGADLVLITLIDGRRLAIAVADDVAADAKHSLTVDGRTLAWTGPVGRLDLAKT
                     GAKR"
BASE COUNT          703 a         1310 c         1481 g          684 t
ORIGIN      
        1 gttcgcgcgg cgcggcctgc gctccgctcg cgagcagagc acaggcggcg atggcggcca
       61 gccggcgcat caggagaagg ccaatccgcc gttgatgtcg aggcagacgc cggcgaggaa
      121 gctcgcggcg ggcgaggcga gatagacgat cgtgtcggcg acctcgtcgg gatggccttc
      181 gcggcggagc ggcgtgttac ccgcggtcgc agcgcgccct tcgggcttcg agaagatgtc
      241 gtggaagctg gtgccgatca ggcccgggca gacggcgttg acgcggatgc cctgcggccc
      301 gagttccttc gccatcgcgc gggtataggt catcagcgcg cccttcgcgg tcgcatagac
      361 cgacgcgccc gggccgccgc cgtcgcggcc agcgagcgac gagacgttca ccaccgccga
      421 tccttcgcca aggaagggct gcgatgcctg gaggacgagg aaggccgatt tgaggttgag
      481 cgtcatgaca tggtcgaaga agtcctcgtc catctcgctg agcgtcttgc gcgcgaccat
      541 gccgcccgcg aggttgacga ggatgtcgag ccggccgctg aacgcgacgc cgacctcgtc
      601 gagcatcgcc ctgacggcat tgctgtcggt gacgtcggcc tgaaccagaa aggcgtcgcc
      661 gcccgcgtcc ttgatcgtct tcagcgtgtc ctcggcggca tcgcggccgc tgttgtaatt
      721 gatcgcgacg cggacgccgg cgcgcgcgag cgcgatcgag accgagcggc cgatgtcgcg
      781 accgccgccg gtgacgagcg cggttttgcc ctggagagtc atgtgatgct attccttatt
      841 cttgattgtg ggtggttttg gtgacgaccg gttcgacgcg tccgccgatc agccagaaac
      901 agaggaacga aatcgggacg atcgcggcgc tgagcgcgaa gatcggcgcg tagctcgtct
      961 tggtgagtac cgggacgagc caggtggtga tcagcgtgcc cgcgaccgcc gcggtgccgc
     1021 tgatcccggc gaggctgccg accgcgccgc cgccgaaata gtcgctcggc agcgtctgga
     1081 tattgccgat cgccatctgg aaaccgaaga ggatgcacgc gatgagcagc accgcggtca
     1141 agggaccact cgcgccgatc gtcagtagca aagcgggcag catgatcgcg cagccgatcg
     1201 cgatgctcag cgtgcgcgcc ttgtggacgc tgccgccgcc ggtgatgatc cgccccgcga
     1261 accagccgcc gaacaggctg ccgagcatcg cgccgacgaa gggcacccac gcgaaaagcc
     1321 cgatctgctt gacgtcgaag ccgaaggttt cggcgagata gatcggcagc cacgatacga
     1381 agagccacca gacggggtcg aggaagaagc gcgacaggat gacgccccag ctctgccgat
     1441 agcgcagcag ttcgcccaag ggcacgcggc ggccgtcgtc gcgcccggcc tcggtgcggc
     1501 cggtcaggat ataggtgcgt tcttcctcgc tgagccccgg atgcttgtcg gggtcggcct
     1561 tgtagaacca gagccagggc acgagccaga tgaagccgag cacaccgatg aggaggaagg
     1621 tgccgtgcca gccgaactgg acgaagagca aggcgatcag cggcgccgaa atgatgccgc
     1681 caagcgaggc gcccgcgttg aagatgccct gcgccagcgc gcgttcgcgc gaggggaacc
     1741 agagcgcgtt cgccttggcc gcaccgggcc agttgcccgc ctcgctgacc ccgagcgtcg
     1801 cgcgcaggaa gacgagcagc ggcatcgagc gcaccagcgc gtgcgccgcg atcgacagcg
     1861 accagacgat aatcgagagg gtgaagccca tccgcgtccc gatcgcgtcg aagatgcgcc
     1921 cgaacagcga ctggccgaag gcgtagaaga tcatgaagat cgtcaccagc agcgcgtaat
     1981 cttccttggt ggcgccgatt tccttcgaca cctcgggcca catcaccgcg agcgcgttgc
     2041 ggtcgatata gttgatcacg gtggcgagcg cgatcagcga gatcacgccc cagcggaagc
     2101 ggcctttgat attgctcatc tcttcgctcc ggtcttggcg aggtcgagac ggccgacggg
     2161 gccggtccat gcgagggtgc ggccgtcgac ggtgaggctg tgcttcgcat cggcggcgac
     2221 gtcgtcggcg accgcgatcg cgaggcggcg tccgtcgatg agggtgataa ggacgaggtc
     2281 ggcgcccttt tccttgcgat gctcgagcgc cgcgacgcgc gcgctgctcg cgaccaccgt
     2341 ctcggccgcg gcgtcatagg cgccgtgcgg ttcgagcagg ctgacgaagg tcgcgtttgc
     2401 ggcgccgtcg acgcgctgga tcagcgcggg ttcgcggcgc aggttgaact cgggatcgtt
     2461 cgcgccgctt tcggcgagga tgaagcttgc gcccgcgggc ggtagcatat ggtagctata
     2521 gaagcggctg ccctgcatcc acgtcagccg cgccttgccg gtcttggtcc ccgtcccgtc
     2581 gacccacaga tgctgatagc cgttcgcctt gccgagcacc ggccgttcgg caagattgcg
     2641 gtcgaacgcg atgtcgctgt cgatgatatg ccccgaaaaa tgcagcggca ggtcatagac
     2701 atgcctgccg ctgccagttc cgtggaggat atcgagcgtg aggggattgg cgaggccgtc
     2761 gacctcgatc agcagcagcg cgcggcggat cgcgacgtcc ttgtaagcgc tgtcgatttc
     2821 ggcgatcgtg tagcgggtcg ggccttcgag cgatgcggcg agctgacgcg tgccagattt
     2881 ttccccggct ttccaatcgc ctgtgaagtg gcttcgctca tcgacgacaa gcgtgttgtg
     2941 cgcgatcgtc gcgctcgccc agctgtcgtt ctcggggagg tagcgcccgc cgcgcttcgc
     3001 ctcgacattg aggaagcgcg cggcgccata gtcggtcacg accgcgccgg tttcgtcata
     3061 atagagccag ccgagccggt cgaaatggcc gtgccccatg ccctgcaccg tattcttggc
     3121 gacgagcagc ggacccgcgg gatcggcgtt ggtgcgcagc agcacgagcg ccccgccctt
     3181 gccgtcgggg ccgtcggaga ggagccgcga gccgaagggc cagggcttcg ctttgcccgc
     3241 cgcgaggtcg gcggccatcg cctgccccgc cggagtcagc acggtgcgcc cctgccagtc
     3301 ggcgatcgac aggaaggcgg gatccttggt ggcgccatag gcgatggcga cggcgtgata
     3361 gagttcctca gtgcgcaggc tcttgtcggg catcgcgtcg ttgatcggca ggaaatagcc
     3421 gtcgtgggtc agatcgatcg cggtgcgaat tgctttcagc acgatgccgt cccgatattc
     3481 gaagatcttg cggtcgggat cgttcgcggc gatcgcggcc gcgaagacga cgaaaggttg
     3541 cagcgcatag cgttgataat agggcccttc ggcataatag ccgtcgggcg agaagagcag
     3601 gtcgagctgg cggaggaagc ccgccttgcc gctcttgtcg agcccgagca acgccttgtc
     3661 gacgaggtcg cggtcgccga gcagatagcc cgacaggccg acgcccgccg ccgcccaggt
     3721 cgcgtggtta tgtatgcggt tgatgacctg gggcgactcg tccgacagga agcgcgccat
     3781 cggacggatc acttggtcgt cgatgcgctt gcggtcggcg gcgggcagcg catcgcggat
     3841 ctgggcatag ccctgcaccg cgttgacgag gaaaacgctg tcgttgaggc tctgccagaa
     3901 gaggcgcccc gcgttctggt tcgcctttgc cggatgcggc ccgagccccg gatagaggtc
     3961 ggcataggcg agcagcaggt cgcggacatg gtcgcgatag cgcgcctcgc cggtcaggcg
     4021 gaaaagctgg ccgccttcaa aaatgatgcg gtaattgcgc ttgtgctgtt cgtgggtgaa
     4081 gcccccgccg ggatccttgg gcacgggcac gttgatgccc gccttgatcg acgcatcgac
     4141 atcgcgccgc gcgcggtcga cctcggcggc gaagagcg
//
LOCUS       DATMSU010000154         4462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_154427,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000154 DATMSU010000000
VERSION     DATMSU010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154427"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="VN034_04060"
     CDS             <1..878
                     /locus_tag="VN034_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV59803.1"
                     /translation="ARASAASARSDLALAKLELDRASRLVDIEAVSQSEIDRLRATVN
                     AATAALAGADARIAARALDVEFTTVRAPLSGRISDRQIDAGNLVSAGDAGGTLLTTIN
                     ALDPIYFTFQGSEALFLKTKREGGQKGADVEVRLQDENDYRWKGRIDFTDNGLDPRSG
                     TIRARGRFDNPEMFLTPGMFGNMRLSTGQTARALLVPAAAVQTDQARKIVYVVGKDGM
                     VAAKPVELGPEVDGLRVIRSGLTPADRVVINGYQFARPGEKAVTKAGKIAADAPDKAP
                     AGPSEPVSAQATFAK"
     gene            1049..4240
                     /locus_tag="VN034_04065"
     CDS             1049..4240
                     /locus_tag="VN034_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWV59804.1"
                     /translation="MRLSRFFIDRPIFAAVLAVIITIVGAVAYIGLPVSQYPDIVPPT
                     VTVTATYPGASAETVAETVAAPIEQEINGVDNMLYMSSQSTGDGVVTITVTFKIGTDL
                     DAAQVLVQNRVAIATPRLPETVQRLGVVTRKTSPDFLLIVNLVSPDKSLDRAYISNYA
                     LTQLRDRLSRIDGVGDVRLFGSRDYAMRVWIDPGRAAALDLTAGEIVAALRRENVQVA
                     AGSLGQPPHANGSSFQLSVETQGRLTDPKDFANIVIRSDADGRQVRVSDVARVELGAS
                     DYNSNTYLSGDPTVILATFQRPGSNALAAAQAVEAEMAAASKSFPKGLEYRVIYNPTE
                     FIAQSIDAVMETLFEAVILVVIVILVFLQKWRAAIIPVLAIPVSLIGTFVVLAAFGYS
                     LNNLSLFGLVLAIGIVVDDAIVVVENVERNLEKGMSALEAARTSMDEVSGALVAIVLV
                     LCAVFVPTLFLTGLSGAFYQQFAVTISTATIISLVLSLTLSPALAAILLRPHAPAQAD
                     GWLMTRIHRAGERFNEGFERLSNGYARLTDRLVRAPKKMMVTYAGLIAATAGLFWATP
                     TGFIPAQDQGYFLVVAKLPSGASVERTDAVLKKVAGRVLPIEGVLGSVMLAGFDGPSQ
                     TLAPNSAAAYFPLKSFEERGKLGVNFEDIMNQARANTADITEAQIVIIPPPLIQGIGS
                     AGGYRMIVQDRGGNGYAALANETNNLIGKANETPGLANVFTFFDPTNPRVFADIDRAK
                     ANMLGVPPERVFEALQVYLGSAFVNDFNLLGRTYRVTAQADAPYRQTVADIANLRTRS
                     DSGAMVPIGSVANFEDRTGPYRVTRYNLFPAVEIDGDTAPGSSSGASLTTMEKLASES
                     LPAGYGTEWTGIAFQQKAAANTAGIVFALAVVFVFLVLAAQYESLMLPLAIIFIVPMC
                     LLAAMVGVNLRGMDNNVLTQIGLVVLIALAAKNAILIVEFAKQAEEEDGLSPIEAAVR
                     AARDRLRPILMTSFAFILGAVPLLIASGAGAELRQALGTAVFFGMLGVTAFGLIFTPT
                     FYVVARSLSHWSAERRARRGSDDHGTALPVPAE"
     gene            4252..>4462
                     /locus_tag="VN034_04070"
     CDS             4252..>4462
                     /locus_tag="VN034_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV59805.1"
                     /translation="MILRNILTAASALALAACAVGPNYTAPKSASAPVATGPLVSANS
                     PAVSLDPVEADWWRLYDDPVLDGLVA"
BASE COUNT          713 a         1515 c         1450 g          784 t
ORIGIN      
        1 aagcgcgcgc ctcagcggcg agcgcgcgca gcgacctcgc gctggcaaag ctcgaactcg
       61 accgcgcgag ccggctcgtc gatatcgaag ccgtgtcgca gagcgagatc gaccggctcc
      121 gggccacggt caatgcggcg acggcagcgc tcgccggtgc cgacgcgcgc atcgcggcgc
      181 gtgcgctcga cgtcgaattc accaccgtcc gcgcgccgct ttccggccgc atctcggatc
      241 gccagatcga cgcgggcaac ctcgtgtcgg cgggcgatgc cggcggcacc ttgctcacga
      301 cgatcaatgc gctcgacccg atctatttca ccttccaggg ttcggaagcc ttgttcctca
      361 agaccaagcg cgaaggcggc cagaagggcg ccgacgtcga ggtccgcttg caggacgaga
      421 atgattatcg ctggaagggc cggatcgact tcaccgacaa tggcctcgat ccgcgctcgg
      481 gcacgatccg cgcgcgcggc cgcttcgaca atcccgagat gttcctgacc cccggcatgt
      541 tcggcaacat gcgcctgtcg acgggccaga cggcgcgcgc gctgctcgtc cccgccgccg
      601 cggtgcagac cgaccaggcg cgcaagatcg tctatgtcgt cggcaaggac ggcatggtcg
      661 cggcgaagcc ggtcgagttg ggccccgaag tcgatggcct gcgcgtcatt cgttcggggc
      721 tgacgccggc cgaccgcgtc gtgatcaacg gctaccaatt cgcgcgtcct ggcgaaaagg
      781 cggtgaccaa ggccggcaag atcgccgccg acgcgcccga caaggcgccg gcgggtccga
      841 gcgagccggt gtcggcgcag gcgaccttcg cgaagtgaca taattagccc ctccccttca
      901 ggggaggggt tggggtgggg ggccgcgacc tcacgcaagg ccgatggtcc ccaccccgct
      961 gcgactaggg agcaagctcc caagtctcgc tgcccctccc ctgaagggga ggggctgagc
     1021 aaaaccttat ccagacaggg gctctcccat gcgcctatca cgcttcttca tcgaccggcc
     1081 gatcttcgcc gccgtgctcg ccgtcatcat caccatcgtc ggcgcggtcg cctatatcgg
     1141 gctgcccgtg tcgcaatatc ccgacatcgt cccaccgacg gtgaccgtga ccgcgaccta
     1201 tccgggtgcg tcggctgaaa ccgtcgccga gacggtcgcg gcaccgatcg agcaggaaat
     1261 caacggcgtc gacaatatgc tctatatgag cagccagtcg accggcgacg gcgtcgtcac
     1321 gatcaccgtg accttcaaga tcggcaccga ccttgatgcc gcgcaggtct tggtgcagaa
     1381 ccgcgtcgcg atcgcgaccc cgcgcctgcc cgaaacggtg cagcggctgg gcgtcgtgac
     1441 gcgcaagacc tcgcccgact tcctgttgat cgtgaacctg gtgtcgcccg acaagtcgct
     1501 cgatcgtgcg tacatctcca actatgcact gacccagctc cgcgatcgat tgagccgcat
     1561 cgacggcgtc ggcgacgtgc gcctgttcgg cagccgcgac tatgcgatgc gcgtgtggat
     1621 cgatcccggc cgcgctgctg cgctcgatct gaccgcgggc gagatcgtcg ccgcgctgcg
     1681 ccgcgaaaat gtgcaggtcg ccgcgggttc gctcggccag ccgccgcatg ccaatggcag
     1741 cagcttccag ctcagcgtcg aaacgcaggg ccgcctgacc gaccccaagg acttcgcgaa
     1801 tatcgtcatc cgcagcgatg ccgacgggcg gcaggtccgt gtgtcggacg tcgcgcgcgt
     1861 cgagcttggc gcgtcggact ataattcgaa cacctatctg tcgggcgatc cgacggtcat
     1921 tcttgcgacc ttccagcgcc cgggttcgaa cgcactcgcc gcggcgcagg cggtcgaagc
     1981 cgaaatggcg gcggcatcga aaagcttccc caagggcctc gaatatcgcg tcatctataa
     2041 ccccaccgaa ttcatcgccc agtcgatcga cgcggtgatg gagacgttgt tcgaagccgt
     2101 catcctcgtc gtcatcgtca tcctggtttt cctgcagaaa tggcgcgcgg cgatcatccc
     2161 ggtgctcgcg atcccggtgt cgctgatcgg caccttcgtg gtgctcgcag ccttcggtta
     2221 cagcctcaac aatttgtcgc tgttcgggct ggtgctcgcg atcggcatcg tcgtcgatga
     2281 cgcgatcgtc gtcgtcgaga atgtcgaacg caatctcgag aaaggcatga gcgcgctcga
     2341 agccgcgcgg acgtcgatgg acgaagtgtc gggcgcattg gtggccatcg tgctcgtgct
     2401 ctgcgcggtg ttcgtgccga ccttgttcct caccgggctg tcgggcgctt tctatcagca
     2461 gttcgcggtg acgatctcga ccgcgacgat catctcgctc gtgctctcgc tcacgctgtc
     2521 gcctgcgctg gcggcgatcc tgctgaggcc gcacgccccc gcgcaggccg acggatggct
     2581 gatgacgcgt atccaccgtg ccggcgaacg cttcaacgag ggcttcgagc ggctcagcaa
     2641 tggctatgcg cggctcaccg accggctggt gcgcgcgccg aagaagatga tggtcaccta
     2701 tgccgggttg atcgcagcga ccgcgggcct gttctgggcg acgccgaccg gcttcattcc
     2761 cgcgcaggat cagggctatt tcctcgtcgt cgcgaagctg ccctcgggcg cctcggtcga
     2821 gcgtaccgat gcggtgctga agaaggtcgc aggccgggtg ctgccgatcg agggtgtcct
     2881 tggctcggta atgctcgcgg gcttcgacgg cccgtcgcag acgctcgcgc cgaactcggc
     2941 ggccgcttac ttcccgctca agtcgttcga ggagcgcggg aagctcggcg tcaatttcga
     3001 ggacatcatg aatcaggcgc gtgcgaacac cgccgacatc accgaggcgc agatcgtgat
     3061 catcccgccg ccgctgatcc agggcatcgg ttcggcgggc ggttaccgga tgatcgtcca
     3121 ggatcgcggc gggaacggct atgccgcgct cgcgaacgag acgaacaatc tgatcggcaa
     3181 ggcgaacgag accccggggc tcgcgaacgt cttcaccttc ttcgatccga ccaacccgcg
     3241 cgttttcgcg gacatcgacc gggcgaaggc gaatatgctt ggcgtgccgc ccgagcgcgt
     3301 gttcgaggcg ttgcaggtct atctcggctc ggcgttcgtc aacgacttca acctgctcgg
     3361 ccgcacctac cgtgtgaccg cacaggccga cgcgccctat cgccagaccg tcgccgatat
     3421 cgcgaacctc cggacgcggt cggattcggg cgcgatggtg ccgatcgggt cggtcgcgaa
     3481 tttcgaggac cgcaccgggc cgtaccgcgt cacgcgctac aacctgttcc ccgcggtcga
     3541 gatcgacggc gacaccgcgc cgggttcgtc gtcgggcgcg tcgctgacga cgatggaaaa
     3601 gctcgcgtcg gagtcgcttc cggcgggcta tggcaccgaa tggacgggca tcgccttcca
     3661 gcagaaggcg gcggcgaaca ccgcggggat cgtcttcgcg ctcgcggtgg tcttcgtctt
     3721 cctcgtgctc gcggcgcaat atgaaagcct gatgctgccc ttggcgatca tcttcatcgt
     3781 cccgatgtgt ctgctcgcgg cgatggtcgg cgtgaatttg cgcggcatgg acaataatgt
     3841 tctgacgcag atcgggcttg tcgtgctcat cgcgcttgcg gcgaagaatg cgatcctgat
     3901 cgtcgaattc gccaagcagg ccgaggaaga agatgggctg tcgccgatcg aagccgcggt
     3961 gcgcgccgcg cgcgaccgtc tgcgcccgat cctgatgacc agcttcgcct tcatcctcgg
     4021 cgcggtgccg ttgctgatcg caagcggggc aggcgcggag ctgcggcagg cgctcggcac
     4081 cgcggtcttc ttcggcatgc tcggcgtgac ggcgttcggc ctcatcttca ccccgacctt
     4141 ctacgtcgtc gcacgctcgc tcagccactg gtcggccgag cggcgcgcgc ggcgcgggtc
     4201 ggacgaccac gggacggcgc tgccggttcc ggcggaataa ggaactagat catgatcctt
     4261 cgcaacatcc tcaccgccgc ctcggcgctg gcgctcgccg cctgtgccgt tggccccaac
     4321 tataccgcgc ccaaatcggc gtcggcgccc gtcgccaccg gtcccttggt ctcggcgaac
     4381 agccccgcgg tgtcgctcga tccggtcgaa gccgactggt ggcgcctcta tgacgatccc
     4441 gtcctcgacg ggctcgtcgc cg
//
LOCUS       DATMSU010000155         1852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_116664,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000155 DATMSU010000000
VERSION     DATMSU010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116664"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..390)
                     /locus_tag="VN034_04075"
     CDS             complement(82..390)
                     /locus_tag="VN034_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59806.1"
                     /translation="MSHPSNIVYCTGPGDPHALDGISVRHRTGDLDRLCPVCAGHGQW
                     NSQIDLVSHRSIRVPCPKCDGRGWIETGADMVPSHDIALSPGGQPRWVVRLDPSDDIE
                     "
     gene            complement(411..992)
                     /locus_tag="VN034_04080"
     CDS             complement(411..992)
                     /locus_tag="VN034_04080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWV59807.1"
                     /translation="MATITIGKPADRARLLHTLVLGFAADPVARWASPDAATYMSRRH
                     EFFGAFGGAAFEHGTAFVADDGAAVATWLPPGVEPDGEVMAAIMDEQIPAHRKAEMDA
                     LVEQMDHFHPKEPVWYLPLIAADPAHRGRGLGTALMEAAIAVIDADGRAAWLESSNPR
                     NIPLYRRFGFEITGEIRTITSPLLTPMLRPGRG"
     gene            complement(1033..1560)
                     /locus_tag="VN034_04085"
     CDS             complement(1033..1560)
                     /locus_tag="VN034_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HWV59808.1"
                     /translation="MRARNPLYVMAKPPPEVQAAIAALPRNDPGRGPDLLHVTLISLY
                     DLHHAPPGWLPATIAALDGFAAAPFPLRFDRIENRKAVTLRTRDPLAGARAFQKALVD
                     HLLREKAPIMDGTTPEPHITINYRGDRLGSQTFGALKLPPIEWTVREILLMESVVGKT
                     THIEHGRWTLTPPGD"
     gene            complement(1650..>1852)
                     /locus_tag="VN034_04090"
     CDS             complement(1650..>1852)
                     /locus_tag="VN034_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59809.1"
                     /translation="QRLKAAGIAPGGLTDAEFARQIGLEEARRAAAGTTLTVDFGDDG
                     KADDAAEGQEPPEGGTQEGYVA"
BASE COUNT          254 a          618 c          692 g          288 t
ORIGIN      
        1 tctcggcagc gccctgcgcc gcggccggca gcaccggcgc ggccagcgac acgagcaggg
       61 cgaggaggat cggggcgcgg gtcattcgat atcgtccgac ggatcgagcc gtaccaccca
      121 tcggggctgg ccgccgggcg acagcgcgat gtcgtggctg gggaccatgt cggcgccggt
      181 ctcgatccag ccgcgcccgt cgcacttggg acaggggacg cggatcgagc ggtggctgac
      241 gaggtcgatc tggctgttcc actgcccgtg gcccgcgcag accgggcaca gccggtcgag
      301 gtcgccggtg cggtggcgga ccgaaatgcc gtcgagcgcg tgcgggtcgc cggggccggt
      361 gcaatatacg atgttcgacg ggtgcgacat gggcggggtc cttttgcgcg ttacccccgc
      421 cccggccgca gcatcggggt caggagcggc gaggtgatgg tgcggatctc gccggtgatt
      481 tcgaagccga agcgccggta gagcggaatg ttgcgcgggt tcgagctttc cagccaggcg
      541 gcgcggccgt cggcgtcgat caccgcgatc gcggcctcca tcagcgcggt gccgagcccg
      601 cggccgcgat gcgcgggatc ggcggcaatc agcggcagat accagaccgg ctccttcgga
      661 tggaaatggt ccatctgttc gacgagcgcg tccatttcgg ccttgcggtg cgcggggatc
      721 tgttcgtcca tgatcgccgc catgacctcg ccgtcgggct cgacccccgg cggcagccag
      781 gtcgcgaccg cggcgccgtc gtcggcgacg aacgccgtcc cgtgctcgaa cgccgcgccg
      841 ccgaaggcgc cgaaaaattc gtggcggcgg ctcatatagg tcgcggcatc gggcgacgcc
      901 cagcgcgcga ccgggtcggc ggcaaagccg agcaccaagg tgtggagcag ccgcgccctg
      961 tccgcgggtt tgccgatcgt gattgtcgcc atggctcgcc tttcctctcc tgccgcccgc
     1021 gccgcgccgc gctcagtcgc cgggcggcgt cagcgtccag cgcccgtgct cgatatgcgt
     1081 cgtctttccg acaacgcttt ccatcagcaa gatttcccgg accgtccatt cgatcggcgg
     1141 cagtttcagc gcgccgaatg tctgcgaacc cagccggtcg ccgcgatagt tgatcgtgat
     1201 gtgcggttcg ggggtggtgc cgtccatgat cggcgccttt tcacgcagca gatggtcgac
     1261 cagcgccttc tggaacgccc gcgccccggc cagcggatcg cgcgtgcgca gcgtcaccgc
     1321 cttgcggttc tcgatccggt cgaagcggag cgggaagggt gccgcggcga agccgtccag
     1381 cgcggcgatg gtcgcgggca gccacccggg cggcgcatgg tggaggtcgt agagcgagat
     1441 gagcgtgaca tggaggaggt cgggaccgcg cccgggatcg ttgcgcggca gcgcggcgat
     1501 cgccgcctgc acttcgggcg gcggcttggc catgacgtag agcgggttgc gggcgcgcat
     1561 gggcgggagt gtagcatggc ggggaggtaa ggacgactgg tgggccgccg aggaggggcg
     1621 gaaggtcaca ggccggaagg tcacaaaggt cacgccacat acccctcctg tgtcccgcct
     1681 tccgggggct cctgcccctc cgccgcgtca tccgcctttc catcatcgcc gaaatcgacg
     1741 gtcagcgtcg tacccgcggc ggcgcggcgg gcttcttcga ggccgatctg gcgcgcgaat
     1801 tcggcgtcgg tcagcccgcc gggcgcgatg ccggccgcct tgagccgctg ca
//
LOCUS       DATMSU010000156         8949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_3049, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000156 DATMSU010000000
VERSION     DATMSU010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3049"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1028
                     /locus_tag="VN034_04095"
     CDS             144..1028
                     /locus_tag="VN034_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWV59810.1"
                     /translation="MPRLIDPSAGPALDLAEVTARLDESGVDLTDEASIARCADLLAG
                     LGRNRDFLADRVIAALKASYADQLEINRYSAQVFLLHRGARGFYLRANLWPAATDAVT
                     TASGAAAFSYGVPHDHNFSFLTTGYFGPGYVSDYYDYDAEAVDGRLGEPLNLQFVERS
                     QLSEGKLMLYRAHRDIHSQLPPESLSVSLNIMDEGEQVPWRDQYIVDLEKGTIAKRPT
                     LTSSEMLLRCAVHLTENGRDIADQFAKTHPVPRVRANAIAALAAVEEGKVRATVLERG
                     MRDSDARVRDDCARWLAS"
     gene            complement(1173..4418)
                     /locus_tag="VN034_04100"
     CDS             complement(1173..4418)
                     /locus_tag="VN034_04100"
                     /inference="COORDINATES: protein motif:HMM:NF037202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="HWV59811.1"
                     /translation="MDIAAYLDEVRAQFESGHATEHSYRPALFRLFQSIDPAITVVNE
                     PKRGDAGMVDFLFERDGVPFGWAEAKDIDKDVIRLKGYSIEQRKRYEKAYPNLIYTNG
                     VDFEFIRDGAQVHFVSIADFLMGLQPLPDKFEELERQLKLFAEVKPISIRSAAKLAEM
                     MAAKAAIIKDEIGIALADEAEARKGLEGQYKSFKANLLPNLTPDEFADIYAETITYGM
                     FAARFHDDDLNTFSRQEAMDDLPASNPFLKGLFEYIAGPALPKRLTYIVDDLVAVMRA
                     SDPHSLFEKYGQFTARNDPFIHFYEDFLAAYNPKKRKSRGVWYTPEPVVDFIVRAVDD
                     VLKTEFGLADGLADTSKVTVDWDTGTNDPKTGKAVTIKKEVHKVQILDPATGTGTFLA
                     KAVQLISDRVKARAPGKWSGYVEQDLLPRLHGFELLMASYAMCHMKLDMQLEQTGYKP
                     SANPPRLSVWLTNALEPAEREVRDLFFAPLAEEARGASEVKRQTPIMCVIGNPPYSGE
                     SSNKGDHIMALMEAYKKEPGGKEKLKERNPKWINDDYVKFIRMTEDLIAKNPSGGVLG
                     FITNHGYLDNPTFRGMRWHLLRTFDKIWVIDLHGNSKKQEVSPDGSPDKNVFDIQQGV
                     ALIIAARKGGGSEDLAKLWHGDVWGTRQTKNDILWTSERHEVAATLLPPKPNEFPFEY
                     RNEALEQTYLAGVALNQFFPQNAIGIITKRDAAVLNERRTVIEALVEDLRFQTLDEVR
                     RRHPEIEDVAGWSLASAQQNVISTSESGRIERILNFPFDIKYTYHSDQSLGFLARPVF
                     PTMRHFYLSNIGLMFTRMTKDDFSIFVSSHMATHKSGSRYDGTYVAPLYLYPAEGTLD
                     QTIRLNIDPKLHAAICKAAEIDPASSAGFDDDFGAATGDARPSEIKVFDYIYGVLHAP
                     NYRETYAEFLKIDFPRIPYPKSPEVFAHVSEKGEALRRLHLMEAAAIGETPYRYDGPD
                     DGDDVVASGFPKWEKSGTPDQVRGDEGVSTTLDTNGVLGRVFINADQYFEDVPETAWN
                     FHIGGYQPAQKWLKDRRGQALSFEDIVHYQKIVKILIETDRIMRAIELPLE"
     gene            4850..6673
                     /gene="lepA"
                     /locus_tag="VN034_04105"
     CDS             4850..6673
                     /gene="lepA"
                     /locus_tag="VN034_04105"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWV59812.1"
                     /translation="MTTPLSHIRNFSIIAHIDHGKSTLADRLIQFTGGLTAREMSAQV
                     LDNMDIEKERGITIKAQTVRLKYKAHDGETYELNLMDTPGHVDFAYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYQSIEHDHEIVPVINKIDLPAADVDKVKAEIEDIIGLPAD
                     DAVLASAKAGIGIEEALEAIVTKIPPPKGDAAAPLLAMLVDSWYDPYLGVVILVRVVD
                     GVLKKGQQIKFMQAGTTHLIDRVGCFTPKREELTEIGAGEIGFITAQIKDVAQARVGD
                     TVTDAKKPAAAPLPGFKEVQPVVFCGLFPTDANDFEKLRESIQKLRLNDASFSFEMES
                     SAALGFGFRCGFLGLLHLEIIQERLTREYDLDLITTAPSVVYKLKLGHTKNEAAKEIE
                     LHNPADMPDPNRIDEIEEPWIEAVIYVPDDYLGPILKLCQDRRGVQKNLTYVGGRAQI
                     TYELPLNEVVFDFYDRLKSISRGYASFDYHQIGHRAGDLVKMSILVNNEPVDALSMIV
                     HRGSAESRGRGMCERLKDLIPRHMFKIPIQAAIGGKVIARETIAALRKDVTAKCYGGD
                     ATRKKKLLEKQKEGKKRMREYGNVNIPQEAFIAALRMGDDA"
     gene            complement(6686..8533)
                     /locus_tag="VN034_04110"
     CDS             complement(6686..8533)
                     /locus_tag="VN034_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HWV59813.1"
                     /translation="MFAIDSLLVKIALIGIIGLGAQWIAWRTGRPSIALMLIAGIVAG
                     PILGFINPERDFGALREPIIKLAVAVILFEGGLSLKFRELRQAGTAVLMLVFIGVPLG
                     WALGTAAAYYGAGLPLELAALFGGVMVVTGPTVIAPLLRSLNIPPRVKHMLKWEAIVN
                     DPIGALIAVGVYSYITHGGGAAASSVVLDVAAASLLAILIGTAAGFALTTAYLRGWIP
                     EYLKAPILLTSVIAVFVLSDLVMHETGLITVTAMGVVMANRETYSSHMLRRFKEDLTI
                     LLVSGVFIILSATLDWQVVRNFELRFVLFLLLLLFVVRPLTIMTALLFTRIPLRERLF
                     VAWIAPRGIVAVAVTGLFALRLTDYGVPGAEALVPLGFGVVIVTIFAHGFTAGAVARL
                     LDLDRGKGDGVLLVGANSWTIAFAEFVKAQGRNVLIADEDKLALRRARRADIPVYHGD
                     ILDEAHEDEIELGQYRQLIAATGSDSYNALVCSELAPEIGADRVSRTGGQSRLKGLRR
                     GRIFTLAGTPIEDLLDRLQAGWNFGRTRITEKFTYDDFVARMKANGGDSLAVAKASGE
                     LVIFSSERRPTVEPEDVIWTFVPPETAPAPGASASAGNAASATAPAVTR"
BASE COUNT         1713 a         2858 c         2727 g         1651 t
ORIGIN      
        1 cccgaggtgc gccgcgccgc cgcggcgacc gcggcgctca tctttccgcc ttccggtgag
       61 acggggcggg aagggggtgg agggacgggg ccttgcacac gcccggcgct ctccacccca
      121 tatcgcgcag actgcccaca accatgcccc gcctgatcga cccgtccgcc ggccctgcac
      181 tcgacctcgc cgaagtcacc gcgcgcctcg acgaaagcgg cgtcgacctc accgacgaag
      241 cgagcatcgc gcgctgcgcc gacctcctcg ccgggctcgg gcgcaaccgc gactttctcg
      301 ccgaccgtgt gatcgccgcg ctcaaggcaa gctacgccga ccagctcgaa atcaaccgct
      361 attcggcgca ggtcttcctc ctccaccgcg gcgcgcgcgg cttttatctc cgcgccaacc
      421 tctggcccgc cgcgaccgac gccgtcacga ccgcgagcgg cgccgcagcc ttctcctacg
      481 gcgtcccgca cgaccataat ttcagctttc tcacgacggg ttatttcggc cccggctatg
      541 ttagcgacta ttatgactat gacgccgaag cggtcgacgg ccgcctcggc gagccgctga
      601 acctgcaatt cgtcgagcgc agccagctaa gcgaaggcaa gctgatgctc taccgcgccc
      661 accgcgacat ccacagccag ctcccgcccg aaagtctgtc ggtcagcctc aacatcatgg
      721 acgagggcga gcaagtcccc tggcgcgacc aatatatcgt cgacctcgag aagggcacga
      781 tcgccaaacg cccgacgctg accagcagcg aaatgctgct ccgctgcgcg gtgcatctga
      841 ccgagaatgg tcgggacatc gccgaccagt tcgcaaagac gcacccggtg ccaagagtcc
      901 gcgccaacgc catcgccgcg ctggcggcgg tggaggaagg caaggtcagg gcgacggtgc
      961 tggaacgcgg gatgcgcgat agcgacgcgc gggtgcgcga tgattgcgcg cggtggctgg
     1021 caagctgatc ctccctgtgg cgaagccacg gggagggcga ccgcccgaag ggtggtggag
     1081 gggccacgac cgtagcgcaa aacgcccctc cgtcagtgct tcgcactgcc acctccccat
     1141 ggctgcgcca cagggaggat tgcggccaac cttcattcca gcggcaattc gatcgcgcgc
     1201 atgatccggt cggtctcgat caggattttg acgatctttt gataatggac gatgtcctcg
     1261 aacgacagcg cctgcccgcg ccggtccttg agccacttct gcgccggctg gtatccgccg
     1321 atgtgaaaat tccatgcagt ctcgggcacg tcctcgaaat attggtcggc gttgatgaaa
     1381 acgcgtccca ataccccgtt cgtgtcgagc gtagtcgaga caccctcgtc accccggacc
     1441 tgatccgggg tcccgctctt ctcccacttc ggaaatccgc tcgccaccac atcgtcgccg
     1501 tcatcggggc cgtcataacg atagggcgtt tcgccgatcg ccgccgcttc catcaggtgc
     1561 aaccgccgca acgcctcgcc cttctcgctg acatgcgcaa acacctcagg cgatttgggg
     1621 tagggaatgc gcggaaaatc gatcttcaga aactcggcat aggtctcgcg atagttcggc
     1681 gcgtggagca cgccatagat atagtcgaag accttgatct cgcttgggcg ggcatcgccg
     1741 gtggcggcgc cgaagtcgtc gtcaaatccc gctgaactcg cgggatcgat ctctgctgcc
     1801 ttgcaaatgg cggcgtggag tttggggtca atgtttaggc gaatagtttg gtccagcgtt
     1861 ccctcggcgg ggtagagata gagcggggcc acatatgtgc cgtcgtagcg agaccccgac
     1921 ttatgcgttg ccatatggga tgacacgaaa atcgaaaaat catctttcgt cattcgggta
     1981 aacattaagc caatattcga taagtagaag tgccgcattg tcgggaagac aggtcgagcg
     2041 agaaatccta aggattggtc tgagtgatag gtatatttga tgtcgaatgg aaaattcaaa
     2101 attcgttcga ttctcccact ttctgatgtc gagataacat tctgctgagc gctcgcgagt
     2161 gaccatccgg ctacatcttc aatttcagga tggcgccgtc gtacctcgtc caaagtttga
     2221 aaccgcaaat cttccacgag cgcttcgatc accgtgcggc gctcattcag cacggccgca
     2281 tcacgtttag tgatgatccc aatggcgttt tgagggaaaa attgattcag cgcaacgccg
     2341 gccaagtagg tttgttcgag cgcttcattg cgatattcga atggaaattc gtttggttta
     2401 ggcggcagca acgttgcggc cacttcatgt ctttcgctgg tccacagaat gtcgtttttg
     2461 gtctggcgag ttccccagac gtctccgtgc caaagcttag ccaaatcctc gcttccgccg
     2521 ccctttcgag ccgcaattat gagggcaacg ccttgctgga tatcaaatac atttttatct
     2581 ggcgacccgt ctggactgac ctcctgcttc ttcgagttcc catgcagatc gatcacccaa
     2641 atcttgtcga aggtcctgag cagatgccaa cgcattccgc gaaaggtcgg gttgtcgaga
     2701 tatccgtgat tggtgataaa acctaatact ccgccggacg gatttttcgc gatcaagtcc
     2761 tccgtcatgc gaataaactt aacataatcg tcattgatcc atttcgggtt gcgttccttc
     2821 agcttctcct tgccgccggg ctccttcttg taggcctcca tcagcgccat gatgtggtcg
     2881 cccttgttgg aactttcgcc cgaataaggt ggatttccaa tcacgcacat gattggcgtc
     2941 tgccgcttaa cctcgctcgc gccccttgct tcctcggcca acggcgcgaa gaacaggtcg
     3001 cgcacctcgc gctcggccgg ttccagtgcg ttggtcagcc acaccgacag gcgtggtgga
     3061 ttggcactcg gcttgtagcc ggtctgttcg agctgcatgt ccagcttcat atggcacatg
     3121 gcgtaactcg ccattagcaa ttcgaacccg tgcaggcgcg gcagcagatc ctgctcgaca
     3181 tagcccgacc atttgcccgg tgcgcgcgcc ttcacccggt ccgaaatcag ctgcaccgcc
     3241 ttcgccagaa aggtgcccgt tcccgtcgcc gggtcgagaa tctggacctt gtggacctcc
     3301 ttcttgatcg tgaccgcctt gccggtcttc gggtcgttgg tgccggtgtc ccaatcgacg
     3361 gtcaccttgc tggtgtcggc gaggccgtcg gccagcccga attcggtctt cagcacatcg
     3421 tcgactgcgc ggacgatgaa atcgaccacc ggctcgggcg tgtaccagac gccgcggctc
     3481 ttgcgcttct tggggttata ggcggcaaga aaatcttcgt agaagtggat gaaggggtcg
     3541 ttgcgcgcgg tgaattgccc gtatttctcg aatagcgaat gcggatcgct cgcgcgcatc
     3601 accgcaacca aatcgtcgac gatgtaagtt aagcgcttcg gcagcgctgg tcctgcgata
     3661 tattcgaaca ggcctttcaa gaatggattg gatgcgggca gatcgtccat cgcttcctgc
     3721 cggctgaacg tgttcaggtc atcgtcgtgg aagcgcgcgg cgaacatgcc gtaagtgatc
     3781 gtttcggcat aaatgtcggc aaattcgtcg ggcgtcagat tgggcagcag attcgccttg
     3841 aagctcttat attgtccttc caaccccttg cgcgcctccg cctcgtcggc gagcgcaatc
     3901 ccgatctcgt ccttgatgat cgccgctttc gccgccatca tttcggcgag tttcgccgcc
     3961 gatcggatgc tgatcggttt cacttcggcg aagagtttga gctggcgctc cagctcctcg
     4021 aacttgtcgg gaaggggctg caaccccatc agaaaatcgg cgatcgatac gaagtgaacc
     4081 tgcgccccgt cgcggatgaa ctcgaaatct accccgttgg tatagatcag attgggatag
     4141 gccttttcgt agcgcttgcg ctgctcgatc gaatagccct tcagccgtat gacgtctttg
     4201 tcgatgtcct tggcttcggc ccagccgaac ggcacgccgt cgcgctcgaa caggaaatcg
     4261 accatgcctg cgtcgccgcg cttcggctcg ttgacaacgg tgatcgccgg atcgatgctc
     4321 tgaaacagcc tgaagagtgc gggacgatag ctatgctcgg tcgcatgccc gctctcgaac
     4381 tgcgcgcgca cctcatccag ataagccgca atatccattc gtcttttcgc cccctatgcg
     4441 tcgcgctcat gttcgcgcac agtggcgacc atagcggatc ggggcaagtc cctccgcgct
     4501 ctccgcgtct ccgcgcgaaa aacccctttc ctacattatc ccgccgtgcc agcctcgcgt
     4561 ttccggctct ttgactcgtc caaatccgct gtcccgccgc gcccgcgaaa tatcgcgccc
     4621 gcgtgcgacc tttgcgatgt ttcgcccgtc cgcgccgtgg gtgactttcg cgacctttcg
     4681 ccgccggacg cgcccggcgc accatcccac gggtatcatg ttaccgaagg ggaatgacaa
     4741 gtttgacaac ttcgcaagga agttgcgcca tcggcccctt gccggtgcgg cttcggcacc
     4801 tcgcgactca cctgctgaca gcgccgcaaa cccctgctaa cggcgcccga tgacgactcc
     4861 cctttcgcat atccgcaatt tttcgatcat cgcgcacatc gaccatggca agtcgacgct
     4921 cgccgaccgg ctgatccagt tcaccggcgg cctcaccgcg cgcgagatgt cggcgcaagt
     4981 ccttgataat atggacatcg agaaggagcg cgggatcacg atcaaggcgc agaccgtgcg
     5041 cctgaaatat aaggcgcacg atggcgagac ctatgagctc aacctgatgg acacgccggg
     5101 gcacgtcgac ttcgcctatg aagtgtcgcg gtcgctcgcc gcgtgcgagg gcgcgctgct
     5161 cgtcgtcgac gccgcgcagg gggtcgaggc gcagacgctg gccaacgtct atcagtcgat
     5221 cgagcatgac catgagatcg tgccggtgat caacaagatc gacctgcccg cggccgacgt
     5281 cgacaaggtg aaggccgaga tcgaggacat catcggcctg cccgccgacg atgcggtgct
     5341 cgcgtcggcg aaggccggga tcggcatcga ggaagccctc gaggcgatcg tcacgaagat
     5401 cccgccgccg aagggcgacg ccgcggcgcc gctgctcgcg atgctcgtcg acagctggta
     5461 cgacccgtat ctgggcgtcg tcattctcgt ccgcgtcgtc gacggcgtgc tcaaaaaagg
     5521 ccagcagatc aagttcatgc aggcgggcac cacccacctg atcgaccgcg tcggctgctt
     5581 cacgccaaag cgcgaggagc tgaccgagat cggcgccggc gagatcggct tcatcaccgc
     5641 gcagatcaag gacgtcgcgc aggcgcgggt cggcgacacg gtgaccgacg ccaagaaacc
     5701 cgccgcggcg ccgctgccgg ggttcaagga agtccagccg gtggtcttct gcggcctgtt
     5761 cccgaccgac gcgaacgatt tcgagaaact ccgcgagagc atccagaagc tccgcctcaa
     5821 cgatgcgagc ttcagtttcg agatggaaag ctcggccgcg ctcggcttcg gcttccgctg
     5881 cggttttctc ggcctgctgc acctcgagat catccaggag cggctgacgc gcgaatatga
     5941 tctcgacctg atcaccaccg cgccgtcggt ggtctacaag ctcaagctcg gccacaccaa
     6001 gaatgaggcg gcgaaggaga tcgagctcca caaccccgcc gacatgcccg atcccaaccg
     6061 catcgacgag atcgaggagc cgtggatcga ggcggtgatt tacgtccccg acgattatct
     6121 cggcccgatc ctgaagctgt gccaggaccg ccgcggggtg cagaagaacc tcacttacgt
     6181 cggcggccgc gcgcagatca cctacgaact gccgctcaac gaggtggtgt tcgacttcta
     6241 cgaccggctg aagagcattt cgcgcggcta tgcgtcgttc gactatcacc agatcggcca
     6301 ccgcgccggc gacctcgtca agatgagcat cctcgtcaac aacgagccgg tcgacgcgct
     6361 gagcatgatc gtccaccgcg gcagcgcgga aagccgcggc cgcggcatgt gcgagcgcct
     6421 caaggacctg atcccgcgcc acatgttcaa gatcccgatc caggccgcga tcggcggcaa
     6481 ggtcatcgcc cgcgaaacca tcgccgcgct gcgcaaggac gtgaccgcca aatgctacgg
     6541 cggcgacgcg acccgcaaga aaaagctgct ggagaagcag aaagagggca agaagcggat
     6601 gcgcgagtat ggaaacgtca acattccgca ggaggcgttt atcgcggcgc tgcggatggg
     6661 ggatgatgcc tgatccggaa agactctagc gcgtaaccgc cggcgcggtt gccgaagcgg
     6721 cgttgcccgc gctggccgaa gcgcccggtg caggggccgt ttcgggcggc acaaacgtcc
     6781 agatcacgtc ctcgggctcg acggtcgggc gccgctcgct cgagaagatg accaactccc
     6841 ccgacgcctt ggcaacggcg aggctgtcgc cgccgttcgc cttcatccgt gcgacgaaat
     6901 cgtcataggt gaacttctcg gtaatccgcg tccgcccaaa gttccagccc gcctgcaggc
     6961 ggtcgaggag atcctcgatc ggcgtgccgg caagcgtgaa aatccggccg cggcgcaggc
     7021 ccttgaggcg cgactggcca ccggtgcggc tgacgcgatc ggcgccgatc tcgggggcaa
     7081 gctcgctgca gacgagcgca ttatagctgt cgctgccggt cgccgcgatc agctggcgat
     7141 actggccgag ttcgatctcg tcctcatgcg cctcgtcgag aatatcgcca tgatagacgg
     7201 gaatatcggc gcggcgcgcg cggcggagcg cgagcttgtc ttcgtcggcg atcagcacgt
     7261 tgcgcccctg cgccttcacg aattcggcaa aggcgatcgt ccagctgttg gcgccgacga
     7321 gcagcacgcc gtcgcccttg ccgcgatcga gatcgagaag ccgcgccacc gcccccgccg
     7381 taaagccgtg cgcgaagatc gtcacgatca cgacgccaaa gcccagcggc acgagcgcct
     7441 cggcgcccgg aacgccatag tcggtgagcc gaagcgcgaa gagaccggtc accgcgactg
     7501 cgacgatgcc gcgcggggcg atccaggcga cgaacagccg ttcgcgcagc gggatgcgcg
     7561 tgaaaagcag cgcggtcatg atcgtgagcg gacgcacgac gaagaggagg agcagaagga
     7621 acaggacaaa gcgcagctcg aagttgcgga cgacctgcca gtcgagcgtt gccgacagga
     7681 tgatgaagac gcccgacacg agcaggatcg tcagatcctc cttgaaccgc cggagcatgt
     7741 gactcgaata ggtttcgcga ttggccatca cgacccccat cgccgtgacc gtgatcagcc
     7801 ccgtctcgtg catcacgagg tccgacagga cgaagaccgc gatcaccgac gtcagcagga
     7861 tcggcgcctt cagatattcg ggaatccagc cgcggagata ggcggtggtg agcgcaaagc
     7921 cggccgcggt gccgatcagg atggcgagca ggcttgcagc ggcgacatcg aggaccaccg
     7981 aactcgctgc cgcgccgccg ccgtgggtga tatagctgta gacgccgacc gcgatcagcg
     8041 cgccgatcgg atcgttcacg atcgcttccc acttgagcat atgcttgacg cgcggcggaa
     8101 tgttgagcga acggagcagc ggcgcgatga cggtcggccc cgtgacgacc atcacgccgc
     8161 caaagagcgc cgccagttcg agcgggaggc cggcaccata atatgcggcg gcggtgccga
     8221 gcgcccagcc gagcggcacg ccgatgaaca cgagcatcag cacggcggtt cccgcttgtc
     8281 gaagctcgcg gaatttgagg ctgagaccgc cctcgaacag gatcaccgcc acggcgagct
     8341 tgatgatggg ttcgcgcagc gcgccaaagt cgcgctcggg attgatgaag ccgagaattg
     8401 ggcctgcgac gatgcccgcg atcagcatca gggcgataga gggccgcccc gttcgccacg
     8461 cgatccactg ggcgccgaga ccgatgatgc cgatgagggc gattttgacg agcagcgagt
     8521 cgatggcgaa catatgggca agctatgcgg gaagggcgct atggttcccg tttggacgcg
     8581 caattggcgc acgtccagca cgatcgggaa tacctcgcgc agaacaggcg gcgaattcgc
     8641 ctgccatcgt cgccattgcc gcaaacggcc gccgcaaagg gccggatgct gccgggcgtg
     8701 ttcgaggcgc ggcgccgcgg gtcgagatcg gaaatgggga aaagcccggg ctttggaggc
     8761 cccgcctttc atggtaggga aaggcgctgc ttcggttcgc cttggggaag gggaattgtc
     8821 atgcgtcatc acttccggat cgcggccgtc cttggcatcg ggctgtcggt cgcgagtttt
     8881 gcgccgcccg cgggcttttc ggcggcgacg ctcgatgcgc tcgacccgag ccggctcgcc
     8941 ggcctgtcg
//
LOCUS       DATMSU010000157         5281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_163750,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000157 DATMSU010000000
VERSION     DATMSU010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163750"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VN034_04115"
     CDS             <1..798
                     /locus_tag="VN034_04115"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWV59814.1"
                     /translation="AQGSTRPPRLLAMRWNGGNAGEAPVVFIGKGVTFDTGGISLKPG
                     PGMDMMKWDMGGAGAVAGAIKAIAGRKAKANVVGVVGLVENMPDGNAMRPGDIVTTMS
                     GQTVEVLNTDAEGRLVLCDAISWAQKSYDPKVIVDLATLTGAMVISLGHEYAGMFAND
                     DALAAGLLAAGEASNNKLWRFPLSPAYDKLIDSPIADMKNIGPREGGSITAAQFLKRY
                     VEDGVAWAHLDIAGMAWGDKDGPVYAKGATGYGVRLLDRYIAANHEG"
     gene            830..1279
                     /locus_tag="VN034_04120"
     CDS             830..1279
                     /locus_tag="VN034_04120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HWV59815.1"
                     /translation="MPRVDFYRLTRDPVERVLPALATRILGNGDRLLVVAASAMQRQA
                     IDEALWTLQPASFLPHGHAGSPDEAIEPILISGTLDPSPPNRATLVALADGEWRDEAL
                     GFERTFLLFDNSRIDDARALWRTLAAQGDVDNRFWKQDENGRWSEGP"
     gene            1365..1787
                     /gene="ndk"
                     /locus_tag="VN034_04125"
     CDS             1365..1787
                     /gene="ndk"
                     /locus_tag="VN034_04125"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWV59816.1"
                     /translation="MAVTRTFSIIKPDATRRNLTGAVTKMLEDAGLRVVASKRIHMSR
                     EQAEGFYAVHKERPFFGELVDFMISGPVVVQVLEGENAMQANRDIMGATNPKDAAPGT
                     IRKELAESIEANSVHGSDSDENAAIEIAYFFKPEEIVG"
     gene            complement(1845..2795)
                     /gene="purN"
                     /locus_tag="VN034_04130"
     CDS             complement(1845..2795)
                     /gene="purN"
                     /locus_tag="VN034_04130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWV59817.1"
                     /translation="MTKAKVAVLISGAGTNMAALLYAAKAETCPYELVLVASNDPDAA
                     GLKIAEAEGIATWARTHKGMSRDAFDALVDEQLRAAGAEYIALAGYMRILSDDFVARW
                     AGRMINIHPSLLPLYKGLNTHSQAIEAGDKFGGCSIHIVTPGVDEGPVLAQTPVAILP
                     GDSVETLARRVQFAEHQLYPATLAAFVTRERSPDYLRGRVRELAMGLPEADEVTSHGM
                     PCFGIVKGKKFAYFTEDHHGDGKIALLVKISGGDEQAALIELDEERYYRPAYFGDGWV
                     GIRLDLGDTDWDAIGEWLRKSWLSVAPKKLAGLMAVAEDF"
     gene            complement(2911..4017)
                     /gene="purM"
                     /locus_tag="VN034_04135"
     CDS             complement(2911..4017)
                     /gene="purM"
                     /locus_tag="VN034_04135"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWV59818.1"
                     /translation="MDSKHNQPASYTYAEAGVSIETGNALVRAIAPLARATRRPGADA
                     DLGGFGGFFDLKAAGFNDPLLVAANDGVGTKLKLAIESGKHDGVGIDLVAMCANDLIV
                     QGAEPLFFLDYYATGKLDNAVATEVVASIAEGCKQAGCALIGGETAEMPGMYSDGDYD
                     LAGFCVGAVERDQVLTADKVAAGDVILGLASSGVHSNGFSLVRRLAVDKGWKLDRPAL
                     FDQNVLLIDALMAPTRIYVKSLLPLVKTGRIHALAHITGGGLLENIPRILPKTLHAHI
                     DADAWEQPRLMAYLQAQGNIEPEEMARTFNCGIGMAVVVAEDDAADVTAALEAAGETV
                     VRIGHIAAGEKGCTVTGSAETWSAMGAWSATHHG"
     gene            4103..>5281
                     /locus_tag="VN034_04140"
     CDS             4103..>5281
                     /locus_tag="VN034_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HWV59819.1"
                     /translation="MISAAFPRFAPSFSFDALRGRDWRWAALFTLFLAILFAGIVDGQ
                     ITRGDRGITPINSSGDFLASGILVDVTGDNADDARSKGWREAQRKGWAQLYRRLNGTD
                     GPALGDSVLDGIVTAIVVEEEQIGPRRYVAKLGIQFDRVRAGQILGVSGRTLRSPPLL
                     VIPVYSIDAIPQVFEQRSAWQRAWAEYNTGQSAIDYVRTAGTGADTLLINTGQTGRRG
                     RVWWRVILDQYGAADVLIPMARVEYSYPGGPIKGTFTARFGPDSKLISSFTMTGPSPA
                     ALPDMMEKAVARMDRIYTDALTAGILKTDTYLVLEKPVEKEDLPEEVATDEELLPSEI
                     DASIPTAPAAGVQSFTVQYSSPDVDSVTATERAVAGVAGVQSASTTSLALGGTSVMRV
                     S"
BASE COUNT          924 a         1816 c         1657 g          884 t
ORIGIN      
        1 gcgcagggtt cgacgcgtcc gccgcggctg ctcgcgatgc gctggaacgg cggcaatgcg
       61 ggtgaggcgc cggtggtgtt catcggcaag ggcgtgacct tcgacaccgg cggcatcagc
      121 ctcaagcccg gcccgggcat ggacatgatg aaatgggaca tgggcggcgc gggcgcggtc
      181 gcgggtgcga tcaaggcgat cgcggggcgc aaggcgaagg cgaacgtggt cggcgtcgtc
      241 gggctcgtcg aaaatatgcc cgacggcaat gcgatgcgcc ccggcgacat cgtcaccacc
      301 atgtcgggcc agacggtcga ggtgctcaac accgacgccg agggccgcct cgtcctctgc
      361 gacgcgatca gctgggcaca aaaatcctat gatcccaagg tgatcgtcga cctcgcgacg
      421 ctgaccggtg cgatggtgat ctcgctcggc cacgaatatg ccggcatgtt cgcgaacgac
      481 gacgcgctcg ctgccggcct gctcgcggcg ggcgaggcgt cgaacaacaa gctgtggcgc
      541 ttcccgctct cgcccgccta tgacaagctg atcgacagcc cgatcgccga tatgaagaat
      601 atcggcccgc gcgaaggcgg ctcgatcacc gcggcgcaat tcctgaaacg ctatgtcgag
      661 gacggcgtcg cctgggcgca tctcgacatc gcgggcatgg catggggcga caaggacggc
      721 ccggtctatg ccaagggcgc gacgggctac ggcgtgcgcc tgctcgaccg ctatatcgcc
      781 gcgaaccacg aaggctgatc gggccggaaa gaaggatagg gaggggagcg tgccgcgcgt
      841 cgacttctac cggctgaccc gcgaccccgt cgaacgcgtg ctccccgcgc tcgcgacgcg
      901 catcctcggc aatggcgacc gtctgctggt ggtcgccgct tcggcgatgc agcgccaagc
      961 gatcgacgag gcgctgtgga cgctccagcc cgccagcttt ctgccgcacg gccacgccgg
     1021 atcgcccgac gaggcgatcg agccgatcct gatttcgggc acgctcgacc cctcgccccc
     1081 caaccgggca accctcgtgg cacttgccga cggcgaatgg cgcgacgagg cgctggggtt
     1141 cgagcgcacc ttcctcctct tcgacaacag ccgcatcgac gacgcccgtg cgctctggcg
     1201 cacgctcgcg gcgcagggcg atgtcgacaa ccgcttctgg aaacaggacg aaaacggccg
     1261 ctggtccgaa gggccttaga tctccgccgc tctgtccttg caggacggcg aaagcgccgc
     1321 tagaggcgcg cgcatcatat aacacagcaa tcaggagctt tcccatggcg gtcacccgca
     1381 ctttctcgat catcaagccc gacgccacgc gccgcaatct caccggcgcc gtcaccaaga
     1441 tgctcgaaga cgccggcctc cgcgtcgtcg cctcgaagcg catccacatg agccgcgaac
     1501 aggccgaagg cttctacgcg gtccacaagg aacgcccctt cttcggcgaa ctcgtcgatt
     1561 tcatgatcag cggtccggtc gtcgtgcagg tgctcgaagg cgaaaacgcc atgcaggcga
     1621 accgcgacat catgggcgcg accaacccca aggacgccgc gcccggcacg atccgcaagg
     1681 aacttgccga gagcatcgag gcgaacagcg tccacggtag cgacagcgac gaaaatgccg
     1741 cgatcgaaat cgcctatttc ttcaagcccg aagaaatcgt cggctaaacg gctcttgccg
     1801 cctggcacga agaggccgcc ggagcgatcc ggcggccttt ttcgtcagaa gtcttccgcg
     1861 accgccatca gccccgccag cttcttcggc gcgaccgaca gccagctctt gcgcagccat
     1921 tcgccgatcg catcccaatc ggtgtcgcca aggtcgagcc ggatgccgac ccagccgtcg
     1981 ccgaaatagg cggggcggta atagcgctct tcgtcgagtt cgatcagcgc cgcctgctcg
     2041 tcgccgccgc tgatcttgac gagcaaagcg atcttgccgt cgccgtgatg gtcctcggtg
     2101 aaataggcga atttcttgcc tttcacgatc ccgaaacagg gcatgccgtg cgaggtgacc
     2161 tcgtcggcct cgggcagccc catcgcgagt tcgcgtacgc gcccgcgcag ataatccggc
     2221 gagcgctcgc gcgtcacaaa ggcggcgagg gtggcgggat aaagctgatg ctcggcgaac
     2281 tgcacccgcc gcgccagcgt ctcgacgcta tcgccgggca ggatcgcgac cggcgtctgc
     2341 gcgagcaccg gcccctcgtc gacgcccggc gtcacgatat ggatgctgca tccgccgaac
     2401 ttgtcgcccg cctcgatcgc ttggctgtgc gtgttcagcc ccttgtagag cggcagcagg
     2461 ctcgggtgga tattgatcat ccgccccgcc cagcgcgcga cgaaatcgtc cgacagaatc
     2521 cgcatatagc ccgcgagcgc gatatattcg gcgcccgccg cgcggagttg ctcgtcgacc
     2581 agcgcgtcga acgcgtcgcg gctcatgcct ttgtgcgtgc gcgcccatgt cgcgatgcct
     2641 tcggcttcag cgatcttgag ccccgccgcg tcgggatcgt tgctcgcgac cagcaccagt
     2701 tcatagggac aggtttcggc tttcgccgca tagagcagcg ccgccatatt ggtgccggcg
     2761 cccgagatca ggacggcgac cttggctttc gtcactgcgc cactcctctg atccgttcgt
     2821 gctgagcttg tcgaagcact gttcttgtct cttcttgggt tgaaaggaag aacggccctt
     2881 cgacaagctc agggcgaacg ggttcgggaa tcacccgtgg tgcgtcgcgc tccacgcccc
     2941 catcgcgctc cacgtttcgg cgctgccggt cacggtgcag cctttctcgc ccgcggcgat
     3001 atgtccgatc cggaccaccg tttcgcccgc cgcttcgagc gccgcggtaa catcggccgc
     3061 atcgtcctcg gcaacgacga ccgccatccc gatcccgcag ttgaaggtgc gcgccatttc
     3121 ctcgggctcg atattcccct gcgcctgcag atacgccatc aaccgcggct gctcccacgc
     3181 gtcggcatcg atatgcgcat gaagcgtttt cggcaggatg cgcgggatat tctcgagcag
     3241 cccgccgccg gtgatatgcg cgagcgcgtg aatccggccg gtcttcacca gcggcagcaa
     3301 gctcttcaca tagatgcgcg tcggcgccat cagcgcgtcg atcagcagca cattctggtc
     3361 gaacagggca gggcgatcga gcttccaccc cttgtcgacc gcgagccggc ggacgagcga
     3421 aaagccgttc gaatgcaccc ccgacgaggc gagccccaaa atcacgtcac ccgccgcgac
     3481 cttgtcggcg gtcagcacct ggtcgcgctc gaccgcgccg acgcagaagc ccgcgaggtc
     3541 atagtcaccg tccgaataca tgcccggcat ttcggcggtc tcgccgccga tcagcgcgca
     3601 tccggcctgt ttgcacccct cggcgatgct cgcgacgact tcggtagcga ccgcattgtc
     3661 gagcttgccg gtcgcataat agtcgaggaa gaacagcggt tcggcgccct gcacgatcag
     3721 gtcgttcgcg cacatcgcaa ccaggtcgat cccgacgccg tcatgcttgc ccgattcgat
     3781 ggcgagcttc aatttcgtgc ctacgccgtc gttcgccgcg acgagcaggg gatcgttgaa
     3841 ccccgccgcc ttcaggtcga aaaaaccgcc aaacccgccc agatcggcat cggcgccggg
     3901 acggcgcgtc gcacgggcga gcggggcaat ggcacggacc agcgcgttgc cggtctcgat
     3961 cgatacgcca gcctcggcat aggtgtagga ggcgggttgg ttgtgcttgg aatccatggc
     4021 acgcgcgact aacggtttca gccgataaat gccatgattt ccttgtcggg gcccgcgctt
     4081 ttgggcaaag catagagaag atatgatttc ggccgctttc ccccgcttcg ccccaagctt
     4141 ttccttcgac gcgctgcgcg gccgcgattg gcgctgggcc gcgcttttca ccttattcct
     4201 tgcgattctg ttcgccggca tcgtcgatgg gcagattacc cgcggcgacc gcggcatcac
     4261 cccgatcaac agcagtggcg atttcctcgc cagcggcatt ctggtcgacg tcaccggcga
     4321 caatgccgac gacgcgcgct cgaagggctg gcgcgaggcg cagcgcaagg gctgggcgca
     4381 gctctaccgc cggctgaacg gcaccgacgg tcccgcgctc ggcgattcgg tgctcgacgg
     4441 catcgtcacc gcgatcgtcg tcgaggagga acagatcggc ccgcgccgtt atgtggcaaa
     4501 gctcggcatc cagttcgacc gcgtccgcgc cggacagata ttgggcgtca gcggccgcac
     4561 gctccgctcg ccgcctttgc tcgtgatccc cgtttattcg atcgatgcca tcccgcaggt
     4621 gttcgaacag cgcagcgcct ggcagcgcgc ttgggccgaa tataatacgg ggcagagcgc
     4681 gatcgactat gtccgtaccg cgggcaccgg cgccgacact ttgctgatca acaccgggca
     4741 gaccggccgc cgcgggcgcg tctggtggcg cgtgatcctc gatcaatatg gcgccgccga
     4801 cgtgcttatc ccgatggcgc gcgtcgaata ttcgtacccc ggcgggccga tcaaaggcac
     4861 tttcacggcg cgcttcggcc ccgacagcaa gctgatctcc agctttacca tgaccggtcc
     4921 cagccccgcg gcgttgcccg acatgatgga aaaggcggtc gcgcggatgg accgcatcta
     4981 taccgacgcg ctgaccgccg gcattctcaa gaccgacacc tatctggtgc tcgaaaagcc
     5041 ggtcgaaaag gaagatctgc ccgaagaggt cgcgaccgac gaagagctgc tgcccagcga
     5101 gatcgacgcg agcataccca ccgcgcccgc cgcgggcgta cagagcttta cggtgcagta
     5161 cagctcaccc gacgtcgatt cggtcaccgc gaccgaacgc gccgtcgccg gcgtcgcggg
     5221 cgtccagtcc gcctcgacca ccagccttgc gctcggcggc acctcggtga tgcgcgtcag
     5281 c
//
LOCUS       DATMSU010000158         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_154503,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000158 DATMSU010000000
VERSION     DATMSU010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154503"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VN034_04145"
     CDS             complement(<1..641)
                     /locus_tag="VN034_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV59820.1"
                     /translation="MEPILIYGFPLGSSMGLVAALEWLGKPYRLCRVDMLGEMREPAY
                     ARINPRHETPALITDEGKVLTETMAIAHWLAARDDERRISPDPMSAEGDRMRQIMAFI
                     NTGFTGAFTPLWVALEMDPPRPALQASLREFGKEAVIGRHDRLEEMVGDGPYLVGDRP
                     TLADALFIGVARWLEFHEVAEPVRWPKLAALRARLEADPAVAYATALESGESAA"
     gene            747..1088
                     /locus_tag="VN034_04150"
     CDS             747..1088
                     /locus_tag="VN034_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV59821.1"
                     /translation="MKDLVSRCPIEEVMQLLSGRWPTLLIYYLSDGTKRFGDLRRDNP
                     AISHKMLTLELRKLEDAGIVRRTEFEGYPRRVEYDLTVDGERLVPLIDALGDWWDKVA
                     PAPAVADAIAR"
     gene            1085..1540
                     /locus_tag="VN034_04155"
     CDS             1085..1540
                     /locus_tag="VN034_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3)-I family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HWV59822.1"
                     /translation="MIVRRLGPGDIAAARALNTVYAAAFDDPVTYRIDRPDDAWLARQ
                     LGRDGVIMVVAELGGVVVGGLTAYDLPKLEAARSEIYLYDLAVDAAHRRRGIATALIA
                     ELQHIAAETGAWAVFVQADYGDDPAVALYTKLGAREDVMHFDLLPRPRA"
     gene            1603..>2512
                     /locus_tag="VN034_04160"
     CDS             1603..>2512
                     /locus_tag="VN034_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HWV59823.1"
                     /translation="MSEKNHLYLVDGSSYIFRAYHRLPPLTNPNGVPVGAVYGYTTML
                     WKLAKDLHDADGPTHLAVILDHSSQSFRNEIYDQYKANRPEPPEDLRPQFPLIRDATR
                     AFSLPCIEMEGFEADDLIASYAEAAVREGWDVTIVSSDKDLMQLIREPADGSPQVDML
                     DTMKNVRLGLEAVNEKFGVIPDLVGDVLALMGDSVDNVPGVRGVGPKTATKLIQEYGN
                     LTAALDGAATMKASKLRDNLIEHRAMAELSRILVDLKRDCPLPDALDALKLGAIPPLP
                     LKSFLDEHGFRSLSAKLDLGGTPGGPP"
BASE COUNT          423 a          849 c          814 g          426 t
ORIGIN      
        1 gccgcgcttt caccgctttc gagcgcggtg gcataggcga ccgcgggatc ggcctcgagc
       61 cttgcgcgca gcgccgccag cttcggccag cgaacgggct cggcgacctc atggaattcc
      121 agccaccgcg cgacgccgat gaacagcgcg tcggccagcg tcggccggtc gccgaccaga
      181 taggggccgt cgccgaccat ttcctcgagc cggtcgtggc gcccgatcac cgcttccttg
      241 ccgaattcgc gcaatgatgc ctgcagcgcc gggcgcggcg ggtccatttc gagcgcgacc
      301 cacagcgggg tgaaggcgcc ggtgaagccg gtgttgatga acgccatgat ctgccgcatc
      361 cggtcgcctt ccgccgacat cggatcgggg ctgatccgcc gttcgtcgtc gcgcgcggcg
      421 agccagtgcg cgatcgccat cgtttcggtc agtaccttgc cctcgtcggt gatcagcgcg
      481 ggcgtctcgt gccgcgggtt gatgcgggca taggcgggct cgcgcatttc gccgagcatg
      541 tcgacgcggc acaggcgata gggtttgccc agccattcga gggccgcgac aaggcccatg
      601 gaacttccca gcgggaagcc atagataagg atcggttcca agttattgct ccatcttctt
      661 ctgaggggga gttgcgcagc ggtttgagat atagggcgcc ggaagcccgc gtaaattacg
      721 cacctttttg taaccatgag gcgatgatga aggatctggt ttcgcgctgt ccgatcgagg
      781 aagtgatgca gctcttgagc ggccgctggc cgaccctgct gatctattat ctcagcgacg
      841 ggacgaagcg cttcggcgat ctccgccgcg acaatccggc gatctcgcac aagatgctga
      901 cgctcgaact gcgcaagctc gaggatgcgg ggatcgtccg gcggaccgaa ttcgaaggtt
      961 atccgcggcg cgtcgaatat gatctgacgg tggacggtga gcggctcgtc ccgctgatcg
     1021 acgcgctcgg cgactggtgg gataaggtcg cccccgcacc ggccgtcgcg gacgccatcg
     1081 cgcgatgatc gtccgccggc tgggaccggg cgacatcgcc gcggcgcgcg cgctcaacac
     1141 cgtctatgcc gccgccttcg acgatcccgt cacctaccgc atcgaccggc ccgacgacgc
     1201 ctggctcgcg cgccagctcg gccgcgatgg cgtcatcatg gtggtcgccg agctgggcgg
     1261 tgtcgtcgtc ggcggcctca ccgcctatga cctgcccaag ctcgaggcgg cacgcagcga
     1321 aatctatctt tacgatctcg cggtcgatgc cgcgcaccgc cgccgcggca tcgccaccgc
     1381 gctgatcgcc gaactccagc atatcgcggc ggaaaccggc gcgtgggcgg tgttcgtcca
     1441 ggccgactat ggcgacgatc cggcggttgc actctatacc aagctcggcg cgcgcgagga
     1501 tgtgatgcac ttcgacctgc tgccgcgccc gcgcgcctga gaaagaagaa agcaggaaca
     1561 tcgcgctagg ccgcccgccc cgccgctgct acaccccgcg ccatgtccga gaagaatcat
     1621 ctctatctgg tcgatggctc cagctacatc ttccgcgcct atcaccgcct gccgccgctg
     1681 acgaacccca acggcgtgcc cgtcggtgcg gtttatggct atacgacgat gctgtggaag
     1741 ctcgcgaagg atctgcacga cgccgacggg ccgacgcatc tcgcggtcat cctcgatcat
     1801 tcgagccagt cgttccgcaa cgaaatctac gaccaatata aggcgaaccg gcccgaaccg
     1861 cccgaggatc tgcgcccgca attcccgctg atccgcgacg cgacgcgcgc cttttcgctg
     1921 ccgtgcatcg agatggaggg cttcgaggcc gacgatctga tcgcctccta tgccgaagcc
     1981 gcggtgcgcg agggctggga cgtcacgatc gtgtcgtcgg acaaggatct gatgcagctg
     2041 atccgcgagc cagccgatgg ctcgccgcag gtcgacatgc tcgacacgat gaaaaatgtc
     2101 cggctcgggc tcgaagcggt gaacgagaag ttcggcgtca tccccgatct ggtcggcgac
     2161 gtgctcgcgc tgatgggcga cagcgtcgac aacgtccccg gcgtgcgcgg cgtcgggccg
     2221 aagaccgcga ccaagctgat ccaggaatat ggaaacctca ccgccgcgct cgacggggcg
     2281 gcgacgatga aggcgtcgaa gctgcgcgac aatctgatcg agcatcgcgc catggccgaa
     2341 ttgtcgcgca tcctcgtcga cctgaagcgc gattgcccgc tgcccgacgc gctcgacgcg
     2401 ctgaagctcg gcgcgatccc gccgctgccg ctcaagagct tcctcgacga gcatggcttc
     2461 cgttcgcttt cggccaagct cgacctcggc ggcacgcccg gcggtccgcc ga
//
LOCUS       DATMSU010000159         1750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_126306,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000159 DATMSU010000000
VERSION     DATMSU010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126306"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1750
                     /locus_tag="VN034_04165"
     CDS             <1..>1750
                     /locus_tag="VN034_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV59824.1"
                     /translation="ASAVAEQPAEAAPPASADNQSEEEEYSEDEIIVTAPRLAGQLAT
                     DIRAEAELDEAAIASYGASSIEELLDALEPQTRSGRGRGGRPVILVNGRRISGFGAVR
                     NIPPEAIAKVEIFPEEVALEYGYAATERVVNFVLKPDFRQISVEAEAGIPTQGGQFKN
                     ELEPAFIAIGDNGRLNLNASWEHQTMLLESERDLVYDDPVLAAGAPARSLASANDEYV
                     IDGTLVRNIDKVTEASVSLRFDQTDSLSLLGPRNGNPDDPRRRDSRSQNWTSSASVNG
                     MLGDWRWSVTGNYADADQRTFTEQESGARDRFDSSQQSFGTNANISGSVTEGWAGPIR
                     ASITGGYAGQRFDSRTERGVDIVTTELARDTPSVFGSLTVPLLDPEYDIGNIGRLSLT
                     LNAQAERPSDFSALTSWGATLNWGVTDNLSLLASYASDEAAPSMTQLGAAPLETEGVT
                     YYDFRTGQTVLIDTITGGNPFLLAEQRRDLKVGLNWEVPMVEGLRFSINYNRNQSNDT
                     ANGFPLLTPEIEAAFPDRVIRDDDGTLLAIDQRPVNFERATNSQIRWGFNFGKSFGQN
                     EAGGGRGRSGGRSGAGG"
BASE COUNT          321 a          594 c          574 g          261 t
ORIGIN      
        1 cggcctccgc ggtggccgag cagcccgccg aggcggcccc tccggcgtcc gccgacaatc
       61 aatcggagga ggaggaatat agcgaggacg agatcatcgt cacggcgccg cgcctcgcgg
      121 gacagcttgc caccgacatc agggccgagg ccgaactcga tgaggcggcg atcgccagct
      181 atggcgcctc gtcgatcgag gaattgctcg acgcgctcga gccgcagacg cgctcgggcc
      241 gcgggcgcgg cggccggccg gtcatcctcg tcaacgggcg gcgtatttcg ggcttcggcg
      301 cggtacgcaa catcccgccc gaagcgatcg ccaaggtcga gatttttccc gaggaggtcg
      361 cgctggaata tggctatgcc gcgaccgagc gcgtcgtcaa tttcgtgctc aagcccgatt
      421 tccgtcagat ttcggtcgaa gcggaggcgg gcatcccgac gcaggggggg cagttcaaga
      481 acgaactcga acccgccttc atcgcgatcg gcgacaacgg ccggctcaac ctcaacgcga
      541 gctgggagca tcagacgatg ctgctcgaaa gcgagcgcga tctcgtttac gacgatcccg
      601 tccttgccgc cggcgcgccg gcgcgcagcc tcgcctccgc gaacgacgaa tatgtcatcg
      661 acggcacgct ggtgcgcaac atcgacaagg tgaccgaggc gtcggtcagc ctgcgtttcg
      721 accagaccga cagcctgtcg ctgctcggcc cgcggaacgg caaccccgac gatccccgcc
      781 gccgcgacag ccgctcgcag aactggacca gttcggcgag cgtcaacggc atgctcggcg
      841 actggcgctg gtcggtgacc ggcaattatg ccgatgccga tcaacgcacc ttcaccgaac
      901 aggaaagcgg cgcgcgcgac cggttcgaca gcagccagca aagcttcggc accaacgcga
      961 atatttcggg cagtgtcacc gaaggctggg cgggcccgat ccgcgcctcg atcaccggcg
     1021 gctacgccgg gcagcgcttc gacagccgga ccgaacgcgg agtggacatc gtcacgaccg
     1081 agctcgcgcg cgacacgccg agcgtcttcg gttcgctcac cgtcccgcta ctcgatcccg
     1141 aatatgatat cggcaatatc ggccgcctgt cgctgaccct caacgcccag gccgagcggc
     1201 cgagcgactt ttcggcgctg acgagctggg gggcgacgct gaactggggc gtcaccgaca
     1261 atctgtcgct gctcgcgagc tacgccagcg acgaagcggc gcccagcatg acccagctcg
     1321 gcgcggcgcc gctcgaaacc gaaggcgtga cctattacga cttccggacc ggccagacgg
     1381 tgcttatcga cacgatcacc ggcggcaacc cgttcctgct cgccgaacag cggcgcgacc
     1441 tcaaggtcgg gctgaactgg gaggtgccga tggtcgaagg gctgcgtttc tcgatcaact
     1501 ataaccgcaa ccagagcaac gatacggcga atggttttcc gctgctgacc ccggagatcg
     1561 aggcggcctt tcccgatcgc gtgatccgcg acgacgacgg gaccttgctc gcgatcgacc
     1621 agcgtccggt caatttcgag cgcgcgacca actcgcagat tcgttggggc ttcaatttcg
     1681 gcaagagctt cggccagaac gaagcgggcg gcggccgcgg gcgctctggc ggccgttcgg
     1741 gtgccggcgg
//
LOCUS       DATMSU010000160         8446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_116732,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000160 DATMSU010000000
VERSION     DATMSU010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116732"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VN034_04170"
     CDS             complement(<1..1184)
                     /locus_tag="VN034_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV59825.1"
                     /translation="MLPLASLWRIARRDLATRIRGLRLLAVCLFLGVATLAAIGSLTR
                     GITSELEVRGQTILGGDVEFSLPQREATPEEMAAFRRVGTPSATVRLRAMANDPDGEA
                     LLSELKAVDGVYPLYGTMRLESGARKGPPPPGAIWIGKDLATRLDLKVGERVKFGEKA
                     FLIDGIIAEEPDRLGEGFTLGPVAIIGLADLPATQLIQPGSLYESKYRVRLPDSANPE
                     AVGKALTAEFPDAGWDITDSSNGAPGTRRFIERMGQFLSLVGLAALVIAGIGVGNGVA
                     SYLAGKRPGLATLKVLGADSGTVARIYGLQILAVAAVSIIAGLIVGALAPAIIGAIAG
                     DVLPVKPGIAIYPLPLAVSAAYGLLIAIAFALPPLAATRHVPAAGLYRATVSGAARID
                     RRT"
     gene            complement(1185..1886)
                     /locus_tag="VN034_04175"
     CDS             complement(1185..1886)
                     /locus_tag="VN034_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV59826.1"
                     /translation="MPDAQRQSPDLAIAAHNVTLTLGSDKAPVEILRGVDLEVAAGTS
                     VALLGPSGSGKSSLMAVLAGLERANGGSINVAGLAFAAMDEDDLARARRGRIGIVLQA
                     FHLLPTMTALENVAVPLELAGIADPFGRAAAELEAVGLGHRLTHYPAQLSGGEQQRVA
                     IARAMASSPAIIFADEPTGNLDTATGHAIIELIFARRAALGATLLIITHDPELAEHCD
                     RVVVMHDGKIEERAA"
     gene            1904..2617
                     /locus_tag="VN034_04180"
     CDS             1904..2617
                     /locus_tag="VN034_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWV59827.1"
                     /translation="MKTAGWRSALTYGCVIALCQPLAACGSAEAPPASTNDKGAAKAV
                     PAIPADAPLVIAFGDSLYAGYQLGPKEGLAPQLQMALADDGIVARVQNAGVSGDTTAA
                     GRQRLTYVLDNAKAKPALVLLGLGGNDMLRGIGPDQTRANLDAMLAELQKREIPVLLT
                     GMMAAPNLGSDYANKFNPIYPELASKYEVSFYPFILDDVITDKTLMLGDNLHPNAKGV
                     KVVAEALAPLVEQTLPEAD"
     gene            complement(2614..2937)
                     /locus_tag="VN034_04185"
     CDS             complement(2614..2937)
                     /locus_tag="VN034_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HWV59828.1"
                     /translation="MTALAYIGAALAEIAGCFAFWAWLRLDKSVWWVVPGMASLALFA
                     WLLTLVDAEHAGRTYAAYGGVYIVAALLWLWAIEGAKPDRWDLIGAAVCLGGAAIILF
                     GPRGS"
     gene            complement(3024..3482)
                     /locus_tag="VN034_04190"
     CDS             complement(3024..3482)
                     /locus_tag="VN034_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWV59829.1"
                     /translation="MKKFAVAAALLTAIVATPAMAAEDGEARVEVRGGYITGSGFDDA
                     TLGIAGGYDFDLGSTAFGGAEIAGDKVLVDGADVQFSAGGRLGAKVGAAGKLYANAGY
                     TFGQIDDPYVGAGYQHKLTQNVYAKAEYRHQFIENFSDFDTFAVGVGFAF"
     gene            complement(3706..4956)
                     /gene="rlmN"
                     /locus_tag="VN034_04195"
     CDS             complement(3706..4956)
                     /gene="rlmN"
                     /locus_tag="VN034_04195"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine(37)-C2)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine(2503)-C2-)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWV59830.1"
                     /translation="MQIPGHIDPVTTGTVPLRGGNRIDLVGLTRDAIGGVLVEAGLDA
                     KAAKLRAKQIWHWIYHRGVTDFEAMTDIAKTMRPWLTDRFIIGRPTVREAQVSNDGTR
                     KWLLAAADGQEYEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLGAGEIVG
                     QVLLARDALGEWPKGTMAGFGADPEDDDADEDAAGHYTADGRMLTNIVMMGMGEPLYN
                     FDEVKGALKIVMDGDGLALSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHAVNKEIR
                     DEIVPLNRKYGIEELLQACADYPGANNARRITFEYVMLKDKNDSDADARELVRLIKQY
                     NLPAKVNLIPFNPWPGAPYECSTPERVRAFSNLIFKAGISAPVRTPRGRDIMAACGQL
                     KSAATKPTRAELDRIAEEKQAALG"
     gene            complement(5172..5915)
                     /locus_tag="VN034_04200"
     CDS             complement(5172..5915)
                     /locus_tag="VN034_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59831.1"
                     /translation="MKTSGNTILVTGGGSGIGLALAQRWHDAGNHVIVTGRNAAKLDA
                     AIAGRPNMSAISLDVTDADAIAVFAKEIVAKHPDLNILVNNAGIMSAEDASAKRDLAH
                     AEATVVTNILGPIRLIDALVDHLAARPDSAIVNLTSGLAFVPFPKAPTYSATKAAMHS
                     YSVALRAQLEGQVEVIELAPPAVRTELTPGQSTREHYMPLDDFADEVMALFAQQPTPE
                     EILVQNVLPLRTAEANGTVPQMLAMLKSL"
     gene            6031..6423
                     /locus_tag="VN034_04205"
     CDS             6031..6423
                     /locus_tag="VN034_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV59832.1"
                     /translation="MPATEKISYDPNAPVDPRVETLVNELIGRVADKWTLLVLEELED
                     HGICRFTELSRLVPGISQKMLTQTLRAMERDGLLVRTVHPVVPPKVEYCLTDLGHSLG
                     EAFCGVWIWAETNLDRIEKARSAFDARG"
     gene            complement(6428..6862)
                     /locus_tag="VN034_04210"
     CDS             complement(6428..6862)
                     /locus_tag="VN034_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV59833.1"
                     /translation="MSNPEAFPASSTPEEKRQPRQSRLVKAALACQRLGRFDVTLRNV
                     SLTGIGGQGPHILQIGERMTVFMPGHEPMLGTVRWVAGTRFGIQTDREIETMRLRAAH
                     TDQIVAADSRADFQIIPPPRVDTWRPGLTRATSLPGQFGRKR"
     gene            complement(7003..>8446)
                     /locus_tag="VN034_04215"
     CDS             complement(7003..>8446)
                     /locus_tag="VN034_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWV59834.1"
                     /translation="KLAGLAGSGKSPRGLDLATVRSRAKGGTLPAYGAISVSVPGTVD
                     GWWTMHQRYGKLPWKELFEPVIAHAEAGAPVPDMIAYYIRRSLAGFRQPSRGIEEVDN
                     AIRTYGLNDGKGPAAGQVFRNPDLARTFRLIAEGGRDVFYEGEIARTIDAYFKRIGGW
                     LRYEDLAAHKSEWIEPHKTGYRGTDVYALGANTQGIATLQMLNILEHFDLKGAGFQSA
                     LSIHLQAEAKRLAYEDRARYYADPHFSNVPVEWLISKEYAAERAKLIRPDRLLSPVHP
                     GQAPSHGDTTYFSCADKDGMMVSMIQSNFRGMGSGLVADGLGFMFQDRGQLFSLQDGH
                     PNIYAPGKRPFQTIIPGFAARGQVPWMSFGVMGGDMQPQGQAQIIINRVDYGLEIQSA
                     GDSPRWHHEGSSQSMGEDSPGLGPNGMLRLESGVPEATRRRLADIGWSLGEPDGGFGR
                     YQCVEHRMDGDTRVYAAASEMRADGCALAY"
BASE COUNT         1411 a         2725 c         2820 g         1490 t
ORIGIN      
        1 gtgcgccggt cgatgcgggc cgcgccactc accgtcgcgc ggtagagtcc ggctgccgga
       61 acgtgccgcg tcgctgcaag cgggggcagc gcaaaggcga tcgcgatgag gaggccgtag
      121 gccgcgctga cggcaagcgg cagcggatag atcgcgatgc ctggtttgac cggcagcaca
      181 tcgcccgcaa tcgcgccgat gatcgcgggc gcgagcgcgc ccacgatcag acccgcgata
      241 atcgacaccg ccgcgaccgc gaggatctgc aggccataga tgcgcgcgac ggtgccgctg
      301 tcggcgccga ggaccttcaa cgtcgcgagg cccgggcgct tgcccgcgag atagcttgcg
      361 acgccgttgc caacgccgat ccctgcgatc accagcgcgg cgaggccgac gagcgacagg
      421 aactgcccca tccgctcgat gaagcgccgc gtaccgggtg cgccgttgct gctatcggtg
      481 atgtcccagc ccgcgtcggg gaattcggcg gtcagcgcct tgccgaccgc ttcgggattg
      541 gcgctgtcgg gcaggcggac gcgatatttg ctctcgtaaa gactgcccgg ctggatcagc
      601 tgcgtcgcgg gcaggtcggc aaggccgatg atcgcgacgg ggccgagcgt gaagccttcg
      661 ccgagccggt cgggctcctc ggcgatgatc ccgtcgatta ggaacgcttt ttcgccgaac
      721 ttcactcgct cgccgacttt gaggtcgagc cgcgtggcga ggtccttgcc gatccagatc
      781 gcgcccggcg gcggcgggcc tttgcgcgcg ccgctctcca gccgcatcgt gccgtagagc
      841 ggatagacgc cgtcgaccgc cttcagttcg gacagcagcg cctcgccgtc gggatcgttc
      901 gccatcgcgc gcagtcgcac cgtcgccgag ggcgtgccga cgcggcggaa ggccgccatt
      961 tcttcgggcg tggcctcgcg ctgcggcagg ctgaactcga catcgccgcc aaggatcgtc
     1021 tgcccgcgca cctcgagttc ggaggtgatg ccgcgcgtca ggctgccgat cgcggcgagc
     1081 gtcgcgaccc cgaggaagag gcagaccgcg agcaggcgca gcccgcgaat ccgcgtcgca
     1141 aggtcgcgcc gcgcgatccg ccagagcgag gcgaggggga gcatctaggc cgcccgctct
     1201 tcgatcttgc cgtcgtgcat cacgacgacg cggtcgcaat gttcggcgag ttcggggtcg
     1261 tgggtgatga tcaggagggt ggcgcccagc gccgcgcggc gcgcgaagat cagctcgatg
     1321 atcgcatgac ccgtcgcggt gtcgagattg cccgtcggct cgtcggcaaa gatgatcgcg
     1381 ggggaacttg ccatcgcgcg cgcgatggcg acgcgctgct gttcaccgcc cgatagctgc
     1441 gcgggataat gggtgaggcg atggccgagg cctaccgcct caagctccgc ggcggcgcgg
     1501 ccgaaggggt cggcgatccc tgccagttcg agtggcaccg cgacattttc gagcgccgtc
     1561 atcgtcggga gcaaatggaa ggcctgaagc acgatgccga tgcggccgcg ccgcgcgcgc
     1621 gccaggtcat cctcgtccat cgccgcgaaa gcgagccccg cgacgttgat gctgccgccg
     1681 ttggcgcgct cgagccccgc gagcaccgcc ataagcgagc tcttgcccga tcccgacggg
     1741 ccgagcagcg cgaccgacgt gcccgccgcg acctccaggt cgacgccgcg caaaatttcg
     1801 acgggggcct tgtcgcttcc aagcgtgagc gtcacattat gggcggcgat cgccagatcg
     1861 ggcgattgtc gttgggcgtc gggcaaggag aggacccttt ggaatgaaaa cggccggatg
     1921 gcgttctgcc ttgacatatg gttgcgtgat cgcgctttgc caaccgctcg cggcgtgcgg
     1981 gtcggcggag gcgccaccag catcgacgaa cgacaagggc gcggcgaaag cggtgcccgc
     2041 aatcccggcc gacgcgccgc tcgtcatcgc tttcggcgac agcctttatg ccggctacca
     2101 gctcggcccg aaggaggggc tggcgccgca attgcagatg gcgcttgccg atgacggcat
     2161 cgtcgcgcgc gtccagaatg ccggggtttc gggcgacacc accgccgccg ggcgtcagcg
     2221 gcttacctat gtgctcgaca atgccaaggc gaagcccgcg ctcgtgctgc tcgggctagg
     2281 cggcaacgac atgctgcgcg gcatcggccc cgaccagacg cgcgccaatc tcgacgcgat
     2341 gctcgcggaa ctgcaaaagc gcgagattcc ggtgctgctc accggcatga tggcggcgcc
     2401 gaaccttggc agcgactatg cgaacaagtt caatcccatc tatcccgaac tggcgtcgaa
     2461 atatgaggtg agtttctatc ccttcattct cgacgatgtg atcaccgaca agacgctgat
     2521 gctcggcgac aatctccacc ccaatgccaa gggcgtgaag gtggtggctg aggcgctggc
     2581 gccgctggtg gaacagacgc tgccggaggc ggactagctc ccccgcggcc cgaacaatat
     2641 gatcgccgcg ccgccgaggc acacggcggc gccgatcagg tcccagcggt cgggcttcgc
     2701 gccctcgatc gcccacagcc acagcagcgc cgcgacgata tagaccccgc cataagcggc
     2761 gtaggtgcgc ccggcgtgtt cggcatcgac gagcgtgagc agccaggcga acagcgcgag
     2821 cgacgccatt ccgggcacga cccaccatac cgacttgtcc agccgcagcc acgcccagaa
     2881 ggcgaagcag cccgcgattt ccgccagcgc cgcgccgata tatgccaatg ctgtcatcgc
     2941 cgcaccgtgg ccggagtgcg gacaaaagaa aaggggcgac ccggcgggcc gcccctctct
     3001 tttgcgtccc tggtccgaac ggatcagaaa gcgaagccga cgccgaccgc gaaggtgtcg
     3061 aagtcgctga agttctcgat gaactggtgg cgatattcgg ccttggcgta gacgttctgg
     3121 gtcagcttgt gctggtaacc ggcaccgaca tagggatcgt cgatctggcc gaaggtgtag
     3181 ccggcgttgg catagagctt gcccgcggcg ccgaccttgg caccgagacg gccgccagcc
     3241 gagaactgga catcggcgcc gtcgacgagg accttgtcgc cggcgatttc ggcgccgccg
     3301 aacgcggtcg agccgaggtc gaaatcatag ccgccggcga tgccgagcgt cgcgtcgtcg
     3361 aaaccgctgc cggtgatgta gccgccgcgc acttcgacgc gggcttcgcc gtcttcggca
     3421 gccatggcgg gggttgcaac gatggccgtc aggagagcgg ctgcaactgc gaatttcttc
     3481 atgttgtttt ccttctttca aatccggctg cgccctttgg gtcgcggccg gcccctaaat
     3541 ggcgttcgcg gctgtcgctt caatgaatgg atcgttcaga atatgacaat tttcttacct
     3601 gtgttatttt taccacacgg tatggaatag ctatgcgaaa tcgaatccgc ggaaccgctg
     3661 atttctggct atttttgtga ggaagaaatc ggtcgcgaag accgatcagc cgagcgcggc
     3721 ctgtttttcc tcggcgatgc ggtcgagttc ggcgcgcgtc ggcttggttg ccgcgctctt
     3781 cagctgtccg catgccgcca tgatgtcgcg cccgcgcgga gtgcgcaccg gcgccgaaat
     3841 gccggccttg aaaatgaggt tgctgaacgc gcgcacccgc tcgggggtcg aacattcata
     3901 gggcgcgccg ggccaagggt tgaaggggat caggttgacc ttggcgggca gattatattg
     3961 cttgatcagc cggacgagtt cgcgggcgtc cgcgtcgctg tcattcttgt ccttcagcat
     4021 cacatattcg aaggtgatgc gccgcgcatt gttcgcgccg ggataatcgg cgcacgcctg
     4081 cagcagctcc tcgatgccat atttgcggtt caggggcacg atctcgtcgc ggatttcctt
     4141 gttcaccgcg tggagcgaga ccgcgagatt gaccccgatc tcttcgcccg cgcgcgccat
     4201 catcggcacg acgccgctcg tcgacagcgt gatgcggcgc ttcgacagcg cgagtccgtc
     4261 gccgtccatc acgatcttga gcgcgccctt gacctcgtcg aaattataga gcggctcgcc
     4321 catgcccatc atcacgatgt tggtgagcat gcggccgtcg gcggtatagt gaccagcggc
     4381 atcctcgtcg gcatcgtcat cttcgggatc ggcgccgaag cccgccatcg tgcctttggg
     4441 ccattcgccg agcgcgtcac gcgcgagcag cacttgcccg acgatctcgc ccgcgccgag
     4501 gttgcgcacg aggcgcattg tgcccgtgtg gcagaaacgg cagttcaatg tgcagccgac
     4561 ctggctcgat acgcagagcg ttccccgatc ggcgtcgggg atgaagacca tctcatattc
     4621 ctgcccgtcg gcggcggcga gcagccattt gcgcgtgccg tcgttcgaca cctgcgcctc
     4681 gcgcaccgtc gggcggccga tgatgaagcg gtcggtcagc cacgggcgca tcgtcttggc
     4741 gatgtcggtc atcgcttcga aatcggtgac gccgcgatga tagatccagt gccagatctg
     4801 cttggcgcgc agcttcgccg ccttcgcgtc gaggcccgcc tcgacgagca cgccgccgat
     4861 cgcgtcgcgc gtcagcccga cgaggtcgat gcggttgccg ccgcgcagcg ggacggtgcc
     4921 cgtcgtgacg ggatcgatat ggccgggaat ctgcatgatg cgcggccata tagtgggaga
     4981 ggggcgaaag cgcaattggg cgcgactatg ccctctcccc tttaggggag aggatatgaa
     5041 ggcttggcaa cttgttgcct agccggagtt ggagaggggc gggcctcgat cttgcccctc
     5101 tccaagcttc gctagctcct gacggagcaa gcttcgctat cctctcccct caaggggaga
     5161 gggctgatgg attacagcga cttcagcatc gccagcatct gcggcacggt gccgttcgcc
     5221 tcggcggtgc ggagcggcag gacgttctgc acgaggattt cctcgggcgt cggctgctgc
     5281 gcgaacagcg ccatcacctc gtcggcaaag tcgtcgagcg gcatataatg ctcgcgcgtc
     5341 gactgaccgg gggtcaattc ggtgcgcacc gcgggcggag cgagttcgat cacctcgacc
     5401 tggccctcga gctgggcgcg cagcgcgaca gaatagctgt gcatcgccgc cttggtcgcc
     5461 gaataggtcg gcgccttggg gaagggcacg aaggcgagcc ccgacgttaa gttgacgatc
     5521 gcgctgtccg gcctcgccgc gagatggtcg acgagcgcgt cgatcagccg gatcgggccg
     5581 aggatgttgg tgacgacggt cgcttcggca tgcgccaggt cgcgcttcgc gctcgcatcc
     5641 tcggcgctca tgatgccggc attgttgacg aggatgttga ggtcgggatg cttcgcgacg
     5701 atctccttcg cgaacaccgc gatcgcgtcg gcatcggtga cgtcgagcga tatcgccgac
     5761 atattgggac gtccggcgat cgccgcgtcg agtttggcgg cgttgcggcc ggtgacgatg
     5821 acatggttgc ccgcgtcgtg ccagcgctgc gcgagcgcga gcccgatgcc cgaaccgccg
     5881 ccggtgacga ggatggtgtt gccgctggtc ttcatggcgc ttctcctttg gggggtggtg
     5941 agaaggccta aatattccta tactctccaa aagaaagaag gcacccgaaa gtgccatagt
     6001 tacccgaaag agagttttgg attttccgct atgcccgcaa ccgaaaaaat ttcctacgac
     6061 ccgaacgcgc cggtcgatcc gcgcgtcgaa acactcgtca acgaactcat tggccgagtc
     6121 gccgacaaat ggactctcct cgttctcgag gagctggaag accatggcat ctgccgcttc
     6181 acggagctgt cgcggctcgt tcccgggatc agccagaaga tgctgaccca gaccttacgc
     6241 gcgatggagc gcgacgggtt gctcgtgcgg acggtacacc cggtggtccc gcccaaggtc
     6301 gaatattgcc tgaccgatct cggccatagt ctgggcgaag ccttttgcgg ggtctggatc
     6361 tgggccgaaa ccaatctcga ccggatcgaa aaggcgcgat cggccttcga cgcgcgcggg
     6421 tagcgcctta ccgtttccgc ccgaattgcc ccggcaggct ggtggcgcgg gtcaggccgg
     6481 ggcgccaggt gtcgacacgc ggcgggggga tgatctgaaa atcggccctg ctgtcggcgg
     6541 cgacgatctg gtcggtgtgc gcggcgcgca gccgcatggt ttcgatctcg cggtcggtct
     6601 ggatgccaaa gcgtgtgccc gcgacccagc gcaccgtccc cagcatcggt tcgtgaccgg
     6661 gcatgaacac cgtcatgcgc tcaccgatct gcaggatatg ggggccctga ccgccgattc
     6721 cggttagcga gacgttgcga agcgtgacgt cgaaccggcc cagccgctgg caggcgagcg
     6781 ccgccttgac cagccgcgac tgccgcggct ggcgcttttc ctccggggtt gatgaggcag
     6841 ggaaggcctc gggattcgac atgcgcgact cagtatttgc cgcccgattc ccacctcgga
     6901 cggttatcct tttggtcccg ggcgccagtc ctaaacaggg aaggtaaatt ttcgtatatc
     6961 gtgtttgata attatgcgga attatctcgc cgtgggcgcg cctcaataag ccagcgcaca
     7021 tccgtcggcg cgcatctcgc tcgccgcggc atagacgcgc gtgtcgccgt ccatgcgatg
     7081 ctcgacgcat tgatagcggc cgaacccgcc atcgggttcg cccagcgacc agccgatatc
     7141 ggcgagccgc cgccgggtcg cctcgggcac gccgctttcg aggcgcagca tgccgttcgg
     7201 gccgaggccg ggcgaatcct cgcccatcga ttgcgacgat ccctcatggt gccagcgcgg
     7261 cgaatcgccg gccgactgga tttcgagccc gtaatcgacg cggttgatga tgatctgcgc
     7321 ttgtccctgc ggctgcatgt cgccgcccat cacgccgaag ctcatccaag gcacctgccc
     7381 gcgcgccgcg aagcccggga tgatcgtctg gaacggccgc ttgcccggcg catagatgtt
     7441 cggatgcccg tcctgcagac tgaacaattg cccgcgatcc tggaacatga agccgaggcc
     7501 atcggcgacc agccccgaac ccatgccgcg gaagttcgac tggatcatcg acaccatcat
     7561 cccgtccttg tcggcgcagc tgaaataggt ggtgtcgccg tggctcggtg cctgtccggg
     7621 atggaccggc gacagcaggc ggtcggggcg gatcagcttg gcgcgttcgg ccgcatattc
     7681 ctttgaaatc agccattcga ccggcacgtt cgaaaaatgc ggatcggcgt agtaacgcgc
     7741 gcgatcctca taggcgagac gcttcgcctc ggcctgcagg tgaatcgaca gcgccgactg
     7801 gaagcccgcg cccttgaggt cgaaatgctc gaggatattg agcatttgca gggtcgcgat
     7861 cccctgcgtg ttcgcgccga gcgcatagac gtcggtgccg cgatagccgg tcttgtgcgg
     7921 ttcgatccat tcggacttgt gcgcggcgag atcctcgtag cgcagccagc cgccgatccg
     7981 cttgaaatag gcgtcgatcg tgcgcgcgat ttcgccctcg taaaagacgt cgcggccgcc
     8041 ttcggcgatc agacggaagg tgcgcgcgag atcggggttg cggaaaacct ggccggccgc
     8101 gggccccttg ccgtcgttca ggccgtaggt tcggatcgca ttgtcgacct cttcgatccc
     8161 gcggctaggc tggcggaaac cggcaaggct gcggcggata taataagcga tcatgtcggg
     8221 caccggcgcg cccgcctcgg catgggcgat gaccggctcg aacagctcct tccacggcag
     8281 cttgccgtaa cgctggtgca tcgtccacca gccgtcgacc gtgcccggca ccgacacgct
     8341 gatcgcgcca taggcgggca gggtgccgcc ctttgcacgg ctgcgcaccg tcgcgaggtc
     8401 gagcccgcgc gggctcttcc ccgacccggc gagccccgcg agcttc
//
LOCUS       DATMSU010000161         4158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_116735,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000161 DATMSU010000000
VERSION     DATMSU010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116735"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..1280)
                     /locus_tag="VN034_04220"
     CDS             complement(381..1280)
                     /locus_tag="VN034_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWV59835.1"
                     /translation="MRDLSMFVVLAAFALSGAAAAKEPLRLKPSSKWNVHYADDSCRM
                     GRSFGTGDQKVVLVADRYQPGGDLRLSFVGKPVHTRVDEGVVRIRFGPAEAEQEVRFF
                     PGLLADKTPTLILQRGIGIAPYPPEVLKAYEAAIEAEKYDFEWPEITPEREAAVTYIE
                     VKRSVRQPFILETGSLGAPLAALEKCTDELLGHWGIDVARHASLSRPVRTKSNPGNWV
                     KSRDYPDDMLRAGKRAIVHFRLNVDAAGRPTACHIQQSTRPKAFDDAVCKAIMRNAEF
                     DPALDAEGNPIASYWLNSVVFHI"
     gene            complement(1385..2527)
                     /locus_tag="VN034_04225"
     CDS             complement(1385..2527)
                     /locus_tag="VN034_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWV59836.1"
                     /translation="MKRLIDMLMGRERVLINENERAIWLYKGEVRGILGAGEHVLTNR
                     DGSLRLEIMPLTQMLFKSAYEQAVFEKLPVDAATHLTVVRTAENEIAVIERDGIINTV
                     LGPDAKLILWTDAGPWKAVTVDIADSPRVEAKLLQRLQKAGKAQLVTMVEVDEGKTAL
                     VSIDGRMAERLEAGVTGYWNPGRKIAAKLVDLTRQALDVTGQEVLTRDRVTLRINISA
                     EYRVVDPLLAVTSVKDFADALYRALQLAFRKTLGALTLDQILEKKVTVDAEAANKVRA
                     DMAAIGVEVSDIALKDVVLPGEMREILNRVVAAQKEAEANVIRRREETNATRSLLNTA
                     KVMAENPVMLRLKELEALESIAGKVERLTVTNGTEGLLNDLVRLRD"
     gene            complement(2909..2979)
                     /locus_tag="VN034_04230"
     tRNA            complement(2909..2979)
                     /locus_tag="VN034_04230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2945..2947),aa:Leu,seq:tag)
     gene            complement(2978..3052)
                     /locus_tag="VN034_04235"
     tRNA            complement(2978..3052)
                     /locus_tag="VN034_04235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3018..3020),aa:Arg,seq:tcg)
     gene            complement(3242..>4158)
                     /locus_tag="VN034_04240"
     CDS             complement(3242..>4158)
                     /locus_tag="VN034_04240"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HWV59837.1"
                     /translation="LVGAGISAAAAKYEVDRLAKDPMAAMLRRQAGRMAKQDWLLANQ
                     ERLAREAEGGFALDTLADPEPASFYRHHYEANRPAKLNGIIDHWPALARWSLDHFARA
                     AGDAVVEAQVERERDPDYELAKDGHRRLIRFAELIDWLRKDEASNDIYLTAYNSGTNA
                     AALTPLWDDMAPIAILDDTRTRDGFFWLGPKGTLTPWHHDLTNNLLVQVLGRKRVRMA
                     PPWAFARMRNSRHCFSDWGNEALPAGDGDAATPPVLETIIGPGEAVFLPVGWWHQVEA
                     LDLSASMSFTSFRRPNSHVEDYQSWGAF"
BASE COUNT          760 a         1410 c         1260 g          728 t
ORIGIN      
        1 gcgagcacgc ccacgacctc gggcgcccga acccccgccg caaacgccgc ccgcaccagc
       61 gccgcctcgt cgccatgacc gaacggacga tccgccatat acgccgccga cggcgcacga
      121 cgcagaacat aacccgccgc cgccccaccc gacccccagt cgaacgacca gctctccata
      181 ttcgccccgc cagacaggcg aacgagtccc gataaggaac cgccgcccgc aacccgggtc
      241 atgaaaccag acaaagcatc gcaaaggtcg gtcatgcgcc gatactgctt gcagcttacg
      301 taaacggcaa gccaccaatt gcgtcagggc ggcggcggct caggaaagcg aagggtccgg
      361 gcggctgccc aaacgacggc tcagatatgg aacacgaccg aattcaacca ataggacgcg
      421 atcggattcc cctccgcatc gagcgcgggg tcgaattcgg cgttgcgcat gatcgccttg
      481 cacaccgcgt cgtcgaacgc cttggggcgc gtcgactgct ggatatgaca cgccgtgggc
      541 cggcccgcgg catcgacgtt caaccggaaa tgcacgatgg cgcgctttcc cgcccgcagc
      601 atatcgtccg gataatcacg cgatttcacc caattgccgg gattgctctt cgttctgacc
      661 ggccgtgaca aggatgcatg tctggcgacg tcgatccccc aatggccgag caattcgtcg
      721 gtacattttt cgagcgccgc gagcggtgcg cccagcgatc cggtttcgag gatgaacggc
      781 tgacgaaccg atcgtttcac ttcgatatag gtgaccgcag cttcgcgttc gggcgtaatt
      841 tcaggccatt cgaagtcgta tttctccgcc tcgatcgccg cttcataagc cttcaggacc
      901 tcgggcggat agggcgcgat gccgatgccg cgctgaagga tcagcgtcgg tgtcttgtcg
      961 gcgagcaaac cgggaaagaa tcgaacctcc tgctcggctt cggccggtcc gaacctgatc
     1021 cgcaccacgc cttcgtccac gcgggtatgt acgggcttac cgacgaaact cagccgcaga
     1081 tcgccgcctg gctgatagcg gtcggcgacc agcacgactt tctgatcgcc cgtgccaaag
     1141 ctgcgcccca tacggcagct gtcgtcggca taatggacgt tccatttcga gcttggtttc
     1201 agccgcagcg gctccttggc cgcagcagcg ccagacagcg caaacgccgc cagcacaacg
     1261 aacatcgaca gatcgcgcac cattcccccc atcggacgca gccttgccga ccaaaacgcg
     1321 aaacgcaagt cacgatgaag aaaggggccg gctttcaccg gcccctccct ttgtggcgca
     1381 aggctcagtc gcgcaggcgc acgaggtcgt tgagcaggcc ttcggtgccg ttcgtcaccg
     1441 tcagccgctc gaccttgccc gcgatgcttt cgagcgcctc gagctccttc aggcggagca
     1501 tgaccgggtt ttcggccatg accttcgccg tgttgagcag cgagcgggtg gcgttcgttt
     1561 cctcacgccg gcggatgacg ttcgcctccg cctccttctg cgccgcgacc acccggttca
     1621 ggatctcgcg catctcgccc ggcaggacca cgtccttgag cgcgatgtcc gagacctcga
     1681 ccccgatcgc cgccatgtcg gcacggacct tgttcgccgc ctcggcatcg accgtcacct
     1741 tcttttcgag gatctggtcg agcgtcagcg cgccgagcgt cttgcgaaag gccagctgca
     1801 gcgcccggta gagcgcatcg gcgaaatcct tcacgctcgt gaccgcgagc agcggatcga
     1861 cgacgcgata ttccgcgctg atgttgatcc gcagcgtcac gcggtcgcgg gtcagcactt
     1921 cctgccccgt gacgtcgagc gcctggcggg tcaggtcgac cagcttcgcg gcgatcttcc
     1981 ggcccgggtt ccagtagccg gtgacgcccg cctcgagccg ttcggccatc cggccgtcga
     2041 tgctgacgag cgcggtcttg ccctcatcga cctcgaccat cgtgacgagc tgcgccttgc
     2101 ccgccttttg cagacgctgg agcagcttcg cctcgacccg cgggctatcc gcgatgtcga
     2161 cggtcaccgc cttccacgga cccgcgtcgg tccacaggat cagcttggcg tccggaccga
     2221 gaacggtgtt gatgattcca tcgcgttcga tgacagcaat ctcgttctcg gcggtgcgaa
     2281 ccacggtcag atgcgttgcc gcatcgaccg gcagcttttc gaacaccgcc tgctcataag
     2341 ccgacttgaa cagcatctgc gtgagcggca tgatttcgag gcgcagcgaa ccgtcgcggt
     2401 tcgtgagcac atgctcgccg gcacccagga tgccgcggac ctcgccctta tagagccaga
     2461 tcgcccgttc gttctcgttg atgagcacgc gttcgcgccc catcagcata tcgatcaatc
     2521 gcttcatcat ctcattcacc attctcaaat ctgcgtcacg tcacgccgat ttcctttctt
     2581 cgttggagtt catcgatttc cccgagccat aggcgcggag gctggcggca tggcggcgag
     2641 cgggcagcgc gcgagggggc gtcgcggcgt ccggtgcggg gcgatggggt tcgaaaaccc
     2701 ccgagcccga catcggcgca gcgcagccat catcgcctgc caagccttcc ggcatggctt
     2761 cggaacggaa gccggtccca cccgtgcccg agggcaagga gcgagatcgg cgccagtctt
     2821 cggcaagcca gcccccgaac cattaattgc aaagacagcg tttcaggctg ttacagctta
     2881 agaggctgtt ggtcgtgcaa aggaccggtt ttggtgcagg attcgaaccc gcgacaccaa
     2941 ggacctaaac cttgtgctct cccactgagc taaccaatgg cacagacgac aggattcgaa
     3001 cccgcaacct ccggtcccga agaccggcgc tctcccattg agctacgtcc gcgaacctga
     3061 ccaaacgcgg cggtatccgt acagcgaacc ggcagcgccg gtcgttgcgg gcgggcttcg
     3121 cgaaaccccg atccgttgac atccgccccg ccgactggcc ggggacggcg ccgttagacg
     3181 cgacccaaat agagcgcaag aagaaaaatc acatataaag aagaatttat atcgggttcg
     3241 actaaaacgc accccatgac tgatagtctt cgacatggct attgggccgg cgaaagctgg
     3301 tgaaactcat gctcgccgac aggtcgagcg cctcgacttg atgccaccag ccgacgggca
     3361 gaaagacggc ttcgcccggc ccgatgattg tttcgagcac gggcggcgtt gcggcatcgc
     3421 cgtcgccggc gggcagggct tcattgcccc agtcggagaa gcaatggcgg ctgttgcgca
     3481 tccgcgcgaa cgcccagggc ggcgccatgc gcacgcgctt gcggcccagc acctggacga
     3541 gcagattgtt ggtgaggtcg tgatgccagg gggtgagcgt gcccttcggc cccagccaga
     3601 aaaagccgtc gcgcgtccgg gtgtcgtcga ggatcgcgat cggcgccata tcgtcccata
     3661 gcggggtcag cgccgcggcg ttggtgccgc tgttataggc ggtgagatag atgtcgttgc
     3721 tcgcctcgtc cttgcgcagc cagtcgatca gttccgcgaa acggatcagg cggcgatgac
     3781 cgtccttcgc gagttcatag tcgggatcgc gctcgcgctc gacctgcgcc tcgacgacgg
     3841 cgtcgcccgc ggcgcgcgcg aaatggtcga gcgaccagcg cgcgagcgcg ggccaatgat
     3901 cgatgatgcc attgagcttc gcgggccggt tcgcctcata atggtgacga tagaaggacg
     3961 cgggctcggg atcggccagc gtgtcgagcg cgaaaccgcc ctcggcctcg cgcgcgagcc
     4021 gttcctgatt ggcgagcagc caatcttgct tcgccatccg ccccgcctgc cgccgcagca
     4081 tggccgccat cggatccttg gcgagccggt cgacctcata tttcgccgcc gccgccgaga
     4141 tgcccgcgcc gacgaggc
//
LOCUS       DATMSU010000162         1938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_69270, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000162 DATMSU010000000
VERSION     DATMSU010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69270"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /gene="aroA"
                     /locus_tag="VN034_04245"
     CDS             <1..1002
                     /gene="aroA"
                     /locus_tag="VN034_04245"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWV59838.1"
                     /translation="SHPITTTFIGDASLSGRPMGRVIDPLSEMGADISASPGGRLPLM
                     VRGLAPAIPLSYRLPMASAQVKSAVLLAGLNTPSITEVIEPVPTRDHSERMLRGFGAE
                     LTIEVDGEGTRHIRIRGEAELKPQTIIVPGDPSSAAFFIVAALVVPGSDVTIANVGLN
                     PTRAGLVEVLQQMGGDITLLDAREVGGEPVADLRVRHSNLKGIAVDPAVAPSMIDEFP
                     ILFVAAALAGGRTVTTGLDELRVKESDRLAVMAAGLKAIGARVEENEDGLVIDGTGGD
                     PLPGGATIAGHLDHRICMSFAVAGLVSKAPVTVDDIAPVATSFPNFETLLEQLQQGL"
     gene            1079..1609
                     /locus_tag="VN034_04250"
     CDS             1079..1609
                     /locus_tag="VN034_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59839.1"
                     /translation="MKIQTAAIAALLLAYSGGAANAGERIADPFDRADASFSRYPLNL
                     PTTPQAIGRLTNGDVCGGEEGIDCEWEDESGVRHVVVGDDIIAIKTVDVAALGNRHIA
                     ALDIGTARARGDVLAKVCAFLPEIAIDCLEPGKAGEGKGIASCGGSFDNGGWIKLLFG
                     PAGRLTSARIDAFQIN"
     gene            1609..>1938
                     /locus_tag="VN034_04255"
     CDS             1609..>1938
                     /locus_tag="VN034_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWV59840.1"
                     /translation="MIIAVDGPTASGKGTIARALAAHFGLPVLDTGLLYRAVGYQTQL
                     NHGDPDNAADALAACDFPDSLLDDPALRFESTGSLASRASVHPAVRAALLQRQVDFAN
                     QPGGAVLD"
BASE COUNT          313 a          694 c          629 g          302 t
ORIGIN      
        1 agccacccga tcaccacgac cttcatcggc gatgccagcc tgtcgggccg cccgatgggc
       61 cgcgtcatag atccgctgtc agagatgggt gccgacatca gcgcctcgcc cggcggccgc
      121 ctgccgctga tggtccgcgg cctcgccccc gccatccccc tttcctaccg cctgccgatg
      181 gcgtcggcgc aggtgaagag cgcggtactg ctcgcggggc tcaacacgcc gagcatcacc
      241 gaggtgatcg agcccgtccc gacccgcgac catagcgagc gcatgctgcg cggcttcggc
      301 gccgagctga ccatcgaagt cgatggcgaa ggcacgcgcc atatccgcat tcgcggcgaa
      361 gccgaattga agccgcagac gatcatcgtc cccggcgacc cctcgtccgc cgccttcttc
      421 atcgtcgcgg cgctggtcgt gcccggatcg gacgtcacca tcgccaatgt cggcctcaac
      481 ccgacgcgcg cgggcctcgt cgaggtattg caacaaatgg gcggcgacat cacccttctc
      541 gatgcgcgcg aagtcggcgg cgagcccgtc gccgacctgc gcgtccgcca cagcaacctc
      601 aagggcatcg cggtagaccc cgccgtcgcg ccgagcatga tcgacgaatt cccgatcctg
      661 ttcgtcgccg ccgcgctcgc cgggggccgc acggtgacga ccgggctcga cgagctgcgc
      721 gtcaaggaaa gcgaccgcct cgccgtcatg gcggccggcc tgaaagccat cggcgcgcgc
      781 gtcgaggaaa acgaggacgg cctcgtcatc gacggcaccg gcggcgaccc gctgcccggc
      841 ggcgcgacga tcgccggcca tctcgatcac cgcatttgca tgagtttcgc ggtcgcgggc
      901 ctcgtcagca aagcgccggt gacggtcgac gacatcgcgc cggttgcgac cagctttccc
      961 aatttcgaaa cgctgctgga gcaattgcag cagggcctgt gattgccgtc atcgacgacg
     1021 accgtggttc gtggcaggat tgcggctcga tttcaggata ttaagggatt tgcgcacgat
     1081 gaagatccag accgctgcca tcgccgccct gctccttgcc tattcggggg gagccgcgaa
     1141 tgccggcgag cgcatcgccg atccgttcga ccgcgccgac gcgagcttct cgcgctatcc
     1201 gctcaattta ccgacgacgc cgcaggcgat cgggcgactc accaacggcg acgtgtgcgg
     1261 cggcgaggaa ggcatcgatt gcgaatggga agatgagtcg ggcgtgcggc atgtcgtcgt
     1321 cggcgatgac atcatcgcga tcaagacggt cgacgtggcg gcgctcggca accgccatat
     1381 cgccgcgctg gatatcggca ccgcccgcgc gcgcggcgac gtgctggcca aggtctgcgc
     1441 cttccttccc gaaatcgcaa tcgactgtct cgaacccggc aaggccggcg aaggcaaggg
     1501 catcgcgtcg tgcggcggca gcttcgacaa tggcggctgg atcaaattgc tgttcggtcc
     1561 cgcgggccgc ctgacgagcg cgcgcatcga cgcgttccag atcaactgat gatcatcgcg
     1621 gtcgacggtc ccaccgcgtc gggcaagggc acgatcgccc gggcgctcgc cgcgcatttc
     1681 ggcctgcccg tgctcgacac cgggctgctc tatcgcgcgg tcggctatca gacccagctc
     1741 aaccacggcg accccgacaa tgccgccgac gcgctcgccg cgtgtgactt tcccgacagc
     1801 ctgctggacg atccggcgct gcgcttcgaa agcacgggca gtctcgcgag ccgcgcgtcg
     1861 gtccatcccg cggtgcgcgc cgcgctgctc cagcggcagg tcgatttcgc gaaccagccc
     1921 ggcggcgcgg tgctcgac
//
LOCUS       DATMSU010000163         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_135411,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000163 DATMSU010000000
VERSION     DATMSU010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135411"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2530)
                     /locus_tag="VN034_04260"
     CDS             complement(<1..2530)
                     /locus_tag="VN034_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HWV59841.1"
                     /translation="MRAAGWITMAMAALAAPQALAAEAGSQASLDRLADTLGYRLTMV
                     DNGPACPAGIEGCFLTTITLTVPENLPADMPTKGLALYFSFVNRLPLVESDIFAHRLI
                     NGDVQQLTLKPGATLRPGAKHEIKLWGVGSNFSKAFGMPNAYLVADGLDPRTIAATRP
                     VIDPETGLETLPFVAPMTDEAKLATKSDADKTRWLTAERAFDLQATRLAPEATDTVIL
                     PKPAKAVQGKGQPVELGRGVALTLSGVERSAIAPALAALGAPEGALPLTIRIDRAAAA
                     KPESYILDAKASGIAITAHDAAGASHALRSLAQQAAFEKGKLTPLHVEDAPEYGFRGL
                     HIDLGRNFHSRDEILKLVEAMAAYKLNKLHLHLADDEGWRIEIPALPELAEIGSKRCH
                     DPSERTCILPQLGAGPDGTGSVNGYLSTADYVAIVEAAAARQIEVIPSIDMPGHSRAA
                     IKAMEVRHARLTAAGKQAEADEYRLIDPADTTEYRSIQNYDDNTLNVCLPATYRFVDT
                     IVDALAAMHKQAGVPLKTFHLGADETAGAWVKSPACKTMIAENGGDAGKLTPRFIERV
                     TASLAAKGLRTGGWSDGMGHTETAVMPKQPHLVQTNIWGVLHTGAIREAHDQMNRGWD
                     AVLSIPDLGYFDMPYAPHPDEGGYDWASRGVDPYQVFGFMPDNLPANAATIRNIFAEG
                     KPIEDKPVLQPGHRVAGLQAQLWSETIRTDAQVDYMLFPRLLALAERAWAPAPWTPPY
                     RAGVSYEWGDSRVDAAKLKAGWQDFAGRVAAQWPMLERIGVAYRVAPPGARIANGKLE
                     ANSMFPGTVIEYRVGDGAWTRYAGPVAVGGAAELRSRSADGKRAS"
     gene            2631..3674
                     /locus_tag="VN034_04265"
     CDS             2631..3674
                     /locus_tag="VN034_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HWV59842.1"
                     /translation="MTNIRAFRLIASILIGFTLVLSLPVRAEERRPVVVGYLAAFKGL
                     DLSIARTDLAAFTHFNLSFANPDAAGRFVDNGKMTCMGDETGANLSVEALRGTVARLQ
                     ASGGKVLISTAGGVIPGCSGDWKALLAPERRAATVAALVELADTLGLDGVDIDIEGKL
                     LTEIDRAGDYTPFIAALSEALKARGKLLTCATASYEGGMIPVSSIRYFDLVNVMSYDA
                     IGPSWGEAGAEHSSYAMAARDLDLWRARGVARERLVLGVPFYGYGFGGEKANWSYRDL
                     AAAHGLNATKADVIGERCAGCRYITHNSPATIETKARLAAEKAGGVMVWELSQDSPDH
                     ALTKAIGRGLSRE"
     gene            3774..4511
                     /locus_tag="VN034_04270"
     CDS             3774..4511
                     /locus_tag="VN034_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWV59843.1"
                     /translation="MSLIERVGPLQKDDPTPLYLQLQKILRDAIEGQVVKAEEAIPTE
                     RDLAEEFDVSRITVRKAIDGLVADGIVTRRRGAGTFVTRPRVEKSFSKLTSFSEDMIS
                     RGRKPHSKWVSKTAGAVTPEEALSLGLSPGSLVYRFHRIRYADDTSMALEYATIPAYC
                     LPSVEAVGASLYEALEAAGHLPVRALQRLRAIAATPEQADMLEIEPGTPGLFIERRGF
                     LSDGRTAEFTQSFYRGDAYDVVAELNG"
     gene            complement(4601..>6041)
                     /locus_tag="VN034_04275"
     CDS             complement(4601..>6041)
                     /locus_tag="VN034_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HWV59844.1"
                     /translation="SAGWMTAAALGRALAGQSHSITLVESEAIGTVGVGEATVPPIHE
                     FNRYLRLDQAEFLRATHGTIKLGIEFEGWGGAGHRYFHPFGYLGIEMSGIHFHHHWLR
                     HLAAGGDADHGPYNLETMAAREGRFAPATRHGPVHHAVHFDAAAYARFLRSYAEDKGV
                     ARIEGKVADVRLDPEDGFITALALDDGRTVEGDLFVDCSGFRGLLIEGALHTGYTEWS
                     HWLPCDSAVAMPCESEGDPAPFTRATAREAGWQWRIPLQHRTGNGYVYSSAHLGHDEA
                     EALLRARLPGAPLADANRLRFTAGHRKAIWNRNCVAIGLAGGFLEPLESTSIHLIQTT
                     IVRLLALLPRDAIDPHVVRRFNRDALHEYKVIRDFVIAHYALANRDDTPFWRDVAAAG
                     LPDSLAERIEAFRATGNILFEPGDLFGPTNWYAVLTGQGVRPQSWHPIADGLSDAELA
                     ARLATLRGRVAEQLAELPPHAEFLRRLDS"
BASE COUNT          957 a         1885 c         2149 g         1050 t
ORIGIN      
        1 ggctggcgcg cttgccgtcg gcggagcggc tgcgcagctc ggccgcgcca ccgaccgcca
       61 ccggtccggc atagcgcgtc catgcgccat cgccgacacg atattcgatc acggtgccgg
      121 ggaacatgct gttcgcctcg agcttgccgt tcgcgatgcg cgcgccgggc ggcgcgacgc
      181 gataggcgac gccgatccgt tcgagcatcg gccattgcgc cgcgacgcgt ccggcgaaat
      241 cctgccagcc ggccttgagc ttcgcggcgt cgacgcggct gtcgccccac tcatagctga
      301 cgcccgcccg atagggcggg gtccagggcg cgggcgccca ggcgcgctcg gcgagcgcga
      361 gcaggcgcgg gaagagcata tagtcgacct gcgcgtcggt gcggatcgtc tcgctccaca
      421 gctgcgcctg caatcccgcg acgcggtgtc cgggctggag gaccggcttg tcctcgatcg
      481 gcttgccctc ggcaaaaatg ttgcggatcg tcgcggcgtt ggcgggcaga ttgtcgggca
      541 tgaagccgaa gacctgatac ggatcgaccc cgcgcgaggc ccagtcgtag ccgccctcgt
      601 cgggatgcgg ggcatagggc atgtcgaaat agccgaggtc ggggatcgac agcaccgcgt
      661 cccagccgcg gttcatctgg tcgtgcgcct cgcggatcgc gccggtgtgg agcacgcccc
      721 agatgttggt ctgcaccaga tgcggttgtt tgggcatgac cgcggtttcg gtgtgcccca
      781 tgccgtcgct ccagccgccg gtgcgcaggc ctttcgcggc gaggctggcg gtcacgcgct
      841 cgatgaagcg cggggtgagc ttgccggcgt cgccgccgtt ttcggcaatc atcgtcttgc
      901 acgcgggcga tttgacccac gcgcccgcgg tttcgtcggc gccgaggtgg aaggttttga
      961 gcggcacccc cgcctgcttg tgcatcgcgg cgagcgcatc gacgatggtg tcgacgaagc
     1021 gataggtcgc gggcaggcag acgttgagcg tattgtcgtc gtaattctgg atgctgcgat
     1081 attcggtcgt gtcggcgggg tcgatcagac gatattcgtc ggcttcggcc tgtttgcccg
     1141 cggcggtcag gcgcgcgtgg cgcacctcca tcgccttgat cgccgcgcgg ctgtggccgg
     1201 gcatgtcgat cgacgggatg acttcgatct ggcgcgccgc cgcggcctcg acgatcgcga
     1261 cataatcggc ggtcgaaagg tatccgttga cgctgccggt gccgtcgggc ccggcgccga
     1321 gttgcggcag gatgcacgtc cgctcggacg gatcgtggca gcgcttcgag ccgatttcgg
     1381 cgagctcggg aagcgccgga atttcgatgc gccagccctc gtcatcggcg aggtggaggt
     1441 ggagcttgtt gagcttgtac gccgccatcg cctcgacgag cttcagaatt tcgtcgcggc
     1501 tgtggaagtt acgaccgaga tcgatgtgga ggccgcggaa gccatattcg ggggcgtcct
     1561 cgacatggag cggggtcagc ttgccctttt cgaacgccgc ctgctgcgcg agcgagcgca
     1621 gggcatggct cgcgccggcc gcgtcgtgcg cggtgatcgc gatacccgat gccttggcgt
     1681 cgagaatata ggattcgggc ttggccgcgg cggcgcgatc gatccgaatc gtcagcggga
     1741 gcgcgccctc gggagcgccg agcgccgcga gggcgggcgc gatcgcgctt cgttcgacgc
     1801 cgctcaatgt cagcgcaaca ccgcggccga gttcgaccgg ctggcccttg ccttgcaccg
     1861 cctttgcggg tttcgggagg atcacggtgt cggtggcctc gggcgcgagc cgggtggcct
     1921 ggaggtcgaa ggcgcgctcg gcggtgagcc agcgcgtctt atcggcgtcg ctcttggtcg
     1981 caagcttcgc ctcgtcggtc attggcgcga cgaagggcaa ggtttcgagg cccgtttcgg
     2041 ggtcgatcac ggggcgcgtc gcggcgatcg tgcgtggatc gagcccgtcg gcgacgagat
     2101 aggcgttggg catgccgaac gccttcgaga agttcgaccc gacgccccag agcttgatct
     2161 cgtgcttcgc gccggggcgc agcgtcgcgc cgggcttgag cgtcaattgc tgaacgtcgc
     2221 cgttgatcag ccggtgcgcg aagatgtcgc tctcgacgag cggcagccgg ttgacgaagc
     2281 tgaaatagag cgcgagccct ttggtgggca tgtcggcggg cagattttcc ggcacggtca
     2341 gcgtgatcgt ggtgaggaaa cagccttcga tccccgccgg gcacgccggg ccgttgtcaa
     2401 ccatggtgag gcggtagccg agcgtatcgg cgaggcggtc gagcgacgct tgcgatcccg
     2461 cctcggccgc cagcgcctgc ggcgcggcga gcgccgccat tgccatcgtg atccaccccg
     2521 cagcccgcat caattttctc ccgctcgaca atttgtatct aatttgtcat gtagtcgcgg
     2581 gcgggtcaac gcctttcgga tcgaacggaa ttatggggga aattgacaaa atgacgaata
     2641 ttcgcgcttt tcgcctcatc gcgtcgatcc tgattggttt tacattagta ttgtcgctgc
     2701 ccgtccgcgc ggaggagcga cggcccgttg tggtcggcta tctcgcggcg ttcaaggggc
     2761 tcgacctgtc gatcgcgcgc accgacctcg cggccttcac ccatttcaac ctctcctttg
     2821 ccaaccccga tgccgcgggg cgcttcgtcg ataatgggaa gatgacgtgc atgggcgacg
     2881 aaaccggcgc gaatctgtcg gttgaagcgt tgcgcggaac ggtcgcgcgg ctgcaagcga
     2941 gcggcggcaa ggtgctgatc tcgaccgcgg gcggcgtgat ccccggctgt tcgggcgact
     3001 ggaaggcgct gctcgcgccc gaacggcgcg cggcgacggt cgcggcgctg gtcgagcttg
     3061 ccgatacgct cggcctcgac ggggtcgata tcgacatcga gggcaagctg ctcaccgaaa
     3121 tcgaccgggc gggcgactat acgcccttca tcgcggcgct gtccgaagct ttgaaggcgc
     3181 gcggcaagct gctgacctgc gccaccgctt cctatgaggg cgggatgatc ccggtcagtt
     3241 cgatccgcta tttcgacctc gtcaacgtca tgtcctatga cgcgatcggg ccgagctggg
     3301 gcgaagcggg cgccgagcac agcagctatg cgatggcggc gcgcgatctc gacctgtggc
     3361 gcgcacgcgg cgtggcgcgc gagcgactcg tcctcggcgt tcccttttac ggctatggat
     3421 tcggcggcga gaaggcaaat tggtcctatc gcgacctcgc cgccgcacat ggcctaaatg
     3481 cgaccaaagc cgatgtgatc ggcgagcggt gcgcgggatg ccgctatatc acccacaaca
     3541 gccccgcgac gatcgagaca aaagcgcgac ttgccgccga aaaggcgggc ggggtgatgg
     3601 tctgggaatt gtcgcaggac agcccagatc acgcactgac caaagcgatc ggtcgcggcc
     3661 tttcgcgcga ataattcgca acaaggttga cgcgaggata ccaatgcaac acatggggag
     3721 cgggcgctgg tcttatcgat cggcgcgccg ggcaaccaag tggagcatcg tccttgtcac
     3781 tgatcgaacg cgtgggtccg ctccagaagg acgatccgac accgctttac ctgcaactgc
     3841 agaaaatcct gcgcgacgcg atcgaaggac aggtcgtaaa ggccgaggaa gcgatcccga
     3901 ccgaacgcga cttggccgag gaattcgacg tgtcgcgcat caccgtgcgc aaggcgatcg
     3961 acgggctggt cgccgacggc atcgtcacgc gccgccgcgg cgccggcacc ttcgtcacgc
     4021 ggccgcgtgt cgagaagagc ttttcgaagc tgacttcctt ttcggaggat atgatctcgc
     4081 gcgggcgcaa gccgcacagc aaatgggtga gcaagaccgc gggggcagtg acccccgagg
     4141 aggcgctatc gctcggcctg tcgccgggat cgctcgtcta tcgcttccac cgcatccgct
     4201 atgccgacga cacctcgatg gcgctcgaat atgcgaccat ccccgcctat tgcctgccgt
     4261 cggtcgaggc ggtgggggcg tcgctttatg aagcgctgga ggcggcgggg catctgccgg
     4321 ttcgcgcgct gcagcggctg cgcgcgatcg cggcgacgcc ggagcaggcc gacatgctgg
     4381 agatcgagcc gggcacgccg gggctgttca tcgagcgacg cggtttcctt tccgacggac
     4441 gcaccgctga gttcacgcag agcttttatc gcggcgatgc ttatgacgtg gtggcggagc
     4501 tgaacgggta atacttaccg tcattctggc gaaggccgga atctcgacct tgcgtcataa
     4561 tgcaccgatg agatcccggc cttcgccggg atgacgagag ttaggaatcg agccgtcgca
     4621 gaaattccgc atggggcggc aactcggcga gttgctccgc aacgcggccg cgcaacgtcg
     4681 cgaggcgcgc ggcgagttcg gcgtcggaga ggccgtcggc gatcgggtgc cagctttgcg
     4741 gccgcacccc ctgccccgtc agcaccgcat accagttcgt cgggccgaac aggtcgccgg
     4801 gttcgaagag gatgttgccg gtggcgcgga acgcctcgat ccgttcggcg aggctgtcgg
     4861 gcaggcccgc cgcggcgaca tcgcgccaga atggcgtgtc gtcgcggttc gcgagcgcat
     4921 aatgcgcgat cacgaaatcg cggatcactt tatattcgtg caaggcgtcg cggttgaagc
     4981 ggcggacgac atggggatcg atcgcatcgc gcggcaacag cgcgagcagg cggacgatcg
     5041 tcgtctggat cagatggatc gaggtcgatt cgagcggttc gagaaagccg cccgcgagcc
     5101 cgatcgcgac gcaattgcgg ttccagatcg ccttgcgatg ccccgcggtg aaacgcagcc
     5161 ggttggcatc ggcgagcggc gcgccgggga ggcgggcgcg gagcaaagcc tcggcctcgt
     5221 catggccgag atgcgcgctc gaatagacat agccgttgcc ggtgcggtgc tggagcggaa
     5281 tgcgccattg ccagcccgcc tcgcgcgcgg tcgcgcgcgt gaagggcgcg ggatcgccct
     5341 cgctctcgca cggcatcgcg accgcgctgt cgcacggcag ccagtggctc cattcggtgt
     5401 agccggtgtg gagtgcgccc tcgatgagga ggccgcgaaa gcccgagcag tcgacgaaaa
     5461 ggtcgccttc gacggtacgg ccatcgtcga gcgcgagcgc ggtgatgaag ccatcctcgg
     5521 gatcgaggcg gacgtcggcg accttgccct cgatccgcgc gacgcccttg tcctcggcat
     5581 agctgcgcag gaagcgggcg taggcggcgg cgtcgaaatg gaccgcgtga tgcaccgggc
     5641 catggcgcgt cgcgggcgcg aagcggcctt cgcgcgcggc catcgtctcg agattatagg
     5701 ggccgtgatc ggcgtcgccg cctgccgcga gatgcctgag ccaatggtgg tggaaatgga
     5761 tgcccgacat ttcgatgcca agatagccga aggggtggaa atagcggtgg ccggccccgc
     5821 cccacccctc gaattcgatg ccgagcttga tcgtgccgtg cgtcgcgcgg aggaattcgg
     5881 cctgatcgag gcggaggtag cggttgaatt cgtggatcgg cggaacggtc gcttcgccga
     5941 cgccgacggt gccgatcgct tcggattcga cgagggtgat gctgtgcgat tgccccgcga
     6001 gcgcgcggcc gagcgccgcg gcggtcatcc agcccgcgct g
//
LOCUS       DATMSU010000164         4433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_69302, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000164 DATMSU010000000
VERSION     DATMSU010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69302"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VN034_04280"
     CDS             complement(<1..290)
                     /locus_tag="VN034_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HWV59845.1"
                     /translation="MRFIKLIEPQSGLSARCRLLDEAAPESAAFLWDLAERRAGFEAM
                     HAIWTGPEISVPMPASTLPAGMAQPAIPAENATSYPDAGDIVLAYLAAGSTKG"
     gene            complement(299..1972)
                     /locus_tag="VN034_04285"
     CDS             complement(299..1972)
                     /locus_tag="VN034_04285"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HWV59846.1"
                     /translation="MLRRAISLMVGGAALLSAPNAAAQAEVQRLGADFWAWRATTQPA
                     TSDDIPRIVRPQNWTPDWSPVAVAAQQKRLAAFEAEWKALADKPRTVGETVDYRLVGS
                     ALSRVRWELDHVAAWRRQPHFYVAQALNPIFEVLLPPPPLERARIDDVIRLLGNAPAT
                     LAAGKTNLTDMRGPFVDAALHQIAEVPASLEGMVAGIDPHATPAQRRQLKSAKDKAVA
                     AFADFAAFLKAKRTGLSETTAVGRDSYRYFLGSVALYPYSPEELLVMGRQEWARSVLF
                     EELERNRNAGVAELPIGSSIDAVTAKLDADELKVRAFLREKKLLTVPDWAGHYNAQPF
                     PAYLAPIGWLGRTFDLTSQARVGQDATVYLPEPSPKLGYFNLSITRDPRPIIVHEGVP
                     GHFFQLTLGWKHPNPLRRHYYDSGANEGTGFYAEEMMLQAGLWADAPKTREIIYNFAR
                     LRALRVEVDVKLALGKFSIAQAADYLEKMVPMDRATAEEEAVFFAASPGQAISYQIGK
                     MQTAEFLAEARVREGKAFDLKRFHDYLWQNGNVPIALQKEEYLAMVSGK"
     gene            complement(1974..4115)
                     /locus_tag="VN034_04290"
     CDS             complement(1974..4115)
                     /locus_tag="VN034_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWV59847.1"
                     /translation="MPVQGTASAAGTSLSIDELYRYKSLVGTTPEGYAWSADGGTVLF
                     LWNDEGYSFRDIWSYSVKTGKKVRLTTLGQDSKPEAEAKGIAQAVALDRGRIAFTLGG
                     HLHIREANGTIAKVETDKQAIRKLAVSPDGTRLAFISGNPVDTRNRVTLGGVLWVRDL
                     AAKDANAARRVAGDADPKVYLEDFQWAGDGKAIAFQQSDDRAMPERDIFYNAKGELQN
                     NRVIRAFPGDETTKATVGVVTLASGETRFYDRPDAKHHVWDYGLSSDGKRLFVSGSDM
                     EAKEHIIYVYDVASGARETFYQLREPKHLRPDWRVAWAPGDDGLIIMTDRDGWLHLYH
                     QRTAGGTPRQITSGKWEVESFTVDREAGQLYFTANESRLADRQIYRVPVAGGKMQRIS
                     AAAAGTHQPVYSPDFRHIADWYSNDTTPPELYLVDTAKPGRATQVTRSPQPEFYQQTW
                     ADIGYAEFPSHVDGTNLLARISLPANYDPAKRYPVIVGSVYSDAVRNQWGGRRAHPTW
                     GIDQYFVAQGYIVLNVNIRGSWGQGRDHNQTQYHSYGETDINDLESGVRYLVDKGYAD
                     PKRVGIWGSSYGGLMTIMSLSKKPGVYAAGIAGAPATNVWHAYPSQMWIMGPPEGADM
                     PARYEAQSALYQAKGIRDPLMIIHGTRDPVVLYSDTIALAEQMIQREQMFELVTLPGG
                     NHAWASDNLPQARFAFKKMVDFFDRNVKNRK"
BASE COUNT          739 a         1483 c         1388 g          823 t
ORIGIN      
        1 cccttggtgc tgcccgccgc cagataggcg agcacgatgt cgcccgcgtc gggataggat
       61 gtggcgttct cggccgggat cgcgggctgc gccatcccgg ccggcaacgt gctggccggc
      121 atcgggacgg agatttccgg accggtccag atcgcatgca tcgcctcgaa ccccgcgcgg
      181 cgttcggcga gatcccacag gaaagccgcc gattcgggag ccgcctcgtc gagcagccga
      241 caacgtgccg aaagtcccga ttgcggctcg atcagtttga tgaaacgcat gccgccccct
      301 atttcccgct caccatcgcc agatactcct ctttctggag cgcgatggga acattgccgt
      361 tctgccagag ataatcgtga aaacgcttga gatcgaacgc cttgccttcg cgaacgcgcg
      421 cctcggcgag gaattccgcc gtctgcatct tcccgatctg gtaactgatc gcctgccccg
      481 gcgacgcggc gaagaagacc gcctcttcct cggccgtcgc gcggtccatc gggaccatct
      541 tttccagata gtcggccgcc tgggcgatac tgaactttcc gagcgcgagc ttgacgtcga
      601 cctcgacgcg cagcgcgcgt agccgcgcga aattgtagat gatctcgcgc gtcttgggcg
      661 catcggccca cagtcccgcc tgcagcatca tctcttcggc gtagaagccc gtgccctcgt
      721 ttgcgccgct gtcgtaataa tggcggcgta gcgggttcgg gtgtttccag ccgagcgtca
      781 gctggaaaaa atgccccggc acgccctcgt gcacgatgat cggccgcggg tcgcgggtga
      841 tcgacaggtt gaaataaccg agcttcggcg acggttcggg aagatagacg gtcgcatcct
      901 gtccgacccg cgcctggctg gtcaggtcga aggtgcgccc gagccagccg atcggcgcaa
      961 gataggcggg gaacggctgc gcgttataat gccccgccca gtcggggacg gtgagcagct
     1021 tcttttcgcg caggaacgcc cgcaccttga gttcgtcggc gtcgagtttc gcggtcaccg
     1081 cgtcgatcga gctgccgatc ggcaattcgg cgacgcccgc gttgcggttg cgctcgagtt
     1141 cctcgaacag gaccgaacgc gcccattcct gccgccccat cacgagcagt tcctcgggcg
     1201 aataggggta gagcgccacg ctgccaagga aatagcggta ggagtcacgc ccgaccgcag
     1261 tggtttcgga aagcccggtg cgcttcgctt tcagaaaagc ggcgaaatcg gcgaaggcgg
     1321 cgaccgcctt gtccttcgcg ctctttaact gccggcgctg cgcgggggtc gcgtgcggat
     1381 cgattcccgc gaccatgccc tcgaggctgg ccggcacctc ggcaatctgg tgcagcgcgg
     1441 catcgacgaa cgggccgcgc atgtcggtga ggttggtctt gcccgccgcg agggtcgcgg
     1501 gtgcgttgcc gagcagccgg atcacatcgt cgatccgtgc gcgttcgagc ggcggcggcg
     1561 gcagcagcac ctcgaaaatc gggttcagcg cctgcgcgac atagaaatgc ggctgccggc
     1621 gccaggccgc gacatggtcg agttcccatc gcacgcgcga cagcgccgag ccgacgaggc
     1681 ggtaatcgac cgtctctccg accgttcgcg gcttgtccgc cagcgccttc cattccgcct
     1741 cgaacgctgc gagccgcttt tgctgtgccg cgaccgccac aggcgaccag tcgggcgtcc
     1801 agttctgcgg ccggacgata cgcgggatat cgtcgctcgt cgccggctgc gtcgtcgcgc
     1861 gccacgccca aaaatcggca ccgagccgct gcacctcggc ctgcgccgcc gcattcggcg
     1921 ccgaaagcag ggcggcaccg ccgaccatca gggaaatcgc ccggcgcagc atcctatttc
     1981 cggttcttca cgttgcggtc gaagaaatcg accatcttct tgaaggcgaa gcgcgcctgc
     2041 ggaagattgt cgctcgccca agcgtggttg ccgcccggca gcgtcaccag ttcgaacatc
     2101 tgttcgcgct gaatcatctg ctcggcaagc gcgatcgtgt ccgaatagag cacgaccgga
     2161 tcgcgcgtcc cgtggatgat catcagtgga tccctgatcc ccttcgcctg atagagcgcc
     2221 gactgcgctt cgtagcgcgc gggcatgtca gcgccctctg gcgggcccat gatccacatc
     2281 tgcgacggat aggcgtgcca cacgttcgtc gccggggcgc ccgcgattcc cgccgcatag
     2341 acgccgggct tcttcgacag cgacatgatc gtcatcagcc cgccatagct cgacccccag
     2401 atgccgaccc gcttcggatc ggcatagccc ttgtcgacca gatagcgcac cccgctctcg
     2461 aggtcgttga tgtcggtctc gccatagctg tgatattgtg tctggttatg gtcgcgcccc
     2521 tgcccccacg atccgcggat attgacgttt aggacgatat agccctgcgc gacgaaatat
     2581 tgatcgatcc cccatgtcgg gtgcgcgcgc cgcccgcccc attggttgcg caccgcatcc
     2641 gaataaaccg acccgacgat taccggataa cgtttggcgg ggtcgtaatt ggccggcaag
     2701 ctgatacgcg cgagcaggtt cgtcccgtcg acatggctcg ggaattcggc atagccgata
     2761 tctgcccagg tctgctgata gaattcaggc tgcggcgacc tggtcacctg cgttgcccga
     2821 cccggcttcg ccgtatcgac cagataaagt tcgggcggcg tcgtgtcgtt gctgtaccaa
     2881 tcggcgatat gccggaaatc gggcgaatag acgggctggt gcgtgccggc ggcggcggcg
     2941 ctgatccgct gcatctttcc gcccgcgacc ggcacgcgat agatttgccg gtcggccaga
     3001 cgcgattcat tcgcggtgaa atagagctgc ccggcctcgc gatcgaccgt aaagctctcg
     3061 acctcccact tgccgctcgt aatctggcgc ggtgttccgc ccgcggtgcg ctggtgatag
     3121 aggtgcagcc agccgtcgcg gtcggtcatg atgatcagac cgtcgtcgcc cggcgcccat
     3181 gcgaccctcc agtcgggacg cagatgcttg ggttcgcgga gctggtaaaa ggtctcgcgc
     3241 gcgccgctcg ccacgtcata gacgtagatt atgtgctcct tggcctccat gtcggagccg
     3301 ctgacgaaca ggcgcttgcc gtcgctcgaa agaccgtaat cccacacatg atgcttcgca
     3361 tcggggcggt cgtagaaacg cgtttcgccc gatgcgagcg tcacgacgcc gacggtcgcc
     3421 ttggtcgtct cgtcgcccgg aaacgcccgg atgacgcggt tattctgcaa ttcgcccttg
     3481 gcattataga agatgtcgcg ctcgggcatg gcgcgatcgt cggactgctg gaaggcgatc
     3541 gccttgccgt ccccggccca ctggaaatcc tcgagataga ccttgggatc ggcgtcgccc
     3601 gcaacgcggc gcgcggcgtt ggcgtccttt gcagcgaggt cgcgcaccca cagcaccccg
     3661 cccagcgtca cgcggtttcg cgtgtcgacc gggttgcccg aaatgaaggc cagccgggtt
     3721 ccgtcaggcg aaaccgcgag cttgcggatc gcctgcttgt cggtctcgac cttggcgatc
     3781 gtgccattcg cttcgcggat atggagatgg ccgccgagcg tgaaggcgat gcgccccctg
     3841 tccagcgcga ccgcctgcgc gatccccttc gcctcggcct cgggcttgct gtcctgacca
     3901 agcgtcgtca ggcggacctt ctttccggtc ttcaccgaat aggaccagat gtcgcggaag
     3961 ctgtagcctt cgtcgttcca caggaacagc actgtgccgc cgtcggccga ccaagcatag
     4021 ccttcgggcg tcgtgccgac caggctcttg taccgataga gctcgtcgat cgacagcgac
     4081 gttcccgccg cactcgcggt gccttgaacc ggcacgggcc gatcctgcgc cacggcggcc
     4141 ggcgatgcca acgcgagcgc cagcgctgcc ccggccattc ccaataaacc cactttgttc
     4201 atcgacgact attcccagct tcacacgact tcgaacgcga ccggcggagc cgcggcggca
     4261 ccttgccgcc gcggcccaca cctttccact cagaagttcg cccggaaccc gatcgtgaac
     4321 cggcgaccga tcgcgtcgta ggtttcacgt tcgtagaggt agaagggcgg atcgcggtcg
     4381 aacagattgt cgacgttcgc gaagaagctg aaattctccc tcacgtcgta gtt
//
LOCUS       DATMSU010000165         1544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_107494,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000165 DATMSU010000000
VERSION     DATMSU010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107494"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VN034_04295"
     CDS             complement(<1..925)
                     /locus_tag="VN034_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HWV59848.1"
                     /translation="MLIVVGLMVIPVSPLILDISFVANIMISLLILMVALQASKPLDF
                     SAFPTVLLLATLFRLALNVASTRVVLVHGHEGTAAAGHVIEAFGQVLIGGDYIVGLFV
                     FFVLMIINLIVITKGAGRVSEVSARFTLDALPGKQMAIDADLNAGLLTPEEAKARRVE
                     VGTEADFYGSMDGASKFVKGDAVAGLLILFVNIVGGLILGIFSHGLSLSEAGSTYITL
                     AIGDALVAQIPALLLSIAAAAIVTRVSSPFDLSGQIGSQFSSPVIWMAVGGILFILGL
                     VPAMPQMLILPAAALSFAIGWQLRRAQAAVAD"
     gene            complement(988..>1544)
                     /locus_tag="VN034_04300"
     CDS             complement(988..>1544)
                     /locus_tag="VN034_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWV59849.1"
                     /translation="GRLSVADPALRQQIFDLRDDPAASSNMAAALTGDNRAYLESRIG
                     RGAEPVDLYLAHFLGSGGAAKFLTALAANPDQPGASMMPEAAAANRSVFYAADGSMRS
                     LAEIRERFRVKLEEGGKTENMKPFAPSSWHAQASSSSGLASPAGGGRPPLQMMDIQPM
                     PKKLSMGFAADAYRRLASLSGGAA"
BASE COUNT          291 a          467 c          562 g          224 t
ORIGIN      
        1 ggtcggcgac cgcggcttgg gcgcggcgga gctgccagcc gatcgcgaaa gacagcgcgg
       61 cggcgggcag gatcagcatc tgcggcatcg cggggacgag gccgaggatg aagaggatgc
      121 cgccgaccgc catccagatg acgggcgagg agaattggct gccgatctgg ccgctgaggt
      181 cgaagggcga ggagacgcga gtgacgatcg cggcggcggc gatcgagagc aagagggcgg
      241 gaatctgcgc gacgagcgcg tcgccgatcg cgagcgtgat ataggtgctg ccggcttcgg
      301 agaggctgag cccatggctg aagatgccga ggatcagccc gccgacgatg ttgacgaaca
      361 ggatcaagag gcccgcgacc gcgtcgccct tcacgaattt cgaggcgccg tccatcgagc
      421 cgtagaaatc ggcttcggtg ccgacttcga cgcggcgcgc cttcgcttct tcgggggtga
      481 gcagcccggc gttgagatcg gcgtcgatcg ccatctgctt gccgggcagc gcgtcgaggg
      541 tgaagcgcgc cgacacttcg gacacgcggc ccgcgccctt ggtgatcacg atcaggttga
      601 tgatcatcag cacgaaaaag acgaacaggc cgacgatata gtcgccgccg atcagcacct
      661 ggccgaacgc ttcgatgaca tggcccgccg ccgcggtgcc ttcgtggccg tggacgagca
      721 cgacgcgcgt cgaggcgacg ttcaatgcga gacggaacaa ggtcgcgagc agcagcacgg
      781 tcgggaaggc cgagaaatcg agcggtttcg acgcctgcag cgcgaccatc aggatcagga
      841 ggctgatcat gatgttggcg acgaagctga tgtcgaggat cagcggcgaa accgggatca
      901 ccatcagccc gacgacaatc agcatccccg ccggcagcgc cgaaacgctg gcgaaacgcg
      961 agaaattgcg aaggttgaag ccggcggtca tgccgcgcct cccgaaagcg acgcgagccg
     1021 ccgataggca tcggcggcaa agcccatcga gagcttcttt ggcataggct ggatatccat
     1081 catctggagc ggcgggcgac ctccaccggc ggggctcgcg aggccgctcg agctgctcgc
     1141 ctgcgcgtgc cagctcgagg gagcgaaggg cttcatattt tcggtcttgc cgccctcttc
     1201 gagcttcacg cgaaaacgtt cgcggatctc ggcgaggctg cgcatgctgc cgtcggcggc
     1261 atagaatacc gagcggttgg cggccgccgc ctcgggcatc atcgacgcgc cgggctggtc
     1321 gggattggcc gcgagcgcag tcaggaattt ggcggcgccg ccgcttccga ggaaatgcgc
     1381 gagatagagg tcgaccggct cggcaccgcg gccgatccgg ctttccagat aggcgcgatt
     1441 gtcgccggtc aacgcggcgg ccatgttcga ggaggcggcg ggatcgtcgc gcaggtcgaa
     1501 gatctgctgg cgaagcgcgg gatcggcgac gctcagccgg cccg
//
LOCUS       DATMSU010000166         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_135546,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000166 DATMSU010000000
VERSION     DATMSU010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135546"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN034_04305"
     CDS             <1..223
                     /locus_tag="VN034_04305"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541692.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWV59850.1"
                     /translation="AARALDLDADIPVVTLATAHPAKFREAVERATGVRPPLPARLGN
                     LFEREERYTKLPGDYDAVKAFILAEAARG"
     gene            228..1106
                     /locus_tag="VN034_04310"
     CDS             228..1106
                     /locus_tag="VN034_04310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV59851.1"
                     /translation="MPLVTLVGEAWDDYGLVDSGNGRKLERYGRYRFIRPEPQAMWAP
                     ALPASEWEAADGEFIPASDDDGGGRWYYNKPVPAEGWPLGWRETRFTAQCTPFRHLGF
                     FPDMAPVWDWLRERVADKPDPAFLNLFGYTGVGSQALAASGASVTHVDASKKSVGQAR
                     ENAALAGMADKPVRWIVDDAGKFTAREVRRERRYDAILLDPPKFGRGPGGERWQIEEG
                     LGPLLADCRRLLDADSRALFLTVYAVRMSALAIGELLAQLFADLPGQVECGELAVREE
                     ARGLLLPTAIFARWSR"
     gene            1280..4321
                     /locus_tag="VN034_04315"
     CDS             1280..4321
                     /locus_tag="VN034_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59852.1"
                     /translation="MRASQKQWLAKFLAGTSALSMMGVAQVAAAQDAPADSGVNGDEI
                     VVTGIRASLAASADIKREAQGVVDAISAEDIGKFPDTNLAESLQRITGVSIDRQNGEG
                     SLVTVRGFGPEFNLVTVNGRQVPTAVIGDGGSAPSSRSFDFANLASEGIAAVEVYKSG
                     RATIESGGIGSTINIRTPRPLDNPGMRGSLSVKGVYDTSRNKGDTVTPEVSGIFSTTF
                     ADDTIGIMVAGSYQKRKSSTNTANVGWRDGYLGSENNWGSLAFPTRIVNGVEEPDPRY
                     PNITNRPEGDDVYQVPQNASYDLNDINRERINGQAVLQFRPTDGLTATVDYIYSRNTV
                     ETRNSNVGVWFNHDNTSSAWTDGPVAGPIFYSEIFGPPTGTPGPNALYGGKDLSYSGA
                     LTENRAENNSLGFNLEWNGTDGVSFELDAHHSTAEVNPVNRFGSSMSLGNAVFGVRSQ
                     RIDFDNDLPVISYDMYPGIDPLDASLITPTGNAFRNAFFRNRINQVQLRGQYDHDGGF
                     LDSIDFGVSYVDSKVRSAFGTIQNDDTWGGAGPASSIPDDIFSLVTLPDKFPGLAQDG
                     MIQSFYSFDFERMADLVEQNFQTCSNPATGSAQPGTCLANFNTDRRITEKTLAPYLQV
                     ATVFDLFQNPAHFTAGVRYETTDIASSALVPVPVTTTWVGDNEFNVVFSGESDFTRFK
                     GSYDNWLPAVDFDISPMQDVKLRASYSHTITRPDYASMQGGRTIDTLFRVGGGTGAQG
                     NPGLIPYKSKNIDLSAEWYYARDSYLSVGYFHKDVKNFISSTRIDSNAFGLTTPVGGP
                     RWNAAIAAIGSSATASQIRQYIFENFPETTTIDGGGGGQPFTGIINAAPGDPLVNFEI
                     NTPVNSDQTASLNGWEFALQHSFWDTGFGVILNYTIVNGDATYDNTQPSSVPQFALTG
                     LSDSANAVAFYDKNGLQARVAWNWRDEFLAGTGPNPFYTEEYWQIDASASYEFIPGLT
                     GFVEAINLTGEGRRGHLRHKNNVTFVQPGFARYAAGVRFSF"
     gene            4426..>4757
                     /locus_tag="VN034_04320"
     CDS             4426..>4757
                     /locus_tag="VN034_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HWV59853.1"
                     /translation="MSSAIERVVIVGGGTAGWLTASRLASSPRATAGRLSVTLIEAPD
                     IPTIGVGEGTWPTMRATLAAIGIGEGEFLTACDASFKQGSRFDGWVTGAADDRYLHPF
                     TPPPAAPTA"
BASE COUNT          864 a         1538 c         1529 g          826 t
ORIGIN      
        1 cgccgcgcgc gcgctcgacc tcgatgccga tattccggtc gtcacgctcg cgacggcgca
       61 tccggcgaaa ttccgcgaag cggttgagcg cgcgaccggc gtgcgcccgc cgctgcccgc
      121 gcggctcggc aatctgttcg agcgcgagga gcgctatacg aagctgccgg gcgactatga
      181 cgcggtgaag gcgttcatcc tggcggaagc cgcgcgtggc tgatcttctg cccctcgtca
      241 cgctcgtcgg cgaggcgtgg gacgattacg ggctggtcga cagcggaaac gggcgcaagc
      301 tcgaacgcta tgggcgctat cgcttcatcc gccccgagcc gcaggcgatg tgggcgcccg
      361 cgctgcccgc cagcgaatgg gaagccgccg acggtgaatt catcccggcg tcggacgacg
      421 atggcggcgg gcgttggtac tacaacaaac ccgtgcctgc cgaaggctgg ccgctaggct
      481 ggcgcgaaac gcgcttcacc gcgcaatgca cgccgttccg ccatttgggt ttctttcccg
      541 acatggcgcc ggtgtgggat tggttgcgcg aacgcgttgc cgacaagccc gacccggcct
      601 tcctcaacct gttcggctat accggcgtcg gcagccaggc gctcgccgcc tcgggcgcgt
      661 ccgtcaccca tgtcgatgcg tcgaagaagt cggtggggca ggcgcgcgaa aatgccgcgc
      721 ttgccggcat ggccgacaag ccggtgcgct ggatcgtcga cgatgcgggc aaattcaccg
      781 cgcgtgaggt gcggcgcgag cggcgctacg acgcgatcct gctcgacccg ccgaagttcg
      841 ggcgcggccc gggaggtgag cggtggcaga tcgaggaggg gcttgggccg ctgctcgccg
      901 actgccgccg cttgctcgat gccgacagcc gggcgctgtt cctgaccgtc tatgccgtgc
      961 gcatgtcggc gctagcgatc ggcgaattgc tcgcgcagct tttcgccgac ctgccgggcc
     1021 aggtcgaatg cggagaactc gcggtgcgcg aggaagcgcg ggggttgctg ctgccgaccg
     1081 cgattttcgc gcgctggagc cgctgaaacc gggatcgacg cgcctgttat tctgcatacg
     1141 tttgcggcga atattctgta acattgtgac cgaaatgcca cgctcgacgc gttgacaatg
     1201 agaacgttcc catttagata acgttcacat aacgggaatg ccgccaccaa aagagagcgg
     1261 ctgagtggga ggaatgggga tgcgggcgtc tcaaaaacag tggcttgcca aatttctggc
     1321 cgggacttcg gcgctgtcga tgatgggcgt ggcgcaggtc gcggccgcgc aggacgcgcc
     1381 ggccgacagc ggagtgaacg gcgacgagat cgtcgtcacc ggcatccgtg cctcgctcgc
     1441 cgcatcggcc gacatcaagc gcgaggcgca gggcgtcgtc gatgcgattt ccgccgaaga
     1501 tatcggcaaa ttccccgaca ccaacctcgc cgaatcgctc cagcgcatca ccggcgtgtc
     1561 gatcgatcgc cagaatggcg agggttcgct ggtgaccgtt cgcggcttcg gacccgaatt
     1621 caacctcgtg accgtcaacg gccgccaggt gccgaccgcg gtgatcgggg acggcggcag
     1681 cgcgccgtcg tcgcgctcat tcgatttcgc caacctcgcg tccgaaggca tcgcggcggt
     1741 cgaagtctac aagagcgggc gcgcgacgat cgagtcgggc ggtatcgggt cgacgatcaa
     1801 catccgcacc ccgcgcccgc tcgacaatcc gggcatgcgc ggcagcctgt cggtcaaggg
     1861 tgtctatgac acgtcgcgta ataagggcga tacggtaacg cccgaagtgt cgggcatttt
     1921 cagcaccacc ttcgccgacg acacgatcgg catcatggtc gcggggtcgt atcagaagcg
     1981 caagtcgagc acgaacaccg ctaacgtcgg ctggcgcgat ggctatctcg gcagcgagaa
     2041 caactggggt tcgctggcgt ttccgacccg gatcgtcaac ggcgtcgaag agcccgaccc
     2101 gcgctatccc aatatcacca accgccccga gggtgacgac gtctatcagg tgccgcagaa
     2161 cgcatcctat gacctcaacg acatcaatcg cgagcgcatc aacgggcagg cggtgctgca
     2221 attccggccg accgacgggc tgacagcgac cgtcgactac atctattcgc gcaacaccgt
     2281 cgaaacgcgg aacagcaatg tcggcgtgtg gttcaaccac gacaatacgt cgagcgcctg
     2341 gaccgatggt ccggtcgccg gcccgatctt ttacagcgag attttcgggc ccccgacagg
     2401 aacgccggga ccgaatgcgc tctatggcgg caaggatctg tcgtacagcg gtgcgctgac
     2461 cgaaaaccgg gcggaaaata attcgctcgg cttcaatctc gaatggaatg gcacggacgg
     2521 cgtgtcgttc gaactcgacg cgcatcattc gaccgccgag gtcaatccgg tcaaccgctt
     2581 cgggtcgagc atgtcgctgg gtaacgccgt gttcggcgtg cgcagccagc ggatcgattt
     2641 cgacaacgac ctgcccgtga tttcctatga catgtatccg ggcatcgacc cgctcgacgc
     2701 atcgctgatc acgccgaccg gcaacgcctt ccgcaacgcc tttttccgca accggatcaa
     2761 ccaggtgcag ctgcgcggcc aatatgacca tgacggcggt ttcctcgaca gcatcgattt
     2821 cggcgtctcc tatgtcgaca gcaaggtgcg ctcggcgttc ggcacgatcc agaacgacga
     2881 tacctggggc ggcgcggggc cggcttcatc cattcccgac gacatcttct cgctcgtcac
     2941 gcttcccgac aaattcccgg ggctcgcgca ggatgggatg atccaaagct tctacagctt
     3001 cgatttcgag cgcatggcgg atctggtcga gcagaatttc cagacgtgca gcaaccccgc
     3061 gacgggctcg gcccagccgg gaacctgcct tgcgaacttc aacaccgacc ggcgcatcac
     3121 cgaaaagacg ctggcgccct atctgcaggt cgcgaccgtt ttcgacctgt tccagaaccc
     3181 cgcgcatttc acggcgggtg tccggtacga gacgaccgac atcgcctcgt ccgcgctggt
     3241 accggtgccg gtcacgacca cctgggtcgg cgacaatgaa ttcaacgtcg tcttttcggg
     3301 cgagagcgac ttcacccggt tcaagggctc ctacgacaat tggctgcccg cggtcgattt
     3361 cgacatctcg ccgatgcagg acgtcaagct gcgcgcatcc tacagccaca cgatcacgcg
     3421 ccccgactat gccagcatgc agggcggacg gacgatcgat acgctgttcc gcgttggcgg
     3481 cggcacgggc gcgcagggca acccggggct gatcccctat aagtcgaaga atatcgacct
     3541 gtccgccgag tggtattacg cccgcgattc ctatctgtcg gtcggctatt tccacaagga
     3601 tgtgaaaaac ttcatctcct cgacgcggat cgactcgaac gcgttcgggc tgacgacgcc
     3661 ggtcggcggg ccgcgctgga atgccgccat cgccgccatc ggatcgagcg cgaccgcgtc
     3721 gcaaatccgg cagtatatct tcgagaattt ccccgaaacg acgacgatcg acgggggcgg
     3781 gggcggtcag cccttcaccg gcatcatcaa cgccgcgccg ggcgatccgc tggtcaattt
     3841 cgaaatcaac acgccggtga acagcgatca gaccgcatcg ctcaacggct gggaattcgc
     3901 gctccagcac agcttctggg ataccggttt cggtgtgatc ctgaactata cgatcgtcaa
     3961 cggcgacgcg acatacgaca atacgcagcc gtcgtcggtg ccgcagttcg cgctgaccgg
     4021 actcagcgac agcgccaatg ccgtggcctt ctatgacaag aacgggcttc aggcgcgtgt
     4081 tgcatggaac tggcgcgacg aattcctcgc agggacgggg cctaacccct tctataccga
     4141 ggaatattgg cagatcgacg ccagtgcgag ctatgagttc atccccgggc tcacgggctt
     4201 cgtcgaggcg atcaacctga cgggtgaggg acgccgcggg catcttcgcc acaagaacaa
     4261 cgtcaccttc gtccagccgg gcttcgcccg ctatgcggca ggggtcaggt tcagcttcta
     4321 atgtggcggg ctgtttccgc cgggtcgcag gcaagggggt cgcgcccttc gaggcgcggc
     4381 tcttttgcgt gacggctgtg gacgcttggc ctaggatcga cggcaatgag tagcgcgatc
     4441 gagagggttg tcatcgtggg cggcgggacc gccggctggc tgacggcatc gcgcctcgcc
     4501 agttcgccgc gcgcgacggc gggccggctc tcggtcacgc tgatcgaagc acccgacatt
     4561 ccgacgatcg gcgtcggcga gggcacttgg ccgacgatgc gtgcgaccct cgcggcgatc
     4621 ggcatcggcg agggcgaatt cctcaccgcg tgcgacgctt cgttcaagca gggatcgcgg
     4681 ttcgacggct gggtcaccgg cgccgccgac gatcgctatt tgcacccctt cacgccgccg
     4741 cctgcggcgc caacggc
//
LOCUS       DATMSU010000167         5009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_107512,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000167 DATMSU010000000
VERSION     DATMSU010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107512"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /gene="glpD"
                     /locus_tag="VN034_04325"
     CDS             complement(<1..1362)
                     /gene="glpD"
                     /locus_tag="VN034_04325"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HWV59854.1"
                     /translation="MPDSAPPYDVIVVGGGVNGAGVARDAAGRGARVLLLEAGDLAQG
                     TSSKSTKLIHGGLRYLEHYEFGLVREALKERERLWGIAPHIIHPMRFVLPYRDGLRPR
                     WLLRLGLFLYDHIGGREKLPATRSVDLRRHAAGAPLQPQYIRGFEYSDGWVDDARLVA
                     LNARDAADRGARVRTRTRAEMLRCEDGLWIVDAVGDQGQHYRFTGRSVVNAAGPAVLD
                     LLRRADAEPDHRMRLVRGSHIVVRKLFDHDYAYFFQLPDGRIFFAIPYERDFTLIGTT
                     DQDHDGPASEAQASDEEIAYLCEGASLYFRERVTPADVVWTYSGVRPLIEDGSGRPEA
                     ATRGYRIDLDLDEGAPLLTIYGGKITSYRHVAEDAVDELADHVPALSGKKWTAKAPLP
                     GGNFPVDGAGALRAKYKLAHPFLPAATVDRIVRAYGTDARRWLADAGDWDALGGEIAH
                     GLSV"
     gene            1603..3066
                     /gene="zwf"
                     /locus_tag="VN034_04330"
     CDS             1603..3066
                     /gene="zwf"
                     /locus_tag="VN034_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWV59855.1"
                     /translation="MSVKVETLVLFGATGDLAQRMLFPSLYNLHLDGLLADALTIIGS
                     GRSKMDAAAFREQVQGALAEHLPADRIDDAGVASFLGRIDYCAIDAGAGTGYDELAAL
                     LGDRMGRPIGVYLSTPPSMFGPIAQGLKAANIACAECRIAMEKPIGHDLASSREVNAE
                     VGDAFAEDRVFRIDHYLGKETVQNLLALRFANMLFEPLWNAQAIDHVQITVAETVGLE
                     GRVSYYDGVGALKDMVQNHMLQLLAIIAMEPPSSVSSTAVRDEKVKLLRSLRKMNAED
                     VKAHSVKGQYTSGAVNGGAVAGYADELGDPSNTETFVALKAYIDNWRWKGVPFYLRTG
                     KRMPQRKSEVLIQFKPVPHNIFARVGAGKLNANSMIINLQPEENIRVKVMAKQPGLDR
                     DGVKLKEVTMDVSLSHSFAGERRRIAYERLLLDFIEGDQTLFVRRDEVEAQWQWIDSI
                     RDAWAAVDMAPQNYTAGSWGPSSAIALIERDGASWHD"
     gene            3262..>5009
                     /gene="edd"
                     /locus_tag="VN034_04335"
     CDS             3262..>5009
                     /gene="edd"
                     /locus_tag="VN034_04335"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HWV59856.1"
                     /translation="MTTLHPTITAVTDRIVARSAQRRAAYLDLMDRQRDAGTNRGNLS
                     CGNLAHGFAASGEDKPVIRTGGAMNIGIVTAYNDMLSAHQPYGRYPEQIKLFAREVGV
                     TAQVAGGVPAMCDGVTQGQAGMDLSLFSRDNIAQGTAIALSHAMFEGALLLGICDKIV
                     PGLLIGALRFGHLPQILVPAGPMPSGLANKEKQRVRQLYAEGKASRDELLEAEAASYH
                     GAGTCTFYGTANSNQMMMELMGLHVPGSAFTNPGTKLRQELTRAAAHRIAAIGWDGDD
                     YRPLGRCIDEKAIVNAAIGLLATGGSTNHAIHLPAIARAAGIIIDWQDFDELSHAVPL
                     LARVYPNGAGDVNNFHAAGGIGYVVRELLDAGLLHGDVLTIGGTMADYAAEPVLVNDE
                     LHWQAAPAESRDDTMLRPVAAPFSPDGGMRLLAGNLGRAIIKTSAVAEDRWTIEAPCR
                     IFDDQNQVLTAFKAGELERDVVVVVRFQGPRANGMPELHKLTPALGVLQDKGFRVALL
                     TDGRMSGASGKVPAVIHLSPEALPGPDGVSGPLAYLQDGDIVRVCAVNGEAVALVDAA
                     EWASRTPAAAPPPAVGV"
BASE COUNT          861 a         1671 c         1642 g          835 t
ORIGIN      
        1 gacgctgagg ccatgcgcga tctcgccgcc gagcgcgtcc cagtcgcccg cgtccgccag
       61 ccagcggcgc gcatcggtgc cataggccct gacgatgcga tcgaccgtcg cggcgggcag
      121 gaaggggtgg gcgagcttat atttggccct cagagccccg gcgccgtcga ccggaaaatt
      181 gccgcccggc agcggcgcct tcgccgtcca ctttttgccc gaaagcgcgg gaacatggtc
      241 cgcgagttcg tcgaccgcat cttcggcgac atggcgatag ctggtgatct tgccgccata
      301 gatggtgagc agtggcgccc cctcgtccaa atcgaggtcg atccggtagc cgcgcgtcgc
      361 ggcttcgggg cgccccgatc cgtcctcgat cagcggccgc acgcccgaat aggtccagac
      421 cacgtcggcg ggcgtcactc gctcgcggaa atagaggctc gcgccttcgc agagataggc
      481 gatctcttcg tcgctcgcct gggcctcgct cgcggggccg tcatggtcct ggtcggtcgt
      541 tccgatcagc gtgaaatcgc gctcataggg aatcgcgaag aaaatgcgcc cgtcgggcag
      601 ctggaagaaa taggcgtaat catggtcgaa tagcttgcgc acgacgatgt gcgagccgcg
      661 gacgagccgc atccggtgat cgggttcggc atcggcgcgc cgcagcagat cgagcaccgc
      721 gggccccgcg gcgttgacga cgctgcgccc cgtaaagcga taatgctgcc cctgatcgcc
      781 caccgcatca acgatccaca gcccatcctc gcagcgcagc atttcggcac gggtgcgcgt
      841 tcgcacccgt gcgccgcggt cggcggcatc gcgcgcgttg agcgcgacga gccgggcatc
      901 gtcgacccag ccgtccgaat attcgaaccc gcgaatatat tgcggctgga gcggcgcgcc
      961 cgcggcatgg cggcggagat cgaccgagcg cgtcgcgggc agcttctcgc ggccgccgat
     1021 atggtcatag agaaacagcc cgagcctgag cagccagcgc ggccgcaggc cgtcgcgata
     1081 gggcaggaca aagcgcatcg ggtggatgat atgcggcgcg atgccccaca atcgctcgcg
     1141 ctccttcagc gcttcgcgca ccaggccgaa ttcataatgt tcgagatagc gcagcccgcc
     1201 gtggatgagc ttggtcgatt tcgacgacgt gccttgggcg aggtcgccgg cttctagcag
     1261 caggacccgc gccccgcgcc cggcggcatc gcgcgcgacg cccgcgccat tgacgccgcc
     1321 gccgacgacg atgacatcat agggaggggc gctgtcgggc atcgggtggc gtcttagaat
     1381 ccttcctatt ccatttccat atgtcactgt gcccctgcga aggcaggggc ctatctccga
     1441 tgccatcgct atcgaatacc ggttgcttgt tgtcgaagcg ccggggagat gggcccctgc
     1501 ctccgcaggg gcacggcaac aatggacgac cggcttacgt atgcgcgttg cattcgcttc
     1561 gtgtatcccc caattccctg cctgttatcc aaggggacga atgtgagcgt gaaagtcgaa
     1621 acattggtgc tgttcggcgc gacgggcgac cttgcgcagc gcatgctctt tccctcgctc
     1681 tacaatctac acctcgacgg gctgctcgcc gacgcgctga cgatcatcgg atcgggacgt
     1741 tcgaagatgg acgccgcggc gtttcgcgaa caggtgcagg gcgcgctcgc cgagcatctg
     1801 cccgccgacc ggatcgacga tgcgggcgtc gcgagctttc tgggccggat cgactattgc
     1861 gcgatcgatg ccggcgcggg caccggatat gacgaattgg cggcgctgct cggcgaccgt
     1921 atgggccgcc cgatcggcgt ctatctttcg accccgccgt cgatgttcgg tccgatcgcg
     1981 cagggtctga aggccgcgaa catcgcctgc gccgaatgcc gcatcgcgat ggaaaagccg
     2041 atcggccacg atctcgcctc gtcgcgcgag gtcaatgccg aggtcggcga cgcctttgcc
     2101 gaggaccgcg tctttcgcat cgaccattat ctcggcaagg aaaccgtcca gaatctcctc
     2161 gcgctgcgtt tcgccaacat gctcttcgag cctttgtgga acgcgcaggc gatcgatcat
     2221 gtccagatca ccgtcgccga aaccgtcggg ctcgaaggcc gcgtttccta ttatgacggc
     2281 gtcggcgcgc tcaaggacat ggtgcagaac catatgctcc agctgctcgc gatcatcgcg
     2341 atggagccgc cgtcgagcgt ctcttcgacc gcggtgcgcg acgaaaaggt caagctgctg
     2401 cgtagcctgc gcaagatgaa cgccgaggat gtgaaggcgc acagcgtcaa ggggcaatat
     2461 accagcgggg cggtgaacgg cggcgcggtc gcgggctatg ccgacgagct cggcgatccg
     2521 tcgaacaccg aaaccttcgt cgcgctgaag gcctatatcg acaattggcg ctggaagggc
     2581 gtgccctttt acctgcgcac cggcaagcgg atgccgcagc gcaagtccga agtgctgatc
     2641 cagttcaagc ccgtcccgca caatatcttc gcgcgcgtcg gcgcgggcaa gctcaacgcg
     2701 aacagcatga tcatcaacct ccagcccgag gaaaatatcc gggtcaaggt gatggcgaag
     2761 cagccgggcc tcgaccgcga cggggtgaag ctgaaagaag tgacgatgga cgtctcgctc
     2821 tcgcacagct tcgccggcga gcgccgccgc atcgcctacg aacgcctgct gctcgatttt
     2881 atcgagggcg accagaccct gttcgtgcgc cgcgacgagg tcgaggcgca gtggcagtgg
     2941 atcgattcga tccgcgatgc ctgggcggcg gtcgatatgg cgccgcagaa ctacaccgcg
     3001 ggaagctggg gaccgtcgag tgcgatcgcg ctgatcgagc gcgacggagc gagctggcat
     3061 gattgagacc gccccaaacc ttctcccctt gggggagaag gatacgaagc cttgtcgcgc
     3121 agcgactagg cgcagttgga tgagggtctc cgcctttgct ttcgccgctg ccgttcacac
     3181 cctcacccag cttcgactag cgagcaagct cgcaagtctg cgcatccctc tcccccaagg
     3241 ggagagggtt ttggaattgt catgaccact cttcacccca ccatcaccgc ggtcaccgac
     3301 cgcatcgtcg cccgcagcgc ccaacgccgc gccgcctatc tcgacctcat ggaccgccag
     3361 cgcgacgcgg ggaccaaccg cggcaacctg tcgtgcggca acctcgcgca cggctttgcc
     3421 gcctcgggcg aggacaagcc cgtgatccgc accggcggcg cgatgaacat cggcattgtc
     3481 accgcgtaca acgacatgct ttcggcgcat cagccctatg gccgctaccc cgagcagatc
     3541 aagctgttcg cgcgcgaggt cggcgtcacc gcgcaggtcg cgggcggcgt gcccgcgatg
     3601 tgcgacgggg tgacgcaggg gcaggcgggc atggacctgt cgctgttcag ccgcgacaat
     3661 atcgcgcagg gcaccgcgat cgcattgagt catgcgatgt tcgagggcgc actgctgctc
     3721 ggcatctgcg acaagatcgt ccccggcctg ctgatcggcg cgctgcgctt cggtcatctg
     3781 ccgcagatcc tcgtccccgc cgggccgatg ccctcggggc tcgcgaacaa ggaaaagcag
     3841 cgcgtccgcc agctctatgc cgagggcaag gcgagccgcg acgaactgct cgaagccgag
     3901 gcggcgagct atcatggcgc gggcacctgc accttctatg gcactgcgaa ctcgaaccag
     3961 atgatgatgg agctgatggg gctgcacgtc cccggcagcg ctttcactaa tccgggtacg
     4021 aaactccgtc aggaactgac gcgcgccgcg gcgcaccgta tcgccgcgat cggctgggat
     4081 ggcgacgatt atcgcccgct tggccgctgc atcgacgaaa aggcgatcgt caacgccgcg
     4141 atcgggctgc tcgcgaccgg cggctctacc aaccatgcga tccaccttcc cgcgatcgcg
     4201 cgcgcggcgg ggattatcat cgactggcag gatttcgacg agctcagcca cgcggtgccg
     4261 ctgctcgcgc gcgtctatcc gaacggcgcg ggcgacgtga acaatttcca cgccgcgggc
     4321 ggcatcggtt acgtcgtgag ggaattgctc gacgccgggc tgctccacgg cgatgtgctg
     4381 accatcggcg gcacgatggc cgattatgcc gccgagcccg tactcgtgaa cgacgaactc
     4441 cactggcaag ccgcgcctgc cgagagccgc gacgatacga tgctccgccc ggtcgcggcg
     4501 cctttctcgc ccgacggcgg gatgcgtctg ctcgcgggca atctcggccg tgcgatcatc
     4561 aagacgagcg cggtcgccga ggaccgctgg acgatcgagg ccccgtgccg gatcttcgat
     4621 gatcagaacc aggtgctcac cgcgttcaag gcgggcgagc tcgaacgcga tgtcgtcgtc
     4681 gtcgtgcgct ttcaggggcc gcgcgccaac ggcatgcccg aactgcacaa gctgactccg
     4741 gccttgggcg tgttgcagga caagggcttt cgcgtcgcct tgctcaccga cggccgcatg
     4801 tcgggcgcga gcggcaaggt gcccgcggtg atccacctct cgcccgaagc cttgcccggc
     4861 cccgacgggg tgagcggccc gctcgcctat ctgcaggacg gcgacatcgt tcgcgtctgc
     4921 gcggtgaacg gcgaagcggt tgcgctcgtc gatgcggccg aatgggcatc gcgcacgccg
     4981 gctgccgcgc cgccgccagc ggtcggcgt
//
LOCUS       DATMSU010000168         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_78598, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000168 DATMSU010000000
VERSION     DATMSU010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78598"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..624
                     /locus_tag="VN034_04340"
     CDS             94..624
                     /locus_tag="VN034_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWV59857.1"
                     /translation="MIKRLISLLFLAWVLGFAWFALLLPLPADAQKTDAIVVLTGGPG
                     RIDRALERLEAGDAKRLLISGVAREVKPRELAAEYKRPVSLFDCCIALGFEAEDTRSN
                     ATEVATWAQRRKYKSIRLVTTDWHMRRAEYEIGRAVGDTVTILPDAVRSQPSFATLFR
                     EYHKYLAGLAGGLLGL"
     gene            636..1373
                     /locus_tag="VN034_04345"
     CDS             636..1373
                     /locus_tag="VN034_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWV59858.1"
                     /translation="MRYTIALIRSILFWILFFVMSSISSIGAVISLPISHHGTIFFVR
                     IWAQFHRLICRFVLGQKIVIDGEMPDIPVLYVFKHEGAFETVEQPMLFKHPAVFAKEQ
                     LFSIPVWGQAARFYGLVPVDRDGGGKAMRAMLNAAKAALAGGRPLVLFAEGTRIPHGL
                     APPLRSGFAGIYRLLGVPVIPVAVNSGLVYPPRRWVKWSGTITYRVGETIPAGLPREE
                     AEERVWRAINALNPPEALLEGYKNPSP"
     gene            complement(1545..>2268)
                     /locus_tag="VN034_04350"
     CDS             complement(1545..>2268)
                     /locus_tag="VN034_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HWV59859.1"
                     /translation="RSDADLVILAVPVGRMADAAAAIAPGLKAEAIISDVGSSKAGVA
                     DALAKALPDHIVIPAHPVAGTENSGPAAGFATLFDGRWCIVTPGAGTPDEAVASVTGF
                     WQALGAKVDVMDAAHHDMVLAVTSHLPHLIAYTIVGTASELEEVTESEVIKYSAGGFR
                     DFTRIAASDPVMWRDVFLANKDAVLATLQRFNEDLTALQQAIRRGDGAKLEDWFTRTR
                     AIRRSIIEQGQDDAAPDFGRKH"
BASE COUNT          372 a          763 c          721 g          412 t
ORIGIN      
        1 ctgggcgctc ttgctggcgc tgccgctatt ggctatcgcg ctcgctgccc tcaccgcgcg
       61 gcagaccctg ctcgcggcgc tcaagaagat tctatgatca agcgcctgat ttccctgttg
      121 tttctggcct gggtgcttgg cttcgcatgg tttgcgctgc tgctgccgct gcccgccgac
      181 gcgcaaaaga ccgatgcgat cgtcgtgctc accggcggcc ccggccgcat cgaccgcgcg
      241 cttgaacggc tcgaggcggg cgacgcaaag cgcctgctca tcagcggtgt cgcgcgcgag
      301 gtgaagccgc gcgagctggc cgccgaatat aagcgcccgg tttcgctgtt cgactgctgc
      361 atcgcattgg ggttcgaggc cgaggatacg cgctcgaacg cgaccgaggt cgcgacctgg
      421 gcgcagcggc gcaagtacaa gagcatccgg ctggtcacca ccgactggca tatgcgccgc
      481 gccgaatatg aaatcggccg cgcggtcggc gacacggtga cgatcctgcc cgacgcggtg
      541 cgcagccagc ccagtttcgc cactctgttt cgcgaatatc ataaatatct cgcgggactg
      601 gcgggcgggc tgctgggtct ctaagaccag cgccgatgcg ctatacgatc gcccttatcc
      661 gctcgatcct gttctggatc ctgttcttcg tcatgagcag catctcgtcg atcggcgcgg
      721 tgatctcgct tccgatctcg caccacggga cgattttctt cgtccgcatc tgggcccagt
      781 tccaccggct gatctgccgc ttcgtcctcg ggcaaaagat cgtcatcgac ggcgagatgc
      841 ccgatattcc ggtgctttat gttttcaagc acgaaggcgc gttcgagacg gtcgaacagc
      901 cgatgctgtt caaacatccc gcggtgttcg ccaaggagca gcttttctcg attcccgtct
      961 ggggtcaggc cgcgcgtttt tacgggctcg tgcccgtcga ccgcgacggt ggcggaaagg
     1021 cgatgcgcgc gatgctgaac gccgcgaagg ccgcactcgc gggcggccgg ccgctcgtgc
     1081 tgttcgcgga ggggacgcgc attccacatg ggctcgcacc gccgctgcgc tcgggtttcg
     1141 caggcattta tcgcctgctc ggcgtgccgg tgatcccggt cgcggtaaac agcggcctcg
     1201 tctatccacc gcgccgctgg gtcaaatggt cgggcacgat cacctatagg gtcggcgaaa
     1261 cgatccccgc cggcctgccg cgcgaagaag ccgaagagcg cgtgtggcgc gcgatcaatg
     1321 ccctgaaccc gcctgaggcg ttgctggaag gctacaaaaa cccttcccca tagatggggg
     1381 aaggatacga agccttatcg cgaagcgatt aggcgaagtt ggatgggggt gatggcgcgt
     1441 ccatacaccg ccgagccagg ccgagaccgc acccccaccg ctgcgactag ccagcaagct
     1501 gccaagtctc gctgcctccc ccatcaaggg ggaggatgac agtatcaatg tttccgcccg
     1561 aaatcgggcg ccgcatcatc ctgcccctgc tcgatgatcg accgccggat cgcgcgcgtg
     1621 cgcgtgaacc agtcctcgag cttcgcgccg tcaccgcgcc ggatcgcctg ctggagcgcg
     1681 gtcagatcct cgttgaagcg ctgcaaggtc gcgagcaccg catccttgtt ggcgaggaac
     1741 acgtcgcgcc acatcaccgg atcgctcgcc gcgatgcgcg tgaagtcgcg gaaaccaccc
     1801 gccgaatatt tgatcacctc gctctcggtg acctcttcga gttcgctcgc ggtgccgacg
     1861 atcgtatagg cgatgaggtg cggcaaatgg ctcgtcactg cgagcaccat gtcgtggtgc
     1921 gcggcgtcca tgacgtcgac cttcgcgccg agcgcctgcc agaagcccgt caccgacgcc
     1981 accgcctcgt cgggcgtgcc ggcgcccggg gtaacgatgc accagcggcc atcgaacagc
     2041 gtcgcgaaac cggcggcggg tccgctgttc tcggttcctg cgaccggatg cgccggaatg
     2101 acgatatgat cgggcagcgc cttcgccagc gcgtcggcga cccccgcctt cgacgatccg
     2161 acgtccgaaa tgatcgcctc cgccttcagc cccggcgcga tcgccgccgc tgcgtcagcc
     2221 atgcgcccga ccggaaccgc gaggatgacg aggtcggcgt cagatcgg
//
LOCUS       DATMSU010000169         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_88497, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000169 DATMSU010000000
VERSION     DATMSU010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88497"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VN034_04355"
     CDS             <1..859
                     /locus_tag="VN034_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59860.1"
                     /translation="ARPANTPGNARPEASDLQFASEKVDAYELGVKWDGPGIDVNLAA
                     FWQEYSNYQLNTFNGVNFEVTNIQACKDDLGTGPIDNSAATGGCASDRLKPGVVAKGF
                     EIETFLRPARYLSVNMGLTYVDTLYRRNLVGTGGRPLSPVLFQLPGRGVSNAAKYVAT
                     AGISWTPPIGDSGLSALVYFDTRLQSDTNTGSDLDVEKIQDGFAVFNGRIGLYGRDRR
                     WGIELWGQNLFNKQYYQIGADMPLQGSGSFRSVAAPAALGFPATANQLFVGFPGEPRT
                     YGVTLRGQF"
     gene            complement(1032..2687)
                     /locus_tag="VN034_04360"
     CDS             complement(1032..2687)
                     /locus_tag="VN034_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWV59861.1"
                     /translation="MKTHLAAALLLSTAFAAPTWAQTGIDAANLTGTVRTLASDLFQG
                     RAPGTVGEERTIGYLIGRLEAMGLEPAGVDGGWTQPVPLLHTRLGSPETLAFDRKGAA
                     TPLSFGTDIYVSTLQPKDKAVVANAPMVFVGYGVSAPERGWDDFKGQDLKGKVAVFLI
                     NDPDFVAAKGEDAFGKFGGRTMTYYGRWTYKFEEAARRGAIAALIVHDAEGVGYGWNV
                     VKSGGGENYGLVVPPEKVTSLALQGWISGEMATKLFAGAGQDLEKLRVAARRKDFKPI
                     DLGTSFDAAVPVTQEVVQSQNVLAKIPGAKRPDEVVIYGAHWDAYGEGPPDEQGRIYR
                     AGANDDALGVAGLFEIARLFKAAPPPDRTIAFAFWTAEERGLLGSEAYAQAPLFPLEK
                     TVANLGLDILQTAGRAKDVVLVGKGQGTLEDDLARFAVAQGRSVTPESLPERGLFYRA
                     DHFSLAKRGVPVLLMMGIAGASDLVEGGKEAGQKWVDAYTGKCYHQACDAWGPDWNLD
                     GAVQDIDIFYRIGDELARSARWPGWKAGSEFKAIRDESAAARK"
     gene            complement(2806..>3006)
                     /locus_tag="VN034_04365"
     CDS             complement(2806..>3006)
                     /locus_tag="VN034_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV59862.1"
                     /translation="GRTAIVIAHRLSTVRTLDRILVFDQGRVIEDGDHETLLANADGQ
                     YRRLFDRQSGNIVDEAPALVGS"
BASE COUNT          482 a         1008 c         1003 g          513 t
ORIGIN      
        1 cgcgcggccg gcgaacacgc ccggcaacgc gcggcccgaa gcgagcgacc tgcaattcgc
       61 gagcgagaag gtcgacgcct atgagctcgg cgtcaaatgg gacggtcccg ggatcgacgt
      121 caatctggcg gccttctggc aggagtatag caattaccag ctcaacacct tcaacggcgt
      181 caatttcgag gttaccaata tccaggcgtg caaggacgat ctggggaccg ggccgatcga
      241 caacagcgcc gcgaccggcg gctgcgcgtc cgaccggttg aagcccgggg tcgtcgccaa
      301 ggggttcgag atcgagacct tcctgcgccc cgcacgctat ctctcggtca atatggggct
      361 gacctatgtc gacaccctct atcgccgcaa cctcgtcggc accggcggcc ggccgctgtc
      421 gccggtgctg ttccagctgc ccgggcgcgg cgtgtcgaac gccgccaaat atgtcgcgac
      481 tgcgggaatc agctggacgc cgccgatcgg cgacagcggg ctgagcgcgc tcgtctattt
      541 cgatacccgg ctccagagcg acaccaacac cgggtcggat ctcgatgtcg aaaagatcca
      601 ggatggcttc gcggtcttca acggccgcat cggcctttat gggcgcgatc gccgctgggg
      661 gatcgagctg tgggggcaga atctattcaa caagcaatat taccagatcg gcgccgacat
      721 gccgctgcag ggatcgggat cgttccgttc ggtcgcagcg cccgcggcgc ttggcttccc
      781 ggcgaccgca aaccagctgt tcgtcggctt ccccggcgag ccgcgcacct atggcgtgac
      841 cctgcgcggc cagttctgac cgtctctccc cctccccaaa gtcggggagg gcgtagggag
      901 ggcctgccgc ggccgggcac tgtcgcgaca ggccctttcc ctttttggga gaggcggttt
      961 ttgggcgggg agcggatggc ttcttttgag aaggagcaac cggccgccag ccgccctcaa
     1021 cgcatggcgg cctatttgcg cgccgcggca ctctcgtcgc ggatcgcctt gaattcgctg
     1081 cccgccttcc agccgggcca gcgcgccgag cgtgcgagtt cgtcaccgat ccggtagaaa
     1141 atatcgatgt cctgaaccgc gccgtcgagg ttccagtcgg ggccccaggc gtcgcacgcc
     1201 tgatgatagc atttgcccgt ataggcatcg acccacttct ggccggcttc cttgccgccc
     1261 tcgaccagat cggacgcgcc cgcgatgccc atcatcaaca ggacgggaac gccgcgcttg
     1321 gcaaggctga aatggtcggc gcgatagaag aggccgcgtt cgggaaggct ctcgggcgtc
     1381 acgctgcgcc cctgcgccac cgcgaagcgc gcaagatcgt cctcgagcgt gccctgtccc
     1441 ttgccgacga ggacgacgtc cttggcacgg ccggcggtct ggaggatgtc gaggccgaga
     1501 ttggcgacgg tcttttcgag cgggaagaga ggcgcctgcg cataagcctc ggatccgagc
     1561 aggccgcgct cctcggcggt ccagaaagcg aaggcgattg tgcggtcggg cggcggcgcg
     1621 gccttgaaca gccttgcgat ctcgaacagc cccgcgacgc cgagcgcatc gtcgttggca
     1681 cccgcgcgat agatgcggcc ctgctcgtcg ggcggacctt cgccataggc gtcccagtgc
     1741 gcgccataga tcaccacctc gtcgggccgt ttcgcgccgg ggattttggc gaggacattc
     1801 tggctctgca cgacttcctg cgtcaccggg accgcggcgt cgaagctcgt gccgaggtcg
     1861 atcggcttga aatccttgcg ccgcgccgcg acccgcagtt tctccaaatc ctgcccggcg
     1921 ccggcgaaga gtttcgtcgc catctcgccc gagatccagc cctgcagcgc gaggctcgtc
     1981 accttttcgg gcggaacgac gaggccgtaa ttttcgccgc cgccgctttt gacgacgttc
     2041 cagccatagc cgacgccctc ggcgtcgtgg acgatcagcg ccgcgatcgc gccgcggcgc
     2101 gcggcctcct cgaacttgta ggtccagcgg ccgtaatagg tcatcgtccg gccgccgaat
     2161 ttgccgaacg cgtcctcgcc cttcgccgcg acgaaatcgg ggtcgttgat caggaagacc
     2221 gcgaccttgc ccttcaaatc ctgccccttg aaatcgtccc agccgcgttc gggggcgctg
     2281 acgccgtaac cgacgaagac catcggcgcg ttcgcgacca ccgccttgtc cttgggctgg
     2341 agcgtcgaga catagatgtc ggtgccgaag ctcagcggcg tcgcggcgcc cttgcggtcg
     2401 aaggcgagcg tttcgggact accgaggcgc gtgtggagca agggcacggg ctgggtccag
     2461 ccgccgtcga cccccgcggg ttcgagcccc atcgcctcca gccgcccgat gagatagccg
     2521 atcgtgcgct cctcgcccac cgtgccgggg gcgcggccct gaaacaggtc ggacgcgagc
     2581 gtgcgcacgg ttccggtgag gttggcggcg tcgattcccg tttgcgccca ggtcggcgcg
     2641 gcgaaggcgg tcgagagaag gagggcggcg gcaaggtgcg ttttcatgag gctttccggt
     2701 ttgtcagcgg tcgaggcagc gtttgagata ggcgaatgcg ccgtcgaagt cgcgcatttc
     2761 catccggaaa atcggggcgc cggtcaagcg gcgccccgga tcgccctagc tcccgacgag
     2821 cgcgggcgcc tcgtcgacga tattgcccga ctggcggtcg aacagccggc gatactggcc
     2881 atcggcgttc gcgagcagcg tttcatggtc gccatcctcg atcacgcggc cctggtcgaa
     2941 aaccagaatc cggtcgagcg tgcgcaccgt cgacagccgg tgcgcgatga cgatcgcggt
     3001 gcggcc
//
LOCUS       DATMSU010000170         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_88502, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000170 DATMSU010000000
VERSION     DATMSU010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88502"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VN034_04370"
     CDS             complement(<1..677)
                     /locus_tag="VN034_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59863.1"
                     /translation="MSRGFTADDVSVQTGRTFLVTGANAGIGFEASRILAARGAHVIL
                     ACRDENKAEVAMNRIWTDVPKAELSFQPLDLADLDQVRDAAKAVLAGPRIDVLVNNAG
                     VMIPPRTLTKQGYELQFGVNHLGTFALTGLLHPHVDDRIVITGSIAHRNGTMDYSDLS
                     ASRSYHNWPRYQMSKLANLLFMFELDRRLSAAGRATQAIGCHPGVALTELTRHLPLPL
                     RSMTPLAS"
     gene            complement(702..1256)
                     /locus_tag="VN034_04375"
     CDS             complement(702..1256)
                     /locus_tag="VN034_04375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWV59864.1"
                     /translation="MIVGVDEAGRGPLAGPVVAAAVLLCEGGIVGLDDSKKLTAKRRG
                     ELEIEIKARCRWGVGEASVAEIDRINILQATFLAMTRAVEALGFEPAEILVDGNRLPK
                     WRYTATAIIGGDALHPCISAASILAKEHRDRFMVGVARDFPGFGWETNMGYGTAYHLA
                     ALRKHGPTPHHRTSFAPVAQMQLV"
     gene            complement(1372..>1768)
                     /locus_tag="VN034_04380"
     CDS             complement(1372..>1768)
                     /locus_tag="VN034_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59865.1"
                     /translation="VRNDGLFTGPALNQITLPAKDYAVSVAFYKQLGLTQIVDSPDNG
                     YARFEAANGVTLSIHVGDGAAGGAAAYLESGALDAWVAYLARRGVRFDQMPKDEEWGW
                     REARLSDPAGNRLCLYQAAEYRRYPPWRV"
BASE COUNT          285 a          610 c          567 g          306 t
ORIGIN      
        1 gacgcgaggg gggtcatcga ccgtagcggc agcggcaggt gccgcgtcag ttcggtgagc
       61 gcgacgccgg gatggcaacc gatcgcctgc gtcgcccggc ccgcggcgct cagccggcgg
      121 tcgagctcga acatgaagag caggttcgcg agcttgctca tctggtagcg cggccagttg
      181 tgatagctgc gcgacgccga caggtcgctg taatccatcg tcccgttccg gtgcgcgatg
      241 ctgcccgtga tgacgatgcg gtcgtcgaca tgcggatgga gcagcccggt caaggcgaag
      301 gtgccgaggt gattgacccc gaattgcagc tcatacccct gtttggtcag cgtccggggc
      361 gggatcatca cccccgcatt gttcaccagc acgtcgatcc gcggccccgc gagcaccgcc
      421 ttcgccgcat cgcgaacctg gtcgaggtcg gcgaggtcga gcggctggaa cgacagttcg
      481 gctttcggca cgtcggtcca gatgcggttc atcgccacct cggccttgtt ttcgtcgcgg
      541 cacgcgagaa tcacatgcgc gccgcgcgcg gcgagaatcc tggaggcttc gaatccgatc
      601 cccgcattgg caccggtgac aaggaaggtg cggccggtct ggaccgacac atcgtcagcc
      661 gtaaatcccc tgctcatcgg cttcccctcc ggtttcgggt ttcagaccag ctgcatctgt
      721 gcgaccgggg caaaactggt ccgatggtgc ggcgtgggcc catgcttgcg cagcgccgcg
      781 agatgatatg cggtgccata gcccatattc gtttcccagc caaaacccgg aaagtcgcgc
      841 gccaccccga ccatgaagcg gtcgcgatgt tccttcgcga ggatgctcgc cgccgaaatg
      901 cacggatgca acgcatcgcc gccgatgatc gcggtcgcgg tatatcgcca cttgggcagc
      961 cggttgccgt cgacgagtat ctcggcgggc tcgaacccca gcgcctcgac cgcgcgcgtc
     1021 atcgcaagga aggtcgcctg caatatgttg atgcggtcga tctcggcgac gctcgcctcg
     1081 ccaacgcccc agcggcagcg cgccttgatc tcgatctcga gctcgccgcg gcgcttggca
     1141 gtgagtttct tcgaatcgtc gagcccgacg attccgccct cgcacagcag caccgccgcc
     1201 gcgaccaccg gcccggcgag cggcccacgc ccggcttcat cgacgccgac gatcactgat
     1261 tcccctccct ttttagggag gggttagggg tgggtagccg caggcattct tccttggacg
     1321 ccacacccac ccccagcccc tccctgggag ggaggggagt ttcgggttgg cttagacgcg
     1381 ccacggcgga tacctccgat attcggccgc ctgatagagg cagagccggt tgcccgcggg
     1441 atcgctcagc cgcgcctcgc gccagcccca ttcctcgtcc ttcggcatct ggtcgaagcg
     1501 caccccgcgc cgcgccagat aagcgaccca cgcgtcgagc gcgccgctct ccagataggc
     1561 ggccgccccg cccgccgcgc cgtcgccgac atggatcgac aaggtgacgc cgttcgccgc
     1621 ctcgaaccgc gcatagccat tgtcggggct gtcgacgatc tgggtcagtc cgagttgctt
     1681 gtagaaagcg accgacaccg cataatcctt cgccggcagc gtgatctggt tgagcgccgg
     1741 gccggtgaac agcccgtcgt tgcgcaca
//
LOCUS       DATMSU010000171         7436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_12555, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000171 DATMSU010000000
VERSION     DATMSU010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12555"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VN034_04385"
     CDS             complement(<1..157)
                     /locus_tag="VN034_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV59866.1"
                     /translation="MPLFDLTGKVALVIGANTGIGQGIALALAEAGADIAAAGRSPAH
                     ETVGKVRG"
     gene            complement(299..1141)
                     /gene="kduI"
                     /locus_tag="VN034_04390"
     CDS             complement(299..1141)
                     /gene="kduI"
                     /locus_tag="VN034_04390"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HWV59867.1"
                     /translation="MFSKTYYATHPVMMEEVSNAALRDRYLIQNLFRTGECVLNYTHA
                     DRFVIGGVAVGSAPVKLPAQSEPPSAAGHPFLERRELAIVNVSSVEGTVTVDGESFTL
                     GNKDCLYVTMGAKDVAFAGEGARYYLASCPAHKAFTTRKLGIADANALDRGSLAESNE
                     RTIFQLVIPGVCDSAQLVMGLTVLKEGSVWNTMPPHIHERRSEIYFYFELDNVDTDRV
                     MHFMGEGEATRHIVMQNEEAVISPPWSIHMGAGTKSYAFIWAMAGENQDYTDMNVLDI
                     CQLS"
     gene            1313..2368
                     /locus_tag="VN034_04395"
     CDS             1313..2368
                     /locus_tag="VN034_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWV59868.1"
                     /translation="MAAQNKGPGGKQPTINDVAALAGVSKKTVSRVINRSEFLTDKTR
                     AAVEKAIEQLGFVPNPQARALAFRRNFLIALLHDNPNAQTVLNFQRGVLDAIKDSDLA
                     LLVRPVDRGSDKMLDDVRTFLEKQRPIGAMLLPPISENDDLAALCEDLGVRYVRIGSA
                     RIDDAKRCISSNDREVVAEAVRGLVALGHRRIGFVRGPVGFRSAAEREEGFREALAEA
                     GLDLPAELYAPGNYRYTAGIEAGEALLSLAAPPTAVFCSNDEMAAGLMSVAHGKGIKV
                     PGELSIIGFDDSPTATHIWPALSTVRWPIREMGIRAAQTLVPDFLGPGAKAAGDESNV
                     LASTFVERQSVAPPPGR"
     gene            complement(2372..4051)
                     /locus_tag="VN034_04400"
     CDS             complement(2372..4051)
                     /locus_tag="VN034_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59869.1"
                     /translation="MTSVDRRARLVSVLALGAALIFPVQAQQAKLPKILSDPAIMAAN
                     PLPDFSYAGYGFGLALIPDDAGTVIEVTDHGAIPDDGLDDSKAVLRALAAANAVKGKV
                     TLRFPKGRTQITEILRITRSDIILDGAGDGPGGSELWFPRPLKLIDKSHDYDELRAYL
                     VREKKQQVEPEHNIDYLFTEYTWSGGMIYVAPAGSRPVSYDGTKDVRDPVLATGVSGQ
                     QFARTLTVDDPSKLKVGDVVQLQWFSVDGPESKILKSLYGDTDLPIGSHHWTFPNRPV
                     VAQATRVAAIRGKTVTLGDPLLHDIRADQPAVVAGWKHLTNVGIQNLRLQFPEAPAFG
                     HHLEQGYNGIYMTGVFDGWIRGLTIDRADSGILTDNAASLTISDIITTGDRRAHYSVH
                     VGAVHNVLVKDLKVENPAVHPLSINTRSTRSVFLRAVVDRDAIIDQHSGSNHQNLFDQ
                     VEMHIDPKRGRDGWQYQLWVGGGAPYWKPGHGLYNTLWNAKLVMPESVPADATVTITS
                     GLEGPGERIVGLHANRKIAVRYNPAPYIEGTGQAVGAVPSLYDYQLARRRR"
     gene            complement(4137..6752)
                     /locus_tag="VN034_04405"
     CDS             complement(4137..6752)
                     /locus_tag="VN034_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV59870.1"
                     /translation="MAIQAGRGIGMMAKLAGGASLAALAMAPAHAQDAVAAPAEEDAI
                     VVTGIRASLEQAADIKREADQVLDVITAEDVGKLPDANVAEALQRVTGVQITRVFGEG
                     QSVSVRGLQQVRVEVDGRTLLGWSARLSPPENDQLGRSSGLDSVPSSLFGRLEVRKSP
                     LASQAEGGLGGTVNLVTPKPLSFKEPTVSLRAQGVYSENSDKFEPAITGFATTKFSDG
                     RIGVMLAGEYQKRTSTTQAFERNNFLDRNYTAGGTTTVQQTPVLLQYEQFTVDRSRLG
                     LNGAIQFAVTPEFTVTADALYSKLKTGRRQDFFAFRLPTGTNPVTNAVVEDGMVVAGN
                     ANGTVTTAGQIRNEPTESFLYGLNGKYESDGLTIEADGYYSKGTIDQTIQIITLQAAA
                     PVPGAFDFRDQTIPSLTLGGTFDPADYASYNPANNGVRSNRLLGLLEEWTGKLDVAYE
                     TAGGVTIAAGVRYTDLHARSNAFRSQVTPTREEIEPYLKRIDAGDFLADIPGTFPNSF
                     LSTAPTYDYVFNRAQAAEPNPDPDGRSALLPNLQRDYDFSEKTLAGYLMVSGEGEIAG
                     IPYRANAGVRIASTRLSVDSYVNVGAVSTLRNDRNRYTNVLPSANIAFNVTDDFLVRV
                     SGSQTMQRASIADLAPSTFFNATNLSVTGGNVNLEPPIATQADVSFEYYTGKSSLISG
                     AVFYKDVKNFIASFVTTGVDLDLDDQGRELTFSRPENLASAKIKGFEIGIQQFMDFLP
                     APLDGFGVIANYTYSDAEDNAGFPLVAVSKNSYNLVGLYEKGPFSARIAYNYRDEAVF
                     EFSQGRPSFIGARSQLDAQLGVDITKNIALSLQAQNLLPKKSATSEYSNFNTTALNSY
                     ALSERRFSVGVRAKF"
     gene            7107..>7436
                     /locus_tag="VN034_04410"
     CDS             7107..>7436
                     /locus_tag="VN034_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWV59871.1"
                     /translation="MLDKRPLLRAGALCAIALSLAACSAADEKGGRSAPEVGYVVAAI
                     QPVPVTTSLGGRTVAYETSEVRPQVNGLIRRRLFTEGSLVRAGAPLYQIDARLYQAGV
                     DQAAANLA"
BASE COUNT         1265 a         2351 c         2454 g         1366 t
ORIGIN      
        1 gtccgcggac cttgccgacg gtctcgtggg cgggcgagcg gcccgcggcg gcaatgtcgg
       61 caccggcttc ggcgagcgca agcgcgatcc cctgcccgat gccggtgttg gcgccgatga
      121 cgagcgcgac ttttcctgtg aggtcgaata atggcatcgt tggtctcaat cctctcgtca
      181 tgctgaactt gtttcagcat ccatggcctg tgtcctcgtc cggcgcggcg ctttttggga
      241 aagaccggcc atggaccctg aaacaagttc agggtgacga agaaaactgg cgtgttcctt
      301 acgacagctg acagatgtcg aggacgttca tgtcggtata atcctgattc tcgcccgcca
      361 tcgcccagat gaaggcatag gatttcgtgc ccgcgcccat atggatcgac cagggcggcg
      421 agatcaccgc ttcctcattc tgcatcacga tgtggcgcgt cgcctcgccc tcgcccatga
      481 agtgcatgac gcggtcggtg tcgacattgt cgagttcgaa atagaaataa atctcgctgc
      541 ggcgctcgtg gatgtgcggc ggcatcgtgt tccagacgct gccttccttg agcacggtga
      601 ggcccatgac gagctgcgcg ctgtcgcaga cgccggggat gacaagctgg aagatcgtgc
      661 gctcgttcga ttcggcgagg ctgccgcgat cgagcgcatt ggcgtcggcg atgccgagct
      721 tgcgcgtggt gaacgccttg tgcgccggac acgacgccag atagtagcgc gcgccctcgc
      781 ccgcgaaggc gacgtccttc gcgcccatcg tcacatagag gcaatccttg ttgccgagcg
      841 tgaagctctc gccgtcgacg gtcaccgtgc cctcgaccga cgagacgttg acgatcgcga
      901 gctcgcggcg ttcgaggaag ggatggcccg cggccgacgg cggctcgctc tgcgcgggga
      961 gtttgaccgg cgccgagccc acggcgaccc cgccgatcac gaagcggtcg gcgtgggtgt
     1021 agttgaggac gcattcgccg gtacggaaca gattctggat caggtagcga tcgcgcagcg
     1081 cggcattcga cacctcctcc atcatcacgg gatgggtggc gtaataggtc ttggaaaaca
     1141 tgggatcgca tcctcgctcg atatttctgg gcttgccgcc ttgctagttt tgatggtaac
     1201 cggtgtcaag aggtgttgac acgttcgcgg cgcggttgat tttacgcgtt cgagcgccca
     1261 tcagcataga aagcgcgagc ctggccagag gtttgcaggg gaaagagcaa atatggcggc
     1321 acaaaataag gggccggggg gcaagcagcc gacgatcaac gatgtcgcgg cgctggcggg
     1381 agtgtcgaag aagacggtga gccgggtgat caaccggtcc gaattcctga ccgacaagac
     1441 gcgcgcggcg gtcgaaaagg cgatcgagca gctcggcttc gtccccaatc cgcaggcgcg
     1501 cgcgctcgcc tttcgccgca acttcctgat cgcgctgctc cacgacaatc cgaacgcgca
     1561 gaccgtgctc aatttccagc gcggggtgct cgacgcgatc aaggacagcg acctcgcgct
     1621 gctcgtccgc cccgtcgacc gcgggtcgga caagatgctc gacgacgtcc gcaccttcct
     1681 tgaaaagcag cgcccgatcg gcgcgatgct gctgccgccg atttcggaga atgacgatct
     1741 cgccgcgctc tgcgaggacc ttggcgtgcg ttatgtccgc atcggttcgg cgcggatcga
     1801 cgatgcgaag cgctgcatct cgtcgaacga ccgcgaggtg gtcgccgagg cggtgcgcgg
     1861 gctcgtcgcg ctcggccacc gccggatcgg cttcgtgcgc ggccccgtcg gcttccgctc
     1921 ggccgccgag cgcgaagagg gtttccgcga ggcgctcgcc gaggccgggc tcgatcttcc
     1981 cgccgaactt tatgcgcccg gcaactaccg ttacactgcg gggatcgagg ccggcgaagc
     2041 cctgctctcg ctggccgcgc cgccgaccgc ggtcttctgt tcgaacgacg agatggcggc
     2101 gggcctgatg agcgtcgcgc atggcaaggg catcaaggtg ccgggcgagc tgtcgatcat
     2161 cgggttcgac gacagcccga ccgcgacgca tatctggccc gcgctcagca ccgtgcgctg
     2221 gccgatccgc gaaatgggca tccgcgccgc gcagacgctc gttcccgact tcctcggccc
     2281 cggcgccaag gcggcgggcg acgaaagcaa tgtgctcgcc tcgacctttg tcgagcggca
     2341 atcggtcgcg ccgccgccgg ggcgctgacc ctcagcgccg ccggcgcgcg agctgatagt
     2401 cgtagagcga cgggaccgcg ccgaccgcct gccccgtgcc ttcgatatag ggcgcgggat
     2461 tgtagcgaac cgcaatcttg cggttggcgt ggaggccgac gatccgctcg cccggccctt
     2521 cgagcccgct ggtgatcgtc accgtcgcgt cggcgggaac gctctcgggc atcaccagct
     2581 tcgcgttcca gagcgtgttg tagaggccgt gacccggctt ccagtaaggc gcgccgccgc
     2641 cgacccagag ctgatactgc cagccgtccc tgccccgttt cggatcgatg tgcatttcga
     2701 cttggtcgaa cagattctga tggttcgacc cgctgtgctg gtcgatgatc gcatcgcggt
     2761 cgacgaccgc gcgcaggaac accgagcgcg tcgagcgcgt gttgatgctg agcgggtgga
     2821 cggcggggtt ttcgactttc agatccttga ccagcacatt gtgcaccgcg ccgacatgca
     2881 ccgaataatg cgcgcggcgg tcgcccgtcg ttatgatgtc ggaaatcgtc aggctcgcgg
     2941 cattgtcggt caggatgccg ctgtccgccc ggtcgatcgt cagtccgcgg atccagccgt
     3001 cgaacacgcc ggtcatatag atgccgttat agccctgctc cagatgatgg ccgaaggcgg
     3061 gcgcttcggg aaattgcagg cgaaggttct ggatgccgac attggtcaga tgcttccagc
     3121 cggcgaccac cgcgggctgg tcggcgcgga tgtcgtggag cagcggatcg ccgagcgtca
     3181 ccgtcttgcc gcggatcgcc gcgacgcgcg tcgcctgcgc gaccaccggc cggttcggga
     3241 aggtccagtg gtgcgagccg atcggcaggt cggtgtcgcc gtaaagcgat ttcaggatct
     3301 tgctctccgg tccgtcgacc gaaaaccatt gcagctggac gacgtcgccg accttgagct
     3361 ttgacggatc gtcgaccgtc agcgtgcggg cgaactgctg gcccgatacc ccggtcgcga
     3421 gcaccgggtc gcgcacatcc ttcgtcccgt cgtacgagac cgggcggctg cccgcgggcg
     3481 cgacatagat catcccgccc gaccaggtat attcggtgaa gagatagtcg atattatgct
     3541 caggctcgac ctgctgcttc ttctcgcgca ccagatatgc gcgcagctcg tcataatcgt
     3601 ggcttttgtc gatcagcttg agcgggcgcg gaaaccagag ctcgctgccg cccgggccat
     3661 cgcccgcgcc gtcgaggatg atgtcgctgc gcgtgatgcg caatatttcg gtgatctgcg
     3721 tgcgcccttt cgggaaacgc agcgtcacct tgcccttcac cgcattggcc gcggcgagcg
     3781 cgcggagcac cgccttgcta tcgtcgagcc cgtcgtcggg aatcgcgcca tggtcggtca
     3841 cctcgatgac cgtcccggca tcatcaggga tgagggcgag gccgaaacca tagcccgcat
     3901 agctgaaatc gggcagcgga tttgcggcca tgatggcggg gtccgagagg atcttaggca
     3961 atttcgcttg ctgcgcctga accggaaaaa tcagcgccgc gcccagcgcc agaaccgaaa
     4021 cgaggcgcgc acgccgatca accgaagtca ttactcattc actccctgcg gcccctttgg
     4081 catggcggcg cgcgacccgc aaaaggtcgg ggacgccgcc atgccgggga cctccattaa
     4141 aacttcgcgc gcacgccgac cgagaaacgc cgctcggaaa gggcgtagct gttgagcgcg
     4201 gtggtattga aattgctata ttcgctcgtc gccgattttt tcggcagcag attctgcgcc
     4261 tgcaaggaca gcgcgatatt cttcgtgatg tcgaccccga gttgcgcgtc gagctgcgag
     4321 cgcgcgccga tgaagctcgg gcgtccttgc gaaaattcga acaccgcctc gtcgcgataa
     4381 ttatatgcga tgcgcgccga gaagggcccc ttctcataga ggccgacgag attgtagctg
     4441 ttcttcgaca ccgcgacgag cgggaagccg gcattatcct cggcatccga gtaagtgtag
     4501 ttggcgatca cgccgaagcc gtcgagcggc gcgggcagaa agtccatgaa ctgctggatg
     4561 ccgatctcga accccttgat cttcgcgctc gccagatttt cgggacgcga gaaggtgagc
     4621 tcgcggccct ggtcgtcgag atcgagatcg accccggtgg tcacgaagct cgcgatgaaa
     4681 ttcttgacgt ccttgtagaa gaccgcgccc gagatcagcg agcttttccc cgtataatat
     4741 tcgaagctga catcggcctg cgtcgcgatc ggcggctcga gattcacatt gccgccggtg
     4801 accgacaggt tggtcgcgtt gaagaaggtc gacggcgcga ggtcggcgat cgaggcgcgc
     4861 tgcatcgtct gcgaccccga cacgcggacg aggaaatcgt cggtgacgtt gaacgcgata
     4921 ttcgcgctcg gcaggacgtt ggtatagcga ttccggtcgt tgcgcagcgt actgaccgcc
     4981 ccgacattga cataggagtc gaccgagagg cgggtcgacg cgatacgcac gcccgcattg
     5041 gcccggtacg gaatgccggc gatctcgccc tcgcccgaca ccatcagata gcccgcgagc
     5101 gtcttttcgc tgaagtcata gtcgcgctgg agattgggca gcagcgccga gcggccgtcg
     5161 ggatcgggat ttggctcggc cgcctgcgca cggttgaaga catagtcgta ggtcggcgcg
     5221 gtgctcagga agctgttcgg gaaagtgccc ggaatgtccg cgaggaaatc gcccgcgtcg
     5281 atgcgcttca gatagggttc gatctcctcg cgggttggtg tgacctggct gcggaaggcg
     5341 ttcgagcgcg cgtgaaggtc ggtatagcgc acccccgccg cgatcgtgac gccgccggcg
     5401 gtttcgtagg cgacgtcgag cttgccggtc cattcctcta gcagcccgag caggcggttg
     5461 ctgcgcacgc cgttgttcgc agggttgtag gaggcgtaat cggccgggtc gaaggtcccg
     5521 ccgagcgtca gcgaggggat cgtctgatcg cggaaatcga aggcgcccgg aacaggcgcg
     5581 gccgcttgca gcgtaatgat ctggatcgtc tggtcgatcg tgcccttgct gtaatagccg
     5641 tcggcctcga tcgtcagccc gtcgctctca tatttgccgt tgagtccgta gagaaagctc
     5701 tcggtcggct cgttgcggat ctgccccgcg gtggtcacgg tgccgttcgc attgcccgcg
     5761 acgaccatgc catcctcgac gaccgcgttc gtcaccggat tggtgccggt gggcaggcgg
     5821 aaggcgaaga aatcctggcg gcgcccggtc ttgagctttg aatagagcgc gtcggccgtc
     5881 acggtgaatt cgggcgtcac cgcaaactgg atggcgccgt tgagcccgag ccgcgagcgg
     5941 tcgacggtga attgttcata ttggagcagc accggcgtct gctggacggt cgtcgtcccg
     6001 ccggcggtat aattgcggtc gaggaaattg ttgcgctcga acgcctgcgt cgtcgaggtg
     6061 cgcttctgat attcgcccgc gagcatgacg ccgatgcggc cgtcagagaa tttggtcgtc
     6121 gcgaaaccgg tgatcgcggg ttcgaacttg tcgctgttct ccgaatagac gccctgcgcg
     6181 cgcagcgaga ccgtgggctc cttgaacgac agcggcttcg gcgtgacgag gttgaccgtg
     6241 ccgccgagcc cgccctcggc ctggctcgcg agcggcgact tgcgcacttc cagacgcccg
     6301 aacaggctcg acggcaccga gtcgaggccc gacgagcggc cgagctggtc attctcgggc
     6361 ggcgacagcc gcgccgacca gccgagcaaa gtgcgaccgt cgacttcgac gcggacctgc
     6421 tggagcccgc gcaccgacac cgactggcct tcgccgaaga cgcgggtgat ctggacgccg
     6481 gtgacgcgct ggagcgcctc ggcgacgttg gcgtcgggaa gcttgcccac gtcttcggcg
     6541 gtgatcacgt cgagcacctg atcggcttcg cgcttgatgt cggccgcctg ttcgagcgac
     6601 gcgcgaatac cggtgacgac gatcgcatcc tcttcggccg gcgcggcgac cgcgtcctgc
     6661 gcatgcgccg gcgccatcgc cagcgcggcg agcgacgcgc cgccggcgag ctttgccatc
     6721 atcccgatgc cccgtcctgc ctgaatagcc atatccatcc tcccacccgg gcgcgggact
     6781 tgccgcccga gcgcccgttt tccacggatt gcgaccaagg ccgcctttcg gagccgacca
     6841 gtcgcccatc tatttctatt tcgttgtccg gctttggtaa ccggtgtcac gatgatgcgg
     6901 gcaagagcga gttactgtca agcaaaaaat tgtatgacaa ctatcccatt agtatgacaa
     6961 ctttgcggat ccggaacctc gtcacaggcg cggcggaaaa attgtcgcaa attgtcgcat
     7021 cgcgcccaga gggcttgcca gcgcgggggc aatgggctga ataggcgcaa aactcccccg
     7081 ttccgatttg aaacgagatt aaccccatgc tggacaagag gccgctgctt cgtgccggag
     7141 ccctgtgtgc catcgcgctg tcgcttgccg cctgctccgc cgcagacgag aagggcgggc
     7201 gcagcgcgcc cgaggtcggc tatgtcgtcg ccgcgatcca gccggtgccc gtcaccacct
     7261 cgctcggcgg acgcaccgtc gcttatgaaa cgagcgaagt gcgcccgcag gtgaacggcc
     7321 tgatccgccg ccgcctgttc accgagggaa gcctcgtgcg cgccggcgcg cccctctatc
     7381 agatcgacgc gcggctttat caggcggggg tcgaccaagc cgccgcgaac ctcgcg
//
LOCUS       DATMSU010000172         7151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_78659, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000172 DATMSU010000000
VERSION     DATMSU010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78659"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1294)
                     /locus_tag="VN034_04415"
     CDS             complement(50..1294)
                     /locus_tag="VN034_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWV59872.1"
                     /translation="MHRRQFLGTAALTGLAATVPAPVFAADAAGLPNLAAKAIPIGKA
                     ERVARIAKAKELMRANDIGALLIEPGSSLIYFTGVRWSRSERLTAAVLTREGEVAVVT
                     PFFEEPSVRESLGIDAEVLTWNEDDNPLAAVAAWLGKRGLAKGRIGVEETVRYFAVDG
                     LEKAMPQATVVNGAPVVRGCRMHKSPAEIALMQIAADITLAAYRHTAPRIEAGMTPAD
                     IGAVMKAATIALGGKSEFELILLGEASAYPHGSGKPQAVKSGEVVLMDCGATVHGYQS
                     DISRSFVYGKASARQRQVWDQMRKGQDVAFAAAKLGTPAGKVDDAVRAYYESLGWGPG
                     YKLPGTSHRTGHGIGLDGHEPVNLVRGETTKLAPGMCFSNEPGIYIPGEFGIRLEDCF
                     YMTDGGPKYFSEPPPSIDQPFA"
     gene            complement(1311..2297)
                     /locus_tag="VN034_04420"
     CDS             complement(1311..2297)
                     /locus_tag="VN034_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59873.1"
                     /translation="MAAAISIALAFPTASCSAEPSATPAVEREIITLPFPGGTDIERL
                     KQSHRDVISLLRKRAAEEARDPEYQPQVGVYSIFPIDSSRPRIIIGAVTPRKGVFYAM
                     PLGHYASLDDYKNGRIERKDKIDLCRMEMQWDRLPGGPIAPGLNWAGMYMSDLDCKPP
                     PADQLAMLDEWKSRKAKPDYVVEDKVSAGEVSVTLIREVRPKASAYRPFDPVAASAEV
                     MEAGDGVTWAIPLIDENWAASVVAMRSGRLFAYFSDRIYAGDSQDLMCVADGLAWADI
                     MAEPALLATQVTRQFCQDLMKQYSDRRRDEMRRADEKLPPPTIQSIPMTPRN"
     gene            complement(2333..3538)
                     /locus_tag="VN034_04425"
     CDS             complement(2333..3538)
                     /locus_tag="VN034_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HWV59874.1"
                     /translation="MTTLFGIDRLLADPALRKPLEGKRVALLAHPASVTADLTHSLDA
                     LVAAGVNVTAVFGPQHGVRGDLQDNMMESPDFTDPTYGMPVFSLYGEVRRPSGQSMQT
                     FDVILIDLQDLGCRIYTYATTLLYILEAAAAHRKAVWVLDRPNPAGRPVEGTRLLPGW
                     ESFVGAGPMVMRHGLTMGEMGHWFIRHFGLDVDYRVIEMAGWEPEGPGFGWPTTRVWI
                     NPSPNAANLNMARAYAGTVMVEGATLSEGRGTTRPLELFGAPDIDAKAVIAEMQRIAP
                     TWLAGCKLRDIWFEPTFHKHMKQLNSGVHIHAEGGWYDHAAFRPWRVQAVGFKAIRSL
                     YPDYPIWRGKDFKYEYTDDVLAIDVINGGPGLREWVDDAGAAPGDLDALALPDEAAWR
                     AEIADLLIY"
     gene            complement(3535..4083)
                     /locus_tag="VN034_04430"
     CDS             complement(3535..4083)
                     /locus_tag="VN034_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HWV59875.1"
                     /translation="MRKELICHPDTPAKAVRAVAVEVSLSFEDGFALRYIVDGAIADL
                     VLPHGEGELVIADSATDGLWESTCFEAFLTEEGQPDYTEFNYSPDGRWACYQFDDYRS
                     LLRSDELAPWEMVAERGEQSYALRIEPGIFPDIGAKLALSAVIEELDGTKSYWALAHP
                     PGKPDFHHPDCFALTLEARGRA"
     gene            4125..5351
                     /gene="tyrS"
                     /locus_tag="VN034_04435"
     CDS             4125..5351
                     /gene="tyrS"
                     /locus_tag="VN034_04435"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWV59876.1"
                     /translation="MTNYKSDLLRLLDERGYIHQTTDAEGLDALAAKQVVPGYIGFDA
                     TAPSLHVGSLVQIMMLRRLQQTGHKPVVLMGGGTTRIGDPTGRDESRKMLSDETIAAN
                     IASIFSIFQQFLSFGDGPTDAVMVDNQDWLGQLGYIELLQEVGTHFTINRMMTFDSVK
                     LRLEREQPMTFLEFNYMILQGYDFRHLSKNRGVRLQMGGSDQWGNIVNGMELGRRMDG
                     ADLFGLTTPLLTTAAGAKMGKTAAGAVWLNPEQLSHFDYWQYWRNCDDRDVGKFLKLF
                     TDLPLEEIARLEALEGAEINEAKKILANEATAMCRSQEAAKAASDTAAQTFEKGQIGG
                     DLPQAVAPAEGMSVVDALRELGFAASNKEARRKLDEGAVKVDGTVVRDPQHLIQPGET
                     PVPLSLGSKKHGLVTR"
     gene            5426..5767
                     /locus_tag="VN034_04440"
     CDS             5426..5767
                     /locus_tag="VN034_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV59877.1"
                     /translation="MRKIDTSKAHYVGLDQRIAPRSDVYCRLPFVMPDGRQEMCTCVN
                     ISADGLLMRFERGLEPGDLVVFRMPIIGRTAAKVVWSLGGKTGVQFDKSISVEDYLPM
                     IRAMGARGDVN"
     gene            complement(5904..>7151)
                     /locus_tag="VN034_04445"
     CDS             complement(5904..>7151)
                     /locus_tag="VN034_04445"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWV59878.1"
                     /translation="MAQDTPMLRMLQGDVGSGKTLVALRAMLSAVEAGTQAALLAPTE
                     ILARQHFATLQRMLGGLPVNLAILTGRDKGKVRESTLMGLTDGSIDILVGTHAIFQQA
                     VTYKDLALVVVDEQHRFGVAQRLMLTQKAARPPHLLVMTATPIPRTLQLANHGEMDVS
                     QIDEMPPGRTPVDTRVISLDRLDDVVGSLDRHLATGAQAYWVCPLVAESEGSELAAAE
                     DRAALLRERLGDARVGLVHGRMKGPEKDEVMARFQAGEIGVLVATTVIEVGVDVPAAS
                     LMIVEHAENFGLAQLHQLRGRVGRGAAKSVCLLLRSQNLSETARERLALMRETNDGFV
                     IAEKDLELRGGGELLGLKQSGDADYRLATPEQLVRLLPVAHDDARLFVERDGGMEGAR
                     GEAVRLCLYLFERDAAVPLLRSG"
BASE COUNT         1285 a         2383 c         2236 g         1247 t
ORIGIN      
        1 gagggtgcgc aaggccgacg gcggcccccg ccttcgcggg ggcgacgggt tatgcgaacg
       61 gctggtcgat cgacggcggc ggttcgctga agtatttcgg cccgccgtcg gtcatgtaga
      121 aacagtcttc cagccttatg ccgaactcgc cggggatata gatgccgggt tcgttcgaga
      181 agcacatgcc gggcgccagc ttcgtcgtct cgccgcgcac caggttgacc ggctcgtgcc
      241 cgtcgagacc gatgccatgc cccgtgcggt gcgaggtgcc gggcagcttg tagccaggcc
      301 cccagccgag gctctcataa taggcgcgta ccgcgtcatc caccttgccc gcgggcgtgc
      361 cgagcttcgc cgcggcgaaa gcgacgtcct gccccttgcg catctggtcc cagacctgcc
      421 gctggcgcgc gctcgccttg ccatagacga agctgcgcga aatgtcggat tgataaccat
      481 gcaccgtcgc gccgcagtcc atcagcacga cttcgccgct cttcaccgct tgcggcttgc
      541 ccgacccatg cggataggcg ctcgcctcgc cgagcaggat cagctcgaat tcgctcttgc
      601 cgccgagcgc gatggtcgcg gccttcatga ccgcgccgat gtcggcgggt gtcatccccg
      661 cctcgatgcg cggtgcagtg tggcgatagg cggcgagcgt gatgtcggcg gcgatttgca
      721 tcagcgcgat ttcggccggc gacttgtgca tccggcaccc gcgcacgaca ggcgcgccgt
      781 tgacgaccgt cgcctgcggc atcgcctttt caagcccgtc gaccgcgaaa tagcgcaccg
      841 tttcctcgac gccgatgcga cccttcgcga gcccgcgctt gcccagccac gctgcgaccg
      901 cggcgagcgg gttatcgtcc tcgttccacg tcagcacctc ggcgtcgatg ccgaggcttt
      961 cgcggaccga cggttcctcg aaaaaaggcg tgacaaccgc gacctcgcct tcgcgggtga
     1021 gcaccgcggc ggtcagccgt tcgctgcgcg accagcgcac cccggtaaaa tagatgaggc
     1081 tcgaccccgg ctcgatcagc agcgcaccga tatcgttcgc gcgcatcaac tccttcgcct
     1141 tggcgattcg cgccacacgc tcggccttgc cgatcgggat ggcctttgcc gcgagattgg
     1201 gaaggcccgc cgcatcggcg gcgaacaccg gggcgggcac ggtcgctgcc agcccggtca
     1261 gcgccgcggt gcccagaaat tgccgccgat gcatcattgt tttctctccg tcagtttcgg
     1321 ggcgtcatgg gtatcgattg gatggtaggg ggcggcaatt tttcatcggc gcgccgcatc
     1381 tcgtccctac ggcgatcgct atattgcttc atcaggtctt ggcagaactg ccgggtgacc
     1441 tgcgtggcga gcagagcggg ctctgccatg atgtcggccc atgccaatcc atcggcaacg
     1501 cacatcaggt cttgggagtc ccccgcgtaa attctgtcgg aaaaataggc aaataatcgg
     1561 ccgctccgca tcgcaacgac cgatgcggcc cagttttcgt cgatcaacgg tatcgcccac
     1621 gtgacaccat ccccggcttc catcacctcc gccgaagcag cgacgggatc gaaagggcga
     1681 tatgccgaag ctttcggccg aacttcccgg atcagcgtaa cgctaacctc acccgccgat
     1741 accttgtctt cgacaacata gtccggcttt gctttgcggc ttttccactc atccaacatg
     1801 gccaattggt cggctggcgg cggcttgcaa tcgaggtccg acatatacat gcccgcccag
     1861 ttaaggccgg gcgcgatcgg cccgcccgga agccggtccc attgcatttc catccggcag
     1921 aggtcgatct tgtccttccg ttcgatccgg ccattcttat aatcgtcgag cgatgcataa
     1981 tggcctaaag gcatggcgta gaacacgccc ttacgtggtg tcaccgcgcc aatgatgatc
     2041 cgcgggcgcg atgagtcgat aggaaaaata gaataaacgc cgacctgagg ctgatattcc
     2101 gggtcgcgcg cttcctctgc tgctcgtttc cgcagcaggc tgataacgtc acgatgcgac
     2161 tgtttcagac gttcgatatc ggtcccgccg gggaacggca gcgtaattat ctcacgctcg
     2221 acagcgggtg tggctgacgg ttcagccgag cagcttgcgg tcgggaatgc aagggcaatg
     2281 ctgatcgcag ccgccaagct tcgctgccaa atcgtccgca ttcgttcgcc cctcaataaa
     2341 tcagcaaatc ggcgatttcc gcgcgccacg ccgcctcgtc gggcagcgcc agcgcatcga
     2401 ggtcacccgg cgccgcgccc gcatcgtcga cccactcgcg caagcccggt ccgccgttga
     2461 tcacgtcgat cgcgagcaca tcgtcggtat attcatattt gaaatccttg ccgcgccaga
     2521 tcggatagtc cggatagagg ctgcggatcg ccttgaagcc caccgcctgc acgcgccacg
     2581 ggcgaaacgc cgcatgatcg taccagccac cctcggcgtg gatatgcacg ccgctattga
     2641 gctgcttcat atgcttgtgg aaggtcggtt cgaaccagat gtcgcgcagc ttgcacccgg
     2701 cgagccatgt gggcgcgatc cgttgcatct ccgcaatcac cgccttggca tcgatatcgg
     2761 gcgcgccgaa cagttcgagc gggcgcgtcg tgcccctgcc ctcgctcagg gtcgccccct
     2821 cgaccatcac ggtgcccgca taggcgcgcg ccatgttgag gttcgcggcg ttggggctcg
     2881 ggttgatcca gacgcgtgtc gtgggccagc cgaagccggg gccttcgggc tcccaccccg
     2941 ccatttcgat cacgcgataa tcgacgtcga gcccgaagtg acggatgaac cagtgcccca
     3001 tctcgcccat cgtgaggcca tggcgcatca ccatcgggcc cgcgccgacg aagctttccc
     3061 agccggggag aagccgcgtg ccctcgacgg gccgcccggc ggggttcggc cgatcgagca
     3121 cccacaccgc cttgcgatgc gccgcggcgg cttcgagaat gtagagcagg gtcgtggcat
     3181 aggtgtagat gcggcagccg aggtcctgca ggtcgatcag gatcacgtcg aacgtctgca
     3241 tcgactgtcc cgacggccgc cgcacctcgc cataaaggct gaacaccggc atgccatagg
     3301 tcgggtcggt gaagtcgggc gactccatca tattgtcctg aaggtcaccg cgcacgccat
     3361 gctgcggccc gaaaaccgcg gtgacattga ccccggccgc gacgagcgcg tcgaggctgt
     3421 gcgtcaggtc ggcggtcacc gaggcgggat gcgcgagcag cgcgacgcgc ttgccttcga
     3481 gcggtttgcg cagcgccggg tcggcgagca ggcggtcgat accgaaaagc gtcgtcatgc
     3541 ccgcccgcgt gcctcaagcg tcagggcaaa gcaatcggga tgatggaagt cgggcttgcc
     3601 gggcgggtgc gcgagcgccc aatagctttt ggtgccatcg agttcctcga tcaccgcgga
     3661 cagcgcgagt ttcgcgccga tgtcgggaaa gatgccgggt tcgatgcgca gcgcatagct
     3721 ttgctcgccg cgctcggcga ccatctccca cggcgcgagt tcgtcggaac gcaagagcga
     3781 gcgataatcg tcgaactgat aacaggccca gcgcccgtcg gggctgtaat tgaattcggt
     3841 atagtcgggc tgcccctcct cggtcaggaa cgcctcgaaa caggtgcttt cccacaggcc
     3901 gtcggtcgcg ctgtcggcga tgaccagctc gccctcgccg tggggcagca cgagatcggc
     3961 gatcgcacca tcgacgatat agcgcagcgc gaaaccatcc tcgaacgaca ggctgacttc
     4021 gaccgcgacc gcgcgcaccg ccttggccgg cgtatcgggg tgacagatca gctccttgcg
     4081 catgctatcc aaatatcaaa ccccatgtta tgcgcccgcc cgctatgacg aactataaat
     4141 ccgacctgct gcgccttctc gacgagcgag gctatatcca tcagacgacc gacgcggaag
     4201 ggctcgatgc gctcgcggcg aaacaggtcg tccccggata tatcgggttc gacgcaaccg
     4261 cgccgtcgct gcacgtcggc agcctcgtcc agatcatgat gctgcgccgc ctgcaacaga
     4321 cggggcacaa gcccgtcgtg ctgatgggcg gcgggacgac gcggatcggc gacccgaccg
     4381 ggcgcgacga aagccgcaag atgctgtcgg acgaaacgat cgccgcgaac atcgcgtcga
     4441 tcttcagcat cttccagcaa ttcctcagct ttggcgacgg cccgaccgac gcggtgatgg
     4501 tcgataatca ggactggctc ggccagctcg gctatatcga gctgctgcag gaagtcggca
     4561 cccacttcac gatcaaccgc atgatgacct ttgattcggt caaactgcgg ctcgagcgcg
     4621 aacagccgat gaccttcctc gaattcaact acatgatcct ccagggctat gatttccgcc
     4681 atctgtccaa gaataggggc gtccggctgc agatgggcgg atcggatcag tggggcaata
     4741 tcgtcaacgg gatggagctt ggccgccgga tggacggcgc cgaccttttc ggcctgacga
     4801 ctccgctgct gaccaccgcc gcaggcgcca agatgggcaa aaccgccgcc ggcgcggtgt
     4861 ggctcaatcc cgaacaactg tcccattttg actattggca atattggcgc aactgcgacg
     4921 accgcgacgt cggcaagttc ctcaagctgt tcaccgacct gccgctcgag gagatcgcgc
     4981 ggctcgaagc gctcgaaggc gccgagatca acgaggcgaa gaaaatcctc gccaacgagg
     5041 cgaccgcgat gtgccgcagc caagaggccg caaaggccgc ttcggacacc gccgcgcaaa
     5101 cctttgaaaa gggacagatc ggtggcgacc ttccgcaagc cgtcgcgccc gccgaaggga
     5161 tgagcgtcgt cgatgcgctg cgcgaactcg gctttgccgc gtcgaacaag gaggcccgcc
     5221 gcaagctcga cgagggcgcg gtcaaggtcg acgggacggt cgttcgcgac ccgcaacatt
     5281 tgatccagcc gggcgagacg ccggttccgc tgagcctcgg atcaaaaaaa catggccttg
     5341 tgacgcggta gggcgcagaa aagcgtttac cacttcttca gcaatcggat ggatcgtcat
     5401 ggcaaaatca acaaggtcca cgaacatgcg caagatcgac accagcaagg cccattatgt
     5461 cgggctcgac cagcggatcg ctccgcgtag cgacgtctat tgccgcctgc ccttcgtcat
     5521 gcccgatggc cggcaggaaa tgtgcacctg cgtcaacatc agcgccgacg gcctgctgat
     5581 gcgcttcgag cgcggactgg agcccggcga cctcgtcgtg ttccgcatgc cgatcatcgg
     5641 ccgcaccgcg gcgaaagtcg tctggtcatt gggcggcaag acgggtgtgc agttcgacaa
     5701 atcgatctcg gtcgaggatt atctgccgat gatccgcgcc atgggcgcgc gcggggatgt
     5761 gaactaagaa acattgcggg cagcggcacg ctcttctccc ctcccgcttg cgggaggggg
     5821 cgggggaggg tctgtctatg tgaactaccg atagggagga acatgccctc ccctaacccc
     5881 tcccgcaagc gggaggggaa ctattatcca ctcctgagca acggcaccgc cgcatcgcgc
     5941 tcaaacagat agaggcagag ccgcaccgcc tcgccgcggg cgccctccat gccgccatcg
     6001 cgctcgacga agagccgggc atcatcatgc gcgacgggta gcaagcgcac gagctgttcg
     6061 ggggtcgcga gccggtagtc ggcgtcgccc gactgcttga gcccgagcag ttcgccgccg
     6121 ccgcgcagtt ccagatcctt ctcggcgatg acgaagccgt cgttggtctc gcgcatcagc
     6181 gcgagccgct cgcgcgcggt ttccgacaga ttctgcgacc gcagcagcag gcataccgac
     6241 ttcgccgccc cgcgtccgac ccggccgcgc agctggtgaa gctgcgccag cccgaaattt
     6301 tcggcatgtt cgacgatcat caggctggcg gcgggcacgt cgacgccgac ctcgatcacc
     6361 gtcgtcgcga cgagcacgcc gatctcgccc gcctggaagc gcgccatgac ctcgtccttt
     6421 tcgggaccct tcatccggcc atgcacgagc ccgacgcgcg cgtcgcccag ccgctcgcgc
     6481 agcagcgcgg cgcggtcttc cgccgccgcg agttcgctcc cctcgctctc ggcgaccagc
     6541 ggacacaccc aataggcctg cgcgcccgtc gccagatgcc ggtcgagcga gcccacgaca
     6601 tcgtcgagcc ggtcgagcga gatgacgcgc gtatcgaccg gggtgcgccc cggcggcatt
     6661 tcgtcgattt gcgacacgtc catctcgcca tgattggcga gttgcagcgt gcgcgggatc
     6721 ggggtcgcgg tcatcacgag gaggtgcggc gggcgcgcgg ccttttgcgt cagcatcaat
     6781 cgctgcgcga caccgaagcg gtgctgttcg tcgacgacga cgagcgcgag atccttgtac
     6841 gtcaccgcct gctggaagat cgcgtgggtg ccgacgagga tgtcgatgct gccgtcggtg
     6901 aggcccatca atgtcgattc gcggactttg cccttgtcgc gcccggtcag gatcgcaagg
     6961 ttgacaggca aaccgccaag catccgctgc aaggtcgcaa aatgctggcg cgccaaaatc
     7021 tcggtcgggg cgagcagcgc cgcctgcgtc cccgcctcga ccgccgacag catcgcgcgc
     7081 aaggcgacga gcgtcttgcc cgatccgaca tcgccttgca gcatgcgcag catcggcgtg
     7141 tcctgcgcca t
//
LOCUS       DATMSU010000173         2866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_12581, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000173 DATMSU010000000
VERSION     DATMSU010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12581"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1929
                     /gene="infB"
                     /locus_tag="VN034_04450"
     CDS             <1..1929
                     /gene="infB"
                     /locus_tag="VN034_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWV59879.1"
                     /translation="EPKATRGGDSRRQSGKLTVTRALNEDEGARARSLAALKRAREKE
                     KRSHTASSGPREKQIREVTVPESITVQDLANRMAEKTSDLVKALFKMGVPSASTDTID
                     QDTAELLVTEFGHEIIRVSEGDIDIRHDEDVDDAEHLKPRAPVVTIMGHVDHGKTSLL
                     DALRGANVQAGEAGGITQHIGAYQVKTPDGSLVTFLDTPGHEAFTEMRQRGANVTDIV
                     ILVVAADDGLKPQSIEAINHAKAANVPIIVAINKVDKEGANPQRVRERLLEHELVVEE
                     MGGDVQNVEVSALKKTGLDKLLDAIAVQAEIMELKANPDRAAEGTVVEAKLDKGRGPV
                     ATILVRRGTLKVGDIFVCGAESGRVRALIDDQGKQIKSATPSMPVEVLGLGGVPMAGD
                     TLTVVENEARAREVATYRQEQATRKRTVQAPVSLEGMFEALADKANVIQFPVIIKGDV
                     QGSVEAIVNALNKLSTDEIRVRVLQSGAGAITESDVTLAAATKAPIIGFNVRPNAKAR
                     DIAKREKVRFMYHDVIYHLTDEIRKEMAGELGPERIETVVGRAEVKDVFPAGKRDKAA
                     GLLVLEGSIRKGLHARLTRDDVIVSATTISSLRRFKDDVAEVRAGLECGVVLADTNDI
                     KAGDHLEVFEVELRERTL"
     gene            1966..2517
                     /locus_tag="VN034_04455"
     CDS             1966..2517
                     /locus_tag="VN034_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWV59880.1"
                     /translation="MARYVALFGSINVGGNRLTMADLRSAFEAEGFSNVETVVASGNV
                     LFDHDARPTRGLEEKLSIMVDERFYMTSAALVRDRDELAAAIADNPFAGTNEDRIVHT
                     MFLDGQPTAEQFDALATDHRVRPNERLALGDRALYIDFGDGAADSKLHARLIERRLGH
                     KGTARNMRSIARIIAKLDEEAKG"
     gene            2521..>2866
                     /gene="rbfA"
                     /locus_tag="VN034_04460"
     CDS             2521..>2866
                     /gene="rbfA"
                     /locus_tag="VN034_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWV59881.1"
                     /translation="MKTEKEKGPSVRVLRVGEQVRHLLSEILARGDAHDELLAKHPVS
                     ITEVRMSPDLRHATVFVKPLLGKSEEAVLKALRTNTAWLQKSVAEKMSMKYAAKLKFL
                     SDESFDEASHIEK"
BASE COUNT          597 a          910 c          944 g          415 t
ORIGIN      
        1 gagccgaagg caacccgcgg cggcgacagc cgccgccagt cggggaaact caccgtcacg
       61 cgggcgctga acgaagacga aggtgcgcgc gcgcgcagcc tcgcggcgct gaagcgcgcc
      121 cgcgaaaagg aaaagcgttc gcacaccgcc tcgtcgggtc cgcgtgagaa gcagatccgc
      181 gaagtcaccg tgcccgaaag catcacggtt caggatctcg ccaaccgcat ggccgaaaag
      241 accagcgatc tggtcaaggc gctgttcaag atgggcgtcc cgtcggccag caccgacacg
      301 atcgatcagg acaccgccga actgctcgtc accgagttcg ggcacgagat catccgcgtc
      361 agcgaaggcg acatcgacat ccgtcacgat gaagatgtcg acgatgccga acatctgaag
      421 ccgcgtgccc cggtcgtcac gatcatgggt cacgtcgacc acggcaagac gtcgctgctc
      481 gacgcgctgc gcggtgcgaa tgtgcaggcg ggcgaagccg gcggcatcac ccagcatatc
      541 ggcgcctatc aggtgaagac ccccgacggg tcgctcgtca ccttcctcga tacgccgggc
      601 cacgaagcct ttaccgagat gcgtcagcgc ggtgccaatg tcaccgacat cgtcatcttg
      661 gtggtcgccg ccgacgacgg cctcaagccg cagtcgatcg aggcgatcaa tcatgcgaag
      721 gcggcgaacg tgccgatcat cgtcgcgatc aacaaggtcg acaaggaagg cgccaatccg
      781 cagcgcgtcc gcgaacgcct gctcgagcat gagctggtgg tcgaggaaat gggcggcgac
      841 gtgcagaatg tcgaagtgtc ggcgctcaag aagaccggcc tcgacaaatt gctcgacgcg
      901 atcgcggtgc aggccgaaat catggagctc aaagccaacc ccgaccgcgc cgccgaaggc
      961 acggtggtcg aggcgaagct cgacaagggc cgcggccccg tcgcgacgat cctcgtccgc
     1021 cgcggtacgc tcaaggtcgg cgacatcttc gtctgcggcg ccgaaagcgg ccgtgttcgc
     1081 gcgctgatcg acgaccaggg caagcagatc aaatcggcga ccccgtcgat gccggtcgaa
     1141 gtgctcggtc tgggcggcgt cccgatggcg ggcgatacgc tcaccgtcgt cgagaatgaa
     1201 gcgcgcgccc gcgaagtcgc gacctatcgt caggaacagg cgacgcgcaa gcgcaccgtc
     1261 caggcaccgg tcagcctcga aggcatgttc gaggcgctcg ccgacaaggc gaacgtcatc
     1321 cagttcccgg tgatcatcaa gggcgatgtg caggggtcgg tcgaggcgat cgtcaatgcg
     1381 ctgaacaagc tgtcgaccga cgagatccgc gtccgcgtgc tccagtcggg cgccggcgcg
     1441 atcaccgaga gcgacgtgac gctggcggcg gcgaccaagg cgccgatcat cggcttcaac
     1501 gtccgtccga acgccaaggc gcgcgatatc gcgaagcgcg aaaaagtgcg cttcatgtat
     1561 catgacgtca tctatcacct gaccgatgag atcaggaagg aaatggcggg cgagctgggt
     1621 cccgaacgga tcgaaaccgt cgtcggccgc gccgaggtca aggacgtgtt ccccgcgggc
     1681 aagcgcgaca aggctgcggg tctgctcgtg ctcgaaggct cgatccgcaa ggggctgcac
     1741 gcacgcctta cgcgcgacga tgtcatcgtg tcggcaacga ccatctcgtc gctccgccgc
     1801 ttcaaggacg acgtcgccga agtgcgcgcg ggtctcgaat gcggtgtcgt gctggcggat
     1861 accaacgaca tcaaggcggg cgaccacctc gaagtcttcg aagtcgaact gcgcgaacgg
     1921 acgctctaac gggcgtccgc tccgcgcggg tcaggaggtg ccggcatggc tcgctatgtg
     1981 gcattgttcg gcagcatcaa cgtcggtggc aaccggctga cgatggccga cctgcgttcg
     2041 gcgttcgagg ccgagggttt cagcaacgtc gaaaccgtcg tggcgagcgg caatgtgctg
     2101 ttcgaccatg acgcgcggcc gacgcgcggg ctcgaagaga aattgtcgat catggtcgac
     2161 gagcgtttct acatgacgag cgcggcgctg gtgcgcgatc gcgacgaact cgccgcggcg
     2221 atcgccgaca atccgttcgc gggcacgaac gaggatcgca tcgtccatac gatgttcctc
     2281 gacggtcagc ccaccgcgga gcagttcgat gcgctggcga ccgaccaccg cgtccggccg
     2341 aacgagcgcc tcgcgctcgg cgaccgggcg ctctatatcg actttggcga cggagccgcc
     2401 gacagcaagt tgcacgcgcg gctgatcgag cggcggctgg gacacaaggg caccgcgcgc
     2461 aacatgcgct cgatcgcgcg gatcatcgcg aaactggacg aagaggcaaa gggttaggcg
     2521 atgaaaaccg aaaaagaaaa aggcccctcg gtccgcgtgc tacgcgtcgg cgaacaggtg
     2581 cgccacctcc tctccgaaat cctcgcacgc ggcgacgcgc atgacgagct cttggccaag
     2641 catccggtga gcatcaccga ggtccgcatg tcgcccgacc tgcgccacgc aacggtgttc
     2701 gtgaaaccct tgctcggcaa gagtgaggaa gcggtgctga aggcgctgcg caccaacacc
     2761 gcctggctgc aaaagagcgt cgcggagaag atgagcatga aatatgccgc gaaactgaaa
     2821 ttcctgtcgg acgaaagctt cgacgaggcg agccatatcg aaaaat
//
LOCUS       DATMSU010000174         7349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_183301,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000174 DATMSU010000000
VERSION     DATMSU010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183301"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1033)
                     /locus_tag="VN034_04465"
     CDS             complement(56..1033)
                     /locus_tag="VN034_04465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HWV59882.1"
                     /translation="MTANADIIEGLRAAGLVGAGDVVLEPLTGGVSCDVWKVETPSGP
                     IVVKRPLPQLRVAAEWHAPVERGTSEVRWLRRARGVDARIAPEVLAELPGHAFAMRFL
                     PGCPVWKDELMAGRVDSDFAAQVGTRIAAVHAATAHKGGDRADFPNDEMFRALRVDPF
                     LLHVARHDAELAPALTALADDLAARKIALVHGDVSPKNILVSADGPVFLDAECAVYGD
                     PAFDLAFCTTHLLLKAVWSGDARMNEAAIALVAAYRAGIDWEDAGGLLLRAGKLTAAL
                     LLARVEGKSPAPYLTDPEHKRIVRDQARTLIVAPSPIDVLVANWKRNLV"
     gene            complement(1035..2162)
                     /locus_tag="VN034_04470"
     CDS             complement(1035..2162)
                     /locus_tag="VN034_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59883.1"
                     /translation="MDPNGIEGLDAQFIGRVSPTAPRIQAGGHPCQGIYWTEAGKRPK
                     IAIIATHYNVDFSEHYIAPYFARHGFGFLGWNTRYRGVEDQFLLEHAVLDIGVGMKWL
                     KEEAGVEQIVILGNSGGGSLMGAYQAEAIAPTLTDRLPSVGQDALAQLIKGDLYISFN
                     AHQGRPEVLTDWMDASVIDENDPVATDPELDPFNPDNGPPYSDEFIAKYRAAQRARNQ
                     RITDWAKAELKRLNDAGIPDRIFPMFRCWGDIRCVDPAIDPSDRKPNWCYRGDPAIAN
                     RTPSIGRANTIKTWLNMWSLETSPCQGQPHLAKHDTPALVVQGTADTGVFPSDARKIF
                     DFLGSGDKKLELIPGAHYFEDSIEERQNAADLVGAWIREKL"
     gene            complement(2190..3824)
                     /locus_tag="VN034_04475"
     CDS             complement(2190..3824)
                     /locus_tag="VN034_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HWV59884.1"
                     /translation="MDGLMQNVPLTVDRIIDHAANWHGAREIVSRDAEGRVTRSTYAD
                     IHADAKRVSNALAAEGIEPGDRVATMAWNGSRHLAAWYGAAGMGAVLHTLNPRLFLEQ
                     IAYIANHAGDRLLIADPATADLVEALLPQVPSIEKVIFFCDRASLPETSFDAVAFDDW
                     IEGHSAEYDWGGFDENAACGLCYTSGTTGNPKGVLYSHRSNYIHALMTLQRDALALSA
                     RDTVLLVVPMYHANAWGVVYSAPAVGAKLVLPGQRMDGESIYNLIEQEGVTYSAAVPT
                     VWQMLLTYMQENAKRFTTLERVTIGGSACPESIIRTFRDDYGVDVIQGWGMTETSPLG
                     TVSVPSASVAAKSEDEQMAYKLKQGRLLCGLEMKLVDDAGNRLPHDGKTPGRLMVKGA
                     TIAGAYYGGEGGDVLDDEGFFDTGDVSTIDGEGYMQITDRAKDVVKSGGEWISSIEIE
                     NIAMGHDAVANAAVVGIAHPKWDERPILLCQLKDGATACAEDLKAYLDGKIARWWMPD
                     DVLFVDKIPLGPTGKIDKKAIRAGLEGYKLPFEVTR"
     gene            3969..4418
                     /locus_tag="VN034_04480"
     CDS             3969..4418
                     /locus_tag="VN034_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWV59885.1"
                     /translation="MRTNQLIIALFQRFCWLDEGLQARLHDHGWPDVNRPQSMIMTNI
                     VSGVVRPSDIARNLGVSRQAVHSTINQMVKLGIVRLDVDPDDRRHMIVSLTELGARMR
                     KDAQRSMDALTAQIADRLGQDKFDALLAALEADWGNNIDRPVAAPRR"
     gene            complement(4415..4990)
                     /locus_tag="VN034_04485"
     CDS             complement(4415..4990)
                     /locus_tag="VN034_04485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWV59886.1"
                     /translation="MIKIAVIVGSTREGSYNRALGELAAASLEAQGASVTRVNLAAFD
                     LPLYSAALEAGAFPPDALKLKTLFAAQDGLLFVSPEYNGSLSPLLKNAIDWASRPTGD
                     ESLVALSAYRGKAAAIMSASISPFGGLRGLMHLRQILSTIQMLVIPEQVLVPNAHAAF
                     AEDGSLKEPLPASLVEMTAARLVAVAKALSA"
     gene            complement(5081..5560)
                     /locus_tag="VN034_04490"
     CDS             complement(5081..5560)
                     /locus_tag="VN034_04490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HWV59887.1"
                     /translation="MTITARMNRLTFNDADEVRALFRELTGQKVDESFLLIPPFYTAH
                     GTEIRVGRNVFVNQNCTFYDLGGLDIADDVLIGPNVSLITTGHPLEPSQRRAVTIGKP
                     IVIERNVWIATGVTIIGGVTIGENSVVAAGSVVTRDVPPNSLAAGNPARVVRAIGDG"
     gene            complement(5677..6183)
                     /locus_tag="VN034_04495"
     CDS             complement(5677..6183)
                     /locus_tag="VN034_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWV59888.1"
                     /translation="MVKKTKDYRHPAEYYTDPENSIGYLARVVFRSFSRLLERRTLTH
                     DVSAGQWRFLRQLWREDGITQRELSERVGMREPTTVVALKGLEKAGLITRKKTADDRR
                     KTFIHLTPHAKKLELILAPMNAEIHEIATKGMSDAEVEVLQGLMRRVIDNLADETRKL
                     AVLSDIKA"
     gene            6369..6953
                     /gene="leuD"
                     /locus_tag="VN034_04500"
     CDS             6369..6953
                     /gene="leuD"
                     /locus_tag="VN034_04500"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWV59889.1"
                     /translation="MQKFIRVKAAAAPLPLTNVDTDMIIPAEYMKALTRSGLGRHLFR
                     ALRFDTDGRERADFILNRPACRDARILVADRNFGCGSSREHAVWALTDFGIRCVIAPS
                     FGDIFAGNARKNGLLLIRLPDERCARLRAEIEAAQYAPVEVDLEAQRIRLASGETIAF
                     DLDPGDRRILMEGLDDIDRTLRHADAIARFEAAT"
     gene            complement(6950..>7349)
                     /locus_tag="VN034_04505"
     CDS             complement(6950..>7349)
                     /locus_tag="VN034_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWV59890.1"
                     /translation="AGQRLDRIFIGSCTNSRIEDLRVAADIVRGRHVAPHVRAMVVPG
                     SGLVKRQAEAEGIADTLRAAGFDWREPGCSMCVGMNADRLNPGERCAATSNRNFENRQ
                     GRGGRTHLMSPALAAASAVAGCIASPDMLD"
BASE COUNT         1263 a         2456 c         2270 g         1360 t
ORIGIN      
        1 ggccgaccgc gcgaatccca gatctggcgg ccggtgacag aggcgatacg cgaagtcata
       61 caaggttcct tttccagttg gcgacgagca cgtcgatcgg cgacggcgcg acaatgagtg
      121 tccgtgcctg atcgcgcacg atgcgcttgt gttcggggtc ggtgagatag ggcgcgggcg
      181 acttgccctc gacgcgcgcg aggagcagcg cggcggtgag cttgccggcg cgcagcagca
      241 agccgccggc gtcttcccag tcgattccgg cacggtaggc ggcgaccaag gcgatagcgg
      301 cttcattcat ccgggcatcg cccgaccaca ccgctttcag cagcaggtgc gtcgtgcaaa
      361 aggcgaggtc gaaggccgga tcgccataga ccgcgcattc ggcgtcgaga aacaccgggc
      421 catcggcgct gacgaggata ttcttcgggc tgacgtcgcc atggacaagc gcaatcttgc
      481 gcgccgccag atcgtcggcg agcgcggtca gcgcgggcgc aagttcggcg tcgtgccgcg
      541 cgacatggag caggaaggga tcgacgcgca gcgcgcggaa catctcgtcg ttggggaaat
      601 cggcgcggtc gccgcccttg tgcgcggtcg cggcgtggac cgcggcgatg cgcgtcccca
      661 cctgtgcagc aaaatcgcta tcgacacgcc ccgccatcag ttcgtccttc cacaccgggc
      721 aaccgggcag gaaacgcatc gcgaaggcgt gaccgggcag ttcggcgagc acttcgggtg
      781 cgatgcgcgc gtccacgccc cgcgcccgcc gcagccagcg cacctcgctc gtcccgcgct
      841 cgaccggcgc atgccattcg gcggcgacac gaagctgcgg cagcgggcgc ttgacgacga
      901 tcgggcccga cggcgtctcg accttccaca cgtcgcacga cacgccgccg gtcagcggtt
      961 cgagaacgac atcgcccgca cccaccagcc cggcggcgcg caatccctcg atgatatcgg
     1021 cattcgccgt cacgtcagag cttctccctg atccatgcgc cgacgaggtc ggcggcattc
     1081 tgccgctcct cgatcgagtc ctcgaaataa tgcgcgccgg ggatcaattc gagcttcttg
     1141 tcgccgctcc ccaggaaatc gaagatcttg cgcgcgtcgc tcgggaagac gccggtgtcg
     1201 gcggtgccct gaacaacgag cgcgggggtg tcgtgcttgg cgaggtgcgg ctgcccttgg
     1261 cacggcgagg tttcgaggct ccacatgttg agccaggtct tgatcgtgtt ggcgcgcccg
     1321 atgctcggcg tgcggttcgc gatcgcggga tcgccgcgat agcaccaatt gggcttgcgg
     1381 tccgacgggt cgatcgccgg atcgacgcag cggatatcgc cccagcagcg gaacatcggg
     1441 aagatacggt cggggatgcc cgcatcgttg agccgcttca actcggcttt cgcccaatcg
     1501 gtgatccgct ggttgcgcgc gcgctgcgcg gcacgatatt tggcgatgaa ttcgtccgaa
     1561 tagggcggac cgttgtcggg gttgaacggg tcgagttcgg ggtcggtcgc cacggggtca
     1621 ttctcgtcga tcaccgacgc gtccatccag tcggtcagca cttcgggacg cccctgatgc
     1681 gcgttgaagc tgatgtagag gtcgcctttg atgagctgcg ccagcgcgtc ctgccccacc
     1741 gaaggcaagc gatcggtcag cgtcggcgcg atcgcctcgg cctgataggc gcccatcagc
     1801 gacccgccgc ccgaattgcc gaggatgacg atctgttcga ccccagcctc ttccttcagc
     1861 cacttcatgc cgacgccgat gtcgagcacc gcatgttcga gcaggaactg gtcctcgacc
     1921 ccgcgatagc gcgtattcca gccaaggaag ccgaagccat gccgcgcgaa atagggtgcg
     1981 atataatgtt cggagaaatc gacgttgtaa tgcgtcgcga tgatcgcgat cttcggccgc
     2041 ttgcccgcct cggtccaata aatgccctga cacggatgcc cgcccgcctg aatgcgcggc
     2101 gcggtcggcg acacgcggcc gataaattgc gcgtccaatc cttcgatccc gttcggatcc
     2161 attagtctct ctcctgcttg tcgattgttt cagcgggtca cttcaaaagg cagtttatat
     2221 ccctccagcc ccgcgcggat cgccttcttg tcgatcttgc ccgtcgggcc gagcgggatt
     2281 ttgtcgacga acagcacgtc gtcgggcatc caccagcgcg cgatcttgcc gtcgagatag
     2341 gccttcaaat cctcggcgca ggcggtcgcg ccatccttga gctggcacag caggatcggc
     2401 cgctcgtccc acttcggatg cgcgatcccg acaaccgcgg cgttggcgac ggcatcatgc
     2461 cccatcgcga tattctcgat ctcgatcgaa ctgatccact cgccgcccga cttcacgacg
     2521 tccttggcgc gatcggtgat ctgcatatag ccttcgccat cgatcgtgct gacgtcgccg
     2581 gtatcgaaaa agccctcatc gtcgagtacg tcaccgcctt cgccgccata ataggcgccc
     2641 gcgatcgtcg cccccttaac catcagccgc cccggcgtct tgccgtcgtg cggcaaccgg
     2701 ttgccggcat cgtcgacgag cttcatctcg agcccgcaga gcagccgccc ttgcttgagc
     2761 ttatacgcca tctgctcgtc ttcggacttg gccgcgaccg atgcgctcgg caccgacacg
     2821 gtgccgagcg gcgaggtttc ggtcatgccc cagccctgga tgacgtcgac gccataatcg
     2881 tcgcggaagg tgcggatgat cgattcaggg cacgccgagc cgccgatcgt cacgcgctcc
     2941 agcgtggtga aacgctttgc attttcctgc atataggtga gcagcatctg ccacaccgtc
     3001 ggcaccgcgg ccgaataggt cacgccttcc tgctcgatca gattatagat cgattcgccg
     3061 tccatccgct gccccggcag gacgagcttc gcgccgaccg ccggtgccga atagacgacg
     3121 ccccacgcat tggcgtgata catcggcacc acgagcagca ccgtatcgcg cgccgacaat
     3181 gcgagcgcgt cgcgctgcaa ggtcatcagc gcgtggatat aattcgagcg gtgcgaatag
     3241 agcaccccct tcggattgcc cgtcgtgccg ctggtgtagc agagcccgca cgcggcattc
     3301 tcgtcgaagc cgccccagtc atattcggcc gaatgcccct cgatccagtc gtcgaacgcg
     3361 accgcatcga agctcgtttc ggggagcgac gcgcggtcgc agaaaaagat caccttctcg
     3421 atcgacggca cttgcggcag cagcgcttcg acgagatcgg cggtcgcggg atcggcgatc
     3481 agcagccggt cacccgcatg gttcgcgata tacgcgatct gttcgaggaa cagccgcggg
     3541 ttgagcgtat ggagcaccgc ccccatcccc gccgcgccat accaggcggc aaggtgccgc
     3601 gatccgttcc acgccatcgt cgcgacgcgg tcacccggct caatcccctc ggcggcgagc
     3661 gcgttcgaca cgcgcttcgc gtcggcatgg atgtcggcat aggtcgagcg ggtgacgcgt
     3721 ccctcggcat cgcgcgacac gatctcgcgc gcaccatgcc agttcgccgc atggtcgatg
     3781 atccggtcga ccgtcagcgg cacattctgc atcaacccgt ccatgcggtc ctctccctcg
     3841 tcgttgaata gatagtatac taagtatttt gcctttgcaa cgccgcccgg ccttgcttcg
     3901 cccgcatcgc tcttctagcc tgacgatgtc ggcaccccgc cggcaacagg ggcacaacat
     3961 agcgtcatat gcgcacaaat cagcttatca tcgcgctttt tcaacgcttc tgctggctcg
     4021 acgaagggct gcaggcgcgg cttcacgatc atggctggcc cgacgtcaat cgcccgcaat
     4081 cgatgatcat gaccaatatc gtcagcggcg tcgtgcgccc gtcggacatc gcacgcaatc
     4141 tcggcgtgtc gcggcaggcg gtccacagca cgatcaacca gatggtgaag ctcggcatcg
     4201 ttcggctcga cgtcgatccc gacgatcgcc gccacatgat cgtgtcgctg accgaacttg
     4261 gcgcgcgtat gcgcaaggac gcgcagcggt cgatggacgc gctcaccgcg cagatcgccg
     4321 accggctggg gcaggacaag ttcgatgcgc tgctcgcggc gctcgaggcc gactggggca
     4381 ataatatcga ccgccccgtc gccgccccgc gccgctaggc cgacagggcc ttcgcgactg
     4441 cgaccagccg cgcggcggtc atctcgacca gcgatgcggg caacggctcc ttcagactgc
     4501 cgtcctcggc aaaggccgcg tgcgcgttcg gcaccagcac ctgctcgggg atcacgagca
     4561 tctggatcgt cgacaagatc tggcgcaggt gcatcagccc gcgcagcccc ccgaatgggc
     4621 tgatcgacgc cgacatgatc gccgccgcct tgccgcgata ggcggagagc gcgacaaggc
     4681 tctcgtcgcc cgtcggccgc gacgcccagt cgatcgcatt tttgagcagc ggcgacagcg
     4741 aaccattata ttcgggggac acgaacagca ggccgtcttg cgcggcgaac agcgtcttga
     4801 gcttcagcgc gtcgggcggg aaggcaccgg cttcgagcgc cgccgaatag agcggcaggt
     4861 cgaacgccgc gaggttgacc cgcgtgacgc ttgcgccctg cgcctcgagg ctcgcggcgg
     4921 cgagctcgcc cagagcgcga ttataggatc cctcgcgcgt gcttccgacg attaccgcaa
     4981 tctttatcat cattcctccc gaaccagatc attagaatac taacttataa ttcgagcgaa
     5041 acgcaactcc gcatcacgac cggacacctt tcggagaatc tcaaccgtcg ccgattgcac
     5101 ggacgacccg cgctggattt cccgcggcaa ggctgttcgg ggggacgtcc ctggtcacaa
     5161 ccgaaccggc cgctaccacc gaattttctc cgatcgtcac gccgccgatg atcgtcacgc
     5221 cggttgcgat ccagacgttt ctttcaataa caatcggctt tccgatcgtc acagcccggc
     5281 gctgcgacgg ctccagcgga tggccggtcg tgatcaggct gacattcggc ccgatcagca
     5341 cgtcgtcggc gatgtccagc ccgccgagat cgtagaaagt gcagttctga ttgacgaata
     5401 cattgcgccc gacgcgaatt tcggttccat gtgccgtgta gaagggcggg atcaacagaa
     5461 agctttcatc gaccttttgg ccggtaagtt cgcggaagag cgcccgaacc tcatccgcgt
     5521 cgttgaatgt cagccgattc attcgggcgg tgatcgtcat cgctcgtttc gtattcgtga
     5581 ccatcgccgc cgattccggc gtccgcccgt ggattataat gatgcgttcc gcgtccggca
     5641 tttcgagttt ttccgggcca gcaacctgtt cggcgatcag gccttgatgt ccgaaagcac
     5701 cgcgagcttg cgcgtttcgt cggcgagatt gtcgatcacg cggcgcatca gcccctgcag
     5761 cacctcgacc tccgcatcgc tcataccctt ggtcgcgatc tcgtggatct cggcgttcat
     5821 cggcgcgagg atcaactcga gcttcttcgc gtgcggggtc agatggatga aggtcttgcg
     5881 ccggtcgtcg gcggtcttct tgcgtgtgat cagccccgcc ttttccagcc ctttcagcgc
     5941 gacgacggtg gtcggctcgc gcatcccgac gcgttcgctc agttcgcgct gggtgatgcc
     6001 gtcttcgcgc cacagctgac gcaggaaacg ccactgcccc gccgaaacat cgtgcgtcag
     6061 cgtgcggcgt tcgagcaacc gcgaaaagga gcggaagacg acgcgcgcga ggtagccgat
     6121 gctgttttca ggatcggtat aatattcggc cggatgccga taatcctttg ttttcttaac
     6181 caaaatcctg ctcccctgtc gcagcgccag gtgctcgccc cgcgccgggt tgaatcttag
     6241 gacgcgaagg ttcgcgcggc aaccatcttc atcttcgctt cccgccccgt cgccgttcca
     6301 accccgcgat aatgttggac aagattcgcc ggtcgatata cttagcatac taagttaatg
     6361 gaggcgatgt gcaaaaattc atccgggtga aagcggccgc ggcgcccttg ccgctcacca
     6421 atgtcgacac cgacatgatc attcctgccg aatatatgaa ggcgctgacg cgttcgggcc
     6481 ttggccgcca tctgttccgc gcgttgcgat tcgatacgga cggccgcgag cgcgcggatt
     6541 tcatcctcaa ccggcccgcc tgccgcgatg cgcggattct ggtcgccgac cgcaatttcg
     6601 gctgcggctc gtcgcgcgaa catgcggtgt gggcgctcac cgacttcggt atccgctgcg
     6661 tcatcgcgcc gagtttcggc gatatcttcg cgggcaatgc gcgcaagaac gggctgctgc
     6721 tgattcgctt gcccgacgaa cgctgcgccc ggcttcgcgc ggagatcgaa gccgcgcaat
     6781 atgcgccggt cgaggtcgac ctcgaagcgc agcgcatccg cctcgcctcg ggcgagacga
     6841 tcgctttcga cctcgatccg ggcgaccggc gcatcttgat ggaagggctc gacgatatcg
     6901 accgcacgct gcgccacgcc gatgcgatcg cgcggttcga ggcggcgacc tagtcgagca
     6961 tatcgggcga cgcgatacat cccgcgaccg cgctcgccgc cgcgagtgcc gggctcatca
     7021 ggtgggtgcg tccgccgcgt ccctggcgat tctcgaaatt gcggttcgag gtcgcggcgc
     7081 agcgttcgcc ggggttgagc cggtcggcat tcatgccgac gcacatcgag cagccgggct
     7141 cgcgccagtc gaaccccgcg gcgcgcagcg tgtcggcgat tccctccgcc tcggcctgtc
     7201 ttttgaccaa ccccgatccc ggcacgacca tcgcgcggac gtgcggcgcg acatggcggc
     7261 cgcgcacgat gtcggcggcg acgcgcaaat cctcgatccg gctgttggtg cagctgccga
     7321 tgaagatgcg gtcgagccgc tgtccggcg
//
LOCUS       DATMSU010000175         4808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_154861,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000175 DATMSU010000000
VERSION     DATMSU010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154861"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VN034_04510"
     CDS             complement(<1..584)
                     /locus_tag="VN034_04510"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWV59891.1"
                     /translation="MPATPAWPPASTPRLFIDQPLGLDAAPVLDGPAAHYLLGVMRLK
                     AGDPVLLFDNRTGEWLAVVADAAKRSATLRIERQTRPMERVPDLWLCFAPVKKARLDW
                     IIEKATELGVARLQPVITERTIVERVKSERIEAQIVEACEQCGRTALPELAPPVKLPQ
                     LLKDWPTERALLFADEAGGVPLAEVAAPAPAAILT"
     gene            787..2778
                     /locus_tag="VN034_04515"
     CDS             787..2778
                     /locus_tag="VN034_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HWV59892.1"
                     /translation="MRGTWISRLRAGAKELLRDQRGNAFMLTAAAIVPVIGIVGSAVD
                     IGRAYMTQLRLQQACDAGVLAGRRAMAGGTYTDDAEAEANKMFNFNFPEGKYGASGIL
                     FSSEADEGGSDVLGQASAVLPTELMFMFGKDEFRLSANCTAKLEISNVDVMLVLDVTG
                     SMERTNSGDSVDRITALQDATMDFFDTLTTADVGDGRLRFGVVPYSSTANVGAILLEE
                     NPEWISDFTILPSRSPVMRYDWSGTNPPSSVTSGTTTNSAWEDFLPISGFTSSAACSA
                     VVAPGDTMPALSTEQDMNRTGWAVRSNGNRIFATVAGTRHRYFNYRYQYNSSNSTCWL
                     QRRTVTFDHAASPSPTSGSPSFNSQYRYEDRVFDVSNMKAGGTITVNTGNSGGNRTLS
                     WSGCVIERRTSPFGASSSAPSSALDMDIDLVPEDENSRWRILLPELSYHRATNLNTVP
                     SGTGVRTVNSSNVSGGNWQSFEYHWPNGWGVCPAAAMRLTTMTAEDRSAFNSYVQSLR
                     PVGGTYHDAGMIWGARLLSPSGLFADENATAPNNRPISRHIVFMTDGEMAPNWQNLTF
                     QGYEYLMQRISGSATTDNSLLTNRHNNRFVQLCRAARQRGITIWVVSFGDDSNSAENL
                     ANLNSCASSGTAFEADDAAELNEQFQAIARQISKLRLSQ"
     gene            2775..3344
                     /locus_tag="VN034_04520"
     CDS             2775..3344
                     /locus_tag="VN034_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HWV59893.1"
                     /translation="MTGVRPLLRRLRRSERGTAFVEFALTAPVFLLILLGIFDYCWQM
                     YAQQVLQGVVAKAGRDATLEGFAADQAKLDKAVETQVKKVFKSAEVDFNRRAFDDYSE
                     IKPLRWVDTNGNGVQDPSPDDCWEDGGKQGNGGADDVVQYTVSMQFDRVLPVWKMLGQ
                     PQSMTLTSTTLLRNQPFAAGGEVLVETCG"
     gene            3341..4003
                     /locus_tag="VN034_04525"
     CDS             3341..4003
                     /locus_tag="VN034_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HWV59894.1"
                     /translation="MTKPFCLRRRARILSGRLARNVRGAVMIEMAFAIPFLALVGFGG
                     LEIANLTLAHTRVSQLGLNTADNAARIAAGSNLTQPEVREVDINEVFAGAQRQAAGLN
                     FENNGRIILSSLQRNDDGGQTIKWQRCFGNMEDVVSAYGVEGTGATGTDFPGMGPVGR
                     EVTASAGTAIMFVEVTYEYQPLLFGAWLGPQTIRSTAAYNIREARDLSGVKSPGDKAD
                     CD"
     gene            4083..>4808
                     /gene="ubiA"
                     /locus_tag="VN034_04530"
     CDS             4083..>4808
                     /gene="ubiA"
                     /locus_tag="VN034_04530"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HWV59895.1"
                     /translation="MTATHPPDSQLRGFVALLPAAVRPYALLARFDRPIGWWLLYWPC
                     AWAIALGGGAVSHWPLFLWLLLGAIAMRGAGCVYNDIVDRDLDAKVARTASRPVASGA
                     VSVRNALLWTAILSLIGLLVLLQLPGPAQIVALASLILVAGYPFMKRITWWPQAWLGL
                     VFSWGALVAWVAVGGQQGLALPLLYAGSIAWVIGYDTIYALQDIEDDMLVGVKSSARA
                     MGDHVKGGVAMCYAAALGCWGGAL"
BASE COUNT          805 a         1525 c         1612 g          866 t
ORIGIN      
        1 gttaggatcg ccgcgggcgc cggcgccgcg acctcggcaa gcgggacgcc cccggcctcg
       61 tcggcgaaga gcaaagcgcg ctccgtgggc cagtccttca gcagctgcgg caatttgacc
      121 ggcggcgcga gttcggggag cgcggtgcgg ccgcattgtt cgcacgcctc gacgatctgc
      181 gcttcgatcc gctcgctctt gacgcgctcg acgatcgtgc gttcggtgat caccggctgg
      241 agccgcgcga cgcccagttc agtcgccttt tcgatgatcc agtcgagccg cgccttcttc
      301 accggcgcga agcagagcca gaggtcggga acgcgctcca tcgggcgcgt ctgccgctcg
      361 atgcgcagcg tcgccgaacg tttcgcggcg tcggcgacga ccgcgagcca ttcgcccgtc
      421 cggttgtcga agaggaggac gggatcgccc gccttcagcc gcatgacgcc gagcagataa
      481 tgcgcggcgg gcccgtcgag caccggcgcg gcatcaaggc cgagcggttg atcgatgaac
      541 aggcggggcg tgctggcggg cggccaggcg ggagtcgcgg gcatggagcg cgcgatacca
      601 gaaggcggcc tgtcgtgcat cttttgccgc aaggcgctcc tccctgtccc gatccgggtc
      661 agaagcgccg atcgggacag ctccgcgctg cgccgattag cgttttggta agcagttcgc
      721 ccctaggtgc gagagtacca gcggcccatc tggtgggggc gccgcgggcc gaagaggggt
      781 ttgaccatgc gagggacctg gatcagccgg ctgcgcgccg gcgccaaaga gcttctccgc
      841 gaccagcgcg gcaacgcctt catgctgacc gccgcggcga tcgtgccggt gatcggcatc
      901 gtcgggtcgg cggtcgacat cggccgcgcc tatatgacgc agctgcgcct gcagcaggcg
      961 tgcgacgccg gcgtgctcgc cgggcggcgc gcaatggccg gcggcaccta taccgacgat
     1021 gccgaggccg aggcgaacaa gatgttcaac ttcaacttcc ccgaaggcaa atatggcgcg
     1081 tcgggcatcc tcttttcatc cgaagccgac gaggggggct cggacgtcct tggacaggcg
     1141 tcggcggtcc tgcccaccga actgatgttc atgttcggca aggacgaatt ccgcctctcc
     1201 gccaattgca ccgcgaagct cgaaatttcg aacgtcgatg tgatgctcgt gctcgacgtc
     1261 acggggtcga tggaacggac caattcaggc gattcggtcg accgcatcac cgcgctccaa
     1321 gatgcgacga tggacttttt cgacacgttg acgactgcgg atgtcggtga cgggcggctg
     1381 cgcttcggcg tcgtacccta tagctcgaca gcgaatgtcg gcgcgattct tctcgaagag
     1441 aatccggagt ggatttcgga ttttacgatc ctgccgtcgc gttcccccgt catgcgctat
     1501 gactggagcg gcaccaaccc gccgtcatcg gtcacgagcg ggacgacgac caacagcgca
     1561 tgggaagatt tcctgccgat cagcggcttc accagcagcg cggcctgttc ggccgtggtc
     1621 gcgcccggcg acaccatgcc agcgctcagc acggagcagg acatgaacag gacgggctgg
     1681 gccgtcagaa gtaacggtaa ccgcatcttc gccaccgtcg ctggcacgcg gcatcgttac
     1741 tttaattatc gctaccagta taattcgtcg aacagcacct gctggctgca gcgccgcacc
     1801 gtgactttcg atcacgccgc ttcaccgtcg ccgacttcgg gttcaccgtc cttcaacagc
     1861 cagtacaggt acgaggatcg cgtcttcgac gtgtcgaaca tgaaggcagg cgggacgatc
     1921 accgtcaaca ccggcaattc gggcggcaac aggaccttaa gctggagcgg gtgcgtcatc
     1981 gaacgccgca cgtcgccttt cggagcttca tcgtcggcac ccagttctgc gctcgatatg
     2041 gacatcgacc tcgttccaga ggatgaaaac agccgctggc ggattctgct tccggaactc
     2101 tcctatcatc gcgccaccaa cctgaatacc gtgccttcgg gcacgggcgt gcggacagtg
     2161 aacagttcga atgtttccgg tggaaattgg cagagcttcg aatatcactg gcccaatggc
     2221 tggggcgtgt gtccggctgc tgccatgagg ctgacgacga tgacggccga ggatcgctcg
     2281 gccttcaact cctatgtcca gtcgctgcgg ccggttggtg gtacctatca cgatgccggc
     2341 atgatttggg gcgcacggct gctttcgccg agcggcctgt tcgcggacga gaatgcgacg
     2401 gcgccgaata accggccaat cagccgccat atagtcttca tgaccgacgg tgaaatggcc
     2461 ccgaactggc agaatctgac gttccaaggt tatgaatacc tgatgcagcg catcagcggt
     2521 tcggccacga ccgataactc gctactgacc aaccgccaca acaaccgttt cgtgcaattg
     2581 tgccgggcgg cgcgtcagcg cggtatcaca atctgggtgg tctcctttgg cgacgacagc
     2641 aatagtgcag agaatctcgc caacctcaac agttgcgcct cctcgggcac ggcgttcgaa
     2701 gccgacgacg ccgccgagct caacgagcag ttccaggcga tcgcgcggca gatttccaag
     2761 ctgaggctgt cgcaatgacc ggcgtccgtc ccctgctgcg ccggctgcgg cgaagcgagc
     2821 gcggcaccgc gttcgtcgaa ttcgcgctga ccgcgcccgt ctttctgctg atcctgctcg
     2881 gcatctttga ctattgctgg caaatgtatg cgcagcaggt gctgcagggc gtggtggcca
     2941 aggccggccg cgacgcgacg ctcgaaggct ttgccgccga ccaggcgaaa ctcgacaaag
     3001 cggtcgagac gcaggtgaag aaagtgttca agagcgccga agtggatttt aaccggcgcg
     3061 ccttcgacga ttatagcgaa atcaaaccgt tgcgctgggt cgacacgaac ggcaacggcg
     3121 tccaggatcc ctcgcccgac gattgctggg aggatggcgg caagcagggc aatggcggcg
     3181 ccgacgacgt cgtccaatat acggtgtcga tgcagttcga ccgcgtgttg ccggtgtgga
     3241 agatgctggg gcagccgcag tccatgacgc tgacctctac cacgttgctg cgcaaccagc
     3301 cgtttgccgc cgggggtgaa gtgttggtgg agacatgcgg atgaccaagc ctttctgcct
     3361 tcgccgccgc gcgcggatcc tgtcgggccg cctcgcgcgg aacgtccgcg gcgcggtgat
     3421 gatcgaaatg gccttcgcca ttcccttcct cgcgctcgtc ggcttcggcg ggctcgagat
     3481 cgccaatctg acgctcgcgc atacgcgcgt cagccagctc gggctcaaca ccgccgacaa
     3541 tgccgcgcgg atcgcggcgg gcagcaacct gacccagccc gaggtccgcg aggtcgatat
     3601 caacgaagtg ttcgcgggcg cccaacggca ggcggcgggc ctgaatttcg agaataacgg
     3661 ccgcatcatt ctgtcgagcc tccagcgcaa cgacgacggc ggccagacga tcaagtggca
     3721 gcgctgtttc ggcaatatgg aggacgtcgt ttcggcctat ggcgtcgagg ggaccggcgc
     3781 gaccggcacc gattttccgg gcatggggcc cgtcggccgc gaggtgaccg cgtcggcggg
     3841 caccgcgatc atgttcgtcg aagtcactta tgaatatcag ccgctgctgt tcggcgcttg
     3901 gctggggccg cagaccatcc ggtcgaccgc ggcctataat attcgcgagg cgcgcgacct
     3961 gtcgggggtg aagtcgccgg gggacaaggc cgactgcgac tgacggcgta cgcacccggg
     4021 gtggaggcgg cgtcgatcgg gcatttattc gcgaggctgc gcctgctatc gcccgcgcat
     4081 cgatgacggc gacccatccc cccgacagcc agcttcgcgg cttcgtcgcg ctgctccccg
     4141 ccgcggtgcg gccctatgcg ctgcttgcgc gcttcgatcg gccgatcggg tggtggctgc
     4201 tttactggcc gtgcgcctgg gcaattgcgc tcggcggcgg cgcggtcagc cactggccgc
     4261 ttttcctctg gttgctgctc ggcgcgatcg cgatgcgggg tgcgggatgc gtctataacg
     4321 acatcgtcga ccgcgacctc gacgcgaagg tcgcgcgcac cgcgtcgcgg ccggtggcga
     4381 gcggggcggt gtcggtgcgg aacgcgttgc tgtggaccgc gatcctgtcg ctgatcggcc
     4441 tgctcgtgct gctccaattg cccggaccgg cgcagatcgt cgcgctcgcg agcctgatcc
     4501 tcgtcgccgg ctatcccttc atgaaacgca tcacctggtg gccgcaggcg tggctggggc
     4561 tggtgttcag ctggggggcg ctcgtcgcgt gggtcgcggt cggcgggcag caggggctcg
     4621 cgctccctct cctttatgcc ggctcgatcg cgtgggtgat cgggtacgac accatctatg
     4681 cgctgcagga tatcgaggac gatatgctgg tcggcgtgaa gtcgagcgcg cgcgcgatgg
     4741 gcgatcatgt gaagggcggg gtcgcgatgt gctatgcggc cgcgctcggc tgctggggcg
     4801 gcgcgctg
//
LOCUS       DATMSU010000176         4192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145107,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000176 DATMSU010000000
VERSION     DATMSU010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145107"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VN034_04535"
     CDS             <1..231
                     /locus_tag="VN034_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWV59896.1"
                     /translation="DLRALLNPAPAFLASQPVIGLWPVAALGELRAMLDADDLAVRAF
                     ARRIGARAVESDFVPPNINSVADLDRLADDLS"
     gene            complement(228..2498)
                     /locus_tag="VN034_04540"
     CDS             complement(228..2498)
                     /locus_tag="VN034_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HWV59897.1"
                     /translation="MAEHKPRYKTYDSPAGGWGAAAATAKVLMEQSVVTKGSRALLSM
                     NQPGGFKCPSCAFPDATCTKKLEFCENGAKALAHEATKFRVTREFFAQHSVSELMERS
                     DYWLEMQGRLTEPMRYDAATDHYVPVSWDDAFALIGRHLRALASPDQAEFYTSGRTAN
                     ETAFLYSIFVREYGTNNFPDCSNMCHEPTSRGLPHSIGIGKGTVILDDFEHAQAIFLI
                     GHNAGTNAPRMMTPLVEARKRGIPIVAVNPMPERALIKFTEPQDIVQMATFGSSAISS
                     EFVHIKVGGDLALLKGMMKVLFEREAAGEPVLDHDFIAEHTEGFAALKADIEAQQWPD
                     LVAASGIDEAQIRRCAEIYIRSKATLICYGMGITQHQRGSELVQQIANLLLLKGNFGK
                     PGAGISPIRGHSNVQGDRTVGIDEKPSQAYLDRVRDVFGFEPPRAHGHHTVESVEAML
                     AGTAKVFIGLGGNFVRAVPDTDRSYAAMRKLDLTVGIATKLNRGHLVHGRDALILPVV
                     ARSERIETAAGEQFVTIEDSMSNVTASRGVLDPASEHLLPETEIVCRMAMATLPGSKT
                     DWASYIDDYGLIRDKIAEVYPALYEGFAERIEAPLGFHLDIPPRRRAWATPNGKANFL
                     ILPGLAVNAPVDDPAMLRLATVRSHDQFNTTIYSYNDRYRGVYNDRMILFMNADDIAD
                     RGFAAGAKVALETIGADGIARRVEGLTVIDYPMSRGSVAGYYPELNPLLPLAFYDKTS
                     GCPAAKSIPVRVAAVR"
     gene            complement(2576..3667)
                     /gene="pip"
                     /locus_tag="VN034_04545"
     CDS             complement(2576..3667)
                     /gene="pip"
                     /locus_tag="VN034_04545"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HWV59898.1"
                     /translation="MDFSRLQATSKIGDDWVYPQPPCLNFGWLEVDRDPAHRIYWEEY
                     GNPAGEPVMFLHGGPGGACAPAMARFFDPRRYRVILFDQRGCGKSEPNVASAGPAVAL
                     AKNSTADLIGDIEKLREKLEIAGPMHVFGGSWGSTLAMAYAIAHPEHCASLILRGIFL
                     GAPEDLLYLYQGNAATWADDPYGLTEPGAYIKYPGEWADLLSVLTPEERRDVMKSYKA
                     IFDMVPATAAEKERQLKAALTWSLWEGVISNMIPETADTGKFGEADFALCFAQIEAHY
                     FANALFLPAGHFFDNIATLAGIPIHIVHGRFDEVCPLTQASRLVAALRGAGAEPATYV
                     VTNAGHSAMERENALALTAVMDGLAPIGG"
     gene            3900..>4192
                     /locus_tag="VN034_04550"
     CDS             3900..>4192
                     /locus_tag="VN034_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59899.1"
                     /translation="MRFQPILISALGALALSACSQQEEIAAAPPPPPPPGAVPGSIAA
                     DRDGDGIIDGYYTADGMYHPNYTPPPPPPPWPAKSPCAIAPSTSPSCCSRAEKP"
BASE COUNT          694 a         1390 c         1355 g          753 t
ORIGIN      
        1 gaccttcgcg ccttgctgaa ccctgcgccc gccttcctgg ccagccaacc ggtgatcggg
       61 ctgtggcccg tcgcggcgct cggcgaactc cgggcgatgc tcgatgccga cgaccttgcg
      121 gtccgcgcct ttgcgcggcg gatcggcgcg cgcgcggtcg aaagcgactt cgtgccgccc
      181 aatatcaaca gcgtcgccga tctcgaccgg ctggcggacg acctttccta gcgcaccgcg
      241 gcgacgcgca ccgggatcga cttcgccgcc gggcaaccgc tggtcttgtc ataaaaggcg
      301 agcggcagga gcgggttgag ctcgggataa tagcccgcga ccgatccgcg cgacatcgga
      361 tagtcgatca ccgtcagccc ttcgacgcgc cgtgcaatac cgtccgcgcc gatcgtctcc
      421 aacgcgacct tcgcgcccgc cgcgaagccg cggtcggcga tgtcgtcggc gttcatgaac
      481 aggatcatgc ggtcgttata gacgccgcga tagcggtcgt tgtagctgta gatcgtcgtg
      541 ttgaactggt cgtgcgagcg caccgtcgcg agccgcagca tcgcgggatc gtcgaccggc
      601 gcgttgaccg caagtccggg caggataagg aaattcgcct tgccgttcgg cgtcgcccac
      661 gcgcggcggc gcggcgggat gtcgaggtgg aagccgagcg gcgcctcgat gcgttcggca
      721 aagccctcgt agagcgcggg atagacctcc gcgatcttgt cgcggatcag gccataatcg
      781 tcgatatagc tcgcccaatc ggtcttgctg cccggcagcg tcgccatcgc catgcggcag
      841 acgatctcgg tctcgggcag cagatgctcg ctcgcggggt cgagcacgcc gcgtgaggcg
      901 gtgacgttcg acatcgaatc ctcgatcgtg acgaactgct cgcccgccgc ggtctcgatg
      961 cgttcggagc gcgcgacgac gggcaggatc agcgcgtcgc gcccgtgcac cagatgcccg
     1021 cggttgagct tggtcgcgat cccgacggtg agatcgagct ttcgcatcgc ggcataggag
     1081 cggtcggtgt cgggcaccgc gcggacgaaa ttgccgccga ggccgatgaa gaccttcgcg
     1141 gttcccgcga gcatcgcctc gaccgactcg acggtgtgat ggccgtgcgc gcgcggcggt
     1201 tcgaagccga aaacgtcgcg cacccggtcg agataggctt gggacggttt ttcgtcgatc
     1261 ccgacggtgc gatcaccctg cacgttcgaa tggccgcgga tcggcgagat gcccgcgccg
     1321 ggcttgccga aattgccctt gaggaggagc aggttcgcga tctgctggac gagctctgat
     1381 ccccgctgat gctgggttat ccccatgccg tagcagatca gggtggcttt cgagcggata
     1441 tagatttcgg cgcagcgacg gatctgcgcc tcgtcgatcc ccgacgccgc gacgaggtcg
     1501 ggccattgct gggcttcgat atcggccttc agcgccgcga agccctcggt atgctcggcg
     1561 atgaaatcat ggtcgagcac cggctcgccc gccgcctcgc gctcgaacaa gaccttcatc
     1621 atgcccttca gcagcgcgag gtcgccgccg accttgatat ggacgaattc gctgctgatc
     1681 gcgctcgaac cgaaggtcgc catctggacg atgtcctgcg gttcggtgaa tttgatcagc
     1741 gcgcgttcgg gcatcggatt gaccgcgacg atcgggatcc cgcgtttgcg cgcctcgacg
     1801 agcggcgtca tcatccgcgg cgcgttggtc ccggcgttgt ggccgatcag gaaaatcgct
     1861 tgcgcatgct cgaaatcgtc gaggatcacc gtgcccttgc cgatgccgat cgaatgcggc
     1921 agcccgcggc tcgtcggttc gtggcacatg ttcgaacagt cggggaaatt gttcgtccca
     1981 tattcgcgca caaagatcga atagagaaag gccgtctcgt tcgcggtgcg tcccgaggtg
     2041 tagaattcgg cctggtcggg gctggcgagc gcgcggagat gccgcccgat cagcgcgaaa
     2101 gcgtcgtccc agctgaccgg cacataatgg tcggtcgccg cgtcatagcg catcggttcg
     2161 gtcagtcggc cctgcatttc gagccagtaa tccgaccgtt ccatcaactc ggacacgctg
     2221 tgctgcgcga agaattcgcg cgtgacgcgg aatttcgtcg cctcgtgcgc gagcgccttc
     2281 gcgccatttt cgcagaattc gagcttcttg gtacaggtcg catcgggaaa ggcgcagctc
     2341 gggcatttga agccgccggg ctggttcatc gacagcagcg cgcgcgaccc tttggtcacc
     2401 acgctctgct ccatcagcac cttcgcggtc gccgcggccg cgccccagcc gccggcgggg
     2461 ctgtcatatg tcttgtaccg tggtttgtgc tcagccattg cctcccgaat agagcatatc
     2521 ggcaggggct aatccacgag tctttggcgc tggcgacgcc aggcgccgcc ttctatcatc
     2581 cgccgatcgg cgccagcccg tccatcaccg cggtcagcgc gagcgcgttc tcgcgctcca
     2641 tcgcgctatg ccccgcgttg gtcaccacat aggtcgcggg ttccgccccc gcgccgcgca
     2701 gcgccgcgac gagccgcgac gcctgcgtca gcgggcacac ctcgtcgaag cggccatgga
     2761 cgatatggat ggggatgccg gcgagcgtgg cgatattgtc gaagaaatgc cccgcgggga
     2821 ggaacagtgc attggcgaaa taatgcgcct cgatctgcgc gaagcagagc gcgaaatcgg
     2881 cctcgccgaa cttgcccgtg tccgcggtct cggggatcat gttcgagatg acgccctccc
     2941 acagcgacca ggtcagcgcc gccttgagct ggcgttcctt ctcggccgcg gtcgcgggaa
     3001 ccatgtcgaa gatcgccttg taggatttca tcacgtcgcg gcgctcttcg ggggtcagca
     3061 ccgacaagag gtcggcccat tcgcccggat atttgatata ggcgccgggc tcggtgaggc
     3121 cgtagggatc gtccgcccat gtcgcggcat tgccctgata gagatagagc agatcctcgg
     3181 gcgcgccgag gaagatgccg cgcagaatca ggctcgcgca atgttcggga tgcgcgatgg
     3241 cataggccat cgcgagcgtg ctgccccagc tgccgccgaa cacgtgcatc ggccccgcaa
     3301 tctccagctt ctcgcgcaac ttctcgatgt cgccgatcag gtcggcggtg ctgttcttcg
     3361 cgagcgccac cgccgggccc gccgaggcga cattgggttc gctcttgccg cagccgcgct
     3421 ggtcgaacag aatgacgcga tagcgccggg gatcgaaaaa acgcgccatc gcgggggcgc
     3481 aggcgccgcc cgggccgccg tggaggaaca tcaccggctc gcccgcggga ttgccatatt
     3541 cttcccaata gatgcggtgt gcgggatcgc gatcgacctc gagccagccg aaattgaggc
     3601 agggcggctg cggatagacc caatcgtcgc cgatcttgct cgtcgcctgc aatcgcgaaa
     3661 aatccatgtc tttcccttca cttatgaccg gaccgcacag ccggcttggc gaggcatgtc
     3721 cgcgtgtcca ccgcccctgc cgggacgccg ggaacttatg gcgcagatca tcgttcaaaa
     3781 accacccctg atcgcgggat ggtcggcgca tatttggtcg tccgcatgac tcaaatcccg
     3841 gggttgggcg ctcgcgcccg gaaagcgatt tccggtggca atcgaaaagg agtggtctca
     3901 tgcgtttcca acccatcttg ataagcgcgc tcggcgcgct cgcactctcc gcctgttcgc
     3961 agcaggaaga aatcgccgcc gccccgccgc ctcccccgcc cccgggcgcg gttccgggca
     4021 gtatcgccgc cgaccgcgac ggcgacggca tcatcgacgg ctattacacc gccgacggta
     4081 tgtatcatcc gaactacacc ccgccgccgc ccccgccgcc atggcccgcg aaatcgccgt
     4141 gcgcaatcgc cccttcaaca agcccgtcgt gctgctctcg ggcggagaaa cc
//
LOCUS       DATMSU010000177         2156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_21872, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000177 DATMSU010000000
VERSION     DATMSU010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21872"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VN034_04555"
     CDS             complement(<1..486)
                     /locus_tag="VN034_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HWV59900.1"
                     /translation="MITLGFVYVLAGLTFAAFALLGLADRSNPKRFGNAAFWGLLALS
                     MLAGDRLGDFGNGLLVLALVAIAGTGQIGRAPSGDVPAEIQAERAARHGPFLLLVALI
                     IPAVALAGTFLFKWLPGLADPKQATLISLAIGVLIALAVGMARLKPPPLLPLQQGRRL
                     LD"
     gene            complement(483..1154)
                     /locus_tag="VN034_04560"
     CDS             complement(483..1154)
                     /locus_tag="VN034_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HWV59901.1"
                     /translation="MLVLIGILIIIAGFLLRFNPLLVIMASALATGLAAGLDVTAIIA
                     AFGKAFNDTRYVSIVWIVLPVIGLLEAYGLQQHARSLIGRMKGATLGRLLTSYLLLRQ
                     AMAAVGLTSVAGHPQTVRPLVAPMAEAAAEAKNDALTEDQREEVKAFAAATDNVGLFF
                     GEDIFLAIGSILLMKGVLEGYGYIIEPLHFSLWAIPTAIAAFIIHGLRLRRLEKRMMK
                     KEASA"
     gene            complement(1212..>2156)
                     /locus_tag="VN034_04565"
     CDS             complement(1212..>2156)
                     /locus_tag="VN034_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWV59902.1"
                     /translation="ALADLSATHGAQTVAAYMAHGQAQAEAAVRQLIAGLGDGAFRYA
                     MDNGAEVAVAVKIDRTARHVTIDFTGSSATLPDNFNAPLPVVRAAVLYVLRTMLDDPI
                     PMNEGCLAPVTLIVPDDSMLSPRYPAAVVAGNVETSQVITDALFAAFGAMAPAQGTMN
                     NFTFGNDAYQYYETIAGGSGAGPGFDGTSVIQTHMTNSRMTDPEVMETRFPVIVEEFS
                     IRKGSGGAGQWHGGDGAKRRIRFREPMAANILANRRMIAPKGLAGGADAAPGRNWVER
                     ADGRIEILAATGSADLAAGDAFVIETPGGGGYGKPEER"
BASE COUNT          353 a          648 c          811 g          344 t
ORIGIN      
        1 gtcgagcagg cggcggccct gttgcagcgg gagcagcggt ggcggcttga gacgcgccat
       61 gccgaccgcg agcgcgatca gcacgccgat ggcgagcgag atcaaggtcg cctgcttcgg
      121 atcggcgagg ccggggagcc atttgaagag gaaggtgccc gcgagcgcga ccgcggggat
      181 gatcagcgcg acgaggagga ggaaggggcc gtggcgcgcc gcgcgttcgg cctgaatttc
      241 ggcggggaca tcgccgctgg gggcgcggcc gatctgaccg gtgcccgcga tggcgacgag
      301 ggcgaggacg agcaggccgt tgccgaagtc gccgagccgg tcgcccgcga gcatcgacag
      361 cgcgagcagg ccccagaaag cggcgttgcc gaaacgtttg gggttgctgc ggtctgcgag
      421 gccgaggagg gcgaaggcgg cgaaggtcag ccccgcgagg acgtagacga agccgagcgt
      481 gatcatgcgc tcgcctcctt tttcatcatc cgcttttcga ggcggcggag gcggaggccg
      541 tggatgatga aggcggcgat cgcggtcggg atcgcccaga gcgagaagtg cagcggctcg
      601 atgatatagc catagccttc gagcacgccc ttcatcagca ggatcgaacc gatcgcgagg
      661 aagatatcct cgccgaaaaa gagtccgaca ttgtcggtcg cggcggcgaa ggccttgacc
      721 tcttcgcgct ggtcttcggt gagcgcgtcg ttcttcgcct cggcggcggc ttcggccatc
      781 ggggcgacga gcgggcgaac ggtctgcggg tgccccgcga ccgaagtcag gccaaccgcg
      841 gccatcgcct ggcggagcag tagataggag gtgaggagac ggccgagcgt cgcgcccttc
      901 atgcggccga tcaggctgcg ggcgtgttgt tgcaggccgt aagcttcgag caggccgatg
      961 acggggagga cgatccagac gatcgagaca tagcgcgtgt cgttgaatgc cttgccgaag
     1021 gcggcgatga tcgcggtgac gtcgagcccg gcagcaaggc cggtcgcgag cgcggaggcc
     1081 atgatgacga gcagcggatt gaagcggagc agaaagcccg cgatgatgat caggatgccg
     1141 atcagcacga gcatgattgg tttcccttca tcccgtcatc cccgcgtggg gtagaccgcg
     1201 ctgtcgccaa attatctctc ctctggtttc ccgtagccac caccgccggg cgtttcgatc
     1261 acgaaggcgt cgcccgcggc aaggtcggcg ctgcccgtcg ctgccagtat ctcgatccgg
     1321 ccgtcggcgc gttcgaccca gttgcggccc ggtgcggcgt cggcgcctcc cgcgagaccc
     1381 ttcggcgcga tcatgcggcg gttcgcgagg atgttcgcgg ccatcggctc gcggaagcgg
     1441 atgcggcgct tggcgccgtc gccgccgtgc cattggcccg caccgcccga gcctttgcga
     1501 atcgaaaatt cctcgacgat cactgggaag cgcgtttcca tcacttcggg gtcggtcatc
     1561 cggctgttgg tcatatgcgt ctggatcacg ctcgtgccgt cgaagcccgg gcccgcgccc
     1621 gagccgccgg cgatcgtttc gtaatattga tacgcgtcgt tgccgaaggt gaaattgttc
     1681 atcgtgccct gcgcgggcgc catcgcgccg aaggcggcga acagcgcgtc ggtgatgacc
     1741 tggctcgtct cgacattgcc cgcgacgacc gcggcgggat agcggggtga gagcatcgaa
     1801 tcgtcgggga cgatcagcgt gacgggggcg aggcagccct cgttcatcgg gatcggatcg
     1861 tcgagcatcg tgcgcagcac atagaggacg gcggcgcgga cgacgggcag cggggcgttg
     1921 aaattgtcgg ggagggtggc gctggagccg gtgaaatcga tggtgacgtg gcgggcggtg
     1981 cggtcgattt tcaccgcgac cgcgacctcg gcgccattgt ccatcgcgta gcggaaggcg
     2041 ccgtcgccca agcccgcgat gagctggcgg acggcggctt cggcctgagc ctggccgtgc
     2101 gccatatagg cggcgacggt ttgagcgccg tgcgtcgcgg agaggtcggc gagcgc
//
LOCUS       DATMSU010000178         3396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_173872,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000178 DATMSU010000000
VERSION     DATMSU010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173872"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VN034_04570"
     CDS             complement(<1..618)
                     /locus_tag="VN034_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWV59903.1"
                     /translation="MSARKTAIVVAPGRGTYGKGELGSIARLHGARFGELIANFDAQR
                     RDRGQPTVTELDGADRFSVGVHMRGDVAAPLIYTATALDYLSIDRERYDIVAALGNSM
                     GWYSALALGGAVSIEDGFRISNAMGLNSQTHGPGGQILLQVVDEDWRPVPGLREKLLD
                     LVAAIDTRPDHALALSIDLAGMLVFAGNEEGLAALLAEAPPTPGRD"
     gene            complement(615..2816)
                     /locus_tag="VN034_04575"
     CDS             complement(615..2816)
                     /locus_tag="VN034_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWV59904.1"
                     /translation="MDAAQAVHEKFLGALAGGRLPDRADAPDPAVAGLSRADATDIFL
                     SQLTSRQMDRLSRHLQARGEGFYTIGSSGHEGNAAVAAALRVTDMAFLHYRSNAFQLH
                     RARQLPGGTPTWDMLLSFAASSEDPISGGRHKVIGSKPLAIPPQTSTIASHLPKAVGA
                     AFSIGIARRLGMTGLPLPDDAVVLTSFGDASANHSTAQGAFNTAGWAAFQGSPMPLIF
                     LCEDNGIGISTRTPTGWIEAQFRHRAGLHYIKCDGTDLVSAYAGAAEAADYARRTRKP
                     VFLHMGTVRLYGHAGSDVQGAYLPKALIEADEARDPLLAGAALMAEHGWMSAAEIAEA
                     YEEIGATLARQAEAAIRRPKIITPAHVMQSLIPPGREVARTNTPSDEDRKAMFGSDAG
                     QMDKPMHMARLLSWALADLMLAHKEIVVAGEDVGPKGGVYNVTAKLHQRFGSARVVNT
                     LLDEQAILGLAIGMAHNGFVPMPEIQFLAYVHNAEDQIRGEAATLSFFSDGQYSNPMV
                     IRIAGLGYQKGFGGHFHNDNSLAVFRDIPGVILAVPSNGRDAVQMLRECVRLAREEQR
                     VIVFVEPIALYMTRDLHEEGDGLWTSVYEAPGAGAPIRLGDVGVHGDGTDLAIVTYGN
                     GYYLSRQAEKLLADDGVKARVIDLRWLGPVDEAKLVAAVGDAMRILIVDECRITGSQS
                     EALMATFVERTPNKKLARIAADDSFIPLGKAATLTLPSRDSIVAAAKELLA"
     gene            2992..>3396
                     /locus_tag="VN034_04580"
     CDS             2992..>3396
                     /locus_tag="VN034_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWV59905.1"
                     /translation="MWIAQTSAAADTSGAGGGQGWLLDPELVDPAYSQTIAGGEIQTA
                     FPPPPPPPPPTPEWLKSLLNAIGNFFEWSAPAAKPLMWIVIALILLFLLYHFVPAFAR
                     WVDNLRFGRKATGDEEDDIGRAEAGAARALLAE"
BASE COUNT          522 a         1153 c         1151 g          570 t
ORIGIN      
        1 atcgcgcccc ggggtcggcg gcgcttcggc gagcagcgcg gcgagcccct cctcattgcc
       61 cgcgaacacg agcatgccgg cgaggtcgat cgacagcgcg agcgcgtggt ccggacgggt
      121 gtcgatcgcc gcgacaaggt ccagcagctt ttcgcgcagc cccggtaccg gacgccaatc
      181 ctcgtcgacc acttgcaaca atatctgccc gccgggaccg tgcgtctggc tgttgaggcc
      241 catcgcgttc gatatgcgga agccgtcctc gatcgacacc gcgccgccga gcgccagcgc
      301 gctgtaccag cccatcgagt tgccgagcgc cgcgacgatg tcgtatcgtt cgcggtcgat
      361 gctcagataa tcgagcgcgg tcgcggtgta gatcagcggc gccgcaacgt cgccgcgcat
      421 atgcaccccg acgctgaagc ggtcggcgcc gtcgagctcg gtgaccgttg gctggccgcg
      481 gtcgcggcgc tgggcatcga agttcgcgat cagctcgccg aagcgcgcgc cgtgcaaccg
      541 cgcaatactc ccgagctcgc ccttgccata ggtgccgcgg ccgggggcga cgacgatcgc
      601 ggtctttcgc gcgctcatgc cagcagctcc ttcgcggcgg cgacgatgct gtcgcggctc
      661 ggcagcgtca gcgttgccgc cttgcccaga gggatgaagc tgtcgtcggc ggcgatgcgc
      721 gccagcttct tgttcggagt ccgctcgacg aaggtcgcca tcagcgcctc gctctgcgat
      781 ccggtgatgc ggcattcgtc gacgatcagg atgcgcatcg catcgccgac ggccgccacc
      841 agcttcgctt cgtcgaccgg gccgagccag cggagatcga tcacgcgcgc cttgacgcca
      901 tcgtcggcga gcagtttttc ggcctgccgc gacagataat atccattgcc gtaagtgacg
      961 atcgcgaggt cggtgccgtc gccgtggacg ccgacgtcgc cgaggcgaat cggcgccccc
     1021 gcgcccggcg cctcatatac gctggtccac aggccgtcgc cttcttcgtg gaggtcgcgc
     1081 gtcatataga gcgcgatagg ttcgacgaag acgatcacgc gctgctcctc gcgcgccagc
     1141 cgcacgcatt cgcgcagcat ctgcaccgcg tcgcgtccgt tcgagggcac cgcaagaatg
     1201 acgcccggaa tgtcgcggaa gaccgcgagg ctgttgtcgt tatggaaatg gccgccgaag
     1261 cccttctgat agccgagccc cgcgatgcgg atgaccatcg gattgctata ttgcccgtcg
     1321 gagaagaagc tgagcgtcgc cgcctcgccg cggatctggt cctcggcatt gtggacatag
     1381 gcgaggaact ggatctcggg catcggaacg aaaccattgt gcgccatgcc gatcgcgagc
     1441 ccgaggatcg cctgttcgtc gagcaaagta ttgaccacgc gcgccgaccc gaagcgctgg
     1501 tggagcttcg ccgtgacgtt gtagacgccg cccttcggcc cgacatcttc gcccgcgacg
     1561 acgatctcct tgtgcgcgag catcaggtcg gcgagcgccc aggagaggag ccgcgccatg
     1621 tgcatcggct tgtccatctg gccggcgtcg ctgccgaaca tcgccttgcg atcctcatcc
     1681 gacggcgtgt tcgtccgcgc cacctcgcgc cccggcggga tcaggctttg catcacatgc
     1741 gccggcgtta tgatcttggg gcggcggatc gccgcttcgg cctgccgcgc aagcgtggca
     1801 ccgatctcct catatgcttc ggcgatctcg gccgcgctca tccagccatg ttcggccatc
     1861 agcgccgcgc ccgcgaggag cgggtcgcgc gcctcgtcgg cctcgatcag cgccttggga
     1921 agataggccc cctgcacatc cgatccggcg tggccataga gacggacggt gcccatgtgg
     1981 aggaacacgg gcttgcgggt acgccgcgca taatcggccg cctcggcggc gcccgcgtag
     2041 gccgagacga ggtcggtgcc gtcgcatttg atgtagtgga gaccggcgcg gtggcggaac
     2101 tgcgcctcga tccagccggt cggggtgcgc gtcgaaatgc cgatgccatt gtcttcgcaa
     2161 aggaagatca gcggcatcgg cgagccctgg aaggccgccc agcccgcggt gttgaacgcg
     2221 ccctgcgcgg tcgaatgatt cgccgaggcg tcgccgaagc tcgtcagcac caccgcgtcg
     2281 tcgggcagcg gcagcccggt catgccgagg cggcgcgcga tgccgatcga aaaggccgcg
     2341 ccgaccgcct tgggcaaatg cgaggcgatc gtcgaggttt gcggcgggat ggcgagcggc
     2401 ttcgacccga tcaccttgtg ccgcccgcct gaaatcggat cctccgacga tgcggcgaag
     2461 ctcagcagca tgtcccatgt cggcgtgccg ccgggaagct ggcgcgcgcg gtggagctgg
     2521 aaggcgttcg agcgatagtg aaggaaggcc atgtcggtga cgcgcagcgc cgccgcgacc
     2581 gccgcattgc cctcgtgccc cgacgagccg atcgtgtaga aaccttcgcc gcgtgcctgc
     2641 agatggcgcg acagccggtc catctggcgg ctcgtcagct gcgagaggaa gatgtcggtg
     2701 gcgtcggcgc gcgacagccc ggccacggcg ggatcgggcg cgtcggcgcg atcgggcagc
     2761 ctgccgcccg ccagcgcgcc aaggaatttc tcatgtaccg cctgcgccgc gtccacctgt
     2821 ctctcctgtc gcgcggccgg tggtttccac ggtaacgaac cggccgattt ttccgcgcgc
     2881 tagaacgggg cagttcggcg cgcaagcatc tcttgccttg cggagcacgc tcgcgctacc
     2941 aagatcggca atgaccgagc ggggcgagat catcttttca acatgatatc catgtggatc
     3001 gcccagacgt cggctgcggc cgacacgtcc ggcgccggtg ggggtcaggg gtggctgctc
     3061 gaccccgaac tcgtcgaccc ggcttatagc cagacgatcg cgggcggcga gatccagacc
     3121 gcctttccgc cgccgcctcc acccccgccg ccgacgcccg agtggttgaa gtcgctgctc
     3181 aacgcgatcg gcaatttctt cgagtggagc gcgcccgcgg cgaagccgct gatgtggatc
     3241 gtgatcgcgc tgatcctgct gttcctcctt taccatttcg tccccgcctt tgcgcggtgg
     3301 gtcgataatc tgcgcttcgg ccgcaaggcg acgggcgatg aggaggatga tattggccgg
     3361 gccgaggccg gcgcggcgcg cgcgctgctc gccgag
//
LOCUS       DATMSU010000179         5406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_21882, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000179 DATMSU010000000
VERSION     DATMSU010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21882"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VN034_04585"
     CDS             complement(<1..800)
                     /locus_tag="VN034_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWV59906.1"
                     /translation="MSDIDSVERLEAILGKTPPAVNLKVIDHVDASAARWLAASPLMF
                     AGFGDGEAGIAVTLGGGAPGFAGSEPGTLSVPLAALDDPALAVPGASFGSLFLVPGIG
                     ETLRINGHVAVVADGIARIAVAECYVHCAKALIRSGFWSADPSDAAPSTPSEFAAATR
                     FMALATIDAAGAADLSPKGDPAGTMVHAAGDQLWFADRPGNRRTDSFRNLIAQPRIAA
                     ALLIPGSADVAILGGTAMLTEDETARATFAVQGKVPLLATRVDDVVIER"
     gene            complement(815..2980)
                     /locus_tag="VN034_04590"
     CDS             complement(815..2980)
                     /locus_tag="VN034_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HWV59907.1"
                     /translation="MTRSTFRTALGLSAATAVLAVAAPAMAQEASAGEDIVVTAQRSN
                     ETQVIRGGSLGALGAKDAMDTPFAVKSYNEALILNQQPLTLGAVLENDPSVRTTLGFG
                     NMSEQFVVRGFNLFGEDIAIDGLFGVTPRQLVSPELYDQVQILNGASAFLFGAAPGGS
                     ALGGTVNLTPKRAKDQDTSRLTANYLSDAHFGGAFDLGRRFGTDGAFGARINGAFRSG
                     DIAIDDEFRRSAVIGGSFDWRTDNLRLSLDLAYQNVKAKHMRPMVQLNNKYGTVTAIP
                     RPPRADLNYGQPWYNQSIRDIFGIFKLEYDVTENLMLYASAGARDTAERGIYQGFQLL
                     DSTTGDAFVTGSNIPRNDNNEAAQAGLRFSGESLGITHQLNMGYSHSRYINRNAFEFG
                     RFDDDALGGTNLNNLYAPRTVALPTFDAFVAGDLADPLPANRTRLDSIFVSDTLGFAG
                     DMVQLTLGLRHQNIHFRTYNITTGAQNTEYNRGTTTPVVGVVIRPREKISLYANRIEA
                     LIQGPVAGAGTINVGQVFPPFQAVQYEIGGKLALGRFNASLAAFQTDRPQTLNEVTPD
                     GTVFTLNGQQRNKGIELSVDGEPVDGLRIIAGVSVTDAKQRRTAGGATDGLDAIGVPE
                     YTANANIEWDVYGGFTLTGRVIQTGKQAVNLTNTLELPEWTRFDLGARYVVAAGDTPI
                     TFRFNVDNIANKRYWSSSLGGYLVQGLPRTFKTSVTAEF"
     gene            complement(3027..3206)
                     /locus_tag="VN034_04595"
     CDS             complement(3027..3206)
                     /locus_tag="VN034_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59908.1"
                     /translation="MKKANGLRAIFAVPLLLALASLVGLVVALTGDGWRDAISWALLA
                     IPVLAVGWAMRARRT"
     gene            complement(3203..4369)
                     /locus_tag="VN034_04600"
     CDS             complement(3203..4369)
                     /locus_tag="VN034_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWV59909.1"
                     /translation="MQRTTIRTWFLIHKWTSIVCTAFLLMLCVTGLPLIFHEEIDLLT
                     EETPQYGLPGVGSSGEAPGLKPLDEMLAKALAARPGEVPVFMAFDNEQPSMTVTTAPR
                     ADSPAADMTIQILDRSTGEATSTVDESGAMHFLLQLHTDMFLGLPGMLFLGLMGLFFV
                     IAVVSGVVLYAPFMKKLDFGTLRTSRSRRVKWLDYHNLLGIVALAWMTVVGATGVINA
                     LATPIFEYWQRTDLAEMTRAYDGKGAVPPELYGSIDKAMAAARKEIPGNNPQFIAFPG
                     GAFSSKHHYAVFFQGDTPLTEGLLTAALIDAETGEFTDARAMPWYAKALALSQPLHFG
                     DYGALPLKILWAILTLFTMIVLGSGLYLWLGKRRSGTDALVREVESGGTLVPAE"
     gene            4614..5090
                     /locus_tag="VN034_04605"
     CDS             4614..5090
                     /locus_tag="VN034_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWV59910.1"
                     /translation="MPLSREMQRLRAEHAALVTLSRIILDMVHAPHPPPAAELAAARG
                     KLRENLVRHLKCEDWILYPRLMATGDQELMRITRDFEIEMGGLAADFGAYDEKWTGER
                     VAADWPGFGRETVAIFDILAARVEREERELYPLAESLYASGAAIGVPVMDGSTISS"
     gene            complement(5062..>5406)
                     /locus_tag="VN034_04610"
     CDS             complement(5062..>5406)
                     /locus_tag="VN034_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59911.1"
                     /translation="VGGATFGRAFDDGLVNGDRGYAAFGELALRPLRSGSFSKSEIYG
                     FVDYADVTLLERPALPRTDFTLGSWGGGVRLSYAENATIGLEIADAWDQPVPGYGQDW
                     RVALSWKLSIRP"
BASE COUNT          933 a         1671 c         1874 g          928 t
ORIGIN      
        1 cgttcgatca cgacatcatc gacgcgcgtt gcgagcagcg gcaccttgcc ctgcaccgcg
       61 aaggtcgcgc gcgcggtttc gtcctcggtc agcatcgcgg tgccgccaag gatcgcgacg
      121 tccgccgaac cggggatcag cagcgcggcg gcgatgcgcg gctgcgcgat gagattacgg
      181 aagctgtcgg tgcgccggtt gccggggcgg tcggcgaacc agagctgatc gcccgcggcg
      241 tgcaccatcg tcccggcggg gtcgcccttg gggctgaggt cggccgcgcc tgcggcgtcg
      301 attgtcgcga gcgccatgaa gcgcgtcgcc gcggcgaatt cgcttggggt agatggagcg
      361 gcgtccgacg gatcggccga ccagaagccc gagcggatca gcgccttggc gcagtggaca
      421 tagcattcgg cgacggcgat gcgggcgatg ccatcggcga ccacggcgac atggccgttg
      481 atgcgcagcg tctcgccgat gccggggacg aggaagagcg agccgaagct ggcgccgggc
      541 accgcgagcg cgggatcgtc gagcgcggcg agcgggaccg agagcgtccc cggttcgctg
      601 ccggcaaaac cgggcgcgcc gccgccgagc gtgaccgcga tgcccgcttc accatcgccg
      661 aatcccgcga acatcagcgg cgaagcggcg agccagcgcg ccgcgctcgc atcgacatgg
      721 tcgatcacct tgagattcac cgcgggcggc gtcttcccga ggatcgcttc gaggcgttcg
      781 acgctgtcga tgtcgctcac atgtgcctcc cgtatcagaa ttccgcggtg accgacgtct
      841 tgaaggtgcg cggcagaccc tgaacgagat agccgccgag cgacgacgac cagtatcgct
      901 tgttggcgat attatcgacg ttgaatcgga aggtgatcgg cgtgtcgcca gccgcgacga
      961 catagcgcgc gccgagatcg aaacgggtcc attcgggcag ctcgagcgta ttggtgagat
     1021 tcaccgcctg cttgccggtc tggatgacac ggccggtcag cgtaaagccg ccatagacat
     1081 cccactcgat attggcgttg gcggtgtatt cgggcacgcc gatcgcatcg agcccgtcgg
     1141 tagctccgcc agcggtacgg cgctgcttgg catcggtgac cgagacgccc gcgatgatgc
     1201 gcaggccgtc gaccggctcg ccatcgacgc tgagttcgat gcccttgttg cgctgttgcc
     1261 cgttgagggt gaagaccgtg ccgtccggtg tcacttcgtt cagcgtctgc gggcgatcgg
     1321 tctggaatgc cgcgagcgag gcgttgaagc ggccgagcgc gagtttgccg ccgatctcat
     1381 actgcaccgc ctgaaagggc ggaaacacct gcccgacatt gatcgtgccg gccccggcga
     1441 ccggaccctg gatcagcgct tcgatgcgat tggcgtagag cgagattttc tccctggggc
     1501 gaatgacgac gccgaccacg ggggtggtcg tgccgcggtt atattcggtg ttctgcgcgc
     1561 ccgtggtgat attgtaggtg cgaaaatgga tattctgatg ccgcaggccg agcgtcagct
     1621 gaaccatatc cccggcaaag ccgagcgtgt cggacacgaa gatgctgtcg aggcgcgtgc
     1681 ggttcgcggg gagaggatcg gcgaggtcgc ctgcgacaaa cgcgtcgaac gtcggcagcg
     1741 cgacggtgcg cggcgcatag aggttattga ggttggtgcc gcccagcgca tcgtcatcga
     1801 accggccaaa ttcaaaggcg ttccggttga tgtagcggct gtgcgaatag cccatgttga
     1861 gctgatgggt gatgcccaag ctttcaccgc tgaaccgcag ccccgcctgt gcggcttcgt
     1921 tattgtcgtt gcgcgggatg ttcgagccgg tcacgaacgc atcgccggtg gtcgaatcga
     1981 gaagctggaa gccctgatag atcccgcgtt cggccgtgtc gcgtgcgccc gccgacgcgt
     2041 agagcatcag attctcggtc acgtcatatt cgagcttgaa gatgccgaag atgtcgcgga
     2101 tcgactgatt ataccatggc tggccatagt tgaggtccgc gcgcggcggg cgcggtatcg
     2161 cggtgaccgt gccatatttg ttgttgagct gaaccatcgg gcgcatgtgc ttcgccttga
     2221 cgttctgata ggcgaggtcg aggctgaggc gcagattgtc ggtgcgccag tcgaagctgc
     2281 cgccgatcac cgccgaacgg cggaactcgt cgtcgatcgc gatgtcgccc gatcggaatg
     2341 cgccgttgat acgcgcgccg aacgcaccat cggttccgaa acggcggcct aaatcgaacg
     2401 ccccgccgaa atgcgcgtcg gagagatagt tggccgtaag gcgactggtg tcctgatcct
     2461 tcgcgcgctt gggcgtcagg ttgacggtgc cgcctagcgc ggaaccgccg ggcgcggcgc
     2521 cgaacagaaa ggcgctggcg ccgttcagga tctggacctg atcataaagt tcgggagaga
     2581 cgagctggcg cggcgtcacc ccgaacaggc cgtcgatcgc aatgtcttcg ccgaagaggt
     2641 tgaagccgcg gaccacgaac tgctcggaca tgttaccgaa gccgagcgtc gtgcgaaccg
     2701 acgggtcatt ctcgagcacc gcgccgagcg tcagcggctg ttggttgagg atcaacgctt
     2761 cgttgtagct cttgactgcg aacggcgtat ccatcgcgtc cttggcgcca agggcgccga
     2821 gcgagccgcc gcgtatgacc tgcgtctcgt tgctccgctg cgcggtgacg acgatgtctt
     2881 cgccggcgct cgcctcctgc gccatggcgg gcgcggcgac ggcgagcacc gcggtcgcgg
     2941 cggagaggcc gagcgcggtg cggaaggtcg atctggtcac tgatattccc cttaaaagcg
     3001 cggccctacc ccgcgcgtcc tgtgtttcag gttcggcgtg cgcgcatcgc ccagccgacg
     3061 gccaataccg gtatggcgag cagcgcccac gagatggcgt cgcgccagcc gtcgccggtc
     3121 agcgcgacga cgaggccgac gaggctcgcg agtgcgagca gcagcgggac cgcgaagatc
     3181 gcgcggaggc cgttcgcctt cttcattcgg ccggcaccaa agtgccgccg ctctcgacct
     3241 cgcgcaccag cgcgtcggtg ccggagcggc gcttgccgag ccagagatag aggcccgagc
     3301 cgaggacgat catcgtgaag agggtgagga tcgcccaaag gatcttgagc ggcagcgcgc
     3361 cataatcgcc gaagtgcagc ggctgcgaca gcgcgagcgc cttcgcatac cagggcatcg
     3421 cgcgcgcgtc ggtgaactcg cccgtttcgg cgtcgatcag cgcggcggtg agcagccctt
     3481 cggtcagcgg cgtgtcgccc tggaagaaga cggcataatg atgcttgctc gaaaaggcgc
     3541 cgccggggaa ggcgatgaac tgcggattgt tgccggggat ctccttgcgc gccgccgcca
     3601 tcgccttgtc gatcgagccg taaagttcgg gcggcaccgc gcccttgccg tcataggcgc
     3661 gcgtcatttc ggcgaggtcg gtccgttgcc aatattcgaa gatcggggtc gcgagcgcgt
     3721 tgatcacgcc ggtcgcgccg accaccgtca tccacgcgag cgcgacgatg ccgagcaaat
     3781 tgtgatagtc gagccatttg acgcggcggc tgcgcgaggt gcgcagggtg ccgaagtcga
     3841 gctttttcat gaagggcgcg tagagcacga ctcccgagac gaccgcgatc acgaagaaca
     3901 gccccatcag cccgagaaag agcataccgg ggaggccgag gaacatgtcg gtgtggagct
     3961 ggagcaggaa atgcatcgcg ccgctttcgt cgacggtcga ggtcgcttcg ccggtggagc
     4021 ggtcgaggat ctggatcgtc atgtcggcgg cgggcgaatc ggcgcgcggc gcggtggtga
     4081 ccgtcatcga cggctgctca ttgtcgaacg ccatgaacac cgggacttcg cccggccgcg
     4141 cggcgagcgc cttcgccagc atttcgtcga ggggtttgag gccgggggcc tcgcccgacg
     4201 atccgacccc gggcaggcca tattgcgggg tttcctcggt cagcaggtcg atttcctcgt
     4261 gaaagatcaa cggcaggccg gtgacgcaca gcatcagcag aaaggcggtg cagacgatgc
     4321 tcgtccattt atggatcagg aaccatgtcc ggatggtcgt gcgttgcatg gggaaatact
     4381 gctgctattg cgaggaaaaa tcaatagcgc cctaggattt gccgtcccgg ttggcaaccc
     4441 gattcgcgcc gcgcacccca tcgttcgggc cagctatgcc atcgatcggg ccagcccggc
     4501 ggcacgcggt ccggtgcgcg tcgcgtgcgc tatttccttg ccgattcact ttcctttcat
     4561 cgccgattgc ctatgatgga ggccgaggga ccgctcgcat cgatggagca atgatgccct
     4621 tgagccgcga gatgcagcga ctgcgcgccg aacatgcggc gctcgtcacc ttgtcgcgga
     4681 tcatcctcga catggtccat gccccgcacc cgccgcccgc ggccgagctg gcggcggcgc
     4741 gcgggaagct ccgcgaaaat ctggtgcgcc atctgaaatg cgaggactgg atattatatc
     4801 cgcggctgat ggcgacgggc gatcaggaac tgatgcggat cacgcgcgac ttcgaaatcg
     4861 agatgggcgg tcttgccgcc gatttcggcg cctatgacga gaaatggacg ggtgagcgcg
     4921 tcgcggccga ctggccgggt ttcggccgcg agacggtcgc gatcttcgac atcctcgcgg
     4981 cgcgcgtcga gcgcgaggag cgcgaactct atccgctcgc cgaaagcctt tatgcgtcgg
     5041 gcgccgcgat cggcgtgccg gtcatggacg gatcgacaat ttccagctga gcgcgacgcg
     5101 ccagtcctga ccatagccgg ggaccggctg gtcccacgca tcggcgattt cgaggccgat
     5161 ggtggcgttt tcggcatagg agaggcgcac cccgccgccc cagctcccga gagtgaaatc
     5221 ggtgcgcgga agcgcggggc gttcgagcaa tgtcacgtcg gcataatcga cgaagccata
     5281 gatctcgctc ttcgaaaaac tgccgctgcg caagggacgg agcgcgagtt cgccgaacgc
     5341 cgcatagccg cgatcgccgt tgaccagccc gtcgtcgaac gcgcggccga aggtcgcgcc
     5401 gccgac
//
LOCUS       DATMSU010000180         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_135723,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000180 DATMSU010000000
VERSION     DATMSU010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135723"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VN034_04615"
     CDS             <1..1245
                     /locus_tag="VN034_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWV59912.1"
                     /translation="ERLRPRTLADVVGQEHLTGPEGAIGRMVAAGQLSSIILWGPPGT
                     GKTTIARLLAEAVGMRFAPLSAVFSGVADLRQAFADAEKMAAAGKRTLLFVDEIHRFN
                     RAQQDGFLPYVERGTVVLVGATTENPSFALNAALLSRAQVLVLNRLGEGALGTLIERA
                     EAEIGQHLPVTDAARAALIASADGDGRFLLNQVETLFSVTIENPLDPDELAQFLHRRM
                     PVYDKGGDGHYNLISALHKALRGSDPQAALYWLARMLVAGEEPLYVLRRLTRFASEDI
                     GLADPQALVQCLAAKDAYQFLGSPEGELAIVQACLYLATAPKSNAAYAAQKAAWASAR
                     ETGSLAPPANILNAPTKLMKDLGYGAGYSYDHDAPDGFSGDNYWPDEMAPADFYRPVD
                     RGFEKRIAERLAWWDERRRAKE"
     gene            1366..>1984
                     /locus_tag="VN034_04620"
     CDS             1366..>1984
                     /locus_tag="VN034_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59913.1"
                     /translation="MALALVLAAAATPVLSCPGGTIEIPCTAAEVAAKIAVTRARLAN
                     IAQRCLYDFGGKCTVEASGRINTADRGTTLLWQKMLLAPRDGAQTRMLVLVAQDKAGK
                     PTLAGFAESSGSIGTPDLVVDNDRRRLIHVGGTLAGSGGGNADALFMNDVAGPGWRRI
                     DLSDWAEQGGKMLPTGYWLRGPAEFAFAEMAASAPVARDGDGNCCP"
BASE COUNT          312 a          673 c          674 g          325 t
ORIGIN      
        1 gagcggctgc gcccgcgcac cctcgccgat gtcgtcgggc aggagcatct gaccggtccc
       61 gaaggcgcca tcggccgcat ggtcgccgcg gggcaattgt cgtcgatcat cctctggggt
      121 ccgccgggga cgggcaagac aacgatcgcg cggctgctcg ccgaggcggt cggcatgcgc
      181 ttcgccccgc tgtcggcggt cttctcgggt gttgctgacc tgcggcaggc cttcgccgat
      241 gccgagaaga tggcggcggc gggcaagcgt accctgcttt tcgtcgacga gatccatcgc
      301 ttcaatcgcg cccagcagga cggcttcctg ccctatgtcg agcgcggcac cgtggtgctc
      361 gtcggcgcga cgaccgagaa tcccagcttt gcattgaacg ccgcgctgct cagccgtgca
      421 caggtgctcg tcctcaatcg gctgggcgag ggcgcgctcg gcacgctgat cgagcgtgcc
      481 gaagccgaga tcggccagca cctgccggtg accgacgcgg cgcgcgcggc gctgatcgcc
      541 agcgccgacg gcgacgggcg cttcctgctc aatcaggtcg aaaccctgtt ttcggtgacg
      601 atcgaaaatc cgctcgatcc agacgaactc gcgcaattcc tccatcgccg gatgccggtc
      661 tatgacaagg gcggcgacgg ccactacaat ctgatctcgg cgctgcacaa ggcgctgcgc
      721 ggatcggacc cgcaggcggc gctctactgg ctcgcgcgga tgctcgtcgc gggcgaggag
      781 ccgctctatg tgctgcggcg actcacccgc ttcgcgagcg aggatatcgg cctcgccgat
      841 ccgcaggcgc tggtgcaatg cctcgccgcg aaggacgcct atcagttcct cggttcgccc
      901 gagggcgagc tcgcgatcgt ccaggcctgc ctctatctcg ccaccgcgcc caaatcgaac
      961 gccgcctatg ccgcgcagaa ggccgcgtgg gcgtcggcgc gcgaaaccgg cagcctcgcg
     1021 ccccccgcga acatcctcaa tgccccgacc aagctgatga aagatctggg ttatggcgcg
     1081 ggctatagct acgaccatga cgcccccgat ggcttttcgg gcgacaatta ctggcccgac
     1141 gaaatggcgc ccgcggactt ctatcgcccc gtcgatcgcg gcttcgaaaa gcgcatcgcc
     1201 gagcgcctcg cctggtggga tgagaggcgc cgcgcgaagg agtgacgctg agtcccatgc
     1261 cgtcttcgac gtggcggtat gggtatttga ctagccttgc cccgggcgcc gcaagggtat
     1321 aatttttcgc tccgtcgggt cgcgccggaa aacggggtag tcatcatggc attggcgctt
     1381 gtccttgccg ccgcagcgac accggtcctg tcctgtccag gcggcaccat cgaaatccca
     1441 tgcacggcgg cggaggtcgc ggcgaagatc gcggtgacgc gcgcgcgcct tgccaatatc
     1501 gcgcagcgat gcctctatga cttcggcgga aaatgcaccg tcgaggcgag cgggcggatc
     1561 aacaccgcgg atcgcggcac aaccttgctc tggcaaaaga tgctgctcgc cccgcgcgac
     1621 ggcgcccaaa cgcggatgct cgtgctcgtt gcgcaggaca aggcgggcaa gccgacgctc
     1681 gcgggtttcg ccgaaagctc gggatcgatc ggcacgcccg atctggtcgt cgacaacgac
     1741 cggcgccggc tgatccatgt cggcggtacg cttgccggca gcggcggcgg caatgcggac
     1801 gcgctgttca tgaatgatgt ggctgggccg gggtggcgcc gcatcgacct gtccgactgg
     1861 gcggagcagg ggggcaagat gctgccgacc ggctattggc tgcgcggccc ggccgagttc
     1921 gcgttcgccg aaatggccgc gtcggccccc gtcgcgcgcg acggcgacgg caattgctgc
     1981 ccgc
//
LOCUS       DATMSU010000181         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145137,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000181 DATMSU010000000
VERSION     DATMSU010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145137"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VN034_04625"
     CDS             <1..290
                     /locus_tag="VN034_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HWV59914.1"
                     /translation="ARAIARGIVARGWHEDWPDARRQFACLPFQHSEDLGDQRESLRL
                     FGQFDDPMFQDYAQKHFDIVDRFGRFPHRNEALGRATRADEEAAIEEGKNW"
     gene            319..882
                     /locus_tag="VN034_04630"
     CDS             319..882
                     /locus_tag="VN034_04630"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWV59915.1"
                     /translation="MAEFTDTLTDKHIAFIEKQPVFFTATAAADGRINLSPKGYADSF
                     KILSDAQVAYLDLGGSGNETHAHLAADGRITIMFCAFDRSALILRIYGRGRPVLPQDG
                     EWDALAAHFTLLPGTRQIFVIDVESVQTSCGWGVPMMELQHERDTLQKYHRQADRALW
                     VEKFKERDRSIDGLPTRPTDRFIAGDA"
     gene            882..1112
                     /locus_tag="VN034_04635"
     CDS             882..1112
                     /locus_tag="VN034_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HWV59916.1"
                     /translation="MPLVELVRLPNGAEAELLRGRLESAGVHAVCFDAGMNIAESVGL
                     LIPVRIMVLDEDLGEARALMAEFEGGGNGNAA"
     gene            1128..1832
                     /locus_tag="VN034_04640"
     CDS             1128..1832
                     /locus_tag="VN034_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWV59917.1"
                     /translation="MIKVASYNMRKGIGLDRRRDPGRVLSVLSELDADIVALQEADRR
                     FGTRASAIPPHMFADHSDYVPVDLLSGRPYAIGWHGNALLVRKGAEVEESHALHLPTL
                     EPRGAVAATVRIGDTRLRVVGMHLDISGLRRRQQARAILNHVAEGEALPTILMGDCNE
                     WRATGGCLTDFGAQHRLVDTGHSFHSRRPVAKLDRIFATPDLEAVEAGVHRSALAARA
                     SDHLPIWARFKAAADT"
     gene            2119..2457
                     /locus_tag="VN034_04645"
     CDS             2119..2457
                     /locus_tag="VN034_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWV59918.1"
                     /translation="MKLILAIIKPFKLDEVREALTGLGIAGMTVTEVKGFGRQKGQTE
                     IYRGAEYATNMVPKVKIELVCDDALAARVVETLQQSAGTGSIGDGKIFVLDVGQAVRI
                     RTGETGEAAL"
BASE COUNT          477 a          852 c          827 g          402 t
ORIGIN      
        1 tcgcccgcgc gatcgcgcgc ggcatcgtcg cgcgcggctg gcacgaggat tggcccgacg
       61 cgcgccgcca gttcgcctgc ctgccctttc agcatagcga agatctcggc gatcagcgcg
      121 aatcgttacg cctcttcggc cagttcgacg acccgatgtt ccaggattat gcgcaaaagc
      181 atttcgacat cgtcgatcgc ttcggccgct ttccacaccg caacgaagcc ctcggccgcg
      241 cgacccgcgc cgacgaggaa gcggcgatag aagagggcaa gaattggtga tagggcgggc
      301 gcgaaccgga gagcagccat ggccgaattc accgatacgc tgaccgacaa acatatcgcc
      361 ttcatcgaaa agcagccggt cttttttacc gcgaccgctg ccgccgatgg ccggatcaac
      421 ctgtcgccca aaggctatgc cgacagcttc aagatcctgt cggatgcgca ggtcgcctat
      481 ctcgacctcg gcgggtcggg caacgaaacc catgcgcatc ttgccgccga cgggcgcatc
      541 acgatcatgt tctgcgcctt cgatcgcagc gcgctgatcc tgcgcatcta tggccgcggc
      601 cgcccggtgc tgccgcagga cggcgaatgg gacgcgctcg cggcccattt cacgttgctg
      661 cccggcacgc gccagatttt cgtgatcgac gtcgaaagcg tgcagacgag ctgcgggtgg
      721 ggcgtgccga tgatggaatt gcaacacgaa cgcgacacgc tgcagaaata tcaccgccag
      781 gccgaccgcg cgctgtgggt cgaaaaattc aaggagcgcg accgaagcat cgacggcctg
      841 ccgacgcgcc cgaccgaccg cttcatcgcg ggcgacgcct gatgccgctg gtcgagttgg
      901 tgcgtcttcc caatggcgcc gaggccgaac tgctgcgcgg ccggctcgaa agcgccggtg
      961 tccacgcggt ctgcttcgac gcgggcatga atatcgccga aagcgtcggg ctgctcatcc
     1021 cggtgcgcat catggtgctc gatgaggatt tgggcgaagc gcgcgcgttg atggccgagt
     1081 tcgagggcgg cgggaacgga aacgcggcct gagcgcttga aaagcagatg atcaaggtcg
     1141 ccagttacaa tatgcgcaag ggcatcgggc tcgatcgccg ccgcgacccc ggccgcgtgc
     1201 tctcggtgct cagcgagctc gatgccgaca tcgtcgcgct gcaggaggcc gaccgccgtt
     1261 tcggcacccg cgcgagcgcg atcccgccgc acatgttcgc ggatcatagc gattatgtcc
     1321 ccgtcgacct cttgagcggc cgcccctatg cgatcggctg gcacggcaat gcgctgctcg
     1381 tgcgcaaggg cgccgaggtt gaggaaagcc acgcgcttca cctgccgacg ctcgaaccgc
     1441 gcggcgccgt cgcggcgacg gtgcgcatcg gcgatacgcg gctccgcgtc gtcggcatgc
     1501 acctcgacat ttcgggactt cgccgccgcc agcaggcgcg cgcgatcctg aaccatgtcg
     1561 ccgagggcga agcgctgccg acgatcctga tgggcgactg caacgagtgg cgcgcgacgg
     1621 gcggctgcct taccgatttc ggggcgcagc accgcctcgt cgacaccggc cacagcttcc
     1681 acagccggcg ccccgtcgcg aaactcgacc gcatcttcgc cacccccgac ctcgaagcgg
     1741 tcgaggcggg cgttcatcgc agcgcgctcg cggcgagggc atcggatcat ctgccgatct
     1801 gggcgcggtt caaggccgcc gccgacactt aaaccccctc ccgcttgcgg gagggatagt
     1861 gagacttgcg agcttgctcg ctagtcgcag cggggagggc agcgacgcta caggccctcc
     1921 cctgacccct cccgcaagcg ggagggggaa tatgctgcct aaaatttagg caacacttgg
     1981 cctcgcctgc ccaccgaacg cacgatccgg tgcctccgta cctcaaaaac cgtcacaaaa
     2041 tgtcaacttc tgccgccacc gcgcaatttg gcacgcccgt tgcaaagcga catggtgcca
     2101 ttagcaaaag ggggcgtcat gaagctgatc ctggccatca tcaaaccatt caagctcgac
     2161 gaggtgcgcg aggcgctcac cggtctcggc atcgccggca tgaccgtgac cgaggtcaag
     2221 ggcttcggcc ggcaaaaggg gcagaccgaa atctaccgcg gcgccgaata tgccaccaac
     2281 atggtgccga aggtgaagat cgaactcgtc tgcgacgacg cgctcgccgc aagggtcgtc
     2341 gaaacgctgc agcaaagcgc cggcaccggt tcgatcggcg acggcaagat tttcgtgctc
     2401 gacgtgggtc aggccgtgcg catccgcacc ggcgagaccg gcgaggcggc actgtaatga
     2461 cgaaggggga agcaatgaag ttcgcaaaca agattgcggc gggtgccggg gccgcaggcc
     2521 tcgcgctgtt cgccgcgctg cccgcatggg cgcaggaa
//
LOCUS       DATMSU010000182         1630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145173,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000182 DATMSU010000000
VERSION     DATMSU010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145173"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VN034_04650"
     CDS             complement(<1..221)
                     /locus_tag="VN034_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HWV59919.1"
                     /translation="MRIVIIDDSGLRATVLEEGLREAGYDDIHIVPPRGGFVARLERM
                     APDVVLMDLGSPSRDMLEEMLSVSRALARP"
     gene            412..>1630
                     /locus_tag="VN034_04655"
     CDS             412..>1630
                     /locus_tag="VN034_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV59920.1"
                     /translation="MPSITLSHISWSAPDGSPVLSDLDFRFTSERIGLVGRNGVGKST
                     LLALISGRLKPASGSIQLDGTVAELRQTVQVTPDETIADLFGVAQAMALLAKAERGEA
                     DMAELEACDWTLPSRIEEALAEVGLQATPETALNQLSGGQRTRAALAGAVFARPDFLL
                     LDEPTNNLDADGRAALIALLARWGAGAIVVSHDRALLEEMEAIADLTTLGISRHGGNF
                     SSWQARKAVELSAAEQDLANAERHRKEIAKRTQIAAERKQRRDAAGARKGAKGDMPRI
                     LIGMRKDRAEGSGGEAVRLAERLRRESEQIAETARQRIEVVESLTVALPSTAVLPGQR
                     IVTLDHVTAGHDPGAPILKPLSLTINGPERIAITGPNGAGKSTLLHVMAGTLPPLSGK
                     VDRHVASALLDQKV"
BASE COUNT          298 a          558 c          514 g          260 t
ORIGIN      
        1 ggccgggcca gcgcgcggct gaccgacagc atttcttcca gcatgtcgcg gctggggctg
       61 cccaaatcca tcagcacgac atcgggcgcc atgcgttcca gccgcgcgac gaagccgccg
      121 cgcgggggaa cgatatgaat atcgtcatag cccgcctcgc gcagtccttc ctccagaacc
      181 gtcgcgcgca ggccgctgtc atcgatgatg acgattcgca tgtggacctg tgaccctctt
      241 gcccgtttta gtgacggcga tgctgcgccg cgtcaattgg gcgagcgctc caaggttgcc
      301 tgcgccgacc gcctgctgta tcggtgcagg ccaatcttct gaaggatcga accatggaca
      361 tcaccctgat cgctcagccg caacgctatg ctttcaggac tatttttgcc catgccttcg
      421 atcacattat cgcacatcag ctggtccgcg cctgacggtt cgcccgtcct tagcgacctc
      481 gattttcgtt tcacctctga acggatcggc ctcgtcggcc gcaatggcgt cggcaaatcg
      541 acgctgctcg ccctcatttc cggcaggttg aagccggcca gcggcagcat ccagctggac
      601 ggaacagtcg ccgaactgcg ccagacggta caggtcacgc cagacgagac gatcgccgat
      661 ctgttcggcg tggcccaagc gatggccctg ctcgccaaag ccgaacgcgg cgaggccgac
      721 atggccgagc tggaagcatg cgactggacc ctgccctcgc gcatcgaaga ggccttggcc
      781 gaggtcggcc tgcaagctac gcccgaaacc gcgctcaacc aattgtcggg cggacagcgg
      841 acgcgggcgg cgctcgccgg ggcggttttc gcccggccgg acttcctgct gctcgacgag
      901 ccgaccaaca atctcgacgc ggatggccgg gcggcgctca tcgcccttct cgctcggtgg
      961 ggcgcagggg cgatcgtcgt cagccatgac cgcgcgctgt tggaggaaat ggaggcgatc
     1021 gccgatctga cgacgctcgg catcagccgt catggcggta atttctcatc ctggcaggcg
     1081 cgcaaggcag tcgaactgtc cgccgcggag caggatctcg caaacgccga acggcaccgg
     1141 aaggagatcg ccaagcggac gcagatcgcc gccgagcgca aacagcgccg cgacgccgca
     1201 ggcgcccgca agggagcgaa gggcgacatg ccacgcatcc tgatcggcat gcgtaaggat
     1261 cgcgccgaag ggtcaggcgg cgaagcagtg cggcttgcgg aacgtctgcg ccgcgagtcc
     1321 gaacagatag cggaaaccgc gcggcaaagg atcgaagtgg tagaaagcct gaccgtcgcc
     1381 ctcccctcta ccgctgtcct gccggggcag cggatcgtga cgctcgatca tgtcaccgcc
     1441 gggcatgatc ccggcgcgcc gatcctcaag cccctgtccc tcaccatcaa cggccccgaa
     1501 cgcatcgcca tcaccggccc caatggcgcg ggcaaatcca cgctgctcca tgtcatggca
     1561 ggcaccctgc cgccgctgtc cgggaaggtc gatcggcacg tcgccagcgc gctgctggac
     1621 cagaaggtgg
//
LOCUS       DATMSU010000183         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_135790,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000183 DATMSU010000000
VERSION     DATMSU010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135790"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VN034_04660"
     CDS             complement(<1..435)
                     /locus_tag="VN034_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWV59921.1"
                     /translation="MRIGIVAPSTPILPDDAEAVRAVVSLGYPGVELEFDPQCFAVAG
                     HFASEDAQRLTALVTMANRADIDAIWFARGGYGACRIAEAAVAAMEPVARGKAFLGFS
                     DQGNLLGCLYREGFDHVAHGPMVADIRRDGGEATVQRALDWLV"
     gene            591..1970
                     /locus_tag="VN034_04665"
     CDS             591..1970
                     /locus_tag="VN034_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWV59922.1"
                     /translation="MSENKSYFFCGIGGSGMLPLAMIVAARGAAVSGSDRSRDQGRTP
                     EKFAWLEDQGIEFFPQDGSGPKAGQTLVASAAIEDSVPDVAAANVLGLARMTRAELNA
                     ALFNAADKAIGVGGTSGKSTVTGMIGWILESAGRKPTVMNGAVMRNFAGAEMPFASAL
                     VGDAAIYISEVDESDGSIALYAPDVAVVTNISLDHKSLAELHQLFGDFAAKARVAVIN
                     ADDPESAPLLSAGNIVAFGFGESATVRGSDFEALPDGCRFAVHAGGTAYQVKLRMPGR
                     HNAANALAAIAATHAAGTTVAQAVEALASFAGLARRYEVLGQANGVTVIDDFAHNPDK
                     VAATLAAVAELPGRALLFFQPHGYGPLRQMGKELAASFAAGMRADDKLFVCDPVYFGG
                     TVDRSIGSEALVADIVAGGGDAVHMTTRANCGAAMLDEAKPGDRILILGARDDTLTEF
                     GRELVRKLG"
BASE COUNT          384 a          732 c          722 g          348 t
ORIGIN      
        1 gacgagccag tcgagcgcgc gctggaccgt cgcctcgccg ccgtcgcgcc ggatatcggc
       61 gaccatcggc ccgtgcgcga catggtcgaa accctcgcga taaaggcagc cgagcaaatt
      121 gccctgatcc gaaaagccga gaaaggcctt gccgcgcgcc accggctcca tcgccgcgac
      181 ggctgcctcc gcgatccggc acgcgccata gccgccgcgc gcgaaccaga tcgcatcgat
      241 gtcggcgcgg ttcgccatcg tcaccagcgc ggttagccgc tgcgcatctt cgctcgcgaa
      301 atggccagcg acggcgaagc attgcgggtc gaattccagc tcgacgccgg ggtagccgag
      361 gctgacgacc gcacggacgg cttcggcatc gtcgggcagg atgggcgtgc tgggggcgac
      421 gattccgatg cgcatggaca ggactctgat tttccgttcg tgtcgagcga agtcgagaca
      481 cccatcgcca tggcgccaag ccgaggggca tctcgacttc gctcgatgcg aacgggaaag
      541 ggaatgtcgc tccggttgca aacccttcgc caaagccata tcgcgccgcc atgtcggaaa
      601 acaaatccta tttcttctgc ggcatcggcg ggtcgggtat gttgccgctc gcgatgatcg
      661 tcgcggcgcg cggcgcggcg gtttcggggt cagaccgcag ccgcgaccag ggccggacgc
      721 cggagaaatt cgcctggctc gaagatcagg gcatcgaatt tttcccgcag gacggcagcg
      781 ggcccaaggc cggacagacc ctcgtcgcct cggccgcgat cgaggacagc gtgcccgatg
      841 tcgccgccgc gaacgtgctt ggcctcgcgc ggatgacgcg cgccgagctc aacgcggcgc
      901 tgttcaacgc ggcggacaag gcgatcggcg ttgggggaac gagcggcaag tcgaccgtga
      961 cggggatgat cggctggatc ctcgaaagcg cggggcggaa accgaccgtg atgaacggcg
     1021 cggtaatgcg caatttcgcg ggcgccgaga tgcccttcgc gagcgcgctg gtcggcgacg
     1081 ccgcgatata tatcagcgag gtcgacgaaa gcgacggatc gatcgcgctc tacgcgcccg
     1141 acgtcgcggt cgtgacaaac atcagcctcg accacaagag cctcgccgaa ttgcaccagc
     1201 tgttcggcga ttttgcagcc aaggcgcgcg tcgcggtgat caatgccgac gatccggaat
     1261 cggcgccgct gctgtcggcc ggcaatatcg tggccttcgg cttcggcgaa agcgcgacgg
     1321 tgcgcggcag cgatttcgag gcgctgcccg acggctgccg ctttgccgtc catgcgggcg
     1381 gcaccgccta tcaggtgaag ctccgcatgc ccggtcgcca caatgccgcc aacgcgctcg
     1441 ccgccatcgc cgccacccac gcggcgggca caaccgtcgc gcaggcggtc gaagcgctgg
     1501 ccagcttcgc gggtctcgca cgccgctatg aagtgctcgg gcaagcaaat ggcgtcaccg
     1561 tcatcgacga tttcgcgcat aatcccgaca aggtcgccgc gacgctcgcc gccgtcgccg
     1621 aactgccggg ccgcgccctg ctcttcttcc agccgcacgg ctatggtccg ctgcgccaga
     1681 tgggcaagga actggccgcg agtttcgccg ccggaatgcg cgcggacgac aagctgttcg
     1741 tctgtgaccc cgtctatttc ggcggcacgg tggatcgcag catcggcagc gaggcgctgg
     1801 tcgccgacat cgtcgcgggc ggcggcgatg ccgtccatat gacgacccgc gcgaactgcg
     1861 gcgccgcgat gctcgacgaa gcgaaaccgg gcgaccgcat cctgatcctc ggcgcgcgcg
     1921 acgacacgct gaccgaattc gggcgcgaac tcgtgagaaa gctcggctga aatatttcgc
     1981 gcagaggcgc agagaccgca gagaatttgg ttcacgcgga gacgcggagg cgcggagaag
     2041 aaaggaaaat cattggcggc ttcgccgcct tcggcctctc ttcctctgcg atctctgcgc
     2101 ctctgcgcga atctccctct ttccttactt ccgcgtctcc gcgtgaacca aattctctgc
     2161 ggtctctgcg cctctgcgcg aaatat
//
LOCUS       DATMSU010000184        10549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145184,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000184 DATMSU010000000
VERSION     DATMSU010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145184"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3271
                     /locus_tag="VN034_04670"
     CDS             <1..3271
                     /locus_tag="VN034_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59923.1"
                     /translation="VAVEALKVLSDPEAAGAGRDWLAGQTPGIGWLFPEIDHNPRAKA
                     IRAAIKHAPGQTVSLRVNGKSADPLTFEGVSKSADGNVAVSLWRGIEIRDGENRLVAE
                     VKDANGAIIETLERGVHYSITPMRATLIRERSVLVADGVTRPVIAVRLTDRDGKPIRA
                     GLTGDFAVPAPYYPAVEADAQQARQLSGLERAQPLWKIDGDDGVAMIELEPTTASGTL
                     AIDFTFRDDKLVRKQTIETWLDPGDRPWTVVGFATGTLGYNTLDDRMEPVAETLDNLN
                     ADARLALYAKGRVLGKWLMTLAYDSDKEKDDARFGGVIDPRAYYTIYADRNETRYDAA
                     SVRKLYLRLERPQFYAMFGDIETGISEPELARYSRALNGGKAEYRGRNIAATAFVADT
                     PYRFRRDEIQGNGLTGPYQLGAKGILPNSERIVLETRDRLHSERIVDSISLSRHVDYD
                     IDYLAGTIRFRKPVLSRSSNLDPQFIVAEYEVAGVGQRVLNAGGRVSYQSNDEKLRVG
                     ATFIHDEDGNAKTNLGGVDARYRPTIETEVRAELAVSDAKSVNSNTAADGTAKAWLIE
                     AEHHSSNADFLAYVREREAGFGTGQLNRGENGTRKFGFDARVKASRNLSVTGSAWQED
                     YLETSARRRAARALAEYDNGNSVMRAGLTHADDRLTDGTRNRSNIVQLGATQRLFAKR
                     LELDAQTEFALGGKDASVDFPTRHRLGARFAINRDVNLVGSYEIADGDTIKARTARLG
                     FDLTPWSGARLLATANSQEIGEYGPRSFAAYGLSQSLKLGERWSVDVSVDGNRTIGGI
                     SARDVLNVDQPVASGGFLGGNGTLTEDFLAISTGATYRADRWTLTGRAEYRDGELANR
                     YGLTLGGLRQLGEGRALGALFTYAKASGSGTTPTTEVINFELSWAHRPADSRVSWLNK
                     SEFRSDKVRDAVAGQPGPIGGGALTIDGDATSRRLLNSLSVGWTPLGDRGENGMWYER
                     AEIGVFWGTRYNFDRFGADDVKGWSNLIGADFRFNLGEHVDVGASGTVRVGTDADTVS
                     WAGGPTLTLAPMKNANITFGYNFAGFHDRDFEDARFSRSGAYVTFKLKFDQTSFAGLG
                     L"
     gene            complement(3378..4691)
                     /locus_tag="VN034_04675"
     CDS             complement(3378..4691)
                     /locus_tag="VN034_04675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWV59924.1"
                     /translation="MARYSFRLPDIGEGIAEAEIVAWHVKIGERVEEDAQLADMMTDK
                     ATVEMESPVSGIVVELAGEVGDLIPIGSTLAVIETDGDGEGDVEAPPADTPVEEAIEA
                     ETPGADEVSVAEAVPLPLAGGARGGPVEPASVAAETSPPPTPPASGRGDKAVLASPAV
                     RARAKDLGVDLGQVQAEGDRIRHADLDAFLRYSGGQGYHAPGASRARADEPIKVIGMR
                     RKIAENMAASKRAIPHFTYVEEMDVTALEEMRADLNANRGGRPKLTMLPFLIVAICRT
                     IPQFPMINARYDDEGGVVTRYGAVHLGMATQTDAGLMVPVIRDAQDKNVWQLASEITR
                     LAEAARTGKAKVEELTGGTLTVTSLGPLGGIATTPVINRPEVAIIGPNKIVERPVFDG
                     DDIRRAKLMNLSISCDHRVVDGWDAASYVQALKKLIETPVLLFAD"
     gene            complement(4696..5733)
                     /locus_tag="VN034_04680"
     CDS             complement(4696..5733)
                     /locus_tag="VN034_04680"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWV59925.1"
                     /translation="MSAETQTATKTMNMIEAINSAMDVMLERDPNTVVMGEDVGFFGG
                     VFRATAGLQKKHGKTRVFDTPINECGIIGVAVGMGAYGLRPVPEIQFADYIYPGLDQL
                     VSEAARLRYRSANDFICPMTVRTPFGGGIFGGQTHSQSPESIMTHICGVKTVIPSNPY
                     DAKGLLIAAIEDNDPVVFLEPKRIYNGPFSGYYDRPVEPWSKHDASAVPEGHYRIELG
                     KAATVRSGEALTILAYGTMVHVTKTIVEEMGIDAEIIDLRTLLPLDIEAIETSVKKTG
                     RCLIIHEATRTSGFGAELAALVQERCFYHLEAPVERVTGFDTPYPHSLEWAYFPGPVR
                     IATALTKILKD"
     gene            complement(5733..7028)
                     /locus_tag="VN034_04685"
     CDS             complement(5733..7028)
                     /locus_tag="VN034_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HWV59926.1"
                     /translation="MPETPQRPASNLPPLSLHIPEPRYRPGDTPDFGDIDVPAVEATP
                     RPSEATKPDAMRELCYGLVRVLDFDGQAKGQWDPRLSPERLRSMLRNMMLTRAFDDRM
                     FRAQRQGKTSFYMKCTGEEATSVASTMAIDRTDMVFPSYRQQGILITRDYPLIQMMNQ
                     IYSNRGDHLLGRQLPIMYSAPEHGFFSVSGNLATQYPQAVGWAMASASKGDSRIATVW
                     CGEGSSAEGDFHSALTFATVYNAPVIFNVVNNQWAISSFSGFAGSERTTFAARAVGYG
                     IAGLRIDGNDPLAVYAATQWAADRARTNNGPTLIEHFTYRSEGHSTSDDPSAYRAADE
                     ATAWPLGDPIARLRQHLEVIGEWDAERHEAQAKELDDLVKTTQKQSEKLGILGHGMHQ
                     PFETMFEDVFEEMPWHLKEQCAQMLAEQEAKFGPNWKPE"
     gene            complement(7123..8085)
                     /gene="thyA"
                     /locus_tag="VN034_04690"
     CDS             complement(7123..8085)
                     /gene="thyA"
                     /locus_tag="VN034_04690"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HWV59927.1"
                     /translation="MRRQKRERVVSDSIHYELQYLDLMRRIWVEGDERVDRTGVGTRS
                     LFGETMRFSLTDDAIPLLTTKRVYWKTALRELLWFLTGDTNIRSLVAQGVKIWTDWPL
                     DKYRRETGEAISAEDFEARIIADAEFAAAWGDLGPVYGHQWVNWPRYEPAGEGLFRRA
                     SQGHNQIAALIDSLRNNPGSRRHIFTGWNVADLDRMALPPCHMTYQFHVRSDGGLSCL
                     LFQRSCDLGLGFAFNVFEAALLTRMVAEQAGLHAHELVWTGGDVHLYLNHAELVEQQL
                     SRVPEGHPKLRILRRPSSIFDYRFEDFAVEDYAPQAHISAPVAV"
     gene            8201..8629
                     /locus_tag="VN034_04695"
     CDS             8201..8629
                     /locus_tag="VN034_04695"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HWV59928.1"
                     /translation="MTLFEPVGKKPEPSAPAGFIPAGSEDEVARHLLRPLFDDRCETL
                     ILAGFDAFDRLVRFERVDGDTSGRCVIPPRSWRALLEGGVRAVVMAHNHPSGIASPSD
                     ADIGATHDAALFLRTLGIDLVDHLIFVADGHFSFRTAEML"
     gene            8737..8813
                     /locus_tag="VN034_04700"
     tRNA            8737..8813
                     /locus_tag="VN034_04700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8771..8773,aa:Pro,seq:cgg)
     gene            complement(8996..9820)
                     /locus_tag="VN034_04705"
     CDS             complement(8996..9820)
                     /locus_tag="VN034_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWV59929.1"
                     /translation="MSGPVVVSVPHAGRIYPPEILAAARVGRPELERLEDSWCDLIAA
                     RATDAGATVVQALWARAVADLNRGEGQMAPGEVAMPLRMQFSAPGRKERAGLGVIPTR
                     LADCGPLWKRPIDHAALHWRLESFHRPYHAALADALEEARSRFGHAVLIDLHSMPPIP
                     AGQMGHGARIVVGDHFGATADAWLVDRVMTSAERLDTPLSRNQPYAGGHIVRTHGKPA
                     HAIHAVQIEIDRSLYLGPGRTPDSERVARLANWFSDLFAEVGQARPDSETYPEAAE"
     gene            10015..10392
                     /locus_tag="VN034_04710"
     CDS             10015..10392
                     /locus_tag="VN034_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV59930.1"
                     /translation="MIRILLAEDDDVMREYLARALTTAGYHVTAVDRGTAAVPYIDSG
                     TFDLLLSDIVMPEMDGIELAQHTAKAAPQTQVMFITGFAAVSLRAEENVPQAKLLSKP
                     FHLKDLVREVDNLFGRADRSNQQ"
     gene            10464..10538
                     /locus_tag="VN034_04715"
     tRNA            10464..10538
                     /locus_tag="VN034_04715"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10496..10498,aa:Val,seq:gac)
BASE COUNT         1887 a         3448 c         3352 g         1862 t
ORIGIN      
        1 ggtcgcggtc gaggcgctca aggttctgag cgatcccgaa gccgccggcg cgggccgcga
       61 ctggctcgcg ggccagacgc cgggcatcgg ctggctcttt cccgaaatcg accataaccc
      121 gcgcgccaag gcgatccgcg ccgcgatcaa gcacgcgccc gggcagacgg tgtcgcttcg
      181 cgtcaacggc aaatccgccg atccgctgac cttcgagggc gtcagcaaat cggccgacgg
      241 caacgtcgca gtcagcctgt ggcgcggcat cgaaatccgc gacggcgaaa accgccttgt
      301 cgccgaggtg aaggacgcga acggcgctat tatcgaaacg ctggaacgcg gggtccatta
      361 ttcgatcacc ccgatgcgcg cgacgttgat ccgcgaaagg tcggtgctcg tcgccgatgg
      421 cgtcacgcgc ccggtgatcg cggtgcgcct gaccgaccgc gacggcaagc cgatccgtgc
      481 cgggctgacg ggcgatttcg ccgtccccgc gccttattac ccggcggtcg aggccgacgc
      541 gcagcaggca cgccagcttt ctggcctcga acgcgcgcag ccgctgtgga aaatcgacgg
      601 cgacgacggc gtcgccatga tcgaactcga gccgacgacg gcgtcgggca cgctcgcgat
      661 cgatttcacc ttccgcgacg acaagctggt gcgcaagcag acgatcgaaa catggctcga
      721 tccgggcgac cgcccgtgga cggtcgtcgg cttcgccaca ggcacgctcg gttacaacac
      781 gctcgatgac cggatggagc cggtggcgga gacgctcgac aacctcaacg ccgatgcccg
      841 cctcgccctc tatgccaagg gccgcgtgct cggcaaatgg ctgatgacgc tcgcctatga
      901 cagcgacaag gagaaggacg acgcgcgctt cggcggcgtg atcgatccgc gggcctatta
      961 caccatctac gccgaccgca acgagacgcg ttacgacgcc gcctcggtgc gcaagctcta
     1021 cctgcgcctc gagcgcccgc aattctatgc gatgttcggc gatatcgaga cggggatttc
     1081 ggaacccgag ctcgcccgct acagccgcgc gctcaacggc ggcaaggccg agtatcgcgg
     1141 ccgcaatatc gccgcgaccg ccttcgtcgc cgacaccccc tatcgcttcc gccgtgacga
     1201 gattcagggc aacggcctga cgggtccgta ccagctcggt gccaagggca tattgccgaa
     1261 cagcgagcgg atcgtcctcg aaacgcgcga ccgactgcac agcgaacgca tcgtcgacag
     1321 catcagcctg tcgcgccacg tcgattacga catcgactat ctggcgggca cgatccgttt
     1381 ccgcaagccg gtgctcagcc gctcgtcgaa cctcgatccg cagttcatcg tcgccgaata
     1441 tgaggtcgcc ggcgtgggac agcgcgtgct caacgccggc ggccgcgtca gctaccagtc
     1501 gaatgacgag aaactgcgtg tcggcgcgac tttcatccat gacgaagacg gcaacgccaa
     1561 gacgaacctc ggcggtgtgg acgcacgcta tcgcccgacc atcgaaaccg aagtccgcgc
     1621 cgaactcgca gtcagcgatg ccaaatcggt caacagcaac actgctgccg acggcaccgc
     1681 caaggcatgg ctgatcgaag ccgagcacca cagcagcaac gccgacttcc tcgcctatgt
     1741 ccgcgaacgc gaggcaggct tcggtaccgg ccagctcaac cgcggcgaga atggcacgcg
     1801 caaattcggt ttcgacgcgc gcgtgaaagc tagccgcaac ctgtcggtga ccggcagtgc
     1861 gtggcaggaa gattatctcg aaaccagcgc gcgacgccgc gcggcgcgcg cgctcgcgga
     1921 atatgataat ggcaacagcg tcatgcgtgc ggggctgacc cacgccgacg accgcttgac
     1981 cgatggcacc cgcaaccggt cgaacatcgt ccagctcggg gcgacgcagc ggctcttcgc
     2041 caaacggctc gaactcgacg cgcagaccga attcgcattg ggcgggaaag atgcgagcgt
     2101 ggacttcccg acccgccatc gtctcggtgc gcgcttcgcg atcaatcgcg acgtcaatct
     2161 cgtcggcagc tacgagatcg ccgacggcga tacgatcaag gcgcgcaccg cgcggctggg
     2221 cttcgacctg acgccctggt cgggcgcgcg cctgctcgcg accgcgaaca gccaagagat
     2281 cggcgaatat ggcccgcgga gcttcgccgc ctatggcctg tcacaatcgc tgaagctcgg
     2341 cgaacgctgg tcggtcgatg tgtcggtcga cggcaatcgc accatcggcg gcatcagcgc
     2401 gcgcgacgtg ctcaatgtcg accagccggt cgcgtcgggc ggcttcctcg gcggcaacgg
     2461 aacgctgacc gaagatttcc tcgcgatcag caccggcgcc acctaccgcg ccgaccgctg
     2521 gacgctgacg ggccgcgccg aatatcgcga tggcgagctc gccaatcgtt acggcctgac
     2581 gctcggcggc ctccgccagc ttggcgaagg ccgcgcactc ggcgcgctct tcacctacgc
     2641 caaggcgagc ggaagcggca cgacgccgac gaccgaggtg atcaacttcg aattgagctg
     2701 ggcgcatcgc cccgccgatt cgcgcgtctc ttggctcaac aagagcgaat ttcgttcgga
     2761 caaggttcgc gatgcggtcg ccggccagcc cggtcccatc ggcggcggcg cgctgacgat
     2821 cgacggcgac gcgacgagcc gccgcttgct caacagcctg tcggtgggct ggactccgct
     2881 cggcgatcgc ggcgagaacg gcatgtggta cgagcgcgcc gaaatcggcg tcttctgggg
     2941 cacgcgctat aatttcgacc gattcggcgc cgacgacgtc aagggctggt cgaacctgat
     3001 cggcgccgac ttccgtttca atctgggcga gcatgtcgat gtcggcgcgt caggcaccgt
     3061 acgcgtcgga accgacgccg ataccgtcag ctgggccggc ggcccgacgc tgacgcttgc
     3121 gccgatgaag aatgccaaca tcaccttcgg ctataatttc gcgggcttcc acgaccgcga
     3181 tttcgaggat gcgcgcttct cgcgctcggg cgcctatgtg accttcaagc tgaagttcga
     3241 ccagacgagt ttcgcggggt tggggctcta ggccggcccc aaaccccgtt cgcatcgagc
     3301 gaagtcgaga tgcccatcgt aagcgcgcgc cttcggggtg tctcgacttc gctcgacacg
     3361 aacgggtttg ggttggctca gtccgcgaac agcaaaaccg gcgtctcgat cagcttcttc
     3421 aatgcctgta catagctcgc ggcgtcccag ccatcgacga cgcggtggtc gcagctgatc
     3481 gacaggttca tgagctttgc cctgcggatg tcgtcgccat cgaagacggg gcgttcgacg
     3541 atcttgttcg ggccgatgat cgcaacctcg ggacggttga tcaccggggt tgtggcgatc
     3601 ccgccgagcg gcccaagcga agtgacggtc agcgtgccgc cggtgagttc ctcaaccttt
     3661 gccttgcccg tgcgcgcggc ttcggcgagg cgggtgatct cgctagcgag ttgccagaca
     3721 ttcttgtcct gcgcgtcgcg gatcaccggg accatcagcc ccgcgtcggt ctgcgtcgcc
     3781 atgccgagat gcaccgcgcc atagcgcgtc accacgccgc cttcgtcgtc gtagcgcgcg
     3841 ttgatcatcg ggaactgcgg gatcgtgcgg cagatcgcga cgatcaggaa gggcagcatc
     3901 gtcagcttcg gacggccgcc gcgattggcg ttcaaatcgg cgcgcatctc ttcgagcgcg
     3961 gtgacgtcca tttcctcgac gtaagtgaag tgcgggatcg cgcgcttcga cgccgccata
     4021 ttctcggcga tcttgcggcg catgccgatg accttgatcg gctcgtcggc gcgggcacga
     4081 ctggcgccgg gagcgtgata gccctgcccg ccgctataac gcaggaaggc gtcgaggtcg
     4141 gcgtgacgga tgcggtcgcc ctcggcctgc acctgaccga ggtcgacgcc gagatccttc
     4201 gcacgggcgc ggaccgcggg tgacgcaagg acggccttat ctcccctccc gcttgcggga
     4261 ggggtcgggg gagggcttgt ttcggcagca accgaggcgg gctcaacagg ccctccccta
     4321 gcccctcccg caagcgggag ggggactgcc tcggcgaccg aaacctcgtc ggcgcccggc
     4381 gtctccgcct cgatcgcttc ctcgaccggc gtatcggcgg gcggcgcctc gacgtcaccc
     4441 tcgccgtcac catccgtctc gatcaccgcc agcgtcgatc cgatcgggat caggtcgccg
     4501 acctcgcccg ccagttcgac gacgatcccc gagacgggcg attccatttc gacggtcgcc
     4561 ttgtcggtca tcatatcggc aagctgcgcg tcttcctcga cgcgctcgcc gatcttgacg
     4621 tgccaggcga cgatttcggc ctcggcaatg ccttcgccga tgtcgggcag acggaatgaa
     4681 taacgggcca tggcgtcagt ccttcaaaat cttggtcagc gcggtcgcga tgcgcaccgg
     4741 gccggggaaa taggcccatt cgaggctatg cggataaggc gtgtcgaagc cggtgacgcg
     4801 ttcgaccggc gcctcgagat gatagaagca gcgttcctgc acgagcgcgg cgagttcggc
     4861 gccgaagccc gaggtgcgcg tcgcttcgtg gatgatcagg cagcggccgg tctttttcac
     4921 cgacgtctcg atcgcttcga tatcgagcgg caatagcgtg cgcaggtcga tgatctcggc
     4981 atcgatgccc atttcctcga cgatcgtctt ggtgacatgg accatcgtgc cataagcgag
     5041 gatggtgagc gcctcgcccg agcgcaccgt cgcggccttg ccgagttcga tgcggtaatg
     5101 gccttcgggc accgcgctcg cgtcatgctt cgaccaaggc tcgaccgggc ggtcgtaata
     5161 gccgctgaac gggccgttgt agatgcgctt gggctcgagg aagacgacgg ggtcattatc
     5221 ctcgatcgcc gcgatcagca gacccttggc atcataaggg ttcgacggga tcaccgtctt
     5281 cacgccgcag atgtgggtca tgatgctttc gggcgactgg ctgtgcgtct ggccgccgaa
     5341 gatgccgccg ccgaaggggg tgcgcaccgt catcggacag atgaaatcat tcgccgagcg
     5401 atagcgcagc cgcgccgcct cgctgacgag ctggtcgagc cccggataaa tatagtcggc
     5461 gaactggatt tcggggaccg ggcgcagacc gtacgcgccc atgccgaccg cgacgccgat
     5521 gatcccgcat tcgttgatcg gcgtgtcgaa cacgcgcgtc ttgccatgct ttttctggag
     5581 acccgcggtc gcgcggaaca cgccgccgaa aaagccgacg tcctcgccca tcaccacggt
     5641 gttcgggtcg cgttcgagca tgacgtccat ggcgctgttg atcgcctcga tcatgttcat
     5701 ggtcttggtg gcggtttgcg tttcggcgct catcattcgg gcttccaatt ggggccgaac
     5761 ttggcctctt gctcggcgag catctgcgcg cattgttctt tgaggtgcca gggcatctct
     5821 tcaaacacat cctcgaacat cgtctcgaac ggctggtgca tgccatggcc caaaataccg
     5881 agcttttctg actgcttctg cgtcgtcttg accagatcgt cgagttcctt cgcctgcgcc
     5941 tcgtgacgtt cggcatccca ttcgccgatc acctcgagat gctggcgcag ccgcgcgatc
     6001 ggatccccga gcggccatgc ggtcgcctcg tcggcggcgc gataggcgct ggggtcgtca
     6061 gaggtgctgt ggccttcgct gcgataggtg aaatgctcga taagcgtcgg gccgttgttg
     6121 gtgcgtgccc ggtcggccgc ccactgcgtc gcggcataga ccgcgagcgg atcattgccg
     6181 tcgatgcgca gtccggcgat gccgtaaccc accgcgcgcg cggcaaaagt cgtgcgctcg
     6241 ctgccggcaa aacccgagaa gctcgaaatt gcccactggt tgttgacgac gttgaagatg
     6301 accggcgcat tatagacagt cgcaaacgtc agcgccgagt ggaagtcgcc ctcggccgac
     6361 gaaccttcgc cgcaccagac ggtcgcgatg cggctgtcgc ctttcgacgc cgaggccatc
     6421 gcccagccca ccgcctgcgg atattgcgtc gcgaggttgc ccgacacgct gaagaagcca
     6481 tgttcgggcg ccgaatacat gatcggcagc tgacggccga gcagatggtc gccgcggttc
     6541 gaatagatct ggttcatcat ctggatcagc ggatagtcgc gcgtgatcaa tatgccctgc
     6601 tggcgatagc tcgggaaaac catgtcggtc cggtcgatcg ccatcgtcga ggcgaccgag
     6661 gtcgcttcct caccggtgca cttcatgtag aagctggtct tgccctgccg ctgcgcgcgg
     6721 aacatccggt cgtcgaacgc ccgcgtcagc atcatgtttc gcagcatcga gcggagccgc
     6781 tcgggcgaaa gcctgggatc ccactgtccc ttcgcctgcc cgtcgaaatc gagcacgcgg
     6841 acgagaccgt agcaaagctc gcgcatcgca tcgggcttgg tcgcttcgct cgggcgcggg
     6901 gtcgcttcga ccgcgggaac gtcgatatcg ccgaagtccg gcgtgtcgcc gggacgatag
     6961 cgcggttccg ggatatgaag cgacagcggc ggcaaattgc tcgccggacg ctgaggcgtc
     7021 tcaggcatat cttcttccct ttgcaggcgc gaatcagcgc ctagttatat ttcaacgtgg
     7081 cgacatatta ttacggacgc catacgaatg caacgaccgc gctcagaccg cgacaggcgc
     7141 ggaaatatgc gcctgcggcg cataatcctc gaccgcgaaa tcctcgaacc ggtaatcgaa
     7201 aatgcttgaa gggcgacgca ggatgcgcag cttcggatgg ccttccggca cgcgcgacaa
     7261 ttgctgttcg accagctcgg cgtggttcag atacagatgc acgtcgccgc cggtccacac
     7321 gagttcatgg gcatgaaggc ccgcttgctc ggcaaccatc cgcgtgagca gcgcggcctc
     7381 gaagacattg aacgcaaagc ccaggccaag atcgcacgag cgctggaata acaggcacga
     7441 gaggccgccg tcgcttcgca catggaattg ataggtcata tggcagggcg gcagcgccat
     7501 gcggtcgagg tcggcgacgt tccagccggt gaagatgtgg cgccgcgaac ccggattgtt
     7561 gcgaagcgaa tcgatcagcg ccgcgatctg gttgtgtcct tggcttgcgc ggcgaaagag
     7621 cccctcgccc gccggttcgt aacgcggcca gttcacccat tgatgtccgt aaaccgggcc
     7681 gagatcgccc cacgcagcgg cgaactccgc gtcggcgata atccgcgcct cgaaatcttc
     7741 ggcgctgatc gcctcgcccg tttcgcggcg atatttgtcg agcggccaat cggtccagat
     7801 cttgacaccc tgcgcgacga gcgaccggat attcgtatcg ccggtcagga accacagcag
     7861 ctcgcggagc gccgttttcc aatagacgcg cttggtcgtc agcagcggga tcgcatcgtc
     7921 cgtcaacgag aatcgcatcg tctcgccgaa cagcgagcgc gtgccaacgc cggtgcgatc
     7981 gacgcgctcg tcgccctcca cccagatgcg acgcatcagg tcgagatatt gcagttcata
     8041 atggatggaa tcagacacga cacgctcccg cttttgccgt ctcattgcta ggccccgcgc
     8101 cccaagcgga caagggacca gcgggaatgc gactacaaag cctccgatgc ggggcagtcg
     8161 ggcgttggga atcgcgtgcc cggggcgcat ggggccgagg atgaccctgt tcgagcccgt
     8221 cggcaaaaaa ccggagccct ccgcaccggc cggattcata cccgctggat ccgaggatga
     8281 agtagcgcgc catttgttgc gcccgctgtt cgacgatcgc tgcgaaacgc tgattcttgc
     8341 cggattcgac gcgttcgatc gcctcgtccg cttcgagcgt gtcgatggcg acacgtcggg
     8401 ccgctgcgtt atccccccgc gaagctggcg ggctttgctc gaaggcgggg tcagggcggt
     8461 ggtgatggcg cacaaccacc cgtcgggcat cgcctcgccg agcgatgccg acatcggcgc
     8521 gactcacgat gccgcgcttt ttctgcggac cttgggcatc gacctcgtcg atcatctgat
     8581 tttcgttgcg gacggtcatt tcagctttcg aactgccgaa atgctctagt tccggcaatc
     8641 ctcttcaatg tgccgattgg cgcttgaagt tcgcagattg cgcgtctaga ggaccgccct
     8701 gccttcgcgg cgaccccggt cggcgcgcag atcggtcggg gagtagctca gcctggtaga
     8761 gcactgtctt cgggaggcag gggccggagg ttcgaatcct ctctccccga ccaatttcct
     8821 gcatattgca gattctgtat cgtctcttct tgtaagtcgg cgaacaaatc cctaaattca
     8881 aactacgccg ccatggtttt ccccctttcc atcgcggcca gtgtcccgtc agctttggcg
     8941 cggacgcgtc ctccctggac cctggccacc tcgtacttcg gtacgaggtg gttttttatt
     9001 cggctgcctc gggataagtc tctgaatcgg ggcgcgcttg accgacctcg gcaaaaaggt
     9061 ccgaaaacca gttcgcaagg cgcgcgaccc gctctgaatc cggcgtccgt ccagggccaa
     9121 gatacaggct tcggtcgatc tcgatctgca cggcatggat cgcatgagcg ggcttcccat
     9181 gtgtccgcac gatatgcccg ccggcatagg gctggttccg cgacagcggt gtgtccagac
     9241 gctccgccga tgtcatgacc cgatcgacca gccatgcgtc tgccgtcgcc ccgaaatggt
     9301 cgccgacgac gattcgcgcc ccatgcccca tctggccggc gggaatcggg ggcatcgaat
     9361 gcaggtcgat caggaccgca tgtccgaacc ggctccgcgc ttcctccaac gcatcggcca
     9421 gcgcggcatg atagggccgg tgaaacgatt cgagccgcca atgcagcgct gcgtgatcga
     9481 tcggccgctt ccacagcggt ccgcaatcgg cgagccgcgt cggaatcacg ccaagccccg
     9541 cgcgttcttt ccgccccggc gccgaaaatt gcatccggag cggcatcgcg acctcgccgg
     9601 gggccatctg cccttcgccg cgattgaggt cggcgaccgc gcgtgcccac agggcctgca
     9661 caacggtggc acccgcatcg gtggcgcgcg cagcgatcag gtcgcaccag ctatcttcga
     9721 gccgttcgag ctcgggccgg ccaacgcgcg cggcggcgag gatctcgggc ggatagatgc
     9781 gcccggcgtg cggcaccgac accacgaccg ggccgctcac ctcaggccgg ttgacagcaa
     9841 tggcggcggg cggaatggtg cgcgaagaca tgaacgatgg gtggcaaaaa aagccggata
     9901 aatccagcgc tccgcgtcaa tttgttaaat ctctggcgct atgccggaca ggattttgaa
     9961 gggggccgcc cgaccggacg ggcggcgccg aaggaccagg gaacagattt cagcatgatt
    10021 cgcattttgc tcgccgaaga cgatgatgtg atgcgcgagt atctggcgcg agcgctgacg
    10081 accgccggct atcacgtcac ggcggtcgat cgcggcaccg ccgccgtgcc ctatatcgat
    10141 tcgggtacct tcgacctgct cctctcggat atcgtcatgc ccgaaatgga tgggatcgaa
    10201 ctcgcgcagc acaccgcgaa ggcggcgccg cagacgcagg tgatgttcat caccggattc
    10261 gctgcggtgt cgctgcgcgc cgaggaaaat gtgccgcagg ccaagctgct gtcgaaaccc
    10321 ttccacctca aggatctggt gcgcgaggtc gacaatctgt tcggccgcgc ggaccggtcg
    10381 aaccaacaat aaagacgcga ccgcgcgcga agggcttgcc aagccgcgca gaggccttta
    10441 gggggcgcgt caccccaagg gatgggcgtg tagctcagtg gtagagcact gtgttgacat
    10501 cgcaggggtc gcaagttcaa tccttgccac gcccaccatt aaaaacgcc
//
LOCUS       DATMSU010000185        10286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_3599, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000185 DATMSU010000000
VERSION     DATMSU010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3599"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VN034_04720"
     CDS             complement(<1..285)
                     /locus_tag="VN034_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HWV59931.1"
                     /translation="MGTSHIFRPLPLQRGLAIVALSIAPIAASPALAQAGAQGEAEAI
                     VLRPLSFFKVNDLDFGDIIPSNAAGTVTIEPDGSRNRTGGVTLAGDGGEPA"
     gene            400..1323
                     /locus_tag="VN034_04725"
     CDS             400..1323
                     /locus_tag="VN034_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV59932.1"
                     /translation="MSEAAIRIDAVSKLYAGGKQALDNVSFDVPRGTIFGLLGPNGAG
                     KSTLINILAGLVNKTGGSASIWGFDIDADPRNAKYSIGIVPQEIVFDPFFTPYETLEN
                     QAGLYGVPKDKRISDELLAAVHLSDKRDAYARTLSGGMKRRLLVAKAMVHSPPIIVLD
                     EPTAGVDIELRQQLWEYVQSLNDRGVTVVLTTHYLEEAEELCDRIAIINHGKLIANKP
                     TRELVDMAREKIVVVTLDQDVTVLPSHPAFDKVEREGERGLAISYNKDRMNAGEVLAA
                     VNAMGHGIVDVSTREADLEDVFLNLTRAANG"
     gene            1422..3008
                     /gene="nadB"
                     /locus_tag="VN034_04730"
     CDS             1422..3008
                     /gene="nadB"
                     /locus_tag="VN034_04730"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWV59933.1"
                     /translation="MAENLSHDVIIVGSGAAGLTAAIALADHCRVLVLAKGELTGGST
                     AWAQGGIAAVLDAGDTFENHIEDTMIAGGGLNRRETVEFVVENAPHAIERLVEMGVPF
                     NRQAEALHLTREGGHSHRRIVHVDDATGWAVQAALLKAAEAHPNITLLPGQACIDLIT
                     GRHEERYSGSGRVWGVYALDEKSGKVRAHVGRATILASGGAGRVYQFSTAPRGATGDG
                     IAMAWRAGARVSNMEMMQFHPTCLYNLDVKNFLITEAVRGEGGILKHPVTGHRYMPDY
                     DARAELAPRDIVARANDDQIKRSGLDYVHLDISHQDPDFVVGHFPNIYEKLLTLGIDM
                     TKQPIPVVPAQHYTCGGVLIDLDGRTDLPGLYAAGECTESGLHGANRLASNSLLECFV
                     FGEAAAKDIIARWDQLEPPPAIRPWDESRVTDSDEEVVIKQNWTEIRRFMWNYVGIVR
                     TTKRLERAQHRIKMLAGEVGDYYGHFRVTTDLIELRNLLQCAELIVKSALHRKESRGL
                     HYTLDYPDMLPQAVDTVLVP"
     gene            complement(3005..3250)
                     /locus_tag="VN034_04735"
     CDS             complement(3005..3250)
                     /locus_tag="VN034_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWV59934.1"
                     /translation="MINIIGAIISGLIIGALARFFYPGAVEMGWVATILLGIGGSLLA
                     GLATSRGRGDFSRAGCLASVIGAIVLILVGRLLGIGG"
     gene            complement(3247..4452)
                     /locus_tag="VN034_04740"
     CDS             complement(3247..4452)
                     /locus_tag="VN034_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HWV59935.1"
                     /translation="MDILSHSWPAEAETLLGDLVDLRRAIHREPELGLQNPKTLAKIK
                     AALAGLPLEFREGPSTTGLVAILRGPANGRTVLLRGDMDALPLLEDTGLDFTSEINGA
                     MHACGHDTHVAMLVGAAKLLCAARDRLPGTVMFMFQPGEEGHHGARFMLEDGIIDPLP
                     DAAFALHIMPNAPHGIFAGRAGPLLASSDVLSISVKGAGGHASMPHDSIDPIPVACAI
                     VTAIQTMVTRRISVFDPAVVTIAKIAAGTTNNIIPESAEMLGTIRTLSPERRAMVARE
                     LKRLAPAIAEAHGCTAEVHIEEGFPVTICDTRAVAFGQSVVEQTFGEQAWLTMDNPVM
                     GAEDFSYVLEKAPGAMFWLGASEAGSDWRQCCGLHSNHMVLDESVMARGAALHAALAE
                     RFLNEGFNA"
     gene            complement(4433..5827)
                     /locus_tag="VN034_04745"
     CDS             complement(4433..5827)
                     /locus_tag="VN034_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV59936.1"
                     /translation="MQSPLLPEAPTAARYALTASLFILLQHVFVALAVLGSGARPKID
                     NAFLLFPIRELAMQSDVSPLVVIAGFAVALLISTLLVILSLRRAAFLGRGGGLALVAM
                     VPTLQILAVLALTVLWRRPATPEPATAPETGSDAIAPRQTAIGVLAGMALIILAVAVS
                     TVTFGSYGWGLFVMTPLLVGVTTGYIINRGKRRSVGETFLGVVAAGTLGTAALLMLAL
                     EGFMCIVLIAPLAIVLAGIGGEIGRAAARQWHPHQPFYSIAVLPLIFALEAAMPPELP
                     IVTRGSIVIAAPPDAVWRSLTADRPVTEAPGLVGMAGLAYPVASRIDGEGLGAHRVGI
                     FSTGPADERITIWRPGRTLAFRVVHQPPAMEEMSPYRNLDTPHLIGYFDTGETRFDLV
                     PLADGRTRLIATADHVLRIDPVLYWGPIASWAIGRNVKRVLTDVRNDAERGAEMARQR
                     APVSAQSPHGHPIA"
     gene            complement(5839..6795)
                     /locus_tag="VN034_04750"
     CDS             complement(5839..6795)
                     /locus_tag="VN034_04750"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HWV59937.1"
                     /translation="MRSDQDPHAVNFLIDEPGPDPRQGRVRADAPSIAWNGGMAVTAL
                     VAAPFFATPSAVLLFFVTTGAALLLGHSVGYHRMMIHGSFKAPRWFARTLVWFGAFVG
                     MAGPYGIVRAHDTRDWAQRQSDCHPFLAHKTHILRDAWWQIYCRLDLDRPPRFDFAEL
                     DRDPFNRWLEATWRWQQLPIALLFFAIGGWGWVVWGVAARVAVANHGHWLVGHLAHNR
                     GPQHWTIDAHGVQAFDVPWAAIPTMGEAWHNNHHAYPGSARIGLHPGQSDWGYAFIRL
                     LERMRLARDVRTPDTMGLRDGLSRVDPGDPLQPPAALAGAAL"
     gene            6884..7201
                     /locus_tag="VN034_04755"
     CDS             6884..7201
                     /locus_tag="VN034_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWV59938.1"
                     /translation="MERVFQALASTPRRRILAYLAHAELSAGDIAARFEMSKPAVSQH
                     LSVLENAGLIVSEKRGQFVFYRLVPEALTNVLNGYVQEVCPVSRPLKAESRALAEKRR
                     EES"
     gene            complement(7198..8373)
                     /locus_tag="VN034_04760"
     CDS             complement(7198..8373)
                     /locus_tag="VN034_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV59939.1"
                     /translation="MTAFDDWRVRSPYYDETHEALAQSVRRFVAREIAPHIDRWEAEG
                     ELPRELHKKAAEAGILGLRYPEQYGGHSTGFDNFHGLVLTEELAAVGAGGLGASLMTH
                     GIGLPPILALGSDELKQRVAPPVLAGEKIIALGITEASGGSDVANLKTTAVKDGDSYI
                     VNGGKMFITSGMRADWLTCAVRTGGPGAAGISLLLIDMGAPGVERTRLDKMGWRCSDT
                     AAIHFSDVRVPAENLIGPENGGFIGIMRNFNSERLGMAMGCCAYARVAMAEAAEWAQQ
                     RETFGKPLVGHQSIRIKLADMERQIEATQAWVDLCAWQVKNGKDRPADFAMLKVQATR
                     MLEAVAREAAQILGGASYITGSKVERIYREVRVNAIGGGSEEIMLDLAGRQLFGGRR"
     gene            8466..9737
                     /locus_tag="VN034_04765"
     CDS             8466..9737
                     /locus_tag="VN034_04765"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWV59940.1"
                     /translation="MNKSQLLAGLGALALISTPAIAQKSPEAVADAALRKAPVFDGHN
                     DVPWALRGRESNMINGFDFEDTTDTAKPDATPPEIAMHTDLQRLRKGRVGAQFWSVYV
                     PSNPDEPLAVRQTIEQIDVMKRLVARYPDDLMLASTSAELEKAMKAGKIAGMLGMEGG
                     HSIGSSLAVLRQMYDLGARYMTLTHSKNLSWADASTDAPAHDGLTDFGRQVVREMNRI
                     GMIVDLSHVSEATMKDALETSHAPVMFSHSGVRAINPHPRNVPDSVLPAVKANGGIVM
                     VVLLPGFLDADVRAQGLARTAEQARLKAMYPGDPAAEKAALKAWDAANPAPTTDVAKV
                     ADHIDHLKKTIGVDHIGLGGDYDGMDSAPVGMEDVSGYPALFVELAKRGYSQAELEKI
                     ASGNMLRVLKAVEAYAASQKGQPPIETPVAK"
     gene            complement(9741..10142)
                     /locus_tag="VN034_04770"
     CDS             complement(9741..10142)
                     /locus_tag="VN034_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV59941.1"
                     /translation="MKDRVRTALRWLLALAYAYAGWRHLAAPAPFVAITPPWVPQPQL
                     VVAATGIAEIAGAVGLMIPATRKAAGWLLALYALSVWPANFHHALANVAIGGETLGWW
                     YHGPRLAAQPLFIWWALWASGAIDWPFGRRR"
BASE COUNT         1830 a         3272 c         3561 g         1623 t
ORIGIN      
        1 cgcgggttcg ccgccgtcgc ccgcgagcgt cacgccgccg gtacggttcc gcgatccgtc
       61 gggttcgatc gtgacggtcc ccgcggcgtt cgacgggatg atgtcgccga agtcgaggtc
      121 gttcaccttg aagaaggaaa gcgggcgaag cacgatcgct tcggcctcgc cctgcgcgcc
      181 cgcctgcgcc agcgccggcg aggcggcgat gggcgcgatc gagagcgcaa cgatggcgag
      241 cccgcgctgc agcggcagcg ggcgaaagat gtgactggtc cccacaggcc tcgtcctaag
      301 ggcaagattc caatttagcg ttaacgcgcg cgggtcagca gaaaatctat cggcgatgat
      361 ggctttcacg cgggctgacg cgcggctatg gaggcggcca tgagtgaagc cgctatccgc
      421 atcgacgccg tctccaaact ctatgccggc ggcaagcagg cgctcgacaa tgtcagtttc
      481 gatgtaccgc gggggacaat tttcggcctc ttgggcccga acggagcggg caagtcgacg
      541 ctgatcaaca tcctcgccgg gctggtcaac aagacgggcg gcagcgcgag catctggggg
      601 ttcgacatcg acgccgatcc caggaacgcc aaatattcga tcggcatcgt gccgcaggag
      661 atcgtcttcg atcccttttt cacgccctat gagacgctgg aaaatcaggc gggcctctat
      721 ggcgtgccga aggacaagcg catttcggac gaactgcttg ccgcggtgca tctgtcggac
      781 aagcgcgacg cctatgcgcg cacattgtcg ggcggcatga agcgccgcct gctcgtcgcg
      841 aaagcgatgg tccattcgcc gccgatcatc gtcctcgacg agccgaccgc gggcgtcgac
      901 atcgaactca ggcagcagct ttgggaatat gtgcagtccc tcaacgaccg cggcgtgacc
      961 gtcgtgctga cgacgcacta tctggaggaa gccgaggagc tttgcgatcg gatcgcgatc
     1021 atcaaccatg gcaagttgat cgcgaacaaa ccgacgcgcg agctggtcga catggcgcgc
     1081 gagaagatcg tcgtggtgac gctcgatcaa gatgtcaccg tgcttccgag ccacccggcc
     1141 ttcgacaagg tcgagcgcga gggcgagcgc ggcctcgcga tcagctacaa caaggaccgg
     1201 atgaacgcgg gcgaggtgct cgcggcggtc aatgccatgg ggcacgggat cgtcgacgtc
     1261 tcgacgcgcg aggccgatct cgaggatgtg ttcctcaacc tgacgcgcgc ggcgaatggc
     1321 tagcctctct gtccgttcgc atcgagcgaa gtcgagatgc ccatcggcgt ggctcaaggg
     1381 cgcggggtgt ctcgacttcg ctcgacacga acggaaattg aatggctgaa aatctttccc
     1441 acgacgtcat catcgtcggt tccggcgctg cagggctgac cgccgcgatc gcgctcgccg
     1501 atcattgccg cgtgctcgtg ctcgcgaagg gcgagctcac cggcgggtcg accgcatggg
     1561 cgcagggcgg gatcgccgcg gtgctcgacg ccggcgacac cttcgaaaat catatcgagg
     1621 acacgatgat cgcgggcggc ggattgaacc ggcgcgagac ggtcgagttc gtcgtcgaga
     1681 acgcgccgca cgcgatcgaa cgtctcgtcg aaatgggtgt gccgttcaac cggcaggccg
     1741 aggcgctgca cctgacgcgc gagggcggcc attcgcatcg ccggatcgtc catgtcgacg
     1801 acgcgactgg ctgggcggtg caggcggcac tcctgaaggc cgccgaggcg catccgaaca
     1861 tcacgctgct gccgggacag gcgtgcatcg acttgatcac cggccggcac gaggagcgct
     1921 attcgggctc gggccgcgtc tggggcgtct atgcgctcga cgagaagagc ggcaaggtgc
     1981 gtgcgcatgt cggccgcgcg acgatcctcg cgagcggcgg ggcgggacgc gtctatcaat
     2041 tctcgaccgc gcccagagga gcaacgggcg acggcatcgc gatggcgtgg cgcgcgggcg
     2101 cgcgcgtgtc gaacatggag atgatgcagt tccacccgac ctgcctctac aatctcgacg
     2161 tcaagaattt cctgatcacc gaggcggtgc gcggcgaggg cggcatattg aagcaccccg
     2221 tcaccgggca tcgctatatg cccgattatg acgcgcgcgc cgaacttgcg ccgcgcgata
     2281 tcgtggcgcg cgcgaacgac gaccagatca agcggtcggg gctcgactat gtccatctcg
     2341 acatcagcca tcaggacccc gatttcgtcg tgggccattt cccgaacatc tacgaaaagc
     2401 tgctgacgct cgggatcgac atgaccaagc agccgatccc ggtggtgccc gcgcagcatt
     2461 atacctgcgg cggagtgctg atcgacctcg acgggcgcac cgacctgccc gggctatacg
     2521 cggcgggcga atgcaccgag agcgggctcc acggcgcgaa tcgcctcgcg tcgaacagct
     2581 tgctcgaatg tttcgttttc ggcgaggcgg cggcgaagga catcatcgcg cgctgggatc
     2641 agctcgaacc gccgccggcg atccgaccgt gggacgaaag ccgcgtcacc gattcggacg
     2701 aagaggtggt gatcaagcag aactggaccg agatccgacg cttcatgtgg aattatgtcg
     2761 gcatcgtccg caccacgaag cggctcgagc gcgcgcagca ccgcatcaag atgctggcgg
     2821 gcgaggttgg cgactattac ggccatttcc gtgtcacgac cgacctgatc gaactgcgca
     2881 acctgctcca gtgcgccgaa ctgatcgtga agagcgcgct ccaccgcaag gaaagccgcg
     2941 ggctgcacta tacgctcgat tatcccgata tgctgccgca ggcggtcgac acggtgctgg
     3001 tgccttaacc gccaatcccc agcagtcgtc cgacaagaat tagcacgatc gcgccgatca
     3061 ccgaggcgag gcagcccgcg cggctgaaat cgccgcgccc gcgcgatgtc gcgaggccgg
     3121 cgaggagcga gccgccgatg ccgagcagga tcgtcgcgac ccatcccatc tcgaccgcgc
     3181 cgggatagaa gaagcgcgcc agcgcgccga tgatcaggcc cgagatgatc gcgccgatga
     3241 tgttgatcat gcgttgaatc cttcgttgag aaagcgttcg gcgagcgccg cgtggagcgc
     3301 cgcgccgcgc gccatcacgc tctcgtcgag caccatatgg ttactgtgga gcccgcagca
     3361 ctggcgccag tcgctgcccg cctcgctcgc gccgagccag aacatcgcgc cgggggcctt
     3421 ttcgaggacg taggagaaat cctcggcgcc catcaccgga ttgtccatcg tcagccaggc
     3481 ttgctcgccg aaggtctgct cgaccaccga ctggccgaag gcgaccgcgc gcgtgtcgca
     3541 gatggtgacg ggaaagcctt cctcgatatg cacttcggcg gtgcaaccat gcgcctcggc
     3601 gatcgcgggg gcgaggcgct tcagttcgcg ggcgaccatc gcgcgccttt ccggcgacag
     3661 cgtgcggatc gtgccgagca tttcggcgct ttcggggatg atattgttcg tcgtgcccgc
     3721 cgcgatcttc gcgatggtga cgaccgcggg gtcgaagacc gagatgcggc gcgtcaccat
     3781 cgtctggatc gcggtgacga tcgcgcaggc gacggggatc gggtcgatgc tgtcgtgcgg
     3841 catcgatgca tggccgcccg cgcccttgac gctgatcgag agcacgtccg acgaggcgag
     3901 cagcggaccc gcacgcccgg cgaagatgcc gtgcggcgca ttgggcatga tgtggagcgc
     3961 gaaggcggcg tcgggcagcg ggtcgatgat cccgtcctcc agcatgaagc gcgcgccatg
     4021 atgaccttcc tcgccgggct ggaacatgaa catcacggtg ccggggagcc ggtcgcgcgc
     4081 ggcgcagagc agcttcgccg cgccgacgag catcgcgaca tgcgtgtcgt gcccgcatgc
     4141 atgcatcgcg ccgttgatct ccgacgtgaa atcgagcccg gtgtcctcga gcaagggcag
     4201 cgcgtccatg tcgccgcgca gcagcacggt gcgcccgttg gcggggccgc gcaggatcgc
     4261 gacgaggccg gtcgtcgacg gcccttcgcg aaattcgagc ggcagtccgg cgagcgcggc
     4321 cttgatcttc gcgagcgtct tcgggttctg caggcctaac tccggctcgc ggtgaatagc
     4381 gcggcgaagg tcgacgaggt cgcccaagag ggtttcggcc tcggcgggcc agctatgcga
     4441 taggatgtcc atgcggcgac tgtgccgata cgggggcgcg ctgtcgagcc atctcggcgc
     4501 cgcgttcggc atcgttccgc acgtcggtca gcacccgttt gacattccgc ccgatcgccc
     4561 agctcgcaat cgggccccag tagagcaccg ggtcgattcg caggacatga tcggccgtag
     4621 cgatgagccg cgtccgccca tcggccagcg gcacgaggtc gaagcgcgtt tcccccgtgt
     4681 cgaaatagcc gatcaggtgc ggcgtatcga gattccggta cgggctcatt tcttccatcg
     4741 ccggcggctg gtggaccacc cggaaggcga gcgtccggcc gggtcgccag atggtgatgc
     4801 gctcgtcggc gggcccggtg gaaaaaatgc cgacgcgatg cgcgcctagg ccttcgccgt
     4861 cgatacggct cgcgacggga taggcaagcc ccgccattcc gacgagcccg ggcgcctcgg
     4921 ttaccgggcg gtcggcggtc aggctgcgcc acacggcatc gggcggggcg gcaatgacga
     4981 tgctgccgcg cgttacgatc ggcagttcgg gcggcatcgc cgcttcgagt gcgaagatga
     5041 gtggcaggac ggcgatactg tagaacggct ggtgtggatg ccattgtctg gcagcggcgc
     5101 gcccgatctc gccgccgatt cccgccagca cgatcgccag cggcgcgatc agaacgatgc
     5161 acatgaagcc ttcgagcgcg agcatcaaaa gcgcggcggt gccgagggtt cccgccgcga
     5221 cgacgccaag aaatgtctcg ccgacgctgc gccgcttgcc gcggtttatg atgtaaccgg
     5281 tggtgacgcc gacaaggagc ggcgtcatca cgaacaggcc ccagccatag gagccgaagg
     5341 tcacggtcga aaccgcgacc gcgagaatga tcagcgccat gcccgcgagg acgccgatcg
     5401 cggtctgacg gggtgcgatc gcgtcgctgc cggtttctgg cgccgtcgcc ggctccgggg
     5461 tggcgggtcg gcgccacagg accgtcagcg ccagcaccgc cagaatctgg agcgtgggaa
     5521 ccatcgcgac caacgccagc ccgccgccgc ggccgagaaa agccgcgcgc ctcagcgaca
     5581 ggataacgag cagggtggat atcagaagcg cgaccgcaaa accggcgatc acaacaaggg
     5641 gcgaaacgtc tgactgcatc gcaagttcgc ggatggggaa cagcaggaaa gcgttatcga
     5701 tcttcggacg cgcgccgctg ccaaggacgg cgagcgcaac gaaaacgtgc tggagcagga
     5761 tgaaaaggga cgcggtcagc gcatagcggg ccgctgtcgg cgcttcgggg agcagcgggc
     5821 tttgcaataa tcgtcttatc atagcgcggc cccggcaagc gcggcgggcg gctggagcgg
     5881 gtcgcccgga tcaacccgac tcagcccatc gcgcaagccc atcgtgtcgg gcgtgcgaac
     5941 gtcccgcgcg agcctcatgc gttcgagaag gcggatgaaa gcataacccc agtcgctttg
     6001 ccccggatgc aggccgatgc gcgccgaacc cggataggcg tggtggttat tgtgccatgc
     6061 ctcgcccatc gtcgggatcg cggcccatgg gacgtcgaac gcctgcacgc cgtgcgcatc
     6121 gatggtccag tgctgcgggc cgcgattgtg cgcgagatgg ccgacgagcc agtgcccgtg
     6181 attcgcgacc gcgacgcgcg ccgcgacgcc ccagacgacc cagccccagc cgccgattgc
     6241 gaaaaagagg agcgcgatgg ggagttgctg ccagcgccat gtcgcctcga gccagcggtt
     6301 gaaggggtcg cggtcgagtt cggcgaaatc gaaacgcggc gggcggtcga ggtcgaggcg
     6361 gcaatagatt tgccaccagg cgtcgcgaag gatgtgggtt ttatgcgcga ggaacgggtg
     6421 acaatcggac tgtcgctggg cccagtcgcg ggtgtcgtgc gcgcggacga tgccataagg
     6481 ccccgccatg ccgacgaagg cgccgaacca gacgagcgtg cgtgcgaacc agcgcggcgc
     6541 cttgaagctg ccgtggatca tcatgcggtg ataaccgacg ctgtggccga ggagcagcgc
     6601 cgcgccggtc gtgacgaaga agagcagcac cgccgagggg gtggcgaaga aaggggccgc
     6661 gacgagagcg gtcaccgcca tgccgccatt ccaggcgatg ctcggcgcgt cggcgcgcac
     6721 gcggccctgg cgcggatcgg ggcccggctc gtcgatcagg aaattgacgg catggggatc
     6781 ctgatcgctg cgcatcggcc gactccttat agaaagaata taagtaatta cttaaataaa
     6841 ttattgcttg tcaagtcggg tgaggatgcc catttcggga cttatggaac gggtttttca
     6901 ggcattggca tccacaccgc ggcgcaggat tctcgcctat ctggcgcatg ccgaactcag
     6961 cgcgggcgac atcgcggcgc gtttcgagat gagcaagccg gcggtgtcgc agcatctgtc
     7021 ggtgctggaa aatgcggggc tgatcgtcag cgaaaagcgc ggccagttcg ttttctatcg
     7081 cctcgtcccc gaggcgctca ccaatgtgct caacggctat gtgcaggaag tctgcccggt
     7141 gtcgcggccg ctgaaggccg aatcgcgcgc gctggccgaa aagcggcgcg aagagagcta
     7201 gcgcctgccg ccgaagagct gccgccccgc aagatccagc atgatttcct cgctgccgcc
     7261 gccgatcgca ttgacgcgga cttcgcggta gatgcgttcg accttgctgc cggtgatgta
     7321 gctcgcaccg ccgagtatct gcgccgcctc gcgcgcgacc gcctcgagca tgcgcgtcgc
     7381 ctgcaccttg agcatcgcga agtcggcggg gcggtccttg ccgttcttga cctgccacgc
     7441 gcagaggtcg acccacgcct gcgtagcttc gatctggcgt tccatgtcgg cgagcttgat
     7501 gcggatcgac tgatggccga cgagcggctt gccgaacgtc tcgcgttgct gcgcccattc
     7561 ggccgcttcg gccatcgcga cgcgggcata agcgcagcag cccatcgcca tgccgagccg
     7621 ctcgctgttg aagttgcgca tgatgccgat gaagccgccg ttttcggggc cgatgaggtt
     7681 ttcggcgggg acgcggacgt cgctgaaatg gatcgcggcg gtgtcgctgc accgccagcc
     7741 catcttgtcg agccgcgtgc gctcgacgcc gggcgcgccc atgtcgatca gcagcaacga
     7801 gatgcccgcc gcgcccggcc cgccggtgcg gacggcgcag gtcagccagt cggcgcgcat
     7861 gccgctggtg atgaacatct tgccgccgtt gacgatgtag ctgtcgccat ccttcaccgc
     7921 ggtcgtcttg aggttcgcga cgtcgctgcc gccgctcgcc tcggtgatgc cgagcgcgat
     7981 gatcttctcg cccgcgagca ccggcggggc gacgcgttgt ttgagttcgt ccgaacccag
     8041 cgcgaggatc gggggcaggc cgatgccgtg cgtcatcagc gaggcgccga gcccgccagc
     8101 gccgaccgcg gcgagctcct cggtcaatac gaggccgtgg aaattgtcga agccggtgct
     8161 gtgcccgcca tattgttcgg ggtagcgcag cccgaggatg ccggcttcgg ctgccttttt
     8221 gtggagttcg cgcggcaatt cgccctcggc ctcccagcgg tcgatatgcg gcgcgatctc
     8281 gcgcgcgacg aagcggcgga cgctttgcgc cagcgcttcg tgggtttcgt cataataggg
     8341 cgagcggacg cgccagtcgt cgaaggctgt catggtccga tgctgcgcgg ggttgacgga
     8401 cacgtcaagt aggagaggtc aggcgaggct tgcgccgcga cgacaaggac ttaaggtcgc
     8461 gcgccatgaa caagtcgcaa ctgctcgccg gtctcggcgc tctcgccctc atctccaccc
     8521 ccgccatcgc gcagaagtcg cccgaagccg ttgcggatgc cgcgctcagg aaggcgccgg
     8581 tgttcgacgg gcacaatgac gtgccgtggg cgctgcgcgg gcgcgaaagc aatatgatca
     8641 acggcttcga tttcgaggac acgaccgata ccgcgaaacc cgacgcgacc ccgcccgaaa
     8701 tcgcgatgca caccgacctt cagcggctac gcaaggggcg ggtcggcgcg caattctggt
     8761 cggtctatgt gccgagcaat cccgatgagc cgctcgcggt gcggcagacg atcgagcaga
     8821 tcgacgtgat gaagcggctc gtggcacgct atccggacga cctcatgctc gcctcgacct
     8881 cggccgagct cgaaaaggcg atgaaggcgg gcaagatcgc cgggatgctc gggatggagg
     8941 gcggccattc gatcggctcg tcgctcgccg tgctgcgcca gatgtacgat ctcggcgcgc
     9001 gctatatgac gctgacgcat tcgaagaatc tgagctgggc ggatgcttcg accgacgcgc
     9061 ccgcgcacga cgggctcacc gatttcggcc gccaggtggt gcgcgaaatg aaccgcatcg
     9121 ggatgatcgt cgatctgagc catgtcagcg aagcgacgat gaaggacgcg ctcgaaacct
     9181 cgcatgcgcc ggtgatgttc agccattcgg gcgtgcgcgc gatcaatccg catccgcgca
     9241 acgttcccga cagcgtgctg cccgcggtga aggcgaacgg cgggatcgtg atggtcgtgc
     9301 tgctgcccgg cttcctcgac gccgatgtcc gcgcgcaggg ccttgcgcgc accgccgaac
     9361 aggcgcggct gaaggcgatg tatccgggcg acccggcggc ggagaaagcg gcgctgaagg
     9421 cgtgggacgc agccaatccg gcaccgacga ccgatgtcgc gaaggtcgcg gaccatatcg
     9481 atcatctcaa gaagacgatc ggcgtcgatc atatcgggct gggcggcgac tatgacggga
     9541 tggattcggc gccggtgggg atggaggatg tgtcgggcta tccggcgctt ttcgtcgagc
     9601 tggcgaagcg cggctacagc caggccgagc tcgaaaagat cgcgagcggc aacatgctgc
     9661 gcgtactgaa ggcggtcgag gcctatgccg cgagccagaa gggccagccg ccgatcgaaa
     9721 cgccggtcgc gaaataggac tcagcggcgg cggccgaacg gccagtcgat cgcgccgctc
     9781 gcccagagcg cccaccagat gaacagcggc tgcgcggcga ggcgcgggcc atgataccac
     9841 cagccgagcg tctcaccgcc gatcgcgaca ttggcgagcg catggtggaa attggcgggc
     9901 cagaccgaga gcgcgtagag cgcgagcagc cagccagccg ctttccgcgt cgcgggaatc
     9961 atcagcccga ccgcgcccgc gatttcggcg atgccggtcg cggcgacgac gagctggggc
    10021 tgcggcaccc aaggcggggt gatcgcgaca aagggcgcgg gcgccgcgag atggcgccag
    10081 cccgcatagg cataagcgag cgcgagcagc cagcgcagcg cggtacggac gcggtccttc
    10141 accgcccgat cgcgccgagc atcgcctcga tgctggtgaa cttctcgcgc gggttgccgt
    10201 cgagcgccgc cttgacctcg gcctcctcga tccggcgcca gtcgcggaag gtgacggggg
    10261 tgacgtcgcg gctagcgagc agcgcg
//
LOCUS       DATMSU010000186         4319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_98037, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000186 DATMSU010000000
VERSION     DATMSU010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98037"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VN034_04775"
     CDS             complement(<1..349)
                     /locus_tag="VN034_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWV59942.1"
                     /translation="MTDPERIPVIIGVGQVNDRPEDPDQGLDSLGLMIAALKIADADA
                     GGGLLSDLDSLAIVDQISFHHLGKLCEPLAAAIGADPVINYQSAAPHGDTPVRLLNEA
                     ANRIGAGEIKLAAV"
     gene            complement(358..1152)
                     /locus_tag="VN034_04780"
     CDS             complement(358..1152)
                     /locus_tag="VN034_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV59943.1"
                     /translation="MTDTTILTECRGHILIVTINRPEARNAVNAAVHVGIGTALEEAE
                     NDPEIRAVVITGAGDKSFCAGADLVALSRGESLQPHDPAQQAWGFAGMVSHPISKPII
                     AAVNGFAFGGGCEIALMSDIIIAADHAQFGLPEVKVGLFAAAGGAFRLAQQLPRKLAM
                     EYMLTGDPIPAARAAEFGLVNHVVPLADLLPTAIALAEKIAANAPLSVQASKRVALGI
                     DEGRIAADAPYWEHNTRERGVLMRSEDAREGPLAFAQKRKPEWKAR"
     gene            complement(1297..1947)
                     /gene="fsa"
                     /locus_tag="VN034_04785"
     CDS             complement(1297..1947)
                     /gene="fsa"
                     /locus_tag="VN034_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HWV59944.1"
                     /translation="MKFFADTAEIADIQELAATGLLDGVTTNPSLIAKSGRDFKEVTK
                     EICALTTGPVSAEVVALDHATMMKEAEVLRKIAPNVCIKVPLTIDGLKTCKALTGDGT
                     MVNVTLCFSAAQALLAAKAGATFVSPFVGRHDDNGFDGMQLISDIRLIYDNYGYETEI
                     LVASVRHGIHVLEAAKIGADVMTAPPSVIKGLFKHILTEKGIEGFLADWAKTGQSI"
     gene            2160..3521
                     /gene="radA"
                     /locus_tag="VN034_04790"
     CDS             2160..3521
                     /gene="radA"
                     /locus_tag="VN034_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWV59945.1"
                     /translation="MAKAKRQYVCQNCGSATYRWQGQCADCGEWNTLIEEVAETVFSA
                     KHDLSRGGRTLALETLDAESKMPDRMLCGIAEFDRALGGGFVAGSATLIGGDPGIGKS
                     TLLLQAAGRLAKAGKSVVYISGEEAAAQVRLRAQRLGLGDAPVALASATSVRDILATL
                     DKQTADFVVIDSIQTMHSDLIDSAPGTVSQVRASAQELIRYAKDSDAAIVLVGHVTKD
                     GTIAGPRVLEHMVDTVLAFEGERSHQYRILRAVKNRFGGTDEIGVFAMGEEGLGEVSN
                     PSSLFLTDRSRDVPGSVVFPALEGTRPVLVEVQALTVRLASGATPRRAVVGWDSGRLA
                     MVLAVLEARCGLQMGAAEVYLNIAGGYRLTDPAADLAVAAALISAFSERPVPADAIVF
                     GELSLSGEVRQVSHDGLRLREAAKLGFSRGWGPKGMKAVGGISVTGFERLGELVDLML
                     GRD"
     gene            3532..4068
                     /locus_tag="VN034_04795"
     CDS             3532..4068
                     /locus_tag="VN034_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWV59946.1"
                     /translation="MGSLTALDIVVLVLVGGGAVLGFSRGLVQEVTTLLAWILAIAAV
                     RFFHPLVTDLLAGWVGTEGGAAALAFVLLFGGVFALAKWGSRAMGQRSRASLVGGFDR
                     GLGAGFGAVKGLLIATIGFMIVTLLYDIGYGNTQRPVWMTDSRTYPALNATSGAMSKV
                     IAERRAEAREAEAEADAS"
     gene            4065..>4319
                     /locus_tag="VN034_04800"
     CDS             4065..>4319
                     /locus_tag="VN034_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HWV59947.1"
                     /translation="MSAPLYNRDILALAVATADYLPLADARHRISKRAPLCGSAIILD
                     LDTDDAGRVTRVGMHVEACALGQASAALLARHAPGLGLADI"
BASE COUNT          662 a         1331 c         1556 g          770 t
ORIGIN      
        1 ccaccgccgc caatttgatc tcgcccgcgc cgatgcggtt cgccgcttcg ttgagcaggc
       61 gcaccggggt gtcgccgtgc ggcgcggcgg actgatagtt gatcacagga tcggcgccga
      121 tcgcggcggc gagcggttcg cagagcttgc ccagatggtg aaagctgatc tggtcgacga
      181 tcgcgaggct gtcgaggtcg gacagcaagc caccgcccgc atcggcgtcg gcgatcttca
      241 gcgctgcgat catcaacccg agagaatcga ggccctgatc gggatcttcg ggccggtcgt
      301 tgacctgtcc gaccccgatg atgacgggaa tccgctcggg atcggtcatc gccacgatca
      361 gcgcgccttc cactcgggct tgcgcttctg cgcgaaggcg agcgggcctt cgcgcgcgtc
      421 ctcgctgcgc atcagtaccc cgcgttcgcg ggtgttatgc tcccaatagg gcgcgtcggc
      481 ggcgatgcgg ccttcgtcga tgccgagcgc gacgcgtttc gacgcctgca ccgacagcgg
      541 cgcattggcg gcgatcttct cggcgagtgc gatcgcggtg gggagcaggt cggcgagcgg
      601 gacgacatgg ttgacgagac cgaactcggc ggcgcgcgcg gcggggatcg gatcgccggt
      661 gagcatatat tccatcgcga gcttgcgggg cagctgctgc gcgaggcgga aggcgccgcc
      721 ggccgcggcg aacagcccga ccttgacctc gggcagcccg aattgcgcat gatcggcggc
      781 gataatgatg tcgctcatca gcgcgatctc gcagccaccg ccgaacgcga agccgttgac
      841 cgcggcgatg atcggtttcg agatcgggtg cgagaccatg ccggcaaagc cccacgcctg
      901 ctgcgccgga tcgtggggct ggaggctttc gccgcgcgac agcgcgacaa gatcggcgcc
      961 cgcgcagaag ctcttgtcgc ccgcgccggt gatcacgacg gcgcggattt cggggtcgtt
     1021 ctcggcttcc tcgagcgcgg tgccgatgcc gacatggacc gcggcattga ccgcgttgcg
     1081 cgcctcgggc cggttgatcg tgacgatcag gatgtggccg cggcattcgg tgaggatggt
     1141 ggtgtcggtc atcgcgaatc tctccctttc gggcgagtat cgcgggtggt tgacgtttac
     1201 gtcaactgct aaccgccacc ccagcgaaag ctggggtcgc tatcggccaa gcgccaagcc
     1261 gcaaccggcc ccagctttcg ctggggcgac gatgccttag atcgactggc cggtcttcgc
     1321 ccagtcggcg aggaagcctt cgatgccctt ttcggtcagg atatgtttga agaggccctt
     1381 gatcaccgag ggcggcgcgg tcatcacgtc ggcgccgatc ttcgcggctt cgagcacatg
     1441 gatgccgtgg cgcacgctcg cgacgaggat ttcggtctca tagccgtaat tgtcgtagat
     1501 cagccgaata tcggagatga gctgcatccc gtcgaagcca ttgtcgtcgt ggcggccgac
     1561 gaagggcgag acgaaggtcg cgccggcctt cgcggcgagc agcgcctgcg cggccgagaa
     1621 gcagagcgtg acattgacca tcgtgccgtc gccggtcagc gccttgcacg tcttgagccc
     1681 gtcgatcgtc agcggcacct tgatgcagac gttcggcgcg atcttgcgaa gaacctcggc
     1741 ctccttcatc atcgtggcat gatcgagcgc gacgacttcg gccgacacgg ggcccgtggt
     1801 cagcgcgcag atttccttcg tcacttcctt gaagtcgcgg cccgacttgg cgatcagcga
     1861 cgggttggtg gtgacgccgt cgagcaggcc cgtcgcggcg agctcctgga tgtcggcgat
     1921 ttcggcggtg tcggcgaaga atttcatggc acggggcttt ctggtgggaa ggtgattcgg
     1981 gtgttgctgc tcccctagag caacggcggc ggaaactgaa ccacatctgc ccacttcgtc
     2041 atgccggact tgatccggca tccatccgac gacggcggag tggaccccgg atcaagtccg
     2101 gggtgacgag gatttgaaac tgctagcgtt ccatatatgt tcccgctaag cgaaggtgca
     2161 tggccaaagc gaaacgtcaa tatgtctgcc agaattgcgg ctccgcgacc tatcgctggc
     2221 aggggcagtg cgccgactgc ggcgaatgga acacgctgat cgaggaggtg gctgaaacgg
     2281 tattctcggc gaagcacgac ctcagccgcg gcgggcggac gcttgcgctc gaaacgctcg
     2341 atgctgagag caagatgccc gaccgcatgc tctgcgggat cgccgaattc gatcgcgcat
     2401 tgggcggcgg gttcgttgcg gggtcggcga cgctgatcgg tggcgatccc gggatcggca
     2461 agtcgacctt gctgttacag gcggcgggga gactcgcgaa ggcgggcaag tcggtcgttt
     2521 atatcagcgg cgaggaggcc gcagcgcagg tgcggctgcg cgcgcagcgg ctggggctcg
     2581 gtgatgcgcc ggtcgcgctc gcgagcgcga cgtcggtgcg cgacatcctc gcgacgctcg
     2641 acaagcagac cgccgatttc gtggtgatcg attcgatcca gacgatgcac agcgacctga
     2701 tcgacagcgc gccggggacg gtgagccagg tgcgcgcaag tgcgcaggag ctgatccgct
     2761 atgccaagga cagcgatgcg gcgatcgtgc tcgtcggtca cgtcaccaag gacgggacga
     2821 tcgcggggcc gcgcgtgctc gaacatatgg tcgacacggt gctggcgttc gagggcgagc
     2881 gcagccacca atatcgcatc ctgcgcgcgg tgaagaaccg ctttggcggc accgacgaga
     2941 tcggggtgtt cgcgatgggc gaggaggggc tgggcgaggt gtcgaacccg tcgagcctgt
     3001 tcctgaccga ccgcagccgc gatgttccgg gatcggtggt gtttcccgcg ctcgagggca
     3061 cgcgcccggt gctcgtcgag gtgcaggcgc tgaccgttcg gctggcgagc ggcgcgactc
     3121 cccgtagagc ggtggtcggc tgggacagcg ggcggctcgc gatggtgctc gcggtgctcg
     3181 aagcgcgctg cgggctgcaa atgggcgccg ccgaggtcta tctcaacatc gcggggggct
     3241 atcggctcac cgatcccgcc gccgaccttg cggtcgccgc cgcgctgatc tcggcgttca
     3301 gcgagcgccc ggtgcccgcc gacgcgatcg tattcggcga gctgtcgctc tcgggcgagg
     3361 tgcggcaggt ttcgcatgat ggcttgcggc tgcgcgaagc ggcgaagctc ggtttctcgc
     3421 gcggttgggg tcccaagggc atgaaagcgg tcggcgggat ctcggtcacc ggtttcgaac
     3481 ggctcggcga actcgttgac ctgatgctcg ggcgcgacta gcgaccggcc tatgggaagt
     3541 ctgacggctc tggatatcgt cgtgctggtg ctggttggcg gcggcgcggt gctcggtttt
     3601 tcgcgcggcc tggtgcagga ggtgacgacg ctgctcgcgt ggattctcgc gatcgcggcg
     3661 gtgcgctttt ttcatccgct ggtgaccgat ctgctcgcag gctgggtcgg gaccgagggc
     3721 ggcgccgcgg cgctcgcctt cgtgctgctc ttcggcgggg tgttcgcgct cgccaaatgg
     3781 ggatcgcgtg cgatggggca gcgcagccgc gcgtcgcttg tcggcgggtt cgaccgcggg
     3841 ctcggtgcgg gtttcggcgc ggtgaagggc ctgttgatcg cgacgatcgg cttcatgatc
     3901 gtgacgctgc tctacgacat cggttatggt aacacgcagc gccccgtgtg gatgaccgac
     3961 agccgtacct atccagcgct caatgcaacg agcggcgcga tgagcaaggt gatcgccgag
     4021 cggcgcgccg aagcgcgcga ggccgaagcc gaggctgacg catcatgagc gcgccgctgt
     4081 acaatcgcga catattggcg ctcgcggtcg cgaccgccga ctatctgccg ctcgccgatg
     4141 cgcgccaccg tatcagcaag cgcgcgccgc tgtgcggcag cgcgatcatc ctcgatctcg
     4201 ataccgacga tgcgggccgc gtcacgcgcg tcggcatgca cgtcgaagct tgcgctctcg
     4261 gtcaggcgtc ggcggcgctg ctcgcgcgcc atgcgccggg actggggctc gccgacatc
//
LOCUS       DATMSU010000187         8815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_164214,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000187 DATMSU010000000
VERSION     DATMSU010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8815
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164214"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VN034_04805"
     CDS             complement(<1..272)
                     /locus_tag="VN034_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV59948.1"
                     /translation="MPLRDGPPSPDQLISDIAAARPVTIADYMAAANAHYYATRDPLG
                     AAGDFTTAPEISQMFGEMIGVWIADLWSRAGSPAFRYAELGPGRGTL"
     gene            complement(259..1077)
                     /gene="lgt"
                     /locus_tag="VN034_04810"
                     /pseudo
     CDS             complement(259..1077)
                     /gene="lgt"
                     /locus_tag="VN034_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
     gene            1212..2924
                     /locus_tag="VN034_04815"
     CDS             1212..2924
                     /locus_tag="VN034_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWV59949.1"
                     /translation="MPSALDLRLEAAYNLITGPGGPIQVGSVERFGQQLPVITNAPTN
                     LADYVAFFCAQHGDATFLVEGDERLSFKQVYAAARKVAAALVEGHGVERGDRVGIAMR
                     NANAWCVSYLGVLMAGGCATLLNGWWQGGELAAGIEDAEAKLVIADVQRAARLAEPGV
                     SHGAKVITVDLSKPIDEAIAPITSAGGSAGTALPALTGDDLATILFTSGSTGQSKGAY
                     SRHHAVVQAIFNYVTQTASIVHLLTQDGLMSDIQPATLICTPLFHVTAEIPVFLQSIA
                     IGRKLVLMPKWNADEAMRLIQDEKVNYFVGVPLMSYEILVSPNRKNYDLSTCKSYAGG
                     GAPRPPEHVRRLASEMGEAKPLLGYGLTETNAVGCGIINENYVDKPLSTGPASKPLVD
                     LAILDDNGEPVAQGGVGEVCIRSVCNFEGYWNNEAATKAAFFDNGYFRSGDLGYLDED
                     GYLFIVDRKKDIIIRGGENISCQEVEAAIYEHPEANECAVFGLPDERLGECVGAVVWM
                     NPGSSVTADDLAAFLGARLAPYKVPCKIWMSNDALPKLGSEKIDKVSLRSHYRGEYAA
                     EVVA"
     gene            2955..3797
                     /locus_tag="VN034_04820"
     CDS             2955..3797
                     /locus_tag="VN034_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWV59950.1"
                     /translation="MAEAETPVAPEKPEPVRVYRHRLPTRIWHWVNAVTLLVLLMSGL
                     MIFNAHPRLYWGEYGANFDRAWLVIGSTADAGYLRVGDWKIDTTGVLGRWTDAQGATK
                     TWAFPGWATIPTSYSLADGRRWHLLFAWMLSVGLALYMFWTLFGGHLLRDLHVRRAEW
                     SPRHIWRDIKDHARLRFPKGEAAARYGILQKLSYIGVIFIALPLMIATGLTMSPGMNA
                     AWPWLLDIFGGRQSARSIHFITAWALVAFFLVHIAMVLLAGPVNEIRSMVTGWFRLPR
                     ESET"
     gene            3794..4573
                     /locus_tag="VN034_04825"
     CDS             3794..4573
                     /locus_tag="VN034_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWV59951.1"
                     /translation="MSDIMLPRRQLIARAGGLVAAAGTLPLLAGCDAINDAPAVRKIL
                     SMGEEMNRASQRALIDRNALAREFSRADLSPYFRPNGTRLPAGPDYAAHAANGFVDWR
                     VKVSGLVARPLSLSMADIRAMPQRTQITRHDCVEGWSAIGQWTGVELHRILEAAGVRD
                     SARYIVFRCADRIGDALYYESCDMIDARHPQTILAWALNDAVLPIANGAPLRLRIERQ
                     LGYKHAKYVNAIEAVASLDQIGAGKGGYWEDRVDYEWYAGI"
     gene            4614..5864
                     /locus_tag="VN034_04830"
     CDS             4614..5864
                     /locus_tag="VN034_04830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HWV59952.1"
                     /translation="MSILRPFLSRIIRQGRLTVVTPDGAREEYGQPADGFPNVTIRFT
                     SRRGMRRIILDPRLGAAEAFMDGEMELVEGDIMELVGLIRGNTPWDRGARLHPKSWLG
                     RRAEWVKTRIGSINRQRRSRANVKHHYDIGNDLYRLFLDEDMQYSCAYWPRPDMTLEQ
                     AQAAKKAHIAAKLALAPGQRVLDIGCGWGGMAITLAKLEQVEVLGITLSEEQLALARQ
                     RAEAAGVADRVTFELIDYRDLAAREAGRFDRIVSIGMFEHVGAPNFEAFFRAAANLMT
                     ADGVMLLHTIGRFGKPGSTDAFTRKYIFPGGYIPALSETLAASEKSRLIATDIETLRL
                     HYALTLRQWYARALAHEAEIVRMMGVRFFRMWCFYLAGATSAFESGGMGNYQIQFARS
                     RHALPLTRDYMGEVENATSDSVRS"
     gene            complement(5839..6276)
                     /locus_tag="VN034_04835"
     CDS             complement(5839..6276)
                     /locus_tag="VN034_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWV59953.1"
                     /translation="MAKAEIKTKATEISVADFIAAVPDARRREEAAIVDAIHRRVTGQ
                     PPKMWGPSIIGYGSYDYVYDSGHSGTMCRAGFSPRKAAMTLYLMGHYCDRQPEADALL
                     ARLGKHKTGKSCLYLGKLADVDLAVLEQLVALSWDVMNERYPT"
     gene            6392..7609
                     /locus_tag="VN034_04840"
     CDS             6392..7609
                     /locus_tag="VN034_04840"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWV59954.1"
                     /translation="MSESFKRVVLAYSGGLDTSVILKWLQVTYGCEVVTFTADLGQGE
                     ELEPARAKAELMGIKPEHIYIDDLREEFVRDFVFPMMRANARYEGDYLLGTSIARPLI
                     SKRLVEIAKETGADAVAHGATGKGNDQVRFELSAYALNPDIKVIAPWREWDLTSRTAL
                     IDFAEKNQIPVPKDKRGESPFSTDANLLHTSSEGKVLEDPWEETPDYVYSRTVNPEDA
                     PDTPEYITIDFERGDGVALNGQGMSPATLLAALNDLGRKHGIGRLDLVESRFVGMKSR
                     GMYETPGGEIYARAHRGIESITLDRGAAHLKDELMPKYAELIYNGFWFAPEREMLQAA
                     IDHSQTNVTGTVRLKLYKGNASVVGRKSPFSLYSERHVTFEDDAGAYDQKDAAGFIKL
                     NALRLKLLAKQGQ"
     gene            7652..8131
                     /locus_tag="VN034_04845"
     CDS             7652..8131
                     /locus_tag="VN034_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59955.1"
                     /translation="MFSLLLAAASLTPAFNDVSYTDLGYVERTAGECRANFGGGSIEI
                     RQAFNDADRTMINVAVVTGKPTRALPVGTQLWAKASYGPAWRAVVIESTPERLGFVAH
                     NRFVGDLFTEDSLSIALHERLDAGAIGDAVLVDVGSDVSGFREKLASAFDCADALGI"
     gene            8193..>8815
                     /locus_tag="VN034_04850"
     CDS             8193..>8815
                     /locus_tag="VN034_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59956.1"
                     /translation="MRFFDFSSWQSIGATLLGLALVTLIGVGIRLMVMMTIQQRRERM
                     NRQINERLRTLMAAYKTLGGSFTGNLMVDPRHLRDIKRQAGEAKEEPAIDLSDEPAAE
                     GGGPGESDRPRRIRDAVEGALSDIILLGTEAQVRLASRAAQELAEGRPVHVHDLVVEL
                     RDFIRKALDLDPVPADVTIPVQGPTRPASSGSKGGARGGESRGGGGGR"
BASE COUNT         1589 a         2789 c         2916 g         1521 t
ORIGIN      
        1 agcgtgccgc gacccgggcc gagctcggca tagcgaaacg ccgggctgcc cgcgcgcgac
       61 cacaggtcgg cgatccagac gccgatcatc tcgccgaaca tctggctgat ctcgggcgcg
      121 gtggtgaagt cgccggcggc gccgagcggg tcgcgggtcg cgtaataatg cgcgtttgcg
      181 gccgccatat agtcggcgat cgtcaccggt cgcgccgccg cgatgtcgct tatgagctgg
      241 tcggggctgg gagggccgtc acgcaacggc atcgggtccg gcgatcggct cgatccgctg
      301 acggcgcccc ttcgcggtcg ccacgagcca gaaaccgatc agcagcatcg ggatggtgag
      361 cgtctgcccc atcgtcagcc cccacgacaa ggtgccgagc tggacgtcgg gttcgcgcac
      421 gaattcgacc gcgaaccggc tgaggccata gatgatcgcc gccatgccga acaggaaacc
      481 tggcttgtag cgcgcgtcgg tgcgccagaa gaaaaaggcg aggacggcca tcatcacgat
      541 cccttcgagg ccggcttcgt aaagctggct cgggtgacgc ggatccatcg tgccgctacc
      601 cggaaagatg atcgcccacg gcacggtcgt cgcgcggccc cacaattcgc cgttcacgaa
      661 attggcgagg cggccgaaga agaggccgaa ggggacgacg caggcgatat aatcgcaaaa
      721 gcgcagaaag ctgagtttct ctttcttcgt cacataccag atcgcgacca gcacgccgat
      781 gaccccgccg tggagcgaca tgccgccgtc ccacaatttg aagatttcga gcggcttcta
      841 gagataattg ccgaggttgt aaaagagcac atagccgagc cggccgccga ggatgatccc
      901 gagcatcgca tagaagatca tgtcgtcggc atggcgccgc gccatcggcg cgccgggctg
      961 cgcgatcagc ttcaacagat accagtaacc gacgaaaatg ccggcgagat aggcgagcgc
     1021 ataccagcgg atggggaggc cgaagacgct gaacgcgatc tcgctgttcg gtcccatcag
     1081 ctggtcgaag ttcacatatt gcgttgcttc ggctaaggtt gcaaaaagaa acatattgtc
     1141 gcgctgtcct tccccaggag aggcctcccc ataaggggta aggcgagcga gaccaagagg
     1201 agagatagaa gatgccatca gcgctcgatt tgcgcctgga agccgcttac aatctgatca
     1261 ccggcccggg cgggccgatc caggtcgggt ccgtcgaacg ctttggccag caactgcccg
     1321 tcatcaccaa tgcgccgacg aatctggccg actatgtcgc ctttttttgt gcgcagcatg
     1381 gcgacgcgac ctttctggtc gagggcgacg agcggctgag cttcaaacag gtctatgccg
     1441 ccgcgcgcaa ggtcgccgcg gcgctcgtcg aagggcatgg cgtcgagcgg ggcgaccgcg
     1501 tcggtatcgc gatgcgcaac gccaatgcct ggtgcgtctc ctacctcggc gtgctgatgg
     1561 cgggcggctg cgcgaccctt ctcaacggct ggtggcaggg cggcgaactc gccgcgggga
     1621 tcgaagatgc tgaagcaaag ctggtcatcg ccgacgtcca gcgcgcggcg cggctcgcgg
     1681 agccgggcgt cagccatggc gcaaaggtca tcacggtcga tctttcaaag ccgatcgacg
     1741 aagcgatcgc cccgatcacg agcgcaggcg gcagcgccgg gacggccttg cccgctctca
     1801 ccggcgacga tcttgcgacg atcctcttca cctcgggctc gaccggccag tcgaagggcg
     1861 catattcgcg ccaccatgcg gtggtgcagg cgatcttcaa ctatgtcacg caaaccgcga
     1921 gcatcgtcca tctgctcacg caggacgggc tgatgtcgga catccagccc gcgacgctga
     1981 tctgcacccc gttgttccac gtcaccgcgg aaattccggt gttcctccag agcatcgcga
     2041 tcgggcgcaa gctggtcctg atgcccaagt ggaatgcgga cgaggcgatg cggctcattc
     2101 aggacgagaa ggtcaattat ttcgtcggcg ttccgctgat gagctatgaa attctcgtca
     2161 gcccgaaccg caagaattac gatttgtcga cgtgcaagag ctacgccggc ggcggcgcgc
     2221 cgcgtccgcc cgaacatgtg cggcgccttg cgtcggagat gggtgaagcg aagccgttgc
     2281 tcggttacgg cctgaccgaa accaatgcgg tcggctgcgg catcatcaac gagaattatg
     2341 tcgacaagcc gctgtcgacg ggaccggcgt cgaagccgct cgtcgatctc gcgatcctcg
     2401 acgacaatgg cgagccggta gcgcagggcg gcgtcggcga agtgtgcatc cgatcggtct
     2461 gcaatttcga gggctattgg aacaatgagg cggcgacgaa ggcggccttc ttcgacaatg
     2521 gctatttccg ctcgggcgac ctcggctatc tcgacgagga cggctatctg ttcatcgtcg
     2581 accgcaagaa ggacatcatc atccgcggcg gcgagaatat cagctgtcag gaggtcgagg
     2641 cggcgatcta cgagcatccc gaggcgaatg aatgcgcggt gttcggcctg cccgacgagc
     2701 gactcggcga atgcgtcggc gcggtggtgt ggatgaaccc gggcagcagc gtgaccgccg
     2761 acgatctggc cgccttcctc ggcgcgcgcc tcgcgcctta caaggtgccg tgcaagatat
     2821 ggatgtcgaa cgacgcgctg cccaagcttg gcagcgaaaa gatcgacaag gtcagcttgc
     2881 ggagccacta tcgcggcgaa tatgcggcgg aggtcgttgc gtaaccgatt gaaggatagg
     2941 agcgaaccgg gatcatggcc gaggctgaaa ctcccgtcgc tcccgaaaag cccgagccgg
     3001 tgcgcgtcta tcgccaccgg ctgccgacgc gcatctggca ttgggtcaat gcggtcacgc
     3061 tgctcgtcct cctgatgagc gggctgatga tcttcaatgc gcacccgcgc ctctattggg
     3121 gcgaatatgg cgccaatttc gatcgcgcgt ggctggtcat cggctcgacc gccgacgcgg
     3181 gctatttgcg cgtcggcgac tggaagatcg acacgacggg cgtgctcggg cggtggaccg
     3241 acgcgcaagg ggcgacaaag acctgggcct ttccgggctg ggcgacgatc ccgacgagct
     3301 acagccttgc cgacgggcgg cgctggcatc tgcttttcgc gtggatgctg tcggtcgggc
     3361 tggcgctcta tatgttctgg acgctgttcg gcgggcattt gctgagggat ctgcacgttc
     3421 gccgcgccga atggtcgccg cgccacatct ggcgcgatat caaggaccat gcgcggctgc
     3481 gttttccgaa gggcgaagcc gccgcgcgct acggcatcct gcaaaaattg agctatatcg
     3541 gcgtcatctt catcgcgctg ccgctgatga tcgcgacggg gctcactatg tcgcccggca
     3601 tgaacgccgc ttggccgtgg ctgctcgaca ttttcggtgg gcgccagtcg gcgcgctcga
     3661 tccacttcat caccgcctgg gcactcgtgg cctttttcct cgtccatatc gcgatggtgc
     3721 tgcttgcggg gccggtgaac gagataaggt cgatggtcac cggctggttc cgcttgccgc
     3781 gggagagcga gacgtgagcg acatcatgct tccgcgccgc caattgatcg cgcgcgccgg
     3841 cgggttggtc gccgcagcgg ggacgctccc gctgctcgcg ggctgcgacg cgatcaacga
     3901 cgcgcccgcg gtgcgcaaga tcttgtcgat gggcgaggag atgaaccgcg caagccagcg
     3961 cgcgctgatc gaccgcaatg cactggcgcg cgaattcagc cgcgccgacc tgtcgcccta
     4021 tttccggccg aacggcacgc gcctccccgc cgggcccgat tatgccgcgc atgccgcgaa
     4081 cggttttgtg gactggcgcg tcaaggtctc gggcctcgtc gcgcgcccgc tgtcgctgtc
     4141 gatggccgac atccgcgcga tgccgcagcg cacgcagatc acgcgccacg actgcgtcga
     4201 ggggtggagc gcgatcgggc aatggaccgg cgttgagctt caccggattc tcgaagcggc
     4261 gggggtgcgg gacagtgcgc gctatatcgt cttccgctgc gccgaccgga tcggcgacgc
     4321 gctctattat gaaagctgcg acatgatcga cgcgcgccat ccgcagacga tcctcgcctg
     4381 ggcgctgaac gatgccgtgt tgccgatcgc caacggcgcg ccgctgcgcc tccgcatcga
     4441 gcgccagctg ggctacaagc acgccaaata tgtcaacgcg atcgaagcgg tggcgagtct
     4501 cgaccagatc ggcgcgggca agggcggcta ttgggaggat cgcgtggatt atgaatggta
     4561 tgccggaatt tgacctcggc cccgtcgcgg tgcaaatatt cggcgcataa catatgtcga
     4621 ttctgcgtcc gtttctctcc cggatcattc gtcaggggcg gctgaccgtc gtcacgcccg
     4681 atggcgcgcg cgaggaatat ggccagccgg ccgacggctt tcccaatgtc acgatccgct
     4741 tcaccagccg caggggaatg cgccgcatca tcctcgaccc gcgtctcggc gcggccgaag
     4801 cgttcatgga tggcgagatg gagctggtcg aaggcgacat catggagctc gtcggcctga
     4861 tccgcgggaa cacgccgtgg gatcgcggcg cgaggctcca ccccaagagc tggctcggcc
     4921 gccgcgccga atgggtgaag acgcggatcg gatcgatcaa ccgccagcgc cgctcgcgtg
     4981 ccaacgtcaa gcatcattac gacatcggta acgacctcta ccgcctcttc ctcgacgagg
     5041 acatgcaata tagctgcgcc tattggccgc ggccggacat gacgctggaa caggcgcagg
     5101 ccgcgaaaaa ggcgcatatc gccgccaagc tcgcgctcgc gcccggacag cgggtgctcg
     5161 acatcggctg cggctggggc gggatggcga tcacgctcgc gaagctcgaa caggtcgagg
     5221 tgctcggcat cactctgtcc gaggagcaac tcgcgctcgc gcgccagcgc gctgaggcgg
     5281 cgggggtcgc cgaccgggtg acattcgagc tgatcgacta tcgcgacctc gccgcgcgcg
     5341 aggcgggacg gttcgaccgc atcgtgtcga tcggcatgtt cgagcatgtc ggcgcgccca
     5401 atttcgaggc cttctttcgc gccgccgcca atctgatgac cgccgacggg gtgatgctgc
     5461 tgcacacgat cggtcgcttc gggaagccgg gatcgaccga cgctttcacg cgcaaatata
     5521 tctttcccgg cggctatatc ccggcgctca gcgagacgct ggcggcgagc gaaaagagcc
     5581 ggctgatcgc caccgacatc gaaacgctgc ggctgcatta tgcgctgacc ttgcgccaat
     5641 ggtatgcgcg ggcgctggcg catgaggcgg agatcgtccg gatgatgggc gttcgcttct
     5701 ttcgcatgtg gtgcttctat ctcgccggcg cgacatcggc gttcgagagc ggggggatgg
     5761 gcaattacca gatccagttc gcgcgcagcc gccacgcgct gccgctgacc cgcgattata
     5821 tgggagaggt ggagaacgct acgtcggata gcgttcgttc atgacatccc agctcagcgc
     5881 cacgagctgt tcgagaaccg ccaaatccac gtcggcgagc ttgcccaaat aaaggcagga
     5941 tttgcccgtc ttgtgctttc cgagcctggc gagcagcgcg tcggcctcgg gctggcgatc
     6001 gcaataatgg cccattagat agagcgtcat cgcggccttg cggggtgaga aaccggcgcg
     6061 gcacatcgtt cccgaatggc cgctgtcata gacataatca tagctgccat agccgatgat
     6121 cgacggaccc cacatcttgg gcggctggcc cgtgacgcgg cggtggatcg catcgactat
     6181 cgccgcttcc tcgcggcgcc tcgcgtcggg gaccgcggcg atgaaatcgg cgaccgaaat
     6241 ctcggtcgcc ttggtcttga tttcggcttt ggccatggcg tttctcccaa gcgccgacga
     6301 tagcctgcgc gcggcggggc gccaagccgg ttgcgcgttc gccggctccc tgctagccgc
     6361 gcccgcgtct ttccctcgcc aaaggtgcat catgtccgag tccttcaagc gcgtcgtcct
     6421 cgcctattcg ggcggcctcg ataccagcgt catcctgaag tggctgcagg tgacctacgg
     6481 ctgcgaggtc gtgaccttca cggccgacct cgggcagggc gaggaactcg agcccgcgcg
     6541 cgccaaggcc gagctgatgg ggatcaagcc cgagcacatt tatatcgacg acctgcgcga
     6601 ggaattcgtg cgcgatttcg tctttccgat gatgcgcgcc aacgcgcgct acgagggcga
     6661 ttatctgctc ggtacctcga tcgcgcgccc gctgatctcg aagcggctcg tcgagatcgc
     6721 gaaggaaacc ggcgccgacg cggtcgcgca cggcgcgacg ggcaagggca atgaccaggt
     6781 gcgcttcgag ctgtcggcct atgcgctcaa ccccgacatc aaggtgatcg cgccgtggcg
     6841 cgaatgggac ctgaccagcc gcaccgcgct gatcgacttc gccgagaaga accagatccc
     6901 ggtgccgaag gacaagcgcg gcgaaagccc cttctcgacc gacgcgaacc tcctccacac
     6961 ctcgtcggag ggcaaggtgc tcgaggatcc gtgggaagag accccggact atgtctattc
     7021 gcgtaccgtg aatcccgagg atgcgcccga cacgcccgaa tatatcacga tcgatttcga
     7081 gcgcggcgac ggtgttgcgc tgaacggtca ggggatgtcg cccgcgacgc tgctcgcggc
     7141 cttgaacgac ctcggccgca aacatggcat cggccgcctc gatctggtcg agagccgctt
     7201 cgtcggcatg aagtcgcgcg gcatgtacga gacgcccggc ggcgaaatct atgcgcgcgc
     7261 gcatcgcggc atcgaaagca tcacgctcga ccgcggcgcg gcgcatctca aggacgagct
     7321 gatgccgaaa tatgccgagc tcatctataa cggcttctgg ttcgcccccg agcgcgagat
     7381 gctgcaggcc gcgatcgacc atagccagac gaacgtcacc ggcaccgtcc ggctcaagct
     7441 ctacaagggc aatgccagcg tcgtcggccg caagtcgccg ttcagcctct acagtgaacg
     7501 ccatgtgacc ttcgaggacg atgccggcgc ctatgatcag aaggatgcgg cgggctttat
     7561 caagctcaac gccttgcgct tgaaactgct cgcgaaacag ggtcaataaa gcggccggga
     7621 acgggcccgg ctgcgacagg agccttggtc gatgttctcc cttttgctgg cggccgccag
     7681 tctgacaccc gcattcaacg acgtgtccta taccgacctc ggctatgtgg agcgcaccgc
     7741 gggcgagtgc agagcgaatt tcggcggcgg gtcgatcgag atccgtcagg ccttcaacga
     7801 tgccgatcgc acgatgatca acgtcgcggt ggtgaccggc aagccgacac gcgcgctgcc
     7861 cgtcggcacg cagctgtggg cgaaggcaag ctatggtccc gcgtggcgcg cggtggtgat
     7921 cgaatcgaca ccggagcggc tgggttttgt ggcgcataat cgcttcgtcg gcgacctgtt
     7981 caccgaggat tcgctgtcca tcgcgctcca cgagcggctc gatgcgggcg cgatcggcga
     8041 tgccgtgctc gtcgatgtgg gatcggacgt ttcgggcttt cgcgaaaagc tcgcttcggc
     8101 cttcgactgc gccgacgctc tcggcatctg atccggaaaa agcaccgatt ttcgggtagc
     8161 gccgcgccgc gaagggcggc actatcgccg ccatgcggtt cttcgatttt tcgtcgtggc
     8221 aaagcatcgg ggcgacgctg ctcggcctcg cgctcgtcac gctgatcggc gtcggtatca
     8281 ggctcatggt catgatgacg atccagcagc gccgcgaacg gatgaaccgc cagatcaacg
     8341 agcggctgcg taccctgatg gcggcgtaca agacgctcgg cggctccttc accggaaatc
     8401 tgatggtcga tccgcgccac ctgcgcgata tcaaacgcca ggcgggcgag gccaaggagg
     8461 agcccgcgat cgacctgtcc gacgagccgg cggccgaagg gggtgggccc ggcgaatcgg
     8521 accggccgcg ccgtattcgc gacgcggtcg aaggcgcgct gtccgatatc atcctgctgg
     8581 gcaccgaagc gcaggtccgc cttgcgagcc gcgcggcgca ggaactagcc gaggggcggc
     8641 cggtgcatgt ccacgacctt gtcgtcgaac tgcgcgattt catccgcaag gcgctcgacc
     8701 tcgatccggt tcccgccgat gtcaccattc cggtccaggg accgacacgg ccggcgagca
     8761 gcgggagcaa gggcggcgct cgcggcggcg agtcgcgcgg cggtggaggg ggacg
//
LOCUS       DATMSU010000188         1593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_3673, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000188 DATMSU010000000
VERSION     DATMSU010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3673"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VN034_04855"
     CDS             complement(<1..812)
                     /locus_tag="VN034_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV59957.1"
                     /translation="MPSEHDNDCLQVRREGGVLWLTMNRPAALNAIDRILGLELLKAF
                     HAAERDDDVRAVVLTGSGRAFCAGDDIAGLKAFLEGDFDHERAAVDPTDKSSLYMRVA
                     QLMTRCPKPVIAAVNGLSFGAGTELACAADIRCMARSAAMGSGLVKIAQLGNAAYLGH
                     VVGPARAFEIFATGRAVGAEEAMAIGLATHMFDDGEFMDGVAALADRLANGPTRIIAM
                     QKRLLAASEGRNLDARAMLQEAAHQTCFFETEDAREGASAFIENRKPTFTGR"
     gene            complement(796..>1593)
                     /locus_tag="VN034_04860"
     CDS             complement(796..>1593)
                     /locus_tag="VN034_04860"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV59958.1"
                     /translation="VSGAAGAMGVATARLLRAAGARLCLTDRHVDPIELLAKELDRTG
                     QEVIVIACDQAEAEDVDRLSAQLSDRLGALDGAFVNAGIGRYGALLDVAVKDWKQHVD
                     VNLTGSFLVAQAAARLMIDTRRGGALVFNASTAAAHAAELFGAYGASKAGLRMLSRTL
                     AGELGAHGIRANLIMPGVIETRMTSGLLADPDISADIVGETPIGRLGQAEDIAELALF
                     LLSDRSTFITVAEILIDGGQTLHGYPRWFSRTPGGANHDWSLHAKRT"
BASE COUNT          285 a          540 c          499 g          269 t
ORIGIN      
        1 cacctgcccg taaatgtcgg cttgcggttc tcgataaagg cgctggcacc ttccctcgca
       61 tcttcggttt cgaaaaaaca ggtctggtgc gcggcttcct gcaacatggc gcgggcgtcg
      121 agattgcgtc cctccgacgc cgcgagcagc cttttctgca tcgcgatgat gcgggtcgga
      181 ccgttcgcca gccggtccgc caaagccgcg acgccatcca tgaattcgcc gtcgtcgaac
      241 atatgggtcg cgaggccgat cgccatcgcc tcctccgctc cgacggcacg cccggtcgcg
      301 aaaatctcga acgcgcgcgc gggtccgacg acgtggccga gataggcggc attcccgagc
      361 tgtgcaattt tgacgagccc gcttcccatg gcggcagagc gcgccatgca ccttatgtcc
      421 gcagcacacg caagttcggt gccggcgccg aacgacaggc cgttgaccgc cgcgatcacg
      481 ggcttgggac accgggtcat gagctgtgcg acccgcatgt aaaggctgga cttgtcggtc
      541 ggatcgacag cagcccgctc atgatcgaag tcgccttcga ggaaagcctt gagacccgcg
      601 atatcgtcac cggcgcaaaa agcgcggccc gaaccggtaa ggacgaccgc ccgaacatca
      661 tcgtcgcgtt ccgccgcgtg aaaggccttg aggagttcaa gacccaatat ccggtcgatc
      721 gcgttcagcg ctgccggccg gttcattgtc agccagagaa cgccgccctc gcggcgcacc
      781 tgcaggcagt cgttgtcatg ttcgcttggc atggaggctc cagtcatgat ttgcgccgcc
      841 cggcgtgcgg ctgaaccaac gcggatagcc gtgcagcgtc tggccgccgt cgatcaggat
      901 ttcggccacg gtgatgaagg tggaacggtc gctcaacagg aaaagcgcaa gctcggcgat
      961 gtcttccgct tggcccaagc ggccgatcgg tgtctcgccg acgatgtcgg cgctgatgtc
     1021 cggatcggcg agaagccccg acgtcatccg cgtttcgatg accccgggca tgatcaggtt
     1081 ggcacgaatg ccgtgcgcgc cgagttcacc cgcgagcgtc cgcgaaagca tccgcagacc
     1141 cgccttcgat gcgccataag cgccaaacag ctctgccgca tgggcggcgg cggtcgacgc
     1201 gttgaacacc aaggctccgc cccggcgcgt gtcgatcatc agccgggcag cggcctgagc
     1261 gacgagaaag cttcccgtca ggttgacgtc gacatgctgc ttccagtcct tgaccgccac
     1321 gtccagcagc gcgccatagc gaccgatacc ggcgttgacg aaggcaccgt cgagcgcgcc
     1381 gagacgatcc gaaagctgtg cggaaagacg atcgacatcc tctgcctcgg cttgatcgca
     1441 ggcgatgacg atcacctcct gtcccgttcg gtccagttcc ttcgcgagca gttcgatcgg
     1501 gtcgacatgc cggtcggtca ggcacaggcg ggctccggcc gcccgcagca gccgcgcggt
     1561 cgcgaccccc atggcgcccg ccgcgcccga cac
//
LOCUS       DATMSU010000189         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_164225,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000189 DATMSU010000000
VERSION     DATMSU010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164225"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VN034_04865"
     CDS             complement(<1..273)
                     /locus_tag="VN034_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV59959.1"
                     /translation="MRKLLFLLSLSTAGCAVGPHYEPPQPVVKSAGAYTSAAPGVDSA
                     VALPDHWWRLYDDPVLDGLIERALAANTDLRVASANLAKAQAVLGET"
     gene            complement(266..3343)
                     /locus_tag="VN034_04870"
     CDS             complement(266..3343)
                     /locus_tag="VN034_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV59960.1"
                     /translation="MAFTDIFIRRPILAVVVSLLILLVGGASLFSLPVREYPNMESAT
                     IVVDTGYPGATQDVMQGFVTTPIAQAIATANGIDYLTSTSTLGKSQVKAKLVLNADAD
                     RAMTEILAKVQQVKYRLPAGANDPVITKMTDGASAVQYLAFVSDSLPVPQITDFASRI
                     AQPLITAVPGVASAEISGGQTLAMRIWIDPVRLAARRLSASDIAAALRANNVQAAPGR
                     LKGADTAINITAGTDLRDLDAFRQMVVKRVDGGIVKLGDVATVEIGGQNYDSSSMTSG
                     RRSVTIAITPTPDGNPLEIVKAVKALLPGIQRVAPPSVEVISQFDVAHFVNASIDEVS
                     HTLVEAIVIVIVVIFLFLGSLRAVLIPVVTIPLSLVGTAALMLAFGFSLNLLTLLAMV
                     LAIGLVVDDAIVVVENIHRHIEEGLSPVRAALLGAREIVGPVVGMTLTLAAVYAPIGL
                     MGGLTGALFREFAFTLAGAVVVSGVVALTLSPMMSSLLLNSQMTEGKLSRAIERNMQF
                     ATASYGRLLDATLAARPAVLLVGAVVLGAIVILFTGAQRELAPQEDQGYVFVQTKAPQ
                     YANVDYTARYAHEVEQLFRGLPEYVSSWFDNGTDGLNNGFGGVILTEWDERERSADAI
                     QAELNAKGAGITGVYAASFQAPALPASSGGLPVQMVIRSSDDFKTIHAALEKVKGAAW
                     QSGLFVFVDSDLAFDSPEARIGIDRAKAGEMGVTMEAIADTLATMVGENYVNRFNWHD
                     RSYDVIAQVPAAQRLTPDNLGQYHVKTASGALVPLSTVTKVEMRPEANRLTQFNQMNS
                     ATLSAVLAPGVTMGQAVDFLKSQPLPAGTSVDWLSDSRQYVTEGDRLTISFGFALIVI
                     FLVLAAQFESFRDPLVILVTVPLAICGALVPLWLGYTTLNIYTQIGLVTLIGLISKHG
                     ILMVSFANQIQKQDGLDRVAAIRKSAAVRMRPVLMTTAAMVAGLVPLLFANGAGASSR
                     FAIGIVVVMGMLVGTLFTLFVLPTIYSLIARDHRAASHSARETELAQAEVAYA"
BASE COUNT          621 a         1085 c         1113 g          583 t
ORIGIN      
        1 tgtttcaccc agcacggcct gcgctttcgc aagattggcg ctcgccacgc gcaggtcggt
       61 gttcgcggcg agcgcgcgct cgatcaggcc atcgagtacc ggatcgtcgt aaagccgcca
      121 ccaatggtcg ggcaacgcga ccgcgctgtc gacgcccggc gccgcgcttg tgtacgcacc
      181 cgccgatttc accaccggct gcggcggctc ataatgcggg ccgacggcgc agccggcggt
      241 ggacagggac agcaggaaaa gcagcttacg catatgcgac ctccgcctgc gcgagttcgg
      301 tttcgcgcgc gctgtgcgac gccgcgcgat gatccctggc gatcaggctg tagatggtgg
      361 gaaggacgaa gagggtgaac aaggtaccga ccagcattcc catcacgacg acgataccga
      421 tggcgaaacg gctggaagcg ccggcgccat tggcaaagag caacgggacg agcccggcga
      481 ccatcgccgc cgtggtcatc agcaccggac gcatccgcac cgccgccgat ttgcggatcg
      541 ccgcgacccg gtcgagccca tcctgtttct ggatctggtt ggcaaaggac accatcagga
      601 tgccgtgctt ggaaatgagg ccgatcagcg tcaccagccc gatctgggta tagatgttga
      661 gcgtcgtgta gccgagccac agcggcacca gcgcgccgca tatcgccagc ggcaccgtaa
      721 ccaggatcac cagcggatcg cggaagcttt cgaactgggc cgcgagtacc agaaatatga
      781 cgatcagcgc aaagccgaac gatatcgtca ggcggtcgcc ttcggtcaca tattggcggc
      841 tgtccgaaag ccagtcgacg ctggttccgg cgggcagggg ctggctcttc aggaaatcga
      901 ccgcctgtcc catcgtgacg ccgggggcga ggacggccga aagcgtcgcc gagttcatct
      961 ggttgaactg tgtcaggcgg ttcgcttcgg gacgcatctc caccttggtc accgtcgaca
     1021 gcgggaccag cgcgcccgac gccgtcttga catgatattg gccgagattg tcgggcgtca
     1081 gccgctgtgc cgccggcacc tgcgcgatga cgtcgtagga ccggtcgtgc cagttgaagc
     1141 gattgacgta attttcgccg accatcgtcg ccagcgtgtc ggcgatggct tccatcgtga
     1201 cccccatttc gcccgctttc gcgcggtcga tgccgatccg tgcttcgggg ctgtcgaacg
     1261 caaggtcgct gtcgacgaaa acgaacaggc cgctctgcca ggccgcgcct ttcacctttt
     1321 ccagtgcagc atggattgtc ttgaaatcgt ccgacgaacg gatgaccatc tgcaccggca
     1381 agccgccgct cgatgccggt agggcgggag cctggaagga agcggcatag acgcccgtta
     1441 ttcccgcgcc cttggcgttg agttcggcct ggatggcgtc ggcactgcgt tcgcgttcgt
     1501 cccattcggt caggataacg ccgccaaatc cgttgttgag cccgtcggtg ccattgtcga
     1561 accagctgct cacatattcg ggaagccccc ggaacaattg ctcgacctcg tgcgcatagc
     1621 gcgcggtata gtcgacattg gcatattgcg gcgccttggt ctggacgaag acatagccct
     1681 gatcttcctg cggcgcgagc tcgcgctggg cgccggtgaa caggatgacg atcgcgccca
     1741 gaacgacggc gccgaccagc agcaccgcgg gtcgcgccgc cagtgtggcg tcgagcaggc
     1801 ggccatagct tgccgtcgcg aactgcatat tccgttcgat cgcgcgcgac agcttgccct
     1861 cggtcatctg gctgttgagc agcaggctgc tcatcatcgg cgacagggtc aaggcgacaa
     1921 cccccgacac cacgaccgct ccggcgaggg tgaaggcgaa ttcgcggaac aatgcgcccg
     1981 tcagcccgcc catcaagccg atcggcgcgt agaccgccgc cagcgtcaac gtcatgccga
     2041 ccaccggccc gacgatttcg cgcgcgccga gcagcgcggc gcgaaccggc gacaggccct
     2101 cttcgatatg gcggtggata ttctcgacca ccacgatcgc gtcgtcgacg accagcccga
     2161 tcgccagcac catggcgagc agggtcaaaa gattgagcga aaatccgaaa gccagcatca
     2221 acgcggcggt tccgaccagc gacagcggaa ttgtcaccac ggggatgagc accgcgcgca
     2281 gcgaaccgag gaacaggaag atcacgacga tcacgatcac gatcgcctcg accagcgtat
     2341 ggctgacctc gtcgatcgag gcgttgacga aatgagcgac gtcgaactgg ctaatgacct
     2401 cgacgctcgg cggcgccacc cgctggatcc cgggaagcag cgccttgacg gctttcacga
     2461 tctccagcgg attgccgtcg ggcgtcgggg tgatggcgat ggtcaccgat cgccggcccg
     2521 aggtcatcga gctgctatcg taattttgcc cgccgatttc gacggtggcg acgtcgccca
     2581 gcttcacgat gccgccatcg acgcgcttca ccaccatctg gcgaaacgca tcgaggtcgc
     2641 gcaggtcggt tccggcggtg atgttgatcg cggtgtcggc gcctttcagg cggcccggtg
     2701 cggcctggac gttgttggcg cgcaaggcgg cggcgatatc gctcgccgac agcctccgcg
     2761 cggcgagacg cacggggtcg atccatatcc gcatcgccag cgtctgcccg ccgctgattt
     2821 ccgccgatgc gacccccgga acggcggtaa tcagcggttg cgcgatacgc gaagcaaaat
     2881 cggtgatctg gggtacgggc aggctgtcgc tgacgaatgc gagatattgt accgccgacg
     2941 cgccgtcggt catcttggtg atgacgggat cgttggcgcc ggcgggcagg cgatatttca
     3001 cctgctgcac cttcgccaat atctcggtca tcgcgcggtc ggcgtcggca ttcagcacca
     3061 gcttggcctt gacctggctc ttgccgaggg tggatgtcga ggtgagatag tcgatgccgt
     3121 tggcggtcgc gatcgcctgc gcgatgggcg tggtgacgaa tccctgcatc acgtcctgcg
     3181 tggcgccggg atagccagtg tcgacgacga tggttgcgct ttccatgttc ggatattcgc
     3241 ggaccggcag cgaaaagagc gatgcgccgc cgacgagcag gatcagcaag ctgacgacca
     3301 ccgcgaggat cggacggcgg atgaagatgt cggtaaaggc catctcagcg cgctcccttg
     3361 cgcggcgccg gacgggcggg cgccagcgtg accggcgcgc cg
//
LOCUS       DATMSU010000190         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145335,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000190 DATMSU010000000
VERSION     DATMSU010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145335"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VN034_04875"
     CDS             <1..478
                     /locus_tag="VN034_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HWV59961.1"
                     /translation="WTTSLTAATLPENQPYPALYSALGAVLDAIGVAASARQWALALG
                     RYELLLLAELGFGLDLGECVVTGSTGDIAFVSPKSGGAVSAAAAAGYEKRLLRLPPFM
                     RHDDPDKAASDPTMADILDFLAITGHFLARDVLDGRNRDLLGVRERLVSRLGRAVA"
     gene            524..1573
                     /gene="leuB"
                     /locus_tag="VN034_04880"
     CDS             524..1573
                     /gene="leuB"
                     /locus_tag="VN034_04880"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWV59962.1"
                     /translation="MLKILLLAGDGIGPEIMVEAEKVLGALDLPVTLDRALVGGAAYE
                     ATGHPLPPETLAKAKAADALLFGAVGDPRFDNVERHLRPEQAILGLRAELGLFANLRP
                     AKLFAGLEDSSALRPEVASAIDLLIVRELNGDVYFGEKGMRTTPSGEREGYDVMSYSE
                     SEVRRIAHVAFRAAQGRAKRLCSVDKANVLETSQLWRDVVIGVAADYPDVALTHMYVD
                     NAAMQLVRNPGQFDVVVTGNLFGDILSDQASMCVGSIGLLASASLSEGKLGLYEPIHG
                     SAPDIAGKGVANPLAMILSLAMLLRHSGGDEASAARIEAAVAKVLADGARGADLGGNM
                     GTAALGDAVVSVLNG"
     gene            1579..2787
                     /locus_tag="VN034_04885"
     CDS             1579..2787
                     /locus_tag="VN034_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWV59963.1"
                     /translation="MKKTTGFDRSKTRSWHPATQAVRGGTWRSEHGETSEALFLTSGY
                     TYESAEEVAARFAGEEQGMTYSRLQNPTVAMLEERIALMEGAEACRVQATGMAAMTTA
                     LLCQLSAGDHIVAAKAAFGSCRWLVDHLCPRFGIETTVVDGRDNDAWEKAIKPNTKVF
                     FFETPANPTMDIVDMKHVCALAKAHGITTVVDNAFATSVLQRPMDFGADVVAYSATKL
                     MEGQGRVLAGAVCGTGKFINDVLLPFQRNTGPNLSPFNAWVVLKGLETLDLRARRQSE
                     NALAVGKAIEGRVARMLHPGLASHPQHNLAMSQSSACGPIFSFVLDGGREQAMGFLNA
                     LELVDISNNIGDSRSLCCHPWSTTHYGVSEEARIDMGVVEGMLRINIGLEDPRDVIAD
                     LDQALTKVGL"
     gene            2805..3206
                     /gene="apaG"
                     /locus_tag="VN034_04890"
     CDS             2805..3206
                     /gene="apaG"
                     /locus_tag="VN034_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWV59964.1"
                     /translation="MIDSFFPFAATTGSITVRVAVSYLPEQSHPALGRWFWAYHVRVE
                     NHGDDAVQLISRHWRISDGRGQTSEVEGEGVVGEQPLIMPGGAYDYVSGCPLPTPEGS
                     MVGSYAMELGDGSQMLVAIPHFPLVAPATAS"
     gene            3203..>3782
                     /locus_tag="VN034_04895"
     CDS             3203..>3782
                     /locus_tag="VN034_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV59965.1"
                     /translation="MKRTHLPLNALRVFDAAARHLSFTRAADELAVTPAAVGQQIRAL
                     EDMLGVVLFRRTPKGLELTGEANAGLDALRQGFLQFEESVRAMQAGQSSQSLTIAAPR
                     DLTAKWLAPRLARYSQQAPDVRFTLVSADSGIDFTEANLDLAIFWTDGAGEHEGVALA
                     EAMMVTVAGPASESETRIAWPGCPVEDGEPALR"
BASE COUNT          633 a         1215 c         1289 g          645 t
ORIGIN      
        1 ctggacgacg agcctcaccg cggcgacgct gcccgaaaac cagccatatc cggcgctcta
       61 ttcggcgctg ggcgcggtgc tcgacgccat cggcgtcgcg gcgtccgcgc gccaatgggc
      121 gctggcgctc ggccgttacg aactgcttct ccttgccgag ctgggatttg gactcgatct
      181 cggcgaatgc gtcgtcacgg gatcaaccgg cgatatcgct tttgtcagcc ccaaatcggg
      241 cggcgcggtt agcgcggccg ccgccgccgg atatgaaaaa cgcctgcttc gccttccccc
      301 gttcatgcgg cacgacgatc ccgataaagc agcttccgat ccgacgatgg ccgacatact
      361 cgacttcctc gcgatcaccg gccatttcct cgcccgcgac gtcctcgacg ggcgcaaccg
      421 cgacttgctg ggcgtaaggg aaagattggt ctcccggctg ggcagggcgg ttgcgtgacg
      481 cgccgccgct gtctaagcgg ctggcgagac acgaaagggc gacatgttga aaatcctgct
      541 gctggctggc gacggtatcg gtccggagat catggtggag gccgaaaaag tccttggcgc
      601 gctcgatctt cccgtgacgc tcgaccgcgc gctcgtcggc ggagcggcct atgaagcgac
      661 cggccacccg ctgccccccg agacgctggc aaaggcgaaa gccgccgatg cgttgctgtt
      721 cggcgccgtc ggcgatccgc gtttcgacaa tgtcgaacgc catttgcgcc ccgaacaggc
      781 gatccttggt ctccgcgccg aactcgggct gttcgccaat ctgcgtcccg ccaaactgtt
      841 cgccgggctc gaagacagct cggcgctgcg ccccgaggtt gcgtcggcga tcgacctgct
      901 gatcgtccgc gagctcaacg gcgacgtcta tttcggcgaa aaaggtatgc gcacgacgcc
      961 aagcggcgag cgcgaaggct atgacgtcat gtcgtacagc gagagcgaag tgcggcgcat
     1021 cgcgcatgtc gcgttccgcg cggcgcaggg acgggcgaag cggctctgtt cggtcgacaa
     1081 ggcgaatgtg ctcgaaacct cgcagctctg gcgcgacgtg gtgatcgggg ttgccgccga
     1141 ttaccccgac gtggcgctga cccatatgta tgtcgacaat gccgcgatgc agctggtgcg
     1201 caacccgggc cagttcgacg tcgtcgtcac cggcaatctg ttcggcgaca ttctttcgga
     1261 tcaggcgtcg atgtgcgtcg gctcgatcgg gctgctcgcg tcggcgtcgc tgagcgaagg
     1321 caagctggga ttgtacgagc cgatccacgg tagcgcgccc gacatcgccg gcaagggcgt
     1381 tgccaacccg ctggcgatga tcctgtcgct cgcgatgttg ctgcgccatt cgggcggcga
     1441 cgaggcgagc gcggcgcgga tcgaggcggc ggtggccaag gtgctggcgg acggcgcgcg
     1501 cggtgcagat ctggggggca atatgggaac agcggcattg ggcgatgcgg ttgtttcggt
     1561 tttgaacgga tagatgagat gaaaaagacg acgggtttcg atcgcagcaa gaccaggagc
     1621 tggcatcccg caacccaggc ggtgcgcggc ggcacctggc gcagcgagca tggcgagacg
     1681 tcggaggcgc tcttcctgac ctcgggctac acctatgaga gcgccgagga agtcgcggcg
     1741 cgcttcgccg gcgaagagca gggcatgacc tattcgcggt tgcagaaccc gactgtcgcg
     1801 atgctcgagg agcggatcgc gctgatggaa ggcgccgagg cgtgccgtgt gcaggcgacc
     1861 ggcatggcgg cgatgacgac cgccttgctg tgccaattgt cggcgggcga tcatatcgtc
     1921 gcggcgaaag cggccttcgg gtcgtgccgc tggctcgtcg accatctctg tccgcgcttc
     1981 gggatcgaaa ccaccgtcgt cgacggccgc gacaatgatg cgtgggaaaa ggcgatcaag
     2041 ccgaacacca aggtcttctt tttcgaaacc ccggcgaacc cgacgatgga tatcgtcgac
     2101 atgaagcatg tctgcgcgct cgcgaaggcg cacggcatca ccaccgtcgt cgacaacgcc
     2161 tttgcgacga gcgtgctcca gcgccccatg gatttcggcg ccgacgtcgt cgcttactcg
     2221 gcgaccaagc tgatggaagg gcaggggcgc gtcctcgccg gcgccgtctg tgggaccggg
     2281 aagttcatca acgacgtgct gctgccgttc cagcgcaaca ccgggccgaa cctttcgccg
     2341 ttcaacgctt gggtcgtgct caagggcctc gaaacgctcg acctgcgcgc gcggcgccag
     2401 tcggaaaacg cgctcgcggt cggcaaggcg atcgagggcc gcgtcgcgcg catgctgcat
     2461 ccggggctcg ccagccaccc gcagcacaat ctggcgatga gccaatcgag cgcgtgcggg
     2521 ccgatctttt ccttcgtgct cgatggcgga cgcgagcagg cgatgggctt cctcaacgcg
     2581 ctcgagctcg tcgacatcag caacaatatc ggcgattcgc gcagtctctg ctgtcatccc
     2641 tggtcgacga cgcattatgg cgtcagcgaa gaggcgcgta tcgatatggg ggttgtcgag
     2701 ggcatgctgc gcatcaatat tgggctcgag gatccgcgcg acgtgatcgc cgacctcgac
     2761 caggcattga ccaaggtcgg gctttaagcg aatctgccat ggaaatgatc gactccttct
     2821 tcccctttgc ggcgaccacc gggtcgatca ccgtgcgcgt cgcggtcagc tatctgcccg
     2881 aacagtcgca ccccgcgctc gggcgctggt tctgggccta tcatgtgcgc gtcgagaacc
     2941 acggcgacga cgcggtgcag ctgatcagcc gccattggcg gatcagcgac gggcgcggcc
     3001 agaccagcga ggtcgaaggc gaaggcgtcg tcggcgaaca gccgttgatc atgcccggcg
     3061 gcgcttatga ctatgtctcg ggctgcccgc tgccgacccc cgaagggtcg atggtcggca
     3121 gctatgcgat ggagctgggc gacgggtcgc agatgctcgt cgccatcccg catttcccgc
     3181 tcgtggcgcc cgcgaccgcg tcatgaagcg cacgcacctg cccctgaacg ccctgcgcgt
     3241 gttcgacgcc gctgcccggc atctgagctt cacgcgcgcc gccgacgagc tggcggtaac
     3301 ccccgccgcg gtggggcagc agattcgcgc gctcgaggat atgctcggcg tcgtgctgtt
     3361 ccgccgcacg ccgaagggac tcgagctgac gggcgaggcg aatgccgggc tcgacgcgct
     3421 gcgccagggt tttttgcagt tcgaagaatc ggtgcgcgcg atgcaggcgg gccagtcgtc
     3481 gcagtcgctg acgatcgccg cgccgcgcga cctgaccgcc aaatggctcg cgccgcggct
     3541 cgcgcgctac agccagcaag cgcccgacgt gcgcttcacc ttggtatcgg ccgacagcgg
     3601 gatcgatttt accgaggcga acctcgacct cgcgatcttc tggaccgatg gcgcgggcga
     3661 acatgagggc gtggcgctgg ccgaagcgat gatggtgact gttgcgggac ccgccagcga
     3721 aagcgagacg cgcatcgcct ggcccggctg tcccgtcgag gacggcgaac cggcgttgcg
     3781 gc
//
LOCUS       DATMSU010000191         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_60440, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000191 DATMSU010000000
VERSION     DATMSU010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60440"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /gene="dmeF"
                     /locus_tag="VN034_04900"
     CDS             complement(<1..740)
                     /gene="dmeF"
                     /locus_tag="VN034_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HWV59966.1"
                     /translation="MSESPSTQHLHDFLGASHDENAKRTLWVVALTAVMMVAEIAAGY
                     WTGSMALLADGFHMATHAGALGLAALAYRYAKKHRHDPRYSFGTGKVGDLTGFASALI
                     LGIFAIGIAVESFLRLIDPVRVDFASATLVAVLGLAVNVASALLLMGGHHHHGHEHDH
                     DHDHHHHHGHDNNLRSAYFHVLADALTSLLAIGALLAGRYLGLHWMDPAMGIVGALVI
                     ARWSWGLMRDTAAILLDTNDDEAAAAIRA"
     gene            complement(780..1202)
                     /locus_tag="VN034_04905"
     CDS             complement(780..1202)
                     /locus_tag="VN034_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV59967.1"
                     /translation="MQQQISVITLGIANLARSRRFYVDGFGWSPVFESEEIIFYQMNG
                     LMLGTFLQASLEEDMNRRGLVRPGAFALAHNVESAGAVDAAIDRLLAAGGALLRTPDA
                     PPPGGYRGYVSDPDDHAWEIAWNPAWSIDGEGRVAFGL"
     gene            complement(1400..>2998)
                     /locus_tag="VN034_04910"
     CDS             complement(1400..>2998)
                     /locus_tag="VN034_04910"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HWV59968.1"
                     /translation="VIARAKAFLDEAVPLASGSWADWQGGQLSLADPAQWAGSKPGGI
                     LLRHNGLHIELVIDPDSAIGKTDPAGIADVLLEAALTTIIDLEDSVAAVDGEDKALGY
                     TNWLGLMRGDLVESFDKGGKTVTRAMAADREWTSPSGEAFTLHGRSVMFVRNVGHLMT
                     TPMIKLPDGAEAPEGLCDAVITSLCSLHDLKGLGSLRNSRAGSIYIVKPKQHGPEECG
                     FTDRVFNAVEDMLGLARHTIKVGVMDEERRTSTNLAACIHAVKDRIVFINTGFLDRTG
                     DEIHTSMRAGPMIPKGEMKASDWIASYEDRNVRIGLACGLSGKAQIGKGMWAMPDLMR
                     GMLDAKIGHPKSGANTAWVPSPTAATLHALHYHQVDVFARQQEIAGEAVPSLDRLLAI
                     PVATGRNWSEAEITRELDNNCQGILGYVVRWIDQGVGCSKVPDIDDVGLMEDRATLRI
                     SSQALANWLLHGVCTAEQVDAALARMAAKVDAQNAGDPLYEKLTPDGIAHRAARALIF
                     EGVTQPSGYTEPLLHKYRQRKKAG"
BASE COUNT          492 a          968 c         1023 g          515 t
ORIGIN      
        1 gcgcggatcg ccgccgcggc ctcatcgtcg ttggtgtcga gcaggatcgc ggcggtgtcg
       61 cgcatcagcc cccacgacca gcgcgcgatc accagcgccc cgacgatgcc catcgcgggg
      121 tccatccagt ggagcccgag atagcgcccc gcgagcagcg cgccgatcgc aagcaatgag
      181 gtcagcgcgt cggcgaggac gtggaaatag gccgagcgaa ggttattgtc gtgaccgtgg
      241 tggtggtggt ggtcgtgatc atggtcatgc tcgtgcccgt gatggtgatg cccgcccatc
      301 aagagcagcg cgctcgcgac attgaccgcg agcccgagca ccgcgacgag cgtcgcgctc
      361 gcgaaatcga cccgaacggg gtcgatcaga cgcaggaagg attcgaccgc gatgccgatc
      421 gcgaaaatgc cgaggatcag cgccgaggca aaaccggtga ggtcgccgac cttgccggtg
      481 ccgaagctgt agcgcggatc gtggcgatgt ttcttggcat agcgataggc gagcgcggcg
      541 aggccgagcg cgcccgcatg cgtcgccata tggaagccgt cggcgagcag cgccatcgac
      601 ccggtccagt aacccgccgc gatttccgca accatcatca ccgcggtcag cgcgacgacc
      661 cacagcgtgc gcttcgcatt ctcgtcgtgc gaggcgccga ggaagtcgtg cagatgctgg
      721 gtggatgggg attcggacat gcccctaccc tataaataat cagctctccg gcaagacgat
      781 caaagcccga aggcgacgcg tccttcgccg tcgatggacc aggcgggatt ccaggcgatt
      841 tcccaagcat gatcgtcagg gtcggagaca tagccgcgat agccacccgg cgggggcgca
      901 tcgggggtgc ggagcaaggc gccccccgcg gctagaagcc ggtcgatcgc ggcgtcgacc
      961 gcacccgcgc tttcgacatt gtgcgcgagc gcgaaggccc ccgggcgaac tagcccgcgg
     1021 cggttcatat cctcctccag cgaagcttgc aggaaggtgc cgagcatgag gccgttcatc
     1081 tggtagaaga tgatctcctc actttcgaac accggcgacc agccgaagcc atcgacgtag
     1141 aagcgccgcg agcgcgcgag gttcgcgata ccgagcgtga taaccgaaat ctgctgttgc
     1201 atgacacgac ctttcgacgc ggcgacgcga agatgcgccg ccgttccagg tcatggggct
     1261 cgccgccgaa cggctgccag gacccgcgag cgcgggggcc gggccaaccg caccgaccta
     1321 tccttttctg acggggtcat cttatcgacg cccgggcgcg cgttcaaccg gcggcggagc
     1381 aaagccccgc gccgatagct cagcccgcct ttttcctctg ccggtatttg tgcagcagcg
     1441 gttcggtata gcccgagggt tgcgtcacgc cttcgaagat cagcgcgcgc gcggcgcggt
     1501 gggcgatacc gtcgggggtg agtttttcgt agagcggatc gccggcgttc tgcgcgtcga
     1561 ccttggccgc catgcgcgcc agcgcggcgt cgacctgttc ggcggtgcag acgccgtgga
     1621 gcagccagtt ggcgagcgcc tggctcgaaa tgcgcagcgt cgcgcggtct tccatcaggc
     1681 cgacgtcgtc gatgtcgggg accttcgaac agccgacgcc ctgatcgatc cagcgcacga
     1741 catagccgag gatgccctgg caattattgt cgagttcgcg ggtgatttcg gcttcgctcc
     1801 agttgcgtcc cgtcgcgacg gggatggcga gcaggcgatc gagcgacggc accgcctctc
     1861 cggcgatttc ctgctgccgc gcgaagacat cgacctgatg atagtggagc gcgtgcaagg
     1921 tcgcggcggt cggcgacggc acccacgcgg tgttcgcgcc cgatttcgga tggccgatct
     1981 tggcgtcgag catgccgcgc atcaggtcgg gcatcgccca catgcccttg ccgatctgcg
     2041 ccttgcccga aagcccgcag gcgaggccga tgcggacgtt gcgatcctcg tacgaagcga
     2101 tccagtccga cgccttcatc tcgcccttgg ggatcatcgg ccccgcacgc atgctggtgt
     2161 ggatttcgtc gccggtgcgg tcgaggaagc cggtgttgat gaagacgatg cggtccttca
     2221 ccgcgtggat gcacgcggca aggttcgtgc tggtgcggcg ctcctcgtcc atcacgccga
     2281 ccttgatcgt gtggcgcgcg aggccgagca tgtcctcgac ggcgttgaaa acacgatcgg
     2341 tgaagccgca ttcctcgggc ccgtgctgct tgggcttgac gatatagatg ctgcccgcgc
     2401 ggctgttcct gaggctgccg aggcctttca ggtcgtggag agaacagagg ctggtgatca
     2461 ccgcgtcgca gaggccttcg ggtgcttcgg cgccgtccgg cagcttgatc atcggcgtcg
     2521 tcatcagatg gccgacgttg cgcacgaaca tcacgctgcg gccgtgcagc gtgaaggctt
     2581 cgcccgaggg cgaagtccat tcgcggtcgg ccgccatcgc gcgggtcacc gtcttgccgc
     2641 ccttgtcgaa actttcgacg aggtcgccgc gcatcaggcc gagccagttg gtgtagccga
     2701 gcgccttgtc ctcgccgtcg accgcggcga cgctgtcctc aaggtcgatg atcgtcgtca
     2761 gcgcggcttc gagcaggacg tcggcgatgc ccgccggatc ggtcttgccg atcgcgctat
     2821 cggggtcgat caccagttcg atgtgtaggc cgttgtggcg gagcaggatg ccgccgggct
     2881 tgctgccggc ccattgcgcg gggtccgcaa ggctgagctg cccaccctgc cagtcggccc
     2941 agcttcccga agcgagcggc accgcttcgt cgaggaatgc cttggcgcgc gcgatcac
//
LOCUS       DATMSU010000192         3915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_155143,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000192 DATMSU010000000
VERSION     DATMSU010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155143"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..898
                     /locus_tag="VN034_04915"
     CDS             146..898
                     /locus_tag="VN034_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59969.1"
                     /translation="MSRISGRPAISSEHVYGLSLRVARWREGTPGTPLLFLNGIGADL
                     AAAAPLLAQIHGREVWTLDMPGVGGSPDALLPYAAPTIAAVVMKIAGRLEHKVIDVAG
                     FSWGGALAQQVAIQFPGRVRRLVLMATTPSIGALGIGWAALLDDDMLASGLKLPTATP
                     LGLAYQSMAMAGWTSAAMLPHLKGTPVLILMGERDGVVPACHGQAIADLIDGAVLEVV
                     PGSHLFPFTHAAAISARISAFLDQPAKVQAAA"
     gene            complement(852..1892)
                     /locus_tag="VN034_04920"
     CDS             complement(852..1892)
                     /locus_tag="VN034_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV59970.1"
                     /translation="MIADDMDFDERPPRRRPLFRRKRVLIPLGVVLAIIVGFLLLLTP
                     DTDRDAMIAKYGGPNAAFVAGPAGQRIHYRDQGRADGPPIILIHGANASLHTWEPLVK
                     RLGATWRIVTLDLPGHGLTGATPDKDYSAEGMMNAVDVVAAKLGLDHFVLGGNSMGGW
                     VAWRYALAQPDRVDALLLIDAAGMPLRKGEKRPESNVGFRILEYPFGRWLATRVTPRM
                     LVEQSLRGSVERQDIVDEAMIDRYWELLLFPGNREATVLRARMDREPEIAARVGEITA
                     PTLILFGDKDRVINPGAAKTFNERIQGSEVVMLPGIGHLPMEEAPDATAAAIADFLKR
                     RLAPSPADPETR"
     gene            complement(1949..3619)
                     /locus_tag="VN034_04925"
     CDS             complement(1949..3619)
                     /locus_tag="VN034_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV59971.1"
                     /translation="MTDGAGAATAINTRQSERKVILASSLGTVFEWYDFYLYGLLATI
                     ISAQFFSGVNETTGFIFALAAFAAGFAVRPFGALVFGRIGDLVGRKNTFLVTMGLMGA
                     STFLVGVLPSYASIGVAAPILLVLLRLVQGLALGGEYGGAATYVAEHAPDGKRGLFTS
                     FIQTTATLGLFAALGVVIVIRSAMGEAAFADWGWRIPFLISIILLGVSLWIRLQLEES
                     PVFKQMKEEGTTSKAPLTEAFGRWENLKWVLVALFGAVAGQAVVWYSGQFYALFFLEK
                     TLKVDGATANILIAIALALGTPFFILFGWLSDKVGRKPIILAGCALAAITYFPAFHML
                     TEAANPGLAAAQARAPVTVIANPAACSFQFDPIGKNRFDRTACDIAKSHLAKAGVPYV
                     SQDAGGGWATVSIGQKAITIPDPSGGIGPWQNARGIPGKLAEMDAALAAAGYPAKADP
                     AAINKPLVVAILFYLVLLVTMVYGPIAALLVELFPSRIRYTAMSLPYHIGNGWFGGFL
                     PTTAFAMVAATGNIYYGLWYPVVIAVITLVIGLLFLPETFRRSLHPPA"
BASE COUNT          681 a         1276 c         1329 g          629 t
ORIGIN      
        1 ctgctcgcgt gcaccggctc ggtgttcctt gccggggcga agattttcga ttgcctgcgt
       61 tacgagcaga gcgcggaggc cgcgcggcgg gcggaataga tcagcggtct ttcgccggtc
      121 acccagatat gcgaaggcac cggccatgag caggatcagc ggccgcccgg ccatcagcag
      181 cgagcatgta tacggtctca gcctgcgcgt tgcgcgctgg cgcgagggta cgccgggcac
      241 gcctttgctg ttcctcaacg gcatcggtgc cgatctggcc gccgccgcgc cattgctcgc
      301 gcagatccat ggccgcgagg tgtggacgct cgacatgccc ggcgtcggcg gctcgcccga
      361 tgcgctgctt ccctatgccg caccgacgat cgcggcggtg gtgatgaaga tcgccggccg
      421 gctcgagcac aaggtgatcg acgtcgcggg gttcagttgg ggcggcgcgc tcgcgcagca
      481 ggtcgcgatc cagtttcccg gccgtgttcg ccggctggta ctgatggcga cgacgccgag
      541 catcggcgcg ctggggatcg gctgggcggc tttgctcgac gacgatatgc tggcgagcgg
      601 cctcaaactg ccgaccgcca ccccgctcgg cctcgcctat caatcgatgg cgatggcggg
      661 gtggacgagc gccgcgatgc tgccgcacct caaaggcacg cccgtgctga tcctgatggg
      721 tgagcgcgac ggcgtcgtac ccgcgtgcca cggccaagcg atcgccgacc tgatcgacgg
      781 cgcggtgctg gaagtagtgc cgggatcgca cctcttcccc ttcacccacg ccgcggcgat
      841 ttcggcgcgg atcagcgcgt ttctggatca gccggcgaag gtgcaagccg ccgcttgagg
      901 aagtcggcga tcgccgccgc ggtcgcgtcg ggcgcttcct ccatcggcag atggccgatg
      961 cccggcagca tcacgacctc cgacccctgt atccgttcgt tgaaggtctt cgccgcgccg
     1021 ggattgatca cgcggtcctt gtcgccgaac aggatcagcg tcggcgccgt gatctcgccc
     1081 acccgcgccg cgatttcggg ctcgcgatcc attcgcgcgc ggagcaccgt ggcttcgcga
     1141 ttgccgggga aaaggagcag ctcccaatag cggtcgatca tcgcctcatc gacgatgtcc
     1201 tgcctttcga ccgagccgcg cagcgactgt tcgacgagca tccgcggcgt cacccgcgtc
     1261 gccagccagc ggccgaacgg atattcgagg atgcggaagc caacgttcga ttcgggccgt
     1321 ttttcgcctt tgcggagcgg catgccggcg gcgtcgatca gcagcagcgc atcgacgcgg
     1381 tcgggctgcg cgagcgcata gcgccacgcg acccagccgc ccatcgaatt gccgccgagg
     1441 acgaaatgat cgaggccgag cttcgccgcg acgacatcga cggcgttcat catcccttcg
     1501 gccgaataat ccttgtcggg cgtcgcgccg gtcagtccat gcccgggcag gtcgagcgtg
     1561 acgatgcgcc aggtggcgcc gagccgcttc accagcggtt cccacgtatg gaggctggca
     1621 ttcgcgccgt ggatcaggat gatcggcgga ccgtcggccc ggccctgatc gcggtagtgg
     1681 atacgctgcc cggccggccc cgcaacgaag gccgcgttcg ggccgccata tttggcgatc
     1741 atcgcgtcgc gatcggtgtc gggggtcagc agcagtagga agccgacgat gatcgcaagc
     1801 acgacgccga gcgggatcaa cacccgcttg cgccggaaca agggacgccg ccgcggcggc
     1861 cgctcgtcga aatccatatc gtcagcaatc atcacgcgcc cccctcaagg cgtaaagcga
     1921 ccctagcgcg cgaggacgcg gggtcaatct atgccggcgg atggagcgag cggcgaaagg
     1981 tctcgggcag gaacaggaga ccgatcacga gcgtgatcac cgcgatgacg accggatacc
     2041 agaggccata atagatatta cccgtcgccg cgaccatcgc gaacgctgtt gtcggcagga
     2101 agcccccgaa ccagccgttg ccgatatgat agggcagcga catcgccgta tagcggatac
     2161 ggctcgggaa cagctcgaca agcagcgcgg cgatcgggcc gtagaccatc gtcacgagca
     2221 gcacgagata gaagaggatc gcgacgacca gcggcttgtt gatcgcggcg gggtcggcct
     2281 tggcgggata gccggcggcg gcgagcgccg cgtccatctc ggccaacttg ccgggaatgc
     2341 cgcgcgcgtt ctgccaaggg ccaataccgc ccgacggatc ggggatcgtg atcgcctttt
     2401 gtccgatgga gaccgttgcc cagccgcccc ccgcgtcttg cgaaacataa ggcacgccgg
     2461 ccttggccag atgcgatttc gcgatgtcgc aggcggtccg gtcgaacctg ttcttgccga
     2521 tcggatcgaa ctggaacgag caggcggcgg ggttggcgat caccgtcacc ggcgcccgcg
     2581 cctgtgccgc cgcgagcccc gggttggcgg cttcggtcaa catgtggaac gccgggaaat
     2641 aagtgatcgc ggcgagcgcg cagcccgcga ggatgatcgg cttgcgcccg accttgtcgc
     2701 tgagccagcc gaagaggatg aagaagggcg tgccgagcgc gagcgcgatg gcgatcaata
     2761 tattcgcggt cgcgccgtcg accttcagcg tcttttcgag gaagaagagc gcatagaatt
     2821 gccccgaata ccagacgacc gcttgccccg cgaccgcgcc gaacagcgcg acgagcaccc
     2881 atttgagatt ttcccagcgg ccgaaggctt cggtgagcgg cgccttcgac gtcgtcccct
     2941 cctccttcat ctgtttgaag acggggcttt cctcgagctg gaggcggatc cagagcgaga
     3001 cgccgagcag gatgatcgag atcaggaagg ggatgcgcca gccccagtcg gcgaacgccg
     3061 cctcgcccat cgccgagcgg atgacgatga cgacgccgag cgccgcgaac agccccaatg
     3121 tcgcggtggt ctggatgaag ctggtgaaaa ggccgcgttt gccgtcgggc gcatgttcgg
     3181 cgacataagt ggcggcgccg ccatattcgc cgccgagcgc gagcccctgg acgagacgga
     3241 gcagcacgag gagaatcggt gccgcgacgc cgatcgacgc atagctgggc agcacgccga
     3301 cgaggaaggt cgaggcgccc atcagcccca tggtgacgag gaaggtgttc ttgcgcccga
     3361 cgaggtcgcc gatgcgcccg aagacgagcg cgccgaacgg ccttaccgca aagcccgccg
     3421 caaaggccgc gagcgcgaag atgaagccgg tggtttcgtt gacgcccgag aagaattgcg
     3481 ccgaaatgat cgtcgcgagc aggccgtaga gatagaaatc ataccattcg aagaccgtgc
     3541 cgagcgatga tgcgaggatg accttgcgct cgctctgccg ggtgttgatt gctgttgctg
     3601 cgcctgcgcc gtccgtcatt cactgcccct taacccacat aataagcccc tccccttcag
     3661 gggaggggtt ggggtggggg ctcgaagtct tgcgccagac cgatgccccc accccgctcg
     3721 actaaggctc ggctgacgcc gaaccaagtc tcactgcccc tcccctgaag gggaggggtt
     3781 tgggttagcc tagtggatga acttccggcg tcaaccgacg cgcgtcaccg cacccttgtg
     3841 cgctccgacg ctcttgttcc gcgggcggaa gcggcgcttg cgctgcggtt ggccttcgcc
     3901 ggccggagcg tcgcg
//
LOCUS       DATMSU010000193         6328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145416,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000193 DATMSU010000000
VERSION     DATMSU010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145416"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..994)
                     /locus_tag="VN034_04930"
     CDS             complement(110..994)
                     /locus_tag="VN034_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWV59972.1"
                     /translation="MKAVHQNYQARMQRVLDHIDQHLDDELDLDAVSRVAAFSKFHFH
                     RQFTATFGLSVHRYIQLARMKRASHRLTSQEKQSITEIAMDAGYDAPDAFARAFRQRF
                     GQSPSSFRKSPDWAPWLAAFGPLNNAKGKLMQIKYEYDDVTIREVASTPVAVLEHRGD
                     RAMLPTTIERFIAWRKAAGLSPATSPTFNIFRSERTPVNPADYSMDLCVGTDREIAPD
                     DEVMKPGAIPGGRCAVLRVVHDTHNLEPAALFLYGDWLPASGEEARDFPIYCRRHLNF
                     FPDAAVQDVVVELFLPLK"
     gene            complement(1142..2167)
                     /locus_tag="VN034_04935"
     CDS             complement(1142..2167)
                     /locus_tag="VN034_04935"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWV59973.1"
                     /translation="MLDGFASREARAAFIARLPKAELHLHIEGSLEPELMFELAQGNR
                     VAIPFASVEEVRAAYAFSNLQDFLDIYYQGMGVLHREQDFYDLTAAYLARANADNVRH
                     VEIFFDPQGHTERGVAFGTVIAGITRALDDAEASYGMTSKLIMCFLRHLSEAEAQATL
                     DAALPYLDRIDGVGLDSSEVGHPPSKFERVFARARGLGLKIVAHAGEEGPPEYVREAL
                     DLLKVDRIDHGNRSLDDPALVARLAAEGMTLTVCPLSNLKLCVVDDIADHPLKTMLDA
                     GLKATVNSDDPSYFGGYVNANYQAVADALDLSKAELVTLARNSFTGSFLSESDKAGHL
                     AAIDAHA"
     gene            complement(2160..2735)
                     /locus_tag="VN034_04940"
     CDS             complement(2160..2735)
                     /locus_tag="VN034_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWV59974.1"
                     /translation="MTAEKIFISANDLLADSLRLGMQVIESGFKPTHLVGIWRGGAPV
                     GIAVQELLDYHGHHCDHIAIRTSSYKGIDRQDPEVKVFALGYLIDTLNPDDRLLIIDD
                     VFDSGRSIRAFIAELKARCRHNMPRDIRIATVWFKPGRNVTDLRPDFFVHETDRWLIF
                     PHEIDGLTPEEIRRHKPEAAIILGEAQVPGA"
     gene            2947..3615
                     /locus_tag="VN034_04945"
     CDS             2947..3615
                     /locus_tag="VN034_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HWV59975.1"
                     /translation="MSDQLVLTAETRERGGKGASRDLRREGRVPAVVYGGKEEPLMIH
                     VEEKLLMKQLMTGHFMNSVVMIDVGGKQIRTLPKDVAFHPVKDRPIHADFLRIAKDAT
                     VQVAVPVSFLNEEASPGLKRGGVLNIVRHELELVCDADKIPDQIAIDVTGFDVGDSIH
                     ISNVTLPKGVESAITDRDFTIATIVAPSALKSSDGDTTKDEGEAAEGEAAEGEAPAAE
                     GDEA"
     gene            3685..4254
                     /gene="pth"
                     /locus_tag="VN034_04950"
     CDS             3685..4254
                     /gene="pth"
                     /locus_tag="VN034_04950"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWV59976.1"
                     /translation="MQLWVGLGNPGPKYAMHRHNVGFMAADVIADLHDFPPPAKKFQG
                     WIQEGRIGSQRILLLKPGTFMNESGRSVRAALDFYKLTPQDVTAFYDELDLAPMKVKV
                     KQGGGAAGHNGIRSMISHIGEDFRRIRIGIGHPGHKDRVTGHVLGNYHKSEMEPLIDL
                     LGAIAAEAKWLADGDDVRFQSDLALRLQG"
     gene            4427..6301
                     /locus_tag="VN034_04955"
     CDS             4427..6301
                     /locus_tag="VN034_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HWV59977.1"
                     /translation="MSHLTDKARAPYRGETGGLPGPDSLEAIVAENPTRRTLLRNGLF
                     GLSVIPAMALAGCDDDGEPIVTVPPTPTPTPTPTPTPPPSFAANFAAVAANQDDRVTV
                     PEGYSVDVLLKAGDSVEAGTAYSGSFPASPAVAEKWAGGGHDGMEYFALPDVDANTRG
                     LLAMNYEYPDFNILMADGYDAATASADQKAIALSAVGIGVVEIAKGSDGKWTVQAGST
                     YNRRYTGNSSYRAGGPAAGLLSGSIKGMLNNCSSGRTPWSTYLTCEETMDNYLDPAQP
                     ETNYGWVVEIDPLRELAVPTKRTAMGRFNHENVAFMANTDRRVAFYMGDDTTPGCIYK
                     FVPDRAYSDTNRAANTDLLDYGTLYVARFNGDGTGEWRALVVGQNGLTAGASDPGNVS
                     QSETPPTPTTVDFNNQADVLINCQRAARVAGGTVMDRPEWLTVAPDNSAVFCTLTNNS
                     GRRVADPANPRIRNLHGHILKFSEEGDSPLATKFSWEIFLTAGDPSLAAGGSNLVGDI
                     EGDTFSSPDGLRIDPEGRMWVQTDHSVPGNSGLDGTTIDQAFGHNAMFYVDQQNKQSK
                     RFLVGPLGCEITGLAYTPDLTTFFVNIQHPTGDWPVAGEAPRSSTIVVRRTDNQPVGN
                     "
BASE COUNT         1168 a         2047 c         2061 g         1052 t
ORIGIN      
        1 cccgtcgtgg tcgccgacga cgcgctgatc tgcgtcgcga tgggcgcggg gcgcgcgttc
       61 gaggaccgca cctatcacgg cgtgctgatc gcggcctgag ccgcccgcat tatttcagcg
      121 gcaggaacag ttccacgacg acgtcttgca cagctgcgtc cggaaagaaa ttgaggtgcc
      181 gcctgcaata gatagggaag tcgcgggctt cctcgccgct tgcgggaagc cagtctccgt
      241 aaagaaagag cgcggcgggc tccagattgt gggtgtcatg gacgacgcgc aggaccgcgc
      301 agcgcccgcc ggggatcgcg ccgggcttca tgacctcatc atcaggcgcg atctcgcggt
      361 ccgtcccgac gcaaaggtcc atgctgtagt cggccgggtt taccggcgtc ctttcggacc
      421 ggaagatatt gaaggtcggg ctcgtcgcgg gcgaaagccc tgcggccttg cgccatgcga
      481 tgaaccgctc gatcgtggtc ggcagcatgg cgcggtcgcc gcgatgttcg aggaccgcca
      541 ccggcgtcga agctacttcg cggatcgtca cgtcgtcata ttcgtatttt atctgcatca
      601 gcttgccctt cgcattgttg agaggcccga aggccgcaag ccacggtgcc cagtcgggcg
      661 atttccggaa cgacgacggc gattgtccga accgctgccg gaaggcgcgg gcgaaggcat
      721 cgggtgcgtc gtaaccggca tccatcgcta tctccgtaat gctctgcttc tcttgcgagg
      781 tcagccggtg cgaggcccgc ttcatgcggg ccagctggat atagcgatgc acggacagcc
      841 cgaaggtcgc cgtgaactgc cggtggaaat gaaatttcga aaaggcggcg acccggctta
      901 ccgcgtcgag atccagctcg tcgtcgagat gctggtcgat atggtcgagc acccgctgca
      961 tccgggcctg ataattttgg tgcaccgcct tcatcattcg ctcctgcgtg gcggcaccca
     1021 ttagacgcaa tggcgcgcgc accgctcgac cgatcttgcg ctttgggcat ggatcggttc
     1081 ggtcgaatcc gacgtgaaac gactgcaacc ggcagccagt cgacgcctct gagtttgccg
     1141 atcaggcgtg ggcgtcgatc gcggcaaggt gtccggcttt gtcggattcg ctcaggaaag
     1201 agccggtaaa gctgttgcgc gcgagcgtga cgagttcggc cttcgacagg tcgagcgcgt
     1261 cggcgaccgc ctgataattg gcgttcacat agccgccgaa atagctggga tcgtcgctgt
     1321 tcaccgtggc tttgaggccc gcgtcgagca tggtcttcag cggatggtcg gcgatgtcgt
     1381 cgacgacgca gagcttcaga ttcgacagcg ggcagacggt gagtgtcatg ccctcggccg
     1441 cgaggcgcgc gacgagcgcg gggtcatcga ggctgcggtt gccatggtcg atgcggtcga
     1501 ccttcaagag gtcgagcgct tcgcgaacat attcgggcgg cccctcctcg ccggcgtgcg
     1561 cgacgatctt gaggccaagg ccgcgggcgc gggcgaagac gcgttcgaat ttggacggcg
     1621 gatggccgac ttcggacgag tcgagcccga cgccgtcgat gcggtccaga tatggcagtg
     1681 ccgcgtcgag cgtcgcttgg gcctcggctt cgctgaggtg gcggaggaag cacatgatga
     1741 gcttcgacgt catcccgtaa cttgcttcgg catcgtcgag cgcgcgcgtg atgcccgcga
     1801 taacggtgcc gaaggcgacg ccccgctccg tgtgtccctg cggatcgaag aaaatctcga
     1861 cgtgccggac attgtcggcg ttggcgcgcg cgaggtaggc ggcggtcagg tcgtaaaaat
     1921 cctgctcgcg gtggagcacg cccatgccct gataatagat gtcgagaaaa tcctggaggt
     1981 tcgaaaaggc ataggccgcg cgcacctcct cgacgctcgc gaaggggatg gcgacacggt
     2041 tgccctgcgc gagttcgaac atcagctcgg gttcgagcga gccctcgata tggaggtgaa
     2101 gctcggcctt gggcagccgg gcgatgaagg cggcgcgcgc ctcgcgcgag gcgaaaccgt
     2161 caagcacccg gcacctgcgc ttccccgagg atgatcgccg cctcgggctt gtggcggcgg
     2221 atttcctcgg gcgtcaggcc gtcgatctcg tgcgggaaga tcagccaccg gtcggtttcg
     2281 tggacgaaga aatcggggcg caggtcggtg acgttgcggc cgggcttgaa ccacacggtc
     2341 gcgatgcgga tgtcgcgcgg catattgtgg cggcagcgcg ccttgagctc ggcgatgaag
     2401 gcgcggatgc tgcgcccgct gtcgaacaca tcgtcgatga tgagcagccg gtcgtcggga
     2461 ttgagcgtgt cgatcagata gccgagggcg aagaccttta cctcaggatc ctgccggtcg
     2521 atgcctttgt aggaggaggt gcggattgcg atatggtcgc agtggtggcc gtgatagtcg
     2581 agcaattcct gcaccgcgat cccgaccggc gcgcccccgc gccagatgcc cacgagatgc
     2641 gtcggcttga aaccgctttc gatgacctgc atcccgaggc gtagcgagtc ggccaggaga
     2701 tcgttcgcgc tgatgaagat tttctcggcg gtcatgaggt gcggagtaat cctccggggc
     2761 gaaggccgca agacgcggcg gctttggggt ggcgagcggc caccttcctc ttgccgccga
     2821 cgaatgcttg caattccgcc gcttcccggc taagcgccgc ggcttccgtc atggtcatcc
     2881 ctggaggcgt ggcggaactt tgtaacccag ggtcataggt tctgacccta atctcggaga
     2941 aatattatga gcgatcagct tgtgctgacg gccgagacgc gcgaacgcgg aggcaaggga
     3001 gcctcgcgtg acctgcgtcg tgaaggccgt gtccccgccg tcgtctatgg cggcaaggaa
     3061 gaacccctga tgatccacgt cgaggaaaag ctgctgatga agcagctgat gacgggtcac
     3121 ttcatgaact cggtcgtgat gatcgacgtc ggcggcaagc agatccgcac gctgcccaag
     3181 gacgtcgcgt tccacccggt caaggatcgc ccgatccacg ccgacttcct gcgcatcgcc
     3241 aaggacgcca ccgtccaggt cgccgtgccg gtctcgttcc tgaacgaaga agcctcgccc
     3301 ggcctgaagc gcggcggcgt gctgaacatc gttcgccacg aactcgaact cgtctgcgac
     3361 gcggacaaga ttcccgacca gatcgcgatc gacgtcaccg gcttcgatgt cggcgattcg
     3421 atccacatca gcaacgtcac cctgcccaag ggcgtcgaaa gcgcgatcac cgaccgcgac
     3481 ttcaccatcg ccaccatcgt cgccccgtcg gcgctcaagt cgagcgacgg cgacacgacg
     3541 aaggacgagg gcgaagctgc cgaaggcgaa gcagccgaag gcgaagcgcc cgccgccgaa
     3601 ggcgacgaag cctaatatcg ttcggacggc ccctttccgc gcggaaaggg gccgccaacg
     3661 acaggacccg ggaaaggcag tatcatgcag ctctgggtcg gcctcggcaa tcccgggccc
     3721 aaatatgcga tgcaccgtca caatgtcggc ttcatggccg ccgacgtcat cgccgacctc
     3781 cacgacttcc ctccgcccgc caagaaattc cagggctgga tccaggaagg ccgcatcgga
     3841 tcgcagcgca tcctgctgct caaacccggc accttcatga acgaaagcgg ccgcagcgtg
     3901 cgcgccgcgc tcgatttcta caaattgacg ccgcaggacg tcaccgcctt ttacgacgag
     3961 ctcgacctcg ccccgatgaa ggtgaaggtg aagcagggcg gcggtgcggc tggccacaac
     4021 ggcatccgca gcatgatcag ccacatcggc gaggatttcc gccgcatacg catcggcatc
     4081 gggcatccgg ggcacaagga ccgcgtgacc ggccacgtcc tcggcaatta tcacaagagc
     4141 gagatggaac cgctgatcga cctcttgggc gcgatcgccg ccgaagcgaa atggctcgcc
     4201 gacggcgacg atgtgcgttt ccagagcgat ctggcgctgc gtttgcaggg ctgacgagcc
     4261 ctacaccccg acggccggct ccgaccgctg tcacatgccc gtcatataag tcattgcatt
     4321 ccgacgcgtt cgagcaacgc ggctgacatc atcctctgca atcggtgcgc cacctcccaa
     4381 ccgggacaat cgagtcccgg ggctcaaccg aaaacagggg aactgcatgt cacatcttac
     4441 cgacaaagcc cgtgcgcctt atcgcggcga aaccggcggc cttccgggtc ccgacagcct
     4501 cgaggcgatc gtggccgaaa atccgacgcg ccgcacgctg ctcaggaacg gcctgttcgg
     4561 cctgtccgtg atccccgcga tggcgctcgc cggatgcgac gacgacggcg aaccgatcgt
     4621 gacggtcccg ccgacgccga ccccgacgcc gacccccacc ccgaccccgc cgccgagctt
     4681 cgccgccaat ttcgccgcgg tcgccgcgaa ccaggacgac cgggtgacgg tgcccgaggg
     4741 ttatagcgtc gacgtgctgc tgaaagcggg cgattcggtc gaggccggca ccgcctattc
     4801 gggcagtttc cccgcgagcc ccgcggtcgc cgagaaatgg gcgggcggcg gccacgacgg
     4861 catggaatat ttcgcgctcc ccgacgtcga tgccaacacc cgcggcctgc tcgcgatgaa
     4921 ctatgaatat cccgacttca acatcctgat ggcggacggc tacgacgccg cgacggcgag
     4981 cgccgaccag aaggcgatcg cgctgtcggc ggtcggcatc ggcgttgtcg aaattgccaa
     5041 gggcagcgac ggcaaatgga cggtccaggc gggatcgacc tataaccgcc gctacaccgg
     5101 caacagcagc tatcgcgccg gcggccccgc cgcgggcctg ctgtcgggca gcatcaaggg
     5161 catgctcaac aattgttcga gcggacggac gccttggagc acctacctca cctgcgaaga
     5221 gacgatggac aattatctcg acccggcgca gcccgaaacc aactatggct gggtggtcga
     5281 gatcgacccg ctgcgcgaac tcgcggttcc gacgaagcgc accgcgatgg gccgtttcaa
     5341 ccacgagaat gtcgccttca tggcgaatac cgaccgccgc gtcgctttct atatgggcga
     5401 cgacacgacc ccggggtgca tctataagtt cgtccccgac cgcgcctata gcgacaccaa
     5461 tcgcgccgcc aacaccgatc tgctcgatta tggcacgctc tatgtcgcgc gcttcaacgg
     5521 cgacggcacg ggcgaatggc gcgcgctggt cgtcggccag aacggcctga ccgcgggcgc
     5581 ctcggacccg ggcaatgtca gccagagcga gacgccgccg acgccgacga cggtcgattt
     5641 caacaaccag gccgacgtgc tcatcaactg ccagcgcgcg gcgcgcgtcg ccggcggtac
     5701 ggtgatggac cgccccgaat ggctgaccgt cgcgcccgac aacagcgcgg tgttctgcac
     5761 gctgaccaac aacagcggcc gccgcgtcgc cgaccccgcc aacccgcgga tccgcaacct
     5821 gcacggccat attctgaagt tcagcgaaga aggcgattcg ccgctggcga ccaagttcag
     5881 ctgggaaatc ttcctgaccg cgggcgatcc gtcgctggcg gcgggcggca gcaacctggt
     5941 gggcgacatc gaaggcgaca ccttctccag ccccgacggg ctgcgcatcg accccgaggg
     6001 ccgcatgtgg gtgcagaccg atcacagcgt tccgggcaat tcgggcctcg acggcacgac
     6061 gatcgaccaa gcctttggcc ataatgcgat gttctatgtc gatcagcaga acaagcagtc
     6121 gaaacgcttc ctcgtcgggc cgctcggctg cgagatcacg ggtctggcct atacgcccga
     6181 cctcacgacc ttcttcgtca acatccagca cccgaccggc gactggccgg tcgcgggcga
     6241 agcgccgcgg tcgtcgacga tcgtggtgcg gcggaccgac aaccagccgg tcggtaactg
     6301 atcgcgccgg ggccggtcgc gggcggcc
//
LOCUS       DATMSU010000194         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_136002,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000194 DATMSU010000000
VERSION     DATMSU010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136002"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VN034_04960"
     CDS             <1..860
                     /locus_tag="VN034_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWV59978.1"
                     /translation="RGRVGDSLYRSARAAGAPPQAIQSYLRVIGQQISVGSGIRSNDE
                     FDIIVDYRRAETGESETGKLLYAGLIRGGKPKVSMLEWNADGRSQWFEASGVGEQRGG
                     MTRPTRGRVTSNFGMRRHPILGYKRMHSGMDFGGGYGAPIYAVTDGVVTIAGRHGGFG
                     NYVKLNHGKGLGTGYGHMSRIAVRSGQRVKRGQVIGYIGSTGLSTGPHLHYELYRNGR
                     AVNPSSVTFVTRALLEGKALAAFRARIRELTSVAPGAALTPIAPKQLEGPKLGSLADV
                     ASKRVGDGI"
     gene            1044..2042
                     /gene="hemB"
                     /locus_tag="VN034_04965"
     CDS             1044..2042
                     /gene="hemB"
                     /locus_tag="VN034_04965"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWV59979.1"
                     /translation="MTQAAFPDLRLRRTRRTGWSRAMVRETHLTPANLIWPLFVCDGS
                     GSEEPVASLPGVSRWSVDLLVERAKDAVAAGIPCLALFPYTQAERRSADGGEALNPDN
                     LMCRATAAIKQALGDDIGVLTDVALDPYTSHGQDGLIDDKGYVLNDETVEILVGQALG
                     QARAGADIIAPSDMMDGRIGAIRQALEAEGFGHVQIMSYAAKYASAFYGPFRDAVGSR
                     GLLKGDKKTYQMDPANSEEALREVAQDLAEGADSVMVKPGLPYLDIIRAVKDNFAVPV
                     YAYQVSGEYAMIEAAAAVGAGDRDALVLETLLAFRRAGASGVLSYHALHAARLLGA"
     gene            2047..>2613
                     /locus_tag="VN034_04970"
     CDS             2047..>2613
                     /locus_tag="VN034_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59980.1"
                     /translation="MTASSSPRRWLFVLTILVGSFLLFLVQPMVARMVLPKLGGAPAV
                     WNSAMLVYQALLLGGYAYAHWLGRFTVRRQAMIHLALFLAAALWLPIGIAEIAPPGPG
                     QEALWVPLLLLASIGPVFFVVSAQAPLMQRWFAADARAGDPYYLYAASNLGSFAGLIS
                     YPALVEPAMPLAAQSWGWTAGYALLVLLV"
BASE COUNT          391 a          905 c          881 g          436 t
ORIGIN      
        1 tccgcggccg cgtcggcgac agcctgtacc gctcggcgcg tgccgccggt gcgccgccgc
       61 aggcgatcca gtcgtacctg cgcgtcatcg gccagcagat ttcggtcggc agtggtatcc
      121 gttcgaacga cgaattcgac atcatcgtcg attatcgccg cgccgaaacg ggcgagagcg
      181 agacgggcaa gctgctctac gccgggctga tccgcggcgg gaaacccaaa gtctcgatgc
      241 tcgaatggaa tgccgacggc cgctcccaat ggttcgaggc gtcgggcgtc ggcgaacagc
      301 gcggcggcat gacgcggccg acgcgcggac gcgtcacctc gaacttcggg atgcggcgcc
      361 acccgatcct cggttacaag cgcatgcaca gcggcatgga tttcggcggc ggctatggcg
      421 cgccgatcta tgcggtgacc gacggcgtgg tgacgatcgc cgggcgccac ggcggtttcg
      481 gcaattatgt gaagctcaat cacggcaagg gcctcggcac cggttacggc catatgagcc
      541 gcatcgccgt acgatcgggc cagcgcgtca agcgcgggca ggtgatcggc tatatcggct
      601 cgaccggcct ctcgaccggc ccgcatctcc attacgagct gtatcgcaac ggccgtgcgg
      661 tcaatccgtc gtcggtgacc ttcgtcaccc gcgcgctgct cgagggcaag gcgctcgccg
      721 ccttccgcgc gcgcatccgc gaattgacgt cggtcgctcc cggcgccgcg ctgaccccga
      781 tcgcgccgaa gcagctcgag ggacccaagc tcggcagcct cgccgatgtg gcgtcgaaaa
      841 gggtcggcga cgggatctga agcaatcacg ccctcccatt caggggaggg cagcgagact
      901 tgccagcttg ctggcttagt cgcagcgggt ggggccagcg ggtcccgtgc tacgccgcga
      961 gcccccaccc caaccctccc ctgaagggga ggggctttag aagaaccatt tgccccgcgc
     1021 ccgcgcgcgg ctatgcacgc cgcatgaccc aagccgcttt ccccgacctg cgcctgcgcc
     1081 gcacccgccg taccggctgg agccgcgcga tggtgcgcga aacgcatctg acgcccgcga
     1141 acctcatctg gccgctcttt gtctgcgacg gatcgggcag cgaggaaccg gtcgcgagcc
     1201 tgcccggcgt gtcgcgctgg tcggtcgatt tgctcgtcga acgcgcgaaa gacgcggtcg
     1261 ccgcgggcat cccgtgcctc gcgctctttc cctatacgca ggccgagcgg cgcagcgcgg
     1321 atggcggcga ggcgctcaat cccgataacc tcatgtgccg cgccaccgcc gcgatcaagc
     1381 aggcgctcgg cgacgacatc ggcgtgctca ccgacgtcgc gctcgatcct tacaccagcc
     1441 atgggcagga cgggctgatc gacgataagg gctatgtcct caacgacgag acggtcgaaa
     1501 tactcgtcgg gcaggcgctg ggtcaagcgc gcgcgggcgc cgacatcatc gcgccgagcg
     1561 acatgatgga cggccgcatc ggggcgatcc ggcaggcgct cgaggccgaa ggcttcggcc
     1621 atgtccagat catgagctat gccgccaaat atgcctcggc cttctatggc cccttccgcg
     1681 acgcggtcgg ctcgcgcggg ctgctcaagg gcgacaagaa gacctatcag atggaccccg
     1741 cgaacagcga ggaagccttg cgcgaggtcg cgcaggatct ggccgagggc gccgacagcg
     1801 tgatggtgaa gccggggctg ccctatctcg acatcatacg cgcggtgaag gacaatttcg
     1861 cggtgcccgt ctacgcctat caggtgtcgg gcgaatatgc gatgatcgag gcggcggcgg
     1921 cagtgggtgc gggcgaccgc gacgcgctcg tcctcgaaac cctgctcgcc ttccgccgcg
     1981 ccggcgcgtc gggcgtgctc agctatcacg cgctccacgc ggcgcggctg ctcggcgcct
     2041 gatagcatga ccgcttcttc atcgccgcgc cgctggctgt tcgtgctgac gatcctcgtc
     2101 ggcagcttcc tcctcttcct cgtccagccg atggtcgcgc ggatggtgct gcccaagctg
     2161 ggcggcgcgc ccgcggtgtg gaacagcgcg atgctcgtct atcaggcgct gctgctcggc
     2221 ggttacgcct atgcgcactg gctcggccgt ttcacggtgc ggcgacaggc gatgatccat
     2281 ctcgcgctgt tcctcgccgc cgcgctgtgg cttccgatcg gcatcgccga gatcgcgccg
     2341 cccggaccgg ggcaagaggc tttgtgggtg cccttgctcc tgctcgcgtc gatcggcccc
     2401 gtcttcttcg tcgtttcggc gcaggcgccg ctgatgcagc gctggttcgc cgccgacgcg
     2461 cgtgcgggcg acccctatta tctctacgcc gcctcgaacc tcggcagctt tgcgggcctg
     2521 atcagctatc cggcgctggt cgagccggcg atgccgctcg cggcgcaaag ctggggctgg
     2581 accgcgggct atgcgctgct cgttctgctc gtc
//
LOCUS       DATMSU010000195         2680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_136012,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000195 DATMSU010000000
VERSION     DATMSU010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136012"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VN034_04975"
     CDS             <1..600
                     /locus_tag="VN034_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HWV59981.1"
                     /translation="IESNENAARAYEVGREISVTHSPQGRLRRVSVAVALNQGKKALT
                     QADLTKIDSLVKGAIGYDAARGDLVAISQRPFAPVENTEPAFYDQGWFLPLVKQVGAI
                     LAALLAFLFIGRPMIRAAKERAARRAEQNQELEASLLAATDRPALASGSNHREITLEM
                     IEQAPSYEARANLVRAFVRQDSARAALVVRHLMQEGARA"
     gene            593..1624
                     /gene="fliG"
                     /locus_tag="VN034_04980"
     CDS             593..1624
                     /gene="fliG"
                     /locus_tag="VN034_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HWV59982.1"
                     /translation="MPEVAELDNMPALPAIEGSSAAAILLMLLDESEAATILKQLDPD
                     EVRQLAKAMFDTANASEQQIGQALDRFVTRSRDVSALAIGADTRIRTVINEAVGNVRA
                     DNILAAVAPQRSAASLEMLRWMDVDAISGLLASEHPQVGALILSVLVPDVAARAIETL
                     DEVLQADLVLRAAMLTAVPAAAIEDLESVLASANVDGQRVAKQAIGGPSDVAKIMKKM
                     PKQLSERTLRSLKKHDRILAQTIEEEMFIFENLRDLDKKSLSSVLRSVDAAQLAIALK
                     GADEDMVDMCLATMSQRAAETIRDEMDEMTMVKRADVDDAQKSVMQIVRQMAAAGEIM
                     IAGGGDDYV"
     gene            1617..2216
                     /locus_tag="VN034_04985"
     CDS             1617..2216
                     /locus_tag="VN034_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HWV59983.1"
                     /translation="MSDRAAETGFAPVRLADAMARGAGFRPLSFAPAQQAMPEQPESL
                     DEIDLDDPFALGLAEGQRLAEAAFVAERHQLLALLAGAEALQDEPSEELAQLIATTVE
                     RLVRQIVATAPIDAEWLQAQVETAAAMIADADKARTLWVHPDDAALLADCPLSMAVES
                     DAAMMRGTVRIETSTGWIEHGRAVYLEELRSALGESEAA"
     gene            2213..>2680
                     /gene="fliI"
                     /locus_tag="VN034_04990"
     CDS             2213..>2680
                     /gene="fliI"
                     /locus_tag="VN034_04990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543202.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HWV59984.1"
                     /translation="MTRRLALSAQQLLEPVDLAHASPRRIGTLVSHEGIMLEVSGFPM
                     PLGSNVRIRSADNDYVYGEVVGFRGHRSLVLPFDSGKPLVTGAPVEPHGASSMVPVGK
                     ALLGRIMDAQGNPLDGRPAIKSQFQWPLAGRKVNPLRRGRVTRALNMGVRAING"
BASE COUNT          461 a          957 c          867 g          395 t
ORIGIN      
        1 atcgaaagca acgaaaacgc cgcgcgcgcc tatgaggtcg gccgcgagat ttcggtgacg
       61 cattcgccgc agggcaggct gcgccgggtc tcggtcgccg tcgcgctcaa ccagggcaag
      121 aaggcgctga cgcaggccga cctgaccaag atcgacagcc tcgtcaaagg cgcgatcggc
      181 tatgatgccg cgcgcggcga cctcgtcgcg atcagccagc gccccttcgc ccccgtcgag
      241 aacaccgaac cggcctttta cgatcagggc tggttcctgc cgctcgtcaa gcaggtcggc
      301 gcgatcctcg ccgcgctgct cgccttcctg ttcatcggcc gcccgatgat ccgcgccgcc
      361 aaggaacgtg ccgcgaggcg cgccgaacag aatcaggagc tcgaagcatc gctgctcgcc
      421 gcgaccgacc gtccggcgct tgccagcggc tcgaaccatc gcgagatcac gctcgagatg
      481 atcgaacagg cgccgagcta tgaagcgcgc gccaatctcg tccgcgcttt cgtccggcag
      541 gattcggcgc gcgccgcgct cgtcgtccgc cacctgatgc aggagggcgc ccgtgcctga
      601 ggtcgccgaa ctggataata tgcccgcgct gccggcgatc gaaggatcgt ccgccgccgc
      661 gatcctgctc atgctgctcg acgaaagcga agccgcgacg atcctgaagc agctcgaccc
      721 cgacgaagtg cgccagctgg caaaggcgat gttcgacacc gccaacgcca gcgagcagca
      781 gatcgggcag gcgctcgatc gcttcgtgac gcgcagccgc gacgtcagcg cgctcgcgat
      841 cggcgccgac acgcgcatcc gcaccgtgat caacgaagcc gtcggcaacg tccgcgccga
      901 caatatcctc gccgccgtcg cgccgcagcg cagcgccgcg tcgctcgaaa tgctgcgctg
      961 gatggacgtc gatgcgatca gcggcctgct cgcgagcgag catccgcagg tcggcgcgct
     1021 gatcctttcg gtgctcgtcc ccgacgtcgc cgcgcgcgcg atcgagacgc tcgacgaagt
     1081 gctgcaggcc gatctggtgc tgcgcgcggc gatgctgacc gcggtccccg ccgcggcgat
     1141 cgaggatctc gaatcggtgc tcgccagcgc caatgtcgat ggccagcgcg tcgccaagca
     1201 ggcgatcggc ggcccgagcg acgtcgccaa gatcatgaag aagatgccga agcagctttc
     1261 ggaacgcacg ctccgttcgc tcaagaagca cgaccgcatc ctcgcgcaga cgatcgagga
     1321 agagatgttc atcttcgaaa atttgcgcga cctcgacaag aagagcctca gcagcgtgct
     1381 gcgctcggtc gacgccgccc agctcgcgat cgcgctcaag ggcgccgacg aggatatggt
     1441 cgacatgtgc cttgccacca tgtcgcagcg tgcggccgaa acgatccgcg acgagatgga
     1501 cgagatgacg atggtcaagc gcgccgacgt cgacgacgcg cagaagagcg tgatgcagat
     1561 cgtgcgccag atggccgcgg ccggcgagat catgatcgcg ggcggaggcg acgattatgt
     1621 ctgatcgcgc cgccgaaacc ggcttcgccc ccgtccgcct tgccgatgcg atggcgcgcg
     1681 gcgcgggctt tcgcccgcta tccttcgcgc ccgcccagca ggccatgccg gaacagcccg
     1741 aaagcctcga cgaaatcgac ctcgacgatc cctttgcgct cggccttgcc gaaggccagc
     1801 gtcttgccga agccgccttt gtcgccgagc gccaccagct gctcgcgctg ctcgccggcg
     1861 ccgaggcgct gcaggacgaa ccgagcgaag agctcgcgca gctgatcgcg acgacggtcg
     1921 aacgcctcgt gcgccagatc gtcgccaccg cgccgatcga cgccgaatgg ctgcaggcgc
     1981 aggtcgagac cgcggcggcg atgatcgccg acgccgacaa ggcgcgcact ttgtgggtcc
     2041 accccgacga cgccgcccta ctcgcggatt gcccgctttc gatggcggtc gagagcgacg
     2101 cggcgatgat gcgcggcacc gtgcgcatcg aaacctcgac cggctggatc gagcacggcc
     2161 gcgctgtcta tcttgaggaa ttgcgctcgg cgcttggcga aagcgaagcc gcatgacgcg
     2221 ccgcctcgcc ctttccgcgc agcagttgct cgagcccgtc gacctggcac atgcgagccc
     2281 gcgccgcatc ggcacgctcg tctcgcacga gggcatcatg ctcgaagtgt cgggtttccc
     2341 gatgccgctc ggcagcaatg tccgcatccg gtcggccgac aatgactatg tctatggcga
     2401 agtcgtcggc tttcgcggcc accgcagcct cgtccttccc ttcgactcgg gcaaaccgct
     2461 cgtcaccggt gccccggtcg aaccgcatgg cgcgtcgagc atggtccccg tcggcaaggc
     2521 tttgctcggc cgcatcatgg acgcgcaggg caacccgctc gacggccgcc cggcgatcaa
     2581 atcgcagttc cagtggccgc tcgccggccg caaggtcaat ccgctgcgcc gcggccgcgt
     2641 cacccgcgcg ctcaacatgg gcgtgcgcgc gatcaacggc
//
LOCUS       DATMSU010000196         5513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_69703, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000196 DATMSU010000000
VERSION     DATMSU010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69703"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VN034_04995"
     CDS             complement(<1..1254)
                     /locus_tag="VN034_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HWV59985.1"
                     /translation="MERLIGLLGIAALLAIAVLFSSNRRWIRLRVVIPAFLLQAGFAI
                     LVIGTPWGRAVIQAMSNAVSNLLGYAKAGTDFIFGPLASPEMGANSFAIAALPVIIFF
                     ASLISILYYLGIMQLVIKWVGGAIQKITGITKVESLGAAANIFVGQSESPLVIRPYLA
                     GLTPSQLFTVMTVGMAGVAGTILGAYASMIGEQLLPYLLAASFMSAPGGILMAKIMMP
                     DDPKDLGIEPVIMPEASHDEEKPANIIMAAAQGAQTGVKLAVAVGAMVLAFVALVALA
                     NGLLAGIGGWFGYPDLSFQAVIGAIFRPVMWLMGVPWEESAVVGGLFGSKVVLNEFVA
                     FIDLGRAQADMSLAAVAIATFALCGFANFSSIAIQMAVTGGLAPNQRPMIAKLGLKAL
                     LAGSLSNLMSAALAGLMLGIAPPLAT"
     gene            1421..3370
                     /locus_tag="VN034_05000"
     CDS             1421..3370
                     /locus_tag="VN034_05000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWV59986.1"
                     /translation="MRFVVAALFLAIFNSPSVSAEEKKGNADAAAFQPLPTSAFAQLP
                     FVEKADLSPDGTHMAGLFAIDGAQRIAMMPILGDRSKAVVVGIPDATEVSWIRWVGND
                     NIVVGLYALQSVQGDRWYISRLIGINRTTGKITKLLWEMSGQNAADLLWAPTDGSTEI
                     LVAAQNSIYEGSDFWPAVYRVDVATGRKRLEEKGRANILNWGADHLGRVRFGIGYRDN
                     TTKSTLLYRSGGDGLFRVIDSARLDEEEELTLPFMFIPDSDRGYVIKEIADGRQAVVE
                     VDIPTGRDVRTVYAAADTDVENPAVGPSGSRLLGVWTSDREQSLRWIDPAMAEHQKAL
                     DASSPQSMVHIDSWDGDLTSMLVRFRTPDNPGLLFHYDSTTGQLSRLAAINNAIGGKR
                     LSRAKLVRYKARDGLEIEGVLTMPRGRASKNLPFIVMPHGGPWGHDELSYDYWAQFLA
                     ERGYAVLQPNFRGSTGYGAEFEKAGQGQLGFAMQDDVTDGVRWAVSEGIADTKRVCIV
                     GGSYGGYAAMWGIAKDPDLYRCAISINGVANLRREVNDFGDHIRRRLYRSQWQKMTPD
                     FAAVSPINAIDRIKAPLLLIHGKKDVTVDHSQSARMYSAMKKADKTVEFVSVPLADHY
                     FTREADRMTLLRSIETFLRTHNPAD"
     gene            3413..4084
                     /locus_tag="VN034_05005"
     CDS             3413..4084
                     /locus_tag="VN034_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HWV59987.1"
                     /translation="MTEPTSAALPRTLSPSLFLFSIVYGGMVVLAGVLGNKQVALGSW
                     LAVEAGIFAFLLLVALSSAVSELHGQSVANRLVLWGFVPLVLSIMLTALVLALPASPE
                     MQPERLAAFDMILGQSPRLMAAGIVAYGTSQFLNVTIFSKLRGRDGAGTTWLAIRGAI
                     ASALSQIVDTLLFVTIAFYGVFPIANLMGGQMLAKVALSVLVIPFVITGLVALGRSLD
                     AKNAG"
     gene            4090..4989
                     /gene="argB"
                     /locus_tag="VN034_05010"
     CDS             4090..4989
                     /gene="argB"
                     /locus_tag="VN034_05010"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWV59988.1"
                     /translation="MTDPSKMPDLLAKAETLVEALPYLQRYAGETFVIKYGGHAMGDP
                     EAQRDFAEDVVLLKAVGINPVVVHGGGPQIGAMLKQLGIESTFVGGLRVTDAATAEVA
                     EMVLAGKINKEIVSWIAGLGGRAVGISGKDANLVLAEKVARTEPDPNSGIERHVDLGF
                     VGEPVAVDPTILVNLTNDNFIPIVAPVALGADGATYNINADTMAGAIAGALGAKRFFL
                     LTDVAGVLDKSGALLTDLDRTAIDGLKGDGTITGGMIPKVETCVAAVDAGVEAAVILD
                     GRIPHAMLLEIFTARGAGTLIHR"
     gene            5035..5334
                     /locus_tag="VN034_05015"
     CDS             5035..5334
                     /locus_tag="VN034_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWV59989.1"
                     /translation="MFLDILSILLNILWWIIIVQAVMSWLIAFNVINTHNDFVGQLWH
                     VLDRITEPLYRPFRRIMPDFGGIDLTPMVVLILLIIIQGPVMSYLYRLGVQTGMA"
     gene            5338..>5513
                     /locus_tag="VN034_05020"
     CDS             5338..>5513
                     /locus_tag="VN034_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HWV59990.1"
                     /translation="MAGSVPAAKVIDGKAFAAGLRARIADAVPAFRAAAGRAPGLAVV
                     LVGDDPASAVYVGS"
BASE COUNT          997 a         1761 c         1739 g         1016 t
ORIGIN      
        1 ggtggccagc ggcggcgcga tccccagcat cagccccgcg agcgccgccg acatcaggtt
       61 cgacaggctg cccgcgagca gcgccttcag cccgagcttg gcgatcatcg gacgctggtt
      121 cggcgcgagc ccgccggtca ccgccatctg gatcgcgatc gagctgaaat tggcgaagcc
      181 gcacagagca aaggtcgcga tcgcgaccgc cgccagcgac atgtcggcct gcgccctgcc
      241 gaggtcgatg aaggccacga attcgttgag cacgaccttg ctgccgaaca ggccgccgac
      301 gaccgcgctt tcctcccacg gcacgcccat cagccacatc accgggcgga agatcgcgcc
      361 gatgaccgcc tggaacgaca ggtccgggta gccgaaccag ccgccgatcc cggcgagcag
      421 cccgttggcg agcgcgacga gcgcgacgaa ggcgagcacc atcgcgccga ccgcgaccgc
      481 cagcttgacc cccgtctgcg cgccctgcgc cgccgccatg atgatgttgg cgggcttttc
      541 ctcgtcgtgg ctcgcctcgg gcatgatcac gggttcgatg ccgaggtcct tgggatcgtc
      601 gggcatcatg atcttggcca tcaatatgcc ccccggcgcg gacatgaagc tcgccgcgag
      661 cagatagggc aggagctgct cgccgatcat gctcgcatag gcgccgagga tcgtgcccgc
      721 gacgcccgcc atgccgaccg tcatcaccgt gaagagctgc gaaggggtca gcccggcgag
      781 ataggggcgg atgacgagcg gcgattcgga ttggccgacg aagatgttcg ccgccgcgcc
      841 caggctttcg accttggtga tacccgtgat cttctgaatc gccccgccga cccatttgat
      901 cacgagctgc atgatgccga gataatagag aatcgagatg agcgaggcga agaagatgat
      961 gacgggcagc gcggcgatcg cgaagctgtt ggcccccatt tcgggcgagg ccagcgggcc
     1021 gaagatgaaa tcggtgcccg ccttggcata gccgagcagg ttcgagacgg cgttcgacat
     1081 cgcctggatc accgcgcgac cccacggcgt cccgatgacg aggatcgcga acccggcctg
     1141 gagcaggaaa gcgggaatga cgacgcgcag ccgaatccat cgccggttcg acgaaaaaag
     1201 cacggcaatc gcaagcaatg cggcgatgcc gagcagaccg atgaggcgtt ccatattcct
     1261 tgtgcgtccc ccgcaagatt atcccgcctg tctagcgggc tttccctgcc gcacaagaaa
     1321 aaggagagac ctccggcgcg gcaagaaaat gccgcatcct cttggctcgg aggaattttc
     1381 tttataatca tcgccggaaa tcttcggggg gagatacgag gtgcgttttg tcgttgcggc
     1441 gctttttcta gccattttca acagtccgtc ggtttcggcg gaagaaaaga agggtaatgc
     1501 tgacgccgcg gccttccagc cgcttccgac cagcgccttc gcccagcttc cctttgtcga
     1561 aaaggccgac ctctcgcccg acggaacgca tatggccggc ctcttcgcga tcgacggcgc
     1621 gcaacgcatc gcgatgatgc cgatcctcgg cgatcgcagc aaggcggtgg tggtggggat
     1681 tcccgatgca accgaagtgt cgtggattcg ctgggtaggc aacgacaata tcgtcgtcgg
     1741 gctctatgcc ttgcagtcgg tccaaggcga ccgctggtat atttcccgcc tgatcggcat
     1801 caatcgcacg accggaaaga tcaccaagct gctctgggag atgagcggtc aaaatgcggc
     1861 cgatctgctc tgggcgccga ccgatggcag caccgaaatc ctggtcgcgg cgcaaaattc
     1921 catttatgaa ggctccgatt tctggcctgc ggtctatcgc gtcgatgtgg cgacgggacg
     1981 gaagcgcctt gaggagaagg gacgggccaa catcctcaac tggggcgccg accatctcgg
     2041 ccgcgtccgg ttcggcatcg ggtatcgcga caacaccacc aagtcgacat tgctctatcg
     2101 ctcgggcggc gacggccttt ttcgcgtcat cgacagcgcc cgtctggacg aagaggaaga
     2161 gttgacgctt cccttcatgt tcattcccga cagcgatcgt ggctatgtga tcaaggaaat
     2221 cgcggatggt cgccaggcag tcgtcgaagt cgacatcccg accggccgcg atgtgcgaac
     2281 cgtctacgcc gctgccgata ccgatgtgga aaatccggca gtcggcccca gcggatccag
     2341 gctgctgggc gtctggacca gcgaccgcga acagtcgctt cgctggatcg atccggcgat
     2401 ggccgagcat caaaaggcgc tcgacgcctc atcgccccag tcgatggttc atatcgacag
     2461 ttgggacggt gatttgacga gcatgctggt gcggttccgt acccccgaca atccggggct
     2521 gttgttccat tacgattcga caacaggcca gctttcccga ctagcggcga taaataatgc
     2581 gataggcggc aagcgcctgt cgcgggcgaa gctggtccgg tacaaggcgc gcgacggact
     2641 cgagatcgaa ggcgtgctga ccatgccgcg cgggcgggct tcgaaaaacc ttcccttcat
     2701 cgtcatgccg cacggcgggc cgtgggggca tgacgaatta tcctatgatt attgggccca
     2761 gttccttgcc gaacgtggtt atgcggtgct ccagccgaac ttccgcggct cgaccggcta
     2821 tggtgccgaa ttcgagaagg cggggcaggg gcaactcggc tttgcgatgc aggacgacgt
     2881 caccgacggc gttcgctggg ctgtcagcga gggcatcgcc gataccaaac gcgtctgcat
     2941 cgtgggcggc tcctatggcg gctatgccgc gatgtggggg atcgccaagg atcccgacct
     3001 gtatcgctgc gcgatttcga tcaacggcgt cgccaacctg cgtcgcgagg tcaatgattt
     3061 cggcgaccac atacgtcgcc gcctctaccg cagccagtgg cagaaaatga cgccggactt
     3121 cgccgcggtc tcgccgatca atgccatcga tcgcatcaag gctcccttgc tgctgattca
     3181 cggcaagaag gacgtgaccg tcgaccatag ccagtcggcg cgtatgtatt cggcgatgaa
     3241 gaaggcggac aaaaccgtgg agtttgtttc ggtaccgctc gccgaccatt atttcacgcg
     3301 cgaggccgat cgaatgacct tgctgaggtc gatcgagact ttccttcgga cgcataatcc
     3361 ggccgattga ccgccatctt tccactgagg gcaaaagcga ctatcgggcg gcatgaccga
     3421 acccacctcg gcggcgttgc cgcgcacgct ctctccgtcg ctctttctct tctcgattgt
     3481 ctatggcggc atggtcgtgc tcgccggcgt gctcgggaac aagcaggtcg cgctgggaag
     3541 ctggctcgcg gtcgaggccg gaatcttcgc ctttctgctg ctcgtcgcgt tgtcgagcgc
     3601 ggtgtccgaa ctccatgggc agtcggtcgc gaaccggctg gtgctttggg gcttcgtccc
     3661 gctcgtcctg tcgatcatgc tgaccgcgct cgtcctcgcg ctgcccgcat cgcccgaaat
     3721 gcagcccgaa cggctcgcgg ccttcgatat gatcctcggc cagagcccgc gcctgatggc
     3781 ggcgggcatc gtcgcttatg ggacctcgca attcctcaac gtcacgatct tcagcaagct
     3841 tcgcggacgc gacggagcgg gcacgacctg gctcgcgatc cgcggcgcga tcgccagcgc
     3901 gctgagccag atcgtcgaca cgttgctgtt cgtcacgatc gccttttacg gcgtgttccc
     3961 gatcgcaaac ctgatgggcg ggcagatgct cgccaaggtc gcgctgtcgg tgctcgtcat
     4021 tcctttcgtc atcactgggc tggtcgcact cggccgcagc ctcgatgcga agaacgccgg
     4081 atgatatcga tgaccgaccc ctcgaaaatg cccgacctcc tcgccaaagc cgaaacgctc
     4141 gtcgaggcgc tgccctatct gcagcgttat gccggcgaga ctttcgtgat caaatatggc
     4201 ggccacgcga tgggcgatcc ggaggcgcag cgcgacttcg ccgaggatgt cgtgctgttg
     4261 aaggcggtcg gcatcaaccc cgtggtcgtg cacggcggcg ggccgcagat cggcgcgatg
     4321 ctgaaacagc tcgggatcga atcgaccttc gtcggcgggc ttcgcgtcac cgacgccgcg
     4381 accgccgaag tcgccgagat ggtgctcgcg ggcaagatca acaaggaaat cgtcagctgg
     4441 atcgccgggc tcggcggccg cgcggtcggc atttcgggca aagacgcgaa cctcgtcctt
     4501 gccgaaaagg tcgcgcgcac cgagcccgat cccaattcgg gcatcgagcg ccacgtcgac
     4561 ctgggcttcg tcggcgagcc cgtcgcggtc gacccgacga tcctcgtcaa cctgaccaac
     4621 gacaatttca tcccgatcgt cgcgcctgtc gcgctgggcg ccgacggcgc gacctataat
     4681 atcaacgccg acacgatggc gggtgcgatc gcgggcgcgc tcggcgcaaa gcgtttcttc
     4741 ctgctcaccg acgtcgccgg ggtgctcgac aagtcgggcg cgctgctcac cgatctcgac
     4801 cgcaccgcga tcgacgggct gaagggcgac ggaacgatca ccggaggcat gatccccaag
     4861 gtcgaaacct gcgtcgcggc ggtcgatgcg ggggtcgagg cggcggtcat cctcgacggc
     4921 cgcatcccgc acgcgatgct gctcgaaatt ttcaccgcaa ggggtgcggg cacgctcatt
     4981 caccgctaag accaagctat cgacctttcg gccggagacc ttgccttgta tttcatgttt
     5041 ctcgatattc tctcgatcct gctcaacatc ctctggtgga tcatcatcgt tcaggcggtg
     5101 atgtcgtggc tgatcgcgtt caacgtcatc aacacgcaca atgatttcgt cggacagctc
     5161 tggcatgtgc tcgaccggat caccgagccg ctctatcgcc cattccgccg catcatgccc
     5221 gatttcgggg gcatcgacct gacgccgatg gtggtgctga tcctgctcat catcatccag
     5281 ggcccggtga tgtcctatct ctaccggctc ggcgtccaga ccgggatggc ctgaggcatg
     5341 gccggttcgg ttcccgcggc caaagtgatc gacggcaagg ctttcgctgc tgggcttcgc
     5401 gcgcggatcg ccgatgcggt tccggccttt cgtgcggcgg cgggacgcgc gcccgggctc
     5461 gccgtggttc tcgtcggcga cgatccggcg agcgcggtct acgtcgggtc gaa
//
LOCUS       DATMSU010000197         5083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_174152,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000197 DATMSU010000000
VERSION     DATMSU010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174152"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VN034_05025"
     CDS             complement(<1..297)
                     /locus_tag="VN034_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV59991.1"
                     /translation="MSLLPAFRRPGILLLLPLLLALAARVLVAPGWMIESDAGGSITV
                     RICSDPANPGGTMTIPIEKTGDHNAGEAQQHCPWGALANAPIVPDLPVLAAAPMV"
     gene            complement(362..1531)
                     /locus_tag="VN034_05030"
     CDS             complement(362..1531)
                     /locus_tag="VN034_05030"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWV59992.1"
                     /translation="MAKRDYLQNLMRDLESHTEVRRFGSGWLSGFFGLLFAIAGFFMV
                     IALRFPDWFATPELEIIKHWGGFRGLVHATLLISYGLSLLSLLLRPRKVLGLTALMIG
                     LAAILVGGANVQPQETRDWGIFFGLDFFVVNLLVTGFMFAPLERAFPHRRTQRLFRTE
                     WREDLFYYLVSTMFVQVLGFLALAPSTIINANTSSWEAFRATVASLPWIVQFVIVLVA
                     SDLAQYFFHRSFHRYPFLWGFHAVHHSAKSMDWLAGSRMHFVEIILLRSITSLPLFTL
                     GFAPSVMQAYIGFVYVWSSLLHANVGGHFNRLGHWIATPRFHHWHHGLEREAFDVNFA
                     IHFPWIDKLFGTFHLPKDRWPENYGIPEDVPKDYWGQFLYPWTRTGKKTDETPAE"
     gene            complement(1692..3866)
                     /gene="ligA"
                     /locus_tag="VN034_05035"
     CDS             complement(1692..3866)
                     /gene="ligA"
                     /locus_tag="VN034_05035"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWV59993.1"
                     /translation="MTEIESLSEAAAANELMRLAKQIAYHSKRYHAEDAPEISDADYD
                     ALVRRNNELENAFPQLIRADSPNNQVGAAVEGSPLAKVAHAVRMMSLDNAFVAEDVAE
                     FAARVRRFLNLGADAAVALTAEDKIDGLSCSLRYEKGKLVQAATRGDGSVGEDVTANV
                     RHIADIPQELKGDAPDVFEIRGEVYMAKTDFVALNARLMEEGEALAAQREQDFDPATV
                     RQFANPRNAAAGSLRQKDASVTASRPLRFLAHGWGEVSALPADTQFGVMKAVAGWGVP
                     VSPLLKRYDSVDAVLAHYAEIERRRAEMDYDIDGVVYKVDRLDWQARLGFVAKAPRWA
                     IAHKFPAERAQTTLEAIDIQVGRTGKLTPVGRLTPVTVGGVVVSNVTLHNRDEIGRLG
                     VRPGDRVVIQRAGDVIPQVVENLTRDEDRAAFAFPDHCPVCDSEAVAEEGEVDVRCTG
                     GLICNAQKFERLRHFVSRGALDIEGLGEKSIQEFLDLGWLDKGPADIFRLKAHRAELL
                     GREGWKEKSVDNLFAAIEAKRAPDAARLLFGLGIRHVGAVTARDLLKGLGDIRRLPEK
                     AAEIHAYLEANPQGEGESDGKYTARRVETFKAILEVRADGIGIAVGEALADFFHEPHN
                     RTLWDDLLSEVAPPLYVVETRESEVSGMTVVFTGKLETMSRDEAKAQAEALGAKAAGS
                     VSAKTDLVVAGPGAGSKLKQASALGIRVIDEAEWAEIVKAAG"
     gene            complement(3869..4267)
                     /locus_tag="VN034_05040"
     CDS             complement(3869..4267)
                     /locus_tag="VN034_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HWV59994.1"
                     /translation="MSRTKRHETLLSYAHKMRRQPTEAEKRLWRYLSGAKLNGHKFRR
                     QAILGPYIADFYCPQKNLTVEIDGDTHILEADATRDEALRNQGIEVVRVTNEDVLTNM
                     DGVLQYLLVKLEGRVNRWPHPNPSPEGEGL"
     gene            complement(4304..4696)
                     /locus_tag="VN034_05045"
     CDS             complement(4304..4696)
                     /locus_tag="VN034_05045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWV59995.1"
                     /translation="MNRREHLAAMLAMAAALLPTTKLRALEAKMEEADPQYGLLGQMM
                     AVPGKRSELVAILTEATGEMPGNIAYLIGEDSANPDAIWIVELWNSKEAHAASLKLPA
                     VQAAIAKGRPLIAGFGTRAEFKPVGIRN"
     gene            complement(4693..>5083)
                     /locus_tag="VN034_05050"
     CDS             complement(4693..>5083)
                     /locus_tag="VN034_05050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV59996.1"
                     /translation="GEPAILALGEADAVEMAALADHAKPGPWGPATHRYGPFFGIREE
                     GRLLAMAGQRILVPGMAEVSGVATWADCRGRGLARALIGHVMREMVVRGETPFLHSYA
                     DNAGAIGLYESLGFRIRREVHVLVIAK"
BASE COUNT          877 a         1647 c         1659 g          900 t
ORIGIN      
        1 gaccatgggt gctgcggcga gaaccggcag gtcgggaacg atcggcgcat tggcgagcgc
       61 gccccacggg caatgctgct gcgcttcgcc ggcattgtga tcgccggtct tttcgatcgg
      121 tatcgtcatc gtgccgccgg gattggcggg gtccgaacag atgcgcacgg tgatcgaccc
      181 gccggcatcg ctctcgatca tccagcccgg cgcgacgagc acccgcgcag caagcgcaag
      241 gagcagcggc aggagcagca ggatgccggg acggcgaaag gcggggagca ggctcacgct
      301 gcgcgcctta tggcgcgcgc gcgatgctgg caagcggaca gattgcccta ctccgccccc
      361 gctattcggc gggcgtctcg tcggtcttct tgcccgtgcg cgtccaagga tagaggaact
      421 ggccccaata atctttgggc acatcctcag ggatgccgta attttcgggc caacggtcct
      481 tgggcagatg gaaggttccg aagagcttgt cgatccacgg gaaatggatc gcgaaattga
      541 cgtcgaacgc ctcgcgctcg agcccgtgat gccagtggtg gaagcgcggc gtcgcgatcc
      601 agtggccgag ccggttgaaa tggccgccga cattggcgtg gagcagcgac gaccagacat
      661 agacgaagcc gatataggcc tgcatcaccg agggcgcgaa gccgagcgtg aacagcggca
      721 gcgaggtgat cgagcgcaag aggatgatct cgacgaaatg catccgcgat cccgcgagcc
      781 agtccatcga tttcgcgctg tggtgcaccg cgtggaaacc ccagaggaag gggtagcggt
      841 ggaagctgcg gtggaagaaa tattgcgcca gatccgacgc gacgagcacg atgacgaact
      901 ggacgatcca cggcagcgac gcgacggtgg cgcggaacgc ctcccagctc gacgtattcg
      961 cgttgatgat cgtcgaggga gcgagcgcga ggaagcccag cacctgcacg aacatcgtgc
     1021 tgacgagata atagaaaaga tcctcgcgcc attcggtgcg gaacagccgc tgggtgcggc
     1081 ggtgcgggaa agcgcgttcg agcggcgcga acatgaagcc ggtgacgagc aggttcacga
     1141 cgaagaagtc gagcccgaag aaaatgcccc agtcgcgcgt ctcctgcggc tggacattcg
     1201 cgccgccgac gaggatcgcg gcgagcccga tcatcagcgc ggtgaggccg agcacttttc
     1261 ggggccgcag cagcaggctg agcaaggaca ggccgtagct gatcagaagc gtcgcgtgga
     1321 cgaggccacg gaacccgccc caatgcttga taatctccag ctcgggggtc gcgaaccaat
     1381 cggggaagcg cagcgcgatc accatgaaga agcccgcgat cgcgaacagc agcccgaaaa
     1441 aacccgacag ccagccgctg ccgaaccgcc gcacctcggt atgcgattcg aggtcgcgca
     1501 tcagattttg cagataatca cgctttgcca tgtcgcccct tcacagcatt ttcgccgcgc
     1561 cgtctgtgac gacgggcacg gcttgctatc accttaatct tatcttcgtc atgccggact
     1621 tgatccggca tccattcctg ccccggcgtc atggaccccg gatcaagtcc ggggtgacga
     1681 aatgctattg ctcatcccgc cgcctttaca atctccgccc attcggcctc gtcgatcacg
     1741 cggatgccca gcgcgctcgc ctgcttgagc ttcgaccccg cgccggggcc cgcgacgacg
     1801 aggtcggtct tcgcgctcac gcttcccgcg gccttggcgc cgagcgcctc ggcctgcgct
     1861 ttggcttcgt cgcggctcat cgtctctaat ttgccggtga agacgacggt catccccgac
     1921 acctcgctct cgcgcgtctc gacgacataa agtggcggcg cgacttcgga gaggaggtcg
     1981 tcccacaggg tgcggttgtg cggttcgtgg aagaaatcgg cgagggcttc gccgaccgcg
     2041 ataccgatgc cgtcggcgcg cacttcgagg atcgccttga acgtctcgac ccggcgcgcc
     2101 gtatatttgc cgtcggactc gccctcccct tgcgggttgg cttcgagata agcgtgtatc
     2161 tcggcagcct tctcgggaag ccgccttatg tcgccgagcc ccttgagtag atcgcgcgcg
     2221 gtcaccgcgc cgacgtggcg gatgccgagc ccgaacagca ggcgcgcggc gtcgggcgcg
     2281 cgcttcgcct cgatcgcggc gaagagattg tcgaccgact tttccttcca cccctcgcgc
     2341 ccgagcagtt cggcgcggtg cgccttgaga cggaagatgt cggcgggacc cttgtcgagc
     2401 cagccgaggt cgagaaattc ctggatgctc ttttcaccca gcccctcgat gtcgagcgcg
     2461 ccgcggctga cgaagtggcg cagccgctcg aacttctgcg cgttgcagat gaggccgccg
     2521 gtgcagcgga catcgacctc gccctcctcg gcgaccgctt cgctgtcgca gacggggcaa
     2581 tgatcgggga aggcgaacgc cgcgcggtcc tcgtcgcgcg tgagattctc gacgacctgc
     2641 gggatcacat cgcccgcgcg ctggatgacg acgcggtcgc cgggacgcac gccgagccgg
     2701 ccgatctcgt cgcggttgtg aagcgtgacg ttcgagacga cgacgccgcc gacggtgacg
     2761 ggggtgagcc ggcccacggg ggtcagcttg ccggtgcggc cgacctgaat gtcgatcgct
     2821 tccaaagtcg tctgcgcgcg ctcggcgggg aatttatgcg cgatggccca gcgcggcgcc
     2881 ttggcgacga agcccagccg cgcttgccag tcgagccggt cgaccttgta gacgacgccg
     2941 tcgatatcat agtccatctc ggcgcggcgg cgctcgatct cggcataatg ggcaaggacg
     3001 gcgtcgacgc tgtcgtagcg tttgagcagc ggcgagaccg gaacgcccca gcccgcgacc
     3061 gccttcatca cgccgaactg ggtgtcggcg ggaagcgcgc tgacctcgcc ccagccgtgc
     3121 gcgaggaagc gcagcggacg cgatgcggtg acgcttgcgt ccttctggcg cagcgaaccc
     3181 gccgcggcgt tgcgcgggtt ggcgaactgg cggaccgtgg caggatcgaa atcctgttcg
     3241 cgctgcgccg cgagcgcctc gccctcctcc atcaaccgcg cgttgagcgc cacaaagtcg
     3301 gtcttcgcca tatagacctc gccgcgaatc tcgaagacgt cgggggcgtc gcccttcagc
     3361 tcctgcggta tgtcggcgat gtggcggacg ttggcggtaa catcctcgcc gacgctgccg
     3421 tcgccgcgcg tcgcggcctg gacgagcttg cccttttcat agcgcagcga acaggaaaga
     3481 ccgtcgatct tgtcttcggc ggtcagcgcg accgcggcat cggcgccgag gttgaggaag
     3541 cgccgcacgc gcgcggcgaa ctccgcgaca tcctcggcga caaaggcgtt gtcgaggctc
     3601 atcatccgca ccgcatgcgc gaccttcgcg agcggcgatc cctcgaccgc ggcgccgacc
     3661 tgattattcg ggctgtcggc gcggatcagc tgcggaaagg cgttctccag ctcgttgttg
     3721 cggcggacga gcgcgtcata gtcggcgtcc gaaatctcgg gcgcatcctc ggcatgataa
     3781 cgcttgctgt gataggcgat ctgcttcgcg aggcgcatca gttcattggc ggccgcggcc
     3841 tcggacagcg attcgatttc ggtcatgtct aaagcccctc cccttcaggg gaggggttgg
     3901 ggtggggcca tcggttgacg cggccttcga gttttacaag cagatattgc agcaccccgt
     3961 ccatgttcgt cagcacatcc tcgttagtca cacgaaccac ttcaatgcct tggttccgta
     4021 aggcctcgtc tcttgtagca tctgcctcaa gaatgtgagt atcgccgtct atctccacgg
     4081 tcagattttt ttgtgggcaa taaaaatcgg cgatgtaggg accgaggatc gcctgacgtc
     4141 gaaacttatg gccgttcagc ttggcacccg aaagatagcg ccacaatcgc ttctccgctt
     4201 ccgttggttg gcggcgcatc ttgtgcgcat agctcagcag agtttcgtgg cgttttgtac
     4261 gactcatagc cccaccccaa cccctcccct gaaggggagg ggcttaattc cttattccaa
     4321 ccggcttgaa ctccgcgcga gtgccaaatc cggcgatcag cgggcgcccc tttgcgatcg
     4381 ccgcctgcac cgcggggagc ttcagcgagg cggcgtgcgc ttccttgctg ttccacagtt
     4441 cgacgatcca gatcgcgtcg gggttcgcgc tgtcctcgcc gatcagatag gcgatattgc
     4501 cgggcatttc gcccgtcgct tcggtcagaa tcgcgaccag ttcggagcgc tttcccggca
     4561 cggccatcat ttggccgagc aggccatatt gcgggtcggc ttcttccatc ttcgcctcca
     4621 gagcacggag ttttgtcgtg gggagcagcg ccgcggccat cgcgagcatc gcggcgagat
     4681 gctcgcgccg gttcatttcg cgatcaccag cacatgcacc tcgcgccgga tgcggaaacc
     4741 gagcgattca tagaggccga tcgcccccgc attgtcggca tagctgtgca ggaagggcgt
     4801 ctcgccgcgc acgaccattt cgcgcatgac atggccgatc agcgcccgcg cgaggccgcg
     4861 cccgcggcaa tcggcccatg tcgcgacgcc gctgacctcg gccataccgg ggacgagaat
     4921 gcgctgcccc gccatcgcga gcagccgccc ttcctcgcgg atgccgaaga aagggccgta
     4981 gcggtgcgtt gccggccccc atggtccggg cttggcatgg tcggcgagcg ccgccatttc
     5041 gaccgcgtcg gcctcgccga gcgcgaggat cgccggttcg ccc
//
LOCUS       DATMSU010000198         3852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_136070,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000198 DATMSU010000000
VERSION     DATMSU010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136070"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..857)
                     /locus_tag="VN034_05055"
     CDS             complement(63..857)
                     /locus_tag="VN034_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV59997.1"
                     /translation="MTLGEEIRGHLPYLRRYARALTGSQQHGDNFVHTTLEVIVAAPD
                     EFHSGDGTRIDLYRNFHRIWESAYIDDGEGRDDEEHPLVRAAHQRLVQLTPLGRQILL
                     LTALEGFSVEEAGLITGTDQSTVEVLLADAVGELDRESRTSVLIIEDEPLIAMELEQI
                     VRDLGHRVAGIATTHEDAVAAFEKTDAGLVLADIQLADGSSGIDAVQDILAIAPVPAI
                     FITAFPERLLTGGRVEPTFLISKPFRENTVRAAISQSLLFTPQLAA"
     gene            1132..2607
                     /locus_tag="VN034_05060"
     CDS             1132..2607
                     /locus_tag="VN034_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWV59998.1"
                     /translation="MSDGGSEEATRAERLRLDTLREYRIMDTPPEAAFDRVTKMVADL
                     YGVPIALVSLLDECRQWFKSSHGLDATETPREISFCQYVIAEEQPVVVTDVLSDPRFR
                     DNPLVTGELHIRFYAGVPLRAYNGAILGTLCLIDREERAPLTATELERLEDFAGIIMA
                     EADLRRIGVERDEARRMLERALDFSGIATWQLHPRTGEMSWHGAAAELFGADYESGLI
                     TADDLLARMPPEDRERVTAALEESRDGGHPYSVEHRLQHPERGMRWIASRGDWDVWLD
                     DARLTGVSIDITEQKSRQENANLLMRELHHRMRNLFATVSAIISLTRHAARDVDDYVE
                     RISSRLDALNRAQNVLLGANFMTGSMHALMREVEAAFPRIRWSGPDLLLPENALVAMA
                     LFFNELATNAAKHGALSGANGRVEVSWTQEAEGSDDRMFRLTWAESGGDREVAVPERT
                     SFGTLLMERSVKNNLGGTIERRWEPGGLVVDITLPARWREA"
     gene            2810..>3852
                     /locus_tag="VN034_05065"
     CDS             2810..>3852
                     /locus_tag="VN034_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HWV59999.1"
                     /translation="MSAKAILEKLAVLADAAKYDASCASSGTVKRDSTESGGIGSTEG
                     MGICHSYAPDGRCISLLKILLTNFCIYDCRFCINRASSNVERARFSPQEVVRLTLDFY
                     KRNYIEGLFLSSGIIRSEDYTMEQLVEVARILREEHRFAGYIHLKTIAGADPGLITLA
                     GLYADRLSTNVELPTEEGLSSFAPEKKPWAIRKTMASVRVGEADAIEAAKSRLIGKAT
                     PPRFAPAGQSTQMIVGADAARDDDILKTASNLYSGYQLRRVYYSAYSPIPDASSDLPP
                     VRPPLMREHRLYQADWLLRFYGFERAEIMEGASGGMLDLAIDPKLAWALMRREAFPVD
                     INRAPRELLLRVPG"
BASE COUNT          683 a         1200 c         1291 g          678 t
ORIGIN      
        1 caacagcgta tcctgaaatg ggaggccggg cgggggagcg ggccggtgcc gctctcgcaa
       61 atttaggccg caagctgcgg cgtgaagagc aggctctggc tgatcgcggc gcgcacggta
      121 ttttcgcgga acggcttgga gatcaggaag gtcggctcga cccgcccgcc ggtgagcagc
      181 cgttcgggaa aggcggtgat gaagatcgcc ggcacggggg cgatcgcgag gatgtcctgc
      241 accgcatcga tgcccgagga tccgtcggca agctgaatgt cggcaaggac gaggccggcg
      301 tcggttttct cgaacgccgc gaccgcgtcc tcatgcgtcg tcgcgatccc cgcgacgcgg
      361 tggccgaggt cgcggacgat ctgttcgagt tccatcgcga tcagcggctc gtcttcgatg
      421 atcagcaccg aggtgcgcga ttcgcggtcg agttcgccga ccgcgtcggc cagcagcact
      481 tcgaccgtcg actggtcggt gccggtgatc agcccggcct cctcgaccga gaacccttcg
      541 agcgcggtga gcagcaagat ctgccggccg agcggggtga gctggacgag gcgctggtgc
      601 gcggcgcgga ccagcggatg ctcttcatcg tcgcggcctt cgccatcgtc gatataggcg
      661 ctttcccaga tgcggtgaaa attgcggtag aggtcgatcc gcgtgccgtc gccgctgtgg
      721 aattcatccg gagcggcgac gatgacttcg agggtggtgt gaacgaaatt gtcgccatgc
      781 tgctggctgc ccgtcagcgc gcgcgcatag cggcgcagat agggcagatg gccccgaatt
      841 tcctcaccca atgtcatcaa aatacctccc tcattaagag cagtcatacg cgtgcgcgtg
      901 cgggtcggtt cccttggtgg acgccgaaaa agcggtgcgt cgggctgcaa cttgtcgcgc
      961 actgaagcgt agactcagcc gggacgccag aacgccgcga tgtaagtggg gggaatgtca
     1021 cactttctta ttgcgcggca aggatgcttt tgtagggacc atttcatggc agagcggcgg
     1081 cccggggaat atagcggtgg gtcggcatcg cacgacggcg ggggacggca agtgtcggat
     1141 gggggcagcg aagaggcgac acgggccgaa cggctacgcc tcgacacgct tcgcgaatat
     1201 cgcatcatgg acaccccgcc cgaggcggct ttcgatcgcg tgaccaagat ggtcgccgac
     1261 ctctatggcg tgccgatcgc gctggtatcg ctcctcgacg aatgccgcca gtggttcaag
     1321 tcgagccatg gcctcgacgc gacggaaacg ccgcgcgaga tcagcttctg ccaatatgtc
     1381 attgccgagg agcagcccgt cgtggtgacc gatgtgctga gcgatccgcg ctttcgcgac
     1441 aatccgctcg tcaccggcga gctccatatc cgtttctatg ccggggtgcc gttgcgcgcc
     1501 tataatggcg cgatcctcgg gacgctgtgc ctgatcgacc gcgaagaacg cgcgcccttg
     1561 accgccaccg aactcgaacg gctggaagat ttcgccggta tcatcatggc agaggccgac
     1621 ctgcgccgca tcggcgtcga gcgcgacgag gcgaggcgga tgctcgaacg cgcgctcgat
     1681 ttttcgggga tcgcgacgtg gcagctgcac ccgcggaccg gcgagatgtc ctggcacggc
     1741 gccgccgccg aattgttcgg cgccgattat gaaagcgggc tgatcaccgc cgacgatctg
     1801 ctggcgcgta tgccgcccga ggaccgcgaa cgggtgaccg ccgcgctcga ggaatcgcgc
     1861 gacgggggtc atccctattc ggtcgagcat cggctccagc atcccgagcg cgggatgcgc
     1921 tggatcgcct cgcgcggcga ctgggacgtc tggctcgacg atgcgcggct gacgggggtc
     1981 agcatcgaca tcaccgagca gaagagccgc caggagaacg ccaacctgct gatgcgcgaa
     2041 ttgcaccacc ggatgcgcaa cctgttcgcg acggtcagcg cgatcatctc gctcacccgc
     2101 catgccgcgc gcgatgtcga cgattatgtc gaacggatca gcagccggct cgacgcgctc
     2161 aaccgcgcgc aaaatgtgtt gctcggcgcc aatttcatga ccgggtcgat gcacgcgctg
     2221 atgcgcgagg tcgaggcggc ctttccgcgc atccgctggt cggggcccga ccttttgctt
     2281 cccgaaaacg cgctggtcgc gatggcgctc ttcttcaacg aactcgcgac taatgccgcg
     2341 aagcatggcg cgctgagcgg cgcgaacggc cgcgtcgagg taagctggac gcaggaggcc
     2401 gagggcagcg acgatcgcat gttccgtctg acctgggccg aaagcggcgg cgatcgcgaa
     2461 gtcgccgtgc ccgagcgcac tagcttcggc acgctgctca tggagcgcag cgtcaagaac
     2521 aatctcggcg ggacgatcga gcggcgctgg gaacccggcg ggctggtcgt cgacattacg
     2581 ctccccgcgc ggtggcgcga ggcgtaggac cggtcgagat ttagcccctg acggcctgca
     2641 aatggcggcc ttccgcgctt ccggtgctca cgtgcagaaa gcacgctgcg ctccgggtca
     2701 cggaaaacca ccattttcga ctcgccagga gctaaatctc gaccggtccc agacgcaaac
     2761 ttaacctgga ttatttgttc tctctatgtt cctgtgttag ccatggggca tgtccgccaa
     2821 agcgattctc gaaaagctgg ccgtgctcgc cgacgcggcg aaatatgacg cgtcatgcgc
     2881 ctcgtcgggg acggtgaagc gcgattcgac cgaaagcggg gggatcggat cgaccgaggg
     2941 catgggcata tgccacagct atgcgcccga cgggcggtgc atttcgctgc tcaagatatt
     3001 gctcaccaac ttctgcatct atgactgccg cttctgcatc aaccgcgcgt cgagcaatgt
     3061 cgagcgcgcg cggttcagcc cgcaggaggt cgtgcggctc acgctcgatt tctacaagcg
     3121 taactatatc gaggggctgt tcctctcctc gggcatcatc cgctccgaag attatacgat
     3181 ggagcagctc gtcgaggtcg cgcgcatctt gcgcgaagag catcgctttg ccggctatat
     3241 ccatttgaag acgatcgccg gcgccgaccc gggactgatc acgctcgcgg ggctttatgc
     3301 cgatcgcctc tcgaccaatg tcgagctgcc gaccgaagaa gggctgtcga gtttcgcgcc
     3361 cgaaaagaag ccgtgggcga tccgcaagac gatggcgtcg gtgcgcgtcg gcgaggccga
     3421 tgcgatcgag gcggcgaaga gccggctgat cggcaaggcg acgccgccgc gcttcgcgcc
     3481 cgcggggcaa tcgacgcaga tgatcgtcgg tgccgatgcc gcgcgcgacg acgatatatt
     3541 gaagaccgcg agcaacctct attcgggcta tcagctgcgc cgcgtctatt attcggcgta
     3601 tagcccgatc cccgatgcca gcagcgattt gccccccgtg cggccgccgc tgatgcgcga
     3661 gcatcggctc tatcaggccg actggctgtt gcgcttctat ggtttcgagc gcgccgagat
     3721 catggagggc gcgagcggcg gcatgctcga cctcgccata gatcccaagc tcgcctgggc
     3781 gctgatgcgg cgcgaggcct ttccggtcga catcaaccgc gcgccgcgcg aattgctgct
     3841 gcgcgtgccc gg
//
LOCUS       DATMSU010000199        14239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_164420,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000199 DATMSU010000000
VERSION     DATMSU010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 14239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14239
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164420"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VN034_05070"
     CDS             <1..835
                     /locus_tag="VN034_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV60000.1"
                     /translation="TAGGSEEVDFVEAIAMRLPLDVILELLGVPADDRDQMYLWSNAV
                     IGSEDPEYGNVDLPREAILEAQQAMFGYFARFIAARRAAPREDLVSLLVEARIDGEPL
                     GDREILGFCFLLAIAGNETTRNATSGAMLALIEHPEARAALTADPSIMVPAVEELLRW
                     VTPIVYMARTATCDVDIAGQTVREGEKVVLFYPSANRDESMFENPFTLDLTRKPNRHL
                     TFGFGRHSCLGNDLARIELRAVLTELLTRFPGLELAGPVSRLRSNFVGGIKHMPVRLA
                     P"
     gene            895..2118
                     /locus_tag="VN034_05075"
     CDS             895..2118
                     /locus_tag="VN034_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWV60001.1"
                     /translation="MADWDSLYASRMHGVIASDIRERMKLLDAGEIIHLGGGLPDPAI
                     FPYAALSAASAAIWSDPARARTALQYAPSEGHAPLRTWIAERMTKEGAPCTADNLMIT
                     NGSQQGLDFIAKLLLSPGDRAMVEVPSFIGALRAFDAYQPHYVGLPQDSARWDAEALG
                     GAKFCYAGPDFRNPTGTTMTLGERMALLDLAEATGMVLVEDGCYEALRYDGEPVPSLL
                     KLAIRRHGDIEKSPVLHTGTFSKTIAPSLRVGWIAGPAHVIRKLVLIKQASDLATSAL
                     NQMLLLDVVETALDDTIARARRVYRERRDSMLASLAEAMPPGVTFTRPEGGLYVWAEL
                     PEGMDATLFARRALAEHKVSIIAGGAFYPAGTEKIPGHRTSFRLSFSMIDAARAREGV
                     MRLGELARQMMPETV"
     gene            complement(2121..2477)
                     /locus_tag="VN034_05080"
     CDS             complement(2121..2477)
                     /locus_tag="VN034_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWV60002.1"
                     /translation="MGDIRRIAANPRMSGAVVHAGVVYLSGQIAIDHRGGAVADQVDE
                     VLARIDALLEEAGSDRSRLLTAAILLSDMAVMPVLNAAWDQWIVPGCAPTRTSIQTVL
                     ASPDYALEIVITAAAD"
     gene            complement(2470..3390)
                     /locus_tag="VN034_05085"
     CDS             complement(2470..3390)
                     /locus_tag="VN034_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60003.1"
                     /translation="MRIDLSGRVAIVTGAGGGLGREHALLLAKRGAKVVVNDLGGARD
                     GSGGNATMAEQVVEEIRQAGGEAIANGASVNDFAEVEAMVGEVMGKWGRIDILVNNAG
                     ILRDRTFAKQDLSDFFTVIDVHLAGSVHCTKAVWGIMTAQGHGRIMMTTSSSGLYGNF
                     GQAQYGAAKLGLVGFMKTLAQEGRKHGIRVNALAPSAATRMTVDIMTEDDLAALSPAS
                     VAPGMLALVCDDAPTGMIMCAGAGSFEAAHITLTRGIAVEPGDDAAEHVLAGLDHILD
                     RTDETVPETGMQQSQYELSQRAKPLVTVDG"
     gene            3534..4448
                     /locus_tag="VN034_05090"
     CDS             3534..4448
                     /locus_tag="VN034_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV60004.1"
                     /translation="MSLDPEMRAILDRRKALNLPGFSAGTPVDARRSFAESQAALPPG
                     RGARNVTISNEAITGPKGDVPIRRYHPEGEAHGRILYLHGGGWVFGTLDGFDPVCRQL
                     ARESGAEIVSVDYRLAPEHPFPQPLDDCWAALLALADDTPLAVMGDSAGGNLAATLAL
                     RARDRGGPDIALQILLYPVLSPDFERESYRRCGQGDYLISTDDMRWFWDHHVAEADRA
                     NPEAAPLAATDFSNLPDAIIVVGGYDPLHDEGVAYAEALWAAGGAVVLRDHPEMVHGF
                     FTLVDLLAAANAEVAAVGSLIAGRLRAA"
     gene            complement(4457..5482)
                     /locus_tag="VN034_05095"
     CDS             complement(4457..5482)
                     /locus_tag="VN034_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWV60005.1"
                     /translation="MLHLADDTATAASATGDTVPTTSASASLSPGVPRDVATLRTLRI
                     AKHFPFFLAVAEEQNLHRAAERLNIAQSALSRRIADLERELGNIELFERQARGVAITK
                     AGQVLATDVRRILLQIEDARRNTRRIAEGDTGVLRVAFSEAMLRRPVLPAVLREFRAL
                     YPEVELRAFPLTSDAQRSRLLTDEIDVAFVIDEATDADQFARLTVGTDHFVLVLPEDH
                     PLAAKSAVGLAELANEPLLWPARHTSPRLFDRMIAAFDAQGVSPNIAVEVTAVDIAYE
                     LVAARIGLGVVTAARADRTPPGVVLRDLPDLDLPLPISMLWPKGSASPLIARFVALVE
                     SRVAEAG"
     gene            complement(5466..5774)
                     /locus_tag="VN034_05100"
     CDS             complement(5466..5774)
                     /locus_tag="VN034_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60006.1"
                     /translation="MTMHVMPLRFADIRLGQPIEFDGDVSGEGPMDQRLCDMVRRWSG
                     AAAASLVSLQGPRENFAPEQIAGRVMARHLDGSSTADVEVVMRAQDVCAKAVVRVALG
                     "
     gene            5875..6465
                     /locus_tag="VN034_05105"
     CDS             5875..6465
                     /locus_tag="VN034_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HWV60007.1"
                     /translation="MAATAQPIPAASAAADVLTRRRSPTPDALVAPGPTREETGRLIE
                     IALRVPDHGRLSPWRLVLIAGEAKQRWVDKLLELADRREDAPKTRVSTRKLASAPLVV
                     VVVSSAIAGHKVPEWEQWLSAGAVCMNLVNGAHALGFGANWLTGWHAYDEQATALLGL
                     EDGQRVAGVVLIGSVAAEAAERPRACAADVARWLEA"
     gene            complement(6510..7289)
                     /locus_tag="VN034_05110"
     CDS             complement(6510..7289)
                     /locus_tag="VN034_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HWV60008.1"
                     /translation="MSESTKKRRLRQPRVAEIVASSLRNRILSGALADGDMLPKQEEL
                     LNEFGVSPPCIREAFRILETEGLVTVIRGNVGGAIVHVPHPGTAAYMLGLVLQARSVS
                     LLDLLNGMRLLEPACAAEAALRADRGTTVLPKLRANIDACADAIDDPARFIVLARAFH
                     TELVSNCGNETMSLVVGALERLWTAQVEELAVDRSVHGSFSDRAVRASLLHEHERIYR
                     AIEDGDAPTAERAIREHFTGGGERRHGFDTGLTIKADMLRS"
     gene            7396..8313
                     /locus_tag="VN034_05115"
     CDS             7396..8313
                     /locus_tag="VN034_05115"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWV60009.1"
                     /translation="MSADASRYQAFTISITPFAEDGSIDEAKSRRHLSKLGDAGIGVY
                     VGGGGSGEGYTLSADETKRLLTIAVEELKGRAPIRAMGVEPRTARQMIDFLDMAAEAG
                     VDAAQVYSLDVGHGHVPTPRELDAYFSEVLSNSDVPSVLSTHQSVGYVIPAQVIIELF
                     DRHKQLIGLNISHQDPNYLRSLVDALGDRIDICVGGPHQAPLALAFGAQGFLSSEGNI
                     APKLCMSVIAAARAGDNALFLERWGKLMRLFMALYGNGGIRVTKALLEHFGLAGGVPR
                     APRLPAEADSLTKALAVVEEIGLAEIEGW"
     gene            complement(8368..9237)
                     /locus_tag="VN034_05120"
     CDS             complement(8368..9237)
                     /locus_tag="VN034_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HWV60010.1"
                     /translation="MNEEDLPYLQRGRQKVETTSSVLISSSKYMNDFRIREWVTTQCL
                     KRNGRDYPVTYGIPSLIDAVREVQDHERIEELFNEARRGWPEFDKWLDERWLARLTRE
                     DMKSCPEGSVGGIWYKWLVGAGMEVDIIPTFEPRSGFEYFFLRGVQIHDIMHILSGGG
                     FDGIGEIMPAFLKYGNLFRHFTPELAGLLNVQNTLLTIPMFMRGPLHYPELTLRMFEL
                     SHYAMKVGEQSDALFLPRYEDWLDLPLEEARRKFGVRGAQTIDTSRESAYWSEQVDRY
                     DPLPDGPEAVAAE"
     gene            complement(9271..11007)
                     /locus_tag="VN034_05125"
     CDS             complement(9271..11007)
                     /locus_tag="VN034_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV60011.1"
                     /translation="MTDILIQGGTVVDGTGAPSYAADVRVKGGLIAEIAPGLSPAAGE
                     RVVDAAGCIVSPGFIEPHTHMDAVMWWQPSLDPLPGYGVSTVVIGNCGFTAAPVHKDP
                     EVRMEMVKIFSFFEEVAEKPFVEMLPWDWNSWPEYRASVEAKCKTSTNIAGYAGHIAM
                     RLAVMGMDAWDRAATADEIQKMCDMLQEAIDAGALGMSSNLMDHDSKDRPVPSLVADD
                     AEWSGLIGVLARNPGSQLQVILDTFFKLKAPEQMERLGNLCRDRGVRVQMAGAGGLLH
                     FQDSIRDKMWEISARQKAEGLDFWPSFAHVPPTTALNFFSSSSFAQANEYVWHEVVQA
                     PTEEEKLALLHDADFRARARDSWDNKAFKQSLVANPHMMLLLDSETGVGPLGLNLKEL
                     AEQTGQHASDALADWVIANGVQSIVNRVPHPMHFDKTVELMKTSQTVANISDAGAHGQ
                     MLCGGGENILLLTHYLRNKELTLEEAIHVQTGKQAEHFNFDDRGVLKPGYRADIAVFN
                     LDEIEARPLRRAFDVPDGKGGFTWRFSRDAAPMRLTLVNGVPTFENGAFTGELPGQCI
                     GPVIRKVETAAA"
     gene            complement(11065..12207)
                     /gene="ribB"
                     /locus_tag="VN034_05130"
     CDS             complement(11065..12207)
                     /gene="ribB"
                     /locus_tag="VN034_05130"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWV60012.1"
                     /translation="MPALAPVQLPHSFLATPEEIIDEARNGRMYILVDDEDRENEGDL
                     IIPAQMATPDAINFMARFGRGLICLAMTQQRVDQLGLEPMSRRNGTQFETAFTVSIEA
                     REGVTTGISAADRARTIAVAIDASKGAEDIVTPGHVFPLLAREGGVLVRAGHTEAAVD
                     VARLAGLNAAGVICEILKDDGEMARLGDLIPFAQQHQLKIGTIRDLIAYRRRHDHLVE
                     RRAEMAFESLWGADWRIISYRNRITGGEVVALVKGQIDAAEPVMVRMHVHSPMADLFA
                     EQDDRSGLLGRAMTKIGEEGRGVVVVLSSTAPDLVACIIRARKAGREESGARTVAVRE
                     YGIGAQVLTDLGIHRITLLSNSDTNYVGLDGYGIEIVDQQPLGCDI"
     gene            complement(12317..12898)
                     /locus_tag="VN034_05135"
     CDS             complement(12317..12898)
                     /locus_tag="VN034_05135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWV60013.1"
                     /translation="MIRPIKIVGVGGTARIDSSSERVVCRALEAARALGAEVELFDGP
                     FIASLPLYIPGRTERTEAETRFVEAIRDCDGIIVGSPGYHGSLSGPVKNALDLLEDLA
                     RDGRPYLDGRAFGSIVTAFGWQACGTTLVALRSIAHALRAWPTPFGAAVNATVPLFGD
                     DGACLDPKVAEQLDIVGQQVMEFALWRRAGLAA"
     gene            13111..>14239
                     /locus_tag="VN034_05140"
     CDS             13111..>14239
                     /locus_tag="VN034_05140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWV60014.1"
                     /translation="MRFSLIFEAQTADASPRGERQVFDELVEQALLAEQLGFDVIWSV
                     EHTSLTNYAHMSAPETFLAYIAGRTSRIGIGHGVVCLMPAMNHPIKVAERIATLDILS
                     KGRVHFGIGKGGSQQEAGAYGYDLKTLQPLIDEAMYLIPRMFVEDEVEHNGPHITIPP
                     RPIHPKPFQEPHPLMYMACTNPETLARAGERGVGALVLGFGGPEEVAKKNQIYRDAWA
                     TRKTEDQVGYRPNQHLAALCPTIILDDAEEARKIGIRGQRYFYESLNYWYGGGPKPEP
                     EKWGEELLEEGTDTIITTKLASETLTMDLRPDEDGRRPSGGILSAKNAYGSVADCISY
                     VEQLAAAGADEILFMTNMGTVPQWAQLETLRKIGTHVIPHFR"
BASE COUNT         2571 a         4719 c         4449 g         2500 t
ORIGIN      
        1 gaccgccggc ggcagcgagg aggtcgactt tgtcgaagca atcgcgatgc gtctgccgct
       61 cgacgtaatc ctcgaattgc ttggcgttcc ggccgacgac cgcgaccaga tgtatctgtg
      121 gagcaacgcc gtcatcggca gcgaagaccc cgaatatggc aatgtcgacc tcccgcgcga
      181 agccatcctc gaggcacagc aggcgatgtt cggctatttc gcgcgcttca tcgccgcgcg
      241 ccgtgcggcg ccgcgcgagg atctggtcag cctgcttgtc gaggcacgca tcgacggcga
      301 gccgctcggc gaccgcgaaa tcctgggctt ctgctttctg ctcgccatcg ccgggaacga
      361 gacgacgcgc aatgcaacca gcggcgcgat gctcgccctg atcgagcacc ccgaagcccg
      421 cgccgcgctc accgccgatc cgtcgatcat ggtgcccgcg gtcgaggaat tgctgcgctg
      481 ggtcaccccg atcgtctata tggcgcgcac ggcgacgtgc gacgtcgaca tcgcgggaca
      541 gacggtgcgc gagggcgaga aggtcgtgct cttctaccct tccgcgaacc gcgacgagag
      601 catgttcgaa aacccgttca cgctcgacct gacgcgcaag cccaatcgcc atctgacctt
      661 cggcttcggg cgtcatagct gccttggcaa cgacctggca cggatcgaac tgcgcgcggt
      721 gctgaccgag ttgctcacac gcttcccggg gttggaactc gccggtcccg tcagccggct
      781 gcggtcgaat ttcgtcggcg gtatcaagca tatgccggtg cgactggcgc cttgatactg
      841 gacagcgctc tctcttctta ttagttaaag attataatga taggagaggg cgcgatggcc
      901 gactgggaca gtctttacgc aagccgcatg cacggggtga tcgcatcgga tattcgcgaa
      961 cggatgaagc tgctcgatgc cggcgagatc atccatctcg gcggcggcct gcccgatccc
     1021 gccatctttc cctatgccgc gctatcggcg gcatccgccg cgatctggtc cgatccggcg
     1081 cgggcgcgca ccgcgctgca atatgccccg agcgagggtc atgcgccgct gcgcacatgg
     1141 atcgccgagc ggatgacgaa ggaaggcgcc ccctgcaccg cggacaatct catgatcacc
     1201 aacgggtcgc agcagggact cgacttcatc gccaagctgc tgctctcgcc cggcgacagg
     1261 gcgatggtcg aagtgccctc gttcatcggc gcgctgcgcg ccttcgatgc ttatcagccg
     1321 cattatgtcg gcttgccgca agacagcgcg cggtgggacg ctgaagcgct cggcggcgcg
     1381 aaattctgct acgcggggcc cgatttccgt aatccgaccg gcacgaccat gacgcttggc
     1441 gaacgcatgg ccttgctcga cctggccgag gctaccggaa tggtgctggt cgaggacggc
     1501 tgttacgagg ctctgcgcta cgacggcgag ccagtcccct cgcttctcaa gctcgcgata
     1561 cgccgccacg gcgacatcga aaagtctccc gtcctccaca cgggcacctt ctcgaaaacg
     1621 atcgcgccgt cgctgcgcgt cggctggatc gccggacccg cgcacgtcat ccgcaagctc
     1681 gtgctgatca agcaggcgag cgaccttgca acaagcgcgc tcaatcagat gctgttgctc
     1741 gatgtggttg agaccgcgct cgacgacacc attgcgcgag cccgccgcgt ctatcgcgaa
     1801 cgccgcgact ccatgctcgc gtcgctcgcc gaggcgatgc cgcccggcgt caccttcacc
     1861 cggcccgagg gcggacttta tgtctgggcc gaactgccgg aggggatgga cgcgacgctg
     1921 ttcgcgcggc gcgcgctggc agaacacaag gtgtcgatca tcgccggcgg cgccttctac
     1981 ccggccggca ccgaaaagat accgggccat cgcaccagct tccgcctcag cttctcgatg
     2041 atcgacgccg cccgcgcacg cgaaggggtg atgcgtcttg gcgagcttgc ccgccagatg
     2101 atgcccgaaa cggtctgagg tcagtcggcg gccgcggtta tcacgatctc gagcgcatag
     2161 tcgggcgatg ccagcaccgt ctggatgctc gtccgcgttg gggcgcagcc cggaacgatc
     2221 cactggtccc acgcggcgtt gagcacgggc atgacggcca tgtcggagag gaggatcgcc
     2281 gccgtgagca ggcgcgaccg gtcgctcccc gcttcctcaa gcagcgcatc gatgcgcgca
     2341 agcacctcgt ccacctgatc agccaccgcc ccgccgcgat gatcgatcgc gatctgcccc
     2401 gaaagataga cgacgccggc atggacgacc gcgccgctca tccgcggatt ggcggcgatg
     2461 cggcgaatgt cacccatcga ccgtcaccaa cggcttcgcg cgctgcgaca attcatattg
     2521 cgactgctgc atgccggttt caggcactgt ttcatccgta cggtcgagga tatggtcgag
     2581 ccccgcgagc acatgttcgg ccgcatcgtc acccggctcg acggcgatcc cgcgcgtcag
     2641 cgtgatatgc gccgcctcga agctgcctgc gcccgcgcac atgatcatgc ccgtcggcgc
     2701 atcgtcgcaa acgagcgcaa gcattcccgg cgcgacgctc gccggcgaca gcgcagccag
     2761 atcgtcctcg gtcatgatgt cgacggtcat tcgcgtcgct gccgacggcg caagcgcgtt
     2821 gacgcgaatg ccatgcttgc gcccctcctg cgccagcgtc ttcatgaagc cgacaagccc
     2881 gagcttggcg gcgccgtact gcgcctgccc gaaattgccg tagagtcccg atgacgaggt
     2941 cgtcatcatg atccggccat ggccctgtgc ggtcataatc ccccacaccg ccttggtgca
     3001 atggaccgat cccgcgaggt ggacatcgat cacggtgaag aaatccgaaa gatcctgctt
     3061 ggcaaaagtc cggtcgcgca ggatgccggc gttgttgacg agaatatcga tgcggcccca
     3121 ttttcccatc acctcgccga ccatcgcttc gacctcggca aaatcgttga cgctcgcgcc
     3181 gttcgcgatc gcttcgccac ccgcctgacg gatctcctcg accacctgct cggccatcgt
     3241 cgcattgcca ccgctgccgt cgcgcgcgcc gccaaggtcg ttgacgacga ccttcgcgcc
     3301 gcgctttgcc agcaacagcg catgctcgcg gccaagaccg ccgcccgcgc ccgtgacgat
     3361 cgccacccgg ccgctcagat cgatccgcat ttcattctcc atccatttca ggtcaacctt
     3421 ggggagcgcc gaagcaaagg tcgaatatcg aacgcggatc gaagggatcg cgattgggca
     3481 attgcaccgg accgccccgc gtcccgagaa gaggggcaac aggaggaacc ttgatgagcc
     3541 tcgatcccga gatgcgcgcc atactcgatc ggcgcaaagc cctgaacctg ccgggctttt
     3601 cggcgggaac acccgtggac gcccgccggt ccttcgccga gtcgcaggcg gcgctgccgc
     3661 ccggacgcgg cgcccgcaat gtgacgatca gcaacgaagc gatcaccgga cccaagggcg
     3721 acgtgccgat ccggcgctat caccccgagg gtgaggcgca cgggcgcatc ctctatctcc
     3781 acggcggcgg ctgggttttc gggacgctcg acggcttcga tccggtgtgc cgtcaactgg
     3841 cgcgggagag cggcgctgaa attgtcagcg tcgactatcg cctggcgccc gaacatccct
     3901 tcccccagcc gctcgacgat tgctgggcgg cgcttctcgc gctcgctgac gacaccccgc
     3961 tggcggtgat gggcgacagc gccgggggca atctggccgc gacgctcgca ctgcgtgccc
     4021 gcgatcgcgg tggcccggac atcgcgctgc agatactgct ctaccctgta ttgtcacccg
     4081 atttcgaacg tgaatcctat cgtcgatgcg gccagggcga ttatctgatt tcgaccgatg
     4141 acatgcgttg gttctgggat catcatgtgg cggaagccga tcgcgccaat cccgaggcgg
     4201 ctccgctcgc ggcgacggac ttttcgaacc tccccgacgc gatcattgtc gtgggcggat
     4261 atgacccgct ccacgacgaa ggcgtcgcct atgccgaagc attgtgggcc gcgggcggag
     4321 cggttgtgct gcgcgaccat ccggagatgg tgcatggctt cttcacgctt gtcgaccttc
     4381 tcgcagccgc caatgccgaa gtcgccgcgg ttggatcgct gatcgccgga cggctccgcg
     4441 ctgcctgagc cgcggttcaa cccgcttcgg ccacgcggct ttcgaccagt gcgacgaagc
     4501 gcgcgatcaa cggcgaagca ctgcctttcg gccaaagcat gctgatcgga agcggcaggt
     4561 cgagatcggg caggtcgcgc agcacgaccc cgggcggtgt ccggtccgcg cgcgcggcgg
     4621 tgacgacgcc aagaccgatc cgcgccgcga ccagttcata ggcgatatcg accgccgtca
     4681 cctcgaccgc gatattgggc gaaacaccct gcgcgtcgaa ggcggctatc atgcggtcga
     4741 acaggcgcgg cgaggtatgg cgcgccggcc agagcaaagg ctcgtttgcc agttcggcga
     4801 ggccgacggc ggactttgcg gcaagcgggt gatcttcggg cagcaccagc acgaaatgat
     4861 cggtgcccac ggtcaggcgc gcgaactgat cggcgtcggt cgcttcgtcg atcacaaagg
     4921 caacgtcgat ttcgtcggtc aagaggcgcg aacgctgcgc atccgaggtc agcggaaagg
     4981 cgcgcagctc gacttcggga tagagcgcgc gaaattcgcg aagcaccgca ggcaataccg
     5041 ggcgccgcag catcgcttcg gaaaaggcga cgcgcagtac gcccgtgtca ccctcggcga
     5101 tgcggcgtgt gttgcggcgc gcatcctcga tctgcagcag gatacggcgg acgtcggtgg
     5161 caaggacctg tcccgccttg gtgatcgcga cgccgcgcgc ctgccgctcg aacaattcga
     5221 tattgccgag ttcgcgttcg agatcggcga tccgccgcga tagcgccgac tgggcgatgt
     5281 tgagccgctc ggcagcgcgg tggaggttct gctcctcggc gaccgcaaga aagaagggaa
     5341 aatgcttcgc gatccgcaag gtacggagcg tcgcaacgtc gcgggggaca cccggcgaaa
     5401 gcgaagcgga tgccgacgtt gtcggcacgg tatcgccggt ggcggatgcg gcggtcgccg
     5461 tatcgtcagc caagtgcaac acggaccacc gcctttgcgc agacatcctg cgcgcgcatc
     5521 acgacttcca catcggccgt gctgctgccg tcgagatgcc gcgccatcac acggcccgca
     5581 atttgttcgg gggcaaagtt ttcgcgaggg ccctgaagcg acaccagcga tgccgcggcg
     5641 gcgcccgacc agcggcgcac catatcgcac aggcgctgat ccatcgggcc ttcgcccgac
     5701 acgtcgccgt cgaattcgat cggctggcca aggcgtatgt cggcgaagcg aagcggcata
     5761 acgtgcatag tcaccctcat ccctaaatat gttatctttt aaatgttagc cgattcggat
     5821 acggcggtca aacggcgatc gccgacgctt cgtttcgaga ggaagatcat catcatggca
     5881 gccaccgccc aaccgatccc cgccgcttcc gccgccgccg acgttctgac gcgccgccga
     5941 tcaccgacgc cggatgcact ggtcgcgccg gggccgactc gcgaagagac cgggcggctg
     6001 atcgaaatcg cgctgcgcgt tcccgatcac ggccgactca gcccttggcg ccttgttctg
     6061 atcgcaggag aggcgaaaca acgctgggtc gacaagctgc ttgagctcgc cgaccggcgc
     6121 gaagatgccc cgaaaacgcg cgtcagtacg cgcaaactcg ccagcgcgcc actggtggtt
     6181 gttgtcgttt cgtcggcgat tgcggggcac aaggtccccg aatgggaaca atggctgtcg
     6241 gcaggcgcgg tgtgcatgaa cctcgtgaac ggcgcccacg ccttgggctt tggcgcaaac
     6301 tggcttaccg gctggcacgc ctatgacgaa caggcgaccg cgctgctggg actggaagat
     6361 ggccagaggg tcgcgggcgt cgtgctgatc ggcagcgtag ccgccgaagc ggccgaacgt
     6421 ccgcgcgcat gtgccgccga tgtcgcacgg tggctcgaag cctgagccca atgcggccga
     6481 ccacccttat gagtttgacg cggtcccgct caggaccgca gcatgtcggc cttgatcgtc
     6541 agcccggtat cgaagccgtg tcggcgctcg ccgccgccgg tgaaatgctc gcggatcgcg
     6601 cgctcggcgg tcggcgcgtc gccgtcctcg atcgcacgat agatgcgctc atgctcgtgg
     6661 agcagcgagg cacgcacggc gcggtccgaa aagctgccgt gcaccgagcg gtcgaccgcg
     6721 agttcctcga cctgcgcggt ccacagccgt tcgagcgcgc cgacgacgag gctcatcgtc
     6781 tcgttgccgc aattgctgac aagttcggta tgaaaggcgc gcgcaagcac gatgaagcgc
     6841 gcagggtcgt cgatcgcatc ggcacaggca tcgatattgg cgcgcaattt ggggagcacc
     6901 gtcgtcccgc gatcggcacg cagcgcggcc tcggcggcgc acgccggctc gagcagccgc
     6961 atgccgttga gcaggtcgag cagcgagacc gaacgcgcct ggagcaccag ccccagcata
     7021 taggccgcag tgcccggatg cgggacgtgg acgatcgcgc cgccgacgtt accgcgaatg
     7081 acggtcacca gcccctcggt ttcgaggatg cggaacgcct cgcggatgca cggcgggctg
     7141 acgccgaatt cgttgagcaa ctcttcctgt ttgggaagca tgtccccgtc tgccagcgcc
     7201 cccgacagga tacggttacg caggctcgac gccacaatct cggcaacgcg aggctggcgc
     7261 aaacgccgct tcttcgtgct ctcgctcaca aaccataccc ctgtttctgg ccgaagccga
     7321 agaacaagcc atacgcttcg cttcaatcat tgcaagtcca gcacgaaccg agaaattacc
     7381 tatgaggaga atatgatgag cgccgacgca tcccgttacc aggccttcac gatcagcatc
     7441 acgccttttg ccgaggacgg atcgatcgac gaggcgaagt cgcgccgcca tctttccaag
     7501 ctcggcgatg ccgggatcgg cgtctatgtc ggcggcggcg gcagcggcga gggctatacg
     7561 ctgtcggccg acgagacgaa gcgcctgctg accatcgcgg tcgaagaact caagggccgc
     7621 gcaccgatac gcgcgatggg ggttgaaccg cggacggcac ggcaaatgat cgacttcctc
     7681 gatatggccg ccgaagccgg ggtcgatgcg gcgcaggtct attcgctcga cgtcggtcat
     7741 ggccatgtcc cgaccccgcg cgagctcgac gcctatttca gcgaagtgct gtcgaacagc
     7801 gatgtcccat cggtcctctc gacgcaccag tcggtcggct atgtgatccc ggcgcaggtg
     7861 atcatcgagc tgttcgatcg ccacaagcag ctgatcggac tcaacatcag tcatcaggac
     7921 ccgaattatc ttcgctcgct cgtcgatgcg ctcggcgacc ggatcgacat ctgcgtcggg
     7981 ggtccgcacc aggcgccgct cgcgctcgca ttcggcgcgc agggcttttt gtcatcggaa
     8041 ggcaatatcg cgccgaagct ctgcatgagc gtgatcgcgg cggcgcgcgc cggcgacaat
     8101 gcgctgtttc tcgaacgctg gggcaagctg atgcgcctgt tcatggcgct ctacggcaat
     8161 ggcggaatcc gggtgaccaa agcgttgctc gagcatttcg gcctcgcggg cggcgtgccg
     8221 cgcgcaccgc ggctgcccgc cgaggcggat tcgctgacaa aagcgctcgc ggtggtcgag
     8281 gaaatcggcc tcgcggagat cgagggctgg taaaaaaatg ggcggcggga tcacccgccg
     8341 ccaagcagcc cccttcggct gactccatta ttccgcggcg accgcctccg gtccatcggg
     8401 cagcggatcg tagcgatcga cctgttcgct ccagtaagcg gactcgcgcg acgtatcgat
     8461 cgtctgcgca ccgcgcaccc cgaacttgcg gcgcgcctct tcgagcggca ggtcgagcca
     8521 gtcctcgtag cgcggcagga acagcgcatc ggactgttcg ccgaccttca tcgcatagtg
     8581 cgacagctcg aacatgcgca gcgtcagctc gggataatga agcgggccgc gcatgaacat
     8641 cgggatggtg agcagcgtgt tctgcacgtt aagcaggccc gcgagttctg gcgtgaagtg
     8701 acggaaaaga tttccatatt tcagaaaggc cggcatgatc tcgccgatcc cgtcaaagcc
     8761 gccaccgctc aatatgtgca tgatgtcgtg aatctggacg ccgcgcagga agaaatattc
     8821 gaatcccgat cgtggctcga aggtcgggat gatatcgacc tccatccccg cgccgaccag
     8881 ccatttatac cagatgccgc ctaccgatcc ttcggggcac gacttcatat cctcgcgcgt
     8941 cagccgggcg agccagcgtt cgtcgagcca tttgtcgaac tcgggccagc cgcgccgtgc
     9001 ctcgttgaac aactcctcga tccgctcatg atcctgcacc tcgcgcaccg cgtcgatcag
     9061 cgacgggatg ccataggtca ccggatagtc gcgaccgttt cgcttcaggc actgcgtcgt
     9121 gacccattca cggatccgga aatcattcat atatttggac gaactgatga gcacgctgct
     9181 cgtcgtttcg accttttgcc gtccgcgctg cagatacggc agatcttcct cgttcatcct
     9241 gtccctcctc ttatatttat tccagggcga tcaggcggcc gcggtctcga ccttgcggat
     9301 caccgggccg atgcactggc caggcagttc gccggtgaac gcaccgtttt cgaatgtcgg
     9361 cacgccattg accagcgtca gccgcatcgg cgccgcatca cgcgagaagc gccaggtaaa
     9421 gccgcccttt ccgtcgggta cgtcgaacgc gcgccgcagc ggccgcgcct cgatctcgtc
     9481 gaggttgaac accgcgatat cggcgcgata gccgggcttg agcacgccgc gatcgtcgaa
     9541 gttgaaatgc tcggcctgct tgcccgtctg gacgtggatc gcctcttcga gcgtcagttc
     9601 cttgttcctg agataatggg tgagcagcag gatattttcg ccgccgccgc acagcatctg
     9661 gccatgcgcg ccggcgtccg agatgttggc taccgtctgg ctggtcttca tcaactcgac
     9721 cgtcttgtcg aaatgcatcg ggtgcggcac gcggttgacg atcgactgga caccgttcgc
     9781 gatcacccag tcggccagcg cgtccgaggc atgctggccc gtctgttcgg ccagttcctt
     9841 gaggttgagc ccgagcgggc cgaccccggt ttcggaatcg agcagcagca tcatgtgcgg
     9901 attggcgacg agcgactgct tgaacgcctt gttgtcccag ctatcgcggg cgcgggcacg
     9961 gaaatccgca tcgtgcagca acgcgagctt ttcttcctcc gtcggcgcct gcacgacctc
    10021 gtgccagaca tattcattcg cctgagcaaa tgaagaactc gagaagaaat tgagcgccgt
    10081 cgtcggcggt acatgcgcaa agctcggcca gaaatcgaga ccttcggcct tttgccgtgc
    10141 gctgatttcc cacatcttgt cgcggatcga atcctggaag tggagcaacc cgcccgcgcc
    10201 cgccatttgc acgcgcacgc cgcggtcgcg gcacagattg ccgagccgct ccatctgctc
    10261 cggcgccttc agtttgaaga aggtgtcgag gatgacctgc aactggctgc ccggattgcg
    10321 cgcgagcacg ccgatcagcc cgctccattc ggcatcgtcg gcgacgaggc tcggcaccgg
    10381 ccggtccttg ctgtcatggt ccatcaggtt cgacgacatg ccgagcgcgc cggcgtcgat
    10441 cgcttcctgc agcatgtcgc acatcttctg gatttcatcg gccgtcgcgg cgcggtccca
    10501 tgcgtccatg cccattacgg caaggcgcat cgcgatatgg ccggcatagc ccgcgatatt
    10561 cgtcgacgtc ttacacttcg cctcgaccga cgcgcgatat tcgggccagc tgttccagtc
    10621 ccacggcagc atttcgacga agggcttttc ggccacctcc tcgaagaagg agaatatctt
    10681 caccatctcc atccgcactt cgggatcctt gtggacgggc gcggcggtga agccgcaatt
    10741 gccgatcacg acggtgctga cgccatagcc gggtagcgga tcgaggctcg gctgccacca
    10801 catcaccgcg tccatatgcg tgtgcggttc gatgaagccc ggcgagacga tgcaacccgc
    10861 ggcatcgacg acgcgctcgc ccgcggcggg cgacaggccc ggggcgattt cggcgatcag
    10921 gccgcccttc acacgaacgt cggcagcata ggaaggcgcg ccggtgccgt cgacgacggt
    10981 ccctccctga ataagaatgt cggtcatcgc tctctccttt ttctgattct gtgataacgc
    11041 catattcttt ctaacattgc aatgttagat gtcacaaccg aggggctgct gatcgacaat
    11101 ctcgatcccg tagccgtcga ggccgacgta attggtgtcg ctgttgctga gtaaagtgat
    11161 gcggtggatg ccgaggtcgg tgagcacctg cgcaccgatg ccatattcgc gcaccgccac
    11221 tgtccgagcg ccgctttcct cgcgccccgc tttgcgcgcg cggatgatgc aggcgacgag
    11281 gtcgggcgcg gtcgagctca gcacaacaac aacgccgcga ccttcctcac cgatcttcgt
    11341 catcgccctg ccgagaagac ccgaccggtc gtcctgttcg gcgaacaggt cggccatcgg
    11401 cgaatggacg tgcatgcgca ccatcaccgg ttcggcggcg tcgatctgac ccttgaccag
    11461 cgcgactacc tcgccgccgg taatgcggtt gcgatagctg atgatgcgcc agtcggcgcc
    11521 ccagaggctc tcgaatgcca tttcggcgcg gcgctcgacc agatgatcgt ggcggcggcg
    11581 ataggcgatc agatcgcgga tcgtcccgat cttcaactgg tgctgctgcg caaaagggat
    11641 gaggtcgccc aagcgcgcca tctcgccatc gtccttcaat atctcgcaga tcacccccgc
    11701 cgcgttcagc ccggcgaggc gcgcgacgtc gaccgccgcc tcggtatgcc ccgcccgcac
    11761 cagcaccccg ccctcgcgcg cgagcaacgg aaagacatgt ccgggggtca cgatgtcctc
    11821 ggcgcccttc gaggcatcga tcgccaccgc aatcgtgcgc gcgcgatcgg cggcggagat
    11881 gcccgtggtt acaccttcgc gcgcctcgat cgacacggtg aaggcggttt cgaactgcgt
    11941 cccgttgcga cggctcatcg gttcgagccc gagctggtcg acgcgctgct gcgtcatcgc
    12001 cagacagatc aggccgcgcc cgaagcgcgc catgaagttg atcgcgtcgg gcgtcgccat
    12061 ctgcgcgggg atgatcaggt cgccctcatt ctcgcgatcc tcatcatcga cgaggatata
    12121 catgcgaccg ttgcgcgctt cgtcgatgat ttcctcgggc gtcgcgagga aggagtgcgg
    12181 cagctggacc ggagcgagcg cgggcatcat gatattcctg tgctgaagcg gcgtcgccgc
    12241 ctgtcgccca ttcgttatct ccgctgcccc cgcggctcta ttgccattgg gggatcgtca
    12301 ccgtcgctac ccggcatcat gccgcgagcc ccgccctccg ccacagcgcg aattccatga
    12361 cctgctggcc gacgatatcg agctgttcgg cgacctttgg atcaagacat gcaccatcgt
    12421 caccgaacag gggtaccgtt gcattgaccg cggcaccgaa cggcgtcggc caggcgcgca
    12481 acgcgtgcgc gatcgagcgc agcgcgacga gcgtcgttcc gcacgcctgc cagccaaagg
    12541 cggtgacgat cgatccgaag gcgcgaccat cgagataggg ccggccgtcc cttgccagat
    12601 cctcaagcag gtcgagcgca ttcttcaccg ggcccgacag gctgccatga tagccgggcg
    12661 atccgacgat aatgccgtcg cagtcgcgga tcgcttcgac aaagcgcgtc tctgcctcgg
    12721 tccgttcggt gcgcccgggt atatagagcg gcaaggatgc gatgaagggg ccgtcgaaca
    12781 gctcgacctc ggcgcccagt gcgcgcgcag cttcgagcgc gcggcacacg acgcgctcac
    12841 tgctcgaatc gatacgcgcg gttccgccta cgcccacaat ctttatcggc cggatcatct
    12901 gcccatctcc ttcaaaccct ttggcgttcg actaaggcgc agcagcccgt ggggtcgagt
    12961 gcccaaggcg gaccccagcg atctaaaatg ggcaagaaaa gggctcagcg atgctttgcc
    13021 gctaaccctc gtgccgccaa tcggcatttg aaagcatcgc gctatccgtc gatttttgaa
    13081 ggcgaagatt gggagcttga aagacacatt atgcgctttt cgttgatatt cgaagcccag
    13141 acggccgacg cctcacctcg cggcgaaagg caggtgttcg acgagttggt cgagcaggcg
    13201 ctactcgccg aacagctcgg gttcgacgtg atctggagcg tcgagcacac atcgctgacc
    13261 aactatgcgc atatgagcgc acccgagaca ttccttgcct atatcgccgg gcgcacgtcg
    13321 cggatcggga ttgggcacgg ggtcgtgtgc ctgatgcccg cgatgaatca cccgatcaag
    13381 gtcgccgaac gcatcgcgac gctcgatatc ctgtcgaaag gccgcgtcca tttcggcatc
    13441 ggcaagggcg gcagccagca ggaggcgggc gcctatggct atgatctcaa gacgctgcag
    13501 ccgctgatcg acgaggcgat gtacctgatc ccgcgcatgt tcgttgaaga tgaggtcgag
    13561 cacaacggcc cgcacatcac catcccgccg cggccgatcc atcccaagcc gttccaggaa
    13621 ccgcacccgc tgatgtatat ggcgtgcacc aatcccgaga cgctggcgcg cgccggcgaa
    13681 cgcggggtcg gggcgctcgt cctcggcttc ggcggccccg aagaggtcgc caagaagaac
    13741 cagatctatc gtgacgcctg ggcgacgcgc aaaaccgagg atcaggtcgg ctatcgcccc
    13801 aaccagcatc ttgcggcact gtgcccgacg atcatcctcg acgacgcaga ggaagcgcgc
    13861 aagatcggca tccgcggcca gcgctatttc tatgaatcgc tgaattactg gtacggcggc
    13921 ggcccgaagc ccgagcctga gaaatggggc gaggaattgc tggaggaagg caccgacacc
    13981 atcattacga cgaagctcgc gtcggaaacg ctgacgatgg acctgcgccc cgacgaggac
    14041 ggccgccggc cgagcggcgg catattgagc gcgaaaaacg cctatggcag cgtcgccgat
    14101 tgcatcagct atgtcgaaca gctggccgcg gcgggcgccg acgaaatcct gttcatgacc
    14161 aacatgggca ccgtgccgca gtgggcgcag ctcgaaacgc tacgcaagat cggtacgcac
    14221 gtcatcccgc acttccgat
//
LOCUS       DATMSU010000200         1534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_164432,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000200 DATMSU010000000
VERSION     DATMSU010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164432"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            594..>1534
                     /locus_tag="VN034_05145"
     CDS             594..>1534
                     /locus_tag="VN034_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWV60015.1"
                     /translation="MTSSSSQPVKAVLGPTNTGKTHLAVERLTAHSSGMIGFPLRLLA
                     REVYDRVVAIKGPAQVGLITGEERILPPEARYLLGTMEALPVTRDVAFVGIDEAQLGA
                     DPERGHVFTDRLLRARGREETMILGSASIKGLVRGLVPEAEIITRPRFSTLSYAGSSK
                     LSRLPKRSAVVAFSAEEVYAIAEMLRRFAGGAAVVMGALSPKTRNAQVAMFEAGEVDY
                     LVATDAIGMGLNLDVQHVAFASLQKFDGRRLRRLTISEMAQIAGRAGRHQQDGSFGTV
                     GLPQGGFTPEEIHAIEGHHFPPLASLFWRNPTPRFGSL"
BASE COUNT          259 a          514 c          504 g          257 t
ORIGIN      
        1 gccaccattg ggccgaaccg atatggtcac caaggtcggg aacgaggtcg aactgcgcga
       61 gccggtcgcg ccagccgaga gacggttcgg cgtcggagtc gctgcgtcgc agcgggatcg
      121 cttgcgacat cgtgagggcg gccgcgttgc ccgacatccc cgcaatgggt tcgtgacgct
      181 gaaacaaagt gcaacccccg ctcggcggac ctccctatac ccggccgccg aatgatgttt
      241 tcgaaggctt gtctgtaaag aaagaccgtt aaagtcaatt taatggcgga tttgccgcgc
      301 tgcgttgcgg cgaggcgtgg cggggtcggg atattttgca agatgcaccg acccaccctt
      361 ccccctggat gggggaagga tacgaagcct tgccgcgaag cggctaggcg aagttggatg
      421 ggggtgaggt cgcgaccttg caccacgcat cgatggcacc cctcaccgct gcgactagcg
      481 aacaagttgg caagtctcgc tgcctccccc atcgaggggg agggaaaaga tgtcgcctcg
      541 acgatcggct gttgcgcgtc gaccgcgccc atgccagcgt gtcctccaag aaaatgacct
      601 cttcctcttc ccagccggtc aaggctgtcc ttggccccac caacaccggc aaaacccatt
      661 tggcggtcga gcgcctgacc gcccattcga gcggcatgat cggctttccg ctgcgcctgc
      721 tcgcgcgcga ggtctatgac cgcgtcgtgg cgatcaaggg accggcgcag gtcgggctga
      781 tcaccggcga ggagcgaatc ctgccgcccg aggcgcgcta tctgctcggc acgatggagg
      841 cgctgccggt gacgcgcgac gtcgccttcg tcggcatcga cgaggcgcag ctcggcgccg
      901 atcccgagcg cggccatgtc ttcaccgacc ggctgctgcg cgcgcgcggg cgcgaggaaa
      961 cgatgatcct cggctcggcg agcatcaagg gcctcgtccg cggccttgtc cccgaggccg
     1021 aaatcatcac ccgcccgcgc ttttcgacgc tgagctatgc cggatcgtcg aagctgtcgc
     1081 gtctgcccaa acgctcggcg gtcgtcgcct tctcggccga agaggtctac gcgatcgccg
     1141 aaatgctgcg ccgcttcgcc ggcggtgccg cggtggtgat gggcgcactc agccccaaga
     1201 cgcgcaacgc gcaggtcgcg atgttcgagg cgggcgaggt cgattatctg gtcgccaccg
     1261 acgcgatcgg catggggctc aacctcgacg tccagcatgt cgccttcgcg agcctgcaga
     1321 aattcgacgg acggcgcctg cgccgcctga cgatttcgga aatggcgcag atcgccgggc
     1381 gcgccggacg ccaccagcag gacggcagct tcggcaccgt cggcctgccg cagggcggct
     1441 tcacgcccga ggaaatccac gcgatcgagg gccatcattt cccgccgctc gccagcctgt
     1501 tctggcgcaa tccgacgccg cgcttcggtt cgct
//
LOCUS       DATMSU010000201        12386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_22202, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000201 DATMSU010000000
VERSION     DATMSU010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 12386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12386
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22202"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VN034_05150"
     CDS             <1..536
                     /locus_tag="VN034_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV60016.1"
                     /translation="PDLREAERRLAADTARIGVATADLYPRIQLGANGNVLRNDDVKG
                     SDSFSFSLGPLLSWSFPNSAVARSRLKQAKAQGDASLAAFDGKVIGALKEVEQALARY
                     DAGQQRHAALAEARDRAQTAYGLAQARYRAGSVSLLDTVIAQQSLIEARATLAASDQQ
                     LGSARVDLFKALGGGWS"
     gene            complement(705..1754)
                     /locus_tag="VN034_05155"
     CDS             complement(705..1754)
                     /locus_tag="VN034_05155"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV60017.1"
                     /translation="MEAEVMMEGPPMVHPIPHSLFDSADMGSQAALAMWKDSMDAIYD
                     VRLCADQAVFRARAEAFHFGEVVLTAYRCLAQTFDRSRSRIGRDGVDHYTLQFCLSGR
                     HGSRDGARDGEAGPGDLIIADLAQPHATATSDFDSLNLTVPRRLLAPLLHAPHAQCLR
                     LISGSAPLVGLFRNHLIGLFAAAPAINVEEAEALVPPTLALAAAVLNMDAPQNALQVV
                     DPLLTAQIHRLISDRAADPAITAESVAEWFGISRRKLYYLMEPHGGFTACVRERRLHL
                     AHAMLRDPLQSGKSVAEIAERCGFAWRTNFARSFRARFGVTPREARALAGQHALRSQG
                     DLTERHMWEWIQMLR"
     gene            complement(1922..5866)
                     /locus_tag="VN034_05160"
     CDS             complement(1922..5866)
                     /locus_tag="VN034_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HWV60018.1"
                     /translation="MIGSISLAGIASPAQAQCVENPAEVFTCSGSLDSTQTLNRPTVS
                     ITTTPGFTVDTISNGGGSSFEVNGQDSVSYIDLEGSRLAGGGAYFTSGTGDLTIVSNG
                     EIDSDLGLQGLGLATLNGGDINVTWNGHINNSSGEGVRVTGTGEVNLLLSSVYAQDGG
                     IEINQNGPGGVSLIAAGDVTSVSARGVVVNTGLASTGDIALDLKSVTASGRGVNVINR
                     GSGATFIRATGPISGILGIRATNDVGSGEMFVDVADISGVDAAFFGNNYGLSDTFIRT
                     RDVFSESTGLQMSNDVTAGNLTIITQDVMTGSTGISAVNQGTGATSVTVQGSVTAETG
                     GGVYVFNDLQAQNLSVWTQDVQTGTGSGIGVSQEGSGSAYVRADGKIASQGGTGIRVN
                     TGVQAASVTVAAAQDVFGEDWGVLVDHSGSGGVAVVVAGDIVGELEDGVWVNAGPASG
                     YVTLDLQAVSGGDYGIRVDNAGLGGTVIRAAGPILSNEIGIRAENEAGSGELLIDVAD
                     VSGVDAAIVAINNGGSNSFIRTSDLVSEGTALSVDNGASAGGLTVFTGNVQANGAGIS
                     VDNGGTGETLVVAEGVVTAVQGDGVSVSNSQLGQSLRIDVDTVMAGDNGVSASHSGTG
                     IVTVNTSASVIGAGGDGVRVQAGAQSTGTTVNASAVTGGQNGIAVNNFGGGDTVVRST
                     GWVVGNQQDGIAVNNGQNAGDLVIEAMHAVGAANGISALNQGAGLISVTAHGLVQGSN
                     AGINVSSLTGGDISIGNFGSIRGAQDLPTARAVRASGGAITLENAGVLLGTVELWGDS
                     VRLRNADVWNNSNGESLFSGADDQVTNLAGGLIFAAGDAFTAETTRWTGLERFDNEGS
                     LWLIDGGAGDTLLTPAASVFQAGSTLGVDLGGVASDVFRTEGTLEIQTGSKLVLNQVG
                     ALTLHQRYVVAEAQQGLTGAFDFDDLFLTAFAGLRDGYTPTQAYVEFSQLRALGDAGL
                     TPNQKAAAGGADSLPDGNPVKDALLMLPSDDIARDAFDQISGEVHATARTAIIEDSRL
                     PRDAILDRLADADGSGAIWGRVFEGGGVSDGNFNAARSHRDMRGAMMGIDRPLGEDAT
                     VGAAFGALKSELDVRRRASHGEVKSLHAMIYGGAQLGAWRVSGGVGYAWTSTETRRSI
                     AFPGFQDAAEAEYDGSLQQGFLEASYRMPLSGGWAEPFASVAAIRVRSDAFTESGGDA
                     ALGGEKISEDVVISTLGLRFETGPAGPFSVRGMAGWQRNWGDVDPVGRHRFDGGETFE
                     ILGAARSKNAGVARLEARLRLSPRVGFGLGYNGVLGSSGVDHAVTGVFRVAF"
     gene            6345..7355
                     /locus_tag="VN034_05165"
     CDS             6345..7355
                     /locus_tag="VN034_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV60019.1"
                     /translation="MTDEFAQQLTLALKALSMSRGRLASEMGVDKSLVGRWASGSVRP
                     SAYNLERLTRFLAEKRPGLTLLDWDRTLPEFASLFGVAEVVEARARIGAGLSGTLQDM
                     VRNAVDSHLGAYEGFWRTTHASVFQPGCFCQQHGMIRIENGELQFELGADGIRYGGSM
                     SPVEGQIFAIASDSVRRVPSFLILNVVPMPKILLMDGLLVAASSSLRIPTAYSIIFER
                     VGDLSGDRAIDDAHAEGLMQRPEFVDDDDVTEAVRQHLLRDFGPKAASEGGEMMLSAP
                     LTPRLAQILALSRSAEERPPGKPPQASMRPSARESAAAPRDTPPPIQALDGLSNFGAA
                     AG"
     gene            complement(7437..7952)
                     /locus_tag="VN034_05170"
     CDS             complement(7437..7952)
                     /locus_tag="VN034_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60020.1"
                     /translation="MASRFPFSTVVTRIQRLWSAENGASDQILGSPDKFMPLAAWRAS
                     SGLVRSRWERGTALLVASLIAAPAIASTLMEGSYVRVGRSCDALEMEDRLISNGYSVS
                     PPGQTCRIVSRTSTGGSYPIFNQRCTDSEGDSRFDVRVISPDRIEVNRPGRPAVAYRR
                     CRAQAAPLRER"
     gene            8953..9594
                     /locus_tag="VN034_05175"
     CDS             8953..9594
                     /locus_tag="VN034_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HWV60021.1"
                     /translation="MISGAAAAASALARSTTGPLAGPVPVREIAAVPLSRPDTQIRDA
                     ALDPQLLRKALAALDRHAKSIRHRDRIAIVDFSAPSSEPRLHFLDVESRRASRLLVAH
                     GSGSDPHHTGYAERFSNASGSNASSEGAFVTGDYYVGKHGRSQRLAGLDPTNDHAMER
                     AIVIHGAWYANEDMIAAHVKLGRSQGCFAVGETCLKTMFDHLGTGRLLYAART"
     gene            9691..11118
                     /locus_tag="VN034_05180"
     CDS             9691..11118
                     /locus_tag="VN034_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWV60022.1"
                     /translation="MRYGVIACSLLALSFSLSGCNSSSNSSQSGVTAENAPWTDATAK
                     QLREAIDRRAAHGLDRMRFEVPANTEDQAALTQSALGYAAALARGASDPAKLSDIYTV
                     PQPDPNLKTGLAEALAKGKVDDWLSGLAPRDDTYRALSKAYLALRKAGHAPATAIPDA
                     GKPLNPGASDPRIPAIARQLVASDYLERPAAQSDTYGPDMVNAVRRMQADYGIKPDGV
                     IGKEALEILNLSDSDRARTLAVAMERMRWLDRNPPATRIDVNLASARLAYWRDGKLAD
                     TRKVVVGKPETETPQLGSPIYRLVANPTWTVPRSIQNKEIAGKGSAYLRRNNMMWKDG
                     WIVQKPGPKNSLGLVKFDMQNEHQIYLHDTPAKELFSEVQRQRSHGCVRVEDALGFAA
                     MLARDEGISAEWEKARATGKESFVPLPKAIPVRLTYQTVLFDEAGEPVIRADPYGWND
                     RVSAALGFPASEGHRLRPSGSDVGP"
     gene            complement(11355..11501)
                     /locus_tag="VN034_05185"
     CDS             complement(11355..11501)
                     /locus_tag="VN034_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60023.1"
                     /translation="MSRGRKPEGKYDRVAPPLPHPSWRHELPAEREKPRRLTAKEFWD
                     RLGL"
     gene            complement(11651..11917)
                     /locus_tag="VN034_05190"
     CDS             complement(11651..11917)
                     /locus_tag="VN034_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60024.1"
                     /translation="MAVGDDYARGQRDGIRLALAVLEAEEAKWAALLGESRSWRTNRT
                     REVRHKTLQVAQRRVQTLLNRLSPKGAIAEVDSELVAALEKAGL"
     gene            complement(11965..>12386)
                     /locus_tag="VN034_05195"
     CDS             complement(11965..>12386)
                     /locus_tag="VN034_05195"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HWV60025.1"
                     /translation="EAAGMLLDMPLGGTLLGLIGLAFVAAAAQQATKAWTTEFMRDLA
                     PGAPSWVVPVGRAGLAARAVVFAVIGISLVKAGWFGSAREVKGLGDALYSLTENKWLY
                     LLVAAGLFLFGVHSFVEAAWRRIRDEDVVARLKSAAR"
BASE COUNT         2305 a         4141 c         3837 g         2103 t
ORIGIN      
        1 ggcccgacct gcgcgaggcc gaacggcgcc tggcggccga caccgcgcgc atcggcgtgg
       61 cgacggccga tctttatcca cgcatccaat tgggcgcgaa cggcaacgtc ctgcgtaacg
      121 acgatgtaaa gggcagcgac agcttcagct tttcgctggg gccgctgctg tcgtggagtt
      181 ttcccaacag cgcagtggca cggtcgcggc tgaaacaggc caaagcgcaa ggcgacgcgt
      241 cgctcgccgc gttcgacggt aaagtgatcg gcgcgctgaa ggaagtggag caagctcttg
      301 cgcgatacga cgcggggcaa cagcgtcacg ccgcgcttgc cgaagcgcgc gaccgcgcac
      361 agacggctta cggcttggcg caggctcgct atcgtgccgg atcggtgtcg ctgctcgaca
      421 cggtcatcgc acagcagtcg cttatcgaag cgcgcgccac actcgcggcg tcagaccagc
      481 aattgggttc ggcgcgggtc gatttgttca aagcactggg cggcggctgg agttgagacc
      541 aatacgcata agtccaaaag cacatgattg gccgattccg gacaggccgc ttcaaagctc
      601 gaaatctaat aagcggacga tctgcctatc gagattatac aactggatgg caccgaaccc
      661 aggcccgcat aacttttact ggaacgcgcg cgttggtaca gaactcagcg caacatctga
      721 atccattccc acatgtgccg ctcggttaga tcgccttggg aacgcagcgc atgctgcccc
      781 gccagcgcgc gcgcctccct tggcgtaacc ccaaatcggg cccgaaaaga tcgcgcaaaa
      841 ttggtccgcc aagcgaatcc gcagcgttct gcgatttcgg cgacgctttt cccgctttgc
      901 agcggatccc gcagcatagc atgcgcgaga tggagccgac gctcgcgaac gcaggccgta
      961 aatccgccgt gaggttccat gaggtagtag agtttgcggc gcgatatccc gaaccactcg
     1021 gcgacgctct cggcggttat ggccggatcc gcggcccggt cggatatcag acggtggatc
     1081 tgcgcggtca gaagcgggtc gacgacctga agggcgtttt gcggcgcgtc catattcaga
     1141 accgccgctg ccagcgcgag cgtcgggggc acaagcgctt ccgcttcctc gacatttatg
     1201 gccggcgcgg cggcgaacag gccgatcagg tgattgcgaa acagcccgac caaaggcgcc
     1261 gagcccgaga taaggcggag acattgcgcg tgcggagcgt ggagcagcgg cgcaagcaaa
     1321 cggcgcggga ccgtcaagtt gaggctatcg aaatcgctcg tggcggttgc atgcggctga
     1381 gcgagatcgg cgatgatcag gtcgccgggc cccgcttctc cgtccctcgc cccgtcccga
     1441 ctcccatgcc gccccgagag gcagaactgc agtgtataat gatcgacgcc gtccctcccg
     1501 atccgcgaac gcgaccgatc gaacgtttgt gccagacagc ggtaggcggt caaaaccacc
     1561 tcgccgaagt ggaacgcctc ggcgcgggcc cgaaagacgg cctggtcggc gcacagccgg
     1621 acgtcataga tcgcgtccat gctgtccttc cacatcgcca gggccgcttg gcttcccata
     1681 tcggcgctgt caaacaagct gtgcgggatg ggatgaacca ttggaggacc ctccatcatg
     1741 acttcggctt ccattcgcga cctgccccga aaggccgcac cgcttcggcc ggacgctatc
     1801 gtcattttgc cgcccggcca atgacatcgt gacgcacgcc aatccctccg gacggcgcgg
     1861 agccgcccgg agggtccggt cgggctcagg aacgaggacc cgaccgccgg cgccaaggcc
     1921 gtcaaaaggc gaccctgaac accccggtga ctgcgtggtc gacaccgctg ctgccgagaa
     1981 cgccattata gcccaagcca aagccgaccc gcggcgacaa tcgcaagcgc gcttccagcc
     2041 gggcgactcc ggcgttcttc gaccgggccg cacccagaat ttcgaatgtc tcgccgccgt
     2101 cgaagcgatg gcggccgacc ggatccacat cgccccagtt ccgctgccag ccggccatgc
     2161 cgcgcaccga gaaaggcccg gcgggcccgg tttcgaaccg caggccgagt gtcgagatca
     2221 cgacgtcctc gctgattttc tcgccgccca aggcggcgtc tccgccgctc tccgtgaagg
     2281 cgtcgctccg tacgcggatc gccgccacgc tggcgaaggg ctcggcccaa ccgccggaca
     2341 gaggcatgcg atagctggct tccaagaagc cctgctgcag ggacccgtca tattcggcct
     2401 cggcggcgtc ctgaaatccc gggaaggcga tcgagcggcg ggtctccgtc gaggtccaag
     2461 cgtagccgac cccgcccgac acccgccagg cacccagctg cgcaccgcca tatatcatcg
     2521 cgtgaaggct cttgacctcg ccgtggctgg cgcgacgacg cacatcaagc tcggacttca
     2581 acgcaccgaa agcggcgccc actgtcgcgt cctcgccaag cggccggtct atgcccatca
     2641 tcgcgccgcg catgtcgcgg tggctacgtg cggcattgaa attcccgtcg ctgaccccgc
     2701 cgccctcgaa gacccgcccc cagatcgcac cggagccatc ggcgtccgcc aggcgatcga
     2761 ggatggcgtc gcgcggcagc cgactgtcct cgattatagc cgtccgggcg gtcgcgtgga
     2821 cctcccccga tatttgatcg aaggcatcgc gggcgatgtc atccgagggc agcatcagca
     2881 gggcatcctt cacgggattg ccatcgggca agctgtcggc gccgccggcg gccgccttct
     2941 ggttcggcgt cagccccgca tcgcccagcg cccgcagctg gctgaactcc acataggcct
     3001 gggtgggcgt gtagccgtcc cgcagacccg cgaaggcggt cagaaagagg tcgtcgaagt
     3061 cgaaagcacc cgttaatcct tgctgcgcct cggccaccac atagcgctgg tgaagcgtca
     3121 acgcgccgac ctggttaaga accagtttgg aaccggtctg aatctccagc gtcccctcgg
     3181 tgcggaaaac atcggaggcg acgccgccga gatcgacacc caaggtcgag cccgcctgga
     3241 acaccgaggc cgccggggtc agcagagtat cgccagcgcc gccgtcaatc agccagagcg
     3301 agccttcgtt gtcaaagcgt tcgaggccgg tccaacgtgt ggtttccgcg gtgaaggcgt
     3361 cgcccgcagc gaaaatcaaa ccaccagcga gattcgtcac ctggtcgtca gcgcccgaga
     3421 acaggctctc gccgttgcta ttgttccaga cgtcggcgtt gcgcagccgg acgctgtcgc
     3481 cccacagctc gaccgtgccg agcaggacgc cggcgttctc gagcgtgatc gccccgcccg
     3541 aagcccgcac cgcccgggcg gtcggcagat cctgagcgcc gcggatgctg ccgaagttgc
     3601 cgatggagat gtccccgccc gtcaatgagg agacatttat gccagcattg ctgccctgga
     3661 ccagcccgtg ggcggtgacg ctgatgaggc ccgcgccctg gttcagggcg gaaatgccat
     3721 tcgcggcgcc gacagcgtgc atggcttcga tgacgaggtc gccggcgttc tggccgttat
     3781 tgacggcgat gccgtcctgc tgattgccga ccacccagcc cgtcgaacgc acgaccgtgt
     3841 caccgccgcc gaaattgttg accgcaatgc cgttctgacc gccggttacg gcggaggcgt
     3901 tcaccgtcgt tcctgtgctc tgggcgccag cttggacgcg gacgccatct ccgcccgcac
     3961 cgatcaccga ggccgacgtg ttgaccgtga cgatgccggt tcccgaatgg cttgccgaca
     4021 cgccgttgtc accggccatc accgtatcga catcgatgcg caggctctgg ccgagctggc
     4081 tgttcgagac gctgacgccg tcgccctgca ccgcggtcac cacgccttcg gcgacgacca
     4141 gcgtttcgcc agtcccgccg ttgtcgacgc tgatgcccgc cccgtttgcc tgaacattgc
     4201 cggtgaagac tgtcagtccg cctgcggacg cgccattgtc cacgctcagg gccgtgcctt
     4261 cggaaaccag atcgctcgtc cggatgaagc tgttggagcc gccgttgttg atcgcgacga
     4321 tcgccgcatc gacgcccgac acatcggcga catcgatgag cagttcgcct gatccggctt
     4381 cgttctcggc acgtatgccg atctcgttcg aaagaatggg accggcagcg cggatgacgg
     4441 ttcctccaag gccggcgtta tcgacccgga ttccgtagtc gccgccggaa accgcctgca
     4501 gatccaacgt gacgtatccg gacgccggac cggcgttcac ccaaacgcca tcctccagtt
     4561 cgccgacaat atcgccggcg acaacgactg cgactccgcc ggagccggaa tgatcgacaa
     4621 gcacacccca atcttcaccg aaaacatcct gcgccgccgc tacggtcacg ctggcagctt
     4681 gaacgccggt gttgacgcgg attcccgtac cgccctggct ggcgatcttt ccgtccgcgc
     4741 ggacataggc ggaaccgctt ccctcctgcg agacgccgat gccgctaccg gtgcccgtct
     4801 gaacgtcttg ggtccaaacc gagaggttct gcgcctggag atcgttgaag acatagacgc
     4861 cgccgccggt ttcggcggtc acgctgcctt gtaccgtcac ggaggtggcg cctgtgccct
     4921 ggttcacggc cgaaatgccg gtgctgccgg tcatgacgtc ctgggtgata atcgtgagat
     4981 tcccggccgt cacgtcattg ctcatctgca ggcccgtgct ttccgagaac acatcgcggg
     5041 tccggatgaa ggtgtcggac aggccgtaat tgttgccgaa gaaggcggca tcgacgccgc
     5101 tgatgtcggc aacgtcgacg aacatctcgc cagagcccac gtcgttcgtc gcccggatgc
     5161 cgaggatacc cgagatcggg cccgtcgccc ggatgaaggt ggccccggag ccgcgattga
     5221 tgacgttgac gccgcggcca gaggcagtga cgcttttcag atccagcgcg atatcgcctg
     5281 tggaagccag gccggtgttg accaccacgc cccgggcgct gacgctggtc acatcgcctg
     5341 ccgcgatcag gctcacgccg cccggaccgt tctggttgat ctcgatccca ccgtcctgcg
     5401 catagaccga cgagagcaac aggttgacct cgcccgtgcc cgtgacgcgg acgccctcgc
     5461 cactcgagtt gttgatatgg ccattccaag tgacgttgat gtcgccgcca ttcaaggtcg
     5521 cgaggcccag gccctgcaac ccgagatcgg aatcgatctc gccgtttgac acgatggtca
     5581 agtcgccggt gccggaagtg aaataagcac cgccgccggc caggcgcgac ccctcaaggt
     5641 cgatatagct gaccgaatcc tggccattca cctcaaagct ggatccgccg ccgttcgaga
     5701 tagtgtcgac ggtgaagccc ggggttgtcg tgatcgacac ggtcggccgg ttcaaagtct
     5761 gggtcgagtc cagcgatccc gagcaggtga agacctccgc gggattctcg acgcattggg
     5821 cctgggcggg cgaagcaatt cccgccaggc ttatcgaacc gatcatcaat gcgagaccgg
     5881 atgtggtacg ccgcaaaggc gataagcgtc ggcgatacgg cggcaaacta gggagcaaca
     5941 tagaggccat ggatctgatc tttcgttcct gatgggatat gtcgtccggc gttaacaggt
     6001 tcgaatctag gacgaactcg acgcctggcg gacgcgatcc atgatcaggg ttccttgtcc
     6061 tcacgccgag ttgcaacaaa cgtcgccgcg cgacacgtcg ccatgcaacg ggttgcaacc
     6121 gatggccgta acgaccggta ccggtgatac gatcgatgca catggtgata cattgtgccg
     6181 gatatccgac cggacgcttg cgcccggacc gccgcttcgg tgcaacgggg tcatggcaaa
     6241 tcggccgggc cgaatcttcc ggtcgaccga cgtcactatg gaggctgccg tttgcttgaa
     6301 gtccggtcca tcccatctca tgatcgtttg cgacgagacg gacgatgact gatgagttcg
     6361 cgcagcagct caccctggcg ctcaaggctc tctcgatgag ccggggccgg ctggcttcgg
     6421 aaatgggcgt cgacaagtcg ctggtcggac gctgggcttc gggcagcgta cggccctctg
     6481 cctataacct cgagcggctg acccgcttcc tggccgaaaa gaggcctggc ctgacgctgc
     6541 tggactggga tcgcacattg cccgaatttg catcgctgtt cggtgtggcg gaggtcgtcg
     6601 aagctcgagc gagaatcggc gccggcctgt ccggaacgtt gcaggacatg gtccgtaacg
     6661 ccgtggattc gcatctgggc gcctacgagg gcttctggcg caccacgcat gcctccgtgt
     6721 tccagccggg ctgcttctgc cagcagcacg ggatgatccg catagaaaat ggggagctgc
     6781 aattcgaact tggagcggat ggcattcgct acggtggatc gatgtcgccg gtggaaggcc
     6841 agatatttgc catcgcgagc gacagcgtgc gccgggtacc ctcttttctg atactcaacg
     6901 tcgtaccgat gcccaagatc ctcttgatgg acgggctgct ggtcgcggcc tccagttccc
     6961 tgcgcattcc cactgcttac tcgatcatat tcgagcgcgt cggcgatctg tcgggagatc
     7021 gcgcgatcga cgacgctcat gccgagggct tgatgcagcg ccccgagttc gttgacgacg
     7081 acgacgtaac ggaagcggta cgacagcatt tgctgcgcga cttcggcccc aaggcggcga
     7141 gtgaaggcgg agagatgatg ctcagcgcgc cgttgacgcc gcggcttgcg cagatcctcg
     7201 ccttgtcccg ctcggccgaa gaacggccgc cagggaaacc gccgcaagcg tcgatgcgtc
     7261 cgtcggcgcg ggagtcggcg gcggcgcccc gcgatactcc gccacccatt caggctctgg
     7321 acggtttgag caacttcggc gccgcggccg gatgaccggc acctttctgc tgaacattcc
     7381 ccgtgcggcc cgacgttgca atgccgcccg aacgacaatc gacgcctcgt cgacccttag
     7441 cgttcccgga gcggcgctgc ttgtgcgcgg cagcgacgat aggcaacggc tggccggccg
     7501 ggccgattga cttctatccg atcgggggaa atgacacgca cgtcgaaccg ggaatctcct
     7561 tcactgtcgg tgcaacgctg gttgaatatt ggataggagc cgcccgtcga ggttcgcgat
     7621 acgatgcggc aggtctgccc gggcggagaa acggaatacc cgttggaaat cagccggtct
     7681 tccatctcaa gcgcatcgca ggagcgccct acgcgcacat aggagccctc cataagggtt
     7741 gaggcgatgg ccggcgctgc gatcagactg gcgaccagca gagccgtccc cctctcccat
     7801 cggctgcgga ccaatccgct cgatgcgcgc cacgccgcca ggggcatgaa tttgtctgga
     7861 gaaccaagaa tctggtccga agctccgttc tccgcgctcc aaagacgttg gatgcgggtc
     7921 acgaccgttg aaaaggggaa acgcgatgcc atagatttat cctcgatccg cttgcagccc
     7981 catcggctga ggcgtgatcg ggtcgccacc tggggaagag cagccacttg cggatccatc
     8041 gcttgtcggg cagttgcaaa gtcggtcgcc agcggactca ccgacatgca acggttgcag
     8101 atctgggtcc tgggagcgct aaggcttgtc gcccaggata tccgcgcacg ccttattgcc
     8161 gatgcagcgt cagcttcggg gttcaagtca ccgatgagac gggaagaaaa ttgacatcgg
     8221 acatctttcg attccgacct cgcccgcgcc gtcacggaac atcggtttgc cctgccgaga
     8281 tcgagaaact gatcgacgcc attgccagcc atcacatcgc gaaaaggtcg tattcgatgg
     8341 gtatgccgtg agggtcgggt gctcgtccga atgtctggaa cggcattcag gcctgtgagc
     8401 tgcagatggc gaaccgcgct atttcgggag tgacaaccga gcctctctaa caagcgcaac
     8461 cgctaaactt ggccgctagc agactggcag cttctaaacc gaattaagga gaagcggacg
     8521 tttacgtttc gaggccactg ataagacttt gccctggcct tcgagaaacc gccgctgcga
     8581 attgcgaacg gtattgctca gggcgaggcg accaggcgcg aacgacggag ccactcggcc
     8641 gtcggctcgc gccaccatca gcgcctggaa gcgcaaaagc aaagttgcca ccagccagac
     8701 caaagcggca agcgtcgcgg cgcctgccgc cgcaagagtg cgcccatcag atgccgcgtt
     8761 ccgcgagcga ccagcggatc ggaccccaag cgcagggcat catctcgccg tccctgaacg
     8821 gttcggcgag agcccggcac ggtgcggcgc gtccggctct ggaagcgatc gtaacgccct
     8881 tcaaccccgg tgccgcgcgc ctatattggg cagatgatcg accagcccct tcgactgggc
     8941 cggcgaacct tgatgatatc cggtgcagcc gcggcggcca gcgctctcgc gcgctcgaca
     9001 accggaccgc ttgcaggccc cgtgccggtc cgcgagattg ccgcggtccc gctatcgcga
     9061 ccggacacgc agatcaggga cgccgcgctc gatccgcagt tgctgcgcaa ggcgctcgca
     9121 gcgctcgacc gtcatgccaa atcgatccgg catcgcgatc gcatcgcgat cgtcgacttt
     9181 tcggcgccat cgtcggaacc ccggcttcat ttcctcgacg ttgaaagccg ccgggcatcg
     9241 cgcctgctcg tcgcccatgg cagcgggtcc gatccgcacc acaccggcta tgccgaacgc
     9301 ttctcgaacg cgtctggctc gaatgcctcg agcgagggcg cattcgtgac cggcgactat
     9361 tatgtcggca agcatggccg atcgcagcgc ctcgccggtc tcgacccgac gaacgatcat
     9421 gcgatggagc gtgcgatcgt catccatggc gcgtggtatg cgaatgagga catgatcgcc
     9481 gcgcatgtca aactgggccg cagccagggc tgcttcgcgg tcggcgaaac ctgtctgaag
     9541 acgatgttcg atcatctcgg cacgggccgc ctactatacg cggcccgcac gtaggacatg
     9601 ctcagaagaa ataactgcgc attccctcct taaccggaac gctcccctgc actcccgggt
     9661 tagatgtcta agccccctga ggagaattat gtgcgttacg gtgtgattgc ctgttcgttg
     9721 ctcgccctgt ccttctcgct ttccggatgt aattcctcct ccaactcctc ccaatcgggc
     9781 gtaaccgccg aaaatgcccc ctggaccgac gcgacggcca aacagctgcg cgaggcgatc
     9841 gatcgccgcg ccgcgcacgg gctcgaccgg atgcgcttcg aggtgcccgc gaataccgag
     9901 gatcaggccg cgctcacgca aagcgcgctc ggctacgccg ccgcgctcgc ccgcggcgcg
     9961 tccgatcctg cgaagctcag cgacatctac accgtcccgc agccggaccc caatctcaag
    10021 actggcctcg ccgaggcgct tgccaagggc aaggtcgacg actggctctc gggcctcgcg
    10081 ccgcgggacg acacctatcg cgcgctctcg aaggcctatc tcgcgctccg taaagcgggc
    10141 catgccccgg ccacagcgat ccctgacgcc ggcaagccgc ttaatcccgg cgcgagcgat
    10201 ccgcgcatcc cggcgatcgc gcggcagctc gtcgcatccg attatctcga gcggccggcg
    10261 gcacaaagcg acacctatgg ccccgacatg gtgaacgccg tgcgccgcat gcaggctgat
    10321 tatggcatca agcccgacgg cgtgatcggg aaagaggcac ttgagatcct caacctctcc
    10381 gacagcgacc gggcgcgcac gctcgcggtc gcgatggagc gcatgcgctg gctcgaccga
    10441 aatcctcccg ccacccgcat cgacgtcaat ctcgcctcgg cgcggctcgc ctattggcgc
    10501 gacggcaagc tcgccgacac gcgcaaagtc gtcgtcggca agcccgaaac cgagacgccg
    10561 cagctcggat cgccgatcta ccggctggtc gccaacccga cctggaccgt cccgcgctcg
    10621 atccaaaaca aggagatcgc aggaaaaggc tccgcctatc ttcgccgcaa caatatgatg
    10681 tggaaggacg gctggatcgt ccagaagccg gggccgaaga attcgctcgg gctcgtcaag
    10741 ttcgacatgc agaacgagca tcagatctac cttcacgaca cgccagcgaa ggagctgttc
    10801 agcgaggtgc agcgccagcg cagtcatggc tgcgtccgcg tcgaggatgc gctgggcttc
    10861 gccgcgatgc tcgcgaggga tgagggcata tcggccgagt gggagaaagc gcgtgccaca
    10921 gggaaggaaa gcttcgttcc gctgcccaag gcgatcccgg tacgcctgac ttatcagacc
    10981 gtcctattcg acgaggcagg cgagcccgta atccgcgccg atccctatgg ctggaatgac
    11041 cgtgtgtcgg ccgcgctcgg cttccccgcc agcgaaggcc atcgcctccg tccatccggg
    11101 tcagatgtcg ggccctgacc cggtcaagac atcgcgagca aacagcgaac accggatccc
    11161 gcagccgtgc ctccacccaa aaaaatagcg cccgcccctg aacgggcggg cgccagatga
    11221 agatatctgt cctcgcggga ggaagagctc ttctgggtcg cccggcgtga ttagggcgac
    11281 tcgcggcgcc gtgattatac cgaagcgcca aaaaaggacg gacggtgtcg tctcgagccg
    11341 cctcgcaccc gcctttacaa tccgagacgg tcccagaact ccttcgcggt cagccgccgc
    11401 ggcttttctc gctcggcagg cagctcgtgc cgccacgacg ggtgcggcag cggcggcgca
    11461 acgcggtcat attttccttc gggcttccgg ccacggctca tcgtcgactc ctcgcgatgg
    11521 tgaaccttct gacaacgctc aagttccctt cccaaaagga tttttcggga gcgagacgag
    11581 ctagcggaac ggcgaacatg tggcgcgaat gcgcgtcttc gcccgcatgc gcacacacga
    11641 ccgatcgctg tcagagacct gctttttcga gagccgcgac aagctcgcta tcgacctcgg
    11701 caatggcgcc tttcggcgac aggcgattga gcagcgtctg cacgcgcctt tgcgcgacct
    11761 gcaatgtctt gtgccgcacc tcgcgggtcc ggttggtccg ccacgagcgg ctttcaccga
    11821 gcagcgccgc ccatttcgcc tcttcggcct ccagcacagc gagcgcgaga cggatgccgt
    11881 cgcgctgtcc gcgagcataa tcgtcaccca cagccattct ccagcagcgt cgcgtggccg
    11941 atcagcagcg aagcgcgcac ttgcctatcg cgcggcagac ttgaggcgcg ccaccacatc
    12001 ctcgtcgcgg atccggcgcc aggccgcctc gacgaagctg tgcacgccga acagaaacag
    12061 gccagcggcg acgaggagat agagccattt gttctcggtg agcgagtaca aggcatcgcc
    12121 gaggcccttg acctcgcgcg ccgatccgaa ccaccccgcc ttgacgagcg agatgccgat
    12181 gaccgcgaac accaccgcgc gagccgcgag accggcgcgt ccaacgggta cgacccatga
    12241 cggcgctccc ggcgcgaggt cgcgcatgaa ctcggtggtc caggccttgg tcgcctgctg
    12301 agccgccgcc gcgacaaagg cgagaccgat caagccgagc agggtgccgc ccagcggcat
    12361 atcgagcagc attcccgccg cttcct
//
LOCUS       DATMSU010000202         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_22215, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000202 DATMSU010000000
VERSION     DATMSU010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22215"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2052
                     /locus_tag="VN034_05200"
     CDS             <1..>2052
                     /locus_tag="VN034_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60026.1"
                     /translation="VVALGLDGGDFVSLSSSGSGGAGGLGSDSSFGEGNQSGNGGDGG
                     AGTGGTVRLIATGGTITSNGEAVDITASGLSGEGGSGGIGQGGGVDGALGISGQTTGG
                     RVVIETAVGASGPGQIDLGPTSIAANGDFAGRIELRSAGNISFAGLTAEAFGIAAPTN
                     NDTDSALAGIFVAPTGGTIASQGDVTLTTDGSIGVHAQSTGILDVDGTLTIDAGDQID
                     IRHDLREGDDPTLRASGSLTATAATSISGAPGSLIAADGALSLTTDGTIGVDGLAGGD
                     IILTSTGATTVEHAEAVNDFTATAASFATGLNSIITGGDIDISSPGAVDLGNSSAGGF
                     VQVSGQSIAFNNIDAGLSVSLSADGTAPGAEGISGGSIAAGGNINLFANSIALTGAVT
                     GDASFFASGGAVSVNSADVAGTISIFSADDLSGSFVAGGNIFLNSDGSIDASAQANGG
                     YVDGNGIVTEGNLFVVAAGDVALTDSSAARMFGVNAGGAASIDGGAAGEDMLIIAGTT
                     ASLTDVSVGDDLTIRAPGNIDAVNVSATGAGADTHILGFSSGSGSPTFTIGVGEGSEG
                     INGADIVMASANGAIDAAALSAGDDILLNAANGIAVNGATTLGLGTTGGDSSIRTQGG
                     ATTLAGINAFSDVIVDAAGLANLSGVVAAGRNATINAQDVTLAALTDPGGNAAATLSA
                     D"
BASE COUNT          339 a          719 c          708 g          286 t
ORIGIN      
        1 cggtcgtcgc gctcggcctc gatggcggcg acttcgtctc gctatcgagc agcggcagcg
       61 gcggcgcggg cggcctcggc agcgacagta gtttcggcga gggaaatcag tcgggtaacg
      121 gcggcgacgg cggcgcggga accggcggca ccgttcgcct gatcgcgacc ggcggaacca
      181 tcacctcgaa cggcgaagcg gtcgatatca ccgccagcgg cctttcgggc gaaggcggca
      241 gcggcggcat cggccagggt ggcggcgtgg atggcgccct tggcatttcg ggacagacca
      301 cgggcggccg cgtcgtgatc gaaacggccg tcggtgcctc gggtcccggc cagatcgacc
      361 tcggcccgac gagcatcgcg gcgaacggcg attttgccgg gcgcatcgaa ttgcgttcgg
      421 ccggcaatat cagcttcgcc ggcctcaccg ccgaagcctt cgggatcgct gccccgacca
      481 acaacgacac cgatagcgcg ctcgcgggaa tcttcgtcgc gccgaccggt ggcacgatcg
      541 ccagccaggg cgacgtcacg ctgaccaccg acggctcgat cggcgtccat gcccagtcga
      601 ccggcatcct cgacgtcgac ggcaccctga cgatcgacgc gggcgaccag atcgacatcc
      661 gccacgacct gcgcgaaggc gacgatccga cgctccgcgc gagcggaagc ctgaccgcga
      721 ccgcagccac cagcatcagc ggcgcgccgg gcagcctgat cgccgccgac ggcgcgctgt
      781 cgctgaccac cgacgggacg atcggcgtcg acggcctcgc gggcggcgac atcatactga
      841 ccagcaccgg cgcgacgacc gtcgagcatg ccgaagccgt caatgacttc accgcgaccg
      901 ccgcgagctt cgcgaccggg ctcaattcga tcatcaccgg cggcgatatc gatatcagct
      961 cgccgggcgc ggtcgatctc ggcaattcga gcgcgggcgg tttcgttcag gtttcgggac
     1021 aatcgatcgc gttcaacaat atcgacgcgg gcctgtcggt cagcctgtcg gcggacggga
     1081 cggcgccggg cgccgagggg atcagcggcg gatcgatcgc cgcgggcggc aacatcaacc
     1141 tgttcgcgaa cagcatcgcg ctgaccggcg cggtgacggg cgatgcgtcc ttcttcgctt
     1201 ccggcggcgc tgtgtcggtg aacagcgccg acgtcgccgg cacgatctcg atcttctcgg
     1261 ccgacgatct ttcgggcagc ttcgtcgccg gcgggaacat cttcctcaat tcggacggca
     1321 gcatcgatgc ctcggcgcag gcgaacggcg gctatgtcga tggcaacggg atcgtcaccg
     1381 aaggcaatct gttcgtcgtc gccgcgggcg acgtcgctct gaccgacagc tccgcagcgc
     1441 gcatgttcgg cgtgaacgcc gggggcgcgg catcgatcga tggcggcgcc gcgggcgagg
     1501 atatgctcat catcgccggg acgaccgcca gcctgaccga cgtcagcgtg ggcgacgacc
     1561 tcaccatccg cgcacctggg aacatcgacg cggtcaatgt cagcgcgacc ggcgcgggcg
     1621 cagacacgca tatcctcggc ttttcgtcgg gatcgggcag cccgaccttc acgatcggcg
     1681 tcggcgaggg aagcgaaggc atcaacggcg ccgacatcgt catggcctcg gcgaacggcg
     1741 cgatcgacgc cgccgcgctg tcggcgggcg acgacatatt gctgaatgcc gcgaacggca
     1801 tcgcggtcaa cggcgcgacc accctcgggc tcggcacgac cggcggcgac agcagcattc
     1861 gcacgcaggg cggcgccacg acgcttgcgg gtatcaatgc cttcagcgac gtgatcgtcg
     1921 atgcggccgg cctcgcgaac ctctccggcg tcgtcgccgc gggccgcaac gccacgatca
     1981 acgcacagga cgtcaccctc gccgcgctga ccgatcccgg cggcaacgcc gctgcgacgc
     2041 tgagcgccga cg
//
LOCUS       DATMSU010000203         4391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_155315,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000203 DATMSU010000000
VERSION     DATMSU010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155315"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VN034_05205"
     CDS             complement(<1..645)
                     /locus_tag="VN034_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60027.1"
                     /translation="MKLRVAAALMTAPLLTFAAPPPLAAQTEAAPSGYPALLALYEEF
                     RAFVPPPMVKGVPDYSPAAMARHYAGLGAFEARLKAIDDSGWPVAQRVDYMVVLAEMR
                     GLEFQHRVVQPWKHDPAFYSTTNLGFGPKMHGALAIPKLPLAADELAAFKARLEAVPE
                     ALRQARANLTDPRGDLARLGIAQKQVEVNVYGELAARAAKSCPTRPPPSSPNSAC"
     gene            complement(696..1865)
                     /locus_tag="VN034_05210"
     CDS             complement(696..1865)
                     /locus_tag="VN034_05210"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWV60028.1"
                     /translation="MKWPAQRLTAGSKKSFGMYDEAAKLDAAGADLIHLEFGRPHADT
                     PLHVKEAAKAALDAGIVHYGDFRGTLSFRQALAEKLKSFNRLDYGVDEILVTNGLTHA
                     SFAAFMAAIDPGDEVILLDPYYPQHIGKTELAGGKVVIAELDKANDFAISKEKIAAKI
                     SDKTRMIVLINPANPTGRVYSRAELQNVADLAIEHDLLVLSDEVYEYITYGGVEHVSI
                     ASLPGMRERTISCFAFTKAYSMDGWRIGYLTADAALMPAIMRITMTDVTHVNVFIQEG
                     ARAAVEGPQDAMLAMVEADRRKRDIVVQALNQMPGVTCPEPEGTIYAFPDISATGRSS
                     QDLAMAILKEAHVVVESGSFYGAGGEGHLRVCFGSESEARVAEAMERLTRFFNAL"
     gene            2008..4308
                     /locus_tag="VN034_05215"
     CDS             2008..4308
                     /locus_tag="VN034_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HWV60029.1"
                     /translation="MRIACVGGGPAGLYFAISMKLREPAHVIDIFERNRADDTFGWGV
                     VFSDQTVENLMANDPVSGATIRDEFAHWDDIDVHIHGECIRSSGHGFIGIGRKRLLAI
                     LQDRAHDLGIGLHFEHEASADLADWADYDLVIAADGANSRIRNAHAEHFDVDIQVKKN
                     KFFWFGTAKQFEAFTFAFERTEAGWVWAHAYRFDDELSTFIVEMEPETWGKLGLDRMD
                     QPEAIALCEKIFARYLDGNPLMSNATHLPGPQAWLNFRRIITGRWSHDKFILLGDAAH
                     TAHFSIGSGTKLALEDAIKLAEVLNRPGLGRAEALEEYQAERNIEVLKLQNSARNSTE
                     WFETLDRYLPFEPIQFAYSLLTRSQRVSHENLRLRDRSWLEGVERWFQSRAPGGRDEG
                     APPMFAPYKLREMAIENRIVVSPMAMYCATDGTPGDFHFVHYGARAQGGAGLVYTEMT
                     CVSPEARITPGCPGMYTPEHAAAWRRITEFVHRESKAKICLQLGHSGAKGSTRIGWEG
                     MDEPLGEGNWPLIAASDVAWSPGNQTPRAMTRDDMDVVRDQFVAATRMAIYAGFDMVE
                     MHAAHGYLLSSFITPLLNRRTDEYGGSLENRLRFPLEVFAAMRAAWPAERPMSVRISA
                     NDWMGDAGVTPDEAVLIAQAFDAAGADLIDVSAGQTWADCKPVYGRMFQTPFADQIRN
                     EARVSTMAVGNIFETDHVNSILAAGRADLVAIGRPHMIDPMWTLRAAAQHDYRGAAVP
                     PGYRNGISQLARNMKRAAEQEAALRG"
BASE COUNT          792 a         1433 c         1398 g          768 t
ORIGIN      
        1 gcatgccgag ttcggcgagg aggggggcgg ccgcgttggg caggatttcg cggcgcgcgc
       61 ggcgagttcg ccgtagacgt tcacctcgac ctgcttctgc gcgatcccca gccgcgcaag
      121 atcaccgcgc gggtcggtga ggttcgcccg cgcctgtcgc agcgcctccg gaaccgcttc
      181 gagccgggcc ttgaacgcgg ccagttcgtc cgccgccagc ggcagcttcg ggatcgcgag
      241 cgcgccgtgc atcttgggcc cgaagccgag gttggtcgtg ctgtagaagg cagggtcgtg
      301 cttccatggc tggacgacgc ggtgctggaa ttcgagcccg cgcatttcgg cgagcacgac
      361 catatagtcc acgcgctggg caaccggcca gccgctgtcg tcgatcgcct tcagccgcgc
      421 ctcgaatgcc ccgagcccgg cataatgccg cgccatcgcc gcgggcgaat aatcgggtac
      481 gcccttcacc atcggtggcg ggacgaaagc gcggaattcc tcgtaaagcg cgagcagcgc
      541 gggatagccc gacggcgcgg cctcggtttg tgcagcgagc ggcggcggag cggcgaaggt
      601 cagcaagggc gcggtcatca gcgcggcagc cacacgcaat ttcatcttca aagcctgtct
      661 cccgatcgca aaaattccgt tgccgcagcc ccttgtcaga gcgcgttgaa gaagcgcgtc
      721 agccgctcca tcgcctcggc cacccgcgct tcggattccg atccgaagca aacgcgcaga
      781 tgcccctcgc ccccggcgcc ataaaagctt cccgattcga cgacgacatg cgcttccttg
      841 aggatcgcca tcgccagatc ctgcgacgaa cgccccgtgg cgctgatgtc gggaaaggca
      901 tagatggtgc cctcgggctc ggggcaggtc acgcccggca tctggttcag cgcctgcacc
      961 acgatgtcgc gcttgcggcg gtcggcctcg accatcgcca gcatcgcgtc ctgcggcccc
     1021 tcgaccgcgg cgcgcgcgcc ttcctggatg aagacgttga cgtgcgtcac gtcggtcatc
     1081 gtgatccgca tgatcgccgg catcaacgcc gcgtcggcgg tcagataacc gatgcgccaa
     1141 ccgtccatcg aataggcctt ggtgaaggcg aagcagctga tcgtgcgctc gcgcatcccg
     1201 ggcaggctcg cgatgctgac atgctccacc ccgccatagg tgatatattc gtaaacctcg
     1261 tcggacagca cgagcagatc atgctcgatc gcaagatcgg cgacattctg cagttccgcg
     1321 cggctgtaga cgcggcccgt cggattcgcc gggttgatca gcacgatcat ccgcgtcttg
     1381 tcggagatct tcgccgcgat cttttccttc gaaatcgcga aatcattcgc cttgtcgagc
     1441 tcggcgatca cgaccttgcc gccggcaagt tcggtcttgc cgatatgctg gggataatag
     1501 ggatcgagca ggatgacctc gtcgcccgga tcgatcgcgg ccatgaaggc ggcgaaggag
     1561 gcgtgggtca gcccgttcgt cacgaggatt tcatcgacgc catagtcgag ccggttgaag
     1621 cttttcagct tttcggccag cgcctgccgg aacgacaatg ttccgcgaaa gtcgccatag
     1681 tggacgatgc cggcatcgag cgccgcctta gccgcctcct tgacgtgcaa gggcgtgtcg
     1741 gcgtgcggcc gcccgaattc gaggtggatt aggtcggcac ccgcggcgtc gagcttcgcc
     1801 gcctcgtcgt acatgccgaa gcttttcttc gatcccgccg tcaggcgctg tgccggccac
     1861 ttcatcaatc gctacctccc gatgattccc atacacgtga ataattgtgt gataaaggat
     1921 atttcaagct taaaatttta agcttgaagc atttcgctac gaagcctagg aatcgggcca
     1981 tcgatcaatg gcgatggagt cgttcgagtg aggatcgcat gtgtcggagg cgggccggca
     2041 ggcctgtatt tcgcgatttc gatgaagctg cgcgaacctg cgcacgtcat cgacatcttc
     2101 gagcgcaacc gcgccgacga caccttcggg tggggcgtcg tcttttcgga tcagacggtc
     2161 gagaatctga tggcgaacga ccccgtcagc ggcgcaacga tccgcgacga attcgcgcat
     2221 tgggacgata tcgacgttca tattcacggc gaatgcatcc gttcgtcggg acacggcttc
     2281 atcggcatcg ggcgcaagcg cctgctcgcg atcctgcagg accgcgccca cgacctgggt
     2341 atcgggctgc acttcgagca tgaagcgagc gcggacctcg ccgattgggc cgattatgat
     2401 ctggtgatcg ccgccgacgg cgcgaacagc cgcatccgca acgcccatgc cgagcatttc
     2461 gatgtcgaca tccaggtcaa gaagaacaaa ttcttctggt tcggcaccgc aaagcagttc
     2521 gaagccttca ccttcgcctt cgaacgcacc gaagcgggct gggtatgggc gcacgcctat
     2581 cgtttcgacg acgagctttc gaccttcatc gtcgagatgg agccggagac ctgggggaag
     2641 ctcggcctcg accggatgga ccagcccgaa gcgattgcgc tgtgcgaaaa gatcttcgcc
     2701 cgatatctgg acggcaatcc actgatgtcg aacgccaccc acctgcccgg gccgcaggcc
     2761 tggctcaact tccggcgcat catcaccggc cgctggtcgc acgacaagtt catcctgctc
     2821 ggcgacgccg cgcataccgc ccacttctcg atcggttcgg gcaccaagct cgcgctcgaa
     2881 gatgcgatca agctcgccga agtgctgaac cgccccggtc tgggccgcgc cgaagcgctc
     2941 gaggaatatc aggccgagcg caatattgag gtgctgaaac tgcagaacag cgcgcgcaac
     3001 tcgaccgaat ggttcgagac gctcgaccgc tatctgccgt tcgagccgat ccagttcgcc
     3061 tattcgttgc tcacccggtc gcagcgcgtc agccatgaaa atctgcgcct gcgcgacaga
     3121 agctggctcg aaggcgtcga gcgctggttc cagtcgcgcg cgcctggcgg acgcgacgag
     3181 ggcgcaccgc cgatgttcgc accgtacaag ctgcgcgaga tggcgatcga aaaccggatc
     3241 gtcgtctcgc cgatggcgat gtattgcgcg accgacggca cgccgggcga ttttcatttc
     3301 gtccattatg gcgcgcgcgc gcagggcggc gcggggctcg tctataccga aatgacctgc
     3361 gtctcgcccg aagcgcggat caccccgggc tgccccggca tgtacacgcc cgaacacgcc
     3421 gccgcctggc gccgcatcac cgaattcgtg caccgcgaaa gcaaggcgaa aatctgcctt
     3481 cagcttggcc attcgggcgc caagggatcg accaggatcg gttgggaagg aatggacgag
     3541 ccgctgggcg agggcaactg gccgctgatc gccgcatccg acgtggcgtg gagccccggc
     3601 aaccagacgc cgcgcgcgat gacccgcgac gatatggacg tggtgcgcga ccagttcgtc
     3661 gccgcgaccc gcatggcgat ctacgccggt ttcgatatgg tcgaaatgca cgccgcgcac
     3721 ggttatctgc tgtcgagctt catcacgccc ctgctcaacc ggcgcaccga cgaatatggc
     3781 ggcagcctcg aaaaccggct gcgcttcccg ctcgaagtct tcgccgcgat gcgcgccgct
     3841 tggcccgccg agcggccaat gtcggtgcgc atctcggcca acgactggat gggcgatgca
     3901 ggggtgaccc ccgacgaggc ggtgctgatc gcgcaggcgt tcgatgccgc gggggccgac
     3961 ctgatcgacg tgtcggcggg ccagacctgg gccgattgca agcccgtcta cggccgcatg
     4021 ttccagaccc cgttcgccga ccagatacgc aacgaggcgc gcgtctcgac gatggcggtc
     4081 ggcaacatct tcgaaaccga tcatgtcaat tcgatcctcg ccgccgggcg cgccgatctg
     4141 gtcgcgatcg ggcgtccgca tatgatcgac ccgatgtgga cgctgcgcgc ggcggcgcag
     4201 cacgactatc gcggcgccgc ggtgccgccg ggttaccgga acggaataag ccagctcgcc
     4261 cgcaacatga aacgcgcggc cgagcaggaa gcggcgttga gagggtagga tgcgactgaa
     4321 ggatatgcat gccgtcgtca ccggcggcgg atcggggatc ggcgccgcgg tcgcccatgc
     4381 gctcgccgcc g
//
LOCUS       DATMSU010000204         5035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145584,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000204 DATMSU010000000
VERSION     DATMSU010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5035
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145584"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="VN034_05220"
     CDS             <1..1347
                     /locus_tag="VN034_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWV60030.1"
                     /translation="GVRPATDYGFSCPQKPFPGDSQPIETETSEDCLTLNVWTGAESG
                     KPRPVMVWIHGGGLMNGGSATPSTDGRALARRGVVVVTFNYRLGRLGFFAHPALSAES
                     PGAPLANYGYMDQIAALQWVKANIAAFGGDPGNVTIFGESAGGGSVAMLMTSPLARGL
                     FDKAIIQSGGGRQVPIAMRGASADGGPSMEEIGESFARDAGIKDDVLAGLRALPADKV
                     IGGVNMVNNFDRKKSVNWSVDGVLLTADPTTVFLRGDQAKVPLIVGANSDELGSIPMI
                     GALAAYALSQFGEAAPLIKALYRRPDGDDDIKRLPGDAGFVEPARYMARRAAETGGKA
                     WHYSFGYVASAKREKLKGAPHASEIPYVFGNPDVIEGATEADRQMAAAVSAAWVRFAK
                     TGDPAGGGIVWPAYDPGSWPTLVWDAVPHTVANLDGARLDLIEQAYTQSIWSKLAQ"
     gene            1344..3320
                     /locus_tag="VN034_05225"
     CDS             1344..3320
                     /locus_tag="VN034_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HWV60031.1"
                     /translation="MTIDKAGPLHIDGTRLRDSDGREVILRGVNLGGDSKLPWPDGGT
                     HVPTDFSDHREVSFVGRPFPIEEAGEHLGRLARWGFNCLRLLTTWEAVEHAGPGIYDT
                     AYLDYFTEVVRRAGDHGFHVFIDFHQDVWSRMSGGSGAPGWTFDVVGLDFRRFQAADA
                     AIVMQAAVDFGNPDPHQASYPQMVWSSNYQLPANGIMWTLFWLGRHATPDFLIEGVNV
                     QDYLQRHYLGAVEQVAMRVRDMPHVIGFDTLNEPGTGWVGLPLSRSVTDPHHPSPVPL
                     KPGPVWTPLDCLAAAQGRRVTLPVLARGADGRLTAVDEREFNRNEISIWAEGRACPFE
                     AAGIYALTDGAVRPVAEHAFDHFEGRAISLADDCYAPFFHDVARTIRRHREDWILFAE
                     MDPFAAIKRRGYPEHMPGRWVNAAHWYDIAMLYSKRFSPQTSIDIVSGEPDRDLAGLG
                     RRYLRQLSGYAVQSEARGVPTLIGEFGIPYDLDDGRAYRDWAEGGRDTSIWDDHSSAL
                     SLMYDAIDALRIHATQWNYTASNRNDAWVGDGWNQEDLSIFSCDQIGDAADLDGGARA
                     LAGFSRPFVRAAQGIVAHMRFDGETRSFAATIDVDPMISAPTIIHLPRHVFGGDFRIE
                     IDEKVATWQFDARNRLLMVHGLVAGTLPIAVRVG"
     gene            complement(3412..4236)
                     /locus_tag="VN034_05230"
     CDS             complement(3412..4236)
                     /locus_tag="VN034_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60032.1"
                     /translation="MQRLAGRVALITGAGRGIGRAVALKLARDGARVVVNDMDEEPAD
                     DVVGAIRELGGEAIACVGNVTDTDFGDRFVGAALDRFGGLDIIVNNAGFTLDGVVQKM
                     ADGDFQTMLDLHVTAPFRIFRAASEPIRLFAKREAKEGREIFRKLVLVSSIAGTNGNA
                     GQIGYSSAKSALIGMTKTLSKEWGRYKVCANCVAFGLIETRLTKSHDDPASRVVIDGR
                     EIQSGIPAPILAKATSSIPLGRAGSADEAAGGVYLFCIPESDYVTGQVVTVSGGMN"
     gene            complement(4288..>5035)
                     /locus_tag="VN034_05235"
     CDS             complement(4288..>5035)
                     /locus_tag="VN034_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HWV60033.1"
                     /translation="VAAQLLGGGGREYQERYDVPDSIFAKVAVKARRHAANNPFAVFR
                     DPVTIEDVLASPRLYGPLTRLQACPPTCGAAAAVLTSESFARRHGLDTSVVIEAQAMT
                     TDGASTFEGASMMRIIGYDMMKQAADAVYTAAGVGPNDIDVAELHDCFTTNEVISYEA
                     LGLCGEGDGAGFVADEANTYGGRCVTNPSGGLLSKGHPLGATGLAQCYELTHQLRGSA
                     ERRQVPGARFGLQHNVGLGGACVVTLYGRN"
BASE COUNT          837 a         1591 c         1707 g          900 t
ORIGIN      
        1 ggcgtccggc cggcgaccga ttacggcttt tcgtgcccgc aaaagccctt tccgggcgac
       61 tcgcagccga tcgaaaccga gacgagcgaa gactgcctga cgctcaatgt ctggacgggc
      121 gccgagagcg gcaagccgcg ccccgtcatg gtgtggatcc acggcggcgg gttgatgaac
      181 ggcggatcgg cgacccccag caccgatggc cgcgcgctcg cgcggcgggg cgtggtcgtc
      241 gtcaccttca actatcgcct cggccggctc ggcttcttcg cacatccggc gctgtcggcc
      301 gaaagccccg gcgcgccgct cgccaactat ggctatatgg atcagatcgc ggcgctccaa
      361 tgggtgaaag cgaatatagc ggcgttcggc ggcgatcccg gcaatgtaac gatattcggt
      421 gaatcggcgg gcggcggatc ggtcgccatg ctgatgacat cgccgctcgc caggggattg
      481 ttcgacaagg cgatcatcca atcgggcggc gggcgtcagg tgccgatcgc catgcgcggc
      541 gcctccgccg acggcggtcc gtcgatggag gaaatcggcg agagctttgc gcgcgacgcg
      601 gggatcaagg acgacgtgct cgccggattg cgcgcgcttc ctgccgacaa ggtcatcggc
      661 ggcgtcaaca tggtcaataa tttcgatcgc aagaaatcgg tgaactggtc ggtcgacggc
      721 gttctgctca ccgccgaccc aacgaccgtc ttcctgcgcg gcgatcaggc caaagtgccg
      781 ctgatcgtgg gcgccaacag cgacgagctg ggatcgatcc cgatgatcgg cgcgctggcc
      841 gcctatgcac tgtcgcagtt cggcgaggcg gcgccgctga tcaaggcgct ctatcggcgc
      901 ccggacggcg acgacgacat caagcggctg ccgggcgacg ccgggttcgt cgagcccgcg
      961 cgctatatgg cacgccgcgc cgccgaaacc ggcggtaagg cgtggcatta cagcttcggc
     1021 tatgtcgcat cggcgaagcg cgagaagctg aagggggcgc cgcatgcgag cgaaatcccc
     1081 tatgtgttcg gcaaccccga cgtcatcgaa ggcgcgaccg aagccgatcg gcagatggcg
     1141 gcggccgtca gcgcggcgtg ggttcgcttc gccaagacgg gcgatcccgc tggcggcggg
     1201 atcgtctggc cggcctatga tccggggagc tggccgactc tggtgtggga cgcggtgccg
     1261 catacggtcg ccaacttaga cggcgcccgc ctcgacctga tcgagcaggc ctacacccaa
     1321 agcatctgga gcaagctggc ccaatgacga tcgacaaagc cggtccgctc catatcgacg
     1381 gcacgcgatt gcgcgacagc gacggccgcg aggtcatcct gcgcggcgtc aatctgggcg
     1441 gcgacagcaa gctgccctgg cccgatggcg gcacccacgt tccgaccgat ttttccgacc
     1501 accgcgaggt cagtttcgtc gggcggccgt tcccgatcga ggaggcgggc gaacatctcg
     1561 ggcggctggc gcgctggggt ttcaactgcc tgcggctgct gacgacatgg gaggcggtcg
     1621 aacatgcggg gcccggtatc tacgacacgg cctatctcga ttatttcact gaagtggtcc
     1681 gccgcgcggg cgaccatggc tttcatgtct tcatcgattt ccatcaggac gtctggagcc
     1741 gcatgtcggg cggttcgggc gcgccagggt ggactttcga cgtggtcggc ctcgatttcc
     1801 gccgttttca ggcggccgat gccgcgatcg tgatgcaggc ggcggtcgac ttcggcaatc
     1861 ccgatccgca tcaggcgagc tatccccaga tggtctggag ctcgaactac cagcttcccg
     1921 cgaacggaat catgtggacg ctgttctggc tggggcggca cgccaccccc gactttctga
     1981 tcgagggcgt gaatgtgcag gattatctgc agcgccatta tctgggcgcg gtcgagcagg
     2041 tggcgatgcg cgtgcgcgac atgccgcatg tcatcggctt cgacacgctg aacgagccgg
     2101 gtacgggctg ggttggcctg ccgttgtcgc gaagcgtcac cgatccgcat cacccgtcgc
     2161 cggtgccgct caagcccggc ccggtctgga cgccgctcga ctgccttgct gcggcgcagg
     2221 ggcggcgggt gacgctgccg gtgctggcgc gcggggcgga cggccgcctg acggcagtcg
     2281 acgaacgcga attcaacagg aacgaaatat cgatctgggc cgagggccgc gcgtgtccct
     2341 tcgaggcggc gggcatttac gcgctgacgg acggtgcggt gcggccggtc gcggagcacg
     2401 ccttcgatca tttcgaggga cgcgccattt cgcttgccga tgattgctat gcgcccttct
     2461 tccacgacgt cgcgcgcacg atacggcggc accgcgaaga ctggatcctt tttgccgaaa
     2521 tggacccttt cgcggcgatc aaaagacgcg gttatccgga gcatatgccg ggccgctggg
     2581 tcaatgccgc gcattggtac gacattgcga tgctctattc gaaacgattt tcgccgcaga
     2641 cctcgatcga tatcgtgagc ggcgagcccg accgcgatct tgcggggctc ggccggcgtt
     2701 atctgcgcca gctttcgggc tacgcggtgc agtcggaggc ccgcggagtc ccgacgctga
     2761 tcggcgagtt cggtattcca tacgaccttg acgatgggcg cgcctatcgg gactgggcgg
     2821 aaggaggtcg cgacacctcg atatgggacg accattcgtc cgcgctttcg ctgatgtacg
     2881 atgcgatcga tgcgctgcga atccacgcga cccagtggaa ttataccgcg tcgaaccgca
     2941 acgatgcatg ggtgggtgac ggttggaatc aggaagatct ttcgatcttc tcgtgcgacc
     3001 agatcggtga cgcggctgat ctcgatggcg gagcgcgtgc gctcgcgggg ttctcgcgcc
     3061 cctttgtccg cgcagcacaa gggattgtcg cacacatgcg cttcgatggt gaaacgcgga
     3121 gcttcgccgc gacgattgac gtcgatccca tgatttccgc tccgaccatt atccatcttc
     3181 cccgacacgt cttcggtgga gattttcgca tcgagatcga cgaaaaggtc gccacttggc
     3241 agtttgacgc gaggaatcgg cttctgatgg tgcacggtct ggtcgcgggc acgcttccga
     3301 tcgccgtacg cgttggctga tcctcttggc gaacgcgtgg acgagcagca cgggccgccc
     3361 aaccgcgggc accggacggt gggatgatcc gcaatcgggc ggcccgatcg gtcagttcat
     3421 gccgcccgac accgtcacga cctgcccggt gacataatcg ctctcgggaa tgcagaacag
     3481 atagacgccg cctgcggctt cgtctgcgga gcccgcgcgg ccgagcggga tcgacgaggt
     3541 cgccttcgcc aatatggggg cggggattcc cgactggatt tcgcggccgt cgatcacgac
     3601 gcgcgatgcc ggatcgtcgt gcgatttggt gaggcgggtt tcgatgaggc cgaaggcgac
     3661 gcagttcgcg cagaccttgt agcggcccca ttccttgctc agcgtcttgg tcattccgat
     3721 gagcgcggac ttggcgctcg aatagccgat ctgtccggcg ttgccgttcg tgcccgcaat
     3781 cgaggagaca agcacgagct tgcgaaagat ttcgcggcct tccttcgctt cgcgcttggc
     3841 gaacaggcga atgggctccg atgccgcgcg gaaaatccgg aacggtgcgg tgacgtgaag
     3901 gtcgagcatc gtctggaaat cgccatcggc catcttctgg acgacgccgt cgagcgtgaa
     3961 gcccgcattg ttgacgatga tgtcgaggcc gccgaagcga tcgagcgcgg cgccgacgaa
     4021 acgatcgcca aaatcggtgt cggtcacatt gccgacacag gcgatcgcct cgccgccaag
     4081 ttcgcggatc gcgccaacga cgtcatctgc tggctcttcg tccatgtcgt tcacaacgac
     4141 gcgcgcgccg tcgcgcgcca gcttcagcgc gaccgcgcgt ccgatgccgc ggccggcccc
     4201 ggtgatgagg gcgacgcgcc ctgcaagccg ttgcatcata acctccctgt tcattcaagt
     4261 cgctgcgcgc tgcgaaggcg cgcgagttca gttgcggccg tagagcgtga ccacgcaggc
     4321 gccgcctagg ccgacattat gctgcaggcc gaagcgagcg cccggtacct ggcggcgctc
     4381 ggcgctgccg cgcagttgat gggtgagttc gtaacattgc gcgaggccgg tcgcgccgag
     4441 agggtgtccc ttggacagga gaccgcctga cggattggtg acgcagcgtc cgccgtaggt
     4501 gttagcctcg tcggcaacga acccggcgcc gtcaccctcg ccgcagaggc cgagcgcttc
     4561 gtagctgatc acctcgttgg tcgtgaaaca gtcgtgcagt tcggcgacat cgatgtcgtt
     4621 ggggcccacc cctgccgcgg tataaaccgc gtccgcggct tgcttcatca tgtcgtagcc
     4681 gatgatcctc atcatcgacg cgccctcaaa ggtcgatgca ccgtcggtgg tcatcgcctg
     4741 cgcttcgatc acgaccgagg tgtcgagccc gtggcggcgc gcgaagcttt ccgatgtcag
     4801 caccgccgcc gctgcgccgc aagtgggcgg gcaggcctgc agccgggtga gggggccata
     4861 gagacgcggc gaggcgagga cgtcctcgat cgttacggga tcgcgaaaga ccgcgaaggg
     4921 attgttcgcg gcgtggcgcc gtgccttgac cgcgaccttg gcgaagatgc tatcggggac
     4981 gtcgtagcgc tcctgatatt cgcgcccgcc gccgccgagc agctgcgccg cgacc
//
LOCUS       DATMSU010000205         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_13058, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000205 DATMSU010000000
VERSION     DATMSU010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13058"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VN034_05240"
     CDS             complement(<1..357)
                     /locus_tag="VN034_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HWV60034.1"
                     /translation="MAEAQILTPVDDGSANRLPAPVLGGRLAPFTQFIRQPAIQRALP
                     AIAMTSAIGIAALAYFTMQAAPQAQLFAGLDDTDKAAVAEALQSQGIAHSIDSSTGAL
                     TVDADKLHQARIALAGQ"
     gene            complement(361..711)
                     /gene="fliE"
                     /locus_tag="VN034_05245"
     CDS             complement(361..711)
                     /gene="fliE"
                     /locus_tag="VN034_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HWV60035.1"
                     /translation="MSTIDPSRLLQMRSSILNQNQALQRAAGRGGIGAAEGGIEGGGA
                     PDFGAAINNALQQVNAQQSKASQITEAYERGDTHDIVSVMIERQKASLGFETTLQVRN
                     KLLSAYRDIMNMPV"
     gene            961..1839
                     /locus_tag="VN034_05250"
     CDS             961..1839
                     /locus_tag="VN034_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWV60036.1"
                     /translation="MTVINTNVSALRAQNNSRVASQMQSQAMERLSSGKRINSAKDDA
                     AGLAIATRMDASARGLTQAIRNANDGISLAQTADSAAGSVADILVRMRELAMQASTGT
                     LSDEDRALVQEEVTALVSQIGDVTTRTTFNGNKLLDGSVAAGFDIQTGLDAGQTVNIT
                     VGNLQAAAIGTMEDADSDPDTPMTVVSGSAVSDIDLSTAAGASDALAILDGAINTVAT
                     ERANLGAQQNRLSSAVDNLTSSVTNLSESKSRIEDADFSVESTNLAAAGILAQASTAM
                     LAQANQSSQGVMNLLR"
BASE COUNT          359 a          636 c          675 g          356 t
ORIGIN      
        1 ctgtccggcg agcgcgatgc gcgcctggtg gagcttgtcg gcgtcgacgg tgagcgcgcc
       61 ggtcgagctg tcgatgctgt gcgcgatgcc ctgcgactgc agcgcctcgg cgaccgctgc
      121 cttgtcggtg tcgtcgagcc cggcgaagag ctgcgcctgc ggcgccgcct gcatggtgaa
      181 ataggcgagc gcggcgatgc cgatcgccga ggtcatcgcg atcgcgggca gcgcgcgctg
      241 gatggcgggc tggcggatga attgcgtgaa gggtgcgagg cggccgccga ggacgggggc
      301 ggggaggcgg ttcgcggacc cgtcgtcgac gggggtcagg atctgtgctt cggccatgga
      361 ttacaccggc atgttcatga tgtcgcggta cgcggacagc agcttgttgc ggacttgcag
      421 cgtggtttcg aagccgagcg aggctttctg gcgctcgatc atcacgctga cgatgtcgtg
      481 cgtgtcgccc cgctcatagg cttcggtgat ctggctcgcc ttcgactgct gggcgttgac
      541 ctgctgcagc gcattgttga tcgcggcgcc gaaatcgggg gctccaccgc cttcgatgcc
      601 gccttcggcc gcgccgattc cgccgcgacc ggcggcgcgc tgcaaggcct ggttctgatt
      661 gaggatcgaa ctgcgcatct gcagcagccg gctcgggtca atcgtgctca ttgcttcttc
      721 atccgtcctg atgcgcggcg gtctgccgca cgcacaaaga agaatgcaaa gctcgtgcca
      781 aaaaaattaa tacaatgttt tcggtgattt agatcgtcat ggaaacggct gtcgacggct
      841 ttcggcattt ttttgacggg cggacttaag cgctggcgcg cgccgccgtt actgcattcg
      901 tgaccgctcc tcgagccctc acatcacggg aatggccatc gaggccggaa aggaacaaag
      961 atgactgtca tcaacaccaa tgtgagcgcg ctgcgcgccc agaataactc gcgtgtcgcg
     1021 agccaaatgc agtcgcaggc gatggaacgc ctgtcgagcg gcaagcgcat caacagcgcg
     1081 aaggacgacg ccgccggcct cgccatcgcg acccgcatgg acgccagcgc acgtggcctg
     1141 acccaggcga tccgcaacgc caacgacggt atctcgctcg cccagaccgc cgacagcgcc
     1201 gccggcagcg tcgcggacat cctcgtccgt atgcgcgaac tcgcgatgca ggcctcgacg
     1261 ggcacgctga gcgacgaaga ccgtgcgctg gtgcaggaag aagtcaccgc gctggtttcg
     1321 cagatcggcg acgtcacgac gcgcacgacc ttcaacggca acaagctgct cgacggtagc
     1381 gtcgccgccg gcttcgacat ccagacgggt ctcgacgccg gtcagaccgt gaacatcacg
     1441 gtcggcaacc tgcaggcggc cgcgatcggc acgatggaag acgcggacag cgatcccgac
     1501 accccgatga cggtcgtcag cggctcggcg gtttcggaca tcgatctttc gaccgcggcg
     1561 ggtgcgtcgg acgcgctggc gattctcgac ggggcgatca acacggtcgc gaccgagcgc
     1621 gccaacctcg gtgcccagca gaaccgcctg tcgtcggctg tcgacaattt gacgtcgagc
     1681 gtcacgaatc tgtcggaatc gaagtcgcgc atcgaagacg ccgatttctc ggtcgaatcg
     1741 acgaacctcg cggccgccgg catcctggcg caggcatcga ccgcgatgct cgcccaagcg
     1801 aaccagagct cgcagggcgt gatgaacctg ctccgctaag cttcgcgaaa atttcaggct
     1861 atattcggtt ggtcccttaa atagcctgat tggatgcccc ggcccccaca gccggggcat
     1921 cttcattttt ggcgagcggc gggtcgcgaa cgacgcggca gatttcggcc gattgttggc
     1981 atggccgcac aggcggcttt caaataggat tcgcgcagag gcgcag
//
LOCUS       DATMSU010000206         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_69850, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000206 DATMSU010000000
VERSION     DATMSU010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69850"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1607
                     /locus_tag="VN034_05255"
     CDS             336..1607
                     /locus_tag="VN034_05255"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HWV60037.1"
                     /translation="MTLNPSPVWHPFTQHGLGDPIPLIARAKDAKLYDANGNSWIDAI
                     SSWWVTTHGHAHPRIMAAIAKQTEKLDQLIFAGWTHEPAETLAAELIRITPAPLTRIF
                     FSDSGSTSVEVALKMALGYWYNIGEARSRILVLEHSYHGDTIGTMSVGERGVYNRAWQ
                     PLLFDVDTIPFPHEGMEQATLDALEAACAAKPAAFIVEPLILGAGGMLVYPAWVLAEM
                     RAICARHGVLFIADEVMTGWGRTGTRFACDQAGVIPDIVCLSKGLTGGAIPLAVTLCI
                     EPIFAAHFSEDRGKAFYHSSSYTANPIACAAANANLEIWRDEPVQARIDALADAQAAH
                     LSLLSHDPRAANPRRLGTIAALDIVVEDAGYLSNLAPRLIAYYRDRGILLRPLGNTLY
                     VMPPYCITADELAQVWDAIAASLDILGGSPG"
     gene            complement(1846..2748)
                     /locus_tag="VN034_05260"
     CDS             complement(1846..2748)
                     /locus_tag="VN034_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV60038.1"
                     /translation="MGAVSKSSGAMLMLAILAAPATGSLTAQAQEQAPEQAQQTSDSE
                     VMTTVYGQLPSPDQMTKGPKVEGIISARSDGRIQVTTADGNNTAISIAEYTKIKSSGG
                     FLGLDRDKLAANSLLNGLPVSVDTLQWDGGLVANEVSLKSKDLRTASMIHTGTDQRFA
                     EQTAATEALRGRVGDIDQYNVKATTNVNFDTGKAVLTEQAKADLCNAANQAEGMDNAL
                     LLVVGYTDSTGSQELNQELSEKRAGRVVNHLQQACGWKPYRMLSPTGMAEADPAADNT
                     TPEGKAQNRRVAVNILVSKGLDGM"
     gene            complement(2968..3453)
                     /locus_tag="VN034_05265"
     CDS             complement(2968..3453)
                     /locus_tag="VN034_05265"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HWV60039.1"
                     /translation="MKNQALFALLAAVLAPLSVAAFTPQAYAKPVYVGVDGGNIAVSG
                     YDAVSYFEEGGVPVKGDAAFAVEHDGAVYHFASAEHAAKFRADPAAFVPQYGGHCAWA
                     MSRGYLAPGDPLAYAIVDGKLYLNFNQEVKAKWDKDRAGFIAAAEKNWAAMPDDAKFG
                     E"
     gene            3620..>3846
                     /locus_tag="VN034_05270"
     CDS             3620..>3846
                     /locus_tag="VN034_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60040.1"
                     /translation="MTKKKAGLLPLLAALALAAPALAQEDDGSLAQSGTPPERTSVLY
                     TYGDEPCPEAQEGEIVVCAQRPESERYRVPK"
BASE COUNT          655 a         1344 c         1155 g          692 t
ORIGIN      
        1 gattccgctg ccaccccgct cctcctgatc cgtttcctac ctaaaacggc catcaaggca
       61 aaatgcgccc aacttttttt ggagcgggac gatcgaatga tacggcgggg acttcatcaa
      121 ggtggacata cacggcgccg cgttcgccgg cgccaagcat aatcgcgaat tgaagacaag
      181 gctccggagc gcattctgcg acagtgctta aatcgctaca tcaagcgcgt tttaacgtcg
      241 atattcccgc gaaggccggg atctcgacct tgcatttttc gcatcggcaa gaccccggcc
      301 ttcgccggga tgacgatatg ggatagggcg tacctatgac actcaaccca tcccccgtct
      361 ggcacccctt cacccagcac ggcctcggcg accccatccc gctgatcgcc cgcgccaaag
      421 acgccaaact ctacgacgcg aacggcaata gctggatcga cgccatctcg tcgtggtggg
      481 tgaccaccca cggccacgcc cacccgcgca tcatggccgc aatcgccaag cagaccgaaa
      541 agctcgacca gctcatcttc gccggctgga cgcacgagcc cgccgagacg ctcgccgccg
      601 aactgatccg catcaccccc gccccgctca cccgcatctt cttctcggac tcgggctcga
      661 ccagcgtcga ggtcgcactc aaaatggcgc tcggttactg gtataatatc ggcgaggcgc
      721 gcagccgcat cctcgtcctc gagcacagct atcatggcga cacgatcggc acgatgtcgg
      781 tcggcgagcg cggcgtctac aaccgcgcgt ggcagccgct cctcttcgac gtcgacacca
      841 tccccttccc gcacgaaggc atggagcagg cgacgctcga tgcgctcgaa gccgcctgcg
      901 cagccaaacc ggcggccttc atcgtcgaac cgctgatcct cggcgccggc ggcatgctcg
      961 tctaccccgc atgggtgctc gccgaaatgc gcgcgatctg cgcgcgccac ggcgtgctct
     1021 tcatcgccga cgaggtgatg accggctggg gccgcaccgg cacgcgcttc gcctgcgacc
     1081 aggcgggcgt catccccgac atcgtctgcc tgtcgaaggg gctcaccggc ggcgcgatcc
     1141 cgctcgcggt gacgctctgc atcgaaccga tcttcgcggc gcatttttcg gaagaccgcg
     1201 gcaaggcctt ctatcattcg tcgagctaca ccgcgaaccc gatcgcctgc gccgccgcca
     1261 acgccaatct cgaaatctgg cgcgacgagc cggtccaggc gcgtatcgac gcgctcgccg
     1321 acgcgcaggc cgcgcatctg tctttgcttt cacacgatcc gcgcgcggcg aacccgcgcc
     1381 gcctcggcac gatcgccgcg ctcgacatcg tcgtggaaga cgcgggctat ctttcgaatc
     1441 tcgcgccgcg cttgatcgct tattatcgcg accgcggcat actcctccgc ccgctcggca
     1501 acacgctcta cgtcatgccg ccctattgca ttactgcgga cgaactggcg caggtctggg
     1561 acgcgatagc ggcgtcgctc gatattctcg ggggctctcc gggctaagac cgccctctcg
     1621 atcctccctg tggcaaagcc atggggaggg ggaccgcgcg cgaagcgcgt ggtggagggg
     1681 ctgcgacggt gcgccatagg cccctccgtc agtgctacgc actgccacct ccccatcgct
     1741 tcgcgacagg gaggaactga ggacagctca ccgcctcaaa gccaatccaa aaaaaacggg
     1801 gcgagccaaa aggcccgccc cgcatatctt gtctgccacc caggcttaca tgccatccag
     1861 gcccttgctg accaggatgt tcaccgcgac gcggcggttc tgcgccttgc cttcgggggt
     1921 ggtgttgtcc gccgccgggt cggcttcggc catgccggtc gggctcagca tgcgataggg
     1981 cttccagccg caggcctgct gcagatggtt gaccacgcgg ccggcgcgct tttcgctgag
     2041 ctcctggttg agctcctggc tgccggtgga atcggtgtag ccgacgacga gcagcagcgc
     2101 gttgtccatg ccctcggcct ggttcgccgc gttgcaaaga tcggccttcg cctgttcggt
     2161 cagcaccgcc ttgccggtgt cgaagttcac gttggtcgtg gccttgacat tatactggtc
     2221 gatgtcgccg acccggccgc gcagggcctc ggtcgccgcc gtctgttcgg cgaagcgctg
     2281 gtcggtaccg gtgtggatca tcgacgcggt gcgcagatcc ttgctcttga ggctgacctc
     2341 gttcgcgacc aagccgccgt cccactgcag ggtgtcgacg ctgaccggaa ggccgttgag
     2401 cagcgaattc gccgcgagct tgtcgcggtc gaggccgagg aagccgccgc tgctcttgat
     2461 cttggtatat tcggcgatcg agatcgccgt gttgttgccg tccgcggtcg taacctggat
     2521 ccggccgtcg ctgcgtgccg agatgatgcc ttcgaccttg ggtcccttgg tcatctggtc
     2581 gggcgaaggc agctgaccgt aaacggtcgt catgacttcg ctatcggacg tctgttgtgc
     2641 ttgctccggc gcctgctctt gcgcctgcgc cgtcaggctg cccgtcgccg gtgcggcgag
     2701 gatcgccagc atcagcatcg cgcctgaact tttcgaaacg gcacccatac cctactcctt
     2761 caattgaacc agctgccccc gcagctcggg cccgcccctt gacgtggccc tttctgggac
     2821 atgaatactc gtgggctggt cgaaagttgc gcaggtcgtc gagcgtgatg cacggcaggc
     2881 cgataaggcc gcgtcgcaca atcgaaattt tttcattcgc ggaccggaac cggggaacga
     2941 aaggtcgctc cccggccctg acggccttca ctcgccgaat ttggcatcat cgggcatcgc
     3001 cgcccaattc ttctccgccg ccgcgataaa gcccgcgcgg tccttgtccc acttcgcctt
     3061 cacctcctga ttgaagttca ggtaaagctt gccgtcgacg atcgcatagg cgagcggatc
     3121 gccgggcgcg aggtagccgc gcgacatcgc ccacgcgcaa tggccgccat attgcggcac
     3181 gaacgccgcc ggatcggcgc ggaatttcgc cgcatgttcg gcgctcgcga aatgatagac
     3241 cgccccgtca tgctcgaccg caaaggccgc gtcgcccttc acgggcacgc cgccctcttc
     3301 gaaatagctc accgcatcat atccgctcac cgcgatgttg ccgccgtcaa caccgacata
     3361 gaccggcttc gcataggcct gcggcgtgaa ggccgcgacc gacagcggcg caagcaccgc
     3421 ggcgagcagc gcgaaaaggg cttggttttt cataagcaat ctccatctgg aaggcatcgg
     3481 ccccgcgccg gcccccgtcg ccacccagtt cgcgccgaaa gcgcaaaggg ttacggcaat
     3541 tatgcagggg gcagttcggc ttaggttcat ctgcggcgcg tcatctgcga tgcggggacc
     3601 ggggtctggg aatgcgattg tgacgaagaa gaaagccggc ctcctccccc tcctcgcggc
     3661 gctcgcgctc gccgcgccgg cgctcgcgca agaggatgac ggcagcctcg cgcagtcggg
     3721 cacgccgcca gagcgcacct cggtcctcta tacctatggc gacgaaccct gccccgaagc
     3781 gcaggagggc gagatcgtcg tctgcgcgca gcggcccgaa agcgaacgct accgcgtgcc
     3841 caagga
//
LOCUS       DATMSU010000207        13152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_145615,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000207 DATMSU010000000
VERSION     DATMSU010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 13152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13152
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145615"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VN034_05275"
     CDS             complement(<1..652)
                     /locus_tag="VN034_05275"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HWV60041.1"
                     /translation="MRDIQNIVILTGAGVSAESGIDTFRDSGGLWEQHRVEDVATPEA
                     FARDPDLVLRFYDMRREAIQTKAPNAAHEALARLDAAWPGELLIVTQNVDDLHERAGA
                     KRLIHMHGEHLNAWCTACDARVPWTGPLLDRPACSACGIVGTLRPDIVWFGEMPYRME
                     DIYHALNRADLFVSIGTSGAVYPAAGFVREARASGARTLELNMEPSQGSYWFNEARQ"
     gene            779..1507
                     /gene="dapB"
                     /locus_tag="VN034_05280"
     CDS             779..1507
                     /gene="dapB"
                     /locus_tag="VN034_05280"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWV60042.1"
                     /translation="MTSIGIIGSEGRMGVALAAAISEAGQRSCGVDRGGNVLRLASDA
                     DVLVDFSSPAALEATLDACVAAKTPIVIGTTGLEERHHYLIDDAAKDIAVLQTGNTSL
                     GVTLLAHLVREAATRLGADWDIEVVEMHHRMKVDAPSGTALLLGQAAAQGRGINLDTC
                     SERGRDGLTGARGHGKIGFASLRGGTVAGDHDVIFAGPEEMITLSHRAENRMIFARGA
                     VRAALWLLQQKPDRYTMPQVLGLN"
     gene            1512..2168
                     /gene="nth"
                     /locus_tag="VN034_05285"
     CDS             1512..2168
                     /gene="nth"
                     /locus_tag="VN034_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWV60043.1"
                     /translation="MKKADIFEFYRRLAELNPSPETELEFGNTYQLLVAVVLSAQATD
                     VGVNKATRRLFQEVKTPQQMIDLGEEGLKQHIKTIGLFNGKAKNVIALSEILVREFHG
                     EVPADRDTLVELPGVGRKTANVVMNCAFGAETFAVDTHIFRVGNRTGLAPGKTVLAVE
                     KQLEKNTPAPFRVGAHHWLILHGRYICKARTPECWRCPVVDLCRYKPKTPAPKAKKAA
                     "
     gene            2268..3641
                     /locus_tag="VN034_05290"
     CDS             2268..3641
                     /locus_tag="VN034_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWV60044.1"
                     /translation="MRNLTAPLALAAALLAAAPVHAKPADTEAAKKILKDSVAIPTVE
                     GRGQVPKLAAYYASVLKAAGYADSDIEITPIGETATFAATLHGTTKQKPIVLLGHMDV
                     VEADPKDWTRDPFVPVEEEGYIFGRGSEDNKFDVSMMVATMAQLKKDGFKPKRSIILL
                     LSGDEETSMTTTRALAAKYKGAEFALNGDGGGGTIGEDGKPKYYGLQAGEKTYADFTL
                     EVTNPGGHSSRPSGTNAIVQLSAALEKVGAYRFAPQQNELTKVGMPIVADQVGGEIGA
                     ALKAFAANPADAAAIAKIRAEPEYVGQIGTTCVPTLVKGGHAENALPQRATANINCRI
                     FPGVPVETVRAELEKIIADPAVKVNTDPDASASDASPLRPDVMAAVKKAVHARAPGLP
                     IIPSMSAGATDSYHFRAQGVPSYGVAGLFSKASDSFAHGLNERVPVDAIAPALAHWDS
                     LLRDLSK"
     gene            complement(3691..4314)
                     /locus_tag="VN034_05295"
     CDS             complement(3691..4314)
                     /locus_tag="VN034_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HWV60045.1"
                     /translation="MRPIRQAFTRESADARRADLIEATAACLAEHGLAGTNVRAISRK
                     AGVSPGLLRHYFGGIDHLVAATYQATSDRMDAIFAAAVESAGDDPRARLAAYLTASFR
                     PPVTDPELLGAWTAFWALSRNDPRMAEIHAESYAGYRARLGELLTLCGAADAGRLAIM
                     LTAMVDGLWLELSLDPESFGAEAAAQMVERAVGALLPEPPSPSSNFA"
     gene            4348..5616
                     /locus_tag="VN034_05300"
     CDS             4348..5616
                     /locus_tag="VN034_05300"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWV60046.1"
                     /translation="MATAPLRDSNADPLDGWSLPAWTYSDPDFYAAEIDRIFRPSWQV
                     VCHDSDIPNPGDWHSIDYCGESVILVRGTDRIVRAFTNVCRHRGSRLVDGAIGCAKKL
                     VCPYHAWTYELDGRLTGVPDSASYPTLDKGKAGLVPVGLEQWRGFWFVRLEDDGGPPV
                     ASMMSPYEAMIEPYRFEELGALGRVTLRPREVNWKNVGDNYSDGLHISVAHPGLTRLF
                     GKSYGVEAEERVDRMWGDLIDRPSVNWSERMYQRLLPPVPHLPENRQRHWLYFKLWPN
                     VAFDIYPDQVDFMQWLPTGPASCLIREISYVLPDERREMKASRYLNWRINRQVNAEDT
                     ALITRVQQGMQSKSFTMGPLSDKEVCLKHFCSRMRDLIPEARQEHVPPPGWSRDKSSS
                     PSGEGLGVGAIGLAPSSRAPTPTPPLKGRG"
     gene            5617..6828
                     /locus_tag="VN034_05305"
     CDS             5617..6828
                     /locus_tag="VN034_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV60047.1"
                     /translation="MLPVIIVGGGPAGMVAGLLLARAGVPVTILEKHADFLRDFRGDT
                     VHPSTLELFHEIGLLDALLKEPHAAIDTMTLNLLGQQYTIATMKHLPVAARFVAMMPQ
                     WDLLDFIAGQGRRYPTFDLRMSTEATGLTYDDAGRVHGVTLASGETLPARLVIAADGR
                     RSILRDAAELPLEDLGAPMDVLWFRIPVPAGMDMSEVALGTIDKGGMVVAIPRGDYWQ
                     CARIIEKGGFAPIEARGIAAFRDEIVAIAPGLAAGIAAIESFDDVKLLSVALDRLTRW
                     SRPGLLAIGDAAHAMSPVGGVGINLAVQDAVAAANILAAPLAAGADPDPLLVKVQERR
                     WTPTVRMQGLQRFAHRNIIEPMLRGEIARVPLAVRLLDRIPLLRRIPGRILGLGFGRQ
                     HVESPLAKEFS"
     gene            6825..8444
                     /locus_tag="VN034_05310"
     CDS             6825..8444
                     /locus_tag="VN034_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV60048.1"
                     /translation="MTKAYDALIIGAGHNGLVCAFYLAKAGLKVRIVEARDVVGGAAV
                     TEEFAPGFRNSVASYTVSLLQPKVIADMKLADHGYRVIERPISNFLPQEDGGYLKLGG
                     GLERTQAEFRKFSARDAEVLPAYYDALENVAELLRDLALRVPPNVGEGLRTLLDGARQ
                     GRRFATLSLEQQRDVLDLFTKSARTMLDSWFESEAVKAAFGFDAVVGNYASPDTPGSA
                     YVLLHHVFGEVNGKKGAWGHSVGGMGKITEIMAKVCRDMGVEISLESPVAKVLVDGGK
                     AVGVKLVGGEEIAAARVIANVGPKLLYERMMDASDLPADFQRRIKGFKAGSGTFRMNV
                     ALSELPKFTCLPHNEKDGLGEHHQSGIILAPTLDYMDRAFLDAKAHGWSKAPIVEMLI
                     PSTVDDSLAPPGQHVASLFCQQFAPELPAGRDWDDEEEAAADCIIDTVEKHAPGFRAS
                     IIAQTRLSPKGLERKFGLVGGDIMHGNMSLDQLWAARPVLGNGGYRGPVKSLYMCGAG
                     THPGGGVTGAPGHNAAQVILRDRGFFAPRWR"
     gene            complement(8406..9716)
                     /locus_tag="VN034_05315"
     CDS             complement(8406..9716)
                     /locus_tag="VN034_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWV60049.1"
                     /translation="MSDFSTGSERPGRKLGLSMCIALVMGNMIGSGVFLLPASLAPFG
                     WNGVVGWAITIAGALALAFVIARLTVAFPAASGPTGFVERAFGRVPSFMIGWAYWVSV
                     WTANVTLAVASVSFLSLFVPAIGDHMAISTIALIWLVTAINWQGARAAGRFQIVTLAI
                     KLIPLVTIVVLIPIAFGRSEPVAITPFPAEGLSFAAVSGSAILTLWALLGFESASVAA
                     DKVANPTVTIPRATIIGTLATGLLYLTVCSAIALMLPAAAVATSEAPFALFAETYWGH
                     GPAVWIAAFAAVSALGALNGWTLIQAEQPARLAEQGLLPAWFAKTNRHGTPAAALLLS
                     SGIATVCVILNSSKSTAEMFTFMAILSTSVTLWLYLACAAAALRLRVAIPVALAGLAY
                     AIWTLWGAGIGVSAMSLILMVAGLPLYIWTRLSAPAGGEEAPVA"
     gene            complement(9721..12168)
                     /locus_tag="VN034_05320"
     CDS             complement(9721..12168)
                     /locus_tag="VN034_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60050.1"
                     /translation="MRKLSTVFTTSGAFLLGSTILCSSGTALAQQANTNDDRDIVVTA
                     SKREQNLQDVPLAITAIGTERLDELQVKEFQDVVKFLPSVTIQTAAPGFSQIYFRGVA
                     SGENANHSASLPTVGTYLDEMPITTIQGALDIHAYDLARVEALAGPQGTLYGASSMAG
                     TIKLVTNQPDTSGTYGEVGVELNNVAHGDFGGVVEGFINASLGERAALRLVGWYRHDA
                     GYIDNIPGSRTYPINIPTDTDGDDENDTLVNAPGDDITQTNAGLVEKNYNDADTYGAR
                     LALGIDLDDDWTLRPTIMGQIQKTNGSYAQERSNAVTGKLQTVQYNPESSKDKWLQAA
                     LTIEGKLGNWDLTVTGGHLRRKTDVESDYSDYAYFYDALYGSAVYLYDNAGDQISPNQ
                     YIEGIDRYRRNFFEARVASPADARIRFIGGIFWQRQKHNIEQHYVIDGLSDDLEVPGT
                     VDNIWLTKQIRVDRDYAAFGEVSFDITDQLTLTGGGRVYKYDNSLVGFFGYNNPGFSS
                     NPVYACQGPAVVDGSPCTNLDKRTKKTDFIHKLNLTYKFSDDALVYATWSRGFRPGGI
                     NRRGSLPPYGSDTLDNYEFGWKTSWGPVRFNGAIYQQDWNDIQLSFLGLNGLSEIRNA
                     GVARIRGIELDLGYRQNGFSINAGMSYNDAEIRRDFCAIANDAFDCTLPGADGADNAL
                     LAPKGTALPVTPKFKGNIVARYEFPVGDLGAHVQFAVNHIGKRRSDVRTLENGIKGMF
                     DAYTTADISVGVKGESWKAELFATNLFDSNGTINAGVQCLETTCGDPDGVTANGGVFY
                     DTVVRPRLIGIKVSKEF"
     gene            complement(12310..>13152)
                     /locus_tag="VN034_05325"
     CDS             complement(12310..>13152)
                     /locus_tag="VN034_05325"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWV60051.1"
                     /translation="AAVDAIIAACTPEFFAARAGAGDPGRDPIFILGMPRAGSTLIEQ
                     ILSCHSAIEGTMELPDIPALALGLGREAKQDGRRWIEALAETPHEQLADLGAAFLDRT
                     AVQRKTDKPFYIDKLPNNWLYTAFIRLILPNAKIIDARRAPLDCCFSNFRQHFAKGQA
                     FSYGLTDVGAYYRDYVRLMAHIDTVQPGRVHRVIHEALLDDPEAEVRALLAWLEQPFE
                     EACMSFHANARAVRTASSEQVRRPINRDGVGQWQPYEKWLDPLKDALGPVLAAYPAAP
                     ADFG"
BASE COUNT         2289 a         4257 c         4376 g         2230 t
ORIGIN      
        1 cctgccgggc ctcgttgaac cagtagctgc cctggctcgg ctccatattg agctcgagcg
       61 tccgcgcgcc cgacgcgcgc gcctcgcgca cgaagcccgc ggcgggatag accgcgcccg
      121 acgtgccgat cgaaacaaac aggtcggcgc ggttcagggc gtggtaaatg tcctccatcc
      181 ggtacggcat ctcgccgaac cagacgatgt cggggcgcag ggtgccgacg atgccgcacg
      241 cgctgcacgc tggccggtcg agcaacggcc ccgtccacgg cactcgcgcg tcgcatgcgg
      301 tacaccaggc attgagatgc tcgccgtgca tatggatcag ccgtttcgcg cccgcgcgct
      361 cgtgcaggtc gtcgacattc tgcgtgacga tcagcagttc gcccggccaa gcggcgtcga
      421 gccgcgcgag cgcttcatgc gcggcgttcg gcgccttggt ctggatcgcc tcgcgccgca
      481 tatcgtagaa gcgcaggaca aggtcgggat cgcgcgcaaa ggcttcgggg gtcgcgacat
      541 cctcgacgcg atgctgttcc cagaggccgc cgctgtcgcg aaaggtgtcg atgccgcttt
      601 cggccgaaac gccggcgccg gtcaggataa cgatattctg gatgtcacgc atgactcctc
      661 tctatccgct cgcatcgagc gaagtcgaga tgcccatcgg cattgcgtca ttttgatggg
      721 tgtctcgact tcgctcgaca cgagcggcta aagggagcgg caatttaggg gacggcatat
      781 gaccagcatc ggtattatcg gcagcgaagg tcggatgggc gtcgcgctcg ccgctgcgat
      841 ctccgaagcg gggcagcgca gttgtggcgt cgaccggggc ggcaatgttc tgaggctcgc
      901 cagcgacgcc gacgttcttg tcgatttctc gtcgcccgcg gcgctcgagg cgacgctcga
      961 cgcttgcgtt gcggcgaaaa cgccgatcgt catcgggacg acggggctcg aggaacgcca
     1021 tcattatctg atcgacgatg ccgcaaagga tatcgcggtg cttcagaccg gcaacacctc
     1081 gctcggcgtc acgctgctcg cgcatctggt gcgcgaggcc gcgacgcggc tcggcgccga
     1141 ctgggatatc gaggtggtcg agatgcacca ccggatgaag gtcgacgcac cgagcggcac
     1201 cgcgctgctg ctcgggcagg cggcggcgca agggcgcggg atcaacctcg acacctgctc
     1261 cgaacgcggc cgcgacgggc ttacgggcgc gcgcggtcat ggcaagatcg gcttcgcgtc
     1321 cttgcgcggc gggacggttg cgggcgacca tgacgtaatc ttcgcggggc cggaggagat
     1381 gatcaccctc tcgcaccgcg ccgaaaaccg catgatcttc gcgcgcggcg cggtgcgcgc
     1441 ggcgttgtgg ctgctccagc agaagcccga ccgctacacg atgccgcagg tgctcgggct
     1501 taactgatcc gatgaaaaag gccgatatct tcgaattcta ccgccggctc gccgagctca
     1561 accccagccc cgagaccgag ctggagttcg gcaacaccta tcaattgctt gtcgcggtcg
     1621 tgctgtcggc gcaggcgacc gacgtcgggg tgaacaaggc gacgcggcgg ctctttcagg
     1681 aggtaaagac cccgcagcaa atgatcgacc ttggcgaaga ggggctgaag cagcacatca
     1741 agacgatcgg gctgttcaac ggcaaggcga agaatgtcat cgcgctcagc gaaatcctcg
     1801 tccgcgagtt tcacggcgag gtgcctgccg accgcgacac gctcgtcgaa ctgcccgggg
     1861 tggggcgcaa gaccgccaat gtcgtgatga actgcgcctt cggcgccgaa accttcgcgg
     1921 tcgacacgca tatcttccgc gtcggcaatc gcaccgggct cgcacccggc aagaccgtgc
     1981 tcgcggtcga aaaacagctc gaaaagaata cgcccgcacc gttccgcgtc ggcgcgcatc
     2041 actggctgat cctccacggc cgctatatct gcaaggcgcg cacccctgag tgctggcgct
     2101 gcccggtcgt cgacctctgc cgctacaagc cgaagacgcc ggcaccgaag gcgaagaagg
     2161 cggcatagga atcgtcgtcc cggcgaaagc tgggaccgct gccgccctac cacaacgata
     2221 gcgatcccag cttgcgctgg gatgacagct ggttagggtc cgtttccatg cgcaacctta
     2281 ccgctccgct cgccctcgcc gccgccttgc tcgccgccgc gcccgtccac gccaagcccg
     2341 ccgataccga ggcggcgaaa aagatactga aggacagcgt tgcgattccg acggtcgagg
     2401 ggcgtgggca ggtgccgaag ctcgccgcct attatgcgag cgtgctgaaa gccgcgggct
     2461 atgccgacag cgatatcgag atcacgccga tcggcgaaac cgcgaccttc gccgcgacgc
     2521 tgcacggtac gacgaagcag aagccgatcg tgctgctcgg ccatatggac gtcgtcgagg
     2581 ccgatcccaa ggactggacg cgcgacccct tcgtgccggt cgaggaagag ggctatattt
     2641 tcgggcgcgg gtcggaagac aataagttcg acgtctcgat gatggtcgcg acgatggcgc
     2701 agctcaagaa ggacggcttc aagccgaagc gatcgatcat cctgctgctc tcgggcgacg
     2761 aggaaacctc gatgaccacg acgcgcgcgc tcgcagcgaa gtataagggt gccgaattcg
     2821 cgctcaacgg cgacggcggt ggcgggacga tcggcgagga cggcaaaccc aaatattatg
     2881 ggctgcaggc gggcgagaag acctatgccg acttcacgct cgaggtcacc aaccccggcg
     2941 gccacagctc gcgcccgtcg ggcaccaatg cgatcgtcca gctttcggcc gcgctcgaaa
     3001 aggtcggcgc ctatcgcttc gcgccgcagc agaacgaact caccaaggtc ggcatgccga
     3061 tcgtcgccga ccaggtcggc ggcgagatcg gcgcggcgtt gaaggccttc gccgccaatc
     3121 ccgccgacgc cgcggcgatc gcgaagatcc gcgccgagcc cgaatatgtc ggccagatcg
     3181 gcaccacctg cgtcccgact ttggtcaagg gcggccacgc cgagaacgcg ctgccccagc
     3241 gcgcgaccgc gaacatcaac tgccgcatct ttcccggtgt gccggtcgaa acggtgcgcg
     3301 ccgaactcga aaagatcatc gccgatcccg cggtcaaggt gaataccgat cccgacgcca
     3361 gcgccagcga cgcctcgccg ctgcgccccg acgtgatggc ggcggtgaaa aaggcggtgc
     3421 acgcccgcgc gcccggcctg ccgatcatcc cgtcgatgag cgcgggcgcg accgatagct
     3481 atcatttccg tgcgcagggc gtgccgagct atggcgtcgc ggggctcttt tcgaaagcga
     3541 gcgacagctt cgcccacggc ctcaacgaac gcgtgcccgt cgacgcgatc gctcccgcac
     3601 tcgcgcattg ggacagtttg ctgcgcgatc tgtcgaagtg actccttctc cccttggggg
     3661 agaaggatac gcagccttgc cgcgaagcgg ctaggcgaag ttggatgagg gtgagggcgg
     3721 ttcgggcagc aacgccccca ccgccctttc gaccatctgc gccgccgctt cggcgccgaa
     3781 gctttcgggg tcgagcgaca gttcgagcca cagcccgtcg accatcgcgg tcagcatgat
     3841 cgcgagccgc ccggcgtcgg ccgcgccgca cagggtgagc aactcgccaa gtcgcgcgcg
     3901 atagcccgcg taactttccg cgtggatttc ggccatacgg ggatcatttc gcgaaagcgc
     3961 ccagaaggcg gtccatgcgc cgagcagttc ggggtcggtc accggcgggc gaaagctggc
     4021 ggtcagatag gcggcgagac gagcgcgcgg atcgtcgccc gcgctttcga ctgcggccgc
     4081 aaaaatcgcg tccatccggt cgctcgtcgc ctgataggtc gcggcgacca ggtggtctat
     4141 gccgccgaaa taatggcgca gcagccccgg cgacaccccc gcctttctag agatcgcgcg
     4201 cacgttcgtt ccagcaaggc catgttcggc gaggcacgcc gcggtcgcct cgatcaggtc
     4261 cgcccggcgg gcatcggcgc tttcgcgcgt aaaggcttgg cgaatgggcc gcatattgtt
     4321 atacgattgt ataacaagga acgagccatg gcaactgcac cccttcgcga ttcgaacgcc
     4381 gacccgctcg acggctggtc gctcccagcc tggacgtaca gcgaccccga cttctacgcc
     4441 gccgagatcg accgcatctt ccgcccgagc tggcaggtcg tctgccatga cagcgacatc
     4501 ccgaaccccg gcgactggca cagcatcgat tattgcggcg agagcgtcat cctcgtccgc
     4561 ggcaccgacc gcatcgtccg cgccttcacc aacgtctgcc gccaccgcgg ctcgcgcctc
     4621 gtcgacgggg cgattggctg cgcgaaaaag ctcgtctgcc cctatcacgc ctggacctac
     4681 gaactcgacg gccggctgac cggcgtgccc gattcggcca gctacccgac gctcgacaag
     4741 ggcaaggcgg ggctcgttcc cgtcgggctc gaacaatggc gcggcttttg gttcgtccgg
     4801 ctcgaagacg acggcggtcc gccggtcgcg agcatgatgt cgccctatga ggcgatgatc
     4861 gaaccctacc gtttcgagga actcggcgcg ctcggccggg tcacgctgcg cccgcgcgag
     4921 gtcaactgga agaatgtcgg cgacaattat tcggacggcc ttcacatctc cgtcgcgcac
     4981 cccggcctga cgcgcctgtt cggcaaaagc tatggtgtcg aggccgaaga acgcgtcgac
     5041 cgcatgtggg gcgacctgat cgaccggccg tcggtgaact ggtccgagcg catgtatcag
     5101 cggctgttgc cgccggtgcc gcaccttccc gaaaaccgcc agcgccactg gctctatttc
     5161 aagctctggc ccaatgtcgc cttcgacatc tatcccgacc aggtcgactt catgcagtgg
     5221 ctgccgaccg gcccggcgag ctgcctgatc cgcgaaattt cctatgtgct gcccgatgag
     5281 cggcgtgaaa tgaaggcgtc gcgctatttg aactggcgta tcaatcgcca ggtcaacgcc
     5341 gaggacacgg cgctcatcac gcgcgtccag caagggatgc agtcgaaaag tttcaccatg
     5401 ggacccctga gcgacaagga ggtgtgcctc aagcatttct gttcgcgaat gcgcgacctg
     5461 atccccgaag cgcggcagga gcacgtcccg ccgccgggat ggagccgcga caaaagctcc
     5521 tccccttcag gggaggggtt gggggtgggg gccatcggcc ttgcgccaag ctcgcgagcc
     5581 cccaccccaa cccctcccct gaaggggagg ggctagatgc ttccagtcat catcgtcggc
     5641 ggtggtcccg ccgggatggt cgcgggcctg ctgctcgcgc gcgccggtgt gcccgtcacc
     5701 atcctcgaaa agcacgccga tttcctccgc gacttccgcg gcgacacggt gcatccctcg
     5761 acgcttgagc tgttccatga gatcgggctg ctcgatgcgc tgctcaagga gccgcacgcg
     5821 gcgatcgaca cgatgacgct caacctgctt gggcagcaat atacgatcgc gacgatgaaa
     5881 catctgccgg tcgcggcgcg cttcgtcgcg atgatgccgc aatgggatct gctcgatttc
     5941 atcgcggggc aggggcggcg ctatccgacc ttcgatctgc gcatgtcgac cgaagcgacc
     6001 gggctgacct atgacgatgc gggccgcgtc cacggcgtga cgctggcgag cggcgagacc
     6061 ctgcctgcgc gtttggtgat cgccgccgac gggcgccgct cgatactgcg cgacgccgcc
     6121 gaactgccgc tcgaggatct gggcgcgccg atggacgtgc tctggtttcg cattcccgtg
     6181 cccgcaggca tggacatgtc cgaagtcgcg ctcggcacga tcgacaaggg cggcatggtc
     6241 gtcgcgatcc cgcgcggcga ttattggcaa tgcgcgcgga tcatcgaaaa gggcggcttt
     6301 gccccgatcg aggcgcgcgg catcgccgcc ttccgcgacg agatcgtcgc gattgcgccg
     6361 ggcctcgccg cggggatcgc cgcgatcgaa agcttcgatg atgtcaaact gctttcggtc
     6421 gcgctcgacc ggctgacgcg ctggtcgcgc cccggcctgc tcgcgatcgg cgatgccgcg
     6481 catgcgatgt cgccggtcgg cggcgtcggg atcaatctcg cggtgcagga tgcggttgcc
     6541 gctgcgaaca tcctcgccgc gccgctcgcc gccggagccg atcccgatcc gctgctcgtc
     6601 aaggtgcagg agcggcgctg gacgccgacg gtgcggatgc aggggcttca gcgtttcgcg
     6661 caccggaata tcatcgaacc gatgctgcgc ggcgagattg cgcgcgtgcc gcttgccgtc
     6721 cgcctgctcg accgcatccc gctgcttcgc cgtattcccg gccgcattct cggcctcggc
     6781 ttcggccgcc agcatgtcga atccccgctt gcgaaagaat tttcatgacc aaagcttatg
     6841 acgcgctgat catcggcgcg ggacacaacg gccttgtctg cgccttctat ctcgcgaagg
     6901 cgggactgaa ggtccgtatc gtcgaggcgc gcgacgtcgt cggcggcgcc gcggtgaccg
     6961 aggaattcgc gcccggcttc cgcaactcgg tcgcgagcta cacggtcagc ctgctccagc
     7021 caaaggtgat cgccgacatg aagctcgccg atcacggcta tcgcgtgatc gagcggccga
     7081 tcagcaattt cctgccgcag gaggatggcg gctacctcaa gctcggcggc gggctcgaac
     7141 gcacgcaggc cgagtttcgc aaattctcgg cgcgcgacgc cgaggtgctg ccggcctatt
     7201 atgatgcgct cgaaaatgtc gccgaactgc ttcgcgacct cgcgctccgc gtgccgccca
     7261 atgtcggcga ggggctgcgc accttgctcg acggcgcgcg gcaggggcgg cgcttcgcga
     7321 ccctcagcct cgaacagcag cgcgacgtgc tcgacctttt caccaaatcg gcgcgcacga
     7381 tgctcgacag ctggttcgag agcgaggcgg tcaaggcggc gttcggtttc gacgccgtgg
     7441 tcggcaatta cgccagtccc gacacgccgg gcagcgccta tgtcctgctc caccacgtct
     7501 ttggcgaggt gaacggcaag aagggcgcgt ggggccacag cgtcggcggc atgggcaaga
     7561 tcaccgagat catggcgaag gtgtgccgcg atatgggcgt cgagatcagc ctcgaaagcc
     7621 ccgtcgcgaa ggtgctcgtc gacggaggca aggcggtcgg ggtgaagctc gtcggcggcg
     7681 aggaaatcgc cgccgcgcgc gtgatcgcga acgtcgggcc gaagctgctc tacgagcgga
     7741 tgatggacgc gagcgaccta cccgccgact tccagcgccg gatcaagggc ttcaaggcgg
     7801 gcagcggcac cttccgcatg aatgtcgcgc tttcggaact ccccaagttc acctgccttc
     7861 cgcataatga aaaggatggg ctcggcgagc atcaccagtc ggggatcatc ctcgcgccga
     7921 cgctcgacta tatggaccgc gccttcctcg acgcgaaggc gcatggctgg tcgaaggcgc
     7981 cgatcgtcga gatgctgatt ccctcgaccg tcgacgacag cctcgcgccg cccggacagc
     8041 atgtcgccag cctcttctgt cagcagttcg cgcccgagct gcccgctggc cgtgattggg
     8101 atgatgagga ggaagccgcc gccgactgca tcatcgacac ggtcgaaaaa catgctcccg
     8161 gtttccgcgc gagcatcatc gcgcagacgc gcctcagccc caaaggcctc gaacgcaagt
     8221 tcggcctcgt cggcggcgac atcatgcacg gcaatatgag cctcgaccag ctctgggccg
     8281 cgcgccccgt gctcggcaat ggcggttatc gcgggccggt gaagagcctt tatatgtgcg
     8341 gcgcggggac gcaccccggc ggcggcgtca ccggcgcacc ggggcacaat gccgcgcagg
     8401 tgatcctacg cgaccggggc ttcttcgccc cccgctggcg ctgacagccg cgtccagatg
     8461 tagagcggca gccccgcgac catcaggatc aggctcatcg cgctgacccc gatcccggcg
     8521 ccccacagcg tccatatcgc ataagcgagc cccgcgagcg cgaccgggat cgcgacgcgc
     8581 aaccgcaacg ccgctgcggc gcaggcgagg tagagccaca aggtcaccga ggtcgagagg
     8641 atcgccatga acgtgaacat ctcggcggtc gatttgctgc tgttgaggat cacgcagacg
     8701 gtcgcgatgc cgctcgacag cagcagcgcg gcggcggggg tgccgtggcg gttcgtcttc
     8761 gcgaaccagg cggggagcag cccctgttcg gcaagccgcg cgggctgttc ggcctggatc
     8821 agcgtccagc cgttgagcgc gccgagcgcg ctcaccgcgg caaaggcggc gatccacacc
     8881 gcggggccgt gcccccaata ggtttcggcg aacagcgcga agggcgcctc cgaggttgcg
     8941 accgcggcgg cggggagcat cagcgcgatc gccgaacaga cggtcaggta gagcaggccg
     9001 gttgcgaggg tgccgatgat cgtcgcgcgc gggatcgtga ccgtcggatt cgcgaccttg
     9061 tcggcggcga cgctcgccga ttcgaaaccg agcagcgccc ataacgtcag gatcgccgaa
     9121 ccgctgaccg cggcgaacga caggccctcg gcggggaagg gggtgatcgc cacgggctcg
     9181 ctgcgtccga aggcgatggg gatcagcacg acgatggtga cgagcgggat cagcttgatc
     9241 gcgagcgtga cgatctggaa gcgtcccgcc gcgcgcgcgc cctgccagtt gatcgcggtg
     9301 acgagccaga tcagcgcgat cgtcgagatc gccatatgat cgccgatcgc cgggacgaag
     9361 aggctgagga aggaaacgct cgcgaccgcg agcgtgacgt tcgcggtcca caccgacacc
     9421 caataagccc agccgatcat gaagctgggg acgcgcccga aggcgcgttc gacgaagcct
     9481 gtcggcccgc ttgcggcggg aaaggcgacg gtcagccgtg cgatgacgaa agcgagtgcg
     9541 agcgcccccg cgatggtgat cgcccaaccc acgacgccgt tccagccgaa cggcgcgaga
     9601 ctggcgggga gcaggaacac gcccgacccg atcatattgc ccatgacgag cgcgatgcac
     9661 atcgaaaggc cgagcttgcg gcccggcctt tcgctgccgg tcgaaaaatc gctcacccgg
     9721 tcagaattcc ttgctgacct tgatcccgat caggcgggga cgaacgaccg tgtcgtagaa
     9781 gacaccgccg ttcgcggtga ccccgtcggg atcgccgcag gtggtttcga ggcactggac
     9841 gccggcgttg atggtgccgt tgctgtcgaa caaattcgtc gcgaaaagct cggccttcca
     9901 gctttcgccc ttcaccccga cgctgatgtc ggcggtcgta taggcgtcga acatgccctt
     9961 gatgccattt tcgagcgtgc ggacgtcgct tcgccttttg ccgatatggt tgaccgcgaa
    10021 ctggacatgc gcgccgaggt cgccgacggg gaattcataa cgcgcgacga tattgccctt
    10081 gaacttcggc gtcaccggca gggcggtgcc cttcggcgcg aggagcgcgt tgtcagcccc
    10141 gtcggcgccc ggcagcgtgc agtcgaaggc gtcattggcg atcgcgcaga aatcgcggcg
    10201 tatttcggcg tcgttgtaac tcatgcccgc gttgatcgag aagccattct gccgatatcc
    10261 gaggtcgagt tcgattccgc gaatacgcgc gacgcccgcg ttgcggatct cgctgagccc
    10321 gttgaggccg aggaaggaga gctggatgtc gttccagtcc tgctggtaaa tcgcgccgtt
    10381 gaagcgcacc ggcccccagc tcgtcttcca cccgaattca taattgtcga gcgtgtccga
    10441 gccatagggc ggcagcgacc cgcgccggtt gatgccgccc gggcggaagc cgcgcgacca
    10501 ggtcgcatag acgagcgcgt cgtcgctgaa cttgtaggtc aggttcagct tgtggatgaa
    10561 gtcggtcttc ttcgtccgtt tgtcgaggtt ggtgcagggc gatccgtcga ccaccgccgg
    10621 tccctgacag gcatagacgg gattgctact gaagccgggg ttgttgtagc cgaaaaagcc
    10681 gacgaggctg ttgtcatatt tatacacgcg gccgccgccg gtcagcgtca gctggtcggt
    10741 gatgtcgaag ctgacctcgc cgaacgcggc atagtcgcgg tcgacgcgga tctgcttggt
    10801 cagccagata ttgtcgacgg tccccgggac ctcgaggtcg tctgacagcc cgtcgatgac
    10861 ataatgctgt tcgatattat gcttttgccg ctgccagaag ataccgccga tgaagcggat
    10921 gcgcgcatcg gcgggcgagg cgacgcgcgc ttcgaagaag ttgcgccgat agcggtcgat
    10981 gccctcgata tattggttcg ggctgatctg gtcgcccgca ttgtcgtaaa gatagaccgc
    11041 cgatccatag agcgcgtcat agaaataggc atagtccgaa tagtcgctct cgacatcggt
    11101 cttgcggcgg aggtgaccgc cggtgacggt caggtcccaa ttgccgagct tgccctcgat
    11161 cgtcagcgcc gcctgcagcc atttatcctt cgacgattcg gggttgtact ggacggtctg
    11221 gagcttgccg gtgaccgcgt tcgaccgttc ctgggcatag ctgccgttgg tcttctggat
    11281 ctggcccatg atcgtcgggc gcagcgtcca gtcgtcgtcg aggtcgatgc cgagcgcgag
    11341 gcgcgcgcca taggtatcgg cgtcgttgta atttttctcg accagcccgg cgttggtctg
    11401 ggtgatgtcg tcgcccgggg cgttgacgag cgtgtcattc tcgtcatcgc cgtcggtgtc
    11461 ggtcggaatg ttgatcggat aggtgcggct gcccgggata ttgtcgatat aacccgcatc
    11521 gtggcgatac cagccgacga ggcgcagcgc cgcgcgttcg ccgagcgagg cgttgatgaa
    11581 accttcgacg accccgccga aatcgccgtg cgcgacattg ttgagctcga ccccgacctc
    11641 gccataggtg ccgctggtgt cgggctggtt ggtgacgagc ttgatcgtgc ccgccatcga
    11701 actcgcgccg taaagcgtgc cctgcggccc cgcgagcgcc tcgacgcgcg caaggtcata
    11761 ggcgtggatg tcgagcgcgc cctggatcgt cgtgatcggc atttcgtcga gataggtgcc
    11821 gaccgtcggc agcgacgccg aatgattggc gttctcgccc gacgcgacgc cgcggaaata
    11881 gatctggctg aacccgggcg cggcggtctg gatcgtcacc gacggcagga atttgacgac
    11941 gtcctggaat tctttgacct gcagttcgtc gagccgttcg gtgccgatcg cggtgatcgc
    12001 gagcggcacg tcctgcagat tctgttcgcg cttcgacgcg gtcacgacga tgtcgcggtc
    12061 gtcgttcgta ttggcttgct gcgcgagcgc ggttcccgac gagcacagga tcgtgctgcc
    12121 gagcaggaaa gcgccgctgg tcgtgaatac tgtcgatagc tttcgcatga gatccccctc
    12181 aaatgacagt catgtgacgg agaatgacgc agcaccgcgc aagcgcaagc cttgttgcgc
    12241 ggcgcggcgg tcgttggtcg aaaatgtcgt cggctgttgc ccaaaggcaa cgttttgtcg
    12301 cgggcgcgct tagccgaaat cggcgggagc ggccggatag gcggcgagta cgggcccgag
    12361 cgcgtctttc agcggatcga gccatttttc atagggttgc cattggccga cgccatcgcg
    12421 gttgatcggg cggcgtacct gttcgctcga cgcggtgcgg acggcgcgcg cgttggcgtg
    12481 gaagctcatg caggcctcct cgaacggctg ctccagccaa gcgagcaatg cgcggacctc
    12541 ggcctcggga tcgtcgagca gggcctcgtg gatcacacgg tggacgcggc cgggctggac
    12601 ggtatcgata tgcgccatca gccggacata gtcgcggtaa taggccccga catcggtaag
    12661 gccgtagctg aacgcctgcc ccttggcgaa atgctgccga aaattggaaa agcagcagtc
    12721 gaggggcgcg cgacgcgcgt cgatgatctt cgcattgggc aggatcaggc gaatgaaggc
    12781 ggtgtagagc caattgttcg gcagcttgtc gatgtaaaag ggcttgtccg tcttgcgctg
    12841 cacagcggtg cgatcgagga aggcggcgcc aaggtcggca agctgttcgt gcggcgtttc
    12901 ggcgagcgct tcgatccagc gccgtccgtc ctgcttcgcc tcgcggccga gcccgagcgc
    12961 gagcgccgga atgtcgggca actccatcgt gccctcgatc gccgaatggc agctgagaat
    13021 ctgttcgatc agcgtcgatc ccgcgcgcgg catgccgagg atgaagatgg gatcgcgccc
    13081 cggatcgccc gcgccggcgc gcgcggcgaa gaattcgggg gtgcaggcgg cgatgatcgc
    13141 gtcgaccgcg gc
//
LOCUS       DATMSU010000208         5408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_69859, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000208 DATMSU010000000
VERSION     DATMSU010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69859"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VN034_05330"
     CDS             <1..181
                     /locus_tag="VN034_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HWV60052.1"
                     /translation="PVAMAASWGFMLPSGTGPNALAWATGHIALPRILKAGLALDIIG
                     VPLLIGVIWAIAMIG"
     gene            249..1541
                     /locus_tag="VN034_05335"
     CDS             249..1541
                     /locus_tag="VN034_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HWV60053.1"
                     /translation="MAMRGKPKTNSKKIGDKELNPATLMMGLGYDPVLSEGSLKPPIF
                     LTSTFAFESAAAGKRFFEHITGKREGPADGLVYSRFNGPNQEILEDRLAVWDGADDSL
                     VFSSGMSAIATLLLALVGQNDVIVHSGPLYAATETLIARILSRFGVSFVDFPAGATRE
                     EIDAILARAQALADEKGGKVALVYLESPANPTNALVDVEAVRAARDAAFTGEAKPAIA
                     IDNTFLGPLWQKPLKQGAELVVYSLTKYAGGHSDLVAGGVSGDQRLLNIIRPMRNTIG
                     TICDPNTAWMLLRSLETLELRMSRAGENALKVCSFLRDHPKVEGLGYLGFIQDARQKD
                     IFDRHCTGAGSTFSLFIKGGEEESFRFLDALKIAKLAVSLGGTETLASHPAAMTHLSV
                     PDERKRALGITDNLVRVSIGIEDADDLIADFAQALDAV"
     gene            complement(1538..2263)
                     /locus_tag="VN034_05340"
     CDS             complement(1538..2263)
                     /locus_tag="VN034_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWV60054.1"
                     /translation="MDDPLVTAIPDYAHRVAVYDMDRTITRSGTYSGFLIHVARRRQQ
                     WRLLLLPLVGLAGAAWSLRLIDRSRLKAINLRLLVGKRFRRAEIAPLAESYADKVVAR
                     GLHPAALDQIAADREAGYRLLLATASFHLYVDAIARRLGIDDVLATRLDEPDGADHIH
                     ARLAGDNCYGDAKFARISDWLAANDITREAAHVRAYSDHVSDHPMLYFADEAVATTPS
                     RGLKLLAPKMGWQVVDWRAKRGR"
     gene            2365..3471
                     /locus_tag="VN034_05345"
     CDS             2365..3471
                     /locus_tag="VN034_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV60055.1"
                     /translation="MVARADFEHSDGADGAEVRFTGRLTLARLGDVPARLDALGAIAT
                     LDLSAIDRIDTVGAWIVTRTAKEHGSKVVGASAEAERLLDALASDKSEYRVHRDRRPA
                     WMRMLEQVGTASVAIWHELLGIIGFFGAMIVALVTQIRARRRLRWNAIVTRFQTVGVD
                     ALPIIGLMSFLIGIVIAQQGAVQLEQFGLEVFTINLVGRASIRELGLLMTAIMVAGRS
                     GSAFAAQIGTMKLTEEVDAMRTIGVSPMEAIVLPRVAASTITMPLLGFYASLCAIAGG
                     GLFCWVGLDIPPLTYIQRLREIIPMTDFWIMLIKAPVFGVLIGVTGCYEGMQVRQNAE
                     EVGQRTTSAVVAAIFLVIVLDAMFAVFFSSLGWN"
     gene            3471..4325
                     /locus_tag="VN034_05350"
     CDS             3471..4325
                     /locus_tag="VN034_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV60056.1"
                     /translation="MTEETNIEIQEELAAADAVRPEKFERAICIRGLKNQFGDAVIHD
                     GLDLDVRSGEILGVVGGSGTGKSVLMRSIIGLQTPTAGEIEVYGKTLDTIADEEESRD
                     LRRRWGVMFQGGALFSTLSVAENIQVPLREYYPRLDTNLLDEIAAYKVEMVGLPPDAG
                     PKYPAELSGGMVKRAGLARALALDPALLFLDEPTAGLDPIGAAKFDELIRELADTMGL
                     TVFLITHDLDTLYATCDRVAVLAEKKVIAVGTIPELLATEHPWIQDYFNGPRGRAAAG
                     GNQTARAR"
     gene            4335..5276
                     /locus_tag="VN034_05355"
     CDS             4335..5276
                     /locus_tag="VN034_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWV60057.1"
                     /translation="METRSNNVLVGAFVLLFTAALAVFVVWLANDSGGTKREYDIFFK
                     QSVDGLNKGAQVQFSGVPSGQVREIALWPDDPQFVRVRIEVNEGVPVLQGTTAALEGV
                     GFTGVSQISLDGAVKDAPPITDKGPAGKPVIPTRVGGLGELLNTAPQLLQRLSTLSER
                     LAELVDDENQASMRGILKNVEASTAILARNGPALEKALADTRIAIQQTGLAAEQIGNL
                     AAATQGTIDRNVDPAMRNLRETLASANESMKTLEGAIADARPGLKTFSETTIPETNAL
                     IRDLRRTSASLSSLTDKLDQQGAGALLGGSKLPDYKE"
BASE COUNT          974 a         1837 c         1705 g          892 t
ORIGIN      
        1 ccccgtcgcg atggcggcga gctggggctt catgctgccg tcgggtaccg gccccaacgc
       61 gctcgcctgg gcgaccggcc atatcgcgct gccgcgcata ttgaaggcag ggctcgcgct
      121 cgacatcatc ggcgttccgc tactgatcgg agtgatctgg gccatcgcca tgatcggatg
      181 acccgcgcaa tatagtttca aacgaaactt ggctcgctat aagggctgcc agattcaagg
      241 agcatcccat ggccatgcgc ggcaaaccca agacgaacag caagaaaatc ggtgacaagg
      301 aactgaaccc cgcaacgctg atgatggggc tcggctatga cccggtgctg tccgaaggtt
      361 cgttgaagcc gccaatcttc ctcacctcga ccttcgcctt cgaaagcgcc gcggcgggca
      421 agcgtttctt cgagcatatc accggcaagc gcgaaggccc cgccgacggc ctcgtctatt
      481 cgcgcttcaa cggcccgaat caggaaatcc tcgaagaccg cctcgcggtg tgggacggcg
      541 ccgacgacag cctcgtcttt tcgagcggca tgtcggcgat cgccaccctc ctcctcgcgc
      601 tcgtcggcca gaatgacgtg atcgtccatt cgggcccgct ttatgccgcg accgaaacgc
      661 tgatcgcgcg catcctgtcg cgcttcgggg tcagcttcgt cgacttcccc gccggcgcga
      721 cacgcgagga aatcgacgct attctggcgc gcgcgcaggc gctcgccgac gaaaagggcg
      781 gcaaggtcgc gctcgtctat ctcgaaagcc cggcgaaccc gaccaacgcg ctcgtcgatg
      841 tcgaggcggt ccgcgccgcg cgcgacgccg cctttaccgg cgaggcgaag ccagcgatcg
      901 cgatcgacaa caccttcctc ggcccgctgt ggcaaaagcc gctgaagcag ggcgccgagc
      961 tcgtcgtcta cagcctgacc aaatatgccg gcggccactc ggatctcgtc gcgggcggcg
     1021 tgtcgggcga ccagcgcctg ctcaacatca tccgcccgat gcgcaacacg atcggcacga
     1081 tctgcgatcc caataccgcg tggatgctgc tgcgcagcct cgaaacgctc gaactccgga
     1141 tgagccgcgc gggcgaaaat gcgctgaagg tctgctcttt cctccgcgat catccgaagg
     1201 tcgaggggct cggctatctc ggcttcattc aggatgcgcg gcagaaggat attttcgacc
     1261 gccattgcac cggcgccgga tcgaccttct cgctcttcat caagggcggc gaggaagaga
     1321 gcttccgctt cctcgacgcg ctcaagatcg cgaagctcgc ggtcagcctc ggcggcaccg
     1381 agacgctcgc gagccacccc gcggcgatga cgcacctgtc ggtccccgac gaacgcaagc
     1441 gggcgctcgg catcaccgac aatctcgtgc gcgtgtcgat cgggatcgag gatgccgacg
     1501 acctgatcgc cgacttcgcg caggcgctcg acgcggtcta acgaccgcgc ttggcgcgcc
     1561 agtcgacgac ctgccagccc attttcggcg cgagcagttt cagccctcgc gacggcgtcg
     1621 tagcgaccgc ttcgtcggcg aaatacagca tcggatggtc cgagacatgg tccgaatagg
     1681 cgcgcacatg cgccgcctcg cgcgtgatgt cattcgcggc gagccagtcg gaaatgcgcg
     1741 cgaacttggc gtcgccatag caattgtcgc cggcaaggcg cgcgtggata tggtcggcgc
     1801 cgtccggctc gtcgagccgc gtcgcgagca catcgtcgat ccccagccgc cgcgcgatcg
     1861 cgtcgacata gaggtggaag gaggctgtcg cgagcagcag ccggtatccc gcctcgcggt
     1921 cggcggcgat ctggtcgagt gccgccggat gcaatcccct cgccaccacc ttatcggcat
     1981 agctttcggc gagcggcgcg atctcggcgc ggcgaaaacg ctttccgacg agtaaacgaa
     2041 gattgatcgc cttcagccgc gaccggtcga tcagccgcag cgaccaggcc gcgcccgcca
     2101 gcccgacgag cggcaaaagc aagagccgcc attgctgccg ccgccgcgca acatggatga
     2161 ggaatccgct gtaagtccct gaacgcgtta tcgtccggtc catgtcgtac acggcgacgc
     2221 gatgcgcata atcgggaatg gcggtgacaa gcggatcgtc cattcccgtt ccttaatcgg
     2281 acgacgcagc accgccaagg cttggcgaac acaccatgga ttcgcttgcc gttaccgggc
     2341 aaataataca atgtcgcccg cacgatggtg gccagggcgg atttcgaaca tagcgatggt
     2401 gcggatggcg ccgaagtccg cttcacaggc cgactgacgc tcgcccgcct gggcgacgtg
     2461 ccggcgcggc tcgacgcgct cggcgcgatc gcgacgctgg acctgtcggc aatcgaccgg
     2521 atcgacacgg tcggcgcgtg gatcgtgacg cgcaccgcca aggaacatgg ctcgaaggtc
     2581 gtcggcgcga gcgccgaggc cgagcgcctg ctcgacgcgc tcgccagcga caagagcgaa
     2641 tatcgcgtcc accgcgaccg ccgcccggca tggatgcgga tgctcgaaca ggtcggcacc
     2701 gcgagcgtcg cgatctggca cgaactgctc ggcatcatcg gctttttcgg cgcgatgatc
     2761 gtcgcgctcg tcacccagat tcgcgcgcgc cgccgcctac gctggaacgc gatcgtcacc
     2821 cgctttcaga ccgtcggcgt cgacgcgctt ccgatcatcg gcctgatgag tttcctgatc
     2881 ggcatcgtca tcgcgcagca gggcgcggtg cagctcgaac aattcgggct cgaggtgttc
     2941 acgatcaatc tcgtcggccg cgcctcgatc cgcgaactcg gcctcttgat gaccgcgatc
     3001 atggtcgcgg gccgctcggg atcggccttt gccgcgcaga tcgggacgat gaaactgacc
     3061 gaagaggtcg atgcgatgcg caccatcggt gtgtcgccga tggaagcgat cgtgctgccg
     3121 cgcgtcgccg cgtcgacgat caccatgccc ctgctcggct tttacgccag tctctgcgcg
     3181 atcgcgggcg gcggcctctt ttgctgggtc gggctcgaca ttccgccgct cacttacatt
     3241 cagcgcctgc gcgaaatcat tccgatgacc gatttctgga tcatgctgat caaggcgccc
     3301 gtgttcggcg tcctgatcgg cgtcaccgga tgttacgaag ggatgcaggt gcgccagaac
     3361 gccgaagagg tcggacagcg cacaacctcc gccgttgtcg ccgcgatctt cctcgtcatc
     3421 gtcctcgacg cgatgttcgc cgtattcttc tcgtcgctcg ggtggaattg atgaccgaag
     3481 agacgaacat cgagatacag gaagagctgg cggccgccga cgccgtgcga cccgagaagt
     3541 tcgagcgcgc catctgtatc cgcggcctca agaaccagtt cggcgacgcg gtgatccacg
     3601 acgggctcga tctcgacgtg cgctcgggcg agattctcgg cgtcgtcggc gggtcgggca
     3661 ccggcaaatc ggtgctgatg cgctcgatca tcggattgca gacgccgacg gcgggcgaga
     3721 tcgaagtcta tggcaaaacg ctcgacacga tcgccgacga agaggaatcg cgcgacctgc
     3781 ggcggcgctg gggcgtgatg ttccagggcg gcgcgctgtt ttcgacgctc agcgtcgccg
     3841 aaaatatcca ggttccgctg cgcgaatatt atccgcggct cgacacgaat ctgctcgacg
     3901 agatcgccgc ctataaggtc gagatggtcg gcctgccgcc cgatgccggc cccaaatatc
     3961 cggctgagct gtcgggcggg atggtcaagc gcgccggcct cgcacgcgcg ctcgcgctcg
     4021 acccggcgct gctcttcctc gacgagccga ccgcgggcct cgacccgatc ggcgccgcca
     4081 agttcgatga gctgatccgc gaactcgccg atacgatggg cctcaccgtc ttcctcatca
     4141 cccacgatct cgacacgctt tatgcgacct gcgaccgcgt cgcggtactc gccgaaaaga
     4201 aggtgatcgc ggtcggcacc atccccgaac tgcttgccac cgagcatccg tggatacagg
     4261 attatttcaa cgggccgcgc ggccgtgctg cggcgggcgg gaaccaaacc gcgcgcgcgc
     4321 gataggaaca gctgatggaa acacgctcga acaacgtcct cgtcggcgcc ttcgtcctcc
     4381 ttttcaccgc ggcgctcgcg gtgttcgtcg tctggctcgc caacgacagc ggcggcacga
     4441 agcgcgaata tgacatattt ttcaaacagt cggtcgacgg actgaacaag ggcgcgcagg
     4501 tgcaattctc cggcgtgccg tcgggacagg tgcgcgagat cgcgctctgg cccgacgatc
     4561 cgcagttcgt ccgcgtccgg atcgaagtga acgagggtgt gccggtgctg cagggtacga
     4621 ccgccgcgct cgaaggcgtc ggcttcaccg gcgtgtcgca gatctcgctc gacggcgcgg
     4681 tcaaggacgc gccgccgatc accgacaagg ggcccgccgg caagcctgta atcccgacgc
     4741 gcgtcggcgg gctcggcgaa ctgctcaaca ccgcgccgca gctgctccag cgcctgtcga
     4801 cgctcagcga gcgcctcgcc gaactcgtcg atgatgagaa ccaggcgagc atgcgtggca
     4861 tcctcaaaaa tgtcgaagcg tcgaccgcaa tcctcgcccg caacggccca gcactcgaaa
     4921 aggcgctcgc cgatactcgc atcgcgatcc agcagaccgg cctcgcggcc gagcagatcg
     4981 gcaatctcgc cgcagcgacg caggggacga tcgaccgcaa cgtcgacccg gcgatgcgca
     5041 acctgcgcga aacgctcgcc tccgcgaacg aatcgatgaa gacgctcgag ggtgcgatcg
     5101 ccgatgcgcg gcccggcctc aagacgttca gcgaaacgac gattcccgaa accaacgcgc
     5161 tgatccgcga cctgcgccgc acttccgcct cgctgtcgag cctgaccgac aagctcgacc
     5221 agcagggcgc cggcgcgctg ctcggcggca gcaagcttcc cgattacaag gaatgaggat
     5281 gcccatgatg cgtagatttc gcgccccgct cattgccgcc atcgcggccg cgacgctgtc
     5341 gggctgcatc gggctgggcg gcaagacgcc cccctttctg ctgacgctcg accccgaggc
     5401 cgagccgc
//
LOCUS       DATMSU010000209         8828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_126970,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000209 DATMSU010000000
VERSION     DATMSU010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126970"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="VN034_05360"
     CDS             complement(<1..1458)
                     /locus_tag="VN034_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWV60058.1"
                     /translation="MFKKILIANRGEIACRVIKTARRMGIATVAVYSDADARAPFVQM
                     ADEAVHIGPSPASESYLIADKIIAACKATGAEAVHPGYGFLSERTSFAEALAKENIAF
                     IGPPVGAIAAMGDKIESKKLAKEAGVNVVPGFVGEIRDTEHAVEISNEIGYPVMMKAS
                     AGGGGKGMRLAYDEKDVREGFEATKREGLNSFGDDRVFIEKFILNPRHIEIQILGDQH
                     GNTLYLNERECSIQRRHQKVVEEAPSPFVTEKMRKAMGEQCVALSKAVGYYSAGTVEL
                     IVSGADPTGESFYFLEMNTRLQVEHPVTEAITGIDLVEQMIRVAAGEKLEMTQDDIKI
                     DGWAIENRVYAEDPYRGFLPSTGRLVRYRTPVPAWEGDERGVDGVRVDAGVEEGGEVS
                     IFYDPMIAKLVTWGATRDEAADLQVAALDRFELEGLGHNIDFVSAIMQHPRFRSGELT
                     TGFIAEEYPEGFHGADVGEDVVRALAAIAGFMASAE"
     gene            complement(1464..1856)
                     /locus_tag="VN034_05365"
     CDS             complement(1464..1856)
                     /locus_tag="VN034_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HWV60059.1"
                     /translation="MILATLMTLAAAAQEPELDCDNAMAQFELNACAYKEYERADAAM
                     NAQWKVTAARMKEIDADFDRSQDNRPGYFDTLLAAQRAWLTYREQHCAGEGYTMRGGS
                     AEPMVISGCQTQLTEARTKQLKDLIEEY"
     gene            complement(1853..2890)
                     /gene="bioB"
                     /locus_tag="VN034_05370"
     CDS             complement(1853..2890)
                     /gene="bioB"
                     /locus_tag="VN034_05370"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWV60060.1"
                     /translation="MNLSPNAEQDDLPRTDWTREEIAELFDLPFDELMWEAQGVHRRH
                     HARGEVQLCTLLSIKTGGCVEDCGYCSQSKSADSGLKATKLMDVRAVLQSAAQAKDSG
                     SKRFCMGAAWRNPKDRDMPAIVEMIEGVRQMGMETCMTLGMLTKEQAQTLAVAGLDYY
                     NHNIDTSPENYENIISTRTFQDRLDTLEEVRNAGINVCSGGIVGLGENRQDRVGFIHA
                     LATLERHPESVPVNALVPVKGTVLGDMLADTPLAKIDDIEFVRTIAVARITMPKSMVR
                     LSAGRESMSEATQALCFMAGANSIFTGDKLLTTGNRGDNADAALFAKLGIVPMVSEEP
                     MRREAELEAAE"
     gene            complement(2909..3346)
                     /locus_tag="VN034_05375"
     CDS             complement(2909..3346)
                     /locus_tag="VN034_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60061.1"
                     /translation="MISALGLMLAGLAASQTAAEAGGRESAFRPDIGVEHVYHQQLDD
                     VYFTDWFARLENSKGPWRDVYFETSDKFVNKGIIRLNCDDPEADIDLTLYSTGDYGSD
                     ADRREVTVPYADRRAWADGGYEPLAGETPPFEFYSAALTRFCK"
     gene            complement(3343..5490)
                     /gene="scpA"
                     /locus_tag="VN034_05380"
     CDS             complement(3343..5490)
                     /gene="scpA"
                     /locus_tag="VN034_05380"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HWV60062.1"
                     /translation="MTDKPTLDQWAAAADKEVKGKDLTWATPEGIDVKPLYTAEDVQT
                     DPGLPGFAPFTRGVRASMYAGRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSVAFD
                     LATHRGYDSDHPRVVGDVGKAGVAIDSVEDMKILFDGIPLDQMSVSMTMNGAVIPILA
                     FFIVAGEEQGVERKLLDGTIQNDILKEFMVRNTYIYPPEPSMRIISDIFGYTSREMPK
                     FNSISISGYHMQEAGATQVQELAFTIADGAEYVGYGVASGLDIDKFAGRLSFFFAIGM
                     NFFMEIAKLRAARVLWHRVMTNLGAKDERSKMLRTHCQTSGVSLTEQDPYNNVMRTTI
                     EAMAAMLGGTQSLHTNALDEAIALPTDFSARIARNTQIVIQEETGMTKVVDPLGGSYY
                     VEALTQELVDKAWEIIERVEKEGGMAKAVAAGWPKAMIETAAAARQARVDRGDDVIVG
                     VNKYRLKDEDLLETLEVDNTKVREAQVARINKTKASRDEGACQAALDALRKAAAAPQS
                     IETNLLAHAVEAARARATLGEISSAMEESFERYGTQPTPVKGVYAAPYEGDSRWQQVL
                     DGVKAVERRMGRKPKLLVAKMGQDGHDRGANVIASAFGDMGFDVVSGPLFQTPEETVV
                     LALDSGVDVVGASSLAAGHKTLIPELIGKLKEAGRGDIKVIAGGVIPPQDYQYLRDAG
                     VQGIYGPGSNVVECAADVLRLLGHNMPPLEDAA"
     gene            complement(5641..6078)
                     /gene="mce"
                     /locus_tag="VN034_05385"
     CDS             complement(5641..6078)
                     /gene="mce"
                     /locus_tag="VN034_05385"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWV60063.1"
                     /translation="MKLGRLNHIGVATPSIADSIVFYRDVMGATQIHTPFDLPEQGVK
                     VCFVDTPTDSGLNGTQIELIEPLPGNASIAAFLQKNPAGGQHHLCYEVPDIHAAKAEF
                     EAMGKRVLGEPRIGAHGTLIFFLHPKDMGGVLTEIMETPKATH"
     gene            complement(6089..6838)
                     /locus_tag="VN034_05390"
     CDS             complement(6089..6838)
                     /locus_tag="VN034_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60064.1"
                     /translation="MRKSVLAFTALLAGLSAPAASAFDPDTPVGETKEAFPVTLGSDE
                     DTTIGLAFQTAFGLPRGAEPEAIRTIDERAYRFRPVAIHLLPNNVGVLLSAGSLDDAG
                     HSEGGLNAIHYLKSSAAGWVKQGEWIGIGGTGTVGNAATSWAFTNLLGRNPYLITAGG
                     GVWQGCAIGSAVVTELTPDGPVDRGGFIDGMSSGAGIGQTEQEYGGRIAAAVPDKSFT
                     VAYTGTRSFKQQYVLNNGNYEPVGKDQVPGC"
     gene            complement(6933..8465)
                     /locus_tag="VN034_05395"
     CDS             complement(6933..8465)
                     /locus_tag="VN034_05395"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010214961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HWV60065.1"
                     /translation="MSANIAEMERRRAAAALGGGQKRIDAQHAKGKLTARERLDVLLD
                     EGSFEELDTYVEHNCTDFGMQDQKVPGDGVVTGSGTINGRLVYVFSQDFTVFGGSLSE
                     RHAEKIMKVMDNAMKVGAPVIGLNDSGGARIQEGVASLGGYAEVFQRNVLASGVVPQI
                     SLIMGPCAGGAVYSPAMTDFIFMVKDSSYMFVTGPDVVKTVTNEVVTQEQLGGAITHT
                     TKSSVADNAFENDIEALLAARDFFDYLPENNRSGVPVRPTSDPYDRAEASLDTLIPPN
                     ANQPYDMHELIRKTVDEGDFFEVQPAHAANIICGFGRIEGRTIGIVANQPLVLAGVLD
                     INSSKKAARFVRFCDAFEIPIITFVDVPGFLPGTAQEYNGIIKHGAKLLFAYAEATVP
                     KITVITRKAYGGAYDVMASKHLRGDLNYAWPTAEIAVMGAKGAVEIIFRSDIGDPEKI
                     AQRTKEYEDRFANPFVAASRGYIDEVIHPHNTRKRIALGLRKLRNKQLENPWKKHDNI
                     PL"
     gene            8678..>8828
                     /locus_tag="VN034_05400"
     CDS             8678..>8828
                     /locus_tag="VN034_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV60066.1"
                     /translation="MARQRIFAGTRLKQLRIERGTMQAAFAQQLGISTSYLSQIEHDD
                     RPLTPA"
BASE COUNT         1429 a         2868 c         2747 g         1784 t
ORIGIN      
        1 ctcggcgctc gccatgaagc ccgcgatcgc cgcgagcgcg cgcacgacgt cctcgccgac
       61 atcggcgccg tggaagcctt cgggatattc ctcggcgatg aagccggtcg tgagctcgcc
      121 cgaacggaag cgcgggtgct gcatgatcgc cgagacgaaa tcgatattgt ggccgagccc
      181 ttcgagttcg aaacggtcga gcgccgcgac ctgcaggtcg gcggcttcgt cgcgcgtcgc
      241 gccccaggtc accagcttgg cgatcatcgg gtcgtaaaag atcgacactt cgccgccctc
      301 ttcgaccccc gcatcgactc gcacgccgtc gacgccgcgc tcatcgcctt cccacgccgg
      361 gacgggggtg cgatagcgca cgagccgccc ggtcgatggc aggaagccgc ggtacgggtc
      421 ttcggcatag acgcggttct cgattgccca gccgtcaatc ttgatgtcgt cctgcgtcat
      481 ttcgagcttc tcgcccgcgg caacgcggat catctgctcg acgaggtcga tgccggtgat
      541 cgcctcggtc accggatgct cgacctgcag ccgcgtgttc atttcgagga agtagaagct
      601 ctcgcccgtc gggtcggcgc ccgagacgat gagttcgacc gtgcctgcgc tatagtagcc
      661 cacggccttc gacagtgcga cgcactgctc gcccatcgcc ttgcgcatct tttcggtcac
      721 gaacggcgac ggcgcttcct cgacgacctt ctggtggcgg cgctggatgc tgcattcgcg
      781 ttcgttgagg tagagcgtgt tgccgtgctg atcgccaagg atctggatct cgatgtggcg
      841 cgggttgagg atgaatttct cgatgaagac gcggtcgtcg ccgaagctgt tgagcccctc
      901 gcgcttggtc gcctcgaagc cctcgcggac gtccttttcg tcataggcaa ggcgcatgcc
      961 cttgccgccg ccgcccgccg acgccttcat catcaccgga tagccgatct cgttcgaaat
     1021 ctcgacggca tgctcggtat cgcggatctc gccgacgaag ccggggacga cgttcacgcc
     1081 cgcttccttg gcaagcttct tcgattcgat cttgtcgccc atcgccgcga tcgcgcccac
     1141 cggtgggccg atgaaggcga tattttcctt cgccagcgcc tcggcaaagc tcgtgcgctc
     1201 ggacaggaag ccatagcccg gatgcaccgc ctcggcgccc gtcgccttgc acgcggcgat
     1261 gatcttgtcg gcgatcagat aggattcgga cgcgggcgac gggccgatgt gcacggcctc
     1321 gtcggccatc tggacgaacg gcgcgcgtgc gtcggcgtcc gaatagacgg cgaccgtcgc
     1381 gatacccatg cgccgcgcgg tcttgatgac gcggcaggcg atttcaccgc ggttggcgat
     1441 caggattttc ttgaacatct ctctcaatat tcctcgatca aatctttgag ttgcttggtg
     1501 cgcgcttccg tcaactgagt ctggcagccg ctgatcacca tcggttcggc cgaaccgccg
     1561 cgcatcgtgt agccttcgcc ggcgcaatgc tgttcgcggt aggtgagcca cgcgcgctgc
     1621 gcggcgagca gcgtgtcgaa atagccgggg cggttgtcct gcgagcggtc gaaatcggcg
     1681 tcgatttcct tcatgcgcgc ggccgtcacc ttccattgcg cgttcatcgc ggcgtcggcg
     1741 cgctcatatt ccttgtaggc gcaggcgttg agttcgaact gcgccatcgc attgtcgcaa
     1801 tcgagctcgg gttcctgcgc agccgcagcc agcgtcatga gcgttgccaa tatcattccg
     1861 ccgcctccag ctcagcttcc cgccgcatcg gctcttcgct caccatcggg acaatcccca
     1921 gcttcgcgaa cagcgccgcg tcggcattgt cgccgcggtt gccggtcgtg agcagcttgt
     1981 cgccggtgaa gatgctgttc gcgcccgcca tgaagcagag tgcctgcgtc gcttccgaca
     2041 tgctttcgcg tcccgcagac aggcggacca tcgatttcgg catggttatg cgcgcgaccg
     2101 cgatggtgcg gacgaactcg atatcgtcga tcttcgcgag cggggtgtcg gcgagcatgt
     2161 cgccgagcac ggtgcccttc accggcacca gcgcattgac cggcacgctt tcggggtggc
     2221 gttcgagcgt cgcgagcgcg tggatgaagc cgacgcggtc ctgccggttc tcgcccagcc
     2281 cgacgatgcc gcccgagcag acgttgatcc cggcgttgcg gacctcctca agcgtgtcga
     2341 gccggtcctg aaaggtgcgg gtcgagatga tattctcgta attctccggg gaagtgtcga
     2401 tattatggtt gtaatagtcg agccccgcga ccgcgagcgt ctgcgcctgc tccttggtca
     2461 gcatgccgag cgtcatgcag gtttccatgc ccatctggcg cacgccctcg atcatttcga
     2521 cgatcgcggg catgtcgcgg tccttggggt tgcgccacgc cgcgcccatg cagaagcgct
     2581 tcgagcccga atccttcgcc tgcgccgccg attgcagcac tgcgcgcacg tccatcagct
     2641 tggtggcctt aagcccggaa tccgccgatt tgctctgcga gcaatagccg caatcctcga
     2701 cgcagccgcc ggtcttgatg ctgagcaagg tgcaaagctg cacctcgccg cgcgcatgat
     2761 ggcggcgatg gacgccctgc gcctcccaca tcagttcgtc gaacggcagg tcgaacagct
     2821 cggctatttc ttcgcgcgtc cagtcggtac gcggcaggtc atcctgttcg gcgttgggag
     2881 agaggttcat gttcgtcgac ccttttcgtt atttgcaaaa gcgcgtcagc gcggcgctat
     2941 agaattcgaa cggcggtgtc tcgcccgcaa gcggttcata gccgccatcg gcccaggcac
     3001 ggcgatcggc atagggcacc gtgacctcgc ggcgatcggc gtcgctgcca taatcgccgg
     3061 tgctgtaaag ggtcagatcg atatcggctt cgggatcgtc gcaattgagg cggatgatcc
     3121 ccttgttcac gaacttgtcg ctcgtctcga aatagacgtc gcgccacggc cccttggagt
     3181 tttcaagccg ggcgaaccag tcggtgaaat aaacgtcgtc gagctgctga tgatagacat
     3241 gctcgacgcc gatatccggg cgaaacgcgc tttcacggcc gccggcttcc gccgccgtct
     3301 ggctcgccgc cagccccgcc agcatcagac ccagcgcgct gatcacgcgg catcctccag
     3361 cggcggcata ttgtggccga gcaggcgcag cacgtcggcg gcgcattcga cgacgttgga
     3421 gccggggcca tagatgccct gcactcccgc gtcgcgcaga tattgatagt cctgcggcgg
     3481 gatgacgccg cccgcgatca ccttgatgtc gcccctgccc gcttccttca gcttgccgat
     3541 cagctcgggg atcagcgtct tgtgccccgc ggcgaggctc gacgcgccga cgacatcgac
     3601 ccccgaatcc agcgcgagca cgacggtttc ctcgggcgtc tggaacagcg gccccgaaac
     3661 gacgtcgaaa cccatgtcgc caaacgccga tgcgatgacg ttcgcgccgc ggtcgtgccc
     3721 gtcctgcccc attttggcga cgagcaattt gggtttccgc cccatccggc gttcgaccgc
     3781 cttcaccccg tcgagcacct gctgccagcg gctgtcgccc tcatagggtg cggcgtaaac
     3841 gcccttcacc ggggtcggct gggtgccata gcgctcgaag ctttcttcca tcgccgacga
     3901 aatttcaccg agcgtcgcgc gggcgcgcgc ggcttcgacc gcatgggcga gcaaattggt
     3961 ctcgatcgac tgcggcgccg cggccgcctt gcgcagcgca tcgagcgcgg cctggcacgc
     4021 tccttcgtcg cggctcgcct tggtcttgtt gatccgcgcg acctgcgctt cgcgcacctt
     4081 ggtgttgtcg acttcgagcg tttcgagaag atcctcgtct ttcaggcgat atttgttcac
     4141 gccgacgatc acatcgtcgc cgcggtcgac gcgcgcctga cgggcggccg ccgcggtctc
     4201 gatcatcgcc ttgggccagc cggctgcgac ggccttggcc atcccgcctt ctttctcgac
     4261 ccgctcgata atctcccacg ccttgtcgac cagctcctgc gtcagcgcct cgacataata
     4321 ggagccgccg agcgggtcga cgaccttggt catccccgtc tcttcctgga tgacgatctg
     4381 cgtgttgcgc gcgatgcgcg ccgagaaatc ggtcggcagc gcgatcgctt cgtcgagcgc
     4441 gttggtgtgc agcgactggg tgccgccgag catcgccgcc atcgcctcga tcgtcgtgcg
     4501 catgacattg ttgtacgggt cctgctcggt gagcgagacg cccgaggtct ggcagtgcgt
     4561 gcgcagcatc ttcgagcgct cgtccttggc gccgaggttc gtcatcacgc ggtgccacag
     4621 cacgcgcgcg gcgcggagct tggcgatttc catgaagaag ttcatgccga tcgcgaaaaa
     4681 gaagctgagg cgcccggcga acttgtcgat gtcgaggccg cttgcgacgc catagcccac
     4741 atattcggcg ccgtcggcga tggtgaaggc gagctcctgc acctgcgtcg cgccggcttc
     4801 ctgcatatga tagccggaga tcgagatgct gttgaacttc ggcatctcgc ggctggtgta
     4861 gccgaagatg tccgagatga tccgcatgct cggttcgggc ggatagatat aagtgttgcg
     4921 gaccatgaac tccttcagaa tgtcgttctg gatggtcccg tcgagcaatt tgcgctcgac
     4981 cccctgttcc tcgcccgcga cgatgaagaa ggcgaggatc gggatcaccg cgccgttcat
     5041 cgtcatcgat accgacatct ggtcgagcgg aatgccgtcg aacaggatct tcatgtcctc
     5101 gacgctgtcg atcgcgacgc ccgccttgcc gacgtcgccg acgacgcgtg gatggtcgct
     5161 gtcatagccg cggtgggtgg cgagatcgaa ggcgaccgac aggcccttct gtcccgcggc
     5221 gaggttgcgg cgatagaagg cgttcgattc ctcggcggtc gagaagccgg cgtactgccg
     5281 gatcgtccac ggccgccccg cgtacatcga agcgcgcacg ccgcgcgtga agggcgcgaa
     5341 accggggagg ccgggatcgg tctggacgtc ctcggccgtg taaagcggct tgacgtcgat
     5401 cccctcgggc gtcgcccagg tgaggtcctt gcccttcacc tccttgtcgg cggcggcagc
     5461 ccattggtcg agggtcggtt tgtcggtcat atgcagtctc gttctctcat tcgatctctc
     5521 aaattcgtca tgctgaactt gtttcagcat ccatggcctg agttcgaatt tcacgccgcg
     5581 tcgaaggaac cggcaggcca tggaccctga aacaagttca gggtgacgag gataaacagg
     5641 tcagtgcgtc gctttcggcg tctccataat ctcggtcagc accccgccca tatccttggg
     5701 gtgcaggaaa aagatcagcg tcccgtgcgc gccgatgcgc ggctcgccga ggacacgctt
     5761 gcccatcgcc tcgaactcgg ctttcgcggc gtggatgtcg ggcacttcgt agcacagatg
     5821 atgctggcct cctgcggggt tcttttgcag gaaagccgca atcgatgcat tgcccggcaa
     5881 tggttcgatc agctcgattt gtgtgccgtt cagcccgctg tcggtcggcg tatcgacgaa
     5941 acagaccttc accccctgct cgggcaggtc gaacggcgta tgaatctgcg tcgcgcccat
     6001 cacgtcgcgg taaaagacga tgctgtcggc gatcgacggc gttgcgacgc cgatgtggtt
     6061 gaggcggcct agtttcatct cattctcctc agcaacccgg aacctggtcc ttgccgaccg
     6121 gttcatagtt gccgttattc agcacatatt gctgcttgaa ggaccgcgtg ccggtgtagg
     6181 cgacggtaaa gctcttgtcg ggcacggccg ctgcgatccg gcctccatat tcctgttcgg
     6241 tctggccgat tccggcgccc gaactcatcc cgtcgataaa gccgccgcgg tcgaccggcc
     6301 cgtcgggcgt caactcggtc accacggccg atccgatcgc gcagccctgc cacacgccgc
     6361 cgccggcggt gatcagatag ggattgcgcc cgagaaggtt cgtgaaggcc cagctcgtcg
     6421 cggcgttgcc caccgtgccg gtgccgccga tcccgatcca ttcgccctgt ttgacccagc
     6481 ccgcagcgct cgacttgaga taatggatcg cattcagtcc gccttcgctg tgccccgcat
     6541 cgtcgagcga cccggcgctc agcagcacgc cgacattatt cggtagcagg tggatcgcga
     6601 ccgggcggaa acgataggcc cgttcgtcga tcgtccggat ggcttcgggt tcggcgccac
     6661 gcggcaggcc gaaggcggtc tggaatgcga gcccgatcgt cgtgtcctcg tcgctgccga
     6721 gcgtgaccgg aaaggcttcc ttcgtttcgc ctaccggcgt gtcggggtcg aacgccgatg
     6781 ctgccggcgc cgaaagtccg gccaggagag ctgtaaaggc aagaacggat ttacgcatct
     6841 cgatacctca tctgatagtc gaacatcgcg gccgcgtttc cgggccgcag tccctaacta
     6901 tctcatccac agcgaaattt tagtgcccgt tgtcacagcg gaatattgtc gtgcttcttc
     6961 cacgggtttt cgagttgctt gttccgtagc ttgcgtagcc ccagcgcgat ccgcttgcgc
     7021 gtgttgtgcg ggtggataac ctcgtcgata tacccccgcg acgccgcgac gaacgggttc
     7081 gcgaaacggt cctcatattc cttcgtgcgc tgcgcgatct tttcggggtc gccgatgtcg
     7141 ctgcggaaga tgatctcgac cgcgcccttc gcgcccatca ccgcgatctc ggcagtcggc
     7201 caggcgtagt tgaggtcgcc gcgcagatgc ttcgacgcca tcacgtcata ggcgccgcca
     7261 taggccttgc gcgtgatcac cgtgatcttg ggcaccgtcg cctcggcata ggcgaagagc
     7321 agcttcgcgc catgcttgat gatgccatta tactcctgcg cggtgccggg gaggaagccg
     7381 gggacgtcga cgaaggtgat gatcggaatc tcgaacgcgt cgcagaagcg gacgaagcgc
     7441 gccgccttct tcgacgaatt gatgtcgagc acgcccgcga gcaccagcgg ctggttcgcg
     7501 acgatcccga tcgtccgccc ctcgatgcgt ccgaagccgc agatgatgtt cgcggcatgc
     7561 gccggctgca cttcgaagaa atcgccttcg tcgaccgtct tccggatcag ctcgtgcatg
     7621 tcatagggct ggttcgcatt cggcgggatc agcgtgtcga ggctggcctc ggcgcggtca
     7681 taggggtcgc tggtcgggcg cacggggacg ccgctacggt tgttctcggg caggtaatcg
     7741 aagaaatcgc gcgccgcgag cagcgcttcg atgtcgttct cgaacgcatt gtcggcgacc
     7801 gagcttttcg tcgtgtgggt gatcgcgccg ccgagctgtt cctgcgtcac cacttcgttg
     7861 gtgaccgttt tgaccacgtc aggcccggtg acgaacatat agctcgagtc cttcaccatg
     7921 aagatgaagt cggtcattgc cgggctgtaa accgcgccgc ccgcgcacgg gcccatgatc
     7981 aggctgatct gcggcacgac gcccgatgcc agcacgttgc gctggaacac ctcggcatag
     8041 ccgccgagtg aagcgacgcc ttcctggatg cgcgcgccgc cgctgtcgtt gaggccgatg
     8101 acgggcgcgc cgaccttcat cgcattgtcc atgaccttca taatcttttc ggcatgccgc
     8161 tcggacagcg agccgccgaa gacggtgaaa tcttggctga agacatagac gaggcggccg
     8221 ttgatcgtgc ccgacccggt gacgacgccg tcgccgggca ctttctggtc ctgcatcccg
     8281 aaatcggtgc aattatgctc gacataggtg tcgagctcct cgaacgaacc ctcgtcgagc
     8341 agcacgtcga gccgctcgcg cgcggtcagc ttgcccttgg cgtgctgcgc atcgatgcgc
     8401 ttctgcccgc ccccaagggc tgcggcggcg cggcggcgtt ccatttcggc gatattggcg
     8461 gacaaggcgg ctctccatca gaattcgaga gcgtcgtctg cccgcttcgc atcgctgaag
     8521 caaatgtgaa tttgcaaaat tgcgaagtct gattttgcaa aatttgttgc ctgcgtatag
     8581 gctcctcatc cgtttggaca gcgtcgaggc gcaatcgatt acgtctcaaa tcgattgcac
     8641 ctcggcaccc gccgaaaatt tgaggaagga atgcccgatg gctcggcagc gcatttttgc
     8701 cggaacccgg ctgaagcagc tgcgcatcga gcgcggcacg atgcaggccg ccttcgcgca
     8761 gcagcttggc atctcgacct cctacctcag ccagatcgag catgacgacc gcccgctgac
     8821 tccggcgc
//
LOCUS       DATMSU010000210         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_13121, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000210 DATMSU010000000
VERSION     DATMSU010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13121"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VN034_05405"
     CDS             <1..420
                     /locus_tag="VN034_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HWV60067.1"
                     /translation="LQIHAARFNELFRYRWDRIVEFLKLHYALSRREEPYWLAQREAA
                     HVPPRLAEMLALWRDQPPSAWDFPRVDEIFSAESHQYILYGMGFPVPADLPTTGGRAE
                     SLLADTRQRARSLAAALPANRDYLDALRPGPHAAAMQ"
     gene            432..1151
                     /locus_tag="VN034_05410"
     CDS             432..1151
                     /locus_tag="VN034_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HWV60068.1"
                     /translation="MTRHAVLDNMTHRELRVRTDAGAELGDDVMATLTVPTEFRRVQA
                     HFPILFRRETGRDDFTALAMFGFQNGENLFLEDGRWDARYRPLSLAIGPFLVGRPAGG
                     EGAGQVHIDLDHPRIADASEGVRLFDGEGKATPYLDEIAERLGDLDEGYRESRAFYDA
                     LLAYDLLEPFSLEVTLDDGSVHSLVGFHIIDEAKLRALDGETLGALHAAGHLMPMFMA
                     LASLSQLSALVARKNRRLGGG"
     gene            1144..2175
                     /locus_tag="VN034_05415"
     CDS             1144..2175
                     /locus_tag="VN034_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HWV60069.1"
                     /translation="MAEQAGAFTDRLARVPERLWDKGAGAGALLEDAREPFVLRGLVA
                     GWPLVEAGKRSARDARRYLLDHARDRPFKVSIGPPGHDGRLFYDDEMAMNFRVGTGKL
                     ADIFAGIDTAEERGEARTVYLASIDIPSHFDGLDEANRVELGDRDPLKSIWIGTRTKI
                     AAHNDFPDNLACCAAGRRRFTLFPPGAFRDLYLGPIDNTPAGRVVSMVDFDAPDLAKH
                     PRFVDAMAEALTVELEPGDAIFIPTMWWHHVEGLADFNILINYWWRRTPAWLGQPQAA
                     LNHAILAIRDLPPEDKAIWREQFDHYVFENDDSVTDHIPETARSILAPLTSETAGRLR
                     AFLLRALSK"
     gene            2172..3671
                     /locus_tag="VN034_05420"
     CDS             2172..3671
                     /locus_tag="VN034_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HWV60070.1"
                     /translation="MSDTKSVQRIVIGGGGTAGWMMAAAIARTMGHTVDLTLVESEAI
                     GTIGVGESTIPPLVNFNRILGIPETEFMRAAQATFKLGILFDNWKHDGDSYFHSFGLS
                     GKDHWSAGFQHFWLNARERGHEQPYADYCLELVAGMQGKFAHLPEERMNYAYHVDATL
                     YGRYLRKLAEADGCKRVEGKIARVELNGESGDIAALHLDGERRIEGDMFVDCTGFRGL
                     LIDGALHAGFEDWGHWLPNDSAIAVQAKHQGPPMPYTQAIAHDAGWQWRIPLQHRMGA
                     GIVYSSGYLSRDAAYDRLMSSVGEPLIEPFDIKFRGGVRRKQWFRNCVAVGLAGGFVE
                     PLEATTVHLIQRAVLRFIRLMPNGRVSERDAMEFNDQQRLDIEQIRDFVVLHYKATNR
                     RDSPFWRHVAAMPIPDSLQQKIELFRETGRVFRKNEELFAENSWVQVMMGQGIEPQSY
                     HPIAAKLRDDEVERLFQTLRDNIEQTVATLPEHHAYVARYCGAEEPKAA"
BASE COUNT          626 a         1191 c         1238 g          633 t
ORIGIN      
        1 ctgcagatcc acgccgcgcg tttcaacgag ctgttccgct atcgctggga ccgtatcgtc
       61 gaattcctca agctgcatta cgcgctcagc cggcgcgagg aaccctattg gctggcgcag
      121 cgagaagcgg cgcatgtccc gccgcggctg gccgagatgc tggcgctctg gcgcgaccag
      181 ccgccatcgg cctgggactt cccgcgcgtc gacgagattt tctccgccga gagccatcaa
      241 tatatcctct atggcatggg ctttccggtc cccgccgatc tgccgacgac gggcgggcgt
      301 gcggaaagcc tgcttgccga cacccgccag cgcgcgcggt cgctcgccgc ggcgctgccc
      361 gccaatcgcg attatctcga cgcgctgcga cccggccccc atgccgccgc aatgcaatag
      421 gaaggaattt gatgacccgc cacgccgtcc tcgacaatat gacccatcgc gaattgcgcg
      481 tccgcaccga cgcgggggcg gaattgggcg acgatgtcat ggcgacgctg acggtgccga
      541 ccgaattccg gcgcgtgcag gcgcatttcc cgatcctgtt ccgccgcgaa acggggcgcg
      601 acgacttcac cgcgctggcg atgttcggat ttcagaatgg cgagaatctg tttctcgaag
      661 acgggcgctg ggatgcgcgc tatcgcccgc tgtcgctcgc gatcgggccg ttcctggtcg
      721 ggcgtccggc gggcggcgag ggggcggggc aggtccatat cgacctcgac catccgcgga
      781 tcgccgatgc gagcgaggga gtgcggctgt tcgacggcga agggaaggcg accccctatc
      841 tggacgagat cgccgaacgg ctcggcgacc tcgacgaggg ctatcgcgag agccgcgcct
      901 tctacgatgc gctgctggcc tacgacctgc tcgaaccctt cagcctcgag gtcacgctcg
      961 acgacggttc ggtccactcg ctcgtcggtt tccacatcat cgacgaggcg aagctgcgcg
     1021 cgctcgacgg cgagacgctc ggcgcgctgc acgcggcggg gcatctgatg ccgatgttca
     1081 tggcgctcgc ttcgctgtcg cagctgtcgg cgctcgtcgc gcgcaagaac cgcaggctcg
     1141 gcggtggctg aacaggccgg cgcctttacc gaccgcctcg cgcgggtgcc cgagcgccta
     1201 tgggataagg gtgcgggggc gggcgcgttg ctggaagacg cgcgcgagcc ctttgtcctg
     1261 cgcgggctcg tcgccggctg gccgctcgtc gaagcgggca aaaggtcggc gcgcgatgcg
     1321 cggcgttatc tgctggacca tgcgcgcgat cgcccgttca aagtgtcgat cggcccgccg
     1381 gggcacgacg gtcgcctgtt ctacgacgac gaaatggcga tgaatttccg tgtcgggacg
     1441 ggcaagctcg ccgatatttt cgccggtatc gataccgccg aggaacgcgg cgaggcacgt
     1501 accgtctatc tggcctcgat cgatattccg tcgcatttcg acgggctcga cgaagcgaac
     1561 cgggtcgagc ttggcgaccg tgatcccttg aagagcatct ggatcggcac gcggaccaag
     1621 atcgcggcgc acaacgactt ccccgacaat ctggcgtgct gcgccgccgg gcgccgccgc
     1681 ttcacgttgt tcccgccggg cgcgttccgc gatctctatc tggggccgat cgacaatacg
     1741 cccgcaggac gcgtcgtcag catggttgat ttcgacgcgc ccgatctcgc gaagcatccg
     1801 cgcttcgtcg atgccatggc cgaggcgctg acggtggagc tcgaaccggg cgacgcgatc
     1861 ttcatcccga cgatgtggtg gcaccatgtc gaggggctcg cggacttcaa tatcctcatc
     1921 aattactggt ggcgccgcac acccgcctgg ctcgggcagc cgcaggcggc gctcaaccat
     1981 gcgatccttg cgatccgcga cctgccgccc gaagacaagg cgatctggcg cgaacagttc
     2041 gaccattatg tcttcgaaaa cgacgacagc gtcaccgacc atattcccga aaccgcgcgc
     2101 agtatcctcg ccccgctcac gtccgagacg gcgggacggc tgcgcgcctt cttgctgagg
     2161 gccttgagta aatgagcgac acgaaatcgg ttcagcgcat cgttatcggc ggcggcggca
     2221 ccgcgggctg gatgatggcc gcggctatcg cgcgcacgat ggggcatacc gtcgatctga
     2281 cgctcgtcga gtccgaagcg atcgggacga tcggcgtagg ggaatcgacg atcccgcccc
     2341 tcgtcaattt caaccgtatc ctcggcattc ccgaaaccga gttcatgcgc gcggcgcagg
     2401 cgaccttcaa gctcggcata ttgttcgata actggaagca tgacggcgac agctatttcc
     2461 acagcttcgg cctcagcggg aaggatcact ggtcggcggg cttccagcat ttctggctca
     2521 acgcgcgcga gagggggcat gagcagccct atgccgatta ttgcctcgag ctggtcgcgg
     2581 ggatgcaggg caagttcgcg catcttcccg aagagcgcat gaactatgcc tatcatgtcg
     2641 acgcgacgct ctatggccgc tatctgcgca agctggccga agccgacggg tgcaagcgcg
     2701 tcgagggcaa gatcgcgcgc gtcgagctta acggcgagag cggcgatatc gcggcgctgc
     2761 acctcgacgg cgagcggcgg atcgagggcg atatgttcgt cgactgcacg ggctttcgcg
     2821 ggctgctgat cgacggggcg ctccacgccg ggttcgagga ctgggggcac tggctgccca
     2881 acgacagcgc gatcgcggtg caggcgaagc atcaggggcc gccgatgccc tatacgcagg
     2941 cgatcgcgca cgacgcgggc tggcaatggc gcatcccgct gcaacaccgg atgggcgcgg
     3001 ggatcgtcta ttcgagcggc tatctgtcgc gcgatgcggc ttatgaccgg ttgatgagca
     3061 gcgtcggcga accgctgatc gagccgttcg acatcaagtt ccggggcggc gtgcggcgca
     3121 agcaatggtt ccgcaactgc gtcgcggtgg ggcttgcggg cggtttcgtc gagccgctcg
     3181 aggcgacgac ggtgcatctg atccagcgcg cggtgctgcg cttcatccgg ctgatgccga
     3241 acgggcgcgt cagcgagcgc gacgcgatgg agttcaacga ccagcagcgg ctcgacatcg
     3301 agcagatccg cgacttcgtc gtcctgcact ataaagctac gaaccgccgc gacagcccct
     3361 tctggcgcca cgtcgccgcg atgccgattc ccgattcgct gcagcagaag atcgagctgt
     3421 tccgcgaaac ggggcgcgtg ttccgcaaga atgaggagct gttcgccgag aatagctggg
     3481 tgcaggtgat gatgggacag gggatcgagc cgcagagcta tcaccccatc gccgccaagc
     3541 tgcgcgacga cgaggtcgag cgcctgttcc agacgctgcg cgacaatatc gagcagacgg
     3601 tcgcgacgct gcccgagcat catgcctatg tcgcgcgcta ttgcggcgcc gaggagccga
     3661 aggcggcctg atgcgcgctg cgatatcc
//
LOCUS       DATMSU010000211         5722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_51105, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000211 DATMSU010000000
VERSION     DATMSU010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51105"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..765
                     /locus_tag="VN034_05425"
     CDS             34..765
                     /locus_tag="VN034_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60071.1"
                     /translation="MSTPAAVIIGAGDATGGAIARAFAAEGLTACVNRRTRNAEQLEA
                     LAQTIRDEGRQARAFPADARDEEAMIALFDQVEAEVGPVEVAVFNIGANVNFPITETT
                     VRVYTKVWEMACLGGFLMGREAAKRMAPRGRGTIIFTGATASLRGGSGFSAFSGAKGA
                     LRMLAQSMARELGPQGIHVAHTVIDGAIDTEFIKGRHPDFDNAKAQDLILNPAAIAAN
                     YVMLHKQPKSAWTHELDLRPWGEKW"
     gene            759..1361
                     /locus_tag="VN034_05430"
     CDS             759..1361
                     /locus_tag="VN034_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HWV60072.1"
                     /translation="MVTKTFELIFDFGSPNAYLAMKALPDLLDRTGADLVITPCLLGG
                     IFKATGNRAPMIQYADAPAKLAYENLEMRRFIAKHGLVKFRINPHFPVNTLTIMRGAI
                     VAEDEGNLDDYVDAVNRAMWEEGLKMDDPEVIAPFLSANGFDGPALLARTQEPAIKAK
                     LAANTEAAVARGVFGIPTFFVGGEMFFGKDRLAQVEEALA"
     gene            complement(1358..2278)
                     /locus_tag="VN034_05435"
     CDS             complement(1358..2278)
                     /locus_tag="VN034_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWV60073.1"
                     /translation="MTKNGGPFAGLWKQAYPLLTVTALFWAGNSIVGRAARDLVPPAA
                     LSFWRWTFALLLLLPLAWPHLKRDWPVLRANWPIVALLGALAIGSFNILLYTGLQSTT
                     ALNSMLIQSAQPALVLMAGALVMGDRTSVRQVAGVLISLAGVLAIIGRGDPAMLWGLR
                     LNIGDAIISVAVLLWALYSVLLRRRPVVHPLSFLAASMVVGIAVIVPVYAHELWSGRL
                     IVPAAGSALAIAYVSIFPSFLAYLFFNRGVELIGSAATGQYMNVMPLMGAGLAMLFLG
                     EALHLFHVAGLALIVAGILVAGRPPPAAQA"
     gene            complement(2536..3552)
                     /locus_tag="VN034_05440"
     CDS             complement(2536..3552)
                     /locus_tag="VN034_05440"
                     /inference="COORDINATES: protein motif:HMM:NF022664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3011 domain-containing protein"
                     /protein_id="HWV60074.1"
                     /translation="MRNNVVTVVTTATLIASQLAVPAMPQATTLPYPPPGGPQIQPPR
                     PGYGDGYAGTIRCESRNNKIKRCNVRTNNRVELMRVIGGRCSKGRDWGFTATQIWVSG
                     GCRAEFRYGYANNGGNPVPLPQPVDDYAGTIRCESRSNRYQQCNVATNDRVELVRRLG
                     GRCNAGRQWGYTSSYVWVNNGCRADFGYGYRNVRPPEKDKDKGPSTGLIIGGIVVAGG
                     LLALLASQSKKKKAEAGQPVQDGTATPTYPAGPPATLSANLSSLPSAARPSVQNCMND
                     AARQIGVTGGTRLSYDRLVNLEQGNGGWRIRAAMTATYPDGARTLEMYCRATPTQIIQ
                     LDFS"
     gene            3769..5055
                     /gene="murA"
                     /locus_tag="VN034_05445"
     CDS             3769..5055
                     /gene="murA"
                     /locus_tag="VN034_05445"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWV60075.1"
                     /translation="MDQIVIRGGQRLKGRIPISGAKNAALTLLPCALLTDEPLTLRNL
                     PRLADVDGFGHLLNQLGCSTTIEGSRPEDFGRVMTARTTTLTSTVAPYDIVRKMRASI
                     LVLGPLLARAGEATVSLPGGCAIGNRPIDLHLKALEAFGAEIELASGYVKASAPGGGR
                     LPGGKFTFPVVSVGATENAVMAAVLAKGTCVLENAAREPEIVDLCNCLVAMGAHIDGI
                     GTETLTIEGVDRLHGATYRVMADRIEAGSYACAAVITEGDVELVGAKADEMDATLAAL
                     REAGATVEETKTGIRVAMSGRAEPVTLSTAPYPGFATDMQAQFMAMATLGKGASLFTE
                     TIFENRYMHVPELARMGCDIQVKGRTAIVRGVDHLVGAPVMATDLRASMSLIIAGLAA
                     EGQTEVNRVYHLDRGYERLEEKLQAVGADIERISAG"
     gene            complement(5042..>5722)
                     /locus_tag="VN034_05450"
     CDS             complement(5042..>5722)
                     /locus_tag="VN034_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV60076.1"
                     /translation="GSQGGWVAPLAATRTKADFVAVGFGLVASPIEEDREQMVSEVRA
                     AGLGKDAEALVDRLSAATSKLVLSNFTQGYDALDAVRREMAGQSWASAIKGEYSGAIA
                     RMTNDELRRLGRARFDNVELIWDYAAVAALRKLDTPLLWVLAGEDREAPIETTRGALL
                     GLAKGGKPFDVYLFPDTDHGMFEFTTAADGSRSITRITDGYLKLLGDWIKGDTHGAYG
                     RAQRLTPR"
BASE COUNT         1024 a         1967 c         1816 g          915 t
ORIGIN      
        1 taattactat tggacgcaag aggagagtcg ccgatgtcca cccccgcagc cgtcatcatt
       61 ggagccggcg acgccaccgg cggcgctatc gcccgcgcct tcgccgccga aggcctcacg
      121 gcctgcgtca accgccggac acgaaacgcc gagcaactcg aagcgctcgc gcagacgatc
      181 cgcgacgaag ggcgtcaggc gcgcgccttc cccgccgatg cgcgcgacga ggaagcgatg
      241 atcgcgctgt tcgaccaggt cgaggccgaa gtcgggccgg tcgaggtcgc ggtgttcaac
      301 atcggcgcca atgtgaattt cccgatcacc gagacgacgg tgcgcgtcta taccaaggtc
      361 tgggaaatgg cgtgcctcgg cggtttcctg atgggccgcg aggcggcgaa gcgcatggcg
      421 ccgcgcggcc gcggtacgat catcttcact ggcgcgactg cgtccttacg cggcggatcg
      481 ggcttttcgg ccttctcggg cgcgaagggc gcgctccgca tgctcgccca gtcgatggcg
      541 cgcgaactcg ggccgcaggg cattcatgtc gcgcacacgg tcatcgacgg cgcgatcgac
      601 accgaattca tcaagggccg ccatcccgat tttgacaatg cgaaagcaca ggatctgatc
      661 ctcaaccccg cagccatcgc cgccaattat gtgatgctcc acaagcagcc caaaagcgcg
      721 tggacgcacg aactcgacct tcgcccgtgg ggagaaaaat ggtgaccaaa acgttcgaac
      781 tcatcttcga cttcggcagc cccaacgcct atctcgcgat gaaggcgctc cccgatctgc
      841 tcgaccgcac cggcgccgat cttgtcatca ccccctgtct tttgggcggc atcttcaagg
      901 cgacgggcaa cagggcgccg atgatccaat atgccgacgc gccggcgaag ctcgcttatg
      961 agaatctcga aatgcgccgg ttcatcgcga agcacggcct cgtcaaattc cgcatcaatc
     1021 cgcacttccc ggtcaacacg ctgacgatca tgcgcggcgc gatcgtcgcc gaggacgaag
     1081 gcaatctcga cgattatgtc gacgcggtga accgcgcgat gtgggaggaa ggcctcaaga
     1141 tggacgatcc cgaggtgatc gcacccttcc tctcggcgaa cggcttcgac ggcccggcgc
     1201 tgctcgcgcg cacccaggag cccgcgatca aggcaaagct ggccgccaac accgaagccg
     1261 cggtcgcgcg cggcgtgttc ggcatcccga ccttcttcgt cggcggcgag atgttcttcg
     1321 gcaaggaccg gctggcgcag gtcgaggaag ctctcgccta ggcttgcgcc gcaggcggcg
     1381 ggcgcccggc gacgaggatc ccggcgacga tcagcgccag cccggcaaca tggaacaggt
     1441 gcaacgcctc gccgaggaac agcatcgcca gccccgcgcc catcagcggc atcacgttca
     1501 tatattgccc cgtcgccgcc gatccgatca gttcgacccc gcgattgaag aagagatagg
     1561 cgaggaagga cgggaagatc gagacatagg cgatcgcgag cgcgctcccc gccgccggaa
     1621 cgatcagccg ccccgaccag agttcgtgcg catagacggg cacgataacc gcgatgccga
     1681 cgaccatcga ggctgcaagg aaactcagcg gatgcaccac agggcggcgg cgaagcagca
     1741 ccgaataaag cgcccagagc aggaccgcca cgctgatgat cgcgtccccg atattgagcc
     1801 ggagacccca cagcatcgca ggatcgccgc gcccgatgat cgcgagcacg ccggcgagcg
     1861 agatcagcac ccccgcaacc tgccgcacgc tcgtgcgatc gcccatcacc agcgctccgg
     1921 ccatcagcac aagcgcgggc tgggctgact ggatcagcat cgaattgagc gcggtggtgc
     1981 tctgcagccc cgtatagagc aggatgttga atgagccgat cgccagcgcg ccgaggagcg
     2041 cgacgatcgg ccagttcgcg cgcagcaccg gccaatcgcg cttcaggtgc ggccatgcga
     2101 gcggcagcag cagcaagagc gcgaaggtcc agcgccagaa ggacaaggcg gccggcggca
     2161 ccaggtcgcg cgcggcgcgc ccgacgatcg aattgcccgc ccagaacagc gcggttaccg
     2221 tcagcagcgg ataagcctgt ttccaaagcc ccgcgaacgg gccaccgttt ttcgtcattc
     2281 cctcttggcc ccgtccattc cgtcagatgc tccttccttt cacggcttga gcgaaagatg
     2341 caatccgggc gggcgcggcg ggtttcggcc ggtagcgaca ttatctcccc tcccgcaagc
     2401 gggaggggct gggagtgggc agcaacggtg caatggccca ccccgctgcg actaacgaac
     2461 aaagttcgta agtctcgctg cccctcccgc ttgcgggagg ggaaatcagc tcacgacctc
     2521 gacggcagtt caaccctaac tgaagtcgag ctggatgatc tgcgtcggcg tcgcgcggca
     2581 gtacatttcg agcgtgcgcg cgccgtcggg ataggtcgcg gtcattgccg cgcggatgcg
     2641 ccagccgccg ttgccctgct cgaggttgac cagccggtcg tacgacagcc gcgtgccgcc
     2701 ggtcaccccg atctgccgcg ccgcatcgtt catgcaattc tgcaccgacg gtcgcgccgc
     2761 gctcggcagg gacgagaggt tggcgctcag cgtcgccgga ggccccgccg gataggtcgg
     2821 cgttgccgtg ccgtcctgga ccggctgacc ggcttcggcc ttcttcttct tgctctggct
     2881 cgcgagcagg gcaagcagcc ctcccgcgac gacgatgccg ccgatgatca gcccggtgct
     2941 cggccccttg tccttgtcct tttcgggtgg ccggacgttg cggtagccat agccgaagtc
     3001 ggcgcggcat ccgttgttca cccagacata gctcgaggta tatccccatt gccgcccggc
     3061 gttgcagcgc ccaccgagcc ggcgcaccag ttcgacgcgg tcgttcgtcg cgacattgca
     3121 ctgctgatag cggttgctgc gcgattcgca gcggatcgtc cccgcataat cgtcgaccgg
     3181 ctgcggcaac ggcacgggat tgccgccatt attggcatag ccatagcgga actcggcgcg
     3241 gcatccgccc gacacccaga tctgcgtcgc cgtgaaaccc cagtcgcgcc ctttcgaaca
     3301 gcgcccgccg atgacgcgca tcaattcgac gcgattgttc gtgcggacat tgcagcgctt
     3361 gatcttgttg tttcgcgatt cgcagcggat cgttccggca tagccgtcgc catagccggg
     3421 acgcggcggc tggatctgcg ggccgccggg tggcggatag ggcaaggtcg tggcctgcgg
     3481 catcgcgggc acggcaagtt ggctggcaat caaagtggcc gtagtcacga ccgtgacgac
     3541 attattccgc atgaccttct cccctgtgaa aagtcgtttt acgtaaacgc tgcgattgtc
     3601 gaggcccaat atcggcacga atagtttcac attttcgatg ggaaagccac gatgaaagct
     3661 attcgctcat acggcaactt aaccatcatc gcggcgccgt ccggcggccc ccatcgccgc
     3721 catggccagt ccgcttgcgc ggccccgcgc atgcgctaaa gacagccgat ggatcagatc
     3781 gtcattcgcg gcggccagcg actcaagggc cgtatcccca tctccggtgc caagaatgcg
     3841 gcgctcaccc tgttgccctg tgcgctgctc accgacgagc cgctgacgct caggaacctg
     3901 ccgcgactcg ccgatgtcga cgggttcggc cacctcttga accagctcgg ctgctcgacg
     3961 acgatcgagg gatcgcggcc cgaggatttc ggccgcgtga tgaccgcgcg cacgacgacg
     4021 ctgacctcga ccgtcgcgcc ctatgacatc gtgcgcaaga tgcgcgcgtc gatcctcgtg
     4081 ctcggaccgc tgctcgcgcg cgcgggcgag gcgaccgtgt cgctgcccgg cggctgcgcg
     4141 atcggcaacc gcccgatcga cctccatctg aaagccctcg aagccttcgg cgccgagatc
     4201 gaactcgcgt cgggctatgt gaaggcaagc gcgcccggcg gcggacgcct tcccggcggc
     4261 aagttcacct tccccgtcgt ctcggtcggc gcgaccgaaa atgccgtcat ggcggcggtg
     4321 ctcgccaagg gcacatgcgt gctcgaaaat gcggcgcgcg agcccgagat cgtcgacctc
     4381 tgcaattgcc tcgtcgcgat gggcgcgcat atcgacggca tcggcaccga aacgctgacg
     4441 atcgagggcg tcgaccgcct gcacggcgcg acctatcgcg tcatggccga ccgcatcgaa
     4501 gcgggcagct acgcctgcgc cgcggtcatc accgagggcg atgtcgaact cgtcggcgcc
     4561 aaggccgacg agatggacgc gacgctcgcg gcgctgcgcg aagccggcgc gacggtcgag
     4621 gagaccaaga ccggaatccg cgtcgccatg tcgggccgcg ccgaacccgt caccctgtcg
     4681 accgcgccct atccgggctt cgcgaccgac atgcaggcgc agttcatggc gatggcgacg
     4741 ctgggcaagg gcgcgtcgct gttcaccgaa acgatcttcg aaaaccgcta tatgcacgtc
     4801 cccgaacttg cgcgcatggg ctgcgacatt caggtcaagg gccgcaccgc gatcgtccgc
     4861 ggggtcgatc acctcgtcgg cgcgccagtg atggcgaccg acctgcgcgc ctcgatgagc
     4921 ctgatcatcg cgggactcgc cgccgagggg caaaccgagg tcaaccgcgt ctatcacctc
     4981 gaccgcggtt acgagcggct cgaggagaag ctccaggccg tcggcgccga catcgagcgg
     5041 atcagcgcgg ggtgagccgc tgtgcgcggc cataggcgcc gtgcgtatcg cccttgatcc
     5101 aatcgccgag caatttgaga tagccgtcgg tgatgcgcgt tatgctgcgc gatccgtcgg
     5161 cggcggtcgt gaactcgaac atgccgtggt cggtatcggg gaagaggtag acgtcgaacg
     5221 gcttgccccc cttcgcgagg cccagcagcg cgccgcgggt cgtttcgatc ggcgcctcgc
     5281 ggtcctcacc cgcgagcacc cacagcaagg gcgtatcaag cttccggagc gcggcgaccg
     5341 cggcataatc ccagatcagc tcgacattgt cgaagcgcgc acggccgagg cgcctcagtt
     5401 cgtcgttcgt catgcgcgcg atcgcgccgc tatactcgcc tttgatcgcc gacgcccatg
     5461 actgccccgc catttcgcgc cgcaccgcat cgagcgcgtc atagccttgc gtgaagttcg
     5521 acagcaccag tttcgacgtc gccgccgaca gccgatccac gagcgcctcg gcatccttgc
     5581 ccagccccgc cgcgcgcact tcggacacca tctgctcgcg gtcttcctcg atcggtgagg
     5641 cgacgaggcc gaagccgacc gcgacaaaat cggcctttgt ccgcgtcgca gcgagcgggg
     5701 cgacccagcc gccctggctg cc
//
LOCUS       DATMSU010000212         7931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_108142,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000212 DATMSU010000000
VERSION     DATMSU010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108142"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VN034_05455"
     CDS             complement(<1..228)
                     /locus_tag="VN034_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWV60077.1"
                     /translation="MRLAAPRIEPVDLDRLDADQRAALEPFLASDGGKVGGGKILNIF
                     RTLAQAPKALTAFLGWGNYILSKRNALSPRDR"
     gene            complement(228..1409)
                     /locus_tag="VN034_05460"
     CDS             complement(228..1409)
                     /locus_tag="VN034_05460"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWV60078.1"
                     /translation="MRDAVIVSTARTPLTKAARGSFNNTLAPTLGAFSVKAAVERAGL
                     EGGEIDDVLFGAAMQQGSQTMNIGRLVALRAGLPVTVPGMSIDRQCSSGLMTIATAAK
                     QIIVDRQDIAVAGGIESISKVSGSGKVFIETDAELIAMHKDTYMPMIGTAEVVAKRYN
                     ISREAQDEYSLQSQQRTAAAQAAGKYDDEIIACNATMAVMNKETKEVSFKDVVADKDE
                     CNRPDTTLEGLSSLKPVMGEGHTITAGNASQLADGSSACVVMEAKVAEKRGLQPLGRY
                     VGMAVAGTEPDEMGIGPVFAIPKLLERFELKMDDIGLWELNEAFAVQVLYCRDKLGIP
                     NELLNVNGGSISIGHPFGMTGARCVGHALIEGKRRGVKYAVVTMCIGGGQGAAGLFEV
                     F"
     gene            complement(1412..1912)
                     /locus_tag="VN034_05465"
     CDS             complement(1412..1912)
                     /locus_tag="VN034_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWV60079.1"
                     /translation="MKILMRGADPLVLFTTLATNERAWSKFAGGALLDKASPLSLRQR
                     ELVIDRTTAKCGCEYEWGVHVAGFADRIGLDADQVHAIVHGDPDAPVWSEDERALLSA
                     VDTLLATKKLSDEQWKALRAHFDDDQTMEIVQLVAFYHGVSLICGALDLPLESNAARF
                     PAAIGD"
     gene            complement(1976..2434)
                     /locus_tag="VN034_05470"
     CDS             complement(1976..2434)
                     /locus_tag="VN034_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWV60080.1"
                     /translation="MATITPQDLQAKVGEHLGTSEWVLVDQDMINKFADATGDHQFIH
                     IDEEKAKLTPFGGTIAHGFLTLSLIPMLGARTDGPKIEGVKMGVNYGGNKVRFLAPVR
                     SGKRVRSHVKLLELEEKRPGQWQQTNEVTIEIEGEEKPALIAEWISQFFV"
     gene            complement(2530..3726)
                     /locus_tag="VN034_05475"
     CDS             complement(2530..3726)
                     /locus_tag="VN034_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWV60081.1"
                     /translation="MTFSLFAAATTAAPKAAPARTPTTNPVEDLQRYWDMSVAWVNGH
                     WLQIGIAIGAGLAIYFILSMLRRFALKYAQSAEGEMSLTHIAGRVIHKTKSAVLAIIA
                     IRLVAGYAQPPTVIMQIVQLVFTVAVVLQVAIWVREIVLGLIQRRAAEGHNETLSNAM
                     GIIRLLISVALFAIAAIVILDNMGVNVTGLVAGLGIGGIAIGLAAQGIFSDLFASLSI
                     IFDRPFRVGETIKYDMSTATVERIGLKSTRLRSVNGELLVISNTNLLAKEITNFAHLH
                     RRRVTFMIGVIYQTTPAMLRDLPALLEEQVKAAGQEFIRSSFVTFGPSSLDFELLFDV
                     FSDDYDVVTAARTDVAIRLFEAMAGAGYEFAYPTQTTFTAAPDGTMILPYAESVTMKT
                     PKPAKS"
     gene            complement(3759..4769)
                     /locus_tag="VN034_05480"
     CDS             complement(3759..4769)
                     /locus_tag="VN034_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWV60082.1"
                     /translation="MAATRLVHVDDSLPGISRARSGVGWRYRDAKGKTIRDRDEIDRL
                     NAIALPPAYEDAWFCPAPNGHILATGYDARGRKQYRYHPDFRMAREADKYDRCAAFGH
                     ALPLLRARLADDLARRTLCSERVVAAVVRLLDLAALRIGNEQYVTANKSFGATTLRQR
                     HAKASGKTLRLQYVAKGGAKRDVTISDRSLSTLVRRLQDLPGQKLFQYEDADSLCAVG
                     SDDVNAYIREAMGDEFSAKHFRTWSASVEAFAFLYDAPEPPTLKAMLEHVADRLGNTP
                     AIARKAYVHPAMIAAAQERADFAAIVGTLPRATRYLSRYERGFLTYLEQAPAAAVLLQ
                     SA"
     gene            complement(4808..6190)
                     /locus_tag="VN034_05485"
     CDS             complement(4808..6190)
                     /locus_tag="VN034_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HWV60083.1"
                     /translation="MRDIAFVAFLFAFIGLGFRKPFLFVLCFCYIDIVAPQRLSYFLI
                     NSIPISLIVFGLAIVGWLAVDDKKDVRWSGRQLLLIVLLLYCWMTTIQADFPVEAADK
                     WSWVWKALVWAIFLPLTLRTKLRIESLVLVMLLSAAAIAIAGGIKTAAGGGGYGSLQL
                     LLNENYGLYEGSIMSAVGISIIPLILWYREHGTIFPPDWRVSIFCFALVFACALLPIG
                     TQARTGLVCLVILAILSLRAVKHRFLYMAGAGLLALAAIPFLPQSFTERMETIRDHKS
                     DQSASTRVAVWAWTWEYAKENPLGGGFNAYIQNHVRVERAASDYDPDAPQSNEPTVYE
                     EHSRAYHSSYFEMLGEQGYPGLALWLLLHAVGLLRMEQLRRRYLKTRRGEEQWIAPLA
                     TALQHGHIVYMIGSLFVGIAFQPFIYMMLALEMGLSTYVRRREQEAGWRPLTARPAQV
                     PARHPVATPG"
     gene            complement(6183..6347)
                     /locus_tag="VN034_05490"
     CDS             complement(6183..6347)
                     /locus_tag="VN034_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60084.1"
                     /translation="MVDNFSIGLTHLLMAIALWRLLYRDDLDREVSPRMLWQQRRDAE
                     RAEADSRADA"
     gene            6413..7618
                     /locus_tag="VN034_05495"
     CDS             6413..7618
                     /locus_tag="VN034_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04063 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HWV60085.1"
                     /translation="MTRILHVLDHSLPAHSGYTFRTRALMKAQVAKGWAVAGVTGVRH
                     PEPGADGETVDGLTFYRTLPIAPARAPIREWREIGALAKRVEALAKEWKPDVLHAHSP
                     VLDGLAALRVGKKLGIPVIYEIRAFWEDAAVGNGTGREGSLRYRLTKRLETHAVKAAD
                     AVAVICEGLRCDLIARGIDPAKIIVSPNGVDLDLFGDPPPRDDALAETLGLAADDVVI
                     GYIGSFYDYEGIDDLIAAMPALAAAQPKARLLLVGGGPMETALKAQAASSPVAAQILF
                     VGRVPHGEVERYYSLIDILAYPRKKMRLTDLVTPLKPLEAMAQGKLVAASNVGGHREL
                     IEDGVTGTLFAPDDPAAIANALAGLLDNRAMWPERRRTARIFVESHRNWSSNILRYEP
                     VYQRLLRGA"
     gene            7678..>7931
                     /locus_tag="VN034_05500"
     CDS             7678..>7931
                     /locus_tag="VN034_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60086.1"
                     /translation="MDETSENRAKAIATALLRALQPAIPAVIGAAALTFLFAALMPFS
                     WVAAISWNLYLDRLSDLFVPPIGNGGRLALALGMSAVAAA"
BASE COUNT         1384 a         2485 c         2647 g         1415 t
ORIGIN      
        1 gcggtcgcgt gggctgagcg cattgcgctt cgaaaggata taattgcccc agccgagaaa
       61 ggccgtgagc gccttgggcg cttgggcaag ggtgcggaag atgttgagga tcttgccgcc
      121 cccgaccttg cccccatcgg aggcaaggaa gggttcgagc gcggcgcgct ggtcggcatc
      181 gagccggtcg aggtcgaccg gctcgatacg cggagcggca agccgcatca gaagacctca
      241 aagaggcccg ctgcgccctg accgccgccg atgcacatgg tgacgacggc atatttcaca
      301 ccgcggcgct tgccctcaat gagtgcgtgg ccgacgcagc gtgcgcccgt catcccgaag
      361 gggtggccga tcgagatcga gccgccgttg acgttgagca gctcgttcgg aatgccgagc
      421 ttgtcgcggc agtaaagcac ctgcaccgca aaggcttcgt tgagttccca gaggccgatg
      481 tcgtccatct tgagctcgaa gcgttcgagc agcttcggga tcgcgaagac cgggccgatg
      541 cccatttcgt cgggctcggt gcccgcgacc gccatgccga catagcggcc gagcggctgg
      601 agcccgcgct tttcggcgac cttggcttcc atcacgacgc aggccgacga tccgtcggcg
      661 agctggctcg cgttgcccgc ggtgatcgtg tggccttcgc ccatcaccgg cttcaggctc
      721 gaaagccctt cgagcgtcgt gtcgggacgg ttgcactcgt ccttgtcggc gacgacgtcc
      781 ttgaagctga cttccttggt ttccttgttc atcaccgcca tggtcgcgtt gcatgcgatg
      841 atctcgtcgt catatttgcc tgcggcctgg gcggcggcag tgcgctgctg cgactggagc
      901 gaatattcgt cctgcgcttc gcggctgatg ttgtagcgct tcgcgacgac ctcggcggtg
      961 ccgatcatcg gcatataggt gtccttgtgc atcgcgatca gctcggcgtc ggtttcgatg
     1021 aacaccttgc cgctgccgct gaccttcgag atcgactcga tgccgccggc aaccgcgatg
     1081 tcctgacggt cgacgatgat ctgtttcgca gcggtcgcga tcgtcatcag gcccgacgag
     1141 cattggcggt cgatcgacat gccggggacg gtgacgggca ggccggcgcg cagcgcgacg
     1201 aggcggccga tgttcatcgt ctgcgatccc tgctgcatcg cggcgccgaa gaggacgtcg
     1261 tcgatctcgc cgccttcgag gccggcgcgt tcgactgcgg ccttgaccga gaaggcgccg
     1321 agcgtcgggg cgagggtgtt gttgaacgag ccgcgcgccg ccttggtcag cggggtgcgg
     1381 gcggtggaaa cgatgacggc gtcacgcatg attaatctcc tatggcggcc gggaatcgcg
     1441 ccgcattgct ttccaagggg aggtcgagcg cgccgcagat cagcgacacg ccgtgataaa
     1501 aggcaacgag ctggacgatc tccatcgtct ggtcgtcgtc gaaatgcgcg cggagcgcct
     1561 tccattgctc gtcggacaat ttcttggtgg cgagcaacgt atcgacggcg gacaataatg
     1621 cgcgctcgtc ctcgctccac accggcgcgt cgggatcgcc atgcacgatc gcgtgcacct
     1681 gatcggcatc gaggccgatt cgatcggcaa agccggcgac gtggacgccc cattcatatt
     1741 cgcagccgca ttttgcggtg gttcggtcga tcaccagttc gcgctggcgc agcgacaagg
     1801 ggctcgcctt gtcgagcagc gcgccgccgg caaacttgct ccacgcccgt tcgttggtcg
     1861 ccagtgtcgt gaacaggacc agcggatcgg cgccgcgcat caagatcttc atgcgcgcat
     1921 cgaattccgg cggatacggc ttttcaagag gagtgatgcg cgccatcgga ctctatcaga
     1981 cgaagaactg gctgatccat tcggcgatca gcgcgggctt ttcctcgccc tcgatctcga
     2041 tcgtcacttc attggtctgt tgccactggc cggggcgctt ctcttcgagt tcgagcagct
     2101 tcacatggct ccgaacgcgc ttgcccgagc ggacgggggc gagaaagcgg accttgttgc
     2161 cgccatagtt gacgcccatt ttgacgcctt cgatcttcgg cccgtcggtg cgcgcgccga
     2221 gcatcgggat cagcgacagc gtcaggaagc cgtgcgcgat ggtgccgccg aagggcgtga
     2281 gcttcgcctt ttcctcgtcg atgtggatga actgatggtc gcccgttgca tcggcgaact
     2341 tgttgatcat gtcctgatcg acgagcaccc attcggacgt gcccagatgc tcaccgacct
     2401 tggcttgcag gtcctgcggc gtgatggtgg ccatatctca tcccctcgaa ttgctggttc
     2461 ggggcgcgtt ctaggcctct cttgccgcat tgcacaaggg ccaagccccg cggccgcgtc
     2521 gccgtagcgt cagctcttgg cgggcttcgg cgtcttcatc gtcacgctct cggcataggg
     2581 tagaatcatc gtgccgtcgg gcgccgcggt gaaggtggtc tgggtcggat aggcgaattc
     2641 gtagcccgcg cccgccatcg cttcgaacag ccggatcgcg acgtcggtgc gcgcggcggt
     2701 gaccacgtca taatcgtcgc tgaacacgtc gaagagcagt tcgaagtcga gactcgacgg
     2761 gccgaaggtg acgaagctcg aacgaatgaa ctcctgcccg gccgccttga cctgttcctc
     2821 gagcagtgcg ggcaggtcgc gcagcatcgc gggcgtcgtc tggtagatca cgccgatcat
     2881 gaaggtcacg cggcggcgat ggagatgcgc gaaattggtg atctccttcg cgagcagatt
     2941 ggtgttcgaa atgacgagca gctcgccgtt gacggagcgc agccgcgtgc tcttgaggcc
     3001 gattcgctcg accgtagcgg tgctcatgtc atatttgatc gtctcgccga cgcggaacgg
     3061 ccggtcgaag atgatcgaga gcgaagcgaa gaggtcggag aagatcccct gcgcggcaag
     3121 gccgatcgcg atgccgccga tgccgaggcc ggcgaccagc cccgtgacgt tgacgcccat
     3181 attgtcgagg atcacgatcg cggcgatcgc gaataaggcg acgctgatca gcaggcggat
     3241 gatacccatc gcgttcgaca gcgtttcgtt gtgcccttcg gcggcccggc gctggatcag
     3301 cccgagcacg atctcgcgca cccagatcgc gacctgcagc accaccgcga ccgtaaagac
     3361 gagctggacg atctgcatga tcaccgtggg gggctgcgca tagcccgcga ccaggcggat
     3421 cgcgatgatc gcgagcacgg ccgacttggt cttgtggatc acccgcccgg cgatgtgggt
     3481 cagcgacatc tcgccttcgg ccgactgcgc atatttgagt gcgaagcggc gaagcatcga
     3541 gaggatgaaa tagatggcga ggccggcacc gatcgcgatg ccgatctgca gccagtggcc
     3601 gttcacccac gcgacgctca tatcccaata gcgttgcagg tcttcgacag gattggtggt
     3661 cggggttcgt gcgggcgcgg ctttcggtgc ggcggtggtc gcggcggcga acagggagaa
     3721 ggtcatgcag gggtccttgg gggtcctcta gaatcggatc aggcggattg gagcagcacc
     3781 gcggccgcgg gcgcctgctc cagataggtc aaaaatccgc gttcgtaacg cgaaagatag
     3841 cgcgtcgcgc gcggcagcgt gcccacgatc gccgcgaagt ccgcgcgctc ctgtgccgcc
     3901 gcgatcatcg ccgggtggac ataggccttg cgcgcgatgg cgggggtgtt gccgaggcgg
     3961 tcggcgacat gctcgagcat agccttcagc gtcggcggct cgggcgcgtc gtaaaggaag
     4021 gcgaaggcct cgacgctggc cgaccatgtg cggaaatgct tggcgctgaa ttcatcgccc
     4081 atcgcctcgc gaatataggc attcacatcg tccgatccga ccgcgcagag gctgtccgca
     4141 tcctcatatt ggaaaagctt ctgccccggc agatcctgca accggcgcac cagcgtcgaa
     4201 aggctgcggt cgctgatcgt cacgtcgcgt ttcgcgccgc ccttggcgac atattgcagg
     4261 cgcaacgtct tgcccgacgc tttcgcatgg cgctggcgca gcgtcgtcgc cccgaagctc
     4321 ttgttcgctg tgacatattg ctcgttgccg atgcgcagcg cggcgaggtc gagcaggcgc
     4381 acgaccgccg cgacaacgcg ttccgagcac aaagtccggc gggcgagatc gtcggcgagg
     4441 cgcgcgcgga gcaatggcaa cgcatggccg aaggcggcgc agcggtcata tttgtcggcc
     4501 tcgcgcgcca tccggaagtc gggatgatag cgatattgct tgcgtccgcg cgcgtcatag
     4561 ccggtcgcga ggatatggcc gttcggcgcc ggacagaacc aggcgtcttc gtaagccggg
     4621 ggcagcgcga tcgcgttgag gcggtcgatt tcgtcgcggt cgcggatcgt cttgcccttg
     4681 gcatcgcgat agcgccagcc gacccccgaa cgtgcgcggc tgatcccggg caggctgtcg
     4741 tcgacatgga caaggcgggt tgcggccatg cgtccatagc gccttgctgc gccatatggt
     4801 tccggggtca gccgggcgtg gccaccggat gccgcgccgg aacctgtgcc gggcgcgcgg
     4861 tcagcgggcg ccatcccgct tcctgttcgc ggcggcgcac ataggtcgac agtcccattt
     4921 cgagcgcgag catcatgtag atgaaaggct ggaaggcgat gccgacgaac agcgacccga
     4981 tcatatagac gatatggccg tgctggagcg cggtcgcgag cggcgcgatc cattgctcct
     5041 cgccgcgcct tgtcttcaga tagcgccggc ggagctgctc cattcgcagc agcccgactg
     5101 cgtgcaggag cagccataac gccagccccg gataaccctg ttcgccgagc atctcgaaat
     5161 agctcgaatg ataggcgcgc gaatgttcct cataaaccgt cggttcgttg ctttgcggcg
     5221 cgtcggggtc atagtcgctc gccgctcttt cgacgcggac atggttctgg atataggcat
     5281 tgaagccgcc gcccaacggg ttttccttcg catattccca cgtccacgcc cacaccgcga
     5341 cgcgcgtcga cgccgactgg tcggatttat ggtcgcggat cgtttccatc cgctcggtaa
     5401 agctttgcgg caggaagggg atggcggcga gcgcgagcaa ccccgcgccc gccatataga
     5461 gaaagcggtg cttcaccgcg cgcagcgaca ggatcgcgag gatgacgagg cagacgagcc
     5521 cggtgcgtgc ctgcgtgccg atcgggagca gcgcgcaggc gaagacgagc gcgaagcaga
     5581 agatcgagac gcgccagtcg ggcgggaaga tcgtgccatg ctcgcgatac cagaggatca
     5641 gggggatgat cgaaatgccg accgccgaca tgatcgagcc ttcgtacagg ccgtaattct
     5701 cgttgagcag caactgcagc gagccatagc cgccgccgcc cgctgcggtc ttgatcccgc
     5761 ccgcgatcgc gatcgccgcc gctgacagca gcatgacgag aaccagcgat tcgatacgca
     5821 gcttggtccg gagcgtgagc ggcaggaaga tcgcccagac gagcgccttc cacacccagc
     5881 tccatttatc ggccgcctcg accgggaagt cggcctggat cgtcgtcatc cagcaataga
     5941 gcagcagcac gatcagcagc aattgccgcc ccgaccatcg cacatccttc ttgtcgtcga
     6001 ccgcgagcca gccgacgatc gccagcccga agacgatcag cgagatcggg atcgagttga
     6061 tgaggaaata gctgaggcgt tgcggcgcga cgatgtcgat atagcaaaag cacagcacga
     6121 acaggaacgg cttgcgaaag ccgagcccga tgaaggcgaa gagaaaggcg acaaaggcga
     6181 tgtcacgcat cggcgcggga atccgcttcg gcacgctcgg cgtcgcggcg ctgctgccag
     6241 agcatgcgcg ggctcacttc gcggtcgaga tcgtcgcgat agagcaggcg ccagagcgcg
     6301 atcgccatca gcaaatgcgt aaggcctatg gagaaattgt cgaccatgtc cgctcccgct
     6361 acgcgatcgg ggttgacgcc gcgttaaggc ttgtatgtca gtggactggc caatgacccg
     6421 tatactacac gtccttgatc atagcctccc ggcgcacagc ggctacacct ttcgcacgcg
     6481 cgcgttgatg aaggcacagg tggcgaaggg ctgggcagtc gcgggcgtca ccggcgtgcg
     6541 ccaccccgag ccgggcgccg acggcgagac ggtcgacggg ctgacctttt accgtaccct
     6601 gccgatcgca ccggcacgcg cgccgatccg cgaatggcgc gaaatcggcg cgctggcaaa
     6661 gcgggtcgag gcgctggcga aagaatggaa gcccgacgtg cttcacgcgc attcgccggt
     6721 actcgacggg cttgcggcgc tgcgcgtcgg caagaagctg ggcatcccgg tcatctatga
     6781 aatccgcgcc ttttgggagg atgcggcggt cggcaacggc accggacgcg aaggcagcct
     6841 gcgttaccgg ctgaccaaaa gactcgaaac ccatgcggtg aaagccgccg atgccgtcgc
     6901 ggtgatctgc gaaggcctgc gctgcgacct gatcgcgcgc ggcatcgatc ccgccaagat
     6961 catcgtgtcg ccgaacggcg tcgacctcga cctcttcggc gacccgccgc cgcgcgacga
     7021 tgcgctcgcc gaaacgctgg gcctggccgc ggacgacgtc gtgatcggct atatcggcag
     7081 cttttacgac tatgagggga tcgacgatct gatcgcggcg atgcccgcgc tggccgccgc
     7141 gcagccgaag gcgcggttgc tgctggtcgg cggcggcccg atggagaccg cgctcaaggc
     7201 gcaggcggcg tcgtcgcccg tcgcggcgca aatcctcttc gtcggccgcg tgccccacgg
     7261 cgaggtcgaa cgctattatt cgctgatcga tattttggcc tatccacgca agaagatgcg
     7321 gctcaccgac ctcgtcacgc cgctgaaacc gctcgaggcg atggcgcagg gcaagctggt
     7381 cgcggcgtcc aacgtgggcg gccaccgcga gttgatcgag gacggcgtga cgggaacgct
     7441 gttcgcgccc gatgacccgg ccgcgatcgc caacgcgctc gccggcttgc tcgataatcg
     7501 cgcgatgtgg cccgaacgcc ggcgaaccgc acgtattttt gtcgaaagcc atcgtaactg
     7561 gtcgtcaaac attttacgtt acgagccggt ttaccagcga ttgctgcgcg gcgcctgagg
     7621 gcgacgcgct tgatgtgagg acaggacggc ccggcgggcc gacggatttg ggacgatatg
     7681 gacgaaacca gcgagaatcg ggccaaggcc atagcgaccg cgctgctgcg cgcgctccag
     7741 cctgcgatcc cggcggtgat cggcgccgcg gcgctgacct tcctgttcgc cgcgctgatg
     7801 cccttttcat gggtcgcggc gattagctgg aatctttatc tcgaccgcct ctccgacctg
     7861 ttcgtcccgc cgatcggcaa tggcggacgg ctcgcgcttg cgctcggcat gtcggcggtc
     7921 gccgccgcga t
//
LOCUS       DATMSU010000213         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_22450, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000213 DATMSU010000000
VERSION     DATMSU010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22450"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2240)
                     /locus_tag="VN034_05505"
     CDS             complement(<1..2240)
                     /locus_tag="VN034_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWV60087.1"
                     /translation="MRSETGFDPALYDRFPTKGPRPEGEEEALRRIWCKPKGWEYLTV
                     VNNNYVGIYYLGTAFLFFLLAGVLALLMRAQLAAPMLEILPQGTYNQVFTMHGTVMMF
                     LFAVPAVEAAGVLLLPQMLAARDLPFPRLSAYAFWAYAVGGLCFFASIFVGLAPDGGW
                     FMYPPLTSKAYSPGINTDFWLLGIGFIEISAIAGAVEIIVGVLRTRAPGMSLDKMPIF
                     AWAMLVFAVMIVIAFPSIILGTLLLEIERAFNWPFFDATRGGDPLLWQHLFWFFGHPE
                     VYIIFLPAAGMMSMIVAAVAREQLVGYRLIVLAMVATGFISFGVWAHHMFTTGMPPMT
                     TGFFSAASMAVSVPAGIQVFSWIATLAVGTPRFNAPGLFILGSIVIFVTGGLTGVMVA
                     MVPFDWQAHDSYFIVAHLHYVLIGGMVFPLFAAFYYWIPMVSRRALSERLAKWVFGLM
                     FAGMNIAFLPMHLAGLQGMPRRVYTYLPGQGLELPNLISTIGAFMLGAGVLLFLVDLA
                     RNFRLAPGEGNAGNVYGGGTLEWLPSELYSTRSIPVVTSREPLWDDPALADDVAAGRY
                     FLPGSATGLRETIVTSPLLAEPQYLQIMPGPSPWPFAAAIFTAGFFLALTVQAYAFAW
                     ASGVLAIIAVLRWLWDTDRRVEAERVDIGAGIEVPTYVTGPSSHGWWAMVILLVVMGM
                     IFVMALFSLAFLWSNQPAFWMTPPPLGEAAAVLLLYGVAFLLALAGRGALRWRPSASV
                     ALLLASA"
     gene            complement(2237..3178)
                     /gene="coxB"
                     /locus_tag="VN034_05510"
     CDS             complement(2237..3178)
                     /gene="coxB"
                     /locus_tag="VN034_05510"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWV60088.1"
                     /translation="MTDRLPETGWPPPVLDPAGPYSEPVTTLAWALLIGGTVVFAIVL
                     AALWIALRGSPKLKTQLGGEKAIWILGFAFPSVVLTGLLVWGLMLTSSLSATRIPEGA
                     MRVKIIGEMWWWRVHYQDGRGRETLRDANELHIPVGTPVLLELEAADVIHSFWVPRLG
                     GKMDMIPGRTNRLLIEADAPGVYKGQCAEYCGGPHALMGFVVVAHSPEDFARWRASRV
                     PAGGGGAGLALFLSSGCAACHTIRGTPANGLAGPDLTHVGSRRTLGAGILPNNPGTMA
                     GWIADAQAIKPANRMPAYHQFDAAELGALTDYMVSLK"
     gene            complement(3171..>3410)
                     /locus_tag="VN034_05515"
     CDS             complement(3171..>3410)
                     /locus_tag="VN034_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWV60089.1"
                     /translation="CGACHTMPGIDWPKGRVGPALDGFGARAMIAGRLPNEAPLLAKY
                     VRDAPSLVPGSAMPAIPMTDAEARDIAAWLQSLRD"
BASE COUNT          698 a         1133 c         1132 g          447 t
ORIGIN      
        1 gccgacgcca acagcagcgc caccgaagcc gaaggccgcc accgcagcgc gccgcgaccc
       61 gcgagagcca ggaggaaggc cacgccataa aggagcagca ccgcagcggc ctcgccgagc
      121 gggggcggtg tcatccagaa agccggctga ttcgaccaaa ggaaggcgag gctgaagagc
      181 gccatgacga agatcatgcc catcacgacg agcaggatca ccatcgccca ccagccatgg
      241 ctcgacggcc ccgtcacata ggtcggaacc tcgatccccg cgccgatgtc gacgcgctcg
      301 gcctcgacgc gccggtcggt atcccacagc cagcgcagca ccgcgatgat cgcgagaacc
      361 ccgctcgccc acgcgaaagc ataggcctgg acagtgagcg cgaggaaaaa gcccgcggtg
      421 aagatcgccg cggcgaaggg ccatggcgac ggccccggca tgatctgcag atattgcggt
      481 tcggcaagga gcgggctcgt cacgatcgtc tcgcgcagcc ccgtcgccga gccgggcagg
      541 aaatagcgcc ccgccgcgac atcgtccgcg agggccggat cgtcccaaag cggttcgcgg
      601 ctcgtcacga ccgggatcga gcgcgtcgaa taaagctcgc ttggcagcca ttcgagcgtc
      661 ccgccgccgt aaacattgcc tgcattgccc tcgcccggcg cgagccggaa attgcgcgcg
      721 aggtcgacga gaaagagcag cacccccgcc ccgagcatga aggcgccgat cgtcgatatg
      781 agattgggaa gctcgagccc ctgccccggc agataggtat agacgcgccg cggcatgccc
      841 tggaggccgg cgaggtgcat cggcaggaaa gcgatgttca tgccggcgaa catcaggccg
      901 aacacccatt tggcgagccg ctccgacagc gcgcgccggc tgaccatcgg gatccagtaa
      961 tagaaggccg cgaagagcgg gaagaccatc ccgccgatca ggacataatg gagatgcgcg
     1021 acgatgaaat agctgtcgtg cgcctgccag tcgaagggca ccatcgcgac catcacgccg
     1081 gtgagcccgc cggtaacgaa aatgacgatg ctgccgagta tgaacagccc cggggcgttg
     1141 aaccgcggcg tccctaccgc gagcgtcgcg atccacgaaa agacctggat gcctgcggga
     1201 acgctcaccg ccatcgacgc ggccgagaag aagcccgtcg tcatcggcgg cattcccgtg
     1261 gtgaacatat ggtgcgccca gacgccgaag ctgatgaagc ccgtcgcgac catcgcgagc
     1321 acaatcagcc ggtagccgac gagttgctcg cgcgcgaccg ccgcgacgat catgctcatc
     1381 atgccggcag cgggaaggaa gatgatgtag acctcgggat gaccgaagaa ccagaagaga
     1441 tgctgccaga gcagtgggtc gccgccgcgc gtggcatcga agaagggcca attgaacgcg
     1501 cgctcgatct cgaggagcag ggtaccgaga atgatgctcg ggaaggcgat gacgatcatc
     1561 accgcgaaga cgagcatcgc ccaggcgaag atcggcatct tgtcgaggct catgcccggc
     1621 gcgcgggtgc gcagcacgcc gacgatgatc tcgaccgcgc cggcgatcgc gctgatctcg
     1681 atgaacccga tgccgagcaa ccagaagtcg gtgttgatcc ccggcgaata ggccttggac
     1741 gtcagcggcg gatacatgaa ccagccgccg tcgggcgcga ggccgacgaa gatcgaggcg
     1801 aagaagcaaa gcccgccgac cgcataggcc cagaaggcat aagccgacag gcgcgggaat
     1861 ggcaggtcgc gcgcggcgag catctgcggc aggagcagca cccccgccgc ctcgaccgcg
     1921 ggcaccgcga agaggaacat catcaccgtg ccgtgcatgg tgaagacctg attataggtc
     1981 ccctgcggca atatctcgag catcggcgcc gcgagctgcg cgcgcatcag gagcgccagc
     2041 acgcccgcga gtaggaagaa gagaaaagcg gtgccgagat aatagatgcc gacataatta
     2101 ttgttgacga cggtcagata ttcccagccc ttcggcttgc accagatgcg gcgaagcgcc
     2161 tcctcctcgc cctcgggacg cggtcccttg gtcgggaagc gatcgtagag cgcgggatcg
     2221 aaccccgtct ccgacctcat ttgaggctga ccatataatc ggtgagcgcg ccgagttcag
     2281 cggcgtcgaa ctggtgatag gcgggcatgc ggttggcggg cttgatcgct tgggcatcgg
     2341 cgatccagcc cgccatcgtg ccgggattgt tcggcaagat gcccgcgccc aaggtacggc
     2401 gcgagccgac atgcgtcagg tcgggtcccg cgaggccgtt cgcgggggtg ccgcggatcg
     2461 tatggcaggc ggcgcaaccg ctcgaaagga agagcgcgag ccctgccccg cccccgcccg
     2521 ccggaacgcg cgacgcccgc caacgcgcga aatcctcagg gctatgcgcg acgacgacga
     2581 agcccatcag cgcgtgcgga ccgccgcaat attcggcgca ctggcccttg tagacgcccg
     2641 gcgcatcggc ctcgatcagc aggcgattgg tccggccggg gatcatgtcc atcttgccgc
     2701 cgagccgcgg cacccagaag ctgtggatga cgtcggcggc ctcgagttcg agcagcaccg
     2761 gcgtgccgac cgggatatgc agctcgttcg catcgcgcaa cgtctcgcgg ccccggccat
     2821 cctgataatg cacgcgccac caccacattt cgccgatgat cttcacgcgc atcgcgcctt
     2881 cgggaatccg cgttgccgac agcgagctcg tcagcataag cccccagacg agcagcccgg
     2941 tgagcaccac ggaaggaaag gcaaagccaa ggatccagat cgccttctcg ccgccgagct
     3001 gggtcttgag ctttggcgaa ccgcgcagcg cgatccagag cgccgccagc acgatcgcga
     3061 acaccaccgt tccgccaatc agcagtgccc aggcgagcgt cgtcaccggc tcggaatagg
     3121 ggcccgcggg atcgagcacg ggcggcggcc agccggtttc gggaagccgg tcagtcacga
     3181 aggctctgga gccaggcggc gatatcgcgc gcctcggcgt cggtcatcgg gatcgcaggc
     3241 atcgcgctgc cggggacgag cgacggcgcg tcgcgcacat atttggccag caggggcgct
     3301 tcgttcggga gccgtcccgc gatcatcgcg cgcgcgccaa agccatccag cgccggaccg
     3361 acccgccctt tcggccaatc gattccgggc atcgtgtggc aggcgccgca
//
LOCUS       DATMSU010000214         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_174460,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000214 DATMSU010000000
VERSION     DATMSU010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174460"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..2081)
                     /locus_tag="VN034_05520"
     CDS             complement(78..2081)
                     /locus_tag="VN034_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWV60090.1"
                     /translation="MKSVSKLLVVAALLAGSASAAAYAQTGAPPPKKPANSTANTAGY
                     TAPGSTEGGPAYVGPIMTEWGRKVTPENAWRSYPRPQMERDTWMNLNGHWDYAIRPAN
                     EPQPAAMDGKILVPFAVESRLSGVARKLMPDDRLWYKRSFTLPAKWPGQRTLLHFGAV
                     DFESTVLVNGVVVGSHKGGSDPFSFDVTRYLRSGANELVVQVTDPTSTGDQPRGKQTL
                     EPRGIWYTAVSGIWQTVWLEPVADLHIADLRAVPDIDKGELTVDVALSNTATPSDAVR
                     ITARDKGKVIASTIIRGNRQGVLKIPNARLWSPDDPYLYDLTAQLVKVTPPQGAGNDR
                     TGLPPMTEREVAAYAAATVTGAPSDSVKSYFGMRKISTGIDPKTGKMALLLNNKPLFH
                     NGTLDQGWWPESLLTPPSEEAARSDIIFLKKAGFNMLRKHIKVEPAQYYHDADLLGIL
                     IWQDMPSGAFGDQAVRRPSDREATFTSAGMAQYQAELARMIGALRAFPSIVMWVTNNE
                     GWGQYDVKTVGSIAKNMDPSRLVNNASGWFDVPGSGSDVYDIHTYDEVPVEPAPQADR
                     PIVTGEFGGVGLPIPGHLWFTDRDARIYQFATDKEDYRRRYKIKFDEIIRQAKELGLA
                     ASVYTETSDVEGELNGLLTYDRAVSKLPVEDFAEIAKPLFEDK"
     gene            complement(2114..>2776)
                     /locus_tag="VN034_05525"
     CDS             complement(2114..>2776)
                     /locus_tag="VN034_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60091.1"
                     /translation="ATTLAAEGGTREHANPLRALGDFRVILLCIAFLGFPLSAYGLSY
                     WLPTIVRGFGVSDAVNGLLNIIPWLTVAAALWWLPRRAVRSAGSRRFMVGPALVGALS
                     LVLAVLLPAPPFQFAALCIAAAAIFAGQPFFWVLPGRFLTGAAAAAGFAAINSVGNLG
                     GFVAQAVVPKIHDATGSDLAPMFFLAAMLVFSAGAIFLIERHIPRGRSDGFPVATSSD
                     SL"
BASE COUNT          465 a          881 c          940 g          490 t
ORIGIN      
        1 catcagcgcc gcgagcatgg tggtcgcggc ccagcgggcc atcgcggtgc gtcgtgccat
       61 cggcgtttcc ccttgcctta tttatcctcg aacagcggct ttgcgatctc ggcgaaatcc
      121 tcgaccggca gcttcgacac cgctcggtca taagtgagga gaccgttgag ttcgccctcg
      181 acgtcgctcg tttcggtata gaccgacgcg gcgaggccca gttccttcgc ctgacggatg
      241 atctcgtcga acttgatttt gtagcggcgg cggtaatctt ccttgtcggt cgcgaactga
      301 tagatgcgcg catcgcggtc ggtgaaccac agatgccccg ggatcggcag cccgacgccg
      361 ccgaattcgc cggtcacgat cggccgatcg gcctgcggcg cgggttcgac gggtacttcg
      421 tcataggtgt ggatgtcata gacgtccgac ccgctgcccg ggacgtcgaa ccagccgctc
      481 gcattgttca cgaggcggct cgggtccata ttcttggcga tgctgccgac ggtcttgacg
      541 tcatactggc cccagccctc gttattggtc acccacatca cgatcgacgg aaaggcgcgg
      601 agcgcgccga tcatccgcgc cagttcggcc tgatattgcg ccatgccggc cgaggtgaag
      661 gtcgcttcgc ggtcgctcgg gcgccgcacc gcctgatcgc cgaacgcgcc cgagggcatg
      721 tcctgccaga tcaatatgcc gagcaggtcg gcgtcgtgat aatattgcgc gggttcgacc
      781 ttgatatgct tgcggagcat attgaagccc gcctttttca ggaagatgat gtcgctccgc
      841 gcggcttcct ccgacggcgg ggtgagcagg ctttcaggcc accagccctg gtcgagcgtg
      901 ccgttgtgaa agagcggctt gttgttgagc agcagcgcca tcttgccggt cttgggatcg
      961 atgccggtcg aaatcttgcg catcccgaaa tagcttttga cgctgtcgct gggcgcgccg
     1021 gtgacggtcg ccgcggcata ggccgcgacc tcgcgctcgg tcatcggcgg cagccccgtc
     1081 cggtcgttac ccgctccctg cggcggcgtc accttcacca gctgggcggt gaggtcatag
     1141 agataggggt cgtcgggcga ccagaggcgc gcgttcggaa tctttagcac gccctgccgg
     1201 ttgccgcgga tgatcgtgct cgcgatcacc ttgcctttgt cgcgcgcggt gatgcggacg
     1261 gcgtcgcttg gcgtcgcggt gttgctgagc gcgacatcga cggtcagctc gcccttgtcg
     1321 atgtcgggca ccgcgcgcag gtcggcgata tggaggtcgg cgacgggttc gagccacacc
     1381 gtctgccaga taccgctgac cgcggtgtac cagatgccgc gcggttcgag cgtctgtttg
     1441 ccgcgcggct gatcgccggt cgaggtcggg tcggtgacct gcacgacgag ctcgttggcg
     1501 ccggagcgca gatagcgggt gacgtcgaag ctgaacggat ccgagccgcc cttgtggctg
     1561 ccgacgacga cgccgttgac gagcacggtc gattcgaaat cgaccgcgcc gaagtggagc
     1621 aaggtgcgct ggcccggcca tttcgcgggc agcgtgaagc tgcgcttata ccagagccgg
     1681 tcgtcgggca tcagtttgcg cgcgaccccc gacagccgcg attcgaccgc gaaggggacg
     1741 aggatcttgc cgtccatcgc cgccggctgc ggttcgttcg cggggcggat cgcataatcc
     1801 caatgcccgt tgaggttcat ccaggtgtcg cgttccatct gcgggcgcgg atagctgcgc
     1861 cacgcatttt cgggcgtcac cttgcgaccc cactcggtca tgatcgggcc gacataggcg
     1921 ggcccgccct cggtgctgcc gggcgcggtg taacccgcgg tgttggcggt cgaattggcg
     1981 ggcttcttgg gcggcggcgc gccggtctgc gcataagcgg cggcgctggc gctgcccgcc
     2041 aagagcgcgg caaccacaag cagcttcgaa acagacttca tctgaccctc ccaatttata
     2101 tgatcaatgt gttttataaa gagtcgctcg atgtggcaac cggaaatccg tctgaacggc
     2161 cgcgcggaat atggcgctcg atcaggaaga tcgctcccgc cgaaaagacg agcatcgcgg
     2221 cgaggaagaa catcggcgcg aggtcgctgc cggtcgcgtc atggattttc ggcacgaccg
     2281 cctgcgcgac gaagccgccg agattgccga ccgaattgat cgcggcgaat ccggcggccg
     2341 ccgccgctcc ggtcaggaag cgtcccggca gcacccagaa aaagggctgc ccggcaaaga
     2401 tcgcggcggc agcgatgcac agtgcggcga actggaacgg gggcgccggc agcaacaccg
     2461 ccagcacgag gcttagcgca ccgacgagcg ccggcccgac catgaagcgg cgcgacccgg
     2521 cgctccgcac cgcccggcgc ggaagccacc acaaagccgc cgcaacggta agccagggga
     2581 tgatgttgag caggccattg acggcgtcgc tgacgccgaa accccggacg atcgtcggaa
     2641 gccaatagct gaggccatag gccgacagcg gaaagccgag gaaagcgatg cagagcagga
     2701 tcacgcgaaa atcgccgagc gcgcgcagcg gattggcgtg ctcgcgcgtg ccgccctctg
     2761 cggcgagcgt cgtggc
//
LOCUS       DATMSU010000215         1652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_155623,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000215 DATMSU010000000
VERSION     DATMSU010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155623"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VN034_05530"
     CDS             <1..720
                     /locus_tag="VN034_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HWV60092.1"
                     /translation="GLHTRLTSIPALVEAARRTGQRLIDVRTPPPSIPIATGRKRSGK
                     RLLTVGTDCALGKKYTALALHRAFVARGIDADFRATGQTGIMIAGGGMPMDAVVSDFE
                     AGAAEILSPDAPDNHWDLVEGQGSIFNPAYAAVSLGLLHGSQPDVFVVCHDPARKVIL
                     GMESFALPSIEEVIDLTIRLGSRTNPAIRCGGVSLNTSAYDADAAEALMAAERQRLGL
                     PVTDPIRGGASFDALVDNILA"
     gene            717..>1652
                     /locus_tag="VN034_05535"
     CDS             717..>1652
                     /locus_tag="VN034_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60093.1"
                     /translation="MSVGETGGRSSWFQRFLLPGFALKAVIIGGGYATGRELAEYFVP
                     SGPWGGLSAMLLATLIWSIVAALTFALARQLGAYDYRAFFKGLLGPAWVAFEAAYLIF
                     VILILAVFGAAAGAIGAATFGWPELAGVVLLAVSIVAVTAWGTGVVEQMFKYVSILLY
                     TVYGLFLILALASFGGLIGQGFANAPPPSGNWMAGGLTYASYNIVGAVVILPVLRHLT
                     SQRDAVVAGLIAGPLSMLPAILFFVAMMAFYPAIGDETLPSDFLLRQMTVPGFHILFQ
                     VMIFAALLESGAGAVHAVNERISGAVESRGRPPLST"
BASE COUNT          219 a          560 c          562 g          311 t
ORIGIN      
        1 ggcctccaca cgaggctcac gagcatcccg gcgctcgtcg aggcggcgcg gcgcaccggc
       61 cagcggctga tcgacgtgcg caccccgccg ccgtcgatcc cgatcgccac cgggcgcaag
      121 cggagcggca agcgcctgct gaccgtcggc accgattgcg cgctcggcaa gaaatatacc
      181 gcgctggcgc tgcaccgcgc ctttgtcgcg cgcgggatcg acgccgattt ccgcgcgacc
      241 ggccagaccg ggatcatgat cgccggcggc ggcatgccga tggacgcggt ggtttcggat
      301 ttcgaggccg gggcggccga aatattgagc cccgacgcgc ccgacaatca ctgggatctg
      361 gtcgaggggc aggggtcgat cttcaacccc gcctatgccg cggtgtcgct cggcctgctc
      421 cacggcagcc agcccgatgt cttcgtcgtc tgccacgacc cggcgcgcaa ggtgatcctc
      481 ggcatggaga gctttgcgct ccccagcatc gaggaggtga tcgacctgac gatccgcctc
      541 ggtagccgga ccaatcccgc gatccgctgc ggcggcgtga gcctcaacac ctcggcctat
      601 gacgccgacg cggccgaagc gttgatggcg gctgagcgcc agcggctcgg cttgccggtg
      661 accgatccga tccgcggcgg cgcgtcgttc gacgcgctcg tggacaatat tctcgcatga
      721 gtgtgggcga gacgggcggc aggtcgagct ggttccagcg tttcctgctg cccggttttg
      781 cgctgaaggc cgtgatcatt ggcggcggct atgcgacggg gcgcgaactc gccgaatatt
      841 tcgtgccgtc gggcccgtgg ggcgggctgt ccgcgatgct gctcgcgacc ctgatctgga
      901 gcatcgtcgc cgcgctgacc ttcgcgctcg cgcgccagct cggcgcctat gattatcgcg
      961 cctttttcaa aggacttctc ggccccgcct gggtcgcctt cgaggcggcc tatctgatct
     1021 tcgtcatcct gatcctcgcg gtgttcggcg ccgcggcggg cgcgatcggg gctgcgacct
     1081 tcggctggcc ggaactggcg ggcgtcgtgc tgctcgccgt ctcgatcgtc gcggtgaccg
     1141 cttgggggac cggcgtcgtc gagcagatgt tcaaatatgt ctcaatcctc ctctacaccg
     1201 tctatggtct gttcctgatc ctcgcgctcg cgagtttcgg cgggctgatc gggcaaggct
     1261 tcgcgaacgc gccgccgcct tcgggcaact ggatggcggg cgggctgacc tatgcgagct
     1321 ataatatcgt cggcgcggtg gtgatcctgc cggttctgcg gcatctgacc agccagcgcg
     1381 acgccgtggt cgccggcctg atcgcgggac cgctctcgat gctgcccgcg atcctcttct
     1441 tcgtcgcgat gatggccttt tatccggcga tcggcgatga gactttgccg tcggacttcc
     1501 tcctccgcca gatgacggtg ccgggcttcc acatcctgtt ccaggtgatg atcttcgccg
     1561 cgctgctcga aagcggcgcg ggcgcggtgc atgcggtgaa cgagcgtatc tcgggcgcgg
     1621 tcgaaagccg cggacgcccg ccgctctcga cc
//
LOCUS       DATMSU010000216         3466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_155657,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000216 DATMSU010000000
VERSION     DATMSU010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155657"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VN034_05540"
     CDS             complement(<1..816)
                     /locus_tag="VN034_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV60094.1"
                     /translation="MLTINGLTVRLGGRTILDRAAASLPGKSRTGLIGRNGAGKSTLM
                     KVMIGQLEADEGGIEMPRKTRVGYIAQEAPSGSATPFETVLAADTERAELLAASEVEQ
                     DAHKLGDIHERLIAIDAYAAPARAARILIGLGFDEEMQGQPLDSFSGGWKMRVALAAL
                     LFSNPDLLLLDEPSNHLDLEATMWLESFLRAYPGQLVVISHERDLLNNVVDHILHLEG
                     GKVTLYPGGYNDFERQRAERIAQLAAAKAAQDAQRAKLQDYVARNSARASTAKQ"
     gene            1051..1473
                     /locus_tag="VN034_05545"
     CDS             1051..1473
                     /locus_tag="VN034_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV60095.1"
                     /translation="MADLAPFHIAFPVDDLDAARHFYGGVLGCPEGRSDADWIDFNLY
                     GHQIVAHRVDTPRTRPAHNPVDGHDVPVPHFGVVLPPAEWQALAERLKAAGTEFVIEP
                     HTRFPGQPGEQSTMFFHDPAGNALEFKAFADLGQLFAR"
     gene            complement(1477..3027)
                     /locus_tag="VN034_05550"
     CDS             complement(1477..3027)
                     /locus_tag="VN034_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWV60096.1"
                     /translation="MASAAAPASAIPSEPQPLSGVRLIVAAFALALANFVVVLDTTIA
                     NVSVPHIAGGLAVSPTQGTWVITSYAVADAISVPLTGWLAMRFGTVRWFMISIIGFGI
                     FSFLCGVSRTLDALVLFRVLQGLAGGPLMPLSQILLLRIFPKEKAGIGLAIWAMTTTT
                     APILGPILGGTISDNWTWPWIFFINLPVVAICAFGVFTLLTPFETKRAKARIDIVGLI
                     LLVVSVGAFQIMLDTGREHDWFGSVWIVGLAIVAAISFAAFVIWELTDANPVVNLRIF
                     RFRGFTFATMAISFGFGAFFAQVVLTPLWLQQVAGYTATETGFIVAWLGVFAVLLSPV
                     AAGLMTKVDVRLTISAGILWMAVMSVLRASWNADVGYWTLALPHILQGIGMPFFFVGL
                     TALALSSVPAQHQTSAAGLMSFLRTLCGAIGTAIATTAWDDATRTSRSELVSSLNNPE
                     ATMTSLQNAGFTLEQARAAIERLVEVQASTLGVIHLFLASGVVFVIAAISVWFAPRPK
                     QISMGGGH"
     gene            complement(3032..>3466)
                     /locus_tag="VN034_05555"
     CDS             complement(3032..>3466)
                     /locus_tag="VN034_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWV60097.1"
                     /translation="EKARLDLARTVLRAPVDGIVTNRAVQVGQRIAAGAPIMVIVPIA
                     TAYVDANFKESQFGDIRIGQPVELTSDYYGGDVVYRGKVVGIAGGTGAAFSLIPAQNA
                     TGNWVKVVQRLPVRIALDPKQLKAHPLRVGLSMEATIDTRGN"
BASE COUNT          614 a         1143 c         1164 g          545 t
ORIGIN      
        1 ctgcttcgcg gtcgaggcgc gcgcgctgtt gcgcgcgaca taatcctgca gcttggcgcg
       61 ctgcgcatcc tgcgccgcct tggcggcggc gagctgcgcg atccgttcgg cgcgctggcg
      121 ttcgaaatca ttatagccgc cgggatagag cgtcaccttc ccgccctcga ggtgaaggat
      181 atggtcgacg acattgttca agaggtcgcg ttcgtggctg atcacgacga gctggcccgg
      241 ataggcgcgc aaaaagcttt cgagccacat cgtcgcttcg aggtcgaggt ggttcgacgg
      301 ttcgtcgagc agcaagaggt cggggttcga gaaaagcaag gccgcgagcg cgacgcgcat
      361 cttccacccg cccgagaagc tgtcgagcgg ctggccctgc atctcctcgt cgaagcccaa
      421 tccgatcagg atgcgcgcgg cgcgcgcggg cgcggcatag gcgtcgatcg cgatcagccg
      481 ttcgtggatg tcgccaagct tgtgcgcgtc ctgctcgacc tccgacgccg cgagcagttc
      541 ggcgcgttcg gtgtcggcgg cgagcacggt ttcgaacggc gtcgcgctgc cgctcggcgc
      601 ttcctgcgcg atatagccga cgcgcgtctt gcgcggcatt tcgataccgc cctcgtcggc
      661 ttcgagctgg ccgatcatca ccttcatcaa ggtcgacttg cccgcgccgt tgcggccgat
      721 gaggccggtg cggcttttgc ccggcaggct cgcggccgcg cggtcgagga tggtgcgccc
      781 gccgaggcgc acggtgaggc cattgatcgt cagcatgcgc gcgcgcctag catgttcggc
      841 gccgcggccc aaggggcgcc ttgatgtcga gaccgtcaca cagaatatcg agccatagtc
      901 ccgcgtcccc gagcgaagtc gaggggctct gccgagcgaa gtcgaggctg cgacgatgca
      961 ggttctcgct ccgctcgaac gggcccctcg acttcgctcg gggacacggg ggaggtttca
     1021 ggttttgccc cactgctata gagatcgcac atggccgatc tcgccccctt ccatatcgcc
     1081 tttccggtcg acgacctcga cgccgcgcgc catttctatg gcggcgtcct cggctgcccc
     1141 gaggggcgga gcgatgccga ctggatcgac ttcaacctct acggccacca gatcgtcgcg
     1201 caccgcgtcg acacgccgcg cacgcggcct gcgcataatc cggtcgacgg ccatgacgtt
     1261 ccggtgccgc atttcggcgt cgtgctgccg cctgccgaat ggcaggcgct cgccgagcgg
     1321 ctgaaggcgg cgggcaccga gttcgtgatc gaaccacata cgcgctttcc cggccagccc
     1381 ggcgaacagt cgacgatgtt cttccacgac cccgcgggta atgcgctcga attcaaggcc
     1441 ttcgccgacc ttggtcagct gttcgctcga taagggctaa tgcccgccgc ccatcgaaat
     1501 ctgcttgggc cgcggcgcga accataccga gatggcggcg atcacgaaca ccacgcccga
     1561 cgcgaggaag aggtggatga cccccaatgt cgaggcctgc acctcgacca gccgctcgat
     1621 cgccgcgcgc gcctgttcga gcgtgaagcc ggcgttttgc agcgaggtca tcgtcgcttc
     1681 gggattgttg agcgaggaga cgagttcgga gcgcgaggtg cgggtcgcgt cgtcccatgc
     1741 ggtcgtcgcg atcgcggtgc cgatcgcgcc acatagcgtg cgcaggaagc tcatcagccc
     1801 cgccgccgac gtctggtgct gcgcgggcac cgagctcagc gcgagcgcgg tgagcccgac
     1861 aaagaaaaag ggcatgccga tcccctgcag gatatgcggc agcgcgagcg tccagtaacc
     1921 gacgtcggcg ttccagctcg cgcgcaggac cgacatcacc gccatccaga ggatgcccgc
     1981 cgagatggtg aggcgcacgt cgaccttggt catcaggccc gccgccacgg gcgacagcag
     2041 caccgcgaac acgccgagcc aggcgacgat gaaacccgtc tcggtcgcgg tataacccgc
     2101 aacctgctga agccacaaag gcgtcagcac gacctgcgcg aagaaggcgc cgaagccgaa
     2161 cgagatcgcc atcgtcgcga aggtgaagcc gcggaagcgg aagatgcgca gattgacgac
     2221 cggattggcg tcggtcagct cccagatgac gaaggcggca aagctgatcg cggcgacgat
     2281 cgcgagcccg acgatccaga ccgagccgaa ccagtcgtgc tcgcggccgg tgtcgagcat
     2341 gatctggaac gcaccaaccg acacgacgag caggatcagg ccgacgatgt cgatgcgggc
     2401 cttggcgcgc ttggtttcga acggcgtcag cagcgtgaac accccgaacg cgcagatcgc
     2461 gacgacgggc agattgatga agaagatcca cggccaggtc caattgtcgc tgatcgtgcc
     2521 gccgaggatc gggccgagga tcggcgccgt ggtcgtcgtc atcgcccaga tagcaaggcc
     2581 gatccccgcc ttttccttgg gaaagatacg cagcagcaag atttgcgaca gcggcatcag
     2641 cgggccgccc gcgaggccct ggagcacgcg gaagagcacc aaggcgtcga gcgtgcgcga
     2701 gacgccgcag aggaaggaga agatgccgaa gccgatgatc gagatcatga accagcgaac
     2761 ggtgccgaag cgcatcgcga gccagccggt gagcggcacg ctgatcgcat cggcgaccgc
     2821 atagctggtg atcacccatg tcccttgcgt cggcgacacc gcgagcccgc ccgcgatatg
     2881 cggcaccgac acattggcga tcgtcgtgtc gagtacgacg acgaaattgg cgagcgcgag
     2941 cgcgaacgcc gcgacgatca gccgcacgcc gctgagcggt tgcggctcgg aagggatcgc
     3001 ggaggcgggg gcggcggcgc tggccatgat gtcagttccc gcgcgtgtcg atcgtggctt
     3061 ccatcgacag cccgacgcgc agcggatgcg ctttcagctg ctttggatca agcgcgatgc
     3121 gtaccggcag gcgctggacg accttcaccc agttgcccgt cgcattctgc gccgggatca
     3181 gcgagaaggc cgcgcccgtg ccgcccgcga tgccgacgac cttgccgcga tacacgacgt
     3241 cgccgccata atagtccgac gtcagctcga ccggctggcc gatccggata tcgccgaact
     3301 ggctttcctt gaaattcgca tcgacatagg cggtcgcgat cggaacgatg accatgatcg
     3361 gcgcgcctgc ggcgatccgc tgccccacct gcaccgcgcg gttggtgacg atgccgtcga
     3421 ccggcgcgcg caggacggtg cgggcgaggt cgagccgcgc cttttc
//
LOCUS       DATMSU010000217         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_4173, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000217 DATMSU010000000
VERSION     DATMSU010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4173"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VN034_05560"
     CDS             <1..1165
                     /locus_tag="VN034_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWV60098.1"
                     /translation="ETLALLDPASQAPAGPPPRTNRHLYDEANPLDLIPFAKKVELCQ
                     KVDAAARARDPRVVQVSVALAGSWSVVEIVRADGFLATDIRPLVRLNVSIVVEENGRR
                     ESGYFGLGGRYMYDHLFEEAQWNRAIDEALNQALVNLRAVDAPAGEFTVLLGPGWPGV
                     LLHEAVGHGLEGDFNRKGTSAFSGRIGERVAAPGVTVVDDGAMESPVGGGRRGSLSID
                     DEGTPTGETVLIEDGILKGYMQDRLNARLMGVEPTGNGRRESFAHAPMPRMTNTFMRG
                     GGDDPAELLSRVKNGIFAKSFGGGQVDIVSGKFVFSCTEAYKIENGKIGDSIKGATLI
                     GDGPSVLTKVTGIGNDMAIDEGIGICGKAGQSVPAGVGQPTLLVSGLTVGGTA"
     gene            1466..2683
                     /locus_tag="VN034_05565"
     CDS             1466..2683
                     /locus_tag="VN034_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60099.1"
                     /translation="MRSTLHFVSAAAVAAMLAAVPGSAQKTTGPKERYEMDVATMSGF
                     AAMSGGRGGLGGAMGIMFGGDPSNKVAHQLLLRLGSNDAATAPPPKGDHFFQPQAKMG
                     KSVPLYWEEGKEETGTTPFERPKGRLLLYWGCGAKAGPGQPVVIDFAKVAAGQMPPNL
                     FSSAVPVIREVSKANSRSYIDWPNKKGGRQPGSGSSLLGAHRVASNIGPDINFTLAQD
                     YMQGLSASTAIQGDQSVMVRWNAVPGATGYVAWTIGGMSGGNGGNDMGDIVWWTSSAS
                     KEFGGGLWDWLPPSVVANLITKKIVMPPAQTSCQIPAEVKKASGEMLIGNLNAFGPEA
                     NFSYPPKPAGNAAWNIDWTAKVRFRSHTMLMVGADFGGMSGANTGGSTPAEPAKKKKC
                     KGPFGIPLPDGAC"
     gene            2766..3167
                     /locus_tag="VN034_05570"
     CDS             2766..3167
                     /locus_tag="VN034_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60100.1"
                     /translation="MRHLIPALIAAAAVAAAPAATAREKLEPEAELAKLLEGRVAGEP
                     QDCISLSTARSSQIIDKTAIVYKAGSTYWVNRPRGGAASLDDDDILVTRLTGSQLCSI
                     DTVELRDRASRMYSGFVSLGEFVPYRHAKGE"
     gene            3304..>3614
                     /locus_tag="VN034_05575"
     CDS             3304..>3614
                     /locus_tag="VN034_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HWV60101.1"
                     /translation="MAEKEYAPPPADWAQQLLAFWFDEHGMEDWYGGGPDFDAEVRDL
                     AEGWHEALRSQPAEAFLTDPDTALAATILFDQVPRNIYRGHADAFATDSLARAIARGI
                     VA"
BASE COUNT          647 a         1178 c         1224 g          565 t
ORIGIN      
        1 cgagacgctc gccttgctcg atcccgccag ccaagccccc gccggcccgc cgccgcgcac
       61 caaccgccac ctttacgacg aggcgaaccc gctcgacctc atccccttcg cgaagaaggt
      121 cgagctttgc cagaaggtcg acgctgccgc gcgcgcgcgc gatccgcgcg tcgtgcaggt
      181 ctcggttgcg ctcgcgggca gctggtcggt ggtcgagatc gtccgcgccg acggctttct
      241 cgcgaccgac atccgcccgc ttgtccgcct caacgtctcg atcgtggtcg aggagaatgg
      301 gcgccgcgaa tcgggctatt tcggcctcgg cgggcgctac atgtacgacc atctgttcga
      361 ggaggcgcag tggaaccgcg cgatcgacga ggcgctgaac caggcgctcg tcaacctgcg
      421 cgcggtcgac gcgcccgcgg gcgagtttac cgtgctgctc ggccccggct ggcccggcgt
      481 gctgcttcat gaggcggtcg gccacgggct ggagggcgat ttcaaccgca agggcaccag
      541 cgccttttcg ggcagaatcg gagagcgcgt cgcggcgccg ggtgtcacgg tggtcgacga
      601 cggcgcgatg gaaagccccg tgggcggcgg ccgccgcggt tcgctcagca tcgacgacga
      661 aggcaccccg accggcgaga cggtgctgat cgaggacggt atcctcaagg gttatatgca
      721 ggaccgcctc aacgcgcgcc tgatgggggt cgaaccgacg ggcaacggac ggcgcgagag
      781 tttcgcgcac gcgccgatgc cgcggatgac caacaccttc atgcgcggcg ggggcgacga
      841 tcccgccgaa ttgctgagcc gcgtcaagaa cggcatcttc gccaagagct tcggcggcgg
      901 acaggtcgat atcgtctcgg gcaagttcgt cttcagctgc accgaggcct acaagatcga
      961 gaatggcaaa atcggcgact cgatcaaggg cgcgactttg atcggcgacg ggccgagcgt
     1021 gctgaccaag gtcaccggga tcggtaacga catggcgatc gacgagggca tcggcatctg
     1081 cggcaaggcg gggcagagcg tccccgcggg cgtcggccag ccgaccttgc tggtgagcgg
     1141 gctgacggta ggcgggacgg cgtaagttcc ttctcccttg atgggagaag gatacgcagc
     1201 cttgccgcga agcggctagg cgaagttgga tgagggtgac ggcgcgccat caaaccatcg
     1261 cttcagaccg agaccacacc cccaccgctg cgactagcca gcaagctggc aagtctcgct
     1321 gcctccccca tcaaggggga ggttgatcgc cctgtgatcg ccctcacttg gcgtcccggc
     1381 ccgccgcgca tataggatag ccaaacagaa agaagattcg gtcgcatcat ccgtgggggg
     1441 gatcccctgc aacagggagg atagtatgcg ttcgactctg cattttgtgt ccgcggccgc
     1501 cgtcgccgcg atgctcgccg ccgttcccgg ctcggcgcaa aaaacgaccg gccccaagga
     1561 acggtacgag atggatgtcg cgacgatgtc gggcttcgcc gccatgtcgg gaggccgcgg
     1621 cgggcttggc ggcgcgatgg ggattatgtt cggcggcgat ccgtcgaaca aggtcgcgca
     1681 tcagctcctg cttcgcctcg gctcgaacga cgccgccacc gcgccgccgc cgaagggcga
     1741 ccatttcttt cagccgcagg cgaagatggg caagtcggtc ccgctatatt gggaagaggg
     1801 caaggaagag accggaacga cgccgttcga gcgcccgaag ggccgcctgc tgctctattg
     1861 gggctgcggc gcgaaggcgg ggccggggca accggtggtg atcgacttcg ccaaggtcgc
     1921 cgcgggccag atgccgccga acctgttttc gagcgcggtg ccggtgatcc gcgaggtcag
     1981 caaggcgaac agccgttcct atatcgactg gccgaacaag aagggtgggc gccagccggg
     2041 atcgggctcg tcgctgctcg gcgcgcaccg cgtcgcgagc aatatcggcc ccgacatcaa
     2101 tttcacgctc gcgcaggatt atatgcaggg gctgtcggcc tcgaccgcaa tccagggcga
     2161 ccagtcggtg atggtgcgct ggaatgcggt gccgggcgcg acgggctatg tcgcgtggac
     2221 gatcggcggc atgtcgggcg gcaacggcgg gaacgacatg ggcgacatcg tctggtggac
     2281 gagcagcgcg tcgaaggaat tcggcggcgg cctgtgggac tggctgccac cgtcggtcgt
     2341 cgcgaacctg atcaccaaaa agatcgtgat gccgcccgcc cagacgagct gccagatccc
     2401 cgccgaggtc aagaaggcgt cgggcgagat gctgatcggc aatttgaatg ccttcggtcc
     2461 cgaagccaat ttctcctacc cgccgaagcc cgccggcaat gcggcgtgga acatcgactg
     2521 gaccgcgaag gtgcgtttcc gttcgcacac gatgctgatg gtcggcgccg atttcggcgg
     2581 tatgagcggc gcgaacacgg gaggcagcac ccccgccgag cccgcgaaaa agaagaagtg
     2641 caagggcccg ttcgggatcc cgctccccga cggggcgtgc tgaaacggcg gcgagtcagc
     2701 ccctgttcac gccaagtggc gtaaaagggc gccgggtagg gtatgatgaa gacaggagtc
     2761 gaaccatgcg tcacctcatc ccggcgctca ttgccgcggc ggccgtagcg gccgcccccg
     2821 ccgctaccgc gcgcgaaaag ctcgagcccg aagccgagct tgccaagctg ctcgaaggcc
     2881 gcgttgcggg cgaaccgcag gactgtatct cgctctcgac cgcacgcagc tcgcagatca
     2941 tcgacaagac cgctatcgtc tacaaggccg gcagcactta ttgggtcaac cggcccaggg
     3001 gcggcgccgc gtcgctcgac gacgacgata tcctcgtcac caggctgacc ggcagccagc
     3061 tgtgcagcat cgacaccgtc gaactgcgcg accgggcgag ccggatgtac agcggcttcg
     3121 tctcgctcgg cgaattcgtg ccgtatcgcc acgcgaaggg cgaatgatat cgggcggttg
     3181 cgcccaattt gatcgaagct aaattcgtca tcccggcgaa ggccgggatc tccatgttgc
     3241 gtcaaaccgt tacgtcgaga tcccggccct tcgccgggat gacgctttga tgggaacaat
     3301 gttttggccg aaaaagaata tgcccctccc cccgccgact gggcgcagca actgctcgcc
     3361 ttctggttcg acgaacatgg catggaggac tggtacggcg gcggccccga tttcgatgcc
     3421 gaggtccgcg accttgccga aggctggcac gaagcgctcc gctcgcagcc cgccgaggcc
     3481 tttctgaccg atcccgacac cgcgctcgcg gcgacgatcc tcttcgatca ggtgccgcgc
     3541 aacatctatc gcggccacgc cgacgccttc gcgaccgaca gcctcgcccg cgcgatcgcg
     3601 cgcggcatcg tcgc
//
LOCUS       DATMSU010000218         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_183833,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000218 DATMSU010000000
VERSION     DATMSU010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183833"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VN034_05580"
     CDS             <1..731
                     /locus_tag="VN034_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HWV60102.1"
                     /translation="SHAEGDPRVQDPYCLRCQPQVMGAALDLLRQAGTTLGIEANGVS
                     DNPLIFADTDEALSGGNFHAEPVAFAADMIAMALCEIGSIAERRIAMLVDPALSGLPA
                     FLTPRPGLNSGFMIPQVTAAALVSENKQRAYPASVDSIPTSANQEDHVSMAAHGARRL
                     LDMADNASAVIGIELLAACQGIDFHAPLKSSDALEAAHEHLRAAVPTLEDDRHFHPDM
                     EAATALIRSGGLVAAVPASLPGLG"
     gene            731..1534
                     /gene="hutG"
                     /locus_tag="VN034_05585"
     CDS             731..1534
                     /gene="hutG"
                     /locus_tag="VN034_05585"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0019557 - histidine catabolic process to
                     glutamate and formate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate deformylase"
                     /protein_id="HWV60103.1"
                     /translation="MDWLRVHRGDAPLVIAFPHGGTHLAGLDEQFVSPWHAQIDTDWW
                     IADLYAFAADLGATLVATDISRSVIDMNRDPSGASLYPGQATTELCPTTTFDGEPLYR
                     FGEPDEASITQRLNLYHRPYHDTLAAELDRLKAAHGKVVLYDAHSIRSHVPRLFDGEL
                     PQFNIGTNGGATAAPELETIVANICAASGKSHVVNGRFKGGWTTRHYGRPDDGIHAIQ
                     MELAQRGYMAEPDAITHTNWPSPLDPNPAIRPVLEQVIAATLDFAKGHS"
     gene            1531..>2075
                     /locus_tag="VN034_05590"
     CDS             1531..>2075
                     /locus_tag="VN034_05590"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067094.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWV60104.1"
                     /translation="MTARPLNQRLDNSRVIRPATGPEISAKSWLTEAPMRMLMNNLHP
                     DVAEAPHELVVYGGIGRAARDWESYDRIVETLKRLEGDETLLIQSGKPVGVFRTHENA
                     PRVLLANSNLVPEWANWEHFHELDKKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEM
                     GRQHYGGSLAGRWLLTAGLGG"
BASE COUNT          358 a          743 c          646 g          328 t
ORIGIN      
        1 cctcgcatgc ggagggcgat ccgcgcgtgc aggaccccta ttgcctgcgc tgccagccgc
       61 aggtgatggg cgccgcgctc gacctgctgc ggcaggccgg aacgacgctc ggcatcgagg
      121 cgaacggcgt ctcggacaat ccgctgatct tcgccgacac cgacgaggcg ctgtcgggcg
      181 gcaatttcca cgccgagccc gttgccttcg ccgccgacat gatagcgatg gcgctgtgcg
      241 agatcggctc gattgccgag cgccgcatcg cgatgctcgt cgatcccgcg ctgtcgggcc
      301 tccccgcctt cctcaccccg cgccccgggc tcaactcggg cttcatgatc ccgcaggtca
      361 ccgccgccgc gctggtcagc gagaacaagc agcgcgccta tccggcgagc gtcgattcga
      421 tcccgacctc ggccaatcag gaggaccatg tctcgatggc cgcgcacggc gcgcgtcgcc
      481 tgctcgacat ggccgacaat gccagcgcgg tgatcggcat cgaattgctc gcggcgtgcc
      541 agggcatcga tttccacgcg ccgctcaaat cgagcgacgc gctcgaagcc gcgcacgagc
      601 atcttcgcgc cgcggtgccg acgctcgagg acgaccggca tttccacccc gatatggaag
      661 ccgcaaccgc gctgatccgc tcgggcggcc tcgtcgccgc ggtgcccgcc agcctgccgg
      721 gtctcggctg atggactggc tgcgcgtcca tcgcggcgac gcgccgctcg tcatcgcctt
      781 cccgcatggc ggcacccacc ttgcgggact cgacgagcag ttcgtctctc cctggcacgc
      841 gcagatcgat accgactggt ggatcgccga tctttacgcc ttcgccgccg atctgggtgc
      901 gactttggtc gcgaccgaca tttcgcgcag cgtgatcgac atgaaccgcg atccgtcggg
      961 tgcgtcgctc tatccggggc aggcgacgac cgaactatgc ccgacgacga ccttcgacgg
     1021 cgagccgctc tatcgcttcg gcgagcccga cgaggcgtcg atcacccagc ggcttaacct
     1081 ctatcaccgc ccctatcacg acacgctggc ggccgaactc gaccggctga aggccgcgca
     1141 cggcaaggtc gtgctctacg acgcgcattc gatccgcagc catgtcccgc gcctgttcga
     1201 cggcgagctg ccgcagttca acatcggtac caacggcggc gcaaccgcag cgcccgaact
     1261 cgaaaccatc gttgccaaca tctgcgccgc aagcggcaag agccatgtcg tcaacggccg
     1321 tttcaagggc ggctggacga cgcgccacta tggccgcccc gacgacggca tccatgccat
     1381 ccagatggag ctggcgcagc gcggttatat ggccgagccc gatgcgatca cgcacacgaa
     1441 ttggccctcc cccctcgacc cgaacccggc gatccggccc gtgctggagc aagtgatcgc
     1501 cgcgaccctt gactttgcga aaggacattc atgaccgccc gccccctcaa tcagaggctc
     1561 gataacagcc gcgtgatccg cccggcgacc ggccccgaga tcagcgcgaa gagctggctc
     1621 accgaagcgc cgatgcggat gctgatgaac aacctccacc ccgacgtcgc cgaggcgccg
     1681 cacgagctcg tcgtctatgg cggtatcggc cgcgccgcgc gcgactggga aagctatgac
     1741 cggatcgtcg agacgctgaa gcggctcgag ggcgacgaaa cgctgctgat ccagtcgggc
     1801 aagcccgtcg gcgtgtttcg tacgcacgag aatgcgccgc gcgtcctgct cgccaattcg
     1861 aacctcgtcc ccgaatgggc gaattgggag catttccacg agctcgataa aaaggggctg
     1921 atgatgtacg gccagatgac ggccggaagc tggatctaca tcggcagcca aggcatcgtt
     1981 cagggcactt acgaaacctt cgtcgaaatg ggccgccagc attatggcgg cagccttgcg
     2041 ggccgctggc tgctcaccgc gggtctcggc ggcat
//
LOCUS       DATMSU010000219         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_98620, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000219 DATMSU010000000
VERSION     DATMSU010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98620"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..461
                     /locus_tag="VN034_05595"
     CDS             297..461
                     /locus_tag="VN034_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60105.1"
                     /translation="MRWALEVAGRRRQARKTRFARTLDKSVTVILRFDVGRVTNASFL
                     RNLGMIKRRA"
     gene            458..1486
                     /locus_tag="VN034_05600"
     CDS             458..1486
                     /locus_tag="VN034_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase"
                     /protein_id="HWV60106.1"
                     /translation="MKTTDLAPTRLPDHLRETQRLLFIAKHARWTGGLHPDDGNHAPY
                     HREMRETLEGLGTALSIADSYAALFDRPAVDLVFPLLNRGGFFNSEMLVPLLCNRHGL
                     PYLGASPIVRGLSDDKHLTKLEAAARGVPTAPWALFRRGAPVDTARCPPARRWVIKPN
                     NSSASWGVRDAHDRGQLAQAIFEIHALDHDAIVEPFIDGSDIEVPVVTLNGEPAMLPM
                     MIFEQADPTQLRTYQEKRDLVGDVGYAVKPFDDPFIAGRVADYTARMADVFRPFDYGR
                     FEFRFDHATGEVRLLEVNLNCNLWSEKLFSRSAILAGFTHAELIETIVTESMLRQIAP
                     AAARRDAA"
     gene            1483..2343
                     /locus_tag="VN034_05605"
     CDS             1483..2343
                     /locus_tag="VN034_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWV60107.1"
                     /translation="MSGSILILAGRRAGAADPLANTHGVADKCLVPVAGRPMIAHVLD
                     SAAASAATHIFVSTHHADLLGDLDDPILKSLGDRLIIVPAADNLADSVLAVAGLAEFP
                     LLVTTADNCLLTPATIAEIEADAIRRGVDAGVALARREDVLAVHPEGQRRFYAFSDVS
                     VSNCNAYWIGHPGALKAAEAFRGGGQFVKKPLRVIRAFGLINLLRFRFGLGPIHHVFA
                     RISRHLKVEIAPLLISDGATAIDVDNERSLRVTEALMARPDRINPPPNCHENPEEPGA
                     AGERRLALTR"
     gene            complement(2264..3706)
                     /locus_tag="VN034_05610"
     CDS             complement(2264..3706)
                     /locus_tag="VN034_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWV60108.1"
                     /translation="MASNVETLIRSAVAKGIGKVADFNRKRLPRPTDAHPFLTGIHKP
                     MTEEVTLEELRVDGEIPAQLKGRYLRNGPNPAVAPDPASYHWFTGAGMVHGIRIEGGK
                     ADWYRNRWVRGSEACEMLGEELPPGPREGRNDAPNTNVVGLAGRTFAIVEAGGKPVEL
                     GYELNTFAHNPFDGTLAGAYTAHPHHDPFSGEMHAITYRGDEPNKVWHVVLDKDAHVI
                     REEAIAVSDGPSIHDCAITENYVLVFDLPVTFSMKMLLAGYRFPYAWNDAHPARVGLL
                     PRNGSGDSIAWVPVEPCYVFHPANAFETADGKVIVDVVAHETMFAESKRGPDSQKSRM
                     ERWTIDPAARTTTRTVTHDHAQEFPRYDERLTTRPYRYAYSVAIPDGETAEWALAETR
                     LFKHDLDKGTTAIHDFGPGRHPGEFVFVPRSIDGAEDDGWLIGLVVNMNDETTDLVIL
                     NADDFTGSAQAVVRLPHRVPPGFHGNWVAD"
BASE COUNT          637 a         1185 c         1362 g          743 t
ORIGIN      
        1 ggggcgctcg gttcgggcac cgtaggcggg gtttggggcg tgggcgcagc gaccggcggg
       61 gtcatcgtga tcgtcggcgc ttcctgagcg aacgcggcgg gggtggacag aaccagaaac
      121 gcggcaatcg cgctcgtggc ggggcgggag agggcgatat tcttcatcat agtgaggaag
      181 gaacgagcca cggattgaaa gtgctgccgc taaggcggcg cttccgcgac gagccgtgcg
      241 ccgcgaacgg cgcggcgaag attcttcgat gagggatcgc ggtcggcgaa ccatttttga
      301 ggtgggcgct ggaggtggcg ggccgtcggc gccaggcaag aaaaacccgc tttgcccgga
      361 cactcgataa gagcgtgaca gtgattttgc gctttgatgt cggccgcgtc accaatgcgt
      421 cattcctgcg caaccttggc atgatcaaac gccgcgcatg aagacgaccg atctcgcgcc
      481 cacccggctg cccgaccatc tacgcgaaac gcagcgcctg cttttcatcg ccaaacatgc
      541 gcgctggacg ggcggtctgc atcccgatga cggcaaccat gcgccctatc accgggagat
      601 gcgcgaaacg ctggaggggc ttggcacggc cctgtcgatt gccgacagct atgccgcgct
      661 gttcgaccgg ccggcggtcg acctcgtctt tccgctcctc aaccgcggcg gcttcttcaa
      721 ttccgaaatg ctggttccct tgctgtgcaa ccggcatggc ttgccgtatc tcggcgcttc
      781 gccgatcgtc cgcggcctgt ccgacgacaa gcatctgacc aagctcgagg ccgcggcgcg
      841 cggcgtgccc accgcgccct gggccctttt tcgccgcgga gcgcctgtcg acaccgcgcg
      901 ctgtccgccc gcccggcgct gggtgatcaa gccgaacaac agctcggcat cgtggggcgt
      961 ccgcgatgcg cacgatcgtg ggcaacttgc gcaggcgatc ttcgagattc acgcgcttga
     1021 ccacgacgcg atcgtcgaac cctttatcga tggcagcgat attgaagtgc ccgtggtcac
     1081 gcttaacggc gagcccgcga tgctgccgat gatgatcttc gagcaggcgg acccgacgca
     1141 gctgcgcacc tatcaggaaa agcgcgatct ggtcggtgat gtcggatatg cggtcaagcc
     1201 gttcgatgat ccgttcatcg ccgggcgggt cgccgactat acggcgcgga tggcggacgt
     1261 ctttcgtccg ttcgattacg gccgcttcga attccgcttc gaccatgcga ccggcgaggt
     1321 ccggctgctc gaggtcaatc tcaattgcaa cctgtggtcg gaaaagctgt tttcgcgttc
     1381 cgccatactc gccggcttta cgcacgccga actgatcgag acgatcgtga ccgaaagcat
     1441 gctccgccaa atcgcgcctg cggcggcgcg gcgggatgcg gcgtgagcgg atcgatcctg
     1501 atcctcgccg ggcgtcgggc cggtgcggca gacccactcg cgaataccca tggcgtggcg
     1561 gacaaatgcc tcgtcccggt cgcgggaagg ccgatgatag cgcatgtgct ggacagcgcc
     1621 gccgccagcg cggcgacgca tatattcgtc tcgacgcatc acgccgactt gttgggtgat
     1681 ctcgatgacc cgatcctgaa atcgctgggc gaccggctga tcatcgtgcc ggctgcggac
     1741 aatctggcgg attcggtcct cgcggtcgcc ggccttgcgg aattcccgct tctcgtcacg
     1801 acggccgaca attgcctgct gacgccggcg acgattgccg agatcgaagc cgacgcgata
     1861 cggcggggcg tcgatgcggg cgtggcactg gcgcggcgcg aggatgtgct tgcggtccac
     1921 ccggaaggcc agcggcgctt ttacgcgttt tccgacgtct cggtgtcgaa ctgcaacgcc
     1981 tattggatcg gccatccggg tgccttgaaa gcggcggagg cgtttcgcgg cggcgggcag
     2041 ttcgtcaaga agccgcttcg ggtgatacgg gcgttcggtc tgatcaacct gctgcgcttc
     2101 cgttttggtc tggggccgat ccaccatgtt ttcgcccgta tctcacgtca cctgaaggtc
     2161 gagatcgccc cgctgctgat cagcgacggg gcgacggcga tcgacgtcga caatgaacgg
     2221 tcgttgcggg tgaccgaggc gctgatggcg cgccccgata gaatcaatcc gccacccaat
     2281 tgccatgaaa acccggagga acccggtgcg gcaggcgaac gacggcttgc gctgacccgg
     2341 taaagtcgtc ggcgttcagg atgacgaggt cggtggtctc gtcattcatg ttgacgacca
     2401 ggccgatcag ccagccgtcg tcttcggctc catcgatact ccggggtacg aaaacgaact
     2461 cgccgggatg gcggccgggg ccgaagtcgt ggatcgctgt cgtccccttg tcgaggtcgt
     2521 gtttgaacag gcgcgtttcg gcgagcgccc attcggccgt ctctccatca gggatcgcga
     2581 cgctgtaggc atagcgatag ggccgggtcg tcagccgctc gtcatagcgc gggaattcct
     2641 gcgcatggtc gtgggtgacg gtacgcgtcg tcgtgcgcgc ggcggggtcg atcgtccagc
     2701 gttccatgcg cgatttctgg ctgtcgggcc cgcgcttcga ttcggcgaac atcgtttcgt
     2761 gcgcgacgac gtcgacgatg accttgccat cggcggtttc gaatgcgttg gcgggatgga
     2821 agacatagca gggctcgacc gggacccagg cgatgctgtc gccgctgcca ttcctgggaa
     2881 ggaggccgac ccgggccggg tgcgcatcgt tccatgcgta agggaagcga tagccggcga
     2941 gcagcatctt catcgagaag gtgacgggca ggtcgaagac cagcacgtaa ttttcggtga
     3001 tcgcgcaatc gtggatcgaa gggccgtcgc tcacggcaat cgcttcctcg cggatgacgt
     3061 gcgcatcttt gtcgagcacg acgtgccaga ccttgttcgg ctcgtcgccg cgataggtga
     3121 tcgcgtgcat ctcgcccgag aagggatcgt ggtgcgggtg cgcggtgtag gcgccggcca
     3181 gcgtgccgtc gaacgggtta tgcgcgaacg tgttcaactc gtaaccgagc tcgaccggct
     3241 ttccgcccgc ctcgacgatc gcgaaggtgc ggcccgcgag gccgacgaca ttggtattgg
     3301 gcgcgtcgtt ccggccttcg cgcgggccgg gagggagttc ctcgccaagc atttcgcacg
     3361 cctcgctgcc gcgtacccag cggttgcgat accagtcggc cttgccgccc tcgatgcgga
     3421 tgccgtggac catgccggcg ccggtgaacc agtggtagct cgcgggatcg ggtgcgacgg
     3481 cgggattggg accgttgcgg aggtaacggc ctttgagctg cgccgggatt tcgccgtcga
     3541 cgcgcagttc ctcgagcgtg acctcctcgg tcatcggttt gtggatgccg gtgaggaagg
     3601 ggtgggcgtc ggtggggcgg gggaggcgtt tgcggttgaa gtcggcgacc ttgccgatgc
     3661 ctttggcgac cgcgctgcgg atcagggttt cgacgttgct tgccatggcg atgctcctta
     3721 cggaagttgg cgaaggtgcc gcggagggtt gcgcggacga ctcgatatgt ttacagtggc
     3781 aacattatgg cgagcaaaga taacagtgtc aacttaaaag gcggcggcaa ggccgcgcgc
     3841 gcctatcatc atggcgatct gcgcgcggcg gtgatcgcgg cggggctcga tcggcttgcg
     3901 gagggcgacg gtgccgagct ggggctg
//
LOCUS       DATMSU010000220        11840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_60949, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000220 DATMSU010000000
VERSION     DATMSU010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11840
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60949"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VN034_05615"
     CDS             <1..519
                     /locus_tag="VN034_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWV60109.1"
                     /translation="RIASGRSLYPRLINREFGNEPLPFSPEALAVARHGMDLVVNGAG
                     TAVRSRLPLDGITMAGKTGTAQVRGLRTGNGQSGTWKFRDHGLFVGFAPVDNPRYATG
                     IVIEHGMGGSRAAAPVARDFMTYLFDREKGMEALETLEAGWGGPIKDRMDRDYAAWKS
                     GASSDPDPGVPQ"
     gene            516..1622
                     /gene="rodA"
                     /locus_tag="VN034_05620"
     CDS             516..1622
                     /gene="rodA"
                     /locus_tag="VN034_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWV60110.1"
                     /translation="MIPVPPAIQRIPWKLIAILAGITGFGLLVLYSAAGGNWSPWAWQ
                     QGVRFLVCLGIALVIGRFPIRIFEDFAYIAYIGVLILLIAVELLGFVGGGSQRWLNLG
                     IINLQPSELMKVAIVIALARFYAQLPPGNTRTFTALWPALVMIGLPAALVMLQPDLGT
                     ALAISIGGVVVMFVAGLPFWWFGSTAAAGVAALPILFSFLHDYQQKRVLIFLDPESDP
                     LGAGYHISQSKIAIGSGGIGGKGFLNGSQSHLDYLPEGHTDFIFATMAEEWGLIGGLA
                     LLFGFFLLLRWSTRVALRARTRFGQLTAAGLTMTIFFYIAINLMMVMGLAPVVGIPLP
                     LFSYGGSSMLTIMTCVGIILAIENDSRKGSRRLH"
     gene            1735..1810
                     /locus_tag="VN034_05625"
     tRNA            1735..1810
                     /locus_tag="VN034_05625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1768..1770,aa:Lys,seq:ctt)
     gene            complement(1874..2653)
                     /locus_tag="VN034_05630"
     CDS             complement(1874..2653)
                     /locus_tag="VN034_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWV60111.1"
                     /translation="MTRKRAIIISAEFASFVGHGPVDPAWLPPEPIVIGFGDMGEPAV
                     SILASEDIDHEAGGHILLFAISRTACLRMFGLLPSVKGSWYLSADLRELGRALVAVEG
                     ESEVAGMLRVARSLELLCRLFGALEEHRMVEFHGKTTLSQIDAKRVAAAHQLVSEGWQ
                     EPLTVAEIARRSGLGKAKLTQGFREMYKCTVAEAVSERRLSHARSLLSLSDLPISSIG
                     YRCGYQSNASFTRAFVRRFGVTPTDMRRREKRPHAAAQLSF"
     gene            complement(2752..3039)
                     /locus_tag="VN034_05635"
     CDS             complement(2752..3039)
                     /locus_tag="VN034_05635"
                     /inference="COORDINATES: protein motif:HMM:NF023232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3325 domain-containing protein"
                     /protein_id="HWV60112.1"
                     /translation="MIHLLLFLLAFAGFAMLCAARDRHQRELFGRRLPAKTAVGLRRT
                     GLCLLLLAYPVAGLALGWGYGAVEWLGHLSGGALLTVLLLARRTARGTTGR"
     gene            complement(3036..4604)
                     /locus_tag="VN034_05640"
     CDS             complement(3036..4604)
                     /locus_tag="VN034_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWV60113.1"
                     /translation="MKGGFRQSMAWLHTWTGLLLGWLLFAIFVTGTSAYFQEEITRWM
                     TPEVHSVPADPASGFVAATEWLQREAPGGSEWSIYSTGKRAAGLQLYWANGPDAPADA
                     PTSARLDGTGSEVPARETLGGWFLYRFHYDLHYINWYWARWLVGIAAMAMLVAILSGI
                     VTHKKILADFFLLRFGKGQRSWLDAHNASSVLFLPFILMITYTGLVSLATHYMPWGIA
                     ANYTDEGKFFEETAPWPVPGEKTGPAPLAPIGPMVEHAQHRWGTAAGSVRILNPGDRS
                     AQVIVSSAPDAGMSIALRSLTFDGVTGRPVGAQDPSGAATVTEDTMIGIHAGRFASPL
                     LRALYFLSGLAGCVMVASGLILWTVKRRAKLPDPDRPHFGFRLVEKLNIAAIAGLPFG
                     MAVYFLANRLLPLGIAGRADREIDTMFIAWGAVAIWALARPARRAWLEALSATALAFA
                     LVPVVNALTTDRSLFASLAAGDSVFAAFDGAMLLIAAGFGWAALKLARRPIGQGRKTA
                     SRGSSARVISGETA"
     gene            complement(4601..4909)
                     /locus_tag="VN034_05645"
     CDS             complement(4601..4909)
                     /locus_tag="VN034_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60114.1"
                     /translation="MSKVNRIWRGARWRDIAARALAAVPLNYAVTTALTMLIARLLPG
                     GGAQASIAATMLSFVIFAGLAMTAFAVRSVAKLWIGLVAAGLVAGAADWLLIAMGGRL
                     "
     gene            complement(4960..7131)
                     /locus_tag="VN034_05650"
     CDS             complement(4960..7131)
                     /locus_tag="VN034_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HWV60115.1"
                     /translation="MRKFMACLLLSAALVPAVAVAETADETDASKGNENTIVVTGERF
                     AGEVNSGKTDIPLAELPQAISVISAEQLRERGVTRLADALFSVAGAARSSTYGFYDAY
                     SLRGFDAAYGSVYLDGLLNESGGGGSNNELVGLQSIEVVKGPASSLFGGGPLGGIVNL
                     VSKRPDDKQDFLDVQFSTGSYNLVEGAIDANMRLDRGGAVTARFVGLYRDSDSFVNNA
                     GYNRLYLQPSLSWRPGPDTLLTLIGTLKRDHDNPWAPLNAYGTVFPLAGGYRLPLDFA
                     VGEDGDEKAVQNENRKTISAIFEHGFTDRLKFNATARYMHRTTFWDRWMFAGDFLDEE
                     LDGEGNPIAGTGTTIGRFYYGPYDETFKSFLADSRLSWQVDTGPLRHNLLGGIDYRRT
                     DSRYEGDGDYDQTHFPLNVYDPDYSLPLVPTPAPYSGHDTGRQLGFYVQDHIEIGDRL
                     TLTLNGRWDRAKFNGEPQTAFSPRVGATWEAVPGVSLYANYAKSFTPQFGSQIVLEVG
                     DDGNPSVIGQAPPERGRNIEAGAKFDIADARLAGTIALYQLTRTNVLMTDPQFPLFSI
                     VSGKQRAKGAELELHWRPGAGFGVDLAYTYIRGRYIEDEFSPPGTPLPNIPKHNATLF
                     GHYTVPSGPLAGLGANLGLLYNSNRYTYDSFAYPWQDPMMVLKNYILVNGGLSYAFEG
                     WEARINVNNIFDKRYYPDACCITRVTPGEPRNWRLTISRRF"
     gene            complement(7175..9271)
                     /locus_tag="VN034_05655"
     CDS             complement(7175..9271)
                     /locus_tag="VN034_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HWV60116.1"
                     /translation="MTVRMIALCGAATLAFAANPAWAGEGAEDAAAAAADAGTDEDSI
                     VVTGYAGTKTDTALTELPQPIKVITAEQYEAQGAISISDTVKYAAGVLANPYGRDTRV
                     DGFNVRGLDALQFRDGMRDIFSYYASITSDPYNFSRVEIVRGPASVLFGQGSIGGIVN
                     LVSKTPDFDTHGEINLVYGSFDRKEVLGDVNLALTDNLAVRFVGRARDADTYVDHVPD
                     DRVMFAPSIRWQPTPDTDVVLTGLYQEDDTGSTSQFLPIVGTFRPNTAYPQGKLDRYT
                     FVGKPGEDRYAGRSLQGGGSITHRFSDAVKLSLKARYIDSDLEYNTRYTDSYTNPTDP
                     FLPYAAQGTYGRTIGLYVGANDARMNVFSTDNNVQFNFNTGANIEHKLLVGLDYSWNK
                     VAKRSASGFELIDLYDIDYDAMGTYDPTGPFFVESQKQLGIYVQDQIRFYDRVSVVLG
                     ARRDRVTGSSGQKDNATTFRAGIIGEIGAGFSPFFSYTESFLPVAGSIQDINGDPISP
                     YRPQTGTQYEAGVKWQPNINTLVTATVFKIRERNRVLYGAGNSTSQSGVLNTKGFEIE
                     ASHTLPGNFELLANYGYAKLKSETNTSLDYMPRHTASLWSTKTFGLPDEAQLRLGGGV
                     VYSGKSVSTSPVWSIVTPSRTTVDALAEISWNNWRFAVNATNLLNNKYFASCLARGDC
                     FVGAPRNVMGTVGYRF"
     gene            9429..10064
                     /locus_tag="VN034_05660"
     CDS             9429..10064
                     /locus_tag="VN034_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HWV60117.1"
                     /translation="MKKTFAALMAFGFLASPATAHEVWVERDATGPARIYLGEPADPV
                     PEAGDPEFSKLTAPRLIGADAALVRRANHIEAAVTGNGDVRLTDDNVFAPWEAEGGKW
                     EGVIYYARAGRAEIRSALDLEIVPTAAGANSFAVHWLGKPLPGAKVTVINADRWQKSF
                     AADDQGRIDVTVTGTGRYLLSTSHEVEGARTLGGKSVAKTHHISTLTFRTP"
     gene            complement(10086..11165)
                     /locus_tag="VN034_05665"
     CDS             complement(10086..11165)
                     /locus_tag="VN034_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein B"
                     /protein_id="HWV60118.1"
                     /translation="MTRIALLLSGIAPFVFAVPAAAQSMDHSMHGSQPAAKPAPEAPA
                     CTPEHAAMGHCTLESAPAEKTSDPDMKTMDHGAPASDPACPPEHAAMGHCTPKPAGKT
                     GQAGATGTDLPAGDAPAPPPPGDWYADRYFPASEMDHARHAMMAENGAQTVGFVSLNL
                     AEYQARKGRDGFRWEGEAWYGGDINRLTLKSEGETVFGEGLESGEVQALYSRAIGPYF
                     NAQAGVRQDFGQGPDRTYATIGFEGLAPYWFEVEGALFLSDKGDLLARIEGSYDQRIT
                     QRLLLQPMAELNFAAQDIPESGIGSGLSDVELGLRLRYEIVREFAPYLGVEWARKVGD
                     TARFARADGDDADSVSFVAGVKFWF"
     gene            complement(11162..>11840)
                     /locus_tag="VN034_05670"
     CDS             complement(11162..>11840)
                     /locus_tag="VN034_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWV60119.1"
                     /translation="PAGDASCAPEHAAMGHCTPAASTPDHAAMGHGADAGAGGMEHSM
                     RDFSVAPQVKRDPSVQSISPMPVDRMGEPGQGLEDVGHKVLTYHDLVALDPNPDTRAP
                     SRSLDIHLTGNMERFMWSFDGVKMSDHHEPIPFIEGERVRVNLINDSMMSHPIHLHGH
                     FFELVTGKGDRAPRKHTVIVQPGGIATFDFTADALGDWAFHCHLLYHMHAGMMRVVSV
                     RPRGDGE"
BASE COUNT         2051 a         3766 c         3937 g         2086 t
ORIGIN      
        1 cggatcgcat cggggcgcag cctctatcca cgcctcatca accgcgagtt cgggaacgag
       61 ccactgccct tctcgcccga agcgcttgcc gtcgcgcgcc acggcatgga cctcgtcgtc
      121 aatggcgcgg gcaccgccgt gcgcagccgc ctgccgctcg acggcatcac catggcgggc
      181 aagacgggca ccgcgcaggt gcgcgggctc cgcaccggca acggtcaaag cggaacgtgg
      241 aaatttcgcg atcacgggct gttcgtcggc ttcgcgccgg tcgacaatcc gcgctacgcc
      301 accgggatcg tcatcgagca tggcatgggc ggcagccgcg cggcggcgcc cgtcgcccgg
      361 gatttcatga cctatctgtt cgaccgcgaa aagggaatgg aagcgctgga gacgctcgag
      421 gccggctggg gcgggccgat caaggatcgc atggaccgcg actatgccgc atggaaatcg
      481 ggcgcgtcga gcgatcccga ccccggagtg ccgcaatgat ccccgttccg cccgcgatcc
      541 agcgcatccc gtggaaactg atcgccatcc tcgccgggat caccggcttt ggcctgctcg
      601 tcctctattc ggctgcgggc ggcaattggt cgccctgggc gtggcagcag ggcgtgcgct
      661 ttctcgtctg cctcggcatc gcgctggtga tcggacgctt cccgatccgc attttcgagg
      721 atttcgccta tatcgcctat atcggcgtgc tgatcctgct catcgcggtc gagctgttgg
      781 gcttcgtcgg cgggggcagc cagcgatggc tgaaccttgg catcatcaac ctccagccgt
      841 ccgaactgat gaaggtcgcg atcgttatcg ccctcgcccg tttctacgcc cagttgccgc
      901 ccggaaacac gcgcaccttt accgcgctct ggcccgcgct ggtgatgatc gggctgcccg
      961 ccgcgctcgt catgctgcaa cccgatctcg gcaccgcgct cgccatctcg atcggcgggg
     1021 tggtggtgat gttcgtcgcg ggcctcccct tctggtggtt cggcagcacc gccgccgcgg
     1081 gcgtcgccgc gctgcccatc ctcttctcct tcctgcacga ttatcagcag aagcgcgtgc
     1141 tgatcttcct cgaccccgaa agcgatccct tgggcgccgg ctatcacatc agccagtcga
     1201 agatcgcgat cgggtcgggc ggcatcggcg gcaagggctt cctcaacgga tcgcaaagcc
     1261 acctcgacta tctgcccgag gggcacaccg acttcatttt cgccaccatg gcggaggaat
     1321 ggggactgat cggcgggctg gcgctcctgt tcggcttttt cctgctgctg cgctggtcga
     1381 cgcgcgtcgc gctgagggcg cggacgcgct ttggccagct caccgccgcc ggcctcacaa
     1441 tgacgatctt cttctatatc gcgatcaacc tgatgatggt gatggggctc gcgcccgtcg
     1501 tcggcattcc gttgccgctc ttttcctacg gcggctcgtc gatgctgacg atcatgacgt
     1561 gcgtcggcat catcctcgcg atcgaaaacg acagcaggaa gggcagccga cgcctgcatt
     1621 gataaaaaaa ttcggcaagg ccattgccaa gggtccagca ccatgatagc gggccgctct
     1681 cttcacgacg cagagggatt ttccttggtt tcgtgaacgc cggaaaccgg cagtggacgc
     1741 atagctcagt tggtagagca gctgactctt aatcagcggg tcctaggttc gagccctagt
     1801 gcgtccacca tttctttcaa agacttagcc tgcatttccg ccgtctcgcg gcgacgcgaa
     1861 tatggcatgc gcgctagaat gaaagttggg cggccgcgtg gggccgcttt tcgcgacgac
     1921 gcatgtcggt cggcgtcacg ccgaaccggc gcacgaaggc gcgcgtgaag ctggcgttgc
     1981 tttgatagcc acagcgatag ccgatgctgg aaatcggcag gtcgctcagc gacagcaagc
     2041 tgcgcgcatg cgacagccgc cgctcgctca ccgcctccgc gacggtgcat ttatacattt
     2101 cgcgaaagcc ctgcgtcagc ttggccttgc cgagccccga gcgtcgcgcg atctccgcca
     2161 cggtcaatgg ctcctgccag ccttcgctga ccagctggtg cgcggcggcg acgcgcttgg
     2221 cgtcgatctg gctgagtgtc gtcttgccgt ggaattcgac catccgatgc tcttcgagcg
     2281 cgccgaacag ccggcagagc agttcgaggc tgcgcgcgac gcggagcatg cccgccactt
     2341 cgctttcgcc ttcgaccgcg accagcgcgc gaccaagctc gcgcaggtcg gcggagagat
     2401 accagcttcc cttcacgctc ggcagcaggc cgaacatgcg caggcacgcg gtgcggctga
     2461 tcgcgaacag caggatatgg ccgccggctt cgtgatcgat atcctcgctc gccaatatgc
     2521 tgaccgcggg ttcgcccata tcgccgaagc cgatgacgat cggctcgggc ggcagccacg
     2581 ccggatcgac gggcccgtgt ccgacgaaac tggcgaattc cgccgatatg atgatggcgc
     2641 gcttgcgtgt catcgaacct gccagcctct cccgaacagc gtggatcagg ctcccttaga
     2701 atcccgcgag caatgcgtcc agttcggccg gcggcgcctt tgacggcggg atcagcggcc
     2761 ggtggttccg cgcgccgtcc ggcgcgcgag aagcaggacg gtgagcagcg cgccgccgct
     2821 gagatggccg agccattcga ccgcgccata gccccatccg agcgcgaggc cggcgaccgg
     2881 ataagcgagc agcaggaggc acaggccggt acggcgcaag ccgacggcgg ttttcgcggg
     2941 aagcctgcgg ccgaaaagct cgcgctgatg ccggtcgcgc gcggcgcaaa gcatcgcgaa
     3001 acccgcgaag gcgagcagga agagcaggag gtggatcatg ccgtctcacc ggatattacg
     3061 cgcgccgacg atccgcgcga tgcggtcttg cgaccctgtc cgatcggcct gcgcgcgagc
     3121 ttgagcgccg cccagccaaa gccggcggca atgaggagca tcgcgccgtc gaatgcggca
     3181 aagacgctgt cgccggcggc aaggctggcg aagagcgacc ggtcggtggt caaggcgttg
     3241 acgacgggca ccaatgcaaa ggcgagcgcg gtcgccgaca aggcttcgag ccatgcgcgt
     3301 cgggcaggcc gcgccagcgc ccatatcgcc acggcacccc aggcgatgaa catggtatcg
     3361 atctcgcggt cggcgcggcc ggcgataccg agcggcagca ggcggttggc gaggaagtag
     3421 accgccatcc cgaagggcag gcccgcgatc gccgcgatgt tcagcttttc gacgagccgg
     3481 aagccgaaat gcggccggtc ggggtctgga agcttggcgc ggcgcttgac cgtccacagg
     3541 atcaggcccg aagcgaccat gacgcagccc gccagtcccg acagaaaata gagcgcgcgc
     3601 agcagcggcg aggcgaagcg gccggcgtgg ataccgatca tcgtgtcttc ggtcaccgtc
     3661 gcggcgcccg acgggtcttg cgcgccgacc ggcctgcccg tcaccccgtc gaaggtcagc
     3721 gagcgcaggg cgatcgacat gcccgcatcg ggcgcgctcg acacgatgac ctgcgccgaa
     3781 cggtcgccgg ggttgaggat gcgcacgctg cccgccgccg ttccccagcg atgctgggca
     3841 tgctcgacca tcggcccgat gggagcaagc ggggccggcc cggtcttttc tccgggcacg
     3901 ggccagggcg cggtctcctc gaaaaatttg ccctcatcgg tgtagttggc ggcgattccc
     3961 cagggcatat agtgcgtcgc caggctgacg aggccggtgt aggtgatcat caggatgaag
     4021 ggcaggaaca gcacgctcga cgcattatgc gcgtcgagcc agctccgctg ccccttgccg
     4081 aagcggagca ggaagaaatc cgccagaatc ttcttgtgcg tgacgatacc gctgaggatc
     4141 gcgacgagca tcgccatcgc cgcgatgccg accagccagc gcgcccaata ccagttgatg
     4201 taatgaagat cgtaatgaaa gcggtatagg aaccagccgc ccagagtctc gcgcgccggg
     4261 acctcgctgc cggtaccgtc gaggcgcgcg ctggtaggcg cgtcggcggg ggcgtcgggt
     4321 ccgttcgccc aatagagttg caggccggcg gcgcgcttcc ccgtcgaata gatcgaccat
     4381 tcgctgccgc cgggcgcctc gcgctgcaac cattcggtcg ccgcgacaaa gccgctcgcc
     4441 ggatcggcgg ggacgctgtg cacctcgggc gtcatccagc gcgtgatctc ttcctgaaaa
     4501 taggccgaag tcccggtcac gaagatggcg aacagcagcc agccgagcaa cagcccggtc
     4561 caggtgtgca gccacgccat cgattgccgg aagccaccct tcacagccgg cctcccatgg
     4621 cgatcagcag ccagtcggcc gcccccgcga ccagcccggc ggcgaccagc ccgatccaca
     4681 gcttcgcgac cgagcggacc gcgaaggcgg tcatggcgag gcctgcgaag atcacgaaag
     4741 agagcatcgt cgcggcgatc gaggcctgcg ccccgccgcc cggcaacagc cgcgcgatca
     4801 gcatggtcag cgcggtcgtg accgcatagt tgagcggcac cgcggccagc gcacgcgccg
     4861 cgatgtcgcg ccaccttgcg ccgcgccaaa tccggttcac cttgctcatg gcgcccctat
     4921 cgcaacattg cgactgactc gcaatagcct gctccatcat cagaagcggc ggctgatcgt
     4981 caggcgccag tttcgcggct cgccgggcgt cacgcgcgtg atgcagcagg cgtcgggata
     5041 gtagcgcttg tcgaagatgt tgttgacgtt gatccgcgct tcccaccctt cgaaagcata
     5101 ggagagcccg ccgttgacga ggatgtagtt tttgaggacc atcatcgggt cctgccaggg
     5161 ataggcgaag ctgtcgtatg tgtagcgatt gctgttgtag agcagcccca agttcgcgcc
     5221 gagcccggcg agcggtcccg acggcaccgt ataatgaccg aacaacgtcg cattatgttt
     5281 cgggatattg ggcagcggcg ttccgggcgg cgaaaactca tcctcgatgt agcggccacg
     5341 gatataggtg taggcgagat cgacgccgaa gccggcgccg gggcgccagt gcagttcaag
     5401 ctcggcgccc ttggcgcgct gcttgcccga gacgatcgag aacagcggga actgcgggtc
     5461 ggtcatcaac acattggttc gcgtcagctg atagagcgcg atcgtcccgg cgaggcgtgc
     5521 atcggcgatg tcgaatttgg caccagcctc gatattgcgg ccgcgctcgg gcggagcctg
     5581 tccgatgacc gacgggttgc cgtcgtcgcc gacctcgagc acgatctgcg agccgaattg
     5641 cggcgtgaag gatttggcgt aattggcata gagcgacacg cccggcaccg cctcccaggt
     5701 cgcgccgacg cgcggcgaaa aggcggtttg cggttcgccg ttgaacttcg ccctgtccca
     5761 gcggccgttg agcgtcagcg tcaggcggtc gccgatttcg atatggtcct ggacgtagaa
     5821 gccaagctgg cggccggtgt catgccccga ataaggtgcc ggcgtcggga cgagtggcag
     5881 cgaatagtcg ggatcataga cgttgagcgg gaaatgcgtc tggtcgtaat cgccgtcgcc
     5941 ctcatagcgc gaatcggtcc ggcgatagtc gatgccgccg agcagattgt ggcggagcgg
     6001 cccggtgtcg acctgccagc tcagccggct gtcggcgagg aaggatttga aggtctcgtc
     6061 gtacggacca taatagaagc gtccgatcgt cgtcccggtg cccgcgatcg gattgccctc
     6121 gccgtcgagt tcctcgtcga gaaaatcacc cgcgaacatc cagcggtccc aaaaggtcgt
     6181 gcggtgcatg taacgcgcag tcgcattgaa cttcagccgg tcggtgaagc cgtgctcgaa
     6241 gatagcgctg atcgtcttgc gattctcgtt ctgcaccgcc ttctcgtcgc catcctcgcc
     6301 gaccgcgaaa tcgagcggga ggcgatagcc gcctgcgagc gggaagacgg tgccataggc
     6361 gttgagcggc gcccaagggt tgtcgtgatc acgcttcaac gtgccgatca gggtcagcag
     6421 cgtgtcgggg ccgggccgcc agctcagcga cggctggaga tagaggcggt tgtagccggc
     6481 attgttcacg aagctgtcgg aatcgcgata gaggcctacg aagcgcgcgg tgaccgcacc
     6541 gccccgatcg agccgcatgt tggcgtcgat cgcgccctcg acgagattgt aggatccggt
     6601 cgagaattgg acgtcgagaa aatcctgttt gtcgtcgggc cgcttgctca ccagattgac
     6661 aatgccgccg agcgggccgc cgccgaacag cgacgacgcc gggcctttga ccacctcgat
     6721 cgattgcagc cctacgagtt cgttgttgct gccgcccccg ccggactcgt tcagcaatcc
     6781 gtcgaggtag acgctgccat aggcggcgtc gaagccgcgg agcgaatagg cgtcgtaaaa
     6841 gccataggtg ctgctgcgcg ccgcgccggc gaccgagaaa agcgcgtcgg cgaggcgggt
     6901 gacgccgcgt tcgcggagtt gctcggcgct gatgaccgaa atcgcctgcg gcagttcggc
     6961 aagcgggatg tcggtcttgc ccgaattcac ctcgccggcg aaccgctcgc cggtcacgac
     7021 gatcgtgttt tcattgccct tcgaagcgtc cgtctcgtca gccgtttcgg cgacggcgac
     7081 cgcgggtacg agcgccgccg aaagcagcag acaagccatg aatttccgca tgatgaaccc
     7141 cttcccctgc ggccggaaca gcgattgcca gcgatcagaa gcggtagccg accgttccca
     7201 tcacattgcg cggtgcgccg acgaaacagt cgccgcgcgc aaggcaggag gcgaaatatt
     7261 tgttgttgag caggttcgtg gcgttgaccg cgaagcgcca gttgttccag ctgatctcgg
     7321 cgagcgcatc gaccgtcgtg cgcgacggcg tgacgatcga ccagaccggg ctggtcgaca
     7381 cgcttttgcc gctatagacg acgccgccgc cgaggcgcag ctgcgcttcg tcgggaaggc
     7441 cgaaggtctt cgtcgaccag agcgatgccg tgtggcgcgg catatagtcg aggctggtgt
     7501 tggtttccga cttcagcttc gcatagccat agttggcgag aagttcgaaa ttgcccggca
     7561 gcgtgtggct cgcctcgatt tcgaagccct tggtgttgag gacgcccgac tggctggtcg
     7621 agttgccggc gccatagagg acgcgattgc gctccctgat tttgaagacg gtggcggtga
     7681 ccagcgtgtt gatgttcggc tgccacttca cgccggcttc atattgcgtg ccggtctgcg
     7741 gccggtaggg cgagatggga tcgccattga tgtcctgaat cgagcccgcg accggcagga
     7801 agctttcggt atagctgaag aagggcgaga agcccgcgcc gatctctccg atgatcccgg
     7861 cgcggaaggt ggtggcattg tccttctgac cggaggagcc ggtgacgcgg tctcgacggg
     7921 cgcccagcac gacggatacc cggtcgtaga agcggatctg gtcctgcaca tagatgccaa
     7981 gctgcttctg gctttcgacg aagaatgggc cggtcggatc gtatgtaccc atcgcgtcat
     8041 aatcgatgtc gtagagatcg atcagttcga acccgctggc gctgcgcttg gcaaccttgt
     8101 tccagctata gtcgaggcca accagcagct tatgttcgat atttgcgccg gtgttgaagt
     8161 tgaactggac gttgttgtcg gtcgaaaaga cgttcatccg cgcgtcgttg gcgccgacat
     8221 acaggccgat ggtgcggcca taggtgcctt gggcagcata gggcaggaac gggtcggtcg
     8281 ggttggtata gctgtcggtg tagcgggtgt tatattcaag gtcgctgtcg atatagcgcg
     8341 ccttcaggct gagcttcaca gcgtccgaga agcgatgcgt gatcgagccg ccgccttgga
     8401 gcgatcggcc cgcatagcgg tcctctccgg gcttgccgac gaaggtgtag cggtcgagct
     8461 tgccctgcgg ataggcggtg ttgggccgga aggtgccgac gatcggcagg aattgcgagg
     8521 tcgagccggt atcgtcctcc tgatagaggc cggtcagcac gacgtcggtg tcgggcgtcg
     8581 gttgccagcg gatcgagggc gcgaacatga cgcggtcgtc ggggacatgg tcgacatagg
     8641 tgtcggcatc gcgcgcgcgg ccgacgaagc ggaccgcgag attgtcggtg agcgcgaggt
     8701 tgacgtcgcc cagcacttcc ttgcggtcga agctgccata gacgaggttg atttcgccgt
     8761 gcgtgtcgaa atcgggcgtc ttgctgacga ggttgacgat gccgccgatc gaaccctgcc
     8821 cgaacagcac cgaggccgga ccgcgcacga tctcgacgcg cgagaaatta tacgggtccg
     8881 acgtgatcga ggcatagtag ctgaagatgt cacgcatgcc gtcgcggaac tgcagcgcgt
     8941 cgagcccgcg gacgttgaag ccatcgacgc gggtgtcgcg gccataggga ttggcgagga
     9001 cgccggcggc atatttcacc gtgtcgctga tgctgatcgc gccctgcgcc tcatattgtt
     9061 cggcggtgat gaccttgatc ggctgcggca attcggtcag cgcggtgtcg gtcttggttc
     9121 cggcatatcc ggtgacgacg atgctgtcct cgtccgtacc cgcatcggca gcggcggcgg
     9181 cggcatcctc cgccccctcg cctgcccagg ccggattggc agcaaaagcc agcgtggctg
     9241 ctccgcagag cgcgatcatt cggacggtca tggtgcttcc ctttttgcga ctcaatctca
     9301 attaggggcg gcagctagag cgaacgtgtc gcaatagcaa gctagtaata atcgtttgca
     9361 ataggaagct cctgccgcca cacagcggtc agctaacgac acggacattc ggacagaggg
     9421 aggtccccat gaaaaagact tttgccgcgc tgatggcctt cggcttcctt gcgtcacccg
     9481 cgacggccca cgaggtttgg gtcgaacgtg acgccacagg cccggcgcgc atctatctgg
     9541 gcgaacccgc cgatccggtt cccgaagccg gcgatcccga attttcgaag ctgaccgcgc
     9601 cgcgcctgat cggagccgac gcggcgctgg tccgccgcgc gaaccatatc gaagccgcgg
     9661 tgaccggcaa cggcgacgtc cgcctgaccg acgacaatgt gttcgcgccg tgggaggccg
     9721 aaggcggcaa atgggagggc gtgatctatt acgcccgcgc cggccgcgct gaaatccggt
     9781 cggcgctcga tctcgaaatc gttccgaccg ctgccggagc aaacagcttc gccgtccatt
     9841 ggctcggcaa gccgctgcct ggcgcgaagg tgacggtgat caacgccgac cgctggcaaa
     9901 aaagcttcgc cgccgatgat cagggccgca tcgacgtcac ggtgacgggc accggccgtt
     9961 atctgctatc gacctcgcac gaggtcgagg gtgcccgcac gctcggcggc aagagcgtcg
    10021 cgaagaccca ccatatctcg acgctgactt tccgcacgcc atagcgttgc gggaaagtca
    10081 gcgccctaga accagaattt caccccggcg acgaagctga cgctgtcggc atcgtcgccg
    10141 tcggcgcgcg cgaagcgcgc ggtgtcgccg actttccgtg cccattcgac gccgagatag
    10201 ggcgcgaatt cgcggacgat ctcgtagcgt agtctgaggc cgagttcgac atcggacaag
    10261 cccgagccga tcccgctttc ggggatgtcc tgcgcggcga aattgagttc ggccatcggc
    10321 tgaaggagca gccgctgcgt gatgcgctgg tcgtaactgc cctcgatccg ggcgagaagg
    10381 tcgcccttgt ccgacaggaa cagcgccccc tcgacctcga accagtaagg cgcgagcccc
    10441 tcaaagccga ttgtcgcata ggtgcggtcg gggccctgcc cgaagtcctg acgaacaccc
    10501 gcctgcgcgt tgaaataggg gccgatcgcc cggctgtaga gcgcctgtac ctcgccgctt
    10561 tcgaggcctt cgccgaacac cgtctcgcct tcgctcttca gggtcagccg gtttatgtcg
    10621 ccaccgtacc acgcctcgcc ttcccatcgg aaaccgtcgc gccccttgcg cgcctgatat
    10681 tcggcgaggt tgaggctgac gaagcccacg gtctgcgcgc cattttcggc catcatcgca
    10741 tggcgcgcat gatccatctc gctcgcgggg aaatagcggt cggcatacca gtcgccgggg
    10801 ggcggcggtg cgggcgcgtc gcctgcgggc aggtcggtgc cggtagcacc cgcttgaccg
    10861 gtcttcccgg ccggctttgg cgtgcaatgc cccatcgccg catgttcggg cgggcaggcg
    10921 ggatcggacg ccggcgcgcc atgatccatc gtcttcatgt ccgggtctga tgttttttcg
    10981 gccggcgcgc tttcgagcgt gcaatgcccc atggcggcat gctcgggcgt gcaggccggc
    11041 gcttcgggtg cgggtttcgc cgcgggctgc gagccgtgca tcgaatgatc catcgactgc
    11101 gcggcggccg gcaccgcgaa aacgaacggc gcgatgcccg agagaaggag cgcgatccgg
    11161 gtcattcgcc gtctccccgc ggacggacgc tgacgacgcg catcatcccc gcgtgcatgt
    11221 gatagagaag gtgacagtga aaggcccagt cgccgagcgc gtcggcggtg aagtcgaagg
    11281 tcgcgattcc gcccggctgg acgatcacgg tgtgcttgcg cggtgcccga tcgcccttcc
    11341 ccgtcaccag ttcgaagaaa tggccatgca ggtggatcgg gtggctcatc atcgaatcgt
    11401 tgatcaggtt cacgcgcacc cgctcgcctt cgatgaaggg gatgggctcg tgatggtcgg
    11461 acatcttcac cccgtcgaac gaccacatga agcgttccat attgccggtg aggtggatgt
    11521 cgagcgaacg gctgggcgcg cgggtgtcgg gattgggatc gagcgcgacg aggtcgtgat
    11581 aggtgagcac cttgtgcccg acatcttcca gcccctgccc cggctcgccc atccggtcga
    11641 ccggcatggg ggagatcgac tggacgctcg ggtcgcgctt gacctgcggc gccaccgaga
    11701 agtcgcgcat gctatgctcc ataccgcccg cgcccgcgtc ggcaccatga cccatggcgg
    11761 catgatcggg cgtgctcgcg gccggcgtgc aatgccccat cgcggcatgt tcgggcgcac
    11821 aggacgcatc gccggcgggc
//
LOCUS       DATMSU010000221         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_32345, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000221 DATMSU010000000
VERSION     DATMSU010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32345"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VN034_05675"
     CDS             <1..256
                     /locus_tag="VN034_05675"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWV60120.1"
                     /translation="RVHSGATASPWRAQLGAFGVEANARNLWNSLEKKYPAIAQRQPY
                     LVKGGKLTRLQAGGFANKAEAESFCATVRKGGQACLVVDK"
     gene            complement(341..1210)
                     /gene="bla"
                     /locus_tag="VN034_05680"
     CDS             complement(341..1210)
                     /gene="bla"
                     /locus_tag="VN034_05680"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HWV60121.1"
                     /translation="MIEAISMLAWLAGVSGAPVPALDPLTQPIETSRSAEWLAPAEPK
                     KLYGNTYLVGFGGMSVALIDTGDGLVLVDGALPQAAPAILANVRKLGFDPADIKFILS
                     TEPHFDHAGGIAALARDTGATVVASARGAEGLRAGKHAADDPQLAYGGSWPAVAEVRA
                     VKDGEVLVPGKAAITAHATPGHTMGSMSWSWRACEGRDCKAIVFASSLNPVSSDGYRY
                     TASASAPIVAGFRASYHTMDRLACDILISAHPDNAGSGRFSAAPGACRAYADRSRKLL
                     AKRLAEEKAGTAK"
     gene            1306..1965
                     /locus_tag="VN034_05685"
                     /pseudo
     CDS             1306..1965
                     /locus_tag="VN034_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053939.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          334 a          765 c          626 g          328 t
ORIGIN      
        1 tcgagtccac agcggggcga ccgcaagccc ctggcgcgcc cagctcggcg ccttcggggt
       61 cgaggcgaac gcgcgcaatc tctggaattc gctcgaaaag aaatatccgg ccatcgctca
      121 gcgccagccc tacctggtca agggcggcaa gctcacgcgc ctgcaggccg gcggcttcgc
      181 aaacaaggct gaagccgaaa gcttctgcgc gacggtgcgc aaaggcggcc aggcctgtct
      241 ggtggtcgac aaatagggtg tagccgctac accccggcga aagccgggac ccggccggac
      301 tgcgccgcaa cgggccccgg ctttcgccgg gaagcacata ctacttcgcc gtccccgcct
      361 tctcctccgc caaccgcttc gccagcaatt tgcgcgaccg gtcggcatag gcacggcacg
      421 cgccgggcgc ggcgctgaag cgcccgcttc ccgcattgtc ggggtgcgcc gaaatcagga
      481 tgtcacaagc cagcctgtcc atcgtgtgat aactcgccct gaaccccgcg acgatcggcg
      541 cgctggcgga cgccgtgtag cgatagccat cggacgacac cggattgagg ctcgacgcaa
      601 agacgattgc cttgcagtcg cgtccctcgc acgcgcgcca gctccagctc atgctgccca
      661 tcgtgtgccc cggcgtcgca tgcgcggtga tcgcagcctt gccgggcacc agcacctcgc
      721 cgtccttcac ggcgcgcact tcggcgactg cgggccagct gccgccatag gcgagctgcg
      781 gatcgtcagc ggcatgcttc cccgcacgca gcccctcggc accgcgcgcg ctcgcgacca
      841 cggtcgctcc ggtgtcgcgc gccagcgcgg cgatcccgcc ggcatggtcg aaatgcggct
      901 cggtgctcag aataaatttg atgtctgcag gatcgaaccc gagcttgcgg acattggcaa
      961 ggatcgccgg cgccgcctgc ggcaacgccc cgtcgacgag caccagccca tcacccgtat
     1021 cgatcaaggc aacgctcatc ccgccgaagc cgacgagata ggtattgcca tagagtttct
     1081 tcggttctgc gggcgccagc cattcggccg atcgcgaggt ttcgatcggc tgcgtcagcg
     1141 ggtcgagcgc cgggaccggt gccccgctta cccccgcgag ccatgccaac atgctgattg
     1201 cttcgatcat tttcgtctcc cttcggcagt cgaatgccaa tcgatcgcgc agcccacaaa
     1261 gcatcatttc tcgacaaagc catgagaata attgatggct tggtcatgga ccgcgcccaa
     1321 cttccgctca acgccctgcg cgccttcgag gcggcggcac gccacctcaa tttcacgcgc
     1381 gccgccatcg aacttcacgt cagccagggt gcggtcagcc atcaggtcgc gcaactcgag
     1441 cgccgcctcg gcacgcgcct cttccaccgc ctgccgcgcg ggctcgcgct caccgacgag
     1501 gggcatgcgc ttgttcccgt cgtcgccgac gccttcgacc gcgtcgccgc gacgctcgac
     1561 caatatgcgg gcggccgctt tcgcgaggcg ctcaaggtcg gcgtggtcgg caccttcgcg
     1621 accggctggc tgctcgcgcg cctcgatgcg ttcgcccgcg cgcatcccgc gatcgacctc
     1681 cgcatctcga ccaacaacaa ccgcgtcgat ctcgcgggcg aggcgctcga ctatgcgatc
     1741 cgcttcggcg acggcgcctg gcacggcacc cacgccgagc ctctatgcgc cgcgccgatg
     1801 agcccgctct gcacccccga catcgccgcg cccctcaata cgcccgccga cctcgtctcc
     1861 gaacgtctgc tccgctcgta ccgcgccgac gaatgggcgc tgtggttcga cgccgcgggc
     1921 gtcgcggtgc ccgtgctgcg cggcccgatg ttcgacagct cggcgagaac atgagcggta
     1981 acggcgacat cctcggcttc ggccacaact gcgagcagac catcaacggc atcggtttcg
     2041 gcgcgcattc ggc
//
LOCUS       DATMSU010000222         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_89441, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000222 DATMSU010000000
VERSION     DATMSU010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89441"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..1563)
                     /locus_tag="VN034_05690"
     CDS             complement(349..1563)
                     /locus_tag="VN034_05690"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWV60122.1"
                     /translation="MSTFKPHVSAALNRIQPSATLAMTARVTALKASGVDVIGLSAGE
                     PDFDTPDFVKEAAIEAIRNGQTKYTLVDGTVALKDAIRGKFRRDNGLDFGLDQISVNV
                     GGKHTLFNALVATVDPGDEVIIPAPYWVSYPDIVAFAGGTPVIVEGTAAQNYKITPAQ
                     LEAAITKKTRWVMFNSPSNPSGAAYSGEELDALGEVIRRHPHVMVMTDDMYEHVWYAD
                     FEFSTFAQRCPDLIDRILTVNGCSKAYAMTGWRIGYAGGPAWIIKAMGKLQSQSTSNP
                     CSIAQAAAAAALGGPQQFLDDRNAAFRKRRDMVVAMLNDAPGLDCPVPDGAFYVYPDA
                     SGCIGKKTPDGKLIDSDEALIDYFLDSAKVAAVHGGAFGLSPAFRVSYATSEAVLKEA
                     CVRIQQACAALS"
     gene            complement(1690..>2082)
                     /locus_tag="VN034_05695"
     CDS             complement(1690..>2082)
                     /locus_tag="VN034_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HWV60123.1"
                     /translation="LAELPVPVAVRSAPMAADDFRLAHKTSLRADYDSARHESGAAEV
                     VFVDEPGFVTEGSWSNIFVEREGQLLTPPLALGLLPGVLRAELIDKGRAVESHLRLTD
                     LESGFFIGNSLRGLVPARLAEAAASAIG"
BASE COUNT          390 a          667 c          675 g          350 t
ORIGIN      
        1 gcccgccgca gcggcgtcat gccgggatcg gcaacgccgg ctgattgagg acgatgtaag
       61 tcgcgccaat cgcgaagccg gcgagcatgg tcaaaaacca atcggagcgg agcatggaga
      121 gcataggggc ctcttatcat attatcttgt tcgcggccgg agccgccctg gttacaccgg
      181 cgatatgacg cgcccctgtc atccgtcagt gatggccgtc actctaaaca agatgactta
      241 ccatcgggtg aaccgggcgc ttaaccagcg ttcaggttca aatagtccgg cccgccgaca
      301 ttacaagacc gatcggtata aaattcctat gcggcagtaa accgacgctt agctcagcgc
      361 cgcgcacgcc tgctgaatac gcacgcacgc ttccttcagc accgcttcgg aggtcgcata
      421 ggatacgcgg aacgccggcg acaggccgaa ggccccgcca tgcaccgccg cgaccttcgc
      481 cgagtcgagg aaatagtcga tcagcgcttc gtcgctatcg atcagtttgc cgtcgggcgt
      541 cttcttgccg atgcagcccg acgcgtcggg atagacatag aaagcgccgt cggggaccgg
      601 gcaatcgaga cccggcgcgt cgttgagcat cgcgaccacc atgtcgcggc gcttcctgaa
      661 cgcggcgttg cggtcgtcga gaaactgctg cggcccgccg agcgcggcgg cggcggcggc
      721 ctgcgcgatc gagcaggggt tcgaggtcga ctgcgactga agcttgccca tcgccttgat
      781 gatccacgcg ggcccgcccg catagccgat gcgccagccg gtcatcgcat aggccttgga
      841 gcagccattg accgtcagga tacggtcgat caggtcgggg cagcgctgcg cgaaggtcga
      901 gaattcgaaa tcggcgtacc agacgtgctc gtacatatcg tcggtcatca ccatgacgtg
      961 cggatggcgg cggatgacct cgccgagcgc atcgagttcc tcgcccgaat aggcggcgcc
     1021 cgacgggttc gacggcgagt tgaacatcac ccagcgcgtc tttttggtga tcgccgcctc
     1081 gagctgcgca ggggtgatct tgtaattctg cgccgcggtg ccctcgacga tcaccggcgt
     1141 gccgcccgca aaggcgacga tgtcgggata gctgacccaa tagggcgcgg ggatgatcac
     1201 ctcgtcgccc ggatcgacgg tcgcgacgag cgcgttgaac aaagtatgct tgccgccgac
     1261 gttgaccgaa atctggtcga gcccgaaatc gagaccattg tcgcggcgga acttgccgcg
     1321 gatcgcgtcc ttcagcgcca ccgtgccgtc gacgagcgta tatttggtct gcccgttccg
     1381 gatcgcctcg atcgccgctt ccttgacgaa atcgggggtg tcgaaatcgg gttcgcccgc
     1441 gctgaggccg atgacatcga cgcccgacgc tttcagcgcc gtcacgcgcg cggtcatcgc
     1501 gagcgtcgcc gacggctgga tgcggttgag ggcggcggag acatggggct tgaaagtcga
     1561 catcgaaaag cgtccttgcg agagggcgaa cggcgtgaac tggcagaaaa ttgcgcgcgg
     1621 ccgcgcctaa agcctccggc cgacattcgc aagcgcgcag cttgataatt tacctatgcg
     1681 gcgggcaggt cagcctatcg cggatgccgc agcctccgcg agccgcgccg ggaccaagcc
     1741 gcgcaagctg ttgccgatga agaagccgct ttcgagatcg gtaagacgca ggtgcgattc
     1801 gaccgcgcgc cccttgtcga tcagctcggc ccgcagcacg ccgggaagca gccccagcgc
     1861 cagcggcggc gtgagcaact ggccctcgcg ctcgacgaag atattgctcc agctgccttc
     1921 ggtgacgaac cccggttcat cgacgaaaac gacctcggcc gcgccgctct cgtgccgcgc
     1981 gctatcatag tcggcgcgca ggctggtctt gtgcgcgagg cgaaagtcgt cagccgccat
     2041 cggcgccgag cgcaccgcga ccggcaccgg caattcggcg ag
//
LOCUS       DATMSU010000223         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_70117, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000223 DATMSU010000000
VERSION     DATMSU010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70117"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VN034_05700"
     CDS             complement(<1..290)
                     /locus_tag="VN034_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV60124.1"
                     /translation="MRLLVAALDDAVEQAYRDAGLNFRPRFYPYFRLLMARESASVGE
                     CVAALGFTQPAATQTLQTMVREGLIELVPGRDRRERRFALTITARSMIPDLEA"
     gene            complement(418..2583)
                     /locus_tag="VN034_05705"
     CDS             complement(418..2583)
                     /locus_tag="VN034_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWV60125.1"
                     /translation="MFLAAIATPLVASPSLFAPQLFAREKDPALTSPTPALSAAPVAE
                     KRPHETTLHGKTLSDPYHWLKDESYPVIDDKDVLDYVKAENAWFDAAMKPHAQLVETL
                     FQEMKGRIKEADSSVPQKDGDWVYWVEYEEGAEYKKWYRKPASGVGDTQLILDEVAMA
                     AGKDYFRLAELSVSPNGKLMAYSFDDNGSERFEARIRNLETGELLPDTIPGTLSSLVW
                     TANNDAILYGLANENWRTDNVHVHYLGTPVSEDVRIYKEADIGFGVGIGKTADDAYIV
                     IATGDNETSEVYLLPADEPGAEPMLVSARKKGREYSVDVREKTLYIHTNDHHANFRIA
                     TASLDAPGEWKTLIPGSDDNYITGLSVFRDYFVLESREKGVDQVDVRQYDAPLTPGRI
                     EFPEATYVAGLGDNPEYHQDKLRLDYESMVTPDTVFDYDLAGGKLETLKVQEIPSGYN
                     HDEYQTELVHMPSRDGTPVPVSLVYKKGTPRDGSAPMHLYVYGSYGYRVPPGFSTTRL
                     SLVDRGMIYAIAHVRGGDDLGRAWYLAGKTDQRKNTFNDFIDVAKGLIAAKYTSAGKI
                     SIEGRSAGGQVMGAVYNQAPELWGAVLAGVPFVDVINTMVDKDLPLTPGEWPEWGNPI
                     TDKAAFDYMLSYSPYDNVTAKAYPPMLVSAGLNDPRVTYWEPAKWVAKLRATRTNDAT
                     LLLRTNMGAGHAGKSGRWGALREDAEEFAFVLTQLGVEK"
     gene            2666..4492
                     /locus_tag="VN034_05710"
     CDS             2666..4492
                     /locus_tag="VN034_05710"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HWV60126.1"
                     /translation="MSSAIHAERLARVRAELAARGLDGFVVPISDEHMSEYVGDYAQR
                     MAWLTGFGGSAGTAAVLPEKAAVFVDGRYTVQVRDQVDGSLFDYVGVPQSSVAEWLGA
                     NVSAGQKVGYDPWLHGIDWARGLEKALAEKGASLVAVDANPLDAAWDDRPAPSDAVVT
                     VHDAKLAGQGAVDKREIIADWLKAKGLDTTVMTALDSIAWTFNIRGEDVSHTPVGLAF
                     ALLHADATADLFIAPEKLTDAVRAHLGNSVRIHDRDAFEAALAGLADKKVAVDPDRAV
                     AAIFTALEGAGAKIERHRDPAVLPKAIKNETELDGTRAAHVRDGVAVSRFLKWMEAAA
                     PQGGLDELGAAAKLREFRDASGALRDLSFDTISAAGPNGALPHYKVDETTNRAIETGT
                     LYLVDSGGQYSDGTTDITRTIAIGTPTPEMRRRFTQVLKGHIALAVARFPKGTRGSQL
                     DILARQYLWADGVDYAHGTGHGVGTYLAVHEGPQRIAKPSGGQAGTEEPLHAGMILSN
                     EPGYYKAGHFGIRIENLVIVVPQSIDGAEEDMLGFETITFAPIAQNLVDVALLSGAEA
                     DWLDAYHTEVLAKLGVGMAGEEREWLEAACAPLDRSPAALAA"
BASE COUNT          796 a         1410 c         1488 g          799 t
ORIGIN      
        1 gcttcaagat cggggatcat ggagcgcgcc gtgatcgtga gcgcaaaacg acgttcgcgc
       61 ctgtcccggc ccggcaccaa ttctatcaag ccttcgcgta ccatcgtctg caacgtctgg
      121 gtggcggcgg gctgggtgaa accgagcgct gcgacgcatt cgccgaccga ggccgactcg
      181 cgcgccatga gcaaccgaaa ataagggtag aagcgcggcc ggaagttcag gccggcgtcg
      241 cgatacgcct gctcgacggc gtcgtcgagc gccgccacca gcaggcgcaa ccgcgttccc
      301 aatcccgatc gatagtccat atataagcac ttatatattt tctgcctgca aggcaacccg
      361 gaaggccgcc ggcgcgcccg cgcgccggcg atcagtcgtc gatgcgggta attgcggcta
      421 tttttccacc ccgagctgcg tcagcacgaa ggcgaattcc tcggcatcct cgcgcagcgc
      481 cccccaacgg cccgacttgc ccgcatggcc cgcgcccatg ttggtgcgca gcagcaaggt
      541 cgcgtcgttg gtgcgcgtcg cgcggagctt cgcgacccat ttggcgggct cccaataagt
      601 cacgcgcggg tcgttgagcc ccgccgacac gagcatcggc ggataggctt tcgcggtcac
      661 attgtcgtag gggctgtagc tcagcatata gtcgaacgcc gccttgtcgg tgatcggatt
      721 gccccattcg ggccattcgc cgggggtcag cggcaggtcc ttgtcgacca tcgtgttgat
      781 cacatcgacg aagggcacgc ccgcgagcac cgcgccccac agttcgggcg cctgattata
      841 gacggcgccc atcacctgcc cgcccgccga acggccctcg atcgaaatct tgcccgcgct
      901 cgtatatttg gcggcgatca gccccttggc gacgtcgatg aaatcgttga acgtgttctt
      961 gcgctggtcg gtcttgccgg cgagatacca ggcgcggccg aggtcgtcgc cgccgcggac
     1021 gtgcgcgatc gcatagatca tgccgcgatc gacaaggctg aggcgcgtcg tcgagaagcc
     1081 cggcgggacg cgatacccat aagagccata gacataaagg tgcatcggcg cgctgccgtc
     1141 gcgcggcgtg cccttcttgt aaacgagcga caccggcacc ggcgtgccgt cgcgtgacgg
     1201 catatgaacc aattcggttt gatattcgtc atgattataa ccggatggaa tctcctgaac
     1261 cttcagcgtt tcgagcttgc cgcccgcgag gtcgtaatcg aacaccgtgt cgggggtgac
     1321 catcgattca tagtcgagcc gtagcttgtc ctgatgatat tcgggattgt cgccgagccc
     1381 cgcgacataa gtcgcctcgg gaaactcgat ccggccggga gtcagcggcg cgtcatattg
     1441 gcggacatcg acctgatcga cacccttttc gcgcgattcg agcacgaaat agtcgcggaa
     1501 gaccgagagg ccggtgatgt aattgtcgtc cgatccgggg atcagcgttt tccattcgcc
     1561 gggcgcgtcg aggctggcgg tggcgatgcg gaaattggcg tgatggtcgt tggtgtggat
     1621 gtagagcgtc ttttcgcgca catcgacgct atattcgcgg ccctttttgc gcgccgacac
     1681 cagcatcggt tcggcgcccg gctcgtcggc ggggagcaga tagacttcgc tcgtctcgtt
     1741 gtcgccggtc gcgatcacga tataggcgtc gtcggcggtc ttgccgatac cgaccccgaa
     1801 accaatgtcc gcttccttat aaatgcggac atcctcgctg accggggtgc cgaggtaatg
     1861 gacatgcaca ttgtcggtgc gccaattctc gttcgcgaga ccgtagagga tcgcgtcgtt
     1921 gttcgcggtc cagacgagcg acgacaatgt gccggggatg gtgtcgggca gcagctcgcc
     1981 cgtttcgagg ttgcggatgc gcgcctcgaa gcgttcggag ccattgtcgt cgaacgaata
     2041 ggccatcagc ttgccgttcg ggctgaccga cagttcggcg aggcggaaat aatccttgcc
     2101 cgcggccata gcgacctcgt cgaggattag ctgggtgtcg ccgacgcccg acgccggctt
     2161 gcgataccat ttcttatatt cggcgccctc ttcatattcg acccaataga cccagtcgcc
     2221 gtccttctgc ggcaccgagg aatcggcttc cttgatacgg cccttcattt cctgaaacag
     2281 cgtctcgacc aattgcgcgt ggggtttcat cgccgcatcg aaccaggcat tctcggcctt
     2341 cacatagtcg agcacatcct tgtcatcgat caccggatag ctttcgtcct tcagccagtg
     2401 gtacggatcg gacagcgttt tgccgtgcag cgtcgtctcg tgcgggcgct tttccgcgac
     2461 gggggcggcg gatagggcag gggtcgggct ggtcaatgca gggtctttct ctctggcgaa
     2521 aagctggggg gcgaacagtg agggcgatgc gacaagcggg gtggcaattg cggccagaaa
     2581 catccaaata gcgcgcatca caaaatcccc cgcggtgtcg cccgattggg cgggatcgct
     2641 gcgatgtagg aataaggaac gtctgatgtc cagcgccatc catgcagaac gcctcgcgcg
     2701 tgtccgtgcc gaacttgccg cccgcggcct cgacggtttc gtcgtgccga tcagcgacga
     2761 gcatatgagc gaatatgtcg gcgactatgc ccagcgcatg gcgtggctga cgggcttcgg
     2821 cggatcggcg gggaccgcgg cggtgctgcc cgaaaaggcg gcggtgttcg tcgacgggcg
     2881 ctatacggtg caggtccgcg accaggtcga cgggtcgctg ttcgactatg tcggcgtgcc
     2941 gcagtcgagc gtcgccgaat ggctcggcgc gaatgtcagc gccgggcaga aggtcggcta
     3001 tgacccgtgg ctgcacggga tcgactgggc gcgggggctg gagaaggcgc tcgcggagaa
     3061 gggcgcgagc ctcgtcgcgg tcgacgcgaa cccgctcgac gccgcatggg acgatcggcc
     3121 cgcgccgagc gatgcggtgg tgacggtcca tgatgcaaag ctcgcggggc agggcgcggt
     3181 cgacaagcgc gagatcatcg ccgactggct gaaggcgaag gggctcgaca cgacggtgat
     3241 gaccgcgctc gattcgatcg cgtggacctt caacattcgc ggggaagacg tcagccacac
     3301 gccggtgggg ctcgcgttcg cgctgcttca cgccgatgcg accgccgacc tgttcatcgc
     3361 acccgaaaag ctgaccgacg cggtgcgcgc gcaccttggc aacagcgtgc gcatccacga
     3421 ccgcgacgcg ttcgaggccg cgcttgcagg actcgctgac aagaaggttg cggtcgatcc
     3481 cgaccgcgcg gtcgcggcga tcttcaccgc gctcgaaggc gcgggcgcaa agatcgagcg
     3541 tcaccgcgac cccgcggtac tgcccaaggc gatcaagaac gagaccgagc tcgacggcac
     3601 gcgcgccgcg catgtccgcg acggcgtcgc ggtgtcgcgt ttcctcaaat ggatggaagc
     3661 ggccgcgccg cagggcgggc tcgacgaact cggcgccgcg gcgaagctgc gcgaatttcg
     3721 cgatgcaagc ggcgcgctca gggatctgtc gttcgacacg atctcggccg ccggccccaa
     3781 cggcgcgctg ccgcattaca aggtcgacga aacgaccaac cgcgcgatcg agacgggcac
     3841 gctctatctc gtcgattcgg gcggacaata ttccgacggg acgaccgaca tcacgcgcac
     3901 gatcgcgatc ggcacgccta cgcccgaaat gcggcgccgc ttcacgcagg tgctgaaggg
     3961 ccatatcgcg ctcgcggtcg cgcgcttccc caagggcacg cgcggcagcc agctcgacat
     4021 tctggcgcgc caatatctgt gggccgacgg ggtcgattat gcgcatggca ccggccacgg
     4081 cgtcggcacc tatctggcgg tccacgaagg gccgcagcgc atcgccaagc cgtcgggcgg
     4141 gcaggcggga accgaagaac cgctccacgc cgggatgatc ctgtcgaacg agcccggcta
     4201 ttacaaggcg ggccatttcg gcatccgcat cgaaaatctc gtcatcgtcg tgccgcaaag
     4261 catcgacggc gccgaggagg atatgctggg gttcgagacg atcaccttcg caccgatcgc
     4321 gcaaaatctg gtcgacgtgg cgctgctgtc gggtgccgag gcggactggc tcgatgctta
     4381 tcataccgag gtgctggcga agctcggcgt gggcatggcg ggggaggagc gcgaatggct
     4441 cgaagccgcg tgcgcgccgc tcgaccgcag ccccgccgcg ctcgcggcct gag
//
LOCUS       DATMSU010000224         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_13391, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000224 DATMSU010000000
VERSION     DATMSU010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13391"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..506
                     /locus_tag="VN034_05715"
     CDS             66..506
                     /locus_tag="VN034_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HWV60127.1"
                     /translation="MTPLRRILIVGFIAFLAAVAGVFVGRMLVDTPRADETELHALLH
                     REAKLSAGQERKLDAIETRFAARREALELEMRAANIRLAQAIEAEHGYGPRVTKAIDE
                     THQVMGELQKETLQHLFAMRAVLDEDQAAMFDRTVVKALTADAR"
     gene            503..1069
                     /locus_tag="VN034_05720"
     CDS             503..1069
                     /locus_tag="VN034_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWV60128.1"
                     /translation="MSVELAQCSDRELAALAHTRRDDIYRELLARYKAPVFRLIRHHV
                     GDADEAMDLSQETFVSAFAAIERYDGDRPFRIWISRIAINKCRDWARRRAVRSFFTRA
                     LPLEKAHDVAVEGPAPDVHAADREELARVRKAISGLPHNLREVLILRGVEELSQSETA
                     ELLQVSEKTVETRLYRARTKLRAMLGEN"
     gene            1127..>2214
                     /locus_tag="VN034_05725"
     CDS             1127..>2214
                     /locus_tag="VN034_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HWV60129.1"
                     /translation="MFMQIDRRGFVNGALGGGALAALAAWYPAWAQPVSPGIASTLPT
                     VSGNDIRLRIARRTMRIDGKVSRAIGINGTIPGPLVRLREGQTARLTVVNDLDEDSSI
                     HWHGLILPFQMDGVPGVSFPGIKPRSTFVYEFPVVQSGTYWYHSHSGLQEQLGHYGPI
                     VIDPAGADPIGYDREHVIVLSDHSQLSPEAIFRKLKVNPGHFNMQRQTLSGLLAGKDQ
                     PLKERVEWGAMRMDPTDIADVSGSTYQFLVNGYGPRDNWTALFAPGERVRLRIVNASA
                     MSIFNVRIPGLRMTVVQADGLNVVPTEIDEFQIGVAETYDVIVTPADDRAYTIVAEVN
                     DRSGMARATLAPRAGTREVKASRHILRDV"
BASE COUNT          390 a          714 c          742 g          368 t
ORIGIN      
        1 agcccgctta cgccgctcgt ccccgcgagc ccgctggcgc cgtcgacgct gctggattcg
       61 cgctgatgac gccgctgcgc cgcatcctga tcgtcggctt tatcgccttt ctggccgcgg
      121 tggcgggcgt gttcgtcggc cgcatgctcg tcgacacgcc gcgggccgac gagaccgagc
      181 tgcacgcgct gcttcaccgc gaggcgaaat tgtcggccgg acaggagcgc aagctggacg
      241 cgatcgagac gcggttcgcc gcgcgtcgcg aagcgctcga gctggagatg cgtgcggcga
      301 atattcgcct cgcgcaggcg atcgaggccg agcatggcta tggcccgcgc gtcaccaagg
      361 cgatcgacga aacgcatcaa gtgatggggg aattgcagaa ggagacgctc cagcatctgt
      421 tcgccatgcg cgccgtgctc gacgaggatc aggccgcaat gttcgacagg accgtggtca
      481 aggccctgac cgccgatgcg aggtgagcgt cgagctagcg caatgtagcg accgcgagct
      541 cgccgcgctc gcgcacaccc ggcgggacga catctatcgc gagctgctgg cgcgttacaa
      601 ggcgccggtc ttccgcctca tccgtcacca tgtcggcgat gccgacgagg cgatggacct
      661 gtcgcaggaa accttcgtat ccgcctttgc ggcgatcgag cgttacgacg gcgaccggcc
      721 gttccggata tggatctcgc gcatcgcgat caacaaatgc cgcgactggg cacggcggcg
      781 cgcggtgcga tcctttttca cgcgcgcgct gccgctcgaa aaggcgcatg atgtggcggt
      841 cgagggaccc gcgcccgacg tccacgccgc cgaccgcgaa gaacttgcgc gggtgcgaaa
      901 ggcgatatcc ggattgccgc acaatttgcg cgaggtgctg atcctgcgcg gcgtcgagga
      961 gcttagccag tcggaaaccg ccgaattgtt gcaggtcagc gaaaagaccg tcgaaacgcg
     1021 tctttaccgg gcccggacca agctgcgggc gatgctcggc gaaaattgac gtgagggatg
     1081 cgcctctgct tcgcgtatga ccctggagag aaacaggatt accaggattt ttatgcagat
     1141 cgacaggcgc gggttcgtga acggggcatt gggcggaggc gcgctcgccg cgctggcggc
     1201 atggtatccg gcctgggcgc agccggtatc gcccggcatc gcctcgacgc tgccgacggt
     1261 ttcggggaac gatatcaggc tgcgcatcgc aaggcggacg atgcgtatcg acggcaaggt
     1321 cagccgggcg atcgggatca acggcaccat cccggggccg ctggtgcggc tgcgcgaagg
     1381 gcagacggcc cgcctcaccg tcgtcaacga tctcgacgag gacagctcga tccactggca
     1441 cgggctgatc ctgccgttcc agatggacgg cgtgccgggg gtcagctttc ccggcatcaa
     1501 gcccaggtcg accttcgtct acgaattccc cgtcgtccag tcgggcacct attggtacca
     1561 cagccactcg ggcctgcagg aacagctcgg gcattacggc ccgatcgtga tcgatcccgc
     1621 gggcgccgac ccgatcggat atgaccgcga gcatgtgatc gtcctgtcgg accacagcca
     1681 gctgtcgccc gaggcgatct ttcgcaagct caaggtcaat ccggggcatt tcaacatgca
     1741 gcggcaaacg ctgtcggggc tgctcgcggg caaggaccag ccgctcaagg agcgcgtcga
     1801 atggggcgcg atgcggatgg atccgaccga catcgccgac gtcagcggat cgacctatca
     1861 attcctcgtc aacggctatg gtccgcgcga caattggacc gcgctgttcg cgccgggcga
     1921 acgcgtccgg ctgcgcatcg tcaacgcatc ggcgatgtcg atcttcaacg tccgtattcc
     1981 cgggcttcgc atgaccgtgg tgcaggccga cggcctcaac gtcgttccga ccgagatcga
     2041 tgaattccag atcggtgtcg ccgaaaccta tgacgtcatc gtcacccctg ccgacgaccg
     2101 cgcctatacg atcgtcgccg aggtcaacga ccggtcgggc atggcgcgcg cgacgctggc
     2161 gccgcgcgct gggacgaggg aggtcaaggc ctcgcgtcat atcctgcggg acgt
//
LOCUS       DATMSU010000225         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_4248, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000225 DATMSU010000000
VERSION     DATMSU010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4248"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VN034_05730"
     CDS             <1..427
                     /locus_tag="VN034_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60130.1"
                     /translation="AGAPAEPAPNYKLFSTQMKSGPSDHAIFAEGVKTDGAGCVRGTF
                     QITLRSAEGIIYYLGETLREGNSMPAIDGIPLLSIKRQRPDDIFVQARFRGENWYVPL
                     DAQLKTSRTSQIVALVQQLINLQKLSKDKPTTQSIRILQ"
     gene            513..>1885
                     /gene="purH"
                     /locus_tag="VN034_05735"
     CDS             513..>1885
                     /gene="purH"
                     /locus_tag="VN034_05735"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWV60131.1"
                     /translation="MSDLIPVRRALLSVSDKAGLAELAAALVRHGVELVSTGGTAKAL
                     REAGHDVLDVSDLTGFPEMMDGRVKTLHPTVHGGILAVRDDAAHVASMDEHGIGAIDL
                     VVVNLYPFAATVAKGAARDEIIENIDIGGPAMVRSSAKNHAFVNIVTEPEDYAAVIAE
                     MDANGGATTLALRKRLAATAFAHTATYDSMIAQWFAFADQGKMFPDTLPLTAKLANEL
                     RYGENPHQKAALYLPAGPAARGIAQAEQVQGKELSYNNINDADAALELVAEFREADPT
                     CVIVKHANPCGVATAATIAEAYDAALKCDDVSAFGGIIAVNRPLDGATAELISGIFTE
                     VVCAPDADADARAVFAKKKNLRLLLTGELPDPARGGLMMKTIAGGWLAQSRDAGQIGG
                     QIGIGDLRVVTERAPTDEELTDALFAWTVAKHVKSNAIVYAKGGSTAGIGAGQMNRRD
                     SARIAAV"
BASE COUNT          358 a          634 c          600 g          293 t
ORIGIN      
        1 agcgggtgca cccgcagaac ccgcccccaa ttacaagctt ttttcgaccc agatgaaaag
       61 cgggccgtcc gatcatgcga tattcgccga aggggtgaaa accgacggcg cgggctgtgt
      121 gaggggcacg tttcagataa ccctccgctc ggccgaaggc atcatctatt atctaggcga
      181 aacgttgcgc gaggggaatt ccatgcccgc gatcgatggc attcccctgc tctcgatcaa
      241 gcggcagcgt cccgacgata tattcgtgca ggcacggttt cgcggcgaga attggtatgt
      301 tccgctcgac gcgcaactca agacgagccg gacgtcgcaa atcgtcgcgc tcgtccagca
      361 attgatcaat ctgcaaaagc tgtcgaagga caaaccgacc acgcaatcga tccgcatatt
      421 gcaataagcc gagcgggtcc gtggttgcgc aaaagggggc aaacgcctag ggggcggcgc
      481 gtctccattc ctttgcgaaa gccacgctcc ccatgtccga cctgattccc gtccgccgtg
      541 ccctcctgtc cgtcagcgac aaggcggggc tcgccgagct tgccgccgcg ctcgtccgcc
      601 atggcgtcga actggtttcg accggcggca ccgcgaaggc gctgcgcgaa gccggccacg
      661 acgttctcga cgtgtcggat ctcaccggct ttcccgagat gatggacggc cgcgtcaaga
      721 cgctgcaccc gacggtccac ggcggcatcc tcgcggtgcg cgacgatgcg gcgcatgtcg
      781 cctcgatgga cgaacatggc atcggcgcga tcgatctggt ggtggtcaat ctctacccct
      841 tcgccgcgac ggtcgccaaa ggcgccgcgc gcgacgagat catcgagaat atcgacatcg
      901 gcggccccgc gatggtgcgc tcgtcggcga agaaccacgc gttcgtcaat atcgtcaccg
      961 agcccgagga ttatgccgcg gtgatcgccg agatggacgc gaacggcggc gcgacgacgc
     1021 tggccttgcg caagcggctc gccgcgaccg cctttgccca caccgcgacc tatgacagca
     1081 tgatcgcgca gtggttcgcc tttgccgatc agggcaagat gttccccgac acgctgccgc
     1141 tgaccgcgaa gctcgccaat gaactccgct atggcgagaa tccgcaccag aaggcggcgc
     1201 tctatctgcc cgccggcccc gccgcgcgcg gcatcgcgca ggccgagcag gtgcagggca
     1261 aggaactgag ctataacaat atcaacgacg ccgacgcggc gctcgaactc gtcgccgaat
     1321 tccgcgaggc cgatccgacc tgcgtcatcg tcaagcacgc caacccgtgc ggcgtcgcga
     1381 ccgccgcgac gatcgccgag gcctatgacg cggcgctgaa gtgcgacgat gtctcggcgt
     1441 tcggcgggat catcgcggtc aaccggccgc tcgatggcgc gaccgccgaa ctgatcagcg
     1501 gcatcttcac cgaagtcgtc tgcgcccccg atgccgacgc cgacgcgcgc gcggtgttcg
     1561 cgaagaagaa gaacctccgc ctgctgctga cgggcgaact gcccgatccg gcgcgcggcg
     1621 ggctgatgat gaagacgatc gccggcggct ggctcgcaca gagccgcgac gctggtcaaa
     1681 ttgggggcca gatcggcatc ggcgacctca gggtcgtcac cgagcgcgca cccaccgacg
     1741 aggagctgac cgacgcgctc ttcgcatgga ccgtcgccaa gcatgtgaag tcgaacgcga
     1801 tcgtctatgc caagggcggc tcgaccgcgg gcatcggcgc ggggcagatg aaccgccgcg
     1861 acagcgcgcg catcgccgcg gtgaa
//
LOCUS       DATMSU010000226         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_164892,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000226 DATMSU010000000
VERSION     DATMSU010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164892"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /gene="mutL"
                     /locus_tag="VN034_05740"
     CDS             complement(<1..989)
                     /gene="mutL"
                     /locus_tag="VN034_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWV60132.1"
                     /translation="MSIRRLPEKLVNRIAAGEVVERPAAALKELVENAIDAGSTRISV
                     RIAEGGISRLEVEDDGCGMTAADMALALERHATSKLPDDAIEDVTTLGFRGEALPSIA
                     SVARLTLESRIAGGDGWKRVVDNGAVVAEGPTALGKGTRVMVEDLFARVPARRKFLRS
                     GRSEYAACLDVVKRLAMARPDIGFIVEHDGRRVLDVQGGQDQLTRVAALTQRDLAANS
                     IGVDIDRGDVHLGGVISLPTYNRGIADHQYLFVNGRPVKDRLLVGALRGAYADLLARD
                     RHPVVALFLDVPTSEVDVNVHPAKTEVRFRDPQLVRGMIVGGLRRALDEHGFR"
     gene            1183..2229
                     /locus_tag="VN034_05745"
     CDS             1183..2229
                     /locus_tag="VN034_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWV60133.1"
                     /translation="MSFFSRWLKFNSQDMAIDLGTANTVVYVRGKGIVLNEPSVVAVE
                     TLNGIKRVKAVGDDAKLMMGKTPDSIEAIRPLRDGVIADIDVAEQMIKHFITKVHGGK
                     PNAFRSPEIVICVPSGSTSVERRAIRDAASNAGASEVWLIEEPMAAAIGADMPVTEPI
                     GSMVVDIGGGTTEVAVLSLRGLAYTTSVRAGGDKMDEAIVSYVRRHHNLLIGESTAER
                     IKKDFGIARIPADGTGLTIHIKGRDLVNGVPKEISINQAQIAEALSEPIGTIVEGVRI
                     ALENTAPELAADIVDQGIVLTGGGALIAELDELLRDATGLPVTVAEDPLTCVAIGTGR
                     AMEDPAFRGVLQQA"
     gene            2247..>2734
                     /locus_tag="VN034_05750"
     CDS             2247..>2734
                     /locus_tag="VN034_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWV60134.1"
                     /translation="MVRPAHRRPGQSRKAQYGLFAAYVIAVTGAVAGLLLAILSVVDP
                     VGFAQLRLASQEIAAPIARVTRSVTGSLSGIDDSVGAYVGAGTQNRRLRKELAETRRQ
                     LVATSSLQEENRQLKALLKLQETDKTALANGYLLTSTSTSAKRIALLSIGRNLGVAAG
                     QPV"
BASE COUNT          490 a          951 c          862 g          431 t
ORIGIN      
        1 ctgcggaagc catgttcgtc gagcgcgcgg cgaaggccgc cgacgatcat cccgcgaacg
       61 agctgcggat cgcgaaaacg cacctcggtc ttcgccggat ggacgttcac atcgacctcg
      121 ctcgtcggca cgtcgaggaa cagcgcgacg accggatggc gatcgcgcgc gagcaggtcg
      181 gcataggcgc cgcgcagcgc gccgacgagc agccggtcct tcaccgggcg cccgttgacg
      241 aacagatatt gatggtcggc gatgccgcgg ttgtaggtcg gcagactgat cacgccgccg
      301 agatgcacgt cgccgcgatc gatatcgacc ccgatgctgt tcgcggcgag atcgcgctgc
      361 gtcagcgccg cgacgcgagt cagctggtcc tgcccgccct gcacgtcgag cacgcggcgc
      421 ccgtcatgct cgacgatgaa accgatatcg gggcgcgcca tcgcaagccg cttcacgacg
      481 tcgaggcacg cggcatattc gctgcgcccg ctgcgcagga atttgcgccg cgcgggaacg
      541 cgtgcgaaca aatcctcgac catcacgcgc gtgcccttgc ccagcgccgt cggcccttcg
      601 gccaccaccg cgccattgtc gacgacacgc ttccagccgt cgccacccgc gatacggctt
      661 tcgagcgtca gccgtgcgac gctcgcgatc gagggcagcg cctcaccccg gaaacccagc
      721 gtcgtcacat cctcgatcgc gtcatcgggc agcttcgacg tcgcatggcg ttcgagcgcc
      781 agcgccatgt cggcagcggt cattccgcag ccgtcgtcct cgacttcgag gcgcgaaatc
      841 ccgccctcgg cgattcgcac cgaaatgcga gtcgaaccgg catcgatcgc attttcaacc
      901 agttccttga gcgctgcggc aggtctttcg accacttcac cggctgcgat ccgatttact
      961 agcttttccg gcaggcgacg tattgacatg gccgctctcc tagcgcagtc gaacgcaaat
     1021 cgcgacccat ccgcacagcc ctgatcattt tgtccagcca ccccccgtgg acgagccgga
     1081 atctccccct gattgtagct gtcgccggcc ggacgcgcgg gtgaatcacg acagggggcg
     1141 tgcccgtcag gggcgcggct tcgatgacag gaagcagtat cgatgtcctt cttttctcgc
     1201 tggcttaaat tcaactccca agacatggca atcgacctcg gcaccgcgaa caccgtggtc
     1261 tatgtgcgcg gcaagggcat cgtgctgaac gagccgtcgg tcgtcgcggt cgagaccctg
     1321 aacgggatca agcgggtcaa ggcggtcggc gacgacgcca agctgatgat gggcaagacc
     1381 cccgacagca tcgaggcgat ccgtccgctg cgcgacggcg tcatcgccga catcgacgtc
     1441 gccgaacaga tgatcaagca cttcatcacc aaggtgcacg gcggcaagcc caacgcgttc
     1501 cgcagccccg aaatcgtgat ctgcgtcccc tcgggctcga ccagcgtcga acgccgcgcg
     1561 atccgcgacg ccgcatcgaa tgcgggcgcc agcgaagtgt ggctgatcga ggaaccgatg
     1621 gcggccgcga tcggcgccga catgccggtg accgaaccga tcggttcgat ggtcgtcgac
     1681 atcggcggcg gcacgaccga agtcgcggtg ctcagcttgc gcggtctcgc ttacaccacg
     1741 tcggtccgcg cgggcggcga caagatggac gaagcgatcg tctcctacgt ccgccgccac
     1801 cataatctgc tgatcggcga atcgaccgcc gaacggatca agaaggattt cggcatcgcg
     1861 cgcatccccg ccgacggcac cggcctgacg atccacatca agggccgcga tctcgtcaac
     1921 ggcgtgccca aggaaatctc gatcaaccag gcacagatcg ccgaagcgct gtccgagccg
     1981 atcggcacga tcgtcgaggg cgtgcgcatc gcgctcgaga ataccgcccc cgaacttgcc
     2041 gccgacatcg tcgaccaggg catcgtgctc accggcggcg gcgcgctgat cgccgaactc
     2101 gacgaactgc tccgcgatgc gaccggcctg ccggtcacag tggccgagga tccgctgacc
     2161 tgcgtcgcga tcggcaccgg ccgcgcgatg gaagaccccg ccttccgcgg cgtgttgcag
     2221 caggcctgat cgagagcctc tgaaccatgg tccgcccggc acatcggcgt ccggggcaat
     2281 cccgaaaggc ccaatatggc ctgttcgctg cctatgtcat cgcggtgacc ggggcggtgg
     2341 cgggtctgct gttggcgatc ctgtcggtcg ttgaccccgt cggcttcgcc cagttgcgct
     2401 tggcgagcca ggaaatcgcc gcgccgatcg cgcgcgtgac gcggtcggtg acgggatcgc
     2461 tttcgggcat cgacgatagc gtcggcgcct atgtcggcgc gggcacgcaa aaccggcggc
     2521 tgcgcaagga actcgccgag acgcgccgcc agcttgtcgc gaccagctcg ctgcaggaag
     2581 agaaccgcca attgaaggcg ctgctcaagc tgcaggaaac cgacaagacc gcgctcgcca
     2641 acggctatct gcttacctcg acctcgacga gcgccaagcg catcgcgctg ctcagcatcg
     2701 gacgcaatct cggcgtcgcc gcgggccagc cggt
//
LOCUS       DATMSU010000227         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_164940,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000227 DATMSU010000000
VERSION     DATMSU010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164940"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VN034_05755"
     CDS             complement(<1..459)
                     /locus_tag="VN034_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60135.1"
                     /translation="MRLPKNSFVFAPLAAIALAFSPPAFAQSDGQSPQPAIEAGNYPY
                     QLFLPRGYTADSKASWPLLIFLHGSGERGDDVAKVKVHGPPKIADRSPDFPFVTVSPL
                     LGADQDWDVAKLDRLLDHVTKTLRIDPTRIYLTGLSRGGHASWRWAAAEPH"
     gene            complement(480..1295)
                     /locus_tag="VN034_05760"
     CDS             complement(480..1295)
                     /locus_tag="VN034_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWV60136.1"
                     /translation="MNLRRWSVTLLAALVALVTIAPLLWMVSVSLMARGEAAQFPPPL
                     WPASPTLENYHLLFGSFGVGRFLFNSILISTLATGLALLFTIPAGYAFAKLRFKGRDA
                     TFRLLVAALVVPGQIGMLPLFLELKAMGLVNSYAGALVPWLAGIFGIFLVRQYCLSIP
                     DEMLEAARIDGASEGQILRRIVLPILTPIIVTLALYVFLSSWNDFMWPLIILADQDLA
                     TLPVALAVNSRQNVQDYEIMMAGAVVTTLPVLILFLALQRYYLSGLLTGSVKG"
     gene            complement(1292..2179)
                     /locus_tag="VN034_05765"
     CDS             complement(1292..2179)
                     /locus_tag="VN034_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWV60137.1"
                     /translation="MGRTGEARAGWAMSSPALAAILLFFVLPAIASLFLSFTDFDIYA
                     LADIGNLRFVGFQNYERLLENPLFWKAMTNTLLFVAVGTPFIVALSLFAAMLVNSRWL
                     TWRPVWRVALFAPYVTTLVATAVVWRYLLHTRYGLLNYILSFFGVPPVDWLGSPTASL
                     PAILLFVGWKTFGYNMIIFLAALQTVPRELDEAARIDGAEWFTRLRHVTLPAIAPTVL
                     LVSVLTVAGMFQLFAEPYVMTQGGPAQSTVTILYFMYEEGFKWWNLGSGAAVAFLLFL
                     CILAVTLVQLRLAKRSGAI"
     gene            complement(2185..>2413)
                     /locus_tag="VN034_05770"
     CDS             complement(2185..>2413)
                     /locus_tag="VN034_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWV60138.1"
                     /translation="LMRDPAVAPFRAQLEHATALPKVPEWERIVTEMQIVAERMVRGQ
                     YSVDEAAKEIDARADRLLEKRRWMLDKGRAL"
BASE COUNT          494 a          750 c          818 g          351 t
ORIGIN      
        1 gtgcggctcg gccgcggccc agcgccagct cgcatggccg ccgcggctga gcccggtgag
       61 atagatgcgc gtggggtcga tgcgcagcgt cttggtcaca tgatcaagca gccggtcgag
      121 cttcgcgacg tcccagtcct gatcggcgcc gagcagcggc gagacggtga cgaaggggaa
      181 atcggggctg cggtcggcga tcttcggcgg gccgtgcacc ttgaccttcg cgacatcgtc
      241 gccgcgctcg cccgatccgt ggaggaagat cagcagcggc cagctcgcct tggaatcggc
      301 ggtgtagccg cgcggcagga acagctggta ggggtagttg cccgcctcga tcgcgggttg
      361 cgggctttgt ccgtcgcttt gagcaaaggc tggcggggag aaagccaacg cgatggccgc
      421 gagcggagca aagacgaacg agtttttggg cagcctcaac gacttgctcc tcataaattt
      481 catcccttca cgctcccggt caaaagcccg ctcagatagt aacgctgcag cgcgaggaag
      541 aggatgagga cggggagggt ggtgacgacc gcgccggcca tcatgatctc ataatcctgc
      601 acattctggc ggctattcac cgcgagcgcg acgggcagcg tggcaagatc ctgatcggcg
      661 aggatgatca gcggccacat gaaatcgttc cagctcgaca ggaagacata gagcgcgagc
      721 gtgacgatga tcggagtcag gatcggcagc acgatgcggc gcaatatctg cccctcgctc
      781 gcgccgtcga tgcgcgccgc ctcgagcatc tcgtcgggga tcgacaggca atattggcgg
      841 acaagaaaga tgccgaaaat ccccgcgagc cacggtacga gcgcgccggc atagctgttg
      901 acgagcccca tcgccttgag ttcgaggaac agcggcagca ttccgatctg ccccggcacg
      961 acgagcgccg cgacgagcaa acggaaggtc gcgtcgcggc ctttgaagcg cagcttggcg
     1021 aaggcatagc ccgccggaat cgtgaaaaga agcgcaagcc ccgtcgcgag cgtcgaaatg
     1081 aggatgctgt tgaagaggaa gcggccgacg ccgaaactgc cgaagagcag gtggtaattt
     1141 tcgagtgtcg ggctcgcggg ccacagcggc ggcggaaatt gcgccgcctc gccgcgcgcc
     1201 atcaagctga ccgagaccat ccagagcaag ggcgcgatcg tgaccagcgc gacgagcgcg
     1261 gcgagcaggg tgacggacca gcggcggaga ttcatatcgc gccgctccgc ttggcgagcc
     1321 gcaattgcac cagcgtcacc gcgaggatgc agaggaagag caggaaggcg accgccgcgc
     1381 ccgacccgag gttccaccat ttgaaccctt cctcatacat gaaataaagg atggtgacgg
     1441 tcgactgcgc ggggccgccc tgcgtcatca catagggttc ggcgaagagc tggaacatgc
     1501 ccgcgacggt gagcaccgag acgagcagca ccgtcggcgc gatcgcggga agcgtgacgt
     1561 ggcgcagccg cgtgaaccac tccgcgccat cgatccgcgc cgcctcgtcg agttcgcgcg
     1621 gcaccgtctg caacgcggcg aggaagatga tcatattata gccgaaggtc ttccacccga
     1681 cgaagagcag gatcgcgggc agcgaggcgg tcgggctgcc gagccagtcg accggcggca
     1741 ccccgaagaa ggagaggata tagttgagca gcccgtaacg cgtgtgaagc aggtagcgcc
     1801 acaccaccgc ggtcgcgacg agcgtcgtca catagggcgc gaacagcgcg acccgccaca
     1861 ccggccgcca tgtcagccag cgcgaattga cgagcatcgc cgcgaacagc gacagcgcaa
     1921 cgatgaaggg cgtgccgacc gcgacgaaca gcaatgtgtt cgtcatcgcc ttccagaaca
     1981 gcggattttc gagcaggcgt tcgtaattct ggaagccgac gaagcgcagg tttccgatgt
     2041 cggcgagcgc gtaaatgtcg aaatcggtga agctcagaaa cagcgaggcg atcgcgggca
     2101 gcacgaagaa gagcaggatc gccgcgagcg ccggcgacga catcgcccaa cccgcgcgcg
     2161 cttcgcccgt ccggcccatc gcattcacag cgcccgcccc ttgtcgagca tccagcgccg
     2221 cttttcgagc aggcggtcgg cgcgcgcgtc gatctccttc gcggcctcgt cgacgctata
     2281 ttggccgcgc accatgcgtt cggcgacgat ctgcatctcg gtgacgatcc gctcccattc
     2341 gggcaccttg ggcagcgcgg tcgcatgctc gagctgggcg cggaaggggg cgaccgccgg
     2401 atcgcgcatc aat
//
LOCUS       DATMSU010000228         6534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_98743, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000228 DATMSU010000000
VERSION     DATMSU010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98743"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VN034_05775"
     CDS             <1..1076
                     /locus_tag="VN034_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWV60139.1"
                     /translation="TREAAARREPSYAQQYGLVAPVDVYPLYENALRASLGQSLAEAQ
                     DESGRIWSLFSEVAAANPHAWIRKGVSAADVTTASESNRPIAFPYNKLMVANASVNQG
                     AAFIVTSVAEARRRGIAEDRWIYIGNGAAAHEPNSPLQRDRYDQSASMAVSIRRTLAL
                     NAVDPGDLDVVELYSCFPCIPKLARRLIDWPVDRPATVFGGLTFGGGPIGNYMGHAIA
                     SMTDRLRETGGKGLLFANGGYATHNHSIVISSAPIPAASFPRSYDFQAEADAMRDDIP
                     ALARDHLGDATIESYTVLYRRDGSPKAGVIVARTPDNARTLAHVPPGDDITLRRLVDP
                     DADPVGAAGRIVASETLREWRFA"
     gene            1187..1843
                     /locus_tag="VN034_05780"
     CDS             1187..1843
                     /locus_tag="VN034_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWV60140.1"
                     /translation="MPSSQAKRRQGPGRPLTPLITREIAVRTSIELLDRKGLDGLSVQ
                     AVARELNVQAPSLYYHFKNRDELLHMIAKTLLVEVGRGEKPGSSWEERVVDLAVATRR
                     VMLCHAHAGPLMLRFFPRELMLGAYERGLSDCPYAPEHHLAILEALEKLTYGKALFDA
                     AAKAHHHAAMPPVDADRYPRLAEALAKSTVSDEALFVETLDVLLDGFRFRYGKKDRTQ
                     "
     gene            1840..2241
                     /locus_tag="VN034_05785"
     CDS             1840..2241
                     /locus_tag="VN034_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV60141.1"
                     /translation="MIEGGCNCGAVRYRAEGDPIVVAQCHCTNCRRQSGSAFSVNLLF
                     KGAAVSHEGDLAVYEDKDTFSGNAVLRKFCGRCGSPIFSEPTDGKGLLIIKAGTLDDP
                     APFVPTVSVWTSTALPWVSLPEGQYAFERNA"
     gene            complement(2269..3066)
                     /locus_tag="VN034_05790"
     CDS             complement(2269..3066)
                     /locus_tag="VN034_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWV60142.1"
                     /translation="MSDAPTVTVDHDGAVAVIRYANAPSGLISNKGAALLADRIGHEL
                     EREATRCLVLTGGEEGVFIRHADVSQIRRAGDAVASGAIRPEDFAASPFARIGHMLDA
                     SEKPVIAAINGVCMGGGLEIALACTLRIAASSPNYSIGLPEIRLGIFPGAGGIARLAR
                     LIGAHRARRFALEGAIVNAGAAAELGLIDELTADPVGRAIDLATIFAGRHPAAVAAIM
                     ELAAAEDDDSAIERSMIRFGELVGGSREIRHRLSAFAKGSRPLEDMD"
     gene            complement(3063..3803)
                     /locus_tag="VN034_05795"
     CDS             complement(3063..3803)
                     /locus_tag="VN034_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60143.1"
                     /translation="MAGRLDKKIIVLSGGTRGMGEAQVRGMVREGAHVVFGGRDRAAG
                     AAIEADLGGGAHYRPLDVTNASDWKAVIDFATSRFGRIDGLVNNAGVGATHALTEISD
                     EAWADIIAVNQTGVFFGMREGARAMLETGGGSIVNVASPAGTKAHAMLVPYSAAKAAV
                     IGMSRAAAKELAPHGIRVNTMIPGFFATRLLTETTNSQALERGAQLTPLGRVAQPDES
                     VGAMLYLLSDESSFVTGTQIVVDGGYTA"
     gene            4005..4787
                     /locus_tag="VN034_05800"
     CDS             4005..4787
                     /locus_tag="VN034_05800"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWV60144.1"
                     /translation="MSKGLFDLTGKVALVTGGNGGIGLGMAKGLAMHGASVVIWGNKA
                     EKNERAVAELEAFGGAVRCAQVDVADEAAVKAGIAAIVEEFGRIDQAIANAGIGIPRR
                     DMFEISQADFEKLQSVNVYGVFYTLREAAAHMIERGKSGDRGGSLVSIASTASIHGAA
                     RNEHYGATKGAVLSLSRAMAVELAPHAIRVNSILPGWTRSDMTERLQGWDKFNENVIS
                     RAPVGRWGDGEDFSAIAVYLASKHSSFHLGDAIVIDGGYTIY"
     gene            5122..>6534
                     /locus_tag="VN034_05805"
     CDS             5122..>6534
                     /locus_tag="VN034_05805"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWV60145.1"
                     /translation="MAFRKAALWAVMAATALNSSVVMAQEASEGEAADSAAPVEGDIV
                     VTAQRREERLVDVPISVSAVSSAAIDRAQVRTVSDIATVVPNIQINETIGNSYGPLIT
                     IRGLSPSADTSLARDQPVGLYIDGVPIGKSTGAAFDTVDLERVEVLRGPQGTLYGKNT
                     IGGAVNLITRKPSGEFGGQFLAGAGSWGQFNQRISVDLPEVAGFSAKVGLITKLDGGY
                     YRNSATGKNFGEEELWAARGDLLWRPSDSFSALYSYDISDSKGTPNQLAATAVTGSGA
                     TAFVNALMAPYVVDGRTREIGAQSALRSDYRTNGHALTLEWEPGLGDLTLKSITARRT
                     AITRSASDFDGSPLDLLRIVLDNDYKQFTQELQAIGTAGDFKYTLGFFYLNDDYDVYN
                     PRWNFQFGGNAFDISERGGGSKSYAGYGQLAWTPQALEEKLTASVGLRYTRESKHAYE
                     LLLSNSAYRANPAAPGSGVFE"
BASE COUNT         1143 a         2088 c         2154 g         1149 t
ORIGIN      
        1 cgacgcgcga ggcggccgcg cggcgcgagc cgagctatgc gcagcaatat gggctggtcg
       61 cgccggtcga tgtctatccg ctctacgaaa acgcgttgcg cgcttcgctg gggcagagcc
      121 tcgcggaagc gcaggacgaa agcggccgga tatggtcgct tttttcggag gttgccgccg
      181 ccaacccgca tgcgtggatt cgcaagggtg tcagcgcggc cgacgtgacg accgcttccg
      241 agagcaaccg gccgatcgcc tttccgtaca acaagctgat ggtcgccaac gcttcggtca
      301 atcagggggc cgccttcatc gtcaccagcg tggccgaggc gcgccggcgc gggatcgccg
      361 aggatcgatg gatttacatc ggcaatggag cggccgcgca cgagccgaac agcccgctcc
      421 agcgcgaccg ttacgaccag agcgcgagca tggcggtatc gatccgccgg acgctggcgc
      481 tgaatgcggt cgacccgggc gatctagacg tcgtcgagct ctatagctgc ttcccttgca
      541 ttcccaagct ggcgcggcgg ctgatcgact ggcccgtcga caggccggcg acggttttcg
      601 gcggactgac cttcggcggc ggaccgatcg gcaattacat gggccatgcg atcgcgagca
      661 tgaccgaccg gttgcgcgag acagggggga aggggctttt gttcgccaac ggcggctatg
      721 cgacgcacaa tcacagcatc gtgatcagca gcgcaccgat cccggccgcg tcctttcccc
      781 gcagctacga ctttcaagcg gaggctgatg caatgcgtga cgatattccg gcgctggcgc
      841 gcgaccatct cggtgacgcg accatcgaaa gctacacggt tctctaccgg cgcgacggct
      901 cgcccaaggc gggcgtcatc gtcgcccgga cacccgataa tgcgcggaca ctcgcccatg
      961 tgccgcccgg cgacgacata actctgcggc gactggtcga tcccgacgcc gatcccgtcg
     1021 gcgcggccgg gaggatcgtg gcgagcgaaa cgctacgcga atggcgcttc gcgtgatcgc
     1081 acggagcggt tttgagcgcg ccagaaggag gaaaggcggc ttttgctgtt caaagcggcg
     1141 cagggatggc atctattggc ctaacgccaa taggaaagga atgcttttgc cgtccagcca
     1201 agcgaagcga aggcaggggc cggggcggcc gctgaccccg ctgatcacgc gcgagatcgc
     1261 ggtccgcacg tcgatcgagc ttctcgatcg caagggtctc gacgggctct cggtccaggc
     1321 cgtggcgcgc gaactcaacg tccaggcgcc gtcgctctat tatcatttca agaaccgcga
     1381 cgaactgctt cacatgatcg cgaagacgtt gctggtcgag gtggggcggg gcgaaaagcc
     1441 gggctcgtca tgggaagagc gcgtcgtcga ccttgccgtt gcgacgcggc gcgtgatgct
     1501 gtgccacgcc catgccgggc cgctgatgct gcgcttcttt ccgcgcgaac tgatgcttgg
     1561 cgcgtatgaa cgcggtcttt cggactgtcc ctatgcgccg gaacaccacc tcgccattct
     1621 cgaggcgctc gaaaagctga cctatggcaa agcgcttttc gacgcggcgg cgaaggcgca
     1681 tcatcacgcc gcgatgccgc cggtggacgc cgatcgctat ccccgtctcg ccgaagcgct
     1741 ggccaagtcg acggtttcgg acgaggcctt gttcgtcgag acgctcgatg ttctgctcga
     1801 cggtttccgg tttcgttatg gcaaaaagga tcgaacgcag tgatcgaagg cgggtgcaat
     1861 tgcggcgcgg tgcgctatcg cgccgagggc gatccgatcg tggtggcgca gtgccattgc
     1921 accaattgcc gccggcagtc gggcagcgcc ttctcggtca acctgctctt caaaggggcc
     1981 gcggtcagcc atgaaggcga cctcgcggtt tatgaggaca aggacacttt ttccggcaat
     2041 gcggtgctgc gcaaattctg cggtcgctgc ggctcgccga tcttctcgga gcccaccgac
     2101 ggcaaggggc ttttgatcat caaggcggga acgctcgacg atcctgcgcc gttcgttccg
     2161 acggtcagcg tctggacctc gaccgcactg ccgtgggtga gcctgcccga gggccaatat
     2221 gcgttcgaga gaaacgcctg acctcgaaac gataaggcgc cggtcgcgtc agtccatgtc
     2281 ctccagcggg cgactgcctt tggcgaaggc cgacagcctg tgccgaattt cgcgggatcc
     2341 tccgaccagt tccccgaaac ggatcatgga ccgttcgatc gcgctgtcgt catcctcggc
     2401 ggcggcaagt tccatgatcg ccgcgacggc ggccggatgg cggcccgcaa atatggtcgc
     2461 caaatcgatc gcgcgtccga cgggatccgc ggtcaattcg tcgatcagcc ccaattcggc
     2521 ggccgccccg gcatttacga tcgcaccctc tagcgcgaaa cggcgcgcac ggtgcgcgcc
     2581 gatcagccgc gccaagcggg cgatcccgcc cgcgccgggg aaaatcccga gccggatttc
     2641 gggcaggccg atcgaataat tcggggagga ggccgcgatc cgcaaggtgc aggccagcgc
     2701 gatttcgaga ccgccgccca tgcagacgcc gttgatcgcc gcaatcacgg gcttttccga
     2761 cgcgtcgagc atatggccga tacgggcgaa aggcgaggcg gcgaaatctt cgggccgtat
     2821 cgccccgctc gcgacggcgt cgccggcgcg cctgatctgc gacacgtcgg cgtgccggat
     2881 gaacacgcct tcctcgccgc cggtcaagac caggcatcgc gtcgcttcgc gctccagttc
     2941 atggccgatc ctgtcggcga gcagcgccgc gcccttgttc gagataaggc ccgacggcgc
     3001 attggcatag cggatgacgg cgaccgcccc gtcgtggtcg accgtgaccg ttggcgcatc
     3061 gctcacgccg tatagccccc gtcgacgacg atctgcgtcc ccgtcacgaa gctgctctcg
     3121 tccgaaagca ggtacagcat cgctcccacg ctctcgtccg gctgcgccac ccgtccgagc
     3181 ggggtcagct gcgcgccgcg ttccagcgcc tggctattcg tcgtctcggt aaggaggcgc
     3241 gtggcgaaaa agccgggaat catcgtgttg acccggatgc cgtgcggcgc cagttccttc
     3301 gcggcggcgc gcgacatccc gatcactgcc gccttggcgg ccgaataggg gacgagcatc
     3361 gcatgcgcct tggttccggc gggcgaggcg acgttgacga tcgacccgcc gcccgtttcc
     3421 agcatcgcgc gtgcgccctc ccgcattccg aagaagacgc cggtctggtt gaccgcgatg
     3481 atgtccgccc atgcttcgtc gctgatctcg gtcagagcat gggtggcacc cacgcccgca
     3541 ttgttgacga gcccgtcgat ccggccgaac cggcttgtgg cgaagtcgat gaccgctttc
     3601 cagtcgctcg cgttggtgac gtcgagcggc cgatagtgcg cgccgccgcc aagatcggct
     3661 tcgatcgccg cccccgccgc tctatcccga ccgccgaaaa cgacgtgcgc gccctcgcga
     3721 accatcccgc ggacctgcgc ctcgcccatt ccgcgggtgc cgcccgaaag gacgataatt
     3781 tttttgtcta gccggcccgc catctatctc tccttttcgg cccggccggg ccgtcttctg
     3841 caggttttgc attatctata tactatagtt aaatgctctt ccatgcaatc tccggaaccg
     3901 cgatgtttcc aggatcgctt gtcgaaacac ggcttgccag agaggggtga gcaggacaga
     3961 aacttggcga aaaccggccg ggtgcggcct tcgggaggat ggaaatgagc aaggggttgt
     4021 ttgatcttac cggaaaggtc gcactggtga ccggcggcaa tggcgggatc gggctcggca
     4081 tggcaaaggg tcttgcgatg cacggcgcga gcgtcgtgat ctggggcaac aaggccgaaa
     4141 agaacgagcg cgccgtcgcc gagctcgagg cgttcggcgg tgcggttcgc tgcgcgcagg
     4201 tcgacgtcgc cgacgaggcg gcggtgaagg cgggcatcgc cgcgatcgtc gaggagttcg
     4261 gccgaatcga tcaggcgatc gccaatgccg gcatcggcat cccccgccgt gacatgttcg
     4321 agatcagcca agccgatttc gaaaagctgc agtccgtcaa cgtatatggg gttttctaca
     4381 cgctgcggga ggcggccgcc catatgatcg aacgcggcaa atcgggcgat cgcggcggat
     4441 cgctggtgtc gatcgcctcg acggcatcga ttcacggcgc cgcccgcaac gaacattatg
     4501 gcgcgaccaa aggcgcggtc ctgtcgctca gccgcgcgat ggccgtcgag ctcgcgccgc
     4561 atgccatccg cgtaaacagc attctgcctg gctggacgcg gtccgacatg accgagcgcc
     4621 tgcaaggttg ggacaagttc aacgagaatg tgatcagccg ggcgcccgtc ggtcgctggg
     4681 gcgacggcga ggatttctcc gccattgccg tctatcttgc gagcaagcac tccagctttc
     4741 accttggcga tgcgatcgtg attgacggag gatatacgat ctattgagcc gtttgccgac
     4801 cggctcggcg tgatcgatcg cgtcgttgct tcgtgattcc ggctttcagg ttccgttttg
     4861 cgatcctatc ggaatggcgc catttcccgt tcgaccggcg cgttcaggcg cctgaaaaat
     4921 cccgcgccgg gaggcgaaat gctttgtgcc ttgttgcggc cgccgggctg cttattcgcc
     4981 acaagggtga acagtgtagt tacgatggcg cgctttatcg ccatggaggg tcggttatcg
     5041 tcccgctccg taatcgggcc aaacaggaag aagcccggat atgggcggcc acaaagagcc
     5101 gcggttggag agggaggaat tatggcattc cgcaaagctg ccctttgggc ggtcatggcc
     5161 gctacggcgc tcaattcatc ggtcgtgatg gcgcaggagg catccgaagg cgaagcggcg
     5221 gacagtgccg cgcccgtcga gggcgatatc gtcgtcaccg cgcagcgccg cgaggagcgg
     5281 ctcgtcgacg tgccgatttc ggtctccgcc gtcagcagcg ccgcgatcga ccgcgcgcag
     5341 gtccgcaccg tgagcgacat cgcgacggtc gtcccgaaca tccagatcaa cgaaacgatc
     5401 ggcaacagct atggtccgct gatcacgatc cgcggcctgt cgccgtcggc cgacaccagc
     5461 cttgcccgcg accagccggt ggggctctat atcgacggcg tcccgatcgg gaagtcgacc
     5521 ggcgccgcct tcgacaccgt cgatctggag cgcgtcgagg tgctgcgcgg gccgcagggc
     5581 acgctttacg gcaagaacac gatcggcggc gcggtcaacc tgatcacgcg caagccgagc
     5641 ggcgagttcg gcgggcaatt tctggcgggc gccggcagct gggggcagtt caaccagcgg
     5701 atatcggtcg acctgcccga ggtcgcgggg ttcagcgcca aggtcggcct gatcaccaag
     5761 ctcgacggcg gctattaccg caattcggcg acggggaaga atttcggcga ggaagaatta
     5821 tgggcggcgc gcggcgacct cttgtggcgt ccgtcggaca gcttcagcgc gctctacagc
     5881 tatgatattt cggacagcaa gggcacgccg aaccagctcg ccgcgaccgc ggtcaccgga
     5941 tcgggcgcga ccgccttcgt caacgcgctg atggcgccct atgtcgtcga cgggcggacg
     6001 cgcgagatcg gggcgcagag cgcgcttcgc agcgactatc gcaccaacgg gcacgcgctg
     6061 acgctcgaat gggagccggg gctcggcgat ctgacgctga agtcgatcac cgcgcggcgc
     6121 accgcgataa cgcggtcggc gagcgacttc gacggcagcc cgctcgacct tttgcgcatc
     6181 gtcctcgaca atgattacaa gcagttcacg caggaattgc aggcgatcgg gaccgcgggc
     6241 gatttcaaat acacgctcgg cttcttctat ctgaacgacg attatgacgt ctataatccg
     6301 cgttggaact tccagttcgg gggcaacgcc ttcgatatca gcgagcgcgg gggcgggtcc
     6361 aaatcctacg ccggctatgg ccagctcgcc tggacgccgc aggcgctcga agagaagctg
     6421 accgcgtcgg tcgggctgcg ctacacgcgc gaatcgaagc atgcctacga gctgctcttg
     6481 tccaattcgg cctatcgcgc caatccggcg gcgcccggat cgggggtgtt cgag
//
LOCUS       DATMSU010000229         6914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_136503,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000229 DATMSU010000000
VERSION     DATMSU010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6914
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136503"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..729
                     /locus_tag="VN034_05810"
     CDS             82..729
                     /locus_tag="VN034_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWV60146.1"
                     /translation="MTALIALFWRDLRRAWGSGALWLPVLFFLLVATAFPFAVGPDAP
                     LLRRAGGGMVWVAALLAALLPIDRLVRPDKDAGVLDQLAVRGFADEVIAAVKIGAHAI
                     GFGLPLLIALLPASALLAQDGARVETLAIGIAIAVPALASLAVLSAALTANHKGGSAI
                     GGLLVLPLAVPLLIFGAGMLDPAGRGAIKLLAATSLLLTVIGPFAAGAALRGLRE"
     gene            726..1145
                     /locus_tag="VN034_05815"
     CDS             726..1145
                     /locus_tag="VN034_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV60147.1"
                     /translation="MTRQSLVHVALVVRDYDEAIDFYVGTLGFTLVADDYQPEQDKRW
                     VLVAPPGSPPGHATILLARAANAEQAEFIGNQSGGRVFLFLETDDFARDYQRLLDKDV
                     RIEREPMEADYGTVAVFLDLYGNKWDLIEFRKSAVQT"
     gene            complement(1197..1895)
                     /gene="rplA"
                     /locus_tag="VN034_05820"
     CDS             complement(1197..1895)
                     /gene="rplA"
                     /locus_tag="VN034_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HWV60148.1"
                     /translation="MAKLTKKQKALEGKVDALKLHGVDEAIKLVRDLATAKFDETLEI
                     AMNLGVDPRHADQMVRGVVTLPAGTGKDVKVAVFARGDNAEKALAAGADKVGAEDLME
                     DMLAGNLDYGRVIATPDMMGIVGRLGKTLGPKGLMPNPKLGTVTPNVAEAVKAAKGGQ
                     IEFRVEKVGIIHAGLGKLSFGEEKLRQNFDAFVDAIVKAKPSGAKGKYVRKVALSSSM
                     GPGVKVDTADVAGA"
     gene            complement(1900..2331)
                     /gene="rplK"
                     /locus_tag="VN034_05825"
     CDS             complement(1900..2331)
                     /gene="rplK"
                     /locus_tag="VN034_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HWV60149.1"
                     /translation="MAKKISGYIKLQVPAGTANPSPPLGPALGQRGVNIMEFCKAFNA
                     ATDSLDKGTPTPTIITVYADKSFSFVTKTPPATYLIKKAANLKSGSKEPGKISGGKIA
                     RSKLAEIAEIKMKDLNANDIEQATKIIEGSARSMGLEVTEG"
     gene            complement(2487..3413)
                     /locus_tag="VN034_05830"
     CDS             complement(2487..3413)
                     /locus_tag="VN034_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HWV60150.1"
                     /translation="MSLTPEELQARLDLAREINSSTPADKVVGAVARLFDLDAVPGVD
                     DEFTFPARPTTSRDRIFGGQVIAQAMTAAARTVDPDKQVHSLHAYFLRGGDETKPLHF
                     RLHRDFDGRSFSNRRVVVRQDGKVIFNLTASFQIPAEGLSHQVPMPDLLAPEECWDFT
                     DFIAADPHISDRQLAHMARSRPFEVRSFRPPASDRATQHYQWFRVASPIGDDPLLHRG
                     FLAFASDMGLLSSAMLPHGVSFWTPGLVSTSLDHAMWFHGDVRVDDWFVFVMDSPWTG
                     GDRGLCRGSIYRRDGTLMATVAQEGLIRYRPQ"
     gene            complement(3465..4001)
                     /gene="nusG"
                     /locus_tag="VN034_05835"
     CDS             complement(3465..4001)
                     /gene="nusG"
                     /locus_tag="VN034_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HWV60151.1"
                     /translation="MARWYIIHAYSGFENKVRDSVLSEAERMGLTDFVEAVEVPVETV
                     TEVKRGKKVQAERKFFPGYVLAKLTMTDDVYHLVKNTPKVTGFLGSSGKPQAISEAEA
                     ARILNSKEEAAARPKTEIRVDYEIGDQVKVLDGPFASFNGLVEELDFEKARVKVSVSI
                     FGRATPVELDFEQVERFK"
     gene            complement(4036..4233)
                     /gene="secE"
                     /locus_tag="VN034_05840"
     CDS             complement(4036..4233)
                     /gene="secE"
                     /locus_tag="VN034_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HWV60152.1"
                     /translation="MAKTSPAEFINQVRAEARKIVWPTSRETVQTTIMVLIMTTILSL
                     FFLGVDTVFNAIVSWLTSLAR"
     gene            complement(4392..5738)
                     /locus_tag="VN034_05845"
     CDS             complement(4392..5738)
                     /locus_tag="VN034_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60153.1"
                     /translation="MASTALPSAAPVTETPALRPRTVAAYAATAGPTVMLGLPFSVYL
                     PPYIAEGGAISVALVGLLFSLTTIWDGVVDPLIGTMVDRVRTGLGAHRRWMLLALVPL
                     SLLLLALVTVGNQLPFAALFLMMVLFYSSYSLYEVAQLSWGSALVRSPADSALLYGMR
                     DWFAKIALILAFAAPAAAQLLIPGLSLQGRIMAYASLFLLLVPIALFAIRRVPPRTVV
                     AESGFGWRHEIRASLSFRPLMLLLGVQFLNSFAFGSLTSLFVFFAAAVLDLDAQSAVL
                     LFASFIGGALASPIWTFLARRFGKPPMMMAMGLLIGSLLVATLAQEPRGLGQAVGFSL
                     MLGTGFVGLLFTYSMLADLIPRDSERCGRNRSAFLFALLNLMAKFGVAAAIAVSYAVL
                     DLVGFDPQNGQAAARELHLIFALQPTAAWIMMVGLLLWMQRELTRAPALAIAAPPK"
     gene            complement(5819..6424)
                     /locus_tag="VN034_05850"
     CDS             complement(5819..6424)
                     /locus_tag="VN034_05850"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV60154.1"
                     /translation="MDTPSPRERRQGAILDAAESLFLEQGYERTSLAEIVKRSGGSLA
                     TLYELFGNKQGLLRAIATRWCDEAMLRSLDEDAIRGLSSVEILKRYAHRQKELMESPH
                     AVALMRMLISESLRDHEFAMQIYLDLHVPALEELIELFAEWAATGKAEIDRPDAAARL
                     FFDIVVGDSMLNTLMGIEVEWLDSEQIDWRLQPFLSHFKIR"
     gene            6567..>6914
                     /locus_tag="VN034_05855"
     CDS             6567..>6914
                     /locus_tag="VN034_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWV60155.1"
                     /translation="MSRVRPLSCAAGAALAFLLAACSSEAPPAPPPPEVNIVTIRTQA
                     VPNVIELPGRVQAYRTSEVRARVNGIVERRLFNEGSYVRAGTPLFRIDPRELIATANA
                     ARAQLARAQATAAN"
BASE COUNT         1219 a         2220 c         2257 g         1218 t
ORIGIN      
        1 gaacggcggc gcggtgatgc tggcgtcgca tttcgcgctc gggatccccg atctggccga
       61 actcgaaatg ggggcactcg cgtgaccgcg ctcatcgccc tcttctggcg agatctgcgc
      121 cgcgcgtggg ggagcggggc gctgtggctg ccggtgctgt ttttcctgct cgtggcgacg
      181 gcctttccgt tcgcggtcgg gcccgatgcg ccgttgctgc ggcgcgcagg cggcgggatg
      241 gtgtgggtcg cggctttgct tgccgccctg ctcccgatcg accggctggt gcggccggac
      301 aaggatgccg gcgttctcga ccagctcgcg gtgcgcggct tcgccgacga ggtcatcgcc
      361 gcggtgaaga tcggtgcaca tgccattggt ttcgggctac ctttgcttat cgcgcttctt
      421 cctgcatcgg cgctgctcgc gcaggacggg gcacgcgtcg agacgctcgc gattggcatc
      481 gcgatcgccg tccccgcgct tgcttcgctc gcggtgctga gcgcagcgct gaccgcgaac
      541 cacaagggtg gcagcgcgat cggcggattg ctcgtcctgc cgctggccgt gccgttgctg
      601 atcttcggtg ccgggatgct cgacccggcg gggcgcggcg cgataaaatt gctcgcggcg
      661 acgagcctgc tcctgaccgt gatcgggccg ttcgcggccg gcgcggccct ccgaggcctc
      721 cgcgaatgac gcgccagtcg ctcgtgcacg tcgcgctcgt ggtgcgcgat tatgacgagg
      781 cgatcgactt ctatgtcggc acgctcggtt tcacgctcgt cgccgacgat tatcagcccg
      841 agcaggataa gcgttgggtg ctggtggcac cgccaggaag tccaccgggg cacgcaacga
      901 tcctgcttgc ccgcgcggcc aatgccgaac aagcggagtt catcggcaat cagtcgggcg
      961 gccgcgtctt tctatttctc gaaaccgacg atttcgcgcg cgactatcag cgcctgctcg
     1021 acaaggacgt gcggatcgag cgcgagccga tggaagccga ttatggcacc gtcgcggtgt
     1081 tcctcgacct ctatggcaac aagtgggact tgatcgaatt tcgcaagtcc gccgttcaga
     1141 cataagaaaa gggccggagt ttcccccggc ccttccctaa ttcgttgcgg tgccgatcag
     1201 gcgccggcga cgtcggcggt atcgaccttc acgcccgggc ccatcgacga cgacagcgcg
     1261 accttgcgaa catacttgcc cttggcgccc gacggcttcg ccttgacgat cgcgtcgaca
     1321 aaggcgtcga agttctggcg gagcttttcc tcgccgaacg acagcttgcc gaggccggcg
     1381 tggatgatgc cgaccttttc gacgcggaat tcgatctgac cgcccttggc ggccttcacg
     1441 gcttcggcga cgttcggggt gacggtgccg agcttcgggt tcggcatcag gcccttcgga
     1501 cccagcgtct tgccgaggcg gccgacgatg cccatcatgt ccggcgtcgc gatgacgcgg
     1561 ccatagtcga ggttgcccgc gagcatgtct tccatcaggt cttcggcacc caccttgtcg
     1621 gcgccggcgg ccagcgcctt ctcggcatta tcgccgcgcg cgaacacggc gaccttgacg
     1681 tccttgccgg tgccggcggg gagcgtcact acgccgcgga ccatctggtc ggcgtggcgc
     1741 ggatcgacgc cgaggttcat cgcgatttcc agcgtttcgt cgaacttggc ggtcgcgagg
     1801 tcacgcacga gcttgatcgc ttcatcgacg ccgtggagct tcagtgcgtc gaccttgccc
     1861 tcgagggcct tctgcttctt ggtcagcttt gccatgggtt cagccctccg tcacttcgag
     1921 gcccatcgag cgggcgctgc cttcgatgat cttcgtcgcc tgttcgatgt cgttggcgtt
     1981 gagatccttc atcttgatct cggcgatttc ggcgagcttc gagcgcgcga tcttgccgcc
     2041 gctgatcttg ccgggctcct tcgaacccga cttcaggttc gcggccttct tgatcaggta
     2101 ggtcgccggg ggcgtcttgg tgacgaaaga gaagctcttg tcggcataga cggtgatgat
     2161 ggtcggggtc ggggtgccct tgtccaggga atcggtcgcg gcgttgaacg ccttgcagaa
     2221 ttccatgatg ttgacgccgc gctgaccgag cgccggcccg agcggcggcg acgggttggc
     2281 ggtgccggcg ggcacctgca gcttgatgta gccgctgatc ttcttggcca tgacggcctc
     2341 ctttctttct gtcgccccgg acctggatcc ggggctcaaa ataagcggtc gaacagaagg
     2401 gcatcacccc ttcctcccgc gcggaatcgc aaccttgtct gatgcgaagc ggcgcccata
     2461 aggccaaagg cgccgaaaaa caagccttat tgggggcggt agcggatcag gccttcctgc
     2521 gcgacggtcg ccatcaatgt gccgtcgcgg cggtagatcg agccgcggca cagcccgcgg
     2581 tcgccgcccg tccacgggct gtccatcacg aagacgaacc agtcgtcgac gcgcacgtcg
     2641 ccatgaaacc acatcgcatg atcgaggctg gtcgacacga ggcccggggt ccagaagctg
     2701 acgccgtgcg gcagcatcgc cgacgacaaa agccccatgt cggacgcgaa agcgaggaaa
     2761 ccgcggtgga gcaacggatc gtcgccgatc ggcgatgcga cgcgaaacca ttgataatgc
     2821 tgcgtcgcgc ggtccgacgc cggcggccgg aagctgcgca cctcgaacgg gcggctgcgc
     2881 gccatatgcg cgagctggcg gtccgaaata tgcgggtcgg ccgcgatgaa atcggtaaaa
     2941 tcccagcatt cctccggggc cagcaggtcg ggcatcggaa cctggtgcga caacccctcg
     3001 gccgggatct ggaacgaggc ggtcaggttg aagatcacct tgccgtcctg ccgcacgacg
     3061 acacgacggt tcgagaagct gcggccgtcg aaatcgcggt ggaggcggaa gtgcagcggc
     3121 tttgtctcgt cgccgccgcg caggaaatag gcgtggagcg aatgcacctg cttgtcggga
     3181 tcgaccgtgc gcgcggccgc agtcatcgcc tgcgcgatca cctgcccacc gaaaatccgg
     3241 tcgcgcgagg tcgtcggccg cgcgggaaag gtgaattcat cgtcgacgcc gggaacggcg
     3301 tcgaggtcga acagacgggc gacggcgccg accaccttgt cggcgggcgt gctgctgttg
     3361 atttcacgcg caagatcgag gcgcgcctgc agctcttcgg gcgtgaggct catgcgcaat
     3421 tcccccgggc tgggaccacc cgcgcccgcg tgggcgcagc gcggttactt gaagcgttcg
     3481 acctgttcga agtcgagttc gaccggggtg gcgcgaccga agatcgaaac cgacaccttg
     3541 acgcgcgcct tttcgaaatc gagctcctcg accagcccgt tgaagctggc gaagggaccg
     3601 tcgagcacct tgacctgatc gccgatttca taatcgacgc ggatttcggt cttcggacgc
     3661 gccgcggcct cttccttgct gttgaggatg cgcgcggctt cggcttcgct gatcgcctgc
     3721 ggcttgcccg acgaaccgag gaagccggta accttcggcg tgttcttgac gaggtgatag
     3781 acatcgtcgg tcatcgtcag cttggcgaga acatagccgg ggaagaattt gcgttcggcc
     3841 tgaaccttct tgccgcgctt gacctcggtg acggtttcga ccggcacctc gacagcttcg
     3901 acgaagtcgg tgagacccat gcgttcggct tcggaaagca ccgaatcgcg aaccttgttc
     3961 tcgaaacccg aataggcgtg aatgatgtac cagcgcgcca tgtctatccg tatcctgtcc
     4021 ctgtaaccgc gggcgttagc gcgcgagcga cgtcagccag ctgacgatcg cgttgaaaac
     4081 cgtgtcgacg ccaaggaaga agagcgaaag gatcgtcgtc atgatcagca ccatgatcgt
     4141 cgtctgcacc gtttcgcggc tcgtcggcca aacgatcttg cgagcttcgg cgcgcacctg
     4201 attgatgaac tcagccgggc tggtcttggc catgtcgatc gtcctgtcct tgcaacgtca
     4261 atgatccggg ccagatgggt tgccgccgcc cgcgcgtcaa gcgcgctttg ggcaagtgtc
     4321 cgcccaccct ccccgagcat gtcggcgaaa ggcggaaagg gaacggcgca tctgtccgaa
     4381 tgaagcggcc tttacttggg cggcgccgcg attgcaagcg cgggcgcacg ggtgagttcg
     4441 cgctgcatcc agagcaagag gccgaccatc ataatccacg cggcggtcgg ttgcagcgcg
     4501 aagatcagat gcagctcgcg cgccgccgcc tggccgttct gcggatcgaa accgacgagg
     4561 tcgagcaccg catagctgac cgcgatcgcg gcggcgacgc cgaacttcgc catcaggttc
     4621 aacagcgcga agagaaaggc cgaccgattg cgcccgcacc gctcgctatc gcgcggaatc
     4681 aggtcggcga gcatcgaata ggtgaagagc agcccgacga agccggtgcc gagcatcagc
     4741 gagaagccga ccgcctgccc cagcccgcgc ggttcctgcg ccagagtcgc gacgagcagg
     4801 ctgccgatca gcagccccat cgccatcatc atcggcggtt tgccgaagcg gcgcgcgagg
     4861 aaggtccaga tcggcgacgc cagcgccccg ccgatgaagc tcgcgaacag cagaacggcg
     4921 ctctgcgcat cgaggtcgag cacggcggcg gcgaagaaca cgaagagcga ggtcagcgac
     4981 ccgaaagcga agctgttgag gaactgcacc ccgagcagca gcatcaacgg ccggaaggac
     5041 agcgaggcgc ggatttcgtg tcgccatcca aagccggatt cggccaccac cgtgcgcggc
     5101 ggcacgcggc ggatcgcaaa gagcgcgatc ggcaccagca ggaggaagag gctggcatag
     5161 gccatgatcc gcccttgcag gctgagcccg gggatcagca gctgcgccgc ggcaggcgcg
     5221 gcgaaagcga ggatcagcgc gattttcgca aaccagtcgc gcatgccata gagaagcgcg
     5281 ctgtcggccg gcgaacggac aagcgccgag ccccacgaga gctgcgccac ctcgtagaga
     5341 ctgtagctcg aataaaagag caccatcatc agaaagagcg ccgcgaaggg cagctgattg
     5401 ccgacggtca cgagcgcgag cagcagcagc gacaagggaa cgagcgcaag cagcatccag
     5461 cgccggtgcg cgccgagccc ggtgcgcacg cggtcgacca tcgtgccgat cagcggatcg
     5521 acgacgccgt cccagatcgt cgtgagcgaa aagagcagcc cgaccagcgc gaccgaaatc
     5581 gccccgccct cggcgatata gggcggcaga tagacgctga acggcaggcc gagcatgacc
     5641 gtcggccccg cggtcgcggc ataggcggcg acggtgcgcg gacgcagggc gggtgtttcc
     5701 gtgaccgggg cggccgatgg cagggcggta ctggccaaga tgcgaagctc ccgaaacgga
     5761 agccgcaagc tttcagctat taacaatcat gtcaacacca acgcgtggtg cagggcccct
     5821 accgaatctt gaaatgcgaa aggaacggct ggagccgcca gtcgatctgt tcgctgtcga
     5881 gccattcgac ttcgatcccc atcaaggtgt tcagcatcga atcgccgacg acgatatcaa
     5941 agaacaggcg cgcggcggcg tcggggcggt cgatctcggc cttgcccgtc gccgcccatt
     6001 cggcgaacag ctcgatcagt tcctccagcg cgggcacatg aaggtcgagg taaatctgca
     6061 tcgcgaattc gtggtcgcgc aaactttcgg atatcaacat gcgcatcagg gcgacggcgt
     6121 gcggcgattc catcagctct ttttgacgat gcgcatagcg tttcaggatt tcgacgctcg
     6181 acagcccccg gatcgcatcc tcgtcgagcg acctgagcat ggcttcgtcg caccagcgcg
     6241 tcgcgatcgc gcgaagcagc ccctgcttgt tgccgaacag ctcgtaaagc gtcgcgagcg
     6301 aaccgcccga acgtttgacg atttcggcaa ggctggtgcg ttcatagccc tgctccagaa
     6361 atagcgactc ggccgcatcg agaatggcgc cctgtcgcct ctcgcgcggc gacggcgtat
     6421 ccatttgaca taaatcggat tccagcctgg tcaatttgcg gcccctcttc ccttgctctt
     6481 ttttgtaatt taaattacac ggcccctcag cgcaagcttt tgcgtatata acaatcccgc
     6541 acaactgcga tttttcaggg gttcatatga gtcgcgtccg tcctctcagc tgcgccgccg
     6601 gcgctgcgct ggcctttctg ctggccgcct gctcgtcgga agcgcccccc gcgcccccgc
     6661 cgcccgaggt caatatcgtg acgatccgca cacaagcggt gccgaacgtc atcgaactcc
     6721 ccggccgggt acaggcgtat cgcacgtcgg aagtccgcgc gcgcgtcaac ggcatcgtcg
     6781 agcggcggct gttcaacgag ggcagctatg tgcgcgccgg aacgcccctg ttccggatcg
     6841 atccgcgcga gttgatcgcg accgccaatg cggcgcgcgc ccagcttgcc cgcgcgcagg
     6901 cgaccgccgc caac
//
LOCUS       DATMSU010000230         3528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_89585, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000230 DATMSU010000000
VERSION     DATMSU010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89585"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VN034_05860"
     CDS             <1..352
                     /locus_tag="VN034_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60156.1"
                     /translation="PADAAPSRKKRGRWGGCLPWAIVGAVLFAIFAIWKFPSFKAQAE
                     LGSAYAARVGCSCRYVQSRSLESCQSDFEPGMEMVSLSEDPATKTVTGSVPLLASRSA
                     RYAGASGCLMRPDN"
     gene            complement(413..1336)
                     /locus_tag="VN034_05865"
     CDS             complement(413..1336)
                     /locus_tag="VN034_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV60157.1"
                     /translation="MIGDFEQQRVKLSTGVELEVVDMGPKDAPALIFLHGFPESHRTW
                     RHQLPHFADRFRCIAPDQRGYRGSSKPQEVEAYTPDKLIADIFALADALEVDKFTIVG
                     HDWGGAIAWGVALAGQPGGLHPAWAGRVTRAVIANAPHPGIFQRLLATNEAQRAASQY
                     IRTFRDPASDKILEDHGIAGILAHAFADRVPSGGLQPADEIGRLLGDWEDRDACRAMI
                     NWYRASTIEVPAMDEPYAEPPAVPFPKLAVPTLVIWALDDVALPPCNLDGMGDLVPGV
                     SIVEVSGCGHFVPWEAPDTVNRAMDAFLAQS"
     gene            complement(1697..2581)
                     /locus_tag="VN034_05870"
     CDS             complement(1697..2581)
                     /locus_tag="VN034_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWV60158.1"
                     /translation="MFTVITPSTHDIGAFDVRRTLPNKARTMVGPFIFVDQFGPAHFD
                     IGQGMDVRPHPHINLSTLTYLFEGAIDHRDSLGTFATIRPGACNLMTAGRGIVHSERT
                     PQAERATGSGISGMQTWLALPDGKEEIDPAFEHIAAGDLPLVEDGHVSARVIMGSLWG
                     ATAPTTQHSATIYADIMMSAGATLPIDTEADERAVLVALGDASLDGEVLERYSLYILK
                     PGQAMTLRAESDARVMLLGGEAFTTPRHVWWNFVSSSRDRINEAKQEWKERKFPLVPG
                     DSEEFIPIPEVPKTVSYP"
     gene            complement(2581..2949)
                     /locus_tag="VN034_05875"
     CDS             complement(2581..2949)
                     /locus_tag="VN034_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV60159.1"
                     /translation="MPDKVNLADAFAKIPDAWNPRVAGDINDFQIKLVKLDGKFDWHH
                     HDIEDELFLVVAGRMKMAFRDRDVIVEPGEFIIVPHGVEHCPEALDGECHVVLLEPGT
                     TRNTGNVETEKTRKTLERLG"
     gene            complement(2952..3230)
                     /locus_tag="VN034_05880"
     CDS             complement(2952..3230)
                     /locus_tag="VN034_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWV60160.1"
                     /translation="MSNKGPVQQEMESLLAAAFPDANFTLSNDSASHHGHMGDDGSGE
                     SHFSLAIEWAGFAGMNRVARQRTVNKALGDLPGQRVHALAIKAVAPGE"
     gene            3312..>3528
                     /locus_tag="VN034_05885"
     CDS             3312..>3528
                     /locus_tag="VN034_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWV60161.1"
                     /translation="MPSSARPSRFHGRVPREERCAAPGCAEAGEFRAPVSSHRSPDGP
                     PPYRWLCLDHVREFNAGYNFFEGMNADQ"
BASE COUNT          579 a         1156 c         1158 g          635 t
ORIGIN      
        1 gcccgccgat gcggcgccgt cgcggaagaa acggggccgc tggggcggct gcctgccgtg
       61 ggcgatcgtc ggcgcggtac tgttcgccat cttcgcgatc tggaaattcc cgtcgttcaa
      121 agcgcaggcc gagctggggt cggcctatgc cgcgcgcgtc ggctgttcgt gccgctatgt
      181 ccagagccgc agcctcgaaa gctgccagag cgatttcgag cccgggatgg agatggtgtc
      241 gctgagcgag gatcccgcca ccaagacggt gacgggcagc gtcccgttgc tcgcgtcgcg
      301 cagcgcacgc tacgcgggcg cgagcggttg cctgatgcgg ccggataact agaatcccgc
      361 tcctattcct gcccgtttgc cggcaaatcc atgatcaaca gaagctgtgc catcaacttt
      421 gggcgaggaa cgcatccatc gcgcgattga ccgtgtcggg cgcctcccac ggcacgaaat
      481 gaccgcagcc cgaaacctcg acgatcgaca cgccggggac gaggtcgccc attccgtcga
      541 gattgcacgg cggtagcgcg acatcgtcga gcgcccagat caccagcgtc gggacggcga
      601 gtttgggaaa gggcaccgcg ggcggttcgg catagggttc gtccatcgcg ggcacctcga
      661 tcgtcgaggc gcgataccag ttgatcatcg cgcggcacgc gtcgcgatcc tcccaatccc
      721 ccagcaagcg gccgatctcg tcggcgggct ggaggccccc gctcggcacg cgatccgcaa
      781 aggcatgggc gaggatgccc gcaatgccat ggtcctcgag aatcttgtcg ctcgcggggt
      841 cgcgaaaggt gcggatatac tggctggccg cgcgctgcgc ttcgttcgtc gcgagcaggc
      901 gctgaaagat gccggggtgc ggcgcgttgg cgatcaccgc ccgcgtcacg cgccccgccc
      961 atgccgggtg gagcccgccc ggctggccgg cgagcgcaac accccaggcg atcgcgccgc
     1021 cccagtcgtg cccgacgatg gtgaatttat cgacctccag cgcatcggcg agcgcgaaaa
     1081 tgtcggcgat cagcttgtcg ggcgtgtagg cttcgacctc ctgcggcttc gacgaaccgc
     1141 gatagccgcg ctggtcgggg gcgatgcagc ggaagcggtc cgcgaaatgg ggcagttggt
     1201 gacgccaggt gcggtgcgat tcgggaaagc cgtggagaaa gatcagcgcc ggggcatcct
     1261 tcggccccat gtcgacgact tcgagctcga cccccgtcga aagcttcacc cgctgctgtt
     1321 cgaaatcgcc gatcattcgc cctgcctcct ttggtttcgt ttcgaaacca atctgccgag
     1381 gattggtgtg gctggcaagc tgctacagat gaggggtcac ccgaaaggcg acggagatcg
     1441 ctgcaccgtc tgtcttgggg gtggggagcg gacgttgccc taaacccctc ctcttcagag
     1501 gaggggcagt gagacttggt ccggcgcagc cgggccttta gtcgaacggg gtggtggggg
     1561 cggcacgcgc gcgcctggcc acgacgacca ccccgctgcg actaacgaac aagttcgtaa
     1621 gtctcgctac ccctcctctg aagaggaggg gctgacgcat atttgccaac gtcagctacc
     1681 ggtcggttgc cgacgttcac ggataactca ccgtcttcgg cacttcgggg atcgggatga
     1741 actcttcgct atcgccgggg acgagcggga atttgcgttc cttccactcc tgcttcgcct
     1801 cgttgatgcg gtcgcgcgac gagctgacga aattccacca cacatggcgc ggcgtcgtga
     1861 acgcctcgcc gccgagcagc attacgcgcg catcgctctc cgcgcgtagc gtcatcgcct
     1921 gcccgggctt gaggatatag aggctgtagc gttcgaggac ctcgccatcg aggctcgcat
     1981 cgccgagcgc caccagcacc gcgcgttcgt cggcctcggt atcgatcggc aaggtcgcgc
     2041 cggcactcat catgatgtcg gcataaatgg tcgcgctgtg ctgcgtcgtc ggcgcggtcg
     2101 cgccccacag gctgcccatg atcacgcgcg ccgacacatg accgtcctcg accagcggca
     2161 ggtcgccggc ggcgatatgc tcgaacgccg ggtcgatctc ttccttgccg tcgggcagcg
     2221 cgagccaggt ctgcatgccc gaaatgcccg aacccgtcgc gcgctcggcc tgcggcgtgc
     2281 gctccgaatg gacgatgccg cggcccgcgg tcatcaaatt gcacgcgccg gggcggatcg
     2341 tcgcgaacgt gccgaggctg tcgcgatggt cgatcgcgcc ctcgaacaaa taggtcaggg
     2401 tcgacaggtt gatatgcggg tgcggccgca cgtccatccc ctgcccgatg tcgaaatgcg
     2461 ccgggccgaa ctggtcgacg aagatgaagg gaccgaccat cgtccgcgcc ttgttgggca
     2521 gcgtacgccg cacgtcaaag gcgccgatgt cgtgggtcga aggggtgatg acggtgaaca
     2581 tcaaccgagc ctttccagcg tcttgcgcgt cttttcggtt tcgacattgc cggtgttgcg
     2641 cgtcgtgccg ggttcgagca ggacgacatg gcattcgccg tccagcgcct cggggcaatg
     2701 ttcgacgccg tgcggcacga tgatgaactc gccgggctcg acgatcacgt cgcggtcgcg
     2761 aaacgccatc ttcatccggc cggcgacgac gaggaatagc tcgtcctcga tatcatggtg
     2821 atgccagtcg aacttgccgt cgagcttgac cagcttgatc tgaaaatcat tgatgtcgcc
     2881 ggcgacgcgc gggttccatg cgtcggggat tttggcgaag gcatcggcga ggttgacctt
     2941 gtcgggcatc ctcattcccc cggcgcaacg gccttgatcg cgagcgcatg gacgcgctgt
     3001 cccggcaggt cgccgagcgc cttgttcacc gtgcgctggc gcgcgacgcg attcattccg
     3061 gcgaagcccg cccattcgat ggcgaggctg aaatgcgact cgccgctgcc atcgtcgccc
     3121 atatggccgt gatggctggc gctgtcgttg ctgagggtga aattcgcgtc gggaaaggct
     3181 gcggccagca ggctttccat ctcttgctgc acggggcctt tgttgctcat gggcttgacc
     3241 ataggccctc catcgccaaa ttataaggcc ccccggagac caacgtccca ccccgattcc
     3301 gaaagcccgt tttgccgtct tccgcccgcc cttcccgatt ccatggccgc gtcccgcgcg
     3361 aggaacgctg cgccgcgccc ggatgcgccg aggccggcga gtttcgcgcc cccgtctcgt
     3421 cgcaccgctc gcccgacggg ccgccacctt accgctggct ctgcctcgac catgtgcgcg
     3481 aattcaacgc gggctataat ttcttcgaag ggatgaacgc cgaccaga
//
LOCUS       DATMSU010000231         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_79715, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000231 DATMSU010000000
VERSION     DATMSU010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79715"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VN034_05890"
     CDS             <1..628
                     /locus_tag="VN034_05890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60162.1"
                     /translation="AIEALGRKAKAYQASVTDEAACAAMVAAVEADFGPMSILVNNAG
                     IASRGLSVADTDPTEVDKLLAVHAAGPHRLSRLALPQLRQHKRSDIIVISSIATYTHS
                     PNGAPYTMAKAAGEALAYALAKEELGNGVRVNIVAPALTVSDMGEKLSRAITGNTDIH
                     HLDTKMPFGRVAVPADVAAAVVWFVSDANPYCSGQKLNIDGAGQATFR"
     gene            625..2226
                     /locus_tag="VN034_05895"
     CDS             625..2226
                     /locus_tag="VN034_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HWV60163.1"
                     /translation="MKHMLFVATFALALGGCATAQSKEARAAAETPAPDAPMFAPTEV
                     ESRGSVTVGGVPIPYRAVAGTLVVHPQGWDDTVPIERKRDKDAAADVPNSEASMFYAA
                     YFKEGAPAESRPITFLFNGGPGSPTLWLHMGAYGPVRIETPDLTHGSAPYRTVANDQS
                     LLDVSDLVFVDAPGTGFSRVAGKDKDKAWFGVDQDIHAFTVFVRDFLSKYGRWKSPKY
                     LYGESYGTMRAAGMTLALQKQDIDLNGVILLSDILNWDLLPDDPQVNPGVDLPYIVTL
                     PTYAATAWYHKRAGTGRDLASWLAEAEAFATGDYALALMKGNDLSDAERQTIAARLHA
                     YTGLPVDYLMKTDLRIQYGAMQKELLADEGLTTGTLDTRFTGATLDPLSKVASNDPQS
                     AAIGAAYTAALNDYVRGTLGYGAGMHYEGGLDVYSSWDYKHRPPGAGRALIALPNVLP
                     DLAVAMKQNPKMKLLVTGGYFDVSTPYFAGRYELRHLPVPKALQANIAYKYYPAGHMI
                     YVKPELLKDMRTDVADFIRRTDGID"
     gene            2319..3218
                     /locus_tag="VN034_05900"
     CDS             2319..3218
                     /locus_tag="VN034_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWV60164.1"
                     /translation="MRPDSPSPVIPFLIACAGIATYSAMDVLMKGLSIDIGAYNAVLW
                     RTGAGTLLSALLYFASRPKWPGRATLRIHAWRSLFVAGMALCFFWALARLPIAEAIAL
                     AFVAPLMALYMAAIFLGEKIGPRSIAASLLGLAGVIVIVAGKLGGDYSDEALWAVGAV
                     FLSAIFYAYNLILARRQAKMAEPMEIAFFQNFFVAAILALGAPWFLAVPDAGQAPNIV
                     GAAVLATSSLLLLSWAYARAETQILATTEYTGFLWAMLFGWFFFDEAVTLPTLTGAVL
                     IVAACLIVARKAPHGDPVDPAAA"
     gene            complement(3324..>3610)
                     /locus_tag="VN034_05905"
     CDS             complement(3324..>3610)
                     /locus_tag="VN034_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HWV60165.1"
                     /translation="EIVAPGMLASHYAPGKPVRLGAVEFADDEFGIGFGAIAGDYDLS
                     ASGDLTEAAARLFDALHAGAASAKAKIAVAAIPAEGLGAAINDRLARAAV"
BASE COUNT          599 a         1312 c         1099 g          600 t
ORIGIN      
        1 cgcgatcgag gcgctcggcc gcaaggcaaa ggcctatcag gcgtcggtca ccgacgaggc
       61 ggcctgcgcc gcgatggtcg ccgcggtcga agccgacttc ggccccatgt cgatcctcgt
      121 caacaacgcc ggcatcgcga gccgcgggct ctcggtagcc gacaccgacc ccaccgaggt
      181 cgacaagctc cttgccgtcc acgccgcggg cccgcaccgc ctgtcgcgcc tcgcgctgcc
      241 gcagctgcgc cagcataagc gcagcgacat catcgtcatt tcgagcatcg cgacctatac
      301 ccactcgccg aacggcgcgc cctacacgat ggcgaaggcg gcgggcgaag ccctcgccta
      361 tgcgctcgcc aaggaggaac tcggcaacgg cgtgcgcgtc aacatcgtcg cccccgcgct
      421 caccgtcagc gacatgggcg agaaactgtc gcgcgcgatc accggcaaca ccgacatcca
      481 ccacctcgac accaaaatgc ccttcggccg cgtcgccgtt cccgccgacg tcgcggcggc
      541 ggtggtgtgg ttcgtcagcg atgcgaaccc ctattgctcg gggcaaaaac tcaatatcga
      601 cggggcagga caggcgacat tccgatgaaa catatgcttt ttgtggcgac cttcgccctc
      661 gcgctcggcg gctgcgcgac ggcgcaatcg aaagaggcgc gcgccgccgc cgaaacaccc
      721 gcgcccgacg cgccgatgtt cgctcccacc gaggtcgaaa gccgcggatc ggtgaccgtc
      781 ggcggggtgc cgatccccta tcgcgcggtc gcgggcacgc tcgtcgtcca tccgcaaggc
      841 tgggacgaca ccgtgccgat cgagcgcaag cgcgacaagg atgccgccgc agacgtgccg
      901 aactccgaag cgtcgatgtt ctatgccgcc tatttcaagg aaggcgcgcc cgccgagagc
      961 cggccgatca ccttcctctt caacggcggt ccgggttcgc cgactttatg gctccatatg
     1021 ggcgcctatg gcccggtgcg catcgagact cccgacctga cccacggcag cgccccctat
     1081 cgcaccgtcg ccaatgatca gagcctgctc gacgtatccg acctcgtctt cgtcgacgcc
     1141 cccggcaccg gtttcagccg cgtcgcgggg aaggacaagg acaaggcgtg gttcggcgtc
     1201 gaccaggaca tccacgcctt caccgtcttc gtccgcgact tcctgtcgaa atatggccgc
     1261 tggaaatcgc ccaaatatct ttacggcgaa agctatggga cgatgcgcgc ggcggggatg
     1321 acgctcgcgc tgcagaaaca ggacatcgac ctcaatggcg tgatcctgct ttcggacata
     1381 ttgaactggg acctcctccc cgacgacccg caggtcaatc cgggggtcga ccttccctat
     1441 atcgtgacgc ttccgaccta tgccgcgacc gcctggtatc acaaacgcgc cggcacgggc
     1501 cgcgatctcg cgtcgtggct cgccgaggcc gaagccttcg cgacgggcga ttatgcgctc
     1561 gcgctgatga agggcaacga cctttccgac gccgagcggc agacgatcgc ggcgaggctc
     1621 cacgcctata ccggactgcc ggtcgactat cttatgaaga ccgaccttcg tatccaatat
     1681 ggcgcgatgc aaaaggagct gctcgccgac gaggggctga ccaccggcac gctcgacacg
     1741 cgcttcaccg gcgcgacgct cgatccgctg agcaaggtcg cgtcgaacga tccgcaaagc
     1801 gccgcgatcg gcgccgccta taccgccgcg ctcaacgact atgtccgcgg aacgctcggc
     1861 tatggcgcgg gcatgcacta tgagggcggg ctcgatgtct attccagttg ggactacaaa
     1921 caccgcccgc ccggcgccgg ccgcgcgctg atcgcgctgc ccaacgtcct ccccgacctt
     1981 gccgtcgcga tgaaacagaa cccgaagatg aaattgctcg tcaccggcgg ctatttcgac
     2041 gtctcgaccc cctatttcgc gggccgctac gaactgcggc acctgcccgt ccccaaggcg
     2101 ttgcaggcga atatcgcgta caaatattat cccgccggcc acatgatcta tgtgaaaccc
     2161 gagctgctga aagacatgcg cacggacgtc gccgacttca ttcgccggac cgacgggatc
     2221 gactaggacc aatcgacatt cagaagatgg cgcgtcgaaa atgctgaatg tcgattggtc
     2281 ctagcgcccg tccgcgcaaa ctccgctaga gcgaccccat gcgccccgat tcgccctcgc
     2341 ccgtcatccc cttcctgatc gcctgcgcgg ggatcgcgac ctattcggcg atggacgtgc
     2401 tgatgaaggg gctgtcgatc gacatcggcg cctataacgc cgtgctctgg cgcaccggcg
     2461 ccggcacgct gctgagcgcc ctcctctatt tcgcgagccg cccaaaatgg cccggccggg
     2521 cgacgctgcg gatccacgcc tggcgctcgc tcttcgtcgc ggggatggcg ctctgcttct
     2581 tctgggcgct cgcgcggctg ccgatcgccg aggcgatcgc gctcgccttc gtcgcgccgc
     2641 tgatggcgct ctacatggcg gcgatcttcc tcggtgagaa gatcggcccg cgctcgatcg
     2701 cggcgtcgct gctcggcctc gcgggggtga tcgtcatcgt cgcgggcaag ctcggcggcg
     2761 attattcgga cgaagcgctg tgggcggtcg gcgcggtgtt cctctcggcg atcttttacg
     2821 cctacaacct catcctcgcg cggcggcagg cgaagatggc cgagccgatg gagatcgcct
     2881 tcttccagaa tttcttcgtc gccgcgatcc tcgcgttggg ggcgccatgg ttcctcgccg
     2941 ttcccgacgc gggccaggcc ccgaacatcg tcggcgccgc cgtcctcgcg accagctcgt
     3001 tgctgctcct gagctgggcc tatgcccgcg ccgagacgca gatcctcgcg acgaccgaat
     3061 ataccggctt cctctgggcg atgctcttcg gctggttctt cttcgacgaa gcggtgacgc
     3121 tgccgacgct caccggcgcg gtgctgatcg tcgccgcctg cctgatcgtc gcgcgcaagg
     3181 ccccgcacgg cgatcccgtg gatccggcgg cggcgtgacc ctctcccctc ccatttatgg
     3241 gaggggtcgg gggagggcat gtcgatgtgg ggagttgccg gaacaggccc tcccctaacc
     3301 cctcccgcaa gcgggagggg gacttagacc gccgcccgcg ccagccggtc gttgatcgcc
     3361 gcgcccagcc cctcagccgg gatcgccgcc accgcaatct tcgccttcgc actcgccgcc
     3421 cccgcatgca gcgcgtcgaa cagccgcgcc gccgcctcgg tcagatcgcc gctcgcgctc
     3481 agatcataat cgcccgcgat cgccccgaac ccgatcccga actcgtcgtc ggcgaattcc
     3541 accgccccca gccgcaccgg cttgcccggc gcataatggc tcgcgagcat ccccggcgcg
     3601 acgatctccg
//
LOCUS       DATMSU010000232         4198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_13636, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000232 DATMSU010000000
VERSION     DATMSU010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13636"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VN034_05910"
     CDS             complement(<1..243)
                     /locus_tag="VN034_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HWV60166.1"
                     /translation="MNAPIGRLAESLTLPQPYLLFLGDTTEAGYAKTAFGLADWAADR
                     CVGEYGIGACSVSAGLPRLSPAEARAAGAQSLVIGVA"
     gene            complement(264..2450)
                     /locus_tag="VN034_05915"
     CDS             complement(264..2450)
                     /locus_tag="VN034_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60167.1"
                     /translation="MNKGLFVAASMVAVIAAMPAEAQEAAAIEAAGEIVVTAQKRVQN
                     VQDVPISIAVVSGEELQEQGSASLVDYAGYVPGMNVSNGGTPGQTTITLRGVAPLNAS
                     QTVGIYLDDAPVGSSAIYNRAGQFTIDLMPYDLERIEVLKGPQGTLYGASSIGGLVKY
                     VTVQPDTREFKVRAGVEGFTIKDASGLGWGGQVYVNAPIATDTLAISGSFAWRKTPGW
                     VDSVNNAAIKDQNDYEQRGGRAALLWQPTPELSVKLAGIWQSLDSDGNGIYAADLTGE
                     RLGNGRSYNNYIPESYDVDLDYYSATIDYDFGAATLTSATTYSETQSRQLQDASYAFG
                     VVLPFLPGGPTEPGITPFALNLGLKKWTQEVRLASPSGDRFEWLIGGFFTDEKTSNSQ
                     LVRSFDMAGNPIVAYDPLAIVGLPATYKEYAVFGNATFKLSERFEITGGLRWARNEQT
                     FTQISSGAIVPTANDPGKSAESVWTFSVSPQFHINEDAMLYARVATGYRPGGPNVIVP
                     NVPPSVDADRMTNYEVGLKADFADRMVSVDAAFFWMDWTDIQVTRAFGGVSGGANGGK
                     ATSKGFEGSLALRPAPGLTISATGSYTDASLSEDVPDISGLDGDRLPAVPKFSGALRA
                     DYQFELGGGNTGSFGAGVRHASNRLSLVESDPLVARAKAYTSVDLNASVTFADHWTLR
                     AYARNLLDNKGEMARSTLTNGFNQPSFLAISPLQPRTIGVALDMAF"
     gene            complement(2455..3597)
                     /locus_tag="VN034_05920"
     CDS             complement(2455..3597)
                     /locus_tag="VN034_05920"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HWV60168.1"
                     /translation="MTGSFRMTRRAALAGAMGVTLSAPMVGRSGYAFAAAPAKSYSRR
                     AVDLVASSLVIDMLAPLKITMSPDYVAHQLTDAEAAEFRASGITGFHQAYGLGGPDAK
                     TQALEYLAGWQGFAGRNSHVFTLVDGAADLDRAKADKKCAVIMGIQNAEHFEKIEDVA
                     LFRRLGLRCAQLTYNSQNMIGSGSTERVDGGVSDYGAAIIAEMEKQKMLVDVSHCGDK
                     TTLDAIEIAKGPIAITHSNARALVDHPRVKTDEAIKALAAKGGVMGITGVRMFVRTSD
                     PTNVGHMADHIDHVAKLVGIDHVGIGSDADLHGYDDMQPDEYAALKGSYKGSYAFRDK
                     IDIDGFDHPLKTFDLVEELIRRNYSNDNIRAVLGGNFRRLLAQVWG"
     gene            complement(3679..>4198)
                     /locus_tag="VN034_05925"
     CDS             complement(3679..>4198)
                     /locus_tag="VN034_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWV60169.1"
                     /translation="DILRVAAIRAARPDAWIGVDANQGYDRQTLAGLLPALVEHRVAQ
                     LEQPLKRGREADLDGLKRPLPFAADESALSLADTASLVGRFDIVNIKLDKCGGLTEGL
                     AIARQAKKLGLDVMVGNMMGTSLSMAPSYLVGQLCDIVDLDGPTFLARDRSPGVGYRG
                     GMIHCPAEIWGS"
BASE COUNT          729 a         1349 c         1343 g          777 t
ORIGIN      
        1 cgcgacgccg atcaccagcg actgtgcccc cgcggcgcgc gcctcggccg gagacagccg
       61 cggcaggccg gcgctgaccg agcaggcgcc gatcccatac tcgccgacgc agcggtcggc
      121 cgcccagtcg gcgaggccga aagcggtctt ggcatagccc gcctcggtcg tgtcgccgag
      181 gaacagcaga tagggttgcg gcagggtcag gctttcggcc agtctgccga tgggcgcgtt
      241 catcttgtcc aactccaatt cgctcaaaaa gccatgtcga gcgcgacgcc gatcgtgcgc
      301 ggctgcaacg gcgagatcgc aaggaagctc ggctggttga agccgttggt gagcgtcgat
      361 cgcgccatct cgcccttgtt gtcgagcagg ttgcgcgcgt aggcgcgcaa cgtccagtgg
      421 tcagcgaaag tcaccgacgc attgaggtcg accgacgtat aggccttcgc gcgcgccacc
      481 aggggatcgc tttcgaccag cgacaggcga ttgctcgcgt ggcggacacc ggcgccgaag
      541 ctgcccgtgt taccgccgcc cagctcgaac tggtaatcgg cgcgcagcgc gccgctgaac
      601 ttgggcaccg ccggcaggcg gtcgccgtcg agcccgctga tgtcgggaac atcctcgctg
      661 aggctcgcgt cggtatagct gccggtggcg ctgatcgtca ggccgggcgc cggtcgcagc
      721 gcgaggctgc cctcgaaacc cttgctcgtc gccttgccgc cgttcgcgcc gcccgacacg
      781 ccgccaaagg cgcgcgtgac ctggatgtcg gtccagtcca tccagaagaa ggcggcgtcg
      841 accgacacca tgcggtcggc gaaatcggct ttcagcccga cctcgtaatt ggtcatgcga
      901 tcggcatcga cgctcggcgg cacgttggga acgatgacgt tcgggccgcc ggggcgatag
      961 cccgtcgcga cgcgggcgta gagcatcgcg tcctcgttga tgtggaattg cgggctgacc
     1021 gaaaaggtcc agacgctttc ggcggattta cccggatcgt tggcggtcgg cacgatcgca
     1081 cccgagctga tttgagtgaa ggtctgttcg ttgcgcgccc agcggaggcc gccggtgatc
     1141 tcgaaccgct cgctcagctt gaaggtcgcg ttgccgaaga ccgcatattc cttgtaggtc
     1201 gcgggcaggc cgacgatggc gagcggatca taggcgacga tcggattgcc cgccatatcg
     1261 aaggacctaa cgagctgcga gttgctcgtc ttctcatcgg tgaagaagcc cccgatcaac
     1321 cactcgaaac ggtcgccgct gggcgaggcg agccgcactt cctgcgtcca tttcttgagc
     1381 ccgaggttga gcgcgaaggg cgtgatgccc ggttcggtgg gtccaccggg aagaaacggc
     1441 agcacgacac cgaaagcgta gctcgcgtcc tgaagctgcc ggctctgcgt ctcgctgtag
     1501 gtcgtcgccg aggtcagcgt ggcggcgccg aaatcatagt cgatcgtcgc cgaataataa
     1561 tcgagatcca cgtcgtagga ttcggggata taattgttgt acgaacggcc gttgcccagc
     1621 ctctcgccgg tcaggtcggc ggcatatata ccgttgccgt ccgaatcgag cgattgccag
     1681 atgcccgcga gcttgacgct gagttcgggc gtcggctgcc acagcagcgc cgcgcgtccg
     1741 ccgcgctgtt cataatcatt ctggtccttg atcgccgcat tgttgacgct gtcgacccag
     1801 cccggcgtct tgcgccaagc gaagctgccc gaaatcgcga gcgtatcggt cgcgatcggc
     1861 gcgttgacat agacctgccc gccccagccg aggccgctcg cgtccttgat ggtgaagcct
     1921 tcgacgcccg cgcgcacctt gaattcgcgc gtgtcaggct ggacggtgac atatttgacg
     1981 aggccgccga tcgaactggc gccgtagagc gtgccctgcg gccccttcag cacctcgatg
     2041 cgttcgagat catagggcat caggtcgatc gtgaattgcc cggcgcggtt atagatcgcg
     2101 ctcgatccga ccggcgcatc gtcgagatag atgccgaccg tctggctggc gttgagtggt
     2161 gcgacgccgc gcagcgtgat cgtcgtctgg cccggcgtgc cgccgttgct tacgttcatg
     2221 ccgggaacat agcccgcata gtcgacgagc gaggccgaac cctgttcctg caattcctca
     2281 ccgctgacga cggcgatcga gatcggcacg tcctgcacat tctggacgcg cttctgcgcg
     2341 gtcacgacga tctcgcccgc ggcttcgatc gcggccgctt cctgcgcctc tgccggcatc
     2401 gcggcgatca ccgcgaccat gcttgctgca acgaaaagtc ccttgttcat cgtcttaccc
     2461 ccagacttgg gcgagcaggc ggcggaaatt gccaccgagc acggcccgga tgttatcgtt
     2521 cgaataattg cggcgaatga gttcctcgac gaggtcgaag gtcttcaatg gatggtcgaa
     2581 accgtcgatg tcgatcttgt cgcgaaaggc gtagctgccc ttgtagctgc ctttgagcgc
     2641 ggcatattcg tcgggctgca tgtcatcata accgtgaagg tcggcgtcgg agccgatccc
     2701 gacatggtcg atgccgacga gcttcgcgac atggtcgata tggtcggcca tgtgcccgac
     2761 attggtggga tcgctcgtgc gcacgaacat gcgcacgccg gtgatgccca tcaccccgcc
     2821 cttcgccgct agcgccttga tcgcttcgtc ggtcttgacg cgcggatgat cgacgagcgc
     2881 gcgggcgttg ctgtgggtga tcgcgatcgg ccccttggcg atttcgatcg cgtccaatgt
     2941 cgtcttgtcg ccgcaatgcg agacatcgac gagcatcttc tgcttttcca tctcggcgat
     3001 gatcgccgcg ccatagtcac tgacaccgcc gtcgacgcgc tcggtgctgc ccgagccgat
     3061 catattctgg ctgttgtagg tgagctgcgc gcagcgcaga ccaaggcggc ggaacagcgc
     3121 cacatcttcg atcttttcga aatgctcggc attctggatg cccatgatga ccgcgcattt
     3181 cttgtcggcc ttggcgcggt cgagatcggc ggcgccgtcg acgagcgtga aaacatggct
     3241 gttgcgcccg gcgaagccct gccacccagc cagatattcg agcgcctggg tcttcgcatc
     3301 ggggccgccg agaccatagg cctgatggaa accggtgatg cccgatgccc ggaactcggc
     3361 cgcctcggca tcggtcagct ggtgcgcgac atagtcgggc gacatggtga tcttgagcgg
     3421 cgcaagcatg tcgatcacga gcgaggatgc gacgaggtcg accgcgcggc gcgaatagct
     3481 cttcgcaggc gccgccgcga aggcgtagcc actgcgaccc accatcggcg ccgacaaagt
     3541 aacccccatc gcgcccgcga gtgccgcgcg gcgcgtcata cggaacgacc cggtcattcc
     3601 atcctccatc ttgaatatct tatttgccga ctattgtcct aatcagaaaa ttgtcaaccc
     3661 atttaggaca tcaaattgtc acgaacccca aatttctgcc gggcaatgaa tcatcccgcc
     3721 gcgatatccg acgcccggcg aacggtcgcg cgcaaggaag gtcgggccgt cgagatcgac
     3781 gatgtcgcaa agctgcccga ccagatagga cggcgccatc gacaagctgg tgcccatcat
     3841 attgccgacc atcacatcga gcccgagctt cttcgcctgc cgcgcgatcg cgaggccctc
     3901 ggtcagcccg ccgcatttat cgagcttgat attgacgatg tcgaagcgcc caacgagcga
     3961 ggcggtgtcg gcgagcgaga gcgcgctttc gtcggcggcg aagggcagcg gccgcttcaa
     4021 tccgtcgaga tcggcctcgc gcccgcgctt cagcggctgt tcaagctgcg cgacgcgatg
     4081 ctcgacaagc gcgggcagca agccggcgag cgtctggcgg tcatagcctt gattggcatc
     4141 gacgccgatc cacgcgtcgg gccgcgcggc acggatcgcc gcgacgcgca atatatcc
//
LOCUS       DATMSU010000233         5578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_51635, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000233 DATMSU010000000
VERSION     DATMSU010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51635"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VN034_05930"
     CDS             <1..801
                     /locus_tag="VN034_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV60170.1"
                     /translation="QAGRWLPAIASGDAIVALAIDEGAKHRPDRIATTAMRAGNGFRL
                     DGKKSFVLHGHVADMSIVAAKTDGGITLFAVPKDAKGLTADPRRLVDSSLASHVTLDG
                     VEVDADAVIGEVDAGGEILDALLAATRAGSAAELVGVGQGAMDMTVTYLKERKQFGKL
                     IGEFQGLQHRAAHLYGEMEVARATVMKAQQLLDEGSEGARLMVSVAKAKAGRAANLAV
                     REGVQMHGGIGMTDEYDIGLYMKRDRALAEYMGDVHYHIDQVARMNGY"
     gene            957..1760
                     /locus_tag="VN034_05935"
     CDS             957..1760
                     /locus_tag="VN034_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60171.1"
                     /translation="MNLQNMFGLDGRIALVTGGSRGIGKMIVEGYLAAGAARVYISAR
                     KSAQIEEAVADFETRYPGKVIGLPVDLSTVEGCRALTKELEAREERLDILVNNAGAAW
                     GEPFEGFPEAGWDKVMDINVKSPFFLTQALHGLLKAGGSADRPAKVINIGSIDGQRLN
                     PWETYSYHASKAAILYLTKRLAARLVTDHILVTAIAPGAFQSDMNKAARDHGDAVAKS
                     IPVKRIGVPEDMAGAAIFLASKAGDYVVGDTITVDGGLVHGDVRTSIDA"
     gene            1839..2738
                     /locus_tag="VN034_05940"
     CDS             1839..2738
                     /locus_tag="VN034_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HWV60172.1"
                     /translation="MSNKPQTIAQFFKRFPTEEACLNHLFDVRFGQGFECPKCERASK
                     WFRIKAERAYSCQWCGHHLHPTVGTPFEKTRTSLQLWFYAIFLFTTTRNGVAAKELQR
                     QLGVTYKTAWRMADLIRKHMGDVDGDELLGGAGEVVEIDETIVGGSVSGKGNGYMGNK
                     SVVVGMLERDGKIVTRVVTSRTKAAMRSVILEQVQPGSTISTDEMGGYKDIAHSGYRH
                     IRVNHNRGQYADAVTGASVNGLESFFAQLKRSIIGTHIHVSRKHLWKYAKEAEYRFNR
                     RSQPSAMLDELLSTFGPLPQPRG"
     gene            2855..3043
                     /locus_tag="VN034_05945"
     CDS             2855..3043
                     /locus_tag="VN034_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60173.1"
                     /translation="MSRPRHSSRQTTRSWLSGLAITVTTLVGLFGFWRLSPGATFVIA
                     AIAFVGLIVALWVAGKEA"
     gene            complement(3040..3903)
                     /locus_tag="VN034_05950"
     CDS             complement(3040..3903)
                     /locus_tag="VN034_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60174.1"
                     /translation="MSDSQISLYFQLREGEKADLEIVSAAAIAWVESLRAVARAYDPN
                     SDVKVELVDADESSLIYNTLIDWFERNIEKRIERLDRGGGRLPRTRKLALALALFLIV
                     TGYPTYDFYFGDEGFTDEDRQLMQEILEHAKKDSAVETSKRKFFRTLEREPAITAVGI
                     KEHPKAEPLVTVQSDRFAEASGLWVTDDSDVPEQVTHPVLEVVLVKPALVHTPRAWTF
                     KPEGLPEFEAVMRDPLVLQAIQENGLPERLREGIPMTIRLEVREVLVDGQWKLVRGGR
                     SVIRVISPKLD"
     gene            4114..4629
                     /locus_tag="VN034_05955"
     CDS             4114..4629
                     /locus_tag="VN034_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60175.1"
                     /translation="MRLAFLIPPMMLLAACGSEERKSEGTEVSINAEGDGGGVKIRTG
                     KDGGKISIGGEGAAINIDVPDFVDLDIEGDFDIDGVKLYPGSQVTKVDVNASDKAGAD
                     KATVKLGFTSPAAPTKAADWMAGEFAKKGVTVTRSGDTLAGKDKDGADFTIKFAPDGA
                     NAKGEVLIVKS"
     gene            complement(4703..>5578)
                     /locus_tag="VN034_05960"
     CDS             complement(4703..>5578)
                     /locus_tag="VN034_05960"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWV60176.1"
                     /translation="GGSAFAAIRAQPAGTVQGRIRITEQGEVIAAKYGTIDSAATNLE
                     AMVSASLLASLEPEAKSDADSARFAEAMDALSDTAFGAYRGLVYDTPAFRDFFRAMTP
                     IAEIAGLKIGSRPSSRTKSSAIEDLRAIPWVFSWAQSRAMLPGWYGTGEAFAGFGDKA
                     LLTDMAQSWPFFAALLGNMEMVLAKSDMGIAARYAELAAGVDGHAELFTRIRDGWHRA
                     HDGLLDITGQTRLLEKNPALEASIRLRLPYIEPLNLLQIELMKRHRAGETDPRIAEGI
                     QLTINAIATALRNSG"
BASE COUNT         1091 a         1784 c         1703 g         1000 t
ORIGIN      
        1 caggcgggcc gctggctccc cgcgattgca tcgggcgacg cgatcgtcgc gcttgcgatc
       61 gacgaaggcg caaagcatcg ccccgaccgc atcgcgacca ccgcgatgcg cgcgggcaac
      121 ggcttccgtt tggacggcaa gaagagcttc gtccttcacg gccatgtcgc cgacatgagc
      181 atcgtcgcgg cgaagaccga cggcggcatc accctgttcg cggtgccgaa ggatgcgaag
      241 ggtctgaccg ccgacccgcg ccgcctcgtc gacagtagct tggccagcca tgtcacgctg
      301 gacggcgtcg aagtcgatgc cgacgccgtg atcggcgagg tcgacgcagg cggtgagatc
      361 ctcgacgcac tgcttgccgc cacccgcgcc ggatcggcgg ccgaactcgt cggcgtcggg
      421 cagggcgcaa tggacatgac cgtcacctac ctcaaggaac gcaagcagtt cggcaaactc
      481 atcggcgagt tccagggcct ccagcaccgc gccgcacatc tctacggcga gatggaagtc
      541 gcgcgcgcca ccgtaatgaa ggcgcagcaa ttgctcgacg agggcagcga gggtgcaagg
      601 ctgatggtct cggtcgcgaa agccaaggcg ggccgcgccg ccaatctcgc ggtgcgcgaa
      661 ggcgtccaga tgcacggcgg catcggcatg accgacgaat atgacatcgg actctacatg
      721 aaacgcgacc gcgctctcgc cgaatatatg ggcgacgtgc attaccacat cgaccaggtc
      781 gcccgaatga acggctacta aggcgcaatt acgccctccc cttcagggaa gggcagcgag
      841 acttgccagc ttgctggctg agtcgcagcg ggtggggccc agcggcctcg tgctacgccg
      901 cgagccccca ccccaacccc tcccctgaag gggaggggct tagaaaggat agcgttatga
      961 acctccaaaa catgttcggc ctcgacggcc gcatcgccct cgtcaccggc ggctcgcgcg
     1021 ggatcggcaa gatgatcgtc gagggctatc tcgccgcggg cgccgcgcgc gtctacatct
     1081 cggcacgcaa gagcgcgcag atcgaggaag ccgtcgccga tttcgaaacg cgctatccgg
     1141 gcaaggtcat cgggcttccc gtcgacctct cgaccgtcga gggctgccgc gcgctcacca
     1201 aggaactcga agcgcgcgaa gaacgcctcg atattctcgt caacaacgct ggcgccgcgt
     1261 ggggcgaacc ctttgaaggc ttccccgagg cgggctggga caaggtcatg gacatcaacg
     1321 tcaaatcgcc cttcttcctg acgcaggcgc tccacgggct gctcaaggcc ggcggcagcg
     1381 ccgaccgccc ggcgaaagtc atcaacatcg gctcgatcga cggccagcgt ttgaatccgt
     1441 gggaaaccta cagctatcac gcttcgaagg ccgcgatcct ctacctcacc aagcgcctcg
     1501 ccgcgcggct cgtcaccgac catatcctcg tcaccgcgat cgcccccggc gcctttcagt
     1561 cggacatgaa caaggccgcg cgcgaccatg gcgacgcggt ggcgaagagc ataccggtca
     1621 agcgcatcgg cgttcccgag gatatggcgg gcgccgcgat cttcctcgcg tcgaaggcgg
     1681 gcgactatgt cgtcggcgac acgatcaccg tcgacggcgg gctggtgcac ggcgatgtga
     1741 ggaccagcat cgacgcctaa ttccatccct tcgtcacccc ggacttaatc ggcgattatc
     1801 cccttgccat acgtaaagaa accgcgtaaa aatgtggcat gagcaataaa ccgcaaacca
     1861 tcgcgcagtt tttcaagcgt ttcccgaccg aagaagcctg cctcaatcac ctgtttgatg
     1921 ttcgtttcgg acagggcttc gaatgcccca agtgcgagcg cgcttccaag tggttccgca
     1981 tcaaggcgga gcgggcttat tcgtgccagt ggtgcggcca tcacctgcac ccgaccgttg
     2041 gaacgccgtt cgaaaagacg cgcacgtcgc tgcaactgtg gttctacgcg atcttcctct
     2101 tcacgacgac gcgcaacggc gtggcggcga aggaattgca gcgccagctt ggcgtcactt
     2161 acaagaccgc gtggcgcatg gccgatctta tccgcaagca tatgggcgac gtggacggcg
     2221 acgagttgct tggcggcgct ggcgaggtcg tggagatcga cgaaacgatt gtcggcggtt
     2281 cggtgtccgg taagggcaac ggctacatgg gcaacaagtc ggtcgtcgtc gggatgctgg
     2341 agcgtgacgg caagatcgtc actcgcgttg tcacgtcgcg cacgaaggcg gccatgcgct
     2401 cggttatcct agagcaggtc cagcccggtt cgacgatcag caccgacgaa atgggcggct
     2461 ataaggacat tgcccattcg gggtatcgcc atatccgagt gaaccacaat cgcggccagt
     2521 atgcagacgc cgttaccggc gcgtcggtca acgggctgga gagcttcttc gcgcagctta
     2581 agcggtcgat catcggcacc cacattcatg tgagccgcaa gcacctgtgg aaatatgcga
     2641 aggaagcaga ataccgcttc aatcgtcgga gccaaccttc ggcgatgctg gacgaacttc
     2701 tttcgacgtt tgggccattg cctcaaccgc gcggttgaac gcggcttcat cgccttgcaa
     2761 ttcgggatgc tcggcgatga actggtcgag tttcccggtg tcgagggctt gcttaagggt
     2821 caccattatg ggattctatc acaaaggaaa ccgaatgtcc cgcccgcgcc attctagccg
     2881 ccagacgacc cggtcatggc tttctggcct agcaattacc gtcacgacgc tggttggctt
     2941 gttcggcttc tggcgcctgt cgccgggcgc gacttttgtg atcgcggcca ttgcttttgt
     3001 cggcttgatc gtcgcgttgt gggtagctgg gaaggaagct taatccagct tgggactgat
     3061 cactctgatc accgagcgcc cacctctgac tagcttccac tggccatcaa ccaacacttc
     3121 acggacttcc aacctgatag tcattgggat gccctcccgc agcctttcgg gcaggccatt
     3181 ttcctgtatc gcctgcaaga caagaggatc gcgcatcaca gcctcgaact cgggcaatcc
     3241 ttcgggtttg aacgtccagg cgcggggggt gtgaacaaga gcaggcttca ccagtacgac
     3301 ctccaagaca ggatgggtga cctgctcggg tacatcgcta tcatcagtaa cccataggcc
     3361 gctcgcctca gcgaagcggt cactttgcac ggtgacgagc ggctcagcct tcggatgctc
     3421 tttgatgcct acagccgtga ttgcgggctc acgttcaagg gttcggaaga acttccgctt
     3481 tgaggtttcg acggcggagt ccttcttcgc gtgctccaat atctcttgca tcaactgacg
     3541 atcttcgtcc gtgaagccct catcgccgaa atagaaatcg tatgtcgggt agccggtcac
     3601 gatcagaaat agggccagtg ccaaagccag ttttcgtgtg cgcggaaggc ggcccccgcc
     3661 acgatccagc cgttcgatcc gcttttcaat attgcgctcg aaccagtcga taagcgtgtt
     3721 gtatatcagg ctgctttcgt cggcatcgac aagctcaacc ttcacgtcac tgttgggatc
     3781 gtaggcgcga gcgaccgcgc gtaacgattc aacccaagcg atagcggccg ccgatactat
     3841 ttcgaggtcg gccttttcac cttcccgcaa ctgaaaataa agactgattt gactatcgct
     3901 catccctaat cccctcgcgg ggaaactgcc tcggagctac tttagagtca atctccgtat
     3961 gcaaagggga taatcgccga cttaatccgg ggtcccgcta tgtgacctcg aaaaagcggg
     4021 atgccggatc gaggccggca tgacgaacga ggatagattg actcaccaat ctccaaggtg
     4081 tattatacaa ctaatgcact aaggagaaga tccatgcgct tggcgttcct gattcccccg
     4141 atgatgctcc tcgccgcttg cgggtcggaa gagaggaaat cggagggcac cgaagtctcg
     4201 atcaacgccg aaggcgacgg cggcggcgtc aagatccgca ccggcaagga cggcggcaag
     4261 atcagcatcg gcggcgaggg agcggcgatc aacatcgacg tccccgactt cgtcgacctc
     4321 gacatcgagg gcgatttcga catcgacggg gtcaagctct acccgggcag tcaggtgacc
     4381 aaggtcgacg tcaatgccag cgacaaggcc ggcgccgaca aggcgacggt caagctcggt
     4441 ttcacctcgc ccgccgcgcc gacgaaggcc gccgactgga tggcgggcga attcgccaag
     4501 aaaggcgtca cggtcacgcg cagcggcgac acgctcgcgg gcaaggacaa ggacggcgcc
     4561 gacttcacga tcaaattcgc ccccgacggc gcgaacgcca agggcgaggt gctgatcgtc
     4621 aagagttgac ggccgacccg acaccaacct ccgttcgtgt ctcgacttcg ctcgacacaa
     4681 gcgggatagg gtagggccaa gcctacccgc tattccgcag cgccgtcgcg atcgcgttga
     4741 tcgtcagctg gatgccctcg gcgatgcgcg ggtcggtctc cccggcgcgg tggcgcttca
     4801 tcagctcgat ctggagcagg ttcaagggct cgatataggg cagccgcagc cggatcgacg
     4861 cctccagcgc cggattcttt tcgagcagcc gcgtctgccc ggtgatgtcg agtaagccgt
     4921 cgtgcgcgcg gtgccacccg tcgcggatac gcgtaaacag ctcggcatgc ccgtcgaccc
     4981 ctgcggccag ctcggcatag cgcgccgcga tccccatgtc ggatttggcg agcaccatct
     5041 ccatattgcc gagcagcgcg gcgaagaagg gccagctctg cgccatatcg gtcagcagcg
     5101 ccttgtcgcc aaagcccgcg aacgcctcgc ccgtcccgta ccagccgggc agcatcgcgc
     5161 gcgactgcgc ccaactgaac acccacggga tcgcgcgcag atcctcgatc gcgctcgact
     5221 tggtgcggct cgacggccgc gacccgatct tgagccccgc gatctcggcg atcggggtca
     5281 tcgcgcggaa gaaatcccgg aacgccggcg tgtcgtagac gagcccgcga taggccccga
     5341 acgcggtgtc cgacagcgcg tccatcgctt cggcgaaacg cgcgctgtcg gcgtcgctct
     5401 tcgcctcggg ctcgaggctc gcgagcaggc tcgccgacac catcgcctcg aggttcgtcg
     5461 cggcgctgtc gatcgtgcca tatttcgccg cgatcacttc gccctgttcg gtgatgcgga
     5521 tgcggccctg caccgtgccc gcgggttgcg cgcggatcgc ggcaaaggcg ctgccgcc
//
LOCUS       DATMSU010000234         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_146133,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000234 DATMSU010000000
VERSION     DATMSU010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146133"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VN034_05965"
     CDS             <1..196
                     /locus_tag="VN034_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60177.1"
                     /translation="SVAELRWMVMREWARTTDDILWRRSKLGLLFSAAETERLAGHVE
                     RIAAEARQIDAEFFEDERSA"
     gene            complement(193..852)
                     /locus_tag="VN034_05970"
     CDS             complement(193..852)
                     /locus_tag="VN034_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWV60178.1"
                     /translation="MKTEILAVLTGKARPFRGDEPSAIGKLPVADAVAVDAMGLAGDE
                     QADRSVHGGIDKAIHHYPADHYDWWRGQLGAVPLLDAAGAFGENISTIGLDENSVFLG
                     DRFRLGTALVEVTQARQPCWKLDHRFEAKGVMASVVKSRRSGWYYRVLEPGTVRAGDA
                     LELVERPYPDWPLASLFALLIGGEAKSRLADLTALRDVPALADTWKARRAKLAEQFGA
                     D"
     gene            complement(893..2056)
                     /locus_tag="VN034_05975"
     CDS             complement(893..2056)
                     /locus_tag="VN034_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60179.1"
                     /translation="MKINFPLLALATGAFGIGITEFAPMGLLPDMAEGLGVSIPAAGL
                     LVSAYALGVMLGAPLMTLATARMNRRTLLIGLMAIFTLGNFLSAIAGDYATLMVARVI
                     TSLNHGAFFGVGSVVAASLVPPERRASAVAAMFMGLTLANVIGVPLAPWVGETFGWRT
                     AFGAIAVWGLITMAALRFALPDIGRDEGGNMLAELGVLKRREVLIALALTAIGSSAMF
                     TVFTYIAPILREAAGAGTLFVTAMLVVYGLGLTAGNWLGGVFADKSIDRTLIVSLAGL
                     TATLVVFTGAMFQPLAAAVTIFVWGVATFAIVPPLQMRVMEAASDAPNLASAVNIGAF
                     NLGNAVGAAVGGGVIGLGLGYPAVSFAGAAMALAGLAIVVATRAQTSHALAKA"
     gene            complement(2070..3104)
                     /locus_tag="VN034_05980"
     CDS             complement(2070..3104)
                     /locus_tag="VN034_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWV60180.1"
                     /translation="MEYRQLGSSGLRVPALSFGTGTFGGQGPLFSAWGTSDAAEARRL
                     IDISLDAGVTLFDTADVYSNGASEEILGAAIKGRRDAVLISTKTGLPMGDGPQDWGVS
                     RSRLIGAVEDALRRLGTDHIDLLQLHAFDASTPVDELMDTLGALIAAGKLRYAGVSNY
                     PGWQLMKAQATADRLGTPRFVAHQVYYSLIGRAYEADLMPLAADQGIGALVWSPLGWG
                     RLTGKIGRGRPIPPGSRLHETEQFAPPVAEELLYGVIDAIEAVAAETGKTVPQVAINW
                     LLRRPTVSSVIIGARNEEQLRQNLGAVGWALTAEQVAELDAASDVLPPYPHTPYRQQE
                     GFARLNPPLV"
     gene            3191..>3356
                     /locus_tag="VN034_05985"
     CDS             3191..>3356
                     /locus_tag="VN034_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV60181.1"
                     /translation="MNAKIDGGGDRARSMEVFAATVAEGSFSAAGRCLGLTPSAVSRT
                     IDRIEVRLGVR"
BASE COUNT          579 a         1135 c         1158 g          484 t
ORIGIN      
        1 cagcgtcgcc gagctgcgct ggatggtgat gcgcgaatgg gcgcgcacga ccgacgacat
       61 tctctggcgg cggagcaagc tggggctgct tttcagcgcg gccgagaccg agcgcctcgc
      121 cggccatgtg gagcgcatcg cggccgaagc gcggcagatc gacgccgaat ttttcgagga
      181 cgagcgaagc gcttagtcgg caccgaactg ctcggcgagc ttggcgcggc gcgccttcca
      241 ggtgtcggcg agcgcgggca cgtcgcgtag cgcggtgagg tcggcgagcc gcgacttcgc
      301 ctcacccccg atcagcagag cgaagagcga cgcgagcggc cagtcgggat acggccgttc
      361 gacaagctcg agcgcatcgc ccgcgcgtac cgtccccggt tcgagtacgc gataatacca
      421 gcccgaccgc cgcgacttga cgacactggc catgacgccc ttcgcctcga agcgatggtc
      481 gagcttccag cacggctggc gcgcctgcgt cacctcgacg agcgcggtgc cgaggcggaa
      541 gcggtcgccg aggaagacgc tgttttcgtc gagaccgatg gtcgagatat tctcgccgaa
      601 cgcgcccgcc gcatcgagca gcggcaccgc gccgagttgg ccgcgccacc agtcataatg
      661 gtcggcggga taatggtgga tcgccttgtc gatcccgcca tggacgctgc gatcggcctg
      721 ttcgtcgccg gcgaggccca ttgcatcgac cgcgaccgcg tccgcgaccg gcagcttgcc
      781 gatcgcgctc ggctcgtcgc cgcgaaaggg gcgcgccttg cccgtcagga cggcgagaat
      841 ctcggtcttc atgcccgcga ttgcataggc cgagcggcga aaatggcaat catcaggctt
      901 tggcgagtgc gtgcgatgtt tgcgcccgcg tcgcgaccac gatcgccagc cccgcaagcg
      961 ccatcgccgc tcccgcgaac gacaccgcgg gataaccgag cccgaggccg atcaccccgc
     1021 ccccgactgc ggcgccgacg gcgttgccga ggttgaaggc gccgatattg accgccgagg
     1081 cgagattggg cgcatccgac gccgcctcca tcacgcgcat ctggagcggc gggacgatcg
     1141 caaaggtcgc gacgccccag acgaagatgg tgacggcggc ggcgagcggc tggaacatcg
     1201 cgcccgtgaa aacgacaagc gtcgcggtga gcccggcgag cgagacgatc aacgtgcggt
     1261 cgatcgactt gtcggcgaag acgccgccga gccagtttcc ggcggtgagg ccgagcccgt
     1321 agacgacgag catcgcggtc acgaacagcg ttcccgcccc cgccgcctcg cgcaggatcg
     1381 gcgcgatata ggtgaagacc gtgaacatcg ccgacgatcc gatcgcggtc agcgcgagcg
     1441 cgatcaacac ctcgcgccgc ttgagcacgc cgagttcggc gagcatgttg ccaccttcat
     1501 cacggccgat gtcggggagc gcaaagcgga gcgccgccat cgtgatcagc ccccagaccg
     1561 cgatcgcgcc gaacgccgtg cgccagccga acgtctcgcc gacccacggc gcgagcggca
     1621 cgccgatgac attggcgagc gtcagcccca tgaacatcgc cgcgaccgcg ctcgcgcgcc
     1681 tttcgggcgg cacgaggctc gccgcgacga ccgatccgac gccgaagaag gcgccgtgat
     1741 tgagcgaggt gatcacgcgc gcgaccatca gcgtcgcata atcgcccgcg atggcggaga
     1801 ggaaattgcc gagcgtaaag atcgccatca gcccaatcag cagggtgcgc cggttcatcc
     1861 gcgcggtggc gagcgtcatc agcggcgcgc cgagcatgac gccgagcgca taggccgaga
     1921 cgagcagccc cgcggcgggg atcgacacgc cgagcccctc cgccatgtcg gggagcaggc
     1981 ccatcggcgc gaattcggtg atgccgatgc cgaacgcgcc cgtcgcgagc gcgagcagcg
     2041 gaaagttgat cttcatggag gtagtcctgt cagacgagcg gcgggttcag gcgggcgaag
     2101 ccttcctgct gccgatacgg cgtgtgcgga tagggcggca gcacatcgct cgccgcgtcg
     2161 agctccgcca cttgctcggc ggtcagcgcc cagccgaccg cgccgagatt ctggcggagc
     2221 tgctcctcat tgcgcgcgcc gatgatcacc gacgacacgg tcgggcgccg gagcagccag
     2281 ttgatcgcga cctgcggcac cgtcttgccc gtttcggctg cgacggcttc gatcgcgtcg
     2341 atcacgccgt agagcagctc ctcagcgacg ggcggcgcga actgttcggt ttcgtggagg
     2401 cggctgcccg gcgggatcgg gcgcccgcgc ccgatcttgc cggtgaggcg cccccagccg
     2461 agcgggctcc acaccagcgc accgatccct tgatcggcgg cgagcggcat caaatcggct
     2521 tcataggcgc ggccgatcag cgaataataa acctgatgcg cgacgaagcg cggggtgccg
     2581 aggcggtcgg cggtcgcctg cgccttcatc aactgccagc cgggatagtt ggagacgcca
     2641 gcgtagcgca gcttgccggc ggcgatcagc gcgccaagcg tatccatcag ctcgtcgacc
     2701 ggggtcgaag cgtcgaaggc gtggagctgg agcaagtcga tatgatcggt gccgagccgg
     2761 cgcagcgcat cctcgaccgc gccgatcagc cgactgcgcg agacgcccca atcctgcggc
     2821 ccgtcgccca tcggcagtcc ggttttggtc gagatgagca ccgcgtcgcg gcgtcccttg
     2881 atcgccgcgc ccaatatctc ctccgacgcg ccgttcgaat agacgtcggc ggtgtcgaac
     2941 agggtcaccc cggcgtcgag actgatgtcg atcaatcgcc gcgcctcggc cgcgtcgctc
     3001 gtgccccacg cgctgaacag cggcccctgc ccgccgaagg tgccggttcc gaagctcaag
     3061 gcggggacgc gaagccccga cgatcccaat tgacgatatt ccatgatcga ccctttcgat
     3121 tgcgttgaaa gcatagatgg actcgaggca cttgccggag tagcgccggg acaggcgaag
     3181 gatttttgaa atgaacgcaa agatagacgg cggcggcgac cgtgcgcgat cgatggaggt
     3241 atttgcggcg accgtcgcgg agggcagttt ttcggccgcc ggccgctgcc tcgggcttac
     3301 tccgtcggcg gtgagccgca cgatcgaccg gatcgaggtg cggctcggcg tgcggc
//
LOCUS       DATMSU010000235         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_165135,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000235 DATMSU010000000
VERSION     DATMSU010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165135"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VN034_05990"
     CDS             complement(<1..247)
                     /locus_tag="VN034_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV60182.1"
                     /translation="MHFAFTDEQAMIAETAKAFFAEQATSERTRAAMAASGIDEALWQ
                     AFTAELGLAGVAIPENQGGVGLGMVELAILAEEAGRHV"
     gene            complement(249..1151)
                     /locus_tag="VN034_05995"
     CDS             complement(249..1151)
                     /locus_tag="VN034_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60183.1"
                     /translation="MGICDGRTVIITGAARGLGRSYALAFAAEGANVVVNDIGTSLAG
                     EGRDTSAADGVVAEIKAAGGNAIANYEDITDWDAAKRIVDSAVAAFGDLHVVVNNAGI
                     VRDRMFVSATPEEWDATMHVHLRGHFCVSRHAVDYWRASQKAGKPVDARIINTTSGAG
                     LQGSIAQAAYSTAKGGIAALTLVQAAELGRYGITANALAPAARTRMTEGAFADKMAAV
                     ESGFDAQDPDNIAPTVVWLGSDQSKHVTGCVFELEGGRITIEDGWKLGPTVDQQAKWA
                     AADVGAAVDELLAERSEPRKVWGS"
     gene            complement(1157..2308)
                     /locus_tag="VN034_06000"
     CDS             complement(1157..2308)
                     /locus_tag="VN034_06000"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWV60184.1"
                     /translation="MPEAYIIDAVRTPIGRKKGILAAVHPADLAAHPIKALVERTGID
                     ANLVDDVVWGCCDTIGPQAGDIGRTAWLVAGLPEHVPGTTIDRQCGSSQQAVHFAAQG
                     VMSGTQDLVVAGGSQAMNAIPISAAMYAGQAYGFDSPFIGAKGWDARYGTEEINQIKS
                     AEMIANKWQITRAAMEEFATESHRRAQAAIDNGWFKTEIAPFEGLEQDETVRPQTTPE
                     GLATLKTVNEGGVITAGIASQNCDGAAALLIASEAAVKAHGLKPRARIHHLSVRADNP
                     VWMLTGPIPATRYALEKAGMTVGDIDLFECNEAFASIPMAWMQELGVPHEKVNVQGGA
                     IALGHPIGATGARLMTTLLGALERTGGRYGLQTMCEGGGQANVTIIERL"
     gene            complement(2319..>3179)
                     /locus_tag="VN034_06005"
     CDS             complement(2319..>3179)
                     /locus_tag="VN034_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV60185.1"
                     /translation="VLIAAELGRAGVAEPLADTALAAAPLLAKAGGQDALLAAIAAGE
                     AKIALQHPVNPWVADLDGATHILSVSDEALLLAPCSPAKAGAQETKVDGPRLSPGNTE
                     YTLLDSVDPLRRLYVPVAPAGTEAGSADALLDHAALIAAAQLVGVADAMLEQATEYAK
                     NRSQFGQPIGSFQAVKHHLATAVVKIEFAKPVIQRAAVALQENAASASVHVSHAKLAA
                     ADAAWAMSEIAIQTHGAMGYTYEVDLHFWMKRAWALAGAWGDRAFHLRRIDAAVIGDT
                     LPIGPGNTFA"
BASE COUNT          454 a         1060 c         1088 g          577 t
ORIGIN      
        1 ctacgtgccg tccggcctcc tcggcgagga tcgcgagttc gaccatgccg agcccgacgc
       61 cgccctgatt ctcggggatc gcgaccccgg cgaggccgag ttcggcggtg aaggcttgcc
      121 acagcgcttc gtcgatgccg ctcgctgcca tcgcggcgcg cgtgcgctcg ctcgtcgcct
      181 gttcggcgaa gaacgccttc gccgtttcgg cgatcatcgc ctgttcgtcg gtgaaggcga
      241 agtgcatcct agctgcccca gaccttgcgc ggttcgctac gttccgcgag caactcgtcg
      301 accgccgcgc cgacgtcggc cgccgcccat tttgcttgct ggtcgacggt ggggccgagc
      361 ttccagccat cctcgatcgt gatccgtccg ccttcgagct cgaaaacgca gccggtcaca
      421 tgcttcgact ggtccgaacc gagccagacg acagtgggcg cgatattgtc ggggtcctgc
      481 gcgtcgaagc cgctttcgac cgccgccatc ttgtcggcaa atgcgccttc ggtcattcgc
      541 gtgcgcgccg cgggcgccag cgcattggcg gtgatgccgt agcggccaag ctcggccgct
      601 tgcaccaacg tcagcgccgc gatcccgccc ttcgcggtcg aataggctgc ctgcgcgatc
      661 gatccctgga gaccggcgcc gctcgtcgtg ttgatgatgc gggcgtcgac gggcttgccc
      721 gccttttggc tagcgcgcca ataatcgacc gcgtggcggc tgacgcagaa atggccgcgc
      781 agatggacgt gcatcgttgc gtcccattct tcgggggtcg cggagacgaa catgcgatca
      841 cgcacgatgc cggcattgtt gacgacgaca tgcaggtcgc cgaacgccgc gaccgcagaa
      901 tctacgatgc gtttggcggc atcccagtcg gtgatgtcct cataattggc gatcgcgttg
      961 ccgcccgcgg ccttgatctc ggcgacgacg ccgtcggccg ccgaggtgtc gcggccttcg
     1021 cccgcgaggc tggtgccgat gtcgttgacg acgacattgg cgccctcggc cgcgaaggcg
     1081 agcgcatagg aacgcccgag cccgcgcgcc gcgccggtga tgatgacggt tcgtccgtcg
     1141 caaataccca tgggtcttac agcctttcga tgatggtgac gttggcctgg ccgccgcctt
     1201 cgcacatggt ctggaggccg tagcggccgc cggtgcgttc gagcgcgccg agcaaggtgg
     1261 tcatgaggcg cgcgccggtg gcgccgatcg gatggccgag cgcgatcgcg ccgccctgca
     1321 cattgacctt ttcgtgcggg acgccgagtt cctgcatcca cgccatcggg atcgatgcga
     1381 aggcctcgtt gcattcgaac aggtcgatgt cgcccacggt catgcccgcc ttttcgagcg
     1441 catagcgggt cgcggggatc gggccggtga gcatccagac cggattgtcg gcgcggaccg
     1501 acaggtgatg gatgcgcgcg cggggtttca ggccatgcgc cttcaccgcc gcttcgctcg
     1561 cgatcagcag cgccgcggcg ccgtcgcaat tctggctggc gatacccgcg gtgatcacac
     1621 cgccctcgtt gacggtcttc agcgtcgcaa gcccttcggg ggtggtctgc gggcggacgg
     1681 tttcgtcctg ctccagccct tcgaaaggcg cgatctcggt cttgaaccag ccattgtcga
     1741 tcgcggcctg cgcgcggcgg tgcgattctg tcgcgaattc ttccatcgcc gcgcgggtga
     1801 tctgccactt gttggcgatc atctcggccg acttgatctg gttgatttcc tcggtgccgt
     1861 agcgggcgtc ccagcccttc gcgccgatga aggggctgtc gaagccatag gcctgccccg
     1921 cgtacatcgc cgccgaaatc ggaatcgcgt tcatcgcctg gctgccgccc gcgacgacaa
     1981 ggtcctgtgt gccgctcatc acgccctgcg ccgcgaaatg caccgcctgc tgcgacgaac
     2041 cgcactggcg gtcgatcgtc gtgccgggga catgctcggg aagccccgca accagccatg
     2101 cggtgcggcc gatgtcgccc gcctgcgggc cgatcgtgtc gcagcagccc cagacgacgt
     2161 cgtcgaccag attggcgtcg atgccggtgc gttcgaccaa tgccttgatc ggatgcgcgg
     2221 cgaggtcggc ggggtggacg gcggcgagga tgcccttctt gcgcccgatc ggggtgcgga
     2281 cggcgtcgat gatataggct tcaggcattc actgttcctc aggcgaaggt attgccgggg
     2341 ccgatcggga gcgtgtcccc gatgacggcg gcgtcgatgc ggcgaagatg gaaggcgcgg
     2401 tcgccccatg cgccggcgag tgcccacgcg cgcttcatcc agaaatgcag gtcgacctcg
     2461 taggtgtagc ccatcgcgcc gtgcgtctgg atcgcgatct cgctcatcgc ccacgcggcg
     2521 tcggcggcgg cgagcttggc gtggctgaca tgcaccgacg cgctggcggc gttctcctgc
     2581 aacgccacgg cggcgcgctg gatcaccggt ttggcgaact cgatcttcac gacggcggtc
     2641 gcgaggtgat gcttgaccgc ctgaaagctg ccgatcggct ggccgaactg gctgcggttc
     2701 ttcgcatatt cggttgcctg ttcgagcatc gcgtcggcga cgccgacaag ctgcgcggcg
     2761 gcgatcagcg cggcatggtc gaggagggcg tccgccgagc ccgcttcggt tccggccggc
     2821 gcgaccggta catagaggcg acggagagga tcgacgctgt cgagcagcgt gtattccgtg
     2881 ttccccggcg aaagccgggg tccatcgacc ttggtttcct gggccccggc tttcgccggg
     2941 gaacacggcg cgaggagcag agcttcatcg ctcaccgaaa gtatatgcgt agcgccgtcc
     3001 agatcagcga cccacggatt gaccggatgc tggagcgcga tcttcgcttc acccgcggca
     3061 atcgcagcga gcagcgcgtc ctgcccgccc gccttggcga gcagcggcgc ggcggcgagc
     3121 gcggtgtcgg cgagcggttc ggcgacacct gcacggccga gttcggcggc gatcagcac
//
LOCUS       DATMSU010000236         1926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_98930, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000236 DATMSU010000000
VERSION     DATMSU010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98930"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VN034_06010"
     CDS             <1..217
                     /locus_tag="VN034_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWV60186.1"
                     /translation="LGQKAPVADGLAVYGPAPAPLAMLRGRHRFRLLLHAPRSFDLQG
                     TIRDWVNSVEWSSKARLAIDIDPYSFV"
     gene            292..1827
                     /locus_tag="VN034_06015"
     CDS             292..1827
                     /locus_tag="VN034_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60187.1"
                     /translation="MHGVRITILAALAALTTPAQANWLRADTDSFIIYSEGNEKSLRT
                     FAENLQRFDATLGTRFGLKKAPEPNRLTIYLLPRGVDVGRLVTGKSGSFIGGWYRPDP
                     EGSYAVSHRENDVIKGTPQSQQTLFHEYSHHFMKRYAAAAYPAWFIEGFAEYYSTADF
                     TTDGRAEIGKPVHARAHGLLTMPKIPAETILLQQPGEMRNSDQTDVYYGRSWLLTHML
                     YNDPARAGQLTKYIDAINRGEDGKKAAIDAFGDLAALDKDLNRYMTKPMTYRISSEPA
                     VVSGKISITALSPAEDALLPLHLERMSGQNDEERVVKVRDDLKALSLVHGGDAGVWFE
                     LASAEWAIDEDKRDMAAARAAVDKALAIDPKHVRANVLLGRILTAEVEAKDDPGPEDW
                     NAVRKPIILANRTNPDDPVPLYAYFESFADQGRWPPPIAIEGLRRAFQLAPEDIGVRM
                     TYAFALANDERYDEALALAKAVAFDPHDGGQGEQLLARLEAMRSQGAARASAAAAEEA
                     ADE"
BASE COUNT          363 a          643 c          622 g          298 t
ORIGIN      
        1 gctggggcaa aaggcgcccg tcgccgacgg ccttgccgtc tatggccccg cgccagcccc
       61 cttggcgatg ctgcgcggcc gccaccgctt tcgcctgttg ctccacgcgc cgcgcagctt
      121 cgatttgcag gggacgatcc gcgactgggt gaacagcgtc gaatggtcat cgaaggcgcg
      181 gctcgcgatc gacatcgatc cctacagctt tgtctagccg atagagggct ttacagcctc
      241 gtcgcaggct ggcagcagaa tgcgtatcgc catccggggg gagcagcggg gatgcatggg
      301 gttcgcataa cgatattggc ggcgctggcc gcgttgacga cgccggcgca ggcgaactgg
      361 ctgcgcgccg acaccgatag tttcatcatc tacagcgagg gcaacgagaa atcgctgcgc
      421 accttcgccg agaatctcca gcgattcgac gcgacgctcg gcacgcggtt cggattgaag
      481 aaggcgcccg agcccaaccg gctgaccatc tatctgctcc cgcgtggcgt cgatgtgggc
      541 cggctggtca ccggcaagag cgggtccttc atcgggggct ggtaccggcc cgaccccgag
      601 ggcagctatg cggtctcgca ccgcgagaat gacgtgatca aggggacgcc gcaatcgcag
      661 cagacgctgt ttcatgaata tagccaccat ttcatgaagc gctacgccgc cgctgcctat
      721 ccagcctggt tcatcgaagg cttcgccgaa tattattcga cggccgattt caccacggac
      781 gggcgcgccg agatcggcaa gcccgttcat gcacgcgccc atggcctgct gacgatgccc
      841 aagatcccgg ccgagacgat attgctccag cagccgggcg agatgcgcaa ttcggaccag
      901 accgacgtct attacggccg ttcgtggctg ctgacgcaca tgctatacaa cgatccggcg
      961 cgcgcgggcc agctcaccaa atatatcgac gcgatcaacc gcggcgagga tggaaagaag
     1021 gcggcgatcg acgcgttcgg cgatctcgcg gcgctggaca aggacctcaa ccgctacatg
     1081 accaagccga tgacctaccg catctcgtcc gagccggccg tggtttccgg caagatcagc
     1141 attaccgcgc tgtcgcccgc cgaagatgcg ctgctgccgc ttcacctcga acggatgagc
     1201 ggccagaacg acgaggaacg ggtggtcaag gtgcgcgacg acctgaaagc cctgagcctt
     1261 gtgcacggcg gcgacgccgg cgtgtggttc gaactggcga gtgccgaatg ggcgatcgac
     1321 gaggacaagc gcgacatggc ggcagcccgg gccgcggtcg acaaggcgct ggccatcgac
     1381 cccaaacatg tccgcgcgaa tgtcctgctc ggccgcatcc tgaccgccga ggtcgaggcg
     1441 aaggacgatc cggggccgga agactggaat gcggtgcgta aaccgatcat actcgccaac
     1501 cgcaccaacc ccgacgaccc ggtcccgctc tacgcctatt tcgaaagctt cgccgatcag
     1561 ggccgctggc cgccgccgat cgcgatcgaa ggccttcgcc gcgcctttca gctcgcgccc
     1621 gaggatatcg gcgtccgcat gacctatgcc ttcgcgctcg ccaacgacga gcggtatgac
     1681 gaggcgctgg cactggcaaa ggcggtcgcg ttcgacccgc acgacggcgg tcagggcgag
     1741 caactgctcg cccggctcga agcgatgcgc agccaaggcg cggcgcgcgc atccgccgcc
     1801 gcagcggaag aagcagcgga cgaataaggc cggtcaataa tcgggttcgg cgccgcccat
     1861 cacctgtccg gcctgcgccg cgagccacgc catcgcggcc gcccagggct ggtgcccatg
     1921 gctgat
//
LOCUS       DATMSU010000237         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_89743, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000237 DATMSU010000000
VERSION     DATMSU010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89743"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VN034_06020"
     CDS             <1..326
                     /locus_tag="VN034_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV60188.1"
                     /translation="FDWDSTVAPADAGQSVAFMAENRTRCAWRSFTVVGHTDRSGPDS
                     YNDGLALRRAHVIADMMGGAGIPADTIAVSGMGERQPRVQTVDGQRTPENRRVEVEVN
                     GMGGQ"
     gene            326..1720
                     /locus_tag="VN034_06025"
     CDS             326..1720
                     /locus_tag="VN034_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWV60189.1"
                     /translation="MKPQHKLAGLVSALAMAFFAASAAAEPTSMQNVMAVAIDSNPQI
                     HQAEMNKQAIEFELEQAKGLYLPRVTLEMSAGVRRLENATRRALGIADDELYPLEAGI
                     RAEQMLIDFGRRRGEKLRQAARVDGAALRVVERSEFIALQSARQYLDVLLQQRVVAAA
                     EDNMTFHNSLVSDLSSGVSAGSISIADLQQAQERAKAASVRVSEAKEALQNARIELLA
                     LSGLQVDEVQMPPAMAERLPVSLSEAVGLTRARHPKVLEAQADVDASNAEAKKAKGDF
                     APTIGLELSGRIGDDIDAFRGETNDLLGRVVLRWDIFDGGINRAKYQEMVRRASESRF
                     RLHEAERNAEADTRRAWNARETQTAVFRDLVDQSKATDELLLSYRDQFGVGRRSLLDV
                     LDAQNTRFNVQVRAETARFSEMFAVYQILASTNNLLEAFNLTAPESRRVYAREEVGYG
                     PPAPAELQRRRYPQ"
     gene            1757..>3613
                     /locus_tag="VN034_06030"
     CDS             1757..>3613
                     /locus_tag="VN034_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWV60190.1"
                     /translation="MLQDRSTIAHIDREAPGDPLIACILFVAQHFGQNFQRGALVALA
                     RDERGFLPFHQAEAALDLCFINNERLDARKVRHFARALPAIVRRTDGTCAVLIEAKDE
                     QYLVWEPGAALPFWADEAAVEESYAGQAVAVAADPTAIRAEERPWDAAAKRHWFWSEV
                     RRMRRSFYPVLGAALMINLLGFALPLFTMNVYDRVIPNKAVSSLWVLAIGALLAFAVE
                     FTLRLARATLLDDLGRDLDVKLSEKIFSKVLNMPLADRRGSTGMLARRVSEFESVRDF
                     FASTTIVLIVDLAFLFLFLIMIAVLAGWLALVPLAIIAAMGVAGYFLQRRMMQASLDN
                     QADMSIQHAMLVEAIGGAETVKSCAAEGQVLGQWRRMVDTSARTQATLRRTGAAAINL
                     ATLGQQFSGLALVVGGFYLFDAGKISMGAIIAIVMLAGRSMAPVGQLAFLMTRGRQAL
                     TTMASIQQMIEAGDERRMGSSALNLTIDKGDVSVEGLCFTYPGAAAPSLTDIDLRIDA
                     GERIAVVGRVASGKSTLGRLLCGLYAPDAGNIFIDGLDSRQHSPIYLRSQFRFVGQDA
                     VLFSGSIKQNMQYCAPAATDAELLATLQSIGADRFMGRDETGLDRGTGERGGQ"
BASE COUNT          608 a         1203 c         1214 g          588 t
ORIGIN      
        1 atttcgattg ggattcgacc gtggcgccag ccgacgcggg gcaatcggtc gccttcatgg
       61 ccgaaaaccg cacgcgctgc gcttggcgga gcttcaccgt cgtgggccac accgaccgct
      121 cggggccgga tagctacaac gacgggctcg cgctgcgccg cgcgcatgtg atcgccgaca
      181 tgatgggcgg cgcgggcatt ccggcggaca cgatcgcggt gtcgggaatg ggcgagcgcc
      241 agccgcgcgt ccagaccgtc gacggccagc ggacgcccga aaaccggcgt gtcgaagtcg
      301 aagtgaacgg gatgggggga cagtgatgaa accgcaacac aagctcgccg gccttgtctc
      361 ggcgctcgcc atggctttct tcgccgcctc ggcggcggcc gagccgacca gcatgcagaa
      421 tgtcatggcg gtcgcgatcg attcgaatcc gcagatccat caggccgaaa tgaacaagca
      481 ggcgatcgag ttcgagctcg aacaggcgaa gggcctgtat ctgccgcgtg tcacgctcga
      541 aatgtcggcg ggggtgcggc ggctcgaaaa tgcgacccgc cgcgcgctcg gcatcgccga
      601 tgacgagctc tatccgctcg aagccgggat ccgcgccgaa cagatgctga tcgatttcgg
      661 gcgccggcgc ggcgagaaat tgcgccaggc ggcgcgcgtc gacggcgcgg cgctgcgcgt
      721 cgtcgaacgc tccgaattca tcgcgctcca gagcgcgcgg caatatctcg acgtcctgct
      781 ccagcagcgc gtcgtcgccg ccgccgaaga caatatgacc ttccacaaca gcctcgtctc
      841 ggacctgtcg agcggggtga gcgcgggctc gatcagcatc gccgacctcc agcaggcgca
      901 ggaacgcgcc aaggcggcga gcgtgcgcgt cagcgaagcg aaggaagcgc tgcaaaatgc
      961 gcggatcgaa ctgctcgcgc tgagcgggct tcaggtcgac gaggtgcaga tgccgccggc
     1021 gatggccgaa agacttcccg tcagcctcag cgaagccgtc ggtctcaccc gcgcgcggca
     1081 tcccaaggtg ctcgaggcgc aggccgacgt cgatgcgtcg aacgccgaag cgaagaaggc
     1141 gaagggcgat ttcgcgccga cgataggcct cgagctgtcg ggccggatcg gcgacgacat
     1201 cgatgcgttc cgcggcgaaa ccaacgacct gctcggccgc gtcgtgctgc gctgggatat
     1261 tttcgacggc gggatcaacc gcgccaaata tcaggagatg gtgcgccgcg cgagcgagag
     1321 ccgtttccga ctgcacgaag ccgagcgcaa cgccgaggcc gatacgcgcc gcgcgtggaa
     1381 tgcccgcgag acgcagaccg cggtgttccg cgacctcgtc gaccagagca aggcgaccga
     1441 cgaactgctg ctgtcgtacc gcgaccagtt cggggtcggg cggcgctcgc tgctcgacgt
     1501 gctcgatgcg cagaacacgc gcttcaacgt ccaggtccgc gccgaaaccg cgcgcttctc
     1561 ggagatgttc gcggtctatc agatcctcgc ctcgaccaac aatctgctcg aggctttcaa
     1621 tctgaccgcg cccgaatcgc gccgcgtcta tgcgcgcgaa gaggtcggtt acggcccgcc
     1681 cgcgcccgcc gagctgcagc gtcgccgtta cccgcaatga cgatcggtgg attgattcca
     1741 gatagaaaaa tcggacatgt tgcaggatcg atccacgatt gcgcatatcg atagggaggc
     1801 tccgggcgat ccgctgatcg cctgcatcct cttcgtggcg cagcatttcg ggcagaattt
     1861 ccagcgcggc gcgctcgtcg cgctcgcccg cgacgaacgc ggcttcctcc cgtttcatca
     1921 ggccgaggcc gcgctcgacc tctgcttcat caacaatgaa cggctcgatg cgcgcaaggt
     1981 aaggcacttc gcgcgcgcgc tgcccgcgat cgttcgccgc accgacggga catgcgcggt
     2041 gctgatcgag gcgaaggacg agcaatatct cgtatgggag cctggcgcgg cgctgccttt
     2101 ctgggccgac gaggcggcgg tcgaggagag ctatgccgga caggcggtcg cggtcgccgc
     2161 cgatccgacc gcgatccgtg ccgaggaacg gccgtgggac gcggccgcga agcgccactg
     2221 gttctggagc gaggtccggc ggatgcgccg ttccttctac cccgtgctcg gcgcggcgct
     2281 gatgatcaat ctgctcggct tcgcgctgcc tttgttcacg atgaacgtct atgaccgcgt
     2341 catcccgaac aaggcggttt cgagcctctg ggtgcttgcg atcggcgcct tgctcgcctt
     2401 cgcggtcgaa ttcacgctgc gcctcgcgcg tgcgacgctg ctcgacgatc tcggccgcga
     2461 tctagacgtc aaattgtccg agaagatttt ctcgaaggtg ctgaacatgc cgctcgccga
     2521 ccggcgcggc agcaccggga tgctcgcgcg ccgcgtgtcc gaattcgaaa gcgtgcgcga
     2581 tttcttcgcc tcgacgacga tcgtgctgat cgtcgacctc gccttcctct tcctcttcct
     2641 gatcatgatc gcggtgcttg cgggctggct cgcgctcgtc ccgctcgcga tcatcgccgc
     2701 gatgggcgtc gcgggctatt tcctccagcg gcgcatgatg caggcctcgc tcgacaatca
     2761 ggccgacatg agcatccagc acgcgatgct cgtcgaggcg atcggcggcg ccgaaacggt
     2821 caagagctgc gccgccgagg ggcaggtgct cggccagtgg cgccggatgg tcgacaccag
     2881 cgcgcggacg caggcgacgc ttcgccgcac gggcgccgcg gcgatcaacc tcgcgacgct
     2941 cggtcagcaa ttttccggcc tcgcgctcgt cgtcggcggt ttctatctgt tcgatgccgg
     3001 caagatatcg atgggcgcga tcatcgcgat cgtcatgctc gcgggccgct cgatggcgcc
     3061 cgtcggccag ctcgcctttc tgatgacgcg cgggcggcag gcgctgacga cgatggcgag
     3121 catccagcag atgatcgagg ccggcgacga acgccgcatg ggcagcagcg cgctcaacct
     3181 cacgatcgac aagggcgacg tctcggtcga ggggctgtgc ttcacctatc cgggcgccgc
     3241 ggcgccgtcg ctcaccgaca tcgacctcag gatcgatgcg ggcgagcgga tcgcggtggt
     3301 cggccgcgtc gcgtcgggca agtcgacgct cgggcggctg ctctgcggac tctacgcgcc
     3361 cgacgcgggt aatatcttca tcgacggact cgacagccgc cagcattcgc cgatctacct
     3421 gcgctcgcaa ttccgcttcg tcgggcagga cgcggtgctg ttcagcggct cgatcaagca
     3481 gaatatgcaa tattgcgcgc cggccgccac cgatgccgaa ctgctcgcga cgctgcagtc
     3541 gatcggcgcc gaccgcttca tgggccgcga cgaaaccggg ctcgaccgcg gcaccggcga
     3601 acgcggcggg cag
//
LOCUS       DATMSU010000238        16028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_70455, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000238 DATMSU010000000
VERSION     DATMSU010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 16028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16028
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70455"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..97
                     /locus_tag="VN034_06035"
     CDS             <1..97
                     /locus_tag="VN034_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HWV60191.1"
                     /translation="LFPDRRAVGVSARGIIVGGGSFHCSSQQLPS"
     gene            153..1004
                     /gene="aguB"
                     /locus_tag="VN034_06040"
     CDS             153..1004
                     /gene="aguB"
                     /locus_tag="VN034_06040"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HWV60192.1"
                     /translation="MTRTITVAALQLPLPGPVEPNIEAVSALVEEAAAKGAQVILPSE
                     LFEGPYFCQVEEEEFFANARPTAEHPSVVAMQALAAKLKVAIPTSYFEKDGPHYYNTL
                     AMIGPDGAIMGTYRKSHIPDGPGYEEKYYFRPGNTGFKVWDVFGTRIGVGVCWDQWYP
                     ECARAMALMGAELLFYPTAIGSEPYDADLDTSRMWRRAMQGHSVSNCMPVIAANRIGP
                     EGDANFYGHSFITDEWGDMTQAFGASETGVLVETIDLDRAAKHRAGMGFFRDRRPQLY
                     GRLVEDI"
     gene            1026..1670
                     /locus_tag="VN034_06045"
     CDS             1026..1670
                     /locus_tag="VN034_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HWV60193.1"
                     /translation="MILPEDIFTEPEDVDPETLANLGPLRRLAGIWEGQRGVDINPKA
                     DGPETREYYERIELQPIDPQANGPQLFYGLRYHLHVNTREEDIAFHDQVGYWLYEAST
                     GLILQTLAIPRGQIAIAAGHAEPDAKQLMVKAERGQSEYGICSTTFLELAFRTDAYQL
                     TIDFHDDGSWSYVSDTTLIVKGRDEPFLHRDRNRLVKIAEPDLNPWAKIVRGAA"
     gene            complement(1674..2297)
                     /locus_tag="VN034_06050"
     CDS             complement(1674..2297)
                     /locus_tag="VN034_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV60194.1"
                     /translation="MNQRRFSDPVEEQDIAASSDRREGDRYRTVWRIAKVMRNGDAGL
                     WRVRNISDRGMMLAADVPIAVGEKLEIALSDTVVIRGEVVWSDEGRCGVSFDQEVDVA
                     DVLKQLAAEQRATGYRQPRLPVHTKAEAVTDEGAATHIELVDLSQNGAGFVHDGHFEV
                     GKEFDLILAGGVKRRAIVRWSRGGRGGLWLTQPLDRADLESIRRFES"
     gene            complement(2569..3321)
                     /locus_tag="VN034_06055"
     CDS             complement(2569..3321)
                     /locus_tag="VN034_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp/Glu racemase"
                     /protein_id="HWV60195.1"
                     /translation="MTRPIRIGQIVPSSNVTMETEIPALLRAREGIAPERFTFHSSRM
                     RMKKVTKEELAAMDADSDRCALELSDAAVDVLGYACLVAIMSMGEGYHRVSEARLHQR
                     TVENGHAAPVVTSAGALIEGLKTLHAKRIALVAPYMKPLTEMVVDYVENEGVAVADWL
                     ALEIPDNLEVAAQDPARLLDHYKKLDLSGIDALVLSACVQMPSLPSVQRIEDAIGKPV
                     VSAAICTAHQMLSRLGLPTEVPGAGALLSGRY"
     gene            complement(3329..4237)
                     /locus_tag="VN034_06060"
     CDS             complement(3329..4237)
                     /locus_tag="VN034_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWV60196.1"
                     /translation="MRFVTYRTVETEPRLGLLHDGLVIDVEYFGDAIGQDLPSTMLDF
                     IDLGPIGLRFLREAIESATTADLIGTSLPEGNVTLLAPIPRPRKNIFGIGLNYTEHVA
                     ESARSLDTSKELPQQPVIFSKPPTAVVAWNDPIRHNAKVTQQLDWETELAVIIGSTAR
                     GVVEADALNHVFGYTVINDVSARDCRRAGQWIVSKGQDSFAPMGPCIVTADEIGDPHN
                     LNILTHVNGVEKQNSNTRFMLFNINQLIADISSVMTLEPGDVIATGTPAGVGAGRDPQ
                     EFMWPGDVVECTVEGIGTLRNPIVAV"
     gene            complement(4225..5364)
                     /locus_tag="VN034_06065"
     CDS             complement(4225..5364)
                     /locus_tag="VN034_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV60197.1"
                     /translation="MAGNSTIIDPRDDVLGRARVTDTPELEAFYQELAGRNAGAFWKR
                     ANAIEPWEPETRYRPTLWRYADMRDMCLRALDLVKPEEAGRRVVTLLNDSDAGRENVA
                     VCGWLFSGMQAMRPGEITPAHKHTASAHRFIMEGKGAYTVVDGHHITLGANDYVLTPG
                     GGWHDHGVVAEGEVSIWQDGLDIPLMNSLETNFYAVYDQPAQTAAFPVDDLPLSYGGG
                     SLRPEGIGAWDKPYSPVMVYRWAATRDALWNLAKVSEGTPFDGHMVRYSNPLTGGWAL
                     QTMGAHMQMLKPGFRGKAHRHTGNVVYNVAGGRGYSIIGGERFDWQTHDIFCVPAWMW
                     HEHVNLDASEEAFLFSFNDFPVMEALGVRIEEALQENDGHQSCAL"
     gene            complement(5364..6377)
                     /locus_tag="VN034_06070"
     CDS             complement(5364..6377)
                     /locus_tag="VN034_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWV60198.1"
                     /translation="MSDVSAPFLPKSRVAAVQAAPVFLDTARTVDKACALIAEAAGNG
                     AQLVAFPEVFVSAYPYWNWLMTPIEGAEWHERLYRASLLIDGPEVAALCAAARDHGCT
                     VVIGINERDPLSVGTLYNTNLIIGADGSLLGRHRKLVPTWAEKLSWAGGDGSSIRVYD
                     TPVGPLGTLACGENTNTLARFALLSQGELVHVANYIALPVAPASYNMAEAIKIRATAH
                     SFEGKVFTIVACSAVSEEIIAAMSADRSQNRELLSRSNSAFSGVIDPHGNLVGEALID
                     DEGIVYADIDLGECIRPKLMHDIIGGYNRFDIFNLTVDRTPRASALFVDEAEPVDPDE
                     ETP"
     gene            complement(6374..7615)
                     /gene="kynU"
                     /locus_tag="VN034_06075"
     CDS             complement(6374..7615)
                     /gene="kynU"
                     /locus_tag="VN034_06075"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HWV60199.1"
                     /translation="MTETMFATDVAALDAADPLAPFRERFHLREGLIYLDGNSLGALP
                     KATGDRLAEVVGNEWGEGLITSWLGAEWSTAPRRIGDKIGRLIGAKPGEIVATDSTSV
                     NIFKALTAALSLRRERTVILSEATNFPTDVYMMQGIEAFSGGRVKAVTVAPENVVDAL
                     NEDVAVLLLTQVHYKSGRVRDMAAITKRAHEVGALVVWDLSHSAGAIPVDLNGANVDF
                     AIGCGYKFLNGGPGAPAYLFAATRHHGATPVLSGWFGHARPFDFEEDYDPAAGIERFQ
                     CGTPPVLGLSALEVGVDLILEADMAEIRRKSLALGDLFIELMQPLCDAYGFELVSTQG
                     HAERGSQIAYAHPQGYQIVQALKEFDVIADFRAPDILRFGLTPLYLRYKDIVETVERL
                     EKVCATRAWDKPQYHQRAAVT"
     gene            complement(7612..8241)
                     /gene="kynB"
                     /locus_tag="VN034_06080"
     CDS             complement(7612..8241)
                     /gene="kynB"
                     /locus_tag="VN034_06080"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HWV60200.1"
                     /translation="MSRIWDISQPLHAAVPVWPGEPAFALHSHAVIGDGCPVNVGGMF
                     TPLHAGTHGDAPLHYANEGASSADCELDTYIGPCVLLDVRHARSRVEIGDIDWTAIEG
                     TERVLLRTYEHFPHEAWDSDFTAIASDVIARLGAMGVRLIGTDAASLDPEQSKTLDAH
                     QAVKAADMRILEGLVLDDVPPGRYELVALPLRIVGADASPVRAILRKLA"
     gene            complement(8238..9143)
                     /locus_tag="VN034_06085"
     CDS             complement(8238..9143)
                     /locus_tag="VN034_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HWV60201.1"
                     /translation="MIRQTRDGGTDIFETEIDGETIQWDNGLTYARHIQTQQLLSAQV
                     PVSDKPDEMLFIIMHQTMELWLKLVLHEGRIVYEAIKADRLEIAGKGLDRIATIQRHM
                     IHSWEVLATLTPHDFLTFRGFLRRASGFQSQQYRELEYLLGNKREDMMIVHKDDPQAT
                     ERLRATFEAPSLYDELLRLLARRGFDIPADHLERDWTKPYQASEGVEKAWLAIYTDPD
                     RHWDLYTLAEKITALEYYFQEWRFKHMKTVARVIGHKPGTGGSSGVNYLVKALSLSFF
                     PELWSMRTEMVAPREGGDYSEGTRA"
     gene            complement(9140..10012)
                     /gene="phhA"
                     /locus_tag="VN034_06090"
     CDS             complement(9140..10012)
                     /gene="phhA"
                     /locus_tag="VN034_06090"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HWV60202.1"
                     /translation="MAATHIFDAPPEGVAADWTMPQKWSAFTAEQHGIWRTLFDRQSA
                     ALDGYACRSFVDGLGILRKLTPGVPDFAELNALLKPVSGWEVVAVPGWIPNEPFFEHL
                     ANKRFPAANFLRPPEQIAYSEEPDMFHDIFGHVPMLTDPAFSDFLVAYGQAGLRAETL
                     GASDFLGRLWLYTVEFGLVVEDGELRAFGGGLMSSLAETVSALTAPEPRRIWLDIERV
                     MRTGYHFDQLQQTYFVVAGFDELLRATEETDFASIYRKIADEPALEPGDACPGDVAYE
                     GRLPAGPAGGEALL"
     gene            10201..10680
                     /locus_tag="VN034_06095"
     CDS             10201..10680
                     /locus_tag="VN034_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV60203.1"
                     /translation="MDKLDWKIVAALERDGRQSYADLGEQVGLSKSPCWSRVKNLEES
                     GVIEGYAARLDPIAIGLAVQSFVEVQIRFDAHIEFEAAVLAHPAVVECHTTAGESDYL
                     LKIFARSADHLDELLRHNLSKLPGVQRLKTVVCLKTIKRHGHLAEWARTTEGRDRAN"
     gene            complement(10762..14007)
                     /locus_tag="VN034_06100"
     CDS             complement(10762..14007)
                     /locus_tag="VN034_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60204.1"
                     /translation="MRVRACNLRKFLLSGAAIGCLVLSGNVALAREHVTSYRIPAQSL
                     DQALRDFGVQSGATILVDASIVDGKRTAGHSKRSDPETALRALLLGTGLGYRRDGDVF
                     VVTREGNVTPLAAGAEIEDPAEIVVTAQKREEKISDVPIAISAFTPKGLDDRKVESGA
                     ELVRAVPNVNFSKGNFSGYDFTIRGIGTKAVSASSDPAVAVSFNNTPLIRNRLFEAEF
                     FDLERVEVLRGPQGTLYGRNATGGVVNVIPAMPGDEFEAMAKGEVGNYSTRRLSGMIN
                     VPLSDTLAVRAAGAMTKRDGFDYNTFTQQRVNDRDLWSTRVSAQWEPTDDLKASFIWQ
                     HFEEDDQRSRTGKQLCTRDEGPAMLGSTVVPDYLRAKLSQGCKPGSLYSDAAFGAPNA
                     SSFAFVYVAKQIGIGFLPDPVFGIAAVTLIDPDLDPYAGIQQSRNLREISTSYDPVFR
                     AKNDVFQLNLEAGVGDLKLISQTAYSRDRYYSSQDYNRFVSVPIFNDSADPRLIEIGG
                     TGRPNNIPGPTPGGIYTDPQLGPSDRMLAVDVSQSRNSQWTQELRLQSDFSGPFNFSV
                     GANALNFKSQDDYYVFSNLFSVLAEYFYNREEAPGEIGTGTRNCDPGNESRECIYVDP
                     NPIDQIDGQGHNYFRSKNVVQTRSWALFGEAYWNVSDDVKITAGARYTNDKKTSTPIP
                     SQLLLGAENFGNSSGAGSGGRVSIGYPALPPIEQQWDAFTGRLVVDWKPETSFSDDTL
                     VYASFSHGYKGGGSNPPRVDIDPKVIQYQPLAETFKPEYVNAFEIGTKNSFAGGRMTL
                     NATAFFYDYTDYQVSQIVDRIALNENFDAQSWGLELEAAWRPSRNFRIDSNFGYLRTR
                     IGKGAQSIDVMNRTQGNEDWVVLRPQLGVPSNCIAPKDKVEAILNSFFAGPNGIGGFM
                     LSALCAGSSRYGSFNPDVETHLRYDQFMGFTYDPLTDAPNGGRGFYADLEGNELPNSP
                     RFTFNIGAQYTFFIDDWNLTFRGDYYRQAKSYARVYNTEYDRLRAWDNANLAVTLERP
                     ESQFAMQLYVKNLFNDTPITNTFTNSDDTGLSANIFTLDPRIIGFSVTKRF"
     gene            14322..14864
                     /locus_tag="VN034_06105"
     CDS             14322..14864
                     /locus_tag="VN034_06105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWV60205.1"
                     /translation="MPVLSGPVLTMAVPHQDRDLAAWMGTYGPGLRRYFRRRADDADV
                     DDLVQEVFLRLQSAQLSAPIDNVERYLFAAARNALISRHRRQKARATLLHDEFEDGIE
                     IAEQRSPERIVIGQQEYRRVLEAIQNLPPRAREAFQYHRFENLTYQAIAQRMGISKEA
                     VKELMHRALVRIVEEMEPEL"
     gene            14861..15856
                     /locus_tag="VN034_06110"
     CDS             14861..15856
                     /locus_tag="VN034_06110"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWV60206.1"
                     /translation="MSGDDAFARAALRAEAAQWFNLERSGDMTVDDELRFLDWLDQSD
                     AHRDAYRLVERAWLIAGTIPQEAEMLAPPAVEGDVENRRSGWRRHLALAASLLLVFTV
                     GWAVYLDGFRGFGATQDRQFRTGVGQTTTVTLPEGSVVTLDAETEMRLRETPRERRVD
                     LIAGRAFFRVAHDPSRPFTVNAGGKSVRAIGTAFEVSFEHGNMVVTLAEGKVRVEETG
                     PGSGSGTDMAPGGQLVIGADRNWTLSRVDVAKETSWTEGRLIFMRDPLSEAVAEMNRY
                     SPRKLVFKDGKIPEKQVVGVFQAGDVDGFVKAMELNGIARRVSATDDQILLAGSE"
BASE COUNT         3016 a         5174 c         4971 g         2867 t
ORIGIN      
        1 gctcttcccc gaccgccgcg cggtcggggt atccgcgcgt ggaatcatcg tcggcggcgg
       61 cagcttccat tgctcgagcc agcagcttcc gagctagcat cgggcgcgaa gccccctata
      121 ttccgccccg actcccgcac accggatagg ccatgacccg caccatcacc gtcgccgcgc
      181 tgcagcttcc cctgcccggc cccgtcgaac cgaatatcga agccgtctcg gcgctcgtcg
      241 aggaggcggc tgcgaaaggc gcgcaggtca tcctgccgtc cgaactcttc gagggtccct
      301 atttctgcca ggtcgaggaa gaggagttct tcgccaatgc gcggccgacg gccgagcatc
      361 cgagcgtcgt cgcgatgcag gcgctcgcag cgaagctgaa ggtcgcgatc ccgacgagct
      421 atttcgaaaa ggacgggccg cattattaca atacgctcgc gatgatcggt cccgacgggg
      481 caatcatggg aacctatcgc aagagccaca tccccgacgg cccgggctat gaagagaaat
      541 attatttccg cccgggcaac accggcttca aggtctggga cgtgttcggg acgcggattg
      601 gcgtcggcgt gtgctgggac caatggtatc ccgaatgcgc gcgcgcgatg gcgctgatgg
      661 gcgccgaact gcttttctat ccgaccgcga tcgggtccga accatatgac gccgacctcg
      721 acaccagccg catgtggcgc cgcgcgatgc aggggcacag cgtgtcgaac tgcatgccgg
      781 tgatcgccgc aaaccgcatc gggcccgaag gcgacgccaa tttctatggc cacagcttca
      841 tcaccgacga atggggcgac atgacgcagg cgttcggagc gagcgaaacc ggggtccttg
      901 tcgaaacgat cgatctcgac cgcgccgcga aacaccgcgc cggcatgggc tttttccgcg
      961 accgccggcc acagctatac gggcggctcg tcgaggacat ctgatcgcca gcgagggggc
     1021 aagcgatgat attacccgaa gatatcttca ccgagcccga ggatgtcgat cccgaaacgc
     1081 tcgccaacct gggcccgctc cgccggcttg cgggcatatg ggaggggcag cgcggggtcg
     1141 atatcaatcc caaggccgac ggccccgaaa cccgcgaata ttatgaacgg atcgaattgc
     1201 agccgatcga tccgcaggcg aacgggccgc agcttttcta cgggctgcgc taccatctcc
     1261 acgtcaacac gcgcgaggag gacatcgcct tccatgacca ggtcggttac tggctttacg
     1321 aggcgtcgac cggcctgatc ctccagacgc ttgcgatccc gcgcgggcag atcgcgatcg
     1381 ccgcgggtca tgccgaaccc gatgcgaagc aactcatggt caaggcggaa cgcgggcaaa
     1441 gtgaatatgg catctgctcg acgaccttcc tcgaactcgc ctttcgcacc gacgcgtatc
     1501 aactgacgat cgatttccac gacgacgggt cgtggagcta tgtgtccgac acgacattga
     1561 tcgtgaaggg ccgcgacgag cccttcctgc accgcgaccg caacaggctc gtcaagatcg
     1621 ccgaacccga tctcaacccc tgggcgaaga tcgttcgcgg cgccgcctga ggctcaactc
     1681 tcgaagcggc ggatgctttc caagtcggcg cggtcgagcg gctgggtcag ccaaaggccg
     1741 ccgcggccgc cgcgcgacca gcgcacgatg gcgcggcgct tgaccccgcc cgcgagtatg
     1801 aggtcgaatt ccttgcccac ctcgaaatgg ccgtcgtgga cgaagccggc gccgttctgc
     1861 gacagatcga ccagttcgat gtgcgtcgcc gcgccttcgt cggtcaccgc ttcggccttc
     1921 gtgtgcacgg gcaggcgcgg ctggcgatag ccggtggcgc gctgttcggc cgcgagttgc
     1981 ttgagcacgt cggcgacgtc gacttcttga tcgaaggaaa cgccgcatcg gccctcatca
     2041 gaccacacca cttcgccgcg gatcaccacc gtatcggaga gcgcgatctc cagcttttca
     2101 ccgaccgcga tcggaacgtc ggccgcgagc atcatgccgc gatcggaaat attgcggacg
     2161 cgccacaagc ccgcgtcgcc gttgcgcatc accttggcga tccgccagac cgtccgatag
     2221 cgatcgcctt cgcggcgatc gctgctggcg gcgatgtcct gctcctctac cggatccgag
     2281 aagcgacgtt ggttcatctt gtccaccttg tcgaagcagc gtcagataga agcccaagcc
     2341 cctgccaata ctgcaaaata cgcaaaaatc acacaatcac ttactgggat ttatatgaaa
     2401 attcaataat attgcggatt atagttaacg ccatcgatca aagcgcaggc gcgcgaaccg
     2461 gttgctcgcc gccagcgaat acttcgtatt ttacacaatt tacggtgcag ggaaggcggg
     2521 attaagcccc aagaggcgaa gaccgcgaaa tgcctgccga tccgcttctc aatagcggcc
     2581 cgacagcaag gcgccggcgc ccggaacctc ggtcggcaac ccgaggcgcg aaagcatctg
     2641 gtgcgcggtg cagatggcgg cggaaacgac gggcttgcca atcgcatcct cgatccgctg
     2701 aaccgaggga agcgacggca tctggacgca cgccgacagg acgagcgcgt cgatgccgga
     2761 aaggtcgagc ttcttgtaat gatcgagcag cctcgccgga tcctgcgccg caacctccag
     2821 attgtcgggg atttcgagcg cgagccagtc ggcgaccgcg acgccctcat tctcgacata
     2881 gtcgacgacc atctcggtca gcggcttcat atagggcgcg acgagcgcaa tgcgcttcgc
     2941 gtgcaacgtc ttcagcccct cgatcagcgc gccggcgctg gtgacgaccg gcgcggcgtg
     3001 gccattctcg accgtccgct ggtgcagccg cgcttcggac acgcggtgat agccctcgcc
     3061 catgctcatg attgcgacga ggcaggcata gccgagcacg tcgaccgcgg cgtcggacag
     3121 ctcgagcgcg cagcggtcgg aatcggcatc cattgccgcg agctcttctt tcgtcacctt
     3181 cttcattcgc atccgcgacg agtgaaaggt gaagcgttcg ggtgcgatgc cctcgcgcgc
     3241 gcgaagcagc gccggaatct cggtttccat tgtgacgttc gagctgggca cgatctgccc
     3301 gatgcggatg ggccgggtca tcacaccctc aaaccgcgac gatcgggttg cgcagcgtgc
     3361 cgatgccctc gacggtgcat tcgacgacat cgcccggcca cataaattcc tgcggatcgc
     3421 gccccgcacc gaccccggcg ggggtgccgg tcgcgatgac gtccccgggt tcgagcgtca
     3481 tcacgctcga aatgtcggcg atcaactgat tgatattgaa caacatgaag cgcgtattgc
     3541 tgttctgctt ctcgaccccg ttcacatggg tcaggatgtt gaggttgtgc ggatcgccga
     3601 tctcgtcggc ggtgacgata cacggcccca tcggcgcgaa gctgtcctgc cccttcgaga
     3661 cgatccactg ccccgcgcgg cggcagtcgc gcgccgacac atcgttgatg acggtataac
     3721 cgaacacatg gttcagcgcg tcggcttcga cgacgccgcg cgcggtgctg ccgatgatca
     3781 ccgcgagttc ggtttcccaa tcgagctgct gcgtcacctt cgcattgtgg cggatcggat
     3841 cgttccacgc gacgaccgcg gtgggtggct tcgagaagat caccggctgc tgcggcaatt
     3901 ccttcgacgt gtcgagactg cgtgccgatt cagcgacatg ctcggtatag ttgagaccga
     3961 ttccaaagat attcttgcgc ggccgcggga tcggcgcgag cagggtcaca ttgccctcgg
     4021 gcagcgaggt gccgatcagg tcggcggtcg tcgcgctttc gatcgcttcg cgcaggaagc
     4081 gcagaccgat cggccccagg tcgatgaaat cgagcatcgt cgaaggcaaa tcctgcccga
     4141 tcgcgtcgcc aaaatattcg acgtcgatca cgagtccatc gtggagaagg ccaagccgcg
     4201 gttcggtctc gaccgtgcga taagtcacaa agcgcatgat tgatgtccgt cattttcttg
     4261 gagggcttcc tcgatccgga cgccgagcgc ctccatcacg gggaagtcgt tgaacgagaa
     4321 gaggaaggcc tcttcggatg cgtcaagatt gacatgctcg tgccacatcc acgcgggcac
     4381 gcagaaaatg tcgtgcgttt gccagtcgaa acgctcgccg ccgatgatcg aatagccgcg
     4441 cccacccgcg acattataga cgacattgcc ggtgtggcgg tgcgccttgc cgcggaagcc
     4501 gggtttgagc atctgcatat gcgcgcccat cgtctggagc gcccagccgc cggtcagcgg
     4561 gttcgaatag cgcaccatat gcccgtcgaa cggcgtgccc tccgaaacct tggcgaggtt
     4621 ccacagcgcg tcgcgcgtcg ccgcccagcg atagaccatc accggcgaat aaggcttgtc
     4681 ccacgcgccg atgccttcgg gccgcagcga cccgccgcca tagctcagcg gcaagtcatc
     4741 gaccgggaaa gcggcggtct gcgccggctg gtcatagacc gcgtaaaagt tggtctcgag
     4801 gctgttcatc agcgggatgt cgagcccgtc ctgccagatc gacacctcgc cctcggcgac
     4861 gacgccatgg tcgtgccagc cgccgcccgg ggtcagaaca tagtcgttcg cgcccaaagt
     4921 gatgtgatgg ccatcaacga cggtataagc gcccttcccc tccatgatga aacggtgcgc
     4981 cgaggcggtg tgcttgtgcg cgggcgtgat ttcgccgggg cgcatcgcct gcatcccgct
     5041 gaacagccag ccgcacaccg cgacattctc gcgccccgcg tcgctgtcgt tgagcagggt
     5101 cacgacgcgg cgcccggctt cctcgggctt caccagatcg agcgcgcgca ggcacatgtc
     5161 gcgcatgtcg gcatagcgcc agagcgtcgg gcggtagcgc gtctcgggct cccaaggctc
     5221 gatcgcattg gcgcgcttcc agaaggcgcc tgcattgcgt cccgcaagct cttgataaaa
     5281 ggcttcgagt tcgggcgtat cggtgacgcg cgcgcggcca agcacatcgt cgcgcggatc
     5341 aatgatcgtg ctattgcctg ccatcatggc gtctcctcgt cgggatcgac gggctccgcc
     5401 tcgtcgacaa acagggccga cgcgcgggga gtccggtcga ccgtcagatt gaagatgtcg
     5461 aagcggttat agccgccgat gatgtcgtgc atcagcttgg ggcggatgca ctcgccaagg
     5521 tcgatatcgg catagacgat gccctcgtcg tcgatcagcg cctcgccgac cagattgccg
     5581 tgcgggtcga tcacgcccga aaacgcgctg ttcgagcgcg acagcaattc gcgattctgc
     5641 gaccggtcgg cgctcatcgc ggcgatgatc tcctcgctca ccgccgagca ggcgacgatc
     5701 gtgaagacct tgccctcgaa gctgtgcgcg gtagcgcgga tcttgatcgc ctcggccata
     5761 ttgtacgacg ctggcgcgac gggcagcgcg atataattgg cgacgtggac gagctcgccc
     5821 tgcgacagca gcgcaaagcg cgcgagcgtg ttggtgtttt cgccgcacgc gagcgtgccg
     5881 agcggcccga cgggcgtgtc gtagacgcgg atcgagctgc cgtcgccgcc cgcccagctc
     5941 agcttctcgg cccaggtcgg caccagctta cgatgccgtc cgagcaggct gccatcggcg
     6001 ccgatgatca ggttggtgtt gtagagcgtg ccgacgctca acggatcgcg ttcgttgatc
     6061 ccgatgacga cggtgcagcc atggtcgcgc gcggcggcgc agagcgcggc gacctcgggg
     6121 ccatcgatca ggagagacgc gcgatatagg cgctcgtgcc actcggcgcc ctcgatcggc
     6181 gtcatcagcc agttccaata gggataggcg gacacgaaga cctcgggaaa ggcaacgagc
     6241 tgcgcgccat tgccggccgc ctcggcgatc agcgcgcacg ccttgtcgac cgtgcgcgcg
     6301 gtgtcgagga agaccggcgc ggcctgcacc gcggcgacgc gcgacttggg caggaagggt
     6361 gccgacacgt cgctcatgtc accgccgccc gctgatgata ttgcggcttg tcccacgcgc
     6421 gcgtcgcgca gaccttttcg agccgttcga ccgtttcgac gatgtcttta tagcgcaggt
     6481 agagcggcgt cagcccgaag cgcaagatgt cgggcgcgcg gaagtcggcg atgacgtcaa
     6541 attccttgag cgcctgcacg atctggtatc cctgcgggtg cgcataggcg atctggctgc
     6601 cacgctccgc atggccttgt gtgctcacca gttcgaagcc gtaggcgtcg cacaaaggct
     6661 gcataagctc gatgaacaga tcgccgagcg cgagcgactt gcgtcggatt tcggccatgt
     6721 cggcttcgag gatcagatcg acgcccactt ctagcgccga cagacccagc accggcggcg
     6781 tgccgcactg gaagcgttcg atccccgccg cagggtcata atcctcctca aaatcgaacg
     6841 gccgcgcatg gccgaaccag cccgacagca ccggcgtggc gccatggtgg cgcgtcgcgg
     6901 cgaagagata ggcgggcgca ccggggccgc cgttgaggaa tttatagccg cagccgatcg
     6961 cgaaatcgac attcgcgcca ttgaggtcga ccggaatcgc gcccgcactg tggctcagat
     7021 cccagacgac gagcgcgccg acctcatgcg cccgtttggt gatcgccgcc atgtcgcgca
     7081 cgcggcccga cttgtaatga acctgcgtca gcagcagcac cgcgacgtcc tcgttcaacg
     7141 catcgaccac attctcgggc gcgacggtga ccgccttcac ccgcccgccg gaaaacgcct
     7201 cgatgccctg catcatatag acgtcggtcg ggaaattcgt cgcttcggac aggatcaccg
     7261 tccgttcacg ccgcaacgaa agagcggcag tcaatgcctt gaagatattc accgaagtcg
     7321 aatcggtcgc gacaatctct ccgggcttcg caccgatcag ccgcccgatc ttgtcgccga
     7381 tccggcgcgg cgcggtcgac cattcggcgc cgagccacga ggtgatcagc ccctcgcccc
     7441 attcgttacc gacaacctcg gcaagccgat cgcccgtagc cttcggcagc gcaccgagcg
     7501 aattgccgtc gagatagatg agcccctcgc gcaggtgaaa acgctcgcga aaaggcgcaa
     7561 gcggatcggc cgcgtcgagc gccgcgacgt cggtggcgaa catcgtctcg gtcatgcaag
     7621 cttcctcaaa atcgcgcgca ccgggctggc gtcagccccg acgatgcgga gcggcagcgc
     7681 gacgagttcg taccggcccg gcgggacgtc atcgagcacg agcccttcca aaatccgcat
     7741 atcggccgcc ttgaccgcct gatgcgcgtc gagcgtcttc gattgctcgg ggtcgagcga
     7801 ggccgcatcg gttccgatca gccgcacgcc catggcaccg agccgcgcga tcacatcgct
     7861 ggcgatcgcg gtgaaatcgc tatcccaggc ttcgtgggga aagtgctcgt aggtccgcag
     7921 caagacgcgc tccgtaccct cgatcgcggt ccaatcgata tcgccaatct cgacccggct
     7981 ccgcgcatga cgcacgtcga gcagcacaca cggcccgata taggtgtcaa gctcgcagtc
     8041 ggctgacgac gccccctcat tggcgtaatg cagcggcgca tcgccatgcg tccccgcgtg
     8101 cagcggcgtg aacatgccgc cgacattgac cgggcagccg tcaccgatca ccgcatggct
     8161 gtgcagcgcg aacgccggct cgcccggcca taccggcacc gcggcgtgga gcggctgcga
     8221 aatgtcccag atccggctca tgcgcgcgtt ccttcggaat aatccccgcc ttcgcgcggc
     8281 gcgaccatct cggtccgcat cgaccagagt tcggggaaga agctgaggct cagcgccttg
     8341 acgagataat tgacccccga cgagcccccg gtgcccggct tgtgcccgat cacgcgcgcg
     8401 acggtcttca tgtgcttgaa gcgccattcc tggaaataat attcgagcgc ggtgatcttt
     8461 tcggcgagcg tgtagagatc ccaatgccga tcggggtcgg tatagatcgc gagccacgcc
     8521 ttctcgacgc cttcggacgc ctgatagggt ttcgtccagt cgcgctccag atgatcggcg
     8581 ggaatatcga aaccgcgccg cgcgagcagc cgcagcagtt cgtcatacag cgacggcgcc
     8641 tcaaacgtcg cgcgcagcct ttcagtcgct tgcggatcgt ccttgtggac gatcatcata
     8701 tcctcgcgct tgttgccgag cagatattcg agctcgcgat attgctgcga ctggaacccc
     8761 gacgcgcggc gcaggaaacc gcggaaggtc aggaaatcat gcggggtcag cgtcgcgagc
     8821 acttcccagc tgtgaatcat atggcgctgg atcgtcgcaa tgcggtcgag ccccttgccc
     8881 gcgatctcca gccggtcggc cttgatcgcc tcatagacga tccggccctc gtgcagcacc
     8941 agcttcagcc acagctccat cgtctggtgc atgatgatga agagcatctc gtcgggcttg
     9001 tccgacaccg gcacctgcgc cgacagcagc tgctgcgtct ggatatggcg ggcataggtc
     9061 aggccattgt cccactggat cgtctcgccg tcgatctcgg tctcgaaaat atcggtgccg
     9121 ccgtcgcgcg tctgcctgat catagcaatg cctctccacc cgccggaccg gccggcaggc
     9181 gtccttcata ggcgacatcg cccgggcagg catcccccgg ttcgagtgcc ggctcgtcgg
     9241 cgatcttgcg gtagatgctc gcgaagtcgg tttcctcggt cgcgcgcagc aattcgtcga
     9301 accccgcgac aacgaaatag gtctgctgca gttggtcgaa atgatatccg gtccgcatca
     9361 cccgctcgat atcgagccag atccgccgcg gttcgggcgc cgtcagcgcc gacaccgtct
     9421 cggcaaggct cgacatcagc ccgccgccaa aggcgcgaag ctcgccatcc tcgacgacga
     9481 gcccgaactc gaccgtgtaa agccacagcc gcccgaggaa atccgacgcg ccgagcgtct
     9541 ccgcgcgcag ccccgcctga ccataagcga ccagaaagtc cgaaaaggcg ggatcggtga
     9601 gcatcggcac atgcccgaaa atgtcgtgaa acatatcggg ctcttcgctg taggcgatct
     9661 gttcgggcgg gcggaggaaa ttggcggcgg gaaagcgctt gttcgcgaga tgctcgaaaa
     9721 agggctcgtt cgggatccag ccgggcaccg cgacgacttc ccaccccgag acgggtttca
     9781 gaagcgcatt gagctcggca aaatcgggaa cgcccggcgt caacttgcga aggataccga
     9841 gcccatcgac gaacgaccgg cacgcataac catcgagcgc cgccgactgc cggtcgaaca
     9901 gggttcgcca gatgccatgc tgctcggcag taaaggcgct ccacttttgc ggcatcgtcc
     9961 aatcggccgc gacgccttcg ggcggtgcgt cgaaaatatg cgtcgcggcc atcggtctct
    10021 ctccaaacct tcggccgcca gactagcccg cacgcgggcg aacattgtgc ggatttggcg
    10081 gcgaagcggc gtgaaaaatc gccattcgtt ctgcgatatt tctcatttga aggaatatgt
    10141 gcctccatgt tagcttctaa ccgtcattct gaccaaaaag ccgattcaag gacggaacgc
    10201 atggacaagc tcgactggaa gatcgtcgcc gcgctcgaac gcgatggccg ccaatcctac
    10261 gccgacctcg gcgagcaggt cggcctttcg aaatcgccct gctggtcgcg ggtgaaaaac
    10321 ctcgaggaaa gcggcgtgat cgagggctat gccgcgcgtc tcgatccgat cgcgatcggc
    10381 cttgcggtgc agagcttcgt cgaggtccag attcgtttcg acgcgcatat cgaattcgaa
    10441 gccgcggtcc tcgcgcatcc cgccgtcgtc gaatgccaca cgaccgcggg cgaaagcgac
    10501 tatctgctca aaatcttcgc gcgatcggcc gaccatctcg acgagctgct tcgccacaat
    10561 ttatcgaaac tgcccggcgt ccagcgtttg aagacggtgg tgtgcctcaa gacgatcaaa
    10621 cgtcacggcc atctcgccga atgggcgcgc acgaccgagg gccgcgaccg cgccaattga
    10681 tcgctaccgc gactccgatc ctgcgcaaaa aaacgggcgc gttcgaaaac gcacccaagg
    10741 aaggaccctg cgccgtgccg atcagaaacg cttggtcaca ctgaagccga taatacgcgg
    10801 gtcgagcgtg aagatattcg cgctcaatcc ggtgtcgtcg ctgttcgtga aagtgttggt
    10861 tatcggcgtg tcgttgaaca gattcttgac atagagctgc atcgcgaact gcgattccgg
    10921 ccgctccagc gtcaccgcca gattggcgtt gtcccatgcg cgcagccggt catattcggt
    10981 gttgtaaacg cgggcatagc ttttcgcctg acggtaataa tcgccgcgaa acgtgaggtt
    11041 ccagtcgtcg atgaagaagg tgtattgcgc gccgatattg aaggtgaagc gcggcgaatt
    11101 gggcagctca ttcccttcca gatcggcata gaagccgcgt ccgccgttcg gcgcatcggt
    11161 gagcggatcg taagtgaaac ccatgaactg gtcgtagcga agatgggtct cgacatccgg
    11221 attgaagctg ccgtaacgcg acgagcctgc acacagggcg gacagcatga agcccccgat
    11281 cccgttgggc ccggcgaaga aggaattcag gatcgcctcg accttgtcct tgggcgcgat
    11341 gcaattggac gggacgccga gttgcgggcg caggaccacc caatcctcat tcccctgcgt
    11401 cctgttcatc acgtcgatcg actgcgcgcc cttgccgatc cgggtgcgca gatagccgaa
    11461 gttggaatcg atccggaagt tgcgcgacgg ccgccacgcc gcttcgagtt ccagccccca
    11521 gctctgggcg tcgaaatttt cgttgagcgc gatgcggtcg acgatctgcg acacctgata
    11581 atcggtatag tcatagaaaa aggccgtggc gttgagcgtc atcctgccac cggcgaagct
    11641 gttcttcgtg ccgatttcga acgcgttgac atattcgggt ttgaacgtct cggcgagcgg
    11701 ctgatactgg atcaccttgg ggtcgatatc gacgcgcggc gggttcgaac cgccgccctt
    11761 gtacccgtgc gagaaggacg catagaccaa tgtgtcgtcg gaaaaggagg tttcgggctt
    11821 ccagtcgacc accagccggc cggtgaaggc gtcccactgc tgctcgatcg gcggcagcgc
    11881 cggataaccg atactgacac gcccgccact ccctgcgccg ctgctgttgc cgaagttctc
    11941 ggccccgagc aaaagctggc tgggaatcgg cgtcgaggtc tttttgtcgt tggtatagcg
    12001 cgcgccggcg gtgatcttca catcatccga gacgttccaa taggcctcgc cgaacagcgc
    12061 ccacgatcgg gtttgcacga cattcttgct acggaaataa ttatgcccct ggccatcgat
    12121 ctggtcgatg gggttcggat cgacatagat gcattcgcga gattcattgc ccgggtcgca
    12181 attgcgcgtg cccgtaccga tttcgcccgg cgcctcttcg cggttgtaga aatattcggc
    12241 aagaacactg aacagattgc tgaagacgta atagtcgtcc tgcgatttga agttcagcgc
    12301 attggcgccg acactgaaat tgaacggccc cgaaaaatcc gattgcagcc gcaattcctg
    12361 cgtccattgc gagttgcgcg actggctgac atcaactgcc agcatccggt ccgacgggcc
    12421 gagttgcgga tcggtataga tgccccccgg cgtcgggccc gggatattgt tcggccgccc
    12481 cgtccccccg atctcgatga gccgcggatc ggccgaatca ttgaaaatcg gcaccgaaac
    12541 gaagcggtta taatcctgcg acgaataata gcggtcgcgc gaataggcgg tctgcgagat
    12601 cagcttcagg tcgccgaccc ccgcttcgag attgagctgg aacacgtcgt tcttggcccg
    12661 gaaaaccggg tcatagctgg tcgatatctc gcgcaggtta cgcgattgct ggataccggc
    12721 atagggatcg agatcagggt cgatcaacgt cacggcggcg atcccgaata ctggatcggg
    12781 caagaatccg attccgatct gctttgccac atatacaaaa gcgaagctcg acgcattcgg
    12841 cgcgccgaaa gccgcgtcgc tgtaaagcga ccccggtttg cacccttggc tcagcttcgc
    12901 gcgcagataa tccgggacga ccgtcgagcc cagcatcgcc ggcccttcgt cacgcgtgca
    12961 aagctgcttg cccgtgcgcg aacgctggtc gtcctcctca aaatgctgcc agatgaagct
    13021 ggccttcaga tcgtcggtcg gctcccactg cgccgacacg cgcgtcgacc acaggtcgcg
    13081 gtcgttgacg cgttgctggg tgaatgtgtt gtaatcgaag ccgtcgcgct tggtcatcgc
    13141 gcccgcggcg cggaccgcca gcgtgtcgga cagcggaacg ttgatcatcc cgctcagccg
    13201 gcgcgtcgaa taatttccga cctcgccttt cgccatcgcc tcgaactcgt cgccgggcat
    13261 cgccgggatg acgttgacca cgccgccggt ggcgttgcgg ccataaagcg tcccttgcgg
    13321 gccgcgcagc acctcgacgc gctcgaggtc gaagaactcg gcttcgaaca ggcggttacg
    13381 gatgagcggc gtgttgttga agctgaccgc gacggcggga tcgctcgagg cggaaaccgc
    13441 tttggttccg atgccgcgga tcgtgaagtc atagccgctg aaattgccct tcgaaaaatt
    13501 gacgttgggc accgcgcgga ccagttcggc gccgctttcg accttgcggt cgtcgagccc
    13561 cttgggcgtg aaggcgctga tcgcgatcgg cacgtcgctg atcttctcct cgcgcttctg
    13621 cgccgtaacg acgatctccg cagggtcctc gatctcagcg ccggcggcga gtggggttac
    13681 gtttccctcc cgcgtcacca caaagacatc gccgtcgcgg cgataaccaa gcccggtgcc
    13741 gagcaagagc gcgcgcagcg ccgtttcggg gtccgaccgc ttgctatgcc ccgcggtgcg
    13801 cttgccgtcg acgatcgacg cgtcgacgag gatcgtcgcg ccgctttgca cgccgaagtc
    13861 gcgcagcgcc tggtcgagcg actgcgccgg aatgcggtaa ctcgtgacat gttcgcgcgc
    13921 cagcgccaca ttgcccgaca acaccaggca gccgatcgcc gcaccgctca acagaaattt
    13981 gcgcaaattg catgcacgaa cccgcatgtc ccctctccct caccctgtgc tgcgggattc
    14041 atgtcccgct gttccggcgt tcgatgcgac gccattcgga tagacgcccc atttccggac
    14101 atggggggat cgtcgaacaa attatcaaca atttcagggt ccagacccat tatccacacc
    14161 ggggaacggg tcgtcaggaa aatttgtcgc gcagggcgat tttcatcccc cctcttgcga
    14221 ttttcccgcg tctatcctgt tgactcccgc acaggatgga gccgcaatga ccgttttggg
    14281 aatgatgccg ggatgacgcg tagggttggg agcatctcgc aatgcctgtt ttatcggggc
    14341 cagtattgac gatggcggtt ccgcatcagg accgcgatct ggccgcatgg atgggaacct
    14401 atggcccggg gttgcgccgc tatttccgcc gccgcgccga cgatgccgat gtcgacgacc
    14461 tggtgcagga ggttttcctg cgcctgcaat cggcccagct cagcgcgccg atcgacaatg
    14521 tcgaacgcta tctgttcgcc gccgcgcgca atgcgctgat cagtcgccac cgccggcaaa
    14581 aggcgcgcgc gaccttgctg cacgacgagt tcgaagacgg catcgaaatc gccgagcaac
    14641 gctcgcccga acgcatcgtg atcggacagc aggaatatcg ccgcgtgctc gaggcaatcc
    14701 agaaccttcc gccccgcgcg cgcgaggcct ttcaatatca ccggttcgaa aatctgacct
    14761 atcaggcgat cgcccagcgg atgggcattt ccaaggaggc ggtgaaagag ctgatgcacc
    14821 gcgcccttgt gcgcatcgtc gaagaaatgg agcccgagct gtgagcggcg acgacgcctt
    14881 cgcccgcgcc gcgcttcgcg ccgaggcggc gcaatggttc aatctcgaac ggtcgggcga
    14941 catgacggtc gacgacgaac tgcgcttcct cgactggctc gatcagtccg acgcgcatcg
    15001 cgacgcctat cggctggtcg aacgggcgtg gctgatcgcc ggaacgatcc cgcaggaggc
    15061 cgagatgctg gcaccgcccg cggtcgaagg cgacgtagag aaccgacgat cgggctggcg
    15121 ccggcatctg gcgctggcgg cctcgctgct gctcgtcttc acggtcggtt gggcggtcta
    15181 tctcgatggt tttcggggtt tcggcgcgac gcaggatcgg cagttccgca ccggcgtcgg
    15241 ccagacgacg accgtcaccc ttcccgaagg ctcggtcgtg acgctcgacg ccgaaaccga
    15301 gatgcggctg cgcgaaaccc cccgcgagcg ccgggtcgac ctgatcgccg ggcgcgcctt
    15361 ctttcgcgtc gcgcacgatc cgtcgcgccc cttcacggtg aacgccggtg gcaaaagcgt
    15421 gcgcgccatc ggcaccgcct tcgaagtcag cttcgagcat ggcaacatgg tggtcacgct
    15481 ggccgaaggc aaagtgcggg tcgaagaaac cggacccggg tcgggcagcg gcaccgacat
    15541 ggcgcccggc ggccagctcg ttatcggcgc cgaccgcaac tggacgctca gccgggtcga
    15601 cgtcgcgaag gaaacgagtt ggaccgaggg gcgcctgatc ttcatgcgcg atcccttgtc
    15661 cgaagcggtt gccgaaatga accgctattc gccgcgaaag ctcgtcttca aggacggcaa
    15721 gatccccgaa aagcaggtcg tcggcgtgtt ccaggcgggt gatgtcgacg gctttgtaaa
    15781 ggcgatggaa ttgaacggca tcgcgcgccg ggtctcggcg accgacgacc agattctcct
    15841 ggcgggaagc gagtagcgcc ggtcagggcg aagccggcgg aatgcccgtc ggaatcagaa
    15901 acagcggcag gatgcgcgcg atcaggtcgc gcatcgcgtc gtcgggctgg tcgagcatcg
    15961 cgcgcacatg cggcccgatg accgcgtcgc cgaacgcgat caccgccatc atcagcaccg
    16021 ccgcgcgc
//
LOCUS       DATMSU010000239         6180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_79915, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000239 DATMSU010000000
VERSION     DATMSU010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79915"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..160)
                     /locus_tag="VN034_06115"
     CDS             complement(<1..160)
                     /locus_tag="VN034_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HWV60207.1"
                     /translation="MKISFIGLGNMGGGMAANLAKAGHEVRAFDLSEEALARAVEAGC
                     TRAASAAEA"
     gene            complement(157..426)
                     /locus_tag="VN034_06120"
     CDS             complement(157..426)
                     /locus_tag="VN034_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60208.1"
                     /translation="MERALIFGFGLFGFGLLFCWLALLGWRHRNDEQISMLEAGILKV
                     TGEEPLPLTRLDRWIQRFQLVMMTIFGPLMALLGGYGLLSELDIL"
     gene            complement(411..1193)
                     /locus_tag="VN034_06125"
     CDS             complement(411..1193)
                     /locus_tag="VN034_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV60209.1"
                     /translation="MTYETLLVETRGAVTLVTLNRPQALNALNSGVLDDLIAAFAAFE
                     ADASQRCAVLTGSGDKAFAAGADIKEMADKPASDFYLEDFFSKWTSDFVKKVRKPWIA
                     AVNGFALGGGCELAMMADFIIASDKAKFGQPEIKLGVAPGMGGSQRLTRAIGKAKAME
                     MCLTGRMMDAAEAERSGLVARVVEHASLVDEAVKTATTIASMPPMAAMVNKDMVNAAF
                     ETTLDQGLIYERRLFQILAATEDKAEGMAAFIEKREGQWKGR"
     gene            complement(1294..2343)
                     /locus_tag="VN034_06130"
     CDS             complement(1294..2343)
                     /locus_tag="VN034_06130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWV60210.1"
                     /translation="MTDDVLISTDVRVGRISLNRPKAIHALNLAMCEAMIDALLKWRD
                     DDDAVEAVIIDHSEGRGFCAGGDIRMLATSGAKDGKEARQFFHTEYRLNHLLFTYPKP
                     VVAFMDGITMGGGVGISQPAKYRVATEHTRFAMPETGIGLFTDVGGGWYLPRLEGRVG
                     AFLALTGARLDGAECLALGLATHYLPSEKLAEAKARIAVDPDRIGGILGELSVTAPPA
                     AIIQHLDKINRLFASDGYEDILAALEADGGEWAEKELATLHGKSPQTCKVALRQLQEG
                     GEMHDFAAQMTQEYAIGSRVVSMHDFIEGVRALIIDKDNSPKWDPPTPEAVTDDWIDA
                     IFEPLPDEEKWTPLT"
     gene            complement(2340..3485)
                     /locus_tag="VN034_06135"
     CDS             complement(2340..3485)
                     /locus_tag="VN034_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV60211.1"
                     /translation="MTDQFQLTDDQLAIQDMARKFTADRITPFAAEWDETHHYPVDVW
                     KAAGELGFGAIYVAEESGGIGLGRLEAALIMEAMAYGCPATSAYVSIHNMATWMIDRF
                     GGAEVKARFLPELVSMEKIASYCLTEPGSGSDAAALKATAKRDGDHYVLNGTKQFISG
                     AGTNDIYVCMVRTGDEKSKGISCLVVEKGTPGLSFGAPEKKLGWNASPTAQVIFEDCR
                     VPAENLVGAEGDGFRFAMAGLDGGRLNIGACSLGGAQRCLDEAIAYTKDRQQFGQPIA
                     DFQNTQFMLADMATDLEASRALLYMAAAKVTANAPDKSRFSAMAKRLATDNGSKIVND
                     ALQLFGGYGYLRDYPIERFWRDLRVHSILEGTNQVMRMIVGRDLLRQ"
     gene            complement(3488..3883)
                     /locus_tag="VN034_06140"
     CDS             complement(3488..3883)
                     /locus_tag="VN034_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWV60212.1"
                     /translation="MADASRRNHSSASPFEPIYGYSRAVRVGNRIDVAGCAPIEPDGS
                     STPGDAGVQAARCLTIIAEALAALGGSPADVVRTRMYITDPADADLVGRAHGSAFKNV
                     RPASTMLVVPALIRPEWKVEIEAEAIIEK"
     gene            complement(3876..4181)
                     /locus_tag="VN034_06145"
     CDS             complement(3876..4181)
                     /locus_tag="VN034_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="I78 family peptidase inhibitor"
                     /protein_id="HWV60213.1"
                     /translation="MDIRWIAIAAALPLAACASSEAPPESTPPPPEAEMTCNAEAVQS
                     YVGQAVTPDLGAAILKASGARTLRWGPPRSAMTMDYRVDRVNIMYDDASKITQVTCG"
     gene            complement(4207..4725)
                     /locus_tag="VN034_06150"
     CDS             complement(4207..4725)
                     /locus_tag="VN034_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60214.1"
                     /translation="MMRWTMPAALLMLAACSGGQDEETGPEASSDIGPLQKAERPAPA
                     EAPAPDAEPEAPAKAVAGDTVDGKTIPPAIRGRWALKAADCAAKRGTDLTALTIDATN
                     LRFFESHGELARVRESDAGSIVADYKFSGEGEEWDRRMQLDLADGGKTLVRRDSGEGA
                     AAGPMRYTRCAG"
     gene            complement(4797..>6180)
                     /locus_tag="VN034_06155"
     CDS             complement(4797..>6180)
                     /locus_tag="VN034_06155"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWV60215.1"
                     /translation="RAVLDRAVAAAKAAQPAWAATNPQRRARVMFEFKRLVEANMDEL
                     AQMLSAEHGKVIADARGDVQRGLDVVEFACAAPHVLKGDYTIGAGPGIDVYSMRQPIG
                     IGAGITPFNFPAMIPLWMGAIATVVGNAFILKPSERDPSVPVRLSELFLEAGMPEGIF
                     QTVHGDKEMVDAILDHPDIGAVSFVGSSDIAHYVYNRGVANGKRVQAMGGAKNHGIVM
                     PDADLDQVVNDLTGAAFGSAGERCMALPVVVPVGEDTANRLREKLVPAIEALRVGVST
                     DAEAHYGPVVTQAHKEKVEGWIQKCADEGAELVVDGRGFTLQGHEKGFFVGPTLFDHV
                     TPDMESYKEEIFGPVLQIVRAADFETALELPSKHQYGNGVAIFTRNGHAAREFAARVQ
                     VGMVGINVPIPVPVAYHTFGGWKRSAFGDTNQHGMEGVKFWTKVKTITARWPDGAPDG
                     GNAFVIPTMG"
BASE COUNT          991 a         2021 c         2033 g         1135 t
ORIGIN      
        1 ccgcctcggc cgccgacgcg gcgcgcgtgc atcccgcctc gaccgcccgc gcgagcgcct
       61 cttcgctaag atcgaaggcg cgcacctcat gccctgcctt cgcaaggttc gcggccatgc
      121 cgccgcccat attgccgagt ccgatgaatg agattttcat aatatatcca gttccgaaag
      181 gagtccgtac ccgccgagaa gggccattaa cggaccgaag attgtcatca tgacgagctg
      241 gaaacgctgg atccaccggt cgagccgcgt caggggaagc ggctcttcac ccgtcacctt
      301 caatatcccg gcctccaaca tgctgatctg ctcgtcgttg cgatgacgcc aaccgagaag
      361 cgccaaccag cagaacaaca gcccgaagcc aaacaggcca aagccgaaaa tcagcgccct
      421 ttccattggc cttcgcgctt ctcgatgaag gcggccatac cttcggcctt atcctcggtc
      481 gcggcgagaa tctggaacag gcggcgttcg tagatgaggc cctgatcgag cgtcgtttcg
      541 aacgcggcgt tgaccatgtc cttgttcacc atcgcggcca tcggcggcat cgatgcgatc
      601 gtggtcgcgg tctttaccgc ttcatcgacg aggctggcat gctcgacgac gcgcgcgacg
      661 aggcccgagc gctcggcttc ggcggcgtcc atcatccggc cggtgaggca catttccatc
      721 gccttggcct tgccgatcgc gcgggtcagc cgctgcgacc cgcccatgcc cggcgcgacg
      781 ccgagcttga tttcgggctg gccgaacttc gccttgtccg acgcgataat gaagtcggcc
      841 atcatcgcaa gctcgcagcc gccgcccaat gcgaaaccat tcaccgcggc gatccaaggc
      901 ttgcggacct tcttcacgaa atcgctcgtc catttcgaga aaaaatcctc gagatagaaa
      961 tcggacgccg gcttgtcggc catctccttg atgtcggcgc ccgcggcgaa cgccttgtcg
     1021 cccgaaccgg tgaggacggc gcagcgctgg ctggcgtcgg cttcgaaggc agcgaaggcg
     1081 gcgatcaggt cgtcgagcac gcccgaattg agtgcgttca aagcctgcgg gcggttcagt
     1141 gtcaccagcg tgacggcgcc gcgcgtttcg acgaggaggg tttcgtaggt catgcaagca
     1201 atctccggat tttcgtcatg ccgggcttga tccggcctcc cgcttcgcga cgtcgaagaa
     1261 gcgggacccc ggatcaagtc ccgggtgacg aggttaggtg agtggggtcc acttttcctc
     1321 atcgggcagc ggttcaaaaa tcgcgtcgat ccagtcgtcg gtgacggctt cgggggtcgg
     1381 cggatcccat ttggggctgt tgtccttgtc gatgatcagc gcgcgcacgc cttcgatgaa
     1441 gtcgtgcatc gaaacgacgc ggctgccgat cgcatattcc tgcgtcatct gtgcggcgaa
     1501 atcgtgcatc tcgccgcctt cctgcaattg gcgcagcgcg accttgcacg tctggggcga
     1561 cttgccgtgg agcgttgcca gctccttttc ggcccattcg ccgccgtcgg cttcgagcgc
     1621 ggcgaggata tcctcatagc cgtcgctcgc gaacaggcgg ttgatcttgt cgagatgctg
     1681 gatgatcgcg gcgggcggcg cggtgaccga cagttcgccc aaaatgccgc cgatgcggtc
     1741 ggggtcgact gcgatgcgcg cctttgcttc ggcaagcttt tcggacggca gataatgcgt
     1801 cgcgaggcca agcgcgaggc actccgcgcc gtcgagccgc gcgccggtga gcgcaaggaa
     1861 tgccccgacg cggccttcga gccgcggcag gtaccagccg ccgccgacgt cggtgaacag
     1921 gccgatcccc gtctcgggca tcgcgaagcg cgtgtgctcg gtcgcgacgc ggtatttcgc
     1981 cggctgcgaa atcccgaccc cgccgcccat ggtgatgccg tccatgaagg cgacgacggg
     2041 cttggggtaa gtgaagagca ggtggttgag gcgatattcg gtgtggaaga attggcgcgc
     2101 ttccttgcca tccttcgcgc cgcttgtcgc aagcatgcgg atgtcgccgc cggcgcagaa
     2161 gccgcgcccc tcgctatggt cgatgatcac cgcctcaacc gcgtcgtcgt cgtcgcgcca
     2221 tttcagcagc gcatcgatca tcgcttcgca catggcgagg ttgagcgcgt ggatcgcctt
     2281 ggggcgattg agcgagatgc ggccgacgcg aacgtcggtc gagatcaaaa catcgtccgt
     2341 cattggcgca gcaggtccct tccgacgatc atccgcatca cctggttggt gccttcgagg
     2401 atcgaatgga cgcgcaggtc gcgccagaag cgctcgatcg gatagtccct caaataaccg
     2461 tagccgccga acagctggag cgcgtcgttg acgatcttgc tgccattatc ggtcgcgagc
     2521 cgcttcgcca tcgccgaaaa gcgcgacttg tcgggggcgt tcgcggtgac cttggcggcc
     2581 gccatgtaaa ggagcgcgcg cgatgcttcg aggtcggtcg ccatgtcggc gagcatgaac
     2641 tgggtgttct ggaaatcggc gatcggctgc ccgaattgct ggcggtcctt ggtgtaagcg
     2701 atcgcttcgt cgaggcagcg ctgcgcgccg cccagcgagc aagcgccgat gttgagccgg
     2761 ccgccgtcga gccccgccat cgcgaagcgg aagccgtcgc cctcggcccc gaccagattc
     2821 tcggcgggca cgcggcaatc ctcgaagatc acctgcgcgg tcggcgaggc attccagccg
     2881 agcttctttt cgggcgcgcc gaagctcagg cccggcgtgc ccttttcgac gacgagacag
     2941 ctgatgcctt tcgacttctc gtcgccggtg cgaaccatac agacatagat gtcgttagtg
     3001 cccgcgcccg agatgaactg cttggtgccg ttcagcacat aatggtcgcc gtcgcgcttg
     3061 gccgtggcct tgagcgccgc ggcgtccgag cccgaacccg gctcggtcag gcaatagctc
     3121 gcaatcttct ccatgctgac gagctcgggc aggaagcgtg ccttgacctc cgcgccgccg
     3181 aagcggtcga tcatccacgt cgccatattg tggatcgaga cataggcact ggtcgcgggg
     3241 cagccatagg ccatcgcctc catgatcaac gccgcctcga gccggccgag cccgatgccg
     3301 cccgattcct cggccacgta aatggcgccg aagccgagct cgcccgccgc cttccacaca
     3361 tcgacgggat aatgatgcgt ctcgtcccat tcggccgcaa agggcgtgat gcggtcggcg
     3421 gtgaatttgc gcgccatgtc ctgaatggcg agctggtcgt cggtaagctg gaactggtcg
     3481 gtcattgtca tttctcgatg atggcttcgg cttcgatttc gactttccac tcgggccgga
     3541 tcagcgcggg cacaaccagc attgtcgatg ccggacggac atttttgaat gccgatccgt
     3601 gcgcgcggcc gacgaggtcg gcatcggcgg ggtcggtgat atacatgcgc gtgcgcacga
     3661 cgtcggcggg cgacccgccg agcgccgcga gcgcctcggc gatgatcgtc aggcagcgcg
     3721 ccgcctgaac ccccgcatcg ccgggggtcg aactgccgtc gggctcgatc ggcgcgcagc
     3781 ccgcaacgtc gatgcggttc cccacccgaa ccgcgcggct gtagccgtag atcggttcga
     3841 acggggacgc cgaggagtga ttgcgccggg acgcgtcagc cacaggtgac ctgcgtgatc
     3901 ttggacgcgt catcgtacat gatgttcacg cggtcgacgc gatagtccat cgtcatcgcc
     3961 gagcgcggcg ggccccagcg cagcgtgcgc gcgcccgatg ccttgaggat cgcggcgccg
     4021 agatcggggg tgacggcctg gccgacatag ctctgcaccg cttcggcgtt gcacgtcatt
     4081 tcagcctcgg gcggcggcgg ggtcgattcg ggcggcgctt cgctgctcgc gcaggcggcg
     4141 agcggcaggg cggcggcaat tgcgatccag cgaatgtcca tctttcgtct ccctttcaaa
     4201 aagccgtcaa ccggcgcagc gcgtatagcg catcggaccg gcagcggcac cctcgcccga
     4261 atcgcggcga acgagcgtct tgccgccgtc ggcgaggtcg agctgcatcc ggcgatccca
     4321 ttcctcgccc tcgccgctga atttatagtc ggcgacgatg ctgcccgcgt cgctttcccg
     4381 cacgcgcgcg agttcgccgt gcgattcgaa gaagcgcaga ttggtcgcat cgatcgtcag
     4441 cgcggtgagg tcggtgcccc ttttcgcggc gcaatcggcc gccttcagcg cccagcggcc
     4501 gcggatcgcg ggcggaatcg tcttgccgtc gaccgtgtcg cccgccacgg cctttgcagg
     4561 cgcttcgggt tcggcgtcgg gcgcgggcgc ttcggcgggc gccgggcgct cggccttttg
     4621 cagcgggccg atatccgagc tggcttcggg tcccgtttcc tcatcctgtc cgccgctgca
     4681 cgcagcgagc atcaacagcg cggcaggcat cgtccatcgc atcatcgtca ttctccttcg
     4741 cggggagaaa tgttcgaccg ggcacagagg tttcaccgct gcgcccgatc cgggcctcat
     4801 cccatcgtcg gaatgacgaa ggcgttgccg ccatccggcg cgccatcggg ccagcgcgcg
     4861 gtgatggtct tgaccttggt ccagaatttg acgccttcca tgccgtgctg gttggtgtcg
     4921 ccgaacgccg aacgcttcca gccgccgaag gtatgatagg cgacgggcac cgggatcggc
     4981 acgttgatgc cgaccatgcc gacttgcacg cgcgcggcga attcgcgcgc ggcgtggccg
     5041 ttgcgcgtga agatcgcgac gccgttgcca tattggtgct tcgacggcag ttcgagcgcg
     5101 gtttcgaaat cggcggcgcg gacgatctgg agcacggggc cgaagatttc ttccttgtag
     5161 ctttccatgt cgggggtgac atggtcgaac agggtcgggc cgacgaaaaa gcccttttcg
     5221 tgcccctgca gcgtgaagcc gcggccgtcg acgacgagct cggcgccttc gtcggcgcat
     5281 ttctggatcc agccttcgac cttttccttg tgcgcctgcg tcacgacggg gccgtaatgc
     5341 gcctcggcat cggtcgacac gccgacgcgc agcgcctcga tcgcggggac gagcttttcg
     5401 cgcagccggt tcgcggtgtc ctcgccgacg gggacgacga cgggcagcgc catgcagcgt
     5461 tcgcccgccg agccgaaggc ggcgccggtg aggtcgttca cgacctggtc gaggtcggcg
     5521 tcgggcatga cgatgccatg gttcttcgcg ccgcccatcg cctggacgcg ctttccgttg
     5581 gcgacgccgc ggttatagac ataatgcgcg atgtccgacg aaccgacgaa gctgaccgcg
     5641 ccgatgtcgg gatggtcgag gatcgcgtcg accatttcct tgtcgccgtg cacggtctgg
     5701 aaaattccct cgggcatgcc ggcttcgagg aacagctccg acaggcgcac cggcaccgac
     5761 gggtcgcgct cgctgggctt gaggatgaag gcgttgccga cgacggtcgc gatcgcgccc
     5821 atccacagcg ggatcatcgc ggggaagttg aacggcgtga tgccggcgcc gatgccgatc
     5881 ggctggcgca tcgaatagac gtcgatgccg ggaccggcgc cgatcgtata atcgcccttc
     5941 agcacgtgcg gcgcggcgca ggcgaattcg acgacgtcga gcccgcgctg gacgtcgccc
     6001 ctggcatcgg cgatcacctt gccatgctcg gccgacagca tctgcgcgag ctcgtccata
     6061 ttggcttcga cgaggcgctt gaattcgaac atcacgcgcg cgcggcgctg agggttggtc
     6121 gcggcccatg ccggctgggc ggccttcgcc gccgccaccg cgcggtcgag gacggcgcgg
//
LOCUS       DATMSU010000240         1713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_156063,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000240 DATMSU010000000
VERSION     DATMSU010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156063"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VN034_06160"
     CDS             <1..905
                     /locus_tag="VN034_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60216.1"
                     /translation="RRRHPTMPEETPMRFMLLAAALVAVPAAAQGDAPIVVTGVEKQI
                     ESFVGALTQASPRGQIGRFEKAVCPGAFGMPDAQRAAVDARIRTVVAGVGLKVAKANC
                     KPNLVVMVTPDKAAFLEALGKKRYYMFGDRSPAEVRRILAQPGPATAWQIQAMVNGDG
                     RPIFNEAGTPINRSTRAQSRITPPARPAFMAAIVVVESKAIEGLSTTQLADYAAMRAL
                     ARIDPARIDASAPSTILRVLDAAADSEVPVTLTQWDFSFLKGLYSSPSNLYAPSQRSE
                     IGRVMEREMERTDGGKTPPSNTKE"
     gene            950..1393
                     /locus_tag="VN034_06165"
     CDS             950..1393
                     /locus_tag="VN034_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV60217.1"
                     /translation="MRKTCAVAIASMLILTPAQARDAATDLRAFADAFDAAQIAQDRA
                     ALDRMVADDLVFIDGSGKRYGKAFFIDGWTGADDDYDPVTLSDRVILPLGEEAGLASA
                     ETILSGRSAGKPFRVRIRFTDIFKRHGDSWQATYIHVTRMAIERE"
     gene            1465..>1713
                     /locus_tag="VN034_06170"
     CDS             1465..>1713
                     /locus_tag="VN034_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60218.1"
                     /translation="MDPMFRYFLGFQVNADRAGWLARQLPPVSGDLFAGLKPQLYHLT
                     LCTIAETIEPQPFLRGQVAKAFTPGLPAAAPIPFGRIVA"
BASE COUNT          318 a          565 c          528 g          302 t
ORIGIN      
        1 ctcgacgccg ccacccgacc atgccggagg agacgcccat gcgcttcatg cttctcgccg
       61 cggctcttgt cgccgttccc gccgctgcgc agggcgatgc accgatcgtc gtcaccggtg
      121 tcgagaaaca gattgaaagc ttcgtcggtg cgctgaccca ggcgtcgccg cgtggccaga
      181 tcgggcggtt cgaaaaggca gtatgccccg gcgccttcgg gatgcccgac gcccaacgcg
      241 cagccgtcga tgcacgcatc cggaccgtgg tcgcaggcgt cggactcaaa gtggcaaagg
      301 cgaactgcaa acccaatctg gtggtcatgg tcacgccgga caaagccgcc tttctggaag
      361 cgctgggcaa aaaacgctat tatatgttcg gcgaccgatc ccctgccgag gtcaggcgca
      421 ttttggccca gccaggcccg gcgacggcat ggcagattca ggcaatggtg aacggcgacg
      481 gcaggccgat attcaacgaa gcaggcaccc cgatcaatcg ttcgacgcgg gcgcaatcac
      541 gcatcacccc gccggcccgg cccgcgttca tggccgcgat cgtggtcgtg gagagcaagg
      601 ccatcgaggg actctcgacc acccaactcg ccgattatgc ggcgatgcgc gctttggcac
      661 ggatcgaccc ggcgcggatc gacgcgtcgg cgccttcgac gatcctgcgg gtgctcgacg
      721 ccgcggccga cagcgaagtg ccggtcacgc tgacccaatg ggatttcagc tttctgaagg
      781 ggctttattc cagccccagc aatctctacg ccccgtcgca gcgctccgaa attggccgtg
      841 tcatggaacg ggagatggag cgtacggatg gcggcaaaac cccgccttcc aacacgaagg
      901 aatgacgaca ggtcagggcg tgccggccgg ccgcaacaag ggttctaaca tgcgaaaaac
      961 ttgcgcggtc gccatcgcct cgatgctgat cctgaccccg gcccaggcac gcgatgctgc
     1021 cacggacttg cgcgcctttg ccgatgcctt cgacgcggcg cagatcgcgc aggatcgtgc
     1081 cgcgctcgac cgcatggtcg ccgacgatct cgtctttatc gacggcagcg ggaaacggta
     1141 tggaaaggcg tttttcatcg acggctggac cggcgccgac gatgattatg accccgtcac
     1201 gctcagcgat cgcgtgatcc tgccgctcgg cgaagaggcg ggtctcgcga gcgccgagac
     1261 gatcctgtcg ggccgctcgg cgggcaaacc ctttcgcgtc cgcatccgct tcaccgatat
     1321 tttcaagcgt catggtgaca gctggcaggc gacctatatc catgtgacgc gcatggcgat
     1381 cgagcgcgaa tagcgagaag gattcgactc tcactctcga tgagaacata ataggaacat
     1441 tgatccgatt cggaggaatg gacgatggac ccgatgttcc gctatttcct gggttttcag
     1501 gtaaatgccg accgcgccgg ctggctcgcg cgccaattgc cgcccgtttc gggcgatctg
     1561 tttgcggggc tgaagcccca gctttaccat ctgacgctct gtacgatcgc cgagacgatc
     1621 gagccgcaac ctttcctgcg cggtcaggtc gccaaggcgt tcacccccgg tctcccggcc
     1681 gccgccccca tccccttcgg gcgcatcgtc gca
//
LOCUS       DATMSU010000241         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_118130,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000241 DATMSU010000000
VERSION     DATMSU010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118130"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VN034_06175"
     CDS             <1..121
                     /locus_tag="VN034_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HWV60219.1"
                     /translation="EFATETREELLYDKAKLLANGDKWERAIAANLAADAPYR"
     gene            289..900
                     /locus_tag="VN034_06180"
     CDS             289..900
                     /locus_tag="VN034_06180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWV60220.1"
                     /translation="MIQLIAFYLFATIVIASAAMVIFARNPVHSVMWLILAFFNAAGL
                     MLLAGAEFIAMLLVIVYVGAVAVLFLFVVMMLDIDFAELRAGFVRYLPLGALVAIILA
                     AELVFAVGAWSAGGVDLAARAAPVVSDKSNIQQIGELLYTKYIFLFEAAGIVLLVAMI
                     GAIVLTHRKRGGVRIQNISAQNRRRPEEATRLVDPGVGQGMEL"
     gene            1031..1336
                     /gene="nuoK"
                     /locus_tag="VN034_06185"
     CDS             1031..1336
                     /gene="nuoK"
                     /locus_tag="VN034_06185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWV60221.1"
                     /translation="MISVGHYLAVSAVLFTLGVLGIFINRKNIIVILMAIELILLAVN
                     INLVAFSAALGDLVGQVFSMFVLTVAAGEAAIGLAILVIYFRGRGTIAVDDANRMKG"
     gene            1451..3511
                     /gene="nuoL"
                     /locus_tag="VN034_06190"
     CDS             1451..3511
                     /gene="nuoL"
                     /locus_tag="VN034_06190"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWV60222.1"
                     /translation="MIQAIVFLPLLAAIIAGLGQRVIGNVPSKILTTGALFISCALSW
                     PIFLSFVTGSGEASVVPVLHWVSSGALQFNWELRVDTLTAVMLVVITTVSALVHLYSW
                     GYMEEDPDQPRFFAYLSLFTFAMLMLVTANNLVQMFFGWEGVGLASYLLIGFWYKKPS
                     ANAAAIKAFVVNRVGDLGFMLGIFGTFLVFGTVSIPEILAAAPGMAGSTITFMGMRLD
                     TMTILCLLLFIGAMGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVCRLSP
                     MFETSDVAQGVVMFVGAATCFFAATVATTQCDIKRVIAYSTCSQLGYMFFAAGAGAYG
                     AAMFHLFTHAFFKALLFLGAGSVIHAMHHEQDMRYYGNLRKSIPITYWAMMLGTLAIT
                     GVGIAGVAGFAGFYSKDGILEAAFASGGGGVIAFWVGVFVAFLTSFYSWRLVFLTFYG
                     KARWEQSEHIQHAVHGEHEDPSEEHSADDHAHAHHHDAHHGVQGDGTAGYHPHESPLS
                     MLIPLVVLSIGAVFAGMAFHHQFIYPEEGLAFWKGSLAFDAELMHHAHEVPTWVKWTP
                     FTAMAIGLFLAWNSYIRNTSLPARFVAQFKLLHQFLYNKWYFDELYHILFVKPAFAIG
                     RFFWKRGDEGTIDRFGPDGAAALVAGGTRLAVRLQSGYVYGYAFVMLLGLVGLASWAM
                     VNFL"
     gene            3508..>3779
                     /locus_tag="VN034_06195"
     CDS             3508..>3779
                     /locus_tag="VN034_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWV60223.1"
                     /translation="MSGFPILSLMLAVPAIAAIACLFVGDKASRFVALGATLATFVLG
                     CILWANFDIGGAQWQFTERADLFGRFQWALGIDGMALMLIMLSVFL"
BASE COUNT          589 a         1192 c         1166 g          832 t
ORIGIN      
        1 cgaattcgcg accgaaacgc gcgaggaact gctctatgac aaggccaagc tgctcgccaa
       61 tggcgacaaa tgggaacgcg cgatcgcggc gaatcttgcc gccgacgcgc cctatcgata
      121 agggcgcgcg cacatgatcc aacttactaa aaatccgacc atcccgagcg aagtcgaggg
      181 actatgccga gcgaagtcga ggcggcttaa cacgcggttc tcgcttcgct cgaacgagcc
      241 cctcgccttc gctcggggat tcggctttga ggcggcaggg ggcgcggcat gatccaactc
      301 atcgcctttt acctcttcgc gacgatcgtc attgcgtcgg ccgcgatggt gattttcgcc
      361 cgcaacccgg tccacagcgt gatgtggctg atccttgctt tcttcaacgc ggcggggctg
      421 atgctgctcg cgggggccga gttcatcgcg atgctgctcg tcatcgtcta tgtcggcgcg
      481 gtcgcggtgc tgttcctgtt cgtcgtgatg atgctcgaca tcgattttgc cgagctgcgc
      541 gccgggttcg tgcgctatct tccgctcggc gcgctcgttg cgatcattct ggccgctgaa
      601 ctggtgttcg cggtcggtgc gtggagcgcc ggcggggtcg acctcgcggc gcgcgcggcg
      661 ccggtggtca gcgacaagag caatatccag cagatcggcg agctgctcta cacgaagtat
      721 atcttcctgt tcgaggcggc gggcatcgtg cttctcgtcg cgatgatcgg cgcgatcgtg
      781 ctgacgcacc gcaagcgcgg cggcgttcgc atccagaata tttcggcgca gaaccggcgc
      841 cgtcccgaag aggcgacgcg cctcgtcgat ccgggcgtcg ggcaggggat ggaattgtga
      901 ttttgttgca cacccccaac ccgttcgcat cgagcgtagt cgagatgccc atcgttcgtg
      961 catgccttca cggtgtctcg acttcgctcg acacgaacgg aattgagatg accggaaatg
     1021 gagatcaaat gtgatttcgg tcggccatta tctcgccgtt tcggcggtgc tgttcacgct
     1081 cggtgtgctc ggcatcttca tcaaccgcaa gaatatcatc gtcatcctga tggcgatcga
     1141 gctcatcctg ctcgcggtga acatcaatct cgtcgcgttc agcgcggcgc tcggcgatct
     1201 cgtcgggcag gtcttctcga tgttcgtgct gaccgttgcc gcgggcgaag ccgcgatcgg
     1261 tcttgccatt ctcgtcattt acttccgcgg ccgcggcacg attgccgtcg acgatgccaa
     1321 ccggatgaag gggtaaaccg gtgatcaaca ccatcctcgt catcccagcg aaagctggga
     1381 tcgccaacgt ggcaacgccg cttaaaaccg tgagcggtcc cagctttcgc tgggacgacg
     1441 gagctttcgc atgatccagg cgatcgtttt cctgccgctg ctcgcggcaa tcatcgcagg
     1501 tctcggccag cgtgtgatcg gcaatgtgcc gtcgaaaatc ctgaccacgg gcgcgctgtt
     1561 catcagctgc gccttgagct ggccgatctt cctgtcgttc gtgacgggca gcggtgaagc
     1621 gagcgtcgtg cccgtgctgc actgggtttc gtcgggcgcg ctccagttca actgggaatt
     1681 gcgcgtcgac acgctgaccg cggtgatgct cgtcgtgatc acgacggtgt cggcgctcgt
     1741 ccacctctat agctggggct atatggagga agacccggac cagccgcgct tcttcgccta
     1801 tctctcgctc tttaccttcg cgatgctgat gctcgtgacc gcgaacaatc tcgtccagat
     1861 gttcttcggt tgggaaggcg tcggcctcgc atcctatctg ctcatcggtt tctggtacaa
     1921 gaagccgagc gccaatgccg cggcgatcaa ggcgttcgtc gtcaaccgcg tcggcgacct
     1981 gggcttcatg ctcggtatct tcggcacctt cctcgtcttc ggcaccgtct cgatccccga
     2041 gattctggcc gccgcgccgg gcatggcggg cagcaccatc accttcatgg gcatgcgtct
     2101 cgacacgatg acgatcctct gcctgctgct gttcatcggc gcgatgggca agtcggcgca
     2161 gcttggcctg cacacctggc tccccgacgc gatggaaggc ccgaccccgg tgtcggcgct
     2221 gatccacgcc gcgacgatgg tcaccgcggg cgtcttcatg gtctgccgcc tgtcgccgat
     2281 gttcgaaacg tcggacgtcg cgcagggcgt cgtgatgttc gtcggcgccg cgacctgctt
     2341 cttcgccgcg accgtcgcga cgacgcagtg cgacatcaag cgcgtgatcg cctattcgac
     2401 ctgttcgcag ctcggctaca tgttcttcgc cgcgggtgcg ggcgcctatg gcgcggcgat
     2461 gttccacctg ttcacgcacg ccttcttcaa ggctttgctg ttcctcggtg ccggttcggt
     2521 catccatgcg atgcaccatg agcaggatat gcgttattac ggcaatctgc ggaaatcgat
     2581 cccgatcacc tattgggcga tgatgctcgg cacgctggcg atcaccggcg tcggcatcgc
     2641 cggggttgcg ggctttgccg gcttctactc gaaggacggc atcctcgagg cggcttttgc
     2701 gagcggcggc ggcggggtga tcgctttctg ggtcggcgtc ttcgtcgcct tcctcaccag
     2761 cttctattcg tggcgcctcg tgttcctgac cttctatggc aaggcgcggt gggaacagag
     2821 cgagcatatc cagcacgcgg ttcatggcga gcatgaagac ccttccgaag aacattcggc
     2881 cgacgatcat gcgcacgcgc accatcatga cgcgcaccat ggcgttcagg gcgacggcac
     2941 cgcgggctat cacccccacg aaagtccgct ttcgatgctc attccgctgg tcgtgctgtc
     3001 aatcggcgcg gtgttcgcgg gcatggcgtt ccaccaccag ttcatctacc ccgaagaggg
     3061 gctggcgttc tggaagggca gcctcgcgtt cgacgccgag ctgatgcacc atgcgcacga
     3121 agtgccgacc tgggtgaaat ggacgccgtt cacggcgatg gcgatcgggc ttttcctcgc
     3181 gtggaacagc tatatccgca acaccagtct gccggcgcgc ttcgtggcgc agttcaagct
     3241 gctccaccaa ttcctgtaca acaaatggta tttcgacgag ctctaccaca tcctcttcgt
     3301 gaagcccgcc ttcgcgatcg gccgcttctt ctggaagcgc ggggatgaag gcaccatcga
     3361 ccgcttcggt cccgatggcg ccgcggcgct cgtcgctggg gggacccgtc tcgcggtccg
     3421 actgcaatcg ggttatgttt atggatatgc gtttgtgatg ctgctcggtc ttgtcggcct
     3481 cgccagctgg gccatggtga atttcctgtg agcggctttc cgatcctttc gttgatgctg
     3541 gcggtccctg cgatcgccgc catcgcctgc ctgttcgtcg gcgacaaggc gtcgcgcttc
     3601 gtcgctctcg gggcgacgct cgcgaccttc gtgctcggct gcatcctctg ggcgaatttc
     3661 gatatcggcg gcgcacagtg gcagttcacc gaacgcgccg acctgttcgg ccgtttccag
     3721 tgggcgctcg gcatcgacgg catggcgctg atgctcatca tgctcagcgt cttcctgat
//
LOCUS       DATMSU010000242         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_42520, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000242 DATMSU010000000
VERSION     DATMSU010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42520"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VN034_06200"
     CDS             complement(<1..227)
                     /locus_tag="VN034_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWV60224.1"
                     /translation="MTVRVRVIPCLDVADGRVVKGVNFVDLRDAGDPVEAARAYDAAG
                     ADELCFLDISASHQGRGTLLDMVSRTAEVCF"
     gene            complement(292..1065)
                     /gene="hisA"
                     /locus_tag="VN034_06205"
     CDS             complement(292..1065)
                     /gene="hisA"
                     /locus_tag="VN034_06205"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWV60225.1"
                     /translation="MAALTIFPAIDLKRGQVVRLAEGDMARATVYGDDPAAQARLFAD
                     AGATHLHVVDLDGAFAGESVNGAAVESIIAAFPGKVQVGGGIRDRAGVDRWLALGVER
                     VIIGTAALKDPEFVKSAARDLPGRIVVGVDARDGMVATEGWADVSDVRVEDLARRFED
                     AGVAALLFTDVGRDGLLKGCNVEATVALAQAVAIPVIASGGVADLGDIHALRPHVADG
                     IEGVITGRALYDGRLDLAEAIAAGKAPSPLQGRGLGRGE"
     gene            complement(1053..1661)
                     /gene="hisH"
                     /locus_tag="VN034_06210"
     CDS             complement(1053..1661)
                     /gene="hisH"
                     /locus_tag="VN034_06210"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWV60226.1"
                     /translation="MSVIALIDYGAGNLRSVHNALVAAGAENVAVTADPDVVAKADRI
                     VLPGVGAFAACMNGLSAIDGLIEAMERRVRGQGAPFLGICVGMQLLADAGEEHGTHKG
                     LGWIGGTVRAFDPAPGLRVPHMGWNDVVPAFAHPMIAAGEAYYLHGYHFADAAHVAAT
                     SSHGATFTAAVAKDNIVGVQFHPEKSQAYGLATLERFLNWRP"
     gene            complement(1671..2258)
                     /gene="hisB"
                     /locus_tag="VN034_06215"
     CDS             complement(1671..2258)
                     /gene="hisB"
                     /locus_tag="VN034_06215"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWV60227.1"
                     /translation="MRTATVQRTTKETDIAIAVNLDGTGEYSVSTGIGFLDHMVEQLS
                     RHSLIDISMQVKGDLHIDQHHTVEDSALALGEAVAKALGDKRGIARYGEAHAPMDETL
                     TRCVLDISGRPHCVYKSSFSQPRLGEMDTEMFPHWFHSFAQAAGITLHIEMLYGENNH
                     HIAESMYKALARALRQAVEIDPRKGDAIPSTKGVL"
BASE COUNT          373 a          763 c          781 g          362 t
ORIGIN      
        1 atgaagcaga cctcggcggt gcggctgacc atgtcgagca aggtgccgcg gccctggtgg
       61 ctggcgctga tgtcgaggaa gcagagctcg tcggcgcccg ccgcgtcata ggcgcgcgcc
      121 gcctcgacgg ggtcgccggc atcgcgcagg tcgacgaaat tcacgccctt gacgacgcgc
      181 ccgtcggcga cgtcgaggca gggaatgacg cggacgcgga cggtcataaa gccctctccc
      241 cttcagggga gagggttggg agagggggaa agggagcgta caaaagcagc atcattcccc
      301 tctccccagc cctctcccct gaaggggaga gggagccttt cccgccgcga tcgcttcggc
      361 gagatcgagg cggccgtcgt agagcgcgcg gccggtgatg acgccttcga tgccgtcggc
      421 aacgtgcggg cggagcgcgt ggatgtcgcc caagtcggcg acgccgcccg atgcgatcac
      481 gggaatggcg actgcttgtg ccagcgcgac ggtcgcctcg acattgcagc ccttgagcag
      541 cccgtcgcgg ccgacgtcgg tgaacagcag ggcggcgacg cccgcatcct cgaaccgccg
      601 cgcgaggtcc tctacgcgca cgtcggagac gtcggcccag ccctcggtcg cgaccatgcc
      661 atcccgcgcg tcgaccccga cgacgatgcg gccgggcagg tcgcgcgcag ccgatttgac
      721 gaactcgggg tctttcagcg ccgcggtgcc gatgatcacg cgctcgacgc cgagcgcgag
      781 ccagcggtcg accccggcgc gatcacgaat gccgccgccg acttgcacct tgcccggaaa
      841 cgcggcgatg atgctctcga ccgcggcgcc gttgacgctt tccccggcaa aggcgccgtc
      901 gagatcgacg acgtggaggt gcgtcgcacc cgcatcggcg aacagccgcg cctgtgccgc
      961 cggatcgtcg ccatagacgg tcgcgcgcgc catatcgccc tcggcgagcc gcaccacctg
     1021 cccgcgcttg aggtcgatcg cggggaagat ggtcagggcc gccaattgag aaacctttcg
     1081 agcgtcgcaa ggccgtaggc ctggcttttt tcggggtgga actggacgcc gacgatattg
     1141 tccttcgcca ccgcggcggt gaaggtcgcg ccgtggctgc tggtcgcggc gacgtgggcg
     1201 gcatcggcga aatgataacc gtggagataa taggcctcgc ccgccgcgat catggggtgt
     1261 gcgaaggcgg ggacgacgtc gttccagccc atgtgcggga cgcgcaggcc cggcgccggg
     1321 tcgaaggcgc gcacggtgcc accgatccag ccgagcccct tgtgcgtccc atgctcctcg
     1381 cccgcgtcgg cgagcaactg catgccgacg cagataccga ggaagggcgc gccttgcccg
     1441 cgcacccgcc gctccatcgc ctcgatcagt ccgtcgatcg ccgagaggcc gttcatgcac
     1501 gcggcgaagg cgccaacgcc gggcaggacg atgcggtcgg ctttcgccac gacatcagga
     1561 tcggcggtga ccgcgacatt ctcggcgccc gccgcgacga gcgcattatg caccgagcga
     1621 agattgcccg cgccatagtc gatcagggca atgacgctca tcggctggtc ctagagaaca
     1681 cccttggtcg aggggatggc gtcgcccttg cgcgggtcga tctcgaccgc ctgccgcagc
     1741 gcgcgcgcga gcgccttgta catgctttcg gcgatgtgat ggttgttctc gccgtaaagc
     1801 atctcgatgt gcagcgtgat ccccgccgcc tgcgcgaaac tgtggaacca gtgcgggaac
     1861 atctcggtgt ccatctcgcc gaggcggggt tgcgagaagc tggatttata gacgcagtgc
     1921 gggcgccccg aaatgtcgag cacgcagcgc gtcagcgtct cgtccatcgg cgcgtgcgcc
     1981 tcgccatagc gcgcgatacc gcgcttgtcg ccgagcgcct tcgccaccgc ttcgccgagc
     2041 gcgagcgccg aatcctcgac cgtgtgatgc tggtcgatat gaaggtcgcc cttcacctgc
     2101 atcgaaatgt cgatcagcga gtggcgcgac aattgctcga ccatatggtc gaggaaaccg
     2161 atgccggtgg acaccgaata ttcgcccgtt ccgtcgagat tgacggcgat cgcgatatcc
     2221 gtttccttgg tcgtgcgctg gacggtggcg gttcgcatgg cgcacgcctt agagcctca
//
LOCUS       DATMSU010000243         4724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_118205,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000243 DATMSU010000000
VERSION     DATMSU010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118205"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..3148)
                     /locus_tag="VN034_06220"
     CDS             complement(215..3148)
                     /locus_tag="VN034_06220"
                     /inference="COORDINATES: protein motif:HMM:NF044284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV60228.1"
                     /translation="MLFSRLFFLRSKGDDKDWWAEASAALVAFPDEVMALRAGGDALV
                     DQAFSEGRILAGTAMTPAARDLLRKGAAILQQTWDQVRLYENAADPAWSIIGCNLLSA
                     YRGLGDKEGAVRTRDQLAALEGTDPNIVHAIAMMATQEGDDAEVVRLLHSLPESSPRT
                     MMMLSAWTRAEDWQAVLEFGTPERRIDLEDEDRQSFDIMMFRARNASETDFDLDIEVD
                     KLLEMWPLGTGAHIMVADVYRRDAPEKLKRMAEKTLRLFDRDTSFTHRVMFAQLSLMR
                     GEWDDIIAALDGFVATDSSSEPLEWLGLAFANAAPRPRTRAFFASLPNELIETGLYAR
                     LAGAAEHNRGDLRQAVKHLRVAITADPSDLRAYLLLISALERDGKVAEAIQFLEALDE
                     TEVEGSPEDRMRLAGILRRHGFTERSLALGYRVAAANRDRESVAAAYPTLIFFNEALP
                     DAVGNASAIGPDYWFSASGDTASDLSGVIDDEEIIGVSRFATTHPLSKAFAGKTVGDS
                     VTIKSKMGPDRNYSVTEIKHKYVWLLHDIMESHAARFPESTSLVQVRMRENDVQNVLD
                     IVRESEKRGRFITQTYQDFPVPAALVATMAKRSVVPFVEHLTTIGVSLRTCDGTMEER
                     RAAAAKVAAYRGRGAVLDTLTFWFAHRANLLGVLHDYFGKICISRSTVDELLELRGRA
                     ELNVGREYMTMGHDGDQAWRQVHTPEDTAGEIAAINAAIEAAQSTCEILASDGTGDNR
                     LDRAMAEFGAHEISAAYDLARDHGLLLLSDDHDYRRLAAHSNVEDSAWLQITLMVARR
                     DDTASEEDYLRAVGMLAAARHDHVCFDAETLLKLRSDPQFGEYWFKAACEFIGGRKAD
                     PLSHVTVAIGFMAGVWATSLTDSEKGQACSYMLTKLIRFRADWQSLLYVVDNDLRAMA
                     RRSVAGASLASDYLEKWMRGHFFNVRDIRSLERIVQIATQKSLPSAAQEPVQSRRRRR
                     RPLKG"
     gene            complement(3067..4194)
                     /locus_tag="VN034_06225"
     CDS             complement(3067..4194)
                     /locus_tag="VN034_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60229.1"
                     /translation="MASSNIIGIPTDDKVFEENCIPLFAGLLKDPNVKLVGTSGGSQG
                     GLDLIGRRDRDPAQPVGIQCKLKTRGDKLTEAEIRREVGLALAHNPPLTEYYIVTTAR
                     DDPAYDKLALEMSQQQAQAGRRIDIQVWGWDTLQTHIRRDPETLRAFDPDYSASTNRL
                     TTLGEEISQTHDILVERASDILAGIGDMQARLSVVAIDTERGTALEAHLDASVDQYRD
                     LMNNGKPRTALGLLEALQTKLDDKSSAAIRARVRANIGFAHLRLDDEANAAALLGEAF
                     VINPTDPKIRGHRVLALALAGDQKGAIAYAKKFSKTILPMRARQARPIRLLHSTALTP
                     TPTSLCHPNYAARKTCSFHACSSCGARETTRTGGPKLLPRS"
     gene            complement(4483..>4724)
                     /locus_tag="VN034_06230"
     CDS             complement(4483..>4724)
                     /locus_tag="VN034_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60230.1"
                     /translation="GWPGQFNLDFLCFLVLSALWTAWRNGFSAIALLLALIAFFGGAG
                     FLLPYLVFLTVQERGNMPRVLIGARTRRDEPYSGR"
BASE COUNT          966 a         1372 c         1368 g         1018 t
ORIGIN      
        1 atatgatcac tcatatgacc ggtcgtaaga gcactcgctc ccgtcgccgc gatatcctcg
       61 ttcatcaccg ctcctcgcaa agagcggcta tcagcaccgc gcagcagctt cctgcaaggc
      121 ggccttcagg acacgcttac atctccccca agattggtgg ggcgcacgtt atgctctgtt
      181 aagagcgggt ccaccagatc gatttgaata tgctctagcc cttgagtggg cgtcggcgcc
      241 tacgtctgct ctggacgggt tcctgagccg cagagggtag agatttctgc gtagcaattt
      301 gaacaattct ttcgagcgat cgaatatcgc ggacgttgaa gaaatgcccc cgcatccatt
      361 tctcaagata atcactcgcc agcgatgcgc cagccacgct tcgacgagcc attgcgcgca
      421 agtcattgtc gacaacgtac aggaggcttt gccagtccgc cctgaaccga atgagcttcg
      481 tcaacatgta gctgcaagct tggccctttt cgctgtctgt cagcgaagtc gcccagaccc
      541 cggccatgaa gccgatcgcg acggtgacat gcgaaagtgg gtcagccttt cgaccgccga
      601 tgaattcgca ggccgccttg aaccaatact ctccaaactg tgggtcggag cggagcttca
      661 agagtgtttc agcgtcgaag caaacgtgat cgtgacgggc cgctgccagc atgccaaccg
      721 cgcgcagata gtcctcttca ctggcggtat catcgcggcg cgctaccata agtgtgattt
      781 gcagccacgc ggagtcctcg acattgctat gtgccgccag ccggcgatag tcgtggtcgt
      841 cggacagcag aagtagtccg tgatcgcggg caagatcata tgcggcggaa atttcgtgcg
      901 cgccaaattc ggccatagcc cgatctagcc gattgtcccc ggtcccgtcg gacgcaagaa
      961 tttcgcatgt cgactgcgcg gcttctatcg ctgcattgat cgcagcaatc tcgccagccg
     1021 tatcttcagg cgtgtggacc tgacgccacg cctggtcgcc gtcatggccc atcgtcatat
     1081 attcgcggcc gacgtttagt tcagcccggc cgcgcagttc tagcaattcg tcgactgtcg
     1141 aacgagaaat gcagatttta ccgaaatagt cgtgaagcac gccaaggagg tttgcccgat
     1201 gggcaaacca gaaggtcaaa gtgtcgagca ctgcgccgcg accacgatat gcggctactt
     1261 ttgctgcggc cgctcgacgt tcttccatcg ttccatcgca cgttcgcagc gacacgccga
     1321 tcgtggttaa atgctcgacg aacggaacta ccgaccgttt cgccatggtg gcgacaaggg
     1381 cggcgggaac cggaaagtcc tggtaagttt gcgtgatgaa ccgaccacgc ttctctgact
     1441 cgcgaacgat gtccagcacg ttctggacat cgttctcacg catgcgaacc tgaaccagcg
     1501 aggtggactc aggaaagcga gccgcatgtg attccattat gtcgtggagg agccagacat
     1561 atttgtgctt gatctcggtc acgctgtagt tgcgatcagg gcccatcttg cttttgattg
     1621 ttacgctgtc gccgaccgtt tttccggcga acgctttcga aagcgggtga gttgtggcga
     1681 accgcgaaac acctataatc tcttcatcat cgataacgcc ggaaaggtcc gatgcggtgt
     1741 cgccggaagc agaaaaccag taatcaggac cgatcgcgct ggcgttgccg actgcatcgg
     1801 gtagtgcctc gttgaaaaag atgagggtgg gataagctgc ggcgacgctt tcccgatcgc
     1861 ggttggctgc cgcaacccta tagccaagag ccagcgagcg ctctgtgaac ccgtgtcgcc
     1921 gcaagatgcc ggcaaggcgc atcctatcct ctgggctacc ctccacctca gtctcatcga
     1981 gcgcttcgag gaattggatc gcctcggcaa ctttgccatc ccgttcaagc gcgctgatca
     2041 gtagcaaata tgcccgcagg tccgatgggt cagcggtaat tgcgacccgc agatgcttca
     2101 ccgcctggcg caaatcaccg cgattgtgtt cggcggctcc ggcgagacgc gcatagagcc
     2161 cggtttcgat tagctcgttc ggcagactgg caaagaacgc acgagttcga gggcggggag
     2221 cagcgttagc gaacgcgaga ccaagccact caagcggttc gctgctgctg tccgtggcga
     2281 cgaagccgtc aagcgcggcg atgatatcgt cccattctcc ccgcatcagc gagagctgag
     2341 cgaacataac gcggtgcgtg aagctggtat cgcgatcgaa tagccgtagc gtcttttccg
     2401 ccattcgctt cagtttctcg ggcgcatcgc gacggtaaac gtcagcgacc ataatatggg
     2461 caccggtgcc gagcggccac atttcgagaa gcttatcgac ttcgatatct agatcgaagt
     2521 cagtctcgga cgcgttccga gcgcgaaaca tcatgatatc gaaggactga cggtcttcgt
     2581 cctcaagatc gattcgccgc tcgggggtcc caaactcgag tacagcttgc cagtcttcgg
     2641 cgcgcgtcca cgccgagagc atcatcattg ttcgcgggga actttcgggc agtgaatgaa
     2701 gtaggcgaac cacttcggca tcgtcgcctt cctgcgttgc catcatcgcg attgcatgga
     2761 cgatattggg atcggtgccc tctagggccg cgagctggtc gcgcgtcctg actgcgcctt
     2821 ctttgtcgcc caatccacga taagcggata aaaggttgca gccgatgatc gaccaagccg
     2881 gatcggccgc attctcgtag agacggacct ggtcccacgt ctgctgaagg attgccgctc
     2941 ctttgcgtaa gaggtctcgc gctgccggtg tcatcgccgt tccggcgagg atccggcctt
     3001 cgctgaaggc ttgatcgacc agtgcgtcgc cgcctgcgcg caacgccatc acttcatcgg
     3061 ggaacgctac gagcgcggca gaagcttcgg cccaccagtc cttgtcgtct cccttgctcc
     3121 gcaggaagaa caggcgtgaa aagagcacgt ctttctcgcc gcgtaattcg ggtggcacaa
     3181 actcgtcggg gttggcgtca acgccgtcga atgaagcaac ctgataggcc gcgcctgccg
     3241 cgcccgcatt ggcaggattg tcttcgagaa cttcttcgcg tacgcgattg cgcctttctg
     3301 atctccggcc agagcgagtg ccaatactcg atgcccccgg atcttgggat cagtggggtt
     3361 aatcacaaac gcttcgccga gaagggctgc tgcatttgct tcatcatcta gacgcaggtg
     3421 tgcgaagcct atgttcgcac gaacccgagc tcttattgcg gctgaactct tgtcgtcgag
     3481 ttttgtttgg agcgcttcga gcaagcccaa cgctgtacgg ggcttgccat tgttcatcag
     3541 gtctcgatat tggtcgacac ttgcgtcgag gtgggcttcg agcgccgtgc cgcgttccgt
     3601 atcgatcgcg acgacgctga gccttgcttg catgtcgccg ataccggcca gaatatccga
     3661 agcccgctcg acgagtatgt cgtgcgtctg ggaaatttct tcgccaagcg tcgtaaggcg
     3721 gttggttgaa gcactatagt caggatcgaa ggcgcgcagc gtttctggat cgcgccggat
     3781 atgcgtttgg agcgtgtccc agccccaaac ctgaatgtcg atgcgccggc ccgcctgcgc
     3841 ttgctgttgc gacatttcca gcgcgagttt gtcatatgcc ggatcgtcac gcgcagttgt
     3901 gacgatgtaa tattcggtga gtggaggatt atgcgcaaga gccaggccga cctcgcgcct
     3961 gatttcggct tcggtgagct tgtcaccacg ggttttcaat ttgcactgta tgccgacggg
     4021 ctgggcgggg tcgcggtcgc gccgcccaat taggtccaac ccgccttgag agccgccgct
     4081 tgtcccgacc agcttaacgt tcgggtcttt gagaagaccg gcgaacagcg ggatacaatt
     4141 ctcttcgaag actttgtcat cggttggaat tccgatgata tttgatgaag ccataaacgc
     4201 accggttccc ttctcatggg gccctttgag cccctgaaac cgaggcggtt tgaatactta
     4261 tgcatatggc ccttctccag cttcgctaca ataggtgagc tgctggttca cattggtgaa
     4321 catacatgaa ggatcatggg aaattacggc cgcgctggcg gaatggaaac gtatccaatg
     4381 tctgcttcca cgtcgcaacg cctcgaagcg gacagactgc agacggccaa gcggcgccgc
     4441 gagaagcgca gttcgttcga tgacggcgaa atgccgtcgc gctcatcgtc cgctgtaagg
     4501 ctcatctctt cgggttcgcg cgccgatcag cacccggggc atgtttcctc gttcctgaac
     4561 tgttagaaag acgaggtagg gaagcaggaa acccgcgcct ccgaaaaagg cgatgagcgc
     4621 gaggagcagg gcgatcgcac tgaatccatt ccgccaagcc gtccaaagcg cagagaggac
     4681 gagaaagcag agaaaatcga ggttgaactg cccgggccat ccca
//
LOCUS       DATMSU010000244        37315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_127893,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000244 DATMSU010000000
VERSION     DATMSU010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 37315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37315
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127893"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VN034_06235"
     CDS             complement(<1..815)
                     /locus_tag="VN034_06235"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWV60231.1"
                     /translation="MPHIRIIAALSACAFMAPNPAIADPGQPVRRVALLDFTAPAAAP
                     PPVPEPAPIEKGMLLDFRQMRSDAPVFAASVPAAVSPVTDIVRSIRVPGWMRSGQSAN
                     QLASLALDLPAIGNCSPKPYLSSGLLGRTAEDRRRVLYPLVQRAACEAGLPIGLMDAM
                     LIQESRYNPMALSPKGAFGLGQLMPGTAKQLGVDRYSLHGNLRGAARYLSWQLREFGR
                     VDLALAAYNAGPGRVRSVKRVPRITETQNYVAKILTNWRVIESNHTQLVPARAT"
     gene            complement(819..1262)
                     /locus_tag="VN034_06240"
     CDS             complement(819..1262)
                     /locus_tag="VN034_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV60232.1"
                     /translation="METRAIAEVSNFLTLCQREAKRLNLSERQIGVRAKMHHTSVSNY
                     LVEKRVPKASHLLRLSTALDIDPTRAFLAIFVFKSLDAYDAPTTKLLASLIPDLAAAI
                     DNCDLEDELRDWETESLLQKLRELLVDLIGPRWKIRQMFFRSRTG"
     gene            complement(1386..1874)
                     /locus_tag="VN034_06245"
     CDS             complement(1386..1874)
                     /locus_tag="VN034_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60233.1"
                     /translation="MATFVAIVATAVASAATIIASSALKIIVTGSAGSVIRKAAASTM
                     KPPPRVPYNIRVSTQYLNALALGIYNLSIKNQKIFTASALFSYVSENCVKELDALNAK
                     HLLLYAPKDSLKGDIRVSYLLDKPVNDVLIAARYQLSARAGRVVTNLEMHRLAISIVA
                     GQ"
     gene            2031..2369
                     /locus_tag="VN034_06250"
     CDS             2031..2369
                     /locus_tag="VN034_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HWV60234.1"
                     /translation="MQTMSMTMTKSRGLFRKENLPLLGAVAFLAFVVLAGPAFAGTDT
                     TFDTAFTKFTAFLEGSGGKIITIISLALGLVGMASGRFSLGQIALPVGVGIGVGTGVP
                     IVTSTVTALI"
     gene            2429..2713
                     /gene="traL"
                     /locus_tag="VN034_06255"
     CDS             2429..2713
                     /gene="traL"
                     /locus_tag="VN034_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HWV60235.1"
                     /translation="MDRYVIPGRLDEPERIGLWTIDEFVAMAGPFVMGILVQHIIWGI
                     CFGILGWWVLRKAKAGRAGSWLIHMAYWYFPSGVMGIRAVPPSYLRLMAG"
     gene            2717..3316
                     /locus_tag="VN034_06260"
     CDS             2717..3316
                     /locus_tag="VN034_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HWV60236.1"
                     /translation="MEAGFSQAQAQRVLKQRNMLVIASTGLAIVTIFSLIAASARDRE
                     IVLQPVLTRQVELSSSGVSREYLELVTRDASVLMLNRSPSSLDYWLEAVLKIVHPSAY
                     GRIKGDLLKIVNDQRGSSVSQYFALEGLRVDTQGLTSEATGTLNTVVGRQVVGSVKRT
                     FRFEWTYTGVELRLIGFGAVVPNKPDGEVTLSAPAPAPL"
     gene            3328..4119
                     /locus_tag="VN034_06265"
     CDS             3328..4119
                     /locus_tag="VN034_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HWV60237.1"
                     /translation="MPVALGAGGVLISARSIDRLHRIFGVGLIGAALLVSHNAYADET
                     VMVEDNSRVACVASEKDLTRISLIGDEFASVSKVQPTNPLDDFSVVNEPTRGDIYLSV
                     PEGFRPKILSFFGTTKKGYVYKFGCRIEAIEAQQIFVSNPVALAKVETAEEGDVEAPD
                     PDETAVRLIQAMASQEVVPGYRMRRSALVPVQAGDLTVQLVAEYEGLDVVGRIVRIEN
                     SSAAPVEITEARIAPADALAVSIANPKLAPRQATSAYVVTRKQGS"
     gene            4239..5447
                     /locus_tag="VN034_06270"
     CDS             4239..5447
                     /locus_tag="VN034_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HWV60238.1"
                     /translation="MLLFGVGGLLLTGAGYWLFFTGNSETPGQANEIASQNIKVSTDD
                     LAARNLSEKEWLSMSENRLTQQDNQIRRMSGQGDQLEQLQAQIDALKSENQNMASEGT
                     RVLSAYDDENRSLKEQLAAAQSAPRPGAGPTALYGAGGPAAYDAVAGAAAAAAQSRRE
                     LKVISFAATPASTGGAAKAPTGGNMFVSDSPNYLPANSYAKARVIVGVDAASNVQSQS
                     DPLPVVLRIVGPARSVAQNGKVLTTRIDGCLVNGAARGDLSSEKVYVKLAKMTCPQPG
                     GRYAESEVKGFIAFGGKTGVRGRVVSREGSLTMQAFFAGLVGGIGRGFSANSTSFLSG
                     ATTIVDGKREKLSTGDIAKGGIGEGVSQAGDMLSQYLIERAEQYQPVIEMPTGVEVEI
                     VFLDGAFVRN"
     gene            5489..6286
                     /locus_tag="VN034_06275"
     CDS             5489..6286
                     /locus_tag="VN034_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HWV60239.1"
                     /translation="MVAFGGAATAVYAADRLEPTRVSALLKARLPKTQVSAIDCDKVA
                     GLCEVVAGKTLFYVDGSARYLIVGRVYDMESRQDLTAAKLLEMNPDMLLGGAAKAGVE
                     EEETPVPQLANAAGPSRAAPPPAAAQKVDLSKLPATGAIVWGNPSGTPVTIFTDLHCG
                     FCRALANELEQMNVRVIERPISTLGTRDLSNRVYCAKDKPRALRAAYAGAEVAPASCD
                     TSGLDANENFARSHGFSGTPVLVRADGTVLEGYRPRAVLETWLKGAK"
     gene            6286..6540
                     /locus_tag="VN034_06280"
     CDS             6286..6540
                     /locus_tag="VN034_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60240.1"
                     /translation="MERIDRVEAGLFGLFFVGLAGAAVVASAGLIEFGAAGRYALLFA
                     LIKSLSICMMLSLGATFGGEALFSLRLKRRRTLAINSNRS"
     gene            6545..7180
                     /locus_tag="VN034_06285"
     CDS             6545..7180
                     /locus_tag="VN034_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60241.1"
                     /translation="MRASPLLILPAALALAGCASMGGNVKGNFICRAPDGICSPTSNI
                     DDQAIASMMGGAAAGAAAPIAANAPAAHSAASLKVVLPARTDRFGRWRDETVVYVEPQ
                     LAAGHSADRQLAGPGAAIPARLSLVELAAGAPAPAELASVSQPTSAKPITGEVFRAEV
                     ERRLSSTRKGPESVDVPPSDAETAAAIAATAQGDTVVTAPGLGGQPAPDGN"
     gene            7180..9756
                     /gene="traC"
                     /locus_tag="VN034_06290"
     CDS             7180..9756
                     /gene="traC"
                     /locus_tag="VN034_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HWV60242.1"
                     /translation="MAGWLDKFLGDLLGGPRKVDKALPSTAIPMFADWLPYRSFDPKT
                     GLYYNSASRGFILEVSPLVGADDQTSEIIAQFLSEGIPDKTSIQILQWMSPRIADRMV
                     QWFVPRQMAKGVYERIAQHRADYLEQGVWSSLSSDAPFHLRSHRVFISLGVPEGAKVS
                     DEELIALRESMSSMLRSIDVESQILDPVGLLKLIDDLTSPTTVSGEDVVDYNRFDPIA
                     DQAVRRDMEIVTDPNRILVRTERFRALGALQDGTPEIGEVVPDRFDFRCYAIRNLPPR
                     WAPWDTVRLIGDPYIDKLRMPCPIATLLTVYYPDEQAATAKAGFKFMRTTSLANSQSA
                     KFTPQVRDQSREWEYVQDQLRQGQKLVRVFYGVMSVSPYGEGDRNERNLKAVYKAAGW
                     ELQDERYLQMAGLLAVMPLTMANGLARDFERLKRFRTMLTTTVANIAPLQGEYNGGPV
                     PHMLLLGRRGQPFFWSPFENTAGNHNVAVFGKSGSGKSVFLQEMTSALVGAGAKVIVI
                     DDGRSFEHSSKLQNGEFVEFTMSSGFCLNPFSMIDEEEAKRDEDYRLDCIAMLKAIIG
                     QMGRHIDRLDDTERGLIDAAVNAEWEANGIRGSIDGVISSLKDTGHVQGEALAIAMRP
                     FADGGTYGRFFKGQSTLKIGASLTVFELSDLSAREELRSVVLAAIMFLSAQAMRKDRN
                     TRKALLLDEAWQLLKGGSMADFVETYARTCRKYGSSLITATQSLNDYYKSEGSIAALE
                     NSDWFVILQQKPETIADFKKLNRFDMSDAVEAMMRSLKRNGTEFSDVMIKGPEMLAIG
                     RLVLDPYSSTLYSSSPQVFASIDRKVAAGLTMAEAIEEVAFPGRENARGGRTEGYAEA
                     AE"
     gene            9753..10121
                     /locus_tag="VN034_06295"
     CDS             9753..10121
                     /locus_tag="VN034_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60243.1"
                     /translation="MTVSAAPLPDRARAVDIRPRKLLQDLAYSLVSVTCWLAINCLAA
                     AGLILGLFVLMANASVDQFFVEAANLSNHYVAADELRRSEFAEVLLYLFGGCVLFFCI
                     TRRGALMADKASIQGEISND"
     gene            10114..10701
                     /locus_tag="VN034_06300"
     CDS             10114..10701
                     /locus_tag="VN034_06300"
                     /inference="COORDINATES: protein motif:HMM:NF021213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI F-type domain-containing protein"
                     /protein_id="HWV60244.1"
                     /translation="MTEQLVLKVGDEPLVPATDATAPAARRGGFAGFTWGQLATGTAL
                     IAALVWGGWTTRELLILKERRIVSISLAGMANDFIMAEARSGASAEQVDADTRHFMSA
                     MQKTLQDRAAAGETIVVSEAVVAASVPDITPEVRSAVGEFIKRNPPPRVGGMTGAPAM
                     PGLAPTLPPAAQPMQAPAPAPVFGGGEGLLPGGGQ"
     gene            10781..11254
                     /locus_tag="VN034_06305"
     CDS             10781..11254
                     /locus_tag="VN034_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HWV60245.1"
                     /translation="MSLDNFAQTHGFLINRTPSLPNWAYFFERGKRVERGEIAFFMPP
                     SNSLVRAHFGAEPPAFGKIAYGMPGDKISHRGSDVILSWEAGPHGDAPSERVVGKLKP
                     LSSRGEKLEAGPTGVIPDGCYYMGSPHPDGFDSRYAAIGFVCRRQILGVSKWSLL"
     gene            11239..11886
                     /gene="traW"
                     /locus_tag="VN034_06310"
     CDS             11239..11886
                     /gene="traW"
                     /locus_tag="VN034_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HWV60246.1"
                     /translation="MVTPVRRRLVLLGAVVCSAFAPLAAAKDYGQQGAVFRITEPDLL
                     RTIEQKLKTLEASGGIERMNAELTRRTEAGVRRPRPVAGIALAVRARSWIFDPAMVVE
                     QDIYDNKGNAIARAGQRVNPMDFIAIRQKLVFIDGDDRDQVNWALRRFDDQSAKLIMI
                     KGAPLDAMTQHQRRFYFDQGGFLVGRFGIRAVPAVVEPEGKAMRVSEVPLGVGRK"
     gene            11919..12941
                     /gene="traU"
                     /locus_tag="VN034_06315"
     CDS             11919..12941
                     /gene="traU"
                     /locus_tag="VN034_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HWV60247.1"
                     /translation="MSFMLLLCALMAVSASAPPARAQISSGIEGTPGKCTGKFVNPVT
                     DVCWSCLFPLSVGGLKIFPSDRADTDNPDLPICACGSPLPRIGVAMGFWEPVRLADVT
                     MKPWCFASLGGKKISPGFNIGHGRAADSVDGHDRGAKWHVHWYVYPLLYWMEILTDVA
                     CLEQSSFDIAYVTEIDPLWQDDALTALINPEVALFANPLAQTACSADCGAATLKLPLD
                     PLFWCAGCLGSMYPMNGNISEHIGHVQSSRLALSRFAFKLHRQGIAWGTMGKKGLCGK
                     YIMPIMRKQQYRFQATVPSPMVKGRWACPPIGASDMKPGSGNTYPAVGEDMGYLVWRK
                     RNCCVL"
     gene            12968..13696
                     /gene="trbC"
                     /locus_tag="VN034_06320"
     CDS             12968..13696
                     /gene="trbC"
                     /locus_tag="VN034_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HWV60248.1"
                     /translation="MAAAAAFSVIGGVAVLAQTVDGLDLQAVLSRGEAADPDAEALVR
                     EVARRGEEMREEAKSAADGGNSNLRDNKGILGGGPAGPIDFDQMLQASNDLEASDKGA
                     PQLIVFASLSMPEQSLKKLIRDTAKAGGTVVFNGFPGNSMKAFQQGIMKVVDNEDAYG
                     SIGIDPRLFRAFDVTAVPAIVVVTSEFELCDGFNCRTSVPPFDRMSGNVPLEYALETF
                     VDGRGPGAPIAGQALARLKGGRGI"
     gene            15952..16842
                     /locus_tag="VN034_06325"
                     /pseudo
     CDS             15952..16842
                     /locus_tag="VN034_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952535.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
     gene            16847..17248
                     /locus_tag="VN034_06330"
     CDS             16847..17248
                     /locus_tag="VN034_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60249.1"
                     /translation="MTDSTPIRAAIDPTPANNPPRSLRAAVAERSDFPDVMIHEWTVL
                     KLCSGDAPLEADEIKYLVDEFFSPPLTMLDLAELVDGLVGKSWLAWDVDNLKLHATAT
                     GREILARIGGSFRLADFSADWVSMAKRRPDR"
     gene            17282..17683
                     /locus_tag="VN034_06335"
     CDS             17282..17683
                     /locus_tag="VN034_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60250.1"
                     /translation="MEKEEFDRLSPEEQKAHLARVERYIMAPGHWPEVSSLQLGVLMR
                     AGGDCPVAVEEVMLSMQDWYGINPEELSHTIKPLIEKEWIAFNEEGFVHTTPLGRAVV
                     EMHKSPLTKAVMWSLKSGISNMEEQDEEGDP"
     gene            17664..18521
                     /locus_tag="VN034_06340"
     CDS             17664..18521
                     /locus_tag="VN034_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HWV60251.1"
                     /translation="MKKAIRSSLALVCAGLVAAGSPLYAQSQPESAESASSPNDRSAD
                     DSEAASGDFYCADRKLGTWFYCEKPKPRPSEKKTPAPSATAASERLASISKELDELKA
                     RAILEPSEENVTAYIRFQREQLDRASMFSDVWQRSMWQNPDLDYTLQRPVTTLAKRAW
                     IDNRKAEQELALRNLSKRYGIFYFYAQSCGACDIFAPILRSLADGSGFNVVAVSMDGG
                     PNRVFPNYVVDVGQHAKMGLSSKATPALVLYDTVRKRPIPIGTGILSADEITERIFTL
                     TSTKAGSDF"
     gene            18521..19969
                     /locus_tag="VN034_06345"
     CDS             18521..19969
                     /locus_tag="VN034_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HWV60252.1"
                     /translation="MAELTFVARIRVAVSRIGIGVIALSVAAAPAQAGVGDEMSEFFN
                     DMGASANATGATAYQGQSAGYYSLGSVWARFPQKSVYPANLQLPKVRAGCGGIDIFTG
                     SFSFINMSEFVANLKAIANNAIGFAFKLAIDSISPQIGGVIDQMQDIANKVNQFNMQS
                     CESAAAAVGAVWPKVDAAEDRICQSIGTSAGRFSDWAKSRRGCGNGGERSSTINSNTD
                     PALAAMTPGPKNYAWDMIKQSPLESADRQMRELVMTLTGTIVVGKRPSDNDPLPIIYR
                     GEGDPAILDALLDGDKPIDVLGCDETDKCLGPGRRTIPALGGTALRPRVAALIQSMSD
                     KVRTNGALTTEEKNLLGVASIPLYKVVAVQAASGFNLSPGELNSLAEVTAIDLLNSIV
                     QRMLDQVAAGQSNNSNQADAENLRQFNEQLARVRQRLFERESTVNQRVTRTFEIVDRA
                     MMIESTLQSRMAPGMAASLNFSRALSAQGLRP"
     gene            19994..23221
                     /locus_tag="VN034_06350"
     CDS             19994..23221
                     /locus_tag="VN034_06350"
                     /inference="COORDINATES: protein motif:HMM:NF019530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HWV60253.1"
                     /translation="MIEVFTVGGGEYLVNVFNAVASWAGSGGYRGLIRVVMVMAFTWA
                     LLVTAWNMDPRALLKWFMQATLMYMVVMVPTISVKVTDRTNPGVTAAVVDNVPVGLGL
                     IAGFTSQIGDYLTRAAETVFAMPQVLNYSTGGMIYGAKLLDATQGLRIDDPVYATNLN
                     EHFKQCVFYDVLLGRKTYDDILKSSDLLAAMGPGSVALSQQYIYPDGTSGIITCQTAY
                     TFISAGWNSYYAVAAPKIAAQFFPGIPAAQATARLNNDMNGMGAAGMASSSGQLVRQA
                     MFINALSEARDGFASGSAQGAIDAFAQTRADIQTRNTYSTIAGGAMKWVPLLNIVLTV
                     VFYSLFPILFLLMLLPTNGMTVAKGYITGFFYLAAWGPLFVILNMIFMSRWQDSLASW
                     GASGGLTAANFSGISALNQDVGALAGYMIMSVPFIAAGMAKGAMAIASHSASFLSPSQ
                     NAAEQAAAEATTGNYAYGNASLMNRQINTLNRDQYSTAPSFNTGAGTIQQRSSDGAFT
                     RYNEDGTFAYDTSQGISNLGFGISASQDFGSQLQKGLSQGSSVVEQKRVAANDSWAAT
                     RTESARLFDTAQTSASATTEEGRSLQSSIAQVQDLSSSWSNTLQTQFGMTKTEADQLA
                     RDTVLTGSAGIGASGALSAGKGGPLSGSADLKAGLDGRSSDTRRTDQGVSTSTQNTDA
                     HQWLEKESNSEAARQARESFYRATTSSTDSQVRGLGQEVSQDLRHSQSLSNEASRAED
                     QYQRWSNEFSQFERDGYALNKNLSQDFVRYAQTAMMDPQNRHLDQSYHPGLINMTPTQ
                     AITQDALVKQFMDDRLEQMHRDLGVVPDAPAATIAGPSFSTADEVRAIGAAGMQEIAA
                     RGPSVDVAGSARDVGLEDNVSGRLGGSVARLDEYGHGMRATLLGDRRQADNLGATAEA
                     RNDSWVIRTMPGVAGALRALGIDGAGEAPLGGQRVNLGGVASAAGLDIKPGANLSSMD
                     SSMAPALTAAAASAHALGLPQRVVTSARDSQHTRGSLHPDGKGVDLRGNDISVAQGKQ
                     WARDVRERLGPDYDVNFETFKDNPANNHLHMEHDPKPRGGRGRKR"
     gene            complement(23257..23478)
                     /locus_tag="VN034_06355"
     CDS             complement(23257..23478)
                     /locus_tag="VN034_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60254.1"
                     /translation="MDDQLKPFGSDWSRRYYDSSHPLYIASAAEDGLARMSHSGGKTA
                     LGPWGRRALYAAFALIASYFALILAVGSH"
     gene            complement(23609..24331)
                     /locus_tag="VN034_06360"
     CDS             complement(23609..24331)
                     /locus_tag="VN034_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HWV60255.1"
                     /translation="MRNGPNAPLDPTTQAYAELQAAFEHYNRMLFDGKLPHCLITMQR
                     EKRTYGYFSRRRFVHTVDQRSTDEIAINPSYFAVVPLLEILQTIVHEMVHAWQAHFGN
                     PSRPGYHNQEWADKMEAIGLMPSSTGEPGGARTGQKMADYPIPGGAFIAATEDLLTSN
                     FKISWCDRFPPEDAAASRPTSSAAALGAGLTGILEPVAGNRSNRIKFRCPVCAAQAWG
                     KPTLLLICGNSDCDLAHLVPTS"
     gene            complement(24574..24777)
                     /locus_tag="VN034_06365"
     CDS             complement(24574..24777)
                     /locus_tag="VN034_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60256.1"
                     /translation="MSGFDFDEVGEFGSQKDADDWARDNNIDPRDVDIKPGSGGKARV
                     WIRRGSTRMSDIELRNSRDRGFL"
     gene            complement(24774..25532)
                     /locus_tag="VN034_06370"
     CDS             complement(24774..25532)
                     /locus_tag="VN034_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60257.1"
                     /translation="MRTMMKALSAAGIAGALATTPIAALAQDSSRTTELYHIFDFKTP
                     MKRAEMIRILQDGINPNISKSNTVTPIVMGPAPEKPGRFTLVNPFENSPYAGFVSTSQ
                     LSTIKQARCDGAVWISSAVRKVRGSQQLMITMCLFPYVEGYQLDVYAIDIKEKGGGLD
                     ARLGRALGQAIVGNSDNWTNKTILDVVRYVRSTAGATVTYVEGQPEFTGTPWEDGIQL
                     APSGADKKTVDTAPAPTGPNTDDRNDPINKESGQ"
     gene            complement(25558..26019)
                     /locus_tag="VN034_06375"
     CDS             complement(25558..26019)
                     /locus_tag="VN034_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HWV60258.1"
                     /translation="MNALIFGQAGAILLVLAGGEMMTSSTAARPLLLEAGTCSAIDGD
                     TLRCGRERVRLLGIDAAERPGHCRQGRVCAPGDPETQRAALQRRMQTAITIMPVKRDR
                     YGRMVAIIHSAAGTNLSCAMLQAGASYVARWDDRQAIRRSCSSTVREANHL"
     gene            complement(26019..26360)
                     /locus_tag="VN034_06380"
     CDS             complement(26019..26360)
                     /locus_tag="VN034_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60259.1"
                     /translation="MSRALAAPIAINRKIAAMSLSTARFITRTGSSRVESVFWVVGAS
                     TAAFGAAIIIASKLGDVAGILTLQRWRSTADALELGGDLLALYLVGHIFLGLVKAVRE
                     EIRWIRRGGDR"
     gene            complement(26405..29653)
                     /gene="mobF"
                     /locus_tag="VN034_06385"
     CDS             complement(26405..29653)
                     /gene="mobF"
                     /locus_tag="VN034_06385"
                     /inference="COORDINATES: protein motif:HMM:NF041492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HWV60260.1"
                     /translation="MTVSIGKVHSAGGAAAYFASDNYYTAEQSEAASAWAGEGAKDLG
                     LSGTVDTATFERILSGELPDGKQIGDAETRDSGRDFTFSMPKSASLLALVSGDKRILA
                     AHMAAVKQTMTWAEKNLAEARVHVDGKDVAVRTGNLVYALFPHDTSRATDPQSHIHAV
                     IANLTRLPERFRNPDQVDQKTGEVTSDDGWRAWHNGAMYRASAVLSSMANAILREKLE
                     GLGYKTEVTGKHGAFEVLGPNGERINKEALDGFSKRGNDIKAKAEELGVHSPEGRRAI
                     TQRTRDPKMDAGDRGELAERWRAEARDYHYNGDKIYAIALSRSQERSSPIERGILAVS
                     SAIRDSRDRLIDYLRRPDDPLVDSGLAKLTRTPATARTQYATASAIRILSEREAVFKT
                     ADIIKTAVDLGEKGVTPDRVERRIAELVEKGEVVPETSQRLDKAVDMITTREAIEQER
                     RILKAMDTGAGEARPLMTADVAATRLAELAAPRELNHQQLAAAIQIVSSPDRIVLVQG
                     RAGAGKSTMLQPIAKAEAIDATARMLSAEGKDALLLTADVKGDAKALAFQNKMVADLK
                     TDTGLEAMTTHAFIFRNEKFLNGEGSAEAFAARKAELAGVYLILDEGSMISNEQMDKL
                     TAIANLMEVGRLAIIGDRKQLNPIDAGKSFSLMQARAIQDQLPVSEVNINMRQKTEEM
                     RLVADLADAGNVRGAIAVLGDRVVESDDRIGDAANAWLALSADEREKTTLLPSSRDGR
                     AEANQIIQDGLKAEGTLQGEGRAFNVREAVQLTREEYRYARNWRQAEFLEVGSRDNGL
                     GLAKGDYRIARIFDNGKVDLVDMRGKKHRVEPIKIDPTGKLNRLKLSNEKRIEVHEGE
                     RIRWTDSDKRDGRGMLNATIASVEKVTADGITVRLASGEVRELPNGDKMLKRMDLAYA
                     INTHMAQGITNETVFSVMGARETNLSNARAFLVNHTRQQNDVRLFTDDKAKLVTQLEN
                     NKGDKSSALEATGELAVEKILSGKGKDFSPAPPVDLDKPSGTSDLDRKLDLSGLADLD
                     VRGSEPKTESKATDLSSPDPSPEKDSGKTPSSPAPPTPREEPQLDKSKGLEL"
     gene            complement(29650..30336)
                     /locus_tag="VN034_06390"
     CDS             complement(29650..30336)
                     /locus_tag="VN034_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60261.1"
                     /translation="MISFFHMEVLEQIEARILEHHSAGRTIHWLGGGADALGLSALDR
                     DAGIAAVFTPTVEIKRQGGRAGGTIAGMSLLFSPPFDLSYVRESKNARVIRAAHIASA
                     RVALRHLESWTLVRDVAGGSLRPSGLIYGLCVADDAFVLDMMVLGWGVGPGMRWGLID
                     GWTLAAKLGSAEDIYASELEDRLASQGLKLTWTGPSHADLKPMLWRDRRARWALAQYG
                     RKNFWQADAS"
     gene            complement(30590..32980)
                     /locus_tag="VN034_06395"
     CDS             complement(30590..32980)
                     /locus_tag="VN034_06395"
                     /inference="COORDINATES: protein motif:HMM:NF021897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HWV60262.1"
                     /translation="MSDKLNRPRHPRAWSSHGEANRNSGNFTRGSQLIGHQFSMWWRG
                     ARVPLLVWIGLLLGFAWLKLTLILDDYEFQMLGTKALAAFWNFVGFDEMKRANVTLRD
                     GSVYQTYMGYVPFIPDVQIAWAKLMNAFWGSLIFSSIGAGMFAWWFIPWARERGESIL
                     ADHHERGVEIVDYDVLKMMLDRHNLDRVRERAKEAFPDLTLAQVEALPLAQRKAAGVI
                     LPYKIATLPFPYGFEQSHVMLTGTTGTGKTTLIRQVVAQAIERGDRCVLFDLTGHYME
                     AFYDPERDFVLNLNDARCESWSLFNDCHSRSELVTAATALIPADHGSDGGFWEKAART
                     LFVEMCDNLRRSGRGTNKHLCDQLMKASLKKIYAELMGTVAEPLVSPDAAKMAQSIRA
                     VLNANAESIRFLPEGGEPFSIKEWIKRDNGEASILFITAQYAHLDMSRSLLTLWMNIA
                     INTIMTLKHTRQLRTWFIFDELAALHQLPALERGLQTARSFGGAFLLGLHNFAGLRNT
                     YGDDGAQNIISLTNTNVILRVKERETAEECSRLIGFRKVRTMDESYSYGAHQTRDAST
                     ISPTTKEEALVIADDITSLPNLNAYIRFPEKFPSTLVSFPYVGFPSIADGFIEAAPPP
                     DFTPVEEDEEEGEGEEGGGQDIAADRPEPGVEAETGRQDRREVFDLPDGRAVRARDVI
                     DVGRPQLDARISAQGETQKPAGITSLAKRSIDDDPPRGVAPRNASTGSSGQQDALANK
                     EQLRGPKDPTEEKSGELARRESRMDFGDTDHRRAGGAELGADGMARGSDDLGMDMD"
     gene            complement(32970..33254)
                     /locus_tag="VN034_06400"
     CDS             complement(32970..33254)
                     /locus_tag="VN034_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HWV60263.1"
                     /translation="MEFHRRSVALSPETWSSLAEIAQHEGITTSELVRRVLNLFLADR
                     DRPQFNPQRMAIICEYMQLVADEWVKKHAPERRDEFLSVVDQRMDRHHVR"
     gene            33985..34350
                     /locus_tag="VN034_06405"
     CDS             33985..34350
                     /locus_tag="VN034_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60264.1"
                     /translation="MLSNLRIDVPITGLDFHALHDSNAVRLDTDRRNEEMPRAKKSEG
                     NLEAITARRAALEAELVELADAERRAREAERDAGRDVFLGALGKVKIGRMDRSEATAI
                     AKAIEKHGAEKIVAKLATI"
     gene            complement(34842..35072)
                     /locus_tag="VN034_06410"
     CDS             complement(34842..35072)
                     /locus_tag="VN034_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60265.1"
                     /translation="MELAINARIAHLENMSRSLNRRIDRTEYFADMIDWHPMTNDDCR
                     LISMQEEHLAETLAETEMELDFRYEQLCWAAH"
     gene            complement(35526..35666)
                     /locus_tag="VN034_06415"
     CDS             complement(35526..35666)
                     /locus_tag="VN034_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60266.1"
                     /translation="MEPDIYEAFSPFVHNDDQFELNFDDPDEQPMFEFAESIPIGSTA
                     PH"
     gene            complement(35719..36078)
                     /locus_tag="VN034_06420"
     CDS             complement(35719..36078)
                     /locus_tag="VN034_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWV60267.1"
                     /translation="MQNCAEFHIIGRVGKVDPKEKVTHLDVAANYPRRDGEGWKDDTH
                     WNRVTCFGKMHEKAAKIGKGDLVRITGRVRQNSYEANGERQYTVDLIVDRIATLVKAG
                     GAPADRDDDDQDDNVPF"
     gene            complement(36518..36985)
                     /locus_tag="VN034_06425"
     CDS             complement(36518..36985)
                     /locus_tag="VN034_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HWV60268.1"
                     /translation="MKPPAASGQPRRQPKQPASTSSWTEPQPTDVISYVYLWRAEKER
                     GLEDGRKTRPCLVLSTYKARGQLRVITAPITTRNYDSGFTVPIPPRVTAHLGLDDRSR
                     VVWNDLNEFAWVGPDVRGTVDGSPFIGIMPEALWRRVIDNVIKLRIDPTHRSE"
     gene            complement(36912..37178)
                     /locus_tag="VN034_06430"
     CDS             complement(36912..37178)
                     /locus_tag="VN034_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWV60269.1"
                     /translation="MELPIEKANSSDVSKRFGFYYDEAMTHAIAIERYGAARVVMLPA
                     SEYERLARLDHIAVTPEELSDEATRSLRTATASAEATRLNELMD"
BASE COUNT         7145 a        11047 c        11526 g         7597 t
ORIGIN      
        1 gttgcccgcg cgggcaccag ctgcgtatga ttggattcaa tcacgcgcca gttggtcagg
       61 atcttggcca catagttctg cgtctcggtg atccgcggaa ctcgcttgac cgaccgaacg
      121 cgaccgggac cggcattata ggccgcaagc gccagatcga cgcgcccgaa ttcgcgaagc
      181 tgccagctca gatagcgggc ggcgccgcgc agattgccgt gaagactgta gcgatcgacg
      241 ccaagctgtt ttgctgtgcc gggcatcaat tgacccaagc cgaaggcgcc ctttgggctg
      301 agtgccatcg gattgtaccg gctctcctgg atcagcatcg catccatcag accgatcggt
      361 aatcccgcct cgcacgccgc gcgctggacg agcggataca gaacgcgcct gcgatcttct
      421 gcggtgcgcc cgagcagacc tgaggagagg tagggcttcg gcgagcaatt cccgatcgct
      481 gggaggtcga gtgccaaact ggccagttgg ttggcggatt ggcccgaacg catccagccc
      541 ggtacgcgga tcgaacgaac aatatccgtg accggcgaga cggctgcagg aacgctggcc
      601 gcaaatactg gtgcgtccga tcgcatctgc cggaaatcta gcagcatgcc cttctcgatc
      661 ggcgcgggct ccggcacggg gggcggggca gcggcaggag ccgtgaagtc gaggagggca
      721 acgcggcgca cgggctgtcc gggatccgcg atcgcggggt tgggggccat gaaggcgcac
      781 gccgatagcg cagcgatgat gcggatgtgc ggcacgcttt atccggtgcg tgatctgaag
      841 aacatttgac ggatcttcca ccggggcccg atcaggtcga ccagcagttc gcgaagtttc
      901 tgcaacagcg attcagtctc ccaatcgcgt agttcgtctt cgaggtcgca attatcgatc
      961 gcggctgcaa gatcggggat gagcgatgcc agcagcttgg tggtgggcgc gtcataggcg
     1021 tccaacgact tgaaaacgaa tattgcaagg aacgcgcgcg tgggatcgat gtcgagggcg
     1081 gtactcaacc gaaggagatg gctcgctttc ggaacgcgtt tttccacaag ataattggag
     1141 acgctggtgt ggtgcatttt tgcacgcacg ccaatttgtc gctcggataa gttcagtcgc
     1201 ttcgcttcac gctgacagag tgtgaggaag ttcgagacct ctgcgatggc ccgtgtttcc
     1261 attttcgccc caatttgctc aattgattat aatataagtg accgcccagc tttgttatgt
     1321 ctggtcaggc aagagcaaag aaggaagaat gtcaagcgca tagcgcttag ctatcgaagg
     1381 cccacttact ggccggcgac gatactgatc gccaaacggt gcatttcgag gttggttacg
     1441 acccgtcccg cgcgtgccga gagctgatag cgcgctgcga tgagcacgtc gttcactggc
     1501 ttatcgagaa gatagcttac cctaatgtcc cccttcaaag aatctttagg agcgtagagc
     1561 aggaggtgtt tcgcgttgag cgcatctaat tctttgacac aattttcaga aacatatgag
     1621 aaaagtgcgg atgcggtgaa aatcttctga ttttttatag agagattgta aattcccagc
     1681 gccagtgcgt tcagatattg ggtactcacc cgaatattat agggaacgcg gggcgggggc
     1741 ttcatggtgc tggctgcggc cttgcggatc acgctaccgg ctgatccggt aacaatgatc
     1801 tttagcgcgc tgctggctat gattgtagcc gcactggcta cggccgtagc cacgatggct
     1861 acaaacgtag ccataaactt tcaacgaatc gtgattaaat cacccgaaat gttcgtagaa
     1921 tgccccaatc tggacctttt attacttaag agttgacagc aagggaagag ggattctccc
     1981 atctcctcgg cgcccattgg ggcgtcgttt caacagatgg gaaatgaacc atgcaaacca
     2041 tgtcaatgac catgaccaag agccgggggc tgttccgcaa ggaaaacctt ccgctcttgg
     2101 gagcggtcgc cttccttgcg ttcgtcgttc tcgcgggtcc ggccttcgct ggcaccgaca
     2161 cgaccttcga caccgcgttc acgaagttca ccgcgttcct cgaaggctcg ggcggcaaga
     2221 tcatcacgat catctcgctc gcgctgggcc tcgtcggcat ggcctcgggc cgcttcagcc
     2281 tgggccagat cgcactcccc gttggcgtcg gcatcggcgt cggcacgggc gttccgatcg
     2341 tcacgtcgac cgtgacggca cttatctgat gtggacggtc cgggtcgcgc accacgcgcg
     2401 cgaatccgga caatagggcg gggcggttat ggatcgctat gtaatcccgg ggagactgga
     2461 cgaacccgaa cgcatcgggc tctggaccat cgacgaattc gtcgcaatgg ccgggccttt
     2521 cgtgatgggt atcctcgttc agcatatcat ttggggcata tgtttcggaa tcttgggttg
     2581 gtgggtcttg cgaaaggcaa aagcgggacg tgctggttct tggcttatcc atatggccta
     2641 ttggtatttc cctagtgggg tgatggggat ccgagccgtt ccgccttctt atcttcgatt
     2701 gatggcgggg tgagcgatgg aagctggatt tagccaggcg caggcgcagc gcgtcctgaa
     2761 gcagcgcaac atgctggtaa tcgcgagcac cgggctcgcc atagtcacga ttttttcttt
     2821 gatcgccgcg tcggcgcggg accgcgagat tgtcttgcag cccgtgctta cccgtcaggt
     2881 cgaactgagc agctcggggg ttagccgcga gtatctcgaa cttgttacgc gagatgcgtc
     2941 ggtcttgatg ttgaaccgca gcccttcgag cctcgactat tggttggaag cggtcctgaa
     3001 gatcgtccat ccgtcggctt acggccgcat caagggtgat cttctcaaga tcgtcaatga
     3061 ccagcgcggt tcgagcgttt cgcaatattt tgcactcgaa ggcctgcgtg tcgacactca
     3121 gggactgacg tcggaggcga cgggcacgct taataccgtg gtcggccgcc aagttgtcgg
     3181 ttcggtcaag cggacgtttc gctttgagtg gacctacacc ggtgtggagc tgcgtctgat
     3241 cggcttcggc gccgtcgtcc cgaataagcc ggatggcgag gtgacgctta gcgcccccgc
     3301 tccggcaccc ctatgatgga ggttctcatg cccgtggcac tcggcgccgg cggtgtgctg
     3361 atttcagcgc gctcgatcga caggctccac cgcattttcg gcgtcggcct gattggcgcc
     3421 gcacttttgg tttcgcacaa tgcctatgcg gacgaaaccg ttatggtcga agataattcc
     3481 cgcgttgcct gcgtggcatc ggagaaggat ctgacccgca tcagtctgat cggcgacgaa
     3541 ttcgccagtg tgtcgaaggt gcagccgacg aacccgctgg acgatttctc agtcgtcaac
     3601 gagccgaccc ggggtgatat ctatttgtcc gtcccggaag gcttccgtcc caagatcttg
     3661 tccttcttcg gcaccacgaa gaagggctat gtctacaaat tcggctgccg gattgaggca
     3721 atcgaggcac agcagatctt cgtgtccaat ccggttgcgc tcgccaaggt cgaaacggcg
     3781 gaggaaggcg acgtcgaggc gcctgacccg gacgagacgg ccgtgcgcct gattcaggcg
     3841 atggcgtcgc aggaggtcgt tcccggttac cgtatgcgcc ggtcggcgct tgtcccggtc
     3901 caggccggcg accttaccgt tcagctcgtc gcggaatacg agggccttga tgtcgtcggc
     3961 cgcatcgtgc ggatcgagaa tagttccgcc gcacccgtcg aaatcacgga agcgcgcatt
     4021 gcgcccgccg acgcacttgc cgtctcgatc gccaatccga agctcgcgcc gcgtcaggcg
     4081 acgtcggcat atgtcgtcac ccgcaagcag ggaagctagc catggtaaat ttgggctcat
     4141 ttttcaaagg tggatcgccc cgcggtgcca ccgctgcccc cgccgttccg gaaggttcca
     4201 gtctttcagt gaacgagggc gttgagaaga ggcagcgtat gctgctcttc ggcgttggcg
     4261 gcctgttgtt gacgggcgct ggctactggc tgttcttcac cggcaactcc gagacgcccg
     4321 gtcaggcgaa tgagatcgcg tcgcagaaca tcaaggtttc gaccgatgat cttgcggcgc
     4381 ggaatctgtc cgagaaagaa tggctgtcaa tgtcggaaaa ccggctgacg cagcaggata
     4441 atcagattcg ccggatgtcg gggcagggcg atcagctcga gcagttgcag gcgcagatcg
     4501 atgcgctcaa atccgagaac cagaatatgg cctcggaggg cactcgcgtc ctgtcagcct
     4561 atgatgacga aaatcgttcg ttgaaggagc aactcgcggc cgcccagtcc gcgccgcgac
     4621 ccggtgcagg cccgactgcg ctgtatggag ctggcggccc cgccgcttat gatgcggttg
     4681 caggtgcggc tgccgctgca gcgcaatcac ggcgcgagct caaggtgatc agctttgcgg
     4741 cgacgccggc gtcgaccggc ggcgcggcca aggcccctac gggcggaaac atgttcgtat
     4801 ccgacagccc gaattacctg cccgcgaaca gctacgcgaa agcgcgggtg atcgttggcg
     4861 tcgatgcagc ttccaacgtg cagtcgcaat ccgatcctct cccggttgtg ttgcgcatcg
     4921 ttggccccgc gcgctcggtt gcgcaaaatg gcaaggtgct gacgacacgg atcgacggat
     4981 gcctcgtcaa cggcgctgcg cgcggggatc tctcttcgga aaaggtctac gtgaagctcg
     5041 cgaagatgac ctgcccgcag cccggtgggc ggtatgccga gagcgaagtt aagggcttta
     5101 tcgcgttcgg ggggaagacg ggtgtgcgag gccgcgtggt cagtcgcgag ggcagtctca
     5161 caatgcaagc cttctttgcc gggcttgtcg gggggatcgg tcgcggcttc tccgcgaatt
     5221 ccacgagctt cctttcggga gcgacgacga tcgtcgacgg caagcgtgag aagctctcga
     5281 ctggggatat cgcgaagggc ggcatcgggg aaggcgtgag ccaggccggc gacatgctgt
     5341 cccagtacct gatcgaacgg gccgaacaat atcagccggt gattgaaatg ccgacgggcg
     5401 tcgaggtcga aatcgtcttc ctcgacggcg cgttcgtgcg caactgatga aagggcgtaa
     5461 gatgaagctc aaggtagcat tgctcgcgat ggtggctttt ggcggcgcgg caactgccgt
     5521 ctacgccgcc gaccgtctcg agccaacccg cgtgtccgct ctcctgaaag cgcggcttcc
     5581 aaagacacag gtctcggcga tcgactgcga caaggtggcg gggctttgtg aagtcgttgc
     5641 gggcaagacg ctgttctacg tcgacggcag cgcgcgctat ctcatcgtcg gccgcgtcta
     5701 cgatatggaa tcgcggcagg atctgacggc ggcgaagctg ctcgagatga accccgatat
     5761 gcttcttggc ggtgcggcca aagcgggcgt cgaggaggaa gaaactccag tgccccagct
     5821 cgccaatgcg gcgggcccga gccgcgcggc gccgcccccg gcggccgcac aaaaggtcga
     5881 cctgtccaag ctccccgcta ctggcgccat cgtgtggggc aatccttcgg gcacccctgt
     5941 gacgatcttc accgatctgc attgcgggtt ctgccgcgcg cttgcgaatg aactggagca
     6001 gatgaacgtg cgcgtgatcg agcgtccgat ttcgacgctt ggcacccgcg atctctcgaa
     6061 tcgcgtttac tgcgcgaagg ataagccccg cgcgcttcgc gccgcctatg ccggcgccga
     6121 agttgccccg gcgagctgcg acacgagcgg cctcgatgcg aatgagaatt tcgcgcgttc
     6181 gcatggcttc agcggcacgc cggttctggt gcgcgccgac ggcactgtgc tggaaggcta
     6241 tcgccctcgc gcagttctgg aaacttggct caagggagcg aagtgatgga gcgcatcgat
     6301 cgcgtagaag cggggctctt cggcctcttc tttgtgggcc tggcaggggc tgcggttgtc
     6361 gccagcgccg gattgatcga atttggtgca gcgggccgtt atgcgttgct ctttgcgctg
     6421 atcaaatcct tgtcgatctg catgatgctg agcctcggcg cgacatttgg cggcgaagcg
     6481 ctgttctccc ttcgcctgaa gcgccgccgc acccttgcca tcaatagcaa caggagctga
     6541 cgacatgcgt gcttcaccgc ttctaattct gcccgccgcg ctcgccctcg cgggctgcgc
     6601 ctccatgggc ggaaacgtca aagggaactt catctgccgc gcgcccgatg gcatttgctc
     6661 gccgacctcg aacatcgacg atcaggcgat tgcctccatg atgggtggcg ccgcagcggg
     6721 cgccgccgcc ccgattgcag cgaacgctcc agctgcccat tccgcagcat cgcttaaggt
     6781 cgttctgcct gcgcggacgg atcgtttcgg gcgctggcgt gatgaaacgg tggtctacgt
     6841 cgagccgcag cttgctgcag gtcactccgc cgatcgtcag ctggctggcc ccggcgcggc
     6901 gatccctgcc cggttgtcgc tggttgagct cgcggcgggc gcgccggcgc cggcggagct
     6961 ggccagtgtt tcgcagccga catccgcaaa gcctataacc ggtgaggttt tccgggccga
     7021 agtcgaacgg cggctttctt caaccagaaa gggtcccgag tccgtcgacg ttccgccatc
     7081 cgatgcggag acggcggcgg cgattgcagc tactgcgcag ggggatacgg tcgtcaccgc
     7141 accgggcctc ggcggacaac ccgcaccgga cggcaactga tggcgggctg gctcgataag
     7201 ttcctcggcg atctgctggg cggccctcgc aaggtcgaca aggcgttgcc gtcgaccgca
     7261 attcccatgt tcgccgattg gcttccgtat cggagtttcg atccgaagac ggggctttat
     7321 tataatagcg cctcgcgcgg cttcattctc gaagtctcgc cgttggtcgg cgccgatgac
     7381 cagaccagcg aaatcatcgc ccaattcctg tcggaaggca ttcctgacaa gacctcgatc
     7441 cagatccttc aatggatgag cccccggatc gccgatcgca tggtgcagtg gttcgtgccg
     7501 cgccagatgg caaagggcgt gtacgaacgc attgcacagc atcgcgcgga ctatctcgag
     7561 cagggcgttt ggtcgagcct ctcgagcgac gcgcccttcc accttcggtc gcatcgcgtc
     7621 ttcatctctc tcggggttcc ggaaggcgcg aaggtcagcg acgaagagct gatcgcgctg
     7681 cgcgaatcca tgtcgtcgat gttgcggtcg atcgatgtgg agtctcaaat cctcgatccg
     7741 gtcgggctct tgaagctcat cgatgacttg acctcaccga ccacagtttc gggcgaggac
     7801 gtggtggatt acaaccgctt cgatccgatt gccgatcagg ccgtgcgccg cgatatggag
     7861 atcgtcaccg atccgaaccg gattctggtt cgcaccgaac ggtttcgcgc cctcggcgcg
     7921 ctacaagacg gcacgccgga aatcggcgag gtcgttcccg accgtttcga cttccgctgt
     7981 tatgcgatcc gaaatctccc gcctcgttgg gcgccgtggg acacggttcg cctgattggc
     8041 gatccctata tcgacaagct ccggatgccg tgtccgatcg ctaccctgct cacggtctac
     8101 tatcccgacg agcaggcagc cactgccaag gcgggcttca agttcatgcg cacgacgagc
     8161 ctcgcgaatt ctcagagcgc caaattcact ccgcaggtcc gcgatcagtc gcgcgagtgg
     8221 gaatatgtcc aggaccagct tcggcagggg cagaagctgg tgcgcgtttt ctacggggtg
     8281 atgagcgttt cgccatatgg cgagggcgac cgcaacgaac gcaatctcaa ggcggtctac
     8341 aaggcagcgg gttgggagct ccaggacgag cgctatttgc agatggctgg cctgctggcc
     8401 gtcatgccgc tgacgatggc caatggtttg gctagggact tcgagcgcct gaagcgcttc
     8461 cggacgatgc tgacgacgac ggtcgccaac attgcgccgc tgcagggcga atataatggc
     8521 ggtccggtgc cgcacatgct gcttctcggc cgccgcggtc agcctttctt ctggtcgccg
     8581 ttcgagaaca ccgccggcaa ccacaatgtg gccgtctttg gcaagtctgg ctcaggtaag
     8641 tcggtgtttc ttcaggaaat gacgtcggcg ctggtcggcg ccggcgcgaa ggtcatcgtc
     8701 atcgatgacg gccgaagctt cgaacatagc agcaagctcc agaacggcga atttgtcgag
     8761 tttacgatga gctcgggttt ctgcctcaat cccttctcga tgatcgacga ggaagaggca
     8821 aaacgggacg aggattatcg gctcgactgc attgcgatgc tcaaggcgat catcggtcag
     8881 atgggcaggc acatcgatcg tctcgacgac acagagcgtg gtctcatcga cgccgcggtt
     8941 aatgcagagt gggaagcgaa cggcattcgc ggttcgatcg atggggtcat ttcgtcgctg
     9001 aaggacaccg gacatgtgca gggcgaggcg ctcgcgatcg cgatgcgtcc ctttgcagat
     9061 ggcggcactt acggccgctt tttcaaaggt cagtccactt tgaagatcgg ggcgtccctc
     9121 acagtgttcg aactgtcgga tctctccgct cgcgaagagc ttaggtccgt ggtgctggcg
     9181 gcaatcatgt tcctgtcggc ccaggcgatg cgcaaggatc gcaacacgcg caaagcgttg
     9241 ctgctcgatg aagcttggca gctcctcaag ggcggctcga tggccgattt cgtcgaaacc
     9301 tatgcgcgaa cctgccgcaa atatggttcc tcgctcatca ccgcaactca gtcgctcaac
     9361 gactattaca aatctgaagg gtcgatcgcg gcgctggaaa acagcgactg gtttgtgatc
     9421 ctgcagcaaa agccggaaac gatcgcagat ttcaaaaaac tcaaccggtt cgacatgagc
     9481 gatgcggtcg aggcaatgat gcgatcgctg aagcgcaacg gcaccgagtt ttcggacgtg
     9541 atgatcaaag ggccggagat gctcgcgatc ggtcggctcg tgctcgatcc gtattcatcg
     9601 acgctttatt cgtcgagccc acaggtcttc gcttcgatcg accgcaaggt tgcagccggc
     9661 cttaccatgg cggaggcgat cgaggaggta gcattccccg gtcgagagaa tgcccgcggc
     9721 ggccgcactg agggatatgc ggaggcagcg gaatgaccgt ctcggccgca ccgctgccgg
     9781 accgtgcgcg cgctgtggat attcggcctc gcaagctgct ccaggatctc gcctattcgc
     9841 tggtctcggt gacgtgttgg ctggctatca attgccttgc tgctgctggc ctcatcctag
     9901 gcctgtttgt ccttatggcg aacgccagcg tcgaccagtt cttcgtcgag gcggcaaatc
     9961 tttcgaacca ttatgttgcg gccgacgaat tgcgtcgttc cgaattcgct gaggtccttc
    10021 tgtacctctt cggcggctgt gtgctgtttt tctgcatcac ccggcgcggc gcgctgatgg
    10081 ccgataaggc atccattcag ggggaaattt ccaatgactg aacaactggt tttgaaggtc
    10141 ggcgacgagc cgctcgttcc ggcaaccgat gctactgcac cggcggcgcg gcggggcggc
    10201 ttcgccggtt tcacctgggg ccagcttgca acggggaccg cattgatcgc cgctcttgtc
    10261 tggggcgggt ggacaacgcg cgagctgctg attctgaaag agcgccgtat cgtttcgatc
    10321 agtctggctg gcatggccaa tgacttcatc atggccgaag cgcggtcagg cgcgagcgcc
    10381 gaacaagtcg atgctgatac gcgccatttc atgtcggcga tgcagaagac tctgcaggat
    10441 cgggccgccg ccggtgagac gatcgtcgtc agcgaagcgg tcgtggcggc gtcggttccc
    10501 gacattaccc ctgaggtccg gtcggccgtc ggcgagttca tcaagcgtaa tccaccacct
    10561 cgcgtcggcg gcatgacggg cgctccggcg atgccgggtc ttgcccccac tttgccgccg
    10621 gcagcgcagc cgatgcaggc gccagcgccc gcaccggtct ttggcggtgg cgaaggtttg
    10681 cttcccggcg gaggccagtg acggaatggc atgggtgcgc tctccgttct tgcggaattc
    10741 ggtgattgcg atctgcgccg tagcggggac cgcggcttgg ttgtcgctcg acaattttgc
    10801 gcaaacccac ggcttcctca tcaaccggac gccatcattg cccaattggg cctatttctt
    10861 cgagcgcgga aagcgcgtgg agcgcggcga aattgcgttc ttcatgccgc cgtcgaattc
    10921 gctcgtccgt gcgcatttcg gggccgagcc gccagcgttt ggcaagatcg cctatggaat
    10981 gccgggtgac aaaatctcgc atcgcgggtc ggacgttatc ctgagctggg aggctggccc
    11041 gcacggcgat gccccgtcgg aacgcgttgt agggaagctc aagcctctgt cgtcgagagg
    11101 ggagaagctc gaagccggac cgaccggcgt gattcccgac ggctgctatt acatgggcag
    11161 cccgcatccc gatggctttg atagccgcta tgccgcgatc gggttcgttt gccggcggca
    11221 aattcttggc gtctcgaaat ggtcactcct gtgaggcggc gcctggttct tcttggagcg
    11281 gtcgtgtgca gcgccttcgc accgctggct gcggcaaagg actatggcca gcaaggcgcg
    11341 gtctttcgta tcaccgagcc cgatcttctc cgcacaatcg agcagaagct gaagacgctg
    11401 gaagccagcg gtgggattga gcggatgaac gccgagctca cgcggcgcac ggaagccggt
    11461 gtccggcgtc ctcgacccgt tgcgggcatc gcactggcgg tccgcgcacg cagttggatc
    11521 ttcgatccgg caatggttgt tgagcaggat atttacgaca acaagggcaa tgctatcgcg
    11581 cgcgcgggcc agcgggtcaa cccgatggat ttcatagcga tccgccaaaa gctggtcttc
    11641 atcgacgggg atgacaggga tcaggtcaac tgggccctgc ggcgcttcga cgatcagtcg
    11701 gcaaagctca tcatgatcaa gggcgccccg ctcgacgcga tgacgcaaca tcagcgccgc
    11761 ttctatttcg atcagggcgg ctttcttgtc ggtcgcttcg gcatccgcgc cgtgcccgcg
    11821 gtcgtcgagc cggaaggcaa agccatgcgc gtttcagagg ttccgcttgg ggttggccga
    11881 aaatgaggat tctcagcatc cgcgctggtc ggcgtgccat gtccttcatg ttgcttcttt
    11941 gcgcgctcat ggctgtctcg gcctcggcgc cgccggcgcg cgcgcagatc agcagcggca
    12001 ttgaggggac gcccgggaaa tgcacgggca agttcgtgaa tccggtcacc gatgtttgct
    12061 ggtcatgcct cttcccgctg tccgtgggtg gcctcaagat ctttccgtcc gatcgcgctg
    12121 ataccgacaa cccggatttg ccaatctgcg cgtgcggatc gccgttgccc aggatcggtg
    12181 ttgccatggg cttttgggag ccggttcgcc ttgccgacgt gaccatgaaa ccgtggtgct
    12241 tcgccagcct cggcggcaag aaaatctcgc cgggattcaa tatcggtcac ggccgcgccg
    12301 ccgatagcgt ggacgggcac gatcgcggcg cgaagtggca cgtccattgg tatgtctatc
    12361 cgctgcttta ctggatggag atcctgacgg acgttgcttg cctcgagcaa tcgtcgttcg
    12421 atatcgccta tgtcacggag atcgatccgc tttggcagga tgatgcgctg accgcgctca
    12481 tcaatcccga agttgccttg ttcgccaatc cgttggcgca aaccgcctgt tcggccgatt
    12541 gcggtgcggc gactctgaag ctcccgctcg acccgctctt ctggtgcgcc ggctgcctcg
    12601 gatcgatgta cccgatgaac gggaacatct ccgagcatat cggccatgtt cagtccagcc
    12661 gcctcgccct gtcgcgattt gctttcaagt tgcatcgcca gggcatcgcg tggggaacca
    12721 tggggaagaa ggggctgtgc ggcaaatata tcatgccgat catgcgcaag cagcagtacc
    12781 ggtttcaggc cactgtgccc tcaccgatgg tcaagggccg ttgggcctgc ccgccgatcg
    12841 gcgcctccga catgaagccg ggatccggca acacctatcc cgctgtcggt gaagacatgg
    12901 gttatcttgt gtggaggaag cgaaattgct gcgtcctttg aacaattggt ctcgcaaggc
    12961 aaaggtcttg gcggccgctg ccgccttcag cgttattggc ggcgtcgccg tactggcaca
    13021 aaccgtggat gggctggatc ttcaagcggt gctgagccgc ggcgaagccg ccgacccgga
    13081 tgctgaagcg ctcgtccgcg aggtcgcgcg gcgcggcgag gagatgcgcg aagaggcgaa
    13141 gtccgccgcc gatggcggga atagcaatct tcgcgacaat aaagggatcc tcggtggcgg
    13201 acctgcgggc ccgatcgact tcgatcagat gctgcaggct tcgaacgatc tcgaggcgag
    13261 cgataagggc gccccccagc tcatcgtgtt cgcgtccttg tcgatgccgg aacagagtct
    13321 gaagaagctc attcgtgaca cggcgaaagc cggcgggacg gtcgtcttca acggcttccc
    13381 gggcaactcc atgaaagcct tccagcaggg cattatgaag gtcgtcgaca atgaagatgc
    13441 ctacggtagc attgggatcg acccccggct atttcgcgct ttcgacgtga ccgcggtgcc
    13501 ggcgatcgtc gtcgtgacgt ccgagttcga gctttgtgat gggttcaact gccggacctc
    13561 tgtgccgccc ttcgatcgca tgagcggaaa tgtgccgctt gaatacgcgc tggagacatt
    13621 tgtcgacggg cggggcccgg gcgcgccgat cgctggtcag gcgcttgcac ggctgaaggg
    13681 aggccggggc atatgagcga caacctgctt ttgggcgccg tgcagcgctg gacccgatgt
    13741 gctgtcggca ttggtgcggc aatatcgctg tcgattgcgg cgcccctgac tgcactggcc
    13801 cagacggcac cggcctcggg agaaatcacc gccgcgggca aggacggcga agcctttgcc
    13861 aaatcgggga aagaaggcgc ggcctcgatc cccgaccagg aagtcaattc cgacgtcatc
    13921 cctggctacg cgactgcacc tgatgatcta tcgaatttgt tcggtagcga tgacggagcg
    13981 ctgaattcag cgggcggcag cgccgtcggg aacgaagctt ggcaggtgat gatgggcggc
    14041 gacgccaacc gttcgcaggt cgatccagcg tggctcgagg acattcgcca gaagggtcag
    14101 gagatcaacg acaacgccgg aagctcggag ctcggtgaaa atatctccaa cactcccgga
    14161 acctgcgaag aggtcaaagt cggcgaaaag caggactatt atgacgcgac ttgtgacgtc
    14221 ggcgttaccg taaccacccc ggatcgcgtc gaagaactga gctgcccgga tgggtatacg
    14281 ctcgtcgggc ggacctgcac taagacgctg acgcagccgg cggacgtgac ctacacttgt
    14341 cctgatggtg gcgttctgca gggaacgact tgcgtcgtta cccagcccgc ggccgtgtcg
    14401 agctacagct gtcccccggg cttcagcctg cagggcacga cctgcactcg cacgacgacc
    14461 gagccggcgg tcatcaccgg atatagctgc ccggcagact atatgctcga aggtgatcgc
    14521 tgcgttcgca ccatccagca gtcggccacc ccgaactaca actgcccggc aggatatgtt
    14581 ctcgagggca ccacgtgccg gtcgaaatct acatatgcgg cgactgccag ctatagctgc
    14641 ccgaacgggt ataatctgac aggcacgacg tgttcacgga cgctctcaca gccggcggaa
    14701 gttcgctata cgtgccctgc aggctacgac ctctccggca cgacctgcac gcaaacgggg
    14761 acgtacactg ccgttcccaa ttacagctgc ccgtcgggct atacactcgt cgggacaagt
    14821 tgcacggcga caggcagcta tggcgccacg ccgaattata gctgcccgaa tggagggacg
    14881 ctgcagggga ccgaatgcgt cacttcgggt agctacgcgg cgaccgccag ttacagctgt
    14941 cccaacggcg ggacgctcaa cggcacgaac tgcatgacga catcgcagac gccgggtacg
    15001 ccagtctacc agtgcagcgg ctgggccatc ccgatggacc cctatacctt cgagggccag
    15061 acctattgcg gcatggctaa aaccggcaag acctgcccta cgggctggat cggcttctac
    15121 aaggaaggtg ttgcccaagg ctacacgggc cggaagtgct tcttccatcc gaaggtcaat
    15181 tatacatgcc cgggcggtac tgtccttgaa ggctccatgt gcacgatggt ctcggcaacg
    15241 cccgggaccg tctcctatag ctgcccgaac ggcggcacgc tcaacgggac cacttgtcaa
    15301 acgagctctt cgagccccgc ctcggtaaca tacagctgcc cgagcggcgg cacgctggct
    15361 ggcaatgttt gtaacaccag cagcacgttg cccgcgactg tcacgcatac ctgccccagc
    15421 gggggtgtgc tgaatggcac gacctgcacg atcgtcacgt cgacgtcggc gacaccggtc
    15481 tattactgcc ccaccggcta caacctctcg ggaacgactt gctcgcgcac cgatgagacc
    15541 agcgccaccg tcacctatag ctgcccgaac ggtggcagtc tggcgggctc tgtgtgcacg
    15601 atcgacctcg cgcagcctgc cgacgttaca tatagttgcc cggcaggctg ggaagtccag
    15661 ggtgacaagt gcaagtcggt gctctcgcag ccggcgacac cgatcttctc ctgcccgacg
    15721 ggttacagcc tagacggtaa tatgtgcagc aagaccgaga cgcagcccgc gaccccgaat
    15781 tatagctgcc ctaccgaatt cacattgtcg ggtacgacct gcaccaagac ctatccggca
    15841 cagcccaatt atagctgccc cgccaattac accctctcgg gcacgacgtg ttcgttgact
    15901 ctgtcgcagc cagcgacggt caccatggtt tgcccgcaag ggaccaccga gcagaacggc
    15961 acctgctacg gcgaaagtgc tggccagagc gagtgcgccg agctcgaggc caatcccaaa
    16021 tgcagccact tgcgcgacac ctgtctggac gaagagccca acggtccgtg caaggtcatg
    16081 gaacgcacct tcaagtgccc gattccgaat tcgccgccga ccgacgtgaa ggaatatgtt
    16141 tgtggcggtt cgatgtattg catcaacgga agctgcagcg agatcgagga tgaggcgtcc
    16201 aacgagttca aggatgcgct cgtagcgctc ggcgcgatcg atcaggtcgg caaggaattc
    16261 gatcctgata cgctcggcct gttcaaaggg acgcgcgaga cgtgccacaa accggtgttt
    16321 ggcttggtca attgctgcgc cggcaaggtg tcaggtcttt tcagcggcgg tgttgcggcc
    16381 gcggcggctt gggccgggtt gagcggcggt ccggctgcgc tcgcgggcat cgcgacgcaa
    16441 ttcctgacca ctttcctctg ttcgaacgaa gaaaagcagc tcgatgtgaa agaccgcctc
    16501 gggctgtgcg tttcgatcgg gagctactgt tcctcgagct tcctcggggt ctgccagacg
    16561 aagcgcaagg cctattgctg ctttgaatcg aagctcacgc gcatcctcca ggaacagggc
    16621 cgcccgcaaa tcgacaagcc gtggggcaag cccaaggaag agcaatgcgc gggcttcacg
    16681 atcgatgaat tttcgcgcct cgacatgagc aaaatggatt tctcggacat ttatgccgac
    16741 ttcctggaag ccgtgaagct cccggaagag gccgagatgg ccagcgaaat tcaggcaaag
    16801 atcgatgctt actacaggca gaatggaccg ggagggaatt gacaggatga ccgactccac
    16861 gccgatcagg gccgcgatcg acccgacgcc ggcaaataat ccaccgcggt cgctgcgcgc
    16921 ggccgtggcg gagcgttcgg attttccgga cgtgatgatt cacgaatgga ccgttctgaa
    16981 gctgtgcagc ggcgatgctc cgctcgaggc ggatgagatc aagtatttgg ttgatgagtt
    17041 tttttctcct ccattgacca tgctcgacct tgccgagctg gttgacggcc tggtcgggaa
    17101 aagttggctg gcgtgggacg tcgacaactt gaagctccac gcaactgcga cgggccgtga
    17161 aattttggcg cgcatcggcg gctcgttcag gctagccgac ttctctgctg attgggtcag
    17221 catggctaaa aggaggccgg acagatgacc gtctgcctgg ccagcaaggg agatgccacg
    17281 catggagaag gaagaattcg accgcttgag ccccgaggag cagaaagctc atctcgcgcg
    17341 ggtcgagcgg tacataatgg cccctgggca ctggcccgag gtgagcagtt tacagctcgg
    17401 cgttctgatg cgcgccgggg gagattgccc ggttgccgtc gaagaggtca tgctctcgat
    17461 gcaggactgg tacggcatca acccggaaga gctgtcccac acgatcaagc cattgatcga
    17521 gaaggaatgg atcgcgttca acgaggaggg gtttgtgcac accactccgc tcgggcgcgc
    17581 ggttgtagaa atgcacaaat cgccgctcac gaaagcggtc atgtggtcgc tgaaaagcgg
    17641 catttccaac atggaggagc aagatgaaga aggcgatccg tagttcgctc gcgcttgtat
    17701 gcgccggctt ggtcgcagcc ggctcaccgc tttatgcgca gtcgcaacca gagtcggccg
    17761 aatcggcatc atctcccaat gaccgatcgg ccgacgacag cgaggcagca tccggtgatt
    17821 tctattgcgc cgatcgcaaa ctcgggacct ggttctattg cgaaaagccg aaaccacgac
    17881 cttccgaaaa gaagacgccg gcgccgagcg caacagcagc tagcgaacgt ctagcctcga
    17941 tctccaagga attggacgag ctgaaggcac gggcgatcct tgagccctcc gaagaaaacg
    18001 taactgccta tatccgcttc cagcgtgagc agttggatcg cgcgtcgatg ttctcggacg
    18061 tctggcagcg gtccatgtgg caaaatcccg atcttgatta tacgcttcag cgtccggtga
    18121 cgaccctcgc gaagcgtgcg tggatcgaca accgcaaggc cgagcaggag ctggcgctcc
    18181 gcaacctctc gaagcgctac gggatattct atttctacgc gcagagttgc ggggcttgcg
    18241 acatattcgc gccgatcctg aggtcgctcg ccgacggcag tggcttcaac gtcgtcgctg
    18301 tgtcgatgga cggtggcccc aaccgcgttt tccccaatta cgtcgtcgac gtcgggcagc
    18361 acgccaagat gggtctgagc tccaaggcca cgcccgcgct cgttctttac gacaccgttc
    18421 gcaagcgccc gatcccgatc ggcacgggga tcttgtcggc cgacgaaatc accgagcgca
    18481 ttttcacgct cacaagcacc aaagcgggga gtgacttctg atggctgagc ttacgttcgt
    18541 cgcgcggatc cgcgtcgcgg tgtcccgcat tggaatcggc gtaatcgcgc tctctgtcgc
    18601 agctgcgccg gcgcaagccg gcgtcggtga tgagatgagc gaattcttca acgatatggg
    18661 cgcctcggcg aatgccaccg gcgcgactgc ctatcaggga cagtcagccg gctactattc
    18721 gctggggtcc gtttgggcgc gtttcccgca aaagagtgtg tatccagcca atctccagct
    18781 cccgaaggtt cgggcgggtt gcggcggcat cgacatcttt accggctcat tttcgttcat
    18841 caacatgtcg gagttcgtcg ccaacctgaa agcgatcgcc aacaacgcga tcggcttcgc
    18901 cttcaagctc gcgatcgaca gcatcagccc gcaaattggc ggtgtcatcg atcagatgca
    18961 ggacatcgcc aacaaggtga accagttcaa catgcagagc tgcgagtccg ccgcggcggc
    19021 ggtcggcgct gtgtggccaa aggtcgacgc ggccgaagat cgtatctgcc agtcgatcgg
    19081 tacgtctgca gggcgcttct ccgactgggc gaagtcacgg cgcggttgcg gcaatggcgg
    19141 cgaacggtcc tcgacgatca attcgaacac ggacccggcg ctggcggcga tgacacccgg
    19201 gccgaaaaac tatgcctggg acatgatcaa gcaatcgccc ctcgaaagtg ccgatcgtca
    19261 gatgcgtgag cttgtgatga cgctgacggg cacgatcgtc gtcggcaagc ggccgtccga
    19321 caatgatccg ctccccatca tttatcgcgg cgagggcgat cctgcgatcc tcgatgcgct
    19381 gctcgacggc gacaagccga tcgacgtctt gggatgcgac gaaactgata agtgcctcgg
    19441 ccccggcaga cgcacgatcc cggcgctggg cggcacggcg ctgcgtcccc gtgtcgcggc
    19501 cctgatccag tcgatgagtg acaaggtgcg gacgaacggc gcgctgacca ccgaagagaa
    19561 gaacctcctc ggcgtcgcca gcatcccgct atacaaggtg gttgccgtgc aggccgcctc
    19621 cggcttcaac ctgtcgccgg gcgagctcaa ttcgctcgcg gaagtgacgg cgatcgatct
    19681 tctcaattcg attgtccagc ggatgctcga ccaggttgcg gcggggcagt cgaataatag
    19741 caatcaggcc gatgcggaga acctgcgtca gttcaatgag cagctcgcgc gcgtgcgcca
    19801 gcggctgttc gagcgcgaaa gcacagtcaa tcagcgggtc acccgaacct tcgagatcgt
    19861 cgatcgcgcg atgatgatcg aaagcacgct gcagtctcgg atggctccgg ggatggccgc
    19921 atctctcaat ttctcccgcg ctctctcggc gcaaggtttg cggccctgat ttcgggctga
    19981 aggagcgcgt gcgatgatcg aggtcttcac ggtcggcggc ggggaatatc tcgtcaacgt
    20041 cttcaacgcg gttgcgtcgt gggctggctc tggtggttac cggggcctca ttcgcgtcgt
    20101 gatggtgatg gcattcactt gggcgctcct tgtcacagct tggaacatgg atccgcgagc
    20161 cttgctcaag tggttcatgc aggcgacgtt gatgtatatg gtggtcatgg tgccgaccat
    20221 ttcggttaag gtcaccgatc gcaccaatcc cggcgtgact gctgccgtgg tcgataatgt
    20281 tcccgtcggc ctcggcctga tcgcgggttt cacgagccag atcggagatt accttacgcg
    20341 cgcggccgag accgttttcg cgatgcctca ggtcctgaac tattcgacag ggggcatgat
    20401 ttatggcgca aagctacttg atgcgacgca gggcctccgc atcgacgatc cggtctatgc
    20461 gaccaatctc aatgagcatt tcaagcagtg cgtcttttat gacgtccttc tcggccgcaa
    20521 gacatatgac gacattctga aatcgtccga tctgttggcc gcgatgggac cgggctcagt
    20581 cgctctgtcg cagcagtata tctaccctga cggcacgtcg gggatcataa cttgtcagac
    20641 tgcctacacg ttcatcagcg ctggctggaa cagctactat gcggttgccg ctccgaagat
    20701 tgccgcccaa tttttccccg gcattcccgc tgcacaggcg actgcccggc tgaacaatga
    20761 catgaacgga atgggcgcgg cagggatggc ctcgagcagc ggtcagcttg tcaggcaggc
    20821 gatgttcatc aatgcgttgt cggaggcgcg cgacggcttt gcgagcggtt ccgcacaagg
    20881 cgcgatcgac gcttttgctc agacgcgcgc ggacatccag acccgcaaca cctattcgac
    20941 gatcgccggc ggtgcgatga agtgggttcc gttgctgaac attgtgctca cggttgtctt
    21001 ctattcgctg ttcccgatcc tgttcctgct gatgttgctg ccgaccaacg gcatgacggt
    21061 cgccaagggc tatatcaccg gcttcttcta tctcgctgcg tggggtccgc tgttcgtcat
    21121 cctgaacatg atcttcatgt cgcgttggca ggattccctg gcgtcgtggg gcgcgagtgg
    21181 cggcctcact gccgccaatt tctccggtat ctcggccttg aaccaggacg tcggcgcgct
    21241 cgccggctac atgataatgt cggttccgtt tatcgctgct ggcatggcca agggtgcgat
    21301 ggcgatcgcg tcgcattcgg cgagctttct ttcgccaagc cagaatgctg cggagcaggc
    21361 ggccgccgaa gccaccaccg gcaattatgc ttatggcaac gcctcgctga tgaatcggca
    21421 gatcaacacg ctgaaccgcg accaatattc tacggcgccg agtttcaaca ccggcgccgg
    21481 cacgatccag cagcgatcga gcgacggcgc gttcacgcgc tacaatgaag acggcacgtt
    21541 cgcctatgac acgagccagg gtatttcgaa cctcgggttc ggtatctcgg ccagccaaga
    21601 tttcgggtct cagctgcaaa agggtctcag ccaagggtcg agtgtggtcg aacaaaagcg
    21661 ggtcgccgcg aacgatagct gggccgcgac ccgcacggaa tcggcgcgat tgtttgacac
    21721 cgcgcagacg tcggccagcg ccaccaccga agaggggcgc tctctgcagt cgtcgatcgc
    21781 gcaagtccag gatctgtcat caagctggtc gaataccctt caaacccaat tcggtatgac
    21841 gaaaactgag gccgatcagc ttgctcgaga cactgtattg acgggcagcg ctggcattgg
    21901 tgcgtccggt gccctgagtg ccgggaaagg cggaccgctg tcggggtctg cggatctcaa
    21961 agccggtctt gatggacggt ccagcgatac tcgccgaacc gaccaagggg tttcgacttc
    22021 gacacaaaat accgacgcac atcagtggct cgaaaaggaa tccaattctg aagcggcacg
    22081 ccaagcgcgc gagagcttct atcgcgcaac gacgtcctcg acagatagcc aagtccgcgg
    22141 gcttggtcag gaagtgtccc aggaccttcg tcattcgcaa agtctcagca atgaagcgtc
    22201 gcgggccgaa gaccagtatc agcgctggtc gaacgaattc tcgcagttcg agcgggacgg
    22261 ctacgcgctc aacaagaacc ttagccaaga cttcgtgcgc tatgctcaaa cagctatgat
    22321 ggatccccag aaccgtcatc tcgatcagag ctatcatccc ggtctcatca atatgacgcc
    22381 gacccaggct attacccagg atgctttggt gaagcagttc atggacgatc gacttgagca
    22441 aatgcatcgc gatctcggtg tggtccccga tgcgcctgcg gcgacgatcg caggaccgtc
    22501 gttctcaact gccgacgaag ttcgagcgat cggcgctgcc ggtatgcagg aaatcgctgc
    22561 tcgcgggcca tcggttgatg ttgccggctc cgcgcgtgat gttgggcttg aagacaacgt
    22621 aagcggccga ctgggcggca gcgtcgcgag gctcgacgaa tatgggcatg gaatgagggc
    22681 aactttgctg ggcgaccggc gccaggccga taatctcggt gcgacagcag aagcccgcaa
    22741 cgattcatgg gtcattagaa ccatgccggg cgttgctggc gctctgcgcg ccctcggcat
    22801 agacggagct ggcgaggccc cgcttggagg acagcgagtc aatctcggtg gcgttgcgtc
    22861 cgccgctggc ctcgacatca agccgggcgc caacctcagc tccatggact caagcatggc
    22921 gccggcgttg actgcggccg ccgcgtcagc tcacgctctc ggtctgccac agcgcgtggt
    22981 aacatctgcc cgcgatagtc agcatacccg cggcagcttg catccagatg ggaagggtgt
    23041 cgatctgcga ggaaacgaca tcagcgttgc gcaaggaaag caatgggccc gggatgtgcg
    23101 cgaacggcta ggtccagatt acgacgtcaa tttcgagacg ttcaaggaca atccggccaa
    23161 taaccacctc catatggagc atgatccaaa accccggggt gggcgcggtc gcaaacgata
    23221 gtgctgagtt gccggtaggt gaactaccgg caaccgtcag tggcttccca ccgccaagat
    23281 aagggcgaag tacgaggcaa tcagcgcgaa agcggcatag agcgcgcgcc gcccccaagg
    23341 cccaagcgcc gtcttgccac cgctatgcga catgcgcgca agcccgtcct ctgcagccga
    23401 cgcaatgtaa agcgggtgcg atgagtcgta gtaccggcgg ctccagtccg agccaaatgg
    23461 cttaagctga tcatccatga caaaatcctt cacaagcgaa catacaggaa acaccccgaa
    23521 acggcaagtt cctgtatgtt cgtggggaag gtgcgctgga tgagtctagc cgctgaggca
    23581 cggagccgct tgatagtcga cgtcgtcatc atgacgtcgg cacgagatgc gcgaggtcgc
    23641 aatcgctgtt gccgcaaatg aggagcaagg tgggctttcc ccaagcctgc gcagcacaga
    23701 ccggacaacg aaatttgatt cgattggatc ggttgccggc gacgggttct aagatgccgg
    23761 taagtccagc tccgagcgcc gcggccgatg atgtcggccg cgaagcggcc gcatcttcag
    23821 gtggaaagcg atcgcaccag gaaatcttga agttgctcgt taggagatcc tcagttgcgg
    23881 caatgaacgc ccctcccggg atgggatagt ccgccatctt ctgccctgtc cgtgcaccgc
    23941 caggctcgcc agtgctcgac ggcatgagcc cgatcgcttc cattttgtcg gcccattctt
    24001 gattgtgata tcccggcctc gaaggattgc cgaaatgggc ctgccatgcg tgaaccatct
    24061 cgtgcacgat ggtctgaagg atctcgagca gaggcacgac ggcgaagtag gacggattga
    24121 ttgcgatctc atcggtcgat cgctggtcga ccgtgtgaac gaatctccgg cgcgagaaat
    24181 agccatatgt ccgcttttcc cgctgcatgg tgatcagaca gtggggcaat ttgccgtcga
    24241 agagcatgcg attgtagtgc tcgaatgcgg cctgcagctc ggcatacgcc tgcgttgtcg
    24301 gatctaacgg cgcgttcgga ccgtttcgca tgggaggaga attgttttgc acaaaacaat
    24361 gatagcggcg cgggcccaat cgtcaatgtc gcgagccgca actgtggcag acagcagcgc
    24421 gatatcagcc ccctgaaagg tagtcatttt gacaaacctt ggcccaaatt gtccgaaatt
    24481 ttggattcac acacagcaaa aaaatcccgc ctcggcgata tgccgggcgg gaagacgtgg
    24541 tgcttttgac tttttggatt gcgaggctgg ccgctataga aagccccggt cccgactatt
    24601 tcgcagctcg atatccgaca tgcgcgtcga tccccgcctg atccaaacac gggcttttcc
    24661 tccgctcccc ggctttatgt cgacgtcgcg cggatcaatg ttgttgtccc gcgcccagtc
    24721 atcggcatcc ttctgcgagc cgaactcgcc aacttcgtca aagtcgaaac cgctcattgc
    24781 cctgactcct tattgattgg gtcgttccga tcgtcggtat tgggccctgt cggtgccggc
    24841 gccgtgtcga cggtcttctt gtcggcgccc gatggggcga gctggatgcc gtcttcccaa
    24901 ggggtcccgg tgaactcagg ctgcccttca acataggtga cagttgcgcc tgccgtcgac
    24961 cggacatagc ggacgacgtc gaggattgtt ttgttcgtcc aattgtcgct gttcccgacg
    25021 atcgcctgcc caagcgccct gcccagtcga gcatccaggc cgccgccctt ctccttgatg
    25081 tcgatcgcgt agacgtccag ctgatacccc tcaacatagg ggaacaggca catcgtaatc
    25141 atcagctgct gagagcctcg aaccttcctg acggccgagc tgatccagac ggctccatcg
    25201 cagcgtgcct gcttgatagt cgatagctgg gaagtcgaaa caaaccccgc ataagggctg
    25261 ttctcgaatg gattgaccag cgtgaagcgc ccgggctttt cgggcgcggg acccatgacg
    25321 atcggcgtca cggtgttcga tttcgagatg ttcgggttta tgccgtcctg aaggattcgg
    25381 atcatctccg cgcgcttcat cggggttttg aaatcgaaga tgtggtaaag ttctgtcgtc
    25441 cggctgctgt cttgcgccag cgctgcgatc ggcgtcgtgg ccaatgcgcc agcgatgccg
    25501 gcagcgctca gggctttcat catcgtcctc atattactct cctcaatcgt tcgggtctca
    25561 aaggtggttg gcttcgcgca cggtgctcga gcagctgcgc cggatggctt gccgatcgtc
    25621 ccagcgggcg acatagcttg ctcccgcctg cagcatcgca cagctgaggt tggtgccggc
    25681 ggcagaatga atgattgcga ccatgcggcc atatcgatcc cgtttgacgg gcatgatggt
    25741 gatggcagtc tgcattcggc gctgaagcgc ggcgcgctgc gtctcaggat cgcccggcgc
    25801 gcaaacgcgc ccctgccggc agtgacccgg cctctcggcc gcatcgatgc cgagcagtcg
    25861 cacacgttca cgaccgcagc ggagcgtgtc accgtcgatc gcgctgcagg tcccggcctc
    25921 aagcaagagc ggacgggcgg ccgtcgagga agtcatcatc tcgccgccag ccaagacgag
    25981 cagtattgcg ccggcctgtc cgaatattag tgcattcatc accggtcccc tccacgccgt
    26041 atccaacgaa tttcttcccg gaccgctttc accaggccaa ggaatatgtg ccccaccaga
    26101 taaagggcga gcagatcgcc gccgagctcg agcgcatcgg ccgtcgatcg ccagcgctgc
    26161 aaggtgagga tgccggcgac gtcgcccagt ttggatgcga tgatgatcgc ggcgccaaac
    26221 gcggctgtcg atgcgccgac cacccaaaag accgattcaa cgcggctgga tcccgtgcgg
    26281 gttatgaatc gtgctgtcga taatgacatt gccgcgattt ttcggttgat tgcgatcggc
    26341 gcggccaaag cgcgcgacat gattgctcgc acggcgcgcc ctcgtcctct ttgatgcgcg
    26401 gttatcatag ctcgagccct tttgatttat cgagctgggg ttcctcgcgt ggtgttggcg
    26461 gcgccggcga cgacggagtt ttgccgctat ccttttctgg cgaggggtcg gggctagaga
    26521 ggtccgtcgc cttgctttct gttttcggct cggagccacg gacatcaagg tccgccagcc
    26581 cgctgaggtc aagctttcga tcaaggtcgc tggttccaga cggcttgtcg aggtcgacgg
    26641 gcggcgccgg ggaaaagtct ttcccttttc cggaaagtat cttttcaacg gcgagctcgc
    26701 cagtagcctc gagcgcggaa ctcttgtcgc ctttgttgtt ctcaagctga gtgacgagct
    26761 ttgctttgtc gtcggtaaac agccgaacat cattctgctg acgcgtatgg ttgactagaa
    26821 acgcgcgcgc gttggacaga tttgtttcac gcgcgcccat gacggagaaa accgtctcgt
    26881 tggtgatacc ttgggccata tgggtgttga tcgcataggc gagatccata cgcttcagca
    26941 tcttgtcccc attcggcaac tcgcggacct cgccactcgc cagtctcacc gtgatgccgt
    27001 ctgccgtcac cttttcaacg ctcgcgatcg tagcattgag catcccacga ccatcgcgtt
    27061 tatcactatc tgtccagcgg atccgttcgc cttcatgcac ctcgatccgc ttctcgttgc
    27121 tgagcttcag ccgattgagc ttgcccgtgg ggtcaatctt gatgggctcg acgcgatgct
    27181 tcttcccgcg catgtcgaca agatcgactt tgccgttgtc gaagatacgg gcaatgcgat
    27241 aatcgccttt cgcaagtcca aggccattgt cgcgactgcc gacctcgagg aattcagcct
    27301 gacgccaatt gcgggcgtat cgatattcct cacgggtgag ctggaccgct tctcggacat
    27361 taaaggccct tccttcgccc tgcagcgtcc cttccgcttt caatccgtcc tgaatgatct
    27421 ggtttgcctc cgctcggccg tcgcggctcg atgggagcaa agtcgtcttt tcgcgctcat
    27481 cggccgagag tgcgagccac gcattcgcgg cgtcgccaat acgatcgtcg gattcgacga
    27541 cgcgatcgcc aaggacggca attgcaccgc ggacgtttcc ggcgtcggcg agatcagcga
    27601 caagccgcat ctcctccgtt ttctggcgca tattgatgtt tacttcgctg acgggaagtt
    27661 ggtcctggat tgcccgggcc tgcataagcg aaaagctctt ccccgcgtcg atgggattaa
    27721 gctgcttccg gtcaccgatg atggcgagac ggcctacttc catgaggttc gcgatcgcgg
    27781 tcaacttgtc catttgctcg ttcgagatca tcgaaccttc atcgaggatg agatagacgc
    27841 ccgcgagttc agccttccgg gcggcgaacg cttccgccga cccttcgccg ttcaggaact
    27901 tctcgttccg aaatataaag gcgtgcgtgg tcatcgcttc gagaccggta tcggtcttga
    27961 gatcagcgac catcttgttc tggaatgcga gggctttcgc gtcacctttg acgtcggccg
    28021 tgagcaacag cgcatctttg ccttcagcgc tgagcatcct ggccgtggcg tcgatcgctt
    28081 ccgctttggc gatgggctgc agcatcgtcg acttaccggc gccggcacgg ccctggacga
    28141 gaacaatgcg atccggtgac gacacgatct ggatcgcggc ggcgagctgc tgatgattga
    28201 gctcgcgcgg cgctgccagc tcggccaggc gcgtggccgc gacatcggcg gtcatgaggg
    28261 gccgcgcttc gccggcgccc gtatccattg ccttcaggat ccggcgttcc tgctcgatag
    28321 cctcgcgtgt ggttatcatg tcgactgctt tatcaagacg ctgggatgtc tctggaacga
    28381 cctcaccctt ttcgaccagc tccgcgatcc gccgctcgac gcgatcgggg gtgacacctt
    28441 tctcgcccag gtcgaccgct gtctttatga tgtcggcggt tttgaagact gcttcgcgtt
    28501 cgctcagaat acgaatggcg ctggcggtgg catattgggt gcgcgcggtg gccggcgtcc
    28561 ttgtgagctt ggcgagaccg gaatcgacga gcggatcgtc agggcggcgc aaatagtcga
    28621 tcagcctgtc gcggctgtca cggatcgctg aacttacggc caatatgccg cgctcgatcg
    28681 gcgagcttcg ctcctgcgat cgggagagtg cgatcgcgta gatcttgtcg ccattataat
    28741 ggtaatcgcg cgcttcggca cgccaccgct ccgcgagctc gccgcgatcg ccggcgtcca
    28801 tcttcggatc gcgagtgcgc tgcgtgatgg ctcttcggcc ctcaggactg tgcacaccga
    28861 gttcttcagc cttagccttg atgtcgttcc cgcgtttcga gaagccgtcg agcgcctctt
    28921 tattgatacg ctcgccattg ggaccgagaa cctcgaaagc gccgtgcttg ccggtcactt
    28981 ctgtcttgta gcccagcccc tcgagctttt cgcgcaatat ggcgttcgcc atcgagctca
    29041 gtaccgcgct ggccctatac atcgcgccgt tgtgccatgc gcgccacccg tcgtcgctcg
    29101 tgacctcgcc tgtcttctga tcgacctgat cgggattacg aaaccgctcg ggcaatctgg
    29161 tcagattggc gatcacggcg tggatgtggc tctgcggatc cgtggcgcgg ctcgtatcgt
    29221 gcgggaacag cgcatagacg agatttccgg ttcggaccgc gacgtccttg ccgtcgacat
    29281 gaacccgggc ttcagccaaa ttcttctctg cccaggtcat cgtctgtttg accgcagcca
    29341 tgtgtgcggc gaggatccgt ttgtctccgg aaacaagcgc gaggagcgaa gccgacttcg
    29401 gcatcgagaa tgtgaaatca cgaccgctgt cgcgggtctc cgcgtcgccg atttgtttgc
    29461 cgtcgggcaa ttccccgctc aaaatccgct cgaaggttgc cgtgtccacc gtcccggaca
    29521 gcccgagatc ctttgcgccc tctcccgccc atgctgatgc ggcttcggat tgttcagccg
    29581 tgtaatagtt gtcggatgcg aaataggccg ccgcaccgcc cgccgaatga accttgccaa
    29641 tcgatacggt catgatgcgt cagcctgcca gaaattcttg cggccgtact gcgccaacgc
    29701 ccaacgtgcg cgacgatcac gccacaacat aggtttcaag tccgcatggg acgggcccgt
    29761 ccaggtgagc ttgaggcctt ggctggcgag ccgatcttct agctcgctgg catatatgtc
    29821 ttcagctgat cccagtttag ctgccagcgt ccagccatca atgaggcccc atcgcatccc
    29881 cggtccgaca ccccaaccaa gcaccatcat gtcgaggacg aaggcatcat cggccacgca
    29941 gaggccgtaa atgagaccac tggggcggag cgaacctcca gcaacatccc gaaccaacgt
    30001 ccagctttcg aggtgccgaa gtgcaacccg cgcggaggca atatgggcgg cccgtatcac
    30061 gcgggcgttt ttgctttcgc gaacatatga gagatcgaac ggcggcgaga acagcaacga
    30121 cattcctgca atcgttcccc cagctcgacc accctgccgc ttgatttcaa ctgtaggtgt
    30181 gaacaccgct gcaattcccg cgtcgcgatc gagggcggaa agaccgagcg cgtcagcgcc
    30241 ccctccgagc cagtggatcg ttcgcccagc tgaatgatgt tcaaggatcc gcgcttcgat
    30301 ctgctcgagc acctccatgt ggaagaagct gatcatcgcg ccacgccctt cgccatcaca
    30361 attgtttcca cccgggcacg ggatcaccaa ggtgccactt cccgcaccga tcgcattggt
    30421 agggctgaag cagtgcgatc ttgcgccgcc tatccttgtg tggggcgtgc cggaacctgc
    30481 tgtgttcgac caaaatgcct ttcgcagcat cagcggcaac cctcgtccgc caagcggcca
    30541 tgcccgggca ttgtggactg aacggaaggc gcctgaagct cacggcacgt caatccatat
    30601 ccatgccgag atcgtcgctg ccgcgcgcca tgccatcggc gccgagctcg gcgccgccgg
    30661 cgcggcgatg atctgtatcg ccgaaatcca tacgggattc acggcgcgcc aattcgccac
    30721 tcttctcttc ggtcggatcc ttcggtccgc gcagctgctc cttgttcgcc agcgcatcct
    30781 gctgaccgct gctcccggta gaagcgttgc gaggtgcgac gccccggggc ggatcgtcgt
    30841 cgatcgaacg cttcgccaac gacgtgatgc cggctggctt ctgggtttcg ccctgtgctg
    30901 agatccgcgc gtccagctgc ggccgaccaa cgtcgatcac gtcacgcgca cggaccgctc
    30961 gcccatctgg caggtcgaaa acctcgcgac gatcctgacg ccccgtctcg gcttcaacgc
    31021 cgggctccgg gcggtcggca gcgatatcct gtccgccccc ttcttcgccc tctccttctt
    31081 cctcatcctc ttcgacgggc gtgaaatccg gcggcggggc ggcttcaatg aaaccatcgg
    31141 caatggaagg aaacccgaca tagggaaacg aaaccaacgt cgacgggaac ttctcaggga
    31201 aacgaatata ggcgttgaga tttgggaggc tggtgatgtc atccgcgatc accagcgcct
    31261 cttccttggt tgtcggcgag atggtggaag cgtcgcgagt ctgatgcgcg ccgtagctat
    31321 aggactcgtc catcgtgcgt accttgcgga acccaatcag ccgtgaacac tcttccgccg
    31381 tctcgcgttc tttcacccga aggatgacgt tcgtgttcgt caggctgatg atgttctgag
    31441 ccccatcgtc gccatatgta ttgcgaagac ctgcaaaatt gtggaggcca agaaggaaag
    31501 ccccgccgaa gctgcgcgcc gtttgcaagc ctctctcaag cgcgggcagt tgatgaagcg
    31561 cggcgagctc gtcgaagata aaccaggtac gaagctgccg agtgtgcttg agtgtcatga
    31621 tcgtgttgat cgcgatattc atccacaatg tgaggagcga tcgcgacata tcgaggtgcg
    31681 cgtattgggc ggttatgaaa aggatggatg cctccccatt gtcgcgcttg atccattctt
    31741 tgatcgaaaa aggttcgcca ccttcgggta ggaaacggat ggactccgcg ttagcattga
    31801 gcaccgcacg gatcgactgc gccatcttcg ctgcgtctgg cgagaccagc ggctcggcca
    31861 ccgtacccat cagctctgcg tagatcttct tgaggctcgc tttcatgagc tggtcgcaca
    31921 gatgcttgtt cgtgccgcgt ccactcctcc tcagattatc gcacatctca acgaacaggg
    31981 ttcgcgcggc cttctcccag aagccgccat ccgaaccgtg gtctgctgga ataagagcgg
    32041 tagcggcggt gacgagttcg cttcgcgagt ggcagtcatt gaacagcgac caggactcac
    32101 agcgcgcatc gttgaggttc aaaacgaagt cccgctcggg atcgtagaag gcttccatat
    32161 agtgcccggt caggtcgaag aggacgcacc gatcgccccg ctcgatcgcc tgcgccacga
    32221 cctgtcggat gagtgtcgtc ttgcccgtac cggtcgtacc ggtgagcatg acgtggctct
    32281 gttcgaaccc gtatggaaag ggcaacgtcg caatcttgta cggcaagata acgcctgccg
    32341 ctttcctctg agcgagggga agtgcttcga cctgtgcgag cgtcaaatcc gggaatgctt
    32401 cctttgcccg ttcccgaacg cgatcgaggt tgtgccgatc gagcatcatc ttcagcacat
    32461 catagtcgac gatctcgacc ccgcgttcat ggtggtccgc gagaatggat tcaccgcgct
    32521 cacgcgccca agggatgaac caccatgcga acatgcccgc cccaatgctg gagaatatca
    32581 ggctgcccca aaatgcgttc atgagcttcg cccatgcgat ctgaacgtcg ggaataaagg
    32641 gaacgtaacc catgtacgtc tggtagacgc tcccgtcccg caacgtcaca ttggcgcgct
    32701 tcatctcgtc gaagccgacg aagttccaga acgccgcaag tgccttcgta ccgagcatct
    32761 gaaattcata atcgtcgagg atcagggtca gcttgagcca cgcgaagccg agcaacaatc
    32821 cgatccaaac cagtagcgga acgcgagctc cgcgccacca catcgagaac tgatgaccga
    32881 tgagctggga gccgcgggtg aaatttccgc tgttccgatt tgcctcgccg tggcttgacc
    32941 aggcgcgggg atgacgcggg cgattgagct tatcggacat ggtgacgatc catgcgctgg
    33001 tcgacaaccg aaaggaattc atcccgccgt tcgggagcgt gcttcttcac ccattcgtcg
    33061 gcaacgagct gcatgtattc gcagatgatt gccatgcgct gcgggttgaa ctgcgggcgg
    33121 tcgcggtcag ccaaaaagag gttcaggacg cgccgaacca actctgatgt tgtgatgcct
    33181 tcatgctgcg cgatctcagc cagagacgac caggtctccg gcgaaagcgc cacggaacga
    33241 cgatgaaatt ccaaaccacc ctccctcggt agggatgggt ggtgggttct tgcgaaaggc
    33301 gaatgctggt taccagcacg cggcagaatc gtcaagaagc gccccctgcc gcgccgaaat
    33361 tggtgcaagt tgcaccccga cggctaccgg tttgtggcgg aaaaccgcgc ctttttcggg
    33421 gtgcgaaaca cacccttata ggggtttgga ttgcaccctc tggggtgtat tttgcgcccc
    33481 catattcgcg tgtattttca agatgttgcg gagcaacggc tttgcggctt gccgcatacc
    33541 gtgtgcatct cagaatgaga tatgcccgaa gtggttttct cattctgcta ttcgagctgg
    33601 aattcgggca tagcccgtca gcttgccaag gccggggcac tttgtgaccc tgccgggcat
    33661 ccaaaaggac ggcagcatga tgcgaattgc atcgactgac attgcaagat tgtcgggcca
    33721 attggtcccg gcaatctaat cgttatcgtt cggtggaacg agaccgatca ggttccagaa
    33781 cgggaacccc acgaagatca tccgttctta cggatgcgta tcggttgcga cacctgtggg
    33841 agcaatcgcc aaaaagcgca ttgcgttttg ggagcgcgaa gcgcgaaccc aaaacgctgg
    33901 cgtagcgcgc ccaaaacgca attcattttg ccgccctggg gcgatcaaac cggaacccta
    33961 tcggctgcaa cacagcagcg acgaatgttg agcaaccttc gcattgacgt tcctataacg
    34021 ggactggatt ttcacgcgct ccatgatagc aatgccgttc gattggacac cgatagaagg
    34081 aatgaagaga tgccaagagc gaagaagagt gaaggcaatt tggaagccat cacggcgagg
    34141 cgagcagcac tcgaggcgga gttggtggaa ctggccgacg ccgaacgccg agctcgcgag
    34201 gcagagcggg atgcgggtcg cgacgtgttt ctaggagcac ttgggaaggt gaagatcggg
    34261 cgcatggacc ggagcgaagc gacggccatt gcgaaggcta tcgagaagca cggtgccgaa
    34321 aagattgtgg caaagctcgc caccatctga tgatgccatc agcgcgatcg gcgatgcgca
    34381 gctcgcgcgc ggagcaagcg agaagcgagg cgaatgcgca gccataacgc gaagtcttcg
    34441 ccgacctatt gtacctgcga tgcgctgacc accggcgtag ccaggtggca agcgagcagg
    34501 tcatgttggc gcggtccggg gtcagacaat tgggaccgcc ccgcatcggc gatgcgccgg
    34561 ttgcgagcga agcgatgcga caagcgaggc cgatggtcgc acgtctttca agaattgcgc
    34621 ttcaatcttg gggctaacag cggcgatgcg aacaggcggg catgttccgc cgtgagcgag
    34681 gcggatgcaa aacctagccg ggcgtcgtga ccgacagatt ctatctgcga tgcgctgacc
    34741 accggcatag cccggtggca agcgagcagg atcgtactgc ctcgcggatg ggtcgcgatt
    34801 taaggaaccg ctacccaatg ggcggcggtt ccgtgcggcg ctcaatgagc cgcccagcaa
    34861 agttgctcgt atctgaaatc gagttccatt tcggtctcag ccagcgtctc cgcaagatgt
    34921 tcctcttgca tcgagatcag gcggcaatcg tcgttcgtca tggggtgcca gtcgatcatg
    34981 tccgcgaaat attcggtgcg atcgatccgg cgattgagtg accgcgacat gttttcgaga
    35041 tgagcgatgc gtgcattgat tgcgagttcc atgattatcc tcctgtgaag cttgtcgatg
    35101 acaaggtctc gggggagctg cggcgccgtt ccggtcaagg acggcgaagc cgccgcgaag
    35161 cggggagcgg gggagccgat tttttcgaag gaaaaattgg ggggaccgct catccttgac
    35221 ggggacggtg acgcggctcc acgctaggtc ttcaacaaca gatgaatctt cctaccgtcc
    35281 cggcacgacg ttgctggata ccgatcggcg atgcgccggt tgcgagcgaa gcgatgcaac
    35341 aagcgaggcc gatcgcggca gaacctcaaa cgcctgacat gtgcccgtgg aatgatttgc
    35401 ggcgatgcga acaggcgggc ccagtcccgc cgcgagcgag gccgccaggc gcgcgcgaaa
    35461 tgcaaagcga aagggccaga gatctctggc cctcgcttgg gcgtttcgat gaccatcgtg
    35521 gagggtcaat gtggggcggt cgatccgatc ggaatgctct cggcgaattc gaacatcggc
    35581 tgctcgtcgg ggtcgtcgaa attgagttcg aactggtcgt cgttgtggac gaatgggcta
    35641 aacgcttcgt agatgtcggg ttccatgatg cgtctcctgt ggaggctgaa ggggcggccg
    35701 agaccgcccc cgggatgatc agaaggggac gttgtcgtcc tggtcgtcgt cgtcacgatc
    35761 tgcgggtgcg ccgccggcct tgacgagcgt ggctatccga tcgacgatga gatcgactgt
    35821 gtattgacgc tcgccgtttg cctcgtagct gttctggcgg acgcgaccgg tgatgcgaac
    35881 aaggtcgcct ttgccgatct tcgcggcctt ctcgtgcatc ttgccgaagc aggtgacgcg
    35941 gttccagtgg gtgtcgtcct tccaaccttc gccgtccctg cggggatagt tggcggcgac
    36001 gtcgaggtgg gtgacttttt ccttggggtc gactttgccg acgcggccga tgatgtgaaa
    36061 ctctgcacaa ttttgcatga tgtatctcct tgggtggtct tgttgctgac gcacttcgag
    36121 gaggcggccg ttcgccgcgg gcaagctgaa aaacggaggg tccacgtagt ggaaaccgta
    36181 ggcgcgcagc gccggttttc tgcttgaggg ccaaaggccc gtcccgcaaa aggcgggcga
    36241 gctgcctacg tgcgtcagac agcaacgaga cggcccaagg agataggtgc aacatggctt
    36301 tcgcattgga cgcgtcgcgc accagggcga cccatgatgg tcatgcgcct gtccgccgga
    36361 tccccgcatg gcccgatgta caaatgggtg atgccggaat cgatctgttg ccgctggagc
    36421 atcgagcggg ggttcgcgat cgagctatgc ggtgccgttc tactccctag ccttccccga
    36481 tgcgaggagg tggacggtgg cactgcattt cggtttgcta ttccgagcgg tgggtgggat
    36541 cgatccggag cttgatcaca ttgtcgatga cacggcgcca tagcgcttcc ggcatgatcc
    36601 cgatgaaggg gctgccgtcc actgtgcctc ggacatcggg accgacccat gcaaattcat
    36661 tgaggtcgtt ccacacaacc cgggagcgat cgtccagtcc gagatgggct gtcacccggg
    36721 gcgggatcgg aaccgtgaag ccggaatcgt aatttctcgt cgtgatgggt gccgtgatga
    36781 cacggagctg gccgcgggcc ttataagtgc tcaagacaag gcacggcctt gtctttcgac
    36841 cgtcttccaa cccgcgctcc ttttcggcgc gccagaggta gacatagctg atgacgtcgg
    36901 tgggttgcgg ttcagtccat gagctcgttg aggcgggttg cttcggctga cgccgtggct
    36961 gtccggaggc tgcgggtggc ttcatcactc aattcctcgg gcgtaacagc gatatggtcg
    37021 agacgcgcga ggcgttcata ttcggaagcg ggcagcatga ccacgcgagc ggcgccgtag
    37081 cgctcgatcg cgatcgcgtg ggtcatggcc tcatcgtaat agaagccgaa acgcttcgat
    37141 acgtcgctgg aattggcctt ttcgatcggc agttccatga tttcctcatt accgttaacg
    37201 gatcggcgaa tcgccgatct tcatcatctt tactcgaaat ccgcatgtta cgtaatatac
    37261 ggaacatcga gtttcaaccc cctgcccgcg cgcgggctgg atcgcggcat gaggg
//
LOCUS       DATMSU010000245        16049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_174979,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000245 DATMSU010000000
VERSION     DATMSU010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 16049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16049
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174979"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VN034_06435"
     CDS             complement(<1..193)
                     /locus_tag="VN034_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HWV60270.1"
                     /translation="MSWTDERIEQLRSMWEKGLTASQIADELGGVSRNAVIGKAHRLG
                     LKSRPSPVKATEKAAKPVKA"
     gene            312..1184
                     /locus_tag="VN034_06440"
     CDS             312..1184
                     /locus_tag="VN034_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV60271.1"
                     /translation="MNDQPQISNPDSANIRSGPAFAEPGVPHIHTLNVPGMQALYVKE
                     VRRFFKVQLQTIWAPAITTLLFLVIFTVALGGAGRTVIGVPFADFIAPGLIMMAMLQN
                     SFANSSFSLLVGKIQGTIVDYLMPPLAVGELIIALVGAAITRAVLVGFALWLAMLLWP
                     GVHVGTDHLWAVAVFGVLGAMMLGFLGLITSVWAEKFDHAAAVTNFVVTPLALLSGTF
                     YSVDKLAPWFQTIAHGNPVYYAIMGFRYGFIGTVDSTIANPVLTAMLVLVAVNILLGV
                     ITYRLLASGWKLKA"
     gene            complement(1232..1606)
                     /gene="hspQ"
                     /locus_tag="VN034_06445"
     CDS             complement(1232..1606)
                     /gene="hspQ"
                     /locus_tag="VN034_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HWV60272.1"
                     /translation="MTPESSSELPAAVTAPLIGRARFAPGDIVRHRMFDFRGVVFDID
                     PVFANTDEWYEAIPEEIRPAKEQPYYHLLAENGDSSYIAYVSQQNLVADGDGGPIDHP
                     QIDAMFDGLDHGRYRVRAIHRH"
     gene            1785..2408
                     /locus_tag="VN034_06450"
     CDS             1785..2408
                     /locus_tag="VN034_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HWV60273.1"
                     /translation="MADVPPNISGPASGIVQRAKDILLKPKETWPVIAAEPATTQSIY
                     IPYVLVLAAIGPLAGLIGGQLFGITILGVTYHPPLATALVSAIVSYGLSLATVFILAL
                     VIDGLAPNFGGQKDQVQALKVAAYSATAGWVGGIFGILPALAVIGLLFALYGLYLLYL
                     GLPVLMKAPQDKALGYTVVVVIVYIVLFLIVGAVVGALAAPSLVTIR"
     gene            2630..3883
                     /locus_tag="VN034_06455"
     CDS             2630..3883
                     /locus_tag="VN034_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HWV60274.1"
                     /translation="MAKLTAKTVLSAKAGRHADGGGLYLLVKPTGARSWLLRVQVDGR
                     RRDIGLGSVETSALAMRSDIGTDIPLEEKSRLTLAEARELSARLRNAAKAGRDPAAER
                     KKDRKAPPSFKDAAIATHKAQTASHSWSDKTAGAFLSSLETHAYPTLGKMRVDMVEAE
                     HVANALAPIWTAKPDMAKKVRQRIATVLDFAKAKNWRATEAPRKAVGALTGKTKKGGH
                     FEAMPYAEVPGYFRSLMNETPTKGRLGLMLAIATVARSIEVREARWQHVVTDKRDWNR
                     PAELMRKNHEAHTVTLNDAALWVLAHIAEISDPLKPDTLLLPNRKGKPISDMTISKVM
                     RDAGLSSVPHGFRSSFRDWAAEQHPEIPDPVAEAALSHSVPDAVIAAYKRTKFLEMRR
                     TLLDHWSAFILSGRTNVVQLADRRA"
     gene            3976..4980
                     /locus_tag="VN034_06460"
     CDS             3976..4980
                     /locus_tag="VN034_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60275.1"
                     /translation="MTNYAKVPVRFGGEIRRVTDLQVQEGEEGRIAVELATTGRVGEL
                     EGVLIDFTHPDLPPDEWWARPWWHVFGDGEHHCGIVFPGEAAAPKEDWDRAKADVQRK
                     PIYAVAGDTLKIGRVQQHNEVSNLHHMHDIRPRLAYLTLIHADGTEETEIGPLAFYGS
                     TRLIGHGAREAFDNAIARIAAIEPTGLAQTMFDSNRRPDWQNGADVVAKFYAAEAHDE
                     LFAMIDALNEDDTRGHVRLRSLVNAALLAGFALAQHEAERAFEEERRREERRKLATKA
                     RTDPDREICARTVWTEKPHLTTNAVAEIMMERWPEKFQAKDKSSLWKSINKFKPEPKG
                     "
     gene            5126..5323
                     /locus_tag="VN034_06465"
     CDS             5126..5323
                     /locus_tag="VN034_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV60276.1"
                     /translation="MSIKPIAVTTKDGFRMIPVSPAQGYRLIAAGELEIFKIGRATRI
                     TVASIEAYVARQLASRPQMAA"
     gene            5529..5789
                     /locus_tag="VN034_06470"
     CDS             5529..5789
                     /locus_tag="VN034_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60277.1"
                     /translation="MPIKASAATIAREILSVGVDNMLTATKTMTPREAALEILTNRYE
                     GLTWKACQFLGQCVGLPDRPLSEAQLRWLNKLKDEAGIGGEA"
     gene            5786..6070
                     /locus_tag="VN034_06475"
     CDS             5786..6070
                     /locus_tag="VN034_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60278.1"
                     /translation="MNRHDGGDLPHYRDSGLGYQAHSEASKDGARAALPKKPTQESRC
                     LDFIKERGRAGATSDVGYLSAQTAAGTVGKLLGLPVVDETQPKGGGNGQA"
     gene            6057..6809
                     /locus_tag="VN034_06480"
     CDS             6057..6809
                     /locus_tag="VN034_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60279.1"
                     /translation="MGKRNNRPNKSGRNENPTGRFARLPHEVMLSPAYRSLSPNARAL
                     LVEMMAMDNGQNNGSLWLSIRDAAARIGLVNKESVGRAFDDLIRAGLIAMTKEAHFSV
                     KAADTSRARCWRLTFVHWVGVGGRTDEWRQFVPADKAAARRAQMGLDADAAYRKALAR
                     EKLPVLNLRTTRDAAANIEAVAVHDLRTANADNDGKKPKSVGHDLRTHIAVTMGSRAN
                     ALCWREGKALKVLPDILPASWPDRAANPPLAA"
     gene            7315..7668
                     /locus_tag="VN034_06485"
     CDS             7315..7668
                     /locus_tag="VN034_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60280.1"
                     /translation="MSDDEELGGGWVDYLIASNEPADKALLGEFRDQAWAHGQLMQFI
                     ASDPANINAETYAKLEEGQEQENELQDRIRARGEALRAAGRSPCDEAAFKAWRASWVL
                     LASHLPSDDGPISHA"
     gene            7716..7949
                     /locus_tag="VN034_06490"
     CDS             7716..7949
                     /locus_tag="VN034_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60281.1"
                     /translation="MTYEPHEEWMELLAEASEGGAWTADPACGIAKQVVGQGVDSLSP
                     IQRRIFDREIWPTLRPMQIARDRERNIERVLSE"
     gene            complement(7970..8620)
                     /locus_tag="VN034_06495"
     CDS             complement(7970..8620)
                     /locus_tag="VN034_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HWV60282.1"
                     /translation="MCNLYTNKTTVQEMASLFGTPATYGSNAPEEVYPGYPGIVVREQ
                     DGTRVLQSMTWGFPVRLKGMKSTSKPKPVNNARDDKLLTPFWKHWFTTPAHRCLIPFT
                     AFAEAEGPKGKMTRTWISVEDQPTAAWAGLWRPSDEWGDCYTGVMVDATEELFDIHDR
                     MPVILHPDEHDAWLNCPAEDAIALVRKYPASRLVVDRTDAPWFKRKTTVSEAPTLI"
     gene            complement(8631..9341)
                     /locus_tag="VN034_06500"
     CDS             complement(8631..9341)
                     /locus_tag="VN034_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60283.1"
                     /translation="MNCADLNSLLTRIPRCRELDGGSHLRTDCLYPSSDPVMVFVKKQ
                     SFGDGFRVDDCGGAWRSAQRHGSPSVGMFDKASRRYSVNAESGMLWAESNNSEWLVPA
                     ILAVANASAMAARAALEMADRNEKSLNGQIQEVLMRHIPPHRIAKNYEYRGRSGHMWP
                     IDFAVTAERVTLIKSVVQNGNSINSTYAAFGDIGDDAPINKYAVFDRELKQDSTALIR
                     QVADLVPMKALEFSIMNA"
     gene            complement(9338..9859)
                     /locus_tag="VN034_06505"
     CDS             complement(9338..9859)
                     /locus_tag="VN034_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60284.1"
                     /translation="MADPTIDEVDAFIAGFKTLDGMLSEWEQKWGWDWQAQWGLLDVQ
                     GRQMGELKFSINAALTRPTIVATFRRKLIYRVDIVEQSDCKYNDLSAQDLGLPPIVCG
                     PHSHPWVENRRFVELNGFGELPVRKPVSIADTKIIRALEVVAADLNIHVEPRQRDVEL
                     PRQATLFAERISL"
     gene            10301..10642
                     /locus_tag="VN034_06510"
     CDS             10301..10642
                     /locus_tag="VN034_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60285.1"
                     /translation="MDVQAISFRGFKLVARGVSQAAVDRAREEVESVLEKHGVTAADL
                     DNLTHRYRILGESGVDVQQLRAEDYKRFNIDPTHVRANKRVFDVLNAARKLGATQPGS
                     IISFQPNQVHA"
     gene            10819..11136
                     /locus_tag="VN034_06515"
     CDS             10819..11136
                     /locus_tag="VN034_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60286.1"
                     /translation="MDSRLTIYGQEKLKPDVRDAALAAAHAVLSREGVTPAAAVQAAG
                     VDLLLAEGLEPDDRIDAHFREHGATLRAAEAHSVAVAAAQVEIARLDPDNAEFHVLFS
                     MSA"
     gene            11138..11434
                     /locus_tag="VN034_06520"
     CDS             11138..11434
                     /locus_tag="VN034_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60287.1"
                     /translation="MVYRKGERPVEKRVVDYSPDHPVASGIADGDHWLDAWLGQMCTP
                     WETITRKTGISRARIEELNDDAEPTPEEIGKLAALWWVTPEGLRRSIEEAKAYG"
     gene            11431..11877
                     /locus_tag="VN034_06525"
     CDS             11431..11877
                     /locus_tag="VN034_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60288.1"
                     /translation="MIFALLLAASVVPAGQTFDCTPTAVWDGDGPVWCSEGPHIRLSG
                     IAAREMDGTCRPGHPCPAADPVAARDHLVSLLGKRGGARPTGHISVSGPTMRCRSAGG
                     GGGGRTAAFCASPKSGDISCAMVRDGYAEKWDRYWQGHSCGSNRRP"
     gene            complement(12349..12621)
                     /locus_tag="VN034_06530"
     CDS             complement(12349..12621)
                     /locus_tag="VN034_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60289.1"
                     /translation="MNVITLTIDIGELASALADQLAQRGLVVPQQPEPADTDELWSAA
                     QVRGYLGIGQTTFHQRRKTPGFPSVVDRPGRPHWRASEMRAWAAGK"
     gene            12800..12946
                     /locus_tag="VN034_06535"
     CDS             12800..12946
                     /locus_tag="VN034_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60290.1"
                     /translation="MERLRAVILTPCVVGFFRFKVGVAVRLVHAINQGLNQSLKVRAG
                     QGRD"
     gene            complement(13125..13889)
                     /locus_tag="VN034_06540"
     CDS             complement(13125..13889)
                     /locus_tag="VN034_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60291.1"
                     /translation="MNRARNFPPSPSFAAIAVALATQIADLECFTGSDEETSPLVDAF
                     STTEKALTRRPIECIGDLLLKIEILSRIAEHSSVDPEEWQALARDVEQLNGPGMAFAP
                     EAWLRRWTAKGGGYVRTDTGLSFVAPEPPTFQQRLLLDELQRAQGHQAVADVIDGKSE
                     PVAPTWETARAAFDAKAAEIAQIEASDTHLDEYWDAWDTAMLCPSDSIDAANWKMAQF
                     FHWTDECGEFADEAAEKYRDAIFADVARHARKGGAA"
     gene            complement(13886..14203)
                     /locus_tag="VN034_06545"
     CDS             complement(13886..14203)
                     /locus_tag="VN034_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60292.1"
                     /translation="MQCFLAARIAEIEYDETVWTPADDAADWKVGRPIPQIITDEMER
                     FTDARLAAEDALIHTPTNDAANVRWKLAYCRIRYEDFCDFPDDWWQAIYADLERIAAG
                     GDA"
     gene            complement(14233..14958)
                     /locus_tag="VN034_06550"
     CDS             complement(14233..14958)
                     /locus_tag="VN034_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60293.1"
                     /translation="MTDDSTRAPDGATTLTTKDDATEAKAPTAFETALKDYTNIEAAR
                     EFWAGNDPSDKALGATAVGAFHAGWSAILEARAGAQMAVMEADAEAVAEVVAKLKFLH
                     AQDDIEDNCPADLWEMIRCVVADLERLTKRTPLQELMDANPPKVVFASSFVPCDNPMD
                     NLYLCEVSTGMCERECGVFRDLLTAAAYAAALSYKTGYPVHPYGAAVEYLENALQSPL
                     VRKKMASTWEHPKDAARAEGGAK"
     gene            15112..15333
                     /locus_tag="VN034_06555"
     CDS             15112..15333
                     /locus_tag="VN034_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV60294.1"
                     /translation="MTPTQSRMARAALKWSLTDLANKAGVGRATVARFELGDGETAAD
                     TIEKIRAALVNAGADFTRKAGRVGVTVSE"
     gene            15409..15585
                     /locus_tag="VN034_06560"
     CDS             15409..15585
                     /locus_tag="VN034_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60295.1"
                     /translation="MDPRDSLVAIYFLAAIFGVACCWLWFRSPKGKPMHEAGTAGALA
                     IGLASVGFILSQFL"
BASE COUNT         3134 a         4801 c         4932 g         3182 t
ORIGIN      
        1 cggccttgac cggcttggca gccttttcgg tcgccttgac cggcgagggg cgcgatttca
       61 ggccgaggcg gtgcgccttg ccgattaccg cattgcggct gaccccgccg agttcgtcgg
      121 cgatctggct ggcggtcagg cccttttccc acatgctgcg cagctgttcg atgcgctcgt
      181 cagtccatga catctaaaat tcctcatcat tccacgccgc gggccatatc gtccgtatcg
      241 cgatcctttc ggatcgatgt cgcgacggcc cagcttgcgg taagatcggg gaggcgatag
      301 gcgagaacgc catgaacgac cagccccaaa tttcgaaccc cgactcagcg aatatccgct
      361 caggtccggc tttcgccgaa cccggcgtcc cgcacatcca cacgctgaac gtgccgggca
      421 tgcaggccct atatgtcaag gaggtgcggc ggtttttcaa ggtccagctg caaacaatct
      481 gggctccggc gatcaccacg ctgctgttcc tcgtcatttt caccgtcgcg ctcggcggcg
      541 cgggacgcac ggtcatcggc gtgcccttcg ccgatttcat cgcgcccggc ctgatcatga
      601 tggcgatgct gcagaacagc ttcgccaatt cgagcttctc gctgctcgtc ggcaagatcc
      661 aggggacgat cgtcgattat ctgatgcctc cgctcgcggt cggcgaactg atcatcgcgc
      721 tcgtcggcgc cgcgatcacg cgcgcggtcc ttgtgggctt cgcgctctgg ctcgcgatgc
      781 tgctatggcc gggcgtccat gtcggcaccg atcatctgtg ggcggtcgct gttttcggcg
      841 tgctgggcgc gatgatgctc ggcttcctgg gcctgatcac ctcggtgtgg gccgaaaaat
      901 tcgaccatgc cgccgcggtg accaatttcg tcgtgacgcc gctcgcgttg ctgtcgggga
      961 ccttctattc ggtcgacaag ctggcgccct ggtttcagac gatcgcgcac ggcaatcccg
     1021 tctattatgc gatcatgggc tttcgctacg gtttcatcgg gacggtcgat tcgacgatcg
     1081 ccaatccggt gctgaccgca atgctcgtgc tcgtcgcggt caatatcctg cttggcgtga
     1141 tcacctatcg cctgctggcg tcgggctgga agctgaaggc ctgatcgcgc caaatttcgt
     1201 catcccggcc tccgccggga tgaagttctt attaatggcg gtggatcgcg cggacgcggt
     1261 agcggccgtg atcgagcccg tcgaacatcg catcgatctg cggatggtcg atcggtccgc
     1321 cgtcgccgtc ggcgacgaga ttctgctggc tgacataggc gatgtacgaa gaatcgccgt
     1381 tttcggcgag caaatgataa tagggctgtt ccttggcggg acggatttcc tcggggatcg
     1441 cctcatacca ttcgtcggtg ttcgcaaaca ccgggtcgat gtcgaacacg acgccgcgaa
     1501 agtcgaacat gcggtggcgc acgatgtcgc ccggcgcaaa gcgcgcgcgg ccgatgagcg
     1561 gcgcagtaac ggcggcggga agttcggaag aggactcggg tgtcatatga tcaatctatg
     1621 gctgtttacg cacgtttcaa gtccgttaca ttcgcactgg ccgcggtttg ccgaatcggc
     1681 tgtgatcagc gttacagatg cattggccat aagggctaaa ttgctcctct ggcttgtgca
     1741 tttcccgttc gggttgcggg ccgcaccttc aggggagaag cattatggct gacgtaccac
     1801 ccaatatctc cggacccgct tcgggcatcg tccagcgcgc gaaggatatc ctgctcaagc
     1861 ccaaggaaac ctggccggtg atcgcggccg agccggcgac gacgcagtcg atctatattc
     1921 cctatgtgct tgttcttgcg gcgatcggcc cgctcgccgg gctcatcggc ggccagctct
     1981 tcggtatcac gatactgggc gtaacctacc atccgccgct ggcgaccgcg ctggtttcgg
     2041 cgatcgtctc ttacgggctg tcgctcgcga ccgtcttcat ccttgcgctg gtgatcgacg
     2101 gacttgcgcc caatttcggc ggacagaagg atcaggtaca ggcgctgaag gtcgcggcct
     2161 attcggcgac cgccggctgg gtcggcggca ttttcgggat attgccggcg ctcgcggtca
     2221 tcggcctcct cttcgcgctc tacgggcttt atctgctgta tctcggtctg ccggtgctga
     2281 tgaaggcgcc gcaggacaag gcgctcggct acacggtcgt cgtggtgatc gtgtatatcg
     2341 tgcttttcct gatcgtcggc gccgtcgtcg gggccctcgc ggcgccgtcg ctcgtcacga
     2401 tcaggtgagc cgattatccg ctgccccggt ttcgcgccgg ggcagttggc tgtggtcgaa
     2461 catgaagaaa aatggaggag agtgagggaa cataaccctt actctctccg attatctgca
     2521 cctatcccga atcgtgagct tgcaaggcat tcccggctct ctatgttgtc ccggtgtatc
     2581 tgggtttatc ccggctgctc atcattcctg tgggaacgat tgagggaaca tggcgaagct
     2641 gaccgctaag accgtgctaa gcgccaaggc tggtcgccat gccgacggcg gcgggctgta
     2701 tctgctcgtc aagccgactg gcgcccggtc gtggctcctg cgcgtgcagg tcgatggtcg
     2761 ccgccgcgac attggccttg gttcggtcga aacctcggcg ctcgccatgc gcagcgatat
     2821 aggcaccgac ataccgcttg aagaaaagag ccgattgacg cttgccgaag cgcgggaact
     2881 gtcagcgcgg ttacgtaacg ccgccaaggc tggccgcgat cctgccgccg aacggaagaa
     2941 ggaccgaaag gccccgccgt cgttcaagga cgcggcgatt gccacacaca aggcgcagac
     3001 ggcatcgcat agctggtcag acaagacagc cggcgccttc ctgtcgtcgc tcgaaaccca
     3061 tgcctacccg acccttggca agatgcgggt cgatatggtc gaggccgaac atgtcgccaa
     3121 cgcgctcgcg ccgatctgga cggccaagcc tgacatggcg aaaaaggtcc gacagaggat
     3181 cgcaaccgtg ctggatttcg ccaaggcgaa gaactggcgt gcaacagagg cgccgcgcaa
     3241 ggctgtcggc gcgctcaccg gcaagacgaa gaagggcggc cacttcgagg cgatgcctta
     3301 cgcggaagtg ccgggctatt tccgaagcct gatgaacgaa accccgacca aggggcggct
     3361 tggcctgatg ctggcgattg ccaccgtggc tcgttcaatc gaggttcggg aggcgcgctg
     3421 gcagcacgtc gtaactgaca agcgggactg gaaccggccc gccgagctga tgcggaagaa
     3481 ccacgaagcg catacggtca cgctgaacga cgctgcgcta tgggtgctcg cgcacatcgc
     3541 cgaaatttca gacccgttga aaccggacac gctccttttg ccaaaccgga agggcaaacc
     3601 gatttcggac atgacgataa gcaaggtgat gcgtgacgcg ggcctgtcct ctgtgccgca
     3661 tggtttccgg tccagttttc gcgattgggc cgcggaacag catcccgaga taccggaccc
     3721 cgttgcggag gccgcgctat cgcatagcgt gcctgacgca gtgatcgccg cttataagcg
     3781 aaccaaattt ctagaaatgc gacggacgtt gttggatcat tggtccgcct tcatcctgag
     3841 cggtcggacg aacgtcgttc agcttgccga ccggcgagca tgagcaggcg aggcggctag
     3901 ggtagctccc gaacgtcggt tccgcaccgg ctgccgcgtc gttatcatcc ctgcggatcg
     3961 ccagcggagg cgaagatgac gaattatgca aaggtgccgg tgaggttcgg cggcgagatt
     4021 cgccgagtga ccgacctgca agttcaagaa ggcgaggagg gccggattgc cgtcgagctg
     4081 gcgacgacgg gccgcgtagg cgagcttgag ggcgtcctga tcgatttcac gcatcccgat
     4141 cttccgcctg acgaatggtg ggcgcgtccg tggtggcatg tctttggcga tggcgagcat
     4201 cattgcggca tcgtgtttcc gggcgaggca gcggcgccca aagaggattg ggatcgggcg
     4261 aaggcggacg tgcagaggaa gccgatctac gcggtggcag gagacaccct aaagattggg
     4321 agggtgcagc aacacaacga ggtcagcaat ctgcatcaca tgcacgatat caggccgcgc
     4381 ctagcgtatc tgacgctcat ccacgcagac ggcaccgaag agacggagat tggaccgctg
     4441 gcgttctacg gaagcacgcg gttgatcggg catggcgcgc gggaagcgtt cgacaacgca
     4501 attgcccgga ttgctgcaat tgagcctacc ggacttgctc aaacaatgtt cgacagcaat
     4561 cggcggcccg attggcagaa cggcgccgat gtggttgcca agttctacgc ggccgaagcg
     4621 catgacgaac tgttcgcgat gatcgatgca ttgaatgagg atgacacgcg gggacacgtt
     4681 cggctccggt cgctggtcaa cgcggccctg ctcgctggtt tcgcgctagc ccaacatgaa
     4741 gccgagagag catttgagga agagcggcgg cgagaagaac ggcgcaagct ggcgacaaag
     4801 gcccgcaccg atccggacag ggagatctgc gctcgcacgg tctggacgga aaagccgcat
     4861 ctgacgacta acgcggtggc ggaaataatg atggagcgtt ggcccgaaaa atttcaggca
     4921 aaggacaagt cctcgctctg gaaatcgatc aataaattta agccggagcc gaaaggctga
     4981 tcgcttgacg ttccagcgga tggtcaatga ttttcctccg ggtcttcaat gattttccgc
     5041 tggttggctg accaaagccg agcgtcacgg agataattcc agcgacaccc cgcgactggc
     5101 gggggacgca aatggagtta tcccaatgtc catcaagcct atcgccgtca cgacaaagga
     5161 cggcttccgc atgatcccgg taagccccgc acagggttac cgcctcattg ccgctggcga
     5221 gcttgaaatc ttcaagatcg gtcgcgcgac gcgcatcacg gtcgccagta tcgaagccta
     5281 tgttgcgcgc cagctcgctt cgcgtcctca gatggcggct taacagatgc gcggggcaca
     5341 agaaggggtg gcacccgata accgcgccgc tcggcctacg ctgagcctcg cccgcaaaga
     5401 ggtcgttgct gcgcttcccg cagacgtctc tccccatagc aaagaaggcc gccttgcgtg
     5461 tgccttcggg gcttggctca tcgacgctca agcggagtgg atgcaggcca acgggcgcaa
     5521 attcccccgt gccgatcaaa gcgagcgcgg cgactatcgc ccgcgaaatt ctttcggtgg
     5581 gagtcgataa catgctgacc gcaaccaaga cgatgacgcc gcgcgaggcg gccctagaaa
     5641 tcctcaccaa ccgatacgaa gggctgacat ggaaagcctg ccagttcctc gggcaatgcg
     5701 ttggcttgcc tgatcggcct ctgtcggagg ctcaattgcg ctggctgaat aagctcaaag
     5761 atgaagccgg gattgggggt gaggcatgaa ccgccacgac ggcggcgatc tgccgcacta
     5821 ccgcgacagc ggccttggct atcaggcgca cagcgaggcg tcgaaggacg gtgccagggc
     5881 ggcgcttcca aagaagccga cacaggaaag ccggtgtctg gacttcatca aagagcgggg
     5941 tcgtgcgggc gcgaccagcg atgttggcta tctctcggcg caaaccgcgg caggaacggt
     6001 tggcaagctg ctcggccttc ccgtggtcga cgagacgcaa ccgaaagggg gcggcaatgg
     6061 gcaagcgtaa caacaggccg aacaaatcgg ggcgcaatga aaacccgacg ggtcgctttg
     6121 cccgccttcc gcatgaagtg atgttgtcgc ccgcctatcg atcgctctcg ccgaacgctc
     6181 gcgctctgct ggtcgaaatg atggcgatgg acaacggaca gaacaacggc agtctctggc
     6241 tctcgatccg cgatgccgct gcccgcattg ggctggtcaa caaggagtcg gtcggcaggg
     6301 cttttgacga cctgatccgc gcggggctga tcgccatgac gaaagaggct catttctcgg
     6361 tgaaggcggc ggacacgtcc cgcgctcgct gctggcgcct gacgttcgtt cactgggttg
     6421 gcgtcggcgg tcgcactgac gaatggcgcc agttcgtgcc cgccgacaag gctgctgcgc
     6481 gccgcgcgca aatgggcctc gatgctgacg cggcataccg caaagcactg gcccgagaaa
     6541 aattgccggt cctcaatttg aggacaacga gggatgcagc ggccaatatc gaggctgtag
     6601 cggtccacga tttgaggacc gcaaacgccg acaatgacgg aaaaaagcca aaatccgtcg
     6661 gtcacgattt gaggacacat atagctgtta ccatgggcag tagggcgaac gctctttgct
     6721 ggcgtgaagg gaaggcgctc aaagtccttc ccgacatcct gcctgcctca tggcccgacc
     6781 gggcggcaaa tccaccgctc gcagcctgaa aaccgcaaag aattacagcc aaaataatcg
     6841 ggcgccgttt ttcgcgccaa ttttcagcac agattggggg aaacctatgg cacatctatc
     6901 tcaacggtcg ctgatcccgg cgctggatct gaaccggtgg ggaaggggtg gggaaacgtt
     6961 tccgagacgt tgtgcgacgg tcccgcgtta cggttcgcta ccgtcgtcgg ccccacatat
     7021 gttgacactg tcaatgtcac aattcccgga cgccagaaag cgcgcgcgcg ttagggggcc
     7081 agcacgttat ggtttgttat ggtcggggca atttctgtta gccgtaaaaa gcgcgccagg
     7141 acgaattttg tcaggcccta aaaagggcgg ttttctgccg tcagttttct aaagtagata
     7201 cgaatagata gtggctggcg cgctcgacca aaagggacat ctgtccgcgg acgcacccag
     7261 atttggttct atctcatggt gcgaagctta agtgacgatg atatgagcgt ctgcatgagc
     7321 gacgatgaag aactaggcgg cggctgggta gattatctca tcgccagtaa cgaaccggca
     7381 gataaggcgc tgctaggtga gtttcgcgat caggcatggg cgcacggcca gctcatgcag
     7441 ttcatcgcca gcgatcctgc gaatatcaac gctgagacct atgccaagct cgaagaaggg
     7501 caggaacaag aaaacgagtt gcaagaccgc atccgggcgc gcggtgaggc cctgcgcgct
     7561 gctggccgtt caccgtgcga cgaagctgcg ttcaaggcat ggcgggcttc atgggtgctg
     7621 ctcgcgtcgc acctgccatc tgacgacggg ccgatctccc atgcctgacg ccgccgtcag
     7681 taggcgaacg ttcgaatctc agctagggat gatccatgac ctatgaacca cacgaagaat
     7741 ggatggaact tcttgcggaa gcttcggagg gcggcgcatg gaccgctgat cccgcatgcg
     7801 gcatcgccaa acaggtggta ggccaaggcg tcgatagcct cagccctatc cagaggcgga
     7861 tattcgaccg ggagatttgg ccgacgctac gtcctatgca gatcgcgcgg gacagagaac
     7921 ggaatatcga gcgcgtgtta agcgagtgat ttagcgcgcg gtggtccccc taaatcagag
     7981 tcggcgcttc cgacactgtc gtttttcgct tgaaccacgg cgcgtcggtg cgatcgacga
     8041 ccaacctgct tgccggatat ttccgcacta gcgcgatcgc atcctcagcg ggacaattca
     8101 gccacgcgtc gtgctcgtca ggatgcagga tcaccggcat ccggtcgtga atgtcgaaca
     8161 gttcctcagt cgcatcgacc atgacgccag tatagcagtc gccccattcg tcggacgggc
     8221 gccatagtcc ggcccatgcc gcggtgggct gatcttcaac gctgatccat gtccgcgtca
     8281 tcttcccttt cggcccctcg gcctcggcga aggcggtgaa tgggatcagg cagcgatgtg
     8341 ccggcgtcgt gaaccaatgc ttccagaaag gtgtcagcag cttgtcgtcg cgcgcgttgt
     8401 tgaccggctt cggcttgctg gtcgacttca tgcccttgag gcgaacggga aagccccaag
     8461 tcatcgattg taggacgcgc gtgccatcct gctcgcggac aacaatgccg ggatagccgg
     8521 gatagacctc ttcgggcgcg ttagagccgt aggtggctgg cgttccgaaa aggctcgcca
     8581 tctcctgcac cgtcgtcttg ttggtgtaga ggttgcacac ccggggagga tcacgcgttc
     8641 ataatgctaa attccagtgc cttcatcggc accaaatcag cgacttgtcg gatgagcgcc
     8701 gtgctgtctt gcttcaattc acggtcgaaa acagcatatt tattgatcgg cgcatcgtcg
     8761 ccgatatcgc caaatgcggc gtaggtggaa ttgatgctgt tgccattctg aacgaccgat
     8821 ttgattaggg tcacccgctc agcggtgacc gcgaagtcga tcggccacat gtggcccgag
     8881 cgcccccgat actcgtagtt ctttgcgatg cggtgtggtg ggatgtggcg catcaaaacc
     8941 tcttgaattt ggccattcaa ggacttttca tttcgatcag ccatttcaag cgcggcacgc
     9001 gcggccatcg cggacgcgtt ggccaccgcc aaaattgcag gaaccagcca ttcgctgtta
     9061 ttggattcgg cccatagcat cccgctttct gcgtttaccg aatagcgcct gctggcctta
     9121 tcgaacatgc cgaccgatgg gcttccgtgt cgttgagcgg accgccatgc gccgccgcag
     9181 tcatcgacgc ggaacccatc accaaagctc tgttttttca caaacaccat cacgggatct
     9241 gacgacggat aaagacaatc ggtgcgcaga tgcgaaccgc cgtccagttc tcggcatcgc
     9301 ggaatccgag tcagcagaga attgaggtct gcacagttca cagcgatatc ctctctgcga
     9361 aaagcgtcgc ctgacgaggc aactccacgt ccctttgcct cggctcgaca tgaatgttca
     9421 aatcggctgc gacgacttcc aatgctctta taatcttcgt gtcagcgatt gaaacaggct
     9481 ttcggaccgg cagctcgcca aacccgttca actcaacgaa tcgtcggttc tccacccaag
     9541 gatgggagtg cgggccgcac acgatcggtg gcagcccaag atcctgcgct gaaaggtcat
     9601 tatatttgca gtcggactgt tccacgatgt cgacccgata gatgagtttc cgtcgaaatg
     9661 tcgctactat cgtcggccga gttaaggccg cgttgatact aaacttcaac tcacccatct
     9721 ggcgcccttg gacatcgagc agaccccatt gtgcctgcca gtcccatccc cacttttgct
     9781 cccattctga aagcatgccg tcgagggtct taaaccccgc aataaaagca tcgacttcgt
     9841 caatagttgg gtcagccata gtgcacggcg taaatctcgg atcgcgcgga gtcgagtcgg
     9901 aaggcaatca cgaaaaagct ctagtcacct tcgcctacgc aactgccgtt tccattcgat
     9961 aatgtccggc atgggcaatc gccgggtggg gtcttcggaa gttagttcca gcatcgctag
    10021 cgtccgcttc cgcttcgcat agtagcattg caggcagcgc atccgcttgc gcaccgcagg
    10081 cagcttgtcg ttccatcctt tccgttcaaa cagataccag agcgcgtgcg gatcgaagat
    10141 gccgacgttt ccgcaatggc acgacgcctt gatggtccga tgccggacgg cggcatcgaa
    10201 caggccgctg atagggttcg ctagctctcg actcatgact cgacaaatga gaacattggt
    10261 ggaacatttg tgcaatcgag aatcgagtcg gagaatgaaa atggacgtgc aagcgatcag
    10321 ctttcgcggc ttcaaacttg tcgccagagg cgtcagccaa gcggccgttg atagggcgcg
    10381 tgaggaggtc gagagcgtgc ttgaaaagca cggcgtcacc gcggccgatc tggacaatct
    10441 cacccatcgc taccgcattc tgggcgagtc cggcgtcgac gtgcagcagc ttcgggccga
    10501 agattataag cgcttcaaca tcgacccgac gcacgtccgc gcgaacaagc gtgttttcga
    10561 cgtgctcaac gccgcccgga aattgggcgc gacccagccc gggagcatca tctcgttcca
    10621 gcccaaccag gtgcacgcgt gacgccggga cacaatggaa agcccgatgc cgaccgcatc
    10681 gcggactatc ttgaaggcaa ggcagacaga ttcaacgatc gcgctggacg gacgggcgat
    10741 gctgagctgc gagcccgtgc cacgacatta aacgcggcgg catccgacat tcgcgcgcgt
    10801 ctgttcgagg attgaatgat ggacagcagg ctcacgatct acggtcagga aaagctgaaa
    10861 cccgacgtgc gggatgcggc gcttgccgct gcccatgccg ttctttcgcg cgagggcgtc
    10921 actccggccg ccgcggtcca ggctgccggc gtcgatttgc tgctcgcgga aggactggag
    10981 ccagacgacc gcatcgacgc tcatttcagg gagcacggcg ccacgctgcg cgctgccgaa
    11041 gcacatagcg tcgccgtggc tgcggctcag gtagagatcg cgcggctcga tcccgacaat
    11101 gcggagtttc acgtcttgtt ttcgatgagc gcctgacatg gtctaccgga agggcgaacg
    11161 cccggtcgaa aaacgggtcg tggattattc gccggaccat cccgtcgcca gcggcatcgc
    11221 ggacggcgat cactggttgg acgcatggct cgggcaaatg tgcacgccgt gggagacaat
    11281 cacacgcaag accggcatct cgcgcgcccg gatagaggaa ctgaacgacg atgcggagcc
    11341 gacacccgaa gagatcggga agttggctgc tttatggtgg gtgacaccgg aagggctgcg
    11401 gaggtcgatc gaagaggcta aggcctacgg atgatcttcg cccttctact cgccgcttcc
    11461 gtcgttcccg ccggccagac cttcgactgc accccaacgg cagtatggga tggcgacgga
    11521 ccagtctggt gctcggaggg gccgcatatc cgtctttcag gcattgccgc gcgcgagatg
    11581 gatggaacgt gccgccctgg gcatccgtgt cccgctgccg atcccgtcgc tgcccgtgat
    11641 caccttgtgt cgctgctcgg aaagcgcgga ggtgcgcgcc cgacgggtca catatcggta
    11701 agcggtccca cgatgcgctg tcgatcagcg ggcggcggtg gaggtggccg aacggctgcc
    11761 ttctgcgcgt cgcccaagtc cggcgatata tcctgcgcaa tggtgcgtga cggctatgca
    11821 gaaaagtggg accggtactg gcaaggtcac agctgtggga gtaacaggcg accttgaggc
    11881 caatggtcgt agtcatgcgt tgcgacacag ctcctcgatc gcttcacgcg tcaaatgcgg
    11941 tttgcgattg cccgaatttt caagcagcag acatcttgac cgttccagcc gcgccctcgc
    12001 ttcggacttt gtctcccgat tgacgatatc ggttaaggtg tccaaaatcc cgaagtcgac
    12061 aagctcgcag acttgatccg cagtgaaact gattccagtc tcattccgga gtgcgcgctc
    12121 gacctttctc agaattgttt cggctgacat cacgctatct cccagtttga aagtgcaaca
    12181 tgcgagacgg ttgtaagctc ccatctacgc cgggagcgtc gcctggcgaa gcaaaagccc
    12241 agtatatagc cccgaactgc cgcaacctga gcaggggcga agcaaaaata agggcggcaa
    12301 gattcccctg ccaccctccg atgagcgcat cgcccacccg ctttaacgtc acttcccggc
    12361 agcccacgcc cgcatctcac tcgctcgcca atgtgggcgc cccggtcggt caacgacgct
    12421 cggaaagccc ggtgtcttgc ggcgctgatg gaacgttgtt tgaccgatgc cgagatagcc
    12481 gcggacctgc gcggcgctcc acagttcgtc cgtgtccgca ggttccggct gttgcggcac
    12541 gacaagcccg cgttgagcca actggtcggc aagggcgctg gccagctcgc caatgtcgat
    12601 ggtaagggtt atcacgttca ctctcacttc ctcctatcgt gaagggtgat atccctaagg
    12661 acgcgcctgg ctgtaagcca cctcgcgctc aagcctcgat gctccacgca ggggccacgt
    12721 cgctgcgctc ccaatcgcaa cgatcatcct cgcagtcgtc gtcgttggca gggtcgctta
    12781 tggggcaacc ggggccgtcg tggaacgtct gcgagcagtc atcctcaccc cctgtgtggt
    12841 cggtttcttc cggttcaagg tcggtgtcgc cgtccgcctc gtccatgcga tcaatcaggg
    12901 attgaaccag tcgctcaagg tgcgcgcggg gcaaggacgg gattagggac agggcgatgt
    12961 cggcggggag ccgcgaagcc ccccgaccga tcctgataga cagcgcgctc acggcagcac
    13021 ctgaaaagcg aggtccacgg cctgaattgc cagccacgac gtgccgagac agacagccca
    13081 accccatgcc gtcatagcgg ggctgctcgg ttcgcgacgg gcgctcatgc cgcaccgcct
    13141 ttccgcgcat ggcgggcgac gtccgcaaag atcgcgtccc ggtatttctc ggcggcttcg
    13201 tcggcaaatt ccccgcactc gtctgtccag tgaaagaact gcgccatctt ccaattggcg
    13261 gcgtcgatac tgtcggacgg gcaaagcatc gccgtgtccc acgcatccca atattcatcg
    13321 agatgcgtat cgctggcctc gatttgagca atctccgctg ccttggcatc gaatgccgcg
    13381 cgcgccgttt cccatgtcgg cgctaccggc tcgctcttgc cgtcgatcac gtcggcgacg
    13441 gcttgatgac cctgcgcccg ctgcaactca tccaacaaca ggcgctgctg gaacgtcggc
    13501 ggctccggtg ccacaaacga cagccccgta tcggtgcgga cgtagccgcc gcccttggcg
    13561 gtccagcggc gcagccacgc ctcgggagcg aaggccatgc cggggccatt cagttgttcg
    13621 acgtcacgag cgagcgcctg ccattcctcc gggtcaacgc tgctatgctc tgcgatccgg
    13681 gagaggattt cgatcttgag cagcaggtcg cctatgcact cgatagggcg ccgggttagc
    13741 gccttctcgg tcgtgctgaa tgcgtcaacg agcggggagg tttcctcgtc cgatcccgtg
    13801 aagcattcga ggtcggcaat ctgcgtcgcc agcgcgacgg caattgcggc gaagctgggc
    13861 gacggcggga agttgcgagc gcggttcatg catcacctcc cgcggcgatc cgctcaagat
    13921 cggcatagat cgcctgccac caatcatccg ggaagtcgca gaagtcttcg tagcggatac
    13981 ggcaataggc gagcttccac ctgacattgg ccgcatcgtt ggtcggcgtg tgaatcagcg
    14041 catcttcggc agcgaggcgc gcatcggtga accgttccat ttcgtcggta atgatttgcg
    14101 gtatcggacg gccaaccttc cagtcggcag catcgtcggc gggcgtccat accgtttcgt
    14161 catattcgat ttctgcgatg cgggcggcga ggaagcactg catcgagtgc tcccatagga
    14221 tagcgggcac gttcatttcg cacccccttc cgcacgcgcc gcgtccttgg gatgttccca
    14281 agtgctcgcc atcttcttgc ggacgagcgg cgattgcagg gcattttcga gatattcgac
    14341 agctgcccca taaggatgga ccggatagcc cgtcttgtag ctgagcgcgg cggcataggc
    14401 ggcggcggtg agcaagtcgc gaaacacgcc gcattcccgc tcgcacatgc cggttgagac
    14461 ttcgcagaga tagagattgt ccatcggatt atcgcacggc acgaacgacg acgcgaaaac
    14521 caccttgggc gggttcgcgt ccatcagctc ttgcagcggc gtccgctttg tcaggcgctc
    14581 cagatcggcg acgacgcacc ttatcatttc ccataggtca gcggggcaat tgtcctcgat
    14641 gtcgtcctgc gcgtgcagga acttcaactt cgcgacgacc tccgccacag cctcggcgtc
    14701 ggcctccatc accgccattt gcgccccggc gcgagcttca aggatggcgg accatccggc
    14761 atggaaagcg ccaacggcag tagcgcccaa ggctttatcg ctcgggtcgt tcccggccca
    14821 aaactcgcgg gccgcctcaa tgttcgtgta gtctttcagc gccgtctcaa atgcggttgg
    14881 ggccttcgct tcggtcgcgt cgtctttagt ggttaaggtg gtggcgccat cgggcgcacg
    14941 ggtgctatca tccgtcatag tcgtttccct gtgtaatagg gtttcgatca gcgccggggt
    15001 tgggattgcc gtcccgtcct cggcgcgcct cggcttctgg ccttggcgat aacatcgtta
    15061 tcatggttaa agcgattgcg tcaatgatgt tgtgagacta ttgttatcgc gatgacaccg
    15121 acacaatcac gaatggcgcg cgccgctctc aaatggtccc tgaccgatct agcaaataaa
    15181 gccggggtcg gtcgcgctac tgtcgcgcgc ttcgaacttg gcgacggcga aaccgctgcc
    15241 gatacaatcg agaagatcag ggccgcgctc gttaatgctg gtgccgactt cacccgcaag
    15301 gctggccgcg tcggggtgac tgtttccgaa tgagcttggc cgttgtctca tagcggcacc
    15361 ctcggccaat cttgacgcgc tcgtgccgtt cggcgacaag ctgcgcctat ggacccgcgc
    15421 gacagcctcg ttgcaatcta cttcctcgcc gcgatcttcg gcgtggcttg ctgctggctg
    15481 tggttccgca gccccaaggg caagccgatg catgaagcgg ggaccgctgg cgcactggcg
    15541 atcggtctag ccagcgtcgg gtttatcctc tcgcagttcc tgtaggctcg gatcggcccg
    15601 aaatcggggc aggggattcg atctgaggca tcagggctac atgggtagcc tttcgtcctg
    15661 atcgcgctct acggtgcctc tacgggcaaa tttcggcatg gccgttttat aacggtggat
    15721 aacgggctta tctgcacggt tccccggaag cgcgcgcgat atgttaaggg ggcgaggcga
    15781 atttctttac gccatgacgc gcgcgttacc cgcgatagat taagggcgga tcggcttggc
    15841 tgcgcagata ccgcacgggc gcccgcgtta ggggctgtcc tcggaatcgg acaactacgc
    15901 gagcgcacac gttataggat cgggccaatt tcagcagtat tacgcgcacg cgcgttaggg
    15961 gaaatgccac cagtacgcac aggcgcgcgc gttagggcga gtgaaaatat tcggacaccg
    16021 taaaaagggc cggtgccgac cctgattat
//
LOCUS       DATMSU010000246         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_146485,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000246 DATMSU010000000
VERSION     DATMSU010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146485"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VN034_06565"
     CDS             <1..682
                     /locus_tag="VN034_06565"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWV60296.1"
                     /translation="GVGSRRDVERMIEEGRIALNGEVVAQPAPLLSSLDGLTVDGNPV
                     AKPVSTRLYRFHKPAGCLTAARDPKGRKTIYDLLPKGLPRLMPVGRLDYNTEGLLLLT
                     NDGEFKRQLELPASGVERTYRARAFGDISQAQLEELAMGIEIDGVRYGKIDANLERRT
                     GRNQWVEMTLTEGKNREVRKVLEHFGLQVSRLIRTRYGEFTLADLDMGAVDVVPRDEL
                     FKFRRRLG"
     gene            682..1227
                     /gene="rsmD"
                     /locus_tag="VN034_06570"
     CDS             682..1227
                     /gene="rsmD"
                     /locus_tag="VN034_06570"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWV60297.1"
                     /translation="MRVISGEWRGRKLLAPKNDATRPTADRTRETLFSMLTSRLGSFE
                     GLYVADLFAGSGALGIEALSRGAEQCLFAEQDRDALDALRKNLGALGATPRADIRAGS
                     VLALGPARRSYDLLMFDAPYATGAGSVALDKLNRLGWIGPDSWISVETADKEAVDVAG
                     FTIDTARKVGKAKLTLLRQAV"
     gene            complement(1211..2476)
                     /locus_tag="VN034_06575"
     CDS             complement(1211..2476)
                     /locus_tag="VN034_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60298.1"
                     /translation="MAPYRQHRRILTASLVGTAVEFYDFYIYGTAAALVFGPLFFPES
                     SASAQLMYSFMTFGLAFFARPVGAIVFGHYGDRIGRKSTLVASLMLMGASTLLIAFLP
                     TYAMVGWIAPLLLCILRFGQGFGLGGEWGGAALLAVENAPAGWKSRFGMFPQLGAPVG
                     FIAANGLFLLLGLGLNDADFAAWGWRIPFLLSAVLVGLGLWVRLKIGETPDFAKAVAQ
                     EAPVAVPLGELFRHHLVATLAGTFAVVACFAIFYLATSFALAHGTTALGYPKEQFLLI
                     QLGAILFMAVGIIVAGYWSDSSSAQRVLSWGCGATILIGFLFGPSLASESWPLIFLGL
                     AAALFVMGLVYGPLGSWLTGLFPVRVRYTGASVAFNAGGILGGAMAPIIAQALAERGG
                     TETVGYYLALAGIVSWLGLLMVRRITPPA"
BASE COUNT          506 a          925 c          860 g          359 t
ORIGIN      
        1 tggcgtcggc tcgcgccgcg acgtcgaacg gatgatcgag gaaggacgca tcgcgctcaa
       61 cggcgaggtc gtggcccagc ccgcaccgct gctgtcgtcg ctcgacgggc tgacggtcga
      121 cggcaatccg gtcgccaagc ccgtttcgac gcgcctctat cgcttccaca agcccgccgg
      181 ctgtctcacc gcggcgcgcg atcccaaggg ccgcaagacg atctacgacc tcttgcccaa
      241 agggctgccg cgcctgatgc ccgttgggcg cctcgactat aataccgagg ggctgctgct
      301 gctcaccaac gacggcgagt tcaagcgcca actcgaattg cccgcgagcg gagtcgagcg
      361 cacctaccgc gcccgcgcct ttggcgacat cagccaggcg cagctcgagg agctggcgat
      421 gggaatcgag atcgacggcg tccgctatgg caagatcgac gccaatctcg aacggcgcac
      481 cgggcgcaac cagtgggtcg aaatgacgct gaccgagggc aagaaccgcg aagtgcgcaa
      541 ggtgctcgaa catttcggat tgcaggtcag ccgcctgatc cggacgcgct atggcgaatt
      601 caccctcgcc gacctcgaca tgggcgcggt cgatgtcgtg ccgcgcgacg aacttttcaa
      661 attccggcgg cgtctcggct gatgcgcgtg atttcgggcg aatggcgcgg gcgcaagctg
      721 ctcgcgccga agaatgacgc cacccgcccc accgccgacc gcacgcgcga aacgcttttc
      781 tcgatgctca cgagccgtct cggcagcttc gaagggctct atgtcgccga tctcttcgcg
      841 ggctcggggg cgctcgggat cgaggcgctg tcgcgcggcg ccgaacaatg cctgttcgcc
      901 gagcaggatc gtgacgcact cgacgcgctg cgcaagaatc tcggcgcgct gggagcaacg
      961 ccgcgtgccg acatccgcgc gggatcggtc ctggcgctcg gccccgcccg gcgcagctac
     1021 gacctgctga tgttcgatgc gccctatgcg accggcgcgg gcagcgtcgc gctcgacaag
     1081 ctgaaccgcc tcggctggat cggccccgac agctggattt cggtcgaaac cgccgacaag
     1141 gaagcggtcg atgtcgcggg ctttacgatc gacacggcac gcaaggtcgg caaggcgaag
     1201 ctgacgctgc tcaggcaggc ggtgtgatcc gccgcaccat cagcaggccg agccagctca
     1261 cgatcccggc gagcgccaga taatagccca cggtttctgt cccgccgcgt tcggccaatg
     1321 cctgtgcgat gatcggcgcc atcgcgccgc cgaggatgcc gcccgcgttg aacgccaccg
     1381 acgcccccgt atagcgcacg cgcaccggaa acagcccggt cagccagctt ccgagcgggc
     1441 cgtagaccag ccccatcacg aatagcgccg cggccagccc gaggaagatc agcggccagc
     1501 tttccgatgc caacgagggg ccgaacagga aaccgatcag gatcgtcgcg ccgcaacccc
     1561 agctcagcac gcgctgcgcg ctcgagctgt cgctccaata gccggcaacg atgatcccga
     1621 ccgccatgaa caggatcgcg ccaagctgga tcagcaggaa ctgctccttc ggatagccga
     1681 gcgccgtcgt cccgtgcgca agcgcgaagc tggtcgcgag gtagaagatc gcgaaacagg
     1741 cgacgaccgc gaaggtcccc gcgagcgtcg cgaccagatg gtgccggaac agctcgccga
     1801 gcggcaccgc gacgggcgct tcctgggcaa cggctttggc gaaatcgggc gtctcgccga
     1861 tcttcaatcg cacccatagc cccagcccga cgagcaccgc cgacaggagg aacggaatgc
     1921 gccagcccca cgcggcgaaa tcggcgtcgt tcagtcccag ccccagaagc aggaacaggc
     1981 cgttcgcggc gatgaagccg accggcgcgc ccaattgcgg gaacatgccg aagcgcgatt
     2041 tccatcccgc cggcgcattc tcgaccgcga gcagcgccgc gccgccccat tcgccgccga
     2101 gcccgaagcc ttggccgaag cgcaggatgc agagcagcag cggcgcgatc cagccgacca
     2161 tcgcataggt cggcaggaag gcgatcagca aggtcgaggc ccccatcagc atcagcgacg
     2221 cgacgagggt cgacttgcgc ccgatccggt cgccataatg gccgaacacg atcgcgccga
     2281 cgggccgcgc gaaaaaggcg agcccgaagg tcatgaagct gtacatcagc tgcgccgacg
     2341 ccgaactctc gggaaagaag agcggcccga acaccagcgc cgccgcggtg ccatagatat
     2401 agaaatcata aaattcgacc gcggtgccga cgaggctcgc ggtcaggatg cgccgatgct
     2461 gtcgataggg tgccagatcc acgggcaacg cccctcccca ttattgctcg cgacgctttg
     2521 ggcgcgcgtc ccgccgggcg caagcatgaa tgcgaagcgt cagcggctcc cggcggcaag
     2581 ttgcgcgcgg agacgcagca gctcgcgctc ggacacccgc gacagcgacg ccgcgggctt
     2641 gcccttgcgc
//
LOCUS       DATMSU010000247         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_184394,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000247 DATMSU010000000
VERSION     DATMSU010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184394"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1112
                     /locus_tag="VN034_06580"
     CDS             24..1112
                     /locus_tag="VN034_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWV60299.1"
                     /translation="MEPPRPLPPIAGRGAPTNLRPTRTGSLDREADGDWLDAAEDIDG
                     EAPRLHTTVTVEHPKTIIARNSSPDVGFDRSINPYRGCEHGCIYCFARPTHAFHDLSP
                     GLDFESRLFAKPDAAKLLRAELAKRNYKVAPLAIGTNTDGYQPIEREWGITRSVVEVL
                     AETRHPLIITTKSDRVLRDIDLLADMARDNLVGVAVSVTSLDPKIARTLEPRAPHPRR
                     RLAAIRKLIDAGVPTQVNISPIIPAITDHEIEAIMAAAAEAGAIRASYILMRLPYEVA
                     PLFRAWLAAHYPDRADKVMHMVQDIRGGRDNDAQFFTRMRGHGVWAQLIRTRVKRAAR
                     EHGMDRSFPPIRSDLFRPPERDGQMELF"
     gene            complement(1296..2900)
                     /locus_tag="VN034_06585"
     CDS             complement(1296..2900)
                     /locus_tag="VN034_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWV60300.1"
                     /translation="MGMQGQPLLVTSLIDHAAREHAGREIVSRWADGSMTRSNWGEVG
                     ADARRFAAAMVKLGMKKGDRIATLAMNHGHHLVSWYGTAGMGGVLHTVNPRLFDEQLI
                     YIVNHAEDRVLLFDAMFLPIVERLRPQLPTVEHFILFDAPAREGYLSYRDLIDAEDGS
                     FQWIALDERDPVGLCYTSGTTGNPKGVLYEHRSNVIHAITEIQPDVFDMSNRSVILPI
                     VPMFHANSWGIPFAAATVGAKLVFSATNDAQVLCDLMHDEGVTHSAGVPTVWIAMFAH
                     MDATGIGYGKLHRVIIGGSACPRAMIERFMKAGIDVGHAWGMTETSPIGTMGKRPWNW
                     DEMSFDERVDIVARQGCPPFGVELRIVDDEDKDLPRDGETSGRLQCRGPWIIQRYFKA
                     DADAADADGWFDTGDVAVLHPDGVMQITDRAKDVIKSGGEWISSIELENAAVGAPGVQ
                     EAAAVGVYHPKWDERPILLIVKKPGAEVSEASVLDYLADKVAKWWLPDEIVFVDELPH
                     TATGKILKRQIRDDYKDYRLRSLAEA"
     gene            complement(2980..>3800)
                     /locus_tag="VN034_06590"
     CDS             complement(2980..>3800)
                     /locus_tag="VN034_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWV60301.1"
                     /translation="ALVGAQASWAQEPQDSSAHERSTGQGGLFGGDVAIAPALQLPAA
                     EPWTLAERMTREKDAFGFYFSSHPVEQYEAIVSARGARSYGDICDNVEMSPGTRIPMV
                     MAAMVESARPRVSQRGNRFLNLTLSDRSGQFQSSCFDEIAGKTLEALAADGGCAMLSV
                     ELDLLEGEETPRVTVRGAQSLADMAATAALQLTCRVDMPAAIEDMARLLVKRDDAKGR
                     VIVTTIDPLTDDEIRIELGRGFALGPDTVSRLDMIAGVTQPALSLVAPREFRAR"
BASE COUNT          659 a         1326 c         1156 g          659 t
ORIGIN      
        1 catcgacacg ctattctgtg ccgatggaac cacccaggcc cctgcccccg atcgcgggcc
       61 gcggcgcccc aaccaatctg cgcccgaccc gcaccggatc gctcgaccgc gaggccgatg
      121 gcgactggct cgatgccgcc gaggatatcg acggcgaagc gccgcggctg cacacgacgg
      181 tcaccgtcga gcacccgaaa accatcatcg cgcgcaattc ctcgcccgat gtcgggttcg
      241 accgctcgat caacccttat cgcggctgcg agcatggctg catctattgt ttcgcgcgcc
      301 cgacccacgc ctttcacgat ctgtcgcccg gtctcgattt cgaaagcagg ctcttcgcca
      361 agcccgacgc ggcgaagctg ctgcgcgccg aactcgccaa gcgcaattac aaggtcgcgc
      421 cgctcgcgat cggtaccaac accgacggct atcaaccgat cgaacgcgaa tggggaatca
      481 cgcgttcggt ggtcgaggtg ctggccgaaa cgcgccatcc gctgatcatc acaaccaagt
      541 ccgaccgcgt gctccgcgac atcgacctgc tcgccgacat ggcacgcgac aatctggtcg
      601 gtgtcgcggt gtcggtgacc tcgctcgatc caaaaatcgc gcgcacgctc gaaccgcgcg
      661 ccccgcatcc gcgccgccgc ctcgccgcga tccgcaagct gatcgacgcc ggggtgccga
      721 cgcaggtcaa tatctcgccg atcatccccg cgatcaccga tcacgagatc gaggcgatca
      781 tggcggccgc cgccgaagcc ggcgcgatcc gcgcctccta catattgatg cgcctgccct
      841 acgaggtcgc gccgctcttc cgcgcctggc tcgcggctca ttatcccgac cgcgccgaca
      901 aggtgatgca tatggtgcag gatatccgcg gcggccgcga caacgacgcc cagttcttca
      961 cgcgaatgcg cggccacggc gtctgggcgc agctcatccg cacccgcgtc aaacgcgccg
     1021 cgcgcgaaca tggcatggac cggagcttcc cgccgatccg cagcgacctg ttcaggccgc
     1081 cggaaagaga tgggcagatg gagctgttct aagtcccctc ccccttgatg ggggaggcag
     1141 cgagacttgg cagcttgctg cctagtcaca gcggtggggg tgcgttctcg gtctgaaata
     1201 gacggcgcaa ggacgcacca tcaccccaat ccaacttcgc ctaatcgcct ccggtgataa
     1261 ggcagcgtat ccttccccca tcaaggggga aggagttacg cctccgctag cgaccgcaac
     1321 ctataatcct tataatcgtc gcggatctgc cgcttcaaaa tcttgcccgt cgccgtgtgc
     1381 ggcagttcgt cgacgaaaac gatctcgtcg ggcagccacc atttggcgac cttgtccgcg
     1441 agataatcga gcacgctcgc ctcgctcacc tcggcgcccg gctttttcac gatcagcagg
     1501 atcggccgtt cgtcccattt ggggtgatag acgccgaccg ccgcggcttc ctgcactccg
     1561 ggcgcgccga ccgcggcgtt ttcgagctcg atcgagctga tccattcgcc gcccgacttg
     1621 atcacatcct tcgcacggtc ggtgatctgc atgaccccgt cggggtgcag caccgcgacg
     1681 tcgccggtat cgaaccagcc gtccgcatcg gccgcgtcgg catcggcctt gaaatagcgc
     1741 tggatgatcc acgggccgcg gcactgcagc cgcccgctcg tctcgccgtc gcgcggcaga
     1801 tccttatctt cgtcgtcgac gatgcgcagt tcgactccga acggcgggca accctgccgt
     1861 gcgacgatgt cgacgcgctc gtcaaagctc atctcatccc aattccacgg ccttttgccc
     1921 atcgttccga tcggcgaggt ttcggtcatc ccccaggcat ggccgacatc gatccccgcc
     1981 ttcatgaacc gctcgatcat cgcgcgcggg cacgccgacc cgccgatgat cacacggtgc
     2041 agcttgccat agccgatgcc cgtcgcgtcc atatgcgcaa acatggcgat ccacaccgtc
     2101 ggcacgccgg cgctgtgggt gacgccctca tcgtgcatca ggtcgcagag cacttgcgcg
     2161 tcgttggtcg ccgaaaacac cagcttcgcg ccgacggtcg cggcggcaaa gggaatgccc
     2221 cagctgttcg cgtggaacat cggcacgatc ggcaggatca cgctgcggtt cgacatgtcg
     2281 aacacgtcgg gctggatttc ggtgatcgcg tggatgacgt tcgagcggtg ctcgtagagc
     2341 acacccttcg ggttgcccgt cgtcccgctg gtatagcaaa gcccgacggg atcgcgttcg
     2401 tcgagagcga tccattgaaa gctgccgtcc tcggcatcga tcaggtcacg ataggaaaga
     2461 tagccctcgc gcgccggggc gtcgaacagg atgaaatgct cgaccgtcgg caattgcggg
     2521 cgaagccgct cgacgatcgg caggaacatc gcgtcgaaca gcagcacgcg gtcctccgca
     2581 tgattgacga tatagatcag ctgttcgtcg aacagccgcg ggttcaccgt gtgcagcacg
     2641 ccgcccatgc cggcggtgcc gtaccagctg accagatggt gaccatggtt catcgccagc
     2701 gtcgcgatac ggtcgccctt cttcatcccc agttttacca tcgcggcggc aaagcgcctc
     2761 gcgtctgcgc cgacttcccc ccagttcgat cgcgtcatgc tgccgtcggc ccatcgcgac
     2821 acgatctcgc gtcccgcatg ttcgcgcgcg gcgtgatcga tcaggctcgt gacgagcagc
     2881 ggctggccct gcattcccat ggtgcggcat cctcaaatca tatatcgttg ccggcaatgg
     2941 atagggtcgg aaacccccga tacgcaaggg ggaaggaggt cagcgcgccc ggaattcgcg
     3001 cggtgcgacg agcgatagcg caggctgcgt cacgcccgcg atcatatcga gccgcgagac
     3061 cgtgtcgggg ccgagcgcga agccgcggcc gagttcgatg cggatttcgt cgtcggtcag
     3121 cggatcgatc gtggtgacga tcacgcggcc cttcgcatcg tcgcgcttga cgagcagccg
     3181 cgccatgtcc tcgatcgccg ccggcatgtc gacgcggcag gttagctgga gtgccgcggt
     3241 cgcggccata tcggcaagcg actgcgcgcc gcgcacggtg acccgcggcg tttcctcgcc
     3301 ttcgagcaga tcgagttcga ccgagagcat cgcgcagccg ccgtcggccg ccagcgcctc
     3361 gagcgtcttt cccgcgattt cgtcgaagca gctcgactgg aactggccgc tgcggtccga
     3421 aagggtcaag ttgaggaaac ggttgccgcg ctgcgacacg cgcgggcgcg cgctttcgac
     3481 catcgccgcc atcaccatcg ggatgcgcgt tcccggcgac atttcgacat tgtcgcagat
     3541 gtcgccatag gagcgcgcgc cgcgtgccga gacgatcgcc tcatattgct cgaccgggtg
     3601 cgacgagaaa tagaagccga aggcgtcctt ctcgcgcgtc atccgctcgg cgagcgtcca
     3661 tggttcggcg gcgggcagtt gcagcgccgg ggcgatcgcg acgtcgccgc cgaacagccc
     3721 gccctgcccc gtcgagcgtt cgtgcgccga actgtcctgc ggctcctgag cccaggacgc
     3781 ttgagcgccc accaatgcag
//
LOCUS       DATMSU010000248         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_146516,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000248 DATMSU010000000
VERSION     DATMSU010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146516"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..592
                     /locus_tag="VN034_06595"
     CDS             158..592
                     /locus_tag="VN034_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HWV60302.1"
                     /translation="MSDFRPLSAQFSVAPQIDIEDVAEAKAQGFALVINNRPDGEEPA
                     APQGEHISTACAAEGLAYAAIPIGHAGFSHAQIDALDKLLASATGPILAYCRSGTRST
                     HLWALTRARAGDDVGGIVDAAAKAGYDLSGLRPMLDALSGEK"
     gene            589..996
                     /locus_tag="VN034_06600"
     CDS             589..996
                     /locus_tag="VN034_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60303.1"
                     /translation="MTQAALIQLGASLVTVLFVAWLVGKMGLGADPRIEDAAHAIRLA
                     EEAEAGFRGTEVARDRAGFAAIVRNAEGRMMLVRAHGNHFAARPVDASVIGRLDKDFL
                     TLTTPERTFGAVTLQLGKDAGMWASRMREFGRA"
     gene            989..1903
                     /locus_tag="VN034_06605"
     CDS             989..1903
                     /locus_tag="VN034_06605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWV60304.1"
                     /translation="MPELPDPVVYAVPAFILLLIVEMLISLRRDKSRYEARDTLTSLM
                     LGTVSQVAGALVGAAVIGMAVWVYQFRLFDIGIEFSTWWWAWILCFFLDDLSYYAFHR
                     SAHRVRWFWASHVIHHSSQHYNLSTALRQTWTGFFSLTFLFRLPLFLIGFPPAMVFFC
                     AGLNLIYQFWIHTEAIKRLPRWFEAVMNTPSHHRVHHGVNPRYLDANYAGVFIIWDKM
                     FGSFVPERDDEPVRYGIVKQLGSFNILWAAVHEWVGIAKDVWHAPWRHKLSYMWREPG
                     WSHDGSRATSAMIKERWAAGRPAEETAE"
     gene            2268..>3685
                     /locus_tag="VN034_06610"
     CDS             2268..>3685
                     /locus_tag="VN034_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWV60305.1"
                     /translation="MDQFDIIVIGGGSAGSAAAGRLAEDGKRTVCLVEAGGRNDNMLI
                     KTPGFMPFIRNSSNYKYETVPQKGLNGRIGYQPRGRGLGGSSAINAMVYIRGHAFDYD
                     QWASLGATGWSYADVLPWFKRAEHNERGADEYHGAGGPLNVMDQRWPNVTSRRFVESA
                     AALQLPRTGDFNGPSNEGFGLYQVTQKGGERWSAARAYVEPLREHGNFAVRTGALVEK
                     ILIENGRAAGVEIRRGAKRETLRARGGVILSAGAFNSPQILMLSGIGPAAHLRDKDIE
                     VVRDRAGVGANLQDHIDYVSSWETRSADPFGDSPGGTWRMLKAIFEHRTKRSGIMTTC
                     FAEAGGFWKSRPDLPAPDIQYHFVPAMLEDHGRTKVKGHGFSCHACVLRPESRGSVTL
                     ASGDAAAAPVIDPGFLTDARDMATLRAGVRMMHRIAAAPPLSDYGGTDRHPVDLDDDT
                     SLDALIRNRADTVYHPVGTCRMG"
BASE COUNT          622 a         1169 c         1242 g          652 t
ORIGIN      
        1 ggcgttcttt tctggctcgc tgcgctcgcg cacccacccc taacccctcc ctaaaaggga
       61 ggggaagatc gtcaatccac cccagagccg ccctccttcg gttgcgttcg caccttcacg
      121 cttgtcagca agtgcgcagc gggagtagat cggcggcatg agcgatttcc gtcccctgtc
      181 cgcccagttc agcgtcgcgc cgcagatcga catcgaggat gtcgccgaag ccaaggcgca
      241 aggctttgcg ctggtgatca acaaccgtcc cgacggcgag gagcccgcgg cgccgcaggg
      301 tgagcatata tcgaccgcct gcgccgccga ggggctggcc tatgccgcga tcccgatcgg
      361 ccatgcgggg ttcagccacg cgcagatcga tgcgctcgac aaattgctgg cgagcgcgac
      421 cggaccgatc ctcgcctatt gccgttcggg aacgcgttcg acgcatctct gggcgctgac
      481 gcgcgcgcgc gccggcgacg atgtgggcgg catcgtcgat gcggcggcga aggcgggcta
      541 tgatctttcg gggctgcggc cgatgctgga cgcgctttcg ggggaaaaat gacgcaagcc
      601 gcgttgatcc agcttggcgc gtcgctcgta accgtcctgt tcgtcgcgtg gctggtcggc
      661 aagatggggc tcggcgccga tccgcgcatc gaggatgccg cccacgccat ccgcctcgcc
      721 gaagaggccg aagcggggtt ccgcgggacc gaagtggcgc gcgaccgcgc gggctttgcg
      781 gcgatcgtcc gcaatgccga gggtcggatg atgctggtgc gcgcgcacgg caatcatttc
      841 gcggcgcggc cggtcgacgc gagcgtcatc gggcggctcg acaaggattt cctgacactg
      901 acgacgccgg agcggacatt cggtgcggtg acgctgcaac tggggaagga tgcggggatg
      961 tgggcatcgc gaatgcggga gttcggccgt gcctgagctt cccgatcccg tcgtctacgc
     1021 ggttcccgcc ttcattctgc tgctgatcgt ggaaatgctg atctcgctgc gccgcgacaa
     1081 gagccgctac gaggcgcgcg acacgctgac ctcgctgatg ctcgggacgg tgagtcaggt
     1141 cgccggcgcg ctcgtcggcg cggcggtgat cggcatggcg gtgtgggtct atcagtttcg
     1201 tctgttcgac atcgggatcg aattcagcac ctggtggtgg gcttggattc tctgcttctt
     1261 cctcgacgac ctctcctatt acgcctttca ccgcagcgcg caccgcgtgc gctggttctg
     1321 ggcgagccat gtcatccatc acagcagcca gcattataat ctatcgaccg cgctacggca
     1381 gacatggacg ggctttttca gcctgacctt cctcttccgc ctgccgctgt tcctgatcgg
     1441 ctttccgccc gcgatggtct tcttctgcgc ggggctgaac ctgatctacc agttctggat
     1501 ccataccgag gcgatcaagc ggctgccccg atggttcgag gcggtgatga acacgccgtc
     1561 gcaccaccgc gtccaccacg gcgtcaatcc gcgctatctc gatgcgaact atgcgggcgt
     1621 cttcatcatc tgggacaaga tgttcggcag cttcgtcccc gaacgcgacg acgagccggt
     1681 gcgctacggc atcgtcaaac agctcggcag cttcaacatc ctctgggccg cggtccacga
     1741 atgggtcggc atcgccaagg acgtctggca cgcgccgtgg cggcacaagc tctcctatat
     1801 gtggcgcgag cccggctgga gccacgacgg cagccgcgcg acgagcgcga tgatcaagga
     1861 acggtgggcg gcggggcggc cggcggagga gacggcggaa tagccgttcc agcggagcgt
     1921 gaataaatag ggactgtccc tattctttcg cgcagaggcg cagagatcgc agagaaggta
     1981 gggaaatagt tcacgcggag acgcggaggc gcggagaaac atcccgtctc cgttcgtgct
     2041 gagcttgtcg aagcaccgct tctctttcac cgccgcaagg aagaacggcc cttcgacaag
     2101 ctcagggcga acggctttct gtttttctcc gcgtctccga gtgaacctca tcttcctctg
     2161 cgcgctctgc gactctgcgc gaaatcctct tcttccttgc ctccccacct aatgacattc
     2221 atgtaagtcg taatggaccg ccgacagggc ggcgagggga gcggtacatg gatcagttcg
     2281 acatcatcgt catcggcggc ggcagcgcgg ggagcgcggc ggcggggcgg ctcgccgagg
     2341 acggcaagcg caccgtctgt ctggtcgaag cgggtgggcg caacgacaat atgctgatca
     2401 agacgccggg cttcatgccc ttcatccgga acagctcgaa ttataaatat gagacggtgc
     2461 cgcaaaaagg gctgaacggc cggatcggct atcagccgcg cggccgcggg ctcggcgggt
     2521 cgagcgcgat caatgcgatg gtctatattc gcggccacgc cttcgattac gaccagtggg
     2581 catcgctcgg ggcgacgggc tggagctatg ccgacgtcct gccctggttc aaacgcgccg
     2641 agcataatga gcgcggcgcc gacgaatatc acggcgcggg tgggcccctc aatgtcatgg
     2701 accagcgctg gcccaatgtg acgagccggc gcttcgtcga gagcgcggcg gcgctgcaac
     2761 tgccgcgcac cggcgatttc aacggaccga gcaacgaagg gttcggcctt tatcaggtga
     2821 cgcagaaggg cggcgaacgc tggtcggcgg cgcgcgccta tgtcgaaccc ttgcgcgagc
     2881 acggcaattt cgcggtgcgc accggcgcgc tggtcgagaa gatcctgatc gagaacggcc
     2941 gcgcggcagg ggtcgagatc cggcgcgggg cgaaacgcga gacgttgcgc gcgcgcggcg
     3001 gggtaatcct gtcggcgggc gccttcaaca gcccgcagat tttgatgctg tcggggatcg
     3061 ggcccgcagc gcaccttcgc gacaaggaca ttgaggtcgt gcgcgaccgc gccggcgtcg
     3121 gcgcgaacct gcaggatcat atcgactatg tgtcgagttg ggagacgcgc tcggccgatc
     3181 cgttcggcga cagtccgggc ggcacctggc ggatgctgaa ggcgatcttc gagcatcgca
     3241 ccaaacgcag cgggatcatg acgacctgtt tcgccgaggc gggcggcttc tggaaatcgc
     3301 ggcccgacct gcccgcgccc gacatccaat atcatttcgt ccccgcgatg ctcgaggatc
     3361 atggccgcac caaggtcaag gggcacggct tttcgtgtca cgcctgcgtg ctccggcccg
     3421 agagccgcgg atcggtgacg ctggcgtcgg gcgatgccgc cgcggcgccg gtgatcgatc
     3481 ccggcttcct gaccgacgcg cgcgacatgg cgacattaag ggcgggggtg cggatgatgc
     3541 accgcatcgc cgcggcgccg ccgctgtccg attatggcgg gaccgaccgg catccggtgg
     3601 atctggacga cgatacctcg ctcgacgcgc tgatccgcaa tcgcgccgac accgtctatc
     3661 accccgtagg cacgtgccgg atggg
//
LOCUS       DATMSU010000249         6977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_99283, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000249 DATMSU010000000
VERSION     DATMSU010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99283"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VN034_06615"
     CDS             complement(<1..791)
                     /locus_tag="VN034_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HWV60306.1"
                     /translation="MRIQSLSAAVISGWPRWMPAAGLVALLPHPEQTNSIALAGTAAI
                     LVGCILAAVHHGEVVAHRVGEPFGTLILAAAVTIIEASLILSMMLSGSANPALPRDTV
                     FAAIMLILNGVVGGCLLVGGIRHGEQRFVLEGVNAALCVLAAMATLVLVLPSVSANAV
                     APHYSAAQLVFTAIVSTLLYATFVFVQTIRHRDYFLPEGAGALAGDAHAAPPSARAAA
                     ASLGLLLLALVSVVLLAKSLSQPLEAAIAAAALPRALVGIAVAARS"
     gene            complement(908..1276)
                     /locus_tag="VN034_06620"
     CDS             complement(908..1276)
                     /locus_tag="VN034_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60307.1"
                     /translation="MTLFLSVILIAPSVSEASEAPTQACFEIFADSEGEVARSSDPAW
                     REKARPKIEKLRAESERRRSRVDSELAKLRRDRDRLQRDYDRGAIHDDDFKSARFELD
                     ADVRTLVAERDAKQIPACGF"
     gene            complement(1895..2554)
                     /locus_tag="VN034_06625"
     CDS             complement(1895..2554)
                     /locus_tag="VN034_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60308.1"
                     /translation="MKAALPISWTSGVLSSVRRLRASNLGDPFRRVCSLALLSLTAAC
                     GPQAITNGDRLVAGSLLPSKSALIRETIEISRGYGGEGYGEHLLSFALSSNDVLTVTH
                     AFRPDDKIVRREVFTLSPRVAANVREQLWRIRPAKLEGYGADVRPLGCKKSFDHDFGE
                     ITVLFYPDEGTFGFFTLPYPDSCNNAAAQKARATLEEVLAMFPRSKVAESFLVAKKAS
                     Q"
     gene            2897..3376
                     /locus_tag="VN034_06630"
     CDS             2897..3376
                     /locus_tag="VN034_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60309.1"
                     /translation="MEAPTKSEEGVLNLSLANMRATNGSCLVSTVSPATTVLWPDALL
                     FKGDSKLFRAGDARFDEIAAAWARAKPKRSATFQIPSQALQHGFRIIDAASSVPGCQQ
                     RLALRSPVFDSDFAFVITDVETTGIAGGDFRISIFQRKGDQWRSVAFGSARWGHPII"
     gene            complement(3501..4106)
                     /locus_tag="VN034_06635"
     CDS             complement(3501..4106)
                     /locus_tag="VN034_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV60310.1"
                     /translation="MTGLATARESRQKISREDMRDRIIDVARSLFEEKGVEAMSMRAI
                     ASRVGIPTMTLYGYFPSKTAIIRGLWSFAFDPMFAEMRRAEDAIADPVERLVRVAHIY
                     VDYWIQHPERYRMVFMVEDRREGDDPNWFVDETDVTQSYRRFGPMIAAARGTPEIDCK
                     VDAEALICALTGVAHMEITVSEYPWAKPANYVDAILTAFTR"
     gene            4211..6508
                     /locus_tag="VN034_06640"
     CDS             4211..6508
                     /locus_tag="VN034_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60311.1"
                     /translation="MRRSRWVVLGSVGLAAVATPSLAAAQSVADEASAADGGQVEDIV
                     VTAQRRPERLQDVPVSVTALSGDAMARQRILSAGDLAKVVPNLQSVSTVGNNTPIFAL
                     RGISMSDYSANQASPVATYYDEVYKGNFALLGVALYDLERVEVLRGPQGTLYGKNTTG
                     GAVNLISAKPQFAPSGALSAGYGNYDRIESSGFVNAPLGETVAARLAFTFARADGTLK
                     NLSPGQGDLDAVREYGARLSLRFEPSSDAEFILRLSTSLQNPNNYGIIAEPGPFGIGG
                     GVYASLNAADPALNPRLDDFRTGVGRRELTSDFTPKRRNRTHAAALTSSIRLNDALQL
                     VSVSSWDRGTLLSEEDTDGSALKALSILYRDRASQVTQDIRLVGDGDGPLKYTVGGFF
                     GYEKVFNGTELRTYQDIDFNLDGALDFNDCLTAGPFAGCRLANSFYQKRWSWAAYADA
                     SYDLGGGFKLRGGLRYSRDRGRQYDVKSIISGSDGVPLANLIPGDPANLDATTSQRFN
                     KGNVSGKIGVDYLRGGNLLYASYSRGYRASSFAAQAFFASSELTIARPEKVDSFEAGF
                     KLDLLGRKLRLNGAGFYYRYRDQQFIDVNPNGTQALLNLPRSRIFGAEVELVARPISG
                     LSVSASLGLLDTKIIEGVVQGRSVAGNHLSNAPALNLSGAVDWDVLRSDAGTLSLHID
                     ASYVSRQEFDALNTPSLSQGGYGLLGARIAFRTADDRFGIALWGKNLTDHYYHTSRIA
                     IGSVGFNYTHVGTPRTYGVTLDAKF"
     gene            6521..>6977
                     /locus_tag="VN034_06645"
     CDS             6521..>6977
                     /locus_tag="VN034_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV60312.1"
                     /translation="MSSNQNGLSRREAIVMAASGVAVAGMPGSASAKDVAQTVDVIIV
                     GAGFAGLTAARELRRAGQSVVVLEADDRVGGRTKAATLAGETIDIGGQWVGPTQTHLL
                     ALAKDYGVRAVPQYADGENIIDIAGRMARYRGETPGLADADLAEFGAAVG"
BASE COUNT         1381 a         2061 c         2180 g         1355 t
ORIGIN      
        1 gatctcgcgg cgacggcaat gccgacaagc gcgcgcggca acgccgcagc cgcgatcgct
       61 gcttcgagcg gttgcgagag gcttttggcg aggaggacca cgctgaccag cgcaagcaga
      121 aggaggccga gcgaagccgc cgcagcgcgc gcgctcggcg gcgcggcatg cgcatcgccg
      181 gcgagtgcac ctgcgccctc gggcaggaaa tagtcgcgat ggcggatggt ctggacaaat
      241 acgaaggttg cgtagagtag cgtcgacacg atcgcggtaa acacgagctg cgcagcggaa
      301 tagtggggag cgacggcgtt cgcgcttaca cttggcagaa ccaagaccag cgttgccatc
      361 gccgccagca cgcagagcgc ggcattgacg ccctcgagca cgaagcgctg ctcgccatgg
      421 cgaataccgc cgaccagcag gcagccgccc acgaccccgt tgagaatgag catgattgct
      481 gcaaacaccg tatcgcgcgg caaggccgga ttcgccgacc cgctcagcat catcgacaaa
      541 atcagcgagg cctcgataat tgttaccgcg gcagcgagaa tcagcgtccc gaacggctcg
      601 cctacccggt gggcaacgac ctcgccatga tggactgcgg cgaggatgca gcccacaagg
      661 attgcggcgg ttcccgcaag agcgatgctg tttgtttgct ccgggtgcgg caaaagcgcc
      721 accaggcctg cggcaggcat ccaccgaggc cagcccgata tcaccgcagc cgaaagggac
      781 tggattcgca tgacgcgcct ttctgaaaca ggccggcgcg cgaaagtcaa acgtgcaact
      841 cagttttcct cgatcagctg agttcgacgg aagccgggac ggatttgagt cctttagctg
      901 gtgccgatca gaagccgcag gccggtatct gcttcgcgtc gcgctcggcg acgagggttc
      961 gcacgtcggc atcgagctcg aagcgcgccg atttgaagtc gtcgtcgtga atcgcacccc
     1021 ggtcgtaatc gcgctgcagc cggtcgcgat ctctcctaag ttttgcgagt tcgctgtcga
     1081 cccgggaccg gcgccgttcg ctctcggcgc gcagcttctc gattttgggc cgagccttct
     1141 cccgccaggc gggatcgctc gaacgggcca cctcgccctc ggaatcggcg aaaatctcga
     1201 agcaggcctg ggtaggtgct tcggatgctt cggaaacgga cggcgcgatc aggattacgg
     1261 agaggaaaag cgtcatggtc gttcgatagc gaacgtcatt cccggtttct accggggcat
     1321 acagcgagcg caaaagccca cagttcgcgt tcaagcatca tcatcggaac agattttgcg
     1381 gcgcagacac gatggaggac agccgagcga taaaagcccc cgccaggcgt tagccgagcg
     1441 gggtcagttg ggggtgccct ttcgtcacag gagggcggcg gaagaacgct tttcgcccga
     1501 acagggttgc acgaaacggc tcatgaaagc gagaagggcc ccgccggcgg gcgaagccct
     1561 tccatcctct cgtcatggct acgcaagagg agccgctagc ccaacggaga cgccaatcaa
     1621 tgggctttca tggcgccgga attttatgaa aagacgatcg atagtcaaga ttgtggctgg
     1681 caggcgggaa cctcagcgag tgtgcctcgt ttcggtgccg cgatacggtg gacatggggc
     1741 gagggccgaa agatataaga gacggtcgcg ctcaatgtcg gcttttctcg gcttcgttcc
     1801 taaaaccgga ccggctgcta tcggccaggt caagcggtcg aactcaccgg ttatgcccag
     1861 ttcgctgtca tggccggaac cgggtgggat gcgattattg acttgccttc ttagcaacga
     1921 gaaacgactc tgcgaccttc gatcttggaa acattgccaa aacctcctcg agtgtcgctc
     1981 tcgccttctg cgctgcagcg ttgttgcagc tgtctgggta aggcaaggtg aagaacccga
     2041 acgtcccttc gtccggatag aagagtaccg tgatttcccc gaaatcgtga tcaaagctct
     2101 tcttgcatcc taacggacgg acgtcggcgc cgtagccttc tagctttgca ggacgaatgc
     2161 gccacaattg ctcccttacg ttcgcagcaa cgcgcggcga taacgtaaag acttcccttc
     2221 taacaatttt atcgtccggt ctgaaagcgt gcgtgacggt cagaacgtcg ttggaggaaa
     2281 gcgcgaaaga gaggagatgc tcgccgtatc cttcaccgcc atagcctctg ctgatctcga
     2341 tcgtctcgcg gataagagcg gatttggagg ggagcaaact accagctacc aatcgatctc
     2401 cattcgttat tgcctgagga ccacaggcgg ccgtcaatga cagaagcgca aggctgcaaa
     2461 cacggcgaaa cggatcacct aagttagaag cgcgtaatcg cctcacagaa gaaaggacgc
     2521 ctgacgtcca agagataggc aaagcggctt tcatgagaca ttgttacatc actctgcgaa
     2581 tgtctgcaat tggtcgcttt acatcgttca cacgcgatcc tggcgaatgt cggatatcta
     2641 ggtttggcgg ccggaagctg ccggtccgca atcggccagg acaagcggtc gaactcgccg
     2701 gctactccca actcgccgtc gtggccggaa cggagtggca agcggacatt acttcggcgt
     2761 agaacatagt tgcagctttt gtcgcaagat atgttattac atggacatgt caggtcgacc
     2821 tttccgaaca gcgatgctca ctttgccact cttcctgtgc gcatgcgaag gtcgacggga
     2881 tgctcaagag aaggtgatgg aagcaccgac taaaagcgag gaaggcgtct tgaacctctc
     2941 cctcgcaaac atgcgggcga cgaatggttc atgtcttgtt tctactgtaa gcccggcgac
     3001 gaccgtgctt tggcccgatg cgctattgtt caaaggtgat agcaagctgt tccgcgcggg
     3061 cgatgcgcgg tttgacgaaa tagccgcagc atgggcgcga gcgaaaccca aacgctccgc
     3121 tacattccag atacccagcc aagccttgca acatggattc cgcattatcg acgccgcatc
     3181 atcggtgcct ggatgtcagc aacgattggc cctgcggtcg cccgtttttg atagcgactt
     3241 tgcgtttgta atcactgatg ttgagaccac aggcatcgct ggaggcgatt ttcgcatcag
     3301 catatttcag cgcaagggag atcaatggcg aagcgtcgcg tttggaagcg ctcgctgggg
     3361 gcatccgatc atctgacaat ggcaacgatc tgacgtcagc aatcggtcga atgctgtcat
     3421 ctccgcccca tcgggttcga atgtccgctt ctctaggtcc cccacctaga agctgccagt
     3481 ccgaaatcgg ccagccgcgg tcagcgcgta aaggcggtga gtatcgcatc gacataattg
     3541 gcgggcttcg cccaagggta ctcgctgacg gtgatttcca tatgggcgac gccggtgagc
     3601 gcgcaaatca acgcttccgc atcgaccttg caatcgatct ccggtgtccc gcgcgcggcg
     3661 gcgatcatcg gtccgaaccg cctgtaggat tgtgtgacat cggtttcgtc gacgaaccag
     3721 ttggggtcgt cgccttcgcg gcggtcctcg accatgaaca ccatgcgata gcgctcgggg
     3781 tgttgtatcc aatagtcgac atagatgtgt gcgacgcgga ccagtcgttc gaccggatcg
     3841 gcgattgcat cctccgcacg ccgcatctct gcgaacatgg gatcgaatgc gaatgaccat
     3901 agcccgcgga tgatagcggt cttgctcgga aagtagccat agagcgtcat ggtcggaatt
     3961 ccgacacgac tcgcgatcgc gcgcatgctc attgcctcga cgcccttctc ctcgaagagt
     4021 gacctggcga cgtcgatgat ccggtcgcgc atgtcctcgc gcgagatttt ctggcgggat
     4081 tcccgcgcgg tggccagtcc ggtcaaaata gctccttgac gtttccgtac gcgtacgaat
     4141 aaacgtttcg tacgcgtacg gaagagccta gcgcatcagg ccgccggcgt cgatacctgg
     4201 ggaggtagat atgcgtcgat cgagatgggt ggttttgggt tcggtgggct tggcggcggt
     4261 cgccacgccg tcactcgcgg cggcgcagag tgtcgcggac gaagcctcgg cagcggatgg
     4321 cggtcaggtc gaggatatcg tcgtaacggc gcagcgccgc cccgaacgat tgcaggatgt
     4381 gcccgtgtcg gtcacggcgc tgtcgggtga tgcgatggcg agacagcgga ttctcagcgc
     4441 cggcgatctc gcaaaggtcg tcccgaacct tcaatcggtc agcaccgtcg gcaacaacac
     4501 gccgatcttc gcactccgcg gcatctcgat gtccgattac agcgcaaatc aggcgagccc
     4561 ggtcgcgacc tattatgacg aggtttacaa gggtaatttc gcgctgctgg gcgttgcgct
     4621 ttacgatctt gagcgggtcg aggtgctgcg tgggccgcag ggcacgctct atggcaagaa
     4681 taccaccggt ggcgcggtca atctgatttc ggccaagcct cagttcgcgc cttcgggcgc
     4741 cctgtcggcg ggttacggaa actacgatcg catcgaaagc tcgggcttcg tcaatgcgcc
     4801 gctcggcgag acagttgccg cgcggctcgc cttcaccttt gcgcgcgcgg acgggacgtt
     4861 gaagaatctg tcgccggggc agggcgatct cgacgcggtg cgcgaatatg gggcgcggct
     4921 ctcgctacgg ttcgaaccct cgagcgatgc cgagttcatc ctgcggctct cgacgagcct
     4981 ccagaatccc aacaattacg ggattatcgc cgagccgggg ccgttcggca tcggcggcgg
     5041 ggtctatgct tcgctcaacg ccgccgatcc ggcgctcaac ccgcgcttgg acgatttccg
     5101 cacaggcgtg gggcggcgcg aactcacgag cgattttacc ccgaagcgcc gcaaccgcac
     5161 ccatgccgca gcgctcacat cgagcatccg gctgaacgat gcgctccagc tcgtgtcggt
     5221 aagctcctgg gatcgtggta cgctgctcag cgaggaagat accgacgggt cggcgctgaa
     5281 ggcgctctcg atcttatacc gcgaccgtgc ttcccaagtt acgcaggata tccgcctcgt
     5341 cggtgatggg gacggaccgc tcaaatatac cgtcggcggt ttcttcggct atgaaaaggt
     5401 cttcaacggg accgagctca ggacctatca ggatatcgac ttcaatctcg acggcgcgct
     5461 cgacttcaat gactgcctga ccgccggtcc gtttgcggga tgccggctcg cgaacagctt
     5521 ctatcagaag cgctggagct gggcggctta tgccgacgcc agctatgatc tcggcggcgg
     5581 gttcaagcta cgcggcggtc tgcgctacag ccgtgaccgg ggtcgtcagt atgacgtcaa
     5641 atcgatcatt tcgggcagcg acggtgtgcc gctcgcaaat ctgatccccg gcgacccggc
     5701 caatctcgat gcgacgacgt cgcagcgatt taacaaaggc aatgtctcgg ggaagatcgg
     5761 cgtcgattat ctgcgcggcg gcaacctgct ctatgcgagc tatagccgcg gctatcgcgc
     5821 tagctctttt gcagcacagg ccttttttgc gtccagcgag ttgacgatcg cgcgccccga
     5881 gaaggtcgat tccttcgaag ccggcttcaa gctcgacctg ctgggtcgca agctgcggct
     5941 gaacggtgcg ggcttctatt accgatatcg cgatcagcag ttcattgacg tcaatccgaa
     6001 cggcacgcag gcgctgctca acctgccgcg ctcgcgcatt tttggcgccg aggttgaact
     6061 ggttgcgcgg ccgatatcgg gcttgtcggt ctcggcctcg ctcggccttc tcgatactaa
     6121 gatcatcgaa ggtgtggtgc aggggcgatc ggttgcgggc aatcacctgt ccaatgcccc
     6181 ggcgctcaat ctgtcggggg cggtagattg ggatgtcctt cgatccgatg cggggacact
     6241 cagtctccac atcgacgcca gctatgtaag tcggcaggag tttgacgcgc tgaacacgcc
     6301 gagcctgtcg cagggcggct atggcctgct cggtgcgcgg atcgcctttc gcaccgccga
     6361 tgaccgcttc ggtatcgccc tatggggcaa gaatctgacc gatcactact atcacacgtc
     6421 gcggatcgcg atcggatcgg tcggtttcaa ctacacccat gtcggcacgc cccgtaccta
     6481 cggcgtgacg ctcgatgcaa aattctgagg agaggcgagc atgagcagca accagaacgg
     6541 gctttcgcgg cgtgaagcca tcgtgatggc ggcaagcggt gtcgccgtgg ccgggatgcc
     6601 ggggagcgca tcggcgaagg acgtggcgca gaccgtcgac gttatcatcg tgggcgcagg
     6661 atttgccggt ctaaccgctg cgcgcgaact gcgccgcgcc ggccagtcgg tcgtcgtcct
     6721 cgaagccgac gatcgcgtcg gtgggcgcac caaggcggcg acactggcag gtgagacgat
     6781 cgacatcggc ggccagtggg tcggcccgac gcaaacgcat ctccttgctc tggcgaagga
     6841 ttatggcgtc cgggctgtcc cgcaatatgc cgacggcgag aatatcatcg atattgcggg
     6901 ccgcatggcg cgctaccgtg gcgagacgcc gggtctggcc gacgccgatc tcgccgagtt
     6961 cggcgcggcg gtcgggg
//
LOCUS       DATMSU010000250         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_61669, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000250 DATMSU010000000
VERSION     DATMSU010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61669"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VN034_06650"
     CDS             <1..813
                     /locus_tag="VN034_06650"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV60313.1"
                     /translation="RIDPRWGQPGIWAAMRHADGPLTRHYLLAALDRRLNADDRIVGV
                     PPEQAVFTNIYLRTFKISAIVALLCAALGYPVAYLLASLPERKANPLLILVLLPFWTS
                     ALVRTAAWAVLLQTNGVVNNALQAVGLIDAPLQLIYNRLGVYIAMTHVLLPFFILPLY
                     GVMKGIPPTAMRAASSLGAPPLRAFFAVYLPQTLPGVAAGAIIVFTLALGYYVTPALV
                     GGGADQMISASIAFYTNQSLNWGMAAALSLLLLLPLLLMVFAGRTMVRTVPA"
     gene            810..1649
                     /locus_tag="VN034_06655"
     CDS             810..1649
                     /locus_tag="VN034_06655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV60314.1"
                     /translation="MSACRSRSERVGRAALIGFSVLVFAFLVLPILAVIPLSLNPTTF
                     LVFPEGEFSLRWYRNLAESPQWTHAFGNSLKIAVATTLIATPLGTLAAIGLAYMRSRA
                     KTAIVALITAPLVVPVVVVAIAFYFLFAPLGLVNGSLGLIVAHSALAVPFVVIVVHAA
                     LRGLDTELLRAAASLGAPPLTVLLRVLVPLIAPGVAAGAVFAFMTSFDETTVALFIAG
                     PEQRTLPIQMFEGVREQISPAIAAAATLLIVASTLLLGAAELLRRRGERPNAGSTSPD
                     VGA"
     gene            complement(1623..>2100)
                     /locus_tag="VN034_06660"
     CDS             complement(1623..>2100)
                     /locus_tag="VN034_06660"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HWV60315.1"
                     /translation="ITISHTGCRALNDVPRNTADDELRALADKGGVAGIYFMPFLRAR
                     GQPRAADLVRHIEHAVKVAGEDHVALGTDGGIRGFELNAEYAEGQRRFYEERKAMGIA
                     APGEAPDVFNLIPDYNEPARFLKLADHLAARGWPARRIEKLLGANFARLCADVWGR"
BASE COUNT          269 a          734 c          673 g          424 t
ORIGIN      
        1 cggatcgacc cgcggtgggg gcagccgggg atatgggccg cgatgcgcca tgccgacgga
       61 ccgctcacgc gccattatct gctcgccgcg ctcgaccgcc ggctgaacgc cgacgaccgc
      121 attgtcggcg ttcctcccga acaggcggtc ttcacgaata tctatctgcg caccttcaag
      181 atcagcgcga tcgtcgcgct gctttgcgcc gcgctcggct atccagtcgc ctatctgctc
      241 gcttcgcttc ccgagcgaaa ggccaacccc ctgctgatcc tcgtgctcct gcctttctgg
      301 acctcggcgc tcgtccgcac cgcagcgtgg gcggtgctgc ttcagaccaa tggcgtcgtg
      361 aacaacgcgc tgcaggccgt cgggctgatc gatgcgccgc tccagctcat ctacaaccgg
      421 ctcggcgtct atatcgcgat gacgcatgtg ctgctgccct ttttcatcct gccgctctat
      481 ggcgtgatga agggcattcc gcccaccgcg atgcgcgcgg cgagttcgct cggcgccccg
      541 ccgcttcgcg cctttttcgc ggtttatctg ccgcagacat tgcccggtgt cgccgccggc
      601 gcgatcatcg tcttcaccct cgcgctcggc tattatgtca cccccgcgct cgtcggcggg
      661 ggcgccgacc agatgatcag cgcgtcgatc gccttctaca ccaaccagtc gctcaactgg
      721 ggcatggcgg cggcgctgag cctgttgctc ctgctgccgc tgctgctgat ggtcttcgcg
      781 gggcggacga tggtccgcac ggtgccggca tgagcgcctg ccgttcccgg tcggagcgcg
      841 tgggccgtgc ggcgctgatc ggtttttcgg tcctcgtctt cgccttcctg gtgctgccga
      901 tcctcgcggt gatcccgctg tcgcttaatc cgacgacctt cctcgtcttc cccgaaggcg
      961 aattttcgct gcgctggtac cggaatctcg ccgaatcgcc gcaatggacg catgcgttcg
     1021 gcaacagttt gaagatcgcc gtcgcgacga cattgatcgc gacgccgctc ggcacgctcg
     1081 cggcaatcgg gctcgcctat atgcgctcgc gcgccaagac ggcgatcgtc gcgctgataa
     1141 ccgcgccgct cgtcgtcccg gtcgtcgtgg tcgcgatcgc cttctatttt ctcttcgcgc
     1201 cgctcgggct cgtcaacggt tcgctcgggt tgatcgtcgc gcatagcgcg cttgcggtgc
     1261 cctttgtggt gatcgtggtc catgcggcac tgcgcgggct cgacaccgag ttgctgcgcg
     1321 ccgccgcaag ccttggcgca ccgccgctca ccgtgcttct tcgcgtcctt gttcccctga
     1381 tcgcgcccgg ggttgcggcg ggtgcggtgt ttgccttcat gacctctttc gacgaaacga
     1441 ccgtcgcgct gttcatcgcg ggtcccgaac agcgcacgct gccgatccag atgttcgaag
     1501 gggtacgcga gcagatcagc ccggcgattg ccgcggcggc cacgctgttg attgtcgcct
     1561 cgaccttgct gctgggggca gccgaactgc tgcggcgccg cggcgaacgg cccaacgccg
     1621 gatcaacgtc cccagacgtc ggcgcatagc cgcgcgaaat tggcgccgag cagcttttcg
     1681 atccgccgcg ccggccagcc gcgcgcggcg aggtggtcgg cgagcttcag gaagcgcgcg
     1741 ggctcattat aatcggggat caggttgaac acgtcgggcg cttcccccgg cgccgcgatc
     1801 cccatcgcct tgcgttcctc gtagaagcgc cgctggcctt cggcatattc ggcgttgagt
     1861 tcgaagccgc ggatgccgcc gtcggtgccc agcgcgacat gatcctcgcc cgcgaccttc
     1921 accgcatgtt cgatatggcg gaccaggtcg gcggcgcgcg gttggccccg cgcgcgcagg
     1981 aagggcatga aatagatgcc ggcgacgccg cccttgtcgg cgagcgcgcg gagttcgtcg
     2041 tcggccgtgt tgcgcggcac gtcgttcaag gcgcggcagc cggtgtggct gatcgtgatc
//
LOCUS       DATMSU010000251         6951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_137048,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000251 DATMSU010000000
VERSION     DATMSU010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137048"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1596
                     /locus_tag="VN034_06665"
     CDS             148..1596
                     /locus_tag="VN034_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HWV60316.1"
                     /translation="MLFTPTLSADRDLVIAPDYAAWGKAAREHDRKSGLQAWRDADES
                     KHFDYKAIRARLEKLRKLSAAGDVKGLLFVLNEGIHGNIDGMGHERLYQKARFGTKTL
                     VEDYVAEVVASLDKIAAARSIAREEKRDFFRRAQHCYGRSALLLSGSGSFLFFHIGVV
                     KALWEEGVLPAIMSGASGGSIVAAIVCTRKNADIGAFFESERLANPDRDPEGRRLAPD
                     EVRERLAELIPDLTFQEAYEVSGRHLNVSVAPAEKHQNGRLLNAITAPNVLIREAVLA
                     SCAVPGVFPPVMLMARDDDGNRIPYQPDRRWVDGSVTHDIPTKRLERLYGVNHHIVSQ
                     ANPIALPFATDTRKQMAPIEAIQHASMTTFKAWLNANMVIFQKPLELIPPLNSIANMA
                     RSVINQEYTGDINIIRPPKFWSPAKILSDLGQEDIDELIDTGERTAWPKVEMVRTQTA
                     ISRALEAILAKIDRGGDDGPGHRSAALKKAVR"
     gene            1599..2447
                     /locus_tag="VN034_06670"
     CDS             1599..2447
                     /locus_tag="VN034_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HWV60317.1"
                     /translation="MASGAVILPAESAGAGAKLHVTHWLPEGRPRAVVLLAHGYAEHA
                     GRYAHVAKRLTDAGYAIYAVDHWGHGASDGEGGYVPRFSAFLDGMAELLTLVEINHGD
                     TPRLLLGHSMGGLIATLFLVERQQAFAAAALSGPAILPAEPPSRVTIWISRFLSRFFP
                     RLGVLSLDANGVSRDPAVVEAYRADPLVYEGKIGARLGKEFMDAMAVAQADAPKIRLP
                     ILIQHGEADRLTAPAGSRYLFDNVASRDKRLEIYPGLFHEIYNEPEQGAVLDDLVGWF
                     DAHVAN"
     gene            2601..3533
                     /locus_tag="VN034_06675"
     CDS             2601..3533
                     /locus_tag="VN034_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV60318.1"
                     /translation="MKILLVLIFAPLITLALLYYLFPEQLVRLMRWLMRRRARVVRKS
                     VTVDGRRWPYLEGGDPSKPTLVMVHGFGADKDHWTFYAPWMTRDYHLVAPDLPGFGEN
                     DRDGELPFDVASQAARLKAFLDALGIERPHLGGNSMGGWIALRFAIDYPGALRTLTLM
                     NNAGIMGAEESELQRLAADRDYNPLVIANLEDADRLIAFVVHKPTFVPARLKPVIYAD
                     ALRHRDLLDQIFWIIADEMEQKPLNDELGKVKVPTLILWGRHDKLIDVSCVAVLEAGI
                     AGSRAHIFDHVAHVPMIEDPKATAEVQRAFLANH"
     gene            3574..4575
                     /locus_tag="VN034_06680"
     CDS             3574..4575
                     /locus_tag="VN034_06680"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWV60319.1"
                     /translation="MRLKVGLLGGGSWGTTVAAVVSRNAPITLWARDAETVESINGAG
                     ENRKYLPGIKLPTALRATNDMAEVVAGADVLVMGVPSHSFRAVLEEARHHLRPWVPVI
                     SLTKGLELASGKRMTELIEEVLPGHPVGVLTGPNLAREIMTGQAAASVLSMEDEIVVR
                     ALQPVFHSGLFRVYTNTDLLGCELGGVLKNIIAIAVGMGDGLGAGDNTRAGLMTRGLA
                     EITRLGVAMGGRPETFAGLTGMGDLIATCTSPLSRNRHVGVELGKGRPIDEIIAGMNM
                     VAEGVKSAPTVMALADQHGLDMPIARDVFDVTQGRRSALDVFRGLLKSSVGDEAHPG"
     gene            4695..5033
                     /locus_tag="VN034_06685"
     CDS             4695..5033
                     /locus_tag="VN034_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60320.1"
                     /translation="MKKWTTLAMLAALPATAAVAAVPYHAMPPGFEAPQIRAEPIAGI
                     VNQYWYNYQADILEAEKELSSDLRHATDREDRWDAWDEWIVEVVDADKDYVKEMRKKG
                     YRSGRVTVGG"
     gene            complement(5138..5761)
                     /locus_tag="VN034_06690"
     CDS             complement(5138..5761)
                     /locus_tag="VN034_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWV60321.1"
                     /translation="MLLLSGVFAIRRVPNPSLAILAVLLAGSIAGCDREKQAGEQAGQ
                     GQANVSAGQAKGIDAFEYVIDRSHKGTAASSAAFRGPDDAPVTLASFRGRPLLVNLWA
                     TWCAPCVAEMPTLDALAAKNGERMNVIAVAQDLKGAEVVDPWFQKARLKALQPYIDPE
                     NGLLDAANSALPTSIYYDAEGREIWRVIGAIDWQGKEAAALLAEGVS"
     gene            5760..>6951
                     /gene="argH"
                     /locus_tag="VN034_06695"
     CDS             5760..>6951
                     /gene="argH"
                     /locus_tag="VN034_06695"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWV60322.1"
                     /translation="MWGGRFGGGPAAIMQEINASIPVDKRLWEEDITASRAHAAMLGA
                     TGIIDAGDAVVIDRGLAQIAGEFAATGVPVDLALEDIHMTVESRLKELVGEAAGRLHT
                     ARSRNDQVATDFRLWTRTACERIDAGLKALQAALLARADEHADSIMPGFTHLQVAQPV
                     TLGHHLLAYVEMVARDRSRFADARARLNESPLGAAALAGTSFPLDRDATASALGFDRP
                     MANSIDAVSDRDFALEFCASASIAAIHLSRLAEEVVIWASQPFGFISLPDAWSTGSSI
                     MPQKRNPDAAELVRGRAGLLLGAFQRLAVIVKGLPLTYSKDLQDDKETVFGAFDALAL
                     SLAAMTGMVETLSFRTDRMHALAASGYSTATDLADWLVRENGLPFREAHHVVGACVKR
                     AEQLG"
BASE COUNT         1211 a         2299 c         2290 g         1151 t
ORIGIN      
        1 ccgggatccc gcttttcaac gccgccgagc gggaccccgg atcaagtccg gggtgacgag
       61 ggtgagaggc tgcgtcaaaa cagttgccaa agacaacgta agccgcaaga atcgagaaaa
      121 ataacgatgc tctcgcagga ttaccgaatg ctcttcaccc cgacgctcag cgccgaccgc
      181 gatcttgtga tcgcgcccga ttatgccgca tggggaaagg cggcccgcga gcatgaccgg
      241 aaatcggggt tgcaggcgtg gcgcgacgcc gacgaaagca agcatttcga ttataaagca
      301 atccgcgcgc ggctcgaaaa actgcgcaag ctgtcggcgg cgggggacgt caaggggctg
      361 ctcttcgtgc tcaacgaggg catccacggc aatatcgacg gcatgggaca cgagcggctc
      421 tatcagaagg cgcgcttcgg cacgaagacg ctggtcgagg attatgtcgc cgaggtcgtt
      481 gcttcgctcg acaagatcgc cgccgcgcgc agcattgccc gcgaggagaa gcgcgacttc
      541 ttccgccgcg cgcagcattg ctacggccgc tcggcgctgc tgctctcggg gtcgggaagc
      601 ttcctgttct tccatatcgg cgttgtgaag gctttgtggg aagaaggcgt gctgcccgcg
      661 atcatgtcgg gcgcgagcgg cgggtcgatc gtcgccgcga tcgtctgcac gcgcaagaat
      721 gccgacatcg gcgccttctt cgaaagcgag cggctcgcga accccgatcg cgaccccgag
      781 gggcgccgcc tcgcccccga cgaggtccgc gaacgcctcg ccgaattgat ccccgacctc
      841 acctttcagg aagcctatga ggtcagcggc cgccacctca acgtctcggt tgcccccgcc
      901 gaaaagcatc agaacggccg gctcctcaat gcgatcaccg cgcccaatgt gctgatccgc
      961 gaagcggtgc tcgcgtcgtg cgcggtgccc ggggtgtttc cgccggtgat gctgatggcg
     1021 cgcgacgacg acggcaatcg catcccctat cagcccgacc ggcgctgggt cgacgggtcg
     1081 gtgacgcacg acatcccgac caagcgattg gagcgcctct acggggtcaa ccatcatatc
     1141 gtcagccagg cgaacccgat cgcgctgccc ttcgcgaccg acacgcgcaa gcagatggcg
     1201 ccgatcgagg cgatccagca tgcgtcgatg acgaccttca aggcgtggct caacgccaac
     1261 atggtgattt tccagaagcc gctcgaactg atcccgccct tgaacagcat cgccaatatg
     1321 gcgcgctcgg tgatcaatca ggaatatacc ggcgacatca atatcattcg cccgcccaaa
     1381 ttctggtcgc cggcgaagat tttgagcgat ctggggcagg aagatatcga cgagctgatc
     1441 gacacgggcg agcgcaccgc gtggcccaag gtcgaaatgg tccgcaccca gaccgcgatc
     1501 agccgcgcgc tcgaagcgat attggccaag atcgatcgtg gcggcgacga cggccccggg
     1561 caccgcagcg ccgcgctcaa gaaagcggtg cgctgaatat ggcgagcggc gcggtgatcc
     1621 tgcccgccga atcggccggg gcgggggcaa agctgcatgt cacccactgg cttcccgaag
     1681 gacgcccgcg cgccgtggtg ctgctcgcgc acggctatgc cgaacatgcc gggcgctatg
     1741 cgcatgtcgc gaaacggctg accgacgcgg gctatgcgat ctacgccgtc gatcattggg
     1801 ggcatggcgc ttcggatggc gagggcggct atgtcccgcg cttctcggcc tttctcgacg
     1861 gcatggccga actgctgacg ttggtcgaga tcaaccatgg cgatacgccg cgcctgctcc
     1921 tcggtcacag catgggcggg ctgatcgcga ccttgttcct tgtcgagcgc cagcaggcgt
     1981 tcgccgccgc cgcgctgtcg gggcccgcga tcctgcccgc cgagccgccg tcgcgcgtca
     2041 caatctggat cagccgcttc ctgtcgcgtt tcttcccgcg cctcggggtg ctgtcattgg
     2101 atgccaatgg cgtcagccgc gatccggcgg tggtcgaggc ctatcgcgcc gacccgctcg
     2161 tctatgaagg caagatcggg gcgcgcttgg gtaaggaatt catggacgcg atggcggtcg
     2221 cgcaggccga cgcacccaag atccgcctgc cgatcctgat ccagcatggc gaggccgacc
     2281 gcctcaccgc gccggcgggg tcgcgctatc tgttcgacaa tgtcgcatcg cgcgacaagc
     2341 gcctcgaaat ctacccgggt ctgttccacg aaatctataa cgagcccgag cagggcgcgg
     2401 tgctcgacga tctggtcggc tggttcgatg cgcatgtggc gaattaagcc tgatcaaacc
     2461 cctccccttc aggggagggg cagcgagact tggtccggcg taagccgggc cctagtcgaa
     2521 cggggtgggc atcaaggccg cgctgtgctg cagaccccac cccaacccct cccctgaagg
     2581 ggaggggcta aggagacgcc atgaaaatcc tcctcgtcct gatcttcgcg ccgctcatca
     2641 cgctcgcttt gctctattat ctcttccccg aacagcttgt gcggctgatg cgctggctga
     2701 tgcggcggcg cgcgcgggtc gtgcgcaaga gtgtcacggt cgacggtcgc cgctggccct
     2761 atctcgaggg cggcgacccg tcgaagccaa ccctcgtgat ggtccacggc tttggcgccg
     2821 acaaggatca ctggaccttc tacgcgccgt ggatgacgcg cgactatcat ttggtcgccc
     2881 ccgacctgcc ggggttcggc gagaatgacc gcgacggcga actgccgttc gacgtcgcga
     2941 gccaggcggc gcggctcaag gcctttctcg atgcgctcgg gatcgagcgc ccgcatctcg
     3001 gcggcaacag catgggcggc tggatcgcgc tgcgcttcgc gatcgattat ccgggcgcgc
     3061 tgcgcacgct gacgctgatg aataatgccg ggatcatggg cgccgaggaa agcgaactcc
     3121 aaaggctcgc ggcggaccgc gactataatc cgctcgtcat cgccaatctc gaggacgccg
     3181 atcggctgat cgccttcgtc gtccataaac cgacctttgt gcccgcgcgg ctgaagcccg
     3241 tgatctatgc cgacgcgctt cggcaccgcg acctgctcga ccagattttc tggatcatcg
     3301 ccgacgagat ggagcaaaag ccgctcaacg acgaactcgg caaggtcaag gtgccgacgc
     3361 tgatcctgtg gggccgccac gacaagctga tcgacgtcag ctgcgtcgcg gtgctcgaag
     3421 cggggatcgc gggcagccgc gcgcatatct tcgaccatgt cgcgcatgtg ccgatgatcg
     3481 aggatccgaa ggcgaccgcg gaggtccagc gcgccttcct tgccaatcac tgaaactgcc
     3541 gtcactcttc ctccaaaata gcgggagagc gcgatgcggt tgaaggtggg gctgctcggc
     3601 gggggcagct ggggaacgac ggtcgcggcg gtggtgtcgc gcaatgcgcc gatcacgctc
     3661 tgggcgcgcg atgccgagac ggtcgagagc atcaacggcg cgggcgagaa ccgcaaatat
     3721 ctgcccggca tcaaactgcc gacggcgctc cgcgcgacga atgatatggc cgaagtcgtc
     3781 gcgggcgccg atgtgctcgt catgggcgtg ccctcgcaca gcttccgcgc ggtgctggag
     3841 gaagcgcgcc atcatctccg cccgtgggtg ccggtgatca gcctcacgaa aggcctcgaa
     3901 ctcgcctcgg gcaagcggat gaccgagctg atcgaggagg tgctacccgg ccaccccgtc
     3961 ggcgtgctca ccgggccgaa tctggcgcgc gagatcatga cggggcaggc ggcggcgagc
     4021 gtgctgtcga tggaggacga gatcgtcgtc cgcgcgctcc agccggtgtt tcactcgggg
     4081 ctgttccgcg tctacaccaa caccgacctc ctcggctgcg aactcggcgg ggtgctcaag
     4141 aatatcatcg cgatcgcggt cggcatgggc gacggattgg gcgcggggga caatacgcgc
     4201 gcgggcctga tgacgcgcgg gcttgcggag atcacgcggc tgggggtggc gatgggcggg
     4261 cggcccgaaa ccttcgcggg gctcaccggc atgggcgacc tgatcgcgac ctgcaccagc
     4321 cccttgtcgc gcaaccgcca cgtcggggtc gaactcggca agggacgtcc gatcgacgaa
     4381 atcatcgccg gcatgaacat ggtcgccgaa ggggtgaaga gcgcgccgac ggtgatggcg
     4441 ctcgccgacc aacatggtct cgacatgccg atcgcgcgcg acgtgttcga cgtgacgcag
     4501 ggccggcgca gcgcgctcga cgtgtttcgc gggctcctca aatcgagcgt cggcgacgag
     4561 gcgcatcccg gctgaaatcc gccggtttcg gcgattccgc cgcgagccgt gccgaatcat
     4621 cgtcgaaccg ggaataaatc gcaccgctgc ttcgtttctt ctcctgtcca acaggatcca
     4681 gaaggagacg aaaaatgaaa aaatggacga cgctggcgat gctcgccgct ctccccgcca
     4741 cggccgcggt tgccgccgtt ccctatcatg cgatgccccc gggcttcgag gcgccgcaga
     4801 tccgcgccga accgatcgcc ggcatcgtga accaatattg gtacaactac caggccgaca
     4861 tcctcgaggc ggagaaggag ctcagcagcg accttcgcca cgccaccgac cgcgaagacc
     4921 gctgggatgc gtgggacgaa tggatcgtcg aggtggtcga cgccgacaag gattatgtga
     4981 aggaaatgcg caagaaaggc tatcgcagcg gccgcgtgac ggtcggcgga taaagcctcc
     5041 ggtttcctcg agttccccga gccctgctcg ggtcgcaggg aagggcgggt gtcatggggc
     5101 acccgccctt tcttgttctg ctcattgaat cgggtagcta ggaaacgccc tccgccagca
     5161 gcgccgccgc ttccttgccc tgccagtcga tcgcgccgat cacgcgccat atctcgcggc
     5221 cttcggcgtc gtaatagatg ctcgtcggca gcgcgctgtt cgcggcgtcg agcaggccgt
     5281 tctcgggatc gatatagggc tggagcgcct tcagccgcgc tttctggaac cagggatcga
     5341 cgacctcggc gcccttcaga tcctgcgcga ccgcgatcac attcatccgc tcgccgttct
     5401 tcgccgcgag cgcgtcgagc gtcggcattt cggcgacgca cggcgcgcac caggtcgccc
     5461 acaggttgac gagcagcgga cgcccgcgaa atgaagccag cgtcaccggc gcgtcgtcgg
     5521 ggccgcgaaa cgccgcgctg gacgcagccg tccccttgtg gctgcggtcg atcacatatt
     5581 cgaacgcatc gatgcccttc gcttggcccg ccgaaacatt tgcttggcct tgccccgctt
     5641 gctcgcccgc ctgcttttcc ctatcgcagc ccgcgattga tccggccaga agcacggcga
     5701 ggatcgcaag ggacggattc gggacgcggc ggatggcgaa tactccggac agcaacagca
     5761 tgtggggcgg ccgcttcggt ggcggacccg cggcgatcat gcaagagatt aacgcatcga
     5821 tccccgtcga caagcgattg tgggaagaag atatcaccgc cagccgcgcg catgccgcga
     5881 tgctcggcgc gaccgggatc atcgacgccg gggatgccgt ggtgatcgac cgcgggcttg
     5941 cgcagatcgc gggcgagttc gcggcgaccg gcgtgccggt cgatctcgcg ctcgaagaca
     6001 tccatatgac cgtcgaatcg cggctgaagg agctggtcgg cgaagcggcg gggcggctcc
     6061 acaccgcgcg ctcgcgcaac gatcaggtcg cgaccgattt ccgcctgtgg acgcgcaccg
     6121 cgtgcgagcg catcgacgcg gggctgaagg cgcttcaggc ggcgctgctc gcgcgcgccg
     6181 acgaacatgc cgacagcatc atgccgggct tcacgcatct gcaggtcgcg cagccggtaa
     6241 cgctcggcca tcatctgctc gcctatgtcg aaatggtcgc ccgcgaccgc tcgcgtttcg
     6301 ccgatgcgcg ggcgcgtctc aacgaatcgc cgctcggcgc cgcggcgctc gcgggcacca
     6361 gcttcccgct cgatcgcgac gcgacggcat cggcgctcgg cttcgaccgc ccgatggcga
     6421 atagcatcga cgcggtctcg gaccgcgact tcgcgctcga attctgcgcc tcggcctcga
     6481 tcgccgcgat ccacctgtcg cggcttgccg aagaggtggt gatctgggcc agccagcctt
     6541 tcggtttcat cagcctgccc gatgcctggt cgacgggcag ctcgatcatg ccgcaaaagc
     6601 gcaaccccga cgccgccgaa ctcgtgcgcg ggcgcgcggg gctgctgctc ggcgccttcc
     6661 agcgcctggc ggtgatcgtg aaggggctgc cgctcactta ctcgaaggat ctgcaggacg
     6721 acaaggaaac ggtcttcggc gccttcgacg cgctcgcgct gtcgctcgcc gcgatgaccg
     6781 gcatggtcga aacgctgagc ttccgcaccg accggatgca cgcgctcgcc gcgtcgggct
     6841 attcgaccgc gaccgatctc gccgactggc tggtgcgcga aaacggcctg ccgttccgcg
     6901 aggcgcatca tgtcgtcggc gcctgcgtca agcgcgccga gcaactcggc g
//
LOCUS       DATMSU010000252         8311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_108787,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000252 DATMSU010000000
VERSION     DATMSU010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108787"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN034_06700"
     CDS             complement(<1..407)
                     /locus_tag="VN034_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60323.1"
                     /translation="MSGRPLADPACIDPDGFAAHGYPHDVWTRMRREQPIAWHDDGIR
                     EPFWALARYADITAVGKDSDRFISASGVTILRKDQMVGADAPRVPLLLTMDPPDHHKN
                     RMVLRERFKPSVMRALEEHIVARCREIADGVAAA"
     gene            475..1059
                     /locus_tag="VN034_06705"
     CDS             475..1059
                     /locus_tag="VN034_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWV60324.1"
                     /translation="MTKDEETTGPARRSRMAPADRRALILQTAAAMFRDHGYAGASID
                     AIAAASGISGPAIYRYFAGKAELLLALLEAAVAETVAAMNAVPDRPAQTADDLADILA
                     ERALSEGAIIALLHGTAGTMEPEERLRLDNLRRDAVGRLTDALRALRPELAASEAEAR
                     IVAALALVGHIERFLATGAPAQSFQRILRSILRA"
     gene            complement(1099..3504)
                     /locus_tag="VN034_06710"
     CDS             complement(1099..3504)
                     /locus_tag="VN034_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60325.1"
                     /translation="MRKQHVLLCGWAAFSAFATPAWAQDGGTAPAEEGAAPTGQAGIA
                     DIVVTARRREEAIQDIPVAVQAFSGEALESQRISNAVDLSKIVPALTSSQSSRDEENY
                     VIRGQSGSGASISGQQVTVPAYFAQVPLPIGDGGGPGYYYDLQNVQVLKGPQGTLFGR
                     NSTGGAILFEPRRPDDEFGGYIAAEYGNYDNRGLEGALNVPLGSTLRVRVAGKGLKRD
                     GFTYNATNKVWQDDRSFFSGRASILWEPSSTFSNLLVADMFRSTTNGSSNILAAVNSN
                     ALIPTLFGGAVEAALDKQNAAGPRVTFSDTPGINRKRSWGVSNITTWQVSDALTVKNI
                     FGYRRFKQLNRFDYDGSGLVILNFDTCATAASCHPRDADAPWSLNIRQVTEELQLQGQ
                     LLDDRLKFIIGLFGADLDTPDANYGHQTSVFGSVTDANQYIDDSSRALFGNVSYAFGG
                     GLEGLTATAGYRWTRDRRALTLYQVTSGRCVTGATGTVVPDPAQADACGASFVAKADS
                     DAYTIGLDYKITQQTMIYVAHRQSYRAGGTNPLAAPVLNANPPIPDALSLFRYQPEIL
                     RDVEVGLKSDFRVGDWSIRTNVAGYYQWLDDAQLNQTFAVGTQTVSALVNAASARIKG
                     VEAELTVAPTSALNLMLAYAYTDAQYGEYLDYSRRDSATNAPTLQDGRIFPFTPKHKL
                     NVSANWTLPVPASLGEVLLSANWAHKSSVTLGLVPFITQPDGSNLYDGESIEGPTDTI
                     DLTLDWKEAFGGPVDVALYVTNLTNTTYKVGGASLINSGLGINQRIYNEPRMYGVSVR
                     YSF"
     gene            complement(3565..4326)
                     /locus_tag="VN034_06715"
     CDS             complement(3565..4326)
                     /locus_tag="VN034_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60326.1"
                     /translation="MNRFAGRVALVTGAGRGIGAATAAELAKGRAALALNDVDGDRLE
                     EMAASLRKHGVRVTTHLGSVMEEDFIHAFVDEATAAHGHVDLLANNAGGGPPMTPWGE
                     FASSEFAHFRAIFEMNFFTQAMLLHALLPGMIARGYGKVVCVSSISAVLGQESGSAYA
                     SGKLALHALVSSVSKEVARHGVNVNAVVLGNPPHPSRTPDRQAYLDKLSHFDRVGRLD
                     EFGKAIAFLLSDDASYISGAAVPVDGGLIAPRLNE"
     gene            complement(4323..4904)
                     /locus_tag="VN034_06720"
     CDS             complement(4323..4904)
                     /locus_tag="VN034_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV60327.1"
                     /translation="MLYDAYACWRDLTPAIERERTTIEDKVQFLSDKMRIQEMINEYA
                     FACDSRGWDILERLYDENIERVMRGTLDETVQGRDQLIGLHAKPALPRREGVVAPPRD
                     LSKIQGLEIRHLIGTQVVRVADDNQKAWALCHYQMALVGQEDGDWHHGLHEGTYLFTF
                     SKATGEWRFTRHQIWTNNAANPMFSDPRARKAA"
     gene            complement(4928..5671)
                     /locus_tag="VN034_06725"
     CDS             complement(4928..5671)
                     /locus_tag="VN034_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60328.1"
                     /translation="MQLAGKVAIVTGAAAGIGQATARLFVERGARVVATDVDEAGLAD
                     TRQGLEPDMIETLVSDATSADDAAAAVALASDRFGGLHLLVNVVGGSRPGKTVVDFDE
                     EEWDFWVRLNLTSTFLMCRAAIPAIDAAGGGSIVNIASGAGVTGMGRNPAYVAAKGGV
                     IALTRSLAIDHAPAIRANCIAPGPILTPLMKRNRSEPEIAFMSKLSLAGRLGKPEEIG
                     ATAAFLCSDDAAFINGELINVAGGRGGPI"
     gene            complement(5817..6911)
                     /locus_tag="VN034_06730"
     CDS             complement(5817..6911)
                     /locus_tag="VN034_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60329.1"
                     /translation="MFAALGPVLPSIADHFGGNTAGAFAAQMILTMPAIGVIFGGIAG
                     GLSVERWGARPVLIAALLSYGLLGISGYFIESLGLLLVSRFFLGFFVAQVGTAIGVIV
                     GSWFEGVARAKLLGYQSAVAGFFAVSGLLLSGLLAESGGWRAPFLLYAFAFLVLFLVL
                     AGLPRGVARTARAGERPSLRPLVKLWPIYGLAMFLFVGYFMTSIQLSFLLAEDGVDSP
                     LTRSMVIATGVLAGGVFGGLNGRFYARLGERGTQIFLTVMMATGLAVIGFSTDVRMLA
                     FGALLAGGGGGMIPPHISGILLNRVPADLRARAIGLEFTVLYIADFVNPLIMTPLRAM
                     MGIHNAFLIAAAALAAAALAMGWFKNRVAQ"
     gene            complement(7279..7698)
                     /locus_tag="VN034_06735"
     CDS             complement(7279..7698)
                     /locus_tag="VN034_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWV60330.1"
                     /translation="MLGGCGLDGITPHVLRHSFANIANDLGFTETTVAALLGHAKGSV
                     TDRYIPTMDAALIAAADTVAGYVPGLLDGAHYKLTAYAVDHASRKHALAIAAGEIRPN
                     SAAWDARWETFLCPFQICPSPARSPCSENKMTWERIA"
     gene            complement(7714..>8311)
                     /locus_tag="VN034_06740"
     CDS             complement(7714..>8311)
                     /locus_tag="VN034_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWV60331.1"
                     /translation="QIAAAGGNFSQSAAEARAGILADLARAESDARLRKEELTKAAKR
                     SSLQRLVSPVDGTVTQLAVHTVGGVVEAAKPIMVIVPSRSKLVAEVAIANKDVGFVGA
                     GQPVALKVEAFPFTRYGAVPGKLKHISSDAVQDEQRGLIYTARVTLDRATIVRDGRAI
                     PLTPGMAVTADIRTGRRSLASYLLSPIDEIRATAARER"
BASE COUNT         1515 a         2660 c         2633 g         1503 t
ORIGIN      
        1 gctgcggcga cgccgtcggc gatctcgcgg cagcgcgcga cgatatgctc ctcgagcgcg
       61 cgcatgacgc tcggcttgaa ccgttcgcga aggaccatgc ggttcttgtg atgatcgggc
      121 ggatccattg tcagcagcaa cggcacgcgc ggcgcatcgg cgccgaccat ctggtccttg
      181 cgcaggatgg tgacgcccga cgcgctgatg aaccggtcgc tgtcctttcc gaccgcggtg
      241 atgtcggcat agcgcgcaag cgcccagaag ggttcgcgaa taccgtcgtc gtgccacgcg
      301 atcggctgtt cccggcgcat gcgtgtccag acgtcgtgcg gatagccgtg cgcggcaaag
      361 ccgtcggggt cgatacaggc cggatcggcg agggggcgtc cagacatatc atcctcttcg
      421 ttagtgaacg atcgttcact agcaattcga agcccgcccc gctagggcga aatgatgacg
      481 aaggacgaag agacgaccgg ccccgcgcga cgctcgcgca tggcgccagc cgaccggcgg
      541 gccttgatcc tccagaccgc ggccgcgatg tttcgggatc atggctatgc cggggccagc
      601 atcgatgcga tcgcggcggc ttcgggaatc agcgggccgg cgatttatcg atatttcgcc
      661 ggcaaagccg aattgctgct cgcgttgctc gaagccgcgg tggcggagac ggtggccgcc
      721 atgaacgccg tgccggacag gcccgcgcag accgccgatg acctggccga cattctagcg
      781 gagcgcgcct tgagcgaggg cgccatcatt gcgctgttgc atggaacggc ggggacgatg
      841 gagcccgaag agcggttgcg ccttgataat ttgcgccggg atgcggtcgg gcgtcttacg
      901 gatgcgctgc gtgcgctccg gcccgagctc gctgcttctg aagccgaagc gcgcatcgtt
      961 gcggcgctcg cgctggtcgg gcatatcgag cgcttcctgg cgacaggagc tccggcgcaa
     1021 agtttccaga ggatcctgcg ttcgatcttg cgggcgtgat tggaaatatt acccctgctg
     1081 caagggaaac agccctgcct agaaggaata gcgcaccgag acgccataca tgcgcggttc
     1141 gttatagatg cgctgattga ttcccaatcc cgagttgatc agcgaagccc ccccgacctt
     1201 gtatgtcgtg ttggtgagat tggtgacgta gagggccaca tcgaccgggc cgccgaatgc
     1261 ctctttccag tccagcgtca ggtcgatcgt atcggtgggc ccctcgatac tttcgccgtc
     1321 atagaggttc gagccgtcgg gctgagtgat gaaaggaacc agccccaagg tgaccgagct
     1381 tttgtgggcc caattggccg aaagtaggac ctcgcccagg ctggccggca cgggaagcgt
     1441 ccagttcgcg ctgacgttca gcttgtgctt cggcgtgaag ggaaaaattc gcccgtcctg
     1501 cagcgtcggc gcattcgtcg cggaatcgcg ccgcgaataa tccagatatt cgccatattg
     1561 ggcatcggta taggcatagg cgagcatcag gttcagcgcg ctggtcggcg ccaccgtcag
     1621 ttcggcctcg acgcccttga tccttgccga ggcggcattg accagcgcgc tgacggtctg
     1681 cgtccctacc gcgaaggtct ggttgagctg cgcatcgtcc agccactgat aatagcccgc
     1741 cacattggtc ctgatgctcc aatccccgac ccggaagtcg gatttcaggc cgacttcgac
     1801 gtcgcgcaat atttcgggct ggtagcggaa gagcgacagc gcatcgggga tgggtggatt
     1861 ggcgttgagc acgggtgccg ccagcgggtt ggtgccgccg gcgcgatagc tttggcggtg
     1921 cgcaacatag atcatcgtct gctgcgtgat cttgtaatcg agcccgatcg tataggcgtc
     1981 gctgtccgcc ttggcgacga aagaggcgcc gcacgcatcg gcctgggccg gatcgggcac
     2041 caccgtgccc gtcgcgccgg tgacgcagcg accgctggtg acttgataaa gcgtcagcgc
     2101 gcggcggtcg cgggtccatc gataccccgc ggtcgcggtc aacccctcga gcccaccgcc
     2161 aaaggcatag ctgacgttgc cgaacagcgc gcgcgagctg tcgtcgatat actggttcgc
     2221 gtcggtcacc gacccgaaca ccgacgtctg atggccgtaa ttggcatcgg gggtgtcgag
     2281 atccgccccg aacaggccga tgatgaattt caggcggtca tcgagcagtt ggccctgcag
     2341 ctgcagttct tcggtcacct gccggatatt gaggctccac ggagcatcgg catcgcgcgg
     2401 gtggcagctt gccgcggtcg cgcaggtatc gaaattgagg atcaccagcc ccgaaccgtc
     2461 atagtcgaag cggttcaatt gcttgaagcg ccgatagccg aagatgttct tgacggtcag
     2521 cgcgtcgctc acctgccacg tcgtgatatt ggaaacgccc cacgaccgct tgcggttgat
     2581 ccccggggtg tcgctgaagg ttacccgggg ccccgccgca ttctgcttgt cgagcgcggc
     2641 ttccacggca ccgccgaata gcgtcgggat cagggcgttc gaattgaccg cggcgaggat
     2701 gttcgaactg ccgttggtcg tcgaccggaa catgtctgcg acgagcaggt tcgaaaaggt
     2761 cgaggagggc tcccacagaa tcgacgcgcg gccgctgaag aaactgcggt cgtcctgcca
     2821 gaccttgttg gtggcgttat aggtgaagcc gtcacgcttc agccccttgc ccgcgacgcg
     2881 cacgcgcagc gtgctgccaa gcgggacgtt gagcgcgcct tcgagcccgc gattgtcgta
     2941 attgccatat tcggcggcga tatatccgcc gaactcgtcg tcgggccggc gcggttcgaa
     3001 caggattgcg ccccctgtcg aattgcgtcc gaacaaggtt ccttgcggcc ccttgagcac
     3061 ctgaacattc tggagatcat aataatagcc ggggccgccg ccatccccga tcggcagcgg
     3121 gacctgcgca aaataggcgg gaaccgtgac ctgctggccc gagatcgagg cgcccgaccc
     3181 ggactgcccc cggatcacgt aattttcttc gtcgcggctg ctttgcgacg aggtcagcgc
     3241 gggtacgatc ttcgaaagat cgacggcatt cgagatgcgc tggctttcca gagcttcgcc
     3301 ggaaaaggcc tgcaccgcga cgggaatatc ctgaatcgcc tcctcgcggc ggcgggcggt
     3361 gacgacgatg tcggcaatgc cggcctgtcc ggtcggcgcc gcgccttctt cggcaggggc
     3421 ggtccccccg tcctgagccc aggcgggggt ggcgaatgcg ctgaacgcgg cccagccgca
     3481 cagcaggaca tgctgttttc tcatttttcc tctccctatg cgcgcatcgt tcttatgtgt
     3541 gtggggacgc gcgcagtccc ccggtcattc gttcaggcgg ggagcgatca gccctccatc
     3601 gacgggcacg gcggccccgg aaatatagga ggcgtcgtcg gagagcagga aggcgatcgc
     3661 ctttccgaac tcatcaagcc ggccgacgcg gtcgaaatgc gatagcttgt cgagataggc
     3721 ctgccggtcg ggggtgcggc tcggatgcgg cggattgccg agcaccaccg cgttgacgtt
     3781 cacgccgtgc cgcgcgacct ccttcgacac cgagctgacc agcgcgtgca gcgcgagctt
     3841 gcccgacgca taggcgctcc ccgattcctg tccgagaacc gccgagatcg aactgacgca
     3901 aaccaccttg ccatagccgc gcgcgatcat cccgggcaga agcgcgtgga gcagcatcgc
     3961 ctgggtaaag aaattcatct cgaatatcgc cctgaagtgg gcgaattcgg atgaagcgaa
     4021 ctcgccccat ggggtcatgg ggggaccacc gccggcatta ttggcaagca ggtcgacgtg
     4081 gccgtgggcc gcggtcgcct cgtcaacgaa agcgtggatg aaatcttcct ccatcacgct
     4141 gccgagatgc gtcgtcaccc ggacgccgtg cttgcgaagc gacgcggcca tttcttccaa
     4201 ccggtcgcca tcaacgtcgt tcagcgcaag cgcggcgcgc cccttggcca gttcggccgc
     4261 cgttgccgcg ccgatcccgc gtcccgcgcc ggtgaccagc gcaacgcgtc ccgcgaaacg
     4321 gttcacgctg ccttcctcgc ccgcgggtcg gagaacatcg gattggcggc gttgttggtc
     4381 catatctgat ggcgcgtgaa gcgccattcg ccggtcgcct tgctgaaggt gaacaaatag
     4441 gtcccttcgt gcaggccgtg atgccaatcg ccgtcttcct ggccgaccag cgccatctga
     4501 taatggcaaa gcgcccaggc cttttgattg tcgtcggcga cgcgcacaac ctgtgtcccg
     4561 atcaggtggc ggatttcgag gccctgtatc ttcgacaggt cgcggggcgg tgccaccacg
     4621 ccctcgcgcc tcggcagcgc cggcttggca tgcaggccga tcagctggtc gcggccctgg
     4681 acggtttcgt cgagcgtccc cctcatcaca cgctcgatat tctcgtcata aagccgctcg
     4741 agaatgtccc acccgcgact gtcgcaggca aaggcatatt cgttgatcat ctcctgaatg
     4801 cgcatcttgt cggacaggaa ttgcaccttg tcctcgatcg tcgtgcgttc gcgctcgatc
     4861 gcgggggtca ggtcgcgcca gcaggcatag gcatcgtaga gcacaatatt ctcctcgttg
     4921 atgatcgtca gatggggccg ccgcgcccgc ccgcgacgtt gatcagttcg ccgttgatga
     4981 aggcggcatc gtcgctgcac aggaaggcgg cggtggcgcc gatctcctcg ggcttgccca
     5041 gtctccccgc cagcgagagt ttcgacatga aggcgatttc cggctcggag cggttgcgtt
     5101 tcatcagcgg ggtcaggatc gggccgggcg cgatgcagtt cgcgcggatc gccggcgcat
     5161 gatcgatcgc cagcgatcgg gtcagcgcga taaccccgcc cttggccgcg acataggcgg
     5221 ggttgcggcc catccccgtc acgccggcgc ccgacgcgat gttgacgatc gaaccgcccc
     5281 ccgccgcatc gatcgccggg atcgcggcgc ggcacatcaa aaaggtcgag gtcaggttga
     5341 ggcggaccca aaagtcccat tcctcctcgt cgaaatcgac gaccgtcttg cccggccggc
     5401 tgccacccac gacattcacg agcaggtgga ggccgccgaa gcgatccgag gcgagggcga
     5461 ccgccgccgc ggcgtcgtcc gcactcgttg catccgacac caatgtttcg atcatatcag
     5521 gttcaagacc ctgccgcgta tcggccagtc ccgcctcgtc gacgtcggtt gcgacgaccc
     5581 ttgctccgcg ctcgacgaac agtcgtgcgg tcgcttgccc tatgcctgcc gccgcgcccg
     5641 tcacaatcgc caccttgccc gcaagctgca tgtcactctc cctcggcccg gatcattccg
     5701 gacgcacgaa cccgcggcgg aaagacaccg cgaaatttcc agactgtccg ggaatccttc
     5761 ccgcatcctc cgcgactctt tggtcgctaa tctttatatc tattatatat tagagctcat
     5821 tgtgcaacgc ggttcttgaa ccatcccatt gcgagtgccg cggccgccag cgcagcggcc
     5881 gcaataagaa aggcgttgtg aattcccatc atcgctcgaa gcggcgtcat gatcagcgga
     5941 ttcacgaaat cggcgatata gagcacggta aattccagtc cgatggcacg cgcgcgcaaa
     6001 tccgcgggga cccgattgag caggatgccg ctgatgtgcg gcgggatcat cccgccccct
     6061 ccgcccgcca gcaacgcccc gaacgcaagc atgcgcacat cggtcgagaa accgatgacg
     6121 gccagccccg tagccatcat caccgtcagg aatatctggg tgccccgctc ccccagccgg
     6181 gcatagaagc gtccgttaag gccgccgaaa acaccgcccg cgagcacgcc tgtggcgatg
     6241 accatcgacc gtgtcagcgg actgtcgacg ccatcctcgg ccagcaggaa cgacagctgg
     6301 atcgacgtca tgaaatatcc cacgaacagg aacatcgcga ggccgtagat cggccagagc
     6361 ttgacgagcg gacggagcga tggccgctct cctgctcgcg cggttcgcgc caccccgcgt
     6421 ggaaggccgg cgaggacgag gaagaggacc agaaaggcga aggcatagag caggaacggc
     6481 gcgcgccacc cgcccgactc ggccaacaag cccgagagca gcaagccgct gaccgcgaag
     6541 aatcctgcaa cggcgctctg gtacccgagc agcttcgctc tcgcgacacc ctcgaaccag
     6601 cttccgacga tgaccccgat cgccgttccg acctgcgcga cgaagaagcc gaggaaaaag
     6661 cgcgacacca gcaggaggcc gaggctctcg atgaaatagc cgctgatccc gagcagcccg
     6721 taactgagaa gtgccgcgat gaggaccggc cgggcgcccc accgctcgac cgacaggccg
     6781 ccggcgattc cgccgaatat caccccgatg gccggcatgg tgaggatcat ctgcgctgcg
     6841 aaagcgcccg cggtgttccc gccaaaatga tcggcgatcg aaggaagaac cggcccaagc
     6901 gctgcgaaca tcaacgccat aagcggtgcg ccgccaacca gcaccaagcg ggacattccg
     6961 ccccgccagc ctggaggaat tgctacgaat gcagcttgtg cttgtcgatc catagcccct
     7021 cccactatat taatcattta tactttatac gtcacaaata tttggctcga tcccaaatga
     7081 aacccgacct ccgatgctac tgatgagggt tgttgcttgg gcttccaggc tcattgctgt
     7141 cagcaagttt atgatgtgat cacggcgcac cgggagcggg accgtaaagc cggggcgttc
     7201 gaatttaagg gtacgacctg ctcgaaagat tgatccggag cggtgctggc cgggttacga
     7261 ttttgacgta atcgttcatc acgcgatcct ctcccacgtc attttatttt ccgagcatgg
     7321 ggaccgtgca ggcgaaggac aaatctgaaa cgggcatagg aacgtttccc aacgggcatc
     7381 ccaggcagcc gaatttggcc ggatctcgcc cgcagctatg gcaagggcgt gcttccggga
     7441 agcatgatcg acggcgtaag cggtcagctt gtaatgggct ccgtcaagca ggcccgggac
     7501 atatccggca accgtgtccg cggctgcgat gagcgcggcg tccattgtgg ggatgtagcg
     7561 gtcggttacc gaccctttcg catgtccgag cagcgccgcc accgtcgttt cagtgaaccc
     7621 gagatcattc gcgatgttcg cgaaactatg ccgcagcaca tgcggagtaa tgccgtcgag
     7681 cccgcagccg ccgagtatct tggtccaaag gccttagcgt tcccgagcgg ccgtcgcgcg
     7741 gatctcgtcg atcgggctca gcaaataaga ggcgagcgag cggcggccgg tgcggatgtc
     7801 ggcggtaacc gccatgccgg gggtgagagg aatcgccctg ccatcgcgta cgatcgtcgc
     7861 gcggtccagc gtcacccgcg cggtgtagat cagtccgcgc tgctcgtcct gtaccgcgtc
     7921 cgagctgata tgcttgagct tgcccggcac cgcgccgtag cgtgtgaagg gaaaggcctc
     7981 gaccttgagc gcgaccggtt ggccggcgcc gacgaagccg acatccttgt tggcgatcgc
     8041 cacttcggcg accagcttgc tgcgcgaagg gacgatcacc atgatcggct tggcggcctc
     8101 gacgacgccg ccgacggtat ggaccgccaa ttgcgtgacg gtgccgtcga ccgggctgac
     8161 cagccgctga aggctgctgc gtttcgccgc ttttgtcagc tcttccttgc gcagccgcgc
     8221 gtcgctctcc gccctggcga gatcggcgag gatcccggcg cgggcctctg cggcggactg
     8281 gctgaaattg ccgccggccg ccgcgatctg c
//
LOCUS       DATMSU010000253        10813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_99310, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000253 DATMSU010000000
VERSION     DATMSU010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10813
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99310"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VN034_06745"
     CDS             <1..295
                     /locus_tag="VN034_06745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV60332.1"
                     /translation="IQAYIFPGGCLMSESRFRALAEERGLAWRDVRRFGGDYAETLRQ
                     WRERFDAAVAADQLPAGFDERFVRLWRYYLQYCEGGFRGGGIDVAQVTLEKTA"
     gene            315..1502
                     /locus_tag="VN034_06750"
     CDS             315..1502
                     /locus_tag="VN034_06750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWV60333.1"
                     /translation="MRRFLAAVLLGTAAAACSAPATTQTTDQPSKDLPKDLNVLFWTQ
                     DQRDAAFRTMETVPKVVVNTVKAGGPVYPLPEGKPLDLGGDVHAYMAKQRNAGLVIVQ
                     DGKVRLEKYALGYGAAGRWTSFSVAKSFTSTLVGAAVKDGYIKSLDDKVTAYIPGLKG
                     SAYDDVSVEQLLTMTSGVKWNEDYTDPKSDVALFNLQKPVAGEDITVSYMKTLPREAP
                     AGSKWVYKTGETNLIGVLVSSATGKTLSQYLSEKIWKPFGMEQDAVWMLGATGHEISG
                     CCMSATLKDYARFGQFILNDGVAGGEKVLPDDWLASATTKQAGIDVPGRGYGYQWWTN
                     DDGSFAAQGIFGQGIFIDPKRKLVIASNGNWPTATDPEGVGAAREAFYKSVQAAVDKE
                     GGK"
     gene            complement(1536..1922)
                     /locus_tag="VN034_06755"
     CDS             complement(1536..1922)
                     /locus_tag="VN034_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60334.1"
                     /translation="MTKTILRSAIAAVIGIVVAFGLIWLAQYAGSEISPSEYDLATGE
                     ILIPIGSTIALVVGWFLATFAGGWLAMRMSGGTGAGWVVAGAVIGAALYTAVDFSDAL
                     WIMALGVAVPLAAAWLAQRAAATVIE"
     gene            complement(1984..2718)
                     /locus_tag="VN034_06760"
     CDS             complement(1984..2718)
                     /locus_tag="VN034_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60335.1"
                     /translation="MTVKSEELAGQVALVTGASRGIGEAIAESLAARGAHVVITARTA
                     GGLEELEDRIHATGGSATIAPLDLTDGDSIARLASAMAERWQQLDMMVLNAAMLGTLT
                     PVAAIDGKEFNKLLTLNLIAQQALIANFDPLLRRAPNGRLLALSSSVAREPRAYWGAY
                     AASKAAFETLVTSYGAEMRNISTLRTAILDPGGTRTQMRARAYPGEDPQSIKDPAAVG
                     EYVAKLMVEGFDSTAFHALPKVMEKA"
     gene            complement(2723..4180)
                     /gene="purF"
                     /locus_tag="VN034_06765"
     CDS             complement(2723..4180)
                     /gene="purF"
                     /locus_tag="VN034_06765"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWV60336.1"
                     /translation="MLTTHPFDDDKLREECGVFGIHGADSAAAVVALGLHALQHRGQE
                     AAGITAFDGKDFHTHRAMGHVAGNFDRDDIMRQLQGPSAIGHVRYSTTGETALRNVQP
                     LFADLSTGGFAVAHNGNISNAAALKKVLVRRGSIFQSTSDTEVIIHLVATSNYRSLLD
                     RFIDALKQVEGAYSLICLTAEGMIGCRDPLGIRPLVIGKLGDAHILASETVALDVVGA
                     EFVRSVEPGELVIVRDGQLTSHRPFAEQSARPCIFEYVYFSRPDSIVDGTSVYSVRKA
                     IGAELARENPVDADLVIPVPDSGTPAAIGYAQESGIPFELGIIRSHYVGRTFIQPGDK
                     VRHLGVKLKHNANRALIAGKRIVLIDDSIVRGTTSLKIVQMMRDAGAAEVHMRIASPP
                     TQHSCFYGVDTPERAKLLAAQMTVGQMANFINADSLAFLTIDGLYRALGEAERKHDAP
                     KYCDACFTGDYPTTLTDFDEHGLEDQFSLLAERVV"
     gene            4336..4716
                     /gene="hisI"
                     /locus_tag="VN034_06770"
     CDS             4336..4716
                     /gene="hisI"
                     /locus_tag="VN034_06770"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWV60337.1"
                     /translation="MDDQRDTTDRFLPRFDAAGLVTAIVTDADSHILLMVAHMNEEAI
                     AKTRETGQAHFWSRSRASLWRKGETSGNGLTLVEMRVDCDQDALLLRVRPAGPACHTG
                     RRSCFYRRVEADGRLSFLADNAQG"
     gene            4703..5236
                     /locus_tag="VN034_06775"
     CDS             4703..5236
                     /locus_tag="VN034_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60338.1"
                     /translation="MRKAETLLVLAAMLLAGCERPEPEPTTVDASNPLEVAARERGVV
                     RPEADSPVGVFERRHDLGRDAMCVVPDGTGKWRFAVTASFGTTLSCLATGTMAREGDK
                     WRMRFAGVEGCEALVHEEEDELRLPGRLPPQCARLCPNRASLSGLRLPRASWSADDAR
                     DLRIADRDGNMVRPCAG"
     gene            5303..5725
                     /locus_tag="VN034_06780"
     CDS             5303..5725
                     /locus_tag="VN034_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWV60339.1"
                     /translation="MTEPYGHAHIDTPDHLGREQFSITDLSTEFGVTARALRFYEDEG
                     LISPSRKGLSRIYSKRDRARLAWILRAKRTGFSLADIREMIDLYDVGDGRKLQRQVTI
                     EKCEQRIGLLQRQRDDIDSAVDELSRFIDMVKKVDAGQ"
     gene            5787..7589
                     /locus_tag="VN034_06785"
     CDS             5787..7589
                     /locus_tag="VN034_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWV60340.1"
                     /translation="MPVYRAPVQDTLFLLNDVLGIERYSNLPGFANATPDMIEAVLTE
                     AGKFCEEVLFPINQSGDLEGCTRHDDGSVTTPKGFKEAYKAYSEAGWGLLTAPEEFGG
                     QGLPHVIGFPVEEYRNAANQAFAMYPGLTQGATAAILVKGSDEQKATYVPKMIAGDWG
                     GTMNLTEPHCGTDLGLIRTRAVPNGDGSHAVTGTKIFISSGEHDLTENIIHLVLAKTP
                     DAPDSVKGISLFIVPKFLVGEDGSLGERNSLSCGSIEHKMGIHANSTCVMNYDGAKGW
                     MVGEENKGLAAMFVMMNAARLGVGIQGLGQADVAYQNAVQYAQDRRQGRALTGPKDPQ
                     EKADPLFVHPDVRRMLMDGKATVEGLRALCTWGALQVDLAHLAESEEERQRADDLVSL
                     LTPVIKGYGTDKGYEVATNAQQVFGGHGYIEEQGMSQYVRDARITMIYEGANGVQAMD
                     LVGRKLAQNGGRAIQAFFAIVDEECGRAKSDEALADFAGRLEKANGELKAATMWFMQN
                     GMANPNNVGAGAHHYMHILGIVALGSMWLMMAEAAQKALAEGRDNKAFLEAKLVTARY
                     FAERFLPDAGSLRRKIEAGSDAMMALTPEQFAAA"
     gene            7636..9129
                     /locus_tag="VN034_06790"
     CDS             7636..9129
                     /locus_tag="VN034_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWV60341.1"
                     /translation="MGTELSPIVVEGRNCWRIERADKARMIVDAADYYKLLKQLMADA
                     KERILLIGWDFDPRIALTPDDKGKGEPLGDYLLDLARDKPDRDIDILRWNFGGLKQFA
                     VPRILSMVVRWRLTRAISFRLDSAHPVGCSHHQKVAVFDDHLAVCGGIDVGSRRWDTR
                     DHKDGDPHRTAPDGNAYMPWHDSTMILAGPVGNALAELGNERWQRATKKPLREVEGDG
                     ENWPDDLDPDFEGIDVAISRTRAEYEDYGEIREIEQLYLDMIAAAKRFIYFENQYFTC
                     AKIAAAIAERLDEDDPPEFVMVMPETADGWLEQMAMDAARVQLVREIAKAKHGDRLKV
                     YYPRTAKGEPIYVHAKTAVIDDRMIRVGSANMNNRSMGLDSECDVTIDTALPANAGVE
                     PVIRRLRESLIAEHLDVDPAEVARRFDEAGSLIATIEALRGDGRSLELLDLTKPGPFD
                     EFIADNELLDPTSPDDMFESLTERGLRKSWHRGKSWMERHRPFRRKS"
     gene            complement(9126..9977)
                     /locus_tag="VN034_06795"
     CDS             complement(9126..9977)
                     /locus_tag="VN034_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWV60342.1"
                     /translation="MRLRSLLFVPGDRPERFAKAAASGADAIILDLEDSVSLVNKEAA
                     RAAVAEYLAGEREVITLVRVNPLDGHMTAADVAAIAGARPDAIMLPKAEGAPSILQLD
                     TILRSESARDASLPAVLPIATETPAAIFTLGSYREVKDRLLGLTWGAEDLPAAIGATT
                     SRNDDGNYTAPYEMARALTLFAAHGANTAAIDTVFPAIKDETGLAAYAARARRDGFTG
                     MMAIHPSQVGPINAAFTPSTEEVARAQAIVDAFAANPGVGVLQVDGKMVDAPHLKQAH
                     HILSLAD"
     gene            complement(9977..10429)
                     /locus_tag="VN034_06800"
     CDS             complement(9977..10429)
                     /locus_tag="VN034_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWV60343.1"
                     /translation="MAGKYFGEWQIGDTLTHDIRRTVTETDNLLFTTMTHNPQPLHLD
                     IEAAKASEFGQILVNGTFTFSLMVGLSVGDTTLGTLVANLGYDKLVMPKPVFIGDTLR
                     AESEVIGLKESKSRPNAGIVTFLHRAINQRGEIVCQCERSALIKKKDA"
     gene            complement(10561..>10813)
                     /locus_tag="VN034_06805"
     CDS             complement(10561..>10813)
                     /locus_tag="VN034_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWV60344.1"
                     /translation="DASAGGAAGDGAILSPMPGKVIAVDVAAGDKVTKGQKLLTLEAM
                     KMEHSLTAPFDGIVAELNATAGAQVQVEALLARIEKSGD"
BASE COUNT         1982 a         3327 c         3609 g         1895 t
ORIGIN      
        1 catccaggcc tatatcttcc ccggcggctg cctgatgtcc gaaagccgct ttcgcgcgct
       61 ggcggaggaa cgcgggctcg cgtggcgcga cgtgcggcgg ttcggcggcg attatgccga
      121 gacgctgcgc cagtggcgcg aacgcttcga cgcggcggtg gccgccgatc agcttccagc
      181 ggggttcgac gaacgcttcg tccggctctg gcgctattat ctgcaatatt gcgaaggggg
      241 ctttcgcggc ggcggcatcg acgtcgcgca ggtcacgctc gaaaagacag cctgaaaaca
      301 ggggaggaca tcatatgcga agatttttgg cggcggtgct gctcggcacc gcggcggcgg
      361 catgcagcgc gcccgcgacg acgcaaacca ccgatcaacc gtcaaaggat ctgccaaagg
      421 atttgaatgt gctgttctgg acccaggatc aacgcgacgc cgctttccgc acgatggaga
      481 ccgttccgaa agtcgtcgtc aacacggtga aggcgggcgg gccggtctat ccgctgccag
      541 agggcaagcc gctcgacctt ggcggcgatg tccacgccta tatggcgaag cagcgcaacg
      601 cggggctggt cattgtccag gacggcaagg tccgcctcga gaaatatgcg ctcggttatg
      661 gcgccgcggg gcgttggacg agcttctcgg tcgccaagag cttcacctcg accctcgtcg
      721 gcgccgcggt gaaggacggc tatatcaaga gcctcgacga caaggtcacc gcctatattc
      781 cggggctcaa ggggtcggcc tatgacgatg tctcggtaga gcagttgctg acgatgacct
      841 cgggggtcaa atggaacgag gattataccg atcccaagtc cgacgtcgcc ttgttcaatc
      901 tgcaaaaacc cgtcgccggc gaggacatta ctgtcagtta tatgaagacc ttgccacgcg
      961 aagctcctgc ggggtcgaag tgggtataca agaccggcga gaccaatttg atcggcgtgc
     1021 tcgtgtcgag cgcgacgggc aagacgctct cgcaatatct gtccgagaag atctggaagc
     1081 cgttcggcat ggagcaggac gcggtatgga tgctcggcgc gacggggcat gagatcagcg
     1141 gctgctgcat gtcggcgacc ctcaaggatt atgcgcgctt cggccagttc atcctgaacg
     1201 acggcgtcgc gggcggcgag aaagtgctgc ccgacgactg gctcgcgtcg gcaacgacga
     1261 agcaggcggg aatcgacgtt ccgggtcgcg gctatggcta tcaatggtgg acgaacgacg
     1321 acggcagttt tgccgcgcag ggcatcttcg ggcagggcat cttcatcgac ccgaagcgga
     1381 agctggtgat cgcgtcgaac ggcaactggc cgaccgccac cgatcccgaa ggcgtcggtg
     1441 cggcgcgtga ggccttctac aagagcgtgc aggcggcggt ggataaggaa ggcggcaaat
     1501 aaagaacgtc gcccccgcct tcgcgggggc gacgtttatt cgatgacggt cgccgccgct
     1561 cgctgcgcga gccaggctgc agctagcggt acggcgacgc ccagcgccat gatccacaaa
     1621 gcgtcggaaa aatcgacggc ggtatagagg gcggcgccga taaccgcgcc cgcgaccacc
     1681 catccggcgc cggttccgcc ggacatccgc atcgcgagcc agcctcctgc aaaggtcgcg
     1741 agaaaccagc caacgacaag agcaatggtc gatccgatcg ggatcaatat ttcgcccgtc
     1801 gcgaggtcat attcggaggg cgaaatttcg ctcccggcat attgcgccag ccagatcagc
     1861 ccgaaagcga cgacgatgcc gatcacggcg gcgatcgccg atcgcaaaat agtcttggtc
     1921 attgttcccg ccctcgcaaa ccctcttatg cgagtgtctt agcgcaggaa aacgaagatg
     1981 gcgttaagct ttttccatga ccttcggcag cgcgtggaaa gcggtgctgt cgaagccttc
     2041 gaccatcagc ttcgcgacat attcgcccac tgccgccggg tccttgatgc tctgcggatc
     2101 ctcgccggga taggcgcgcg cgcgcatctg ggtgcgggtg ccgccggggt cgagaatcgc
     2161 ggtgcgcagg gtggagatat tgcgcatttc ggcgccatag ctcgtcacca gcgtctcgaa
     2221 cgcggccttc gacgcggcat aggcacccca ataggcgcgc ggctcgcgcg cgacgctgct
     2281 cgacagcgcg agcaggcggc cgttcggggc gcggcgcagc agcggatcga aattggcgat
     2341 cagcgcctgc tgcgcgatca ggttcagcgt cagcagcttg ttgaattcct tgccgtcgat
     2401 cgccgcgacg ggggtcagcg tgccgagcat cgcggcgttg agcaccatca tgtcgagctg
     2461 ctgccagcgt tcggccatcg cactcgcgag ccgcgcgatg ctgtcgccgt cggtgaggtc
     2521 gagcggcgcg atcgtcgcgc tgccgcccgt agcatggatg cggtcttcga gttcttcgag
     2581 cccgcccgcc gtgcgcgcgg tgatcacgac atgcgcgccg cgtgccgcga gcgattcggc
     2641 gatcgcttcg ccgatcccgc ggctcgcccc ggtgacgagc gcgacctggc ccgccagttc
     2701 ttcagatttc acagtcatta ggtcagacaa cccgttcggc aagcagcgaa aattgatctt
     2761 ccagtccatg ctcgtcgaaa tcggtcagcg tggtcggata gtcgccggtg aagcaggcgt
     2821 cgcaatattt gggggcgtca tgcttgcgct cggcttcgcc cagcgcgcga tagaggccgt
     2881 cgatcgtcag gaaggcgagg ctgtcggcgt tgatgaagtt cgccatctgg ccgaccgtca
     2941 tctgcgccgc gagcagtttc gcgcgttcgg gcgtgtcgac gccatagaag cagctatgct
     3001 gggtcggcgg gctcgcgatc cgcatatgta cttcggcggc gccggcatcg cgcatcatct
     3061 gcacgatctt caggctcgtc gtaccgcgca cgatcgaatc gtcgatcagc acgatgcgct
     3121 tgcccgcgat cagcgcgcgg ttggcgttgt gcttcaattt gacgccgagg tggcggacct
     3181 tgtcgcccgg ctggatgaag gtgcgcccga cataatgcga gcggatgatg ccgagttcga
     3241 acggaatgcc cgattcctgc gcatagccga tcgccgcggg ggtgcccgaa tcggggacgg
     3301 ggatgacgag gtcggcgtcg actggatttt cgcgcgcgag ttcggcgccg atcgccttgc
     3361 gcaccgaata gacgctcgtc ccgtcgacga tcgaatcggg gcgcgagaaa tagacatatt
     3421 cgaagatgca cggccgcgct gattgctccg cgaaggggcg gtgcgaagtg agctggccgt
     3481 cgcgaacgat aacgagctcg ccgggttcga ccgagcgcac gaattcggcg ccaaccacat
     3541 ccagcgcgac ggtttcggag gcgaggatat gcgcgtcgcc gagcttgccg atgacgagcg
     3601 ggcggatgcc cagcggatcg cggcagccga tcatgccctc ggcggtcaag cagatcagcg
     3661 aataggcgcc ttcgacctgc ttcagcgcgt cgatgaagcg gtcgagcaag gaccgatagt
     3721 tcgacgtcgc gacgaggtgg atgatcactt cggtatcgct cgtcgactga aagatcgaac
     3781 ctcggcgaac gagcaccttc ttcagcgccg ccgcgttgga gatattgccg ttgtgtgcaa
     3841 ccgcgaagcc gcccgtcgac aggtcggcga acagcggctg gacattgcgc agcgcggtct
     3901 cgcccgtcgt cgagtaacgg acatggccaa tcgccgaagg accctgcaac tggcgcatga
     3961 tgtcgtcgcg gtcgaaattg cccgcgacat ggcccatcgc gcggtgggtg tggaaatcct
     4021 tgccgtcaaa ggcggtgatg cccgcggctt cctgtccgcg gtgctgcagg gcgtggagcc
     4081 ccagcgcgac aaccgcggcg gcgctgtcgg cgccatggat accaaatacg ccgcactcct
     4141 cgcggagctt gtcgtcgtcg aaaggatggg tcgtcagcat gtcattccag ggtcggggcc
     4201 aggggcaggg gggcagtgac tgcggcgcat atagggacgg gaatcgcaaa tgtcgccccc
     4261 ctaatcacgc atttgtaaca cagggcgcga cggacggggt ggcagcggcc gcaatcctgc
     4321 tctagagcgc gctttatgga tgatcagcgc gacacgaccg accgatttct tccgcgtttc
     4381 gatgccgcgg ggctggtgac cgcgatcgtc accgatgccg acagccatat cctgctgatg
     4441 gtcgcgcata tgaacgaaga ggcgatcgcg aaaacgcgcg agaccgggca agcgcatttc
     4501 tggtcgcgtt cgcgcgcgtc gctgtggcgc aagggcgaaa cgtcgggcaa cggcctgacg
     4561 ctcgtcgaga tgcgcgtcga ttgcgatcag gacgcgctgc tgctgcgcgt gcggcccgcg
     4621 ggtcccgcat gccacaccgg gcgccgctcc tgcttttatc gccgcgtcga ggccgacggc
     4681 cgcctgagct ttctggcgga caatgcgcaa ggctgagacc cttctggtgc tggcggcgat
     4741 gctgctcgcg gggtgcgagc ggcccgagcc tgaaccaacg accgtcgacg ccagcaatcc
     4801 gttagaggtc gcggcgcgcg agcgcggtgt cgttcgcccc gaagcggact cgccggtcgg
     4861 cgtattcgag cgcaggcacg acctcggccg cgacgcgatg tgcgtcgttc ccgacgggac
     4921 cgggaaatgg cgctttgcgg tcaccgcctc cttcggaacg accctgtcct gtctcgcgac
     4981 cggaacgatg gcgcgcgagg gcgacaaatg gcggatgcgc ttcgccggtg tggagggctg
     5041 cgaggcgctg gtgcatgagg aagaggatga attgcgcctg ccgggccgcc tgccgccgca
     5101 atgcgcgcgc ctgtgcccga accgcgcctc gctttcgggt ttgcggctgc cgcgcgcaag
     5161 ctggtcggcc gatgatgcga gggacttgcg gatcgccgat cgggacggca atatggtgcg
     5221 tccctgcgcg ggctgacccg ccccgaattc gccccatccg gcgctattga cgttcacgtc
     5281 aacggaaact agtttcagcg gtatgaccga accctacggc cacgcccata tcgatacgcc
     5341 cgatcatctg gggcgcgagc aattcagcat caccgacctg tcgaccgagt tcggggtcac
     5401 cgcgcgcgcg ctgcgtttct atgaggacga agggctgatc agcccgtcgc gcaagggatt
     5461 atcgcgcatc tattcgaagc gtgatcgcgc gcggctcgcg tggatcctgc gcgcgaagcg
     5521 gacggggttc agcctcgccg acattcgcga gatgatcgac ctctacgacg tcggcgacgg
     5581 ccgcaaattg cagcgtcagg tgacgatcga gaaatgcgaa cagcgcatcg gcttgctgca
     5641 acgccagcgt gacgatattg acagcgctgt agatgagctg tcacgtttca tcgacatggt
     5701 taaaaaggtc gacgccggcc aataggtcga tctgagcctc tccccaggca agtgacatca
     5761 tcagctgctt ttcagaagga tttcccatgc ccgtctatcg cgctcccgtg caagacacgc
     5821 tgttcctcct caacgacgtg ctcgggatcg agcgttattc gaacctgccc ggtttcgcca
     5881 atgcgacccc cgacatgatc gaggcggtac tgaccgaggc gggcaagttc tgcgaggaag
     5941 tcctgtttcc gatcaaccag tcgggcgacc tcgaaggctg cacgcgccac gacgatggct
     6001 cggtgacgac gccgaagggc ttcaaggaag cgtataaggc ctattcggaa gccggctggg
     6061 gcctgctgac cgcgcccgag gagttcggcg ggcaggggct gccgcatgtc atcggtttcc
     6121 cggtcgagga atatcgcaat gccgccaacc aggcgttcgc catgtatccg ggactgacgc
     6181 agggcgcgac cgcggcgatc ctcgtcaagg ggtcggacga acagaaggcg acctatgttc
     6241 ccaagatgat cgcgggcgac tggggcggga cgatgaacct gaccgaaccg cattgcggca
     6301 cggacctcgg ccttatccgc acccgcgcgg tgccgaacgg cgacggcagc catgccgtca
     6361 cgggcaccaa gatatttatt tcgtcgggcg agcacgacct gaccgagaat atcattcacc
     6421 tcgtcctcgc gaagactccc gatgcgcccg acagcgtcaa gggcatctcg ctgttcatcg
     6481 tgcccaagtt cctcgtgggc gaagacggct cgctcggcga gcgcaacagc ctctcgtgcg
     6541 gatcgatcga gcacaagatg ggcatccacg ccaattcgac ctgcgtgatg aattatgacg
     6601 gcgcgaaagg ctggatggtc ggcgaggaga ataagggtct cgccgcgatg ttcgtgatga
     6661 tgaacgccgc gcgcctcggc gtcggcatcc aggggctcgg ccaggccgat gtcgcctatc
     6721 agaacgccgt ccaatatgcg caggatcgcc gccagggccg cgcgctgacc ggtccgaagg
     6781 acccgcagga aaaggctgac ccgctgttcg tccaccccga cgtgcgccgg atgctgatgg
     6841 acggcaaggc gacggtcgag gggctgcgcg cgctctgcac ctggggggcg ctgcaagtcg
     6901 acctcgcgca tctcgccgag agtgaagagg agcgccagcg cgccgacgat ctcgtcagcc
     6961 tgttgacccc ggttatcaag ggctatggca ccgacaaggg ctatgaggtc gcgaccaatg
     7021 cgcagcaggt gttcggcggc catggctata tcgaagaaca gggcatgtcg cagtatgtcc
     7081 gcgatgcgcg catcacgatg atctatgaag gcgcgaacgg ggtgcaggcg atggacctcg
     7141 tcggccgcaa gctcgcgcag aatggcgggc gcgcgatcca ggctttcttc gcgatcgtcg
     7201 acgaggaatg cggccgcgcc aagagcgatg aggcgctcgc cgatttcgcg ggccggctcg
     7261 aaaaggccaa tggcgagctc aaggccgcga cgatgtggtt catgcagaat ggcatggcga
     7321 accccaacaa tgtcggcgcg ggcgcgcatc actatatgca catattgggg atcgtcgcct
     7381 tgggctcgat gtggcttatg atggccgaag cggcgcagaa ggcgctcgcc gaagggcgcg
     7441 acaataaggc gttcctcgaa gcgaaactcg tcaccgcgcg ctatttcgcc gaacgcttcc
     7501 tgcccgacgc gggttcgctc cgccgcaaga tcgaggcggg cagcgacgcg atgatggccc
     7561 tgactcccga acagttcgcc gcggcttgat ttttagccct tgcaaccatc gccgccatca
     7621 tgcgtggtgg cggcgatggg gacggagcta tcaccaatcg tcgtcgaagg ccgcaattgc
     7681 tggcggatcg agcgcgccga caaggcgcgg atgatcgtcg atgccgccga ttattataag
     7741 ctgctcaaac aattgatggc ggatgcgaag gaacgcatcc tgctcatcgg ttgggatttc
     7801 gacccacgca tcgcgctcac gcccgacgac aagggcaagg gcgagccgct cggcgactat
     7861 ctgttggatc tcgcgcgcga caaacccgat cgcgacatcg atatccttcg ctggaatttc
     7921 ggcggactga agcagttcgc ggtgccgcgc atcctgtcga tggtcgtgcg ctggagattg
     7981 acgcgcgcga tcagcttccg cctcgacagc gcgcaccccg tcggatgcag ccatcaccag
     8041 aaggtcgcgg tgttcgacga ccatcttgcc gtctgcggag gcatcgacgt cggatcgcgt
     8101 cgctgggata cccgcgacca caaggatggt gacccgcacc gcacggcgcc cgacggcaat
     8161 gcctatatgc cgtggcacga cagcacgatg atcctggcgg gccccgtcgg caacgcgctc
     8221 gccgagcttg gcaacgagcg ctggcagcgc gcgaccaaga agccgctgcg cgaggtcgag
     8281 ggcgacggag agaattggcc cgatgatctc gatcccgatt tcgaggggat cgatgtcgcg
     8341 atatcgcgca cgcgcgccga atatgaggac tatggcgaaa tccgcgagat cgagcagctt
     8401 tatctcgaca tgatcgcggc ggcgaaacgc ttcatctatt tcgaaaacca gtatttcacc
     8461 tgcgccaaga tcgccgccgc catcgccgag cgattggacg aggatgatcc gcccgaattc
     8521 gtgatggtga tgcccgagac cgccgacggc tggctcgaac agatggcaat ggacgctgcg
     8581 cgcgtgcagc tcgtccgcga aatcgccaag gcgaagcatg gcgaccggct gaaggtctat
     8641 tatccgcgca cggcgaaggg cgaacccatt tacgttcacg ccaagaccgc ggtgatcgac
     8701 gaccggatga tccgcgtcgg atccgccaat atgaacaatc gctcgatggg gctcgacagc
     8761 gaatgcgacg tgacgatcga taccgccttg cccgctaatg cgggggtaga gccggtgatc
     8821 cggcgcctac gcgaatcgct gatcgccgag catctggacg tcgatccggc cgaggtcgcc
     8881 cgccggttcg atgaggcagg atcgctgatc gcgacgatcg aggcactccg cggcgacggc
     8941 cgttcgctcg aactgctcga cctgaccaag ccggggccgt tcgacgaatt catcgctgac
     9001 aatgaactgc tcgatccgac gagccccgat gacatgttcg aaagcctgac cgagcgcggg
     9061 ctcaggaaaa gctggcatcg cggcaagtca tggatggagc ggcatcggcc gtttcggcgc
     9121 aagagctagt cagcgagcga cagaatatga tgcgcttgct tgaggtgcgg cgcgtcgacc
     9181 atcttgccgt cgacctggag cacgccgacc ccggggttgg ccgcgaaagc atcgacgatc
     9241 gcctgcgcgc gcgccacctc ttcggtcgag ggggtgaagg cggcgttgat cggcccgact
     9301 tgcgagggat ggatcgccat catgcccgta aagccgtcgc gccgtgcgcg ggcggcatag
     9361 gcggcgaggc cggtctcgtc cttgatcgcg ggaaacacgg tgtcgatcgc ggcggtgttc
     9421 gcgccatgcg cggcgaacag ggtcagcgcg cgcgccattt catagggcgc tgtgtaattg
     9481 ccatcgtcgt tccggctcgt cgtcgcgccg atcgccgcgg gcagatcctc ggcgccccag
     9541 gtcaggccga gcaggcggtc cttcacttcg cggtagctgc cgagggtgaa gatcgcggcg
     9601 ggcgtctcgg tcgcgatggg caggacggcg ggcaacgagg catcgcgcgc ggattcgctg
     9661 cgcaatatgg tgtcgagctg gaggatgctc ggcgcgcctt cggccttggg cagcatgatc
     9721 gcgtcggggc gggcgccggc gatcgcggcg acatcggcgg cggtcatatg gccgtcgagc
     9781 gggttgacgc gcaccagcgt gatgacctca cgctcgccag ccagatattc cgcgactgcg
     9841 gcgcgcgccg cctccttgtt cacgagcgac accgaatcct cgaggtcgag gatgatcgcg
     9901 tcggcgcccg acgccgccgc cttggcgaag cgttcggggc ggtcgccggg gacgaagagg
     9961 agggaacgga ggcgcatcag gcgtcctttt tcttgatgag cgcggagcgt tcgcactggc
    10021 agacgatctc gccgcgctgg ttgatcgcgc gatggaggaa ggtcacgata cccgcattcg
    10081 gccgcgattt cgattccttg aggccgatca cttcgctttc cgcgcgcaac gtgtcgccga
    10141 tgaacaccgg cttcggcatc acgagcttgt catagccgag gttcgcgaca agcgtgccga
    10201 gcgtcgtatc gccgaccgac agcccgacca tcaggctgaa ggtgaaggtg ccattgacga
    10261 ggatctgccc gaattccgac gccttcgccg cctcgatatc gaggtggagc ggctgcggat
    10321 tgtgcgtcat cgtcgtgaag agcagattgt cggtttcggt gacggtgcgg cggatgtcgt
    10381 gcgtcagcgt gtcgccgatt tgccattcgc cgaaatattt gcctgccata tttccactcc
    10441 aaaatttgtc atgctgaact tgtttcagca tctatggcct gttctctcga ttggcgcggc
    10501 gtggttgtcg aggccgggcc atggaccctg aaacgagttc agggtgacga agaacaaaat
    10561 ctaatcgcca ctcttctcga tccgcgcgag cagcgcctcg acctgcacct gcgcgcctgc
    10621 cgtcgcattg agttcggcaa cgatgccgtc gaagggcgcg gtcaggctgt gctccatctt
    10681 catcgcttcg agcgtcagca atttctgtcc cttggtcacc ttgtcgccgg cagcgacatc
    10741 gaccgcgatc accttgccgg gcatcggcga gaggatcgcg ccatcgcccg ccgcgccgcc
    10801 cgcgctcgcg tcg
//
LOCUS       DATMSU010000254         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_156294,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000254 DATMSU010000000
VERSION     DATMSU010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156294"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VN034_06810"
     CDS             complement(<1..380)
                     /locus_tag="VN034_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWV60345.1"
                     /translation="MDIAAKIAELGLELPKPAAPVAAYVPVVEQGGMLYVSGQLPFRD
                     GQVVTGRLGDDMDVAGGQDAAQRVALMLVAQIGQALGGDWSRVERVVKLGVFVNSAPS
                     FTDQAKVANGASELFETLFGEAGR"
     gene            complement(395..1036)
                     /locus_tag="VN034_06815"
     CDS             complement(395..1036)
                     /locus_tag="VN034_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWV60346.1"
                     /translation="MNRPLVITDCDEVLMHMVVPFAEWVDTEHGVIFRIEDASFANAL
                     KRKECGTPLEAAEVWPLLDGFFRTEMTRQYPIPGALAAMAAIGAEADIVVLTNVGPEH
                     QQARIEQLALHEFHAPVIGSRGGKGEPVRRLIEQYQPSIAVFIDDLAGHHQSVAEAAP
                     DVWRLHLIGEPAIAGKIAPAKYAHARIDDWNDARDWILARLAENIPAPAAEPV"
     gene            complement(1033..1296)
                     /locus_tag="VN034_06820"
     CDS             complement(1033..1296)
                     /locus_tag="VN034_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HWV60347.1"
                     /translation="MLEDDAALALQALGWILSDEPRAERLLALTGLAPDELRASLGEQ
                     ATLAAILAFLAGHENDLVACADALQVPPASIAAAAQRLEGTPA"
     gene            1381..1743
                     /locus_tag="VN034_06825"
     CDS             1381..1743
                     /locus_tag="VN034_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV60348.1"
                     /translation="MGKTILVVEDNELNLRLFCDLLNAHGYSAHPVRDGRDALAKARE
                     ITPDLIIMDIQLPHVSGLDLIGQMKADLTLRAVPIMAVTAYAGKGDEEQIKAAGAEAY
                     VSKPISVIKFIESVGVFA"
     gene            complement(1806..2495)
                     /locus_tag="VN034_06830"
     CDS             complement(1806..2495)
                     /locus_tag="VN034_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWV60349.1"
                     /translation="MTDIVEDHIAAALDREGWAVLPRLLNDAECDETAGLYAREEGFR
                     SHVHMARHGFGRGEYRYFAYPLPPLVEALRGMLYARLAPIANRWHERMGMAARFPADH
                     AGFLARCHDAGQRRPTPLLLRYGASDYNCLHQDLYGEHVFPLQVAVLLSAPGDDFTGG
                     EFVLTEQRPRMQSRAAVVPLAKGDAVVFAVNARPVRGSRGDYRVAMRHGVSSIRTGQR
                     HTLGVIFHDAA"
     gene            2472..2759
                     /locus_tag="VN034_06835"
     CDS             2472..2759
                     /locus_tag="VN034_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60350.1"
                     /translation="MIFDNVRHADAIALAARGRAPFLAVKPSPHAPEKITGLPDGCEI
                     VTSPVHSNAKKGGRNEIYDRSMPSRQVFSLRALRPLGPEASVIRFSNWIAP"
BASE COUNT          504 a         1007 c          946 g          501 t
ORIGIN      
        1 tggcggcccg cttcgccgaa cagcgtttcg aacagctccg acgcgccgtt ggcgaccttc
       61 gcctggtcgg tgaagctcgg cgcgctgttg acgaacacgc cgagcttgac gacgcgctcg
      121 acgcgcgacc agtcgccgcc caaggcctgc ccgatctgcg cgacgagcat cagcgcgacg
      181 cgctgcgccg catcctgccc gcccgcaacg tccatatcgt cgccgagacg cccggtgacg
      241 acctgcccat cgcggaaggg cagctggccg ctgacataga gcatgccccc ctgctcgacg
      301 acgggcacat aggcggcgac cggcgcggcg ggcttgggaa gctcaaggcc gagttcggcg
      361 atcttggcgg cgatatccat gggcgtgttc cttcttaaac cggctcggcg gccggggcgg
      421 ggatgttttc ggcgaggcgc gccaatatcc agtcgcgcgc gtcgttccaa tcgtcgattc
      481 gcgcatgggc atatttcgcc ggggcgatct tgccggcgat cgccggctcg ccgatcaggt
      541 gcagccgcca cacgtcgggc gcagcctcgg cgaccgactg gtgatggccg gcgaggtcgt
      601 cgatgaacac cgcgatcgag ggctgatatt gctcgatcag gcggcgcacc ggctcgccct
      661 tgccgccgcg gctgccgatc accggcgcgt ggaattcgtg gagcgcgagc tgctcgatcc
      721 gcgcctgctg atgctcggga ccgacgttgg tgaggacgac gatatcggct tcggccccga
      781 tcgccgccat cgcggccagc gcgccgggga tcggatattg ccgcgtcatt tcggtgcgga
      841 agaagccgtc gagcagcggc catacctcgg ccgcctccaa tggcgtgccg cattccttgc
      901 gcttcagcgc attggcgaag ctcgcatcct cgatgcggaa gatcacgcca tgttcggtgt
      961 cgacccattc ggcaaagggc acgaccatgt gcatcagcac ctcgtcgcaa tcggtgatga
     1021 cgagcgggcg gttcatgcgg gggttccttc gagtctttgc gccgcggcgg cgatgctggc
     1081 cggtggcacc tgcaacgcat cggcgcaggc gacaaggtca ttctcgtggc cggcgaggaa
     1141 agcgaggatc gccgcgagcg tcgcctgctc gccgagtgag gcgcgcagtt cgtcgggcgc
     1201 gaggccggtc agcgcgagca accgttcggc gcgcggttcg tcgctcaaaa tccacccgag
     1261 cgcctgaagc gccagcgccg catcatcttc cagcaagccc gatcccttcg cgatgcaatt
     1321 gtgtcgtgcg gccgattcgc ctaaacggac cgcgcacgca aggtggggaa cggaacgcac
     1381 atgggcaaga ccatcctggt cgtcgaggat aatgaactca acctccgcct cttctgcgac
     1441 ctcctcaacg cccatggcta cagcgcgcat ccggtgcgcg acgggcgcga tgcgctggcg
     1501 aaggcgcgcg aaatcacccc cgacctgatc atcatggata tccagctgcc gcacgtcagc
     1561 gggctcgatc tgatcgggca gatgaaggcc gacctgacgc tgcgcgccgt gccgatcatg
     1621 gcggtgaccg cctatgcggg gaagggcgac gaggaacaga tcaaggcggc gggggccgaa
     1681 gcctatgtct cgaaaccgat ttcggtgatc aagttcatcg aaagcgtcgg ggtgttcgcc
     1741 taggacttgt tgcggccagt tggcggccgc cggttatcgg gcccttctcg acctgcgcag
     1801 cgacctcatg ccgcgtcgtg gaagatcacc cccagcgtgt gccgctgtcc cgtacggatg
     1861 ctacttaccc cgtggcgcat cgccacgcga tagtcgccac gactcccgcg caccggccgc
     1921 gcgttcaccg cgaagacgac cgcgtcaccc ttcgccagcg gcacaacggc ggcgcgtgac
     1981 tgcatccgcg gccgctgttc ggtcagcacg aactcgcccc cggtgaaatc gtccccgggt
     2041 gccgacagca gcaccgcgac ctgcaacgga aagacatgct cgccatagag atcctggtgc
     2101 aggcaattat aatcgctcgc gccatatcgc agcagcaagg gcgtcggccg gcgctggccc
     2161 gcatcgtggc accgcgcgag gaacccggca tgatcggccg gaaagcgcgc cgccatcccc
     2221 atccgttcgt gccagcggtt cgcgatcggt gcgagccgcg cgtaaagcat cccgcgcaaa
     2281 gcctcgacca gcggcggcag cggataggcg aaatagcgat attcgccgcg tccgaagccg
     2341 tgccgcgcca tatgcacatg gctgcgaaaa ccctcctccc gcgcatagag cccggcggtt
     2401 tcgtcgcatt cggcgtcgtt cagcagccgg ggcagcacgg cccagccttc gcgatcgagc
     2461 gcggcggcga tatgatcttc gacaatgtcc gtcatgccga tgccatcgca ttggccgcgc
     2521 gcggccgcgc cccgtttctt gctgtcaaac catcgccgca tgcgcccgaa aaaatcacag
     2581 gcttgccaga tggatgcgag attgttacct ctcccgtcca ttccaatgcg aagaaaggag
     2641 ggcgtaatga aatttatgat cgcagcatgc cctcccggca ggttttcagc cttcgagcgc
     2701 ttcgaccact aggtcctgaa gcctccgtga tccggttttc gaactggatc gccccctgac
     2761 ataacgcttt ggagacggcg ccccggcgcc ggttcatcat atgacatcga cacccgacaa
     2821 gggccgcgcg gattcgatcc gccgcgtcct cgatttcacc tttcgccact gggccgggca
     2881 gcgccgacgc gccgccttca tcgcgctgtt catcaccgcg gcgacgctca cggagatttt
     2941 cgtgcctctg ttcgcggg
//
LOCUS       DATMSU010000255         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_70770, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000255 DATMSU010000000
VERSION     DATMSU010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70770"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="crtY"
                     /locus_tag="VN034_06840"
     CDS             complement(<1..799)
                     /gene="crtY"
                     /locus_tag="VN034_06840"
                     /EC_number="5.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045436 - lycopene beta cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene beta-cyclase CrtY"
                     /protein_id="HWV60351.1"
                     /translation="MAGQRIDKCDIAIVGGGLAGGLAVLALAAKRPDLDVRLIEPGPV
                     GGNHIWSFFDSDIAKKDRWLVAPLVRHHWPRYDVRFPSHERRLRMGYKSITGEALAEA
                     VAAALPEGHIIADKAKHVAPDHLLLARGGRLSAKHVIDARGAGKFAGLDCGWQKFVGQ
                     ALTVKGGHGVEYPVVMDATVEQLDGYRFVYLLPFDTETLFVEDTYYSDDADLDAGAVR
                     ERIAAYAAAQGWRVKAATREESGVLPVVMAGDFERLWPASDRTARIGV"
     gene            874..1632
                     /locus_tag="VN034_06845"
     CDS             874..1632
                     /locus_tag="VN034_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWV60352.1"
                     /translation="MTGWTILFSALAMTAIVGVRYLITSGAFAFATKWKHPGLYRGLE
                     PQMKREIGWSLASAAIYGVPAGIVAWGWQEHGWTRIYTDIDAFPLWYLPVSLFAYLLL
                     HDTWFYWTHRWMHRPALFRAAHAVHHASRPPTAWAAMSFHPLEAITGAVVIPALVFLV
                     PIHVGVLGLVLAIMTVMGVGNHMGWEMFPRALVHGPAGQWLITATHHQAHHAAYRGNY
                     GLYFRFWDKACGTDIGLGSFADAAGRREPAAADG"
     gene            1592..1987
                     /locus_tag="VN034_06850"
     CDS             1592..1987
                     /locus_tag="VN034_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HWV60353.1"
                     /translation="MLLAAASPPPPTVEVSVTGLRSAKGQILVCLTTNRKAFPDCSKD
                     AGSVRMAVKAAEAENFAVHAPAAGTYAIAVVHDENGNNKLDKAIFLPKEGFGFSRNPT
                     ITVGPPSFKSASFAVAGDMRQSIRMKYML"
BASE COUNT          415 a          821 c          762 g          439 t
ORIGIN      
        1 gcacgccgat ccgcgcggtg cgatcggacg cgggccacaa tcgctcgaaa tcgcccgcca
       61 tcaccaccgg cagcacgccg ctctcctcgc gcgtcgccgc cttgaccctc cagccttgcg
      121 ccgcggcata ggcggcgatc cgttcgcgca ccgcccccgc atcgaggtcg gcgtcgtcgc
      181 tgtaatacgt gtcctcgacg aacaaggtct cggtatcgaa gggcagcaga tagacgaagc
      241 gatagccgtc cagttgctcg acggtcgcgt ccatcacgac cggatattcg acgccatgcc
      301 cgcccttcac cgtcagcgcc tgcccgacga atttctgcca gccgcagtcg agccccgcga
      361 atttgcccgc accgcgcgca tcgatgacat gcttcgccga gagccgtccg ccgcgcgcca
      421 gcagcagatg gtcgggcgcg acatgcttcg ccttgtcggc gatgatgtgc ccctcgggca
      481 gcgccgccgc gaccgcttcg gcaagcgcct cgccggtgat gctcttatac cccatgcgca
      541 gcctgcgctc gtgcgacgga aagcggacgt cgtagcgcgg ccagtgatgg cgcacgagcg
      601 gcgcgacgag ccagcggtcc ttcttcgcga tgtcgctgtc gaaaaaggac cagatatgat
      661 tgccgccgac cggccccggt tcgatcagcc ggacgtcgag atcgggccgc ttcgccgcca
      721 gcgcaagcac cgcgagcccg ccggcgagcc cgccgccgac gatcgcgatg tcgcatttat
      781 cgatccgctg ccccgccatc cctccgcctt agccacgcga gcgcgtcggc gcaaagccat
      841 tgcaacggtg ccgcgagcga ctagccttgc gcgatgaccg gctggacgat ccttttttcc
      901 gcgctcgcga tgaccgcgat cgtcggcgtg cgctatctga tcaccagcgg cgccttcgcc
      961 ttcgcgacga agtggaagca ccccggcctc tatcgcgggc tggaaccgca gatgaaacgc
     1021 gagatcggct ggagcctcgc gagcgccgcg atatacggcg tcccggcggg catcgtcgcc
     1081 tggggctggc aggaacatgg ctggacgcgc atctataccg acatcgacgc ctttccgctc
     1141 tggtacctgc ccgtcagcct cttcgcctat ctcctgctcc acgacacatg gttctattgg
     1201 acgcaccgct ggatgcaccg cccggcgctg ttccgcgccg cgcatgcggt tcatcatgcg
     1261 agtcgtccgc cgaccgcctg ggcggcgatg agcttccatc cgctggaggc gataaccggc
     1321 gcggtcgtca tccccgcgct cgtctttctg gttcccatcc atgtcggggt gctcggcctc
     1381 gtgctcgcga tcatgacggt gatgggggtc ggcaatcata tggggtggga gatgtttccg
     1441 cgcgcgcttg ttcatggacc cgcagggcaa tggctgataa ccgcgacgca tcatcaggcg
     1501 catcacgcgg cttaccgggg gaattatggt ctctatttcc gcttttggga caaggcgtgc
     1561 ggcacggata tcgggcttgg ctcttttgcc gatgctgctg gccgccgcga gcccgccgcc
     1621 gccgacggtt gaggtcagcg tcaccggtct gcgcagcgcg aaggggcaga tcctcgtctg
     1681 cctgaccacg aaccgcaagg cctttcccga ttgcagcaag gatgcgggct cggtgcgcat
     1741 ggcggtgaag gcggccgagg cggaaaattt cgccgttcac gcgcccgccg ccgggaccta
     1801 tgcgatcgcg gtcgtgcatg acgagaacgg caacaacaaa ctcgacaagg cgatctttct
     1861 gccgaaggaa ggcttcggct tttcgcgcaa cccgacgatc accgtcgggc ccccgagctt
     1921 caaatcggca agcttcgcgg tcgcgggcga catgcgccag tcgatcagga tgaaatatat
     1981 gctgtagggg agggcggtgc gatggtcggg cctcgtcggc ggtgagcgtc ggctttgggg
     2041 tggggtgccg acagtccgct tttacaccac gaagcagaaa ggctgccgtg ccctgctaga
     2101 gcgcgttgcg cttggagatc attgatcagc caaagaggcg acccatgctt tcttttcttt
     2161 gcaggcatcc agcctcggcg tgaaggtcgg ccggtcttag ccgacaagcc aaccccattt
     2221 cctccagtag ccagacgacg cggaagccgc gcccttcacc caagtccgtg agcgacatgg
     2281 ctcaaatcct ttcaggaaga cgcgccatca acaagacgac gcggcaaatc gcgccacaaa
     2341 tcgggttcga acatcctgag attgactccc atgcgctggt aattgggatc agttggcgtc
     2401 cagtgtgtcg tacagccaca ggcttggcag tgccacg
//
LOCUS       DATMSU010000256         5619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_14054, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000256 DATMSU010000000
VERSION     DATMSU010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14054"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VN034_06855"
     CDS             <1..727
                     /locus_tag="VN034_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV60354.1"
                     /translation="LSSAVTNRNRAVIRSPVSGVVLARQVEPGQTVAASFNTPTLFII
                     AEDLSAMQLRVEIDEADVGQVREGQKANFTVDAYPGRRFPAVVERVDQASSNTASQAS
                     QQAAATAATGNAVVSYEARLAVANADGLLRPGMTATATIATGSTAKQLLVPNSALRFE
                     PDAKEKEAASVLNPEIGLEQTEQQATIGVGSRQRVWVLQADGTLKPVDVVTGRSDGRL
                     TVVTSKALRRGMKVVTGKGAEGK"
     gene            724..1455
                     /locus_tag="VN034_06860"
     CDS             724..1455
                     /locus_tag="VN034_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV60355.1"
                     /translation="MTPVPSRDPPLIELEGITKTFGKGEAAFQALKGVDMRIERGDFV
                     AVMGPSGSGKSTTMNILGCLDVPTSGTFRFKGIEVQALDRDQRSLLRRRYLGFVFQGF
                     NLLARTTAVENVELPLLYRGEKKSVRHAAAMNALDQVGLAPWADHTPAELSGGQQQRV
                     AIARALVTQPDVLLADEPTGNLDTERSIEIMQLLTRLNGEGITVLMVTHEEEMAGFAH
                     TIVRFRDGLVETVDRGARAGTEEAR"
     gene            1455..2654
                     /locus_tag="VN034_06865"
     CDS             1455..2654
                     /locus_tag="VN034_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV60356.1"
                     /translation="MFGATLLLAFREIRRHLLRSFLTTLGIIIGVAAVITMVTLGNGV
                     TASIKEQISSLGSNVLIVFPVQGDRGTPRPFKQADVEAVSKQIAGVEDVSGSVSSSAT
                     AFHNGQSWRTSVQGVENAFLRAQSIEIELGRNFTREEESAGKNVCLIGPKVRKAIFAA
                     GSSPLGASIRLNNVSCTVIGVLKERGQGGGPDQDSDNVAMVPLKMAQRRFTGSDDLHY
                     FVIKYDSAYASSTIQNSLVDLLRERRLLQGTADNDFNIVDTAQVNQALGAATGALTAM
                     VAVIAGISLLVGGIGIMNIMLVSVTERTREIGIRLAIGALAREVQLQFLTEAVVLCCF
                     GGIVGIALAFILSWGMATVIDVPFIFNPVVNALSFLFSALMGITFGYYPARRASKLDP
                     IDALRHE"
     gene            2891..5554
                     /locus_tag="VN034_06870"
     CDS             2891..5554
                     /locus_tag="VN034_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60357.1"
                     /translation="MTLRNILLGATMLCAATAPAYAFAQDAAAPADAGTAVDDSDYGN
                     DIIVTASKRSQTLQDTPVAVSVTSAAQIEDAQIRDLIDLQTAVPSLRVSQLQSSANTN
                     FIIRGFGNGANNAGIEPSVGVFIDGVYRSRSAAQIGDLPNVERIEVLRGPQSTLFGKN
                     ASAGVISIVTQKPQYEFGGSAEITYGNYDAITVKGDITGPISDTVAFSLGGSYNRRDG
                     YAQDLNLDTDVNDRNRWGVRGQLLFEPTDALSIRLIGDYDKIDENCCIAGNVIAGPTV
                     AITDLLAGGTSVDRENPFSYDVYNNFLSTNEIENYGGSAQIDYDLGNLALTSITAYRQ
                     VRADTNQDSDFTAADLIGEKSDYVDIDTFTQEIRLTSDFDGPINFLLGGYYFNEKVNQ
                     QSAIKFGDDFRPYADALIRASTANALNVNSLEAILGAANGADYTGDFFRAGDGLDEAY
                     KMKNEAFSIFGTVDFEVTDRLTLTLGGNYTKDRKRFSTNVVSSDTFSGIDLRGSGGRV
                     IAGQALATGVGQALGLPAGTLATPDQIQAFAGAQPAIFGQINAQATAFGQLNANLTTA
                     QAAADGNPLTVGNPFLALTPFQFLPPFLNVPNAVESGKTNDGDFSYSVRAAYELTDTV
                     NIYATYATGFKASSINLSRDSRPTAEDLPAIISGGLGLPNLVSGSRFANPEESTVYEF
                     GLKGQWDVAAVNVAVFKQSIKGFQSNVFTGTGFALANAGKQSTFGIEFDGSVRPVDGL
                     NLTLAATYLDAKYDSFVNSAFGDISGTTPAGIPELSLSMGGTYTHEFAGGTKAIAHLD
                     YSYESPVQIVEGLSGFPASVAQNLKREVNSLNASFTIRLTNGIDVGVWGRNLTNAQYL
                     TTIFPAVAQAGSVSGYPSQPRTYGGTIRYKF"
BASE COUNT         1011 a         1846 c         1807 g          955 t
ORIGIN      
        1 gctttcgtcg gcggtgacca accgcaaccg cgcggtgatc cgctcgcccg tgtcgggggt
       61 cgtgctcgca cgccaggtcg agccggggca gaccgtcgcg gcgagcttca acacgccgac
      121 cctgttcatc atcgccgagg atctgtccgc gatgcagctg cgcgtcgaga tcgacgaagc
      181 cgacgtcggg caagtgcgcg aaggacagaa agcgaatttc accgtcgatg cctatcccgg
      241 ccgccgtttt cccgcggtcg tcgagcgcgt cgatcaggcg tcgagcaaca ccgcctcgca
      301 ggcgagccag caggcggcgg caaccgccgc gaccggcaat gcggtggtca gctatgaagc
      361 gcggctcgcg gtcgcgaacg ccgacgggct gctccggccc ggcatgaccg cgaccgcgac
      421 gatcgcgacg ggaagcaccg cgaagcagct tctcgttccc aatagcgcgc tgcgtttcga
      481 gcccgacgcg aaggagaagg aggcggcgag cgtgctcaat cccgagatcg ggctcgagca
      541 gaccgagcag caggcgacga tcggcgtcgg gagccgccag cgcgtctggg tgctccaggc
      601 cgacggcacg ctcaagccgg tcgacgtcgt caccgggcgg agcgacgggc gcctgaccgt
      661 cgtcacctcc aaggcgctgc ggcggggaat gaaggtggtg acgggcaagg gggcggaggg
      721 caagtgacgc ccgttccctc ccgcgatccc ccgctgatcg agctcgaagg catcaccaag
      781 accttcggca agggcgaggc ggcgttccag gcgctgaagg gcgtcgacat gcggatcgag
      841 cgcggcgatt tcgtcgcggt gatgggcccg tcgggatcgg gcaagtcgac gacgatgaac
      901 attctcggtt gccttgacgt gccgacgagc ggcaccttcc gcttcaaggg gatagaggtg
      961 caggcgctgg accgcgacca gcgcagcctg ctccggcggc gctacctcgg tttcgtcttt
     1021 cagggattca atctgctcgc gcgaacgact gcggtcgaga atgtcgagct tcccttgctc
     1081 tatcgcggcg agaagaagag cgtccggcac gccgcggcga tgaacgcgct cgatcaggtc
     1141 ggcctcgcgc cctgggccga ccatacgccc gccgagcttt caggcggcca gcaacagcgc
     1201 gtcgcgatcg cacgcgcgct ggtgacccag cccgacgtcc tgctcgccga cgagccgacg
     1261 ggcaatctcg acaccgaacg ctcgatcgag atcatgcagc ttctgacccg gctcaacggc
     1321 gagggcatca cggtattgat ggtgacgcac gaggaggaga tggcgggttt tgcgcacacc
     1381 atcgtccgct ttcgcgacgg cctggtcgaa acggtcgacc gcggcgcgcg cgccggcacc
     1441 gaagaggcgc gctgatgttc ggcgcgacgc tgctgctcgc ctttcgcgaa atccggcggc
     1501 acctgctgcg gtccttcctg acgacgctgg gcatcatcat cggcgtcgcc gcggtgatca
     1561 cgatggtgac gctgggcaac ggcgtcaccg cgtcgatcaa ggagcagatt tcgtcgctcg
     1621 gatcgaacgt gctcatcgtc tttccggtgc agggcgatcg cgggacgccg cggccgttca
     1681 agcaggccga tgtcgaagcg gtgagcaaac agatcgccgg ggtggaggat gtgtcgggca
     1741 gcgtttcgtc gagcgcgacc gcttttcaca acgggcagag ctggcggacg agcgtgcagg
     1801 gggtcgaaaa cgccttcctt cgcgcccagt cgatcgagat cgagctcggc cgcaacttca
     1861 cccgcgaaga ggaaagcgcg gggaagaatg tctgcctgat cggtccgaag gtgcgcaagg
     1921 cgatctttgc cgcgggcagc agcccgctcg gcgcgagcat ccgcctgaac aatgtctcgt
     1981 gcaccgtgat cggcgtcctc aaggagcgcg ggcagggggg cggacccgat caggacagcg
     2041 acaatgtcgc gatggtgccg ctcaagatgg cacagcggcg cttcaccggc agcgacgacc
     2101 tgcattattt cgtgatcaaa tatgacagcg cctatgcgag cagcacgatc cagaattcgc
     2161 tcgtcgatct gttgcgcgag cggcggttgc tgcagggcac ggccgacaat gatttcaaca
     2221 tcgtcgacac cgcgcaggtc aatcaggcgc tcggcgccgc gacgggcgcg ctgaccgcga
     2281 tggtcgcggt gatcgcgggg atcagcctgc tggtcggcgg gatcggcatc atgaacatca
     2341 tgctggtgtc ggtcactgag cggacgcgcg agatcggcat ccgcctcgcg atcggggcgc
     2401 tcgcgcgcga ggtgcagctc cagtttctga ccgaggcggt ggtgctctgc tgcttcggcg
     2461 gcatcgtcgg catcgcgctc gccttcatcc tgtcgtgggg gatggccacg gtgatcgacg
     2521 tgcccttcat cttcaacccg gtcgtcaacg cgctgagctt tctcttctcg gcgctgatgg
     2581 gcatcacctt cggctattat ccggcgcgcc gggcgtcgaa gctcgatccg atcgacgccc
     2641 ttcggcacga atgatggcaa ggccggtttg cggccgtgcg tcagctattt ccgcggttgc
     2701 aaatagccgg attacgcctt ccgtagcgta aactgttgct tttgtgccac taatggacgg
     2761 aaaagccgga cggtcacgcg cgcgcgcgtt gacgccgcag agcggtgcgt tagacccgcc
     2821 gttcactgcc gggcatctcc cgataagcct ttggctgcgc ttcggtggtt ttggagagag
     2881 agacatcatc atgactttgc gcaacatcct gttgggcgcc accatgctgt gcgccgccac
     2941 cgcccccgct tatgctttcg cccaggatgc cgccgcgccg gcggatgccg ggacggccgt
     3001 cgacgatagt gattatggca acgacatcat cgtcaccgcg agcaagcggt cgcagacgct
     3061 gcaggacacg ccggtcgccg tttcggtgac gtcggcggcg cagattgaag acgcgcagat
     3121 ccgcgacctc atcgacctcc agaccgcggt gcccagcctg cgcgtctcgc agctccagtc
     3181 gagcgcgaac accaacttca tcatccgcgg tttcggcaac ggcgcgaaca atgcgggcat
     3241 cgagccctcg gtcggcgtct tcatcgacgg cgtctatcgc tcgcgctcgg ccgcgcagat
     3301 cggcgacctt ccgaacgtcg agcgcatcga ggtgctgcgc ggcccgcagt cgacgctgtt
     3361 cggcaagaat gcctcggcgg gcgtgatcag catcgtcacg caaaagccgc aatatgaatt
     3421 cggcggttcg gccgaaatca cctatggcaa ttatgacgcg atcaccgtca agggcgacat
     3481 caccggcccg atcagcgaca ccgtcgcctt ctcgctcggc ggcagctaca accgccgcga
     3541 cggctatgcg caagacctca acctcgacac cgacgtcaac gaccgcaacc gctggggcgt
     3601 gcgcggccag ctcctgttcg aaccgaccga cgcgctgtcg atccgcctga tcggcgacta
     3661 tgacaagatc gacgagaatt gctgcatcgc gggcaatgtt atcgccggcc cgacggtggc
     3721 gatcaccgac ctgctcgccg gcggcaccag cgtcgaccgc gaaaatccct tctcctacga
     3781 cgtctacaat aatttcctgt cgacgaacga gatcgagaat tacggcggtt cggcgcagat
     3841 cgactatgac ctcggcaatc tcgcgctgac ctcgatcacc gcctatcgcc aggtgcgtgc
     3901 cgacaccaac caggattcgg acttcaccgc cgccgacctg atcggcgaga agagcgacta
     3961 tgtcgacatc gacaccttca cgcaggaaat ccgcctgacc tcggacttcg acggcccgat
     4021 caacttcctg ctcggcggct attatttcaa cgaaaaggtc aaccagcaga gcgcgatcaa
     4081 gttcggcgac gatttccgtc cctatgccga cgcgctgatc cgcgcatcga cggcgaatgc
     4141 gctgaacgtc aactcgctcg aggctattct cggcgcggcg aacggggccg attatacggg
     4201 cgacttcttc cgcgccggcg acgggctcga cgaagcctat aagatgaaga acgaggcctt
     4261 ttcgattttc ggcaccgtcg atttcgaggt caccgatcgc ctgacgctga cgctgggcgg
     4321 caattatacg aaggaccgca agcgcttctc gacgaatgtc gtcagcagcg acaccttctc
     4381 gggtatcgac ctgcgcggca gcggcggccg cgtcattgcg ggacaggcgc ttgcaaccgg
     4441 tgtcggtcag gcgcttggcc ttccggccgg cacgcttgcg acccccgacc agatccaggc
     4501 attcgcgggc gcccagccgg cgatcttcgg tcagatcaac gcgcaggcca ccgcctttgg
     4561 tcagctcaac gccaatctga cgacggcgca ggccgctgcc gatggcaatc cgttgacggt
     4621 cggcaacccg ttcctcgcgc tgacgccgtt ccagttcctg ccgcccttcc tcaacgttcc
     4681 gaacgcggtc gaaagcggca agacgaacga cggcgacttc tcgtacagtg tgcgcgccgc
     4741 ctatgaactc accgacacgg tcaatatcta tgcgacctat gcgacgggct tcaaggcgag
     4801 ctcgatcaac ctgtcgcgtg acagccgtcc gaccgcggaa gacctgccgg cgatcatctc
     4861 gggcggcctc ggtcttccga acctggtttc gggctcgcgc ttcgcgaatc ccgaggaatc
     4921 gaccgtctat gaattcggtc tgaagggtca atgggacgtc gccgcggtga acgtcgcggt
     4981 gttcaagcag tcgatcaagg gtttccagtc gaacgtcttc accggtaccg gcttcgcgct
     5041 cgccaacgcg ggcaagcagt cgaccttcgg tatcgagttc gacggatcgg tgcgtccggt
     5101 cgacgggctc aacctgacgc tggcggcgac ctatctcgac gcgaagtacg acagcttcgt
     5161 caattcggcg ttcggcgata tctcgggcac cacgcccgcc ggcattcccg aactgtcgct
     5221 gtcgatgggc ggcacctata cgcacgagtt cgcgggcggg accaaggcga tcgcgcacct
     5281 cgactacagc tatgagagcc cggtccagat cgtcgaaggc ctgtcgggct tccccgcgag
     5341 cgtcgcgcag aatctgaagc gcgaagtgaa ctcgctcaac gcgtcgttca cgatccgcct
     5401 gaccaacggc atcgacgtag gcgtgtgggg ccgcaacctc accaacgcgc aatatctgac
     5461 gacgatcttc ccggcggtcg cgcaggcggg cagcgtctct ggctatccga gccagccgcg
     5521 cacctatggc ggaaccatcc gctacaaatt ctgatcgcgc ggcgccgggc ggtcggcgcc
     5581 cggcccccgc ggcagcggga caaaagaaac cccggcctc
//
LOCUS       DATMSU010000257        14105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_99392, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000257 DATMSU010000000
VERSION     DATMSU010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99392"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1490)
                     /locus_tag="VN034_06875"
     CDS             complement(144..1490)
                     /locus_tag="VN034_06875"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWV60358.1"
                     /translation="MSVNLEQWISDHNIDEVECIVPDINGVQRGKVLPAKKFLSSVKD
                     KSLRIPGSVFICTIDGHYPEDIDDIWDKDPDKVLIADPDTICVAPGFTSPTAFVIADA
                     FNRDGTEVDIAPRTILKKVLGLYEEKGWKPIIAPEVEFYLVSQNTDPDFPLTPPTGQS
                     GRSENASQPYGLEAMNEYEDIIDHIYDDCELMGLDIDTMIHEMGAAQLEVNFIHGDPL
                     RLADEVFLFKRAVRNVAKQHDVYATFMANPMAGQPGSAMHIHQSIVDAETGRNLFATA
                     NGRDSAAFRSYIAGLIRFMPQISPMWAPNVNSFRRMRPDSAAPINVQWGEDNRSCGFR
                     VPIADKNNRRVENRLPGADSNPYLAIAASLVCGYIGMVDRMVPPKPITGSAYNRARTL
                     PRTLEAALDRFASCKKVRNLLGDDFFEIFFAVKDYELFNYQSVVSSWEREHLLMRV"
     gene            1643..2959
                     /locus_tag="VN034_06880"
     CDS             1643..2959
                     /locus_tag="VN034_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWV60359.1"
                     /translation="MRGDNDQLASFWMPSTANRAFKSQPRQLVSASGMFYRSSDGREI
                     LDGTSGLWCSNAGHCRPEISEAIAKTAATLDFAPTFQLGHPLPFELAQRLAELMPGGL
                     DRIFFTNSGSESVDTALKIALAIQRAKGEGTRTRLIGRERGYHGTGFGGISVGGLVNN
                     RRAFGGGLPGVDHLRHTHDIERNAFTRDFPQHGAELADDLQRLVDLHGAETIAAVIVE
                     PMAGSTGVLVPPVGYLQRLREICDRHGIILIFDEVITAFGRVGGATASEAWGVTPDII
                     TMAKGLTNAAVPMGAVAVKRELHDAVIDSVAGGIELFHGYTYSGHPLASAAGLATLDL
                     YARDGLFDRANELASYWEDAAHGLKERRHVIDIRTIGLVAGIELEPRPGAPTARAMEL
                     FHACFDNGLLVRATGDIIALSPPLIVEKSQIDEMFGRIADLLAAID"
     gene            complement(2956..4176)
                     /locus_tag="VN034_06885"
     CDS             complement(2956..4176)
                     /locus_tag="VN034_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV60360.1"
                     /translation="MFDRLSSLVIALTLVVIAAIFAALTGGDLLAIAMMAVAGFAAAG
                     TVYSALPAPLPEAPSAVPAAEAPPVSLLRHPDFARWVDQEKDPLLGVSDNIVAIANDA
                     AIRLLGRHIVGADIRTAIRHPAAAEWLSRISDGPLETVNLVDFPRPGQRWTMRVAALS
                     GGQRIVMLSDHSAIDAADRMRSDFVANASHELRTPLAAILGYVETLQDMNGDADAPTR
                     SRFLSIIHREAGRMQQLVIDLLSISRVEADRFRRPTTPVDLAAIVQTSVAQLRDSEQP
                     RAKDIVATLGEAPQPMLGDGAQLGQLAHNIISNAMKYGHPGTPVTVELAREGSRVRLS
                     VSDEGDGIAPDHLPRLTERFYRVDEARSRSVGGTGLGLAIVKHISERHQGQLDIESEL
                     GKGTRVSVTFPLAA"
     gene            4326..4739
                     /locus_tag="VN034_06890"
     CDS             4326..4739
                     /locus_tag="VN034_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60361.1"
                     /translation="MENSHIPAGQPEMDGEAVPTRRRMLALGAVGVSAALTIRPAFAQ
                     TAVSVMNCEIPVPGPTAAGQYIDRAGKLVPAGTRGAFPGSPRPFTGEEVKRAFRGQTL
                     PGTTYNQSQAYLQYIRRLQAGQSGFTCFASITTSR"
     gene            4799..5800
                     /locus_tag="VN034_06895"
     CDS             4799..5800
                     /locus_tag="VN034_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWV60362.1"
                     /translation="MKLASLKKGRDGRLVVVSDDLAWYADAGQIAATMQAALDNWAYA
                     APRLTALAEDLNHDAIPKERFHERDAASPLPRAYQWADGSAYVNHVALVRQARGAEMP
                     DSFWHDPLMYQGGSDAFLAPRDPIPLGDPAWGCDMEAEVVVVTGDVPAGIDAVAARDK
                     ILLVGLTNDVSLRGLIPAELAKGFGFFQSKPSSAMSPVFVTPEALGDRWKDGKLHGTL
                     SVDLNGEPLGRADAGVDMTFDFGALIAHAAKTRDLGAGTIIGSGTVSNRDADGGPGKP
                     ISEGGLGYSCLAEVRTVETIKLGEPKTPFMRKGDTVRIWMDDERHHSIFGAIEQSVG"
     gene            complement(5858..6904)
                     /locus_tag="VN034_06900"
     CDS             complement(5858..6904)
                     /locus_tag="VN034_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWV60363.1"
                     /translation="MNSSLLFSSAFTGAQSGPTLLGIDVVWAGTMLAAVGVFAVLFAI
                     YNALTVRNPMTKRVKALNDRREQLKAGITASTAKRRARLVRKNQTADKMRSFLGRLQV
                     LQDSQIKEVQQKLAQAGIRSKDLAVAVIFGRMVLPILFGGLAIILIYWVDMWPDLTSF
                     KRSASTMVALILGYKAPDLFVDNKRQKRTNAIRKGLPDALDLLVICAEAGLTVDSAFA
                     RVAKELGRAYPELGEEFALTSIELSFLTERRQAFENFAYRVNLESVKGVVTTMIQTEK
                     YGTPLASALRVLSAEFRNERMMRAEEKAARLPAIMTVPLILFILPVLFIVILGPAACS
                     LSDAMSGGTFGGKG"
     gene            complement(6919..7887)
                     /locus_tag="VN034_06905"
     CDS             complement(6919..7887)
                     /locus_tag="VN034_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWV60364.1"
                     /translation="MNLPVILIIAGAFLAFALLVILVGGPSAGKAKTRRLAMVKDRHA
                     ASTEAVVAAQMRKTIQSGRADDSSLTHLLPRREELEKRIRRTGKSWTLTQYMFATMIV
                     FVVTTGAMLIVRAPVLMAGLVGLLLSLALPYMVVGMTIKRRVAAFNNRFPDAIDLLVR
                     GLKSGLPVTETFQVVSHELPGPVGDEFKGVVERIRIGNTMEAALQESAEMLGTPEFQF
                     FCITIQIQRETGGNLAETLANLSDVLRKRAQMKLKIRAMSSEAKASAYIVGALPFFVF
                     GVVWMVNPSYLAGFFFEQRLIVAGIGGLIWMSIGAAIMAKMVNFEI"
     gene            complement(7945..9216)
                     /locus_tag="VN034_06910"
     CDS             complement(7945..9216)
                     /locus_tag="VN034_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HWV60365.1"
                     /translation="MNAPFKSAGLRDPFNAFVCDDDTLELIRAAAGDMGWPIEKCNKG
                     GLRNAVQSLSVSASPNILFVDMSESGDPINDINALAEVCEPGTVVIAAGQVNDVRLYR
                     DLLSSGIQDYLLKPLSLEQVRESLTMAQAMLSAPKHSDMHDDKPHHMMGIVGVRGGVG
                     ASLVATSLAWAISEQADRQTALLDLDVHFGTGALTLDLEPGRGLIDAIDNPSRIDGLF
                     IERAMVRASDKLSLLSAEAPIHQPVMTDGSAFFQLEEELRNAFEMTIVDIPRQVLIPF
                     PHLVSEMGTILLVSDVTLAAARDTIRLLSWFKQNLPGARVVLVANKFQSAIGELSRKE
                     FESSIERPIDIVIPFDPKLVAQAAKLGKSYAETCKGTKSAQVWNNLMRLVLDGADVDA
                     EDAPVAIAKGKSGGSLLGKLGLAKKGAKQAA"
     gene            complement(9213..9854)
                     /locus_tag="VN034_06915"
     CDS             complement(9213..9854)
                     /locus_tag="VN034_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HWV60366.1"
                     /translation="MKKIATWTVLACAISLGACTGAAYSNRSLESVHQPVVRNNLYQF
                     DVAIANGELPPSEQGRLQGWLDAMGVRYGDRIAVEDPSAYGAASAQATVRAMVERRGL
                     LLSREVPVTTGAVPEGHMRVVVTRASASVPGCPNWDSKSSLNPANATSSNYGCATNSN
                     IAAMVADPNDLIKGTRDTGHDPVAATRAIQTYRTKPQTGAGELAKPSTSGGSQ"
     gene            complement(9880..11361)
                     /locus_tag="VN034_06920"
     CDS             complement(9880..11361)
                     /locus_tag="VN034_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HWV60367.1"
                     /translation="MNSKANFRAAALARTLAIGLVAATLVAAPSQPAIAQAVQNASSS
                     LELSVGRGRLVSLPATMSDVFVANNEVADVQVRSGRQLYVFGKKPGETSIYATDSSGR
                     VVFSTIARVGNNIETIDQMLSLAMPEASIVPTPMNGFVLLTGTVQSPDDAAEAERLVQ
                     AFIGEETKVLSRLRTATPLQVNLQVRIAEVNRSLVKEISGNLLTRDTEGPLGNGFLGG
                     IFGGRQAGTITTDANGNTVYQITTPAGTRSLAGAGRLFGLDLIASLDIGERSGLVATL
                     AQPNLTAISGETADFLAGGEFPVPIPGNFAGTTIEYRKYGVSLAYTPTVLSNGRISLR
                     VRPEVSELSTEGAIEMQGFQVPALTIRRAETTVELGSGESFMIAGLLNNRSIGAIDKM
                     PGLGDVPILGTLFKSDSFRRGETELVIVVTPYLVEPVSANDIKLPTDAFQDTHDLQRL
                     LLNQTSDGVTGGDRPKPRLDTNVGDADRPRNGGGNAAPGFNIK"
     gene            complement(11363..12403)
                     /gene="cpaB"
                     /locus_tag="VN034_06925"
     CDS             complement(11363..12403)
                     /gene="cpaB"
                     /locus_tag="VN034_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HWV60368.1"
                     /translation="MDTRKIMLIVGALVIAIGAAFGVNKMMRGAAAPSARAAAAPEIT
                     GPTILVATRQLPVGTIIGPDSFRFQPWPKELVEQAYFLKDKTDVNTLVGTVVRHAITA
                     GQPLTQGALVHPDDRGFLAAALGPGMRAVTVKVSREEGVGGFVFPGDRVDVVLAQQIS
                     VDSETSSYPDKELFTAETIIRNVRVLATDQRYNAEDETGKTPVRTFSSVTLEATPEIA
                     EKIAVAQDMGKLSLALRPLAESAGELEAAIASGEINVPAGADSAAEKKMLAQAAARPT
                     ASRSSATTGGDVSRFWVPVRGRVTSPRPQQSEGRGEYAPSSAGRQAAVSTGPVVRVTR
                     GDKMTEVPVGGK"
     gene            complement(12458..12940)
                     /locus_tag="VN034_06930"
     CDS             complement(12458..12940)
                     /locus_tag="VN034_06930"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HWV60369.1"
                     /translation="MDSGAISLGLMALLGLLMIAAAISDVRSRTISNQLNAAMALLAI
                     PFWIASGLALWPDIPIQFGAAFLVFLLFAGLFAIGAMGGGDVKMIGAVMLWIPLPLFL
                     STLTIMAIGGGILSALMLIHMKLRPSDKPVEVPYGVAIAAAGLWALHQHYLNQFQAIG
                     "
     gene            13039..>14105
                     /locus_tag="VN034_06935"
     CDS             13039..>14105
                     /locus_tag="VN034_06935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV60370.1"
                     /translation="MTDRPPSATDVLIVGAGIAGASLAAALAPWRRVMLVEAEDVPGY
                     HATGRSAAFWHETYGGAHVQPLTLASLETLLSPDPAFSDHAFLSPRSALTIGQASEAA
                     AVDAFVAEFQAKGVDVERMSGAAVAARIPGLRSEWSEAAYEAACRDIDVGGLHAAYLR
                     AAKRAGALLINRAPLRSARRVGDGWRVELGEGEIDAAVIVNAAGAWADKVAAACGVAP
                     LGIQPYRRTVLQVRLGVAVPAELPLVIHVGGQFYFKGESAGRVWLSPHDETPSTAHDV
                     APEDMDVATAIERMQSVVDWPVAAVERKWAGLRSFAPDRIPVFGPDPHEGRFVWCAGQ
                     GGFGIQTAPAIAGLLAAQLGAP"
BASE COUNT         2414 a         4451 c         4715 g         2525 t
ORIGIN      
        1 accccatctc ctccttccgc atccccgagc gaagacgagg ggccaggccg agcgaagtcg
       61 aggcccgatg cgaattctcg cttcgctcga acgagcccct cgtcttcgct cggggatacg
      121 gaccttgaaa gacactatcc ggatcagacc cgcatcagca gatgttcgcg ctcccagctc
      181 gacaccaccg actgatagtt gaacagctca taatccttca ccgcgaagaa gatttcgaag
      241 aaatcgtcgc cgagcaggtt gcggaccttc ttgcacgagg cgaagcggtc gagcgcggct
      301 tccagggtgc gcggcagcgt gcgtgcgcgg ttataggcac tgccggtaat cggcttgggc
      361 gggaccatgc ggtcgaccat gccgatatag ccgcacacca gcgacgccgc gatcgccaga
      421 taggggttgc tgtcggcgcc gggcaggcga ttttcgacgc ggcggttgtt cttgtcggcg
      481 atcggcacgc gaaagccgca gctccgattg tcctcgcccc actggacgtt gatcggcgcg
      541 gcgctgtcgg ggcgcatacg gcgaaagctg ttcacattgg gcgcccacat cggcgagatt
      601 tgcggcatga agcggatgag gccggctatg tagctgcgga acgccgcgct gtcgcggccg
      661 ttggcggtcg cgaacaggtt gcgcccggtc tcggcatcga cgatcgactg gtggatgtgc
      721 attgcgctgc ccggctggcc cgccatcggg ttcgccatga aggtggcgta gacatcgtgc
      781 tgctttgcca cattgcgcac ggcgcgcttg aacaggaaca cctcgtcggc gaggcgcagc
      841 gggtcgccgt ggatgaaatt cacctcgagc tgcgcggcgc ccatttcatg gatcatcgtg
      901 tcgatatcga gccccatcag ctcgcaatcg tcatagatat gatcgatgat atcctcatat
      961 tcgttcatcg cttccagtcc gtagggctgg ctggcgttct cgctacgccc cgactggccc
     1021 gtcggcgggg tcagcggaaa atcggggtcg gtgttctgcg aaacgagata aaattcgacc
     1081 tcgggcgcga tgatcggttt ccagcccttt tcttcataaa gacccagcac tttcttgagg
     1141 atcgtgcgcg gtgcgatgtc gacttcggtg ccgtcgcggt tgaacgcgtc ggcgatcacg
     1201 aatgcggtcg gcgaggtgaa gcccggggcg acgcagatcg tgtcggggtc ggcgatcagg
     1261 accttgtcgg gatccttgtc ccagatatcg tcgatgtctt cgggataatg gccgtcgatc
     1321 gtgcagatga agacgctgcc cggaatgcgc agcgacttgt ccttgaccga ggagaggaac
     1381 ttcttggccg gcaatacctt gccgcgctgg accccgttga tgtcggggac gatacattcg
     1441 acttcgtcga tattatggtc gctgatccat tgttccagat tgactgacat gcgcccccga
     1501 ttgggccgag gagccggccc gtttgtggaa gaccagccta tcgtgaagtt ggggtcagcg
     1561 ccagagggcg gcccgttttg gcattgccag ccgcgcgggt gcccgtcaga ttgacgatga
     1621 atatcacagg agcgggttcg cgatgagagg tgacaacgac cagctggcgt ccttctggat
     1681 gccctctacc gcgaaccgcg cgttcaagag ccagccgcgc cagctcgtct cggcaagcgg
     1741 catgttctat cggtcgagcg acgggcgcga gatcctcgat ggcacgtcgg gcctgtggtg
     1801 cagcaacgcc ggccattgcc gccccgagat cagcgaagcg atcgcaaaga cggccgcgac
     1861 gctcgacttc gcgccgacgt ttcagctcgg ccacccgctg ccgttcgaac tggcgcagcg
     1921 gcttgccgag ctgatgcccg ggggactcga ccggatattc tttacgaaca gcggctccga
     1981 atcggttgat acggcgttga aaatcgcgct cgctatccaa cgcgcgaagg gcgaggggac
     2041 gcggacgcgg ctgatcgggc gcgagcgcgg ctatcacggc accggttttg gcgggatcag
     2101 cgtcggcggg ctcgtcaaca accgccgcgc gttcggcggc gggctgcccg gggtcgatca
     2161 tctgcggcat acgcacgaca tcgaacgcaa cgcctttacc cgggactttc cgcagcatgg
     2221 cgccgagctg gccgacgatc tccagcgcct cgtcgacctg cacggtgccg aaacgatcgc
     2281 cgcggtgatc gtcgagccga tggcgggatc gacgggggtg cttgtgccgc ccgtcggcta
     2341 tctccagcgc ctgcgcgaga tttgcgaccg gcacggcatc atcctgatct tcgatgaggt
     2401 gatcaccgcc tttggccgcg tcggcggcgc gacggcgtcg gaagcctggg gcgtcacgcc
     2461 cgacatcatc accatggcaa agggcctcac caacgccgcg gtgccgatgg gggcggtcgc
     2521 ggtgaagcgc gaattgcatg atgcggtgat cgacagcgta gcgggcggga tcgagctgtt
     2581 ccacggctat acctattcgg ggcatccgct ggcgagcgcg gcggggctcg cgacgctcga
     2641 tctctatgcg cgcgatggcc tgttcgaccg cgcgaacgaa ctggcgagct attgggagga
     2701 tgccgcgcac ggcctgaaag agcggcgtca cgtcatcgat attcgcacca tcgggctcgt
     2761 cgcggggatc gaactcgagc cgcgccccgg cgcgccgacc gcgcgcgcga tggaattgtt
     2821 tcacgcctgt ttcgacaatg ggctgctcgt ccgcgcgacg ggcgacatca tcgcgctgtc
     2881 gccgccgctg atcgttgaaa agtcccagat cgacgaaatg ttcggacgga tcgccgatct
     2941 gctggccgcg atcgactagg cggcgagcgg gaaggtcacc gacacacgcg tgcccttgcc
     3001 aagctcgctc tcgatgtcga gctggccctg gtggcgttcg ctgatatgct tgacgatcgc
     3061 gaggccgagg ccggtgccgc cgaccgagcg gctgcgcgct tcgtcgacgc ggtagaagcg
     3121 ttcggtcagg cgcggcagat gatcgggcgc gatgccgtcg ccttcgtcgc tgaccgatag
     3181 ccgcacgcgg cttccctcgc gcgcgagttc gaccgtgacg ggggttcccg ggtggccata
     3241 tttcatcgcg ttggagatga tattgtgcgc gagctgcccc agttgcgcac cgtcgccgag
     3301 catcggctgc ggcgcctcgc ccagcgtcgc gacgatgtcc ttcgcgcgcg gctgttcgct
     3361 gtcgcgaagc tgcgcgaccg acgtctggac gatcgccgcg agatcgaccg gcgtcgtcgg
     3421 gcgacgaaaa cggtcggcct cgacccgcga gatcgagagc aggtcgatga ccagctgttg
     3481 catccgcccc gcctcgcggt ggatgatcga caggaagcgg ctgcgcgtcg gcgcgtcggc
     3541 gtcgccgttc atatcctgca gcgtttcgac atagccgagg atcgccgcga gcggcgtgcg
     3601 caactcgtgg ctcgcattgg cgacgaaatc cgatcgcatc cggtcggcgg cgtcgatcgc
     3661 cgaatggtcc gacagcatga cgatgcgctg tccgcccgac agcgcggcga cgcgcatcgt
     3721 ccagcgctgg ccgggccgcg ggaaatcgac gaggttgacc gtttcgagcg ggccgtcgct
     3781 gatacgcgac agccactcgg ccgccgccgg atggcggatc gcggtgcgta tgtcagcccc
     3841 gacgatatgg cgcccgagca gccggatcgc ggcgtcgttg gcgatcgcga cgatattgtc
     3901 cgaaacgccg aggagcgggt ccttttcctg atcgacccag cgggcgaaat cggggtggcg
     3961 gagaagcgaa accggcgggg cttcagccgc cggcaccgcg ctcggcgctt cgggaagcgg
     4021 ggcgggaagg gcgctgtaaa ccgtgcctgc ggcggcgaag cccgcgaccg ccatcatcgc
     4081 gatcgccagg agatcgcccc ccgtcagcgc ggcaaaaatc gccgcgatga cgaccagcgt
     4141 cagggcgatg acgaggctgg acagacgatc gaacatgcgc cgcccgcgtc gcacgaagcg
     4201 ggggatcagc gcaagcggct ttgcgggtgt taacgctttc gggaaggctg tgccaaagcg
     4261 ggaagcggag aggctgcgac ggtcttttgc ttttcctagg cgcttcattg cgttatgcgc
     4321 cggccatgga aaacagccac attcccgccg gccagcccga aatggatggc gaagccgttc
     4381 cgacgcgccg caggatgctg gcgctcggcg ccgtcggcgt gtcggccgcg ctgacgatcc
     4441 ggcccgcttt cgcgcagacc gccgtgtcgg tgatgaactg cgagattccc gttcccggac
     4501 cgacggcggc ggggcaatat atcgatcgcg cgggcaagct ggttccggcg ggaacgaggg
     4561 gcgcttttcc gggatcgccg cggccgttca ccggcgagga agtgaagcgc gcgttccgcg
     4621 gccagacgct gcccggcacg acctacaacc agtcgcaggc ctatcttcaa tatatccggc
     4681 gcttgcaggc ggggcagagc ggctttacct gtttcgcatc gatcacgacg tcgcgctgat
     4741 tatcgcttcc tgacaggcga tgacggtttc agccgcatcg tcagacaagg gagttttcgt
     4801 gaaactagca tcgctcaaaa agggccgcga cggccgtctg gtcgtcgttt cggacgatct
     4861 cgcctggtat gccgacgccg gccagatcgc ggcgacgatg caggcggcgc tcgacaattg
     4921 ggcctatgcc gcgccgcgcc tcaccgcgct cgccgaagac ctcaaccatg acgcgattcc
     4981 gaaagagcgg ttccacgagc gcgatgcggc gtcgccgctg ccgcgcgcct atcaatgggc
     5041 cgacggcagc gcctatgtga accatgtcgc gctcgtccgg caggcgcgcg gcgccgaaat
     5101 gcccgacagc ttctggcacg acccgctgat gtatcagggc ggcagcgacg cctttctggc
     5161 cccccgcgat ccgatcccgc tcggcgatcc cgcatggggc tgcgacatgg aggccgaggt
     5221 ggtcgtcgtc accggcgacg tgccggcggg aatcgatgcc gtcgcggcgc gcgacaagat
     5281 cctgctcgtc ggcctcacca acgacgtatc gctgcgtggg ctgatcccgg ccgaactcgc
     5341 caagggcttc ggctttttcc aatcgaaacc gtcaagcgcg atgtcgccgg tgttcgtgac
     5401 gcccgaggcg ctcggcgatc gctggaagga cggcaagctg cacggcacgc tgtcggtcga
     5461 cctgaacggt gagccgctgg gccgcgccga tgccggggtc gacatgactt tcgatttcgg
     5521 cgcgctgatc gcccacgctg cgaagacgcg cgatctgggt gcgggcacga tcatcgggtc
     5581 gggcaccgtg tcgaaccgcg atgccgacgg cggcccgggc aagccgatca gcgagggcgg
     5641 gctgggttat agctgcctcg ccgaagtacg gacggtcgag acgatcaagc tgggcgagcc
     5701 caagacgccg ttcatgcgaa agggcgatac ggtccgcatc tggatggacg acgagcgcca
     5761 ccacagcatt ttcggcgcga tcgaacagag cgtaggctga cagcaaagag ggcggaatcg
     5821 cttccgccct cttttcttta accggtgcga gctgtcgtca gcccttgccg ccgaaggtgc
     5881 cgcccgacat cgcatcgctc agcgaacagg ccgccgggcc cagaatgacg atgaacagca
     5941 cgggcaggat gaacaggatc agcggcaccg tcatgatcgc gggcagacgc gcggcctttt
     6001 cctcggcgcg catcatgcgt tcgttacgga attcggctga cagcacacga agcgcggacg
     6061 cgagtggcgt gccgtatttt tcggtctgga tcatggtcgt gaccacgccc ttcaccgatt
     6121 cgagattcac acgataggcg aaattctcga atgcctggcg gcgctcggtc aggaagctga
     6181 gttcgatcga cgtcagggcg aactcctcgc ccagttcggg ataggcgcgg cccagttctt
     6241 tcgccacgcg ggcaaaagcg gagtcgaccg tcaagcccgc ctcggcgcaa atgacgagga
     6301 ggtcgagcgc atcgggaagg cctttgcgga tcgcgttggt ccgcttctgg cgcttattgt
     6361 cgacgaaaag gtcgggggcc ttatagccga ggatcagcgc gaccatggta gaggcggagc
     6421 gtttgaacga cgtcaggtcg ggccacatgt cgacccagta gatcaagatg atggcaagcc
     6481 caccgaacag gatcggcagg accatgcggc cgaagatcac cgcgacggcg agatccttcg
     6541 accggatacc agcctgcgcg agcttttgct ggacttcctt gatctgacta tcctgaagga
     6601 cctgaagcct gccgaggaag gaccgcatct tgtccgcggt ctggttcttg cggaccagcc
     6661 gcgcgcggcg cttggccgtc gaggcggtga tgccggcctt cagctgttcg cggcggtcgt
     6721 tgagcgcctt gacgcgcttc gtcatcggat tgcgcacggt cagcgcattg tagatcgcga
     6781 acaataccgc gaagacgccg accgcggcca gcatggtgcc ggcccatacg acgtcgatgc
     6841 cgagcagggt cggaccgctt tgcgcgcctg tgaaagcgga cgagaaaagg aggctgctgt
     6901 tcatcgtttc tgcccccctt aaatttcgaa attgaccatc ttggccatga tggcggcgcc
     6961 gatgctcatc cagatcaacc cgccgatgcc ggcgacgatc aatcgctgct cgaagaaaaa
     7021 gcctgcaaga tagctagggt tcaccatcca cacgacgccg aacacgaaga agggcagtgc
     7081 accgacgata taggcggatg ccttggcttc cgacgacatt gcgcggatct tgagcttcat
     7141 ctgcgcgcgc ttgcgcagca cgtccgacag gttggccagc gtttcggcca gattgccgcc
     7201 ggtttcgcgc tggatctgga tcgtgatgca gaagaactga aattcggggg tgccgagcat
     7261 ttcggcggat tcctgcagcg cggcttccat agtgttgccg atgcggatgc gttcgacgac
     7321 tcccttgaat tcgtcgccca ccgggccggg aagctcgtgg ctcacgacct gaaaggtttc
     7381 ggtgaccggc aggcccgatt tgaggccgcg tacgagcagg tcgatcgcgt cgggaaaacg
     7441 gttgttgaac gctgcgacgc gccttttgat cgtcatcccg acgaccatat agggcagtgc
     7501 gaggctgagc agcaacccga cgagcccggc catcaggact ggggcgcgga cgatgagcat
     7561 cgcgccggtg gtgacgacga acacgatcat cgtcgcaaac atatattggg tcagcgtcca
     7621 gctcttgccg gtccggcgga tccttttctc gagctcttcg cggcgcggca gcagatgggt
     7681 cagcgacgag tcgtcggcgc ggcccgactg gatcgtcttg cgcatctgcg ccgcgaccac
     7741 cgcttcggtc gaagccgcgt gacgatcctt gaccatcgcg agccgccgcg tcttcgcttt
     7801 gcccgccgaa ggtcctccga ccaggatcac gaggagcgcg aaggccagaa aggccccggc
     7861 aatgataagg atgactggca ggttcatggt cagctagtgt ccgcttctgt cactgaggat
     7921 cgcgacggcg cgtcaggcgc gccctcacgc cgcctgtttc gcgccttttt tggccaagcc
     7981 gagcttgccg agcaacgagc cgcccgactt gccttttgcg atagcgaccg gcgcatcctc
     8041 ggcatcgaca tcggcgccgt cgaggacaag gcgcatcaga ttgttccaga cctgcgccga
     8101 cttggtgcct ttgcaggttt cggcgtagga ttttccgagc ttcgccgctt gggcgaccag
     8161 cttgggatcg aacggaatga cgatgtcgat cggtcgttcg atcgacgatt caaactcctt
     8221 gcgcgacagt tcgccgatcg cgctctggaa cttgttggcg acgagcacca cccgcgcgcc
     8281 cggcaggttc tgcttgaacc acgacagaag gcggatcgtg tcgcgcgccg cggcgagcgt
     8341 tacgtcgctg acgagcagga tcgttcccat ttccgacacg agatgcggga aggggatcag
     8401 gacctggcgc gggatgtcga caatcgtcat ctcgaacgcg ttgcgcagct cttcctccag
     8461 ctggaagaac gccgagccgt cggtcattac cggctgatgg ataggggcct cggccgacaa
     8521 caggctgagc ttgtccgacg cgcgcaccat cgcgcgctcg atgaacaggc cgtcgatacg
     8581 gcttggattg tcgatcgcat cgatcaggcc gcgcccgggt tccaggtcga gcgtgagcgc
     8641 ccccgttccg aaatggacgt cgagatcgag caacgccgtc tggcggtctg cctgttcgct
     8701 gatcgcccag gcaagcgacg ttgcaacgag cgaggctccg acgccgccgc gaacaccgac
     8761 gatgcccatc atgtggtgcg gtttgtcgtc gtgcatgtcc gaatgtttcg gagccgatag
     8821 catagcctgg gccatggtca gcgattcgcg gacctgttcg agcgacagcg gcttcagaag
     8881 atagtcctga atgccgctcg acagcaggtc gcggtacagg cgcacgtcgt tgacctggcc
     8941 cgccgctatc acgaccgttc cgggttcgca gacttccgcc agcgcattga tatcgttgat
     9001 cggatcaccc gattcggaca tgtcgacgaa caggatgttc ggactggccg acaccgacag
     9061 cgactgcacg gcgttgcgca ggccgccctt gttgcacttc tcgatcggcc agcccatgtc
     9121 gcccgccgcc gcacggatca actccagcgt gtcgtcgtcg cagacgaagg cgttgaaggg
     9181 atcacgaagc cctgctgatt tgaaaggagc gttcactggc tgcctccgct ggtggatggt
     9241 ttggccaatt cgccggcacc cgtctggggt ttcgtgcgat aggtctggat cgcgcgcgtc
     9301 gccgcgaccg gatcatgccc ggtatcgcgc gtgcccttga tgaggtcgtt gggatcggcg
     9361 accatcgcgg cgatgttgct gttggtcgcg cagccatagt tggacgaggt cgcattggcc
     9421 gggttcagcg aggatttgct gtcccaattg gggcatccgg ggaccgaagc cgacgcgcgc
     9481 gtcacgacga cgcgcatatg gccttcggga accgcacccg tcgttaccgg cacctcgcgg
     9541 ctcagtagca gcccgcggcg ctcgaccatc gctctgacgg tcgcctgcgc cgacgctgcg
     9601 ccatatgccg acggatcctc gacggcgatg cggtcgccgt agcgaacgcc catcgcgtcg
     9661 agccagcctt gcaggcggcc ctgttcgctg ggcggcagtt cgccattcgc gatcgcgacg
     9721 tcgaattgat agaggttgtt gcgcactacc ggctgatgca ccgattccag gctgcggttg
     9781 ctgtatgcgg ccccggtgca ggccccgagc gagatcgcgc atgccaggac agtccaagtt
     9841 gcgattttct tcatcacttt gctcctgaaa tccagcgcgt cacttgatgt tgaaacccgg
     9901 cgcggcattg cctccgccgt tccgcgggcg gtcggcgtcg ccgacattgg tatcgagccg
     9961 cggtttgggg cggtcgcctc cggtcacgcc gtcgctcgtc tggttgagta gcaaccgctg
    10021 cagatcgtgc gtgtcctgga aggcgtcggt cggcagcttg atgtcattcg ccgacaccgg
    10081 ctcgaccaga tagggcgtca cgacgatgac gagttcggtt tcgccgcgac ggaagctgtc
    10141 cgacttgaaa agcgtgccga gtatcggcac atcgcccagg ccgggcatct tgtcgatcgc
    10201 gccgatcgag cgattgttca acaaccctgc gatcatgaag ctttcgcccg agccgagttc
    10261 gaccgtcgtt tcggcgcggc ggatcgtcag cgccggaacc tggaagccct gcatctcgat
    10321 cgcgccttcg gtcgacagtt cggatacctc cgggcggacg cgcaggctga tccggccgtt
    10381 cgacagcacg gtcggggtat aggcgaggct gacgccatat ttgcgatatt cgatcgtcgt
    10441 ccccgcgaag ttgccgggga tcggcaccgg gaattcaccg cccgccagga agtcggcggt
    10501 ctcgcccgaa atggcggtca gattgggctg cgccagcgtg gcgaccaggc cggaccgttc
    10561 gccgatatcg agcgaggcga tcaagtcgag cccgaacagg cggcccgcac cggcgaggct
    10621 gcgcgttccg gcgggggtgg tgatctggta gacggtgttg ccgttggcat cggtcgtgat
    10681 cgtgcccgcc tgacggcccc cgaaaatccc gcccagaaag ccattgccga gcggaccttc
    10741 ggtatcgcgg gtcaggaggt tgccgctgat ttccttgacg agcgagcggt tcacttcggc
    10801 gatacgaacc tgcagattga cctgcagcgg cgtggcggta cgcaggcgcg agagcacctt
    10861 ggtttcctcg ccgataaatg cctgaaccag ccgttcggcc tcggcggcgt cgtcgggcga
    10921 ttgaaccgtg ccggtgagca gcacaaagcc gttcatcggg gtcggcacaa tgctggcctc
    10981 cggcatcgcg agcgacagca tctggtcgat cgtctcgata ttgttgccga cgcgagcgat
    11041 ggtggagaag accacccggc cgctggaatc ggtggcatag atgctggttt cgccgggctt
    11101 tttgccaaag acatagagct ggcggcccga gcggacctgg acgtccgcga cctcgttgtt
    11161 ggcgacgaag acatcggaca tggtcgccgg aaggctgacc aggcggccgc gccccaccga
    11221 cagttcgagg ctgctactgg cgttctggac cgcctgggcg atagccggct gcgaaggcgc
    11281 cgcgaccagc gtcgctgcca ccaggccgat ggccagggtg cgcgccagag cggccgccct
    11341 gaaattggct ttgctgttca tcttacttac ccccaaccgg aacttcggtc atcttgtcgc
    11401 cgcgggtgac gcgcacgacc ggaccggtcg acacggcagc ctggcgaccc gcgctggacg
    11461 gcgcatactc cccccggcct tcagactgct gcgggcgcgg cgaggtgacg cgtccgcgca
    11521 ccggaaccca gaaacgcgac acgtcgccgc cggtcgtcgc cgacgagcgg ctcgcggtcg
    11581 gacgcgcggc ggcttgcgcc agcatcttct tttcggcggc gctgtcggcc cctgctggaa
    11641 cgttgatttc gcccgaggcg atcgcggcct caagttcgcc ggcgctttct gccagcggac
    11701 gcagcgcgag cgacaatttg cccatgtcct gcgcgacggc gatcttctcg gcgatctccg
    11761 gcgttgcttc gagcgtcacc gagctgaagg tgcgcaccgg cgtcttgccc gtctcgtcct
    11821 cggcattgta gcgctggtcg gtcgcgagaa cgcgaacgtt gcggatgatc gtctcggcag
    11881 tgaacaattc cttgtccggg taggacgacg tctcggaatc gaccgagatc tgctgtgcga
    11941 gtacgacgtc gacacggtcg cccggaaaga cgaaaccgcc gacgccctct tcgcgcgaaa
    12001 ccttgacggt gaccgcgcgc attccagggc caagcgccgc ggcaaggaaa ccgcggtcgt
    12061 cgggatgaac cagggcgccc tgcgtgagcg gctgtcccgc ggtgatcgcg tgacgcacga
    12121 cggtgccgac cagcgtattc acgtcggtct tgtctttcag gaaataggcc tgttcgacca
    12181 attccttcgg ccacggctgg aaccggaagc tgtcaggacc gataatcgtg ccgaccggca
    12241 gctgccgcgt ggcaaccagg atcgtcgggc cggtaatttc cggcgccgcg gcggctcttg
    12301 ccgacggagc ggctgcgccg cgcatcatct tgttgacccc gaacgccgcg ccgatggcaa
    12361 tcaccagcgc gccgacgatc agcattatct ttcgcgtatc catgacgagt tttcgccctc
    12421 ctgcacccac cagagacggt cgtgcttttc cctcaggtca tccgatagcc tgaaactggt
    12481 taagatagtg ttggtgaagc gcccacaggc ccgcagccgc gatagcaacc ccgtagggca
    12541 cctcgaccgg cttgtccgaa ggtcgcaatt tcatatggat aagcatcagg gcggacagga
    12601 taccgccgcc gatggccatg atcgtcagcg tcgacaggaa caggggaagc gggatccaca
    12661 gcatcaccgc gccgatcatt ttcacgtcgc cgccgcccat cgccccgatc gcgaacaagc
    12721 cggcgaagag caggaaaacc agaaaggccg ctccgaactg gatcgggata tcgggccaca
    12781 gcgcgaggcc ggaagcgatc cagaagggga tcgcgagcag cgccatcgcg gcgttgagct
    12841 ggttggaaat cgttcgcgac cggacgtcgc tgatcgccgc ggcaatcatc aacaggccga
    12901 gaagggccat caggccgagc gagattgcgc cgctatccat cggacccttc ctacgcaaca
    12961 tggcttacca aagagtaacc atgttcgggt tgacgccgcg gcttgcattc cgcggtccag
    13021 cggatatgcg gcgggaccat gaccgaccgg ccgccctccg ccaccgatgt gctgattgtc
    13081 ggcgccggta tcgccggcgc gagcctggcg gctgcgctcg cgccctggcg ccgcgtcatg
    13141 ctggtcgagg ccgaggacgt gcccggctat cacgctacgg gtcggtccgc ggccttctgg
    13201 cacgagacct atggcggggc gcacgtccag cccttgacgc tggcctcgct ggagacgttg
    13261 ctaagccccg atccggcctt ttcggatcat gccttcctgt cgccgcgctc ggcgttgacg
    13321 atcgggcagg catccgaagc ggcggcggtc gacgcgttcg tcgccgagtt ccaggcgaag
    13381 ggcgtcgatg tcgagcgcat gtcgggcgcc gcggttgccg caagaattcc ggggctgcgt
    13441 tccgaatgga gcgaagcggc ctatgaggcc gcgtgcaggg atatcgacgt tggcggcctc
    13501 cacgcggcct atcttcgcgc ggcaaagcgc gcgggcgccc tcctgatcaa ccgtgcgccg
    13561 ctgcgcagtg cccgccgcgt cggcgacgga tggagggtag aacttggcga gggggagata
    13621 gacgcggcgg tgatcgtcaa tgccgcgggc gcttgggcgg acaaggtcgc ggccgcttgc
    13681 ggcgtcgcgc cgctcgggat tcagccatat cggcggacgg tcctgcaggt tcgcctcggt
    13741 gtcgcggttc cggccgaact gccgctggtg atccatgtcg gtggccaatt ctatttcaag
    13801 ggcgagagcg cgggacgggt gtggctgagc ccccacgacg agaccccgag cacggcgcat
    13861 gacgtggcgc ccgaagatat ggacgtcgcc accgcgatcg agcggatgca atcggtcgtc
    13921 gactggccgg tcgccgccgt cgagcgcaaa tgggcgggtc tgcgcagctt cgcgcccgac
    13981 cggattcccg ttttcggccc cgacccccat gaaggccggt tcgtctggtg cgcggggcag
    14041 ggcgggtttg gcatccagac cgcaccggcg attgccgggc tgctcgcggc gcaattgggc
    14101 gcgcc
//
LOCUS       DATMSU010000258         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_70847, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000258 DATMSU010000000
VERSION     DATMSU010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70847"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VN034_06940"
     CDS             <1..500
                     /locus_tag="VN034_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWV60371.1"
                     /translation="IAWRGPMAGKALEQLVDASWGDIDTLIVDLPPGTGDVQLTMIQR
                     HKPAGAVIVSTPQDLALMDATRAISLFEQADVPIIGLVENMAGYACPHCGEVSDPFGS
                     GGAEAAAGTMGLDFLGRVPLSMGIRLASDGGVPPAAGTDPAGEPFHAIAAKVADWLNA
                     RKKGS"
     gene            502..924
                     /locus_tag="VN034_06945"
     CDS             502..924
                     /locus_tag="VN034_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWV60372.1"
                     /translation="MAINDEGEIRELLGQPRRIAVVGASPNPARPSNGVLAFLMRQGH
                     DVIAVNPGHAGSTIHGAPVVATLANVEPPAEIVDIFRNSEDAGAAVDEAIVHGAKAVW
                     MQMGVINEAAAQRAEAAGLVVVMDRCPKVEVPRLGLLK"
     gene            939..1517
                     /locus_tag="VN034_06950"
     CDS             939..1517
                     /locus_tag="VN034_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60373.1"
                     /translation="MFAASALAGCATTPAATNPQDAFFAALSARCGKAYAGKLASDQD
                     ADADMRGKAMVMHIRHCTNDRIEIPFHIDGLGPNGGWDRSRTWIVSRTAAGLRLKHDH
                     RHADGTPDAVTLYGGDTADAGTAARQTFPVDAESIAMFEGTGRSVSATNIWSIETTPA
                     GFTYGLNREGRHFRVAFDYARPVTPPPTPWGW"
     gene            1572..>2069
                     /locus_tag="VN034_06955"
     CDS             1572..>2069
                     /locus_tag="VN034_06955"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWV60374.1"
                     /translation="MRTQTFMLIPLLALGACSEKAPDPRGVEHDETLLSVSATGRSET
                     RPDEARFTAGVSTIAASAEDATRRNNETMTKVTEALKAFGVAGKDLQTRQLTVNRIEW
                     GANKGKFEAVNQVEVRMRAVDKAGEAVAATTEAGANVLSGPTLRVSDQEAATKSAYAA
                     AYRAAR"
BASE COUNT          359 a          714 c          716 g          280 t
ORIGIN      
        1 cgatcgcgtg gcgcggcccg atggcgggca aggcgctcga acagctcgtc gatgcgagct
       61 ggggcgacat cgacacgctg atcgtcgacc tgccgccggg caccggcgac gtccagctga
      121 cgatgatcca gcggcacaag cccgcgggcg cggtgatcgt ctcgaccccg caggacctcg
      181 cgctgatgga tgcgacgcgc gcgatcagcc tgttcgaaca ggccgacgtg ccgatcatcg
      241 ggctggtcga gaatatggcg ggctacgcct gcccgcattg cggcgaggtc agcgacccat
      301 tcggcagcgg cggcgcagaa gcggccgcgg ggacgatggg gctcgacttc ctcggccgcg
      361 tgccgctgtc gatgggcatc cgcctcgcga gcgacggcgg cgtccctccg gcggcgggaa
      421 ccgatcccgc gggtgaacca ttccacgcga tagcggcgaa ggtcgccgac tggctgaacg
      481 cgcgcaagaa gggaagctga catggcgatc aacgacgaag gcgaaatccg cgaactgctc
      541 ggacagccgc gccgcatcgc ggtggtcggc gcctcgccca acccggcacg cccgtcgaac
      601 ggcgtgctcg cctttctgat gcgccaaggc catgatgtga tcgcggtcaa tcccggccac
      661 gccgggtcga cgatccacgg cgcccccgtc gtcgcgacgc tcgccaatgt cgagccgccg
      721 gccgagatcg tcgacatctt tcgcaacagc gaagacgcgg gcgccgcggt cgacgaggcg
      781 atcgtccacg gcgcgaaggc ggtgtggatg cagatgggcg tcatcaacga agccgccgcg
      841 cagcgcgccg aagccgcggg gctggtggtc gtgatggacc gctgccccaa ggtcgaggtg
      901 ccgcgcctcg gcctgctgaa gtgaaggctg ctctttcgtt gttcgccgcg agcgcgctcg
      961 caggctgcgc gacgacgcct gccgccacga acccgcaaga cgctttcttc gccgcgctct
     1021 cggcgcgctg cgggaaagcc tatgccggca agctcgcgag cgatcaggac gccgacgccg
     1081 acatgcgcgg caaggcgatg gtgatgcaca tccgccattg cacgaacgac cgcatcgaaa
     1141 tcccctttca catcgacggg ctcggcccga acggcggctg ggatcgctcg cgcacctgga
     1201 tcgtcagccg cacagcggcg ggccttcgcc tcaagcatga tcatcgtcac gccgacggca
     1261 cgcccgacgc ggtgacgctt tatggcggcg acaccgccga tgccggaacc gcggcccgcc
     1321 agaccttccc ggtcgacgcc gaatcgatcg cgatgttcga gggcaccggc cgcagcgttt
     1381 cggcgaccaa catctggtcg atcgaaacca cccccgcggg ctttacctat gggctgaacc
     1441 gcgaaggaag gcatttccgc gtcgcgttcg actatgccag acccgtgacg ccgccaccga
     1501 cgccgtgggg ctggtaaggg gaacccttcc gcccgtcatg cgctagcccc gcaccggacg
     1561 ggagaaaata tatgcgcacc cagaccttta tgctgatccc cctgctcgcg ctcggcgcgt
     1621 gcagcgaaaa ggcgcccgat ccgcgcggcg tcgagcatga cgaaaccttg ctgtcggtat
     1681 cggcgaccgg ccggtccgaa acgcggcccg acgaggcgcg cttcaccgcc ggggtcagca
     1741 cgatcgccgc atcggccgag gatgcgacgc ggcggaacaa tgaaacgatg accaaggtga
     1801 ccgaggcgct caaggccttc ggggtcgcgg gcaaggacct ccagacgcgc cagctgacgg
     1861 tgaaccggat cgaatggggc gcgaacaagg gcaagttcga ggcggtcaat caggtcgagg
     1921 tccgcatgcg cgcggtcgac aaggcgggcg aggcggtcgc cgcgacgacc gaggccgggg
     1981 cgaatgtcct gtcggggccg acgctccgcg tctccgatca ggaggccgcg accaaatcgg
     2041 cctatgccgc ggcctatcgc gccgcgcgg
//
LOCUS       DATMSU010000259         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_146673,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000259 DATMSU010000000
VERSION     DATMSU010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146673"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..862
                     /locus_tag="VN034_06960"
     CDS             143..862
                     /locus_tag="VN034_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HWV60375.1"
                     /translation="MSSGKGGSAKGSGGRGGLHVRVKTAKKRSVSSTRWLQRQLNDPY
                     VRRAQAEGYRSRAAYKLIELDEKFGFLKKSRAVVDLGITPGGWSQVVRKANPRARVTG
                     IDLLACEPIEGVAILEMDFMDDAAPDALIEALGSAPDLVISDMAANTVGHPQTDHLRT
                     IGLAETAADFAVQNLQPGGTFVAKVFAGGADRELLTLLKRYFSTVKHAKPPASRKGSP
                     ELYVIAQGFKGRHDGSEADAE"
     gene            895..>2266
                     /locus_tag="VN034_06965"
     CDS             895..>2266
                     /locus_tag="VN034_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWV60376.1"
                     /translation="MGKSRRPVAALTLAALMLASCGGDGGTLGSGPVTVTPTPSPTPT
                     PTPNCALASRQAFAKAVIDEWYLFPGDVASANPGAYSDVQSYIDALVAPARALNKDRF
                     FTYITSIAEENAFYENGSSAGFGVLMAYDSVNQRIVIAEAYEGAPAFAAGIDRGTAIV
                     GIGTSSGNVRTVASIAAAEGIDGLTNALGPDDPGVSRVLRITDAAGTRDVTVAKADYA
                     LDPVSDRYGAKIISEGGRSYGYLNLRTFIASADDQLTAAFADFRAQGVTDIIVDFRYN
                     GGGLVSTANLMGDLMGAGRAGQLFSQTRFRPSKSAEEDDEHRFAPGAQSIAPTRIAFI
                     GTGSTASASELVINSMLPYLGADMTLVGSNTYGKPVGQIALDKAECDDRMRVVAFATS
                     NASGQSDYYDGLAPKIANSCAANDDLGFPLGDPREASIRAAIDFLAGNACTTRIADAS
                     ARTASRD"
BASE COUNT          407 a          752 c          753 g          354 t
ORIGIN      
        1 gcgctggctg caacgccagc tcaacgaccc ctatgtccgc cgcgcgcgtc ggcgttgccg
       61 accgcggcat ccgcgaaggc atcctccgca cgctggcgat gcagggccgc gacattcccg
      121 tcacccggcg cgagtatcgc aaatgagcag cgggaaaggc ggaagcgcca aaggatcggg
      181 ggggcgcggc gggctgcatg tgcgggtcaa gaccgcgaag aagcgcagcg tgtcgtcgac
      241 gcgctggctg caacggcaat tgaacgaccc ctatgtgcgc cgcgcgcagg ccgaaggcta
      301 tcgctcgcgc gccgcgtaca agctgatcga actcgacgag aaattcggtt tcctcaagaa
      361 atcgcgcgcg gtcgtcgacc tgggcatcac gccgggcggc tggtcgcagg tcgtgcgcaa
      421 ggcgaacccg cgcgcgcggg ttacgggaat cgacctgctt gcttgcgagc cgatcgaggg
      481 ggtcgcgatc ctcgaaatgg atttcatgga cgatgccgcg cccgatgcgc tgatcgaggc
      541 gctgggcagc gcccccgacc tcgtcatttc ggacatggcg gcgaacaccg tcggccatcc
      601 gcagaccgac catctgcgca cgatcggcct cgcggaaacc gccgccgatt tcgcggtgca
      661 gaatttgcag cccggcggga ctttcgtggc gaaagtgttc gcgggcggcg ccgaccgcga
      721 gttgttgact ttactaaagc gatacttttc aacggtaaag cacgccaagc cgcccgccag
      781 ccgcaaaggg tcgcccgaac tctatgttat cgcgcaaggg ttcaagggcc ggcacgatgg
      841 gagcgaagcg gacgccgaat aaaggtcgat gggtcgaacc gggggagaat attgatgggc
      901 aagtcgaggc ggccggtcgc ggcgctaaca ctcgcggctt tgatgctggc atcgtgcggc
      961 ggcgatggcg gaacgctggg cagcggcccg gttacggtca ccccgacgcc gagccctacc
     1021 ccgaccccga cgccgaattg cgcgctcgcc tcgcgccagg cctttgccaa ggcggtgatc
     1081 gacgaatggt atctgttccc gggcgacgtc gcgagcgcca accccggcgc ctatagcgac
     1141 gtccaatcct atatcgacgc gctggtcgcc ccggcgcgcg cgctgaacaa ggaccgcttc
     1201 ttcacctaca tcacctcgat cgccgaggag aatgcctttt acgaaaacgg atcgagcgcg
     1261 ggcttcggcg tgctcatggc ctatgattcg gtcaatcagc gtatcgtcat cgccgaagcc
     1321 tatgaaggcg cgccggcttt tgcggcgggg atcgaccgcg gcaccgcgat cgtcgggatc
     1381 ggcacgagca gcggtaatgt gcggacggtg gcaagcatcg ccgcggccga gggaatcgac
     1441 gggctcacca acgcacttgg cccggacgac cccggggtga gccgcgtgct gcgcatcacc
     1501 gacgcggcgg gcacgcgcga cgtcaccgtc gccaaggccg actatgcgct cgaccccgtt
     1561 tcggaccgct atggcgccaa gatcatcagc gagggcggcc gcagctatgg ctatctcaac
     1621 ctgcgcacct tcatcgcgtc ggcggacgat cagttgaccg ccgcctttgc cgatttccgc
     1681 gcgcagggcg tcaccgacat catcgtcgat ttccgctata atggcggcgg cctcgtttcg
     1741 acggccaatc tgatgggcga cctgatgggc gcgggacgcg cgggccagct tttttcgcag
     1801 acgcgtttcc ggccgagcaa atccgccgag gaggacgacg agcatcgctt cgcgccgggg
     1861 gcgcaatcga tcgcgccgac gcgcatcgcg ttcatcggca ccgggtcgac cgcctcggcg
     1921 agcgaactcg tgatcaattc gatgctgccc tatctcggcg ccgatatgac gcttgtcggc
     1981 agcaacacct atggcaagcc ggtcggccag atcgcgctcg acaaggccga atgcgacgac
     2041 cgtatgcgcg tcgtcgcatt cgccacgagc aatgcaagcg gtcagagcga ttattatgac
     2101 gggctggcgc cgaagatcgc gaacagctgc gccgcgaacg acgatctggg cttcccgctc
     2161 ggcgacccgc gcgaagcgtc gatccgcgcc gcgatcgact tcctcgccgg caacgcctgc
     2221 acgacgcgga tcgccgacgc gagcgcccgc acggcgtcgc gcgacc
//
LOCUS       DATMSU010000260         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_108897,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000260 DATMSU010000000
VERSION     DATMSU010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108897"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VN034_06970"
     CDS             <1..724
                     /locus_tag="VN034_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HWV60377.1"
                     /translation="QIARWREAGEGRPCDVMIDTGMNRLGLRVEEAAGGALNGLTIET
                     LLSHLASADEDSEQNARQLAAFRAIRQAIPARRYSLANSAGICLGTDYGFDLTRPGIA
                     LYGGTPRREAVGNIRQVVFPEARVLQVREVRQGETVGYGATWTAGRDSRIAVANIGYA
                     DGYLRCHAETGGARFDGQALPLVGRVSMDLVAFDATSIEGGAEGDWLALDYGLAATAA
                     RCGLSQYELLTGLGRRFERGWI"
     gene            849..1385
                     /gene="phaR"
                     /locus_tag="VN034_06975"
     CDS             849..1385
                     /gene="phaR"
                     /locus_tag="VN034_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HWV60378.1"
                     /translation="MAKSKGTAEDDVVTIKKYANRRLYDTERSCYITLDDLGTMVRDG
                     RDFRVVDAKSGEDITHNVLTQIIMDEETRGETLLPVNFLRHLIGMYGDKMQSMVPQYL
                     EASMTAFRKNQQDVRSAFEGALGANPLAELTRRNMEMFQQAAGAFIPGAAGSKAKDAE
                     IAALKAEIAELKAELAKK"
     gene            1429..2955
                     /gene="proS"
                     /locus_tag="VN034_06980"
     CDS             1429..2955
                     /gene="proS"
                     /locus_tag="VN034_06980"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWV60379.1"
                     /translation="MIKHALSVTRQADFAAWYQDVIAEADLAEESGVRGCMVIKPWGY
                     GIWERIQTVMDAAIKDAGVQNAYFPLFIPLSFFEKEADHVDGFAKEMAVVTHHRLIAD
                     GKGKLVPDPEAKLEEPLIVRPTSETVIGSAMSRWVQSWRDLPLKVNQWANVVRWEMRT
                     RMFLRTSEFLWQEGHTAHADKDDAMAETLRALEMYRAFAEDVLAMPVIAGEKPENERF
                     PGAVATYSIEAMMQDGKALQAGTSHYLGTGFAEAANIRYQDKDGGHSLCHTTSWGTST
                     RMIGGVIMTHGDDDGLRCPPRIAPHQIVIVPMLRDNDEDAAILDYCRDLETQLKALDA
                     FREPVRVLFDTGANKAQTKRWGWVKKGAPIIVEVGPRDVAGGNVAVIRRDRLYKDDGK
                     LNSAFIAKGDFVADAVATLEDIQSSLHGEARERLHGNIRRDVTDLKAHFAGDDKFVGW
                     AEVQWSRPSGATLDGIVEQLKALKLTMRNTPLDAAPVDGACFFTGEPAVERVLIGRTY
                     "
     gene            3027..3377
                     /locus_tag="VN034_06985"
     CDS             3027..3377
                     /locus_tag="VN034_06985"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWV60380.1"
                     /translation="MRYPLLVAAATLALAALPAAAHHGWSSYDETKPVTLTASFTELS
                     WGNPHGSAKMRWKGKLWDVILAPVGRMEARGLTRAEIEPGKRFRLTGYLRRDGTTEMR
                     VERVMIGKKTVELR"
     gene            3388..3873
                     /locus_tag="VN034_06990"
     CDS             3388..3873
                     /locus_tag="VN034_06990"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HWV60381.1"
                     /translation="MEPVLTVAAAWLDTVGVGPWSRGSALVYPVANTLHLLGLVMLVG
                     GIGVVDLRVIGLWRSLPMAELSRALTPVAIAGLVLMVVSGTVLFAADGTALARSAIFQ
                     RKLIIIAAALANAAAFRLIWRAHIAGWPDIAPGAARIMAAASLLLWLTAGASGRWIAY
                     S"
     gene            4083..>4571
                     /locus_tag="VN034_06995"
     CDS             4083..>4571
                     /locus_tag="VN034_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60382.1"
                     /translation="MKRQIAGSTMIAALLIGGITPAAAKPPSDVADLPGSRAAGAETA
                     MESRGYEMVKTSGGAQYWWNSGTKTCVSVVVSNGRYASVDKAGSSNCGHGGGGGDAAA
                     AVGLIAALASHKKKHGDHGDANHDSEYSRGYNDGLYGGQYDRDDSEGYHDGFMAGEAE
                     AAN"
BASE COUNT          839 a         1446 c         1552 g          734 t
ORIGIN      
        1 ccagatcgcg cgctggcgcg aggcgggcga ggggcgcccg tgcgacgtga tgatcgatac
       61 cgggatgaac cgcctcgggc ttcgcgtcga agaggcggcc ggcggcgcgc tcaacggctt
      121 gaccattgaa acgctgctca gccatctcgc ctcggccgat gaagacagcg aacagaatgc
      181 gcgccaattg gcggcgttcc gcgcgatccg gcaggcgata cccgcccggc gctacagcct
      241 cgcgaacagc gccggcatct gcctcggcac cgactatggc ttcgatctga cccgcccggg
      301 catcgcgctc tatggcggca cgccgcgccg cgaagccgtg ggaaatatcc ggcaggtggt
      361 attccccgag gcgcgcgtgc tgcaggtgcg ggaggtgcgc caaggcgaaa cggtcggcta
      421 tggcgcaacc tggaccgctg ggcgcgacag ccggatcgcg gtggccaata tcggctatgc
      481 cgatggctat ctgcgctgcc atgccgaaac aggcggcgcg cgctttgacg ggcaggcgct
      541 gccgctcgtc ggccgagtgt cgatggacct cgtggcattt gacgcgacca gcatcgaggg
      601 aggcgcggag ggtgactggc tggcgctcga ctatggtctt gcggcgacgg cggcgcgctg
      661 cggcctgtcc caatatgagc tgctcaccgg cttgggccgg aggttcgagc ggggctggat
      721 ataattgagc cgcgactcgc atcggcggcc acgcatgttg cgacgcatca taatttgatg
      781 ctaatgcagc agcctgtccg gtgcggcgcg gtcgccaggt tccgggagtg cagggagcgc
      841 gggtgaaaat ggcgaagtcc aaagggacag cggaagacga cgtcgtcacg atcaagaaat
      901 acgccaaccg gcggctctac gacaccgaac gcagttgcta catcacgctc gacgatctgg
      961 gcacgatggt gcgtgatggc cgcgattttc gcgtcgtcga tgccaaaagc ggcgaggaca
     1021 tcacccataa tgtccttacc cagatcatca tggacgagga aacgcgcggc gaaacgctgc
     1081 taccggtcaa tttcctgcgc catctgatcg gcatgtatgg cgacaagatg cagtcgatgg
     1141 ttccgcaata tctcgaagcc tcgatgacgg cgtttcgcaa gaaccagcag gacgtgcgtt
     1201 cggcgttcga gggggcgctc ggcgcgaacc cgctcgccga actcacccgc cgcaatatgg
     1261 aaatgttcca gcaagccgcg ggggccttca tcccaggcgc ggcgggcagc aaggcgaagg
     1321 atgccgagat cgccgcgctg aaggctgaaa tcgccgagct caaagccgag ctcgccaaga
     1381 agtaaccggg gccgctctcc ggcctcccaa gcatagaaaa caggacagat gatcaagcat
     1441 gcgctcagcg taacccgtca ggccgacttc gcggcctggt atcaggacgt cattgccgag
     1501 gccgatctcg ccgaggaatc aggggtgcgc ggctgcatgg tcatcaagcc gtggggctat
     1561 ggcatctggg aacgcatcca gacggtgatg gacgccgcga tcaaggatgc cggcgtccag
     1621 aacgcctatt tcccgctctt catcccgttg agcttctttg agaaggaagc cgaccatgtc
     1681 gatggttttg cgaaggaaat ggcggtcgtc acccatcacc gtctgatcgc ggacgggaag
     1741 ggcaagctcg tccccgatcc cgaggcgaaa ctcgaggagc cgctgattgt tcgcccgacg
     1801 tccgaaacgg tgatcggctc ggcgatgagc cgctgggtgc agagctggcg agacctgccg
     1861 ctgaaggtca accagtgggc caacgtcgtc cgctgggaaa tgcgcacgcg catgttcctg
     1921 cgcaccagcg aattcctgtg gcaggaaggc cataccgcgc atgccgacaa ggacgatgcg
     1981 atggccgaaa cgctgcgcgc gctcgaaatg taccgcgcct ttgccgagga tgtcctcgcg
     2041 atgccggtga tcgcgggcga aaagcccgag aatgagcggt ttccgggcgc cgtcgcgacc
     2101 tattcgatcg aggcgatgat gcaggacggc aaggcgctcc aggccggcac ctcgcactat
     2161 ctcggcaccg gtttcgccga ggccgcgaac atccgctatc aggacaagga cggcggccac
     2221 agcctctgcc acacgacgag ctggggcacc tcgacgcgca tgatcggcgg cgtcatcatg
     2281 acgcacggcg acgacgacgg gctacgctgt ccgccgcgca tcgcgccgca ccagatcgtc
     2341 atcgtgccga tgctccgcga caatgacgag gatgccgcga tcctcgatta ttgccgcgac
     2401 ctcgaaaccc agttgaaggc gctcgatgcc ttccgcgaac ccgtgcgcgt gctgttcgac
     2461 accggcgcga acaaggcgca gaccaagcgt tggggctggg tcaaaaaggg cgcaccgatc
     2521 atcgtcgagg tcggcccgcg cgacgtcgcc ggcggcaatg tcgcggtgat ccgtcgcgac
     2581 cggctctaca aggatgacgg caagctcaac tcggcgttca tcgcgaaagg cgatttcgtc
     2641 gccgacgcgg tggcgacgct cgaggatatc cagtcgagcc tccacggcga agcgcgcgag
     2701 cggctccatg gcaatatccg ccgcgacgtt accgatctga aggcgcattt tgccggcgac
     2761 gacaagttcg tcggctgggc cgaggtgcaa tggtcgcgcc cgagcggggc gacgctcgac
     2821 gggatcgtcg agcaattgaa ggcgctcaag ctgacgatgc gcaacacgcc gctcgacgcc
     2881 gccccggtcg acggcgcctg cttcttcacc ggcgaaccgg cggtcgagcg ggtgctgatc
     2941 gggcggacct actgagcggc gacgcccttg caccgggcag cctggagttc tagccttcgc
     3001 gccgaggggc tcgaaggaga ctgtcgatgc gctaccccct gctggtcgcg gccgcgacgc
     3061 tggccttggc cgcgctcccc gctgcggccc atcatggctg gtcgtcctat gacgagacca
     3121 agccggtcac cttgaccgcg tcgttcaccg aattgtcgtg gggcaacccg catggcagcg
     3181 cgaagatgcg ctggaagggc aagctctggg acgtcatcct ggcaccggtc ggccgcatgg
     3241 aggcgcgcgg gctgacgcgc gccgagatcg agcccggcaa acgctttcgc ctgaccggct
     3301 atctccggcg cgatggcacc accgaaatgc gggtggaacg cgtgatgatc ggcaagaaga
     3361 cggtcgaact gcgctgaggg gggcggcgtg gagcccgttc tgactgtcgc cgcggcgtgg
     3421 ctcgataccg tcggcgtcgg accctggtcg cgcggatcgg cgctggtcta tcccgtcgcc
     3481 aatacgctgc acttgctcgg cctcgtcatg ctggtcggcg gcatcggcgt cgtcgatttg
     3541 cgggtcatcg gcctgtggcg gagcctgccg atggcggaac tgtcgcgcgc gctgacgccc
     3601 gtcgccatcg ccgggctggt cctgatggtg gtcagcggca cggtcctctt cgccgccgac
     3661 ggcacggcgc tggcgcgatc agccattttc caacgaaaac tgatcatcat cgcagcggcg
     3721 ctggccaacg cggccgcctt tcggctgatc tggcgcgcgc atatcgcggg ctggcccgac
     3781 atcgcaccgg gagccgcgcg gatcatggcc gcggcttcgc tgcttctctg gctcaccgcg
     3841 ggcgcgtcgg ggcgctggat tgcctatagc tgacggcgcc ggcgcccgcg ctatcacccg
     3901 gtttgcccag ccgccgaccg atataagccc cggtcagccc gacgaaaatc gcgagcaaag
     3961 gggcagtgaa accggttcgg cccctgtcac atatctggta ggatgccgtc gctagacccg
     4021 ggcgatgcac ccggtctgcg gcggggcgca tcatgtttct ggcgttagac aggggaaagg
     4081 tcatgaagag acaaattgcc ggttcgacga tgattgccgc actgctgatc ggcggcataa
     4141 cgcccgccgc ggccaagccg ccttccgatg ttgccgacct tcccggttcg cgtgccgcgg
     4201 gcgccgaaac cgcgatggaa tcgcgcggct atgaaatggt gaaaaccagc ggcggcgcgc
     4261 aatattggtg gaacagcggg acgaagacct gcgtcagcgt cgtcgtttcg aacgggcgat
     4321 atgcgagcgt cgataaagcc ggttcgagca attgcggtca cggcggcgga ggcggcgatg
     4381 ccgcggcggc ggtcggcctg atcgcggcac ttgcgagcca caagaaaaag cacggcgatc
     4441 atggcgacgc caaccacgat agcgaatatt cgcgcggcta taatgacggc ctctatggcg
     4501 gccagtacga ccgcgacgac agcgagggct atcacgacgg cttcatggcc ggcgaagccg
     4561 aagccgccaa t
//
LOCUS       DATMSU010000261        12708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_175165,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000261 DATMSU010000000
VERSION     DATMSU010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 12708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12708
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175165"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1055
                     /locus_tag="VN034_07000"
     CDS             153..1055
                     /locus_tag="VN034_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60383.1"
                     /translation="MSVTLDGILRRHSRDIALVLGNGMNRHANAATLNSWDGLLMSIA
                     RDCIPGVNSVPPGTALTEFYDVVELKSPMGVGELQSQFCVSMKDWRPVAHHMQIAGWA
                     LRHDVPILTTNFDEVISQAVGGDFQFPKDPAFTAFYPWGCHFGPELVDDPCAGFAIWH
                     INGMARYKTSIRLGLTHYMGSVQRARGWLHGSGEDNLFRAKDRRDWRGARTWMHVVFN
                     KPLLFIGLGLTENEVFLRWLLIERAKYFRQFDGRKHPAWYVYVDDPKDERQAGKLFFL
                     ESVGVRCLQAPSYQTIYEADGWGG"
     gene            1064..2644
                     /locus_tag="VN034_07005"
     CDS             1064..2644
                     /locus_tag="VN034_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60384.1"
                     /translation="MTDDAEKAELDRDPAIWREGLDIQLGLADDEAFLRMVWGLNALQ
                     TGRADVGRPYFRNAPPEAAKDGIGGKHSVYPWELETLVNELLVTPKHAHGPLFDCRDW
                     SAIATLVNELRGLENAEYGQRRAERSILRELGRIGARQFEWQRGFFSIQQLYRSAYIY
                     GQKACNDHLVATTGLTMPVLTLVGFALMSSYLGSSAIQPGSDLALLESLGASRDELEK
                     AIALISRPLPDLRTEARQIRSDGELTAYKASVLRRFPCVLVGRGNSQMLAPLPELVSN
                     RLTSGLFYDVIGGGGPVRDETGRQFESYSYALLNAMLPEFQFEREWAYQTRLGQMKSP
                     DIMLALPDQTIGLAIECKALRMTVAARFGDEPQDERGYEEIVRGIFQLWRFFAHCRLG
                     LAGRDVTDEARGVILTLDDWFAARHSILEQLLKRAHARADEEKVPILPIDRRPIAFCA
                     ISEIEQTLRTATGASLLATIELAAGEKLGWIFPMVHQDLKAPKTEPKDFPFENEVGDL
                     LPWYRRIGEMADDGTFAD"
     gene            complement(2656..3420)
                     /locus_tag="VN034_07010"
     CDS             complement(2656..3420)
                     /locus_tag="VN034_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60385.1"
                     /translation="MKDAIRLNLVHGAAALAAAGRHVPLVRAIERAAAVGSIREDLRT
                     ALAALRLIENMQAEQERGEVKQLNDQTTITGALFTQAIILYARATISSGKRPSLLNER
                     GLSDEELATHNEVRLMRNSVIGHFGRGDDLEGGPVVREAVIVSFTRSQRQIGVYTVRA
                     QHKVILAQRLALLLERRLAQIAERQDAIFDSVQRLLSEALQSDRTLGPLLLRYPFDAD
                     AFCGNSDAARNLRAQLEAGELQDMDYVVSAVRPAAI"
     gene            3547..4023
                     /locus_tag="VN034_07015"
     CDS             3547..4023
                     /locus_tag="VN034_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60386.1"
                     /translation="MATELAALLLRNGKEHAESACLLYRAAHDIAHEENIEDEEAFAF
                     YGPYSLSVQYLLGLGLELMVKSAIVAWDAEADAKYLRNEVGHDLIAALDQAKARGFTS
                     SAPNLRALLDVLREPYKQHWLRYERPDEFELPSSFDEIVPILEALYSEVRTKLDAD"
     gene            4060..7170
                     /locus_tag="VN034_07020"
     CDS             4060..7170
                     /locus_tag="VN034_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60387.1"
                     /translation="MSEGPLGALGGLADKLASPLLSAVTSELARRGAGDIAVSSPATI
                     DQDFDEAIAMLMGEADTLPKQVVIWVKSRISEPPAQFHDPEAKDWLRRDDVRALLKRA
                     TLDLITIGTIDNHIEEAKELYATSSGEAGWYGEPLFIYAVSFIGLSLKAKASDDARLL
                     LVAGNHRAERTAEKIAEIHELVRAGNAEKDFTIAAPSGWSEVEFVSDRGLSAALTGQM
                     LGPADVSACPRLIEANTLLHQLEQANVARLAGAPGAGKSICMLQTARSLADKGWRVLR
                     LDDPAAKLPAIEESDQPTLYLIDNAHLISASQINRLEEGCGPDRRLLSVHTTGEVKTT
                     APGTIHLDAKRAVRTIAAGFQADFARTFEAVRQLDDRVGNRMFDEPLEHRLELAARAQ
                     LPWQFCFILSGGWRRAASEAASARAVGADLILAALAARQIAARDASCSMDEAMALLGP
                     LGSADEIKAAIAWLLERRLLLEPTDLRCPHQRFGSVLIREVLNGQDKAGRVAIAGMLR
                     DILRNPAFPLSGLATLLSEISSGAQYRGWARIIEPEWLDTAIEQLWAARTPGEIHEAA
                     WFALQLRSYRTDEMDIFRANADIIGAWIAQAPPQACYAVGDLVNHIFNTDEPFGAEIR
                     DGIEPDAIAQHIFPGDHRRAHEILSMTKSIRGTWDAGWSARFMAGLDRDAVRSLIQTW
                     PHDLGLSYAQSICEALTYMEEDFGLDLIEELAPAVAPIIERDPLESFHEIHDLFSFTL
                     RVLDILRLFVGKNGPDARRKALARKYAKLFARAPLAAKISACERRDFQNCAMLLSFLQ
                     RANPAAYHDVIRQLDWEKIDATIGEDWRVGSGDGEVFLGMAFGDKVARPAIKAVIERN
                     LDKIDRMPSRLALMFPDLAIKHLDAGRRIGFDSRWPIVAVLIVHFGAVRPDLLDQFIS
                     PEIEQLAADLSQPSPSFMNERLFFYRVLAAAAPDRLSDVLAQIDVEKARIGWTNALTG
                     KQNTKERGKKREARQVAAFIIEQSIGRDDACGELARVLRKRYPAASIPNAKTIEPLPP
                     FVPMKH"
     gene            8412..10106
                     /locus_tag="VN034_07025"
     CDS             8412..10106
                     /locus_tag="VN034_07025"
                     /inference="COORDINATES: protein motif:HMM:NF040700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPA1262 family N-terminal domain-containing
                     protein"
                     /protein_id="HWV60388.1"
                     /translation="MSHLLFASVELLPTEMQAPPNPAGGGWWGNFGDDRLSVSRTALS
                     LPDALDWYEKLKQGRATVPDKAFAIAASELGPEPDYNGFTILQEPPPFSPSWHGRPRL
                     HRLVPMGALAEPVQALRDSMTNVGAEGCARQWLRDHIHFDLLAYDDWLGSGVLVAPNP
                     LLRGFGARIVNRSVIPETLELGGTPRRGANVTSLQMVVEEVRAGAPAWRAGGSPNALG
                     RFRASAPAQVAMVREELFCPKRGVLDRVPPAFFFRSVSVSSSVVAEAKARHVDPPTRA
                     PDAGARIVYVRPAPSRQSEPSMTPLRALEFLQDARAERQGALRPAEAPQILPGVRLFE
                     HNREETVDWMCGLIARARSHVLFVDPYLDADDLQQFATATQYQGVAIRGLINPRSRRH
                     KRVDPNGDSFGDLMLKKIAAFRDPAQEFGEIDIRVSRGRRLHDRFLQIDDVIWHAGHS
                     FNKVGDGEISLMTLVAQPTELAQALAETFAEAEPFETWWANRPPTIWSLRREAGYQLR
                     RLAKWIDASGNSTITFGFGPRLFFGWPVRSALSAMGASNGRSAEMVGTFNKLLHWV"
     gene            10272..10347
                     /locus_tag="VN034_07030"
     tRNA            10272..10347
                     /locus_tag="VN034_07030"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10305..10307,aa:Thr,seq:cgt)
     gene            complement(10434..10790)
                     /locus_tag="VN034_07035"
     CDS             complement(10434..10790)
                     /locus_tag="VN034_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HWV60389.1"
                     /translation="METSSEAQGRVLSEAVARVAALWSIGDELLGEILGIAGNRATDL
                     RNGTFRLARPEPAFRAGQYLVRLYEELGALIGDDPAVIGWLQAENVDLGGRPIDLIRT
                     ERGLHELLTYLETRRA"
     gene            complement(10814..12031)
                     /locus_tag="VN034_07040"
     CDS             complement(10814..12031)
                     /locus_tag="VN034_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HWV60390.1"
                     /translation="MPLSDVQIRSLKPRDRVYKVSDAHSLYIEVHPTGARLWRFKYTM
                     HGKQKRIALGAYPNVGLAEARRKRDEAKLKIHEGTDPLAERKRHKLVGKFKASNCFGD
                     LAEEYIEMVTKEGRSEATIEKTKWLLEKLRPIAGTPIADLQAVDLFAVLKKIEAQGHF
                     ETARRCRSFASRVFRYAAASGRADSDPTTLLRGALIAPRPKHHAALVEPEEVGGFLRA
                     LDGYSGYPITLLACKMLPHVMARPGELRLARWAEFDFEKAVWTVPPERMKMRRPHYIP
                     LSRQVIALLEELRPYVGGEDYVFPAFHTWKRPLSENTMNAAYRRMGYGPRDLTSHGFR
                     ATASSLLNQCGKWNPDAIERSLAHADGNQIRRIYNRTPYWEERVRMHQWWSDYLDALR
                     SDDEAAITALLED"
     gene            complement(12254..12340)
                     /locus_tag="VN034_07045"
     tRNA            complement(12254..12340)
                     /locus_tag="VN034_07045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12304..12306),aa:Leu,seq:cag)
     gene            12542..>12708
                     /locus_tag="VN034_07050"
     CDS             12542..>12708
                     /locus_tag="VN034_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWV60391.1"
                     /translation="MQKFDGQLITVLGGGGFLGRYVVQRLLSRGARIRVAQREPRAAT
                     FLKPLGGLGQT"
BASE COUNT         2546 a         3806 c         3816 g         2540 t
ORIGIN      
        1 gatcccaccg atatcggcgc cgacgagatc aacgcacgca tggcgaagct cgccaagcga
       61 cgagcacagg gaagagccgc ctacgccgac gctaaatccc gcgacagcta atcgatggcg
      121 gcttcctgac cacgcttacg gggagatggg ctttgtcggt cacgctcgac ggtattttgc
      181 gccggcatag ccgggatatc gccttggttc tgggcaacgg catgaatcgg catgccaacg
      241 ctgcaacgct aaatagctgg gacggtcttt tgatgtccat tgcccgggat tgcatccccg
      301 gcgtcaattc cgtgcctccg ggcactgcgc tgaccgagtt ctacgatgtg gtcgagttaa
      361 agtcaccgat gggtgtgggc gagcttcaaa gccagttttg cgtgtcgatg aaagattggc
      421 gtcctgtcgc gcatcacatg cagatcgcag gctgggcgct gcgccatgat gtccccattc
      481 tcacgacaaa cttcgacgaa gtgatcagcc aagcggtagg gggtgatttt cagttcccga
      541 aggatccagc tttcaccgcc ttttatccgt ggggctgtca tttcggtccc gagttggttg
      601 acgatccgtg cgcaggcttc gccatctggc acatcaacgg aatggcgcgg tacaaaacca
      661 gcattaggct gggcctgacc cattatatgg ggtcggttca acgcgcgaga ggctggctcc
      721 acgggagcgg cgaagacaat ctgtttcgag caaaggatcg acgcgactgg aggggagccc
      781 ggacatggat gcacgtggtc ttcaacaagc ccctgctctt catcggcttg ggcctgactg
      841 agaatgaggt gtttctgcgg tggctactaa tcgagcgcgc caagtatttt cggcagttcg
      901 acgggaggaa gcatccggct tggtacgtct atgtcgacga tcccaaggat gaacggcagg
      961 ccggaaagct cttcttcctg gagtccgtcg gcgtccgatg ccttcaagca ccgagttacc
     1021 agacgatcta cgaagccgac ggatggggtg ggtagccaac gccatgaccg atgatgcgga
     1081 gaaagctgag ctggaccgcg accctgccat ttggcgagag ggcctcgata tacagcttgg
     1141 gctcgccgat gacgaagcct ttctgcggat ggtttgggga cttaatgcgc tgcagaccgg
     1201 tcgagccgac gttggccgcc cctatttccg caacgcccct cccgaggccg caaaagatgg
     1261 gattggcggc aagcattcgg tctacccctg ggagcttgag acactcgtca acgagcttct
     1321 cgtcacgccc aaacacgccc acggcccact gttcgactgc cgcgactgga gcgccatcgc
     1381 aacgctcgtc aacgagctcc gtggtcttga gaatgcggaa tatggtcagc gccgtgccga
     1441 acgctccatc ctccgtgaac tcgggcgtat cggggcgcgt caattcgaat ggcagcgcgg
     1501 atttttctcg atccagcaac tgtatcgcag cgcctatatt tacggccaga aggcctgcaa
     1561 cgaccatctg gtcgcgacga ccgggctgac aatgcctgtc ctcacgttgg tcggctttgc
     1621 ccttatgtcc tcctacctcg ggtccagtgc gatccagccg ggcagcgacc tcgcgttact
     1681 cgagagtctg ggagcctcac gtgacgaact tgaaaaagcg atcgcgctca tttctcggcc
     1741 acttcccgac ctacgaacag aggcgcggca gatacgttca gacggtgagc tgacggccta
     1801 taaagcgagc gtcttgcgca gatttccatg tgtcttggtg ggccggggca acagccaaat
     1861 gcttgcaccg ctgccggagc tcgtctcgaa ccggctgacc tcgggcctgt tctatgatgt
     1921 catcggaggc ggcggaccgg tgcgcgacga gaccgggcgg cagtttgaga gctatagcta
     1981 cgcgctcctc aatgcgatgc tgccggagtt ccagttcgag cgcgaatggg cctatcagac
     2041 ccgcctcggc caaatgaagt cgcccgacat catgttggca cttcccgatc agaccatcgg
     2101 cctcgcgatc gaatgcaagg cgctgcggat gacggtggca gcccgatttg gcgatgagcc
     2161 gcaggacgaa cgcggatatg aagaaatcgt ccggggcata ttccagctat ggcgcttctt
     2221 tgcgcattgc cgattggggc tggcgggacg ggatgtcacc gacgaggcgc ggggcgtcat
     2281 tttgacgctc gatgactggt tcgccgcacg ccactcgatc ctagaacaat tgctgaaacg
     2341 cgcacatgcc cgcgccgacg aagagaaggt ccctattctc ccaatcgatc gtcggccgat
     2401 cgccttctgc gcgatatcgg aaatcgagca gacactccgg actgcaacgg gcgcatctct
     2461 cctagccacg attgaactag cagctggcga gaagctcgga tggatctttc ccatggtcca
     2521 tcaagacttg aaagcgccaa aaacagagcc aaaagacttt ccgtttgaga acgaggtcgg
     2581 cgatcttttg ccatggtatc gccgcatagg cgaaatggcc gacgatggga cattcgccga
     2641 ttgagatgcg cgcatttaga tggcggcggg gcgaaccgcc gacacgacat aatccatatc
     2701 ctgaagctcg ccggcttcga gctgcgcgcg aagattcctc gcagcatcgc tgttgccgca
     2761 gaaagcgtca gcgtcgaaag gatatctcag cagcaacggc cccaaggtgc gatcggattg
     2821 cagcgcctcc gaaagcagcc tctgaacgct atcgaagatc gcgtcctgcc gctcggcgat
     2881 ctgagcgagg cggcgttcga gaagcagggc gaggcgctgg gcaagtataa ccttatgctg
     2941 tgcgcggacc gtgtagactc caatttgacg ctgagaccgc gtgaagctga caatgaccgc
     3001 ctcgcgaacg accggcccac cctcaagatc gtcgcctcga ccgaaatgtc cgatcacact
     3061 gttacgcata agccggactt cattgtgggt cgcgagctct tcgtccgaca gcccacgctc
     3121 gtttagaagc gacggccgct tgccagacga gattgtcgcg cgcgcgtaaa gaatgatggc
     3181 ctgcgtaaag agcgcgccgg tgatcgttgt ctgatcattg agctgcttca cttcgccccg
     3241 ctcttgttcg gcttgcatgt tctcgatgag gcggagtgcc gcgagggccg tgcggaggtc
     3301 ctccctgatc gatcccacgg ccgcggcgcg ctcaatcgcg cgcaccaggg gcacgtgccg
     3361 acccgctgcc gcgagcgctg cggcgccatg aacgaggtta aggcgtatcg catctttcat
     3421 tggacagccc tgcccttttg ctcggtcttc cgaacgaatg agcgtcgtca atgcgcggcg
     3481 acacgcacga ggtcaattct cgccttgccc atttcccgaa cgacaattca gtggcaatac
     3541 caagccatgg caactgagct agcagcgctt cttcttcgta acggcaaaga gcatgcggag
     3601 tccgcctgcc ttctttaccg ggctgcacac gacatcgcgc atgaagagaa tattgaagat
     3661 gaagaggctt ttgcgttcta cggaccctat tccttgtccg ttcaatattt gcttgggctt
     3721 ggcctcgaat tgatggtgaa gtctgccatc gttgcatggg atgccgaggc cgacgcaaaa
     3781 tatctaagaa atgaggtagg tcacgacctg atcgctgcgc tcgatcaggc caaagcgcgt
     3841 gggttcacat ccagcgcccc caatctgcgc gccctcctgg acgttctgcg cgaaccctat
     3901 aagcagcatt ggcttcgata tgagcggccg gacgaatttg agttgccgag cagctttgat
     3961 gaaatcgttc cgatcctcga ggccttatat agtgaggtgc gaacgaagct cgatgcggac
     4021 tgacgctcct cgctttacaa aaatggatgc ccggacgcga tgagcgaagg accattgggc
     4081 gcgctcggcg gattggccga caaactggcc tctccgttat tgagcgccgt caccagcgag
     4141 cttgcccgcc gcggcgcagg cgatatcgcg gtcagttctc cggcaacgat cgatcaggat
     4201 ttcgacgagg ccatcgccat gctcatgggc gaggccgaca ccttaccgaa gcaggtggtg
     4261 atctgggtca aatctcgaat ttctgaaccg cccgcgcaat ttcacgatcc cgaggcaaag
     4321 gactggctgc gccgcgacga tgtccgagcg ctcctgaagc gggcgaccct tgatctcatc
     4381 acgatcggaa caatcgacaa tcatatcgag gaagccaaag agctttacgc gacctcatcg
     4441 ggggaggcgg gctggtacgg cgagccactt ttcatctacg ctgtctcatt cataggcctg
     4501 agtctgaagg cgaaagcgag cgacgatgcg cgcctgcttc tcgtagcagg caatcaccgt
     4561 gccgagcgca ctgccgaaaa gattgccgaa attcatgagc tcgtccgggc cggaaacgcc
     4621 gagaaggact tcacgatcgc ggcaccgtca ggctggtcgg aagtagaatt tgtatcggat
     4681 cgcgggcttt cagcggcctt gaccggccaa atgctggggc cagcagatgt gtcggcctgt
     4741 ccgcgtctga ttgaagcgaa tacacttctg caccagctcg aacaggccaa tgttgcacgt
     4801 ctggcgggcg ctccgggcgc gggaaagtcg atctgcatgc tgcagaccgc gcgatcactc
     4861 gcggacaagg gctggcgcgt gctcaggctt gatgatccag ctgcgaaact cccggcgatc
     4921 gaggagagcg accagccgac attgtatcta atcgataatg cgcacctaat cagcgcctcg
     4981 cagatcaacc gcttggaaga ggggtgcggg ccggaccggc gactactctc ggttcatacg
     5041 accggcgagg tgaagacaac cgcaccgggc accattcacc tcgacgccaa gcgggccgtc
     5101 cggacgatcg ctgccggatt tcaggcggac tttgctcgga cgttcgaagc ggttcgccaa
     5161 ctggatgacc gggtcggaaa ccggatgttc gatgaaccgc tcgaacatcg tctggaacta
     5221 gccgcgcgtg cacagctgcc atggcaattc tgtttcatcc tgagcggggg ttggcgccgt
     5281 gcggcaagtg aggcggcgtc cgcgcgggcc gtcggagcag atttgatatt ggccgcgctg
     5341 gcagcgcgtc agattgcggc ccgcgatgcg agctgttcga tggacgaagc catggcgctt
     5401 ctgggtccgc tcggttccgc cgatgaaatc aaggctgcga ttgcatggtt gctggaaagg
     5461 cgtcttctgc tcgagcccac cgaccttcga tgcccgcatc aacgctttgg atcggtcctc
     5521 atccgagaag tgctgaacgg gcaggacaaa gccgggcgcg tagcgatagc gggcatgctt
     5581 cgcgatatcc ttcggaatcc ggccttcccg ctttccggcc ttgccacatt gctcagcgag
     5641 attagttcgg gggcgcaata tagaggttgg gcgcgcatca tcgagcccga atggctcgac
     5701 acggccattg aacaactctg ggccgcgaga acgccgggcg aaatccacga agcagcttgg
     5761 ttcgcgcttc aattgcgaag ctatcgcacc gacgaaatgg acatcttccg cgccaatgcc
     5821 gatatcatag gagcctggat cgcccaggcg ccaccgcaag cctgttacgc cgtcggcgat
     5881 ctggtcaacc atattttcaa taccgacgaa ccctttggtg ccgaaatacg tgacgggatc
     5941 gaaccggacg ccatcgcgca gcatattttc ccgggcgatc atcgccgggc tcatgagatt
     6001 ttgagcatga cgaagtcgat tcgcggcaca tgggatgctg gctggtcagc gcgcttcatg
     6061 gcgggactcg atcgcgatgc cgttcggtcg cttatccaga catggccgca cgatctcggc
     6121 ctctcctatg cgcaaagcat ctgcgaggcg cttacctaca tggaagagga ttttggcctc
     6181 gatctaattg aagagctggc accggcggtc gcaccgatca tcgagcgtga tcctcttgag
     6241 agctttcacg agatccacga tctattttcc ttcacgttgc gggtgctcga catcttgaga
     6301 ttgttcgtcg gaaaaaacgg ccctgacgcg cgccgaaagg cattggctcg gaaatatgcc
     6361 aagctgtttg cccgagcgcc gctcgcggcg aagatctctg cctgcgagcg gagagatttc
     6421 cagaattgtg caatgctgtt gtcatttctt cagcgtgcaa atcccgcagc ctatcacgac
     6481 gttatacggc agctcgattg ggagaaaatc gacgccacga tcggcgagga ttggcgggtt
     6541 ggctcgggcg atggagaggt tttcctcggg atggcctttg gcgacaaagt cgctcggccg
     6601 gcaatcaaag cggtgatcga acgcaatctg gacaagatcg atcggatgcc ttcgcgcctc
     6661 gcactcatgt tccctgactt ggcgatcaag cacctcgatg ccgggcgtag gatcggcttc
     6721 gactcccgct ggccaatcgt cgccgtgctc attgttcatt tcggggcagt caggccagac
     6781 ctcctagacc agtttatctc gccggagatt gagcagctcg ccgcagatct ctcgcagccc
     6841 agtccgagct tcatgaacga acggctcttt ttttacaggg tgttagccgc agccgcgcct
     6901 gaccggctgt ccgatgttct tgcccagatc gatgtcgaga aggcgaggat aggctggacg
     6961 aatgcattaa cgggcaaaca gaatacgaag gaaaggggca agaagcgcga agctcgccaa
     7021 gtcgcggcct tcatcatcga gcagtcgatc ggtcgcgacg atgcctgcgg cgaattggcg
     7081 cgcgtgctgc gaaagcgcta cccggccgcg tcaatcccga acgcgaagac gatcgagcca
     7141 ctcccgccct tcgttccgat gaagcattga ccacgtaggg atggcagcgt cgcagcgggc
     7201 gctaccggga ctccgatagt ggggtcaccc gatgcgacgc taccatttga agagacgctc
     7261 ggaattcaag tttgcggaaa tgcgcccgga gcccaagatt acgtggccat ttgacttatg
     7321 cgtctacaca catgagcaag ccttgctttg tctggggagc aaatgagtgg ccgccgacga
     7381 tcgcagaccg tgcccgcatc tcgatgagcg cgtctaagac cctctacctc gagtccgatc
     7441 gcatgggcta tccgatcggg ccgagcctat ttcttaacaa tcgcgaaaat gattctgctg
     7501 caacgcatcc ggcatcacaa gatcgtccgt atcgttacga tgaccaagat ggaagcgatg
     7561 acaatacccg atgatgcgcc agggcccgag gaacaggtga catcacgaat ggagcttgag
     7621 cgcgttcgaa agatcatcgc aagtctaaga aagcggcgac aaatgggttc acatcgtaat
     7681 tcggcctagt acgcgaactc tgatcggggc gaggcggaac ggttgatgtg cctgtcccat
     7741 accaagttcg gtacgctaac gacagcttgc gcgtttgaag cctatctggg accggcaata
     7801 ccggggcgcg gtgccgcaaa cgcctgtcct aaggtccgct ttcgggaaca ggcgcggact
     7861 atcaaaatgg cgtagtacga ggcgcgatgc ggccaagccg ggaatcacat gaatttcagg
     7921 cagtcgccac gcggttccgc cgaaaattcc gcgcgcacgg aagtgcgtgg tcttgggatc
     7981 cagtggagtg tcggaaaacc actgtcggat ttagtaaagt gtccgtcgac gctggcagcg
     8041 gggtttgctg gcgagggatg tcaggcatat tgtctttgta ggtcaccgtc tttcgctacg
     8101 acgcgctatg gtcgccggcc ccgatgtgat ccatggctta aggggtgttt cctgctccga
     8161 aaaggctaac agaagcagga gtcggtccga aagcacggcg cccaatttgc cgataagttg
     8221 ggataaggcg ctctattagt tcgcttcgaa agatatctga ctcgcgtcca gtgacagctt
     8281 caaccatatt ccatctcatg caatcgactg atgcccctag accggcgtcg ccgctggcgc
     8341 gatggcggcc tcttgttcct ggcgactaca acacgggcgt agtgcgccta gtcacgcttg
     8401 tgcgcgaccg ggtgagccat ctgctgttcg cttcggttga gctgttgccg accgaaatgc
     8461 aggctcctcc aaatccggct gggggcggct ggtggggcaa ctttggtgac gaccggctat
     8521 ccgtgagccg gaccgcgctg tcgttgccgg atgcgctcga ctggtacgag aagttgaaac
     8581 aggggcgcgc tactgttccg gacaaggcct tcgcgatcgc cgcctcggag ctaggtcctg
     8641 aacccgacta caacggcttt acgattctcc aggagccgcc gcccttttcg ccatcatggc
     8701 acggccggcc gaggctacat cgcttggtgc cgatgggggc gctggccgag cctgtgcaag
     8761 cgctgcgcga cagcatgacg aacgtcggcg ccgaaggctg cgcccggcaa tggcttcgtg
     8821 accacatcca ttttgattta ttagcttatg atgactggct gggttcgggc gttctggttg
     8881 caccaaatcc gttgctgcgc ggctttggcg cgcgcatcgt caatcggtcg gtgataccag
     8941 agactctaga gcttggcggc accccgcgac ggggtgccaa tgtcacgtcc ctgcaaatgg
     9001 tagtcgaaga ggttcgcgcg ggagcgccgg cgtggcgcgc agggggctca ccaaacgccc
     9061 tcggacgctt ccgggcttcg gcgcccgccc aagttgccat ggtgcgcgag gagttgtttt
     9121 gtccaaagcg tggcgtattg gaccgcgtgc cgccagcgtt cttcttccgc agcgtctctg
     9181 tctcctcctc tgtagtggct gaggccaagg cccgtcacgt cgatccaccg actcgcgccc
     9241 ctgatgcggg agcgcgcatc gtgtatgtcc gacccgcccc atcgcggcaa tctgagccct
     9301 ctatgacacc tctgcgggcg ctcgaattcc tccaagacgc gcgggccgag cggcagggcg
     9361 cgctacgacc tgcggaggcc ccgcaaatcc tgcccggagt tcgcctgttt gagcacaatc
     9421 gcgaggagac agtggattgg atgtgtgggc tgatcgcccg cgcacgctct cacgtcctat
     9481 tcgttgaccc ttacttggac gctgacgacc tgcagcaatt cgcgacggcc acccagtacc
     9541 aaggcgttgc gatccgcggt ctgatcaatc ctcgctcccg ccgtcacaag cgtgtcgacc
     9601 ccaacggcga tagcttcggg gatctcatgc tgaagaaaat tgcggccttc cgcgacccgg
     9661 cccaagagtt cggcgagatc gacatccgag tgtcccgggg acgccgcctg cacgaccgct
     9721 tccttcagat cgacgatgtc atctggcacg ccggccattc gttcaacaag gtgggggacg
     9781 gagagataag tctgatgacc ctcgtcgccc aacctactga attggcacag gccttagcgg
     9841 agactttcgc cgaagccgag ccattcgaga catggtgggc caatcgaccg ccgacaattt
     9901 ggtcgttaag gcgcgaggcg ggttatcaac tgcgccgcct ggcaaaatgg attgacgcct
     9961 cgggaaattc gacaatcacc ttcggtttcg ggccgcgcct ctttttcgga tggccagtga
    10021 ggtccgcgct ctcagctatg ggagcgagca acgggcgttc cgccgaaatg gtgggcactt
    10081 tcaataaact cctgcattgg gtgtagggcg gcgcccgatg gcagaagctt cctttcaaat
    10141 ggaaaacggc tggatcgctc tcgcaattgc tgcgaactac agcgcccgaa caccctctcg
    10201 cgctcgctgc cgggaaactg cgctacgacg cttgtcttcg cgccgacacg ctggtagagg
    10261 cgcccctcgt tgccgcttta gctcagttgg tagagcacat cattcgtaat gatggggtca
    10321 caggttcgag tcctgtaagc ggcaccatcg accccataaa tcaatgactt aggcccgatg
    10381 aagtttcttc gggagccgta atgcgcggtc aataggcgcc acctgaatca ggactatgcc
    10441 ctccttgtct ccaaataggt cagcagctcg tgcaagccgc gttcggtccg gataaggtcg
    10501 atcggccgac cgccaagatc cacattctcc gcttgaagcc agccaatgac ggccgggtca
    10561 tcgccaatca gcgctcccaa ctcctcatac agccgcacga gatattgacc ggcacggaag
    10621 gctggttcag gtcgagcaag tcggaatgtc ccgttacgca agtccgttgc gcgattgccc
    10681 gctatgccga gtatctctcc aagcaactcg tcaccgatgc tccagagcgc agcaacgcgc
    10741 gccacagcct cggacagcac gcggccctgc gcttccgacg atgtctccat catctcctcg
    10801 ttccgatcca cggtcagtcc tccagcaagg cagtgatcgc cgcttcatca tcgctgcgca
    10861 gcgcatccag atagtcgctc caccattggt gcatccgtac ccgctcctcc caatagggcg
    10921 tccggttgta gatgcgccgg atctgattgc cgtcggcatg cgcgagcgac cgctctatcg
    10981 catcggggtt ccacttgccg cactggttga gaagcgacga ggcggtcgcg cgaaatccat
    11041 gggacgtcaa atcccgcggc ccgtagccca tacggcgata ggccgcattc atcgtattct
    11101 cgctgagcgg tcgcttccac gtgtggaacg ccggaaagac ataatcctca ccgccgacat
    11161 agggccgaag ctcttccaac agggcgatca cctgccgcga caacgggata tagtgcggtc
    11221 ggcgcatctt catgcgctcc ggcgggacag tccagacggc cttctcgaaa tcgaactcgg
    11281 cccagcgcgc gagccgtagt tcaccgggcc tcgccatgac gtgcggcaac atcttgcagg
    11341 cgagcaaggt gatcggatag cccgagtatc cgtctagcgc gcgcagaaaa cctcccacct
    11401 cctccggctc gacgagagcg gcgtggtgct tcggcctcgg cgcgatcagc gcgccgcgca
    11461 gaagcgttgt cggatcggag tccgctcgcc ccgaggccgc agcatagcga aacacacggc
    11521 tcgcaaagga gcggcaccga cgcgccgtct cgaagtgccc ctgcgcctcg atcttcttga
    11581 gcaccgcgaa gagatcgacg gcctgcaggt ccgcgatggg cgtgccggca atcggccgaa
    11641 gcttttcgag aagccacttc gtcttctcga tcgtcgcttc gctccggcct tccttggtga
    11701 ccatctcgat atattcctcg gcgagatcgc cgaaacaatt cgaggccttg aacttgccga
    11761 cgagcttgtg acgcttccgc tccgcgagcg ggtcggtgcc ctcatggatt ttcagcttgg
    11821 cctcgtcgcg cttgcgccgg gcttcggcga ggccgacatt cggataggcg ccaagcgcaa
    11881 tgcgcttctg ctttccgtgc atggtgtatt tgaaccgcca caggcgcgca ccggtcgggt
    11941 gcacctcgat gtagaggctg tgggcatcgg agactttgta gacgcggtcc cgcggtttga
    12001 gggacctgat ttgaacatcg ctaagaggca cggctcaact cctttaaaag ggttaggacc
    12061 ggcaccaatc acagcgctcg aggcgaacgg gccatcgagc aaaactctct ccaaaaaaga
    12121 cgggccattt tggccttttt gatggcccgt ttcgggttga gattttgtga gacaccctcg
    12181 gacaattcgg aacaaaaccc acggaaaact aacatattcc ggggattttg tcgattgccc
    12241 tgagaagggt aaatggtgcc cagaagagga ctcgaacctc cacgaccttg cgatcgccag
    12301 cacctgaagc tggtgcgtct accaattccg ccatctgggc acggggtagg agcgggcgct
    12361 tagcggcgtg gccatcagct tgtcaaccgc ggcgcgggag tcgaggcaca ttttatcgca
    12421 aactcgtcgc tttcccgcgc cggacgcgcc ttcgcccctt gcccccttcc cggcttcgcg
    12481 gcatggggaa gccgtgcggc gcgccctgcg ccggtaaaag aatcaccgac aggcggacgc
    12541 aatgcagaaa ttcgacggac aattgatcac ggtattgggc ggcggcggct tcctcggccg
    12601 ctatgtcgtg cagcggctgc tttcgcgcgg cgcccgcatc cgcgtcgccc agcgcgaacc
    12661 gcgtgccgcg actttcctga agccgctcgg cggtctcggc cagacgca
//
LOCUS       DATMSU010000262         1757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_118528,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000262 DATMSU010000000
VERSION     DATMSU010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118528"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..612)
                     /gene="dcd"
                     /locus_tag="VN034_07055"
     CDS             complement(58..612)
                     /gene="dcd"
                     /locus_tag="VN034_07055"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWV60392.1"
                     /translation="MSILSDKWIREAARTQGMIEPFVEAQRRDGCISYGLSSYGYDAR
                     VAPEFKIFTNVDSAVVDPKDFASNSFVDRETDVCIIPPNSFALARTVEYFRIPRDILV
                     ICLGKSTYARCGIIVNVTPLEPGWEGHVTLEFSNTTPLPAKIYANEGACQFLFLKGNE
                     PCETSYADRAGKYMGQKGVTLPKL"
     gene            complement(652..1113)
                     /locus_tag="VN034_07060"
     CDS             complement(652..1113)
                     /locus_tag="VN034_07060"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWV60393.1"
                     /translation="MTEIHPDTRDALIDAARDAASRAYAPYSGFHVGAALLLRNGDVV
                     TGANVENASYGLTLCAETAAVAKIANEGWIGELAEVAIVGGRPDGDALLGGDPVHPCG
                     RCRQILNEAAERSKTDVLVHCASGDGTAVKSYRLSALLPAAFGPKDLGLID"
     gene            complement(1110..>1757)
                     /locus_tag="VN034_07065"
     CDS             complement(1110..>1757)
                     /locus_tag="VN034_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HWV60394.1"
                     /translation="LLAAGLALWWAARWTPDRAIYPTQGVTIDAGNGDVHWGSIKAAG
                     AEFAYIAATDGSGGIDAKFARNMAEAREAGVQAGAIHRYSLCQLATDQAANFIRHVPR
                     RADMLPAVVWLDYDDRCPDRPTRALLLSELATFLAQIEAHMGKQSLIAPGPAFESDYK
                     VTRGIARTTWLRRDFFEPDYGAHPWAMWQANDYVRLSGARGTVGWNVLRPQGEMR"
BASE COUNT          297 a          553 c          604 g          303 t
ORIGIN      
        1 cgggatgacg aaattggtga taagcccggt gctaagggaa atgtgaagga gcgccgctta
       61 aagcttgggc agcgtgacgc ccttctgccc catatatttg cccgcgcggt cggcgtagct
      121 cgtctcgcac ggctcgttgc ccttgaggaa gaggaactgg cacgcgccct cattggcgta
      181 gatcttggcg ggcagggggg tggtgttcga aaattcgagc gtcacatggc cctcccagcc
      241 gggttcgagc ggggtgacgt tgacgatgat gccgcagcgc gcataggtcg atttgccgag
      301 gcagatcacg agtatgtcgc gcggaatgcg gaaatattcg accgtgcgcg cgagcgcgaa
      361 gctgttcggc gggatgatgc acacgtcggt ttcgcggtcg acgaagctgt tcgacgcgaa
      421 atccttggga tcgaccaccg cgctgtcgac attggtgaaa atcttgaact cgggcgcgac
      481 gcgcgcgtca tagccgtagg aggagaggcc gtagctgatg cagccgtcgc ggcgctgcgc
      541 ctccacgaac ggctcgatca tgccctgcgt ccgggcggct tcgcgaatcc atttgtcgga
      601 aagaatgctc atgcggctgt cttgtcggtc ctgcggttat tgcgcaagcg gtcagtcgat
      661 gagccccaaa tccttcggcc caaaggcggc aggaagcagt gcggagagcc tgtagctctt
      721 caccgccgtc ccgtcgcccg aggcgcaatg gacgaggaca tcggtcttcg accgttcggc
      781 agcttcgttg agaatctgcc ggcagcgccc gcacggatgg accgggtcac cgccgagcaa
      841 ggcgtcgccg tcgggacggc cgccgacgat cgcgacctcg gcgagttcgc cgatccagcc
      901 ttcgttggcg atctttgcga cagccgcggt ttcggcgcag agggtgaggc cgtagctggc
      961 gttctcgaca ttcgcgccgg tcacgacatc gccattcctg agcagcagcg ccgcaccgac
     1021 atggaaaccc gaataagggg cgtaggcgcg gctcgcggcg tcgcgcgcgg catcgatcag
     1081 cgcgtcgcga gtgtcggggt ggatctcggt catcgcatct ctccttgcgg gcgcagcacg
     1141 ttccagccga cggtgcccct ggcgcccgac aggcggacat agtcgttcgc ctgccacatc
     1201 gcccagggat gcgcgccata atcgggttcg aagaaatcgc ggcgcagcca cgtcgtgcgc
     1261 gcgatccccc gcgtcacctt ataatcgctc tcgaacgccg ggccgggggc gatcaggctc
     1321 tgcttgccca tatgcgcctc gatctgcgcc agaaaagtcg cgagttcgga cagcaggagc
     1381 gcgcgcgtcg gccggtcggg gcagcgatcg tcataatcga gccagacgac ggcgggcagc
     1441 atatcggcgc ggcgcggcac atggcggatg aaattggcgg cctgatcggt cgcgagctgg
     1501 cagaggctgt agcgatggat cgcgcccgcc tgcacgcccg cctcgcgcgc ttcggccatg
     1561 ttgcgggcga atttcgcgtc gatcccgccc gacccgtcgg tcgccgcgat ataggcgaac
     1621 tcggcgcccg cggccttgat cgagccccaa tggacgtcgc cgttgccggc gtcgatcgtc
     1681 acgccctgcg tcggatagat ggcgcgatcg ggcgtccagc gcgccgccca ccacagcgcg
     1741 aggcccgcgg caaggag
//
LOCUS       DATMSU010000263         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_52077, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000263 DATMSU010000000
VERSION     DATMSU010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52077"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VN034_07070"
     CDS             complement(<1..629)
                     /locus_tag="VN034_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWV60395.1"
                     /translation="MILALTLAVVLGTMAGALLSARRQERARTLTEWAVGGRRFGVLV
                     FWFLNAGEIYTTFAVLGISGFAWAHGAPAYLAFTSVSLSAAIGYWLMPCIWNAGRRYG
                     LVTQADFFAAHYRATWLGVVVGIAGIAALIIYVQIQIVALSLIVRLTLGPEVSTAAAA
                     TVAAAIMLAFVYFAGLRSAAFAAGVKDVLMLLIVVALCATVASKVGAAS"
     gene            complement(626..901)
                     /locus_tag="VN034_07075"
     CDS             complement(626..901)
                     /locus_tag="VN034_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HWV60396.1"
                     /translation="MPEETELLSATSPVSRRHRWLLLIPFLWQVGAVPLVNDVAWRPF
                     SLPFPMVWQMAGILVTSAIIAFIFAIDRRHPEFGDDDDASGQAGANP"
     gene            complement(916..2727)
                     /locus_tag="VN034_07080"
     CDS             complement(916..2727)
                     /locus_tag="VN034_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWV60397.1"
                     /translation="MQHIRPFQLARRAMIGMMAASALSPLHGFAKTGSSAEALRALLE
                     RSEAADAALDPLRAARNGGGTGGPLFVDPLSDGYGATLRANKTRDLAELRRIDRATLS
                     PVDRIAYDVFDYKTRQMLALFDGGLFAVQRKAPLNPSFGLHVEFPDFLSGGAAPFATA
                     RDYDDALARIDGFVDYMYSTVARLKEGRAEGYLQPKIIVTNILAQVDAMLALPVEKSP
                     FWSGIDRMPDAFTPAERTRITAGFRAAINEKVYPAYRAWGTYLRDTYLADAPDAPGLW
                     AMKDGAALYDWNLARHTTTTRSADDIHTLGLSEVARIRGEMEKIRTEVGFAGDLKAFF
                     EHVRTDPRYYYKTPAELLARFEAIEAKIWPAIPRLFHDRPKAPFEVRPLPALGDQRGT
                     GYYRPGPPDGVSPGILFFNMSMLNTRPIPTLETLTLHEGIPGHHFQLTLAIENSELPP
                     LLRHGQATAYTEGWGLYAESLGPELGMFTDPLQMFGHLDMEMLRAVRLVVDTGIHAKR
                     WGRQQAIDYMLDNTSMAPRDVVVEIDRYIAYPGQACAYKIGELKFRQLREEASKALGA
                     AFDIRDYHHQVLNTGALPMEVLETKIRAWIASGGGRA"
BASE COUNT          566 a          953 c         1028 g          527 t
ORIGIN      
        1 atcgacgccg cgccgacctt cgacgcgacg gtcgcgcaga gcgcgacgac gatcagcagc
       61 atcagcacgt ccttcacccc cgcggcgaag gcggccgagc gcagcccggc gaaatagacg
      121 aaggcgagca tgatggccgc ggcgaccgtc gccgcggccg ccgtcgaaac ttcggggccc
      181 agcgtcagcc gcacgatcag gctgagcgcg acgatctgga tctgcacata gatgatcagc
      241 gccgcgatcc ccgcgatacc gaccacgacg ccgagccatg tcgcgcgata gtgcgcggcg
      301 aagaaatcgg cctgggtaac cagcccgtat cggcgcccgg cattccagat gcacggcatc
      361 agccaatagc cgatcgccgc cgagagtgag accgacgtga aggcaagata ggcgggcgcg
      421 ccatgtgccc atgcgaaccc cgaaatgccg agcaccgcga aggtcgtgta aatctcgccg
      481 gcattgagga accagaagac gagcacgccg aagcggcggc caccgaccgc ccattcggtg
      541 agcgtgcgcg cgcgctcctg tcgccgcgcc gacaggagcg ctccggccat cgtgccaagc
      601 acgaccgcga gggtgagggc taggatcaag ggtttgctcc cgcctgtccg gaggcgtcgt
      661 catcgtctcc gaattcagga tgccggcggt cgatcgcgaa gatgaaggcg atgatcgccg
      721 aggtgacgag gattccggcc atctgccaaa ccatcgggaa aggaagcgag aagggacgcc
      781 aggccacatc gttgaccagc ggaaccgcgc cgacctgcca cagaaagggg atgagcagca
      841 gccatcggtg gcggcgtgaa acgggggatg ttgcggaaag caattcggtt tcctcgggca
      901 tcggacgcgg catcctcaag cccgcccgcc gcccgatgcg atccacgcgc gtatcttggt
      961 ctcgagcacc tccatcggca gcgcgccggt gttcagcacc tgatggtgat agtcgcgaat
     1021 atcgaacgcg gcgccgagcg ccttgctcgc ctcctcgcgc agctggcgaa atttgagctc
     1081 gccgatcttg taggcgcatg cctgccccgg ataggcgata tagcggtcga tctcgaccac
     1141 cacatcgcgc ggcgccatcg acgtgttgtc cagcatatag tcgatcgcct gctggcgtcc
     1201 ccagcgtttc gcgtggatgc cggtatcgac gacaagccgc accgcacgca gcatttccat
     1261 gtcgagatgg ccgaacatct gcagcgggtc ggtgaacatg ccgagttcgg ggccgagcga
     1321 ttcggcgtag agaccccagc cttcggtgta ggccgtggcc tgcccgtgcc ggagcagcgg
     1381 cggcaactcg gaattttcga tcgcgagcgt cagctggaaa tgatgccccg gaatcccctc
     1441 gtgcagcgtc agcgtctcga gcgtcgggat cggccgcgtg ttcagcatcg acatgttgaa
     1501 aaagaggatg cccggcgaca cgccgtcggg cggtccgggg cggtaatatc cggtgccgcg
     1561 ctgatcgccg agcgcgggca gcgggcggac ttcgaacggc gctttgggac gatcatggaa
     1621 cagccgcgga atcgccggcc agatcttcgc ctcgatcgcc tcgaaccgcg cgagcaattc
     1681 ggcgggagtc ttgtaataat atcgggggtc ggtgcggaca tgttcgaaaa acgccttgag
     1741 gtcgcctgcg aagcccactt cggtacggat cttttccatc tcgccgcgga tacgcgcgac
     1801 ttcggacagg ccgagcgtgt ggatgtcgtc ggccgagcgt gtcgtcgtcg tgtgccgcgc
     1861 gagattccag tcgtagagcg cggcgccgtc cttcatcgcc cacagacccg gcgcgtcggg
     1921 cgcgtctgcc agataggtat cgcgcagata ggtgccccag gcccggtagg cggggtagac
     1981 cttctcgttg atcgcggcgc ggaaccccgc cgttatccgg gtgcgttcgg cgggagtgaa
     2041 cgcatccggc atccggtcga tgcccgacca gaaggggctt ttttcgacgg gcagcgcgag
     2101 catcgcgtcg acctgcgcga ggatattggt gacgatgatc ttcggttgca gatagccttc
     2161 ggcgcggcct tccttcaatc gggcgacggt gctgtacata tagtccacga aaccatcgat
     2221 gcgtgcgagg gcgtcgtcat agtcgcgggc ggtcgcgaac ggcgcggcgc cgcccgaaag
     2281 aaagtcggga aactcaacgt gcaggccgaa ggaggggttg agcggcgcct tgcgctgcac
     2341 agcgaacagt ccgccgtcga acagcgcaag catctggcgc gtcttgtagt cgaatacgtc
     2401 ataggcgatg cgatcaaccg gcgacagcgt cgcacgatcg atccggcgga gctcggcaag
     2461 atcgcgcgtc ttgttggcgc gcagggtcgc gccatagccg tcgctcagcg ggtcgacgaa
     2521 aagagggcct cccgttccac cgccgttgcg tgccgcgcgc aacggatcga gggcggcatc
     2581 ggcagcctcg ctgcgttcga gcagtgcgcg cagcgcctcc gcgcttgaac ccgtcttcgc
     2641 gaagccatgc agcgggctca gcgcgctggc cgccatcatc ccgatcatcg cgcggcgcgc
     2701 gagctggaat ggccgtatat gctgcatgat cgagtccccg ccttgttttc gagttgttgc
     2761 atgatcgtgt cgcgacgcgg caagaatgca accggcggcg tgacagagct cgatcggcat
     2821 ggctggcggt gaagcagcga aaagcagcga ttttccgcgt ttatcgttgt cgtttctgaa
     2881 agcttgttta agttacgttg cgtttactgc aacgaacttg cgccttcttg cgcattgttt
     2941 tcttgagcca ttgccgcagg tttggctgca gatactaggg aagggcgatt gatgaaacga
     3001 ctgcatgccg ggccggcgct tgccgcattt ctgggactat ttgctgcatc ggcggcggct
     3061 cagccggccg aagc
//
LOCUS       DATMSU010000264         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_118536,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000264 DATMSU010000000
VERSION     DATMSU010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118536"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VN034_07085"
     CDS             complement(<1..240)
                     /locus_tag="VN034_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HWV60398.1"
                     /translation="MALGPRLDLRQSQSLVMTPQLQQAIKLLALSNLELEAYLAEALE
                     GNPLLDTASADSDGSAGDPEFTPGGDAPTTEAASLD"
     gene            complement(256..1071)
                     /gene="lptB"
                     /locus_tag="VN034_07090"
     CDS             complement(256..1071)
                     /gene="lptB"
                     /locus_tag="VN034_07090"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWV60399.1"
                     /translation="MTDDESTVGTLAEGDHGVTEHRAHVREDAAAGNGLAVISIAKSY
                     DKRVVLSDVSLSVGKGEVVGLLGPNGAGKTTCFYSIMGLVKPDAGRIMLDGADITALP
                     MYRRSILGLGYLPQETSIFRGMTVAQNIEAVLELAEPDKIARERRLEELLDEFGLTRL
                     RDAAAMALSGGERRRAEIARALAANPSIILLDEPFAGIDPLSISDIRDLVADLKTRGI
                     GVLITDHNVRETLDLVDRACIIYDGKVLLAGSPAELVADPEVRRLYLGEGFSL"
     gene            complement(1299..2681)
                     /locus_tag="VN034_07095"
     CDS             complement(1299..2681)
                     /locus_tag="VN034_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWV60400.1"
                     /translation="MTHNFHPTMLREYDIRGVVGDTLSDKDAYAIGRSFATLIRRAGG
                     KRVAVGYDGRLSSPMLADALIAGINAAGIDALNVGLGPTPMLYYAASTEDVDGGIQIT
                     GSHNPPYYNGFKFVMQGRPFYGADILGIGKMAAAGDWDSGEGTSQSIDIVDAYVDRLV
                     EGFAGGAFRIGWDAGNGAAGTVIEKLTARLPGEHHLLFTDIDGHFPNHHPDPTEEKNL
                     ADLRKLVAEKSLDFGVAFDGDGDRIGAIDGEGRVIWGDQLLQIYAAAVLQDMPGATVI
                     ADVKASQALFDRVAELGGKPLMWKTGHSLIKAKMKETGSPLAGEMSGHIFFADRYYGY
                     DDAPYAAVRLIEAATKLGKSVTQLRSEMPAMVNTPEMRFQVDESRKFAIVDEVLDRLA
                     ETGAKVDRTDGARVLTDDGWWLLRASNTQDVLVARAEAKDEAALARLLAAIDEQLALS
                     GIVRGESVAH"
     gene            complement(2711..3520)
                     /locus_tag="VN034_07100"
     CDS             complement(2711..3520)
                     /locus_tag="VN034_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HWV60401.1"
                     /translation="MTTPAPHRIIFANEKGGTGKSTCAVHFAVALASQGWRVAGIDLD
                     PRQRTFHRYLENRENTAKRREIALPTPRFEVFTGSTIEELDEQVAALSEDVDYLIADT
                     PGRDDVFARHLATSADTLVTPINDSFIDFDLIGQVDPETFQVTRPSFYSELIWDTRKA
                     RAKSDGRTIDWIILRNRLQHVDAHNMRRVGEALTQLSRRVGFRVIPGLGERVIYRELF
                     PAGLTLLDKAHLGGMGIGHVVARQELREAMGGLNLPARERFHPDGDLFAAA"
     gene            3607..>4271
                     /gene="panC"
                     /locus_tag="VN034_07105"
     CDS             3607..>4271
                     /gene="panC"
                     /locus_tag="VN034_07105"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWV60402.1"
                     /translation="MQIIRDIAMLHRAVTALKQGGKSVALVPTMGSLHDGHLSLVRMG
                     RRVADHAVVSIFVNPTQFGPNEDFDAYPRDEARDAAMLVDEGASLLWAPDVATMYPGG
                     HSTHIEVAELGADYCGAARPGHFDGVATVVAKLFNQVRPDVAIFGEKDWQQLAIIRRM
                     ARDLDFGIDILGAPIARDSDGLAMSSRNAYLSDAQRAAAPAFPAALNAAAKVIAGGAD
                     VAEA"
BASE COUNT          703 a         1401 c         1398 g          769 t
ORIGIN      
        1 gtcgagcgac gcggcttcgg tcgtcggtgc gtcgccgccg ggcgtgaatt cgggatcgcc
       61 cgcgctgccg tcgctgtcgg ccgaggcggt gtcgagcagc ggattgccct cgagcgcctc
      121 ggccagatag gcttcgagtt cgaggttcga cagcgccagc agcttgatcg cctgctgcag
      181 ctgcggcgtc atcaccagcg actgcgactg gcggagatcg aggcgcggac ccaacgccat
      241 cagcgtgtca tcgcattaca aggagaaacc ctcgccgaga tagaggcggc gcacctcggg
      301 atcggcgacg agttcggccg gggagccggc aagcagcacc ttgccgtcat agatgatgca
      361 cgcgcggtcg acgaggtcga gcgtctcgcg cacattatgg tcggtgatga ggacgccgat
      421 gccgcgcgtc ttcaaatcgg cgacgaggtc gcgaatgtcg ctgatcgaca gcgggtcgat
      481 gcccgcgaag ggttcgtcga gcaggatgat cgacggattg gcggccagcg cgcgcgcgat
      541 ttcggcgcgg cggcgttcgc cgcccgacag cgccatagcg gcggcgtcgc gcagccgcgt
      601 gaggccgaat tcgtcgagca gttcctcgag ccgccgttcg cgcgcgatct tgtcgggctc
      661 ggcgagttcg agcacggcct cgatattctg cgcaaccgtc atgccgcgaa agatcgaggt
      721 ttcctgcggc agataaccga ggccgaggat cgagcggcga tacatcggca gcgcggtgat
      781 gtcggcgccg tcgagcatga tgcgcccggc gtcgggcttc accagcccca tgatcgaata
      841 gaaacaggtc gtcttgcccg cgccgttcgg tcccaaaagg ccgacgacct cgcccttgcc
      901 gaccgacagc gacacgtccg acagcacgac gcgcttgtca tagcttttcg cgatcgagat
      961 gaccgcgaga ccattgcccg ccgccgcatc ctcgcgaaca tgcgcgcgat gttcggtgac
     1021 gccatgatcg ccctcggcca gcgtgccaac agtggattcg tcgtcggtca taggtcggtt
     1081 ggttccgttt ctagctcccg agcggcgtta ccccagcgac gcggcgaacg tcaatctggc
     1141 aagatttttg catccctaga agcccctccc cttctgggga ggggttgggg tgggggctat
     1201 cgagatagcg taaggccgcg ggcccccacc cgctgcgact aagccagcaa gctggcaagt
     1261 ctcgctgccc tcccctgaag gggaggattt tctttctatc aatgcgccac gctctccccg
     1321 cgcacgatcc ccgacaaggc gagctgctca tcgatcgcgg cgagcaggcg tgccagcgcc
     1381 gcctcgtcct tcgcctctgc gcgcgcgacg agcacgtcct gcgtgttcga ggcgcgaagc
     1441 agccaccagc cgtcgtcggt cagcacgcgc gcgccgtcgg tgcggtcgac cttcgccccc
     1501 gtttcggcaa gccggtccag aacttcgtcc acaatggcga acttgcggct ctcgtcgacc
     1561 tggaagcgca tttcgggggt gttgaccatc gccggcattt cgctgcggag ctgcgtgacg
     1621 ctcttgccga gcttcgtcgc cgcctcgatc agccgcaccg cggcgtaagg cgcgtcgtca
     1681 tagccataat aacgatcggc gaaaaagata tgcccgctca tctcgcccgc cagcgggctg
     1741 ccggtttcct tcatcttcgc cttgatcagg ctgtggccgg tcttccacat cagcggtttg
     1801 ccgccaagct cggcgacgcg gtcgaacagc gcctggctcg ccttcacgtc ggcgatcacc
     1861 gtcgcaccgg gcatgtcctg cagcaccgcc gcggcataga tttgcagcag ctggtcgccc
     1921 cagatcaccc ggccctcgcc gtcgatcgcg ccgatgcggt cgccgtctcc atcgaaagcg
     1981 acgccgaaat cgaggctctt ctcggcgacg agcttcctga gatcggcaag attcttttcc
     2041 tcggtcggat cgggatggtg gttcgggaaa tggccgtcga tgtcggtaaa cagcaaatgg
     2101 tgctcgccgg gaaggcgcgc ggtcagcttc tcgatgacgg tgcccgcggc gccgttgccg
     2161 gcgtcccagc cgatgcggaa ggcgccgccc gcgaacccct cgaccagccg atcgacatag
     2221 gcgtcgacga tgtcgatgct ctgcgacgtg ccttctccgc tgtcccagtc gcccgcggcg
     2281 gccattttac cgatgccaag gatgtcggcg ccgtagaagg gacgcccctg catcacaaat
     2341 ttgaaaccat tatagtaggg ggggttgtgg ctgccggtta tctgtatgcc gccgtccaca
     2401 tcttcggttg acgcggcata atagagcatc ggcgtcggcc ccagcccgac gttcagcgca
     2461 tcgatccccg cggcattgat ccccgcgatc agcgcgtcgg cgagcatcgg cgacgacagc
     2521 cgtccgtcat aaccgaccgc gacgcgcttg ccgccggcgc ggcggatcag cgtcgcgaag
     2581 ctgcgtccga ttgcataggc gtccttgtcc gaaagcgtgt cgcccacgac cccgcggatg
     2641 tcatattcgc ggagcatcgt cggatggaaa ttgtgcgtca ttttttttac ctcttgggga
     2701 gagagggaac tcaggcggcg gcgaacaaat ccccatccgg atggaatcgc tcgcgcgccg
     2761 ggaggttcag tccgcccatc gcctcgcgga gttcctgccg cgcgacgacg tggccgattc
     2821 ccatgccgcc cagatgcgcc ttgtcgagca gggtgaggcc agccgggaaa agctcgcgat
     2881 agatgacgcg ctcgccgagc cccgggatga cgcggaaccc aacgcgccgc gacaattggg
     2941 tcaatgcctc gccgacgcgg cgcatattgt gcgcatcgac atgctggagg cggttgcgca
     3001 ggatgatcca gtcgatcgtc cgcccgtcgc tcttggcgcg cgccttgcgc gtgtcccaga
     3061 tgagttcgga atagaagctc ggccgggtga cctgaaaggt ttcggggtcg acctgtccga
     3121 tcaggtcgaa gtcgataaag ctgtcgttga tcggcgtgac gagcgtatcg gcgctggtcg
     3181 cgaggtgacg cgcaaagacg tcgtcgcgcc cgggggtgtc ggcgatcaga tagtcgacgt
     3241 cttcgctcag cgccgcgacc tgttcgtcga gttcctcgat cgtggagccg gtgaacacct
     3301 cgaagcgcgg ggtcggcagc gcgatctcgc ggcgcttcgc cgtattctcg cgattttcga
     3361 gatagcggtg aaaggtgcgc tggcgcgggt cgaggtcgat acccgcgacg cgccagccct
     3421 ggctcgccag cgcgaccgcg aaatgcacgg cgcaggtcga tttgcccgtc ccgccctttt
     3481 cattggcaaa gatgatgcgg tgcggtgcgg gcgtcgtcat gagcgttgcg attttcctgt
     3541 tggcacggca tgggcggccg tcgcgcgaag tgatatagtc gcgccaatat cggagggcga
     3601 cgagccgtgc aaatcatccg tgacatcgcg atgctgcacc gtgccgttac ggcactgaaa
     3661 caggggggga agagcgtcgc gctggttccg acgatgggct cgctccacga cggccatctc
     3721 tcgctcgtcc gcatgggccg gcgcgtcgcc gatcacgccg tcgtgtcgat cttcgtcaat
     3781 ccgacccagt tcggcccgaa cgaggatttc gacgcctatc cgcgcgacga ggcacgcgat
     3841 gccgcgatgc tcgtcgatga aggcgcgagc ctgctctggg cgcccgacgt cgcgaccatg
     3901 tatcccggcg ggcacagcac gcatatcgaa gtcgccgagc ttggcgccga ttattgcggc
     3961 gccgcacgcc ccggccattt cgacggcgtc gcgaccgtcg tcgccaaatt attcaatcag
     4021 gttcgccccg acgtcgcgat cttcggcgaa aaggactggc aacaactcgc gatcatccgc
     4081 cgcatggcgc gcgacctcga tttcggcatc gacattttgg gtgcgccgat cgcacgcgac
     4141 agcgacggtc tcgcgatgtc gtcgcgcaac gcctatctct cggacgcgca gcgcgcggcg
     4201 gcgcccgcct ttcccgccgc gctgaacgcc gcggcaaagg tgatcgcggg cggcgccgac
     4261 gtcgccgagg c
//
LOCUS       DATMSU010000265         3850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_80374, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000265 DATMSU010000000
VERSION     DATMSU010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80374"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VN034_07110"
     CDS             <1..466
                     /locus_tag="VN034_07110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HWV60403.1"
                     /translation="PIISSGPPSSPVFTAQQRALMTALSERVMPTTDTPGAIAAGVPE
                     YIEKLLADWASPDDRKPILAGLDAIEARSRADYKVAADKATPAQQDALLTLAMEDKLP
                     GGADFFEAFRQLVLTGYFTSEVGITQEREYLPVPGRYDGAYPYSEIKKVFSS"
     gene            463..1302
                     /locus_tag="VN034_07115"
     CDS             463..1302
                     /locus_tag="VN034_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWV60404.1"
                     /translation="MMIGRRQWMAGGAALAAALTGGPLMARSLKSKPIGIQLYTVREL
                     FSKDPMGTLEQVAAIGYREVEYGGGGYDKMDHAALRKTMDRLGLASPSIHVGYEALAS
                     DFDGAVAMTRALGADTLIVPYMGEAHRSAEGWKAAIADFNRYAERLKKAGLGFAYHNH
                     DFEFTLKPGGTSLFDTLIADADPALVQLELDLFWAVAAGEDPKAIIRRLPGRIYAYHV
                     KDRTADGKMTSVGKGVIDFADIFTLNEIAGVEHFYVENDQSPAPYLPDITTSFQTLSR
                     LRA"
     gene            1323..2999
                     /locus_tag="VN034_07120"
     CDS             1323..2999
                     /locus_tag="VN034_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWV60405.1"
                     /translation="MTDQFDAIVIGSGVSGGWAAKELTEKGLKVLMLDRGVMVEHGAD
                     YDYDGRPPYEIPAHNNMPEPLVKSDYFIATHGYVSPSNQKFYNNDRLNPYAYDEGEKF
                     YWIRPGAVGGKSLIWGRWSFRWSPEDFEANKRENVGIDWPIRYEDVAPWYDYVENYIG
                     VSGSRENLPQLPDSAFQPPIQMNVAEKWVKERLEATSPGRKLINTRLSNMTEDKEDQG
                     RSKCQFRNQCGRGCSFGAYFSTQAVTLPAARATGRLTLRSDAVVSNLEYDPKTKKVTG
                     VRVVDTKTKQAEVIPARLVFLCASAMASTQILMNSRIPGTAKSHFDASGTLGRYVMDH
                     IFRVGISGDIPGMTDFIEYGRRPGGVYVPRFRNVGGEEGVDFKRGYGYQGSARRDPLP
                     PEGFGASMKHGMRGYGPWKFGMGAFGECLPYEDNRVSLHADKVDRFGIPLMRFDVTFR
                     DNEIRMMDDARSEGEAMLRRAGLQNVTSGRSEHVPGDAIHEMGGARMAADPRQSVLNK
                     WGQAHDAANLFVTDGAQMASISCVNPSLTFMALTARAADYAVKQLKAGAI"
     gene            complement(3053..3373)
                     /gene="trxA"
                     /locus_tag="VN034_07125"
     CDS             complement(3053..3373)
                     /gene="trxA"
                     /locus_tag="VN034_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HWV60406.1"
                     /translation="MGTKAITDASFQADVIDSDTPVLVDFWAEWCGPCKMIGPALEEI
                     SDELAGKVVIAKLNIDDHPDAPSKYGVRGIPTMILFKNGAIADTKVGAAPKSALKGWL
                     EGAL"
     gene            complement(3437..>3850)
                     /locus_tag="VN034_07130"
     CDS             complement(3437..>3850)
                     /locus_tag="VN034_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HWV60407.1"
                     /translation="LGEAARAAMVDEVLAVLDDPAHAALFGPGSLAEVPLSAVVDGVV
                     VAGIVDRLLVTDDAVTVIDYKTGRHIPASAAEVAPAYLRQMAAYRDALRVIFPGRRVE
                     VALLYTAALRLITLDAALLDAHKPGLTATKANLPG"
BASE COUNT          714 a         1275 c         1233 g          628 t
ORIGIN      
        1 cccgatcatc agtagcggcc cgccttcgtc gccggtgttc accgcgcagc agcgcgcgct
       61 gatgaccgcg ctcagcgagc gcgtgatgcc gaccaccgac acccccggcg cgatcgcggc
      121 cggcgtcccc gaatatatcg agaagctgct cgccgattgg gcgtcgcccg acgaccgcaa
      181 gccgatcctc gccgggctcg acgcgatcga ggcgcgcagc cgggcggact ataaggtcgc
      241 cgccgacaag gcgacgcccg cgcagcagga cgcgctgctg acgctcgcga tggaggacaa
      301 gcttccgggc ggcgcggact ttttcgaggc tttccgccag ctcgtgctca ccggctattt
      361 cacgtcggaa gtcggcatca cccaggaacg cgaatatctc cccgtccccg ggcgctacga
      421 cggcgcctat ccctattccg agatcaagaa ggtgttttcg tcatgatgat cggcagacgg
      481 caatggatgg cgggcggcgc cgcgctcgcg gcggcgctga cgggaggacc gctgatggcg
      541 cgaagtttga agtccaagcc catcggcatc cagctctaca ccgtgcgcga gctcttctcg
      601 aaagacccga tggggacgct cgagcaggtg gcggcgatcg gttatcgcga ggtcgaatat
      661 ggcggtggcg gctatgacaa aatggatcat gccgcgctcc gcaagacgat ggaccggctc
      721 ggcctggcct cgccctcgat ccacgtcggc tatgaggcgc tcgcgagcga tttcgacggc
      781 gcggtcgcga tgaccagggc gctcggcgcc gacacgctga tcgttcccta tatgggcgaa
      841 gcgcaccgca gcgccgaagg ctggaaggcc gcgatcgccg atttcaaccg ctacgccgag
      901 cggctcaaaa aggcggggct cggcttcgcc tatcacaatc acgacttcga attcacgctg
      961 aagccgggcg gcaccagcct gttcgacacg ctgatcgccg acgccgatcc ggcgctcgtc
     1021 caactcgagc tcgacctgtt ctgggcggtc gccgcgggcg aggatccgaa ggcgatcatc
     1081 cggcgcctgc ccgggcgcat ctacgcctat catgtcaagg atcgcaccgc cgacggcaag
     1141 atgaccagcg tcggcaaggg cgtgatcgat ttcgccgaca tcttcacact caacgagatc
     1201 gcgggcgtcg agcatttcta tgtcgagaac gaccagtcgc ccgcgcccta cctgcccgac
     1261 atcacgacca gcttccagac gctcagccgc cttcgcgcct gaccgatatt cgaggacaag
     1321 atatgacaga ccagttcgat gcgatcgtca tcggctccgg cgtgagcggc ggatgggccg
     1381 cgaaggaact gaccgaaaag gggctcaagg tgctgatgct cgaccgcggg gtgatggtcg
     1441 aacatggcgc cgactatgat tatgacgggc ggccccccta tgaaatcccg gcgcacaaca
     1501 acatgcccga accgctggtc aagagcgact atttcatcgc cacccatggc tatgtctcgc
     1561 cgtcgaacca gaaattctac aacaacgacc ggctcaaccc ctatgcctat gacgagggcg
     1621 agaaattcta ctggatccgg cccggcgcgg tcggcggcaa gtcgctgatc tgggggcgct
     1681 ggagctttcg gtggagccct gaggatttcg aggcgaacaa gcgcgagaat gtcggaatcg
     1741 actggccgat ccgctatgag gatgtcgcgc cctggtatga ctatgtcgaa aattatatcg
     1801 gcgtgtcggg atcgcgcgag aatctgccgc agctccccga cagcgcgttc cagccgccga
     1861 tccagatgaa cgtcgccgaa aaatgggtga aggagcggct cgaggcgacg tcgccggggc
     1921 gcaagctgat caacacccgc ctgtcgaaca tgaccgagga caaggaggat cagggccgca
     1981 gcaaatgcca gttccgcaat cagtgcgggc gcggctgctc gttcggcgcc tatttctcga
     2041 cgcaggcggt cactttgccc gcggcgcgcg ccaccgggcg gctgacgctg cgctcggacg
     2101 cggtggtctc gaacctcgaa tatgatccga agaccaaaaa agtcaccggc gtgcgcgtgg
     2161 tcgacaccaa gacgaagcag gcggaggtga tccccgcgcg cctcgtattc ctctgcgcct
     2221 cggcgatggc ctcgacccag attttgatga actcgcgcat ccccggaacg gccaagagcc
     2281 atttcgacgc cagcgggaca ttgggccgct atgtcatgga ccatatcttc cgcgtcggca
     2341 tctcgggcga cattcccggg atgaccgact ttatcgaata tggccggcgt ccgggcggcg
     2401 tctatgtccc gcgcttccgc aatgtcggcg gcgaggaagg cgtcgacttc aagcgcggct
     2461 atggctatca gggcagcgcg cggcgcgacc cgctgccgcc cgagggcttc ggcgcgtcga
     2521 tgaagcacgg tatgcgcggc tatggtccgt ggaaattcgg catgggcgcg ttcggcgaat
     2581 gcctgcccta tgaggataat cgcgtcagcc tgcacgccga caaggtcgac cgcttcggca
     2641 tcccgctgat gcgcttcgac gtcaccttcc gcgacaacga gatccgcatg atggacgatg
     2701 cgcgcagcga gggcgaagcg atgctgcgcc gcgcgggtct gcagaacgtc accagcggac
     2761 gcagcgaaca tgtgcccggc gacgcgatcc acgaaatggg cggcgcgcgc atggcggccg
     2821 acccgcgcca gtcggtgctc aacaaatggg gccaggcgca cgacgccgcc aatctgttcg
     2881 tcaccgatgg cgcgcagatg gcctcgatct cgtgcgtcaa cccgtcgctc accttcatgg
     2941 cgctgaccgc gcgcgccgcc gactatgcgg tcaagcagct gaaggcgggg gcgatctaga
     3001 aatcccccta cgaccgtcat cccggcgaag gccgggatga cgggatcggg ccctacagcg
     3061 cgccttcgag ccagcccttg agcgcgcttt tcggcgccgc gccgaccttg gtgtcggcga
     3121 tcgcgccatt tttgaacagg atcatcgtcg ggatgccgcg cacgccatat ttgctcggcg
     3181 cgtcgggatg gtcgtcgatg ttgagcttgg cgatcaccac cttgcccgcg agttcgtccg
     3241 aaatttcctc gagcgccggg ccgatcatct tgcacggacc gcaccattcg gcccagaaat
     3301 cgacgagcac cggggtgtcg ctgtcgatga cgtcggcctg aaagctcgcg tcggtgatgg
     3361 ctttggtacc catagtcaaa actcctgaaa tatcgttggt gccaagctaa ggcgtcgcgg
     3421 cgtcccgctc aaggcctcaa cccggcaaat tcgccttggt tgccgtcaag cccggcttgt
     3481 gcgcgtcgag caaggcagcg tcgagggtga tcaaccgcag cgcggcggtg tagagcaggg
     3541 ccacctcgac ccgccgcccc ggaaagatca cgcgaagcgc gtcgcgatac gccgccatct
     3601 ggcgcagata ggcgggcgcg acctcggcgg cgctcgccgg gatatgccgc ccggtcttgt
     3661 agtcgatcac cgtcaccgca tcatcggtca ccagcaaccg gtcgacgatc cccgcgacga
     3721 cgacgccgtc gaccaccgcc gacagcggca cttcggcaag gctgccgggt ccgaacagcg
     3781 cggcgtgcgc cggatcgtcc agcaccgcca gcacctcatc gaccatcgcc gcgcgcgcgg
     3841 cctcgcccag
//
LOCUS       DATMSU010000266         7772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_175191,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000266 DATMSU010000000
VERSION     DATMSU010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7772
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175191"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1296
                     /locus_tag="VN034_07135"
     CDS             379..1296
                     /locus_tag="VN034_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWV60408.1"
                     /translation="MKAVFEISGTSRYDDLVTERYHFPKNYLAIARRCVGDWVIYRET
                     GASGGRKAYIAAAFVTAIDPDPADSCLFYARVDQFLPFDLAVPYRDREGRFAERFLRD
                     YDDPATVGRLMRGRSVRLLDGADFVAIVDAGLNAVLDAENAIRLELDTPHLDPATAAL
                     LSEPPSERRVEQMLVNRKIRDAAFRKNVLDAYDSTCAVTGLRIVNGGGKAEAQAAHIW
                     SVASGGPDVVQNGIALSATAHWLFDRHLISLADDYSLLISHNKVPTELRGLFPPMGQT
                     VRLPKDQHSWPRPDYMARHRERFAGYGLV"
     gene            1293..3032
                     /locus_tag="VN034_07140"
     CDS             1293..3032
                     /locus_tag="VN034_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWV60409.1"
                     /translation="MSSTASIQIYDAQAKELAERYDRLSLLESYGGIEELIRPSHPAP
                     LALDVGAGSGRDAAWLLSLGYEVVAVEPAAGMRAEGERRHPDPKIHWLDDRMPGLAAV
                     HSTAISFDLILLSAVWQHIAPSDRARSFRKLVTLLKPGGLLLMTLREGPSPPDRPMYG
                     VSLGEVEGLARAHGVEVLRVIEKADAMARGDVRWTMVTLRMPDDGAGALPLLRGIILA
                     DDKSSTYKLGLLRAIARIAEHAPAAARAADDENDVIDVPLGLVALFWIRMYLPLVRLG
                     FPQAPKNRGVDGLGFAKAGFRALLEEAIAPQELRVGSAFAADRAVMISRALGEAVSTI
                     ATMPANFIRFPNSDQRVFAAFKARSRTGLSELQIDLPTLAGWGQLRIPGAIWRTMARL
                     GSWIEPVLVNEWARLMRGYAERMGVSIPVGQAEAALVWEEPFRDTALGRSAAQRIFAE
                     GRDIYCAWSATKLRPSRLDIDHCMPWSAWPCGDLWNLLPSERRVNQHQKRERLPSGEL
                     LLNSRERIIGWWSDAWLSSPTLSQRFWREASAALFVAEPSEPEQLFAGLEWRRLRLRQ
                     DQLVPEWTGEQRV"
     gene            complement(3551..4294)
                     /locus_tag="VN034_07145"
     CDS             complement(3551..4294)
                     /locus_tag="VN034_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWV60410.1"
                     /translation="MRFGLGSPYLDIRLPCGGGIDLLFTPRPDRAAIEAAIALLDRRK
                     PAAFALAPTGVSFTDVLAPTGWRGNDFVVTYVPQLRILAFGQGEELTAAAALAAASGA
                     ITAAFSPYTRDIAALRAAKVDAELLPYRSWRPGMAFDPWTAFLFLFHDRDWEEELIPW
                     ALEQSRFYIGALGSRRTHADRKTVLEAAGVAAEAIASIRSPVGLVPSTRDPATLAISV
                     LAEIVEQFAADSFLPLTKTRREQVLRLDR"
     gene            complement(4295..4537)
                     /locus_tag="VN034_07150"
                     /pseudo
     CDS             complement(4295..4537)
                     /locus_tag="VN034_07150"
                     /inference="COORDINATES: protein motif:HMM:NF014662.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
     gene            4589..5155
                     /locus_tag="VN034_07155"
     CDS             4589..5155
                     /locus_tag="VN034_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWV60411.1"
                     /translation="MIEANRTAAILLAAGRSERFGSDKLLALVRDEPIAVLAAKSLLA
                     IEFGRLIAVCRAGSDVAAILSDLGFEIVTNPDPARGLSSSLALGIERAGELNVDAALV
                     ALGDMPFVGPQHTKSLLVAFDPAKASIVASGRDGTAMPPALFARSHFDALRTIEGDRG
                     GRALLAGALLVAGDPLELADIDRPEDLR"
     gene            complement(5169..7376)
                     /gene="paoC"
                     /locus_tag="VN034_07160"
     CDS             complement(5169..7376)
                     /gene="paoC"
                     /locus_tag="VN034_07160"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HWV60412.1"
                     /translation="MKFDTPATDNPIDRLKVVGKPTNRIDGPLKTSGTAPYAYERHDV
                     VPNQAYGYVVGSAIAKGRIASIDTGAAKAAPGVLAVVTAANAGKLGKGDFNTARLLGG
                     PDIEHYHQAVALVVAESFEQARAAAALVRVQYDRAKGNFDLAKSMDGAVKPPPAFGTE
                     ADTRFGDFDAAFASAPIKLDARYTTPDHSHAMMEPHASIASWDGDRLTLWTSNQMIAW
                     SAGDMAKTLGIPKENVRLVSPYIGGGFGGKLFIRADALLAALGAKAAGRPVKVALQRP
                     LMTNNTTHRPATIQRIRLGATTDGKLTAIGHESWSGDLKGGSPETAVAQTRLLYAAPN
                     RMTAMRLATLDLPEGNAMRAPGEAPGMMALEIAIDEMAEKLGADPIAFRILNDTQVDP
                     EKPERPFSQRRLKECLELGAKTFGWKDRNPKPGATRDGRWLVGMGVAAAFRNNLNEKS
                     AARVRLDRHGMVTVETDMTDIGTGSYTIIAQTAAEMMGVPLDRIDVRLGDSSFPVSAG
                     SGGQWGANSSTAGVYAACAKLRAAIAEKLGLDPAAAVFENGKVRAGGKSVALAKAAAD
                     GDLVAEDMMEYGDLAQKYQQSTFGAHFCEVGVDAMTGEIRVRRMLAVCAAGRILNPKA
                     ARSQVIGAMTMGAGAALMEELAVDKRFGFFVNHDLAGYEVPVHADIPHQEVIFLDEVD
                     PTTSPMKAKGVGELGICGVAAAVANAVYHATGVRVRNYPVTLDKLIDKLPDLA"
     gene            complement(7381..>7772)
                     /locus_tag="VN034_07165"
     CDS             complement(7381..>7772)
                     /locus_tag="VN034_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWV60413.1"
                     /translation="IALADFYRAPGSTPHLETVLAPGELITAVTLPKPLGGTHIYHKV
                     RDRASYAFALISVGAVIQRDGSGRVALGGVGYKPWRVEAADAELPRGAKAAASALLAG
                     ANTSHENAYKLPLVERTLAAALMQAKG"
BASE COUNT         1338 a         2357 c         2534 g         1543 t
ORIGIN      
        1 gacccacaga agttggccag cgcttcctca cggggtggcg gagaaacctt gatcgggcca
       61 tcagggctgc ttctgtgcac tgtgttccaa aagccgcctg gccgcaaagc ggttcaagag
      121 cggacggtcc ctttgcggcc aaacaccgtg ttgacgctct tcgcgcaact gcgatgtcga
      181 tgtacgcagt gaagctacgc tagctcaatc tacatttcat catttcatct ctgcgacatt
      241 tttggatgat cgcgcgcggc gatgcttgac ggacctgggt ttcgatcggc ttagtggcaa
      301 tgggcggaga ttgtcgcggg gacggaataa tatgagcctt acaaatcctg ggtttggctg
      361 ccgagcaggt tcgcgcgctt gaaagcggtc tttgagatca gcggaacatc ccgctatgat
      421 gacctcgtta cggagcggta tcatttcccg aaaaactatc ttgcgatagc gcggcgatgc
      481 gtaggcgact gggtcatcta tcgcgaaacc ggggcatcgg gcgggcgtaa ggcctatatc
      541 gcagcagcgt ttgtgaccgc aattgatccc gatccggcag acagctgttt gttctacgcg
      601 cgtgtcgacc agtttcttcc gtttgacttg gcggttccat accgtgatcg cgaagggcgg
      661 ttcgccgagc gctttttgcg ggactatgac gatccagcta cggtcggcag acttatgcgg
      721 ggcaggtccg ttcggttgct ggatggtgcc gatttcgttg cgatcgtcga tgcgggcttg
      781 aacgctgttc tcgacgctga gaatgccatc cgccttgaac tggatacccc gcatctcgat
      841 ccggcaacgg cggcgctttt gagcgagccg ccgagcgagc ggcgcgtcga gcaaatgctc
      901 gtgaaccgca agattcgcga cgcggcattt cgaaagaatg tgctcgatgc ttacgacagt
      961 acttgtgccg tgaccggcct gcggatcgtc aacgggggtg gcaaagcgga agcgcaggcc
     1021 gctcatatat ggtcggttgc cagtggcggg cctgatgtcg tgcagaatgg aatcgcgctt
     1081 tcggcgacgg cccattggct gttcgatcgc catttgatca gtctcgctga cgactattct
     1141 ttgctgattt cgcataacaa agttccaacg gagcttaggg ggctttttcc gccgatgggc
     1201 cagaccgtcc gcttacccaa agatcagcat agctggccac gtcctgacta tatggcacga
     1261 catcgtgagc ggtttgcggg atatgggctc gtgtgagcag caccgcctca atccaaattt
     1321 atgacgcgca ggcgaaagaa ttagcggagc gctatgatcg cctatcgctt ctcgaatcct
     1381 atggcggcat cgaagaattg atccggccat ctcatccggc gccgcttgcg ctcgatgtcg
     1441 gagccggctc aggcagagat gccgcttggc tgttaagtct gggatatgag gtcgtcgcgg
     1501 ttgaaccggc ggcgggcatg cgcgcggaag gcgagcgtcg tcaccccgat cccaagattc
     1561 attggctcga cgatcgaatg cctggactcg ctgcggtaca ttcgacggcg atcagcttcg
     1621 atctcatcct gctttccgcc gtttggcagc atatagcgcc gagcgaccgt gctcgtagct
     1681 ttcggaagct cgtcaccctg ctcaaacccg gtggccttct tttgatgacg ctgcgagagg
     1741 gaccatctcc tcctgatcga ccgatgtacg gggtttcgct tggcgaggtc gaaggcctcg
     1801 cacgcgcgca tggtgtcgaa gtgctgcgcg tcatcgaaaa ggccgacgcg atggcccgcg
     1861 gtgacgtccg ctggaccatg gtgaccttgc gcatgcccga tgatggcgcc ggcgcgcttc
     1921 ctttgctccg cgggatcatt ctcgcggatg acaaatcgtc gacatacaaa ctcggcttgc
     1981 tccgggccat tgcacgtatt gcggaacatg cgcccgccgc ggcccgagca gcagacgatg
     2041 agaatgacgt catcgatgtt ccacttggcc tagtcgcgtt gttctggatt cggatgtacc
     2101 tgccgctggt aaggctggga ttcccgcaag ctccgaagaa ccggggtgtc gatggcctcg
     2161 gctttgcgaa ggcgggtttc agggcgttgc tggaggaggc gattgcgcct caggaattgc
     2221 gtgtcggttc cgctttcgct gccgatcggg ctgtcatgat atcccgcgct cttggggaag
     2281 ctgtctcgac gattgctacg atgccagcaa atttcatccg ctttcctaat tctgaccagc
     2341 gcgtctttgc agctttcaag gcgaggagcc gcaccggatt gagtgagctg caaatcgatc
     2401 tgcccacttt ggcaggctgg ggccagttgc gcattcccgg tgccatatgg cgaacgatgg
     2461 ctcggcttgg atcttggatc gagcccgtgc tcgtgaacga atgggcgcgt ctcatgcgcg
     2521 gttatgcgga acgcatgggg gtttcgatcc cggtcgggca ggcggaggca gcgctggttt
     2581 gggaggagcc tttccgcgat accgcgctcg gccgatcggc tgcccagaga atatttgccg
     2641 agggccggga catctattgt gcgtggtccg ccacgaaact gaggccatcg cgactggata
     2701 tcgatcattg tatgccgtgg agcgcctggc cgtgtgggga cctttggaat ctcctcccga
     2761 gcgagcgtag ggtcaaccaa catcagaagc gggagcgcct tccttccggc gagttattgc
     2821 tcaactctcg cgagcgcatc attggttggt ggagcgacgc atggctttcg agcccgacat
     2881 tgtcacagcg cttttggcgc gaggcgtctg ctgcgctctt tgtcgcagaa ccaagtgagc
     2941 ctgagcaact cttcgctggt ctggaatggc gacgacttcg tcttcgacag gatcagctcg
     3001 tacccgagtg gaccggagag caacgcgttt gacatgcgtg ctcgcgctgc gccgatttcg
     3061 acggcattgc agaggaggcg ccagaatatc acccgatgat tttcgcaata aaagcattgt
     3121 gtggagcaac tacagacggt ataagattta ttgagatatc gtgatggtaa ttggtaatcg
     3181 tatcactgca tctctgaatt tatagtgtca ccggtggggc catcatatat tggtataata
     3241 attagatttt tcttatcaaa ttcataggag tattcttagt ttcctcttct ggcaccattc
     3301 ccggtcaaaa ctgggcacca ccccgccggg gtggcggtca ccccgaacga gggcatgctc
     3361 tagcgcgagt ttcgagccgt agatcgtgat atggtcgttg ccgaagccaa ccttgccgac
     3421 gatgaggcgg acgtaggcgt cggagcaaag cgactatggc gtccgtctgc gcgcaagatg
     3481 ggatttctga taagagacgg atctgcagtg ggtggctgag acagcccggc cattcccacc
     3541 ggcaagagag ctatcgatcc aatctcaaaa cttgctcgcg gcgcgttttt gtcaagggga
     3601 gaaacgagtc ggcggcaaac tgctcgacga tttcggcgag caccgaaata gcaagggtcg
     3661 cgggatcgcg cgttgacggg acgaggccta ccggcgatcg gatcgacgcg atggcttcgg
     3721 ccgccacgcc ggcggcttcg agcacggtct ttcgatcggc gtgcgtccgg cggctgccta
     3781 aagcgccgat gtagaagcgc gactgttcca aggcccaggg tatcagctct tcctcccagt
     3841 cccggtcgtg gaaaaggaaa agaaatgcgg tccaggggtc gaaggccatg ccgggacgcc
     3901 agcttcgata gggaagaagc tccgcatcga ccttggccgc gcgaagcgcc gctatatcgc
     3961 gcgtgtaggg cgagaaggcg gccgtgatgg cgcccgaggc cgccgccagc gcagccgccg
     4021 ccgtcaactc ttcgccttgg ccgaacgcca ggatgcgaag ctgcggtaca taggtgacga
     4081 cgaagtcgtt gccccgccag ccggtcggcg cgagcacgtc ggtgaagctg acccccgttg
     4141 gcgcaagcgc aaacgcggct ggtttacgcc tgtcgaggag cgcgatcgcg gcttcgatcg
     4201 ccgcacgatc gggccgcggg gtgaacaaaa ggtcgattcc cccgccacac ggcagccgga
     4261 tgtcgagata gggcgagccg aggccgaacc gcacgaggcg cggggcaccc gctgcgagag
     4321 cgtcgagggc ttcggcgacg acggcggcct cgatgcagcc gctcgagagc gagccaacat
     4381 aatcgccgtt cgccgcgatg gccatctgcg cgcctagggc gcggggagac gccccctcga
     4441 tgccggtaag cgtcacgagg acgcaggcgt cgccggactc gctcctgcgg cgcagaaagt
     4501 gcaatatgtc gagcgaagac gttgccaagc cggtcattac gatcgccctg ccgaattgcc
     4561 tggccgctgc gcaacgggag acaatgccgt gatcgaagcc aatcgcaccg ccgccattct
     4621 gcttgcggcc ggccgttccg agcgcttcgg cagcgacaag ctgcttgcgc tggttcgcga
     4681 cgaaccgatc gcggtccttg cggcgaaaag cctgctcgca attgagttcg gccgcctgat
     4741 cgcggtctgc cgcgcgggaa gcgacgtcgc cgcgattctg tcggacctcg ggttcgaaat
     4801 cgtcaccaac cccgaccccg cgcgcggact atcttcctcg ctagctctcg ggatcgaacg
     4861 ggccggggag ttgaatgtcg acgcggcgct ggtggcgctc ggcgacatgc cgttcgtcgg
     4921 accgcagcat accaaatcgc tgctggtggc gttcgacccc gccaaggcgt cgatcgtcgc
     4981 ctcggggcgt gacggcaccg ccatgccgcc ggcgcttttc gcacgttcgc acttcgatgc
     5041 gcttcgtacc atcgaaggcg accgcggggg ccgcgcattg ctcgccggcg ccctgctcgt
     5101 cgccggggat ccgctcgagc ttgccgacat cgaccgtccc gaagacctgc gctgagcgag
     5161 gctggtcgtc acgcgaggtc cggcagcttg tcgatcagct tgtcgagggt gaccgggtaa
     5221 ttccgcaccc gaacgcccgt tgcatgatag acggcgttgg cgaccgcggc cgcgacgccg
     5281 cagatgccga gttcaccgac ccccttggct ttcatcggcg acgtcgtcgg atcgacctcg
     5341 tcgaggaaga tcacctcctg gtgcggaatg tcggcatgga cggggacttc atagcccgcg
     5401 aggtcatgat tgacgaagaa gccgaacctc ttgtcgaccg ccagctcctc catcagcgcc
     5461 gcgcccgcgc ccatcgtcat cgcgccgatg acctggctgc gcgcggcctt ggggttgagg
     5521 atgcgcccgg cggcgcagac cgcgagcata cggcgcaccc gtatctcgcc cgtcatcgcg
     5581 tccaccccga cctcgcagaa atgcgcgccg aaggtcgact gctgatattt ctgggcgagg
     5641 tcgccatatt ccatcatgtc ctcggcgacg agatcgccat cggccgcggc cttcgcgagc
     5701 gcgacgctct tgcccccggc gcggaccttg ccgttttcga agactgcggc cgcggggtcg
     5761 agaccgagct tctccgcgat ggccgcgcgt agcttggcac atgcggcata gacacccgcg
     5821 gtcgaactgt tcgctcccca ctggccgccc gaacccgccg agacggggaa ggacgaatcg
     5881 ccgagccgca cgtcgatgcg gtcgagcggc acacccatca tctccgctgc ggtctgcgcg
     5941 atgatcgtat agctgccggt tccgatgtcg gtcatgtcgg tttcgaccgt caccatgccg
     6001 tgtcggtcga gcctcacgcg cgccgccgat ttttcgttga gattgttgcg gaaggccgcc
     6061 gcgacgccca tgccgacgag ccagcgcccg tcgcgcgtgg cgcccggttt cgggttgcgg
     6121 tccttccagc cgaaggtctt ggcacccaat tcgagacatt ccttgagacg gcgctgcgag
     6181 aaggggcgct cgggcttttc gggatcgacc tgcgtgtcgt tgaggatacg gaaggcgatc
     6241 ggatcggcgc cgagcttctc cgccatttca tcgatcgcga tttcgagcgc catcatcccc
     6301 ggtgcttcgc cgggcgcgcg catcgcattg ccctcgggaa ggtcgagcgt cgcgaggcgc
     6361 atcgcggtca tccggttggg cgcggcgtaa agcagccggg tttgcgcgac cgcggtttcg
     6421 ggactgccgc ccttgaggtc gcccgaccag ctttcgtggc cgatcgcggt gagcttgccg
     6481 tcggtcgtcg cgccgaggcg aatgcgctgg atcgtcgccg gccggtgcgt cgtgttgttg
     6541 gtcatcagcg gccgctgcag cgccactttc accggacgac ccgcggcttt ggcaccgagc
     6601 gcggcaagca acgcgtcggc gcggatgaac aattttccgc cgaaaccgcc accgatgtag
     6661 ggcgagacca gccggacatt ctctttgggg atgcccagcg tcttcgccat gtcgcccgcc
     6721 gaccaggcga tcatctggtt cgacgtccac agcgtcagcc tgtcgccgtc ccaactcgcg
     6781 atcgacgcgt gcggctccat catcgcatgg ctgtgatcgg gcgtcgtgta gcgtgcgtca
     6841 agcttgatcg gcgcgctcgc gaaggccgcg tcgaaatcgc cgaagcgcgt gtccgcctcg
     6901 gtgccgaagg ccggtggcgg tttgaccgcg ccgtccatcg atttcgcgag gtcgaaattg
     6961 cccttcgcgc gatcatattg gacgcgaacg agcgcggcgg ccgcgcgcgc ctgctcgaaa
     7021 ctttcggcca cgaccagcgc gaccgcctgg tgataatgtt cgatgtcggg gccgccgagc
     7081 aggcgggcgg tgttgaagtc gcccttgccg agcttcccgg cgttcgcggc ggtgacgacg
     7141 gcgaggacgc cgggcgcggc ttttgcggca ccggtatcta tcgaggcgat gcggcccttg
     7201 gcgatcgccg agccgacgac atagccatag gcctggttcg gcacgacatc gtgccgctca
     7261 taagcatagg gcgcggtgcc gctggtcttg agcggaccgt cgatgcggtt cgtgggcttg
     7321 ccgacgacct tcagccggtc gatcggattg tcggttgcgg gcgtgtcgaa cttcatgggc
     7381 tcaacccttc gcttgcatca gcgccgcggc gagcgttcgc tcgaccagcg gcagcttata
     7441 ggcattttcg tgactcgtat tggcgccggc caggagcgcc gaagcggcgg ccttggcgcc
     7501 gcgcggcaat tcggcatcgg ccgcctcgac gcgccagggc ttatatccga ccccgccgag
     7561 cgcgacgcgt ccgctgccgt cgcgctggat caccgcgccg accgagatca gcgcgaaggc
     7621 ataagaggcg cggtcgcgga ccttgtggta gatatgggtg ccgccgagcg gcttgggcag
     7681 cgtgaccgcg gtgatgagtt cgcccggcgc gagcaccgtt tcgagatgcg gtgtgctgcc
     7741 gggcgcgcga tagaaatcgg cgagcgcgat gg
//
LOCUS       DATMSU010000267         6057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_14173, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000267 DATMSU010000000
VERSION     DATMSU010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14173"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VN034_07170"
     CDS             complement(<1..1492)
                     /locus_tag="VN034_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV60414.1"
                     /translation="MSYSPPRRPHRFARSLFLSGAAALLLAPLAASAQNDGGAATARS
                     QAKNADWLYAGSDIPRDTAWQFGVLPNGLRYAVRNNGVPPGQVSIRVRMDVGSMFETE
                     EERGYAHLLEHLTFRGSEHIPDGEAKRIWQRFGVTFGSDSNAQTTPTQTVYQLDLPSV
                     TPANLDEGMKLLSGMIRAPRISELAVAAERGVVTAELRESNGPQKRIADATNAHLFAG
                     QLLGDRSPIGTTESLGKASATTVGAFHDRWYRPDRAVVVISGDGDPAEFARLIAKYYG
                     DWQADGPNPATPDFGKPDPNQPAALEIVEASQPLALTLAMVRPWKKRIDTVANTRRLY
                     LEYLAQALVNRRLENTARAGGSYLVATVEQQYVSRSADVTMASIVPLSDWKAALADVR
                     GVIADAVKTPPSQADIDREMNEIEAFLLKELENARNEPGARLADDMVRAVDIGETVTS
                     PQGQVDMFKAIRASATPQVMLDISKAIFAAPVTRVVLTTPTTAGGNA"
     gene            1577..2437
                     /locus_tag="VN034_07175"
     CDS             1577..2437
                     /locus_tag="VN034_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HWV60415.1"
                     /translation="MNASPPAPLTALIQMTSGIDPDANLATIDRAIADAAARGAAMAF
                     LPEMSLLLDRDRARSGGHIAREADSHWPAALQEMAQKHGIWLHSGSMPLLADDGERRV
                     NRSHVIAADGSIRARYDKVHMFDVALPSGENWRESATYVGGDSIAVVDTPLGRMGLSI
                     CYDIRFPELYRTLAERGATIIAIPAAFTVSTGEAHWHVLMRARAIETQCHVVAAAQCG
                     THADDRQTYGHSVAIDPWGAILADAGGEEAMVEKGYALTLAPVDAAAVERARRAIPLA
                     GSRTNRTLTL"
     gene            complement(2542..2772)
                     /locus_tag="VN034_07180"
     CDS             complement(2542..2772)
                     /locus_tag="VN034_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60416.1"
                     /translation="MNLLKKAAISLAATSMIAAPVAASAAPAARAASAVDGQSELEGS
                     TSWIIAVLALVAVVGGIIIASDNDDDEPVSPG"
     gene            complement(2948..3745)
                     /locus_tag="VN034_07185"
     CDS             complement(2948..3745)
                     /locus_tag="VN034_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWV60417.1"
                     /translation="MTDAASPTAPPEVLPEGAIPAATLVIMRPSDSGGPDEILMVKRS
                     ATMAFAAGAVVFPGGRVDPDDYRVARLHGFGVDEADGAARVAALRETLEETGLAVGWP
                     GLAEKEMAEVRRALLGGTLLSDILAARGDRIALESFVPFARWCPNFKEARTFDTRFYA
                     VAAPPHSHELTVEEAEHSHIFWASAEATLAMADRGELSVIFPTRRNLERIAQASDFSG
                     FSVHSRRFPVELVTPWIEDRDGQSYLCIPTHLGYPVTSEAFDRVRRG"
     gene            3856..4482
                     /locus_tag="VN034_07190"
     CDS             3856..4482
                     /locus_tag="VN034_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HWV60418.1"
                     /translation="MGIVEILGVAGSLSLLAGWRLYLTILATGVAMRFGWLPLPEHLV
                     ALQILANPWVLGVALVGTLAEFLADKIAWVDSAWDTVHSIVRPLGGALLALALVDSSD
                     PAWQVIAFLLGGGGALLSHGAKATTRAVVNVSPEPFSNAAVSTGEDVATGGLLALAIA
                     YPPLAIVIAVLLAVAAVVVIIALRRLLRNIKATLKRALGDEPPPAIDA"
     gene            4556..>6057
                     /locus_tag="VN034_07195"
     CDS             4556..>6057
                     /locus_tag="VN034_07195"
                     /inference="COORDINATES: protein motif:HMM:NF039177.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60419.1"
                     /translation="MSPKYSLRPILLSSVAALALGQASPTLAQCVFTPTADDDVHICD
                     TGTSVGGLDDPDGNNQLTLPVGGTGTVAGDVSFGGGADIVVIDSGRIEGAVDQGNGND
                     SFSISAGTVTGQVQQGEGIDDFAMTGGEIDRLNQGGSMDTFLMTGGRIVDAFDDGDTA
                     RMTGGRIGRVNMKLADNLFDMSGGTIDRNLVAGFGNDTIILSAGTIGGNISVSGGTDS
                     VTITGGSVGGDVLMSVGTDSFVWDGGGVVHGTVDLGGDDDSARLANLTNANLGGSPRI
                     TGGAGSDALVFDNVTTDGVARFDGWENIQATNDSELTFDGLLTLGDAGTGTGRFELDA
                     SSTIFGGGQRGGIVAFTAGERATLINAGRIDLTNGGNVPGDSFTVGGDYVGNDGLLLI
                     DTVLGDDVSLSDKLVIDGGSASGTTGITVLNAGGTGALTQQNGILVVEAVGGATTASG
                     AFALNNRLAVGAYEYFLFRGGTTTGTDENWYLRSTLPPPPEPTPEPETPPP"
BASE COUNT         1022 a         2012 c         2005 g         1018 t
ORIGIN      
        1 cggcattgcc gcccgccgtc gtcggcgtcg tcagcacgac gcgcgtgacc ggcgcggcga
       61 agatcgcctt gctgatgtcg agcatcacct gcggcgtcgc cgaggcgcgg atcgccttga
      121 acatgtcgac ctgtccctgc gggctcgtca cggtttcgcc gatgtcgacc gcgcgcacca
      181 tgtcgtcggc gagccgcgcg ccgggttcgt tgcgggcgtt ctccagctcc ttcagcagaa
      241 acgcctcgat ctcgttcatc tcgcggtcga tgtcggcctg cgacggcggt gttttcacgg
      301 catcggcgat caccccgcgc acatcggcga gcgcggcctt ccagtcgctc aggggaacga
      361 tcgacgccat cgtcacgtcg gcgctgcggc tgacatactg ctgttcgacc gtcgcgacga
      421 gatagctgcc cccggcgcgc gctgtgtttt cgaggcggcg gttgacgagc gcctgggcaa
      481 gatattcgag gtagagccgc cgcgtgttcg cgaccgtgtc gatacgcttc ttccacggcc
      541 gcaccattgc gagagtgagc gcgagcggct ggctggcctc gacaatctcg agcgcagcgg
      601 gctggttcgg gtcgggtttg ccgaaatcgg gcgttgcggg attcgggccg tccgcttgcc
      661 aatcgccgta atatttggcg atcaggcggg cgaattcggc cgggtcgccg tcgcccgaga
      721 tgacgaccac cgcccggtcg gggcgatacc agcgatcgtg aaaggcgccg accgttgtcg
      781 cgctcgcctt gccaagcgac tccgtggtgc cgatcggcga gcgatcgccg agcagctggc
      841 cggcaaaaag atgcgcgttg gtcgcgtcgg cgatccgctt ttgcggaccg ttggattcgc
      901 ggagttccgc cgttaccaca ccgcgttcgg cggcgaccgc gagttcggaa atgcgcgggg
      961 cgcggatcat gcccgacagc agtttcatgc cttcgtcgag attagccggc gtgacgctgg
     1021 gcaggtcgag ctggtagacc gtctgcgtcg gcgtcgtctg cgcgttcgaa tcgctgccga
     1081 aggtcacgcc gaagcgctgc cagatgcgct tcgcctcgcc gtcggggata tgttccgacc
     1141 cgcggaaggt cagatgctcg agcaaatgcg catagccgcg ctcttcctcg gtttcgaaca
     1201 tcgagcccac gtccatgcgc acgcggatcg acacctggcc cggcggcacg ccattgttgc
     1261 gcaccgcata gcgcaggccg ttggggagca ccccgaactg ccacgcggta tcgcgcggaa
     1321 tgtcgctgcc ggcatagagc cagtcggcgt tcttcgcctg gctccgcgcc gtcgccgccc
     1381 caccatcgtt ctgcgcgctc gcggcaagcg gcgccagaag cagtgcggcg gcaccggaca
     1441 ggaaaagcga acgggcgaag cgatgcgggc ggcgcggggg agaataggac attgacctat
     1501 cttatcgctt ccgaaccttg ctggccactg aactgtcatg cttgcccgga gcgcggcacg
     1561 cgcttagcca gaaacgatga acgcttcccc tcctgccccg ctgaccgcgc tgatccagat
     1621 gacgagcggc atcgaccccg acgcgaatct ggcgacgatc gatcgcgcca tcgccgacgc
     1681 cgcggcgcgc ggcgcggcca tggccttcct ccccgaaatg tcgctgttgc tcgatcgcga
     1741 ccgcgcgcgg tcgggcggcc atatcgcccg cgaagccgac agccactggc ctgccgcgct
     1801 gcaggaaatg gcgcagaaac acgggatctg gctgcattcg ggatcgatgc ccttgctcgc
     1861 cgacgacggc gagcggcgcg tcaaccgcag ccacgttatc gctgcggacg gcagcatccg
     1921 ggcgcgctat gacaaggtcc acatgttcga cgtcgctctg ccctcgggcg aaaactggcg
     1981 ggagtcggcg acttatgtgg gcggggattc gatcgcggtg gtcgacacgc cgctcggacg
     2041 gatgggcctc agcatctgct atgacatcag gtttcccgaa ctctatcgga cgctggccga
     2101 acgcggggcg acgatcatcg cgatccccgc cgccttcacc gtatcgaccg gagaggcgca
     2161 ttggcatgtc ctgatgcgcg cgcgagcgat cgagacgcaa tgccatgtcg tggcagcggc
     2221 acaatgcggc acgcacgccg acgacaggca gacctatggc cacagcgtcg cgatcgatcc
     2281 ctggggcgcg atccttgccg atgcgggggg agaggaggcg atggtcgaga agggttatgc
     2341 gttgacgctg gcacccgtcg acgcggcagc cgtcgaacgc gcccgtcggg cgatcccact
     2401 cgccgggtca cgaacgaacc ggaccctcac gctctgattt tcacaagaca cagtggggcg
     2461 cgtacaaatt tcgcggcccg ggataaacaa agggctccga gcatagctcg gagccctttg
     2521 ggaaactctg gactagccag ctcagcccgg gctgaccggt tcgtcgtcgt cgttgtccga
     2581 cgcgatgatg atgccgccaa cgacagcaac gagagcgagg acagcgatga tccagctcgt
     2641 gctgccttcg agttcgctct ggccgtcaac cgccgaagct gcgcgagccg cgggggctgc
     2701 cgatgcggcg accggagccg cgatcatcga ggtcgctgcg agagagatcg cagccttctt
     2761 aagcaggttc atcttattct ccgtccactg gattactctt gtcgaatcac accaggggaa
     2821 cctccccggt ctgcttctgc agcgccttct taggcacggc cttcagcaat tgcaacgacg
     2881 ttttgcccta gcccgccaaa tgttagtaaa taataaatag agaaaaagtt gtcccagaga
     2941 acagcggtta tccgcgccgc acgcggtcga aggcttcgct cgtgacgggg tagccgagat
     3001 gggtcgggat gcacagatag ctctgcccgt cgcggtcctc gatccacggc gtgacgagtt
     3061 cgaccggaaa gcgccggcta tggacagaga aacccgaaaa gtcagatgct tgcgcgatgc
     3121 gttctagatt gcgccgggtc gggaagatca ccgacagctc gccgcgatcg gccatcgcca
     3181 gcgtcgcctc ggcgctcgcc cagaagatat ggctgtgctc ggcctcttcg accgtcagtt
     3241 catggctgtg cggcggggcg gcgacggcat aaaatcgcgt gtcgaacgtc cgggcttcct
     3301 tgaagttcgg gcaccagcgc gcgaaaggca cgaagctttc cagcgcgatt cgatcgccgc
     3361 gcgcggccaa tatgtcggaa agcagcgtgc cgccgagcag cgcccgacgc acctccgcca
     3421 tctctttttc cgccaacccc ggccagccga ccgcgagccc ggtttcctcg agcgtctcgc
     3481 gcagcgcggc gacacgcgcc gcgccgtcgg cctcgtcgac cccgaagcca tggaggcgcg
     3541 cgacgcgata atcgtcggga tcgacccgcc ccccgggaaa gacgaccgcg cccgccgcga
     3601 acgccatggt cgccgaccgt ttgaccatca atatctcgtc gggcccgccg ctgtccgacg
     3661 ggcgcatgat gaccagcgtc gccgccggaa tggcgccttc gggcaagact tcgggcggtg
     3721 cggtcggcga agctgcatcg gtcatgaccc gagcgataac cgggccacgc cagcttgtca
     3781 tcccggcatt gcgggcgcgc gcggacgggt gcaagatgat gcacgatgac gcgatccgga
     3841 acggggagca ggatattggg aatcgtcgag attttgggcg tcgcgggcag cctgagcctc
     3901 ctcgccggct ggcggctcta cctcaccata ttggcgaccg gcgtggcgat gcggttcggc
     3961 tggctgccgc tccccgagca tctcgtggcg ctgcagatac tcgccaatcc gtgggtgctg
     4021 ggcgtcgcgt tggtcggcac gctcgccgag ttcctcgccg acaagatcgc gtgggtggat
     4081 tcggcctggg acacggttca cagcatcgtc cggcccctgg gcggcgcgct cctcgcgctt
     4141 gccctcgtcg attcctccga tccggcgtgg caggtcatcg ctttcctgct cggcggcggc
     4201 ggcgcgctgc tcagccacgg agcgaaggcg acgacgcgcg cggtcgtcaa cgtcagcccc
     4261 gagcccttca gcaacgccgc ggtctcgacc ggcgaagatg tcgcgacagg cgggctgctg
     4321 gcgctcgcca tcgcctatcc gccgctggcg atcgtcatcg cggtcctgct cgcggtcgcc
     4381 gcggtcgtgg taatcatcgc gctgcgccgc ctgctccgca atatcaaggc gacgctgaag
     4441 cgcgcgctgg gcgacgagcc tcccccggca atcgatgctt aacagtttgg caatattcgg
     4501 ttaataagtc tcgacgagca ggggggcaca ccgacccgat agggagtgat gtgctatgtc
     4561 cccgaaatat tccctcaggc cgatactcct gtcttccgtc gccgccctcg cgctgggaca
     4621 ggcctctccc actcttgcgc aatgcgtctt cacgcccacc gccgatgacg atgtccatat
     4681 ctgcgacacc ggcacgtcgg tgggcggcct cgacgatccg gacggaaaca accagctcac
     4741 cctccccgtc ggcggcaccg gcaccgtcgc gggcgacgtc agcttcggcg gcggcgccga
     4801 catcgtcgtc atcgattccg gccggatcga aggcgcagtc gatcaaggca atggcaacga
     4861 cagcttctcg atcagcgccg gaaccgtcac cggccaggtg cagcaaggcg aaggcatcga
     4921 cgacttcgcc atgaccggcg gcgagatcga ccggctcaac caaggcggtt cgatggatac
     4981 gttcctgatg accggcgggc gcatcgtcga tgccttcgac gacggcgaca cggcgcggat
     5041 gaccggcggg cgcatcggcc gcgtcaatat gaagcttgcc gacaatctct tcgacatgtc
     5101 gggcgggacc atcgaccgca acctcgtcgc cggcttcggc aacgacacga tcatcctgtc
     5161 cgccggaacg atcggcggca atatcagcgt gtcgggcggt accgacagcg tgacgatcac
     5221 cggcgggtcg gtgggcggcg acgtgctgat gagcgtcggg accgacagct tcgtctggga
     5281 cggcggcggc gtcgtccacg gcacggtcga tctgggcggc gacgacgaca gcgcccggct
     5341 tgccaacctg accaatgcga acctcggcgg gtcgccgcgg atcaccggcg gggcgggcag
     5401 cgatgcgctg gttttcgaca atgtcacgac ggacggggtc gcgcgcttcg acgggtggga
     5461 gaatatccag gcgacgaacg acagcgaact gacgttcgac ggcttgctga cgctcggcga
     5521 cgccgggacg ggcaccgggc gcttcgaact cgacgcgtcg agcacgatct tcggcggcgg
     5581 ccagcggggc gggatcgtcg ccttcacggc gggcgaacgc gcgacgctga tcaatgccgg
     5641 acgcatcgat ctgaccaatg gcggcaatgt gccgggagac agctttaccg tcggcggcga
     5701 ctatgtcggc aacgatggac tgttgctgat cgataccgtg ctgggcgacg acgtatcgct
     5761 ttccgacaag ctggtgattg acggcggcag cgcttcgggc accaccggga tcaccgtgct
     5821 caacgcgggt ggaaccggcg ccctcaccca gcagaacggc attctcgtcg tcgaggcggt
     5881 gggtggcgcg acgaccgcga gcggtgcttt cgcactcaac aaccgcctcg ccgtcggcgc
     5941 ctatgaatat tttctgttcc gcggcggcac cacgaccggc accgacgaga attggtatct
     6001 ccgctcgaca ttgccgccac cgcctgagcc gacgcccgag cccgaaaccc cgccacc
//
LOCUS       DATMSU010000268         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_70949, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000268 DATMSU010000000
VERSION     DATMSU010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70949"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..581)
                     /locus_tag="VN034_07200"
     CDS             complement(282..581)
                     /locus_tag="VN034_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60420.1"
                     /translation="MSEIAARLGQDRCEEREEGIALGGAFGADQAGAGEDRAVLAILR
                     PGTLQRPQREALAHFRGIEGRGGKQRARQRIDLALAERDGGSVRRGGHDGSYVLS"
     gene            594..1253
                     /locus_tag="VN034_07205"
     CDS             594..1253
                     /locus_tag="VN034_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60421.1"
                     /translation="MRQIAIRPLAAEANETRAIVDAGAEELAGVRAVAESPKLPIDPV
                     SRVLTIVRSLRRSADVDVDPLAATAFSPCWAVNLFAVADPVLLGLSPHPLPFPGLVRR
                     RLFRADRDPEADREVAGELLLEAFHETLCDIARIPRAATAFGETFENLRGNSRLSSAW
                     MLLFAFGSMTPAQLARALPCTKAGAGKLLRQLRDGQFAVNNGPQEPFGCSRQFPVSFS
                     I"
     gene            1421..2104
                     /locus_tag="VN034_07210"
     CDS             1421..2104
                     /locus_tag="VN034_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV60422.1"
                     /translation="MPIISIANPKGGAGKTTLAFILADQLSSNGASVAVVDADPNAII
                     AKWHERRTAEGRASPFTVIAKPSEGAMINTVDQLAKEYQFVIVDLEGTASRMTSRAFA
                     RSHLVLVPFNQSPIDAELAANAVQLIHEESEALQRPLPFRLVRSRDSSAVTTKSSKRI
                     ATAIAEANLPVLAVGLVERAAYRDIFDFSKTLGELESDTTSGLENARGNAAKLAASVI
                     EAIREEYPQ"
     gene            2101..2430
                     /locus_tag="VN034_07215"
     CDS             2101..2430
                     /locus_tag="VN034_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60423.1"
                     /translation="MSGYGFGSKKPAGDKAAGTMDLGGLPGSLPPLKREDEARAVAVG
                     ETLGFVDRGQGVRRRKPPAMPSVNLYVKGPQDIIQWFIDYTDAQGHSAYWKSIEDFKR
                     HMEGEGR"
     gene            complement(2466..3557)
                     /locus_tag="VN034_07220"
     CDS             complement(2466..3557)
                     /locus_tag="VN034_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HWV60424.1"
                     /translation="MEVSSGFESLSRTLRVAEVIRRRELDFVSKPGELVESEFETGSL
                     SAAARKAFAIMLRKAGPEAGDDKTFVITKRELRGSHKGNERIQPVMDELLRVIIRIRT
                     TSTKGKPAIMSQSLLASCVEEIAEDGLSVVEFQFSDNFKRVIQRSDYYARVNLGVMLA
                     LQSRYAITLYDLGCLVINRQNRVVKMAVEELRRKLGVPDGSFKNFAEFRRDVLAKSKS
                     EIDQLADFTVDWEEVRGAGRGRPVIAVKLSFNPKDTVDQEMTAQELDRPKVGRRARRE
                     GSVDAIVGVLPPSLQGRGPFPEGSLHYGADPRITSIVVDFGGGWDKDVIADAYRKEMG
                     GRLEKLTGEALYKSWEGFCKSFVRNRGRA"
BASE COUNT          710 a         1106 c         1016 g          740 t
ORIGIN      
        1 ccagctcgcg cgcgatcgcg gcggccgagc gccgcgtgcc ggcataggcg ccgaagagct
       61 ggcgcaggtc ggccgcgatg cggtgatcga tcttcatggc gcgccctcct tttcccggct
      121 tccttggggc ggaattttcg accgaaaggg ccgatttggc aagctcggtt ttaccataat
      181 gtccgattat ggaaagagcg ggattcacgg tgcggaatga tgggaaacca aactggcagt
      241 ttggtgaact cgaactttga cgccgactcc gctcccggct atcaagatag cacataggag
      301 ccgtcatgcc cccctcgcct cacactgccg ccgtcgcgtt ccgccaaggc cagatcgatg
      361 cgctggcgcg cacgctgttt gcctcccctg ccctctatac cccggaaatg cgccaatgct
      421 tcgcgctgcg gtcgctgcag cgtgcctggt cgcaatatcg cgaggaccgc gcgatcctcg
      481 ccggcgccag cctgatcggc gccgaatgcg ccgccgagcg cgatgccttc ctcgcgctcc
      541 tcgcatcgat cctgccccag cctcgcggcg atctcggaca agcgatcgcg cgcgtgcgcc
      601 agatcgcgat ccgccccctc gcggccgagg cgaacgagac ccgtgcgatc gtcgacgccg
      661 gcgccgaaga gctcgcgggc gtccgtgcgg tcgcggaaag ccccaagctt cccatcgatc
      721 cggtctcgcg ggtgctcacg atcgttcgca gtcttcgccg atcggccgac gtcgacgttg
      781 acccgctcgc ggcgacggca ttttctccat gctgggcggt caacctgttc gccgtcgccg
      841 atccggtgct gctcggcctt tcgccgcatc ccctgccctt ccccgggctt gtccgacgtc
      901 ggttgttccg cgccgaccgc gatcccgagg cggatcgcga ggttgcgggg gaattgctgc
      961 tcgaggcgtt ccacgagacg ctgtgcgaca tcgcgcggat tccgcgcgcg gcgaccgcct
     1021 ttggcgagac ttttgagaat ttgcggggga attcacgcct gtcttccgcc tggatgctgt
     1081 tgtttgcgtt cgggtcaatg acgccagccc aactggcaag agcccttccc tgcactaagg
     1141 caggcgcggg gaagttgctt cgacaattga gagatgggca gtttgccgtg aataacggtc
     1201 ctcaggagcc gtttggatgc tcgaggcagt tccccgtgtc gttctcgata tagcgccacg
     1261 tcaaactcgg gcccttcgat agaaagccaa tcgattgcga ttggattgct atatggatat
     1321 cctttccatt gttatagcat atcgctcgcc aatggcttgc gcttgcgcaa gaaaaggcat
     1381 atgcaaccca tatctcaatc gaaagctata tggatatgct atgccaataa tctccattgc
     1441 caatcccaag ggcggcgccg gcaagacaac tctcgcattc atcctcgccg atcagttgag
     1501 cagcaacggt gcatcggttg ccgtcgtcga tgccgatccg aacgcgatca ttgccaagtg
     1561 gcatgagcgc cgcaccgcgg agggtagggc gtctccgttt accgtcattg caaaaccgtc
     1621 agagggagcg atgatcaaca ccgtcgacca gctggcgaaa gagtatcaat tcgtgatcgt
     1681 cgatcttgag ggcaccgcgt cacgcatgac atcgcgggcg tttgcacggt cccaccttgt
     1741 gctcgtccca ttcaaccaat caccaatcga tgccgagttg gcggccaatg cagttcaact
     1801 cattcatgag gagtcagagg cactgcagcg gccgctacct ttccgcctgg tgcggtcgcg
     1861 cgatagcagc gcggtcacga ctaagagctc gaagcggatt gctacagcga tcgccgaagc
     1921 caatctgccg gttctcgccg tcggtctggt cgagcgggca gcgtatagag acatcttcga
     1981 tttttcgaaa actctgggcg agctcgaatc ggacaccact tctggccttg agaatgcgcg
     2041 tggcaacgcc gccaaattag ctgcctccgt gatcgaggcc attcgggagg agtatccgca
     2101 atgagcggct atggatttgg ctcaaagaag ccagcgggtg ataaagcagc aggcacgatg
     2161 gatcttggag gtctccccgg ctccctaccg ccgctgaagc gtgaagatga ggcgagggcg
     2221 gtcgccgtgg gagagacttt ggggtttgtc gatcgagggc agggggttcg ccgccggaaa
     2281 ccgccagcga tgccatcggt gaacctctat gtgaaaggcc ctcaggacat catccagtgg
     2341 ttcatcgact acaccgacgc ccaaggtcat tcagcctact ggaaatcaat cgaagatttc
     2401 aaacgccaca tggaaggcga gggccgatag ccctttatgt gagaaactgg gggcgagctt
     2461 aagcgtcagg cacgcccgcg atttcgcacg aaggatttgc agaagccttc ccaggatttg
     2521 tagagggcct ctcctgtcag tttctcgagg cggccgccca tttccttccg gtacgcatcg
     2581 gcaatcacat ccttgtccca gccaccgcca aaatcgacga cgatggacgt aatccgcggg
     2641 tcagcaccat agtgaagtga cccctccggg aacggacctc gaccttgaag cgacggcggc
     2701 aagacaccta caatcgcgtc aactgagcct tcacgacgtg cccgtcggcc aactttcggg
     2761 cgatcgagtt cctgcgccgt catctcctgg tctaccgtgt ccttcggatt aaagctaagc
     2821 ttcaccgcaa tgacggggcg accccgtccg gctccacgaa cctcctccca atccaccgta
     2881 aaatcagcga gctgatcgat ttccgatttc gatttggcga ggacgtccct ccggaactcc
     2941 gcaaaattct tgaagctacc gtcgggtact ccaagtttgc gtctgagttc ttcgacggcc
     3001 atcttcacga cgcgattttg tcgattgatg acgagacagc ccaaatcata gagcgtgatc
     3061 gcatagcgag actgcagagc gagcatcact cccagattga cccgagcgta gtagtccgat
     3121 cgttgaatca cgcgcttgaa attgtccgaa aactgaaatt ccacaacgct caggccgtct
     3181 tccgcgattt cttcaacgca gctcgctaga agcgactgcg acataatcgc tggcttgccc
     3241 ttcgtcgaag tagttcggat gcggatgatt actcggagga gttcgtccat caccggttga
     3301 attctctcat tgcccttatg ggatccgcgc agttccctct tcgtaatgac aaaagtctta
     3361 tcgtcgccag cctccggccc ggccttcctg agcatgatcg caaatgcctt acgggcagcg
     3421 gcgctaagcg aaccggtctc aaattcggat tcgaccaact cgccgggctt gcttacaaaa
     3481 tccagctcac ggcgccgaat cacttccgca acgcgtaacg tccggctgag gctctcaaag
     3541 cctgacgaaa cctcgatttc cgatgtcgga gt
//
LOCUS       DATMSU010000269        18986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_42863, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000269 DATMSU010000000
VERSION     DATMSU010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 18986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18986
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42863"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..348)
                     /locus_tag="VN034_07225"
     CDS             complement(85..348)
                     /locus_tag="VN034_07225"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWV60425.1"
                     /translation="MTDTPDSAASPDIASLSFEAAMGELETIVRRLESGDVSLEESVT
                     LYERGHALRGHCEARLAAAQARIEQVSLSADGRPTGTTPFGES"
     gene            813..1109
                     /locus_tag="VN034_07230"
     CDS             813..1109
                     /locus_tag="VN034_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60426.1"
                     /translation="MKKFILPALGLIALAPTAALARDEPVKRFEHEGATYSYTVTKVG
                     DTRVISGVEERTGKPFSLRVGSHRVRGTVGSQQVSFTLRDVEPLKIETTTLAAR"
     gene            1193..3436
                     /gene="purL"
                     /locus_tag="VN034_07235"
     CDS             1193..3436
                     /gene="purL"
                     /locus_tag="VN034_07235"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWV60427.1"
                     /translation="MTQQAPAITPEIVAEHGLSPAEYDRVLNAIGREPNLVELGIFSV
                     MWSEHCSYKSSRIHLKKLPTEAPWVICGPGENAGVIDIGEGQGGKKLAAIFKMESHNH
                     PSYIEPYQGAATGVGGILRDVFTMGARPVANLNALRFGRPDHPKMKHLISGVVHGIGG
                     YGNCVGVPTVGGEVNFHKAYDGNILVNAMTVGVAEQDKIFYSAASGVGNPIVYVGSKT
                     GRDGIHGATMASADFGEDAEEKRPTVQVGDPFTEKLLIEACLELMASDAIVAIQDMGA
                     AGLTSSSVEMASKGGVGLHLKMDDVPQRETGMTAYEMMLSESQERMLMVLKPGREDFA
                     AAIFHKWELDFAVIGTVTDTGRMVLEHHGEIVCDIPLAPLADDAPLYDRPHVPTPKQA
                     ELTDVPETKDVAADLKILMGTPDIASRRWIWEQYDSQVGADTVQTGGDAALVRIHGTN
                     RALAMSTDCTPRYCYADPVEGGKQAVAETWRNISAVGATPLAITNCLNFANPQRPEIM
                     GQITGCLDGMAQACIALDYPIVSGNVSLYNESKATGGGSAILPTPAIGGVGVIEDLNK
                     AVGIGFKRTGDIVLAVGERAGHLGQSVWLREILGREEGPPPPVDLRAEKRTGDFIRGA
                     INAGWITACHDVSDGGMAVTLAEMALKSNIGVLVSEEQPFGVAESFFGEDQGLYLVTV
                     CDTCLADFLDAAGRADVPVDPVGRTIKDRIVFELEGSDHQVTLAELREAHEGFFPNLM
                     GADAALA"
     gene            3534..4271
                     /locus_tag="VN034_07240"
     CDS             3534..4271
                     /locus_tag="VN034_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWV60428.1"
                     /translation="MSIHIGVGGWTFEPWRGPFYPAGLPQKRELEYAGQHLTGIEING
                     TYYGSQKPETFANWAEAVPDGFKFSVKASRFTTNRKVLAEGAASIEKFLTQGLTRLGD
                     RLGPIHWQFMATKKFDRDDFAGFLDLLPDSQDGLKLRHAIEVRDESFRDPAFIDLLRE
                     RNMAVVYADSDEFPCIDEQTADFTYARLQRSQEDVETGYDDKALDGWAKQARDWAKGG
                     RDVYLFFISGAKVRNPAAAQALIAKLG"
     gene            complement(4196..4615)
                     /locus_tag="VN034_07245"
     CDS             complement(4196..4615)
                     /locus_tag="VN034_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV60429.1"
                     /translation="MTTRNQPYNDGLGPVFEIIGGKWKALILWELHDTPVRFGALKRR
                     IAGISEKMLIQQLREMEADGLVHREMFHQVPPRVDYSVTPLGASLNEALTPLCEWGKH
                     NLQPRLPAGGDAGQPSLAISACAAAGLRTFAPDMKKR"
     gene            4750..5397
                     /locus_tag="VN034_07250"
     CDS             4750..5397
                     /locus_tag="VN034_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HWV60430.1"
                     /translation="MPEKLLILVGSPRRNGNSATLADAVRRGAEAVGSDVTLRFLDDH
                     ISGFLRDCRQCRRPDGECAIDDPYRALFFEDFLPARGVIFCSPIYWYGVSAQIKAFFD
                     RSFCYYAASYPGSQDALAAMAGKRLGLVLASEETYPGVALGIVHQIQEFSRYTNSDFV
                     GVVRGAGNSRGEVRRDPSDPVGDAERLGREFFARSYSDYRLETPRSPRVWPEQAA"
     gene            complement(5453..6232)
                     /locus_tag="VN034_07255"
     CDS             complement(5453..6232)
                     /locus_tag="VN034_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HWV60431.1"
                     /translation="MIPTIFSHPDRQPQKFRPFKAFQHFRKLIKDKEDTEQVFHIFEN
                     LPRKGFMDDARAFVESDFGRKLMEREPYLPDLLDDHGWIDELPEGTVGHAYVTFMRRE
                     GLSAGGLVAESDKMGRPQFDDQVQWYSNRLRDTHDLFHILTGYGRDALGEQCVLGFTY
                     GQTGNYGNFFIAYAGGYEVKRGVKSDAPVFGAIRQGQRHGKAAKAIIEQDIRALLAEP
                     LEAARARLGIGEPTLYQEAHRAYRNRGIDPYNFLAAQAAAA"
     gene            6388..7644
                     /locus_tag="VN034_07260"
     CDS             6388..7644
                     /locus_tag="VN034_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HWV60432.1"
                     /translation="MEGQAAGTRYESLDAIRGVAVMGILAMNIVAFALPFPAYANPAA
                     GGPPGSADAATWFFNFVFVDSKMRGLFSMLFGASTLLVIDSAASAGRSAAGAHYSRMF
                     WLAIFGLIHFYLIWFGDILFLYAMCGLLLFLFRDLSVKALLLWSIPFLLIGIALPGSF
                     WASLAMAQAGTLPPEAAGAMRGALTQMNADMGPSSPAYAQEMALHLGSYASIVAHRTG
                     EMAGDPLFFVSLFAWETIGLMLIGMALFKSRMLTGEWEAARYRKWALISFLVAAPPLA
                     GLALYQMNMGYDAVAIFGSTVALSVPFDTLMTVGWAALIMLAIQARASAAVRARLAAT
                     GRMAFTNYLVTSIVMTTIFYGYGLGLFGSVGRLPLYLFCFGMWAAMLLWSKPWLERYH
                     YGPLEWLWRSLSRWRVQPMRKAAITQ"
     gene            complement(7638..8039)
                     /locus_tag="VN034_07265"
     CDS             complement(7638..8039)
                     /locus_tag="VN034_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWV60433.1"
                     /translation="MDEILVDWDEFRATRAQLGAAFVRILGYFREDGTKSVAAIEEAM
                     RARDARGLVMPAHTLKSEARQFGGEKLGALAEDIEMFARACVESQTSPDEYLPRVVEL
                     RRLFEATLGALEREANPLVQRRPSFGRAVNY"
     gene            complement(8423..8650)
                     /locus_tag="VN034_07270"
     CDS             complement(8423..8650)
                     /locus_tag="VN034_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWV60434.1"
                     /translation="MTSDGAALTVDARGMRCPWPALRLARAMRDACEVVLIADDPQAG
                     REVAALADEHGWLVEGDVASADTGRWRVRRG"
     gene            complement(8640..10025)
                     /gene="der"
                     /locus_tag="VN034_07275"
     CDS             complement(8640..10025)
                     /gene="der"
                     /locus_tag="VN034_07275"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWV60435.1"
                     /translation="MSRHATIAIVGRPNVGKSTLFNRLVGKRLALVDDQPGVTRDRRE
                     GDAKLLGLEFRIVDTAGFEDQDAATLPGRMRVQTEKAVREADAALFMIDGRAGVTPLD
                     EEIARWLRSEDTPIILLVNKAEGKQGENGLMESYSLGFDNPIALSAEHGEGVVDLFDA
                     LRPIVESHDAEEAGALPLVDGEEDEDAPLGPMKLAIVGRPNAGKSTLINRMIGEDRLI
                     TGPEAGITRDSIRVDWQWEKDGEVHEIQLFDTAGMRKRAKVVDKLEKLSVADALHAVD
                     FAEVVVLLLDATKGLEAQDLRIADKVLQEGRALIVALNKWDIAEDPSSLFNGVRTALD
                     DGLSQVKGVPVLSISGATGKGIDTLVRVAFEQREIWTNRVSTARLNRWFEGAVTANPP
                     PAPGGKRIKLRYITQARTRPPTFVVFGSRTDSLPGSYERYLVNGMRKELGFQGVPVRL
                     NFRNSRNPYDE"
     gene            complement(10217..11551)
                     /locus_tag="VN034_07280"
     CDS             complement(10217..11551)
                     /locus_tag="VN034_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWV60436.1"
                     /translation="MRSARYGLYATLLALPLAACGVFKGDGPPKTPTVGERVSILSND
                     NSIKVDPATAAMAVVLPEPAVNAAWAQSGGNASKSMGHPALGATRAKLWEASIAGSTN
                     KQRLASSPVVADNRLFVVDTDAVVSAFAADTGAKLWSAAIGSTGKDFKDSLFGGGAAV
                     DGNVVYATSGVGDVAALNAADGSVIWKVKPTGPLRGAPTIAFGGVYVISQDNQIYALS
                     AANGAVQWQATASTEAGSVFGAASPAAGQGTIVAGFSSGEVQAYRYENGRDLWEDALA
                     RTSMALSVSTLTDVDADPVIDRGHVFALGQGGRMASYELVTGQRSWEISIAGISTPYV
                     VGEWVYAMTDDAKLLCVARSSGKVRWLQQLARFRIETEKKKKDPIRWTGPILAGGRLI
                     AVNSEGTLAEYSPTDGSLLASTEFDSSLSQSPVVANNILYVLADDGTITAWR"
     gene            complement(11568..12341)
                     /locus_tag="VN034_07285"
     CDS             complement(11568..12341)
                     /locus_tag="VN034_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV60437.1"
                     /translation="MALSPTNDAALLQEVDEAVRKDRLDTIMQRYGRWIIGGVLAALL
                     AFGGYLFWDHRQDAARGEQAEELIAAFEKLATNQPRAATAELQKIAAEGGPAYRAVAQ
                     MQEANIKAQTGDLKGAAALMAKVAADTKLDKALRDLALIRQTAFEYDTLKPEVVIARM
                     KPMVDAKDPASSWFASAAELSATAHYQLGQFDQAGALYGRIAKSPDVTRSLQSRSVQM
                     AGMLGVDAVTDRAAESAAAEEKGNAAPKAAAASKTEKAN"
     gene            complement(12455..13318)
                     /gene="panB"
                     /locus_tag="VN034_07290"
     CDS             complement(12455..13318)
                     /gene="panB"
                     /locus_tag="VN034_07290"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWV60438.1"
                     /translation="MSTLPKTLTLDTSTSRANPTPQPMKRLTVPRIRQRKGGEPLVML
                     TAYTVRMAQLLDPHCDMLLVGDSLAQVIYGLPHTVGVTMDMMALHGAAVVRGSYHAAV
                     IVDMPFGSYEGSPEQAFDNAARLLKETGAAAVKVEGGKVLAPTIEFLTQRGIPVMGHV
                     GLTPQAVNILGGYGVRGKSAEEARSIVEDAVAVAQAGAFSIVIEGVLESIAIEITNKV
                     DCPTIGIGASAECDGQVLVTDDMLGMFERVPKFVKRFGNMAGVVESAVKDYADEVRSR
                     SFPTEDQTYAG"
     gene            complement(13396..14325)
                     /locus_tag="VN034_07295"
     CDS             complement(13396..14325)
                     /locus_tag="VN034_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWV60439.1"
                     /translation="MTEHALDQSGLSIRPFVLGGNVFGWTADRTASFAIFDRFVEQGG
                     GMIDTADVYSAWVPGHKGGESEAMIGEWLKASGARDQVLIATKVGMGEGGLRPDRIHA
                     AVQGSLDRLGVDVIDLYFAHKDDTDVPLDETLGAFGELVDAGKVRAIGASNYSAERLA
                     EALRVSAEKRLPRFTVMQPELNLLDRDQYEGPLQELCIAEEIGVIPYYSLAAGYLTGK
                     YRGAEDLGQSARGYKAKAYMAGRGPAVLAVMDAIAAETGATLSQIALAWVVAQPGVTA
                     PIASATTVGQLDELMGSLDLDLNDQQLAALSGA"
     gene            complement(14322..15398)
                     /locus_tag="VN034_07300"
     CDS             complement(14322..15398)
                     /locus_tag="VN034_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007711201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWV60440.1"
                     /translation="MIRSIAAVAALMLSTGCTADDVTAQPTLDGRPPIVIAHRGASGE
                     RPEHTLASYRLAIEQGADFIEPDLVLTKDGILVAQHENEISETTDVADHPEFAGRKIE
                     KTIDGQKVEGWFTEDFTLAELKTLRARERLPRLRSTDYDGQFEIPTFEEILTLLAEVN
                     KDRDRPVGVYPETKHPGYFVAIGLPHEAPLLAMLDRFGYRGRTAPVFIQSFEVGNLID
                     LRAKSDLPLIQLMDAGGGPADRPGTSYAAMASPAGLKMIAGYADGIGPNKAMVIPRGT
                     LGRLGKPTALVRDAHAAGLKVHPWTFRRENYFLPLGDKASINPAGHGDLAGEIDAYLT
                     AGVDGLFSDNPREAVAAVAKGASK"
     gene            complement(15395..15745)
                     /locus_tag="VN034_07305"
     CDS             complement(15395..15745)
                     /locus_tag="VN034_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HWV60441.1"
                     /translation="MTMILYGISNCDTLKKARRWLDERGVAYRFHDVRKDGLDAARLQ
                     GWIDALGWEKLLNKAGTTFRKLPEEQKEGLDAASAKALMLEQPAMIRRPVVENGGDIS
                     VGFSAADWQARFAA"
     gene            complement(15813..16478)
                     /locus_tag="VN034_07310"
     CDS             complement(15813..16478)
                     /locus_tag="VN034_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWV60442.1"
                     /translation="MTNFDSAWFLYGALLLLLATSFVVRIDYARIGLAAAALLALPLT
                     LFRGADLGYSLLVLAILAVNIGILARLWLRGTKVRFSAEEEGLRREYFNGLGTVAARD
                     LIDQGHWISAKRGEVLIRENQAAPSLFYLAEGQAAVLRDGVEVGKLSGGALIGEATAL
                     DGSHATGTVVLGSNARLWFVPAAALRAYLTANPAIAGALHEGFARALRGKLASANTRI
                     AER"
     gene            16749..18122
                     /locus_tag="VN034_07315"
     CDS             16749..18122
                     /locus_tag="VN034_07315"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HWV60443.1"
                     /translation="MTKNWQPHSWRSHEARQLPTYRDADALAAAERELANYPPLVFAG
                     EARELTSELGRVAEGKAFLLQGGDCAESFAEFHPNNIRDTFRVLLQMAVVLTFASKMP
                     VVKLGRMAGQFAKPRSADLEDIDGVELPSYRGDIINDIAFEEAGREPDPQRMVKAYNQ
                     SAATLNLLRAFANGGYANLHQVNAWTHDFMDRSPWAKKYQETAARISEALAFMEACGV
                     TPETVPQIKGTSFYTSHEALLLPYEQALTRQDSLTGGWYDTSGHFLWVGDRTRFEGSA
                     HIEYLRGIGNPVGMKCGPSLEPDALLRLLDTLNPNHVAGRMTLITRYGHDKIEAHLPK
                     LVRAVKESGHPVVWSCDPMHGNVIKTSTGYKTRPFERILAEVRGFFAVHRAEGTHGGG
                     IHIEMTGQNVTECTGGAMDVTQMDLADRYHTHCDPRLNAGQSLELAFLLAEMLNQEMA
                     DRAKQAA"
     gene            complement(18160..>18986)
                     /locus_tag="VN034_07320"
     CDS             complement(18160..>18986)
                     /locus_tag="VN034_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWV60444.1"
                     /translation="EAAGTLLKAAGQDGAALREAAKSPDYKGAALPVTADFTAGSAVR
                     AFASHNIIAKLPGAKPDGKAVLFLGHWDHLGICEPEGAADRICNGAVDNASGIAVLIE
                     VAKRLGNGTRPDRDIYFMATTAEEKGLLGAHYFADHSVVPLSDITVALNVDTIAISPR
                     GTPVATIGRGKPAYDAVVREVATKLGRKLDEDGEADAFIQRQDGWALGAKGVTSLMVG
                     GSFSDMKLLEAFLGSDYHQAADNFSDKIPLGGAAEDADLHVALGRAFADTKRWPGK"
BASE COUNT         3197 a         6437 c         6003 g         3349 t
ORIGIN      
        1 gatcccggcc acgacctcgg cctgcgtcga cagcagcaat tgcgtgccgc gcgaaagctg
       61 atcgtcggcg agcgccatcg ccgatcagct ttcgccgaag ggggtggttc ccgtgggccg
      121 gccgtcggcg ctcaggctga cctgctcgat ccgcgcctgc gccgcggcaa gccgcgcttc
      181 gcaatggccg cgcagcgcat ggccgcgttc atagagcgtc accgattcct cgaggctgac
      241 gtcgccgctt tcgagccgcc gcacgatcgt ttcgagctcg cccatcgccg cttcgaaaga
      301 gagcgaggcg atgtcaggcg aggctgcgct gtcgggggta tccgtcatgc cccctgcttt
      361 ggccccttcg cccccttgcg gtcaagtgcg gtcgagtttg acgccgcttt cggccgccat
      421 tgtcaccgtc ccagctgcgg ccgctttggg gtgggattga ggacgtttag gagttgagct
      481 ggtttcccct cccgcaggcg ggaggggcag cgaaacttgc gaacttgttc gctagttgca
      541 gcggggtggg caatcgcaac gtcgctgccc acccccggcc cctcccgctt gcgggagggg
      601 agacaatgtc agcttccggc cgaaagcgga cgtccgtcgc gacaagaccg ctacctccaa
      661 cgtggggagt ccctacccgg cccccattta ccccgcgcaa tcacgcttcc gttttttgca
      721 attgccgctc cccacatccc cagccatgtt gcaccgcaca agaagagagg ggctcgaagg
      781 caagttttgt tccaccgaca aggacttgca ccatgaaaaa attcattctc cccgccctcg
      841 gcctgatcgc gctggcgccc accgccgcgc tcgcccggga cgagccggtc aagcgtttcg
      901 aacatgaagg cgcgacctac agctatacgg tcaccaaggt gggcgacacc cgcgtgatca
      961 gcggcgtcga ggagcgcacc ggcaagccgt tctcgctccg cgtcggcagc caccgcgtgc
     1021 gcggcaccgt cggctcgcag caggtcagct tcacgctgcg cgacgtcgaa ccgctcaaga
     1081 tcgagacgac caccctcgcc gcgcgctgaa ccctctctcc gaccgcagac cccatgatgt
     1141 tttcgcatca tggggtctgt tcgttttgcg cttgggtcgc taaaggcgcg ccatgactca
     1201 gcaagcgccc gccatcaccc ccgaaatcgt cgccgagcat gggctttcgc ccgcggaata
     1261 tgatcgcgtc ctcaacgcga tcgggcgcga gccgaacctc gtcgaactcg gcatcttctc
     1321 ggtcatgtgg tccgagcatt gcagctataa aagctcgcgc atccacctca agaaactgcc
     1381 gaccgaggcg ccgtgggtga tctgcggccc cggcgaaaat gccggtgtca tcgacatcgg
     1441 cgaaggccaa ggcggcaaga agctcgccgc gatcttcaag atggagagcc acaaccatcc
     1501 gtcgtatatc gaaccctatc agggcgcggc gaccggggtc ggcggcatcc tccgcgacgt
     1561 cttcaccatg ggcgcgcgcc ccgtcgcaaa tctcaacgcg ttgcgcttcg gccgccccga
     1621 ccatccgaag atgaagcatc tgatctcggg cgtcgtccac ggcatcggtg gttatggcaa
     1681 ttgcgtcggc gtccccaccg tcggcggcga ggtcaatttc cacaaggcct atgacggcaa
     1741 tatcctcgtc aacgcgatga ccgtcggcgt cgccgagcag gacaagatct tctattcggc
     1801 cgcgagcggc gtcggcaatc cgatcgtcta tgtcggctcg aaaaccggcc gcgacggcat
     1861 ccatggcgcg acgatggcgt cggccgactt cggcgaggat gccgaagaaa agcgcccgac
     1921 ggtgcaggtc ggcgacccct tcaccgaaaa gctgctgatc gaggcgtgcc tcgaactgat
     1981 ggcgtcggac gcgatcgtcg cgattcagga catgggcgcc gcgggcctca cctcgtcgtc
     2041 ggtcgagatg gcgtcgaagg gcggcgtcgg cctccacctc aagatggacg atgtgccgca
     2101 gcgtgaaacc ggcatgacgg cgtatgagat gatgctgtcc gaatcgcagg aacgcatgct
     2161 gatggtgctg aagcccggcc gcgaggattt cgccgccgcg atcttccaca aatgggaact
     2221 cgacttcgcg gtcatcggca ccgtcaccga tacgggccgc atggtgctcg agcatcatgg
     2281 cgagatcgtc tgcgacatcc cgctcgcccc gctcgctgac gatgcgccgc tctacgaccg
     2341 tccgcacgtc ccgacgccga agcaggccga gctgacggac gtccccgaga cgaaggacgt
     2401 cgcggccgac ctcaagatct tgatgggcac ccccgacatc gccagccgcc gctggatctg
     2461 ggagcaatat gacagccagg tcggcgccga cacggtgcaa acggggggcg acgccgcgct
     2521 cgttcgcatc catggcacca accgcgcgct cgcgatgtcg accgactgca ccccgcgcta
     2581 ttgctacgcc gaccccgtcg agggcggcaa gcaggcggtc gccgaaacct ggcgcaacat
     2641 cagtgcggtc ggcgccactc cgttggcgat caccaactgc ctgaacttcg ccaacccgca
     2701 gcgccccgaa atcatgggac agatcaccgg ctgtctcgac ggcatggcgc aggcttgtat
     2761 cgcgctcgac tatccgatcg tctcgggcaa cgtcagcctc tacaatgaat cgaaagcgac
     2821 cggcggcggc agcgcgatcc tgcccacccc cgcgatcggc ggcgtcggcg tgatcgagga
     2881 tctcaacaag gcggtcggca tcggcttcaa gcgcaccggc gatatcgtgc tcgcggtcgg
     2941 cgaacgcgcc ggccaccttg gccagtcggt gtggctgcgc gagattttgg gccgcgagga
     3001 aggcccgccg ccccccgtcg acctccgcgc cgaaaagcgc accggcgact tcatccgcgg
     3061 cgcgatcaac gccggctgga tcaccgcgtg ccacgacgtc tccgacggcg gcatggcggt
     3121 gacgctcgcc gaaatggcct tgaagtcgaa catcggcgtg ctcgtcagcg aagaacagcc
     3181 cttcggcgtc gccgaaagct tcttcggcga ggatcagggg ctttacctcg tgacggtgtg
     3241 cgacacctgc ctcgccgact tcctcgatgc cgcgggccgc gccgacgtgc cggtcgatcc
     3301 ggtcggccgc acgatcaagg atcgcatcgt cttcgaactg gaaggcagcg accatcaggt
     3361 gacactcgcc gaactgcgcg aggcgcatga gggctttttc ccgaatttga tgggagccga
     3421 cgcggcactc gcctgattcc caccgcgtca tcccggcgca ggccgggatc tcgctggtgc
     3481 ggcacatcgc gacgatgaga tcccggcctt cgccgggatg acggaggtaa atgatgagca
     3541 tccacatcgg cgtcggcggc tggaccttcg aaccgtggcg cggccccttc taccccgccg
     3601 gcctcccgca aaaacgcgag ctcgaatatg ccgggcagca cctgaccggc atcgagatca
     3661 acggcaccta ttatggcagc cagaaacccg aaaccttcgc caattgggcc gaggccgtac
     3721 ccgacggctt caagttcagc gtcaaggcgt cacgcttcac caccaaccgc aaggtgctgg
     3781 ccgagggcgc ggcgtcgatc gagaaatttc tgacgcaggg gctgacccgc ctcggcgacc
     3841 ggctgggccc gatccactgg cagttcatgg cgacgaagaa gttcgaccgc gacgatttcg
     3901 cgggcttcct cgacctgctt cccgacagtc aggacgggct gaagctgcgc cacgcgatcg
     3961 aggtccgcga cgaaagcttc cgcgatccgg ccttcatcga cctgctacgc gaacggaaca
     4021 tggcggtcgt ttatgccgac agcgacgagt tcccgtgcat cgacgaacag accgccgact
     4081 tcacctacgc gcggctccag cgcagccagg aggatgtcga aaccggctat gacgacaagg
     4141 cgctcgacgg ctgggcgaaa caagcccgcg actgggcgaa aggcggccgc gacgtctatc
     4201 tcttcttcat atcgggcgcg aaggttcgca acccggcggc agcacaggcg ctgatcgcca
     4261 agctcggctg accggcgtca ccgcccgccg gtagccgggg ttgcagatta tgcttccccc
     4321 actcgcatag cggcgtcagc gcttcgttga gcgatgcccc caagggggta accgaatagt
     4381 cgacgcgcgg cggaacctga tggaacatct cgcggtggac aagaccgtcg gcctccattt
     4441 cccgcaattg ctggatcagc atcttctcgc tgatcccggc gatgcggcgc ttgagtgccc
     4501 cgaagcgcac gggcgtatcg tgaagctccc aaagaatcag ggccttccac ttcccgccga
     4561 taatctcgaa caccggtccg agtccgtcat tatagggttg attgcgtgtc gtcatgccgc
     4621 caattcttac ttgaaggtta gtacctgaca gaatagtggg tagttgatca atcgaaagta
     4681 acacgccacc ccctttcgtc aacaagacgc ggcagccgcg ccgtgtccca cgcgaaagga
     4741 tcggccgata tgccggaaaa actgctcatt cttgtcggaa gcccgcgccg gaacggaaac
     4801 agtgcgacgc tggccgacgc cgtccgccgc ggcgccgaag cggtgggcag cgatgtcacg
     4861 ctgcgatttc tggacgacca tatttccggg tttctgcgcg attgccgcca atgccgccgc
     4921 cccgacggcg aatgcgcgat cgacgacccg tatcgcgcgc tgttcttcga ggatttcctg
     4981 cccgcgcgcg gggtgatctt ctgttcgccc atctattggt acggcgtgtc cgcccagatc
     5041 aaggcgttct tcgatcgcag cttctgctat tatgcggcat cctatccggg ctcgcaggac
     5101 gcgctcgcgg cgatggccgg taagcggctc ggcctggtgc tggcgtccga ggaaacatat
     5161 cccggcgtcg cgctcggcat cgtccaccag attcaggaat tttcccgcta caccaattcg
     5221 gactttgtcg gcgttgtgcg cggggcgggc aacagccgcg gcgaggtccg ccgcgacccg
     5281 tcggatccgg tcggcgacgc cgaacggcta ggcagggaat ttttcgcccg gtcctattcc
     5341 gactatcggc tggaaacgcc gcgcagcccg cgcgtctggc ccgaacaagc ggcctgatcg
     5401 ccagcggttt ccggcatcgg tccccggcct tcgcccggcc gatgtcggga attcaggccg
     5461 ccgcggcctg cgccgcgagg aagttgtagg ggtcgatccc gcggttgcgg tacgcgcggt
     5521 gcgcttcctg atagagcgtc ggctcgccga tcccgagccg cgcgcgcgcg gcttcgagcg
     5581 gttcggcgag cagcgcgcgg atgtcctgct cgatgatcgc cttcgccgcc ttgccgtggc
     5641 gctgaccctg acggatcgcg ccgaacaccg gggcgtcgct cttgaccccg cgcttcactt
     5701 cataaccgcc ggcgtaggcg atgaagaaat tgccgtaatt gcccgtctgg ccataggtga
     5761 agccgagcac gcactgttcg ccgagcgcgt cgcggccata gccggtgagg atatggaaaa
     5821 ggtcgtgcgt gtcacgcagc cggttcgaat accattgcac ctgatcgtca aattgcgggc
     5881 ggcccatctt gtcgctttcg gcaacaaggc cgccggccga caggccttcg cggcgcatga
     5941 aggtgacata ggcgtggccg accgttccct cgggcagttc gtcgatccag ccatgatcgt
     6001 cgagcagatc gggaagatag ggctcgcgtt ccatcagctt ccggccgaaa tcgctttcga
     6061 cgaaggcgcg cgcgtcgtcc atgaagccct tgcgcggcag gttttcaaag atgtggaaca
     6121 cctgttcggt gtcttccttg tccttgatca gcttgcggaa gtgttggaac gccttgaacg
     6181 ggcggaactt ctgcggctgg cggtcggggt gggagaagat ggtcgggatc atggcgggtc
     6241 tcgctcgaat catgtgatgc agcggagata gcattactga catagatgtc aataatgctt
     6301 taacgcgcac cggcccgccc aagaaaccgc tcgacgctcc tcgccccccg cgataccccc
     6361 cggcgctcgg ccgaaaggga aagcggcatg gaaggacagg cggcgggaac gcgttacgaa
     6421 agcctcgacg ccattcgcgg cgttgcggtg atgggcatat tggcgatgaa catcgtcgcc
     6481 ttcgccctgc cctttccggc ctatgcgaac cccgccgcgg gcggaccgcc gggcagcgcc
     6541 gacgccgcca cctggttctt caatttcgta ttcgtcgatt cgaagatgcg cgggcttttc
     6601 tcgatgctgt tcggggcgag cacgctgctc gtgatcgaca gcgccgcctc ggccgggcgc
     6661 agcgccgcgg gcgcgcatta ttcgcgcatg ttctggctcg cgattttcgg cctcatccac
     6721 ttctacctga tctggttcgg cgacatcctc ttcctctacg cgatgtgcgg gctgctgctc
     6781 ttcctgttcc gcgatctttc ggtgaaggcg ctgctcctct ggtcgattcc cttcttgctg
     6841 atcggcatcg ccttgccggg cagtttctgg gcgtcgctcg cgatggcgca ggcgggcacc
     6901 ctgccgcccg aggccgccgg cgcaatgcgt ggggccctga cccaaatgaa cgccgacatg
     6961 ggcccgtcgt cgccggccta tgcgcaggaa atggcgctcc atctcggcag ctatgcgagc
     7021 atcgtcgcgc accgcacggg ggagatggcc ggcgacccgc tattcttcgt cagcctgttc
     7081 gcgtgggaaa cgatcgggct gatgctgatc ggcatggcgc tgttcaaatc gcgcatgctg
     7141 actggtgagt gggaggccgc ccgctaccgc aaatgggcgc tgatcagttt cctcgtcgcc
     7201 gctccgccgc tggcgggcct tgccctttac cagatgaata tgggctatga cgcggtggcg
     7261 atctttggat cgacggtcgc gctatcggtc ccgttcgaca cgctgatgac cgtcggctgg
     7321 gcagcgctga tcatgctggc gatccaggcc cgggcgagcg ccgccgtgcg cgcgcggctg
     7381 gccgcgacgg ggcgcatggc cttcaccaac tatctcgtga cgtcgatcgt gatgacgacg
     7441 atcttctacg gctatgggct gggcctgttc ggcagcgtcg gccgtctgcc gctctacctc
     7501 ttctgcttcg gcatgtgggc cgcgatgctg ctgtggtcga agccgtggct cgaacgctat
     7561 cactacgggc cgctcgaatg gctgtggcgc agcctgtcgc gctggcgggt tcagccgatg
     7621 agaaaggccg cgatcactca gtaattcacg gcgcggccaa agctcggacg acgctggacg
     7681 agcggattgg cctcgcgctc gagggcgccg agcgtcgctt cgaacagccg gcgcagttcg
     7741 acgacgcgcg gcagatattc gtcggggctc gtctggcttt cgacgcaggc ccgcgcgaac
     7801 atctcgatat cctcggccag cgcgccgagc ttctcgccac caaactggcg cgcttcgctc
     7861 ttcagcgtgt gcgcgggcat gacgaggccg cgtgcgtcgc gcgcgcgcat cgcttcttcg
     7921 atcgcggcca ccgatttggt gccgtcctcg cgaaaatagc cgagaatccg cacgaaagcg
     7981 gcgcccaact gggcccgcgt cgcgcgaaac tcatcccaat cgaccaggat ttcgtccaat
     8041 gctttagtcc cttggtattc tagggtcagg acccgttgat tgcgcgttcg aggcgtcgaa
     8101 atggcaaaga tatcgccttc gggcggctgc cgcgggtagc atcgctaccc gcaaggtcgc
     8161 tcgggggcga gatcgaagcc atttcggcgt cccttcggga tttgatcgat tttgtccatg
     8221 gttgcgtcgg caaggcttgg aatattttca tatgcctacg ccttgcctcc taaccctgta
     8281 caaaatcgct tcaaacctcg aacgcgcaat caacgggtcc tgaccctcgt tcggccgtgg
     8341 tcccagccgg tccatgccca acttgggtaa aaaagacgtt acggtgacgg caatcgcacg
     8401 gccaaagccg ccccgttttg gatcaaccgc gccgcacgcg ccagcgcccc gtgtcggccg
     8461 atgctacgtc gccttcgacc agccagccat gttcatcggc caaagcggcg acttcgcgtc
     8521 ccgcctgcgg gtcgtcggcg atcagcacca cctcgcacgc atcgcgcatc gcgcgggcga
     8581 ggcgcagcgc gggccagggg cagcgcatcc cgcgtgcatc gaccgtcagc gccgccccgt
     8641 cactcgtcat agggattgcg cgaattgcgg aaattgagcc gcaccggcac gccctggaac
     8701 cccagttcct tgcgcatgcc gttgaccaga tagcgttcat agcttccggg taagctgtcg
     8761 gtccgcgaac cgaatacgac gaaggtcggc gggcgcgtcc gcgcctgcgt gatatagcgc
     8821 agcttgatgc gcttgccgcc cggcgccggg ggcgggttgg ccgtcacggc gccttcgaac
     8881 cagcggttga gccgcgcggt cgacacgcgg ttcgtccaga tttcgcgctg ctcgaacgcg
     8941 acgcgcacca gcgtgtcgat gcccttcccc gtcgcgcccg agatgctgag cacgggcacg
     9001 cccttgacct ggctgagccc gtcgtcgagc gcggtgcgga cgccgttgaa cagcgacgag
     9061 ggatcctcgg cgatatccca tttgttgagc gcgacgatca gcgcgcggcc ttcctgcagc
     9121 accttgtcgg cgatgcgcag atcctgcgcc tcaagccctt tcgtggcgtc gagcaacagc
     9181 acgaccactt cggcgaaatc gaccgcgtgc agggcgtcgg cgaccgacaa tttctccagc
     9241 ttgtcgacga ccttggcgcg cttgcgcatg ccggcggtgt cgaagagctg gatctcgtgg
     9301 acctcgccgt ccttttccca ctgccagtcg acgcggatcg aatcgcgggt gatccccgcc
     9361 tcgggccccg tgatcagccg gtcttcgccg atcatgcggt tgatcagcgt cgacttgccc
     9421 gcattgggac ggccgacgat cgccagcttc atcgggccga gcggcgcgtc ctcatcctct
     9481 tcgccgtcca cgagcggcag ggcgcccgct tcctcggcat catgactctc gacgatcggg
     9541 cggagcgcgt cgaacaggtc gaccacgcct tcgccatgtt cggcgctgag cgcgatcgga
     9601 ttgtcgaaac cgagcgaata gctctccatc aggccgtttt cgccctgctt cccctccgcc
     9661 ttgttgacga ggaggatgat cggcgtgtcc tcgctgcgca gccagcgcgc gatttcctcg
     9721 tcgagcgggg tcacgcccgc gcggccgtcg atcatgaaca gcgcggcatc ggcctcgcgc
     9781 accgcctttt cggtctgcac gcgcatccgg ccgggcaggg tcgccgcgtc ttgatcctcg
     9841 aaccccgcgg tgtcgacgat ccggaattcg agcccgagca gcttggcatc gccctcgcgc
     9901 cggtcgcgcg tcacccccgg ctggtcgtcg acgagcgcga ggcgcttgcc gacgagccgg
     9961 ttgaacagcg tcgatttgcc gacattgggc cgcccgacaa tggcaatcgt cgcgtgtcgc
    10021 gacatcagtt cattcctaca aagccctctc cccttcaggg gagaggatag cgaagcttgc
    10081 tccggcagga gctagcggag cttggagagg ggcgaagctt tcatggcccg caccccccct
    10141 ctccaactcc ggctagggcg ctcgcgccca agccttcata tcctctcccc tgaagggaga
    10201 gggaaagctt agtcaatcag cgccaggccg tgatcgtccc gtcgtccgcg aggacgtaca
    10261 agatgttgtt cgcgacgacc ggggactgcg acagcgacga atcgaattcg gtcgaggcga
    10321 gcagcgaccc gtcggtcggc gaatattccg ccagcgtccc ttcgctgttg accgcgatca
    10381 gccgcccgcc ggccaggatc ggccctgtcc agcggatcgg gtccttcttc ttcttttcgg
    10441 tttcgatgcg gaagcgcgcg agctgctgca gccagcggac cttgcccgac gaacgcgcga
    10501 cgcagagcag cttggcgtcg tcggtcatcg catagaccca ctcgccgacc acataggggg
    10561 tcgaaatacc ggcgatcgag atttcccagc tgcgctggcc ggtcacgagc tcatagctcg
    10621 ccatgcggcc gccctggccg agcgcgaaga catggccgcg gtcgatgacc gggtcggcat
    10681 cgacgtcggt gagcgtcgac accgacagcg ccatcgacgt gcgcgcgagc gcgtcttccc
    10741 acaggtcgcg gccattctcg taacgatagg cctgcacttc gcccgacgag aagccggcaa
    10801 cgatcgtccc ctgccccgcc gcaggcgacg ccgcgccgaa cacgctgccg gcctcggtcg
    10861 atgccgtcgc ctgccactgc accgcgccat tggcggcgct cagcgcgtaa atctggttgt
    10921 cctggctgat cacatagacg ccgccaaagg caatcgtcgg tgcgccgcgc agcggaccgg
    10981 tcggcttcac cttccagatc accgagccgt cggcggcgtt gagcgccgcg acgtcgccga
    11041 caccgctcgt cgcatagacg acattgccgt cgaccgcggc gccaccgccg aacagcgaat
    11101 ccttgaaatc cttgccggtg ctgccgatcg ccgcgctcca cagcttcgcg ccggtatcgg
    11161 cggcaaaggc cgaaacgacg gcatcggtat cgacgacgaa cagccggttg tcggcgacca
    11221 ctggcgacga ggcgaggcgc tgcttgttgg tgctgcccgc gatgctcgct tcccacagct
    11281 tcgctcgcgt cgcgcccagc gccggatggc ccatcgattt cgatgcgttg ccgcccgact
    11341 gcgcccaggc cgcgttcacc gcgggctcgg ggagcacgac cgccatcgcg gcggtggccg
    11401 ggtcgacctt gatgctgttg tcgttcgaca ggatcgatac gcgctcgccg accgtcgggg
    11461 tcttcggcgg accgtcgccc ttgaacaccc cgcacgccgc aagcggcaac gccagcagcg
    11521 tcgcataaag tccgtagcgc gcgctgcgca taatatctgg ccgcatatta attggctttc
    11581 tcagtcttgc tggctgccgc tgccttgggc gcggcgttgc ctttctcttc ggcggcggcg
    11641 ctttccgccg cccggtcggt aaccgcatcg acgccgagca tgcccgccat ctgcaccgaa
    11701 cgcgattgca gcgaccgcgt cacgtcgggc gatttggcga tgcggccata gagcgcgccg
    11761 gcctggtcga actggccgag ctgataatgc gccgtcgccg acagctcggc ggcgctcgcg
    11821 aaccagctcg acgccggatc cttggcatcg accatcggct tcatgcgcgc gatcaccacc
    11881 tcgggcttca gcgtgtcata ttcgaaggcg gtctggcgga tcagcgcgag gtcgcgcagc
    11941 gccttgtcga gtttggtgtc ggcggcgacc ttggccatca gggcggcagc gcccttgagg
    12001 tcgccggtct gcgccttgat gttcgcttcc tgcatctgcg cgaccgcgcg ataggcgggg
    12061 ccgccctcgg cggcgatctt ctgcaattcg gcggtcgcgg cgcgcggctg attggtggcg
    12121 agcttttcga acgccgcgat cagttcctcg gcctgttcgc cgcgcgcggc atcctgccga
    12181 tggtcccaga aaagatagcc gccgaacgcc agcagcgcgg ccagcacgcc gccgatgatc
    12241 caccggccgt aacgctgcat gatcgtgtcg agccggtcct tgcggacggc ttcgtcgact
    12301 tcctgcaaca gcgcggcgtc attcgtcggg ctcagggcca atcaaacctc cataaactca
    12361 atgcggcgct gggccggccc gcttccttag cgatggcacg gggaaaggcc aagcgtttca
    12421 ctttgggtgg tcgccgcgcc gtcagccggc gccatcaacc cgcgtaggtc tgatcctcgg
    12481 tcgggaatga acgggacctg acttcgtcgg catagtcctt gaccgcgctt tcgacgacgc
    12541 cggccatatt cccgaaacgc ttcacgaatt tgggaacgcg ttcgaacatg ccgagcatgt
    12601 catcggtgac gagcacctgg ccatcgcact ccgccgaagc accgatgccg atcgtcgggc
    12661 aatcgacctt gttcgtgatc tcgatcgcga tcgattcgag cacgccttcg atgacgatcg
    12721 aaaaggcgcc ggcctgcgcg acggcgacgg cgtcctcgac gatcgagcgc gcttcctccg
    12781 cacttttgcc gcgcacgcca tagccgccga gaatattgac cgcctgcggc gtcagcccga
    12841 catggcccat caccggaatg ccgcgctggg tcaggaattc gatcgtcggc gcgagcacct
    12901 tgccgccctc gaccttcacc gccgcggcgc cggtttcctt gagcagccgc gcggcattgt
    12961 cgaacgcctg ctcggggctg ccctcatagc tgccgaacgg catgtcgacg atcaccgcgg
    13021 catgatagct gccgcgcacg acggcggcgc cgtgcagcgc catcatgtcc atcgtcacgc
    13081 cgactgtatg cggcagacca tagatcacct gcgcgagcga atcgccgacg agcagcatgt
    13141 cgcaatgcgg atcgagcagc tgcgccatgc ggacggtgta tgcggtcagc atcacgagcg
    13201 gctcgccgcc cttgcgctgg cggatgcgcg gaaccgtcag gcgcttcatc ggctgcggcg
    13261 tcgggttggc gcggctggtc gaggtgtcga gggtgagcgt tttggggagc gtggacatgg
    13321 cgcgagggtt tagccgctaa acgggcggcg cgaaagcgcg aaagcccgtt tttgttgcag
    13381 cgcagcgtgg gcgcgtcagg ccccgctcag cgccgccagt tgctgatcgt tcaggtcgag
    13441 atcgaggctg cccatcaatt cgtcgagttg cccgaccgtc gtcgcactcg cgatcggcgc
    13501 cgttacgccg ggctgcgcga cgacccaggc gagcgcgatc tgcgacagcg tcgcgccggt
    13561 ctccgcggcg atcgcgtcca tcaccgccag aaccgctggc ccccggcccg ccatataggc
    13621 tttcgcctta tagccccgcg cgctctgtcc cagatcttcg gcgccgcggt atttgccggt
    13681 cagataaccg gcggcgaggc tgtaataggg aatgactccg atctcctcgg cgatgcacag
    13741 ttcctgcagc gggccttcat attggtcgcg gtcgagcagg ttgagctcgg gctgcatcac
    13801 ggtgaagcgt ggcagccgct tttccgccga cacgcgcaat gcttccgcga gccgctcggc
    13861 cgaatagttg gacgcgccga tcgcgcgcac tttgcccgcg tcgaccaact cgccgaacgc
    13921 gccgagcgtt tcgtcgagcg gcacgtcggt gtcgtccttg tgcgcgaaat agaggtcgat
    13981 gacatcgacc ccgagccggt cgagcgaacc ctgcaccgcc gcgtggatgc ggtcgggtct
    14041 cagcccgccc tcgcccattc caaccttggt cgcgatcagc acctggtcgc gcgcaccgct
    14101 cgctttcagc cattcgccga tcattgcttc ggattcgccg cccttgtgcc ccggaaccca
    14161 cgccgagtag acgtcggcgg tgtcgatcat gccgcccccc tgctcgacga agcgatcgaa
    14221 gatcgcgaag ctcgcggtgc ggtccgcggt ccatccgaac acattgccgc caagcacgaa
    14281 ggggcggatc gacaggccgc tttggtccag agcatgttcg gtcatttcga agctcctttt
    14341 gcaaccgcgg ccaccgcctc gcgcgggttg tcgctgaaca ggccgtcgac gccggcggtg
    14401 agataggcgt cgatctcgcc cgccagatcg ccatggcccg cgggattgat actggccttg
    14461 tcgccgagcg gcaggaagta attttcgcgg cgaaacgtcc agggatgcac tttgagcccc
    14521 gcggcgtggg cgtcgcgcac cagcgcggtc ggcttgccga gccggccgag cgttccgcgc
    14581 gggatcacca tcgccttgtt cggaccgatg ccgtcggcat agccggcgat catcttgagc
    14641 cccgcgggcg acgccatggc ggcatagctg gtgcccggcc ggtcggcggg cccgccgccc
    14701 gcatccatca actggatcag cggcagatcg ctcttcgcgc gcaggtcgat cagattgccg
    14761 acctcgaagc tctggatgaa caccggcgcg gtgcggccgc gatagccgaa acgatcgagc
    14821 atcgcgagca gcggcgcttc atgcggcagg ccgatcgcga cgaaatagcc cggatgcttg
    14881 gtttcgggat agacgcccac gggccggtcg cgatccttgt tcacctcggc gagcagcgtc
    14941 aaaatctcct cgaaggtcgg aatttcgaac tgtccgtcat agtcggtgct gcgcagccgc
    15001 gggaggcgct cacgcgcgcg cagcgtcttc agttcggcga gcgtgaaatc ctcggtgaac
    15061 cagccctcga ccttttgccc gtcgatcgtc ttctcgatct tgcggcccgc gaactcggga
    15121 tggtcggcga cgtcggtggt ctccgaaatc tcattctcgt gctgcgcgac gaggattccg
    15181 tccttggtca gcaccagatc gggctcgatg aaatcggcgc cctgctcgat cgcgagccgg
    15241 tagctcgcca gcgtgtgctc ggggcgttcg cccgatgcgc cgcgatgcgc gatcacgatc
    15301 ggcggccgtc cgtcgagggt gggctgcgcg gtcacgtcgt cagccgtgca gccagtggac
    15361 agcatcagcg ccgccacggc cgcgatcgac cggatcatgc ggcaaaccgc gcctgccagt
    15421 ccgcggccga aaatccgaca ctgatatcgc cgccgttttc gacgaccggg cgccggatca
    15481 tcgccggttg ctcgagcatc agcgccttgg ccgacgccgc atcgagcccc tctttctgct
    15541 cttcgggcag cttgcgaaag gtcgtcccgg ccttgttgag cagcttttcc cagccgagcg
    15601 catcgatcca gccctgcaac cgtgccgcgt caagcccgtc cttgcgaacg tcgtggaagc
    15661 ggtaggcgac gccccgctcg tcgagccagc gccgcgcctt cttcaacgtg tcgcagttcg
    15721 aaatgccata gagaatcatc gtcatgcgac ggggattagc aggcaggggt tgcagattcg
    15781 aggccgcgcg gacgcgcgcc cggaaaaatc gctcatcgct cggcgatacg cgtgttggcg
    15841 ctggccagct tgccgcgcag cgcgcgggcg aagccctcgt ggagcgcgcc cgcgatcgcc
    15901 ggattggccg tgagataggc gcgcagcgcc gccgcaggga cgaaccacag acgcgcattg
    15961 ctgccgagca cgaccgtgcc cgtcgcatgg ctaccgtcga gcgcggtcgc ttcaccgatc
    16021 agcgcgccgc ccgataattt cccgacctcg acgccgtcgc gcaggaccgc cgcctggccc
    16081 tcggcaagat agaagaggct cggcgccgcc tgattctcgc ggatcagcac ttcgccgcgc
    16141 ttcgccgaaa tccagtgccc ctggtcgatc aagtcgcgcg ccgctactgt ccccagcccg
    16201 ttgaaatact cgcggcgcag cccctcttcc tcggccgaaa aacgcacctt cgtcccgcgc
    16261 agccacaggc gcgccaatat gccgatattg accgccaata tcgcgagcac gagcaggctg
    16321 tatccaaggt ccgccccgcg aaagagcgtc aatggcaatg ccaagagggc tgccgcggcg
    16381 aggccgatgc gcgcatagtc gatgcggacc acgaaactcg tcgccaaaag gagcaacagc
    16441 gctccgtaaa ggaaccaggc ggaatcgaaa ttcgtcacag ccaatcgctc gcatcccgac
    16501 ccgccccacg caccggggta tcaggtcact gggtggttga cgttaccgat agccaaaagc
    16561 cttggctgcg cgcgcccatc gccgctccaa ggcccgtaat aaactatcag ttcccggccc
    16621 tcaagaaaag cataggtgcg caaatgtcac gataatgcta attttccggt ttgcccgttc
    16681 cggaaacatt gcttcgggcg ggcatccgcg ccatggggcg caggattcga aagcgaaaag
    16741 acaagcagat gacgaaaaat tggcaaccgc atagctggcg ttcgcatgag gcccgccagc
    16801 ttcccaccta tcgcgacgcc gatgcgctcg cggccgccga acgcgagctc gcaaattatc
    16861 cgccgctcgt cttcgcgggc gaagcgcgcg agctgacgag cgaactcggg cgcgtcgccg
    16921 aggggaaggc cttcctgctt cagggcggcg actgcgccga aagctttgcc gaatttcatc
    16981 cgaacaatat ccgcgacacc ttccgcgtcc tgctccagat ggcggtcgtg ctgaccttcg
    17041 cgtcgaagat gcccgtggtg aagctcggcc gcatggcggg ccagttcgcc aagccgcgct
    17101 cggccgatct ggaagatatc gatggcgtcg aactgccgag ctatcgcggc gacatcatca
    17161 acgacatcgc gttcgaggaa gcgggccgcg agcccgaccc gcagcgcatg gtcaaggcgt
    17221 ataaccagtc ggcggcgacg ctgaacctgc tgcgcgcctt cgccaacggc ggctatgcga
    17281 acctgcatca ggtcaacgcc tggacgcacg atttcatgga ccgcagcccg tgggcgaaga
    17341 aatatcagga aaccgccgcg cggatttccg aagcgctcgc cttcatggaa gcgtgcggag
    17401 tgacccccga aaccgtgccg cagatcaagg gcaccagctt ctacaccagc catgaggctt
    17461 tgctgcttcc ctatgagcag gcgctcacgc ggcaggacag tctgaccggc ggctggtacg
    17521 acacctcggg ccatttcctg tgggtcggcg accgcacccg cttcgaggga tcggcgcaca
    17581 tcgaatatct gcgtggcatc ggcaatccgg tcggcatgaa atgcggcccg agcctcgagc
    17641 ccgacgcgct gctgcgcctg ctcgacacgc tcaatccgaa ccatgtcgcg ggccgcatga
    17701 cgctgatcac gcgctatggc cacgacaaga tcgaggcgca tctgcctaag ctcgtccgcg
    17761 cggtgaagga atcgggtcat cccgtcgtct ggtcgtgcga cccgatgcac ggcaatgtca
    17821 tcaagacctc gaccggctac aagacgcgcc cgttcgagcg catcctcgcc gaagtgcgcg
    17881 gcttcttcgc cgtgcaccgc gccgagggca cgcatggcgg cggcatccat atcgagatga
    17941 ccggccagaa tgtcaccgaa tgcaccggcg gcgcgatgga cgtgacccag atggatctgg
    18001 ccgaccgcta tcacacgcat tgcgacccgc gtttgaacgc ggggcaatcg ctggaactcg
    18061 cattcctgct ggcagagatg ctcaatcagg aaatggccga ccgcgcgaag caggcggcct
    18121 gattattccg tcgcccccgc gaaggcgggg gcgacgctat tacttccccg gccaccgctt
    18181 ggtatcggcg aacgcccgcc ccagcgcgac gtgcaggtcc gcatcctccg cagcgccgcc
    18241 gagcgggatc ttgtcggaga aattgtccgc cgcctgatga tagtcgctgc cgaggaaggc
    18301 ttcgagcagc ttcatgtccg aaaagctccc cccgaccatc agcgacgtca cgcccttggc
    18361 gccgagcgcc cagccgtcct gccgctggat gaaggcgtcg gcctcgccat cctcgtcgag
    18421 cttgcggccg agcttggtcg caacctcgcg caccaccgcg tcataggcgg gcttgccgcg
    18481 cccgatcgtc gccaccggcg tgccgcgcgg cgagatcgcg atcgtgtcga cgttgagcgc
    18541 gacggtgatg tcggacagcg gcaccaccga atgatcggcg aaataatgcg cgccgagcag
    18601 acccttttcc tccgccgtcg tcgccatgaa ataaatgtcg cggtcgggcc gcgtgccgtt
    18661 gcccagccgc ttcgcgacct cgatcagcac cgcgatgccg ctcgcattgt cgaccgcgcc
    18721 gttgcagatg cggtcggcgg cgccttcggg ttcgcagatc ccgagatgat cccagtggcc
    18781 gaggaacagc accgccttgc cgtcgggctt ggcgccgggg agcttggcga tgatattgtg
    18841 gctggcaaag gcacggacgg ccgaccctgc ggtaaagtcc gccgtcaccg gcagcgcggc
    18901 gcccttgtaa tcgggcgact tggcggcctc gcgcagcgcg gcgccgtcct gtcccgccgc
    18961 cttgagcagc gtgccggcgg cttcgg
//
LOCUS       DATMSU010000270         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_42886, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000270 DATMSU010000000
VERSION     DATMSU010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42886"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VN034_07325"
     CDS             complement(<1..606)
                     /locus_tag="VN034_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWV60445.1"
                     /translation="MRLSRRNFLDAATLLALSSPLAARAAVKGKAAPVTQDAVAMAQA
                     VRDGRTTPRALVEAAITRLEKANPQVNCVALPNFERALEQAEKGVTGPLAGVPTLIKD
                     NIDQKGLPNTNALKLFQSNIAAKNDPYVDAIERAGLISIGRSTLPELASNVTTESALT
                     GITRNPWNPDYTSGGSSGGASAAVAAGVVPIAHGNDYAGSIR"
     gene            complement(653..1153)
                     /locus_tag="VN034_07330"
     CDS             complement(653..1153)
                     /locus_tag="VN034_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HWV60446.1"
                     /translation="MTASRIMLMLVAAQALFSPALASEPAAGARQRALVTLPDDPEAR
                     RAREDWLYADAVLSGDTAYLSGIIALPAEGDPSPEAAFDKAFGRLARRLARVGCSWDD
                     VVEMTSFHTDIDAQLPAFKAMMRKYIRPPYLAWTAIGVSRLAVPSGVAEIKLVAKDCT
                     GRNPGS"
     gene            complement(1179..>2486)
                     /locus_tag="VN034_07335"
     CDS             complement(1179..>2486)
                     /locus_tag="VN034_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWV60447.1"
                     /translation="MVAIALPNGPAFHAAAFAAWKCGATPAVLSPRLPAHEYAALIDL
                     IQPRLVVGSETEGAADARAEGESQASEVATYWKAMCSGGSTGRPKIIVDHMRGEYDPA
                     GPGLHNLLQIPNDGVLLNPGPLYHNGPFLFSSLALFAGCTVVGMERFDAEETLRLIEK
                     HRVEWISLVPTMMHRIWSLPAEVRERYDLSSLKVMLHMAAPCPPWLKEAWIDWLGGER
                     IWEIYAGTEGSGATFIRGDEWLERRGSVGRIAGASRIKAVREDGSTCAPGEIGELYFM
                     PEGGRATSHYMGADEKRSDDGWLSIGDLGHLDRDGYVYLADRRTDLIIRGGANIFPAE
                     VEAAIEAHPAVVNAIVVGLPCEEMGQRVHAIVEHPTDTALALEDLGAFLAERLVKYKM
                     PESFEFTTTALRDESGKARRSALRDERIDWLGQGRHFKAGAAA"
BASE COUNT          388 a          802 c          855 g          441 t
ORIGIN      
        1 gcggatcgac cccgcatagt cgttgccgtg cgcgatcggc accacgcccg cggcgaccgc
       61 ggccgacgca ccgcccgatg agccgcccga tgtataatcg gggttccacg gattacgcgt
      121 gatccccgtc agcgcggatt ccgtggtgac gttgctcgcg agctcgggca gcgtcgaacg
      181 tccgatcgag atcagcccgg cacgctcgat cgcgtccaca tagggatcgt tcttcgccgc
      241 gatattggac tggaacagct tcagcgcatt ggtgttgggc agacccttct ggtcgatatt
      301 atccttgatt agcgtcggca cgcccgccag cggcccggtg acgcccttct cggcctgttc
      361 gagcgctctt tcaaaattgg ggagggcgac gcaattcact tgcggatttg ctttttcgag
      421 gcgtgtgatc gctgcctcga cgagcgcgcg tggggtcgtt cggccgtcgc gcaccgcctg
      481 cgccatcgcg acggcatcct gggtaaccgg cgcggccttg ccctttaccg cggcacgcgc
      541 ggcgagcggc gaggacagcg cgagcagcgt ggcggcgtcg aggaagttcc ggcgtgacag
      601 gcgcatcaga tagctccgtg gatgcgggga atgatgggat gatcgttcgg gttcacgatc
      661 cggggttccg gcccgtacag tctttcgcga ccagcttgat ttcggcgacg cccgacggga
      721 cggcgaggcg gctgacgccg atcgcggtcc aggcgaggta gggcgggcgg atatatttgc
      781 gcatcatcgc cttgaaggcg ggaagctgcg catcgatgtc ggtgtgaaag cttgtcatct
      841 cgacgacgtc gtcccaactg cagccgaccc gcgcgagccg tcgggcgagg cggccgaacg
      901 ccttgtcgaa cgcggcctcg ggcgacggat cgccctccgc gggaagcgcg atgatcccgg
      961 acagataagc ggtgtcaccc gacagcacgg cgtcggcata gagccagtcc tcgcgtgcgc
     1021 ggcgcgcttc gggatcgtcg ggaagcgtca cgagcgcgcg ctgccgggcg ccggctgcgg
     1081 gttcgctggc aagcgcgggc gagaacagcg cctgcgcggc gacgagcata agcatgatgc
     1141 ggcttgcggt catttcgccc ccttcgcccc gatttccatc aggcggccgc gccggccttg
     1201 aaatgcctac cttgtccaag ccagtcgatc cgttcgtcgc ggagcgccga gcggcgcgcc
     1261 ttgcccgatt catcgcgcag cgcggtggtc gtgaattcga aggattcggg catcttgtat
     1321 ttgacgaggc gctccgcgag gaaggcgccg aggtcttcga gcgccagtgc ggtgtccgtc
     1381 ggatgttcga cgatcgcgtg aacgcgctgc cccatttcct cgcacggcaa accgacgaca
     1441 atcgcgttga cgacggcggg atgcgcttcg atcgcggctt cgacctcggc gggaaagatg
     1501 ttggcgccgc cgcggatgat gaggtcggtg cgccgatctg cgagatagac atagccgtcc
     1561 ctgtcgagat ggccgaggtc gccgatcgac agccagccgt cgtcgctgcg cttttcgtct
     1621 gcgcccatat agtgtgacgt cgcccgcccg ccttcgggca tgaagtaaag ttcgccgatt
     1681 tcgccgggcg cgcaggtcga cccatcctcg cgcaccgcct tgatccggct cgcgcccgcg
     1741 atccggccga ccgaaccgcg acgttcgagc cattcgtcgc cgcggatgaa ggtcgcgccg
     1801 ctgccttcgg tgcccgcgta gatttcccag attctctcgc cgccgagcca gtcgatccac
     1861 gcctccttga gccagggcgg gcagggggcg gccatgtgca gcatgacctt cagcgacgag
     1921 aggtcgtaac gctcgcggac ctcggcgggc agcgaccaga ttcggtgcat catcgtcggg
     1981 acgaggctga tccactcgac gcggtgtttc tcgatcaggc gcagcgtttc ctcggcgtcg
     2041 aagcgttcca tgccgacgac ggtgcagccg gcgaaaagcg cgaggctgga aaagaggaag
     2101 gggccgttgt gatagagcgg gcccgggttg agcagcacgc cgtcgttcgg gatctgcagc
     2161 agattgtgga gacccggtcc tgccgggtca tattcgccgc gcatatggtc gacgatgatc
     2221 ttcggccgcc cggtcgagcc gccgctgcac atcgctttcc aataggtcgc gacctcggaa
     2281 gcctgagact ccccctccgc ccgcgcatct gcggcgcctt cggtttcgct gccgacgacg
     2341 aggcgcggct ggatcaggtc gatgagcgcc gcatattcat gcgcgggcag gcgcggcgaa
     2401 aggaccgccg gcgtcgcgcc gcatttccac gccgcgaagg cggcggcgtg aaatgccggg
     2461 ccgttcggga gcgcgattgc caccat
//
LOCUS       DATMSU010000271         5629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_156545,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000271 DATMSU010000000
VERSION     DATMSU010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156545"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1392
                     /locus_tag="VN034_07340"
     CDS             346..1392
                     /locus_tag="VN034_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60448.1"
                     /translation="MVTRAIISFDTELSAGLYQRGHDARANFESSILGRCRDGDFGIH
                     FQMDMLERYGLKGVFFVDPMPALVHGPAIIRDIVAPIVARGHEVQLHIHTEWLAFARF
                     NPVGRLSGRNIGDFPLGAQKKLIGLAADMLVRAGAPDPVAFRAGNFGANDDTLRALDA
                     LGFLFDSSFNGAHVGHGCDIALDSRNIGMRLHHGVCEVPVSGLMDRADSFRPAQLCAM
                     SEEEMRDALDHAAASGAIQFSAFSHSFELLSRDRAVPNRLAIARMEALCQAVVADPRV
                     TNGGFADLPQPPARPGPIRPPEPRPLRTLRRTAEQAAGHLAHEVRYVRSLAYVTANTS
                     RWGKIAGGILLAMA"
     gene            complement(1345..2484)
                     /gene="zapE"
                     /locus_tag="VN034_07345"
     CDS             complement(1345..2484)
                     /gene="zapE"
                     /locus_tag="VN034_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HWV60449.1"
                     /translation="MTTVLAAYDALVAAGELRPDPEQRAAATRLNQLQAELEAVPKRG
                     SLLWRLAGRKPEALRGVYLWGAVGRGKSMLMDLFYDQLAIQRKRRVHFHAFMLDVHAR
                     MREVRKSESGDPIPLVADALAENTRCLAFDEMVVNNSADAMILSRLFTALIDHGVTMV
                     ATSNRPPKDLYKDGLNREHFLPFIALVEERLDVMGLNGPTDYRRDRLGDGARWFVPAD
                     DAASAALSAAFFRLTDYPPEDRAHVPSLDLDVGGGRTLHVPKALKGVAVFSFKRLCGE
                     ARGAADYLAVARHFHAVIIVGIPRMGPENRNEAARFVTLIDALYEYKVKLLASAAAMP
                     DQLYVAGDGAFEFERTASRLAEMQSDDYLALGHGQQDAAGDLPPT"
     gene            complement(2481..2972)
                     /locus_tag="VN034_07350"
     CDS             complement(2481..2972)
                     /locus_tag="VN034_07350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWV60450.1"
                     /translation="MNDGIEEPKRRENFSAEQLAEGWVSWNLKDQSRFNAFIEPLSVR
                     AEPPTDDGRPRARVRMLPERKHSNLGDNVHGAVSLALVDIALFAASHQFGSLDAGHSV
                     TLDLSTQFVGAGRVGEPLDAVVELVRETGRLIFLRGLVVQGEGDSHIVLSFAGTIRKA
                     SAK"
     gene            complement(2969..3964)
                     /locus_tag="VN034_07355"
     CDS             complement(2969..3964)
                     /locus_tag="VN034_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWV60451.1"
                     /translation="MRGALAALVALALAAPASIASARSDPSPQSSSQPVVVAIGGDSW
                     LIPGRFPEGQSPDGNSVILKGSEGLILVDTGRRAGHVGSIMGAVFGLGDARPVAIVNT
                     HWHLDHISGNPMLRHSWPRIKVYGTSAIDGALTGFLANSAAASRKALEAGTIPATAVA
                     DVKGDLATIERGQELRPDVLVDEPSDMAIAGRPLSLRVAHHAVTERDLWIYDAAEKRA
                     IVGDLVTVPVPFLDTACPDGWLTALDEVAASGAEQIVPGHGPIMTITDFNRWRGAFGD
                     FIACAKGASALETCSAGWAAGAAKWIGDDTVRAKAMADYYGELVRSKKLDGYCRA"
     gene            complement(3964..4746)
                     /locus_tag="VN034_07360"
     CDS             complement(3964..4746)
                     /locus_tag="VN034_07360"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HWV60452.1"
                     /translation="MAQFVLPKNSRPQKTGTVHKAEGATAVKKFKVYRYDPDKGQNPH
                     FDTFEIDTEKCGPMVLDALIKMKSEQDSSLTFRRSCREGICGSCSMNMNGKNGLACTT
                     AIEDLKGDITITPLPSMDVIKDLVPDFTHFYAQYASIEPWLKTKTTTPSGKERLQSPE
                     EREKLDGLYECILCACCSTSCPSYWWNSDKFLGPAILLQAYRWLADSRDELTGERLDE
                     LEDPFRLYRCHTIMNCANACPKGLSPARAIAEIKKLEAERHV"
     gene            4865..>5629
                     /locus_tag="VN034_07365"
     CDS             4865..>5629
                     /locus_tag="VN034_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWV60453.1"
                     /translation="MSESAAQPAVTSRSVARGLGTTVLARLGAVVEIVAQPLYVLMFG
                     LAGYGLYAVLWAAVNLLENIFDLGMTSAMQRTVPQSASNAEAAAALRTAMIFGVGPCI
                     LVAALIAVFAAELGPLLNVAAKDRELVTPAIRIFVWALPLWAFVEIATSALRARMVFG
                     AEIRLRIVWEQVMRLVFAGLFFAGGLGLKGLFIAHLCSLGITAALSIRLLARHYSFAD
                     LWRGPWAGETARNTFLAGLSILPSNIIARLFGDVPAL"
BASE COUNT          940 a         1869 c         1821 g          999 t
ORIGIN      
        1 ttgcgtccca tggcgcgcgg cactcccttc ctggcaagcc cggcaattgg ttcacagacc
       61 ggcgggagca cgggctaaag ctccgctccg gtcccaagac tggcgacgcc cctacgccga
      121 ttcccttaac gaaacaaccg ccaaaaggac gaaaaaccgc ctttctttga taatagttcg
      181 caataacaat aagctttccg aaaaaggatt tcgaggacag gctggcgcgt gatatgacaa
      241 gatcgcaacg ggttcgcggt ggaatggaac aaaaatgcgt cgtccgcata ggggtatcgc
      301 cgccccgcga ttaaccctcc cttattccgg ctttgttacg cgaccatggt gacgcgcgcg
      361 atcatcagct tcgacaccga attgtcggcc ggcctctatc aacggggcca tgacgcgcgc
      421 gccaatttcg aaagctcgat cctcggccgc tgccgcgacg gcgatttcgg catccatttc
      481 cagatggaca tgctggaaag atacggtctc aagggggtgt tcttcgtcga cccgatgccg
      541 gcgctcgtcc acggacccgc gatcatccgc gatatcgtcg cccccatcgt cgcgcgcggt
      601 cacgaggtgc aattgcacat ccacaccgaa tggctcgcct tcgcgcggtt caatccggtc
      661 gggcggctca gcgggcgcaa catcggcgac ttcccgcttg gggcgcagaa aaagctgatc
      721 ggcctcgcgg ctgacatgct ggtgcgcgcc ggcgcacccg accccgtcgc ctttcgtgcc
      781 ggcaatttcg gcgccaacga cgacacgcta cgcgcgctgg acgcgctcgg ctttcttttc
      841 gacagcagtt tcaacggcgc ccatgtgggc catggctgcg acatcgcgct cgacagccgc
      901 aatatcggga tgcgcctgca tcacggcgtc tgcgaggtgc cggtcagcgg cctgatggac
      961 cgcgccgaca gctttcgtcc ggcgcagctg tgcgcgatgt ccgaagagga aatgcgcgac
     1021 gcgctcgacc atgcggcggc gagcggcgcg atccagttct cggccttcag ccatagtttc
     1081 gaactcttga gccgcgatcg cgcggtcccc aaccggctgg cgatcgcgcg catggaggcc
     1141 ttatgccaag cggtggtcgc cgacccgcgt gtgaccaacg gcggcttcgc cgacctgccc
     1201 cagcccccgg cacggccggg accgatcaga ccacccgaac cgcgaccgct caggacattg
     1261 cgccgcaccg ccgaacaggc ggcggggcat ctcgctcacg aagtgcgcta tgtccgcagc
     1321 ctcgcctatg tcaccgccaa cacgtcacgt tgggggaaga tcgccggcgg catcctgttg
     1381 gccatggcct aacgccagat aatcgtccga ctgcatttcg gcgaggcggc tcgccgtgcg
     1441 ctcgaattcg aaagcgccgt cgcccgcgac gtaaagctgg tcgggcatcg ccgccgcgct
     1501 cgccagcagc ttgaccttat attcgtagag cgcgtcgatc aacgtgacga agcgcgccgc
     1561 ctcgttgcgg ttctcggggc ccatgcgcgg gatgccgacg atgatcacgg cgtgaaaatg
     1621 gcgtgcgacg gcgagataat cggctgcccc ccgcgcctcg ccgcagagcc gcttgaacga
     1681 aaagacggca acgcccttca gcgccttggg aacgtgcaag gtccgtccgc cgccgacgtc
     1741 gaggtcaagg ctcggcacat gcgcgcggtc ctcgggcgga tagtcggtga ggcgaaagaa
     1801 ggcggccgac agcgccgcgc tcgcggcatc gtcggcggga acgaaccagc gcgcgccgtc
     1861 gccgagccgg tcgcggcggt aatcggtggg cccgttcagc cccatcacgt cgagcctttc
     1921 ctcgacgagc gcgatgaagg gcaggaaatg ctcgcggttg agcccatcct tgtagagatc
     1981 cttcggcggc cggttcgacg tcgcgaccat cgtcacgcca tgatcgatca gcgcggtgaa
     2041 caggcgcgag aggatcatcg cgtcggcgct gttgttcacg accatctcgt cgaaggcgag
     2101 gcagcgggta ttttcggcga gcgcgtcggc gacgagcggg atcgggtcgc cgctctcgct
     2161 cttcctgacc tcgcgcatcc gcgcgtggac gtcgagcatg aaggcgtgga aatggacgcg
     2221 ccgcttccgc tggatggcga gctggtcgta gaacaggtcc atcagcatcg acttgccgcg
     2281 cccgaccgcg ccccacagat agacgccgcg cagcgcctcg ggcttgcgcc ccgcgaggcg
     2341 ccacagcagg cttccccgct tcggcaccgc ttcgagttcg gcctgcaatt gattcaaccg
     2401 cgtcgccgcc gcgcgctgct cgggatcggg gcgaagctcg cccgccgcga caagcgcgtc
     2461 ataggccgca aggacggtgg tcacttggcg ctcgccttgc ggatcgttcc ggcgaagctg
     2521 agcacaatat ggctgtcgcc ctcgccctgc acgacgagcc cgcgcaggaa gatcagccgg
     2581 ccggtctcgc gcaccagttc gaccacggca tcgagcggtt cgccgacgcg gcccgcgccg
     2641 acgaattgcg tcgacaggtc gagcgtcacc gaatgacccg catcgagcga tccgaactgg
     2701 tgcgacgcgg caaacagcgc gatgtcgacg agcgcgaggc taaccgcgcc gtggacattg
     2761 tcgccgaggt tgctgtgctt gcgttcgggc aacatgcgaa cccgcgcgcg cgggcggccg
     2821 tcgtcggtcg ggggctcggc gcgcaccgaa agcggctcga taaaggcgtt gaaccggctc
     2881 tgatccttga ggttccagct gacccagccc tcggccaatt gttcggcgct gaaattctcg
     2941 cgccgcttcg gctcctcgat cccgtcgttc atgccctgca atatccgtcc agtttcttcg
     3001 agcgaacgag ttcgccataa taatccgcca tcgccttcgc gcgcacggtg tcgtcgccga
     3061 tccatttggc ggctccggca gcccatccgg ccgaacaggt ctcaagcgcg ctggccccct
     3121 tggcgcaagc gataaaatcc ccgaatgcgc cgcgccagcg gttgaaatcg gtgattgtca
     3181 tgatcgggcc gtgtcccggc acgatctgct cggcgcccga cgcggcgacc tcatcgagcg
     3241 ccgtcagcca gccgtcggga caggcggtgt cgaggaaggg aacggggacg gtcaccagat
     3301 cgccgacgat cgcgcgcttc tccgccgcat cgtaaatcca gaggtcacgt tcggtcacgg
     3361 catgatgcgc gacgcgaagc gacagcggcc ggcccgctat tgccatgtcg ctcggctcat
     3421 cgacgagaac gtcgggacga agctcctgtc cacgttcgat tgtcgcaaga tcgcctttta
     3481 cgtcggcgac cgccgtcgcc gggatagtgc ccgcttcgag agccttgcgg gaggcggccg
     3541 cgctgttggc aagaaagcct gtcagcgccc cgtcgattgc cgacgtcccg taaaccttga
     3601 tccgcggcca gctgtgcctg agcatcgggt tgccgctgat atggtcgagg tgccagtgcg
     3661 tgttgacgat cgcgaccggg cgcgcgtcac ccagcccgaa gaccgcgccc atgatcgacc
     3721 cgacatggcc tgcgcggcgg ccggtatcga cgaggatcag accttcggag cctttcagga
     3781 tgacgctatt gccgtcgggc gactgtcctt ccggaaaacg ccccgggatc agccagctgt
     3841 caccgccgat cgccaccacc accggctgcg acgaggactg cggtgatgga tcagaccgcg
     3901 cgctagcgat cgatgcggga gccgccagcg ccagcgcaac aagtgccgcc agcgccccgc
     3961 gcatcagacg tgccgctcgg cttccagctt cttgatctcg gcgatcgcgc gcgccgggct
     4021 caggcccttg ggacacgcat tggcgcagtt catgatcgtg tggcagcgat aaaggcggaa
     4081 gggatcttcc agctcgtcga gccgttcgcc cgtcagctca tcgcggctgt cggcgagcca
     4141 gcgataggcc tggagcagga tcgcggggcc aaggaacttg tcgctgttcc accaatagct
     4201 cgggcagctc gtcgagcagc aggcgcagag aatgcactca tagaggccgt cgagcttttc
     4261 gcgttcctcg ggcgactgca gccgctcctt gccgctcggc gtcgtcgtct tggtcttgag
     4321 ccaaggttcg atcgacgcat attgcgcata gaaatgggtg aagtcgggga cgaggtcctt
     4381 gatcacatcc atcgacggca acggggtgat cgtgatgtcg cccttcagat cctcgatcgc
     4441 ggtggtgcag gcgagcccgt tcttgccgtt catgttcatc gaacagctgc cgcaaatgcc
     4501 ttcgcggcac gagcggcgga aggtcagcga cgaatcctgc tcgctcttca tcttgatcag
     4561 cgcgtcgagc accatcgggc cgcatttttc ggtatcgatc tcgaacgtgt cgaaatgcgg
     4621 attctgcccc ttgtcggggt cgtagcgata gactttgaat ttcttgacgg cggtcgcgcc
     4681 ctcggccttg tggaccgtgc ccgtcttttg cgggcggctg ttcttgggca ggacaaattg
     4741 ggccatatcg cgggattccc ttgtgcaatc gcgcgctgcc tagacctgat tgccgtgggc
     4801 ggcaaggggg tgaacgcgcg aaagccgctg gcagccgcgc gggcaaggcg ctagggcgac
     4861 gatgatgagc gaaagcgccg cccagcccgc cgttaccagc cgcagcgtcg cgcgcgggct
     4921 cggcacgacc gtgctggcgc gactgggcgc cgtggtcgaa atcgtcgcac agccgctcta
     4981 cgtcctgatg ttcgggctcg cgggttacgg cctctacgca gtgctgtggg cggcggtgaa
     5041 cctgctcgaa aatatcttcg acctcggcat gacgagcgcg atgcagcgca ccgtcccgca
     5101 atcggccagc aatgccgaag ccgcggcggc gctgcgcacg gcgatgatct tcggcgtcgg
     5161 accgtgcatc ctcgtcgcgg cgctcatcgc cgtctttgcc gccgaactcg ggccgctgct
     5221 caacgtcgcg gcgaaggatc gcgagcttgt cacgcctgcg atccgcatct tcgtctgggc
     5281 gctgccgctg tgggcctttg tcgagatcgc gacatcggcg ctgcgtgcgc gcatggtctt
     5341 cggcgccgag atccgcctgc ggatcgtgtg ggaacaggtg atgcggctcg tcttcgccgg
     5401 actgttcttt gcgggcggcc tcgggctcaa gggactgttc atcgcgcacc tctgctcgct
     5461 tggtatcacc gcggcgctca gcatccggct gctcgcgcgg cactatagct tcgccgacct
     5521 gtggcgcggg ccgtgggcgg gagagaccgc gcgcaacacc tttttggcag ggctttcgat
     5581 cctgccgtcg aacatcatcg cgcggctgtt cggcgacgtg ccggcgctg
//
LOCUS       DATMSU010000272         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_184779,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000272 DATMSU010000000
VERSION     DATMSU010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184779"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VN034_07370"
     CDS             complement(<1..688)
                     /locus_tag="VN034_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWV60454.1"
                     /translation="MDGPVADRGEARLFAGIELGGTKCICTLAAGPGEIRDQRTIATT
                     VPSETLPAIKAVLDDWARSPGFCSIGVASFGPIRVDPAKADYGRVGATNKPDWEGADL
                     LGPLKSAFSVPIGFDTDVNGAALAEIRWGSGRGLDDFAYVTVGTGVGVGLVVHGKPTR
                     GFSHSEIGHVLVPRLVGDDMVSVCRFHDDCVEGLASGTALKARLDGRSLADVAPDDPI
                     WEPIVHTLATM"
     gene            complement(875..>3139)
                     /locus_tag="VN034_07375"
     CDS             complement(875..>3139)
                     /locus_tag="VN034_07375"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HWV60455.1"
                     /translation="PAGCSRAAGEALAIGKPNLFIGTGGHGHTFPGASLPFGMAQLSP
                     DTNTHGWDACSGYHQKDGSIMGFSHTHLSGTGIGDMLDVLVVPTRRELKLEPGTIEKP
                     GEGFRQRYSDEHAEPGYYRVKLETGVLAELTVTERCGLHRYRFGQGAGHILIDFAHAI
                     EDDWDKGIVVENASLDLGDDGTLTGGRQVNRWAKGRRIHFAMQMSRRPDRVQFFGDDG
                     KPAPDGATSIKGNKLKVAFFFDDAGGPPILIKTGLSAVDVKGARDALAQEVPAWDFDG
                     AVKAARGTWAKALGGIRVSGGTEAQRVIMTSALYHAQLAPTLFTDRDGRYVGLDRQVH
                     EAAKGEEAFSTYSLWDTYRALHPLLTLIDPERAALLTRDICRQTAQSPAGPLVWPLQG
                     VETACMIGWHGVSVLAEAHAKGIEADYAAAWPNIRRRAFHRDYKDIDSTLGRGFYYDL
                     GYIPCDEVWESVSRTQEYAYDDWAMAHLADAVGAKEDAAALRKRAQNYRGVIDPETRF
                     ARPRFKDGSWWKDYDPVQIGHNVKKWRDYTEANGWQATFLNQHDVYGLIDHFGGDAAF
                     EKQLDALFNAPSTLPDNAPPDISGLVGQYAHGNEPNHHVAYMYAYCGAPAKAQAMVRR
                     LLTEMYKNDPDGVIGNDDCGQMSAWFILSALGFYPVDPVSAVYVFGSPLFDVAEVTVG
                     AGKTLTVRAEGNGPDHPYVQSVTWNGKPWSRNWIAHADLAAGGELVFEMGAKPSAFGT
                     AKADRPPSFDSAPA"
BASE COUNT          507 a         1024 c         1065 g          543 t
ORIGIN      
        1 ccatcgtcgc cagcgtgtgg acgatcggtt cccagatagg gtcgtcgggc gccacgtccg
       61 ccagcgatcg gccgtccagc cgcgccttca gcgcggtgcc ggaggcgagt ccctcaacgc
      121 aatcgtcgtg gaagcggcaa acgctcacca tatcgtcgcc gacgaggcgc ggcacgagaa
      181 catggccgat ctcgctgtgg ctgaacccgc gcgtcggctt gccatggacg accagcccga
      241 cgccgacccc ggtgccgacg gtcacatagg cgaaatcgtc gagcccgcgt ccgctgcccc
      301 agcggatttc ggcgagcgcc gcgccattga cgtcggtgtc gaaaccgatc gggaccgaaa
      361 aggccgattt gagcgggccg agcaggtcgg cgccttccca atcgggcttg ttcgtcgcgc
      421 cgacccggcc atagtcggcc ttggcgggat cgactcggat cgggccgaag gaggcgacgc
      481 cgatgctgca aaaaccgggc gaccgcgccc agtcgtcgag cacagccttg atcgccggca
      541 gcgtttcgga cggcacggtg gtcgcgatcg ttcgctgatc gcggatctcg cccgggcccg
      601 ccgcgagcgt gcaaatgcat ttggtgccgc cgagctcgat gccggcgaac aggcgcgctt
      661 cgccgcggtc tgctactgga ccatccatca gcgccacccc ttcgcccgat cgctcaggtt
      721 tttggagggg agcgggccgc cgcgcaaggc cgaagcccgg gctggcgcaa atatcgttcc
      781 gggtccggcg agcggcaggc tttggggaga tttcacgaga cattccgttc ttatcatttt
      841 cttttcgcta ccaaaaactt gtcgcaaatt tgcgtcatgc gggggcggaa tcgaaggaag
      901 gcggccggtc ggccttggcg gtgccgaacg cagacggctt ggcgcccatc tcgaagacga
      961 gctcgccgcc ggcggcgagg tcggcgtgcg cgatccagtt cctgctccac ggcttgccgt
     1021 tccaggtgac cgactgcacg taaggatgat cggggccatt gccctcggcg cggacggtga
     1081 gcgtcttgcc cgcgccgacc gtcacctcgg cgacgtcgaa caagggcgag ccgaagacat
     1141 agaccgcgct caccggatcg acgggataga agcccaatgc cgagaggatg aaccacgcgc
     1201 tcatctggcc gcaatcgtcg ttgccgatca ccccgtcggg gtcgttcttg tacatttcgg
     1261 tgagcaggcg ccggaccatc gcctgcgcct tcgcgggcgc gccgcaatag gcgtacatat
     1321 aggcgacgtg gtggttcggc tcgttgccgt gcgcatattg gccgacgagc cccgaaatgt
     1381 cgggcggcgc attgtcgggc agggtcgacg gcgcgttgaa caacgcgtcg agctgtttct
     1441 cgaacgccgc gtcgccgccg aaatgatcga tcaggccgta aacatcgtgc tggttgagga
     1501 aggtcgcctg ccagccgttc gcctcggtat agtcgcgcca tttcttgacg ttgtggccga
     1561 tctggacggg gtcataatcc ttccaccagc tgccgtcctt gaagcgcggg cgcgcgaagc
     1621 gcgtttcggg gtcgatgacg ccgcgataat tctgcgcgcg cttgcgcaag gccgcagcgt
     1681 cctccttggc gccgaccgcg tcggcgagat gcgccatcgc ccagtcgtca taagcatatt
     1741 cctgcgtccg gctgaccgat tcccacacct cgtcgcacgg aatgtagccg aggtcgtaat
     1801 agaagccgcg gcccaaagtg ctgtcgatat ccttgtaatc gcggtggaag gcgcggcggc
     1861 ggatattggg ccaggcggcg gcatagtcgg cctcgatgcc cttggcgtgc gcttcggcga
     1921 gcaccgacac gccgtgccag ccgatcatgc acgcggtctc gaccccctgc agcggccaga
     1981 cgagcgggcc ggcggggctc tgcgcggtct ggcggcagat gtcgcgggta aggagcgcgg
     2041 cgcgctcggg atcgatcagc gtcagcagcg gatggagcgc gcgataggtg tcccatagcg
     2101 aataggtgct gaacgcctcc tcgcccttcg cggcttcatg cacctgccgg tcgagcccga
     2161 cataacgccc gtcgcgatcg gtgaagagcg tcggcgcgag ttgcgcgtga tagagcgcgc
     2221 tcgtcatgat cacgcgctgc gcttcggtgc cgcccgacac gcggatgccg ccgagcgcct
     2281 tcgcccaggt gccgcgcgcg gccttgactg caccgtcgaa atcccatgcc ggaacctcct
     2341 gcgccagcgc atcgcgcgcg ccttttacgt cgaccgccga aaggccggtc ttgatcagga
     2401 tcggcgggcc gccggcgtcg tcgaagaaga aggcgacctt gagcttgttg cccttgatcg
     2461 aggtcgcgcc gtcgggggcg ggcttgccgt cgtcgccgaa gaattgcacg cggtcggggc
     2521 gtcgcgacat ctgcatcgcg aaatggatgc gccggccctt ggcccagcga ttgacctgcc
     2581 gcccgccggt gagcgtgccg tcgtcgccga ggtcgagcga cgcattttcg acgacgatgc
     2641 ccttgtccca gtcatcctcg atcgcgtgcg cgaaatcgat caggatatgg cccgcaccct
     2701 gcccgaaccg atagcggtgg aggccacaac gctcggtgac ggtgagttcg gcgagcacgc
     2761 cggtttcgag cttcacgcgg taatagccgg gctcggcatg ttcgtccgag taacgctggc
     2821 ggaaaccctc acctggtttc tcgatcgtgc cgggttcgag cttcaattcg cgccgcgtcg
     2881 gcacgaccag cacgtcgagc atgtcaccga tgccggtgcc cgacaggtgg gtgtggctga
     2941 accccatgat cgagccgtct ttctggtgat agcccgagca agcatcccag ccatgggtgt
     3001 tcgtatcggg gctcaattgc gccatcccga acggcagcga ggcgccgggg aaggtgtggc
     3061 cgtgcccgcc ggtgccgatg aagaggttcg gcttgccgat agcgagcgcc tcgcccgcgg
     3121 cgcgcgagca gccggcggg
//
LOCUS       DATMSU010000273         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_71047, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000273 DATMSU010000000
VERSION     DATMSU010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71047"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..615)
                     /locus_tag="VN034_07380"
     CDS             complement(190..615)
                     /locus_tag="VN034_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWV60456.1"
                     /translation="MSMAVGDRDENEPMMDMNTTPLIDVMLVLLIMFIITIPVQTHAV
                     KIDLPVPTDSQSNVDPEKNKVMIDPAGTITWNGTPVDLAQLAQYLEQTKALPVEPELQ
                     VQPDPYARYIVVDNVMAVIKRSGVGKLGFVGNEQYARVF"
     gene            complement(651..1172)
                     /locus_tag="VN034_07385"
     CDS             complement(651..1172)
                     /locus_tag="VN034_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWV60457.1"
                     /translation="MAMSVGDSGGEDAPMSEINTTPLVDIMLVLLIIFLITVPVVLET
                     VNLKLPDVVFEPTTTKPENVLLSIRSADTDGDGEPNADSSACEVYWGQTPVDSRQLLE
                     RGQQKLEKLLEDIGGPQNITEENFPEVHIRGDVNTPYQCIGGVIYTMQYAGFQKIGFI
                     SEPAPGSGTVGRL"
     gene            complement(1297..2112)
                     /locus_tag="VN034_07390"
     CDS             complement(1297..2112)
                     /locus_tag="VN034_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWV60458.1"
                     /translation="MFNLIANAAAAAPAAAHDAGMSLMPASMCVKEEGQNPYGLVPAL
                     CEGGIVSQVTFLVLLIMFIGTLYILFTKLFEQNKVINQGKQVDANFWRAPTLADGASK
                     LEKNSAYRQVVEDGLRANEEHNKLTDPVEAHDWMHGTLERSQNHINSKLNSGLAFLAT
                     VGSTAPFVGLFGTVIGILRALVKIGASGQASIDTVAGPVGEALIMTAIGLIVAVPAVL
                     AFNWLQSRNKSIARRLSTFSNDVLGSIMSNGQVKPATLAPAKAAAPAAAPKKA"
     gene            complement(2168..>2494)
                     /locus_tag="VN034_07395"
     CDS             complement(2168..>2494)
                     /locus_tag="VN034_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWV60459.1"
                     /translation="PAPPPAPAPDLSQAGTPRGNPGRWATNDDYPARAMREEREGTTG
                     FRVTYNVEGRVTSCDVTSSSGHADLDAETCKLIQRRGRFNPGKDRAGNPTGGTYSNRI
                     RWQIPR"
BASE COUNT          455 a          754 c          782 g          503 t
ORIGIN      
        1 agagatcgca gaggaggaag ctcttcccct ctgcgatctc tgcgcctctg cgcgaatcct
       61 atttgaaagc cgcctgtgcg gccatgccaa caatcggtcg aaagcggctt ttgcgcatcg
      121 acaaaagaaa agggccgcgg aattgctccg cgaccccatt cttttttgcc tgacggccct
      181 gatcagaaat cagaagacgc gggcatattg ttcgttgccg acgaagccca gcttgccgac
      241 gccgctgcgc ttgatcaccg ccatgacatt gtcgacgacg atataacgcg cataggggtc
      301 gggctggacc tgcagttcgg gttcgaccgg cagcgccttg gtctgttcca gatattgcgc
      361 cagctgcgca agatcgaccg gcgttccgtt ccaggtgatc gtccccgcgg ggtcgatcat
      421 caccttgttc ttctcggggt cgacattgct ctggctgtcg gtcgggaccg gcaggtcgat
      481 cttcaccgcg tgggtctgga ccgggatggt gatgatgaac atgatgagga gcacgagcat
      541 gacgtcgata agcggcgtcg tgttcatgtc catcatcggt tcgttttcgt cccgatctcc
      601 aactgccatg gacatgcgat tattccttca ttcctaaacg gaaggccgcc ttagaggcga
      661 cccaccgtgc ccgagccagg agccggttcg gaaatgaacc cgatcttctg gaagccggca
      721 tattgcatcg tatagatcac gccgccgatg cactggtacg gcgtgttcac gtcgccgcgg
      781 atatgcactt cggggaagtt ctcttcggtg atgttctgcg gcccgccgat atcttcgagc
      841 agcttttcga gcttttgttg cccgcgttcg agcaactgcc gcgaatcgac cggggtctgg
      901 ccccaataga cttcgcacgc gctgctgtcg gcattgggct cgccatcgcc gtcggtgtcg
      961 gccgaacgga tcgacagcag cacattttcg ggcttcgtcg tcgtcggctc gaagaccacg
     1021 tcgggcagct tgaggttcac cgtctccagc accacgggaa ccgtgatgag gaagatgatg
     1081 agcaacacaa gcatgatgtc cacgagcggg gtcgtgttga tttcggacat cggggcgtct
     1141 tcaccgccac tgtcgcctac actcatcgcc ataggattag catcctgtca caaaattact
     1201 gtcacggact tgggtcgtcg cgccaccggc gccgcgccct cgtccagcgg atcccgccct
     1261 tcgccgcgcg gcgaagggcg gaaatccagg aaccaatcag gccttcttcg gcgccgcggc
     1321 cggagcggcg gccttcgccg gagcgagcgt cgccggcttc acctggccgt tcgacatgat
     1381 cgagccgaga acgtcgttcg agaaggtcga gagacggcgg gcgatcgact tgttgcggct
     1441 ctgcagccag ttgaacgcga gcaccgcggg aaccgccacg atcagaccga tggcggtcat
     1501 gatgagcgct tcaccgaccg ggccggcgac ggtgtcgatc gacgcctgac ccgacgcacc
     1561 gatcttcacg agcgcgcgaa gaataccgat aacggtaccg aacagaccga cgaacggcgc
     1621 ggtcgaaccc acggtcgcga ggaaggcgag gcccgagttg agcttcgagt tgatgtggtt
     1681 ctgcgaacgc tcgagcgtgc cgtgcatcca gtcgtgggct tcgacggggt cggtcagctt
     1741 gttatgctct tcgttggcgc gcagaccgtc ttcgacgacc tgacgatagg cgctgttctt
     1801 ttcgagcttc gacgcgccgt cggcgagcgt cggcgcgcgc cagaaattgg cgtcgacctg
     1861 cttgccctgg ttgatcacct tgttctgttc gaacagcttg gtgaacagga tgtagagcgt
     1921 gccgatgaac atgatcagca ggacgaggaa ggtgacctgc gacacgatgc cgccttcgca
     1981 gagcgcgggg acgaggccgt aggggttctg gccttcttcc ttcacgcaca tcgatgcggg
     2041 catcaggctc atgcctgcgt cgtgagcggc agccggcgcc gcagcggcgg catttgcgat
     2101 cagattaaac atataggaaa ttccctctca aaatattcaa aatcgaaaac aggtggtccg
     2161 gcgccgatca gcgcggaatc tgccaacgga tacggttgct gtaagtgccg cccgtcggat
     2221 tacccgcgcg atccttgccc gggttgaacc ggccgcggcg ctggataagc ttgcaggttt
     2281 cggcatcgag atcggcgtgc ccgctcgacg acgtgacgtc gcaagaggtt acgcgtccct
     2341 cgacattata ggtcacgcgg aaacccgtcg tgccttcgcg ttcttcgcgc atggcgcgcg
     2401 ccggatagtc gtcgttcgtc gcccagcggc ccggattgcc acgcggcgta ccggcctggc
     2461 tgagatcggg cgccggtgcc ggcggcggtg ccgg
//
LOCUS       DATMSU010000274         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_80461, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000274 DATMSU010000000
VERSION     DATMSU010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80461"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VN034_07400"
     CDS             complement(<1..445)
                     /locus_tag="VN034_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60460.1"
                     /translation="MGALDGKVAIITGAGSGIGRASALRFAAEGAKLVIGDKTAAVHE
                     TAKLVKDAGGEVVALEIDAGVEADVASLVAAAKENFGALDVAFANAGIIGDMGGIFDF
                     DPEAWAETLRVNLIGPALMVKHAGKAMVDQGRGGAIVLTASVAGLN"
     gene            complement(445..1221)
                     /locus_tag="VN034_07405"
     CDS             complement(445..1221)
                     /locus_tag="VN034_07405"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWV60461.1"
                     /translation="MSLFDMGGQVALITGSSRGIGKATAEAMAEQGAKVVISSRKADA
                     CEETAAEINARFGEGTAIAVRANISSKEELEILVAETRRTFRKITTLVCNAASNPYYG
                     PGLGISDDQFRKIMDNNILSNHWLISMVAPEMLERGEGSIIIISSIGGLKGSPIIGAY
                     GISKAADMQVARNFAVEFGPRGVRVNCIAPGLIRTDMARALWENEENLKRSTAASTLK
                     RIGEPHEIAGAAVFLASKAGAFTTGQTIVCDGGATISGGA"
     gene            complement(1218..2285)
                     /locus_tag="VN034_07410"
     CDS             complement(1218..2285)
                     /locus_tag="VN034_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWV60462.1"
                     /translation="MTLDAQKAFSGTVAPEGADVLDEAKLTAWMEAHVEGFEGPLTQG
                     KFAGGQSNPTYKISAPSGNYVLRRKPFGPLLPSAHAVDREYKVQAGLYKTGFPVARQY
                     GVCNDDSVVGSWFYVMGMVDGQTIWDGSMPGSSPENRRATYNAMIDTLAALHSVDVEA
                     AGLSDFGKPGNYFGRQVDRWTKQYKLSETETMDEMERLIAWLPATLPEQTRTSVVHGD
                     YRIDNMIFAKDRPEVLAVLDWELSTLGDPLADFTYVAMAWVTENGGRSGVMDLDRKGL
                     GIPELDEVVERYCAATGRDGVPDMNWYFAYNFFRLAGIMQGIKKRVIDGTASSAHAKA
                     MSERVLPLAERAWDFAKKAGA"
     gene            complement(2282..>3181)
                     /locus_tag="VN034_07415"
     CDS             complement(2282..>3181)
                     /locus_tag="VN034_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWV60463.1"
                     /translation="IVEAVGSEVRTVKVGDAVVTCLSAFCGHCEFCVSGRMSLCMGGD
                     TRRPVGSAPRITRPDGSPVNQMLNLSAFSETMLVHEHACVAIDPDMPLDRAAVIGCAV
                     TTGAGTIFNACKVTPGETVAVVGCGGVGLATINAAKIAGAGRIIAADPVPEKRALAVK
                     LGATDVVDALADDAAKQIVELTKGGVDHAIEAVGRPASASLAVASLRRGGTATILGMM
                     PLAEKVGLSAMDLLSGKKLQGAIMGGNRFPVDIPRLVDFYLRGLLDLDSIVAETIPLA
                     KVNEGFDKMRKGDAARSVIVFDQ"
BASE COUNT          516 a         1098 c          996 g          571 t
ORIGIN      
        1 aattcagccc cgcgacgctc gcggtcagca cgatcgcgcc gccgcggccc tgatcgacca
       61 tcgccttccc ggcatgcttc accatcagcg cggggccgat caaattgacg cgtaaagtct
      121 cggcccaggc ttcggggtcg aaatcgaaaa tgccgcccat gtcgccgatg atcccggcgt
      181 tggcgaaagc gacgtcgagc gcgccgaaat tctccttcgc cgccgcaacg agcgaggcga
      241 catcggcctc gacccccgca tcgatttcga gcgcgacgac ctcgccgccg gcatccttca
      301 ccagcttcgc ggtttcatgc accgccgccg tcttgtcgcc gatgacgagc ttcgcaccct
      361 cggcggcgaa gcgcagcgcg ctcgcgcggc cgataccgct gcccgcaccc gtgatgatcg
      421 caaccttgcc gtccaatgcg cccatcatgc gcctcccgaa atcgtggctc cgccatcgca
      481 cacgatggtc tggccggtgg tgaatgctcc cgccttgctg gcgaggaaaa ccgcggcgcc
      541 cgcgatctcg tgcggttcgc cgatgcgctt cagcgtcgag gcggcggtcg atcgtttgag
      601 gttttcttca ttttcccaca aagcgcgcgc catgtcggtg cggatcaggc cgggggcgat
      661 gcagttgacg cgcacgccgc gcggcccgaa ttcgaccgcg aaattgcgcg cgacctgcat
      721 gtcggcggct ttcgaaatgc cgtaggcgcc gatgatcggc gatcctttca gcccgccgat
      781 cgagctgatg atgatgatcg acccttcgcc gcgttcgagc atctcgggcg cgaccatcga
      841 gatcagccag tgattggaca ggatattatt gtccatgatc ttgcggaact ggtcgtcgct
      901 gatcccgagc ccggggccat aatagggatt cgatgcggcg ttgcagacga gcgtggtgat
      961 cttgcggaag gtgcggcgcg tctcggcaac gagtatctcc agctcttctt tcgacgaaat
     1021 gttcgcgcgc actgcaatcg cggtgccttc gccgaagcgc gcgttgatct cggccgcggt
     1081 ttcctcgcac gcgtcggcct tgcggctcga aatcacgacc ttggcgccct gctcggccat
     1141 cgcttccgcc gtggccttac cgatgccgcg cgacgatccg gtgatcagcg cgacctggcc
     1201 gcccatgtcg aacaggctca tgcgccggcc tttttcgcga aatcccacgc acgctcggcg
     1261 agcggcagga cgcgctcgga catcgccttg gcatgggcgg aagacgcggt gccgtcgatc
     1321 acgcgcttct tgatcccctg catgatgcct gcgaggcgga agaaattata ggcgaaatac
     1381 cagttcatgt cgggcacgcc gtcgcggccc gtcgccgcgc aatagcgttc gaccacctcg
     1441 tcgagttcgg ggatgccgag gcccttgcgg tcgagatcca tcacccccga gcgcccgcca
     1501 ttctcggtca cccacgccat cgcgacatag gtgaaatcag cgagcgggtc gcccagcgtc
     1561 gacagttccc agtcgagcac cgcgagcact tcgggacgat ccttggcgaa gatcatattg
     1621 tcgatgcggt aatcgccgtg gaccacgctg gtgcgtgtct gttcgggcag ggtcgcgggc
     1681 agccacgcga tcagccgctc catctcgtcc atcgtctcgg tctcggacag cttgtactgc
     1741 ttcgtccagc gatcgacctg gcggccgaaa taattgccgg gcttgccgaa atccgaaagc
     1801 cccgccgcct cgacatcgac gctgtgcagc gcggcgagcg tgtcgatcat cgcattgtag
     1861 gtcgcgcggc ggttctcggg gctcgacccg ggcatcgacc cgtcccaaat cgtctggccg
     1921 tcgaccattc ccatcacata gaaccagctg ccgaccacgc tatcgtcgtt gcagacgccg
     1981 tactgacgcg cgacggggaa ccccgtctta tagagccccg cctgcacctt atattcgcgg
     2041 tcgaccgcgt gcgccgaagg cagcagcggg ccgaagggct tgcgccgcag cacataattg
     2101 cccgacggcg ccgaaatctt gtacgtcggg ttcgactggc cgccggcgaa tttgccctgc
     2161 gtcagcgggc cttcgaagcc ttcgacatgg gcctccatcc acgccgtcag cttcgcctcg
     2221 tcgagcacat cggcgccctc gggcgcgacg gtgccgctga acgccttttg cgcgtcgagc
     2281 gtcattggtc gaacacgatc accgaccgtg cggcgtcgcc ctttcgcatc ttgtcgaacc
     2341 cttcgttgac cttggcgagc gggatcgttt cggcgacgat cgagtcgagg tcgagcagcc
     2401 cgcgcagata gaaatcgacg aggcgcggga tgtcgaccgg gaaacggttg ccgcccatga
     2461 tcgcgccctg caatttcttg cccgaaagca ggtccatcgc gctgagcccg accttttcgg
     2521 cgagcggcat catgccgagg atcgtcgcgg tgccgccgcg gcgcagcgat gcgaccgcga
     2581 ggctggccga ggccggacgt ccgaccgcct cgatcgcatg gtcgacgccg cccttggtca
     2641 gctcgacgat ctgcttcgcg gcatcgtcgg cgagtgcatc gacgacgtcg gtcgcgccga
     2701 gcttcaccgc cagcgcacgc ttttcgggaa ccggatcggc ggcgatgatg cgccccgcgc
     2761 cggcgatctt cgcggcgttg atcgtggcca agccgacccc gccgcaaccg acgactgcga
     2821 cggtctcgcc cggtgtcacc ttgcacgcgt tgaagatcgt ccccgcgccc gtcgtcaccg
     2881 cgcagccgat caccgcggcg cggtcgagcg gcatgtcggg gtcgatcgcg acgcacgcat
     2941 gttcgtggac gagcatcgtt tccgaaaagg ccgagaggtt gagcatctgg ttcaccggcg
     3001 acccgtcggg gcgcgtgatg cgcggtgccg atccgaccgg ccgccgcgtg tcgccgccca
     3061 tgcacagcga catgcggccc gacacacaga attcgcagtg gccgcagaag gcgctgaggc
     3121 aggtgacgac cgcgtcgccg accttcaccg tgcgcacttc gctgccgacc gcctcgacga
     3181 t
//
LOCUS       DATMSU010000275         6373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_52182, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000275 DATMSU010000000
VERSION     DATMSU010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6373
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52182"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            359..1204
                     /locus_tag="VN034_07420"
     CDS             359..1204
                     /locus_tag="VN034_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWV60464.1"
                     /translation="MRFEGTSAYIATDDLKVAVNAATLLRRPLLVKGEPGTGKTVLAE
                     EIAKAFDAPLITWNIKSTTKAQQGLYEYDAVARLRDGQLGEERVHDIRNYIRKGKLWE
                     AFTSPKLPVLLIDEIDKADIEFPNDLLQELDRMAFHVYETDETVTAKERPIVVITSNN
                     EKELPDAFLRRCFFHYIKFPDRDTMAEIVEVHFPGIQKTLVSRAMDIFYEVREVPGLK
                     KKPSTSELIDWLKLLLNEDMPLEVLQNRDVGKAIPPLHGALLKNEQDVMLFEKLAFMA
                     RRQGS"
     gene            complement(1291..2688)
                     /locus_tag="VN034_07425"
     CDS             complement(1291..2688)
                     /locus_tag="VN034_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWV60465.1"
                     /translation="MKYDPINPAEPILDQSVASDCGELAVGCSDAAGRIKIATDQMDR
                     QIGELGRLEEYVVSLEADQRQIADSTDEAKLLSARACEQLDTGAERVNAAVSEFRSVI
                     DLIARLGAHVTNFAAVMEQVQQVSQSIEAIAKTTNMLALNAAIEAERAGDAGRTFAVV
                     AAEVKKLAQNTRGATDEIRRSIGSLATEASGLVAEIQSGVEQSSRAEAQFETITDALH
                     DATHLVALLDDQSDRIAQSSAMVHANGAKVREALDLVVTSVRDNSSTLDGTRTSILTM
                     EHVSNRMFNAVISAGVSPQDSAIVALAAKVRDEFVGLAEKAIARGELAMEQLFDTNYV
                     RVPNSNPERFRTSLCDWADANWRPLFDRIVAQHPEIKMSSAGDMNGFLPTHITDCSRA
                     PTGDLEHDTAHCRNGRLLYDETDAAAKRSTAPFFMTVYRQEGDGINYLTVRNVYMPAV
                     IGGRRWGDVEVAYQL"
     gene            2818..3996
                     /locus_tag="VN034_07430"
     CDS             2818..3996
                     /locus_tag="VN034_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWV60466.1"
                     /translation="MFFGFLDELRAAGIPASLKEHLMLLEALDREVIDRSPEQFYYLS
                     RAIYVKDEGQLDRFDQVFNKVFKGIISDYGQNPVDVPADWLKAVAEKFLTPEEMAAIE
                     SLGDWDKIMETLKERLEEQQKRHEGGGKWIGTGGTSPFGNSGYNPEGVRIGGESKHKR
                     ALKVWEKREFQNLDNTKELGTRNIKIALRRLRKFAREGAADELDIPGTIDGTARQGWL
                     DIKMRPERRNAVKLLLFLDVGGSMDPFIKLCEELFSAATTEFKNLEFFYFHNCPYEGV
                     WKDNKRRWSERHQMWDILHKYGHDYKVIFVGDASMSAYEITHPGGSVEHFNEESGAVW
                     LQRITHVYPAAVWLNPVPEAHWGYTQSVKLIKQLMNDRMYPLTLAGLDDAMRELTRKH
                     "
     gene            4003..4695
                     /locus_tag="VN034_07435"
     CDS             4003..4695
                     /locus_tag="VN034_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HWV60467.1"
                     /translation="MGAFRDRLRGWFGRYEIEDPRPIAADAPYTFYLPSENELLAVSP
                     GDLVKLAFRSIPDSSEWGAERMWVIVTAAEGARLTGTLDNHPLDMPQLKAGDRVTFTR
                     GHVMDIDWSADRAAPPPTAPARREYWDRCFVDDQILDGWLKIEYLYREEPDPPDEGDA
                     YPDSGWRFRCDTRGLTNEEYDNPSFSYIAVGKVLNEDDSWLHLIDAPVGSAFLRNWET
                     GEFVPTEMDGSE"
     gene            4737..5543
                     /locus_tag="VN034_07440"
     CDS             4737..5543
                     /locus_tag="VN034_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase Amj family protein"
                     /protein_id="HWV60468.1"
                     /translation="MFDLPLVTILLLTGFINLIGALAYAARIAGVRTRRIAMSFALFN
                     ILVLFSRTSNSFLGPFLAKRIETRIHDGSGASLFIDMQLVLAAASVATLLGILLVPTG
                     QRMFAAAIGWYQNNRSTTKLALKAVSPGGIRTLGRSLTVPSLSHLKDWRMPKGIGWGV
                     LIANCLAQSLLAVGVVASLYAGYLAPEFRVTASQLSALINGFATILLFAFIDPQLSVM
                     TDDAVEGKVDEADFRRAITLISLSRLAGTILAQALLLPAAMLIAWVAVYV"
     gene            5536..>6373
                     /locus_tag="VN034_07445"
     CDS             5536..>6373
                     /locus_tag="VN034_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HWV60469.1"
                     /translation="MSEPERGHSRFHAVRSRLEAMAVEPGPRGWCLEFLVFGFKQGWA
                     CLFGGLMLALLLGTHLFWPEDAPLHRYDAITIGAVLIQLAMLAFRLETPKEALVILIF
                     HIVGTVMELFKTAAGSWQYPEASLLHIGAVPLFSGFMYAAVGSYIARVWRIFDFRYSG
                     YPPAWASYALAAAIYVNFFAHHWVYDIRWFLFAATALLFWRCQVWFRPLHVHRRMPLL
                     VGWGLVALFIWFAENIGTFARAWTYPSQNDGWHMVGVEKLGSWYLLMIISFVLVSLVQ
                     RPK"
BASE COUNT         1141 a         2050 c         1970 g         1212 t
ORIGIN      
        1 ctgcaccctg cccgacctgc cgggcaccgc gcggctcgcg acagcggtcg ccgacacgcc
       61 cgcgatcgag cgcgcgatgg tgttcggtgc gcagcagctt atcacgcagc acagcggcct
      121 ctgggatttg atggccgcac gcgcggcgag ccgcgcgcac ccgtaaaata tcaggcaacc
      181 gagcacgaca ggttcccctc ccgcttgcgg gaggggctag gggaaggcat gtctgatggc
      241 acttccgatg aaacaggccc acccccgacc cctcccgcaa gcgggagggg agacaagctt
      301 gcaccgccct cccacccccg ctatgcccgc ttgacgttca cgttaaggga aagagccgat
      361 gcgcttcgaa ggcaccagcg cctatatcgc cactgacgac ctcaaggtcg cggtcaacgc
      421 cgcgacgctg ctgcgccgtc cgctgctggt gaagggcgaa cccggcaccg gcaagaccgt
      481 gctcgccgag gaaatcgcca aggctttcga cgcgccgctg atcacctgga acatcaagtc
      541 gacgacgaag gcgcagcagg gcctctacga atatgacgcg gtggcgcgcc tgcgcgacgg
      601 acagctcggc gaagagcgcg tccacgacat ccgcaactat atccgcaagg gcaaattgtg
      661 ggaggcgttc acctcgccca agctccccgt cctgctgatc gacgagatcg acaaggccga
      721 tatcgaattc ccgaacgatt tgcttcagga actcgatcgc atggcgttcc atgtctatga
      781 gaccgacgag acggtgaccg cgaaggaacg cccgatcgtc gtcatcactt cgaacaatga
      841 aaaggaactg cccgacgcct tcctgcgccg ctgtttcttc cactatatca aattccccga
      901 ccgcgacacg atggccgaga tcgtcgaggt ccacttcccg ggcatccaga agacgctggt
      961 cagccgcgcg atggatatct tctacgaagt gcgcgaggtt ccgggcctca agaaaaaacc
     1021 ctcgacgagc gagctgatcg actggctcaa gctgctgctc aacgaggata tgccgctcga
     1081 ggtgctccag aaccgcgacg tcggcaaggc gatcccgccg ctgcacggcg cgctgctcaa
     1141 aaatgagcag gatgtgatgc tgtttgagaa gctagcgttt atggcgcggc ggcagggtag
     1201 ctgaatatta tcgtcgtccc agcgaaagct gggaccgcta tcgacgaagc gttcccggtt
     1261 gagcgatccc agctttcgct gggatgacgg ctataattga tacgcgacct cgacgtcgcc
     1321 ccagcggcgc ccgccgatca ctgcgggcat atagacgttg cgcacggtca gataattgat
     1381 gccgtcgcct tcctgccgat agaccgtcat gaagaacggc gcggtgctgc gcttcgccgc
     1441 ggcgtcggtt tcgtcataga gcagccggcc gttgcggcaa tgcgcggtgt catgttcgag
     1501 gtcgccggtc ggcgcgcgcg agcagtcggt aatgtgcgtc ggcagaaagc cgttcatgtc
     1561 gcccgccgag gacatcttga tctcgggatg ctgcgcgacg atgcggtcga acagcgggcg
     1621 ccaattggcg tcggcccagt cgcagaggct agtgcggaaa cgttcggggt tcgagttcgg
     1681 cacgcgaaca taattggtat cgaaaagctg ctccatcgcc agctcgccgc gcgcgatcgc
     1741 cttttcggca aggccgacga actcgtcgcg cacctttgcc gcgagggcga cgatcgccga
     1801 atcctgcggg ctgacccccg ccgagatcac cgcgttgaac atgcggttcg acacatgctc
     1861 catcgtcagg atcgatgtgc gcgtgccgtc gagcgtcgaa ctattgtcgc gaaccgacgt
     1921 caccaccagg tcgagcgcct cgcgcacctt cgcgccattg gcatgcacca tcgcgctcga
     1981 ctgcgcgatc cggtcgctct ggtcgtcgag tagcgcgacg agatgcgtcg catcgtggag
     2041 cgcgtcggtg atcgtctcga actgcgcttc ggcgcggctc gactgttcga cgcccgactg
     2101 gatttcggcg acgagcccgc tcgcctcggt ggcaaggctg ccgatcgaac ggcggatctc
     2161 gtcggtcgcg ccgcgcgtgt tctgcgcgag tttcttcacc tcggccgcga cgaccgcgaa
     2221 agtgcgcccc gcatcgccgg cgcgctcggc ctcgatcgcg gcgttcaatg cgagcatgtt
     2281 ggtggtcttg gcgatcgcct cgatcgactg gctgacctgc tgaacctgct ccatcaccgc
     2341 ggcgaaattg gtgacatgcg cgccgagccg cgcgatgagg tcgatgaccg aacggaattc
     2401 gctgaccgcc gcgttgacgc gctcggcgcc ggtatcgagc tgttcgcacg cgcgcgccga
     2461 cagcaatttc gcctcgtcgg tcgaatcggc gatctggcgc tgatcggcct cgaggctgac
     2521 gacatattcc tcgagccggc cgagttcgcc gatctgccgg tccatctggt cggtcgcaat
     2581 cttgatccgc cccgcagcat cgctgcaccc cacggccagc tcgccgcagt cgctcgccac
     2641 cgactggtcg aggatcggct ccgccggatt gatcggatca tatttcattg gcttgcccac
     2701 gtccctgaat gatcgagaag ctatgccgga gattggttaa cggggcgtca atccggccgc
     2761 actttccgta aacgtaaagt gattgcaccc gctgccgcga caggatagtt cttctccatg
     2821 tttttcggct ttctcgacga gcttcgcgcc gcgggcattc ccgcgagcct caaggagcat
     2881 ctgatgctgc tggaggcgct cgaccgcgag gtgatcgacc gcagccccga gcaattctat
     2941 tatctcagcc gcgcgatcta tgtgaaggac gagggccagc tcgaccgttt cgaccaggtg
     3001 ttcaacaagg tcttcaaggg catcatttcc gattacggcc agaacccggt cgacgtcccc
     3061 gccgactggc tgaaagcggt cgccgaaaaa ttcctgacgc ccgaggaaat ggcggcgatc
     3121 gaatcgctcg gcgactggga caagatcatg gagacgctga aggagcggct cgaggaacaa
     3181 cagaagcgcc acgagggcgg cggcaagtgg atcggcaccg gcggcacctc gcccttcggc
     3241 aattcgggct ataatcccga gggcgtgcgg atcggcggcg agagcaagca caagcgcgcg
     3301 ctgaaggtct gggaaaagcg cgagttccag aacctcgaca acaccaagga gctcggcacc
     3361 cgcaacatca agatcgcgct ccgccgcctg cgcaagttcg cgcgcgaggg cgcggccgac
     3421 gagctcgata ttccgggcac gatcgacggc accgcgcggc agggctggct cgacatcaag
     3481 atgcgtcccg aacggcgcaa tgcggtcaag ctgctgctct tcctcgacgt cggcgggtcg
     3541 atggatccgt tcatcaagct gtgcgaagaa ctgttcagcg ccgcgacgac cgaattcaag
     3601 aatctcgaat tcttctattt ccacaattgc ccgtacgagg gcgtgtggaa ggataataag
     3661 cggcgctggt ccgaacggca ccagatgtgg gacatcctcc acaaatatgg ccatgattat
     3721 aaggtgatct tcgtcggcga cgcgtcgatg tcggcctatg aaatcaccca tccgggcggc
     3781 agcgtcgagc atttcaacga ggaatcgggc gcggtgtggc tccagcgcat cacccatgtc
     3841 tatccagcgg cggtgtggct gaacccggtg cccgaagcgc attggggcta cacccagtcg
     3901 gtgaagctga tcaaacagct gatgaacgac cgcatgtatc cgctcaccct cgccgggctc
     3961 gacgatgcga tgcgcgagct gacgcgaaag cattgacgcg gcgtgggcgc gttcagggac
     4021 aggctgcgcg gctggttcgg acggtatgag atagaagatc cgcggccgat cgcagcggac
     4081 gcgccttata ctttctatct tccttcagag aatgagctgt tggcggtatc gcccggcgac
     4141 ctcgtcaaac tggctttccg gtcgattccc gatagcagcg aatggggcgc agaacgcatg
     4201 tgggtcatcg tgacggcggc agagggagca cggttgaccg gtacgctcga caaccatcca
     4261 ctcgacatgc cccagctcaa ggccggtgat cgcgtgactt ttacccgtgg gcacgtcatg
     4321 gacatcgact ggagcgcgga tcgcgccgcc cctcctccga ccgcgcctgc ccggcgggaa
     4381 tattgggatc gctgcttcgt cgatgaccaa attctcgatg gttggctcaa gatcgaatat
     4441 ctttatcgcg aggaacccga cccgcctgac gagggcgacg cctatcccga cagcggctgg
     4501 cgttttcgtt gcgacacgcg cggcctgacc aacgaagaat atgataatcc gagtttcagc
     4561 tacatcgctg tcggcaaggt gttgaacgag gacgatagct ggctgcacct gatcgacgcc
     4621 ccggtgggca gcgcctttct ccgcaattgg gaaaccggcg aatttgtccc gaccgaaatg
     4681 gacggctctg agtgaaaccg ctcgaaatcg ctttgccctc cggctaatcc ccgacgatgt
     4741 tcgacctccc cctcgtcacc attctgctcc tcaccggctt catcaacctg atcggcgcgc
     4801 tggcttatgc cgcgcgcatc gccggagtgc ggacgcggcg gattgcgatg tctttcgcgc
     4861 tgttcaacat tctcgtgctc ttttcgcgca cctcgaacag cttcctcggc ccctttctcg
     4921 ccaagcggat cgagacgcgc atccacgacg gcagcggcgc gtcgctgttc atcgacatgc
     4981 agctcgtgct cgccgcggcg agcgtcgcga cgctgttggg gatcctgctc gttccgaccg
     5041 gccagcgcat gttcgcggcc gcgatcggct ggtatcagaa caatcgctcg acgacgaagc
     5101 tcgcgctgaa ggccgtaagc cccggcggca tcaggacatt gggccgatcg ctgaccgtcc
     5161 ccagcctttc gcatctcaag gactggcgga tgccgaaggg gatcggttgg ggcgtgctga
     5221 tcgccaactg cctcgcgcaa tccctgttgg ccgtcggcgt cgtcgcgtcg ctctacgccg
     5281 gctatctcgc gcccgaattc cgcgtcaccg cgtcgcagct ttcggcgctg atcaacggtt
     5341 tcgcgacgat cctgctgttc gccttcatcg acccgcaact gtcggtgatg accgacgacg
     5401 cggtcgaggg aaaagtcgac gaggccgatt tccgccgcgc gatcacgctg atctcgctga
     5461 gccgcctcgc gggcacgatc ctcgcgcagg cgctgttgct gcccgccgcg atgctgatcg
     5521 cgtgggtcgc cgtctatgtc tgaaccggaa cgcggccata gccgcttcca cgccgtccgg
     5581 tcgcggctcg aagcgatggc ggtcgagccg ggaccgcgcg gctggtgcct cgaatttctc
     5641 gtcttcggct tcaagcaggg ctgggcctgc ctgttcggcg gcctgatgct cgcgctgctg
     5701 ctcggcacgc atctgttctg gcccgaggat gcgccgctcc atcgctacga cgcgatcacg
     5761 atcggtgcgg tgctgatcca gctcgccatg ctcgccttcc ggctcgagac gccgaaggaa
     5821 gcgctcgtca tcctgatctt ccacatcgtc ggcacggtga tggagttgtt caagaccgcc
     5881 gcgggttcgt ggcaatatcc cgaagcgagc ctgctccaca tcggcgccgt tccgctcttt
     5941 tcgggcttca tgtatgccgc ggtcggcagc tatatcgccc gcgtctggcg catcttcgac
     6001 tttcgctatt cgggctatcc gcctgcgtgg gcgagctacg cgctggcggc ggcgatctac
     6061 gtcaatttct tcgcgcacca ttgggtgtac gacatccgct ggtttctctt cgccgcgacc
     6121 gcgctgctct tctggcgctg tcaggtgtgg ttccgcccgc tgcacgtcca tcgccgcatg
     6181 cccttgctcg tcggttgggg tcttgtcgcg ctgttcatct ggtttgccga aaatatcggc
     6241 accttcgcgc gcgcatggac ctatccgagc cagaacgacg gctggcatat ggtcggcgtc
     6301 gaaaagctcg gcagctggta tctgctgatg atcatttcgt tcgtgctggt cagcctggtc
     6361 cagcggccga agg
//
LOCUS       DATMSU010000276         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_23620, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000276 DATMSU010000000
VERSION     DATMSU010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23620"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..739
                     /gene="ubiG"
                     /locus_tag="VN034_07450"
     CDS             32..739
                     /gene="ubiG"
                     /locus_tag="VN034_07450"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HWV60470.1"
                     /translation="MAREAAHFGEMAADWWNPRGSSAMLHRLNPVRLAFVRAAIDSHW
                     DGDVAGFTPLAGKTVLDVGCGAGLLAEPLARMGAKVTGVDAAPENIAAAQGHAAGQGL
                     AITYFAGELAALPRATFDLVTSMEVVEHVADPAAFIGELAGRLAPGGLMILSTPNRTM
                     LSKLLLVEAAERVGAVPRGTHDWDQFLKPEELTNLLEGAGLEVIDRTGLSPSAAKGFK
                     LGGSEALNYLVAARWPG"
     gene            727..1329
                     /locus_tag="VN034_07455"
     CDS             727..1329
                     /locus_tag="VN034_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWV60471.1"
                     /translation="MAGLSRDPRVDAYIAKRQPFAQPILNHLRELVHKHAPGTEETLK
                     WGVPAFVLNGQNLAGMAAFKEHATFGFWRDEEVTGSPRDTGAMGSMGRLATLADLPND
                     KQMADYIRKAVALCADGKPKRPAPKPKPTLDLPDDLGAALKANAAAQGHWDAFSPGKR
                     RDYIEWVLEAKREETRVKRIETIVAQVAEGKDRNWKYKAC"
     gene            complement(1780..2151)
                     /locus_tag="VN034_07460"
     CDS             complement(1780..2151)
                     /locus_tag="VN034_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60472.1"
                     /translation="MTNQWRRHVMHKIEQGVGSVVAADTRTTMASVDAAYLAQARMAA
                     SIVEAATASDLPAGATQELLEAVTDSMTQVVAGRTKLIKALGQLQQIQSKSNLREVGF
                     GCPGGLPSQASLATQSEQTTS"
     gene            complement(2170..2649)
                     /locus_tag="VN034_07465"
     CDS             complement(2170..2649)
                     /locus_tag="VN034_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV60473.1"
                     /translation="MKQNFPLPPEAAALAARLSERQRECLILVRNGLTSKEIGRLLSL
                     SPSTVDNHLNAATERLGCSSRAIAAQILEMAEQFAGQPLADHERKFNKNSSAYSGGTT
                     APPTSRRSYFLPPLGGSPNEETTLRRITHIGLIAIAATMAFAAITITVAGIIELFSK"
     gene            complement(2932..3603)
                     /locus_tag="VN034_07470"
     CDS             complement(2932..3603)
                     /locus_tag="VN034_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV60474.1"
                     /translation="MWQLYQFPLCPFSRKVRLLLGEKGVGYELVRESPWERRDEFIDL
                     NPAGRTPVMVDQTRGQVLIDSMAIAEYFEETVEGKAMINGTAANRAEIRRLTAWFDHD
                     FYYEVTGPLLFERMQKRIVHRQPPDGGALREAMKAANNHLDYVDYLIDHRTWLAGATM
                     SLADLAAAAHISVADYLGGIDWTGHEQTKGWYSGLKSRPSFRPLLAERMEIVSPPKYY
                     EDVDF"
     gene            complement(3764..4198)
                     /locus_tag="VN034_07475"
     CDS             complement(3764..4198)
                     /locus_tag="VN034_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60475.1"
                     /translation="MRSTAKLALAALPLLALAAPLPVAANEMQGAVATLNDPVAQDRM
                     AETITAMVGALMQMQVGPLARAVAEIDPESDAAYIPPDATLGEVTGRDPYYAERMGDD
                     VRATTRMAGHAASALAAYAPVLRDMARDLAAQWEYERDAARR"
BASE COUNT          823 a         1263 c         1382 g          788 t
ORIGIN      
        1 gcaaaatccc gcgcccgcgc aatcaagcgt cgtggcgcgc gaagccgccc attttggcga
       61 gatggcggcc gattggtgga accccagggg cagctcggcg atgctccacc ggctgaaccc
      121 ggtgcggctg gcctttgtcc gggcggcgat cgattcgcac tgggacggcg atgtggcggg
      181 cttcaccccg ctggcgggca agaccgtcct cgacgtcggt tgcggcgcgg ggctgctcgc
      241 cgaaccgctc gcgcggatgg gcgcgaaggt caccggcgtc gatgcggcgc ctgagaatat
      301 cgccgccgcg cagggtcatg cggcggggca ggggcttgcg atcacctatt tcgcgggcga
      361 actcgccgcg ctgccgcgcg cgaccttcga cctcgtcacc tcgatggaag tcgtcgagca
      421 tgtcgccgac cccgcggcct tcatcggcga actcgcaggc cggctcgcgc ccggcgggct
      481 gatgatcctg tcgaccccga accggacgat gctgtcgaag ctgctgctcg tcgaggcggc
      541 cgagcgcgtc ggcgcggtgc cgcgcgggac gcacgactgg gaccagtttc tgaagcccga
      601 ggaactgacg aacctgctcg aaggcgcggg gctcgaggtg atcgaccgca ccggcttgtc
      661 gccctcggcg gcgaagggct tcaagctcgg cggcagcgag gcgctcaact atctggtcgc
      721 ggcgcggtgg ccgggctgag ccgcgacccg cgcgtcgacg cctatatcgc gaagcggcag
      781 cccttcgcgc agccgatcct caaccatttg cgcgaactgg ttcacaaaca tgcgccgggc
      841 accgaagaga cgctgaaatg gggcgtaccg gccttcgtgc tgaacggcca gaatctcgcg
      901 ggcatggcgg cgttcaagga acatgcgacc ttcggtttct ggcgcgacga ggaagtgaca
      961 ggctcgccgc gcgacaccgg cgcgatgggc agcatgggac ggctcgcgac gctcgccgac
     1021 ctacccaacg acaagcagat ggcggattac atcaggaagg cggtcgcact ctgcgccgac
     1081 ggcaagccca agcgcccggc gccgaaaccc aagccgacgc tcgacctgcc cgatgacctc
     1141 ggcgcggcgc tcaaagccaa tgccgcggcg caaggccatt gggacgcctt ctcgcccggc
     1201 aaacggcgcg actatatcga atgggtgctc gaggcgaagc gcgaggaaac gcgcgtcaaa
     1261 cgcatcgaaa cgatcgtcgc gcaggtcgcc gagggcaagg accgcaactg gaagtacaag
     1321 gcctgctagt taatcgacca cgctagtgaa tgcgggggaa ggcttcgagc gccgttgtcc
     1381 gtcgcgataa gttccatagg ccatgcaggc gagcgctggc agcgaccaga aggcactggc
     1441 cgcgaaaacg acgaacgggt tgaggttgcg gaaaaccaat atgctcaccg acgccgctat
     1501 accgaccacc agaaagcccg caaaccagac gggccaaaag cgatttcccg tcagtgcaag
     1561 gtagagcgag tatataagaa tcgaagagtc gatcggaatg atataataaa gataagatgg
     1621 aaattgaaaa cttacctgta tggtatgtgt tgctattgaa gacatcagta agtataaaca
     1681 gaatctacgc tcgacatttt caccgaatat cgatgatatg gcgagcgtag acagaaggta
     1741 gaaatagaat aatatttgga tcatttaatc ctgttgaatc taggaggtgg tctgttcgct
     1801 ctgcgtcgcc aaggacgctt ggcttgggag gccgccaggg cagccgaagc cgacttcccg
     1861 caaattgctc ttgctctgta tctgctgaag ctggccaaga gccttaatca gtttggttcg
     1921 acccgccacg acttgtgtca tgctgtcggt caccgcttcc aacaattctt gcgtagcgcc
     1981 tgccggcaga tcggaggccg tggccgcttc gacgatgctg gccgccattc gggcttgggc
     2041 gagataggcg gcatctaccg acgccatcgt tgtccgcgta tccgccgcaa ccacgcttcc
     2101 gaccccttgc tctatcttgt gcatcacatg cctcctccat tgattggtca tggatgcttc
     2161 cgaaaaaggc tacttgctga acagctcaat tatgcccgca acggttattg tgatggcggc
     2221 gaacgccatt gtggcggcaa ttgctatcaa accgatatgc gtaatccggc gtagcgtcgt
     2281 ttcctcattc ggcgagcctc caagcggtgg cagaaaatag ctgcggcgcg atgttggagg
     2341 ggcagtcgtt ccacctgaat atgccgagct atttttgtta aactttcttt cgtggtcagc
     2401 caaaggttga ccggcaaatt gctctgccat ctccaggatt tgggccgcga tggcgcgcga
     2461 tgagcagcca agtctttcgg tcgctgcgtt gagatgattg tcgaccgtcg acggagagag
     2521 cgacagcaaa cgaccgatct ccttggaagt aaggccgttt ctgacaagaa tcaggcattc
     2581 acgttgacgt tcgctaaggc gcgcggccag agcggcggct tcgggcggca agggaaaatt
     2641 ctgcttcact ttggattcaa ttgctgagtc gtggggcgat gaccgagcct agtagctatg
     2701 gttaacatcg cctaggcaga ttggccggtt aattggtgcc aatggtgtaa cgatctttgt
     2761 tgcggcagtg cggtgcgtag aacgacacgg cggatttgcc atagctccgg agggtcattg
     2821 ggtgcggggg cgtgccaatg gctgcttact gtccagtcgg gtggcgccga ctgcacccgc
     2881 cattacccgg tcgtttagga tgtgggttag agaaaggggg aagctgggga tttaaaaatc
     2941 cacatcctca taatatttcg gcgggctcac gatttccatc cgctcggcca gcagcgggcg
     3001 aaagctcggg cgcgacttca ggcccgaata ccagcccttg gtctgctcgt gcccggtcca
     3061 gtcgatcccg ccgagatagt cggcgaccga gatatgcgcg gcggcggcca ggtcggcgag
     3121 gctcatcgtc gcgccggcga gccaggtgcg gtgatcgatc agatagtcga catagtcgag
     3181 atggttgttc gccgccttca tcgcctcgcg cagcgcgccg ccgtcgggcg gctggcggtg
     3241 gacgatgcgc ttctgcatcc gctcgaacag caagggcccg gtgacttcat aatagaaatc
     3301 atggtcgaac catgcggtca gccggcggat ctcggcgcgg ttggccgcgg tgccgttgat
     3361 catcgccttg ccttcgacgg tttcctcgaa atattcggcg atcgccatgc tgtcgatcag
     3421 cacctgcccg cgcgtctgat cgaccatcac cggggtacgc cccgccggat tgaggtcgat
     3481 gaactcgtcg cggcgctccc acggcgattc gcgcaccaat tcgtaaccca cgcccttttc
     3541 gcccagcaaa aggcggacct tgcgcgaaaa gggacagagc gggaattgat agagttgcca
     3601 cataggcttg gcatagcggc agcggggcgc cgcgcgcaac ggctatggga attacgtcgc
     3661 cagtttactc gtcatcccgg cgaaggccgg gatctcgacc tatcggtatg aagcgagggt
     3721 gagatcccgg tcttcgccgg gatgacgaag aaagacgagg agcttagcgc cgcgcggcgt
     3781 cgcgctcata ttcccactgg gcggcaagat cgcgcgccat gtccctgagg acgggggcat
     3841 aagccgcgag cgccgacgcg gcatggcccg ccatgcgcgt cgtcgcgcgg acatcgtcgc
     3901 ccatgcgctc ggcataatag ggatcgcgcc ccgtcacttc gcccagcgtc gcgtcgggcg
     3961 ggatataggc ggcgtcggac tcgggatcga tttcggccac cgcccgcgcg agtgggccga
     4021 cctgcatctg catcagcgcg ccgaccatcg cggtgatcgt ttcggccatc cggtcctgcg
     4081 cgaccggatc gttgagcgtc gccacggcgc cctgcatctc attggcggcc acaggcagcg
     4141 gcgcggcgag cgcgagcagg gggagggcgg cgagggccag ttttgcggtc gagcgcatga
     4201 tttcatcctt tcgggatgcg gaaatcgatg cggggctata gccgggcgac gctttc
//
LOCUS       DATMSU010000277         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_184858,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000277 DATMSU010000000
VERSION     DATMSU010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184858"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VN034_07480"
     CDS             complement(<1..189)
                     /locus_tag="VN034_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWV60476.1"
                     /translation="MSFNKDRRGHRGRGKRDDFGNFDSFEPAPYGGDSYGGGRGGFGD
                     RDRGGFGGGDRGGGFGGGD"
     gene            450..1067
                     /locus_tag="VN034_07485"
     CDS             450..1067
                     /locus_tag="VN034_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWV60477.1"
                     /translation="MTVYFHEEDLPEGVLRPGPVAIDTETMGLNPLRDRLCLVQISDG
                     SGDEHLVRFKPGSAYDAPVLKAILTDPERLKLFHFARFDIAALQQALGVVTAPVYCTK
                     IASKLVRTYTDRHGLKELVRELLGQEVSKQQQSSDWGAAELSDAQRDYAASDVRFLHA
                     MKAILDARLAREGRTALAQACFDFLPTRAALDLAGWPEVDIFAHS"
     gene            1088..1726
                     /gene="lptC"
                     /locus_tag="VN034_07490"
     CDS             1088..1726
                     /gene="lptC"
                     /locus_tag="VN034_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWV60478.1"
                     /translation="MSERADLDRTMRRMWAASGSSHDRVVRILRFGLPLVIGVVAAVL
                     VFSPFTQRAEISFLLAKDKVDMASERMKVTRAEYRGQDAKGQPFALLAGSAVQKSSAE
                     PIVRMTDLSGAIKLADGPATIVAKSGAYNMETEKVAVPGTVQVKSANGYSIDASNVAV
                     DLNTRTLSGEGGVEGTLNIGRFSANRLTADLDKRIVRLEGNARLRINQGVLK"
     gene            1723..>2192
                     /locus_tag="VN034_07495"
     CDS             1723..>2192
                     /locus_tag="VN034_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HWV60479.1"
                     /translation="MNRLWTLRSMALAGAGFALTSLAFLSAGGGDAAQAQALANHNSN
                     APVDFAAGSIEVQDRADRVVLAGNVRVTQAGLTVTAPRMTVAYTRAGGTDVNRLDATG
                     GVTVVKGDEMAKGNVAIYDLDRRLITMVGNVELRQRGNNLRGGRLVIDLNSGRAT"
BASE COUNT          396 a          722 c          736 g          338 t
ORIGIN      
        1 gtcgccgccg ccgaaaccgc cgccacgatc gccgccgccg aagccgccgc gatcacgatc
       61 gccgaatccg ccgcgaccgc cgccgtagct gtcgccgcca tagggagccg gttcgaagct
      121 gtcgaaattg ccgaaatcat cgcgcttgcc gcgtccccga tggccacggc gatccttgtt
      181 gaaactcatt gcttagtagt cgctttcggt cgtccacacc ccattcaacc ggctccttgc
      241 gccggacggg gacgcagttc accacgggca cagactcgcc ctatgccaca aggacgcaag
      301 atatagccta attatccaca ggctgcgaac agaaaatttc agaaagacgg tgccggatat
      361 cgcacgcttt gccgcgggct tgcgcagccc ggcggcgggc ccgaaaacca gccattgagc
      421 ggcagggccg cttggcctaa ggcgacccta tgactgtcta tttccacgaa gaagatctgc
      481 ccgaaggcgt gctgcgcccg ggcccggtcg cgatcgatac cgagacgatg ggtttgaacc
      541 cgctgcgcga ccggctttgc ctcgtccaga tcagcgacgg gtcgggcgac gagcatctgg
      601 tgcgcttcaa gcccggaagc gcctatgacg cgccggtgct gaaggcgatc ctgaccgatc
      661 ccgagcgttt gaagctgttc catttcgcgc gtttcgacat cgcggcactg caacaggcgc
      721 tcggcgtcgt gaccgcgccg gtctattgca ccaagatcgc ctcgaagctg gtgcgcacct
      781 acaccgaccg ccacgggctc aaggaactgg tgcgcgaact gctcggccag gaagtgtcga
      841 agcagcagca gtcgagcgac tggggcgccg ccgagctcag cgacgcgcag cgcgattatg
      901 ccgcgagcga tgtgcgcttc ctgcacgcga tgaaggcgat tctcgacgcg cggctggcga
      961 gggaagggcg caccgccctc gcccaagcct gtttcgattt cctgccgacg cgcgccgcgc
     1021 tcgaccttgc gggctggccc gaagtcgata ttttcgcgca tagctgaagg tccgggaggc
     1081 tccgcccatg tccgaacgcg ccgatctcga tcgcacgatg cgccgcatgt gggcggcgag
     1141 cgggtcgagc cacgaccgcg tcgtgcgcat cctgcgcttc gggctgccgc tcgtcatcgg
     1201 cgtcgtcgcg gcggtgctcg tcttctcgcc cttcacccag cgcgccgaga tcagcttcct
     1261 gctcgccaag gacaaggtcg acatggcgag cgagcggatg aaggtgacgc gcgccgaata
     1321 tcgcgggcag gatgcgaagg ggcagccctt cgcgctgctc gcgggcagcg cggtgcagaa
     1381 aagctcggcc gaacccatcg tacggatgac cgacctgtcg ggcgcgatca agctcgccga
     1441 cgggccggcg acgatcgtcg cgaagagcgg cgcctataat atggagaccg aaaaggtcgc
     1501 ggtgccggga acggtgcaag tcaagagcgc gaacggctat agcatcgacg cgagcaatgt
     1561 cgcggtcgac ctcaacacgc gcacgctctc gggcgagggc ggcgtcgagg gcacattgaa
     1621 catcggccgc ttttcggcca atcggctgac ggccgacctc gacaagcgga tcgtgcggct
     1681 cgaaggcaat gcgcggctca ggatcaatca gggagtgctc aaatgaaccg gctttggacc
     1741 ctgaggagca tggcgcttgc gggcgcgggc ttcgcgctga ccagcctggc attcctgtcg
     1801 gccggcggcg gcgacgccgc gcaggcgcag gcactcgcca atcacaacag caacgcgccg
     1861 gtcgatttcg cggcgggtag catcgaggtg caggaccgcg ccgaccgcgt cgtgctggcg
     1921 ggcaatgtcc gcgtgacgca ggcggggctg acggtcaccg cgccgcggat gaccgtcgcc
     1981 tatacgcgcg cgggcggcac cgacgtcaac cggctcgacg cgaccggcgg ggtgacggtg
     2041 gtgaagggtg acgagatggc gaagggcaat gtcgccatct atgacctcga ccggcgcctg
     2101 atcacgatgg tcggcaatgt cgaactgcgc cagcgcggca acaatctgcg cggcgggcgg
     2161 ctcgtcatcg acctgaacag cggccgcgcg ac
//
LOCUS       DATMSU010000278         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_99696, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000278 DATMSU010000000
VERSION     DATMSU010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99696"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VN034_07500"
     CDS             complement(<1..236)
                     /locus_tag="VN034_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HWV60480.1"
                     /translation="MADGENGAGALGPVDIRRILTLLPHRYPMLLVDRVESMVKDTSI
                     HAVKAVTMNEPFFQGHFPGRPIMPGVLIVEALAQA"
     gene            complement(242..931)
                     /locus_tag="VN034_07505"
     CDS             complement(242..931)
                     /locus_tag="VN034_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HWV60481.1"
                     /translation="MKKIFAASALAIAALSVSPILSAPALAQAKGVGVADVRIAAARS
                     TAFQTASKQIETTYKAQIDQQQSRGQTLQAEINVLIAKYNEEAKKTPQNQAALQAAAK
                     AVQDKRQAAQQELGQIGAPVDLAIAYVEDQISVRMNEAIKAAMTAKRVDLLLNPDAVL
                     ARENNVDITDAVVTELNRILPSVSTAVPAGYQPGQLVQQRNQQLMDQARAAQGAATPA
                     PAPTGTQPTTR"
     gene            complement(931..3627)
                     /gene="bamA"
                     /locus_tag="VN034_07510"
     CDS             complement(931..3627)
                     /gene="bamA"
                     /locus_tag="VN034_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWV60482.1"
                     /translation="MNSVASHKFSARTQLSVVLLAGTMLATPLMAQEVAPPPVPAPTV
                     PAPAPEAVPAATTIQSITVVGNQRLEAQTILSYLRLRVGQQYDRAVLDQALKDLAATE
                     LFKDFQITDNNGALTIQVAENPVINRVILEGNKRLKEDKIRPEIKMAPRQIYTRSKVR
                     ADVARIIELYKRQGRFAATVEPKMVSLDQNRVDVVFEINEGPKSKVRQINIIGNDQFS
                     DGDLKDEMATKEKGLLTILSSNTTYDPDRLAYDQQKLKLFYLTNGYADFRVISAVAEL
                     TSNKQDFIITYVVEEGERYKFGDVDVKSEIRDFQPEMLKKLLPMKTGDWYDGKLVEDT
                     IESLSETAGLFGYAFADINPEFRRNPDDRTMAITFNVGESPRTYVERIDVNGNTLTHD
                     KVVRREFRLNEGDAFNSFGVKRTENRINSLGYFQENLEIQRKEGSAPDRIILETNVEE
                     KPTGELSLSAGFSSIENFLLQASIRQRNFRGLGQQLQASVNYSSYSKSIELGFTEPYL
                     FDRNISIGGTVYRRDLNSFNFIDNDRRTTFEQVTTGAQINVGVPLTEFMSFFGRYSIN
                     FDDVTLDKGIYYFGDECDPLVAGRYLCDAIGSRTTSLIGYTLAYDDRDNRLRPTRGQS
                     LSLSQDFAGLGGSVKYVRTRLAASKHFNLGSRFILNISAEGGYIYPFGGRPTPTSDKV
                     RLTDRFFLGEPQMRGFDIRGVGPRVIRYAAVYDPSNPVIDTSNDNRGQIDDALGGRAY
                     YQGRIELDIPLGTGAKELGLRPSIFLDVGSVFSVKRPTLTTLEDFRDTREGVPGSGLY
                     KFLCRNATTGQSQFATETTTTTGGVTTGTGDYTTCPEGFSSLAPFEERFFGDTWMPRV
                     AIGAGVNWNSPFGPFRIDFAYALRKEEGDDTKRFSFNVGTQF"
BASE COUNT          638 a         1093 c         1217 g          760 t
ORIGIN      
        1 gcctgcgcca gcgcctcgac gatgaggacg ccaggcatga tcggccggcc ggggaaatgg
       61 ccctgaaaaa agggctcgtt catcgtcacc gccttgaccg cgtggatcga ggtgtccttc
      121 accatcgatt ccacccggtc gacgagcagc atcggataac gatgcggcaa cagcgtcaga
      181 atccggcgga tgtccaccgg acccagcgcc cctgccccat tttccccgtc cgccatgacg
      241 cttagcgcgt ggtcggctgg gtgccggtcg gtgccggagc gggggtcgcg gctccctgcg
      301 ccgcgcgggc ctgatccatc agctgctggt tgcgctgctg gacgagctga cccggctggt
      361 agccggccgg gaccgcggtc gacacactcg gcaggatacg gttgagttcg gtcaccaccg
      421 cgtcggtgat gtcgacatta ttctcgcgcg ccagcaccgc gtcggggttg agcagcaggt
      481 cgaccctctt ggcggtcatc gcggccttga tcgcctcgtt catgcggacg ctgatctggt
      541 cttcgacata ggcgatggcg aggtcgaccg gcgcgccgat ctggccgagt tcctgctgcg
      601 ccgcctggcg cttgtcctgc accgccttgg cggccgcctg cagcgcggcc tggttctgcg
      661 gggtcttttt cgcttcttcg ttatatttcg cgatcaggac gttgatttcg gcctgcaggg
      721 tctggccgcg cgactgctgc tgatcgatct gcgccttgta ggtggtttcg atctgcttcg
      781 atgcggtctg gaaagcggtc gagcgcgcgg cggcgatgcg gacgtcggcg acgccgacgc
      841 ccttcgcctg cgcgagagcg ggagccgaca ggataggcga aaccgacagc gcggcgatcg
      901 ccagcgcgga agcggcaaag attttcttca tcagaattgg gttcctacat tgaatgagaa
      961 gcgtttggta tcatcgccct cttctttacg aagggcgtaa gcgaaatcga tccggaaggg
     1021 accgaaagga gagttccagt tgaccccggc gccgatcgcg acgcgcggca tccaggtgtc
     1081 gccgaagaag cgttcctcga acggcgcgag cgacgagaaa ccttcggggc aggtcgtata
     1141 gtcgccggtt cccgtcgtga ccccgccggt cgtcgtcgtc gtttcggtcg cgaactggct
     1201 ttggcccgtg gtcgcgttgc ggcacaggaa tttatagaga ccgctcccgg gaacgccctc
     1261 gcgcgtgtcg cggaaatctt cgagcgtcgt cagcgtgggg cgcttcacgc tgaacaccga
     1321 accaacgtca aggaagatcg acggccgcag ccccaattcc ttcgccccgg tgccgagcgg
     1381 gatatcgagt tcgatccgcc cttgataata ggcgcgcccg ccgagcgcat cgtcgatctg
     1441 gccgcgattg tcgttgctgg tgtcgatcac cggattgctc gggtcataga cggcagcata
     1501 gcggatcacg cgcggaccga cgccgcgaat gtcgaaaccg cgcatctggg gttcgccgag
     1561 gaagaagcgg tcggtcaggc ggaccttgtc gctggtcggg gtcggacgcc cgccgaaggg
     1621 atagatataa ccgccctcgg ccgagatgtt gaggatgaag cggctgccga ggttgaagtg
     1681 cttcgacgct gcaaggcgcg tgcggacata tttgacgctg ccgccgagcc cggcgaaatc
     1741 ctggctcagg gacagcgatt ggccgcgcgt cgggcgcagg cggttgtcgc gatcgtcata
     1801 ggcgagcgtg tagccgatca gcgaagtcgt gcggctgccg atcgcatcgc acagataacg
     1861 gccggcgacg agcggatcgc attcgtcgcc gaaataatag attcccttgt cgagcgtcac
     1921 atcgtcgaag ttgatgctat agcggccgaa gaaggacatg aattcggtga gcggcacgcc
     1981 gacgttgatc tgcgcgccgg tcgtgacctg ttcgaaggtc gtgcggcgat cattgtcgat
     2041 gaagttgaac gagttcaaat cgcggcgata gacggtgccg ccgatcgaga tattgcggtc
     2101 gaacagatag ggttcggtga agccgagttc gatcgacttc gaatagctcg aatagttgac
     2161 cgaggcctgg agctgctggc cgaggccgcg gaagttgcgc tggcggatcg aggcctggag
     2221 caggaaattc tcaatcgacg agaaaccggc cgacagcgac agttccccgg tcggcttttc
     2281 ctcgacattg gtttcgagaa tgatgcggtc gggggcgctg ccttccttgc gttggatttc
     2341 cagattttcc tggaaatatc ccaagctatt gatgcggttc tcggtacgct tgacaccgaa
     2401 gctgttgaag gcgtcgcctt cgttgagacg gaattcgcgg cgcacgacct tgtcgtgggt
     2461 cagcgtgttg ccgttgacgt cgatccgctc gacgtaagtc cgcgggcttt cgccgacgtt
     2521 gaaggtgatc gccatcgtcc ggtcgtcggg attgcggcgg aattcggggt tgatgtcggc
     2581 gaaggcgtag ccgaacaggc ctgccgtttc gctgaggctt tcgatcgtgt cctcgaccag
     2641 cttgccgtca taccagtcgc cggtcttcat cggcagcagc ttcttcagca tttcgggctg
     2701 gaagtcgcgg atttcgctct tcacgtcgac gtcgccgaac ttgtagcgtt cgccttcctc
     2761 gaccacatag gtgataatga agtcctgctt gttgctcgtg agctcggcca ccgccgaaat
     2821 cacgcggaaa tcggcatagc cgttggtgag atagaagagc ttgagcttct gctgatcgta
     2881 agcgaggcga tcggggtcat atgtggtgtt cgacgacagg atcgtcagca gacccttttc
     2941 cttcgtcgcc atctcgtcct tgaggtcgcc gtcgctgaac tggtcgttgc cgatgatgtt
     3001 gatctggcgg accttcgatt tcggcccttc gttgatttcg aagacgacat cgacgcggtt
     3061 ctggtcgagc gacaccatct tgggctcgac ggtcgcggcg aagcggccct gccgcttgta
     3121 gagttcgatg atgcgcgcaa cgtcggcgcg gactttcgag cgcgtgtaga tctggcgcgg
     3181 cgccatcttg atctcgggac ggatcttgtc ttccttgagg cgcttgttgc cctcgaggat
     3241 cacgcgattg atcaccgggt tttcggcgac ctggatcgtc agcgcgccat tgttgtcggt
     3301 gatctggaaa tccttgaaca gctcggtcgc ggcgagatcc ttcagcgcct ggtcgagcac
     3361 cgcgcggtca tattgctggc cgacgcgcag gcgcagatac gacaggatcg tttgcgcctc
     3421 gagccgctgg ttgccgacga cggtgatcga ctggatcgtc gtcgcggccg gaaccgcttc
     3481 gggcgccggc gccggaaccg tcggcgcggg gacgggcggc ggcgcaactt cctgcgccat
     3541 caacggcgtc gcgagcatcg tgccggccag aagaaccacc gacagctgcg tccgcgccga
     3601 aaatttgtgt gaagccacgc tgttcatccc gaaaaaataa ctcaaaagcg acaaacccgc
     3661 ctgaccgcgg gttcgtgcgc cactccctgc cccagactcg ctagccaa
//
LOCUS       DATMSU010000279        22484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_137539,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000279 DATMSU010000000
VERSION     DATMSU010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 22484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22484
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137539"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..290)
                     /locus_tag="VN034_07515"
     CDS             complement(30..290)
                     /locus_tag="VN034_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60483.1"
                     /translation="MPKPQSPVENPPNDVECIALVRPGSAMARRWNFSRPTFGIYEYS
                     KAANKHSLRFGDGSWQDLSVAMFPDVILLQDDGADLVGRLFD"
     gene            complement(391..1005)
                     /locus_tag="VN034_07520"
     CDS             complement(391..1005)
                     /locus_tag="VN034_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60484.1"
                     /translation="MTHTLDLDAMSPEEFAAFLDQLDRAEKLQTPEQIWRAAYREEQR
                     RKRILGYQPDLFGGEVVTHFRRNGPAPDFERPPIEELAAKLARIEGDPLAAFGPPRAD
                     RPAFLSEDEKAAVISGAANWLYVGQRVRIIDGPGPVDPQYGPYRFIGREGVVWRLCSS
                     VFADHCRVFLDPVRAERAEKIVFIEVRDIEPVASSGSAQPMRPG"
     gene            complement(1002..1595)
                     /locus_tag="VN034_07525"
     CDS             complement(1002..1595)
                     /locus_tag="VN034_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60485.1"
                     /translation="MRFTPHQGIYAYERTNRKLKAAERRLRLDREKFPLFSAEIAESQ
                     PTPEELLDARGRAFVANQQDNRDREARNWWRARAELRAIPETDRAAFVRYWDRCKCPG
                     NATYLLTYINMFRNGRLIVHEGEVRPRSDVEWERDRKAKIAAMTDLDLDVMIQTHISP
                     LFAEWGRVERRRRATAGECPKPDRARTAKRRGRGGRR"
     gene            complement(1703..2008)
                     /locus_tag="VN034_07530"
     CDS             complement(1703..2008)
                     /locus_tag="VN034_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60486.1"
                     /translation="MDVEILAHPKVKSALNRLIDLARSDTGQSARVTNFLLSWWDGDQ
                     WGHFPLTDLFAVDRDVAADMATVFEFLGQHGGAVYIDAFGDQYRDQMADLVERWRPD"
     gene            complement(2061..2405)
                     /locus_tag="VN034_07535"
     CDS             complement(2061..2405)
                     /locus_tag="VN034_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60487.1"
                     /translation="MRLYLTSTGQWSGTQSDAAGLVRANGGTWEQADVPTDKPGLIAW
                     LNDRQAGAAPVTAPPASKPTDADAQRAESLRRISIEEEIQGCDLPRLAVLAENVAWRF
                     HELARDVRRHHP"
     gene            complement(2433..3401)
                     /locus_tag="VN034_07540"
     CDS             complement(2433..3401)
                     /locus_tag="VN034_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HWV60488.1"
                     /translation="MARFDSFTDLAEHYASLTSADAFAGAFTSEITASQLSIHDEPTA
                     DDLPDANAARIVAAHVTGEIFNLFADTRLAAIAPRIAWGIVNSFHKVAQQLSRQEDDA
                     ARELGELARIMDPSEIHAVKIEEAQRLCQSLHEAIAAIEAMRDHAAEVYRVENGHPWS
                     ATTGSRTSRALTASQIDARDFLAARAAEKREAHAPSGPVVVLSGGQDWSEHQAIWDRL
                     DVIKSRIPAMTLVTTAQHKGADAIGAAWAAARGVPLVAFRLNRQLGNRAGFERNRSLV
                     RLLPVEGVICEGSGLQINLAQGLRAAGVPLTIFKHEDFAIRDQAHA"
     gene            complement(3515..4009)
                     /locus_tag="VN034_07545"
     CDS             complement(3515..4009)
                     /locus_tag="VN034_07545"
                     /inference="COORDINATES: protein motif:HMM:NF017128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 family protein"
                     /protein_id="HWV60489.1"
                     /translation="MANIGYINKTDEGGLVGKIDTLAFSATIGLRRFMSDNPKAPKYD
                     VMALTPTRTWVKIGALFEQSIRSSGESFYQGRIDDPSMSRPMDIALFAKEEGGYDIAW
                     TRRRARQDAPDSGEPAVPPMDDAAPEAPSGGLGDSTAPDAPQGGKGKGQAPKGGETAD
                     KQTA"
     gene            4656..5459
                     /locus_tag="VN034_07550"
     CDS             4656..5459
                     /locus_tag="VN034_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60490.1"
                     /translation="MPTSNMLDGGPHNPVSSQKSQSHSSVASNQTISAYFPSFSRQRG
                     VGEAVLPVWHTTPLSWYIAPAQYLILSTSVVRGPCRRRPNNSRIKTMIVTTSLSRRGL
                     VRAGGGLLLTGILPIDDNVSEWGRALQGRGAERFERAEEFYRGLAAGLYRDPRDLLYQ
                     AGIVAQLGIGAYLLELGASDDWCRQRIGLFVDKGLAIANLAGLNHRQPDMVQLARLLS
                     PYGQWRGPYAANLPAIGAIDPPRVSATLADLLMAVGHQLSGRRIEGHTR"
     gene            5474..5743
                     /locus_tag="VN034_07555"
     CDS             5474..5743
                     /locus_tag="VN034_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWV60491.1"
                     /translation="MSRAYWLWIRAKRYPQYARRLGSDAPILDQLDLAMDLLWPFARR
                     VFLMHRVDELPYGVIAIAVDVDVATVERCVADALWSVRMVRREFE"
     gene            5879..6388
                     /locus_tag="VN034_07560"
     CDS             5879..6388
                     /locus_tag="VN034_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60492.1"
                     /translation="MERRTLMGGLLMASGFVGVSRLGAERQPCDDDGNENALWRLQNA
                     RSFFPNREFACPGDLFYQLGIVSQLALTACVIADGWSDEDCRRRIGQDVGKAHFYAKA
                     KGLHFNSDRFARLAPLLSPYGRWRSPATGDVVDVGKINAAEAKSAVLQLLIAVRSHLV
                     GAEGRAPSR"
     gene            6385..6684
                     /locus_tag="VN034_07565"
     CDS             6385..6684
                     /locus_tag="VN034_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60493.1"
                     /translation="MMWHHRIARVYWLHVRLRHYPAYARRIGTSPDIREQVKLAIQLI
                     WPLARSVYLLNCVHELSYRQIATCVGVDIRTVELCIADALISLWTICDQFGETPR"
     gene            6807..7379
                     /locus_tag="VN034_07570"
     CDS             6807..7379
                     /locus_tag="VN034_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HWV60494.1"
                     /translation="MTIFNVRLSREDEAELAAAAKKSGRSKSEIARDAISAHLMNMAD
                     DERRWETLQDDWSEYGAAVQDAYEALVGVQVAKLDAVRKAFEARLVEIGNMDWAQAIE
                     PVYEIFRRKVIEKSGVAYAPNPYIGGLDPKEPEVVAVIGFAADDTHDFVIPLEQVFYN
                     YCDSAGWDAFNEIPSVYARARARLEADEKK"
     gene            7477..7977
                     /locus_tag="VN034_07575"
     CDS             7477..7977
                     /locus_tag="VN034_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60495.1"
                     /translation="MALQDLWLEGIDIDAALYDVRDDDSQAWETRALAGASIGIDAIA
                     GFRAVLELCEAMNRIVRGEDDGKRRLAEILGRDGDDYQRCLWYSVAGRDPLAVATCFS
                     ELEKLMAARASLWVEASRRGLVPSKTDNPYWTSSPEGPRAKFDAKFELGEHWTPFLPT
                     ELLQED"
     gene            7980..9209
                     /locus_tag="VN034_07580"
     CDS             7980..9209
                     /locus_tag="VN034_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1173 domain-containing protein"
                     /protein_id="HWV60496.1"
                     /translation="MQRYYVIDREIAAGEHGFDEMVARAYTLKQAVRCLCRRDIELDL
                     YIAHRHGGHVLSRWPGTGPRHAPNCDHYEAPDHLTGLGQVLGSAIVDDIDNGVTTLKF
                     GFPLSKGPARAAPASFTNDKPDVKATGQRLTMRGLLHFLWDRAQLTHWHPRMAGKRNW
                     YIVRRQLLNAALGCKVRGDSLAPRLFIPETFRLDDKDGIVGRQHAELALARSTPDHIM
                     LLIGEVRSIETARFGEKILIKHLPNFPFLMDADMARRFHKRFAAEEELWRSDDRDGHL
                     MIAASFAMGASGLAQIFEMSVMPVTREWLPYEGLEERSLVTHAVEDQRHFVKGMRVNL
                     GLEMPIASLTLTDTGTEATAVYLAHNLPEPRYDEALAQLMKTQGVQHMTWRPGDRLQV
                     AQGAGQSIVVGGTLTAH"
     gene            9227..9769
                     /locus_tag="VN034_07585"
     CDS             9227..9769
                     /locus_tag="VN034_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60497.1"
                     /translation="MAIVDKMTAAERLMQNAVDMIDRKDDPLAIHVVASSALSLLREL
                     VGTSGDDYVTQLMKEALFRAAQAKLQGLPTGMPEAESIDHIVETVVRGIEESGWKSAA
                     DVVIVTPKKEVRAWLYYIFKPYNFLKHADQDPLATLDEGDFDPGGALAHAMTAYLMAR
                     GDGKLPEPFTVFLKKQGILV"
     gene            complement(9732..10016)
                     /locus_tag="VN034_07590"
     CDS             complement(9732..10016)
                     /locus_tag="VN034_07590"
                     /inference="COORDINATES: protein motif:HMM:NF022642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MW1434 family type I TA system toxin"
                     /protein_id="HWV60498.1"
                     /translation="MDFREALDEIENGARVRREGWHKGRRWIARQQKDGFAEYVMAHA
                     QDDQTIEWPLAMGRADWCATDWIVVDDSGADSLAPPAVATPESPVSSETP"
     gene            complement(10115..11137)
                     /locus_tag="VN034_07595"
     CDS             complement(10115..11137)
                     /locus_tag="VN034_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60499.1"
                     /translation="MFCVQHRPIEDAPVVVAFGAGVDSTAMLLEMVAQGRRIDLVLFA
                     DTGAEKPETYAYRDLIFRWLRARGIRSETVRYEPRRFKHWPPYRTLDENCFTNGTLPS
                     ISFGRHSCSQKWKIEPQDRFVQSWEPALEWWARGGKVTKCIGYDAGDRDSKRYAHAEG
                     HLDPRYQYEYPLREWGWDRAACEKRILSEGLPLPVKSACYMCGAAKPWEIRGLPIFQL
                     RRIVLMEARAKPRLRNVEGLWRKSVKGRNGAERRPGAMTDFIRDERLLPPDEIDAIIA
                     MAPAALLRFQDAEGQFALDKRAELSAWLKWFDANDMRMFDAPTGPRFYAGIVEPPIGR
                     PLCDAA"
     gene            complement(11161..11364)
                     /locus_tag="VN034_07600"
     CDS             complement(11161..11364)
                     /locus_tag="VN034_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60500.1"
                     /translation="MRTLATFENAYRLAVMLSDIDGAPFVIVATSNIRQPHRVERAAG
                     QPNAIAFITTADAQLEISPALAS"
     gene            complement(11436..11798)
                     /locus_tag="VN034_07605"
     CDS             complement(11436..11798)
                     /locus_tag="VN034_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3768 domain-containing protein"
                     /protein_id="HWV60501.1"
                     /translation="MQTDENTTSRTATIARMNDDARLGLDRRARITFTRNCIETFRST
                     PETHPAIVRAQLLKAFRHCEFAADSPEHDFASILYQDRKVWLKIDYYDLACEYGSEDP
                     ANAAITTRVITIMLPEDY"
     gene            complement(11804..12058)
                     /locus_tag="VN034_07610"
     CDS             complement(11804..12058)
                     /locus_tag="VN034_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60502.1"
                     /translation="MIEPMLVLSTGNLTFETCNRWMKKADHAVFEKGEYGWFVYVGEP
                     GDKLPADLLDCIALARQRHCCWVMFDRDAPASAELPIYDW"
     gene            complement(12055..12264)
                     /locus_tag="VN034_07615"
     CDS             complement(12055..12264)
                     /locus_tag="VN034_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60503.1"
                     /translation="MTDHGERPFTEIGIAGGHLYLRVMRSFVSEFRGRNYISADAPLT
                     EINAPIGIMRRDLRVWLEHRAGDLP"
     gene            complement(12612..13517)
                     /locus_tag="VN034_07620"
     CDS             complement(12612..13517)
                     /locus_tag="VN034_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HWV60504.1"
                     /translation="MKPRHDIYSSITAQLVAAIEAGAGEWRMPWHHRGAPVMRPRSAA
                     GRAYSGINRLVLWASAETRGFESGTWATFQQWKSLDARVRKGETGTHVILWKRQERDE
                     TATADDQDRGGRFFARSFVVFNRSQVDGAGDEDEDVPPPMSERIAAAKAFLEHSGVPV
                     TFGLHDAYYRPDEDRIYMPARSAFESDEAMIATEGHELVHATGHASRLARDTLRDYHK
                     ERAIRAREELVAEIGASFLMADLGLAYSPRPDHAAYVASWLRALENDTRAIFRAAAAA
                     QAATDWIHSAVSAATANADPLPLAA"
     gene            complement(13514..14065)
                     /locus_tag="VN034_07625"
     CDS             complement(13514..14065)
                     /locus_tag="VN034_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60505.1"
                     /translation="MADRVAASITIGGSVTGADFITLCRLIAAENLSVEEDGDPFSPA
                     DWTDGEPLQLFDHQAIGGQFEALEAWCVQRGLPFVRWCAGYPGGWEPERVIFTGTGAI
                     ETYPATERGNAIATRDTLDLHENLATLRAWFDAAHFEIPPLDILADVGPTISNITRAS
                     AGQEESGGGGSEHCSQLEKGSAQ"
     gene            complement(14168..16219)
                     /locus_tag="VN034_07630"
     CDS             complement(14168..16219)
                     /locus_tag="VN034_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWV60506.1"
                     /translation="MQNQQIPFNKLRLSPTNVRKSFSQAGVDALAVSIDAYGLLQPII
                     VSPATDKKSVFDVHAGGRRWRAIAKLIESGQLPKNAMVDARVCNDEKAALAEEISLAE
                     NLIREAMSPADECRGYRAIMDKGANEEDVARRFGVTVRHVQGRLRLADLAEPIFIALA
                     EGKITLDVAKAYGSTSDRDTQLAAWNNFKDHWQGDQPHTIRRYVTESAIEATSAVAKL
                     VGEQEYLAAGGRVERDLFAGEGEGQWLDRGIAEDIARAKLQEAADDIMQLGTLGWVRV
                     VLAQHVPHNETDGLHDYYIRRGELSDADQARMTAIEERLQAIEEEIEDATDQDVVLRL
                     EAENEALDAEHTEIDKRSYVIPEDERPNVGTFIVLASDGTPKVSHRYFSTAAPKREKR
                     AATVTEDGAEPAAIENVFPRSLEEQLAKDRRDVLALHVAQDPGLALDLTIFRLARKFT
                     GHAAYNDTGVMVTIGELFDPTGFAAAQPTAAQDSLEAIRSGLPCDWAGFADSFDAFLA
                     FRELDEADKAAWLAFAVSRSLQASLATGDRKNSFQTQLGALLEIETADHWRPSAESFF
                     DKIRKPQILSILGKLDPELPGRYASEKKAALSSAAAKLCAGETIATPEVKERALSWIP
                     ETMQFRSAVTPNEPEVPPVDDDSAEEQPAIDDGGDAQADDLDPGVNDVAQHEDALAEA
                     A"
     gene            complement(16702..17163)
                     /locus_tag="VN034_07635"
     CDS             complement(16702..17163)
                     /locus_tag="VN034_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HWV60507.1"
                     /translation="MTETPQHDSDLLTLTADIVAAHVGNNSVAISDISILISNVHAAL
                     SGLSQPAEVPEEKPVPAVSIRSSVKPDYIVCLEDGKKLKMLRRHLMTHYGLTPDDYRS
                     KWGLPADYPMVAPDYAERRRVLAKEIGLGTKGRGGGRKPARAAAKPAAKRT"
     gene            complement(17307..17501)
                     /locus_tag="VN034_07640"
     CDS             complement(17307..17501)
                     /locus_tag="VN034_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60508.1"
                     /translation="MNDKVKIALIAALAALGVTWIIMHYSPYQTCVRAKQAMISEEVL
                     TEYTPEESLRLAQTQCAGAD"
     gene            complement(17611..21837)
                     /locus_tag="VN034_07645"
     CDS             complement(17611..21837)
                     /locus_tag="VN034_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="strawberry notch family protein"
                     /protein_id="HWV60509.1"
                     /translation="MLTDTDQAPDTAARLIDAARSIATQFDDDKAVRRGLINEAMTAA
                     FGAKSAEGAWTQRDSFVMTEISAILSARSVKLPDQPLAIINQLATLEKAFPTQTVRSE
                     EQIAHQHFSTPLDLAWLVNHLAIIEPEDVVLEPSAGIGTLAQWAGQGKTLQLNELDQV
                     RAAVLRNLFPECSVTTFNAAQINQHVSVRPTVVIMNPPFARNASGAEDPFAAHRHFVA
                     ALAALQHGGRIVAIMPDSFNPGGKHGSLFDRAVSGTTVRLMLRLDKGFAGKGTSVAVR
                     IIVADKETTGKRPPSTINRASASKLLEAIRAIPPRAKLQPDPEPPVRPRTTKPVALFG
                     AFRTAPAKSFPRPARPLPSFEIATLNYRVHDPVAAAHDEAAIYVPWRSQRLEFEHSSE
                     HPSPLVESAAMAAVALPAPSYRPLLPKRVLDDKILSAAQLETIVHALAATEIDLPGRY
                     RLPEKGIALVPHADGNCYRQGFFLGDGTGAGKGRQLAGIAMDQWLRGKRRHIWLSDSS
                     ALIEDARRDWQALGGTPLDIQPLGRVRPGAGIEIGDSILFASYATLRSEAGGDRRVDQ
                     ILAWLSEDFDGLILFDEAHAMGGVAGGEGRFGTISGSQQGLAGVELQNRLPRARVIYA
                     SATGASDVNNLAYATRLGLWGEGTAFVDREAFTARIRDGGVAAMELVARELKAMGVYT
                     ARALSYAGVEYDILEHELTPTQIVDFDTYADAWAIIHRNMEEALAAAGVVDRLTGATL
                     NGQALSAARSRFESAKQRFFSALLLAMKLPSLLPAIEAALQAEQSAVIQLVTTSEALL
                     SRRLADLDPADRAELALDLSPRELVMDYLEAAFPTRAMEIISDDDGNERSRPRSDVDG
                     NPVHSEEALQIRADTLELLGAMPPIAPALDAVIERFGTDAVAEVTGRTKRLVRLPDGS
                     QKLQSRSPSACMADADAFMNGAKRILVFSDAGGTGRSYHASLDHQNQQRRVHFLLEPG
                     WRADRAVQGLGRTNRTNQAQPPIFRPVTTDCRGERRFISTIARRLDALGALTRGQRQT
                     GGQNLFDPMDNLESDYAKDALLTWYRLLHRGKLTSVTFSDFCHRTGLRLEYEGELVDK
                     LPPIQRWLNRILALPIAIQNAIFDEYLGLVETRIDAAKKAGTFDAGVETIRADKLTIV
                     DEITLREGVDTSSSTRLLTIEAEWKREMCSLAEVSALADRFRSRAIRLRNARSGKVAL
                     QTPARTRLTDTGEAVATWQLSRPGHHQYITAGDLEESMWERVDVDTFGQCWTAECDEL
                     RLNPNRERFFLATGRLLPIWNLLGDDPEVRRLVTADGRALLGRIVPHGDVNTLLGKLG
                     IAGAIKLAPAEIVAAAWEGKTVMVGGNLGLTLERRRVNGEPRLELSGFDPRSLPTLKA
                     KGCFTEIIQFRTRLFVPVSRATEILAEIAQ"
BASE COUNT         4132 a         6461 c         7210 g         4681 t
ORIGIN      
        1 ctctacgccg cgatccactg ccgcatcggc tagtcgaaga ggcgcccgac gagatccgca
       61 ccgtcgtctt gcaacagtat gacgtccggg aacatcgcga cggacaaatc ctgccagctc
      121 ccgtcgccga acctcagcga atgtttgttc gcggccttgc tgtactcgta gatgccgaac
      181 gtaggcctcg agaagttcca gcggcgggcc attgccgagc cgggtctgac gagcgcgatg
      241 cactctacgt cgttcggcgg attttctacg ggcgattgcg gcttcggcat ggggtttcct
      301 cttgctctag tttcgattcc taggacggga tcacctcttc ccgtctccga atttgccgcc
      361 gccgggcctt taggaccggc ggcggcgcga ttatccgggc cgcatcggct gtgcgctgcc
      421 ggaagacgca accggctcga tgtcgcggac ttctataaag acgatcttct cggcacgttc
      481 cgcccggacc ggatcgagaa aaacgcggca atgatctgcg aacacggatg agcagaggcg
      541 ccacacgaca ccctcacggc cgataaatcg ataaggacca tattgcggat cgactgggcc
      601 gggtccgtcg ataattcgaa cgcgctggcc gacataaagc cagttggcgg cgccgctgat
      661 aaccgcggcc ttctcgtcct cgctcagaaa ggctggccga tctgcgcggg gtggtccgaa
      721 tgccgccagc gggtcgccct cgatccgcgc gagtttggcc gcaagttcct cgatcggcgg
      781 gcgctcgaag tccggcgcag gtccgttcct ccgaaaatgg gtcacgacct cgccgccaaa
      841 tagatcgggc tgatagccaa ggatgcgttt gcgccgctgt tcctcacggt aagccgcgcg
      901 ccaaatctgt tcgggcgtct ggagcttctc ggcccggtca agctggtcga ggaaggccgc
      961 aaattcttcg ggcgacatgg cgtcgaggtc gagcgtatga gtcatcgccg ccctccccgc
     1021 ccgcgccgct tggcggtgcg ggcgcgatcc ggtttggggc attcccccgc cgtcgcacgc
     1081 ctcctgcgct ccacgcgccc ccattcggcg aataacgggc tgatgtgcgt ctgaatcatg
     1141 acgtcgaggt caagatcggt catggccgcg atcttcgcct ttcggtcgcg ttcccattct
     1201 acgtcactcc ggggccgaac ctcaccttcg tgaacgatca ggcggccgtt ccggaacata
     1261 ttgatgtagg tcaggagata ggttgcgttg ccgggacatt tgcagcgatc ccaatagcgg
     1321 acgaatgcag cgcgatccgt ttcggggatc gcgcgcagct cggcgcgcgc gcgccaccag
     1381 ttcctggcct cgcgatcccg attgtcttgc tggttagcga caaacgccct gccgcgcgcg
     1441 tccagcaatt cttccggggt tggctggctt tccgctatct cggccgaaaa gagagggaat
     1501 ttctcgcggt cgagacgcag gcggcgttca gccgccttca atttccggtt ggtccgttcg
     1561 taggcgtaaa ttccctgatg gggtgtgaag cgcatggcaa tctatccgac gcagcgcggc
     1621 cacgctgggg gggatgctcc aacgcctgct gcaacaaaaa ggggtttgaa atgacgaagg
     1681 gctgccgcga gcggcgcgta acctagtcgg ggcgccagcg ctcaaccaga tcggccattt
     1741 ggtcccgata ctggtcgccg aaagcgtcaa tgtagaccgc gccgccatgc tgtcccagaa
     1801 actcgaagac agtggccatg tcggccgcaa cgtcccggtc gaccgcgaac aggtcggtca
     1861 acggaaaatg accccactgg tcgccgtccc accacgagag gagaaaattc gtgacgcggg
     1921 cagattggcc cgtatcggaa cgggcaaggt cgataaggcg gttcagcgcg gacttcacct
     1981 tcgggtgagc tagaatttcc acgtccatcg gttcggctcc tccagttcgt gtatcaagaa
     2041 aaatgagttt agtcagtcca tcagggatga tggcggcgca catcgcgcgc gagttcgtgg
     2101 aatcgccatg cgacattttc cgcgagcacc gcgaggcggg gaaggtcgca gccttgaatc
     2161 tcttcctcga tgctgatccg ccgcaaactt tctgcgcgct gcgcgtccgc gtcggtcggt
     2221 ttcgacgcgg gcggcgcggt caccggtgcg gcgcccgcct gcctgtcgtt gagccatgct
     2281 atcagtcccg gcttgtcggt cgggacgtcc gcctgttccc aagtcccgcc gttcgcgcgg
     2341 accagacccg ctgcatcgct ctgtgtgcct gaccattggc ctgtgctggt gagatagagg
     2401 cgcatcgaaa agctcctgac gggcgaaagc gatcaggcgt gagcctgatc gcggatcgca
     2461 aaatcctcat gtttgaagat ggtgagcggg acgccagcgg cgcgaaggcc ctgcgcgaga
     2521 ttgatctgaa gcccgctgcc ttcgcaaatg acgccttcga cgggaagcag ccggaccagt
     2581 gagcggttgc gctcgaaacc ggcgcggttg ccaagctggc gattgagccg gaaggcgaca
     2641 agcggcactc cgcgcgctgc ggcccatgct gcgccgattg catccgcgcc cttgtgctgc
     2701 gccgtggtga caagcgtcat ggcaggaatg cggctcttga tcacgtcgag acgatcccag
     2761 atggcttgat gctcgctcca atcctgtccg cctgacaaga cgacgacggg gccgctcggg
     2821 gcgtgggcct cgcgcttctc ggcggcgcgg gctgcaagga agtcgcgggc atcgatctgc
     2881 gaggcggtca gggcgcggct tgtccggctt cccgtggtgg ctgaccaagg gtgaccgttt
     2941 tcgacacgat agacttcggc ggcatggtcg cgcatcgcct cgatcgccgc gatggcttcg
     3001 tgaagcgact ggcaaaggcg ctgcgcttct tcgatcttca ccgcgtgaat ttcggaaggg
     3061 tccatgatgc gggcaagctc gcccagctcg cgcgcggcgt cgtcttcctg acgggaaagc
     3121 tgctgtgcga ccttgtggaa gctgttgacg atcccccaag cgatgcgcgg tgcaatggca
     3181 gcaaggcggg tgtcggcaaa gaggttgaaa atctcgccgg tgacgtgggc ggcaacgatc
     3241 cgcgcggcgt tggcgtcggg caagtcgtcg gcggtcggtt cgtcgtggat ggaaagctgg
     3301 ctggcggtga tttcgctggt gaaagcgccc gcgaaggcgt cggcgctggt gaggctggca
     3361 taatgttctg caagatcggt gaagctatca aagcgggcca tgttgaaaat tcctgttcgc
     3421 cgcttcggaa attcgataag catcgaagcg gacattgacc aatgttcagc catgagaggc
     3481 cggaagagct agaccctctc ccggcctcct ttattcaggc agtctgcttg tcggcggtct
     3541 cacccccctt cggagcctga cccttgccct tgccgccctg gggcgcgtcg ggtgcggtgc
     3601 tgtcgccaag gccgccgctc ggtgcttcgg gtgcggcgtc gtccatcggg ggcactgccg
     3661 gttcgccgct gtcgggggcg tcctgccgcg cgcggcgacg ggtccaagca atgtcgtatc
     3721 cgccttcttc cttggcgaag agcgcaatat ccatcgggcg gctcatgctc ggatcgtcga
     3781 tgcggccctg atagaagctt tcaccgctcg accggatcga ctgctcgaac aaagcgccga
     3841 tcttcaccca agtgcgggtg ggggtcagcg ccataacgtc gtacttcggc gctttgggat
     3901 tgtcgctcat gaagcggcgc aaaccgatgg tagccgaaaa ggccagcgta tcgatcttgc
     3961 cgaccaagcc accttcgtcg gttttgttga tgtaaccaat attcgccatg attttcactc
     4021 cagataacag ggcaatgccc gcttagttat tgtcaaaccg gcgtttccgt cttgaccatt
     4081 acttaatagc ggccctgtca aatctggtct atcgcattgt tttcttgact gaaatccgat
     4141 cctctttggt gagccggagc atcaaaatgc cgtgcgggag cgcggccgac aaagaagccc
     4201 ggtccgacca ggaccggatc catcctggtc gggggaagcg atgcccttcc ccacctttcc
     4261 atccccttcg gagccgccgt cgcaggcccc aagaagcaga cgggggatag cgcggggcaa
     4321 gctgaaaaac ggaggctccg ccgcgtagcg gtggaaaccg taggcccaca gggccggttt
     4381 tctgcttgag cgcccaaagg gcgcgtcccc gcgtgccgcc ggatgcgacg cagggagcct
     4441 gaaaggcggc gtagaagggc atggaaaggt atgcgcgcac accggcgccg cgacacgcga
     4501 atagcgtggc gcggccgaag agaatgacgg cggtcgccgg tcggcgatcg accatatcgc
     4561 cctgcccctg atcgtgaccc gcagggccga gacccaaagg ggctcggtga gctttggccg
     4621 cgatcgaaga tcggggcttt agcgaataga gcaggttgcc gaccagcaac atgctggacg
     4681 gcggccccca caatccggtt tcatctcaaa aatctcaatc acacagctcc gtcgcatcaa
     4741 atcagaccat ttctgcctat ttcccgtcat tttcgcgtca gcgaggtgtc ggcgaagccg
     4801 tgttgcctgt gtggcacacc accccccttt cttggtatat cgcgcccgcg caatatctga
     4861 ttttatccac ctcggtcgtt cgaggtccat gccggcggcg gccgaacaac agcaggataa
     4921 agactatgat cgtgacaacg agcttgtcgc gccggggcct tgtgcgcgcc ggcggcggac
     4981 ttttgctgac cggcatattg ccgatcgacg acaatgtgag cgagtggggg cgtgccctgc
     5041 agggccgcgg cgccgaacgc ttcgagcgag ctgaggagtt ctaccgtggc ctagccgcgg
     5101 ggctctatcg agatccccgc gacctgctct accaggcggg gatcgtggcg cagctcggca
     5161 tcggagccta tctgctcgag ctcggcgcat cagacgactg gtgcaggcag cggatcggtc
     5221 tgtttgtcga caagggactg gcgatcgcga accttgccgg attgaatcat cgtcaacccg
     5281 acatggttca actggcgcga ttgctctcac cctatgggca gtggcgtggt ccgtatgctg
     5341 ccaatctgcc agcaatcgga gcgatcgacc cgcctcgcgt gtcggcgacg ttggccgatc
     5401 ttcttatggc ggtcgggcat cagctcagcg ggcgacgcat cgaggggcac acgcgatgaa
     5461 attgcatcat cggttgtcgc gcgcctattg gctttggatc cgagcgaagc gctatcctca
     5521 atatgctcgc cggctcggtt ccgacgcgcc gatcctcgat caactcgatc tggcgatgga
     5581 tctattgtgg ccattcgccc ggcgcgtgtt tctcatgcac cgggtcgacg agcttcccta
     5641 tggggtaatc gcgatcgcgg tcgacgtcga cgttgcgacc gtcgaacgct gcgtggccga
     5701 cgcgctctgg tcggtcagaa tggtccggcg ggaattcgag tgaagcaccg aattctattc
     5761 accgttgctc tcatgttacc cccatttttt ggagtacgat tttacagggg gtgatttggc
     5821 cgccgcaaac ctgatcctac tcgcgcgcac aagcagcgcc gagcatggag gactctggat
     5881 ggaacgtcgg acactgatgg gtggattgct gatggcttcg ggcttcgtcg gcgtaagtag
     5941 acttggcgcc gaacggcaac cttgcgacga cgacggaaat gaaaatgcgt tgtggcgcct
     6001 acagaacgcg cgatcgtttt tcccaaatcg agaattcgcg tgccccggcg acctgttcta
     6061 tcaattgggc attgtgtcgc agctagccct gacggcgtgt gtcatcgcgg atggatggag
     6121 cgacgaggac tgtcgccgca ggatcgggca ggacgtcggc aaagcccatt tctatgcgaa
     6181 ggccaaaggg ctgcatttca attctgatcg cttcgcgcgt ctggccccat tgctgtcgcc
     6241 atatggtcgg tggcgatcgc cggcgacggg agatgtcgtc gatgtcggaa agatcaacgc
     6301 cgccgaggca aagtccgcgg tgcttcaatt gctgatagca gttcggtcgc acctggtcgg
     6361 cgctgaaggc agggctccaa gccgatgatg tggcatcatc gcattgcacg agtctattgg
     6421 ctgcacgtcc gattgaggca ttatccggcc tatgcgcggc gtatcggcac atcgccggat
     6481 atccgggagc aagtgaagct cgcgattcaa cttatttggc cgctggcgcg gtcagtctat
     6541 ctcctgaact gcgttcacga actaagctat cgccagatcg cgacgtgcgt cggtgtcgat
     6601 atccgtacag tcgagttgtg catcgccgac gcactcattt cgctgtggac gatctgcgac
     6661 cagtttggcg agactccccg ctaaattcgg gcgatcattg tcgcgctggc ggtgcattac
     6721 atgccggtcc acgagacgtg taatacaaac ttgcagactt tccaaacttg tcttacgcat
     6781 gtccgacttg tcatcgggag atgcgtatga cgattttcaa tgttcggctt tcgcgtgagg
     6841 atgaggcgga gctggccgcc gccgcgaaaa agtccggtcg cagcaagtcc gagatcgcgc
     6901 gtgacgcgat ttcggcccac ctgatgaaca tggccgatga cgaaaggcga tgggaaacgc
     6961 tccaggacga ttggagcgaa tatggggccg ctgtgcagga cgcctatgaa gctctcgttg
     7021 gtgtccaggt tgccaagctg gatgcggttc gcaaagcatt cgaggcacgt ctggtcgaga
     7081 tcgggaatat ggattgggca caggcgatcg agccggtcta cgagattttt cggcggaagg
     7141 tgatcgagaa gtcaggggtc gcatatgccc cgaaccccta catcgggggc cttgacccca
     7201 aagaacctga ggtagttgcc gtgatcggtt ttgcggccga cgatacccat gactttgtga
     7261 tcccgcttga gcaggtattc tataactatt gcgattcagc gggctgggac gccttcaacg
     7321 aaattccgtc ggtatatgcc cgggcgcggg cgcggctcga ggcggacgag aagaaatgag
     7381 ttcggcccaa gtcgatttag ggattcgcag ggacgatcca gttgataata tgccgatctt
     7441 cgaattgtgg tcggctcaga agcgaggagc aatttaatgg cgctgcaaga tttatggctc
     7501 gaaggcatcg atattgacgc agcgctctac gatgtccgcg acgatgatag tcaggcatgg
     7561 gaaactcgcg cgcttgccgg cgcgagcatt ggcatcgatg caatcgccgg tttccgcgca
     7621 gtcctggagc tttgcgaggc catgaatcgg atcgttcgcg gcgaggacga tggcaagcgc
     7681 aggcttgccg agatcctcgg ccgcgacggt gacgattatc agcgttgtct ttggtactcg
     7741 gtcgcaggcc gcgatccgct ggccgtagcg acctgcttca gcgagctgga gaagctgatg
     7801 gcggcgcggg ccagtttgtg ggtcgaagcg agccgccgtg ggctggtccc gtcgaagacg
     7861 gacaatccgt attggacgtc aagcccggaa ggcccacgcg cgaagtttga cgccaagttc
     7921 gagctcggtg agcattggac gccgtttctg ccgacggaat tgcttcagga ggactgatca
     7981 tgcagcgcta ctatgtgatc gaccgggaga ttgccgcagg cgagcatggt ttcgatgaga
     8041 tggtggcgcg ggcctatacg ctgaagcaag ctgtccgttg cctatgccgg cgcgatatcg
     8101 agctcgatct gtatatcgcg catcggcacg gcggtcatgt tctgtcgcga tggcccggaa
     8161 cgggcccgcg acatgcgccg aattgtgatc attatgaagc accggatcat ctgacggggc
     8221 ttggccaggt cctcggcagc gccatcgtcg atgatatcga caatggcgtg acgacgctga
     8281 aattcgggtt tccgctgtcg aaagggcctg cgcgcgcggc gccggcgtca ttcacgaacg
     8341 acaagcccga cgtgaaagcg acgggtcagc gcctgacgat gcgcgggctg ctacattttc
     8401 tgtgggatcg tgcgcagctc acgcattggc atccgcgcat ggcgggaaag cggaactggt
     8461 acatagtgcg ccggcagctg ctgaatgcgg ccctcggctg caaggtgcgc ggagattcgt
     8521 tggcgccgcg gcttttcatc ccggagacat tccggctcga tgacaaggat ggtatagtcg
     8581 gccgtcaaca tgccgagctc gctttggcga ggtcgacgcc ggaccacatc atgctgctga
     8641 ttggcgaggt ccgttcgatc gagactgctc gcttcggcga aaagatcctg atcaagcatc
     8701 tgccgaattt tccgttcctg atggatgcgg acatggcgcg ccgttttcac aagcggtttg
     8761 cggccgaaga ggagttgtgg cgctcggatg accgggatgg tcatcttatg atcgcggcga
     8821 gttttgccat gggagcctcc gggctcgcac aaatcttcga gatgtcggtg atgccggtga
     8881 cacgcgagtg gcttccatat gaaggtctgg aagagcgttc gctggtcacg cacgcggtgg
     8941 aggaccagcg acatttcgtg aaggggatgc gtgtgaatct cggactcgag atgccgatcg
     9001 cgagcttgac cctgaccgac accggcactg aggcgactgc ggtgtatctc gcgcacaatc
     9061 ttcccgaacc gcgctacgat gaagcgttgg cgcagttgat gaagacccaa ggcgtccagc
     9121 acatgacttg gcgcccgggc gatcgtcttc aggtagcgca gggtgctggg caatcaattg
     9181 tcgttggcgg cacgctgacg gctcattgag cgatcgaggg ggcgtaatgg caatcgtcga
     9241 caagatgacg gccgcggagc ggctgatgca aaatgcggtc gatatgatcg atcggaaaga
     9301 cgatcctctc gcaattcatg tcgttgcatc gtcggcgctg agcctcttgc gcgaattggt
     9361 tggcacgagc ggcgacgact atgtgaccca gctgatgaaa gaggctctgt ttcgcgcggc
     9421 gcaggcaaag cttcaaggat tgccaacggg gatgcctgag gcggagtcta tcgaccatat
     9481 tgtcgagacc gtagtgcgcg ggatcgagga gagcgggtgg aagtctgcgg ccgacgttgt
     9541 aatcgtgacg ccgaaaaaag aggtcagggc ttggctctac tatattttca agccctacaa
     9601 tttcctgaag cacgctgacc aagatcccct ggcaacgctc gatgaaggcg atttcgatcc
     9661 ggggggcgcg ttagctcatg ccatgactgc ctatttgatg gcgcgtggcg acggcaagct
     9721 cccggagccg tttacggtgt ttctgaagaa acaggggatt ctggtgtagc gactgccggc
     9781 ggtgcaaggc tatcagcgcc gctatcgtca acaacgatcc agtcggtcgc gcaccaatcc
     9841 gctcgtccca tcgcgagtgg ccattcgatc gtctgatcat cttgtgcgtg tgccatcaca
     9901 tattcggcaa acccgtcctt ttgttggcgt gcaatccacc ggcggccttt atgccagcct
     9961 tcgcgtcgta ctcgggcgcc attctcgatt tcgtcgagtg cttcacggaa gtccatgttc
    10021 tgctccccaa ttccgccccg gggcggaatc atatgcgaaa caagaacttc ggccaagagt
    10081 ccccgtgagg aggcctggtg cagcaatgat gacattatgc cgcgtcgcac agcgggcggc
    10141 cgatcggcgg ctccactatg ccggcgtaga atctaggtcc tgtcggcgcg tcgaacattc
    10201 gcatgtcgtt cgcgtcgaac catttgagcc aggcggagag ctcggcgcgc ttgtcgaggg
    10261 cgaattggcc ttcggcatct tggaaacgga gcaaggccgc cggcgccatg gcaatgattg
    10321 catcaatctc atccggcggc agtagccgtt catcgcggat gaagtcagtc atcgcgcctg
    10381 gacggcgttc ggcgccattt cgccccttca ccgatttgcg ccagaggccc tcgacattgc
    10441 gaaggcgcgg tttcgcgcga gcttccatca agacgatccg gcgaagctgg aaaatcggaa
    10501 ggccacgaat ttcccatggc ttggctgcgc cgcacatata gcacgcgctt tttacgggaa
    10561 ggggaagccc ttcggagagg atccgttttt cgcacgccgc ccggtcccat ccccattcgc
    10621 gtagaggata ttcgtattgg tagcgcggat ccaaatggcc ttcagcatgg gcgtaccgtt
    10681 tgctgtcgcg atcgccggcg tcgtagccaa tgcacttcgt cacctttcca ccacgggccc
    10741 accattcgag ggcgggctcc cagctctgca cgaagcgatc ctgaggctcg atcttccatt
    10801 tttgcgaaca gctgtgacgg ccgaaactga tcgacggcag cgtgccgttg gtgaagcagt
    10861 tttcgtcgag tgttcggtac ggaggccagt gcttgaatcg ccgcggctcg tatctgaccg
    10921 tctcgctccg gattccgcgg gcgcgaagcc agcggaatat caaatcgcga tacgcatagg
    10981 tttcgggctt ttctgctcct gtgtcggcga acaggacaag atcgatccgg cgcccctgcg
    11041 cgaccatctc gagcaacatc gcggttgaat cgacgccggc accgaatgcg acgacgacag
    11101 gggcgtcctc gatcggacga tgctggacac agaacatcgt ggtgaccggt gctgagttcg
    11161 ttaggaggcc agcgccgggc tgatctcaag ctgcgcgtcg gccgtcgtga tgaaggcgat
    11221 cgcgttgggc tgcccggcag cgcgctccac ccggtgaggt tgtcgaatgt tgctggtggc
    11281 aacgatcacg aacggcgcgc cgtctatgtc ggaaagcatc acggcgagac ggtacgcatt
    11341 ttcaaaggta gcgagggtcc gcatggcatt gcccttcaag ctggacggat gtggtcgata
    11401 tatggaggac gctcgaagca gttggcgggc gaccatcaat agtcttccgg caacatgatt
    11461 gtgattacgc gcgtcgtgat tgcggcgttt gccggatcct ctgaaccata ctcacaggcg
    11521 aggtcgtaat aatcgatctt cagccagact ttgcggtcct gatagaggat tgaggcgaag
    11581 tcatgctcgg ggctgtcggc tgcgaattcg cagtggcgga aagccttcag caattgagcc
    11641 cggacgatcg cggggtgagt ttccggcgtc gagcggaagg tctcgatgca attgcgggtg
    11701 aacgtgattc gtgcgcggcg atcgaggcca agccgtgcat catcgttcat tcgcgcgatc
    11761 gtcgctgtgc gcgaggtcgt gttttcatcg gtctgcatag ccgtcaccaa tcgtagattg
    11821 gcagctcggc cgaagccgga gcatcgcgat cgaacatgac ccagcagcaa tgacgctggc
    11881 gggcgagcgc tatacagtcg agaaggtccg cagggagttt atcgccgggc tcgccaacat
    11941 agacaaacca gccatattcg cccttttcga agacggcgtg gtcggccttc ttcatccagc
    12001 gattgcaggt ctcgaaggtg agatttccgg tcgacagcac caacatcggc tcgatcatgg
    12061 aaggtctcct gcgcgatgct cgagccacac gcgtagatcg cggcgcatga tgccgatcgg
    12121 cgcgttgatt tcggtgaggg gtgcatccgc cgagatgtaa tttctccctc gaaattcgct
    12181 gacgaagctg cgcatgacgc ggaggtaaag atgcccgccg gcgataccaa tttcggtgaa
    12241 cggacgttcg ccgtggtcgg tcaacgaggc gatctcgcac gtcgcgtaat tgccatagat
    12301 gtcaggcttt cggggtgacg gcggccgcgg ccaccgcatc gcgatcccac cttcgatcat
    12361 acgttccgcg agtgcggtcg tcagccgggt atcgtaattc tcgattggcg atcgggagtc
    12421 gatgaagccg taccgcgcgg agagaacggc gactttcgtg tcctcgcgat cgggaccaat
    12481 cgcgcggagg gtctgccaga gtgggccgtc gtagcgatcg acggcaggaa tccagcctgg
    12541 tcggcttcgt ttcgttgccg aacaagcgag gatgaggagg cggtggcgct gaacattgtc
    12601 catgatgcac ctcatgcggc gagcggcaga ggatccgcgt tcgcggtggc ggcgctgacg
    12661 gcagaatgga tccagtcggt tgcagcttgg gcggcggccg cggcgcggaa gattgcgcga
    12721 gtgtcgtttt cgagcgcgcg gagccagctc gcgacatagg ccgcgtgatc gggccgcggt
    12781 gaataggcga gcccaagatc ggccatgagg aagctggcgc cgatttcggc gacgagctct
    12841 tcccgggcac ggatcgcacg ctccttgtga tagtcgcgca gcgtatcgcg cgcgagcctg
    12901 ctggcgtgac ccgttgcgtg caccagctcg tgaccttcgg tggcgatcat ggcttcatcg
    12961 ctttcgaagg cggagcgtgc cggcatatag atccggtcct cgtcggggcg ataataggca
    13021 tcgtggagac cgaacgtcac cggaacgccg gagtgctcga ggaaagcctt ggcagcggca
    13081 attcgctcgg acatcggcgg gggaacatct tcgtcctcat cgccagcgcc gtcgacctgg
    13141 ctgcgattga agacgacgaa gctgcgagca aagaagcggc cgccccgatc ctggtcgtcg
    13201 gcggtagctg tttcgtcccg ctcctggcgc ttccacagga tgacgtgggt cccggtttca
    13261 cccttgcgga cccgggcatc gagcgacttc cactgttgga aggtcgccca cgttccggac
    13321 tcgaagccgc gcgtttcagc cgaagcccat aatacgaggc gattgattcc gctgtaggcg
    13381 cgtcccgctg cgcttcgggg cctcatgacc ggcgcgccgc ggtgatgcca tggcattcgc
    13441 cactcaccgg cgccggcctc gatcgcggcg acgagctggg ccgtgatgct gctgtagata
    13501 tcgtgacggg gtttcattgg gcggatcctt tctcaagctg cgagcaatgc tcgcttcccc
    13561 ctcccccgct ttcctcctgt cccgcgcttg cgcgggtgat gttcgaaatc gttggtccga
    13621 cgtcggcgag gatatccagc ggaggaatct cgaagtgggc cgcgtcgaac catgcgcgca
    13681 gcgtggcgag gttctcatgc agatcgaggg tgtcgcgggt ggctatggcg ttgccacgtt
    13741 cagttgccgg gtaggtctcg atcgctcccg ttccggtgaa gatgacgcgt tcgggttccc
    13801 agcctcccgg ataaccggca caccaacgca cgaagggaag gccgcgttgg acgcaccaag
    13861 cttcaagggc ttcgaactgg ccgccgatcg cctggtggtc gaaaagctgc agcggttcgc
    13921 cgtcggtcca gtctgcgggc gagaagggat cgccatcctc ttcaacagat aaattctcgg
    13981 cggcaatgag gcggcagagt gtgatgaaat cggctccggt aacggacccg ccgattgtga
    14041 tggacgcggc aacgcgatca gccatcttat tgactccttt aatgaaattt cagaggatcg
    14101 agagcggcgc ggaggcaggt ttccaactgg aagccgagac ccttgcttcc gcgccgtcgt
    14161 ggagacgtca ggcggcctct gccaaagcgt cttcgtgctg agcgacgtcg ttcactccgg
    14221 ggtcgaggtc gtcggcctgt gcgtcgccgc catcgtcgat cgccggttgt tcttcagcgc
    14281 tatcatcgtc gaccggcggc acttcaggtt cgttcggcgt cactgcggaa cgaaattgca
    14341 ttgtctccgg gatccagctc agcgcccgct ccttcacctc gggcgtggca atcgtttcgc
    14401 cagcgcagag ttttgccgct gcggacgaga gtgccgcctt cttttcgctc gcgtaacggc
    14461 ccggaagctc cggatcgagc ttgccaagga tcgagagaat ctgcggtttg cggattttgt
    14521 cgaagaagga ttccgcgctc ggccgccaat gatcggcggt ctcgatctcg agcagcgccc
    14581 caagctgggt ctggaagctg tttttccggt cgccagtggc caggctcgcc tgaaggctgc
    14641 ggctgacggc aaaggcgagc cacgcggcct tatcggcttc gtcgagttcg cggaacgcca
    14701 agaaggcgtc gaagctgtcg gcgaatccgg cccagtcgca aggaagaccg gaacggatgg
    14761 cctcaaggct gtcttgtgca gcggttggct gcgcagcggc gaatcccgtg gggtcgaaca
    14821 gttccccgat ggtcaccatg acgcctgtgt cgttgtaagc ggcgtgcccc gtaaatttcc
    14881 gcgccaggcg aaagatggtg aggtcgagtg cgaggccggg atcttgggcg acgtgcagcg
    14941 ccaagacatc gcgacgatcc ttggcgagct gctcttcgag cgaccgggga aagacgtttt
    15001 cgattgccgc cggttcggcg ccgtcctcgg taacagtggc ggcgcgcttt tcgcgtttcg
    15061 gcgctgccgt tgaaaagtag cggtgcgaaa ccttcggcgt cccgtcgctg gcgaggacga
    15121 tgaacgtgcc gacattgggc cgttcgtctt ccgggatgac gtagctgcgt ttgtcgatct
    15181 cggtatgctc ggcatcgagc gcttcattct cggcctcgag gcgcaatacg acatcctgat
    15241 cggttgcgtc ctcgatctct tcctcgatcg cttgcagacg ttcttcgatc gccgtcatgc
    15301 gggcttgatc cgcatcgctg agttcaccgc gccgaatata ataatcgtgg aggccatcgg
    15361 tctcgttgtg gggaacatgc tgggcgagga cgacacgaac ccatccaagg gtgccgagtt
    15421 gcatgatatc atctgcagct tcttggagct tcgcgcgcgc gatatcttca gcgattccac
    15481 gatcaagcca ttggccttct ccctcgccgg cgaaaagatc gcgttcaacg cgcccaccgg
    15541 ctgccagata ctcctgctca cccacgagct tcgccacggc gctggtcgct tcgatcgcgc
    15601 tttcggtcac gtagcgccgg atcgtatgcg gttgatcgcc ctgccagtgg tctttaaaat
    15661 tgttccaggc cgcaagctgc gtgtcgcgat cggacgtgct gccatatgct tttgcgacat
    15721 cgagggtgat cttgccttcc gcaagcgcaa tgaagatcgg ctctgcgagg tcggcgagac
    15781 gaaggcgccc ctgaacgtga cgaactgtga caccgaaacg gcgcgcaacg tcttcttcgt
    15841 ttgcgccctt gtccatgatg gcgcggtagc cacggcattc gtcggcgggc gacatagctt
    15901 cgcggatgag attctcagcc aaactgattt cttcagcgag cgcggccttt tcgtcattgc
    15961 acacacgggc gtcgaccatg gcattctttg gcagttggcc actttcgatg agctttgcga
    16021 tagctcgcca gcgtcggccg ccagcgtgga catcgaagac tgatttcttg tcggttgctg
    16081 ggctgacgat gatgggctgc agaaggccat atgcgtcgat cgaaaccgct agcgcgtcga
    16141 caccagcttg ggagaacgat tttcgcacat tggtcggcga gaggcggagt ttgttgaagg
    16201 gaatttgctg gttctgcatg atgaatctcc ggtaactacc gagacccgtt tcatgggctt
    16261 aactcccagg gggcctcgcc ccctctcttc cccctcccct tcaagctttg gcgcgccgac
    16321 ggtgcgccag gttggtcgcg gcacgcgaga tattgcgcgc ggccgctatg atcctatggc
    16381 ggatccgtgg ctggaagctt tgaaacactc gaagagatca ggcgaaaacg ctcgaacgtg
    16441 gctgttcggt gcggattctg cgatcatcgc ggtgttgttg acggcggcgt tctatggcgc
    16501 tggttcacgc tgcaccgttg ggacagccgt ccagcggatg tcatggagca tatgcgctgc
    16561 agcgtgtgcc gccgccgacc cgtcgagctc gaggcgacga cggatttgcc aacgatcgaa
    16621 tttgggccgc gcgatgaggc cgaatggcag gaagttgtgg cgaggcagcg ccatcggccg
    16681 cctcgccaac cggattggtg atcaggtgcg tttagccgcg ggtttagccg ctgcgcgcgc
    16741 gggcttgcgg ccgccgccgc gtcccttggt gccaaggccg atttccttgg caagcacccg
    16801 ccgccgctct gcatagtcag gcgccaccat gggataatcg gcgggcaatc cccatttaga
    16861 gcggtagtcg tcaggggtta gcccatagtg cgtcatcagg tgtcggcgaa gcatcttcaa
    16921 cttcttgccg tcttccaagc acacgatgta gtcgggttta accgatgaac ggatcgaaac
    16981 ggcgggaacg ggtttttctt ctggtacttc ggcgggctgt gaaaggcccg acagcgctgc
    17041 atgaacattg ctgatgagga tggagatgtc tgaaatcgcc acgctgttat tgccgacgtg
    17101 cgcagcaaca atatcggctg ttagagtcag aaggtcggaa tcgtgttggg gagtctcggt
    17161 catttaatag tgatcctaat cttagaaatg tgccccgccc gcattcaatc cctattgatc
    17221 gcgcgccacg caagcatttt cttcattccg agctggccgt caaaattcag gttctgccgc
    17281 ttgttctaat cccaattccc cgcagactaa tccgccccgg cgcactgagt ttgcgctaag
    17341 cgcagacttt cttcaggcgt atactctgtc aaaacctctt cgctgatcat cgcttgtttc
    17401 gctcggacgc acgtctggta gggcgagtag tgcatgatga tccacgtgac gcctagagct
    17461 gcgagagcgg caatcagcgc gatcttgacc ttatcgttca tgcttgctca tccctcgcct
    17521 tcgctgcgtt cttacatgtc agagtgcgaa atagcattat ctgcgtaggg cacctacgcc
    17581 tgtcgtcgat ggcggagagg tcatttcccg tcattgcgcg atttccgcga ggatctctgt
    17641 cgcacgagaa acgggaacaa aaagcctcgt tcgaaattgg attatctcag taaaacagcc
    17701 cttcgctttt agtgtcggca gtgatcgagg atcgaatccg ctgagctcga ggcgaggttc
    17761 gccgtttacg cggcggcgtt caagcgtgag ccctaagttg ccaccgacca ttacggtttt
    17821 gccttcccat gcggcggcga caatttcggc gggcgccagc ttgatcgcgc ctgctatgcc
    17881 gagtttgccg agcagcgtgt tcacatcgcc gtgcggaaca atgcggccga gcagcgcacg
    17941 gccatcagcc gtcacgaggc gacgtacttc cggatcgtcg ccgagaaggt tccagatagg
    18001 gagtagacga cccgtcgcga ggaagaagcg ctcgcggttc gggttcagcc tgagctcgtc
    18061 gcactcggcc gtccaacatt gcccgaaggt atcgacgtcg acgcgctccc acatgctctc
    18121 ctcgagatcg ccggcggtga tatattgatg gtgaccagga cggctgagct gccacgttgc
    18181 taccgcctct ccggtgtcag tcaaccgcgt ccgcgccggc gtctgcagtg caactttgcc
    18241 ggaacgtgca tttcttaggc ggattgccct gctgcggaag cgatctgcca atgcagaaac
    18301 ctcggccagc gagcacatct cgcgcttcca ttcggcttcg atagtgagca gtcgcgtgga
    18361 ggaggacgta tcgacacctt cgcgaagcgt aatctcgtcg acgatcgtga gtttgtcggc
    18421 gcggatcgtc tcgacgccgg catcgaatgt gcctgccttc ttcgcggcat cgatgcgagt
    18481 ttcgacaaga cccagatact cgtcgaagat cgcattctga atggcgatcg gcagggcgag
    18541 gatgcggttg agccatcgct ggatcggcgg cagcttatcg accagctcac cttcatattc
    18601 gagacgaagc cctgttcggt ggcagaagtc actgaaagtg acgctggtga gtttgccgcg
    18661 gtgcagaagc cgataccagg tcaacagcgc atccttggca tagtcgcttt cgaggttgtc
    18721 catcgggtcg aagagatttt gcccgccggt ctgacgttgg ccccgcgtaa gggcgccaag
    18781 ggcgtcgagc cggcgcgcga tcgtcgagat gaaccggcgt tcgccgcggc agtctgtagt
    18841 gaccggacga aagatgggcg gctgcgcctg gttggtccga ttggtgcgac cgagcccctg
    18901 cacggcccga tcggcgcgcc agcccggctc aagcagaaaa tgaacgcggc gctgctgatt
    18961 ctggtgatca aggctcgcat gataggatcg gcccgttccg ccagcgtcgg aaaagacgag
    19021 aatgcgcttg gcgccgttca tgaatgcgtc agcgtctgcc atgcaagccg atggcgatcg
    19081 cgactgcagc ttctggctac catctggaag acggacgagg cgcttggtgc gtccggtgac
    19141 ttcggcaacc gcgtcggtac caaaccgttc gatgacggca tcgagggcgg gcgcgatcgg
    19201 cggcatggcc ccgagcagct cgagtgtgtc ggcgcggatt tgcaatgcct cctcgctgtg
    19261 aaccggattg ccgtcgacgt cggagcgtgg tcgcgagcgt tcattgccgt cgtcatcgct
    19321 gatgatctcc atcgcccggg tcggaaacgc cgcttcgagg taatccatga cgagctcgcg
    19381 aggcgagaga tccaacgcca gttctgcgcg atcggcggga tcgagatcgg ccaggcggcg
    19441 cgagagcagg gcttcgctgg tggtgacgag ctggatgacg gcgctctgct cggcctgaag
    19501 agccgcttcg atcgccggca gaaggcttgg cagtttcatc gcgaggagaa gtgctgagaa
    19561 gaatcgctgt ttggcgcttt cgaagcgcga tcgcgctgca gagagggctt ggccgttaag
    19621 cgtcgcgccg gtcagccgat cgacgacgcc ggcggccgca agcgcttctt ccatattgcg
    19681 gtggatgatc gcccatgcgt cggcataggt gtcgaagtcc acgatctggg tgggcgtgag
    19741 ctcatgttcg aggatgtcat attcgacgcc ggcataggac agcgcacggg cggtatagac
    19801 acccatcgcc ttcagctcgc gcgcgaccag ctccatcgct gcgacgccgc catcgcggat
    19861 ccgtgcggtg aaggcttcgc ggtcgacgaa ggcagtgccc tcaccccaaa ggccaagccg
    19921 ggtcgcatag gccagattgt tgacgtcgga tgcgccagtg gcggacgcat aaatgacacg
    19981 ggcccgcggc aaccggtttt ggagctcaac gcctgcaagt ccctgctgtg agcccgaaat
    20041 tgtaccgaag cgcccttccc cgccagcaac gccgcccatg gcgtgcgctt cgtcgaagag
    20101 gatcaagccg tcgaaatctt cgctgagcca ggcgaggatc tgatcgacgc gccgatcgcc
    20161 accagcttcg ctccgtaggg tggcgtagga cgcaaacaat atggaatcgc cgatctcgat
    20221 gccggcgccg gggcgaacgc gaccgagtgg ttggatgtcg agaggtgtgc cgcctagagc
    20281 ctgccagtct cgtcgtgcat cttcaatcag cgcgctgcta tcactcagcc agatatgacg
    20341 ccgtttgccg cgcagccact gatccattgc tatcccggcg agctggcgcc ccttccctgc
    20401 tccggtgccg tctccaagga agaagccctg ccgatagcaa ttgccatcgg cgtgcggaac
    20461 gagcgcaata cccttctccg gcaagcggta acggcccggg agatcgattt ccgtcgctgc
    20521 aagcgcatgt acgatcgtct cgagctgggc agcgctcaat atcttgtcgt cgaggacgcg
    20581 cttaggaagc aacgggcgat agctcggtgc cggcagtgcg acggccgcca ttgcggcgga
    20641 ttcgaccagc gggctcggat gttcagagga atgctcgaat tcgagacgct gactgcgcca
    20701 cggcacatag atcgcggcct cgtcgtgcgc cgcggcgacg ggatcatgga cgcgatagtt
    20761 gagggtggcg atttcgaagc tcggtagcgg cctcgccgga cggggaaatg atttggcggg
    20821 ggccgttcgg aaagcaccaa atagagcgac tggctttgtt gtccgcgggc gtaccggagg
    20881 ttcggggtct ggctgaagtt ttgcccgcgg cgggatcgcg cgaattgcct cgagaagctt
    20941 gctggccgat gcgcggttta tcgtcgacgg cgggcgtttc cctgtggttt ctttgtcggc
    21001 gacaatgatc cgaactgcga ccgacgtccc cttccctgca aaacctttgt cgaggcggag
    21061 cattaggcga acggttgtgc cggaaactgc gcggtcaaat agtgacccgt gtttaccgcc
    21121 cgggttgaag ctgtcgggca tgatcgcgac gatacggccg ccgtgctgca gggccgcgag
    21181 tgcagctacg aaatgacgat gcgccgcgaa aggatcctcg gcgccggaag cgttgcgggc
    21241 gaacggcgga ttcatgatca cgacggtcgg ccgcacggaa acatgctggt tgatctgcgc
    21301 ggcgttgaag gtcgtgacgc tgcattcggg gaagagattt cgaagaactg cggctcggac
    21361 ctgatccagt tcattgagct ggagcgtctt cccctgcccc gcccattggg cgagtgtccc
    21421 aatgccggcg ctcggttcga gaaccacgtc ttccggttca ataatggcaa ggtggtttac
    21481 cagccaagcg agatccaacg gggtagagaa atgctgatga gcgatttgct cttcgctgcg
    21541 caccgtttgc gtcgggaagg ctttttcgag tgtcgcgagc tggttgatga tagccaatgg
    21601 ctgatccggc aattttacgc ttcgcgctga gaggatcgcg gatatttcgg tcatcacgaa
    21661 actatcacgt tgggtccaag cgccttcggc tgatttagcg ccgaacgccg cggtcattgc
    21721 ctcattgata agccctcttc ggactgcctt gtcgtcatcg aactgcgtgg cgatcgagcg
    21781 tgcggcatca atcaggcgcg ccgcggtgtc gggggcctgg tccgtatcgg tgagcatggg
    21841 aggatccttt cctacggacc gctatggtcc ggaaagtctc ccccctcttt tctcctccaa
    21901 aaaacgccca agcgcagcgc gggaggcttc taagattcaa gtttctaaga ttcgggcaca
    21961 aaaagtgttt tagactcagt gccttgcatg ccctttatat gagggtttgg gggcgtcttt
    22021 gcgaggtcat gggggctttc atatgaggtg ccgggggcct gcgggtgagc gttaggggga
    22081 ttatgtgagg aatcgggggg cgctgtgaat gcgtcggaat ttgcaacccg atcgctttga
    22141 ctctgcgcgt ttttagaatg ctgagcgcct gttgatgtct gaaatgcgat gttgcgcgag
    22201 ggctgagggc tttcgaactg cttccggtgt caattatgtg agcgttcggg ggcagtgatg
    22261 ggttgcgcgt tgctcgtttt gtatgaggaa ccgggggcac gtgttcgttt atgtgaggat
    22321 ttgggggact ctccatttgg ggcggttttc tgcggatgtt tgccccccat tatatgagtt
    22381 cgtgggggct tcgagttttg gggtggcaca gacgaagtga gagacccggc aaccacggag
    22441 gtcgcgttag tatgagtgat cggggggcta ttctctttat gaca
//
LOCUS       DATMSU010000280         4588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_23716, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000280 DATMSU010000000
VERSION     DATMSU010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23716"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VN034_07650"
     CDS             <1..339
                     /locus_tag="VN034_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate-protein ligase B"
                     /protein_id="HWV60510.1"
                     /translation="ALEGWVIDALALLGVEARRAEGRIGIWTDDADGREAKIGAIGVR
                     VKRWVTMHGFSLNVAPDLAHFGGIVPCGIAEFPVTSLAALGKAATFADVDAALARTLP
                     AFLDKLGPTD"
     gene            350..688
                     /locus_tag="VN034_07655"
     CDS             350..688
                     /locus_tag="VN034_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60511.1"
                     /translation="MTRIILASLPLLALAACGGGDSKGKSTTTLDAVEVQPGSVSDSM
                     IILDNVAGDGTAVDNSIPEDETKKDAPKAEEASEGDEAASDDGDDPEAVDMPAAKKAI
                     TDDNAAKKGE"
     gene            complement(692..1324)
                     /gene="queE"
                     /locus_tag="VN034_07660"
     CDS             complement(692..1324)
                     /gene="queE"
                     /locus_tag="VN034_07660"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HWV60512.1"
                     /translation="MVYAVKEIFLTLQGEGAQAGRRAVFCRFAGCNLWTGREQDRAKA
                     VCQFCDTDFVGTDGTLGGKYRSAAALAEVIADSWGEGGDDRYVVLTGGEPMLQVDDAL
                     IGALHDRGFTIAIESNGTLPIPRSIDWICVSPKAGSELVQTSGDELKLVWPQPGSDVA
                     RLAALDFKHLLVQPLDDPNASANVQACIDLVMDDPRWRLSLQTHKHLGLR"
     gene            complement(1329..2027)
                     /gene="queC"
                     /locus_tag="VN034_07665"
     CDS             complement(1329..2027)
                     /gene="queC"
                     /locus_tag="VN034_07665"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWV60513.1"
                     /translation="MQQVSGKSVIVLLSGGLDSMVCAGLAREAGARIVALTIDYNQRH
                     RVELESAARIAEYVGAAEHIVLPLDLRRFGGSALTADIDVPKNGVPEVDGGDDVPITY
                     VPARNLIFLSLTLGLAEARAAQDIVIGVNALDYSGYPDCRPEFIQGFEKLAALATRDG
                     DKGVEFRIHAPLQHMTKADIAAEAARLGMDAGMSWSCYDPTPEGLHCGGCDSCRLRSK
                     GFADAGLADPTRYA"
     gene            complement(2312..2683)
                     /locus_tag="VN034_07670"
     CDS             complement(2312..2683)
                     /locus_tag="VN034_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60514.1"
                     /translation="MALGLAGAATAVPAQAPSLAMLDRLEKGSWQLRERGKDAVVRTV
                     CVGDARRMIQVYHPGATCSRYVIEDTPNSVTVHYTCPGAGHGRTTIRSETNRLVQIDT
                     QGIAEGRPFSQAIEARRTGGC"
     gene            complement(2849..3772)
                     /locus_tag="VN034_07675"
     CDS             complement(2849..3772)
                     /locus_tag="VN034_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HWV60515.1"
                     /translation="MSETIETWFDQPLLFTIAERHVRGRLVRLGPTLRTIIGAHNYPP
                     VAEKLLAEALVLTVLLGSTLKDENGQMTLQAQTGGGPVELLVCDYRGGELRGYLKFDA
                     ERLAEAGSDPTLFALFGEGFLAITFDQATTGERYQGIVPLEGASIGEAAEHYFEQSEQ
                     ISSLIKLAARHDPEAGCLAGGLLLQHLPEGEVGRDRLHVRHDNPEWEHAKTIAATLKE
                     EELTDPATSLETLAWRLFHEDEVRVEPGVTVSRGCRCSTGYFAGVLAQFPEAERAEMA
                     DDDGRIVVDCAFCSRFFPIPLDEIATHDRGE"
     gene            complement(3783..>4588)
                     /gene="argF"
                     /locus_tag="VN034_07680"
     CDS             complement(3783..>4588)
                     /gene="argF"
                     /locus_tag="VN034_07680"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWV60516.1"
                     /translation="APLAGHVLAMVFEKNSTRTRASFDIAIRQLGGVPMIMDAGVTQL
                     GRGETIADTARVLSRYADAIMIRTDDHAKVEELAEYASVPVINGLTDLSHPCQIVADL
                     LTIVESGKALPGLELAWLGDGNNVLASLIEAAGLFGFHVRAGVPQGYEPEASFVEAAR
                     AKGSRVEIIRDAREAVAGADLVVTDTWVSMGQDHAHNKIAAMAPYQVDERLMAGAKGD
                     AIFLHCLPAHRGDEVVDAVIDGAQSRVWDEAENRVHAQKSILLWALGKL"
BASE COUNT          779 a         1554 c         1508 g          747 t
ORIGIN      
        1 gcgctcgaag gctgggtgat cgacgcgctc gcgctgctcg gcgtcgaagc gcggcgggcg
       61 gagggacgga tcggcatctg gaccgacgat gccgacgggc gcgaggcgaa gatcggcgcg
      121 atcggggtgc gcgtgaagcg ctgggtgacg atgcacggct tttcgctcaa cgtcgcgccc
      181 gacctcgcgc atttcggcgg gatcgtccca tgcggcatcg ccgaatttcc ggtgaccagc
      241 ctcgccgcgc tcggcaaggc cgcgaccttc gccgacgtcg acgcggcgct ggcccggaca
      301 ctgcccgcct ttctcgacaa gctcgggccg accgattagg aaccctgcca tgacccgcat
      361 cattctcgcc tcgcttcccc tcctcgccct cgccgcctgc ggcggcggcg acagcaaggg
      421 caaatcgacg accacgctcg atgcggtcga ggttcagccc ggatcggtga gcgattcgat
      481 gatcatcctc gataatgtgg cgggcgacgg caccgcggtc gacaacagca tccccgagga
      541 cgaaacgaag aaggacgcgc ccaaggccga agaggcgagc gagggcgacg aagccgcgag
      601 cgatgacggc gacgatcccg aagcggtcga catgccggcc gcgaaaaagg cgatcaccga
      661 cgacaacgcc gccaagaagg gcgaatagaa gctaacgcaa tcccagatgc ttgtgcgtct
      721 ggagcgacag gcgccagcgc gggtcgtcca tcaccagatc gatgcaggct tgcacattgg
      781 cgctcgcgtt cgggtcgtcg agcggctgga cgagcagatg tttgaagtcg agcgccgcga
      841 gccgcgcgac atcgctgccg ggttgcggcc agacgagctt cagttcgtcg ccgctcgtct
      901 ggaccagttc gcttcccgct ttcggactga cgcagatcca gtcgatgctg cgcgggatcg
      961 gaagcgtacc gttgctttcg atcgcgatgg tgaagccgcg atcgtgcagc gcgccgatca
     1021 gtgcatcgtc gacctgcagc atcggctcac cgccggtgag cacgacatag cggtcgtcgc
     1081 cgccctcgcc ccagctgtca gcgatgacct ccgccagcgc cgcggcggat ctatatttgc
     1141 cgcccagcgt cccatcggtg ccgacgaaat cggtgtcgca gaactggcag accgccttgg
     1201 cgcggtcctg ctcgcgtccc gtccacaaat tgcacccggc aaagcggcag aaaaccgcgc
     1261 ggcggcccgc ctgcgcgccc tccccttgca aggtgaggaa aatctctttg accgcgtaga
     1321 ccatcggctc aggcgtaacg cgtcgggtcg gcgagcccgg cgtcggcgaa gcccttcgat
     1381 cgcaagcggc agctgtcgca cccaccgcag tgcagtcctt cgggcgtcgg gtcatagcac
     1441 gaccaactca tccccgcgtc catgccgagc cgtgccgcct ctgcggcgat gtcggccttg
     1501 gtcatatgct ggagcggcgc gtggatgcgg aattcgacgc ccttgtcgcc gtcgcgcgtc
     1561 gcgagcgcag cgagcttttc gaagccctgg atgaattcgg ggcggcagtc gggatagccc
     1621 gaatagtcga gcgcattgac cccgatgacg atgtcctgcg ccgcccgtgc ctcggcgagc
     1681 ccgagcgtca gcgacaggaa gatgaggttg cgcgcgggga cataggtaat cggcacatcg
     1741 tcgcccccgt ccacttcggg caccccgttc ttgggcacgt cgatgtcggc ggtaagcgcc
     1801 gagccgccga agcggcgaag gtcgagcggc aggacgatat gttcggcggc gccgacatat
     1861 tcggcgatgc gcgcggcgga ttcgagttcg acgcggtggc gctggttata gtcgatcgtc
     1921 agcgcgacga tgcgcgcgcc cgcttcgcgc gcgaggcccg cgcagaccat cgagtcgagc
     1981 ccgcccgaca ggaggacgat cacgcttttt ccggaaactt gctgcatcgg cgccagatag
     2041 gcgcacgata atcatcgcgc aactcccctc ccgcctgcgg gaggggcagc gagacttgcg
     2101 agcttgctcg ctagtcgcag cggggtgggc ggccgcagcg ccgcaggccc acccctaacc
     2161 cctcccgcaa gcgggagggg aacttaaaaa gaaacgggcc gcgaggcggt tgccacgcgg
     2221 cccagtccgg catgacagcc gttagggaga atggcacaca tcgatatgcc atggctccgc
     2281 tctaaccgcc gatggttaat atttcgttcg cttagcagcc gcccgtgcgc cgcgcctcga
     2341 tcgcctgcga aaagggccgc ccctcggcga tgccctgcgt gtcgatctgg accagccggt
     2401 tcgtctcgct gcggatcgtc gtgcggccgt gacccgcgcc cgggcaggta tagtggacgg
     2461 tcaccgaatt cggcgtgtcc tcgatgacat agcgcgaaca ggtcgcgccc gggtgataga
     2521 cctggatcat ccgccgtgcg tcgccgacgc agaccgtgcg gacgaccgca tccttgccgc
     2581 gttcgcgcag ctgccagctg cctttttcga gccggtcgag catcgccagc gacggcgcct
     2641 gcgccggcac cgcggttgcc gcgcccgcaa ggccgagcgc catcaaagaa tatcgcacga
     2701 agggagagaa gatcgccatt ttttcctgtc cactggccgc ccgtgtgcgg gctggaatgc
     2761 gaccccgtta cctatagcta acaacgcggc ggccgatttt caaccgcttg gcgctaaatt
     2821 cgctcgcttc ggcgccgaaa cggcctgact attcgccgcg atcgtgggtc gcgatctcgt
     2881 cgagcgggat cggaaagaag cgcgagcaaa aggcgcagtc gacgacgatc cggccgtcgt
     2941 cgtcggccat ttcagcgcgc tcggcctcgg gaaattgcgc gagcacgccg gcgaaatagc
     3001 cggtgctgca ccggcacccg cgcgacaccg tcacgcccgg ctcgacgcgc acctcatcct
     3061 cgtggaaaag ccgccaagcc agcgtctcga gcgaggtcgc gggatcggtg agttcctctt
     3121 ccttcagcgt cgccgcgatc gtcttggcat gctcccattc gggattgtcg tggcgaacgt
     3181 gcaaccggtc gcgaccgacc tcgccctcgg gcagatgctg gagcagcagt ccgccggcga
     3241 ggcagcccgc ctcgggatcg tggcgcgcgg cgagcttgat caggctcgaa atctgctccg
     3301 actgctcgaa ataatgctcg gcggcctcgc cgatcgaggc gccttcgagc gggacgatgc
     3361 cctgatagcg ttcgccggtc gtggcttggt cgaaggtgat ggcaaggaag ccttcgccga
     3421 acagcgcgaa caaggtcggg tccgacccgg cctcggctag ccgttcggcg tcgaatttga
     3481 ggtagccgcg cagctcgccg ccgcgataat cgcagacgag cagttcgacc ggcccgccgc
     3541 cggtctgcgc ctgcagcgtc atctggccat tctcgtcctt gagcgtcgag ccgagcagca
     3601 cggtcagcac cagcgcctcg gcgagcagct tttcggcgac cggcggataa ttatgcgcgc
     3661 cgatgatcgt ccgcagcgtc ggcccgagcc gcaccagccg cccgcgcaca tggcgctcgg
     3721 cgatggtgaa cagcaggggc tgatcaaacc aggtttcgat ggtctcgctc acgtccttaa
     3781 actcacaatt ttccgagcgc ccagagcagg atcgactttt gcgcatggac gcggttttcg
     3841 gcctcgtccc acacgcgcga ctgcgcgccg tcgatcaccg catcgacgac ctcgtcgccg
     3901 cggtgcgcgg gaaggcaatg gaggaagatc gcgtcgccct tggcgcccgc catcagccgc
     3961 tcgtcgacct gatagggcgc catcgcggcg atcttgttgt gcgcatgatc ctggcccatc
     4021 gacacccagg tgtcggtgac gaccagatcg gcgcccgcga cggcttcgcg cgcgtcgcgt
     4081 atgatctcga cgcgcgatcc cttggcgcgc gcggcctcga cgaagctcgc ctcgggctca
     4141 tagccctgtg gcacgcccgc gcggacatga aagccgaaca gccccgccgc ctcgatcagc
     4201 gacgcgagca cgttgttgcc gtcgccgagc cacgcgagtt cgagccccgg cagcgccttg
     4261 ccgctttcga cgatcgtcaa aaggtcggcg acgatctggc acgggtgcga cagatcggtc
     4321 agaccgttga tcaccggcac gctcgcatat tcggcgagtt cctcgacctt cgcatgatcg
     4381 tcggtgcgga tcatgatcgc atcggcatag cgcgacagca cgcgcgcggt atcggcgatc
     4441 gtctcgccgc gcccgagctg ggtcacgccc gcgtccatga tcatcggcac cccgccgagc
     4501 tggcggatcg cgatgtcgaa cgaggcgcgg gtgcgcgtcg aattcttctc gaacaccatc
     4561 gcgagcacat gccccgcgag cggtgcgt
//
LOCUS       DATMSU010000281         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_137579,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000281 DATMSU010000000
VERSION     DATMSU010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137579"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1330
                     /locus_tag="VN034_07685"
     CDS             29..1330
                     /locus_tag="VN034_07685"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWV60517.1"
                     /translation="MARAWAAGYRAAFTCSSLWNGAGKSLAAIERDELTNIYPEIEAD
                     VRALKAEVDEAAKRVSVTWRDDMPPRIAEWSGREGCVTLPIGAKPGPASQPPERRPDL
                     DARPWPMGDKGARWPVPKGQTKFDLATADMAGFGGRTSSLLIVKNGRIALERYWGGHD
                     IHTAQRTFSVAKSMAVTLIGHAAQQGRIDVTQPAQIAEWQTPGDPRAAITTEQLMRMA
                     SGLTSDTAGNRTDAIYMGGASVRQWTTQWPLLHPPGTRYRYANNDTLLATLSLKTALE
                     KTGTPLDPAAFFDRLGMTRTFAEHDKDGNYILSSQVWTSARDLARLGLLYQNDGVWQG
                     KRLLPEGWRRFVTTPSGPQPDRDFGYGAGFWLLAKSDGIPADAFGGFGNRGQYLVIIP
                     SRALVIVRQGYDDGKVRLDIAKLVAAVVAADDNDRRYSKGG"
     gene            complement(1312..1728)
                     /locus_tag="VN034_07690"
     CDS             complement(1312..1728)
                     /locus_tag="VN034_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV60518.1"
                     /translation="MTDEAPRASGQCHCGEIRYSMSTAVQHHALCHCSDCRRHSGAPL
                     VGWALVGNDEIEISGTAKIYASSEHGRRHFCGNCGTGLFYTNEAIFPGQIDVQSATLD
                     DPDQIPAQAQIQTAERIGWMEKLGDLPAFERYPPFE"
     gene            1844..3550
                     /locus_tag="VN034_07695"
     CDS             1844..3550
                     /locus_tag="VN034_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60519.1"
                     /translation="MTARKEVFSLTVRRPALLAVMAFAIAALQGPTTSLTLFYLRWQD
                     RWLLLAAVLLLALCFWRLRAHDRPLRADWRLASVVGATMAVAAFAGHYLILQGYDLSR
                     DEQMATFDSAVFAQGRLVAPLTGIWQDHAEALNTMFMYPTGKAAAWISAYLPLNAALR
                     ALFGLLGDAALTGPAMVLIGALALWGCVRRIWPDDGQAAAVALLLYLGSGQILFNGMT
                     AYAMPAHLALNLCWLWCFLRRTIWSDAAALAVGFVAVGLHQPIMHPMFAAPILFLLVL
                     ERDWRRAGLYLFGYAAIGAFWAWWPNAMWPLVEADLLAPKSDGIDYLSRLAMTVAQGD
                     GVRVANMVSNILRFFAWQHVLLLPLVALGVVLVRRNRLACALAGGIALTTAVMFIILP
                     YQGHGFGYRYFHGLIGNFILLAVYGWTSLGAAQAFWRGLLLRTTVVALILIQPLQAWM
                     AYSFYAAPARASERIANSGADYAVIGESDAPFIVDLVINPPALDQRPIRLLRGQIDPG
                     LAKALCTARPAPLVALVGKDLIRPIRSYYGFEGREANADEANRAVAARLRQFGCRTIL
                     IG"
     gene            complement(3551..4528)
                     /locus_tag="VN034_07700"
     CDS             complement(3551..4528)
                     /locus_tag="VN034_07700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV60520.1"
                     /translation="MPASPARLVGLRCVNPTERLTGLPMPIPGPRSILRLRRAIREAD
                     AVVIHDALYATSILAMLFAKMAAKPVVMVQHIGVIPFANPVMDGIMRLANFVVTRPMM
                     AATDRLVFISATVRDDLLGIPPRQQSLLVFNGVDQSIFNARSRAPASETRARWGLSET
                     APLAVFVGRFVEKKGLRILRLLAEKRPDVQFALLGRGSIDPSEWNLANVHVLGQQPQA
                     VIADIYRAADMLLLPSVGEGYPLVVQEAMACGLPVICGDGSARADPAASRWLKGVNID
                     LADPHGSAAHCAAAIDALATPGESAAMAQYAADTYSWRRMAAALLDGLH"
BASE COUNT          741 a         1429 c         1534 g          842 t
ORIGIN      
        1 ggcgcaagcg cccaagcccg atgccgaact ggcccgcgcc tgggcggcgg gctatcgcgc
       61 cgccttcacc tgctcgtcgc tgtggaacgg tgcgggcaag tcgctcgcgg cgatcgagcg
      121 cgacgagctc accaacatct atcccgagat cgaggccgac gtccgcgcgc tcaaggcgga
      181 ggtcgatgaa gcagcgaaac gcgtcagcgt gacctggcgc gacgatatgc cgccgcggat
      241 cgccgaatgg agcgggcgcg agggctgcgt cacgctgccg atcggggcga agccagggcc
      301 ggcaagccag ccgcccgaac ggcgccccga tctcgatgcc cggccttggc cgatgggcga
      361 caagggcgcg cgctggccgg tccccaaggg gcagacgaag ttcgatctcg cgacggcgga
      421 tatggccggc ttcggcggcc gcaccagttc gctgctgatc gtcaagaatg gccgcatcgc
      481 gcttgagcgc tattggggcg gccacgatat tcacaccgcg cagcgcacct tttcggtcgc
      541 caagtcgatg gcggtgacgc tcatcgggca tgccgcgcaa caagggcgga tcgacgtgac
      601 gcagcccgcg cagatcgccg agtggcagac gccgggcgac ccgcgcgccg cgatcaccac
      661 cgaacagctg atgcgcatgg cgagcggcct tacgagcgat accgccggca accgcaccga
      721 cgccatctat atgggcggcg cgtcggtgcg ccagtggacg acgcaatggc cgctgctcca
      781 tccgccgggc acgcgctatc gctatgccaa caacgacacg ttgctcgcga cgctgtcgct
      841 gaagaccgcg ctcgaaaaga cgggaacgcc gctcgatccc gccgccttct tcgaccggct
      901 cggcatgacg cgcaccttcg ccgagcacga caaggacggc aattatatcc tgtcgagcca
      961 ggtatggacc agcgcgcgcg acctcgcgcg gctcggcctg ctctatcaga acgacggcgt
     1021 gtggcagggg aaacgcctgc ttcccgaggg ctggcgccgc ttcgtcacga cgcccagcgg
     1081 gccgcagccc gaccgggatt tcggatatgg cgcgggcttc tggctgctcg ccaagtcgga
     1141 cggcatcccc gccgacgcct tcggcggttt cggcaaccgc ggccaatatc tcgtgatcat
     1201 cccgtcgcgc gcgctcgtca tcgttcgcca gggctatgac gacggcaagg tccggctcga
     1261 catcgcgaag ctcgtcgcgg cggtcgtcgc cgccgacgat aacgaccgcc gctattcgaa
     1321 gggcggatag cgttcgaagg cgggcaggtc acccagcttc tccatccagc cgatgcgctc
     1381 ggcggtctgg atttgcgcct gcgcggggat ctggtcggga tcgtcgagcg ttgcgctctg
     1441 cacgtcgatc tgccccggaa agatcgcctc gttggtatag aagagcccgg tgccgcaatt
     1501 gccgcagaaa tggcggcggc catgctccga cgaagcatag attttcgcgg tgccgctgat
     1561 ctcgatctcg tcgttgccga cgagcgccca gccgacgagc ggcgcgcccg aatgacggcg
     1621 gcaatcgctg caatggcaca atgcatggtg ctgcaccgcg gtgctcatcg aatagcggat
     1681 ttcgccgcaa tggcactggc cgctggcgcg gggggcttcg tcggtcatcg ctctctcctt
     1741 cgattgggaa cataacataa acaaagaata aggcaaagtt gtcgtggagg cgattccctg
     1801 ggaggttaac gtccgattat ccatgatatg ttaggccgcc gccatgacgg cgagaaaaga
     1861 agttttcagt ctgacggtgc ggcgaccggc gctccttgcc gtgatggcct ttgcgatcgc
     1921 ggcgttgcag gggccgacaa ccagtttgac cctgttctac cttcgttggc aggatcgatg
     1981 gctgttgctc gcggctgtcc tgctgctcgc cctctgtttt tggcgacttc gcgcgcatga
     2041 ccgcccgttg agggcggatt ggcggttggc atcggtcgtc ggggcaacga tggcggttgc
     2101 cgcattcgcc ggccactatt tgatcctgca gggatatgat ctcagccggg acgaacagat
     2161 ggcgacgttc gattcggctg ttttcgcgca aggacggctc gtcgcgccgc tgaccgggat
     2221 atggcaggac catgccgaag ccttgaacac gatgttcatg tatccgaccg gcaaggcggc
     2281 tgcctggata tcggcctatc tgccgctgaa cgccgcgctg cgggctctgt tcggcctgct
     2341 cggcgacgct gccctgacgg gccccgcaat ggtgctgatc ggcgcgctgg ccctatgggg
     2401 atgcgtgcgc cgcatttggc cggacgacgg tcaggcagcg gccgtggcgc tgctcctcta
     2461 tctcgggtcg ggacagatat tgttcaacgg catgacggca tatgcgatgc cggctcacct
     2521 tgccctgaac ctgtgctggc tctggtgttt tttgcgccgg acgatctgga gcgatgcggc
     2581 ggcgttggcg gtcggcttcg tcgccgtcgg cctgcaccag cccatcatgc acccgatgtt
     2641 cgccgctcca attctgttcc tgctcgtcct ggaacgcgac tggcgacggg ccggcctgta
     2701 tcttttcgga tatgccgcga tcggtgcttt ttgggcgtgg tggcctaacg ccatgtggcc
     2761 cttggtcgag gcggaccttc tggcgcccaa aagcgacggt atcgattatc tcagccggtt
     2821 ggctatgacc gttgcgcagg gcgacggggt gcgcgttgcc aatatggtat ccaacatcct
     2881 gcgctttttc gcgtggcagc atgtcttgct gctgccgctt gttgcgctgg gtgtcgtgct
     2941 ggtgcggcgg aaccggctcg cgtgcgccct ggcgggcggc atcgccctca cgacagcggt
     3001 gatgttcatc atcctgcctt atcagggcca tgggttcggc tatcgctatt ttcacgggct
     3061 gatcggcaat ttcattctgc tcgccgtcta cggatggacg tcgcttggcg ccgcgcaggc
     3121 gttctggcgt ggcctgttgc tgcggaccac cgtggtggcg ctgatcttga tccagccctt
     3181 gcaggcgtgg atggcctatt cgttttacgc cgcccctgcg cgcgcgtcgg aacggatcgc
     3241 caacagcggg gcggattatg cagtgatcgg cgaaagcgat gcgcccttta tcgtcgatct
     3301 ggtcatcaat ccgccggcgc tcgaccaacg gcctatccgg ttgctccgcg gccagatcga
     3361 tcccgggctg gcgaaggcgc tatgcacggc acggccggcg ccgcttgtcg ccttggtcgg
     3421 caaggacctg atccgcccga tccgctccta ttatggcttt gagggccggg aagcgaatgc
     3481 cgatgaggcc aatcgggcgg tcgccgcgcg cctccggcag tttggatgcc ggacgatcct
     3541 tatcgggtaa tcagtggagg ccgtcgagaa gagcggccgc catccggcgc caactataag
     3601 tatctgccgc atattgggcc atcgccgccg attcgcctgg tgtggcaagc gcgtcgatcg
     3661 cggcggcgca atgggcggcc gaaccgtgcg gatcggcgag atcgatgttg acgcctttca
     3721 gccatcggct tgcagcgggg tcggccctgg cgctgccgtc gccgcaaatg accggaaggc
     3781 cgcaagccat cgcttcctgc acgaccaggg gatagccttc gccgacgctt ggaagcaaca
     3841 gcatgtcggc ggcgcgataa atgtcggcga tgaccgcctg gggctgctgg ccgagaacat
     3901 ggacattcgc cagattccat tcggacggat cgattgaacc ccggccgagg agcgcaaatt
     3961 gcacgtcggg tcgtttttcc gccagcaacc gaaggattcg aagccccttc ttctcgacaa
     4021 agcggcccac aaaaactgcg agcggcgccg tttcggatag cccccatcgg gcgcgggtct
     4081 cgctggcggg cgctcgcgac cgggcgttga agatcgactg gtcgacgccg ttgaaaacga
     4141 gcagcgactg ctgccgtggc ggtatgccga gcaggtcatc gcgtaccgtt gcgcttatga
     4201 aaacgaggcg gtcggtggcc gccatcatgg gccgcgtcac gacgaaattc gcgaggcgca
     4261 tgatgccgtc catcacggga ttggcaaagg gaatgacgcc gatgtgctgc accatgacga
     4321 caggcttcgc agccattttt gcgaagagca tcgccaggat cgaggtggca tagagcgcgt
     4381 cgtggatgac gacggcatcg gcttcgcgga tcgcacggcg caggcgtagg atcgagcgcg
     4441 ggccggggat cggcattggc aggccggtaa gcctctcggt cgggttgacg cagcggaggc
     4501 cgacaaggcg ggcagggctg gcggggatcc cgtcggcgtc gctcgc
//
LOCUS       DATMSU010000282         5026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_23719, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000282 DATMSU010000000
VERSION     DATMSU010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23719"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="thiD"
                     /locus_tag="VN034_07705"
     CDS             complement(<1..656)
                     /gene="thiD"
                     /locus_tag="VN034_07705"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWV60521.1"
                     /translation="MTARVLIIAGSDSGGGAGIQADIKAVTMLGGHAMTAITAITAQN
                     TLGVQGVHPVPTDMVLAQIDSVASDIGVDAVKIGMIGSAETATAVAERLSRPDLAQAA
                     LVFDPVMIASSGSVLADEATIAAFKALLDRAMVATPNLPELEALGGEEAVLAHGCSLL
                     VKGGHAEGATVIDRLLETGEGEVARWEAPRIDTPHSHGTGCTLASAIACGLAQGMPLE
                     P"
     gene            complement(763..987)
                     /locus_tag="VN034_07710"
     CDS             complement(763..987)
                     /locus_tag="VN034_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HWV60522.1"
                     /translation="MLEMRPDCEKCGRDLPADLHGAFICSFECTFCANCADKLDERCP
                     NCGGDLLDRPLREGAALAKFPASTERKHKA"
     gene            complement(1021..2358)
                     /gene="glmM"
                     /locus_tag="VN034_07715"
     CDS             complement(1021..2358)
                     /gene="glmM"
                     /locus_tag="VN034_07715"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWV60523.1"
                     /translation="MRKFFGTDGIRGLTNQVPMTAEVAMRVGMAAGAHFLRGTHKHRV
                     VIGKDTRLSGYMLENALVAGFTSVGMDVVQVGPMPTPAIAMLTRSMRADLGVMISASH
                     NPFRDNGIKLFGPDGYKLSDEDEAQIEHLLTVEPKLADAAHIGRAKRIDDARGRYIHA
                     VKQSLPESVRLDGLRIVLDCANGAAYNSAPTVFWELGADVVAIGVEPNGTNINDKCGS
                     TAPALLQETVVASGADIGVALDGDADRLIVVDEKGRIIDGDQIMGLIGASWARQGRLK
                     GGGVVATVMSNLGLERFLEGEGLRLERTKVGDRYVLERMKEGGFNVGGEQSGHMILSD
                     HATTGDGTLAALQVLAELVAAGKPASELLHRFDPVPQLLKNVRFAGGKPLEHKDVVAA
                     IAEGEAVLNGRGRLVIRPSGTEPLIRVMAEGDDAGQVEQVVDMICDAVRAAAA"
     gene            2532..3779
                     /locus_tag="VN034_07720"
     CDS             2532..3779
                     /locus_tag="VN034_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWV60524.1"
                     /translation="MKSAWLILSALIAGMLLGIGVELVSPAAGTASLPWIEPIGLLWL
                     NALKMTIVPLIVALLITGITATADAARAGTLAFRAVMTFLGAIFLSGLMSLLMTPLLL
                     RLFPLSPGAAEALRHGLGGTTEAGPSPTFGDFLLSLIPTNPIAAAAETAILPLIVFTT
                     IFAFAITRLEPPQRATLSGLFKALGDAMLIVIGWVLALAPIGVFALGYALAVKAGFAA
                     FGGLIHYVLILSGIGVSCIILGLLLAWFVVGIAPPRFLRAMVPTLAVAISTQSSLASL
                     PAMLKSSEELGVDPKKADVVLPLAVALFRFTSPAMNLAVVVYVAWLFGVELTPWEMAI
                     GLAVAIAAALSSVSLPGSISFVTSIAPIAVSMGVPVAPLGLLVAVETFPDIFRTIGNV
                     IGDVAATKYAADGVENEPIGETP"
     gene            3776..4771
                     /locus_tag="VN034_07725"
     CDS             3776..4771
                     /locus_tag="VN034_07725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWV60525.1"
                     /translation="MKYRKLGHGLEVSAIGIGCMPMIRGGNILYGEAADLDESTRTIH
                     RAIDLGVTFFDTAQIYGPFSNEELLGEAIRGKRDGLVIATKFGFRFEGKQITGVDGSP
                     ANARAACEGSLQRLGIDTIDLFYQHRVDPNVPIEEVVGGMMELVKEGKVRHIALSEAG
                     PETLRRAAKAAPITALQSEYSIWERDIEDEILPVCREHGIGFVPYSPLGRGFLAGAVR
                     SRDELPEGDWRRNDPRYSDENLPANLAIVDAIGAVADKHGVSKAQVALAWLLAQGPDI
                     VPIPGTKRRATMEDSVAAAEVTLTADDLAAIDAAAPKGGTSGPRYGEQGMRMVRL"
BASE COUNT          803 a         1761 c         1572 g          890 t
ORIGIN      
        1 ggttcgagcg gcatcccctg cgccaaaccg cacgcgatcg cgctcgcgag cgtgcagccg
       61 gtaccgtgac tgtgcggcgt gtcgatgcgc ggcgcttccc agcgcgcgac ctcgccctcg
      121 cccgtttcga gcaatcggtc gatcaccgtc gcaccctcgg catggccgcc cttgacgagc
      181 agcgagcagc cgtgcgcgag caccgcctcc tcgccgccca aggcttcgag ttcgggcagg
      241 ttcggcgtcg cgaccatcgc gcgatcgagc aaggccttga acgccgcgat cgtcgcttca
      301 tcggcgagca ccgacccgct cgacgcgatc atcaccggat cgaacaccag cgccgcttgc
      361 gcgaggtcgg gccgcgacag ccgctcggcg accgccgtcg ccgtttcggc gctgccgatc
      421 atgccgatct tgaccgcgtc gacgccgata tcggacgcga cgctgtcgat ctgcgcgagc
      481 accatatcgg tcgggaccgg atgcacgccc tgcacgccca acgtattctg cgcggtgatc
      541 gcggtgatcg cggtcatcgc atggccgccg agcatcgtca ccgccttgat atcggcctgg
      601 atccccgcgc cgccgccgct atccgacccg gcgatgatca ggacgcgtgc ggtcatcgaa
      661 accctccgtc gtcccagcga aagctgggac cgctattggg catagtccgc cgcatgacgc
      721 accagatccc agctttcgct gggatgacgt gagagtaggt ggtcacgcct tatgctttcg
      781 ctccgtcgaa gcggggaatt tcgccagcgc cgcgccctcg cgcagcggcc ggtcgagcag
      841 gtcgccgccg caattggggc agcgctcgtc gagcttgtcg gcgcagttcg cgcagaaagt
      901 gcactcgaac gagcagatga aggcgccgtg aaggtcggcg ggcaaatcgc ggccgcactt
      961 ttcgcaatcg ggtcgcattt caagcatagt aaatctcttt cagttttcaa aaacaaatgg
     1021 tcaggccgcc gccgcgcgca ccgcgtcgca gatcatgtcg acgacctgtt cgacctgccc
     1081 ggcgtcgtcg ccctcggcca tcacgcggat caagggctcg gtgcccgacg gacggatgac
     1141 cagacggccg cgcccgttga gcaccgcctc gccctcggcg atcgccgcga cgacatcctt
     1201 atgctcgagc ggcttgccgc ccgcgaagcg aacattcttc aaaagctgcg gcaccggatc
     1261 gaaccggtgg agcaattcgc tcgcgggctt gccagccgcg acgagctcgg cgagcacctg
     1321 caacgccgcg agcgtgccgt cgccggtcgt cgcatggtcg gacaggatca tatgccccga
     1381 ctgttcgcca ccgacattga agccgccctc cttcatccgt tcgagcacat agcgatcgcc
     1441 gaccttggtg cgttcaagcc gcagcccctc gccctccaga aagcgttcga ggccgaggtt
     1501 cgacatcacc gtcgcgacca cgccgccgcc cttcagccgc ccctgccgcg cccagctcgc
     1561 gccgatcagc cccatgatct ggtcgccgtc gatgatccgt cccttttcgt cgacgacgat
     1621 cagccggtcg gcgtcgccgt cgagcgcgac gccgatgtcg gcgccgctcg cgaccaccgt
     1681 ttcctgcagc agcgcgggcg cggtcgatcc gcatttgtcg ttgatattgg tgccattggg
     1741 ctcgaccccg atcgcgacga cgtcggcgcc cagctcccag aacaccgtcg gcgcgctgtt
     1801 atacgccgcg ccattggcgc aatcgagcac gatgcgcagc ccgtcgaggc ggaccgattc
     1861 gggcaggctc tgtttcaccg catggatata gcggccgcgc gcgtcgtcga tgcgcttggc
     1921 gcgcccgatg tgcgccgcat cggcgagctt cggctcgacg gtcagcagat gctcgatctg
     1981 cgcctcgtcc tcgtcggaca atttatagcc gtcggggccg aacagcttga tcccgttgtc
     2041 gcgaaacggg ttgtggctcg ccgagatcat cacgccgagg tcggcgcgca tcgaacgcgt
     2101 cagcatcgcg atcgccgggg tcggcatcgg gccgacctgc accacgtcca tgccgacgct
     2161 ggtgaaaccc gcgacgagcg cgttttcgag catatagccc gacaagcgcg tgtccttgcc
     2221 gatcacgacg cgatgcttgt gtgtcccgcg caggaaatgc gcgcccgccg ccatgccgac
     2281 gcgcatcgcg acctccgccg tcatcgggac ctggttggtc aggccgcgaa tcccgtcggt
     2341 tccgaaaaac ttgcgcatag tccctctgct acctaaccac ggccggcaat gcctgccccg
     2401 gcttcccacg ggatggcccc cgcggagcat ttcccggccc aaaaaccggt tcccgtttgt
     2461 ttcgggaggt gctctaagtc acccaaattt gcttggcaag ctggccagaa aaatcgccgg
     2521 gagaatccgt cttgaaatcc gcttggctca tcctctcggc gctgatcgcc ggcatgcttc
     2581 tcggcatcgg cgtcgagctg gtctcgcccg ccgccggaac cgcatcgctg ccgtggatcg
     2641 aaccgatcgg cctgctctgg ctcaacgcgc tcaagatgac gatcgtgccg ctgatcgtcg
     2701 cgctgctgat caccggcatc accgccaccg ccgacgccgc gcgcgcgggt acgctcgctt
     2761 ttcgcgcggt gatgaccttc ctcggcgcga tcttcctctc cggcctcatg tcgctcttga
     2821 tgacgccgct gctgctgcga ctctttccgc tttcacccgg cgcagccgag gcgctgcgtc
     2881 acgggcttgg cggcacgacc gaggccgggc cgtcgccgac cttcggcgat ttcctgctgt
     2941 cgctcatccc gaccaacccg atcgccgcgg cggccgaaac cgctatcctg ccgctgatcg
     3001 tcttcaccac catctttgcc ttcgcgatca cccgcctcga accgccgcag cgcgcgacgc
     3061 tctcggggct gttcaaggcg ctcggcgatg cgatgctgat cgtcatcggc tgggtgctcg
     3121 cgctcgcgcc gatcggcgtc ttcgcgctgg gctatgcgct cgcggtcaag gcggggttcg
     3181 ctgcgttcgg cgggctcatc cattatgtgc tgattctctc ggggataggc gtcagctgca
     3241 tcattctcgg gctattgctc gcctggttcg tcgtcggcat cgcgccgccg cgtttcctgc
     3301 gcgcgatggt gccgacgctc gcggtcgcga tcagcacgca aagctcgctc gccagcctgc
     3361 ccgcgatgct gaaatcgtcc gaggagctcg gcgtcgatcc caagaaggcc gacgtcgtgc
     3421 tgcccctcgc ggtagcgctg ttccgcttca ccagcccggc gatgaacctc gcggtcgtcg
     3481 tctatgtcgc ctggctgttc ggggtcgagc tgacgccgtg ggagatggcg atcggcctcg
     3541 cggtcgcgat cgccgccgcg ctctcctcgg tcagcctgcc cggctcgatc agcttcgtga
     3601 cctcgatcgc gccgatcgcg gtgtcgatgg gggttcccgt cgcaccgctg gggctgctcg
     3661 tcgcggtcga gacctttccc gacatcttcc ggacgatcgg caatgttatc ggcgacgtcg
     3721 cggcgaccaa atatgccgcc gacggtgtag agaatgaacc cataggagaa acgccatgaa
     3781 atatcgcaag ctcggccacg gcctcgaagt ctccgcgatc ggcatcggct gcatgccgat
     3841 gatccggggc ggcaacatcc tttatggcga ggctgcggac ctcgacgaat cgacgcgcac
     3901 gatccaccgc gcgatcgacc tcggcgtcac tttcttcgac accgcccaaa tctatggtcc
     3961 gttcagcaac gaggaactgc tcggcgaggc gatccggggc aagcgcgacg ggctcgtcat
     4021 cgcgaccaaa ttcggcttcc ggttcgaggg caagcagatc accggcgtcg acggctcgcc
     4081 cgccaatgcc cgcgcggcgt gcgaaggctc gctccagcgg ctcggtatcg acacgatcga
     4141 ccttttctac cagcaccgcg tcgatcccaa tgtgccgatc gaggaggtcg tcggcgggat
     4201 gatggagttg gtgaaggagg gcaaggttcg ccacatcgcg ctttccgaag ccgggcccga
     4261 aacgctccgc cgcgccgcga aggccgcgcc gatcaccgcg ctgcagagcg aatattcgat
     4321 ctgggaacgc gatatcgagg acgagattct tcctgtctgc cgcgagcatg gcatcggctt
     4381 cgtcccctat tcgccgctcg gccgcggctt cctcgcgggc gcggtgcgca gccgcgacga
     4441 gcttcccgag ggcgactggc gccggaacga tccgcgctat tcggacgaga atctccccgc
     4501 caacctcgcg atcgtcgatg cgatcggcgc ggtcgcggac aagcatggcg tatcaaaggc
     4561 gcaggtcgcg ctcgcgtggc tgctcgcgca agggcctgac atcgtcccca tccccggcac
     4621 caagcgccgc gcaacgatgg aagacagcgt cgcggccgca gaggtgacgc tgaccgccga
     4681 cgatcttgcc gcaatcgacg cagcggcacc gaagggcggc acaagcggcc cgcgctacgg
     4741 cgagcaaggc atgcggatgg tgcgtctgta gggactgatc gccttcgacc gaatgcagcg
     4801 cttttactgt cgtcaccccg gacttgatcc gggatccaga cttccacgct acgatgcccc
     4861 ccgggtcaag tcccgggtga cgatgaagga tcggcgacaa cgacggatcg agaacgttgg
     4921 cgccgttccg ggtcgctgac tcaaaacgga ctagcagaga caaggacgat gacgatggcc
     4981 gaagtgctgg acgaggggcc gttctttcac ggcacggtcg ccgatt
//
LOCUS       DATMSU010000283         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_80616, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000283 DATMSU010000000
VERSION     DATMSU010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80616"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /gene="dapF"
                     /locus_tag="VN034_07730"
     CDS             complement(<1..691)
                     /gene="dapF"
                     /locus_tag="VN034_07730"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWV60526.1"
                     /translation="MADRFTKMHGLGNDFVVIDARDHGVEMTPARAHAIADRRHGIGC
                     DQLILLEPSASADVKMRIFNADGGEVEACGNATRCVATLIGKPAVIETLGGMLRVTPA
                     DGGAEVTLGEPVFDWEHIPLAMPMDTRDMPVAWDELEHGAAVNVGNPHIVFFVPEADA
                     VALDQLGPRIETDPLFPERVNVNVASLDGENQLQLRVWERGVGLTQACGTGACATAVA
                     AIRAGKVQSPVT"
     gene            complement(724..1134)
                     /locus_tag="VN034_07735"
     CDS             complement(724..1134)
                     /locus_tag="VN034_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HWV60527.1"
                     /translation="MRRVPSEMTERARQLRRDATPAERKLWRILSRYRPAFTRQLVVG
                     SYIVDLACREAKLAIELDGSQHLDQQEYDGKRTAYLESMSWRVIRLWNSEVMTNPDGA
                     AEYLLAQAAECLGGTHPQPLPSREGRIRKPRTPR"
     gene            complement(1207..>2374)
                     /locus_tag="VN034_07740"
     CDS             complement(1207..>2374)
                     /locus_tag="VN034_07740"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWV60528.1"
                     /translation="VAAERISAVLPPTATKARLGGDEFVAMLAFEPSARADIDALAAE
                     LVAALDNMITHDAQQIRIGASLGIALADDASMTMETMVRQADIAMYHCKDEGRNRFCW
                     FEVGMEMAVQVRNQIETGIREGMPRGEFVPHFEPQVDIASGRLLGFEMLMRWESPEYG
                     MIPPERFIPVAEESGLIGELSLQVIREAMEIAKRWDPSIMLAVNISPQQLKDPWFSQK
                     LTKLLVETGFPAAQLEVEITESSLFENLPLVRSIVTSLKNQGVSLSLDDFGTGYSSLS
                     HLRALPFDRIKIDRSFIAAMRGSPDAQAIVVAIVRLGESLAMPITAEGVEDEATAIEL
                     TRLGCSKGQGWHFGRAASAADTDRLLAERGLLRAPMVPEVGSDTSEGTPLRKSA"
BASE COUNT          380 a          789 c          781 g          424 t
ORIGIN      
        1 ccgtcaccgg cgactgcacc ttgcccgcgc ggatcgcggc aactgcggtc gcgcacgcgc
       61 cggtgccgca cgcctgtgtc agcccgacgc cgcgctccca gacgcgtagt tgcaactggt
      121 tctcgccgtc gaggctcgcg acattgacgt tgacgcgttc gggaaacagc gggtcggtct
      181 cgatccgcgg gccgagctgg tcgagcgcga cggcgtcggc ttcgggcacg aaaaagacga
      241 tgtgcgggtt gccgacattg actgcggcgc catgctccag ctcgtcccac gcgacgggca
      301 tgtcgcgcgt gtccatcggc atcgcgagcg ggatatgctc ccagtcgaag acgggctcgc
      361 ccaacgtgac ttcggcgccc ccgtcggccg gcgtgacgcg cagcatcccg ccgagcgtct
      421 cgatcaccgc gggcttgccg atcagcgtcg cgacgcagcg cgtcgcgtta ccgcatgcct
      481 cgacctcgcc gccgtcggcg ttgaagatgc gcatcttcac gtccgcactc gccgacggtt
      541 cgagcaggat caactggtcg cacccgatgc catgccgccg gtcggcgatc gcatgcgcgc
      601 gcgcgggcgt catctcgacg ccatggtcgc gcgcgtcgat cacgacgaag tcgttgccga
      661 ggccgtgcat cttggtgaag cggtctgcca tagcgcgcat ttaggggggc gagggcgcga
      721 agtctagcga ggcgtccgcg gtttcctgat cctcccctcc ctggaaggga ggggttgggg
      781 gtgggtcccg ccgaggcact cggcggcctg cgcgagcaga tattcggctg ctccatctgg
      841 attggtcatt acctcggaat tccatagccg gatgacccgc cagctcatgc tttccagata
      901 ggcagttcgc ttgccgtcat attcctgctg gtcgagatgc tggctaccat caagttcgat
      961 agcgagcttc gcctcacggc acgccagatc gacgatatag gacccaacga caagttgtct
     1021 ggtgaaggct ggccggtaac gggagaggat gcgccacaat tttcgttctg ccggcgttgc
     1081 gtctcgccgc agctgtcgag cgcgttcagt catttccgat ggcacgcggc gcatgacgga
     1141 atgatagaac ggcgagctag gctcgccaac ccacccccag cccctccctt gcagggaggg
     1201 gaggacttat gcgctctttc gcagcggcgt tccctcgctg gtgtccgatc cgacctccgg
     1261 caccatcggc gcgcggagca ggccgcgctc ggcgagcagg cggtcggtgt cggccgccga
     1321 cgccgcgcgc ccaaagtgcc agccctgacc cttcgaacag ccgagccgcg tcagttcgat
     1381 cgcggtcgcc tcgtcctcga ccccctcggc ggtgatcggc atcgccaggc tttcgcccag
     1441 ccgcacgatc gcgacgacga tcgcctgcgc atcggggctg ccgcgcatcg cggcgatgaa
     1501 gctgcggtcg atcttgatgc ggtcgaacgg cagcgcgcgc agatgcgaca gcgagctgta
     1561 gccggtgccg aaatcgtcga ggctcaagga cacgccctga tttttgaggc tggtgacgat
     1621 cgagcgcacg agcggcagat tttcgaacag cgagctttcg gtgatctcga cctcgagctg
     1681 cgcggcggga aaacccgttt cgacgagcag cttggtgagc ttctggctga accacgggtc
     1741 cttcagctgc tgcggcgaga tattgaccgc gagcatgatc gacgggtccc accgcttggc
     1801 gatctccatc gcctcgcgga tgacctgcaa cgacaattcg ccgatcagcc cgctttcctc
     1861 ggcgaccggg atgaagcgtt cgggcgggat catgccatat tcgggcgatt cccagcgcat
     1921 cagcatctcg aagccgagca ggcgtccgct cgcgatgtcg acctgcggct cgaaatgcgg
     1981 aacgaactcg ccgcgcggca tgccttcgcg gatgccggtt tcgatctggt tgcggacctg
     2041 cactgccatc tccattccga cctcgaacca gcagaagcgg ttccggcctt cgtccttgca
     2101 atgatacatg gcgatgtcgg cctggcggac catcgtttcc atcgtcatgc tcgcatcatc
     2161 ggcaagcgcg atgccgagcg acgcgccgat ccggatctgc tgcgcgtcgt gcgtgatcat
     2221 attgtcgagc gccgccacca gctcggcggc gagcgcgtcg atgtcggcgc gggcagaggg
     2281 ctcgaaagcc agcatcgcga cgaattcgtc gccgccgagc cgcgccttgg tggcggtggg
     2341 cggcagaacg gccgagatgc gctcggcggc gacc
//
LOCUS       DATMSU010000284         6762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_52312, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000284 DATMSU010000000
VERSION     DATMSU010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52312"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VN034_07745"
     CDS             <1..211
                     /locus_tag="VN034_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV60529.1"
                     /translation="PWALHPLVRGAAESLFPRLADGAMILLDDAARPGERLVARRWQK
                     RWPQIDFAFDRSGTKGTLIGRVPSG"
     gene            267..857
                     /locus_tag="VN034_07750"
     CDS             267..857
                     /locus_tag="VN034_07750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HWV60530.1"
                     /translation="MPRHILVFYGSYRRDRQGIKLARWLVDAIGARGDSAELIDAKAV
                     GLPMLDRMYKEYPKGEAPAAMEELAGKIRTADGFLFVTGEYNWGPQPGLKNLTDHYLE
                     EWFWRPAAIACYSAGPWSGVRAMMGWRDTLGEMGMAVTSSILPVGRIGGAFDAEGNPA
                     GDDGARLRKSFPRFADDLNWWIDAAKAQRAKVDPPY"
     gene            complement(865..2178)
                     /locus_tag="VN034_07755"
     CDS             complement(865..2178)
                     /locus_tag="VN034_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60531.1"
                     /translation="MSDKAAPAPGIASPSATGVSAQTRTMLWILLIVYIFNFLDRQIV
                     NILAEPIKADLGLSDTELGLLAGPAFAVFYALLGIPIARYADKEGTNRVRLIALALAV
                     WSAMTAVCGLAQNFVQLLLARIGVGVGEAGCTPAAHSLISDSVAPEKRSSAIAFYGLG
                     VPIGSLLGLILGGIVNDLYGWRVALMLVGAPGLLLALVVLFVMREPRHHRPVEATTTA
                     ALPQLPARDAMREIFSSRAFLYILIAASVTAFLGYGKALWTISFFIRSHGLSTTEAGL
                     SMAVVLGLAGVFGTWLGGKCADVFGKRDKRHILTLPAYGMAVGAPILFLGYQMDDWRV
                     AVALLIVPTILNSAYYGPAYACVQGLVRPQARAVAASIMLFGQNLIGLGFGPFLFGVL
                     SDALAPAYGEESVRYVLYGAAWLGLIPAFFFWRASLRLGRELKSG"
     gene            complement(2311..2793)
                     /locus_tag="VN034_07760"
     CDS             complement(2311..2793)
                     /locus_tag="VN034_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HWV60532.1"
                     /translation="MDLDAGAIAQTIQLSVTPVFLLVATGSLLNVIAGRLARVVDRSR
                     NLMERWADTEGVEHERIVAELRIADRRMGIINNSILSAVACGIVVCLLVALLFTQAFT
                     GINLGVAASWAFAAAMLLLLVSLILFLVEVRLAVRAIHVPMELLELEGRDRKRRRERP
                     "
     gene            complement(2793..4007)
                     /locus_tag="VN034_07765"
     CDS             complement(2793..4007)
                     /locus_tag="VN034_07765"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HWV60533.1"
                     /translation="MSEDEFYRIKRLPPYVIAEVNAMRAAARAAGEDIIDLGMGNPDL
                     PPPAHVIEKLCEVAMKPDAHGYSQSKGIPGLRRAQANYYGRRFNVDLDPETEVVVTMG
                     SKEGLASLATAITGPGDVVLAPNPSYPIHTFGFIIAGATIRSVPTTPDENYWRALDRA
                     MAFTVPRPSILVVNYPSNPTAEAVDLAFYERLVAWAKENKVWVLSDLAYSELYYDGNP
                     TPSILQVPGAKDVAIEFTSMSKTYSMAGWRMGFAVGNKRLIAAMTRVKSYLDYGAFTP
                     IQAAACAALNGPQDIVAKNRELYQKRRDVMVESFARAGWDIPSPPASMFAWAPLPPAL
                     KEMGSLEFSKQLLTHAKVAVAPGVGYGEDGEGYVRIAMVENEQRIRQAARNIRKFLAM
                     HGVNTPSVAAGG"
     gene            4124..5899
                     /gene="phaC"
                     /locus_tag="VN034_07770"
     CDS             4124..5899
                     /gene="phaC"
                     /locus_tag="VN034_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HWV60534.1"
                     /translation="MNDTGKDDTTEAPNPFMPSPDDLQHWASVMGRAQQMMLEYALGQ
                     ANQGNSAAAGMFDPASWLQNPTTQLWAEQSSKLWEQGVTFWTSLATIQPSFTPETDAR
                     RPDKRFADPDWTANPVFALIRQTYGLLAEQLLTTTRTMQGLDEHARAKMEFAAKNMTD
                     ALSPSNLAITNPEVIRRAVETRGESLLKGLKNMLADLSRGQLSHVDPDAFEVGVNIAT
                     TPGKVIHETDLYQLIQYSPTTKEVFTVPLVIFPPWINRFYILDLNPAKSFVAWAVEQG
                     LSVFMVSWKSADASMGEIVWDDYAAAQVDAVDTVRDLLGVPHVHTIGYCVAGTTLAAT
                     LAMLAARGEADKVKSVTFFTAQVDFELAGDLKMFVDESYLTLLQQLSAGGFLDGRYMA
                     ATFNSLRGRDLIWNYVVSNYLLGNDYPPFDLLYWNGDVTNLPAKWHRQYLVDLYRDNR
                     MVIPNSLTVMATPIDLKKIETPAYIQAGREDHIAPLASVWRLMEHLSGPKTFVLAGSG
                     HIAGVVNPPAAGKYQYWTGDNDAASLDDFVASASETKGSWWPHWIGWIAQQDGAKIAA
                     KGARIPGKGKKKPIEDAPGRYVKQR"
     gene            6023..>6762
                     /locus_tag="VN034_07775"
     CDS             6023..>6762
                     /locus_tag="VN034_07775"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWV60535.1"
                     /translation="MATKMDSAEKAFEAATLETAAKPVAAPAVPAQAAPAAKAAAPVK
                     TKTVAAKAKPVQKKAAKPAAKKVAPKKAAAKTLASKPAKAAPKPAALATKGLKTMNDT
                     VKKFADEAKTRTEALTADFNEKAKDAFAKSSKLAEEAVEFNKANVEAFVEAGKIAAKG
                     IETLGQEGVTFARKSFEDTTAALKGYTAVKTPADFFKLYAENSKKAFDAAVAQTSKTS
                     ELVVKLTNDSFAPISNRVSVITSKMKAA"
BASE COUNT         1354 a         2186 c         2151 g         1071 t
ORIGIN      
        1 gccctgggcc ctgcatccgc tggtgcgcgg ggcggccgaa tcgctgttcc cgcgccttgc
       61 cgacggggcg atgatcctgc tcgacgacgc ggcgcggccg ggcgaacgtc tcgtcgcgcg
      121 gcgctggcag aaacgatggc cgcagatcga tttcgcgttc gaccggtcag gcacgaaagg
      181 cacgctcatc ggccgcgtgc caagcggctg acatcgcgcg gccgagcgtc taggacatga
      241 tgcccgatcc attttgaggt ggtctcatgc cgcgccatat cctcgtcttc tacggcagct
      301 atcgccgcga ccggcagggc atcaagctcg cgcgctggct cgtcgatgcg atcggcgcgc
      361 gcggcgacag cgccgagctg atcgacgcga aggcggtcgg cctgccgatg ctcgaccgca
      421 tgtacaagga atatccgaag ggcgaggcgc cggcggcgat ggaggaactt gcgggcaaga
      481 ttcgcaccgc cgacggcttt ctgttcgtca ccggcgaata taattggggg ccgcagccgg
      541 ggctcaagaa cctcaccgac cattatctcg aagaatggtt ctggcgcccc gcggcgatcg
      601 cctgttattc ggcggggccc tggtcggggg tgcgcgcgat gatggggtgg cgcgacacgc
      661 tcggcgagat gggaatggcg gtcacctcgt cgatcctgcc ggtcgggcgg atcggcggcg
      721 cgttcgacgc cgagggcaat ccggcgggcg acgacggcgc gcggcttcgg aaaagctttc
      781 cccgcttcgc cgacgatctc aactggtgga tcgacgccgc caaggcgcag cgcgccaagg
      841 tcgatccgcc ctactaagtc cgccctagcc cgacttgagt tcgcggccga gccgcaggct
      901 ggcgcgccag aagaagaagg cggggatgag gccgagccac gccgcgccgt agaggacgta
      961 gcgcacgctt tcctcgccat aagcgggcgc gagcgcgtcc gacagcacgc cgaagaggaa
     1021 cggaccgaag ccgagcccga tcagattctg tccgaacagc atgatcgacg ccgcgaccgc
     1081 gcgcgcctgc ggccgcacca gcccctgaac acaggcatag gcgggaccat aataggccga
     1141 gttgaggatg gtcggaacga tgagcagcgc caccgcgacg cgccagtcgt ccatctggta
     1201 gccgagaaag aggatcgggg cgccaacggc catgccatag gcgggcaagg tcaaaatatg
     1261 tcgcttgtcg cgcttgccga aaacgtcggc gcatttgccg ccgagccagg tgccgaatac
     1321 ccctgccagt cccagcacga ccgccatcga cagtcccgct tcggtcgtcg acaggccgtg
     1381 gctgcggatg aagaagctga tcgtccacag cgccttgcca tagccgagga aggcggtgac
     1441 ggacgccgcg atcaatatgt agaggaacgc gcgcgacgaa aagatttcgc gcatcgcatc
     1501 gcgcgcggga agctgcggca gcgcagcggt agtggtcgcc tccacgggcc ggtgatgccg
     1561 cggttcgcgc atcacgaaca gcaccaccag cgcgagcaac agccccggcg ccccgacgag
     1621 catcagcgcg acgcgccagc cgtaaagatc gttgacgata ccgccgagaa tcagcccgag
     1681 cagcgagccg atcggcacgc cgaggccgta aaatgcgatc gcagacgagc gcttttcggg
     1741 cgccacactg tccgaaatca gcgaatgcgc ggcgggcgtg caccccgcct cgcccacccc
     1801 gacaccgatc cgcgccaaaa gcagttgcac gaaattctgc gccagcccgc acaccgcggt
     1861 catcgccgac cagaccgcga gcgcgagcgc gatcagccgc acccggttcg ttccctcctt
     1921 gtcggcatag cgcgcgatcg ggatgcccag cagcgcatag aatacggcga atgcggggcc
     1981 ggcgagcagg ccgagttcgg tgtccgaaag cccgaggtcg gccttgatcg gttcggcgag
     2041 gatattgacg atctgccggt cgaggaaatt gaagatatag acgatcagca ggatccacag
     2101 catcgtccgc gtctgtgcgg aaacgccggt ggctgacggc gaagcgatac ccggggccgg
     2161 tgcggctttg tcagacattc atctctcccg ccgccatcgg agcagaccgg agggaccggt
     2221 gtcaacgtcc ttgcggcgtg ccggaaatgc ccgttcgcaa ttgccgttac ggcgaccgcc
     2281 gtgcagcaca tggaaaaatt tttcggccgg tcagggccgt tcgcgccgcc gcttccgatc
     2341 cctgccctcc agctcgagca attccatcgg gacgtggatc gcgcgcaccg cgagacggac
     2401 ctcgacgagg aaaaggatca gcgagacaag cagcaacagc attgccgccg caaaggccca
     2461 ggacgccgcg acgcccaggt tgatgccggt aaaggcctgg gtgaacagca atgcgacgag
     2521 caggcagacg acgatgccgc acgccacggc cgagaggatc gaattgttga tgatccccat
     2581 ccgccggtcg gcgatgcgca gttcggcgac gatgcgttca tgctcgacgc cttcggtgtc
     2641 ggcccagcgt tccatcaggt tgcgcgagcg atcgacgacg cgcgccagcc gccccgcgat
     2701 gacgttgagc aggctgccgg tcgcgaccag cagaaacacc ggcgtgaccg acagctgaat
     2761 cgtctgcgca atcgcgccgg cgtcgagatc catcatcccc cggctgcgac cgacggcgtg
     2821 ttgaccccgt gcattgccag gaatttgcgg atgttgcgcg ccgcctgccg gatgcgctgc
     2881 tcattctcga ccatcgcgat gcggacatag ccctcgccgt cctcgccata gccgacgccc
     2941 ggcgcgaccg cgaccttggc gtgggtgagc aactgcttcg aaaattcgag gctgcccatt
     3001 tccttgagcg cgggtggcag cggcgcccag gcgaacatcg acgcgggcgg gctcggaatg
     3061 tcccagccgg cgcgcgcaaa actttcgacc ataacgtcgc ggcgcttctg gtaaagctcg
     3121 cggttcttgg cgacgatgtc ctgcggcccg ttgagcgcgg cgcacgccgc ggcctggatc
     3181 ggcgtgaagg cgccataatc gagataggac ttcacgcgcg tcatcgccgc gatcagccgc
     3241 ttgttgccga ccgcaaagcc catgcgccag cccgccatcg aataggtttt cgacatcgag
     3301 gtgaactcga tcgcgacatc cttcgcgccc ggcacctgca ggatcgaggg cgtcggattg
     3361 ccgtcgtaat aaagctccga ataggcgagg tccgacagca cccagacctt attctccttc
     3421 gcccaggcga cgagccgctc atagaaagcg agatcgacag cctcggcggt cgggttcgac
     3481 ggatagttga ccacgaggat cgacgggcgc gggacggtga aggccatcgc gcggtcgagc
     3541 gctcgccagt aattctcgtc gggggtcgtc ggcacgctgc ggatcgtcgc gccggcgatg
     3601 atgaagccga aggtgtggat cggatagctc gggttcggcg cgagcacgac gtcgccgggg
     3661 ccggtaatcg cggtcgcgag gctcgcgagc ccttccttcg agcccatcgt cacgacgacc
     3721 tcggtttcgg gatcgaggtc gacgttgaag cggcggccgt aataattggc ctgcgcgcgg
     3781 cgaaggcccg gaatgccctt cgactgcgaa tagccgtgcg cgtcgggctt catcgcgact
     3841 tcgcacagct tttcgatcac atgcgcgggc ggcggcaggt cggggttgcc catccccagg
     3901 tcgatgatgt cctctcccgc ggcgcgggcg gccgcacgca tcgcattcac ttcagcgatg
     3961 acgtagggcg gcaggcgctt gatgcgatag aattcatctt cggacatggg aaactagaac
     4021 aactcctttg cgttaactgt tgaattgacg cgcgcggcaa tctcgccagc cgcatcgcgg
     4081 cttgttatag gcattatatc cgcactgacc agcaggaacg agcatgaacg atacgggtaa
     4141 ggacgacacg accgaagcac cgaatccctt catgccgtcg cccgatgacc tgcagcattg
     4201 ggccagcgtc atgggccgcg cgcagcagat gatgctcgaa tatgcgctgg gtcaggcgaa
     4261 ccagggcaac agcgccgctg cgggtatgtt cgaccccgca agctggctgc aaaatccgac
     4321 gacccagcta tgggccgaac aatcgtcgaa gctgtgggaa cagggcgtca ccttctggac
     4381 ctcgctcgcg acgatccagc cctccttcac gcccgaaacc gatgcgcggc gaccagacaa
     4441 gcggttcgcc gatcccgact ggaccgccaa cccggtcttc gcgctgatcc gtcagaccta
     4501 tggcctgctc gccgagcagt tgctcacgac cacccgcacg atgcagggac tcgacgagca
     4561 tgcgcgcgcg aagatggaat tcgccgcgaa gaatatgacc gacgcgctgt cgccctcgaa
     4621 ccttgcgatc accaatcccg aagtgatcag gcgcgcggtc gagacgcgcg gcgaaagcct
     4681 gctcaagggt ttgaagaata tgctcgccga cctgtcgcgc gggcagctct cccacgtcga
     4741 ccccgacgcg ttcgaagtcg gcgtgaatat cgcgacgacg ccgggcaagg tgatccacga
     4801 aaccgatctc tatcagctga tccagtacag cccgacgacg aaggaagttt tcaccgtccc
     4861 gctcgtcatc ttcccgccgt ggatcaaccg cttctacatt ctcgacctca accccgcgaa
     4921 aagcttcgtc gcctgggcgg tcgaacaggg actcagcgtt ttcatggtgt cgtggaagtc
     4981 ggccgacgcg tcgatgggcg agatcgtgtg ggacgactat gccgcagcgc aggtcgacgc
     5041 ggtcgacacg gtgcgcgacc tgctgggcgt gccgcacgtc cacaccatcg gctattgcgt
     5101 cgcgggcacg acgcttgccg cgacgctcgc gatgctcgcg gcgcgcggcg aggcggacaa
     5161 ggtcaagtcg gtgactttct tcacggcgca ggtcgacttc gagcttgcag gcgacctcaa
     5221 gatgttcgtc gatgaaagct atctgacgct gctgcagcaa ttgtcggcgg gcggttttct
     5281 cgacgggcgc tatatggccg cgaccttcaa cagcctgcgc gggcgcgatc tcatctggaa
     5341 ctatgtcgtg agcaattacc tgctcggcaa cgactatccg ccattcgacc ttctctactg
     5401 gaacggcgac gtcaccaacc tgcccgcgaa gtggcaccgg caatatctgg tcgaccttta
     5461 tcgcgacaat cgcatggtga tcccgaacag cctgacggtg atggcaacgc cgatcgacct
     5521 caagaagatc gagacccccg cctatatcca ggcgggccgc gaggatcata tcgcgccgct
     5581 cgcgagcgtg tggcggctga tggagcatct gtcggggccc aagaccttcg tcctcgcggg
     5641 ctctgggcat attgccggcg tggtcaatcc gcccgcggcg ggcaaatatc aatattggac
     5701 cggcgacaat gacgccgcgt cgctcgacga ttttgttgca agcgcgtccg aaaccaaggg
     5761 cagctggtgg ccgcactgga tcggctggat cgcgcagcag gatggggcga aaatcgccgc
     5821 caaaggcgcg cgcatcccag gaaaaggcaa gaaaaagccg atcgaggacg cacccggccg
     5881 ctacgtcaaa cagcggtaat atctggcgaa tacatcggca ctcgggccgg cggtgcagca
     5941 tttttgtgcg ctgcacaaat ttttgcttga aatcgccgat ccactcctat attgtgcagt
     6001 gcaacataaa ggagttgtcc cgatggctac caagatggat agtgccgaaa aggctttcga
     6061 agccgcgacg ctggaaaccg ccgccaagcc ggtggccgct cctgccgttc ccgcccaagc
     6121 tgcacccgca gcgaaggccg cggcacccgt gaaaaccaag actgtcgcgg cgaaggccaa
     6181 gccggtccaa aagaaagccg caaagccggc agccaagaag gtcgccccca agaaggcggc
     6241 tgcgaagacc cttgcctcca agcccgcgaa agctgcgccg aaaccggccg ctctcgccac
     6301 caaaggattg aagaccatga acgacaccgt caagaagttc gccgacgaag ccaagacccg
     6361 caccgaagcc ctgaccgccg acttcaacga gaaggccaag gacgcgttcg cgaagtcgag
     6421 caagctcgcc gaggaagccg tcgaattcaa caaggcgaac gtcgaagcct tcgtagaagc
     6481 cggcaagatc gctgccaagg gcatcgaaac gctcggccag gaaggcgtca ccttcgctcg
     6541 caagagcttt gaagacacga ccgccgcgct gaagggttac accgccgtca agaccccggc
     6601 cgacttcttc aagctctatg ccgaaaacag caagaaggcg ttcgacgccg ctgtcgcgca
     6661 gacctcgaag accagcgaac tcgtcgtcaa gctgacgaac gacagcttcg cgccgatctc
     6721 gaaccgcgtt tcggtcatca cttcgaagat gaaggccgcc ta
//
LOCUS       DATMSU010000285         4944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_137602,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000285 DATMSU010000000
VERSION     DATMSU010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137602"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1084)
                     /locus_tag="VN034_07780"
     CDS             complement(188..1084)
                     /locus_tag="VN034_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV60536.1"
                     /translation="MNIEDLRTFVEVADAGGVTPAARRLGISKSMVSRRLVRLEAELS
                     VQLLARTTRGAALTEAGATFREHAARVVAEVETAKEVILPAGELRGRLRVAAPLSFGP
                     THFAPVLAEMALRHPELHIHTSYTDRFVDLIAEGFDCAIRVGKLPDSNLIARKIGPLN
                     AKYVASPSYIERHGSPETPEELMTHEALMQGTETWPALDGDKVIGIRPHGRFKADNGA
                     ALVAAALAGVGVAVLPLDLIKEHLDSGALVPVMTRFPVPEHGIYIVRPSGLHPARKIR
                     VLTEMLIECFEGPPHSVGRTSR"
     gene            1230..1880
                     /locus_tag="VN034_07785"
     CDS             1230..1880
                     /locus_tag="VN034_07785"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWV60537.1"
                     /translation="MTFRNGLASLLRPEDSVLVLIDHQPYQLANLNSHDPQAVVNLTT
                     ALAKLARAFNVPTILTSVIAGRGGLLFKQITDVFPGQEVIDRTWVNTWQDEKVVDAVK
                     ATGRKQLIIAGLWTEVCVAMPVIQAAGEGWDVTVITDASGGISKESHEVAIQRMIAAG
                     ANVMTVMALAGEWQRDWARTEHVEELTEILIQHFGGSGIAYLWEQQLLNTPVESAD"
     gene            2089..2544
                     /locus_tag="VN034_07790"
     CDS             2089..2544
                     /locus_tag="VN034_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60538.1"
                     /translation="MRLQKKLTPLWFFGSASDTGKLQTNKRRTARTTAAVQAERPPLI
                     ERFSDSGALHSGETPFSLGTNGKSADSKSGCPLLDIGSQRTLGRRDMQSRLFIALPVF
                     LALSGCSEEIQAQDQPPTLPGETRPSEANEVQGTESEADIELRNPVGLD"
     gene            2772..3410
                     /locus_tag="VN034_07795"
     CDS             2772..3410
                     /locus_tag="VN034_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV60539.1"
                     /translation="MESSSHSRTAGTRRIDNIEGGAKSDRRGAERYRTVYRIARVQRA
                     DDVGLWRVRNISNEGLMLAADIPATVGETLGISLSETSTLRGKIIWAERGHCGVAFDG
                     PIDAAAVLRGLADEQRAEGYRALRLPVEVEAIIALSDDARPIDLVDISQHGAGFRYDR
                     VLDPGTEVDLILPGGDLRRRALVRWSRGQRGGLWFTQQLDRAMLESIALLQR"
     gene            3565..3726
                     /locus_tag="VN034_07800"
     CDS             3565..3726
                     /locus_tag="VN034_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60540.1"
                     /translation="MILRNPDGNNDVEWRRCVIIFPLIKVPDLTQDDLSQSGLLAPPH
                     QRYGYPFPS"
     gene            4053..4634
                     /locus_tag="VN034_07805"
     CDS             4053..4634
                     /locus_tag="VN034_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HWV60541.1"
                     /translation="MRIAIIDTSAGRAAVISDGLREAGLEDLVLVDTARPLVAQIEAA
                     TPDVVLVNLENPSRDLLEDFFAMSRALARPIAMFVDQSDAEATGAAIDAGVSAYVVDG
                     LSKQRIKPVIDLAIRRFQAFSTLQRELDEAKNALAERATIDRAKAILMKRRQIDEPAA
                     YALLRGQAMRTNRRIGEIAEAIVTSEALLGDME"
     gene            4631..>4944
                     /locus_tag="VN034_07810"
     CDS             4631..>4944
                     /locus_tag="VN034_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWV60542.1"
                     /translation="MTESFRIGFLPLVDAALPILAHELGFAAREGIAIELVRDMTWAT
                     VRDRLLYGHTDAAHMIAPLAIATPLGRDRPPVALAVPFVLGLNGNAVTFSTRLAREVG
                     LG"
BASE COUNT          984 a         1552 c         1498 g          910 t
ORIGIN      
        1 gagagctctt cctttcagga aaacagagat cttcatctgc gcccgcccgg gttcgttcgg
       61 gcgggcgtcc ttttttggct cgccgttcga taactgctcg aactgtcgag acaggactga
      121 tcaccgccga ccttttttct aatgggacgc aacaggctcc ggcgtcagat tgacagctat
      181 tgccacttca ccgggatgtc ctgccaacac tgtgcggtgg cccctcgaag cactcgatca
      241 gcatctcggt aaggacccgt atcttgcgcg ccggatgcag cccagaggga cggacgatat
      301 agatgccgtg ctcaggaaca ggaaagcgcg tcatcactgg taccagtgcg cctgagtcaa
      361 gatgctcttt gattaggtcc aggggcaaaa cggcaacacc cacgcccgcc agcgcggcgg
      421 caacgagcgc ggcaccgttg tcggccttga accgcccatg gggccgtata ccgatgacct
      481 tgtcgccatc cagcgcgggc catgtctccg tcccctgcat cagcgcctca tgggtcatga
      541 gttcctcagg cgtttccggc gatccgtgcc tttcgatata agatggcgaa gcgacatact
      601 tcgcgttgag cgggccaatc ttccttgcga tcaggttgga atcgggcagc ttaccgacgc
      661 ggatcgcgca atcaaatccc tccgcgatca ggtcgacaaa ccgatccgta tagctcgtat
      721 ggatatgcag ttccggatgg cgcagcgcca tctcagcgag aacgggcgca aagtgcgtgg
      781 gtccaaacga tagcggtgcc gccacgcgga ggcgtccgcg cagttcgccc gccgggagaa
      841 taacctcctt ggcagtctcg acctcggcaa caacacgggc tgcgtgctcc ctgaacgttg
      901 ctccggcttc ggtgagcgca gccccgcgtg ttgtgcgggc gagaagttgg acactcagtt
      961 ccgcttcgag ccgcacgagc cgccggctga ccatcgattt ggatatgccg agccggcgcg
     1021 cggcaggcgt gacacctccc gcatcggcga cttctacgaa ggtccgcaaa tcctcgatat
     1081 tcatccggcg ttccccattc cgcgacacag cacatcagca agcgatgcta ccgcgacgga
     1141 aagaggaatg gcaacacaca cgcaggtgat gtcgcctcgg tcgatgtccc tggcgatcca
     1201 cgcaaccaac tgcagcagag agtaacgaca tgacattccg caacggcctc gcttcgcttc
     1261 ttcgcccgga agactcggtc ctcgttctga tcgaccacca gccctatcag ctcgcaaacc
     1321 tgaacagcca cgatccgcaa gccgtggtca acctcacgac cgcgctggcg aagctagcga
     1381 gggccttcaa tgttccgacc attctcacca gcgtgatcgc gggtcgcggt ggcctcctct
     1441 tcaagcagat caccgacgta tttccgggcc aggaggtgat cgaccggact tgggtgaaca
     1501 cctggcagga cgaaaaggtt gtggatgcgg tcaaggcgac cggccgcaag cagctgatca
     1561 tcgcgggcct ttggaccgaa gtctgcgtcg cgatgcctgt gatccaggcc gccggcgaag
     1621 gctgggacgt gacggtaatc accgacgcgt cgggcggcat ttcgaaagag tcacacgagg
     1681 tcgccatcca gcgaatgatc gcggcgggcg cgaacgtgat gaccgtgatg gcgctcgctg
     1741 gcgaatggca gcgcgactgg gcgcgcaccg agcatgtcga agaactgacc gagatcctca
     1801 tccagcactt cggcggcagc ggcatcgcgt atctgtggga gcagcaactt ctgaacactc
     1861 ccgtcgaaag cgcggactga acggtaaggt ggcgaacaac agccgagcgc gcttccgcaa
     1921 acgatcgaca tatggatgtt cgccaccaac cctctccgac cctgggcttc aaccaggcgt
     1981 ctgcgctcct gaacatacag ctgactggac tgatactgga agcttgggga tggtccgcgt
     2041 ctcgactaaa tttgagacca gtggacctcg gcgggatggg ggcagagcgt gaggctgcag
     2101 aagaaattga cgccgctttg gttttttgga agcgcaagcg acaccggcaa gctccagaca
     2161 aacaagcgcc gaaccgctcg cactaccgcc gctgtacaag ccgagcgccc gcctcttata
     2221 gagcggtttt cagattccgg cgcactccat tcaggggaaa cgcctttctc tcttggaacc
     2281 aacggcaagt ctgcggattc caagagcggt tgccccctgc tcgatatcgg cagccaacgg
     2341 actttgggga ggcgggacat gcaatcgagg cttttcatcg cgctaccagt ttttctggct
     2401 ctttcgggct gcagtgagga aatccaggct caggatcagc caccgacgct gcccggcgag
     2461 acgaggccga gcgaagcgaa cgaagtgcag ggcaccgaaa gcgaagctga catcgaactt
     2521 cggaatccgg tcggacttga ctgacgaata ccgttcgatc agcgcgcgcc atgctcgagc
     2581 tgcgccgggc gtggatctat agaaaattaa gcttgccgta acggaaactt gctttagtca
     2641 aggctatcag gaccgcaatt agctttcgtg gcgcgtggga gcgcgacgag atgtcaacgt
     2701 cgagcgcgaa gcccggcggt cgtgcgcggt tctgctttcg atggaacccg cacccatgaa
     2761 gggagggcca gatggagagc agcagccata gccgtacagc gggtacacgg cgcatcgata
     2821 atatcgaagg tggcgccaag agcgatcggc gcggagcgga acgctatcgg acagtgtacc
     2881 ggattgcgcg tgtgcagcgt gcggacgatg tcggcttgtg gcgcgtgcgc aatatatcga
     2941 acgaggggct gatgctcgcc gctgatattc cggccactgt cggcgaaacg ctcggcattt
     3001 cgctctccga gacgtcgacc ctccggggca agatcatctg ggcggagcgc ggtcattgcg
     3061 gagtcgcgtt cgacgggccg atcgacgccg ccgcagtgct gcgggggctc gccgatgaac
     3121 agcgcgcgga gggctatcgg gcactgcgac tgccggtcga ggtcgaggca atcattgcac
     3181 taagcgacga tgcccgcccg atcgacctcg tcgatatctc tcagcatggt gccggctttc
     3241 gctatgaccg cgtgctcgat cccggcaccg aggtcgattt gatcctccct ggtggcgatc
     3301 tgcgacgccg ggcactggtg cgctggtctc gtggccagcg cggcggtctt tggttcaccc
     3361 agcaactcga tcgcgctatg ttggagagca tcgcgctcct gcagcgatag cgcgcgcgcc
     3421 tctgcgagac gtcgaaatgc aatccgcccg cggaagttgc aaccgccgcc ccggcacggg
     3481 tgatgatctg gcgatttcga aatatatccg tcccgatgac ctctgcatcc cagctgttgg
     3541 aaatcctatc gaaagcgata acccatgatc ctgcgcaatc ccgacggcaa caatgatgtc
     3601 gagtggcgcc gctgcgtcat catctttccg ctcattaaag tcccggacct tacgcaggac
     3661 gatctatcgc agtctggcct cctggcccca cctcatcaac gctatggcta tccattccca
     3721 agttgaaata ccggtgccgc gctaccccca cagtgtgcgg cgtcggcagg acggcgcgag
     3781 ttagttggtc cttctcgcgc cgtccgtttt acttcagcaa catctgtgcc ggtcacgtcg
     3841 ccaggccgag cggcccgcta cctgcgatgc cgaagatcaa cccgatcgtc atgtcgcgag
     3901 gaccgcacgg cctcctcagg accatctctg gtcgttcgcg gactgacagc ttgtatacga
     3961 taatgtcgaa aagtagccgc tcagcatttg caatcaatcg cgatcgagaa accctctagt
     4021 agatttgtgc actgcaacgt aaagccgcga tcatgaggat agcgatcatc gacaccagcg
     4081 ccggacgagc ggccgtcata tctgacggtt tgcgcgaggc cggtctcgag gatctcgttc
     4141 tggtggacac agccagacca cttgtcgccc aaattgaggc ggcgacaccc gacgtcgtac
     4201 ttgtcaacct ggaaaacccg agccgtgacc tgctcgagga tttcttcgcc atgtcgcgcg
     4261 cgctcgcgcg gccgatcgcg atgttcgtcg accagagcga cgccgaggcg accggggccg
     4321 cgattgacgc cggggtgtct gcctatgtcg tcgacggtct ttccaaacaa cgcatcaagc
     4381 cggtgatcga ccttgcgatc cggcgcttcc aggccttctc gaccctccag cgcgaactcg
     4441 acgaggcgaa gaacgccctc gctgaacgcg cgacgatcga ccgcgcgaag gcgatattga
     4501 tgaaacggcg gcaaattgac gaaccggccg cctacgcgct gctccgcggc caagcgatgc
     4561 gaaccaatcg gcggatcggc gaaatcgccg aggccatcgt cacgagcgaa gcgctgctgg
     4621 gagatatgga atgaccgaga gcttccgcat cggcttcctg ccgctcgtcg acgccgctct
     4681 ccccattctc gcgcacgaac tcggcttcgc ggcgcgcgag ggcatcgcga tcgaactggt
     4741 gcgcgacatg acctgggcaa cggtgcgcga ccggctgctc tatggtcaca ccgacgccgc
     4801 acacatgatc gcgccgctgg cgatcgcgac gccgctcggc cgcgaccggc cgccggtcgc
     4861 gctggcagtg cccttcgtgc tcgggctcaa cggcaatgcc gtgaccttct cgacgcgact
     4921 cgcccgcgag gtcggcctcg gcga
//
LOCUS       DATMSU010000286         8444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_71305, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000286 DATMSU010000000
VERSION     DATMSU010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71305"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VN034_07815"
     CDS             complement(<1..732)
                     /locus_tag="VN034_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWV60543.1"
                     /translation="MGRQPSAKPTSFDIAYLAGVSQPTVSRALRGSKSVSAATRANIE
                     RIARELNYTVDKNASSLRSQRTHTLALLFFEDPTPDESMINPFFLSMLGSITRQCALK
                     GYDLLISFQQMHNDWHVAYQDSHRSDGIILLGYGDYQLYRAKLEHLVEMDTKFVRWGS
                     VSDDSIGLTVGSDNVGAGELAGAHLVEIGRRHIAFLGDASDHAPEFQDRYTGLCRAMR
                     AAGIAPNPGLQAGAVSSEASGYAAAR"
     gene            1055..3805
                     /locus_tag="VN034_07820"
     CDS             1055..3805
                     /locus_tag="VN034_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60544.1"
                     /translation="MTMPTKFRRGASAIALGLALSVFVPGAAMAQDADTAPVDAAPAE
                     DAIVVTGIRGAINNSVQAKKTNTSIVEVISAEDIGKLPDLSIAESLSRLPGLATQRLD
                     GRANVVSIRGLAPDFTTTLLNGREQVSASNNRGVELDQYPSELLNGAVVYKTPDASLI
                     GQALGGTIDMRTVRPLAYGRRAIAAGARFEVNDLGKLNPDISNKGYRANISYIDQNAD
                     GTLGWAIGYARMQSPTAEERFNAWGYPDQDPGPDEVFLIGGAKPYVKSNELKRDGVMA
                     VVEWQPSDRLHMTIDGYWSKFKDEQRLRGIEFPLAWGNSTLQPGFTVEDGLVTEGVWT
                     GTEAVMRNDVVHRDSTIIAGGWNTKFKPNERLTLELDLGYSRLKKTEENLEIYLGTGR
                     GAGVGARETALGFEMRPNGGILFDPSLDYADPNLFVITDPQGWNSCGGAVPNCQDGFV
                     NTPKIKDELKSLRLQATQELDGVISSLRVGANYSDRKKSLDDRGFVLTSENYPANTPV
                     PADYLYDPVSLDFIGIPGMVAFDSWRFYNDGNYTLTDGAGFDPARVFNDYTIREKVLT
                     GFVQANFDADAGSAPVRGNVGVQIVHSDQTGSSFYAQVVGGVTQSTPITDGDTYTDIL
                     PSLNLSVEFADNTFLRFGAARMLARARMDQLKPGGGVNFDTSKRNNTDVNASPWSLDL
                     GNAKLRPLMADTVDVGLEKYFGQGGYVSIGGFYKYLENYIYRQVNPFDFSDFEVPDGG
                     TVGTRAGLSKQWLNGNAGRVYGAEASFSLPLANLTQSLDGFGLLGSASYTKSRVREGT
                     ENAISMPGLSKWVVNGTAYFEKAGFQVRASGRYRSKFLAEVSTISLARDMFMAKSEFV
                     VDAQVGYTFQSGALEGLGILLQASNLTNEPFVTYYNNDPRQIRDYQNYGRNFMAGVTY
                     KF"
     gene            3872..5380
                     /locus_tag="VN034_07825"
     CDS             3872..5380
                     /locus_tag="VN034_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HWV60545.1"
                     /translation="MGDSRITKVVIVGGGTAGWMAAAALSRTMDHLSIRLVESDEIGT
                     VGVGEATIPAIRLFNTLIGIDEDEFVRETRGTFKLGIQFHDWGRIGDTYMHAFGQIGR
                     SLGMLPFQQYWLRGRNEGVAGRLGDYSLNERTARQNRFARLPQIPNTSLDGIAYAFHF
                     DAALYAAYLRKVAEGAGVERTEGKIASVRRNGESGHVEAVVLENGTAIDGELFIDCSG
                     FRALLIEKALETGFEDWTHWLPCDRAIAVPSENAGPARPYTQAIAHKAGWQWRIPLQH
                     RTGNGHVFCSDFISEDEATATLLAHVEGRPLAEPRTLRFRTGMRKQAWNFNVVALGLA
                     SGFLEPLESTSIHLIQNGIAKLLAHFPGRDFDAANIAAYNRRVRFDYERIRDFIILHY
                     HANQRTDSPFWIRCREMGIPETLAHKIELFRSQGQIVREADELFVEIGWFQVLTGQNV
                     EPKGYHPMADQLTREDLAGFFADIETIVARTAAHLPTHEDFIAQHCAAGVPA"
     gene            5377..7170
                     /locus_tag="VN034_07830"
     CDS             5377..7170
                     /locus_tag="VN034_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWV60546.1"
                     /translation="MSLFASLIALSLSGAAPAADYRQRPPEDEIIYFVLPDRFENGDP
                     KNDRGGLKGDRLATGYDPSAKGFFHGGDLAGLTRRLDYIEGLGATAIWFAPIFKNKPV
                     QGPKGEESAGYHGYWVTDFTQVDPHFGTNEEFKAFVDAAHARGMKVYMDIIANHTADV
                     ITYKEDAADGFRYRSLADYPYSRRGGVKGAPVNPGFAGDHVADAGNWKKLADPAFAYT
                     PVVPKGEEKVKVPAWLNDPIYYHNRGNSDWVGESALYGDFSGLDDLATEHPRVVQGFI
                     DIYGRWIDEFGIDGFRIDTAKHVNPEFWQAFVPAMQARAKARGIPNFHIFGEVYVDAV
                     DPGALAWYTHAAGLPAVLDFGFARAAIDAVSGAKGTDQFAKLFDGDALYKGGTGTALG
                     LPTFLGNHDMGRFSMFVKAANGQASEAELLQRVMLGHSLLLTLRGVPTIYYGDEQGFV
                     SDDKDQLAREDMFASQVAAYNDNDLIGSDATTAAANFDPAHPLYRHIAALAEIRRKTP
                     ALTRGATKVRAFSDKPGLLAVSRFDPETGREVLLAFNTSAAPLAANIAIDMQSAGFTA
                     LHGDCPAKPAAPGSLHLSLPAFGTIICQASE"
     gene            7219..>8444
                     /locus_tag="VN034_07835"
     CDS             7219..>8444
                     /locus_tag="VN034_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWV60547.1"
                     /translation="MTAAATTPWWKGAVIYQVYPRSFADSNGDGIGDLAGITARLDYI
                     ASLGVDAIWLSPFYPSPMDDFGYDIADYCDVDPIFGTLADFDALVARAHALGLKVTTD
                     LVFAHTSDRHAWFAESRASRDNEKADWYVWADAKADGSPPTNWQSVFGGPAWTWDARR
                     GQYYLHNFLGSQPQLNVHNRDVQDALLGAVRFWLDRGVDGFRIDAINFSMHDPELRDN
                     PPAPPSNKPRTRPFDFQQKIYNQSHPDIPLFLERIRALTDQYPGRFTVAEVGGDDAVR
                     EMKLFTAGDRRLSSAYGFDFLYADRLTPQLVREAAEQWPDAPGVGWPSWAFENHDAPR
                     ALSRWTPEGVDAAAYARMKMALLCALRGNIILYNGEELGLDQVEIPFELVKDPEALKN
                     WPLTLSRDGARTPLPWA"
BASE COUNT         1530 a         2796 c         2656 g         1462 t
ORIGIN      
        1 ccgcgcggcc gcatagcccg acgcttccga cgacacggcg cccgcttgca accccgggtt
       61 aggcgcgatc cccgcggcgc gcatcgcgcg gcaaagcccg gtatagcggt cctgaaattc
      121 gggggcatgg tccgacgcgt cgccgaggaa ggcgatatgg cgccgcccga tctcgaccag
      181 atgcgcgccc gccagctcgc ccgcgccgac attgtccgac ccgacggtca gcccgatgct
      241 gtcgtccgaa accgatcccc agcgcacgaa tttcgtgtcc atctcgacca gatgttcgag
      301 cttggcgcga tagagctgat aatcgccata acccaaaagg ataatgccgt ccgaccggtg
      361 gctgtcctga taggcgacgt gccagtcgtt gtgcatctgc tggaaactga tgagcaggtc
      421 atagcccttg agcgcgcatt gccgcgtgat cgaccccagc atcgacagga agaaggggtt
      481 gatcatcgat tcgtcggggg tcggatcctc gaaaaagagc aaagcgagcg tgtgggtgcg
      541 ctgcgaacgc aggctcgagg cgttcttgtc gacggtgtag ttgagctcgc gcgcgatgcg
      601 ttcgatgttg gcgcgcgtcg cggcgctcac cgatttgctg ccgcgcagcg cgcgcgacac
      661 cgtgggctgc gagacgcccg ccagataggc gatgtcgaaa ctcgtcggct tggcggaggg
      721 ctggcgcccc atgtctctct ccctggcgcc gcggcccatt gtcgatggcc gccgctgacg
      781 tgcggagact agcgattggt cccctcgatg ccaagaattt gcaccgtgcc gatacccgcg
      841 gtttccggcg ccgaaagcgt tgcatttctg cgacagtacg cccccctgca tattatgcgt
      901 atacgtatac cctatggcgg cacgccgcga ttggtttcaa ttgcgaggct caagatggga
      961 aagcccatgc atcggagagg agcctgacgg acgccccgcg tccccggtcc gatgcacggg
     1021 tcaagaccac ccgtccgtgc caggggagcc acatatgacc atgccgacca agtttcgccg
     1081 gggtgccagc gccattgcgc tcggcctcgc cctgtccgtc ttcgtgcccg gcgccgcgat
     1141 ggcgcaggat gcggacaccg cgccggtcga tgcggcaccg gccgaagacg ccatcgtcgt
     1201 caccggcatt cgcggcgcga tcaacaattc ggtccaggcc aaaaagacga acacctcgat
     1261 cgtcgaagtg atttcggccg aggacatcgg caagctgccc gacctgtcga tcgccgaatc
     1321 gctgtcgcgc ctgccgggcc ttgcgacgca gcgcctcgac ggccgcgcca acgtcgtctc
     1381 gatccgcggt ctcgcccccg atttcaccac gaccttgctc aacggccgcg aacaggtttc
     1441 ggcgagcaac aaccgcggcg tcgaactcga ccaatatcct tcagagcttt tgaacggcgc
     1501 ggtcgtctac aagacccccg atgcgtcgct gatcgggcag gcgctcggcg gcacgatcga
     1561 catgcggacg gtgcgtccgc tcgcttatgg ccggcgcgcg atcgcggcgg gcgcgcggtt
     1621 cgaggtcaac gatctcggca aactcaatcc cgatatttcg aacaagggct atcgcgccaa
     1681 catctcttac atcgaccaga atgccgacgg cacgctcggc tgggcgatcg gctatgcgcg
     1741 catgcagtcg ccgaccgccg aggaacggtt caacgcctgg ggttatcccg accaggatcc
     1801 cggccccgac gaggtcttcc tgatcggcgg cgccaagccc tatgtgaagt cgaacgagct
     1861 gaagcgcgac ggcgtgatgg cggtcgtcga atggcagccg agcgaccggc tgcacatgac
     1921 gatcgacggc tattggtcga agttcaagga cgagcagcgg ctgcgcggca tcgagttccc
     1981 gctcgcatgg ggcaattcga cgctgcagcc cggcttcacc gtcgaagacg gtctggtgac
     2041 cgagggcgtc tggacgggca ccgaagcggt gatgcgcaac gatgtcgtcc accgcgactc
     2101 gacgatcatc gcgggcggct ggaacaccaa gttcaagccg aatgagcggc tgacgctcga
     2161 actcgatctc ggctattcgc ggctcaagaa gaccgaagaa aatctcgaaa tctatctcgg
     2221 caccgggcgc ggtgcgggcg tcggcgcgcg cgagaccgcg ctgggcttcg agatgcgccc
     2281 gaacggcggc atcctgttcg acccatcgct cgactatgcc gaccccaatc tgttcgtgat
     2341 caccgacccg cagggctgga acagctgcgg cggcgcggtt cccaattgtc aggacggctt
     2401 cgtcaacacg ccgaagatca aggacgagct gaaatcgctg cgccttcagg cgacgcagga
     2461 actggacggc gtgatcagca gccttcgcgt cggggccaat tattcggacc gcaagaagag
     2521 cctcgacgac cgcggcttcg tgctgaccag cgaaaattat ccggccaaca cgccggtgcc
     2581 cgccgattat ctctatgacc ccgtgtcgct cgatttcatc ggcattcccg gcatggtcgc
     2641 attcgacagc tggcgcttct acaatgacgg caattatacg ctgaccgatg gcgcgggttt
     2701 cgatcccgcg cgcgtcttca acgactatac gatccgcgaa aaggtgctga ccggcttcgt
     2761 ccaggcgaat ttcgacgccg atgcgggcag cgcgccggtg cgcggcaatg tcggcgtcca
     2821 gatcgtccat tcggaccaga cggggtcgag cttctatgcg caggtcgtcg gcggcgtgac
     2881 ccagtcgacg ccgatcaccg acggcgacac ctacaccgac atcctgccga gcctgaacct
     2941 gtcggtcgaa ttcgccgaca atactttcct gcgcttcggc gcggcgcgga tgctggcgcg
     3001 ggcgcgcatg gatcagctca agcccggcgg cggcgtcaac ttcgacacgt cgaaacgcaa
     3061 caacaccgac gtcaacgcct cgccctggtc gctcgacctc ggcaatgcga agctgcgtcc
     3121 gctgatggcc gacacggtcg atgtcgggct cgagaaatac ttcggccagg gcggctatgt
     3181 ctcgatcggc ggcttctaca aatatctcga aaactacatc tatcgtcagg tcaatccgtt
     3241 cgacttctcg gacttcgagg ttcccgatgg cggaacggtc ggcacccgcg cggggctcag
     3301 caagcagtgg ctgaacggca acgccggccg cgtctatggc gccgaggcgt ccttctcgct
     3361 gcccttggca aacctgaccc aatcgctcga cggcttcggc ttgctcggca gcgcctccta
     3421 taccaagagc cgcgtgcgcg aaggcacgga gaacgcgatt tcgatgcccg gcctgtcgaa
     3481 atgggtcgtg aacggaaccg cctatttcga aaaggcgggg ttccaggttc gcgcatcggg
     3541 ccgctatcgt tcgaaattcc ttgcggaagt ttcgacgatc agtctcgcgc gtgacatgtt
     3601 catggcgaag agcgagttcg tcgtcgatgc gcaggtcggc tataccttcc agagtggtgc
     3661 gctcgaaggg ctcggcatcc tgctccaggc gtcgaacctc accaacgagc cgttcgtgac
     3721 ctattacaac aacgacccgc gccagatccg cgactatcag aattatggtc gcaacttcat
     3781 ggccggtgtc acctacaagt tctgatccga tgcgcgggcc ggcacgtccg gcccgcttgt
     3841 tgcaggtgcc ggacgagagg gaaggaacag cgtgggcgac agtcgcatca cgaaggtggt
     3901 gatcgtcggt ggcggcaccg cgggctggat ggccgccgcg gcgctgtcgc gcacgatgga
     3961 tcatctgtcg atccggctgg tcgagagcga cgaaatcggc accgtcggcg tcggcgaggc
     4021 gacgatcccg gcgatccgcc tgttcaatac tctcatcggc atcgacgagg atgaattcgt
     4081 ccgcgagacg cgggggacgt tcaagctcgg tatccagttc cacgactggg gccggatcgg
     4141 cgacacctat atgcacgcct tcgggcagat cgggcgcagc cttggcatgc tgccgttcca
     4201 gcaatattgg ctccgcggcc ggaacgaagg cgtcgcgggc cggcttggcg attattcgct
     4261 gaacgaaagg acggcgcggc agaaccgctt tgcgcggctg ccgcagatcc cgaacacaag
     4321 cctcgacggc atcgcttacg cctttcattt cgacgcggcg ctttatgccg cctatctgcg
     4381 caaggtcgcc gaaggcgcgg gggttgagcg gacggagggc aagatcgcca gcgtccggcg
     4441 gaacggcgaa agcgggcatg tcgaagcggt cgtgctggaa aatggcaccg cgatcgacgg
     4501 cgagcttttc atcgactgct cgggctttcg cgcgctgctg atcgagaagg cgctcgaaac
     4561 cggcttcgag gattggacgc actggcttcc ctgcgatcgc gcgatcgcgg tgccgagcga
     4621 aaacgccggt ccggcgcggc cctatacgca ggcgatcgcg cacaaggccg gctggcagtg
     4681 gcgcatcccg ctccagcacc gcaccggcaa cggtcatgtc ttttgcagcg acttcatcag
     4741 cgaggacgag gcgaccgcga ccctgctcgc ccatgtcgag gggcgtccgc tcgccgaacc
     4801 gcggacgctg cgtttccgta ccgggatgcg aaagcaggcg tggaacttca atgtcgttgc
     4861 gctcgggctc gcctcgggtt tcctcgaacc gctcgaatcg acgagcatcc acctgatcca
     4921 gaacggcatc gccaagctgc tcgcgcattt tccgggccgc gatttcgatg ccgccaatat
     4981 cgcggcctat aaccgccgcg ttcgcttcga ttatgagcgt atccgcgact tcatcatcct
     5041 ccactatcac gccaatcagc gcaccgactc gcccttctgg atccgctgcc gcgagatggg
     5101 cattcccgaa acgctcgcgc acaagatcga gctgttccgc agtcaggggc agatcgtccg
     5161 cgaagccgac gaactgttcg tcgaaatcgg ctggtttcag gtgctgacgg ggcagaatgt
     5221 cgagccgaag ggctatcatc cgatggccga ccagctcacg cgtgaggacc tcgcgggctt
     5281 tttcgccgac atcgaaacga tcgtcgcgcg caccgcggcg catctgccga cccacgaaga
     5341 tttcatcgcg cagcattgcg cggcaggagt gcccgcatga gcctgttcgc atccttgatc
     5401 gcgctaagcc tgtcgggcgc cgcgcccgcc gccgattacc gccagcggcc gcccgaggat
     5461 gagatcatct atttcgtcct tcccgaccgc ttcgaaaatg gcgatccgaa gaacgaccgc
     5521 ggagggctga agggcgaccg gctcgcgacg ggctatgacc caagcgccaa gggctttttc
     5581 cacggcggcg acctcgcggg cctcacccgt cggctcgact atatcgaggg actgggcgcc
     5641 accgcgatct ggttcgcgcc gatcttcaag aacaagccgg tccagggccc gaagggcgag
     5701 gaaagcgcgg gctatcacgg ctattgggtg accgatttca cgcaggtcga cccgcatttc
     5761 ggcaccaatg aagagttcaa ggccttcgtc gacgccgcgc atgcgcgcgg gatgaaggtc
     5821 tatatggaca tcatcgccaa ccacaccgcc gacgtgatca cctacaagga ggacgcggct
     5881 gacggcttcc gctaccgcag cctcgccgat tatccttatt cgcggcgggg cggggtgaag
     5941 ggcgcgccgg tcaacccggg tttcgcgggc gatcatgtcg ccgatgccgg caactggaaa
     6001 aaactggccg acccggcttt cgcctacacc cccgtcgtgc cgaagggcga ggagaaggtg
     6061 aaggtgcccg cctggctcaa cgacccgatc tattatcaca accgcggtaa cagcgactgg
     6121 gtcggagagt ccgcgctcta cggcgatttt tcggggctcg acgatctcgc gaccgaacat
     6181 ccccgcgtcg tgcagggctt catcgacatc tacggccgct ggatcgacga attcggcatc
     6241 gacggcttcc gcatcgacac cgccaagcat gtgaaccccg aattctggca ggcgttcgtt
     6301 cccgcgatgc aggcgcgggc gaaggcgcgc ggcatcccca atttccacat cttcggcgag
     6361 gtctatgtcg acgccgtcga tcccggcgcg ctcgcctggt acacgcatgc ggcggggctg
     6421 cccgcggtgc tcgacttcgg cttcgcgcgc gcggcgatcg atgccgtgag cggggcgaag
     6481 gggaccgacc agttcgccaa gctgttcgac ggcgacgcgc tctacaaggg cggtaccggc
     6541 actgcgctcg gtctcccgac cttcctcggc aaccacgaca tgggtcgctt ttcgatgttc
     6601 gtgaaggccg cgaacgggca agcgagcgag gccgaactgc tgcagcgcgt gatgctcggc
     6661 cacagcctgt tgctgacgct gcgcggcgtg ccgacgatct attatggcga cgagcagggg
     6721 ttcgtttcgg acgacaagga ccagctcgcg cgcgaggata tgttcgcgtc acaggtcgcc
     6781 gcctataacg acaacgacct gatcggcagc gatgcgacga ccgcggccgc caatttcgac
     6841 cccgcgcacc cgctctatcg ccatatcgcg gcgctcgccg aaatccggcg caagacaccc
     6901 gcgctcaccc gcggcgcgac gaaggtccgc gctttctccg acaagcccgg cctgctcgcg
     6961 gtatcgcgct tcgaccccga aaccgggcgc gaagtcctgc tcgccttcaa tacctcggcg
     7021 gcgccgctgg cggcgaatat tgccatcgat atgcaaagcg cgggctttac cgcgctccat
     7081 ggcgattgtc cggcaaagcc cgccgcgccg ggcagcctcc acctttctct ccccgccttc
     7141 ggcacgatca tctgccaggc cagcgaataa gcgaaagcac gcgtgaccac gaacagcccc
     7201 ctcgttcagg accagaccat gaccgccgcg gcgaccacgc catggtggaa gggcgcggtg
     7261 atctatcagg tctatccgcg cagcttcgcc gactcgaacg gcgacggcat cggcgacctt
     7321 gccggcatca ccgcacggct cgattatatc gcttcgctcg gtgtcgacgc gatctggctg
     7381 tcgcccttct acccctcgcc gatggacgat ttcggctatg acatcgccga ttattgcgat
     7441 gtcgacccga tcttcggcac gctcgccgat ttcgacgcgc tggtggcgcg cgcgcatgcg
     7501 ctggggctga aggtcacgac cgacctcgtc ttcgcgcaca cctccgaccg gcacgcgtgg
     7561 ttcgcggaaa gccgcgcgag ccgcgacaat gaaaaggccg actggtatgt ctgggccgat
     7621 gccaaggccg acggttcgcc gccgaccaac tggcagtcgg tgttcggcgg ccccgcctgg
     7681 acatgggacg cgcggcgcgg ccaatattat ctgcacaatt tcctcggctc gcagccgcag
     7741 ctcaacgtcc acaaccgcga cgtgcaggac gcgctgctcg gcgcagtgcg cttctggcta
     7801 gaccgcggcg tcgacggctt ccgcatcgat gcgatcaatt tttcgatgca cgaccctgag
     7861 cttcgcgaca atccgccggc gccgccgtcg aacaagccgc gcacccggcc gttcgatttc
     7921 cagcagaaaa tctacaacca gtcgcacccc gacattccgc tgttcctcga acgtatccgc
     7981 gcgctgaccg atcaatatcc gggccgcttc accgtcgccg aagtcggcgg cgacgatgcg
     8041 gtgcgcgaga tgaagctgtt caccgcgggc gaccgccggc tgagcagcgc ctatggcttc
     8101 gacttcctct atgccgaccg gctgaccccg caactcgtgc gcgaggcggc cgagcagtgg
     8161 cccgatgcgc cgggggtggg ctggccgagc tgggcgttcg aaaatcacga tgcgccgcgc
     8221 gcgctgtcgc gctggacccc cgaaggcgtc gatgcggcag cctatgcgcg gatgaagatg
     8281 gcgctgctct gcgcgctgcg cggcaatatc atcctctata atggcgagga gctcgggctc
     8341 gatcaggtcg agattccgtt cgaactcgtc aaggaccccg aggcgctcaa gaactggccg
     8401 ctcaccctgt cacgcgacgg cgcgcggacg ccgctgccgt gggc
//
LOCUS       DATMSU010000287         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_23784, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000287 DATMSU010000000
VERSION     DATMSU010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23784"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1535)
                     /locus_tag="VN034_07840"
     CDS             complement(33..1535)
                     /locus_tag="VN034_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWV60548.1"
                     /translation="MRYVYGITSALLAGGTALALVTQSPVGAQVAQNEKSEIAKAVPR
                     AGAPESFADLVEQLQPAVVNISTKQEVTLGVRLNPFAGTREPITQEQQGGGSGFLISA
                     DGYIVTNNHVIAAGRPGEQKTVDQVTVTLTNQKEYTAKIVGRDVASDLALLKIDASGL
                     PFVKFADGSPARVGDWVVAIGNPLGLGSTVTAGIISAVQRNIGQGGAYDRYIQTDTAI
                     NRGNSGGPLFDLQGNVVGINNMLISPVGANIGVNFAIPADAAIPVIEALRAGEAPQRG
                     YLGIGIVPVSEDMAAALGLPKDRGEIVQRIEDGQAAAKAGLKRGDVVTKINGKEITPQ
                     QTLSYIVANIKPGTRVPIEVIREGKTLSLSAVIGTRPPESELTGEDFDPEAEQTLPED
                     PTGTADQAIQDQLGMAVQPLTTAIARSIGVEADTKGLVIAAVGGNSDAGRKGLRRGDI
                     ILSANRSAVTSSEALSGVLAAAKKAGRNAVLLEILRRGQPPAFIAIRIAE"
     gene            complement(1676..2527)
                     /locus_tag="VN034_07845"
     CDS             complement(1676..2527)
                     /locus_tag="VN034_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWV60549.1"
                     /translation="MFASLFRNPIRLLVVVVALLVILSQSLAIVPEDKQALILRFGEI
                     ERTVNRYKPNEDFGRSGAGLVLRVPFTDGIQYIDKRILGVNMERQQVLSTDQQRLQVD
                     AFARFRITNPVRMYTAIRTEDKLQTQLATILGSSLRNELGKRTFATLLSPERGAVMDN
                     IQVALNREAQKYGAEIIDVRIKRADLPEGATLEAAYNRMRTARQQEAISIRAEGQKEA
                     QIIRGAADGEAARIYAASFGKDPEFYDFYRAMQSYRQTFLGENNQGGTSIIMSPDNEY
                     LERFSGG"
     gene            complement(2527..3696)
                     /locus_tag="VN034_07850"
     CDS             complement(2527..3696)
                     /locus_tag="VN034_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HWV60550.1"
                     /translation="MSNENDANMAGRRPGGLRQFLQQISQMANSPWGNGPKDGDDGGK
                     GSGGDGKRPGPRNPWVTPDPADQRRGPKPRGPSALDELLRKGRGGFGGGGSGGSGGQF
                     NLGDSAKFWKWAVLAVVVAWVVFSSFHIVPPEKEGVVTRLGSYSRTVGPGVKFTWPAP
                     IERIRMEDVRAIRTMPVGSPNATDENFVLTRDQSIVDLAYEVRWSVRDPELFFFQLAD
                     PEGTIREVAESSMRATVANFDLVQAIGPGRVEIEAQVQQRMQELLDQYRAGVLIQGIA
                     IRQVDPPSQVDEAFKEVTAARQERESAINLARAYEQQVLERARGDTSAFDQIYAQYQL
                     APEVTRQRLYYETMETVLSNVDKTIIEARGVTPYLPLNEVQKRLKAPDAAQKGGE"
     gene            3788..>4311
                     /locus_tag="VN034_07855"
     CDS             3788..>4311
                     /locus_tag="VN034_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HWV60551.1"
                     /translation="MTDTAPLASIAADLSGGRTSGLRFLDDKGTLAIVLDVTGIAAEE
                     RGPLEDRLRAGLLEEPDVNEVRIAMTAEKKAMTIIAVGSGKGGVGKSTLAANLAVALR
                     RLGVKVGLVDADIYGPSQPRLMDSEGVKPESRGSKLVPVANAYGVPMLSTGQIAAPGQ
                     AIAWRGPMAGKALE"
BASE COUNT          664 a         1411 c         1454 g          782 t
ORIGIN      
        1 gcggcgccct ttccggttcg ggtcgcgccc gcctattcgg cgatgcggat cgcgatgaac
       61 gccggcggct ggccgcggcg caggatttcg agcagcaccg cgttgcggcc cgccttcttc
      121 gccgccgcga ggacacccga gagcgcctcg ctgctcgtca ccgcgctgcg gttggcgctg
      181 aggatgatgt cgccgcggcg caatcccttg cggcccgcgt cgctgttgcc gccgacggcg
      241 gcgatcacca gccccttggt gtcggcctcg acgccgatcg agcgcgcgat ggcggtcgtc
      301 agcggctgga ccgccatgcc gagctggtcc tggatcgctt gatcggcggt gcccgtcgga
      361 tcctcgggca gcgtctgttc ggcttcggga tcgaaatcct cgccggtcag ctcgctttcg
      421 ggcgggcggg tgccgatgac ggccgacagc gacagcgtct tgccttcgcg gatgacctcg
      481 atcgggacgc gcgtgcccgg tttgatgttg gcgacgatat aggacagcgt ctgctgcggc
      541 gtgatttcct tgccgttgat cttggtcacg acgtcgccgc gcttcagccc ggccttcgcc
      601 gcggcctggc cgtcctcgat acgctggacg atctcgccgc gatccttggg caggccgagc
      661 gccgccgcca tatcctcgct gaccggcacg atgccgatac cgagatagcc gcgctggggc
      721 gcttcgccgg cgcgcagcgc ctcgatcacc ggaatcgcgg cgtcggcggg aatcgcgaaa
      781 ttgacgccga tgttcgcgcc gacgggcgag atcagcatat tgttgatgcc gacgacattg
      841 ccctgcaggt cgaacagcgg gccgcccgaa ttgccgcggt tgatcgcggt atcggtctgg
      901 atatagcggt cataggcgcc gccctggccg atattgcgct ggaccgccga gatgatcccc
      961 gcggtcacgg tcgagccgag cccgagcggg ttgccgatcg cgacgaccca gtcgccgacg
     1021 cgcgccgggc tgccgtcggc gaacttcacg aagggcaggc ccgacgcgtc gatcttgagc
     1081 agcgcgaggt ccgacgcgac gtcgcggccg acgatcttcg ccgtatattc cttctggttg
     1141 gtgagcgtga cggtgacctg atcgacggtc ttctgctcgc cgggccggcc ggcggcgatc
     1201 acatggttgt tggtgacgat atagccgtcg gccgagatca ggaagcccga gccgccgccc
     1261 tgctgttcct gcgtgatcgg ctcgcgcgtt ccggcaaagg gattgagccg gacgccgagc
     1321 gtcacttcct gcttggtcga gatgttgacc accgcgggct gcaactgctc gacgaggtcg
     1381 gcaaagcttt cgggcgcgcc ggcgcgcgga acggccttgg cgatctcgct cttttcgttc
     1441 tgcgcgacct gcgcgccgac gggggactgg gtgaccagcg ccagcgcggt gccacccgcc
     1501 agcaaggccg aagtgatgcc ataaacataa cgcacgatcg gaaatcctct tcgttcttca
     1561 aaaaatccaa taaaccccgg ctgcggcggt ctgcggcggg ccggctgaac gctgtttgaa
     1621 catgaacgga cgctgtcggt tccattcatc gcgccggccg cccaccgcga acgcgtcaac
     1681 cgccgctgaa acgctccaga tattcattgt cgggcgacat gatgatcgac gttccaccct
     1741 ggttgttttc acccaggaag gtctgccgat agctttgcat cgcgcgatag aaatcataga
     1801 attcggggtc cttgccaaag ctcgcggcat agatgcgcgc ggcttcgccg tcggcggcgc
     1861 cgcggatgat ctgcgcttcc ttctgccctt cggcgcgaat cgagatcgct tcctgctgcc
     1921 gcgcggtgcg catgcgatta tacgccgctt cgagcgtcgc gccttcgggc aggtcggcgc
     1981 gtttgatccg cacgtcgatg atctcggcgc catatttctg cgcctcgcga ttgagcgcga
     2041 cctggatatt gtccatcacc gcgccgcgct cgggcgacag caaagtcgcg aaggtgcgct
     2101 tgccgagctc gttgcgcagc gacgagccga ggatcgtcgc gagctgcgtc tgcaacttat
     2161 cttcggtgcg gatcgcggtg tacattcgca ccggattggt gatgcggaag cgcgcgaagg
     2221 cgtcgacctg cagccgctgc tggtcggtgg acaatacttg ctgacgctcc atgttcacgc
     2281 cgaggatgcg cttgtcgata tattggatgc cgtcggtgaa agggacgcgc aggacgagcc
     2341 cggcacccga gcgcccgaaa tcctcgttcg gcttgtagcg gttcaccgtg cgttcgatct
     2401 cgccgaagcg caggatcagc gcctgcttgt cctcgggcac gatcgccagc gattgcgaca
     2461 ggatgacgag cagcgcgacg acgacgacga gcaggcggat cgggttccgg aacagggagg
     2521 cgaacatcat tcgcctccct tctgggcggc atcgggcgcc ttcagccgct tctgcacttc
     2581 gttgagcggc agataggggg tcacgccgcg cgcttcgatt atcgtcttgt cgacattcga
     2641 caacaccgtc tccatcgttt cataataaag gcgctggcgc gtcacttcgg gggccagctg
     2701 atattgggcg taaatctggt cgaacgccga ggtgtcgccg cgcgcgcgct cgagcacctg
     2761 ctgttcatag gcgcgcgcga ggttgatcgc cgattcgcgt tcctggcgcg ccgcggtgac
     2821 ttccttgaac gcctcgtcga cctggctcgg cggatcgacc tggcggatcg cgatgccctg
     2881 gatcagcacg cccgcgcgat actggtcgag cagttcctgc atgcgctgct ggacctgcgc
     2941 ctcgatctcg acgcggcccg ggccgatcgc ctggacgagg tcgaaattgg cgaccgtcgc
     3001 gcgcatcgag ctttcggcga cttcgcggat cgtcccttcg ggatcggcga gctggaagaa
     3061 gaacagctcg ggatcgcgca ccgaccagcg gacctcatag gcgaggtcga cgatgctctg
     3121 gtcgcgcgtc agcacgaaat tctcgtcagt cgcgttcggc gaaccgaccg gcatcgtgcg
     3181 gatcgcgcgg acgtcttcca tccggatgcg ctcgatcggc gccggccagg tgaatttgac
     3241 gcccggcccg acggtgcgcg aatagctgcc gaggcgcgtc accacgcctt ccttttcggg
     3301 ggggacgatg tggaaggagg aaaagaccac ccacgcgacg acaaccgcga ggacggccca
     3361 tttccagaac ttcgccgaat cgcccagatt gaactggccg ccgctgccgc ccgatccgcc
     3421 gccgccgaaa ccgccgcgtc ccttgcgcag cagctcgtcg agcgccgagg ggccgcgcgg
     3481 ttttggcccg cgccgctggt cggcgggatc gggggttacc caagggttgc gcggcccggg
     3541 gcgcttgccg tcgccgccac tgcccttccc gccatcgtcg ccgtctttgg ggccattgcc
     3601 ccacgggctg ttcgccatct ggctgatctg ctggagaaat tgccgcaatc cccccggacg
     3661 gcggccggcc atgttggcgt cattttcgtt gctcatgctg cttttatagg ggcggcgggc
     3721 gcgattaaca gggggtgcgc gcgaaaatgg ctggcaatcg gcttcgtcat gcctatctgc
     3781 cgcgagcatg accgacaccg ctcccctcgc cagcatcgcc gccgacctct ccggcggccg
     3841 cacttcgggc cttcgctttc tcgacgacaa ggggacgctc gcgatcgtcc tcgacgtgac
     3901 cgggatcgcc gcagaggagc gcggaccgct cgaggacagg ttgcgcgcgg gcctgctcga
     3961 agagccggac gtgaacgaag tgcgcatcgc gatgactgcc gagaaaaagg cgatgacgat
     4021 catcgcggtc ggcagcggca agggcggggt cggcaaatcg acgctcgccg cgaacctcgc
     4081 ggtcgcgctg cgccggctgg gggtcaaggt cgggctggtc gacgccgaca tctatggacc
     4141 gtcgcaaccc cggctgatgg acagcgaggg cgtgaagccc gaatcgcgcg gatcgaagct
     4201 ggttcccgtt gccaacgcct atggcgtgcc gatgctgtcg accgggcaga tcgcagcgcc
     4261 ggggcaggcg atcgcgtggc gcggcccgat ggcgggcaag gcgctcgaac a
//
LOCUS       DATMSU010000288         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_137679,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000288 DATMSU010000000
VERSION     DATMSU010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137679"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="VN034_07860"
     CDS             complement(<1..163)
                     /locus_tag="VN034_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWV60552.1"
                     /translation="MRIFAATPEARPRPHANGFTLVELMVVLAIMALAATAVVLTIPG
                     EERTVRSEAD"
     gene            complement(138..644)
                     /gene="gspG"
                     /locus_tag="VN034_07865"
     CDS             complement(138..644)
                     /gene="gspG"
                     /locus_tag="VN034_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HWV60553.1"
                     /translation="MSLMKLFLHLMLDTSFSDRGHPPVHRRRKKDERGFTLTELMVVI
                     FIIGLLATVVMINVLPSQDRAMVTKAKADIATLENALEQYRLDNLVYPASTDGLNALV
                     TAPPALAQPERYRRGGYIKKLPADPWGRPYNYQAPGPNGKAFDVWSLGADGAPGGTDD
                     NADIRSDA"
     gene            complement(647..1861)
                     /gene="gspF"
                     /locus_tag="VN034_07870"
     CDS             complement(647..1861)
                     /gene="gspF"
                     /locus_tag="VN034_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HWV60554.1"
                     /translation="MPDYRYVAIDPQGRERKGRLTAANDDAARADLIRRKFHIVAVEA
                     AGAKPAGRSLLAFRKAKLSSKELALFTRQLATLAEVAPLEEALRTLTRQSEAESARAI
                     IGDVHAGLLEGRRLADAMGRQPTSFPPLYRAMVAAGETTGSLTTILARLADLLERQAE
                     VRGKLIAALAYPIVLAIVAIGVVAALMIFVVPRVVEQFTDTGQQLPFLTRAVIAISSF
                     AANWWWLIALLIVAAAFGWTAALRRPAFKAAVDARLLRLPLLGRLLRDLYAARFARTL
                     STMVSSRLPLVEGLKLTVPTIRNAALAGATANIVDQVRAGGGLSAALRDAGVFPPLLV
                     YMTASGESAGRLEVMLERAADYLEREFDRFTAASMALLEPVIIVVMGSCVALIILAIL
                     LPILQLQNLAGL"
     gene            complement(1854..>2814)
                     /locus_tag="VN034_07875"
     CDS             complement(1854..>2814)
                     /locus_tag="VN034_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWV60555.1"
                     /translation="IAERRVPQDGRIGLTLAGKAVDVRVSTLPSRAGERVVMRILDKD
                     TAGIDFDVLGLSGEADRILREALAEPNGIILVTGPTGSGKTTTLYAALKQLNDGQRNI
                     LTVEDPVEYAVDGVGQTQVNSKVGLDFAAGLRAILRQDPDVVMVGEIRDRETADIAVQ
                     ASLTGHLVLSTVHTNDAVGAITRLKDLKVEPFLLASTLRAVLAQRLVRKLCENCREPV
                     QADNSIAAMLGLDIGTVIWRPKGCEACGGTGFKGRIGVFEAIKVDETVRRYIYAGGDE
                     AMIAKHAFLKSPTLASAARSMVAKGLTTAEEAIRIARREDVDA"
BASE COUNT          468 a          880 c          995 g          471 t
ORIGIN      
        1 ggtcggcttc gctgcgcacc gtgcgttcct cgcccgggat ggtcagcacg accgcggtcg
       61 cggcgagcgc catgatcgcg agcacgacca tcagttcgac gagggtgaag ccgttggcgt
      121 gaggacgcgg gcgcgcctca ggcgtcgctg cgaatatccg cattgtcgtc ggtcccgccg
      181 ggggcgccgt cggcaccgag cgaccagacg tcgaacgcct ttccgttcgg ccccggcgcc
      241 tgataattat aggggcgccc ccacggatcg gcgggcagtt tcttgatata gccgccgcgg
      301 cgatagcgtt cgggctgggc cagcgccgga ggcgcggtga cgagcgcgtt cagcccgtcg
      361 gtcgaggccg gatagaccag attgtcgagc cgatattgtt caagggcatt ttcgagcgtc
      421 gcgatatcgg ccttcgcctt ggtcaccatc gcccggtcct ggctcggcag gacgttgatc
      481 atcaccacgg ttgcgagcag cccgatgatg aagatgacga ccatgagttc ggtcagcgtg
      541 aagccgcgct cgtccttctt gcggcggcgg tgcacgggcg gatggccccg atccgaaaag
      601 gacgtatcga gcatcaggtg gaggaatagc ttcatcagcg acattttcat agtccggcta
      661 gattctgcaa ctgaaggatc ggaagcagaa tggcgaggat gataagggca acgcatgacc
      721 ccataacgac aattatgaca ggttcgagaa gcgccatcga cgccgccgta aagcggtcga
      781 attcgcgttc gagataatcg gcggcgcgtt cgagcatcac ttcgagccgc ccggcgcttt
      841 cgccgctcgc ggtcatatag acgagcagcg gcgggaagac gccggcgtcg cggagcgcgg
      901 ccgagagacc gccaccggcg cggacctgat cgacgatgtt ggcggtcgcg ccggcgagcg
      961 cggcgttgcg gatcgtcggc acggtgagct tgagcccttc gacgagcggc aagcggctgg
     1021 agaccatcgt cgacagcgtc cgcgcgaagc gcgccgcata gagatcgcga agcaggcgcc
     1081 cgagaagcgg caggcgaagc aggcgggcgt cgaccgcagc cttgaacgcc gggcggcgca
     1141 gcgcggcggt ccagccgaat gcggcggcga cgatcagcag cgcgatcagc caccaccaat
     1201 tggcggcgaa gctggagatg gcgatcaccg cgcgggtcag gaaaggcagt tgttggccgg
     1261 tgtcggtgaa ttgttcgacg acgcgcggga cgacgaagat catcaatgcc gcgacgacac
     1321 cgatcgcgac gatcgcgagc acgatcggat aggcgagcgc ggcgatcagc ttgccgcgca
     1381 cctcggcctg gcgttcgagc aggtcggcga ggcgcgcgag gatcgtcgtg aggctgcccg
     1441 tcgtttcgcc tgccgcgacc atcgcgcgat agagcggcgg aaagctcgtc ggctggcggc
     1501 ccatcgcgtc ggcgaggcgg cggccttcga gcaggcccgc atggacgtcg ccgatgatcg
     1561 cgcgcgcgct ttcggcctcg ctctgacgcg tcaatgtacg cagcgcctct tcgagcggcg
     1621 cgacttcagc gagcgtcgcg agttggcggg taaaaagcgc cagttccttg ctgctgagtt
     1681 tggccttgcg gaaggcgagc agcgagcggc cggcgggctt cgctccggcg gcttcgaccg
     1741 cgacgatgtg gaatttccgc cgtatcaggt cggcgcgcgc ggcatcgtcg ttcgcggcgg
     1801 tcagtcgtcc cttgcgctcg cgaccctggg ggtcgatcgc cacatagcga taatcaggca
     1861 tcgacgtctt cgcgccgcgc gatgcggatc gcctcctcgg cggtggtcag ccccttcgcg
     1921 accatcgacc gcgccgccga ggcgagtgtc ggcgatttga ggaaggcgtg tttggcgatc
     1981 atcgcttcgt cgccgccggc gtagatgtag cggcggacgg tctcgtcgac cttgatcgct
     2041 tcgaacacgc cgatgcggcc cttgaagccg gtgccgccgc acgcctcgca gcctttgggg
     2101 cgccagatca cggtgccgat gtcgagcccg agcatcgcgg cgatgctgtt gtcggcctgg
     2161 acgggttcgc ggcaattttc acaaagtttg cgcacaagtc gttgcgcaag aacggcgcgg
     2221 agggtcgagg ccagaagaaa aggctctact ttcaaatcct taagtcgcgt tattgcgccg
     2281 acggcatcat tggtgtggac ggtcgagagg acgaggtggc cggtgagcga ggcctgcacc
     2341 gcgatgtcgg cggtctcgcg gtcgcggatt tcgccgacca tcacgacgtc cggatcctgg
     2401 cgcaggatcg cgcggaggcc cgcggcgaag tcaagcccga ccttgctgtt cacctgcgtc
     2461 tggccgaccc cgtcgacggc atattcgacc ggatcctcga cggtcaggat attgcgctgg
     2521 ccgtcgttga gttgcttgag cgcggcgtag agcgtcgtcg tcttgcccga acccgtcggg
     2581 ccggtgacga ggatgatgcc attgggttcg gcaagcgcct cgcgcaggat gcggtcggcc
     2641 tcacccgaca ggccgaggac gtcgaagtcg atccccgccg tgtccttgtc gagaatacgc
     2701 atcaccaccc gctcgcccgc gcggctcggc aaggtcgaga cgcggacgtc gaccgccttg
     2761 cccgcgagcg tcagcccgat gcggccgtcc tgcggcacgc ggcgctcggc gatg
//
LOCUS       DATMSU010000289         4642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_90534, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000289 DATMSU010000000
VERSION     DATMSU010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90534"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VN034_07880"
     CDS             complement(<1..644)
                     /locus_tag="VN034_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HWV60556.1"
                     /translation="MFERDIDSENDGRRERIRFWSTIVLGAILTGVVGALVLLLARAN
                     DNYDRSLGWQTQSMEVISQTRSVDAAIARAEAALGRFAVGLQKEDGRIYEYQWGRARQ
                     FMTRLSRNVRDNPKQIALVEQLAREMDSRGEQLGDAALSANYNQTVAAISKFYAAGQG
                     DGLARIDNLLSEIIANERALLRERNRLAAADRASLNQAILLFSLLGAAAAVIAIG"
     gene            complement(747..1949)
                     /locus_tag="VN034_07885"
     CDS             complement(747..1949)
                     /locus_tag="VN034_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWV60557.1"
                     /translation="MTDLFSKFDPLIQQREALLATGVTDPFSLVMEKVVSPTVAMCNG
                     RETILLGTYNYMGMTFDEDVIAAGKDALDKFGSGTTGSRVLNGTYQGHKECEDALREF
                     YAMDHAMVFSTGYQANLGIISTIAGKGDYVILDIDSHASIYDGCKMGDAEIVAFRHND
                     IEALEKRLKRLPPDAGKLVVLEGVYSMLGDVAPLKEMVRIAKENGAMVLVDEAHSMGF
                     IGEHGRGVAEAQGVIDDVDFIIGTFSKSVGTVGGFCVSNHPKFEIMRLVCRPYVFTAS
                     LPPSVVATAATSIRKLMLGSNKRAHLWENSKMLHQGLKDLGFTLGTDEPQSAIIAVIM
                     PDLERGAMMWEALLEEGLYVNLARPPATPAGMTLLRCSLCAEHSSEQVGEILGRFERA
                     GKRTQIIG"
     gene            complement(1958..2194)
                     /locus_tag="VN034_07890"
     CDS             complement(1958..2194)
                     /locus_tag="VN034_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWV60558.1"
                     /translation="MSTAIKDQIYGLIAPFNKKGVELSDATTFAGDLEWDSLTVMDFV
                     AEVEDTFDIIISMNMQAEIENVGQLVAAVEKLQG"
     gene            2347..2919
                     /locus_tag="VN034_07895"
     CDS             2347..2919
                     /locus_tag="VN034_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HWV60559.1"
                     /translation="MDSAESEISRDPPAVSYPPNAVHLVRTNQQMTMQLSQMADQKAS
                     ILMGATFVVFTLAIGQARSGGGALAMPLAILATFSFLSALLAVSAVLPRVGKGPPVVY
                     KDGKDHSNILFFGRFAQMDEDEFIAEVKKRLRSEEDLYETMLRDTYQNGVVLARRKYR
                     YLAYAYRLFVVGLTLTFVAFGVEMAGLWSR"
     gene            3122..4519
                     /locus_tag="VN034_07900"
     CDS             3122..4519
                     /locus_tag="VN034_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWV60560.1"
                     /translation="MLQQAAPLTAETPQSLRAEFRATLALAVPLAAANLLQMLVHAID
                     VIFVARLGDAALAASSLGVAIFGLLLWTGSGLVGASAPLIAAELGRRKHAVREVRRTV
                     RMALWLSFFVSILFMGICAAGGPIMIATGQPPETSARAAGFLLILMWGMFPMIAAAVL
                     RIFVSALGRPTIATAITFGALFVNALGNWVLVFGHLGMPALGLHGSAISSVTTSFLML
                     LAYVVVIQSDRRLRRYHLFGNWWRSEWTRFFDMLRIGTPISLTILAEAGLFTGAAFLM
                     GRIGETELAGHTIALQVAALAFQIPFGVAQAATIRVGLFYGARDHNGIAHAGQASLIL
                     GIGFMGLTALIMWLFPSLVLSIYVDVDAARNAALVGFAMQFLVVAAAFQLFDGAQAVA
                     AGVLRGLQDTRTPMIIAICGYWIAGYGTAIYLGFWTPLAGVGVWIGLAVGLVVVSAVL
                     LLRWRMRARLGLLPA"
BASE COUNT          719 a         1510 c         1496 g          917 t
ORIGIN      
        1 ccgatcgcga tgaccgcggc ggcggcgccg agcagcgaaa agagcaggat cgcctggttg
       61 agactggcgc ggtcggccgc ggcgagccgg ttgcgctcgc gcagcagcgc gcgttcgttg
      121 gcgatgattt cgctgagcag attgtcgatc cgcgccagcc cgtcgccctg accggcggcg
      181 taaaatttgg aaatggcggc gacggtctgg ttatagttgg cgctgagcgc cgcgtcgccg
      241 agctgttcgc cgcggctgtc catctcgcgc gccaactgct cgaccagcgc gatctgtttg
      301 ggattgtcgc gcacattgcg gctgagccgc gtcatgaact ggcgcgcgcg gccccattga
      361 tattcataga tgcgcccgtc ctctttctgc agcccgaccg cgaagcggcc gagcgccgcc
      421 tcggcccgcg cgatcgcggc gtcgaccgaa cgcgtctgcg agatgacctc catgctctgc
      481 gtctgccagc cgagcgagcg gtcgtaattg tcgttggcgc gtgcgagcag cagcacgagc
      541 gccccgacca caccggtcag gatcgcaccg aggacgatcg tcgaccagaa gcgaatacgc
      601 tcccgccggc cgtcattttc gctgtcgata tcccgctcga acacgcgcat ctcttaccgg
      661 acaagcggtg cgccgcaaag caccgaaggt cgcgcggctt caattcgccg cgaaaaggaa
      721 tccgctggtc ggcgtgagcg ctcggttcag ccgataattt gcgtgcgctt gcccgcgcgt
      781 tcgaagcggc cgaggatttc gccgacctgc tcgctcgaat gctcggcgca cagcgaacag
      841 cgcagcagcg tcatgcccgc gggggtcgcc ggcggacgcg cgaggttcac atagagcccc
      901 tcttcgagca gcgcttccca catcatcgcg cccctttcga ggtcgggcat gatcaccgcg
      961 atgatcgccg actgcggctc gtcggtgccg agcgtgaagc ccagatcctt gagcccctgg
     1021 tggagcatct tcgaattttc ccacaggtgc gcgcgcttgt tgctcccgag catcagcttg
     1081 cggatgctcg tcgcggcggt cgcgacgacg ctcggcggca gcgaggcggt gaacacgtaa
     1141 ggacggcaga cgagccgcat gatctcgaac ttcggatggt tcgacacaca gaagccgccg
     1201 acggtgccga cgctcttcga aaaggtgccg atgatgaagt cgacatcgtc gatcacgccc
     1261 tgcgcctcgg cgacgccgcg gccatgctcg ccgatgaagc ccatcgaatg cgcctcgtcg
     1321 accagcacca tcgcgccatt ttccttggcg atgcggacca tttccttgag cggcgcgacg
     1381 tcgccgagca tcgaatagac gccttcgagc acgacgagct tgccggcgtc ggggggcagg
     1441 cgcttcaggc gcttttcgag cgcttcgatg tcgttgtggc gaaaggcgac gatttcggcg
     1501 tcgcccatct tgcacccgtc atagattgac gcgtggctgt cgatgtcgag gatgacatag
     1561 tcgcccttgc ccgcgatcgt cgaaatgatg ccgaggttcg cctgatatcc ggtcgaaaac
     1621 accatggcat ggtccatggc gtaaaattcc ctgagcgcgt cctcgcactc cttgtgcccc
     1681 tgataggtgc cgttgagcac gcggctgccg gtcgtcccgc tgccgaactt gtcgagcgcg
     1741 tccttgcccg cggcgatgac gtcctcgtcg aaggtcatgc ccatataatt ataggtgccg
     1801 agcaggatcg tctcgcgccc gttgcacatc gcgacggtcg gcgagaccac cttttccatc
     1861 accaggctga aagggtcggt gacgccggtg gcgagcagcg cctcgcgctg ctggatcagc
     1921 gggtcgaatt tggagaagag gtcggtcatc gcgctgatca gccctgcagc ttctcgaccg
     1981 cggcgacgag ctggccgaca ttctcgattt cggcctgcat gttcatgctg atgatgatgt
     2041 cgaacgtgtc ctcgacctcg gcgacgaaat ccatcaccgt caggctgtcc cattcgagat
     2101 cgccggcaaa ggtcgtcgcg tcgctcagct cgacgccctt cttgttgaag ggggcgatca
     2161 ggccgtaaat ctggtccttg atggcggtgc tcatatactg tgtcctatag cggaataaag
     2221 ttgcgcgcgg gcatgccgcg aagcctcccg attggcaagc gaatgcggcg ggaacagggc
     2281 agcatcttgc cgctcgcggg acttcatgcc atccctgtgg cgggtcgcca gcaggggcag
     2341 cttggcatgg acagcgcaga atcagagata tcgcgggatc cgccggcggt ctcctatccg
     2401 ccgaacgcgg tgcatctcgt gcgcaccaac cagcagatga cgatgcaatt gtcgcagatg
     2461 gcagatcaaa aggcgtcgat cctgatgggc gcgactttcg tcgtcttcac cctcgcgatc
     2521 ggacaggcgc ggtcgggcgg cggcgcgctc gcgatgccgc tcgccatact cgcgaccttt
     2581 tcctttctct cggcgctgct cgcggtgtcg gccgtgctcc cgcgcgtcgg caaggggccg
     2641 ccggtcgttt acaaggacgg caaggatcac agcaacatcc tcttcttcgg ccgcttcgcc
     2701 cagatggacg aggatgaatt catcgccgag gtgaagaagc ggctgcgcag cgaggaggat
     2761 ctgtacgaaa cgatgctgcg cgacacctat cagaacggcg tcgtcctcgc gcggcgcaaa
     2821 tatcgctacc ttgcctatgc ctaccggctg ttcgtcgtcg ggctgacgct gactttcgtg
     2881 gcgttcgggg tcgagatggc ggggctttgg agccgatgat ccaaatgtag tcgtcacccc
     2941 ggacttgatc cggggtccat ttgcgcagcg tcggaggaat ggatcccgga tcaagtccgg
     3001 gatgacgata gcggacggcg ggggcgctgc tgttgccatt ctgccgcccc cgccgacaaa
     3061 tcatccgccg ccgtctcgcc gcacttgaac ggtaacattc gcgcgcctat ccgggccggc
     3121 tatgctgcag caagccgccc ctttgaccgc ggaaacaccg cagagtcttc gcgccgaatt
     3181 ccgcgcgacg ctggcgctcg ccgtgccgct ggcggcggcg aacctcttgc agatgctcgt
     3241 tcacgcgatc gacgtcattt tcgtggcgcg gctcggcgac gccgcgctcg cggcctcgtc
     3301 gctcggggtc gcgatcttcg gcctgctgct gtggaccgga tcgggccttg tcggcgcatc
     3361 ggcgccgctg atcgccgccg aactcgggcg ccgcaaacat gcggtgcgcg aggtgcggcg
     3421 gaccgtgcgc atggcgctgt ggctcagctt tttcgtctcg atcctcttca tgggaatctg
     3481 cgccgcgggc ggaccgatca tgatcgccac cgggcagccg cccgaaactt cggcgcgcgc
     3541 cgcgggcttc ctgctgatcc tgatgtgggg catgttcccg atgatcgcgg ccgcggtgct
     3601 gcgaatcttt gtctcggcgc tcgggcggcc gaccattgcc acggcgatca ccttcggtgc
     3661 gctgttcgtc aatgcgctcg gcaattgggt gctcgtgttc ggccatctcg gcatgcccgc
     3721 gctcggcctc cacggctcgg cgatatcgag cgtgacgacc agctttctta tgctgctcgc
     3781 ctatgtcgtc gtcatccaga gcgaccggcg gctgcggcgc tatcatctgt tcggcaactg
     3841 gtggcggtcc gaatggacgc gctttttcga catgctgcgc atcggcaccc cgatcagcct
     3901 gacgattctg gccgaggcgg ggctgttcac cggcgccgcc ttcctgatgg gccgcatcgg
     3961 cgaaaccgag ctcgccgggc acacgatcgc gctccaggtc gcggcgcttg cgttccagat
     4021 tcccttcggc gtcgcgcagg cggcgacgat ccgcgtcggc cttttctatg gcgcgcgcga
     4081 tcacaacggc atcgcgcacg cggggcaggc gtcgctcatc ctcggcatcg gcttcatggg
     4141 gctgaccgcg ctcatcatgt ggcttttccc ctcgctcgtg ctgtcgatct atgtcgacgt
     4201 cgatgcggcg cgcaatgcgg cgctcgtcgg cttcgcgatg cagttcctcg tcgtcgccgc
     4261 cgccttccag ctcttcgatg gcgcgcaggc ggtggccgcg ggcgtgctgc gcggattgca
     4321 ggatacgcgc accccgatga tcatcgcgat ctgcggttat tggatcgcgg gttatggcac
     4381 tgccatctat ctcggcttct ggacgccgct cgccggagtc ggggtgtgga tcggtctcgc
     4441 ggtcggcctc gtggtggtct cggcagtgct gctgctgagg tggcggatgc gcgcgcgcct
     4501 cggcctgctt ccggcctgac gcaaaaactt cgcgagaatc ttgtacccct gcgcttgacg
     4561 cctatctgac tcccacccat atggccgact gttagcactc gcgtcctgtg agtgctaaac
     4621 gacatcattt ccatctctgg ag
//
LOCUS       DATMSU010000290        13846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_5468, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000290 DATMSU010000000
VERSION     DATMSU010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5468"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VN034_07905"
     CDS             <1..226
                     /locus_tag="VN034_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60561.1"
                     /translation="DQATAVLTVETLTARRANPGTSRAEALQMGMKAVRSGKRADGSA
                     VAGWKAGWAHPAAWAAFTNIANRDDEGVNP"
     gene            350..988
                     /locus_tag="VN034_07910"
     CDS             350..988
                     /locus_tag="VN034_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HWV60562.1"
                     /translation="MIIRSYRRVVYSLAILALLSGGTGAAKAPEAPGARDIDPGLRGP
                     VAGIGIESQDIVGMADRMVRDLLTNPTLSNRKIPPRIIVDSRYFTNESMQRLNGNLIT
                     DRLRVSLNRASQGRMVFIARQNAAMVEEERALKREGMTDIGTTGLTRAQAGADYRLTG
                     NIASADARDRRTGTMQRFTQITFEMVDLESGEIVWSNGYDIARAATDDVIYR"
     gene            1035..1160
                     /locus_tag="VN034_07915"
     CDS             1035..1160
                     /locus_tag="VN034_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60563.1"
                     /translation="MKYLSMAALLAMSILGGCTAAQQSIDPDAQVPPPAHPAAVD"
     gene            1162..1644
                     /locus_tag="VN034_07920"
     CDS             1162..1644
                     /locus_tag="VN034_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60564.1"
                     /translation="MGKNILLAIGTTALALSMSVPAVPAENRVVKCKTGAARKANAPA
                     GPALVANIPRSMSPIDLNAVQFIDRDLTKRVVVEGLFARRTPTDTVEITARLVNCTNA
                     PLQVMARSSFMDEAQAPTEATSAWSKLFIPAHGTGTYRETSISRDSVHYYLIELSAAR
                     "
     gene            1641..2477
                     /locus_tag="VN034_07925"
     CDS             1641..2477
                     /locus_tag="VN034_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60565.1"
                     /translation="MRRVILALACAPAALVALPAFAQYRDGMPPVIEAPVAAPVIGTD
                     PLSPAAFHDAYSRAGRPRLVIFWNRELTDQLATQSDRIVQVERSMSAASAAGISADGR
                     RAGRTDRSDSVETLRVGTREVDGERPIAALTNSTDWAVMSGFNSRLQSAGVLLVDRAV
                     ALRALAAASTAEDRQDRQTIETKALADKAEIILTVMQTPQSGTPLGAAFLVEAKRVNS
                     GEILATVSDTGEPPPAKPGRFVAGANGFVREAPRAPTAEEIGRELADRLMARLATNWN
                     AQ"
     gene            2474..4291
                     /locus_tag="VN034_07930"
     CDS             2474..4291
                     /locus_tag="VN034_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60566.1"
                     /translation="MRLHTPYILAGLVLFSVAAPAKAQVDSLQLPVTRVQLGEVVSGT
                     LTNDSAILQRNDESRPATVLAFPPIFPGEMYYFAIKSTRGEIRARLLEEVSEPGKPMK
                     LREMVLMKKRFRAGEKFGTGAAQFSGTRPIRIELRSSGPERDAAFEVGLMLYQNWDKW
                     RLEQAAMSALFEQNPARAINTRADRPADTTPWSDKAPLPAIEASTAVPLPAGFLETKA
                     AALQPFYRALYEGGEHSAVLNFERLGLAALELDLRKDAEWAFDQALDRIEAIYANNPV
                     AKAARSRYTLEAVKEFKGEPYERAMAYYYRGLLFLMADDYDNARAAFRSAEYQDTVSE
                     AEEFAGDFGMMHFLAGWSARCAGQEATAADDFKAAAAIDPALGAPPAGHDFLVLGEIG
                     RPPVKTKRGRSKEALIFGRSEDQGLDERAEVRPMRATDGQHAGRNAPPVSIEFANAAD
                     LHLQATTRGGRPIDALLRGKAVAKENLQEAADIIGSSMEFGLLGLIPQLLSDKVKADT
                     DVRMWDTLPDRVVLATAPARHAEGMELRFTGRDGPIEATPTVRHAAGRCAMAWSRSRS
                     ASKVQAGVPGHNQKSIAARVKSPDGAARDRQFREWLGTL"
     gene            complement(4379..5353)
                     /locus_tag="VN034_07935"
     CDS             complement(4379..5353)
                     /locus_tag="VN034_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HWV60567.1"
                     /translation="MTRISTVWKKGRAPVTMAFLLAVTLAMPAATQEAGTPDMPAVAG
                     SLPPPPDREARAAVVRADYRYDDLLWENDRTAHRIYGHALEAAEPPSGSGIDSWGKNV
                     RWPFADRQLRSGDQHSYRGEGLDFYNVGSTRGAGGLGIWHDNKLWTSRNYVGHRILSA
                     SGQAADFTVDYAPWPVDVNRKVWETRRFTLPLGTHFTRMVSTISSDSDEPLLVGIGIG
                     KRTTGPGAGELTVDRAKGLLSWWGPADGDHGRMAIAIRVDPAMIAEIRADADNQLVLL
                     RVAPGKPFVYFSGSAWDKGQGGFRTRQAWDAYAAGEKLDFKVPKGRIP"
     gene            5563..6336
                     /locus_tag="VN034_07940"
     CDS             5563..6336
                     /locus_tag="VN034_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWV60568.1"
                     /translation="MRQSGSDAVHDDRLALRKRRPSLVDAACEHIRTSIMSGVHRPGA
                     QLPTEAEFVDVLGVSRTVIREAIARLAAAGLVEARQGKGLFVSETARYQAFQVTRDEV
                     ENLSDVIQLLELRLCVETEMAALAAERRTEVDVMNMRQQIRILGEAAVGIEDSVKADV
                     EFHNAIARASKNTYYSKLIDFLGVRLVPPRSLYLRQGQAYIGDSYKAVISAEHEAILD
                     AIIHRDPDAARIAARTHMSGSLKRHRELHDFMSSSSPTG"
     gene            6503..9205
                     /locus_tag="VN034_07945"
     CDS             6503..9205
                     /locus_tag="VN034_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60569.1"
                     /translation="MAIAQTAPAADPVAGEGQDTSGDIVVTGIRQSLSRAAEIKREST
                     AVVDSIVAEDIGKLPDLTTASALQRVPGVQVVVGGNNEIVGARIRGLDDIVTTLNGRE
                     IFTGVGRGFSFQDLPAEALAGVDVYKSSSAERLEGGVAGGVNLRLRRALDFKDLTIAG
                     SARATYLQEAGSKNTINPALSLLVANRWSAGEGEIAAMVGISYRRNEYARPVAWNDWT
                     RATSAGPAGSPQNLHAPTGFAAAIEFGHYERPQANFSLEWAPDSDTTIYLEGLFAGYR
                     GDVFQARPTFRAYTGTSLEATAGDTCEDFAVGPDGYYSGNVRSPDNPTGTGTIRELCN
                     ATGYTARNIEYYTATVANKSSTDIYVIATGFNKEIGALTWNTDISYESSTNRNDSFRV
                     DIGKRIDELVLVRDVDHEVRATMPGNPMNDPEGLAFANGMSENINRSRGTLWAVMSDA
                     KYDFDGVLDYIQGGVRVAKRKAGFEQYLGGPPAPGGSFVTPLDTAGLPSDILSQAPGV
                     PQMNDGASFLQLDPEYLVQESIKRQLRTLYGISPDTPGFDPTRDYDAQEKSYAGFLQA
                     GYDIALTDTISIDGMVGARLTRTDRNIAGSGRVTDPVTGTSRVVLVRRSTSDTDLLPN
                     ASARIQFGGGLQSRFNYSKTLSRPSFGQLNPGLSYVVSYVNTIQNSGSGGNPDLRPQK
                     ADSFDASLEYYFGRSNYVSAVAFYRDITDRIINGTEVRTIDGLQYVISTPRNLGSAKI
                     KGVEVGGQLFLDFLPEGLDGAGIMGNFTVIDSEVTTPGDRLQGLPLLGVSKYNYNIGL
                     IYEKYGISARMIYTYRSQYFDGDITNGLSLRPVSIPVGLNGIRAAGRLDFGLNYDVAP
                     GITVSLAGTNITGQKTRNFESREDFVREVRNDDTTYSLGVRFNF"
     gene            9242..11398
                     /locus_tag="VN034_07950"
     CDS             9242..11398
                     /locus_tag="VN034_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60570.1"
                     /translation="MNRKRMFAALGLATALTAGSFALAGAAHAQTAATVLNQPGKALP
                     GGVRATRTQDSVTIDWPSAQDERAQLILSLDPGKPLIEAVSIAGKPVLGGVDPAGVVT
                     VGTRDLKQGWTIFFDNPRKRPFESFPLTLARSDISVRAEGGNTKVIVGGAAAGPFSGT
                     YEFTIYPGSRLVRAAMVMSTKREATAYTFDAGLGVASSAALPWRSIAFTGLSDEIVRK
                     EGDASREPAVSPKVRARMIVAEGAAGGSLGIVPPPHQFYYPLDYANNDNSAWYGNDYR
                     NLDGRTSFGVRQTLEGDRRYVPWVNAPPGTMQDMGVFLLPDSGDAASVTKAALAYTRG
                     DRFKAIDGHRTFTSHYHVEHTEAFLNTQRFQQSTGVPDGLESPEFVKRFKDMNVEIVH
                     LAELHLGREALDRAGDRLTLLKTMHAETARLSDDKLLLLPGEEPNVHLGGHWISFFPK
                     PVYWTLDRKEGQPFVENDPKLGKIYHVGSQEDVLKLMQDEKGLMWTAHPRIKGSTGFP
                     DKHKDTAFFKSDRFLGGAWKNMPVDYSQPRLGWRVLDTLDDMNNWASKPSERKFTPGE
                     VDVFQLSDESELYAHMNINYLRLDGPLPRYSDGWQSVLDALRGGRFFTTTGEVLIPEF
                     TIDGARSGEEAAFGRNATVRAKIEWTFPPAFAEIVSGDGSKVYRERIDLTGSAPFGAR
                     TIEKRIDLSGRKWARIEVWDVATNGAFTPPVFRKGK"
     gene            11513..12355
                     /gene="kduI"
                     /locus_tag="VN034_07955"
     CDS             11513..12355
                     /gene="kduI"
                     /locus_tag="VN034_07955"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HWV60571.1"
                     /translation="MFDRTYYATHPDMMARVSNDELRDRYLIRNLFRPNQCVLNYTHA
                     DRLVIGGVLVESGSVRLPDQSEPASAAGHPFLERRELGIVNVGRAEGAVTVDGEMFAL
                     GAKDSLYVTMGAKDVRFSGDGARFYLASSPAHRAFDTRKLPLADAKMLERGSLETAND
                     RTIHQLVIPGVCDSAQLAMGLTILKPGSVWNTMPPHVHERRSEIYFYFDLDDPEQGRV
                     MHFMGQGDATRHIVMTDGEAVISPPWSIHMGVGTSAYAFIWVMAGENQDYDDMNILDI
                     CQLR"
     gene            12758..13105
                     /locus_tag="VN034_07960"
     CDS             12758..13105
                     /locus_tag="VN034_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60572.1"
                     /translation="MHDNRESGISARERHQTLDLIGLRAHATAVGLIQLCEELLNAGV
                     LNMDGLDRIKDAICSELTVSKRARVSQQLNLDQSLRRRIDAVFPAAPGQPARDTIGSL
                     EDFEEALGGYHGA"
     gene            complement(13470..13685)
                     /locus_tag="VN034_07965"
     CDS             complement(13470..13685)
                     /locus_tag="VN034_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60573.1"
                     /translation="MRERRRHERFVEVLEKLRGSARPMISLPLLMSGLADDDDADLFV
                     PGASEKSKMLRVAGMRMTSSFDTKRFW"
BASE COUNT         2623 a         4512 c         4385 g         2326 t
ORIGIN      
        1 ggatcaggcg accgcggtgc tcacagtcga aacgctgacc gcgcgtcgcg cgaaccccgg
       61 cacctcgcgc gccgaggcgc tgcagatggg gatgaaagcg gtgcgcagcg gcaagcgcgc
      121 ggacggctcg gcggtcgcgg gctggaaagc cggttgggcg catcccgccg cctgggccgc
      181 cttcaccaac atagccaatc gcgatgacga gggcgtgaac ccataatagc gcgatcgacg
      241 tgttgagaag gcgagcaatg ccaagcgatt tttttcgcca taccccgttg gggggatgaa
      301 ataatctgac ctccgactag gacggcgcca tcgggcgaaa gggggtttcg tgatcatccg
      361 ttcttatcgg cgcgtcgtat attcgctggc gattctcgcg ctgttgagcg gcggcacggg
      421 cgccgcgaaa gcgcctgagg cgccgggcgc gcgggacatc gatcccggct tgcgaggccc
      481 cgttgccggt atcggcatcg aatcgcagga catcgtcggg atggccgacc gcatggtccg
      541 ggacctgctg acgaacccga cgctttccaa ccgcaagatc ccgccacgta tcatcgtcga
      601 tagccgctat ttcacgaatg aatcgatgca gcggctgaac gggaatctga tcaccgatcg
      661 gctgcgtgtg tcgctgaatc gtgcaagcca ggggcgcatg gttttcatcg cgcggcaaaa
      721 tgccgcgatg gtcgaagagg agcgcgcttt gaaacgcgag ggcatgaccg acatcggcac
      781 caccggattg acccgggcgc aggcgggagc cgattatcgc ctgacgggca atatagcgtc
      841 cgccgacgcg cgcgacaggc gtaccggaac catgcaacgc ttcacgcaaa taacgttcga
      901 aatggtcgat ctcgaatccg gtgagatcgt ctggtccaac ggctacgaca tcgctcgcgc
      961 cgcgaccgac gacgtcatct atcgttagga cgggggcccg cggccacgag cgtatcggga
     1021 aatagggagg gcgtatgaaa tatttatcga tggcggccct gctcgccatg tcgatcctcg
     1081 gcgggtgtac ggccgcacag cagtcgatcg atcccgatgc ccaggtgccg ccgccggccc
     1141 atcccgcagc ggtcgactga aatgggcaaa aacattctgc tcgcgatcgg tacgacggcg
     1201 ctggcgctct cgatgtccgt tcccgccgtt ccggcggaaa atcgcgtggt caagtgcaag
     1261 accggtgccg cgcgaaaagc gaacgctccc gcaggcccgg cgctcgtcgc caatatcccc
     1321 cgttcgatgt cgccgatcga tctgaacgcg gtgcaattca tcgaccgcga tctcaccaag
     1381 cgcgtcgtgg tcgagggcct gttcgcgcgc cgtacaccca ccgacacggt cgagataacg
     1441 gcgcggctgg tgaattgcac gaacgcgccg cttcaggtca tggcgcggtc ttcgttcatg
     1501 gatgaggcgc aggcaccgac cgaagcgacg tcggcgtggt cgaagctctt cattccagcg
     1561 catggcaccg gcacctatcg cgagacatcg atcagccgcg attccgtgca ttattatctg
     1621 atcgaattgt ccgcggcgcg atgaggcgcg ttattctagc cctcgcctgc gcgcccgccg
     1681 cgctcgtcgc gctgccggcc tttgcccagt accgcgacgg catgccgccg gtgatcgagg
     1741 ctcccgtcgc ggcgcccgtg atcggaaccg acccattgtc gccggcagcg tttcatgatg
     1801 cctattcgcg ggcgggcagg ccgcggctcg tgatattctg gaaccgcgaa ctcaccgacc
     1861 agctcgccac gcaaagcgac cggatcgtgc aagtcgagcg cagcatgtcg gcagcgagtg
     1921 cggcggggat cagcgccgat ggccggcgcg cgggacggac cgatcggtcg gatagtgtgg
     1981 aaacgcttcg cgtcgggacg cgcgaggtcg acggcgagcg gcctatcgcg gcgctgacaa
     2041 acagcaccga ctgggccgtg atgtcgggct tcaactcgcg gttgcagagc gccggcgttc
     2101 tcctcgtcga tcgcgccgtc gccctgcgcg cgctcgcagc cgcctcgacg gccgaagacc
     2161 ggcaggatcg ccaaacgatc gagacgaagg cgctcgccga caaggccgag atcattctga
     2221 cagtgatgca gacgccgcaa tccgggacgc cgctgggcgc ggccttcctc gtcgaggcaa
     2281 agcgcgtgaa cagcggtgaa attctcgcca ccgtctcgga cacgggcgag ccaccacctg
     2341 ccaaacccgg ccgcttcgtc gccggcgcga acgggttcgt gagagaagcg ccgcgcgcgc
     2401 cgaccgccga agaaattggc cgggaactgg ccgatcgact gatggcccgc ctggcgacga
     2461 actggaacgc ccaatgagac ttcatacccc gtatattctc gccgggctgg tcctcttctc
     2521 cgttgccgcg ccggcaaaag cgcaagtcga tagcctgcag cttccggtga cgcgcgtgca
     2581 actcggcgaa gtggtcagcg gcacgctgac aaatgacagt gcgatactgc aaagaaacga
     2641 tgagagtaga ccggcaacgg ttttggcgtt tcccccgatt tttcccgggg aaatgtatta
     2701 ttttgcgatc aagtcgacgc ggggtgaaat ccgtgcccgt ctccttgaag aagtgagcga
     2761 acccggcaag ccgatgaagc tgcgcgaaat ggttctcatg aaaaagcgat ttcgtgccgg
     2821 ggaaaagttc gggacgggcg cggctcagtt ctcggggacg agaccgatcc ggattgagct
     2881 gcgatcgtcg ggaccggaac gcgacgccgc tttcgaggtt ggcctcatgc tgtaccaaaa
     2941 ttgggacaag tggcgcctgg agcaggctgc aatgagcgcg ctgttcgagc aaaatcccgc
     3001 acgcgcgatc aatacgcgcg cagatcggcc ggcagacacg acaccctgga gcgacaaggc
     3061 gcctttgccc gcgatcgaag cgtcgaccgc cgtcccgctg cccgccggtt tcctcgagac
     3121 aaaggccgcg gcgctgcaac ccttttaccg cgccctttat gagggcgggg agcacagcgc
     3181 cgttctcaat ttcgagcggc tcggcctggc ggcgctcgaa ctcgatcttc gaaaggatgc
     3241 cgaatgggcc tttgatcagg cgctcgaccg gatcgaggcg atctatgcga acaacccggt
     3301 cgccaaggcc gcgcggtccc gttacacgct ggaagccgtc aaagagttca agggcgagcc
     3361 ttatgagcgc gccatggcct attattaccg gggcttgttg ttcctgatgg ccgacgacta
     3421 cgacaacgca cgcgctgcct tccggtcggc cgaatatcag gacaccgtat cggaagccga
     3481 agagttcgcc ggtgatttcg gcatgatgca ttttctggcg ggctggtccg cgcggtgcgc
     3541 ggggcaggaa gcgaccgcgg ccgacgattt caaggcggcc gccgcgattg atccggcgct
     3601 tggcgcgccg cccgccgggc atgattttct ggttctcggc gagatcgggc gaccgccggt
     3661 caagaccaaa cgcggcagga gcaaggaggc gcttatcttc ggccgatcgg aggaccaagg
     3721 gctcgacgaa cgagcggagg tccggccgat gcgagccacg gacggtcagc atgccggacg
     3781 aaatgcgccg ccagtctcca tcgagttcgc caacgcggcc gacctccatc ttcaagccac
     3841 cacgcgcggc ggccggccga tcgacgcgct cttgcgcggc aaggccgtcg ccaaggagaa
     3901 tctccaggaa gcggccgaca taatcggcag ttcgatggaa ttcggcctgc tcgggctgat
     3961 cccccagttg ctcagcgaca aggtcaaggc ggacaccgac gttcgcatgt gggacacctt
     4021 gcccgaccgt gtcgtcctgg ccaccgcacc agctcggcac gccgagggga tggagctgcg
     4081 gttcaccggt cgcgacggcc cgatagaggc gacgccgacg gtgcggcacg cggccggacg
     4141 gtgcgcgatg gcctggagcc gttcgcgatc ggcttcgaag gtccaggccg gcgttccggg
     4201 gcataaccag aaaagcatcg cggcgcgggt caagtcgccc gacggcgcag cgcgcgaccg
     4261 acaattcaga gagtggctcg ggacattatg aggtcgagac agtcaggacg ggggcttcat
     4321 tcgagcgtgc atctgcgccg ctcacctgaa ccatcgcgcg agttcacgca cgatgcttct
     4381 atggaatccg tcctttcggg accttgaagt ccagcttttc gcccgccgca taggcgtccc
     4441 atgcctggcg cgtgcgaaag ccgccctgcc ccttgtccca agccgaaccc gaaaaataga
     4501 cgaacggctt tccgggcgcg acgcgcagca ggacgagctg attgtccgcg tcggcgcgta
     4561 tctcggcgat catcgcggga tcgacccgga tcgcgatcgc catccggcca tgatccccgt
     4621 ccgcgggtcc ccaccacgaa agaagtccct tcgcacggtc gaccgtcagt tcgccggcgc
     4681 ccggccccgt cgtccgcttg ccgatgccga tcccgacgag gagcggctcg tcgctgtcgg
     4741 acgagatggt cgagaccatg cgggtgaaat gcgtaccgag cgggagcgtg aagcggcgcg
     4801 tctcccagac cttgcgattc acgtcgacgg gccatggcgc gtaatcgacg gtgaaatcgg
     4861 ccgcctgccc acttgcggac agaatgcggt ggccgacata atttcgcgac gtccacagct
     4921 tgttgtcgtg ccagatgccg agcccgccgg caccccgcgt cgaaccgaca ttgtagaagt
     4981 cgaggccttc gccgcgatag ctgtgctggt cgccgctgcg cagctggcga tcggcgaagg
     5041 gccagcgcac attcttgccc cacgaatcga ttcccgagcc cgagggcggt tccgccgctt
     5101 cgagcgcatg gccgtagatg cggtgcgcgg tgcgatcatt ctcccacaat agatcgtcgt
     5161 aacgatagtc ggcgcgcacc acggcagccc gagcttcccg gtcgggtggc ggcgggagcg
     5221 accccgcaac cgccggcatg tccggcgtcc cggcctcttg tgtcgccgcg ggcatcgcga
     5281 gggtcaccgc aagcaagaat gccattgtca ccggggcgcg cccctttttc cagacggtcg
     5341 aaatccgcgt catatctctc tccctggcag cgatccgatc ggtcgacgaa cccgccttat
     5401 gtcacgctgt gtgacgatgc ggcacccttg cggctatggc tcgcgggcct gatggatcaa
     5461 attttaaaat gaaatgaaat tatttaagtt gtcatatgac ttatagtcgc tacactcaag
     5521 cgggtatggt gcattatgtg ccggcaacgg gaggatgggc acgtgaggca gagcggcagc
     5581 gacgctgtgc atgacgacag gctggcgctt cgcaagcggc ggccgtcgct cgtcgatgcc
     5641 gcgtgcgagc atatccgcac cagcatcatg tcgggcgtgc atcggcccgg cgcccagctc
     5701 cctaccgagg ccgaattcgt cgatgtgctc ggcgtgagcc ggacggtgat tcgcgaagcg
     5761 atcgcccgtc tcgcggcggc gggactggtc gaggcccgcc agggaaaagg gctcttcgtc
     5821 agcgagaccg cacgctacca ggctttccag gtcacgcgcg acgaggtcga gaatctcagc
     5881 gatgtgatcc agctgctcga actgcggctg tgcgtcgaaa ccgagatggc ggcattggcg
     5941 gccgaacggc gtaccgaggt cgatgtgatg aacatgcgcc agcagatcag gatccttggc
     6001 gaggccgcgg tcggaattga ggattcggtc aaggccgatg tcgaattcca caacgccatc
     6061 gcccgggcga gcaagaacac ctattattcc aagctgatag atttcctcgg cgtccgcctc
     6121 gtgccccccc gctcgctcta tctgcgacag ggccaggcct atatcggcga cagctacaag
     6181 gcggtgatca gcgcggagca tgaagcgatc ctcgacgcca tcatccaccg cgaccccgac
     6241 gccgcgcgga tagcggcgcg aacgcatatg agcggcagcc tgaaacggca ccgcgaactc
     6301 cacgacttca tgtcgtcctc cagcccgacg ggctgaacca gaccaaaaca cagacctgca
     6361 gggaaactca ggcaccgaat tacgtccggc agaataataa gatcaatctg tcaaacttgt
     6421 atgatgtctt atagggagag gtccattgaa ggtcagtctt cgcgcctccg tggcgctgat
     6481 cgcactcgca agtggtgcgc ccatggcaat cgcgcaaacc gcgccagccg ccgatccggt
     6541 cgcgggtgaa gggcaggaca cgtcgggcga catcgtcgtc accggcatcc gccagagcct
     6601 gtcccgcgcc gcggagatca agcgcgagtc gaccgccgtg gtcgattcga tcgtcgccga
     6661 ggatatcggc aagcttcccg atcttaccac ggcgtcggcg ctccagcgcg tgcccggcgt
     6721 gcaggtggtg gtcggcggca acaacgagat cgtcggggcg cgtatccgcg gccttgacga
     6781 cattgtcacc acgctcaacg ggcgcgagat tttcaccggt gtcggtcgcg gcttctcgtt
     6841 ccaggatctc cccgccgaag cgctggcggg ggtcgacgtt tacaagtcca gttcggccga
     6901 acggctcgaa ggcggggtgg cgggcggcgt gaatctacgc ctgcgccgtg cgctcgactt
     6961 caaggacctg acgatcgcgg gcagcgcacg cgcgacctat ctgcaggaag cgggttcgaa
     7021 gaacacgatc aatccggcgc tgagcctgct cgtcgccaac cgctggtccg cgggtgaggg
     7081 cgagatagcg gcgatggtcg gtatttccta tcggcgcaat gaatatgccc ggccggttgc
     7141 gtggaacgac tggacccgcg cgaccagcgc ggggcccgcc ggatcgccgc agaatttgca
     7201 cgcgcccacc ggcttcgccg ccgcgatcga gttcggccat tacgagcgtc cgcaggcgaa
     7261 tttctcgctc gaatgggcac cggacagcga cacgacaatc tatctcgagg gtctgttcgc
     7321 ggggtatcgc ggcgacgtct tccaggcgcg cccgaccttc cgcgcctaca ccggcacgtc
     7381 gctcgaggcg accgcgggcg acacctgcga ggatttcgcg gtcggccccg acggctatta
     7441 ttcggggaat gtgcgcagcc cggacaatcc gacgggaacg ggtacgatcc gcgaactctg
     7501 caacgcgacc ggctataccg cgcgcaatat cgaatattac accgcgaccg tcgcgaacaa
     7561 gtcgagcacc gacatctatg tgatcgccac cggcttcaac aaggagatcg gcgcgctcac
     7621 ctggaatacc gacatttcct atgaatcctc gaccaaccgg aatgacagtt tccgcgtcga
     7681 cataggcaag cgcatcgacg aactggtcct agtccgcgac gtcgatcacg aggttcgggc
     7741 gacgatgccc ggcaatccga tgaacgaccc cgaaggcctg gcgttcgcca acggcatgag
     7801 cgaaaacatc aaccgcagcc ggggcacttt gtgggcggtc atgagcgacg cgaaatatga
     7861 tttcgacggg gtcctcgact atattcaggg cggcgttcgc gtcgcgaaac gcaaggcggg
     7921 attcgagcag tatctcggcg gcccgccggc gcctggcggc tcgttcgtga cgccgctcga
     7981 caccgcgggt ctccccagcg atattctttc gcaggctccc ggcgttccgc agatgaacga
     8041 tggcgcatcc ttcctgcagc tcgaccccga atatctggtg caggaatcga tcaagcggca
     8101 gctcaggacg ctgtacggca tctctcccga caccccgggc ttcgatccga cgcgcgacta
     8161 tgacgcgcag gaaaaaagct atgccggctt cctgcaggcg ggttacgaca tcgcgctgac
     8221 cgatacgatt tcgatcgacg gcatggtcgg cgcgcgcctg acccgcaccg atcgcaacat
     8281 cgcgggttcg ggccgggtga ccgatccggt gacgggcaca tcgcgcgtcg tgctggtccg
     8341 gcgctcgacc agcgacaccg acctcttgcc caacgccagc gcgcgcatcc agttcggcgg
     8401 cggcctccag tcgcgcttca attattcgaa gacactgtca cggcccagct tcggccagct
     8461 caaccccggc ctcagctatg tcgtatccta cgtcaacacg atccagaatt cgggatcggg
     8521 cggcaacccc gacctccggc cgcaaaaggc cgatagcttc gatgcgtcgc ttgaatatta
     8581 tttcggccgc agcaactatg tctcggcggt cgccttctat cgcgacatca cggaccggat
     8641 catcaacggc accgaggttc gcacgatcga cggcctgcaa tatgtgatct cgaccccccg
     8701 caatctgggc tccgccaaga tcaagggcgt cgaagtgggc ggccagctgt tcctcgactt
     8761 ccttccggaa ggactcgacg gtgcgggcat aatgggcaac ttcaccgtca tcgacagcga
     8821 agtgacgacc cccggcgacc ggcttcaggg cctgccgctg ctcggcgtct ccaaatataa
     8881 ttacaacatc ggcctgatct acgagaaata cggcatttca gcgcggatga tctacaccta
     8941 tcgctcgcaa tatttcgacg gcgacatcac caatggcctc tcgctgcggc cggtttcaat
     9001 cccggtgggg ctcaacggca ttcgcgccgc cggacggctc gacttcggcc tgaactacga
     9061 cgtggcgccg ggcatcacgg tcagcctcgc cggcacgaac atcacgggcc agaagacgcg
     9121 caacttcgaa tcgcgcgagg atttcgttcg cgaagtgcgg aacgacgaca ccacctactc
     9181 gctcggggtc cgcttcaact tctgagcctg aacgtgaatg ccggaataaa aggaggatga
     9241 cttgaacaga aaacggatgt tcgcggcgct tgggctggcg acggctttga ccgccggcag
     9301 cttcgccctc gcgggcgcgg cgcacgcgca gacggcggcc acggttctca accagcccgg
     9361 caaggcattg cccggcggcg tgcgcgccac acggacgcag gattcggtga cgatcgactg
     9421 gccatcggcg caggatgagc gcgcgcagct catcctgtcg ctcgacccgg gcaagccgct
     9481 gatcgaggcg gtctcgatcg ccggcaagcc ggtcctcggc ggcgtcgatc ctgccggtgt
     9541 cgtcaccgtc ggcacccgcg acctcaaaca gggctggacg atctttttcg acaacccccg
     9601 caagcgcccg ttcgaaagct tcccgctcac gctggcgcgc agcgacatca gcgtgcgcgc
     9661 ggagggcggc aataccaagg tgatcgtcgg cggcgcggct gccgggccgt tctcgggcac
     9721 ctatgaattc accatctacc ccggctcgcg gcttgtccgc gccgcgatgg tgatgtcgac
     9781 caagcgcgag gccaccgcct acaccttcga tgccggcctc ggggtcgcat cctcggccgc
     9841 cttgccgtgg cgctcgatcg ccttcaccgg cctgtccgac gagatcgtgc gcaaggaagg
     9901 cgatgcttcg cgggagcccg cggtctcgcc caaggtgcgc gcgcgaatga tcgtcgccga
     9961 gggcgccgcg ggcggttcgc tcggtatcgt cccgccgccg caccaattct actacccgct
    10021 cgactatgcc aacaacgaca attcggcctg gtacggcaat gactatcgca atctcgacgg
    10081 ccgcacgagc ttcggcgtgc gccagacgct cgagggtgat cggcgctatg tgccgtgggt
    10141 caacgccccg cccggcacga tgcaggacat gggcgtgttc ctgctgcccg acagcggcga
    10201 tgcggcgagt gtcaccaagg ccgcgctcgc ctatacccgc ggcgaccgct tcaaggcgat
    10261 cgacgggcac cgcaccttca ccagccatta tcatgtcgag cataccgaag cctttctgaa
    10321 cacgcagcgc ttccagcagt cgaccggcgt gcccgacggg ctggagtcgc ccgagttcgt
    10381 caagcgcttc aaggacatga acgtcgagat cgtccacctc gccgagcttc acctcgggcg
    10441 cgaggcgctc gatcgcgcgg gcgaccggct gaccttgctc aagacgatgc acgccgagac
    10501 cgcgcggctg tcggacgaca agctgctgct gctccccggc gaggagccca acgtccatct
    10561 cggcgggcac tggatcagct tcttccccaa gccggtctat tggacgctcg accgcaagga
    10621 agggcagccg ttcgtcgaaa atgatcccaa gctgggcaag atctatcatg tcggctcgca
    10681 ggaagatgtc ctgaagctga tgcaggacga gaagggcctg atgtggaccg cgcacccgcg
    10741 gatcaagggt tcgacgggct tccccgacaa gcacaaggac accgccttct tcaagagcga
    10801 ccgcttcctc ggcggtgcgt ggaagaatat gccggtggac tattcgcagc cgcgcctcgg
    10861 ttggcgcgtg ctcgacacgc tcgacgacat gaacaattgg gcgtcgaaac cgtccgagcg
    10921 caagttcacc cccggcgagg tcgatgtgtt ccagctctcc gacgagagcg agctctatgc
    10981 gcatatgaac atcaactacc tccggctcga cgggccgctg ccgcgctatt ccgatggctg
    11041 gcagagcgtg ctcgacgcgc tgcgcggggg ccgcttcttc accaccaccg gcgaagtgct
    11101 gatccccgaa ttcacgatcg acggcgcgag gagcggcgaa gaagccgcgt tcggccggaa
    11161 tgcgacggtg cgcgcgaaga tcgaatggac cttccccccc gccttcgccg agatcgtcag
    11221 cggcgacggc agcaaagtct atcgcgagcg catcgacctc accggcagcg cgcccttcgg
    11281 cgcccgcacg atcgaaaaac ggatcgacct cagcggccgc aaatgggcgc gcatcgaagt
    11341 gtgggacgtc gcgacgaacg gcgcgttcac gccgccggtc ttccggaaag ggaaatagcc
    11401 ctcccccctg cccgtgcagt cgtcagcgca tcccctctga cgactgcacg tggccctttt
    11461 gaatcggagc gggctcgccg gcgcgccggt ccggtccttc tttggagcaa ccatgttcga
    11521 ccgaacctat tacgccaccc atcccgacat gatggcgcgc gtgtccaatg acgaactgcg
    11581 cgatcgctat ctcatccgga atttgttccg ccccaaccag tgcgtgctca actatacgca
    11641 tgccgaccgg ctggtgatcg gcggcgtgct ggtcgagagc ggaagcgtca ggctacccga
    11701 ccaatcggag cccgcgtcgg ctgccggcca cccgttcctc gagcggcgcg aactcggcat
    11761 cgtcaatgtc ggccgcgccg agggcgccgt gaccgtcgac ggcgagatgt tcgcgctcgg
    11821 cgcgaaggac agcctgtatg tcacgatggg cgcgaaagac gtccgcttct ccggcgacgg
    11881 cgcccgcttc tatctcgcct cgtcccccgc gcaccgtgcc ttcgatacgc gcaaactgcc
    11941 gctcgccgac gccaagatgc tcgagcgcgg cagcctggag acggccaacg accggacgat
    12001 tcaccagctc gtcatccccg gcgtgtgcga cagcgcgcaa ctggcgatgg gcctcacgat
    12061 cctcaagccg ggaagcgtgt ggaacacgat gccgccgcac gtgcacgaac gccggagcga
    12121 gatctatttc tacttcgatc tcgacgatcc cgagcagggc cgggtgatgc acttcatggg
    12181 ccagggcgat gccacgcgtc atatcgtcat gacggacggc gaggcggtga tctccccgcc
    12241 ctggtcgatc cacatgggcg tagggaccag cgcctatgcc ttcatctggg tcatggcggg
    12301 tgagaaccag gactatgacg acatgaacat cctcgacatc tgccagctcc gctgattatc
    12361 gatatcgatt gccgatcgcc ggaaatggcg cacgactggc tccggccgcc acgtcggccg
    12421 catgaacggc ggcaatgagg cgcaaggacg actgccgcgc gctcgggagc ggcatcacca
    12481 tgctgccgcc tccgggccgg atcccacttc gcggtgatgg cgggtgtcat caaacgcgcg
    12541 cccatgtgcg ccgacccaag aatttgtaac gcaatgattt ttgcagcccg cccgcgagag
    12601 cgaaagtggc gtgccgatga caacacgaat ggaacgattt cttgatggtc tggaacggcg
    12661 gcgcacgccc gtcgttgaaa aggagcgacc ctaagaagcc acgcgccctc ggctgggccg
    12721 aggccggacc aatgggccca accgatggag aggatggatg catgacaacc gcgagtcggg
    12781 gataagcgcg cgcgaacgac atcagacgct cgacctgatc ggtcttcgcg cccatgcgac
    12841 cgctgtcggg ctgatccagc tctgcgagga actgctgaac gccggtgtgc tcaacatgga
    12901 cgggctcgac cgcatcaagg atgcgatctg ctcggaactg accgtttcga aacgcgcacg
    12961 cgtttcacag caactgaacc tcgatcagtc gctccgccgc cgcatcgatg cggtcttccc
    13021 ggcggcgccc ggccaacccg cccgcgacac gatcgggtcg ctggaggatt tcgaggaagc
    13081 cctcggcgga tatcacggtg cctgatgact ccagatatat cgaaggaaaa cagcgagcaa
    13141 ttgtcgccgc gtgaccgggc gaacctgaca ttggaagaac tcctggttcg accgcagcaa
    13201 gcgacggacc atcagacgtc gatcgctcga cacgcccccc agcggggcga tgctgcggag
    13261 cggctcttca tcgcccaagg atatgcggcc accagtctcg ccgatatcgt ccgcacctcc
    13321 cgcgggctcg ctcgccacgc tctactaact gttcggcaac aagcagggat tgcttcatgc
    13381 gctcgccatc cgatggcgcg acaaataggg taaagcggac tttcggacct cgcgccggcc
    13441 cggcgacgat gagcggaggg gggatccgat caccagaacc gcttggtgtc aaaagaactc
    13501 gtcatacgca ttccggctac gcgcagcatc ttcgatttct ctgaagcgcc cggtacgaac
    13561 aagtcggcgt cgtcatcatc cgccagcccg ctcattagaa gcggcagcga gatcattggg
    13621 cgcgccgagc ccctcagctt ttccaacact tcgacaaagc gttcgtgccg ccgccgttcg
    13681 cgcatccacg cgaacagcga ggcgaaggta agcgtgtcct gaaggccgcc ttgcaggaag
    13741 ctgctgcgcg gtgctgatag ccgctctttg cgaggagcgg tgatgaacga ggatatcgcg
    13801 gcgacgggag cgagtgctct tacgaccggt catatgagtg atcata
//
LOCUS       DATMSU010000291         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_146796,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000291 DATMSU010000000
VERSION     DATMSU010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146796"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..191)
                     /locus_tag="VN034_07970"
     CDS             complement(36..191)
                     /locus_tag="VN034_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60574.1"
                     /translation="MDEDRLLADMLALAERLAESDDALMAGQYALLRARVAALIELRA
                     FGKDLAA"
     gene            complement(202..1602)
                     /locus_tag="VN034_07975"
     CDS             complement(202..1602)
                     /locus_tag="VN034_07975"
                     /inference="COORDINATES: protein motif:HMM:NF025888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60575.1"
                     /translation="MAASLADPFAASPLLRRFAGATRAELEAYQLRKVQALCARLYAK
                     SDFYRTRMDEGGLKGGAVDSLDRFAKAFPTSAKADFLADQQVAPPFGTRLGVDRDEVV
                     HISMTSGTSGQGQEIYGRTQRDVHMLGYLHALPWYMGGLRQGDTAINCVPAGGMTTGG
                     WGPGEALRIMGATGFHVGGNTSTEAKIDLMLGLGGVHFIYASTNYLHTLTEALLARGI
                     TPREAFPAMHGIFIAAEGYPLEWAAKIEEHWGCRLQEGYGSTQLNGFGGSTGSAGVFR
                     EDGQRGLMRLFEWEHLIETVDPETGAPVQPGEQGEMVVTNLSVEGSPVVRFRTGDAAR
                     FVPWQECGGGAWNAIECGTIGRFDDMMKIRGNNVWPSMFDAAIFAHPEVGEYRGRVYT
                     RDSKTEVEVKIAVADHHANLCEDDRAALVQSVRAAIKQRSNMWVDVCEVPRAEFQGFA
                     YKARRWADERQDGYRL"
     gene            complement(1606..2025)
                     /locus_tag="VN034_07980"
     CDS             complement(1606..2025)
                     /locus_tag="VN034_07980"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HWV60576.1"
                     /translation="MQKTAYSRPLPVPDSESTPFWDGMREGKLMLQRCASSGEYLFPP
                     VTFCPGSLERPAWTEASGKGEVFSWIVVRHPVPRDLYADEVPYVVAIVALDEGCRMTG
                     NIVDCAPEDMRAGMKVEIVYNRVTPEITLPAFRPKGG"
     gene            complement(2029..3186)
                     /locus_tag="VN034_07985"
     CDS             complement(2029..3186)
                     /locus_tag="VN034_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWV60577.1"
                     /translation="MFKLSDKYAIAGIGNTDYSKFSGRTVRSLATEACLKAIDDAGLT
                     VADIDGIVSFNFNDSAPAMGVATEMGIENAGYAVDYAAGGNGANLITLAAIAAIEAGL
                     AKNVLCFRAMNGRSGFRLGGGRDMSAYGVTQYTAPLGWITYPQAMAMWARRHMIDYGT
                     TEEHWAEIATTFRDNAVINERAMQRTPMTKDDYYNSRFIVDPFRMYDICLESDGACAV
                     VITSAEQARDLKQKPVYIMGGAYGGGPSQGDDLFDAIRWPSHSHNCFKYLADDLWGSA
                     GIAPKDVDVAEIYDCFTYSVLMALEGLGFCKEGEGGPFVMGGRIARTGELPLNTHGGL
                     LSEAYIHGFNHVIEAVEQLRGTAGARQIDGAEIALTTAGAMTCGSAMILRN"
     gene            complement(3188..3985)
                     /locus_tag="VN034_07990"
     CDS             complement(3188..3985)
                     /locus_tag="VN034_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWV60578.1"
                     /translation="MTYQNLKLVYDGRIARVTLARPDKLNPLDWSAVKELKAVVAEIE
                     AAPGVDFVLLTGEGRSFSAGGDLDGYVRLYAHPDEFQAFLGDFYDMLTGIEQARAIWV
                     AAVNGVCVAGGIELLLACDMALAAESAKIGDGHLNFGQLPGAGGSQRLPRAVGLLRAK
                     HLMLTGELLDAHTSESWGLVTKVVPDAELIAAAEEWIAGMATKSAIGLAGAKRLANMT
                     LDTPYAEGLRQEIAFVHRYATTEPDATEGLLAFKEKRAPRFGARKED"
     gene            complement(3982..>5301)
                     /locus_tag="VN034_07995"
     CDS             complement(3982..>5301)
                     /locus_tag="VN034_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWV60579.1"
                     /translation="GMVAVGIGARAGVAEIAHLLKRTGAKLLIAHEQMRDRTLPELVE
                     AVRAQGAPLDGWLTVPDLIARRNWSAGDVADHPPLPAPDPDALFLINSTSGTTGMPKC
                     VMHTQNRWYYFHQLAARAGRFGDDEIFMGAVPMPFGFGLWTAHFSPTILGCPTVVLPK
                     FDAGTALDMIERERVTVLCCVSTQFIMLLNEQAERPRDLSSLKCMFTGGEAVPYRRAA
                     EFEEVTGAAVLQFYGSNETGALSNTTAADTRDRRLNTAGQIIPEMEVRLFDPATGEEV
                     EGSGQPGCRGPALCLGYWDDDAANARLFTPDGWMLMGDIVDIDADDYLRVVGRTSEFI
                     IRGGKNLSAPAIEAEVATHPAVALVAAVPAPDAVFGERVCIYVAPRPNQQVTLEAITS
                     HLQTRGVSREWFPEYLVLMDDLPRSSGGKVAKGELAADAKKRWAVPV"
BASE COUNT          872 a         1797 c         1712 g          920 t
ORIGIN      
        1 gacctggcca tcgagcgcga agcgcgccac gccggtcatg ctgccaggtc cttaccgaac
       61 gcgcgcagct cgatcagcgc cgcgacgcgc gcacgcagca gcgcatattg ccccgccatc
      121 agcgcgtcgt cgctttccgc cagccgctcg gcaagcgcca gcatgtcggc gagaagcctg
      181 tcttcgtcca tcgtcccgct cctacagccg gtatccgtcc tgccgctcat cggcccagcg
      241 gcgcgccttg taggcaaagc cctggaactc ggcgcgtggc acttcgcaca cgtcgaccca
      301 catattgctg cgctgcttga tcgcggcgcg caccgactgg accagcgccg cgcgatcgtc
      361 ttcgcacaga ttcgcatggt gatcggcgac cgcgatcttc acctcgacct cggtcttgct
      421 gtcgcgcgtg tagacgcggc ctctatattc gccgacctcg ggatgcgcga agatcgcggc
      481 gtcgaacatc gacggccaga cattgttgcc gcggatcttc atcatgtcgt cgaagcggcc
      541 gatcgtgccg cactcgatcg cgttccacgc gccgccgccg cattcctgcc agggcacgaa
      601 gcgcgccgcg tcgccggtgc gaaagcgcac caccgggctg ccctcgaccg acaggttggt
      661 gacgaccatt tcgccctgtt cgcccggctg taccggcgcg cccgtttcgg gatcgacggt
      721 ctcgatcaga tgctcccatt cgaacaggcg catcagcccg cgttgcccgt cttcgcggaa
      781 cacccccgcc gacccggtcg aaccgccaaa gccgttcaat tgcgtgctgc catagccttc
      841 ctgcaggcgg cagccccaat gttcctcgat cttcgccgcc cattcgagcg gatagccctc
      901 ggccgcgatg aagatgccgt gcatggcggg gaaggcttcg cgcggcgtga tgccgcgcgc
      961 aagcagcgcc tcggtcagtg tgtggaggta gttggtcgag gcatagatga aatgcacgcc
     1021 cccgaggccc agcatcaggt cgatcttcgc ttcggtcgag gtgttgcccc cgacgtgaaa
     1081 ccccgtcgcg cccatgatcc gcagcgcctc gcccggcccc cagccgccgg tcgtcatgcc
     1141 gcccgcgggg acgcagttga tcgccgtgtc gccctgacgc agcccgccca tgtaccatgg
     1201 caaggcatgg agatagccga gcatatggac gtcacgctgg gtgcggccgt aaatctcctg
     1261 cccctgcccc gatgtgccgc tcgtcatgct gatatgcacg acctcgtcgc gatcgacgcc
     1321 gagccgcgtg ccaaagggcg gcgcgacctg ctggtcggcg aggaagtcgg ccttggccga
     1381 ggtcgggaag gccttggcga agcggtcgag gctgtcgacc gcgccgccct tcaacccgcc
     1441 ctcgtccatc cgcgtgcggt agaaatcgct cttcgcatag agccgcgcgc acagcgcctg
     1501 caccttgcgc agttgatacg cctccagttc ggcgcgcgtc gcgccggcaa agcggcggag
     1561 cagcggcgag gcggcgaagg gatcggcgag agacgcagcc atcgctcagc ctcccttagg
     1621 tcggaacgcc ggcagcgtga tctccggcgt gacgcggtta tagacgatct cgaccttcat
     1681 cccggcgcgc atgtcctcgg gtgcgcagtc gacgatgttg ccggtcatcc ggcacccttc
     1741 gtcgagcgcg acgatcgcga ccacataggg cacctcgtcg gcgtaaaggt cgcgcggtac
     1801 cgggtggcgc acgacgatcc agctgaaaac ctcgcccttg ccgctcgcct cggtccacgc
     1861 gggccgttcg agcgagccgg ggcagaaggt caccggcggg aagagatatt cgccgctcga
     1921 tgcgcagcgc tggagcatca atttgccctc gcgcatcccg tcccagaagg gcgtgctttc
     1981 ggaatcggga acgggcagcg gccgcgaata ggcagttttt tgcatcggtc agttcctcag
     2041 gatcatggcg ctgccgcagg tcatcgcgcc ggcggtggtg agcgcgattt cggcgccgtc
     2101 gatctggcgc gcgccggcgg taccgcgcag ctgctcgacc gcctcgatga catgattgaa
     2161 gccatggata taggcttcgg acagcaagcc gccgtgggtg ttgagtggca gctcgccggt
     2221 gcgcgcaatg cggccgccca tcacgaatgg gcctccctcg ccttccttgc aaaagccgag
     2281 gccttcgagc gccatcagca cgctgtaagt gaaacagtcg tagatttcgg cgacgtcgac
     2341 atccttggga gcgatgcccg cgctgcccca cagatcgtcg gcgagatatt tgaagcaatt
     2401 gtgcgagtgg ctgggccagc ggatcgcgtc gaacaggtcg tcgccctggc tcggtccgcc
     2461 gccataggcg ccgcccatga tatagacggg cttctgcttg agatcgcgcg cttgctcggc
     2521 cgacgtgatc accaccgcgc aggcgccgtc gctctccaga cagatgtcgt acatgcggaa
     2581 tgggtcgacg atgaagcgcg agttgtaata atcgtccttg gtcatcgggg tgcgctgcat
     2641 cgcgcgttcg ttgatcaccg cattgtcgcg aaaggtcgtc gcgatctcgg cccaatgttc
     2701 ctcggtcgtg ccataatcga tcatgtggcg ccgcgcccac atcgccatcg cttgcggata
     2761 ggtgatccag ccgagcggcg cggtatattg cgtcacgcca taagccgaca tgtcgcgccc
     2821 gccgccgagc cggaagcccg agcgcccgtt catcgcgcgg aagcagagga cattcttcgc
     2881 cagccccgcc tcgatcgccg cgatcgccgc gagcgtgatc aggttcgcgc cattgccgcc
     2941 cgcggcatag tcgaccgcat agccggcatt ttcgatgccc atctcggtcg caacgcccat
     3001 tgccggcgcg ctgtcgttga agttgaagct gacgatgccg tcgatgtcgg cgacggtcag
     3061 cccggcatcg tcgatcgcct tcaggcacgc ctcggtcgcg aggctccgca ccgtgcggcc
     3121 cgaaaatttg ctgtaatcgg tgttgccgat cccggcgatc gcatatttgt cgctgagttt
     3181 gaacatgtca gtcttccttt cgcgcgccga aacgcggcgc gcgtttttcc ttgaaggcga
     3241 gaaggccttc ggtggcatcg ggttcggtgg tcgcgtagcg gtggacaaag gcgatttcct
     3301 gccgcagccc ctccgcatag ggcgtgtcga gcgtcatgtt cgcgagccgc ttggcacccg
     3361 cgagaccgat cgcgctcttc gtcgccatgc cggcaatcca ttcttcggcg gcggcgatca
     3421 actcggcatc ggggacgact ttggtgacga gcccccagct ttcggacgtg tgcgcatcga
     3481 gcagctcacc cgtcagcatc agatgcttgg cgcgcagcag cccaaccgcg cgcggcaacc
     3541 gctgcgatcc gccagccccc ggcaactgac cgaagttcaa atgcccgtcg ccgatcttcg
     3601 cgctctcggc ggcgagcgcc atgtcgcacg cgagcagcaa ttcgatcccg cccgcgacac
     3661 acacaccatt gaccgcggcg acccagatcg cgcgcgcctg ctcgatcccg gtgagcatgt
     3721 cgtaaaagtc gccgaggaac gcctgaaact cgtcggggtg cgcgtagagg cggacatagc
     3781 cgtcgagatc gccgcccgcc gagaagctgc gtccctcgcc ggtgagcagc acgaagtcga
     3841 caccgggcgc cgcctcgatc tcggcaacga cggctttcaa ctccttgacc gccgaccagt
     3901 cgagcggatt gagcttgtcg ggccgcgcca gcgtgacgcg ggcgatgcgg ccgtcataga
     3961 cgagtttgag gttctgatac gtcataccgg caccgcccag cgttttttcg catcggcggc
     4021 gagttcgccc ttggcgacct tgccacccga cgagcgcggc agatcgtcca tcaggacgag
     4081 atattcgggg aaccattcgc gcgacacgcc gcgcgtctgg agatggctgg tgatcgcctc
     4141 gagcgtcacc tgctggttcg ggcgcggggc gacatagatg cacacgcgtt cgccgaacac
     4201 cgcatcgggc gcgggcaccg cggcgacgag cgcgaccgct ggatgcgtcg caacctcggc
     4261 ctcgatcgcg ggcgccgaca gattcttgcc gccgcgaatg ataaattcgg acgtccggcc
     4321 gacgacgcgc agataatcgt cggcgtcgat gtcgacgata tcgcccatca gcatccagcc
     4381 gtcgggggtg aacagcctgg cgttcgccgc gtcatcgtcc caatagccga ggcacaaagc
     4441 ggggccgcgg caacccggct ggccgctgcc ttcgacctct tcgccggtgg cggggtcgaa
     4501 caggcgaacc tccatctcgg ggatgatctg ccccgcggtg ttgaggcgcc ggtcgcgcgt
     4561 atcggcggcg gtggtgttgc tgagcgcgcc agtctcgttc gatccgtaga attgcagcac
     4621 cgcggcgccg gtgacctcct cgaactcggc ggcgcggcga tagggtaccg cttcgccgcc
     4681 ggtgaacatg catttgagcg acgacaggtc gcgcggacgc tcggcctgtt cgttgagcag
     4741 catgatgaac tgggtcgaga cgcagcacag caccgtcacc cgctcgcgtt cgatcatgtc
     4801 gagcgcggtg ccggcatcga acttgggcag tacgacggtc gggcagccga ggatcgtcgg
     4861 cgagaaatgc gcggtccaca gcccgaaacc gaagggcatc ggcaccgcgc ccatgaagat
     4921 ttcatcgtcg ccgaagcgcc ccgccctggc cgcaagctgg tggaaataat accagcgatt
     4981 ctgcgtgtgc atcacgcatt tgggcattcc cgtcgtgccc gaggtcgagt tgatcaggaa
     5041 caaggcgtcg ggatcggggg ccggcaacgg cggatgatcg gcaacgtcgc cggcgctcca
     5101 gttgcgccgc gcgatcagat cggggaccgt aagccagccg tcgagcgggg cgccttgggc
     5161 gcggacggcc tcgaccagtt ccgggagcgt gcggtcgcgc atctgttcgt gggcgatcag
     5221 caacttcgcg ccggtgcgtt tcagcaaatg cgcgatctcg gcgaccccgg cgcgcgcgcc
     5281 gatgccgacc gcgaccatac c
//
LOCUS       DATMSU010000292         7238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_185058,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000292 DATMSU010000000
VERSION     DATMSU010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7238
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185058"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VN034_08000"
     CDS             complement(<1..627)
                     /locus_tag="VN034_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWV60580.1"
                     /translation="MTLALLGFATVLLFIILIMTKRMSAAAALIAIPIAGGLLAGAGP
                     QIGDMMLKGILDVAPIATMLAFAVLYFAVMMDSGLFDPLVNRILAMVGDDPVRIAIGT
                     MALSTLVSLDGDGTTTALIVITSMLPLYRAIGMNPLILATLLGSSNAIMNLLPWGGPS
                     ARAAAAVKVDLVHDVFLPLVPAMLIALAALAALAWWLGTRERMRLGWEP"
     gene            complement(676..1455)
                     /locus_tag="VN034_08005"
     CDS             complement(676..1455)
                     /locus_tag="VN034_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWV60581.1"
                     /translation="MATTSQGAPGARGDIDEAEWAVRVDLAAAYRLVALHGWDDLIFT
                     HLSARVPGPDHHFLINPYTHMFEEITASSLVKIDIHGKQVDGAPAPVNAAGFTIHSAI
                     HMARADAAAVLHLHTPAGQAVSAMRCGLLPHTQTAMIVHHDLAYHDYEGIATNLAERE
                     RIVADLGDRQAMLLRNHGTLAVGRSVADAFVRIYFLERACEAQVMMLGAGRDGLHDPE
                     GKVADVVRDQVRAPGMQAASELLAWPALLRKLDRIDPGFRD"
     gene            complement(1489..1800)
                     /locus_tag="VN034_08010"
     CDS             complement(1489..1800)
                     /locus_tag="VN034_08010"
                     /inference="COORDINATES: protein motif:HMM:NF025688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4387 domain-containing protein"
                     /protein_id="HWV60582.1"
                     /translation="MVKLRDVCHEIRSKNAGPFWVTFDIFFDGPDNFAKYHQDPALSA
                     DSFARLYGAEAAMVKRIPVDDLSMIKISYPRTTPQGGMVERDMHSGQQYVPLLDVELA
                     G"
     gene            complement(1793..3232)
                     /locus_tag="VN034_08015"
     CDS             complement(1793..3232)
                     /locus_tag="VN034_08015"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HWV60583.1"
                     /translation="MTEQPQAAGAFAGPIKVLVPCGSLGAGVRESEIAYGIAEGAHVI
                     ASDAGSTDSGAAYLALGKSKNSRGAVKRDLEILMKAQAEHGIPIIIGTSGQAGGDLNL
                     EWTREIVEEVAAELAMAPRIALIHCEQDKATVKRLNAEGRIHPLPPLGPLADEIVDQC
                     EHIVAALGVEHFIAALDGGADIILGGRSTDTAVLACYPIWKGAPWGPAWHAGKTGECG
                     VQCSVNPTLGSGVMLTVKSDGFEVAPLSDDNRCSVHSVSAHMLYENTDPFRLAEPGGV
                     LDVTRARYTQKDDRTVSVSGSEWEPGPYSMKLEGASAGQYQTMMLVGIRDPEVLNDIE
                     NFHDKLLAALYDRTRKSFPAEVLGEFHISLRLYGWNGIDGKPVRPGTPPPPEIGMIFV
                     ATADTQELANAIAHACNPYFFHFPNVMNKEIPSYGFMLSPADVPCGQVYEFRLNHIVA
                     LDDPFELVRIEYVDLASRSVEARKEVAHG"
     gene            complement(3276..5441)
                     /locus_tag="VN034_08020"
     CDS             complement(3276..5441)
                     /locus_tag="VN034_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60584.1"
                     /translation="MRGAILFCGAAVLALSNAQAARAQDGSEAPGAARADDDAIIVTA
                     QRRAQSLQDVPVTVTVFGAEEIQQARIQQVSDVVTRTPGLSFDAFPASQPRLAVRGIG
                     SSDKGAAGDPSAAVFLDEIYLGRPAAVAFDAFDVERIEVLKGPQGTLFGRNVVGGAIN
                     VVTKRPELGGFDAAAEFTYGNYDRLDGAGFVNLPFGGGGGAIRISGAYRSHGGYVYNP
                     VIDRRVEDQDTLSGRFQFLAEPTDNLRVHFTLDGTRDRATGPAKYIFDVDPDDPLSAA
                     YTVDRDPKRTYGSFAGYQDRDTIGVRGEIAWDMSFATLSFLGSYRDLEYGSRYDFDGS
                     NPTRSLVDISGGEDERSELSSQEVRLSSLPGSGIDWVVGFYHYRQETVRSDIFNLDSE
                     FVAPIPLTDIYDQDARLDSIALFGDATIPLGDRFSIIGGVRYSRDDKTYRVTNTRGMA
                     PLRGEEFFDVTTQKSWDDVTYRGGLNFKPNDDHLLYAMVSRGFKSGGFQDTPSSAIDA
                     ATAFDPETAVQYEIGQKSRFFDGRLIWNNTLYWLDYRDLQTRQSQPDGSIITNNAGKA
                     TIKGYEMQLTARLFDGLNLSAAYAYTDARFDEFDDEGIDRSGNRISRTPKHKLTLSPS
                     YTVALGGRTELTVAADYQYESLIYDDNSNEGPEVRPATHFVDARVVLAGFGDHWTLSL
                     WGKNLTNERTRTHQTLFLGANFGTYNPPRTYGATLGWRY"
     gene            complement(5584..6336)
                     /locus_tag="VN034_08025"
     CDS             complement(5584..6336)
                     /locus_tag="VN034_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWV60585.1"
                     /translation="MPQRKAKTSAFADEPQTLFTSTVLTQIRELIVTGELPPGTHLAA
                     EPIASRLGVSRTPVRNAFSVLLAEGLLEHSVNRGFSVREIPLRDVLGAIDMRAVIEAR
                     GCGMSIEYGWSHEELATLDERIAAGREIVERGDWSAQIERQWYEINRDIHAWIVRVGR
                     NPAIRSAMRMTLIYPIFGDVCRLCPSVSRYVPLRNRQIPPTVPDHIVESQAEHEAIAD
                     AIRADDAEGAERLMHQHVIAVKHRVLLAATRR"
     gene            6501..>7238
                     /locus_tag="VN034_08030"
     CDS             6501..>7238
                     /locus_tag="VN034_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60586.1"
                     /translation="MEPGPTRVIVSQRFFDNAAVRLLQGAGCEVVQAELPEGEADGNL
                     SHDQLVAMLDGAAGWIVGHARVTRELLAALPDLRIVSRRGVGYERVDIAAAKEFGKVV
                     CIAAGGNDASVADHTIALMLAVGHRFRETQARMIGGDWSILTGSDLYRKTVGVVGLGR
                     IGRSLVQRLGGFEARILVTAPTPDADYAARTGVEYVDLGTLLRESDYVTLHAPLTPET
                     RFLIDADALAAMKPGAFLINAARGGLVD"
BASE COUNT         1289 a         2321 c         2339 g         1289 t
ORIGIN      
        1 cggttcccac cccagccgca tccgctcgcg cgtcccgagc caccatgcca gcgccgcgag
       61 cgccgcgagt gcgatcaaca tcgccggcac gagcggcagg aagacatcat ggacaaggtc
      121 gaccttgacc gcggcggcag cccgcgccga cgggccgccc caaggcaaaa gattcatgat
      181 ggcgttgctg ctgccgagaa gcgttgcaag gatcagaggg ttcatcccga tggcccggta
      241 aagcggaagc atcgaagtga tgacgatcag cgcggtcgtc gtgccgtcgc catcgagcga
      301 cacaagcgtg gaaagcgcca tcgttccgat cgcgatacgg accggatcgt cgccgaccat
      361 ggcaagaatc cggttgacca gcggatcgaa aagccccgaa tccatcatca ccgcgaaata
      421 gagcacggcg aacgccagca tcgtcgcgat cggcgccaca tcgaggatgc ccttcagcat
      481 catgtccccg atctgtggac ccgcgccggc gagcaggccg ccggcgatag ggatcgcaat
      541 cagcgcggcg gcggccgaca tccgcttggt catgatcagg atgatgaaca gcaggacggt
      601 cgcgaaaccc agcagggcca atgtcatcgt ggatatcctc cggaacagtc gggccgtcgc
      661 gaccgctgca aaacgtcaat cgcgaaagcc ggggtcgatc cggtcgagtt tgcgcagcag
      721 cgcgggccat gcgagcagct cgctggccgc ttgcatgccc ggcgcgcgga cctggtcgcg
      781 aacgacgtcg gcgaccttgc cctcgggatc gtgcaggccg tcgcgtccgg cgccgagcat
      841 catcacctgc gcctcgcacg cgcgctcgag gaaatagatc cgcacgaaag catccgcaac
      901 gctgcgcccg accgccagcg tgccatggtt gcgcagcagc atggcctgtc tgtcgccaag
      961 gtccgcgacg atgcgctcgc gttccgccag attggtcgcg atgccttcat agtcgtgata
     1021 ggcgaggtcg tgatgtacga tcatcgcggt ctgcgtgtgc ggcaggagcc cgcagcgcat
     1081 cgccgatacg gcctgccccg ccggggtgtg cagatgcagc accgcggccg catccgcccg
     1141 cgccatatgg atcgccgagt ggatggtgaa acccgccgcg ttgacggggg cgggagcacc
     1201 atcgacctgc ttcccgtgga tgtcgatctt gacgaggctc gacgccgtga tctcctcgaa
     1261 catatgggta tagggattga tcaggaaatg atgatcgggg cccggcacgc gcgccgacag
     1321 atgggtaaag atcaggtcgt cccagccatg gagcgccacg aggcggtagg cggcggcgag
     1381 atcgacgcgt acggcccact cggcttcgtc gatatccccg cgcgcgccgg gagcgccttg
     1441 cgatgtcgtt gccatagtct ctttccccgc cggttctggc taccggcctc agccggcaag
     1501 ttcgacatcg agcagcggca catattgctg tcccgagtgc atgtcgcgtt cgaccatgcc
     1561 accctgcggc gtggtgcgcg gataggaaat cttgatcatc gacagatcgt cgaccgggat
     1621 ccgcttcacc atcgccgcct cggcgccata gagacgcgcg aagctgtcgg cagagagcgc
     1681 gggatcttgg tgatatttgg caaaattgtc gggcccgtcg aaaaatatgt cgaaggttac
     1741 ccagaagggg cccgcgtttt tcgagcggat ctcgtgacag acgtcgcgca gcttaaccat
     1801 gggccacctc cttgcgcgct tcgacggacc gggacgccag atcgacatat tcgatccgga
     1861 ccagctcgaa cgggtcgtcg agcgccacga tatggttgag ccggaactca tatacctgtc
     1921 cgcaagggac atcggccggg ctcagcatga agccatagct cggtatctcc ttgttcatca
     1981 cattgggaaa atggaagaaa tagggattac aggcgtgcgc gatcgcattg gccagctcct
     2041 gcgtatcggc ggtcgccacg aaaatcatgc cgatctcggg cggcggcggt gtgccggggc
     2101 gcaccggctt gccgtcgatg ccgttccacc cgtacaggcg gagcgagatg tggaattcgc
     2161 cgagcacctc ggcaggaaac gacttgcgcg tccggtcgta aagcgccgcc agcagcttgt
     2221 cgtggaagtt ctcgatgtcg ttcaggactt cggggtcgcg gatgccgacg agcatcatcg
     2281 tctgatactg ccccgcgctc gccccctcga gcttcatcga atagggtccg ggttcccatt
     2341 ccgacccgct caccgaaacg gtgcgatcgt ccttctgcgt atagcgagcg cgcgtcacgt
     2401 cgagcacgcc gccgggctcg gcgaggcgga aaggatcggt gttttcgtag agcatatgcg
     2461 ccgaaacgct gtgcaccgaa cagcgattgt catcgctgag cggcgcgacc tcgaatccgt
     2521 cgctcttgac ggtcagcatg acgcccgaac ccagcgtcgg attgaccgag cattgcaccc
     2581 cgcattcgcc cgtcttgccc gcgtgccacg ccggccccca cggcgcgccc ttccagatcg
     2641 gatagcatgc gaggaccgcg gtatcggtcg agcggccgcc gagaatgatg tcggccccgc
     2701 cgtcgagcgc cgcgatgaaa tgttcgaccc cgagcgccgc gacgatatgc tcgcactggt
     2761 cgacgatctc gtccgcgagc gggccgagcg gcggcaaggg gtggatgcgc ccttcggcgt
     2821 tcagccgctt gaccgtcgcc ttgtcctgct cgcaatggat gagcgcgatc ctgggcgcca
     2881 tagccagttc ggccgcgact tcttccacga tctcgcgggt ccattcgagg ttcagatcgc
     2941 ccccggcctg ccccgaggtg ccgatgatga tcggaatgcc gtgctcggcc tgcgccttca
     3001 tcaatatctc gaggtcgcgc ttgaccgcac cgcggctgtt cttcgacttg cccagcgcca
     3061 gataggcggc gccgctgtcg gtcgatccgg catcggacgc gatcacatgc gcgccctcgg
     3121 cgatcccgta tgcaatctcg ctttcgcgca ccccggcgcc gagcgagccg catggcacaa
     3181 gcactttgat cgggccggca aaagcgccgg cggcttgcgg ctgttctgtc acgctttacg
     3241 ctccttcatc atcaaatcgt cgtggccggc ggcgctcaat agcgccagcc gagcgtcgct
     3301 ccgtaggtcc gcggcgggtt gtaagtgccg aaattggcgc cgaggaacag cgtctgatgc
     3361 gtgcgcgtgc gctcgttggt cagattcttg ccccacagcg acagggtcca atgatcgccg
     3421 aacccggcga gtacgacacg cgcatcgacg aagtgcgtcg ccggccgaac ctcggggccc
     3481 tcgttgctgt tgtcgtcgta gatcaggctt tcatattgat agtcggcagc gaccgtcagt
     3541 tcggtgcggc cgccgagcgc cacggtatag gaaggcgaca gcgtcagctt gtgcttgggg
     3601 gtgcgggaga tgcggttgcc cgaccggtcg atcccctcgt cgtcgaactc atcgaagcgc
     3661 gcgtcggtat aggcataggc ggccgagagg ttaaggccat cgaagagacg cgcggtcagc
     3721 tgcatctcat atcccttgat cgtggccttg ccggcgttgt tggtgatgat cgagccgtcg
     3781 ggctgcgact gacgggtttg caggtcccgg taatcgagcc agtagagcgt gttgttccag
     3841 atcagccgcc catcgaaaaa ccggctcttc tgaccgatct cgtactgcac cgccgtttcg
     3901 ggatcgaacg ccgtcgccgc gtcgatcgcg ctcgacggcg tgtcctgaaa gccgccgctc
     3961 ttgaagccgc gcgacaccat cgcatagagg agatgatcgt cgttcggctt gaaattcagg
     4021 ccgccgcgat aggtgacgtc gtcccagctc ttctgcgtgg tgacgtcgaa gaactcctcg
     4081 ccgcgcagcg gcgccatgcc gcgcgtgttg gtgacgcggt atgtcttgtc gtcgcgcgaa
     4141 tagcgcacgc cgccgatgat gctgaagcgg tcgccgagcg gaatcgtcgc gtcgccgaac
     4201 agcgcgatgc tgtcgagccg cgcgtcctga tcgtaaatgt cggtgagcgg gatcggcgcg
     4261 acgaactcgc tgtcgaggtt gaaaatgtcg gaccggaccg tttcctgccg ataatggtaa
     4321 aagccgacga cccagtcgat cccgctgccg ggcagcgacg acaggcgcac ttcctgactc
     4381 gacagctccg accgctcgtc ctcgccgccg ctgatgtcga ccagtgaacg cgtcggattg
     4441 ctgccgtcga aatcgtagcg cgatccatat tcgagatcgc ggtacgaccc gaggaaggag
     4501 agcgtggcga agctcatatc ccaggcgatt tcgccgcgca cgccgatcgt gtcacgatcc
     4561 tgatagccgg cgaaggagcc ataggtgcgc ttgggatcgc gatcgaccgt ataggcggcc
     4621 gacaggggat cgtcgggatc gacgtcgaag atatatttgg ccggaccggt cgcacgatcg
     4681 cgggttccat cgagcgtgaa gtgcacgcgc agattgtcgg tcggctccgc gagaaactgg
     4741 aaccgcccgc tcagcgtgtc ctgatcctcg acgcggcggt cgatgacggg attatagaca
     4801 tatccgccgt gcgagcggta ggcgccgctg atccggatcg ccccgccccc accgccgaac
     4861 ggcaggttga cgaagccggc gccgtcgagc cggtcgtaat tgccataggt gaattcggct
     4921 gccgcatcga aaccgccgag ttcgggccgc ttggtcacga cgttgatcgc gccgccgacg
     4981 acgttgcgcc cgaacagcgt gccttgcggc cccttgagca cctcgatccg ttcgacgtcg
     5041 aaggcgtcga acgcgaccgc cgcagggcgt ccgagataga tctcgtcgag gaacacggcg
     5101 gcgctcggat ccccggcggc gcccttgtcg gacgatccga tcccgcgcac cgccagccgc
     5161 ggctgacttg ccgggaaagc gtcgaagctg aggccggggg tgcgggtcac gacatcgctg
     5221 acctgctgga tccgcgcctg ctggatttcc tcggccccga atacggtgac cgttaccggc
     5281 acatcctgca ggctctgggc gcggcgctgc gcggtgacga tgatcgcatc atcgtccgcc
     5341 ctggccgcac cgggcgcctc cgagccatcc tgtgcccgcg ccgcttgcgc attcgaaagc
     5401 gccagaacgg cggcgccgca aaataagatc gcacctcgca tagcatcctc cacttggacg
     5461 gccgtctgtc gcggcctgcc gccgctctaa tgatagattg gatgcaatat gtcaaatata
     5521 tggattcagt ttaacgagcg cgcgccgacc cacgcgcccg attccggtga aatcagcagg
     5581 gtttcagcgg cgcgtggcgg ctagaaggac gcggtgcttc accgcgatga catgctggtg
     5641 catgagccgc tcggctcctt cggcatcgtc ggcgcgaatc gcatcggcga tcgcctcatg
     5701 ttcggcctgc gattcgacga tatggtccgg aacggtgggc ggaatctggc ggttacgcag
     5761 cggcacatag cgcgacaccg acgggcacaa ccggcagaca tcgccgaaaa tcggatagat
     5821 gagtgtcatc cgcatcgcgc tgcggatggc gggattgcgc ccgacacgga cgatccaggc
     5881 atggatgtcg cggttgattt cataccattg ccgctcgatt tgggccgacc agtcgccgcg
     5941 ctcgacgatc tcgcgacccg ccgcgatccg ctcgtcgagc gtcgcaagtt cctcgtgcga
     6001 ccagccatat tcgatcgaca tgccgcaacc gcgcgcctcg atcactgcgc gcatgtcgat
     6061 tgcacccaaa acatcgcgca gcggaatctc gcgcaccgag aaccctctgt tgaccgaatg
     6121 ttcgagtaac ccttcggcca gcagaaccga aaaggcgttg cgcacgggcg ttcgcgacac
     6181 gccgagccgg ctcgcgatcg gctcggcggc cagatgcgtg cccggtggga gttcgccggt
     6241 cacgatcaac tcgcgaatct gggtgagcac agtcgatgtg aaaagcgtct gcggctcgtc
     6301 ggcgaatgcg gatgttttgg ctttgcgctg gggcatgatg gcgtgcgtta gcattcagcg
     6361 ggaccgccac gccaccaaaa tgttgcaaat tgaattcaat ctttgatatg attgattcgc
     6421 aaatttcatt tgtttcataa tagcggccga gctccctccc cgtcggatgg cgcaacacag
     6481 gccgatttgg gaggaaagtc atggaaccgg gccccactcg cgtcatcgtc tcgcaacgct
     6541 tcttcgacaa cgcggcagtt cggcttcttc agggggcggg atgcgaggtc gtgcaggccg
     6601 aattgccgga gggcgaggcc gacggcaatt tgtcgcacga ccagcttgtc gcgatgctcg
     6661 acggcgccgc gggatggatc gtcggccatg cgcgggtgac gcgcgaattg ctcgccgcgc
     6721 tgcccgacct ccggatcgtg tcgcgccgcg gcgtcggcta cgagcgcgtc gacatcgccg
     6781 cggcgaagga gttcggcaag gtggtgtgca tcgccgccgg cggcaacgac gcctcggtcg
     6841 ccgaccacac gatcgcattg atgctcgcgg tcgggcatcg cttccgcgaa acgcaggccc
     6901 ggatgatcgg cggcgactgg tcgattttga cgggctcgga cctctatcga aagacggtgg
     6961 gcgtcgtcgg actcggccgc atcgggcgca gcctcgtcca gcgcctcggc gggttcgagg
     7021 cgcgtatcct tgtcacggcc ccgacccccg acgccgatta tgcggcacgc accggcgtcg
     7081 aatatgtcga tctcggcacg ctgctgcgcg aaagcgatta tgtgaccctc cacgcgccgc
     7141 tcacgcccga gacgcgcttt ctgatcgacg ccgatgcgct cgcggcgatg aagccgggcg
     7201 cgttcctgat caacgccgcg cgcggcgggc tggtcgat
//
LOCUS       DATMSU010000293         2404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_109358,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000293 DATMSU010000000
VERSION     DATMSU010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109358"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VN034_08035"
     CDS             <1..1131
                     /locus_tag="VN034_08035"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWV60587.1"
                     /translation="DAERADLTARATAALQSGFATGYRALIAHLRAAEAKADATAGVW
                     KLPDGQAYYAAQLESYTTLPLKAADVHALGLREVASIHDAMRAVMKRVGFAGDLQAFF
                     AFMRSDPRFYHPDTDAGRAAYVAEADALLDEIRARQGELFAHLPKAPVEVRAIEAWRQ
                     KSAPKAHYRNPPQDGSAPGIFYINLADMKAQPRYQLAATLYHEAIPGHHVETCIAHEM
                     EGLPKFRRFASVAAFSEGWGLYSELLPKEMGLYADPYSDFGRLSLSLMRACRLVVDTG
                     IHSLKWTREQGIAYFDTNMPSSHYDNQREVERYIVIPGQATAYYIGMQKIIELRARAR
                     AKLGDRFDLRRFHDITLGAGPLPLPLLEERIDAWIAGGGQSA"
     gene            1128..2351
                     /locus_tag="VN034_08040"
     CDS             1128..2351
                     /locus_tag="VN034_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60588.1"
                     /translation="MKRLSAVLLALCSATAAHAAPPHYDVAAKFDPHGALDADVTITL
                     SEGEAEKAFLLSDRFTLRPMDLPAGVTLSVEPSTSPMPALNKYSFRFAAPATDPVKLR
                     FRYAGPILTEHDSGNRPLRPEGYELFIDHMWFPVGADIQTRFTVDATIDGLAPDLVVV
                     AQGDVTRTPTGVRIHRDFLDIDLPMVAMHGLQRAEAHGVEFYSRDLSTQLSGWYVKHA
                     EGAARYFEAMYGPLPRKVRMAQVWRERSMGYARTAYTVLSEGGRNTPDITEVNPGRYV
                     AHEIAHAWWMLASPLTDDFWLVESAAEYMSMRYVEHAFGPDELAKNIAAKRDATKDAG
                     PVMGHGRPNRIQLYGKGPLLLLDLEHRIGRPAMDRLMIAMAKEPAHTTPVFLKHLTVI
                     AGADAAREFAKALHT"
BASE COUNT          416 a          813 c          773 g          402 t
ORIGIN      
        1 gacgccgagc gcgccgacct caccgcgcgc gccaccgcgg cgctgcaaag cggctttgcg
       61 accggctacc gcgcactgat cgcgcatctg cgcgccgccg aggcaaaggc cgatgcaacc
      121 gccggcgtct ggaagcttcc cgatggtcaa gcctattatg cggcgcagct cgaaagctat
      181 acgacgctgc cgctgaaggc ggccgatgtc cacgcgctcg ggctgcgcga ggtcgcgagc
      241 atccacgacg cgatgcgcgc ggtcatgaag cgggtcggct ttgcgggcga ccttcaggct
      301 tttttcgcgt tcatgcgcag cgacccgcgc ttctatcacc ccgatactga cgccgggcgc
      361 gccgcctatg tcgccgaggc cgatgcgctg ctggacgaaa tccgcgcgcg gcagggcgaa
      421 ttgttcgcgc atctgccgaa ggcgccggtc gaggtccgcg cgatcgaggc gtggcgtcag
      481 aaatcggcgc cgaaggcgca ttaccgcaat ccgccgcagg acggatcggc gccgggcatc
      541 ttctacatca accttgccga catgaaggcg cagccgcgct accagctcgc cgcgacgctc
      601 tatcacgagg cgatccccgg tcatcatgtc gaaacctgca tcgcgcacga gatggagggg
      661 ctgcccaaat tccgccgctt cgcgagcgta gccgcctttt cggagggctg ggggctctat
      721 tcggaactgc tgccgaagga gatggggctc tatgccgatc cctattcgga tttcgggcgc
      781 ctttcgctgt cgctgatgcg cgcgtgccgt cttgtcgtcg acaccggcat ccattcgctt
      841 aaatggacgc gcgagcaggg catcgcctat ttcgatacga acatgccgtc gagccattac
      901 gacaaccagc gcgaggttga acgctatatc gtcatcccgg gacaggcgac cgcctattat
      961 atcgggatgc agaagatcat cgaattgcgc gcccgagcgc gcgcgaagct cggcgaccgc
     1021 ttcgatctgc gccgctttca cgacatcacc ttgggcgcgg ggccgttgcc cttgccgctg
     1081 ctcgaagaac gtatcgacgc gtggatcgcc gggggaggac aatcggcatg aagagactat
     1141 cagcggtcct gctggcgctc tgcagcgcga ccgcggcgca cgcggcgccg ccgcattacg
     1201 atgtcgcagc gaaattcgat ccccacggcg cgctcgacgc cgacgtcacg atcacgctca
     1261 gcgaaggcga ggccgaaaag gccttcctgc tttcggaccg cttcacgttg cggccgatgg
     1321 atctgccggc cggcgtcacc ctgtcggtcg aaccgtcgac ctcgccgatg ccggcgctca
     1381 acaaatatag cttccgcttt gcggcacccg cgacggaccc ggtcaaactg cgcttccgtt
     1441 acgcggggcc gatccttacc gagcatgaca gcggcaacag gccgctcagg cccgaaggct
     1501 atgagctgtt catcgaccat atgtggttcc cggtcggcgc cgacatccag acgcgcttca
     1561 ccgtcgacgc gacgatcgat ggactggctc cggatctggt ggtcgtcgcg cagggcgatg
     1621 tcacgcgcac gccgacgggg gtgcgcatcc accgcgactt cctcgacatc gacctgccga
     1681 tggtcgcgat gcacggactg cagcgcgccg aggcgcacgg cgtcgaattc tattcgcgcg
     1741 acctgtcgac ccagctttcg ggctggtacg tcaaacatgc cgagggcgcg gcgcgctatt
     1801 tcgaggcgat gtacggcccg ctgccgcgca aggtgcggat ggcgcaggtc tggcgcgaac
     1861 gctcgatggg ctatgcgcgc accgcctaca ccgtcttgtc ggaaggcgga cggaacacgc
     1921 ccgacatcac cgaggtcaac cccggccgct atgtcgcgca cgagatcgca cacgcttggt
     1981 ggatgctcgc gagcccgctg accgacgatt tctggctcgt cgaatcggcc gccgaatata
     2041 tgtcgatgcg ctatgtcgag cacgccttcg ggcccgacga actggcgaag aatatcgcgg
     2101 cgaagcgcga cgcgaccaag gacgcgggcc cggtgatggg gcatggccgc ccgaaccgca
     2161 tccagctcta cggcaagggg ccgctgctgc tcctcgatct cgaacatcgc atcggccgcc
     2221 ccgcgatgga ccggctgatg atcgcgatgg caaaggagcc ggcccacacg acaccggtct
     2281 ttctgaagca cctgaccgtg atcgccggcg ccgatgccgc gcgcgaattc gcaaaggcgc
     2341 tccacacatg atcgtctcgc gccgcgcgct gatcggcggc gcggccgcgt tcgcgtttgc
     2401 aggt
//
LOCUS       DATMSU010000294         1870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_33560, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000294 DATMSU010000000
VERSION     DATMSU010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33560"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1462)
                     /gene="prsR"
                     /locus_tag="VN034_08045"
     CDS             complement(86..1462)
                     /gene="prsR"
                     /locus_tag="VN034_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HWV60589.1"
                     /translation="MSGGGTGLRTLLVVEDDAGLQTQLKWAYEDYRVLVAGDHDAAIE
                     MLRAEEPEVVTLDLGLPPDPDGTREGFRTLKAILEAKPDTKVIVVSGHGERASALTAI
                     ANGAWDFYQKPIDIDELGLIVARAFHVRELEVENARLASQGAGDNRVLGGMITGAPEM
                     QKVARTIERVANLDVSVMLLGASGTGKELLARGLHEASGRRDGAFVAINCAAIPENLL
                     ESELFGHEKGAFTGAVKTTEGKIELAHGGTLFLDEVGDIPLPLQVKLLRFLQERTIER
                     IGGRKAIPVDTRIVCATHRDLDAMIAEQSFRDDLYYRLAEMVVKIPSLAERAGDAVLL
                     ARHFLHQYAPEMNPGVRGFAPDALQTIDEARWPGNVRELENRIKRAVIMADGKLVMRE
                     DLDMAGGGEEGDEAWLNLRSAREAADRVAIRRAMTQSEGNISLAAKLLGISRPTLYDL
                     LKQYRMQS"
     gene            complement(1459..>1870)
                     /locus_tag="VN034_08050"
     CDS             complement(1459..>1870)
                     /locus_tag="VN034_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV60590.1"
                     /translation="FVGCQAGLSAWAEAGAVSQIVQHLVANAIDASAPETPVQVVAVA
                     EQGRVRIDVIDQGCGMTREFIRDELFKPFVSTKDSGFGLGAFEALQIAQAMDGAIEVA
                     SEPGRGSNFTLWLPLADARGAAASDARVTKVGMT"
BASE COUNT          288 a          699 c          568 g          315 t
ORIGIN      
        1 gcgtgtccgg tccgccgccg ccgcacgccg cgagcagaag cgccgccacc agcggcgcgg
       61 ccgtccagaa gcggcgcagg atcattcagc tctgcatccg atattgtttg agcaggtcgt
      121 agagcgtcgg ccggctgatc ccgagcaatt tcgccgcgag cgagatattg ccctcgctct
      181 gcgtcatcgc gcggcggatc gcgacgcggt ctgccgcctc gcgcgcgctg cgcaggttga
      241 gccacgcctc gtcgccctct tcaccgccgc ccgccatatc gagatcctcg cgcatcacca
      301 gcttgccgtc ggccatgata acggcgcgct tgatgcggtt ttcaagctcg cggacattgc
      361 ccggccagcg cgcctcgtcg atcgtctgca aagcgtcggg cgcgaagccg cgcacgccgg
      421 ggttcatctc gggcgcatat tggtggagga aatgccgcgc gagcagcacc gcatcgcccg
      481 cccgctcggc gagcgagggg atcttcacca ccatctccgc gagccggtag taaagatcgt
      541 cgcgaaagct ctgctcggcg atcatcgcat cgaggtcgcg gtgagtcgcg cagacgatcc
      601 gcgtatcgac cggaatcgcc ttgcgcccgc cgatccgctc gatcgtccgt tcctgtaaaa
      661 agcgcagcag cttgacctgc agcggcagcg gaatgtcgcc gacctcgtcg aggaagagcg
      721 tgccgccatg cgccagctcg atcttgccct cggtcgtctt caccgccccg gtgaacgcgc
      781 ccttctcatg cccgaacaat tcgctttcga gcagattctc ggggatcgcg gcgcagttga
      841 tcgcgacgaa cgcgccgtca cgccgtccgc tcgcctcgtg cagcccgcgc gcgagcaatt
      901 ccttgccggt gccgctcgcg ccgagcagca tcaccgacac gtcgaggttc gcgacgcgct
      961 cgatcgtgcg cgcgactttc tgcatttcgg gcgcgccggt gatcatcccg ccgagcacgc
     1021 gattgtcgcc cgcgccctgg ctcgccagcc gcgcattttc gacctccagc tcgcggacgt
     1081 gaaaggcgcg cgcgacgatc agccccagct cgtcgatgtc gatcggcttc tgatagaaat
     1141 cccacgcccc gttcgcgatc gcggtcagcg cgctcgcacg ttcgccatgc cccgacacga
     1201 cgatgacctt ggtatcgggc ttggcctcga ggatcgcctt cagcgtgcgg aacccctcgc
     1261 gcgttccgtc gggatcgggc ggcaggccga ggtcgagcgt caccacctcg ggctcctcgg
     1321 cgcgcagcat ctcgatcgcg gcatcatggt cgcccgcgac gagcacgcga taatcctcat
     1381 aagcccattt gagctgcgtc tgcaggccgg cgtcgtcctc gaccaccagc aatgtgcgca
     1441 atccggtccc gcccccgctc atgtcattcc cactttcgtc acccgcgcat cgcttgccgc
     1501 cgcgccacgc gcatcggcga gcggcagcca cagggtgaaa ttgcttcccc gacccggctc
     1561 gctcgccacc tcgatcgcgc cgtccatcgc ctgcgcgatc tggagcgcct cgaacgcgcc
     1621 gagcccgaac cctgaatcct tcgtcgacac gaagggcttg aaaagctcgt cgcggatgaa
     1681 ctcgcgcgtc atgccgcagc cctggtcgat cacgtcgatg cgcacgcgcc cctgctcggc
     1741 gaccgcgacg acctgcaccg gcgtctcggg cgccgacgcg tcgatcgcat tcgccaccag
     1801 atgctggacg atctggctga ccgcgcccgc ctcggcccat gccgacaggc cggcctggca
     1861 accgacgaac
//
LOCUS       DATMSU010000295         7625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_156876,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000295 DATMSU010000000
VERSION     DATMSU010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7625
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156876"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..382
                     /gene="grxC"
                     /locus_tag="VN034_08055"
     CDS             122..382
                     /gene="grxC"
                     /locus_tag="VN034_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HWV60591.1"
                     /translation="MAQIEVYTKAFCPYCTRAKMLLDGKGADFREIDVTMDRAGFDAM
                     VERANGRRTVPQVFIDGKHVGGSDDLAALDAKGELDALIGAA"
     gene            386..847
                     /locus_tag="VN034_08060"
     CDS             386..847
                     /locus_tag="VN034_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HWV60592.1"
                     /translation="MIVFDLCCAAGDHRFEAWFASSDSFADQQARGLIACPICGDSAV
                     KKAVMAPRVGAKSNQASIVPVSKTTEPEPGPELVRKLLAGIAAKQAEMLPQSRWVGRD
                     FADAARAMHEGRAAEDLIHGQASPDEAQALRDDGIAAMPLLVPIVSPDAVN"
     gene            897..973
                     /locus_tag="VN034_08065"
     tRNA            897..973
                     /locus_tag="VN034_08065"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:931..933,aa:Arg,seq:cct)
     gene            1142..2890
                     /locus_tag="VN034_08070"
     CDS             1142..2890
                     /locus_tag="VN034_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWV60593.1"
                     /translation="MKRYSDVRPYVKEVLLALCFLSALPSIVNRSADLVGAVPHAGYL
                     LLLIMLWGGLLGAAYTPQAILRWTTAFLLAGAAYYMVVFERVTTEFMTYDAFINMLNS
                     AAFIGDALAQNRTAFVEAAPPALLLLAAIAMAPRRHLPLPRWLVAATPWLATFALTGL
                     LFLRGGDGARGQPPSFTPIAYLTLVGYENATGNMGKRQSVRLRQVRRPLSRNIILIVD
                     ESVAGQYLDINSPAGVPTPLAGVWPFARIYNYGLAASVTNCSIGSNVTLRYGGTRNDY
                     QRINGTQPSIWAYARKAGLRTVYIDSQRTGGALQNMMTDEERALIDKFVQFDKVPVEQ
                     RDRAAARELIRELADHRPKFIFVNKVGAHFPVHDKFPEAYLRYDPVLPRGRYRNISDT
                     GSRAGFAGGVEDWLRYRNSYRNTLLWNVGAFFETLRNQADFAHATMIYTSDHGQNLHE
                     RGNPGLSTHCSVDPAQEEGVVPLVVIDGNRGSSVDWHRDVAHNRNRSSHYMIFPTLLE
                     LMGYDPSAVEKRYGRALNEASHDPVSFNKLFNARLNRKPEWVTIDPASVVQPPRADNR
                     DSAHTRGARRHSTPTP"
     gene            3093..7544
                     /locus_tag="VN034_08075"
     CDS             3093..7544
                     /locus_tag="VN034_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60594.1"
                     /translation="MTISTFVFSNSTGPYAPSQTDGGVTAHDFTVSGPGATFDGTYLQ
                     LPAGTYLTAGMAGVTLPTDADPAKTQTPEEFTLEYIGILPATDSPLVSLYDYNDGEIS
                     LYAHWDTGRLRANFERSGTRAQIDSIGGLSMTAEERVGLRYVDDPSGAGGTITFLRNG
                     APLGASQPLAFKPRITPDAMLECNASLDNTANSGAQKLREIKLILGTQTGNPPGGGDG
                     DGDGKSSTFAFPNATGPYTPVQTDGGVTAQGFAISGSGATFDGTYLQLPAGTYLTAGN
                     AGITLPTNTDPAKTQTPEDITLEYVGILPATNSPLVSLYDYNDGEISLDAHWDTGRLR
                     ANFERSGTRAQIDSIGGLSLTAEERVGLRYVDDPSGAGGTITFLRNGVALGASQPLAF
                     KPRITPDAVLECNASLGNTANSGAQKVREIKLTLGTQDTNPPGGGDGTFTVYGEPGTR
                     FPLDIPVSGGAATYAVTIPSGLAVQERVASNILIDRASAYAFPNTGPNFAPAQTNDGV
                     TTNSLSMVGSGATFDGTYLRLPAGTYLTAGSLGIALPNSSDPAKTQTPVAIELEFTGI
                     LPAVSSPLVSLFDYDTGEFSLNSHWDTGRMRLTMQRDAQSIQIDSIGGLSTTAQEKLG
                     VRYDDDPAGAGGTVTFLRNGAPFGPSQAVAFKPRITPDAELEHNASLGNTTNSVAQKI
                     ADLSVKIKTAGQSYAFEPVTSGTISAARLETLHVDATGVSSPQAAKTISVTPAGGTAF
                     TVDVIVGPVAVAAGQAYRAVLENWSSGSRVDDPQVLVMTRPARQNCQFEDSFLRANQA
                     PWMECLPEGPVPTIGGIAYYCEAIRSGSYVQFQFGYDWTAAMMPDNPFGDPTGNDSYM
                     VPHKWRIEDQAGNVLATVQRPDGGPLNGTDIPRIFSGSYDGNGAAITNATDKWYPHGT
                     VRSGVIWRSGTPAAYDQTAINANLPRYDASVPYGSHTHFSSNGFDVRLFGSDGFNGFG
                     NTRVMPYQPTNHATLVPQAGVTNDPYKGSLYAASSLAAVASTWLRYTPFNQCGRSPIT
                     GPGGVRDDRCAIAEPVAAYMHDVASLRPHDGTARAQIAIDYLTAYASDPFHCFEGGRN
                     VPLFKGANAVRDIGLRDHYYGYGSGSRPANKSYYLQGGRQYNMADSYDPWSSKVPYGG
                     VAADKPYFGTNEIDLPHAHQFPHWGSLLWKSPEFAFLGHKLSDQARLYENWILSSPFG
                     DPNLFAERGAAWQFLHAALIWKTASRNSDRLYSRDEILNFVVQDFETFSAAHKTSNPG
                     FDNPPANVMVGGSIDDARAIYAATHRFGVCGLHDDDIAQHDFYIGYWLTALGIAERIG
                     FNAAVRARSATAGAVIDWLIAKHRQRIVGRINAAADTDCGTGTPYLLRIWSRASVTAA
                     GGNVASLPQGYANLAAVNGTNASWDVFQEGGSAGTRDGQAMDQLIAGPSILKNQLGQS
                     GTDLDQAATTAASWRSEKVTEQNLLGPNGAGTSWFRYLQVVHNPAIS"
BASE COUNT         1394 a         2476 c         2386 g         1369 t
ORIGIN      
        1 gcggcgcgcg tgtcggcgct gacctgggcg cgcgtcgtcc ccgacgcggt ggggggcaac
       61 atatttgact ttgcttcgtt ggattccgat atgcacagcg aaaggatgac aggatagccg
      121 catggcccag atcgaagttt atacgaaggc tttctgcccc tattgcacgc gcgcgaagat
      181 gctgctcgac ggcaaggggg ccgatttccg cgaaatcgat gtgacgatgg accgcgcggg
      241 gttcgacgcg atggtcgagc gcgcgaacgg acgccgcacc gtgccgcagg tcttcatcga
      301 cggaaaacat gtcggcggca gcgacgacct cgccgcactc gatgcgaagg gcgagctcga
      361 cgcgctgatc ggggccgcct gacccatgat cgtgttcgat ctttgctgcg ccgccggcga
      421 tcaccggttc gaggcttggt tcgcgagcag cgacagcttc gccgatcagc aggcgcgcgg
      481 cctgatcgcc tgtccgatct gcggcgacag cgcggtgaag aaggcggtga tggcgccgcg
      541 ggtgggcgcg aagtccaatc aggcctcgat cgtgcccgtt tcgaaaacga ccgagcccga
      601 acccggcccc gaactggtgc gcaagctgct cgccggcatc gcggcaaaac aggccgaaat
      661 gctgccgcaa tcgcgttggg tcggtcgcga tttcgccgat gccgcccgcg cgatgcacga
      721 agggcgcgcg gccgaggacc tgatccacgg ccaggcctcg cccgacgagg cgcaggcgct
      781 tcgcgacgac gggatcgccg cgatgcccct gctggtgccg atcgtgtcgc ccgacgcggt
      841 caattgatcc aagccgattg acgctccgcg cgctccgccg ctacacgagc ggcggcgcac
      901 ccgtagctca gcaggataga gcatcagatt cctaatctgg gggccactgg ttcgaatcca
      961 gtcgggtgca ccatttccgt tcgatgatgg gcaccgggaa atacggctgg gctccggtcg
     1021 ctgcgaaaca gcatcgaata gagttggata tatcgtcgcc acggcccttg ccgacaacaa
     1081 cttcaattta agatcctttt agggggaagg ggaatagcat agggcatgtt ccgggggtga
     1141 catgaagcga tattccgatg tgcggcctta cgtcaaagag gtgttgctgg cactttgttt
     1201 tctttcggcg ttgccttcca tcgtcaatcg ctctgccgat ctcgtcggcg ctgtcccgca
     1261 cgcaggctat ctgcttctcc tcatcatgtt gtggggcggg cttcttggag ccgcctacac
     1321 tccgcaagca attctgcgct ggacgaccgc gttcctgctg gctggagcgg cctattacat
     1381 ggtcgtgttc gagcgggtga cgaccgagtt catgacctat gacgccttca tcaacatgct
     1441 gaactccgcg gcgttcattg gcgatgccct ggcgcagaat cggacggcat ttgtcgaggc
     1501 ggcgcctccc gccctactcc tgctggcagc catcgcaatg gccccgcggc gacaccttcc
     1561 gctgcctcga tggctggtcg ccgcgacgcc ttggctggcg acattcgcgc tgacgggact
     1621 gcttttcctt cgcggcggcg acggtgcgcg cggacagccg ccgagcttca cgccgatcgc
     1681 ttacctgacg cttgtcggat atgagaacgc gacagggaac atgggcaagc gccagtccgt
     1741 tcgccttcga caggtccgcc ggccgctcag ccggaatatc atcctcatcg tcgatgaaag
     1801 cgtcgccggc caatatctgg acatcaattc gccggcaggc gtgcccacgc cactcgcggg
     1861 cgtctggcca ttcgcccgca tttataacta cggtctcgcc gcgtcggtga ccaattgcag
     1921 catcggcagc aacgtcaccc ttcgctacgg cggcacgcgc aatgactatc agcggatcaa
     1981 cggcacgcag ccgtcgatct gggcctatgc ccgcaaggcc gggctgcgca ccgtgtatat
     2041 cgatagccag cgaaccggcg gcgcgctgca aaacatgatg acggatgaag aacgtgcgct
     2101 gatcgacaag ttcgtgcaat tcgacaaggt gcccgtggaa cagcgcgacc gggcggcggc
     2161 gagggaactc atccgtgaac tggccgatca tcgaccgaag ttcattttcg tgaacaaggt
     2221 cggtgctcat ttcccggttc atgacaagtt tccggaggcc tacctgcgct atgatccggt
     2281 tctgccacgc ggacgatacc ggaatatttc cgataccggc agccgcgcgg gcttcgccgg
     2341 cggagtggag gactggcttc gctaccgcaa ttcttatcgc aatacgttgc tgtggaacgt
     2401 gggggcgttc ttcgaaacgc ttcgcaatca ggccgatttc gcgcatgcga cgatgatcta
     2461 cacgtccgac catggccaga atctgcatga gcggggcaat cccggacttt ccacccattg
     2521 tagcgtcgat cccgcccagg aagaaggcgt cgttcctctg gtggtgatcg acggcaaccg
     2581 gggttcaagc gtggattggc accgggatgt cgcgcataac cgcaaccggt cgagtcacta
     2641 tatgattttc ccgacgctgc tggaactgat gggttatgat ccgagcgccg ttgaaaagcg
     2701 ctacggacgg gcgctgaacg aagcttcgca cgatcccgtg agcttcaaca agctgttcaa
     2761 tgcgcggctc aatcgcaagc cggagtgggt cacgatcgat ccggcaagtg tcgttcagcc
     2821 gccacgcgcc gacaaccgcg acagtgcgca cacaagaggg gctaggcggc actcgacccc
     2881 aactccctga gctggtcacc ggcaacacgg ccaacgccgg atacttacat tccgtgaagc
     2941 tccggagcgt ttccgactgt ccgcggcaga agaattgcgc ctgagtacga aataggattc
     3001 ttcctatctt tcctacaata tttgttccct ttacgttcct tctcgaatcg gcttcgtctc
     3061 tatctaaccc gctgattcga aagaggatca ccatgaccat ttcgactttt gtcttttcga
     3121 actccaccgg cccttatgcg ccctcccaga ccgatggcgg cgtgaccgcg catgatttta
     3181 ccgtctcggg tccgggggcg acctttgacg gcacctatct ccaactgccg gcgggcactt
     3241 acctgaccgc aggaatggcg ggagtcaccc tgccgaccga tgccgacccg gcgaaaaccc
     3301 agacccccga agaattcacc ctcgaatata tcggcatcct gccggccacc gacagcccgc
     3361 tcgtctcgct ctatgactat aacgacggcg agatttcgct ttacgcgcat tgggacaccg
     3421 gccgtctgcg cgccaatttc gaacggagcg gcacgcgggc gcagatcgac agcatcggcg
     3481 gtctttcgat gaccgccgag gaacgcgtcg ggctgcgcta tgtcgacgat ccgtcggggg
     3541 cggggggcac gatcaccttc ctgcgcaacg gcgccccctt gggggcgagc cagccgctcg
     3601 ccttcaagcc gcggatcacc cccgacgcga tgctcgaatg caacgcctcg ctcgacaaca
     3661 cggcgaacag cggcgcccag aagctgcgcg agatcaaact catcctcggc acccagacgg
     3721 ggaatcctcc cggcggtggc gacggcgacg gcgatggcaa gagctcgacc ttcgccttcc
     3781 ccaatgccac cggcccctat acgccggtgc agaccgatgg cggcgtgacg gcgcagggct
     3841 tcgccatatc gggttcgggt gcgaccttcg acggcaccta tctgcaattg ccggcgggca
     3901 cttatctgac cgcggggaac gccggcatca cgctgccgac gaacaccgat cccgccaaga
     3961 cgcagacgcc cgaggacatc accctcgaat atgtcggtat cctgccggcc accaacagcc
     4021 cgctcgtctc gctttacgat tataacgatg gcgagatttc gctcgacgcg cattgggaca
     4081 ctggtcgcct tcgcgccaat ttcgagcgga gcggcacacg ggcgcagatc gacagcatcg
     4141 gcggcctttc gttgaccgcc gaggagcgcg tcggactgcg ctatgtcgac gatccgtcgg
     4201 gcgcgggcgg cacgatcacc ttcctgcgca acggcgtcgc gctgggagcg agccagccgc
     4261 tcgcgttcaa gccgcgcatc acccccgatg cggtgctcga atgcaacgcc tcgctcggta
     4321 acacggcgaa cagcggcgcg cagaaggtcc gcgagatcaa gctcaccctc ggaacgcagg
     4381 acaccaatcc tcccggcgga ggcgacggca ccttcaccgt ttatggcgag cccggaacgc
     4441 gttttccgct cgatatcccg gtttcgggcg gcgccgcgac ctatgccgtg acgatcccgt
     4501 cggggctggc ggtgcaggaa cgtgtcgcgt cgaacatact catcgaccgg gcgtcggctt
     4561 acgctttccc gaacaccgga ccgaatttcg cgccggccca gaccaatgac ggggtcacga
     4621 ccaattcgct gtcgatggtc ggttcgggtg caaccttcga cggcacctat ctgcggctgc
     4681 ccgcgggaac ttacctcaca gccgggtcgc tcgggatcgc cctgccgaac agctcggatc
     4741 cggcaaaaac ccagacgccc gtcgcgatcg aactcgaatt cacgggtatc ctgcccgcgg
     4801 tatcgagccc gctcgtttcg ctgttcgact atgacaccgg cgaattttcg ctgaacagcc
     4861 attgggatac gggacgtatg cgcctgacga tgcagcgcga cgcgcagagc atccagatcg
     4921 acagcatcgg cggactttcg accacggcgc aggaaaagct cggcgtgcgt tacgacgacg
     4981 atccggccgg cgccggcggg acggtcacct tcctgcgcaa cggcgcgccc ttcggcccta
     5041 gccaggcggt ggcgttcaag ccgcggatta cgcccgacgc cgaactcgag cataacgcct
     5101 cgctcggcaa cacgaccaat tcggtcgcgc agaagatcgc cgacctgtcg gtgaagatca
     5161 agacggcggg gcaatcctat gccttcgagc cggtgacatc gggaacgatc tcggccgcgc
     5221 ggctcgaaac gcttcatgtc gatgcgaccg gtgtatcgtc gccgcaggcg gcaaagacca
     5281 tctcggtgac cccggcgggc ggcacggcct ttaccgtcga tgtcatcgtc ggcccggtcg
     5341 ccgtggcggc ggggcaggcc tatcgcgcgg tgctcgaaaa ctggagcagc ggcagccgcg
     5401 tcgacgatcc gcaggtgctg gtgatgacgc gcccggcgcg ccagaattgc cagttcgagg
     5461 acagcttcct gcgcgccaat caggcgccgt ggatggaatg ccttcccgaa ggccccgtcc
     5521 cgacgatcgg cggcatcgcc tattattgcg aggcgatccg gtcgggcagc tatgtccagt
     5581 tccagttcgg ttacgactgg acggcggcga tgatgcccga caatccgttc ggcgatccca
     5641 ccggcaatga cagctatatg gtgccgcaca agtggcgcat cgaggatcag gcgggcaatg
     5701 tgctggcgac ggtgcagcgc cccgatggcg gaccgctcaa cggcaccgac attccgcgga
     5761 ttttctcggg cagctatgac ggcaacggcg ctgcgatcac caacgcgacg gacaaatggt
     5821 acccgcacgg caccgtccgc tcgggggtga tctggcgctc gggcacgccg gccgcttatg
     5881 accagacggc gatcaatgcc aatctgccgc gttacgacgc ctcggttccc tatgggtcgc
     5941 atacccattt ttcgagcaac ggcttcgatg tccggctgtt cggatcggac gggttcaacg
     6001 gcttcggcaa cacgcgggtc atgccgtatc agccgaccaa ccatgcgacg ctggttccgc
     6061 aggcgggggt gacgaacgat ccctacaagg gctcgctcta tgccgcatcg tcgctcgcgg
     6121 cggtggcatc gacgtggctg cgctatacgc cgttcaacca gtgcggccgc tcgccgatca
     6181 caggcccggg cggcgtgcgc gacgaccgct gcgcgatcgc cgaaccggtt gcggcctata
     6241 tgcacgacgt cgcctcgctg cgtccgcacg acggcacggc gcgcgcgcag atcgcgatcg
     6301 attatctgac cgcctatgcc agcgatccgt tccactgttt cgagggcggg cgcaacgttc
     6361 cgctgttcaa gggggcgaat gcggttcgcg atatcgggct gcgcgaccat tattacggct
     6421 atggatcggg ttcgcgcccc gcgaacaaat cctattacct ccagggcggc cgccaatata
     6481 atatggcgga cagctatgat ccctggtcgt cgaaggtgcc ctatggcggg gttgcggcgg
     6541 acaagcccta tttcgggacc aacgagatcg acctgccgca cgcccaccag ttcccgcact
     6601 ggggatcgct gctgtggaaa tcgcccgaat tcgccttcct cgggcacaag ctcagcgatc
     6661 aagcgcggct ttacgagaac tggatcctct cgtccccgtt cggcgatccc aacctgttcg
     6721 ccgagcgcgg cgcggcgtgg cagttcctgc acgcggcgct gatctggaaa accgcgtcgc
     6781 gcaattcgga ccgcctctat tcgcgcgacg aaatactgaa cttcgtcgtt caggatttcg
     6841 agacgttcag cgcggcgcac aagacttcga atccggggtt cgacaatccg ccggccaatg
     6901 tgatggtcgg gggcagcatc gacgacgcgc gggcgatcta tgccgccacg caccggttcg
     6961 gtgtgtgcgg cctgcacgat gacgatatcg cccagcacga tttctacatc ggatactggc
     7021 tgaccgcgct cgggattgcc gagcggatcg gcttcaacgc cgccgtccgc gcgcggtcgg
     7081 cgacggcggg cgcggtgatc gattggctga tcgccaagca tcgccagcgc atcgtcggcc
     7141 gtatcaatgc cgcggccgac accgactgcg gcacgggcac gccctatttg ttgcggatct
     7201 ggagccgcgc cagcgtgacc gcggcgggcg gcaatgtcgc gagccttccg cagggttatg
     7261 cgaaccttgc ggcggtcaac ggcaccaacg cgtcgtggga tgtgttccag gagggcggga
     7321 gcgccggcac ccgcgacgga caggcgatgg atcagctgat cgccgggccg tcgatcctca
     7381 agaaccagct cggccagtcg ggaaccgacc tcgaccaggc ggcaaccacc gcggcgagct
     7441 ggcgcagcga gaaggtcacc gagcagaatc tgctcggtcc gaacggcgcg gggacaagct
     7501 ggttccgcta tcttcaggtc gtgcacaatc cagcgatctc ctgatgagct ggatcgcatg
     7561 agggacgcgc cggccccatt cggcccggac tgattgcgtg aagggcggat cgtcgttgac
     7621 gatcc
//
LOCUS       DATMSU010000296         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_62460, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000296 DATMSU010000000
VERSION     DATMSU010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62460"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="VN034_08080"
     CDS             complement(<1..1202)
                     /locus_tag="VN034_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWV60595.1"
                     /translation="MTQSPDRAAIRAPLRTLARRSEADIVADLRTALAASSASVDAVT
                     RRGLELIRKAKADGERETLVAQLMNRYRLSTEEGVVLMCLAEALLRVPDNATANALIR
                     DKIAGRHWAEGDDDDSPLVVALSARGLSLGSATLMLDAMGSKANPLSLLKSMIRRSGE
                     PVIRQAALAAMKLLGQQFVMGETIDAAVKRADKEKSELASFDMLGEAARTAEDAQRYF
                     DSYAAAIARIGKGAKPGDPHANHGISIKLSALHPRYEYLQRARVTGELIPRVIELAKA
                     ARAVNIPLMIDAEESDRLEPHMDVFAALIDAGIADGWTGLGIVIQAYQKRAPAVIDWL
                     ANRARARGVKLSMRLVKGAYWDTEIKRAQTLGLGDFPVFTAKLHTDLNYMRCAQLLSD
                     CQDCIYPA"
     gene            1300..1770
                     /locus_tag="VN034_08085"
     CDS             1300..1770
                     /locus_tag="VN034_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV60596.1"
                     /translation="MKNSSSTVELDEFDRKILAILGRDGRITFTELALQVGLSKTPCQ
                     QRVKRLVDSGLITGFRAIVDPAKVGLDHVAFTEVKLSDTREEALRQFNNAVRRIPEVE
                     ECHMIASSFDYLLKVRTPDIRRYRIVLGEKISSLPHVASTSTFVAMETILESAR"
     gene            1817..1890
                     /locus_tag="VN034_08090"
     tRNA            1817..1890
                     /locus_tag="VN034_08090"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1849..1851,aa:Gly,seq:ccc)
     gene            complement(2009..>2240)
                     /locus_tag="VN034_08095"
     CDS             complement(2009..>2240)
                     /locus_tag="VN034_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60597.1"
                     /translation="GCEFLTPVPGSAISAALLKAPHDDDAADDAGASAAADFGELYSR
                     RPSSAVLFTALTALFSAVILLFIIALTSLTFS"
BASE COUNT          384 a          738 c          718 g          400 t
ORIGIN      
        1 aacgccggat agatgcaatc ctggcagtcg ctcagcagct gggcgcagcg catatagttg
       61 agatcggtgt gcagctttgc cgtgaacacc gggaagtcgc cgagccccag cgtctgcgcg
      121 cgcttgatct cggtgtccca ataggcaccc ttgacgaggc gcatcgacag tttgaccccg
      181 cgcgcccgcg cgcggttcgc aagccagtcg atcaccgccg gcgcgcgctt ctgatacgcc
      241 tgaatgacga taccgagccc ggtccagccg tcggcgatcc ccgcgtcgat cagcgccgcg
      301 aacacgtcca tatgcggctc gagccggtcg ctttcctcgg cgtcgatcat cagcgggatg
      361 ttcaccgcgc gcgccgcttt cgcgagttcg atcacgcgcg ggatcagctc gcccgtcacg
      421 cgcgcgcgct gcagatattc gtagcgcggg tggagcgccg acagcttgat cgaaatcccg
      481 tggttggcat gcgggtcgcc gggcttcgcg cctttgccga tgcgtgcgat cgccgcggca
      541 tagctgtcga aatagcgctg cgcatcctcg gcggtgcgcg ccgcttcgcc gagcatgtcg
      601 aaactcgcga gttcggactt ttccttgtcg gcgcgcttga ccgcggcgtc gatcgtctcg
      661 cccatcacga attgctggcc gagcagcttc atcgcggcga gcgccgcctg acggatcacc
      721 ggctcgcccg agcggcggat catcgatttg agcaggctca gcgggttcgc cttgctgccc
      781 atcgcatcga gcatcaacgt cgccgagccg agcgacaggc cccgtgccga caaggcgacg
      841 accagcggac tgtcgtcatc gtcgccctca gcccagtgac gcccggcgat cttgtcgcgg
      901 atcagcgcgt tggcggtcgc attgtcggga acgcgcagca gcgcctcggc gaggcacatc
      961 agcacgacgc cctcttcggt cgacaggcga tagcggttca tcagctgcgc gacgagcgtc
     1021 tcgcgctcgc cgtcggcctt cgccttgcgg atcagttcga gcccgcgccg cgtcaccgca
     1081 tcgacactgg cggacgaggc cgcgagggcg gtccgcaggt cggcgacgat gtcggcttcg
     1141 ctgcggcggg ccagggtgcg taggggtgcg cggatggcgg cgcggtcggg gctttgggtc
     1201 atggcgccct ttatcatatt gcgacaagtc gatccgactt gtttacgagc atttcatata
     1261 cgaattgacc aagtttcgga ttgatcctag tcgattcaca tgaaaaacag ctcctcgaca
     1321 gtcgaattgg acgaattcga tcgcaagatc ctcgcgattc tgggccgcga cggacggatc
     1381 acctttaccg agctggcgtt gcaggtcggc ctgtcgaaaa ccccctgcca gcagcgcgtc
     1441 aagcgcctcg tcgacagcgg gttgatcacc ggcttccgcg cgatcgtcga tccggccaag
     1501 gtcgggctcg accatgtcgc cttcaccgag gtcaagctgt ccgacacgcg cgaggaggcc
     1561 ttgcgacagt tcaacaatgc ggtgcggcgg attcccgagg tcgaggaatg ccatatgatc
     1621 gcgagcagct tcgactacct cctgaaagtc cgcacccccg acatccgccg ctatcgcatc
     1681 gtgctcggcg aaaaaatctc gagcctgccg cacgtcgcga gcacgtcgac cttcgtcgcg
     1741 atggagacga tcctcgaatc ggcgcgctga aggattgacc gccgcgccgg aaggcggcaa
     1801 aggccggaaa cggcgcgcgg gtgtagctca atggtagagc agaagcttcc caagcttacg
     1861 acgagggttc gattcccttc acccgctcca gtttttcggt atcgtaacga gacgacggcc
     1921 tccttcgggc agggcgtttg gctgccgatg ttcgcttcgc ctccatctct tcatcgccgc
     1981 gcacgccatg cggaattcgc gcgcaggatc aggagaaggt cagcgaggtg agggcgatga
     2041 tgaagagcag gatcaccgcg ctgaacagcg cggtcagcgc cgtgaacagc accgcgctcg
     2101 acggacgccg cgaataaagt tcgccgaaat cggccgccgc gctcgcaccg gcgtcatcgg
     2161 ccgcgtcgtc gtcgtgcggc gccttgagca gcgcggcgct gatcgcgctc cccgggaccg
     2221 gcgtcagaaa ctcgcacccg
//
LOCUS       DATMSU010000297        13391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_185203,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000297 DATMSU010000000
VERSION     DATMSU010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 13391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13391
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185203"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1095
                     /locus_tag="VN034_08100"
     CDS             85..1095
                     /locus_tag="VN034_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HWV60598.1"
                     /translation="MSAPRLVHVDDSLPGISRARSGVGWRYRDAKGKIIRDRDEIDRL
                     NAIALPPAYEDAWFCPAANGHILATGYDARGRKQYRYHPDFRMAREADKYDRCAAFGH
                     ALPLLRARLEDDLNRRTLCSERVVAAVVRLLDLAALRVGNEQYVAANKSFGATTLRQR
                     HAKLSGKTLRLKYIAKGGAKREVTISDRSLTTLVRRLQDLPGQNLFQYEDGGDLCVVG
                     SDDVNAYIREAMGDEFSAKHFRTWSASVEAFAFLYDAPEPPTLKAMLEHVASRLGNTP
                     AIARKAYVHPAMLAAAQAREDFASAIGNLPRATRYLSRYERGFLTYLEQAPAAAVLLQ
                     SA"
     gene            1118..2323
                     /locus_tag="VN034_08105"
     CDS             1118..2323
                     /locus_tag="VN034_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWV60599.1"
                     /translation="MNFSILAAATTAAPKAAPAKAAATDPLADLQRYWDMSLAWVESH
                     WLQIGIAIGVGLAIYFLLSMVRRFALKYAKSAEGDLSLTHIAGRVIHKTKSSVLAIIA
                     IRMVASYAQPPAVIMHMIQFVFTVAVVLQVAIWAREIVLGLIQRRAAEGNNETLSNAM
                     GIIRLLISIALFAIAAIVILDNMGVNVTGLIAGLGIGGIAIGLAAQGIFSDLFASLSI
                     IFDRPFRVGETIKYDMSTATVERIGLKSTRLRSLNGELLVISNTNLLSKEITNFAHLH
                     RRRITFKIGVIYQTTPAMLRDLPPLLEEQVVAGGQEFIRSSFLTFGPSSLDFELIFDV
                     FSDDYDVVAAARTDIAIRVFDAMAKSGYEFAYPTQTTFTAAPDGTMIMPYSESPKPKA
                     AAAKAAKPG"
     gene            2419..2877
                     /locus_tag="VN034_08110"
     CDS             2419..2877
                     /locus_tag="VN034_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWV60600.1"
                     /translation="MATITPQELQTKVGEHLGTSDWVLVDQEMINKFADATGDHQFIH
                     IDEEKAKLTPFGGTIAHGFLTLSLMPLLGASTDGPKVGGVKMGVNYGCNKVRFLSPVR
                     SGKRVRSHVKLLELEEKRPGQWQQTNEVTVEIEGEEKPALIAEWISQFFV"
     gene            2932..4113
                     /locus_tag="VN034_08115"
     CDS             2932..4113
                     /locus_tag="VN034_08115"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWV60601.1"
                     /translation="MRDAVIVSTARTPLTKAARGSFNNTLAPTLGAHAVRAAVERAGI
                     EGGEIDDVVFGAAMQQGSQTMNVARLIALRAGLPVTVPGMSIDRQCSSGLMTIATAAK
                     QIIVDRQDIAVAGGIESISKVSGSGKVFIETDAELIAMHKDTYMPMIGTAEVVAKRYN
                     ISREAQDEYSFQSQQRTAAAQAAGKYDDEIVACNATMAVVNKETKEVSFQDVVANKDE
                     CNRPDTTLEGLSSLKPVMGEGFSITAGNASQLADGASACVVMEAKVAEKKGLQPLGRY
                     VGMAVAGTEPDEMGIGPVFAIPKLLERFELKMDDIGLWELNEAFAVQVLYCRDKLGIP
                     NELLNVNGGSISIGHPFGMTGARCVGHALIEGKRRGVKYAVVTMCIGGGQGAAGLFEV
                     F"
     gene            4113..4697
                     /locus_tag="VN034_08120"
     CDS             4113..4697
                     /locus_tag="VN034_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWV60602.1"
                     /translation="MRLAAPRIEPVDPDRLDADQRAALEPFLASDGGKVGGGKILNIF
                     RTLAHAPKALTAFLGWGNYILSKRNALSPRNRELVILRTGFNCRSGYEWTQHKRIGLD
                     CGLTEDEIARIKAGPDANGWNELDRAMLRATDELTTSHFVTDTSWAALAPLGDKGRMD
                     LVMTVGQYTQVSMILNSFGIQVEDGWDVDPDLRV"
     gene            4718..5008
                     /locus_tag="VN034_08125"
     CDS             4718..5008
                     /locus_tag="VN034_08125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWV60603.1"
                     /translation="MSNIGIIATLRVQPGKEEAFEGVFAELAAAVRANEAGNSYYKLF
                     RTAAAGVYKVMECYDDEAAVETHRASDHFRTLGAKLGPCLAGAPEIETLSAA"
     gene            complement(5158..5628)
                     /gene="soxR"
                     /locus_tag="VN034_08130"
     CDS             complement(5158..5628)
                     /gene="soxR"
                     /locus_tag="VN034_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HWV60604.1"
                     /translation="MHRTDLIAIGELAARTGVAVSAIRFYEAKGLIEALRTGGGQRRF
                     LRADIRRVSFILIAQQMGLSLEEIGDELARLPAGRTPNGADWTRISTGLRTRIDAQIA
                     ALERTRELLGNCIGCGCLSLKKCGLYNREDKAAQRGAGPRYLIGDRAGEIAEIG"
     gene            5715..6110
                     /locus_tag="VN034_08135"
     CDS             5715..6110
                     /locus_tag="VN034_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV60605.1"
                     /translation="MTHPFIEHVNLTVSDPDRTAGILSAIFGWHERWRGPARDGGRTI
                     HLGSDAAYIALYTGPDGQHADARYPKGEPLNHIGVQVEDLDTIEMRVKAVGLTPFNHG
                     DYEPGRRFYFFDPDGIEYEVVSYAEPRRR"
     gene            6176..6640
                     /locus_tag="VN034_08140"
     CDS             6176..6640
                     /locus_tag="VN034_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWV60606.1"
                     /translation="MSDDIKQQFWKALADSPYVMLGATGEREHHIPMNAQLDKDAHGA
                     FWFFTATDNRLAAGGPAMAQFAAKGHDLFACISGTLVHENDRTVLDKLWNNGIAAWYP
                     GGKDDPKLVLLRFDLDDAEIWTADPGIKGMFKLATGMTMKDGDLGKHAEVAL"
     gene            complement(6643..7635)
                     /locus_tag="VN034_08145"
     CDS             complement(6643..7635)
                     /locus_tag="VN034_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HWV60607.1"
                     /translation="MAIQLKSARVERWAVAGEFVISRGAKTHVDVVVAEIEGEGAVGR
                     GEGTPIYYLGEDAAGCRDQILLAAPHIAEMDAAEARAAVQLLMPAGAARNALDCALWD
                     LEARQRGLRLWQLAGCDGAPTSRVTAYTISLGTPGAMAEQAATAAADGYRLLKIKLTG
                     EDDRLRVAGVRQGAPEARLIVDANESWGEIDILSEAEALADMGVEMIEQPVPVGADAL
                     LDPIYAPLPFIADESCQTAADVARIGPFYDGVNIKLDKAGGLTEALRIADAADAAGLS
                     IMAGCMLSTSLAIAPAFVLAQRAKWVDLDGPALLARDREGGFEFREGRIGPPVI"
     gene            7660..8070
                     /locus_tag="VN034_08150"
     CDS             7660..8070
                     /locus_tag="VN034_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HWV60608.1"
                     /translation="MSGKRLSGKSLSERIGDLGHRLAIEAHAAWLAARDPRVPMLARA
                     LAVGVAAYALSPIDLIPDFIPVLGWLDDLVIVPLGLLVVRRLIPAPLWAELHAAAEAA
                     SERPSSRAGMAFILLLWAGLLYIVYWAVRTSPWH"
     gene            complement(8087..9307)
                     /gene="phaZ"
                     /locus_tag="VN034_08155"
     CDS             complement(8087..9307)
                     /gene="phaZ"
                     /locus_tag="VN034_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HWV60609.1"
                     /translation="MLYHAFEIQKNWLAGASALATAGAQVMQHPANPLGYFGGSPMFA
                     SALEVFAHAAAPRGKPGFELYETIVDGETVRVTEVVEARKPFGQLKHFKHKGTKDAPK
                     LLIVAPMSGHYATLLRGTVERMLPGHDVWITDWRDARNVPLEAGRFDLDDYVDYLISW
                     LEHIGPGAHVLAVCQPSVPSLAAAAIMAANKHKCRPRTLTMMGGPIDTRKAPTAVNDH
                     AMTRPHAWFQENVIATVPAYYPGAGRRVYPGFLQLAGFMSMNLGNHMMSHWEMFKHLV
                     DGDGESADKTKEFYDEYRAVCDMTAEFYLQTVDIVFQRHLLPKGEMTHRGQTVDVGAI
                     EDIAILAIEGERDDISGIGQTKAALTLAKALPADRKKYLMAKGVGHYGIFNGRKWREE
                     IAPMVEKWIRAHGG"
     gene            9585..9842
                     /locus_tag="VN034_08160"
     CDS             9585..9842
                     /locus_tag="VN034_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhX family toxin"
                     /protein_id="HWV60610.1"
                     /translation="MNISKAEQRVLHVLAQGGMIRHRRGEDGHIVEALCFTRDGFVLA
                     STGLPLFNRLRRRGFIGSRGGAPYRITQAGLRAVRAQLDNR"
     gene            9960..11759
                     /locus_tag="VN034_08165"
     CDS             9960..11759
                     /locus_tag="VN034_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWV60611.1"
                     /translation="MASQSDLSAANASAEPRRKLGSLRMVWQHASRYPLQLIVAAIAL
                     GIAALATLAIPYQFKEMIDSGFIAGGGNVAPHFRLFYAICVALALATALRFYFVSWLG
                     ERTVADIRQAVQRNLLRLAPGFFEENRPSEIASRMTSDTAIIEQVVGTTVSVALRNFV
                     MGVGGIIYLFSLSPKLTAGILLGIPIIILPIVLLGRRLQKVSRTSQDRVADIGATTAE
                     QMGAMKIVQAFGQEAREADRFTAAVEANFATAKRRIRLRAIITATVIGLLFGAITTLL
                     WYGAEGVAQGTITGGTIAAFVLTGGLVAGAFGALTEVYGDLLRAAGAAERLSELLNAE
                     ATIAPPANPRPFPEPPIGTLEFASVEFHYPTRPDAPALHDFSIAIRPRETVAIVGPSG
                     AGKSTLFQLAERFYDPQAGQILLDGVPLTEADPADIRARIAMVPQETVIFAASARDNL
                     RYGNWDASDDELWAAARAANAEEFLRKLPQGLDTFMGEGGARLSGGQRQRVAIARALL
                     RRAPLLLLDEATSALDAESEKLVQDALETLMADRTTIVIAHRLATVRAADRIIVMDDG
                     RIVEEGKHDELVAADGLYARLARLQFQNNLAAA"
     gene            11775..12317
                     /locus_tag="VN034_08170"
     CDS             11775..12317
                     /locus_tag="VN034_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HWV60612.1"
                     /translation="MTMLYDLTVPAYQNGLKALSGELSKASAWGADNGIGELQFAVAR
                     LAPDMFPLASQVRFTCIQALQPLSRLRGIAIPELIEDATDFAGMQEQIAATLAVLDEV
                     DRAALGDDMERTVSFDLPNGMIFDLSAAEYVRDWAQPQFYFHRVAAYAVLRHMGVPLG
                     KADYVAYMMRHLRPGTAPAA"
     gene            complement(12340..12777)
                     /locus_tag="VN034_08175"
     CDS             complement(12340..12777)
                     /locus_tag="VN034_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWV60613.1"
                     /translation="MRNRRSMFHRSIFRADPNGDPDINTTPLIDVMLVMLVMFIITIP
                     PPTHGVDVTLPTGEVAGKILDQNRVTIDRSDVIRWNDEAIDLAELGVLVKQASERAEP
                     ATILLEPDARARYLRVDEAIGAIRRNGGTKLAFPGIESYAGLI"
     gene            complement(13004..>13391)
                     /locus_tag="VN034_08180"
     CDS             complement(13004..>13391)
                     /locus_tag="VN034_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV60614.1"
                     /translation="PPPPPPPPVVECAPGPYIVYFDWDQSNITPEAASTLDNAISAYN
                     RGCTGTQVMLAGHADRSGSATYNVGLSNRRNDAVRSYLTARGISDGSISAQGFGETRP
                     AVATADGVRNDQNRRVEITYGPNSGM"
BASE COUNT         2384 a         4566 c         4119 g         2322 t
ORIGIN      
        1 cttcgccgcg ggccgcagcg agacctgacc cgccgacgcc gtatcacggg ggaaccatcg
       61 gcggcggcga ggcgctatgg acccatgtcc gcaccgcgtc ttgtccatgt cgacgacagc
      121 ctgcccggca tcagccgcgc gcgctcgggg gtcggctggc gctaccgcga cgccaaggga
      181 aagatcatcc gcgaccgcga cgagatcgac cgcctcaacg cgatcgcgct gccgcccgcc
      241 tatgaggatg cgtggttctg ccccgcagcg aacggccaca tcctcgcgac cggctatgac
      301 gcgcgcgggc gcaagcaata tcgctatcat cccgattttc ggatggcgcg cgaggccgac
      361 aaatatgacc ggtgcgccgc cttcggccac gcgctgccgc ttctccgcgc gcgcctcgaa
      421 gacgatctga accgccggac gctctgctcg gaacgcgtcg tcgccgcggt cgtgcgcctg
      481 ctcgacctcg ccgcgctgcg cgtcgggaac gagcaatatg tcgcggcgaa caagagcttc
      541 ggcgcgacga cgctgcgcca acgccacgcc aagctgtcgg gcaagacgct tcgcctgaaa
      601 tatatcgcca aaggcggggc gaagcgcgag gtgacgatca gcgaccgcag cctcaccacg
      661 ctggtgcgcc ggttgcagga cctgcccggc cagaatctct ttcaatatga ggatgggggc
      721 gacctctgcg tcgtcggctc cgacgatgtg aacgcctata tccgcgaagc gatgggtgac
      781 gaattcagcg ccaagcattt ccgcacctgg tcggcgagcg tcgaggcatt tgccttcctc
      841 tacgacgcgc ccgaaccgcc gaccttgaag gcgatgctcg aacatgtcgc gagccgcctc
      901 ggcaacaccc ccgcgatcgc gcgcaaagcc tatgtccacc ccgcgatgct cgctgcggcg
      961 caggcacgcg aagacttcgc atccgccatc ggaaacctgc cgcgcgcgac gcgttatctt
     1021 tcgcgttacg aacgcggatt tttgacctat ctggagcagg cgcccgcggc tgcggtgctg
     1081 ctccaatccg cctgatccga cgtacaaagg acccgacgtg aatttctcca tcctcgccgc
     1141 cgcgaccacc gccgccccga aagctgcgcc cgcaaaggcg gccgcgaccg atccgctcgc
     1201 cgatctgcag cgctattggg acatgagcct cgcgtgggtg gaaagccact ggctgcagat
     1261 cggtatcgcg atcggcgtcg gtctcgccat ctatttcctg ctgtcgatgg tccgccgctt
     1321 tgcgctcaaa tatgcaaagt cggccgaagg cgacctctcg ctgacccaca tcgccgggcg
     1381 cgtgatccac aagaccaagt catccgtgct cgcgatcatc gcgatccgca tggtcgcgag
     1441 ctatgcccag ccgcccgcgg tgatcatgca tatgatccag ttcgtcttta ccgtcgcggt
     1501 cgtgctgcaa gtcgcgatct gggcgcgcga gatcgtgctc ggactgatcc agcgccgcgc
     1561 cgccgagggc aataacgaga cgctcagcaa cgcgatgggg atcatccgcc tgctgatcag
     1621 tatcgccttg ttcgcgatcg cggcgatcgt catcctcgac aatatggggg tcaatgtcac
     1681 cggcctcatc gcgggcctcg gcatcggcgg catcgcgatc ggtcttgccg cgcaaggcat
     1741 tttctccgac ctcttcgcct cgctgtcgat catcttcgac cgcccattcc gcgtcggcga
     1801 gacgatcaag tacgacatga gcaccgcgac ggtcgaacgc atcgggctca agagcacccg
     1861 gctccgctca ttgaacggcg aattgctcgt gatctcgaac accaatttgc tgagcaagga
     1921 gatcaccaac ttcgcgcatc tccaccgccg ccggatcacc ttcaagatcg gtgtcatcta
     1981 tcagacgacc cccgctatgc tccgcgacct gccgccgttg ctcgaggagc aggtggttgc
     2041 cggtggccag gaattcatcc gctcgagctt tttgaccttc ggtccgtcga gcctcgattt
     2101 cgaactgatc tttgatgtct tcagcgacga ttatgatgtc gtcgccgcgg cgcgcaccga
     2161 tatcgccatc cgcgtcttcg acgcgatggc caagagcggc tacgagttcg cctatcccac
     2221 gcagacgacc tttaccgcgg cgcccgacgg gaccatgatc atgccctatt ccgagtctcc
     2281 aaagccaaag gcggcggcgg caaaggcagc gaagcccggc tgacgctacg gcgccgcggc
     2341 tgcggggctt ggctcttgtg caacgcggca agagatgcct agaacgcgcc ccgaaccagc
     2401 aatttcaggg gatgacatat ggccaccatc acgccgcagg aactgcaaac caaggtcggt
     2461 gagcatctcg gcacgtcgga ctgggtgctc gtcgatcagg agatgatcaa caagttcgcc
     2521 gacgccacgg gcgaccatca gttcatccat atcgacgagg aaaaggccaa gctgacgccg
     2581 ttcggcggca cgatcgcgca cggcttcctc accctgtcgc tgatgccgct gctcggcgcg
     2641 agcaccgacg gccccaaggt cggcggcgtc aagatggggg tcaattacgg ctgcaacaaa
     2701 gtccgcttcc tgtcgcccgt ccgctcgggc aagcgcgtgc gcagccatgt gaagctgctc
     2761 gagctcgagg aaaagcgccc cggccagtgg cagcagacca acgaagtcac cgtcgagatc
     2821 gagggcgagg aaaagcccgc cctgatcgcc gaatggatca gccagttttt cgtttgagca
     2881 tagacctccc cggcaaagat tctgccaaac tcttcgaaga ggaattacat catgcgtgac
     2941 gccgtcatcg tttccaccgc ccgcaccccg ctgacaaagg cggcgcgcgg atcgttcaac
     3001 aacacccttg ccccgacgct cggtgcccat gccgtccgcg ccgcggtcga gcgcgccggc
     3061 atcgaaggcg gcgagatcga cgacgtcgtc ttcggcgccg cgatgcagca gggttcgcag
     3121 acgatgaacg tcgcgcgcct gatcgcgctg cgcgccggcc tgcccgtcac cgtccccggc
     3181 atgtcgatcg accgtcagtg ctcgtcgggc ctgatgacga tcgcgaccgc cgcaaagcag
     3241 atcatcgtcg accgtcagga catcgcggtt gccggcggca tcgagagcat ctcgaaggtc
     3301 agcggcagcg gcaaggtgtt catcgagacc gacgccgagc tgatcgcgat gcacaaggac
     3361 acctatatgc cgatgatcgg caccgccgag gtcgtcgcca agcgctacaa catcagccgc
     3421 gaagcgcagg acgaatattc gttccagtcg cagcagcgca ccgccgccgc gcaggccgcg
     3481 ggcaaatatg acgacgagat cgtcgcctgc aatgcgacca tggcggtggt gaacaaggaa
     3541 acgaaggaag tcagcttcca ggacgtcgtc gcgaacaagg acgagtgcaa ccgtcccgac
     3601 acgacgctcg aagggctgtc gagcctgaag ccggtgatgg gcgaaggctt ttcgatcacc
     3661 gcgggtaacg ccagccagct cgccgacggt gcatcggcgt gtgtcgtgat ggaagccaag
     3721 gtcgccgaaa agaagggtct ccagccgctc ggccgctatg tcggcatggc ggtcgcgggc
     3781 accgagcccg acgaaatggg catcggcccc gtcttcgcga tccccaagct gctcgaacgc
     3841 ttcgagctca agatggacga catcggcctc tgggaactca acgaagcctt tgcggtgcag
     3901 gtgctttact gccgcgacaa gctcggcatc ccgaacgagc tgctcaacgt caacggcggc
     3961 tcgatctcga tcggccaccc gttcggcatg accggcgcgc gctgcgtcgg ccacgcgctg
     4021 atcgaaggca agcgccgcgg cgtcaaatat gccgtcgtga cgatgtgcat cggcggcggc
     4081 cagggcgcag cgggcctgtt cgaggtcttc tgatgcggct tgccgctccg cgtatcgagc
     4141 cggtcgaccc cgaccggctc gatgccgacc agcgcgccgc gctcgaaccc ttccttgctt
     4201 ccgatggggg caaggtcggg ggcggcaaga ttctcaacat cttccgcacc ctcgcccatg
     4261 cgcccaaggc gctgaccgcc ttcctcggct ggggcaatta tatcctctcg aagcgcaatg
     4321 cgctgagccc gcggaatcgc gaactcgtca tcctgcgcac cggcttcaat tgccgctcgg
     4381 gctatgaatg gacgcagcac aagcgcatcg ggctcgactg cgggctcacc gaagacgaaa
     4441 tcgcgcgcat caaggccggg cccgacgcca acggctggaa cgagctcgac cgcgcgatgc
     4501 tccgcgcgac cgacgaactg acgaccagcc atttcgtgac cgatacaagc tgggccgcgc
     4561 tcgcgccgct cggcgacaag gggcgcatgg acctcgtcat gaccgtcggg cagtacacgc
     4621 aggtttcgat gatattgaac agcttcggca tccaggtcga ggacggctgg gacgtcgacc
     4681 ccgacctccg ggtctgatta gaaaaacgga gagagatatg agcaacatcg gcatcatcgc
     4741 gaccctgcgc gtgcagcctg ggaaggaaga ggccttcgaa ggcgtgttcg ccgaactcgc
     4801 cgccgccgtc cgcgcgaacg aagccggcaa cagctattac aagctgttcc gcaccgcggc
     4861 ggcgggcgtc tacaaggtca tggaatgtta tgacgacgaa gccgcggtcg aaacgcatcg
     4921 cgcctcggac cacttccgca cgctcggcgc aaaactcggc ccctgcctcg cgggcgcccc
     4981 cgaaatcgag acgctaagcg cagcctgagt ggggggtatc gaccggaagc agccgtcgcc
     5041 cccgcgaagg cgggggccgc tagcgacctt actcagcggc gcccgcgtaa accgacagcg
     5101 gcccccgcct tcgcgggggc gacgactaag gaaacgtcgg cccccatccc tatgtcgtca
     5161 gccgatctcg gcgatctccc ccgcccggtc gccgatcagg tagcgcggcc cggcgccacg
     5221 ctgcgccgcc ttgtcttcgc ggttgtagag cccgcatttc ttgagcgaca ggcagccgca
     5281 gccgatacaa ttgcccagca gttcgcgcgt tcgttcgagc gccgcgatct gcgcatcgat
     5341 ccgcgtgcgc agcccggtgc tgatccgcgt ccagtcggcg ccattgggcg tccgcccggc
     5401 gggcaagcgg gccagttcgt cgccgatctc ctctaggctc aaccccatct gctgcgcgat
     5461 caggatgaag gagacgcggc ggatatcagc gcgcaggaag cggcgctgcc cgccgccggt
     5521 gcgtaacgcc tcgatcaacc ccttcgcctc gtaaaatcgg atcgccgaca ccgcgactcc
     5581 ggtgcgcgcg gcgagttcgc cgatggcgat caggtcggtc cggtgcatga tccgcgttcc
     5641 tcgcttttcg cttgatctaa accttacttg agctttcata agccgctgcg tcaacccgaa
     5701 tcaggagttc ccccgtgacg caccccttca tcgagcatgt gaatctgacc gtcagcgatc
     5761 ccgaccgcac cgcgggcatc ctgtcggcga tcttcggctg gcacgagcgc tggcgcggac
     5821 ccgcacgcga tggcgggcgc acgatccacc tcggcagcga cgccgcctac atcgcgctct
     5881 acaccggccc cgacggccag catgcggatg cgcgctatcc caagggcgaa ccgctcaacc
     5941 atatcggagt gcaggtcgaa gatctcgaca cgatcgagat gcgggtgaag gctgtcgggc
     6001 tcaccccatt caaccacggc gattacgagc ccggccggcg cttctatttc ttcgatcccg
     6061 acggcatcga atatgaagtc gtcagctatg ccgagccgcg acggcgctga accgggcaac
     6121 aagtgcaccg ctgccgcgtt gatcggtcat cgggggacag aaatggagcc tttcgatgag
     6181 cgacgatatc aaacagcagt tttggaaagc cttggccgac agcccctatg tcatgctcgg
     6241 cgcaaccggc gaacgcgaac atcatatccc gatgaacgcg cagctcgaca aggacgcgca
     6301 cggcgctttc tggttcttca ccgcgaccga caaccgcctg gcggccggcg gacccgcgat
     6361 ggcgcaattc gcggccaagg gtcatgacct gttcgcctgc atttcgggaa cgctggtgca
     6421 cgagaatgat cgcacggtgc tcgacaaatt gtggaacaac ggcatcgcgg cctggtatcc
     6481 gggcggcaag gacgatccca agctcgtcct gctgcgtttc gacctcgacg atgccgaaat
     6541 ctggactgcc gatcccggta tcaagggaat gttcaagctc gcaacgggaa tgacgatgaa
     6601 ggacggtgac ctcggcaagc atgctgaggt ggcgctctag ggctagatca ccggcggccc
     6661 gatccgcccc tcgcgaaatt cgaacccgcc ttcgcggtcc cgcgccagca acgcgggacc
     6721 gtcgaggtcg acccatttcg cgcgctgcgc gagcacgaag gccggcgcga tcgcgaggct
     6781 ggtcgagagc atgcacccgg ccatgatcga cagccccgcc gcatcggcgg cgtcggcaat
     6841 cctcagcgcc tcggtcaggc cgcccgcctt gtcgagcttg atgttcaccc cgtcataaaa
     6901 gggtccgatc cgcgcgacgt cggcggcggt ctggcaactt tcgtcggcga tgaagggtag
     6961 cggcgcatag attggatcga gcagcgcatc ggcgcccacc gggaccggct gctcgatcat
     7021 ttcgactccc atgtcggcga gcgcctcggc ctcgctcaaa atatcgatct cgccccagct
     7081 ttcgttcgca tcgacgatca accgcgcctc gggcgcgcct tggcgtaccc cggcgacgcg
     7141 cagccggtcg tcttcgccgg tgagcttgat cttgagcaat cgatagccgt cggcggccgc
     7201 cgtcgccgcc tgttcggcca tcgcccccgg cgtgcccagc gagatcgtat aggcggtaac
     7261 ccgcgatgtc ggcgccccgt cgcagcccgc aagttgccag agccggagcc cgcgttgccg
     7321 cgcttccaga tcccacagcg cgcaatcgag cgcgttgcgc gccgcaccag ccggcatcaa
     7381 cagctgcacc gctgcccgcg cctcagccgc gtccatctcc gcgatatgcg gcgcggcgag
     7441 cagaatctga tcgcggcaac ccgccgcatc ctctccgaga taatagatgg gggtgccttc
     7501 gccgcgcccg accgcgcctt caccctcgat ttcggcgacg acgacatcga cgtgcgtctt
     7561 ggcgccgcgg ctgatgacga actcgcccgc gacggcccag cgttcgacgc gcgcgctttt
     7621 cagttggata gccatgcagg aacagtaatg agggaagcga tgagcggcaa acgtctttcg
     7681 ggcaaatccc tttccgagcg tatcggcgat ctcggccacc gcctcgcgat cgaggcgcac
     7741 gccgcttggc tcgccgcgcg cgatccgcgg gtgccgatgc tcgcgcgcgc tctcgcggtc
     7801 ggggtcgccg cctatgcgct gtcgccgatc gacctcattc ccgacttcat tccggtgctc
     7861 ggctggctcg acgacctcgt catcgtcccg ctggggctgc tcgtggtccg gcgattgatc
     7921 cccgccccgc tatgggcaga gctccacgcc gccgccgaag cggcaagcga gcgcccgtcg
     7981 agccgcgccg gcatggcgtt catcctgctg ctctgggcag ggctgctcta tatcgtctat
     8041 tgggctgtac ggacttcgcc ctggcattga ccggcgcttc cagtcttcag ccgccgtgcg
     8101 cccgaatcca tttttcgacc atgggcgcga tctcctcgcg ccatttgcgg ccgttgaaga
     8161 tgccgtaatg gccgacgccc ttcgccatca gatatttctt ccggtcggcg ggtagcgcct
     8221 tcgcgagcgt cagcgcggcc ttggtctggc cgatccccga aatatcgtcg cgctcgccct
     8281 cgatcgcgag gatcgcgatg tcctcgatgg caccgacatc gaccgtctgg ccgcgatgcg
     8341 tcatctcgcc ctttggcagc aggtgacgct ggaacacgat gtcgaccgtc tggagataga
     8401 attccgccgt catgtcgcac accgcgcgat actcgtcata gaattccttg gtcttgtcgg
     8461 cgctttcgcc gtcgccgtcg accagatgtt tgaacatctc ccaatggctc atcatatgat
     8521 tgccgaggtt catcgacatg aagccggcga gttgcagaaa gcccggatag acgcggcggc
     8581 ccgcgcccgg ataataggcc ggcacggttg caatcacatt ctcctggaac caagcgtgcg
     8641 gccgcgtcat cgcatggtcg ttcaccgcag tcggcgcctt gcgcgtgtcg atcggtccgc
     8701 ccatcatcgt cagcgtcctg ggcctgcatt tatgcttgtt cgccgccatg atcgccgcgg
     8761 cggctaggct cggcaccgac ggctgacaca ccgccagcac atgcgcgccc ggcccgatat
     8821 gctcgagcca cgaaatcaga tagtcgacat aatcgtcgag atcgaaccgg cccgcctcca
     8881 gcggcacatt gcgggcatcg cgccaatcgg tgatccacac gtcatgaccc ggcagcattc
     8941 gctcgaccgt gccgcgcaga agcgtcgcat aatggcccga catcggcgcg acgatcagca
     9001 gcttgggcgc gtctttcgtt cccttgtgct tgaaatgctt gagctggccg aagggcttgc
     9061 gcgcctcgac cacctcggtc acgcgcaccg tctcgccgtc gacgatggtc tcgtaaagtt
     9121 cgaaccccgg cttgccgcgg ggcgcggccg catgcgcgaa gacttcgagt gccgaggcga
     9181 acatcgggct gccgccgaaa tagccgagcg gattcgccgg atgctgcatc acctgcgcgc
     9241 ccgcagtcgc cagcgcactc gcccccgcaa gccagttctt ctgaatttca aaggcatgat
     9301 acagcatatt cactcgttcc ttgcgtgacc cggggcggtg ctcttgcggc acctgcttgt
     9361 tggttagcag tctagggggt tcggctcatt gtgcaatgca tcaaaatcat tgcgggacag
     9421 ggcgatcgcg aataatcagc gtgacgaggc gcatgacgcg ccgctggagt gaatatgtct
     9481 gagattgcgg gcaaccgcta acatcccggc gaaaacggga tgtgcgcagc gcgcgagcca
     9541 atgggttcgc gcgccgttgt tgcttatcaa tccggacata tcccatgaat atctcgaaag
     9601 cggagcagcg cgtgctccat gtgctggcgc agggcggcat gatccgtcat cggcgcggcg
     9661 aagacggcca tatcgtcgag gctttgtgct tcacccgcga cggcttcgtc ctcgccagca
     9721 ccggcctgcc cttgttcaac cggctgcggc gacgcggttt catcggctcg cgcggcggcg
     9781 ctccgtaccg catcacgcag gcggggttgc gagcggtcag ggcacagctc gacaatcgct
     9841 gaaaaaccgg gagcgcggcg caatcgccgc gctcctttgc gttccgcttc aaagcgcatg
     9901 aagcgccaca tcattctgca tcgttgagca cggcccgcgc cgctgctagg gccggtgcga
     9961 tggccagcca gtccgacctt tccgccgcga acgcttccgc cgaacctcgc cgcaaactcg
    10021 gcagccttcg catggtgtgg caacatgcca gccgctatcc gctgcaactg atcgtcgccg
    10081 cgatcgcgct cggcatcgcg gcgctcgcga cgctcgcgat cccctatcaa ttcaaggaaa
    10141 tgatcgacag cggcttcatc gccggcggcg gcaacgtcgc gccgcatttc cggcttttct
    10201 acgccatctg cgtcgcgctt gccctcgcaa ccgcgctgcg cttctatttc gtgagctggc
    10261 tgggcgagcg caccgtcgcc gacatccgcc aggcggtgca gcgcaacctc ctgcgcctcg
    10321 ctcccggctt tttcgaggaa aaccggccgt ccgaaatcgc ctcgcgcatg acatcggaca
    10381 ccgcgatcat cgaacaggtc gtcgggacga cggtgtcggt cgcgctacgc aattttgtga
    10441 tgggcgtcgg cggcatcatc tatctgttca gcctgtcacc aaagctcacc gcgggcatcc
    10501 tcctcggcat cccgatcatc atcctgccga tcgtgctgct cggccggcgg cttcagaagg
    10561 tatcgcggac cagccaggac cgcgtcgccg acatcggcgc aaccaccgcc gaacagatgg
    10621 gcgcgatgaa gatcgttcag gcgttcggcc aggaagcgcg cgaagccgac cgcttcaccg
    10681 ccgctgtcga ggccaatttc gcgaccgcga aacgccgcat ccgccttcgc gcgatcatta
    10741 ccgcgacggt catcggcctg ttgttcggcg cgatcaccac cttgctctgg tacggcgccg
    10801 agggcgttgc gcagggaacg atcaccggcg gcacgatcgc cgccttcgtg ctcacaggcg
    10861 gacttgtcgc gggcgcattc ggcgcactca ccgaggtcta tggcgacctg cttcgcgccg
    10921 ccggcgccgc cgagcgattg agcgagttac tgaacgccga agcgacgatc gcgccgccag
    10981 cgaacccccg ccccttcccc gaacccccga tcggcacgct cgagttcgcg agcgtcgaat
    11041 tccattatcc gacgcgcccc gacgctcccg cgctccacga tttcagcatc gcgatccgtc
    11101 cgcgtgagac ggtcgcgatc gtcggtccct cgggcgctgg caagtcgacg ctctttcagc
    11161 tcgccgaacg cttctatgat ccgcaggcgg ggcagatcct gctcgacggc gtgccgctga
    11221 ccgaggccga tcccgccgac atccgcgcgc gcatcgcgat ggtgccgcag gaaacggtga
    11281 tcttcgcggc gtcggcgcgc gataacctgc gctacggcaa ctgggacgcc agcgacgacg
    11341 agctctgggc tgccgcgcgc gcggccaatg ccgaagaatt tctccgcaaa ctgccccagg
    11401 ggctcgacac cttcatgggc gaaggtggcg cacgcctctc ggggggccag cgccagcgcg
    11461 tcgcgatcgc gcgtgcgctg ctgcgccgcg cgccgctcct gctgctcgac gaagcaacct
    11521 cggctctcga cgccgaatcc gaaaagctcg tgcaggatgc gctcgaaacg ctgatggccg
    11581 accgcacgac gatcgtgatc gcgcaccggc tcgcgaccgt gcgcgctgcc gaccgcatca
    11641 tcgtgatgga cgacgggcgc atcgtcgaag agggcaagca cgacgagctc gtcgctgccg
    11701 acggtctcta cgcccgcctt gcgcgcctgc agttccaaaa caatctcgcc gcagcctgag
    11761 aagatcgaag gaaaatgacg atgctctacg acctgaccgt accggcctac caaaatggtc
    11821 tgaaggcgct gtccggcgag ttgtcgaagg caagtgcatg gggcgcggat aacggcatcg
    11881 gcgagttgca attcgcggtc gcgcggcttg cgcccgacat gttcccgctc gcctcgcagg
    11941 tccggttcac ctgcatccag gccttgcagc cgctgagccg gctcaggggc atcgctatcc
    12001 ctgaacttat cgaagatgcg accgacttcg ccggaatgca ggaacagatc gccgcgacgc
    12061 tcgccgttct cgatgaggtt gatcgtgccg cgctcggcga tgacatggaa cggacggtca
    12121 gcttcgactt gccgaacggc atgatcttcg acctcagcgc tgcggaatat gtccgcgact
    12181 gggcgcagcc gcaattctac ttccaccgcg tcgcggccta tgcggtgctg cgccacatgg
    12241 gcgtcccgct cggcaaggcc gattatgtcg cctatatgat gcgccacctc cgccccggaa
    12301 ccgcgcccgc cgcctgagcg gcgacggggc gctccgatct cagatcagcc cggcgtaact
    12361 ctcgatacct ggaaaggcga gcttggtccc gccattgcgc cggatcgccc cgatcgcctc
    12421 atcgacgcgc aggtaacgcg cccgcgcatc gggttcgagc aggattgtcg ccggctccgc
    12481 acgctccgac gcctgcttta ccagaacgcc cagctcggcg aggtcgatcg cctcgtcgtt
    12541 ccaccggatc acatcgctcc gatcgatcgt aacgcgattt tgatcgagta tcttcccggc
    12601 gacctcgccg gtcggcaacg tcacgtcgac gccgtgtgtc ggcggcggaa tggtgatgat
    12661 gaacatcacg agcatcacga gcatgacgtc gatcagcggc gtggtgttga tgtcgggatc
    12721 gccattcggg tcggcgcgaa aaatgctgcg atggaacatg ctacggcggt tgcgcatcgt
    12781 ctctctcccg ttcgggaggt gcggccccgc tatacttcgg gcgctcgccc ctcgacaggg
    12841 catgaaacca tctcagcccc tattgtgatg gtatatcatt attccaaacg aacaagtggt
    12901 atcgtgcgaa cgcgccgagc gaggcaaata caaaaagaaa gggcggcacc tttcggtacc
    12961 gcccctcttt tcttccagtc cccgtcgggg atggaaaccg gtcttacatg cccgagttcg
    13021 gaccgtaagt gatttccacg cgacggttct ggtcgttgcg gacgccgtcg gcggtcgcaa
    13081 cggccgggcg ggtttcgccg aagccctgcg cgctgatcga gccatccgag atgccacgag
    13141 cggtcaggta gctgcgaacc gcgtcgttgc ggcggttcga caggccgacg ttgtacgtgg
    13201 ccgaacccga acggtcggcg tgaccggcga gcatgacctg cgtgcccgtg caaccacggt
    13261 tgtaggcgct gatcgcgttg tcgagcgtcg aagccgcttc cggcgtgatg ttcgactgat
    13321 cccagtcgaa atacacgatg tacggcccag gtgcgcattc cacgaccggc ggaggcggcg
    13381 gaggcggcgg g
//
LOCUS       DATMSU010000298        20010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_146997,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000298 DATMSU010000000
VERSION     DATMSU010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 20010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20010
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146997"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VN034_08185"
     CDS             complement(<1..598)
                     /locus_tag="VN034_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008671 - 2-dehydro-3-deoxygalactonokinase
                     activity [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HWV60615.1"
                     /translation="MSTLSAFLAVDWGTTNRRVFRIEHGAVAHTERDDRGAASVTDFA
                     GEVVAIRERFGDLPMLLAGMVGSTVGWRVVPYVAAPAGIDELVAGLDWIDDRTAIVPG
                     VSCSAGGRADVMRGEEVQLLGAVAGWMVPPDALLAQPGTHCKWATMTDGRISGFTTAM
                     TGELFALLRGHSLLAGALTADVTDGDAFREGLAEGARRD"
     gene            complement(595..2229)
                     /locus_tag="VN034_08190"
     CDS             complement(595..2229)
                     /locus_tag="VN034_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HWV60616.1"
                     /translation="MSGLSQIDIIVIVIYAVGIFGLAQWVSREKAGHAKNTSDYFLAS
                     KSLPWWAIGASLIAANISAEQIVGMSGSGYAVGLAIASYEWMAALTLLIVGKYFLPIF
                     LKNEIYTMPQFLEQRFGPTIRTVMAIFWLVLYIFVNLTSILWLGSIAVTQVAGVNQDV
                     ALFGLGAFALVYQLRGGLKAVALTDIVQVTLLVFGGLVISYLTLAQIGGDAGVLGGFT
                     RLTTELPEKFDMILSQDNPFYKDLPGLSVLIGGMWIANLSYWGFNQYIIQRALAAKNL
                     GEAQKGVVFAAFLKLLMPVIIVLPGIAAVILAPDLAKPDQAYPTMMGLLPVGLLGLVF
                     AALVAAIIASTASKINSVATIFTLDLYAKMKGVQSRAQNAGQDTALTGAHERQLVFVG
                     RITAVVATLLAIFTARPLLGSLDQAFQYIQEFSGFITPGITVIFLLGLFWPRATEAGA
                     LVGAVASVLFSLIFWFPAEWGGVSAFNAVPFMDRMLIVFFASLALAVIVSLARPARAD
                     SNRIVMQGVSFATTKGFNVAGVLVILILIALYATWW"
     gene            2357..3097
                     /locus_tag="VN034_08195"
     CDS             2357..3097
                     /locus_tag="VN034_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWV60617.1"
                     /translation="MSGLGIEDTGPQLGRNLTYGMLDAIGRAIVVGDYDGATFPTEAE
                     LAKQHGVSRSVTREAVKMLTAKGLLSARPRQGTTVQPTTSWNLFDTDVLRWLLERQFS
                     IDLLRQFNELRVAIEPEAAALAARVATADDIERVNAGLKRMERAERGDDDALESDIAF
                     HVAVLRASRNPFYAQFRDVVSTALRTSIRFTNRVKGRTANVADHAAVRDAIVAGNSEK
                     ARKAMRALISDVLELIDTANPDGAAPGR"
     gene            complement(3113..4258)
                     /locus_tag="VN034_08200"
     CDS             complement(3113..4258)
                     /locus_tag="VN034_08200"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HWV60618.1"
                     /translation="MTKLAWGAAAAALVAATPALGAEAAQSTFGKMPDGRSVAAVTLT
                     NANGISAKVIALGATLQSVVMPDRDGKKADVALGYDNLSDYIDTPQYFGSTVGRFANR
                     LAGGRFKLDGKTWQTPVNNGANALHGGTLGFDKVLWEVTSVKSGPTASVTLRYVSPDG
                     DQGYPGTMTVDAIYSLDENNDLRIEYVATTDRPTIANITNHAYWNLSGEGSANGAMGH
                     VVMIPAESYLPTDSGAIPTGEYRPVAGTVFDFRKPTVIGERVRDASDEQIVFGRGYDH
                     NWVIGRKVTADQHLMARVEDPASGRGFELWSNQPGLQMYSGNFFDATSHGKSKKIYRM
                     GDALVMEPQIFPDAPNQKNFPNARLAPGETYRNVMTYRLTTGAKTKK"
     gene            complement(4272..5126)
                     /locus_tag="VN034_08205"
     CDS             complement(4272..5126)
                     /locus_tag="VN034_08205"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWV60619.1"
                     /translation="MSDVRVIARARRDRLGEGPMWSARHDALFWVDILGRRINRMALA
                     DGRVDSFEQPHYAAWIIEREVGGFVAGIGRDIVRIDLDADAREPIVAVDPRNAGNRLN
                     DAKADATGRIWAGTIPVTCDRPSGAFYRLDPDGTLAQVDGPYTIANGPAIAADNSFLL
                     HTDTARGTIFRFDIHDDGSLGDAAPFIVFEEAWGNPDGMTFDAEGGLWVACWGASRVM
                     RFAPDGTPDRHIPLPASQITSCTFAGPGLDRMFVTSASDGVTEEHGGALFEVDPGCRG
                     LPTQKYKG"
     gene            complement(5126..6937)
                     /locus_tag="VN034_08210"
     CDS             complement(5126..6937)
                     /locus_tag="VN034_08210"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HWV60620.1"
                     /translation="MGDGAKTRPEGKGGLRSRAWFDNPDNPDMTALYLERYLNYGFSI
                     DELQSGRPIIGIAQTGSDLTPCNRHHLELAKRVRDGIRDAGGIAIEFPTHPIQETGKR
                     PTAGLDRNLQYLSLVEAIYGYPLDGVVLTVGCDKTTPACLMAAATVNIPSIALSVGPM
                     LNGWFKGERTGSGTIVWRAREMLAAGEIDYKGFLELVASSAPSTGWCNTMGTATTMNS
                     LTEALGMSLPGSAAIPAPYRDRQECAYRTGMQIVEMVHADRKPSDILTRTAFLNAIRV
                     NSAIGGSTNAPIHLNAIARHIGVELTLEDWEAQGADVPLIVNLQPAGSYLAEDFYRAG
                     GVPAVMGQLLRAGLIEGAALTANGQTVAANIGEADTQDIDVIRTLDAPLKPAAGLTVL
                     RGNLFDNAVMKLSVISPEFRERYLSNAADPDAFEGVAIVFDGPEDYHGRIDDPALGAD
                     ANSILIMRGAGPIGYPGGAEVVNMRPPAALIQAGVHALPCIGDGRQSGTSGSPSILNA
                     APEAVVGGGLALVRTGDRIRIDLAKRTANMLVDDAELDRRRAELQAEPDYMPPAQTPW
                     QEIHRALTGQFDSGAVLEMAVKYQRVAQAHGLPRDSH"
     gene            7050..7967
                     /locus_tag="VN034_08215"
     CDS             7050..7967
                     /locus_tag="VN034_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWV60621.1"
                     /translation="MIRLGLVGIGKIARDQHLPAVRADDRYELVATASRHAQLDGVPG
                     HADIAAMIAGDHRLNAVSICTPPVGRHAIAAAAIDAGLHVMLEKPPAATTTEISALAD
                     HARVRGVTLFATWHSREAAGVAAAREWLRDKRIRAAHIRWKEDIRRWHPGQEWILDAG
                     GFGVFDPGINALSIMTAILPAPLLLDAARIDIPDGRSSPIAATMTMRSGDAAVTADLD
                     FLQTGPQSWDIEVETDAGTLRLSMGGSVLQLPGGEPVTREDREYPRLYTRFAELVAAG
                     KSDVDIRPLQLVADAFLVAERRSVAPFQF"
     gene            complement(8067..9974)
                     /locus_tag="VN034_08220"
     CDS             complement(8067..9974)
                     /locus_tag="VN034_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HWV60622.1"
                     /translation="MTVQMKSWITGLSLAALSLAPSADAQQKLKAAVADDIFADHPSM
                     DKNATVMPAADAKAVAPIFSERPIGDASIARLPGGGWILTGTSLRSGARHGVELWTSP
                     DAKKWTRIGPAKISGEGIAPGDVSERYLAPSVTVAGDRLYLAFSDKTGCARVATGRAA
                     QPGGTFKASPCLVEDASDVSLFVDEDGTGYLLWGGGYIAKLAPGFGKLAEAPRFLKPD
                     QALFAKHPPVGKDWPVRTRVGTRGATMVRDGDRYVLAASEVTGRMRTATEDLFVAEAP
                     TPYGPFSMRALAVPHGGRGSIVRLDDGRLAAVYNPKCDDGFAMFCEQVGLVPLERSPD
                     GRLRQAASVITEDSAVAARKALVTSETMRDPSVTLGGDGFYYLVGTLDGYGYHKPDGG
                     VKLWRSSDLKQWDEVGFIWKWEDMGYDFGKNIAELWAPEIMWSARDKTYYLAFSVMEK
                     GVGGKTWLYRSKTGKADGPYENVTKSFFVKGIDGFPFEDNDGLYFLWGGGNIAKLNAA
                     RSGFEGPVRQLVDADGDHVGYEGNGLIKANGVYFLTGAEWHGPLRTHGTYDMMYGTSK
                     SLFGPYTQRRMGAPHGGHGTAFRDKEGRYWYTMFGNDPTAPWRMQFGLVPIDIGDAES
                     IGIPRIDAARD"
     gene            complement(9971..11092)
                     /locus_tag="VN034_08225"
     CDS             complement(9971..11092)
                     /locus_tag="VN034_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60623.1"
                     /translation="MRGLWTALAALACLPGVAQAQGALGANFNEHYEDVDYRDLEKAG
                     ARWVRLFVPMPQLDGGAAEHGAVKAILDAHSRGYKTILTLKFPYNRGDFPKTGTPAFE
                     RELARIDAVLPLVVGKVDILVVGNEPYIESREKDRDLDLNAFYEAMAKRVIAYRAKAC
                     PGDCKTRLYMGALNRLDLKKNRTASTERWMAFVKATPELDGVNIHPHIPAIQASKPFL
                     DYILPRMRPEQRFIVTEFSLVWWWKQNMKGVVAPAFADKYAAPRNAQNWQIIKAALET
                     PFPKEQWDDFLRMSPWFENRKHYVANQMKMFRDTGRLAVATYGFKQGRSMSNNFGPDK
                     TPWLINSVFAPATIRRNADGSAAPNYTIFDDFKALQKGK"
     gene            complement(11115..12692)
                     /locus_tag="VN034_08230"
     CDS             complement(11115..12692)
                     /locus_tag="VN034_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWV60624.1"
                     /translation="MKSTLSRRALLLAGAGVAVLPSVALAATPRSKPNIIVIYADDLG
                     YGDLGCYGATALKTPHVDRLAARGTRFVNAHSPSATCTPSRYALLTGDYAWRASGTQI
                     LPGDAPTLIRPGQFTLPLMLKKAGYATGVVGKWHLGLGDAKVDWNGEIAPGPLDIGFD
                     YGYFMPATLDRVPTVLIENRRVVNLDPADPIRVDYKAKVGDEPTGAEHPEQLKFKGDP
                     EHSNTIVNGISRIGYMTGGKAARWKDEELTDQLLGKAIDFVTERKDTPFFLYFAMNEP
                     HVPRAPHPRFVGASGMGPRGDTIVQFDWTVGALMDKLAELGIADDTLVILSSDNGPIL
                     FDGYEDQAVELAGEHRPSGPYRSGKYSIYEGGTRVPMIVSWPGHVREGHVSEALVDHV
                     DMLASLAALVGQALPRDAAVDSFDMLMPLMGRSERGRDHVVEDTKLMVTTGSTVASSG
                     ARILALRAGDWKVIRGSVEPHEFHGNAIGTRPRHQLYNLTRDPGEREDLAAKMPELTA
                     RLAARLDKIERDGRSRP"
     gene            complement(12757..13881)
                     /locus_tag="VN034_08235"
     CDS             complement(12757..13881)
                     /locus_tag="VN034_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60625.1"
                     /translation="MLRKWVGLAGAVLALQSGAAMAQGALGANFNEHYEDVDYRELDS
                     AGAKWIRLFLPMPQVDRGAAEHGAIRTTLEAGARGYKTIFTLKWPQNDRDFPKAGSTE
                     FARELARLDAVLPLVMGKVDILVIGNEPYFETREQDRDLDLNAFYEAMADRIIAYRKA
                     NCGSDCKTKLYMGALNRLDLKKNRTASTERWLAFVKATPEIDGVNIHPHVPKIELSKA
                     FLDYILPRMRPEQTFIVTEFSLVGWWQQNLVKPVAPAFADKYQAPRNAQNWQIIQAAL
                     ETPFPKEQWDDFLRMSPWFENRKHYIANQMKIFRDTGRLAVATYGFKQGSSMSNNFGP
                     KKTPWLLNSVYAGRTIRPNADGTAAFNYAWIDDFRRMQAK"
     gene            13976..15430
                     /locus_tag="VN034_08240"
     CDS             13976..15430
                     /locus_tag="VN034_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWV60626.1"
                     /translation="MKSAKIGRRWAIGAAGAALFAMLGAGILPAHGSTSAPRPEETGP
                     RAPTRQPNILFILIDDMGYADLSLTGNKLVATPNIDALARDGMVMTQFYDAAPICSPS
                     RAGFMTGRFPARDRFVTYIHDRKRNRAFGQADWLDPSLPVLPRMMRQAGYATGHFGKW
                     HLGGGRDIGDAPLPSAYGFDESYTQWEGLGPRVLPTDLTNRLGQQSAELGQGPVEWLP
                     RAEITRRFVDKTLDFVGRSKAKPWFAQLWLTDMHTPWVPNAEQLAATKGKGATPREDQ
                     FLAVLVAMDAEIGRLVDGLRQAGELDDTLIVFTSDNGPTIGVNTPGSAAPYRGRKASL
                     YEGGVRQPLIVRWPGHVASGRRDAQSVAQAVDLLPTLAAITGAPKPAGIDGIDISAAW
                     RGRPLASRPDIYSHIARPGGEAFGPLYSIRSGPWKLLMNADGSSPELYHIERDPQERQ
                     NRMADETAIAKALSAKLAKWIASLPAPATPKRPG"
     gene            15523..18243
                     /locus_tag="VN034_08245"
     CDS             15523..18243
                     /locus_tag="VN034_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60627.1"
                     /translation="MRNRIILACTTSALAIVAMPSTALAQDQDGQVTVDSAPEDPTAD
                     DAIVVTAIQRSLQTSQAIKEDSDQIVDSIVAEDIGKLPDVTATESLARITGVQVDYAN
                     GTAAGTRVRGLPNIATTYNGRELFTGQGRAVALQDFPSSSIARIDVYKSGSANLLEPG
                     VAGLIDVRARKPLDFKGDRIAGGVSGVHWKQSQKLGVDANLLFSKRWETGIGDIGFLI
                     EGSYTDLKFLDSSRNVAQAILVRNNVPGLGTIRYPSFVNINYNTADRYRPSVATALQW
                     RPSNELELYADFLFQGYRSSGYGSNLQINSGVQANLTDIELFPGTNKVKSMTASAGGI
                     PTGNQNIVTGKTDTYQAGTGFIWKRDGLKITGDVAFTDSTYTRQTTQFNYSLVVPQPT
                     RTYDFDTDIGAGGGGVVVSDFPVNDPSRYRMVGLGENGEQSHGRDWQGRLDLDYRMDS
                     TGITNLQAGVRFNSRDFDFANYSESGNAPAGQFYSLLPLDYQTVAPGFRGDKVPITRV
                     FLTPTTDSIMGNLDLLRDLTGDRTEVPPFDRVYYGNEKGYAGYVQGRYAFDLGSMTVD
                     GLVGLRAARTETQINGFNRVTTGGVTTVTPVSQSKSYTDFLPNVSARLRLTPKLQLRL
                     AFTETRTRPGFGDLNPSLTIGAPSAVCNTDPNDPECVRNASGGNADLDPIKSQNYDAS
                     LEWYFSRGGSLTLGLFQRDVTGFISDFTTDVEDAEFGRLRVTRPFNGGEGRLRGIEAA
                     FRTFLRFPQLPEWLQNFGVLANYTYIDHGTELPEALAETLPGKQRIAGVSNHIANASV
                     FYENQSFSARLSYNYRSDFVVVYNRVNDPSLGAAVLGPTLPVVEDGRGSLDFNATLDP
                     TKNVTIAFSATNLLGAAATNHRQYDADGNTYPWQTRFLETIYRVGVRFRF"
     gene            complement(18331..>20010)
                     /locus_tag="VN034_08250"
     CDS             complement(18331..>20010)
                     /locus_tag="VN034_08250"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWV60628.1"
                     /translation="ALPATAQTPAPAAPAAAEQAKRPNVIVILVDDMGFSDIGPYGSE
                     IPTPNLDALAANGLRFTQFYNSARCSPSRASLLTGLYPHEAGMGGLDSIVRPGLGGFQ
                     GRIADRAVTIAEVLKPAGYFTGMAGKWHVGAQHGTPPKAVGFDRSIFHKGGTYFPDQP
                     GKGRTFVTIDGRQVRLDSPEVGKGEWYASDMLVDWTVRFVEEAKAAGKPFFLYLPLTA
                     VHFPVMAPADEIAKFKGKYMAGWDQLRRDRFERQKKMGLIAADATLSEVLPEAYDWDK
                     VPAADKERFDTIMAVYAAAVSRMDKAIGTLVADLKARGEFDNTLILFMSDNGGNAESG
                     PDGRTGAAPWGGKYSNVFVGLNWATLQNTPFRYFKHYAEEGGIATPLIASWPAGIDAK
                     VRGTMVRAPGHLVDVMPTLVELAGAKYPARFDGHDIRPMSGRSFVGAFEGEGLTRSVP
                     IFWEHEGNKAVRDGKWKLVAQFGAPWQLYDMSIDRTETRDLAAAHPDIAAGMAKQWDD
                     WAATSFVDPWPSKIDGGVVKGRLEGGKAPGPGKGKRGKGKRKRAARVAAGE"
BASE COUNT         3628 a         6679 c         6179 g         3524 t
ORIGIN      
        1 ggtcgcgccg cgcgccttcg gccagccctt cgcggaaggc atcgccgtcg gtcacgtcgg
       61 cggtcagcgc ccccgcgagc aggctgtgtc cgcgcagcag cgcgaacagt tcgcccgtca
      121 tcgcggtcgt aaaaccgcta atccggccat ccgtcatcgt tgcccacttg caatgcgttc
      181 cgggctgcgc gagcagcgcg tcgggcggaa ccatccagcc cgcgaccgcg ccgagcaact
      241 gcacttcctc gccgcgcatc acatccgcgc ggccacctgc ggagcaggat acgccgggca
      301 cgatcgcggt gcggtcatcg atccagtcga gcccggccac cagttcgtcg atccccgccg
      361 gcgccgcgac atagggcacg acgcgccagc cgacggtcga gccgaccatg cccgcgagca
      421 gcatcggcag gtcgccgaag cgttcgcgga tcgccacgac ctcgcccgcg aagtcggtga
      481 ccgacgcggc gccgcggtcg tcacgctcgg tatgcgcgac cgcgccatgc tcgatgcgga
      541 acacgcgccg gttggtcgtg ccccagtcga cggcgagaaa ggcggacagc gtgctcacca
      601 ccaggtcgcg tagagcgcga tgaggatcag gatgaccagc acgcccgcga cattgaagcc
      661 cttggtcgta gcgaaggaga cgccttgcat gacgatccgg ttgctgtcgg cgcgcgcagg
      721 gcgtgcgagc gaaacgatta ccgccaacgc aaggctagcg aaaaagacga tgagcatgcg
      781 atccatgaaa ggcacggcgt tgaacgcaga aacccctccc cattcggcag gaaaccaaaa
      841 gatgaggctg aacagaaccg aggcgacggc acccactaac gcccccgctt cggtcgcgcg
      901 gggccaaaac agaccgagca ggaatatgac ggtgatgccc ggcgttatga aacctgagaa
      961 ttcctgaata tactgaaacg cttggtcgag gctgccgagc aacgggcgcg cagtaaagat
     1021 cgcaagaagc gtcgcaacaa cggcggtgat ccggcccaca aaaaccagct ggcgttcgtg
     1081 cgcgccagtg agggccgtgt cctgcccggc attttgcgcc cgcgattgca cccctttcat
     1141 cttagcataa aggtccagcg tgaagatggt ggcgaccgag ttgatcttgg acgcggtcga
     1201 cgcgatgatc gccgccacca gtgccgcgaa gacgaggccg agcagaccga ccggcagcag
     1261 ccccatcatc gttggataag cttgatcggg ctttgccaga tcgggggcga ggatcaccgc
     1321 ggcgataccg ggcagcacga tgatgaccgg catcaacagc ttgaggaatg cggcgaacac
     1381 gacccccttt tgcgcttcgc caaggttctt ggcagccagc gcgcgctgga taatatactg
     1441 gttgaatccc caatagctga gatttgcgat ccacatgccg ccgatcagaa ccgaaagccc
     1501 cggcaaatcc ttgtaaaagg gattgtcctg cgagagaatc atgtcgaact tctcgggtaa
     1561 ttcagtggtc aagcgggtaa agccgccaag cacgcctgca tcgccgccga tctgggcgag
     1621 cgtgagatac gagatgacaa gacccccgaa gacgagcagc gtcacctgga cgatatcggt
     1681 cagcgccact gccttaagtc cgccgcgaag ctgatagacc agcgcgaagg cacctaagcc
     1741 gaacaaggcg acgtcctgat taactcccgc gacctgagtt accgcgatcg aaccgagcca
     1801 caggatcgac gtaagattca cgaagatata gagaactagc cagaatatgg ccatcacggt
     1861 gcggatcgtg ggcccgaacc gctgttccag gaactgcggc atggtgtaga tttcgttctt
     1921 cagaaagatc ggaaggaaat attttcctac gatcagcagc gtcagcgcag ccatccattc
     1981 gtaagaggcg atagcaagcc cgacggcata gcccgatccc gacatgccga cgatctgttc
     2041 ggccgagatg ttcgccgcga tcagcgacgc gccgatcgcc caccaaggga gcgatttcga
     2101 cgcaaggaaa tagtccgacg tgttcttcgc atggcccgcc ttttcgcggc tgacccattg
     2161 cgcgaggccg aaaatgccga cggcatatat gacgatgacg atgatatcga tttgcgacag
     2221 gcccgacata cggactctcc ccttggctga ttattgaaat cacatttatc tgatttaaac
     2281 ggcttgtcta gcttcctgtg cggaatcggt gataaggccg gtcaaaatat agtgtgtgga
     2341 gacgaacgtg gcgaacatgt cgggactggg aattgaggat acggggccgc agctggggcg
     2401 caatctgact tatggcatgc tcgacgcgat cgggcgtgcg atcgtcgtcg gcgattatga
     2461 cggcgcgacc tttccgaccg aggccgaact cgccaaacag catggcgtca gccgatcggt
     2521 gacgcgcgaa gcggtgaaga tgctcaccgc caaagggctg ctcagcgccc gcccccgcca
     2581 agggacgacc gtccagccga ccacctcctg gaacctcttc gataccgacg tccttcgctg
     2641 gctgctcgaa cggcaattct cgatcgacct gctgcgccag ttcaacgagc tgcgcgtcgc
     2701 gattgagccc gaagcagcgg cgctcgcggc gcgcgtcgcg acggcggacg atatcgagcg
     2761 ggtgaatgcg gggctcaagc ggatggaaag ggccgagcgc ggcgacgacg acgcactgga
     2821 aagcgatatc gcctttcacg tcgccgtgct gcgcgcgtcg cggaatccgt tttacgctca
     2881 gttccgcgac gtcgtcagca ccgcgcttcg gacctcgatc cgtttcacca accgcgtcaa
     2941 gggccgcacc gccaatgtcg ccgaccatgc ggcggtgcgc gacgcaatcg tcgccggcaa
     3001 ttccgaaaag gcgcgcaagg cgatgcgcgc gttgatcagc gacgtgctcg aactgatcga
     3061 cacggccaat cccgacggcg ccgcccccgg gcggtgacgg caccggcggc gcctatttct
     3121 tcgtcttcgc tcccgtcgtg aggcgatagg tcatgacgtt gcgataggtt tcgccgggtg
     3181 cgaggcgcgc gttggggaaa ttcttctggt tcggggcgtc gggaaaaatc tgcggttcca
     3241 tgaccagcgc atcgcccatg cgatagattt tcttgctctt gccgtggctc gtcgcgtcaa
     3301 agaaattgcc cgaatacatc tggaggcccg gctggttcga ccagagttcg aacccgcgcc
     3361 ccgacgccgg gtcttcgacc cgcgccatca gatgctggtc ggcggtgacc tttcgcccga
     3421 tcacccaatt atggtcgtag ccacggccga agacgatctg ctcgtcgctg gcgtcgcgga
     3481 cccgctcccc aatgacggtc ggcttgcgga aatcgaacac cgtgccggcc accgggcgat
     3541 attcgccggt cgggatcgcg cccgaatcgg tcggcagata gctttccgcc gggatcatca
     3601 cgacatgccc catcgcaccg ttcgccgagc cttcgcccga cagattccaa taggcatggt
     3661 tggtgatgtt cgcgatcgtg ggccggtcgg tggtcgcgac atattcgatc ctaaggtcat
     3721 tattctcgtc gagcgaataa atcgcatcga ccgtcatcgt gccggggtag ccctgatcgc
     3781 cgtcggggct gacatagcgc agcgtgaccg acgccgtcgg cccgctcttg accgaggtca
     3841 cttcccacaa gaccttgtcg aagccgagcg tgccgccgtg cagcgcgttc gcgccattgt
     3901 tgaccggcgt ctgccacgtc ttgccgtcga gcttgaagcg cccgcccgcg agccggttgg
     3961 cgaagcggcc gacggtcgag ccgaaatatt gcggcgtgtc gatataatcg gagagattgt
     4021 cgtaaccgag cgcgacatcg gccttcttgc cgtcgcgatc gggcatcacc accgattgca
     4081 gcgtcgcgcc gagcgcaatg acctttgccg aaatgccgtt ggcgttggtc agcgtcaccg
     4141 cggccacgct gcgcccgtcg ggcatcttgc cgaaggtcga ttgcgctgcc tcggcaccca
     4201 gcgccggcgt tgccgcaacc aaggccgccg cggcggctcc ccaagccagt ttcgtcatct
     4261 tcttcactcc tctaaccctt gtatttctgc gtcggcaggc cgcggcaccc cggatcgacc
     4321 tcgaacagcg cgccgccatg ctcctcggtc acgccatcgg atgccgaggt gacgaacatc
     4381 cggtcgaggc cgggtccggc gaaagtgcag ctggtgattt gcgatgccgg aagcgggata
     4441 tgccgatcgg gggtgccgtc gggcgcgaag cgcatgacgc gcgaggcgcc ccagcacgcg
     4501 acccacaagc cgccttcggc gtcgaaagtc atcccgtcgg gattgcccca agcctcctcg
     4561 aacacaatga acggcgccgc atcgccgagc gagccatcgt cgtggatatc gaaacgaaag
     4621 atcgtcccgc gcgcggtgtc ggtatgaaga aggaagctgt tgtcggcggc gatcgccgga
     4681 ccattggcga tcgtataggg gccatcgacc tgcgcgagcg tgccgtcggg atcgaggcgg
     4741 taaaaggccc ccgagggacg gtcgcaggta acgggtatcg tccccgccca gatccggccc
     4801 gtcgcgtcgg ccttggcatc gttcagccgg tttcccgcat tgcggggatc gaccgcaacg
     4861 atcggttcgc gcgcgtcggc atcgaggtcg atccgaacga tatcgcggcc gatccccgcc
     4921 acgaaaccgc cgacctcgcg ttcgatgatc caggccgcat aatggggctg ctcaaagctg
     4981 tcgacgcggc cgtcggcgag cgccatgcgg ttgatccgcc ggcccaatat atcgacccag
     5041 aacagcgcgt cgtgccgcgc cgaccacatc ggcccttcgc ccagccggtc gcgacgcgcg
     5101 cgcgcgatca cccgcacatc ggacatcagt ggctgtctcg cggcaagccg tgcgcctgcg
     5161 cgacgcgctg atatttgacc gccatttcga ggaccgcccc gctgtcgaac tggccggtca
     5221 gcgcgcggtg gatttcctgc cacggcgtct gcgcgggcgg catataatcg ggctcggcct
     5281 ggagctcggc gcggcgccga tcgagttcgg cgtcgtcgac gagcatgttc gcggtgcgtt
     5341 tggccaaatc gatacggatg cggtcgcccg tccgcaccaa cgccagcccc ccgccgacga
     5401 cggcttcggg cgcggcgttc aggatcgacg ggctgccgct cgttcccgac tggcgcccgt
     5461 cgccgatgca tggcagcgcg tggacacccg cctggatcag cgcggcgggt gggcgcatgt
     5521 tcacgacctc ggcaccgccg ggatagccga tcggccccgc gccgcgcatg atcaggatcg
     5581 agttggcatc ggcgccgagc gccggatcgt cgatacggcc gtgataatcc tcgggcccgt
     5641 cgaacacgat cgcgacgccc tcgaacgcgt cggggtcggc cgcattgctc agataacgct
     5701 cgcgaaactc gggcgaaatg acgctgagtt tcatcaccgc attgtcgaac agattgccgc
     5761 gcagcacggt cagccccgcg gcgggtttca gcggcgcgtc gagcgtgcgg atgacgtcga
     5821 tgtcctgcgt gtccgcctcg ccgatgttcg ccgcgaccgt ctggccgttg gcggtcagcg
     5881 ccgcgccctc gatcagcccg gcgcgcagca actgccccat caccgcgggc acgccgccgg
     5941 cgcggtagaa atcctcggcc agatagctgc ccgccggctg cagattgacg atcagcggca
     6001 catcggcgcc ctgcgcctcc cagtcctcca gcgtcaactc gacaccgata tgccgcgcga
     6061 tggcgttgag gtggatcggt gcgttggtcg acccgccgat cgccgaattg acgcggatcg
     6121 cgttcaaaaa ggcggtgcgc gtcaatatgt cgctcggttt gcggtcggcg tggaccattt
     6181 cgacgatctg catcccggtg cgataggcgc attcctgccg gtcgcgatag ggcgcgggga
     6241 tcgccgccga cccgggcagc gacatgccga gcgcctcggt cagcgaattc atcgtcgtcg
     6301 cggtgcccat cgtgttgcac cagccggtcg acggcgccga cgaggcgacg agttcaagaa
     6361 agcccttata atcgatctcg cccgccgcga gcatttcgcg cgcgcgccag acgatcgttc
     6421 ccgacccggt gcgctcaccc ttgaaccagc cgttgagcat cgggccgacc gacagcgcga
     6481 tcgacgggat attcaccgtc gccgccgcca tcaagcacgc cggcgtcgtc ttgtcgcagc
     6541 cgaccgtcag cacgacaccg tcgagcggat agccataaat cgcctcgaca aggctgagat
     6601 attggaggtt gcggtcgagc cccgcggtcg gccgcttgcc ggtttcctga atcggatggg
     6661 tcggaaattc gatcgcgatg ccgcccgcgt cgcggatccc gtcgcgcacg cgtttcgcga
     6721 gttcgagatg gtggcggttg cagggggtca ggtcgctgcc cgtctgggcg atgccgatga
     6781 tcggccggcc cgattgcagt tcgtcgatgc tgaaaccata gttcagataa cgttcgagat
     6841 agagcgccgt catgtccggg ttatccgggt tatcgaacca ggcacgcgac cgcagccctc
     6901 ctttgccttc tggccgcgtt ttcgccccgt ctcccatata tatccttctc gatgcacaat
     6961 ttcaggccaa ggccggaatc ggttgataac ctgaaccacg cccgatagga agcatatata
     7021 tcagacaaat aaaaatgggg tgaagaataa tgattcgctt gggattggtc ggaatcggaa
     7081 aaattgcgcg cgaccagcat cttccggccg tccgtgcgga cgatcgatac gagcttgtcg
     7141 ccaccgccag ccgccacgcg cagctcgacg gcgtgccggg acatgccgac atcgccgcga
     7201 tgatcgcggg cgatcaccgc ctcaacgctg tatcgatctg caccccgcca gtcggacgcc
     7261 acgcgatcgc ggcggcggcg atcgacgcgg gactgcatgt catgctcgaa aagccgcccg
     7321 ccgcgaccac gaccgagatc agcgcgctcg ccgatcacgc ccgcgtccgc ggcgtcaccc
     7381 tgttcgcgac ctggcattcg cgcgaagccg ccggggtcgc cgcggcacgc gaatggctgc
     7441 gcgacaagcg gattcgcgcc gcgcatatcc gctggaagga agatatccgc cgctggcatc
     7501 cggggcagga atggattctc gacgccggcg gcttcggcgt cttcgacccc ggcatcaacg
     7561 cgctgtcgat catgaccgcg atcctccccg cccccctgct tctcgacgcg gcgcggatcg
     7621 acatccccga cgggcgcagt tcgccgatcg ccgcgacgat gacgatgcgg agcggcgatg
     7681 ccgcggtgac cgccgacctc gacttcctgc aaaccggccc gcaaagctgg gatatcgagg
     7741 tcgagaccga cgccgggacc ttgcggctgt cgatgggcgg cagcgtgctt caactgccgg
     7801 gcggcgaacc ggtcacgcgc gaagaccgcg aatatccccg cctctacacg cgcttcgccg
     7861 agctggtcgc ggcgggcaag agcgacgtcg atatccgccc gctccagctc gtcgccgacg
     7921 ccttcctcgt ggccgagcgg cgcagcgtag cgccgttcca gttctgacga ctcccgacca
     7981 gtcccgctca cggtgagcaa aaaaagaccc gccaccccgg aaggggtagc gggcattggg
     8041 gagagccgag aaagcggggc gggcgcctag tctctcgcag cgtcgatccg cggaatgccg
     8101 atcgattcgg cgtccccgat atcgatcggc accagcccga actgcatgcg ccacggcgcg
     8161 gtcgggtcat tgccgaacat ggtgtaccaa tagcgcccct ctttatcgcg gaacgccgtc
     8221 ccgtgcccgc cgtgcggcgc gcccatgcgg cgctgcgtat agggaccgaa caggcttttc
     8281 gatgtgccgt acatcatgtc gtaagtgccg tgcgtgcgca gcggaccgtg ccattcggcg
     8341 ccggtcagga aatagacgcc gttcgccttg atcaggccgt tgccctcata acccacatgg
     8401 tcgccgtcgg cgtcgacaag ctggcgcacg ggtccctcga aaccgctgcg cgcggcgttg
     8461 agcttggcga tattgccgcc gccccacagg aaatagagac catcgttatc ctcgaaagga
     8521 aatccgtcga tgcctttgac gaagaagctt ttcgtcacat tctcataggg accatcggcc
     8581 tttccggtct tggagcgata gagccaggtc ttccccccga cccccttctc catcaccgag
     8641 aaagcgagat aataggtctt gtcgcgcgcc gaccacatga tttcaggcgc ccagagttca
     8701 gcgatattct tgccgaaatc atagcccata tcctcccatt tccagataaa gccgacttcg
     8761 tcccattgct tgagatccga cgagcgccac aatttcaccc cgccgtcggg tttgtgatag
     8821 ccatagccgt cgagcgtccc gacgagatag tagaagccgt cgccgccaag cgtcaccgac
     8881 gggtcgcgca tcgtctcgct ggtcaccagc gccttgcgtg ccgcgaccgc gctatcctcg
     8941 gtgatcaccg aagccgcctg ccgcagccgc ccgtcgggcg aacgttcgag cggcacgagc
     9001 ccgacctgct cgcagaacat cgcaaagccg tcatcgcatt tcgggttata gaccgcggcg
     9061 agccttccgt cgtccagccg cacgatcgaa ccgcgcccgc cgtgcggcac cgcgagggcg
     9121 cgcatcgaga acggaccata gggtgtcggc gcctcggcca cgaacagatc ctcggtcgcg
     9181 gtgcgcatcc gtccggtgac ctcgctcgcg gcgaggacat agcggtcgcc gtcgcggacc
     9241 atcgtcgcgc cgcgcgtgcc gacgcgggtg cgcaccggcc agtccttgcc gaccggcgga
     9301 tgcttggcga acagcgcctg atcgggcttc aggaagcgcg gcgcctcggc aagcttgcca
     9361 aaaccgggcg cgagcttcgc gatatagccg ccgccccaga gaagatagcc ggtgccgtcc
     9421 tcgtcgacga acagcgacac gtcggacgcg tcctcgacga ggcagggcga agccttgaac
     9481 gtgccgcccg gctgtgccgc ccgccccgtc gcgacgcgcg cgcagcccgt cttgtcggag
     9541 aaggcgagat aaagcctgtc gcccgcgacc gttaccgacg gggcgagata gcgttcggac
     9601 acgtcacccg gcgcgatgcc ctcgcccgaa atcttggccg ggccgatgcg cgtccatttt
     9661 ttcgcgtcgg gcgaggtcca gagctcgacg ccgtgccgcg ccccgctgcg cagcgacgtg
     9721 ccggtcaata tccatccgcc ccccggcaat cgcgcgatcg acgcgtcgcc gatgggccgt
     9781 tcggagaaga taggcgcgac ggccttcgcg tcggcagcgg gcatgaccgt cgcatttttg
     9841 tccatcgacg gatggtcggc gaaaatatcg tcggcgacgg cggctttcag tttctgctgc
     9901 gcgtcggccg agggcgccag cgacagcgcc gccaacgaca ggccagtgat ccagcttttc
     9961 atctgcacgg tcatttaccc ttctgaagcg ccttgaaatc gtcgaatatc gtatagttgg
    10021 gcgcggcgct gccgtccgca ttcctgcgga tcgtcgccgg cgcgaagacg ctgttgatca
    10081 gccagggcgt cttgtcgggc ccgaaattgt tcgacatcga gcgcccctgc ttgaagccat
    10141 aggtggcgac cgcgagacga ccggtgtcgc ggaacatctt catctggttc gcgacataat
    10201 gtttgcggtt ctcgaaccac gggctcatcc gcaggaaatc gtcccattgt tccttgggga
    10261 agggcgtttc gagcgccgcc ttgatgatct gccagttctg cgcgttacgc ggcgccgcat
    10321 atttatcggc gaacgcgggc gccacgacgc ccttcatatt ctgcttccac caccagacga
    10381 gcgagaattc ggtgacgatg aacctctgct cagggcgcat gcgcggcagg atatagtcga
    10441 ggaagggctt cgacgcctgg atcgccggga tatgcgggtg gatgttgacg ccgtcgagtt
    10501 cgggcgtcgc cttcacgaac gccatccagc gttcggtcga tgctgtgcgg tttttcttga
    10561 gatccaaccg gttgagcgca cccatataga ggcgcgtctt gcagtcaccc ggacacgcct
    10621 tggcgcgata ggcgatcacg cgcttcgcca tcgcttcgta aaaggcgttg aggtcgagat
    10681 cgcgatcctt ctcgcggctc tcgatatagg gttcgttgcc gacgacgagg atgtcgacct
    10741 tgccgacgac aagcggcagc accgcatcga tgcgcgcgag ttcgcgctcg aacgccggcg
    10801 tgcccgtctt cggaaaatcg ccgcgattat atggaaactt cagcgtcagg atcgtcttgt
    10861 aaccgcgcga atgtgcatcg aggatcgcct tgaccgcgcc atgctcggcg gcgccgccgt
    10921 cgagctgcgg catcggcacg aacaggcgga cccagcgcgc accggccttt tcaaggtcgc
    10981 ggtaatcgac gtcctcataa tgctcgttga aattcgcgcc gagcgccccc tgcgcctgcg
    11041 ccacgcccgg caggcaggcg agcgccgcaa gggcggtcca taatccccgc atcgtcatct
    11101 cctcttgatc ctgatcacgg ccggctgcgg ccgtccctct cgattttgtc gagccttgcc
    11161 gcgagccgtg ctgtgagttc gggcatcttc gcggcgagat cctcgcgctc gccgggatcg
    11221 cgcgtcagat tgtagagctg gtgccgcggc cgcgtgccga tcgcattgcc gtggaattca
    11281 tgcggctcca cgctgccgcg gatgaccttc cagtcgccgg cgcgcagcgc gaggatccgc
    11341 gccccgctgc tcgcgacggt cgaccccgtc gtgaccatca gcttggtatc ctcgaccaca
    11401 tggtcgcgcc cgcgctcgct gcgccccatc agcggcatca gcatgtcgaa gctgtcgacc
    11461 gcggcgtcgc gcggcaatgc ctgccctacc agcgcggcga gcgaggcgag catgtcgaca
    11521 tggtcgacca aagcttcgga tacatggccc tcgcgcacat gccccggcca gctgacgatc
    11581 atcggtacgc gcgtgccgcc ttcgtagatg ctgtatttgc cgctgcgata ggggcccgac
    11641 ggccgatgct ctccagcaag ctcaaccgcc tgatcctcat agccgtcgaa caggatcggg
    11701 ccattgtcgc tgctgaggat caccagcgtg tcgtcggcga tgccgagttc ggcgagcttg
    11761 tccatcagcg cgccgacggt ccagtcgaac tggacgatcg tatcgccgcg cggccccatg
    11821 cccgatgccc cgacgaagcg gggatgcggc gcgcgcggga catgcggctc gttcattgcg
    11881 aaatagagga agaagggcgt gtccttccgc tcggtcacga aatcgatcgc cttgccgagc
    11941 agctggtcgg tcagttcctc gtccttccag cgcgccgcct tgccaccggt catataaccg
    12001 atgcggctga tgccgttgac gatcgtgttc gaatgttcgg gatcgccctt gaacttcagt
    12061 tgttcgggat gttcggcgcc ggtcggctcg tcgccgactt ttgccttgta atcgacgcgg
    12121 atcgggtcgg cgggatcgag gttcacgacg cgccggttct cgatcagcac cgtcggcacg
    12181 cggtcgagcg tcgcgggcat gaaatagcca tagtcgaagc cgatatcgag cggcccgggc
    12241 gcgatttcgc cgttccagtc gaccttggcg tcgccgaggc ccagatgcca tttgccgacg
    12301 acgccggtgg catagcccgc cttcttcagc atgagcggca aggtgaattg ccccggccgg
    12361 atcagcgtgg gggcgtctcc cggcagaatc tgcgttcccg acgcgcgcca ggcataatcg
    12421 ccggtcagca aggcataacg cgacggcgtg caggtcgccg agggcgaatg cgcattgacg
    12481 aagcgcgtcc cgcgtgccgc gaggcgatcg acatggggcg tcttgagcgc cgtcgcgcca
    12541 taacagccga ggtcgccata gccgagatca tcggcataga tgacgatgat attgggcttg
    12601 ctgcgcggcg tcgccgccaa ggcaacgctc ggcagcacgg ccactcccgc gccggccagc
    12661 agcagcgcgc gacgcgacaa tgtggatttc aagaccctct cctatccgct tgagtaggat
    12721 atatctgatt tattcaaaat gcgcaacgcg gccccgctat ttcgcctgca tccgtcggaa
    12781 atcgtcgatc cacgcataat tgaacgccgc ggtgccatcg gcattcggcc ggatcgtgcg
    12841 ccctgcatag acgctgttga gcagccaggg cgtcttcttg gggccgaaat tgttcgacat
    12901 cgaactgccc tgcttgaaac cataggtcgc gaccgcgagc cgcccggtgt cgcggaaaat
    12961 cttcatctgg ttcgcgatat aatgtttgcg gttctcgaac cacgggctca tccgcaggaa
    13021 atcgtcccat tgctccttgg gaaaaggcgt ctcgagcgcg gcctggatga tctgccagtt
    13081 ctgcgcgttg cgcggcgcct gatatttgtc agcgaaggcg ggagcgaccg gcttgaccag
    13141 attttgctgc caccagccga cgagcgaaaa ttcggtgacg atgaacgtct gctcgggccg
    13201 catgcgcggc aggatatagt cgaggaacgc tttcgacagt tcgatcttcg gcacatgcgg
    13261 gtggatattg acgccgtcga tctcgggcgt cgccttcacg aacgccagcc agcgctcggt
    13321 cgatgccgtc cggttcttct tgagatcgag ccggttgagc gcgcccatat agagcttcgt
    13381 cttgcaatcg ctgccgcaat tggccttgcg ataggcgatg atgcggtccg ccatcgcttc
    13441 gtaaaaggca ttgaggtcga ggtcgcggtc ctgctcgcgc gtttcgaaat agggctcgtt
    13501 gccgatcacc aatatatcga ccttgcccat caccagcggc aagacggcat cgagccgcgc
    13561 gagttcgcgc gcgaactccg tgcttcccgc cttggggaaa tcacggtcgt tttgcggcca
    13621 tttcaatgtg aagatcgtct tgtagccacg cgcgccggcc tccagcgtgg tgcggatcgc
    13681 gccatgttcg gccgccccgc gatcgacctg cggcatcggc agaaacaggc gaatccattt
    13741 cgcgcccgcg ctatcgagtt cgcgataatc gacatcttca taatgctcgt tgaaattggc
    13801 gccgagcgcg ccctgcgcca tggccgcacc cgactgcagc gccagcaccg cacctgcaag
    13861 tccaacccat ttcctcagca tctcgccctc ccgttccgcg gcacaaactt gactcatctg
    13921 aattatacga taatacagat ataacgaagg cgcagacccg ggcaaggagg aatggatgaa
    13981 atcggcgaag atcgggcggc gatgggcgat cggggcggca ggtgccgccc ttttcgcaat
    14041 gctcggtgcc ggcatccttc ccgcccatgg ctccacatcc gccccgagac cggaggaaac
    14101 gggcccgcgc gctccgacgc ggcaaccgaa catcctgttc atcctgatcg acgacatggg
    14161 atatgccgac ctgtcgctga ccggcaacaa gctggtcgcg acgccgaaca tcgacgcgct
    14221 cgcgcgcgat ggcatggtga tgacgcaatt ctacgacgcg gcgccgatct gttcgccgtc
    14281 gcgcgccggc ttcatgacgg gccgcttccc cgcgcgcgac cgcttcgtca cctatatcca
    14341 cgaccgcaag cgcaatcgtg cgttcggtca ggccgactgg ctcgacccgt cgcttccggt
    14401 cctgccgcgc atgatgcggc aggcgggcta tgccaccggc catttcggca aatggcatct
    14461 aggcggcgga cgcgacatcg gcgatgcgcc gctcccgtcg gcctatggct tcgatgaaag
    14521 ctatacccag tgggaagggc tcggcccgcg cgttctgccc accgacctca ccaaccggct
    14581 cgggcagcag agcgccgaac tcggccaagg cccggtcgaa tggctgccgc gcgcggagat
    14641 cacccgccgc ttcgtcgaca agacgctcga tttcgtcgga cgcagtaagg caaaaccctg
    14701 gttcgcgcag ctctggctga ccgatatgca cacgccgtgg gttccgaatg cggaacaact
    14761 cgctgccacc aagggcaaag gcgccacgcc gcgcgaggat cagttcctcg ctgtgctcgt
    14821 cgccatggat gccgagatcg gccgcctcgt cgacgggttg cggcaggcgg gcgaactcga
    14881 cgacacgctc atcgtcttca cctcggacaa cggccccacc atcggcgtca acacgcccgg
    14941 gtcggcggcg ccctatcgcg gccgcaaagc aagcctctac gaaggcggcg tgcgccagcc
    15001 gctgatcgtc cgctggccgg gtcatgtcgc cagtggccgc cgcgacgcgc agagcgtcgc
    15061 gcaagccgtc gacctgctgc cgacgctcgc ggcgatcacc ggcgcgccaa agccggccgg
    15121 gatcgacgga atcgacattt ccgccgcatg gcgcggccgt cctctcgcct cgcgccccga
    15181 catctacagc cacatcgccc gacccggcgg cgaagctttc ggaccgctct attcgatccg
    15241 gtcgggcccg tggaaactgc tgatgaacgc cgacggcagc agccccgaac tctaccatat
    15301 cgagcgcgac ccgcaggagc ggcagaatcg gatggccgac gagaccgcaa tcgccaaggc
    15361 gctgtcggcg aagctcgcga aatggatcgc cagcttgccc gccccggcca cgccgaaacg
    15421 gcctgggtga cggaaatata tcctccgaac tggcatcacc cgacgcactt tattttatca
    15481 tattaattct atatctgacc gattttgaag ggggaaatta tcatgcgaaa ccgaataatc
    15541 ctggcgtgca cgacatcggc gttggcgatc gtcgccatgc cgtcgacggc gctggcgcag
    15601 gatcaggatg gacaggtcac cgtcgactcg gcgcccgagg atccgacggc cgatgacgcg
    15661 atcgtcgtca ccgcgattca gcgcagcctc caaacgtcgc aggcgatcaa ggaggattca
    15721 gaccagatcg tcgattcgat cgtcgccgag gatatcggca agctgcccga cgtgaccgca
    15781 accgagtcac tcgcgcgcat caccggcgtc caggtcgatt atgcgaatgg caccgcggcg
    15841 ggcacgcgcg tgcgcggtct gcccaacatc gccaccacct ataacggccg cgagctcttc
    15901 actggtcagg gccgcgccgt cgcgcttcag gattttccgt cgagcagcat cgcgcgcatc
    15961 gacgtctata aatcgggcag cgccaacctg ctcgaacccg gcgtcgccgg cctgatcgac
    16021 gtccgcgcgc gcaaaccgct cgatttcaag ggcgaccgca tcgccggcgg ggtcagcggc
    16081 gtgcattgga aacaatcgca aaagctgggc gtcgatgcga acctgctgtt cagcaagcgc
    16141 tgggaaaccg gcatcggcga catcggcttc ctgatcgagg gatcctacac cgacctcaaa
    16201 ttcctcgatt cgtcgcgcaa cgtcgcgcag gcgatcctcg ttcgtaacaa cgtgcccggg
    16261 ctcggcacga tccgttatcc ctcgttcgtg aacatcaact ataacaccgc cgaccgctac
    16321 cgaccgtcgg tggcgaccgc gctgcaatgg cgcccgagca acgagctcga actctatgcc
    16381 gacttcctgt ttcagggcta tcggagcagc ggctatggca gcaatctcca aatcaactcg
    16441 ggcgtgcagg ccaatctgac cgacatcgag ctcttccccg gcaccaacaa ggtcaagagc
    16501 atgaccgcaa gcgccggcgg catcccgacg ggcaaccaga acatcgtcac cggcaagacc
    16561 gacacctatc aggcgggcac cggcttcatc tggaagcgcg acgggctgaa aatcaccggc
    16621 gacgtcgcct tcaccgactc cacctacacg cggcaaacga cgcagttcaa ctattcgctc
    16681 gtcgtgccgc aaccgacgcg cacctatgat ttcgacaccg acatcggtgc tggcggcggc
    16741 ggggtcgtgg tgtccgattt cccggtcaac gatccctcgc gctatcgcat ggtggggctc
    16801 ggcgaaaatg gtgagcagag ccatggccgt gattggcagg gacggctcga cctcgattac
    16861 cgcatggatt cgacgggaat caccaatcta caggccggcg tccgcttcaa cagccgcgat
    16921 ttcgacttcg ccaattattc ggaaagcggc aacgcgcccg ccggtcaatt ctattcgctg
    16981 ctgccgctcg actatcaaac cgtcgcgccc ggcttccgcg gcgacaaggt gccgatcacg
    17041 cgcgtcttcc tgaccccgac caccgacagc atcatgggaa atctggactt gctgcgcgac
    17101 ctcaccggcg accgcaccga agtgccgcct ttcgatcggg tctattacgg caacgaaaag
    17161 ggatatgcgg gctatgttca gggccgctac gccttcgacc tcggcagcat gaccgtcgat
    17221 ggcctcgtcg gcctgcgcgc cgcgcgcacg gagacccaaa tcaacgggtt caaccgcgtg
    17281 acaaccggcg gtgtaaccac ggtgacgccc gtgtcccaat cgaagtccta taccgacttc
    17341 ctgcccaacg tcagtgcgcg cctgcgtctg acgcccaagt tacaacttcg cctcgccttc
    17401 accgaaacgc gcacgcgtcc cggtttcggc gatcttaacc cgtcgctgac catcggcgca
    17461 ccctcggcgg tctgcaacac cgacccgaat gatcccgaat gtgtccgcaa tgcttcgggc
    17521 ggcaacgccg acctcgaccc gatcaagtcg cagaactacg acgcgtcgct cgaatggtat
    17581 ttctcgcgcg gcggctcgct cacgctcggc ctgttccagc gcgacgtgac cggcttcatc
    17641 tccgacttca cgaccgacgt cgaggatgcc gaattcggcc gcctgcgcgt tacgcggccg
    17701 ttcaacggcg gcgaaggccg gctgcgcggc atcgaagcgg cgttccgcac cttcctgcgc
    17761 ttcccgcagc tgcccgaatg gctgcagaat ttcggggtgc tcgcgaacta tacctacatc
    17821 gatcacggaa ccgaattgcc cgaagcgctt gccgagaccc tgccgggcaa gcagcggata
    17881 gcgggcgtgt ccaaccatat cgcgaacgca tcggtctttt acgagaacca gtccttctcg
    17941 gcgcggcttt cctataatta ccggtcggac ttcgtggtcg tatataaccg cgtgaacgac
    18001 ccgtcgctcg gcgcggcggt gctcggcccg acgttgccgg tggtcgagga cggccgcggc
    18061 agcctcgact tcaatgccac actcgacccg acgaagaatg tgaccatcgc gttcagcgcg
    18121 acgaacctgc tcggcgcggc ggcgacgaac caccggcaat atgacgccga cggcaacacc
    18181 tatccgtggc agacgcgttt cctcgagacg atttatcgcg tcggcgtccg cttccgcttc
    18241 tgatcccatc ccccacggga aacattggcc cgcaatcgcc accccggcga ttgcgggctt
    18301 ccttttttaa cacgtgtgac gccgtccgta tcattcgccc gccgcaaccc gcgcggcgcg
    18361 cttgcgcttg cccttacccc gcttgccctt gccggggccc ggcgccttgc ccccttccag
    18421 ccgccccttg accacaccgc cgtcgatctt gctgggccac ggatcgacga agctcgtcgc
    18481 cgcccaatcg tcccactgct tcgccatgcc cgcggcgatg tcggggtgcg cggcggcaag
    18541 gtcgcgggtt tcggtccggt cgatgctcat gtcataaagc tgccagggag cgccgaactg
    18601 cgcgaccagc ttccatttgc cgtcgcgcac cgccttgttg ccttcatgct cccagaatat
    18661 cggtacgctg cgcgtcaaac cctcgccttc gaacgcgccg acaaagctgc gacccgacat
    18721 cggacgaatg tcgtgcccgt cgaaccgcgc cggatatttc gcgccggcaa gctccaccag
    18781 cgtcggcatg acgtcgacga gatgaccggg cgcgcgcacc atcgtcccgc ggaccttcgc
    18841 atcgatcccg gcgggccacg aggcgatcag cggggtcgcg atcccgcctt cctcggcata
    18901 atgtttgaaa taacggaacg gcgtattctg gagcgtcgcc cagttgagcc ccacaaagac
    18961 gtttgaatat ttgccgcccc atggcgcggc gccggtgcgg ccgtcgggac cgctctcggc
    19021 attgccgcca ttgtccgaca tgaagaggat cagcgtgttg tcgaactcgc cgcgcgcctt
    19081 gaggtcggcg accagcgtcc cgatcgcctt gtccatccgc gagacggcgg cggcatagac
    19141 cgccatgatc gtatcgaacc gttccttgtc ggcggcgggc accttgtccc agtcataggc
    19201 ctcgggcaat acctcggaca gcgtcgcgtc cgccgcgatc aaccccatct tcttctgacg
    19261 ctcgaaccgg tcgcggcgca gttggtccca gcccgccata tatttgccct tgaacttcgc
    19321 gatctcgtcg gcgggcgcca tcaccggaaa gtggacggcg gtgagcggaa ggtagaggaa
    19381 gaagggcttt cccgccgctt tcgcttcttc gacgaaacgc acggtccaat cgaccagcat
    19441 gtccgacgca taccattcgc ccttgcccac ctcgggcgaa tcgagccgca cctgccggcc
    19501 gtcgatcgtc acgaaggtcc gccccttgcc tggctggtcg gggaaatagg ttccgccttt
    19561 gtggaatatc gaccggtcga agcccaccgc tttcggcggc gtgccatgtt gcgcgccgac
    19621 atgccatttg cccgccattc cggtgaaata gcccgcgggc ttcagcacct cggcgatcgt
    19681 gaccgcgcga tcggcgatgc gaccctggaa tccgccaagg cccgggcgga cgatgctgtc
    19741 gagtccgccc atccccgctt cgtgcggata aagtccggtg agcagcgagg cgcgcgacgg
    19801 gctgcagcgc gctgaattgt agaattgcgt gaagcggagg ccgttcgcgg ccagtgcgtc
    19861 gaggttgggc gtcggaatct cgcttccata agggccgatg tccgaaaatc ccatgtcgtc
    19921 gacgaggatg acgatgacat tggggcgctt cgcctgctcg gccgcagcgg gggcggcggg
    19981 ggccggggtc tgcgcggtcg cgggaagcgc
//
LOCUS       DATMSU010000299         4831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_80939, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000299 DATMSU010000000
VERSION     DATMSU010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80939"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="VN034_08255"
     CDS             complement(<1..113)
                     /locus_tag="VN034_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HWV60629.1"
                     /translation="MAEQPKFDLTGRVALVTGASSGLGAGFAKALAAAGAKV"
     gene            complement(304..2112)
                     /locus_tag="VN034_08260"
     CDS             complement(304..2112)
                     /locus_tag="VN034_08260"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HWV60630.1"
                     /translation="MAAETAHAAEAAAHGFDLLPIVILLGAAVVAVPLFKRLGLGSVL
                     GYLAAGLAIGPFGLALFSDAQTILHVAELGVVMFLFIIGLEMQPSRLWAMRGEIFGLG
                     AAQVGGAIALLICVGLALGYPVAASFVAGTGFVLTSTAIVMQMLDERREMGQPKGRRI
                     IAILLLEDLAIVPLLALTAFLAPGGEAVSMTDRLIAVAIGLGAIVGLVFAGRYLLDPM
                     FRLLGRAKAREVMTAAALLVVLGAALAMQLAGLSMAMGAFLAGVLLSESSFRHQLEAD
                     VEPFRGVLLGLFFLGVGMALDLDIVAANAGLIAISVAAYMLLKMIVIYAAARLFRSGH
                     AEAVERAVLMAQGGEFAFVLYAAAASVGIIDAESNATLTAIIIVSMALTPVMIILHDR
                     FMPRVEDTADDLDVADDLSASILMIGFGRFGQIVSQPLFANGCSISLIDTDTIAIRES
                     MAFGFKVYYGDGTRLDILHAAGAQNARMIIVATDDRETTLRIVELVKAEFPNTPLLVR
                     AFDREHAVELIHAHADYQIRETFESALMMAEEALRRLEVDEEAIGDIMEETRRRDQER
                     FDIDICNGVYASRHLIQGNVPVEDRDRYIVKPGEGR"
     gene            complement(2211..4556)
                     /locus_tag="VN034_08265"
     CDS             complement(2211..4556)
                     /locus_tag="VN034_08265"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HWV60631.1"
                     /translation="MKRLTAAAALLALLPIQSFAEQPAPDPLAFVDPMIGTGPEGHTF
                     PGATAPFGMVQLSPDTDATCQIRDCYDHAAGYSYHDPTIQGFSHTHFSGAGHSDLGDI
                     LLMPQVGEVKLDPGDPKQPGSGYRQRFSHDSEKASPGYYAVTLADSGIRAELTAGTRV
                     GVHRYSFPAGKEAHLLLDLRSSLYDYPGKILWSGLHLRPDGTLTGFRETRGWAPGRKL
                     FFAMRFSAPLKSHAFLDRDEKIPYKGFHPPGRGTDALAEKLGKALEVRLDFGALAGPL
                     EVRVALSGVDEAGAVANLDAAEKKAGGAGFDAVRARTEGEWRKALGALEIEAPAPMRS
                     NLYTALYHSLLAPSVWSDVDGRYRGPDDAVHSAKDFTFRSTFSLWDTFRAQHPLLTLV
                     APDQTNTDIVKSLVASRKASPHGILPVWQFHGRETWTMIGYHAVPVIADAYLKGVGDF
                     DANEALDAMIASAEYAPYGGLGDYMTLGYVAIDREPEAASKTVEYAYDDWTIARMAEK
                     MGRKDIADRFYKRAGYWRNSFDAKTGWLRARKADGSFRTPFDPTAINYGSDYTEGNAW
                     QYSWFVPQDQAALFDILGGDAKTVAKLDAMFDYDISKLDYSHAEDIAGLIGQYIHGNE
                     PSHHVAYLYAYAGAPWRTQERLKQIVDSQYKPTPDGLSGNDDLGQMSAWLVFTSLGFY
                     PVAPGSNQYVIGRPFVDRAVLNLPGGKRFTVETVGLSDANPYVGKVELNGKPLARSWI
                     SDAEIRSGGTLRFTMQAKPDAQWGKAAAARPYSLSTARRAP"
     gene            complement(4613..>4831)
                     /locus_tag="VN034_08270"
     CDS             complement(4613..>4831)
                     /locus_tag="VN034_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HWV60632.1"
                     /translation="CTTGPMRVAMGGGVLAGQPQLLPRINAQLEKSLADYMQIPGNGA
                     YVIAPELGTMAGPLGAIALAMDAVGVAA"
BASE COUNT          839 a         1568 c         1637 g          787 t
ORIGIN      
        1 accttcgccc ccgcggcggc aagcgccttg gcaaagcccg cgcccagccc cgacgacgcg
       61 ccggtgacca gcgcgacgcg ccccgtgaga tcgaactttg gttgctccgc catcattttc
      121 ccttccttcg cttgctacgc attggtcgcg attgacgtta acgtcaacca aacaagaccg
      181 gagaaaaagc gcggcaaagg ggttgacgga cttatcttgt tctcctaatg ttcctcttgt
      241 gcacaacgaa cctcatcccc cgtcgcggct gccgagcgta gcgggcgggg gataaaggtc
      301 cggtcagcgt ccttcgccgg gcttgacgat atatcgatcg cgatcctcga ccggaacatt
      361 gccctggatc agatgtcggc tggcgtagac gccgttgcag atatcgatgt cgaaccgttc
      421 ctgatcgcgc cgccgcgttt cctccatgat gtcgccgatc gcttcttcgt cgacctcgag
      481 ccgccgcagc gcctcttccg ccatcatcag cgccgattcg aaggtctcgc gtatctgata
      541 gtcggcatgg gcgtggatca gctcgaccgc atgttcgcgg tcgaaggcgc ggacgagcaa
      601 cggcgtgttc ggaaactccg ccttcaccag ttcgacgatc cggagcgtcg tttcgcgatc
      661 gtcggtcgcg acgatgatca tccgcgcatt ctgcgcgccc gcggcgtgaa ggatgtcgag
      721 ccgcgtcccg tccccgtaat agaccttgaa accgaaggcc atcgactcgc ggatcgcgat
      781 cgtatcggtg tcgatcagcg agatcgagca gccgttggcg aagagcggct ggctgacgat
      841 ctggccgaaa cggccgaaac cgatcatcag gatgctggcg ctgagatcgt cggcgacatc
      901 gaggtcgtcg gccgtgtcct ccacgcgcgg catgaaccgg tcgtgcagga tgatcatgac
      961 cggggtcagc gccatcgaca cgatgatgat cgcggtcagc gtcgcgttgc tttccgcatc
     1021 gatgattccg acgctcgccg ccgcggcata gaggacgaag gcaaattcgc cgccctgcgc
     1081 catcagcacc gcgcgctcga ccgcctcggc atggccgctc ctgaacagcc gcgcggcggc
     1141 atagatgacg atcatcttga gcagcatata ggcggcgacc gagatcgcga tcagcccggc
     1201 attggcggcg acgatgtcga gatcgagcgc catgccgacg ccgagaaaga agaggccgag
     1261 caagacgccg cggaacggct cgacatcggc ttcgagctgg tgacggaagg aggattcgga
     1321 caggagcacc ccggcgagaa aggcccccat cgccatcgac agccccgcga gctgcatcgc
     1381 cagcgcggcg ccgaggacga cgagcagcgc cgcggcggtc attacctcgc gcgccttcgc
     1441 gcgcccgagc aggcggaaca tcgggtcgag cagatagcgg cccgcgaaga ccaggccgac
     1501 gatcgcgccg aggccgatcg cgacggcgat cagccggtcg gtcatcgaca ccgcctcgcc
     1561 gcccggcgcg aggaaggcgg tgagcgcgag caagggcacg atcgccagat cctcgagcag
     1621 caggatcgcg atgatccgcc gccccttggg ctgacccatc tcgcgccgtt cgtcgagcat
     1681 ctgcatgacg atcgcggtcg aggtcagcac gaagccggtg cccgcgacga agctcgccgc
     1741 caccggatag ccgagcgcga gcccgacgca gatcagcagc gcgatcgccc cgccgacctg
     1801 cgccgcgccg agcccgaaaa tctcgccgcg catcgcccat agccgcgacg gctgcatctc
     1861 cagcccgatg atgaagagga acatgacgac gccgagttcg gcgacgtgga ggatcgtctg
     1921 cgcgtcggag aagagcgcga ggccgaacgg accgatcgcg agccccgccg cgagataacc
     1981 gagcaccgag ccgaggccga gccgcttgaa caagggcacc gcgacgacgg cggcgccgag
     2041 caggatgacg atcggcaaca ggtcgaaccc gtgcgcggcc gcctcggcgg catgtgctgt
     2101 ttcggcggcc atcgcccgtc cccttgtccg atctcaaccc cggaacccgc acggaaagac
     2161 aggttcgaat cgaggcttag agcaaggggc gccggcccgc cagcgccggg tcagggcgcg
     2221 cggcgggcgg tcgagagcga atagggacgc gccgccgcag ccttgcccca ttgggcatcg
     2281 ggcttcgcct gcatcgtgaa gcggagcgta ccgccgctgc ggatttcggc gtccgaaatc
     2341 cagctgcgcg cgagcggctt gccgttgagt tcgaccttgc caacataggg gttcgcgtcg
     2401 gacagcccga ccgtctcgac cgtgaagcgc ttgccgccag gcaggttcag caccgcgcga
     2461 tcgacgaagg ggcgtccgat cacatattgg ttcgatcccg gcgcgacggg gtagaaaccc
     2521 agcgaggtaa agacgagcca cgccgacatc tggccgagat cgtcgttgcc cgacagcccg
     2581 tcgggggtgg gcttatactg gctgtcgacg atctgcttca gccgctcctg cgtccgccac
     2641 ggcgcgccgg cataggcgta gagataggcg acatggtggc tcggctcgtt gccgtggata
     2701 tattggccga tcagccccgc gatatcctcg gcatggctgt aatcgagctt cgagatgtca
     2761 taatcgaaca tcgcgtcgag cttcgcgacc gtctttgcgt cgccgccaag gatgtcgaac
     2821 agcgccgcct gatcctgcgg cacgaaccag ctatattgcc aggcattgcc ctcggtatag
     2881 tccgagccat agttgatcgc ggtcggatcg aagggggtgc ggaagctgcc gtcggccttg
     2941 cgcgcgcgca gccagccggt cttcgcatcg aagctgttcc gccaatagcc ggcgcgcttg
     3001 tagaagcggt cggcgatgtc cttgcggccc atcttttcgg ccatccgcgc gatcgtccag
     3061 tcgtcatagg catattcgac ggtcttcgac gccgcctcgg gctcgcggtc gatcgcgaca
     3121 tagccgagcg tcatatagtc gccgagcccg ccataggggg cgtattcggc gctcgcgatc
     3181 atcgcgtcca aggcttcgtt cgcgtcgaaa tcgcccacgc ccttcagata ggcgtcggcg
     3241 atgaccggga cggcgtgata gccgatcatc gtccaggtct cgcggccgtg gaactgccag
     3301 acgggaagga tgccgtgcgg gctcgccttg cggctcgcga cgagcgattt gacgatgtcg
     3361 gtgttggtct gatcgggcgc gacgagcgtc agcagcggat gctgcgcgcg gaacgtatcc
     3421 cagagcgaga aggtcgagcg gaaggtgaaa tccttcgcgc tgtggaccgc atcgtcgggg
     3481 ccgcgatagc gtccatcgac gtcgctccac acgctcgggg cgagcaggct gtgatagagc
     3541 gcggtgtaga ggttggagcg catcggcgcg ggcgcttcga tttcgagcgc gccgagcgcc
     3601 ttgcgccatt cgccttccgt gcgggcgcgg actgcgtcga aaccggcgcc ccccgccttc
     3661 ttttcggcgg cgtcgaggtt ggcgaccgcg cccgcttcgt cgacgcccga gagcgcgacc
     3721 cggacttcga gcgggccggc gagagcgccg aaatcgagcc gcacttcgag tgccttgccg
     3781 agcttttcgg cgagcgcgtc ggtgccgcgg ccgggagggt ggaaaccctt gtagggaatc
     3841 ttctcgtcgc gatcgagaaa ggcgtgggac ttgagcggcg ccgagaagcg catcgcgaag
     3901 aagagtttgc gcccgggcgc ccagccgcgc gtctcgcgga aaccggtgag cgtgccgtcg
     3961 gggcgcaggt gcagccccga ccagagaatc ttgcccggat aatcgtagag cgacgagcgg
     4021 agatcgagga gcagatgcgc ctctttcccc gcggggaagc tgtagcggtg gacgccgacg
     4081 cgggtgcccg cggtgagttc ggcgcggatg ccgctgtcgg cgagcgtgac agcataatag
     4141 ccggggctcg ccttttcact gtcgtggctg aatcgctggc gatagcccga gccgggttgt
     4201 ttgggatcgc cgggatcgag cttcacctct ccaacctgcg gcatgaggag gatgtcgccg
     4261 agatccgaat ggcccgcgcc cgagaaatgc gtgtggctga acccctggat cgtcgggtcg
     4321 tggtagctgt agcccgcggc atgatcgtag cagtcgcgga tctggcacgt cgcgtcggtg
     4381 tcgggcgaca gctggaccat gccgaagggc gcggtcgcgc cggggaaggt atggccttcg
     4441 ggaccggtgc cgatcatcgg atcgacgaag gcgagcggat cgggcgccgg ttgctcggcg
     4501 aaggactgga tcggcaggag agcgagcagc gcggcggcgg cggtcaggcg tttcatcctg
     4561 cctttgtgcc gcgaaggcac cgccctgtcc aacggcgcgc ggcccgcccg gttcacgctg
     4621 cgaccccgac cgcgtccatc gccagcgcga tcgcgccaag cggacccgcc atcgttccga
     4681 gctcgggagc gatcacataa gcgccatttc ctggaatctg catatagtcc gcgaggctct
     4741 tttcgagctg ggcgttgatc cgcggcagca attgcggctg gcccgcgagc accccgccgc
     4801 ccatcgcgac gcgcatcggc ccggtcgtgc a
//
LOCUS       DATMSU010000300         6041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_147035,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000300 DATMSU010000000
VERSION     DATMSU010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147035"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VN034_08275"
     CDS             complement(<1..216)
                     /locus_tag="VN034_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HWV60633.1"
                     /translation="MSAQSPLDDFKAALSSVARAVTRDAEVEVGFTADAPAQIGKAIK
                     VPTPSRTLPVDQVAEARGFADAYALRMK"
     gene            449..1642
                     /locus_tag="VN034_08280"
     CDS             449..1642
                     /locus_tag="VN034_08280"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60634.1"
                     /translation="MKRPWIVIACAAFIVTLAMGVRQSFGLFLPQMSVDIGISRSDFG
                     LAMALQNLLFGLVQPFVGALADKHGAGRVVFFGALLYALGLIGAAFATDAIGLHISFG
                     IFIGMAQSATTFVVVLGAVGRVVSPEKRSGAFGIVTAGGSLGQFLVVPLASMLLGDVG
                     YHQTLWIMAGLVALCGLLAIGVAGRTDTPGTMQAEEQSARDALREAAAHRGYWLLNAG
                     FFVCGFHIAFIATHLPAYLDDKGLGIEIGAQVLALVGLFNILGSYVFGRAGDLLRQKY
                     VLSALYTARSAVIALFLFLPLSHASALIFAGAMGFLWLGTVPLTSGIVGRIFGIRYLS
                     MLYGIVFLSHQLGSFFGAWMAGLIFDATGSYNIAWGFSIALGLLAGVVHLPIADAPVK
                     RLQSA"
     gene            1639..1770
                     /locus_tag="VN034_08285"
     CDS             1639..1770
                     /locus_tag="VN034_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60635.1"
                     /translation="MKLARQDWIALALFVAVALGGLWLWTGEGPVVWLTNFAIMCGF"
     gene            complement(1774..2967)
                     /locus_tag="VN034_08290"
     CDS             complement(1774..2967)
                     /locus_tag="VN034_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV60636.1"
                     /translation="MRTKSLLVLLALAALSGCSIAAVDYGKIRHAEYIADPRCTAQPG
                     AVVDGEALPFFFATSRLPDCRTPEIQLLHHRGDRIRYGRFNAPRDVEVGKKERFLTPL
                     AFQTPADWWRALQAETDRRQGRVLLYVHGYRENFSTTSKDAAQIARMTGFDGPIVEYS
                     WPSQGKILSYVVDETNMYHDVRNFRDFLKSLAEQSWVKEIVVVSHSLGARLVIPAIAY
                     VDRTSSSADSSNISNVILASPDIDRETFERDIEEEVLSARRVAVGRRITAYVSRADKA
                     LAASRAIHGYPRLGSPYCFNPFEAAELKAKGLPERCYPESRPGLTVIDTTDVSRGSTG
                     HSNFLRSAVACRDFIDVVANKRTRPERVATQWAHVFRLVPDPAVPEDGDDAICHRTSE
                     AEAPE"
     gene            3134..4201
                     /locus_tag="VN034_08295"
     CDS             3134..4201
                     /locus_tag="VN034_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5694 domain-containing protein"
                     /protein_id="HWV60637.1"
                     /translation="MTAFTALLLTMSGAAHPVYVPPFDPRQLKDNVAGTPGELLVLGS
                     PHLSGLPDSFKPESLEPLLARLARWSPAIITIEGLSGTDCEFLVRYKPLYPGAADYCW
                     DPGTAQKIVGLDMVAATVEADRLLGSWPTNPTPADRRRLAALFLAGGERPSALVQWLR
                     LPPSERRAGDGLDATLVALLDKAMTARNENFLIGSALAARLGHERVYAIDDHSADGVT
                     ASLDKDPGYGAALQRIWQTPQLKDRIAAEKQLEANLDGEGVLALYRFYNRPATMRLAF
                     DTDFGAALADTSAQQYGRRYTGWWETRNLRMVANIRAAMAVRPGARTLAIVGASHKGY
                     FEAYLNLMHDVRLVDAATILK"
     gene            complement(4206..5195)
                     /gene="cobS"
                     /locus_tag="VN034_08300"
     CDS             complement(4206..5195)
                     /gene="cobS"
                     /locus_tag="VN034_08300"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HWV60638.1"
                     /translation="MTDIPNSLPDHHGSTLLAAPDVEVDARELFGVDIDMKVPAFSEA
                     DERVPDLDPAYVFDGDTTLAILAGFKYNRRVMVQGYHGTGKSTHIEQVAARLKWPCIR
                     VNLDAHISRIDLVGRDAIVLRDGQQVTEFREGLLPWALQTPTALVFDEYDAGRPDVMF
                     VIQRVLETEGKLTLLDQNRVIRPNPHFRLFSTANTVGLGDTSGLYHGTQQINQGQMDR
                     WNIVVTLNYLPAATESAIILAKVPNTDETTVANMVKVADLTRQGFINGDISTVMSPRT
                     VISWAQNTAIFNNIGFAFRLSFLNKCDEAERMIVAEYYQRVFGEDLPESVVGK"
     gene            complement(5289..5726)
                     /locus_tag="VN034_08305"
     CDS             complement(5289..5726)
                     /locus_tag="VN034_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV60639.1"
                     /translation="MTDLNEARIEVVRRHMALEITHDWDGVLATFDHPRYELMGPGTI
                     FDGEAAVRSYFAASREPFPDQANEVIAIAADETTNTVLVEFWLSGTHTGPLKLGLRTI
                     EPTGKAFRIRMAASFEFAPGGDKIVCERPYYDQSAVMKALGLF"
     gene            complement(5738..>6041)
                     /locus_tag="VN034_08310"
     CDS             complement(5738..>6041)
                     /locus_tag="VN034_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWV60640.1"
                     /translation="LPPRWADFSDPMDALGARFRQRMDEARREAANPRLSREEHDAMQ
                     LLGLPADADRAALRRRYSELVRKYHPDRNGGDRSFETRLGAVVEAYQLLRKSKAFA"
BASE COUNT          978 a         1911 c         2027 g         1125 t
ORIGIN      
        1 cttcatccgc aaagcataag cgtcggcaaa gccgcgcgct tcggcgacct gatcgacggg
       61 cagcgtgcgc gacggcgtcg gcaccttgat cgccttgccg atctgcgcgg gcgcatcggc
      121 ggtaaagccg acctcgacct cggcatcgcg cgtcaccgcg cgcgcgacgc tcgacagcgc
      181 agccttgaaa tcgtcgaggg gagattgggc ggacatcgag tcggctctaa ccagctttgg
      241 gggacgaagg aagaggatgc cgtcacgcat gccataatca tcaccccgga cttgatccgg
      301 gatcccgctt ttcggcgccg ctgagcggga ccccggatca agtccggggt gacgaaagga
      361 ggagtaagcg gggctgtagc taagctcgct tgacggacgc ggaaggcggg cgcatcgccg
      421 cgacatgagc ccccctgcac tctcccccat gaagcgccca tggatcgtga tcgcctgcgc
      481 cgcgttcatc gtcacgctgg cgatgggtgt gcgtcagtcg tttggtctgt tcctgccgca
      541 gatgagcgtc gatatcggga tcagccggtc ggacttcggg ctcgcgatgg cgctccagaa
      601 cctgctcttc ggcctcgtgc agcccttcgt cggcgcgctc gccgacaagc atggtgcggg
      661 gcgcgtcgtc tttttcggcg cgctgctcta tgcgctcggg ctgatcggcg ccgccttcgc
      721 gaccgatgcg atcgggctgc atatcagctt cggcatcttc atcggcatgg cgcagtcggc
      781 aacgaccttt gtcgtcgtac tgggcgcggt cggccgcgtc gtcagcccgg aaaagcgcag
      841 cggcgccttc ggtatcgtca ccgcgggcgg gtcgcttggc cagttcctcg tcgtgccgct
      901 cgcgtcgatg ctgctcgggg acgtcggcta tcaccagacg ctgtggatca tggccgggct
      961 cgtcgcgcta tgcgggctgc tcgcgatcgg cgtcgcgggg cgcaccgaca cgccgggtac
     1021 gatgcaggcc gaggagcaga gcgcgcgcga cgcgctccgc gaagccgcgg cccaccgcgg
     1081 ctattggctg ctcaacgcgg gcttcttcgt ctgcggattc cacatcgcct tcatcgcgac
     1141 gcatctcccc gcctatctcg acgacaaggg gctggggatc gagattggcg cgcaggtgct
     1201 cgcgctcgtc gggctgttca acatcctcgg ctcttacgtg ttcggccgcg cgggcgacct
     1261 gctgcgccag aaatatgtgc tgtcggcgct ctacaccgcg cgctcggcgg tgatcgcgct
     1321 cttcctcttc cttccgctca gccatgccag cgcgctcatc ttcgcgggcg cgatgggctt
     1381 cctctggctc ggcaccgtgc cgctcaccag cgggatcgtg gggcgcatct tcggcattcg
     1441 ctatctctcg atgctctacg gcatcgtctt tttgagccat cagctcggca gcttcttcgg
     1501 cgcatggatg gcggggctga tcttcgacgc gaccggcagc tacaatatcg cctggggctt
     1561 ctcgatcgcg ctcgggctgc tcgcgggggt ggtccacctg ccgattgccg acgcgcccgt
     1621 caaacggctg caatcggcat gaagctggcg cggcaggact ggatcgcgct cgcgctgttc
     1681 gtcgctgtcg cgctcggcgg gctgtggctc tggaccggcg aggggccggt cgtctggctg
     1741 accaattttg cgatcatgtg cggtttctag ccgctattcg ggcgcctcgg cttcggacgt
     1801 ccggtgacag atcgcatcgt cgccgtcctc tggcacggcg gggtcgggga ccaagcgaaa
     1861 cacatgcgcc cattgggtgg cgacccgctc gggccgcgtg cgcttgttcg cgacgacatc
     1921 gatgaaatcg cggcacgcga ccgcgctgcg gaggaaattg ctgtgcccgg tcgatccgcg
     1981 cgaaacgtcg gtcgtgtcga tcacggtcag ccccgggcgc gattccggat agcaacgttc
     2041 gggtagtcct ttggctttca gctccgccgc ctcgaacggg ttgaagcaat agggcgaacc
     2101 gagccgcgga tagccgtgga tcgcgcgcga ggctgcgagc gccttgtcgg cgcgcgagac
     2161 ataggcggtg atccggcggc ccaccgccac acgccgcgcc gacagcacct cttcctcgat
     2221 atcgcgctcg aacgtctcgc ggtcgatgtc gggtgaggcg aggatgacgt tcgagatatt
     2281 gctgctgtcg gcgctgctcg acgtgcggtc gacataagcg atggcgggga tcaccagccg
     2341 ggcaccgagc gagtgcgaaa cgacgacgat ctccttgacc cagctctgct cggcgagcga
     2401 tttcaggaaa tcgcgaaagt ttcggacgtc gtgatacatg ttcgtctcgt cgacgacata
     2461 gctcaggatc ttgccctgcg atggccagct atattcgacg atcggcccat cgaagcccgt
     2521 catccgtgcg atctgcgccg cgtccttcga cgtcgtcgag aaattctcgc gatagccgtg
     2581 gacatagagc agcacgcggc cctgcctgcg gtcggtctcg gcctgcagcg cgcgccacca
     2641 gtcggccggc gtctggaagg cgagcggcgt aaggaagcgt tccttcttgc cgacctcgac
     2701 atcgcgcggc gcgttgaagc ggccgtagcg aatgcggtcg ccgcggtggt gcaggagctg
     2761 gatttcgggg gtgcggcagt cgggcaggcg acttgtagcg aagaaaaaag gcagcgcctc
     2821 gccatcgacg accgcccccg gctgcgcggt gcagcgcggg tcggcgatat attcggcatg
     2881 gcggatcttg ccataatcga cggccgcgat gctgcatccg gacagggcgg cgagggcaag
     2941 gaggacgagc agggatttcg ttcgcatatg tagcttcatc tttgaaaaac ctgacgcatc
     3001 gcccgacgct ggcagaggag agcgtacatg ggaagccgga aaatcgctcc cgcgaagagt
     3061 gttgatagaa taatacactt ggcctattcc cgtccagtgt cgttggtgcg gcgtgtttat
     3121 tggggagggg ttcgtgacgg ctttcaccgc cttgttgctg acgatgagcg gggctgcgca
     3181 tcccgtctat gttccgccat tcgacccgcg ccagctcaaa gacaatgtcg cgggaacgcc
     3241 gggcgaattg ctggtccttg gttcaccgca tttgtcggga ttgcccgaca gcttcaaacc
     3301 cgaaagcctc gaaccgctgc tcgcgcgtct tgcgcggtgg agccccgcga tcatcacgat
     3361 cgaagggctt tcggggaccg actgcgaatt cctggttcgc tacaagccac tctatccggg
     3421 cgccgccgat tattgttggg atcccggcac cgcacagaag atcgtcgggc tcgacatggt
     3481 cgccgcgacg gtcgaggccg acaggctgct cggcagctgg cccacgaatc cgacgccagc
     3541 cgatcgccgc cgtcttgccg cactgtttct ggcgggaggc gaacggccat cggcgctggt
     3601 ccagtggctg cggcttccgc cgagcgaaag gcgtgcgggc gatggcctcg acgcgacgct
     3661 cgtcgcgcta ctcgataagg cgatgacggc gcgcaatgaa aacttcctga tcggatcggc
     3721 gctcgccgcg cggctcgggc acgagcgtgt ctatgcgatc gacgaccata gcgccgacgg
     3781 cgtcacggcc tcgctcgaca aggacccggg ctatggcgcg gcgctgcagc ggatatggca
     3841 aacgccgcag ctcaaggaca ggatcgctgc ggaaaagcaa ctggaggcca atcttgacgg
     3901 cgagggggtg ctggcgctct atcgcttcta caatcggccg gcgacgatgc gtctcgcctt
     3961 cgacaccgat ttcggggccg cgctcgccga caccagcgca cagcaatatg ggcgccgcta
     4021 caccggctgg tgggaaacac ggaacctgcg catggtcgcg aacatccgcg ccgcgatggc
     4081 ggtccggccg ggcgcacgca cgctcgcgat cgtcggcgcc tcgcacaagg gctatttcga
     4141 ggcgtacctc aacctgatgc acgacgtccg gctggtcgat gccgcgacca tcctgaaatg
     4201 acggcttatt tgccgaccac gctctcgggc aggtcctcgc cgaacacgcg ctgataatat
     4261 tcggccacga tcatccgttc ggcctcgtcg catttattga ggaaggagag gcggaaggcg
     4321 aagccgatat tattgaaaat cgcggtgttc tgcgcccagc tgatcacggt gcgcgggctc
     4381 atcacggtcg agatgtcgcc gttgatgaag ccttggcggg tcaggtcggc gaccttcacc
     4441 atattggcga cggtcgtctc atcggtgttc ggcaccttgg cgaggatgat cgcgctctcg
     4501 gtcgcggcgg ggagatagtt cagcgtgacg acgatgttcc agcggtccat ctggccctgg
     4561 ttgatctgct gcgtgccatg atacagcccg ctcgtgtcgc cgagcccgac ggtgttcgcg
     4621 gtcgaaaaca ggcggaaatg cgggttcgga cggatgacgc ggttctggtc gagcagggtc
     4681 agcttgccct cggtctccag cacgcgctgg atcacgaaca tcacgtcggg acgccccgcg
     4741 tcatattcgt cgaagaccaa tgccgtcggg gtctgcagcg cccaggggag caggccctcg
     4801 cggaattcgg tgacctgctg gccgtcgcgc agcacgatcg cgtcgcggcc gacaaggtcg
     4861 atgcggctga tatgcgcgtc gaggttgacg cggatgcacg gccatttgag gcgcgccgcc
     4921 acctgttcga tgtgcgtcga cttgccggtg ccgtgatagc cctgcaccat cacgcggcgg
     4981 ttatatttga agcccgcgag gatcgcgagc gtcgtgtcgc cgtcgaagac ataggcgggg
     5041 tcgaggtcgg ggacgcgttc gtcggcctcg ctgaacgccg gcaccttcat atcgatatcg
     5101 accccgaaca gctcgcgcgc gtcgacctcg acatcgggcg ccgcaagcag cgtcgagccg
     5161 tgatgatcgg gaaggctgtt cggaatatcg gtcatggcaa agtccagcaa gcaaacaggg
     5221 gagagtcgcc gccgggcggc gcttcggctg tcgcggtata gcgacgggtt gcgccttgca
     5281 acgccttttc agaagagccc gagcgccttc atcaccgccg actggtcgta ataggggcgc
     5341 tcgcagacga tcttgtcgcc gcccggcgcg aattcgaagc tcgccgccat gcggatgcgg
     5401 aacgccttgc cggtcggctc gatcgtgcga aggccgagct tcagtgggcc ggtgtgcgtg
     5461 ccgctgagcc agaattcgac gagcacggtg ttcgtcgttt cgtcggcggc gatcgcgatc
     5521 acctcgttcg cctgatcggg aaagggctcg cgcgacgcgg cgaaatagga tcgcaccgcc
     5581 gcctcgccgt cgaagatcgt gccggggccc atcaattcgt agcgcggatg gtcgaaggtc
     5641 gcgaggacgc cgtcccagtc gtgcgtgatc tcgagcgcca tatggcggcg caccacctcg
     5701 atccgtgctt cgttcaggtc ggtcatgacg cctctcctta tgcgaacgcc ttgctctttc
     5761 tcagcaattg ataggcttcg actaccgcac ccagtcgcgt ttcaaaactg cgatcgccgc
     5821 cattgcggtc gggatgatat ttgcggacga gctcgctgta gcggcggcgg agcgccgcgc
     5881 ggtcggcgtc ggcagggagg ccgagcaact gcatcgcgtc atgttcctcg cgcgacaggc
     5941 ggggattggc cgcctcgcgc cgcgcctcgt ccatccgctg gcgaaaccgc gcgccgagtg
     6001 catccatcgg gtcgctgaaa tcggcccagc gcggcggcag a
//
LOCUS       DATMSU010000301         4448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_119221,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000301 DATMSU010000000
VERSION     DATMSU010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119221"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1745
                     /locus_tag="VN034_08315"
     CDS             117..1745
                     /locus_tag="VN034_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWV60641.1"
                     /translation="MSIFSWMNPDVPPRDQSVLRYALERWEVERGEQAYAVFPGGGRW
                     SFAEMGRQARLVATGLARLGVARGDRVMSWLPNGPDALRVWFGINWLGAVYAPINLAY
                     RGSILRNAVEIAEPKLIICHSSLVSRLDEAEIDPALPAVLIDRRDVDAKGARPLLGGD
                     CLDAGIDTALLRLDEPIEPWDEQAIIYTSGTTGPSKGVLSSYCHGATAALVAFEEKSR
                     ADLRYLVTLPLFHAGGTIGTIGTLLLGHSIALPEQFETGRFWDVVRETRSTCCTLLGA
                     MAGFLNKQASAPGERDHGLQWAYVLPHGQTATDFSARFGVATRTMFNMTELAVPILSR
                     EGQADAESCGRARVGVTLRIADAQDREVASGEVGELLVRADRPWSTSHGYAGNAEATA
                     AVWRNGWFHTGDLFRRSADGDYYFVDRAKDSIRRRGENISSYEVERECLSFGAVREAA
                     AYAVASEFGEDEVMVALSSNPGAPIDPAALVDHLRVRMPYYMVPRYIRILDDLPKTPT
                     AKILKTELRAEGRTADVWDREAHGIILKRDRING"
     gene            complement(1735..2025)
                     /locus_tag="VN034_08320"
     CDS             complement(1735..2025)
                     /locus_tag="VN034_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60642.1"
                     /translation="MRDPVGAQARDVIAVRDIETAAFVDQIVDCRMGIGGEPAAIVFK
                     VHPCPPRQGLGEFVEVIGRGDGKAGKIGFPHVAVAMDRADVDDAGPGATVSH"
     gene            2029..2727
                     /locus_tag="VN034_08325"
     CDS             2029..2727
                     /locus_tag="VN034_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV60643.1"
                     /translation="MLHGFPWRGRSGADANRSIARLAAMAPDLLTCWLGVSLRAPDEA
                     ASQIECALRDWNIGGVSVSPFWEAVPASDPALAPIFETAQRLNLPIWIHAGQNFNRSI
                     SLDISDVRNIDRVATAFPDLTILIGHGGWPWISDAVAIVQRHPNVHLELSSHSPRFMG
                     RAGSGWEPLLAQARGALRGRVLFGASAWVSRLGPAGLAAQIDELGLPADIAAEWRHDN
                     AAWLLAGAGRKAMD"
     gene            2934..4133
                     /locus_tag="VN034_08330"
     CDS             2934..4133
                     /locus_tag="VN034_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60644.1"
                     /translation="MLLLVYTFNFIDRQILGILAVPVQAELGLSDTQLGLLGGIAFAL
                     LYSTAAIPLAWLADRTGRTWVITGSLAVWSGFTALCGLAGGFWSLFLCRLGVGVGEAG
                     GVAPSFSLISDYFPSAQRARALAIYSLGIPLGSALGVYFGGYIAANVDWRAAFIVVGL
                     AGVALAPLFRLIVRDPPRAAGAPDTMRLRGVLAILSRKRSFWFLALGAASSSIMGYGM
                     AFWLPTLLQRSFGLDLEGTSLFFGTMLLLGGGVGVLLGGVIGDRVGRKDKKALATVPA
                     FCFLVAAPVFAGGILSDSLPLTFVLFTIPMALGYVWLAPVIAAVQHLVPSQMRTTATA
                     SFLFINNLLGPGLGIYILGGLSDALLPVYGNDGLKYAMMGCLLFYVISAVMMLLGARS
                     LDHDWVD"
     gene            4130..>4448
                     /locus_tag="VN034_08335"
     CDS             4130..>4448
                     /locus_tag="VN034_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60645.1"
                     /translation="MKEVATIFAERHIMVGALKLRVLHAEGTQSQLRKAFPTRSCLLA
                     ARPGVSFFRLAPESGPSAIDDVDGSPTSRGMIRGMSWKESYDGEDCNSRVGYREVGVS
                     GPRR"
BASE COUNT          705 a         1368 c         1454 g          921 t
ORIGIN      
        1 cgccatgttg caggaagccg cgcaccagac ctgttttttc gaaaccgaag atgcgaggga
       61 aggtgccagc gcctttatcg agaaccgcaa gccgacattt acgggcaggt gaaccaatga
      121 gcattttcag ctggatgaac cccgacgttc cgccgcggga ccagtccgta ttgcgctatg
      181 cgctcgagcg atgggaagtc gaacgcggcg aacaggctta cgccgttttt cccggcggcg
      241 ggcggtggag ctttgccgaa atgggccggc aggcccgtct ggtcgcgacg ggactggcgc
      301 gcctcggcgt cgcgcgaggc gaccgcgtga tgagctggct tcccaacgga cccgacgcct
      361 tgcgcgtttg gttcgggatc aactggctgg gtgcggtcta cgcgccgatc aatcttgcct
      421 atcgcggctc gatcctgcgc aatgcggtcg agattgcgga gccgaaactg atcatctgtc
      481 attccagcct tgtttcgcgg cttgacgagg cggagatcga cccggcgttg ccggcggtgc
      541 tgatcgaccg gcgcgacgtc gatgcaaagg gagcaaggcc gttgctgggc ggggactgcc
      601 ttgatgccgg catcgacacg gcattgctcc ggctggacga gcccatcgag ccctgggacg
      661 agcaggcgat catatataca tcgggaacga ccgggccatc gaagggggtg ctcagttcct
      721 attgccacgg cgcgacggcc gctctcgtcg cgttcgagga aaagagccgc gccgacctgc
      781 gctatcttgt gaccttgccc ctgtttcacg ccggcgggac gatcggaacc atcggcacgc
      841 tgttgctggg gcactcgatc gcgctgcccg aacagttcga gaccgggcgt ttctgggacg
      901 tggtacgcga aacgcggtcg acctgctgca cgcttctggg cgcgatggcg ggatttctga
      961 acaagcaggc gtcagcgccc ggtgaacggg atcacgggct gcaatgggct tatgtcctgc
     1021 cgcacgggca gacagctacg gacttttccg cgcgctttgg cgttgccacc cggaccatgt
     1081 tcaacatgac cgaactggcg gtgccgatcc tgtcacgcga aggtcaggcc gatgccgaaa
     1141 gctgcgggcg cgcgcgcgtc ggcgtgacct tgcgtatcgc agatgcgcag gatcgggagg
     1201 tcgcgtccgg cgaggtcggc gaactgctgg tccgtgccga ccgcccgtgg tcgacgtccc
     1261 atggctacgc cggcaatgcc gaggcgaccg ccgcggtctg gcgcaatggc tggtttcaca
     1321 cgggcgatct gtttcgccgc tcggccgacg gcgattatta tttcgtcgac cgcgccaagg
     1381 attcgatacg ccggcggggg gaaaatattt cctcctacga ggtcgaacgc gaatgcctgt
     1441 cgttcggtgc ggtccgcgaa gccgccgcct atgccgtcgc cagcgaattc ggcgaagatg
     1501 aggtcatggt ggcgctatcc tccaatccgg gagcgccgat cgatccggcc gccctcgtcg
     1561 atcatctgcg cgtccggatg ccctattaca tggtgccgcg ctacatccgt atcctcgacg
     1621 atcttcccaa gacaccgacc gcgaagatcc tgaagaccga actgcgcgcc gaagggcgaa
     1681 cggcggacgt ctgggatcgc gaggcgcacg gcatcatatt gaagcgggat cgcatcaatg
     1741 gctgacggta gcgccgggcc cggcatcatc gacatctgcg cggtccatgg cgaccgcgac
     1801 gtgtgggaag cctatcttgc cggctttgcc atcgccgcgc ccgattacct cgacaaattc
     1861 gccgagcccc tgacgcggcg ggcaggggtg gaccttgaag actatcgccg ccggctcgcc
     1921 gccgatcccc attctgcagt ccactatttg atcgacaaag gcagcggttt cgatgtcgcg
     1981 gacggcgatt acatcgcggg cctgcgcgcc gacgggatca cgcatcaggt gctgcacggc
     2041 ttcccctggc gcgggcggtc cggcgccgat gccaatcgat cgatcgcccg cctcgcggcg
     2101 atggcgccgg acctgctgac atgctggctg ggcgtttcct tacgcgcgcc cgacgaagcc
     2161 gcctcgcaga tcgaatgtgc gctgcgcgac tggaatattg gcggggtgtc ggtttcaccc
     2221 ttctgggagg ctgtcccggc atctgacccg gcgctggcgc cgatattcga gacggctcaa
     2281 cggctcaatc tgccgatctg gattcatgcc ggacagaatt tcaatcgctc catctcgctc
     2341 gatatttcgg atgtacggaa tatcgatcgc gttgcgacgg cgtttcccga tttgacgatt
     2401 ttgatcggtc acgggggatg gccgtggata tcggatgccg tcgcgatcgt gcagcggcac
     2461 cctaacgtcc atctcgaact gtccagccac agcccgcgct tcatgggccg tgcggggtcg
     2521 ggttgggagc ccttgctcgc acaggctcgc ggcgcgttgc gtggtcgcgt cctgttcggg
     2581 gcctccgcct gggtttcccg gctgggaccc gccggcctcg ccgcgcaaat cgacgaactg
     2641 ggattgccgg ccgatatcgc cgccgaatgg cgccatgaca acgcggcgtg gcttttggcc
     2701 ggcgcgggcc ggaaggcgat ggattgagat agtcggcaca cgctgctgca taccgaggat
     2761 catgcaatgg agcctgtagc ggacgcccgc gcgccagagc gcaccaaagg aaaatcgacc
     2821 ggccaagcgg tctgccatga aggaaatgcc gcgtgaaaaa gggtctgtca gtttctccgg
     2881 ccgatgcagc cgaaggccat gggtcgagct cctatcgggg gcttgttctc ctgatgctcc
     2941 tccttgtcta caccttcaat tttatcgacc gtcagatatt gggcatcctc gccgtgcccg
     3001 ttcaggcgga gctgggcctt agcgacacgc agctcggcct gctcgggggg atcgcctttg
     3061 ccttgctcta ctcgactgcg gccattccgc tggcttggct cgccgatcgc acgggtcgca
     3121 cctgggtgat caccggctca ttggcagtct ggagcggctt caccgccttg tgcggcctgg
     3181 cgggcggatt ctggtcgctc ttcctttgcc ggctcggcgt aggggtgggc gaggcggggg
     3241 gcgtcgcacc gtccttttct cttatctcgg attatttccc gtcggcacag cgcgcgagag
     3301 ctctcgcaat ctattcgctc gggatcccgc tgggctcggc tttgggcgtg tatttcgggg
     3361 gctatatcgc agcgaacgtc gactggcggg ccgctttcat tgtcgtggga ttggccggag
     3421 tcgcgctggc ccccctgttt cggctgatcg ttcgggaccc tcctcgcgct gcgggcgcgc
     3481 ctgacaccat gcgccttcgg ggcgttctgg ccatcctgtc gcgaaagcgc agtttctggt
     3541 tcctcgccct cggcgcagcg tccagttcga tcatgggcta tggcatggcg ttctggctcc
     3601 cgacgctcct gcagcgcagc tttggcctcg atctggaggg gacatcgctc ttcttcggga
     3661 ccatgctgct gctcggcggc ggtgtcggtg tgctgcttgg tggcgtgatc ggcgaccgcg
     3721 ttggccggaa ggacaaaaaa gcgctcgcga cggtgcccgc cttttgcttt ctggttgcgg
     3781 cgcccgtctt cgccggcggc atattgtcgg actcgcttcc gctgaccttt gtcctgttca
     3841 ccatcccgat ggcgttgggc tatgtgtggt tggcgccggt cattgcggca gtccagcatc
     3901 tcgtgccgtc gcaaatgcgc accactgcca cagcaagctt cctgttcatc aacaacctgc
     3961 tcggccccgg gctcggtatc tatatattgg gagggctctc ggacgcgttg ctgccggttt
     4021 atggcaacga tggtctcaaa tatgcgatga tgggttgcct gctgttctac gtcatttcag
     4081 cggttatgat gctactcggc gcccggtcac tcgatcacga ttgggtcgat tgaaggaagt
     4141 tgcgaccata tttgccgaac gccacattat ggtcggcgcg ctgaagctgc gggttttgca
     4201 tgctgagggc acgcaatctc aacttcgcaa ggctttccca actcggagtt gtctgctcgc
     4261 ggcgcgacca ggcgtcagct ttttccgact tgcacccgaa agcggaccgt ccgcaattga
     4321 tgacgtggac ggctctccaa cctcccgcgg tatgatccgc gggatgagct ggaaggagag
     4381 ctacgatgga gaagattgca acagtcgggt tggatatcgc gaagtcggtg tttcaggtcc
     4441 acggcgtt
//
LOCUS       DATMSU010000302         3542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_43455, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000302 DATMSU010000000
VERSION     DATMSU010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43455"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..589
                     /locus_tag="VN034_08340"
     CDS             152..589
                     /locus_tag="VN034_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV60646.1"
                     /translation="MRFASRPSCIKRLLIIEDDPLTAFDNEHTLKHGGYHIVATVDSG
                     EAAVAVMAAEPVDALVLDLGLAGHMSGREVARLARDRGIAVLLVTGQCPADASEIALA
                     CLGKPHSASALVAALKALETMICKNEAPRKVSGLTTFWRPEAA"
     gene            702..2132
                     /locus_tag="VN034_08345"
     CDS             702..2132
                     /locus_tag="VN034_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60647.1"
                     /translation="MQFGLDSVVLVAVLALVIGLAFVIPSFAPYRKPMPIVAFLLGIS
                     SGFPLTLLLGTMTFWLSKVGIEKSTIGFAIGLTTPYTLKFLWAPLVDRLPLPFLTKTF
                     GQRRGWLFFIQALLVVAIWQLGTSNPTNDNLAAFAFWAIVVAFLSATQDIVIDAYRIE
                     ILSDAELPHGTAMNQFGYRTGNLIAGVGTIALASPEGFNLGWAAAYGFTGLAILPALL
                     GALYAGPGRFDPSRARAAGQSFGSWLQDTVVNPFREFFTRHGAVLILLFVLVYKVGDA
                     MGQGMLNPMIVELGFTDTEFVAINKGVGFVALIIGSAVAAPFIAWLGMGRALLVSGLL
                     MMFSNLLFAVLASAGHSPLMLAIAVGTENFTSGIGLTVFVTYLSGLSSLAYTATQFAL
                     LSSFAAVGRTWLAAPGGYIAEGLGWVGFWLFTVAIAVPGMLLLWILWKKGFVVQTVRQ
                     PSTEDDGHEVAHPEEDKPIPAAPGSR"
     gene            complement(2104..3420)
                     /locus_tag="VN034_08350"
     CDS             complement(2104..3420)
                     /locus_tag="VN034_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HWV60648.1"
                     /translation="MPDHAHSSDPAVQTQLDRLAALSPGRDILGLERIAELCARLGNP
                     QLQLPRTFHVAGTNGKGSTCAYLRAILEAQGRRVHAYTSPHLVRFNERIRLAGTLVDD
                     ALLAALLEEVLDEAADLQASFFEVTTAAAFLAFARIPADDCIIEVGLGGRLDATNIIP
                     PPAAAGIASLGIDHEAFLLAPEAGTPDDPAMRIAWEKAGIIKPETPVATLAYPPHIRD
                     TIAAKVADVGATLFPEGDGWAVEAEGEAFHWTSKLGSVAPLLHPRMAGAHQMRNAGLA
                     IAMLRLAPDPRPTDADIAQGVANAFWPARMQRLGAGPLADLLPDGTEIWLDGAHNVDA
                     GFALARQFGSEKRRIHLITGMLANKDPAAIIAPLADRLASLTVVPVPGHEYHSAAAFG
                     PGAKAAASIPVALSGLDVDLAREIVLIAGSLYLAGEVLSANRELPE"
BASE COUNT          555 a         1077 c         1244 g          666 t
ORIGIN      
        1 aacggatgcg gctttcttgt cgttttcaag ggagaagtgt tgcgttgtga tcacgcccat
       61 agccggaaaa gacccgcttc gtcgatggca agcagaagcg atcttgcggg gcgccgccga
      121 gcgagtcatg gcaacgtcaa agggagaaac catgcgcttc gcgtcccgcc cgtcctgtat
      181 caagcggctc ttgatcatcg aggatgatcc gctgaccgct ttcgacaatg agcatacgct
      241 gaaacacggc gggtatcaca ttgtcgcgac ggtcgattcg ggcgaggcgg cggtggcggt
      301 catggcggcc gagccggtcg atgcgcttgt gctcgacctg ggactcgcgg ggcatatgtc
      361 cgggcgcgaa gtcgcgcggc tggcgcgcga ccgcgggatc gcggtgctgc tggtgacggg
      421 acaatgcccc gccgacgcga gcgagatcgc gctcgcctgc ctcggcaagc cgcacagcgc
      481 gtcggcgctc gtcgcggcgc tgaaggcgct cgaaacgatg atctgcaaga atgaggcgcc
      541 ccgcaaggtg agcgggctga cgaccttttg gcgacccgaa gccgcgtgag cgcgacttgg
      601 gtcctagcct tggccgataa atcaccgctt ccaatttggg atcaagcctc taggccttga
      661 ggcgcaccat ccgcgtaggc ggagcagggg aggttgttcg gatgcagttt gggctcgatt
      721 cggtggtgtt ggtggcggtg ctcgcgcttg tcatcggcct tgctttcgtg atcccgagct
      781 ttgcgcccta tcgcaagccg atgccgatcg tcgccttttt gcttggcatt tcgagcggct
      841 ttccgctcac tctgctgctc ggcacgatga ccttctggct gtcgaaggtc gggatcgaga
      901 agtcgacgat cggcttcgcg atcgggctga cgacgcccta tacattgaaa tttttgtggg
      961 cgccgctcgt cgaccgcctg cctctgcctt tcctgaccaa gacgttcggg caaaggcgcg
     1021 gctggctgtt cttcatccag gcgctgctgg ttgtcgcgat ctggcaattg gggacgagca
     1081 accccactaa cgacaatctg gcggctttcg ccttctgggc gatcgtcgtg gccttccttt
     1141 cggcgacgca ggacatcgtc atcgacgcgt atcgcatcga gatattgagc gacgctgaat
     1201 tgccgcacgg caccgcgatg aaccagttcg gataccggac gggcaatctg atcgcgggcg
     1261 tcgggacgat cgcgctggcg tcgcccgaag ggttcaatct cggctgggcg gccgcctatg
     1321 gctttacggg cctcgcaatc ctgcccgcgc tgctcggcgc gctttatgcg gggcccggac
     1381 gcttcgaccc gagccgcgcg cgggcggcgg gtcagagctt cggcagctgg ctgcaggaca
     1441 cggtggtcaa tccgttccgc gaatttttca cccgccatgg cgcggtgctg atcctgcttt
     1501 tcgtcctcgt ctacaaggtc ggcgacgcga tggggcaggg gatgctcaac ccgatgatcg
     1561 tcgaactggg gttcaccgat acagaattcg tcgcgatcaa caaaggcgta ggcttcgtcg
     1621 cgctgatcat aggatcggcg gtcgcggcgc ccttcatcgc ctggctcggc atggggcgcg
     1681 cgctgctcgt gtcgggcctg ttgatgatgt tcagcaacct cctgttcgcg gtgctcgcgt
     1741 cagccggcca ttcgccgctg atgcttgcga tcgcggtggg gaccgagaat ttcacgagcg
     1801 gcatcgggct gacggtgttc gtcacctatc tctcgggcct gtcgagcctt gcctataccg
     1861 caacgcagtt cgcgttgctg tcgtccttcg ccgcggtggg ccgtacgtgg ctcgcggcgc
     1921 ccggcggtta tatcgcagaa ggcctcggct gggtcggttt ctggctgttc acggttgcca
     1981 tcgcggttcc cggaatgctg ctgctctgga ttctctggaa aaagggcttc gtcgtgcaga
     2041 ccgtgcgcca gccgagcacc gaggacgacg ggcacgaggt cgcgcacccc gaagaggaca
     2101 agcctattcc ggcagctccc ggttcgcgct gagcacttcg ccggcaagat agagcgagcc
     2161 cgcgatcagc acgatctcgc gcgcgaggtc gacatcaagg ccgctcagcg cgacggggat
     2221 cgatgcggcg gctttcgcac cggggccgaa cgccgcagcg ctatgatatt cgtgccccgg
     2281 gacgggcacg acggtaaggc tggcgaggcg atcagccagc ggcgcgataa ttgcggcggg
     2341 gtccttgttc gccagcatgc cggtaatcag gtggatacgc cgcttctcgc tcccgaactg
     2401 gcgcgcgagc gcaaaacccg catcgacatt atgcgcgccg tcgagccaga tttcggtgcc
     2461 gtctggaagc agatcggcta gcggccccgc gccaagacgc tgcatgcggg ctggccagaa
     2521 ggcattcgcg acaccttgtg cgatgtcggc atcggtcggt cgcggatcag gggcgaggcg
     2581 cagcatcgcg atggcaaggc cagcgttgcg catctggtgc gcccccgcca tgcgcgggtg
     2641 cagcagcggc gcgaccgagc ccagtttcga ggtccagtgg aaagcctcgc cctcggcttc
     2701 gaccgcccag ccgtcgccct ccggaaacag cgtggcgccg acatcggcga ccttggcggc
     2761 gatggtgtcg cggatgtgcg gcggataggc gagcgtcgcg acgggcgtct cgggcttgat
     2821 gatccccgcc ttctcccacg cgatgcgcat agccggatcg tcgggcgttc cggcctcggg
     2881 cgcgagcagg aaggcctcgt ggtcgatgcc gagcgaggcg atgccggcag ccgcgggggg
     2941 cgggatgata ttcgtcgcgt cgagacggcc gccgagcccg acttcgatga tgcagtcgtc
     3001 ggcggggatg cgcgcaaagg cgaggaaagc ggcggcggtg gtgacttcga aaaagctcgc
     3061 ctgcaggtcc gctgcctcgt cgagcacctc ttcgagcagc gcggcgagca gcgcgtcgtc
     3121 gaccagcgtt ccggcaaggc ggatgcgctc gttgaaacgg acgaggtgcg ggctagtgta
     3181 ggcgtggacg cggcggccct gcgcctcgag gatcgcgcgg agataggcgc aggtcgagcc
     3241 cttgccgttg gttccggcga cgtggaaggt gcgcgggagc tggagctgcg gattgccgag
     3301 gcgcgcgcag agttcggcga tgcgttcgag cccgaggatg tcgcgccccg gtgatagggc
     3361 tgcgaggcga tcgagttggg tttgcacggc ggggtcggag ctgtgggcgt ggtcgggcat
     3421 gtcttctccc tctccccttc aggggagagg gccggggaga gggggaatct ccgataagcc
     3481 ggcctgatca tcaaaatgtg gagagagtcc ccctctccct agccctctcc cctgaagggg
     3541 ag
//
LOCUS       DATMSU010000303         6076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_100097,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000303 DATMSU010000000
VERSION     DATMSU010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100097"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..727
                     /locus_tag="VN034_08355"
     CDS             233..727
                     /locus_tag="VN034_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60649.1"
                     /translation="MPAAVDRAYRHYPKAARYHSRVEAGGAALKLYHLTAADAPVPDD
                     VGTLAARWFDEQAPAALAEDDCGFVILHRCGTDFYFLLVSVWRGSNELWEAVWYRQGD
                     MPAFAPFDAAYPPLPGVVRPTFCVWELGIVAHESAAWARYLGSPRDDAARSRWVDDIF
                     EGAV"
     gene            770..1393
                     /locus_tag="VN034_08360"
     CDS             770..1393
                     /locus_tag="VN034_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWV60650.1"
                     /translation="MQIAVLTFDGFNELDSFVAAAILNRLRGQGWEAHITAPTPRVTS
                     MNGVTIERQQPLDFAAKADAVIIGSGIKTREIAADPLMLGRIRLDPARQLIGAQCSGT
                     LLLARLGLIGDLPACTDLTTKPWVIEAGVEVLDAPFVAHGNVATAGGCLASQYLAGWI
                     IARLAGTAAAEEALHYVAPVGEKADYVTRALAAVRPFLPVETLAQTA"
     gene            1506..3521
                     /locus_tag="VN034_08365"
     CDS             1506..3521
                     /locus_tag="VN034_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWV60651.1"
                     /translation="MTAPFNFGQLKFLKALTEALFHEAEMVISADQVVANVCDLFEKI
                     GGTKLEEIQLSLTATELVLGGPFFTENDVADRVERLTDRLKNSQIDLFQDMARLRGII
                     YACYYGHWQPGLEPGDQGANAANPVHRQIGFTLPAFRQRGDYDVPLAPVHGREIDPAH
                     ILDADSVEQEYDVVVIGSGAGGGVAARNIAAQGYKVLIVEAGPFYPSHAITHHELDMI
                     ARLYKHGALQTSTNRDFVVFQGRCVGGSSTINNGICLRVNEAGRTHPDAQDVLAKWAS
                     IGAPIDAAAFHASYDAVQARLGIAPIEPRSGRHNGPHLIEGWRAYAATSNNPRDQRAV
                     ADWFAKNFGPPNTVNACAYCGYCNSGCAYGRRMGIAQTYLPEACRDFGARILPGTKAD
                     RIVWQTAYDGRREAEAVKLVLPDGSKRLVRARVGVVVAAGTIASSKLLDRSDIDRTGY
                     QVSLNVASPVVALMPDGVGGDAWDEDQMSSYVDCGDYLLESHFQPPMSMASLMPGWFG
                     DHASRMKNFGRVHSAGILFPADRRGRVRDGKLEFRLDASDDLPVLRDAMATLTKVHFA
                     AGALECYPALAKGQKVTPDMDVDAFFREAIREQDDVTLSSSHPHGGNAINEDPRHGIV
                     DLDCRVHETTNVLVTDASVFPSCIRVNAQWTTMAMAHYATARGDPFG"
     gene            3643..4371
                     /locus_tag="VN034_08370"
     CDS             3643..4371
                     /locus_tag="VN034_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HWV60652.1"
                     /translation="MTFTVAALYRFASFDDPALLRQPLIDLCVAQGIKGTLLLAREGI
                     NGTIAGTADGIAAVIDHIRALPGCAALDVKYSAAADMPFQRMKVRLKKEIVTLKVPGL
                     DPAKDAAPYVDPADWNALVDDPDTVLIDTRNAFEVGYGSFAGAVDPGTKSFGDFPGWW
                     RANADRFAGKRVAMFCTGGIRCEKSTAFLRHEGVEDVVHLKGGILAYLEQVAEADSRW
                     HGSCFVFDERVSVGHGLVEVGDKD"
     gene            complement(4514..4747)
                     /locus_tag="VN034_08375"
     CDS             complement(4514..4747)
                     /locus_tag="VN034_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60653.1"
                     /translation="MSKFALVIFLINFVLLLISIALMMPIPIWQWILLVGSGSWCIRS
                     WLAARREEEALENSYGGKNILPKKGGAPTYHDL"
     gene            complement(5021..5647)
                     /locus_tag="VN034_08380"
     CDS             complement(5021..5647)
                     /locus_tag="VN034_08380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV60654.1"
                     /translation="MSGWWERHGVPRLIKCACSQGQIMKVRSKVVPLARGHVLELGCG
                     GGINMEFYRPGQIESFSGLDPSPELLAMSHAAAAARGIDADIRSGVGEAMPFESARFD
                     TVVTTFTLCSVADQAAVLAEIRRVLKPGGTALFLEHGAAPDAGVAKWQRRIEPIWKRI
                     GGNCHLTRPIGDAYEKAGFAVDRQGAAYMPKTPRPFGWVEYGLARATG"
     gene            complement(5644..>6076)
                     /locus_tag="VN034_08385"
     CDS             complement(5644..>6076)
                     /locus_tag="VN034_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60655.1"
                     /translation="WTVSQNLLRLPRPVAAHLTGEGPGAVRDARWQDDIRFQEIVTGW
                     RPGEALQWRFAFPDPSIHRRTDRHIEPHGHHLWIDSGGYRLIPLAGGRTKIRLWTRYH
                     LATPFNAYAGWWGEFILGDIQNNILAIVAGRTGDSAGRSKS"
BASE COUNT         1047 a         1967 c         1969 g         1093 t
ORIGIN      
        1 gaggcggcgg gggcggtcgt caccgacctc cacgtctggc gcgtcggacc ggaggcgcgc
       61 gcggcgatcg tcgaagtgaa gggcgatgtg tcgtgcgaag aacttcgcga caggctgaag
      121 gatatcgtga cggtcgcgca cctgacgatc gcgctgcgtt gacccgtcga tagcggttgc
      181 gctgccacgc gatttgacta gaacataaag ggaacgaaat ggagtcccgc ctatgcccgc
      241 cgccgtcgat cgcgcctatc gccattatcc gaaagcggcg cgctaccatt cgcgcgtcga
      301 ggccggcggc gccgcgctga aactctatca tctgaccgcc gccgatgcac cggtaccgga
      361 cgatgtcgga acgctggcgg cgcggtggtt cgatgagcag gcgcccgcgg cgctggccga
      421 ggatgattgc ggcttcgtca tccttcaccg ctgcggtacc gatttctatt tcctgctcgt
      481 gtcggtgtgg cgcggctcga acgagctgtg ggaagcggtc tggtaccgcc agggcgacat
      541 gcccgccttc gcacccttcg acgcggccta tccgccgctg ccgggcgtgg tgcgcccgac
      601 cttctgcgtc tgggagctcg gcatcgtcgc gcacgaaagc gcggcgtggg cccgctatct
      661 cggctcgccg cgcgacgatg ccgcgcggtc gcgctgggtt gatgacatct tcgaaggcgc
      721 ggtataggcg cgaccccttt gatccttcat ctttcgcagg agttttgacg tgcagatcgc
      781 ggtgctgacc ttcgacggct tcaacgaact cgattcgttc gtcgccgcgg ccatattgaa
      841 ccgcctccgc ggacagggct gggaagcgca catcaccgcg ccgacgccgc gcgtcacttc
      901 gatgaacggc gtgacgatcg agcggcagca accgctcgac ttcgcagcca aggccgacgc
      961 ggtgatcatc ggaagcggca tcaagacgcg cgagatcgcc gccgatccgc tgatgctcgg
     1021 gcgcatccgg ctcgaccccg cacgccaatt gatcggcgcg caatgttcgg gaaccttgct
     1081 gctcgccagg ctcgggctga tcggcgatct ccccgcctgc accgacctca ccaccaagcc
     1141 gtgggtgatc gaggcgggcg tcgaagtgct cgacgcgccc ttcgttgcgc acggcaatgt
     1201 cgcgaccgcg ggcggctgtc tcgcatcgca atatctcgcg gggtggatca tcgcgcgcct
     1261 cgccgggacg gcggcggccg aagaagcgct ccattatgtc gcgccggtcg gcgaaaaggc
     1321 cgattatgtg acccgcgcac tggcggcggt gcggccgttc ctgccggtgg agaccctcgc
     1381 ccaaacggct tagagtcttt ccgatctagc tcggaaagac tctagctggc gatcccatac
     1441 aatacagctt gctccgcatc gctataaggc ggcattttgc ggcgcaggac aatgggggca
     1501 gggcgatgac ggcaccgttc aatttcgggc agctcaaatt tctgaaggcg ctgaccgaag
     1561 cgctgttcca cgaggccgag atggtgattt cggccgatca ggtcgtcgcc aatgtctgcg
     1621 acctgttcga aaaaatcggc ggcaccaagc tcgaagaaat ccagctctcg ctgaccgcga
     1681 ccgaactggt gctcggcggg ccatttttta ccgagaatga tgtcgcggac cgcgtggagc
     1741 ggctgaccga tcgcctcaag aacagccaga tcgacctgtt tcaggatatg gcgcggctgc
     1801 gcggcatcat ctacgcctgc tattacggcc attggcagcc ggggctcgaa cccggcgatc
     1861 agggcgccaa cgccgccaat ccggtgcacc ggcagatcgg cttcacgctt cccgccttcc
     1921 gccagcgcgg cgactatgac gtgccgctgg cgccggtcca cggacgcgaa atcgaccccg
     1981 cgcatatcct cgacgctgac agcgtcgagc aggaatatga cgttgtcgtc atcggctcgg
     2041 gcgcgggggg cggggtcgcc gcgcggaata tcgcggcgca gggttacaag gtgctgatcg
     2101 tcgaggcggg gcctttctat cccagccacg cgatcaccca tcacgaactc gacatgatcg
     2161 cgcggctcta caagcatggc gcgctgcaaa cttcgaccaa ccgcgatttc gtcgtgtttc
     2221 agggccgctg tgtgggcggc tcgtcgacga tcaacaacgg gatatgcctg cgcgtcaacg
     2281 aagcggggcg gacgcatccc gacgcgcagg acgtgctcgc caaatgggcg agcatcggcg
     2341 cgccgatcga cgccgcagcc tttcacgcca gctatgacgc ggtgcaggcg cggctcggca
     2401 tcgcaccgat cgaaccgcgc agcgggcgcc acaacggccc gcacctgatc gagggatggc
     2461 gcgcttatgc cgcgacgtcg aacaacccgc gcgaccagcg cgcggtcgcc gactggttcg
     2521 ccaagaattt cgggccgccg aacacggtca acgcctgcgc ttattgcggt tattgcaatt
     2581 cgggctgcgc ctatggccgc cgcatgggca tcgcgcaaac ctatctgccc gaggcgtgcc
     2641 gcgatttcgg cgcgcggata ttgccgggca ccaaggccga ccggatcgtc tggcagaccg
     2701 cctatgacgg ccggcgcgag gccgaggcgg tgaagctggt cctgcccgat gggtcgaagc
     2761 ggctcgtccg tgcgcgcgtc ggcgtcgtgg tcgcggcggg cacgatcgcc tcgtcgaagc
     2821 tgctcgaccg cagcgacatc gaccgcaccg gctatcaggt atcgctcaac gtcgcctcgc
     2881 cggtcgtcgc gctgatgccc gatggcgtcg gcggcgatgc gtgggacgag gatcagatgt
     2941 cgagctatgt cgattgcggc gactatctgc tcgaaagcca ttttcagccg ccgatgtcga
     3001 tggcgtcgct gatgcccggc tggttcggcg atcacgcgag ccggatgaag aatttcggcc
     3061 gcgtccattc ggcgggcata ttgttcccgg ccgaccggcg cgggcgggtg agggatggca
     3121 agctcgagtt ccggctcgac gccagcgacg acctcccggt gctgcgcgac gcgatggcga
     3181 cgctgaccaa ggtgcatttc gccgccggcg cgctcgaatg ctatcccgcg ctcgcgaagg
     3241 ggcaaaaggt cacgcccgac atggatgtcg acgccttttt ccgcgaagcg atccgcgaac
     3301 aggatgatgt gacgctgtcg agcagccatc cgcacggcgg caatgcgatc aacgaggatc
     3361 cgcgccacgg catcgtcgac ctcgattgcc gcgtccacga aacgaccaat gtgctcgtca
     3421 ccgacgccag cgtctttccc agctgcatcc gcgtcaacgc gcaatggacc acgatggcga
     3481 tggcgcatta tgcgaccgcg cggggcgatc cgttcgggtg acggcttttt ccccatcccg
     3541 ctcgtgctga gtgtgtcgaa acaccgtcct tctttctgac ctttgcgcga aaaaagaacg
     3601 gcccttcgac aagctcaggg cgaacggcta tggggcggcg gaatgacctt caccgttgcc
     3661 gccctctatc gcttcgcgtc gttcgacgat cccgccctgc tgcgccagcc gctgatcgat
     3721 ctttgcgtag cacagggcat caagggcact ctgttgctcg cgcgcgaagg gatcaacggc
     3781 acgatcgcgg gaaccgcgga tggcatcgcc gccgtcatcg accatatccg cgcgcttccc
     3841 ggttgcgctg cgctcgacgt caaatattcg gccgccgccg acatgccgtt ccagcggatg
     3901 aaggtgcggc tgaagaagga aatcgtcacg ctcaaggtcc ccgggctcga ccccgcgaag
     3961 gacgccgcgc cctatgtcga tcccgccgac tggaacgcgc tcgtcgacga tccggacacg
     4021 gtgctcatcg acacgcgcaa cgcgttcgag gtcggctatg gcagcttcgc gggcgcggtc
     4081 gatcccggaa cgaaaagctt cggcgacttc cccggctggt ggcgcgccaa tgccgatcgt
     4141 ttcgcgggca agcgcgtcgc gatgttctgc accggcggca ttcgctgcga aaaatcgacc
     4201 gcctttttgc gtcatgaagg cgtcgaggac gtcgtccacc tgaagggcgg catcctcgcc
     4261 tatctcgaac aggtggccga ggccgacagc cgctggcacg gcagctgctt cgtcttcgac
     4321 gagcgcgtga gtgtcgggca tggattggtc gaggtcggtg acaaggatta agacaggaac
     4381 ggttggtcca gcggtgtaag cggctttcgg ccgattgctg cgtggcccgg ctccttatgg
     4441 gcctgctgac gctacgggta ttttggcaag ggatggggga gcaggcgatt ggcccggccc
     4501 ccccgttcac gcttcaaagg tcgtggtagg ttggagcgcc gccttttttt ggcaatatgt
     4561 tcttgccgcc atagctgttc tcaagtgctt cttcttctct ccgcgccgcg agccatgacc
     4621 gaatgcacca gctgccggag ccgacgagaa ggatccactg ccagatcggg attggcatca
     4681 tcagggcaat agatatgagc aacagaacaa agtttataag gaatataacc aacgcgaact
     4741 tcgacattgc ttcgtccttt cgaaataacg aaaggatgat gcacgtatgg agttagaaaa
     4801 tcctaaattt gttgttgcat ttgtccattg ccgtatcgcg ccgttcccat caaagaccaa
     4861 ggccggcggc atgacgaaat aggatcgctc gcgcggtgaa gttctttcct atatttgacc
     4921 cctatggttc tcgttcgcgg ttttgactca ccgaaaagac gagcgcggat ccgcgccacg
     4981 gcctgaaccg acacccgcgc gcaaccttca cgcccgatcg tcacccggtc gcgcgcgcca
     5041 agccatattc gacccagccg aacgggcggg gcgttttcgg catataggcg gcgccctgcc
     5101 ggtcgacggc aaatcccgcc ttttcatagg cgtcgccgat cgggcgggtc aggtggcagt
     5161 tgccgccgat gcgcttccag atcggttcga tccgccgctg ccatttcgcg acccccgcgt
     5221 cgggcgcggc gccatgctcg aggaacagcg ccgtgccgcc gggcttcagc acgcggcgga
     5281 tttcggcgag caccgccgcc tgatcggcga ccgaacagag ggtgaaggtc gtgaccaccg
     5341 tgtcgaaccg cgcgctttcg aacggcatcg cttcaccgac gccgctccgg atgtcggcgt
     5401 cgatcccgcg cgccgccgcc gcagcgtggc tcatcgccag cagttcgggc gaggggtcga
     5461 ggcccgaaaa gctctcgatc tgcccgggcc ggtagaattc catgttgatc ccgccgccgc
     5521 agccgagttc gagcacatga ccgcgcgcga gcggtacgac cttgcttctg accttcatga
     5581 tctgcccctg cgaacaggcg catttgatca ggcgcgggac gccgtgcctc tcccaccaac
     5641 cgctcatgac ttgctccttc cggccgagtc gccggtccgc ccggcgacga tggcgagtat
     5701 attgttctgg atgtcgccga ggataaactc gccccaccag ccggcatagg cgttgaacgg
     5761 cgtcgcgaga tgatagcgcg tccacagcct gatcttggtt cgcccgccgg cgagcgggat
     5821 caggcggtag ccgccgctgt cgatccacaa atggtggccg tgcggttcga tatgccggtc
     5881 ggtgcgccgg tggatcgacg gatcggggaa ggcgaagcgc cattgcaggg cctcgcccgg
     5941 ccgccagccg gtgacgattt cctggaaccg gatatcgtcc tgccagcgcg cgtcgcggac
     6001 cgcgccgggg ccttcgccgg tcagatgcgc cgcgacgggg cgcggcaggc gcagcaggtt
     6061 ctggctgacg gtccag
//
LOCUS       DATMSU010000304         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_128691,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000304 DATMSU010000000
VERSION     DATMSU010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128691"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VN034_08390"
     CDS             complement(<1..625)
                     /locus_tag="VN034_08390"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWV60656.1"
                     /translation="MKLISGNSNLPLARAIADYLELPLTDTSVRRFADEEVFVEIHEN
                     VRGQDVFVVQPTNFPANDNLMELLIINDALRRASAKRITAVVPYFGYARQDRKPGPRT
                     PISAKLVANLITTSGADRVLAIDLHAGQIQGFFDIPTDNLYAAPVMSADIQARFGGKN
                     LMVVSPDVGGVVRARALAKRLDNAPLAIVDKRRERAGESEVMNIIGDV"
     gene            781..2442
                     /locus_tag="VN034_08395"
     CDS             781..2442
                     /locus_tag="VN034_08395"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWV60657.1"
                     /translation="MIQELSIVLSQMTQVVGNLAANADAMRSVRARHPAADLILYPEL
                     QLIGYPPEDLVLKPALAERAAKLLVELAAETADGGPAMLVGSVEREGDSLYNIVALLD
                     GGKIVATRRKHELPNYGTFDEKRVFAPGPLPDIVEWRGVKLGLPICEDGWLPTVCAHL
                     AAHGAELLISVNGSPYEIDKDERRLTQVFRSRVAETKLPVIFLNRVGGQDEIVFDGCS
                     FILNDDGATAHRLTDWEAEERVTRWTNKGDGRWTCEPGAIAEWEAHPADIYSAMIVSL
                     RDYVERNRFPGVVLGLSGGIDSAICAAIAADALGPDKVWCVMLPSRFTSEESLDDAAE
                     CARMIGCRLDTIPIVPAVEAFDAMLAGSFADAQVDITEENVQSRIRGVTLMALSNKFG
                     PMLLTTGNKSEMSVGYATIYGDMAGGYNPLKDAYKMTVFAVAKWRNENVPRLSRNPVT
                     PVMPDTIITKPPSAELRPDQKDEDSLPPYADLDRMLHMLVEEEASVDDVVARYGFDRE
                     AVTRIERLLMLAEYKRRQAPPGVKLSTRNFGRDRRYPITHGFRTG"
     gene            2459..2875
                     /locus_tag="VN034_08400"
     CDS             2459..2875
                     /locus_tag="VN034_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV60658.1"
                     /translation="MGLLGPKAPPERAGQFIELLRITVAVLILIHGVYRLAAGLVAPF
                     GTWLDSLGFPYGYGWAMAVTLYELVGPALMLTRRWTSFAALGHAGILTLGMILVHLPA
                     GWFVVGGGRNGMEYSVILIVALLGIAWAWWPARRSA"
     gene            2914..4224
                     /gene="gltX"
                     /locus_tag="VN034_08405"
     CDS             2914..4224
                     /gene="gltX"
                     /locus_tag="VN034_08405"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWV60659.1"
                     /translation="MTVTTRFAPSPTGSLHVGNVRTALHNWLWARRHGGRFLLRIDDT
                     DLERSKEEYVEGIRADLAWLGLDVDGEERQSARFALYEGEFEKLKAAGRVYACYETPE
                     ELDIRRKVLLSRGLPPVYERKPEDAPVPEGVAPHWRFRLDHEAPIEWTDMVRGPQHFE
                     PKTMSDPVVRRADGSWLYLLPSVIDDIAMGITHVVRGEDHVSNTAAQIQMFAALGAKP
                     PAFAHEALLVGTEGKLSKRLGSLGMASLQGDGIEPVALVALLARLGTSDPVEPVTTPA
                     PLIESIDFARFGRAPARFDEGELALLNQKILHHTDHAAVDARLPAAITEARWNAIRPN
                     LMTVAEATDWVAVFDGPFAPPPADAADRPVLEAAAKAAPSVDWSADPWHSLTGAVKEM
                     TGAKGRALFLPLRRALTGRDHGPDMAELLPLIDKDEAVARLSAA"
     gene            4379..>4708
                     /locus_tag="VN034_08410"
     CDS             4379..>4708
                     /locus_tag="VN034_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWV60660.1"
                     /translation="MTLIPLISAIVAAPEATTAPAGPALQPRNSYGAGNRHRPVAALF
                     AIGLPAALVAAVALSPMIIVDKPTTATPPWKSIILPKPKPPEPKPDPQPRTQPQPQFT
                     APPSPLPP"
BASE COUNT          792 a         1540 c         1600 g          776 t
ORIGIN      
        1 tcacgtcgcc gatgatgttc atcacttcgg attcgccggc gcgctcgcgg cgcttgtcga
       61 cgatcgcgag cggcgcgttg tcgagccgct tggcgagcgc gcgcgcgcgc acgacgccgc
      121 cgacgtcggg cgaaacgacc atcagattct tgccgccgaa gcgcgcctga atgtcggcgc
      181 tcatcaccgg cgcggcatag agattgtcgg tcgggatatc gaaaaagccc tggatctgcc
      241 cggcgtgcag gtcgatcgcg agcacgcggt cggcgcccga ggtggtgatc agattcgcga
      301 cgagctttgc cgagatcggc gtgcgcgggc cgggtttgcg atcctggcgc gcatagccga
      361 aataggggac gaccgcggtg atgcgcttcg ccgacgcgcg gcgcagcgcg tcgttgatga
      421 tgagcaattc catcagattg tcgttcgcgg ggaaattcgt cggctggacg acgaacacgt
      481 cctgcccgcg gacattctcg tggatttcga cgaaaacctc ctcgtcggcg aagcggcgca
      541 cgctggtgtc ggtgagcggc agttcgagat agtccgcgat cgcgcgggcc agcggcaggt
      601 tgctgttgcc ggagatgagt ttcatggggt gcgaagccct ttcgcgcggg gtgggaatgc
      661 gcgccccctt agccagcgca ggcgcgaagg ggaagggtca tcttgccatt gcgcgaccaa
      721 aggaaggaag cggggcggaa tattgtttca gtgcaatggc accgcggcta aacgaacggc
      781 atgatccaag agctctccat cgtcctttcg caaatgacgc aggtcgtcgg caacctcgcc
      841 gccaacgccg acgcgatgcg cagcgttcgg gcgcggcatc cggcggccga cctgatcctc
      901 tatcccgagc tgcagctgat cggttatccg cccgaggatc tggtgctcaa gcccgcgctg
      961 gcggagcgcg cggcgaagct gctcgtcgaa ctcgccgccg agaccgccga cggtgggccg
     1021 gcgatgctcg tcgggtctgt cgagcgggag ggcgacagcc tctacaatat cgtcgcgctg
     1081 ctcgacggcg gcaagatcgt cgcgacgcgg cgcaagcacg aactccccaa ttacggcacc
     1141 ttcgacgaaa agcgcgtctt cgcccccggc ccgctccccg acatcgtcga atggcgcggg
     1201 gtcaagctcg ggctgccgat ctgcgaggat ggctggctgc cgacggtgtg cgcgcatctc
     1261 gcggcgcatg gcgccgaact gctgatctcg gtcaacggca gcccctatga aatcgacaag
     1321 gacgagcggc ggctgacgca ggttttccga agccgtgtcg ccgaaacgaa gctgccggtc
     1381 atcttcctca accgcgtcgg cggacaggat gaaatcgtgt tcgacggctg ctccttcatt
     1441 ctcaatgatg atggcgcgac cgcgcaccgg ctgaccgact gggaagccga agagcgcgtc
     1501 acgcgctgga ccaataaggg cgacgggcgc tggacctgcg agcccggcgc gatcgccgaa
     1561 tgggaagcgc accccgccga tatttacagc gcgatgatcg tcagcttgcg cgactatgtc
     1621 gaacgcaatc gcttccccgg ggtcgtgctc ggcctgtcgg gcggaatcga ttcggcgatc
     1681 tgcgccgcga tcgccgccga cgcgctgggg ccggacaagg tctggtgcgt gatgctgccg
     1741 agccgcttca cgagcgagga gagccttgat gacgccgccg aatgcgcgcg catgatcggg
     1801 tgccggctcg acacgatccc gatcgtcccc gcggtcgagg cgttcgatgc gatgcttgcg
     1861 ggcagcttcg ccgacgcgca agtcgacatc accgaggaga atgtccagtc gcgcatccgc
     1921 ggcgtcacct tgatggcgct cagcaacaaa ttcgggccga tgctgctgac gacgggcaac
     1981 aagagcgaga tgagcgtcgg ctatgcgacc atctatggcg acatggcggg cggctataat
     2041 ccgctgaagg atgcgtacaa gatgacggtg tttgccgtcg cgaaatggcg caacgaaaat
     2101 gtgccgcgcc tgtcgcgcaa tccggtgacc ccggtgatgc ccgatacgat catcaccaag
     2161 ccgccgagcg cagagctgcg ccccgaccag aaggacgagg acagccttcc tccttacgcc
     2221 gacctcgacc gtatgctcca catgctagtc gaggaggaag cgagcgtcga cgatgtcgtc
     2281 gcgcgctatg gcttcgatcg cgaggcggtg acgcggatcg agcggctgtt gatgctcgcc
     2341 gaatataagc ggcgccaggc gccgccgggg gtcaaattgt cgacgcgcaa tttcgggcgc
     2401 gaccggcgct atccgatcac gcacgggttt cggacgggat aatcaggggg gaagcacgat
     2461 gggattgctg ggaccgaagg cgccgcccga acgcgcgggg cagtttatcg agctgctgcg
     2521 gatcaccgtt gcggtcctga tcctgatcca cggcgtctat cgcctcgccg cggggctcgt
     2581 agcgcctttc ggcacttggc tcgacagcct cggctttccc tatggctatg gctgggcgat
     2641 ggcggtgacg ctgtatgagc tggtcggccc ggcgctgatg ctgacgcgcc gctggaccag
     2701 cttcgccgcg ctcggccacg cgggcattct gacgctcggc atgatcctcg tccaccttcc
     2761 cgcgggctgg ttcgtcgtcg gcggcgggcg caacgggatg gaatatagcg tcatcctgat
     2821 cgtcgcgctg ctcggcatcg cctgggcctg gtggcccgcg cggcgcagcg catagctatc
     2881 gcctcgcggg caaatgcggc tataggcgcg cggatgaccg tcaccacccg tttcgccccc
     2941 tcacccaccg gcagcctgca cgtcggcaat gtccgcaccg cgctccataa ctggctgtgg
     3001 gcgcggaggc acggcgggcg attcctgctg cgcatcgacg ataccgacct cgagcggtcg
     3061 aaggaggaat atgtcgaggg catccgcgcc gacctcgcgt ggctcgggct cgatgtggac
     3121 ggcgaggagc ggcagtcggc gcgcttcgcg ctttacgagg gtgagttcga gaaactgaag
     3181 gcggccggac gtgtctatgc ctgttacgag acgcccgagg agctcgatat ccggcgcaag
     3241 gtgctgctct cgcgcgggct gcccccggtt tatgagcgca agcccgagga cgcgccggtg
     3301 cccgaaggcg tggcgccgca ctggcgcttc cggctcgacc atgaggcgcc gatcgaatgg
     3361 accgacatgg tccgcggccc gcagcatttc gagccgaaga ctatgtccga ccccgtcgtg
     3421 cggcgcgctg acggcagctg gctctatctg ctgccgagcg tgatcgacga tatcgcgatg
     3481 gggatcacgc atgtcgtgcg cggcgaggat catgtgtcga acaccgcggc gcagatccag
     3541 atgttcgcgg cgctgggggc gaagccgccc gccttcgcgc acgaagcgct gctcgtcggc
     3601 accgagggca aattgtcgaa gcggctcggt tcgctcggca tggcgagcct gcagggcgac
     3661 ggaatcgaac cggtcgcgct cgtcgcgctg ctcgcgcggc tcggcaccag cgacccggtc
     3721 gagccggtga cgacgcccgc accgctgatc gagagcatcg atttcgcgcg cttcggccgc
     3781 gcccccgcgc gcttcgacga gggcgagctg gcgttgctca accagaagat tttacatcac
     3841 acggaccatg ccgcagtcga cgctcgcctg cccgccgcga ttaccgaagc gcgctggaac
     3901 gcgattcgcc cgaatttgat gacggttgcc gaggcgaccg actgggtggc ggtgttcgac
     3961 ggcccctttg cgccgccgcc tgccgacgcc gccgaccggc cggtgctcga agcggcagcg
     4021 aaagcggcgc cctcggtcga ttggtcggcg gacccctggc attcgctcac cggcgcggtg
     4081 aaggagatga ccggcgctaa gggccgcgcg ctcttcctgc cgctgcgccg cgcgctgacg
     4141 gggcgcgatc acggtcccga catggccgag ctgctgccgc tgatcgacaa ggacgaagcg
     4201 gtcgcgcggc tttcggccgc ctgatccaag ccgcttcagc ccgccactcc aacgatcgat
     4261 gcttgcttac cgatccgctt gacaagttta gtgatgttat aatatcacgt gactctgacc
     4321 tggccggttc gacggtcaga acgccgcggg gccgggtcga caggcaagga gagatgccat
     4381 gaccttgata cccctgattt cggcgatcgt cgccgcgccc gaggcgacca ccgcgccagc
     4441 cgggccggcg cttcaaccgc gcaacagcta tggcgcgggc aaccgccacc ggcccgtcgc
     4501 cgcgctgttc gcgataggac tacccgcagc actggtcgcc gcggtggcgc tgtcgccgat
     4561 gatcatcgtg gacaaaccca cgaccgccac gccgccctgg aaaagcatca ttctgcccaa
     4621 gccgaagccg cccgagccga agcccgaccc ccagccacgg acgcagccgc agccacagtt
     4681 tacggcgccg ccgagtccgt tgccgccg
//
LOCUS       DATMSU010000305        22890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_5738, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000305 DATMSU010000000
VERSION     DATMSU010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 22890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22890
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5738"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VN034_08415"
     CDS             <1..255
                     /locus_tag="VN034_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HWV60661.1"
                     /translation="IEVIAEVPDGPPQRFRWRRAFHAIRRYEGPERIAAEWWRRRDNC
                     GLTRDYYRVEDAQGRRFWLFRHGLYDEKPDPRWYIHGVFA"
     gene            252..3521
                     /locus_tag="VN034_08420"
     CDS             252..3521
                     /locus_tag="VN034_08420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HWV60662.1"
                     /translation="MSEETANPGFAELVAATNYSFLRGASHPAEMVAEAIALGMSGIG
                     IADRNSVAGVVRAWAFLKDLQVKKPEVVEGFRLVVGARLVFADGTPDIVAYPVNREGW
                     GRLTRLLSTGNLRAQKGDCILELGDLLDHCDDLFLIAMEGDEELLRTLKRKRPKSVWL
                     AATMSQSGADARRLAELQRLSAATGVPLLATNDALYATKAQRPLHDIITCIREGTTVS
                     KAGKLLRANGERYLKAPAEMKRLFDGYSKAIEASAKLLARIGFGLEDLQYEYPHEPVP
                     PGWKPFPYLHHLVKTAAEERYKTPLPPKVRKLLADELRLIKKRRYVYYFLTVYDLVRH
                     ARAQKPPILCQGRGSAANSIVCFLLGVTSVDPMKHDLLFSRFVSAERDEPPDIDVDFE
                     HERREEVIQYIYDRYTRDRAAIAATVIHYRPRSTIREVGKALGLSEDVTARLADTSWG
                     SWGDEVPVERLVEAGLNPNNGEIERLHRFVGELLKAPRHLSQHVGGFVLTEGRLDELV
                     PIHNAAMEDRTFIEWDKDDIDALGLMKVDVLALGMLTCIRKCYDLMREHGLGNHTLEL
                     DIDCEDKAVYKMLQRGDSIGVFQVESRAQINMLPRLKPKVFYDLVVQVAIVRPGPIEG
                     DMVHPYLKRRNGEEEVKFPSPAAPHDPNELHDVLDKTYGVPLFQEQAMKLAIVAAGFT
                     AEEANGLRRAMATFRNVGTIDNFREKMVGGMVARGYEPDFAERCFKQIEGFGSYGFPE
                     SHAQSFARLVYVSSWIKHYHPAVFACGILNSQPMGFYAPAQLVRDAQGHGVEVREVDV
                     NASGWDNSLEPKADGSLALRLGFRQVDGFREEWATQIADARATPFASVEDLARRANLP
                     SRALRLLAEADACRSMGLDRRPALWDARRVRQGVLPLFGAAETDELGVEEDARLPPTP
                     LVEHVLTDYQTTRLSLKGHPMAFLRRDFEREGVLSAAGVAAAKNGSIIRTAGVVLIRQ
                     RPGRGNAIFITLEDEGGIVNILLWARHFERQRRAVMASRLMLAEGEVQRSKEGVIHLM
                     ATRIVDRTAMLDTLGSDRRFDPDVCRADEVRHPQLPRGHAAQHGHPRNVRILPKSRDF
                     H"
     gene            complement(3723..4967)
                     /locus_tag="VN034_08425"
     CDS             complement(3723..4967)
                     /locus_tag="VN034_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HWV60663.1"
                     /translation="MHKPAQSPQRSLPGDREMVVMMAMVMALNALAIDSMLPALPAIG
                     EALGVTVANDRQHVISIYLLGIGFGSLLYGPLSDRFGRKGVLVPALFAYLALSIGCGL
                     ATSFAMLLALRFVHGLVSAALGVIVVAVIRDLFSGDAMAKRLSLIFLVFMIVPIIAPT
                     IGAGIAAVAGWRAIFIVLAVMSIVMLFWLRRLPETLDPADVRPLDFKTMVAGWATVTR
                     HRRAAGYMIASGMMQGALYGYLNSSEQIIAEVFGARAMFPLIFAIVAVGIAIANFSNA
                     AIVERFGARRVSQSAVFAFMATSILQIVAALSGAETLWMFTALMMVNVGLVGFIGSNF
                     GSIAMEDFGHMAGVASSYQSFAKTLLAATVGATIGQQYDGSTLPLAYAFLISAVVGLA
                     LVFWAERGKLFTRPGTAPKSPY"
     gene            complement(5101..5403)
                     /locus_tag="VN034_08430"
     CDS             complement(5101..5403)
                     /locus_tag="VN034_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60664.1"
                     /translation="MSQVDGSYDCVTKSPMGDQKSVFTVASNGDSFTGQNAGAMGSLD
                     VENGKVDGNKLSWTMNMKVPMPMTLECEATIDGDALTGTIKAGAFGSMAMTGTRQG"
     gene            complement(5430..8321)
                     /locus_tag="VN034_08435"
     CDS             complement(5430..8321)
                     /locus_tag="VN034_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWV60665.1"
                     /translation="MARFHRFAVALALSTSLVATAPAFARSAAKTAAPAPTADLVKAV
                     DIPYEAFTLDNGLRVIVHEDRKAPVVAVSVWYRVGSKHEPAGKTGFAHLFEHLMFNGS
                     ENAPNDFFEPLRQVGATDFNGTTFLDRTNYFETVPTGALDLALFLESDRMGHLLGAVT
                     QEKLDNQRGVVQNEKRQGDNNPYGLLRYEIFENLFPKGHPYHHSTIGSMADLDAASLD
                     DVKKWFADNYGPNNAVLVLAGDIDVPTAKAKVGEWFGDIPRGPEIKAPATSLPTLPAP
                     LAKEVKDLIPTTRIYRMWTMPGLNDAESVPLQMAMSVLGGLSSSRLDNALVRKDPIAV
                     SVSAGASPFEDAGIVLVQADVKAGVDPAHVAKRLDEEIAAFLASGPTADELQRATASY
                     LGGAISQLESVGGFGGKAVTLAEGALYSNNPAYYKVELERLAKATPEQVKAAANKWLS
                     RPAFSLTYTPGERTEGGEHRGGAVVAAKGAPAVAPDRYWNPALGDVGPDSGIGAATAI
                     ADRSQFPSVTGLKALEFPEIERAKLKNGIEVVFARRTTVPTVNVQVSFDAGYAADPRS
                     ALGTQSLMLSLMDEGTTSLDSIAFAEAKERLGAQTYGYADADETALGLFALKPNLRAS
                     LALLADYVRNPAFDAKELERVRAQQLNRLKAELNEPRAIASRVLMPALYGAEHPYGIP
                     PSGLGNAEAVTAATRDQLAAFHSAWIRPDNARIFVVGDTTLAEVKKELDTAFGDWKAP
                     ATPKPTKHFEVAVPKPQPRILLVDRPKSPQSVILAGKVLDAKGGDGLEVLRAANDIFG
                     GNFLSRFNMNLRETKGWSYGVRTSVSGETNRVSWIASAPVQADRTGDSIKELQSDLKA
                     FLGDKGVTKEELQRTVNGSVRELPGSFETSSDVLGGLRAIVKFDRPDDYYEKLPATYE
                     AMTPEALDAAARKALSTDDLVYVVVGDAAVVKPQLDGLGLPVETVSPAN"
     gene            complement(8418..10550)
                     /locus_tag="VN034_08440"
     CDS             complement(8418..10550)
                     /locus_tag="VN034_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylase"
                     /protein_id="HWV60666.1"
                     /translation="MLALIVVAAVSLAFWEPLTAEAPPAPAFKPADVRIARDNFGVPH
                     IFGKTDADVAYGVAYAHAEDDFSTLQEVLAMTRGRAGAMLGPDGAKIDYAAALLDIRA
                     TTRRDWPRLPDDVKKLFTAYAAGLNHYADKHPDEVRLSGLFPVTGEDVVAGFVLRSPF
                     FFGLDSVLGSVTEGTEIGREGGPALDKSGKLVPRELTPVGTDPADNGSNAMAVAPARS
                     TDGATRLVSNSHQPWTGGVAWYELVVHSEEDWDFAGANFPGSPYPFLGHNKYLGWTNT
                     VNRPDLIDVYKLVLDDSGKNYRFDGAWRPLEEKRIWLKVRFGPFVLPVPRTIWRSVHG
                     PVVKNEKGAFAIRYAGIDQANMVTQYYRLNKAKTFAEWRAAMAGQGVPATNFIYADAK
                     GNIGLFYNAMFPDRPAGFNWRGVLPGDTSADLWTKTLPFDRVPALVNPRSGYVMNANN
                     TPWVAAGPGDELDAAAFSPLLGIEDDMTNRATRLIELFEASGQIDEARLKAIKYDTAY
                     SRSGYAKAWMDRLLALDTKGDPALAEAQRLLRKWDWTLDGRGKGDALALMLLRPANGR
                     HYQRRAAPDPRTVLKETVAHLQEHFNGLDPKLGTVLRLRHGEGANRVDLPLDGGNDTV
                     RASTLWDEEPDGRLKVRHGDSFIMFVTWDRNGRVRSESIQPFGAATTRPDSPHYNDQA
                     PLFVAHKLKPVLFDPAALKASGARFYRP"
     gene            10773..13355
                     /gene="clpB"
                     /locus_tag="VN034_08445"
     CDS             10773..13355
                     /gene="clpB"
                     /locus_tag="VN034_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWV60667.1"
                     /translation="MNLEKFTDRAKGFLQAAQTIAIRMNHQRIAPEHIAKALLEDNQG
                     MAAGLIANSGGDTARAVAGIDALLAKLPAVSGSGAQATPGLDNDAVRLLDQAEQVAAK
                     AGSEFVAVQNILLAMVLAPSTPVGKAFADAGVKPEALNAAIAKLTGGRTADSASAEDR
                     YDALQKFARDLTEVAREGKLDPVIGRDEEIRRTVQILARRTKNNPVLIGEPGVGKTAI
                     AEGLALRIVNGDVPDSLKDRRLLALDMGALIAGAKYRGEFEERLKGVLDDVKAAEGEI
                     ILFIDEMHTLVGAGKGEGAMDASNLLKPALARGELHCIGATTLDEYRKHVEKDPALQR
                     RFQPVFVGEPTVEDSISILRGIKEKYELHHGVRITDGAIVAAATLSNRYISDRFLPDK
                     AIDLMDEAASRIRMEVESKPEAIESLDRRIIQMKIEESALGKESDAASKDRLASLQAE
                     LANLEQQSAELTQKWQAEKDKIHAEAKIKEELDAARGQLDQAQRAGDLAKAGELSYGT
                     IPGLEKRLAEAEAAAGNAMLREEVTADDIAAVVSKWTGIPVDRMMEGEREKLLGMEDT
                     LGQRVIGQDEAVRAVSTAVRRARAGLQDPNRPLGSFLFLGPTGVGKTELTKALARFLF
                     DDDNAMVRIDMSEFMEKHSVARLVGAPPGYVGYEEGGTLTEAVRRRPYQVVLFDEVEK
                     AHPDVFNILLQVLDDGRLTDGQGRTVDFTNTLIILTSNLGSQAIAALPDDAPVEQAEP
                     AVMEVVRAHFRPEFLNRLDEIVLFNRLAQQHMGGIVDIQVARVQKLLADRKVTLDLTD
                     AARAWLGRVGYDPVYGARPLKRAVQKYLQDPLADLILKGEVKDGQTIRVDEGDGALVL
                     TPVG"
     gene            complement(13388..13627)
                     /locus_tag="VN034_08450"
     CDS             complement(13388..13627)
                     /locus_tag="VN034_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60668.1"
                     /translation="MAHFLLAFLLLGSGADTPPAAEPAKPADPVICKTYDVTGSLVRK
                     KKVCRTKSEWSKASSELNETLNRFVEDRRTRPAGN"
     gene            complement(13684..14973)
                     /locus_tag="VN034_08455"
     CDS             complement(13684..14973)
                     /locus_tag="VN034_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HWV60669.1"
                     /translation="MSHSDVRDLLTAAAQARNAGDTRRAIDHLQAALSRAPGDPFVLN
                     SLGMIALDARQLADARDYFERAIENDAAAPELWMNLAKVHRVAEADRLEAESLDGALA
                     IDQRHFMANVRRAELHEKRGETAAAMHRWSGVIALAEASGTANASLADLLEHARRYIA
                     GVASGFENSLSPRLAAFRRNGVEPLAMRRFETCVDTILGRRRIYANECHGLHYPFLPA
                     DEFFDRSHFPWIEQLEAETEHIRAELLAMLATDTEGLEPYVRQEPGTAPNKWTTLDNS
                     PDWSVHFLWKHGVRQDEACARMPRTAAVLDQLPLSDIPGRSPTAFISLLRPGAHIPPH
                     TGVTNVRAIVHLPLIVPERCAFRVGGETRDWVEGQAFVFDDTIEHEAWNRSDALRAIL
                     IVDVWNPHLSESEREMLRGLFQEMVDYGLDAGGSVDD"
     gene            complement(15055..18030)
                     /locus_tag="VN034_08460"
     CDS             complement(15055..18030)
                     /locus_tag="VN034_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60670.1"
                     /translation="MKKTHYSKLKLGAAPFVMSAALLSAPAYAQDAADEGATDVTSEI
                     VVTGTLVRNPNLTTSSPVNVTSADEIELRQVNVAEDILRDVPGVAANVGSSVNNGNGG
                     ASFVDLRGLGPSRNIVLLDSQRIAPANLNGNVDLNNIPLALIERVDVLTGGASTTYGA
                     DAVSGVVNFITKRDFAGVDLATSYQITERGDGRIFRADLTVGANFDDGRGNAVLSVGY
                     QKADPVFFGGERPGSDLTIDSFDGSGSGASGTASPSRFSIPGVGNLQIDPATGNLVPQ
                     FALFNFNPYNIFQTPFERYNMFSSARYEVSDSVEVFARGMFSQNTVQTIIAPSGAFSD
                     PVFIPLSNPFMTAGARNTFCANNDFDPDTDGIQTLTGAQCAAAATATDPNDPNYQEVS
                     TNLGRRLVEAGGRVSSYKTQLFDFMAGVRGSITDTLRYEVSGNYGESENTQTILNYAS
                     LTRLQQALRAPDAANCQNPAGGCVPVNVFGPSGSITPEMLDYLLVPATSGNRTSLAQV
                     RGLLSGELGSFSESSDAISFAVGAEYRKYTAEVFADQLAQSPGELGGAGGATTPVKGG
                     YNVREVFGEIIAPLFAGLTLEAGARYSKYEVDDATDQSYDTWTYKVGGTWEAFDGFKL
                     RGNYQRAVRAPNIGEIFTPNAVGLVNLKRDPCQSDGTTIDATSNLGQVCVAQGALVNG
                     AGNIAAFDPPSAGQINASFDYSAPLKPEKADTFTVGAIFQPSFIPNLTLSVDYYNIII
                     NDAISFPTVGDVLSDCFANLTADSATSVACTSIQRDPAIGGLDGSAATVPGVPLALTN
                     SGRLATDGIDVAANWKTDLTDDIALSLGFTGNYTFHSKFRSKPGGLNRECVGYYSINC
                     TPTGSDLSGGSILPKFSFNQRTTLSFGDIDVSLLWRHISSTKQEPFDADETQFGTNGP
                     AFEPFRKIKAFNWFDLTTRASVTENFELTLSVMNLFDKDPPTVGSNIGSTTYNSGNTY
                     PSTYDALGRRYAVGARLKF"
     gene            18206..18343
                     /locus_tag="VN034_08465"
     CDS             18206..18343
                     /locus_tag="VN034_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60671.1"
                     /translation="MPNGTKYNGMQAGIGDILYYGRTFFSEVQRPGLTMRRALIMSHM
                     G"
     gene            18372..18914
                     /locus_tag="VN034_08470"
     CDS             18372..18914
                     /locus_tag="VN034_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60672.1"
                     /translation="MTPEGVLSMKNSVLWASGLVAAALGAIAVPDAHAQDMSGDILRC
                     GAIADNPARLACFDGLLPKARQNHADAGAKSEADFGLTEVQRNEKIKRDAPEPLAGRE
                     RAPATEPRVEATIAAIDMDRFRAVFTLDNGQVWQTTSFGTLNTVPRAGQKVVVQTGPL
                     GGYRLTLEGKKNEIGVKRVK"
     gene            complement(19305..19550)
                     /locus_tag="VN034_08475"
     CDS             complement(19305..19550)
                     /locus_tag="VN034_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60673.1"
                     /translation="MYRSLLLLASVLSLSLPGFAVASEDKTEADPSTVRCRSIKEVGS
                     RIPKRVCKTNEEWAREREAARQAMDDRNRSSHCSNGC"
     gene            complement(19626..22634)
                     /locus_tag="VN034_08480"
     CDS             complement(19626..22634)
                     /locus_tag="VN034_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60674.1"
                     /translation="MKKTSFTALRAAAAPAALCVALLSTASFAQEAPAEDASAEDTII
                     VTGSRIASPQAESNAPLQVIGSEAITQSGVTNVQELLLKNPAFGTPTLSRTNSAFLTS
                     GTGVATIDLRDLGSNRTLVLVNGRRVVSSLSGSSTVDLNVIPTQFIERVDVLTGGASS
                     LYGSDAVAGVVNFVYKKDFQGLEGNAQYGVTERGDDIRYQANLTMGANFDDGRGNIMV
                     HLGYSNEKGLLSRQRKNTFTDDSDTAALTGDLADYGVSSGPFYSGFSEQGSFTSGDYT
                     FTYSPTGVLQPCFTANGPTCSSDLGTGVGPNGFNRQYYRTLAVPVKRYTVAAVGHYDV
                     TDNISMFFEGTYNKTQSSRIIEPFALASDGSTGVYPSSGVMPIENYIIAPDGTTSIAI
                     NPIVPGAIAAQAVDSDGDGLRDIGFARRLTEFGTRNGQTTRDFLRFVVGFEGTLGEKF
                     NWDLSYNYGQVTENQVSNGQVNVVNFRNALAAMTDVDDVNGNGLTTDVVCVNAEARAN
                     GCVPINIFGKGAITPEAIEYIAAQQSLQTDIKQQVVQGNISGSLFELPAGPLGVALGF
                     EYRKEESVEDNDALTNQGLNAGNKLPDTSGRFDVAEAYAEIKVPILRDTPFFQLLEVG
                     GAFRVADYSTVGTIYSYNVQGTWEPIDDLRLRATWARAVRAPNIGELFSGLSQTFPSG
                     LQDPCVGIGATGGGALGDNCRADAGVAANIAANGTFTLTQPDIQGISGFNGGNPNLNE
                     EKADSWTVGAVLNPRSIDALRNLTLTVDYYNIKIENIIATVGRGTILDQCYNQGNDPL
                     CDLIIRRPTDTATNSAGSIEFINDLAVNAARLETQGLDVTVNWFTPVNFFDTDARLNF
                     NLSYNHLFQNDYTPLPGARVDRADGEIGTAADRFTGTLGYSSDRFKWSFTGTYIGKSY
                     EDDVNWCAFFGDGSPRCVSVPAEFYLDTQMSFLPTEKFEVYFGVDNLLDNDAPNILTN
                     TTFNITGSDTAADVYDIFGRRYYAGVRFHF"
BASE COUNT         4249 a         7287 c         7372 g         3982 t
ORIGIN      
        1 atcgaggtga tcgccgaggt gcccgacggc ccgccgcagc gtttccgctg gcgccgcgcc
       61 tttcacgcga tccgccgcta cgaaggcccc gagcgcatcg ctgccgaatg gtggcgccgc
      121 cgcgacaatt gcgggctgac gcgcgactat taccgcgtcg aggacgcgca gggccgccgc
      181 ttctggctct tccgccacgg cctctacgac gaaaagcccg acccgcgctg gtatattcat
      241 ggggttttcg cgtgagcgag gagaccgcga atccgggttt tgccgaattg gtggcggcga
      301 ccaattacag cttcctgcgc ggcgcctcgc atcccgccga gatggttgcc gaagcgatcg
      361 cgctcggtat gagcggcatc ggcatcgccg accgcaacag tgtggcgggg gtggtgcggg
      421 cgtgggcctt tctgaaagat ttgcaggtca agaagcccga ggtggtggaa ggctttcggc
      481 tggtggtcgg cgcccggctc gtcttcgccg acggcacccc cgatatcgtc gcttaccccg
      541 tcaatcgcga aggctggggg cgactaacgc gccttctctc gaccggcaat ctccgcgcac
      601 aaaagggcga ctgcatcctc gaactgggcg atttgctcga tcattgcgac gatctcttcc
      661 tcatcgcgat ggaaggcgac gaggagttgc tccgcaccct caaacgcaaa cgccccaagt
      721 cggtctggct ggcggccacg atgtcgcaat cgggcgccga cgcgcgccgg ctggccgaac
      781 tccagcgcct gtcggcggcg accggagttc cgctactcgc gaccaacgac gcactctatg
      841 cgaccaaggc acaacgcccg cttcacgata tcatcacctg catccgcgag ggcacgacgg
      901 tctcgaaagc cggcaagctg cttcgtgcca atggcgagcg ctatctgaaa gcccccgccg
      961 agatgaaacg cctgttcgac ggctattcga aggcgattga agcaagtgcc aagctgctcg
     1021 cacgtatcgg ttttggcctc gaagacctgc aatatgaata tccgcacgaa ccggttccac
     1081 cgggatggaa gccctttccc tatctgcacc atctggtgaa aaccgcagcc gaggaacgtt
     1141 acaaaacgcc gctcccgccg aaggtccgca agctgctcgc cgacgaactc cggttgatca
     1201 agaaacggcg ctacgtctat tatttcctga ccgtctatga cctcgtccgc cacgcccgtg
     1261 cgcagaaacc cccgatcctg tgtcagggcc gtggttctgc tgccaattca atcgtctgct
     1321 tcctgctcgg ggtaacctcg gtcgacccca tgaagcacga tctgctcttc tcgcgtttcg
     1381 tgtcggccga gcgtgacgag ccccccgata tcgacgtcga cttcgaacat gaacggcgcg
     1441 aggaggtcat ccaatatatc tacgaccgct acacgcgcga ccgcgcggca atcgccgcga
     1501 ccgtcatcca ttatcgcccg cgcagcacga tccgcgaagt cggcaaggcg ctcggtctca
     1561 gcgaggatgt caccgcccgg ctcgccgaca cgagctgggg gagttggggc gacgaggtac
     1621 cggtcgagcg actcgtcgaa gccgggctca atccgaacaa tggtgagatc gaacggctgc
     1681 accggttcgt cggcgaattg ctcaaagcgc cgcgtcacct gtcgcagcat gtcggtggct
     1741 tcgtgctcac cgaaggacgg ctcgacgaac tggtgccgat tcacaatgcc gcgatggagg
     1801 accgcacctt catcgagtgg gacaaggacg acatcgacgc gctggggctg atgaaggtcg
     1861 atgtgctcgc gctcggcatg ctgacctgca tccgcaaatg ttacgatctg atgcgcgagc
     1921 atggattggg aaatcacacg ctcgagctcg acatcgattg cgaggacaaa gccgtttata
     1981 aaatgctcca gcgcggcgac agcatcggcg tctttcaggt cgaaagccgg gcgcagatca
     2041 acatgctgcc ccgcctcaaa cctaaggtgt tttatgactt agtcgtgcag gtggcgatcg
     2101 tccgccctgg cccgatcgaa ggagatatgg tccatcctta tctgaagcga cggaacggag
     2161 aggaagaggt gaaattccct tccccggcag caccgcatga ccccaacgag cttcatgatg
     2221 tcttggataa aacctacggc gtcccgctgt ttcaggaaca ggcgatgaag ctcgctatcg
     2281 tcgccgccgg ctttaccgct gaagaagcga atggtctgcg gcgcgcaatg gcgaccttcc
     2341 gcaacgtggg caccatcgac aatttccgcg agaagatggt cggcggcatg gttgcgcgcg
     2401 gatatgaacc cgacttcgcc gagcgctgtt tcaagcagat cgagggcttc ggcagctatg
     2461 gctttcccga aagccatgcc cagtcgttcg ctcggctcgt ctatgtctcc tcatggatca
     2521 agcattacca ccccgccgtc ttcgcgtgcg gcattctcaa ttcgcagccg atgggcttct
     2581 acgctcctgc tcaactcgtg cgcgatgcgc aggggcatgg ggtcgaggtg cgtgaggtcg
     2641 acgtcaatgc gagcggctgg gacaatagcc tcgaacccaa ggccgacggc agcctcgcgc
     2701 tgcggctcgg cttccggcag gtcgacggat ttcgcgagga atgggcgaca cagatcgccg
     2761 atgcgcgcgc cacccccttt gcctccgtgg aagatctcgc gcgccgcgcg aacctgccgt
     2821 cgcgcgcgtt gcgtctgctc gccgaggccg atgcgtgccg ttcgatggga ctcgatcggc
     2881 gtcccgcgct gtgggatgcg cggcgggtgc ggcaaggggt gttgccgctg ttcggggcgg
     2941 ccgaaaccga tgagctcggc gtcgaggagg atgcacggct accgccgacc ccgctcgtcg
     3001 agcatgtgct gaccgattat cagacgacgc ggttgtcgct caaaggtcat ccgatggcct
     3061 tcctgcgccg cgatttcgaa cgcgagggcg tgctgagcgc ggccggggtc gccgcggcga
     3121 aaaatggatc gatcatccgc accgcgggcg tcgtgctgat tcgccagcgc cccggcaggg
     3181 gcaacgcgat cttcatcacg ctcgaggatg agggcgggat cgtcaacatc ctcctgtggg
     3241 cgcgccattt cgaacgccag cgccgcgcgg tcatggcatc gcggctgatg ctcgccgagg
     3301 gcgaggtgca gcggagcaag gaaggcgtca tccacctgat ggcgacgcgg atcgtcgacc
     3361 ggacggcgat gctcgatacg ctgggcagcg accgccgctt cgatcccgac gtctgccgcg
     3421 ccgacgaggt caggcatccg cagctgccgc gcgggcatgc ggcgcagcac ggccatccgc
     3481 gcaacgttcg catcctgccc aagtcgcgcg attttcattg aggcgggaat cgggcaggca
     3541 gttgtcccgc ccgcgcgacg gcaatggggg tggagagctg ccatcccctt cgtcattccc
     3601 gcgaatgcgg gaacccagcg agccggcgct gcttctgggt tcccgctttc gcgggaatga
     3661 cgaaggtggg aatggcggct ccccaccccg aagcgggcag ttcgagcaag cccgagaagc
     3721 cctcaatacg ggcttttcgg cgcggttccc gggcgggtga agagcttgcc gcgttcggcc
     3781 cagaaaacca gggcaagccc gaccaccgcg ctgatcagga aggcataagc cagcggcagc
     3841 gtcgatccgt catattgctg accgatcgtc gccccgaccg tcgccgcgag caaggtcttg
     3901 gcaaagctct gatacgacga ggcgacgccc gccatatgac cgaaatcctc catcgcgatc
     3961 gaaccgaaat tgctgccgat gaagccgacg agcccgacgt tgaccatcat cagcgcggtg
     4021 aacatccaca aggtctcggc gccgctcaag gccgcgacga tctgcaaaat cgaggtcgcc
     4081 atgaaggcga agaccgccga ctgcgacacg cgccgcgccc cgaagcgttc gacgatcgcg
     4141 gcgttcgaga aattggcgat cgcgatgccg accgcgacga tggcgaagat caacgggaac
     4201 atcgcccgcg ccccgaacac ctcggcgatg atctgttcgc tgctgttgag atagccgtag
     4261 agcgcgccct gcatcatccc cgacgcgatc atatagcccg ccgcgcggcg gtgccgcgtc
     4321 accgtcgccc agcccgcgac catcgtcttg aagtcgagcg gacgcacgtc ggcggggtcg
     4381 agcgtttcgg gcaggcggcg caaccagaac agcatcacga tcgacatcac cgcgagcacg
     4441 atgaagatcg cgcgccagcc ggccaccgcg gcaatgcccg cgccgatcgt cggggcgatg
     4501 atcggcacga tcatgaagac gaggaagatc agcgacagcc gcttcgccat cgcgtcgccc
     4561 gaaaacaggt cgcggatcac cgcgacgacg atcacgccga gcgcggcgct gaccagcccg
     4621 tggacgaaac gcagcgcgag cagcatcgcg aaactcgtcg caagtccgca accgatcgag
     4681 agcgccagat aggcgaagag cgccgggacg agcacgccct tgcgcccgaa ccggtccgaa
     4741 agcggcccgt aaagcagcga cccgaaaccg attccgagca gatagatcga gatgacatgc
     4801 tggcggtcgt tcgcgacggt gacgcccagc gcttcgccga tcgcgggcag cgcgggcagc
     4861 atcgaatcga tggcgagcgc gttgagcgcc atgaccatcg ccatcatgac caccatttca
     4921 cggtcgcccg gcaggctccg ttggggggat tgggccggct tgtgcatggc agcagcctat
     4981 gcgcccaagc cgccgctttg gatagccccc attggtccgc aaagccggaa gggcattgct
     5041 atgcaaagca aaagggccgc ccgatcaagg cggcccttcg cttgtttcgc cccgacgggg
     5101 tcagccctgg cgcgtgccgg tcatcgccat cgaaccgaac gcgccggcct tgatcgtgcc
     5161 ggtcagcgcg tcgccgtcga tcgtcgcttc gcattcgagc gtcatcggca tcggcacctt
     5221 catgttcatc gtccagctca gcttgttgcc gtcgaccttg ccattttcga catcgagcga
     5281 ccccatcgcg ccggcattct ggccggtgaa gctgtcgccg ttgctggcga cggtgaaaac
     5341 cgacttctgg tcgcccatcg gcgacttggt gacgcaatca tagctgccat cgacttggga
     5401 catctgcgct ctccttactg aaactgatgt tagttagcgg gagacaccgt ttccacgggc
     5461 agacccaatc cgtcaagctg cggtttcacc acggcggcgt cgccgacgac gacatagacg
     5521 agatcgtcgg tgctgagcgc ctttcgcgcc gccgcatcca acgcttcggg cgtcatcgcc
     5581 tcataggttg cgggcagctt ttcgtaataa tcgtcgggac gatcgaattt gacgatcgcg
     5641 cgcagcccgc cgagcacgtc gctcgaggtt tcgaaactgc cgggcagttc gcggacgctg
     5701 ccgttcacgg tgcgctgcaa ttcctccttg gtcacgccct tgtcgccgag gaaggccttg
     5761 aggtcgcttt gcagctcctt gatcgaatcg cccgtgcggt cggcctgcac cggcgccgac
     5821 gcgatccagc tgacgcggtt cgtctcgccc gacacgctcg tgcgcacgcc ataggaccag
     5881 cctttcgtct cgcgcaggtt catgttgaag cgcgacagga aattgccgcc gaagatgtcg
     5941 ttcgccgcgc gcagcacctc cagcccatcg ccgcccttgg cgtcgagcac cttgcccgcc
     6001 aggatcaccg attgcggcga tttgggccgg tcgacgagca ggatgcgcgg ctgcggcttg
     6061 gggaccgcga cctcgaaatg cttcgtcggc ttcggcgtcg ccggcgcttt ccagtcgccg
     6121 aacgcggtgt cgagttcctt cttcacctcg gcgagcgtcg tgtcgccgac gacgaagatc
     6181 cgcgcattgt cggggcgaat ccacgcagaa tggaaggccg cgagctggtc gcgcgtcgcc
     6241 gcggtcaccg cttcggcatt gccgaggccc gagggcggaa tgccataggg gtgctcggcg
     6301 ccgtacagcg cgggcatcag cacgcggctg gcgatcgcgc gcggttcgtt gagttcggcc
     6361 ttcagccggt tgagttgctg cgcgcgcacg cgctcgagtt ccttcgcgtc gaacgccggg
     6421 ttgcgcacat aatcggcgag cagcgcgagc gacgcgcgca aattcggctt gagcgcgaac
     6481 agcccgagcg cagtttcgtc ggcgtcggca tagccatagg tctgcgcgcc gagccgctcc
     6541 ttcgcttcgg cgaaggcgat cgaatcgagg ctggtcgtgc cctcgtccat caggctgagc
     6601 atcagcgatt gggtgccgag cgcgctgcgc ggatcggcgg catagccggc gtcgaagctc
     6661 acctgcacat tgaccgtcgg caccgtcgtg cggcgcgcga aaaccacctc gatcccgttc
     6721 ttgagcttgg cgcgttcgat ctcggggaat tcgagcgcct tcaggcctgt cacactggga
     6781 aattgcgaac ggtcggcgat cgcagtcgcc gcgccgatcc cgctgtcggg accgacatcg
     6841 ccgagcgcgg ggttccagta gcggtcgggc gcgactgccg gagcaccctt cgccgcgacg
     6901 acggcgccgc cgcggtgctc gccgccttcg gtgcgttcgc cgggcgtata ggtcagcgag
     6961 aaggccgggc gcgacagcca tttgttcgcg gccgccttca cctgctcggg cgtcgccttg
     7021 gcgaggcgct cgagctcgac tttataatag gcgggattgt tcgaatagag cgcgccctcg
     7081 gcgagcgtca ctgccttgcc gccgaagccg ccgaccgatt cgagctgcga gatcgcgccg
     7141 ccgagatagc tggcggtcgc gcgctgcaac tcgtcggcgg tcggcccgct cgcgaggaag
     7201 gccgcgattt cctcgtcgag ccttttcgcg acatgcgcgg gatcgacacc cgccttgacg
     7261 tcggcctgga ccagcacgat ccctgcatcc tcgaacggcg atgcacccgc cgagacgctg
     7321 accgcgatcg ggtccttgcg cacgagcgcg ttgtcgagcc gcgacgagga cagcccgccg
     7381 agcaccgaca tcgccatctg gagcggcacc gattcggcat cgttgagccc cggcatcgtc
     7441 cacatgcgat agatgcgcgt cgtcgggatc aggtccttga cctccttcgc cagcggcgcg
     7501 ggcagcgtcg gcagcgaggt cgcgggcgcc ttgatctcgg gaccgcgcgg aatgtcgccg
     7561 aaccattcgc cgaccttggc tttggcggtc ggcacgtcga tgtcgccggc gaggacgagc
     7621 accgcgttgt tcgggccata attgtcggcg aaccacttct tcacgtcatc gaggctcgcc
     7681 gcgtcgaggt cggccatcga gccgatcgtg ctgtgatgat aggggtggcc cttgggaaag
     7741 agattctcga aaatctcgta gcggagcagg ccataggggt tgttgtcgcc ctgcctcttc
     7801 tcgttctgga cgacgccccg ctggttatcg agcttctcct gcgtcaccgc gccgagcaga
     7861 tgccccatgc ggtcgctttc gaggaacagc gcgaggtcga gcgcgccggt cggcaccgtt
     7921 tcgaaataat tggtgcgatc gaggaaggtc gtgccgttga aatcggtcgc gccgacctgg
     7981 cgcagcggct cgaagaaatc attgggggcg ttttcggagc cgttgaacat cagatgctcg
     8041 aacagatggg caaagcccgt cttgcccgcg ggttcgtgct tcgacccgac gcggtaccac
     8101 accgacaccg cgacgaccgg cgccttgcga tcctcgtgca cgatgacgcg caggccattg
     8161 tcgagcgtga aggcttcgta cggaatatcg accgccttga cgaggtcggc ggtgggcgcg
     8221 ggcgccgcgg ttttggcagc actccgggcg aacgccggag cggtggcgac gagcgaggtc
     8281 gacagggcaa gagcgacagc gaaacggtgg aaacgagcca tgaaaatccc cagatcaagg
     8341 cgcgcaggcg gacgcctgct caaaatcggg tgccggacgg cactgtatca atggtttagg
     8401 gccgtgctcc gcggctttca aggccgatag aagcgcgcgc cgctcgcctt cagtgccgcc
     8461 ggatcgaaca gcacgggctt gagcttgtgg gccacgaaca gcggcgcctg atcattgtaa
     8521 tgcgggctgt cggggcgcgt cgtcgccgcg ccgaaaggct ggatcgattc ggagcggacg
     8581 cggccgttcc ggtcccacgt cacgaacatg atgaagctgt cgccgtggcg caccttcagc
     8641 cgcccgtcag gctcctcgtc ccacagggtc gaagcgcgca ccgtgtcgtt gccgccgtcg
     8701 agcggcaggt cgacgcggtt cgcgccctcg ccatggcgga ggcgcagcac ggtgccgagc
     8761 ttcggatcga gcccgttgaa atgctcctgc agatgcgcga ccgtctcctt gagcacggtg
     8821 cgcggatcgg gcgcggcgcg gcgctggtag tggcggccgt tcgccgggcg cagcagcatc
     8881 agcgcgagcg cgtcgccttt gccccggccg tcgagagtcc agtcccattt gcgcagcagc
     8941 cgctgggctt cggcgagcgc cggatcgccc ttcgtatcga gcgcgagcag ccggtccatc
     9001 cacgctttcg catagccgct ccgcgaatag gcggtgtcat atttgatcgc cttgagccgc
     9061 gcctcgtcga tctgtcccga cgcctcgaac agctcgatca gccgggtcgc gcgattggtc
     9121 atatcgtcct cgatgccaag cagcggcgaa aaggccgcgg cgtcgagttc atcgcccggc
     9181 cccgccgcga cccacggcgt gttgttcgcg ttcatcacat agcccgaacg cggattgacc
     9241 agcgcgggca cgcggtcgaa cggcaacgtc ttcgtccaca ggtcggccga tgtgtcaccg
     9301 ggcagcacgc cgcgccagtt gaaccccgcc ggccggtcgg ggaacatcgc attgtagaag
     9361 agcccgatat tgcccttcgc atcggcatag atgaaattgg tcgcgggcac accctgcccc
     9421 gccatcgccg cgcgccattc ggcgaaagtc ttcgccttgt tcagccggta atattgggtg
     9481 accatattcg cctgatcgat cccggcgtaa cggatcgcaa aggcgccctt ttcattcttc
     9541 acgaccggcc cgtggaccga gcgccagatg gtgcgcggca ccggcagcac gaaggggccg
     9601 aagcggacct tcagccagat gcgcttttcc tcgagcgggc gccacgcgcc gtcgaagcgg
     9661 taattcttgc cgctgtcgtc gaggaccagc ttgtaaacgt cgatcaggtc aggccgattc
     9721 accgtgttcg tccagccgag atatttattg tggccgagga aagggtaagg cgagccgggg
     9781 aagttggcgc cggcgaaatc ccagtcctct tcgctatgga cgaccagctc gtaccacgcg
     9841 accccgccgg tccacggctg gtgcgagttg gagacgagcc gcgtcgcgcc gtcggtcgat
     9901 cgcgccggcg cgaccgccat cgcgttcgac ccgttatcgg ccgggtcggt gcccaccggc
     9961 gtcagttcgc gcgggacgag cttgcccgac ttgtcgagcg cgggaccgcc ctcgcgcccg
    10021 atctcggtgc cttcggtgac cgaaccgagc accgaatcga gcccgaagaa aaagggcgag
    10081 cgcagcacga agcccgcgac gacatcctcg cccgtgacgg ggaacagccc cgacagccgc
    10141 acctcatccg gatgcttgtc ggcataatgg ttgagaccag cggcataagc cgtgaacagc
    10201 tttttcacat cgtccggaag ccgcggccag tcgcgccgcg tcgtcgcgcg gatatcgagc
    10261 agcgccgcgg catagtcgat cttcgcccca tccggcccaa gcatcgcgcc ggcgcgcccg
    10321 cgcgtcatcg cgagcacctc ctgcagcgtc gagaaatcat cctcggcatg cgcataggcg
    10381 acgccatagg cgacgtcggc gtcggtcttt ccgaagatat gcggcacgcc gaaattgtcg
    10441 cgcgcgatgc ggacgtccgc gggcttgaag gcgggagccg gcggcgcttc ggcggtcagc
    10501 ggctcccaga aagcaagcga tacggcggca acgacgatca gagccagcaa gcccagtaaa
    10561 atccgtcgta gcatcgtggg tcctcttcgt cgccggtccg ggcgcatctg tgaccgccct
    10621 gcccgccgat ggcaagcggc tgtcgttcgt cgaggcggat ttgcggggcg gatagcgcgc
    10681 cgcgacaata gagttctgcg gaacctcgga aggccccttg tgttgcacaa tagcaacact
    10741 atcttgctgg gcaagaaagg ggcttgtcca gtatgaacct cgaaaaattt accgaccgcg
    10801 ccaagggctt cctccaagcg gcgcagacga tcgcgatccg catgaaccat cagcggatcg
    10861 cgcccgagca tatcgccaaa gcgctgctcg aagacaatca gggcatggcc gcgggactga
    10921 tcgcgaacag cggcggcgac accgcgcgcg cggtggccgg catcgacgcg ctgctcgcca
    10981 agctccccgc cgtgtcggga tcgggcgcgc aggcgacccc gggcctcgac aatgacgcgg
    11041 tgcgcctgct cgatcaggcc gaacaggtcg ccgcgaaagc gggcagcgaa ttcgtcgccg
    11101 tccagaatat cctgctcgcg atggtgctcg ccccgagcac gccggtgggc aaggcctttg
    11161 ccgacgcggg cgtaaagccc gaagcgctca atgccgcgat cgccaagctg accggcgggc
    11221 gcaccgccga cagtgcctcg gccgaggatc gttacgacgc gcttcagaaa ttcgcccgcg
    11281 acctcaccga agtcgcgcgc gagggcaagc tcgaccccgt tatcggccgc gacgaggaaa
    11341 tccgccgcac cgtgcagatc ctcgcccgcc gcaccaagaa caaccccgtg ctgataggcg
    11401 agcccggcgt cggcaagacc gcgatcgccg aagggctcgc gctgcgcatc gtcaacggcg
    11461 acgtgcccga cagtctgaag gaccgccgcc tgctcgcgct cgacatgggc gcgctgatcg
    11521 cgggcgccaa atatcgcggc gagttcgagg agcggctcaa gggcgtgctc gacgatgtga
    11581 aggccgccga gggcgagatc atcctgttca tcgacgagat gcacacgctc gtcggcgcgg
    11641 gcaagggcga gggcgcgatg gacgcgtcga acctcttgaa acccgcgctc gcgcgcggcg
    11701 agctgcactg catcggcgcg acgacgctcg acgaatatcg caagcatgtc gagaaggacc
    11761 cggcgctcca gcggcgcttc cagccggtct tcgtcggcga gccgacggtc gaggattcga
    11821 tctcgatcct gcgcgggatc aaggaaaaat atgagctgca ccacggggtg cggatcaccg
    11881 acggcgcgat cgtcgccgcg gcgacgctgt cgaaccgcta catctccgac cgcttcctgc
    11941 ccgacaaggc gatcgacctg atggacgagg cggcgagccg catccgcatg gaggtcgaat
    12001 cgaagcccga ggcgatcgag agcctcgacc gccgcatcat ccagatgaag atcgaggaat
    12061 cggcgctggg caaggaaagc gacgccgcgt cgaaggatcg gctcgcgagc ttgcaggccg
    12121 agctcgccaa tctcgagcag cagtcggccg aactcaccca gaaatggcag gccgaaaagg
    12181 acaagatcca cgccgaggcg aagatcaagg aagagcttga cgccgcgcgc ggccagctcg
    12241 accaggcgca gcgcgccggc gatctcgcca aggcgggtga gctcagctac ggcaccatcc
    12301 ccggcctcga aaagcggctc gcggaagcag aggcggccgc gggcaatgcg atgctgcgcg
    12361 aagaggtcac cgccgacgac atcgccgccg tcgtgagcaa atggaccggc atccccgtcg
    12421 accggatgat ggaaggcgag cgcgagaagc tgctcggcat ggaagacacg ctgggccagc
    12481 gcgtgatcgg tcaggacgaa gcagtgcgcg ccgtctcgac cgcggtccgc cgcgcgcgcg
    12541 ccgggctaca ggaccccaac cggcccttgg gctcgttcct cttcctcggc cccacggggg
    12601 tcggcaagac cgagctcacc aaggcgctcg cgcgtttcct gttcgacgac gacaatgcga
    12661 tggtgcgcat cgacatgtcc gaattcatgg aaaagcacag cgtcgcgcgc ctcgtcggtg
    12721 cccccccggg ctatgtcggc tatgaggagg gcggcacgct caccgaagcg gtgcggcgcc
    12781 gcccctatca ggtcgtgctg ttcgacgagg tcgagaaagc ccatcccgac gtcttcaaca
    12841 tcctcttgca ggtgctcgac gacggccgcc tcaccgacgg gcagggccgc acggtcgact
    12901 tcaccaacac gctgatcatc ctgacttcga acctcggcag ccaggcgatc gccgcgctcc
    12961 ccgacgacgc ccccgtcgaa caggccgagc ccgcggtgat ggaggtggtg cgcgcgcatt
    13021 tccgccccga gtttctgaac cggctcgacg agatcgtgct cttcaaccgc ctcgcgcagc
    13081 agcatatggg cggcatcgtc gacattcagg tcgcacgtgt ccagaagctg cttgccgatc
    13141 gcaaggtgac gctcgatctc accgacgccg cgcgcgcgtg gctcgggcgg gtcggctatg
    13201 atccggtcta tggggcgcgg ccgctcaagc gcgcggtgca gaaatatctg caggacccgc
    13261 tcgccgacct gatcctgaag ggcgaggtta aggatgggca gacgatcagg gtcgacgaag
    13321 gcgacggcgc gctggtgctg acccctgttg gctgagccgg gtcagcccgc ccgcatcagc
    13381 gtccgcgtca attgccggcg ggacgtgtgc ggcgatcctc gacaaagcga ttcagcgttt
    13441 cgttcagttc ggacgaggcc ttgctccatt cggacttggt ccggcagact ttctttttgc
    13501 gcaccagcga tccggttacg tcatatgttt tgcagatgac cggatcggcg ggtttcgcgg
    13561 gctccgccgc cggcggcgtg tcggcgcccg agccgagcaa caggaacgca agaaggaaat
    13621 gagccatcga tcgaccctct gttaccggct gtcagcccat gatagcgtca ggttcgcggt
    13681 cgatcaatca tcgacgctgc cgccagcatc gaggccataa tcgaccattt cctggaacag
    13741 gccgcgcaac atctcccgct cggattcgga aagatgcggg ttccagacat cgacgatcag
    13801 gatcgcgcgc agcgcatcgc tccggttcca tgcttcatgc tcgatcgtgt cgtcgaaaac
    13861 gaacgcctgc ccctcgaccc agtcgcgcgt ttcgccgccg acgcgaaacg cacagcgttc
    13921 gggcacgatc agcggcaaat ggacgatcgc gcggacattg gtgacgcccg tatgcggcgg
    13981 aatatgcgca ccgggccgga gcaacgaaat gaacgccgtc ggcgaacgcc ccggaatatc
    14041 cgagagcggc agctgatcga gcaccgccgc ggtccgcggc atgcgcgcgc aggcttcgtc
    14101 ctgacgcacg ccgtgcttcc acaggaagtg gacgctccag tcgggggagt tgtcgagcgt
    14161 ggtccatttg ttcggcgccg ttccgggttc ttgacgaaca tagggctcga gcccttcggt
    14221 gtcggttgcg agcatcgcca gcagttcggc gcgaatatgt tccgtctccg cttccagttg
    14281 ttcgatccac gggaaatggc ttcggtcgaa gaattcgtcg gcgggcagaa aggggtaatg
    14341 cagaccgtgg cactcattgg cgtaaatgcg gcggcgcccc aaaatggtat cgacgcaggt
    14401 ctcgaaccgc cgcatcgcca acggctcgac gccgttccgg cgaaaggccg caaggcgcgg
    14461 agacaggcta ttctcgaaac cgctcgcgac accggcgata tagcgccgcg catgttccag
    14521 caaatccgcg agcgaggcat tggccgtccc cgacgcctcg gccagcgcga tgacgcccga
    14581 ccagcgatgc atcgccgcgg cggtctctcc gcgcttctcg tgcagttcgg cgcggcgcac
    14641 attcgccatg aaatgacgct ggtcgatcgc cagcgcgcca tcgaggcttt cggcttccag
    14701 cctgtcggcc tccgcgacgc gatgcacctt cgccaggttc atccataatt cgggcgccgc
    14761 ggcatcgttt tcgatcgccc gctcgaaata atcgcgcgca tcggcgagtt gccgggcgtc
    14821 gagcgcgatc atgccaaggc tgttgagtac gaaagggtcg ccgggtgcgc gcgaaagggc
    14881 ggcctgcaaa tgatcgatgg cccgccgggt atcgccggcg ttccgtgctt gcgcagcggc
    14941 agtcaaaaga tcgcgtacat ccgaatggct catgtcgccg cgataaccga cccctgtcgg
    15001 tgaggcaaaa aaaaaggcgg gcacaaggcc cgcctttctt tttttggcgt cggatcagaa
    15061 cttcagacgc gcaccaaccg catagcgacg gccgagggcg tcataggtcg acggataggt
    15121 gttgccgctg ttgtaggtgg tcgaaccgat gttcgaacca accgtcggcg gatccttgtc
    15181 gaacaggttc atgaccgaga gcgtcagttc gaaattctcg gtcacgctcg cccgggtcgt
    15241 cagatcgaac cagttgaagg ccttgatctt ccggaagggc tcgaaagccg gaccgttggt
    15301 gccgaactgg gtttcgtcag catcgaaggg ttcctgcttc gtgctcgaga tgtgacgcca
    15361 cagaagcgaa acgtcgatgt cgccgaacga cagcgtcgta cgctggttga acgagaactt
    15421 cggcaggatc gaaccacccg aaaggtccga accggtgggc gtgcagttga tgctgtaata
    15481 gcccacgcat tcacggttca ggccgcccgg cttcgaacgg aacttcgaat ggaaggtgta
    15541 gttaccggtg aagccgagcg acagggcgat gtcgtcggtc agatcggtct tccagttcgc
    15601 agcaacgtcg ataccgtcgg ttgcaagacg cccgctgttg gtcagcgcca gcggaacgcc
    15661 gggcacggtc gcagccgaac cgtcgagacc accgatcgcc gggtcgcgct ggatcgacgt
    15721 acaggccacc gaagtcgcgc tgtccgcggt caggttggcg aagcagtcgg agagaacgtc
    15781 gccgaccgtc gggaacgaaa tcgcatcatt gatgatgatg ttatagtaat cgaccgacag
    15841 cgtcaggttc ggaatgaagc tgggctggaa gatcgcaccg accgtgaagg tatcggcctt
    15901 ttcaggcttc agaggcgccg aatagtcgaa cgatgcgttg atctgtccgg ccgacggcgg
    15961 gtcgaaggcc gcaatattgc cggcgccatt gaccaatgcc ccctgcgcga cacaaacctg
    16021 accgaggttc gacgtggcgt cgattgtggt accgtcgctc tggcagggat cacgcttcag
    16081 gttgacgaga ccaaccgcat tcggcgtgaa aatctcgccg atgttcggcg cgcgaaccgc
    16141 ccgctgatag ttgccgcgaa gcttgaagcc atcgaacgct tcccaggtcc caccgacctt
    16201 gtaggtccag gtgtcgtacg actggtcggt ggcgtcatcg acctcatatt tcgaatagcg
    16261 cgcgccggct tcgagcgtca ggcccgcgaa gagcggcgcg ataatttcgc cgaagacttc
    16321 gcgaacattg tagccgccct tgaccggagt ggtcgcgccg ccggcgccgc ccagttcgcc
    16381 gggcgattgc gcaagctgat cggcgaagac ttcagcggta tatttgcgat attcggcgcc
    16441 cacagcgaag gagatcgcat ccgagctctc ggaaaacgat ccgagttcgc ccgaaagcaa
    16501 gccgcgcacc tgcgccagcg aggtacgatt gcccgaggtt gccggcacga gcagatagtc
    16561 gagcatttcg ggcgtgatcg aaccgctggg accgaaaacg ttcacgggaa cgcaaccgcc
    16621 cgccggattc tggcagttgg ccgcgtcggg cgcgcgcagc gcctgctgca accgcgtcag
    16681 cgacgcatag ttcaggatgg tctgggtatt ttcgctttcg ccatagttac ccgaaacttc
    16741 ataccgaagc gtgtcggtga tcgacccgcg gacaccagcc atgaaatcga acagctgcgt
    16801 cttgtagctg ctgacgcgac cgccggcttc gacgaggcgg cgccccaggt tggtcgaaac
    16861 ttcctgataa ttggggtcgt tgggatcggt cgccgtcgcg gccgcggcgc actgcgcccc
    16921 cgtcagcgtc tggataccat ccgtgtccgg atcgaagtcg ttgttcgcgc agaaggtgtt
    16981 gcgcgcgccg gccgtcatga acgggttgct gagcggaatg aaaaccggat cgctgaacgc
    17041 gcccgacggt gcgatgatcg tctgcaccgt attctgcgag aacatgccgc gcgcgaacac
    17101 ttcaaccgaa tcgctaacct cgtaacgcgc cgaggagaac atgttgtacc gctcgaacgg
    17161 cgtctggaaa atgttatagg ggttgaagtt gaacagcgcg aactgcggca cgaggttgcc
    17221 cgtcgccgga tcgatctgca gattgccgac gccgggaatc gagaaacgcg acggcgatgc
    17281 ggtgcccgat gcgccgctgc ccgaaccatc gaacgaatcg atggtcaggt ccgaacccgg
    17341 ccgttcgcca ccgaaaaaca ccggatcggc tttctggtaa ccgacactga gaaccgcgtt
    17401 accgcggccg tcatcgaaat tcgcaccgac cgtcaggtcg gcacggaaaa tccggccatc
    17461 gccgcgttcg gtgatctgat agctcgtcgc aaggtcgacg ccggcgaaat cgcgcttggt
    17521 gatgaagttg acgacacccg aaacggcgtc cgcaccatag gtcgtgctcg cgccgccggt
    17581 caggacatcg acgcgttcga taagcgccag cgggatgttg ttaaggtcga cgttgccgtt
    17641 cagattggcc ggcgcgatcc gctgactgtc gagcagaacg atgttgcggc tcgggccgag
    17701 gccgcgaaga tcgacgaacg aagcgccgcc gttgccgttg ttgaccgacg aaccgacgtt
    17761 cgcggcgacg ccgggaacgt cgcgcagaat atcttcggca acgttaacct ggcgcagctc
    17821 gatttcgtcc gcagacgtca cgttgaccgg gctggaggtg gtgaggttgg ggttgcggac
    17881 cagcgtgccg gtcacgacga tctcagaagt gacgtcggtc gcgccttcgt cggcggcgtc
    17941 ctgcgcatag gcgggagccg aaagcagggc agcgctcatc acgaacggcg cggcgcccag
    18001 cttcagcttg gaatagtggg ttttcttcac agtgcagtcc cttgctactg gtggatgaat
    18061 tacccggacc ccccgatacg ctgccccaat aggcagcgca aagacgtccc gatagtgtgc
    18121 gggatgactg ctcgttcatc gggagtaaag ggagattccc ctgctttccg tcgtttcaaa
    18181 ccccattgtg acgtatttgc aacaaatgcc gaacggcacg aaatacaatg gcatgcaggc
    18241 tggtatcggc gacattttat attacggccg caccttcttc agcgaggtgc agcggcccgg
    18301 cttgaccatg cgccgggcat tgataatgtc gcatatgggc tgaacgcacc gcgaccttcg
    18361 ttgcgccgac gatgacgcca gaaggggttt tgtcgatgaa gaattcggtc ttgtgggcga
    18421 gcggcctcgt cgcggcggca ctcggcgcga ttgcggtacc cgatgcacat gcacaggata
    18481 tgtccgggga cattctgcgc tgcggcgcga ttgcggataa tccggcgcgc ctggcctgct
    18541 tcgacggact gctccccaaa gcgcgtcaga accacgctga tgccggggcg aaaagcgaag
    18601 ccgatttcgg gctgaccgaa gtgcaacgca acgagaagat caagcgcgat gcccccgaac
    18661 ccttggccgg ccgggaacgg gcgccggcga ccgagccgcg cgtcgaggcg acgatcgcgg
    18721 caatcgacat ggaccggttc agggccgttt tcacgctcga taacgggcag gtctggcaga
    18781 ccacctcctt tggcacgctg aacaccgttc cgcgcgccgg tcaaaaggtt gtggtccaaa
    18841 cggggccgct cggcggctat cgcctgacac tcgagggaaa gaagaacgaa atcggcgtca
    18901 agcgcgtaaa ataggccggc ccggcgtcac gcgcgccaca tcccgcccgc cacatcccga
    18961 tagtcgcccg gatgtccccg gctttccgga ccgcatcgac gcgcgacgcg acaatcgacc
    19021 caaagcggcc cgcccgcccc gcgcgcgcgg atcgtgccgg atggctcagc cgccgtcccg
    19081 atcgattgcc cgaaggcgtg aacgacgcgt ccggcagttg aagcgccccg cattcgcgga
    19141 gaccgggaca ccccaatggc ttcgaccttg agctcgcgca gccttgcggg tggcgatacc
    19201 atccgcggcg gtcgcatccc gcccgctatc ttcaccatcg tgccccgaac gcgcgtgatt
    19261 aacgggcttc agaccctagc ccgaaaaaga ccccgggcga cgcgtcagca cccgttcgaa
    19321 cagtggctcg agcggttgcg atcgtccatg gcctgacgcg cggcttcgcg ttcgcgcgcc
    19381 cattcctcgt tcgttttgca cacccgcttc ggaatccgcg agcccacctc cttgatcgag
    19441 cggcagcgga ccgtgctcgg gtccgcttcg gtcttgtctt cgctcgcaac cgcgaaaccc
    19501 ggtagcgaca ggctcagaac cgaagcgagc aagagaaggg agcgatacat ggctgaaacc
    19561 tcaaaaaaaa atggggaggc agcgacgaag tgctgcctcc ccgatattat ccggtttttc
    19621 cgaaatcaga agtgaaaacg gacacccgcg taataacggc gaccgaagat gtcatagaca
    19681 tcggcggcgg tatccgaacc ggtgatgttg aacgtcgtgt tcgtcaggat gttcggagca
    19741 tcattgtcga gcaggttgtc gacgccgaaa tagacttcga acttttcggt gggcaggaag
    19801 ctcatctggg tatccagata gaattccgcc ggaaccgaga cgcagcgcgg cgaaccgtca
    19861 ccgaagaagg cgcaccagtt gacgtcatct tcataggact tgccgatata ggtgccggtg
    19921 aacgaccatt tgaacctgtc gcttgaataa cccagcgtac cggtaaagcg gtcggccgcg
    19981 gtaccgatct cgccatcggc ccggtcgacg cgcgcgccgg gaagcggcgt gtagtcgttc
    20041 tggaacagat ggttatagga gaggttgaag ttcagacggg cgtcggtgtc gaagaaattg
    20101 accggcgtga accagttgac cgtaacgtcg agaccctggg tttccagtcg cgcggcgttg
    20161 accgcgagat cgttgatgaa ctcgatcgaa ccggcgctgt tggtcgccgt atcggtcgga
    20221 cggcggatga tcaggtcgca cagcggatca ttgccctgat tatagcactg atcgaggatc
    20281 gtgccgcgac cgacggtggc gatgatgttt tcgatcttga tgttataata gtcgaccgtc
    20341 agcgtcaggt tgcgcagcgc atcgatcgag cgcggattga gcaccgcacc gaccgtccac
    20401 gaatccgcct tttcttcgtt cagattcggg ttgccgccgt tgaagccgct gatgccctga
    20461 atgtcgggct gggtcagcgt gaacgtgccg ttcgcggcga tgttcgccgc cacgcccgca
    20521 tcggcgcggc aattgtcgcc gagcgcacca ccaccggtgg cgccgatccc tacgcaggga
    20581 tcctgaagac ccgacgggaa agtctgcgac aggcccgaga acagctcgcc gatgttcggc
    20641 gcgcgcacgg cgcgtgccca agtggcgcgc aggcgcaggt cgtcgatcgg ttcccaagtg
    20701 ccctgcacat tgtagctgta gatcgttccg accgtcgaat aatccgcgac gcggaacgca
    20761 ccaccgactt cgagcagctg gaagaagggc gtgtcgcgca ggatcggcac cttgatttcg
    20821 gcataggctt cggcgacgtc gaaccggccg ctcgtgtcgg gcagcttgtt gccggcgttc
    20881 agaccctggt tggtcagcgc gtcattgtcc tcgacgcttt cttccttgcg atattcaaag
    20941 ccgagcgcga cgccgagcgg accggcgggg agctcgaaga gcgaaccgct gatattgccc
    21001 tgcacgacct gctgcttgat gtcggtctgg agcgactgct gtgcagcgat atactcgatc
    21061 gcctcgggcg tgatcgcgcc cttgccgaaa atgttgatcg gcacacagcc attggcgcgc
    21121 gcttcggcgt tgacgcaaac cacgtcggtg gtcaggccgt tgccgttgac gtcgtcgacg
    21181 tcggtcatcg ccgcaagcgc gttgcggaaa ttgacgacgt tgacctggcc gttcgacacc
    21241 tggttctcgg tgacctgacc atagttgtag ctgaggtccc agttgaactt ttcgcccagc
    21301 gtgccctcga acccgaccac gaagcgcagg aaatcgcgcg tcgtctggcc gttgcgggta
    21361 ccgaattcgg tcaagcggcg tgcgaaaccg atatcgcgca gcccgtcgcc gtcgctatcg
    21421 accgcctgcg cggcgatcgc gccgggaacg atcggattga tcgcgatcga ggtcgtgccg
    21481 tcgggcgcga tgatgtaatt ctcgatcggc atcacgcccg aagagggata gacgccagtc
    21541 gaaccgtccg aagcgagcgc gaacggctcg atgatccgcg acgactgcgt cttgttgtag
    21601 gtgccctcga agaacatcga gatattgtcg gtgacgtcat aatgaccgac cgcggcgacg
    21661 gtgtaacgct tcaccggcac ggcgagcgtg cgataatatt ggcggttgaa gccgttcggg
    21721 ccaacgcccg tacccaggtc gctgctgcag gtcggaccgt tggcggtgaa gcagggctgc
    21781 agcacgccgg tcgggctata ggtgaaggta tagtcacccg acgtgaagct cccctgctcc
    21841 gagaaacccg aatagaaggg tccgctcgac acgccataat cggcgagatc gccggtcagc
    21901 gccgcagtgt cgctatcgtc ggtgaaggtg ttcttgcgct ggcgcgaaag cagccccttc
    21961 tcgttcgaat agccgaggtg aaccatgatg ttaccgcggc cgtcgtcgaa attcgcaccc
    22021 atggtcaggt tcgcctggta gcgaatatcg tcgccacgct cggtcacgcc atattgggcg
    22081 ttgccttcaa ggccctggaa atccttcttg tagacgaagt tgacgacgcc cgcgaccgcg
    22141 tccgagccgt agagcgacga agcgccgccg gtgagcacgt cgacgcgctc gatgaactgg
    22201 gtcggaatga cgttaaggtc gaccgtcgag ctgccgctga ggctcgacac gacgcggcgg
    22261 ccgttgacca gcaccagcgt gcggttcgag ccgagatcgc gaaggtcgat cgtggcgacg
    22321 ccggtgcccg acgtcaggaa ggccgagttg gtgcggctga gcgtcggcgt accgaacgcc
    22381 ggattcttga gcagcagttc ctggacgttg gtcacgcccg actgcgtgat ggcttcgctg
    22441 ccgatgacct gcagcggcgc attcgattcg gcctgcgggc tcgcgatgcg cgaaccagtg
    22501 acgatgatcg tgtcttccgc ggatgcatct tccgcgggcg cttcctgcgc gaagctggcc
    22561 gtcgaaagca gcgcaacgca aagcgccgcc ggcgccgccg ctgctctcaa tgcggtaaag
    22621 gaggtttttt tcacagtgca gccccttgct actgatggtt gagaatcgct cagggacatg
    22681 cacccgcaaa aattgcgctg catagaagct ccccggtaag gccgggatga cccgtcattc
    22741 atcttgagta aagggatttc ccggcgcacg tgcccgtttc ggacgtgatt gtgacgtatt
    22801 tgcaacagcc gagagaaatg gcgaaacaca aagggctagc taagcccctt tggtgaaatt
    22861 ttgtttcatc ggttacgagt tcgcctcggc
//
LOCUS       DATMSU010000306         1703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_157075,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000306 DATMSU010000000
VERSION     DATMSU010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157075"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VN034_08485"
     CDS             <1..576
                     /locus_tag="VN034_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HWV60675.1"
                     /translation="LILGAEPGATIALGTRAPVDRERLRAAALDGSIEELLDWKPVKA
                     GDFFYVPSGTVHAIGAGLTLIEVQQNSETTYRLYDYGRPRELHLDDGIAVADPRPYTA
                     YPSPGRVADDREILVDGPKFVLERLAGATRTVALPDGVTAWLIPVAGNGLADGVNFAA
                     GECLALTGTVQIAADAGADLLLAYPGEGRLP"
     gene            573..>1703
                     /gene="gluP"
                     /locus_tag="VN034_08490"
     CDS             573..>1703
                     /gene="gluP"
                     /locus_tag="VN034_08490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HWV60676.1"
                     /translation="MTAGRSGTGWTFAYVTMLFFVWGAVTAVNDILIPAVKAIFALSD
                     TESFLTQFAFFMAYGVVSLPAAALMGRLGAANSIIVALATMIVGCLIMPLATVVREYA
                     MVLVGLFVIASGITLLQVAANPLSASLGRPERSHFRLVLSQAFNSLGTVVAPYLAART
                     LLQGGLFEDGPVTEAKIAYSLGRIDVAYVFIAAVIAVLAVFLYRVRHEITAAAPPPEK
                     SASITSAFASPWALAGALSIFLYVGAEVAIGSAMVNFLEQPDVFAISAVEAGSFVSLY
                     WLGAMIGRFIGSGLLYKLPAGPLLAVAAVAAALLCLTISQISGPAAGVLAISVGLFNS
                     IMFPVIFSLTIERSTAPASATSGLLCMAIVGGALVPLAFAQVA"
BASE COUNT          227 a          586 c          563 g          327 t
ORIGIN      
        1 ctgatcctcg gcgccgagcc cggcgcgacc atcgcgctcg ggacgagggc gccggtcgac
       61 agggagaggc tgcgcgcggc cgcgctcgac ggcagcatcg aagaattgct cgactggaag
      121 cccgtcaaag cgggtgattt cttctatgtc ccctcgggca ccgttcatgc gatcggggcg
      181 gggctgacgc tgatcgaagt gcagcagaac agcgagacaa cctatcggct ctacgattac
      241 ggccgcccgc gcgagctgca tctcgatgac ggcatcgcgg tcgccgaccc gcggccctat
      301 acagcctatc cctcgccggg tcgcgttgcc gacgatcgcg agattctcgt cgacgggccg
      361 aagttcgtgc tcgaacgcct cgccggcgcg acgcggacgg tcgcgctccc cgatggcgtt
      421 accgcatggc tgatcccggt cgcgggcaac gggctcgccg acggcgtcaa cttcgcagcg
      481 ggcgaatgcc tcgcgctcac cggcacggtg cagatcgcgg ccgacgcggg cgcggacctg
      541 ttgctggcct atccgggaga ggggcgcctg ccatgaccgc tggccgatcg ggcacgggct
      601 ggacctttgc ctatgtcacc atgttgttct tcgtctgggg cgcggtcacc gcggtcaacg
      661 atatcctgat cccggcggtg aaggcgatct tcgcgctcag cgacaccgaa agctttctca
      721 cccaattcgc ctttttcatg gcctatggcg tcgtctcgct ccccgcggcg gcgctgatgg
      781 ggcggctcgg cgcggccaat tcgatcatcg tggcgctcgc gacgatgatc gtcggctgcc
      841 tgatcatgcc gctcgcgacc gtggtgcgcg aatatgcgat ggtgctcgtc ggcctgttcg
      901 tgatcgcctc gggcatcacg ctgttgcagg tcgctgccaa tccgctgtcg gcgtcgctcg
      961 gccgccccga acgctcgcat ttccggctgg tcttgagtca ggcgttcaat tcgctcggca
     1021 ccgtcgtcgc gccctatctc gccgcgcgca cgttgcttca gggcggactg ttcgaagacg
     1081 ggccggtgac cgaggcgaag atcgcctatt cgctcggccg gatcgacgtc gcctatgtct
     1141 tcatcgcggc ggtgatcgcg gtgctcgcgg tcttcctcta ccgcgtccgg cacgagatca
     1201 ccgccgccgc gccgccaccc gaaaaaagcg cgagcatcac gagcgccttt gcgtcgccct
     1261 gggcgctcgc gggggcgctg tcgatctttc tctatgtcgg cgccgaggtc gcgatcggca
     1321 gcgcgatggt caatttcctc gaacagcccg atgtattcgc gatcagcgcg gtcgaggcag
     1381 gcagcttcgt cagcctctat tggctcggcg cgatgatcgg ccgctttatc ggctcgggtc
     1441 tcctctacaa actacccgca gggccgctgc tcgcggtggc ggctgtcgcc gcggcgctgc
     1501 tctgcctgac gatcagccag atcagcgggc ccgcggcggg cgtgctcgcc atctcggtcg
     1561 ggctgttcaa ctcgatcatg ttccccgtga tcttctcgct gacgatcgag cgttcgaccg
     1621 cgcccgcgtc ggcgacctcg gggctgctct gcatggcgat cgttggcggc gcgctcgtgc
     1681 cgctcgcctt cgcgcaggtc gcc
//
LOCUS       DATMSU010000307         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_5753, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000307 DATMSU010000000
VERSION     DATMSU010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5753"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..591)
                     /locus_tag="VN034_08495"
     CDS             complement(199..591)
                     /locus_tag="VN034_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60677.1"
                     /translation="MKRFGGVLLTLIVLASCDRAPDNGDLAEAEARGSREAAANNGRI
                     ECALEGAKLFDRTCTVDEMSGADGTTLVVGRTNVGYRRLQITTDGRGVVSADGAEPAK
                     VSIVGDNMIEVAIGHDRYRLPANTGGAK"
     gene            complement(588..2438)
                     /gene="ilvD"
                     /locus_tag="VN034_08500"
     CDS             complement(588..2438)
                     /gene="ilvD"
                     /locus_tag="VN034_08500"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWV60678.1"
                     /translation="MPSYRSRTTTHGRNMAGARGLWRATGMKDSDFGKPIIAVVNSFT
                     QFVPGHVHLKDLGQMVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAALRINIPVVFVSGGPMEAGKVVLKGKEV
                     ALDLVDAMVAAADEKYSDEEVEEIERAACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSTLATHADRKELFLRAGRIVVEMCRRHYEEDDDSVLPRNIATFEAFENAMSLDIAMG
                     GSTNTVLHLLAAAFEAGVDFTMTDIDRLSRRVPCLSKVAPAKSDVHMEDVHRAGGIMA
                     ILGQLERAGLLHAHLPTVHSATLGDALNKWDISRTNDPDVQKFFMAAPGGVPTQTAFS
                     QDRRWDSLDLDREGGVIRSADHAFSKDGGLAVLSGNIALDGCIVKTAGVDESILKFSG
                     PAKVYESQDAAVAGILTGQVVAGDVIVIRYEGPKGGPGMQEMLYPTSYLKSKGLGAAC
                     ALVTDGRFSGGTSGLSIGHVSPEAAEGGTIGLVENGDLINIDIPSRTITLAVADSVLA
                     ERRAAMDAAGWQPEKARPRKVSVALQAYAAMTTSAARGAVRDLSQLKGKG"
     gene            complement(2498..>3018)
                     /locus_tag="VN034_08505"
     CDS             complement(2498..>3018)
                     /locus_tag="VN034_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWV60679.1"
                     /translation="SRLVVDCNREEDAPGVLPIASDGHAVPGNALDDEAREVRLARFF
                     RPYHDHIAATIAAARPAMILSLHSFTPSLAAHPDQARPWHVGVLYNEDDRLAGAAIAA
                     LEAEGLIVGDQQPYSGKLLNATMNRHAEANVIPYVGIEMRQDLVGETAGQALFAERLA
                     RMCEKVTLNIGA"
BASE COUNT          487 a         1056 c          929 g          546 t
ORIGIN      
        1 ggatcaggat cggcgcgccc gccgccatcc gccaggcggt atcggcaacg gccgcgcccg
       61 cgagttccat cagttccgaa agcgaggttc cctgcaccgc gcacgccgcc tcggcctcgc
      121 gcatcgcatc ggcggtcagg atcggggcgt cggcgggaac ggacatgggc cggccctacc
      181 acgatcgcgc ggacgcgcct acttcgcgcc gccggtattg gcgggcaggc gataacggtc
      241 gtggccgatc gcgacctcga tcatattgtc gccgacgata ctgaccttcg ccggttcggc
      301 gccatcggcc gacacgaccc cgcgcccgtc ggtggtgatc tgcaacctgc ggtaaccgac
      361 attggtgcgc ccgacgacga gggtcgtccc gtccgcgccg ctcatttcat cgacggtgca
      421 ggtgcggtcg aacagtttcg cgccttcgag cgcgcattcg atacggccat tgttcgcggc
      481 cgcctcgcgc gaaccgcgcg cctcggcttc ggcgagatcg ccattgtccg gcgcgcgatc
      541 gcagctcgcg agcacgatca gcgtcagcaa tactccccca aatcgcttca tcccttgccc
      601 ttcagctgcg acaggtcgcg caccgctcct cttgcggcgc tcgtcgtcat cgcggcatag
      661 gcctgaagtg ctaccgaaac cttgcgcggg cgcgccttct cgggctgcca ccccgccgcg
      721 tccatcgcgg cgcggcgctc ggcgaggacg ctgtcggcga cggccagcgt gatcgtccgc
      781 gatggaatgt cgatgttgat caggtcgcca ttctcgacca agcctatcgt gccgccctcg
      841 gccgcttcgg gcgaaacatg accgatcgac aggcccgagg tgccgcccga aaagcgcccg
      901 tcggtgacca gcgcgcacgc cgcgccaagc cccttggact tgaggtaact cgtcggatag
      961 agcatttcct gcattcccgg tccgcccttc gggccttcgt agcggatgac gatgacgtcg
     1021 cccgctacaa cctgtcctgt caggatgccc gcgaccgctg cgtcctggct ttcataaacc
     1081 ttggccggcc cggaaaactt caggatgctc tcgtccaccc ccgcggtctt cacgatgcag
     1141 ccgtcgagtg cgatattgcc cgacagcacc gcgagcccgc cgtccttgct gaacgcgtgg
     1201 tccgccgacc gaatcacgcc gccttcgcgg tcgaggtcga ggctgtccca gcgccggtcc
     1261 tggctgaacg cggtctgcgt cggcaccccg ccgggcgccg ccatgaagaa tttctgcaca
     1321 tcgggatcgt tggtgcgcga aatatcccat ttgttcagcg catcgcccag cgtggcgctg
     1381 tgaaccgtcg gcaggtgcgc gtggagcagc cccgcgcgct cgagttggcc gaggatcgcc
     1441 atgatcccgc ccgcgcggtg gacgtcctcc atatggacgt cgcttttcgc cggcgcgacc
     1501 ttcgacaggc acggtacgcg gcgcgacagc cggtcgatgt cggtcatggt gaaatcgacc
     1561 cccgcctcga aagcggcggc gagcaggtgg agcacggtat tggtcgaccc gcccatcgcg
     1621 atgtcgaggc tcatcgcatt ttcgaacgcc tcgaaggtcg cgatattgcg tggcaggacg
     1681 ctatcgtcat cctcctcgta atggcggcgg cacatttcga cgacgatccg ccccgcgcgc
     1741 aggaacaact ccttgcggtc ggcgtgcgtc gcgagcgtcg agccattgcc cggcaacgac
     1801 agccccaacg cctcggtcag gcagttcatc gagttcgcgg tgaacatgcc cgagcacgag
     1861 ccgcaggtcg ggcacgccgc gcgctcgatc tcctccactt cttcgtcgct atatttctcg
     1921 tcggcggcgg caaccatcgc gtcgacaaga tcgagcgcga cttccttacc tttcagcacc
     1981 accttgcctg cttccatcgg tccgcccgac acgaacacga cggggatgtt gatcctgagc
     2041 gccgccatca gcatgcccgg ggtgatcttg tcgcagttcg aaatgcacac catcgcgtcg
     2101 gcgcaatgcg cgttgaccat atattcgacg ctgtcggcga tcaggtcgcg gctcggcagc
     2161 gaatagagca tcccgtcgtg tcccatcgcg atcccgtcgt cgaccgcgat cgtgttgaat
     2221 tccttggcga cgccgcccgc cgcctcgatc tcgcgcgcga ccatctggcc gaggtctttc
     2281 aggtgaacat ggccgggcac gaactgggtg aagctgttga ccaccgcgat gatcggcttg
     2341 ccgaagtcgc tgtccttcat ccccgtcgcg cgccagaggc cgcgggcacc ggccatattg
     2401 cggccgtggg tcgtggtgcg cgaacgataa gaaggcattt ggagagttcc tgataatatt
     2461 atttcataaa atgacgatga aatgcgcctc tacacgccta tgcgccgatg ttcaacgtca
     2521 ctttctcgca catccgcgcg agccgctcgg cgaacagcgc ctgtcccgcc gtctcgccga
     2581 ccagatcctg gcgcatctcg atgcccacat agggaatgac attcgcttcg gcatggcggt
     2641 tcatcgtcgc gttgagcagc ttgcccgaat agggttgttg atcgccgacg atcagccctt
     2701 cggcctccaa agccgcgatc gccgcgccgg cgagccggtc gtcctcatta tagagcaccc
     2761 cgacgtgcca cggccgcgct tgatcgggat gcgccgcgag cgagggcgtg aaactgtgca
     2821 gcgacaggat catcgcgggc cgcgccgccg cgatcgttgc cgcgatatgg tcgtgatagg
     2881 gccggaagaa gcgcgccagt cgcacctcgc gcgcctcgtc atccagcgca ttgcccggga
     2941 ccgcatggcc gtcgctcgcg atcggcagca cccccggcgc atcctcctcg cggttgcagt
     3001 cgacgacgag ccgcgaca
//
LOCUS       DATMSU010000308         5377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_33847, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000308 DATMSU010000000
VERSION     DATMSU010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5377
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33847"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VN034_08510"
     CDS             complement(<1..870)
                     /locus_tag="VN034_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60680.1"
                     /translation="MSKYTAHELNLLVRPDGIGIVTLYTGAIAIPLVQHAYYGLRAYN
                     DPSSLLMIQRCFYAILISSQLEEMPSSSLAPKAIKVHADLSQYTHLNSCVKGIRGTVK
                     ANLPLRLTERNAELRKNLQGIFDFVSNFSRVREVSGEIMSLWSCVARCLDRGGDDFDV
                     SGMRQGIATFLSGRTWSVFESSLLDASDAWSLWLGWYKYRFAGVADGDFPSFLWRHWE
                     KEVADLPLDVWDKSPNDVNKILSEIALRILDDFVEKEVGKDQNRKSITFDISDKIHIE
                     NIYDENFDNRSSKI"
     gene            1083..2132
                     /locus_tag="VN034_08515"
     CDS             1083..2132
                     /locus_tag="VN034_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWV60681.1"
                     /translation="MLQKFALIAGAATAALALSACQDQASSGGGARGYISAVGSSTVY
                     PFATAVGEKFAEATGNKTPKIDSTGTGGGFERFCAGVGGDTPDIANASRRMKKKEFDS
                     CAANGVKEIVEVQIGIDGIALGEAARGPGFELTEEDVYKALAANPYGKPNTAKTWKDV
                     NPALPAVAISVFGPPSTSGTYDAFKELILGTGCDANPQMKALKDSDKDKHEAVCTTLR
                     GAPYYVEQGENDNLIISKLDKNPTSLGIFGFSYLDANKDKIKAVPIQGVAPTYAAIAD
                     GSYPGSRPLYIYVKKAHVGVVPGLAEYVAEFLKGAADGGYLNAKGLIVSPKDIAATAA
                     ANGKNMTALDGAELK"
     gene            2266..3642
                     /gene="pstC"
                     /locus_tag="VN034_08520"
     CDS             2266..3642
                     /gene="pstC"
                     /locus_tag="VN034_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWV60682.1"
                     /translation="MTFNAAALLLLIAGLALIGWLAGRARSNLLAGRAGAKLHSRPQY
                     HGWYVALWLFAPAALFLAIWSSVSPALITNQVLTTEAAQSLPEFGFERGAILSDARNV
                     AQGRQADVRLPAAAPLVKPYADASHKYAWIGIAAMLALALAGGLYAFTRIRPDFRARS
                     RVEKIVMMLLLLASLVAILTTFGIVLSLLFESIRFFRLVSPFELLFGTTWAPTSGAPQ
                     PDTFGGIPLFWGTVLIGAIIAMIVAIPIGMMTAVYLTQYAAPTVRKWVKPCLEILAGV
                     PTVVYGYFAALTVAPALREFAVMLGIPNASTESALAAGIVMGVMIIPFVSSMADDSIN
                     AVPQAMRDGSLALGATPNETIRQVLIPAALPGVMGGILLAVSRAIGETMIVVMAAGLS
                     ANMTANPFASVTTVTAQIVKLLTGDQEFDSAKTLAAFALGLVLFLVTLLLNIVALRIV
                     KKYREAYE"
     gene            3635..4891
                     /gene="pstA"
                     /locus_tag="VN034_08525"
     CDS             3635..4891
                     /gene="pstA"
                     /locus_tag="VN034_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWV60683.1"
                     /translation="MNRDTAPTDWKGAVMQKRIAGRYAAERRFKLMGLGAVLLSGAFL
                     AFLLFVMVGNGARGFTYTHVAVPVDFKAMPLTVDKARLGDDDADQVIANAGLADIVAF
                     AADEALGDGGSALISENAWKEVRSAIKADPDLLDGETLFELPASSEVDIAAKEGAKGA
                     LGARVDALEKDGKLSTGIHWAFFKNADATDPAVAGIWGALKGSVLTIFIAFLIAFPTG
                     VLAALYLEEYAPKNRWTDLIEVSINNLAAVPSIIFGLLALAVFINWFGLCQASPLVGG
                     LTLALMTMPVIVIASRNAIKSVPPSIRDAALGVGASPVQVVFHHVLPLALPGILTGTI
                     IGMARALGETAPLLLVGMRAFIGDVPGGICSPSTVLPMQIFLWSDEVDRGFVEKTSAA
                     IIVLLIVLLSMNAFAIYLRNKFEKRW"
     gene            4894..>5377
                     /locus_tag="VN034_08530"
     CDS             4894..>5377
                     /locus_tag="VN034_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWV60684.1"
                     /translation="MTQEDLTITDPKMKAHGVNVFYGEKQAINDVSIDVGTDLVTAFI
                     GPSGCGKSTFLRSLNRMNDTVASARVTGQIELDGEDIYAPSMDVVQLRARVGMVFQKP
                     NPFPKSIYDNVGYGPRIHGLAPSKADLDVIVERALVRAGLWDEVKDRLGESGTALSGG
                     Q"
BASE COUNT         1041 a         1691 c         1610 g         1035 t
ORIGIN      
        1 aattttgcta gacctattgt caaaattctc atcatatata ttttctatat gaattttgtc
       61 agaaatatcg aaagttatcg attttctatt ctgatccttt ccgacttctt tttcgacgaa
      121 atcatccaat atcctcaaag ctatctcgct gagaatttta tttacgtcat ttggactttt
      181 gtcccataca tcaaggggaa gatcagcaac ctccttctcc cagtggcgcc acaagaaaga
      241 aggaaaatcg ccatccgcca ccccagcaaa tcgatatttg taccaaccga gccaaagtga
      301 ccacgcatca gatgcgtcca acaagctgga ctcgaacact gaccaagtgc ggccagataa
      361 aaatgtcgcg atcccttgcc gcatgccgct tacgtcaaag tcgtcaccac cgcgatccaa
      421 acaacgagca acgcacgacc aaagcgacat tatctcgcca gaaacttctc taacgcgaga
      481 aaaattgctg acaaagtcga agataccttg gagattcttt cttagttctg cgtttctctc
      541 ggttagccgc agcggcaaat tcgcctttac ggtcccccta atccccttaa cgcacgagtt
      601 taaatgagtg tactgactta gatccgcatg aaccttaata gctttcgggg ccaaagagct
      661 ggatggcatc tcttctaact gactagatat tagaattgcg tagaagcaac gctgtatcat
      721 gagcaagctt gaaggatcat tgtaagcccg caggccatag taggcgtgct gaaccaacgg
      781 aattgcgatc gcaccagtat ataacgttac gataccaatt ccatctgggc gaactaataa
      841 atttagttcg tgagcggtat acttactcat tccgatagca ccatcgagtg catataaacc
      901 tccccggcgt cgagattcgc actaacgatt tttgacatca agtctaggag cagtcactgc
      961 acgtgacact tatatgacaa tccctgtcac aaacgcgtca cacaaaaccc ccattggcgg
     1021 ctcagcgagt cgccgcgggg gtggtggctt ggttcggtaa cgacccccga caggattatc
     1081 cgatgctgca gaaattcgcc cttatcgctg gcgccgccac agccgcgctg gcgctgtccg
     1141 cgtgccagga tcaggcgtct tcgggcggcg gtgcgcgcgg ttatatcagc gcggtcgggt
     1201 cttcgaccgt ctatcccttt gcgaccgcgg tcggcgagaa gttcgccgaa gcgacgggca
     1261 acaagacgcc caagatcgac agcacgggca ccggcggcgg cttcgagcgt ttctgcgccg
     1321 gcgtcggcgg cgacacgccc gacatcgcga acgcgtcgcg ccgcatgaag aagaaggaat
     1381 tcgacagctg cgccgcgaac ggcgtgaaag agattgtcga agtccagatc ggcatcgacg
     1441 gcatcgcgct cggcgaagcg gcgcgcggtc cgggtttcga gctgaccgag gaagatgtct
     1501 acaaggcgct ggcggcgaac ccctatggca agcccaacac cgcgaagacg tggaaggacg
     1561 tgaatcccgc gcttcccgcg gtcgcgatct cggtctttgg cccgccgtcg acgagcggca
     1621 cttatgatgc gttcaaggag ctgatcctcg gcacgggctg cgacgccaac ccgcaaatga
     1681 aggcgctgaa ggacagcgac aaggacaagc atgaagcggt gtgcacgacg ctgcgcggcg
     1741 cgccctatta tgtcgagcag ggcgagaatg ataatttgat catctcgaag ctcgacaaga
     1801 acccgacgag cctcggcatc tttggtttca gctatctcga cgccaacaag gacaagatca
     1861 aggcggtacc gatccagggc gtcgcgccga cctatgcggc gatcgccgac ggcagctatc
     1921 ccggctcgcg cccgctctac atctatgtga agaaagcgca tgtcggcgtc gtccccggcc
     1981 tcgccgaata tgtcgccgag tttctgaagg gcgcggccga cggcggttat ctgaacgcca
     2041 aggggctgat cgtgtcgccg aaggacattg cggcgaccgc ggcggcgaac ggcaagaata
     2101 tgaccgcgct ggatggtgcg gagctgaagt aagacggaca gaatagcctc cccctcccgc
     2161 ttgtgctgag cctgtcgaag caccgttctt ctcttcaaga caagaacggc ccttcgacag
     2221 gctcagggcg aacggacttg gggatagcgg aatgatgaga aaaacgtgac cttcaacgcc
     2281 gccgcccttt tgcttttgat cgcaggactc gccctgatcg gctggctcgc cggccgcgcg
     2341 cggtcgaacc tgctcgcggg ccgcgcgggg gcgaagctgc attcgcggcc gcaatatcat
     2401 ggctggtatg tcgcgctgtg gctattcgcg cccgccgcgc tgttccttgc aatctggtcg
     2461 tcggtgtcgc ccgcgctgat caccaaccag gtgctgacca ccgaggcggc gcagagcctg
     2521 cccgaattcg gcttcgagcg cggcgcgata ttgtccgatg cgcgcaatgt cgcgcagggg
     2581 cggcaggccg acgtgcggct gcccgctgcg gcgccgctgg tgaagcccta tgccgacgcg
     2641 agccacaaat atgcgtggat cggcatcgcg gcgatgctcg cgctcgcgct cgcgggcggg
     2701 ctttacgcct tcacgcgcat ccgccccgac ttccgcgcgc gaagccgcgt cgaaaagatc
     2761 gtgatgatgc tgttgctgct cgcgtcgctc gtcgcgatcc tgacgacctt cggcatcgtg
     2821 ctgtcgctgc tgttcgaatc gatccgcttc ttccgcctcg tctcgccttt cgagctcctg
     2881 ttcggcacga cctgggcgcc gaccagcggc gcgccgcagc ccgatacctt cggcggcatc
     2941 ccgctgttct ggggcacggt gctgatcggc gcgatcatcg cgatgatcgt cgcgatcccg
     3001 atcgggatga tgaccgcggt ctatctcacc caatatgccg cgccgacggt gcgcaaatgg
     3061 gtgaagccgt gcctcgagat cctcgcgggc gtgccgaccg tcgtctacgg ctatttcgcc
     3121 gcgctgaccg tcgccccggc gctgcgcgaa ttcgcggtga tgctcggcat cccgaacgcg
     3181 agcaccgaaa gcgcgctcgc cgcggggatc gtgatggggg tgatgatcat ccccttcgtc
     3241 tcctcgatgg ccgacgacag catcaacgcg gtgccgcagg cgatgcgcga cgggtcgctg
     3301 gcactcggcg cgacgccgaa cgagacgatc cggcaggtgc tgatccccgc cgcgcttccg
     3361 ggcgtgatgg gcggcatatt gctcgcggtc agccgtgcga tcggcgaaac gatgatcgtc
     3421 gtgatggcgg cgggcctgtc ggccaacatg accgccaacc ccttcgccag cgtcaccacg
     3481 gtgaccgcgc agatcgtgaa attgctcacc ggcgaccagg aattcgacag cgccaagacg
     3541 ctcgccgcct tcgcgctcgg gctcgtgctt ttcctcgtca cgctgctgct caacatcgtc
     3601 gcgctgcgca tcgtcaaaaa gtatcgcgaa gcttatgaat agagacaccg cccccaccga
     3661 ctggaaaggc gccgtgatgc aaaagcgcat cgcgggccgt tatgccgccg aacgccgctt
     3721 caagctgatg gggctgggcg cggtgctgct ctcgggcgcc ttccttgcct tcctgctctt
     3781 cgtgatggtc ggcaacggtg cgcgcggctt tacctatacg catgtcgcgg tgccggtcga
     3841 tttcaaagcg atgccgctga cggtcgacaa ggcgcggctc ggcgacgacg acgccgatca
     3901 ggtgatcgcg aatgcggggc tggccgacat cgtcgccttt gccgccgacg aggcgctggg
     3961 cgacggcggg tcggcgctga ttagcgagaa tgcgtggaag gaagtgcgca gcgcgatcaa
     4021 ggccgatccc gacttgctcg acggcgagac gcttttcgag ctgcccgcgt cgagcgaggt
     4081 cgatatcgcc gccaaggaag gcgccaaggg cgcgctcggc gcaagggtcg acgcgctgga
     4141 gaaggacggc aagctgtcga ccggcatcca ctgggccttc ttcaagaatg ccgacgccac
     4201 cgatcccgcg gtcgcgggca tctggggcgc gctcaaggga tcggtgctga cgatcttcat
     4261 cgccttcctc atcgctttcc cgaccggcgt gctcgcggca ctctatcttg aggaatatgc
     4321 gccaaagaac cgctggaccg acctgatcga ggtgtcgatc aacaacctcg ccgcggtgcc
     4381 ctcgatcatc ttcggcctgc tcgcgctcgc ggtgttcatc aactggttcg gcctctgtca
     4441 ggcgagcccg ctcgtcggcg ggctgacgct cgcgctgatg acgatgccgg tgatcgtgat
     4501 cgccagccgc aatgcgatca agtcggtgcc gccatcgatc cgcgacgccg cgctgggtgt
     4561 cggcgccagc ccggtgcagg tggtgttcca ccatgtgctg ccgctcgccc tccccggcat
     4621 cctcaccggc accatcatcg gcatggcgcg tgcgctgggc gagacggcgc cgctgctgct
     4681 cgtcggcatg cgcgccttta tcggcgacgt cccgggcggc atctgttcgc cttcgaccgt
     4741 gctgccgatg cagatattcc tctggtcgga cgaagtcgac cggggctttg tcgagaaaac
     4801 ctccgccgcg atcatcgtgc tgcttatcgt gctcctgtcg atgaacgcct ttgcgatcta
     4861 tcttcgcaac aaattcgaaa aacgctggtg aacatgaccc aagaagacct gaccattacc
     4921 gacccgaaga tgaaggcgca cggcgtcaac gtcttctatg gcgagaaaca ggcgatcaac
     4981 gacgtgtcga tcgacgtcgg caccgacctg gtcaccgcct tcatcggccc gtcgggctgc
     5041 ggcaagtcga ccttcctgcg ctcgctgaac cggatgaacg ataccgtcgc cagcgcgcgc
     5101 gtcaccgggc agatcgaact cgacggcgag gatatctatg cgccgtcgat ggacgtcgtg
     5161 cagttgcgcg cccgcgtcgg catggtgttc cagaaaccca accccttccc caagtcgatc
     5221 tatgacaatg tcggctatgg tccgcgcatc cacggcctcg cgccgtcgaa ggccgacctc
     5281 gacgtcatcg tcgagcgcgc gctggtgcgt gcgggcctgt gggacgaggt caaggaccgg
     5341 ctcggcgaaa gcggcaccgc gctgtccggc ggccagc
//
LOCUS       DATMSU010000309         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_24069, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000309 DATMSU010000000
VERSION     DATMSU010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24069"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VN034_08535"
     CDS             <1..607
                     /locus_tag="VN034_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV60685.1"
                     /translation="DEYMWTFNMPTDHPRMTFSDVWVPDSARLGPEGGGLAIAQSFVH
                     QNRIRQAASSLGAAVYCIEESVKYARTRKPFGEALAKNQAIQFPLVELATQAEMLRLL
                     IRKTAWDMDNTPHKEIERTLSDKVSMCNYWANRLVCEAADRAMQVHGGIGYSRHKPFE
                     HIYRHHRRYRITEGAEEIQMRKVAAFLFGYMGPRKETLGKI"
     gene            complement(828..1577)
                     /locus_tag="VN034_08540"
     CDS             complement(828..1577)
                     /locus_tag="VN034_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWV60686.1"
                     /translation="MIRSLTLALLLAVSATPLAAKETAAPDYKAALADPARPAADRER
                     DAARKPADLLAFAQIAPGEKVGDFVMGGGYVTRLLAAAVGPSGKVYAFQPAEFIKFKA
                     QYGTDQTAVDAAYPNVDAVAGPFAAPAFTTPLDTIVTVQNFHDLYLKPFPEGTGAKAS
                     AALFAALKPGGTLVVVDHSAADGSGTTLSDSLHRIDKAAVVAALTKAGFKLEAESDLY
                     KRADDPRTANVFDAGIRGKTDQFALRFRKPG"
     gene            1701..3440
                     /locus_tag="VN034_08545"
     CDS             1701..3440
                     /locus_tag="VN034_08545"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HWV60687.1"
                     /translation="MHDLVIRGGTVVDGSGGAPFVADVAVDGDRIVAVGENLGAGAKE
                     IDASGKIVTPGFVDVHTHYDGQATWDAEMAPSSWHGVTTVVMGNCGVGFAPAKPDRHE
                     WLISLMEGVEDIPGTALAEGMSWDWETFPEYMDALEKLPRTVDVACHVPHGAVRAYVL
                     GDREKPGAIPTDADIAEMSRIVEEGVRAGALGFSTSRTVLHKSVDGELVPGTTATAEE
                     LIAIGRAMGRVGYGVFEMASDLKREWNEFQWMGDLSRETGLPVTFAALQSIAKELPLE
                     EQIEEMRRQNASGANIVAQIALRGNGVIMAWQGTVHPFRFKPAWNEIIDLPWEQQLAK
                     LKDPAFKARMIEEENIWPESDIIDFLKVVALGWPVQFEMDPDFNYEPKMDESIMARAA
                     AAGVSPSEYAYELLMKDDGKGFIYFPILNYRDGNLNFLEDLQASDDTVNSLSDGGAHC
                     GTICDAASPTFMLQHWVRDRKGHRIALEHAVKRQCRDTALLYGLEDRGILAPGYLADL
                     NVIDMDAIKLGKPWLAFDLPAGGKRLLQKADGYVATVKSGVVTFRDGTMQGPTPGGVI
                     RGPQRVEMAMAAE"
     gene            complement(3457..>4473)
                     /locus_tag="VN034_08550"
     CDS             complement(3457..>4473)
                     /locus_tag="VN034_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60688.1"
                     /translation="PLERRRATFLRAQLRAAETRLQMMQGTRFAFADEAERLFGVRPR
                     LKPLASYDAELAKIEKLVPGEGALWARVEAYLDAFTIPKDRLQKTFDAAIARCRGRSM
                     AHIPMPEGESFRLEFVTGKSWSGYNYYQGGYHSLIQVNTDLPIRLSRALDLGCHEGYP
                     GHHLLNMKLEEKLVKERGWSEFSVYPLYSPQSLIAEGSANYGIDLAFPEGSKADTERD
                     VLMPIAEIAVPPDDRYWQLLKAIKRASGARLTIAQQYLDGEIDRATAIALTQKYQLVS
                     PQRAEQSISFTDQYRSYVINYGLGETIVRAYVERGRPSRDEMWRRMAKIVSEPTLPSD
                     LLAR"
BASE COUNT          798 a         1391 c         1505 g          779 t
ORIGIN      
        1 cgatgaatat atgtggacgt tcaacatgcc gaccgaccat ccgcgcatga cgttcagcga
       61 tgtgtgggtg cccgacagcg cgcggctcgg ccccgagggc ggcgggctcg cgatcgcgca
      121 gagcttcgtc caccagaacc gcatccgcca agcggccagt tcgctgggcg cggcggtcta
      181 ctgcatcgag gaaagcgtca aatacgcgcg cacccgcaag ccgttcggcg aggcgctagc
      241 gaagaaccag gcgatccagt tcccgctcgt cgagctcgcg acgcaggccg agatgctgcg
      301 cctcctgatc cgcaagaccg cgtgggacat ggacaacacc ccgcacaagg aaatcgagcg
      361 aacgctctcc gacaaggtca gcatgtgcaa ctattgggcg aaccgcctcg tctgcgaagc
      421 cgccgaccgc gcgatgcagg ttcacggcgg catcggctac tcgcgtcaca aacccttcga
      481 gcatatctac cgccaccacc gcagataccg catcaccgaa ggcgccgagg aaatacaaat
      541 gcgcaaagtc gccgcattcc tgttcggata tatggggcca aggaaagaaa cgttggggaa
      601 aatctgaggc tggatgttgg ttaggggtgg atttaagcca ttcccctccc ttttagggag
      661 gggttagggg tgggtgcgcg agcgtagcga gccagaaaag agcgccgcct tcggcggcta
      721 cccacccccg gcccctccct aaaagggagg ggagaaggta gggccgcttc gagccgtttt
      781 ctgacattcc ggcccggcgc gttatgcgat cggaccagcg tttcggttca tcccggcttc
      841 ctgaaccgca gcgcgaactg gtcggtcttg ccgcgaatgc ccgcatcgaa gacgttggcg
      901 gtgcgcgggt cgtcggcgcg cttgtagaga tcgctttccg cctcgagctt gaagcccgcc
      961 ttggtgagcg cggcgacgac cgcagccttg tcgatgcggt gcaggctgtc ggacagcgtc
     1021 gtgccgctgc cgtcggcggc gctatgatcg acgacgacga gcgtgccgcc gggtttcagc
     1081 gccgcgaata gcgcggcgct cgccttggca cccgtgcctt cgggaaaggg tttgaggtag
     1141 aggtcgtgga aattctgcac cgtgacgatc gtgtcgagcg gtgtcgtgaa tgcgggcgcc
     1201 gcgaacgggc ccgccaccgc gtcgacattc ggataggcgg catcgaccgc ggtctgatcg
     1261 gtgccatatt gcgctttgaa cttgatgaac tcggcgggct ggaaggcata gaccttgccc
     1321 gacgggccga ccgcggcggc gagcaagcgg gtgacgtaac cgccgcccat cacgaaatcg
     1381 ccgacctttt cgcccggcgc gatctgcgcg aaggcgagca gatcggcggg tttgcgcgcg
     1441 gcgtctcgtt cgcggtcggc ggcggggcgc gccggatcgg cgagtgcggc tttatagtcg
     1501 ggggcagctg tctccttcgc cgcaagcggc gtggccgaaa cggcgaggag gagggcgagg
     1561 gtaagcgagc ggatcatggc aacgactccc ttcatatggt cctgccacca tagcgtctta
     1621 ccgcgacggc ggcgtgctta tttgcatctc gcgattttga tgataggttt ttgtgtgaaa
     1681 ctaaaaatcg ggagacggac atgcatgatc tggtgattcg cggcggcacc gtcgtcgatg
     1741 gttcgggcgg cgcgcccttt gtcgccgacg tcgcggttga cggtgatcgc atcgtcgcgg
     1801 tcggcgaaaa tctgggcgcg ggcgccaagg aaatcgacgc gagcggcaag atcgtcaccc
     1861 ccggcttcgt cgacgtccac acccattatg acgggcaagc gacgtgggac gccgaaatgg
     1921 cgccgtcgag ctggcacggc gtcaccaccg tcgtcatggg caattgcggc gtcggcttcg
     1981 cccccgccaa gcccgaccgc cacgaatggc tgatttcgct gatggaaggc gtcgaggaca
     2041 tccccggcac cgcgctcgcc gagggcatgt cgtgggactg ggaaaccttt cccgaatata
     2101 tggacgcgct cgaaaagctg ccgcgcaccg tcgacgtcgc gtgccatgtc ccgcacggcg
     2161 cggtgcgcgc ctatgtgctc ggcgaccgcg aaaagcccgg cgcgatcccg accgacgcgg
     2221 acatcgccga aatgtcgcgc atcgtcgagg aaggtgtgcg cgcgggtgcg ctcggcttct
     2281 cgaccagccg caccgtgctc cacaagtcgg tcgacggcga gctcgtcccc ggcaccaccg
     2341 cgaccgccga ggaactgatc gcgatcggcc gcgcgatggg gcgcgtcggt tacggcgtgt
     2401 tcgaaatggc gagcgacctg aagcgcgaat ggaacgagtt ccagtggatg ggcgacctca
     2461 gccgcgaaac cgggctgccg gtgaccttcg ccgcgctcca gtcgatcgcc aaggaattgc
     2521 cgctcgagga gcagatcgag gaaatgcggc gccagaatgc gagcggcgcg aacatcgtcg
     2581 cgcagatcgc gctgcgcggc aacggcgtca tcatggcgtg gcagggcacc gtccatccct
     2641 tccgcttcaa gcctgcgtgg aacgagatca tcgacctgcc gtgggagcag cagctcgcga
     2701 agctgaagga cccggcgttc aaggcgcgga tgatcgaaga ggaaaatatc tggcccgaaa
     2761 gcgatatcat cgacttcctc aaggtcgtcg cgctgggttg gccggtgcag ttcgaaatgg
     2821 accccgactt caattacgag ccaaagatgg acgaaagcat catggcgcgc gcggccgctg
     2881 cgggggtgag cccgtccgaa tatgcctatg agctgctgat gaaggacgat ggcaagggct
     2941 tcatctattt cccgatcctc aactaccgcg acggcaacct caatttcctc gaggatttgc
     3001 aggcatcgga cgacacggtg aacagcctct ccgacggcgg cgcgcattgc gggacgatct
     3061 gcgacgccgc ttcgccgacc ttcatgctcc agcattgggt gcgcgaccgc aagggccacc
     3121 gcatcgcgct cgaacatgcg gtgaagcgcc agtgccgcga cacggccttg ctctatgggc
     3181 tcgaggatcg cggcatactc gcgccgggct atctcgccga tttgaacgtc atcgacatgg
     3241 acgcgatcaa gctcggcaag ccgtggctcg ccttcgatct gcccgcgggc ggcaagcgtc
     3301 tgctgcaaaa ggccgacggt tatgtcgcga cggtcaagtc gggtgtcgtg accttcaggg
     3361 atggcaccat gcagggcccc acccccggcg gcgtgatccg cggcccgcag cgcgtcgaaa
     3421 tggcgatggc ggcggagtaa tccgccgcct cgcgcgtcac cgtgcgagca aatccgacgg
     3481 caacgtcggt tcgctgacga tcttcgccat ccgccgccac atctcgtcgc ggctcggccg
     3541 gccgcgctcg acataggcgc gcacaatcgt ctcgccgagg ccgtagttga tcacatagct
     3601 gcgatactgg tcggtgaagc tgatcgattg ttcggcgcgc tggggcgaca cgagctgata
     3661 tttctgcgtc agcgcgatcg cggtggcgcg gtcgatctcg ccgtcgagat attgctgcgc
     3721 gatcgtcagc cgcgcgcctg acgcacgctt gatcgctttc agaagctgcc agtaacggtc
     3781 atcgggcggc accgcgatct cggcgatcgg catcagcacg tcgcgttcgg tgtcggcctt
     3841 gctgccctcg gggaaggcga ggtcgatgcc gtagttggcg ctgccttcgg cgatcaggct
     3901 ttgcgggctg taaagcggat agacgctgaa ctcgctccag ccgcgctcct tcaccagctt
     3961 ttcctcgagc ttcatgttga ggagatgatg gccgggataa ccctcatggc agccgagatc
     4021 gagcgcgcgc gacaggcgga tcggcaggtc ggtgttgacc tggatcaggc tgtgatagcc
     4081 gccctgataa taattatagc cgctccagct tttgccggtg acgaattcga ggcgaaagct
     4141 ctcgccttcg ggcatcggga tatgcgccat gctgcgtccg cggcaacgcg cgatcgcggc
     4201 gtcgaaggtc ttttgcaggc ggtccttggg gatggtgaag gcgtcgagat aggcctcgac
     4261 gcgcgcccag agcgcgcctt cgcccggcac cagcttttcg atcttcgcga gttcggcgtc
     4321 gtagctcgcg agcggcttga ggcggggccg cacgccgaac agtcgctcgg cctcgtcggc
     4381 gaaggcgaag cgcgtgccct gcatcatctg caatcgcgtc tcggcggcgc gaagctgcgc
     4441 gcgcaggaag gtcgcgcggc ggcgttcgag cgg
//
LOCUS       DATMSU010000310         5185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_81070, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000310 DATMSU010000000
VERSION     DATMSU010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81070"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VN034_08555"
     CDS             complement(<1..703)
                     /locus_tag="VN034_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60689.1"
                     /translation="MTEKDRAGTSGGAAGTSGLSARALTALLLAAMLLFGIVRITANV
                     YSYIADKQAAGLEVSQTTAWMLEGTALAAWVVMSVPLWFTVRAIRPPRFGLAATIAIH
                     ALLVIPVALGHVAVMVALRTILWWFTGGSYQFGAPDGDPILYEFRKNVSTYAELVFIL
                     FLVQWLVARYAAPPVPAAERRTLAIGDGPVTHHLPVDEIEHVGAAGNYVEVAWRGERL
                     LHRATLTAIETELAGA"
     gene            796..1941
                     /locus_tag="VN034_08560"
     CDS             796..1941
                     /locus_tag="VN034_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWV60690.1"
                     /translation="MQAARHYGMDWLRIGAFALLILYHIGMVFVPDHWHVKSDPTIDW
                     VAIPMFATNSWRLPLLFLVSGYASAALVSKLGGAGAFATSRSARLLIPLVFAVVVVIP
                     SQPWIELVGQHGYDHGFPHFWAHDYFRFGSLGGIILPTWQHLWFVVYLWVYTMLAALF
                     LAALPAAARARIADGAARLLSGWGLLIVPMLGWLAVYAAFPGHEETHALFDDGPSHLH
                     YLMAFGVGWLLRVRPALFESVARCWKAAAVLAILAFLPVAWTEWTWPGDTHAPEWAII
                     LFHVARQVQGWATIVALVGIADRWWNRDHPKRAMLAEAVFPFYIVHQTIIVVVGWWLL
                     QAGVAALPSFVILVAATVSGCWLFYAIGRSIGWLRPLIGLQRKTGRV"
     gene            1941..2138
                     /locus_tag="VN034_08565"
     CDS             1941..2138
                     /locus_tag="VN034_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60691.1"
                     /translation="MGALKGILAIVGVAMILAGGLWALQGLDIVRWPAESFMLGDTTW
                     TRNGVVLAVAGVVLVWFARKR"
     gene            complement(2157..2639)
                     /locus_tag="VN034_08570"
     CDS             complement(2157..2639)
                     /locus_tag="VN034_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV60692.1"
                     /translation="MTLAIRSATPADLPLIAGFIRDLAEYEKLSHEVRFDEAKLGDKL
                     FGPRPYAEVVIGEIDGSPQGFALFFHNFSTFEGRPGIYLEDLFVRPEARGSGLGKALL
                     AHLAKLCVERDCARLEWWVLDWNTPSIGFYQSLGAKPMDEWTVMRVDGDALTKLAAAS
                     "
     gene            complement(2671..3087)
                     /locus_tag="VN034_08575"
     CDS             complement(2671..3087)
                     /locus_tag="VN034_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60693.1"
                     /translation="MPAKPDTLDRLVQLLLLIAAVTTIANGLFMVAKPLDWYVFVPTV
                     VTTGPPNAHFIRDIGLAYLGSGLILLYAAANPAPRWRAMIVGGLWLTFHGLLHIYEVA
                     AGICGPATFWADAPAVLGQPALVIAALAILKARSRI"
     gene            complement(3352..4503)
                     /gene="ispG"
                     /locus_tag="VN034_08580"
                     /gene_synonym="gcpE"
     CDS             complement(3352..4503)
                     /gene="ispG"
                     /locus_tag="VN034_08580"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWV60694.1"
                     /translation="MSDHNPGLRPWRDIARRECRQIMVGNVPVGGGVPISVQTMTNTL
                     TSDPVATIDQIRRCEEAGADLIRVSCPDTDSTAALGKIVRAARIPIIADIHFHYKRAL
                     EAADAGAACLRINPGNIGSSERVGEVVRAAKANGCAIRIGVNAGSLEKDLLEKYGEPC
                     PEALVESALDHIKLLQDHDFHEYKVAVKASDVFLAVAAYAQLADAVDCPLHLGITEAG
                     GLIGGTVKSALGIGNLLWAGIGDTIRVSLSAEPEEEVRVGYEILKSLGLRTRGVRVVS
                     CPSCARQGFDVIRTVQALEEALQHIKTPMSLSVLGCVVNGPGEARETDIGITGGGNGK
                     HMVFLSGVTDHHVEDADMIQHIVGLVEAKAAEIEAGSAVSMDVTHGKAA"
     gene            4760..>5185
                     /locus_tag="VN034_08585"
     CDS             4760..>5185
                     /locus_tag="VN034_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HWV60695.1"
                     /translation="MKFFGKLCFGMLLGASAMSTPAFAQDEAAETEASGPLSLSGGIS
                     VTSDYRFRGISLSNEKVAVQPTLTLSHDSGFYIGTWGSSLPDSPAYGKFELDLYGGYA
                     TEIAPGTSIDVGVTWYTYPGSDDGGAPTDYFEAIGKLSHD"
BASE COUNT          812 a         1574 c         1835 g          964 t
ORIGIN      
        1 ccgcgccggc gagttcggtc tcgatcgcgg tcagcgtcgc gcggtggagc aggcgctcgc
       61 cacgccatgc gacctcgaca taatttcccg ccgcgccgac atgctcgatc tcgtcgaccg
      121 gcaggtgatg cgtgaccggc ccgtcgccta tcgcgagcgt cctgcgttcg gccgccggca
      181 cgggcggcgc ggcgtagcgg gcgacgagcc actggacgag gaagaggatg aagacgagtt
      241 cggcataggt cgagacgttc ttgcggaact cgtagagaat gggatcgcca tcgggcgcgc
      301 cgaattgata gctgcccccg gtgaaccacc agaggatcgt gcgcagcgcc accatgacgg
      361 cgacatggcc aagtgcgacg ggaatgacga gcagcgcgtg gatcgcgatg gtggcggcaa
      421 ggccgaagcg cggcgggcgg atcgcgcgga cggtgaacca gagcgggacg ctcatcacca
      481 cccatgcggc gagcgcggtg ccttcgagca tccatgcggt ggtctgcgaa acctcgagcc
      541 ccgccgcctg cttgtcggcg atataggagt agacgttcgc ggtgatgcgg acgatgccga
      601 acagcagcat cgcggcgagc agcagtgcgg tcagcgcgcg cgcgctcaaa ccgctcgtac
      661 ccgctgcgcc cccgctcgtc ccggctctgt ctttctccgt cacatcggcc ccgctgttcg
      721 tcccgccggg atcgcccgcc gtccgccttt ccgattagcg gctgcggcaa gtcccggcaa
      781 aggagatttg ggcagatgca agcggcgcgg cattacggaa tggactggct caggatcggg
      841 gcgttcgcgc tgctgatcct ctatcatatc ggcatggttt tcgtgcccga tcattggcat
      901 gtgaagagcg acccgacgat cgactgggtc gcgatcccga tgttcgcgac gaacagctgg
      961 cggctgcctt tgctgttcct cgtctcgggc tatgcgagcg cggcgttggt gtcgaagctc
     1021 ggcggagcgg gggcgttcgc gacatcgcgg agcgcgcggc tgttgattcc gctcgtcttc
     1081 gcggtcgttg tcgtcatccc gtcgcagccg tggatcgaac tcgtcggcca gcatggctat
     1141 gatcatggct tcccgcattt ctgggcgcat gactatttcc gcttcggctc gctcggcggg
     1201 atcatcctgc cgacctggca gcatctgtgg ttcgtcgtct atctgtgggt ctatacgatg
     1261 ctcgcggcgc tgttcttggc agcactgccc gcggcggcgc gggcgcggat cgccgacggc
     1321 gcggcgcggc tgctgtcggg ctgggggctg ctgatcgtcc cgatgctcgg atggcttgcg
     1381 gtctatgcgg cgtttccggg gcatgaggag acgcacgcgc tgttcgacga cggaccttcg
     1441 cacctccatt atctgatggc cttcggggtc ggctggctgc tgcgcgtgcg gccggcgctg
     1501 ttcgagagcg tcgcgcgctg ctggaaggcg gcggcggtgc tcgccatact cgccttcctg
     1561 ccggtcgcgt ggaccgaatg gacatggccc ggcgatacgc atgctcccga gtgggcgatc
     1621 atcctgttcc atgtcgcgcg gcaagtgcag gggtgggcga cgatcgtcgc gctcgtcggg
     1681 atcgccgacc gctggtggaa ccgcgatcat ccgaagcgcg cgatgctcgc cgaggcggtc
     1741 ttcccctttt acatcgtcca ccagacgatc atcgtcgtcg tcggctggtg gctgcttcag
     1801 gcgggtgtcg cggcgttgcc ctctttcgtg atcctcgtcg cggccacggt gagcgggtgc
     1861 tggcttttct atgcgatcgg gcgcagcatc ggctggctgc ggccgctgat cggactgcag
     1921 cgaaaaacgg ggagggtttg atgggcgctt tgaagggcat attggcgatc gtcggggtgg
     1981 cgatgatcct cgccggcgga ttgtgggcgc tgcaggggct cgacatcgtc cgctggcccg
     2041 cggagagctt catgctcggc gacacgacat ggacgcgcaa tggcgtcgtc cttgcggtcg
     2101 ccggcgtggt gctggtctgg ttcgcgcgga aacggtaaaa atcgcccggc gaatggtcag
     2161 gaagcggcgg caagcttcgt cagcgcatcg ccgtcgacac gcatgaccgt ccattcgtcc
     2221 atcggcttgg cgccaaggct ctggtaaaag ccgatcgacg gggtgttcca gtcgagcacc
     2281 caccattcga ggcgcgcgca atcgcgctcc acgcaaagct tggcgagatg cgcgagcagc
     2341 gccttgccga gccccgagcc gcgcgcttcg gggcgcacga agagatcctc gagatagatg
     2401 ccgggtcgcc cctcgaaggt cgagaaattg tggaagaaga gcgcgaagcc ctgtgggctc
     2461 ccatcgatct cgccgatcac aacctcggca tagggacgcg ggccgaagag tttgtcgccg
     2521 agcttcgcct cgtcgaagcg gacttcgtgc gacagctttt catattcggc gaggtcgcgg
     2581 atgaacccgg cgatcagcgg caggtcggcg ggggtggcgg agcggatggc gagggtcatg
     2641 ggcgtgctgt gtcggcgcct gcgtccgctg tcaaatgcgc gatcgcgctt tcaatatcgc
     2701 gagcgccgcg atgacgagcg cgggctggcc gagcaccgcc ggcgcatcgg cccagaaggt
     2761 cgcggggccg caaatcccgg cggcgacctc gtagatgtgg agcagcccgt ggaaggtcag
     2821 ccagagaccg ccgacgatca ttgcgcgcca gcggggggcc gggttcgcgg cggcatagag
     2881 gaggatcagg cccgagccca gataggcgag tccgatgtcg cggatgaaat gcgcgttggg
     2941 cggcccggtg gtgacgacgg tgggcacgaa aacataccag tcgagcggct ttgccaccat
     3001 gaagaggcca ttggcgatcg tcgtgaccgc ggcgatcagc aacagaagct ggacgaggcg
     3061 gtcgagggtg tcgggcttgg cgggcatggc gggtcgctcc tgctggggag ggtgaggcta
     3121 gccgttactg acagcgatgt aaaaggggtg tggggtgggg ggttggttcc ggccgggttg
     3181 cggacgttat ggggatggct ctctcccctt caggggagag atacaaaggc ttggcagctt
     3241 gctgcctagc cgcagtagag aggggctggg ctatgtcccc ctctcccaac cctctcccct
     3301 gaaggggaga gggctatacg cccgctcctc accccaaagc ggaaaccggc tctacgccgc
     3361 cttgccgtgc gtcacgtcca tgctcaccgc gctacccgcc tctatctccg ccgccttggc
     3421 ttcgacgagc ccgacgatgt gctggatcat gtcggcgtct tcgacatggt ggtcggtgac
     3481 ccccgacagg aagaccatat gtttgccgtt gccgccgccg gtgatgccga tgtcggtttc
     3541 gcgcgcctcg ccggggccgt tgacgacgca gcccaatacc gacagcgaca tcggggtctt
     3601 gatgtgctgg agcgcctctt cgagcgcctg gacggtgcgg atcacgtcga agccctgccg
     3661 cgcgcagctg gggcaggaga cgacgcggac gccgcgggtg cggaggccga gcgacttcag
     3721 gatttcatag ccgacgcgca cttcttcttc gggctcggcc gagaggctga cgcggatcgt
     3781 gtcgccgatg ccggcccaga gcagattgcc gatgccgagc gccgacttga cggtgccgcc
     3841 gatgaggccg ccggcttcgg tgatgccgag gtgcaaggga caatcgaccg cgtcggcgag
     3901 ctgcgcatag gcggcgaccg cgaggaagac gtcgctcgcc ttcaccgcga ccttatattc
     3961 gtggaagtcg tggtcctgga gcagcttgat atggtcgagc gcgctttcga cgagcgcctc
     4021 ggggcagggc tcgccatatt tttcgagcag gtctttctcg aggctgccgg cgttgacgcc
     4081 gatgcggatc gcgcagccgt tggccttggc ggcgcggacg acttcgccga cgcgctcgct
     4141 gctgccgatg ttgccggggt tgatgcgcag gcacgcggcg ccggcgtcgg cggcttccaa
     4201 tgcgcgctta tagtggaaat gaatgtcggc gatgatcggg atgcgcgccg cgcggacgat
     4261 cttgccgagc gccgcggtcg aatcggtatc ggggcacgag acgcggatca ggtcggcgcc
     4321 cgcctcctcg cagcggcgga tctggtcgat cgtcgcgacc ggatcgctgg tcagcgtgtt
     4381 cgtcatcgtc tggacgctga tcggcacgcc gccgccgacg gggacgttgc cgaccatgat
     4441 ctggcggcat tcgcgccgtg caatgtcgcg ccaggggcgc aggccgggat tatggtcgct
     4501 catagatggg gctcgggcaa aagatggtat cggccgctct ttagccgccc gggcgcggct
     4561 tggcaaagcg aagcacatgg cgcggcactt gttgcgtgaa agacacagtt tgacacagat
     4621 cggcaatttt tgggcaattt ttatttgtga gtccgccccg aatgcgcaat tttggtgaag
     4681 ttcgtcgtca caggcccgga taaggaggcg tggacacgcg cagcaggcgg gacgatccgt
     4741 tttttgaggg ggatcaccca tgaagttttt tggcaagctg tgtttcggca tgctgctcgg
     4801 cgcgtcggcg atgtcgacgc cggctttcgc gcaggacgaa gcagcggaaa cggaagccag
     4861 cggcccgctc tcgctttcgg gcggcatttc ggtcacctcc gactatcgtt tccgcggcat
     4921 ttcgctgtcg aacgagaagg ttgcggtgca gccgacgctg acgctcagtc acgacagcgg
     4981 tttttatatc ggcacctggg gatcgtcgct tcccgacagc cccgcctatg gcaagttcga
     5041 actcgacctt tatggcggct atgcgaccga aattgcgccg ggcacgtcga tcgacgtcgg
     5101 cgttacctgg tacacctatc cgggcagcga cgatggcggc gccccgaccg actatttcga
     5161 ggcgatcggc aagctgtcgc acgac
//
LOCUS       DATMSU010000311         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_71809, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000311 DATMSU010000000
VERSION     DATMSU010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71809"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2271
                     /locus_tag="VN034_08590"
     CDS             <1..>2271
                     /locus_tag="VN034_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60696.1"
                     /translation="EAAMAAAGAEAGDAGETGETGEIVVTGMKFDRTLQDTPESVKVF
                     TAEEIDRQSLVNVYDLIDRTANLSSSFAESGFNIRGISNNNVSGYGFGDLATVYLDGS
                     PLPGEAAGGGPLDLWDLEQVEILRGPQSTLQGRNALAGAIILKTADPSFDWRGKARAM
                     ITDANGEYRFAAAVGGPIVDDVLAFRVAGEIARGDGLVTNRLTGGDYDRTNSEVVRTK
                     LLFTPAGADGLQVKLSYLYDRHFGNKGRGYVFSDVPDAFDNRFVEANRPTFTRTSTDL
                     FTADLSLPLGEVFTLSSVSTYNHLKTFSTYDGELRAEDEAYGDFATNQKSFSQEVRLN
                     FDTGPLQGLIGGYYSRLKNPRSDSNSTFSLDPDADLGLTPQVAAILQSQFGLPADQAL
                     FLAQQARQLYPPRIFIASDQRYSIDVETMALFGDASFEVAPGLKLLAGFRYDHERQKV
                     ANDNVVVLNTVLPDPADYASDPLLAGIILTVNGVLQQQVDNANSSGPQSTTTYKAFLP
                     KGGVSWEVAPDKTLSAIVQRGYRSGGSGVNPGRATTHVYDPEYTWNYELSLRTQWLDR
                     RLTFNANAFYIDWKDQQVYVQLSDNVYDYETQNAGSSRVWGFEVESDFDVTERLNIYG
                     SVGYANSKFLDFQTSNGQVLDLSGFEFANAPRWTWAAGFDWRHPSGFSANLNVNYRGA
                     QFQDIRDQSGGRDVPARTLVNAKVGYGNDHFGAYLVATNIFDDEYYDYQYENGGRMQA
                     LFGEPRMIGLTFEGRF"
BASE COUNT          412 a          723 c          736 g          400 t
ORIGIN      
        1 gggaggccgc gatggcggcg gcgggggccg aagccggcga tgcgggcgag acgggcgaga
       61 cgggcgagat cgtcgtcacc ggcatgaagt tcgaccgcac gttgcaggac acgcccgaaa
      121 gcgtgaaggt gttcaccgcc gaagagatcg accggcagtc gctggtcaat gtctatgacc
      181 tcatcgaccg caccgcgaac ctgtcctcct ccttcgccga atcgggtttc aacatccgcg
      241 gcatttcgaa caataatgtc tcgggctatg gcttcggcga ccttgcgacc gtctatctcg
      301 acgggtcgcc gctgccgggc gaagcggcgg gcggcgggcc gctcgacctg tgggacctcg
      361 agcaggtcga gatattgcgc gggccgcaat cgaccttgca ggggcgcaac gcgctcgccg
      421 gcgcgatcat cctcaagacc gccgacccga gcttcgactg gcgcggcaag gcgcgcgcga
      481 tgatcaccga tgcgaacggc gaatatcgct tcgccgcggc ggtcggcggg ccgatcgtcg
      541 acgatgtgct ggcctttcgc gtcgcgggcg agatcgcgcg cggcgacggg ctggtcacca
      601 accggctgac cggcggcgat tacgaccgga cgaacagcga ggtcgtgcgc accaagctgc
      661 tcttcacccc ggcgggcgcc gacgggttgc aggtcaagct ctcctatctt tacgaccggc
      721 atttcggcaa caaggggcgc ggctatgtgt tcagcgacgt gcccgacgcc ttcgacaatc
      781 gtttcgtcga ggcgaaccgg ccgaccttca cccgtacgag caccgacctg ttcaccgccg
      841 atctgtcgct gccgctcggc gaggtcttca ccctgtcgtc ggtgagcacc tataatcacc
      901 tcaagacctt ctcgacctat gacggcgagc tgcgcgccga ggatgaagcc tatggcgatt
      961 tcgcgaccaa ccagaagagc ttcagccagg aagtgcggct gaacttcgac accgggccgc
     1021 tgcaggggct gatcggcggc tattattcgc ggctgaagaa cccgcggtcg gattcgaaca
     1081 gcacgttcag cctcgacccc gacgccgatc tggggctgac gccgcaggtc gcggcgatcc
     1141 tccagagcca gttcgggctg cccgccgatc aggcgctctt cctcgcgcag caggcgcgcc
     1201 agctctatcc gccgcgcatc ttcatcgcgt cggaccagcg ctattcgatc gacgtcgaga
     1261 cgatggcgct gttcggcgac gcgagcttcg aggtcgcgcc ggggctgaag ctgctcgcgg
     1321 gcttccgcta cgatcatgag cggcagaagg tcgcgaacga caatgtcgtc gtgctcaaca
     1381 ccgtgctgcc cgatcccgcc gactatgcgt ccgatccgtt gctcgcgggg attatcctga
     1441 cggtgaacgg cgtgctgcag cagcaggtcg acaatgcgaa cagcagcggc ccgcagtcga
     1501 cgacgaccta caaggcgttc ctgcccaagg gcggggtttc atgggaggtc gcgcccgaca
     1561 agacattgag cgcgatcgtc cagcgcggct atcgttcggg gggcagcggg gtgaaccccg
     1621 ggcgcgcgac gacgcatgtc tatgaccccg aatatacgtg gaattacgag ctgtcgctgc
     1681 gcacccaatg gctcgaccgc cggctgacct tcaacgccaa cgccttctat atcgactgga
     1741 aggaccagca ggtctacgtc cagctttccg acaacgtcta tgattatgag acgcagaatg
     1801 cgggcagttc gcgcgtctgg ggtttcgagg tcgagagcga tttcgacgtc accgagcggc
     1861 tgaacatcta tggttcggtc ggctatgcga acagcaagtt cctcgatttc cagaccagca
     1921 acggccaggt acttgatctg tcgggcttcg agttcgccaa cgcgccgcgc tggacctggg
     1981 ccgcgggctt cgactggcgc catccgtcgg ggttcagcgc caacctcaac gtcaattacc
     2041 gcggtgcgca gtttcaggac atccgcgacc agagcggcgg ccgcgacgtg ccggcgcgca
     2101 cgctcgtcaa cgccaaggta ggttatggca atgaccattt cggcgcctat ctggtcgcga
     2161 cgaacatctt cgacgacgaa tattatgatt atcaatatga gaatggcggc cggatgcagg
     2221 cgctgttcgg cgaaccgcgg atgatcgggc tgaccttcga agggcgtttc t
//
LOCUS       DATMSU010000312        18258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_185388,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000312 DATMSU010000000
VERSION     DATMSU010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 18258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18258
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185388"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VN034_08595"
     CDS             <1..316
                     /locus_tag="VN034_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWV60697.1"
                     /translation="LDIDWSSLARGGSTLAFYMGREAAGEIRRGLMQAGMPGEMPVMI
                     ACEVSGPGERRLVTRLDLLDLATKSFAADAPTLILVGAAVMLAGSAPALPANSSRATY
                     GR"
     gene            complement(351..1058)
                     /locus_tag="VN034_08600"
     CDS             complement(351..1058)
                     /locus_tag="VN034_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWV60698.1"
                     /translation="MSGMIDPGARGSLVERAVESVRDFIRVHDLKVGDTLPGEGSFAQ
                     QLGVSRPVMREAFGALAALRLVDVGNGRKPRVGAIDGSVMAASMGHAVNTAQVSLADV
                     WEVRRTLELRTAELAAINRTDEQARAILAAAHGLSVGHDEAARTAADTVFHQTIAEAS
                     GNQLFYQIVRSFEQLMAVAIPRAWAGRTTHEERDETLALHREVAEAIADRDPARARAA
                     MESHFNNSIGEMLKDQF"
     gene            complement(1055..1804)
                     /locus_tag="VN034_08605"
     CDS             complement(1055..1804)
                     /locus_tag="VN034_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWV60699.1"
                     /translation="MPPKHPVIVSPEMTTYVGRGSCDRLLLPPDALLLGFGDIGEPVV
                     RTLTFRELENEEHLLVFAVSRGACRRLFGDLPDPDARWYLPSDLRALGQSIVAPDSDE
                     GATDTLRLARSIELLCQFFAALRAGRLVPVEGQPSLTESDIARITAARQIVDTRWQEK
                     LTVDDIARSCGINRDKLTRGFRELYQCSVAEALSERRLRQARQMLAASDLPVASIGYR
                     CGYLNNAAFTRAFSRRFGMAPTAMRRVGIAA"
     gene            complement(1856..3163)
                     /locus_tag="VN034_08610"
     CDS             complement(1856..3163)
                     /locus_tag="VN034_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60700.1"
                     /translation="MATHAESLAVPTQRLTARATLVPLLLLALVTTMGFTALGSFGTI
                     QESAKAELALSDDALALIQGLGAAVPMVLFSIPIGILVDRRNRVRLTIGLALLWTLGT
                     LLTAFAPSAELLTAARMLVGIGSTGSLTAALSLCADYCAPEQRGRAMLIVNLGKALGV
                     ALGFALTGWLFGLLADFSAPGWLAGLAPWRGAHVLLAAISALCLLPLLFLREPVRREV
                     EASPDAPFRIVAAELWARRAFLGPLFAGQVAVVMADVAATVWVAPVLSRDFGLQPQQF
                     AGWVGALMFGTGVAGAVLGGLSADMGQRSRRRGGILLGAVIAAGVGVPAAMFPLMPSV
                     AGLAVALGVMSLCGAVTGLVVSVALTVLLPNELRGLCIGAFIAIAGLIGFGLAPWVVT
                     RVSALLGGEAMLAEALALVGVTVSALSFFAFLLAMRRAPEPVR"
     gene            complement(3163..4566)
                     /locus_tag="VN034_08615"
     CDS             complement(3163..4566)
                     /locus_tag="VN034_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWV60701.1"
                     /translation="MAGTAHSGERKPLPASLVAALEKRFGDRFQRGQAVLGQHGSSET
                     HFAPVLPDAVVFAKSTDDVVALVKLCSAADIPIVPFGAGTSIEGNALAINGGISLDMS
                     LMDRVIAVNAEDFDCVVQPGVRREELNVHLRDQGLFFPIDPGANATIGGMASTRASGT
                     NAVRYGTMKDAVLSLEIVTPQGDVIRTARRARKSAAGYDLTRLYVGSEGTLGIITEVT
                     LRLHPVPDTISAAVCSFDALGGAVDTVVQSIQCAVPLARVEILDTKQMIAVNRWSKLD
                     YPEAPTLFFEFHGSAANVAEQVETVKSLAEANGGSAFSWSNLPEERTKLWRARHEAYY
                     AAVNMRAGAIGWATDVCVPMSRLAECIAETHVDLDAASVPATILGHVGDGNFHVIFSI
                     DPDAPEEMAEVEAINARLVERALAMDGTCTGEHGIGIGKQDWLVAELGGAVEQMRMIK
                     RALDPQNLLNPGKIFAL"
     gene            complement(4569..6710)
                     /locus_tag="VN034_08620"
     CDS             complement(4569..6710)
                     /locus_tag="VN034_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60702.1"
                     /translation="MTKFRFAAVSTLVLAAAFGATPVFAQDSAATPAAAEGEDDGAIV
                     VTARKREETLNDVPIAATAITGDTLIARGINSVREAAVLSPGLNINSDGTGRAFVSIR
                     GVGVTLVQSVQPGVGLFIDGIYQPNTAYLNNPLLDVERIEVLRGPQGTLYGKNTMGGA
                     INVITRQPGNDFEVRANASYAGPDDAWMVSGSVSGPIVTDKVALRIAAAHRQQDGFLK
                     NSVIGGNANPFNTDSVNATLRVTPSDDFTLTIKGYYDWVDGVNTPYSRVTGPKDYSRI
                     VQFNTLNAVSYKYRGINVRAEGDLGGGSKLSVIGAYDMRNGFTPDGEGDFGPSDIVRT
                     VARDSLRTMTLESRLDSEWSDQISTLFGLFYSNEMVQAQARDTISLVLPDIGPVSIVR
                     NTRAKNVADTYAAFGTVFWKPNLDWEVALGLRYDHEDRVANGSVSGAIVPTARIKSNE
                     WQPKLSVTRKWTPDLMTYVSVARGYRGGGFNAPTAPTRTYRGDSAWTYEAGAKYSSPV
                     FNLSGSVFYSDYKDYIGLNSIAPAAGGGLVTVDLNTGDVESYGVELEAMVRPVRNWTL
                     RGGLTWMHARITDDSAYTATTGRQLSSDRLTFQPDWTASFASDYRIETGGDSDVTLSA
                     GLVGKGKRLAATLNETTPTFLDSYWLTNASIAYRTGPVEVALFANNIFNTEYFESYIE
                     RTTLALAGLPASDLGITGDRRRYGVRASLKF"
     gene            complement(6829..7602)
                     /locus_tag="VN034_08625"
     CDS             complement(6829..7602)
                     /locus_tag="VN034_08625"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWV60703.1"
                     /translation="MYLDKLRLDGRTAFVTGGAQGIGLATAEALAEAGAKVTIADRDP
                     AALDRAVAELSAKGYAIFAAELDVTHSAAVDAAADAMLAREGRIDILVNNAVIARSET
                     AAEDVADEHWLNVIDVNLNGSFWCARAFGRHMLAAGQGSIVNVGSMSGFIVNRPQPQS
                     YYNASKAAVHQLTKSLAAEWAGRGVRVNAVAPTYISTPLNAFADKQGDMYRRWIDGTP
                     QARLGEPEEVASVILFLASDMASLMTGSVVLADGGYSCW"
     gene            complement(7607..9091)
                     /locus_tag="VN034_08630"
     CDS             complement(7607..9091)
                     /locus_tag="VN034_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWV60704.1"
                     /translation="MIYEQHSASIETEAGAIRGRALGSVIRYLGVPYAAPPTRFELPE
                     PPRSWDGVRDMESPGPAAPHRIRPFPKVDPSPLVGHGSDGSDGDYLRLNVWAPKDAKA
                     APVMIFIHGGGFVGGCKDAPVHDGSAFAESGIVCVAINYRMGIDGFLPVPGAPTNLGL
                     RDMLFALAWVQRNIAVFGGDPANVTVFGESAGAMAIADLVTSPLATGLFRRAIIQSGH
                     GAMVREIDIAQRLVKKLAKILRVSPDADGFRAVSHAAAMDALDKVGKPWAVDLRDGEG
                     REPVYGISRFIPVYGDDVLPEKPLDALRKGAGRDVEILIGTNSEEMNLYFVPTGVRDK
                     IGGLFARWLLGRSHPQAKAVLRAYGYQQKGVRPGQAMTDAMDDLVFRWPARQYAEAHQ
                     GKTWMYEFDWRSPACDGELGACHGIEMPFVFKTLASVTGPRGLAGLDPPQALADAVHD
                     LWAGFARTGTMPWPEFGTYRMVYQVTRGEALHEPAMPAAAFVPE"
     gene            9338..9472
                     /locus_tag="VN034_08635"
     CDS             9338..9472
                     /locus_tag="VN034_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Entericidin EcnA/B family protein"
                     /protein_id="HWV60705.1"
                     /translation="MKKLLTIFALSGSIFILSACNTVEGAGKDVESAADCADGVEGNC
                     "
     gene            complement(9598..10758)
                     /locus_tag="VN034_08640"
     CDS             complement(9598..10758)
                     /locus_tag="VN034_08640"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HWV60706.1"
                     /translation="MIAPAERKPNWLRGKAGKIRKRWKILATIVVLLALLSQCSITKS
                     LVESTRKPDAPFAQTKAPPAPDYAQNAAWFALPGANGLERSVPPGFAAIDERDAPADV
                     FFIHPTMYLKNDVWNAPYDVEGAYNGPVLLAQLSAFNGCCLLYAPHYRQASLAGLRDG
                     DAVAFAYDDVARAFRYYVEHYNRGRPFIIASHSQGTGHAVRLLQEAIIGTPLQDRLVA
                     AYTVGAYTPASFASLGLPTCDDAHQTGCVLSWNASQEGRTAASMLTKDVDFWWQGKRV
                     KSTAPAICVNPLTWNEKGIANAASNPGSLPFPEAPYPRQAATLAPLEKGLTGARCTDM
                     LLDVDVAWGSPFRDTLSWLYGSYHRNDYGLFYASIRANAIDRVASWQARHPD"
     gene            complement(10755..11462)
                     /locus_tag="VN034_08645"
     CDS             complement(10755..11462)
                     /locus_tag="VN034_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV60707.1"
                     /translation="MTKAVKQTNRRRPTKADQRAATMEQIYDAAEELFSKRGLYGVTL
                     KEVAAQVGVHQSLLHYYFKDKKELFDAVIARRASVTVERRMKALDAYERAVEGKPTVE
                     GALRAFLDTDLELFSEGGIGWRHYGQLGAQMSNTAEWGAELMDTHFDHVVLRLIGIIK
                     QAMPDCPEEDLFWGYHFVSAGLMLTLGRTGRIDKLSGGLCLSDDFEAVKERMARFMAA
                     GFIATCEARRAAREGGK"
     gene            complement(11516..13096)
                     /locus_tag="VN034_08650"
     CDS             complement(11516..13096)
                     /locus_tag="VN034_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWV60708.1"
                     /translation="MISGNPQPYALTLDKFLRHAAKWHGQREIVTGAAPARADYAGLC
                     ERSNRLSGAFAALGLREGDHLATLAWNTQAHMECWYAAMGAGIVCHTLNPRLTADHLA
                     KMMHRAGNRVLAVSSGLAPLVQDLVGQCPAIEHVVLMDDGPASDAVPVVTGARLWHLA
                     DLLAEHGAPVLWGAFDENAPAGLCFTSGTTGDPKGVTYTHRSNYLHTTHLLQADVMGL
                     TCEDSVLVAVPMFHANGWGFPFAAPAVGAKLVLPGRDQDGASLAKLIVDEEVTVAAGV
                     ATVWLGLVDHLDAVGGELPSLERLLLGGSAVPQALMERLERRLGVTVQTSWGMTELSP
                     LGAMTPATAKTRVAAKSGRPPMGVDLLLTDAHGTPLTDQRGGEGRLWVKGGSVVERYF
                     GHGEKATGPNGWFDTGDLATIDADGNLSITGRAKDLIKSGGEWINPAEIEAIVGSLPE
                     VALVAVVARKHEKWGERPVLIVEPRAGAEVSDETLLDALRGRVANWWLPDAIVRRTAM
                     PLALTGKIDKMRLRADHG"
     gene            complement(13153..14460)
                     /locus_tag="VN034_08655"
     CDS             complement(13153..14460)
                     /locus_tag="VN034_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60709.1"
                     /translation="MPAASSPSVSPAGRQGDRYGWFVLAALCFVYVLNFLDRQLLSIL
                     AKPIQDDLGITDGELGLLGGLYFAMFYCVLGIPVAWLADRTNRVRVLSVACALWSAAT
                     IACGMSTTYVQLAAARMGVGIGEAGGVPPSYSIISDYFPPERRGLALGLFNLGPPLGQ
                     ALGVAFGAKIAAAYDWRLAFLLLGVAGLIAAAGVWAFVREPRRGATDVVRAVEPAGER
                     ATFGATMKMFFARADLTLVSLAAGATQFVTYATLGFTTLFLMREKGMTLDEIAIWYAL
                     VLGIGVSAGIYLSGRLVDRYAAERPEAYGLIPAISLLLAVPFFIGFVHTPAWPLAMLF
                     LLGPTFLNYFYLTPAVTLVQNAVPARQRTLAGAVLLLIMNLIGLGLGPTWLGAVSDWL
                     KPTHPDNSLQLAFYSLVPFYFVAIALHLALARVLKRKASRDAA"
     gene            complement(14527..16707)
                     /locus_tag="VN034_08660"
     CDS             complement(14527..16707)
                     /locus_tag="VN034_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60710.1"
                     /translation="MRKLLLSVSAVALLGPVPVFAQTAPVEEATADAFGDEIVVTAER
                     RTTSLQRSSVAATVLTGEDLVRKGVNSVEGLQFASPSLTVNTTGQGNAFNIRGIGKTE
                     QTSAIGVGVITYRDGVATLPGYFQSEPYYDIASVEVLRGPQGTFAGGNATGGAVFITQ
                     QNPTFDRVGGYFLAQYGNYDHMKLQGALNIPHSDTLAIRFAGNMERRDTFYDMSGPWT
                     GNPGNRRDYSARVSIAWEPTPELTVLLKGDYNNIEYGGLPASRATETGDPLDVASDAH
                     LSGRDEFGRVVLNVAYKFDSGLTLRSISGFQRGTTQISYDSDGTATIIPALSFRDNIS
                     EEIWSQEFNIVSPDTGPFTWVLGGYYQHDKITIPENGGYYSDATPNTPTALDIEIVGT
                     NPKTALAAFGQVGYELTDGLQVTIGGRWSRTTSANDAEARAYFTGIPGPILVLPQVDR
                     TVNKKVNGKVALNWTVDPNHFLYAFVATGSKAGGLNGPNILGRTPLAFDAEDVTDFEL
                     GWKGTFFDGHLRTQLGGYYNLYKNFQVSIVDPVSPSISSIFNVPGTTKLYGVEFSAQG
                     SFDRFSLDLAGSVSNSKLGTFFAADPRLGGAPAPCNPENGPATPRCLDLTGNEQAYAP
                     KLTLSAGAQYAIPLGGDATLTPRVDFAHIAASWGSLFQNRLFGDRLEARNILNAQLTL
                     TTGDWSIAAYGTNLTDQHYVAAINGARRLAGAPRQYGLRVSRNF"
     gene            complement(17008..>18258)
                     /locus_tag="VN034_08665"
     CDS             complement(17008..>18258)
                     /locus_tag="VN034_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HWV60711.1"
                     /translation="VPAGALAAGRQAPTGFLWGTAISAHQSEGGNTASDSWLLETNKP
                     TAFADPSGDACDSYHRYEEDLDIAASLGLNCYRFGIEWARIEPAEGMFSEAALDHYAR
                     VIEACRARGLMPMVTYNHFTVPLWFAMRGGFEVADGADLFARFCGRATERLGRLIGMA
                     TTFNEANILGLRKVIPRFASDAAAENARAMIAAAAKRTNSPKFSSMLFGDPDKIDKVM
                     LDAHAKGYAAIKAARSDLPVGVTLSMQEIQGIGEGHRGKEAEETMYGGWIEAARQSDF
                     VGVQTYSRLRIGPKGLLPPTSGAPLTDMGYENYPPAVGHTIRYAAKHIGKPLYLTETG
                     IGTTDDAARIAFIKATMDEVSRCIDDGIEVKGYLYWSLLDNFEWTSGYAKKFGLVEVD
                     RKTFKRTLKPSAHYLGMRAKANRI"
BASE COUNT         3257 a         5986 c         5914 g         3101 t
ORIGIN      
        1 gctcgacatc gactggtctt cgctggcgcg cggcggatcg accctcgcct tttacatggg
       61 ccgcgaggcc gcgggcgaaa tccggcgcgg cctgatgcag gcggggatgc cgggcgaaat
      121 gccggtgatg atcgcgtgcg aggtcagcgg ccccggcgaa cggcgactcg tcacgcggct
      181 cgatcttctc gaccttgcga ccaaatcctt cgccgccgac gccccgacgc tcattctggt
      241 cggggccgcg gtgatgctcg ccggcagcgc gccagccctg cccgcgaaca gctcgcgcgc
      301 cacctatggg cgatagcttt cgtcggcgac cgtcagcggc gcgaacaaga tcagaactga
      361 tccttgagca tctcgccgat cgaattgttg aaatggcttt ccatcgcggc gcgggcgcgc
      421 gcgggatcgc gatcggcgat cgcctcggcg acctcgcggt gaagtgcgag cgtttcgtcg
      481 cgttcctcgt gagtcgtgcg ccccgcccag gcgcgtggga tcgcgaccgc catcaactgc
      541 tcgaacgaac ggacgatctg gtaaaaaagc tgattgccgc tcgcctcggc gatcgtctgg
      601 tgaaagacgg tgtcggccgc ggtccgcgcc gcctcgtcat gcccgaccga caggccgtgc
      661 gcagcagcga ggattgcgcg cgcctgttcg tcggtgcgat tgattgcggc gagttcggcg
      721 gttcgcagtt cgagcgtgcg gcggacctcc cacacgtcgg cgaggctgac ctgcgcggtg
      781 ttgacggcat gccccatcga cgcggccatc accgatccgt cgatcgcgcc gacacgcggc
      841 ttgcgcccgt tgccgacgtc gacgaggcgc agcgcggcaa gcgcgccgaa cgcctcgcgc
      901 atcaccggcc ggctgacgcc gagttgttgt gcaaaactgc cctcgcccgg cagcgtgtca
      961 cccaccttga ggtcgtggac gcggatgaag tcgcgcaccg attcaaccgc gcgctcgacg
     1021 agcgagccgc gcgcgccggg gtcgatcatc ccactcatgc cgcgattccg acgcggcgca
     1081 tagcggtcgg cgccatgccg aagcggcgcg agaaggcgcg ggtaaaggca gcgttgttga
     1141 gatagccgca acggtatccg atgctcgcca ccggcaagtc gctcgctgcg agcatctgcc
     1201 tggcctggcg caagcgccgc tcgctcagcg cctcggcgac gctgcattga tagagttcgc
     1261 gaaacccgcg cgtcagctta tcgcgattga tcccgcagct tcgggctata tcgtcgaccg
     1321 tcaatttctc ttgccagcgg gtgtcgacga tctgccgcgc ggcggtgatc cgcgcgatgt
     1381 cgctttcggt gaggctcggc tgcccctcga ccgggaccag ccgcccggcg cgcagcgcgg
     1441 cgaagaactg acagagaagt tcgatgctgc gggcgaggcg tagtgtgtcg gtggcgcctt
     1501 cgtcgctgtc gggcgcgacg atcgactgtc cgagcgcgcg aaggtccgac ggaagatacc
     1561 agcgcgcatc ggggtcgggc agatcgccga acaggcggcg gcacgcgccg cgcgagacgg
     1621 cgaaaacgag cagatgctcc tcattttcca gctcgcgaaa ggtcagcgtg cggacgacgg
     1681 gttcgccgat gtcgccgaag ccgagcagca gcgcatcggg cggcaacaac agccgatcgc
     1741 acgaaccgcg gccgacatag gtcgtcattt cgggtgacac gatgacagga tgctttggcg
     1801 gcatgactct ctccctgcag ccgcccatag acctatatga tagctttcac aatagctatc
     1861 ttacaggttc gggcgcgcga cgcatggcca ggagaaaggc gaagaaggag agcgcgctga
     1921 cggtaacccc gacgagcgcg agcgcctccg ccagcatcgc ctcgccgccc aacaacgcgc
     1981 tcacacgcgt caccacccaa ggcgcgaggc cgaatccgat cagtccggcg atcgcgatga
     2041 aagcgccgat gcagaggccg cgcagttcgt tggggagcag cacggtgagc gcgaccgaca
     2101 ccacgaggcc tgtaacggcg ccgcaaagcg acatgacgcc caatgcaacc gcaagtccgg
     2161 ccacgctcgg catcagcggg aacatcgccg cgggaacccc cacccccgcc gcgatgaccg
     2221 cgcccagaag aatgccgccg cggcggcggc ttcgctgccc catatcagcc gaaagcccgc
     2281 caagcaccgc acccgcgacg ccggtgccga acatcagtgc gcctacccag cctgcgaatt
     2341 gctgcggctg gagtccgaaa tcgcgcgaca gcacgggcgc gacccagacg gtcgcagcaa
     2401 catcggccat cacgaccgcg acctgcccgg caaagagcgg gccgagaaag gcgcggcgcg
     2461 cccagagttc ggcggctacg atgcggaagg gcgcgtcagg actcgcctcg acctcgcgcc
     2521 gcaccggttc gcgcaggaac agcaagggga gcaggcagag cgcgctgatc gcagcgagca
     2581 gcacatgcgc gccgcgccag ggagcgagac cggcgagcca gccgggcgcc gaaaaatcgg
     2641 cgagcaagcc gaacagccag cccgtcagcg cgaagccgag cgcgacgccg agcgctttcc
     2701 ccagattgac gatcagcatc gcgcggccgc gctgttcggg cgcgcaataa tcagcgcaga
     2761 gcgacagcgc cgcggtgagc gaaccggtcg agccgatccc gacgagcatc cgcgccgcgg
     2821 tgagcaactc cgcgctcggc gcgaaggcgg tgagcaaggt gccgagggtc cagaggagcg
     2881 cgaggccgat cgtcagccgc acgcgattac ggcgatcgac gagaataccg atcgggatcg
     2941 agaagagcac catcggcacc gcggcgccga gcccctggat cagcgcgagg gcatcgtcgc
     3001 tgagcgccag ttcggccttg gcgctttcct ggatcgtgcc gaagctgccg agcgcggtga
     3061 agcccatcgt cgtcaccagc gcgagcagca gcagcgggac gagcgttgcg cgcgcagtca
     3121 ggcgctgcgt cggcacggcg agcgattcgg cgtgcgtcgc catcacagcg cgaaaatctt
     3181 gccggggttg agcagattct gcggatcgag cgcgcgcttg atcattcgca tctgttcgac
     3241 cgcaccgccg agttcggcga cgagccagtc ctgcttgccg atgccgatgc catgttcgcc
     3301 ggtacaggtg ccgtccatcg cgagcgcgcg ttcgacgagc cgggcattga tcgcctcgac
     3361 ctccgccatt tcttcgggcg cgtcgggatc gatcgaaaag atgacgtgga aattgccgtc
     3421 gccgacatgg ccgaggatcg tcgcgggaac gctcgcggcg tcgaggtcga catgcgtttc
     3481 ggcgatgcac tcggcgagcc ggctcatcgg tacgcacaca tcggtcgccc agccgatcgc
     3541 gcccgcgcgc atattgaccg cggcataata ggcctcgtgc cgcgcgcgcc acagcttcgt
     3601 gcgctcctcg ggcaggttcg accagctgaa cgcgctgccg ccgttcgcct cggcgagcga
     3661 ctttaccgtt tcgacctgtt cggcgacatt ggctgcgctg ccgtggaatt cgaagaaaag
     3721 cgtcggcgcc tcgggataat ctagcttgga ccagcggttg acggcgatca tctgcttcgt
     3781 gtcgaggatc tcgacgcgcg ccagcggcac cgcgcactgg atcgactgga cgacggtatc
     3841 gaccgcgccg cccaacgcat caaagctgca caccgccgcc gaaatcgtgt cgggcaccgg
     3901 atgcagacgc agggtgactt cggtgatgat gccgagcgtg ccttcggagc ccacgtaaag
     3961 ccgggtaaga tcgtaacccg ccgccgactt gcgcgcgcgg cgcgcggtgc ggatcacgtc
     4021 gccctgcggc gtcactatct cgaggctgag caccgcgtcc ttcatcgtgc catagcgcac
     4081 ggcgttcgtc cccgacgcgc gcgtcgacgc catgccgccg atcgtcgcat tggcgccggg
     4141 atcgatcggg aagaacagac cctggtcgcg cagatggacg ttcagttcct cgcggcgaac
     4201 gcccggctgg acgacgcaat cgaaatcttc ggcgttcacc gcgatcaccc gatccatcag
     4261 gctcatgtcg agcgagatgc cgccgttgat cgccagcgca ttgccctcga tcgacgttcc
     4321 ggcgccgaac ggcacgatcg gaatatcggc ggccgagcag agcttcacca gcgcgacgac
     4381 atcgtcggtc gatttcgcga agacgacggc gtcgggcagc acgggagcga aatgggtctc
     4441 gctcgatcca tgctgcccga gtaccgcctg gccgcgctgg aaacggtccc cgaaccgctt
     4501 ctcgagcgcc gccaccaggc tggcgggaag cggcttgcgt tcccccgaat gcgccgtgcc
     4561 cgccatagtc agaatttcag gctcgcgcgc acgccgtaac ggcggcggtc cccggtgatg
     4621 ccgaggtcgg acgccggcag gcccgcgagc gcaagcgtcg tcctttcgat atagctttcg
     4681 aaatattcgg tgttgaagat gttgttcgcg aacagcgcga cctcgaccgg cccggtgcga
     4741 tacgcgatcg acgcgttggt cagccaatag ctgtcgagga aggtcggcgt cgtttcgttg
     4801 agcgtcgcgg ccaggcgctt gcccttgccg accagtcctg cgctcagtgt cacgtcgcta
     4861 tcgccgccgg tctcgatgcg ataatcgctc gcgaagctgg cggtccagtc gggctggaag
     4921 gtcaggcggt cggacgagag ctgacgcccg gtggtcgcgg tataggcgct gtcgtcggtg
     4981 atgcgcgcat gcatccacgt cagcccgccg cgcagcgtcc agttccgcac cgggcgcacc
     5041 atcgcttcga gttcgacgcc atagctttcg acgtcgccgg tgttgagatc gacggtgacg
     5101 agccccccgc cggcagcggg cgcgatcgaa ttgagtccga tataatcctt gtagtcgctg
     5161 taaaatacgc tgcccgacag attgaaaaca ggcgaggaat atttggcgcc cgcttcatag
     5221 gtccacgccg aatctccccg ataggtccgc gtcggcgcgg tcggcgcgtt gaagcccccg
     5281 ccgcgatagc cgcgcgcgac cgacacatag gtcatcaggt caggggtcca tttgcgcgtc
     5341 accgacagct tcggctgcca ttcgttcgac ttgatccgcg cggtgggcac gatcgcgccc
     5401 gataccgacc cgttggcgac ccggtcttca tggtcgtaac gcaggccgag cgcgacctcc
     5461 caatcaaggt tcggtttcca gaagacggtg ccgaaggcgg cataggtatc ggcgacattc
     5521 ttcgcgcgcg tattgcggac gatcgagacc ggtccgatgt cggggagcac gagcgaaatc
     5581 gtatcgcgcg cctgtgcctg caccatctcg ttgctgtaga aaaggccgaa cagcgtcgaa
     5641 atctggtccg accattcgct gtcgagccgc gattcgagcg tcatcgtgcg caggctgtcg
     5701 cgcgcgacgg tgcgcacgat gtcgctcggc ccgaaatctc cctcgccgtc gggggtgaag
     5761 ccgttgcgca tgtcataggc gccgatgacg ctcagcttcg acccgccgcc aagatcgccc
     5821 tcggcgcgca cgttgatgcc tcgatatttg taggagacgg cgttgagcgt gttgaattga
     5881 acgatgcgcg aataatcctt tggcccggtg acgcgcgaat agggcgtatt caccccgtcg
     5941 acccagtcat aatagccctt gatcgtcagc gtgaaatcgt cggacggcgt tacgcgcagc
     6001 gtcgcgttga ccgaatcggt gttgaacgga ttggcgttgc cgccgatcac gctgtttttc
     6061 aggaagccgt cctgctggcg gtgcgccgcg gcgatgcgaa gcgcgacctt gtcggtgacg
     6121 atcgggcccg aaaccgatcc cgacaccatc catgcatcgt cgggtccggc atagctcgcg
     6181 ttcgcgcgca cttcgaaatc attgcccggc tggcgggtga taacattgat cgcgccgccc
     6241 atcgtattct tgccatagag cgtgccctgc ggcccgcgca gcacctcgat gcgctcaacg
     6301 tcgagcagcg ggttgttgag ataggcggtg ttcggctgat agatgccatc gatgaacagc
     6361 ccgacgcccg gctgcaccga ctgcacgagc gtcaccccga cgccgcggat cgagacgaag
     6421 gcgcggccgg tgccgtcgct gttgatgttg agcccgggcg acagcaccgc cgcctcgcgc
     6481 accgaattga tcccgcgcgc gatcagcgtg tcgccggtga tcgcggtggc ggcgatcggc
     6541 acgtcgttca gcgtctcttc gcgcttgcgc gcggtcacga cgatcgcgcc gtcgtcttcg
     6601 ccttcggcag cggcgggcgt ggccgcgcta tcctgcgcaa agacgggcgt cgcaccaaat
     6661 gcggcggcga gcacaagcgt cgaaacggcg gcaaatcgga acttggtcat caaggcatcc
     6721 ccaaaaacgg cggtctcggc cgcccgcgga aacctatctg acagctgttg gacctatatg
     6781 aaaggttggc gattgtgccg atccgcagtc gggctttgcc gcgcgtcctc accaacagct
     6841 atatccgccg tccgccaaaa cgacgctccc cgtcatcaaa ctcgccatgt ccgacgcgag
     6901 gaagaggatt accgacgcca cctcttcggg ctcaccgagc cgcgcctgcg gcgttccgtc
     6961 gatccagcgg cgatacatgt cgccctgttt gtcggcgaag gcgttgagcg gcgtcgagat
     7021 ataggtcggc gcgaccgcat tgacccgcac acccctcccc gcccattcgg cggcgaggct
     7081 cttggtaagc tgatgcaccg cggccttcga tgcgttgtag tagctttgcg gctgcggccg
     7141 gttgacgatg aagcccgaca tcgacccgac gttgacgatg cttccctgcc ctgccgccag
     7201 catatggcgc ccgaaggcgc gcgcgcacca gaagctgccg ttgagattga cgtcgatgac
     7261 gttcagccaa tgctcgtcgg cgacatcctc ggccgcggtc tcgctgcgcg cgataacggc
     7321 attgttgacc aggatatcga tgcgaccctc gcgcgccaac atggcatcgg cggcggcgtc
     7381 gaccgccgcg ctgtgtgtga cgtcgagctc agcggcgaag atggcatatc ccttcgcgga
     7441 cagttcagcg accgcgcggt cgagcgctgc cgggtccctg tcggcaatcg tcaccttcgc
     7501 acccgcttcg gcgagcgcct ccgcagtcgc gaggccgata ccctgcgcgc cgcccgttac
     7561 gaaggcggtg cgcccgtcga ggcgcagctt gtcgagatac atggcgttac tccggtacga
     7621 aggcggccgc gggcatcgcc ggctcgtgca gcgcctcgcc gcgcgtcacc tgatagacca
     7681 tccggtaggt accgaactcg ggccagggca tcgtaccggt tcgcgcgaag cccgcccaca
     7741 agtcatgcac cgcgtcggcg agcgcctgcg gcggatcgag ccccgcaagc ccccgcggcc
     7801 cggtgacgct cgccagtgtt ttgaacacga acggcatctc gatcccatgg cacgcgccaa
     7861 gctcgccgtc gcacgcgggc gagcgccagt cgaactcgta catccatgtc ttgccctggt
     7921 gcgcttcggc atattggcgc gcgggccagc ggaagacgag gtcgtccatc gcatcggtca
     7981 tcgcctgacc cgggcgtacc cccttttgct gatagccata ggccctcagc accgccttcg
     8041 cctgcggatg cgaacggccg agcagccagc gcgcgaagag accgccgatc ttgtcacgca
     8101 cgcccgtcgg cacgaaatag aggttcatct cctcggaatt ggtgccgatg agaatctcca
     8161 cgtcgcgtcc ggcgcccttg cgcagcgcat cgagcggctt ttcgggcagc acatcgtcac
     8221 catagacggg gatgaagcgg ctgatcccgt acaccggctc gcgcccctca ccgtcgcgca
     8281 ggtcgaccgc ccaaggcttt ccgaccttgt cgagcgcatc catcgccgcg gcgtgcgaga
     8341 cggcgcggaa gccgtcggca tcggggctca cgcgcaggat cttcgccagc ttctttacca
     8401 atcgctgcgc gatgtcgatc tcgcgcacca tggcgccatg accgctctga ataatcgcgc
     8461 ggcgaaacag gcccgtggca agcggcgagg tcacgaggtc ggcaatcgcc atcgcgcccg
     8521 cgctttcgcc gaaaacggtg acatttgccg gatcgccacc gaaaaccgcg atattgcgct
     8581 gcacccatgc gagcgcaaag agcatgtcgc ggagaccaag gttggtaggg gcgcccggca
     8641 cgggcagaaa accgtcgatc cccatgcgat agttgatcgc gacgcagacg atgcccgatt
     8701 cggcaaaggc gctgccgtcg tggaccgggg cgtccttgca gccgccgacg aagccgccgc
     8761 cgtggatgaa gatcatcacg ggcgcggctt tggcatcctt cggcgcccag acattgaggc
     8821 ggagataatc gccgtcgcta ccgtcgctgc cgtgaccgac gagcggcgac gggtcgacct
     8881 tcggaaaagg cctgatccgg tgtggcgccg ccggaccggg gctctccata tcgcgcacgc
     8941 cgtcccacga ccggggcggc tccggcaatt cgaagcgcgt tggcggcgca gcatagggaa
     9001 cgccaagata ccgtataacg gagcccaagg cccgtccgcg gatcgcgccg gcttcggtct
     9061 cgatcgaggc gctatgttgc tcgtagatca tatcttcgac ttatcacctc tacccgccgc
     9121 gatcccacaa tcgtgaatgt gcttaacgga gaaagaagtg tgcgcgaccg cgaccgtgct
     9181 tcgggagcga tgcgcctttc cattttcccc agcttggcga tcattttgaa cgcttcggct
     9241 agcgtgacgc cgatgcaccg gaacgatcgt tacgaacagc cgttatcaat tttgcaactc
     9301 aaacggaggg tggtcccctc accgtcggag agagagaatg aaaaagcttc tgacaatttt
     9361 cgcccttagc ggatcgatct ttattctgtc tgcctgcaac acagttgaag gcgcaggaaa
     9421 agacgttgaa tccgccgcag actgtgctga tggtgttgaa ggcaactgct aagcacagcg
     9481 aataaattgg cttaaccggc ataggcggat cgacctcgga ccggcgcgca agcgttcgtt
     9541 atcggcatga catcggccga ggtccgccca gtcgcttttt cgcgccccgg cttcctccta
     9601 atccggatgg cgcgcctgcc agcttgcgac gcggtcgatc gcgttggcgc ggatgctcgc
     9661 atagaacagg ccatagtcgt tccggtgata gctgccgtac agccagctta gcgtatcacg
     9721 gaacggcgaa ccccaggcga catcgacgtc gagaagcatg tcggtgcacc gcgcgcccgt
     9781 cagtcccttc tcgagcggcg ccagcgttgc cgcttgccgg ggataaggcg cctccggaaa
     9841 aggcaggctg ccggggttcg acgccgcgtt cgctatgccc ttttcgttcc aggtcagcgg
     9901 attgacgcag atcgccggcg cggtgctttt gacgcgtttt ccctgccacc agaaatcgac
     9961 gtccttcgtg agcatcgacg cggcggtgcg gccttcctga ctggcgttcc acgacagcac
    10021 gcagccggtc tggtgcgcat cgtcgcatgt cggcaggccg agcgaagcga agctcgcggg
    10081 cgtataggcc cccaccgtgt aggccgcgac gagccggtcc tgcagcggcg tcccgatgat
    10141 ggcttcctgc agcagtcgaa cggcgtgccc cgttccctgg ctgtgcgaag caatgatgaa
    10201 gggccggccg cggttataat gttcgacata atagcgaaac gcgcgagcca catcgtcata
    10261 cgcaaatgct acggcatcgc cgtcgcgaag cccggcgagc gaggcttgcc gatagtgcgg
    10321 cgcgtagagc aggcagcagc cattgaacgc cgacaactgc gcgagcagta ccgggccgtt
    10381 ataggcgccc tcgacgtcgt aaggcgcgtt ccacacgtcg ttcttcagat acatggtcgg
    10441 gtggatgaaa aagacgtcgg cgggtgcgtc gcgttcgtcg atcgcggcaa agcccggcgg
    10501 taccgaacgt tcgaggccat tggcgccggg tagcgcgaac cacgccgcat tttgcgcata
    10561 gtcgggtgcc ggcggcgcct tcgtctgcgc gaacggcgcg tcaggcttgc gcgtgctttc
    10621 gaccaggctt ttcgtgatcg aacattgcga taacagcgca agcaggacga cgatcgtcgc
    10681 caggattttc cagcgcttgc ggatcttgcc ggccttgccg cgcagccagt tcggcttgcg
    10741 ctcggccggc gcgatcactt gccgccctca cgcgcggcgc ggcgcgcctc gcaggtcgcg
    10801 atgaaacccg ccgccatgaa gcgcgccata cgctccttga ccgcctcgaa atcgtccgac
    10861 aggcaaagcc cgcccgacaa tttgtcgata cgtccggttc gcccaagcgt cagcatcagc
    10921 cccgccgaca cgaaatgata gccccagaaa agatcctcct cggggcaatc gggcatcgcc
    10981 tgtttgatga tcccgatcag ccgcaacacg acatgatcga aatgagtatc catcaattcg
    11041 gcgccccatt cggcggtgtt gctcatctgc gcgccaagct ggccataatg gcgccagccg
    11101 atgccgcctt ccgaaaacag ttcgagatcg gtgtcgagga acgcccgcag cgcgccttcc
    11161 acggtcggct taccttcgac cgcccgttca taagcatcga gcgccttcat ccgccgctcg
    11221 acggtgaccg aagcccgccg cgcgatcacc gcgtcgaaca gctccttctt gtccttgaaa
    11281 taataatgga gcagcgactg gtggacgccc acctgcgccg cgacttcctt cagcgtgacg
    11341 ccatagagcc cgcgcttcga gaataattct tccgcggcgt catagatctg ctccatcgtc
    11401 gccgcgcgct ggtcggcctt ggtcggccgt ctccggttgg tctgcttcac cgccttggtc
    11461 attttatccc cttcgccgcg ggtcacaaag cccgcgggca ggcgtcgtca ttggttcagc
    11521 catgatcggc tcgcaagcgc attttgtcga tctttccggt gagcgccaac ggcatcgcgg
    11581 tacgccggac gatcgcatcc ggcagccacc agttcgcgac gcgtccccgc agcgcatcga
    11641 gcaaagtctc gtcgctgacc tcggcgccgg cgcggggttc gacgatcagc accggccgct
    11701 cgccccattt ctcgtgcttg cgcgcaacga ccgcgacgag ggctacctcg ggcaagctgc
    11761 cgacgatcgc ctcgatctcg gcaggattga tccattcgcc gcccgatttg atgaggtcct
    11821 tggcccgccc ggtgatcgac agattgccgt cggcatcgat cgtcgcgaga tcgccggtgt
    11881 cgaaccagcc gttcgggccg gtcgcctttt cgccatgacc gaaatagcgt tcgacgacgc
    11941 tgccgccctt gacccacagc cgaccctcgc cgccgcgctg atcggtcagc ggtgtgccat
    12001 gcgcgtcggt gagcaacagg tcgacgccca tcggcgggcg gcccgatttg gccgcgacgc
    12061 gggtcttcgc ggtcgccggc gtcatcgcgc cgagcggcga caattcggtc atgccccagc
    12121 tcgtctggac ggtgacgccc agtcgccgtt ccagccgctc catcagcgcc tgcggcaccg
    12181 ccgagccacc gagcaagagc cgctcgagcg agggcagttc gcctccgacc gcatcgagat
    12241 ggtcgacgag gccgagccac acggtcgcaa cgcccgccgc gacggtgacc tcctcatcga
    12301 cgatcagctt cgcaaggctc gcgccgtcct ggtcgcggcc gggaagcacg agtttcgcac
    12361 ccactgcggg cgcggcgaaa ggaaagcccc agccattggc gtgaaacatc ggcaccgcga
    12421 cgagcaccga atcctcgcag gtcagcccca tgacgtcggc ctgcagcaag tgcgtcgtgt
    12481 ggagatagtt cgaacggtgc gtgtaggtga cgcccttggg atcgcccgtc gtacccgagg
    12541 taaagcagag ccccgcaggc gcattctcgt cgaacgcgcc ccataggacg ggcgcgccat
    12601 gttcggcgag caggtcggca aggtgccaga gccgcgcacc ggtcacgacc ggcaccgcgt
    12661 cgctcgccgg gccatcgtcc atcagcacga catgctcgat cgcgggacat tgccccacca
    12721 gatcctgaac gagcggcgcg agcccgctgc tgacggcgag tacgcggttg ccggcacggt
    12781 gcatcatctt cgcaagatgg tcggcggtca gccgcgggtt gagcgtgtga cagacgatgc
    12841 cggcgcccat cgcggcgtac cagcattcca tatgcgcctg cgtattccac gcgagcgtcg
    12901 cgagatgatc gccttcgcgc agtccgagcg ccgcgaacgc ccccgacagg cggttgctgc
    12961 gctcgcagag cccggcatag tcggcccgcg ccggggcggc gccggtgacg atctcgcgct
    13021 gcccatgcca tttcgcggca tggcggagga atttgtcgag cgtcagcgca tagggttgcg
    13081 gattaccgga aatcacgggc gtgtctcttc gttatacagg gggtggcagc gacagctccc
    13141 cggacggaaa tctcaggcgg cgtcacgact tgcctttcgt ttgaggacgc gcgcgagcgc
    13201 gaggtgcagc gcgatcgcga cgaaatagaa ggggacgagg ctgtagaagg cgagctggag
    13261 cgagttgtcc gggtgcgtcg gtttcagcca gtcgctcacc gcgccgagcc aggtcggacc
    13321 caggccaagc ccgatcagat tcatgatcag cagcagcacc gcgccggcca atgtccgttg
    13381 acgcgccggc accgcgttct ggaccaacgt cactgccggg gtcaggtaga aatagttgag
    13441 gaaggtcggc ccgaggagaa agagcatcgc gagcggccat gcgggcgtgt ggacgaagcc
    13501 gatgaagaac ggcaccgcga gcagcagcga gatcgcgggg atcagcccgt acgcttccgg
    13561 ccgctcagcc gcataacgat cgacgagacg cccggagaga tagatgcccg cgctgacccc
    13621 gatgccgagg acaagcgcgt accagatcgc gatttcgtcg agcgtcatgc ccttttcccg
    13681 catcaggaac agggtcgtaa agccgagcgt cgcataggtc acgaactggg tcgcgcccgc
    13741 agcgagcgag acgagcgtca gatcggcgcg cgcgaaaaac atcttcatcg tcgcgccaaa
    13801 ggtcgcccgc tccccggcag gctcgaccgc ccgcacgaca tcggtcgcgc cgcgacgcgg
    13861 ttcgcggacg aaggcccaga cgcccgcggc ggcgatcagc cccgcgacgc cgaggagcag
    13921 gaaagcgagc cgccagtcat acgctgcggc gatctttgcg ccgaaggcga caccgagcgc
    13981 ctgcccgagc ggcggcccga gattgaacag gccaagcgcg agtccgcgcc gttcgggcgg
    14041 aaaatagtcg gagatgatcg aataggaggg cggcacgccg cccgcctcgc cgattcccac
    14101 ccccatgcgc gcggcggcaa gctgaacata ggtcgtcgac atgccgcacg cgatcgtcgc
    14161 ggcgctccat agcgcgcagg cgacggaaag cacgcgcacg cgattggtgc ggtcggcaag
    14221 ccacgcgacg gggatgccga ggacgcagta gaacatcgcg aaatagagcc cgccgagcag
    14281 cccgagttcg ccgtcggtga tgccgagatc gtcctggatc ggcttggcga ggatcgacag
    14341 cagctgccgg tcgaggaagt tgagaacgta gacgaagcac agcgcagcaa ggacgaacca
    14401 gccatagcga tcgccctgcc ggccggcagg agaaacagac ggggacgaag ctgccggcat
    14461 tgcgcgttat ttcccggaaa aaaggggcgg gcatggccgg gatttggcca tgcccgcgag
    14521 ggaggttcag aagttgcggc tgacgcgcag gccatattgg cgcggcgcgc ccgcaagccg
    14581 gcgtgcgccg ttgatcgccg cgacataatg ctggtcggtc aggttggtgc cataggccgc
    14641 gatcgaccag tcgccggtgg tcagcgtcaa ttgggcgttg aggatgttgc gcgcctccag
    14701 ccggtcgccg aacagccggt tctggaacag gctgccccaa ctcgccgcga tatgcgcgaa
    14761 gtcgacgcgc ggcgtcagcg tcgcatcgcc accgagcggg atggcatatt gggcgccggc
    14821 gctcagcgtc agcttgggcg cataggcctg ttcgttcccg gtcaggtcga gacaacgcgg
    14881 cgtcgccgga ccgttttcgg gattgcacgg agcgggtgcg ccgccgagcc gcggatcggc
    14941 ggcgaagaag gtgccgagct tggaattgga caccgatccc gcgagatcga gcgagaagcg
    15001 gtcgaagctt ccctgcgccg aaaattcgac gccatagagc ttggtcgtgc cgggcacgtt
    15061 gaagatcgac gagatgctcg gggacaccgg gtcgacgatg ctgacctgga aattcttgta
    15121 gagattgtaa tagccgccaa gctgcgtacg cagatggccg tcgaagaagg tgcctttcca
    15181 gccgagctcg aaatcggtga cgtcctccgc gtcgaacgcc agcggcgtcc ggccgaggat
    15241 attcggacca ttgaggccgc ccgccttgct gccggtggcg acgaaggcat agaggaaatg
    15301 gttcggatcg acggtccagt tcagcgcgac cttgccattg acctttttgt tgacggtccg
    15361 atcgacctgc gggagcacaa ggatcgggcc gggaatgccc gtgaaatagg cccgcgcttc
    15421 ggcgtcattc gccgacgttg tgcgcgacca gcgcccgccg atcgtgacct gcaacccgtc
    15481 ggtcaattca tagccgacct gcccgaaagc cgcgagcgcg gtcttcggat tggtgccgac
    15541 gatctcgata tcgagcgcgg tcggcgtgtt cggcgtcgca tccgaataat agccgccgtt
    15601 ttcggggatg gtgatcttgt cgtgctggta atagccgccg agcacccagg tgaaggggcc
    15661 ggtgtcgggc gagacgatgt tgaattcctg gctccagatt tcctcgctga tattgtcgcg
    15721 gaacgacagc gcggggatga tcgtggccgt gccgtcggag tcgtaggata tctgcgtcgt
    15781 gccgcgctga aagccgctga tcgagcgcag cgtcaagccg ctgtcgaact tgtatgcgac
    15841 gttgagcacg acgcggccga attcgtcgcg gccgcttaga tgggcgtcgc tcgcgacatc
    15901 gagcggatcg ccggtttcgg tagcgcggct cgcaggcagg ccgccatatt cgatattgtt
    15961 gtaatcgcct ttcagcagca ccgtgagttc gggcgtcggc tcccatgcga tgctgacgcg
    16021 cgcgctgtaa tcgcggcggt tgccgggatt gccggtccat ggacccgaca tgtcgtagaa
    16081 ggtgtcgcgc cgctccatat tgccggcaaa gcggatcgcg agcgtgtcgc tgtgcgggat
    16141 attgagcgcg ccctgcagct tcatatggtc gtaattgcca tattgggcga ggaaatagcc
    16201 gccgacgcga tcgaaggtcg ggttctgctg ggtgatgaac accgcgccgc cggtcgcgtt
    16261 gccgcccgcg aaagtccctt gcggtccgcg cagcacttcg acactggcga tgtcgtaata
    16321 gggctcgctc tggaaatagc cgggcaaggt cgcgacgccg tcgcgatagg tgatgacccc
    16381 gacgccgatc gcgctcgtct gttcggtctt gccgatgccg cggatgttga aggcgttgcc
    16441 ctgccccgtg gtgttgaccg tcagcgacgg cgaggcgaac tgtagtccct cgacgctgtt
    16501 gacccccttg cgcaccagat cctcgccggt cagcaccgtc gcggcaacgc tgctacgttg
    16561 caggctggtg gtgcggcgtt cggcggtgac gacgatctcg tcgccgaatg catcggcggt
    16621 ggcctcctcg acaggcgcag tctgagcaaa aaccggcacc ggccccagca gggcaacggc
    16681 cgaaacggaa agcagcagct tcctcatcct atcttctccc aatggcgaaa cgctctgcgg
    16741 cgttctcggg tgggagattt gcgcccaata tcctcaactg tcaacagtca ttaatatatg
    16801 aatgaataag attcattcgg aaaaaatccc cgtaaatttg ccactatttc cccatgaatc
    16861 accgagcatt tgatcaatct ttgggtcact aatttttgaa ttaaagtctc gatccatgaa
    16921 cgccccgccg cgactcgcgg tttcgctgcg tgaaatccgc aaggcgccgc cgccgtcacg
    16981 gcccggcgaa gacgctcgac cgggccatca tatccggttc gctttggcgc gcatgccgag
    17041 ataatgcgca ctcggcttca gcgtgcgctt gaaggtcttg cggtcgactt cgaccagccc
    17101 gaacttcttc gcatagcccg acgtccactc gaaattgtcg agcagcgacc agtagagata
    17161 gcccttcacc tcgatcccgt cgtcgatgca cctgctgact tcgtccatcg tcgccttgat
    17221 gaacgcgatg cgcgcggcgt cgtcggttgt gccgatcccc gtctcggtca gatagagcgg
    17281 cttgccgata tgcttggcgg cgtagcggat cgtgtgcccg accgcgggcg gataattctc
    17341 atatcccata tcggtcagcg gagcaccgga cgtcggcggc aacagcccct tcggcccgat
    17401 gcgcagccgc gaataggtct ggacgccgac gaaatcggac tggcgcgccg cctcgatcca
    17461 gccgccgtac atcgtctcct cggcctcttt gccgcgatgg ccttcaccga tgccctggat
    17521 ttcctgcatc gacagcgtca cgccgacggg caggtcggac cgcgccgcct tgatcgccgc
    17581 atagcccttg gcatgcgcat cgagcatgac cttgtcgatc ttgtcgggat cgccgaacag
    17641 catcgaggag aatttcggcg aattggtgcg ctttgccgcc gcggcgatca tcgcgcgggc
    17701 attttccgcc gcggcgtcgc tcgcgaagcg cgggatcact ttgcgcagcc caaggatgtt
    17761 cgcttcgttg aatgtcgtcg ccatgccgat caaccgcccc agcctctcgg tcgcgcggcc
    17821 acagaaacgt gcgaacaggt cggcgccatc ggcgacctcg aagccgccgc gcatcgcgaa
    17881 ccacaagggc acggtgaagt gattataggt caccatcggc atcagcccgc gcgcgcggca
    17941 agcctcgatc acgcgggcgt aatgatcgag cgcggcttcg gagaacatgc cctcggcggg
    18001 ctcgatccgc gcccattcta tgccgaagcg gtagcagttg agaccgagcg acgccgcgat
    18061 gtcgaggtct tcctcatagc gatgatagct atcgcaggcg tcgcccgacg ggtcggcaaa
    18121 ggcggtcggc ttgttcgttt cgagcaacca gctatccgaa gcggtgttgc cgccctcgct
    18181 ctggtgcgcc gagattgcgg tgccccagag aaatcctgtc ggcgcctgcc gcccggcggc
    18241 aagcgcgccg gccggcac
//
LOCUS       DATMSU010000313         6401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_52777, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000313 DATMSU010000000
VERSION     DATMSU010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52777"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VN034_08670"
     CDS             <1..198
                     /locus_tag="VN034_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWV60712.1"
                     /translation="ALGLPERTTPHALRHSFATHLLAGGADLRSLQELLGHASLASTQ
                     VYTAVDAAHLLDIYRSAHPRA"
     gene            complement(285..1793)
                     /locus_tag="VN034_08675"
     CDS             complement(285..1793)
                     /locus_tag="VN034_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HWV60713.1"
                     /translation="MKSGLSLIALALAFTAPAALHAQDKADDNKAKAEKPADYEPQVR
                     TTKLSGTFGGQRINYAATIGETIIKNKDGVPEVAVVTTSYIKEPRDPSRPITFLFNGG
                     PGSGTVWLMMGAFGPKRVAIPGNGIDDGAPPYPIVDNPDALLDVTDVVFIDPPGTGFS
                     HLIGKADPKDYYGVTQDAKLVAEVIRRWLNDNGRWNSPKFLGGESYGTTRSAAVANQL
                     MNETYNDVALNGIILISTVLDFAAGADTPGNELSPITNLPSMAATALYHGKATAPSVE
                     QFVEEARQWAIGPYATALLKGQKLQGEERAAIRRELARFTGLSETYLESADLRVTPNR
                     FYKELLRDRGLTVGRLDSRYTGKDYDSAGEGPDNDPSFYGIDASYTAAINSWSRDTLG
                     FKTDREYQSIGRIGGQWDWRLGGRDTNAYLNVAPYIGQALRENSGLRVLVAQGYYDFA
                     TPFFAAEYALSRTGIPQDRIQYTYYDAGHMMYIRDEDRHKLSADVRAFIRGR"
     gene            complement(1891..4077)
                     /gene="uvrB"
                     /locus_tag="VN034_08680"
     CDS             complement(1891..4077)
                     /gene="uvrB"
                     /locus_tag="VN034_08680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWV60714.1"
                     /translation="MAIQIRTSLDEIDTAEGYVPHRPARPDKVEGGKRFELVSDYEPA
                     GDQPTAIKELVDTALAGERDQVLLGVTGSGKTFTMAKVIDELQRPALILAPNKILAAQ
                     LYGEFKSFFPNNAVEYFVSYYDYYQPEAYVPRSDTYIEKESSVNEAIDRMRHSATRAL
                     LERDDVIIVASVSCLYGIGSVETYSAMIFDLKKGQVADSREIIRKLVALQYKRNDQAF
                     ARGNFRVRGDSLEIFPSHYEDMAWRVSFFGDEIEEITEFDPLTGKKIANLNYVRVFAN
                     SHYVTPGPTLKQASEAIRHELAERLKELEAEGRLLEAQRLEQRTNFDLEMIAATGSCA
                     GIENYSRFLTGRLPGEPPPTLFEYLPDNALLFVDESHQTIPQIGAMSKGDHRRKITLA
                     EYGFRLPSCIDNRPLRFAEWDMMRPQTVSVSATPGTWEMDRTQGVFAEQVIRPTGLID
                     PPVEIKPVEEQVDDLIAEAKKTATAGYRTLVTTLTKRMAEDLTEFLHEAGLKVRYMHS
                     DVETLERIEIIRDLRLGVFDVLVGINLLREGLDIPECGLVAILDADKEGFLRSETSLV
                     QTIGRAARNVDGRVILYADRITGSMERAMRETDRRREKQEAYNAEHGITPTTIKRNIG
                     DIIAHVASKDQVTIDIGDDKPAHMVGHNLRAYIAELEKKMRDAAADLEFEEAGRLRDE
                     IRKLEADELGLPPDEQVAPRVGRSNEGKPGTRKGRFGKQSKTKWGR"
     gene            4165..4683
                     /locus_tag="VN034_08685"
     CDS             4165..4683
                     /locus_tag="VN034_08685"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HWV60715.1"
                     /translation="MKKFLTVAAIGALVAVAGCSDKGENAGGTVKREAGNWKTDVKLV
                     KFEVPGMPPEMKDGMTKMMEGASGMDQCFTQEQVDKEDIAAELAKGPNGGGECTWSKK
                     EVAGGKINVAGTCTQNGQTVDMAMTGTMAAKKTDVTITTKGKVPTGGDMEMVMQMTST
                     HTGPCKAATTTT"
     gene            5266..>6401
                     /locus_tag="VN034_08690"
     CDS             5266..>6401
                     /locus_tag="VN034_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV60716.1"
                     /translation="MDQAKANETQDPGEIDRIIAEELGALLDSPMFTRSPVLSRLLQF
                     LVEHRLRGGRSSPKAYAIATEALGRSEDFDPAVDSYPRVMVGRLRSLLDRYYADIPWV
                     HRLRVPQGSYEVVVQYRSVPPSARSVDDPADEDDVKAAAAPSGSARPARHVRPGSRGS
                     RFGRWIVVLILLALALFTLWTLRGGSARLFVADPVPVPLLEVSPPVAGNLPQSRALAR
                     ALDGKLRDGLRRFELVDLLSSKAPGSATERTGDYRLDTSLVRTVEGDTDVTLVLNRVA
                     DQRAIWSQQMRLTSDETPEFTAIEPLIAQLAGDYGVIVRDQVQRQPENFAAGFPCLAQ
                     FNRMRQMRNAASATRVGACLRATIEANPRDPVALTALSLVRYGD"
BASE COUNT         1072 a         1947 c         2162 g         1220 t
ORIGIN      
        1 gcgctggggc tgcccgaacg gacgacgccg cacgcgctgc gccacagctt cgcgacgcac
       61 ctgctggcgg gcggcgccga tttgcgaagt ttgcaggaat tgctcgggca tgcgagcctg
      121 gcctcgacgc aggtctacac cgcggtcgat gcggcgcatt tgctcgatat ttatcgaagc
      181 gcgcatccgc gggcttgagg gccgttttgg ggtgggaagc ggagtaaagc cctctcccct
      241 tcaggggaga gagccgtccc cataacgtcc gctaccggcc gcgcttaccg cccccgtatg
      301 aacgcccgca cgtcggccga caatttatgc cggtcctcgt cgcggatata catcatgtgg
      361 cccgcatcat aataggtgta ttggatgcgg tcctgcggga tgccggtgcg cgacagcgca
      421 tattcggcgg cgaaaaaggg ggtcgcgaaa tcataatagc cctgcgcgac gagcacacgc
      481 agtcccgaat tctcgcgcag cgcctgcccg atatagggcg cgacgttcag ataggcgttg
      541 gtgtcgcggc cgccgaggcg ccagtcccac tggccgccga tccggccgat cgactgatat
      601 tcgcggtcgg tcttgaagcc gagcgtgtcg cggctccagc tgttgatcgc ggcggtatag
      661 ctcgcgtcga tgccatagaa gctggggtcg ttgtcggggc cttcgccggc gctgtcataa
      721 tctttgcccg tataacggct gtcgaggcgg ccgacggtca ggccgcggtc gcgcagcagt
      781 tccttgtaga agcggttcgg cgtgacgcgc aggtcggcgc tttcgagata ggtttcggag
      841 aggccggtga agcgcgccag ctcgcggcgg atcgcggcgc gttcctcgcc ctgcaacttc
      901 tgccccttga gcagcgcggt ggcatagggg ccgattgccc attgccgcgc ttcctcgacg
      961 aattgctcga ccgacggcgc ggtcgccttg ccatgataga gcgcggtcgc ggccatcgag
     1021 ggcagattgg tgatcggcga cagctcgttg cccggcgtgt cggcgcccgc cgcgaaatcg
     1081 agcacggtcg agatcagaat gatgccattg agcgcgacgt cattgtacgt ctcgttcatc
     1141 aactggttgg cgaccgcggc ggaacgcgtc gtgccatagc tttcgccgcc gaggaacttg
     1201 gggctgttcc agcggccatt gtcgttcagc cagcggcgga tcacctcagc gacgagcttc
     1261 gcgtcctgcg tgacgccgta ataatccttg gggtcggcct tgccgatcag gtgcgaaaaa
     1321 cccgtgcccg gcgggtcgat aaacacgacg tcggtgacgt cgagcagcgc gtcgggattg
     1381 tcgacgatcg gatagggcgg cgcgccgtcg tcgatgccgt tgccggggat cgcgacgcgc
     1441 ttcggcccga aggcgcccat catcagccag acggtgccgg acccggggcc gccgttaaac
     1501 agaaaggtga tcggccggct ggggtcgcgc ggttccttga tgtatgaggt ggtgacgacc
     1561 gcgacttcgg gcacgccatc cttgttcttg atgatcgtct cgccgatcgt cgcggcataa
     1621 ttgatgcgct ggccgccgaa ggtgcccgaa agcttggtcg tgcgaacctg cggctcatag
     1681 tccgcgggct tttcggcctt cgccttgttg tcatcggcct tgtcctgcgc gtggagcgcg
     1741 gcaggcgcgg tgaaggcaag cgccaaggcg atgagcgaca gacccgattt catatatgta
     1801 tctccccgat tgcggggcga ccctatgcag cgcgcgccgg ggcggcaagg ttacttatcg
     1861 gttacaggcg ggggttcgtc gaagggggag tcaacgcccc cacttcgtct tgctctgttt
     1921 cccgaaccgc cccttgcgcg tccccggctt gccctcgttc gagcggccga cgcgcggggc
     1981 gacctgttcg tcggggggca ggccgagttc gtcggcttcg agcttgcgga tttcgtcgcg
     2041 caggcggccg gcttcctcga actcgaggtc ggcggcggcg tcgcgcatct tcttttcgag
     2101 ctcggcgata taggcgcgca gattatgccc gaccatgtgc gccggcttgt cgtcgccgat
     2161 gtcgatcgtg acctgatcct tcgacgcgac atgcgcgatg atgtcgccaa tgttgcgctt
     2221 gatcgtcgtc ggggtgatgc catgctcggc gttataggct tcctgctttt cgcggcggcg
     2281 gtcggtttcg cgcatcgcgc gttccatgct gccggtgatg cgatcggcgt agaggatgac
     2341 gcggccgtcg acgttgcgcg cggcgcggcc gatcgtctgc acgaggctcg tctcgctacg
     2401 cagaaaacct tccttgtcgg catcaaggat cgcgacgagc ccgcattcgg gaatgtcgag
     2461 cccttcgcgc agcaggttga tcccgacgag cacgtcgaac acgccgagcc tgaggtcgcg
     2521 gatgatctcg atgcgctcga gcgtttcgac gtcgctgtgc atatagcgga ccttgagtcc
     2581 ggcttcgtgg aggaattcgg tgagatcctc ggccatgcgc ttggtgagcg tcgtcaccag
     2641 cgtgcgatag cccgctgtgg cggtcttctt cgcctcggcg atcaggtcgt cgacctgttc
     2701 ctcgaccggc ttgatctcga ccggcgggtc gatgagtccc gtgggacgga tcacctgttc
     2761 ggcgaagacg ccctgcgtgc ggtccatctc ccacgtcccc ggggtcgccg atacgctgac
     2821 cgtctgcggg cgcatcatgt cccattcggc gaagcgtaag ggccgattgt cgatgcagct
     2881 cggcaggcgg aagccatatt cggcgagggt gatcttgcgg cgatggtcac ccttcgacat
     2941 cgcgccgatt tgcgggatcg tctggtggct ctcgtcgacg aagagcaggg cgttgtcggg
     3001 cagatattcg aacagcgtcg gcggcggctc gccggggagg cggccggtga ggaagcggct
     3061 gtaattctcg atccccgcgc agcttcccgt tgcggcgatc atctcgaggt cgaaattggt
     3121 gcgctgttcg agccgctggg cttcgagcag gcggccttcg gcctctagct ccttgaggcg
     3181 ctcggcgagt tcgtgccgga tcgcttcgct cgcctgcttg agcgtcggac ccggggtcac
     3241 atagtgcgag ttggcgaaga cgcggacata gttgaggttg gcgatcttct tgcccgtcag
     3301 cgggtcgaac tcggtgatct cctcgatctc gtcgccgaag aagctgacgc gccacgccat
     3361 atcctcatag tgcgagggga agatttcgag agagtctccc cgcacgcgga aattgccgcg
     3421 cgcgaacgcc tgatcgttgc gcttgtattg cagcgcgacg agtttgcgga tgatctcgcg
     3481 gctgtcggcg acctggccct tcttcaaatc gaagatcatc gcggagtaag tttcgaccga
     3541 gccgatgccg tagaggcacg agaccgaggc gacgatgatc acatcgtcgc gttcgagcaa
     3601 agcgcgcgtc gccgagtggc gcatgcggtc gatcgcctcg tttaccgagc tttccttctc
     3661 gatataggtg tccgaccgcg gcacataggc ctcgggctgg taatagtcgt aatagctgac
     3721 gaaatattcg accgcattgt tcgggaagaa gctcttgaac tcgccgtaga gctgtgccgc
     3781 gaggatcttg ttcggcgcga ggatcagcgc ggggcgctgc aattcgtcga tgaccttcgc
     3841 catggtgaag gtcttgcccg atccggtgac gccgagcagc acctggtcgc gctcgcccgc
     3901 cagcgcggtg tcgaccagtt ccttgatcgc ggtcggttgg tcgcccgcgg gttcatagtc
     3961 gctaaccagt tcgaatcgct tgccgccttc gaccttgtcg ggacgggcgg ggcgatgggg
     4021 gacatagcct tcggcggtgt cgatttcgtc gagcgaggtg cgaatctgga ttgccatcgc
     4081 cgccaatatg gtatcgatgg tgcgggtcca caatcgcaat gaacagaaca acgcccccta
     4141 atggatagtg acaggagaca gggtatgaag aaatttctga cggtcgccgc catcggcgcg
     4201 ctggtcgcgg tggcgggttg cagcgacaag ggcgaaaatg ccggcggcac ggtgaagcgc
     4261 gaggccggca attggaaaac cgacgtcaag ctggtgaagt tcgaagtccc cggcatgccc
     4321 cccgaaatga aggacggcat gacgaagatg atggaaggcg cgagcggcat ggaccagtgc
     4381 ttcacgcagg agcaggtcga taaggaagac atcgcggccg aactcgccaa ggggccgaac
     4441 ggcggcggcg aatgcacctg gtcgaagaaa gaggtcgcgg gcggcaagat caacgtcgcg
     4501 ggcacctgca cgcagaacgg ccagacggtc gacatggcga tgacgggcac gatggcggcc
     4561 aagaagaccg acgtcacgat cacgaccaag ggcaaggttc cgaccggcgg cgacatggaa
     4621 atggtcatgc agatgaccag cacccacacc ggcccgtgca aggccgcgac gacgacgacc
     4681 tgagcgcccg cgctttaatc gaacgaacaa caaaggggcg tcagtgcaag ctggcgcccc
     4741 ttgatcattg aggccgccct gcaagacgat cgaatgcccg gctgggccct ccacatccga
     4801 gccgtccgaa ggagcccggc gacgcggggc cgcgctcgtg gcgtcggaag gggaggtgct
     4861 gaggagaggc agtcgacttg ccgggcgaca gaacaggtgg ggctgtcggg ccgcccggcg
     4921 aagtcttgaa atgaagcctg agggggatcg aaaggtgcca gggaccagct cgcaccattc
     4981 gccttttggc ttcacaccgc gtcatcgtag gaatgccgcg acgtcggaaa ggatgccaga
     5041 gtttcgtgtt tgcggcagtt acaaggggtc acaaagcgca atgttgccat ttgataacca
     5101 attcttaacc tgatttcgcg tttttccttg cggattggcg cggcgatttt cttctgatat
     5161 ggcacaaggt tgccgtgtta ccgttacgtc aaagtaacaa agggtggccg cgctggctga
     5221 ttggcgtatc taccgcaccg cccgtcaggg gtattcgagg gtagcatgga ccaggcgaaa
     5281 gcgaacgaga cacaggatcc gggcgagatc gaccggatca tcgccgaaga actcggggcg
     5341 ctgctcgatt ccccgatgtt cacgcgttcg ccggtgctgt cgcggctgtt gcaatttctt
     5401 gtcgagcatc gcctgcgcgg cggtcgaagc tcgcccaagg cctatgcgat cgcgaccgag
     5461 gcgctggggc gcagcgaaga tttcgatccc gccgtcgaca gctatccgcg cgtcatggtc
     5521 ggccggctgc gcagcctgct cgaccgctat tatgccgaca tcccgtgggt tcaccgcctg
     5581 cgcgtgccgc aaggcagcta tgaggtggtt gtccaatatc gctcggtgcc gccgtcggcg
     5641 cgatcggtcg acgacccggc ggatgaagat gacgtcaagg ccgcagccgc cccgtcgggt
     5701 tccgctcgcc ctgccaggca cgtgcggccc ggcagtcgcg gttcgcggtt cggccggtgg
     5761 atcgtcgtgc tcatccttct cgcgctcgcg ctctttacct tgtggacact tcgcggaggc
     5821 tccgcgcggc tcttcgtcgc cgatcccgtc cccgttcctt tgctcgaagt cagcccgccg
     5881 gtagcgggca acctgccgca gtcgcgggcg ttggcgcgcg cgctcgacgg caagctgcgc
     5941 gacggactcc ggcgcttcga actggtcgat ctgctgagtt cgaaggcgcc gggcagcgcc
     6001 accgagcgaa cgggggacta ccggctcgat acctcgctgg tgcgcacggt cgaaggcgac
     6061 accgatgtca ccctcgtcct caatcgcgtc gccgaccagc gcgcgatctg gtcgcagcag
     6121 atgcgcctga ccagcgacga aacccccgaa ttcacggcga tcgaaccgct gatcgctcag
     6181 ctcgccggcg attatggtgt gatcgtgcgc gaccaggtcc agcgacagcc cgagaatttc
     6241 gcggcgggct ttccctgcct cgcgcagttc aaccggatgc gccagatgcg caatgcggcg
     6301 tcggcgacgc gggtcggcgc ctgcctgcgc gcgacgatcg aggcgaatcc gcgcgatccg
     6361 gtcgcgctga ccgcgctgtc gctcgtgcgc tatggcgact g
//
LOCUS       DATMSU010000314         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_52786, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000314 DATMSU010000000
VERSION     DATMSU010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52786"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VN034_08695"
     CDS             <1..915
                     /locus_tag="VN034_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV60717.1"
                     /translation="QVEEDAVDVLIGFGRAPVGLGEAGVAADMMALRRQLILFFDEGI
                     DAIAFGRVVQQRFARAILELGGNNGVIVAPSADLDLALRGVAFGAMGTAGQRCTTTRR
                     LFLHDSIYDGFVAKLKAAYASVGVGNPLDGEVLVGPLIDRAAYEQMQDALKAAAAADG
                     VVHGGERVGEGASFYVKPALVEMPGQVGPVLEETFAPILYVMRYYDLDAVIEQHNDVA
                     AGLSSAIFTTDMREAERFLVASDCGIANVNLGTSGAEIGGAFGGEKETGGGRESGSDS
                     WKAYMRRATNTVNYSDALPLAQGVSFEI"
     gene            complement(1031..>2133)
                     /locus_tag="VN034_08700"
     CDS             complement(1031..>2133)
                     /locus_tag="VN034_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWV60718.1"
                     /translation="SGEDKSYNAIRKVATRREPNYAQKHGLAAPVDVYPLYENATRAA
                     WGESLEDAQFESAEIWSRFSEVAAANDGAWIRKPASPADILRVDERNRPIAFPYSKLM
                     VANSSVNQGAGFIVASLAEAWRRGIPEDRLIHIGMGAAAKEPPSILHRDRYDGSVSME
                     TSIMRTLELNGMTAADFDFVELYSCFPCVPKMARRILGWPWDRPASVFGGLTFGGGPI
                     ANYMSHAIVSMVDKLRAEGRYGFLFANGGFATDNHCIVLGSQPIAAASFPQDFDYQAE
                     AEAKRGPVPELVEDYAGPATIESYTVFYGRDGAPKAGVVVALTPEGKRTLAHVDVGDG
                     AMLAFLTDGKQEPIGTTGQVVALEEGFGWHMA"
BASE COUNT          357 a          656 c          734 g          386 t
ORIGIN      
        1 caggttgagg aggacgcggt cgacgtcctg atagggtttg gccgcgcgcc agtcggactg
       61 ggtgaagccg gggtggccgc cgacatgatg gccctgcgcc gccagctcat tctgtttttc
      121 gacgaaggca tagatgcgat cgcttttggc cgggtcgttc agcagcgttt cgcgcgcgcg
      181 atcctcgaac tcggcggcaa taatggtgtg atcgtcgcgc cgtcggccga tctcgatctc
      241 gcgctgcgcg gggtcgcgtt cggggcgatg gggacggcgg gacagcgctg cacgacgacg
      301 cggcggttgt tcctgcacga cagcatttat gacggcttcg tcgccaagct gaaggcggcc
      361 tatgcgagcg tcggcgtggg caatccgctg gacggcgagg tgctcgtcgg gccgctgatc
      421 gaccgcgctg catatgagca gatgcaggac gcgctgaagg cggcggccgc ggcggacggg
      481 gtcgtccatg gcggtgagcg ggtcggcgag ggcgcgagct tctatgtaaa gcccgcgctc
      541 gtcgagatgc cgggacaggt cgggccggtg ctcgaagaga cgttcgcgcc catcctttac
      601 gtcatgcgct attacgacct cgatgcggtg atcgagcagc acaatgatgt cgccgcagga
      661 ctgtcgtcgg caatcttcac gaccgatatg cgcgaggccg agcggtttct cgtcgcgagc
      721 gactgcggga tcgccaacgt caacctcggc acgtcgggcg ccgagatcgg cggcgcgttc
      781 ggcggcgaga aggagacggg cggcgggcgc gagagcggct ccgacagctg gaaggcctat
      841 atgcgccgcg cgacgaatac ggtgaattat tcggatgcgc tgccgctcgc gcagggggtt
      901 tcgttcgaga tttgacagcg agaggccacc ttcaattccg ttcgtgtcga gcgaagtcga
      961 gacaccgtga aggcatgttc gaccgatggg catctcgact tcgctcgatg cgaacggaaa
     1021 agggacggcg tcacgccatg tgccagccga agccttcttc gagggcgacg acctgacccg
     1081 tagtcccaat cggctcctgc ttcccgtcgg tcagaaatgc cagcatcgcg ccgtcgccaa
     1141 catcgacatg cgcgagcgtg cgcttgcctt cgggtgtcag cgcgacgacc acgcccgctt
     1201 tcggcgcgcc atcgcggcca taaaacaccg tatagctctc gatcgtcgcc ggcccggcgt
     1261 aatcctcgac cagttcgggg accgggccgc gtttcgcttc ggcctcggcc tgatagtcga
     1321 aatcctgcgg gaaactcgcc gcagcgatcg gctgactccc cagcacgatg caatgattgt
     1381 cggtcgcgaa gccgccgttg gcgaagagga agccatagcg gccttcagcg cgcaatttgt
     1441 cgaccatcga gacgatcgca tggctcatat agttcgcgat cggcccgccg ccgaaggtca
     1501 gcccgccgaa cacgctcgcg gggcggtccc acggccagcc gaggatgcgc cgcgccatct
     1561 tgggcacgca cgggaagcag ctgtaaagct cgacgaaatc gaaatccgca gcggtcatac
     1621 cgttcagttc gagcgtgcgc atgatcgagg tttccatgct gacgctgccg tcgtaacggt
     1681 cgcggtggag gatgcttggc ggctcctttg ccgcggcgcc catgccgata tggatcaggc
     1741 gatcttcggg gatgccgcgg cgccatgctt cggcgaggct cgcgacgatg aaccccgcgc
     1801 cctgattcac cgacgaattg gcgaccatca gcttcgaata agggaaggcg atcgggcggt
     1861 tgcgctcgtc gacgcggagg atgtcggcgg gcgaggcggg tttgcggatc cacgcgccgt
     1921 cgttcgcggc ggcgacctcc gaaaagcgcg accagatttc ggcgctttcg aattgggcgt
     1981 cctcaaggct ttcgccccac gccgcgcgcg tcgcattctc gtagagcgga tagacgtcga
     2041 ccggcgcggc gaggccgtgc ttctgcgcat aattgggttc gcgccgcgtc gcgaccttgc
     2101 ggatcgcatt atagcttttg tcctcgccgc tcg
//
LOCUS       DATMSU010000315         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_138239,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000315 DATMSU010000000
VERSION     DATMSU010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138239"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VN034_08705"
     CDS             complement(<1..320)
                     /locus_tag="VN034_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HWV60719.1"
                     /translation="MADGTPRNPIVAEQAADRLYIIDARHGVERRILLDWIHSTSGDD
                     EPAWASLDIEDGDHALPVDVLQARLGGAASRQVVPLRVAWRIPGFDKGRALKFRHLIF
                     GDPR"
     gene            complement(324..1904)
                     /locus_tag="VN034_08710"
     CDS             complement(324..1904)
                     /locus_tag="VN034_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HWV60720.1"
                     /translation="MPRPQPHLEEVLGSEPGPHIAALFDFDGTIISGYSATAMLREKF
                     QRREMSVEEIAETANVLAQHSLGSIGFSGLMTAAAKFMKGVDEQSFVEFGEELYRKHI
                     ARKIYPETRAIIEAHQAKGHRVAIISSATVYQIEPTARDLGITDIKCSAYEIEDGVFT
                     GEIIRPLCFGEGKVLAAEELAAEYGLDLDNSFFYSDSDDDIELLERVGKPRPLNPNLK
                     LKGIADERNWPVQRFASRGTPSWVDYTRTIYATGSLVGAFAAGLPIWALTRSQREAVN
                     FSMGLFGDFATAITGVELEVEDERHLWSSRPCVFIFNHQSKADVMILAKLIRRDMGGV
                     GKKEIKDIPILGKLMEWGGTVFVDRADGKSAIKAMEPLVDAIQKEGKSICIAPEGTRS
                     LTPKLEPFKKGAFHLAMQAGVPIVPIVIHNATDVAPKNEFVMRPATVRVTVLPPVDTS
                     GWSVRTINSHVRDVRNMFLRTLGQVEESAAESVATEAATPKEKVEKAKPKKAAEKKPV
                     AKKKAPAKKPAARKGKTG"
     gene            complement(2113..2733)
                     /gene="eda"
                     /locus_tag="VN034_08715"
     CDS             complement(2113..2733)
                     /gene="eda"
                     /locus_tag="VN034_08715"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HWV60721.1"
                     /translation="MADSGIEQIMRLAPVIPVIVIDRVEDAVPMAEALVAGGLKVLEV
                     TMRTPAALDAIAAMKKVPGAVVGAGTVLNPRMLNAALDAGSEFIVSPGLTDTLGEAAV
                     ASSIPFLPGVANASNIMTGLDLGLDSFKFFPAATSGGIPALKALAGPFGGINFCPTGG
                     ISAATAPEWLALGPVLCVGGSWVVPSGPLDAVKIEALAREAAALSR"
BASE COUNT          495 a          972 c          906 g          566 t
ORIGIN      
        1 tggcgcgggt cgccgaagat caggtgacgg aatttgagcg cgcggccctt gtcgaaacct
       61 ggaatgcgcc atgcgacgcg cagcggcacg acctgccgcg aggccgcgcc gccaagccgc
      121 gcctgcagca cgtcgacggg cagcgcatga tcgccatcct cgatgtcgag gcttgcccag
      181 gcaggttcgt cgtcgccgct ggttgaatga atccagtcga gcaaaatgcg ccgctcaacg
      241 ccgtgccgcg cgtcgatgat ataaagccgg tcggccgcct gctccgccac gatcgggttg
      301 cggggcgttc cgtcggccac cgcttagccg gtctttccct tgcgcgcggc cggtttcttc
      361 gccggtgcct ttttcttggc cacgggcttt ttttcagccg cctttttcgg cttcgctttc
      421 tcgacctttt ccttcggcgt cgcggcctcg gtcgcgaccg attccgccgc gctctcctcc
      481 acctgcccca gcgtgcgcag gaacatattc cgtacgtcgc ggacgtgcga attgatcgtc
      541 ctgacgctcc atcccgaggt gtcgaccggc ggcaacacgg tgacgcgcac cgtcgcgggg
      601 cgcatcacga attcgttctt cggcgcgacg tcggtcgcgt tatggatcac gatcggcacg
      661 atcggcacgc ccgcctgcat cgccaagtgg aacgcgccct tcttgaaagg ttcgagcttc
      721 ggcgtcaggc tgcgcgtccc ttcgggcgcg atgcagatcg acttgccttc cttttgaatc
      781 gcatcgacca gcggctccat cgccttgatc gcgctcttgc cgtcggcgcg gtcgacgaag
      841 acggtgccgc cccactccat cagcttgccg aggatgggga tatccttgat ctccttcttt
      901 cccaccccgc ccatatcgcg acggatgagc ttggcgagga tcatcacgtc cgccttgctc
      961 tggtggttga agatgaagac gcaggggcgc gacgaccaga ggtggcgctc atcctcgact
     1021 tcgagctcga cgccggtgat cgcggtcgcg aagtcgccga acagccccat cgagaaattg
     1081 accgcctcgc gctgcgaacg cgtcagcgcc cagatcggca gccccgcggc aaaggcgccg
     1141 acgagcgagc cggtggcgta gatggtgcgc gtataatcga cccacgacgg cgtgccgcgg
     1201 ctcgcgaaac gctgcaccgg ccaattgcgc tcatcggcga tgcccttcaa tttgaggttc
     1261 gggttgagcg ggcgcggctt gccgacgcgc tcgagcagtt cgatatcgtc gtcgctatcc
     1321 gagtagaaaa agctgttgtc caaatcgagg ccatattcgg cggcgagttc ctcggcggcg
     1381 agtaccttgc cctcgccgaa acagagcggg cggatgatct cgccggtgaa gacgccatcc
     1441 tcgatctcat aggccgagca tttgatgtcg gtgatgccga ggtcgcgcgc ggtcggttcg
     1501 atctggtaga ccgtcgccga cgagatgatc gcgacgcggt ggcccttcgc ctgatgcgcc
     1561 tcgatgatcg cgcgcgtctc gggatagatt ttccgcgcga tatgcttgcg gtagagttcc
     1621 tcgccgaatt cgacgaagct ttgctcgtcg acgcccttca tgaatttggc ggcggcggtc
     1681 atcaggcctg aaaagccgat gctgccaagg ctgtgctgcg cgaggacgtt cgcggtctcg
     1741 gcaatctcct cgaccgacat ttcgcgccgc tggaacttct cacgcagcat cgcggtcgcc
     1801 gaatagcccg agatgatcgt cccgtcgaaa tcgaagagtg cggcgatatg cggtccgggt
     1861 tcggagccaa gaacttcttc caaatgcggt tgcggtctcg gcacgcaatt ctccccacca
     1921 acggcttatt gccgccttat ccctgcacga tgacaggtaa gatggggtta atcaatggcg
     1981 ggaaagtgcg gatgactgtc cgcattttcg tcaccccggc gaaggccggg gtctcaccct
     2041 atcgctatgg cgcaccggcg agatcgtgtt tcagagtcac gtgcctctga acacccttcg
     2101 ccgggatgac gattaacggg ataacgccgc cgcttccctt gccagcgcct cgatcttcac
     2161 tgcatcgagc ggccccgacg gaacgaccca gctgccaccg acgcagagca ccggacccag
     2221 cgcgagccac tcgggtgcgg tcgcggcgct gatcccgccg gtcggacaga aattgatccc
     2281 gccgaacggg cccgcgagtg ccttcagcgc cgggatgccg ccgctcgtcg ccgcggggaa
     2341 gaatttgaag ctgtcgaggc cgaggtcgag ccccgtcatg atgttcgacg cattggcgac
     2401 gccaggcagg aatggaatgc tactcgccac cgccgcctcg cccagagtgt cggtaaggcc
     2461 cggcgagacg atgaattcgg aacccgcgtc gagcgcggcg ttcagcatcc gcggattgag
     2521 caccgtcccc gcaccgacga cggcgcccgg gaccttcttc atcgcggcga tcgcatcgag
     2581 cgcggcgggg gtgcgcatcg tcacttcgag caccttgagc ccccccgcga caagcgcctc
     2641 ggccatcggc accgcatcct cgacgcggtc gatgacgatc accgggatga ccggcgccaa
     2701 gcgcatgatc tgttcgatac ctgaatctgc catttcaatc tcttctttca tcgtcatccc
     2761 agcgaaagct gggatcggtt tgatccttgc gcctggcccg ccagcgatcc cagctttcgc
     2821 tgggatgacg gaaaaattag accacggttt ccatcgccgc gagcatcgcc gatccgccct
     2881 tctcggcttc atcggcgtga tggcggaaca gcgcgaagag ttcgcggccg acgccgacc
//
LOCUS       DATMSU010000316         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_81096, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000316 DATMSU010000000
VERSION     DATMSU010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81096"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VN034_08720"
     CDS             <1..625
                     /locus_tag="VN034_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60722.1"
                     /translation="STAHLFALPGCAWLGLRARAWARTLSSMVPRILASAMAVLTLPL
                     LGSMAVAAVLTPLVPALKDEKQRAATKKAEANPYKASCLDPAAIADLNHLRATTLLTP
                     IDLGAPLIFWTRHSLVATPHHRNRHAMADTIRAFAGDPAKAEALVRRQRAALVVFCRT
                     ANDFSNYRRARKDSLAAQLYAGTPPAWLESVPIGSSAGLSVWRVKPE"
     gene            complement(629..1114)
                     /gene="msrB"
                     /locus_tag="VN034_08725"
     CDS             complement(629..1114)
                     /gene="msrB"
                     /locus_tag="VN034_08725"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWV60723.1"
                     /translation="MIERRYLLGGLALGGAIIGAPMLFAKSARPKAQAGWQLSDAEWK
                     KRLSPLQYRVLREAATERAFTSPLDKEKRAGTFVCAGCALPLYSSKTKFDSGTGWPSF
                     WAPLKGAIGTSTDRDLGYARTEVHCRRCGGHLGHVFNDGPKPTGKRYCMNGAALGFRA
                     A"
     gene            complement(1158..2393)
                     /locus_tag="VN034_08730"
     CDS             complement(1158..2393)
                     /locus_tag="VN034_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV60724.1"
                     /translation="MATAAAPQPVTDRYDMSPAAIYSEDRWQAPFAEIRKAGGIVKCE
                     DSVHGPYWNIASHQAIQHVEALPDIYSSSWEYGGITILERDEDEDFKLPMFIAMDRPQ
                     HTDQRRTVAPAFTPAEMTRLSEDIRNRTGEVLDGLPWGETFDWVDRVSMELTTQMLAL
                     LFDFPWEDRRKLTEWSDWMGDVELAQKVELKEQRRAVLEEAFVYFQKLWMEKLDQPPT
                     PDLLSMMLHSEAMSHMDKYEFIGNLVLLIVGGNDTTRNTMSGLAYGLDKYPDQRAKLE
                     ADPSLIPNAVSEIIRWQTPLAHMRRTALADADLFGHQIKKGDKLILWYISANRDESVF
                     DDGDALRLDRENARRHLSFGYGVHRCVGARLAELQLRILLEEMAARRMRVIVAGEPER
                     VHACFVHGFRALPVQIEKY"
     gene            complement(2643..>2855)
                     /locus_tag="VN034_08735"
     CDS             complement(2643..>2855)
                     /locus_tag="VN034_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV60725.1"
                     /translation="ARFAQIFPFSSPLAMAARAATDDAKAIHLLALGWQALWVALVIW
                     LSVRLFRAGVLSGGSGWKFWRGKKAA"
BASE COUNT          457 a          908 c          983 g          507 t
ORIGIN      
        1 gtcgaccgcg catctctttg ccttgccggg ctgcgcctgg ctcggcctgc gcgcccgggc
       61 gtgggcgcgg acactttcgt cgatggtgcc gcgcatcctc gcgtcggcga tggcggtgct
      121 gacgctgccg ctgctcggca gcatggcggt cgccgcagtg ctgacgccgc tggttcccgc
      181 gctcaaggat gagaagcaac gcgcggcgac gaagaaggcg gaagccaacc cctataaagc
      241 gagctgcctc gaccccgcgg cgatcgccga tctcaatcat ctgcgagcga cgacgctgct
      301 gacgccgatc gacctcgggg cgccgctgat attctggacg cggcacagcc ttgtcgcgac
      361 gccgcatcat cgcaacaggc acgcgatggc cgacacgatc cgcgcctttg cgggcgatcc
      421 cgccaaggcc gaggcgctgg tgcggcggca gcgggcggcg ctggtcgtct tttgccgcac
      481 cgcgaacgac ttcagcaatt atcgccgcgc acggaaggat tcgctggccg cgcagctcta
      541 tgcggggacg ccgcccgcct ggctcgaatc ggtgccgatc ggatcaagcg cggggctgtc
      601 ggtgtggcgg gtgaagcccg agtaaagatc aggcggcgcg gaagccgagc gcggcgccgt
      661 tcatgcaata gcgtttgccg gtcggctttg gcccgtcgtt gaagacatgg cctaggtggc
      721 cgccgcagcg ccggcaatgc acttcggtgc gcgcatagcc gaggtcgcgg tcggtcgagg
      781 tgccgatcgc gcctttgagc ggcgcccaga agctcggcca gccggtgccg ctgtcgaact
      841 tggtcttgct cgaatagagc ggcaatgcgc agccggcgca gacgaaggtg ccggcgcgtt
      901 tttccttgtc gagcgggctg gtgaaggcgc gctcggtcgc cgcttcgcgc agcacgcgat
      961 attggagggg cgacaggcgc tttttccatt cggcgtcgct gagctgccag ccggcctgcg
     1021 ccttgggccg cgccgatttg gcgaaaagca tcggcgcgcc gatgatggcg ccgccgagcg
     1081 cgagtccgcc gagaagataa cgacgttcga tcatgtcttc gatacgtatg acgcggccgc
     1141 agggttacaa gcgggcgtca atatttctcg atttgcaccg gcagcgcgcg gaagccgtgg
     1201 acgaagcagg cgtggacgcg ttcgggctcg cccgcgacga tcacgcgcat ccgccgcgcc
     1261 gccatttcct cgagcaggat gcgcagctgc agctcggcga ggcgcgcgcc gacgcagcgg
     1321 tggacgccgt aaccgaagga caggtggcgg cgggcattct cgcggtcgag ccgcagcgcg
     1381 tcgccgtcgt cgaacacgct ctcgtcgcgg ttcgccgaaa tataccagag gatgagcttg
     1441 tcgccctttt tgatctgatg cccgaagaga tccgcatcgg cgagcgcggt gcggcgcatg
     1501 tgcgcgagcg gggtctgcca gcggatgatc tcgctcaccg cattggggat gagcgacgga
     1561 tcggcttcga gcttcgcgcg ctggtcggga tatttgtcga gcccataggc gagccccgac
     1621 atggtgttgc gtgtcgtgtc gttgccgccg acgatcagca gcacgaggtt gccgatgaac
     1681 tcatatttgt ccatgtggct catcgcctcg ctatggagca tcatcgacaa gaggtcgggg
     1741 gtcggcggct ggtcgagctt ttccatccac agtttctgga aatagacgaa cgcctcttcg
     1801 agcaccgcgc ggcgctgttc cttgagttcg accttttgcg cgagttcgac gtcgcccatc
     1861 cagtcggacc attcggtcaa tttgcgccgg tcttcccacg gaaagtcgaa cagcagcgcg
     1921 agcatctgcg tcgtcaactc catcgacacg cggtcgaccc agtcgaacgt ctcgccccac
     1981 ggcaggccgt cgagcacctc gccggtgcga ttcctaatgt cttcgctgag gcgcgtcatt
     2041 tcggcggggg tgaaggcggg ggcgacggtg cggcgctggt cggtgtgctg cggccggtcc
     2101 atcgcgatga acatcggcag cttgaaatcc tcgtcctcgt cgcgttcgag gatcgtgatg
     2161 ccgccatatt cccacgagga gctgtagatg tcgggcagcg cctcgacatg ctggatcgcc
     2221 tgatggctcg cgatattcca ataggggccg tggacgctgt cctcgcattt gacgatgccg
     2281 cccgccttgc ggatctcggc gaagggcgcc tgccagcgat cctccgaata gatcgcggcg
     2341 gggctcatgt catagcgatc ggtgacgggc tggggcgcgg ctgcagtcgc catcgggcat
     2401 cctcctcctc aaaagggact cgccgcgacg cttgccgccg tcggccagat cagttgcagg
     2461 atgccactgt ttacagcttt gtcaacgagg ctgggacgaa caagctcctc cctgtcgcga
     2521 agcgatgggg aggtggcagc ccgtagggct gacggagggg ctaatggcgc gaggtcgcgg
     2581 cccctccacc actcgcttcg cgagcgatcc ccctccccac ggcttcgccg cagggaggat
     2641 tttcaggcag cttttttgcc gcgccagaat ttccagccgc tgccgccgct gagcacgccg
     2701 gcgcggaaca ggcgcaccga aagccatatg acgagcgcga cccagagcgc ctgccagccg
     2761 agcgcgagca ggtggatcgc cttcgcatcg tcggtcgcgg cgcgtgccgc catcgcgagc
     2821 ggcgaggaga agggaaagat ttgcgcgaag cgggc
//
LOCUS       DATMSU010000317         1762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_90933, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000317 DATMSU010000000
VERSION     DATMSU010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90933"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VN034_08740"
     CDS             <1..1209
                     /locus_tag="VN034_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV60726.1"
                     /translation="GAEIANGTVLMEDGKIAAVGAALAVPGGYRTVDARGKWVTPGVI
                     DAHSHLGAWAQPESVEMHNDVNEMTDPNTAQVWIEHSVWPQDPGFDAAREAGVTTMMV
                     LPGSGNLFGGRTVTLKNVPSVTMQGMKFPGAPQGLKIACGENPKRVYGGKGRSPATRM
                     GNVAGYRKAWIDAQDYARRWDKWEKGGGTGEAPKRDLQLETLAGVLKGEILVQNHCYR
                     ADEMANMIDISREFGFRIRTFHHANEAYKIAPLLAKEDICVATWASWWGYKMEVFDAI
                     EENAALVHAAGGCAIIHSDDPIVIQRLNQEAAAALSAAWRAGIRISKADAIRWFTANP
                     AKALGIADRVGTLEPGKNADVVLWSHDPFSIYARAEKVWIDGGIVFDRADPSYRRHSD
                     FLLGQPGATR"
     gene            1206..>1762
                     /locus_tag="VN034_08745"
     CDS             1206..>1762
                     /locus_tag="VN034_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV60727.1"
                     /translation="MTRRLTAFAALLLATPASAETIAIVDARLMTMTGAGEIGRGTIV
                     IRGGKVAALGVDVPVPADARVIDGRGGVVTPGFIQADSTLGAVEVSQVPASADRATHS
                     KTISAAFDISQGLNPDSILLPVSRLAGITHAVTTPLYDDRGGRSLQFAGQAAVIDLAQ
                     RPQMVTRPRAAMVLEMGEGGAERAGG"
BASE COUNT          310 a          559 c          631 g          262 t
ORIGIN      
        1 ggcgcggaga tcgcgaacgg gacggtgctg atggaggacg gcaagatcgc cgccgtcggc
       61 gccgcgctcg cggtgccggg cggctatcga accgtggacg cgcgcggcaa gtgggtgacc
      121 ccgggggtga tcgacgcgca ctcgcatctc ggcgcatggg cgcagcccga atcggtcgag
      181 atgcacaacg acgtcaacga gatgaccgat cccaacaccg cgcaggtctg gatcgaacat
      241 tcggtctggc cgcaggaccc gggtttcgac gcggcgcgcg aggcgggggt cacgacgatg
      301 atggtcctgc ccggttcggg caatttgttc ggcgggcgca cggtgacgct caagaatgtg
      361 ccgtcggtaa cgatgcaggg gatgaagttc cccggtgcgc cccagggcct gaagatcgcg
      421 tgcggcgaga atcccaagcg cgtctatggc ggcaagggac gctcgccggc gacgcggatg
      481 ggcaatgtcg cgggctatcg caaagcctgg atcgacgcgc aggactatgc gcgtcgctgg
      541 gataagtggg agaagggcgg cgggaccggc gaggcgccga aacgcgacct gcagctggaa
      601 accctcgctg gcgtgctgaa gggcgaaatc ctcgtccaga accactgcta ccgcgccgac
      661 gagatggcga acatgatcga tatctcgcgc gaattcgggt tcaggatccg cacctttcat
      721 cacgccaacg aagcctacaa gatcgcgcct ttgcttgcga aggaggatat ctgcgtcgcg
      781 acatgggcca gttggtgggg gtataagatg gaggtgttcg acgcaatcga ggagaatgcg
      841 gcgctcgtcc acgcggcggg cggctgcgca atcatccatt cggacgatcc gatcgtcatc
      901 cagcggttga accaggaggc ggcggcggcg ctgtcggcag cgtggcgtgc gggcatccgg
      961 atcagcaagg ccgacgcgat ccgctggttc accgccaatc cggcaaaggc gctcggcatc
     1021 gccgaccgcg tcggtacgct cgaacccggc aagaacgccg acgtcgtgct ttggagccac
     1081 gatccgttca gcatctatgc gcgcgccgaa aaggtctgga tcgacggcgg gatcgtcttc
     1141 gaccgcgcgg acccttccta tcgccgccat tcggacttcc tgctcggcca accgggagcg
     1201 acacgatgac ccgccgcctg accgccttcg cggcactgct tctggcgacc ccagcctcgg
     1261 cggaaacgat cgcgatcgtc gatgcgcggc tgatgacgat gacgggcgcc ggcgagatcg
     1321 ggcgcggaac gatcgtgatc cggggcggaa aggtcgccgc gctcggcgtc gacgtcccgg
     1381 ttcccgccga cgcgcgcgtg atcgatggcc gcggcggggt cgtgacgccg ggcttcatcc
     1441 aggcggacag caccttgggg gcggtcgagg tcagccaggt cccggcgagc gccgatcgcg
     1501 cgacgcacag caagacgatc agcgcagcct tcgacatctc gcaggggctc aaccccgatt
     1561 cgattctgct tccggtatcg cggctcgcgg ggatcaccca cgcggtcacg acgccgctct
     1621 acgacgatcg cggaggccgc tccttgcaat tcgccggaca ggccgcggtg atcgatctcg
     1681 cgcagcgccc gcaaatggtg acgcggccgc gcgccgcgat ggtgctcgaa atgggcgagg
     1741 gcggtgccga acgcgccggc gg
//
LOCUS       DATMSU010000318         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_71887, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000318 DATMSU010000000
VERSION     DATMSU010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71887"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VN034_08750"
     CDS             complement(<1..201)
                     /locus_tag="VN034_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60728.1"
                     /translation="MSESNKMAPDVTGGTTGGMTLTNRAILIAAFILLAAAVGYAIWR
                     DSAPSATGPASESAAAPSDQLAG"
     gene            358..1713
                     /gene="gdhA"
                     /locus_tag="VN034_08755"
     CDS             358..1713
                     /gene="gdhA"
                     /locus_tag="VN034_08755"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HWV60729.1"
                     /translation="MAVSDHVDFSTFMEGVKRRNPGQPEFVQAVQEVSEGIFDFIADK
                     EEYHAQQILRRIAEPDRVVSFRVCWEDDNGNIRVQRGWRVQNNNAIGPYKGGIRFHPS
                     VTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHI
                     GADVDVPAGDIGVGGREIGFMFGQYKRITNEFTGVLTGKGLEWGGSLIRTEATGYGAV
                     YFLANMLAAKGQDLVGKSAVISGSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGI
                     TQDKIDWVKAHKTHRRGRIEEYVDEFKGATFTPGKTPWGVKCDVALPCATQNELLGDD
                     AKTLVKNGCIAVSEGANMPTNLDGVHVFKDAKIMFAPGKAANAGGVAVSGLEMSQNSG
                     RRAWSEGELQQMLKDIMDGIHARCLTYGDQGNGYIDYVKGANIAGFKKVADAMLAFGV
                     V"
     gene            1764..3197
                     /locus_tag="VN034_08760"
     CDS             1764..3197
                     /locus_tag="VN034_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWV60730.1"
                     /translation="MTAHAGKERGDRPWTAFAVLAAMLLALAIAAFVFAAPARSQGES
                     GARYTGVASCAGSTCHGRMEGDGTIVRQDELMKWQEPSTPGGAHSRAWAVLNNNRSQF
                     IARNLGIGDPAKAQMCLGCHSTAGTARAVPAEDGVGCESCHGPAGGWLASHYAGVGTN
                     ADPDREMRDKHLANLSAGLKKLEDPVVRAGVCVDCHFGSAADGQFVTHRIMAAGHPRI
                     SFELDLFSSLQAHHQEDADYGWRKFGAAGRRTDHVQMWAVGQATAIERSLALFQTRRG
                     TEGIFPEFTFLDCHSCHRRIFDQAKPVRTGLDNPGRNLPEGMPAYNDENLIMLAAAAR
                     MASPALADQLAARTAAFHKAMAADRPSAVQAAAQLSQTVAALKSAFASRNFSGADAFA
                     MVDAISAKAISDRYTDYSGSQQAVMGVDTLLNAMVSSGRVTVGAAAGIRGDIDRAYAA
                     VKDPNAYKPSEFQTSFGSAVRAIGALR"
     gene            3259..>3470
                     /locus_tag="VN034_08765"
     CDS             3259..>3470
                     /locus_tag="VN034_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60731.1"
                     /translation="MLTSCGGGDGGGGTPTPTPTPTPTPTPTGSVFQIPALTNLTTGD
                     IERIIAQGVAEAQAQNFRAGFVVIDRV"
BASE COUNT          614 a         1101 c         1178 g          577 t
ORIGIN      
        1 cccggcgagc tggtcggacg gcgcggcggc gctttcgctg gccggtcccg tggcggacgg
       61 agcggaatcg cgccagatcg catagccgac cgccgccgcg agcaggatga aggcggcgat
      121 caatatcgcg cgattggtga gcgtcatgcc gccggtcgtg ccccccgtca cgtctggcgc
      181 catcttgttg ctctcgctca tttttgcccc tcttttacgc cgcatgaaaa atgccggctg
      241 tgcgcgccgt catattgcct ttgcccggcg atggtgtagc ctttggcata taaggtcggt
      301 caacgataaa atcgtcaggg gtcgcaccgg ctttcccaat caacagggga ggggtgcatg
      361 gcagtttcgg atcacgtcga tttttcgacg ttcatggaag gcgtgaagcg gcgcaatccg
      421 gggcaacccg agttcgtcca ggcggtgcag gaagtgtcgg agggcatttt cgacttcatc
      481 gccgacaagg aagaatatca cgcgcagcag atattgcggc gtatcgccga gcccgatcgg
      541 gtggtgtcgt tccgcgtctg ctgggaggac gacaacggca atatccgcgt ccagcgcggc
      601 tggcgcgtcc agaacaacaa tgcgatcggc ccctataaag gcggtatccg ctttcacccg
      661 agcgtcaccg aaagcgtgct caaattcctc gcgttcgagc agacgttcaa gaacgcgctg
      721 accggcctcc ccatgggcgg cggcaagggc gggtcgaact tcaaccccaa gggcaagagc
      781 gtgcgcgaga tcatgcgctt ttgccagagc ttcatgaccg aactctatcg ccacatcggc
      841 gccgacgtcg acgtgcccgc gggcgacatc ggcgtcggcg ggcgcgagat cggcttcatg
      901 ttcggccagt ataagcggat caccaacgaa ttcaccgggg tgctcaccgg caaggggctc
      961 gaatggggcg gctcgctgat ccgcaccgag gcgacgggct atggcgcggt ctatttcctc
     1021 gccaacatgc tcgccgcgaa ggggcaggac ctggtcggca agagtgcggt catttcgggg
     1081 tcgggcaatg tcgcgacgca tgcggccgag aagatcgtcc agctcggcgg caaggtgctg
     1141 acgctgtccg attcgggcgg cttcatccac gatcccgacg gcatcacgca ggacaagatc
     1201 gactgggtga aggcgcacaa gacgcatcgc cgcgggcgga tcgaggaata tgtggacgag
     1261 ttcaaaggcg cgaccttcac acccggcaag acgccgtggg gggtgaagtg cgacgtcgcg
     1321 ctgccgtgcg cgacgcagaa cgaactgctc ggcgacgatg cgaagacgct cgtcaagaat
     1381 ggctgcatcg ccgtcagcga aggcgccaac atgccgacca acctcgacgg cgtgcatgtg
     1441 ttcaaggatg cgaagatcat gttcgcgccg ggcaaggcgg cgaatgccgg cggcgtcgcg
     1501 gtgtcggggc tcgagatgag ccagaacagc ggccgccgcg cgtggagcga gggcgagctt
     1561 caacagatgc tcaaggatat catggacggt atccatgcgc gctgcctgac ctatggcgat
     1621 cagggcaatg gctatatcga ctatgtgaag ggcgccaata tcgcgggatt caagaaggtt
     1681 gccgacgcga tgctggcttt cggggtggtg taaatcgatc cagggcgtaa taagcgccgg
     1741 gttgggaaaa aggggtactg cggatgacgg cgcacgctgg gaaggagcgc ggcgatcggc
     1801 cttggacggc atttgccgtt ctggcggcga tgctgctcgc gctcgccatt gcggcgttcg
     1861 tcttcgcggc gcccgcgcgc tcgcagggcg aaagcggcgc acgctatacc ggcgtcgcct
     1921 cgtgcgcggg gtcgacctgt cacggccgga tggaaggcga cggcactatc gtccgccagg
     1981 acgagctgat gaagtggcag gagccttcga cccccggcgg tgcgcacagc cgcgcctggg
     2041 cggtcctcaa taacaatcgc agccagttca tcgcgcgcaa cctcggcatc ggcgaccccg
     2101 ccaaggcgca gatgtgcctc ggctgccaca gcaccgcggg caccgcacgc gcggttccgg
     2161 ccgaggatgg cgtcggctgc gaatcgtgtc acggtcccgc gggcggctgg ctcgcgagcc
     2221 attatgccgg ggtgggcacc aacgccgatc ccgaccgcga aatgcgcgac aagcatctcg
     2281 ccaacctgtc ggcgggcctc aagaagctcg aggatccggt ggtccgcgcc ggcgtgtgcg
     2341 tcgattgcca tttcggttcg gccgccgacg gccagttcgt cacccaccgc atcatggcgg
     2401 cggggcaccc gcgcatttcg ttcgagctcg acctcttctc gtcgctgcag gcgcatcatc
     2461 aggaggatgc cgactatggc tggcgcaagt tcggcgcggc gggccggcgc accgaccatg
     2521 tccagatgtg ggcggtggga caggcgaccg cgatcgagcg cagcctcgcg ctgttccaga
     2581 cgcggcgcgg gaccgagggg atctttcccg aattcacctt cctcgactgt cacagctgcc
     2641 atcgccgcat cttcgaccag gcgaagccgg tgcgcacggg gctcgacaat ccgggccgca
     2701 atcttcccga gggcatgccg gcctataatg acgagaatct gatcatgctc gccgcggcgg
     2761 cgcgcatggc ttcgcccgcg ctcgccgacc agctcgcggc gcgcaccgcg gcctttcaca
     2821 aggcgatggc cgccgaccga ccgagcgcag tgcaggccgc ggcgcagctg tcgcagacgg
     2881 tcgcggcgct gaaatcggcc ttcgcctcgc gcaatttctc gggcgccgat gccttcgcga
     2941 tggtcgatgc gatttcggcg aaggcgatca gcgaccgcta caccgactat tcgggatcgc
     3001 agcaggcggt gatgggggtc gataccctgc tcaacgcgat ggtgtcgtcg ggacgcgtca
     3061 cggtcggcgc ggcggcggga atccgcggcg acatcgatcg tgcctatgcc gcggtcaagg
     3121 accccaatgc ctataagccg tccgaattcc agacctcgtt cggcagcgcg gtgcgcgcga
     3181 tcggggcgct gcggtaagat catgcgaagc aagacctctc ttttccgttc ggccgccctc
     3241 gctgcgatgg gcgcattgtt gctgacgtca tgcggcggcg gcgatggcgg cggcggcacg
     3301 ccgaccccca ccccgacccc cacgccgaca ccgacaccga cgggcagcgt tttccagatc
     3361 cccgcgctga ccaacctcac caccggcgat atcgaaagga tcatcgcgca gggagtcgcc
     3421 gaggcgcagg cgcagaattt ccgcgccggc ttcgtcgtca tcgaccgggt
//
LOCUS       DATMSU010000319         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_90983, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000319 DATMSU010000000
VERSION     DATMSU010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90983"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2715
                     /locus_tag="VN034_08770"
     CDS             <1..2715
                     /locus_tag="VN034_08770"
                     /inference="COORDINATES: protein motif:HMM:NF027309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HWV60732.1"
                     /translation="FAGDVERVSGVRPDVSQAAPEGRLAILAGTIGGSPVIDRLVAEG
                     RLTVDAVKGQSEAYTIAVVDKPSPGVARALVVAGADRRGTAYGIFRLSEQIGVSPWIW
                     WAEAAPDRRSTLTLDAGTIVQGSPSVRYRGIFLNDEDWSLRPWATANDPSGDIGPATH
                     ARIFELLLRLRANFLWPAMHKVTSAFNQVQGNAEMADRYAIVMGASHAEPMLRNNVAE
                     WNFEQRGEFNYAKNRLNIVQYWRDRVRANHQYENVYTVGMRGIHDSPAEMNRGSDGVR
                     LLEQVVTDQRELLKEVGKDPAAVPQVFVPYKEVLDIYRKGMTVPDDVTLGWVDDNYGY
                     IRQLSTKAEQKRSGGAGVYYHISYWGVPNDYLWLDSTPPALIGEEMGKAFATNARRLW
                     VLNVGDLKPGEKGLNYFMDLAYDYEGTSKLGQRGWLRRWAETTFGPEQADAIAALLGD
                     YYRLNFARKPEHMGWNTAETAPRPTEFSPVAYGDEAGIRAAEFRALDQRAEAISAKLP
                     AEKREGFYHLILYPVRGSALMNAKMLSADRSFLYAHQGRASANLYADAAAEAYRGIKK
                     ATAVYNATGDGKWAQFMHDEPRYQSVFNMPPVGRVTPLEQPGLGVAVEGAIDAWTQQP
                     ARIFEAADTALRVERWRTQGASNMRLPVFERATDRRYFIDAFNTGRGALDIRAAGSAP
                     WIEIEREAKPGGDQRLWVSIDWSKLKAGASAAGTVTVSAAGASRSIAVEARNVAAPKG
                     MLVEDNRIIAFNASRYAALQPGWQRMPDLGRSGDAIATSLELPSATDPAKTPYVEYRF
                     RTTSSGAAKLRATLLPSFALSAENRLRYGVSIDGGPIRVVDADKRDWGDAVERNAMTS
                     VTDWTIARAGEHRLRVHALDPGVVLDSFVIDFGGLARSYLAPPETIAR"
     gene            complement(2761..3510)
                     /locus_tag="VN034_08775"
     CDS             complement(2761..3510)
                     /locus_tag="VN034_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWV60733.1"
                     /translation="MAPNQKLYQRIADSIAAEIASGAYEPGARLPAERDLAEQFKVSR
                     PTIREAMIALEIRGLVGAKKGSGIYVSTTPPETALIAAELDVGAFELIEARTLFESEA
                     AALAAAAIDAPQLAELRALLGEMKQERDTDAAFDADKRFHIAIAAATGNSLIISVIEM
                     LWAVREQSPLCVHMFARARRKGVTPRANEHELIVDALAQHDPQRARDAMRAHLRRVTD
                     DLLDATKLELMEKAEADFAEQLRRVAARNAV"
     gene            complement(3666..>4084)
                     /locus_tag="VN034_08780"
     CDS             complement(3666..>4084)
                     /locus_tag="VN034_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWV60734.1"
                     /translation="SRSALRTYDPVDAADAHSARPTVAGLFFPVITMAEPAAHAHSKR
                     ELLGTDARPERVARFSAERDLPPDMPPTLVAHAANDKVVSPANSLMMFAALQAARIPS
                     ELHIFETGGHGLPLREKGEEHPWPALFERFARRHGL"
BASE COUNT          702 a         1304 c         1391 g          687 t
ORIGIN      
        1 tttgcgggcg atgtcgagcg cgtctccggc gttcgtcccg acgtgtcgca ggcggcgccc
       61 gaagggcggc tcgcgatcct cgccgggaca atcggcgggt cgcccgtgat cgaccggttg
      121 gttgccgaag gacggctgac cgtggacgct gtcaaagggc agagcgaggc ttatacgatc
      181 gcggtcgtcg acaagccgtc gcccggcgtt gcgcgcgcgc tggtcgtcgc cggcgccgac
      241 cggcgcggca ccgcctatgg catcttccgc ctgtcggaac agatcggcgt atcgccatgg
      301 atatggtggg cggaggccgc gcccgaccgc cgctccacgc tgacgctcga tgccggaacg
      361 atcgttcagg ggtcgccctc ggtccgctat cgcggcattt tcctgaacga cgaggattgg
      421 tcgctgcggc cctgggcaac cgcgaacgac ccgagcggcg atatcggacc cgcgacgcac
      481 gcgcgtatct tcgagctgtt gctgcgcctg cgtgccaact ttctctggcc cgcgatgcac
      541 aaggtcacct cggcgttcaa tcaggtgcag ggcaatgccg aaatggcgga ccgctatgcg
      601 atcgtgatgg gcgcaagcca tgccgaaccg atgctgcgga acaatgtcgc cgagtggaat
      661 ttcgaacagc gcggtgagtt caactacgcg aagaaccggc tcaatatcgt acaatattgg
      721 cgcgatcggg tgcgcgccaa tcatcagtac gagaatgtct acaccgtcgg catgcggggc
      781 atccatgaca gcccggcgga aatgaaccgt ggcagcgacg gcgtacggct gctcgagcag
      841 gtggtcaccg accagcggga attgctcaag gaagtgggca aggatccggc ggccgtcccg
      901 caggtcttcg tgccctacaa ggaagtgctc gatatctatc gcaagggcat gacggtgccc
      961 gacgatgtga cgctgggctg ggtcgacgac aattatggct atatccgcca actctcgacc
     1021 aaggccgagc agaagcggtc gggcggcgcg ggtgtctatt accacatctc ctattggggc
     1081 gtgcccaacg actatctatg gctcgacagc accccgcccg cgctgatcgg cgaggaaatg
     1141 ggcaaggcgt tcgccaccaa cgcgcggcgg ctgtgggtgc ttaatgtcgg ggacctcaaa
     1201 ccgggcgaaa agggtctcaa ttacttcatg gacctcgcct atgattacga aggcacctcg
     1261 aaactggggc agcgcggatg gctcaggcgc tgggccgaga cgacgttcgg ccccgaacag
     1321 gccgacgcca tcgcggcctt gctgggggac tattaccggc tcaatttcgc gcgcaagccc
     1381 gaacatatgg gttggaacac cgccgaaacc gcaccgcgcc cgaccgagtt ctcgcccgtc
     1441 gcctatggcg acgaagcggg cattcgcgcg gccgagttcc gggcgctcga ccagcgcgcc
     1501 gaggcgatca gcgcaaaact gcccgccgaa aagcgcgagg gcttctatca tctgatactc
     1561 tacccggtgc gcgggagcgc gctgatgaac gccaagatgc tgagcgccga ccgcagcttc
     1621 ctctatgcgc atcagggccg cgccagcgcc aatctctatg ccgatgccgc cgccgaggcc
     1681 tatcgcggga tcaagaaggc gaccgccgtc tataatgcga cgggcgacgg caaatgggcg
     1741 cagttcatgc acgacgagcc ccgctaccag tcggtgttca acatgccgcc ggtcggccgg
     1801 gttacgccgc ttgaacagcc ggggctgggc gttgccgtcg aaggcgcgat cgacgcatgg
     1861 acgcagcagc cggctcgaat attcgaagct gccgacaccg cgctgcgggt cgaacgctgg
     1921 cgcacccaag gcgcgtcgaa tatgcggctg ccggtgttcg aacgcgccac cgaccgccgc
     1981 tatttcatcg acgcgttcaa caccggtcgc ggtgcgctcg acatccgtgc cgcgggatcc
     2041 gcgccgtgga tcgagataga gcgtgaggcg aagccgggcg gcgatcagcg gctgtgggtc
     2101 tcgatcgact ggagcaagct gaaggccggt gcgagcgccg ccggcacggt cacggtctcg
     2161 gctgcgggag cgagccgcag catcgcggtc gaggcgcgca atgtcgctgc accaaaaggc
     2221 atgctggtcg aggacaatcg catcatcgcc ttcaacgcca gccgctatgc cgccctccag
     2281 ccgggttggc agcgcatgcc cgatctgggg cgttcgggcg atgccatcgc gaccagtctt
     2341 gaattgccga gcgcgaccga tccggcaaag acgccctatg tcgaatatcg gtttcgcacg
     2401 accagtagcg gcgccgcgaa gctgcgtgcg acgctgctcc ccagcttcgc gctcagcgcg
     2461 gaaaacaggc tgcgttacgg cgtctcgatc gacggcgggc cgatcagagt ggtcgatgcc
     2521 gacaagcgcg actggggcga tgcggtcgag cgcaatgcga tgacctcggt caccgactgg
     2581 acgattgcca gggcgggcga gcaccggctt cgcgtccatg ccctcgatcc cggggtggtg
     2641 ttggattcgt tcgtgatcga tttcggcggg ctcgcccggt cctatctggc gccccccgaa
     2701 acgatcgcgc gctgacggac ccgcgtcccg cgccggatcg gcgcggggct aggtcacgac
     2761 ctaaaccgcg tttcgcgcgg cgacgcggcg aagctgctcg gcaaaatccg cctcggcctt
     2821 ttccatcagc tccagcttgg tcgcgtcgag caggtcgtcg gttacccggc gcaaatgcgc
     2881 ccgcatcgca tcccgcgccc gctgcgggtc atgctgcgcg agcgcgtcga cgatcagttc
     2941 atgctcgttg gcccgcgggg tgacgccttt gcgccgggcc cgggcgaaca tgtggacgca
     3001 gagcggcgat tgttcgcgca ccgcccacag catctcgatc accgatatga tcaggctgtt
     3061 gccggtggcg gccgcgatgg cgatgtgaaa gcgcttgtcg gcgtcgaacg ccgcatcggt
     3121 gtcgcgctcc tgcttcatct caccaaggag cgcgcgaagc tcggcaagct gcggcgcgtc
     3181 gatcgccgcc gcggcgagcg ccgccgcctc gctttcgaac aaggtgcgcg cttcgatcag
     3241 ttcgaacgcg ccgacatcca gttccgcggc gatcagcgcg gtctcgggcg gggtggtgct
     3301 gacatagatg ccagaccctt ttttcgcacc gaccagacca cggatttcga gcgcgatcat
     3361 cgcttcgcgg atcgtcggcc ggctcacctt gaactgttcg gcgagatcgc gttcggcggg
     3421 cagccgggcc ccgggttcat aggcgccgct ggcgatttcg gcagcgatgg aatcggcgat
     3481 gcgctgataa agcttttgat tgggtgccat ggtgtgatgc ctgatgaatg tcgcatctga
     3541 ttgacggata gatggcttgg cgtcaatcac cctcgaaaat tggccgggcc aatgatgtcg
     3601 tttgctccga cgaatggtaa ggccggaaga ttgtcgcaac tccggtcgcg cgatcgtcgc
     3661 gcgcgttaca gcccgtggcg gcgggcgaag cgctcgaaca gcgccggcca gggatgctct
     3721 tctcccttct cgcggagcgg caggccgtgg ccgccggtct cgaaaatatg cagctcggac
     3781 gggattcgcg cggcctggag cgcggcgaac atcatcaggc tgttggcggg gctgacgacc
     3841 ttgtcgttcg ccgcgtgggc gacgagggtt ggcggcatgt cggggggcag atcgcgctcg
     3901 gcggaaaagc gcgcgacgcg ttcggggcgg gcatccgtcc ccaacaactc gcgcttcgaa
     3961 tgcgcgtgcg cggcgggctc cgccatggtg atgaccggga agaacagccc cgccaccgtc
     4021 ggacgcgccg agtgcgcatc ggcggcgtcg accggatcat aggtgcgcag cgccgaccgg
     4081 ctgg
//
LOCUS       DATMSU010000320         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_5995, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000320 DATMSU010000000
VERSION     DATMSU010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5995"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..443)
                     /locus_tag="VN034_08785"
     CDS             complement(6..443)
                     /locus_tag="VN034_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWV60735.1"
                     /translation="MRLTRYTDYAMRVLLYVGAREEERPSPISEISRAYGISQNHLMK
                     VVNDLVNAGYLESVRGRFGGIRLARPAAEINVGAVVRHTEEGFDLVDCGSCIIAPACG
                     LTGALAEALAAFMTVLDGYSLADLLAKRANLAALFGVPEVSKG"
     gene            545..940
                     /locus_tag="VN034_08790"
     CDS             545..940
                     /locus_tag="VN034_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HWV60736.1"
                     /translation="MNHDDQIDEAALARLIPAFYDRVRTDAEIGPLFNEAIEDWPAHL
                     EKLVAFWSSVMLTSGRYKGSPVAEHLKHKARITPAMFDRWLAIWTATTDELLAPAAAA
                     ALQAKAARIAESLQLALFFRLDDPGRRAA"
     gene            976..1425
                     /locus_tag="VN034_08795"
     CDS             976..1425
                     /locus_tag="VN034_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HWV60737.1"
                     /translation="MRTASAHAMAIVKATAPALEKHGLAITTAMYARLFENEEIAAMF
                     DDAAQTSGEQPKRLAAAILAYAKNIDKLQNLGPAVSRMVARHIETGVRPEHYPHVANA
                     LLPAIRDVLGKEVATDEVLAAWGEAYWMLADILIAAEAQAYEDAEAA"
     gene            complement(1695..2027)
                     /gene="grxD"
                     /locus_tag="VN034_08800"
     CDS             complement(1695..2027)
                     /gene="grxD"
                     /locus_tag="VN034_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HWV60738.1"
                     /translation="MTDPTTHDRITKLVADNPVLLFMKGTPLFPQCGFSSRAIAMLDR
                     LGIAYETVDVLQDMEVRQGIKEFSDWPTIPQLYVKGEFVGGSDIMMEMWEAGELHTLM
                     DGIPTRAE"
     gene            complement(2077..2310)
                     /locus_tag="VN034_08805"
     CDS             complement(2077..2310)
                     /locus_tag="VN034_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HWV60739.1"
                     /translation="MGMREDDLRAMIVAAFPDAEVTITDLAGDNDHYAAHVVSASFQG
                     MTRVAQHKAVYAALGGRMGGELHALQLTTAIPS"
     gene            complement(2321..2647)
                     /locus_tag="VN034_08810"
     CDS             complement(2321..2647)
                     /locus_tag="VN034_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HWV60740.1"
                     /translation="MSAFDDRERAFETKFARDQEVQFRITARRNRLLGEWAAERMGLT
                     PEETDAYAKAVVQADFEEAGDEDVIRKLAGDLTAASVEISDAEIRAALNDKTAEARRQ
                     FIDSQN"
     gene            2857..>3152
                     /locus_tag="VN034_08815"
     CDS             2857..>3152
                     /locus_tag="VN034_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60741.1"
                     /translation="MTVRRPHFAPVLAALVLAAPGTALAREGDPTLRDSFAIGEQGGS
                     LCEVQATVHDRVIEGMFDRAWTIICRDASQPVGTVRALRADADTARARIERARAG"
BASE COUNT          560 a         1049 c          972 g          571 t
ORIGIN      
        1 gcttttcagc ccttcgaaac ttccggcaca ccgaacagcg cggccagatt cgcgcgcttc
       61 gcgagcaggt cggcgaggct gtagccatcg agcaccgtca tgaacgcggc gagcgcctcg
      121 gccagcgcgc ccgtcagccc gcatgcgggc gcgatgatgc agctgccgca atcgacgagg
      181 tcgaaaccct cctcggtatg gcgcaccacg gcgccgacat tgatctcggc cgcgggtctg
      241 gcgagccgga tgcccccgaa acgcccgcgc acgctttcga gatagcccgc gttcaccagg
      301 tcattgacca ccttcatcag atgattctgc gaaatgccat aggcgcgcga aatctccgag
      361 atcgggctcg gccgttcttc ctcccgcgcc cccacataga ggagcacgcg catcgcataa
      421 tcggtgtagc gcgtcaggcg catcgcaatc cttcaaatcc gcttcgaccg cataatatgt
      481 attttcgttg catgtttcaa atttcgaaca tacatgcgca aaaaatacat gattggagcg
      541 agtcgtgaac cacgacgatc aaatcgacga agccgcgctc gcgcgcctga ttccggcctt
      601 ttacgaccgg gtgcgcaccg atgccgagat cggcccattg ttcaacgaag ccatcgaaga
      661 ctggcccgcg catctcgaaa agctcgtcgc cttctggtcg tcggtgatgc tgacgagcgg
      721 ccgctacaag ggtagcccgg ttgccgagca tctgaagcac aaggcgcgca tcaccccggc
      781 gatgttcgac cgatggctcg ccatctggac cgcgacgacc gacgaattgc tggcgcccgc
      841 cgcggccgca gccctgcaag ccaaggccgc acgcatcgcc gaaagccttc agcttgccct
      901 cttcttccgc ctcgacgacc ccggtcgccg ggcggcctga agccttcctc ccctcctcga
      961 ttaaaaggaa atcctatgcg caccgcgtcc gcccacgcca tggcgatcgt caaagccacc
     1021 gcccccgcgc tcgaaaagca cggccttgcg atcaccaccg cgatgtatgc ccggcttttc
     1081 gagaatgagg agatcgcagc gatgttcgac gacgccgcgc agacgagcgg cgaacagccg
     1141 aaacggctcg ccgcggcgat cctcgcttat gcgaagaata tcgacaagct ccagaacctc
     1201 ggtccggcgg tgtcgcgcat ggtcgcccgc catatcgaga ccggcgtccg gcccgaacat
     1261 tatccgcacg tcgccaacgc gctgctcccc gcgatccgcg acgtgctggg caaagaggtc
     1321 gcgaccgacg aggtgcttgc ggcatggggc gaggcctatt ggatgctcgc cgacatccta
     1381 atcgccgccg aggcgcaagc ctatgaggat gccgaggcgg cctgaacgaa gggtcgggtt
     1441 cggccgttag cggacatcga gagaagaaag cccctcccct tcaggggagg ggttggggtg
     1501 ggggcccgaa accttgcgcg aggccgatag ccccacccca ctgcgactag gcagcaagct
     1561 gccaagtctt cgttgcccct cccctcaagg ggaggggctt gatggatcgc agcgtacgct
     1621 ccccacccca aagcggacaa acaaaagccc gccggagcca ggcaccagcg ggcttttgtt
     1681 ttcagaaacg ccccttattc ggcgcgcgtc gggatgccgt ccatcagcgt gtgcagttcg
     1741 cccgcttccc acatttccat catgatgtcg ctgccgccga cgaattcgcc cttcacatag
     1801 agctggggga tggtcggcca gtccgaaaat tccttgatgc cttgccggac ttccatgtcc
     1861 tgcagcacat cgaccgtctc atatgcgata ccgaggcggt cgagcatcgc gatcgcgcgc
     1921 gaggaaaaac cgcactgggg aaagagcggc gtgcccttca tgaagagcag cacggggttg
     1981 tcggcgacca gcttcgtaat gcggtcgtgg gtggtcgggt cggtcattgt cggtctccgg
     2041 tcaggcgcgc catcgcgccc gcaaatcatc tgccccctat gaggggatcg cggtcgtcaa
     2101 ttgcaaggca tgaagttcgc cgcccatgcg tcctcccaag gcggcataga ccgccttgtg
     2161 ctgcgcgacg cgggtcattc cctgaaagga tgcgctgacg acatgcgccg cataatggtc
     2221 gttgtcgccg gcgaggtcgg tgatcgtcac ctccgcatcg ggaaaggccg cgacgatcat
     2281 cgcccgcaga tcatcctcgc gcatgcccat gacgatcgcc tcagttctgg ctgtcgatga
     2341 actggcgccg ggcttcggcg gtcttgtcgt tcagcgcggc acggatttcg gcgtcgctga
     2401 tctcgacgct cgccgcggtc agatcgcccg cgagcttgcg gatgacgtct tcgtcgcccg
     2461 cttcctcgaa atcggcctgc accaccgcct tggcataggc gtcggtttcc tcgggggtca
     2521 gccccatgcg ttcggccgcc cattcaccga gcagccggtt acggcgcgcg gtgatccgga
     2581 actgcacctc ctggtcgcgt gcgaatttcg tttcgaaagc ccgttcccga tcgtcgaatg
     2641 cgctcatctt gtcctcgtgc gaatgaatct tccttgcaga taggtcggcg accccggcgg
     2701 cgcaagccgc gtgtgcggca gatcacttcc ctttcgctta cgaagggttt agggtttgtc
     2761 gtcaagggaa tgggtcgtac cgcagcgatg aagactgcgc cttgccggca tgacgtcggc
     2821 acggcgcctt cgcgcttgtg cttgggggat tgggtgatga ccgttcgacg tccgcatttc
     2881 gcgcccgttc tggctgcgct ggtcttggcc gcgccgggaa ccgcgctggc gcgggaaggc
     2941 gacccgacgc tccgcgacag ttttgcgatc ggcgagcagg gcggctcgct gtgcgaggtg
     3001 caggctaccg tccatgaccg cgtgatcgag ggcatgttcg accgcgcctg gacgatcatc
     3061 tgccgcgatg ccagccagcc cgtcggcacg gtgcgcgcgc tgcgcgccga cgccgatacg
     3121 gcgcgcgcac ggatcgagcg cgcacgggcg gg
//
LOCUS       DATMSU010000321         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_24254, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000321 DATMSU010000000
VERSION     DATMSU010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24254"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN034_08820"
     CDS             <1..343
                     /locus_tag="VN034_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWV60742.1"
                     /translation="EWEAAAAGIDPRTGVQLDGAGAVAPRYVEQDGRLASMFGNVWEW
                     TGSAYRPYPGYRPAEGAVGEYNGKFMSGQFVLKSGSCATPRGHARASYRNFFYPQQRW
                     QFTGVRLAKDL"
     gene            346..1332
                     /gene="egtD"
                     /locus_tag="VN034_08825"
     CDS             346..1332
                     /gene="egtD"
                     /locus_tag="VN034_08825"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWV60743.1"
                     /translation="MLEMAQSVTRPREARPVDPLFRAEVLLGLGQAQKTIPARWLYDR
                     RGSELFEDITRLEEYYPSRTETALLRHHGRDIALHCGPGRVVVEFGSGSSAKTPLLLE
                     AVDPSAYVAIDICGEFLRHACDELAGTFDELEIWPVEANFMAPVALPPRLAGQRTLGF
                     FPGSTIGNMLPADAVDLLRMMRHTLGERAQLLIGIDRTKAPSRLLPAYDDARGVTAAF
                     NLNLLDRINDELGGDIPVARFRHEARWNADWQRIEMHLAATGPVSFSVAGRRFAMLAG
                     ETIHTENSHKYAPEQAGLLLRAGGWSPVQQWTDGNGDFLLLLTEATPSDLSP"
     gene            complement(1488..1781)
                     /locus_tag="VN034_08830"
     CDS             complement(1488..1781)
                     /locus_tag="VN034_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60744.1"
                     /translation="MPPAETIALSRSEWHAVRDLLDRADARKDGPLRRIASIFRAPAK
                     ERRGPAAEADLVDAVSAFVCGARSRSRDEEYGARLVELGLSSMQVAAIASLSS"
     gene            complement(2167..2877)
                     /locus_tag="VN034_08835"
     CDS             complement(2167..2877)
                     /locus_tag="VN034_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWV60745.1"
                     /translation="MPDAHLASVRWTAAVRLIESKYPPIDLFEDLADPEDWELLAAAE
                     ARTNPRIAETIGNLDLVPAARRVAGPGASYVMAPFTHCSPDRPGRFHDGSFGAYYAAR
                     DFATAVAEVTHHQARRLADSRDEPGWISDMRELVGRVRAELVDIRDGDFGLLLAPDDY
                     APSQAFARQHRDAGASGIVYPSVRNRGGECIAAFWPDVVARPVQKRHFRYHWDGQRID
                     MIHELRLDGKGPVFRLEV"
     gene            complement(2885..3223)
                     /locus_tag="VN034_08840"
     CDS             complement(2885..3223)
                     /locus_tag="VN034_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HWV60746.1"
                     /translation="MQRAVIRLFGHWGVTDAQAAILLGDIAVRTYQRWKQGEYGRWTV
                     DLAARLSNLIGIHKALRLLYTETERGYRWINAPNRAFGDRSALDVMLGGQLTDLMRVR
                     RYLDSYRGAW"
     gene            3338..>3808
                     /locus_tag="VN034_08845"
     CDS             3338..>3808
                     /locus_tag="VN034_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60747.1"
                     /translation="MPIVKKTPIMTAAEAGPARTSPASPVSPDRMPPARYFRAVALAR
                     DMPPPSSNFLVTAVFRAGRFEEVPIIDRGARPPLAERTMTPTKLLIGQIIIVFAIVIA
                     GVWAATQWTAAMLGHQPQLGSAWFDFLGVPVYRPWQIFAWWYHFDAYAPHVFDKA"
BASE COUNT          636 a         1282 c         1231 g          659 t
ORIGIN      
        1 ggagtgggaa gccgccgctg ccgggatcga cccccggacc ggggtccagc tcgatggcgc
       61 gggcgcggtg gcgccccgct atgtcgagca ggacggaagg ctcgcctcga tgttcgggaa
      121 tgtatgggaa tggaccggct cggcttatcg tccctatccc ggttatcgac ctgcggaagg
      181 tgccgtcggc gaatataacg gcaagttcat gagcggccag ttcgtgctga agagcgggag
      241 ctgcgcgacg ccgcgcggac acgcccgcgc ctcctatcgc aatttcttct acccgcagca
      301 gcgctggcag ttcaccggcg tgcgccttgc aaaggatctt tgaccatgtt ggaaatggcg
      361 cagtccgtaa cccggccgcg cgaggcgcgg cccgtcgacc ccctgttccg ggccgaagtg
      421 ctgctcggtc tcggtcaggc ccagaagacg attccggcac gctggcttta cgacagacgg
      481 ggttccgagc tgttcgagga catcacccgg cttgaggaat attatccctc gcgcaccgaa
      541 acggcgctgc tgcgccatca cggccgcgac atagcccttc actgcggccc gggacgcgtg
      601 gtcgtcgaat tcggttcggg aagctcggca aagactccgc tccttctgga ggcggtcgac
      661 ccgtccgcct atgtcgccat cgacatttgc ggcgaattcc tgcggcacgc gtgcgacgag
      721 cttgccggca ccttcgatga gctcgagatc tggccggtcg aggccaattt catggccccc
      781 gtggcgctgc cgccccggct tgccgggcaa cgcacgctgg gcttcttccc gggatcgacg
      841 atcgggaaca tgctccccgc cgacgcggtc gatcttctcc ggatgatgcg gcacaccctc
      901 ggcgagcgcg cccagctcct gatcggcatc gaccggacca aggccccgag ccggcttctg
      961 cctgcctatg acgacgcgcg aggcgtcacc gcagcgttca acctcaacct gctcgaccgg
     1021 atcaacgacg agctcggggg cgatattccc gtcgcacgct ttcgtcatga agcgcgctgg
     1081 aacgccgact ggcagcgcat cgaaatgcat ctcgctgcaa ccgggccggt ctcgttcagc
     1141 gtcgccgggc gacgcttcgc gatgcttgcg ggcgagacga tccacaccga aaacagccat
     1201 aaatatgcgc ccgaacaggc aggcctgctg cttcgcgcgg gcggctggtc tcccgtccag
     1261 caatggaccg acgggaacgg cgactttctt ctcctgctca ccgaggcgac gccgtccgat
     1321 ttgtctccct gagcgatcgg catcgcggcg gccatcgcat cgccgggatt tacccgtcca
     1381 gccgcgcggt gcgtcgattt tcgacgcccc gatatgaaag ggccgccggg cttgcgcccg
     1441 gcggccaagt gggtccgggt gggggaggga gtgaacccgg accggggtca gctgctcagg
     1501 ctcgcgatcg cggcgacctg catgctggaa agacccagct cgacgaggcg cgcgccatat
     1561 tcttcgtccc gcgaacggga gcgcgcgccg caaacgaagg cggagaccgc gtcgactaga
     1621 tcggcttcgg cggcaggccc cctcctttcc ttggccggcg cgcggaagat cgaggcgata
     1681 cggcgcagcg ggccgtcttt gcgtgcgtcg gcgcgatcga gcaaatcgcg caccgcatgc
     1741 cattccgaac gactgagggc gatggtttcg gcgggcggca ctttcaatct ccaggttgat
     1801 ctgttgcccg gactatgacg ctgcgcgccc gcgcgctcca ttatacctaa ggctcgaaac
     1861 tgcccggatg ggtggccggc gtccgggagg ggccgcccca agggtgctgc gaggccggtc
     1921 gccagctgcg cgagcgaaga ggccgtctcg cctcgccgag gcgacctatc cccgcggccc
     1981 cgccgatgac atcttcgggc tcgcgatatc gatgcccggt ctcgccggag cccgcttgat
     2041 gtcaccggcc cgccttctcc tcggccagac cgttattgtc ttcggaagac ggccaatccg
     2101 gcggagccgc cgcgattctg aacgctggcg cgggaaggtc gaacccacgg gcctgcaaga
     2161 gcggaatcac acctccagcc gaaataccgg tcctttgccg tccagtcgaa gttcgtggat
     2221 catgtcgatc cgctgtccat cccaatgata acggaaatgc cgcttctgaa ccggccgggc
     2281 gacgacgtcg ggccagaaag cggcgatgca ttccccgccg cgattgcgga ccgacggata
     2341 gacgattccg ctcgcccccg catcgcgatg ctggcgtgcg aaggcttgcg acggcgcgta
     2401 atcgtcgggg gccagcagca aaccgaaatc gccgtccctg atgtcgacca gctcggcgcg
     2461 gactcgcccg accagctcgc gcatatccga aatccatccg ggctcatcgc gactgtcggc
     2521 gaggcggcgc gcttgatgat gcgtcacttc ggcgactgcc gtcgcgaaat cgcgcgcggc
     2581 ataataggcg ccgaagctgc cgtcgtggaa gcgtcccggc cgatcgggag agcaatgggt
     2641 gaaaggggcc atgacatagc tggcgccagg gccggcgacg cggcgcgcgg cgggaacgag
     2701 atcgagattg ccgatcgtct cggcgatccg cgggttggtc cgggcttcgg cggctgccag
     2761 cagctcccaa tcctcgggat ccgccagatc ctcgaaaaga tcgatcggcg gatatttgct
     2821 ttcgatcagg cgaacggcgg ccgtccagcg gaccgatgcc aggtgcgcat cgggcatccg
     2881 ctgatcacca cgccccgcga tagctgtcga gatagcggcg cacgcgcatc agatcggtga
     2941 gctgcccgcc caacatcacg tcgagcgccg agcggtcgcc aaaagcgcgg ttgggcgcgt
     3001 tgatccagcg gtagccgcgc tcggtctcgg tatagagcag ccgcagcgcc ttgtggatac
     3061 cgatgagatt cgacaggcgc gccgccaggt cgaccgtcca gcggccatat tcgccctgtt
     3121 tccagcgctg gtaggtgcgc accgcgatat cgccgagcag gatcgccgcc tgcgcgtcgg
     3181 tgacgcccca atggccgaac aggcggatga cggcgcgctg catcgcctgg acctcctcgg
     3241 ccgagaactc cggcgcgagg ggtggggccg gacgtttgat ggctaccaat tccaaaaccg
     3301 gaactccttc gtcatttggc gttaatatgg atctttcatg ccaattgtca agaaaacgcc
     3361 gatcatgacg gccgccgaag ccggaccggc ccgcacatct cccgcctctc ctgtgagccc
     3421 tgatcgaatg ccgccggcgc gatatttccg cgcggtggcg cttgcgcgcg acatgcctcc
     3481 gccttcctcg aacttcctcg taaccgcagt tttccgggcg ggccgtttcg aagaggttcc
     3541 aatcatcgac cggggcgctc gtccgcccct tgcggagcgg accatgaccc cgaccaaatt
     3601 gctcatcggc cagattatca tcgtgttcgc gatcgtcatc gcgggcgtat gggccgccac
     3661 gcagtggacc gctgcgatgc tcggacatca gccgcagctc ggctccgcat ggttcgattt
     3721 cctcggcgtg cccgtctacc gcccgtggca gatattcgcc tggtggtacc atttcgatgc
     3781 ctatgcgccc catgtcttcg acaaggcc
//
LOCUS       DATMSU010000322         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_109955,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000322 DATMSU010000000
VERSION     DATMSU010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109955"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VN034_08850"
     CDS             complement(<1..422)
                     /locus_tag="VN034_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV60748.1"
                     /translation="MSEDTPVSVTMEKDGEVAVIIVNNPPVNALSWHVRQGLEDNFSA
                     ALGDDSVKAIVLRCAGATFIAGADISEFGKPPRGPDFNAVLNSIEAASKPVVAAIHGT
                     ALGGGLETALVCHYRVAVPSAKLGVPEVKLGLLPGAGGT"
     gene            complement(483..1685)
                     /locus_tag="VN034_08855"
     CDS             complement(483..1685)
                     /locus_tag="VN034_08855"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV60749.1"
                     /translation="MTHGFDPARLDRIPAFLAAKYIDSGRLPHAATLVSRRGEIAHLS
                     CIGEGRPGEALKEDAIFRIASMTKPVTSIAFMMLVEEGKVALSDPLVKFCPEFRDTGV
                     FVAGGGNVPFLTRPPASPIRTVDLLRHTAGLTYGFQERTPVDGAYRKAKIDDFDADYT
                     MDSFIADLAKIPLQFDPGAHWNYSMATDVLGAVIERIEGKPFAQVLQERIFGPLGMVD
                     TGFKVPADQQHRLADAYAFHPKDKKQDFDSGARSRWAKDRSFHSGGGGLASTLHDYHR
                     FCLMLLGGGKLGEVRIISRKTLALMTSNHLPGGGDLTQHSVGIFSEDENAGIGFGLGF
                     AVTLDPARAGIPGSVGDFYWGGMFSTGFFVDPVEEICMVFMTQLMPSSTYPVRREVKT
                     LVHAALDD"
     gene            complement(1699..>2051)
                     /locus_tag="VN034_08860"
     CDS             complement(1699..>2051)
                     /locus_tag="VN034_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60750.1"
                     /translation="GPGAYSASKAGVINLAKVAAQQLSTSGVRVNAVCPGLTETGMTK
                     PTFDYAKAKEVTHKLGQLNPLRRAAQPEELANVALFLASDQASYVNGQAIAVDGGLTS
                     SHPVTRQLLGQTSH"
BASE COUNT          349 a          643 c          706 g          353 t
ORIGIN      
        1 gtgccgcccg cgccggggag cagaccgagt ttgacctcgg gcacgccgag cttcgccgag
       61 ggcactgcga cgcggtaatg gcagacgagg gcggtctcca acccgccgcc gagcgccgtc
      121 ccgtggatcg cggcgacgac gggtttcgat gcggcttcga tgctgttgag cacggcgttg
      181 aaatcggggc cgcgcggcgg cttgccgaat tcgctgatgt cggcgcccgc gatgaaggtc
      241 gcgccggcgc agcgcagcac gatcgccttg acgctgtcgt cgccgagcgc ggcgctgaaa
      301 ttgtcctcaa ggccctgccg gacgtgccag ctcagcgcgt tgacgggtgg gttgttgacg
      361 atgatgacgg cgacttcgcc gtccttttcc atcgtgacgc taacgggtgt gtcttcggac
      421 atgggagact ccttcaatct ggaacccgtt cgcgcggggc gaacggaaag gggtgttgca
      481 tttcagtcgt cgagcgcggc gtggactagc gtcttgacct cgcgccgaac gggataggtg
      541 gatgagggca tgagctgcgt catgaaaacc atgcagatct cttcgaccgg atcgacgaag
      601 aagccggtcg agaacatgcc gccccagtag aagtcgccga ccgagccggg aatgcccgcg
      661 cgcgccgggt cgagcgtgac cgcgaagccg aggccgaagc cgatcccggc attctcatcc
      721 tcgctgaaaa tgccgacgct gtgctgcgtc aggtcgccgc cgccgggcaa atggttcgac
      781 gtcatcagcg ccaatgtctt gcggctgatg atccgcacct cgcccaattt gccgccgccg
      841 agcagcatca ggcagaagcg atgatagtcg tggagcgtcg aggcgagccc gccgccgccc
      901 gaatgaaagc tacgatcctt cgcccagcgg ctgcgcgcgc cgctgtcgaa atcctgcttc
      961 ttgtccttcg gatgaaaggc ataggcgtca gcgagccggt gctgctggtc ggcggggacc
     1021 ttgaaaccgg tatcgaccat gccgagcggg ccgaaaatcc gctcctgcaa cacttgcgca
     1081 aagggcttgc cctcgatgcg ttcaatgacg gcgccgagca catcggtcgc catcgaataa
     1141 ttccagtgcg cgccggggtc gaactgcagc ggaatcttcg cgaggtcggc gatgaagctg
     1201 tccatcgtat aatcggcgtc gaaatcgtcg atctttgcct tgcggtacgc gccgtcgacc
     1261 ggagtgcgct cctgaaagcc gtaggtcagc cccgcggtgt ggcgcagcag gtcgaccgtg
     1321 cggatcggcg acgcgggcgg ccgcgtcaaa aaggggacat tgccgccccc cgcgacgaag
     1381 acgccggtgt cgcgaaactc ggggcagaac ttcactagcg gatcgctgag ggcgaccttg
     1441 ccctcttcga ccagcatcat gaaggcgatg ctggtgaccg gcttggtcat gctcgcgatg
     1501 cggaagatcg catcctcttt cagcgcctcg cccggccggc cctcgccgat gcacgacaga
     1561 tgcgcgatct cgccgcgccg cgagacgagc gtcgcggcgt gcggcaagcg gccggagtcg
     1621 atatatttgg cggcgagaaa ggcggggatg cggtccagcc gcgcggggtc gaatccatgg
     1681 gtcataggag gggacctatc agtgcgaggt ctggccaagc aactggcgcg tcaccgggtg
     1741 cgagctggtc agcccgccgt cgaccgcgat tgcctgcccg ttgacatagc tcgcctgatc
     1801 gctcgcgagg aacagcgcga cattggcgag ctcctcgggc tgcgccgcgc ggcgcaaggg
     1861 gttcaattgg ccgagcttgt gcgtgacctc cttcgccttg gcataatcga aggtcggctt
     1921 ggtcatcccg gtttcggtga ggccggggca gacggcgttg acgcgcacgc cgctcgtcga
     1981 cagctgctgc gccgcgacct tggcgaggtt gatgacgcct gccttcgacg ccgagtaagc
     2041 gcccgggccc g
//
LOCUS       DATMSU010000323         1901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_63041, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000323 DATMSU010000000
VERSION     DATMSU010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63041"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VN034_08865"
     CDS             complement(<1..184)
                     /locus_tag="VN034_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60751.1"
                     /translation="MPGSRVKLPGLRRWWSVALVAPLLLFLLASFVAPVLMMLSRAVV
                     DRDLERVWPDSAVALRK"
     gene            complement(165..1217)
                     /locus_tag="VN034_08870"
     CDS             complement(165..1217)
                     /locus_tag="VN034_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWV60752.1"
                     /translation="MKAWARLLGIAALTASVAACGSSSELRPLTVVGWGGSSQDAHRK
                     AYWTSFSRTTGIPLREDSWRGGVGVIRTKVIGGDASWDIVQVETEDLILGCEEGLFQR
                     LDWAALGGREAFLPAAVHDCGVGAMLWSYVIGYDGDRIKGPGPKSWADFWDVKRFPGK
                     RGMRKTPKYSLEFALMADGVPADQVYPTLRTPEGIDRAFRKLDEIKPNVVWWSSISQV
                     PDLLGSREVVMSVTSPGRLIVANRTEGRNFKLDWHGNIYAVDYWVILANSPRKAEAAQ
                     LLRYMTRPENQMRLASHIPTGLTNVEANRRVDPKLKLDTPSDPANMTDALELDANFWV
                     EYGDQLTQRFNAWVAR"
     gene            complement(1214..>1901)
                     /locus_tag="VN034_08875"
     CDS             complement(1214..>1901)
                     /locus_tag="VN034_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV60753.1"
                     /translation="RQRVALARALVFEPDLVLMDEPLGALDRQLREHLQVEIKRIQRA
                     LDLTIVYVTHDQGEALTMSDRIAVFSAGRIQQVGRPDAIYETPANAFVAGFVGENNML
                     AGTVVTRHDDQCAIRLPGGQIVRAMATGAMKAGDAVVAAVRPEHVETSAAPLACCNAI
                     EARVEELAYHGDHSRIRAALDGGGSLLIRTPATPGVAPGQRIPVGWCTDRCFAFPAED
                     RAALQQREVA"
BASE COUNT          324 a          635 c          624 g          318 t
ORIGIN      
        1 acttccgaag cgcgactgcg ctgtcgggcc agacgcgttc gagatcgcgg tcgaccaccg
       61 cgcgcgacag catcatcagc accggcgcga caaagctcgc gagcaggaac aggagcagcg
      121 gcgcgacgag cgcgacactc caccagcgcc ggagccccgg cagcttaacg cgcgacccag
      181 gcattgaacc gctgggtgag ctggtcgcca tattcgaccc agaaattggc atcgagttcg
      241 agcgcgtcgg tcatgttcgc cgggtcgctc ggcgtgtcga gtttgagttt cggatcgacg
      301 cgccggttcg cctcgacgtt ggtgagaccg gtcgggatat ggctcgccag tcgcatctgg
      361 ttttcaggcc gcgtcatata gcgaagcagc tgcgccgctt cggccttgcg cgggctgttc
      421 gcgaggatca cccaatagtc gaccgcatag atattgccgt gccaatcgag cttgaagttg
      481 cgtccctcgg tgcgattggc gacgatcagc cgcccggggc tggtcaccga catcaccact
      541 tcgcgcgatc caaggaggtc gggaacctgc gagatcgacg accaccagac gacgttcggc
      601 ttgatctcgt cgagcttgcg gaacgcgcgg tcgatcccct cgggcgtgcg cagcgtcgga
      661 tagacctgat cggccggcac gccgtcggcc atcagcgcga attcgagcga atatttgggc
      721 gtcttgcgca tgccccgttt gccgggaaag cgcttgacgt cccagaagtc ggcccaactc
      781 ttcggccccg gtcccttgat acggtcgccg tcatagccga tgacatagga ccacagcatc
      841 gcgccgacgc cgcagtcgtg taccgcggcg ggcaggaacg cctcgcgtcc gcccaaggcc
      901 gcccagtcga gccgctggaa cagcccttcc tcgcagccga ggatcagatc ctcggtctcg
      961 acctgcacga tgtcccagct cgcgtcgccg ccgatcacct tggtgcggat cacgccgacg
     1021 ccgccgcgcc agctatcctc gcggagcgga atgccggtgg tgcgcgaaaa tgaggtccaa
     1081 taagccttgc ggtgcgcatc ctgcgacgat ccgccccagc cgacgacggt gagcggacgg
     1141 agttcggagc tcgatccgca tgccgcaaca ctcgcggtga gcgccgcgat gccgagaagg
     1201 cgtgcccagg ctttcatgcg acttctcttt gttgcaaggc ggcgcgatct tcggcgggaa
     1261 aggcgaaaca gcggtcggtg caccagccga ccggaatccg ctgtccgggc gcgaccccgg
     1321 gcgtggcggg cgtccggatg aggagcgagc cgccgccatc gagcgccgcg cgaatgcggc
     1381 tatgatcgcc gtgataggca agttcttcga cccgcgcttc gatggcgttg cagcaggcaa
     1441 gcggcgcggc actcgtctcg acatgttcgg gtcggacggc tgcaacaacc gcgtcgcccg
     1501 ctttcatcgc gccggtcgcc atcgcgcgaa caatctggcc tccgggcagc cggatggcgc
     1561 attgatcgtc gtgccgcgtc accaccgtac ccgccagcat attattctcg ccgacgaagc
     1621 ccgcgacgaa cgcgttggcg ggcgtttcat agatcgcgtc gggtcggccg acctgctgga
     1681 tgcgccctgc cgaaaacacc gcgatccggt cggacattgt cagcgcttcg ccctgatcgt
     1741 gcgtgacata gacgatggtg aggtccagcg cgcgctggat ccgcttgatc tcgacctgca
     1801 gatgctcgcg aagctggcgg tcgagtgcgc cgagcggttc gtccatcagc acgaggtcgg
     1861 gctcgaacac cagcgcgcgc gccagcgcga cgcgctggcg c
//
LOCUS       DATMSU010000324         8579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_81215, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000324 DATMSU010000000
VERSION     DATMSU010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81215"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="VN034_08880"
     CDS             complement(<1..1841)
                     /locus_tag="VN034_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60754.1"
                     /translation="MTGRIRAALLAGIASTTLVSPATAQEPVAPPNGQVSLAQNVIDD
                     IVVTAQRRSERAQDVPIVISAFSADALQKLNINEPQDLYGNVPSLVVGTQGQGSRDVQ
                     SYSIRGQSTGFLALPSVASYLSEVPLIAAISLSLQGGPGLFVDLANVQVLSGPQGTLF
                     GRNTTGGAVLFTPQRPTDRLEGYIQGSIGNYNLRSVEGALNLPIIEDKLAIRVAGAYY
                     DRRGFTRDLVWDKWRDDQHWYTGRIGLLMRPAESISNYLMVYGSRSDNNGAGYINRGF
                     RLPSVVAPCNDAVRAPNPSSCDVYRRQTEIANQIGPRATRQDVDSYAKIDTWGAINTT
                     DLELNNELTLRNIISFQKLKSDYASDQDGTPLQARQNSQNANFPNFPVPGLTDEFGIA
                     PNGYDNTLTAGGPRDYLKQFTEELQIQGKMLDGNLNFTAGGFYINTEPAGLWYTSAVQ
                     FCAPQNTGNGALCPGSRARTGVTNRSKAIYAQGTFDFGALSDALDSLRVTAGYRYTWD
                     TAKGFSVNFTPLANGTFRCSSTNRVVSSTDACRYDATLKTSAPTWTVGLDYRPIRDLM
                     IYGKITRGYKAGGFNTTAVNEQFQTFDPEVLTSYEVGFKSDWRLGSVP"
     gene            2198..2983
                     /locus_tag="VN034_08885"
     CDS             2198..2983
                     /locus_tag="VN034_08885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60755.1"
                     /translation="MAENKSSIYTRMARKVVVVAGASSGIGRATALRLGAEGARLVVG
                     SPAVERPQIDSLVEEIKSFDGVVVGTAFDATDDASVRALVQLAVDSFGGVDAFHANFA
                     DLRVIMQDSNAVEVSDEILERTLDVNLKGMLRATRAAVPALVARGGGAMIYTSSTASR
                     AGEPERPCYAMAKAGVNALVRHVASHWGKQNVRANAVMPGFVLVPEKPIPDEFARITL
                     EQTRAVRLGRPEDIAAMVALLVSDDGAWVNGQAIAVDGGATLG"
     gene            3019..3921
                     /locus_tag="VN034_08890"
     CDS             3019..3921
                     /locus_tag="VN034_08890"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWV60756.1"
                     /translation="MIEMPQPEAVVTGLSLPECPRWHGRALYFSDITQGRVYRLNNDF
                     STTIVYENAEDFVGGIGFLDDATLIAVLSKQRLLVRAGPVKPAVHARLDSLCRFVLND
                     MAIASNGRAYVSQPGHDIWAGSMSGMPRPTELILADVDGSVAIAADGLMGPNGVAVSS
                     DGGTLYVAESAAMRITCFSIDRATGHLSERRIFAQLPGGAIPDGICLDDTGAVWAACP
                     ISFIDGSAGRGPGVMRMLEGGKATHIVPMAPGRRALACTLGGAGRTILYICTVPDFDG
                     TLPDAAGEGRIERVATDFAGSGTP"
     gene            complement(3966..4778)
                     /locus_tag="VN034_08895"
     CDS             complement(3966..4778)
                     /locus_tag="VN034_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV60757.1"
                     /translation="MDRLKGKVAIVTGAGRIGNIGVAICEAFLREGAKAVIGTDMRTD
                     GAEAIAAQIGEADSRRFRLVQQDVTSATGWEQLVAKVTAEFGGLDILVNNAGIAIHGG
                     IEQSSLDDLRKVMAVNHDALFLGMKYCAPALAEAIKRFPGGGAIVNNCSMASYKPNAQ
                     NVGYHVSKAAARMLTMCGAVEFGSCRIRVNSVHPGLTMTPLIYEALEEYSASGMFNSV
                     EEGLADIANRNPLRTAGQPEDAAAAFVYLASDEARFVTGASIIHDGGLGERY"
     gene            complement(4814..5965)
                     /locus_tag="VN034_08900"
     CDS             complement(4814..5965)
                     /locus_tag="VN034_08900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV60758.1"
                     /translation="MLAIFAESMLSPAGMHLVAPSLPLMQAEFMGVAQADILVPMVVT
                     LPGLMIALCAPFMGTLGDRIGVRPLLIGSTLSYIFLGMLPYWLQSLHLIVASRFLFGV
                     AESALAVCGTPLIAAAFAARRRQAVIGGRIAFIGLASMGLALCGGWAATFGWRTAYLL
                     YAFAVVPLILVLAFVPRSVFIVAVEKQEGRQPRQLWAIYGLNVLYGTGIAAVFVYATF
                     LLKELGIHTPKLAGSLSAASSFTTVAAALIYPFVVRRLDARVLVVASFLLMAAGCGMM
                     AVANGTAHVAAGLVLTAAGMGFFIPTTTEMLLQYSLPHRRGQATGLGMMSIYLGNFMV
                     PFAMTATTRSGFTVGNGYSWLSAAYLAAAVGSVVLARTVFVPRAGRAVF"
     gene            complement(6212..6649)
                     /locus_tag="VN034_08905"
     CDS             complement(6212..6649)
                     /locus_tag="VN034_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV60759.1"
                     /translation="MDRLEQNKQTVACFWAAFSECRIEDVLNLLHDNATWWVAGDLPM
                     VSGTLAKDQCGPMIAGIASGTQDGVHVTPGTMTAEEDRVAMEATSFGITVDGIEYRNT
                     YHMLHVVRDGKILSVREYMDPGRIAAAFPTFTGTGAVNSRIEG"
     gene            complement(6660..8150)
                     /locus_tag="VN034_08910"
     CDS             complement(6660..8150)
                     /locus_tag="VN034_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HWV60760.1"
                     /translation="MTDPVIPLVIRGKIIRDNLKSYTTRGGKQTFHSPDMTKYIDQLV
                     LTDPCDQQDLYDLTLDEIVDYLVELGNRLTLEDNSHLRQALDMSLGTSSFSDEMLRFQ
                     FGLIPQILSRQAIEHQIESSIGAAYLKGWVKESAGGRDYAVRAFGARAVHVIAGNGWV
                     IALQTLIVNAITRSDAIIKIPSNDLYFCLAVVATMIEMAPDHPITKHVSVAYWKGGDA
                     AVEKPLYKPLNLEKIVAWGGFASMESIRNYLGPGLDLVALDPKSSASIIGSEAFTSDE
                     TMAQAAELAARDIGYLNQAGCINARTLYIQTGTDDRGIQRANAFGRAVFEAMQNLPAN
                     LSSAHPSFDPTLREEIEGVRHSGTFRVFGGRSAEGAIIVSQYPESVDFSDRLDCRVAN
                     LVPVDEIDEAIARLTVHTQTIGIFPDPLKAQIRDKCALRGGQRMTSLGTATVGGFGGP
                     QDGIEPVRRMVRWIRDDTAATEGHVGPFGATSMDAGPAVAAPAHVE"
     gene            complement(8193..>8579)
                     /locus_tag="VN034_08915"
     CDS             complement(8193..>8579)
                     /locus_tag="VN034_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60761.1"
                     /translation="RIPHRYNENYGMSEINAVARLCSAGQFHFPPWVFLSVLDPDTGT
                     PLERKGEVVGRLALFDILPRTYWGGCVSGDRVTVQYDGQCDCGRQGPRISPDIARYSS
                     MKDDDKITCAASAGAYERATDLTLSA"
BASE COUNT         1765 a         2539 c         2561 g         1714 t
ORIGIN      
        1 ggcacggaac ccagccgcca atccgacttg aaacccactt cgtagctggt caggacttca
       61 ggatcgaagg tctggaactg ttcgttaaca gcggtcgtgt tgaagccgcc ggctttgtat
      121 ccgcgggtta tttttccata aatcatcaag tcacggatcg gacgataatc taagccgact
      181 gtccaggtcg gagcgctcgt cttgagcgtt gcatcgtaac ggcaggcatc ggtggatgag
      241 accaccctat tagtcgagga acagcggaac gtgccgttgg caagtggggt gaagttaacg
      301 ctgaagccct ttgccgtgtc ccatgtatag cgatagccag cggtcacgcg gaggctgtcg
      361 agcgcatccg agagcgcgcc gaagtcgaaa gtgccctggg cataaattgc ctttgaacgg
      421 ttggtcacgc cggtgcgggc gcggctaccg gggcacagcg cgccattccc ggtgttctga
      481 ggagcgcaga actgcacggc ggacgtatac cacagaccgg ccggctcggt gttgatgtag
      541 aatcctccgg ctgtgaagtt gagattgccg tccagcattt taccttgaat ttgcagttcc
      601 tccgtaaatt gtttgaggta atcgcgaggg ccgccggcgg tcagggtgtt atcgtatcca
      661 ttgggcgcaa tcccgaattc gtccgtcagg ccagggaccg gaaaattcgg gaagttcgca
      721 ttctgactgt tttgacgtgc ttgcagaggc gtcccgtctt gatccgacgc atagtccgac
      781 ttcaacttct gaaagctaat aatattacgc aacgtcagtt cgttgttcag ttccagatca
      841 gtcgtgttga tcgcgcccca tgtgtcgatc ttggcatagc tatcgacatc ctggcgggtg
      901 gcgcgcgggc cgatctgatt ggcgatctcc gtctgacggc gatagacatc gcatgatgag
      961 gggttgggcg cgcgaactgc gtcgttgcac ggcgcgacca cggacggcag ccggaagccg
     1021 cgattgatgt aaccagcgcc attattgtcc gaacgcgatc catagaccat cagatagttg
     1081 cttatcgact ccgctggcct catcaggagg ccgatccggc cagtatacca gtgctggtca
     1141 tcacgccatt tgtcccagac cagatcacgc gtgaagccac gacggtcgta atatgcgcca
     1201 gcgacgcgga tcgcgagctt gtcttcaatg atcggcaagt tgagcgcgcc ttcgacgcta
     1261 cgcaggttgt agttgccaat gctgccttga atataacctt ctaagcgatc cgtcggccgc
     1321 tgcggcgtga acagcactgc gccgcccgtc gtattgcggc cgaacaaggt gccttgcggg
     1381 ccggacagga cttgcacatt ggcaagatcg acaaaaagtc cgggcccgcc ctgcaagctg
     1441 agactgatcg cagcgatcag tggaacttcg ctcaaatagc ttgcaaccga cggcagcgcg
     1501 agaaagcctg tagactgtcc gcggatagag tagctttgca cgtcgcggct tccctgcccc
     1561 tgcgtgccga caacgaggga aggaacattg ccgtagagat cctgcggctc gttgatgttc
     1621 agtttttgca aagcgtcggc tgaaaaggcg ctgatcacaa taggaacgtc ttgggcacgc
     1681 tctgaacggc gttgcgcagt cacgacgata tcgtcgatga cattctgcgc gagagatacc
     1741 tggccattcg gcggagcgac gggttcttgg gcggtcgcgg gcgaaacaag cgtagttgac
     1801 gcgatgccgg cgagcaatgc ggcgcgaatt cgtcctgtca tgaaaaatcc tctccccgat
     1861 gaatgatcac cccttattgt ggagcgccat ttcgattccc tagactgtaa tttctgcgct
     1921 tgcgtaccac gaatttggta attgtcgtgc gccacgcgcc ggcgaggtcg gcctgaatca
     1981 ggtgagacag tcgggatgtc acggagagca ggttagcgga tattcgctgg gccgcgaggc
     2041 attatatgcc tgtctgtctg ctgactgtta aggtgaaaag catgtgctaa ggaatcaaat
     2101 ctcgttagcc gattgatcct attcttacct ttctgcgcca gcactgcttg atgactggac
     2161 tataactagg caaaattgct agcgagagga gaaaaatgtg gctgagaaca aaagtagtat
     2221 atatactcgc atggcaagga aggttgttgt ggtcgcaggg gcgagcagcg gcatcggccg
     2281 ggcgaccgct ctgcgtttgg gggccgaggg cgcacgcctg gttgtcggtt cgcccgctgt
     2341 cgaacggccc cagatcgaca gtttggtaga ggaaataaaa tcgttcgacg gagttgtcgt
     2401 gggaaccgct ttcgacgcca cggacgatgc ttcggttcgc gcgctggtgc aacttgccgt
     2461 tgatagtttc ggcggagtcg atgcctttca tgctaatttc gcagatcttc gggtcattat
     2521 gcaggacagc aatgccgtag aagtttcgga cgaaatcctg gagcgaacat tagacgtgaa
     2581 ccttaaaggc atgctccgcg cgacccgcgc cgcagttccc gcgctggtcg cccgcggcgg
     2641 tggggccatg atctatactt cgtcaaccgc atcgcgcgcg ggtgagccgg aacgaccctg
     2701 ttatgcaatg gctaaggcgg gcgtgaatgc gctggtccgc cacgtcgcct cgcattgggg
     2761 caagcaaaac gtccgcgcca acgccgttat gccagggttc gttctggtac ccgaaaagcc
     2821 gatccccgac gaattcgcca ggataacgct ggagcagaca cgggcagtcc gcctcggtcg
     2881 gccggaggat atcgccgcga tggtcgccct gctcgtatcg gacgacggcg catgggtgaa
     2941 cggccaggca attgccgtcg atggcggcgc gacgctaggc tgacgatcag gtgacttagc
     3001 cttcagagga aatttgacat gattgagatg ccgcagccgg aggcggtagt gaccggcctt
     3061 tcattgcctg agtgcccccg gtggcacggc agagcgctgt atttttccga tatcacgcaa
     3121 gggcgggtct atcgcctgaa caacgatttc agcacgacca tcgtctatga aaatgccgag
     3181 gatttcgtcg gcgggatcgg cttcctggac gacgcaaccc tgatcgcggt tctgtcgaag
     3241 cagcgactgc tggtccgcgc cggtccggtc aagcccgccg tccatgcgcg gctcgattcg
     3301 ctatgtcgtt tcgttcttaa cgacatggcg attgccagta acggccgcgc ttatgtcagc
     3361 caaccaggtc atgacatctg ggctggcagt atgagcggca tgcccaggcc gacagaactc
     3421 atcctggctg atgttgatgg ctcggtggcc atcgcggcag acggtttgat ggggccgaac
     3481 ggcgttgcgg tcagctccga cggcggcact ctgtacgtcg ccgagtctgc agcgatgcgc
     3541 atcacctgct tctccatcga tcgcgccacg ggacacttgt ctgaaagacg aatattcgcg
     3601 caattgccgg gcggggcaat ccccgacggc atctgcctgg acgataccgg agcggtgtgg
     3661 gcggcctgcc cgatctcatt catcgacggg agtgccggcc gtggtcccgg cgtgatgcgc
     3721 atgttggaag ggggaaaggc gacccatatc gttccgatgg cgccgggacg gagggcgctc
     3781 gcatgcacgc ttggcggtgc cgggcggacg atcctctata tctgcaccgt cccggacttc
     3841 gacggcacgc tgcccgatgc cgccggtgaa ggccgtattg agcgagtggc gacagacttt
     3901 gcagggagcg gcacgccatg agaagcccgg agcatttggc gttcgcgccc ggcagcgccg
     3961 tctccctagt aacgctcccc cagtccgcca tcgtggatta tcgacgcgcc ggtgacgaac
     4021 cgcgcttcgt cggatgcgag ataaacgaag gcggccgcag catcttcggg ctggcctgct
     4081 gtgcggagag gattgcggtt cgcgatatcg gcgagcccct cttccacgga attgaacatg
     4141 ccgctggcgc tatattcctc cagagcctcg tagatcagcg gcgtcatcgt cagcccgggg
     4201 tgcaccgaat tgacgcgaat cctgcatgaa ccaaattcga ccgcgccgca catcgtcagc
     4261 atccgcgcag cagccttcga cacatgatag ccgacattct gcgcattagg cttatacgac
     4321 gccatggaac aattgttgac gatggcaccg ccgcccggaa agcgcttgat cgcctcggcg
     4381 agggccggcg cgcaatattt catacccagg aagagcgcat catgattgac ggccatgacc
     4441 ttgcgcagat cgtccagcga gctttgctcg atcccgccgt gaatggcgat gccggcatta
     4501 ttgacgagga tgtccaggcc accaaattcc gccgtcactt tggccaccaa ctgctcccaa
     4561 ccggtcgcgc tggtcacgtc ctgctggacc aagcgaaagc gacggctatc agcctcgccg
     4621 atctgagccg cgatagcttc ggcaccatcg gtgcgcatat cggtgccgat cacggccttc
     4681 gcaccttcgc gcaagaaggc ctcgcagatc gccacgccga tgttgccgat gcgtcccgcg
     4741 cccgtgacga tcgctacctt gccttttagc cggtccatag aaaactctta gatttccgca
     4801 ataggagttt aagtcagaat accgctcggc ccgcgcgcgg aacgaagacg gtgcgcgcca
     4861 gcacgacgct tcctacggcg gcggcaagat aggctgcact aagccacgag tagccattgc
     4921 ctacagtgaa gccgcttcgc gttgtcgcgg tcatggcgaa cggaaccatg aaattgccaa
     4981 ggtagatgct catcatgccc aatccggtag cttgcccccg tcgatgcggc agtgagtatt
     5041 gcaacagcat ttcggtagtc gtagggatga aaaaacccat gcccgccgca gtcagcacca
     5101 aaccggcagc cacgtgagcc gttccgtttg ctacggccat catcccgcag cctgcagcca
     5161 tcagcaagaa ggatgcgacc accagaacgc gcgcgtctag gcgccgcacg acgaacggat
     5221 agatgagcgc cgccgcaacc gtggtgaatg atgacgcagc cgataagctg cccgccagtt
     5281 taggcgtgtg gatccccaac tccttcagca ggaaggtcgc ataaacaaat acagcggcaa
     5341 taccggtgcc gtagaggacg ttcaggccat agatggccca gagttgccgc ggttgccgcc
     5401 cttcttgctt ttcgacagcg acgataaaga cggagcgcgg cacgaaagcc aggacgagga
     5461 tcaacggcac gacagcgaag gcgtaaagca aataggccgt acgccagcca aatgtcgcgg
     5521 cccatccgcc gcacagcgcc aggcccatcg acgccaagcc gatgaaggcg atccgtcccc
     5581 cgattaccgc ctgccgtctg cgagccgcga aggccgcggc gatcagtggc gtgccgcaca
     5641 ctgccagcgc tgattcggcg acgccaaaaa ggaagcgcga tgcgacgatc agatgcagcg
     5701 attggagcca ataaggcagc atgccgagaa agatgtagga gagcgtcgat ccgatcagta
     5761 agggccgcac gccaatccga tcacccagtg tgcccatgaa tggggcgcac agcgcgatca
     5821 tcagacccgg taaggtgaca accatcggca ccaatatgtc ggcttgcgcg acacccatga
     5881 actccgcctg catgagcggc aatgagggcg ctacgagatg catcccggct ggcgacaaca
     5941 tgctttcagc gaagatcgcc agcaccaagc ctaacgatcc ggccgttaag gcagtagtgc
     6001 tgggcgaagc tgaaccagag gcgtcagccg ccgcaggatc tggagcacgc tcctcgccca
     6061 ttgcaaggct ttcatgcctg atcattgaat ctgccacggc ggctctactc ctctcgtttg
     6121 acggcgcggc acttcgttcg ttgctccagc cattctcgca atcagaccca accgctgtcc
     6181 ggtcaggcaa tgtccatctt ggtatttccg cctacccctc aatgcgcgag ttcaccgcgc
     6241 ctgttccggt gaaggtaggg aaggctgcgg cgatcctgcc cgggtccata tattcacgca
     6301 cggacaggat cttgccatcc cgcacgacat gcagcatgtg ataggtgtta cgatattcga
     6361 tgccatcgac ggtgatgcca aaggaggtcg cctccatcgc cacgcggtct tcctcggccg
     6421 tcatcgtgcc cggcgtgacg tgcacgccgt cctgcgtgcc gctggcgatg ccagcgatca
     6481 tcgggccgca ttggtccttg gcaagagtac ccgataccat cggcagatcg cccgcaaccc
     6541 accatgtggc gttgtcatgc agcaggttga gtacgtcctc aatgcggcat tccgagaaag
     6601 ccgcccagaa gcacgcgacc gtctgcttgt tttgttcaag ccgatccatt cagtctctcc
     6661 tattccacat gcgccggagc cgccaccgcg ggtccagcgt ccatgctggt agcgccgaac
     6721 ggcccgacat gcccttcggt agccgcagtg tcgtcgcgaa tccaacggac catccgccgt
     6781 accggctcga tgccgtcctg cgggccgccg aatccgccga ccgtcgccgt gccgagcgag
     6841 gtcattcgct ggccaccgcg cagcgcgcac ttgtcgcgga tctgtgcctt gagcgggtca
     6901 gggaatatgc cgatcgtctg ggtatgaacg gtcaaccgcg cgatcgcttc gtcgatctcg
     6961 tcgacaggca cgaggttcgc gacccggcag tcgagtcggt cgctgaaatc gacgctctcg
     7021 gggtattgcg acacgataat cgcgccttcc gccgatctcc cgccgaacac tcgaaaagtg
     7081 cccgaatgac gaacaccttc gatctcttca cgtagggtcg ggtcaaatga gggatgcgca
     7141 ctgctaaggt tggctggcaa attctgcatc gcttcgaaca cggcgcgccc aaacgcgttg
     7201 gcacgctgga taccccgatc atcggtgccc gtttggatat agagggtccg cgcgttgata
     7261 cagcccgcct ggttcaggta gccaatgtcc cgagccgcca gttccgccgc ctgcgccatc
     7321 gtctcatcgg acgtgaacgc ttcactgccg ataatagacg cactactctt gggatcgagc
     7381 gccacgagat cgaggccagg gcccagatag ttacgaatgc tctccatcga ggcgaagccg
     7441 ccccaagcga cgatcttttc gagattgaga ggcttgtaga gcggcttttc gaccgctgcg
     7501 tccccacctt tccagtaggc gacgctgaca tgcttggtta tcggatgatc cggagccatc
     7561 tctatcatcg tcgccacgac cgccaggcag aaataaaggt cgttggacgg aatcttgatg
     7621 atggcatcgc tgcgcgtgat ggcgttcacg atcagagtct ggagcgctat gacccagccg
     7681 ttgccggcga tcacatggac tgcacgcgcg ccaaaggcac ggacggcata atcgcggcca
     7741 ccagccgatt ccttcaccca tcccttaaga taggcggcgc caatgctgga ctcgatctga
     7801 tgctctatag cctgacggct caatatctgt gggatcaggc cgaactggaa ccgcagcatc
     7861 tcgtcgctga aactcgacgt gccaaggctc atgtccagtg cctgacgcaa atgactgtta
     7921 tcctccagcg tcagtcggtt gcccaattcg acgaggtagt cgacaatttc atcaagggtc
     7981 aggtcgtata aatcctgctg atcgcatgga tcggttagaa ccagctgatc gatatatttg
     8041 gtcatatcgg gcgaatggaa ggtctgcttg ccacctcgcg tcgtatagga cttgagatta
     8101 tctcggatta tcttccctcg aatgacgaga gggataacag gatcggtcat gatgggctcc
     8161 gcacggtcga atcgttggat aaggtctgta cctcaggcgg acagcgtcaa gtcggtggcg
     8221 cgctcataag caccagcgga agcggcacag gtgatcttgt catcgtcctt catgctgctg
     8281 tagcgcgcga tatccgggct gatgcgcggc ccctgcctcc cacagtcgca ttggccgtca
     8341 tactgaactg tgacacgatc gcccgatacg cagccgcccc aatatgtacg cggaaggatg
     8401 tcgaacagcg cgagccgacc gacgacctct ccctttcgct ccagcggtgt gccggtatcg
     8461 ggatcgagca ccgagaggaa tacccatggc gggaagtgga actgcccggc gctgcacagt
     8521 cgagcgaccg cattgatttc cgacatgccg tagttctcat tgtagcgatg cggaatgcg
//
LOCUS       DATMSU010000325         4313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_147345,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000325 DATMSU010000000
VERSION     DATMSU010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147345"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1545
                     /locus_tag="VN034_08920"
     CDS             31..1545
                     /locus_tag="VN034_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011953067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWV60762.1"
                     /translation="MASQALPASSTSGFSRDFAASIVVFLVAMPLCMGIAVASGLPPE
                     KGLMTGIIGGIIVGMIAGSPLQVSGPAAGLAVIVFELVRTQGMEALGPILLIAGAVQL
                     IAGVAKLGGWFRAISPAVVHGMLAGIGVLIVIGQFHVLFDAKPLASGIENIAAMPGRL
                     LGLNASSPGGAELALLIAGITIGVMLAWEKWRPASMRLVPGALLGVVAGTVIALVLNM
                     NVARVEVPDSILAAITPPTAFASWLTPEIIAAAIAVAFIASAETLLSAAAVDKMHDGP
                     RSDFNKELRAQGVGNMLCGGVGALPMTGVIVRSSANVQAGAVSRRSTIMHGVWILAFV
                     ALLPSVMRAMPMATLGAVLVVTGWRLVKLDHARELLKHHGVLPVVIWATTLVLVVAVD
                     LLTGVIVGILFSLVELIPHVRRLRLKVDHSDNEEGTRVSLSGSATFVHLPKLENTLGS
                     IPRDKAVTIDCGQLSGLDHSCAELLRDWVARRKAHGSPTVLLGDDKRLRRLATA"
     gene            complement(1624..1971)
                     /locus_tag="VN034_08925"
     CDS             complement(1624..1971)
                     /locus_tag="VN034_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HWV60763.1"
                     /translation="MGTVTPLPFATRTAPLQTGFERPELMRILDLYGRMVAAGHWRDY
                     AMDFHRDAAIFSAFRRAAERPEYRIEKRPALRSRQGMWALVSEAGAILKRGDELSNVL
                     APVERKLMKLVGD"
     gene            complement(1971..2690)
                     /gene="cysE"
                     /locus_tag="VN034_08930"
     CDS             complement(1971..2690)
                     /gene="cysE"
                     /locus_tag="VN034_08930"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HWV60764.1"
                     /translation="MFNGLVAYLDSIKARDPAPRSRWEILLYPGLLAVGMHRVAHWLF
                     EARLFFLARFINHLSRFLTAIDIHPGATIGQHLFIDHGFGVVIGETAEIGDNVSIYQG
                     VTLGGTDPANGIGGKRHPTLANDVIVGSGAQILGPVTVHARARVGANAVVTKDVPEGA
                     VMVGIPARSTLLDATEYAREFVPYGTPCSETFDPATQKVELLQCQLEQMQKQLAALLE
                     ERELAAVDDEAPRRKGSRNRA"
     gene            complement(2774..3358)
                     /locus_tag="VN034_08935"
     CDS             complement(2774..3358)
                     /locus_tag="VN034_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60765.1"
                     /translation="MLVSVPHARRAALSALWGLAARLTKLLLDAREPLIGQIKLAWWR
                     DMAAMIANDPAALPKGEPLLAELQASWAGQGGLDALVDAAEAMLLAESDAERRGASES
                     FGAHLFALSGGADAGGKRWGLIWGAGVVEAEPEARALLRDARNSETPARRDFGGSRAL
                     LMLDRWAGAIASHDGERNPRGEGLLLLRIGLFGR"
     gene            complement(3402..>4313)
                     /locus_tag="VN034_08940"
     CDS             complement(3402..>4313)
                     /locus_tag="VN034_08940"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HWV60766.1"
                     /translation="FVMGEEVAEYQGAYKVTQGLLEEFGAQRVVDTPITEYGFAGLGA
                     GAAMGGLRPIIEFMTFNFAMQAIDHIINSAAKTNYMSGGQMRCPIVFRGPNGAAARVG
                     AQHSQNFAPWYASVPGLIVIAPYDAADAKGLLKAAIRTEDPVVFLENELLYGRSFDVP
                     EVDDFVLPIGKARIMRQGSDVTIVSYSIGVGLALEAADQLAGEGIDAEVIDLRTIRPL
                     DTATVLASLKKTNHLVIVEEGWPVCSIASELAMVAMEHGFDDLDAPVMRVCNEDVPLP
                     YAHNLEKAALIDTPRVVAAVKAVRNRA"
BASE COUNT          698 a         1459 c         1420 g          736 t
ORIGIN      
        1 gcaccagcgc ccggcgcagg cggcctgacc atggcgagcc aggctctccc cgccagctcg
       61 accagcggtt tttcgcgcga tttcgcggcg tcgatcgtcg tgtttctcgt cgcgatgccg
      121 ctgtgcatgg gcatcgccgt cgcatccggc ctcccgcccg aaaaaggcct gatgacaggc
      181 atcatcggcg gaatcatcgt cggcatgatc gccggttctc ccctccaggt cagcggcccc
      241 gccgccggcc tcgcggtgat cgtcttcgaa ctcgtccgca cgcagggcat ggaggcgctc
      301 ggccccatcc tgctcatcgc gggcgccgtc cagctgatcg cgggcgtggc gaaactcggc
      361 ggctggttcc gcgcgatttc gccggcggtc gtgcacggca tgctcgccgg catcggcgtg
      421 ctgatcgtca tcggccagtt ccacgtcctg ttcgacgcca agcccttggc aagcggcatc
      481 gagaatatcg ccgcgatgcc gggccgcctg ctgggcctca atgcgagcag ccccggcggc
      541 gccgaactgg cgctgctgat cgccggcatc acgatcggcg tcatgctcgc gtgggaaaag
      601 tggcgtccgg cctcgatgcg cctcgtcccc ggcgcgctgc tcggcgtcgt cgcgggcacc
      661 gtgatcgcgc tggtcctgaa catgaatgtg gcgcgcgtcg aagtgcccga ctcgatcctc
      721 gccgcgatca ccccgccgac ggccttcgcg agctggctga cgcccgaaat cattgccgcc
      781 gccatcgcgg tcgcgttcat cgccagtgcc gagactttgc tgtcggcggc ggcggtcgac
      841 aagatgcacg atggtccgcg cagcgatttc aacaaggaac tgcgcgcgca gggcgtcggc
      901 aacatgctgt gtggcggcgt cggcgcgctg ccgatgacgg gcgtcatcgt ccgcagctcg
      961 gcgaacgtcc aggcgggtgc ggtgtcgcgt cgctcgacga tcatgcacgg cgtctggatc
     1021 ctcgccttcg tcgcgctgtt gccgtcggtg atgcgcgcga tgccgatggc gacgctcggc
     1081 gcggtgctcg tcgtgaccgg ctggcggctg gtcaagctcg accatgcccg cgagctgctc
     1141 aagcatcacg gcgtgctgcc cgtggtgatc tgggcgacga cgctcgtact cgtggtcgcg
     1201 gtcgacctgc tcaccggcgt gatcgtcggc atcctcttct cgctcgtcga actcatcccc
     1261 catgtccgcc gcctgcggct caaggtggat cattcggaca atgaagaggg gacgcgcgtc
     1321 agcctgtcgg gctcggcgac cttcgtccat cttcccaagc tggaaaacac gctgggttcg
     1381 atcccgcgcg acaaggcggt cacgatcgac tgcggccaat tgtcggggct cgaccacagc
     1441 tgcgccgaac tgctgcgcga ctgggtcgca cgccgcaagg cgcatggcag cccgacggtg
     1501 ctgctgggtg acgacaaaag gctgcggcgc ctcgcgacag cctgatcaga gctcccctac
     1561 gggaggaacg ggtgtgggtc aggcgaccag agagaggcct gatccacact cgcccgttcg
     1621 gggctaatcc ccgacgagct tcatcagctt gcgttcaacc ggagcgagca cgttagaaag
     1681 ctcgtcgccg cgcttcaaaa tcgccccggc ttcggagaca agggcccaca tgccctgccg
     1741 gctccgcaag gcggggcgtt tttcgatgcg atattcgggg cgctcggcgg cgcggcgaaa
     1801 ggccgaaaag atcgcggcgt cgcggtggaa gtccatcgca tagtcgcgcc aatgccccgc
     1861 cgcgaccatt cggccgtaaa ggtccagaat acgcatgagt tcggggcgct cgaagccggt
     1921 ctgcaacggt gccgtccggg tcgcaaacgg cagaggcgtt accgtcccca tcaggcccgg
     1981 ttccggctcc ctttgcgccg cggcgcttcg tcatcgacgg cggcaagctc gcgttcttcg
     2041 agcagcgccg cgagctgctt ctgcatctgc tcgagctggc attgcagcaa ttcgaccttt
     2101 tgcgtcgcgg ggtcgaacgt ctcgctgcac ggcgtgccat agggcacgaa ttcgcgcgca
     2161 tattcggtcg cgtcgagcag ggtcgagcgc gccggaatgc cgaccatcac cgcgccctcg
     2221 ggcacatcct tcgtcaccac ggcgttggcg ccgacgcgcg cgcgcgcgtg aaccgtgacg
     2281 gggcccagga tctgcgcgcc cgaaccgacg atcacgtcat tggcgagcgt cgggtggcgc
     2341 ttgccgccga tgccgttcgc gggatcggtc ccgccgagcg tcacgccctg atagatggac
     2401 acattgtcgc cgatctcggc ggtctcgccg atcacgacgc cgaagccgtg atcgatgaag
     2461 agatgctgcc cgatcgtcgc acccgggtgg atgtcgatcg cggtcagaaa gcgcgacagg
     2521 tgattgatga agcgcgcgag gaagaacagc cgcgcctcga aaagccaatg cgcgacgcgg
     2581 tgcatgccga ccgcgagcag ccccggatag agcaatattt cccagcgcga ccgcggtgcg
     2641 ggatcgcgtg ctttgatcga atcgagatag gcgaccagcc cgttgaacat gcaatgtccc
     2701 ctgcgaccct atttgttgcg gccaagatag agggtcgggg acatcatttc cacccccggc
     2761 gcgtttttcg tgatcagcgt ccgaaaagac cgatacgcag gaggagaagt ccttcgccgc
     2821 gcggattccg ttcgccgtca tggctggcga tcgctcctgc ccagcgatcg agcatcagaa
     2881 gcgcgcggct tccgccgaaa tcgcggcgtg cgggcgtttc ggaatttcgc gcatcgcgca
     2941 gcaacgccct tgcttcgggt tcggcttcga cgacgccggc gccccagatg agaccccagc
     3001 gcttgccgcc ggcgtctgcg ccgccggaca gcgcaaaaag atgcgcacca aagctttcgg
     3061 acgcgccgcg ccgttcggca tcgctttcgg cgagcagcat cgcttccgcc gcatcgacga
     3121 gcgcgtcgag cccgccctgc cccgcccagc ttgcctgaag ctcggcgagc aagggttcgc
     3181 ccttgggaag cgccgcgggg tcgttcgcga tcatcgccgc catgtcgcgc caccaggcga
     3241 gcttgatctg cccgatcagc ggctcgcgcg catcgagcaa cagcttggtc agccgcgcgg
     3301 ccaatcccca aagcgccgac aacgccgcgc gccgcgcgtg cggtacggag accagcacgc
     3361 gcggatcgcg catatcggga tagaaagccg cctccggatc gtcaggcgcg attgcggacc
     3421 gccttcacgg ctgccacgac gcgcggcgta tcgatcagcg cggccttttc gagattgtgc
     3481 gcatagggca gcggcacatc ctcgttgcac acgcgcatca cgggcgcatc gaggtcgtcg
     3541 aagccatgct ccattgcgac catcgcgagt tcgctggcga tcgaacagac cggccagcct
     3601 tcctcgacga tgacaagatg gttcgtcttc ttgaggctgg cgagcacggt cgcggtgtcg
     3661 agcggacgga tcgtgcggag atcaatgacc tcggcgtcga tcccctcgcc cgcgagctgg
     3721 tcggcggctt cgagcgcgag cccgacgccg atcgaatagc tgacgatggt gacgtcgctg
     3781 ccctgacgca tgatccgcgc cttgccgatc ggcaggacga aatcgtcgac ctcaggcacg
     3841 tcgaagctgc ggccatagag cagttcgttt tcgaggaaga cgacggggtc ttcggtgcgg
     3901 atcgcggcct tcagcagccc cttggcatcg gcggcgtcat aaggcgcgat cacgatcagg
     3961 ccggggacgc tggcatacca gggcgcgaaa ttctggctgt gctgcgcgcc gacgcgcgcc
     4021 gcggcgccgt tcgggccgcg gaacacgatc gggcaacgca tctggccgcc cgacatatag
     4081 ttggtcttcg ctgccgagtt gatgatgtgg tcgatcgcct gcatcgcgaa gttgaaggtc
     4141 ataaactcga tgatcggacg cagcccgccc atcgcggcgc ccgcaccgag accggcgaag
     4201 ccatattcgg tgatcggggt gtcgacgacg cgctgcgcgc cgaactcctc gagcagcccc
     4261 tgcgtgacct tgtaagcacc ctgatactcg gcgacttcct cgcccatcac gaa
//
LOCUS       DATMSU010000326         6059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_110010,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000326 DATMSU010000000
VERSION     DATMSU010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6059
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110010"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VN034_08945"
     CDS             complement(<1..242)
                     /locus_tag="VN034_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWV60767.1"
                     /translation="MIDRRRFMGAAAATGLSGFVPSALNAQEGAADRALEALLQRHSE
                     AYLRRSPEEATANNFDTGAHAVLRSRLDDRSLAARA"
     gene            complement(327..2615)
                     /locus_tag="VN034_08950"
     CDS             complement(327..2615)
                     /locus_tag="VN034_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60768.1"
                     /translation="MYCINQEKRGTGRFAAGVAAAAIIGGLVAAPAHAQEPGASESAG
                     AGEIVVTAQRREQSLQDVPIAITALSAETIKNQNIQSVDDYFAMTPNVSFQSNGSRDR
                     KDLAIRGISNQLNPYADVRQSSYAFYVDEFNVAAGTSNPQIVDLERIEVLRGPQGTYF
                     GRNAVGGAINVITKKPVDAFEGELELGYSSFDTKRAQGVINVPVVSGLLAVRASGQIE
                     KSDGYIKNINPIGGGNDSEYYTGRIQARFTPLDNLTWDFTYNYSDEEVGMRNGVPTGF
                     VTRTWASVYYGAAAGTIADEDGVGFFPDNKTRVNFNRDQRVGSKFEYFSSRAVWDFGP
                     VALTAVGGHLVADIFNYGDVDGGSPDFFYEDLQLRRKSTSGELRLQSTGTQFLDWSLG
                     ASLGKDSGVSEQSTYHGADSPLCPAASAGDCEGLEVTGADGVSSSRYFAVFAQGTLNF
                     SEKFSATAGLRYSWEKVTNISQTRSNTVVTGNNDRVADFEDISPKVTLSYKPNSDWMI
                     FATASRGFKSGGTQTTNSAALTNEFKPETLWNYELGTKFDLFDKRLRVDITGFYMDWK
                     DVQQTIRFQFIDPDSGLLRGVSGIANAASAESYGVEASFDARITDHWTLGGNVGFNKS
                     KYKDYPNALIDGVVIDISGEQLTNAPEWTLGAQTQYTLPIGDRFEAFVRAEWNYRDAM
                     ISNPYYYLYKDVVGEPFESPSYHNVNLRLGVEDDRVRAAVFVENLFDADYYANAYEKA
                     FYSGVQVEPSYRVFGVSIGYKF"
     gene            complement(2694..3179)
                     /locus_tag="VN034_08955"
     CDS             complement(2694..3179)
                     /locus_tag="VN034_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60769.1"
                     /translation="MKKTLILASALAVLNFAAPAAAQDKPATDVLSKMFIWWNEAFRT
                     PGAYTPENFSKFFTPDATLVLEGRTVIDGVDQWATHFQRIQSGGGDVEIVVPFKDVFE
                     ADGRVYTYHVIRSRRDGKTGCSLAAGHAELEGGKIASIVLVRHTLEAGKDPLDPQCWT
                     K"
     gene            complement(3571..4830)
                     /locus_tag="VN034_08960"
     CDS             complement(3571..4830)
                     /locus_tag="VN034_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV60770.1"
                     /translation="MKYRLLSAAVPLAIVVGAPAAAQPVALVGGNVVDLAGKAPLRDA
                     VVLIDGERIQAIGPRASTPVPPGATVIPMDGKWLIPGLMNMHVHLALNLPGASRIYNE
                     TPDSMALRTLDNAQKSLWSGVTTIRLTGSDAGVDFTVKRAIDSGVFDGPRIHTAGSSI
                     VATGGHGKTEVNGAAEFSRAVREQVKAGATWIKLGISGGISDTHGDIGASPFTDDELT
                     TAIEVAHRNGVKVTGHTGSPIASEAAIDAGIDCFEHGYFLTPPVLAKMKKKGVWYVPT
                     IVVSQSGAKAFYRKIGSPDWYLDRVDSVGKAHIQSLRDAISAGVNIALGTDQYPWEPN
                     EGTTATVREAEIYVDAGMTHAQALRAATIEPARMLGVDNEVGLLKAGYYADIIAVDAD
                     PTQDIRALRTIGFVMKGGKLIRNDAAR"
     gene            complement(4851..>6059)
                     /locus_tag="VN034_08965"
     CDS             complement(4851..>6059)
                     /locus_tag="VN034_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV60771.1"
                     /translation="TALVGGTVVGIDGGAPISNATILVQGERILRVGPAASVDVPAGA
                     KVVPMNGKWLIPGLMNMHVHFGLKLPGAAGAELINEKDGEEVLRMADNARRTLLAGVT
                     TVRLTAEDRGLDFMLKRAITKGQAVGPRIETAGELIVPTGGHGSLEADGPAEFSKAAR
                     TQIKGGASWIKIAISGGISDTHGSISAAPMTDAELSTIIEVAHRNGVKVTAHNGSSEA
                     ALQALKFGIDCFEHGYHLNDEVLAKMKAQGTWLVPTIVVSQPGAYEFYRKIGSPEWYL
                     DRVASTGKDHWAVLQKAIKLGVNVALGTDQFPFEPNGGTTATVAEAELYQKAGMTPLQ
                     ALQAATIKPAAMLGIDKDVGRIVAGQYADIVAVTADPAKDIAALRTLDFVMKGGVIYR
                     NDAMPETIAH"
BASE COUNT         1057 a         1927 c         1871 g         1204 t
ORIGIN      
        1 ctggcgcgcg ccgccagcga ccggtcatcg agccggctgc ggagcaccgc atgcgcgccc
       61 gtgtcgaaat tgttcgccgt tgcttcctcg ggcgagcgcc gcagataggc ttcgctgtgc
      121 cgctgcagca acgcctccag cgcgcgatcc gcagcgcctt cctgcgcgtt tagcgcagac
      181 ggcacgaaac ctgacaagcc ggtcgccgcc gcggctccca taaagcgtcg acggtcgatc
      241 atgacgttac tccggtgtaa tattgtcgtt ggtcggtcgg taagcagacg gggggcggcc
      301 gcgaccagga ccgccccccg ttcgcgtcag aacttatagc cgatgctgac accgaagaca
      361 cgataggacg gttcgacctg tacgccgctg taaaacgcct tttcataggc gttggcataa
      421 taatcggcgt cgaacagatt ctcgacgaac accgctgcgc gcacgcgatc atcctcgacg
      481 cccagacgca ggttcacatt atgatagctt ggactttcaa agggttcgcc caccacatcc
      541 ttgtatagat aataataggg gttggagatc atcgcatcgc gataattcca ttcggcgcgc
      601 acgaaggctt cgaagcggtc accgatcggc agcgtatatt gggtctgcgc accgagcgtc
      661 cattccggcg cgttggtcag ttgctcgccg ctgatgtcga tgaccacgcc gtcgatgagc
      721 gcgttcggat aatccttgta cttcgatttg ttaaagccga cgttgccgcc gagcgtccaa
      781 tggtcggtaa tccgcgcgtc gaagctcgcc tcgacgccat agctttcggc cgacgccgcg
      841 ttggcgatgc cgctgacccc gcgcagaagc ccgctgtccg ggtcgatgaa ctggaagcgg
      901 atcgtctgct ggacatcctt ccaatccatg tagaagccgg tgatgtcgac gcgcaggcgc
      961 ttgtcgaaca gatcgaactt cgtcccgagt tcatagttcc agagcgtttc gggcttgaat
     1021 tcgtttgtca gcgccgcgct atttgtcgtc tgcgtcccgc ccgacttgaa gccgcgcgac
     1081 gcggtggcga agatcatcca gtcgctattg ggtttatagc tcaacgtgac cttgggcgag
     1141 atatcctcga aatcggcgac gcgatcattg ttgccggtga ccacggtatt cgaccgggtc
     1201 tgcgagatat tcgtcacctt ctcccacgaa tagcgaagcc ccgcggtcgc cgagaatttc
     1261 tcgctgaaat tcagcgtgcc ctgggcaaag acggcgaaat agcggcttga gctgacaccg
     1321 tcggcgccgg tgacctcaag cccttcgcag tcgcccgcgc tggccgccgg acaaagaggg
     1381 ctgtcggcgc cgtgataggt cgactgctcg ctgacgccgc tatccttgcc gagcgaggcg
     1441 ccgagactcc agtcgaggaa ctgggtccct gtcgattgca ggcgaagttc gccgctggtc
     1501 gatttgcgtc gcagttgcag gtcttcgtaa aagaaatcgg ggctgccgcc atcgacatca
     1561 ccatagttga agatgtcggc gaccagatgc ccgccgacgg ccgtcagcgc gaccggaccg
     1621 aaatcccaga ccgcgcgcga gctgaaatat tcgaacttgc tgccgacgcg ctggtcgcgg
     1681 ttgaagttca cacgggtctt gttgtccggg aaaaagccca cgccgtcttc gtcggcgatc
     1741 gttccggcgg cggcgccata atagaccgaa gcccaggttc gcgtgacgaa gccggtcggt
     1801 acgccgttgc gcatgcccac ttcttcatcc gaataattat aggtgaaatc ccacgtcaga
     1861 ttgtcgagcg gcgtgaagcg cgcctggatg cggccggtat aatattcgct gtcgttgccg
     1921 ccgccgatag ggttgatatt cttgatatag ccgtcggact tttcgatctg gcccgacgcg
     1981 cggaccgcaa gcaatccgct gacgaccggg acattgatca cgccctgcgc gcgtttcgta
     2041 tcgaaactgc tgtagcccag ctcaagctcg ccctcgaacg catcgacggg cttcttcgtg
     2101 atcacgttga tcgcgccgcc gacggcgttg cggccgaaat aggtgccctg cggaccgcgc
     2161 agcacctcga tgcgctcgag gtcgacgatc tggggattgc tcgtcccggc ggcaacgttg
     2221 aattcgtcga catagaaggc atagctcgac tgccggacgt cggcataggg gttgagctgg
     2281 ttcgaaatgc cgcggatcgc aagatccttg cggtcgcgcg acccgttcga ctggaaactg
     2341 acgttcggcg tcatggcgaa ataatcgtcg acgctctgga tattctggtt cttgatcgtc
     2401 tcggcgctga gcgcggtaat cgcgatcgga acatcttgca ggctctgttc gcggcgctgc
     2461 gcggtgacga cgatttcgcc cgcacccgcg ctttccgaag cgcccggctc ctgggcatgg
     2521 gcgggggcgg caaccaaacc tccgatgatc gccgcagccg cgacgcccgc ggcaaagcgg
     2581 ccagtgccgc gcttttcctg attgatgcag tacatgtcaa aaccccctcg ttggccggcg
     2641 atcctgtccg ccggtccctt ggtccaatcc ttttcacggt cgccgtcagg gcgtcatttg
     2701 gtccagcatt gcgggtcaag cggatccttg cccgcctcga gcgtgtggcg gacgaggacg
     2761 atcgacgcga tcttgccgcc ttcgagctcg gcatggccgg ccgcgagcga acagccggtc
     2821 ttgccgtcgc gccgcgaacg gatgacgtga taggtgtaga cgcggccgtc ggcttcgaag
     2881 acgtccttga acggcacgac gatctcgacg tcgccgccgc ccgactggat gcgctggaaa
     2941 tgggtcgccc attggtcgac gccgtcgatc accgtgcgtc cttcgagcac cagcgtcgca
     3001 tcgggcgtga agaatttcga gaaattttcc ggggtatagg cgccgggcgt cctgaacgcc
     3061 tcgttccacc agatgaacat cttcgaaaga acgtcggtcg caggcttgtc ctgggcggcc
     3121 gccggtgccg cgaagttgag gaccgcaagc gcggatgcga gtatcagcgt ctttttcatt
     3181 tggaactccc tgtaactata ttgctttgtt ggcggcctcg tggccgcggt gtttcagcga
     3241 gtgtccgtta gttgcaccgt tgtttcaaca acattacaaa ttcgctttcg ttgcgttgaa
     3301 cgcaaacaac gagcaacccc attctcgtac gcagcgaccg cgctccaccc gatcccggcc
     3361 gtgccaaatt cggaacgata atttttcata ttattatcag ctacttgaat acgacccatc
     3421 ggaagggatt gcgcgacgtg aaggtgcatg gtcccgatcc gggttattgc gcggagaccg
     3481 gcgcgccaga ggtcatcgac gacgggccga ggcgccatcc gtcgatggcg cctgcagcaa
     3541 tgtaacaaaa atcacgcgat tcgttgtcta ttatcgcgcc gcgtcgttgc ggatcagctt
     3601 gccgcccttc atcacgaacc cgatcgtgcg cagcgcgcgg atgtcctgcg tcggatcggc
     3661 gtccaccgcg atgatgtcgg cataatagcc tgccttcagc agccccacct cgttgtcgac
     3721 gccgagcatc cgcgccggtt cgatcgtcgc ggcgcggagc gcctgcgcgt gggtcatccc
     3781 cgcatcgaca tagatttcgg cttcgcggac ggtcgccgtc gtgccctcat tgggctccca
     3841 gggatattgg tcggtgccga gtgcgatgtt gacccccgca gaaatcgcgt cgcgcagcga
     3901 ctggatatgc gcctttccga ccgaatcgac gcgatcgaga taccagtcgg gcgaaccgat
     3961 cttcctgtag aaagccttgg cgccgctctg gctgacgacg atcgtcggta cgtaccagac
     4021 gcccttcttc ttcatcttcg cgaggacggg gggcgtcagg aaatagccat gttcgaaaca
     4081 gtcgattccg gcatcgatcg cggcctcgga cgcgataggc gagcccgtat ggccggtcac
     4141 cttcacgccg ttgcggtgcg ccacctcgat cgcggtcgtc agttcgtcat cggtaaaggg
     4201 cgaggcgccg atgtcgccgt gcgtatccga aattccgccc gaaatgccga gcttgatcca
     4261 ggtcgcgccg gccttcacct gttcgcgcac cgcgcgcgag aattcggcgg caccattgac
     4321 ctcggtcttg ccgtgcccgc cggtcgcgac gatcgacgag cccgcggtgt gaatacgcgg
     4381 cccatcgaac acaccgctgt cgatcgcgcg cttgacggtg aaatcgaccc cggcatcgga
     4441 tcccgtcagc cggatcgtcg tcacccccga ccacagcgac ttttgcgcgt tgtcgagcgt
     4501 gcggagcgcc atcgagtcag gggtttcgtt atagatgcgc gacgcccccg gcaggttgag
     4561 tgcgagatgg acgtgcatgt tcatcaggcc ggggatcagc catttgccgt ccatcggaat
     4621 gaccgtcgcc cccggcggga ccggcgtcga ggcacgcggg ccaattgcct ggatacgttc
     4681 gccgtcgatc agcaccacgg catcgcgcag cggcgccttg ccggcaaggt cgacgacatt
     4741 gccgcccacg agcgcgacgg gttgcgccgc cgcgggtgcc ccgacgacga tggcgagcgg
     4801 gacggcggcg gagagcaggc gatatttcat ggagaccccc tgttatcgat tcagtgcgcg
     4861 atcgtttcgg gcatcgcatc gttgcgatag atgacgccgc ccttcatgac gaaatcgagc
     4921 gtgcgcaacg ccgcgatatc cttggccgga tcggcggtga cggcgacgat gtcggcatat
     4981 tggccggcga cgatccgccc gacgtccttg tcgatcccca gcatcgctgc gggtttgatc
     5041 gtcgccgcct gcagggcctg cagcggcgtc atgccggctt tctgatacag ctcggcctcg
     5101 gcgacggtcg cggtcgttcc gccgttgggt tcgaacggaa actgatcggt gccgagcgcg
     5161 acattgaccc cgagcttgat cgccttctgc aggaccgccc aatggtcctt cccggtcgag
     5221 gcgacgcggt cgagatacca ttcgggcgaa ccgatcttgc gatagaattc ataggcgccc
     5281 ggctggctga cgacgatcgt cggcaccagc caggtgccct gcgccttcat cttcgcgagc
     5341 acctcgtcat tgagatgata gccgtgctcg aaacagtcga tcccgaactt cagcgcctgg
     5401 agcgcagctt cggacgaccc attatgcgcg gtcaccttca ccccgttgcg gtgtgcgacc
     5461 tcgatgatcg tcgacagttc ggcatcggtc atcggcgccg ccgaaatgct gccgtgcgtg
     5521 tccgaaatgc cgcccgagat cgcgatcttg atccagctgg cgccgccctt gatctgcgtg
     5581 cgcgccgcct tgctgaactc ggcggggccg tcggcctcga gcgacccgtg accgccggtc
     5641 ggaacgatca gttcgcccgc agtctcgata cgcggcccga ccgcctgacc tttcgtgatc
     5701 gcgcgcttga gcatgaagtc gaggccgcgg tcctcggcgg tcagccggac ggtcgttact
     5761 ccggcgagca gcgtccggcg cgcgttgtcc gccatgcgaa ggacttcctc gccgtccttc
     5821 tcgttgatca gttcggcgcc cgcggcgccg ggaagcttca gcccgaaatg gacgtgcatg
     5881 ttcatcaggc cggggatcag ccacttgccg ttcatcggca ccaccttcgc tccggcgggg
     5941 acgtcgaccg acgccgcagg gccgaccctc aggatgcgct cgccctgcac caatatggtg
     6001 gcattcgaaa tcggcgcgcc gccgtcgatg ccgacgaccg tgccgccgac cagcgcggt
//
LOCUS       DATMSU010000327         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_176225,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000327 DATMSU010000000
VERSION     DATMSU010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176225"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..832)
                     /locus_tag="VN034_08970"
     CDS             complement(80..832)
                     /locus_tag="VN034_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HWV60772.1"
                     /translation="MRNWTMAILPLALVTGCNGTAPKDEQPAERGGPPASVGPITTQS
                     FDLTGFTGVEVTGPDDVTVRRGDSFSIVARGRQETLDRLEIKIDGSQLSIGRKRDGFS
                     FSNRDDDDLDIAVTMPRLDKLRLTGSGSIDADSVDGDAVEAAVTGSGDLKIAKLTGKD
                     AEIEVSGSGDIEIGGGNIASGDISVTGSGDVDAEALVARDLDISITGSGNVETQATGK
                     AGIRILGSGDVTLTGGAACTTRKLGSGTATCN"
     gene            953..1429
                     /locus_tag="VN034_08975"
     CDS             953..1429
                     /locus_tag="VN034_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HWV60773.1"
                     /translation="MSVAFRRESDDEHKEPAFELPIPVGPNLVTPRGLRLLGEEVVRL
                     EAAIAAETDEDARKKLQRRLRYFHTRQATAEVQAPPAGGSTGIGSRVTYRLNKAEKTI
                     TIVGGDEADPALGTIAFFAPLARALMGAEAGESVEYQDREDAIAILSVETDPETQA"
     gene            1426..1605
                     /locus_tag="VN034_08980"
     CDS             1426..1605
                     /locus_tag="VN034_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008065066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60774.1"
                     /translation="MKDKFERHKQPWTAAEIDKLHTLAKKNMALKAIAKALTRSEESV
                     KVRAKADGLSIAKLR"
     gene            1817..>2617
                     /locus_tag="VN034_08985"
     CDS             1817..>2617
                     /locus_tag="VN034_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HWV60775.1"
                     /translation="MTAPDYDAIVLGAGAAGLMCAAVAGQRGRRVLLLDHADQVGKKI
                     LISGGGRCNFTNIHTAPDRFLSANPHFAKSALARYTAADFIDLVNAYGIAHHEKTLGQ
                     LFCDGSAKQIVAMLLDECAKGGVDVRCGQPVREVTHGDGAFRVRFGDQPFTAVNLVIA
                     TGGPSIPKMGATGFAYDLARQFGLKVVEPRPALVPLTLGGDDVLFRDLSGVATPVEAR
                     AGKAAFREAALFTHKGLSGPAILQVSSYWRHGEPVTIDFLPDAAPDWLL"
BASE COUNT          431 a          905 c          858 g          423 t
ORIGIN      
        1 atcgcgcgcg cggcgcatcg caagacgtgg atcatgcccg ccgtcttagc gctgcgccgc
       61 cgccgccgcc agtccggcgt cagttgcagg tcgcggtgcc cgaacccagc ttgcgcgtcg
      121 tgcaagccgc gccaccggtc agcgtgacgt cgcccgaacc gaggatgcgg atgccggcct
      181 tgccggtcgc ctgcgtctcg acattgcccg acccggtgat cgatatatcg aggtcgcgcg
      241 cgacgagcgc ttcggcatcg acgtcgcccg atccggtgac gctgatgtcg cccgacgcga
      301 tgttgccgcc gccgatctcg atatcgcccg atcccgaaac ctcgatctcg gcatccttgc
      361 ccgtcagctt cgcgatcttg aggtcgcccg atcccgtcac cgcggcttcg accgcgtcgc
      421 cgtcgacgct gtcggcgtcg atcgaccccg atccggtcag gcggagcttg tcgagccggg
      481 gcatggtgac ggcgatatcg agatcgtcgt cgtcgcggtt cgaaaagctg aagccgtcgc
      541 gcttgcggcc gatcgacaat tgcgagccat cgatcttgat ctcgagccgg tcgagcgttt
      601 cctggcgccc gcgtgcgacg atcgagaagc tgtcgccccg acgcacggtg acgtcatcgg
      661 ggccggtaac ctcgacgccg gtaaagccgg tcagatcgaa gctctgcgtg gtgatcgggc
      721 cgacgctggc gggcggaccg cctcgctctg ccggctgctc gtccttcggt gccgtgccgt
      781 tgcagcccgt cacgagcgcc agcggcagga tcgccatcgt ccagttacgc atcgaaaatc
      841 tcctccgtgt attgcttgcg taacacacag acgcgcggcc tgtccagtgc aagccgccat
      901 cttgtcgcca ccttcgtggg ccacaggcgc gcatccttca ggaaaagaga cgatgagcgt
      961 agcctttcgc cgcgagagcg acgacgaaca taaagaaccc gcgttcgaac tcccgattcc
     1021 ggtcgggccg aacctcgtca ccccgcgcgg gctccgcctg ctcggcgagg aggtcgtgcg
     1081 tctcgaagcc gcgatcgccg ccgagaccga cgaggacgcg cgcaagaaac tgcagcgccg
     1141 cctgcgctat ttccataccc gccaagcgac cgccgaggtg caggcgccgc ccgcgggcgg
     1201 cagcaccggc atcggcagcc gcgttaccta ccggctgaac aaggccgaaa agacgattac
     1261 catcgtcggc ggggacgagg ccgacccggc gctgggcacc atcgcctttt tcgccccgct
     1321 cgcccgcgcg ctgatggggg cggaggcggg cgagagcgtc gaatatcagg atcgcgaaga
     1381 tgcgatcgct atcctttccg tcgaaaccga tccggagacg caggcatgaa agacaagttc
     1441 gagcgtcaca aacagccgtg gaccgcggcc gagatcgaca agctccacac gctcgccaaa
     1501 aagaacatgg cgctgaaagc gatcgccaag gcgctgacgc gcagcgagga atcggtgaag
     1561 gtccgtgcca aggccgacgg cctgtcgatc gcaaagctgc gctgatttct ggcgtgacgg
     1621 ctcggcacag cctatccctc ccccttgatg ggggaggcag cgagacttgg cgacgcagtc
     1681 gcctagtcgc agcggtgggg gtgtgccctc gtcctgacgc gatgacgcaa ggccgcaccg
     1741 tcacccccat ccaactccgc ctagcacctg cggcgcaagg ctgcgtatcc ttcccccatc
     1801 aagggggaag gggcaggtga cagcccccga ttatgacgcc atcgtgctgg gtgccggcgc
     1861 ggccgggctg atgtgcgccg cggtcgcggg gcaaaggggg cggcgcgtcc tgctgctcga
     1921 ccatgccgat caggtgggga agaaaattct tatctcgggc ggtggacgct gcaatttcac
     1981 caacatccac accgcgcccg accgcttcct ctcggcgaac ccgcatttcg ccaagtcggc
     2041 gctcgcgcgc tataccgccg ccgatttcat cgatctggtg aacgcttacg ggatcgcgca
     2101 tcacgaaaag acgctcggcc agctcttctg cgacggctcg gcgaagcaga tcgtcgcgat
     2161 gctgctggac gaatgcgcga agggcggggt cgatgtccgc tgcggccagc cggtgcgcga
     2221 ggtgacgcac ggcgacggcg ccttccgcgt gcgtttcggc gaccagccct tcaccgccgt
     2281 caacctcgtc atcgcgaccg gcgggccgtc gattccgaaa atgggcgcga ccggcttcgc
     2341 ctatgacctc gcgcgccagt tcgggctgaa ggtggtcgag ccgcgccccg cgctcgtccc
     2401 gctgacgctc ggcggcgacg atgtgctgtt ccgcgacctg tcgggggtcg cgaccccggt
     2461 cgaggcgcgc gcgggcaagg ccgccttccg cgaagccgcg ctcttcacgc acaagggcct
     2521 gtcggggccc gcgatccttc aggtcagcag ctattggcgg cacggcgagc cggtgacgat
     2581 cgacttcctt cccgatgcgg cgccggactg gctgctc
//
LOCUS       DATMSU010000328         4261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_157563,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000328 DATMSU010000000
VERSION     DATMSU010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157563"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VN034_08990"
     CDS             <1..448
                     /locus_tag="VN034_08990"
                     /inference="COORDINATES: protein motif:HMM:NF037461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HWV60776.1"
                     /translation="VESAPIASPGGALAARSVDVRAQEDGKSFTWTGPATLELTGPYA
                     DLSRQLNNSFALRVDWRIDAAGGAPVSLALGGKAFDISAAVKAAAQGQVSTIKIPLRC
                     FADAGANLRQVDNAVTVRADKGFAATLLNTNVEAVGENLPCPPAAK"
     gene            495..1802
                     /locus_tag="VN034_08995"
     CDS             495..1802
                     /locus_tag="VN034_08995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HWV60777.1"
                     /translation="MALAPNVATSAEPNAAGQPGRSGRSTRVVLILAFAVFFLLGGVT
                     NINDLLVGKFKPMFHLTHAEANLVQMAFFIAYGAFSIPAGIIMSKLGYIRTFVLGFII
                     VAAAAFLFIPASAAASYPGFLAALFCIGAGITVLQVAMNPVITTLGPVETSHSRLTFA
                     QAFNSIGVFLMVYGGAAFLLGDEQPIDAETSTEAQIQAYRVAEGASIGTAYMWLGVLM
                     LVIAAVFWMYRSAIDHAKTDDVKLEGTWGLLTHNRRVQFGALCIFVYVGAEVAIGSNL
                     MAYLAEPSVMGLGLQAAGKLVAMYWLGALVGRLLGGFILRLAKPGRVLTTFAAGAISL
                     IILSAVTTGALSGWALILVGFCNSLMFPTIFSLATEGLGERTPQGSGIMCTAIIGGAI
                     VPFLFGTVADLSGDIRIALAVPLICYAIIASFGLWAASRPTTA"
     gene            complement(1878..3014)
                     /locus_tag="VN034_09000"
     CDS             complement(1878..3014)
                     /locus_tag="VN034_09000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWV60778.1"
                     /translation="MTTKATASNEPVGRLLGWLGLRHARGSAPDGGLAPPDPGRPRHL
                     AREARYELAASITSFLVDNDLDVSPTNLLLAHGAFSGRNPRLARQIVAQAQTAEGISQ
                     KWLNELDDQEAGQPDRVELDRLMTRLETNIEAFQANTKEARTVTSDYGTELEQHVTDL
                     EQLEKTGRIVSSLADLAKVMLDRTRKAEDDMRKSEDEAKALRRSLERAKRDAELDYLT
                     GLPNRRAFEVLLDRHHKEARAAVEPLSVAFCDIDEFKKVNDLHGHEAGDRVIKAIADT
                     LARISNDHCHVARHGGEEFVMLFRGLTPTEAAAKLDDAREALADRRLINRKTDEPFGQ
                     ITFSGGVADVFGYGDTRAALKAADEALYKAKAAGRNCIRIADPA"
     gene            3221..>4261
                     /locus_tag="VN034_09005"
     CDS             3221..>4261
                     /locus_tag="VN034_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HWV60779.1"
                     /translation="MLGWILFARDLDPALPEVPEILRFQESAAARGIELHVLKPHNFD
                     IVAAPVDGWSVHYEGHSLERPDFILCRTGAETDYFTLAVLRHFERRGVRLINGPEAID
                     LVADKLHTMQRLARAGLPIPATILGKFPIGVDVVEEELGFPLIVKTLRGTRGTGVLKC
                     EDRSQFEDLAGLLESAEAKADFLFQHYVRSSHGRDVRVLVIGGRVIAAMERRSPAGHF
                     KSNVSLGGMGIAYTPTGEMADLAAHAADTLGLEVAGIDILFDDEGYRICEANSAPGFQ
                     GLERACGIDVPDAIFDWIEASHAAEGRPIRGVTGDALIDLVFGGRLSLRSIGDRLDEP
                     RAFARAVGVRLVG"
BASE COUNT          645 a         1312 c         1472 g          832 t
ORIGIN      
        1 ggtcgaatcc gcgccaatcg cgagccccgg tggcgcgctg gcggcgcgat cggtcgacgt
       61 gcgcgcgcag gaggacggca agagcttcac ttggaccggc ccggcgacgc tcgaattgac
      121 cggaccctat gccgacctgt cgcgccaact gaacaacagc ttcgcgcttc gcgtcgactg
      181 gcggatcgac gccgccggcg gcgcgcctgt gagcctcgcg ctcggcggca aggcgttcga
      241 catttcggca gcggtcaagg cggcggcgca gggccaggtt tcgacgatca aaatcccgct
      301 gcgctgcttc gccgatgcgg gggccaatct gcgccaggtc gacaatgcgg tgacggtccg
      361 tgccgacaag ggctttgccg caacgctgct gaatacgaat gtcgaggcgg tcggcgagaa
      421 tctgccttgc ccgcccgccg caaagtgaca agaccgggcg cgggttagcc tgaaaaaagg
      481 ttggggagag tgaaatggca ttagcgccga atgtcgcaac gagtgcggag ccgaacgcgg
      541 ccggacagcc gggcaggagc ggtcgcagca cccgggtggt gctgatcctc gccttcgcgg
      601 tgtttttcct gcttggcggg gtgacgaata tcaacgacct gctcgtcggc aagttcaagc
      661 cgatgttcca cctgacgcat gccgaggcga acctcgtgca gatggcgttc tttatcgctt
      721 atggcgcctt ttcgatcccc gccgggatca tcatgtcgaa gctcggctat attcgcacct
      781 tcgtgctcgg cttcatcatc gtagcggccg ccgcttttct cttcattccg gcttcggcgg
      841 cggcgtccta tcccggcttc ctcgcggcgc tcttttgcat cggtgcgggg atcaccgttc
      901 tgcaggtggc gatgaatccg gtgatcacca ccttggggcc ggtcgagact tcgcacagcc
      961 ggttgacctt tgcacaggcg ttcaattcga tcggcgtgtt cctgatggtc tatggcggtg
     1021 ccgccttcct gctcggcgat gagcagccga tcgatgccga gacctctacc gaggcgcaaa
     1081 ttcaggccta tcgcgttgcc gagggcgcat cgatcggcac cgcctatatg tggctcggcg
     1141 tgctgatgct ggtgatcgcg gcggtgttct ggatgtatcg gtcggcgatc gaccacgcga
     1201 agaccgacga tgtgaagctg gagggcacct ggggcctctt gacccacaac cgccgcgtcc
     1261 agttcggcgc gctgtgcatc ttcgtctatg tcggcgccga agtggcgatc ggatcgaacc
     1321 tgatggccta tctcgccgaa ccgagcgtga tggggctggg cctccaggcg gcgggcaagc
     1381 tggttgcgat gtactggctg ggcgcgctgg tcggccggtt gctcggcggc ttcatcctgc
     1441 ggctggccaa gccgggacgg gtgctcacga cctttgcggc cggcgcgatt tcgctgatca
     1501 tcctgtcggc ggtgacgacg ggggcgctgt ccggctgggc gctgattctg gtcggtttct
     1561 gcaattcgct gatgttcccg acgatcttca gcctcgcgac cgagggactc ggcgaacgga
     1621 cgccacaggg gtcgggtatc atgtgcaccg cgatcatcgg cggggcgatc gtcccatttc
     1681 tgttcggaac ggtggccgac cttagcggtg acatccgtat tgcattggcg gtgccgctga
     1741 tctgctatgc gatcattgcg agtttcggcc tgtgggcggc gtcgcgtccc acaaccgcgt
     1801 aacgataaag ccgcggacga tcggctctgg tgcacgatcg tccgcggctc ccccgccccc
     1861 tcgcgggaat aggtgtttca ggccgggtcg gcgatgcgga tgcagttccg tcccgcagcc
     1921 ttggctttat agagcgcctc gtccgccgcc ttgagcgcgg cgcgggtgtc gccatagccg
     1981 aaaacgtcgg cgaccccgcc cgagaaggtg atctggccga aaggctcgtc ggtcttgcga
     2041 ttgatcagcc gccggtcggc gagcgcctcg cgcgcgtcgt cgagcttcgc ggcggcctcg
     2101 gtcggggtca ggccgcggaa gagcatgacg aattcctcgc cgccgtggcg cgcgacatgg
     2161 caatggtcgt tcgagattcg cgccagcgtg tcggcgatcg ccttgatcac gcggtcgccc
     2221 gcctcatgac cgtggaggtc gttgaccttt ttgaactcgt cgatgtcgca aaaggcgacg
     2281 ctcagcggtt cgacggcggc gcgggcttcc ttgtggtggc ggtcgagcag gacttcgaat
     2341 gcgcggcgat tgggcaggcc ggtcagataa tcgagctcgg cgtcacgctt ggcgcgttcg
     2401 aggctccggc ggagcgcctt cgcctcgtcc tcgctcttgc gcatatcgtc ctcggccttg
     2461 cgcgtgcgat cgagcatcac tttggcgagg tcggcgaggc tggaaacgat gcggccggtc
     2521 ttttcgagct gttcgagatc ggtgacatgc tgttcgagct cggtcccata gtcgctcgtg
     2581 acggtccggg cttccttggt gttcgcctga aaggcctcga tattggtttc gagccgcgtc
     2641 atcagccggt cgagttcgac gcgatcgggc tgacccgctt cctggtcatc gagttcgttg
     2701 agccatttct ggctgatgcc ttcggcggtc tgcgcctgcg cgacgatctg gcgcgcgagc
     2761 cgcgggttgc gccccgaaaa ggcgccatgc gcgagcagga ggttcgtcgg cgacacgtcg
     2821 aggtcattgt cgacgaggaa agaggtgatc gaagcggcga gttcgtagcg cgcctcacgc
     2881 gccagatggc gcgggcggcc ggggtcgggg ggcgcaaggc ctccatcggg ggcgctgccg
     2941 cgtgcgtggc gaagccccag ccaacccagg agccgcccta ccggctcgtt cgatgctgtc
     3001 gctttggtgg tcatgcgagc tttaacgagc gtcgccgcgc aggtttaagg ccgatgcacc
     3061 ctatggcagc aggattttcc aaccgaatcg gacctgttcc ggcgacgatc ggtgcaatcg
     3121 gcgcgtcaaa acagcgattt agcacaccgg atcgcgtgaa tgattgcgcg agcgacgcct
     3181 tttctggcac ttagcggggc gattttcgaa ggagcgagtt ttgctgggct ggattttatt
     3241 cgcgcgcgac ctcgaccccg ccttgcccga ggttcccgag attctgcgct ttcaggaaag
     3301 cgccgcggcg cgcggaatcg agctccacgt cctgaagccg cacaatttcg atatcgtcgc
     3361 ggcgccggtc gacggctggt cggtccatta tgagggtcat tcgctcgaac ggcccgattt
     3421 catcctgtgc cggacaggcg cggaaaccga ctatttcacg ctcgcggtgc ttcgccactt
     3481 cgagcgtcgc ggcgtgcggc tcatcaacgg ccccgaggcg atcgaccttg ttgccgacaa
     3541 gctgcacacg atgcagcggc tcgcgcgcgc cgggctgccg atccccgcga cgatcctcgg
     3601 caaattcccg atcggggtcg atgtggtcga ggaggaactg ggttttccgc tgatcgtcaa
     3661 gacgctgcgc ggcacgcgcg ggacgggcgt cctgaaatgc gaggatcgca gtcagttcga
     3721 ggatcttgcg gggctgctcg aaagcgccga agccaaggcc gatttcctgt tccagcatta
     3781 tgtccggtcg agccacggcc gcgatgtgcg ggtgctcgtg atcggcgggc gcgtgatcgc
     3841 ggccatggag cggcgctcac ccgcagggca tttcaagtcg aacgtgtcgc tgggcggcat
     3901 ggggatcgca tacacgccga cgggcgagat ggccgatctt gccgcgcacg ccgccgacac
     3961 gctggggctg gaggtcgcgg ggatcgacat tttgttcgac gacgaagggt accggatctg
     4021 cgaggcgaac agcgcgccgg gattccaggg cctcgagcgc gcatgcggga tcgacgtgcc
     4081 cgatgcgatc ttcgactgga tcgaggcgag tcatgccgcc gaagggcggc cgatcagggg
     4141 ggtaaccggc gacgcgctga tcgacctcgt cttcggcggc cggctcagcc tgcgctcgat
     4201 cggcgaccgg ctcgacgaac cgcgcgcttt cgcgcgcgcg gtcggcgtcc ggctggttgg
     4261 c
//
LOCUS       DATMSU010000329         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_15177, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000329 DATMSU010000000
VERSION     DATMSU010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15177"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2331)
                     /locus_tag="VN034_09010"
     CDS             complement(<1..>2331)
                     /locus_tag="VN034_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWV60780.1"
                     /translation="PTDHVVANPQGQPDGTIEGGAFDDTLVGDPGSVTITEGQQANIV
                     LVLDSSGSMTTQIDFGNGTISRMQALKNGVNALIDSLSQSGAKDVRITVIDFDSDGDN
                     LGTFDLIVNGVVQAGEVDDAKDAVNGMSASGSTNYEDGLQDALSWINGGNGIAGADVN
                     KVVFVSDGNPTVWNNGGNGSDTDATNVQNSMNQVLGSDGTNEPELILATGYSIDSVGV
                     NVNPTLLARLSDVEDGNASGGTGSATNATSAEQLAAVLQVLGGSTDLAAAGDDTINGG
                     QGADIIFGDVLYTDTLAANLGVNLAPGSGWAVFQTLEGRVNAESIDPAGNGADWNRQD
                     TIAYVRANYTELAQESGRTGGNDTISAGGGNDVILGQEGNDTISAGGGDDLIAGGTGR
                     DVMTGGAGSDSFVFNAGESRPVLGGSGNAGTITGHDVITDFDLATDILNLPGTPVPDG
                     DGPVNGANSDLTIGGQPIRSHSVTGGVISFDDNNSFGTALALSSDARVAAAVDYLQRN
                     DLGPGGTTVAFVAGTRTFVYQQVGTDPDPANDILVELQGVTITNLNTLIGTRVAPVTL
                     DLDGDGLDFVSSAAGAAFDHDGDGVREATSWAGRGDGILVRDANGDGIANDGGEISFA
                     VAGSTDLEGLGLQYDSNGDGQLSAADTEFASFGVWQDANGDGVTDAGEFRSLADLGIA
                     SIALTSDGKAYVAGDGEVIVHGEASFTRTDGSTAAVGDVSFITPRAANDIDRTAGNQA
                     LAGSLVAASLVAIAHDVRPDGPVTDRELIEHAAATDV"
BASE COUNT          338 a          803 c          762 g          428 t
ORIGIN      
        1 gacatcggtc gccgcggcat gttcgatcag ttccctgtcc gtcaccgggc catccggccg
       61 cacgtcgtgc gcgatggcga cgagcgaggc cgcgaccagc gaaccggcga gcgcctgatt
      121 gcccgcggtc cggtcgatat cgttcgcggc gcgcggggtg atgaacgaca cgtcgccgac
      181 cgcggcggtg ctgccgtcgg tgcgcgtgaa gctcgcctcg ccatggacga tcacctcgcc
      241 gtcgcccgcg acataggctt tgccgtcgga ggtcagcgcg atgctagcga tgccgagatc
      301 ggcgagcgag cggaactcgc ccgcgtcggt gacgccgtcg ccattcgcat cctgccacac
      361 gccgaagctc gcgaattcgg tgtcggcggc gctgagctgg ccgtcgccat tgctgtcata
      421 ttgcaggccc agcccttcga ggtcggtcga gcccgcgacc gcgaagctga tctcgccgcc
      481 gtcattggcg atgccgtcgc cattggcgtc gcggacgagg atgccgtcgc cgcggcccgc
      541 ccacgacgtc gcctcgcgca cgccgtcgcc gtcatgatcg aaggccgcgc ccgcggcgga
      601 ggaaacgaaa tcgagcccgt cgccgtcaag gtcgagcgtc accggcgcga cgcgcgtgcc
      661 gatcagcgtg ttcaaattgg tgatcgtgac gccctgcagt tcgacgagga tgtcgttcgc
      721 cggatcggga tcggtcccca cctgctgata gacgaaggtc cgcgttcccg cgacgaaagc
      781 gacggtcgtt cccccggggc cgaggtcgtt gcgctggaga tagtcgaccg ccgccgcgac
      841 ccgcgcgtcg ctcgacagag caagcgccgt gccgaagcta ttattgtcgt cgaagctgat
      901 gacgccgccc gtgaccgaat gcgaccggat cggttggccg ccgatcgtca aatccgaatt
      961 ggcgccgttg accggcccgt cgccatcggg gaccggcgtt ccgggcaggt tcaatatgtc
     1021 ggtcgccaga tcgaaatcgg tgatcacgtc gtgcccggta atcgtcccgg cattgccgct
     1081 gccgcccagc accggacggc tttcgcccgc gttgaagacg aagctgtcac tccccgcgcc
     1141 gccggtcatg acgtcgcggc ccgtgccgcc ggcgatcagg tcgtcgccgc cgcccgcgct
     1201 gatcgtatca ttgccttcct gaccgaggat gacgtcgttc ccgccgccgg cactgatcgt
     1261 gtcattgccc cccgtccggc cgctttcttg cgccagttcc gtatagttgg cgcggacata
     1321 ggcgatcgtg tcctggcgat tccagtcggc gccattgccc gcggggtcga tcgattcggc
     1381 gttcacgcgg ccttcgagcg tctggaacac cgcccagccc gatcccggcg caaggttgac
     1441 gccgagattc gccgcgagcg tgtcggtgta gagcacgtcg ccgaaaatga tgtcggcgcc
     1501 ttgtccgccg ttgatcgtat cgtcgcccgc ggcggcgagg tcggtcgagc cgccgaggac
     1561 ctgaagcacc gccgcaagct gttcggccga ggtcgcgttg gtcgcgctgc cggtgccgcc
     1621 cgaggcattg ccgtcctcga catcgctgag gcgcgcgagc agcgtcggat tgacgttgac
     1681 gccgaccgaa tcgatcgaat agcccgtcgc gagaatcagt tccggctcgt tcgtgccgtc
     1741 ggagcccagc acctggttca tcgaattctg gacattggtc gcgtcggtgt cgctgccgtt
     1801 gccgccattg ttccagacgg tcggattgcc gtccgacacg aacacgacct tgttcacgtc
     1861 ggcgccggcg atgccgttgc cgccgttgat ccagctcagc gcgtcctgca gcccgtcctc
     1921 ataattggtg ctgcccgacg ctgacatgcc gttaacggca tccttcgcgt catcgacctc
     1981 gcccgcctgg acgacgccgt tcacgatcag gtcgaacgtc cccagattgt cgccgtcgct
     2041 atcgaaatcg atcaccgtga tgcgcacgtc cttcgcgccc gattgcgaga ggctgtcgat
     2101 cagcgcgttc acgccgttct tgagcgcctg catccgcgaa atcgtgccgt tgccgaagtc
     2161 gatctgcgtc gtcatgctgc ccgagctgtc gagtacgagc acgatattcg cctgctgccc
     2221 ctcggtgatc gtcaccgagc cggggtcgcc gacgagcgtg tcgtcgaagg cgccgccctc
     2281 gatcgtgccg tcgggctggc cctgcgggtt ggcgacgaca tggtcggtcg g
//
LOCUS       DATMSU010000330         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_91219, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000330 DATMSU010000000
VERSION     DATMSU010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91219"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VN034_09015"
     CDS             <1..1375
                     /locus_tag="VN034_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV60781.1"
                     /translation="LVLQGVTEPYRMLTARAEYRLRLRAADLPMARIKTGTPPRLDGR
                     TIDWARLDEQASDSAEGASWTCSTWNSERTVPQIFCAITRTNAQAHAIIAANLDRSPL
                     FTGAIGAAGPRYCPSIEDKIHRFADRDGHQVFLEPEGLDSFLVYPNGISTSLPADVQL
                     AMLRTMEGLEAVEMVVPGYAVEYDHIDPRALDRTLQVRAIPGVWCAGQINGTTGYEEA
                     AAQGLVAGANAALSVQGREPLILDRSESYIGVMVDDLVLQGVTEPYRMLTARAEYRLR
                     LRADNAATRLTPKGIALGLVGPATAELFAARMAERARAEALLDVAVSTADYAAVGLAL
                     PGDGIARKRIDLLRYPDMTMARLALLAPELDAIHPAILSEVAEDAHYAPYIARQDAEL
                     RSLAANEAILLDPALDYGAIGGLSREMVERLTKARPETLGQAARIDGVTPAALTAIMV
                     HSRRRAA"
     gene            1380..1670
                     /locus_tag="VN034_09020"
     CDS             1380..1670
                     /locus_tag="VN034_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60782.1"
                     /translation="MAFPRKILLRSPVSDEALLDAFVEQCLHDKVSIIAVVGADSVRI
                     EDIIDEIVVGDGSDTSRFVCTTSDPDESLEEVLNMLETWEMERADPVLEVRL"
     gene            1892..2560
                     /gene="rsmG"
                     /locus_tag="VN034_09025"
     CDS             1892..2560
                     /gene="rsmG"
                     /locus_tag="VN034_09025"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWV60783.1"
                     /translation="MMSSHGGILQNEEEARAWIAEALSPTAGQWDRLDRFASLLIAEN
                     AQQNLIAASTIPTIWVRHIADSAQLLSFDAREGEGLWVDLGSGPGLPGLVVAILSARP
                     MLLVESRKRRCEFLRAVADELGLGHVEVAEAPLERVETRPAATISARAFAPLDKLIGL
                     STRFSTESTRWLLPKGRNAVKELALLPEAWQRMFHVEQSRTDTDSQILVGQGRIGAKQ
                     RGKR"
     gene            2557..3339
                     /locus_tag="VN034_09030"
     CDS             2557..3339
                     /locus_tag="VN034_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV60784.1"
                     /translation="MIRIAVANQKGGVGKTTTAINLATALAATGWRTLIIDLDPQGNA
                     STGLGIKQSQREFSSYELLRGDATVAECAIPTAVPRLDIVSATVDLSGAEIELIEFQD
                     RLHRLQQALSGAEAGQWDICLIDCPPSLGMLTLNALIAAESLIVPLQCEFFALEGLSQ
                     LLTTVERVRERFNQKLSILGVALTMFDRRNRLTDQVSDDVREVLGPVVFETVIPRNVR
                     LSEAPSHGLPALIYDHRCAGSAAYIALARELIDRLPEIRKAA"
BASE COUNT          602 a         1021 c         1197 g          609 t
ORIGIN      
        1 tctggtgctg cagggggtga ccgagccgta ccggatgctc accgcgcgcg ccgaatatcg
       61 gctgcggctc cgcgccgccg acctgccgat ggcgcgcatc aagacgggga cgccgccaag
      121 gctcgacggg cggacgatcg actgggcgcg gctagacgag caggcgtcgg acagcgccga
      181 gggcgcgagc tggacctgtt ccacgtggaa cagcgaacgc acggtgccgc agattttctg
      241 cgcgatcacg cgcaccaatg cccaagccca tgcgatcatc gcggcgaatc tcgatcgctc
      301 gccgctgttc acgggcgcga tcggggcggc ggggccgcgc tattgcccgt cgatcgagga
      361 caagatccac cgcttcgccg accgcgacgg gcatcaggtg tttctcgaac ccgaggggct
      421 tgacagcttc ctcgtctatc cgaacgggat ttcgacctcg ctccccgccg acgtccagct
      481 cgcgatgctg cgcacgatgg aggggctcga ggcggtggag atggtcgtgc cgggctatgc
      541 ggtcgaatat gaccatatcg atccgcgcgc gctcgaccgg acgttgcagg tccgcgcgat
      601 tccgggcgtg tggtgcgcgg ggcagattaa cggcacgacg ggatatgagg aggcggcggc
      661 gcaggggctg gtcgcggggg ccaatgcggc gctcagcgtt cagggacgcg aaccgctgat
      721 ccttgatcgc agcgaatcct atatcggggt gatggtcgac gatctggtgc tgcagggggt
      781 gaccgagccg taccggatgc tcaccgcgcg cgccgaatat cggctgcggc tccgcgccga
      841 caatgccgcg acgcggctga cgccgaaggg gatcgcgctt gggctggttg gtccggcgac
      901 ggcggaattg ttcgcggcac ggatggccga gcgggcgagg gccgaagcct tgctcgatgt
      961 tgcggtgagc accgccgatt atgccgcagt cgggctggcg ttgcccggcg acgggattgc
     1021 gcgcaagcgg atcgacctgc tgcgctatcc cgacatgacg atggcgcggc tcgcgctcct
     1081 cgcgcccgaa ctcgacgcga tccacccggc gattctgtcg gaggttgccg aggatgcgca
     1141 ttatgcgccc tatatcgctc ggcaggatgc cgaactgcga tcgctcgcgg cgaatgaggc
     1201 gatcctgctc gaccctgcgc tcgattatgg cgcgatcggg ggattgtcgc gcgagatggt
     1261 cgagcggctg accaaggcgc ggcccgagac gctgggacag gcggcgcgga tcgacggggt
     1321 gacgccggcg gcgctgaccg cgattatggt gcacagccgg cggcgcgcgg cttgagggaa
     1381 tggcatttcc gcgcaagatc cttttacgct cacccgtttc ggatgaagca ctgctagatg
     1441 ctttcgtcga acaatgtctg catgacaagg tgtcgataat cgccgttgtt ggcgcggatt
     1501 ccgtccgaat cgaagatatt attgatgaaa tcgtggtggg cgacggctcc gacacaagcc
     1561 ggtttgtttg taccacatcc gatcccgacg aatctcttga agaggtcctc aacatgctcg
     1621 agacttggga gatggagcgt gctgatccgg ttctggaagt tcgcctttag gagccatccg
     1681 tatccgccgt caccccccgg acccttcgac aagctcagga caggcttgat ccggaatcca
     1741 ttctcgcgcc gtcgttgtgg accccggatc aagtccgggg tgacgaggaa gggaagggct
     1801 tcaatgtttg ggcaggaacg caaaggcatg agggttgagc gggactctcg cggaagagcg
     1861 ttcgcaggaa tggcgaaatg aaagacaggc catgatgagt tcccacggcg ggattttgca
     1921 gaatgaggaa gaagcgcgtg cctggatcgc ggaggcgctt tcgccgacgg ccggtcaatg
     1981 ggaccgcctt gatcgcttcg cttcgctgct catcgccgag aatgcgcagc agaatctgat
     2041 cgcggcgtcg acgattccga cgatctgggt gcggcatatc gccgatagcg cgcagctgct
     2101 gtcgttcgat gcgcgcgagg gcgaggggct ttgggtcgat ctggggagcg ggccggggtt
     2161 gcctgggctg gtcgttgcga tcctcagcgc acggccgatg ctcctcgtcg agtcgcgtaa
     2221 acggcggtgc gagttcttgc gcgcggtcgc ggacgagttg gggctggggc atgtcgaggt
     2281 tgccgaggcg ccgctcgaac gggtcgaaac gcggcccgcg gcgacgatca gtgcgcgggc
     2341 tttcgctccg ctcgacaagc tgattggctt atccacccgt ttttccaccg aatcgacgcg
     2401 ctggctgctg ccaaaggggc gaaacgcggt taaggaactg gcattgctgc ccgaggcatg
     2461 gcagagaatg ttccacgtgg aacaaagccg caccgacacc gacagtcaga ttcttgtggg
     2521 tcaggggcgg atcggcgcaa aacagcgagg aaagcgatga tccgcattgc cgtggcgaac
     2581 cagaagggcg gggtcggcaa gacgacgacc gcgatcaatc tggcgaccgc gctcgcggcg
     2641 accggctggc ggacgttgat catcgacctc gatccgcagg gaaacgcctc gaccgggctc
     2701 gggatcaagc agtcgcagcg cgaattttcg agctatgagc tgcttcgcgg cgacgcgacg
     2761 gtcgccgaat gcgcgattcc gaccgcggtg ccgcggctcg acatcgtttc ggcgacggtc
     2821 gacctgtcgg gggccgaaat cgagctgatc gaatttcagg accggctgca ccgcttgcaa
     2881 caggcgctgt cgggcgccga agcggggcag tgggacatct gtctgatcga ttgtccgccg
     2941 tcgctgggga tgttgacgct caacgcgctg atcgccgccg aatcgctgat cgtgccactc
     3001 cagtgtgaat ttttcgcgct cgaaggattg agccagctgc tcaccacggt cgagcgcgtg
     3061 cgcgagcggt tcaaccagaa attgtcgatc ctcggcgtcg cattgacgat gttcgaccgg
     3121 cgcaaccggc tgaccgacca ggtgtccgac gatgtccgcg aagtgctcgg ccccgtggta
     3181 ttcgaaacgg tgatcccgcg caacgtgcgc ctgtccgaag cgccgagcca cggccttccg
     3241 gcgctgatct acgatcatcg ctgcgccggg tcggccgcct atatcgcgct cgcgcgcgag
     3301 ctgatcgacc ggctccccga gattcggaag gcagcttaaa tgtctgataa tgataaagaa
     3361 agtggtccaa cgccgatccg caaccgccca tcggggctgg gccgcgggct gaacgcgctg
     3421 ttcggcgac
//
LOCUS       DATMSU010000331         4173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_176286,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000331 DATMSU010000000
VERSION     DATMSU010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176286"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..548)
                     /locus_tag="VN034_09035"
     CDS             complement(162..548)
                     /locus_tag="VN034_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HWV60785.1"
                     /translation="MPMMGPAAATPDAYIAALSGWQRERCEMMRAAILRAASFQETIK
                     WTNLVFMANGPCILIRAEEHRVLLGFWRGKRLREVEPRIKASGKYELGNLVMTEATEV
                     TAEEVSRLAAEAFRLNAELGNPGARA"
     gene            complement(673..1686)
                     /locus_tag="VN034_09040"
     CDS             complement(673..1686)
                     /locus_tag="VN034_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWV60786.1"
                     /translation="MTAEIHQLHGQRPPSLDPMMALVAADMNEVNAVILDRMQSEVPL
                     IPELAGHLIAGGGKRMRPMLTLACGKLLDYPGRRHCKLAAAVEFIHTATLLHDDVVDK
                     SDLRRGRKTANMIWGNSASVLVGDFLFSRAFEVMVEDGSLKVLKILSRASAVIAEGEV
                     NQLSAQRRIETSEDQYLAIINAKTAELFAAACKIAAVVAERDEATEAALDAYGRNLGI
                     AFQLVDDAIDYVSDAETMGKGVGDDFRDGKVTLPVILAYARGSAEEREFWKQAIVGHR
                     TSDEDLAYATSLLHKHDTITDTLARARHYGQRAVDAIGGFRASQAKSALTEAVAFAVA
                     RAY"
     gene            1795..2082
                     /locus_tag="VN034_09045"
     CDS             1795..2082
                     /locus_tag="VN034_09045"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWV60787.1"
                     /translation="MDDQLSRYRQSIDNIDAALVYMLAERFKVTKAVGELKAREGLPP
                     ADPSREERQIERLRSLARDADLDPDFTEKFLRFIIDEVIRHHQQAKREQDA"
     gene            2079..2774
                     /locus_tag="VN034_09050"
     CDS             2079..2774
                     /locus_tag="VN034_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWV60788.1"
                     /translation="MSLDHPIFARWPAEHPERIQLFSAPTPNGVKAGIMLEETGLAYE
                     PHRIDIMANESHDPAFLALNPNGKIPAIYDPAGPGGKPLALFESGAILIYLADKSGKF
                     LPTDPNLRYETIQWVMWQMGGVGPMFGQLGFFHKFAGREYEDKRPRDRYAAESARLLG
                     VLDGRLEGRTWIMGDDYSIADISLLGWVRNLIGFYEAREIVGFDRFAHVQRWLDAGLA
                     RPAVQRGLEVTAA"
     gene            2935..>4173
                     /locus_tag="VN034_09055"
     CDS             2935..>4173
                     /locus_tag="VN034_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV60789.1"
                     /translation="MRKLGMVAAATLALLGTTAHAKTTVVYAGRVITDAAKPAQGPTT
                     VIVTDDRITSITPGRAEAPAGAEIVDLGDKTLLPGLVDLHVHLTGDPGDDYRAAAVDP
                     DEWGVVVGAKNAAITLRAGFTTVREAGSAQYTAYALRRGTANGFIEGPRIVAAGPALS
                     IIGGHGDVTGFREDVHDVLDLGYTCTGALECAEKVRKASRAGADVIKITATGGVLSQQ
                     ARGLEGHFTSAELQSIADTAHSLGLKVMAHAHGAGGITASAAAGIDSIEHGTFADDAT
                     LKVMKAKGTYLVPTLMAFEGIRERLGKGIYTPTVEDKVRMTLNDVGKAVTRAKALGVP
                     IAFGTDAGVFEHGRNGGEFALLVKYGLTPREALASATTVAAKLLSMESEIGRIAPGMS
                     ADMIAVSGDPLADVTALEKVD"
BASE COUNT          722 a         1427 c         1350 g          674 t
ORIGIN      
        1 gagcagccac accgcgccct tgcaccaagg ctggtcgaga attgcgggcg cgacgcgcgt
       61 cgtcttgccc gcgcccggcg gcgcgacgac gacggcgttt gatttcagca ccagcgccgc
      121 catgatgtcg ggcagcaccg cgtcgatggg gagcatttgc atcaggcgcg cgccccggga
      181 ttgcccagtt cggcgttcag gcgaaaggct tcggcggcaa ggcgcgaaac ctcttcggcc
      241 gtgacctcgg tcgcttcggt catgaccaga ttgccaagct catatttgcc gcttgccttg
      301 atccgcggtt cgacctcgcg aagccttttg ccgcgccaga agccgagcag cacacgatgt
      361 tcctcggcgc ggatcaggat gcagggaccg ttcgccatga acaccaggtt ggtccatttg
      421 atcgtctctt ggaaagacgc cgcgcgcagg atcgccgcgc gcatcatttc gcagcgttcg
      481 cgctgccagc ccgacaacgc ggcgatatag gcgtcgggag tggctgccgc ggggcccatc
      541 atcggcattc gctcatcccc cctttttctt gtcttcgtgt ctttgcgcct ttgcgcgaaa
      601 ttcatccggt ttcgcgcaaa ggcgcaaaga cacgaagaag aaaacggatt ccgctgcgcg
      661 ggattgtcag aatcaatacg cccgcgcgac cgcgaaggcg accgcctcgg tcagcgccga
      721 tttcgcctgg ctggcgcgga agccgccgat cgcgtcgacc gcgcgctggc cgtagtggcg
      781 cgcacgcgcg agcgtgtcgg tgatcgtgtc atgcttgtga agcagcgagg tggcgtaggc
      841 gagatcctcg tccgacgtgc ggtgaccgac gatcgcctgt ttccagaatt cgcgctcctc
      901 ggccgacccg cgtgcatagg cgaggatcac gggcagcgtc accttgccgt cgcggaaatc
      961 gtcgccgacg cccttaccca tcgtttcggc gtccgaaaca tagtcgatcg catcgtcgac
     1021 aagctgaaaa gcgatgccga ggttgcggcc ataggcgtcg agcgcggctt cggtcgcttc
     1081 gtcgcgctcg gcgacgaccg cggcgatctt gcacgccgcc gcgaagagtt cggcggtctt
     1141 ggcgttgatg atcgcgagat actggtcctc gctcgtctcg atccgccgct gcgccgacag
     1201 ctggttgacc tcgccctcgg cgatcaccgc ggaggcgcgg ctgaggattt tcagcacctt
     1261 gagcgagccg tcctcgacca tcacctcgaa cgcgcggctg aacaggaagt cgccgacgag
     1321 caccgaggcg ctgttgcccc agatcatgtt cgcggtcttg cggccgcggc gcaggtccga
     1381 tttgtcgacg acatcgtcgt ggagcaaggt cgcggtgtgg atgaactcga ccgctgccgc
     1441 caatttgcaa tggcggcgcc ccggatagtc gagcagcttg ccgcacgcga gcgtcagcat
     1501 cggacgcatc cgcttgccgc cgcccgcgat cagatgtccc gcgagctcgg ggatcagcgg
     1561 cacttcggac tgcatccggt cgaggatgac cgcattcacc tcgttcatat cggcggcgac
     1621 gagcgccatc atcgggtcga gcgagggcgg cctctgccca tgcagctgat gaatttcggc
     1681 agtcatgacg cggcgaccct tggccgcgcg gtcgcgattt tgcaacgact ttcggcttgc
     1741 cagcagcccc cggccggtgc caagcgtgcc ggacataaga ggagccgcgt acccatggac
     1801 gaccagctga gccgttaccg ccagagcatc gacaatatcg acgcggcgct cgtctatatg
     1861 ctcgccgagc ggttcaaggt gaccaaggcg gtcggcgagc taaaggcgcg cgaaggcctg
     1921 ccccccgccg acccgagccg cgaggaacgc cagatcgagc ggctccgcag ccttgcccgc
     1981 gatgccgatc tcgaccccga cttcaccgag aaattcctgc gcttcatcat cgacgaagtg
     2041 atccgccacc atcagcaggc gaagcgcgag caggacgcat gagcctcgac cacccgatct
     2101 tcgcgcgctg gccggccgag catcccgagc gcatccagct cttttccgcg ccgaccccga
     2161 acggcgtgaa ggccgggatc atgctcgagg aaaccgggct tgcctatgag ccgcaccgga
     2221 tcgacatcat ggcgaacgaa agccacgacc ccgccttcct cgcgctcaat cccaacggca
     2281 agatcccggc gatctacgat ccggcgggac caggcggaaa gccgctcgcg ctgttcgaat
     2341 cgggcgcgat cctgatctat ctcgccgaca agagcggcaa attcctcccg accgatccga
     2401 acctgcgcta cgagacgatc cagtgggtga tgtggcagat gggcggcgtc ggcccgatgt
     2461 tcggccagct cggctttttc cacaaattcg ccggccgcga gtatgaagac aagcgcccgc
     2521 gcgatcgcta cgccgccgaa tccgcacgcc tgctcggcgt gctcgacggc cgcctcgaag
     2581 ggcgcacgtg gatcatgggc gacgattaca gcatcgccga catctcgctg ctcggctggg
     2641 tgcgcaacct gatcggcttt tacgaagcac gcgagatcgt cggcttcgac cgctttgcgc
     2701 atgtgcagcg ctggctggat gcggggctgg cgcgcccggc ggtgcagcgc gggctggaag
     2761 tgacggccgc ttaagggcgc gcggcggctt ccggccggta gcggccaggg caagccccct
     2821 cctcaagagg gggttggtca acgatagctc cccattcccg atgccgacgc tgcatccccc
     2881 ttgccctccc ccgcccgctc cgccacgatg gcgcgatcag acaggggaga cttcatgcgt
     2941 aaactgggaa tggtcgcggc agcgacgctg gcgctgctcg gaacgacggc gcacgcgaaa
     3001 accaccgtcg tctatgcagg ccgcgtcatc accgacgcag ccaagccggc acaaggcccc
     3061 acgaccgtca tcgtcaccga cgaccgcatc acctcgatca cccccggccg cgccgaagcg
     3121 cccgcgggcg ccgagatcgt cgaccttggc gacaagacat tgctccccgg cctcgtcgac
     3181 ctccatgttc acctgaccgg cgatcccggc gacgattacc gcgccgcggc ggtcgatccc
     3241 gacgaatggg gcgtcgtcgt cggggcgaag aacgccgcga tcaccttgcg cgcgggcttc
     3301 accacggtgc gcgaggcggg atcggcgcaa tataccgcct atgcgctgcg ccgcggcacc
     3361 gcgaacggct ttatcgaggg gccgcgcatc gtcgccgccg gccctgcgct gtcgatcatc
     3421 ggcgggcacg gcgacgtcac cggcttccgc gaagatgtcc acgacgttct cgatcttggc
     3481 tatacctgca ccggcgcgct cgaatgcgcg gaaaaagtgc gcaaggcctc gcgcgccggc
     3541 gccgacgtca tcaagatcac cgcgaccggc ggcgtgctgt cgcagcaggc gcgcgggctc
     3601 gaaggccatt tcaccagcgc cgagctgcag agcatcgccg acaccgccca ttcgctcggg
     3661 ctcaaggtga tggcgcacgc gcatggcgcc ggcggcatca ccgcctcggc cgcggcgggc
     3721 atcgacagca tcgagcatgg caccttcgcc gacgatgcga cgctgaaggt gatgaaggcc
     3781 aagggcacct atctcgtccc caccctgatg gcgttcgagg ggattcgcga acggctcggc
     3841 aagggcatct ataccccgac ggtcgaggac aaggtgcgca tgacgctgaa cgacgtgggc
     3901 aaggcggtga cgcgcgccaa ggcgctcggc gtgccgatcg ccttcggcac cgacgccggc
     3961 gtgttcgaac atggccgcaa cggcggcgaa ttcgcgctgc tcgtcaaata tggcctgacc
     4021 ccgcgcgagg cgctggcgag cgcgacgacg gtcgccgcga agctcttgtc gatggaaagc
     4081 gagatcggcc ggatcgcgcc gggcatgtcg gccgacatga tcgcggtcag cggcgacccg
     4141 ctcgccgatg tcaccgcgct cgaaaaggtc gac
//
LOCUS       DATMSU010000332         6181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_63214, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000332 DATMSU010000000
VERSION     DATMSU010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63214"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VN034_09060"
     CDS             complement(<1..955)
                     /locus_tag="VN034_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HWV60790.1"
                     /translation="MAANPDAIAFRGALGAFATGVTIVTTRDGAGVDYGLTANSFNSV
                     SLDPPMVLWSLARTSQNMEAFRGASHFAVHILAADQEALSNGFAKRGADKFAGVEVER
                     GEGDVPLLTGCTARFQCRTAYQYEGGDHIIFVGEVIAFDHEDRAPLLFHGGRYAMAAR
                     KAATLTACDPGAQPAGSFGEDFLGYLLGRAHFQLYGRMRDSLSEHHLDATDHFILSVL
                     GINDGRTAAEIEGVIGYTGAHISSDRIASLAARGIVRTDPADRLWLTDTGRRTLVELA
                     AAAKAAEEDALGAFDANETALLKHLLKHVIRSTDPGLPDPWA"
     gene            complement(1080..2222)
                     /locus_tag="VN034_09065"
     CDS             complement(1080..2222)
                     /locus_tag="VN034_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV60791.1"
                     /translation="MRIGKPLADVDELVTLLYRGPLEATPWLGFLEALSRRMRCASAA
                     ITLRLSRQGTPPLIIWGPPPVIDEREARRIHAVHAELGDLDPLRNALTKAGDIYRLSE
                     VISPGDLRANRFYREILQPYGIEYQLGMYISEPGGWEGNVGLTNGPDEEDFSDDDKAV
                     LLALRPHLEQSLALFSRIRRDETELQVMIDTLDRLTLCTFILDGAGRILRTNSAGRAL
                     VKSNSAVRAADDRLVLASKAGNAQLQQLIGKAIAARRDGGSFAEGLRVEADIDRHLGV
                     LVRTIETPSRYSNEAGPAAVVYVSGLDNNQPVERLVSQIFDLTPSEAHLAALLATGLS
                     LAEAAARLELTENTVRSYCKTILSKTGVSRQADLVRLILRSVAVLG"
     gene            2443..3993
                     /locus_tag="VN034_09070"
     CDS             2443..3993
                     /locus_tag="VN034_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HWV60792.1"
                     /translation="MADAKFTIPHRIFAAARAHGDAVAIRGEDGTEVRYRDLEPLVRA
                     AAKGFIAAGVAPGDRIAIWAPNATGWIIAALGAQTAGAAIVPLNTRLKGREAGYILRT
                     SGARLLLTVSDFLGTAYPQLLAGEDLPDLRGIVLIEGDAGGTPWRTFLDRGATLSDAA
                     VDARLDALGEDQVCDILFTSGTTGNPKGAVTTHGQNVRLYLAYGETLTYGPGDNFLII
                     NPFFHSFGYKAGWLVALMRGVTVLPHAVFHTDKVLERIERERVTLLPGPPTIFQSLLA
                     GPYRDHDISSLRVSITGAASVPVILIEEMRGKLGIDVVLTAYGLTETCGVVTMCGAGD
                     DAATIANTAGRPLDGIEVRLVDEAGEAVTVGEAGELLVRGPNVMRGYFNDPVATEAAI
                     DAEGWLRTGDIAVQDAHGNVKITDRAKDIFIVGGFNAYPAEIEGLLAANPAVMQSAVI
                     GVPDERLGEVPKAYVVLRPGVSATPEEIVAWCRENMANYKVPRRVEIVDALPVNAAGK
                     VQKFLLRG"
     gene            4267..5307
                     /gene="obgE"
                     /locus_tag="VN034_09075"
     CDS             4267..5307
                     /gene="obgE"
                     /locus_tag="VN034_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWV60793.1"
                     /translation="MHFLDQAKIFIKSGDGGPGAVSFRREKYIEYGGPDGGNGGKGGD
                     IVFEAVAGLNTLIDFRYTQHFKAKRGTPGAGRDRTGAGGPDLIIQVPIGTQILDDDEE
                     RSLLADLTKEGEQLVFLRGGDGGRGNASYKTSTNRAPRQHGPGWPGEEMWVWLRLKLL
                     ADAGLVGLPNAGKSTFINAVTNAQAKVGAYAFTTLRPQLGVVSHKGHEFVIADIPGLI
                     EGAAEGAGVGDRFLGHIERCRVLLHLVDANDEDVATSYRIVRDELEAYGADLIDKPVI
                     VALNKTDTLDDELIAALSAELEAESGHPVMALSGASGAGVPAVLDKLLEAIGHPEPGE
                     DESEESADWSPI"
     gene            5370..6170
                     /locus_tag="VN034_09080"
     CDS             5370..6170
                     /locus_tag="VN034_09080"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HWV60794.1"
                     /translation="MTRRHPLPALLLIALTAVAAPAQAQAQEGNESAEKRTRIILGPQ
                     LTPSWPGADKFSVGPYIDVTRTRDSEFEYEAPDESFGQPLVHTGDFAFGPALGFIGKR
                     KASDIGADLPKVGFTIEAGAFAQAYLTPALRIRAEGRKGLSGHKGWVGEVSADYIARQ
                     GDDWLFSIGPRVTLGDAKYHRAYFGVTPAAAATSGLPAYDPGGGVQSVGVAAGYHHML
                     GKNWGIAVYGRYDRLVGDAADSPVTRQLGSRNQPSVGVALSYTFGGDR"
BASE COUNT         1038 a         2070 c         2086 g          987 t
ORIGIN      
        1 ccgcccacgg atcaggaagc cccggatcgg tgctgcggat gacgtgcttg agcagatgct
       61 tgagcagcgc ggtctcattc gcgtcgaaag cgccgagcgc atcttcctcg gccgccttgg
      121 cggcagcggc gagttcgacg agcgtgcggc ggccggtgtc ggtcagccag agccggtcgg
      181 cgggatcggt ccggacgatg ccgcgcgccg cgagcgatgc gatgcggtcg gagctgatgt
      241 gcgcgccggt atagccgatc acgccttcga tctcggccgc ggtgcgtccg tcgttgatgc
      301 cgagcacgct caggatgaaa tgatcggtgg cgtcgagatg atgttcactc aggctgtccc
      361 gcatccgccc gtagagctga aaatgcgcgc ggccgagcag atagccgagg aaatcctcac
      421 caaagctgcc cgcgggctgc gccccggggt cgcaggcggt gagcgtcgcg gccttgcgcg
      481 cggccatcgc atagcggccg ccatggaaca gaagcggcgc gcggtcctca tgatcgaagg
      541 cgatcacttc acccacgaag atgatatggt cgccgccctc atattgatag gcggtgcggc
      601 actggaaacg cgcggtgcag ccggtaagca gcggcacgtc gccttcgccg cgttcgacct
      661 cgacgcccgc gaatttgtcg gcgccgcgct tggcgaagcc gttggagagc gcctcctgat
      721 cggcggcgag gatatgcacc gcgaaatgcg atgcaccccg aaacgcctcc atgttctgcg
      781 acgtgcgcgc gaggctccac agcaccatcg gcgggtcgag cgagaccgag ttgaagctgt
      841 tcgcggtcag cccgtaatcg acacccgcgc cgtcgcgcgt cgtgacgatc gtcaccccgg
      901 tcgcgaatgc gccgagcgcg ccgcggaagg caatggcgtc gggattggcg gccatgacta
      961 tttgctcctc atccggccgc gtctggtggg cgggtatcat aggaccctat cgcccggtct
     1021 cggccgcgca tcgctcaatt ggactagatg tgaagcccgg attgcggctt tggtctgcgt
     1081 cagccgagaa cggcgacgct gcgcaggatc aggcgaacaa ggtccgcctg gcggctgacc
     1141 ccggtcttgc tgaggatcgt cttgcaatag ctgcgcaccg tattctcggt cagctccagc
     1201 ctagcggccg cctcggcgag gctgagcccg gtcgcgagca gcgccgccag atgcgcttcg
     1261 gacggcgtga ggtcgaagat ctggctgacc agccgttcga ccggctggtt gttgtcgagc
     1321 cccgaaacat agaccaccgc cgccggcccg gcctcgttgc tgtagcgcga gggggtttcg
     1381 atcgtgcgca cgagcacgcc cagatggcgg tcgatgtcgg cctcgacgcg cagcccctcg
     1441 gcgaagctgc caccgtcgcg ccgcgccgcg atcgccttgc cgatcagttg ctggagctgg
     1501 gcattgcccg ccttgctcgc gagcaccaga cggtcgtcgg cggcgcgcac cgcgctgttc
     1561 gatttgacca gcgcgcggcc ggcgctgttg gtgcgcagga tgcggcccgc gccgtcgagg
     1621 atgaaggtgc aaagcgtcag ccggtcgagc gtgtcgatca tcacctgaag ctcggtctcg
     1681 tcgcggcgaa tgcgcgagaa gagcgcgagc gactgttcga gatgcgggcg gagcgcgagc
     1741 agcaccgcct tgtcgtcgtc gctgaaatcc tcttcgtcgg ggccgttggt caggccgaca
     1801 ttaccctccc accccccggg ctcggagatg tacatgccga gctgatattc gatgccataa
     1861 ggctgcaaaa tctcgcgata gaagcgattg gcgcgaaggt cgcccggcga aataacctcg
     1921 ctcagccggt agatgtcgcc ggccttggtc aaggcgttgc gcagcggatc gagatcgccg
     1981 agttcggcat ggaccgcgtg aatccgccgc gcctcgcgct cgtcgatcac gggcggcggt
     2041 ccccagatga tcagcggcgg ggtcccttga cggctgagac gcagggtgat cgcggcgctg
     2101 gcgcagcgca tccgccggct caatgcttcg aggaagccga gccatggcgt cgcctcgagc
     2161 ggcccgcgat agagcagggt cacgagttca tcgacgtcgg ccagaggttt tccgatccgc
     2221 attccggctt cgcttcctgt tcgcgtccct gccctgttat taggggagtc ggcccccggt
     2281 cggcaatgaa ccgtccgggc cggggcggca aggggtggcg atccgatcgc gctccccccg
     2341 aataggggtt gtcgccgccc cgcccgcgcg taacgcagcg aggagcaatc gaccgggcgg
     2401 atggacggga tccatgagca ccggcgcagg agatgatctg ccgtggccga cgcgaagttc
     2461 acgatacccc acaggatatt cgccgcggcg cgcgcgcatg gcgatgccgt cgcgatccgg
     2521 ggcgaggacg gaaccgaggt tcgctaccgc gacctcgaac cgctggtgcg cgcggcggcc
     2581 aagggcttta tcgcggcggg ggtcgcccca ggggatcgga tcgcgatctg ggcgcccaat
     2641 gcgacgggct ggatcatcgc cgccctcggc gcgcagacgg cgggcgccgc gatcgtgccg
     2701 ctcaacacgc ggctgaaagg gcgcgaggcg ggctatatcc tgcgcaccag tggcgccaga
     2761 ctgctgctca ccgtttccga ttttctcggc accgcctatc cgcaattgct cgcgggcgag
     2821 gatctgcccg atctgcgcgg tatcgtgttg atcgagggcg acgcgggcgg cacgccctgg
     2881 cggactttcc tcgaccgcgg cgcgacgctt tcggatgcgg cagtcgacgc acgcctggat
     2941 gcgctgggcg aggatcaggt gtgcgacatc ctcttcacct cgggcaccac gggcaatccg
     3001 aagggtgccg tgacgacgca cggccagaat gtccggctct atctcgccta tggcgaaacg
     3061 ctgacctatg ggccgggcga caatttcctg atcatcaacc cctttttcca cagcttcggt
     3121 tacaaggctg gctggctggt cgcgttgatg cgcggcgtga ccgtgctgcc ccacgccgtg
     3181 ttccacaccg acaaagtgct cgaacggatc gagcgcgaac gggtgacatt gctccccggt
     3241 ccgccgacga tcttccagag cctgctcgcg ggtccttatc gcgaccatga tatttcgtcg
     3301 ctgcgcgtgt cgatcacggg cgccgcgtcg gtgccggtga tcctgatcga ggagatgcgc
     3361 ggcaagctcg gcatcgatgt cgtgctcacc gcctatggcc tcaccgaaac ctgcggcgtc
     3421 gtgacgatgt gcggcgccgg cgacgatgcc gcgacgatcg ccaacaccgc gggacgcccg
     3481 ctcgacggga tcgaggtaag gctggtcgac gaagcgggcg aggcggtgac ggtgggcgaa
     3541 gcgggcgaac tgctcgtgcg cgggcccaat gtcatgcgcg gctatttcaa tgatccagtg
     3601 gcgaccgaag cggcgatcga cgccgaaggc tggctgcgaa ccggcgacat tgcggtgcag
     3661 gacgcgcacg gcaatgtgaa gatcaccgac cgtgcgaagg acatcttcat cgtcggcggc
     3721 ttcaacgcct atccggccga gatcgaaggg ctgctcgcgg ccaatccggc ggtcatgcag
     3781 tcggcggtca tcggcgtgcc cgacgaacgg ctgggcgagg tgcccaaagc ttatgtcgtc
     3841 ctgcgccccg gcgtgagcgc cacccccgaa gaaatcgtcg cttggtgccg cgagaatatg
     3901 gcgaattaca aggtcccgcg gcgggtggag atcgtcgatg cgctgccggt gaatgcggcg
     3961 ggcaaggtgc agaagtttct gctcagggga taacgggttt cggccggtag cggacgtaaa
     4021 gatcctcccc ttcgcgtagc gattggggag gggaaccgcc gcgaagcggt ggtggagggg
     4081 tctggccttg accggcgcct gacggcgtcg acccctccgt cagccctgtg ggctgccacc
     4141 tccccattgc tccgcaaagg ggaggatcta acctccgctc accaccccga aacgggacat
     4201 cggccgctgc gcccggacgg cgacgctgcg ctggccaaat cgcgcctttc gccctaaagg
     4261 cccgccatgc acttccttga ccaagccaag attttcatca agtccggcga cggcggcccc
     4321 ggcgccgtgt cgttccggcg cgagaaatat atcgaatatg gcggccccga cggcggcaac
     4381 ggcggcaagg gcggcgacat cgtcttcgaa gccgtcgccg gcctcaacac gctgatcgat
     4441 tttcgctaca cccagcattt caaggcgaaa cgcggcaccc ccggcgcggg ccgcgaccgc
     4501 accggcgcgg gcggccccga cctcatcatc caggtgccga tcggcaccca gatcctcgac
     4561 gacgacgagg agcgcagcct gctcgccgat ttgaccaaag agggcgaaca actcgtattc
     4621 ctgcgcggcg gcgacggcgg gcgcggcaat gcgagctaca agacctcgac caaccgcgcg
     4681 ccgcgtcagc acggccccgg ctggccgggc gaggaaatgt gggtgtggct gcggctgaaa
     4741 ctgctcgccg acgcgggcct tgtcggcttg cccaacgccg gcaagtcgac cttcatcaac
     4801 gcggtgacca acgcgcaggc caaggtcggc gcctatgcct tcaccacgct ccgcccccaa
     4861 ctcggcgtcg tcagccacaa ggggcatgaa ttcgtcattg ccgacatccc ggggctgatc
     4921 gagggcgcgg cagaaggagc aggcgtcggc gaccgttttc tcggccatat cgaacgttgc
     4981 cgcgtcctcc tccacctcgt cgatgcgaac gacgaggatg tcgcaacgag ctaccgcatc
     5041 gtgcgcgacg aactcgaagc ctatggcgcc gacctgatcg acaagccggt gatcgtcgcg
     5101 ctcaacaaga ccgacacgct cgacgacgag ctgatcgcgg cgctgtcggc cgagctcgaa
     5161 gccgaaagcg gccacccggt gatggcgctc tcgggcgcca gcggcgcggg cgtgcccgcg
     5221 gtgctcgaca agctgctcga ggcgatcggc catcccgaac ccggcgaaga cgaaagcgaa
     5281 gaatcggccg actggtcgcc gatctaggcc ctgcccctcg gaacgaaacg ccccgcagcg
     5341 aatttcatgc ggaacaacaa ggatttcgca tgacccgacg gcacccgctt cccgccctgc
     5401 tcctcatcgc gctgacggcc gtcgccgccc cggcccaggc ccaagcccag gaggggaacg
     5461 aaagcgccga aaagcgcacg cgcatcatcc tcgggccgca actgacgccg tcgtggccgg
     5521 gcgcggacaa gttcagcgtc ggcccctata tcgacgtcac gcgcacccgc gacagcgagt
     5581 tcgaatatga ggcgcccgac gaaagcttcg gccagccgct cgtacacacc ggcgatttcg
     5641 ccttcggccc cgcgctcggc ttcatcggca agcgcaaggc gtccgacatc ggcgccgacc
     5701 tgcccaaggt cggcttcacg atcgaagcgg gcgccttcgc gcaggcctat ctgacgcctg
     5761 cgctccgcat ccgcgccgaa ggacgcaagg gcctcagcgg tcacaagggc tgggtcggtg
     5821 aagtcagcgc cgactatatc gcgcggcagg gcgacgactg gctcttttcg atcggcccgc
     5881 gcgtcacgct cggcgacgcc aaatatcatc gcgcttattt cggcgtgacg cctgcagccg
     5941 ccgccacctc gggcctgccc gcctatgatc cgggcggcgg cgtccagtcg gtcggcgtcg
     6001 ctgcgggcta tcaccacatg ctcggtaaga attggggcat cgcggtctat ggccgctacg
     6061 accggctcgt cggcgatgcc gccgattcgc ccgtcacgcg ccagctcggg tcgcggaacc
     6121 agccgtcggt gggcgtcgcg ctcagctaca ccttcggcgg cgaccgctga accgccgacg
     6181 c
//
LOCUS       DATMSU010000333         1850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_185837,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000333 DATMSU010000000
VERSION     DATMSU010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185837"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1757)
                     /gene="ahpF"
                     /locus_tag="VN034_09085"
     CDS             complement(201..1757)
                     /gene="ahpF"
                     /locus_tag="VN034_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HWV60795.1"
                     /translation="MLDANTTAQLKTYLGNLRRPIELVASLDASPKSAEMRSLVEEVA
                     AQSDLVTARFDGNDARRPSFAIRADEGRAEAVFAGLPMGHEFTSLILALLHVGGHPPK
                     EEAELLDAVRALEGDFVFETFFSLSCQNCPDVVQALNIMAALNANIRHTAIDGALFQD
                     EVERRKVMAVPAVFLNGEPFGQGRMDLAQIVAKLDTGAVARAAEKIAAKEPFDVLVVG
                     GGPGGAAAAIYAARKGIRTGIVAERFGGQVLDTLGIENFVSVQHTEGPKLAAQLEAHV
                     KDYDVDVMNVQEAAELIPGGAHGLHEVKLKSGASVKGKTVILSTGARWRQMGVPGEAE
                     YRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGLVAHVTLIEFDGQLRADA
                     VLQTKLRSLPNVTIITSALTTEVLGNGEKVVGLRYRDRNKDEEHLVELEGIFVQIGLI
                     PNTEWLGNAVALSDRGEIEVDARGATSQPGIFAAGDVTTVPYKQIVIAMGEGSKASLS
                     AFDHLIRSSA"
BASE COUNT          312 a          606 c          605 g          327 t
ORIGIN      
        1 cccgatccgc gcgaaaggca tcatggccag cggcgcatga caaggccgca tcaaaggccg
       61 gacacatggc tgcaccaacc agcagatcgc atctacctag aaaccgcttg ccaacggagg
      121 gccgtccaca cacggccaaa acggctgcat tcgattctcg ggttccgagt ttagtgggag
      181 aaattatcgg ctcagtcaga tcaagccgag gagcggatga ggtgatcgaa cgcagagagc
      241 gacgcctttg atccttctcc catcgcgatg acgatctgct tgtacggcac cgtcgtcacg
      301 tcgcccgcgg cgaatatacc gggctggctt gtcgcgccgc gcgcgtcgac ctcgatctcg
      361 ccgcggtcgc tgagcgccac cgcattgccg agccattcgg tgttcgggat cagaccgatc
      421 tggacgaaaa tgccctccag ctcgaccaga tgctcctcgt ccttgttgcg gtcgcgatag
      481 cgcaggccga cgaccttctc gccattaccg agcacttcgg tggtgagcgc cgaggtgatg
      541 atcgtgacat tgggcagact gcgcagcttg gtctgcagca ccgcatcggc gcgaagctgg
      601 ccgtcgaatt cgatcagcgt cacatgcgcg acgagcccgg caaggtcgat cgccgcctcg
      661 acccccgagt tgccgccgcc gatcaccgcg acgcgcttgc ccttgaacag ggggccgtcg
      721 cagtgcgggc agtaagcgac gcccttgttg cgatattcgg cctcgccggg gacgcccatc
      781 tggcgccagc gcgcacccgt cgacaggatc accgtcttgc ccttcacgct cgcgccgctc
      841 ttgagcttca cttcgtgcag gccgtgcgcg ccgcccggga tcagctcggc ggcttcctgc
      901 acgttcatga cgtcgacgtc ataatccttc acatgcgctt ccagttgcgc ggcgagcttc
      961 ggtccctcgg tgtgctggac cgagacgaaa ttctcgatcc ccagcgtgtc gagcacctgc
     1021 ccgccgaagc gttccgcgac gatgccggtg cggataccct tgcgcgcggc atagattgcc
     1081 gccgccgcac cgccggggcc gccgccgacg acgagcacgt cgaacggctc cttcgctgcg
     1141 atcttctccg ccgcgcgcgc gaccgcgccg gtgtcgagct ttgcgacgat ctgcgcgagg
     1201 tccatgcggc cctgcccgaa gggttcgccg ttcaggaaga cggcgggaac cgccatcacc
     1261 ttacggcgct cgacctcgtc ctggaacaat gcgccgtcga tcgcggtgtg gcggatgttc
     1321 gcattgagcg ccgccatgat gttgagcgcc tgcaccacgt cggggcaatt ctggcacgag
     1381 agcgagaaga aggtttcgaa aacgaaatcg ccttcgaggg cgcgcaccgc gtcgagcagt
     1441 tcggcctctt ccttcggcgg gtggccgccg acatggagca gcgcgaggat cagcgaggtg
     1501 aattcatgcc ccatcggcag ccccgcgaac accgcttcgg cgcgtccctc atcggcgcgg
     1561 atcgcgaagg acgggcggcg ggcgtcgttg ccgtcgaacc gcgcggtgac gaggtccgac
     1621 tgcgcggcga cctcttcgac aaggctgcgc atctcggccg acttgggcga ggcgtcgagg
     1681 ctcgcgacca gctcgatcgg gcggcggagg ttgccgagat aggttttgag ctgggcggtc
     1741 gtattggcgt cgagcatcgg catgggagag cctttcggac aggagcaggg gttcgccgcg
     1801 gggaacggcg tcagtatctt ttgttttatg aaaagctggg accgggcggg
//
LOCUS       DATMSU010000334         2912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_81412, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000334 DATMSU010000000
VERSION     DATMSU010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81412"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VN034_09090"
     CDS             <1..852
                     /locus_tag="VN034_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HWV60796.1"
                     /translation="GRSSTDGTVRGGALPATWGRVFAENSEIKWDGDVAPSLDGDLSG
                     FQIGQDFAGFGDEGGTNIRLGAFVGRSSTDGTVRGGALGWNDLTVGQIDVKATSLSGY
                     ATLVGENGWYIDVVVMHSWFDGETAASSGEAIDVDGKGWTASLEAGYPLALSDNWTLE
                     PQAQILWQQVKLDDRADSFSTIDFATGDVLTGRAGLRLQGEYGGFQPYLHASLWHGFS
                     GDQTTRFGSDPIRTDLGRTEAEVGAGLIARLGTSVAVHVKGDYGFDADGPRSRRYGGT
                     VGLTISW"
     gene            complement(856..2079)
                     /locus_tag="VN034_09095"
     CDS             complement(856..2079)
                     /locus_tag="VN034_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV60797.1"
                     /translation="MQFDVVIVGAGHGGAQVAVMLRTQKFAGSIAIIGDEPELPYERP
                     PLSKEYFAGEKEFERIQLRPAKYWDEREVTMLLGRRVVSVDPAGHTVTTDGGETIGYG
                     KLVWATGGAPRMLPIPGGDLPGVQGVRTRADADAMKAASESAKQIVVIGGGYIGLEAA
                     AVLNKAGKKVVLLEALDRVLARVAGADLSRFYEKEHREHGVDLRLGVCVDAIEGDDHV
                     TGVRLADGEVIPADLVIVGIGIVPAIEPLTAAGADCANGVRVDPLCKTSLADIYAIGD
                     CASHANDFAEGAEIRLESVQNANDQANVVAKGIAGDEAPYHAIPWFWSNQYDLKLQTA
                     GLSTGHDQAVLRGDPANRSFSVVYLKAGKVIAIDCVNATKDYVQGRMIVTAGLRATAE
                     QLADTETPLKALLPA"
     gene            complement(2179..2463)
                     /locus_tag="VN034_09100"
     CDS             complement(2179..2463)
                     /locus_tag="VN034_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60798.1"
                     /translation="MHRFRIDAILDPQSLPRVANFFAQRAFVPSAKTMHLLPSHMRIE
                     VVVAGLETAQAAIIAAKLGEVVAVLRSEVEEIETAVFAADRAKPALTLVG"
     gene            complement(2463..>2912)
                     /locus_tag="VN034_09105"
     CDS             complement(2463..>2912)
                     /locus_tag="VN034_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid dehydrogenase"
                     /protein_id="HWV60799.1"
                     /translation="DVSPVRRDRLAAVHGAEVVDVSEIASVDADIFAPCALGGALDRD
                     TVAKLKAKLVCGAANNQLATPDVAGLLLDRGIAYAPDYVVNAGGIINVSAEYLGEDES
                     DVELRVAQIAPRVGDLLARAAREGRSPAIVADEMAEEVIAGAQREAA"
BASE COUNT          451 a         1016 c          959 g          486 t
ORIGIN      
        1 gggcgctcgt cgaccgacgg caccgtccgc ggcggcgcgc tgcccgcgac ctggggccgc
       61 gtctttgccg agaatagcga gatcaaatgg gacggcgacg ttgccccgtc gctcgacggc
      121 gacctgtcgg gctttcagat cggccaggat ttcgccggtt tcggcgacga gggcggcacg
      181 aatatccgcc tcggcgcctt tgtcgggcgc tcgtcgaccg acggcaccgt ccgcggcggc
      241 gcgctcggct ggaacgacct gaccgtcggc cagatcgacg tcaaggcgac gagcctttcc
      301 ggttatgcca cgctcgtcgg cgaaaacggc tggtatatcg atgtcgtcgt catgcacagc
      361 tggttcgacg gcgaaaccgc ggccagttcg ggcgaggcga tcgacgtcga cggcaagggg
      421 tggaccgctt cgctcgaagc gggctatccg ctcgcgctct cggacaattg gacgctcgag
      481 ccgcaagcgc agatcttgtg gcagcaggtg aagctcgacg accgcgccga cagcttttcg
      541 acgatcgact tcgcgacggg cgatgtgctc accggccgcg ccgggctgcg cctgcagggc
      601 gaatatggcg gcttccaacc ctatctccat gcgagcttgt ggcatggttt ttcgggcgat
      661 cagacgacgc gcttcggcag cgatccgatc cgcaccgacc tcggccgcac cgaggccgaa
      721 gtcggtgccg gcctgatcgc acgccttggg acaagcgtcg cggtgcatgt gaagggcgac
      781 tacggcttcg acgccgacgg gccgcgcagc cgacgctacg gcggcacagt cggcctcacg
      841 atcagttggt agcggtcatg cgggcagcag cgccttgagc ggggtttcgg tgtcggcgag
      901 ttgttcggca gtcgcgcgaa gcccggcggt aacgatcatc cggccctgga catagtcctt
      961 ggtcgcgttg acgcagtcga tcgcgatgac cttgcccgcc ttgaggtaga ccaccgagaa
     1021 gctccggttc gccgggtcgc cgcgcagcac cgcctgatcg tgcccggtcg agaggcccgc
     1081 ggtctggagc ttgagatcat actggttcga ccagaaccac gggatcgcat gatagggcgc
     1141 ttcatcgcca gcgatgccct tggcgacgac attcgcctgg tcgttggcgt tctgcaccga
     1201 ttcgagccgg atctcggcgc cctcggcaaa gtcgttcgcg tgcgatgcgc agtcgccgat
     1261 cgcatagatg tcggcgaggc tcgtcttgca gagcgggtcg acgcgcaccc cgttggcgca
     1321 atcggcaccc gccgccgtga gcggctcgat cgccggcacg atgccgatgc cgacgatgac
     1381 gagatcggcg ggaatgacct cgccgtcggc gagccgcacc ccggtgacat gatcgtcgcc
     1441 ctcgatcgca tcgacgcaga cgccgagccg caggtcgacg ccgtgctcgc gatgctcttt
     1501 ttcgtagaag cgcgacaggt ccgcacccgc gacgcgcgcc agcacgcggt cgagcgcttc
     1561 caggagcacg accttcttgc ccgccttgtt cagcaccgcc gccgcctcga gcccgatata
     1621 gccgccgccg atcacgacga tctgcttcgc gctttccgac gccgccttca tcgcatcggc
     1681 atcggcgcgc gtgcgcacgc cctgcacccc gggcaaatct cccccgggga tcggcagcat
     1741 gcgcggggcg ccgccggtcg cccagacgag cttgccgtaa ccgatcgtct cgccgccgtc
     1801 ggtggtgacc gtgtggccgg ccggatcgac cgacacgacg cgccggccga gcagcatcgt
     1861 cacttcgcgc tcgtcccaat atttggcggg gcgcagctgg atgcgctcga attctttctc
     1921 gcccgcgaaa tattccttcg acaagggcgg gcgttcgtag ggcagctcgg gctcgtcgcc
     1981 tatgatcgcg atgctgcctg cgaacttctg cgtccgcagc atcaccgcaa cctgcgcccc
     2041 cccgtgaccc gctcccacta tcaccacgtc gaattgcatc gctcgcgtct cccgctgctc
     2101 gtcagcgcgg cccttgcacg cgcggcggag cgaggcaagc cgcgctcgcc tcgcccatgc
     2161 gaaagaaaaa gttgaagctc agcctaccag cgtcagcgcc ggcttcgccc ggtcggcggc
     2221 gaagaccgcc gtttcgattt cctcgacttc ggatcgcagg accgcaacga cctcgcccag
     2281 cttggccgcg atgatcgcgg cctgcgccgt ctccagcccg gctaccacga cctcgatgcg
     2341 catgtgcgaa ggcaggagat gcatcgtctt cgcgctcggc acgaaagcgc gctgcgcgaa
     2401 gaaattggcg acacgcggca gcgattgcgg gtcgaggatt gcgtcgatcc ggaaacggtg
     2461 catcacgccg cctcgcgctg cgcgccggcg atgacctcct cggccatttc gtcggcgacg
     2521 atcgcgggcg agcgcccttc gcgcgcggcc cgcgcgagca gatccccgac ccgcggcgcg
     2581 atctgggcaa cgcgcagttc gacatcgctt tcgtcctcgc ccagatattc ggccgacacg
     2641 ttgatgatgc cgcccgcgtt gacgacataa tcgggcgcat aggcgatccc gcgatcgagc
     2701 agcagccccg ccacgtcggg tgtcgcgagc tggttgttgg cggcaccgca gacgagcttc
     2761 gccttcaatt tcgcgaccgt gtcgcggtcg agcgcgccgc cgagtgcgca gggcgcgaag
     2821 atgtcggcgt ccaccgatgc gatctccgac acgtcgacga cttcggcgcc atgcaccgcc
     2881 gcgagccggt cgcggcgcac cgggctgacg tc
//
LOCUS       DATMSU010000335         4170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_166764,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000335 DATMSU010000000
VERSION     DATMSU010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166764"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VN034_09110"
     CDS             <1..333
                     /locus_tag="VN034_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ionic transporter y4hA"
                     /protein_id="HWV60800.1"
                     /translation="VLGVLIAALILAPEGLAAVRAAKADRLQTSLNLALGSALATIGL
                     TIPAVAILSIVADMPISLGLDAKSTVLLFLSLIVSVQTLANGRTTVLQGVIHLMLFAI
                     YLFTTFVP"
     gene            complement(471..1607)
                     /locus_tag="VN034_09115"
     CDS             complement(471..1607)
                     /locus_tag="VN034_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWV60801.1"
                     /translation="MPQAPQPVLSATGLFTPAEAISNAELVASFNEYVALHNRENAAA
                     IAAGDVPELTESSVEFIEKASGIGSRFVVDKAGILDPAHMAPRIAERSNDEISILAEI
                     GVAAAKDALARAGRDAADVDAVLCAASNMQRPYPAMAVEIQDALGIDGFGFDMNVACS
                     SATFGIQTAADYIRAGHAKSVLVVNPEICSGHLNWRDRDSHFIFGDVATAILVEDKDI
                     APAGHWDILGTKLKTVFSNNIRNNFGFLNRGHGGIDQSGPHSDKLFVQEGRKVFKEVV
                     PMVANMIVEQMEALGLEGGDMRRLWLHQANTNMNRLIAQRVLGHEASEDESPTVLDTY
                     GNTSSAGSIIAFHLNHADMVAGDTGLICSFGAGYSAGTVFVRRT"
     gene            1798..2349
                     /locus_tag="VN034_09120"
     CDS             1798..2349
                     /locus_tag="VN034_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60802.1"
                     /translation="MATRSGPAAGDLSLSEIKEFAGFPAATQRYIRRSLDIGLERDDA
                     IARWSRDMVEETAIRVQARVYQRLHEVRGHIPDDSGLDALEHFMAPLIAVSAFDLGQD
                     RLHAFAPYRFLYERLLGAHARPWLPGAFCAAASLPHLHPDKRKLLLQSISEAAATAPG
                     WSAREPSFYPEWVDKIDETRPAN"
     gene            complement(2385..3152)
                     /locus_tag="VN034_09125"
     CDS             complement(2385..3152)
                     /locus_tag="VN034_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWV60803.1"
                     /translation="MIDLAAIDFTALLPFILIGFAAQLVDGALGMAFGVICNTLLVAV
                     LGVPPATASARIHVVEIFTTGASGLSHLFHRNIDWPLFFRLLIPGVIGGVLGAYVLSS
                     LHADVVKPFVLGYLVLIGIWLLIRGLLYPPKIAKPKVVAPLAVVGGFLDAAGGGGWGP
                     VVTSNLLVQGGEPRKVVGTVNSVEFFLAVAVSIAFIANLGINEILGPTLGLIIGGVAA
                     APLGAMMAKRFSPKVMLVMVGVVLTATSAFGLYRAVI"
     gene            complement(3277..>4170)
                     /locus_tag="VN034_09130"
     CDS             complement(3277..>4170)
                     /locus_tag="VN034_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV60804.1"
                     /translation="LDGIEAFLRVAERRSFSAAAADLGVSPSAISQTVKALEARVGAP
                     LFMRTTRSVGLTQAGEMFLERAAPAYSGLADAYEAARNLGNRPAGRLRINLMRGAVQP
                     LFEPIIAGFCETYPEIELEIYADDALADLSAGGFDAGVRMGEALDADMIAVRLTGSFR
                     FVVAAAPAYLDKYGRPETPEDLRDHRCVRFRLASGALMPWTFERGNRDFDVAVAGPII
                     VNDWIAALVAMRAGVAMGMMAEPMATAMVESGELELVLTDHAASTSGLFLYYPGRKQV
                     MPKLRAFIDYVRDNLPDDLTG"
BASE COUNT          751 a         1379 c         1332 g          708 t
ORIGIN      
        1 gtgctcggcg tgctgatcgc cgcgctgatc cttgcccccg aagggctcgc ggcggtgcgc
       61 gcggcgaagg ccgaccgcct ccagaccagc ctcaacctcg cgctcggctc ggcgctcgcg
      121 acgatcggcc tcaccatccc ggcggtcgcg atcctctcga tcgtcgccga catgccgatc
      181 agcctcggcc tcgacgccaa atcgaccgtg ctcctcttcc tgtcgctgat cgtgtcggtg
      241 cagacgctcg cgaacggccg caccaccgtg ttgcaaggcg tgatccacct gatgctgttc
      301 gcgatctacc tgttcacgac cttcgtgccg tagaactgcc gcctcttatt ctcgtcatgc
      361 tgaacttgtt tcagcatcca tggccgaggc tctcgttcgg cgccgcgctc gaggcgagat
      421 caggccatgg accctgaaac aagttcaggg tgacgaagaa aaatggcggc tcacgtcctc
      481 cgcacgaaca ccgtccccgc cgaatacccc gccccgaacg agcagatcag cccggtatcg
      541 cccgcgacca tatccgcatg gttgaggtgg aaggcgatga tcgatcccgc gctcgacgta
      601 ttgccatagg tgtcgagcac cgtcgggctc tcgtcctcgc tcgcctcgtg gcccaagacc
      661 cgctgtgcga tcagccggtt catattggtg ttcgcctggt gcagccacag ccggcgcatg
      721 tcgccgcctt cgagccccag cgcctccatc tgctcgacga tcatgttcgc gaccatcggc
      781 acgacctcct tgaacacctt gcggccttcc tggacgaaga gcttgtcgga gtgcggcccc
      841 gactggtcga tcccgccatg cccgcgattg aggaagccga aattgttgcg gatattgttc
      901 gagaagaccg tcttcaattt ggtgccgagg atgtcccaat gccccgcggg tgcgatgtcc
      961 ttgtcctcga ccaaaatcgc ggtcgcgacg tcgccgaaga tgaaatggct gtcgcggtcg
     1021 cgccagttca aatgccccga acagatttcg gggttcacga cgagcacgct tttcgcatgc
     1081 cccgcgcgaa tataatcggc ggcggtctgg atgccgaagg tcgccgacga acaggcgaca
     1141 ttcatgtcga agccaaagcc gtcgatgccg agcgcgtcct gaatctcgac cgccatcgcc
     1201 ggatagggcc gctgcatgtt cgacgcggca cagagcaccg catcgacgtc ggccgcatca
     1261 cgccccgcgc gcgccagcgc gtccttcgcc gccgcgacgc cgatctcggc gaggatcgaa
     1321 atttcgtcgt tgctccgctc ggcgatacgc ggcgccatgt gcgccggatc gagaataccc
     1381 gccttgtcga cgacgaagcg cgacccgatc ccgctcgcct tctcgatgaa ttcgacgctc
     1441 gattcggtca gctcggggac atcgcccgcc gcgatcgccg cggcattttc ccggttatgc
     1501 agcgcgacat attcgttgaa gctcgcgacc agttccgcgt tggaaatggc ttcggcgggg
     1561 gtgaagaggc cggttgcgga aaggaccggc tgcggtgctt ggggcatgat ggagctcgcg
     1621 tgtcttatgg tttcaagaca ggttgattag gcccgctcgc ggcgccgcgc aacgccctgc
     1681 acaggatgaa caggtcccga catcgtggtt aacctgccac taacgattgt cgtggcagat
     1741 tgcccattgc aggcctccgg ggggatggct ccccgggcgc agtgcgggag catgaccatg
     1801 gcgacgcgtt caggaccggc cgcaggcgac ctgtcccttt ccgagatcaa ggaatttgcg
     1861 ggctttcccg cagcgaccca gcgctacatc cgccgttcgc tcgacatcgg gctcgaacgc
     1921 gacgacgcga tcgcgcgctg gtcgcgcgat atggtcgagg aaacagcaat ccgcgtccag
     1981 gcgcgcgtct atcagcggct gcacgaagtc cgcgggcata tccccgacga cagcggcctc
     2041 gacgcgctcg aacatttcat ggcgccgctg atcgcggtgt cggcgttcga cctcgggcag
     2101 gaccggctgc atgcctttgc cccctatcgc ttcctctacg aacggctgct cggcgcgcat
     2161 gcgcggccgt ggttgcccgg cgctttctgc gccgcggcgt cgctgccgca cctgcacccc
     2221 gacaagcgca agctgctgct ccagtcgatc agcgaagccg ccgcgaccgc acccggctgg
     2281 tcggcgcgcg agccgagctt ctatcctgaa tgggtcgaca agatcgacga gacccggccg
     2341 gcgaactagg tcctgaccct ggtttccggc ggcagccgga aagatcagat cactgcgcga
     2401 tagaggccaa aggcgctggt cgcggtcagc acgacgccga ccatcacaag catcaccttc
     2461 ggcgagaagc gcttcgccat catcgcgcca agcggggccg ccgccacgcc gccgatgatc
     2521 aggccgagcg tcgggcccaa tatctcgttg atcccgaggt tcgcgatgaa agcgatcgag
     2581 accgcgacgg cgaggaagaa ttcgacgctg ttgacggtgc cgacgacctt gcgcggttcg
     2641 ccgccctgga cgagcaggtt cgaggtcacg accggacccc agccgccgcc gcccgccgcg
     2701 tcgaggaagc cgccgacgac cgccaggggc gcgacgactt tgggcttcgc gatcttgggc
     2761 ggatagagca ggccgcgaat gagcagccag atgccgatca gcaccagata tcccagcacg
     2821 aagggcttta cgacgtcggc gtgcagcgac gaaaggacat aggcgcccag gacgccgccg
     2881 atcacgcccg ggatcagaag ccggaagaac agcggccagt cgatgttgcg atggaataag
     2941 tggctgagtc ccgacgcgcc ggtcgtgaag atttcgacga catggatgcg cgccgacgcc
     3001 gttgcgggcg ggacgccgag aaccgcgacg agcaatgtgt tgcagatgac gccgaacgcc
     3061 atgcccagcg cgccgtcgac gagctgcgcc gcgaaaccga tgaggatgaa cggcaggagc
     3121 gcggtaaagt cgatggccgc aagatcaatc atatttcatc ccaacccttt tgattgacaa
     3181 cctgctgccg ggatttccgc tcgcgcgcca gcccaaattt tctgctggcg acaaagggat
     3241 ttgtttccgc gcccgatcga accgcggcaa accgggtcag ccggtcaggt catcgggcag
     3301 gttgtcgcgc acatagtcga tgaaagcgcg cagcttgggc atcacctgct tgcggccggg
     3361 ataatagagg aagagacccg atgtcgatgc ggcatggtcg gtgagcacca gttcgagctc
     3421 gcccgattcg accatcgccg tcgccatcgg ttcggccatc atgcccattg cgaccccggc
     3481 gcgcattgcg accaatgcgg cgatccagtc gttgacgata atcgggccgg cgaccgcgac
     3541 gtcgaaatcg cggttgccgc gttcaaaggt ccacggcatc agcgcgccgc tcgcaaggcg
     3601 gaaacgaacg cagcgatggt cgcgcaaatc ctccggcgtc tcggggcggc catatttgtc
     3661 gagataggcg ggcgccgcgg cgacgacgaa gcgaaaggaa ccggtcaggc gaaccgcgat
     3721 catgtcggcg tcgagcgctt cgcccatccg taccccggcg tcgaacccgc ccgcgctgag
     3781 gtcggcgagc gcgtcgtcgg catagatttc gagctcgatc tcgggatagg tttcgcaaaa
     3841 gcccgcgatg atcggctcga acaggggctg gaccgcaccg cgcatcaggt tgatgcggag
     3901 gcgccctgcg ggccggttgc cgagattgcg tgccgcctca taggcatcgg cgagccccga
     3961 ataggccggc gcggcgcgtt cgaggaacat ttcgcccgcc tgcgtaaggc ccacgctgcg
     4021 cgtcgtgcgc atgaacagag gtgcgccgac gcgcgcttcg agtgccttga cggtctggct
     4081 gatcgcggag ggcgagacgc cgaggtcggc ggcggcggcg gaaaagctgc gcctttccgc
     4141 aacacgcagg aaggcttcga tcccgtcgag
//
LOCUS       DATMSU010000336         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_185871,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000336 DATMSU010000000
VERSION     DATMSU010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185871"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="tsaD"
                     /locus_tag="VN034_09135"
     CDS             complement(<1..587)
                     /gene="tsaD"
                     /locus_tag="VN034_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWV60805.1"
                     /translation="MTLILGLESSCDETAAALVDSDRRILAHRVAGQEAEHGPYGGVV
                     PEIAARAHVDRLAPIVEGVLADAGVTLADVDAIAATAGPGLIGGVMVGLVTGKALAHA
                     AGKPLIAVNHLEGHALSPRLADPDLEFPYLLLLVSGGHCQLLLVKGVGDYRRLATTID
                     DAAGEAFDKTAKLLGLGYPGGPAVERIAAGGDPRAV"
     gene            729..1676
                     /gene="hemC"
                     /locus_tag="VN034_09140"
     CDS             729..1676
                     /gene="hemC"
                     /locus_tag="VN034_09140"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWV60806.1"
                     /translation="MNALPTTENPLRIGTRASPLAMAQAHMASAALIASHGLDPAALE
                     IVPMTATGDRIQDRALAEVGGKALWTRELDAALDTGTIDIAVHSLKDVETIRDPRFAL
                     GAMLERADPRDRLVVREGIAAATITDLPHGARLGTSSPRRAAQVKRIRPDLETVLLRG
                     NVATRLAKLAAGDADATLLAAAGLERLGMHEVGMVQAVDILLPAASQGAIGIECRAGD
                     AAVSALLARIDHAPTHLAVAAERAFLAALGGDCRSPVAAHAHWLDDGALRLDAEIFSE
                     DGADHAAGHVIVSDPGAGEALAHRLLREAPDAVRRLFAV"
BASE COUNT          258 a          574 c          591 g          273 t
ORIGIN      
        1 accgcccgcg gatcgccgcc cgcggcaatc cgttcgaccg cggggccgcc gggatagcca
       61 aggccgagca gtttcgcggt cttgtcgaac gcctcgcccg cggcatcgtc gatcgtcgtc
      121 gcgaggcggc gatagtcgcc gacacctttc acgagcagca actggcaatg gccgcccgaa
      181 acgagcagca gcagataggg gaattccaga tcgggatcgg caaggcgcgg cgacagcgca
      241 tggccttcga ggtggttgac tgcgatcagc ggcttgcctg ccgcgtgcgc gagcgctttc
      301 ccggtgacga gcccgaccat cacgccgccg atcagcccgg gcccggcggt cgcggcgatc
      361 gcgtcgacat cggccagcgt gacaccggcg tcggcgagga cgccttcgac gatcggcgcg
      421 aggcggtcga catgcgcgcg cgccgcgatt tcggggacga cgccgccata gggaccgtgt
      481 tcggcttcct gccctgcgac gcgatgcgcc aggatgcgtc ggtcgctgtc gacaagcgcc
      541 gctgcggttt cgtcgcagct cgattcaagg ccgaggatca gggtcatatg attgcgcgtc
      601 ctttacccgc ccctttgtcg gcagggcaag ccgcggtgga gccttaacag ggcttccgat
      661 acatcgggcg catttccttt cgaaaaaccg gttcccactt tttcgggaaa tgctttagca
      721 gcgacgtgat gaatgctctt ccgaccacag aaaacccgct gcgcatcggc acccgcgcgt
      781 cgccgctcgc gatggctcag gcccatatgg cgtcggcggc gctgattgcc agccacggcc
      841 tcgatcccgc ggcgctcgaa atcgtcccga tgaccgcgac cggcgaccgg atccaggatc
      901 gcgcgctcgc cgaagtcggc ggcaaggcgc tgtggacgcg cgaactcgac gccgcgctcg
      961 acacgggaac gatcgacatc gcggtccatt cgctgaagga cgtcgaaacg atccgcgatc
     1021 cgcgctttgc gctcggcgcg atgctcgagc gtgccgaccc gcgcgaccgg ctggtggtgc
     1081 gcgaggggat tgcggcggcg acgatcaccg acctgccgca cggcgcgcgg ctggggacga
     1141 gcagtccgcg ccgcgcggcg caggtgaagc ggatccggcc cgatctcgaa acagtgctgc
     1201 tgcgcggcaa tgtcgcgacg cgtcttgcca agcttgcggc gggcgatgcc gatgccacct
     1261 tgctggcagc ggcggggctc gagcggctcg gaatgcacga agtggggatg gtgcaggcgg
     1321 tggacatcct gcttcccgcc gcgtcgcaag gcgccatcgg catcgaatgc cgcgccggcg
     1381 atgcggccgt atcggcgctt ctggcccgaa tcgatcatgc gccgacccat ctggccgtcg
     1441 ccgcggaacg cgcctttttg gccgcgctgg gcggcgattg ccgctcgccc gtcgcggcgc
     1501 atgcgcattg gctcgacgat ggcgcgctgc gccttgatgc cgagatattt tccgaagatg
     1561 gcgccgatca tgcggcgggg catgtcattg tttcggatcc gggggcgggc gaagcgctcg
     1621 cgcaccggct cctgcgcgaa gcgcccgacg ccgtccggcg ccttttcgcg gtatgaccga
     1681 aggcgtcccg ctgatc
//
LOCUS       DATMSU010000337         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_34333, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000337 DATMSU010000000
VERSION     DATMSU010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34333"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VN034_09145"
     CDS             complement(<1..535)
                     /locus_tag="VN034_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60807.1"
                     /translation="MRKLAGFVATFSLGMAAVVAAPALAQDAAAATVDLSVGTKVFDS
                     EGTELGTVSSAQGANVVVDLGEGKQVTLPASSFGQLEKGPTIGATRAQVVAAVDQAAA
                     GNEAKLAAALQPGTDVRGVNGDAILGKVKLVAADGVVLTTATGDVKLPRNAFFIGQAG
                     LATSFTAEQFAAAMKQVN"
     gene            complement(773..2947)
                     /gene="lptD"
                     /locus_tag="VN034_09150"
     CDS             complement(773..2947)
                     /gene="lptD"
                     /locus_tag="VN034_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWV60808.1"
                     /translation="MAGEPDLQTPDVVPTTSDAPDTGSEQQIGFAADNLNYDGDTEVV
                     VAEGNVQMNREAIEMRADKVTWNRQTGQVFAEGNVMIKNPEGDIAYGDKIELTDSLRD
                     GVVENLLVVLDNGSRLAAVKGTRFDNGNIELENAAYTPCPVEDDEGCPKNPSWQVRAV
                     RVLYDRAKNKVRYKGARIEIFGLPLIPLPGLSHSINNEPSSGILVPEIRLDRSNGFEI
                     AVPYYLRIAPDRDMTITPHVYTDAAPMLEGEFRALTDIGSFRINGYATYGSLVPLVGE
                     APDTQKRFRGYLESAGKFQFDPRWSLTYSGRVATDRTFMRRYDISRDDRLRSTFELER
                     IGGNSYLSIAGWATQTLRVNDVQGQQPIALPIIDYRQRLDDPLFGGQLELQLNTLAIG
                     RTAGQDTQRAFAGAQWNLRGLTGLGQEVTLTALVRGDVYHSDENLLTAIPGYRGKSGW
                     QARAIAAVAADMRWPFIGEFAGGTQTLTPRVQVVATPPIKNLDIPNEDSRAFDLEDSN
                     LFAINRFNGHDRFEDGARITYGLEWNYSRPGFNINSIVGQSYRLTDKPSLFPDGTGLT
                     DRSSDIVGRTTIAYRDFLRLTHRYRLDKDNLAVRRNEFDATIGSRSTYAVVGYSRLNR
                     DILLLGEDLQDREEVRAGGRVAFAKHWSLFGSAIVDLTQQSDDPLSSADGFEPIRHRL
                     GLAYDDECLSIALTWRRDYADTGDARRGNSFSFRIAFRNLGF"
BASE COUNT          494 a          917 c         1033 g          553 t
ORIGIN      
        1 tgttcacctg cttcatcgcg gcagcgaact gttcggccgt gaagctggtc gcgagaccgg
       61 cttggccgat gaagaaggcg ttgcgcggca gtttcacgtc gccggtcgct gtggtgagaa
      121 ccacgccatc ggcggcgacg agcttgacct tgccgaggat ggcgtcgcca ttgacgccgc
      181 gcacgtcggt gccgggctgg agcgcggcgg cgagcttggc ttcgttgccg gcagcggcct
      241 gatcgaccgc ggcgacaacc tgcgcccggg tggcgccgat cgtcgggccc ttttcaagct
      301 gaccgaagga actggcgggg agggttacct gcttgccttc gccgagatcg acgacgacat
      361 tcgcgccctg cgcgctgctg acggtgccaa gttcagtgcc ttcggaatcg aaaaccttgg
      421 tgccgaccga gaggtcgacg gtcgccgcgg cggcatcctg cgccagcgcg ggggcggcca
      481 caacggcggc catgccgaga gagaaagttg caacaaagcc tgcgagtttc cgcatcgctt
      541 ggggtctcct ttgacgaatg atgattgaaa cggatgaatc ttgaacgcgc ataagcgcgc
      601 aagggccacg ccaaggctga cgaaccgtgc aggggatggg gtgaggcgtt gctggacgtg
      661 cgttcggcag aaagctttat cccgttccgg ggctcgggcc gaagtagctt ccgtcaaggt
      721 cggcaacgga ggcttgagac ggcgagtggc gggaagcgac cgaatcccgc cgtcagaagc
      781 ccagattccg gaacgcgatc cggaacgaga agctgttgcc gcgccgcgcg tcgccggtat
      841 cggcatagtc gcgccgccag gtcagcgcga tcgacaggca ttcatcgtca taggcgaggc
      901 cgagccggtg gcgaatcggc tcgaacccgt cggcgctgct cagcggatcg tcgctctgct
      961 gcgtcagatc gacgatcgcc gatccgaaca gcgaccaatg tttggcgaag gcgacgcgcc
     1021 cgcctgcgcg cacttcctcg cgatcctgca aatcctcgcc gagcagcagg atgtcgcggt
     1081 tgagccgcga atagccgacc acggcatagg tcgagcggct gccgatcgtc gcgtcgaact
     1141 cattgcggcg gaccgcgaga ttatccttgt cgagccggta gcggtgggtg aggcgcagga
     1201 aatcgcgata cgcaatcgtc gtccggccga cgatatccga cgagcggtcg gtcagcccgg
     1261 ttccgtcggg gaacaggctg ggcttgtcgg tcaggcgata gctttgcccg acgatgctgt
     1321 tgatgttgaa ccccgggcgg ctgtaattcc attcgaggcc ataggtgatg cgcgcgccgt
     1381 cctcgaaccg gtcgtggccg ttgaagcggt tgatcgcgaa gaggttgctg tcctcgaggt
     1441 cgaaggcgcg cgaatcctcg ttcggaatgt cgagattctt gatcggcggg gtcgcgacca
     1501 cctggacgcg cggggtcagc gtctgcgtgc cgccggcgaa ttcgccgatg aagggccagc
     1561 gcatatcggc cgccaccgcg gcgatcgcgc gcgcctgcca gcccgacttg ccgcgatagc
     1621 cggggatcgc ggtcagcaga ttctcgtcgc tgtgatagac gtcgccgcgc acgagggcgg
     1681 ttagcgtgac ttcctgcccg agcccggtca gcccgcgcaa attccactgt gcgccggcaa
     1741 aggcgcgctg cgtgtcctgc cccgcggtgc ggccgatcgc gagcgtgttc agttgcagct
     1801 ccaactgccc gccaaagagc gggtcgtcga gccgctggcg gtaatcgatg atcggcaatg
     1861 cgatcggctg ctggccttga acatcgttga cgcgtagcgt ctgcgtggcc cagccggcaa
     1921 tcgagagata ggagttgccg ccgatccgct cgagctcgaa agtcgagcgc agccggtcgt
     1981 cgcggctgat gtcgtaacgg cgcatgaagg tacggtcggt cgcgacgcgg cccgaatagg
     2041 tgaggctcca gcgcggatcg aactggaact tgcccgcgct ttcgagatag ccgcgaaaac
     2101 gtttctgcgt atcgggggcc tcgccgacca gcgggacgag cgagccgtag gtcgcatagc
     2161 cgttgatgcg gaacgacccg atatcggtca gcgcgcggaa ctcgccttcc agcatcggcg
     2221 ccgcgtcggt gtagacgtgc ggcgtgatcg tcatgtcgcg gtcgggggcg atacgcagat
     2281 aataggggac cgcgatctcg aagccgttgc tgcggtcgag ccggatttcg ggcaccaata
     2341 tgccgctgct cggctcgttg ttgatcgaat ggctgaggcc gggcagcggg atcagcggca
     2401 ggccgaaaat ctcgatccgc gcgcccttgt agcgaacctt gtttttcgcc ctgtcgtaaa
     2461 gcacgcgaac ggcgcggacc tgccagctcg gattcttcgg gcaaccctcg tcgtcctcga
     2521 ccgggcaggg ggtgtaggcg gcattctcca gctcgatatt gccgttgtcg aagcgcgtgc
     2581 ccttgaccgc ggcgaggcgc gatccgttgt cgagcacgac gagcagattt tcgacgacgc
     2641 cgtcgcgcaa actgtcggtc agttcgatct tgtccccata ggcgatgtcg ccttcggggt
     2701 tcttgatcat gacattgccc tcggcgaaca cctgtcccgt ctgccggttc cacgtcacct
     2761 tgtccgcgcg catctcgatc gcttcgcggt tcatctggac attgccttcg gcgacgacga
     2821 cctcggtgtc gccgtcgtaa ttgaggttgt ccgcggcaaa gccgatctgc tgttcgctac
     2881 ccgtatcggg tgcgtcggaa gtcgtcggga cgacgtcggg ggtctggaga tcgggttcgc
     2941 cggcgatttc ctccgccggc tgggccggat tcgcctcgct ctgggccagc accgggc
//
LOCUS       DATMSU010000338         1777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_91382, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000338 DATMSU010000000
VERSION     DATMSU010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91382"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="ssb"
                     /locus_tag="VN034_09155"
     CDS             complement(<1..410)
                     /gene="ssb"
                     /locus_tag="VN034_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWV60809.1"
                     /translation="MAGSVNKVILIGNLGADPEIKSFQNGGKIANIRIATSEQWKDRM
                     SGERKERTEWHNVVINGEGLVGVVERYLKKGSKVYIEGSLRTRKWQDRDGNDRYTTEV
                     VIAGMGGTLTMLDGAPGGGGGSRGGGGDEWSGGSS"
     gene            complement(444..>1777)
                     /locus_tag="VN034_09160"
     CDS             complement(444..>1777)
                     /locus_tag="VN034_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HWV60810.1"
                     /translation="GVTVPAEANDASLHQRARTIARAAIVSETPVRRWTQRVDNVVLH
                     PVAGMIILLALMFVMFQAVYAWAGPPADALEGLVGTMQTWVVDNIPQNFLRDLVVEGL
                     LAGVGAVVVFLPQILILFLFILLLEASGYMTRAAFLMDGLMGKVGLSGRGFIPLLSSF
                     ACAVPGIMATRAIPDEKDRLTTILIAPLMTCSARLPVYALIIAAFIPNRGVGGTGVGL
                     QGLVLFGLYLAGIVGALVVAFALRRTVAKGAGAGFMMEMPRYQMPQLRDVLLGLWQRA
                     WIFLRRAGTIIAMTTVVLWLLLTFPQAPEGESQVEYSVAGRIASGLEVVVAPIGFNHD
                     IALALIPAMAAREVAVSAMATANAIDAGDDEEAMAETLGERLQGKWSLATALAFLAWF
                     VFAPQCISTIAITRRETNGWKWPMFMVGYLFLLAYAAAGITYWTAVAFGLG"
BASE COUNT          328 a          602 c          568 g          279 t
ORIGIN      
        1 gacgaaccgc cgctccactc atcaccgccg ccgccgcgcg aaccgccgcc accgccgggc
       61 gcgccgtcga gcatggtcag cgtgccgccc atgcccgcga tcaccacttc ggtcgtgtag
      121 cggtcgttgc cgtcgcggtc ctgccatttg cgggtgcgca agcttccttc gatataaacc
      181 ttcgagcctt ttttgaggta acgctcgacg acgccgacca gcccttcgcc gttgatcacg
      241 acattatgcc attcggtgcg ctccttgcgc tcgccgctca tccggtcctt ccactgttcg
      301 ctggtcgcga tgcggatatt ggcgatcttg ccgccgttct ggaacgattt gatttcggga
      361 tcggcgccga ggttgccgat caaaattacc ttgttgacgc tgccagccat ccgccgctcc
      421 gctccgcttg aagattggga ggatcagccg agtccgaagg cgacggccgt ccagtaagtg
      481 ataccagcag cggcataggc gagcaggaac aaatagccaa ccatgaacat gggccatttc
      541 cacccattgg tctcgcgccg ggtgatcgcg atcgtcgaaa tgcactgcgg cgcgaagacg
      601 aaccaggcga ggaaggcgag cgcggtcgcg aggctccact tgccctgcag ccgctccccg
      661 agcgtttcgg ccatcgcttc ctcatcgtcg cccgcgtcga tcgcgttggc ggtcgccatc
      721 gccgaaaccg cgacttcgcg cgccgccatg gcggggatca gcgcgagcgc gatgtcgtgg
      781 ttgaacccga tcggcgcgac gacgacctcg agtccgctcg cgatgcgccc ggcgacgcta
      841 tattcgacct gactctcgcc ttcgggcgcc tgggggaaag tgaggagcag ccacagcacg
      901 accgtggtca tcgcgatgat cgtgcccgcg cggcgcagga agatccaagc ccgctgccac
      961 aggccgagca ggacgtcgcg cagctgcggc atctggtagc gcggcatttc catcatgaag
     1021 cccgcgcccg cgcccttcgc taccgtgcgg cggagcgcga aggcgacgac gagcgcgccg
     1081 acgatgcccg cgagatagag gccgaacagc acgagcccct gcaagccgac gccggtgccg
     1141 ccgacgccgc gattcgggat gaaggcggcg atgatcagcg catagaccgg aagccgcgcc
     1201 gaacaggtca tcaagggcgc gatcaatatc gtcgtcagcc ggtccttttc atcgggaatc
     1261 gcacgcgtcg ccatgatgcc gggcaccgcg caggcgaagc tcgacaggag cgggatgaaa
     1321 ccgcgccccg acagcccgac cttgcccatc agcccgtcca tcagaaaggc ggcgcgcgtc
     1381 atatagcccg aggcttccaa cagcaggatg aagaggaaaa ggatcagaat ctgcggcagg
     1441 aagacgacga ccgcgccgac gcccgcaagc aggccttcca cgacgaggtc gcgcaggaaa
     1501 ttctgcggga tattgtcgac aacccacgtc tgcatcgtcc cgacgaggcc ttcgagcgcg
     1561 tcggcaggcg gcccggccca ggcatagacc gcctggaaca tcacgaacat cagcgcgagc
     1621 aggatgatca tcccggcgac ggggtgcagc acgacattgt cgacgcgctg cgtccagcgg
     1681 cgcaccgggg tttcgctgac gatcgcggcg cgcgcgatcg tgcgcgcgcg ctggtggagt
     1741 gaggcgtcat tcgcttcggc cgggacggta acgcccc
//
LOCUS       DATMSU010000339         5749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_157806,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000339 DATMSU010000000
VERSION     DATMSU010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157806"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VN034_09165"
     CDS             <1..350
                     /locus_tag="VN034_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV60811.1"
                     /translation="LGPGTLADNAITQSKLAEMVLDVDTSALLIYRAGWLRDVRGERN
                     TREAALAKLHATDSAQAVIDKAVQMFGGLGVTVGTPVEALYREVRALRIYEGASEVQK
                     VVIARQHLAQSAS"
     gene            350..754
                     /locus_tag="VN034_09170"
     CDS             350..754
                     /locus_tag="VN034_09170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWV60812.1"
                     /translation="MFRTRLPLRFAHCDPAGIGYYPRYFELCDAAIEDWTAAVLGIDR
                     REMHLERGLGLPTVSLHAEFAAPSRLGDMLDFTVSVTRAGRSSVDLAVDIACAGQPRF
                     AVRYTQVLTDLAKGSSRSWPPELLERLNKELP"
     gene            751..1158
                     /locus_tag="VN034_09175"
     CDS             751..1158
                     /locus_tag="VN034_09175"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012429996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWV60813.1"
                     /translation="MTAHQSLLPPGWKRPRGYANGVTATGRMVFTAGVVGWDAEERFV
                     APDLAGQFRQILVNTLAILAEGGAGPEHIVRMTWYVTSRDEYLASLPAIGEAYRDLIG
                     KNFPAMAVVEVVALVEAEARIEIETIAVVPDAG"
     gene            1173..2348
                     /locus_tag="VN034_09180"
     CDS             1173..2348
                     /locus_tag="VN034_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV60814.1"
                     /translation="MQKFDWADPFLLDDQLEDDERMIRDSARAYAQDRLAPRAIEAFA
                     TEHTDPEIFREMGELGLLGPTIPETYGGVGASYVAYGLVAREVERVDSGYRSMMSVQS
                     SLVMYPIHAFGSEEQKRRYLPKLASGEWIGCFGLTEPDAGSDPGGMRTRAVKIDGGYR
                     LSGTKTWISNAPIADVFVVWAKSDAHGGAIRGFILEKGMRGLSAPKIEGKLSLRASIT
                     GMVVMEEVEVSEDALLPDVEGLKGPFSCLNRARYGISWGALGAAEYCFHAARQYGLDR
                     HQFGRPLAATQLFQKKLADMQTDIALGLQASLRVGRLIDEGRFAPEMISIVKRSNVGK
                     ALDIARAARDMHGGNGISGEYQVIRHMMNLETVNTYEGTHDVHALILGRAITGIAAF"
     gene            complement(2388..3713)
                     /locus_tag="VN034_09185"
     CDS             complement(2388..3713)
                     /locus_tag="VN034_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWV60815.1"
                     /translation="MRANRLDRLFAGLAVLSSLAATPAPAQEAKRRWDELCQIRTEKL
                     DLILPQAMRENDIDMWIVASREGHDDPNAAMLGGGYVGDVGYYVFTDRGGERIERVAM
                     GIGGAAFDQCPLYDIEASPAELQRLVAERAPKRIGINVATEIGTADGLSHSLHEKLRE
                     TLGPEFASRLVSAEKLVSDFRSRHTAAEIAAFARAGDYSRTIAERALSNEVIRPGQTT
                     TGEVAWWMMEELHKEGLGNSFGIPSIYVLGPGERGPTSGNHVIRPGDLITMDWGVNYL
                     LSYTDMKRMAYVLKPGETAPPQGVQRAFDRAAEIRGMILDVVRPGVTAGDALAAVNRR
                     VAETPGLVLGRYDDPSADPTVSDVVIGSHSVGDWGHGSGPSMADFNPLRMTYTLRPGN
                     FLSIELFLYTPVPEWGGRKIKIPLEDNGVVTERGFEWTAPPASRILLVK"
     gene            4142..4615
                     /locus_tag="VN034_09190"
     CDS             4142..4615
                     /locus_tag="VN034_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWV60816.1"
                     /translation="MENRGDYDPSSPTFRLENSPFYLMAHADFKYHEDMDKVLHKHGV
                     SKPIYRVMTVLREHQPASIGAIAEAALTKRSTISRIIDRMIEQGLVSTEPNPEDNRIT
                     EVTLTAAGQQTLRKLTPIVGRQFTRAMDGVSNRDIAHLLRTLQKISANLSKLPIE"
     gene            complement(4626..>5749)
                     /locus_tag="VN034_09195"
     CDS             complement(4626..>5749)
                     /locus_tag="VN034_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV60817.1"
                     /translation="AGAAGATPVPAGAARTIDATGLYIMPGLIDLHIHFTQQRCHDFG
                     KYSDSPAGAAIRGVALAEQLIGAGITAARDVGTVDDVALRIKEAVDRGIIRGPRVLWS
                     GRIIATTGGHGDEVTSTATGRQKPALGGPSKGFNGPWEWRTAVRQQIRGLADWIKLGA
                     PADKEELAAAIDEAHSLGVPVAIDSYGKYTDWAIEAGVDTLEHPLLMSDQAVPLMKKH
                     GTAFVPTIGAFDNLLTGGYPTAGIPTGGFYHTHSRRFVIDQQDHLKRVAEAYRAGVPI
                     GVGTDIPFENDERYPDAYFRELDYLSEAGMSNADVLASATRVGAEIMRMDDKLGTVEK
                     GKIADLLILGANPLDSLKNLRDVRYVVADGKIVVDGPAR"
BASE COUNT         1054 a         1938 c         1782 g          975 t
ORIGIN      
        1 agctcggccc cggcacgctc gccgacaatg ccatcaccca gtcgaagctc gccgaaatgg
       61 tcctcgacgt cgacacctcg gcgctgctga tctaccgcgc cggctggctg cgcgacgtgc
      121 gcggcgagcg caacacgcgc gaagcggcgc tggcaaaact ccacgcgacc gacagcgcgc
      181 aggcggtgat cgacaaggcg gtgcagatgt tcggcgggct cggcgtcacc gtcggcacgc
      241 ccgtcgaggc gctctatcgc gaagtgcgcg cgctgcgcat ctacgagggc gcgtcggaag
      301 tgcagaaggt cgttatcgcc cgccagcacc tcgcccagag cgcatcctga tgttcaggac
      361 ccgcctgccg ctgcgcttcg cgcactgcga ccccgcgggc atcggctatt atccgcgtta
      421 tttcgagctg tgcgatgcgg cgatcgagga ctggaccgcg gcggtgctcg gcatcgaccg
      481 gcgcgagatg cacctcgaac gcgggctcgg cctaccgacg gtcagcctgc acgccgaatt
      541 cgcggcgccg agccggctgg gcgacatgct cgactttacc gtttcagtga cgcgggcagg
      601 ccgcagctcg gtcgatctcg cggtcgacat cgcctgcgcc ggccagccgc gcttcgccgt
      661 gcgctacacg caggtgctca ccgatctggc caaaggaagc tcgcggtcct ggccccccga
      721 acttctcgaa cgactcaaca aggaactgcc atgactgctc accaaagcct tctccccccc
      781 ggctggaagc gcccgcgcgg ctatgccaat ggcgtcacag cgaccggccg catggtcttc
      841 accgccggcg tcgtcggctg ggatgcagag gaacgcttcg tcgcgcccga cctcgccggc
      901 cagttccgcc aaatcctcgt gaacacgctc gcgatcctcg ccgagggcgg cgcgggcccc
      961 gagcatatcg tgcgcatgac ctggtatgtg acgagccgcg acgaatatct cgccagcctg
     1021 cccgcgatcg gcgaagccta tcgcgatctg atcggcaaga atttccctgc gatggcggtg
     1081 gtcgaagtgg tcgcgctggt cgaggccgaa gccaggatcg agatcgagac gatcgcggtc
     1141 gtccccgacg ccggttgagc gaggatcgag acatgcagaa attcgactgg gccgacccct
     1201 tcctcctcga cgatcagttg gaggatgacg agcggatgat ccgcgacagc gcgcgcgcct
     1261 atgctcagga ccggctggcg ccgcgcgcga tcgaggcgtt cgcgaccgag cacaccgatc
     1321 ccgaaatctt tcgcgaaatg ggcgagctcg gactgctcgg cccgacgatc cccgaaacct
     1381 atggcggggt cggcgcctcc tatgtcgctt atggcctcgt cgcgcgcgag gtcgagcgcg
     1441 tcgactcggg ctatcgctcg atgatgagcg tgcagtcgag cctcgtcatg tatccgatcc
     1501 acgccttcgg ttccgaggag cagaagcggc gctatctgcc gaagctggcg agcggcgagt
     1561 ggatcggctg tttcgggctg accgaacccg acgccggcag cgacccgggc gggatgcgga
     1621 cgcgcgcggt caagatcgac ggcggctatc gcctgtcggg taccaagacc tggatttcga
     1681 acgcaccgat tgccgatgtt ttcgtcgtct gggccaaatc cgacgcgcac ggcggtgcga
     1741 tccgcggctt catactcgaa aaggggatgc gggggctgtc ggcgcccaag atcgaaggca
     1801 agctcagcct tcgcgcctcg atcaccggta tggtggtgat ggaggaggtc gaggtttccg
     1861 aagacgcgtt gctccccgac gtcgaggggc tcaaaggtcc cttctcgtgc ctcaaccgcg
     1921 cgcgctacgg gatcagttgg ggcgcgctcg gcgctgccga atattgcttc cacgccgcgc
     1981 gccaatatgg gctcgaccgg caccagttcg gccggccgct cgccgcgacg cagctgttcc
     2041 agaaaaagct cgccgacatg caaaccgaca tcgcgctcgg acttcaggcc agcctgcgcg
     2101 tcgggcggct gatcgacgaa ggccgtttcg cgcccgaaat gatctccatc gtcaagcgca
     2161 gcaatgtcgg caaggcgctc gacatcgcac gcgcggcgcg cgacatgcac ggcggcaacg
     2221 ggatcagcgg cgaatatcag gtcatccgcc acatgatgaa cctcgaaacc gtcaacacct
     2281 acgaaggcac gcacgatgtg cacgcgctga tcctcggccg cgccataacc ggcatagcgg
     2341 ccttttgatc gcgcgcgcgg gcgccccctc ccccgctgcg gcgcccgcta tttcaccagc
     2401 aagatccggc tcgcgggagg agcggtccac tcgaaaccgc gctcggtgac gacgccatta
     2461 tcctcgagcg ggattttaat cttgcgcccg ccccattcgg gaacgggggt ataaagaaac
     2521 agctcgatcg acaggaaatt gccggggcga agcgtatagg tcatgcgcag cgggttgaaa
     2581 tcggccatcg acggccccga cccgtgcccc cagtcgccga ccgaatggct gccgatcacg
     2641 acatccgaca ccgtgggatc ggcgctcgga tcatcatagc ggccgagcac aaggcccggc
     2701 gtttccgcga cgcggcggtt caccgccgcc agcgcatcgc ccgcggtgac gccgggacgc
     2761 accacgtcga gaatcatccc ccggatttcg gccgccctgt caaaggcgcg ctgcaccccc
     2821 tgcggcggcg cagtttcgcc cggcttcagc acataggcca tgcgcttcat gtcggtatag
     2881 gaaagcaggt agttcacgcc ccagtccatc gtgatcaggt cgcccggccg gatgacatgg
     2941 ttgcccgagg tcgggccgcg ttcgccggga ccgagcacat agatgctcgg aataccgaag
     3001 ctgtttccca gcccttcctt gtgcaattct tccatcatcc accaggcgac ctcgccggtg
     3061 gtggtctggc cgggcctgat cacctcgttc gacagcgcac gctcggcaat cgtccgtgaa
     3121 taatcgcccg cgcgggcaaa ggcggcgatt tcagccgcag tgtgccgcga ccggaaatcg
     3181 gacaccagct tttccgccga caccagccgc gacgcgaatt cgggccccag cgtctcgcgc
     3241 aatttctcgt gcaggctgtg gctgaggccg tctgccgtac cgatctcggt cgcgacgttg
     3301 ataccgatgc gtttcggcgc ccgttccgcg acaaggcgct gcaactctgc cggcgaagcc
     3361 tctatatcgt atagcgggca ttgatcgaag gccgcgccgc cgatccccat cgccacgcgc
     3421 tcgatccgtt cgccgccgcg atcggtgaag acataatagc cgacatcgcc gacatagccg
     3481 cccccgagca tcgcggcgtt gggatcgtca tgtccctcgc gcgaagcgac gatccacatg
     3541 tcgatgtcat tttcgcgcat cgcctgcggg aggataagat cgagcttttc cgtccgtatc
     3601 tggcacagct catcccagcg tcgctttgct tcctgtgccg gtgccggggt cgcggcgagc
     3661 gacgaaagca ccgccaggcc cgcaaacagc cgatcgagtc gattcgcccg catgttcacc
     3721 ctcctcaacc agcgcgagcc gtcacagcga ccccggcgtc cgccagcatt tgaatatatt
     3781 ttcatattca cacgaatatc gtttgattct gacgttagca gggaaggcgt gatccgcaag
     3841 gcttctgcgc gctggcgcgc cgtccgcctt ttgccgggaa gcatctgttt aggaacatca
     3901 atgccgtggc gctgggcgcg gaaatccgga ccgtcacacg aacatcactg atacggttct
     3961 atcgcccggg ccgggctttc gcacgcccga tcgccataac taagtgcaaa tacatatgtt
     4021 ttcggctaat tcgctgtcag cgccggcccg ccatgacgga acggcttgat cggacaagac
     4081 atttgaggaa cacatgaaca caaggtcgac ggggaaatcg gcaggcaggg cagaggaaaa
     4141 aatggaaaat cgtggcgact atgacccgag cagtccgacc ttccgcctgg aaaactcgcc
     4201 gttctacctg atggcgcatg ccgatttcaa atatcacgag gatatggaca aggtgctgca
     4261 caagcacggc gtgtccaaac ctatctatcg cgtcatgacg gtgctgcgcg aacatcagcc
     4321 cgcaagcatc ggcgcgatcg ccgaagccgc gctgaccaag cggtcgacga tcagccggat
     4381 catcgaccgc atgatcgaac agggcctggt ctcgaccgaa cccaatccgg aagacaaccg
     4441 gatcaccgaa gttacactga ccgccgccgg ccagcaaacg ctgcgcaagc tgaccccgat
     4501 cgtcggacgc cagttcacgc gcgcgatgga cggcgtcagc aatcgcgaca tcgcccatct
     4561 gctgcgcacg cttcaaaaga tcagcgccaa cctcagcaag cttccgatcg aatagcgccg
     4621 gcgactcaac gcgccgggcc gtcgacgacg atcttgccgt cggcgacgac atagcggacg
     4681 tcgcgaagat tcttcaacga atcgagggga ttggcgccaa ggatcaacag gtcggcgatc
     4741 ttgcctttct cgaccgtgcc gagcttgtcg tccatgcgca tgatctcggc cccgacgcgc
     4801 gtggccgatg cgaggacatc ggcgttgctc atccccgcct cgctcaaata atcgagttcg
     4861 cggaaatagg cgtcgggata gcgctcgtcg ttttcgaacg ggatatcggt gccgacgccg
     4921 atcggcaccc cggcgcgata agcttcggcg acgcgcttca gatgatcctg ctgatcgatc
     4981 acgaaacggc gcgaatgggt atgataaaag ccgccggtgg ggatccccgc ggtcggatag
     5041 ccgccggtca gcagattgtc gaaggccccg atggtgggca cgaaggcggt gccatgcttc
     5101 ttcatcaacg gcaccgcctg gtcactcatc agcagcggat gctcgagcgt gtcgaccccc
     5161 gcctcgatcg cccagtcggt atatttgcca taactgtcga tcgcgacggg cacacccagc
     5221 gaatgcgctt cgtcgatcgc ggcagcgagt tcctccttat cggccggcgc accgagcttg
     5281 atccagtcgg cgaggccgcg gatctgctgc cgcaccgccg tgcgccattc ccacgggccg
     5341 ttgaaaccct tgctgggccc gcccagcgca ggcttctgcc ggcccgtcgc ggtcgaggtc
     5401 acttcgtcgc catggccgcc ggtcgtcgcg atgatgcgtc cgctccacag cacgcgcggc
     5461 ccgcgaatga ttccgcgatc gaccgcttcc ttgatccgca gcgcgacatc atcgaccgta
     5521 ccgacgtcgc gcgccgcggt gatgccggcg ccgatcaact gttcggccag cgcgacgccc
     5581 cggatcgcgg cacccgcggg gctgtccgaa tatttgccga agtcatggca acgctgctgc
     5641 gtgaaatgga tgtggaggtc gatcaggccc ggcatgatat aaaggccggt cgcgtcgatg
     5701 gttcgcgcgg ccccggccgg caccggggtt gcccccgccg ccccggccc
//
LOCUS       DATMSU010000340         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_53227, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000340 DATMSU010000000
VERSION     DATMSU010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53227"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VN034_09200"
     CDS             <1..918
                     /locus_tag="VN034_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWV60818.1"
                     /translation="GGTATRPAEVQLTRGGDARILTDLNRSLREVKSLGEVRKITVAS
                     SHDGKTIEGWLTLPPGYREGQRVPLILEIHGGPFTAYGPHFSTDNQLYAAGGYAVLSA
                     NPRGSTSYGEAFANEIDKAYPGNDYFDLISIVDRAIELGVADPDALFVTGGSGGGVLT
                     SWIVGKTNRFKAAVTQKPVINWTTQALTADGPGFFGPYWLGAQPWEKPELFWARSPLS
                     LVGNVETPTMVIVGAEDYRTPVSESEQYYTALRLRGVPTALVKVPGASHGSIAARPSQ
                     SAAKASAILAWFEKYRKGWTRPATDTAAE"
     gene            complement(1106..2203)
                     /gene="ychF"
                     /locus_tag="VN034_09205"
     CDS             complement(1106..2203)
                     /gene="ychF"
                     /locus_tag="VN034_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWV60819.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTETAAAQAANYPFCTIEPNIGN
                     VAVPDDRLEKLAAIASSKKIIATQLAFVDIAGLVRGASKGEGLGNQFLGNIREVDAIV
                     HVLRCFEDDDIQHVENKVDPVADAETVETELLLADLESLEKRVPAFAKKAAQGDKEAK
                     IAASVLGQALDLLRDGKPARLVEPKDDEEARVLRTAQLLTSKPVLYVCNVDEGSAANG
                     NAFSEKVFAKAAAEGAQAVVVSAAIESELVTMDMADRLEFLEEMGLHETGLARVIRAG
                     YELLHLITFFTVGPQEARAWTVHTGATAPEAAGEIHSDFQKGFIRAETIAYDDYVTLG
                     GEAKAREAGKLRAEGKAYVVSDGDVMHFLHS"
     gene            2335..2997
                     /locus_tag="VN034_09210"
     CDS             2335..2997
                     /locus_tag="VN034_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWV60820.1"
                     /translation="MTKPRHLAAGMLLAFGVTAAVAAQSRFEEGVFTELNRFRSDPAG
                     YADYLGDYRPRFEGKLLIGNDDDEIDIMTREGVAAVDEAIRDLRREKPLPTLAWSDPL
                     ARAAADHVAVQSRSGAVGHYTRGSGPGERMKARGGGPYVSEVITYGHHTPEGVVDQLL
                     IDDGVPDRGHRHSLLRPAHRYAGIACGRHPVHRTMCVTLMSQTRDGSPPPPPRRAATK
                     AE"
BASE COUNT          475 a         1008 c          999 g          530 t
ORIGIN      
        1 ggcggcaccg cgacgcgccc cgccgaggtg cagctgacgc gcggcggcga cgcgcgcatc
       61 ctcaccgacc tcaaccgctc gcttcgcgaa gtgaaatcgc tcggtgaggt ccgcaagatc
      121 accgtcgcct ccagccacga cggcaagaca atcgagggct ggctcaccct cccgcccggc
      181 taccgcgagg ggcagcgcgt cccgctgatc ctcgaaatcc acggcggccc tttcaccgcc
      241 tatggcccgc atttctcgac cgacaaccag ctctacgccg cgggcggtta cgccgtgctc
      301 tcggccaatc cgcgcggttc gaccagctat ggcgaggctt tcgcgaacga gatcgacaag
      361 gcctatccgg gcaatgatta tttcgacctc atcagcatcg tcgaccgtgc gatagagctt
      421 ggcgtcgccg atcccgatgc gctgttcgtc accggcggtt cgggcggcgg cgtgctgacg
      481 agctggatcg tcggcaagac gaaccggttc aaggcggcgg tcacgcaaaa gccggtgatc
      541 aactggacga cgcaggcgct caccgccgac ggtcccggct tcttcggccc ctattggctc
      601 ggcgcgcagc cgtgggaaaa gcccgaactg ttctgggcgc gctcgccgct ttcgctcgtc
      661 ggcaatgtcg aaaccccgac gatggtgatc gtcggcgccg aggattaccg cacgccggtc
      721 agcgaatccg agcaatatta caccgcgctc cgcctccgcg gcgtgcccac cgcgctggtc
      781 aaggtacccg gcgccagcca cggcagcatc gccgcgcgcc cctcgcaatc ggccgccaag
      841 gcatcggcga tcctcgcctg gttcgaaaaa taccggaaag gctggacgcg cccggcgacg
      901 gacacggcgg cggaatagcc aggccgagcc ggggcagctt aaaactccgc tttcctatat
      961 tgttacgatg tgccggcggg cgacggactt gttgatatgt tggcaatccg gtcctctggc
     1021 ggaggcaaca ggggcgacat tttagacgcg cccgcgctga gcctttgcgg ccttaagccg
     1081 accggtttgg cgtcaagacg gtgcttcagc tgtgcaggaa gtgcatgacg tcgccgtcgc
     1141 tcacgacata ggccttgcct tcggcgcgga gcttgcccgc ttcgcgcgcc ttcgcttcgc
     1201 cgccgagcgt gacataatcg tcataggcga tcgtttcggc gcggatgaag cccttctgga
     1261 aatcgctgtg gatctcgccc gcggcttcgg gcgcggtcgc acccgtgtgg acggtccagg
     1321 cgcgcgcttc ctgcgggccg acggtgaaga aggtgatgag gtggagcaac tcgtaaccgg
     1381 cgcggatgac gcgcgcgagc ccggtttcgt ggaggcccat ttcctcgagg aattcgagcc
     1441 ggtcggccat gtccatcgtc accagctcgc tctcgatcgc ggccgagacg acgacggctt
     1501 gggcgccctc ggccgccgcc ttggcgaaga ccttttccga aaaggcgttg ccgttcgcgg
     1561 cgctgccctc gtcgacgttg cagacataga ggacgggctt cgaggtcagc agctgtgcgg
     1621 tgcgcagcac gcgggcttcc tcgtcgtcct tcggctcgac gagccgcgcg ggcttgccgt
     1681 cgcggagcag gtcgagcgcc tgtccgagca ccgatgcggc gatcttcgct tccttgtcgc
     1741 cctgcgccgc tttcttggcg aaggcgggca cgcgcttttc gaggctttcg aggtcggcga
     1801 gcagcagctc ggtttcgacc gtttcggcgt cggcgacggg gtcgaccttg ttctcgacat
     1861 gctggatgtc gtcatcctcg aagcagcgca ggacgtggac gatcgcatcg acttcgcgga
     1921 tattgccgag gaactggttg ccgagccctt cgcccttcga ggcgccgcgc acgaggccgg
     1981 cgatgtcgac gaaggcgagc tgggtcgcga tgatcttctt gctgctcgcg atcgcggcga
     2041 gtttctccag ccggtcgtcg ggcaccgcga cattgccgat attcggctcg atcgtgcaga
     2101 agggatagtt cgccgcctgc gccgccgcgg tttcggtcag cgcgttgaac agggtggact
     2161 tgccgacgtt gggcaggccg acgatcccgc atttgaaacc cataaaaact ccgtgaggtc
     2221 cggcatgccg tgaatgaggg gccggtgttg ctgcgccccc tagcgccaag tcgcgcccga
     2281 tgccagtctt tgccgttgcg cgccgttcag acttggcgct ttatcgcgcg agccatgacg
     2341 aaaccccgcc atctcgccgc cggcatgctt ctggcctttg gcgtgaccgc cgcggtggcc
     2401 gcgcagagcc ggttcgagga aggcgtcttc accgaactca accgcttccg cagcgatcca
     2461 gccggctacg ccgactatct cggcgattat cgcccgcgtt tcgagggcaa gctgctgatc
     2521 ggcaatgatg atgacgagat cgacatcatg acgcgcgagg gcgtcgcggc ggtcgacgag
     2581 gcgatccgcg accttcgccg cgaaaaaccg cttccgacgc tcgcatggag cgatccgctc
     2641 gcgcgcgctg cggcggacca tgtcgcggtc cagtcgcgct cgggcgcggt cggccattat
     2701 acgcggggta gcgggcccgg cgaacggatg aaggcgcgcg gtggcggtcc ctatgtcagc
     2761 gaggtcatca cctatggcca tcacacgccc gaaggcgtcg tcgatcagct gctgatcgac
     2821 gatggcgtgc ccgatcgcgg ccatcgccac agcctgcttc gcccggcgca ccgctatgcc
     2881 gggatcgcct gcggccgcca tccggtgcat cggacgatgt gcgtgacttt gatgtcgcaa
     2941 acgcgcgacg gctccccgcc gcccccgccg aggcgggcgg cgacgaaggc cgaatgagag
     3001 cagcggggcc gg
//
LOCUS       DATMSU010000341         7399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_44220, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000341 DATMSU010000000
VERSION     DATMSU010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44220"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..380
                     /locus_tag="VN034_09215"
     CDS             108..380
                     /locus_tag="VN034_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60821.1"
                     /translation="MRWPMWCAASWNADARAYVGTSLWRFATREIILAEAGLAAAGWF
                     ERPFQTLDVRTGANGSREIPVTPGWLRWETIPLSGGRTGRLTTTLR"
     gene            462..1073
                     /locus_tag="VN034_09220"
     CDS             462..1073
                     /locus_tag="VN034_09220"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWV60822.1"
                     /translation="MEQTIQTDPARLEAILAFVQAAEKLKDTLRSGITAQGRAESSAE
                     HSWRLCLMTILFDRELDDHDRLKLLKLCIVHDLGEAISGDVPATLQVAGDGRAAQERA
                     DLVTLCAPLPGDLRQEIVALWDEYSDATSPEAILAKGFDKLETMLQHLVGKNGPGFDY
                     RFNLGYGAKHTARHPLLAQLRVLVDRETQKRVAMMAPAEAARS"
     gene            complement(1063..1491)
                     /locus_tag="VN034_09225"
     CDS             complement(1063..1491)
                     /locus_tag="VN034_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV60823.1"
                     /translation="METQLLYRGRLIDHIQLVVADIKASRTFYDAVFGVLGIPLGGEI
                     GDDFWFDELFVSTKDSEAALGELTGRHHLAFQAADRGAVDAFYKAGLAAGGRDKGAPG
                     ERPYHPGYYAAFLLDPDGNNIEAVYHGEHQRSAPSVEISF"
     gene            complement(1568..2050)
                     /locus_tag="VN034_09230"
     CDS             complement(1568..2050)
                     /locus_tag="VN034_09230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV60824.1"
                     /translation="MLIRPATFGDAAAIWAILEPVIRAGETYTLDRDMREADALAYWF
                     GADKEVFVAEADGAILGTYYLRANQAGGGAHVCNAGYMTSAAATGRGVARAMGLHSIE
                     HAKARGFRAMQFNFVVSSNVRAAGLWQSLGFAVVGRLPEAFAHPSEGFVDALVMFRTL
                     "
     gene            complement(2069..2752)
                     /locus_tag="VN034_09235"
     CDS             complement(2069..2752)
                     /locus_tag="VN034_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWV60825.1"
                     /translation="MDIVETKKSKPSVRKRAEAIRSFRCKNLVAVIEDPLDIRNIGTV
                     IRNVNALGVEKVYVVDPRKALPDDWQDMRERKSLSSTSVSAVKWTFVKRFDSTEACLN
                     HLEKMGFVSVVTSPHVKGRTNVYLHEGDYTQHTKLAVWFGNEKHGISELAVGRSDMCI
                     AVPMFGMIESLNLGTSSGIVLYEVAKQRREYQSQYRRGRHKGKRGDPLPTVMLRDDES
                     SIELESDIG"
     gene            complement(2802..3194)
                     /locus_tag="VN034_09240"
     CDS             complement(2802..3194)
                     /locus_tag="VN034_09240"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWV60826.1"
                     /translation="MNEFTLQLTATPDAIDELGHVNNAVWVQWIQQVATGHWEAAAPE
                     AHRQAYIWVVVRHEIDYLRALGPGETVTARTWVAERPQGAKFDRFMEFTGEGGKVHVR
                     ARTVWALLDKASGRPLRVTDAILAPFAG"
     gene            complement(3352..4602)
                     /locus_tag="VN034_09245"
     CDS             complement(3352..4602)
                     /locus_tag="VN034_09245"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWV60827.1"
                     /translation="MTDQLTLTPAADFPAITLDDVRAAAGRINGSVVRTPTLHSQTLS
                     EMVGAEVWLKFENLQFTAAYKERGALNALLLLSDEAKARGVIAASAGNHAQGLAYHGK
                     RLGVPVTIVMPKTTPQVKVSQTASHGAEIVLFGEKFDDASAHARVLEKERGLTFVHPF
                     DHPHIAAGQGTVALEMLEDVPELDTMIVPIGGGGLLTGMGTAARGMKNDMRLIGVQAE
                     LYPSMYAELNGVDMACEGDTLAEGIAVKEPGAYTRKIVAELNDDIVLVAERHLERAVS
                     LLLQIEKTVVEGAGAAGLAAMLAHPEEFAGRKVGLVLTGGNIDTRLLANVLLRDLARS
                     GRIARLRIRLQDRPGALFKVMKLFDEKQVNIIEIYHQRIFTTLPAKGLITDIECEARD
                     REHLDSLVASLRDAGYMVTTVELA"
     gene            4659..5537
                     /locus_tag="VN034_09250"
     CDS             4659..5537
                     /locus_tag="VN034_09250"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60828.1"
                     /translation="MSERKLRISFIGTGVMGGPMAGHLVKAGHNLTVYNRTRAKAEAW
                     VGQHGGTAAATPAEAAKDADVVLTCVGNDDDLAQVTLGRDGAFKAMKKGALFVDHTTV
                     SARIARQLSVEADGLGLLCVDAPVSGGEAGAQNGTLSIMCGGTKAAFDAAAPVMQAYA
                     ARMVHIGGPGAGQTTKMVNQIAIAGVVQGLSEALRFAQASKLDTDKVFEAVSGGAAAS
                     WQMLNRWGTMAQDEFDFGFAVDWMRKDLGLALDEARVNGATLPVASLVDQFYADVQKA
                     GGGRKDTSSLVTRLPK"
     gene            5702..7336
                     /locus_tag="VN034_09255"
     CDS             5702..7336
                     /locus_tag="VN034_09255"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV60829.1"
                     /translation="MALAAPAHADTLIDNVNGITLDKDGKLVRFTGLVIDTEGKVKQL
                     LDRKDKRPERPDFKQDGRGRTLIPGLIDAHGHVMGLGFKLMLLDLSETNSLAEAQAAI
                     RKYAAENPEMPWIIGTGWNQEKWGLGRFPTSADLDAAVPNRPVWLERVDGHAGWANSA
                     AMAAAKITPASKSPEGGRIEMAGGKPSGVFVDAAMSLIDSAKPKPLARDLDRALYLAQ
                     QKLLEQGITTIADMGTTIEEWQAYRRAGDKKQLAVRILSYGGDIDNMAIIAGSEPTPW
                     LYDDRLRMVGVKLYLDGALGSRGAWLKAPYADAPGQKGLPLLTPAQLRNKMVRASMDK
                     FQVAIHAIGDAANAEALAAIADLTADLPGERRWRIEHAQIIDPADIARFAELKVIASM
                     QPVHQPSDRVMAEARLGPDRLKGAYAWRSLQNAGVRLAFGSDVPVESANPFAGIAAAI
                     SRTDAKGEPFGGWRPEEAVSRETALDGFTRTAAYAGFAEDRIGTLMPGMRADFLIVDA
                     DPMLASPDEIRRMTPLETWIGGYRYYKKKEGATVGR"
BASE COUNT         1314 a         2541 c         2321 g         1223 t
ORIGIN      
        1 gaattgggtc gatcagttca acgatccgca actgcgcgcg ctggtggatg aggccatcaa
       61 ggacaacccg aacctgcaag tcgcctttgc ccgcgtgcgc gcttcgcgtg cgatggccga
      121 tgtggtgcgc ggcgtcgtgg aatgccgatg cccgtgccta tgtcggcaca tcgttgtggc
      181 gcttcgcgac gcgcgaaatc atcctcgccg aggcggggct cgcggcggcg ggctggttcg
      241 agcgcccgtt ccagactctc gacgtccgga ccggcgccaa cggtagtcgc gaaatccccg
      301 tcacccccgg atggctgcgc tgggaaacca ttccgctttc aggcggccgc accggacgtc
      361 tgacgacgac gcttcgctga cgcgcatatt ttttacgtgt ccgcgctcgg ggcgcattcc
      421 tatggcgata ttcatcaagc gtcgctgtga aggaccgccc gatggaacag accattcaga
      481 cagatcccgc tcggctcgag gccatcctcg ctttcgtcca ggctgccgaa aagctgaagg
      541 atacgctgcg cagcggcatc accgcacagg gccgcgccga gagcagcgcc gaacatagct
      601 ggcggctctg cctgatgacg atcctgttcg accgcgaatt ggacgatcac gaccgcctca
      661 aactgctcaa gctgtgtatc gtgcacgacc tcggcgaagc gatttcgggc gacgttccgg
      721 cgacgctgca ggtcgcaggc gacggacgcg ccgcgcagga acgcgccgac ctcgtgacgc
      781 tgtgcgcccc gttgccgggc gaccttcgcc aggaaatcgt cgcgctgtgg gacgaatata
      841 gcgacgccac ctcgcccgag gcgatcctgg ccaagggatt cgacaagctc gaaaccatgc
      901 tccagcacct cgtcggaaag aatggccccg gcttcgacta ccggttcaat ctaggctatg
      961 gcgcgaagca taccgcgcgc catccgctgc tcgcgcaatt gcgcgtcctc gtcgaccggg
     1021 aaacgcaaaa gcgcgtggcg atgatggcgc cggccgaggc ggctagaagc tgatctccac
     1081 cgacggcgcg ctccgctgat gctcgccatg atagaccgcc tcgatattgt tgccgtcggg
     1141 atcgagcagg aacgcggcat aatagcccgg atgatagggc cgctcgcccg gcgcgccctt
     1201 gtcgcggccg cccgcggcaa ggcccgcctt gtaaaaggca tcgacggcgc cgcgatccgc
     1261 cgcctgaaag gccaggtgat gccgcccggt caattcgccg agcgccgctt cgctgtcctt
     1321 ggtcgatacg aacaattcgt cgaaccagaa atcatccccg atctcgccgc cgagcggaat
     1381 accgagcacg ccgaagaccg catcgtagaa tgtgcggctg gccttgatgt cggcgacgac
     1441 aagctggata tgatcgatca gccggccgcg atacagcaat tgcgtttcca tcgtctctct
     1501 cccgtttctc cggaccggca gagatacggc cgcgccgttc cgaaggttcc caacgaggaa
     1561 caggaggtca gagcgtccgg aacatcacca gcgcatcgac gaagccctcg gacggatgcg
     1621 caaacgcctc tggcaaccgg ccgacgaccg cgaaccccag cgactgccac agcccggccg
     1681 cgcggacgtt gctgctgacg acgaagttga actgcatcgc gcgaaagccg cgcgccttcg
     1741 cgtgctcgat cgagtgcagg cccatcgccc gcgccacccc gcgcccggtc gccgccgcgc
     1801 tagtcatata gcccgcgttg cagacatgcg cgccgccgcc cgcctggttg gcgcgcagat
     1861 aataggtgcc gaggatggcg ccgtcggcct cggcgacgaa cacctccttg tcggccccga
     1921 accaataagc gagcgcgtcc gcctcacgca tgtcgcggtc cagcgtatag gtctcgccgg
     1981 cgcggatcac gggttcgagg atcgcccaga tcgcggctgc gtcgccgaac gtagcgggtc
     2041 ggatcagcat attgcccacc atcacgtgtc agccgatatc gctttccaac tcgatgctgc
     2101 tctcgtcatc gcgcagcatc acggtcggca gcggatcacc gcgttttcct ttgtgccggc
     2161 cacggcgata ctggctctga tattcgcgcc gctgctttgc gacctcgtaa agaacaatac
     2221 ccgaactggt cccaagattc aggctctcga tcattccgaa catcgggact gcgatgcaca
     2281 tgtcgctgcg cccgacggca agttcgctga ttccgtgttt ctcgttgccg aaccacaccg
     2341 ccagtttcgt gtgctgcgta tagtcgcctt cgtgaagata gacattcgtc cggcccttta
     2401 cgtgtggcga ggtcacgaca gacacaaatc ccattttctc gagatggttg aggcaggctt
     2461 cggtgctgtc gaaccgcttc acgaaggtcc atttgaccgc ggataccgag gtgctcgaca
     2521 gtgatttgcg ttcgcgcatg tcctgccagt catcaggcaa cgcctttcgc ggatcgacca
     2581 catagacttt ttcgacgccg agcgcgttca cgtttcggat gaccgtgccg atattcctga
     2641 tatccaacgg atcttcaatg accgcgacca gattcttaca gcggaacgac cggattgcct
     2701 ccgcacgctt acggaccgac ggtttggatt tcttggtctc gacgatgtcc atcgcgcgat
     2761 cttagcattt tggctgctgc cttcaagcga gcggctgccg tctatcccgc aaaaggcgcg
     2821 aggatcgcgt cggtcacccg caagggccgc ccgctcgcct tgtcgagcag cgcccacacc
     2881 gtccgcgccc gtacatggac cttgccaccc tcaccggtga actccataaa gcggtcgaac
     2941 ttcgcgccct gcggccgctc ggcgacccaa gtccgcgcgg tcaccgtctc gccggggccg
     3001 agcgcgcgga gatagtcgat ctcgtgccgc accacgaccc agatataggc ttgcctgtgc
     3061 gcctcaggcg ccgcggcttc ccaatggccg gtggcgacct gctggatcca ctggacccag
     3121 accgcattgt tgacatggcc gagctcgtcg atcgcatccg gggtggcagt gagttggagg
     3181 gtgaattcat tcatcggtgg accatagcaa gcccctctcc ttcaggggag gggttggggt
     3241 ggggctcgct gggtagcgca gggccgatgg ctcccacccc gctcgactaa ggcccggcta
     3301 cgccgagcca agtctcactg cccctcccct gaaggggagg ggcttgaaac gtcacgccag
     3361 ctcgaccgtc gtcaccatat accccgcgtc gcgcagcgaa gcgacgaggc tgtcgagatg
     3421 ctcgcggtcg cgcgcctcgc attcgatgtc ggtgatcagc cccttcgcgg gcagcgtcgt
     3481 gaagatgcgc tggtgataga tttcgatgat gttgacctgc ttctcgtcga acagcttcat
     3541 caccttgaac agcgcgcccg gccggtcctg caggcggatg cgcaaccgcg cgatacggcc
     3601 cgagcgcgcg aggtcgcgca gcagcacatt ggcgagcagc cgcgtgtcga tattaccgcc
     3661 ggtgagcacc agcccgacct tgcgcccggc aaattcctcg ggatgcgcga gcattgccgc
     3721 gagccccgcc gcgcccgcgc cctcgacgac ggtcttttcg atctggagca acaggctgac
     3781 cgcgcgctcg agatgccgtt cggcgacgag cacgatgtcg tcgttgagtt cggcgacgat
     3841 cttgcgcgtg taagcgcccg gttccttgac cgcgatgccc tcggcgagcg tgtcgccctc
     3901 gcacgccatg tcgacaccgt tgagttcggc atacatcgac ggataaagct cggcctgcac
     3961 cccgatcagc cgcatgtcgt tcttcatccc gcgcgccgcg gtgcccatgc cggtgagcaa
     4021 cccgccgccg ccgatcggca cgatcatcgt gtcgagttcg ggcacatcct cgagcatttc
     4081 gagcgccacc gtcccctgcc ccgcggcgat atgcgggtga tcgaacggat ggacgaaggt
     4141 gagcccgcgc tctttctcga gcacccgcgc atgcgccgaa gcgtcgtcga acttctcgcc
     4201 gaacagcacg atttcggcgc cgtggctcgc ggtctgcgac accttcacct gcggcgtcgt
     4261 cttcggcatc acgatggtga ccgggacgcc gagccgcttg ccatgatagg cgagcccctg
     4321 cgcatgattg cccgccgacg ccgcgatcac gccgcgcgcc ttggcttcgt cgctgaggag
     4381 gagcagcgcg ttgagcgccc cgcgctcctt gtaggccgcg gtgaattgca gattctcgaa
     4441 cttcagccac acttcggcgc cgaccatttc ggacagggtc tgcgaatgga gcgtcggcgt
     4501 ccgcacgacc gacccattaa ttcgccccgc cgccgcgcgc acatcgtcca atgtgatcgc
     4561 cggaaaatcg gcggcgggcg tcagggtgag ttgatcggtc ataggcgaag cgccctagcc
     4621 catctggcgc acgaagcaaa caatgctatg gactgcgcat gagcgaacga aaattgcgta
     4681 tcagcttcat cggtaccggc gtcatgggcg gaccgatggc aggccacctc gtcaaggcgg
     4741 gccataacct taccgtctat aatcgcaccc gcgccaaggc cgaagcgtgg gtcggacagc
     4801 atggcggcac cgcggcagcg accccggccg aggccgcgaa ggacgccgac gtcgtcctca
     4861 cctgcgtcgg caacgacgac gatctcgcgc aagtgacgct cgggcgcgac ggcgcgttca
     4921 aggcgatgaa gaagggcgcg ctgttcgtcg accacaccac cgtctccgcg cgcatcgcgc
     4981 gccagctttc ggtcgaggcc gacgggctcg gcctcctctg cgtcgatgcc cccgtctcgg
     5041 gcggcgaagc gggcgcgcag aacggcacgc tttcgatcat gtgcggcggc accaaggccg
     5101 cgttcgacgc cgccgcgccg gtgatgcagg cttatgcggc gcgcatggtg catatcggtg
     5161 gccccggcgc ggggcagacg acgaagatgg tcaaccagat cgcgatcgcc ggggtggtcc
     5221 agggcctctc cgaagcgctg cgtttcgcac aggcgtcgaa gctcgacacc gacaaggtgt
     5281 tcgaggccgt ctccggcggc gccgcggcga gctggcagat gctcaaccgc tggggcacga
     5341 tggcgcagga cgagttcgac ttcggcttcg cggtcgactg gatgcgcaag gacctcggcc
     5401 tcgcgctcga cgaagcgcgc gtcaacggcg cgacgctccc cgtcgccagc cttgtcgacc
     5461 aattctacgc cgatgtgcaa aaagccggcg gcggccgcaa ggacacgagt tcgctcgtga
     5521 cgaggctgcc gaaatgaggg ggcatacgaa acacgccctc cccttcaggg gagggcagcg
     5581 agacttggga gcttgttccc tagtcgcagc gggtgggggg cagcagcctt acgccgcgcc
     5641 gcgagccccc accccaaccc ctcccctgaa ggggaggggc ttaattgccg ccctcgcagc
     5701 cttggcgctc gctgcccccg cgcacgccga cacgctgatc gataacgtca acggcatcac
     5761 gctcgacaag gatggcaagc tcgtgcgctt caccggcctc gtcatcgata ccgagggcaa
     5821 ggtgaagcag ctgctcgacc gcaaggacaa gcgccccgaa cgccccgatt tcaagcagga
     5881 tggcaggggc cgcaccctca tccccggact gatcgacgcg cacggccatg tcatggggct
     5941 cggcttcaaa ctgatgctgc tcgacctctc ggaaacgaac agcctcgccg aagcgcaggc
     6001 ggcgatccgc aaatatgccg ccgaaaatcc cgaaatgccg tggatcatcg ggacggggtg
     6061 gaatcaggag aaatggggcc ttggccgctt tcccaccagt gccgatctcg acgccgcggt
     6121 gcccaaccgc cccgtttggc tcgaacgcgt cgacggccac gccggctggg cgaacagcgc
     6181 ggcgatggcg gcggcgaaga tcacccccgc gagcaagtcg cccgaaggcg gccgcatcga
     6241 gatggcgggc ggcaagccgt cgggcgtctt cgtcgacgcg gcgatgagcc tgatcgacag
     6301 cgccaagccc aaaccgctcg cgcgcgacct cgaccgcgcg ctgtatctgg cgcagcagaa
     6361 gctgctcgaa caggggatca cgacgatcgc cgacatgggc acgacgatcg aggaatggca
     6421 ggcctatcgc cgcgccggcg acaagaaaca gctcgccgtc cgtatcctca gctacggcgg
     6481 cgacatcgac aatatggcga tcatcgccgg gtccgaaccg acgccgtggc tctacgacga
     6541 ccggctgcgc atggtcgggg tgaagctcta tctcgacggc gcgctgggct cgcgcggcgc
     6601 gtggctgaaa gcgccttatg ccgacgcgcc ggggcagaag gggcttcccc tcctcacgcc
     6661 cgcgcagctt cgcaacaaga tggtgcgcgc gtcgatggac aagttccagg tcgcgatcca
     6721 cgcgatcggc gacgccgcca acgccgaagc cctcgccgcg atcgccgacc tcaccgccga
     6781 cctaccgggc gaacgccgct ggcgcatcga acatgcgcag atcatcgacc ccgccgacat
     6841 cgcacgcttt gccgagctca aggtcatcgc ctcgatgcag ccggtccacc agccgagcga
     6901 ccgcgtgatg gccgaagcgc gcctcggtcc cgaccgcctc aagggcgcct atgcgtggcg
     6961 cagcctccag aatgcgggcg tccgcctcgc cttcggctcc gacgtgccgg tcgaaagcgc
     7021 caaccccttc gcgggcatcg ccgccgccat ctcgcgtacc gatgcaaagg gcgagccctt
     7081 cggcggctgg cgccccgagg aagcggtcag ccgcgaaacc gcgctcgacg gtttcacccg
     7141 caccgccgcc tatgcgggtt tcgccgagga ccggatcggc acgctgatgc ccgggatgcg
     7201 cgccgatttc ctgatcgtcg acgccgaccc gatgctcgcg agccccgacg agatccgccg
     7261 catgacccca ctcgaaacat ggatcggcgg ctatcgatat tacaagaaga aggagggcgc
     7321 gaccgttggc cgctgacacg ccgaccaaag gcgtgccgtc gcgccgcgcg ctcctcaccg
     7381 gcctcgctgc gttgccagt
//
LOCUS       DATMSU010000342         1985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_72303, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000342 DATMSU010000000
VERSION     DATMSU010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72303"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VN034_09260"
     CDS             complement(<1..120)
                     /locus_tag="VN034_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60830.1"
                     /translation="MSHMLIFGMGYAASHLAGRLRARGWDVEGTTRDGRAGGIA"
     gene            167..>1985
                     /gene="pepN"
                     /locus_tag="VN034_09265"
     CDS             167..>1985
                     /gene="pepN"
                     /locus_tag="VN034_09265"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWV60831.1"
                     /translation="MTDLSSTPAIPVTIHRGDYSPPEWQVPDIALDFALGLEETKVAA
                     ALSVVRHADAPAPLVLRGDGLTPAAVRVDGEVWNDWRIDGSDLIVDLGERTAAMVEIE
                     TVINPAANTQLMGLYASNGMLCTQCEAEGFRRITFHPDRPDVLSRYKVRMEGDKAAFP
                     ILLSNGNCIERGEGEAGTHWALWEDPWPKPSYLFALVAGDLVVNRDSFTTMSGRKVEL
                     GIWVREGDVDRTRHAMDALKNSMKWDEAVYGREYDLDLFNIVAVSDFNMGAMENKGLN
                     IFNTRYILADPDTATDLDYDGVEGVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRD
                     QNFSADMGSPPVKRIEDVRLLRAAQFPEDAGPLAHPIRPDSYQEISNFYTATVYNKGA
                     EIIRMMHTMVGPERFRKGTDLYFERHDGEAATCEDFVRAIEDGAKVDLAQFRLWYGQA
                     GTPRITARIDTDAATGDHTLHLAQLVPPTPGQPDKAPMLIPLRIKAYDPGSGDGDEAE
                     QLILLDRAEMAVPLGPYRSRPMLSINRGFSAPVIVDFAREDGELAWLAAHDDDPFARY
                     EALQQLMLDTLVAAVSGKGGGSGAVIEAVAQTLAGRADDP"
BASE COUNT          349 a          638 c          653 g          345 t
ORIGIN      
        1 ggcgatgccg ccggcgcgcc cgtcgcgcgt cgttccctcg acgtcccatc cgcgcgcgcg
       61 cagccgtccg gcaaggtggc tcgccgcata gcccattccg aaaatcagca tatggctcat
      121 cgcgcgcctt attgcgcgtc ggcggcgtcc tgcctagatc gattggatga ccgacctctc
      181 ttccacgccc gccatccccg tcaccatcca tcgcggcgat tatagcccgc ccgagtggca
      241 ggttccggac atcgcgctcg atttcgcgtt ggggctggag gaaacgaagg tcgcggcggc
      301 gctgtcggtc gtgcgccatg ccgatgcgcc cgcgccgctt gtgctgcgcg gcgatgggtt
      361 gacccccgcc gcggtgcgcg tcgacggcga ggtctggaac gactggcgca tcgacggcag
      421 tgacttgatc gtcgatctgg gcgagcggac ggcggcgatg gtcgaaatcg agacggtgat
      481 caatccggcg gcgaacaccc agctgatggg gctctatgcc tcgaacggta tgctctgcac
      541 ccagtgcgag gccgagggtt ttcgccgcat caccttccac cccgaccggc ccgacgtgct
      601 gagccgctac aaggtacgga tggaaggcga caaggcggcg ttcccgatcc tgttgtcaaa
      661 cggcaattgc atcgagcggg gcgagggcga agcggggacg cattgggcgc tttgggaaga
      721 tccatggccg aaaccctcct atctgttcgc gcttgtcgcg ggcgaccttg tcgtcaaccg
      781 cgacagcttc acgacgatgt cggggcgcaa ggtcgaactc ggcatctggg tgcgcgaggg
      841 cgatgtcgat cggaccagac atgcgatgga cgcgctcaag aacagcatga agtgggacga
      901 ggcggtctat ggccgcgaat atgacctcga cctgttcaac atcgtcgcgg tgagcgattt
      961 caacatgggg gcgatggaga ataaggggct caacatcttc aatacccgct acatcctcgc
     1021 cgaccccgac acggcgaccg acctcgacta tgacggggtc gagggcgtcg tcgcgcacga
     1081 atatttccac aattggtcgg gcaatcgcgt cacctgccgc gactggttcc aattgtcgct
     1141 gaaggaaggc ttcaccgtct ttcgcgacca gaatttctcg gccgacatgg gatcgccgcc
     1201 ggtcaaacgg atcgaggatg tccgcctgct ccgcgccgcg caattccccg aggatgccgg
     1261 cccgctcgcg catccgatcc gcccggatag ctatcaggaa atctcgaatt tctacaccgc
     1321 gaccgtctat aacaagggcg ccgaaatcat ccggatgatg catacgatgg tcggacccga
     1381 gcggttccgc aagggcaccg acctctattt cgagcgccac gacggcgagg cggcgacgtg
     1441 cgaggatttc gtccgtgcga tcgaggatgg cgccaaggtc gatctcgccc agttccgcct
     1501 gtggtatgga caggcgggca cgccgcgcat caccgcgcgg atcgacaccg acgcggcgac
     1561 gggcgatcac acgctgcatc tggcgcagct cgtcccgccg accccggggc agcccgacaa
     1621 ggcgccgatg ctgatcccgc tacgtatcaa ggcttatgat ccgggcagcg gcgatggcga
     1681 tgaggcggag caactcatac tgctcgaccg tgccgaaatg gcggtgccgc tgggtcctta
     1741 tcgcagccgg ccgatgcttt cgatcaaccg cggcttttcg gcgccggtga tcgtcgattt
     1801 cgcgcgtgag gacggcgagc ttgcctggct cgccgcgcat gacgacgatc cgttcgcacg
     1861 ttacgaagct ttgcagcaat tgatgctcga cacgctcgtc gccgcggtgt cggggaaggg
     1921 cggcggcagc ggcgcggtga tcgaagccgt ggcgcagacg ctggcgggcc gcgccgacga
     1981 ccccg
//
LOCUS       DATMSU010000343         1557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_24679, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000343 DATMSU010000000
VERSION     DATMSU010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24679"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VN034_09270"
     CDS             <1..523
                     /locus_tag="VN034_09270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWV60832.1"
                     /translation="TGLPNRRGFIDSVTPLLDRARADNLPAALLLVDIDRFKSINDIY
                     GHEAGDAVLCGIGRRIERWEGPMCTVARLGGEEFGLLTIGMEGIILGRFAESIRRGIA
                     ACDHSEAVGERLVTASVGVAEVRPACDFKQLYRLADEALYDAKRGGRNRVAVQRHQND
                     AGRASGREIAVSK"
     gene            complement(552..998)
                     /locus_tag="VN034_09275"
     CDS             complement(552..998)
                     /locus_tag="VN034_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWV60833.1"
                     /translation="MPDSESVPSLPPAVPAKMATGARHTTFSILGSDVVVTGNVAASV
                     DLHVDGKIEGDLKCANLVQGEASEIKGAVTAETAKIAGLLDGSIEAKTLIVHATARIT
                     GDVVYESITIENGGKVDGKLSHRRHGAAAAKAPPPLEVVENKSAAL"
     gene            complement(949..>1557)
                     /locus_tag="VN034_09280"
     CDS             complement(949..>1557)
                     /locus_tag="VN034_09280"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWV60834.1"
                     /translation="VRNAAAGRGGPFIPYRDRMGKAKTLGASFAALDSALFRMEVLER
                     TLVAVPSGNPANVLMMSSGFGYRSDPFTGAGAMHAGLDFRGPIGTPILAAAPGRVSFV
                     GRKSGYGNVVEVDHGQGILTRYAHLSGFTAKVGTQVTAGQQIAKMGSTGRSTGSHLHF
                     EVRLNGEAVNPRRFLEAKADVLEVKADARQRVGSVAAARGAR"
BASE COUNT          238 a          511 c          553 g          255 t
ORIGIN      
        1 gacgggcctg ccgaaccgcc gcggcttcat cgacagcgtg acgccgctgc tcgaccgcgc
       61 gcgcgccgac aatctgccgg cggcgctgct gctcgtcgat atcgaccgct tcaagtcgat
      121 caacgatatt tatggtcacg aggcgggcga cgcggtgctg tgcggtatcg gccgccggat
      181 cgagcggtgg gaaggcccga tgtgtaccgt cgcgcggctg ggcggcgaag agttcggcct
      241 gctgacaatc gggatggagg gcatcatcct cggccgcttc gccgaaagca tccgtcgcgg
      301 aattgccgcg tgcgatcata gcgaggcggt gggcgagcgg ctggtgaccg cgagcgtggg
      361 tgtggccgag gtgcggccgg cgtgcgattt caagcagctc taccggctcg ccgacgaagc
      421 cctttacgac gccaagcgcg gcggccggaa cagggtcgcc gtccagcggc accagaatga
      481 cgcggggcgc gcgtccggac gcgagatcgc cgtgtcgaaa tgatcgcggc cgcgccgacg
      541 cgcggcgccg gtcagagggc ggccgacttg ttctcgacga cttcgagcgg cggcggcgct
      601 tttgccgccg cggcgccatg gcggcgatgc gacagcttgc cgtcgacctt gccgccattc
      661 tcgatcgtga tgctttcata gaccacgtcg ccggtgatcc gcgcggtcgc gtggacgatc
      721 agcgtcttgg cctcgatcga cccgtcgagc agacccgcga tcttcgcggt ctcggcggtc
      781 accgcgccct tgatctcgct cgcctcgccc tggacgagat tggcgcattt gaggtcgccc
      841 tcgatcttgc cgtcgacgtg caggtcgacg ctcgccgcca cattgccggt gacgacgacg
      901 tccgatccga ggatcgaaaa ggtcgtgtga cgcgcgccgg tcgccatctt agcgggcacc
      961 gcgggcggca gcgacggaac cgactcgctg tcgggcatcg gctttgactt cgagaacatc
     1021 ggcttttgcc tccaggaatc tgcgtggatt gaccgcttcg ccgttcaggc ggacttcgaa
     1081 atgcaggtgg ctgccggtcg aacggccggt cgagcccatc ttggcgatct gctggccggc
     1141 ggtgacctgc gtgccgacct tggccgtgaa acccgacaag tgggcgtagc gcgtcaggat
     1201 gccctgacca tgatcgacct cgacgacatt gccatagccc gacttccggc cgacgaagct
     1261 gacgcggccg ggcgccgcgg cgaggatcgg ggtgccgatc ggcccgcgga agtcgagacc
     1321 cgcgtgcatc gcgcccgcgc cggtgaaggg gtcgctgcga tagccgaagc ccgacgacat
     1381 catcagcaca ttggcggggt tgcccgacgg caccgcgacg agcgtgcgtt cgagcacctc
     1441 catgcggaac agcgcgctgt cgagcgccgc gaagctggcg cccagcgtct tggctttgcc
     1501 catgcgatcg cgatagggga tgaagggacc accgcgaccg gccgcggcgt tgcggac
//
LOCUS       DATMSU010000344         5466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_138700,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000344 DATMSU010000000
VERSION     DATMSU010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138700"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1775)
                     /gene="serA"
                     /locus_tag="VN034_09285"
     CDS             complement(189..1775)
                     /gene="serA"
                     /locus_tag="VN034_09285"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWV60835.1"
                     /translation="MTAPKVLISEKMDPKAEAIFKERGIQVDVITGKTKDELIAMIGD
                     YDGLAIRSATKVTADVLAAATNLKVVGRAGIGVDNVDIPEASKKGVIVMNTPFGNSIT
                     TAEHAIAMMFALARQIPEANTGTQAGKWPKNDFMGVELTSKTLGLIGCGNIGSIVAER
                     ALGLRMKVVAFDPFLTPERAIELGVEKADLDTLLAKADFITLHTPLTDQTRNILSKEN
                     LAKAKKGVRIINCARGGLIDEAALKEALESGHVAGAALDVFQTEPPAADHPLFSAPNF
                     ICTPHLGASTDEAQVNVAIQVAEQISDYLLTGGITNALNVPSLSAEEAPKLRPYMSLA
                     EKLGSLVGQLAHDNLTHISIEVEGAAAELNMKPITAAVLTGLMRRYSDSVNMVNAPHL
                     ARERGLDVREVRHDREGDYHTLVRVTVATEAGDRSVAGTLFGNNEPRLVEMFGIKVEA
                     DLDGHMLYIVNEDAPGFIGRIGTALGEAGLNIGTFHLGRRAAGGEAVLLLSLDSPMPE
                     PLLWQLCQLPGVKTVKGLKF"
     gene            complement(1861..2988)
                     /locus_tag="VN034_09290"
     CDS             complement(1861..2988)
                     /locus_tag="VN034_09290"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HWV60836.1"
                     /translation="MSEVTTPQVRPTRPYFSSGPCAKPPVWSPEKLNTESLGRSHRAK
                     IGKTRLQYCIDLMREMLQLPDTHRIGIVPGSDTGAFEMAMWTMLGARPVTTLAWESFG
                     EGWVTDAAKQLKLDPTIIRADYGELPDLTKVDWSNDVLFTWNGTTSGVRVPNGDWIAA
                     DREGLSFADATSAVFAYDLPWDKIDVATFSWQKVLGGEGGHGVLILGPRAVERLETHT
                     PAWPLPKVFRLMSKGALAEGVFKGETINTPSMLAVEDAIFALEWAKSLGGLDGLKARS
                     DANAAALDKIVSEREWLSHLAADPASRSKTSVCLSVAGADADFIKKFAGLLEKEGAAY
                     DIAGYRDAPPGLRIWCGATVDTADVEALGPWLDWAYASLNA"
     gene            3160..3861
                     /locus_tag="VN034_09295"
     CDS             3160..3861
                     /locus_tag="VN034_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HWV60837.1"
                     /translation="MPIPTFLKPRILIAASAALALSACFGAPEAMKNSGGKRTSASKP
                     SRPQPVGTPSFTSAAAQQCAFDLKQAGVRFTPLPNEDHGGGCTSIDSVKLLDVGVPVS
                     ELGAMTCPLAKNFAAWAQYAVKPAARKFFGTEVVKIETYGTYSCRNIYGGRSGRLSQH
                     AYSNAIDVSGFVLADGRRIMLDGGWDGDKPSQDFLRALHKSGCRRFGTVLGPDYNAAH
                     YNHFHFDMSGNGYCR"
     gene            4096..4971
                     /locus_tag="VN034_09300"
     CDS             4096..4971
                     /locus_tag="VN034_09300"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HWV60838.1"
                     /translation="MAEDKQPHRSRFHKAKDDAQFAKQATTTPQTAHPAYKLAFQDKD
                     FLLREDLRPVRFQLELLKPELLLDEAGIESTLVIYGSARIPEPSQADGLEAAATDDAQ
                     RNIARRLKEKAKYYDEARALARLASQYPCDEKGCRHFVVCSGGGPSIMEAANRGADDE
                     GRESIGLNIVLPHEQAPNRFVTPSLSFQFHYFALRKMHFLLRARAVCVFPGGFGTFDE
                     MFELLTLIQTGKIKPIPIVLFGKEFWQRVVNFEALVEEGVVSARDLDLFRFVETADEA
                     WTIITDFYANIEENP"
     gene            complement(5155..>5466)
                     /locus_tag="VN034_09305"
     CDS             complement(5155..>5466)
                     /locus_tag="VN034_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HWV60839.1"
                     /translation="APTAAMGEQVFRRCVACHTVEKGGSNGIGPNLHGIVGAAVASKP
                     DFSYSGAMKAKGGVWDEAALDAYLEAPMKDVPGTRMAFAGVIDPADRKALILFLEEHS
                     K"
BASE COUNT          923 a         1726 c         1791 g         1026 t
ORIGIN      
        1 ggtcgcgcaa accttcgggc agcagggcag gggccttggt catgcgtgcg gccttagaac
       61 ggcgggggcc ggagtcaatg actcccctcc cacttgtggg aggggtcggg ggagggcccg
      121 tttggtcagg actgtgctga catgccctcc cctaacccct cccgcaagcg ggagggggat
      181 tgagtgtttt agaacttcaa ccccttcacc gtcttcacgc cgggaagctg gcacagctgc
      241 cacagcagcg gctcgggcat cggcgaatcg agcgacagca gcagcaccgc ttcgccgccc
      301 gcggcgcggc ggccgaggtg gaaggtgccg atgttgagcc cggcttcgcc aagcgcggtg
      361 ccgatgcggc cgatgaagcc cggggcgtcc tcgttgacga tgtagagcat atggccgtcg
      421 aggtcggctt cgaccttgat cccgaacatt tcgacgaggc gcggttcgtt gttgccgaac
      481 aacgtacccg cgaccgaccg gtcgcccgct tcggtcgcga cggtgacgcg gacgagcgtg
      541 tgatagtcgc cttcgcgatc atgacgaact tcgcgcacat cgaggccgcg ttcgcgcgcg
      601 agatgcgggg cgttgaccat gttcacgctg tccgaataac ggcgcatcag gccggtgagc
      661 accgcggcgg tgatcggctt catgttgagc tccgcagcgg cgccctcgac ctcgatcgag
      721 atgtgggtga gattgtcgtg cgcgagctgc ccgacaaggc tgccgagctt ttcggcgagg
      781 ctcatatagg ggcgcagctt cggcgcttcc tcggccgaca ggctggggac gttgagcgca
      841 ttggtgatcc cgccggtgag cagatagtcg ctgatctgct cggccacttg aatggcgaca
      901 ttgacctgcg cctcgtcggt cgacgcgccc agatgcggcg tgcagatgaa gttcggcgcg
      961 ctgaacagcg gatggtcggc ggcgggcggc tcggtctgaa acacgtcgag tgccgcgccc
     1021 gcgacatggc ccgattcgag cgcttccttc agcgccgctt cgtcgatcag cccgccgcgc
     1081 gcgcagttga tgatgcgcac gcccttcttg gccttcgcca gattttcctt cgacaggatg
     1141 ttccgcgtct ggtcggtcag cggcgtgtgc agcgtgatga agtcggcctt ggcgagcaag
     1201 gtgtcgaggt cggccttttc gaccccgagt tcgatcgcgc gttcgggggt caggaaggga
     1261 tcgaacgcga cgaccttcat gcgcaggccg agcgcgcgct cggcgacgat gctgccgata
     1321 ttgccgcagc cgatcaggcc gagcgtcttc gaggtcagct cgacgcccat gaagtcgttc
     1381 ttcggccatt tgcccgcctg cgttcccgtg ttcgcttcgg gaatctggcg cgcgagcgcg
     1441 aacatcatcg cgatcgcatg ttcggcggtg gtgatgctgt tgccgaaggg ggtgttcatc
     1501 acgatgacgc cctttttcga cgcctccgga atgtcgacat tgtcgacgcc gatcccggcg
     1561 cggccgacga ccttcaggtt cgtcgcggcg gcgaggacgt cggcggtgac tttggtcgcc
     1621 gagcggatcg cgaggccgtc atagtcgccg atcatcgcga tcagctcgtc cttggtcttg
     1681 ccggtgatca cgtcgacctg gatgccgcgt tccttgaaga tcgcttcggc tttggggtcc
     1741 attttttcgg aaatgagaac tttgggagcg gtcatgtctt gtctccgtca tcccggcgaa
     1801 agctgggatc gttgggaatg gggatgatcg atagcggtcc cagctttcgc tgggacgacg
     1861 tcaagcgttg agcgaggcgt aggcccagtc gagccacggg ccgagcgctt cgacgtccgc
     1921 ggtgtcgacg gtcgcgccgc accagatgcg cagccccgga ggcgcatcgc gatagcccgc
     1981 gatgtcatag gccgcgcctt ccttttcgag cagcccggcg aatttcttga tgaaatccgc
     2041 atcggcgccc gcgaccgaca ggcaaaccga ggtcttcgag cggctcgcgg gatcggcggc
     2101 gaggtggctc agccactcgc gttccgaaac gatcttgtcg agcgccgcgg cattggcatc
     2161 gctgcgtgcc ttcaggccat cgagaccgcc caaggacttc gcccattcga gcgcgaagat
     2221 cgcatcctcg accgcgagca tcgacggggt gttgatcgtc tcgcccttga acacgccttc
     2281 ggccagcgcg cccttcgaca tcaggcgaaa caccttgggc agcggccagg cgggtgtgtg
     2341 ggtttcgagt cgttcaaccg cgcgggggcc gaggatcagc acgccatggc cgccttcgcc
     2401 gccgagcact ttctgccagc tgaaggtcgc gacgtcgatc ttgtcccacg gcaggtcgta
     2461 agcgaacacc gccgaggtcg catcggcgaa gctgagccct tcgcggtcgg cggcgatcca
     2521 gtcgccgttc ggcacgcgca cgccgctggt ggtcccgttc caggtgaaaa gaacgtcgtt
     2581 cgaccagtcg accttggtca aatcgggaag ctcgccgtaa tcggcgcgga tgatcgtcgg
     2641 atcgagcttg agctgcttgg cggcgtcggt gacccagcct tcgccgaagc tctcccaggc
     2701 gagcgtcgtg acggggcggg cgccgagcat cgtccacatc gccatttcga atgcgccggt
     2761 gtcggaaccg ggcacgatgc cgatgcggtg cgtgtcggga agctggagca tctcgcgcat
     2821 caggtcgatg caatattgga gacgcgtctt gccgatcttc gcacgatgcg aacggccgag
     2881 cgattcggtg ttcagttttt cgggggacca gaccggcggc ttggcgcagg gtccggaaga
     2941 aaaatagggg cgcgtcgggc gcacctgtgg cgtcgtcact tcactcatat agtctctcct
     3001 tgcagagagc tcgcgcggcg ttgggaccgc gtggcccggc gccgcgttta gggttcgcgc
     3061 gcgggctgtc aaacaaattg cacggaccgc gcgaaatttt cgacgagtgg agaaaatagt
     3121 atacagcttg ttaaccattt cgtcggtatt ttgacgccca tgccgatccc gactttcctg
     3181 aagccccgga tcctgatcgc cgccagcgcg gcgctggcgc tttccgcctg tttcggcgcc
     3241 cccgaggcga tgaagaatag cggagggaaa cggacgtcgg cgagcaagcc gagccgcccg
     3301 cagccggtcg gcaccccgtc ctttaccagc gccgcggcgc agcaatgtgc gttcgacctc
     3361 aaacaggccg gcgtccgctt cacgccgctg ccgaacgagg atcatggcgg cggctgcacc
     3421 tcgatcgatt cggtcaagct cctcgatgtc ggcgtgccgg tgtcggagct cggcgcgatg
     3481 acctgtccgc tcgcgaagaa tttcgccgcc tgggcgcaat atgcggtgaa gcccgcggcg
     3541 cggaaatttt tcggcaccga ggtcgtgaag atcgaaacct atggcaccta tagctgccgc
     3601 aacatttatg gcgggcgttc ggggcggctg tcgcagcacg cctattcgaa cgccatcgac
     3661 gtgtcgggct tcgtgctcgc cgacgggcgc cgtatcatgc tcgacggcgg ctgggacggg
     3721 gacaagccgt cgcaggactt cctccgcgcg ctgcacaaat cgggctgccg ccgcttcggc
     3781 accgtgctcg gccccgatta caacgcggct cactataatc attttcactt cgacatgagc
     3841 ggcaacggct actgccgctg agctgtttcc ttctcccttg atgggagaag gatatggagc
     3901 cttgccgcga gaagcggtta ggcggagttg gatgagggtg atggtgcgtc ctcgcgccgt
     3961 catttcaggc cgagagttca ccctcaccgc tgcgactagc gaacgagttc gcaagtctcg
     4021 ctgcctcccc catcaagggg gaggatagcg tggcgcgtct tggcaaagtc acgccgaccg
     4081 gctagccgtc cgccaatggc agaagataaa caaccccatc gttcgcgttt ccacaaagcc
     4141 aaggacgacg cgcagttcgc caagcaggcg accaccaccc cgcagacggc gcaccccgcc
     4201 tacaagctcg catttcagga caaggacttc ctgctgcgcg aggatctgcg cccggttcgc
     4261 ttccagctcg aactgctcaa gcccgaattg ctgctcgacg aggcggggat cgaatcgacg
     4321 ctggtcatct acggctcggc gcgcattccc gaaccgtcgc aggccgatgg cctcgaagcc
     4381 gcggcgaccg acgatgcgca gcgcaacatc gcgcggcggc tgaaggaaaa agccaaatat
     4441 tatgacgagg cgcgcgcgct cgcgcggctc gccagccaat atccctgcga tgaaaagggc
     4501 tgccgccatt tcgtcgtctg ttcgggcggc gggccgtcga tcatggaggc cgcgaaccgc
     4561 ggcgccgacg acgagggccg cgaatcgatc ggtctcaaca tcgtgctgcc gcacgaacag
     4621 gcgccgaacc gcttcgtgac gccgtcgctg agctttcaat tccactattt cgcgctgcgc
     4681 aagatgcact tcctgcttcg cgcgcgcgcg gtctgcgtct tccccggcgg cttcggcacg
     4741 ttcgacgaga tgttcgaact gctgacgctg atccagacgg gcaagatcaa gccgatcccg
     4801 atcgtgctgt tcggcaagga gttttggcag cgcgtggtca atttcgaggc gctggtcgaa
     4861 gagggcgtgg tcagcgcgcg cgacctcgat ctcttccggt tcgtcgagac cgccgacgag
     4921 gcatggacga tcatcacgga tttctacgcg aacatcgagg aaaatccttg aggttccctc
     4981 ccccttagat gggggaggca gcgagacttg cgaacttgtt cgctagtcgc agcggtgggg
     5041 gtgcggtctc gccctgaagc gatggtgcaa ggacgcgcca tcacccccat ccaactccgc
     5101 ctaatcgctt cgcgataagg ctgcgtatcc ttcccccatc gagggggaag gcggctattt
     5161 cgaatgttct tccagaaaca ggatcagcgc cttgcgatcc gccgggtcga tcaccccggc
     5221 aaaggccatg cgcgtgcccg gaacatcctt catcggcgct tccagatagg cgtcgagcgc
     5281 ggcttcgtcc cagacgccgc ccttcgcctt catcgcaccc gaataggaaa agtccggctt
     5341 cgacgcgacc gccgcgccga cgatgccgtg cagattgggg ccgatgccgt tggacccgcc
     5401 cttttcgacg gtgtggcagg cgacgcagcg ccggaagacc tgttcgccca tcgccgccgt
     5461 cggcgc
//
LOCUS       DATMSU010000345         9936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_176521,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000345 DATMSU010000000
VERSION     DATMSU010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9936
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176521"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VN034_09310"
     CDS             complement(<1..120)
                     /locus_tag="VN034_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HWV60840.1"
                     /translation="MRTATVQRTTKETDIAIAVNLDGTGEYSVSTGIGFLDHMV"
     gene            208..690
                     /locus_tag="VN034_09315"
     CDS             208..690
                     /locus_tag="VN034_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HWV60841.1"
                     /translation="MSDDVRDSLIPYDEIVQDALRAVVGRVLSQIEGYDSLPGEHHFY
                     ITFKTQAPGVDIPAHLIAKFPDEMTIVLQNRFWDLSVEEDHFAVGLSFNQTPSMLTIP
                     YAAITAFVDPSVDFGLQFQVSDDEAAQPEPHDEADNDRPEVTSEDGSNVVTVDFGKRK
                     "
     gene            958..1272
                     /locus_tag="VN034_09320"
     CDS             958..1272
                     /locus_tag="VN034_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60842.1"
                     /translation="MTGPLWPPSRFWQYWALAGMLVLTGAFWWGVEGYALFEAGYAQS
                     QIADGLLRFSLLILTPALVIVWLVAAWLRRRVGETGYWQLLGLVAMIWAGAVLVTRML
                     VA"
     gene            1269..2666
                     /gene="fumC"
                     /locus_tag="VN034_09325"
     CDS             1269..2666
                     /gene="fumC"
                     /locus_tag="VN034_09325"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HWV60843.1"
                     /translation="MSATRTESDSIGDIEVAAEAYWGAQTERSRHNFAFPAHERMPLP
                     IVHALARIKGAAARVNRNHGLDAGIAEAIAEAAEAVAQGEHDEQFPLAIWQTGSGTQS
                     NMNANEVIAGIANEKLSGTRGGKTPVHPNDHVNMSQSSNDSFPTALHVAVAVETTARL
                     LPALAELTDALAAKSEAWGDIVKIGRTHLQDATPLTLGQEFSGYAQQLIACRTRIKGA
                     LAHDICKLAQGGTAVGTGLNAPAGFDVAIAAELSKSTGIAFEPASNKFEALASNDGLV
                     FFSGALNTLGVALSKIANDIRLLGSGPRAGLGELDLPANEPGSSIMPGKVNPTQCEML
                     TMVAAQVIGNTQAVTVGGLQGHLELNVFKPLIGSNILRSIELLSIGMAGFTEHCVVGI
                     KPNRGRIDELMNRSLMLVTALAPEIGYDKAAKIAKHAHETGSTLKEAALDLGFVDAAT
                     FDRVVDPRTMLGPEG"
     gene            2718..3086
                     /locus_tag="VN034_09330"
     CDS             2718..3086
                     /locus_tag="VN034_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60844.1"
                     /translation="MIGRIVGGVLGSAIGRRKGNHPVAGAVIGAGTLFAARRLFPQRY
                     AVLAATVAAGYLSKKWAERAEARKAAAEAHALDPELARAGVVDIYAGTAKVPTDGETM
                     GDALPPAIPVDGNGKRPALH"
     gene            complement(3278..3841)
                     /locus_tag="VN034_09335"
     CDS             complement(3278..3841)
                     /locus_tag="VN034_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 domain-containing protein"
                     /protein_id="HWV60845.1"
                     /translation="MMLGISRRGWLVAAALLAAFAAILFAMGRPPICPCGSVSLWHGT
                     VQSNQNSQQISDWYSFSHVIHGFIFFGLSRWLIPRQPLWVALALAIGIEAAWEILENS
                     PVIIDRYREVTMAYGYSGDSILNSVSDALFMILGFLAASRMRWWATVAIAIVFELFTL
                     WTIRDNLTLNVLMLVSPVEAVKEWQAE"
     gene            3890..4552
                     /gene="gmk"
                     /locus_tag="VN034_09340"
     CDS             3890..4552
                     /gene="gmk"
                     /locus_tag="VN034_09340"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWV60846.1"
                     /translation="MAESVHLNRRGVLFVLSSPSGAGKTTISRMMLEADKDIALSISA
                     TTRPPRPGEIDGVHYHFVDVETFKKMAADGEFLEWAHVFGHRYGTPRAPVDEMLEAGK
                     DVLFDIDWQGAQQLYQEAGPDVVRVFVLPPTMEELERRLRSRNTDSDEVIAARMERAA
                     NEISHWDGYDYVLINDNVEGCFDEVRAILRAERLKRRRQIGLIGFARDLIRSVPDADK
                     KL"
     gene            complement(4738..5175)
                     /locus_tag="VN034_09345"
     CDS             complement(4738..5175)
                     /locus_tag="VN034_09345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWV60847.1"
                     /translation="MAKPESWRLDAASYPVCIELQTRFQDMDINGHLNNVAFAALFES
                     GRVLLNRDVRPLDERPANERTMVAAVEINYLAEGNFPDPVQIATGIGRLGTSSWTIVQ
                     AMFQNGGCIATCDTVVVCRTDGQAKPLRAEMVAELEAKLARPV"
     gene            complement(5177..5959)
                     /locus_tag="VN034_09350"
     CDS             complement(5177..5959)
                     /locus_tag="VN034_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV60848.1"
                     /translation="MKLDSTVSAVVTGGASGLGAATARLLASKGVKVAIFDLQEEKGL
                     AIAAELGGVFCECNVTDDASVDAAFAKAREAHGQERILVNCAGTGNAIKTASRSKEDG
                     SIKHFPLDAFNWIIQINLVGTFRCIAKSAAGMLTLDPMEDGERGAIVNTASVAAEDGQ
                     IGQAAYSASKGGVVGMTLPIARDLGNENIRVNTILPGIFDTPLLAGAPQNVRDALGAS
                     VLNPKRLGNPDEYANLALCMIENGYFNGEDVRLDGGIRMAPR"
     gene            complement(6068..7237)
                     /locus_tag="VN034_09355"
     CDS             complement(6068..7237)
                     /locus_tag="VN034_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWV60849.1"
                     /translation="MATAYIVDAVRTAGGKRGGRLAGVHPVALGAAVFDAIADRNDFD
                     TKAIDDVITGCVSQGGQQTMDVGRNAVLASKLPDSIPAVTIDRQCGSSQQAIQFAAQA
                     VMSGTQDIVLASGIESMTRVPMGSVATLFMKEGLGHYKSERLEEKYPGIMFSQFMGAE
                     MIVKKHGLTKADLDAYALESHRRGAAATEAGHFKREIVGVEIDTPEGREIHLVDEGIR
                     FDATLEGIAGVKLLQEGGAITAASASQICDGASAALVVSEQALKDHGLTPRARIHHIS
                     VTAGDPVIMLEEPLFATERALKKAGMTIGDIDAYEVNEAFAPVPLAWLKYLGADPARI
                     NQHGGAIALGHPLGASGTKLMATLLGVLDATGGKYGLQTMCEGGGQANVTIVERL"
     gene            complement(7395..8549)
                     /locus_tag="VN034_09360"
     CDS             complement(7395..8549)
                     /locus_tag="VN034_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV60850.1"
                     /translation="MSLDSLSRSAYTEDHEAFRATVRQFLEKEVAPNAAQWAEDKIVP
                     KSIWPKAGELGMLCPTVPEEYGGLGLDFGYNAIVDEESAYYGRATTGFSLQSDIVTSY
                     IVKYGSEEQKKHWLPKMVSGEVITAIAMTEPGTGSDLQGMRTTAKKDGNHYVINGSKT
                     YITNGQNADLILVCVKTDTEVQPAWKGVSIVLVEADREGYQRGRNLDKIGQDEADTSE
                     MFFNDVRVPITNCLGEEGQGFIYLMSELPQERLSIAVSAQASAQRAFDDTVEFTRDRK
                     AFGKPILDFQNSRFVLADLKAKLQVGWAHLDWALARHMKKELTPEEGAAAKLWHTDLQ
                     WEVMDKCLQLHGGAGYMNEYPIARAWRAARVTRIFGGTNEIMKELIGRKL"
     gene            8852..9721
                     /locus_tag="VN034_09365"
     CDS             8852..9721
                     /locus_tag="VN034_09365"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWV60851.1"
                     /translation="MVQLLLDARALLGESPVWHAAEARLYWVDIDSRQIHRTDPVSGA
                     DEVMELAEQVGCIAPRAGGGLVAALENGCALIDSWDAAPRPFGPAVLADKPEQRFNDG
                     RVDTLGRLWVGSLTSDKANPAATLYRLDPDGSLTEMLHGLTTSNGAAFSPDGRIFYHA
                     DTPSHAIRAYDVDAAAGTLAGGRVFHQFEHGNGRPDGAAVDAEGCYWSALWDGWRVVR
                     FSPAGELLQTVELPVQRPTMIAFGGSDLKTAFITSAGKNLTDAERAKQPHAGGVFTFG
                     VDVPGLIQREFGA"
BASE COUNT         1682 a         3207 c         3275 g         1772 t
ORIGIN      
        1 gaccatatgg tcgaggaaac cgatgccggt ggacaccgaa tattcgcccg ttccgtcgag
       61 attgacggcg atcgcgatat ccgtttcctt ggtcgtgcgc tggacggtgg cggttcgcat
      121 ggcgcacgcc ttagagcctc attttcacgt tgcaaggcga ttctcccccc ttgaccggcg
      181 cgcggcgcgc gttcatcccg tcgcgtcatg agtgatgacg ttcgtgacag cctgattccc
      241 tatgacgaaa tcgtgcagga cgcgctgcgc gccgtggtcg gccgcgtgct gagccagatc
      301 gagggttatg acagcctgcc cggcgaacat catttctata tcaccttcaa gacgcaggcc
      361 cccggggtcg acatccccgc gcatctgatc gccaaattcc ccgacgaaat gacgatcgtg
      421 ctgcagaacc gcttctggga cctgagcgtc gaggaggatc atttcgccgt cggcctgtcg
      481 ttcaaccaga cgccgtcgat gctgacgatc ccctatgccg cgatcaccgc cttcgtcgat
      541 ccgtcggtcg atttcgggct gcagttccag gtcagcgacg acgaggccgc gcagcccgag
      601 ccgcacgacg aggccgacaa tgaccgtccc gaggtaacga gcgaggacgg gtcgaacgtc
      661 gtgacggtgg atttcggcaa gcggaaatag agggccagac tccgcccgca acgccttcga
      721 ggtttgaggc gattttgttc agggctagga ggcgaggcgc aggcatatga atatattcca
      781 aggctcgtcg acgcggccat agacaaaatc gaccaaatcc cgaagggacg ccgaaatggc
      841 ttcgatctcg cctccgcgtg gccttgcaaa tggaaccacc atccgcggcg gccacgcgca
      901 gccgatatct tcgccatttc gacgcttcga aggcgttgcg ggcggagtct ggcccggatg
      961 acgggcccac tctggccccc gagccgtttc tggcaatatt gggctctcgc ggggatgctg
     1021 gtcctgaccg gcgccttctg gtggggcgtc gagggttatg cgctgttcga ggcgggctac
     1081 gcgcaaagcc agatagccga tggcctgctc cgcttcagcc tcctgatact gactcccgcg
     1141 ctggtcatcg tctggctcgt cgccgcatgg ctgcgccgac gcgttggcga aacgggatat
     1201 tggcaattgc tcggcctcgt cgcgatgatc tgggccggcg cggttctggt gacgaggatg
     1261 ctggtggcat gagcgcaacg cggaccgaaa gcgactcgat cggtgatatc gaggtcgcgg
     1321 ccgaggccta ttggggcgcg cagaccgagc gcagccgcca taatttcgct tttcccgcgc
     1381 acgagcgcat gccgttgccg atcgtccatg cactcgcgcg aatcaagggc gccgcggcgc
     1441 gcgtcaaccg caaccatggg ctcgatgcgg ggattgccga ggcgatcgcc gaggcggccg
     1501 aggccgtcgc ccaaggcgag catgacgagc agttcccgct cgcgatctgg cagacgggca
     1561 gcggcaccca gtcgaacatg aacgccaatg aggtgatcgc ggggatcgcg aacgaaaagc
     1621 tcagcggaac gcgcggcggc aagacgcccg ttcaccccaa cgaccatgtc aacatgagcc
     1681 agtcgtcgaa cgacagcttt ccgaccgcgc tgcacgtcgc ggtcgcggtc gagacgacgg
     1741 cgcggctgct gcccgcgctg gccgaactga ccgacgcgct cgccgccaaa tccgaagcgt
     1801 ggggcgatat cgtcaagatc ggccgcaccc atttgcagga tgcgaccccg ctgacgctgg
     1861 ggcaggagtt ttcgggctat gcccagcagc tcatcgcctg ccgcacgcgg atcaagggcg
     1921 cgctggcgca tgatatctgc aagctcgcgc aaggcggcac cgcggtcggc accgggctca
     1981 acgcgcccgc gggcttcgac gtcgcgatcg cggcagagct ttcgaaaagc accggcatcg
     2041 ccttcgagcc ggcgagcaac aagttcgaag cgctggcatc gaacgacggg ctcgttttct
     2101 tctcgggcgc gctgaacacg cttggcgtcg cgctgagcaa gatcgcgaac gacattcgcc
     2161 tgctgggctc gggcccgcgc gcggggcttg gcgagctcga cctgcccgcg aacgagcccg
     2221 gcagctcgat catgccgggc aaggtcaatc caacccagtg cgagatgctg acgatggtcg
     2281 cggcacaggt gatcggcaac acccaagcgg tgaccgtggg cggcttgcag gggcatctcg
     2341 aactcaatgt cttcaagccg ctgatcgggt cgaacatact gcgttcgatc gagctgctga
     2401 gcatcggcat ggcgggcttt accgagcatt gcgtcgtcgg gatcaaaccc aatcgcggcc
     2461 gcatcgacga gctgatgaac cgctcgctga tgctcgtcac cgcgctggcg cccgaaatcg
     2521 gctatgacaa ggcggcgaag atcgcgaagc atgcgcatga gacgggcagc acattgaaag
     2581 aagccgcgct cgatctcggc tttgtcgatg ccgcgacctt cgaccgggtg gtcgatccgc
     2641 gcacgatgct ggggcccgag ggctgaacgg aacccgcggg gccttccggg gattgataaa
     2701 gggaaggaga ggcaaagatg atcggtcgca tcgtaggcgg cgttctcggc agcgccatcg
     2761 gacgccggaa gggcaatcat cccgtcgcgg gcgcggtgat cggcgcgggc acattgttcg
     2821 cggcgcggcg cctgttcccg cagcgctatg ccgtgttagc ggcgacggtt gccgcgggct
     2881 atctcagcaa gaaatgggcc gagcgcgccg aggcgcgcaa ggcggcggcg gaagcgcatg
     2941 cgctcgaccc cgaactggcg cgcgccggcg tggtcgacat ctatgcgggc accgcgaaag
     3001 tgccgaccga cggcgagacg atgggcgacg cgctgccccc cgcgattccg gtggatggca
     3061 acgggaagcg gccggcgctt cattgaaaac cccttccctc ttgatggggg aaggatacgc
     3121 agccttatcg cgaagcgatt aggcggagtt ggatgggggt gaaggcgcgt ccttgcgcca
     3181 cccgcgtcag gccgagaccg cacccccacc gctgcgacta gcgaacaagt tcgcaagtct
     3241 cgctgcctcc cccatcgagg gggagggaat cggacggtca ctccgcctgc cactccttca
     3301 ccgcctcgac cggcgagacc agcatcagca cattgagcgt cagattgtcg cggatcgtcc
     3361 acagcgtgaa gagctcgaag acgatcgcga tcgccaccgt cgcccaccag cgcatccggc
     3421 ttgccgcgag gaagccgagg atcatgaaga gcgcgtcgct caccgaattg aggatcgaat
     3481 cgcccgaata gccataggcc atcgtgactt cgcggtagcg gtcgatgatg acgggcgaat
     3541 tttcgaggat ttcccatgcc gcctcgatcc cgatcgcgag cgcgagcgcc acccagagcg
     3601 gctggcgcgg gatcagccag cgcgacaggc cgaagaagat gaagccgtgg atgacatggc
     3661 tgaagctgta ccagtccgaa atctgctggc tgttctggtt cgactggacg gtgccgtgcc
     3721 acaggcttac gctcccgcac gggcagatcg gcgggcgccc catcgcaaaa aggatggcgg
     3781 cgaaggccgc gagcagcgcc gcggcgacca gccagccacg tcgtgaaatc cccagcatca
     3841 tcgccccctt cccgcttgac cgcgcccgtc tctcgcgcaa ttagcgccca tggctgaaag
     3901 cgtccacctg aaccgccgcg gcgtgttgtt cgtcctctcc tcgccctcgg gcgcgggcaa
     3961 gaccaccatc tcgcgcatga tgctcgaggc cgacaaggat atcgcgctgt cgatctcggc
     4021 aaccacgcgc ccgccgcgcc ccggcgagat cgacggggtc cattatcatt tcgtcgatgt
     4081 cgaaaccttc aagaagatgg ccgccgacgg cgagttcctc gaatgggcgc atgtcttcgg
     4141 ccatcgttac ggcaccccgc gcgcgccggt cgacgagatg ctcgaagcgg gcaaggatgt
     4201 gctgttcgac atcgactggc agggcgcgca gcaactgtat caggaggcgg ggcccgatgt
     4261 cgtgcgcgtg ttcgtcctgc cgccgacaat ggaggaactc gaacggcggc tgcgcagccg
     4321 caacaccgac agcgacgagg tgatcgcggc gcggatggag cgcgcggcga acgagatcag
     4381 ccactgggac ggttatgact atgtgctgat caacgacaat gtcgaaggct gtttcgacga
     4441 ggtccgcgcg atcctgcgcg ccgagcggct gaagcggcgg cggcagatcg ggctgatcgg
     4501 ctttgcgcgc gacctgatcc ggtcggtgcc cgacgcggac aagaagcttt aatcatatcc
     4561 cctccccctt gatgggggag gcagcgaaac ttgcgaactt gttcgctagt tgcagcggtg
     4621 gaggtgcagt ttcggcctga aacgacggcg caagggcgca ccatcaccct catccaactc
     4681 cgcctagccg cttcgcgcca aggccccgta tccttctccc atcgagggag aaggaaatca
     4741 gaccggcctc gccagcttcg cttcgagctc cgccaccatc tccgcgcgca gcggcttcgc
     4801 ctgtccgtcg gtgcggcaca ccaccaccgt atcgcaggtc gcgatgcaac cgccgttctg
     4861 gaacatcgcc tggacgatcg tccagctcga cgtgccgagc cggccgatgc ccgtcgcgat
     4921 ctgcaccggg tcggggaaat tgccttcggc gagatagttg atctcgaccg cggcgaccat
     4981 cgtgcgctcg ttcgccgggc gctcgtcgag cggacgcacg tcgcggttga ggaggacgcg
     5041 gccgctctcg aacagcgcgg cgaaggccac attgttgagg tggccgttga tgtccatgtc
     5101 ctgaaagcgc gtctgcaatt cgatgcagac ggggtagctg gcggcatcga gccgccagct
     5161 ttccggtttc gccatatcag cgcggcgcca tgcggatgcc gccgtcgagg cggacgtcct
     5221 cgccgttgaa atagccgttc tcgatcatgc agagcgcgag attggcatat tcgtcggggt
     5281 tgccgaggcg cttcgggttg agcaccgagg cgccgagcgc gtcgcggacg ttctgcggcg
     5341 cgcccgcaag cagcggggtg tcgaagatgc cgggcaggat cgtgttgacg cggatattct
     5401 cgttacccag gtcgcgcgcg atggggagcg tcatgccgac gacgccgccc ttcgacgccg
     5461 agtaagccgc ctggccgatc tgcccgtcct cggccgcgac gctcgcggtg ttgacgatcg
     5521 cgccgcgctc gccatcttcc atcgggtcga gcgtcagcat gcccgccgcc gacttggcga
     5581 tgcagcggaa ggtgccgacg aggttgatct ggatgatcca gttgaaggca tcgagcggga
     5641 aatgcttgat gctgccgtct tccttcgagc ggctcgcggt cttgatcgcg ttgccggtgc
     5701 ccgcgcaatt gacgaggatg cgttcctgcc catgcgcttc gcgcgccttg gcgaaggcgg
     5761 cgtcgaccga cgcgtcgtcg gtgacgttgc actcgcagaa gacgccgccg agttcggccg
     5821 cgatcgcgag gcccttttct tcctgcaggt cgaagatcgc gaccttcacg cccttcgaag
     5881 cgagcaggcg cgcggtcgcg gcgccgaggc ccgaggcgcc gccggtgacg acggcggata
     5941 cggttgagtc gagtttcatg gattgtcctc tctgattttc gtcatcccag cgaaagctgg
     6001 gatcgcaggc gatggtgcgc ccaggtggat agcggcccca gctttcgctg gggcgacgct
     6061 ggattgctta caaccgctcg acgatcgtga cgttggcctg gccgccgcct tcgcacatcg
     6121 tctgcaggcc atatttgccg ccggtggcgt cgagcacgcc gagcagcgtc gccatcagct
     6181 tggtgcccga ggcgccgagc gggtggccga gcgcgatcgc gccgccgtgc tggttaatcc
     6241 gcgcgggatc ggcgccgaga tatttgagcc aggcgagcgg caccggcgcg aacgcttcgt
     6301 tgacttcata ggcgtcgatg tcgccgatcg tcatacccgc ctttttgagc gcgcgttcgg
     6361 tcgcgaacaa aggttcctcg agcatgatca ccgggtcgcc cgcggtcacc gagatgtgat
     6421 ggatgcgcgc gcgcggggtg agaccatgat ccttcagcgc ctgttcggag acgacgagcg
     6481 cggcgctcgc gccgtcgcag atctggctcg ccgacgccgc ggtgatcgcg ccgccctcct
     6541 ggagcagctt gacgccggcg atgccttcga gcgtcgcatc gaagcggatg ccttcgtcga
     6601 cgaggtgtat ttcgcggcct tcgggggtgt cgatctcgac cccgacgatc tcgcgcttga
     6661 aatggcccgc ctcggtcgcg gcggcgccgc ggcgatggct ttcgagcgca taggcgtcga
     6721 ggtcggcctt ggtgaggccg tgcttcttga cgatcatttc ggcgcccatg aactggctga
     6781 acatgatgcc ggggtatttt tcctcgagcc gctcggactt gtagtggccg aggccctctt
     6841 tcatgaacaa ggtcgcgacg ctgcccatgg gcacgcgcgt catgctttcg atgccgctcg
     6901 cgagcacgat gtcctgcgtt cccgacatca ccgcctgtgc ggcgaactgg atcgcctgct
     6961 gcgacgagcc gcactgacgg tcgatcgtca ccgcggggat cgagtcgggg agtttcgaag
     7021 cgagcacggc gttgcggccg acgtccatcg tctgttggcc gccttggctg acgcagccgg
     7081 tgatgacgtc gtcgatcgcc ttggtatcga aatcattgcg gtcggcgatc gcgtcgaaca
     7141 ccgcggcgcc gagggcgacg gggtggacgc ccgcgaggcg gccgccgcgc ttgccgcccg
     7201 cggtacggac agcgtcgacg atataggctg tggccatggg agaattcctt tcttcctaaa
     7261 gcccctcccc ttcaggggag gggttggggt ggggtctcgc gggttggggc aagccgatgg
     7321 ccccaccccg ctgcgactag cgggcaagcc cgcaagtctc gctgcccctc ccctgaaggg
     7381 gaggggtttg gtaattacag tttgcgcccg atcagttcct tcatgatctc gttcgtgccg
     7441 ccgaagatgc gcgtcacgcg cgcggcgcgc caggcgcggg cgatcggata ttcgttcata
     7501 tagcccgcgc cgccgtggag ctggaggcat ttgtccatca cttcccactg aaggtcggtg
     7561 tgccagagct tcgccgcagc gccctcctcg ggggtcagtt ccttcttcat gtggcgcgcc
     7621 agcgcccagt cgagatgcgc ccagccgact tgcagcttgg ccttcaaatc ggcgagcacg
     7681 aagcggctgt tctggaaatc gaggatcggc ttgccgaacg ccttgcggtc gcgggtgaat
     7741 tcgaccgtgt cgtcgaaggc acgctgcgcc gacgcctgcg cgctgaccgc gatcgacagg
     7801 cgttcctggg gaagctcgct catcagatag atgaacccct gcccctcttc gccgagacag
     7861 ttggtgatcg gcacgcggac gtcgttgaag aacatttccg acgtgtcggc ttcatcctga
     7921 ccgatcttgt cgaggttgcg cccgcgctga tagccttcgc ggtcggcctc gacgagcacg
     7981 atcgacacgc ccttccacgc cggctggacc tcggtgtcgg tcttgacgca gacgaggatc
     8041 aggtcggcgt tctggccatt ggtgatatag gttttcgacc cgttgatgac ataatgattg
     8101 ccatccttct tcgcggtcgt gcgcatgccc tgaaggtcgc tgccggtgcc cggttcggtc
     8161 atcgcgatcg cggtgatcac ctcgcccgac accatcttgg gcagccagtg cttcttctgc
     8221 tcctcgctgc catatttgac gatataggag gtgacgatgt ccgactggag cgagaagccc
     8281 gtcgtcgcgc ggccgtaata ggcgctttcc tcgtcgacga tcgcattata gccgaagtcg
     8341 aggccgagcc cgccatattc ctcgggaacc gtcgggcaca gcatgccgag ttcgcccgct
     8401 ttgggccaga tcgacttggg gacgatcttg tcctcggccc attgtgcggc gttcggcgcg
     8461 acttcctttt cgaggaactg gcgcaccgtc gcgcggaacg cctcatgatc ctcggtatag
     8521 gcggagcgcg agagactatc gagcgacata tggttccttt ccgtaaggca gccgaacgcg
     8581 agcggggagt cggccgcatc tttccgcgcc aagagacctt acgtttacgt ccacgtcaag
     8641 tagaaagacg ctacggcacg agactctttc gtcgccgccc tatctttcct cgtcatcccg
     8701 gacttgatcc gggatccatt ctttcaggcg cggtgtgaat ggaccccgga tcaagtccgg
     8761 ggtgacgaag gaggagccgc attctttcgt tactgccgaa taggtttgca gccgctgggc
     8821 agcatcggcg ggccgcgcta agacccgctc catggtccaa ctgctcctcg acgcccgcgc
     8881 cttgctcggc gaatcgcccg tctggcacgc cgccgaagcg cgcctctatt gggtcgatat
     8941 cgatagccgc caaatccatc gcaccgatcc cgtgagcggc gccgacgagg tcatggagct
     9001 ggccgagcag gtcggctgca tcgcgccgcg cgcgggcggc ggactggtcg cggcgctcga
     9061 aaacggctgc gcgctgatcg atagctggga cgcagcgccg cgccccttcg gccccgcggt
     9121 gctcgcggac aaacccgagc agcgcttcaa cgacgggcgc gtcgacaccc tcggccggct
     9181 gtgggtcggc agcctgacga gcgacaaggc gaatcccgcc gcgacgctct atcgcctcga
     9241 ccccgacggg tcgctgaccg agatgctgca tgggctgacg acgagcaacg gcgcggcgtt
     9301 cagtcccgac gggcgcatct tctatcacgc cgacacgccg agccacgcga tccgcgccta
     9361 tgacgtcgat gcggcggcgg gcacgctggc gggtggccgc gtcttccatc agttcgagca
     9421 tggcaacggc cgccccgacg gcgccgcggt cgatgccgag ggctgctatt ggtcggcgct
     9481 gtgggacggc tggcgcgtcg tgcgcttctc gcccgcaggc gaactgctgc aaacggtcga
     9541 actgccggtg cagcgcccga cgatgatcgc gttcggcggg tccgacctga agacggcctt
     9601 catcaccagc gcgggcaaga atctgaccga tgcggagcgc gcgaagcagc cgcacgcggg
     9661 cggcgtcttc accttcggcg tcgacgtgcc cggcctgatt cagcgggaat ttggcgccta
     9721 ggcaccttgc acccccccgt catctccggt taagacggcc cccgacatgg tttcgtccga
     9781 aaccttatcc tccggggaga tatgcttatg ccgcgcaccg cgcagccatt cgtccgtcat
     9841 ttgtcgatcc tgcttgcatc caccgcgatg atgacgggtt atagccagat gagcatcgca
     9901 accgccgccg aaccgaccgc cgccgccacg ccggcc
//
LOCUS       DATMSU010000346         5030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_186016,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000346 DATMSU010000000
VERSION     DATMSU010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186016"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VN034_09370"
     CDS             <1..399
                     /locus_tag="VN034_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWV60852.1"
                     /translation="VATRVALPMVAAGEIEGIFRRDGGNPVEGLRVELVDAEGRMRAA
                     TLTEFDGYFLFEGVAYGRYTVRLGKASAAALRLDGGFAMSAVPGKAVPRVRLGTVLLQ
                     PLRREVALQDGAPVDGNSARGPPGEGDAGR"
     gene            474..797
                     /locus_tag="VN034_09375"
     CDS             474..797
                     /locus_tag="VN034_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60853.1"
                     /translation="MTAVRERHDVRKAAWIVVSIGLLLAAGLFAALISAVPSPRNESG
                     AEPLIDRNPPQRTQNRQVYSPIIAKDPYVVDQWEKSIQALEAACAKRGEYCEEARQAR
                     RSVNR"
     gene            1026..2225
                     /gene="chrA"
                     /locus_tag="VN034_09380"
     CDS             1026..2225
                     /gene="chrA"
                     /locus_tag="VN034_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HWV60854.1"
                     /translation="MTTDLVPVPPSQLSLAALFLKFLRFGFLAFGGPVAQIAMIRHAL
                     VEEEGWIDPVRFNRLLAVLQVLPGPEAHELCVHLGMVARGRIGGVLAGLGFMLPGLAL
                     MLVAGWLYMGWIAGQAGLAGVLLGVQVVVLAIILRAVVRIGEHIVDDALLALVAAASM
                     LATIAGVPFWIPLVAGGLAYAASHRPAAAMLIVVGAAGLAASIHLTTSPAASGTGTMA
                     AAPVTLAALFVAGLKGGLLTFGGAYTAIPYVRSDTVGRGWIGDGTFLDGIALAGVLPA
                     PLVIFATFVGYVAGGLGGALAITAGMFLPAFAFSLILFERLEAIVENPALHRLLAGVA
                     AAVVGIIAATFVQLGQATGARVADSWFALLLFAAALAAVWRVKGAWVTPAIVAGGAIA
                     GWLGMSA"
     gene            complement(2485..3603)
                     /locus_tag="VN034_09385"
     CDS             complement(2485..3603)
                     /locus_tag="VN034_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWV60855.1"
                     /translation="MKPLEGIRVVELARVLAGPWCGQLLADLGAEVVKVERPGAGDDT
                     REWGPPFVTGDGGENLGAAYYHSCNRGKRSVAIDIASAEGQAEVRALLADADVVIENY
                     KVGGLVKYGLDPARLCADFPRLVVCSITGFGQTGPYARRAGYDYIVQAMSGFMSLTGE
                     PDGAPQKAGIAYADIFTGMYSAVAILAALRRRDVTGAGAHLDMALLDSQVAVLANQAA
                     NYLGSGVVPKRMGNAHVNVVPYQVFATADGQLVIAVGNDGQYRKLCAILGVPEWADDP
                     RFANNAARLANRGELIPLLAAKIAAWDAQPLSLALEAEGVPAGPINDLAQVFADPQVQ
                     ARGMRFRPEGASIDGVASPIVIDGERMVARVGAPPLGG"
     gene            complement(3605..4807)
                     /locus_tag="VN034_09390"
     CDS             complement(3605..4807)
                     /locus_tag="VN034_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV60856.1"
                     /translation="MALADPQRIEALDPLDPLQLDAELSDEERMVRDTARAYATDALL
                     PRVTSAFLDERFDREIMSEMGALGLLGATIDPEYGGAGLNYVSYGLIAREVERIDSGY
                     RSACSVQSSLVIHPINAYGSEEQKRKFLPGLTSGELVGCFGLTEPDAGSDPGGMRTRA
                     EKIEGGYRLKGAKMWITNSPIADVFVVWAKSEAHDGAIRGFLLEKGMKGLSAPKIEGK
                     VSLRASVTGEIVMDGVEVGEDALLPHVSGLKGPFGCLNRARYGIGWGAMGAAEFCYEA
                     ARNYTGERQQFGRPLAANQIVQLKLANMLTEIALGLQAAVRVGRLIEEGRLVPDAISL
                     LKRNNCGKALDIARVARDMHGGNGISADYHVIRHAVNLETVNTYEGTHDVHALILGRA
                     ITGISAFA"
BASE COUNT          762 a         1667 c         1714 g          887 t
ORIGIN      
        1 gtcgcgacgc gcgtggcgct gccgatggtg gcggcgggcg agatcgaggg gatattccgg
       61 cgcgacggcg gcaatccggt cgaggggctg cgcgtcgagc tggtcgatgc cgaggggcgg
      121 atgcgcgcgg cgacgctgac cgaattcgac ggttatttcc tgttcgaagg cgtcgcttac
      181 gggcgttata cggtgcggtt gggcaaggcg tcggcagcgg cgttgcggct cgatggcggt
      241 ttcgcgatga gcgcggttcc cggcaaggca gtgccgcgtg tgcggctggg gacggtgctg
      301 ctgcagccgt tgcggcgcga ggttgcgctg caggacggcg cgccggtgga cgggaacagc
      361 gcgcgcggcc cgccgggaga gggcgatgca ggacgctaac tggcccgaaa agccgacaat
      421 ttgcttgccg cgcccagccg ggaaagggat gaagcacggc gacgctgatc gcgatgacgg
      481 cggtccgtga aaggcatgat gtgcgaaaag cggcgtggat agtggtgtcg attggcctcc
      541 tgttagccgc aggcctcttt gcggcgctga tatccgccgt gccgtcgcct cgcaatgaaa
      601 gtggcgcgga gcctttgatc gaccgcaacc cgccccagcg cacgcaaaac aggcaggtct
      661 actcgccgat catcgcaaag gatccctatg tcgtcgatca atgggaaaaa tcgatccagg
      721 cgttggaggc ggcgtgcgcc aaacgcggcg aatattgcga agaggctcgg caagcgcgcc
      781 gatcggtaaa cagatagcgg cgctgcggct cgacggcggc tttgcgacga gcgcgggtcc
      841 gggcaaggcg acgccgcgca tatggccgtg atcgccaagg aggtgggcgg actcgggcgc
      901 tgcgattgac atcgccgccg cttcggatga tatcggcggc aacgggccga cgcggacgcc
      961 cgttcaggcg agggctcgcc caagttccgc tccccgccgc gcttcgcggc gctcggagcg
     1021 ttgagatgac caccgacctt gtccccgtcc cgccgtcaca gctcagcctg gcggcgctgt
     1081 tcctgaaatt cctgcgtttc ggtttcctcg cctttggcgg tccggtcgcg cagatcgcca
     1141 tgatccggca cgcgctcgtc gaggaggaag gctggatcga ccccgtgcgt ttcaaccgcc
     1201 tgctcgcggt gctgcaggtt ctgcccgggc ccgaagcgca cgagctttgc gtgcatttgg
     1261 gcatggtcgc gcgcgggcga atcggcggcg tgctcgcggg gctggggttc atgctgcccg
     1321 ggctggcgct gatgctggtt gcgggctggc tctatatggg ctggatcgcc ggacaggccg
     1381 ggctcgcggg cgtgctcctc ggtgtgcagg tcgtggtgct cgcgatcatc ctgcgcgccg
     1441 tggtgcggat cggcgagcat attgtcgacg acgcgctgct tgcgcttgtc gccgcggcaa
     1501 gcatgctcgc gacgatcgcg ggggtgccct tctggattcc gctcgttgcc ggggggctgg
     1561 cttatgccgc ttcgcatcgc cccgccgcgg cgatgctgat cgtggttggg gccgccggtt
     1621 tggcggcatc gatccacctg acgacgagtc ccgccgcgtc cgggaccggg acgatggcgg
     1681 ctgctcccgt cacgctcgcc gccttgttcg tcgcggggct gaagggcggg ctgctcacct
     1741 ttggcggtgc ttataccgcg attccctatg ttcgctccga cacggtcggg cgcggctgga
     1801 tcggcgacgg gacgttcctc gacggcatcg cgctcgcggg cgtgctgccg gcgccgctcg
     1861 tcattttcgc gaccttcgtc ggctatgtcg ccggggggct gggcggtgcg ctcgctatca
     1921 ccgcggggat gttcctgccc gccttcgcct tttcgctgat cctgttcgag cggctcgagg
     1981 cgatcgtcga gaatccggcg ctgcaccgcc ttctcgcggg ggtagctgcg gcggtggtgg
     2041 gcatcattgc cgcgaccttc gtgcaattgg gacaggcgac cggagcgcgg gtcgcggatt
     2101 cctggttcgc gctgttgctg ttcgcggcgg cgctggcggc ggtgtggcgc gtcaagggcg
     2161 cgtgggtcac gccggcgatc gtcgcaggcg gggcgattgc gggttggctg ggaatgtcgg
     2221 cttagggggg agctgccatt cccaacattc gtcattcccg cgaaagcggg aacccagtct
     2281 gggatcagcc tacgcacgct ctgggtcccc gctttcgcgg ggatgacgat gtaagaggtc
     2341 acaatcggtc gaatgccgtc ttccgaatat ttcgcgcaga gacgcagaga gcgcaaagag
     2401 ggaagctttt cacctctgcg atctctgcgt ctctgcgcga atcttattca aagccgccta
     2461 cgctgccatg gcagctaccg gccgttagcc gccaagcggc ggcgccccga cccgcgccac
     2521 catccgctcg ccatcgatca cgatcgggct cgcgacgccg tcgatcgacg caccttcggg
     2581 ccgaaatcgc atcccgcgcg cctgcacctg cggatcggca aacacttgcg ccagatcgtt
     2641 gatcggcccc gccggcacac cctcggcctc gagcgccaat gacagcggct gcgcgtccca
     2701 cgcggcgatc ttcgccgcga gcagcgggat cagctcgccc cgattcgcga gccgcgcggc
     2761 attattcgcg aaacgcggat cgtcggccca ttcgggcacg cccaatatcg cgcaaagctt
     2821 gcgatactgc ccgtcattgc cgaccgcgat caccagctgg ccgtcggcgg tcgcgaagac
     2881 ctgatagggc acgacattga cgtgcgcatt gcccatccgc ttcggcacga caccgctccc
     2941 cagataattg gccgcctgat tggcaagcac cgcgacctgt gaatcgagca gcgccatatc
     3001 gagatgcgcg cccgcgcccg tgacgtcacg gcgtcgcagc gcggcgagga tcgccaccgc
     3061 cgaatacatg ccggtgaaga tgtcggcata ggcgattcca gccttttgcg gcgcgccgtc
     3121 gggctcgccg gtcagcgaca tgaagccgct catcgcctgg acgatataat cataacccgc
     3181 gcggcgcgca taagggccgg tctggccgaa gcccgtgatc gaacagacga ccagccgcgg
     3241 gaaatcggcg cagagccgcg ccgggtcgag cccatatttg accagcccgc cgaccttgta
     3301 attctcgatc acgacatcgg catcggcgag cagcgcgcgc acctctgcct gcccctcggc
     3361 gctcgcgata tcgatcgcga cgctgcgctt gccgcggttg cagctgtgat aataggccgc
     3421 gcccagattt tcgccgccgt cgccggtgac gaaaggcgga ccccactcgc gcgtatcgtc
     3481 gcccgcgccc ggccgctcga ccttcacgac ctcggcgccg agatccgcga gcaattgccc
     3541 gcaccacggc cccgcgagca cccgcgccag ttcgacgaca cggatgccct ccagcggctt
     3601 cacattatgc aaaggcgctg atgcccgtga tcgcgcggcc gaggatcagc gcatggacgt
     3661 cgtgcgtgcc ctcataggtg ttcaccgttt cgaggttcac cgcgtggcgg atgacatgat
     3721 agtcggccga aatgccgttg ccgccgtgca tatcgcgcgc gacgcgggcg atatcgagcg
     3781 ccttgccgca attgttgcgc ttcaagaggc tgatcgcatc ggggacgagc cgcccctctt
     3841 cgatcagccg gccgacgcgc accgccgcct ggagcccgag ggcgatttcg gtgagcatgt
     3901 tggcgagctt caattgcacg atctggttcg ccgcgagcgg gcgtccgaac tgctggcgct
     3961 caccggtata attgcgcgct gcctcgtagc agaattcggc agcacccatc gcgccccagc
     4021 cgatgccgta gcgcgcgcgg ttgagacagc cgaacggccc cttgagcccc gagacatgcg
     4081 gcagcagcgc atcctcgccg acctcgaccc cgtccatcac gatctcgccg gtgaccgagg
     4141 cgcgcaagga caccttgcct tcgatcttcg gtgccgacag gcccttcatg cccttttcga
     4201 gcaggaaacc gcgaatcgcg ccgtcgtgcg cctccgactt cgcccacacg acgaaaacgt
     4261 cggcgatcgg cgaattggtg atccacatct tcgcgccctt gaggcgatat ccgccttcga
     4321 tcttttcggc gcgggtgcgc atcccgccgg gatcgctgcc cgcgtcgggc tcggtcaggc
     4381 cgaagcagcc gacgagttcg cccgaggtca gcccgggcag gaacttccgc ttctgctcct
     4441 cactgccata agcattgatc gggtggatca cgaggctgct ctgcaccgaa caggccgacc
     4501 ggtaccccga atcgatgcgt tcgacctcgc gcgcgatcag cccgtagctg acatagttca
     4561 gccccgcgcc gccatattcg gggtcgatcg tcgcgccgag caggccgagc gcgcccattt
     4621 cggacatgat ctcgcggtcg aagcgttcgt cgagaaaggc cgaagtcacc cgcggcaaca
     4681 gcgcatcggt cgcataggcg cgcgcggtgt cgcggaccat ccgctcctcg tcgctcagtt
     4741 cggcgtcgag ctggaggggg tcaagcgggt cgagcgcttc gatgcgctgc ggatcggcga
     4801 gggccatggg tctgctttct tcaaaaagcg acccatgggc ccgggttacg ggtatggcgt
     4861 cgctacgggg tcggttcggc cgttttgcgg gccaccgcgg caggcgggct gtcgcgcggc
     4921 tccaatatcg ccgccgtctc gatcaggtca agcgcgcgcc gcgcctcgtg atagcgccgc
     4981 gcatccatca gccattcgcc cgcgacgtcg gcgccgggca tcgcttcgac
//
LOCUS       DATMSU010000347         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_53301, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000347 DATMSU010000000
VERSION     DATMSU010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53301"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VN034_09395"
     CDS             complement(<1..647)
                     /locus_tag="VN034_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWV60857.1"
                     /translation="MAVTKAASLADDLVQRFEEQIETGEMPVGSRFPTEKEITETFGV
                     SRTVVREAYSRLAARGLLVSRRGSGAYVPDGAQYRAFQVTAEEIGEIDDVLRLLEMRM
                     GFEVEMADLAARRRTDADLAALRRTLDAMEESRDVDASVAADAAFHAAIASATGNPYY
                     LRFTQFLGVRLVPSRWLYLQGDDPVQHQRYAHTINRDHEAIVVAIEAGDPVAARRA"
     gene            complement(762..1973)
                     /gene="manD"
                     /locus_tag="VN034_09400"
     CDS             complement(762..1973)
                     /gene="manD"
                     /locus_tag="VN034_09400"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HWV60858.1"
                     /translation="MPKIVSARVILTSPGRNFTTLKIECDDGTTGVGDATLNGRELAV
                     AAYLSEHVVPCLIGRDAHRIEDIWQFLYKGAYWRRGPVTMAAIAAVDMALWDIKGKLA
                     GLPVYQLLGGAAREGCMVYGHANGATIADTIEAALDYQRQGYKAIRLQCGVPGMASTY
                     GVSKDRYFYEPADNDLPTENIWSTAKYMRVVPDLFAAAREALGWDVHLLHDIHHRLTP
                     IEAARLGKDLEPYRPFWIEDATPAEDQEAFRLIRQHTTTPLAVGEIFSSIWDCKHLIE
                     NRLIDYIRATVLHAGGITHMRQIAALADLHQVRTGCHGATDLSPVTMAAALHLGLSIP
                     NFGIQEYMRHTAETDAVFPHAWRFADGMLHPGDAPGLGVEIDEALAETFPYSRAYLPV
                     NRLEDGTMWSW"
BASE COUNT          393 a          721 c          708 g          376 t
ORIGIN      
        1 gcgcggcgcg cggcgacggg gtcgcccgcc tcgatcgcga cgacgatcgc ctcgtgatcg
       61 cgattgatcg tgtgcgcgta acgttgatgc tgaaccggat cgtcgccctg cagatagagc
      121 caccgcgacg gcacgagccg cacgccgagg aactgggtga agcgtaggta atagggattg
      181 ccggtcgcgc tggcgatcgc ggcatgaaag gcggcgtcgg cggcgaccga cgcgtcgacg
      241 tcgcggctct cctccatcgc gtcgagcgtc cggcgcagcg ccgcgaggtc ggcgtcggtc
      301 cgccgccgcg ccgcgaggtc ggccatctcg acctcgaacc ccatgcgcat ttcgagcagc
      361 cgcagcacat cgtcgatctc gccaatctcc tcggcggtga cctgaaaggc gcgatattgg
      421 gcgccgtcgg ggacataggc gcccgatccg cgccgcgaga ccagcaggcc gcgcgccgcg
      481 agccgcgaat aagcctcgcg cacgacggtg cggctgacgc cgaacgtctc ggtgatctct
      541 ttttcggtcg ggaaacgcga tccgacgggc atttcgcccg tttcgatctg ttcctcgaaa
      601 cgctggacca gatcatcggc cagcgatgcc gccttggtta ccgccatgga ccgagcgtta
      661 tcatcgcgcc gccaagctgg ctagggcccg tgcccatgaa ttacgcgttc gaagtttgag
      721 gcgatttgtt cggtgcgaga cggcaaggat cgcccaccac atcaccagct ccacatcgtg
      781 ccgtcctcga gccggttcac cggcagatag gcgcgggaat agggaaaggt ttcggcgagc
      841 gcctcgtcga tctcgacgcc gaggccgggt gcatcgccgg gatgcagcat gccgtccgcg
      901 aagcgccagg cgtgcggaaa gaccgcgtcg gtctcggccg tgtggcgcat atattcctgg
      961 ataccgaaat tgggaatcga aagccccaaa tgcagcgccg ccgccatcgt caccggcgac
     1021 aggtcggtcg cgccgtggca accggtgcgg acttggtgga ggtcggcgag cgcggcgatc
     1081 tggcgcatat gggtgatccc gcccgcgtgg agcacggtcg cgcggatata gtcgatcagc
     1141 cggttctcga tcagatgttt gcagtcccag atcgacgaga aaatctcgcc gaccgcgagc
     1201 ggcgtcgtcg tatgctggcg gatcaggcgg aacgcctcct gatcctccgc cggggtcgca
     1261 tcctcgatcc agaaggggcg ataaggctcg aggtccttgc cgagccgcgc cgcctcaatc
     1321 ggggtcagcc ggtgatggat gtcgtggagc agatgcacgt cccagccgag cgcctcgcgc
     1381 gccgcggcga aaaggtcggg gacgacgcgc atatatttcg cggtcgacca gatattctcg
     1441 gtcggcagat cattgtcggc cggttcgtag aaatagcggt ccttgctgac gccataggtc
     1501 gacgccatcc ccggtacccc gcattgcaga cgaatcgcct tgtaaccctg ccgctggtaa
     1561 tcgagcgccg cctcgatcgt atcagcgatc gtcgctccgt tggcatggcc atagaccatg
     1621 cagccctcgc gcgccgcgcc gccgagcaat tgatagaccg gcagccccgc gagcttgccc
     1681 ttgatgtccc acaaagccat gtcgaccgcc gcgatcgccg ccatcgtcac cggcccgcgg
     1741 cgccaatagg cgcccttgta gagaaattgc cagatatcct cgatccggtg cgcgtcgcgc
     1801 ccgatcagac aggggacgac atgctccgaa agataggccg ccaccgcaag ctcgcggccg
     1861 ttgagcgtcg catcgccgac gccggtcgtg ccgtcgtcgc attcgatctt cagcgtcgtg
     1921 aaattccggc ccggcgaggt caggataacc cgggctgaca cgatcttcgg cattccatcc
     1981 cctttggcca catgcgctag ttgtgtgaca acattattga cacacgaccg aaaagaaaag
     2041 agatatgtca ccggtgtcaa ggcgaaaagc ccgccgcgtt gaaagggaga ggatatgcca
     2101 tgtcgctgag cctgttttcc atgatgatga ccgctcccgc cgccttggcg gcgcctgccg
     2161 agcccgcgat gccgcagccc gccgcgatcc tcgccgat
//
LOCUS       DATMSU010000348         3423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_100708,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000348 DATMSU010000000
VERSION     DATMSU010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100708"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VN034_09405"
     CDS             complement(<1..546)
                     /locus_tag="VN034_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HWV60859.1"
                     /translation="MATLMSGSAVRLPRALPSWLRVRRRTPREIGPHLLVGLLGALLI
                     WQGVRLLWTLVTPLSPLGAWQPQTAVIASPAERRALFTSLDPFFRGQVQGPATATVTS
                     AGLTLFGINLNEATGGGSAIIAGEDGVQTSYAVGDEIASGLKLAGVAFDHVLLDRGGA
                     RESLFLDQSGEAPVASPGTALP"
     gene            complement(814..>3423)
                     /locus_tag="VN034_09410"
     CDS             complement(814..>3423)
                     /locus_tag="VN034_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60860.1"
                     /translation="DPAPAETPPADQADDGAAGEEEVDVSIPGGADAEIVVTGRYTPN
                     VVRATPEVVSVLSSADIARTGEGDISGSLQRVTGLSVVGGGFVYVRGLGDRYSLALLN
                     GSPLPSPEPLKRVVPLDIFPTNVIDSTLVQKSYSVNFPGEFGGGVINLTTKATPRETF
                     LTFSGGIGWDSETTNELGYTYYGSDTDWTGFDDGTRDVPPLLKAAFASGKPILEGADF
                     SLADLKAIQMELVNSETSLLQQNDHIPVNWSAGITGGTTIQLPDGELGIIATAGISNK
                     WRTRDTLQQTSLETDLSGEPQTNFNRVVTDNRIVVNGLLGFGLELGDHKFRWTNLYIR
                     DTLKQARLGLGTNRNQSDRDIMQQDTAWYERQLINTQLVAEFDFDRIQLDLRGGYANS
                     QREAPYERSFTYVRTNLPTDIDPVGDKFVNDLTGGRGSASISFSDLNEDLWSGGVDLS
                     YELAPRITATVGYAYSDTHRTSVRRDFLFRAQNLPIEVQQLRPDYLLSDASIQLYDIR
                     LLETSAQDGAAAFDANLTTHAGYAQLQAEVVPGVNINAGVRYEDAKQTVVPVDLFNTG
                     SSVFVPTNLNNDYWLPAVTLTWEMAPDMQLRLNGSKTIARPQFRELVAQVYLDPESNR
                     LYRGNPSLSDSELWNAELRYEWYFDRDQRFTVAGFYKSIDKPIEAYTSASDNQVSTSY
                     ANAPKATLYGAEVEVQKYFPLDTLSDAPFWQSRRLVLIGNYTYTQSDIQVGEDDVTFI
                     NGVERPADIFFFDGSPLTGQSDHLVNFQIGLEDQDRLSQQTLLLTYASPRVTSRGPSG
                     QPDLKEKPGIQLDFVARQGINLLNKEIELKFEARNLTGRKYEELQTSGDNKIYFNRYK
                     LGRTFSLSASIKF"
BASE COUNT          579 a         1016 c         1209 g          619 t
ORIGIN      
        1 gggaagcgcg gttcccggac tggcgacggg cgcctcgccg ctctggtcga gaaacaggct
       61 ttcgcgcgcg ccgccgcggt cgagcagcac atggtcgaac gcgacgccgg cgagtttcag
      121 ccccgacgcg atctcgtcgc cgacggcata gctcgtctgc accccgtcct cgcccgcgat
      181 gatcgccgaa ccgccgcccg tcgcctcgtt gaggttgatc ccgaacagcg tgagccccgc
      241 ggaggtcacg gtggcggtgg cgggaccctg aacttggccg cggaaaaagg gatcgaggct
      301 ggtgaagagc gcgcgccgtt cggcgggcga tgcgatgacg gcggtttgcg gctgccacgc
      361 accgagcggc gacagcggcg tgaccaacgt ccagagcagg cggacgcctt gccagatcag
      421 gagcgcgccg agcagaccga cgagcaggtg cggcccaatt tcgcgcgggg tccgcctgcg
      481 gacgcggagc cacgaaggca gcgcgcgcgg cagccgaacg gccgatccgg acatcagcgt
      541 tgccatggtt tcttccctgt cccccaactg aaaggctgca tgacgaatcg cctaggggcg
      601 attggtgaca atcagttgac ggcaagatta cagttttttg tcagccgggc gagtccggtg
      661 cggtggggcg cgagtaagcc cctccccttc aggggagggc agcgagactt gccggcttgc
      721 cggcttagtc gcagcgggtg gggccatcga ccttgcgcaa ggccgatggc cccaccccgc
      781 cccctcccct gaaggggagg ggcttttttt agcctaaaac ttgatcgaag cgctgagcga
      841 gaaggtgcgg cccagcttgt agcggttgaa atagatcttg ttgtcgcccg aggtctggag
      901 ctcttcatat ttgcgccccg tgaggttgcg cgcctcgaac ttcagctcga tttccttgtt
      961 caggaggttg atgccctgcc gcgcaacgaa gtcgagctgg atgccgggct tttccttgag
     1021 gtcgggctgg cccgacggac cgcggctggt gacgcgcggg ctggcatagg tcaggagcaa
     1081 cgtctgctgc gacagccggt cctgatcctc gagcccgatc tggaaattga ccagatggtc
     1141 cgactggccg gtcagcggcg agccgtcgaa gaagaagatg tcggccggac gctcgacgcc
     1201 gttgatgaag gtgacgtcgt cctcgccgac ctgaatgtcc gactgggtat aggtgtagtt
     1261 gccgatcagg acgagccggc ggctctgcca gaaaggcgcg tcggacagcg tatcgagcgg
     1321 gaaatatttc tgtacctcga cctcggcacc ataaagcgtc gccttcggcg cattggcgta
     1381 gctggtgctg acctgattgt ccgacgccga cgtataggcc tcgatcggct tgtcgatcga
     1441 tttgtagaag cccgcgacgg tgaaccgctg atcgcggtcg aaataccatt cgtagcgcag
     1501 ttcggcgttc cacagctcgc tgtcggacag cgaggggttg ccgcgataga ggcggttcga
     1561 ttcgggatcg agatagacct gcgcgaccag ctcgcggaac tgcggccgcg cgatcgtctt
     1621 cgacccgttg aggcgaagct gcatgtcggg cgccatttcc caggtcagcg tcaccgccgg
     1681 cagccaatag tcgttgttga gattggtcgg cacgaacacc gacgagccgg tgttgaacag
     1741 atcgaccggg acgacggtct gcttggcgtc ttcatagcgg acgccggcgt tgatgttgac
     1801 gcccggcacg acttcggcct ggagctgcgc atagcctgcg tgggtcgtca gattggcgtc
     1861 gaacgccgcc gcgccgtcct gcgccgaggt ttcgagcagc cggatgtcgt aaagctggat
     1921 cgaggcgtcg gagagcagat agtcggggcg cagttgctgg acctcgatcg gcagattctg
     1981 cgcgcggaac aggaagtcgc ggcgcaccga ggtgcgatgc gtgtcgctgt aggcgtaacc
     2041 gaccgtcgcg gtgatgcgcg gcgccagttc ataggagagg tcgacgccgc ccgaccagag
     2101 atcctcgttc agatccgaga aggaaatgct cgccgaaccg cggccgccgg tcaagtcgtt
     2161 gacgaacttg tcgccaacgg gatcgatgtc ggtgggcagg ttggtgcgca cataggtgaa
     2221 gctgcgctca tagggcgcct cgcgctgcga attggcatag ccgccgcgca ggtcgagctg
     2281 gatccggtcg aaatcgaatt cggcgacgag ctgtgtgttg atcagctggc gttcgtacca
     2341 cgccgtgtcc tgctgcatga tgtcgcggtc ggactggttg cggttggtgc cgaggccgag
     2401 gcgcgcctgt ttcagcgtgt cgcggatata gaggttggtc cagcggaact tgtgatcgcc
     2461 gagttcgagc ccgaaaccga gcaggccgtt gacgacgatg cggttgtcgg tgacgacgcg
     2521 gttgaaattc gtctgcggct cgcccgacag gtcggtttcg agcgaggtct gctgaagcgt
     2581 gtcgcgcgtg cgccacttgt tgctgatgcc cgcggtggcg atgatgccga gctcgccgtc
     2641 ggggagctgg atcgtggtgc cgccggtgat ccccgccgac cagttgaccg ggatatggtc
     2701 gttctgctgg agcaggctcg tctcggaatt gacgagctcc atctggatcg ccttcagatc
     2761 ggcgaggctg aaatcggcac cctcgaggat cggcttgccg ctggcgaagg cggccttgag
     2821 cagcggcggg acgtcgcgcg tgccgtcgtc gaagccggtc cagtcggtgt cgctgccata
     2881 ataggtatag ccgagctcgt tcgtcgtctc gctgtcccag ccgatgccgc ccgagaaggt
     2941 cagaaaggtc tcgcgcgggg tcgccttggt cgtgaggttg atcacgccgc cgccgaactc
     3001 gccagggaaa ttgaccgaat agcttttctg caccagcgtc gaatcgatga cgttggtcgg
     3061 gaagatgtcg agcgggacga cgcgcttcag cggttcgggg ctgggcagcg gcgagccgtt
     3121 gaggagcgcg agcgagtaac gatcgccgag gccgcggaca tagacgaaac cgccgccgac
     3181 gaccgacagg ccggtcacgc gctgcagcga gcccgagata tcgccttcgc cggtacgcgc
     3241 gatgtcggca gacgagagga ccgacacgac ctcgggcgtc gcgcggacga cattgggggt
     3301 atagcggccg gtcacgacga tttcggcatc ggcgccgccg ggaatcgaca catcgacctc
     3361 ttcttcgccg gccgcgccat cgtcagcctg gtcggcgggc ggcgtctcgg cgggcgcggg
     3421 atc
//
LOCUS       DATMSU010000349         6817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_34469, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000349 DATMSU010000000
VERSION     DATMSU010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6817
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34469"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..173
                     /locus_tag="VN034_09415"
     CDS             <1..173
                     /locus_tag="VN034_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HWV60861.1"
                     /translation="LRDRAGRPMPRWRGCRRLRDDAVLLLMEAPASFDGRYFGPTARC
                     DIIGKATPLWLR"
     gene            161..760
                     /locus_tag="VN034_09420"
     CDS             161..760
                     /locus_tag="VN034_09420"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWV60862.1"
                     /translation="MAPLKLALAAIALCWTSSAHADPVARWQPHIAEASARFGVPSDW
                     IERVMRAESGGRTTLGGRPITSRAGAMGLMQLMPGTWAMMRARLRLGTDPHDPRDNIL
                     AGTAYLRMMYDRFGHPGMFAAYNAGPGRYAAWLAGRARLPGETVDYLATVTGSDLPSR
                     TPPKLPPATSRQAAGKTAKMARIPAQEPDAIFFIRRPGS"
     gene            978..1337
                     /locus_tag="VN034_09425"
     CDS             978..1337
                     /locus_tag="VN034_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60863.1"
                     /translation="MGVLQNMDVASDRVFAALVAGLELEFGRGAGAALAQRFLDAEEV
                     DFHWDARVQERWIGAYEGCEDGDVELDRIAILGRLAGDYFVATMIVDGDGKVQGMMGR
                     YSFGCEIEARKAFLAAS"
     gene            1642..3621
                     /gene="rlxS"
                     /locus_tag="VN034_09430"
     CDS             1642..3621
                     /gene="rlxS"
                     /locus_tag="VN034_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830511.1"
                     /note="I built this because a sul1 chimera in AMR looks
                     like the C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease RlxS"
                     /protein_id="HWV60864.1"
                     /translation="MSDEKVFEPRLGRIGARGKAPGKKYLHRILAAANLAGAALGQSK
                     RKARFDGSRIGRGAGVGRVLGVRDEFAAFRRRRVIVKSRIVKLAGKGAAAARAHLRYV
                     ERDGTTRDGGRGRLYDAESDAVDGKSFLARGEDDRHHFRFIVSPEDGAEYDDLKSPTR
                     RLMAQMEADLGTALDWVAVDHFNTGHPHTHIIVRGKDDRGKDLIIAREYITGGMRERA
                     AELIDLDLGPRSDDAVRQRLRSEIEQDRLTGIDRALLREAETDILVGSAARGPFDQTL
                     RIGRLRKLERLGLAEPMGATHWRLVPGMAETLRRMGERGDIIRTMQRAFAEAGRAPAL
                     ADQAIYDPAAASQPIVGRIRKRGLSDEVSDRHYLMIDAADGRTHYVDIGKGENLDVHS
                     AGAVVRITPTILGTRDSDRTVAEVAAANRGRYNVDLHLRHDPAASEAFAETHVRRLEA
                     IRRRTGGVTREADGSWIIAADHLDRAAAYEAARAKDRPVVIETLSIRPIEHLVDIDAA
                     TWLDRELVAADPVPLRDAGFGRDARVAQARRRQWLVAQGLAWESDGRFETSGDVVTRL
                     RQREVARVGARLSKELGRPYTRVESGDRVSGILGRRVDLVSGRYALIERARDFTLVPW
                     RSVLDRQLGKSVTGIVRGEGMSWTFGRGRGGPNIS"
     gene            complement(3844..5310)
                     /locus_tag="VN034_09435"
     CDS             complement(3844..5310)
                     /locus_tag="VN034_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60865.1"
                     /translation="MSEWQIKPGDFKRYPHFDQVLKPDEIMALVTDPVRVAQNTFFPL
                     LRYTKKWQPFRPDKGSGKPRPKPKDRPIRYASRRDAYIFAYYRRLLSGPYEAELERLG
                     ISHCPVAYRRIPISAENPRGKCNIQFARDAFDTIRELGSCCAIAVDISSYFESLDHEK
                     LKALWCRLLGTDRLPADHFAVFKNITKYRVVDRVDAYTRLGFYGVKGHTSGGHPINGY
                     LVPYKDVPTQLCSPHDFRQKILGENGQYKSLVDPNGKPHGIPQGAPISDLLANLYLID
                     FDVEMNVLAESFGGTYVRYSDDILLILPVPVDQARAIMDSLPARIGKYGDELVIKPSK
                     SSLVQYVRDGSRQRCTLIAGKGRNGLEYLGFRYDGQNILLRDSTMSNLYRKVASVARG
                     HAMATVKRYPGKPPSELRQMFNFEQFTKRFGRVEGFEPSSSTKTWTFWTYVVRSVEEF
                     GPAGLKIRGQVSRLRRRARHRVDEEIDHALAKRAKQNP"
     gene            5863..6174
                     /locus_tag="VN034_09440"
     CDS             5863..6174
                     /locus_tag="VN034_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWV60866.1"
                     /translation="MVWMARQSSKLKSGRDPEEVAASFSLESSQRLFAIGTFDDGVTV
                     LSQQKRALNLAWALIESRKIPTRPEEEAISIAIVGAGFAGLTFASALLQKNCRCDITI
                     F"
     gene            6223..>6817
                     /locus_tag="VN034_09445"
     CDS             6223..>6817
                     /locus_tag="VN034_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60867.1"
                     /translation="MHPHIYDWPEEGSEAAAAMLPVLNWTAARASDVVVQALSGWKSI
                     VKDHGSALRLYCNTRHLQVQSPAANKVLVEWVGDQRAAWDGNVLGEDRSAKGQSQRFD
                     LVVLAVGFGLEIGQSSYWRNEVYGQPSLSQPRRSYLISGQGDGAMIDLLRLKVSQFRQ
                     DRILTELFDDKPKLITRLRAIRREFLDGGPDFPLFAKL"
BASE COUNT         1282 a         2013 c         2161 g         1361 t
ORIGIN      
        1 tcttgcgcga tcgcgccggg cgaccgatgc cgcgctggcg gggttgcagg cggctccgag
       61 acgatgccgt gctgctcctg atggaggccc cggcgtcgtt cgacgggcgc tatttcgggc
      121 cgaccgcgcg ctgcgacatc atcggcaagg cgacgccgct atggctccgc tgaagcttgc
      181 gctcgccgcg atcgcgctat gctggacctc gtccgcgcac gccgatccgg tcgcgcggtg
      241 gcagccgcat atcgccgaag cgtcggcccg gttcggggtt ccgtccgact ggatcgagcg
      301 cgtgatgcgc gccgaaagcg gggggcggac gacgctcggc ggccgtccga tcaccagccg
      361 cgcgggcgcc atgggcctca tgcaattgat gccggggacg tgggcgatga tgcgcgcccg
      421 gctgcgcctt ggcaccgatc cccatgatcc gcgcgacaat atcctcgcgg gaacggccta
      481 tctccggatg atgtacgatc gtttcggcca cccgggaatg ttcgccgcgt acaacgcggg
      541 gccgggccga tatgcggcgt ggcttgcggg gcgcgcgcga cttccgggtg aaaccgtcga
      601 ctatctcgcc acggtgaccg gctcggacct gccctcgcga acgccgccga aattgccccc
      661 ggccacgtcc cgccaagccg ccgggaagac agcaaaaatg gcgcgcattc cggcgcagga
      721 accggatgcc atcttcttca tccgaaggcc tggatcatga cgcgggcgtg gtgaggggct
      781 cgtctcggca aggcccagtg tgacggccgc ccgagcgcct atcaaggccg gcggtacccc
      841 caatttgctt cgcaaatcgg ctcccccgcc gccgcttcgc ggcgccttcg ctgcgctccg
      901 gcggccctga cagccgctcc gccgcccgtc ggcgaggcta tgtcctcgaa cggttcgggg
      961 tgagaaagga gcatgtcatg ggcgttcttc agaacatgga tgtcgcgtcg gatcgcgtat
     1021 tcgcagcgct ggttgcgggt ctcgagctcg agttcgggag gggcgcgggc gcggcgctgg
     1081 cgcagcgttt tctcgatgcc gaggaggtcg attttcactg ggacgcgcgc gttcaggagc
     1141 gctggatcgg tgcctatgag ggctgcgagg acggcgatgt ggagctcgat cggatcgcga
     1201 tactggggcg gctggctggc gattatttcg tggcgacgat gatcgtcgat ggcgacggaa
     1261 aggtgcaggg catgatgggc agatactcgt tcggatgcga aatcgaagcc agaaaggcat
     1321 ttctggccgc atcgtagatt gccgcgcgga ggggcggcgc gggtagcgcc gctcctccct
     1381 ttttttgacc ccgcagcgac gaagcgacgg atttgcgccg tttttggaag gagggcgcgt
     1441 aaggcaagat taaacaatgg tgccggttct gcgcgccgtc cccataagct tttgtctgca
     1501 cgtcgctttt tcgtcgctcg cgcgcgattt tttggcaccg gcatctggcg cctggtgcca
     1561 gacgtgcggc agatcggctt gaatccatgc aaattttggc accggttttc aaatgcctgg
     1621 gaagcggtca tccttcccgt catgagcgac gagaaagtct tcgaaccccg gctgggccgg
     1681 atcggggcgc gcggcaaggc ccccgggaag aagtatctgc atcgcatcct tgccgccgcc
     1741 aatcttgccg gcgcggccct tggccaatcg aagcgcaagg cccggttcga cggcagtcgc
     1801 atcggccgcg gcgcgggggt cggacgcgtg ctcggcgtcc gcgatgagtt tgcggcattc
     1861 cgcagacgcc gcgtcatcgt gaaatcgcgc atcgtcaagc tggcggggaa gggcgccgcg
     1921 gcggcgcgcg cgcatctgcg ttatgtcgag cgcgacggga caacgcgcga tggcggacgc
     1981 ggccgtttat atgatgccga aagcgacgcg gtcgacggca agagctttct cgcgcgcggg
     2041 gaggacgacc gccaccactt ccggttcatc gtgtcgcccg aggacggcgc cgaatatgac
     2101 gacctcaaat cgccgacgcg gcgtttgatg gcgcagatgg aggctgatct gggaacggcg
     2161 ctcgactggg tggcggtcga ccatttcaac accggccatc cgcacacgca tatcatcgtt
     2221 cgcggcaagg atgaccgcgg caaggatctg atcatcgccc gcgaatatat tactggcggg
     2281 atgcgcgagc gcgcggccga actgatcgat ctcgatcttg gaccgcgatc ggacgacgcc
     2341 gtccgacagc gccttcgcag cgagatcgag caggaccggc tcacgggtat cgaccgcgca
     2401 cttctgcgcg aggcggaaac cgacattctc gtcggttcgg ccgcacgcgg tcccttcgat
     2461 cagacgttgc gcatcggtcg tctgcgcaag ctcgaacggc tcggacttgc cgagccgatg
     2521 ggcgcgacac actggcggct ggttcccggc atggccgaga ctttgcgccg catgggcgag
     2581 cgcggcgata tcatacgaac gatgcagcgg gcgttcgctg aagccggaag ggcgcccgcg
     2641 ctcgccgacc aggcgatcta tgatccggct gcggcgtcgc agcccatcgt cggccgcatc
     2701 cgcaaacggg gtttgtcgga cgaggtgagc gatcgccact atctgatgat cgacgctgcc
     2761 gacggccgca cccactatgt cgacatcggc aagggggaaa atctcgacgt gcattccgcc
     2821 ggcgcggtcg tccggatcac gccgaccatc ctcggcacac gtgattccga tcgtaccgtc
     2881 gccgaagtcg ccgcggccaa tcgagggcgg tataatgtcg atctgcatct tcgccatgat
     2941 ccggccgcga gcgaggcctt tgccgaaacg catgttcgcc ggctcgaggc gatccgccgc
     3001 cgaaccggcg gcgtcacccg cgaagccgac gggagctgga tcatcgctgc cgatcatctc
     3061 gatcgcgccg ccgcctatga agcggcgcgc gccaaggatc ggcccgtcgt catcgaaaca
     3121 ttgtcgatcc ggccgatcga acatcttgtc gatatcgatg ccgcgacatg gctcgaccgg
     3181 gagcttgtcg ccgccgaccc cgtgccgctt cgcgatgcgg gattcgggcg cgatgcccgc
     3241 gtggcgcagg cccgacgtcg gcagtggctt gtcgcgcagg ggcttgcatg ggaaagtgac
     3301 gggcgctttg aaacgagcgg cgacgtggtg acgaggcttc ggcaacgcga agtcgcgcgc
     3361 gtcggagcgc ggctttccaa agagctcgga cgtccataca cgcgggtgga gtcgggggac
     3421 cgggtgagcg ggatcttagg ccggcgcgtc gatctggtga gcgggcgtta cgcgctcatc
     3481 gagcgcgcac gcgatttcac gctggtgcct tggcggtcgg ttctcgatcg ccagttggga
     3541 aagagcgtca cgggcattgt ccgcggggaa ggcatgagct ggacgttcgg gcgcgggcga
     3601 ggcggcccga atatttcata gctgtgccaa atgcgacacg gccggttcgc gcctcatctc
     3661 ggactttccc ggcccttggg aaatcagcct gaaagcggac cttaggttgc gctgaaaatt
     3721 caactcttcg ccccaccgga cggccgtctg ttgcgaagat ggggaggagg gctctcggag
     3781 cagttggtga tgcatgtcca tcagctgttc gtgcagtcgc gctcgcgacc ctgagctccc
     3841 gctttacgga ttctgttttg ccctctttgc cagcgcgtga tcgatctctt catccaccct
     3901 atgcctagcc ctgcggcgaa ggcggctgac ctgaccgcga attttcaagc ccgcagggcc
     3961 aaactcctcc acagaacgca ccacatatgt ccaaaaggtc catgtcttgg ttgatgagct
     4021 gggctcaaaa ccttcgaccc gaccgaaacg cttagtgaac tgctcaaaat tgaacatctg
     4081 gcggagttcg gaggggggct tgcccggata gcgcttcacc gtggccattg catggccgcg
     4141 agcaactgat gctaccttac gataaaggtt cgacatcgtt gaatcgcgaa gcaggatgtt
     4201 ttggccatca tatcgaaagc cgagatactc gaggccattt ctgcctttcc cggcgatcaa
     4261 ggtgcagcgc tgcctggatc catctcgaac atattggacc agagatgatt tcgacggctt
     4321 gatgacgagc tcgtcaccat atttgccgat ccgggcaggc aagctgtcca ttatggcgcg
     4381 ggcctgatcg acaggcactg gcaggatgag gaggatgtcg tccgaataac ggacgtaggt
     4441 tcccccaaaa ctctccgcga gcacgttcat ctcgacatcg aaatctatca ggtagaggtt
     4501 agccagcaga tccgaaattg gcgcgccctg tgggataccg tgaggtttgc cgttgggatc
     4561 aacgagcgac ttgtactggc cattttcccc aaggatcttc tgtcggaaat catgcgggga
     4621 acagagttgg gtcggcacat ccttgtaggg cacgaggtag ccgttgattg gatggccacc
     4681 actagtgtga cctttgacgc cgtagaagcc caaacgggtg tatgcatcta cgcgatccac
     4741 cactcgatat ttcgtgatgt ttttgaacac agcgaaatga tcggcaggga gccgatcagt
     4801 gccgagcagg cgacaccaaa gcgctttcaa cttctcgtga tcgagggatt caaaatagct
     4861 gctaatatcg acggctatag cgcagcagct tcccagttct ctgatcgtgt cgaaggcatc
     4921 gcgcgcaaac tgaatgttgc acttacctct cggattctct gcgctgatgg gaatacgcct
     4981 gtaagcgaca ggacagtggc taatgcccag ccgctctagc tcagcctcgt aggggccgct
     5041 caacagacgt cgataatacg cgaaaatgta cgcatccctt cgggacgcat atcgaattgg
     5101 ccgatccttg ggcttcggac gaggcttgcc acttccctta tccgggcgaa agggctgcca
     5161 cttctttgtg tatcgcagca acggaaagaa cgtgttttgc gcaacgcgaa caggatctgt
     5221 cacgagggcc atgatttcgt cgggctttag aacctgatcg aaatgggggt aacgcttaaa
     5281 gtcacccggc ttaatttgcc attcgctcat gttgcctccc ccagaaacga gagggggcgg
     5341 cagcaaccgg ttccatccgg ttatcactac cgccccgcac cgttggctac gagctgggtc
     5401 gccccggctc tatctggaat tcacttcgac aaagtcgagt atgctgtcgc cgtcgcggct
     5461 atcgcgcatt tcgcgatggt cggttggcgg acaatacgtt cttcggcttt caacggagtt
     5521 gctcaaatga ccatccgctt catgcttcgg ttcgcattca tcctttacag tcataacacg
     5581 tgactctacc ccccgagtga gtagatacca aacatgttgg aacgcggtta acgaacatca
     5641 agaccttgcg gcagaattat ttcgtggcga tgccgcgggg agatattatg ccgctaattt
     5701 tgacgccgtg aagatcgaat gtttctcctc ggttatcgaa tctcgtggga tgccacgttg
     5761 attagctcaa ggatgttcga ggcgattgcc gtcgttgccg ggcggggtcc tcgctcaaat
     5821 atatgctgat tggggctttg cgaagtccgt tgcgaagggc aaatggtctg gatggcgaga
     5881 cagtcttcta agctgaaatc aggccgcgac cctgaagagg tcgcagctag cttcagcctc
     5941 gagtcatcgc agcggctatt cgcgatcggc acctttgacg atggcgtcac tgtactcagc
     6001 cagcagaagc gcgctctgaa cctcgcatgg gccctaatag agtcgcgcaa aataccgacc
     6061 agacccgaag aagaggcgat atccatcgct atcgtcggcg ccgggtttgc cgggcttact
     6121 ttcgcgtccg cgctccttca gaagaattgt cgctgcgaca ttacgatctt ctaagagcgc
     6181 gacacacttc ttccgctcca gcaaggaagc gacacaagat ggttgcatcc gcatatctac
     6241 gattggccgg aggaaggaag cgaagctgcg gcggcaatgc tgcccgtcct gaattggact
     6301 gccgcgcgtg cctctgacgt tgtcgttcaa gcgttaagcg gctggaagtc tatcgtaaaa
     6361 gatcatggct ctgctctaag actgtattgc aacacccgtc accttcaggt gcagtcgcca
     6421 gcggccaaca aagtcctagt ggagtgggta ggcgatcagc gggcggcatg ggacgggaac
     6481 gtccttggag aagatcgcag cgccaaagga caatctcaac ggtttgacct tgtcgtcctt
     6541 gctgtcggct tcgggcttga aattggccag tcatcgtact ggcgtaacga ggtctacggg
     6601 caaccaagcc ttagccagcc taggcgttcc tatctcatct cgggtcaggg cgacggggca
     6661 atgatagatt tgctccggct taaggtctcg cagtttcgcc aagacagaat tctcacggag
     6721 ctttttgatg ataagccgaa gctcatcacg cggctacggg ctataaggcg cgaatttctg
     6781 gatggcggac ctgacttccc tctgttcgcg aagctag
//
LOCUS       DATMSU010000350        11301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_72389, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000350 DATMSU010000000
VERSION     DATMSU010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72389"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VN034_09450"
     CDS             <1..609
                     /locus_tag="VN034_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60868.1"
                     /translation="YVALFDGDFWSSSFSVVIRGNKLRVHHARQLESVHNSEIPSLNE
                     KALVELENMVGIHNLLVQTNPDLKLFDDQLRNIDGNSSGDPRATLRRMIATAETAAAL
                     DENAQSVLKSLPEYSSQDQESVDLKIHLVIENLTRSVMKYLWERKKVITIGAIGFPPA
                     LYAIGKWALANEGILLGYFAEGSNMSIVLNTIMRWLHTLPLA"
     gene            622..930
                     /locus_tag="VN034_09455"
     CDS             622..930
                     /locus_tag="VN034_09455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HWV60869.1"
                     /translation="MILITGHVILTPEHRDRMIALGAEHSARSRSEPGCLAHNCHIDV
                     ENPDRLMFVEEWESVDAVRAHFALPASRAFVAEMRALSPEPPAIRIYAAEDITATLMG
                     "
     gene            complement(1050..3944)
                     /gene="uvrA"
                     /locus_tag="VN034_09460"
     CDS             complement(1050..3944)
                     /gene="uvrA"
                     /locus_tag="VN034_09460"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWV60870.1"
                     /translation="MSLTHISVRGAREHNLKGVNVDLPRDALIVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLEMMQKPDVEHIDGLSPAISIEQKTTSRNPRSTVAT
                     VTEIYDYMRLLWARVGIPYSPTTGEPISAQTVSQMVDRVMELPEGTRAYLLAPVVRGR
                     KGEYKKELAQWQKDGFTRVRIDGELYEIGDAPALDKKYKHDIEVVVDRIAVREGLGTR
                     LADSFETALKLAEGLAYVDLADGPVPGREEEDTGGAMKGAGIPANRLIFSEKFACPVS
                     GFTIAEIEPRLFSFNAPQGACPACDGLGERQEFDPDLVVPNHALSLKKGAVVPWAKSN
                     PPSPYYMQVLESLGKAYGFDLTTPWQDLPGEVQLIILYGSGGKPVELTFKDGKRSYTT
                     HKAFEGVIGNLNRRLLQTESAWMREELSKYQAPHPCETCHGARLRPEPLAVKIAGENI
                     SMSAQRSVADALGWFSTLDEKLNDTQKQIAKAILKEINERLGFLNNVGLDYLNLNRTS
                     GTLSGGESQRIRLASQIGSGLSGVLYVLDEPSIGLHQRDNDRLLATLKRLRDLGNTVI
                     VVEHDEDAIRHADYVVDMGPGAGLHGGEIVAEGTLEQVLANTKSLTAAYLTGAKKIEV
                     PKHRRPGNGFDLVLKGARANNLQNVTAKIPLGTFTCVTGLSGSGKSSLIIDTLYASAA
                     RVLNGARMVAGPHDSLTGLEHCDKVIDIDQSPIGRTPRSNPATYTGAFTIIRDWFAGL
                     PESQARGYKPGRFSFNVKGGRCETCTGDGLIKIEMHFLPDVYVTCETCHGKRYNRETL
                     EVKFKGMSIADVLDMTVEDAADFFKAVPAIRDKMAMLVRVGLGYIKVGQQATTLSGGE
                     AQRVKLAKELSRRSTGQTLYILDEPTTGLHFEDVRKLLEVLQALVDQGNSVVVIEHNL
                     DVIKTADWIVDLGPEGGVKGGEIVAAGTPEQVVKAKRSFTGQYLAPLLGK"
     gene            complement(4084..6741)
                     /locus_tag="VN034_09465"
     CDS             complement(4084..6741)
                     /locus_tag="VN034_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60871.1"
                     /translation="MKNRFAVASSSLVLAFALSACGGGGGGGVNSTPPPPTPTPTPTP
                     TPTPTPTPTPTPTPTTANADLVNLASSERFTNDAVTGTATVPLDGSPGTTNAARATVT
                     IEYSATERSYTVTTAGRSQTFRPADIDAARSSAAATVYVRTNGATADVLTLTEPGTSG
                     RLTYQYVAGGFWERTVNGTTSTSGSVDGFAYGVETPDVALPRSGTANYGIDLIGVMGY
                     ANSAPSPLAGRGTLYVDFGKGSIVITGEADGGTTFNGTATLASTSNNFSGRFEYYSLG
                     SMTGPIDGRFFGPGAQEVGAAWHVKNGGNAAAGVILGRKNPDAPANSDFGNIANSQFF
                     ENSGVILKATYAKGSNNSSDKSTQASDFLVHYDAGYKSYTIVAGDKAEFFGPDRVPDN
                     AAPGSHWTKGIGTTRSALQFLNGGTHVTGARYVFTDGDATNSFVTSNFFIYGFPTANA
                     DIVRSGTSFFKSSIAGSMTDQGYDSLLDITGPGVIQVSFATGAIDIRGAFDIVHRPIT
                     GPRTVGNGSYTGTAQLASSANSFTGSIELAANDTYLGNLSGRFYGPTGKELGAVFKAS
                     DSSGDVAAGTITASLDPSANASTKALADLTETTVFIGSKAQAGIAVDQPAGRQFSLGA
                     PSVSRTEQEPRITYDPQTGSYIVREFGGDYRDQLLETLRFGPADRDTAASNAEFDIFN
                     VSSGNTTHRISVLRPGAGNPAIQLTYASFIGVVSKTIEGAPINRDTTRYSYFAFGTPT
                     ADTLMPRSGTGTYNGLATGYGTVRGIGFAEYGISGTSKLSVNFRSLATQLNLTLAGRN
                     LADGTNFNFGDFVFNGALGYSLVDGQRLNQVSMGLKRDTLPPGSSGDAFGHLYGAAGQ
                     EHVTPFGIQIRSADGSETVIGGVAYGKRD"
     gene            complement(6786..8108)
                     /locus_tag="VN034_09470"
     CDS             complement(6786..8108)
                     /locus_tag="VN034_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HWV60872.1"
                     /translation="MSAALAAAAFHGLPAHAQENAAGNAAQPVRVSPEQLFAIADQAR
                     SARDFATAETAWRALATNPDIELRTEARFRLAMMLADDLGKYREAAVLFREILDEKPG
                     AARVRIELARMQAMLGDLRAAERELRSAQAAGLPPEVEQQIRFYAAALSARKPFGGSV
                     SIAFAPDSNINRATRSDTLGTIIGDFTLDEDAKARSGLGLALRGQAYFRTGIDKRSQL
                     LVRASTYANLYRAKQFDDIALSIQAGPEYASGKDRINISGEATWRWYGLDPYSFSAGV
                     GATWQHPMGDKAQLRIDGRVADVNNRRNPLQDATNLLLAASYDRAFSAKFGGGLQVSG
                     NREAARDPGYSTASGGVTVYLFRELGQTTLTASAGYSRLEADRRLFLYPRRRVDDRLQ
                     ASLGGHFRFLKIGSFAPTVKVGWERNKSTVEIWDYSRISAEFGITSAF"
     gene            8347..10425
                     /locus_tag="VN034_09475"
     CDS             8347..10425
                     /locus_tag="VN034_09475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HWV60873.1"
                     /translation="MKTFLAGAAGLAIAAALAGVPVQAKDAQPSVSLTTAADEAAGDT
                     EEGSLKGLTFGKWGVDLGARDTSVKPGDDFDKYANGGWFARTEIPADQASAGVDYDVY
                     NLTQRQLRAVVSGAPATSQVGGLYQSFMDEARVEALGTKPLMADIAAVAAIKDKSVMA
                     RFMGGTQGSFGSTIVSGGPYADTADPTVNVLWLGQAGIGLPERDYYLNDSFKPQRDAY
                     RAYIARTMKMAGNADPEKAADAIMAFETEIAKVSWAIADRRDIGKINNPMSSDELAAY
                     APGLDWKAWFEGAGIAPQKRIIVNEKTAVRDIAALYAKTPLDTIKLWQQFHVADNAAP
                     YLDKAFVDSRFEYTKALSGVGELRPRWKRGLTLVDGSLGELVGETYSKQYFPPSAKAK
                     METLVANLKLAMGDRIRANSWMAPATKDAALAKLAKMDVMVGYPDKWRDYSGLKIDAA
                     DLYGNVKRSSAFEYAYALSDLNKPVDRKKWAMNPQTVNAYNGGLENKIVFPAGILQAP
                     YFSESVDDAVNYGAIGAVIGHEITHGFDDQGRKIDAEGAVRDWWTAEDAARFDAQAKA
                     FGAQYATYEAAPGAFINPDLTMGENIADLAGLEVAYDAYHRSLNGKQAPVIDGLTGDQ
                     RFFLAFAQAWRDKAREDTIKQQVASDPHSPARWRIIGPVRNVDAWYKAFGVDAGAQYY
                     LKPEARTKIW"
     gene            complement(10442..>11301)
                     /locus_tag="VN034_09480"
     CDS             complement(10442..>11301)
                     /locus_tag="VN034_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HWV60874.1"
                     /translation="FDGGTALRWAIAAAIVASFLISAATGLQKGIAGLSVLNTWIFFA
                     IIAFVLLAGPTASMLDLALRGVADYFATFVPRNLGLDVDTGWHRQWTVFNWANWFAWA
                     PVTALFLGRLAVGYSVRAFILYNLFLPSLFGAVWMTAIAGATIALDQQSGAGLYAILT
                     AQGPDPVLFRLFSALGGGPIVAGIVLFAIFLSYVAGADANVSAMSALSTQGITPERPE
                     APLWVQAVWGVTVGLVALVLVAGGGIDGIRMMSVLGGFPALFVITGAALSLTVMAVRG
                     RHEAAPARS"
BASE COUNT         2052 a         3441 c         3680 g         2128 t
ORIGIN      
        1 tacgttgctt tgtttgatgg cgatttttgg tctagctctt tttcagttgt catcaggggt
       61 aacaagcttc gggtccacca tgcccgtcag ctcgaatccg ttcataatag tgaaatacct
      121 tccttgaatg agaaggcgtt agtcgagctg gagaatatgg ttgggattca taatcttctg
      181 gtgcagacta acccggattt aaaattattc gacgaccaac ttcgtaatat tgacggaaat
      241 tcttctggag atccgagggc gacattacgt cgcatgattg caacggcaga aacggccgca
      301 gccctcgatg aaaatgctca gtcggtgctt aaaagcctcc ccgaatattc gagccaagac
      361 caagaatctg tggacctaaa gattcatctt gtgattgaaa atcttactcg ttctgtgatg
      421 aaatatttgt gggaaaggaa gaaagtaatt acaatcggag ctattggttt tcctcctgct
      481 ctttacgcga ttggaaaatg ggcgctcgcg aacgaaggaa tccttcttgg atattttgca
      541 gaaggatcaa acatgtcgat cgttctgaac actattatgc gatggcttca cactcttcct
      601 ttagcgtaaa atggggctgt aatgatcctc atcaccggcc acgtcatcct aacccccgag
      661 catcgcgacc gcatgatcgc gctcggtgcc gaacatagcg cgcggtcgcg ttccgaaccg
      721 gggtgtctgg cgcataattg ccatatcgat gtcgaaaacc ccgaccggct gatgttcgtc
      781 gaagagtggg agagcgtcga cgcggtgcgc gcccattttg ccctgcccgc atcgcgcgcc
      841 ttcgtcgccg aaatgcgcgc gctctcgccc gagccgcccg cgatccgcat ctacgccgcc
      901 gaggatatca ccgcgacgct gatggggtag ggccgctcgt ccatcaactc cgtcaccccg
      961 gacttgatcc ggggtccagc ttttcaccgt cgcttagctg gaccccggat caagtccggg
     1021 gtgatgaaga gggggcgggc gggggcgtcc tacttcccca gcagcggcgc cagatactgc
     1081 cccgtgaagc tgcgcttcgc cttcaccacc tgctcgggcg tgcccgcggc gacgatctcg
     1141 ccgcccttga ccccgccttc ggggccgaga tcgacgatcc agtcggcggt cttgatgaca
     1201 tccagattat gctcgatcac cacgacgctg ttgccctgat cgacgagcgc ctggagcact
     1261 tcgagcaatt tgcggacgtc ctcgaaatgc agtcccgtgg tcggctcgtc gaggatgtag
     1321 agcgtctgcc cggtcgaacg gcgcgagagt tccttcgcga gcttgacgcg ctgcgcctcg
     1381 ccgcccgaca gcgtcgtcgc ctgctgcccg accttgatat agccgagccc gacgcggacg
     1441 agcatcgcca tcttgtcgcg gatcgcgggc actgccttga agaagtccgc cgcatcctcg
     1501 accgtcatgt cgagcacgtc ggcgatgctc atgcccttga acttcacctc gagcgtttcg
     1561 cggttgtagc gtttgccgtg gcacgtctcg cacgtcacat agacgtcggg caggaagtgc
     1621 atctcgatct tgatcagccc gtcgccggtg caggtctcac accgccctcc cttgacgttg
     1681 aagctgaaac gcccgggctt gtagccgcgc gcctggcttt cgggcaaccc cgcgaaccag
     1741 tcgcggatga tcgtgaaggc gccggtgtac gtcgcggggt tcgagcgcgg ggtgcggccg
     1801 atcggcgact ggtcgatgtc gatcaccttg tcgcaatgtt cgaggccggt gaggctgtcg
     1861 tgcggccccg cgaccatccg tgcgccgttg agcacgcgcg ctgcgctggc atagagcgtg
     1921 tcgatgatca ggctcgactt gccgctgccc gacaggccgg tgacgcaggt gaaggtgccg
     1981 agcgggatct tcgcggtgac gttctggagg ttgttcgcgc gcgcgccctt gagcacgagg
     2041 tcgaagccgt tgccgggacg gcggtgcttc ggaacctcga tcttcttcgc gccggtcaga
     2101 tacgccgcgg tcaggctctt cgtgttcgcg agcacctgtt ccagcgtgcc ctcggcgacg
     2161 atctcgccgc cgtggagccc cgcgccgggg cccatgtcga cgacataatc ggcgtggcgg
     2221 atcgcatcct cgtcatgctc gacgacgatc accgtgttgc cgaggtcgcg gaggcgcttc
     2281 aaggtcgcga gcagccggtc gttgtcgcgc tggtggaggc cgatgctcgg ttcgtcgaga
     2341 acgtagagca cgcccgacag ccccgagccg atttgcgagg cgaggcgaat gcgctggctc
     2401 tcgccgccgc tcaaggtgcc cgaggtgcgg ttgaggttga gatagtcgag cccgacattg
     2461 ttgaggaagc cgaggcgttc gttaatctct ttgaggatcg ccttggcaat ctgcttctgc
     2521 gtgtcgttca gcttctcgtc gagcgtactg aaccagccga gcgcgtcggc gaccgaccgc
     2581 tgcgccgaca tgctgatatt ctcgcccgca atcttcaccg cgagcggttc ggggcgcagc
     2641 cgcgcgccgt ggcacgtctc gcacggatgc ggcgcctgat atttgctcag ctcctcgcgc
     2701 atccatgcgc tttcggtctg gagcaggcgg cggttgaggt tgccgatcac gccctcgaac
     2761 gccttgtgcg tcgtgtagct gcgtttgccg tccttgaagg tcagttcgac gggcttgccg
     2821 ccgctgccat agaggatgat cagctgcacc tcgccgggca aatcctgcca cggggtcgtc
     2881 agatcgaaac cataggcttt gccgaggctt tcgagcacct gcatataata gggcgagggc
     2941 gggttcgatt tcgcccacgg caccaccgcg ccctttttca aggacagcgc atggttcggg
     3001 acgacgaggt cgggatcgaa ctcctgccgc tcgccgagcc catcgcacgc cgggcaggca
     3061 ccctgcggtg cgttgaagct gaacagccgc ggctcgatct cggcgatcgt gaagcccgac
     3121 acggggcagg cgaatttctc ggagaagatc agccggttcg cggggatgcc cgcgcccttc
     3181 atcgcgccgc cggtgtcttc ctcttcgcgc cccggcaccg gaccgtcggc gaggtcgaca
     3241 taggcgagcc cctcggcgag cttgagtgcg gtctcgaagc tgtcggcgag ccgcgttccg
     3301 aggccctcgc ggacggcaat gcgatcgacg acgacctcga tgtcatgctt gtatttcttg
     3361 tcgagcgccg gcgcgtcgcc gatctcgtat aactcgccgt cgatgcggac gcgcgtgaag
     3421 ccgtccttct gccactgcgc gagttccttc ttatactcgc ccttgcgccc gcgcacgacg
     3481 ggggcgagca gataggcgcg cgttccttcg ggcaattcca tcacgcggtc gaccatctgg
     3541 ctgaccgtct gcgcgctgat cggctcgccg gtcgtcggcg aatagggaat gccgacgcgc
     3601 gcccacagca ggcgcatata gtcgtagatt tcggtgaccg tcgcgacggt cgagcgcggg
     3661 ttccggctcg tcgtcttctg ctcgatgctg atcgccggcg acagcccatc gatatgctcg
     3721 acatcgggct tctgcatcat ctcgaggaac tggcgcgcat aggcggacag gctctcgaca
     3781 tagcgccttt ggccttcggc atagatggta tcgaaggcga gcgacgattt gccgctcccc
     3841 gacagaccgg tgatgacgat cagcgcatcg cgcggcaggt cgacgttcac gcccttgagg
     3901 ttatgctcgc gcgcgccgcg cacggagatg tgggtgagac tcatgggaga ggtttgttcc
     3961 agatttgttc taagggcgca agaggccgcg aacggactcc gccgcgtcgc ccgaagatag
     4021 gaacgcaaag gctcggccgc aacggcgccg cgcaaggacg ccgttgcgat ccgggcgctt
     4081 cgatcagtcg cgcttcccgt aagcaacgcc gccgataacg gtctccgacc catcagcgct
     4141 gcggatctgt atgccgaacg gcgtcacatg ctcctgtccg gcggcgccat agagatgccc
     4201 aaatgcgtcg ccgctcgaac ccggaggcag cgtgtcgcgt ttgagcccca tcgatacctg
     4261 attcaggcgc tgtccatcga cgagcgaata accgagcgcg ccattgaaga cgaaatcgcc
     4321 gaagttgaaa ttcgtgccgt cggcgagatt gcggccggcc agtgtcaggt tgagctgggt
     4381 ggcgagcgac ctgaagttga ccgacagctt gctggtgccg ctgattccat attcggcaaa
     4441 gccaatacca cgaaccgttc catagccggt cgcgaggccg ttgtaggtgc cggttcccga
     4501 ccgcggcatg agcgtatcgg ccgtcggtgt gccaaaggcg aaatagctgt agcgggtggt
     4561 gtcgcgattg atcggcgctc cttctatggt ttttgatacg acgccaatga agcttgcata
     4621 ggtaagctgg atagccggat tgccggcacc cgggcgaaga acgctgatgc gatgcgtcgt
     4681 attgccgctc gatacgttga agatatcgaa ttcggcattg ctcgcggctg tgtcgcgatc
     4741 ggcggggccg aagcggagcg tctcgaggag ctggtcccga tagtcgccgc cgaactcgcg
     4801 gacgatatag ctgcccgttt gcgggtcata ggtgatgcgg ggctcttgtt cggtcctcga
     4861 aaccgacggc gcgccaagcg aaaattgccg gccggccggt tggtcgaccg cgatccccgc
     4921 ctgcgccttg ctgcctatga agaccgtcgt ttcggtgagg tcggcgagag ctttcgtgct
     4981 cgcattggcg gaagggtcga gcgatgcggt gatcgtgcct gcagcgacgt cgccgctgct
     5041 gtcgcttgcc ttgaacaccg cgccgagctc tttccccgtg gggccgtaaa agcggcccga
     5101 caggttgccg agataggtgt cgttcgcggc gagttcgatc gaacctgtaa agctgttggc
     5161 gctgctcgcg agctgcgcag tgccggtgta ggaaccattg ccgacggtgc gcgggcccgt
     5221 aatcggccgg tggacgatgt cgaacgcccc gcgaatatcg atagcgccgg ttgcgaaact
     5281 gacctggatc acgccgggac cggtgatgtc gagcaggctg tcatatccct gatcggtcat
     5341 cgagccagcg atgctgcttt tgaaaaagct tgttcccgaa cggacgatgt cggcgttcgc
     5401 ggtggggaag ccgtagatga agaaattcga tgtcacgaac gaattggtcg catcgccatc
     5461 ggtaaagacg tagcgcgcgc ccgtcacatg cgtgccgccg ttgagaaact gcagcgcgct
     5521 tcgcgtcgtc ccgattcctt tggtccaatg agagccgggc gcggcattgt cgggcacccg
     5581 gtcggggccg aaaaactcgg ccttatcgcc cgctacgatc gtgtagctct tgtagcccgc
     5641 gtcataatgc acgaggaaat cggacgcctg cgtcgatttg tcgctgctgt tgttcgaacc
     5701 tttggcgtag gtcgccttca ggatgacccc cgaattttcg aagaactggc tgttcgctat
     5761 attgccgaaa tcgctgttgg cgggcgcgtc ggggtttttg cggccgagta tcacgcctgc
     5821 ggcggcgttt ccgccattct ttacatgcca ggctgcgccg acctcctgcg cgccggggcc
     5881 aaaaaagcgt ccgtcgatcg gcccggtcat cgacccgagc gagtaatatt cgaaccgccc
     5941 ggagaaattg ttgcttgtgc tggcgagcgt ggccgtgccg ttgaaggtcg tccccccgtc
     6001 ggcttcgccc gtaatgacga tcgagccttt gccaaaatcg acgtaaagcg tcccccgtcc
     6061 ggcgagcggc gagggcgccg aattggcgta tcccatcacc ccgatcagat cgatgccata
     6121 attggctgtc cccgaacggg gcagggcgac atcaggggtt tcgacgccgt aggcaaagcc
     6181 atcgaccgat ccgctggtcg atgttgttcc attcaccgtg cgttcccaga agcctcccgc
     6241 gacatattga taggtcaggc ggccgctggt tcccggctcg gtcagggtga ggacatcggc
     6301 cgtcgcgcca ttggtcctga catagacggt tgcggcggca gagcttcggg ccgcatcgat
     6361 atcggcgggg cggaatgtct ggctgcggcc tgcggtcgtc accgtatagc tccgctcggt
     6421 cgccgaatat tcgatcgtca ccgtcgcacg ggccgcattg gtggtccctg gcgacccgtc
     6481 gagcggcacg gtcgctgtgc cggttaccgc atcgttggtg aagcgctcgc tgctcgcaag
     6541 attgaccaga tcggcgttgg ctgtcgtcgg ggtaggtgta ggcgtaggcg taggtgtcgg
     6601 ggtaggcgtc ggagtgggcg taggcgtagg cgtaggaggc gggggcgtgc tgttgacgcc
     6661 gccaccgcca ccgccgccgc atgcggacag agcaaaggcc agaaccagcg acgacgacgc
     6721 gaccgcgaag cgatttttca tttgatcagg cccccctgtt ccccattttt atccggtata
     6781 tcgctttaaa aagcggaggt tatgccgaat tctgcactga tacgcgaata gtcccaaatc
     6841 tcaacggtcg atttatttcg ttcccagccg actttgactg ttggcgcaaa gctgccgatc
     6901 ttgaggaagc gaaaatggcc gccaagcgaa gcttgcagtc ggtcgtcgac ccgtcggcgc
     6961 ggatagagaa agagccgccg gtcggcttca agccgactat agcccgcgct cgcggtcagc
     7021 gtcgtttggc cgagctcgcg gaagaggtag accgtaacgc cgcccgatgc cgtcgagtat
     7081 ccggggtcgc gggcggcctc gcggttcccg ctgacttgca ggccgccgcc gaactttgcc
     7141 gaaaaggcgc ggtcatagga tgccgcgagc agaaggttcg tcgcatcctg cagcggattg
     7201 cggcggttgt tcacatcggc aacccgaccg tcgatccgca actgcgcttt gtcgcccatc
     7261 ggatgctgcc atgtcgcccc gactcccgcc gagaagctgt agggatcgag gccgtaccag
     7321 cgccatgtcg cttcgcccga tatgttgatc cggtcctttc ccgacgcata ttcggggccc
     7381 gcctggatcg agagcgcgat atcgtcgaac tgtttggcgc gatagagatt ggcataggtc
     7441 gacgcgcgga cgagcaattg cgagcgcttg tcgatgccgg tgcggaaata ggcctgcccg
     7501 cgtagcgcga gccccaggcc cgaccgcgcc ttggcatcct cgtcgagagt gaaatcgccg
     7561 atgatcgtgc cgagggtgtc cgaccgggtg gcacggttga tattgctgtc gggtgcgaaa
     7621 gcgatggaaa ccgatccgcc gaacggcttt cgcgcgctga gcgcggcggc atagaagcgg
     7681 atctgctgtt cgacctcggg cggcaggccg gcggcctgcg ccgagcggag ctcgcgctcc
     7741 gccgcgcgca ggtcgccaag catcgcctgc atccgcgcaa gttcgatgcg gacgcgcgcg
     7801 gcgccgggct tttcgtcgag gatctcgcga aacagcaccg ccgcctcgcg atacttgcca
     7861 agatcgtcgg cgagcatcat tgccagccgg aagcgcgctt cggtgcgaag ttcgatatcg
     7921 ggattggttg ccagcgcgcg ccaggcggtt tcggcggttg cgaagtcgcg tgccgatcgc
     7981 gcctgatcgg cgatggcgaa cagctgctcg ggcgaaaccc ggacgggttg cgccgcattc
     8041 ccggccgcat tttcctgagc gtgggcaggg agaccatgaa aggccgcggc cgccaatgcc
     8101 gccgacatca atcgttgaat cgtccttgcc ccccagcgag acggcgcctg ccgtcgcttg
     8161 tcccttcgcc atataaatgt tccgcgtcaa tatcttcccc cgaccgaaag cgacaaatct
     8221 accaaagccg gattcctgtc gaccaatgcc cggcggttcg cttgaccgct tggcgtcacg
     8281 ggcggacaag tcgatcatga ccactgtcct gccgggacgg tgcggggaat atgaggagac
     8341 tggggaatga agacattttt ggcaggggcc gcaggccttg cgatcgccgc cgcgctggcc
     8401 ggcgtgccgg tgcaggcgaa agacgcgcag ccatccgtat cgctgaccac cgccgccgac
     8461 gaagcggcgg gggacaccga agagggatcg ctcaaggggc tgacgttcgg gaaatggggc
     8521 gtcgatttgg gcgcgcgcga cacgtcggtg aaaccgggcg acgatttcga caaatatgcc
     8581 aacggcggct ggttcgcccg gaccgagatt ccggccgacc aagcctcggc gggggtcgat
     8641 tacgacgtct ataatctgac tcaacgccag ctgcgcgcag tcgtttcggg cgcgccggcg
     8701 accagccagg tcggcggcct ttaccagagc ttcatggacg aggcgcgggt cgaggcgctg
     8761 ggcacaaaac cgctgatggc ggacatcgcc gcggtagccg cgatcaagga caagagcgtg
     8821 atggcgcgct tcatgggcgg aacgcagggc agcttcgggt cgacgatcgt cagcggcggt
     8881 ccctatgccg acaccgccga tccgacggtc aacgtattgt ggctgggtca ggcggggatc
     8941 ggcctgcccg agcgcgacta ttatctgaac gacagcttca agccgcagcg cgatgcctat
     9001 cgcgcctata tcgcacggac gatgaagatg gcgggcaacg ctgaccccga aaaggcggcc
     9061 gacgcgatca tggcgttcga gaccgagatc gcgaaggtca gctgggcgat cgccgaccgc
     9121 cgcgatatcg gcaagatcaa caacccgatg tcgtcggacg aactcgccgc ttatgcgccg
     9181 gggctcgatt ggaaggcctg gttcgagggc gccggcatcg cgccgcagaa acgcatcatc
     9241 gtcaacgaga agaccgcggt gcgcgatata gcggcgctct atgccaagac gccgctcgac
     9301 acgatcaagc tgtggcagca attccatgtc gccgacaatg cggcgcccta tctcgacaag
     9361 gcgttcgtcg acagccgctt cgaatatacg aaagcgctga gcggggtcgg cgaactgcgt
     9421 ccgcgctgga aacgcgggct gacgctcgtc gacggtagcc tcggcgaact ggtcggcgaa
     9481 acctattcga agcaatattt cccccccagc gccaaagcca agatggagac gctcgttgca
     9541 aatctgaagc tcgcgatggg cgatcgcatc cgggccaaca gctggatggc acccgccacg
     9601 aaggatgcgg cgctcgcgaa gctcgcgaag atggacgtca tggtcggcta tcccgacaaa
     9661 tggcgcgact attcggggct gaagatcgac gccgccgacc tttacggcaa tgtgaagcgc
     9721 agctcggcct tcgaatatgc ctatgcgctg tcggacctga acaagccggt cgatcgcaag
     9781 aaatgggcga tgaacccgca aacggtgaac gcctacaacg gcgggctgga aaacaagatc
     9841 gtgtttccgg ccggcattct gcaggcaccc tatttcagcg agagcgtcga cgatgcggtc
     9901 aactatggcg cgatcggcgc ggtgatcggc catgaaatca cccatggttt cgacgatcag
     9961 ggccgtaaga tcgacgctga gggcgcggtg cgcgactggt ggacggctga ggacgcggcg
    10021 cgcttcgatg cgcaggcgaa ggcgttcggc gcgcaatatg cgacttatga ggccgcgccg
    10081 ggcgccttca tcaatcccga tctgacgatg ggcgagaata tcgccgatct ggcgggactc
    10141 gaggtcgcct atgatgccta tcaccgctcg ctgaacggca agcaggcccc ggtaatcgat
    10201 ggcttgaccg gcgatcagcg cttcttcctc gcctttgcgc aggcgtggcg cgacaaggcg
    10261 cgcgaggata cgatcaagca gcaggtggcg agcgatccgc acagccccgc gcgttggcgt
    10321 atcatcggtc ctgttcgcaa cgtcgatgcg tggtacaagg cgttcggggt cgacgcgggc
    10381 gcccaatatt atctgaagcc cgaggcgcgc acgaaaatct ggtagtcctg agaccccttt
    10441 ctcaggaccg ggcgggggcg gcctcgtgtc gtccccgtac cgccatcacg gtgagcgaca
    10501 gcgccgcgcc ggtgatgacg aacagcgcgg gaaagccgcc gagtaccgac atcatgcgaa
    10561 tgccgtcgat gccgccgcct gccacgagca ccagcgcgac aaggccgacg gtcacgcccc
    10621 acacggcctg aacccacaag ggcgcctccg ggcgttcggg tgtgatgcct tgcgtcgaca
    10681 gcgcgctcat cgccgagacg ttggcgtcgg cgccggcgac ataagacagg aagatcgcga
    10741 acagcacgat tcccgcgacg atggggcctc cgccgagcgc gctgaacagg cggaacagca
    10801 cgggatcggg gccctgcgcg gtcaggatcg catagaggcc ggcgccgctc tgctgatcga
    10861 gtgcgatcgt cgcgccggcg atcgcggtca tccagaccgc gccgaacagc gagggcagga
    10921 acagattgta gaggatgaag gcgcggacgc tgtagccgac cgcgaggcgg ccgaggaaga
    10981 gtgcggtgac cggcgcccag gcgaaccagt tggcccagtt gaagaccgtc cattggcgat
    11041 gccagccggt gtcgacgtct aggccgaggt tgcgcggcac gaacgtagcg aaataatctg
    11101 cgacgccgcg gagcgcgagg tcgagcatcg aggcggtcgg gcctgcgagc aggacgaagg
    11161 cgataattgc gaagaaaatc caggtattta gcaccgaaag gccggcgatg cccttttgca
    11221 ggccggtcgc cgccgagatg aggaagctcg cgacgatcgc cgccgcgatc gcccagcgca
    11281 gcgcggtgcc gccgtcgaaa c
//
LOCUS       DATMSU010000351         5170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_53373, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000351 DATMSU010000000
VERSION     DATMSU010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53373"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VN034_09485"
     CDS             <1..230
                     /locus_tag="VN034_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60875.1"
                     /translation="AARGIVRTDPADRLWLTEAGRRTLVELAAAAKAAEEDALGAFDE
                     NETALLKHLLKHVIRSTDPGLPDPWAAPETP"
     gene            261..809
                     /locus_tag="VN034_09490"
     CDS             261..809
                     /locus_tag="VN034_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV60876.1"
                     /translation="MERTQTIAGRGPISQIAWLTEDLDRAIDQWRRLAGVGPWTVYRN
                     VTLDDLYRGAPAMIRIDVGLAWQGETQIELIAPHGTGPSPYHDAGGRVRTGMHHVAWL
                     VDDVATAATDATAAGLVPVFSAESGGGATRVAYFEAPDEPAILLELIEATPTIREGFA
                     AGIAAARDWDGGNPVTIFDFAA"
     gene            820..1533
                     /locus_tag="VN034_09495"
     CDS             820..1533
                     /locus_tag="VN034_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD domain-containing protein"
                     /protein_id="HWV60877.1"
                     /translation="MEKIIYALWKDPAEDDERFNARLLGDIAARLGELAHAVRVNVQD
                     DDVATGTSPRMTSTRPQMDAVIQLWLPSANDPERATVDAIIADAAPRHAAWLVSESTI
                     IANTQHPPAPGARTEGFSQIVFLGLPPRLSHDGWRDVWQRQHSAVAVDTQSNFEYVQN
                     LIIRPLSYGAPAYAAMVEECFPAAALTDEAVYFDAAGDPARLAENQRLMMESCARFID
                     FDRIDCIPTSQFDIRPFAR"
     gene            1560..2066
                     /locus_tag="VN034_09500"
     CDS             1560..2066
                     /locus_tag="VN034_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWV60878.1"
                     /translation="MRNVDESLTRLDPDSIVRSDRVEIDAPAAIVWEILVDLPRYGEW
                     NPFCIAAESTLEMGAPVHMTLQSYTEPGAVFPNCEYVCAFEPERLLSWELPHDDAWPY
                     PARRDQIIEALGDEFCAYHSTDAFLGPNGIHVMRFCGPWVKRAFDDTAAALKTRAEAL
                     HRERKTAS"
     gene            complement(2203..4563)
                     /locus_tag="VN034_09505"
     CDS             complement(2203..4563)
                     /locus_tag="VN034_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60879.1"
                     /translation="MKHRTYCRAQIAAIALIAPWVASTAHAQEAAPAAEETTTGGLDE
                     IIVTAQKRSESLQDTPLAISAINSEMIEQRGITSVAALGSAAPNLIITETPSATANPS
                     ISIRGIVDNDPILTADPAVGLYVDGVIVGRSAGALFDMMDLERVEVLRGPQGTLYGRN
                     TTGGAVNLISAKPSKDFGGRLQVGYGSFDEYMVKALIDTGELGDTGLALKLGYYHSQR
                     NGTVDNLLEPSDKRDPGARNTDAVRAAVRFDKGTGFTVDYVFDSSDRNGRANAFQLRA
                     IRPDILEYLNFSPAFGGTAPQVSQDRLSQIRLDRDGNAHDKVQGHSLTLNLEIGDATL
                     RSITGYRKWDNRSDSSDLDGNGGLVGFLVSPAILVPPNAFIPQGVGPIDLFSTRNERR
                     QRQFSQEFNLIGSIGDEFDYVLGAYYFRERATEINPQSFTLVLPSPVPIPIAPGVDLN
                     SFGVNLETLLSYRHVSRSMAAFSQLTWKPAALDSRLGITGGLRYTEDKKHLIQSAPVP
                     PAPRDLTRKFNRLNWLANISYDWSDDIMTYVRASTGYKAGGFNARAADNNGFNPEDLV
                     SYEVGIKSELFDRRLRLNLAAFHAIYDDLQVQQFLAGTGGASSTTVNAAKATYTGIEG
                     EILAQIADGFTVDASFGYTDRKYKNFTFVDPVTGVATDISDVAKFQYSAATTANVGAQ
                     YEAELGNFGKLVARADWNYRSKIYWHPVNPYNEEISDGGVGLLNARLSISDIAVGGAR
                     ASIAVWGKNLTKEDYLLSGIDFGSLGFAGVMYGDPRSFGVELGFKF"
     gene            complement(4765..>5170)
                     /locus_tag="VN034_09510"
     CDS             complement(4765..>5170)
                     /locus_tag="VN034_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWV60880.1"
                     /translation="CARRSGGARGSGGMPGAREGPRLMARVTYVQPDGSEKTCTNFEG
                     MAVMHLAIGNLVDGIDALCGGVRQCATCHVYVDDDWAARVGPPGDDEAEMLDALADVI
                     EVKPTSRLSCQIHITEELDGLRVHIPKEQPGI"
BASE COUNT          917 a         1688 c         1658 g          907 t
ORIGIN      
        1 tcgcggcgcg cggcatcgtc cggaccgatc ccgccgaccg gctctggttg accgaggccg
       61 gccgccgcac gctcgtcgaa ctcgccgccg ccgccaaggc ggccgaggag gatgcgctcg
      121 gcgccttcga cgagaatgaa acggcgctgc tcaagcatct gctcaagcac gtcatccgca
      181 gcaccgatcc ggggcttccc gatccgtggg cggcgccgga gaccccgtaa acacaacaca
      241 cgaacaagga agcgccgagg atggagagga cacagacgat cgcgggccgc ggcccgatca
      301 gccagatcgc ctggctgacc gaggatctcg atcgcgcgat cgaccagtgg cgccgcctcg
      361 cgggtgtcgg tccgtggacc gtctatcgca acgtcaccct cgacgacctc tatcgcggcg
      421 cccccgccat gatccgcatc gatgtcgggc tggcctggca gggcgagacc cagatcgagc
      481 tgatcgcgcc gcacggcacc ggtccctcac cctatcatga cgcgggcggg cgcgtgcgca
      541 cgggcatgca ccatgtcgcc tggctggtcg acgatgtcgc gacggccgcg accgatgcca
      601 cggccgcggg ccttgttccc gtcttcagcg ccgagagcgg cggcggtgcg acgcgtgtcg
      661 cctatttcga ggcgcccgac gaacccgcga tcctgctcga actgatcgag gcgacgccga
      721 cgatccgcga gggcttcgcg gccggaatcg ccgccgcgcg cgattgggac ggcggcaatc
      781 ccgtcaccat cttcgacttc gcagcctgaa ggagcttgca tggaaaagat catttacgcg
      841 ctgtggaaag accctgcgga ggacgatgag cgcttcaatg cacgcctgct cggcgacatc
      901 gccgcgcggc tcggcgaact ggcccatgcg gtgcgcgtca atgtgcagga tgatgatgtc
      961 gcgaccggca cctcgccgcg catgacctcg acgcgcccgc agatggacgc cgtgatccag
     1021 ctctggctgc cgagcgcgaa cgatcccgag cgcgccaccg tcgatgcgat catcgcggac
     1081 gccgcgccgc gtcatgccgc ctggctcgtc agcgaatcga cgatcatcgc caacacgcag
     1141 catccacccg cgcccggcgc gcggaccgag ggcttttcgc agatcgtgtt tctgggcctg
     1201 ccgccgcggc tgagccacga cggctggcgc gacgtctggc agcgccagca ttcggcggtg
     1261 gcggtcgata cgcaaagcaa tttcgaatat gtccagaatc tgatcatacg cccgctgagc
     1321 tatggcgccc ccgcctatgc cgcgatggtc gaggaatgtt tccccgccgc cgcgctgacc
     1381 gacgaggcgg tctatttcga tgcggcggga gaccccgcgc ggctcgccga aaatcagcgg
     1441 ctgatgatgg aaagctgcgc gcgcttcatc gatttcgacc ggatcgactg catcccgacg
     1501 agccagttcg atattcggcc gttcgcgcgc tgaacccccg aaaaccgaaa ggacagatca
     1561 tgcgtaatgt cgatgaatcg ctgacccggc tcgatcccga ctccatcgtc cgctcggacc
     1621 gtgtcgagat cgacgctccc gccgcgatcg tctgggagat actggtcgac cttccccgct
     1681 atggcgaatg gaatcccttt tgcatcgccg ccgaatcgac gctcgaaatg ggcgcgccgg
     1741 tgcatatgac gctccaaagc tataccgaac ccggtgcggt ctttcccaat tgcgaatatg
     1801 tctgcgcgtt cgagcccgaa cggctcctgt cgtgggaact gccgcacgac gacgcctggc
     1861 cctatcccgc gcggcgcgac cagatcatcg aggcgctcgg cgacgaattc tgcgcctatc
     1921 actcgaccga cgcgtttctc ggccccaacg gcattcacgt catgcgcttc tgcggcccgt
     1981 gggtgaagcg cgccttcgac gacaccgccg ctgcgctcaa gacgcgcgcc gaagcgctgc
     2041 accgcgaacg caagaccgct tcctgagcca tcgcccgcgc gtcgcacggg ctcgctctca
     2101 accaaaaaga aggccggata ccgcgctctt gtgcggtatc cggcttagtt gctcctgagt
     2161 gcccccgaga gatcggaagg agagttcagg ggagagatcg ggtcagaatt tgaaccccag
     2221 ctcgacgccg aagctgcgcg ggtcgccgta catcacgccg gcgaagccca gcgagccgaa
     2281 gtcgatcccc gagagcagat aatcctcctt ggtcagattc ttgccccaca ccgcgatcga
     2341 agcccgcgcg ccgcccaccg caatgtcgga aatcgacagc cgggcgttca gcaatcccac
     2401 gccgccatcg gagatttctt cgttatacgg gttgaccggg tgccaataga tcttgctgcg
     2461 atagttccag tcggcgcgcg caaccagctt gccgaaattg ccgagttcgg cctcatattg
     2521 cgcgcccaca ttggcggtcg tcgcggccga atattggaac ttcgcgacat cggaaatatc
     2581 ggtggccacg ccggtcaccg ggtcgacgaa ggtgaaattc ttatatttgc ggtcggtata
     2641 gccgaacgac gcgtcgacgg tgaagccatc ggcaatctgc gccagaatct cgccctcgat
     2701 ccccgtatag gtggccttcg cggcattgac ggtcgtgctc gacgccccgc cggttccggc
     2761 aaggaattgc tgcacctgca gatcgtcgta gatcgcgtgg aaagcggcaa ggttgagccg
     2821 caggcgccga tcgaacaact cgctcttgat tccgacctcg taggaaacga gatcctcggg
     2881 attgaaacca ttgttgtcgg ccgcgcgcgc gttgaagccg cccgccttat agcccgtgct
     2941 ggcacggaca taggtcatga tgtcatcgct ccagtcgtag gagatgttcg cgagccagtt
     3001 gagccggttg aacttgcgcg tcaggtcgcg cggcgcgggc ggcaccggcg ccgactggat
     3061 cagatgcttc ttgtcctcgg tatagcgcag cccgccggtg atgccgagcc ggctgtcgag
     3121 cgcggcgggt ttccaggtga gctggctgaa cgccgccatc gagcgcgaga cgtggcgata
     3181 ggacagcaaa gtttcgaggt tgacgccgaa gctgttcaga tcgacccccg gcgcgatcgg
     3241 aatcggcacc ggcgacggga ggacgagcgt gaagctctgc ggattgatct cggtcgcgcg
     3301 ctcgcggaaa taataggcgc cgagcacata gtcgaactcg tcgccgatcg acccgatcag
     3361 gttgaattcc tgtgaaaact gccgctggcg ccgttcgttg cgggtgctga acagatcgat
     3421 cgggccgacg ccctgcggga tgaaggcgtt gggcggcacc agaatcgcgg ggctgacgag
     3481 gaagccgacg aggccgccgt tgccgtcgag atccgagctg tcgctgcggt tgtcccattt
     3541 gcgatagccg gtgatcgagc gcagcgtcgc atcgccgatc tccagattga gcgtcagcga
     3601 atggccctgc accttgtcat gggcattgcc gtcgcgatcg agccggatct gcgacagccg
     3661 gtcctgcgaa acctgcggtg ccgtcccgcc gaacgcgggc gagaagttca gatattcgag
     3721 aatgtcgggg cggatcgcgc gcaactggaa cgcgttggcg cggccgttcc tgtcgctcga
     3781 atcgaagaca taatcgacgg taaagccggt gcccttgtcg aaacgcaccg cggcgcgaac
     3841 cgcgtcggtg ttgcgcgcgc ccggatcgcg cttgtccgac ggttcgagca gattgtcgac
     3901 cgtcccgttg cgctgactgt gataatagcc gagcttgagc gcgagcccgg tgtcgccaag
     3961 ttcgccggta tcgatcagcg ccttcaccat atattcgtcg aagctgccgt agccgacctg
     4021 caggcgcccg ccgaaatcct tcgacggctt ggccgagatc aggttgaccg cgccgccggt
     4081 cgtgttgcgg ccgtaaagcg tgccctgcgg gccgcgcagg acctcgacgc gctcgagatc
     4141 catcatgtcg aacagcgcgc ccgccgaccg gccgacgatg acgccatcga cataaaggcc
     4201 gaccgcgggg tcggcggtca gaatcgggtc gttgtcgacg ataccgcgga tcgagatcga
     4261 gggattggcc gtcgccgacg gcgtttcggt gatgatcagg ttgggggccg cgctgccgag
     4321 cgcggcgacg ctggtgatcc cgcgctgctc aatcatctcg gagttgatcg cgctgatcgc
     4381 gagcggcgtg tcctgaaggc tttcggagcg cttctgggcg gtgacgatga tctcgtcgag
     4441 acctccggtc gtggtctctt ccgcggcggg cgcggcctct tgcgcgtgcg cggtgctcgc
     4501 cacccagggc gcgatcagcg cgatggcggc gatttgcgcg cggcaatatg tgcgatgctt
     4561 catcagtccc atccctcttt ctgtctattg cggcgtatca tgcgcaattt acgggtcgaa
     4621 tatcacgata tgcgtaacat aagcaatata taactacgca agagggcaat gccctacccc
     4681 cttttggtgg tgggcgcggg gacgaaaaaa aggggcggca aagccgcccc aagtcaccca
     4741 aggagtctcc ccatggcaaa tcgttcaaat tcccggctgt tctttcggga tatggacccg
     4801 aaggccgtcc agttcctcgg tgatatggat ctggcagctc aatcggctgg tcggcttgac
     4861 ctcgatgaca tcggcgagcg catcgagcat ctcggcctcg tcgtcgcccg gcgggccgac
     4921 gcgcgccgcc caatcgtcgt cgacatagac gtgacaggtg gcgcactggc gcacgccgcc
     4981 gcacagcgcg tcgatcccgt cgaccagatt gccgatggca aggtgcatca ccgccatgcc
     5041 ctcgaaattg gtgcacgtct tttcgctgcc gtcgggctga acataggtga cgcgggccat
     5101 cagtcgtggc ccttcgcgtg cgccgggcat cccgccgctg ccgcgagcgc ctccggatcg
     5161 gcgggcgcac
//
LOCUS       DATMSU010000352         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_176611,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000352 DATMSU010000000
VERSION     DATMSU010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176611"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1179
                     /locus_tag="VN034_09515"
     CDS             55..1179
                     /locus_tag="VN034_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWV60881.1"
                     /translation="MPLFRLTSPGPFFDNKVRAFWNLQILGWAAWLGLRGVSGLANGQ
                     AFTFFIPQTISAITGFSLTLILSVCYRALINRRPLLMWGVSFGLAGVATALWAFIDAW
                     VAQIQNPASEAGFTSLLLGAMYIDATSLAAWSALYFAINYFLQLEEQNDRVLRLEAQA
                     ASAQLAMLRYQLNPHFLFNTLNSISTLVLLKQAEPANAMLSRLSAFLRYTLANEPTAQ
                     VTLAQEIETLKLYLDIEKMRFEERLRPHFAIDPAVSRARLPSLLLQPLIENAIKYAVT
                     PQEEGADITISAQLAGQNVRITVSDTGAGLSADPSDPTTGVATESTGVGLANIRDRLA
                     QAFGDQQRFDVHMGADGGFTVVIEFPFQPDGQMTIGTERT"
     gene            1176..1982
                     /locus_tag="VN034_09520"
     CDS             1176..1982
                     /locus_tag="VN034_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWV60882.1"
                     /translation="MTIRTILVDDEKLATQGLQLRLEAHGDVEIVDTAQNGREAIRKI
                     KTHKPDLVFLDIQMPGFDGFSVIQGLMEVEPPLVVFVTAYSDHAIRAFEAQAVDYLVK
                     PVEPERLADALDRVRQRLTEKRGAAEVERLKTVLAEVAPEAVEDYDAETQPDAHAADR
                     YEKMINIKDRGQIFRVDVDSIERIDAAGDYMCIYTADNSLILRETMKDLEKRLDPRNF
                     QRVHRSTIVNLSQVKQVKPHTNGECFLVLGSGAQVKVSRSYRDVVARFVH"
     gene            complement(2128..>2788)
                     /gene="ribA"
                     /locus_tag="VN034_09525"
     CDS             complement(2128..>2788)
                     /gene="ribA"
                     /locus_tag="VN034_09525"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWV60883.1"
                     /translation="FFVREAAGEAETFCTAGDVAALLDPTRLEVAARARLPVEASESA
                     EIVAFRSPEEASDHVALIIGQRDAGPPVVRLHSECLTGDVLGSLKCDCGPQLHAALHA
                     MADAPWGVLLYLRQEGRGIGLVNKLRAYALQDQGYDTVDANLRLGFPVEARDFAIAGR
                     MLDLLSIPRIRLMTNNPEKVARLEKEGVEVVERIPLALPTNKYNEQYLATKRDRTGHQ
                     L"
BASE COUNT          514 a          908 c          857 g          509 t
ORIGIN      
        1 atcgcgcgca cggccactga cacggtcatt gacatagcca acgcacggac cctcatgccg
       61 ctgtttcgcc tgacttcgcc cgggcccttc ttcgacaaca aggtgcgggc cttctggaat
      121 ttgcagatat tgggctgggc cgcgtggctc gggctgcgcg gggtgtccgg gcttgccaac
      181 gggcaggcct tcaccttttt cattccgcag acgatttcgg cgatcaccgg cttttcgctg
      241 acgctgatcc tgtcggtctg ctaccgcgcg ctgatcaacc gccgcccgct cttgatgtgg
      301 ggggtcagct tcggcctcgc cggcgtcgcg accgcgctct gggcctttat cgacgcgtgg
      361 gtcgcgcaga tccagaaccc cgcgagcgag gccggcttca ccagcctttt gctcggcgcg
      421 atgtatatcg acgcgacctc gctcgcggcc tggtcggcgc tctatttcgc gatcaactat
      481 ttcctccagc tcgaggaaca gaatgaccgc gtgctgcggc tcgaggcgca ggcggcctcg
      541 gcgcagcttg cgatgctgcg ctatcagctc aatccgcatt tcctgttcaa cacgctgaac
      601 agcatctcga cgctcgtcct gctgaagcaa gccgagcctg ccaatgcgat gctgtcgcgc
      661 ctttcggcct tcctccgcta cacgctcgcc aacgagccga ccgcgcaggt cacactggcg
      721 caggagatcg agacgctgaa gctctacctc gatatcgaga aaatgcgttt cgaggagcgg
      781 ctgcgtcccc atttcgcgat cgatcccgcg gtttcgcggg cgcgcttgcc gtcgcttttg
      841 ctccagcctt tgatcgaaaa cgcgatcaaa tatgcggtga cgccgcagga ggaaggcgcc
      901 gatatcacga tttccgcaca gttggccggt caaaatgtcc ggatcaccgt gtccgacacc
      961 ggcgcgggat tgtcagccga cccctcggac cccaccactg gcgttgcaac ggaatcgacc
     1021 ggtgtgggtt tagccaacat cagggaccgg ctggcgcagg cttttggcga tcagcaaagg
     1081 ttcgacgtcc atatgggcgc tgatggcgga ttcacggtgg tgatcgagtt tccgttccag
     1141 cccgacgggc aaatgacgat tggaaccgaa cgcacatgac gatcagaacc atcctggtgg
     1201 atgatgaaaa attggcgacc cagggcctgc aactcaggct cgaagcgcat ggtgatgtcg
     1261 aaatcgtcga caccgctcag aacggccgcg aggccatccg gaaaatcaag acccacaaac
     1321 ccgacctcgt tttcctcgac atccagatgc cggggttcga cggcttttcg gtgatccagg
     1381 ggctgatgga ggtcgaaccg ccgctcgtcg tcttcgtcac cgcctattcg gaccatgcga
     1441 tccgcgcctt cgaggcgcag gcggtcgact atctcgtgaa acccgtcgag cccgaacggc
     1501 tcgccgacgc gctcgaccgc gtccgccagc gcctcaccga aaagcgcggc gcggctgagg
     1561 tcgaacggct gaagaccgtg ctcgccgaag tagcgcccga agcggtcgag gattatgacg
     1621 ccgagacaca gcccgacgcg catgccgccg atcgttatga aaagatgatc aacatcaagg
     1681 atcgcggcca gatcttccgc gtcgatgtcg acagcatcga gcggatcgac gccgcaggcg
     1741 actatatgtg catctatacc gccgacaaca gcctgatcct gcgcgagacg atgaaggacc
     1801 tcgaaaagcg gctcgacccg cgcaacttcc agcgcgtcca ccgctcgacg atcgtgaacc
     1861 tcagccaggt caagcaggtc aagccgcaca ccaacggcga atgcttcctc gtgctcggat
     1921 cgggcgcgca ggtgaaggtc agccgcagct atcgcgacgt ggtggcaagg ttcgtgcatt
     1981 gatagatagc cctccccctt gatgggggag ggttgggtgg gggtgaggag gcaggcgtag
     2041 acttacgcag gcgacctcac ccccaccgct gcgactaggc catgaatggc caagtctcgc
     2101 tgcctccccc atcaaggggg agggatatca aagctggtgc cccgtccgat cgcgcttcgt
     2161 cgcgagatat tgctcgttat atttgttcgt cggcagcgcg agcgggatgc gctcgaccac
     2221 ctcgacgccc tctttctcca gccgcgcgac cttttcggga ttattggtca tcagccggat
     2281 gcgcgggatg ctcagcagat cgagcatccg ccccgcaatc gcgaaatcgc gcgcctcgac
     2341 cggaaaaccg agccgcagat tcgcatcgac cgtgtcatag ccctggtcct gcagcgcata
     2401 ggcgcgcagc ttgttgacga gcccgatccc gcgcccctcc tgcctgagat agagcagcac
     2461 gccccacggc gcgtcggcca tcgcgtggag cgcggcgtgg agctgcggcc cgcaatcgca
     2521 tttgaggctg ccgagcacgt cgccggtcag gcattcgctg tgcagacgaa caacgggcgg
     2581 acccgcgtcg cgctggccga tgatcagcgc gacatggtcc gatgcttcct cgggcgagcg
     2641 gaaggcgacg atttcggcgc tttcgctggc ttcgacgggc aggcgcgcgc gcgccgcgac
     2701 ttcgagccgc gtcgggtcga gcagcgcagc gacatcgccg gcggtacaga aggtttcggc
     2761 ttcccccgcc gcttcgcgca cgaagaaa
//
LOCUS       DATMSU010000353         6490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_15474, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000353 DATMSU010000000
VERSION     DATMSU010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15474"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..883
                     /gene="mtgA"
                     /locus_tag="VN034_09530"
     CDS             176..883
                     /gene="mtgA"
                     /locus_tag="VN034_09530"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HWV60884.1"
                     /translation="MATSSRAKKRKLPWYLRPFKWLLWFIVISVVWVLVYAVVPPPVT
                     LTMLLDSNGITKDWESLSNIDRNMVRAVIAAEDGKFCSHNGFDRDAIEEAIERNAKGK
                     KLRGGSTVSQQTAKNVFLWQGSGWTRYVRKVPEVWFTFLIETIWSKRRIMEVYLNVAE
                     TGIGTYGVEAGAQRYFHHGAAKLTPREAARIAAILPLPKKREAISPSGFTRRYGNTIT
                     ARIGVVRRDGLDACVYK"
     gene            complement(944..1480)
                     /locus_tag="VN034_09535"
     CDS             complement(944..1480)
                     /locus_tag="VN034_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWV60885.1"
                     /translation="MSRAKRSDDWGFPRWRPYGSSRETQKVRLCDRHGCTNPGNCPAP
                     KSPNSPERWYFCEAHAAEYNRGWDYFAGLSAEDAAARAAEEAQGARSYARAQHYTWGG
                     SGDGSRSADEMRALEILDLEPDADFEATKKAWRNLAKECHPDVKPGDAEAAKRFAAGQ
                     AAFEVLKQAEERKSWKPA"
     gene            complement(1536..2321)
                     /locus_tag="VN034_09540"
     CDS             complement(1536..2321)
                     /locus_tag="VN034_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HWV60886.1"
                     /translation="MSISLSDYETGAKYDGDYSEDLGALEDRLERLQAAHIIHGQRSI
                     IMFEGWDAAGKGGIIKRLTASLDPRFFEVWPIGAPNEEEKARHFLWRFWKRLPGNREI
                     SIFDRSWYGRVLVERVEGFASEAEWRKGYDEINEFEAQLTGSATHLVKLFIHITQEEQ
                     DARFAARLDDPWKRWKTGPEDYRNRSKRKDYLAAIEEMFAQTNTRWAPWKVIDGNHKK
                     TARIAALTHIVETLEAAVPMTPPDLDPAVVKLAAKAFGYKPED"
     gene            complement(2421..3293)
                     /locus_tag="VN034_09545"
     CDS             complement(2421..3293)
                     /locus_tag="VN034_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV60887.1"
                     /translation="MSEPREHRLETPEGEICWFEWGTPGQGASLLLLHATGFHARLWD
                     QVVAALPSGTHVIAPDHRGHGRSYRPATLASWTATADALLPLLDGLGGHPLVGCGHSM
                     GAYVLTRLASQRPAAFAHLVLIDPVIMDPAFYEGESAKPIPDPAGHPVARRRNAWASA
                     EDMRARFADRPPYANWDPRVLEDYCTHGLLPAADGEGLELACPPALEASVYQNALRTS
                     PHEWLHYLSVPSTLIRAPTGERGGELDFSLSPTWTGLGPAIGAERDELWAEHSHFIPM
                     ENPARVAALLGNFL"
     gene            complement(3297..5078)
                     /gene="aspS"
                     /locus_tag="VN034_09550"
     CDS             complement(3297..5078)
                     /gene="aspS"
                     /locus_tag="VN034_09550"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWV60888.1"
                     /translation="MHAYRTHNCGQLRAEDVGQNVRLSGWVHRKRDHGGLLFVDLRDH
                     YGLTQIVADSSDAAFATLDGLRVESVVTITGDVVARSAETTNANLPTGAIEVRAREVA
                     VQSAAIELPMPVAGEQEYPEDIRLKYRFLDLRRDRLHQNILLRSNVIASLRRRMVEQG
                     FTEYQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQMFKQLLMVAGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDFEMSFVTQDDVFNAIEPVLAGVFEEYANGKSVTPAGSF
                     PRIPYRESMLKYGNDKPDLRNPLIITDVSSHFAGSGFGRFADIVAAGDVVRAIPAPAT
                     AEKSRKFFDDMNSWAQGEGFAGLGYATRKGGEWGGPIAKNHGSDKMDALAAELGIGPD
                     DGLFFAAGKEAIAAKLAGFARTRVAEQLELIDANKFEMCWIVDFPMFEADEDTGKIDF
                     SHNPFSMPQGELEALETKDPLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFEIAG
                     YSQADVDANFSGMINAFKFGAPPHGGSAPGVDRIVMLLADEPNIREVVVFPMNQKAED
                     LMMGAPAPVSEKQLKELSIRLVDGPKN"
     gene            complement(5179..>6490)
                     /locus_tag="VN034_09555"
     CDS             complement(5179..>6490)
                     /locus_tag="VN034_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWV60889.1"
                     /translation="GWSPSNVNIASQSWLLHTRRFNRCFRIGTDDVRPGVDAAALMLV
                     EAGALIDEVSDKLEAQGRSLWTSGAGNGQTFAGACATGTHGSMIARGGIQDHIRAVQV
                     VTPGGIHWIEPETGVMSDAFIAATGSTPLRDDDVFAAAQLPVGALGIITALVVDSVPR
                     FLVRPIQNLRKVAPGALDWLAAGDFRRFSAAYALDVDPVFVQMIVNPYKPFKRPAMLR
                     FLYREPWRDDYPRATPGQLGAGYDALSLLGRLLDDYPWARGPLLQFAMKMGYAAGPDV
                     DDPPVYGSWGEGLDTHRPMADLFNASVTIDRADLAHAFERICAVYARHGGSTVVTIRF
                     MERAQGLLAPARFTHNAVIDFDGPRSRRTADAYARVVECLDAEGIAFTRHWAKSCRLD
                     AARVAADYGEDFRRWRAAQGRLMPDPAHRALFGSEVLDGLGLTC"
BASE COUNT         1091 a         2077 c         2209 g         1113 t
ORIGIN      
        1 gcaaagggcg cggcggtcct cttgcccgag cgccggcgag gcgaaggcga gcgcggcgag
       61 gagcaggacg gatcgaagcg gcatcggggc cttcctttca tcgcttttct ttccgaaatt
      121 cgatcaaccg atcaagcccg cttgcctcga aacggcaagc ggttaatttg ccgccatggc
      181 aacctcctcc cgcgcgaaga agcgcaagct cccctggtat ctccgtccgt tcaaatggct
      241 gctctggttc atcgtcatct cggtggtgtg ggtgctcgtc tacgcggtcg tgccgccgcc
      301 ggtgacgctg acgatgctgc tcgacagcaa cggcatcacc aaggattggg aaagcctgtc
      361 gaacatcgac cgcaacatgg tgcgcgcggt gatcgcggcc gaggacggca aattctgcag
      421 ccataacggc ttcgaccgcg acgcgatcga agaggcgatc gagcgcaacg ccaagggcaa
      481 gaagctgcgc ggcggatcga cggtgagcca gcagaccgcg aagaatgtat tcctgtggca
      541 gggcagcggc tggacgcgct atgtccgcaa ggtgcccgag gtatggttca ccttcctgat
      601 cgagacgatc tggagcaaga ggcggatcat ggaggtttac ctcaacgtcg ccgagacggg
      661 catcggcacc tatggcgtcg aggcgggcgc ccagcgctat ttccatcatg gcgcggccaa
      721 gctgaccccg cgcgaggcgg cgcggatcgc cgcgatcctg ccgctgccga agaagcgcga
      781 ggcgatcagc ccgtcgggct tcacgcggcg ttatggcaac acgatcaccg cgcgcatcgg
      841 tgtagtgcgg cgtgacgggc tggatgcgtg cgtttataaa tagcggtggc ttggggatgg
      901 cggttaacag ccggcaccag ccaagccgct gaccggatcg ccgctaggcc ggtttccagc
      961 tcttacgctc ttcggcctgc ttcagaacct cgaacgccgc ttgcccggcc gcgaagcgct
     1021 tcgcggcctc ggcgtcgccg ggcttgacgt cggggtgaca ttctttcgcc agattgcgcc
     1081 acgccttttt cgtcgcctcg aaatcggcat cgggttcaag gtcgaggatt tcgagcgcgc
     1141 gcatttcgtc ggcgctgcgg ctgccgtcgc ccgagccgcc ccatgtgtaa tgctgtgcgc
     1201 gggcatagct gcgcgcgccc tgtgcctcct cggcggcgcg cgcggcggcg tcctcggccg
     1261 aaaggccggc gaaatagtcc cagccgcgat tatattcggc agcgtgcgct tcgcagaaat
     1321 accagcgttc ggggctgttc ggcgatttgg gcgcggggca attgccgggg ttggtgcagc
     1381 catggcggtc gcacaggcgc accttctgcg tctcgcgcga cgagccatag gggcgccagc
     1441 gggggaaacc ccagtcgtca gatctcttgg cgcggctcat ttcagtccat gtagcggctg
     1501 agaccccgga ttgctagccg agcggggcga aaaacctagt cttcgggctt gtagccgaac
     1561 gctttcgccg cgagcttgac gacggcggga tcgagatcgg gcggcgtcat cggcaccgcg
     1621 gcctcgagcg tttcgacgat atgggtcagc gccgcgatac gcgccgtctt cttgtggttg
     1681 ccgtcgatca ccttccacgg cgcccagcgc gtattcgtct gcgcgaacat ctcttcgatc
     1741 gcggcgagat agtccttgcg cttcgaccgg ttgcgataat cttcggggcc ggtcttccag
     1801 cgcttccacg gatcgtcgag gcgcgcggcg aaacgcgcgt cctgctcctc ctgcgtgatg
     1861 tggatgaaca gcttgacgag gtgggtagcg ctgccggtca actgcgcctc gaactcgttg
     1921 atctcgtcat agcccttgcg ccattcggct tcgctcgcga agccctcgac gcgctcgacg
     1981 aggacgcggc cgtaccagct gcggtcgaag atcgagattt cgcgattgcc cggcaggcgt
     2041 ttccagaagc gccagaggaa atggcgcgcc ttttcctctt cgttcggtgc gccgatcggc
     2101 cagacctcga aaaagcgcgg gtcgagcgac gcggtcagcc gcttgatgat gccgcccttc
     2161 cccgccgcgt cccagccttc gaacatgatg atgctgcgct ggccgtggat gatatgcgcg
     2221 gcctggagcc tttcgagccg atcctcaagc gccccgagat cctcggaata gtcgccgtca
     2281 tatttggcgc cggtttcata atcggaaagg gagatgctca tccgctcacc ctagcgcgcg
     2341 tcgccctgcg caccaagcgg gcgatgcgcc gatgtcgttt tctggcacag cgcgcgccga
     2401 tttcattgcg cgtgtccgta tcagagaaaa ttgccgagca gcgcggcaac gcgcgccgga
     2461 ttctccatcg gaatgaaatg gctatgctcg gcccatagct cgtcgcgctc ggcgccgatc
     2521 gccggaccga ggccggtcca ggtcgggctc agcgaaaagt cgagctcgcc gccgcgttcg
     2581 ccggtcgggg cgcggatcag cgtcgagggc accgacagat agtgcaacca ctcgtgcgga
     2641 ctggtgcgca gcgcattctg atagaccgat gcttcgagcg ccggcggaca ggccagctcg
     2701 aggccctccc cgtcggcggc gggaagcagg ccatgggtgc agtaatcctc gagcacgcgc
     2761 ggatcccagt tcgcataagg cggccggtcg gcgaagcgcg cacgcatatc ttccgcgctg
     2821 gcccatgcgt tgcgccgccg cgccaccgga tggccggcgg gatcgggaat cggcttggcg
     2881 ctctcgccct cgtaaaaggc ggggtccatg atcaccggat cgatcaagac gaggtgagcg
     2941 aaggccgccg gccgctgcga cgcgagccgg gtgagcacat aggcacccat gctgtgaccg
     3001 cagccgacga gcggatggcc gccgagcccg tcgagcagcg gcagcaacgc atcggcggtc
     3061 gccgtccagc tggcgagcgt cgcgggacga tagctgcgcc cgtgaccgcg atggtcgggc
     3121 gcgatgacgt gcgtgcccga aggcaaggcc gcaacaacct gatcccacag ccgcgcgtga
     3181 aagcccgtcg cgtggagcaa gagcagcgat gccccctgcc ccggcgtgcc ccattcgaac
     3241 cagcaaatct cgccttccgg cgtttccagc cggtgttccc ggggttcgct catcccttag
     3301 ttcttcggac cgtcgacgag gcggatgctg agttccttga gctgcttttc gctgaccggc
     3361 gcgggggcgc ccatcatcaa atcctcggcc ttctggttca tcgggaagac gacgacttcg
     3421 cggatgttcg gctcgtcggc gagcagcatc acgatgcggt cgacacccgg cgccgaaccg
     3481 ccatgcggcg gcgcgccgaa cttgaaggca ttgatcatgc cgctgaagtt cgcgtcgacg
     3541 tcggcctgcg aatagcccgc aatctcgaac gccttataca tgatgtcggg gcggtggtta
     3601 cggatcgcgc ccgacgagag ttcgacgccg ttgcagacga tgtcatattg ccacgcgagg
     3661 atgtcgagcg ggtccttggt ttcgagcgcc tcgagctcgc cctgcggcat gctaaacggg
     3721 ttgtggctga agtcgatctt gccggtgtcc tcgtcggcct cgaacatcgg gaagtcgacg
     3781 atccagcaca tttcgaactt gttcgcatcg atcagctcaa gctgttcggc gacacgggtg
     3841 cgcgcgaaac cggcgagctt ggcggcgatc gcttccttgc ccgcggcgaa gaagagcccg
     3901 tcgtcgggac cgatgccgag ctcggcggcg agcgcgtcca tcttgtcgga cccatggttc
     3961 ttggcgatcg gaccgcccca ttcgccgccc ttgcgcgtcg cgtagccgag gcccgcaaag
     4021 ccctcgccct gcgcccagct gttcatgtcg tcgaagaatt tgcggctctt ctcggcggtc
     4081 gcgggtgccg gaatcgcgcg cacgacgtcg cccgccgcga cgatgtcggc gaagcggccg
     4141 aagcccgatc cggcgaaatg cgacgagacg tcggtgatga tcagcgggtt gcgcagatcc
     4201 ggcttgtcat tgccgtactt cagcatcgat tcgcggtacg ggatgcgcgg gaacgaaccg
     4261 gcgggggtca ccgacttgcc gttcgcatat tcctcgaaca cgccggcgag cacgggctcg
     4321 atcgcgttga agacgtcgtc ctgcgtgacg aagctcatct cgaagtcgag ctgatagaat
     4381 tcgcccgggc tgcggtcggc gcgcgcatcc tcgtcgcgga agcagggcgc gatctggaaa
     4441 tagcggtcga agccagcgac catcagcagc tgcttgaaca tctgcggcgc ctggggcagc
     4501 gcatagaatt tgccggggtg gacgcggctc ggcaccagat agtcgcgcgc gccctcgggc
     4561 gacgaggcgg tgaggatcgg cgtctgatat tcggtgaagc cttgttcgac catgcgacgg
     4621 cgcagcgacg cgatgacgtt cgagcgcagc aggatgttct gatgcaggcg gtcgcggcgc
     4681 aggtcgagga aacggtattt gaggcggatg tcctcgggat attcctgttc gcccgcgacc
     4741 ggcatcggca gctcgatcgc cgccgactgc accgcgacct cacgcgcgcg gacttcgatc
     4801 gcgccggtgg gcaggttggc gttggtcgtt tcggccgagc gcgcgacgac gtcgccggtg
     4861 atcgtcacga cgctttcgac gcgcaggccg tcgagcgtgg cgaaagccgc gtcgctcgag
     4921 tccgcgacaa tctgcgtcag cccgtaatga tcgcgcaggt cgacgaagag cagcccgcca
     4981 tgatcgcgct tgcgatgcac ccagcccgac aggcggacat tctggccgac gtcctcggcg
     5041 cggagctggc cacaattatg ggtgcgatag gcgtgcattg tctggtcttt caaatgtcgg
     5101 gagggcgcgc gcaaacgtgc cgatatggcc gcgcctaagg cagcgcggcg agcgatttgt
     5161 caagggcgga gggcgcaatc aacaggtcag cccgagcccg tcgagcactt cgctgccgaa
     5221 cagcgcccgg tgcgcgggat cgggcatcaa gcggccctgc gccgcgcgcc agcggcgaaa
     5281 atcctctcca tagtcggcgg cgacgcgcgc cgcgtcgaga cggcagcttt tcgcccagtg
     5341 ccgggtgaag gcgatcccct cggcatcgag gcattcgacg acgcgcgcat aagcgtcggc
     5401 ggtgcgccgg ctgcgcgggc cgtcgaaatc gatcaccgca ttgtgcgtga aacgcgccgg
     5461 cgcgagcaga ccttgcgcgc gctccatgaa acgtatggtg acgacggtcg agccgccatg
     5521 gcgtgcataa accgcacaga tccgctcgaa cgcgtgcgca aggtcggcgc gatcgatggt
     5581 gaccgaggcg ttgaacaggt ccgccatggg gcgatgcgtg tcgagcccct cgccccagct
     5641 gccatagacg ggcggatcgt cgacgtcggg gccggcggca tagcccatct tcatcgcgaa
     5701 ttgcagcagc ggcccccgcg cccatgggta atcgtcgagc agccgtccga gcaggctgag
     5761 cgcgtcatag cccgcgccga gctggccggg cgtcgcgcgg ggataatcgt cgcgccacgg
     5821 ttcgcgatag aggaagcgca gcatcgcggg ccgcttgaac ggcttgtagg gattgacgat
     5881 catctggacg aaaacagggt ccacgtcgag cgcataggcg gccgagaagc gccggaaatc
     5941 gcccgcggcg agccagtcga gcgcgccagg ggcgacctta cgcagattct ggatcgggcg
     6001 gacgaggaat cggggcacgc tgtcgacgac gagcgcggtg atgataccga gcgcgccgac
     6061 cggaagctgc gccgcggcga agacatcgtc gtcgcgcagc ggtgtcgatc cggtcgcggc
     6121 gatgaacgcg tcgctcatca cgccggtctc cggttcgatc cagtggatgc cgccgggcgt
     6181 gacgacctgc accgcgcgta tatgatcctg aataccgccg cgcgcgatca tcgagccgtg
     6241 ggtgccggtg gcgcacgcgc ccgcgaaggt ctggccgttg ccggcgccgc tggtccacag
     6301 cgagcggccc tgcgcctcga gcttgtcgga cacttcgtcg atgagcgccc ccgcctcgac
     6361 gagcatcagc gcggcggcat cgacgcccgg ccgcacatcg tcggtgccga tccggaagca
     6421 gcggttaaag cggcgcgtgt gaagcagcca gctttgcgag gcgatgttga cgttcgacgg
     6481 cgaccagccc
//
LOCUS       DATMSU010000354         4957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_100885,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000354 DATMSU010000000
VERSION     DATMSU010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100885"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..2841
                     /locus_tag="VN034_09560"
     CDS             10..2841
                     /locus_tag="VN034_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60890.1"
                     /translation="MKSSSLRAARFMRTSALGVSIAMAAFSVPAFAQDAPADTATDDS
                     DNTPIIVTAQGRSQLLSDVPVAISAVSAETLQNSGANDIRQLNQVAPSLLVSSTGSEA
                     NGSARIRGIGTVGDNPGLESSVPVFIDGVYRSRSGIGLNELGEIDRIEVQRGPQGTLG
                     GRNSSAGLISIYSKKPDFQFGATGEITYGNYDYWRLGGSVTGPISDTLAARLDGVWVK
                     RDGFLNDVNNDRDINNRDRYFLRGQLLFEPTDALSVRLIADYTSRDEECCGATYVGPS
                     VNQYIGDLNTPSPTGAATPTSNNIIRVLADLGQPLSAFNQGYSRDLSVTPGRSFAGKT
                     KDYGFSGQIDYDFGGATLTSITAYREYRSSQAGDLDYGTVDILYRAPDADAYRQFHTF
                     TQELRLQGEAFDGKLDWLVGGFYANEKLRVRDNLRFGEDYGRFATCRLITATPLAGLY
                     SPGSPACVLPFVAPGVPGLAAIAGANGASGPDIVAALTALDGLSDLGSINDVYRQDGE
                     NWALFTHNIFHITDKLDFTFGLRYTNDKKKFSASFTNDNTVCTTVQGLVLDDAAQPEI
                     PGTADNLARAVAGGLIGLACQGNSTAELDGVSINDKRSEDEWTGTAILSYKPVDDLMV
                     YASYSRGYKAGGFNLDRSALKSPILPFALSGGAQALVGNLQFDPETVDSYEIGAKYST
                     GPFGLGLTLFRSDFSSFQLNTFNGTVFLVQNVNGCDSDLGGGDRDQSKFTGAPNYNPA
                     AATTGACPADDVSHGVRSQGFELEASLVPARDFRMTAGLTYAKTKYRGQLVGNDSGAP
                     LDQALRLLPGNNLSNAPELVATGSVAWTPDIGSSGLTGLVYVDGRMTSDYNTGSDLFP
                     QKAQDGYALFNARIGIRGPDEKWGIEFWGQNIFNKQYAQVAFNSPFQEGATSTGAAFA
                     DPQYPGGRQIFSQFLAEPRTYGVTLRGKF"
     gene            3082..>4957
                     /locus_tag="VN034_09565"
     CDS             3082..>4957
                     /locus_tag="VN034_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60891.1"
                     /translation="MKNNCRRAAGLLRTSAAGLSLSLALVAVPAIAQDAAGAETDDST
                     IIVTATRRSEALSDVPIAVSAVTGDTLEKTGATDVRALGQVAPSLLVSGATSEVNFTA
                     RIRGIGTVGENPGLESSVGLFIDGVYRSRTGVGLSELGDIERVEVLRGPQGTLFGRNS
                     TAGLINIVTKGPDLSAFGAKGSVSYGNYDYWRIDGMINAPLGDKAAVRVDGVWQRRDG
                     FIDNATPGEPDINDRDRWLIKGQLLLEPAENITLRLIADYSKRNENCCGGVLLNPVRS
                     LTRGPDGAPVASPNTLLPLLQFLGANHQVPPAGDSFVRRQSTTPGVTYRSDTKDWGVS
                     GELDWDFGGATLTSITAYRDYKNAQGQDADFNTLDILRRTDLDRRFRLFTQELRLQGE
                     AFDGRLDWLVGGYYANEKLDVDDDIVYGADYQRYANCLAAAALAPTFLNPASPTCSNL
                     PAASFPGFQGIAALLGAAPLNGTGNNGSTFAQRSTNYAVFTHNSFDIVEDALTLTVGA
                     RYTHEKKTLSGDANFTNTLCPAIVNSSLQALATLACVINGTAPDIVAGAPGTKFSEGQ
                     WTGTAVLSWKPAPEWLIYASASKGYKAGGFNLDYSALDRPCSTTAGSDAQDAACVAAL
                     A"
BASE COUNT          882 a         1704 c         1534 g          837 t
ORIGIN      
        1 gtgcccgata tgaaatcatc ttcccttcgt gctgcccgat ttatgcgtac cagcgcgctt
       61 ggcgtctcga tcgccatggc agcgttctcg gttcccgcct ttgcccaaga cgcaccggcc
      121 gacaccgcaa ccgacgacag cgacaacacc ccgatcatcg tcaccgcgca gggccgctcc
      181 cagcttttgt ccgacgtccc cgtcgcgatc tcggcggtca gcgccgaaac gctgcagaac
      241 agcggcgcga acgacattcg ccagctgaac caggtcgcgc cgtcgctgct cgtttcgtcg
      301 accggctcgg aagcgaacgg ttcggcgcgt atccgcggta tcggcaccgt tggcgacaat
      361 cccggcctcg aaagctcggt cccggtcttc atcgacggcg tctatcgctc gcgttcgggc
      421 atcggcctca acgaactggg cgaaatcgac cgtatcgaag tgcagcgcgg cccgcagggc
      481 acgctcggcg gccgcaactc gtcggcgggc ctgatcagca tctattcgaa aaagcccgat
      541 ttccagttcg gcgcgacggg tgagatcacc tatggcaatt atgattactg gcgcctcggc
      601 ggcagcgtca ccggcccgat cagcgacacg ctcgcggcgc gcctcgacgg cgtgtgggtg
      661 aagcgcgacg gcttcctgaa cgatgtgaac aacgaccgcg acatcaacaa ccgcgatcgc
      721 tatttcctgc gcggccagct gcttttcgaa ccgaccgacg cgctgtcggt ccgcctgatc
      781 gccgattata cgtcgcgcga cgaggaatgc tgcggcgcca cctatgtcgg tccgtcggtc
      841 aaccaatata tcggcgatct caacacgccg agcccgacgg gcgccgcaac gccgacgtcg
      901 aacaacatca tccgcgtgct cgccgacctc ggccagccgc tttcggcgtt caaccagggt
      961 tacagccgcg acctttcggt caccccgggg cgcagcttcg ccggcaagac gaaggattac
     1021 ggtttctcgg gccagatcga ctatgatttc ggcggcgcga cgctgacctc gatcaccgcg
     1081 taccgcgaat atcggtcgag ccaggcgggc gacctcgact atggcacggt cgacatcctc
     1141 tatcgcgccc ccgacgccga cgcctatcgc cagttccaca ccttcacgca ggaactgcgc
     1201 ttgcagggtg aggcgttcga cggcaagctc gactggctcg tcggcggctt ctacgccaat
     1261 gaaaagctgc gcgtgcgcga caatctgcgc ttcggcgagg attacggccg tttcgcgacg
     1321 tgccgcctga tcacggcgac cccgctcgcg ggtctttatt cgcccggcag cccggcatgc
     1381 gttctgccct ttgtcgctcc cggcgttccc ggcctcgccg ccatcgcggg ggcgaacggc
     1441 gcatcgggcc cggacatcgt tgccgcgctg accgcgctcg acgggctgag cgaccttggc
     1501 agcatcaacg acgtctatcg tcaggatggc gagaattggg cgttgttcac gcacaatatc
     1561 ttccacatca ccgacaagct cgatttcacc ttcgggctcc gttacacgaa cgacaagaag
     1621 aaattctcgg cgagcttcac caacgacaac acggtctgca caaccgtcca gggactggtg
     1681 ctcgacgatg cggcccagcc ggaaattccc ggcaccgccg acaatctggc gcgcgccgtc
     1741 gcgggcgggc tgatcggcct ggcctgccag ggcaattcga ccgccgaact cgacggcgtg
     1801 tcgatcaacg acaagcgcag cgaggacgaa tggaccggca ccgcgatcct gtcgtacaaa
     1861 ccggtcgacg acctgatggt ctatgcaagc tattcgcgcg gttacaaggc gggcggcttc
     1921 aacctcgacc ggtcggcgct gaaatcgccg atcctgccct tcgcgctgtc gggcggggcg
     1981 caggcgctgg tcggcaatct gcagttcgac cccgaaacgg tcgacagcta tgaaatcggc
     2041 gccaaatatt cgaccggtcc gttcggcctc ggcctgaccc tcttccggtc ggatttctcg
     2101 agcttccagc tcaacacgtt caatggcacg gttttcctcg tccagaacgt caacggctgc
     2161 gacagcgatc tgggcggtgg cgaccgcgat cagagcaagt tcaccggcgc gccgaactac
     2221 aaccccgcgg cggcgaccac cggcgcctgt cccgccgacg acgtcagcca cggcgtccgg
     2281 tcgcaaggct tcgaactcga agcctcgctc gttccggcgc gcgacttccg catgaccgcg
     2341 gggctgacct atgcgaagac caaatatcgc ggccagctgg tcggcaatga ttcgggcgca
     2401 ccgctcgatc aggcgctgcg cctgctgccg ggcaacaatc tgtcgaacgc gcccgaactg
     2461 gtcgcgaccg gcagcgtcgc ctggaccccc gatatcggca gcagcgggct gaccggtctc
     2521 gtctatgtcg atggccgcat gaccagcgac tataacaccg ggtcggacct gttcccgcaa
     2581 aaggcgcagg acggctatgc gctgttcaac gcgcgtatcg gtattcgcgg ccccgacgag
     2641 aaatggggca tcgaattctg ggggcagaat atcttcaaca aacaatatgc ccaggtcgcg
     2701 ttcaactcgc cgttccagga gggcgcgacc tcgaccggcg ccgctttcgc cgacccgcaa
     2761 tatccgggcg gccgccagat tttctcgcag ttcctcgccg aaccgcggac ctatggcgtg
     2821 acgctgcgcg gcaaattctg atcgcaacga tcggatgaat tcgagggggc ggggccggtg
     2881 gcttcgcccc cttttctttg tccgaaccag tcaacccgtc acatctgaca gctgtgacac
     2941 aaaagccgcg cgcccggcag aatgacgtta gggcagcggc gccgcgctgg catgtcgcgc
     3001 gcaagtcgtg atacgcctcg gccataaaca aaaaagctct cccccgcacc gatgaacgat
     3061 cgtgagagga gggccatttt catgaaaaac aattgccgcc gcgccgcggg tctgctgcga
     3121 accagcgccg ctggactgtc gctttcgctc gcccttgtcg ccgtgcccgc catcgcgcag
     3181 gacgccgcgg gcgccgaaac cgacgacagc acgatcatcg tcaccgcgac gcggcgcagc
     3241 gaggcgctgt ccgacgtgcc gatcgcggtc tcggcggtca ccggcgacac gctcgaaaag
     3301 acgggcgcga ccgacgtgcg cgcgctgggg caggtcgcac cgtcgctgct cgtgtcgggc
     3361 gcgacgagcg aggtcaattt caccgcacgc atccgcggca tcggcacggt cggcgagaat
     3421 cccggtcttg aaagctcggt cggcctgttc atcgacggcg tctatcgcag ccggacgggg
     3481 gtgggcctgt ccgagctcgg cgatatcgag cgcgtcgagg tgctgcgcgg accgcagggc
     3541 acgctgttcg gccgcaactc gaccgcgggg ctgatcaaca tcgtcaccaa ggggccggac
     3601 ctttcggcgt tcggcgccaa gggatcggtt tcctatggca attatgatta ttggcgcatc
     3661 gacggcatga tcaacgcgcc gctgggcgac aaggcggcgg tgcgcgtcga tggcgtctgg
     3721 cagcggcgcg acggcttcat cgacaatgcg acgccgggcg agcccgacat caacgaccgc
     3781 gaccgctggc tgattaaggg ccagctgctc ctcgaacccg ccgagaatat cacgctgcgg
     3841 ctgatcgccg actattcgaa gcgcaacgag aattgctgcg gcggcgtgct cctcaacccc
     3901 gtgcgcagcc tgacgcgcgg gcccgacggc gcccccgtgg cgtcgcccaa cacgctgctg
     3961 ccgctgctcc agtttctcgg cgcgaaccat caggttccgc cggccggcga cagcttcgtc
     4021 cgtcgccagt cgacgacgcc cggcgtcact taccggtcgg atacgaagga ctggggcgtg
     4081 tcgggcgagc tcgactggga tttcggcggc gcgacgctga cctcgatcac cgcctatcgc
     4141 gattacaaga atgcacaggg gcaggacgcc gatttcaaca cgctcgacat cttgcggcgc
     4201 accgacctcg atcgccgctt ccggctgttc acgcaggagc tgcggctgca gggcgaggcg
     4261 ttcgatggcc ggctcgactg gctcgtcggc ggctattatg cgaacgagaa gctcgatgtc
     4321 gacgacgaca tcgtctatgg cgccgactat cagcgctatg ccaattgcct tgcggcggcg
     4381 gcgctcgccc cgaccttcct caacccggcg tcgccgacct gttcgaacct gcccgcggcg
     4441 agctttccgg gctttcaggg catcgccgcg ctgctcggcg ccgcgccgct caacggcacc
     4501 ggcaacaatg gatcgacctt cgcccagcgc agcaccaatt atgcggtctt cacgcataac
     4561 agcttcgaca tcgtcgagga tgcgctgacg ctgaccgtcg gcgcgcgcta cacgcatgag
     4621 aagaagacgc tgagcggcga cgccaatttc accaacacgc tctgcccggc gatcgtcaat
     4681 tcatccttgc aagccttggc gaccctcgcc tgcgtgatca acggcaccgc gcccgacatc
     4741 gtcgccggcg cgccggggac caaattcagc gaaggccagt ggacgggcac cgcggtgttg
     4801 agctggaaac cggcgcccga atggctgatc tatgcctcgg catcgaaggg ctacaaggcg
     4861 ggcggcttca acctcgacta ttcggcgctc gaccggccgt gcagcacgac cgcggggtcc
     4921 gacgcgcagg atgccgcgtg cgtcgccgcg ctcgcgc
//
LOCUS       DATMSU010000355         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_186195,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000355 DATMSU010000000
VERSION     DATMSU010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186195"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..2183)
                     /locus_tag="VN034_09570"
     CDS             complement(126..2183)
                     /locus_tag="VN034_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60892.1"
                     /translation="MKFRTAILSSCAAALTLTSHAARAEEGDTDAAGDIVVVGALTDV
                     AIDREEIEVLQANDLADLFRNVPSVSVGGGVGLAQKIYVRGLEDSLLNVTIDGAPQRG
                     TLFHHIGRVTIEPELLETVDVQAGPGEATAGFGAVGGAIRFRTRNAVDMLQDGRDIGG
                     IAKAGWFSNDGYKLSGTLYGRIAGDIGILASYVHVNRDPYKDGDGVRQLATGAEQNLG
                     FVKIGGELGGGHRITASYEQRRESGRFGQRPNWPVLAGDRLFEAKGKRQTAVLNYGFE
                     GDSGVGIEATGYWTRTNFTQNRDDRWGLYGAEIETYGFDARANLAIGNHDITVGAEHR
                     RDRVTSEYLDDPAMWQDWAWDPNVGRFTEEGALMGVYIQDHWRVVDPLLISFGARYDA
                     YDLDQTTYRTGTKSDGLSFNVGAEFEIVPGLSLNAGFAEAFRGKEIGDAFTLEKNPAW
                     LSMSPALRPERVDNLEGGVKYDLGGFTASAVYYEMTIKNVILDQLGRDRDARPIQDAV
                     YYENVGKFKSKGIELRAGYRAGPFSIDGYYNHYDSRLNDNRIEGYEHIALGNSMGDNW
                     AVTAGYQPSDYFNFRASLTRFEDLNNIEVLFRESQLGFIDGTRFIDKPGYTTVDLFGS
                     WRPFGSDRLELQAAVYNLFDKRYIAHASVADYGQLGLEDYAIVSGLPEPGRNVRLTAS
                     IRF"
     gene            2314..3000
                     /locus_tag="VN034_09575"
     CDS             2314..3000
                     /locus_tag="VN034_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60893.1"
                     /translation="MTETPNREAAPDAGAPANVAANLGSRFLRYLPLITIAHFVIGLP
                     ALIASLALAYFAFVQADATQKMQTGGVMPFVTFGTSNGDEDGNQDISLSLTNNGVGPA
                     ILGPIEIRYEGKPINTPVELLKTCCAKTGTQGLRLSTSPSTGIAVRPGETIEFVSFPR
                     TPESEKVWQTFNRERWKLKVRACYCSIFNDCWITEGMQGLPKPVNKCPADWSLYREDA
                     GNRIAGIEAK"
     gene            3050..3445
                     /locus_tag="VN034_09580"
     CDS             3050..3445
                     /locus_tag="VN034_09580"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWV60894.1"
                     /translation="MVEHDSHTLDTIFHALGDTTRRAMLGELAAGERTVGELAEPFAM
                     SLAAASKHIKVLEAAGLVRRDIRGRTHVCSLDPVPLMRADQWIGIYRRFWTDRLDVLE
                     KILRADDAAPPPPRNPSPGKKEKHDDDNA"
     gene            3429..3962
                     /locus_tag="VN034_09585"
     CDS             3429..3962
                     /locus_tag="VN034_09585"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV60895.1"
                     /translation="MTTMLDEGRYGLLTEPATLTITRLLPGPIDRIWAYLTDGDMRRQ
                     WLAAGAMEERAGAPIELVWRNDELTDPPGTRPEGFGDEHRMACEVVAVDAPRLLSISW
                     GSTGGVTFTLEEKGDEVLLTIVHRRVEDPAVLLNVSSGWHAHLDVLEARLRGTQAEPY
                     WDNWTRLRSEYAERLFG"
     gene            4052..4528
                     /locus_tag="VN034_09590"
     CDS             4052..4528
                     /locus_tag="VN034_09590"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWV60896.1"
                     /translation="MDDSETYLREAIALAYANAEKGGRPFGAVIVRDGRVIASAVNEI
                     LATGDPTSHAELNAIRLASKVVGSPDLAGCSVFASGHPCPMCMAAMRLARIGAVTYAY
                     SNEDGEPYGLSTAAIYDDLAKPFAEQAMDIRHLRVRLDGKSDLYADWRQRQGVNGH"
BASE COUNT          874 a         1563 c         1555 g          842 t
ORIGIN      
        1 gtagaaggtg acggcgatcc ccgtcgcggc gagcaaggca agccacagcc cggcgagaag
       61 gccgaaccag cgatgccaca gcttcagcgc gtggcgcggc cgccgccgga cagccgcgcc
      121 cgccatcaga agcggatgga agcggtcagg cggacgttgc ggccgggttc gggaaggccc
      181 gagacgatcg catagtcttc gagcccgagc tggccgtaat cggcgacgct cgcatgcgcg
      241 atatagcgct tgtcgaacag gttgtagacc gccgcctgca gttcgaggcg gtcgctgccg
      301 aacgggcgcc agctgccgaa gagatcgacc gtcgtatagc cgggcttgtc gatgaaacgc
      361 gtgccgtcga tgaagccgag ctggctttcg cggaacagca cctcgatatt gttgaggtcc
      421 tcgaaccgcg tcaggctggc gcggaagttg aaatagtcgc tcggctgata tcccgcggtc
      481 accgcccaat tgtcgcccat cgaattgccg agcgcaatat gctcatagcc ttcgatccgg
      541 ttgtcattga ggcgcgaatc ataatggttg taataaccgt cgatactgaa cggaccggcg
      601 cggtagccgg cgcgcagctc gattcccttc gatttgaact tgccgacatt ctcgtaatag
      661 accgcgtcct ggatcggccg cgcgtcgcgg tcgcggccga gttggtcgag gatgacgttt
      721 ttgatcgtca tctcgtaata gaccgccgac gcggtgaagc cgccgaggtc gtatttgacg
      781 ccgccctcga gattgtcgac gcgttcggga cgcagggccg gcgacatcga cagccacgcc
      841 gggttctttt cgagcgtgaa ggcgtcgccg atttccttgc cgcggaaggc ttcggcaaaa
      901 cccgcgttca ggctgaggcc ggggacgatc tcgaactcgg cgccgacgtt gaaactgagt
      961 ccgtcgctct tggtgccggt gcgataggtc gtctggtcga gatcataggc gtcgtagcgc
     1021 gcgccgaagc tgatcagcag cggatcgacg acgcgccaat ggtcctgaat atagacgccc
     1081 atcaacgcgc cctcttcggt gaagcggccg acgttcggat cccacgccca atcctgccac
     1141 atcgcgggat catcgagata ttcgctcgtc acgcgatcgc ggcggtgctc ggcgccgacg
     1201 gtgatgtcgt ggttgccgat cgcgaggttg gcgcgcgcgt cgaagccgta tgtttcgatc
     1261 tcggcgccat agaggcccca gcggtcgtcg cggttctggg tgaagttggt gcgcgtccag
     1321 taaccggtcg cctcgatacc gaccccgctg tcgccttcga agccatagtt gagcaccgcg
     1381 gtctggcgct tgcccttcgc ctcgaacagc cggtcgccgg cgagcacggg ccagttcgga
     1441 cgctggccga agcggccgct ttcgcggcgc tgttcgtagc tcgcggtaat ccggtggccg
     1501 ccgccgagct cgccgccgat cttgacgaag ccgaggttct gctcggcgcc ggtcgcgagc
     1561 tggcggacgc cgtcgccgtc cttgtacgga tcgcgattca catggacata ggaagcgagg
     1621 atgccgatgt cgcccgcgat gcggccatag agcgtgccgc tcaatttata gccgtcgttg
     1681 ctgaaccagc ccgccttggc gatgccgccg atgtcgcgcc cgtcctgcag catgtcgacc
     1741 gcattgcgcg tgcggaaacg gatcgcgccg ccgaccgcgc cgaagcccgc ggtcgcctcg
     1801 cccgggcccg cctgaacgtc gacggtttcg agcagttcgg gctcgatcgt aacgcggccg
     1861 atatggtgga acaaggtgcc gcgctgcggg gcgccgtcga tcgtcacgtt gagcagcgaa
     1921 tcctcgagcc cgcgcacata gattttctgc gcgagcccga cgccgccgcc gaccgaaacc
     1981 gagggaacat tgcggaacag gtcggcgagg tcgttcgcct ggaggacctc gatctcctcg
     2041 cggtcgatgg cgacgtcggt gagcgcgccg acgacgacga tgtcgcccgc ggcgtccgtg
     2101 tcgccttctt cggcgcgcgc ggcatggctc gtcagcgtga gcgccgcggc gcagctcgac
     2161 aggatcgcgg tacgaaattt catgaaagcc cccagcggtt aagatgcgtg cgcgccctaa
     2221 tcgctattgc gaactaaagt caacaaacgc tgcgattgaa aaggggccgc gagccgcgct
     2281 aggcaccgcc ggggacgagc aggagttgag ccgatgaccg agacgccgaa ccgcgaagcc
     2341 gcgcccgatg ccggggcgcc ggcgaatgtc gccgccaatc tcggatcgcg tttcctgcgc
     2401 tacctgcccc tcatcaccat cgcgcatttc gtcatcggcc tgcccgcgct gatcgcgtcg
     2461 ctcgcgctcg cctatttcgc gttcgtccag gccgatgcga cgcagaagat gcagaccggg
     2521 ggtgtgatgc ccttcgtcac cttcggaacg agcaatgggg acgaagatgg caatcaggat
     2581 atttcgctgt cgctgacgaa caatggcgtc ggccccgcga ttctcggccc cattgaaatc
     2641 cgttatgagg gcaagccgat caacaccccg gtcgagctgc tcaagacttg ctgcgcgaaa
     2701 accggaacgc agggcttgcg cctttccacc tcgccttcga ccggcatcgc cgtgcggccc
     2761 ggagaaacca tcgaattcgt gagctttccg cgcacccccg aatccgagaa ggtgtggcag
     2821 acgttcaaca gggagcggtg gaagctcaag gtccgcgcct gctattgctc gatcttcaac
     2881 gattgctgga tcaccgaagg aatgcaggga ttgccgaagc cggtgaacaa atgtcccgcc
     2941 gattggtcgc tctatcgcga ggacgcggga aaccggatcg cggggatcga agcgaaataa
     3001 ttcaaccaat cggttgacaa taaaatccgg ttctctcata ttcaaccaca tggttgaaca
     3061 tgattcgcac accctcgaca ccattttcca cgcgctcggc gacacgacgc gccgcgcgat
     3121 gctgggcgaa ctcgccgccg gcgaacggac ggtgggcgag ctcgccgaac cttttgcgat
     3181 gtcgctcgcc gcggcgtcga agcatatcaa agtgctcgaa gccgccgggc tggtccgccg
     3241 cgacatccgc ggccgcacgc atgtctgcag cctcgatccc gtcccgctga tgcgtgcgga
     3301 ccagtggatc ggcatctacc gccgcttctg gaccgatcgc ctcgacgtgc tggagaaaat
     3361 ccttcgcgcc gacgatgccg cgccccctcc cccaagaaac ccctccccgg gaaagaagga
     3421 gaaacatgat gacgacaatg cttgacgaag gccgctacgg cctgctcacc gaacccgcga
     3481 cgctgacgat cacccgcctg ctccccggcc cgatcgaccg catctgggcc tatctcaccg
     3541 acggcgacat gcgccgccaa tggctcgccg cgggcgcgat ggaggaacgg gccggcgcac
     3601 cgatcgagct tgtgtggcgc aacgacgaac tcaccgatcc gccgggcaca cgccccgaag
     3661 gctttggcga cgagcaccgg atggcgtgcg aagtcgtcgc ggtcgacgcg ccgcgcctct
     3721 tgtcgattag ctggggcagc accggcggcg tgaccttcac gctcgaggag aagggcgacg
     3781 aggtgttgct gacgatcgtc cacaggcgcg tcgaggatcc cgccgtgctg ctcaatgtca
     3841 gctcgggctg gcacgcgcat ctcgacgtgc tcgaagcgcg cctgcgcggt acccaggccg
     3901 aaccatactg ggacaattgg acccggctgc gcagcgaata tgccgagcgg ctgttcggct
     3961 gactttttcc cgaagcggct gcccgcatgg acagtcggcc ggcgatcgtg gcatcctgac
     4021 ggcacactcg gccgaaacgg atgccacgat catggacgac agcgaaacat atctgcgtga
     4081 ggcgatcgcg ctcgcctatg ccaacgccga aaagggcggc cgtccgtttg gggcggtgat
     4141 cgtccgtgac gggcgggtca tcgccagcgc ggtgaacgag attttggcga ccggcgaccc
     4201 gacatcgcac gccgagctca acgcgatccg gctggccagc aaagtggtcg gatcgcccga
     4261 tctggccggc tgctcggtgt tcgcgagcgg ccacccctgc ccaatgtgca tggcggcgat
     4321 gcgcctcgcg aggatcggcg cggtgaccta tgcctattcg aacgaggatg gcgagcctta
     4381 cggtctgtcg acggcggcga tctacgacga tctcgccaag ccctttgccg agcaggcgat
     4441 ggatatccgc catctgcgtg ttcgtctcga cgggaagtcc gacctctatg ccgactggcg
     4501 gcagcggcag ggtgttaacg gacattgaga aatatgcgcc agcccctcct cttcagagga
     4561 ggggtagcga gacttacgaa cttgttcgtt agtcgcagcg gggtggtcgt cgtggccagg
     4621 cgcgcgcgtg ccgccaccac caccccgttc gactaaaggc ccggctgcgc cggaccaagt
     4681 ctcactgccc ctcctctgaa gaggaggggt tttgggcaac gtccgctccc caccccaaag
     4741 ccgacaaaaa aggggccgcc tttcggcagc cccttctctt ttccggtagc ggaagacctt
     4801 agtccttcag gaagtcgggc acatggccct gatc
//
LOCUS       DATMSU010000356         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_120190,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000356 DATMSU010000000
VERSION     DATMSU010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120190"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1794
                     /locus_tag="VN034_09595"
     CDS             163..1794
                     /locus_tag="VN034_09595"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWV60897.1"
                     /translation="MARFIFITGGVVSSLGKGLMAASLAALLQARGYRVRIRKFDPYL
                     NVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTGVAARQSDNVTSGRIYQGIIAKE
                     RRGDYLGATVQVVPHVTDAIKEFARAETDDLDFVLCEIGGTVGDIESLPFIEAIRQLK
                     NEVGRENAISVHVTLVPYIAAAGELKTKPTQHSVRELASLGVQPDILLCRCEKPLPEN
                     ERAKIAQFCNVRKEAVIPALDADSIYSVPVQYHGEGLDSEVLHAFGIHDAPAPDLSRW
                     YDIMDRKQHPEGEVTIGVVGKYVSLPDAYKSLNEALVHGGMAHRVKVNIRWLDAEMFE
                     RDEDLAANLEPMHGILVPGGFGERGSEGKISSVRFARERGVPFFGICLGMQMACIEGA
                     RNTSGIDDASSTEFGPTGEPVVGMITEWMSDEGLQKRGADTDLGGTMRLGAYEAKLSP
                     NSHVASVYGANDISERHRHRYEVNNAYREQLEKGGLVFSGMSPDGMLPEIVERPDHPW
                     FIGVQFHPELKSKPFDPHPLFAGFIEAAVKQSRLV"
     gene            1821..2504
                     /locus_tag="VN034_09600"
     CDS             1821..2504
                     /locus_tag="VN034_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWV60898.1"
                     /translation="MDHAKLAELQGPDSIFSGLTIEDWAEIARRAVQVNFVKGKELLV
                     QGDPGDMMLILTEGTARVSMLTSGGREIVLAYAEPGAVLGEIALLDGGERTASVTATS
                     AGSALQLGRNALKDFAASHPEFAWSLMQQLARRLRTADQTIESDRAYASGPRLARYLK
                     RLIRKDIDASQRVELSQTELGNFAGMSREHINRQLKSWEESGVISLEQGRVRVLDAEM
                     LEDISESEG"
BASE COUNT          501 a          847 c          855 g          462 t
ORIGIN      
        1 atcgagccag gaagccgctc cggctcccgc acaggcaccc cccgccaaga aataaccggc
       61 ggcttcggcc tccgacccaa cccgcccggc ggaaatattt tccgccggat gcgattcgac
      121 ggcttgcgcc gtccccctcc cgttgagtaa gtctttcctc ccatggcgcg gttcattttt
      181 atcaccggcg gcgtggtctc ctcgcttggc aaaggtttga tggcggctag cctcgcagcc
      241 ttgttgcagg cgcgtggcta tcgtgtccgt atccggaaat tcgatcccta tctgaatgtc
      301 gatccgggca cgatgtcgcc ctatcagcac ggcgaggtct atgtgaccga cgacggcgcc
      361 gaaaccgacc tcgacctcgg ccattatgag cgtttcaccg gcgttgccgc gcggcagagc
      421 gacaatgtca cctcgggccg tatctatcag ggcatcatcg ccaaggaacg ccgcggcgac
      481 tatctgggtg cgacggtgca ggttgttccg cacgtcaccg acgcgatcaa ggaattcgcg
      541 cgcgccgaga ccgacgatct ggatttcgtg ctctgcgaga tcggcggaac ggtcggcgac
      601 atcgaatcgc tgcccttcat cgaggcgatc cgccagctca agaacgaggt cggacgcgaa
      661 aacgcgatct cggtccatgt cacgctggtg ccctatatcg ccgccgcggg cgaattgaag
      721 accaagccga cgcagcacag tgtgcgcgaa ctcgcgtcgc tcggcgtcca gcccgacata
      781 ttgctctgcc gctgcgaaaa gccgctgccc gagaatgagc gcgcaaaaat cgcgcaattc
      841 tgcaacgtcc gcaaggaagc ggtgattccg gcgctcgacg ccgacagcat ctattcggtc
      901 ccggtccagt accatggcga agggctggac agcgaagtgc tgcacgcctt cggcatccac
      961 gatgcgcccg cacccgactt gtcgcgctgg tacgacatca tggaccgcaa gcaacacccc
     1021 gaaggcgagg tcacgatcgg cgtcgtgggc aaatatgtgt cgctgcccga cgcgtataaa
     1081 tcgctcaacg aagcgctggt ccacggcggc atggcgcacc gggtcaaggt caatatccgc
     1141 tggctcgacg ccgaaatgtt cgagcgcgac gaggatctcg ccgccaacct cgaaccgatg
     1201 cacggcatcc tcgtccccgg cgggttcggc gagcgcgggt ccgaaggcaa gatttcgagc
     1261 gtgcgttttg cgcgcgaacg cggcgtgccc ttcttcggca tctgcctcgg catgcagatg
     1321 gcgtgcatcg agggcgcgcg gaacaccagc ggcatcgacg atgcctcaag caccgaattc
     1381 ggcccgactg gcgagcccgt cgtcggcatg atcaccgaat ggatgagcga cgaaggcttg
     1441 cagaaacgcg gcgccgacac cgatctgggc ggcacgatgc gcctcggcgc ctatgaagcg
     1501 aagctgtcgc cgaacagcca tgtcgcgagc gtctatggcg ccaacgacat cagcgagcgc
     1561 caccgccatc gctacgaagt caacaacgcc tatcgcgagc aactcgaaaa gggcgggctc
     1621 gtcttttcgg gcatgtcgcc cgacggcatg ttgccggaaa tcgttgagcg cccggaccat
     1681 ccgtggttta tcggggtgca gttccacccg gaactcaagt cgaagccgtt cgatccgcat
     1741 cctttgttcg cgggattcat cgaagcggcg gtgaagcaga gccggctggt ttagaacgac
     1801 gcaaaggggt gcaggggcag atggatcacg cgaaactcgc cgagttgcag ggccccgaca
     1861 gcatcttttc aggcctgacg atcgaggatt gggcggaaat cgcgcgccgc gccgtgcagg
     1921 tcaatttcgt caagggcaag gaattgctcg tccagggcga cccgggcgac atgatgctga
     1981 tcctgaccga aggcaccgca cgcgtgtcga tgctgacctc gggcggccgc gaaatcgtgc
     2041 tcgcctatgc cgaaccgggc gcggtgctcg gcgagatcgc gctgctcgac ggcggcgagc
     2101 gcaccgcgtc ggtgaccgcg acgagcgccg ggagcgcgct gcagctcgga cgcaacgcgc
     2161 tcaaggattt tgccgcgagc catcccgaat tcgcctggtc actgatgcag cagctcgcgc
     2221 ggcggctgcg caccgccgac cagacgatcg agagcgaccg cgcctatgcc tcgggcccgc
     2281 ggctcgcgcg ctatctgaaa cggctgatcc gcaaggatat cgacgcatcg cagcgcgtcg
     2341 aactcagtca gaccgaactc ggcaatttcg cgggcatgag ccgcgagcat atcaaccgcc
     2401 agctcaagag ctgggaggaa tcgggcgtga tctcgctcga acaggggcgc gtgcgcgtgc
     2461 tcgatgcgga gatgctcgag gatatcagcg agagtgaggg atagcgggcg gcttgaccgt
     2521 tttcggccgt gtgtggacgg ccctccgttg gcaagtggtt tctggatgtg atgcgacctg
     2581 ctggttggtg cagccatgtg tccggcctgt gatgcggcct tgtcatgcgc cgctggccat
     2641 gatgcctttc gcgcggatcg ggtcc
//
LOCUS       DATMSU010000357         6164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_72615, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000357 DATMSU010000000
VERSION     DATMSU010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72615"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1971)
                     /locus_tag="VN034_09605"
     CDS             complement(<1..1971)
                     /locus_tag="VN034_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV60899.1"
                     /translation="MRILLIAALLASAAPVTVHAHEAPAPSAESKPVPKEELLKPPAD
                     AAHYVVVSEAGKHGDQWRWQLSDGRTAYRWSQELRGWITEMDQVTTFGPGGTIDALTV
                     RGVTMSGDAAEEYRVANGRATWKTANDSGEAPAGGWYIPAGGVGIANAPLIDALAAAG
                     DAGLDFLPSGKGHMSFGPTQVIQGPGGPKTVQLAFVSGILPSPLPVWLDENKRHFADI
                     SYISVIPAGYEGALKQLRDVQEKATADAVAGVAKRFLTPAAKAPVLFDNVKLFDADKG
                     TFLASRAVLAQDGKIAAIGAAGSLKAPAGTRVIDGRGKTLVPGIWDSHLHIGDDWDVL
                     SNIANGITSFRSPGTTFDRAVDATKRRAEGSLLMGEPFISVIIDKKDPLAAQGAEVVS
                     SVEEAIAAVRRVKEAGLWGIKFYTSMNPAWIAPAAAEAHRLGLHVHGHVPATMKPSEA
                     VAAGYDELTHLNFVVMESMPREVIDKSNTRARMEGPARYFKDVDLDAPLMKGFIADLA
                     AKKTLVDPTIVIFEGLFTQDGGKPHPAYAPYMGIISPVIERSVFTSGGYPLIEGLTRD
                     DYRKSYAKMVELVGRLHKAGVPIVAGTDGWGIELIRELEIYRQAGFTPAEAIQSATIV
                     PARVVGADKRTGSIAVGKEADMVLVDGDAATDL"
     gene            complement(2094..2402)
                     /locus_tag="VN034_09610"
     CDS             complement(2094..2402)
                     /locus_tag="VN034_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HWV60900.1"
                     /translation="MTAPTAFKVLTQQQWADFERERVFRGAPVDIADGYIHLSTAEQL
                     DATIAKYFADQSGLMIAEVDLILLGEAIRWEPARGGDLFPHIYAELPIHAVISLQKRD
                     "
     gene            complement(2399..5125)
                     /gene="gyrA"
                     /locus_tag="VN034_09615"
     CDS             complement(2399..5125)
                     /gene="gyrA"
                     /locus_tag="VN034_09615"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWV60901.1"
                     /translation="MQPSDDGVSPINIVDEMKTSYLDYAMSVIVSRALPDVRDGLKPV
                     HRRILYAAQEGGFIPGRPYKKSAKIVGDVMGNYHPHGDSAIYDALARMTQDWSLRVPL
                     IDGQGNFGSMDPDPPASMRYTEARLAKTAMVLLNDLDKDTVDFQPNYDASRDEPTVLP
                     ARFPNLLVNGAGGIAVGMATNIPPHNLGEVIDATLAMIDRQLEGGPPVTLEELMAIVP
                     GPDFPTGAMMLGQGGARNAYATGRGSIMMRATHIIEEGRNDRQSIVLTSIPFQVGKSG
                     LVEKIAEAARDKRIEGVADIRDESNREGVRVVIELKRDATAEVVLNQLWRHTPAQSSF
                     PANMLAIRGGRPEMLGLQDILTAFIAFREEVITRRCKFELAKARDRAHILLGLVVAVS
                     NLDEVVRIIRGSPSPAAAREALLAREWPIGEIAPYIRLVEAIEGEMEESATYRLSEIQ
                     VKAILDLRLHRLTALGRDEIGKELGELAAEIEELLSILADRVKLYGVMREELVAVREE
                     FATPRRTLLAPAADGIDDEDLIEREEMVVTVTLDGYIKRTPLDTFRAQRRGGKGRAGM
                     ATKDEDVVTELFVTSTHTPVLFFSTAGKVYRMKVWRLPEGGPATRGRPMINLLPLAQG
                     ETISTVLPLPEDEAEWGKLHVMFATAKGSVRRNSMDAFTNVPSNGKIAMKFEGDDEDD
                     RLIGVALLDESDDVLLATRQGKAIRFAGDDVREFQSRNSTGVRGMRLADGDAVISLSI
                     LHRVGTSSEEREAYLRAAPWKDNENAPTLDGERMAELAGREQFILTVCANGYGKLSSA
                     YEYRRTGRGGQGITNIDNIGRNGPVVASFPATKVHQLMLVTDQAKLIRMGLDSMRVIG
                     RGSAGVRLFDVAKDEHVVSAALIEESDEEDVEGEGEAGAAPESETPSEAAPE"
     gene            5382..>6164
                     /locus_tag="VN034_09620"
     CDS             5382..>6164
                     /locus_tag="VN034_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60902.1"
                     /translation="MALIALVGASETLPDGSLRALVTVAGETLIERQAARLADVGVTH
                     IAVAVAAVPAELLATCDRIRRRGIKVTPVRAASDLVPLVEADDRIILVADGLRASGTH
                     YAAIAKPGSPAILVTGDTMLTQAFERIDATRRWGGLASISQPMVAELAGMPGEWDMML
                     TLLRLAVQAGARRLYCEPALFEQGEIAIVADRPTAALIEQSSLQQVEYGGMGLGRSAI
                     MMPVIRLAGPLLVKSPATARYLPHITALLWVAVALLAASGLAA"
BASE COUNT          948 a         2005 c         2100 g         1111 t
ORIGIN      
        1 gaggtcggtc gcggcgtcgc cgtcgacgag caccatgtcg gcttccttgc ccaccgcgat
       61 cgagccggtg cgcttgtcgg cgccgacgac gcgcgcgggg acgatggtgg cgctctggat
      121 cgcctcggcc ggcgtgaaac cggcctgccg gtagatttcg agttcacgga tcagctcgat
      181 cccccagccg tcggtgccgg cgacgatcgg cacgccggcc ttgtgcagcc ggccgaccag
      241 ttcgaccatc ttcgcatagc ttttgcggta atcgtcgcgc gtcagcccct cgatcagcgg
      301 atagccgccc gaggtgaaca ccgaacgttc gatgacgggc gagatgatgc ccatataggg
      361 ggcataggcc ggatgcggct tgccgccgtc ctgcgtgaac agcccttcga aaatgacgat
      421 cgtcggatcg acgagggtct tcttcgccgc gaggtcggcg atgaagcctt tcatcagcgg
      481 cgcgtcgagg tcgacatcct tgaaatagcg cgccggccct tccatccgtg cccgcgtgtt
      541 cgacttgtcg atcacctcgc gcggcatcga ttccatcacg acgaaattga ggtgggtcag
      601 ctcgtcatag ccggcggcga ccgcctcgct cggcttcatc gtcgcgggga cgtggccgtg
      661 gacatggagg ccgagcctgt gcgcctcggc ggcggcgggc gcgatccacg cggggttcat
      721 cgaggtgtag aatttgatgc cccagaggcc cgcttccttg acgcggcgca cggcggcgat
      781 cgcctcttcc acgctgctga ccacttcggc gccttgcgcc gcgagcgggt ccttcttgtc
      841 gatgatgacc gagatgaagg gctcgcccat caacaaactg ccttcggcgc ggcgcttcgt
      901 cgcgtcgacc gcgcggtcga aggtcgtgcc ggggctgcgg aagctggtga tcccgttcgc
      961 gatgttcgag agcacgtccc aatcgtcgcc gatatgaaga tggctgtccc agatgccggg
     1021 caccagcgtc ttgccgcgcc cgtcgatcac gcgcgtcccg gcgggggctt tcagcgagcc
     1081 ggcggcgccg atcgcggcga tcttgccatc ctgcgcgagc accgcgcgcg atgcgaggaa
     1141 ggtgcccttg tcggcgtcga acagcttgac attgtcgaac agcaccggcg ccttggcggc
     1201 gggggtgagg aagcgtttgg cgacgccggc gaccgcatcg gccgtcgcct tttcctgcac
     1261 gtcgcgaagc tgtttcagcg cgccttcata gcccgccggg atcaccgaga tatagctgat
     1321 gtcggcgaag tggcgcttgt tctcgtcgag ccagacggga agcggcgagg ggaggatgcc
     1381 gctgacgaag gcgagctgga ccgtcttggg gccgcccggg ccctgaatca cctgcgtcgg
     1441 tccgaaactc atatggccct tgccgctggg caggaaatcg agtccggcat cgcctgcggc
     1501 ggcgagcgcg tcgatcagcg gagcgttcgc gatgccgacc ccgcccgccg gaatatacca
     1561 gccgcccgcg ggcgcttcgc ccgaatcatt cgcggttttc cacgtcgcgc ggccgttcgc
     1621 gacgcgatat tcctcggccg cgtcgcccga catggtcacg ccgcgcacgg tcagcgcatc
     1681 gatcgtgccg ccggggccga aggtcgtcac ctgatccatt tcggtgatcc agccgcgcaa
     1741 ttcctgcgac cagcgatagg cggtgcggcc gtcggaaagc tgccagcgcc actggtcgcc
     1801 atgcttgccg gcttcggaga cgacgacata atgcgccgcg tcggcgggcg gcttgagcaa
     1861 ttcttccttg gggacgggct tcgattccgc cgaaggtgcg ggcgcttcat gcgcgtggac
     1921 ggtgacgggt gcggcggacg ccagcagggc ggcaatcaac aggatgcgca tcttctctct
     1981 tccccaaaga acggacggcg taaggtccga caatggtggg gaatttcagc gcgcgaagca
     2041 agatgcgacg aacacgcgcg cgcgttcgac caatgcgctc cgagctattg gcctcagtcg
     2101 cgcttttgca ggcttattac cgcgtggatc ggcagttccg catagatatg gggaaagagg
     2161 tcgccgccgc gcgccggctc ccagcggatc gcctcgccga ggaggatgag gtccacctcg
     2221 gcgatcatca ggcccgactg atcggcgaaa tatttggcga tcgtcgcgtc gagctgctcg
     2281 gcggtcgaca ggtggatata gccgtcggcg atatccaccg gggcgccgcg gaagacgcgc
     2341 tcgcgctcga aatcggccca ttgctgctgg gtcagcacct tgaaggccgt gggtgcggtc
     2401 attcgggcgc cgcctcgctc ggcgtttcgg attcgggcgc tgcgcccgcc tcgccttcgc
     2461 cttcgacgtc ttcctcgtcg ctttcctcga tcagcgccgc cgagacgaca tgttcgtcct
     2521 tggcgacgtc gaacaggcgc acccccgccg agccgcgtcc gatgacgcgc atcgaatcga
     2581 ggcccatccg aatgagcttt gcctggtcgg tcacgagcat caactgatgg accttggtcg
     2641 cggggaagct ggcgaccacc gggccgttgc ggccaatatt gtcgatgttg gtgatcccct
     2701 ggccgccgcg gccggtgcgg cgatattcat aggccgacga cagcttgcca tagccgttgg
     2761 cgcagacggt gaggatgaac tgctcgcgcc cggcgagttc cgccatgcgt tcgccgtcca
     2821 gcgtcggcgc gttctcatta tccttccacg gcgcggcgcg gagataggct tcgcgttcct
     2881 cgctgctcgt gccgacgcgg tgaaggatcg agagcgagat caccgcgtcg ccgtcggcga
     2941 ggcgcatgcc gcgcaccccg gtcgaattgc ggctctggaa ctcgcggaca tcgtcgcccg
     3001 cgaagcggat cgccttgccc tgccgcgtcg cgagcaggac atcgtcgctt tcgtcgagca
     3061 gcgcgacgcc gatcagccga tcgtcctcgt cgtcgccctc gaacttcatc gcgatcttgc
     3121 cgttcgaggg cacgttggtg aacgcgtcca tgctgttacg acgcacgctg cccttcgcgg
     3181 ttgcgaacat gacgtgcagc ttgccccatt cggcctcgtc ctcgggcagc ggaagcacgg
     3241 tcgagatcgt ctcgccctgc gccagcggca gcaggttgat catcgggcgg ccgcgcgtcg
     3301 cgggtccgcc ctcgggcagg cgccacacct tcatgcggta aaccttgcct gcggtcgaga
     3361 agaagaggac gggcgtgtgc gtcgaggtga cgaacagctc ggtgacgaca tcctcgtcct
     3421 tcgtcgccat gccggcgcgg cccttgccgc cgcggcgctg ggcgcggaag gtatcgagcg
     3481 gggtgcgctt gatatagccg tcgagggtga cagtgacgac catctcctcg cgctcgatca
     3541 gatcctcgtc gtcgatgccg tcggcggcgg gggcgaggag cgtgcggcgc ggggtcgcga
     3601 actcctcacg caccgcgacc agctcttcgc gcatcacgcc atagagtttg acgcggtcgg
     3661 caaggatcga aagcagttcc tcaatctcgg ccgccaactc accgagttcc ttaccgattt
     3721 catcgcgccc gagcgcggtg aggcggtgga ggcgcaggtc gaggatcgcc ttcacctgga
     3781 tttcggagag gcgataggtg gcgctttcct ccatctcgcc ctcgatcgct tcgacgaggc
     3841 ggatataggg cgcgatctcg ccgatcggcc attcgcgcgc gagcagcgct tcgcgcgcgg
     3901 cggcgggcga gggcgaaccg cggatgatgc ggacgacctc gtcgagattc gacaccgcga
     3961 cgacgaggcc gagcaagatg tgcgcgcggt cgcgcgcctt cgcgagttcg aacttgcagc
     4021 ggcgggtgat gacctcttcg cggaaggcga tgaaggcggt gaggatgtct tgcagcccca
     4081 gcatttcggg gcggccgccg cggatcgcga gcatgttcgc agggaagctc gactgcgccg
     4141 gcgtgtggcg ccagagctgg ttgaggacga cttccgccgt cgcatcgcgc ttcaactcga
     4201 tgacgacgcg cacgccctcg cggttcgatt cgtcgcgaat gtcggcgaca ccctcgatgc
     4261 gcttgtcgcg cgcggcttcg gcgatcttct cgacgagccc cgacttgccg acctggaagg
     4321 gaatcgacgt cagaacaatc gattgacggt cgttcctgcc ttcctcgatg atgtgcgtcg
     4381 cgcgcatcat gatcgagccg cgacccgtgg cataagcgtt gcgtgcaccg ccctggccga
     4441 gcatcatcgc gccggtcggg aagtcgggac cggggacgat cgccatcagc tcctcgagcg
     4501 tgaccggcgg gccgccttcg agctgacggt cgatcatcgc gagcgtcgcg tcgatcactt
     4561 cgccgagatt gtgcggcggg atgttcgtcg ccatgccgac cgcgatgccg cccgcgccgt
     4621 tgacgagcag gttggggaaa cgcgccggga gcaccgtcgg ctcgtcgcgg ctcgcgtcat
     4681 aattgggctg gaagtcgacg gtgtccttgt cgagatcgtt cagcagcacc atcgcggtct
     4741 tcgcaaggcg cgcttcggtg taacgcatcg acgccggcgg atcggggtcc atcgagccga
     4801 aattgccctg accgtcgatc agcggcacgc gcagcgacca atcctgcgtc atacgcgcga
     4861 gcgcgtcgta gatcgcgctg tcgccatgcg ggtgatagtt acccatcacg tcgccgacga
     4921 tcttcgccga tttcttgtac gggcggcccg gaatgaagcc gccttcctgc gccgcgtaga
     4981 ggatgcggcg gtgcaccggc ttcagcccgt cgcgcacgtc ggggagcgcg cggctgacga
     5041 tcacgctcat cgcgtaatcg aggtaggacg tcttcatttc gtcgacgata ttgatcggcg
     5101 aaacgccgtc gtcggacggc tgcataggcg tattttcttc ggtcaagacc cggccttttt
     5161 ctgctctttc gggagatgga tttggtctag cccatgggcc cgggaaagac cagcgattcg
     5221 gtccttttgc cgggattttt ttgcaggcgg aaaaggcagg gaaagcgcga taaaccaagg
     5281 tgaaggcgtg acgtcatcgt ggcgcatcgc ggggcttgcc aatcgcttcg ccgggcctaa
     5341 agcttcaccg cgatgaccgc tgccgataaa tcctggcgcc gatggccctg attgcgctgg
     5401 tcggcgcttc cgaaacgctg cccgacgggt cgctgcgcgc gctcgtcacg gtcgccggcg
     5461 agacgctgat cgagcgtcag gcggcgcgcc tcgccgatgt cggggtgacg catatcgcgg
     5521 tcgcggttgc cgcggtcccc gccgaactgc tcgcgacctg cgaccgcatt cgccgccgcg
     5581 ggatcaaggt gaccccggtg cgcgcggcaa gcgatctcgt cccgctggtc gaggccgacg
     5641 accggatcat cctcgtcgcc gatggcctgc gcgcgagcgg cacccattat gccgcgatcg
     5701 cgaagcccgg atcgcccgcg atcctcgtca ccggcgacac gatgctgacg caggcgttcg
     5761 agcggatcga cgcgacgcgg cgctggggcg gtctcgcctc gatctcgcag cccatggtcg
     5821 ccgaactcgc aggcatgccg ggcgaatggg acatgatgct gacgctgctg cgcctcgcgg
     5881 tgcaggcggg cgcgcggcgg ctctattgcg aaccggcgct gttcgaacag ggtgagatcg
     5941 cgatcgtcgc cgatcgcccg accgcagcgc tgatcgagca atcgagcctg caacaggtcg
     6001 aatatggcgg catggggctg gggcggtcgg cgatcatgat gccggtcatc cggctggcgg
     6061 gaccgctgct tgtcaaatcg ccggcgaccg cgcgctatct gccccatatc accgcgctgt
     6121 tgtgggtggc ggtcgcgctg ctcgcggcga gcggactcgc ggcg
//
LOCUS       DATMSU010000358         6673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_158118,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000358 DATMSU010000000
VERSION     DATMSU010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158118"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2541)
                     /locus_tag="VN034_09625"
     CDS             complement(<1..2541)
                     /locus_tag="VN034_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWV60903.1"
                     /translation="MGIRRAIIGPGAGDPTTGESLFDSPEFVTAVMRVAKLWHYVLIG
                     RVLAFLSFAFLPGAAGFLDHLSHWLVMAAGLPCDVALMVIGQAMRRPRPIAHQRVRTM
                     AILCMALIALGTLLNAAAMFAAIAVPDEGSYFDAFTAIHVGSLLVAASAAAIVRPAFF
                     TFAGATVLGGAIGVASWPFGVAGFIFLGLLIAMMREDVRHQRRATRAARLAIGDQQRA
                     LNLMRDFERAGRGWFWETDRNGRLVYISPTVAARLDRPLAHLLGHPFTDIIRKRIGKD
                     ESEERTLGFSLSSRTPFKELTVQAAVPGEERWWSISGQPISNEFGNFQGFRGSGTDLT
                     DQKRSEREINQLARYDTLTGLANRRHITDLLERALKSHSGQPQPCALLLMDLDRFKAV
                     NDTLGHPVGDQLLQQVAGRLTQIIGDKGQVGRLGGDEFQIVVPQMSQPEKLAGIANAI
                     ILSLAKPFAIEGEQVRIGSSIGIAVSEGQGVSASALVRNADLALYAAKDAGRGVYRFY
                     ADAMHNQASERKAIEDALRDALAKDELQLLYQPIVDVTSERISGFEALIRWHHATAGT
                     ISPSKFIPIAEESNLIVPIGEWIIRSACAAIARLGPGYRVAVNVSPRQFANEKLPATI
                     VNAVSAAGIRPEQLELEITEGVFLDESAENLAMFQKLKRTGVRLALDDFGTGYSALGY
                     LKKAPFDKIKIDQSFVLGAADKNSMNAAIISSIVGLATALDMETTAEGVETHDDLALI
                     RGLGCSHVQGYIYGRPMDLAEVLDMLREGGGRVEAKGFKSAREARLTVFRTIQIESGG
                     HRYEAIVRNMSSRGALIEGLWNVPPGTPLTLEFGPGQPVAAEARWSAGN"
     gene            2689..3720
                     /locus_tag="VN034_09630"
     CDS             2689..3720
                     /locus_tag="VN034_09630"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HWV60904.1"
                     /translation="MPAVRLFPPDRFFDRAEWSAITRASSWRGIWLVAHAWIVSIALV
                     GLAAWSQNPVAWLLAVIFVGGRQLGLAILMHDAAHGALHPNRKINNFLGQWLTGAAVG
                     SDLIAYRTYHLQHHKFTQQPEDPDLVLSKPFPTTRASLRRKMLRDLTGQTFFKQRMAQ
                     FTFAFAGLKAMLRGEAVQKGGASTKAGTPFNKQSDDGMTSPTIDIAGAMAVTRAVGRF
                     LIVQAVLLALSLALYGWTPYLLWLAGLATTFQLYLRIRNIAEHACTTTGSDDPFTHAR
                     TTDAGWLARATVAPYWVNYHAEHHLFMGVPCYRLPEVHAALGRAGKHDMMTIAPNYAA
                     VLRRVTAKG"
     gene            complement(3902..4390)
                     /locus_tag="VN034_09635"
     CDS             complement(3902..4390)
                     /locus_tag="VN034_09635"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HWV60905.1"
                     /translation="MSDVIDIFTTGGTIDKVYFDALSEFQIGPAAIPDMLRENNVHVA
                     HRVTQLMRKDSLELGDADREAIRAAVAASDASRILVTHGTDTMVVTGRVLAGIEGKTI
                     VMTGAMQPASVRASDAEFNVGFALAAVQTLPPGVYVAMNGMIFDPEKTVKDRAGARFV
                     EE"
     gene            complement(4387..5988)
                     /locus_tag="VN034_09640"
     CDS             complement(4387..5988)
                     /locus_tag="VN034_09640"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWV60906.1"
                     /translation="MTDLHLHPSLREPAQTSKAWPFEAARKLVKRYPDGKQGGEPVLF
                     ETGYGPSGLPHIGTFNEVLRTTMVRRAYEALTGGAPTRLVAFSDDMDGLRKVPDNVPN
                     GDMLREYLGKPLTAIPDPFGKFESFAHHNNAMLREFLDRFGFEYEFVSSTEQYKAGAF
                     DAALKNVLRHNQDILDIMLPTLRAERAATYSPILPVSPKSGIVLQVPVTVVDADAGLV
                     SFEDEGETVTHSVLGGGAKLQWKVDWAMRWVALGVDYEMYGKDLTDSGVQSGKIAKAL
                     GGRKPEGLIYEMFLDEKGEKISKSKGNGLSIEEWLSYGSEESLAFFAFREPKAAKQLH
                     IGVVPKAVDEYLQMRGNYPAQEPDKKLGNPVHHVHVARGQDVPRAELPVTFGLLLNLV
                     GVMGADASKAQIWRYLGQYVAGADATSYPELDRLIDNAMAYNRDFVAPTLKRRKPVGG
                     EGAALKELDAKLAALPAEASADDIQNIVYEIGKNEAYGFENLRDWFKALYETLLGSSA
                     GPRMGSFIALFGIDNTRRLIAEALA"
     gene            6095..6463
                     /locus_tag="VN034_09645"
     CDS             6095..6463
                     /locus_tag="VN034_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV60907.1"
                     /translation="MDNFRLPAPRGHKRTPVSIDVTVNGILNFVDGRIADLSEGGARI
                     DGASMPARSRCEIHYGGQVTYAVVMWSEHDRMGVRFPYELAQGALYNALQSARNAAMI
                     EPAQIFHSNRPAVFGRRGLS"
BASE COUNT         1137 a         2330 c         2042 g         1164 t
ORIGIN      
        1 gttgcccgcc gaccagcgcg cttcggcggc gaccggctgg ccgggcccga attcgagcgt
       61 cagcggcgtt cccgggggca cgttccacag cccctcgatc agcgcgccgc gcgacgacat
      121 gttgcggacg atcgcctcgt aacgatgtcc gccgctttcg atctgtatgg tgcgaaagac
      181 cgtcagccgc gcctcgcgcg cgctcttgaa ccccttggcc tcgacgcgcc cgccgccctc
      241 gcggagcatg tcgagcactt cggcgaggtc catcggccgc ccatagatat agccttggac
      301 atggctgcat ccgagcccgc ggatcagcgc gagatcgtcg tgcgtctcga ccccctcggc
      361 ggtggtctcc atgtcgagcg cggtcgccag cccgacgatc gaagagatga tcgcggcatt
      421 catgctgttc ttgtcggcgg cgcccagaac gaagctctgg tcgatcttga tcttgtcgaa
      481 cggcgccttc ttcagatagc cgagcgccga atagccggtg ccgaaatcgt cgagcgccag
      541 ccgcacgccg gtgcgtttca gcttctggaa catcgccaga ttctcggcgc tttcgtcgag
      601 gaagaccccc tcggtgatct cgagttcgag ttgctcgggc cggatgcccg ccgccgacac
      661 cgcgttcacg atcgttgccg gcagcttttc gttcgcgaac tggcgcggcg agacgttcac
      721 cgcgacgcgg tagccgggac cgagccgggc gatcgcggcg caggccgaac ggatgatcca
      781 ttcgccgatc gggacgatca ggttcgattc ctcggcgatg ggaatgaatt tcgacgggct
      841 gatcgtgccc gcggtcgcat gatgccagcg gatcagcgcc tcgaaccccg aaatgcgctc
      901 gctcgtgaca tcgacgatcg gttggtagag caattgcagc tcatccttcg ccagcgcgtc
      961 gcgcagcgcg tcctcgatcg ccttgcgctc gctcgcctgg ttgtgcatcg catcggcata
     1021 gaagcgatag acgccgcgcc ccgcatcctt cgcggcatag agcgcaaggt cggcattacg
     1081 gacgagcgcc gatgcgctca ccccttgccc ctccgaaacc gcgatgccga tcgacgatcc
     1141 gatgcgcacc tgctcgccct cgatcgcgaa gggcttggcg aggctcagga tgatcgcatt
     1201 ggcgatgccc gcgagcttct cgggctggct catctgcggc acgacgatct ggaactcgtc
     1261 gccgccgagc cgcccgacct gccccttgtc gccgatgatc tgcgtcagcc gtcccgcgac
     1321 ctgctgcaac aactggtcgc cgaccggatg ccccagcgtg tcgttcaccg ccttgaaccg
     1381 gtcgagatcc atcagcagca gcgcgcacgg ctgcggctgg ccgctatggc tcttcagcgc
     1441 gcgttcgagc aggtcggtga tgtggcgccg gttcgcaagg ccggtcagcg tgtcatagcg
     1501 tgcgagctgg ttgatctcgc gctccgacct cttctggtcg gtcaggtcgg tgccgctgcc
     1561 gcgaaagccc tgaaaattgc cgaactcgtt gctgatcggc tggcccgaga tcgaccacca
     1621 gcgctcctcg cccggcaccg ccgcctgcac cgtcagctcc ttgaacggcg tgcgcgacga
     1681 caggctgaac cccaatgtgc gctcctcgct ctcgtccttg ccgatgcgct tgcggatgat
     1741 gtcggtaaag gggtggccga gcagatgcgc gagcggccgg tcgaggcgag ccgcgacggt
     1801 cggcgagata tagacgagcc gtccgttgcg atcggtttcc cagaaccagc cgcgccccgc
     1861 gcgctcgaaa tcgcgcatca gattgagcgc gcgctgctgg tcgccgatcg cgaggcgcgc
     1921 cgcgcgcgtc gcgcgccgct gatggcggac atcctcgcgc atcatcgcga tgagcaggcc
     1981 gaggaagatg aaaccggcaa cgccgaacgg ccatgacgcg accccgatcg cgccgccgag
     2041 cacggttgcc ccggcaaagg tgaagaaggc cggccgcacg atcgccgccg ccgacgccgc
     2101 gaccagcagc gacccgacat ggatcgcggt gaaggcgtcg aaatagctgc cctcgtcggg
     2161 aaccgcgatc gccgcgaaca tcgccgctgc gttgagcaag gtgccgagcg cgatcagcgc
     2221 catgcacaat atcgccatcg tccgcacgcg ctgatgcgcg atcggccgcg gccggcgcat
     2281 cgcctgcccg atcaccatca gcgcgacatc gcacggcagc ccggctgcca tcaccagcca
     2341 gtgcgagaga tggtcgagga agcccgcggc acccggcagg aaggcaaagc tcaggaaagc
     2401 aaggacgcgg ccgatcagca cataatgcca cagcttcgcg acgcgcatga cggcggtgac
     2461 gaactcgggc gaatcgaaca gcgattcgcc cgtcgtcgga tcgcccgctc ccggtccgat
     2521 gatggcgcgc cttatcccca atttcccctc gttccccgct tttgtgatcg cggttttcac
     2581 tggctggaag gggtaaccaa atccttaccg cgacgcgctg atttccgccg atattgtgcc
     2641 aaaacggaag gggttacgtt tcggttcgca acttgttata gatgggtcat gcccgccgtc
     2701 cgcctctttc cgcccgaccg ctttttcgac cgcgccgaat ggtcggcgat cacgcgcgcc
     2761 tcgtcgtggc gcggcatctg gctggtggcg cacgcgtgga tcgtcagcat cgcgctcgtc
     2821 ggtctcgccg cctggtcgca gaaccccgtc gcatggctgc tcgcggtgat cttcgtcggc
     2881 gggcgccagc tcgggctcgc gatcctgatg cacgatgcgg cgcacggtgc gctccatccg
     2941 aaccgcaaaa tcaacaattt cctcggccaa tggctgaccg gcgccgcggt cggatcggac
     3001 ctgatcgcct atcgcaccta tcacctccag catcacaaat tcacccagca gcccgaagac
     3061 cccgaccttg tgctgtcaaa gcccttcccc acgacgcgcg ccagcctgcg ccgcaagatg
     3121 ctgcgcgacc tgaccgggca gaccttcttc aaacagcgca tggcgcaatt caccttcgcc
     3181 ttcgccggcc tcaaggcgat gctgcgcggc gaggcggtgc agaaaggcgg cgcgagcacc
     3241 aaggccggaa cgccgttcaa caagcaatcc gacgacggca tgacgtcacc gacgatcgac
     3301 atcgcgggcg cgatggcggt gacgcgcgcg gtcggccgtt tcctgatcgt ccaggcggtg
     3361 ctgctcgccc tgtcgctcgc gctctatggc tggacgccct acctgctctg gctcgccggg
     3421 ctcgcgacga ccttccagct ttacctgcgc atccgcaaca tcgccgaaca tgcctgcacg
     3481 acgacgggca gcgacgatcc cttcacccac gcgcgcacca ccgacgcggg ctggctcgcg
     3541 cgcgcgacgg ttgctccata ttgggtgaat tatcacgccg aacatcatct gttcatgggc
     3601 gtgccctgct accgcctgcc agaggtccac gccgcgctcg gccgcgcggg caagcatgat
     3661 atgatgacga tcgcgcccaa ttacgccgcg gtgctgcggc gcgtcacggc caagggctag
     3721 ctcccttccc ccttgatggg ggaaggatac gcagccttgc cgcgaagcgg ttaggcggag
     3781 ttggatgggg gtgaggccgc gaccttacgc cacgcatcga cggcaccccc accactgcga
     3841 ctagcggaca agtccgcaag tctcgctgcc tcccccatca agggggaggt taaacaatgg
     3901 gttactcctc gacgaagcgc gcgccggcgc gatccttgac cgtcttttcg gggtcgaaga
     3961 tcatcccgtt catcgcgaca tagacgccgg gcggcagcgt ctgcaccgcc gcgagcgcaa
     4021 atccgacatt gaactcggcg tcgctcgcgc gcaccgacgc gggctgcatc gcgccggtca
     4081 tcacgatcgt cttgccttca atccccgcga gcacgcgccc ggtaacgacc atcgtgtcgg
     4141 tgccgtgcgt cacgaggata cgcgacgcat cgctcgcggc gaccgcggcg cggatcgcct
     4201 cacggtcggc atcgcccaat tcgaggctgt ccttgcgcat cagctgcgtg acgcggtgcg
     4261 cgacatggac attattctcg cgcagcatgt cggggatcgc cgcggggccg atctggaact
     4321 cgctcaatgc gtcgaaatag accttgtcga tcgtcccgcc ggtcgtgaaa atgtcgatga
     4381 catcactcat gccagcgcct cggcgatcaa tcgccgcgtg ttgtcgatgc cgaacaaggc
     4441 gatgaagctg cccatgcgcg gccccgcgct cgacccgagc aaggtctcgt agagcgcctt
     4501 gaaccaatcg cgcagatttt caaagccata ggcctcgttc ttgccgatct cgtacacgat
     4561 attctggata tcgtcggccg aagcctcggc gggcagcgcc gcgagcttgg catcgagttc
     4621 cttcaaggcc gcgccctcac cgcccaccgg cttgcggcgc ttcagcgtcg gcgcgacgaa
     4681 atcgcggtta tatgccatcg cattgtcgat cagccggtcg agttcgggat agctcgtcgc
     4741 atcggcgccc gcgacatatt ggccgaggta gcgccagatt tgcgccttcg acgcatcggc
     4801 gcccatcacg ccgacaaggt tgagcagcag cccgaacgtc accggcagtt cggcccgcgg
     4861 cacatcctgc ccgcgcgcga catggacatg atgcaccgga ttgccgagct tcttgtcggg
     4921 ctcctgcgcc ggataattgc cgcgcatctg gagatattcg tcgaccgcct tcggcaccac
     4981 gccgatatgg agctgcttcg ccgccttggg ctcgcggaac gcgaaaaagg cgaggctctc
     5041 ctcgctgcca tagctcagcc attcctcgat cgacaggcca ttgcccttcg acttcgaaat
     5101 cttctcgccc ttctcgtcga gaaacatctc atagatcagc ccctcgggct tgcgcccgcc
     5161 gagcgccttg gcgatcttgc ccgactggac gccgctgtcg gtcagatcct tgccgtacat
     5221 ctcgtaatcg acgccgagcg cgacccagcg catcgcccag tcgaccttcc actgcagctt
     5281 cgcgccgccg ccgaggacgc tgtgcgtgac cgtctcgccc tcatcctcga acgacacgag
     5341 ccccgcgtcg gcgtcgacga ccgtcaccgg cacctgcagc acgatccccg acttcgggct
     5401 gaccggcagg atcggcgaat aggtcgccgc gcgctcggcg cgcagcgtcg gcagcatgat
     5461 gtcgaggatg tcctgattgt ggcgcagcac attcttcagc gccgcgtcga acgcccccgc
     5521 cttatactgc tcggtcgagc tcacaaattc atattcgaag ccgaaccgat cgagaaactc
     5581 gcgcagcatc gcattgttat gatgcgcgaa actttcgaac ttgccgaacg gatcggggat
     5641 cgcggtcagc ggcttgccca gatattcgcg cagcatgtcg ccgttgggca cattgtcggg
     5701 aaccttgcgc agcccgtcca tgtcgtcgct gaacgcgacg agccgcgtcg gcgcaccgcc
     5761 ggtcagcgcc tcataggcgc ggcgcaccat cgtcgtgcgc agcacttcgt tgaaggtgcc
     5821 gatatgcggc agccccgacg gaccatagcc cgtctcgaac agcacgggct ctccaccctg
     5881 cttgccgtcc ggatagcgtt tgaccagctt gcgcgctgcc tcgaacggcc aggccttgga
     5941 cgtttgcgcg ggctcgcgca gcgaaggatg aagatgcaag tcggtcatgc cgcgcccata
     6001 gcggcaagcg gggccaaatc gaaaggcaaa attgtcccgc cgcggctcaa gatcgttacc
     6061 ggaaaattta ccatgaaggc ataatgtccc ggccatggac aattttcgcc ttcccgcgcc
     6121 ccgcggccac aagcgcaccc cggtgtcgat cgacgtcacc gtcaacggca tcctcaactt
     6181 cgtcgacggc cggatcgccg acctctccga aggcggcgcg cgcatcgatg gcgcgagcat
     6241 gcccgcgcgt tcgcgctgcg aaatccatta tggcggacag gtcacctacg cggtcgtaat
     6301 gtggtcggag catgaccgca tgggcgtgcg tttcccctac gagctcgctc agggcgcgct
     6361 ctacaacgcg ctccagagcg cccggaacgc ggcgatgatc gaacccgcgc agattttcca
     6421 cagcaaccgc ccagcggtgt tcggacgccg cgggctttcc tgaccacttc gtcgcggcaa
     6481 ccttgccgcg ttcgcctttt gtccctagcg tggaacgatg gcgcacgacc cgctcaccct
     6541 gcccgcgatc catgcgagcc acatcggcat ctggatcgcc gacacgcacg gccgcgtcca
     6601 gcgcgtcggg cgcggcgaag cgatcgcggc ggtcgcggcg accccgcatc ttctcctcaa
     6661 cgcgacgctg acc
//
LOCUS       DATMSU010000359         3023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_100972,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000359 DATMSU010000000
VERSION     DATMSU010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100972"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VN034_09650"
     CDS             complement(<1..472)
                     /locus_tag="VN034_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60908.1"
                     /translation="MPFQPRVAILVPAPDYEEKWQPAFARKAAALTAAGLVVEQRVWT
                     DPGDLAGYDLVLPLFAWGYQRDVATWYALLDRLEGEGLPVVNPVPVLRWNSDKAYLAE
                     LGGKGVAVVPTIEVAALDDARLAEARAELGVDEVVIKPAISGGADGTHRIAPGAP"
     gene            complement(628..>3023)
                     /locus_tag="VN034_09655"
     CDS             complement(628..>3023)
                     /locus_tag="VN034_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HWV60909.1"
                     /translation="AGPVARGGLRWSDRRDDFRTEILGLMKAQRVKNAVIVPTGAKGG
                     FYPKALPDVSLDRDAWFAEGTECYRIFIRSLLSITDNLVGGKVVHPKGVAILDGDDPY
                     FVVAADKGTATFSDVANALAMERDFWLGDAFASGGSKGYDHKAMGITAKGAWLSVQRH
                     FAEMGVDVQTDTIRVAGCGDMSGDVFGNGMLLSKAIQLTAAFDHRHIFLDPDPDPAKS
                     WKERDRMFNLPRSSWEDYDKKLISKGGGIFPRSQKSIPLSPEVQALLGLSVSEIDPSS
                     LISAILKAPVDLLWFGGIGTYVKAASQNNAEVGDPANDALRVDAEDLRVKAVGEGANL
                     GVTQAARIAFSAKGGRINTDFIDNSAGVDCSDNEVNIKIALNREMAEGRLSQDDRDAL
                     LVRMTDNVSDLVLEDNRLQALALSIAEKGGSAAVPSLVRIMETFEGAGRLDRKVEGLA
                     ANDELLRRAGEGRGLTRPELAVLLSTAKLALQDAIENSDLPDDPALASDLAAAFPDEM
                     QRDFAQAIADHQLRREIIATKIANRIINRMGIVHPFELVEEEGCALGDIAAAFVAVER
                     LLEMPAIWEALDEAKIDEGVRLSLFAQASSAMTSQMADLLRVTQSLSQPGPVVARLEP
                     GVDRLTAGVDALLSPSVKRQWDMLAQQLLDAGAPETLTAAVVRLFKTDGAIGIVDLAE
                     RRGDDEVAVTRAFTHLGEALGLDWAQTLAAHMSPADPWERLLVNSLARDFQQMRLAFL
                     GQLPKGDLDAAVTKWLADHAPRVEQFRSTVDRARMIPNPNGAMLSHIAGQARGLLAR"
BASE COUNT          461 a         1026 c         1041 g          495 t
ORIGIN      
        1 ccggtgcgcc gggcgcgatg cggtgcgtgc cgtcggcgcc gcccgagatc gcgggcttga
       61 tcacgacttc gtcgaccccg agctcggccc tcgcttcggc gaggcgcgcg tcgtcgagtg
      121 cagcgacctc gattgtcggc acgaccgcga cgcccttgcc gccgagctcg gcgagatagg
      181 ccttgtcgct gttccagcgc aacacgggga ccgggttgac caccggcagg ccttcgccct
      241 cgagccggtc gagcagcgca taccaggtcg cgacatcgcg ctgatacccc caggcgaaga
      301 ggggaaggac gaggtcgtag cccgcaagat cgcccggatc ggtccacacg cgctgctcga
      361 cgaccagccc ggccgcggtc agcgccgccg ccttgcgcgc gaaggcgggc tgccactttt
      421 cttcataatc gggcgccgga acgaggatcg cgacgcgggg ctggaagggc atgggggagc
      481 tttcctaaag cccctcccct tcaggggagg ggttggggtg gggctcgcgg gatagtgcga
      541 ggccaggcag cccaccccgc tgcgactagg tcggctccgc cgaaccaagt ctcgctgccc
      601 ctcccctgaa ggggaggggt ttgattgtta ccgcgccagc aatccgcgcg cctgcccggc
      661 gatatgcgag agcatcgcgc cattggggtt cgggatcatc cgcgcgcggt cgacggtgct
      721 gcggaactgc tcgacgcgcg gggcatggtc ggcgagccat ttggtcaccg ccgcgtcgag
      781 atcgcccttc ggcaactggc caaggaaggc cagccgcatc tgctggaaat cgcgcgcaag
      841 gctgttgacg agcaggcgtt cccacggatc ggcggggctc atatgcgccg ccagcgtctg
      901 cgcccagtcg agcccgagcg cttcgcccag atgggtgaag gcgcgcgtca ccgcgacctc
      961 gtcgtcgccg cgccgttcgg cgaggtcgac gatgccgatc gcgccgtcgg tcttgaacag
     1021 gcgaacgacc gcggcggtca gcgtttcggg cgcgccggcg tcgagcaatt gctgcgcgag
     1081 catgtcccac tggcgcttga ccgacgggct gagcagcgcg tcgacacccg cggtcaggcg
     1141 atcgacgccc ggctcgagcc gcgcgacgac cgggcccggc tgcgacaggc tctgggtgac
     1201 gcgcaagaga tcggccatct gtgaggtcat cgccgacgac gcttgcgcga agagcgacag
     1261 gcggacgccc tcgtcgatct tcgcttcgtc gagcgcctcc cagatcgccg gcatctcgag
     1321 caggcgctcg accgcgacga acgccgcggc gatgtcgccg agcgcgcagc cttcttcctc
     1381 gaccagctcg aacggatgga cgatgcccat gcggttgatg atgcggttcg cgatcttggt
     1441 cgcgatgatt tcgcggcgca gctggtgatc ggcgatcgcc tgcgcgaagt cgcgctgcat
     1501 ctcgtcaggg aaggccgcgg cgaggtcgct cgcgagcgcc ggatcgtcgg gcaggtcgct
     1561 gttctcgatc gcgtcctgca atgcgagctt ggcggtcgag agcagcaccg cgagctcggg
     1621 gcgcgtcagc ccgcgccctt cgcccgcgcg gcgcagcagt tcgtcgttcg cggcgagccc
     1681 ctcgaccttg cggtcgagcc ggccggcgcc ctcgaacgtt tccatgatcc gcacgagcga
     1741 cggcaccgcg gccgagccgc ccttttcggc gatcgacaac gcgagcgcct ggagccggtt
     1801 atcctcgagc accaggtccg acacattgtc ggtcatccgc acgagcagcg cgtcgcggtc
     1861 gtcttggctg agacgccctt cggccatctc gcgattgagc gcgatcttga tattgacctc
     1921 attatccgag cagtcgacgc ccgcgctgtt gtcgatgaag tcggtgttga tccgcccgcc
     1981 ctttgccgag aaggcgatgc gcgcggcctg cgtgacgccg aggttcgcgc cctcgccgac
     2041 cgccttgacg cgcagatcct cggcatcgac gcgcagcgcg tcgttcgcgg gatcgccgac
     2101 ctcggcatta ttctggctcg cggccttcac ataggtgccg ataccgccga accacagcag
     2161 gtcgaccggc gccttgagga tcgccgagat cagcgagctg gggtcgattt cgctgaccga
     2221 gaggccgagc agcgcctgca cctcggggct cagcggaatg ctcttctgac tgcgcgggaa
     2281 gatgccgccg cccttcgaga tcagcttctt gtcataatcc tcccagctcg agcgcggcag
     2341 gttgaacatc cggtcgcgtt ccttccagct tttcgctgga tcgggatcgg ggtcgaggaa
     2401 gatatggcgg tggtcgaacg cggcggtgag ctggatcgcc ttcgacagca gcatgccgtt
     2461 gccgaacacg tcgcccgaca tgtcgccgca gccggcgacg cggatcgtgt cggtctgcac
     2521 gtcgacgccc atttcggcga agtggcgctg gaccgacagc cacgcgccct tggcggtgat
     2581 gcccatcgcc ttgtggtcat agcccttcga cccgccgctc gcaaaggcgt cgccgagcca
     2641 gaagtcgcgc tccatcgcga gcgcattggc gacgtcggag aaggtcgcgg tgcccttgtc
     2701 ggcggcgacg acaaagtaag gatcatcgcc gtcgaggatc gccacgccct tcggatgcac
     2761 gaccttgccg ccgaccagat tgtcggtgat cgacaagagc gaacggatga agatgcgata
     2821 gcattcggtc ccctcggcga accacgcgtc gcggtcgagc gacacgtcgg gcagcgcctt
     2881 cggatagaag ccgcccttcg cgccggtcgg cacgatgacg gcgttcttga cgcgctgcgc
     2941 tttcatcagc ccgaggattt cggtgcggaa gtcgtcgcgg cggtcggacc agcggagccc
     3001 gccacgcgcg accgggccgg cgc
//
LOCUS       DATMSU010000360         1802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_53539, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000360 DATMSU010000000
VERSION     DATMSU010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53539"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..1283
                     /locus_tag="VN034_09660"
     CDS             531..1283
                     /locus_tag="VN034_09660"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWV60910.1"
                     /translation="MRTVRQVAKAAGISVRTLHHYDAVGLLKPGHVGANGYRWYGKEE
                     LLRLQQILFYRELGLPLAEIAAVLDDPAFDPLAALREHRAALEGRIGHYRTLIRTIDR
                     TIASLEKDEDMEDNDYYAGIAPETRDRWQREAEEFWGKDAVAAAHAKARTFSKEQVAA
                     IQAEMEAIRADFQRLFREGADPASDAVQAVTGQHYRWVSHSWTPDAAAFKRLGRHYVE
                     NPEFRGTYDDGELPGCPEFIAEAMAVYADRSL"
     gene            complement(1262..>1802)
                     /locus_tag="VN034_09665"
     CDS             complement(1262..>1802)
                     /locus_tag="VN034_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HWV60911.1"
                     /translation="RDGAVEAPTAALHFTPALLDALATAGIATETLTLHVGAGTFLPV
                     KADDTDDHVMHAEWGRIDDDTAARLNAVRAKGGRVIAVGTTSLRLLESAATDDGTIAP
                     FAGDTAIFITPGYRFKAIDGLMTNFHLPRSTLFMLVSALMGLETMQAAYAHAIEAEYR
                     FYSYGDASLLLPRQSDRSA"
BASE COUNT          350 a          578 c          570 g          304 t
ORIGIN      
        1 ccaagagcga tcgcgatcat agtaattacg ccttattaca gtagcttagc atgaaccgct
       61 tcgagtgtca tcacgtcggt cttcgacgcg aatatgcggc ggccgcctct ttgacccttg
      121 gtcgccggaa atggccgatc agccccgagt gtatcgaaat cgaacagtgg ggacgagctt
      181 ccttattacc tcgcatgcaa ctaagaaaat aaataaaagt cctatttaca tatatttaca
      241 cgacagaagc ggacaagacg ccacgatgcg gtgcgtggcg attccggggc cctccgccgt
      301 tgcgagcgga tgaaaggaca tatcgctgcg gggcaactcg acatccgctg atcgtttcac
      361 agagccgata tacaaaataa actaagtcga gcgtgctgcc gctccccgca gcgcggttcg
      421 cctttggcaa tgggtaggcc ggcgggagac aaggcatcgc aagcccgaaa gcgcttgacc
      481 ctcacgctgc gtcatccccg ataggccgtc cggtgatgag gagcagagcg atgcgaacgg
      541 tgcgacaggt cgcgaaggct gcggggatca gcgtccggac gctgcatcat tatgatgcgg
      601 tcgggctgct gaagcccggc catgtcggcg ccaacggcta tcgctggtac ggaaaagagg
      661 aactgctgcg gctgcagcaa atcctcttct accgcgagct tggcctgccg ctggcggaga
      721 tcgccgccgt gctcgacgat ccggccttcg acccgctggc cgcgctgcgc gagcatcgcg
      781 ccgcgctcga aggccggatc ggccattacc ggaccctcat ccgcaccatc gatcgcacga
      841 tcgcatcatt ggaaaaggac gaggatatgg aagacaacga ctattacgcc ggcatcgctc
      901 ccgaaacgcg cgaccgctgg cagcgcgagg ctgaggaatt ttggggcaag gacgccgtcg
      961 cggccgccca cgcaaaggca cggaccttca gcaaggaaca ggtcgccgcg atccaggccg
     1021 agatggaagc gatccgcgcc gacttccagc gcctgttccg cgaaggcgcc gatcccgcgt
     1081 ccgacgcggt gcaggcggtg accggccagc actatcgctg ggtctcgcac agctggactc
     1141 ccgacgccgc ggcgttcaag cgcctcggcc gccattatgt cgagaacccc gagttccgcg
     1201 gcacctatga cgacggtgaa ctaccgggat gccccgaatt catcgccgaa gcgatggcgg
     1261 tctacgccga ccggtcgctc tgacgcggca gcagcaggct ggcgtcgcca tagctgtaga
     1321 agcgatactc ggcttcgatg gcatgggcgt aggccgcctg catcgtctct agccccatca
     1381 gcgcgctgac cagcatgaac aaggtcgagc gcggcaggtg gaaattggtc atcagcccgt
     1441 cgatcgcctt gaagcgatag ccgggcgtga tgaagatcgc ggtgtcgccc gcgaacggcg
     1501 cgatcgtacc gtcgtcggtg gcggcgcttt cgagcaggcg caggctggtc gtgccgaccg
     1561 cgatcacccg gccgcccttc gcacgcaccg cgttcagccg cgccgcggta tcgtcgtcga
     1621 tccgtcccca ttcggcgtgc atgacatggt cgtcggtgtc gtctgccttg accggcagga
     1681 aggtgcccgc gccgacgtgc aaggtcagcg tctcggtcgc gattcccgcc gtcgccagcg
     1741 catccagcag cgcgggcgtg aagtgcagcg ccgcggtcgg cgcctcgacc gcgccgtcac
     1801 gc
//
LOCUS       DATMSU010000361         3171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_6694, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000361 DATMSU010000000
VERSION     DATMSU010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6694"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2220)
                     /locus_tag="VN034_09670"
     CDS             complement(<1..2220)
                     /locus_tag="VN034_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60912.1"
                     /translation="MGTRSQVFMTATASAIALSASPAIAQDNGDEIIVTGSRIAKSEF
                     TSADPIQIIDPETAKLQGQVQLADVLQQVPAAQGSIQITSAISNRFVANGGNDVQTVS
                     LRGLGAERTLVLINGRRAGPAGIRGSVAPFDLNVLPLSVVRQVEILKTGASSIYGSDA
                     VAGVVNILTRDDLDGFEAGGFSSITEHGGGESYGADASFGKIFDRGHFFATVDIFRQQ
                     NLTRRDRGFLFCSEEYLKREADGSRADIVDFRTGRPACSSTQANMIVFSDFSGVDENG
                     FPTFAPGLIAPNGQAIFAGQYGAEFAGVGIPINAYNPIGVFGPADFFGVNFDGPSTGA
                     LNQFEPAERDMDVFSGVRRVSAFAEGAYDVTGGITAYGEFLFSNRKSHNNGVQRIELQ
                     QFTGASMLPFFLCDPTAYNCDPGDMGDPLNTEFGGNLILRPRVLVKSESEADVDYYRG
                     VLGLRGDFGAGWKWDVHGQHSRSDANYTQDVIYQDALASQTLRTRSCAGTVTAIRGVP
                     CIDIDFTDPRVLRGDFTPEERAFLTGRETGRTLFKQSSAEALVTGKLFALPAGPVGVA
                     FGANIRRDEIDDTPGEATRAGNVANFTTSGITAGRTVSKEAFGEVELPLLAGVPMIER
                     LTLSGAARYTHVEATSRGAQDSFSDTTWKVGADWAVTDWLRFRGTWGTSFRAPALFEL
                     FLQDQTGFLGQPDIDPCIQSAARLAAGAISQRIFDNCAAAGIAPDYAGGIAPATITSG
                     "
     gene            2397..2717
                     /locus_tag="VN034_09675"
     CDS             2397..2717
                     /locus_tag="VN034_09675"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HWV60913.1"
                     /translation="MGEALDRLESIVRDRLAAGDAEASYVASLAAKGRGKIAQKLGEE
                     AVETVIAALTEDDAALTGEAADLVFHLTILLADRGLSWNDIATELERRHGTSGHAEKA
                     GRPQ"
     gene            2729..3106
                     /locus_tag="VN034_09680"
     CDS             2729..3106
                     /locus_tag="VN034_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWV60914.1"
                     /translation="MPIDATLPYDDSNIFARILRGELPSKTVYEDEFALAFHDINPQA
                     PLHILVIPKGAYVSWDDFSERGSDAEIAGFVRAVGKVARDQGLVAPGYRLLANTGLDS
                     HQEIPHLHVHIFAGQPLGPMLAK"
BASE COUNT          545 a         1102 c         1014 g          510 t
ORIGIN      
        1 gcccgaggtg atcgtcgccg gggcgatgcc gccggcataa tccggcgcga tgcccgccgc
       61 ggcgcaattg tcgaagatgc gctggctgat cgcgcccgcc gcgagccgcg ccgcgctctg
      121 gatgcacgga tcgatgtccg gctggccgag gaagcccgtc tggtcctgaa ggaacagctc
      181 gaacagcgcc ggggcgcgga acgaggtgcc ccaggtgccg cggaagcgca gccagtcggt
      241 caccgcccag tcggcgccga ccttccacgt cgtatcgctg aagctgtcct gcgcgccgcg
      301 cgacgtcgcc tcgacatgcg tatagcgcgc cgcgcccgac agcgtcagcc gctcgatcat
      361 cggcacgcct gcgagcaaag gcagctcgac ctcgccaaag gcctccttcg atacggtgcg
      421 ccccgcggtg attcccgagg tcgtgaagtt ggcgacattg cccgcgcgcg tcgcctcgcc
      481 cggcgtgtcg tcgatctcat cgcgacggat gttcgcaccg aaggcgacgc cgaccggtcc
      541 ggcgggcagc gcgaacagct tgcccgtcac cagcgcctcg gccgagctct gcttgaacaa
      601 ggttcgcccc gtctcgcggc ctgtcaggaa ggcgcgctcc tccggcgtga aatcgccgcg
      661 cagcacgcgc gggtcggtga agtcgatgtc gatgcatggc acgccgcgga tcgcggtcac
      721 cgtccccgcg cacgagcgcg tgcgcagcgt ctgcgacgcg agcgcatcct gatagatgac
      781 gtcctgtgta tagttcgcgt cggagcggct gtgctggccg tggacgtccc atttccaccc
      841 cgccccgaag tcgccgcgca gtcccagcac accgcgataa taatcgacgt cggcctcgct
      901 ttcggatttc accagcacgc gcgggcgcag gatcaaattg ccgccgaact cggtgttgag
      961 cggatcgccc atgtcgcccg ggtcgcaatt atacgccgtc ggatcgcaga ggaaaaaggg
     1021 cagcatcgac gcgcccgtga actgctgaag ctcgatccgc tggacgccat tgttgtgcga
     1081 cttgcggttg ctgaacagaa actcgccata agcggtgatg ccgcccgtca catcataagc
     1141 cccctcggca aaggcgctga cgcggcgcac gcccgagaag acgtccatat cccgctcggc
     1201 gggctcgaac tggttgagcg cgccggtcga cgggccgtcg aaattgaccc cgaagaaatc
     1261 ggccgggccg aacacgccga tcggattata ggcattgatc gggatgccga cgcccgcgaa
     1321 ctcggcgcca tattggcccg cgaagatcgc ctgcccgttc ggcgcgatca gcccgggggc
     1381 gaaggtcggg aaaccatttt cgtcgacccc cgaaaaatcg ctgaacacga tcatattcgc
     1441 ctgcgtgctg ctgcaagccg ggcgtcccgt gcggaaatcg acgatgtcgg cgcggctgcc
     1501 gtcggcctcg cgcttgagat attcttccga acagaacagg aagccgcggt cgcggcgcgt
     1561 cagattctgc tggcggaaaa tatcgaccgt cgcgaaaaaa tgcccgcggt cgaatatctt
     1621 gccgaagctc gcgtcggcgc catagctctc gccgccgcca tgttcggtga tcgacgaaaa
     1681 gccgcccgcc tcgaagccgt cgaggtcgtc gcgggtcaat atattgacca cgcctgctac
     1741 ggcatcggag ccatagatcg acgaggcgcc ggtcttgagt atctcgacct ggcgcaccac
     1801 cgacagcggc agcacattga ggtcgaaggg cgcgaccgat ccgcggatgc ccgcgggacc
     1861 cgcgcggcgg ccgttgatca gcaccagcgt ccgctcggcg ccgaggccgc gcagcgacac
     1921 cgtctgcacg tcgttgccgc cattggcaac gaagcggttc gagatcgccg aagtgatctg
     1981 gatcgacccc tgcgccgccg gcacctgctg cagcacgtcg gcgagctgga cctgcccctg
     2041 cagcttcgcc gtctcggggt cgatgatctg gatcgggtcg gcgctggtga attcgctctt
     2101 cgcgatgcgc gagccggtga cgatgatttc gtcgccattg tcctgcgcta tcgccgggct
     2161 agccgacaga gcgatcgccg aggccgttgc ggtcataaac acctgggagc gggtgcccat
     2221 tttaatatcc ttgtccgaat agaagttgat ctcgcccccc ggcgcgcccg aaattagccg
     2281 cagggcgttt cgatatggtt tacgcggcgg taatttttcg ggcgtcgccc ccgcttgccg
     2341 cgaccggcgc gcgccgctac gcacgcgctc gaaaggatcg aatgatggtg agcgcgatgg
     2401 gcgaagcatt ggaccggctg gaatcgatcg tccgcgaccg tctcgcggcg ggcgacgccg
     2461 aggcttccta tgtcgcgagc ctcgccgcca agggccgcgg caagatcgcg caaaagctcg
     2521 gcgaggaagc ggtcgagacg gtgatcgcgg cgctcaccga ggacgacgcc gcgctgaccg
     2581 gcgaggcggc cgatctcgtc ttccacctca ccatcctgct tgccgaccgc ggcctgtcgt
     2641 ggaacgatat cgccaccgaa ctggagcgcc gccacggcac ctcgggccac gccgaaaagg
     2701 ccggccgtcc ccaataggag ctttccccat gccgatcgac gcgacccttc cttatgacga
     2761 cagcaacatc ttcgcgcgca tcctgcgcgg cgagctgccg tcgaagacgg tctacgagga
     2821 cgaattcgcc ctcgccttcc acgacatcaa cccgcaggcg ccgctgcaca tcctcgtcat
     2881 ccccaagggc gcttacgtga gctgggacga tttttcggag cgcggcagcg acgccgagat
     2941 cgcgggtttc gtccgcgcgg tcggcaaggt cgcgcgcgat cagggcctcg tcgcccccgg
     3001 ctaccgcctg ctcgccaaca ccggcctcga cagccatcag gaaatccccc acctccacgt
     3061 ccacatcttc gccggccagc cgctcggccc gatgctcgca aaatagcacg tcgcccccgc
     3121 gaaggcgggg gccgctagca accttgcgct atgccgacgg cggcccccgc c
//
LOCUS       DATMSU010000362         8080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_147900,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000362 DATMSU010000000
VERSION     DATMSU010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147900"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VN034_09685"
     CDS             complement(<1..521)
                     /locus_tag="VN034_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011907020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HWV60915.1"
                     /translation="MIDERAECWTEMPGLIAAVQAAGDAIGLPYVAAQADLGDPEPMS
                     DEAGRPYAETSFRWVDPDYAYWRDRKLALHIAFLTAARLVGEPFYYSDGRLRTWRPTQ
                     LLDEVDCSQAKNTPNFGEAIIAPVHLPRGLVGAVFWCSREPVGVEAIFAEQAGHLHNL
                     AVKLVATHNEARGR"
     gene            669..3107
                     /locus_tag="VN034_09690"
     CDS             669..3107
                     /locus_tag="VN034_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60916.1"
                     /translation="MRIFLAAGTAMGLVAGAPAFAAEETAPDSVAAEGDIIVTAQRRA
                     ESMNDVGMAIQAVDGETLENLRVTDMRDLTAVAPSFTVSQSYQGVPTYTLRGIGFNTI
                     NLSSTSTVGTYVDEVAYAYPIMNTGPVMDIERVEVLKGPQGTLYGRNTTAGLINFITA
                     KPTDSFEGSIRADLGNYQTYNIGGHISGPLGEGVAARIAFRSENSDKGWQVSNTRGER
                     LGEVDKLGVRGSLAFDPAPGTHFDLSVTWWRNRSDTVAGQGIGFTPATNPVSGTSNSR
                     FFNAPGLADYLAANFPTKASQADWASEASRSADIGTGLGLDGPLAEDNRFWGLKLRWD
                     QDFGETMKLVSLTSYNDFKRNALSDWSGAPFEILLQNTVGRIKSFAQELHVEGEAGAI
                     NWLVGAYYANDRIIDSNRTMLGQNANVGLIRGAGVPLLGTPFNSGGYTPLELSQAFRT
                     YEDFGRIRTKTWSIFGNADAELTEQLKLTVGVRYTEDKQRYNGCSRDFNGNMLPNVNV
                     VNRALYFQTYGVLAPEIAEGQCNTFDPETGTFGEVQSLLDENNVAWRAALDWSPNDDT
                     LLYASVSRGYKAGTTPINAANLARQNAPVTQEKLTAYEIGVKATLADRLVQANLSAFY
                     YDYRDKQISTYFADPIYTALSRLDNVPDSEAYGVEGELTIRPATGFTIAANALWLKTR
                     INDYDGTNAAGQPENFDGAEFIYSPEFQGSVTIAYDTPVSDTLNLTGAVSARYQSKSN
                     TIFEDLDLYKVDAYGTVNASLGLKSESGWSVSLWAKNLFDKYYWSAVASNANVVVRFA
                     NQPRTWGLTVGYDF"
     gene            3123..4034
                     /locus_tag="VN034_09695"
     CDS             3123..4034
                     /locus_tag="VN034_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HWV60917.1"
                     /translation="MVRAPMQNDYLGTAIDFAAPLGEPALLPPDSVQWRVYKNPIALG
                     IGGIAAVLLEFAEPRIRSGVWDHSTYKTDPIGRSRRTGLVAMLACYGPASAAKEVIGK
                     VNRMHGKVKGETPAGDRYRAMDPVLLDWVAATASYGFLMAYDRFVRPVSDADKDRFMA
                     DGDAIGSEFGARRTPPTVAAFMEMMAELEPRFEPHPIIFEFLDIIQSGKAAPGVPRFL
                     HRAIARASVSLLPDHIRRKLELGPRYDLTRLDRSALRIAGRLADRHVDRAAPHCQASV
                     RLGLPHDFLFKSPAEQRRLLAGMRLAA"
     gene            complement(4031..5728)
                     /locus_tag="VN034_09700"
     CDS             complement(4031..5728)
                     /locus_tag="VN034_09700"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HWV60918.1"
                     /translation="MGSPRRFAPSLLLLCSSMLVSASTAAQAPAYDLIIRGGTLYDGS
                     GKPPVVGDVAIKDDRIVAVGKVDGKAKREVAAKGMAVAPGFINMLSWATESLIADPKS
                     QSDIRQGVTLEVMGEGWSMGPMNAKMKALDVERQGDIKYDIEWTSLGDYLSYLEKRGI
                     STNVASFVGAATVRVHELGEGDVDPNPEQLARMRALVRHAMNEGAMGVGSSIIYAPGS
                     YAETDELVALTSEAAKCGGMYISHMRSEGDRIEEAVDELIEISRRSGAPAEIYHLKMA
                     GRSNWGKLDPIVKKIEDARAAGLRITTDMYTYTAGATGLDAAMPTWVQAGGLEKWIER
                     LKDPAIRARVAEEMKKPGSDWENLYFGAGADKMILSGFKNDALKPLTGKTLAEVAAMR
                     GKSPEETAMDLVVEDGSRVGTVYFLMSEDNVRKQIQLPWMSFGSDAASQAPEGVFLKS
                     GAHPRTYGNFARLLGRYVRDEKLIPLEQAVYRLTTLPATNLGIKDRGALKPGYYADVV
                     VFDPAAIGDRSTFEKPHQYSVGVRDVFVNGVAVLQGGEHSGATPGRAVRGAGFNRCS"
     gene            complement(5752..6375)
                     /locus_tag="VN034_09705"
     CDS             complement(5752..6375)
                     /locus_tag="VN034_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HWV60919.1"
                     /translation="MADRATPPTLGAVMKGIRARNGWTLKEMSAKSGIPVSTLSKVEH
                     DRLTLSYDKLQQLSQRLQIRMSDLFAEDEEDAAPRVTGRRSIAAIERAARVTTDNYDY
                     HYLCTDLRQKRMIPILTRIRAHSASEFGELVHHQGEEFIYVLEGRIEVHSEFYDPVTL
                     DVGQGIYIDSSMGHAYVVADGFEEALVLGVCSSADDGLMDSLMSLHG"
     gene            6518..>8080
                     /locus_tag="VN034_09710"
     CDS             6518..>8080
                     /locus_tag="VN034_09710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV60920.1"
                     /translation="MRFIRKAMIGLAAFAALPLGAQGPVPAPAVKKAEAVVPAAKTAA
                     APAPLDPKDLEAWMDGYLPYALERGRIPGAVVVVVRGGEVVLQKGYGYSDVAKRTPVS
                     PETTLFRPGSVSKLLTWTAVMQQVEAGKIDLDKDVNAYLDFKIPPFEGKPVTMRNIMT
                     HTAGFEESVRHLISSDPKAVMTLKKQMPLALPERVFAPGTTPAYSNYATALAGYIVER
                     VSGEPFDNYIENHIFEPLGMTHSSFRQPLPARLAPHMSKGYPDITQKPEPFEMVIPAP
                     AGSLSASGADMAKFMIAHLNDGAGLMKPETAKMMHDFKAPGVGPLNSMALGFYEQWVN
                     GQRAIAHGGDTVWFHSYLWLFPDADTGVFISMNSAGKDGAAGAVRSALFHKFADRYLP
                     GPAEKPAQVDAKTAAQHAQMMAGNYISSRGSFTNFMSLFGLLGQATIGLTEDGKITLP
                     ALDGLGAGARDWVEVEPFVWRDTGTGERLAAEVKDGRVVRWSIDAGSPFMVFEPAPAS
                     VNAAWLNPALVLA"
BASE COUNT         1432 a         2652 c         2528 g         1468 t
ORIGIN      
        1 cggccgcgcg cttcgttgtg cgtcgcgacc agcttgaccg cgaggttgtg tagatggccg
       61 gcctgctcgg cgaagatcgc ctcgaccccg acgggctcgc gcgagcacca gaagaccgcg
      121 ccgaccaggc cgcgcggcaa gtgcaccggc gcgatgatcg cttcgccgaa attgggcgtg
      181 ttcttcgcct gcgagcagtc gacctcgtcg agcagctgcg tcgggcgcca cgtgcggagg
      241 cgcccgtcgc tgtagtaaaa gggttcgccg accagccgcg ccgcggtcag gaaggcgatg
      301 tgcagcgcca gcttgcggtc gcgccagtaa gcatagtcgg gatcgaccca acgaaagctc
      361 gtttcggcat agggacgacc ggcctcgtcg ctcatcggct cggggtcgcc gaggtcggcc
      421 tgcgccgcga catagggcag cccgatcgcg tcgccagccg cttgcacggc ggcgatcagc
      481 cccggcattt cggtccagca ttccgccctc tcgtcgatca cgcttttgtc cctccactcc
      541 ctgacacagg tgtcagcttg tccctgcggc cgcaattcta tgttcttgca cggtccggtt
      601 tcgcgcgaag ctcgcacaaa gacgagctcc gcgacaagct gtttgaggga ggatgggtga
      661 tgaaggcaat gcgtattttc ttggccgcgg gtaccgcgat ggggcttgtc gccggggcac
      721 cggcgttcgc ggccgaagaa accgcgcccg atagcgttgc tgccgaaggc gacatcatcg
      781 tcaccgcgca gcgccgcgcc gaatcgatga acgacgtggg catggcgatc caggcggtcg
      841 atggcgagac gctcgagaat ctgcgcgtga ccgacatgcg cgacctgacc gcggtcgcac
      901 cgagcttcac cgtttcgcaa agctatcagg gggttccgac ctatacgctg cgcggcatcg
      961 gcttcaacac gatcaacctc tcctcgacct cgaccgtcgg tacttatgtc gacgaggtcg
     1021 cctatgccta tccgatcatg aacaccggcc cggtgatgga tatcgagcgc gtcgaggtgc
     1081 tgaaagggcc gcagggcacg ctttacggcc gcaacaccac cgcgggcctg atcaacttca
     1141 tcaccgccaa gccgaccgac agcttcgagg gcagcatccg cgccgacctc ggcaattacc
     1201 agacgtataa tatcggcggc catatctcgg gcccactcgg cgagggcgtc gccgcgcgca
     1261 tcgcctttcg cagcgagaat agcgacaagg gctggcaggt cagcaacacg cggggcgaac
     1321 ggcttggcga ggtcgacaag ctcggcgtgc ggggttcgct cgcgttcgat cccgccccgg
     1381 gcacgcattt cgacctgtcg gtgacttggt ggcgcaacag gtcggatacc gttgcggggc
     1441 aggggatcgg ttttaccccc gcaaccaatc cggtcagcgg cacgagcaac tcgcgcttct
     1501 tcaacgcgcc cgggctcgcc gactatctcg cggccaattt cccgaccaag gcgagccagg
     1561 ccgactgggc gtccgaggcg tcgcgttcgg ccgatatcgg caccgggctc ggcctcgatg
     1621 gaccgctcgc cgaggacaat cgtttctggg ggctcaagct gcgctgggat caggactttg
     1681 gcgagacgat gaagctcgtc tcgctcacca gctataatga tttcaagcgc aacgcgcttt
     1741 ctgactggag cggcgcgccg ttcgaaatcc tgctccagaa cacggtggga cggatcaaga
     1801 gcttcgcgca ggaactgcat gtcgagggcg aggcgggggc gatcaactgg ctcgtaggtg
     1861 cctattatgc caacgatcgc atcatcgatt cgaaccgcac gatgctcggc cagaatgcca
     1921 atgtcgggct gatccgcggg gcgggcgtgc ccttgctagg gacgccgttc aactcgggcg
     1981 gctacacccc gctcgaactt tcgcaggctt tccgcaccta cgaggatttc ggccgtatcc
     2041 gcaccaagac gtggagcatc ttcggcaacg ccgacgccga attgaccgag cagctcaagc
     2101 tgaccgtggg agtgcgctat accgaggaca agcaacgcta caacggctgc tcgcgcgact
     2161 tcaacggcaa tatgctgccc aacgtcaacg tcgtgaaccg cgcgctctat ttccagacct
     2221 atggcgtgct cgcgcccgaa atcgccgagg ggcagtgcaa caccttcgat cccgagacgg
     2281 gcacctttgg cgaggtgcag tcgctgctcg acgaaaacaa cgtcgcgtgg cgcgccgcgc
     2341 tcgactggtc gccgaacgac gataccctgc tctatgcctc ggtatcgcgc ggctataaag
     2401 cggggacgac gccgatcaac gccgcgaacc tcgcgcgcca gaatgcgccg gtgacacagg
     2461 agaagctcac cgcctacgag atcggggtca aggcgacgct cgcggatcgt ctggtgcagg
     2521 ccaatctttc ggccttctat tacgactacc gcgacaagca gatcagcacc tatttcgccg
     2581 acccgatcta caccgcgctg tcgcgcctcg acaatgttcc cgacagcgag gcttatggtg
     2641 tcgagggcga gctgacgatc cggccggcga cgggcttcac gatcgccgcc aacgcgctgt
     2701 ggctgaagac gcgaatcaac gattatgacg gcaccaacgc cgcgggacag ccggagaatt
     2761 ttgacggcgc cgaattcata tacagccccg aatttcaggg cagcgtgaca atcgcctatg
     2821 acacgccggt cagcgatacg ctcaatctga ccggcgcggt gagcgcgcga tatcagagca
     2881 agtcgaacac gatctttgag gatctcgatc tctacaaggt cgatgcctat ggcactgtca
     2941 acgcgagcct tggcctcaaa agcgaaagcg gctggtcggt atcgctgtgg gcgaagaatc
     3001 tcttcgacaa atattattgg tcggcggtcg ccagcaacgc caatgtcgtc gtgcgcttcg
     3061 ccaatcagcc gcgaacctgg ggcctcaccg tgggttatga cttctagaag gagcgaaaga
     3121 aagtggtgcg cgcgccgatg cagaatgatt atctggggac ggcgatcgat ttcgcggcgc
     3181 ctctcggcga acccgcgctg ctgccgcccg acagcgtcca gtggcgcgtc tacaagaatc
     3241 cgatcgcgct cggcatcggc ggcatcgcgg cggtgctgct cgaattcgcc gagccgcgca
     3301 tccgctcggg ggtgtgggat cattcgacct acaagaccga cccgatcggc cgttcgcgcc
     3361 gcaccggcct cgtcgcgatg cttgcctgtt atggccctgc gagcgccgcg aaagaggtga
     3421 tcggcaaggt caaccgcatg cacggcaagg tgaagggcga aacaccggcg ggcgacagat
     3481 atcgcgcgat ggacccggtg ctgctcgact gggtcgccgc gaccgcctcc tatggcttcc
     3541 tgatggccta tgaccggttc gtccgccccg tcagcgacgc cgacaaggat cgcttcatgg
     3601 ccgatggcga tgcgatcggg agcgaattcg gcgcgcggcg cacgccgccg acggtggccg
     3661 ccttcatgga gatgatggct gaactcgaac cgcgcttcga gccgcatccg atcatcttcg
     3721 aattcctcga tattatccaa tcgggcaaag ctgcgccggg cgtgccgcgc ttcctgcacc
     3781 gcgctatcgc gcgcgcgtcg gtatcgctgc tgcccgatca tattcgccgc aagctcgaac
     3841 tcggcccgcg ctacgatttg acgcgtctcg atcgcagcgc gctacgcatc gccggccggc
     3901 tcgccgatcg ccatgtcgat cgcgccgcgc cgcattgcca ggcgagcgtc cggctcggcc
     3961 tgccgcatga tttcctgttc aaatcgcccg ccgagcagcg gcgattgctg gctgggatgc
     4021 ggctagccgc ctaggaacaa cggttgaagc ccgcgccgcg caccgcgcgt cccggcgtcg
     4081 cgccgctatg ctcgccaccc tgcagcaccg cgacgccgtt gacgaacacg tcgcgcacgc
     4141 cgaccgaata ttggtgcggc ttttcgaagg tcgagcggtc gccgatcgct gccggatcga
     4201 agacgaccac atcggcataa tagcccggct tcagcgcgcc gcgatccttg atgccgagat
     4261 tggtcgcggg cagggtggtg agccggtaca ccgcctgttc gagcgggatc aatttctcgt
     4321 cgcggacata gcggccgagc agccgcgcga aattgccgta ggtgcgcggg tgggcgcccg
     4381 attttaggaa gacgccctcg ggcgcctgcg acgccgcgtc ggacccgaaa ctcatccacg
     4441 gcagctggat ctgcttgcgc acattatctt cggacatcag gaaatagacg gtgccgacgc
     4501 gcgagccgtc ctcgacgaca agatccatcg ccgtctcttc gggcgacttg ccgcgcatcg
     4561 ccgcgacctc cgccaacgtc ttgccagtga gcggcttcag cgcgtcattc ttgaagcccg
     4621 agaggatcat cttgtcggca cccgcgccga aatagagatt ttcccagtcg ctgcctggct
     4681 tcttcatttc ctcggcgacg cgcgcgcgga tcgcggggtc tttcagtcgc tcgatccact
     4741 tctcaagccc gcccgcctgc acccaggtcg gcatcgcggc gtcgagtccg gtcgcgccgg
     4801 cggtataggt gtacatatca gtggtaatgc gcagccccgc ggcgcgggca tcctcgatct
     4861 tcttcacgat cggatcgagc ttgccccagt tgctgcgccc cgccattttc aagtgataga
     4921 tttcggcggg cgcgcccgag cggcgcgata tctcgatcag ctcgtcgacc gcctcttcga
     4981 tccggtcgcc ttcggatcgc atgtggctga tatacatgcc gccgcatttc gcggcctcgc
     5041 tcgtcagcgc gacgagttcg tcggtttcgg cgtaggaacc gggcgcatag atgatcgaac
     5101 taccgacccc catcgcgcct tcgttcatcg catgtcgcac cagcgcgcgc atccgggcga
     5161 gctgttcggg gttgggatcg acgtcgcctt cgcccagctc gtgcacgcgc accgtcgccg
     5221 cgccgacgaa gctcgcgaca ttggtcgata tgccgcgctt ctcgaggtag gagagataat
     5281 cgccgaggct cgtccactcg atgtcatatt tgatgtcgcc ctggcgctcg acatccagcg
     5341 ccttcatctt cgcattcatc ggccccatcg accagccttc gcccatcacc tccagcgtca
     5401 cgccctgccg gatgtcgctc tggctctttg ggtcggcgat cagcgattcg gtcgcccagc
     5461 tcagcatgtt gatgaagccc ggcgcgaccg ccatgccctt ggccgcgacc tcgcgcttcg
     5521 ccttgccatc gaccttgccg accgcgacga tacggtcgtc cttgatcgcg acatcgccca
     5581 cgacgggcgg cttgcccgat ccgtcataga gggtgccgcc gcggatgatc aggtcatagg
     5641 ccggcgcctg cgccgccgta gaggccgaaa ccagcatcga gctgcacagc agcaaaagcg
     5701 agggtgcgaa gcgccggggc gatcccattt ttttctcccg tttttagtat tttatccgtg
     5761 aaggctcatg agcgaatcca tcagcccgtc gtcggcgctc gaacagacgc cgaggacgag
     5821 tgcctcttcg aacccgtcgg cgacgacata ggcgtggccc atcgacgaat cgatatagat
     5881 gccttggccg acgtcgagcg tgaccggatc gtaaaattcg ctgtgcacct cgatccgccc
     5941 ttcgaggaca tagatgaatt cctcgccctg atgatggacg agctcgccga actcgctcgc
     6001 gctgtgcgcg cggatgcgtg tcaggatcgg gatcatgcgt ttctggcgca ggtcggtgca
     6061 gagataatga tagtcgtaat tgtcggtggt gacgcgcgcg gcgcgttcga tcgcggcgat
     6121 gctgcggcgg ccggtgacgc gcggcgcggc gtcctcctca tcctcggcga agaggtccga
     6181 catgcgaatc tgtagccgct ggctcagctg ctgaagcttg tcgtaactca gcgtgagccg
     6241 gtcgtgctcc accttcgaca gcgtcgatac ggggatgccc gacttcgcgc tcatttcctt
     6301 gagcgtccag ccgtttcgcg cgcgaatgcc cttcatcacc gcgccgagcg tcggcggggt
     6361 tgcgcggtcc gccatcagtt tttccattcc atgtttgaca attttctaat caggatcatt
     6421 atgtccctat cgggctaaca gttgttggtc ccttggtaag gggtcgcgcg gcttgccgca
     6481 aggtgtgaca ggcgcgccag ataataaggg gagagcgatg cgtttcatcc gcaaggcgat
     6541 gatcgggctg gcggcctttg ccgcgctgcc gctaggggcg caggggccgg tgccggcgcc
     6601 cgccgtcaaa aaagccgaag ccgtggtgcc ggccgccaaa acggccgcgg cgccggctcc
     6661 gctcgatccc aaggatcttg aagcgtggat ggacggctat ctgccttatg ccctcgaacg
     6721 cgggcgcatc ccgggcgcgg tggtcgtcgt cgtgcgcggc ggcgaggtcg tgcttcagaa
     6781 aggctatggc tattccgatg tcgccaagcg gacgccggtc tcgcccgaaa ccacgctgtt
     6841 ccgccccgga tcggtgtcga aactgctgac ctggaccgcg gtgatgcagc aggtagaggc
     6901 cggcaagatc gacctcgaca aagacgtcaa cgcctatctc gatttcaaga ttccgccctt
     6961 cgagggcaag ccggtgacga tgcgcaacat catgacgcac accgccggct tcgaggaatc
     7021 ggtgcgccac ctgatcagca gcgatccgaa ggcggtgatg acgctcaaga agcagatgcc
     7081 gctcgcgctg cccgaacgcg tgttcgcgcc gggtacgacc ccggcctatt ccaactatgc
     7141 caccgcactc gcgggctata tcgtcgagcg cgtcagcggc gagccgttcg acaattatat
     7201 cgaaaaccat atcttcgagc cgctcggcat gacgcattcg tcgttccgcc agccgcttcc
     7261 agcgcggctc gcgccgcata tgtcgaaggg ttatcccgac atcacgcaaa agcccgagcc
     7321 gttcgaaatg gtgatccccg cgcccgcggg cagcctgtcg gcgagcggtg ccgacatggc
     7381 gaagttcatg atcgcgcatc tcaatgacgg tgcggggctg atgaaacccg aaaccgcgaa
     7441 gatgatgcat gatttcaagg cgcccggcgt cggcccgctc aacagcatgg cgctcggctt
     7501 ctacgaacaa tgggtcaacg gccagcgcgc gatcgcgcac ggcggcgaca ccgtctggtt
     7561 ccactcctac ctctggctgt tccccgatgc cgacaccggc gtcttcatct cgatgaacag
     7621 cgcgggcaag gacggcgcgg cgggggcggt gcgcagcgcg ctcttccaca aattcgccga
     7681 tcgttacctc ccggggccgg ccgaaaagcc ggcacaggtc gatgccaaga ccgccgcgca
     7741 gcacgcgcaa atgatggccg gaaattatat cagcagtcgc ggctccttca ccaatttcat
     7801 gagcctgttc ggcctgctcg gccaggcgac gatcgggctg accgaggatg gcaagatcac
     7861 gctccccgcg ctcgacgggc tcggcgccgg cgcgcgcgac tgggtcgagg tcgaaccctt
     7921 tgtgtggcgc gacaccggca cgggcgaacg tcttgctgcc gaagtcaagg acggccgcgt
     7981 cgtgcgctgg agtatcgacg ccggctcgcc cttcatggtg ttcgaacccg cgcccgccag
     8041 cgtcaacgcc gcctggctca atccggcgct ggtcctcgcc
//
LOCUS       DATMSU010000363         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_53626, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000363 DATMSU010000000
VERSION     DATMSU010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53626"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..741)
                     /locus_tag="VN034_09715"
     CDS             complement(58..741)
                     /locus_tag="VN034_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HWV60921.1"
                     /translation="MLIAVPDLLSPAEVADIRAIIDAAEWVDGNATSGHQSALAKHNE
                     QLPEDGEAAKQAGRLILEALGRSPLFFASALPLKIYPPLFNRYGAGQEFDTHVDNAIR
                     LKRGSDFRIRSDLSITIFLEAPEDYDGGELLIEDHYGVQRVKLPAGHAVVYPSSSLHR
                     VTPITRGRRVASFFWLQSMVRDAEKRRTLFDLDRAVQRLTGELGGKDRSVIELTGVYH
                     NLLRLWADS"
     gene            complement(839..3145)
                     /locus_tag="VN034_09720"
     CDS             complement(839..3145)
                     /locus_tag="VN034_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60922.1"
                     /translation="MAADTGADASSEQQSERERRDEREQIIVNGQHYQTQQESPKSTR
                     PVRDTPQTVTVITGETIEQQNLLTLRDMLSNVPGITFGAAEGGSPPADSINFRGYSAG
                     GDITQDGVRDSAAYSRSDSFNLEQLEIVNGANSVQSGAGSVGGSINIVTKRPLDETRV
                     IGTAGIGTDNYYRGTVDANLRLNDLIAVRLNAMAHKNDVPGRDVDTYKRWGVAPSISI
                     GMAGPTKLTLQYLHQEDDNIPQYGVPYYDGLVPGAHRSDYFGYRNVDRQEITVDQATA
                     IFEHDFSDSWSIRNLARWQKVSQLTVVGPPQGTYCLSTGVTPTGGSCTITVSGAPFTV
                     PDGYYYIGGPRGTLRDSTNELMYDQVDLRGTFNTGAIEHTLVIGASALWEKYTLNNGN
                     ALRNADGTPFYTRYPLVNIANPNEVVVGPALPPGFTYGNNVYDGPVNFTYTGRQRGEV
                     DNYAVYLFDAMKLGKFELNGGVRYEKSSGWNRNGLGITVPREYSDNLFSYRIGLVYKP
                     VEAVTLYAAYGNSKTPSQSSVNGSCALPNDDGLNGNCNVEPESAKNYEIGVKAEVAGG
                     RLLLSAAAFRNERDSYRVNSGDPTLPDQVLDGHSRVDGIALSAVGQILPGWSITANYT
                     YLDSEILQSVSDFCLANPGEGTCTNSPAFPDPVAGARLQNTPKHSGSLFTTYEFPFGL
                     KIGYGATYQGKFTFNLPTATADPELFQSDDYWVHNAYLAYDFTDTISAQLNVKNFTDK
                     LYFTRIRNNGWATPGDARSAVLTVSVGF"
     gene            complement(3345..>3638)
                     /locus_tag="VN034_09725"
     CDS             complement(3345..>3638)
                     /locus_tag="VN034_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWV60923.1"
                     /translation="GGTRAVWRSGAIRDRDYPREASRANTGGEVEVRFTIEASGRVSG
                     CRVTQSSGDASLDETTCRLIEERFWFKPATNAAGEAIASPYGWRQTWWLERRR"
BASE COUNT          611 a         1102 c         1217 g          708 t
ORIGIN      
        1 gagcgtcatt ctgcaccggc gagatcccgg ccttcgccgg gatgacgata tgagggctca
       61 gctatccgcc cacaggcgca gcagattgtg atacaccccc gtcagttcga tcaccgaccg
      121 gtccttgccg ccgagttcgc cggtcagccg ctgcaccgcg cgatcgaggt cgaacagcgt
      181 gcgccgcttc tcggcgtcgc ggaccatcga ctggagccag aaaaaggagg cgacgcggcg
      241 gccgcgcgtg atcggggtga cgcggtgcag gctcgatgag ggatagacga cggcgtgccc
      301 ggcgggcagc ttgacgcgct gaacgccata atggtcctcg atcagcaatt cgccgccgtc
      361 gtaatcctcg ggcgcttcga ggaagatggt gatcgacagg tcgctgcgga tgcggaaatc
      421 gctgccgcgc ttgaggcgga tcgcattgtc gacatgcgtg tcgaactcct gccccgcgcc
      481 gtaacgattg aacagcggcg gatagatttt gagcgggagc gccgaagcga agaagagcgg
      541 cgagcggccg agcgcttcga ggatcaaccg gcccgcctgc ttcgccgcct cgccatcctc
      601 gggcaattgt tcgttatgct tggcgagcgc cgattggtgc cccgacgtcg cattgccgtc
      661 gacccattcg gcggcgtcga tgatcgcgcg gatgtccgcc acttcggcgg gcgaaagcag
      721 atcgggaacg gcaatcagca tcggcggtct gtccctttgc gctaccccgc cgcgctgcca
      781 caggtcgcgg gtgcgatcaa tggcgggaac ggcgcaccac cgcccccgcc atgcttcgtc
      841 agaagccgac cgacacggtc agcaccgccg accgcgcgtc gccgggggtc gcccagccat
      901 tgttgcggat gcgggtgaaa tagagcttgt cggtgaaatt cttgacattg agctgcgccg
      961 agatcgtgtc ggtgaaatca taggccagat aggcgttgtg gacccaataa tcgtccgact
     1021 ggaacagctc cggatcggcg gtggcggtgg gcaggttgaa ggtgaacttg ccctgatagg
     1081 tcgcgccata tccgatcttg agcccgaacg ggaattcgta ggtggtgaac aggctgcccg
     1141 aatgtttggg cgtgttctga agccgcgcgc cggcgaccgg atcggggaag gcgggcgagt
     1201 tggtgcaggt gccttcgccg ggattggcga ggcagaaatc ggacaccgac tgcaatatct
     1261 cgctgtcgag ataggtgtag ttggcggtga tcgaccagcc cggaagaatc tggccgaccg
     1321 cgctcagcgc gatgccgtca acgcgcgaat ggccgtcgag cacctggtcg ggcagcgtcg
     1381 gatcgcccga attgacgcga tagctgtcgc gctcgttgcg gaacgccgcg gcgctcagca
     1441 acaggcgccc gcccgcgact tcggccttca ccccgatctc gtaatttttg gcgctttcgg
     1501 gttcgacgtt gcaattgccg ttgaggccgt cgtcgttggg cagggcgcag ctgccgttga
     1561 ccgacgactg cgacggcgtt ttcgaattgc cataggcggc atagagggtg accgcttcga
     1621 ccggcttgta gacgagcccg atgcgataag agaagaggtt gtcgctatat tctctcggaa
     1681 cggtgatgcc gaggccgttg cgattccagc cgctgctctt ttcataacgg acgccgccgt
     1741 tgagctcgaa cttgccgagc ttcatcgcgt cgaacagata gacggcataa ttgtcgacct
     1801 cgccgcgctg gcgcccggtg taggtgaaat tgaccggacc gtcgtagaca ttgttgccat
     1861 aggtgaagcc cgggggcagc gcggggccga cgacgacctc gttcgggttg gcgatgttga
     1921 ccagcggata gcgcgtgtag aaaggcgttc cgtcggcgtt gcgcagcgcg ttgccgttgt
     1981 tcagcgtgta tttttcccac aatgccgacg cgccgatgac gagcgtatgc tcgatcgctc
     2041 cggtgttgaa cgtgccgcgc aggtcgacct gatcgtacat cagctcgttc gtcgaatcgc
     2101 gcagggtgcc gcgcgggccg ccgatataat aatagccgtc gggcacggtg aacggcgcgc
     2161 ccgacacggt gatcgtgcag ctgccgccgg tcggggttac gccggtcgac aggcaatagg
     2221 ttccctgcgg cggaccgacg acggtgagct ggctgacctt ctgccagcgc gcgaggttgc
     2281 ggatcgacca gctgtcgctg aaatcatgct cgaagatcgc ggtcgcctga tcgacggtga
     2341 tctcctgccg gtcgacgttg cggtaaccga aatagtcgct gcggtgggcg ccggggacga
     2401 ggccgtcgta ataggggacg ccatattgcg ggatattgtc gtcttcctga tgcagatatt
     2461 gcagcgtcag cttggtcgga cccgccatgc cgatgctgat cgacggtgcg acgccccagc
     2521 gtttgtaagt gtcgacgtcg cgtccgggga cgtcgttctt gtgcgccatc gcgttgaggc
     2581 gcaccgcgat caggtcattg agccggaggt tcgcatcgac cgtgccgcga taataattgt
     2641 cggtaccgat gcccgcggtg ccgatgacgc gcgtttcgtc gagcgggcgc ttggtgacga
     2701 tgttgatcga cccgccgacc gatcccgcac cgctttgcac cgaattggcg ccgttgacga
     2761 tttcgagctg ttcgaggttg aacgaatccg accggctata ggcggcgctg tcgcgcacgc
     2821 catcctgcgt gatatcgccg cccgccgaat agccgcggaa gttgatcgaa tcggccgggg
     2881 gcgagccgcc ttccgccgcg ccgaaggtga tgccgggcac gttcgacagc atgtcgcgca
     2941 gcgtcagcag attctgctgc tcgatcgtct cgccggtgat caccgtgacc gtctgcggcg
     3001 tgtcgcgcac cggacgcgtc gatttgggcg attcctgctg cgtctgataa tgctggccgt
     3061 tgacgatgat ctgctcgcgt tcgtcgcggc gttcgcgttc ggattgctgt tcggaagacg
     3121 catcggcacc cgtgtcggcc gccatggccg cggtgctgat caggctgccg acgcaggaga
     3181 gcgccaggaa agtggcggac gtcgtaggct tcacggctgt tacccctgtt gttatggacg
     3241 gggcgccgct attgcgagtc actcgcagag tcaattacta ttgcgaggca ttcgcgataa
     3301 ttatatgtcc ttgtcgtata tgcgcaacaa tcgagcaaat cgacctagcg ccgccgctcg
     3361 agccaccaag tctgccgcca gccatatggg ctcgcgatcg cctcgcccgc cgcgttggtc
     3421 gcgggcttga accagaaccg ctcctcgatc agccggcacg tcgtttcgtc aagcgaagcg
     3481 tcgccactcg attgggtgac gcggcatccg ctgacccggc cgctcgcttc gatggtgaag
     3541 cggacttcga cctcgccgcc tgtgttcgcc cggctcgctt cgcgcggata gtcgcggtcg
     3601 cggatcgctc cgctccgcca caccgctctg gtgccgcc
//
LOCUS       DATMSU010000364         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_129784,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000364 DATMSU010000000
VERSION     DATMSU010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129784"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VN034_09730"
     CDS             complement(<1..224)
                     /locus_tag="VN034_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60924.1"
                     /translation="MTVRNFGRQQAAAFVLAATLAAGAPVAAQDTTTSSQPPIDFRLP
                     PPTNDGRAPGVQGPSDNGLPPVAPGERRGQP"
     gene            343..813
                     /gene="queF"
                     /locus_tag="VN034_09735"
     CDS             343..813
                     /gene="queF"
                     /locus_tag="VN034_09735"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWV60925.1"
                     /translation="MTDSTPTPLAPKHLGQSSELPAAPEAAVLDYVPNPRAGELYLVR
                     FAAPEFTSLCPVTGQPDFAHLVIDYAPGETIVESKSLKLFLGSFRNHAGFHEDCTVGI
                     GRRLFDEMRPQWLRIGGYWYPRGGIPIDVFWQSGRPPEGLWLPDQGVAPYRGRG"
     gene            859..1269
                     /locus_tag="VN034_09740"
     CDS             859..1269
                     /locus_tag="VN034_09740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWV60926.1"
                     /translation="MSATALPHDHDIKVGPDVIDFMGHVNNAHYLSWVQEAVLAHWRH
                     IAPPEAVAQHLWVALKHEITYRRPAFLDDQVIATVILEKVQGARAFYETIIKRGEDVL
                     AEVKSSWCCIDAETLRPARLASEVVARFFPGEKS"
     gene            complement(1311..1544)
                     /locus_tag="VN034_09745"
     CDS             complement(1311..1544)
                     /locus_tag="VN034_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60927.1"
                     /translation="MKRLKKGAIAIAAASMVLAPVAASAAPAIDGARATSAVRGGNNL
                     EGASWIAIVLGLAIIAGGIWLVVDDDNDDPVSP"
     gene            complement(1627..2913)
                     /locus_tag="VN034_09750"
     CDS             complement(1627..2913)
                     /locus_tag="VN034_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV60928.1"
                     /translation="MTKPILPREVAAAVVDPVAYGNWDPLHDQLAWARANAPLAVAEN
                     PELDPFWLVTRHADIMAISRDPARFANGLRPTVLTNRDGEAIARAATPGGDGHLIRSL
                     VQMDAPDHMKYRLLTQSWFMPKNLKIVEDRIRHHARSAVEQMLAMDGTCDFARDVAAH
                     YPLRVIMDILGVPPEDEPRMLMLTQQLFGSTDPELNRSREAITSAEQAIAMLNYVIAD
                     FEQYFGALTADRRASPREDIATVIANAMVGGEPIPDRELAGYYMIIATAGHDTTSAST
                     AGAIMELAKNPALFARFRDAESDKAGLIEEAIRWTTPVQHFMRSAREDVEIGGQTIRA
                     GDWLMLNYVSANRDEEVFGDPFAFDPDREKNQQIAFGFGAHVCLGQHLARMEMRILME
                     ELLPRLKSLELAGEPARVESVFVGGLKRLPIRFARA"
BASE COUNT          541 a         1059 c          996 g          579 t
ORIGIN      
        1 ggctgccccc gccgctcgcc cggcgccacc gggggcaagc cattgtccga tggcccctgc
       61 acgcccggcg cacggccgtc attggtgggc ggcggcaggc ggaaatcgat cgggggttgg
      121 gacgaggtcg tcgtgtcctg cgcggccaca ggagcgcccg ctgccagcgt tgccgccagc
      181 acgaacgccg ccgcctgttg tcgcccgaag ttacgcaccg tcatcttgtc cgcgatccca
      241 cgctacccat atgtcgcccg cgccgctgaa ttggcgctga accccggccg cgccgcccac
      301 gagatcggtg acatcggccg cgctgcgcac tagagccttt ccatgaccga ttccacccct
      361 accccgcttg cccccaaaca tctcggccaa tcgagcgaac tgcctgccgc tcccgaagcg
      421 gccgttctcg actatgtgcc caatccgcgc gctggagagc tttatctcgt ccgcttcgcc
      481 gcgcccgagt tcacgtcgct ctgcccggtg accggccagc ccgatttcgc gcatctcgtc
      541 atcgattacg cgcccggcga aacgatcgtc gaatcgaaga gcctcaagct cttcctcggc
      601 tccttccgca atcatgcggg ctttcacgag gattgcaccg tcggcatcgg ccgccgcctg
      661 ttcgacgaga tgcggcctca atggctgcgc atcggcggct attggtatcc gcgcggcggc
      721 attccgatcg atgtcttctg gcaatcgggg cggccgcccg aagggctgtg gctccccgac
      781 caaggcgtcg cgccgtatcg cgggcgggga tgaagattca tctttccgtc acaatatatt
      841 gacactagtg tcaacgatat gagcgcaacc gcactgcccc acgatcatga tatcaaggtc
      901 ggtcccgacg tcatcgactt catggggcat gtgaacaacg cccattatct gagctgggtt
      961 caggaagcgg tgctcgcgca ctggcgccat atcgcgccgc ccgaagcggt cgcgcaacat
     1021 ctgtgggtcg cgctgaagca cgagataacc tatcgccgtc ccgcgtttct ggacgatcag
     1081 gtcattgcga cggtgatctt ggaaaaggtt cagggcgcgc gcgcttttta cgagacgatc
     1141 atcaaacgcg gcgaggacgt gctcgccgag gtcaaatcga gttggtgctg cattgacgcc
     1201 gaaacgctcc gccccgcgcg actggcgagc gaagttgtcg cccgcttttt tccgggcgag
     1261 aaaagttagg acccgccccc gcggggacgg gtgccttcag gcgaagagtt ttacgggctg
     1321 acgggatcgt cgttgtcgtc gtcgacgacc agccagatgc cgccggcaat gatggcgagg
     1381 ccgagcacga tcgcgatcca gctcgcgcct tcgagattat ttccgccgcg aacggccgag
     1441 gtcgcacgcg ccccgtcgat tgcgggtgca gccgaagccg ccaccggcgc aaggaccatg
     1501 gaagctgccg cgatagcgat cgcgcccttt ttcaatcttt tcatcacaaa ctcctctagc
     1561 tgccccgaaa cgcggctcgc gagcgatcgt tccattttcg caaggaaagg ttgaatccaa
     1621 aggattctag gcgcgcgcga aacggatcgg caggcgcttc agcccaccga cgaacaccga
     1681 ttcgacgcgc gcgggttcgc ccgcgagttc gaggctcttg agacgcggca gcagttcctc
     1741 catcaatatc cgcatttcca tccgcgcgag atgctggccg aggcagacgt gggcgccgaa
     1801 gccgaaagcg atctgctggt tcttctcccg atcgggatcg aaggcgaagg ggtcgccgaa
     1861 cacctcctcg tcgcgattcg ccgacacata gttcagcatc agccagtcgc ccgcgcggat
     1921 cgtctggccg ccgatctcga cgtcttcgcg cgcgctgcgc atgaaatgct ggaccggcgt
     1981 cgtccagcga atcgcctcct cgatcagccc cgccttgtcg ctctccgcgt cgcggaaacg
     2041 ggcgaacagc gcggggttct tcgccagttc catgatcgcg ccggcggtcg aggcgctcgt
     2101 cgtgtcgtgg cccgcggtcg cgatgatcat ataatagccc gcaagctcgc gatcgggaat
     2161 cggctcgccg ccgaccatgg cattggcgat caccgtcgcg atatcctcgc gcgggctggc
     2221 gcggcggtcg gcagtgagcg cgccgaaata ttgttcgaaa tcggcgatga cataattgag
     2281 catcgcgatc gcctgttcgg cgctggtgat cgcctcgcgg ctgcggttga gctcgggatc
     2341 ggtcgacccg aacaattgct gcgtcagcat cagcatgcgc ggctcgtcct cgggcggcac
     2401 ccccaatatg tccatgatca cgcgcagcgg ataatgcgcc gcgacgtcgc gcgcgaaatc
     2461 gcatgtcccg tccatcgcca gcatctgttc gaccgcgctg cgcgcgtgat ggcggatccg
     2521 gtcttcgacg atcttcagat tcttgggcat gaaccagctc tgggtgagca gccggtattt
     2581 catatgatcg ggggcatcca tctgcaccag cgagcggata aggtgcccgt caccccccgg
     2641 cgtcgcggcg cgcgcgatcg cctcgccgtc gcggttggtg agcacggtcg ggcgcaggcc
     2701 gttcgcgaag cgcgccggat cgcggctgat cgccatgatg tcggcgtggc gcgtcacgag
     2761 ccagaaggga tcgagctcgg gattttcggc gacggcgagc ggcgcattcg cgcgcgccca
     2821 ggcgagctgg tcgtgcagcg ggtcccaatt gccataggcg acggggtcga cgacggcggc
     2881 ggctacctct cggggcaata taggttttgt catgaaacca aactgccgcg aatcgcgtcg
     2941 tctgtcgata ggctatgacc gcccgtcgaa gcggcacccg cgcgaaggcc gcatcggcgt
     3001 agcggagcac gaaataaccg gggatttcat catgaaaaca actgcacgac gtttcctttt
     3061 cgccgccgct tcgtcgctcg gcctagcggc gtgtgccgcg cagcaaggcg tcgcaccgac
     3121 cgcgcccatc gcggcggcgt ccatgcttac cggcgcgccg ctcgatgcga gcatc
//
LOCUS       DATMSU010000365         4161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_129793,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000365 DATMSU010000000
VERSION     DATMSU010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129793"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..806)
                     /locus_tag="VN034_09755"
     CDS             complement(183..806)
                     /locus_tag="VN034_09755"
                     /inference="COORDINATES: protein motif:HMM:TIGR02161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HWV60929.1"
                     /translation="MIGGRLRAIWARVWGWIKPFWTTMWRPSLHFSMGFLTLGGFIAG
                     IIFWGGFNTAMEATNTEEFCTSCHEMRDNVFAELKTTIHYNNRSGVRAVCSDCHVPHN
                     WTDKIGRKMQASKEVWGKIFGSIESREKFLDKRLELAKHEWARLKANDSLECRNCHQY
                     DSMDLTRQSPRAGQIHQLYLGTGQKTCIDCHKGIAHHLPNMKGIPQG"
     gene            complement(803..1312)
                     /locus_tag="VN034_09760"
     CDS             complement(803..1312)
                     /locus_tag="VN034_09760"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF015828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HWV60930.1"
                     /translation="MKRIRALAALTVAIGLAACGSKHEETPRKAPGAPVMISAPGQID
                     ALRRGVPIDREASPLPMARVENFDRIRPVNYEMQPPTIPHAIDNYQLTVNTNRCMLCH
                     TRSNAKKFQAPPVSDAHYLTRDGKLLEQISSRRYFCVQCHVVQTDAEPLVGNRYEGLN
                     TTLAKEETQ"
     gene            complement(1309..3801)
                     /gene="napA"
                     /locus_tag="VN034_09765"
     CDS             complement(1309..3801)
                     /gene="napA"
                     /locus_tag="VN034_09765"
                     /inference="COORDINATES: protein motif:HMM:NF010055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HWV60931.1"
                     /translation="MTTRRDFIRASAVGAAASAASIAVPARFAGAATAPIPSEVKWSK
                     APCRFCGTGCGVQVAVQDNRVVATHGDADAEVNRGLNCVKGYFLSKIMYGADRLTTPL
                     LRKKDGQYSKDGDFEPVSWDEAFDVMAAEFKRVLKAKGGTGIGMAGSGQWTVWEGYAA
                     NKLMKAGFRTNHIDPNARHCMASAVTGFMRTFGMDEPMGCYDDFEAADAFVLWGSNMA
                     EMHPILWTRVTDRRLSHDHVRVAVLSTFEHRSFDLADIPIIFKPQSDLAILNYIANHI
                     IQTGRVNEDFVRKHVSFKRGNDDIGYGLRPDNPLELKAKNAKDPNGSTPIDFDAFKAF
                     VEPYTLDHAAELSGVEKGFLKHLAELYADPKVNVMSLWTMGFNQHTRGTWANNMVYNI
                     HLLTGKIATPGNSPFSLTGQPSACGTAREVGTFSHRLPADLVVTDPEHRKHAEEIWKL
                     PHGLIVDKVGYHIVEQNRMLKDGKLNAYWVMANNNMQAAANLENEGYPGYRNPDNFIV
                     VSDPYPTVTAQAADLILPTAMWVEKEGAYGNAERRTQFWHQLVKAPGEARSDLWQLME
                     FSKRFTTDECWPADLLAANPAYKGKTLFEVLYRNGKVDRFAPGEVEAGYANDEAAAFG
                     FYPQKGLFEEYAEFGRGHGHDLAPFDAYHKARGIRWPVVNGKETRWRYKEGSDPYVKA
                     GTGWQFYGNKDGRAVIWALPYEPPAEVPDADYDLWLATGRVLEHWHSGSMTMRVPELY
                     KAFPSAVVFMNPDDAKARGLKRGDEVKVISRRGEMRSRVETRGRNRVPVGLVFVPWFD
                     AAQLINKTTLDATCPISKQTDFKKCAVKVVMA"
     gene            complement(3798..>4161)
                     /locus_tag="VN034_09770"
     CDS             complement(3798..>4161)
                     /locus_tag="VN034_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HWV60932.1"
                     /translation="PDAAPAWASAPSRRSRSPHPRSSMDDEVHIASFIVRHRPDALAS
                     IERLVAETAGAEVAAREKGRCIILHECGGTRELLACMDAVQALAGVVGVSLVYHHAEP
                     RSALDDVLKPDAGEESRL"
BASE COUNT          704 a         1341 c         1321 g          795 t
ORIGIN      
        1 ggcgcagtgc gggccggtcc tggccggcgc gaccgcgaaa gcgcagcgca ccagcaaggg
       61 caaaccccgc gacgacgtcg gcaagggcac gccggcgaaa tcggaggacg gcaagcaatc
      121 atcgaaaggc aaggaaaagc agcgcaagaa attcctgggc atcttctgag cccgccggcg
      181 gctcatccct gcggaatgcc tttcatgtta ggcagatggt gggcgatccc cttgtggcag
      241 tcgatgcagg tcttctggcc ggtcccgaga taaagctggt ggatctgccc cgcgcgcggc
      301 gactggcggg tgaggtccat cgaatcatat tgatggcagt tgcggcattc gagcgagtca
      361 ttcgccttga gccgcgccca ttcatgcttc gcaagctcaa gccttttatc gaggaacttc
      421 tcgcgcgatt cgatcgaacc gaagatcttg ccccacacct ccttcgacgc ctgcatcttg
      481 cggccgatct tgtcggtcca gttgtgcggc acgtggcagt cggagcagac cgcgcgcacg
      541 cccgaccggt tgttatagtg gatcgtcgtc ttcagctcgg cgaacacatt gtcgcgcatt
      601 tcgtggcagc tggtgcagaa ttcctcggta ttggtcgcct ccatcgcggt gttgaaaccg
      661 ccccagaaga tgatgccggc gatgaagccg ccgagcgtca gaaaacccat gctgaaatgc
      721 aggctgggcc gccacatcgt cgtccagaac ggtttgatcc agccccagac gcgcgcccag
      781 atcgcgcgaa gccgcccccc gatcattgcg tctcctcctt cgccaatgtg gtgttaagcc
      841 cttcgtacct gttcccgacc aagggctcgg cgtcggtctg gacgacatgg cattggacac
      901 agaaatagcg gcgcgacgag atttgctcga gcagcttgcc gtcgcgcgtc agataatgcg
      961 cgtcgctgac cggcggcgcc tggaattttt tcgcgttcga ccgcgtgtgg cagagcatgc
     1021 agcggttggt gttcacggtg agctggtaat tgtcgatcgc gtgcgggatc gtcggcggct
     1081 gcatctcgta attgaccggg cgtatgcggt cgaaattctc gacccgcgcc atcggcagcg
     1141 gggaggcttc ccggtcgatc gggacgccgc ggcgcagcgc atcgatctgc cccggggcgc
     1201 tgatcatgac gggcgccccg ggtgccttgc gcggcgtctc ctcatgcttc gatccgcagg
     1261 ccgcgaggcc gatggcgacg gtcagcgccg cgagcgcccg gatccgcttc atgccatcac
     1321 caccttgacc gcgcatttct tgaaatcggt ctgtttggag atcgggcagg tcgcatcgag
     1381 cgtcgtcttg ttgatcagct gcgcggcgtc gaaccaaggg acgaaaacca gccccaccgg
     1441 cacccggttg cggccgcgcg tttcgacccg gctgcgcatt tcgccgcggc gcgaaatcac
     1501 cttcacctcg tcgccgcgct tcagcccgcg cgccttggcg tcgtcgggat tcatgaagac
     1561 gaccgccgag ggaaaggctt tgtaaagctc gggcacccgc atcgtcatcg atcccgaatg
     1621 ccaatgttcg agcacgcgcc cggtcgcgag ccagagatcg taatcggcgt cgggcacctc
     1681 ggcggggggc tcgtagggca gcgcccagat caccgcgcgc ccatccttgt tgccatagaa
     1741 ttgccagccg gttcccgcct tcacataagg gtcgctgcct tccttgtagc gccagcgcgt
     1801 ttccttgccg ttcaccaccg gccagcggat tccgcgcgcc ttgtgataag cgtcgaaggg
     1861 cgcgaggtca tggccgtggc cgcgcccgaa ttcggcatat tcctcgaaca ggcccttttg
     1921 cggatagaag ccgaaagccg ccgcctcgtc attggcatag cccgcctcga cctcgcccgg
     1981 cgcgaagcgg tcgaccttgc cgttgcgata gagcacctcg aacagcgtct tgcccttgta
     2041 cgccggattg gcggcaagca ggtcggcggg ccagcattcg tccgtggtga agcgtttgga
     2101 aaattccatc agctgccaca ggtccgaccg cgcctcgccc ggcgctttca ccagctggtg
     2161 ccagaattgc gtgcgccgtt cggcattgcc gtaggcgccc tccttttcga cccacatcgc
     2221 ggtcggcagg atcaggtcgg cggcttgcgc ggtgacggtc ggataggggt cggagacgac
     2281 gatgaaattg tcggggttgc gatagcccgg ataaccctca ttctcgaggt tcgccgccgc
     2341 ctgcatattg ttgttcgcca tcacccaata ggcgttgagc ttgccgtcct tcagcatccg
     2401 gttctgctcg acgatatggt agccgacctt gtcgacgatc agcccgtgcg ggagtttcca
     2461 gatttcctcg gcgtgcttgc ggtgctcggg atcggtgacc accaggtcgg cgggcaggcg
     2521 gtgcgagaag gtgccgacct cgcgcgctgt gccgcacgcc gacggctggc cggtaagcga
     2581 gaaggggctg ttgcccgggg tggcgatctt tccggtcaga agatggatat tatagaccat
     2641 attattggcc caggtgccgc gcgtgtgctg gttgaagccc atcgtccaca gcgacatcac
     2701 gttgaccttg gggtcggcgt aaagctcggc gagatgcttc aggaacccct tttcgacccc
     2761 gctgagttcg gcggcatgat cgagcgtata gggttcgacg aaagccttga acgcgtcgaa
     2821 atcaatcggc gtgctgccgt tcggatcctt cgcattcttc gccttcaatt cgagtggatt
     2881 gtcggggcgc agcccataac cgatatcgtc gttgccgcgc ttgaagctca catgtttgcg
     2941 gacgaaatcc tcgttcaccc gcccggtctg gatgatgtgg ttggcgatat agttgaggat
     3001 cgcgaggtcg gactgcggct tgaagatgat cgggatgtcg gcaaggtcaa agctgcgatg
     3061 ctcgaaggtc gagagcaccg cgacccggac atggtcgtgc gacagccgcc ggtcggtgac
     3121 gcgcgtccag aggatcgggt gcatctccgc catgttcgag ccccagagca cgaaagcgtc
     3181 ggcggcttcg aagtcatcgt agcagcccat cggctcatcc atgccgaacg tccgcatgaa
     3241 gccggtgacc gccgatgcca tacagtgccg tgcgttgggg tcgatatggt tcgtgcgaaa
     3301 ccccgctttc atcagcttgt tcgcggcata gccttcccag acggtccact ggcccgatcc
     3361 cgccatgccg atcccggtgc cgcccttcgc cttcagcacg cgcttgaact cggccgccat
     3421 cacgtcgaac gcttcgtccc agctcacggg ctcgaaatcg ccgtccttcg aatattgccc
     3481 gtccttcttg cgcagcagcg gggtcgtcag cctgtcggcg ccgtacatga tcttggagag
     3541 gaaatagccc ttcacgcagt tgagcccgcg gttgacttcg gcatcggcgt cgccgtgcgt
     3601 cgcgaccacc cgattgtcct gaacggcgac ctggacgccg cagccggtgc cgcagaaacg
     3661 gcacggcgcc ttcgaccatt tgacctcgga gggaatcggc gcggtcgcgg cgccggcgaa
     3721 gcgcgccggg accgcaatac tcgccgccga tgccgcggca cccaccgcgc ttgcccgaat
     3781 gaaatcgcgg cgcgtcgtca taagcggctc tcctctccgg catcgggctt aagcacgtca
     3841 tcgagcgcgc tgcgcggctc ggcatgatga tagacgaggc tgaccccgac caccccggcg
     3901 agcgcctgca ccgcgtccat gcaggcaagc agttcgcggg tgccgccgca ctcgtgcagg
     3961 atgatgcacc gccccttctc gcgcgccgcg acctcggcgc cggccgtctc cgcgaccaga
     4021 cgctcgatcg acgcgagcgc gtcggggcgg tgccgcacga tgaagctcgc gatatggacc
     4081 tcgtcatcca tcgatgatct cgggtgcggc gagcgcgatc gccgcgacgg ggcagacgcc
     4141 cacgcaggcg ccgcatccgg t
//
LOCUS       DATMSU010000366         1773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_34859, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000366 DATMSU010000000
VERSION     DATMSU010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34859"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1476)
                     /locus_tag="VN034_09775"
     CDS             complement(196..1476)
                     /locus_tag="VN034_09775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV60933.1"
                     /translation="MRGKRLALAMMMACAGSAAIPLPLQAQAAAPETLRIDTKRIDAA
                     LAAMVADGRAAGTSALIWQDGREVYFGTAGMADRAEKRPMRRDTIAQIYSMTKPVTGV
                     ALMQLWEQGKFRLDDPLAKYLPEYAAMRVYAGKDAAGQPRTVPAERPISVRDILRHTA
                     GFVYGAGPTPAHDAYVAADPLALTIPLAEASRRLASVPLLYQPGTQWEYSIAVDVQAA
                     LVEKLSGQPFADYVRTHIFEPLKMTETAWRQPDARLPRFAAMNVKKDGTLVQQPAAEA
                     QTLNFRDHALTPGGFGLASTLDDYSRFARMLLGKGELDGARILKPSTVELMATDQLDP
                     AITERAWLPSKGAVGFGFDFAVRKSSPQTAEENRGATGEFFWDGMASTLFWVDPANKL
                     TAVFFVQTIPYDGTLHRDFRAAVYGADYKGRPGD"
     gene            complement(1569..>1773)
                     /locus_tag="VN034_09780"
     CDS             complement(1569..>1773)
                     /locus_tag="VN034_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HWV60934.1"
                     /translation="RVLIDAQARRAIVQRDREAEGEQVAAAIDRIMASVQAGHPQWLI
                     QNGALTAAEEPEDAAPAEAPTAE"
BASE COUNT          272 a          619 c          588 g          294 t
ORIGIN      
        1 gatccctggc gagccgctcg acctcatatt tcgccgccgc cgccgagatg cccgcaccga
       61 cgaggcgcgc gtgcagcgcg gcatcgtctt cgccggccgc gaggccttcg gcgagcaggg
      121 cgcgtttgtc ggactgggcc atctcgaaac tcctcggcct cgctcaacgg caagacgatg
      181 acaaagggat gacaactagt cccccggccg ccccttgtaa tccgcaccat aaaccgctgc
      241 gcggaaatcg cggtggagcg tgccgtcata ggggatggtc tggacgaaga agaccgcggt
      301 cagcttgttc gccggatcga cccagaacag cgtcgaggcc atgccgtccc agaaaaactc
      361 gcccgtcgcg ccgcgattct cctccgcggt ttgcggcgac gacttgcgca ccgcgaaatc
      421 gaagccgaag ccgaccgcgc ccttgctcgg caaccaggcc cgctcggtga tcgcgggatc
      481 gagctggtcg gtcgccatca actcgaccgt cgagggtttg aggatgcgcg cgccgtcgag
      541 ttcgcccttg cccagcagca tccgcgcgaa acgcgaataa tcgtcgagcg tcgaggcgag
      601 cccgaagccg ccgggggtca gcgcatgatc gcgaaagttc agcgtctgcg cctcggccgc
      661 cggctgctgc accagcgtgc cgtccttctt cacattcatc gccgcgaagc gcggcagccg
      721 cgcgtcgggc tggcgccacg cggtctcggt cattttcagc ggttcgaaga tatgcgtgcg
      781 cacataatcg gcgaagggct ggcccgacag cttctcgacg agcgccgcct gcacatcgac
      841 cgcaatgcta tattcccact gcgtcccggg ctgatagagc aatggcacgc tcgcaagccg
      901 gcggctcgcc tcggcgagcg ggatggtcag cgcgagcgga tcggcggcga cataggcgtc
      961 gtgcgcgggc gtcggcccgg cgccatagac gaagcccgcg gtgtggcgga ggatgtcgcg
     1021 cacgctgatc ggccgttcgg cgggtacggt gcgcggctgg cctgccgcat ccttgcccgc
     1081 atagacgcgc atcgcggcat attcgggcag atatttcgct agcgggtcgt cgaggcggaa
     1141 cttgccttgc tcccacaatt gcatcagcgc gacgcccgtc accggcttgg tcatcgaata
     1201 gatttgcgcg atcgtgtcgc ggcgcatcgg gcgcttctcc gcgcggtcgg ccatgccggc
     1261 ggttccgaaa tagacctcgc gcccgtcctg ccagatcagt gccgaggtgc ccgcggcgcg
     1321 gccgtcggcg accatcgctg cgagcgcggc gtcgatccgc ttcgtgtcga tcctgagcgt
     1381 ttcgggggcg gcggcctgcg cctgcagcgg aagggggatc gctgccgatc ccgcacacgc
     1441 catcatcatc gccagtgcca gtcgcttgcc gcgcatatcg cctctcccct gcccggccat
     1501 tcgccgcgat aggaacgcat cccatgcgat cgggcgcgac aggtcaaccc gaataggtct
     1561 gcggacggct attcggccgt gggagcttcc gccggcgcgg cgtcttcggg ttcctcggcc
     1621 gccgtcagcg cgccattctg gatcagccat tgcggatggc ccgcctgcac cgacgccatg
     1681 atacggtcga tcgccgcggc gacctgttcg ccctcagcct cgcggtcgcg ctgcacgatc
     1741 gcgcggcgcg cctgcgcgtc gatcagcacg cgg
//
LOCUS       DATMSU010000367         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_148004,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000367 DATMSU010000000
VERSION     DATMSU010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148004"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..595)
                     /locus_tag="VN034_09785"
     CDS             complement(98..595)
                     /locus_tag="VN034_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HWV60935.1"
                     /translation="MSHADPSRQRIISVELDEGSIVWRNPDVEQERRVAIFDLIEDNK
                     FVPQRGHPDGYAGPYRLMLRVEDGRLIFEIAREDGSPLEAIILGLGRFRRPIRDYFAI
                     CDSYYQAIKTSTAQQIETVDMARRALHNEAAEMLMDRLDGKIAVDFDTARRLFTLICV
                     LHIKG"
     gene            727..2238
                     /locus_tag="VN034_09790"
     CDS             727..2238
                     /locus_tag="VN034_09790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWV60936.1"
                     /translation="MPELDLIPTPANTGDERQLPRNIEAEAAFLGAILIDNRVVEDLP
                     VALSPDHFFEPLHGRIFQQTMALIERNSIATPVTLKPYFEADEAMKAVGGVGYLAQLT
                     GSGAGLIGARDFARQIFDLALLRELAGVGRTLVDNALDTSERVDPQAQIEEAETALYR
                     VAGGEAEMGSVKNFSQASLVALQAAERALNSGGHLSGITTGIASMNAKIGGMHNSDLM
                     ILAGRPGMGKTSLATNIAYNAAERFRRDEDDGIPPEKNMGAKVAFFSLEMSADQLATR
                     VLAEQSGVSGEALRMGKISKEQFQQLSRAAQALQTLPLFIDDTPGLTIAGLRTRARRL
                     QRRHGIGFIVVDYLQLLQGSSKSGDNRVQEISEISRGLKTLAKELNVPVMALSQLSRQ
                     VESREDKRPQLSDLRESGSIEQDADMVLFVFREDYYVAAREPKRPVEGDDVKIHAQHE
                     EWAAEMERVFGLAEVIVAKSRHGSTGKVRMHFEAKTTKFSDLADDSMAYEDYE"
     gene            complement(2336..2626)
                     /locus_tag="VN034_09795"
     CDS             complement(2336..2626)
                     /locus_tag="VN034_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWV60937.1"
                     /translation="MSRGGYVYIMTNKRGGVLYIGVTADIAARVFQHKSGRGSAFCRK
                     YGLTMLVLAEEYPTIEEAIAREKAMKAWKRQWKIELIEASNPDWSDLADASI"
     gene            complement(2685..3470)
                     /locus_tag="VN034_09800"
     CDS             complement(2685..3470)
                     /locus_tag="VN034_09800"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWV60938.1"
                     /translation="MAEVKAEPRSRDRIDVASRFTQADVIRLNNLFRGQDAAEVVASV
                     IGAGLLGETAIVSSFGAESAVLLHLVAQAAPDMPVLFLDTGKHFPETLAYRDELAAKL
                     KLNIVNLTPDAEELAAKDATELRWSYDPDGCCEIRKVKPLEKALTDFDTSITGRKGFQ
                     SSTRQGLARFELDGSTGRLKFNPLANWTREMLDDYFATHDLPRHPLEAEGYPSIGCSP
                     CTSKVKPGEDPRSGRWRGWDKTECGIHTEVTPIDDDPANDPAF"
     gene            complement(3463..3885)
                     /locus_tag="VN034_09805"
     CDS             complement(3463..3885)
                     /locus_tag="VN034_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HWV60939.1"
                     /translation="MANFRYRDDQPHEEPAVTLDAFLEQPDATAVRLEAGEDARRLIP
                     FLGRLQLIEISFPRFRDGRGFSSARILREAGYKGELRAEGDVLIDLLFFMRRCGFDSF
                     SSQHSLNPADVEAALNRYSHVYQDATDEAVPIWKLRHG"
     gene            complement(3878..5512)
                     /locus_tag="VN034_09810"
     CDS             complement(3878..5512)
                     /locus_tag="VN034_09810"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWV60940.1"
                     /translation="MYQYDKYDQGMVDARVEEFRDQVRRRIAGDINDDQFKPLRLKNG
                     LYLQLHAYMLRVAIPYGTFNSEQMRKLADIARKYDRGYGHFTTRQNIQYNWIKLEEAP
                     DILAELATVEMHAIQTSGESIRNISADQYAGAAADEICDPRPWAELIRQATTFHPEFS
                     YLPRKFKIAVISAAEDRAALRWHDCALRIVRNEAGEEGFEVYAGGGMGRTPFIAYKIR
                     DFLPAQQIFSYLQAILRVWNLHARRDNIHKQRMKILVHELGEEEFRRQVEEEFQTFLA
                     LGTDFPQAEYDRIAEYFTPPPFEAGLSDEIDRGDPDFALWVDRQVVEHKAPGYAIANI
                     SLKPIGGIPGDISAEQMDLVADLAERYSFDEVRATHAQNLVLPHVRKADLYAVWQALD
                     AAGLANVNLDLVTDIIACPGLDYCSLANARSIPLAQKISERFANMERQLDIGELKIKI
                     SGCINACGHHHAGQIGILGVDRKGTENYQLLLGGSGAEDTSQAKITGPGFDEAGIVDA
                     VERTVERYRALREPGERFLDTYRRVGLDPFKEAIYG"
     gene            complement(5514..5810)
                     /locus_tag="VN034_09815"
     CDS             complement(5514..5810)
                     /locus_tag="VN034_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HWV60941.1"
                     /translation="MRILTGNDLKSGAVIWWAGRDWSLHVEDAVDVGEHGEAVLAAED
                     GARRVNGGYIVDAEATPDGPRPAHIKDRIRALGPTVRPDLTLKPADPTAGDWVI"
BASE COUNT         1029 a         1823 c         1968 g         1095 t
ORIGIN      
        1 gcggcgccga tccggcgcag cagacgcgcg atcctcgcgc gccatgccgc cgcggcctgt
       61 gccgcctttc cgcccctgat tgcccccgcc ttcatcgtca gcccttgatg tgcaacacgc
      121 agatcagtgt gaataatcgc cgtgcggtgt cgaaatcgac cgcgatcttg ccgtcgagcc
      181 ggtccatcag catctcggcc gcctcattgt gcagcgcgcg gcgcgccatg tcgacggtct
      241 cgatctgctg cgcggtcgag gtcttgatcg cctgataata gctgtcgcag atcgcgaaat
      301 agtcgcggat cggacgacgg aaacgcccga ggccgaggat gatcgcctcg agcggcgagc
      361 cgtcttcgcg cgcgatttca aagatcaacc ggccatcctc gacacgcagc atcaagcggt
      421 aggggccggc atagccgtcg gggtggccgc gctgcggcac gaacttgttg tcctcgatca
      481 gatcgaagat cgcgacgcgg cgttcctgct cgacgtcggg attgcgccag acgatcgatc
      541 cttcgtcgag ctcgaccgaa atgatccgct gtcgggaggg gtctgcgtgg ctcatgctgt
      601 ccatgcttct acttgggctg cggcgaaggg caagagggag gacttattca cagcaaccca
      661 caactatgat agcctatccg gcatcgcctg ttgcgccgcc cgaagcggca tcgcatgaag
      721 gtcgccatgc cagaactaga cctgattccc accccggcca acaccgggga tgaacgccaa
      781 ttaccccgca atatcgaggc cgaggccgcg tttctcggcg cgatcctgat cgacaaccga
      841 gtcgtcgagg atctgcccgt cgcgctgagc cccgaccatt tcttcgagcc gctccacggc
      901 cgcatctttc agcagacgat ggcgctgatc gagcgcaaca gcatcgcgac gccggtgaca
      961 ttgaagcctt atttcgaggc cgacgaggcg atgaaagcgg tcggcggggt cggctatctc
     1021 gcgcaattga ccggcagcgg cgcggggctg atcggcgcgc gcgactttgc gcggcagatt
     1081 ttcgacctcg cgctgcttcg cgagcttgcg ggcgtcgggc gcacgctcgt cgacaatgcg
     1141 ctcgacacga gcgagcgcgt cgacccgcag gcgcagatcg aggaggccga aaccgcgctc
     1201 taccgcgtcg cgggcggcga ggccgagatg ggatcggtca agaatttcag ccaagcgagc
     1261 cttgttgcgt tgcaggcggc cgagcgggcg ctcaattcgg gcggtcatct ctcgggtatc
     1321 acgaccggga tcgcgagcat gaacgccaag atcggcggca tgcacaattc ggacttgatg
     1381 atcctcgcgg gccgtccggg catgggcaag acctcgctcg cgaccaacat cgcatataat
     1441 gcggccgagc gtttccgccg cgacgaggac gacggcatcc cgcccgaaaa gaatatgggc
     1501 gcgaaggtcg ccttcttcag cctcgaaatg tccgccgacc agctcgcgac gcgcgtgctc
     1561 gccgaacagt cgggggtgtc gggcgaagcg ctgcgcatgg gcaagatcag caaggagcag
     1621 ttccagcagc tctcgcgcgc ggcgcaggcg ctccagacct tgccgctgtt catcgacgat
     1681 acgccggggc tgacgatcgc ggggctgcgc acgcgtgcgc ggcgccttca gcggcgccac
     1741 ggcatcggct tcatcgtcgt cgactatctc cagctcttgc agggatcgtc gaagagcggc
     1801 gacaaccgcg tgcaggaaat ttcggaaatt tcgcgtgggt tgaagacctt ggcgaaggaa
     1861 cttaacgtgc cggtgatggc actgtcgcag ctcagccgtc aggtcgaaag ccgcgaggat
     1921 aagcgcccgc agctctccga ccttcgcgaa tcgggctcga tcgaacagga tgccgacatg
     1981 gtgctcttcg tgttccgcga ggattattat gtcgcggcgc gcgaacccaa gcgcccggtc
     2041 gagggcgacg atgtgaaaat ccacgcacag catgaggaat gggcggccga gatggagcgc
     2101 gtcttcggcc ttgccgaagt catcgtcgcc aaatcgcgtc acggttcgac cggcaaggtg
     2161 cgcatgcact tcgaggcgaa gaccacgaag ttcagcgacc tcgccgacga cagcatggcg
     2221 tatgaggatt atgagtgagg tgacggccta gctcgtcatc ccagcgaaag ctgggatcgc
     2281 tggctgggtg aaacaacgat agcggtccca gctttcgctg ggacgacgga tttggctaga
     2341 tcgaggcgtc ggccagatca ctccagtccg gattactagc ttcgatcagc tcgattttcc
     2401 attgccgctt ccaggctttc atcgcttttt ctcgcgcgat tgcctcctcg attgttggat
     2461 attcctcggc gaggacgagc atcgttaggc cgtattttcg gcagaaggcc gagcctctcc
     2521 ccgacttgtg ctgaaaaacg cgtgccgcaa tgtccgccgt gactccaatg tagagcacgc
     2581 caccgcgctt gttggtcatg atataaacat atccgccccg gctcatgccg gcagcgtagc
     2641 gcaaggttgc cggcgatccc agctttcgct gggatgacga atatctagaa agccggatcg
     2701 ttcgccggat cgtcgtcgat cggcgtcact tcggtgtgga tgccgcattc ggtcttgtcc
     2761 cagccgcgcc agcggcccga gcgcgggtct tcgccgggct tgaccttcga cgtgcagggc
     2821 gagcagccga tcgacggata gccttcggcc tccagcggat ggcgcggcag gtcgtgcgtc
     2881 gcgaaataat cgtcgagcat ctcgcgcgtc cagttggcga gcgggttgaa cttgagccgg
     2941 ccggtcgaac cgtcgagctc gaagcgcgcg aggccctgcc gcgtcgatga ctggaaaccc
     3001 ttgcgcccgg tgatcgacgt atcgaagtcg gtcagcgcct tttcgagcgg cttgaccttg
     3061 cggatttcgc agcagccgtc ggggtcgtac gaccagcgca gttcggtggc gtcctttgcc
     3121 gcgagttcct cggcgtcggg cgtcaggttg acgatgttaa gcttcagctt cgccgcgagt
     3181 tcgtcgcgat aggccaaggt ttcggggaaa tgcttgccgg tatcgaggaa cagcacgggc
     3241 atgtcgggcg cggcttgggc aacgaggtgc agcagcaccg cgctttcggc gccgaagctc
     3301 gagacgatcg cggtctcgcc gagcagcccg gcgccgatca cgctcgcgac gacttcggcg
     3361 gcgtcctgac cgcggaagag gttgttgagg cggatgacgt cggcctgcgt gaagcgcgac
     3421 gcgacgtcga tccggtcgcg ggagcggggt tcggccttca cctcagccat gcctcagctt
     3481 ccagatcggc accgcttcgt cggtcgcatc ctgatagaca tgcgaatagc ggttgagcgc
     3541 cgcctcgaca tcggcggggt tgagcgaatg ctgcgacgag aaactgtcga agccgcagcg
     3601 ccgcatgaag aaaagaaggt cgatgagcac atcgccctcg gcgcgcaatt cgcccttgta
     3661 gcccgcttcg cgcaggatac gcgcgctcga aaagccgcgc ccgtcgcgaa agcgcgggaa
     3721 cgagatttcg atgagctgca gccggcccag aaaggggatc agccgccgcg catcctcgcc
     3781 cgcctcgagc cgcaccgcgg tcgcgtcggg ttgttcgaga aaggcgtcga gggtgaccgc
     3841 cggttcttcg tgcggctggt cgtcgcggta acggaaatta gccatagatg gcctccttga
     3901 aagggtcgag gccgacgcgg cgataggtgt cgaggaagcg ttcgccgggc tcgcgcagcg
     3961 cgcgatagcg ttcgacggtg cgctcgaccg catcgacgat ccccgcttcg tcgaagccgg
     4021 ggccggtgat cttggcctgc gacgtgtctt cggcacccga accgccgagc agcagctggt
     4081 agttttcggt gcctttgcgg tcgacgccga ggatgccgat ctggccggcg tggtggtggc
     4141 cgcaggcgtt gatgcagccc gaaatcttga tcttgagctc gccgatgtcg agctggcgct
     4201 ccatattagc gaaacgctcc gaaatctttt gcgccagcgg gatcgagcgc gcgttggcga
     4261 ggctgcaata atcgagcccg gggcaggcga tgatgtcggt gacgaggtcg agattgacgt
     4321 tcgcaaggcc cgcggcgtcc agcgcctgcc aaacggcata gagatcggcc ttgcgcacat
     4381 gcggcaggac gaggttctgc gcgtgcgtcg cgcggacctc gtcgaagctg tagcgttcgg
     4441 caagatcggc gacgaggtcc atctgctcgg ccgaaatgtc gccgggaatg ccgccgatcg
     4501 gtttcaggct gatattggcg atcgcatagc ccggcgcctt gtgttcgacc acctggcggt
     4561 cgacccacag ggcgaagtcg ggatcgccgc ggtcgatttc gtccgaaagc ccggcctcga
     4621 acggcggcgg cgtgaaatat tccgcgatgc ggtcatattc ggcctgcggg aaatcggtgc
     4681 cgagcgcgag gaaggtctga aactcctcct cgacctggcg gcggaactct tcctcgccga
     4741 gttcgtggac gaggatcttc atccgctgct tgtggatatt gtcgcgccgc gcgtggaggt
     4801 tccacacgcg caggatcgcc tgcaaatagg agaaaatctg ctgtgccggc aggaagtcgc
     4861 ggatcttgta cgcgatgaag ggcgtgcgcc ccatgccgcc gccggcatag acttcaaagc
     4921 cttcctcgcc cgcctcgtta cgcacgatgc gcagcgcgca gtcgtgccag cgcagcgcgg
     4981 cgcggtcctc ggcagccgag atcaccgcga tcttgaactt gcgcggcaaa tagctgaatt
     5041 cggggtggaa ggttgtcgcc tgccggatca gctcggccca gggacgcggg tcgcaaatct
     5101 cgtcggctgc cgccccggca tattggtcgg ccgagatgtt gcggatgctt tcgccgctcg
     5161 tctggatcgc gtgcatttcg accgtcgcca gctcggcgag gatgtcgggc gcttcttcca
     5221 gcttgatcca gttatactgg atattctggc gcgtcgtgaa atggccatag ccgcggtcat
     5281 atttgcgcgc gatgtcggcg agcttgcgca tctgctccga attgaacgtg ccatagggga
     5341 tggcgacgcg cagcatatag gcgtggagct ggagatagag gccgttcttc agcctgagcg
     5401 gcttgaactg gtcgtcgttg atgtcgcctg cgatgcggcg gcgcacctgg tcgcggaatt
     5461 cctcgacgcg cgcatcgacc atgccttggt cgtatttgtc atattggtac atgtcagatc
     5521 acccagtcgc cggccgtcgg atcggcgggt ttcaatgtga ggtcggggcg cacggtgggg
     5581 ccgagcgcgc ggatgcggtc cttgatatgc gccgggcgcg ggccgtcggg ggttgcctcg
     5641 gcatcgacga tatagccgcc gttgacccgg cgcgcgccgt cctcggccgc cagcacggct
     5701 tcgccatgct cgccgacgtc gacggcatcc tcgacatgga gcgaccagtc gcggccggcc
     5761 caccagatga cggcgcccga tttcaggtcg ttgccggtca agattcgcat gcaatatcct
     5821 tcagagagtt ggcgacgccg ggcgcgccga ggcagtcgag cgcgtcggcg cgcgccgcga
     5881 ccttgccgac gatgatcagc gcggggctcg cgacc
//
LOCUS       DATMSU010000368        10280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_101175,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000368 DATMSU010000000
VERSION     DATMSU010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10280
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101175"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..700)
                     /locus_tag="VN034_09820"
     CDS             complement(110..700)
                     /locus_tag="VN034_09820"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWV60942.1"
                     /translation="MVVDGALLASQTRLVEFSDYVRVIPSQHAATPLGCAAGHSRFGG
                     TGKTFSVLYAAHSLTTALAETVVRDRFEGQADRRLFAAELTGRSAVRLRFAEPLRLVD
                     LRTGGCIKLGISTDIAGAKCFDEAQAFSSVVYENEAIDGILYSSRLTAENCVAIFDRA
                     TSRLASDNISPLVQLQELGASLAELNGKRGQEAAID"
     gene            complement(704..3505)
                     /locus_tag="VN034_09825"
     CDS             complement(704..3505)
                     /locus_tag="VN034_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HWV60943.1"
                     /translation="MNLLDIIERELIGIGYREDAIRRDYAFSNVWGEHGATCSVAFAA
                     FTQTPPSYRSAAFAVIEASHETAADTIRERRALGAPIFLVIEPEQVTAWQVHADKAPR
                     QIATFALDSIPQFFAEHRESWAPDRIHRAKSIGHIEPRAQLDFVDLGLIPAIAGEIHV
                     KLDRLIREAVSGTREFNGDDAIRIFFRGVFRLLAAKILMDREHHRAQSWNADDVRSVL
                     DAMDDFYELGNVSQAWPPAAIEALAPVWATFRAGFNVANISADDLAHVYESTLVTDKA
                     RAEFGTHSTPRHVADYIVGRLRLWEYGASPLRVFEPFTGAGVFLGSSLRFMRDALPAD
                     WSDKKRHDVLVRCIGGAEIDPFAAEVAKLSLILADYPNANGWEIDEADLFAPDALSSR
                     LEGARIILCNPPFEAFTKREAETYPEAVAVNTSKAVYALEMALRAEPDMLGFVLPNTV
                     LVDRRYKKQRIALEQLYGEIELVALPDGIFNVSQANSALIIARKSEGPRQLLRSAVVF
                     DADKKRFAATGVPSTMRELEQERQEEPAGDLWVSPSHDLWHRLGNLPKLGSLVKASWG
                     LRWKAGQKARTSDMPGPGRELGFQDSASVHQYALGEAKWMDVRPELLLAGGNLAWDNP
                     KILCNATRLSRGYWRLAAAVDRLGRRATQQFMGLWPHEGTNVDLDALAAVINGPVVNA
                     FLTEHSFDKRFRIGKFHEAPLPAEMPPELGRLSREYAAAAARDDANPERLDDLLEAID
                     RLIFYAYGMDEADQAELLSRMGSDRPTVGRVSRRRRVRPKAEDNGPGLFHDPDIERAQ
                     GKGLGAIISRKLGESELAARIQVLPLQDWAGEVRNSGALADLLKTSPGTIEGWVAHHL
                     VIVLPGEAGEPLFPAEQFSEGRPLTGLDKLLAIVGDPNVAWAWLKTPRPAMDVPAPIA
                     WLKAGEWKRVLDLADRDFG"
     gene            complement(3502..3933)
                     /locus_tag="VN034_09830"
     CDS             complement(3502..3933)
                     /locus_tag="VN034_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60944.1"
                     /translation="MRTAFGLSKQQWRSYRGALAPLTRDQGRAACFSATDLLATSVIH
                     RLSTGLSIPLYRFTVVAEPLFQLLAACPWPQLERSILSIDIEQSWVELVDSDREMSSA
                     TVTLLIELAPMIAELRRQLLAGVPDPQRDLAFPPMIAGGRR"
     gene            4709..5356
                     /locus_tag="VN034_09835"
     CDS             4709..5356
                     /locus_tag="VN034_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60945.1"
                     /translation="MDEKLLSLPWQIQVALGCGYTAYILAYSGIRDHHKGIDTAFRAT
                     AFGLVATLVLFLTPNWRAPLAITAAFVASVATGLIWRRWGVTALQSTLRGLDVSWADD
                     MPSAWASIALQNNRHPVSQIAVHLDDGTWLNCADTRPFVDDPFGPCTLGANGDVALYV
                     THEVEPDGTSRDNPGIRHASEGVRITYVPASRVRRVAIRHAPLRPASPLEPAATE"
     gene            complement(5586..5720)
                     /locus_tag="VN034_09840"
     CDS             complement(5586..5720)
                     /locus_tag="VN034_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60946.1"
                     /translation="MSNKKVPTQTSHRSSRTGQFVTERYANQHPNTTERERIKHPERR
                     "
     gene            complement(5908..6483)
                     /locus_tag="VN034_09845"
     CDS             complement(5908..6483)
                     /locus_tag="VN034_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWV60947.1"
                     /translation="MLIGRYSAFLDANVLHPAFLRGALLWFADERLFRPVWSAHVLAE
                     WRSSVERRRKDLEPAALDRMQAAAVQAFPDAEVAGYDALIDGLSLPDPDDRHVLAAAI
                     VGRCHGIITANLKDFPEEEVARFSMEVIHPDAFIVNIIDLEENRALAACKRHRAAMSK
                     SLPTADDYLDQFERSGLIQAHQRLIGRKDLL"
     gene            complement(6484..6978)
                     /locus_tag="VN034_09850"
     CDS             complement(6484..6978)
                     /locus_tag="VN034_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HWV60948.1"
                     /translation="MGTVAVNMEELHQPSVEEIEAARNAARQLVKLDRGRTVSFVPEQ
                     GGKGDAPEPISIPVNIFRTIITMLKEMGNGNAVAVVPVSAELTTQQAADLLNVSRPHL
                     IKLLDQGELNFRMVGTHRKLPARDVLNYRDKTTAKRREALTQMGDIDQELGLIDDEQV
                     GSRD"
     gene            complement(7684..7971)
                     /locus_tag="VN034_09855"
     CDS             complement(7684..7971)
                     /locus_tag="VN034_09855"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV60949.1"
                     /translation="MMSMEKRTKRSRGTTRMDPISIRISEVVRLTGLSRTKVYALIAA
                     GDLKAAKVGRATLVFVESLRAFIESNPHCKHGGDRRSRAFKSSKLKVCEPF"
     gene            complement(8038..8265)
                     /locus_tag="VN034_09860"
     CDS             complement(8038..8265)
                     /locus_tag="VN034_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2274 domain-containing protein"
                     /protein_id="HWV60950.1"
                     /translation="MVELRIGRLPDRTPVKIAIVVLPELNDRLHAYASAYSAAYGADE
                     PVTELIPAMLAAFLDSDRAFAKTEKAPGGRK"
     gene            complement(8258..9484)
                     /locus_tag="VN034_09865"
     CDS             complement(8258..9484)
                     /locus_tag="VN034_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HWV60951.1"
                     /translation="MTDVAPRADTAPPKIDPESLVLRAAPAPVTRFRRGTVVAIAGLG
                     AIAIIGVSWLALGRPSSGTPESGEDRAPVAAKAASEVLAGAPKNYGEVPLLGPPLPGD
                     FGRPILNHQREMEMAGVNGTTDDAAVSATQAAEAERQRLAAEERAARESGVMLQNMRA
                     VSTPSTARAEAAGMAVPAAAERPDGGPGGTERKAGSAGQPNDTNEINPHRLVAPVSPW
                     IVQAGSVIAASLITGLNSDLPGLVTAQVTENVYDSITGRTLLIPQGARLIGSYDSVVA
                     FGQSRALVVWHRIILPDGSSLRIDNVPATDTAGYAGLSDRIDRHGWQLLKGVVLSTLL
                     GVGTEIGWGSSESDLVRAIRESSQQSGARAGDQIVARSLGVQPTLRVRPGWPLRIVVH
                     KDLILRPWSGGEQAGG"
     gene            complement(9481..10278)
                     /gene="trbG"
                     /locus_tag="VN034_09870"
     CDS             complement(9481..10278)
                     /gene="trbG"
                     /locus_tag="VN034_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbG"
                     /protein_id="HWV60952.1"
                     /translation="MVALAPPALAHAAPVVASANKAAAREPAASGYLNAVQVYPYAEG
                     ALYRLYTAPGRVSDIALQPGETLTSVAAGDTARWTVGDTVSGSGEARRTHILVKPFAA
                     GLATNLVIATDRRVYHVQLTSRASGAMAAIRWTYPHDELLALARRNREGEAATPVSVD
                     VRVADLDFDYTISGDKPAWRPLRAFNDGRQTWIEFPANIAAGEAPPLFVVGEKGEPEL
                     VNYRVAGRFYVVDRLFAVAELRLGARKQKVVRIVRAERRAGQKGRGA"
BASE COUNT         1926 a         3225 c         3156 g         1973 t
ORIGIN      
        1 tcgatcccac cgatatcggc gccgacgaga tcaacgcacg catggcgaag ctcgccaagc
       61 gacgagcaca gggaagagcc gcctacgccg acgctaaatc ccgcgacagc taatcgatgg
      121 cggcttcctg accacgctta ccattcagtt cggcaagcga ggcgcccagc tcctgaagct
      181 ggacaagcgg gctgatattg tccgaggcaa gacgcgatgt ggctcgatcg aaaatcgcaa
      241 cacagttctc agccgtgagc cgagatgagt agagaatgcc gtcgattgcc tcattctcgt
      301 agaccaccga ggagaaagcc tgcgcttcgt caaaacattt ggcacccgca atatcggtcg
      361 agatcccaag tttgatgcag cctcccgtcc tcagatcgac gagtcggaga ggctcggcga
      421 aacgaagccg gaccgcagag cgcccggtga gttcggcggc gaacagtcgg cgatctgcct
      481 gcccctcgaa ccggtcgcga accacggttt ccgcaagcgc ggtcgtgagg ctgtgtgcgg
      541 catagaggac agaaaaggtt ttccccgttc caccgaagcg cgaatgtccg gcagcgcagc
      601 cgagcggcgt cgccgcatgc tgcgacggga tgacccgaac atagtccgag aattcgacca
      661 accgggtctg gcttgcgagc agcgccccgt cgaccaccat ggatcaaccg aaatcccggt
      721 cagcgagatc gaggacacgc ttccattcac ccgccttcag ccatgcgatc ggagcgggca
      781 catccatcgc gggccgcggg gtcttgagcc aagcccaagc cacattcgga tcgccgacga
      841 tggcgagcag tttgtcgagc ccggtcagcg ggcgcccctc cgaaaattgc tcggcgggga
      901 agagcggctc acccgcctca cccggaagga cgatcacgag atgatgggca acccagccct
      961 cgatggtgcc gggcgatgtt ttcagcagat ccgccagcgc gccggagttg cgaacctcgc
     1021 ccgcccaatc ctgcaacggc agaacctgaa tccgcgccgc aagttcgctc tcccccagct
     1081 tgcgcgagat aatggctccc agaccctttc cctgggcgcg ctcgatatcg ggatcatgga
     1141 agagcccggg tccgttatct tcggcttttg gccggacgcg gcggcggcgc gagacgcgcc
     1201 ctaccgtcgg tcggtcgctt cccatacgcg aaagcagctc cgcctggtcg gcctcgtcca
     1261 tcccgtaagc gtagaatata agccggtcta tcgcttcgag caaatcgtcg agccgttcgg
     1321 gatttgcgtc gtccctagcg gcggcagcgg catattcccg gctgagccga cctagttcgg
     1381 gcggcatttc cgccggtagc ggtgcctcgt ggaatttgcc gatgcggaag cgcttgtcga
     1441 acgaatgttc ggttaggaag gcattgacca ccgggccgtt gatcacggcc gcgagtgcat
     1501 cgagatcgac gtttgtccct tcatggggcc acagtcccat gaactgctgt gtcgcacgcc
     1561 tccccagcct gtcgactgcc gcggcgagcc gccaatagcc ccggctcagc cgcgtagcgt
     1621 tgcacaggat tttcggattg tcccaagcga ggttgccgcc cgcgagcaga agctcgggcc
     1681 gaacgtccat ccacttggcc tcgccgagcg catattgatg gacactggcg ctgtcctgaa
     1741 agcccagctc gcggcccggg cccggcatat cgcttgtgcg cgccttctgc ccggccttcc
     1801 agcgaagccc ccagctcgcc ttgacgagcg atcccagctt cggaagattg ccgagccggt
     1861 gccacagatc atgcgaaggc gagacccaaa gatcgcccgc cggctcttcc tgccgctcct
     1921 gctcgagctc gcgcatggtc gacggtacac cggtcgcggc gaagcgcttc ttgtcagcgt
     1981 cgaagacgac agccgatcgg agcaactgcc gagggccttc ggacttgcgc gcaatgatca
     2041 gcgcgctgtt ggcttgcgag acgttgaaga taccgtcggg gagcgcaacc agttcgatct
     2101 cgccataaag ctgctcaagc gcgatacgct gcttcttgta gcggcgatcc acgagcacgg
     2161 tgttggggag tacgaagccc agcatatcgg gctcggcgcg cagcgccatt tcaagggcgt
     2221 aaacggcctt ggacgtattt accgccactg cctcgggata ggtctcggct tccctcttcg
     2281 taaaagcctc gaaaggcggg ttgcaaagga tgatgcgcgc gccttcgagg cggctgctaa
     2341 gcgcatcagg cgcgaagagg tcggcctcat cgatctccca gccattggcg ttgggataat
     2401 cggcgaggat cagcgacagt ttcgcgacct cggccgcaaa ggggtctatt tcggcgccgc
     2461 caatgcatcg tacgagaacg tcgtgccgtt ttttgtcgct ccagtcggcg ggcagcgcgt
     2521 cgcgcatgaa gcgcagcgaa gagccgagga aaactcctgc ccccgtaaag ggctcgaata
     2581 cgcgcagcgg cgaagcgcca tattcccaga gacgcagtcg cccgacgatg tagtcggcaa
     2641 catgacgcgg cgtgctatgc gtcccgaatt cggcgcgtgc cttgtcggtg accagcgtcg
     2701 attcatacac atgagcgagg tcgtcggcgg agatgttggc gacattgaag cctgccctaa
     2761 aagttgccca cacgggtgcg agcgcctcga tcgccgcggg tggccaagcc tgcgagacat
     2821 ttccaagctc gtagaaatcg tccatcgcgt cgagcacgga gcgcacatcg tcggcattcc
     2881 aagactgcgc gcggtgatgt tcgcggtcca tgaggatctt ggcggcgagc aatcgaaata
     2941 cgccgcgaaa aaatatccgg atggcatcgt cgccattgaa ctcgcgcgtg cccgagacgg
     3001 cctcccggat caggcgatcg agcttgacgt ggatttcgcc ggcgatcgct gggataagtc
     3061 cgaggtcgac gaagtcgagc tgagcgcgtg gctcgatatg gccgatcgac ttggcgcggt
     3121 gtatgcggtc gggagcccag ctttcgcggt gctcggcgaa gaactgcggg atcgaatcga
     3181 gggcgaaggt tgcgatctga cgcggcgcct tatcggcgtg gacctgccaa gccgtaacct
     3241 gctcgggctc gatgacgaga aaaatcggcg cgccaagtgc ccgccgctcc ctgatcgtat
     3301 cggccgccgt ctcgtgcgag gcctcgatta cggcaaaggc ggctgaacgg taggatggcg
     3361 gcgtctgcgt gaaggcggcg aacgcgacgg agcaggtcgc cccatgctcc ccccagacat
     3421 tgctgaacgc ataatcgcgg cggatcgcat cctcgcgata gccgatgccg atgagctcgc
     3481 gctcgatgat atcgagcaaa ttcatcgccg cccccccgcg atcatgggcg ggaatgcgag
     3541 atcacgctgc gggtctggga caccggcaag aagctgccgg cgcaactccg cgatcatcgg
     3601 tgcaagctcg atcagcaagg taacagtcgc cgacgacatt tcgcgatcgc tgtccacgag
     3661 ttctacccac gactgctcaa tatcgatgga gaggatcgat ctctcaagct gcggccatgg
     3721 acacgcggca agaagctgga acaggggctc tgccacgacg gtgaatctat aaagcgggat
     3781 cgacaggccg gtggacaatc ggtgaatcac gctcgtcgcg agcaggtcgg tggcgctgaa
     3841 gcacgccgcc ctgccttgat ctctcgtcag cggcgccaag gctccacggt aactacgcca
     3901 ttgctgcttg ctgaggccga acgccgtgcg catctgtcca gtcgtaaaga gcatggcgta
     3961 ggtgactcca aagtgttgcc cggcaacacc attgggcgtc tagcatggct ttggaaaaag
     4021 aacaaaccgc gattctctga tgtgcgttcg cgattacgga tggatacgaa cgtgcgaaat
     4081 tctggcaggc ggcaatgcca gaagcgggtg tcggcacctc gggataattt ccctagcaac
     4141 ttattccgct tatcacagat ctgctgccct ggtgcgcgcg tcgactgaat gactgcttcg
     4201 gggcaaaaga taacatacga aggaacggcc gacatgaggc gcaaagctgt cctatacgta
     4261 gggcaaacga tatccccaaa atcagatatt gaatcgcaaa tttcagccga agtcggaaat
     4321 tcatccctac gatttcaatg ggttatcttt catcacgcta ccaatgtggt agccaccgga
     4381 tgcataaaat atctagtttt ttcaatggct taaatggatt ggtagcggag gagggattta
     4441 gcgacctatg ccaaacccct gaatttgtga cactttccta atccagcccc ggaaatctgc
     4501 cccaaaaatg gtagcttttc gatgaaatgc cttggtttta cgacgggtcg aagatgatcg
     4561 atttggcggc gtccgagccc ggctcgaaca ccaccgttcc ctgtcgcatt cgacatctgg
     4621 ttgaggcggt ttgggcttct caccttgttt ccaagaggcc aaatatattt cggacgggac
     4681 gcagacatct ctgtgatagg ctcttgtgat ggacgaaaag ctgctctcgc tgccttggca
     4741 gattcaggtc gctctgggtt gcggctatac ggcctatatt ctcgcctatt cgggcatccg
     4801 cgatcatcat aaaggcatcg ataccgcctt tcgcgcgacc gcgttcggtc ttgttgcgac
     4861 gctggttctg ttcctgacac cgaactggcg cgcgccgctc gcgattactg cagcctttgt
     4921 ggcgtcggtc gcaaccggcc tgatctggcg gcgctggggc gtgaccgccc tgcagtccac
     4981 actccgcggg ctggatgtga gctgggccga cgacatgccg agcgcttggg cgtcgatcgc
     5041 gctgcaaaac aatcggcatc ccgtgtcgca gatcgccgtt cacctcgacg atgggacatg
     5101 gctgaattgc gccgacacgc gccccttcgt cgacgatcct tttggtcctt gcaccctggg
     5161 agccaacggc gacgtagcgc tctatgtcac gcacgaggtt gagccagacg gaacatcgag
     5221 ggataatccc ggcatccgcc acgcgagcga aggcgtccgc atcacctatg tcccggcaag
     5281 tcgcgtccgg agagtcgcga tccgtcacgc tccgctgcgg ccggcctccc cactggaacc
     5341 agccgcaacg gagtgatcgc ttcgtcagcc cttatgcggc gggggcggat tggagggagg
     5401 cggcgatgac ggcgccctcg ggctgttgtt cgccccgcca ccatcgccgg tgggtgcctt
     5461 cggtgtaacg gatctatgtt ccggcgttcg atcgctcatc gatatgacct ttttccttgc
     5521 tggaattgca tgcgctgttg gcgctataaa aaagggccga cgcatcgcgc cggccctttc
     5581 cgttcttacc tccgctcggg atgcttgatg cgttcccgct cggtcgtgtt cggatgctga
     5641 ttggcatagc gttccgtcac gaactgaccc gtccgcgagc tgcggtgcga ggtctgcgta
     5701 ggcactttct tattggacat ggtccaattt ccttcttcaa aatgggagga attggcctcc
     5761 cggtgaagcg gtccttaacc ttgcgtccat acaaagccgt gctattgcga cggttgctaa
     5821 cctctgatgg agcgcattcc cgcacaggga ccgcaatctg tacgaggaat ctggggaggt
     5881 ccggttgccg ccgggcctcc tcattcttca tagaaggtcc ttccttccga tcaggcgctg
     5941 gtgggcctgg atcaatccgc ttcgctcgaa ctggtcgaga tagtcgtcag ccgtcggcag
     6001 cgattttgac atcgcggccc ggtgacgttt gcacgccgct agcgcacggt tctcctcgag
     6061 atcgatgatg ttcacgatga aggcgtccgg atggatgacc tccatcgaaa atcgcgcgac
     6121 ctcctcctcc ggaaagtcct tcagattggc ggtgatgatg ccatggcatc gcccgacgat
     6181 cgcggcggca agaacatgcc ggtcgtcggg atcgggtagc gacagaccat cgatcagcgc
     6241 atcatagccc gcgacttcgg catccggaaa cgcctgtacg gcagcggctt gcatgcgatc
     6301 gagggcggcg ggctcgagat cctttcgccg ccgctcgacg ctgctgcgcc attcagcgag
     6361 aacatgcgcc gaccagacgg gccggaacag gcgctcgtcc gcaaaccaga gcaaagcccc
     6421 ccgcaggaag gcgggatgca ggacattcgc gtccaggaat gcggagtaac gcccgatcag
     6481 cattcagtcg cggctcccca cctgctcgtc gtctatcaga ccgagttcct gatcgatatc
     6541 gcccatctgg gtcagagctt cccggcgctt ggccgtcgtc ttgtcgcgat agttgagcac
     6601 gtcgcgcgca gggagcttgc gatgcgtgcc gaccatgcga aaattaagtt cgccctgatc
     6661 gagcagcttg atgaggtgcg ggcgcgagac attgagcagg tcagcagcct gctgcgtcgt
     6721 cagttcggcg ctgactggca ccaccgcgac ggcattcccg ttgcccatct ccttgagcat
     6781 cgtgatgatc gtgcgaaaaa tgttgaccgg aatgctgatg ggttcgggag cgtcgccttt
     6841 gccaccttgc tccggtacga agctcacggt tcggccccgg tcgagcttca cgagctgccg
     6901 cgcagcatta cgggcagcct cgatttcctc gacgctaggc tggtgcagtt cctccatgtt
     6961 tacagccacg gtacccattg tctttctcct tcgggacatg agtcatatag cgcataaacg
     7021 caataaatgc aataaacgaa atatccgcaa ccatcaggtc gccgctgcgt ccgggcgact
     7081 ggcggtgtcg agcatctccg cttttcagtc atctgcaggg agtcgatcaa gggcgtggtg
     7141 cgcaaaaata ctgaaataaa aatcttgacg gaaagatagt gcggaaagcg ccgggcagga
     7201 ttggctgacg gccccacctg ccctgaaaag agagccctgc cctcgcaagc tattccgcgt
     7261 tggtgcggcc gcgctgtcat ccacttgcgg ccgctggcga agtggtgcgc cccggaagat
     7321 aggctggcac ccttcaagac cggcgcaatg actgccgaag tgcatcgcca gatcgaattc
     7381 gagcacgcgg cgagatgacg atatggacga agacttccgt catccgccag acggggcatg
     7441 agagatcgaa gatcgtttcc ggctcgatat ttccggataa atttacggaa agccacgcct
     7501 cagatagttt ttgagatcgt ccttcacctg ccagaaatca cctgcgcggt atcgaggaac
     7561 agccggtcgg tcgagctagc tgaccgttgc caatcagcac ttccccgtca cgatgtgtga
     7621 tcgcaactga ccatcggcgg cggaccaggt cggcacggga gcattttcgc gtaaatctga
     7681 cctttaaaac ggttcgcaaa cctttaattt cgaactctta aaggctctgc ttcggcgatc
     7741 gccgccatgc ttgcaatggg ggttcgattc gataaaggcg cgcaggcttt cgacaaaaac
     7801 aagcgttgct cgtccgacct tggcggcctt gagatcgccc gcagcgatca aagcgtaaac
     7861 tttggtccgg ctcagaccgg tcaagcggac gacctccgat atcctgatac tgatggggtc
     7921 cattcgcgtt gttccgcggc ttcgcttcgt ccgcttttcc atgctcatca tatcggcttc
     7981 ctctgattgg atcgacgtct cgactgtccg gcgctcaagc agcgctccgg cccggcctca
     8041 ttttcggccg cccggggcct tttcggtctt tgcgaacgcc cgatcgctat ccagaaatgc
     8101 cgcgagcatc gcgggaatga gttcggtcac cggctcgtcc gcgccatagg cggccgaata
     8161 ggcgctggca taagcgtgaa gccggtcgtt gagttcgggc aggacgacga tcgcaatctt
     8221 gacgggtgtc cgatcgggca ggcgcccaat tctcagttca accacctgct tgctcccctc
     8281 cactccacgg ccgcaatatg agatccttgt ggacgacaat gcgaagcggc cagcctggcc
     8341 gaaccctgag cgtcggctgg acgccgagac tgcgcgcgac gatctgatcg ccggcgcgcg
     8401 cgccgctttg ctggctggac tcgcgaattg cccgcacgag gtcgctctcg ctgctgcccc
     8461 aaccgatttc cgtcccgacg ccgagcagcg tcgacaggac aacacccttg aggagctgcc
     8521 agccatgccg gtcgatccgg tccgacaatc ccgcgtatcc ggccgtgtcg gtggccggga
     8581 cattatcgat cctgagggac gagccgtcgg gcaggatgat ccgatgccac accaccagcg
     8641 cccggctctg gccgaacgcg acgacgctgt cgtagcttcc gatgagcctc gcaccctgcg
     8701 gaataagcag tgtgcgcccc gtgatgctgt cgtagacatt ctcggtgacc tgagcggtca
     8761 caaggcccgg cagatcagag ttcaggccgg tgatgaggct ggccgcgatc acgcttcccg
     8821 cctggacgat ccagggcgag acaggcgcga caaggcggtg cggatttatc tcgttcgtgt
     8881 cgtttggctg tcccgccgag cccgccttgc gctctgttcc gcccgggccg ccgtcaggcc
     8941 gctcagcggc tgccggtacg gccatgcccg ccgcttcggc tctggcggtg gatggcgtgg
     9001 acacggcgcg catattttgc agcatcacgc cggactcgcg cgccgcccgc tcttcggcgg
     9061 ccagtcgttg gcgctccgcc tcggcagcct gggtcgcgct cacggctgcg tcatccgtgg
     9121 ttccgttcac ccccgccatt tccatctcgc gctgatgatt gaggattggc cgcccgaaat
     9181 cgcccggaag cggggggccc agcagcggga cttcgccata gtttttcggc gcacctgcca
     9241 gcacctcgga cgccgccttg gccgcgaccg gcgcgcggtc ttccccggat tccggcgtgc
     9301 ccgatgaagg gcgacccagc gcgagccagc tgacgccgat gatcgcgatc gcgccgagac
     9361 cggcgatcgc gacgacagtg ccgcggcgga agcgcgtcac cggcgcgggc gcggcgcgca
     9421 gcacaaggct ttcggggtcg atcttgggtg gcgccgtatc ggcccggggc gcgacgtcgg
     9481 tcatgctccc ctccccttct gccccgcgcg cctttcggcg cgcacgatgc gaacgacttt
     9541 ctgcttcctc gcgccaagcc gaagctccgc gactgcgaaa aggcggtcga cgacatagaa
     9601 tcggcccgcg acgcggtagt tcacaagttc aggttcgccc ttctctccga cgacgaacag
     9661 cggcggcgcc tctcccgcag cgatgttcgc cggaaactcg atccacgtct gccgaccgtc
     9721 gttgaaggcg cgcagcggcc gccacgccgg cttgtcgccg ctgatcgtgt agtcaaagtc
     9781 gagatcggcg acccggacat ccaccgacac cggcgttgcc gcctccccct cgcggttccg
     9841 ccgcgcgagc gccagcagtt cgtcgtgcgg gtaggtccac cggattgcgg ccatcgcgcc
     9901 cgaagccctg ctggtgagtt gcacatgata gacgcgcctg tcggtcgcga tgacgaggtt
     9961 cgtggccagg ccggcagcga agggtttcac gaggatatgg gtgcgccgcg cttccccgct
    10021 tccgctgacc gtgtctccga cggtccaccg cgccgtgtcg ccggcggcca ccgacgtcag
    10081 cgtttcgccg ggctggagcg cgatgtcgct gacccgtccg ggcgccgtgt acagtcggta
    10141 gagcgcccct tcggcataag gataaacctg gacggcgttc agatagcccg acgcggcggg
    10201 ttcgcgtgcg gcggccttgt tcgcggaggc tacgaccggc gcggcgtgcg ccagcgccgg
    10261 gggcgcgagc gcaaccagac
//
LOCUS       DATMSU010000369         6412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_129916,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000369 DATMSU010000000
VERSION     DATMSU010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129916"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VN034_09875"
     CDS             <1..906
                     /locus_tag="VN034_09875"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWV60953.1"
                     /translation="ERPLDSAEQQQLDLWLEADSRHLGAYVRAQALWLDIDRIAALDG
                     GTQRDYAPPVRQRPWRRYAMAASVALAAFGGAVAYDHLAGRISTERGEVRRIALEDGS
                     ILTLNGSSAVQVRYEDDIRRIILRRGEASFDVAHNKQRPFVVSAEGLDVRAVGTEFVV
                     GIEDGGVEVTVEEGVVAVGGKASGAAQPRYIRRNEQFVAAATGPRKAVLDAADVERRI
                     AWRKGLLVFNGQQLGAAAAEVNRYSDLRVVIEDPTLARAEFMGVFKLGDAQAFAGAAA
                     QAFNGEVVRRGDELVLVRQQNSPSH"
     gene            1026..4217
                     /locus_tag="VN034_09880"
     CDS             1026..4217
                     /locus_tag="VN034_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60954.1"
                     /translation="MVGVAATMLWAAPAIAQERQRYSIPSGDAAQTVQRIAIQSGVQV
                     MVPDADLSGVTTNPVSGNYTPLEALRRMFAGKGVEVVQNRDGAIVIRRAAAADAQAST
                     VETTGEEIIVTGSRIRRPGYDTLEATIVSDAEEFRRRGYTNVIDALNDTPGFVPSDVN
                     TIGSDQRSLTAGQNFADFFGLGSQRTLTLVNGRRFVSSNTISGAGGSTTPGGQVDLNL
                     IPAGLIERVETIAIGGAPIYGADAISGTVNVILRDDFEGAELTAQVGLTEKGDAPSYT
                     FRGLVGTNFAGERGNVALGVEYNRQDGLLKSTRTGRTFMLPDPDGDPAPNQYARDLVY
                     DYMTDGGLPFTTSLGRIVDAQGTPLQFGGGGGLVPYIQGTQLSGPIYDGGDGFRFADH
                     QSLLSPTERVIVSALGHFDISSSVTLFAEGSHARSTGYELSELSVAVSPVFNTVLPVS
                     LSNPFLSDSARSTLTANGVTDNFFFARNLSDIVDRDGGLYRNRVELWRGVVGLKGQTS
                     LFGEAANWDVALNYGRSRSKATTKYINNDRFLLAVDAIEDGNGNIVCRSGGDCVPLNL
                     FGENAFSDEAAAYVIDEGSAVSLNQQFVVNANFNGSLPFRIASEAIDFNIGAEYRKEK
                     GAFTPDAILSQPSSLLGFTFVAPYAGAKGSFHTKEIYGELSVPLVSPDQDWPVIKSLS
                     VEGAARYVDHSLAGGDLTWSAGGRFSPRLPGIGDGLQFRGVYTRSIRSPGITELFSGA
                     SPTRGALNDPCDRIRYQEGNNPTVRAANCAAALQAFGTTPASFVSTTAGVSPVGTISG
                     NPNLENEKARSWSAGVVYQPTAIPGLRLAADWSEIKLTGGIQRLAIGNLLEACYDSAN
                     FPNEPSCDAFRRLSASETGPGTANPIRIAGDIADGYNSGYVNVASIKFAGLIMSAEYG
                     FALSEDPASGRLQFGAKLFHNDRYDVQTSAAAVPTSSVGTSATPKWSGQFNTAYVTDA
                     LDLSLQALWSGPAKVNILFDSEDVPDADNLIGTYWRFNATVGVKVAERFRMQLAVSNL
                     FDRKPSQAQLNSQNYGRYDLLGRRYLLTATAGF"
     gene            4303..6249
                     /locus_tag="VN034_09885"
     CDS             4303..6249
                     /locus_tag="VN034_09885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWV60955.1"
                     /translation="MRNGLLLAASLLGLAAPASADENTPKRYTIEQLMDSDVIGGLSW
                     SPDDTKLIFTSNRTGIANIYEMPSGGGTPTPLTNSVRETVRAIGYFPTDERILFSSDQ
                     GGNELAHIYVRELDGSTRDVTPGAKHVARFVEWAQDGKSFFVQTNERDPRFFDLYEIQ
                     TDGYAKTLLFENDKAYQVRAVSPDRRYVGLSRIVDNATKHCYVYDRTAAKLIQLTAEG
                     EPVACEPQVFADTGAQLFYTTDDGGEFAFLVRQDLATGRRVTVFKPEWDVQGANLSRD
                     GATLVVSVNEDARSRPYLFDAATFKPQRLADPMPGASVGLLLANHTKKALVSASNGAT
                     PGEILMLDLATGKRTPLLNARAPGVAPDDLVAGEVVRFKSYDGVTVPGILYVPKGAKK
                     GDALPAVIHVHGGPGDESRIGYRPLQQYLANHGYVVFEINNRGSSGSGRTFYHLDDRR
                     HGDADLDDVVAAKKMLADTGFVDPAKVVIQGQSYGGYMTLAGLAFRPDAFAAGVDIYG
                     VSNWPRLLANTPSWWEDLRRLLFTEVGDPEKDADYLRKISPVFHAANIKKPLLVLQGA
                     NDPRVLPVESEDIVAKVKANGVPVDYVVFPDEGHGFRKKANQIVAYRKILEFLDTHVK
                     GVPAKTAPAGTETVSPLGDFPIGQ"
BASE COUNT         1117 a         2078 c         2085 g         1132 t
ORIGIN      
        1 gagcgcccgc tagacagcgc cgagcagcag cagctcgacc tgtggctgga agccgacagc
       61 cggcacctgg gcgcctatgt ccgcgcgcag gcgctgtggc tcgacatcga ccggatcgcg
      121 gcgctcgacg gcgggacgca gcgcgactat gcgccgcccg tccgtcagcg tccgtggcgc
      181 cgctatgcga tggctgcctc ggtcgcgctg gccgcgttcg gcggagcggt cgcttatgat
      241 catctggccg gccggatttc gaccgaacgt ggcgaggtgc ggcggatcgc gcttgaggac
      301 ggatcgatcc tcaccctcaa cggcagttcg gcagtgcagg tgcgctacga ggacgatatc
      361 cggcggatca tcctccgccg cggagaagcg tcattcgatg tcgcgcataa caagcagcgg
      421 ccgttcgtcg tcagtgccga aggattggac gtgcgcgcgg tggggaccga gttcgtcgtc
      481 ggcatcgagg atggcggcgt cgaggtcacc gtcgaggagg gcgtcgtcgc cgtgggcggc
      541 aaggcgagcg gggcggccca gccgcgctat attcgccgta acgaacaatt tgtcgccgcc
      601 gcgaccgggc cgcgcaaggc ggtgctcgat gccgccgacg tcgaacgccg gatcgcctgg
      661 cgcaagggct tgctggtttt caacggccag cagctcggcg cggcggcggc cgaggtgaac
      721 cgctattccg acctccgcgt cgtgatcgag gatccgacgc tggcgcgcgc ggaattcatg
      781 ggcgttttca agctcggcga tgcgcaggcg ttcgccggcg ccgcggcgca ggcgttcaat
      841 ggcgaggtcg ttcgccgcgg cgacgagctc gttttggtgc gccagcagaa ttctccatcg
      901 cactgatagc ggatttcccc gcgccgtcgc tcgtctgctt atggaagcgc aagatcgctc
      961 cggggcatga gggaggcaag acgatgtggg ggcaagcaag ccggttgaat aagcggaccg
     1021 ctctgatggt cggggttgca gcgacgatgt tgtgggcggc gcctgccatt gcccaggaac
     1081 ggcaacgcta ttcgatcccc tcgggcgacg ccgcccagac cgtccagcgc atcgcgatac
     1141 agtcgggcgt tcaggtcatg gtgcccgatg ccgatttgtc gggtgtgaca accaaccccg
     1201 tcagcggcaa ttacacgccc ctcgaagctc ttcgccgcat gttcgcgggc aagggcgtcg
     1261 aggtcgtcca gaatcgcgac ggcgcgatcg tgatccgccg cgcggcagcg gctgacgcgc
     1321 aggcgagcac ggtggaaaca actggcgaag agatcatcgt gacagggtcg cggatccgac
     1381 gcccgggtta tgacacgctt gaagcaacga tcgtgtccga cgccgaggaa ttccggcgtc
     1441 gcggctatac caacgtcatc gacgcgctga acgacacgcc gggcttcgtg ccttcggatg
     1501 tcaatacgat cggttcggac cagcggagcc tgaccgcggg tcagaatttc gccgacttct
     1561 tcgggctcgg atcgcagcgt acgctgacgc tcgtcaacgg acggcgattc gtctcttcga
     1621 acacgatttc gggcgcgggc ggcagcacca cgcccggcgg gcaggtcgac ctcaatctca
     1681 tcccggccgg gctgatcgaa cgggtcgaga ccatcgcgat cggtggcgcc ccgatctatg
     1741 gcgccgacgc catttcgggc acggtcaacg tcattctgcg cgacgatttc gaaggcgccg
     1801 agttgaccgc gcaggtgggg ctcaccgaaa agggcgacgc gccgagctat acgtttcgcg
     1861 ggctggtcgg caccaatttt gcgggcgagc gcggcaatgt cgcgctcggc gttgaatata
     1921 accgtcagga cggtctgctg aaatccacgc ggaccgggcg cactttcatg cttcccgatc
     1981 ccgacggcga tccggcaccg aaccaatatg cccgcgacct cgtgtatgat tacatgaccg
     2041 acggcggcct gccgtttaca acgtcgctcg gccggatcgt cgacgcgcag ggaaccccgc
     2101 tccagttcgg gggcggcggc ggactcgtcc cctatattca gggcacccag ctttcgggtc
     2161 cgatctatga tggcggcgac ggcttccgtt ttgccgacca tcagagcctg ctctcgccga
     2221 cggagcgcgt gatcgtcagc gcccttggcc atttcgatat ctcgtcgtcg gtgacgcttt
     2281 tcgccgaggg gtcccatgcg cgcagcaccg gatatgaact cagcgagctg tcggtggccg
     2341 tctcgccggt cttcaacacc gttcttccgg tatcgctcag caatcccttc ctgtcggatt
     2401 cggcgcgcag cacgctgacg gcgaacggag tcaccgacaa cttcttcttc gcgcgcaatc
     2461 tcagcgatat cgtcgatcgc gacggcggtc tctaccggaa ccgggtggag ctttggcgcg
     2521 gcgtcgtggg gctgaaaggt cagaccagcc tgttcggtga ggccgccaat tgggatgtcg
     2581 cattgaacta tggccggagc cgttcgaagg cgacgaccaa atatatcaac aacgaccgtt
     2641 tcctcctcgc ggtcgacgcg atcgaggacg gcaacggcaa catcgtctgt cgttcaggcg
     2701 gcgattgcgt gccgctgaac ctgttcggcg aaaatgcctt cagcgacgag gccgcagcct
     2761 atgtgatcga cgagggaagc gcggtcagcc tcaaccagca gttcgtggtc aacgcgaact
     2821 tcaacggatc gcttccgttc cgaatcgcga gcgaagcgat cgatttcaac atcggcgccg
     2881 agtatcgcaa ggaaaagggc gccttcacgc ccgacgcgat cttgagccag ccgtcgtcgc
     2941 tgctcggctt tactttcgtt gctccctatg cgggcgccaa gggcagcttt cacaccaagg
     3001 aaatctatgg cgagctttcg gtgccgctcg tttcgcccga tcaggactgg ccggtcatca
     3061 agagcctgag cgtcgagggg gcggcgcgct acgtcgatca ttctctggcg ggcggggacc
     3121 tcacctggtc ggcgggtgga cgtttttcgc cgcggctgcc gggtatcggc gacggactcc
     3181 agttccgcgg cgtctatacc cgctcgatcc gttcgcccgg gatcaccgag cttttctcgg
     3241 gggcctcgcc gacgcgcggc gcgctcaacg atccatgcga ccggatccgc tatcaggagg
     3301 gcaataatcc gaccgtccgg gccgcgaact gcgccgcggc gctgcaagcc ttcgggacga
     3361 cgcccgcatc gttcgtgtcg acgacggcgg gggtttcgcc cgttggaacg atctcgggca
     3421 atcccaatct cgagaatgag aaggcacgtt cctggtccgc cggggtcgtc tatcagccga
     3481 ccgccattcc ggggctgcgc ctcgcggccg actggagtga aatcaagctg accggcggga
     3541 ttcagcgcct tgcgatcggc aacctgctcg aagcttgcta cgacagcgcc aatttcccca
     3601 acgagccgtc gtgcgacgcc ttccgccggc tgagcgcaag cgagaccggg ccggggacgg
     3661 ccaatccgat ccggatcgcc ggggatatcg ccgacgggta caacagcggc tatgtgaacg
     3721 tcgcctcgat caagttcgcc ggtctcatca tgtcggccga atatggtttt gccctgtcgg
     3781 aggatccggc gtcgggacgg ctccagttcg gagcgaagct gttccacaac gatcgatacg
     3841 atgtgcagac atcggcggcg gcggtgccga caagttcggt tggaacttca gcgacgccga
     3901 aatggtcggg ccagttcaac accgcctatg tcaccgacgc gctcgacctc tcgctccagg
     3961 cgctgtggag cgggccggcg aaggtcaaca tcctgtttga tagcgaggat gttcccgacg
     4021 cggacaatct gatcggcacc tattggcgct tcaatgcgac ggtcggggtc aaggtcgccg
     4081 aacggttccg catgcagctc gcggtctcca acctgttcga ccgcaagcca agccaggcgc
     4141 agctcaacag ccagaattac ggccggtacg atctgcttgg ccgcaggtat ctgctcaccg
     4201 cgaccgcggg cttctaggcg caacatagtt cggcagcgcg ccggcccttg cgggggccgg
     4261 ctcagcgccc gcgctgcccc gtaccgatag agagagaatg aaatgcgcaa cggtctgctg
     4321 ctcgccgcca gccttctcgg tctcgccgca ccggcctcgg ccgacgaaaa tacgccgaaa
     4381 cgttacacca tcgagcagct gatggattcg gatgtgatcg gcggcctctc ctggtcgccc
     4441 gatgacacga aactgatctt cacgagcaac cgcacgggca ttgccaatat ctacgagatg
     4501 ccgtcaggcg gcggcacacc gacgccgctc accaactcgg tcagggagac ggtgcgcgcg
     4561 atcggctatt tcccgaccga cgagcgcatc ctcttttcga gcgatcaggg cggcaacgaa
     4621 ctcgcgcata tttatgttcg cgagctcgac ggctcgacgc gcgacgtgac gccgggcgcg
     4681 aagcatgtcg cgcgcttcgt cgaatgggcg caggacggca aaagcttctt cgtccagacc
     4741 aacgaacgcg acccgcgctt cttcgacctc tacgagatcc agaccgacgg ctatgcgaag
     4801 acgctcttgt tcgagaatga caaggcgtat caggtccgcg cggtcagccc cgaccgccgc
     4861 tatgtcgggc ttagccggat cgtcgataat gcgacgaagc attgctatgt ctatgaccgg
     4921 accgcggcga agctcattca gctgaccgcc gagggcgagc ctgtcgcgtg cgagccgcag
     4981 gtctttgccg ataccggcgc acagcttttc tatacgaccg acgatggcgg cgagttcgct
     5041 ttcctcgtgc ggcaggacct tgcgaccggc cggcgcgtca ccgtgttcaa gcccgagtgg
     5101 gacgtgcagg gcgccaacct gtcgcgcgac ggcgcgacgc tggtcgtctc ggtcaacgag
     5161 gatgcgcgct cgcggcccta tctgttcgac gccgcgacct tcaagccgca gcgcctcgcc
     5221 gacccgatgc cgggcgcgag cgtcggcctg ctcctcgcca accacacgaa aaaggcgctc
     5281 gtgagcgcct cgaacggcgc cacgccgggc gaaatcctga tgctcgacct tgcaacgggc
     5341 aaacggacgc cgctcctcaa cgcgcgcgca cccggcgtgg cgcccgacga tctggtggcg
     5401 ggcgaggtcg tgcgcttcaa atcctacgac ggggtcacgg tgcccggcat cctttatgtg
     5461 ccgaagggtg cgaagaaggg cgatgcgctc cccgctgtca tccacgtcca cggcggcccc
     5521 ggcgacgaaa gccggatcgg ctatcgcccg ttgcagcaat atctcgccaa ccacggctat
     5581 gtcgtgttcg agatcaacaa tcgcggcagc agcggttcgg gccgcacctt ctaccatctc
     5641 gatgaccgcc ggcacggcga tgccgatctc gacgacgtcg ttgccgccaa gaagatgctc
     5701 gccgacaccg gcttcgtcga tccggcaaag gtggtgatcc aggggcagag ctatggcggc
     5761 tatatgacgc tcgccgggct tgccttccgt cccgatgctt tcgccgcggg ggtcgacatc
     5821 tatggcgtct cgaactggcc gcgtctgctc gccaacacgc cgagctggtg ggaggatttg
     5881 cgccggctgc tcttcaccga ggtcggcgac cccgaaaagg atgccgatta tctccgcaag
     5941 atttcgccgg tcttccacgc cgcgaatatc aagaagccgc tgctcgtact ccagggcgcg
     6001 aacgatccgc gcgtgctgcc cgtcgagtcc gaggatatcg tcgccaaggt gaaggcgaac
     6061 ggcgtgccgg tcgactatgt cgtcttcccc gacgaggggc acgggttccg caagaaagcg
     6121 aaccagatcg ttgcctatcg caagattctg gaattcctcg acacgcatgt gaaaggcgtg
     6181 ccggcgaaga cggccccagc cggaacggag acggtttcgc cgctcggcga tttcccgatc
     6241 ggccagtgag cgatcttcgt ctcagccgcc gcgaactgat cgccgcgggc gcgatcgctg
     6301 ccgcgacgcc ggtatgggcg gcggcgccgc gcggcgagga tgcgcgcctc gatgccttct
     6361 tcgaaagcgt cttccagcgc gatctcgcgc gcaatcccgc ggcgcaatcg tc
//
LOCUS       DATMSU010000370         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_34948, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000370 DATMSU010000000
VERSION     DATMSU010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34948"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VN034_09890"
     CDS             complement(<1..246)
                     /locus_tag="VN034_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HWV60956.1"
                     /translation="MNGDAATLWPRVAEGLRRDLGVRTFDHWLKPVRFADYCALSGVV
                     TLETVSRFSANWINERFGDRLELAWRQQLPAVRGVTVR"
     gene            complement(746..1009)
                     /gene="rpsT"
                     /locus_tag="VN034_09895"
     CDS             complement(746..1009)
                     /gene="rpsT"
                     /locus_tag="VN034_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWV60957.1"
                     /translation="MANTPQAKKRIRRNTARTVVNKNRVSRIRTLVKKVETAVAAGDK
                     DAAAAALKAAQPEMARGVAKGVLHKNTVARKYSRLTKSVNAIA"
     gene            complement(1200..2012)
                     /gene="mutM"
                     /locus_tag="VN034_09900"
     CDS             complement(1200..2012)
                     /gene="mutM"
                     /locus_tag="VN034_09900"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWV60958.1"
                     /translation="MPELPEVETTVRGLAPFLEGQRLTSVITFRPDLRRPFPADLAQR
                     LTGATVTALSRRAKYGVISTDRDDHMIFHLGMSGRWRTEGGEAGKHDHLLLETGAGHR
                     LFLHDPRRFGSVDLVSGDPLALFPAFVTLGPEPLSDAFDAAYLAKALAGRRAPIKAML
                     LDQTIVAGLGNIYVCEALNMAKIAPTKPAADVSKAKLAALVPAIKAVLTAAIAAGGST
                     LRDFLGPEGDLGYFAKDWRVYGREGEACECGGTIARIVQSGRSTFFCPKCQR"
     gene            2105..2842
                     /locus_tag="VN034_09905"
     CDS             2105..2842
                     /locus_tag="VN034_09905"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV60959.1"
                     /translation="MATPDTVSFGYEQVPAGEKTAMVGEVFSRVARKYDIMNDAMSGG
                     MHRLWKDRFVRRVKPREGETILDMAGGTGDIAFRMERFGASITVADINPDMLGVGMER
                     AAERGIDTLVWSEQNAEKLSFPDQFFDAYTIAFGIRNVTDIPAALKEAHRVLRYGGRF
                     FCLEFSTNEWPGFAQAYDAYSHHLVPKLGKLIADDEDSYRYLIESIRRFPPMPKFAQM
                     IREAGFTAVKVEPILGGLVAIHSGWKA"
     gene            2839..>2997
                     /locus_tag="VN034_09910"
     CDS             2839..>2997
                     /locus_tag="VN034_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HWV60960.1"
                     /translation="MTRPVTHIVRLLKWGRILARHGALRGIETAPQTPPGVKRLCRLA
                     RLGTIQPKI"
BASE COUNT          551 a          965 c          956 g          525 t
ORIGIN      
        1 acgcaccgtg acgccgcgca ccgcgggcaa ttgctgccgc cacgcgagtt cgagccggtc
       61 gccgaagcgt tcgttgatcc agttggccga aaagcggctg acggtttcga gcgtcacgac
      121 gcccgacagc gcgcaataat cggcaaagcg cacgggcttc agccaatggt cgaaggtgcg
      181 gacgccgaga tcgcggcgca gcccttcggc gacgcgcggc cagagcgtcg cggcatcgcc
      241 gttcatcgct ctgtcctttg cgtcatgtcc atgccgattg ccgctctctg tcacgccgtg
      301 ccgccccctc aggtcccctt tgcgccggag ttcggacggc cagccacatg ccgattcgtc
      361 gcctgcgccg ggcacaggtt ctcatccggg cgaggagccc gaaaggatgc gaatcatgtc
      421 gatgtaacgg ccgccagcga aggccgacga cgcacctgtc tagtcagggc gcggcgagtc
      481 gatcaagggc tcgccatgta aaaatactga aataaaaatc ttgactcgcc ggggccgccg
      541 gaatcgcgtc aaaacgtcat tttatcattt attatcaaat gcttatttat gacatttcgg
      601 aggccaaaca acgaatcgca gcaattccgt cacttaccgc gctgcaacac tattgccacc
      661 gggctgtcac aatatcgggc gcaaaaaaag acccgcgggc aagatgccgg cgggtccttt
      721 ttgttccctt tcgggacgtg ggcgatcagg cgatcgcgtt cacgctcttg gtcaggcgcg
      781 aatatttgcg cgcgacggtg ttcttgtgga gcacgccctt ggcgacgccg cgcgccattt
      841 cgggctgagc agccttcaac gcggcggcag cggcgtcctt gtcgccggcg gcgacggccg
      901 tttcgacctt cttcaccagc gtgcgaatgc gcgaaacgcg attcttgttc acgacggtgc
      961 gcgcggtgtt gcggcggatg cgcttctttg cctgcggcgt attggccata aattcctcta
     1021 gtctttcaaa cggtctacat ggtcggagca cagccgcgta cggagcagcc gaatcgcgcg
     1081 aaatgcagcg aatcaatgtc cggatgcggc cggttccgga cgaccggacc ctgctcgaag
     1141 ggtgcgcgca tagcgatgaa gggcgatttg gtcaatggcc ttgcgggcaa tatcccgcat
     1201 tatcgttggc atttggggca aaagaaggtc gagcggccgc tctgcacgat ccgcgcgatc
     1261 gtcccgccgc attcgcacgc ctcgccctcg cggccgtaga cgcgccagtc cttcgcgaaa
     1321 tagccgagat cgccctcggg gccgaggaaa tcgcgcaagg tcgagccacc cgccgcgatc
     1381 gccgcggtca gcaccgcctt gatcgcggga acgagcgccg cgagtttcgc cttggatacg
     1441 tcggccgcgg gcttcgtcgg cgcgatcttc gccatgttga gcgcctcgca gacatagata
     1501 ttgccgaggc ccgcgacgat cgtctggtcg agcagcatcg ccttgatcgg cgcgcgccgc
     1561 cccgccagcg ccttggcgag ataggcggcg tcgaaagcgt cggacaaggg ctcgggcccc
     1621 agcgtcacga acgccggaaa cagcgccagc ggatcgccgc ttaccagatc gaccgaaccg
     1681 aatcggcgcg ggtcgtggag gaaaagccgg tgccccgctc ccgtctcgag cagcagatga
     1741 tcatgcttgc ccgcctcgcc gccttcggtg cgccagcgcc ccgacatgcc gagatggaag
     1801 atcatatggt cgtcgcggtc ggtcgagata acgccatatt tggcgcggcg cgagagggcg
     1861 gtgaccgtcg cgccggtcag gcgctgcgca agatcggcgg gaaagggccg gcgcaggtcg
     1921 gggcgaaagg tgatgaccga ggtcagccgc tggccttcga ggaagggggc aagaccgcgc
     1981 acggtggttt cgacttcggg tagctcgggc atttcgacat ggcggtaact ggcatttgct
     2041 tgcgcgacaa ggcccggcta aaggccgtcg caacagaatt tctccccgca aaggcgcacg
     2101 ctccatggcc actcccgaca ccgtcagctt cggctatgaa caggtccccg cgggcgagaa
     2161 gaccgcgatg gtcggcgagg tgttcagccg cgtcgcgcgc aaatatgaca tcatgaacga
     2221 cgcgatgtcg ggcgggatgc accgcttgtg gaaagaccgc ttcgtgcgcc gcgtgaagcc
     2281 gcgcgaaggc gagacgatcc tcgacatggc gggcggcacc ggcgacatcg cctttcgcat
     2341 ggagcgcttc ggcgcgagca tcacggtcgc cgacatcaat cccgatatgc tcggcgtcgg
     2401 catggagcgt gcagccgagc gcggcatcga tacgctcgta tggtccgaac agaatgccga
     2461 gaagctgagc tttccggacc agtttttcga cgcctatacg atcgctttcg gcatccgcaa
     2521 cgtcaccgac atccccgcgg cgctcaagga agcgcaccgc gtgctgcgtt atggcgggcg
     2581 cttcttctgc ctcgaatttt cgacgaacga atggccgggc ttcgcgcagg cctatgacgc
     2641 ctattcgcat cacctcgtcc ccaagctcgg caagctgatc gccgacgacg aggacagcta
     2701 tcgctatctg atcgaatcga tccgccgttt cccgccgatg cccaaattcg cgcagatgat
     2761 ccgcgaagcg ggcttcaccg cggtgaaggt cgagccgatc ctcggcgggc tcgtcgcgat
     2821 ccacagcggg tggaaggctt gacccgaccc gtcacccata tcgtccgcct gctgaagtgg
     2881 gggcgcatcc tcgcacgcca cggcgcgctg cgcgggatcg aaaccgcgcc gcagaccccg
     2941 ccgggggtca agcgcctgtg ccgccttgcg cggctcggca cgatccagcc gaaaatc
//
LOCUS       DATMSU010000371        20306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_158199,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000371 DATMSU010000000
VERSION     DATMSU010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 20306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20306
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158199"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="VN034_09915"
     CDS             <1..1581
                     /locus_tag="VN034_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HWV60961.1"
                     /translation="SDRAAVRTALAELGSIDVGALSSRAALDRDVAHYVYQTLDDLLG
                     RYGYVDGNLRPSPYVVSQMNGAYYWLPDFIGSRHPIETKADVEAWLQRLAALAVALDQ
                     ESERIRHDAGIGVVPPDFVIARTIAQIKGLRDGDPVGSALIAPALKRIREKGLGDPAS
                     RASDIFRSAIQPALSRQIDALEALVPQADDRAGVWKLPDGDAYYAAAVRSNTTVDTPP
                     GELHRIGLDQCRELTAEIDLLLKAQGLSKGSVIARVSALNKDDRFRVSNDDAGREKLL
                     ALANEQLAAIKALLPGAFGITSVNPIAVRRIPPAIEAGAPGAFYSEGAPGEPGIYSLN
                     LKNPGEHVTWRLPTLTHHEGIPGHHFQYSVLGKAPALPLFRRIVRFSAYTEGWALYAQ
                     QVADELGIFKDDPFGRIGYLQSELFRAARIVVDTGLHHHRWTKAEAVAWMVENAGEQP
                     EATDREVTRYAVYPGQACSFKVGANAVYAARERARSAKGSAFDVRQFHDLVLTSGPMP
                     MTVLDASVRDWAQRVRSG"
     gene            complement(1560..1907)
                     /locus_tag="VN034_09920"
     CDS             complement(1560..1907)
                     /locus_tag="VN034_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60962.1"
                     /translation="MQALSLHGLAVVGALAVPTALLLALWESVGTALPVAWWTWLARR
                     LPDDAEADGGLMVAVVQLAITLGTAGDDLLFDAGGHRATFIASVALLAASALLAVFDA
                     RRANSDLHPERTR"
     gene            2030..5464
                     /locus_tag="VN034_09925"
     CDS             2030..5464
                     /locus_tag="VN034_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV60963.1"
                     /translation="MSLSFARSSTAPRSGVNLRAGLGLALVVLFFIISGTVAYYNIRV
                     MRDDAASIAQTHDVIASLDELFSTLQDAETSQRGFLLTGSEQYLAPYEAAVARVGSNL
                     DTVASFVRDNPRQHARMATLRRHSSSKLAELRQTIDLRRSNGLAAALDVVATNRGKGD
                     MDAIRAQLDLLRQDAERLRQVRIDEMAVAHRTAWVSAVLSCLLGIILTFAVGFLIRRS
                     SAARELQQWHQTGQIGLAAAMLGALKTEELGENILTFLTKYLGANGGAIFAGDGAQYR
                     RIGALGVPADATIVERFALKEGLLGQAAADRDVSLLSDIPDGYLTIGSALGQDKPRHL
                     AIAPAAADDVVQGVVELGFLQPVGVHALAFLDQASAPIGVALRSARFRAELQDLLEET
                     QRQSEELQVQSEELRVSNEELEEQGRALKESQVRLEQQQAELEQTNVQLEEQAERLEA
                     QRNSLAESKTAVELKARELEQASRYKSDFLANMSHELRTPLNSLLILSKLLGDNPDGN
                     LTDEQVKYADTIKASGNDLLTLINDILDLSKIEAGHVEVQPEAVPIERLATDLRQMFQ
                     PVASERGLQFDIMVAADCPATIDTDRQRLEQILKNLLSNAFKFTEKGSVGLSVERAGT
                     DRLELAVTDTGIGLAKDHQQEIFNAFHQADGSISRKYGGTGLGLSISRELARLLGGSI
                     TLSSQPGEGSRFALSVPLSYDPALAASHDAPAVPPREVTAATASQKPAKPRTRSGKVS
                     DDRHALNSSGRILLVIEDDETFARIVCDLSHEMGFQCIVAGTAEEALRLAREFKPNAV
                     VLDVGLPDQSGLAVLDQLKRDVATRHIPVHVVSGSDHTQTALSLGAIGYVIKPVEREA
                     LAGVLETLGAQLARTVRRVLIVEDDPVQREAVTRLLAAGDVETVGVGTAAECLEQLKQ
                     ETFDCMVLDLTLPDASGFELLETLHEDEAHGFPPVIVYTGHELSANDEQHLRRYSDSI
                     IIKGARSPERLLDEVSLFLHQVVTELPIEQQKMIQKARHRDAILEGRRILVVEDDVRN
                     VYSLTNILEPRGAVIEIARNGREAIEALEKSANGDGSATDLVLMDVMMPVMDGLTATR
                     EIRKDARWKKLPIITLTAKAMPDDQQNCIEAGASDYMAKPIDVDKLLSLVRVWMPR"
     gene            5472..6296
                     /locus_tag="VN034_09930"
     CDS             5472..6296
                     /locus_tag="VN034_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HWV60964.1"
                     /translation="MTQETDDIEIQLLLEALYQRYHYDFRNYARASIKRRLRQAREQM
                     NFANFTAMQESLLHDPAMLQRLLGYLTVQVSEMFRDASYFRAIREKVVPHLRTYPSLK
                     VWIAGCSAGEELYSLVILFREEGLEERTLFYATDVNQDALDKASKGIYALDRMKAFTE
                     NHRASGGKSSLSDYYSTAYGNAVFDKSLRDRVVFSDHSLVTDAVFAEMHLVSCRNVLI
                     YFNRELQDRAIGLFRDSLIRNGFLGLGSKENMRFSAHADAFTDFVREEKIYQRRDA"
     gene            6293..6868
                     /locus_tag="VN034_09935"
     CDS             6293..6868
                     /locus_tag="VN034_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HWV60965.1"
                     /translation="MSEVAARCVVIGASAGAVQALSEILPALPADFPLPILVVVHIPS
                     DRQNILPSLLQAKCRMTVREAEDKEPALAGVVYFAPPDYHLLAEADGSLSLSSDEEVL
                     YSRPSIDVLFESAADAYGAALIGIVLTGANPDGAKGLKAIADAGGTALVEDPAAAYAA
                     EMPTAALKECAAARVVPLAEMAPNLMKECGV"
     gene            6865..8046
                     /locus_tag="VN034_09940"
     CDS             6865..8046
                     /locus_tag="VN034_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HWV60966.1"
                     /translation="MNSNMDPVHFLLVDDLEENLVALEALLRRDDLVQLKARSGEEAL
                     ELLLRHDVALALLDVQMPGIDGFELAELLRGNARTRHIPIIFLTAGSADRQRRFRGYE
                     AGAVDFIQKPLEADILRSKANVFFDIYRQRQQIAAQRDELEIQTETLKAFEQLTRQQL
                     VQEQEIARFREQFIAVLGHDLRNPLASIGGGARVLRRQSLNDKALKIIDLMEASVDRM
                     AELIDNVMDFARGRLGGGIALDRQPTELDPVLRHVVNELQASEPDRKMMCTFALPTPI
                     CVDPGRIGQLASNLLANAVVHGDPRSPIELRAVLEDGEFILSVRNAGPKIPEAAMERL
                     FQPFVRGEVRPNQRGLGLGLHIASEIAKAHGGTLSVTSTEGETRFTFTMPAEQELETA
                     E"
     gene            complement(8231..9037)
                     /locus_tag="VN034_09945"
     CDS             complement(8231..9037)
                     /locus_tag="VN034_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HWV60967.1"
                     /translation="MLVKGGSVFQSLKGRLTLDHRVLKLENAVADLERGRMTGRVTID
                     SRKDTPTLSTELRVRGASFDAFVGQPDMIRGPIDGLIRINGRGDTIRQAFASGNGKIA
                     FVANGGAMNRAAAFVLGQDLGGAIGQKLGDDEKMVPIRCAIMAFSARGGVLVPAPFTI
                     DTTISRGNGRGQINLDGETVALTLAGTAKKKASLRLVDPLRIDGTLTQPDIGFDQGSK
                     TQGGDGKGLLSTIGRSIGTALGLRKDKDADGAGAIAAAIDCEKLSRAALN"
     gene            9008..9970
                     /locus_tag="VN034_09950"
     CDS             9008..9970
                     /locus_tag="VN034_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60968.1"
                     /translation="MEHRTALDQQAGDRKADYAVGRTHFAEIDPRVGNDARADACLLR
                     ACGGEARVVGKIVEIELRRVDGRIDLRPPAAFKDRRLRAKARASDRAGERVDEPVFAV
                     MAHLSPKIDILRPEQPCFDDVIELLEIRAAGSHFHAHVAQVESACHRAFGVKVEDGGC
                     EAGRKGPRGRCAFSGRSATAHGDASRRAIKVSARVYSEASARLGLDRPLEVEPAFCVA
                     EGEAAIFDRQVFEGGRIVFAALILVGAASPIAVAFTVADEDEVEAFDVERADIGVARK
                     KGEDRDLRADAHHLEHFAFAGPSRLGDLQVAHHDRGREREEFVP"
     gene            complement(10261..10458)
                     /locus_tag="VN034_09955"
     CDS             complement(10261..10458)
                     /locus_tag="VN034_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWV60969.1"
                     /translation="MNSDMLKGNWEQVKGHAQKKWGELTNDELDEINGNRRILAGKIQ
                     ERYGRARDEAEREIDEFETSY"
     gene            complement(10550..12691)
                     /locus_tag="VN034_09960"
     CDS             complement(10550..12691)
                     /locus_tag="VN034_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV60970.1"
                     /translation="MRTAIALLITTACLISTAAHAQDGTAPAAEDDRFALGQIVVTAP
                     RSEGIGIDGETLSSEAIYKFNRTALDDAVNLMPGVVAGNSGGTRNERLVFVRGFDRFQ
                     VPLSIDGIRVYLPADNRLDYGRFLTTDIAEVQVAKGYASVLDGPGAMGGAINLVTRRP
                     TKALDIDVRGTVNFDRDADYAGYSASGMIGTRQDRWYAQASYARSFTDHWDLPGNFTP
                     VVPTIEDGGERDFSRTRDWRANIKLGFTPNDTDEYAISYTKQAGAKNAPLHISDTVSA
                     PRFWNWPKWDIESVYFLSTTALGDRGTLKTRAYYNQFDSILRSFNDRTQTTQSRPFAF
                     DSPYFDKAWGGSAQLDVKLSEADTLRLAFHYRHDKHVEAQTSFSTAGVPSTEPPQTQT
                     ENSFSLALENELALTSALRFTLGGSFDWRDLKRAEEFGSPLGTTLPAVLYQYPRRDAD
                     TWNLQGRFDWNASDSLTLRASLSSRARFPTIFERFSQRFNTAIPNPALRPERATNAEI
                     GGSFTAGSIRLDGAIFYSWVEDAIFGVPTPAYPCTASTTPPATPVPGCTLRNLTQSQN
                     VGSGDYYGAEASLSATLAPGFDVGANYTYIKRDLSYAANPGFRPTGVPTHKGLIYADW
                     APLEGLHIQPSIELASSRWTLFTATPANQPARYYRTGSYTNASLRISYAVTDEIELGV
                     GARNLFDEEYSLTDGFPEPGRSLYVTLRAQF"
     gene            12769..13293
                     /locus_tag="VN034_09965"
     CDS             12769..13293
                     /locus_tag="VN034_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60971.1"
                     /translation="MLRLLLVSGVAPLFLVAGAAASPPVPEAACTAPGELPAALASWA
                     SPAFLKAAPNYKSSRHSPLIPGKAAVISLLQTPKVDYPVRPEKPGGTVSFGGVLRFTV
                     PDEGVWRVALSTAAWVDVVKDGQASTSIAHGRGPACTGIRKMVDYRLAPGTYALQIAA
                     NGSETVTLMVAHLP"
     gene            13290..14270
                     /locus_tag="VN034_09970"
     CDS             13290..14270
                     /locus_tag="VN034_09970"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWV60972.1"
                     /translation="MRLLRAAAALLLVAATGPAWTWNLPEGVGAPAIPAGNAMGAAKV
                     ELGRRLFYDADLSADGTLACAGCHEQKRAFADGNATRPGVDGSPGRRNAPGLANIAWF
                     DRLTFADPAATTLERQALIPLFGEHPVEMGMKGREAEFERRLGADRCYRRMFRNAFPE
                     KRGKIDVESVTAALAAFQRTMTSYRSPYDLYRAGEAGALPETAQRGERLFRARGCTAC
                     HAGPNLSDMRYHRLEPARAADPGLIEKTGQPDDAGRFRTPSLRNAAVTGPWWHDGSER
                     NLDGAISRHPNPVSAEEASAIGAFLSALTDPHFIADPRLSRPDTACGKPL"
     gene            complement(14265..14654)
                     /locus_tag="VN034_09975"
     CDS             complement(14265..14654)
                     /locus_tag="VN034_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV60973.1"
                     /translation="MAPTPPLKIKIQIYCGDEIAMGPGKADLLDAIREHGSISAAGRA
                     LGMSYRRAWLLVDAMNRCWKDRLVDTVPGGGQTRGARVTDVGEAILSAYRSMQKATGR
                     AASPIMFQELDALLLAEPMPVDGTPTS"
     gene            complement(14772..15824)
                     /locus_tag="VN034_09980"
     CDS             complement(14772..15824)
                     /locus_tag="VN034_09980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWV60974.1"
                     /translation="MRILIVTDAWEPQVNGVVRTLQATIAELRAAGHEVGIISPDLFR
                     SIPCPSYAEIRLAFAGRRAIGRRIRAFAPQAIHISTEGPLGLAARRWCLQNDFPFTTA
                     YHTRFPEYVAARLPVSPALVWRFIRWFHRPAQHIMVATRSLARELAGQGLTRTMMWER
                     GVDHALFRPCRTPHPAYAGLARPIQLYVGRVAVEKNIGAFLDSDRPGSKVVVGEGPAL
                     AELKARHPNALFLGKLGGEALAAAYAGADVFVFPSRTDTFGLVVIEALSCGTPVAAYP
                     VPGPGDIIREGAGALDEDLGRAIDAALLCDRGDAAALGARYGWPNCTAQFVKALTFLP
                     AEPAAGAAAGTMPRLA"
     gene            complement(15821..16693)
                     /locus_tag="VN034_09985"
     CDS             complement(15821..16693)
                     /locus_tag="VN034_09985"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWV60975.1"
                     /translation="MASISTLPIPARFPDPFTTDPHIPERVIGERRSYRTIWVSDIHL
                     GTRGCNAAMLIDFFDHVDCETLYLVGDIIDGWRLKKRHFWPPEHNDVVWRVLKRAKRG
                     TRVVYVPGNHDEMCKPFDGFDFGGVEIRREAIHETADGRKLLVVHGDEFDAVMLAHRW
                     LAFVGDAAYTALMKCNVVVNRIRSAFGLPYWSLSMVAKHKVKNAVQFIGRYEEVVAHA
                     ARSRGVDGVVCGHIHSAEMREIEGTEYYNDGDWVEGCTALVEHHDGRMEILHWAEEVA
                     ARSAPTKLALPAAA"
     gene            complement(16845..18278)
                     /locus_tag="VN034_09990"
     CDS             complement(16845..18278)
                     /locus_tag="VN034_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV60976.1"
                     /translation="MKLKDVYPLYLNNKAVQPNTDLAVTDKFTGEVAFRTALATPDVI
                     DEAIAGAVRAAEPMARLASFEKRDVLTHCVGRFQERFDELAYALCVEAGKPIADSEGE
                     VTRLIDTFRIAAEEATRNYGEVQPLDISARAKGYMGMWKRVPIGPCSFISPFNFPLNL
                     AAHKIAPAIAMGCPFVMKPASLTPLGAIIMGEVLAECDILPEGAFSILPATREGADLF
                     TTDERLKLLSFTGSPGVGWDLKAKAGKKKVVLELGGNAAVIVDKDADLDHALARIIFG
                     AFYQSGQSCIGVQRIIIHADIYDKFRDMLVAKTKKLVAGDPKSRDTFIGPMISEKEAA
                     RLDDWIQAAVAGGAKLLVGGKRDGAMLQATLLEDVDRGADAYREEAFGPLAILSSFTD
                     WDAALAEINDSKFGLQAGLFTRDLHKVLEAWDHLDVGGIVVNDVSSYRVDNMPYGGVK
                     DSGLGREGIRFAMEDMSEIRNLVIRRT"
     gene            complement(18378..20024)
                     /locus_tag="VN034_09995"
     CDS             complement(18378..20024)
                     /locus_tag="VN034_09995"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HWV60977.1"
                     /translation="MKKASDLFIDCLEEEGVEYIFGVPGEENLDFLDSLSRSKKIKLI
                     LTRHEQGAGFMAATYGRHTGKTGVCLATLGPGATNFVTAAAYAQLGGMPMMMITWQKP
                     IKKSKQGRFQILDVVAMMGPITKYTHQMASSDNIPSRVREAFRLAEEEKPGAVHIELP
                     EDIADEHTDSTPLKRSHSRRPNADSKSIREAVKALEKAKSPVLVIGAGANRTMTSCML
                     LQFIEKTGIPFLTTQLGKGVIDERHPKFLGCAALSSGDFVHRAVEASDCIVNLGHDVI
                     EKPPFFMQRGGPTVIHVSTKTAEVDPVYFPDIEVIGDIANAVWQIKEDIVPNGGWKFD
                     HLLAYRKAEVEHTESLAKDERFPIFPPHLVQSVRDAMPDDGIICLDNGVYKIWFARGY
                     TACKPNTVLLDNALATMGAGLPSAMMSAMLYPDRKVMAICGDGGFMMNSQEMETAVRL
                     GLNITVLILNDNSYGMIRWKQANMGFKDWGLTYGNPDFVKYAEAYGAHGHRVESAAHL
                     KELLAHTRDTPGVHLIDCPVDYSENDQILNNDIKRLSKEL"
BASE COUNT         3588 a         6343 c         6606 g         3769 t
ORIGIN      
        1 agcgaccggg cagccgttcg cacggcgctg gctgagctcg gttcgatcga tgtcggcgcc
       61 ttgtcttcgc gcgcggcact cgaccgcgat gtcgcacact atgtctacca gacgctcgac
      121 gaccttctcg gccggtacgg ctatgtcgac ggcaatttgc ggccgagccc ctatgtcgtc
      181 agccagatga acggtgctta ttactggctg cccgatttca tcggcagccg ccatccgatc
      241 gagaccaagg cggacgtcga ggcgtggctc cagcgcctcg cggcgctcgc ggtcgcgctc
      301 gatcaggaga gcgagcgcat ccgccacgat gccggtatag gtgtcgttcc gcccgatttc
      361 gtgatcgccc gcaccattgc gcagatcaag gggctgcgcg atggcgatcc ggtgggatcg
      421 gcgctgatcg caccagcgct caagcgcatc cgcgagaaag ggctgggcga tccggccagc
      481 cgcgcgagcg atattttccg ctcggctatc cagcccgcgc tcagccgcca gatcgacgcg
      541 ctcgaagcgc ttgtgccgca agccgacgac cgcgccgggg tctggaagct gcccgacggc
      601 gacgcctatt atgccgcggc ggtgcgttcc aatacgacgg tcgatactcc gcccggcgaa
      661 ttgcaccgca tcgggctcga tcaatgccgc gaactcaccg ccgagatcga cttgctgctg
      721 aaagcgcaag gtctgagcaa gggaagcgtc atcgcccgcg tttcggcgct caacaaggat
      781 gaccgcttcc gtgtgtcgaa cgacgatgcg gggcgcgaaa agctgctggc gctggcgaac
      841 gagcagctcg cggcgatcaa ggcgctgttg cccggcgcgt tcgggatcac atcggtcaat
      901 ccgatcgccg tgcgccgcat cccgccggcg atcgaggcgg gcgcgcccgg tgccttctat
      961 tccgaaggcg cgccgggcga gccgggcatc tattcgctca acctcaagaa tccgggcgag
     1021 catgtgactt ggcgcctgcc gaccctgacg caccatgaag gtattcccgg ccatcatttc
     1081 cagtacagcg tgctcggcaa ggcgccggcg cttcccctgt tccggcgcat cgtgcgtttt
     1141 tcggcctata ccgagggctg ggcgctctat gcgcagcagg tcgccgacga actcggcatc
     1201 ttcaaggacg atccgttcgg acggatcggc tatcttcagt ccgagctgtt ccgggctgcc
     1261 cgcatcgtcg tcgataccgg gctccatcat caccgctgga ccaaggcaga ggcggtggca
     1321 tggatggtcg aaaatgccgg cgaacagccc gaggcaaccg accgcgaggt cactcgctac
     1381 gctgtctatc caggccaggc atgcagcttc aaagtcggcg ccaacgcagt ctatgcggcg
     1441 cgcgagcgcg cacgttcagc caagggttcg gcatttgacg tgcgccagtt tcacgatctg
     1501 gttctgacct caggaccgat gcccatgacg gtactcgatg cctccgtgag agattgggct
     1561 cagcgcgttc gttcgggatg aagatcgctg ttggcccggc gcgcgtcgaa aacggcgagc
     1621 agtgcggagg cggcgagcag cgccacgctg gcgataaagg ttgcgcgatg cccgcctgca
     1681 tcgaacagca aatcgtcgcc tgcagtaccc agcgtgatcg ccagctgcac caccgcgacc
     1741 atcagaccgc cgtcggcctc ggcatcatcg ggcaggcgcc gtgcgagcca ggtccaccat
     1801 gcgacgggaa gcgcggtgcc gaccgattcc caaagtgcca gtagcagggc ggtaggaaca
     1861 gccagggcgc cgaccaccgc cagcccgtgg agcgaaaggg cttgcacgga gcaagctctc
     1921 ccggcaaggt cgctggcaca cggtagacgt ttcaagcgta aagcgtttgc gtcatttgca
     1981 aaatcgccga tatgccgacc gggccctctc cagcatagag atgtaaatga tgtcgctttc
     2041 ttttgcgcgc agttcaacgg cgccgagatc cggggtgaac ctgcgggccg gattgggtct
     2101 ggcgctggtc gtcctgttct tcatcatcag cggtacggtc gcatattaca acatccgggt
     2161 gatgcgcgac gacgccgcaa gtatcgcgca aacgcatgat gtgatcgcgt cgctcgacga
     2221 gctgttttcg acgctgcagg acgccgaaac cagccagcgc ggtttcctgc tgaccggcag
     2281 tgagcaatat ctggcgcctt acgaagcagc cgtagcccgt gtcggatcga acctggatac
     2341 ggtcgcttcg ttcgtccgcg acaatcctcg ccagcatgca aggatggcga cgcttcggcg
     2401 ccattcgagt tcgaagctcg ccgaacttag gcaaaccatc gacttacgcc ggagcaacgg
     2461 gctcgccgcg gccctcgatg tcgtcgcgac gaaccgcggc aagggcgata tggacgcgat
     2521 ccgcgcgcag ctcgacctgt tgaggcagga tgccgaacgg ctgcgccagg tccggataga
     2581 cgaaatggcg gtcgcgcatc gaaccgcgtg ggtcagcgcc gtgctctcgt gcctgctcgg
     2641 catcatcctg acgtttgccg tcggctttct catccgcagg tcgtccgccg cgcgcgaatt
     2701 gcagcaatgg catcagaccg gccagatcgg tcttgccgcc gcgatgctcg gggctctgaa
     2761 aaccgaggag ctgggtgaga atattctgac gtttctcacc aagtatctcg gcgcgaatgg
     2821 cggcgccatc ttcgcgggcg acggcgcgca gtatcgccgc atcggggcgc ttggcgtgcc
     2881 ggcggacgcg accatcgtcg agcgattcgc gctcaaggag gggcttctcg gccaggcggc
     2941 ggccgaccgg gacgtgtcgc tgctcagcga catacccgac ggctatctga ccatcggctc
     3001 ggcgctgggc caggacaagc cgcgccatct ggcgatcgcg ccggctgccg ccgacgacgt
     3061 ggtgcaaggc gtggtggaac tcggtttcct gcaaccggta ggcgtgcacg cgcttgcctt
     3121 tctcgatcag gcctccgcgc cgatcggggt cgccctacgc tcggcgcggt tccgcgccga
     3181 gttgcaggat ctgctcgaag agacccagcg ccagtcggaa gagctgcagg tgcagagcga
     3241 ggagcttcgg gtttcgaacg aagagctcga ggagcagggg cgcgcgctta aggaatcgca
     3301 ggttcgcctg gagcagcagc aggccgaact cgaacagacc aatgtgcagt tggaagaaca
     3361 agccgagcgc ctggaagcgc agcgcaacag tttggccgaa tcgaaaaccg ccgtggagct
     3421 gaaggcgcgc gaactcgaac aggcgagccg ctacaagtcc gatttcctcg cgaacatgtc
     3481 gcacgaactc cgcacgccgc tcaattcgct gctcatcctg tccaaacttc ttggggacaa
     3541 tccggacggg aacctgaccg acgagcaggt caaatatgcc gataccatca aggcgtcggg
     3601 caatgacctg ctgaccttga tcaacgatat cctcgacctt tccaagatcg aggcggggca
     3661 tgtggaggtg cagcccgagg cggtgccgat cgagcggctg gcgaccgacc tgcggcagat
     3721 gttccagccg gtggccagcg aacgcggctt gcagttcgat atcatggtcg ccgccgattg
     3781 cccggccacg atcgacaccg accgtcagcg gctcgaacag atattgaaga acctgttgtc
     3841 caacgccttc aagttcaccg agaaaggcag cgtcgggctc tcggtcgagc gcgccggcac
     3901 cgatcgcctc gaattggccg ttaccgacac cggcatcggc ctcgcgaagg atcatcagca
     3961 ggaaatcttc aacgccttcc atcaggcgga cggttcgatc agccggaaat atggcggcac
     4021 cggcctcgga ctctcgattt cgcgcgagct ggctcgcttg ctcggcggaa gcatcacgct
     4081 cagcagccag ccgggcgaag gcagccgctt cgcgctttcg gtgccgctgt cctacgaccc
     4141 cgcgctggcc gcgtcgcacg acgcacccgc cgtgccgcct cgcgaggtta cagccgcaac
     4201 cgcgtcgcaa aaacccgcga agccgcggac gcggtccggc aaggtctccg acgaccggca
     4261 cgcgctcaac agttcgggcc gaatcctgct cgtcatcgag gatgatgaaa ccttcgcccg
     4321 catcgtctgc gacctgtcgc acgaaatggg cttccagtgc atcgtcgccg ggaccgccga
     4381 ggaagccttg cggctagcgc gcgaattcaa acccaacgcc gtggtgctcg acgtagggtt
     4441 gcccgaccag tcggggcttg cggtgctcga ccagttgaaa cgcgacgtcg cgacgcggca
     4501 tatcccggtc catgtggtct cgggcagcga tcatacccag accgcgctct cgctgggcgc
     4561 gatcggctat gtcatcaagc cggtcgagcg cgaggcgctc gccggggtgc tcgagacgct
     4621 gggcgcgcaa ttggcgcgaa ccgtccgccg tgtcctcatc gtcgaggacg atcccgtcca
     4681 gcgcgaggcc gtcaccaggc ttctcgccgc cggggacgtc gagacggtcg gcgtgggcac
     4741 cgcggccgaa tgcctggagc agctaaagca ggaaaccttc gactgcatgg tgctggacct
     4801 gaccttgccc gatgcctcgg gcttcgagct tctcgagacg ctgcacgagg atgaagcaca
     4861 cggctttccg ccggtgatcg tctacaccgg ccatgaattg tccgccaatg acgaacagca
     4921 tctgcgccgc tattcggact cgatcatcat caagggcgcg cggtcgcccg aacgcctgct
     4981 cgacgaagtc tcgctgttcc tgcaccaggt cgtgaccgaa ctgccgatcg agcagcagaa
     5041 aatgatccag aaggcacgcc accgcgatgc gatcctcgag gggcggcgca ttctcgtcgt
     5101 cgaggacgat gtgcggaatg tctattcgct gaccaatatc ctcgaaccgc gcggggcggt
     5161 gatcgaaatc gctcggaacg ggcgcgaggc gatcgaggcg ctggagaaat ccgcgaatgg
     5221 ggatggcagc gccaccgatc tcgttctgat ggacgtgatg atgccggtga tggacggcct
     5281 gacggccacc cgcgaaatcc gcaaggacgc gcgctggaag aagcttccca tcatcaccct
     5341 gaccgccaag gccatgcccg acgatcagca aaactgcatc gaggcggggg cgagcgatta
     5401 catggcgaag ccgatcgatg tcgacaagct gctgtcgctc gtccgggtct ggatgccgcg
     5461 atagcgccgc catgacccag gagaccgacg atatcgaaat ccagctcctg ctggaggcgc
     5521 tctatcagcg ctatcattat gactttcgca actatgcccg cgcgtcgatc aagcggcgcc
     5581 tgcggcaagc gcgcgagcag atgaacttcg ccaatttcac cgcgatgcag gagagccttc
     5641 tccacgatcc agccatgctg cagcgcttgc tcggttatct cacggtacag gtcagcgaga
     5701 tgttccgcga tgcttcctat ttcagggcca tccgcgaaaa ggtcgtgccg catctgcgca
     5761 cctacccctc gctcaaggtc tggatcgcgg ggtgcagcgc cggcgaggaa ctttattcgc
     5821 tcgtcatcct gtttcgcgaa gaaggccttg aggagcgaac cctcttctat gcgaccgacg
     5881 tcaaccagga cgcgctcgat aaggcgtcaa aggggatcta tgcgctcgat cgcatgaagg
     5941 catttaccga gaatcaccgc gcttcaggcg gcaagtcgtc gctctcggac tattacagca
     6001 ccgcttatgg caacgccgtt ttcgacaaga gtctgcgcga ccgggtggta ttttctgacc
     6061 atagcctcgt caccgacgcg gtgttcgccg agatgcatct ggtttcgtgc cgcaacgtgc
     6121 tgatctactt caaccgtgaa ttgcaggacc gggcgatcgg tctgttccgc gattcgctga
     6181 tccgcaacgg cttcctgggg ctgggctcga aagagaatat gcgcttttcc gcccatgccg
     6241 atgccttcac cgatttcgtg cgcgaagaga aaatctatca aaggcgcgac gcatgagcga
     6301 agtcgcggca cgctgcgtcg ttatcggggc ctccgccggc gcggtgcagg ccttgtcgga
     6361 aatcctgccg gcgcttcccg ccgacttccc gcttcccatt ctggtcgtgg ttcatatccc
     6421 gtcggaccgg cagaatatcc tcccgtcgct tctccaggcg aaatgccgga tgaccgttcg
     6481 cgaggcggaa gacaaggaac cggcgctcgc cggcgtggtc tatttcgctc cgcccgatta
     6541 tcacctgctc gccgaggcgg atggatcgct gtcgctgtcg agcgacgaag aggtgctcta
     6601 ttcccgcccg tcgatcgacg tcttgttcga gagcgcggcg gacgcctatg gcgcggcgct
     6661 gatcggcatc gtcctgaccg gcgcgaaccc cgacggcgca aagggcctca aggccattgc
     6721 cgatgcgggc ggcaccgcgc tggtggaaga ccccgcggcg gcttatgcgg ccgaaatgcc
     6781 gacggcggca ctcaaggaat gcgccgcggc gcgcgtcgtg ccgctcgccg agatggcccc
     6841 caatctgatg aaagaatgcg gcgtatgaac agcaacatgg atccggttca tttcctgctg
     6901 gtcgacgacc tggaagagaa tctcgttgcg ctggaggcgc tcctgaggcg cgacgatctg
     6961 gttcagctca aggcccgctc gggagaggag gctttggagt tgctgctccg tcacgacgtc
     7021 gcactggcct tgctcgatgt gcagatgccg gggatcgacg gtttcgaact ggccgaactc
     7081 ttgcgaggta acgcgcgcac gcggcatatt ccgatcatct tcctcaccgc cggaagcgcc
     7141 gatcggcagc gtcgattccg gggttacgaa gcgggtgcgg tcgatttcat acagaaacct
     7201 ctcgaggcgg atattctgcg tagcaaggcc aatgtatttt tcgacattta tcgccagcgt
     7261 cagcaaatcg ccgcgcagcg tgacgagctc gaaatacaga ctgagacgct gaaagcgttc
     7321 gaacagctga cccgccagca acttgtgcag gagcaggaaa tagcgaggtt ccgtgagcag
     7381 ttcatcgcgg tattgggcca cgatctgcga aacccgctgg cttccatcgg aggcggcgcg
     7441 cgcgtccttc gacgccaatc gctaaacgac aaggcgctca agatcatcga tctgatggag
     7501 gccagcgtgg atcgcatggc cgagttgatc gacaacgtca tggatttcgc gcgcgggaga
     7561 ctgggcggcg gcatcgctct ggatcgccag cccaccgagc tggaccctgt tctgcgacat
     7621 gtcgtcaacg aactccaggc gagcgagccc gaccggaaga tgatgtgcac cttcgcgctt
     7681 cccacgccga tctgcgtcga tcccggccgc atcggccagc tcgcctccaa cctgctcgcc
     7741 aatgccgtgg ttcacggcga ccctcgatcg cctatcgagt tgagggcggt tctggaggac
     7801 ggcgagttca tactttcggt gcgcaatgcg ggcccgaaaa ttcccgaagc cgcgatggaa
     7861 cggctgttcc agccatttgt ccgcggggaa gtccggccca atcagcgcgg gctgggtctg
     7921 gggctgcaca tcgcctcgga gatcgcaaaa gcgcacggcg gaacgctcag cgtaacctcg
     7981 accgaaggcg aaacccgttt taccttcacc atgccagccg agcaggaact ggagacggcc
     8041 gaatagaggc tatcgtccgc gtcggagtgg cgcgggagtt ttgacgatcg attgcctgat
     8101 ttcggatgac tgagagtccg atggcggcgg tagcttgtgt ttttcgagcc ttcctgtggc
     8161 gcactggccg tcgtcgtcct gcttcgctct aaaatagccg gtctgctggc ggccgtcgcg
     8221 cgacctgcct tcaatttaag gcggcgcggc tcagcttctc gcaatcgatg gctgcggcga
     8281 ttgcccccgc gccgtccgca tccttatcct tgcggaggcc gagggccgtt ccgatcgatc
     8341 ggccgatcgt gctcagcagt ccctttccat cgccgccttg agtctttgat ccttgatcga
     8401 agccgatatc gggctgggtc aacgtgccat caatgcgcag cggatcgacg agccgaagcg
     8461 aggccttctt cttggcggtg ccggcgaggg tgagtgcgac cgtttcgccg tcgaggttga
     8521 tctggccgcg gccattgccg cgcgagattg tggtgtcgat ggtgaagggg gccggaacga
     8581 gaacgccgcc ccgggcgctg aaagccatga tcgcgcaccg gatcggtacc atcttttcgt
     8641 cgtcgccgag cttctggccg atggcgccgc cgagatcctg tccgagaacg aaggcggccg
     8701 cccggttcat cgcgccgcca ttggcgacga aggcgatctt gccatttccg gatgcgaagg
     8761 cctgccggat cgtatcgccg cgcccgttga tccggatcag accgtcaatg ggaccgcgta
     8821 tcatgtcggg ttgaccgacg aatgcgtcga aggatgcgcc cctcactcgc agctcggtcg
     8881 aaagcgtcgg cgtgtctttc ctcgaatcga tcgtgaccct ccctgtcatc cggccgcgtt
     8941 ccagatcggc caccgcattt tcgagtttga ggacgcggtg gtcgagggtc agcctgccct
     9001 tgaggctttg gaacaccgaa ccgcccttga ccagcaagcg ggcgatcgaa aagcggatta
     9061 cgccgtcggt cggacccatt ttgctgagat tgatccgcgt gtcgggaatg atgcgcgggc
     9121 cgatgcgtgc ctcctgcgcg cgtgcggcgg cgaggcccgc gtcgtcggca agatcgtcga
     9181 aatcgagttg cggcgcgtcg atggtcgcat cgatcttcgt ccgcccgccg cgtttaagga
     9241 ccgtcgcctt cgcgcgaagg cgcgagcgtc cgatcgtgcc ggtgagcgtg tcgatgaacc
     9301 agtcttcgcc gtgatggcgc acctctcccc taagatcgat atcctgcgtc ccgaacagcc
     9361 ctgcttcgat gatgtaatcg agttgcttga gattcgtgcc gcgggctcgc atttccatgc
     9421 gcatgtcgcg caggttgaga gcgcctgcca tcgtgccttt ggcgtcaagg tcgaggatgg
     9481 cggatgcgag gctggccgaa aagggccaag aggacgttgc gccttttcgg gccggtccgc
     9541 caccgcgcat ggcgacgcga gcaggcgagc cattaaagtc tccgctcgcg tttactcgga
     9601 ggccagcgcg cggctcggcc tcgatcgtcc ccttgaggtc gagccggcgt tttgcgtcgc
     9661 ggagggtgaa gcggccatct ttgatcgtca ggttttcgag ggtggtcgca tcgtttttgc
     9721 cgccctgatc cttgtcggcg cggcctcccc aattgctgtt gccttcaccg tcgcggacga
     9781 ggacgaggtc gaggccttcg acgtcgagcg agcggacatc ggggttgccc gaaagaaggg
     9841 agaagatcgg gacttgcgcg ctgacgcgca ccaccttgag catttcgcct tcgccggccc
     9901 aagccggctg ggcgatctgc aggtcgcgca ccacgatcgt gggcgtgaac gagaagaatt
     9961 cgtcccttga aagcgagcct agcgcaaccg gcgcatgaaa tttggacgat agcctgtctt
    10021 cggcaagccc gcgcagaagg cccaccggga aagcggcgag aagaagcagg gagagaatgg
    10081 cgagcgtcgc agcgatcgcg gctgcgatcc aaagcttgcg ggggagcggg cgccgcacgc
    10141 tagtctgctc gcccatcgcg gtccggtccc ttgcctgtca tcgcatgcct cctgcccgga
    10201 aattcgacgc aaaaaagccc gcacagaaag ctgtgcgggc tgtgccgatt ccgggcaggc
    10261 tcagtagctt gtctcgaatt cgtcgatttc gcgttcggct tcgtcgcggg cgcggccata
    10321 gcgttcctgg attttgccgg cgagaatcct gcgatttccg ttgatctcgt caagctcgtc
    10381 gttggtaagt tcaccccact tcttctgggc gtgaccctta acctgttccc aattgccttt
    10441 cagcatgtcg ctattcatct tctctctcca attgcctgca tggagaagca acgcggcgcc
    10501 atcccggatg ttccgaaaag aagcgaacga gccgccatgg caagttgggc tagaattgcg
    10561 ccctcaatgt tacgtacagc gagcggcccg gctcggggaa gccgtcggtg aggctatatt
    10621 cctcgtcgaa cagattgcgg gcaccgacgc cgagttcgat ctcgtccgtg accgcatagc
    10681 tgatgcggag cgaggcgttc gtgtaggagc cggtgcgata gtaacgcgcg ggctggttcg
    10741 ccggcgttgc cgtgaacaaa gtccagcggc tcgaggcgag ctcgatgctc ggttggatat
    10801 gaaggccttc aagcggggcc cagtcggcgt agatcagacc cttgtgtgtc gggacgcccg
    10861 tgggccggaa gcctgggttc gcggcatagc taaggtcgcg cttgatgtag gtgtagttcg
    10921 cgccgacgtc gaagcccggc gcgagtgtcg cgctgagtga ggcttcggca ccataatagt
    10981 ctccgcttcc gacattctgg ctctgggtga gattgcggag cgtgcagcca ggcaccggcg
    11041 tcgcaggcgg tgtagtcgac gcggtgcagg gataagccgg ggttggtacg ccaaagatcg
    11101 cgtcctcgac ccagctgtag aatatcgccc cgtcgagacg gattgatccc gctgtgaagc
    11161 ttccgccgat ctcggcgttg gttgcgcgtt cgggcctcag cgccgggttg gggatggcgg
    11221 tgttgaagcg ttggctgaag cgctcgaaga tggtcgggaa gcgtgctcgg gaggaaaggc
    11281 tggcgcggag cgtcaggctg tcactggcgt tccagtcgaa gcggccctgc aggttccagg
    11341 tgtcggcatc gcggcgcgga tattgataga ggaccgcagg cagcgtggtg ccgagcggtg
    11401 atccgaactc ttcggcacgc ttgaggtcgc gccagtcgaa actgccgcca agagtgaagc
    11461 ggagcgcgga ggtgagggcg agttcattct cgagcgcgag cgagaagctg ttttcggtct
    11521 gcgtctgcgg cggctcggtc gacgggacgc cggcggtcga gaagctcgtc tgcgcctcga
    11581 catgcttgtc gtgccggtaa tggaaggcga ggcggagcgt gtcggcttcg ctcagcttca
    11641 catcgagctg agccgaaccg ccccatgcct tgtcgaaata ggggctgtcg aacgcgaaag
    11701 gccggctttg cgttgtctgc gtgcggtcat tgaacgagcg caggatcgag tcgaactggt
    11761 tgtaataggc gcgcgtcttc agcgtgccgc gatcgccgag cgcggtggtc gacaggaaat
    11821 agacgctttc gatgtcccac ttgggccagt tccagaagcg cggtgcggag accgtgtcgc
    11881 ttatgtgaag cggggcattt tttgcgccag cctgcttcgt atagctgatc gcatattcgt
    11941 cggtgtcatt gggggtgaag ccgagcttga tgttcgcgcg ccagtcgcgc gtgcgcgaaa
    12001 agtcccgttc gccgccgtcc tcgatcgtgg gaacgacggg tgtgaagttg cccggcagat
    12061 cccaatggtc ggtgaaactg cgcgcatagc tggcttgcgc ataccatctg tcctgccgcg
    12121 tcccgatcat ccccgaagcg gaatagccgg cataatcggc atcgcggtcg aaattgacgg
    12181 tgccgcgcac atcgatgtcg agcgccttgg tcgggcgccg tgtcacgagg ttgatcgcgc
    12241 cgcccatcgc gccgggtccg tcgagcaccg aggcatagcc cttggcgact tgcacctcgg
    12301 cgatgtcggt ggtgaggaag cggccatagt cgagacggtt gtcggcgggc agatagacgc
    12361 ggatgccgtc gatcgagagc ggcacctgaa agcggtcgaa gccgcgcacg aagacgaggc
    12421 gctcgttgcg cgtgccgccg ctgttgccgg cgacgacgcc cggcatcagg ttaacggcat
    12481 cgtcgagcgc ggtgcggttg aacttgtaga tcgcctccga cgacagcgtc tcgccgtcga
    12541 tcccgatccc ttcgctccgc ggggcggtga cgacaatctg gccgagcgcg aagcggtcat
    12601 cctctgccgc cggtgctgtc ccgtcctgtg cgtgggctgc ggtggagata aggcaggcgg
    12661 tcgtgatcag gagagcaata gcggtgcgca tgattttccc acttttgtat attatcgatt
    12721 cgctatatcg agccgtatat agttgtttcg atcaaaagga agcgccagtt gctcagatta
    12781 ttgctcgttt ccggggtagc gccgctcttc cttgtcgcgg gtgccgcagc gtcgccccct
    12841 gtcccggagg ccgcctgcac cgcacccgga gagctgcccg ccgcactggc cagctgggct
    12901 tcgcctgcct ttctcaaagc ggcgccgaac tataaatcct cgcggcatag cccgttgata
    12961 cccgggaagg ccgcagtaat tagtcttctg cagacgccga aggtcgacta tccggttcgt
    13021 cccgaaaagc cgggcggtac ggtgagtttc ggcggggtcc tacgcttcac ggtacccgac
    13081 gagggcgtgt ggagagtggc tctgagcaca gcggcgtggg tcgatgtcgt caaggacggg
    13141 caggcatcga cgtcgatcgc gcacgggcgc ggacccgcgt gcaccggaat ccgcaagatg
    13201 gtcgattacc gccttgcccc cggcacttac gcgctgcaga tcgccgccaa cggctccgag
    13261 acggtgacgc tcatggtcgc gcacctgccg tgaggctttt gcgggctgcc gcggcgctgc
    13321 tgctggtggc ggcaacgggg ccggcctgga catggaactt gccggaaggc gtcggcgcgc
    13381 ccgcaatccc tgcgggcaat gccatgggcg cggcaaaggt cgagcttggg cggcggctct
    13441 tctacgacgc cgacctgtcg gctgatggga cgctcgcctg tgccggctgc catgagcaaa
    13501 agcgagcgtt cgccgacggg aacgcaaccc gtcccggcgt ggatggcagt ccgggacggc
    13561 gcaacgcgcc gggcctcgcc aacattgcct ggttcgatcg cctgaccttt gccgatccgg
    13621 ccgcgaccac gctcgagcgg caggcgctga tcccgctctt cggcgagcat ccggtcgaga
    13681 tgggcatgaa gggcagggag gccgagttcg agcggcgtct cggtgctgat cgctgctacc
    13741 gacggatgtt tcgaaatgcc tttcccgaga agcggggtaa gatcgatgtt gaaagcgtca
    13801 cggctgcgct cgctgccttc cagcggacga tgacctccta tcgcagcccc tatgacctgt
    13861 atcgtgcggg cgaggccggt gctttgccgg agaccgcgca gcgaggcgaa cgcctgtttc
    13921 gagcccgcgg ctgcaccgcc tgtcatgccg ggccgaacct cagcgatatg cgctatcatc
    13981 gactggagcc ggcgcgagcg gccgaccccg gactgatcga gaagaccggc cagcccgacg
    14041 atgcagggcg tttccggacg ccatcgctcc gcaacgctgc ggtcactggg ccatggtggc
    14101 acgacggcag cgaacggaac ctcgatggcg cgatctctcg tcatcccaac cctgtgtccg
    14161 ccgaggaggc gtccgcgata ggagcgtttc tgagcgcgct caccgatccg cacttcatcg
    14221 ccgacccacg tctctcacgt cccgatacgg cgtgcggcaa gccgctatga agtgggtgtc
    14281 ccgtcgaccg gcatcggctc tgcgagaagg agcgcgtcga gctcctggaa cataataggg
    14341 gacgcggccc tgcccgtggc tttctgcatc gagcggtagg cagacaatat tgcttcgccg
    14401 acgtctgtaa cgcgcgcgcc gcgggtctgg ccgccgccgg gaacggtgtc gacgagacgg
    14461 tctttccagc acctgttcat ggcatcgacg agtagccagg cccgccgata gctcatgccg
    14521 agcgcgcggc ccgccgcaga gatcgatcca tgctcgcgga tggcgtcaag gaggtcggcc
    14581 ttgccggggc ccatggcgat ctcgtcgccg caataaatct ggatcttgat tttcaggggc
    14641 ggggtcggtg ccatcgacag aggatagccg atcggtcgcc gaggtaaatt cgattcatga
    14701 ttgcgcccgt tttccacgct gtcggagaaa cgctgacggc ctgcaaccgg ccgatagccg
    14761 ccatccgcga tctaagccag ccgcggcatc gtccccgctg cggcgcctgc agcaggctcc
    14821 gccggcagga acgtcagcgc cttcacgaac tgcgcggtgc aattgggcca gccataacgt
    14881 gcgccgagcg ccgccgcgtc gccccggtca catagcagcg ctgcgtcgat cgcgcggccc
    14941 agatcctcgt cgagcgcgcc ggctccctcg cggatgatgt cgccggggcc gggcactgga
    15001 taggcggcga cgggcgtgcc gcagctcagc gcctcgatga cgaccagccc gaaggtgtcg
    15061 gtgcggctcg gaaagacgaa gacgtcggcg ccggcatagg ctgccgcgag cgcttcgccg
    15121 cccagcttgc ccaggaacag agcattcggg tgccgcgcct tcagttcggc gagcgcgggg
    15181 ccttcgccga cgacgacctt ggagcccggg cggtcgctgt cgagaaaggc gccgatattc
    15241 ttttcgaccg cgacgcggcc gacatagagc tggatcggcc gcgccaaccc ggcataggcc
    15301 ggatgcggcg tgcggcaggg gcggaacagc gcatgatcga caccgcgttc ccacatcatc
    15361 gtccgggtca ggccctgccc ggcgagttcg cgcgccaggc tgcgcgtcgc gaccatgata
    15421 tgctgcgcgg ggcggtggaa ccagcggatg aagcgccaga cgagcgccgg cgagacgggc
    15481 aggcgcgccg cgacatattc gggaaaacgc gtgtgatagg cggtggtgaa gggaaaatcg
    15541 ttctgcaggc accagcgccg cgcggcgagg ccgagcggtc cttcggtcga aatatggatc
    15601 gcctgcgggg cgaaggcgcg aatgcggcgc ccgatggcgc gccggccggc gaaggcgagg
    15661 cggatctcgg cataggaggg gcaggggatc gaacggaaga ggtcgggcga gatgatgccg
    15721 acctcatgcc ccgccgcgcg cagctcggcg atcgtggctt gcagcgtgcg gacgacgccg
    15781 ttgacctgcg gctcccacgc gtcggtgacg atcaatatcc tcatgcggcg gccggcagcg
    15841 cgagcttcgt cggcgcgctc cgcgccgcga cctcctcggc ccagtggagg atttccatgc
    15901 gtccgtcatg atgctcgacg agtgcggtgc agccttcgac ccagtcgccg tcgttataat
    15961 attcggtgcc ctcgatctcg cgcatctcag cgctgtggat gtgaccgcac acgacgccat
    16021 cgaccccgcg cgagcgcgcc gcgtgcgcga cgacttcctc atagcggccg atgaactgga
    16081 ccgcgttctt gaccttgtgc ttggcgacca tcgacagcga ccaatagggc aggccgaagg
    16141 cgctgcggat gcggttcacg accacattgc acttcatcag cgcggtgtag gcggcgtcgc
    16201 ccacgaaggc gagccagcgg tgcgcgagca tcaccgcgtc gaactcgtcg ccatggacga
    16261 cgagcaattt gcggccatcg gcggtttcgt ggatcgcctc gcggcggatc tcgaccccgc
    16321 cgaaatcgaa gccgtcgaag ggcttgcaca tctcgtcatg gttgccgggc acatagacga
    16381 cgcgtgtgcc gcgcttcgcg cgcttgagca cgcgccagac gacgtcgtta tgttcgggcg
    16441 gccagaagtg gcgcttcttg aggcgccaac cgtcgatgat gtcgccgacg agatagagcg
    16501 tctcgcaatc gacatggtcg aagaagtcga tcagcatcgc ggcgttgcac ccccgagtgc
    16561 cgaggtggat gtcgctgacc catatggtgc gatagctccg ccgctcgccg atcacgcgtt
    16621 cgggaatatg cgggtcggtc gtgaacgggt cgggaaaccg ggcggggatc ggcagggtcg
    16681 aaatcgaggc catgtcgtgc tccgtaaaca gcttttggct gcccacggcc tagacaggag
    16741 ttttgttaca gcgggcgatt caaactcgcg acagtttgaa gaaagtttga aggcgctttg
    16801 gtgacgttgc ctatttgaca catatgcggt taaccatgat ccaatcaggt tcgccggatc
    16861 accaaattcc tgatttcgct catatcttcc atcgcgaagc ggatcccctc gcggcccagc
    16921 ccgctgtcct tgaccccgcc atagggcata ttgtcgacgc ggtaggagga gacgtcgttg
    16981 accacgatgc cgccgacgtc gagatgatcc cacgcctcca gcaccttgtg caggtcgcgg
    17041 gtgaaaaggc ccgcttgcag cccgaacttg ctgtcgttga tctcggcgag cgcggcgtcc
    17101 cagtcggtga agctcgacaa tatggcgagc gggccgaagg cttcctcgcg ataggcgtcg
    17161 gcgccgcggt cgacgtcttc gagcaaggtc gcctgcagca tcgcgccgtc gcgcttgccg
    17221 ccgacgagca gtttcgcgcc gcccgcgacc gcggcctgaa tccagtcgtc gagccgcgcg
    17281 gcttcctttt cggagatcat cggaccgatg aaggtgtcgc ggctcttggg gtcgcccgcg
    17341 accagcttct tggtctttgc gacgagcatg tcgcggaact tgtcgtagat atcggcatgg
    17401 atgatgatcc gctgcacgcc gatgcacgac tggcccgatt gatagaaggc gccgaagatg
    17461 atccgcgcga gcgcatggtc gaggtcggcg tccttgtcga cgatcaccgc ggcgttaccg
    17521 ccaagttcga gcacgacctt cttcttcccc gccttggcct tcaaatccca gccgacgccg
    17581 ggcgagccgg tgaagctcag cagtttcagc cgctcgtcgg tggtgaagag gtcggcgccc
    17641 tcgcgtgtcg cgggcaggat gctgaacgcg ccctcgggca agatgtcgca ttcggcaagc
    17701 acctcgccca tgatgatcgc gcccaatggc gtcagcgacg cgggcttcat cacgaagggg
    17761 caacccatcg cgatcgccgg cgcgatcttg tgcgcggcga ggttgagcgg gaaattgaac
    17821 ggcgagatga agctgcacgg gccgatcggc acgcgcttcc acatccccat atagccctta
    17881 gcgcgcgccg agatgtcgag cggctggacc tcgccatagt tgcgcgtcgc ttcctcggcg
    17941 gcgatgcgga aggtgtcgat caggcgcgtg acctcgccct ctgaatcggc gatcggcttg
    18001 cccgcctcga cgcacagcgc ataggcgagc tcgtcaaacc gttcctgaaa ccgcccgacg
    18061 cagtgggtca gcacgtcgcg cttctcgaaa ctcgcgagcc gcgccatcgg ctcggccgcg
    18121 cgcaccgcgc ccgcgatggc ttcgtcgatg acgtcgggtg tcgcaagcgc ggtgcggaac
    18181 gcgacctcgc cggtgaactt gtcggtcacc gcgaggtcgg tgttcggctg gaccgccttg
    18241 ttgttgagat agagcgggta gacgtccttg agcttcattt cctactccgt tcgggctgag
    18301 cctgtcgaag ccctgtcctt ctttgaagac aaaaaaacgg cccttcgaca agctcagggc
    18361 gaacgggttt gggaccctca aagttccttc gacaacctct tgatgtcgtt gttcaaaatc
    18421 tgatcattct ccgaataatc gaccggacag tcgatcaggt gcacccccgg agtatcgcgc
    18481 gtgtgggcga gaagttcctt cagatgcgcg gcgctttcga cgcggtgccc gtgcgcgcca
    18541 taagcctccg catatttcac gaaatcgggg ttgccatagg tcagccccca atccttgaag
    18601 cccatgttgg cctgtttcca gcggatcatg ccatagctgt tgtcgttcaa aatcagcacg
    18661 gtgatgttga gcccgaggcg caccgcggtc tccatctcct ggctgttcat catgaaaccg
    18721 ccgtcgccgc agatcgccat gaccttgcgg tccggataga gcatcgcgga catcatcgcc
    18781 gacggcagcc cggcgcccat cgtcgcgagc gcattgtcga gcagcacggt gttcggcttg
    18841 cacgcggtgt agccgcgcgc gaaccagatc ttgtagacgc cattgtcgag gcagatgatc
    18901 ccgtcgtcgg gcatcgcgtc gcggaccgac tgcaccagat gcggcgggaa gatcgggaag
    18961 cgctcgtcct tcgcgagcga ttccgtatgc tcgacctcgg ccttgcgata ggcgagcaga
    19021 tggtcgaatt tccagccgcc gttggggacg atatcctctt ttatctgcca caccgcattg
    19081 gcgatgtcgc cgatcacctc gatgtcgggg aaatagaccg gatcgacctc tgcggtcttg
    19141 gtcgacacat ggatcaccgt cgggccgccg cgctgcatga agaagggcgg cttttcgatc
    19201 acatcgtggc cgaggttgac gatgcaatcc gatgcctcga ccgcgcggtg gacgaaatcg
    19261 cccgacgaca gcgccgcgca gccgaggaat ttcggatgcc gctcatcgat cacacccttg
    19321 ccgagctggg tcgtcaggaa ggggatgccg gtcttttcga tgaattgcag cagcatgcag
    19381 ctcgtcatcg tccggttcgc gcccgcgccg atcaccagca ccggcgactt cgccttttcg
    19441 agcgccttta ccgcctcgcg gatcgatttg ctatcggcgt tgggacggcg cgaatggctg
    19501 cgcttcagcg gcgtcgaatc ggtatgttcg tcggcgatat cctcaggcag ctcgatatgc
    19561 accgcgccgg gtttttcctc ttcggcgagg cgaaaggctt cgcgcacgcg gctcggaata
    19621 ttgtccgagg atgccatctg gtgcgtatat ttggtgatcg gtcccatcat cgcgacgacg
    19681 tcgagaatct gaaagcggcc ctgtttcgac ttcttgatcg gcttttgcca ggtgatcatc
    19741 atcatcggca tgccgccgag ctgggcgtat gcggccgcgg tgacgaagtt cgtcgcgccg
    19801 gggccgagcg tcgcgaggca gacgccggtc ttgccggtgt ggcgcccgta agtcgccgcc
    19861 ataaagcccg cgccctgctc gtgccgcgtc aggatcagct tgatcttctt cgaccgcgac
    19921 aggctgtcga gaaaatcgag attctcctcg cccggaacgc cgaaaatata ctcgacgcct
    19981 tcttcctcca ggcaatcgat gaacaggtcg gatgcttttt tcatgctaca gtcgccccct
    20041 cagccacgca tcgtcatgat gcggccccaa gtcatgccgg cgacccctca gcactatgtc
    20101 gttgcaacga ggctcgtctc aggccgcgag tccctgctgc atcgggaagg gtttgtaacg
    20161 aggggcaaag gggttgtcac ccacccgcgc cgaagtgtcc cggtcagcgt ctttcgatca
    20221 gaagcgatcg atcgaggcgc gcggaaacgg ccgatcccgc aggtatctcg gcgctgcgcc
    20281 ctgtggtgac aaagggaagg acgaag
//
LOCUS       DATMSU010000372         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_177040,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000372 DATMSU010000000
VERSION     DATMSU010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177040"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1173)
                     /locus_tag="VN034_10000"
     CDS             complement(82..1173)
                     /locus_tag="VN034_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HWV60978.1"
                     /translation="MFTLFALLLAGLVFAAPAHAERIKDMGQFQGLRANQLTGYGLVV
                     GLAGTGDDSLDYSTLGMRGAVSRFGLTLPPGVNPALKNAAAVMITAELPPFAKPGQRL
                     DVTVSAIGKAKSLRGGTLVLAPLYGADGQIYAMVQGNLVIGGLGVDAADGSKLTVNVP
                     SSGRIAGGATVERAVDTGFATASYLTFNLHQFDATNAKRVTDAINAAIPNTASMIDGA
                     SIAIRAGGNGDERMRLMSKIENLGVTRAEPPAKVIVNARTGTVVINGAVRVGTAAVSH
                     GKLTVSIKEAPMVVQPAPFSRGQTAIEPSSEIEVTEDYRPAFLMNPNASLSDLVDSIN
                     RLGVPPGDLVAILEALSQAGALTAELVII"
     gene            complement(1205..1864)
                     /locus_tag="VN034_10005"
     CDS             complement(1205..1864)
                     /locus_tag="VN034_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HWV60979.1"
                     /translation="MRRRLSKLAMLALLLAPVTAQAKDKLPPDAFAASMPLPPPPPPA
                     NGSIFQGAYVPLTSGGRAGAVGDIITIQLVERTAATKSNAAGTQRDGDIGLSPPTTGP
                     LSLFNPSDIGMGGGQQFKGKGEASQSNALSGEVSVTVAAVYPNGTMLVKGEKFLTLNR
                     GDERVQIAGIVRAIDISPDNRVLSTRVANAHIRYVGKGEIARASQQGWLQRFFSIISP
                     F"
     gene            complement(1845..>2028)
                     /locus_tag="VN034_10010"
     CDS             complement(1845..>2028)
                     /locus_tag="VN034_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HWV60980.1"
                     /translation="LVGGAGEEGRGSLRGGMLEGSNVNVVEELVDMIETQRAYEVNSK
                     MISATDEMMKNASQTL"
BASE COUNT          312 a          674 c          697 g          345 t
ORIGIN      
        1 cggccgcctt gcgcagcccg ccgtcgccgc cgcccgcagc cgcgggggtg ggcgtcgccg
       61 aaacggaaga aatggggctc gtcatatgat caccagttcc gcagtgagcg cgccggcctg
      121 gctcagcgcc tcgaggatgg cgacgaggtc gccgggggga acgcccagcc ggttgatcga
      181 atcgaccaga tcggagagcg aggcattggg gttcatcagg aaagccgggc gataatcttc
      241 ggtgacttcg atctcgctcg acggttcgat cgcggtctgg ccgcggctga acggcgccgg
      301 ctggacaacc atcggcgctt ccttgatcga cacggtgagc ttgccgtggc tgaccgcggc
      361 ggtgccgaca cggaccgcgc cgttgatcac cacggtgccg gtgcgcgcgt tgacgatcac
      421 cttcgccggc ggttcggcgc gcgtcacgcc gagattctcg atcttcgaca tcaaccgcat
      481 ccgctcgtcg ccgttgccgc cggcgcggat cgcgatgctc gcgccgtcga tcatcgaggc
      541 ggtattcggg atcgcggcgt tgatcgcgtc ggtgacgcgc ttggcattgg tcgcgtcgaa
      601 ctggtggagg ttgaaggtca gatagcttgc ggtcgcaaag ccggtgtcga ccgcgcgttc
      661 gaccgtcgcg ccgcccgcga tgcgtccgct cgacggcaca ttgaccgtca gcttcgaccc
      721 gtcggcggcg tcgacgccga gcccgccgat caccagattg ccctgcacca tcgcgtaaat
      781 ctgtccgtcg gcaccataga gcggcgcgag cacgagggtg ccgccgcgca ggctcttcgc
      841 cttgccgatc gccgacacgg tgacgtcgag gcgctggccg ggcttggcga agggcggcag
      901 ctcggcggtg atcatcaccg ccgctgcgtt cttgagcgcc gggttcaccc ccggcggcaa
      961 tgtcaggccg aaacgcgaca ccgcgccccg catgcccagc gtcgaataat cgaggctgtc
     1021 gtcgccggtg cccgcgaggc ccacgaccag gccgtagccc gtcagctggt tcgcacgcag
     1081 cccctggaac tggcccatgt ccttgatgcg ctcggcgtgc gcgggagccg cgaagacgag
     1141 gcccgcgagg agcagcgcga aaagggtgaa cagggtcgga cgctgggtca ctttaaattc
     1201 cttgttagaa ggggctgatg atcgagaaga agcgctgcag ccaaccttgc tggctcgcgc
     1261 gggcgatctc gcccttgccg acgtagcgga tgtgcgcatt ggcgacgcgc gtcgacagga
     1321 cgcggttgtc gggactaatg tcgatcgcgc ggacgatgcc ggcgatctgg acgcgctcgt
     1381 cgccgcggtt gagggtcagg aatttctcgc ccttcaccag catcgtaccg ttgggataga
     1441 cggcggcgac ggtcacgctg acctcgcccg acagcgcgtt cgactgcgac gcttcgccct
     1501 tgcccttgaa ctgctggccg ccgcccatgc cgatgtcgct ggggttgaac agcgacagcg
     1561 ggccggtggt cggcgggctc aggccgatat cgccgtcgcg ctgggtgcct gcggcgttgc
     1621 tcttcgtcgc ggcggtgcgc tcgacgagct ggatcgtgat gatgtcgccg accgcgcccg
     1681 cgcgcccgcc cgacgtcagc ggcacataag cgccctgaaa gatcgagccg ttcgccggtg
     1741 gcggcggagg cggcagcggc atcgacgccg caaaggcgtc gggcggcagc ttgtccttcg
     1801 cctgcgccgt gacaggcgca agcaagagcg cgagcatcgc cagcttagag agtctgcgac
     1861 gcatttttca tcatctcgtc ggtcgccgaa atcatcttcg aattgacttc ataggcgcgc
     1921 tgcgtctcga tcatgtcgac gagttcctcg acgacattga cgttcgatcc ctcgagcatc
     1981 ccgccgcgaa gcgacccgcg gccttcctcg cccgcgccgc cgacgagc
//
LOCUS       DATMSU010000373         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_120494,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000373 DATMSU010000000
VERSION     DATMSU010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120494"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VN034_10015"
     CDS             <1..238
                     /locus_tag="VN034_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HWV60981.1"
                     /translation="TQVAGEGAAEVEAGALRGDVDGAMARLPDEQREVVMLILVEGYA
                     YREAAEMLDVPVGTVTSRLARGREALMFYLGEAA"
     gene            235..951
                     /locus_tag="VN034_10020"
     CDS             235..951
                     /locus_tag="VN034_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWV60982.1"
                     /translation="MSFDPATVAAFVDGELDDLTARRIEREAATDAALAAEIARHRTL
                     KAQLMAHYTPIATEAVPERLRSLLTVNDAADDRVDTSLADRRIARHARFSGMHWGAIA
                     ASLILGLTIGLRPWVPAADVAIDSGTLVAAGPLAEALDTQLASTQPGDAAVRIVLSFE
                     DKAGRYCRSFESRAIDGIGCREQDRWQIERTLPGQTRGDYRQASSGELAAAAAAMMKR
                     DALDAGDERAARDAGWVRHR"
     gene            1162..1935
                     /gene="mazG"
                     /locus_tag="VN034_10025"
     CDS             1162..1935
                     /gene="mazG"
                     /locus_tag="VN034_10025"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HWV60983.1"
                     /translation="MPDAANQSPIDRLLLIMRQLRNPDGGCEWDLAQDFSTIAPYTIE
                     EAYEVSDAIAGGDPAAIRDELGDLLLQVVFHSQIATDDGLFGFDDVANAISDKMERRH
                     PHIFGDAKTDDVRRQWEAIKADERAAEGPSGALAGVALSLPALLRAQKLQGRAARVGF
                     DWPDTDGPRDKIAEELAEVTGASDDKERHEEVGDLLFAVVNYARKLGVDAEGALRDAN
                     AKFARRFAAMEDRAGGTLTGLSLDAQEAHWQAVKLSERP"
     gene            complement(1913..>3146)
                     /gene="hflX"
                     /locus_tag="VN034_10030"
     CDS             complement(1913..>3146)
                     /gene="hflX"
                     /locus_tag="VN034_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWV60984.1"
                     /translation="RGLALAIGLDVVAVHTLRLRQTRAATLLGVGQIDAIAPDIAAKG
                     VQLVVVDAALSPIQQRNLETAFGTKVIDRTGLILEIFGERAATAEGRLQVELAHLDYQ
                     AGRLVRSWTHLERQRGGFGFLGGPGETQIEADRRMIRNRMARIRRSLEDARRTRQLQR
                     SKRQRAPWPVIALVGYTNAGKSTFFNRLTGSDVMAEDMLFATLDPTMREIRLPGIDKA
                     ILSDTVGFVSDLPTELVAAFRATLEEVTTADLIVHVRDIVHPDSEAQYDDVRAILNSL
                     GANGPQDGGEGDAAEAIPQIEIWNKVDTADADRRAIIEEMAARRPDVAVISAVTGEGV
                     EAARILMASQLTARHQLQRIHLGYEQGEAMAWLHARGEVVADEPEGEGHVLTVRLDPA
                     DRARFERLWPNLRDAPTA"
BASE COUNT          534 a         1035 c         1044 g          533 t
ORIGIN      
        1 gacgcaagtc gcgggcgaag gcgccgccga ggtcgaggcg ggtgcgctgc gcggcgatgt
       61 cgacggcgcc atggcccggc ttcccgacga acagcgcgaa gtggtgatgc tcatactcgt
      121 cgaaggctat gcctatcgcg aagccgcgga gatgctcgat gtgccggtcg gcaccgtcac
      181 ttcgcgcctc gcgcgcgggc gcgaggcatt gatgttctat ctcggagaag ccgcatgagc
      241 tttgatcccg ctaccgtcgc cgctttcgtc gacggcgaac tcgacgacct gacagcgcgc
      301 cggatcgagc gcgaggccgc caccgacgcg gcgctcgcgg ccgagatcgc gcggcaccgc
      361 acgctcaagg cgcagctgat ggcgcattat actcccatcg cgacagaagc ggttcccgaa
      421 cggctccggt cgctgctcac ggtcaatgac gcagccgatg acagggtcga taccagtctg
      481 gccgatcgcc gcatcgcccg gcacgcgcgc ttcagcggca tgcattgggg cgccatcgcg
      541 gcctcgctca tactcggcct cacgatcgga ctgcgcccat gggtgcctgc tgcggatgtg
      601 gcgatcgaca gcggcacgct ggttgcggcg ggcccgctcg ccgaggccct cgatacgcag
      661 ctcgcctcaa cccagcccgg cgatgccgca gtgcggatcg tcctcagctt cgaggacaag
      721 gccggccgct attgccgcag cttcgaaagc cgcgcgatcg acggcatcgg gtgccgcgag
      781 caggatcgct ggcagatcga aaggacgctg ccgggccaga cgcgcggcga ttaccggcag
      841 gcatcatcgg gcgaacttgc cgcggcggcc gcggccatga tgaagcgtga cgcgctcgat
      901 gctggtgatg aacgggcggc gcgggacgcc ggatgggtcc ggcatcgata gagctgtttg
      961 gttggacctc gtcatcccgg cgaaggccgg gatctcgccg gtgcgttgac ctgacacgat
     1021 gatatcccgg ccttcgccgg gatgacgaaa tatggagggc agtgtttcag tctagtccga
     1081 tcacgctata gagtggccgg cgctgtcccc agatggccca gctgaattta tttaacataa
     1141 tatatattat cggataatca tatgcccgac gcggcgaacc aatcccccat cgaccgccta
     1201 ctcctcatca tgcgccagtt gcgcaatccc gacggcggtt gcgaatggga tctcgcgcag
     1261 gatttttcga cgatcgcgcc ctatacgatc gaggaagcct atgaggtttc cgacgcgatc
     1321 gccggcggcg atcccgctgc gatccgcgac gaactcggcg accttctgct tcaggtcgtg
     1381 ttccacagcc agatcgcgac cgacgacggc cttttcgggt tcgacgatgt cgccaacgcg
     1441 atcagcgaca agatggagcg ccgccatccg cacatcttcg gcgatgccaa gaccgacgat
     1501 gtccgccggc aatgggaagc gatcaaggcc gacgagcgcg ccgccgaagg gccgagcggc
     1561 gcattggcgg gcgtcgcgct gtcgctaccg gccttgctcc gcgcccagaa actgcaaggc
     1621 cgcgccgctc gcgtcggttt cgattggccc gataccgacg gaccgcgcga caagatcgcc
     1681 gaagaattgg ccgaagtcac aggtgcaagt gatgacaagg agcgtcacga ggaagtgggt
     1741 gacctgttgt ttgccgtcgt caactacgca cgcaagctgg gtgtcgatgc cgaaggcgcg
     1801 ttgcgcgatg ccaatgccaa gttcgcgcgc cgcttcgcgg ccatggagga ccgcgcgggc
     1861 ggaacgctga cgggcctgtc gctcgacgca caggaagcgc attggcaggc ggttaagctg
     1921 tcggagcgtc cctgaggttc ggccacagtc gttcgaagcg cgcccggtcg gcgggatcga
     1981 gccggacggt cagcacatgc ccctcaccct ccggttcgtc ggcgaccact tcgccgcgcg
     2041 catggagcca cgccatcgct tcgccctgct catagcccag atggatgcgt tgtagctgat
     2101 gccgcgcggt caattgtgac gccatgagga tgcgcgcggc ctcgacgccc tcgcccgtca
     2161 ccgccgaaat gaccgcgacg tcgggacgcc gcgccgccat ctcttcgata atcgcgcggc
     2221 gatcggcatc ggcggtgtcg accttgttcc atatctcgat ctgcgggatc gcctcggccg
     2281 catcgccctc cccgccatcc tgcgggccgt tcgcgccgag cgaattgagg atcgcgcgca
     2341 catcgtcata ttgcgcttcg ctgtcgggat ggacgatgtc gcgcacatgg acgatcagat
     2401 cggcggtggt gacctcttcc agcgtcgccc ggaacgcggc gacgagttcg gtcggcaagt
     2461 cggacacgaa accgaccgtg tccgacagga tcgccttgtc gatcccgggc agccggattt
     2521 cgcgcatcgt cggatcgagc gtggcgaaca gcatatcttc cgccatgacg tcacttcccg
     2581 tcaacctgtt gaagaaggtt gattttccgg cgttggtata gccgacgagc gcgatgacgg
     2641 gccatggtgc acgctggcgt ttcgaccgtt ggagctggcg cgtacgccgc gcatcctcga
     2701 ggctgcgccg gatccgcgcc atccggttgc ggatcatccg gcggtcggcc tcgatctgcg
     2761 tttcaccggg gccgccgagg aagccgaaac cgccgcgctg gcgctcgagg tgggtccaac
     2821 tgcgcacgag ccgccccgcc tgatagtcga gatgcgcgag ttcgacctgc aagcggccct
     2881 cggccgtcgc cgcgcgctcg ccgaagattt cgaggatcaa gccggtgcgg tcgatcacct
     2941 tcgtaccgaa ggcggtttcg agattgcgct gctgaatcgg gctgagcgca gcatcgacga
     3001 ccacaagctg gacgcccttt gccgctatgt cgggcgcgat cgcgtcgatc tgcccgacgc
     3061 cgagcagggt cgcggcgcgc gtctggcgca ggcgcagcgt gtggacggcg acgacatcga
     3121 gcccgatcgc gagcgcaagc ccccgc
//
LOCUS       DATMSU010000374         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_91970, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000374 DATMSU010000000
VERSION     DATMSU010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91970"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VN034_10035"
     CDS             <1..942
                     /locus_tag="VN034_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076922.1"
                     /note="type IV secretion system VirB4 family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HWV60985.1"
                     /translation="AGLAFQPLATIDRPDDRAWAATWIAALFAHENVPVTPELKEALW
                     SALTSLATAPAGERTLTGLSLLLQSAPLRTALAPYTLEGPFGRLLDAAEDGLAFADVQ
                     CFETEALMGQAGVAAPLLTYLFHRLEERFDGRPTLLILDEAWIFLDHPLFAARIREWL
                     KVLRKKNVSVVFATQSLADIADSPIAPAIIESCPQRILLPNDRAIEPQSEAAYARFGL
                     NARQIELIARATPKRQYYLQSARGNRLFELGLGPVALALCGASDPDTHKRIDGILAEQ
                     GGADFAAHFLRGAGLHWAAELLAGFPGPLAPEEGEIA"
     gene            939..1658
                     /gene="trbJ"
                     /locus_tag="VN034_10040"
     CDS             939..1658
                     /gene="trbJ"
                     /locus_tag="VN034_10040"
                     /inference="COORDINATES: protein motif:HMM:NF010448.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HWV60986.1"
                     /translation="MKTMILGLAAMSFAPLAIGFAPPAHAVPVFDSANYSQNVLTAAR
                     TLQQINQQIRSLQNEAQMLVNQQKNLSRVDFPQMSALQQRLAEIDRLMGQAQGIEFRL
                     DRLDGQFRAAFPTSFEHLARRDQRLATARARIEAEMESFRHAMSVQSGIVENVRSDTE
                     TLRAIVERSQGAEGGLQAAQAANQLLALAAKQQFQIQQLMAAQFREQTFERAKRAQIE
                     REARESTRRFLGDGKAWTPPR"
     gene            1808..>2674
                     /gene="trbL"
                     /locus_tag="VN034_10045"
     CDS             1808..>2674
                     /gene="trbL"
                     /locus_tag="VN034_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HWV60987.1"
                     /translation="MDDLNIIDQFMAAFVAYIDSGFGLLGPDVGFLTTALIGIDITLA
                     GLFWALGGEDDVIAKFLKKILYVGAFALILNNFQSLSDIIFRSFAGLGLTAGGSAIGA
                     SDLLLPGRLGGTGFEAASPLLESAKELVGPIAFFENFVEIAVLLFAWLIVIAAFFILA
                     VQMFITILEFKLTTLAGFVLVPFALWNRTAFLAERVLGSVITSGIKVMVLAVIVGIGS
                     NYFADFTSALAGEEVTIEQAVSLMLASLALLGLSIFGPAIASGLVAGAPQLGAGSIAA
                     TSAFAAGGVVMAG"
BASE COUNT          436 a          919 c          849 g          470 t
ORIGIN      
        1 gccggccttg ccttccagcc gctcgcgaca atcgaccgcc ctgacgaccg cgcctgggcc
       61 gcgacatgga tcgcggcgct cttcgcgcat gaaaatgtgc cggtcacccc cgagctcaag
      121 gaagccttgt ggtcggcgct caccagcctc gcgaccgcgc ccgccggcga gcggacactg
      181 accggcctgt cgctgctgct ccagtccgcg ccgctgcgaa ccgcgcttgc gccttatacg
      241 ctcgaaggtc cgttcgggcg gcttctcgac gccgccgagg acggtctcgc cttcgccgac
      301 gtccaatgtt tcgagaccga ggcgttgatg ggccaggcag gggtcgccgc gccgctgctc
      361 acctatctct tccaccggct cgaggaacgc ttcgacgggc ggccgacgct gctcattctc
      421 gacgaggctt ggatattcct cgaccatccg ctcttcgctg cgcgcattcg cgaatggctc
      481 aaggtgctgc gcaagaagaa tgtctcggtc gtcttcgcga cgcaatcgct cgccgatatc
      541 gccgacagtc cgatcgcgcc cgcgatcatc gaaagctgcc cgcagcgcat cttgctgccc
      601 aatgaccggg ccatcgagcc gcagtcggaa gcggcctatg cgcgcttcgg gctcaatgcc
      661 cggcagatcg agctgatcgc gcgcgcgacg ccgaagcggc aatattatct gcaatcggcg
      721 cgcggcaacc ggctgttcga gctcgggctc ggccccgtcg cgctcgcgtt gtgcggcgcg
      781 tccgatcccg acacgcacaa gcgcatcgac ggaatcctcg ccgaacaagg cggcgccgat
      841 tttgccgcgc atttcctgcg cggcgccggg ctgcactggg cggccgaact gcttgccggt
      901 tttcccggtc ccctggcccc cgaagaagga gaaatagcat gaagacgatg atcttgggcc
      961 tcgcggccat gtcgttcgcg ccgctggcga tcggatttgc gccgcccgcg cacgcggtgc
     1021 cggtattcga cagcgccaat tattcgcaga atgtgctgac cgcggcgcgc accctccagc
     1081 agatcaacca gcagatccgg tcgctccaga acgaagccca gatgctggtc aaccagcaga
     1141 agaatctgtc gagggtcgac tttccgcaga tgtccgccct tcagcaacgc ctcgccgaga
     1201 tcgaccggtt gatggggcag gcgcaaggca tcgagtttcg cctcgaccgg ctcgacgggc
     1261 aattccgcgc cgctttcccg acaagcttcg agcatctcgc gcggcgcgac cagcgccttg
     1321 caaccgcgag ggcgcggatc gaggcggaga tggagtcctt ccgccacgcg atgagcgttc
     1381 agagcgggat cgtcgagaat gttcgcagcg acaccgagac gctgagggcg atcgtcgagc
     1441 gaagccaggg cgccgaaggc gggctgcagg ccgcgcaggc cgcgaaccag ttgctggcgc
     1501 tcgccgccaa gcagcagttc cagatccagc agctgatggc ggcacagttt cgcgaacaga
     1561 cgttcgaacg cgcgaagcgc gcgcagatcg agcgcgaagc acgcgaatcg acgcgccgct
     1621 ttctcggcga cggcaaggcg tggacgccgc cgcgctgagc ccggggcgaa agattggggc
     1681 aggacgggac acaccgagac cccgcccagg cctgccccgt cgcgggggcc gcggatgccg
     1741 tggaacggaa agaccaggcc gcttgccgcc ggcccccgcg acgaagctca tgaggaaact
     1801 gtgacggatg gacgatctca acatcatcga ccagttcatg gccgcgttcg tcgcctatat
     1861 cgacagcggg ttcgggctgc tcggacctga cgtcggcttc ctgacgacgg cgctcatcgg
     1921 catcgacatc acgctcgcgg gcctgttctg ggcgctgggg ggcgaagatg atgtcatcgc
     1981 gaaatttctc aagaagatcc tctatgtcgg cgccttcgcg ctgatcctca ataatttcca
     2041 gtcgctgtcg gacatcatct tccgctcctt cgccggcctc gggctcaccg ccggcggcag
     2101 cgcgatcgga gcgtcggacc tcctgcttcc ggggcggctc ggaggcacgg gattcgaggc
     2161 ggcgtcgccg ctgctcgaaa gcgccaagga actggtggga ccgatcgcct tcttcgaaaa
     2221 tttcgtcgaa atcgcggtcc tcctcttcgc gtggctaatc gtcatcgcgg cttttttcat
     2281 tctggcggtc cagatgttca tcaccatcct cgaattcaaa ctcaccacgc tggcgggctt
     2341 cgtcctcgtg cccttcgcgc tgtggaaccg cacggccttt ctcgccgagc gcgtgctcgg
     2401 atcggtgatc acatcgggca tcaaggtcat ggtcctcgcc gtcatcgtcg gcatcggctc
     2461 caactacttc gccgacttca cgagcgcgct cgcgggcgag gaggtcacga tcgagcaagc
     2521 cgtgtcgctg atgctcgcaa gcctcgctct cctcggcctt tccattttcg gccccgcgat
     2581 cgcatccggg ctcgtcgccg gcgccccgca gctcggcgcg ggttcgatcg ccgcaacctc
     2641 cgcgtttgcg gccggcggtg tcgtgatggc gggg
//
LOCUS       DATMSU010000375         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_6992, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000375 DATMSU010000000
VERSION     DATMSU010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6992"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VN034_10050"
     CDS             <1..245
                     /locus_tag="VN034_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWV60988.1"
                     /translation="VRKRQRLSGGRWDRTTRRPRLAHVLGITAIDTARRLRRAAITQE
                     LSLRRRLAVMVLTLQLAGVAVGEFLNLAGGRKAARD"
     gene            245..1210
                     /locus_tag="VN034_10055"
     CDS             245..1210
                     /locus_tag="VN034_10055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWV60989.1"
                     /translation="MTMSRSAPRFSVVMPLYNKAAHVRAAIESALAQSYPAHEILVID
                     NGSTDGGRELAAAIGDARIKLLDLATPGPGGYAGRNLGIRAARGDWIAFLDADDLWQP
                     DHLAVLAEGISADPDARAAATRFDHVFEDRAQPQRTAPQLDHARSLDLAEFLSAWLAV
                     RECPMWTGAIAIRRDTLFEAGLFPEGRAVRGGDKDLWLRVVAKGALRYDPRITARFHR
                     DSDNKVSKSTTTLDLPCLVETARAMLADAAGHEAALLRRLVNQEIAHYARYAMKYPGR
                     TAIRLSDLYLPEGTGTAALLLAAKLIPASLRQSGHALRNRLLARA"
     gene            1216..2121
                     /locus_tag="VN034_10060"
     CDS             1216..2121
                     /locus_tag="VN034_10060"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWV60990.1"
                     /translation="MRALGSLLTGGGIVAAWLSLAGGRVPALDLLASFLPLFGAMAML
                     GILLAKWRRGSAIAAALLALVPIAIGMAPEWLREIPAAPAGALQVHMLTHNVWSGNTD
                     PIGTAQAIIDAKPDVVMLQELNRNSWPMLAVLNPHFPYSTKCPRGCDLAILSRWPIVR
                     TDYFLKDANGRKFGPAMRLAWIKPPAGEVFMAATLHYPRPTSGNQAERRRALAQALAR
                     IDRDGLIVAGDMNLTPWAAAMREQDRAFAPLTRMTRAPSWPRVVPVLPIDHLYAGPDW
                     GLVSTRQLPATGSDHRPILVTLARR"
     gene            2118..3416
                     /locus_tag="VN034_10065"
     CDS             2118..3416
                     /locus_tag="VN034_10065"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HWV60991.1"
                     /translation="MKQVLSIALLGSCGFAAPALAQQSDATDGLGSVSSENSFGRDRN
                     ISVLERRRPDYTAEPIRVGSLELMPRIAVGAGYDDNLYAQPDGEIGDAYLRIRPRISL
                     VRPSPDLKLSLEGELDLLRYADRSSENATQYAANAGAAYTISKSDTLDVTLRNGRYSQ
                     ERVSPDSPTQASRPIRFTLNGGTATYTHVFNRLRVRGVIDVENRNYSDSVTPGGDPID
                     QDFRDHTSYTGTAIGEYALSPSVALFVAGSLGKRDYRERSGPVAARDSTGFDLAAGAS
                     FELGRKARGSLRLGYLHQDYKAAEFEDVSGFLVRGELAYFLTPLVTITGTVDRGIKET
                     GVNGATGYLATNMALRADYELLRNLIISAGGELEKRDFNNIDRQDDRWTWRANASYLV
                     SKRMALRVDLQRRTQSSWGFNAGREFTDNRVSVGVTFSGL"
     gene            complement(3423..4148)
                     /locus_tag="VN034_10070"
     CDS             complement(3423..4148)
                     /locus_tag="VN034_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HWV60992.1"
                     /translation="MDMASASLAPAPAFEEPVSEETGKPHLRGCPVSFAKLKALLAAD
                     LYRYAGRVGFGAFAKHYAFTPGYKYTVLMRTAGWLKLKPAKAYGLYPLAKWLLLRARY
                     KYGFAIPEYMEIGPGLFLNRFGGFYFHGDAVLGNNVNITHGVVLGYMNRGSRRGAPVI
                     GDRSFLGSGAKIIGGIAVGAEAAIGANAVVTKDVPERGVVGGIPAKLLSDQGSDGYIN
                     RLAPPDLLAACEGALHGSRTKPR"
     gene            complement(4148..>4647)
                     /locus_tag="VN034_10075"
     CDS             complement(4148..>4647)
                     /locus_tag="VN034_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWV60993.1"
                     /translation="APLAGSGAAPPPAEAFRLSPGDKVKIATYGEETLTGDFEVSPAG
                     TIAFPLIGEVKAAGLQTDQLSKAIEAKLGDGYLLEPKVSVQVASFRPVYVLGEVNKPG
                     EYPYTQGLTIRGAVAKADGFTYRANEKRVFLKRAGEAGEREYEMTADFPVLPGDTIRF
                     GERYF"
BASE COUNT          755 a         1571 c         1559 g          762 t
ORIGIN      
        1 tggtgcgcaa gcgccagcgg ctgagcggcg ggcgctggga ccgcaccacg cggcgcccgc
       61 gcctcgctca tgtgcttggc atcaccgcca tcgatacggc gcggcggctg cggcgcgcgg
      121 cgatcacgca agaactgtcg ctccgccgcc ggctcgcggt gatggtgctg acgttgcaac
      181 tcgccggggt ggcggtgggc gaattcctca atcttgcagg gggacgaaag gcagcgcgcg
      241 actgatgacg atgtcccgat ccgctccgcg cttttccgtt gtgatgccgc tttataacaa
      301 ggccgcgcat gtccgcgcgg cgatcgagag cgcgctcgcg cagagctacc ccgcgcatga
      361 gatactcgtc atcgacaatg gctcgaccga cggcggacgc gaactggcgg ccgcgatcgg
      421 cgacgcgcgg atcaagctgc tcgacctcgc cacgcccggg ccgggcggct atgccgggcg
      481 caacctcggc atccgggcgg cgcgcggcga ctggatcgcc ttcctcgatg ccgacgacct
      541 gtggcagccc gatcatcttg cggtgctcgc cgaaggcata agcgccgacc ccgatgcgcg
      601 cgcggcggcg acgcggttcg accatgtgtt cgaagatcgc gcgcaaccgc agcggaccgc
      661 gccgcagctc gatcacgcgc gcagcctcga cttggcggag ttcctgtcgg cgtggctcgc
      721 ggtgcgcgaa tgtccgatgt ggaccggcgc gatcgcgatc cgccgcgaca cgctgttcga
      781 ggcggggctc tttcccgaag ggcgcgcggt gcgcgggggc gacaaggatt tgtggctacg
      841 cgtggtcgcc aagggtgcgc tgcgctatga tccgcggatc accgcgcgct ttcaccgcga
      901 tagcgacaac aaggtcagca aatcgaccac gacgctcgac ctgccgtgtc tggtcgagac
      961 ggcgcgggcg atgctcgccg atgccgccgg gcacgaagcg gcattgctgc gccgtctggt
     1021 caaccaggag attgcgcatt acgcccgcta cgcaatgaaa tatccgggac gaacggcgat
     1081 ccgactaagc gacctctatc tccccgaagg gaccggaacg gcggccttgc tgctcgccgc
     1141 gaagctgatt cccgcatcgc tccggcagtc gggccatgcc ctgcgcaacc ggctgcttgc
     1201 ccgcgcatga agctggtgcg cgccctcgga tccctgctga ccggcggggg gatcgtcgcc
     1261 gcgtggctgt cgctggcggg cgggcgggtg ccggcgctcg acctgctggc gtctttcctg
     1321 ccgctctttg gagcgatggc gatgctgggg atactgcttg ccaagtggcg gcggggctcg
     1381 gcgatcgctg cggccttgct ggcgctggtg ccgatcgcga tcggcatggc gcccgaatgg
     1441 ctgcgcgaga ttcccgccgc acccgcgggc gcgctacagg ttcatatgct gacgcacaat
     1501 gtctggtcgg gcaataccga ccccatcggc accgcgcagg cgatcatcga cgccaagccc
     1561 gatgtggtga tgttgcagga gttgaaccgc aattcctggc cgatgctcgc cgttctgaac
     1621 ccgcatttcc cctattccac caagtgcccc cgcggttgcg acctagccat tctctcacgc
     1681 tggccgatcg tccgcaccga ttatttcctg aaggacgcga acggccgcaa attcggtccc
     1741 gcgatgcgcc tggcgtggat caagccgccc gccggggagg tgttcatggc ggcaacgctc
     1801 cactatcccc ggccgacctc gggcaatcag gcggagcgca ggcgcgccct ggcgcaggca
     1861 ctggcacgga tcgatcgcga tggcttgatc gttgcaggcg acatgaacct gaccccttgg
     1921 gccgcggcga tgcgtgaaca ggaccgtgcc tttgccccgc tcacccgtat gacgcgcgcg
     1981 cccagctggc ctcgcgtcgt tccggtgctg ccgatcgacc atctctatgc cgggcccgat
     2041 tgggggctgg tgtcgacgcg gcaattgccc gcgaccgggt cggaccatcg gccgattctc
     2101 gtcacgctgg cgcgccgatg aagcaggtcc tttccatcgc gctgctcggc agctgcggct
     2161 tcgccgcgcc cgcgctcgcg cagcaatcgg acgcgaccga cgggctcggt tcggtgtcga
     2221 gcgaaaacag cttcgggcgc gaccgcaata tcagcgtgct cgaacgccgc cggcccgact
     2281 acacggcgga gccgatccgt gtcggctcgc tcgaactgat gccgcgcatc gcggtcggtg
     2341 ccggctatga cgataatctc tatgcgcagc ccgatggcga gatcggcgac gcctatctgc
     2401 gtatccgccc gcgcatctcg ctggtgcgcc cgtcgccgga cctcaagctg tcgctcgaag
     2461 gcgaactcga cctgctgcgc tacgccgacc ggtcgagcga aaatgccacc caatatgcgg
     2521 cgaacgcggg cgcggcctat acgatcagca agtcggatac gctcgacgtc acgcttcgca
     2581 acgggcgcta cagccaggaa cgcgtctcgc ccgacagccc gacgcaggcg tcgcggccga
     2641 tccgcttcac gctgaacggg ggcaccgcga cctatacgca tgtcttcaac cggctgcgcg
     2701 tacgcggcgt gatcgatgtc gagaaccgca attacagcga cagcgtgacg ccgggcggcg
     2761 atccgatcga tcaggatttc cgcgaccaca ccagctatac cggcaccgcg atcggcgaat
     2821 atgcgctgag cccgagcgtc gcgctgttcg tcgctggatc gctcggcaag cgcgactatc
     2881 gcgaacgcag cggtccggtc gcggcgcgcg attcgaccgg cttcgacctt gccgccgggg
     2941 cgagcttcga actggggcgc aaggcgcgcg gatcgctccg cctcggctat cttcaccagg
     3001 attacaaggc cgccgagttc gaggatgtct cgggcttcct cgtgcgcggc gagctcgcct
     3061 atttcctgac cccgctggtg acgatcaccg gcaccgtcga ccgcggcatc aaggaaaccg
     3121 gcgtcaacgg cgccacgggc tatcttgcca ccaacatggc gctgcgcgcc gattacgagc
     3181 tgctgcgcaa tctgatcatc agcgcgggcg gcgaactcga aaagcgcgat ttcaacaaca
     3241 ttgaccggca ggacgaccgc tggacgtggc gcgccaacgc ctcctatctg gtgagcaagc
     3301 gaatggcgct gcgcgtcgat ctccaacggc gcacccagtc gagctggggt ttcaacgccg
     3361 gccgcgaatt caccgacaac cgcgtctcgg tgggggtgac cttctcgggc ctgtaggccg
     3421 gatcagcggg gcttcgtccg tgacccatgg agcgcgccct cgcaggcggc gagcagatcg
     3481 ggcggggcga gacggttgat ataaccgtcc gacccctgat cggagagcag cttggccggg
     3541 atgccgccga ccacgccgcg ctcgggcacg tccttcgtca ccaccgcatt ggcgccgatc
     3601 gccgcctcgg cgcccacggc gatgccgccg atgatcttgg cgcccgaccc cagaaagctg
     3661 cgatcgccga tcaccggcgc gccgcgccgg ctgccgcggt tcatatagcc gagcacgacg
     3721 ccgtgggtga tgttgacatt gttgccaagc accgcgtcgc cgtgaaaata gaagccgccg
     3781 aaacggttga ggaacaggcc cggcccgatc tccatatatt cggggatcgc aaagccatat
     3841 ttatagcgcg cgcggagcag cagccacttg gcgagcggat agaggccgta ggccttggcg
     3901 ggcttgagtt tgagccagcc cgccgtgcgc atcagcaccg tatatttata cccaggggta
     3961 aaggcataat gtttggcgaa agcgccgaag ccgacgcgac cggcgtagcg atagaggtcg
     4021 gcggcgagca gcgccttgag cttcgcgaag ctcaccggac agccgcgcag atgcggcttg
     4081 ccggtctcct cggaaaccgg ctcctcgaac gccggagcgg gggcgagcga tgcgctggcc
     4141 atatccatca gaaatagcgc tcgccgaaac ggatcgtgtc gcccggcagc acggggaaat
     4201 cggcggtcat ctcatattcg cgctcgccgg cttcgcccgc gcgcttcagg aagacgcgct
     4261 tctcgttggc gcgataggtg aagccgtcgg ccttggcgac cgcgccgcgg atggtgaggc
     4321 cctgcgtata gggatattcg cccggcttgt tgacctcgcc gagcacatag acgggccgga
     4381 agctcgcgac ctggaccgag actttcggct cgagcagata gccgtcgccg agcttcgctt
     4441 cgatcgcctt cgagagctgg tcggtctgta gcccggcggc tttgacctcg ccgatcagcg
     4501 gaaaggcgat cgtgccggcg ggcgagactt cgaaatcgcc ggtcagcgtc tcttcgccat
     4561 aggtcgcgat cttgaccttg tcgccgggcg agaggcggaa ggcctcggcc ggcggcggtg
     4621 ccgcgccgct gccggcgagc ggcgcct
//
LOCUS       DATMSU010000376         6600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_129993,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000376 DATMSU010000000
VERSION     DATMSU010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129993"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VN034_10080"
     CDS             <1..676
                     /locus_tag="VN034_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60994.1"
                     /translation="LLMLWRARGSFAPAFSAGLCFIAGAALMLAPWLYATNQMVGRPV
                     LTTNAAFNLYLGNNPAATGKFVSIADTPLAKDWDATRLRLGEVGNADRLQAEALDWIQ
                     ATPGRAAELAALKLAYFWQPNLPDANDFAASKAIASIRLFEVAQYGLILLIGLLAFRS
                     RRIASDGKWIFAAMIAGFWLIHAAAYIITRYRDPAIPLLIVMAAIPVAAWIERLPAQR
                     VLRDAA"
     gene            666..2150
                     /locus_tag="VN034_10085"
     CDS             666..2150
                     /locus_tag="VN034_10085"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWV60995.1"
                     /translation="MLHEQPDRAPSSAPGRANEAVPLLVDVDGTLTRADISLESFVRI
                     GRSSIAALIAVLTWLVAGRAVAKTMAARRDRIDAARLPYRQEVLHLIEQARAEGRPVI
                     LASASHWRHIRRIADHLALSDPVIATRGRSNLKGAAKLAAIRARIGADTPFDYVGDSR
                     ADSCLWREARHGWSVGHVPPRSAVERLGDLRPSPARAIIKAMRPHQWAKNALVIVPAL
                     TSGEFTKPAVLLTAVAAALLMSLIASSIYLLNDLLDIDSDRAHRTKWKRPLAYGDLPI
                     PAALGLSLLLAAVGLAGGWLLGGPALTFWLLAYMAITTAYSFRLKAVMVGDAIVLASL
                     YTIRIWIGAIAIGVALSFWLLLFSVFLFLSLAYLKRYVEMRDAIEPQRLLSGRGYVGG
                     DLDVVMMSGISAGMVAILVLALFAHDPETAEHYALPEMLWLLCLPLIYWLNRIWMMAR
                     RGEVEGDPVAFAIKDGRSLFVGAVTACIFLAALYGPAAVQLIAD"
     gene            complement(2173..3684)
                     /locus_tag="VN034_10090"
     CDS             complement(2173..3684)
                     /locus_tag="VN034_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60996.1"
                     /translation="MMTRKQGLPLSCAVLLVLLAAVPMLIVTVPGASDYPNHLARHHI
                     FAAIGQGTVLDRYFIVDWRWVGNLGVDLPVLALSSWLGVESATRLVSALIAPLTVAGI
                     LLLSRSAHGRVTGGAMLALPFAFAQPFLFGFLNYCLSVALALLVAAAWYANRRASLYT
                     SLAFGLGAVAVWTAHIMGWAILLLLVAGAELAMVRSWRDLVHRALRAAPLILPLVPLL
                     VWRAEGDGRLFWYAPHLLHAKAMNFVTALKGLSMPFDLAITASICLGAVLAFLWASGR
                     RLEPRLAAGGGLLLFAALLLPTTVLGSWGADLRLAPVAMMVMVMAIRPAERPERERLL
                     IMLGASLFVLRAGWTSVHWWQADRALQKRLVLLDEVPRGSRLGFLSAEADCDNWTLTP
                     DRKLGAYAITRRDAFSNTLFQIPGSDVMTIRQPPDRARWFDGSQDIPVRCPEGAPDRL
                     VLGERMRSMAQADFNALWIAGLDAKSVPRLPGYAIAYSRGIDTLLIKQAYGLP"
     gene            complement(3973..5091)
                     /locus_tag="VN034_10095"
     CDS             complement(3973..5091)
                     /locus_tag="VN034_10095"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWV60997.1"
                     /translation="MVSEAAAAYRNAVERAPELGAAWLGIANLRGAPFSAADICAMER
                     ALPLVHDPYQRIQMFFALGRALGDGGNFSRSFENFCEANSLRETLIPHDAARLAAFVD
                     AHRILPPPFFAVTEEISSKTPGAIFIVGMPRSGSTLVEQILASHPDVEGLGELLALEE
                     VAASMGAFDAPDAFVGRLQNLTQAEAAHLGAHYLARANRYRRTSRPYFTDKMPANWRF
                     VALIRRIVPDARIIDVRRNPMACGFSAYTTYFNRNTDFPNALEDLGRYYRSYRRMMEM
                     VQASAPDTVCDIDYERLVSDPDLEIAALLDRLRLPFAEACLAPDRERRAIYTPSAQQV
                     RTPIHRMKDQFRDYLPWLGALQASLGAEEGEPTPYVRQ"
     gene            5167..5478
                     /locus_tag="VN034_10100"
     CDS             5167..5478
                     /locus_tag="VN034_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60998.1"
                     /translation="MEIDGFVRAPLGGEDARLQRQRPDARRMAPRCMVEVEEGFFDAP
                     RVAQMGYESGVRAGGFGLRRDRTAQRLLLVGAGGMSRHGSRFGGNVMPCIYRSARSRR
                     K"
     gene            5926..>6600
                     /locus_tag="VN034_10105"
     CDS             5926..>6600
                     /locus_tag="VN034_10105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV60999.1"
                     /translation="MSRPNDRSARARSRRLLCGASLAAVLACAPLSAQAEDRHWDANV
                     TAVGSGGTGTWDLANLNWSPNGDGVSGPFVRPWENGTVDNAIFGGTAGTVTVAVPVTV
                     GNITFNSAGYVLNGSTLALDGPAPTITTNFGNANATINSAIAGSSGLVKAGVSGLILN
                     GTNSFTGGITLNSGSLYAASDAALGALSNNIVTAADVQVRLSIGGASTSRAVAIGDGG
                     ALILEGS"
BASE COUNT         1188 a         2134 c         2133 g         1145 t
ORIGIN      
        1 gctgctgatg ctctggcgcg cgcggggcag ctttgcgccg gccttctccg cgggtctgtg
       61 cttcatcgcc ggcgccgcgc tgatgctggc gccgtggctt tatgcgacca accagatggt
      121 cggccgcccg gtcctcacca ccaacgccgc gttcaacctg tatctcggga acaaccccgc
      181 tgcgaccggc aagttcgtga gcatcgccga cacgccgctg gcaaaggatt gggacgccac
      241 gcgcctgcgc cttggcgagg tcggcaatgc cgaccgcctg caagccgaag cgctcgactg
      301 gatccaagca actcccggca gggcggccga actggccgcg ctcaaactcg cctatttctg
      361 gcaacccaat cttcccgacg cgaacgattt cgcggcctcg aaggcgatcg cctcgatccg
      421 gctcttcgag gttgcgcaat atggactgat cctgctgatc gggctgctcg cctttcgttc
      481 gcggcggatc gcaagcgacg gcaaatggat ttttgctgcg atgatcgctg gcttctggct
      541 gatccacgcc gccgcctata tcatcacccg ctaccgcgat ccggcgattc cgctgctgat
      601 cgtcatggca gccattccgg tcgcggcctg gatcgagcgg cttccggcgc aaagggtctt
      661 acgcgatgct gcatgaacaa cccgaccgcg cccccagcag cgcccccggt cgcgccaacg
      721 aggccgtgcc gctgctcgtc gacgtcgacg ggacgctgac gcgcgcggat atctcgctcg
      781 aaagcttcgt gcggatcggg cgatcgagca tagccgcgct gatcgcggtg ctgacatggc
      841 tggtcgcggg ccgcgccgtc gccaagacga tggctgcgcg gcgcgaccgc atcgacgcgg
      901 cccgactgcc ttaccggcaa gaggtgctcc atctcatcga gcaggccagg gccgaaggcc
      961 gccccgtgat cctcgcgagc gcaagccatt ggcggcacat ccgccgcatc gccgatcatc
     1021 tggcgctttc ggacccggtc atcgcaacgc gcggccgctc caatctgaaa ggagccgcca
     1081 agctggccgc catccgtgca cgcatcggtg ccgatacgcc gttcgactat gtgggcgaca
     1141 gccgcgccga cagctgcctg tggcgcgaag cgcggcatgg ctggtccgtc ggccacgtcc
     1201 cgccgcgatc ggcggtcgaa cgtctcggcg acctgcgccc cagccccgcc cgggccatta
     1261 tcaaggccat gcggccccac caatgggcca aaaacgcgct ggtgatcgtt cccgcgctga
     1321 cctcgggcga attcactaaa cccgccgtat tgctcacggc ggtggccgcc gctttgctca
     1381 tgtcgctgat cgcctcgtcg atctatttgc tgaacgatct gctcgatatc gactccgatc
     1441 gcgcgcatcg taccaaatgg aaacggccgc ttgcctatgg cgatctcccg atacccgccg
     1501 cgctcggcct gtccctgctt ctcgcggccg tgggtcttgc cgggggatgg ctgctgggcg
     1561 gcccggcgct gaccttctgg ctcctggcct atatggcgat cacgaccgcc tattcgttcc
     1621 ggctgaaggc ggtgatggtc ggcgacgcca tcgtcctcgc ctcgctctac acgatccgta
     1681 tctggatcgg cgcgatcgcg atcggggtgg cactcagctt ctggctgttg ctcttctcgg
     1741 ttttcctgtt cctgagcctc gcctatctga aacgctatgt cgagatgcgc gacgcgatcg
     1801 aaccgcagcg cttgctgagc gggcgcggct atgtcggcgg cgacctcgac gtggtgatga
     1861 tgtccgggat ctcggccgga atggttgcga tcctcgtgct cgccttgttc gcgcacgacc
     1921 cggaaacggc cgaacattat gcgctgcccg aaatgctgtg gctgctctgc ctgccgctga
     1981 tctactggct gaaccgcatc tggatgatgg cgcggcgcgg cgaggtcgag ggcgacccgg
     2041 tggccttcgc gataaaggac ggacggagcc tcttcgtcgg cgcggtgacg gcctgcattt
     2101 ttttggctgc cctctacgga ccggcggccg tccagctgat cgccgattga gggcgcggaa
     2161 tcgggccggg gctcagggca gtccgtatgc ctgcttgatc aacagcgtat cgatcccgcg
     2221 gctgtaggct atcgcatagc ccggcaagcg gggaacgctc ttggcatcga gccccgcgat
     2281 ccacaaggcg ttgaaatccg cctgtgccat gcttctcatc cgctcgccga ggacaagcct
     2341 gtccggcgcg ccttcggggc agcggaccgg aatgtcctgc gagccgtcga accagcgggc
     2401 gcgatcgggc ggttgccgga tcgtcatcac gtccgacccg gggatctgga acagcgtatt
     2461 gctgaaagcg tcgcgccgcg tgatcgcata ggcgccgagc ttgcggtccg gggtcaatgt
     2521 ccagttgtcg caatccgctt ccgccgacag gaagccaagc cggctccctc gtggcacctc
     2581 gtcgagcagg accagtctct tttgcagcgc gcgatcggcc tgccaccagt gcaccgacgt
     2641 ccacccggcg cgcagcacga acaggctcgc gcccagcatg atcagcagcc gttcgcgttc
     2701 gggccgttca gcggggcgga tcgccatgac catcaccatc atcgccaccg gcgctaggcg
     2761 caggtccgca ccccagcttc ccaaaaccgt cgtcggcaac aaaagcgccg cgaacagcag
     2821 caatccgccg cccgcggcga gacgaggctc cagccggcgc ccgctcgccc acaggaatgc
     2881 aaggaccgcg cccaggcaga tgctcgccgt tatcgcgagg tcgaacggca tcgacaggcc
     2941 tttgagcgcc gtgacgaaat tcatggcctt cgcgtgcagg aggtgaggag cataccagaa
     3001 taagcggccg tcgccttcgg cgcgccagac caaaagcgga acgagcggca aaatcagcgg
     3061 cgcagcgcgc aaggcgcgat gcaccagatc gcgccagctt cgcaccatcg ccaactctgc
     3121 gccggcgaca aggaggagca agatcgccca ccccatgata tgggcggtcc ataccgcgac
     3181 ggcgccgaga ccgaaggcaa gcgacgtata aaggctcgcc cgccggttgg cataccacgc
     3241 cgccgcgacg agcagcgcca gcgccaccga caggcaatag ttgaggaaac cgaacaaaaa
     3301 gggctgcgcg aaagcgaatg gcagcgcgag catcgcgccc cccgtcacac ggccgtgcgc
     3361 ggaacgggac agcaagagga tgccggcgac ggttagcgga gcgatcagcg cgctcacgag
     3421 gcgcgtagcg gactccaccc cgagccagga cgaaagcgcc aggaccggaa ggtcgacgcc
     3481 aaggttgccg acccagcgcc agtcgacgat aaaatatcgg tcgagtaccg ttccctgccc
     3541 gatcgccgcg aagatatggt ggcgcgcgag atgattgggg taatccgatg ctccgggcac
     3601 ggtgacgatc aacatcggca cggcggcgag aaggacgagc agcaccgcgc acgagagcgg
     3661 gagcccttgt tttcgggtca tcatcccgcc gcattccttc cggcaaagac ccgtatttcg
     3721 tgtgcggaaa ctattgcgct gcccgaaagg gattcgagct gacggctcga ccgcataccg
     3781 atcaaggccg accccgcgca ggaaaaaact cgcgccgtcc catatgcatc tcgattatat
     3841 cccccgcata tacacttggc ccggacgttc caggccggcc atgctggcat gataaacgtc
     3901 catctgaaga atagatattt ttcccgaaca tgagtctgat ggtgccgagt ttatcgagcc
     3961 catgtcatct tctcattgac ggacatatgg cgtgggctct ccttcctcgg cgccgagcga
     4021 ggcttggagc gcgcctagcc aaggtagata gtcgcgaaat tggtctttca tgcgatgaat
     4081 gggcgtacgg acttgctgtg cgctcggggt ataaatggcg cgccgttctc tgtctggcgc
     4141 gaggcaggct tccgcgaaag gaagccgcaa ccgatcgagc aacgcggcga tttccagatc
     4201 cgggtccgat acaagccgtt catagtcgat atcgcaaacg gtatcgggcg ccgatgcctg
     4261 aaccatttcc atcatgcggc gataggaccg gtaatatcgg ccgagatctt ccagcgcgtt
     4321 cggaaaatcg gtgttgcggt tgaaataggt cgtgtaggct gaaaaaccgc acgccatcgg
     4381 gttccggcgc acgtcgatga tgcgggcatc cggaacgatc cggcgaatga gcgcaacaaa
     4441 gcgccagttg gcgggcatct tgtccgtgaa atagggccgg ctggttcggc gatagcggtt
     4501 ggccctcgcc agataatgtg cgcctagatg cgccgcttcg gcttgcgtca aattctggag
     4561 gcggccgaca aaggcgtcgg gagcgtcgaa agcgcccatc gatgcagcga cttcctcgag
     4621 cgcgagcaat tcgccaaggc cctcgacgtc cgggtggctg gccagaattt gttcgaccag
     4681 agtggatccc gagcgcggca tgccgacgat gaagatcgcg ccaggcgttt tggaggagat
     4741 ttcttcggtg accgcgaaaa agggcggcgg caaaattcgg tgagcgtcga caaaggcagc
     4801 gagccgggcc gcgtcgtgcg ggatcagggt ttcgcgcaag ctgttggctt cacagaagtt
     4861 ttcaaacgac cggctgaaat tccccccgtc gccgagtgcc cggccgagcg cgaagaacat
     4921 ctggatgcgt tgataggggt catgcaccag cggcaaggcc cgctccatgg cgcagatgtc
     4981 tgcggcggaa aagggtgcgc cgcgcaaatt ggcgatgcca agccatgctg cgcccagttc
     5041 gggcgcacgc tcgacggcgt tgcgataggc ggctgcggcc tccgaaacca tgccgagcgc
     5101 cgccagatca ttggcgaaat tgcaccagag tatcgggacg tcgggcgcga tggcgacgag
     5161 cttgcggtgg aaatcgacgg cttcgtccgc gcgccgctcg gcggagagga cgcccgcctt
     5221 caacgacagc ggccagatgc tcgacggatg gcgccgcgct gcatggtcga ggtggaggag
     5281 ggcttcttcg acgcgccccg cgttgcacag atgggatatg agagcggcgt gcgcgcgggc
     5341 ggattcgggt tgcgccgcga tcgcacggcg cagcgcttgc tcctcgtcgg ggcgggcggg
     5401 atgagccgtc atggatcgcg cttcgggggt aacgtcatgc cgtgcattta tcggagcgcc
     5461 cgctcgcggc gcaagtgacc cgcggcggat cggcgagacg agccgcttga tcggcgacgt
     5521 tacccacctc aatcgctcac atcgataccg taacacaccc gatttcactg tccttcggta
     5581 acctcttgat aataatttaa tttatatcgg ggtttgcgcg cttcggattt tcggggatcg
     5641 gccgcgctgg cagccgatca gatcggacaa ggtgaggcga gcgggatgtg tccggcacat
     5701 ccgaaggtga agcgcgttcg ccgtcgggtg cgggtcaccg cggccgagcc gagaaagggg
     5761 ggtgaatgat cacagatcgc acgactttgc ccgccgccct cgatcatatg atcgtcgcgc
     5821 atcgcgccgc cgcccctcgg atcaggataa ccgccgccat tctcttcagc tcgcctgcct
     5881 ggcgggcctt ttcgcgttcg aggaaaactc atggccgata accgcatgtc ccgcccaaac
     5941 gaccgcagcg cgcgggcgcg ttcgcggcga cttctttgcg gggcatcgct tgccgcggtg
     6001 ctggcatgcg cgcctctgag cgcccaggcc gaagatcgtc actgggacgc caatgtcacg
     6061 gccgtcggtt cgggcggcac aggcacatgg gacctcgcca atctcaactg gagccccaac
     6121 ggcgacgggg tcagcgggcc ctttgtccgc ccgtgggaaa atggcacggt cgacaatgcg
     6181 atctttggcg gcacggcggg cacggtgacc gtcgcggttc cggtcaccgt cggcaatatc
     6241 accttcaatt cggcgggtta tgtcctgaac ggcagcacgt tggcgctgga cggaccggcg
     6301 ccgacgatca cgaccaattt cggcaatgcc aatgctacga tcaattcggc gatcgccggc
     6361 tcatcgggcc tggtcaaggc gggcgtcagc gggctgatcc tgaacggtac caacagcttt
     6421 acgggcggca tcacgctcaa ttcgggtagc ctctacgcgg caagcgacgc cgcgctcggc
     6481 gcgctgagca acaatatcgt caccgccgcg gacgtacagg tgcggctgtc gatcggcggc
     6541 gcgtcgacga gccgcgcggt cgcgatcggc gatggcggcg cgctgatcct cgagggcagc
//
LOCUS       DATMSU010000377         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_25248, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000377 DATMSU010000000
VERSION     DATMSU010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25248"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VN034_10110"
     CDS             <1..612
                     /locus_tag="VN034_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HWV61000.1"
                     /translation="PLPRRMWAGSRIDFLAPVPVGAALTRVTTIDAIKPKRGASGALL
                     FVTLRHEISADGADAIREEQDIVFREAAGATPTLVVAPAEIPEPADAVRSITPDPVLL
                     FRYSALTFNAHRIHYDRDYARDVEGYAGLVVHGPLIATLLMGHALRAGLAPRRFAFRA
                     EAPLIDGAPFHLCLAHDAQGARLWARDAAGRTKMRAELCCTTD"
     gene            1032..1442
                     /locus_tag="VN034_10115"
     CDS             1032..1442
                     /locus_tag="VN034_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61001.1"
                     /translation="MSDKDAIAAAVTEMVDAINRGDFVTATAAFSDAPIIIEDIAPFC
                     WHGPDAVSAWLSAMGANAARLSAHAVRMRLGEPARIGMDASTAYALFPGRLSLVTADV
                     DLHADGTLTFTLQLSGERWLISALVWSGLEPAAV"
     gene            1658..2149
                     /locus_tag="VN034_10120"
     CDS             1658..2149
                     /locus_tag="VN034_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61002.1"
                     /translation="MKQSHAAELFGVDQSTVSRWECGILDMTRHQRARAETLLGARLS
                     SAGDAALARLVEQSSGGAHLMCDYSHRLLALSSMRRREFGIDADELMGRSLWPFITEE
                     LAKIETSLEAQGWFDLASPPSLVAFTGENCSDIVPIIRSHCRLTRLILSDGAPVRLIE
                     TLN"
     gene            2220..3425
                     /locus_tag="VN034_10125"
     CDS             2220..3425
                     /locus_tag="VN034_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61003.1"
                     /translation="MRRNYSIFMLVAAGTVLGLMGTDLVLPAIPVLPERLGGTASEAQ
                     LVLAAYVAGTCLGLIGCGAISERWPAKRLLVASLASMAVTSFLCTLATSLPMLTALRA
                     AQGFVAAAPAVLAPAIVKSLFDERGATRALGLLGSVESLAPALAPIAGALLLGFGGWQ
                     LSFDLLAILAAVLAVAVAEVGAFPNVATRRDGGYIRLLRDTTFLRYAVSQALVLGGLL
                     TFVFGAPAVFVRVMDGSITDFIILQMSAIATFIVAANLSGRLAVRYGAERIILFGTAL
                     AASASTAQFLYALSGGASILMISMLAIPMSTGLGLRGPPGFFRAIVAARGDDARGGAL
                     VILGILGSASAGTALVSPWIELGMTPLASVTLAFHAGALAALFFCPKLPELSGEHILA
                     GYGQDGAIG"
BASE COUNT          591 a         1140 c         1134 g          776 t
ORIGIN      
        1 ccgctcccgc gccgcatgtg ggcgggcagc cgcatcgact ttctggcgcc cgttccggtg
       61 ggcgccgcat tgacgcgcgt cacgacgatc gacgcgatca agcccaagcg cggtgcgagc
      121 ggcgccctgt tgttcgtgac gctgcgccat gagatttcgg cggatggcgc cgatgcgatc
      181 cgcgaagaac aggacatcgt gtttcgtgag gcggcgggtg cgacgccgac cctcgtcgtc
      241 gcgcccgccg aaatccccga gccggccgat gccgtccgca gcatcacgcc cgacccggtg
      301 ctgctgttcc gctactcggc gctgaccttc aacgcgcacc gcatccatta cgaccgcgac
      361 tatgcgcgcg atgtcgaagg ttacgccggc ctcgtcgtcc acggcccgct gatcgcgacg
      421 ctgctgatgg gtcatgccct gcgtgccgga ctggcccccc gccgctttgc cttccgcgcc
      481 gaagcgccgc ttatcgacgg tgcgcccttc catctctgcc tggcgcatga cgcccaaggt
      541 gcgcggctat gggcccgcga tgccgccggg cgcacgaaga tgcgcgctga gctttgttgc
      601 acaaccgatt gaggaattgc gctttcgctc gttggttgag caggtagatc gatcggcgcc
      661 caattatgcg ggcgagactt tcgcaacttg cctaatcgcc gcatgacttt tccttcgggg
      721 gcgaggatcg gtttccggtc cgggctttcc ggatcttagt ttcctcttct gaaccgattc
      781 tgctgatcaa agatgtcgga caacgctcga aaagacgcgg caaaccactc tcaagctcca
      841 cgacatcaag cgtcgtcgta ccgttccaac gtcagctttt ccaggtctgc tcctgaaagc
      901 ggaccgactg aaatcggcca gatccagcgg taaactgccg atctagcggc aatctcggcg
      961 aaacgtctcc tatggtgggg tggaaagcgg tcatcagagg gtgcaatagt ctcttacaag
     1021 ggggtgcaga catgtcggac aaagatgcca tcgccgccgc ggtcaccgaa atggtcgacg
     1081 caatcaatcg gggcgatttc gtcacggcga ccgctgcttt cagcgatgca ccgattatca
     1141 tcgaagacat cgccccattc tgctggcatg gccccgatgc cgtgtctgca tggctttcag
     1201 ccatgggagc gaacgcggcg cggctgagcg cgcacgcggt tcggatgagg ttgggggagc
     1261 ctgcccgcat cggcatggac gcgtcgaccg cctatgcctt gtttcccggc cggctcagct
     1321 tggtgacagc ggacgttgat ctacatgccg acgggacact cacatttacc ttgcagctta
     1381 gcggcgagcg gtggctgatc agcgcgctgg tatggagtgg cttggagccc gcggctgtct
     1441 gaatgtctgc aagcggtgcc tcatccgcgc gaagttctgg tgaatgtcgg ctatcttgga
     1501 ctgccgcctg aaagcggccc gaaccggaga tggaagcagc gctgttgcgg ctggtcaaac
     1561 gctctgtcat tatgccggga cggctgcgag acgatcgatg attatacccc agcgacccct
     1621 aggccaggca ctgcgtcggt ggcgcgcgct caaccgagtg aagcaatcgc acgccgccga
     1681 actattcggc gtcgatcaat caaccgtgtc gcgctgggaa tgcgggatac tggatatgac
     1741 gcggcatcag cgggcgcgcg ctgagaccct cttgggcgcc aggctttcaa gcgccggcga
     1801 tgctgcgctt gcgcgattag tggagcaaag tagcggcggc gcgcacctca tgtgtgatta
     1861 cagccaccga ctgctcgcgc tatcctcaat gcgtcggcgc gaattcggca tcgacgccga
     1921 cgagctcatg gggcgttcgt tatggccctt catcaccgaa gaacttgcca aaatcgaaac
     1981 ctccctcgaa gcgcaaggct ggttcgatct tgcgtcgccg ccttcgctgg tcgccttcac
     2041 gggggaaaat tgctccgata tcgtcccgat catacgcagc cattgcaggc taacgcggct
     2101 catcctgtcg gacggtgccc cggttcgcct aatcgagacg ctgaactagg gcgcatatct
     2161 tatgcgttgc tccggaagct gagccgcgcc acgcgcaacc tcgatctcgt gaggtgggta
     2221 tgcgcaggaa ttattcgatc ttcatgcttg tcgctgcggg gacggtgctc ggtctcatgg
     2281 gaaccgatct cgtgctgccc gccattccgg tccttcccga acgcctcggt ggcaccgcct
     2341 cggaggctca gttggtgctc gcggcctatg tcgcggggac ttgtctcggg ctgatcggct
     2401 gtggcgcgat cagcgagcga tggccggcaa agcggctcct ggtcgcatcg ctcgccagta
     2461 tggccgttac gtccttcctt tgcactctcg ccacctcgct cccgatgctt acagcattgc
     2521 gcgcggcaca gggctttgtc gcagcggcgc ccgctgtact cgcgccagcg atcgtgaagt
     2581 cgctgttcga tgagcgaggc gctacgcgtg cgctcggcct gctggggagc gttgaatctc
     2641 tcgctccggc actcgcgccg attgcgggcg ctctgctcct cgggttcggc ggatggcagc
     2701 tgtcattcga cttgcttgcg atcctggccg cagtgcttgc ggtcgctgtc gcagaggtgg
     2761 gcgcctttcc caacgtcgca acgcggcgcg acgggggata tattcggctt ttgcgcgaca
     2821 cgactttcct tcgctacgct gtcagccagg cgctggtcct gggtgggctg ctcacatttg
     2881 tctttggtgc tcccgctgtt ttcgtacggg tgatggatgg atccatcacc gatttcatca
     2941 tcctgcagat gagcgcgatc gcgaccttca tcgtcgcggc caacctgtcc ggtcggctag
     3001 ctgttcgata tggagccgag cggatcatcc ttttcggaac ggcgctcgca gcctccgcat
     3061 cgaccgcgca atttctctac gcgctttcgg gcggggcgag catcttgatg atctccatgc
     3121 tggccattcc gatgagcacc gggctgggat tgcgcggccc gccgggcttt ttcagagcaa
     3181 ttgtcgccgc ccgtggcgac gacgcccggg gaggcgcgct cgtgatattg ggtatcctgg
     3241 gctccgcctc ggctggaacc gcgctggtgt cgccttggat cgagttaggt atgacgccac
     3301 tcgcctccgt cactctggct ttccatgcag gcgcactcgc cgctttgttc ttttgcccaa
     3361 agcttccgga attgtcgggg gaacacattc tcgcagggta tggtcaagat ggcgcgatcg
     3421 gttgacgaac gctgtgggcg agaagcgaat gtccgcttcg tccggttagg atgataagag
     3481 ccgaccgacc gcatccggcc agttgcgact acgcgatctt tggagctttt gctggtaccg
     3541 ctaagtcgtt ctcacctgca accggcgaag ccggtgaacg cgtccttcgc aatcttgtta
     3601 tccccggcgt tcaggctctg acaatctgcg cgatgccaat g
//
LOCUS       DATMSU010000378         8899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_139386,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000378 DATMSU010000000
VERSION     DATMSU010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139386"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VN034_10130"
     CDS             <1..251
                     /locus_tag="VN034_10130"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HWV61004.1"
                     /translation="GYSGMLAGTLGLNHGEFPKSPVLLVHGTADPVVPIAALHMSESE
                     LRRLGVEVTTHVSYGVAHTVDPVGLRLGRDFVAEAFAR"
     gene            615..1226
                     /locus_tag="VN034_10135"
     CDS             615..1226
                     /locus_tag="VN034_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61005.1"
                     /translation="MDLNQLLHHHQMALMAVSQARREGQAMPNFDLPRYYAKRINEYR
                     ERRGLSGDAEAIADLAVSPAGAVEAPDDLVSDVLIAELLMAKAFEKATHQHRADGSVG
                     ADDPVVDIRATLLAVGRDFYRSLGVEDGPLAAAIDEAASRSAWEGEGGGLRPPGDEED
                     GIIRTYVEQFAVGDYRYSNLADAKAAARRQRDAASDGRGATPA"
     gene            1223..1408
                     /locus_tag="VN034_10140"
                     /pseudo
     CDS             1223..1408
                     /locus_tag="VN034_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(1526..1660)
                     /locus_tag="VN034_10145"
     CDS             complement(1526..1660)
                     /locus_tag="VN034_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61006.1"
                     /translation="MAKSQKRSNREIRKPKAAKAKPPTALAAQEASAVQMTAKPKGNR
                     "
     gene            complement(2042..2275)
                     /locus_tag="VN034_10150"
     CDS             complement(2042..2275)
                     /locus_tag="VN034_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61007.1"
                     /translation="MSRAINISLPEDEVKRLCLLQGVSISAIEPLQSGGTRLVCTSGS
                     GAEEMRLRLRGHLIDGNVLRYRFYRPPATHERP"
     gene            2953..3564
                     /locus_tag="VN034_10155"
     CDS             2953..3564
                     /locus_tag="VN034_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWV61008.1"
                     /translation="MHAPPILYSFRRCPYAMRGRMALIASGVAYEHREILLRDKPAAM
                     LAASPKGTVPVLVLANGQVIDESIDIMRWALDQNDPEGWMTAAEPCLVADYDGAFKYH
                     LDRYKYAVRYGEDPLMHRAAGLTMLCRLDAQLAGRDYLSGSARGFGDIAIFPFVRQFA
                     GVDPAWFEAQAPQKLQGWLERLISSDLFERAMIRRTLWTADET"
     gene            3611..4282
                     /locus_tag="VN034_10160"
     CDS             3611..4282
                     /locus_tag="VN034_10160"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV61009.1"
                     /translation="MERMSLSKIVNGASLKPRSGEPPKKIVLLLHGFGASGTDMIALA
                     PTWREALPDALFLAPHAPQRCGMMGRGYQWWGLSGFAPSALAAGTASAAPAIDAFIGR
                     KLAQYGLSEADVAIVGFSQGTMVGLHVALRRPRAVAAVVGYSGMLAGTLGLNHGEFPK
                     PPVLLVHGTADPVVPIAALHMSESELKRLGVEVTTHVSHGVAHTVDSVGLRLGRDFVA
                     EAFAG"
     gene            complement(4589..5659)
                     /locus_tag="VN034_10165"
     CDS             complement(4589..5659)
                     /locus_tag="VN034_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HWV61010.1"
                     /translation="MAADNPPINLGRTLGLALVLASDAPLLLLDENLAIVAASDSFCA
                     AFGIDPETVTGQTIYDLDHHHWDLPRLHSLIDATLSGAAEVEAYEMEIETSAGAVRCV
                     VIKAQRLDYGDGLNTRMMVTVIDVTEERRNEKRQKELIHANAELLHEMQHRVANSLQI
                     IASILMQSARRVQSDEARGHLSDAHQRVMSIATLQRHLAESDGGAVELEPYLAQLCAS
                     IGASMIYDREQLTLTTDVDPVSISGDISVSMGLVVTELVINALKHAFPDGRPGQIVVG
                     YAAQGDDWTLSVSDDGVGMDEDPERSTAGLGTRIVQALAKQLGAEIVVEDTAPGTRVS
                     LTRTARIARQVAANDEAQIAAV"
     gene            6331..7083
                     /locus_tag="VN034_10170"
     CDS             6331..7083
                     /locus_tag="VN034_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61011.1"
                     /translation="MIEPASLRWPASASGLDDAARVEHSRGMNRKSSQRPKGLRQFLN
                     PGEQNDWLEGRTEPADAGERQDSIEQRIRYAPRFQKLLRRPQASELFQILQLYGRTCL
                     PIPRATERYYWSVSCLPSTSDKPLVRVNASWMELFTLYADGDDLRARFIVHLSDCTTD
                     GSLAPERVDRAFLEQSVRAPEDLSYSFPNGSDIFVIKVRGSASIRDFLAAPRTLRAVR
                     RFNLTHMNRGRNAYQASHSYSVADYMLGEGDA"
     gene            complement(7128..8372)
                     /locus_tag="VN034_10175"
     CDS             complement(7128..8372)
                     /locus_tag="VN034_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61012.1"
                     /translation="MTEIELQAELNDLRMSKEAQPLFDAVKRHIAENVDPITEEFFRL
                     GEGRADRWSWAPGQLELLEGAKNKAKAAGLWNFFLPHGDTGTPLTNLDYAYIAAELGK
                     SPLASESLNCSAPDTGNMEVLEMVGTPEQKDRWLKPLLNGEIRSAYVMTEPGVASSDA
                     SNLETSAHLEDDEWVINGEKYFISGAGDPRCKILICMLKTNPEASRKGQHSQILVPMD
                     TPGVQVLGPMRVFGADHAPQGHMHMRFDNVRVPRENILLGEGRGFEISQMRLGPGRIH
                     HCMRTIGKAEVALDLMVKRGNSRTAFGRPLSQLGKNLEVIARARVEIEAMRLIVLKAA
                     KAMDLLGNREARVWVSMAKAMVPERVCQIIDQAIQIHGATGISHWTPLADLYADVRHL
                     RFADGPDEVHYMVVGRDELARH"
     gene            complement(8476..>8899)
                     /locus_tag="VN034_10180"
     CDS             complement(8476..>8899)
                     /locus_tag="VN034_10180"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWV61013.1"
                     /translation="RKGHPRVGGAIDLDTYLALDHIVATARPYGPGIEDMALDRMGLA
                     RRVAVRCQHAITAWQIVAASDMLFALPKSHAAVLDAMWPMQLLDLPLPVEPGGSYLYW
                     HQAAQADPGLGWLRAIIVEQLGDSSRVVIPAKAGISPG"
BASE COUNT         1553 a         2696 c         2878 g         1772 t
ORIGIN      
        1 ttggatattc cggtatgtta gcagggacgc tggggctgaa ccacggagag ttcccgaagt
       61 cgcccgtgct attggtgcac ggcacggccg atccggttgt ccctatcgcc gcgctacaca
      121 tgtcggagag tgaactcaga cgcctcggcg tcgaggtcac gacgcacgtc tcctacggcg
      181 tggcgcacac cgttgatccc gttggacttc gcctcggccg cgatttcgtg gcggaggcct
      241 tcgctcgtta gtccgctttt ccccgtccgg cccctaaacc ggcctgccgc atttcggcca
      301 aacatgtttg tgatgacgcc ccagcaacat gggcaaaagg gcttcgctac cgcgaagccc
      361 ttccatcctc tgcgttcgtg accaaaggaa gccccatcaa ctttccaggt cgcatggaaa
      421 gtctctgcct gtcggtgcta ggccttttcc gaagaaagtc gagacatatg acccggcttt
      481 cgtcggtcgc ctacaattat aaatgtccgc tcattgcaat cgggagcctg tgaccccact
      541 tctataatat cgcccgcttc caccgcgtgc tggcgactga gagaccattg cgctcccgct
      601 gaaggagcaa aatcatggac ctcaaccaac ttctccacca ccatcagatg gccttgatgg
      661 ccgtgagcca agcgcgacgc gaggggcagg cgatgccaaa ctttgacctg ccgcgctatt
      721 atgccaaaag gatcaacgaa tatcgcgagc ggcgcggctt gagcggcgat gccgaggcta
      781 tcgccgatct cgctgtatca cctgccggcg ccgttgaagc gccggacgat ctggtcagcg
      841 acgtactgat cgccgaattg ctcatggcga aggccttcga aaaggcgaca caccaacatc
      901 gcgctgacgg aagcgtcgga gccgacgatc cggtcgtcga tattcgcgcc acccttctgg
      961 ccgtcgggcg cgacttttat cgctctctgg gcgtggagga cggccctctc gccgccgcga
     1021 tcgatgaagc tgcgtcacgc tcggcgtggg aaggcgaggg cggaggcctg cgtccaccgg
     1081 gcgacgagga ggatggcatc atccggacct atgtcgaaca atttgcggtg ggcgactatc
     1141 gctattcgaa ccttgccgat gccaaagccg ccgcgcggcg gcagcgtgac gcggcgagcg
     1201 acgggcgcgg agcgacgcct gcatgaaacg gctgattgcg cttgatcttg atggaacgct
     1261 tgccgaaagc aagcagccga tagaacccgc aatgggtgag gcgcttgccg atctcctcgg
     1321 cgtggcagat gtcgcggtga tatccggtgg cgactggccg caattcgcaa aacaggtggc
     1381 gatgcggctt cccgagcgcg cggaccttgg ggcaatggca tcaccttctc gatgatggcc
     1441 gcgcagatgc tgcgcggcct gatttgcggc tatggtgatc ccgacgccga tctggtcagc
     1501 ttccaccgca gtttttgacg gcgcgtcagc gattgccctt cggcttggcg gtcatctgca
     1561 ccgcggaggc ctcttgggct gcgagcgcgg tcggcggctt cgccttggcg gctttgggtt
     1621 tgcgaatctc gcgattgctg cgtttttgcg atttggccat gtcagatatc tttcttcggt
     1681 ttcaggatgc gcgtgttcgc gaacgtgcta gcgagccgtt tgcgtccgca atctgttgag
     1741 cggaaaatag gggcgatgct ggtagggaaa ggccgtcaga cgccgaccga tcccttcgat
     1801 caggcgccca tatcggtcga tcgccgaggg atttgtcgcg gcggcgtatc gcacgagcgc
     1861 gatctgctgg tgtatgctcc tgcatcaagc gagagcacgg atcgtccctc tgtcacatgg
     1921 agcttgttcg atttcaggtg atggcaggcc tatagcacat cccgataaat gtgccgcacg
     1981 aataccgaca ttattcgggt tcgggagaga cgttcgctca ccacgaaatc gcgcgccggc
     2041 cttatgggcg ctcatgcgtg gccggtgggc gatagaagcg gtaacggagg acattgccgt
     2101 cgatcaggtg accgcggagc cggaggcgca tttcctcggc gccggagccg gatgtgcaga
     2161 caaggcgtgt accgcccgac tgaagcggct cgatcgcact gatgctgaca ccttgcagaa
     2221 ggcacaggcg cttgacctcg tcttccggca gcgaaatgtt tatcgcacgg ctcatttgaa
     2281 tcctgggccg ctcggccagc cgcggatcgc tgccgcaacc agttcatatc cctcggagct
     2341 cccggtcgcg attgtgagcg ggcggccgcc gagcgcggcg ttgtcggtgt tcaggaaggc
     2401 tatcgccgga tcgcgtccgc ccatcgccag gaaggcgagc tgactgatgt ccccctgacg
     2461 ccgtgcgtca tcgagggcga atggactgcg gctcttacgg aaaaatcggc tcgattgcgg
     2521 tctcaatggg acaggcgcgt ccggggcggc cgactgggat tgatctggat cggggggcat
     2581 gggcgcggct cctgcttgta agcgggagca cgaggtctct cagccgcggc tgcctacgat
     2641 gaacgtgtgc cacgatgatc gctatatggg agctcgggcc aacattataa gatgtcgcgc
     2701 cgatcatctg acgaatatcg ggaatctgag cccgtcagtg tctattcggt ttctgcgaaa
     2761 tgtgattgcg cttcgattgc acgaaccagt acggagattg gccttcctaa cgaacgccag
     2821 ggaatatttg gagcgtccgc taatttcgtt ccaccgccca aagccgccaa tacgcaatag
     2881 gccagtttga gtcggcgcgt tatccggatg gggctgtacg ttcgcactgc cgtttcgcta
     2941 tgccgctggc aaatgcacgc accgccgatc ctctacagct ttcgccgctg tccttatgcg
     3001 atgcgtgggc gtatggcgct gatcgcgagc ggcgttgcct atgaacatcg agaaatattg
     3061 ctgcgcgaca agccggctgc gatgctggcc gcctccccca agggcaccgt gccggtgctc
     3121 gtcttggcga acggccaggt gatcgacgag agcatcgata tcatgcgctg ggcattggat
     3181 cagaacgatc ccgaagggtg gatgacggct gccgagccgt gcctggtcgc ggactatgac
     3241 ggcgctttca aatatcatct cgaccgctac aaatatgcag tgcgctatgg tgaggatccg
     3301 ctgatgcatc gcgccgccgg tctgaccatg ctctgtcggc tcgacgcgca gctcgccggc
     3361 cgggattatc tcagcggcag tgcgcgaggg tttggtgaca tcgcgatctt tcccttcgtg
     3421 cgccagtttg cgggcgttga tccggcatgg ttcgaagcgc aggcgccgca gaagctgcag
     3481 ggctggctcg agagattgat ctcgtcggac ctgttcgaac gcgcgatgat ccggcggacg
     3541 ctttggacgg cggacgaaac ctgacgtgga ggcgctgacg ccttgctgac aaattgccag
     3601 cgatacccat atggaacgca tgagcttgtc caagatcgtg aacggagcca gcctcaagcc
     3661 gcgctccgga gagccgccca agaaaatcgt cctgctactg cacggcttcg gggcgagcgg
     3721 aacagacatg atcgcgctcg cgccaacgtg gcgggaggcg cttccggacg cgctgtttct
     3781 ggccccgcac gcgccgcagc gctgcggcat gatggggagg ggctatcaat ggtgggggct
     3841 ttccggcttt gcgccgtccg cgctcgccgc cggcaccgcc tcggccgcgc cggcgatcga
     3901 cgccttcatt ggccgcaagc tcgcacaata tggtctgagc gaggctgacg tggccatcgt
     3961 cggcttcagt caggggacga tggtgggact gcatgtcgcg cttcggcgac cgcgggcggt
     4021 cgccgccgtc gttggctatt ccggcatgtt ggcggggacg ctggggctga atcacggaga
     4081 gtttccgaag ccgcccgtgc tcctggtgca cggcacggcc gatccggttg tcccgatcgc
     4141 cgcactacac atgtcggaga gcgagctcaa acgcctcggc gtcgaggtca cgacgcacgt
     4201 ctcccacggc gtggcgcaca ccgtggattc cgtcggactt cgcctcggcc gagatttcgt
     4261 ggcggaagcc ttcgccggct agttcggttt ccgccgtccg ggcccgagac ctgactgcct
     4321 cggttcggcc gctctcggtt ggacagagaa tgccggtcga cgccaatcag accgcgctgc
     4381 cttagcagtt gcccttaact ccatcggtac agtcgccaac ggattcgacg tcttggttac
     4441 cggcatccca agagtcggct gtccgtcatc ggccaggact agtggttgat gttctgtcgg
     4501 cagattatgc cggcgaaacc tcgccggtgg ggtgtcggcg gatgacacga gggtcctgaa
     4561 ttcccgctcc cgatcgaggg agggcagatc agaccgcggc tatctgtgct tcgtcattgg
     4621 cggcgacttg ccgtgcgatc cgcgcggtgc gggtcagtga cacgcgcgtt cccggcgcgg
     4681 tgtcctcgac tacaatctcc gcccccaatt gttttgcgag tgcctggacg atgcgcgtgc
     4741 ccagtccagc agtggatcgt tcgggatcct cgtccatgcc gactccgtcg tcggacaccg
     4801 agagcgtcca gtcgtcgccc tgtgcggcgt agccgacgac gatctggccg gggcggccat
     4861 ccggaaaagc gtgcttgagc gcgttgatca ccagttcggt gacgaccagc cccatgctga
     4921 ccgatatatc gccgctgatc gataccggat cgacgtcggt ggtcagcgtc aattgctcgc
     4981 ggtcatagat catcgacgcg ccgatgctgg cgcacagctg cgccagatag ggttcgagct
     5041 cgaccgctcc gccgtcggat tcggcgaggt ggcgctgcag cgtcgcgatc gacatcacgc
     5101 gctgatgcgc gtcgctcaaa tggccgcgcg cctcgtccga ctgcaccctg cgcgcgctct
     5161 gcatcaatat gctcgcgatg atctgcaggc tgttggcgac gcggtgctgc atctcgtgga
     5221 ggagctcggc attggcgtga atcagttcct tttggcgctt ctcattcctc cgctcctcgg
     5281 tgacgtcgat caccgtgacc atcatccgcg tgttcaggcc gtcgccataa tcgagccgct
     5341 gcgccttgat cacgacgcat cgcaccgccc ccgcgctcgt ctcgatctcc atctcatagg
     5401 cttcgacctc cgcggcgccc gacagcgtcg cgtcgatcag cgagtgcagc cgcggcaaat
     5461 cccaatgatg atggtcgagg tcatagatcg tctggccggt gacggtttcg gggtcgatac
     5521 cgaaggcggc acagaaactg tcgctcgccg cgacgatcgc cagattttca tcgagcagca
     5581 gcagcggggc atcggacgcg agcaccagcg ccagccccag cgtgcggccc agattgatag
     5641 gtggattgtc ggcagccatg cggcgcgcct ttccggtcga cccggaccgc aggaagcggc
     5701 cttgtcggca accaaataac ggggcgagtg gggtcgatgt ctccgaccct tgccccgaat
     5761 gccgcagact atagcatgcc ggctgggtga aagatgtacg gaatcgacac gtcagttcgc
     5821 tccgcaagcg ggagggaagg ctatgggcac cgtcggtcga ttaacgtggt ggccgctgcg
     5881 aggcgcccaa atgtcggctg tcggggattt atgcctgaaa ggaagctgga cggcggccgg
     5941 gaccagcgac cgccgtccta cttgatgccg catgtgtgtg ggggaacagg gggtagtgtc
     6001 tgcgctgcgt gggcgtctca ttgacacact tgcctttctc ggcaagctgt ttcccaatgc
     6061 gtcacgaccg atacggaatg gattgaagac ggcccgcttt tcggattcga gccttcgatt
     6121 gccgtgtccg aaaacgcctt gccgggatcg gccacagtct gcccaaaacc aaactcagcg
     6181 tatctttttg tgaatcgaac aaccgttaga ccgttagaaa tgtgggggta agtgtggggg
     6241 taggaattct agttcaaagt aaaaataaaa tcagatcaat gctttaatgt atgggttcta
     6301 tacaagccct gccaccatcc tccaggcagc atgatcgaac cagcgagtct tcgctggccc
     6361 gcatcggcaa gcgggcttga tgatgctgcg cgggtcgagc attctcgggg catgaacagg
     6421 aagtccagcc agaggcccaa ggggctgcgt caatttttga atcccggtga gcagaacgat
     6481 tggctggagg gacggacgga accggccgac gccggggaac ggcaggattc gatcgagcag
     6541 cggattagat acgcgccccg ctttcagaag ctgctgcgtc gcccgcaggc gtccgagctg
     6601 ttccaaatcc tgcagctata cggccggacc tgccttccga taccccgcgc gaccgagcga
     6661 tattattggt cggtttcctg cctgccatcc acgtcggaca agcccttggt gcgcgtcaat
     6721 gcgagctgga tggagctgtt caccctctat gccgacggcg acgatcttcg cgcccggttc
     6781 atcgtccatc tttcggattg cacaaccgac gggtcgcttg cgccggagcg ggtggaccgg
     6841 gcctttcttg aacagagcgt gcgggcgccc gaggatctga gctattcgtt tccgaacggt
     6901 tcggacatct tcgtcatcaa ggtgcggggc tccgcctcca tccgcgattt cctcgccgcc
     6961 cctcgcacgt tgcgcgctgt ccggcgattc aatctgacgc atatgaaccg cggcaggaac
     7021 gcctatcagg cgagccattc ttacagcgtg gcggattata tgctggggga aggcgatgcc
     7081 tgacgcgcga taattgggcg ggcggcagcc gccgcccgcc cgcgtcttta gtggcgcgcg
     7141 agttcgtcgc ggccgacgac catatagtgg acctcgtcgg ggccgtcggc gaagcgcagg
     7201 tggcggacgt cggcgtagag gtcggcgagc ggggtccagt gcgagatgcc cgtcgcgccg
     7261 tggatctgga tcgcctgatc gatgatctgg cagacgcgtt cggggaccat cgccttcgcc
     7321 atgctgaccc agacgcgcgc ctcgcggttg ccgagcaggt ccatcgcctt tgccgccttg
     7381 aggacgatca ggcgcatcgc ctcgatctcg acgcgggcgc gggcgatcac ttcgagattc
     7441 ttgccgagct gcgacagcgg gcggccgaag gcggtgcgcg aattgccgcg cttcaccatc
     7501 aggtcgagcg cgacctcggc cttgccgatc gtgcgcatgc agtgatggat gcggccgggg
     7561 ccgaggcgca tctgcgaaat ctcgaacccg cggccctcgc cgagcaggat attctcgcgg
     7621 ggcacgcgga cattgtcgaa gcgcatgtgc atatgcccct gcggcgcatg gtcggcgccg
     7681 aacacccgca tcggccccag cacctgcacg cccggcgtgt ccatcggcac cagaatctgc
     7741 gaatgctggc cctttcgtga ggcttcgggg ttggtcttga gcatgcagat caggatcttg
     7801 cagcgcggat cgccggcgcc gctgatgaaa tatttctcgc cattgatgac ccattcgtcg
     7861 tcttcgaggt gcgcgctcgt ttcgaggttg ctcgcgtcgg acgaggcgac gccgggttcg
     7921 gtcatcacat aggccgagcg gatttcgccg ttgagcaggg gtttgagcca gcggtccttc
     7981 tgctcggggg tgccgaccat ttcgagcacc tccatattgc ccgtgtcggg cgccgagcag
     8041 ttcagcgatt cggacgcgag cggcgacttg ccgagttcgg cggcgatata ggcatagtcg
     8101 aggttggtga gcggggtgcc ggtgtcgccg tgcggcagaa agaaattcca gagacccgcg
     8161 gccttcgcct tgttcttcgc gccttcgagc agttcgagct gccccggcgc ccagctccag
     8221 cgatcggcac ggccttcgcc gaggcggaag aattcctcgg tgattggatc gacattctcg
     8281 gcgatgtggc gcttcaccgc gtcgaagagc ggctgcgctt ccttcgacat tctgaggtcg
     8341 ttgagttcgg cctgaagttc gatttcggtc atttgggtct ccggtggatt ttaccggaga
     8401 agctttagtg gcgttacgct atcaatgaaa atgataggtt ggaatattcg atattcgcag
     8461 agtgaatatc tttcgtcatc ccggcgagat cccggccttc gccgggatga cgacgcggga
     8521 cgagtcgccc aactgctcga caatgatcgc gcgcagccag ccgaggccgg gatcggcctg
     8581 cgccgcctga tgccagtaaa gatagctgcc gccgggttcg accggcagcg gcaggtcgag
     8641 aagctgcatc ggccacatcg cgtcgagcac cgcggcgtgc gatttgggga gcgcgaagag
     8701 catgtcggac gcggcgacga tctgccacgc ggtgatcgcg tgctggcagc gcaccgcgac
     8761 gcggcgcgcg aggcccatgc ggtcgagcgc catatcctcg atcccggggc catagggccg
     8821 cgccgtcgcg acgatatggt cgagcgccag ataggtatcg aggtcgatcg cgccgccgac
     8881 gcgcggatgc cccttgcgc
//
LOCUS       DATMSU010000379         5924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_120643,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000379 DATMSU010000000
VERSION     DATMSU010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120643"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VN034_10185"
     CDS             complement(<1..246)
                     /locus_tag="VN034_10185"
                     /inference="COORDINATES: protein motif:HMM:NF021512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2239 family protein"
                     /protein_id="HWV61014.1"
                     /translation="MTHDPDTVTAFLGDTQLAAGRRETVTRTLEGGYPADLGAILVFD
                     DRSGRLTDLDYWDAAQGTPAPAPAPSAGRPRLGVKARE"
     gene            complement(306..932)
                     /gene="rdgB"
                     /locus_tag="VN034_10190"
     CDS             complement(306..932)
                     /gene="rdgB"
                     /locus_tag="VN034_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HWV61015.1"
                     /translation="MTRKLAPGKLVIASHNAGKVREIRALLEPYGIEPVSAGDLGLSE
                     PEETGKTFRENALLKAHASASAAGLPALADDSGLEVSALGGRPGVYTADWAERQWFEG
                     NPGRDWYMAMGKVEGLLAEQGPDVDRSACFVCTLALAWPDSHAEVFEGRAQGNLTWPP
                     RGKLGFGYDPVFVPVGNSLTYAEIAPHEKHAISHRADAFAKLVAGAFD"
     gene            complement(932..1648)
                     /gene="rph"
                     /locus_tag="VN034_10195"
     CDS             complement(932..1648)
                     /gene="rph"
                     /locus_tag="VN034_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWV61016.1"
                     /translation="MRPSGRAPDQMRPIAIETEFTIHAEGSVLVSFGNTKVLVTASVD
                     EKVPSWMRGKGAGWVTAEYGMLPRATHTRGSREAAKGKQSGRTQEIQRLIGRSLRAVV
                     DMKKLGERQIIIDCDVIQADGGTRTASISGAWVAMRLAVDKLLAAKTLTEDPIEDKVG
                     AISCGIYRGTPVLDLDYDEDSVAEADGNFVLTGKGQIVEVQASAEGATYDEEGLLRLL
                     RLARIGCGEIFAAQDRATGR"
     gene            complement(1697..2497)
                     /locus_tag="VN034_10200"
     CDS             complement(1697..2497)
                     /locus_tag="VN034_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV61017.1"
                     /translation="MTTRPLNQDDPLDDFDHRDIVLLGRKHRVYTMGSGPAVVVMTEM
                     PGISPHVARFARWVRDAGFTVYMPHIFGKDGGVVRMPGALFTMIGGCISRQFRAFQAN
                     ESSPATRWLRALAALAHKECGGKGVGAIGMCFTGNFALSMMLEPAVLAPVLAQPSLPL
                     NDPAGMHIAPDELAEVKARMEAEDLTMLAYRFEGDKYCKAARFAAYEDALGDRFIGRV
                     LPDSAANPDTPYKNPHSVVTLHLIDEVGQPTVQARDEILEFFKMRLSE"
     gene            2632..3675
                     /gene="hrcA"
                     /locus_tag="VN034_10205"
     CDS             2632..3675
                     /gene="hrcA"
                     /locus_tag="VN034_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWV61018.1"
                     /translation="MTTPPITELTTRARDVFRLVVDAYLETGQPVGSRTLSKLAALNL
                     SPASIRNVMQDLEEYGLLASPHTSAGRLPTEQGLRLFVDGMMQVAEPSAEDRAQIEAS
                     LSDAGPIESALAQATSALSGLSACAGLVLVPKHERVLKQLAFVPLSANQSLVVLVAGD
                     GTVENRVIDIPPGLNPSALVEAGNYISATLSGLTLTEAMARVRREIEAERIAIDRAAQ
                     DLVSRGLAIWSSDGADRPVLIVRGQANLLDESAVGDLDRVRQLLDELETKQDIAQLLD
                     SAREGAATRIFIGSENKLFSLSGSSVIAAPYRGSDGRVVGVVGVIGPTRLNYARIVPM
                     VDFTAQSLSRLIR"
     gene            3680..4231
                     /gene="grpE"
                     /locus_tag="VN034_10210"
     CDS             3680..4231
                     /gene="grpE"
                     /locus_tag="VN034_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWV61019.1"
                     /translation="MTNIENDTPVDDGANTENETTETAAPEGANDEVAQLAEQVAALQ
                     QDLLYARAETQNVTRRKDKEIADAHAYASTKFARDILSVADNLGRALAALSEEQRSDE
                     GIKPLITGLEATERELLSVFERNGITRIAAIGLPLDPNQHQAMLEIPSDKTPGTIVQE
                     MQAGYMMKDRLLRPAMVGVAKAG"
     gene            complement(4404..4937)
                     /gene="def"
                     /locus_tag="VN034_10215"
     CDS             complement(4404..4937)
                     /gene="def"
                     /locus_tag="VN034_10215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWV61020.1"
                     /translation="MAILPIIETPDPRLRVISKPVETFDAELKQLVADMFETMYDAPG
                     IGLAAIQVAVPKRILVIDLQEPDPDDEEGKKVIREPRVFINPVFSDESEEHSVYQEGC
                     LSVPEQYADVTRPAEVTVDWQDEDGKHHQERMTGLMATCIQHEHDHLEGILFIDHLSR
                     LKREMVLKKLAKLRKAA"
     gene            complement(5256..5852)
                     /gene="recR"
                     /locus_tag="VN034_10220"
     CDS             complement(5256..5852)
                     /gene="recR"
                     /locus_tag="VN034_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWV61021.1"
                     /translation="MASTEIEALVQQLARLPGLGPRSARRAVLHLMKKRESSFAPLLA
                     ALQTVSERLVTCGICGNVDTQDPCAICADPRRDARSLCVVEEVSDLWALDKSRLFPGK
                     YHVLGGRLSALEGIRPQDLSIDALVARVAAGGIDEVVLAMNATLEGQTTAHYLAERLE
                     GYPVRLTQLAHGLPVGGELDYLDEGTLAQALRARRPVG"
BASE COUNT         1039 a         1969 c         1876 g         1040 t
ORIGIN      
        1 ctcgcgcgcc ttgacgccca gccgcgggcg tccggccgac ggcgcgggcg ccggcgctgg
       61 cgttccttgg gcggcatccc aatagtcgag gtcggtcaaa cggccgctgc ggtcgtcgaa
      121 aacgaggatc gcgccgagat cggcgggata gccgccctca agcgtccggg tgacggtttc
      181 gcgcctgccc gcggcaagct gggtatcgcc gaggaaggcg gtgacggtgt cagggtcatg
      241 ggtcatgccg cccctttata cccgggtaaa atttctgtca atatcacccg ggtaaaaaca
      301 tcgccctaat cgaacgctcc ggccaccagc ttggcgaagg catcggcccg atggcttatc
      361 gcatgcttct cgtggggagc aatttccgca taggtcagcg agttaccgac cggaacgaac
      421 accggatcgt aaccaaagcc gagtttaccg cgtggcggcc aagtcaggtt tccttgcgcc
      481 cggccctcga aaacctcggc atggctatcg ggccacgcca gcgcgagggt gcagacgaag
      541 cacgcgctgc ggtcgacgtc cggcccctgt tcggccagca ggccttcgac cttgcccatt
      601 gccatatacc agtcgcgacc cggattgccc tcgaaccatt gacgctcggc ccagtcggcg
      661 gtgtagacgc ccgggcgtcc cccaagcgct gaaacctcga gcccgctgtc gtcggcgagc
      721 gcgggaagcc ccgcggccga cgcgctcgca tgcgccttca gcagcgcatt ctcgcgaaac
      781 gtttttcccg tctcttcggg ctcggaaagc cccagatcgc ccgccgacac cggttcgatc
      841 ccatagggtt cgagcagcgc gcgaatctcg cgcaccttgc ccgcgttatg gctcgcgatg
      901 acgagcttgc cgggggcgag cttgcgcgtc atcagcgccc cgtcgcccga tcctgcgccg
      961 cgaaaatctc gccgcagccg atgcgcgcga ggcggagcag gcgaagcagg ccctcttcgt
     1021 cataggtcgc gccctcggcg ctcgcctgca cttcgacgat ctggcccttg ccggtcagca
     1081 cgaaattgcc gtcggcctcg gcgaccgaat cctcgtcata gtcgaggtcg agcaccggcg
     1141 tgccccgata gatgccgcac gaaatcgcgc cgaccttgtc ctcgatcgga tcctcggtca
     1201 gcgtcttcgc cgcgagcagc ttgtcgaccg cgagccgcat cgcgacccac gcgcccgaaa
     1261 tcgacgcggt gcgcgtgccg ccgtcggcct ggatgacgtc gcagtcgatg atgatctgcc
     1321 gctcgccgag cttcttcata tcgacgacgg cgcgcaagct acggccgata agccgctgga
     1381 tttcctgcgt gcgccccgac tgcttgccct tcgccgcttc gcggctgccg cgcgtgtgcg
     1441 tcgcgcgggg cagcatgcca tattcggcgg tcacccagcc tgcgcccttg ccgcgcatcc
     1501 acgacggcac cttctcgtcg acgctcgcgg tcacgagcac cttggtattg ccgaagctca
     1561 cgagcacgct gccctcggcg tggatggtga actcggtttc gatggcgatg gggcgcatct
     1621 ggtcgggcgc gcggccggaa gggcgcatga actttccttt cgattgatga ttgcggtggc
     1681 ccttaggccg ccgtcctcat tcgctcaacc gcatcttgaa gaattcgagg atttcgtcgc
     1741 gcgcctgcac cgtcggctga cctacctcgt cgatgaggtg gagcgtgacg acgctgtgcg
     1801 ggttcttgta cggcgtgtcg gggtttgcgg cgctgtcggg cagcacgcgg ccgatgaagc
     1861 ggtcgccgag cgcatcttca taggccgcga agcgcgccgc cttgcaatat ttgtcgccct
     1921 cgaaacgata ggcgagcatg gtcaggtcct cggcctccat tcgtgccttc acttcggcga
     1981 gttcgtccgg ggcgatgtgc atgcccgccg gatcgttgag tggcagcgac ggctgcgcga
     2041 gcacgggcgc gagcaccgcg ggttcgagca tcatcgacag cgcgaaattg ccggtgaaac
     2101 acatgccgat cgcgccgacg cccttgccgc cgcattcctt atgcgccagc gccgcgagcg
     2161 cgcgcagcca ccgcgtcgcg ggcgagcttt cattcgcctg gaaggcacgg aactgccggc
     2221 tgatgcaccc gccgatcatc gtgaacagcg cacccggcat ccgcaccacc ccgccatcct
     2281 tgccgaagat atgcggcata tagacggtga aacccgcatc gcggacccag cgggcgaaac
     2341 gcgcgacatg cgggctgatg ccgggcattt cggtcatcac gacgaccgcg ggaccgctgc
     2401 ccatcgtgta gacgcggtgc ttgcggccga gcaggacgat gtcgcggtgg tcgaaatcat
     2461 cgagcgggtc gtcctgatta agcgggcgtg tcgtcatggt tttctccccg atggcaaggc
     2521 tagacaggag aatatccaat tgcactccta tttcgtcatt cccgtttgcg cgggaatgac
     2581 gaggttggcg ccaggcttgc ccctcgccct tcccgtccct aaatcctccc catgaccacg
     2641 ccgccgatca ccgaactcac cacgcgcgcg cgcgatgtgt tccggctggt ggtcgacgcc
     2701 tatctcgaga ccggacagcc ggtcgggtcg cgcaccctgt cgaagctcgc ggcgctgaac
     2761 ctgtcgcccg cctcgatccg caacgtgatg caggacctcg aggaatatgg cctgctcgcg
     2821 agcccgcaca ccagcgccgg ccgtctgccg accgaacagg gcctccgcct cttcgtcgac
     2881 gggatgatgc aggtcgccga accctcggca gaggaccgcg cgcagatcga ggcgagtctc
     2941 tccgatgccg gcccgatcga aagtgcgctc gcgcaggcga cctccgccct gtccggactg
     3001 tcggcgtgcg cgggcctcgt cctcgtcccc aagcacgagc gggtcttgaa acagctcgcc
     3061 ttcgtgccgc tgtcggcgaa ccagtcgctc gtcgtgctcg tcgcgggcga cgggacggtc
     3121 gaaaatcgcg tgatcgacat tccgcctggg ctcaaccctt cggcgctggt cgaggcgggc
     3181 aattatatct cggcgacgct gtcggggctt acactcaccg aggcgatggc gcgcgtgcgg
     3241 cgcgagatcg aggccgagcg catcgcgatc gaccgcgcgg cgcaggacct tgtcagccgc
     3301 ggccttgcga tctggtcgtc ggacggcgcc gaccgcccgg tgctgatcgt gcgcgggcaa
     3361 gcgaatctgc tcgacgagag cgcggtcggc gacctcgacc gcgtccgcca gcttctcgac
     3421 gaactcgaaa ccaagcagga catcgcgcaa ttgctcgaca gcgcgcgcga aggtgccgca
     3481 acccgcattt tcatcgggtc ggagaataaa ttattctcgc tttcgggctc gtccgtgata
     3541 gcggcgccct atcgcggatc ggacgggcgc gtcgtcggcg tggtcggggt catcggcccc
     3601 acacgcttga actatgcccg catcgttccc atggtggatt tcaccgcaca atcgctctcc
     3661 agactgatac gataggttta tgacgaacat cgaaaacgac acgcccgtcg acgacggcgc
     3721 gaataccgaa aacgagacca ccgagacggc cgcacccgaa ggcgcgaacg acgaagtcgc
     3781 gcagctcgcc gaacaggttg ccgcgctcca gcaggacctg ctctacgcgc gcgccgagac
     3841 gcagaatgtc acgcgccgca aggacaagga aatcgccgac gcgcatgcct atgcgtcaac
     3901 caaattcgcg cgcgacatat tgtcggtcgc cgacaatctc ggccgcgcgc tcgccgcgct
     3961 cagcgaagag cagcgctccg atgaaggcat caagccgctg attaccgggc tggaggccac
     4021 cgaacgcgaa ctgctcagcg tcttcgaacg caacggcatc acgcgcatcg ccgcgatcgg
     4081 cctgccgctc gacccgaacc agcatcaggc aatgctcgaa attccgagcg acaagacgcc
     4141 cggcacgatc gtgcaggaaa tgcaggcggg ctatatgatg aaggaccggc tgctgcgccc
     4201 cgcgatggtg ggcgtcgcga aggcggggta aagaaaaacc ctctcccctt ggggagaggg
     4261 atgcgcaagc ttggcggctt gccgcctagc tgaagctggg tgagggtctg aaacgtagcg
     4321 gcagagcgcc ctcacccgct gcgactagcc agcaagctgg caagtctcgc tgccctctcc
     4381 cccacaaggg gagagggttg aaatcacgcc gccttgcgca gcttcgcgag cttcttcagc
     4441 accatttcgc gcttcagccg cgacagatgg tcgatgaaga gaatgccttc gagatggtcg
     4501 tgctcatgct ggatgcacgt cgccatcagg ccggtcatgc gctcctgatg atgcttgccg
     4561 tcctcgtctt gccagtcgac ggtcacctcg gcggggcgcg tcacatcggc atattgctcg
     4621 gggaccgaca agcagccctc ctgatagacg ctatgttcct cgctctcgtc cgaaaagacg
     4681 ggattgatga agacgcgcgg ctcgcggatc accttcttgc cctcttcgtc gtcgggatcg
     4741 ggctcctgca ggtcgatgac gaggatgcgc ttcggcaccg cgacctggat cgcggcaagc
     4801 ccgatgccgg gggcgtcgta catcgtctcg aacatgtccg cgaccagctg cttcagctcg
     4861 gcgtcgaagg tctcgacagg cttcgaaatg acgcgcaacc ggggatccgg ggtctctatg
     4921 atgggtagga tggccatggg tggcaggtat gatcgggggg gcgtcgggtc aagggtcacg
     4981 ttgattgccg acagaaattt gccatatgga gcctaaagga gattcttgat gccaactcat
     5041 gacccgctta caccttccca gatcgcacta ttgaacgaac tcgcgggggc accactcact
     5101 attcgcgttg gggatatgtc gagtactcag aaaggccgcg atatccagtt tctagcacgg
     5161 gcaggctatc ttaacgccga acatggtcat acagcccaag aaactgtgaa ttctacgttc
     5221 gtctattcca ggaatggtaa gccttaccct tagcactaac ccaccggcct tcttgcccga
     5281 agcgcctgcg ccaaagtccc ctcgtcgaga taatcaagct cgccgcctac cggcaggcca
     5341 tgtgcaagct gcgtcagccg caccggatag ccttccagcc gctcggcgag ataatgcgcc
     5401 gtcgtctgac cctccagcgt cgcgttcatc gcgagcacga cctcgtcgat gccgcctgcg
     5461 gcgacgcgcg cgacgagggc gtcgatgctg aggtcctgcg ggcggatacc ttccagcgcc
     5521 gaaagccggc cgccgaggac atgatatttg ccggggaaga gccgcgactt gtcgagcgcc
     5581 cagaggtcgg agacttcctc gacgacgcac aggctgcgcg catcgcggcg cgggtcggcg
     5641 cagatcgcgc acggatcctg cgtgtcgaca ttgccgcaaa tgccgcaggt gacgagtcgt
     5701 tcggacaccg tctggagcgc cgcgagcagc ggtgcgaagc tgctctcgcg cttcttcatc
     5761 agatgcagca ccgcgcgccg cgccgagcgc gggccgagcc ccggcaggcg ggcgagttgc
     5821 tggacgaggg cttcgatttc ggtggaggcc atggagcggt ggtagtgggt gagcgatcgg
     5881 tgcacaatat cccgcctcgt caccccggac ttgatccggg gtcc
//
LOCUS       DATMSU010000380         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_73043, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000380 DATMSU010000000
VERSION     DATMSU010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73043"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..865)
                     /locus_tag="VN034_10225"
     CDS             complement(140..865)
                     /locus_tag="VN034_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWV61022.1"
                     /translation="MVVSAKIESAMVMAAGLGKRMRPLTATRPKPLVRVAGKALIDHS
                     LDRIEAAGIGHVVVNVHYLADALEAHLAAQKRKFSIAISDERDLLLETGGGMVKALPQ
                     LKGDPILIVNSDNIWTDGPQDSIRHLARHWDGDKMDALLLLIRQASATGHGGRGDFHM
                     DPAGKLSRRKPGHIAPFVYTGIQLISRRFLDDAPEGPFSTMVLWERAIATGRLYGLSH
                     MGQWFDVGTPASIAPTEAALTDG"
     gene            complement(856..1827)
                     /locus_tag="VN034_10230"
     CDS             complement(856..1827)
                     /locus_tag="VN034_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWV61023.1"
                     /translation="MIPPAHAPAFLAAHGWGDAQILPLAGDASFRRYFRVVDKGRQAV
                     LMDAPPPHEDPRPFIAVAEYLCGLGLNAPTIMGRDLDRGLLLIEDFGDVRLRETVDAA
                     LHKEAKYYAGVTDLLVHLHAQPPMVGLPVHGIAQWLDEVMLFTDWYCPAFGIEVDRDA
                     FRGAWEAALMPVETDGLPRVTVLRDFHAENIMLVEGREGIGHYGLLDFQDALVGHPAY
                     DLASVLEDARRDVTPAVEAAMLARYGEATGRDIENAYWALAAQRNTRILGVFVRLWKR
                     DDKPHYKSFQPRMWGLLERDLAHPALVPVRQWFDANVPASKRAEAWS"
     gene            complement(1824..>2086)
                     /locus_tag="VN034_10235"
     CDS             complement(1824..>2086)
                     /locus_tag="VN034_10235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWV61024.1"
                     /translation="VQPYAPPETDLPIAHVDLYRIEDSDELIELGLDDYLYDGALLIE
                     WPERLGGEGWPGALGLRISGEGDARVLTADVPPAWEARWPLR"
BASE COUNT          363 a          730 c          667 g          326 t
ORIGIN      
        1 ccgcgcgcgg ttgctcggca gcaggatcaa cccctgcgcg agcccgagcg caccgtcggc
       61 aaagcgcgcg atcagccccg ccgcgagcgc atcggcgaag gcccgctgga ccgggatgga
      121 aaaaagcgtg ggctttgcgt cagccatccg tcagcgcggc ttcggtcggc gcgatgctcg
      181 ccggggtgcc gacgtcgaac cactgcccca tgtgcgacag cccatagagc cgcccggtgg
      241 caatggcccg ttcccacaga accatcgtcg agaagggccc ttcgggcgca tcatcgagga
      301 accgccgcga aatgagctgg atcccggtat aaacgaaggg cgcgatatgc cccggcttgc
      361 ggcgcgacag cttgcctgcg gggtccatat ggaaatcacc gcgcccgccg tgccccgtcg
      421 cgctcgcttg ccggatcagc agcaacagcg cgtccatctt gtcgccgtcc caatggcgcg
      481 ccaagtgccg gatgctgtcc tgcggcccgt cggtccagat attgtcgctg ttgacgatca
      541 ggatcgggtc gcccttgagc tgcggcaacg ctttgaccat cccgccgccg gtttcgagca
      601 gcaggtcgcg ctcgtccgaa atcgcgatgc tgaacttgcg cttttgcgcc gcgagatgcg
      661 cttccaacgc gtcggcgaga taatggacgt tgacgacgac atggccgatg ccggccgcct
      721 cgatccggtc gaggctgtgg tcgatcagcg ccttgcccgc gacgcgcacc agcggcttcg
      781 gccgcgtcgc cgtcagcgga cgcatccgct tgccgagccc cgccgccatc accatagcgc
      841 tttcgatctt cgcactcacg accatgcctc cgcccgcttc gaagcgggga cattggcgtc
      901 gaaccattgc cgaaccggga caagcgccgg atgcgcgagg tcgcgttcga gcagtcccca
      961 catgcgcggc tggaaactct tgtagtgcgg cttgtcgtcg cgtttccaca gccgcacgaa
     1021 gacgccgagg atgcgtgtgt tgcgctgcgc cgcgagcgcc caataggcgt tttcgatatc
     1081 gcgccccgtc gcttcgccgt agcgcgcgag catcgccgcc tcgaccgcgg gcgtcacgtc
     1141 gcgccgcgcg tcttcgagca ccgaggcgag atcataggcc ggatggccga cgagcgcgtc
     1201 ctggaaatcg agcaggccat aatgcccgat cccttcccgc ccctcgacca gcatgatatt
     1261 ttcggcatgg aaatcgcgca gcacggtaac gcgcggcagg ccgtcggtct cgacgggcat
     1321 cagcgccgct tcccacgcac cgcggaaagc atcgcgatcg acctcgatcc cgaacgccgg
     1381 acaataccag tcggtgaaca gcatcacctc gtcgagccat tgcgcgatcc catggacggg
     1441 cagcccgacc atcggcggct gcgcgtggag atggacgagc aggtcggtca cccccgcata
     1501 atatttggct tccttgtgca gcgccgcatc gaccgtttcg cgcagccgca catcgccgaa
     1561 atcctcgatc agcagcagcc cccgatcgag gtcgcggccc atgatcgtcg gcgcattgag
     1621 ccccagcccg cacagatatt cggcgaccgc gatgaacggg cgcgggtcct cgtgcggcgg
     1681 cggcgcgtcc atcagcaccg cctgccggcc cttatcgacg acacggaaat agcggcggaa
     1741 ggacgcatcg cccgcgagcg ggagaatctg ggcatcgccc cagccatgcg cggcaagaaa
     1801 ggcgggcgca tgggcgggcg gaatcatcgg agcggccatc gcgcttccca agcgggcggc
     1861 acatcggctg tcaagacgcg cgcgtcgccc tcgcccgaaa tgcgcaggcc cagcgcgccc
     1921 ggccagcctt cgccgcccag ccgctcgggc cattcgatca gcaaggcgcc gtcgtaaaga
     1981 taatcgtcga gcccgagttc gatcagttcg tcactatcct cgatccggta cagatcgaca
     2041 tgcgcgatgg gcagatcggt ctcgggcggc gcatagggct ggacga
//
LOCUS       DATMSU010000381         9048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_139415,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000381 DATMSU010000000
VERSION     DATMSU010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139415"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VN034_10240"
     CDS             <1..712
                     /locus_tag="VN034_10240"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWV61025.1"
                     /translation="TVARIAAESRVPPAAHLTCVEASRAEIDEVARAYWEAGVRHIVA
                     LRGDVPGGEPYRPHPDGYANAIELVAGLKAIAPFDISVAAYPETHPDADCPQADLDNL
                     KRKLDAGATRAITQFFFSPDSFLRFRDAAAAAGIDAPIIPGILPVSNVSQTRRMADMC
                     GTAIPAWMVSLFEGLDDHPAARQLVAATIAAEMCRRLYAGGVRDFHFYTLNRAELSYA
                     ICHLLGLRPVSTAKDQAA"
     gene            709..1773
                     /locus_tag="VN034_10245"
     CDS             709..1773
                     /locus_tag="VN034_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HWV61026.1"
                     /translation="MTPNSIKGAREALTAAAKERILLTDGGWGTQIQLRKLVEADYAG
                     SLGLSHDQKGNNDILALTRTDVVTAIGEAYLAAGSDIVSTNTFSANRISQADYGAEHL
                     VADINHESARLGREAANKYEALDGRRRFIAGAVGPTNKTLSLSPDVNNPGYREIDFDE
                     LKDVYLEQVVALAEGGADFILIETIFDTLNAKAGIAATLEAEAKVGRELPLMISMTLT
                     DLSGRNLSGHTVEAFWYAVRHAKPLTIGLNCSFGASQLRPHVKVLSDIADALVMVYPN
                     AGLPNELGEYDEMPDTTAGLVREWAEHGQVNILGGCCGSTPAHIAAMAKAIEGLAPRH
                     IPDVPVRTRLAGLEPFTMAA"
     gene            1897..4521
                     /gene="metH"
                     /locus_tag="VN034_10250"
     CDS             1897..4521
                     /gene="metH"
                     /locus_tag="VN034_10250"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWV61027.1"
                     /translation="MTENSSASSSFVNIGERTNVTGSAAFKKLILADDYTAAVEVARQ
                     QVENGAQVIDVNMDEGLLDAEYAMTTFLKLIAAEPDIARIPVMIDSSKWDVIEAGLKC
                     VPGKPIVNSISMKEGEEQFLDHAKKCMAYGAAVVVMAFDEVGQADTKERKIEICERAY
                     KLLMTIGFPPEDIIFDPNVFAVATGLEEHDNYAVDFIEAVKEIRVRCPHVHFSGGLSN
                     LSFGFRGNETVRRAMHSVFLYYAIPAGLDMAIVNAGQLDVYDTIDPELRQAVEDVVLN
                     RKVEGEAESPTERLIALAERYKGSNPAQEKAAEEWRGWAVEKRLEHALVKGIDAYVVD
                     DTEEMRLLMPRPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLLPF
                     IEASKEPGAKGKGKVVMATVKGDVHDIGKNIVGVVLQCNGFEIVDLGVMVPWSKILEA
                     ANENDADMIGLSGLITPSLDEMVTVAEEMQRAGMTMPLLIGGATTSKVHTALRIDPAY
                     KGPVLHVLDASRAVGVATALVSDTGRDAYVQGYKDDYEHVRDVRAGKGQSVLHTLEEA
                     RANYYDAYLSDKPAPPLQPGLHRFDDWSLEDLRECIDWTPFFRAWELHGTYPSILDDE
                     VVGETARALKADADAMLDQLIAEKWLTARGVCALWPCARDGDSVTIHLAEEERHVTLP
                     FLRQQIKKSRDRANMCLADFIDPAGDWMGGFAVGLHGIEPHSERFRADKDDYSDILLK
                     ALADRFAEAFAERLHQHVRTELWGYAPGEQLTNEALIKEEYRGIRPAPGYPACPDHSL
                     KPILFDLLAAGENAGLVLTESFAMLPTAAVSGFYFGHPESQYFGVARIGSDQLEDYAA
                     RRGVDIETATRWLRPNLD"
     gene            4747..5700
                     /locus_tag="VN034_10255"
     CDS             4747..5700
                     /locus_tag="VN034_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HWV61028.1"
                     /translation="MFTSLLRRPMLPLLALPLLLAPMPLPAQTGGAPYSIEGRGFSRL
                     QDAVDAIGEGEGTIRIAPGYHRDCAVQTAGRIAFVAAEPGRAIFDGVTCEGKAALVLR
                     GDGARVDGIVFQNMRVPDGNGAGIRLETSDLDIVNAMFRDSEQGILTADDPEATLTID
                     RSTFSRLGRCDRGLSCAHSVYTGIYGRVAVTRSRFEKGSGGHYLKTRAAEVDVNDNSF
                     DDTQGQATNYMIDLPSGSVGRISNNLFVQGRDKENYSAFIAVAAEERKNPSRGLIIEG
                     NRASLPAGIDRRSVFVADWSGEALAIGANELGTGLTKFEKR"
     gene            complement(5830..6336)
                     /locus_tag="VN034_10260"
     CDS             complement(5830..6336)
                     /locus_tag="VN034_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HWV61029.1"
                     /translation="MSDDLYDDDDGQDAAPMEMLASYFAAHDWPHEMVGEDEIVATAQ
                     GSWTTYELRAVWRPDDGVIQLLAFPDIRVVEDKRGVAHEALALINEQLWLGHFELWSN
                     SGTILFRHGMLVGADAGLSLDLTETLIESAIDECERFYPVFQFVLWGGKTPAEALAAS
                     LIETRGEA"
     gene            complement(6465..6812)
                     /locus_tag="VN034_10265"
     CDS             complement(6465..6812)
                     /locus_tag="VN034_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HWV61030.1"
                     /translation="MQSENRFFDDLAKMVNGIAGTVAGAGREAEAAMRDRAKEFVGRM
                     DFVSREEFEAVKQMAATARAEAEALKARLDKLEGVAKPAAAPKAAAKPAAANQAAKPA
                     AKPAAKPKAAPKK"
     gene            complement(6861..7412)
                     /locus_tag="VN034_10270"
     CDS             complement(6861..7412)
                     /locus_tag="VN034_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HWV61031.1"
                     /translation="MTEIATQGQLRMSYLRWALVTVPAIVLIGSLMGLLSNSGYSNRW
                     FTALDLPAITPPGWVFSTVWPILYICLGLSLAMILHARGAKGRGFALLLFFVQLIANF
                     TWSPLFFGQHQVTTALYLIIFILMVTIATAFAFAPIRKAAAWLLVPYMVWLSFAAILN
                     FQIDQRNPDAETLVPAAASTQIR"
     gene            complement(7474..8214)
                     /locus_tag="VN034_10275"
     CDS             complement(7474..8214)
                     /locus_tag="VN034_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWV61032.1"
                     /translation="MAKQRADQLLVDRGLAESRTRAQALILAGLAFVGDRKIDKAGQQ
                     IAADAELSVKGRDHPWVSRGGIKLDHALQHLGWDVAGAIAIDVGSSTGGFTDVLLSRG
                     AARVYAVDSGTNQLAWKLRQDARVVVHEQTSARILTADHIPERVDLIVCDASFIALSK
                     VLPVPMGFAKPGARMIALIKPQFEAERHEVGKKGVVRDAAVHARVCAGVRDWLTDEDW
                     DVVDTLESPITGPEGNVEFLIAAVNRAA"
     gene            complement(8214..>9048)
                     /locus_tag="VN034_10280"
     CDS             complement(8214..>9048)
                     /locus_tag="VN034_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWV61033.1"
                     /translation="TTGTAGSTFSPAALAAFVATPCSGALLGAALGATLVLPAWAALP
                     IFGGLGLGLALPFLAIGFVPALRRRLPKPGPWMDRFRKWMALPMGLTTLALAWLLWRQ
                     LDSGEQLIWPILAVAMALVLLTHYGAIQRGDGRSWLLIASGLLLVVSLAGTVVEVAEA
                     ERTADGSGSTFSPDALAKARATGKPVFVYFTADWCLSCKANEAGAIEREAVQKAFDKA
                     GVVTLVGDWTNGDPVITRTLAEHGRNSVPLYLWYAPGAAKPEILPQILTPGMLADKAK
                     R"
BASE COUNT         1605 a         2915 c         2997 g         1531 t
ORIGIN      
        1 gaccgtcgcg cgcatcgccg ccgaaagccg cgtgccgccc gcggcgcacc tgacctgcgt
       61 cgaggcctcg cgcgccgaga tcgacgaggt cgcgcgcgct tattgggaag cgggcgtgcg
      121 ccatatcgtc gcgctgcgcg gtgacgttcc gggcggtgag ccttatcggc cgcatcctga
      181 tggttacgcc aatgcgatcg aactggtcgc ggggctgaag gcgatcgcgc cgttcgacat
      241 ttcggttgcc gcctatcccg aaacgcatcc cgacgccgat tgcccgcagg ccgacctcga
      301 caatctgaag cgcaagctcg acgccggcgc gacccgcgcg atcacgcaat tcttcttctc
      361 gcccgacagc ttcctgcgct ttcgcgacgc ggccgctgcg gcggggatcg acgcgccgat
      421 cattccgggc atcctgcccg tgtcgaacgt gtcgcagacg cgccgcatgg ccgatatgtg
      481 cggcaccgcg attccggcgt ggatggtgtc gctgttcgag gggctcgacg atcatcccgc
      541 cgcgcgccag ctcgtcgcgg cgacaatcgc cgccgaaatg tgccgccgtc tctatgcggg
      601 cggcgtgcgc gacttccatt tttacaccct caaccgcgcc gagctcagct atgcgatctg
      661 ccatctgctg gggctgcggc cggtttcgac cgcaaaggat caggcagcat gacccccaat
      721 tcgatcaagg gcgcacgcga agccctcacc gcggcggcaa aggagcgcat cctcctgacc
      781 gacggcggct ggggcaccca gatccagctc aggaagctgg tcgaagccga ctatgccggc
      841 tcgctcgggc tgtcgcacga ccagaagggc aacaacgaca tcctcgcgct gacgcgaacc
      901 gacgtcgtca ccgcgatcgg cgaagcctat ctggcggcgg ggtcggacat cgtgtcgacc
      961 aacaccttca gcgccaaccg gatcagccag gccgattatg gcgccgagca tctggtcgcc
     1021 gacatcaacc atgaaagcgc gcggctcggc cgcgaagccg ccaacaaata tgaggcgctc
     1081 gacggccgcc gccgcttcat cgcgggcgcg gtggggccga cgaacaagac gctctcgctg
     1141 tcgcccgacg tcaacaaccc cggctatcgc gagatcgact tcgacgagct gaaggacgtc
     1201 tatctcgaac aggtcgtcgc gcttgccgaa ggcggcgccg atttcatcct gatcgagacg
     1261 atcttcgata cattgaacgc caaggcgggc atcgccgcga cgctcgaagc cgaagcgaag
     1321 gtcggacgcg aattgccgct gatgatctcg atgacgctca ccgacctctc cggtcgcaac
     1381 ctgtcggggc acacggtcga ggccttctgg tatgcggtgc gccatgccaa gccgctcacc
     1441 atcgggctca actgctcgtt cggcgcgtcg cagctgcgcc cgcatgtgaa agtgctgtcc
     1501 gatatcgccg acgcgctggt gatggtctat cccaatgcgg ggcttcccaa cgaactcggc
     1561 gaatatgacg agatgcccga cacgacggcg gggctcgtgc gcgaatgggc cgagcatggg
     1621 caggtcaaca ttctcggcgg ctgctgcggg tcgacccccg cgcatatcgc ggcgatggcg
     1681 aaagcgatcg aaggtctcgc gccccggcat attcccgacg ttccggtgcg cacgcggctg
     1741 gcagggctgg agccgttcac gatggcggcc tagtccccct cccgcttgcg ggaggggtta
     1801 ggggagggcc tgtccttttc cgacgcgtcg cccgagaatt tgaaacagac cctcccccga
     1861 cccctcccgc aagcgggagg ggagaagaag atcccgatga ccgagaatag ttccgcctct
     1921 tccagcttcg tcaatatcgg cgagcgcacc aatgtcacgg gttccgcggc gttcaagaaa
     1981 ctgattctcg ccgacgatta taccgcggcg gtcgaggtcg cgcgccagca ggtcgaaaat
     2041 ggcgcgcagg tgatcgacgt caacatggac gagggtctgc tcgacgccga atatgcgatg
     2101 acaaccttcc tcaagctgat cgccgccgaa cccgatatcg cgcgcattcc ggtgatgatc
     2161 gacagctcga aatgggatgt gatcgaggcg gggctgaagt gcgtccccgg caagccgatc
     2221 gtcaattcga tcagcatgaa ggagggcgag gagcaattcc tcgatcatgc caagaagtgc
     2281 atggcctatg gcgccgcggt cgtcgtgatg gcgttcgacg aggtcgggca ggccgatacc
     2341 aaggagcgca agatcgagat ctgcgagcgc gcctacaagc tgctcatgac catcggcttt
     2401 ccgcccgagg acatcatctt cgatcccaat gtgttcgcgg tcgcgacggg gctcgaggag
     2461 catgacaatt atgcggtcga cttcatcgag gcggtcaagg aaatccgcgt ccgctgtcca
     2521 cacgtccatt tttcgggcgg gctgtcgaac ctgtccttcg gcttccgcgg caacgagacg
     2581 gtgcgccgcg cgatgcacag cgtcttcctc tattatgcga tccccgccgg gctcgacatg
     2641 gcgatcgtca acgcggggca actcgacgta tacgacacga tcgaccccga actgcggcag
     2701 gcggtcgagg atgtcgtgct caaccgcaag gtcgagggcg aggccgaaag cccgaccgaa
     2761 cggctgatcg cgctcgccga acgctataag ggcagcaacc ccgcgcagga aaaggcggcc
     2821 gaggaatggc gcggctgggc ggtcgaaaag cggctcgaac atgcgctggt caagggcatc
     2881 gacgcttatg tcgtcgacga taccgaagag atgcggctgc tgatgccgcg cccgatcgag
     2941 gtgatcgagg gtccgctgat ggacgggatg aacgtcgtcg gcgacctgtt cggttcgggc
     3001 aagatgttcc tgccgcaggt cgtcaaatcg gcgcgcgtga tgaagaaggc ggtcgcgcac
     3061 ctgctgccct tcatcgaggc ttcgaaggaa cccggcgcga agggcaaggg caaggtcgtg
     3121 atggcgaccg tcaagggcga cgtccacgac atcggcaaga atatcgtcgg cgtcgtgctc
     3181 cagtgcaacg ggttcgagat cgtcgacctt ggtgtgatgg tgccgtggag caagatcctc
     3241 gaggcggcga acgagaatga cgccgacatg atcggcctgt cgggcctgat caccccctcg
     3301 ctcgatgaga tggtgacggt cgccgaggag atgcagcgcg ccggcatgac gatgccgctg
     3361 ctgatcggcg gcgcgacgac gtcgaaggtc cacaccgcgc tgcgtatcga cccggcgtac
     3421 aagggcccgg tgctccatgt cctcgacgcg agccgtgcgg tcggcgtcgc gacggcgctg
     3481 gtcagtgaca ccgggcgcga cgcctatgtg cagggttaca aggacgatta tgagcatgtc
     3541 cgcgacgtgc gcgcgggcaa ggggcagagc gtgctgcata cgctcgaaga agcgcgggcc
     3601 aattattacg acgcctatct cagcgacaag cccgcaccgc cactgcaacc cgggctgcac
     3661 cgcttcgacg actggtcgct cgaagatttg cgcgaatgca tcgactggac gccctttttc
     3721 cgcgcgtggg aattgcacgg cacctatccg tcgatcctcg acgacgaggt ggtcggtgag
     3781 acggcgcgcg cgctcaaggc cgatgccgat gcgatgctcg accagctgat cgccgagaaa
     3841 tggctgacgg caaggggcgt ctgcgccttg tggccgtgcg cgcgcgacgg cgacagcgtc
     3901 acgatccacc tcgctgaaga ggagcgtcat gtgacgctcc ccttcctccg ccagcagatc
     3961 aagaagagcc gcgatcgcgc caatatgtgc ctcgccgatt tcatcgatcc agcgggcgac
     4021 tggatgggcg gcttcgcggt cggccttcac ggtatcgagc cgcattcgga gcgtttccgc
     4081 gccgacaagg acgattattc ggacattttg ctgaaggcgc tcgccgaccg tttcgccgag
     4141 gcgttcgccg agcggctgca ccagcatgtc cgcaccgaat tgtggggcta tgcgccgggc
     4201 gagcaactga ccaacgaggc attgatcaag gaggaatatc gcggcatccg gccggccccc
     4261 ggctatcccg cctgtcccga tcatagtctg aagccgatcc tgttcgatct gctcgccgcg
     4321 ggcgagaatg ccgggctggt gctcaccgaa agcttcgcga tgttgcccac cgcggcggtc
     4381 agcggcttct atttcggtca ccccgaaagc cagtatttcg gcgtcgcacg gatcgggagc
     4441 gatcagctcg aggattatgc cgcgcgccgc ggggtcgata tcgaaaccgc gacgcgctgg
     4501 ctgcggccca acctcgattg atcgcggctt aatttgggcc tcgtcaccct gaacttgttt
     4561 cagggtccat ggtcggccct ttacttcggc gccgcgctga agacgcgggc aggccatgga
     4621 tgctgaaaca agttcagcat gacgaaggtt taatttcctt cggcccgcct gtccgaaagc
     4681 gagacaggcg cgcaattaac cagcttcgcc aacgactaga cgcgtgaaaa cggctatgcg
     4741 cggatcatgt tcacctcgct ccttcgccgg cccatgctgc cgctgctcgc ccttccgctg
     4801 cttctggcgc cgatgccgct tcccgctcag acgggcggcg cgccctacag tatcgaaggc
     4861 cggggctttt cgcgcttgca ggacgcggtc gatgcgatcg gtgagggcga ggggacaatc
     4921 cgtatcgccc ctggctatca ccgcgactgc gcggtgcaga ccgcggggcg gatcgccttc
     4981 gtcgccgccg agccgggccg ggcgatcttc gacggcgtga cgtgcgaggg taaggcggca
     5041 ctcgtgctgc gcggcgatgg cgcgcgcgtc gacggcatcg tctttcagaa tatgcgcgtc
     5101 cccgacggca acggtgcggg cattcggctc gaaacgagcg atctcgacat tgtgaacgcg
     5161 atgttccgcg acagcgaaca gggcatcctc accgccgacg atccggaagc gacgctgacg
     5221 atcgaccgtt cgaccttttc gcgcctcggc cgctgcgacc gcggattgag ctgcgcgcac
     5281 agcgtctaca cgggcatcta cggccgcgtc gccgtcaccc gctcgcgttt cgaaaagggc
     5341 agcggcggcc attatctgaa gacacgcgcg gctgaggtcg acgtcaacga caacagcttc
     5401 gacgacacgc aggggcaggc gaccaattat atgatcgacc tcccctcggg atcggtcggg
     5461 cggatttcga acaatctgtt cgtgcagggc cgcgacaagg agaattattc ggctttcatc
     5521 gccgtcgcgg ccgaagagcg caagaatccg tcgcgcggcc tgatcatcga gggcaatcgc
     5581 gcgagcctgc ccgcgggcat cgatcgccgg tcggtgttcg tcgccgactg gagcggcgag
     5641 gcgctggcga tcggggcgaa cgagctgggc acggggctga cgaagttcga gaagaggtaa
     5701 ggcctacctc aaattccgtt tgccctgagc ttgtcgaagg gctgctcttt ctggcggcgt
     5761 cgaaaagaag aacggtcctt cgacaagctc aggacgaacg gaagttgggt aagtttggga
     5821 tcgattggcc taggcctcgc cgcgcgtctc gataagcgac gccgcgagcg cctcggcggg
     5881 ggtcttgccg ccccacagca caaactggaa cacaggatag aagcgctcgc attcgtcgat
     5941 cgcgctttcg atcagcgtct cggtcaggtc gagcgatagc cccgcatcgg cgccgacgag
     6001 catgccatgg cggaagagga tcgtgccgct gttcgaccag agctcgaaat gaccgagcca
     6061 gagctgctcg ttgatcagcg cgagcgcttc gtgcgccacc ccgcgcttgt cctcgacgac
     6121 gcggatgtcg ggaaaggcga ggagctggat caccccgtcg tcggggcgcc acaccgcgcg
     6181 cagctcatag gtcgtccagc tgccctgcgc ggtcgcgacg atctcatcct cgccgaccat
     6241 ctcgtgcggc cagtcgtgcg cggcgaaata ggacgccagc atttccatcg gcgccgcgtc
     6301 ctggccgtca tcgtcgtcgt atagatcgtc actcatagga gcgcctttag cggctgttcg
     6361 gccgggtggc gagtcagccg cacaaaaacg tcgcccgcga atgtgcgggc tgcctgcgcc
     6421 ctcatgccaa gcctatggcg gccccgcctg cgcggggacg acgctcattt cttgggggcg
     6481 gctttcggtt tcgccgcggg cttggcagcg ggtttcgcgg cctgatttgc cgcggcgggc
     6541 tttgctgcgg ccttcggggc agcggccggc ttcgcgacgc cttcgagctt gtcgaggcgc
     6601 gccttcagcg cttcggcttc ggcgcgtgcg gtggcggcca tctgcttcac cgcctcgaat
     6661 tcttcgcggc tgacgaaatc catgcggccg acgaattcct tcgcgcggtc gcgcatcgcg
     6721 gcttcggctt cgcggccggc gcccgcgacg gtcccggcaa tgccgttcac catcttggcg
     6781 aggtcgtcga aaaagcggtt ttcgctctgc atgtctgata ttccttcgtc ggggatagcc
     6841 cccagttgtc ccttgcgcct tcaccttatc tgggtgctgg cggcggcggg gacaagggtt
     6901 tcggcgtcgg gattgcgctg atcgatctgg aaattgagga tcgcggcaaa gctgagccag
     6961 accatatagg gcacgagcag ccatgcggcg gccttgcgga tcggcgcgaa ggcgaaggcg
     7021 gtcgcgatcg tgaccatcag gatgaagatg atcagataga gcgcggtcgt cacctgatgc
     7081 tggccgaaga agagcggcga ccaggtgaaa ttggcgatca gctggacgaa gaagagcagc
     7141 agggcgaagc cgcgcccctt ggcgccgcgc gcatggagga tcatcgcgag cgacaggccg
     7201 aggcagatat agaggatcgg ccagacggtg ctgaagaccc agcccggcgg cgtgatcgcg
     7261 ggcaggtcga gcgcggtgaa ccagcgattg ctataaccgc tgttcgacaa aagccccatc
     7321 aggctgccga tcagcacgat cgccggcacg gtgacgagcg cccagcgcaa ataggacatg
     7381 cgaagctggc cctgtgtcgc gatttcggtc atgtccgcaa tatcccccaa atgcgaatcc
     7441 gttgcatttg tgtcgcgatt gcgcggcgca gcgtcaagcg gctcgattca ccgccgcgat
     7501 cagaaattcg acattgcctt cggggccggt gatcgggctt tcgagcgtgt cgacgacgtc
     7561 ccagtcttcg tcggtcagcc agtcgcgcac gccggcgcac acgcgcgcgt ggacggcggc
     7621 gtcgcgcacg acgcccttct tgccgacctc gtggcgctcg gcctcgaact gcggcttgat
     7681 cagtgcgatc atgcgggccc cgggttttgc gaagcccatc ggcaccggca ggactttcga
     7741 cagcgcgatg aaactcgcgt cgcagacgat caggtcgacg cgctcgggaa tatggtcggc
     7801 ggtcaggata cgcgcgctcg tctgttcgtg gacgacgacg cgcgcgtcct gccgcagctt
     7861 ccatgcgagc tggttggtgc ccgaatcgac ggcatagacg cgcgccgcgc cgcggctcag
     7921 cagcacatcg gtgaagccgc cggtcgacga gccgacatcg atcgcgatcg cgcccgccac
     7981 gtcccagccg agatgctgga gcgcatggtc gagcttgatc ccgccgcgcg acacccatgg
     8041 atggtcgcgg ccctttacgc tgagctcggc gtccgcggcg atctgctgcc ccgccttgtc
     8101 gatcttgcgg tcaccgacga aagcgagccc ggcgaggatc agcgcctgcg cgcgcgtccg
     8161 gctttcggcg agaccgcggt cgacaagcaa ctggtcggca cgctgtttcg ccatcagcgc
     8221 ttcgccttgt cggcgagcat tcccggcgtc aggatctgcg gcaggatttc gggcttcgcg
     8281 gcgcccggcg cataccaaag gtagagcggc acgctgttgc ggccatgctc ggcgagcgtg
     8341 cgcgtgataa ccggatcgcc gttggtccag tcgccgacga gcgtcacgac gcccgccttg
     8401 tcgaacgcct tttgcaccgc ctcgcgttcg atcgcgccgg cttcgttcgc cttgcacgac
     8461 aggcaccagt cggcggtgaa atagacgaag acgggcttgc cggtcgcgcg cgctttggcg
     8521 agcgcgtcgg gcgagaaggt cgagcccgag ccatcggcgg tgcgttccgc ttcggcaacc
     8581 tcgaccaccg tgcccgcaag gctgacgacg aggagcaggc ccgacgcgat caacagccac
     8641 gaccgcccgt cgccgcgctg gatcgcgccg taatgcgtca ggaggacaag cgccatcgcg
     8701 accgcgagga tcggccagat caactgttcg ccgctgtcga gctggcgcca cagcaaccat
     8761 gccagcgcga gcgtggtgag ccccatcggc agcgccatcc atttgcggaa ccggtccatc
     8821 cacgggcccg gcttgggcag gcggcgccgc aaggctggga cgaaaccgat cgccaggaag
     8881 ggcagggcaa gccccagccc aagcccgccg aagatcggca gcgccgccca cgccggcagc
     8941 accaaagtcg cgccgagcgc cgcgccaagc agcgccccgc tgcacggggt ggcgacgaag
     9001 gcggcgagcg cggcggggga gaaggttgaa ccggcggttc cggtcgta
//
LOCUS       DATMSU010000382         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_120675,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000382 DATMSU010000000
VERSION     DATMSU010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120675"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VN034_10285"
     CDS             <1..1260
                     /locus_tag="VN034_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWV61034.1"
                     /translation="VAAVVRLCAGAKVALVPQGGNSGMVGGATPDASGDQLLLSLRRM
                     NRVRQVDEAARLAVAEAGVILETFHHDVLAHGLRFPLTLGGKGSATIGGLVSTNAGGT
                     QVLRHGTMRALVAGIEAVLPDGSIFDGLAPLKKDNRGYDLRHLLCGAEGTLGIVTAAC
                     LHLVPAAAARRTAWIGVDSPETALLLLRRLDAAIGDALEGFELIPQSCLDAVLRHIPQ
                     TRAPLSAAQPWHVLLELAGDSDEALGETLDPELAAAIDAGLIRDVVIAKNERESDDFW
                     RLRDSISEAERAEGPALQHDISVPVDLMPAFIAENPRRLDRAFPGIGVLSFGHLGDGN
                     VHHHVQPPAVVDGALWLAEHGAAVSRLVYSHVVELGGSISAEHGIGQMKRDILAELDS
                     PARLSALRGVKAGLDPAGILNPGKLIA"
     gene            1374..2153
                     /locus_tag="VN034_10290"
     CDS             1374..2153
                     /locus_tag="VN034_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HWV61035.1"
                     /translation="MATAPAPANLPLFYKDLVPLSSVDHADFRARPLDSAEFLVGQHA
                     IPLTSDEFVSACRFFPIVFSAGNNPVPLALMGLNEGINTFVDDEGKLINPVYVPAYIR
                     RYPFLLAKLQPNSEELSLCFDPTSGALGKFDEGDVLFVDGQPSDQVHAVLEFCKNFEE
                     AGQRTGMFMDELKKADLLMDGEVSIQQEGNDKPYVYRGFQMVDENKLRELRGDVLRKL
                     MQNGILGLIFAHLFSLQLMREVFAEQVKSGKMPLNTELPAV"
     gene            complement(2240..>2572)
                     /locus_tag="VN034_10295"
     CDS             complement(2240..>2572)
                     /locus_tag="VN034_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1674 domain-containing protein"
                     /protein_id="HWV61036.1"
                     /translation="GAMPGGSASPGDRRRRGERRSAMTGNGVVGVAHEKSYIAGMMND
                     NSPNATIGGTPRAVKRPAHVKAPEGWTNSAVPAPRPIHEDKAEDQPGGRNPVRYGDWE
                     LKGVAVDF"
BASE COUNT          404 a          892 c          819 g          457 t
ORIGIN      
        1 gtggccgcgg tcgtccggct gtgcgccggc gcgaaggtcg cgctggttcc gcagggcggc
       61 aacagcggca tggtcggcgg cgctaccccc gatgccagtg gcgaccagct gctcttgagc
      121 ctgcggcgga tgaaccgagt acgccaggtc gacgaggcgg cccggctcgc cgtcgccgag
      181 gcgggggtga tcctcgaaac cttccatcac gatgttctgg cccacggcct gcgctttccg
      241 ctgaccctcg gcggcaaggg gtcggcgacg atcggcggcc tcgtgtcgac gaacgcgggg
      301 gggacgcagg tgctgcgcca cgggacgatg cgcgcgctgg tcgcggggat agaagcggtg
      361 ctgcccgacg gcagcatctt cgacgggctc gcgccgctca agaaggacaa tcgcggctat
      421 gatctgcggc acctcttgtg cggcgccgag gggacattgg gcatcgtgac cgccgcctgc
      481 ctgcatctcg tccccgcggc ggcggcgcgg cgcaccgcgt ggatcggcgt cgattcgccc
      541 gaaaccgcgc tcctgctgct gcgccgtctc gacgcggcga tcggcgatgc gctcgagggt
      601 ttcgagctga tcccgcaaag ctgcctcgac gcggtgctgc gccatattcc acagacgcgc
      661 gcgccgcttt cggccgcgca gccgtggcat gtgctgctcg aactggcggg cgacagcgac
      721 gaggcactgg gcgagacgct cgatcctgag ctcgccgcgg cgatcgacgc cggcctgatc
      781 cgcgatgtcg tgatcgccaa aaacgagcgc gaaagcgacg atttctggcg gctgcgcgat
      841 tctatttccg aagccgaacg cgccgaaggc cccgcgctgc aacatgacat cagcgttccg
      901 gtcgacctga tgcccgcttt catcgccgaa aacccgcgcc ggctggatcg cgcctttccg
      961 ggcatcggcg tgctctcctt cgggcatctc ggcgacggca acgtccatca tcacgtccag
     1021 ccgcccgccg tggtggatgg cgctctatgg ctcgccgaac atggcgcggc ggtcagccgg
     1081 ctcgtctatt cgcatgtcgt cgagctgggc ggatcgattt cggccgaaca tggcatcggt
     1141 cagatgaagc gcgacatatt ggccgaactc gacagcccgg cgcgactgtc ggcgctccgc
     1201 ggcgtcaagg ccggcctcga tcccgcgggc atcctcaatc ccggcaagct catcgcctga
     1261 cgcctcgcgc tgcccgcgcc ccgcgcacat cgcccccttg cgtctcaggg cgcggaccaa
     1321 taaggcgctg tatctttttc gtcccgccga tgggaaccta ccggagttga aacatggcca
     1381 ctgcgcccgc acctgccaat ctgccgctgt tctacaaaga cctcgttccg ctttcgagcg
     1441 tcgatcacgc cgatttccgc gcccgcccgc tcgacagcgc cgaattcctg gttggccagc
     1501 atgcgatccc gctgacgtcg gacgaattcg tgtcggcctg ccgctttttc ccgatcgtct
     1561 tttcggccgg caacaatccc gttccgctcg cgctgatggg cctcaatgaa ggcatcaaca
     1621 ccttcgtcga tgacgaaggc aagctgatca atccggtcta tgtcccggcc tatatccgcc
     1681 gctacccctt cctgctcgcc aagctgcagc ccaattcgga agaattgtcg ctgtgcttcg
     1741 acccgacctc gggcgcgctc ggcaagttcg acgaaggcga cgtgctgttc gtcgacggcc
     1801 agccgtcgga ccaggtccac gccgtgctcg aattctgcaa gaatttcgag gaagccggcc
     1861 aacgcaccgg catgttcatg gacgagctga aaaaggccga cctgctgatg gacggcgaag
     1921 tctcgatcca gcaggaaggc aatgacaagc cctatgtcta tcgcggcttc cagatggtcg
     1981 acgagaacaa gctgcgcgaa ctgcgcggcg acgtgctgcg caagctgatg cagaacggca
     2041 tcctcggcct gatcttcgcg cacctcttct cgctgcagct gatgcgcgaa gtgttcgccg
     2101 aacaggtcaa gagcggcaag atgccgctga acaccgaact gcccgccgtc taaaggcgga
     2161 taaacacaag gccgctcccg ctcgatacgg gggcggcctt ttgcgtttgg cgccgccgcc
     2221 cggcgcatgt catcgcagct cagaagtcga ctgcgacgcc tttgagctcc cagtcgccat
     2281 agcgcaccgg gttgcgcccg ccgggctgat cctcggcctt gtcctcatgg atcggccggg
     2341 gcgcggggac cgcgctgttc gtccagcctt cgggcgcctt cacatgcgcg gggcgcttta
     2401 cagcgcgcgg cgttccgccg atggtcgcgt tcgggctgtt gtcgttcatc atgcccgcaa
     2461 tataggattt ttcatgcgcc acgccaacca cgccgttccc ggtcatggct gatcgtcgct
     2521 ccccgcgccg ccgccggtcg ccgggcgagg ccgatccccc cggcatggcg cc
//
LOCUS       DATMSU010000383         4450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_25337, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000383 DATMSU010000000
VERSION     DATMSU010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25337"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1144)
                     /gene="secF"
                     /locus_tag="VN034_10300"
     CDS             complement(164..1144)
                     /gene="secF"
                     /locus_tag="VN034_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWV61037.1"
                     /translation="MRLLKLVPDNTNIDFLRWRKLASFMSFFLVAVSIALVAVKGLNL
                     GVDFVGGQSVRVEFTQTMPSIDEIREKVGDIGLGEATIQQFGSDKAVSIRTALPDGDK
                     AAAERAGQQLVSGIKAAFPTAKTGSVETVSGKVSEELIRTGAISLGLAILGISIYIWI
                     RFEWQFGVGALGRLFHEVSLTFGLFAVTQMQFDLNSVAALLTIVGYSLNDTIVVYDRI
                     RENLKKYRKMDIVPLLNLSINETLSRTVMTTVAMVLALGMLLIFGPDVIFGFTAAMLF
                     GVFIGGYSSVLMSTPVLVWLKVGPDSFVPRTTSGMEGGERIERKDDGAVV"
     gene            complement(1160..2773)
                     /gene="secD"
                     /locus_tag="VN034_10305"
     CDS             complement(1160..2773)
                     /gene="secD"
                     /locus_tag="VN034_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWV61038.1"
                     /translation="MLDFPRWKTIGISIILLLGILFSIPSFLPEPTRESLPSVLQAKV
                     NLGLDLAGGSHLLLEADIADLRKTQLTNMEKTVRTAMRAESGPDDDIAIGELSNAGGR
                     ISFLVRDQAQLDEARERLFSETQGAGLTGQRDWSINVIDSQRIVMTPTSAGQTQAVAN
                     AMDTARDIIDKRVNALGTREPTIIREGNDRVVVQVPGLEDPAALKELIGKTARLEFRM
                     VDTNADPAEAAAGRVPVGSEIVPYAEGEGSGAPFEVLRRQVMISGEQLINAQQSYDPQ
                     SNQPVVSIRFDSGGSNTFAKVTAQNVGKRFAMVLDGRVLSAPSINEPILGGSAQISGS
                     FSVASANALAISLRSGALPVKMTVVEERTVTPELGADSIRKGAIAGIVATVSVLVLML
                     VVYGRFGVYACIGLIFNVFLIIAIMAAFNATLTLPGIAGFVLTIGAAVDANVLINERI
                     REELQRGRKVFQAIELGYSEASRAIFDANVTNVIAASLMFWFGSGPIKGFAVVLTIGI
                     VTSVFTAVTVTRMFVAHWLRTKRPTSINI"
     gene            complement(2829..3158)
                     /gene="yajC"
                     /locus_tag="VN034_10310"
     CDS             complement(2829..3158)
                     /gene="yajC"
                     /locus_tag="VN034_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HWV61039.1"
                     /translation="MSTQLLLTQAAGSGGGAAGFLMLVPYLLIFAVFWFFLIRPQQVR
                     AKEHRAKVAAVKPRDQVVTGGGIVGKITRVDDDFVDVEIAQGVKIKVVKSTLADVLQP
                     GGKPAND"
     gene            3413..3489
                     /locus_tag="VN034_10315"
     tRNA            3413..3489
                     /locus_tag="VN034_10315"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3447..3449,aa:Pro,seq:ggg)
     gene            3781..>4450
                     /locus_tag="VN034_10320"
     CDS             3781..>4450
                     /locus_tag="VN034_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HWV61040.1"
                     /translation="MEFAPEIFAFLIAVAFLAGTIDAMAGGGGLLTIPALMAAGVPPV
                     SALATNKLQSTIGTGSAFLTFWRAGHVDLRRFAWPAIGAFAGSVLGATAVQYVGSDFL
                     AAFVPILLIAMGLYFLLAPPMSSVDRHARTGPLGLTLIVAALGFYDGFFGPGTGSFLT
                     LTLVALGGLGLVRAIGNTKFLNLATNIAGLLAMIVGGHVLWLLGLSMATANVAGNQLG
                     ARLAI"
BASE COUNT          783 a         1502 c         1385 g          780 t
ORIGIN      
        1 ccgctcccgc cgacgtcgca tccagcctcg gcggccgctt cgactgggat cgcaacggcc
       61 gcgaactcgc cgaccatctc cgccgctgcg ccgggcgcta gatgcggcaa cgccggcccg
      121 ctcaaaaagc ggaccggcgc ccatgttcac aaattgaagt ccctcagacg accgcgccgt
      181 cgtccttgcg ttcgatccgt tcgccgcctt ccatgcccga cgtcgtgcgc ggcacgaaac
      241 tgtccgggcc gaccttcagc cacaccagaa ccggcgtcga catcagcacg gaggaatagc
      301 cgccgatgaa cacgccgaac agcatcgccg cggtgaagcc gaaaatcacg tccgggccga
      361 agatcagcag catgccgagc gccagcacca tcgccacggt cgtcatcacc gtgcgtgaca
      421 gcgtctcgtt gatgctgaga ttgagcagcg gaacgatgtc catcttgcga tattttttca
      481 gattctcgcg aatacggtca tacacgacga tcgtatcgtt gagcgaatag ccgacgatcg
      541 tcagcagtgc agcgacgctg ttgaggtcga actgcatctg cgtcacggcg aacaggccga
      601 atgtcagcga aacctcatga aacaggcggc cgagcgcacc gacaccgaac tgccattcga
      661 agcgtatcca gatataaatg gaaatgccga ggatcgcgag acccaagctg atggcgccgg
      721 ttcgtatcag ctcctcggac accttgcccg agacggtttc gaccgagccc gtcttcgcgg
      781 tcggaaacgc cgccttgatc cccgatacga gctgctgccc cgcgcgttcg gccgccgcct
      841 tgtcgccatc gggcagcgcg gtgcggatcg agaccgcctt gtccgatccg aactgctgga
      901 tcgtcgcttc gcccaggccg atgtcgccga ccttttcgcg gatttcgtcg atcgatggca
      961 tggtctgcgt gaattcgacg cggaccgact ggccgcccac gaaatcgacg ccgaggttca
     1021 atcccttcac cgcgacgagc gcaatcgaca ccgccacgag gaaaaagctc atgaacgagg
     1081 cgagctttcg ccagcgcaga aagtcgatat tggtgttgtc ggggacgagt ttcagcaagc
     1141 gcatggtttc gctcccttct taaatattga tcgacgtcgg acgcttggtc cgcagccaat
     1201 gggcgacgaa catgcgcgtg acggtgacgg cggtgaagac gctggtcacg atgccgatgg
     1261 tcagcaccac ggcaaagccc ttgatcgggc ccgagccgaa ccagaacatc agcgacgccg
     1321 cgatgacgtt ggtcacgttc gcgtcgaaaa tcgcgcggct cgcctcggaa taaccgagtt
     1381 cgatcgcctg gaacaccttg cgcccgcgct gaagctcttc gcgtattcgc tcgttgatca
     1441 gaacgttcgc atcgaccgcc gcgccgatcg tcagcacgaa acccgcgata cccggcaggg
     1501 tcagcgtcgc gttgaacgcc gccatgatcg cgatgatcag gaagacgttg aagatgaggc
     1561 cgatacaggc atagacgccg aagcgaccat aaacgacgag catcagcacg agcaccgaaa
     1621 ccgtggcgac gatgcccgcg atcgcgccct tgcggatcga atcggcgccg agttcggggg
     1681 tcacggtgcg ctcttcgaca acggtcatct tcaccggcag cgcgcccgag cgcagcgaga
     1741 tcgccagcgc attggcgctc gcgacgctga agctgcccga aatctgcgcg ctgccgccca
     1801 ggatcggttc gttgatcgat ggcgccgaca gcaccctgcc gtcgagcacc atcgcgaatc
     1861 ttttgccgac attctgcgcg gtcaccttgg caaaggtgtt cgatccgccc gaatcgaacc
     1921 ggatcgagac gaccggctga ttgctctgcg gatcatagct ttgctgcgcg ttgatcagct
     1981 gttcgccgct gatcatcacc tggcggcgca acacttcgaa cggtgccccg ctcccctcgc
     2041 cctcggcata ggggacgatt tcgctgccca ccggcacgcg gcccgcggcg gcctcggcgg
     2101 gatcggcatt ggtgtcgacc atacggaatt cgaggcgggc ggtcttgccg atcagttcct
     2161 tcagcgccgc cggatcctcg agccccggga cctgcacgac gacgcggtca ttgccttcgc
     2221 ggatgatcgt cggctcgcgc gtgccgagcg cgttgacgcg cttgtcgatg atgtcgcgcg
     2281 cggtatccat cgcgttcgcc accgcctgcg tctgccccgc actcgtcggc gtcatcacga
     2341 tccgctgcga atcgatgacg ttgatgctcc agtcgcgctg cccggtcagc ccggcgccct
     2401 gcgtctcgct gaacagccgc tcgcgcgcct cgtcgagctg cgcctggtcg cggacgagga
     2461 agctgatccg cccgcccgcg ttcgacagct cgccgatcgc gatgtcgtca tcggggccgc
     2521 tttcggcgcg catcgcggtg cgcaccgtct tttccatgtt ggtgagctgc gtcttcctga
     2581 ggtcggcgat gtcggcctcg agcagcaagt ggctaccacc ggcaaggtcg aggcccagat
     2641 tgaccttcgc ctgcaggacc gacggcaggc tttcgcgcgt cggttcgggc aggaagctgg
     2701 ggatggaaaa gaggataccg agcagcagga tgatgctgat gccgatggtc ttccagcgcg
     2761 ggaaatcgag catggtctag aaatccgata gcaagtgcgg gggccggggg agagccagcc
     2821 ccaccgcgtc agtcgttggc gggcttgcca ccgggctgga ggacgtcggc cagggtcgac
     2881 ttgacgacct tgatcttcac gccctgtgcg atttcaacat cgacgaaatc atcgtcgacg
     2941 cgcgtgatct tgccgacgat gccgccgccc gtcaccacct ggtcgcgcgg cttgaccgcc
     3001 gcgaccttgg cgcgatgttc cttcgcgcgg acctgctgcg ggcggatcag gaagaaccag
     3061 aacaccgcga agatcagcag ataggggacc agcatcagga aaccggcagc cccgccgccc
     3121 gatccggccg cctgggtcag aagcaattgc gtcgacatgg atgaaaaact ttcgttgcag
     3181 gcagcgcccg atcccctccg gaacgagcgc gcaatatggc gcggcgccta acatgcaggg
     3241 gccgtgcgcg caaccggtcg tgcgcgcccc tcccccgcgg caagcgagcc cgaaatgggg
     3301 tccgcggcgg caagatcaag cccgcagcat cgatcgggcc gaaaagccgc catcgctgct
     3361 tgcatccccc gccaaggcgc gctaggggcc tcgcctgccc aatcgggccg gtcgggacgt
     3421 agcgcagcct ggtagcgcat cacactgggg gtgtgggggt cggaggttcg aatcctctcg
     3481 tcccgaccaa ttttaccggc aggaatagca agttgtgcgg gtggcgagcg gacgatgccc
     3541 caaacccctc ctcttcagag gaggggcagt gagacttggt ccggcgcagc cgggccttta
     3601 gtcgaacggg gtggtcgtgg cgggacgcgc acgccgggcc gagaccacca ccccgctgcg
     3661 actaacgaac aagttcgtaa gtctcgctac ccctcctctg aagaggaggg gctgacgtat
     3721 attttccgat gacccttccc ccgcttgagc attgcgatac ggcgcactag gagcgcccgc
     3781 atggaatttg ctcccgaaat cttcgccttc ctgatcgcgg tcgcttttct cgcggggacg
     3841 atcgacgcga tggcgggcgg cggcggcctc ctcacgattc cggcgctgat ggccgcgggc
     3901 gtgccgcccg tgtcggcact cgcgacgaac aagctgcaaa gcacgatcgg caccggatcg
     3961 gccttcctga ccttctggcg cgcggggcat gtcgacctcc gccgcttcgc ctggcccgcg
     4021 attggcgcct ttgccggctc ggtgctcggc gcgacggcgg tccaatatgt cggctcggat
     4081 tttctcgccg ccttcgtgcc gatcctcctg atcgcgatgg gcctctattt cctgctcgcg
     4141 ccgccgatga gcagcgtcga tcgccacgcg cgcaccggcc cgctcggcct caccctcatc
     4201 gtcgccgcgc tcggcttcta cgacggcttc ttcggcccgg gcaccggctc gttcctgaca
     4261 ctcaccctcg tcgcgctcgg cgggctcggg ctcgtccgcg cgatcggcaa cacgaagttt
     4321 ctcaacctcg cgaccaacat cgcgggcctg ctcgcgatga tcgtcggcgg ccacgtcctc
     4381 tggctgctcg gcctgtcgat ggccaccgcc aacgtcgcgg gcaatcagct aggcgcgcgg
     4441 ctcgcgatcc
//
LOCUS       DATMSU010000384         3935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_177212,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000384 DATMSU010000000
VERSION     DATMSU010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177212"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VN034_10325"
     CDS             complement(<1..866)
                     /locus_tag="VN034_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV61041.1"
                     /translation="MSFDQIRLDRHEGIALLTLHRPDRMNAFTTEMMLEIVAALDECD
                     ADDGVRAVIFTGAGDRAYCAGADLGQGAATFDYDKRTDKLAILPEGMSASPVAEDGTI
                     DWSHPLIRDSGGRVSMRIFDCKKPVLGAINGAAVGIGATMTLSMDARLASDTARYGFV
                     FARRGIVPEAASSWFLPRLVGIQNAVDWCYSGRLIPAQEAHEKGLVQSVHAPGDLVDA
                     AIAKARELTEHSAPVSVALTRHMLWRMLGAINGAAVGIGATMTLSMDARLASETARYG
                     FVFARLGIVPEAA"
     gene            1143..1922
                     /gene="purC"
                     /locus_tag="VN034_10330"
     CDS             1143..1922
                     /gene="purC"
                     /locus_tag="VN034_10330"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWV61042.1"
                     /translation="MARRRQIYEGKAKILYEGPEPGTLIQYFKDDATAFNAQKRGTIN
                     GKGVLNNRISEHVFTLLGNIGVPTHFIRRLNMREQLIRQVEIVPIEVIVRNVAAGTLS
                     KRLGIEEGTQLPRTLIEYCYKDDALGDPLVAEEHIACFNWCSQDELHDIQDMAIRIND
                     FMSGMFAAVGIRLVDFKLEFGRLYEGDFSRIILADEISPDGCRLWDMTTNEKLDKDRF
                     RRDLGGEVEAYQEVARRLGLMPEGGENAVLDLESHRKKKGS"
     gene            complement(2070..>3935)
                     /locus_tag="VN034_10335"
     CDS             complement(2070..>3935)
                     /locus_tag="VN034_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoxygenase family protein"
                     /protein_id="HWV61043.1"
                     /translation="LAAARTVYAWTTDVPTLPGVPLATNVPKNDEPTIAWFVVLIGVG
                     LGIVRNALTVKLGGVDKGELDSPRADYEAALAECDAIEASAAKIAAEHGVHTGGNIFE
                     RMIGDVEQAVAAAERDAHVALLKGYKARLEDLMKVEEALGLGSKTVRSIEAYRALFAT
                     LPVPGVSYMFQDDAEFARLRVQGPNCMLIAAVGTALPSHFPLRAAQYAAVVNGDTLEA
                     ALADGRLFMLDYQPLEVLDPGTYGSLAKYAWQPMALFAVPPGGSSLVPVAIQCGQDPA
                     DYPIFTPSPAADKLWGWEMAKLVVEVADGNYHELFTHLARTHLVIEAFAVATHRHLAE
                     AHPIWALLVPHFEGTLFINEAAATSLIAANGPIDHIFAGTIASSQAAAVDARLAFDFH
                     GKMPHADFAARGVGVDSALADYPYRDDALLVWDAIHEWARQYVDLYYAHDADLVADTE
                     LSAWAACLAGEAKIKGFGPVTTRKQLADVCAMVMFTASAQHAAVNFPQKDIMAFAPAV
                     TGAGWQAAPNGQRGHDKAGWLAMMPPMALALEQLNVLELLGSVHYRPLGDYRSNAFPY
                     PKWFQDPRVTAAEGPLVWFQAALKGVEAEIVQRNAERMQPYPYLQPSLIPTSINI"
BASE COUNT          685 a         1340 c         1275 g          635 t
ORIGIN      
        1 gccgcttcgg ggacgatccc gagccgcgcg aacacgaagc cgtagcgcgc ggtctcgctc
       61 gcgaggcgcg catccatcga caacgtcatc gtggcgccga tgccgaccgc ggcgccgttg
      121 atcgcgccga gcatccgcca cagcatgtgg cgcgtcagcg cgaccgacac cggcgcgcta
      181 tgctcggtca gctcgcgcgc cttggcgatc gcggcgtcaa cgaggtcgcc gggggcgtgg
      241 accgactgga cgaggccttt ctcatgcgct tcctgcgcgg ggatcagccg ccccgaatag
      301 caccagtcga cggcattctg gatgccgacc agacgcggca ggaaccagct cgacgcggct
      361 tcggggacga tcccgcgccg cgcgaagaca aagccgtagc gcgcggtatc gctcgcgagc
      421 cgcgcgtcca tcgacagcgt catcgtcgcg ccgatgccga ccgcggcgcc gttgatcgcc
      481 cctaatacgg gcttcttgca gtcgaagatg cgcatcgaga cgcgcccgcc cgaatcgcgg
      541 atcagcgggt gcgaccagtc gatcgtgcca tcctcggcga cggggctcgc cgacatcccc
      601 tcgggcagga tcgccagctt gtcggtgcgc ttgtcgtaat cgaaggtcgc ggcgccctgg
      661 ccgagatccg cccccgcgca ataggcgcgg tcgcccgccc ccgtgaagat caccgcgcgc
      721 accccgtcgt cggcgtcgca ttcgtcgagc gcggcgacga tctccagcat catctcggtg
      781 gtgaaggcgt tcatccggtc ggggcggtgc agcgtcagca gcgcgatgcc ttcgtggcgg
      841 tcgaggcgaa tctggtcgaa gctcatgtct ctctccctaa ggtcgcggtc atggttgcaa
      901 gcggtgccgg caatggcaag ccctttacgt aaacgtaaac tcatcgtcgc ccccgcgaag
      961 gcgggggccg ctggaaggac ggagtgcggc cgacaacggc ccccgccttc gcgggggcga
     1021 cgtgactggt gacgcgcccg acctttgtcg ctataggcgc ccccgtcgcc ccggtgccgc
     1081 gcctcgtcca tgagtcgttt ccgccggggt cattcatttg gttccgacag aaagtcctgc
     1141 ccatggcccg ccgccgccag atctacgaag gcaaagccaa gatcctttac gaaggccccg
     1201 aaccgggcac gctcatccaa tatttcaagg atgacgcgac cgcgttcaac gcgcagaagc
     1261 gcgggacgat caacggcaag ggcgtgctca acaaccggat ttcggagcat gtcttcaccc
     1321 tgctcggcaa tatcggcgtg ccgacgcact tcatccgccg cctcaacatg cgcgaacagc
     1381 tgatccgtca ggtcgagatc gtgccgatcg aggtgatcgt gcgcaacgtc gccgcgggaa
     1441 cgctgtcgaa gcggctcggc atcgaggagg ggacgcagct tccccgcacg ctcatcgaat
     1501 attgctacaa ggacgacgcg ctcggcgatc cgctcgtcgc cgaggagcat atcgcctgct
     1561 tcaactggtg cagccaggac gagcttcacg acattcagga catggcgatc cgcatcaacg
     1621 acttcatgag cggcatgttc gccgcggtcg gcatccgcct cgtcgacttc aagctcgaat
     1681 tcggccgtct ttacgaaggc gatttcagcc gcatcatcct cgccgacgag atcagccccg
     1741 acggctgccg cctgtgggac atgacgacga acgaaaagct cgacaaggac cgcttccgcc
     1801 gcgacctcgg cggcgaggtc gaggcctatc aggaagtcgc gcgccgattg ggcctgatgc
     1861 ccgagggcgg cgagaatgcc gtgctcgacc tcgaaagcca ccgcaagaaa aagggtagct
     1921 gagtttgctc caacccctcc ccttcagggg aggggcagcg aggcttgcga acttgttcgc
     1981 tagccgcagc ggggtgggcg cttcgacctc gcgttgcgcc gcgaaccccc accccaaccc
     2041 ctcccctgaa ggggaggggc tcaatatcct caaatattga tgctcgtcgg aatcaagctc
     2101 ggctgcagat acgggtaagg ctgcatccgc tccgcattgc gctgcacgat ctccgcctcg
     2161 acgcccttga gcgccgcctg aaaccacacc agcgggccct cggccgcggt cacacgcggg
     2221 tcctgaaacc atttgggata gggaaaggcg ttgctgcgat aatcccccag cggccggtaa
     2281 tgcaccgacc ccagcagctc gagtacattg agctgctcga gcgcgagcgc catcggcggc
     2341 atcatcgcga gccagcccgc cttgtcgtgc ccgcgctggc cgttcggcgc cgcctgccag
     2401 cccgcgccgg tgaccgcggg ggcaaaggcc atgatatcct tttgcgggaa attgaccgcg
     2461 gcatgctgcg cgctcgcggt gaacatcacc atcgcgcaca cgtcggcaag ctgtttgcgc
     2521 gtcgtcacgg ggccgaagcc cttgatcttc gcttcgccgg caaggcacgc cgcccaagcg
     2581 ctaagttcgg tgtcggcgac gaggtcggcg tcgtgcgcat aataaagatc gacatattgc
     2641 cgcgcccatt cgtggatcgc gtcccagacg agcagcgcgt cgtcgcggta cggatagtcg
     2701 gcgagcgccg aatcgacccc gacgccgcgc gcggcgaaat cggcgtgcgg catcttgcca
     2761 tggaaatcga acgcgagccg cgcgtcgacc gccgccgcct ggctcgatgc gatcgtgccc
     2821 gcgaaaatat ggtcgatcgg gccgttcgcc gcgatcagcg aggtcgccgc cgcttcgttg
     2881 atgaacagcg tgccctcgaa atggggcacg agcagcgccc atatcgggtg cgcttcggcg
     2941 agatggcggt gcgtcgcgac cgcgaacgct tcgatcacca gatgggtgcg cgccagatgc
     3001 gtgaacagct cgtgataatt gccgtcggcg acctcgacga cgagcttcgc catttcccag
     3061 ccccatagct tgtcggcggc gggcgagggg gtgaagatcg gatagtcggc ggggtcctgc
     3121 ccgcactgga tcgcgaccgg cacgagcgac gatccgcccg gcggcaccgc gaacagcgcc
     3181 atcggctgcc acgcatattt ggcgaggctg ccataggtgc cgggatcgag gacttcgagc
     3241 ggctggtaat cgagcatgaa aaggcggcca tcggcaagcg ccgcctccag cgtatcacca
     3301 ttcaccaccg cggcatattg cgcggcgcgg agcgggaaat gggagggcag cgcagtaccg
     3361 accgcagcga tcagcatgca gttcggcccc tgcacgcgca gccgcgcaaa ctcggcatcg
     3421 tcctggaaca tatagctgac gccggggacg ggcagcgtcg cgaacagcgc gcgataggct
     3481 tcgatgctgc gcaccgtctt gctgccgaga ccgagcgcct cctcgacctt catcagatcc
     3541 tcgagccggg ccttatagcc cttgagcagc gcgacatgcg cgtcgcgctc ggcggcggcg
     3601 acggcttgtt cgacgtcgcc gatcatccgt tcgaagatgt tgccgccggt gtggacgcca
     3661 tgctccgccg cgatcttcgc ggccgacgcc tcgatcgcgt cgcattcggc gagcgcggct
     3721 tcgtaatcgg cgcgcgggct gtcgagctcg cccttgtcga cgccgccaag cttcaccgtc
     3781 agcgcgttgc gcacgatgcc cagcccgacg ccgatcagaa ccacgaacca ggcgatcgtc
     3841 ggctcgtcat tcttgggcac gttcgtcgcc agcggcacgc cgggcagcgt cgggacgtcg
     3901 gtcgtccatg catagacggt ccgcgccgcg gcgag
//
LOCUS       DATMSU010000385         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_177239,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000385 DATMSU010000000
VERSION     DATMSU010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177239"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VN034_10340"
     CDS             complement(<1..328)
                     /locus_tag="VN034_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HWV61044.1"
                     /translation="MANTTLNDRALIRLSGDDVRGFLQGLVTNDTSGNLPVWAALLTA
                     QGKALFDFLIWADEEDVLIDCERDAAEALVKRLTLYRLRRPITIAIEPGLAVHWAPAG
                     DLGAPDP"
     gene            472..1494
                     /gene="pyrC"
                     /locus_tag="VN034_10345"
     CDS             472..1494
                     /gene="pyrC"
                     /locus_tag="VN034_10345"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWV61045.1"
                     /translation="MTDRLTIRRPDDWHVHLRDGAMLAHVAQYTARQFARAIVMPNLS
                     PPVTTAEEGRAYRDRINAAVPAGLDFTPLIVAYLTDHSDADEMARGHAEGVFTAAKLY
                     PAHATTGSAHGVTDVANIMGVLERMQDIGMPLLIHGEVTDHDVDIFDREAVFIERTLE
                     PLVRALPALKIVFEHITTAEAAQFVAGAPANVAATVTPQHLHINRNAMLVGGIRPHAY
                     CLPVAKREHHRLAVRAAATSGSPKFFLGTDSAPHAVHTKEASCGCSGIFNAPYALESY
                     IKAFDEDGALAHFEGFASEHGPRFYGLPLNEGTVTLERGETLVPDRIGEVVPFHAGET
                     LGWRLV"
     gene            complement(1594..2511)
                     /gene="rarD"
                     /locus_tag="VN034_10350"
     CDS             complement(1594..2511)
                     /gene="rarD"
                     /locus_tag="VN034_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HWV61046.1"
                     /translation="MAQTPAPAGSNQGGLPYALAAYGIWGFVPLFFKLIASVPPVEVL
                     AQRIIWSLPLCFVIMLFRRQIGEYLAVLKDWRTLRLLLASSLLIAVNWLVYIYSIFTD
                     HVLAASLGYYLNPLVNVMLGMIFLGERLSKLQLLAVVIAGVGVAILLAGALDTLWISL
                     TLAFSFGIYGLIRKVVPVGSLPGLSVETTVLLLPSLAVSAWYLWAGDGRGFGSDGSVS
                     LLLMAGGVVTAVPLLLFATAARRMSYAALGFVQFLAPTLQFLLSLFVFHEPLKPAQFA
                     CFILIWASIAVFSFDMLRKMRAERMIEVA"
     gene            complement(2532..2753)
                     /locus_tag="VN034_10355"
     CDS             complement(2532..2753)
                     /locus_tag="VN034_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61047.1"
                     /translation="MLNIGSLIIGAIALVLAVFAFIPLLGWANWVIIPFAVIGLALGA
                     MSDKTSGRNLNIVVIVIGVIRLMLGGGII"
     gene            2934..>3269
                     /locus_tag="VN034_10360"
     CDS             2934..>3269
                     /locus_tag="VN034_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61048.1"
                     /translation="MRIFTKGLMGAFVAASVLGTTPLHAGVTTPAKAPGYHEFEQSTN
                     FHRKRKDRRHYSRDERVGRDTRIWRDNDGRYRCKKDNGTTGLLIGGAVGGLAGHEIAG
                     GGDKLLGTVL"
BASE COUNT          644 a         1150 c         1007 g          468 t
ORIGIN      
        1 gcggatcggg cgcacccagg tcgcccgccg gcgcccaatg caccgcgaga ccgggctcga
       61 tcgcgatcgt gatcgggcgg cgcagacgat agagggtcag gcgcttgacc agcgcctcgg
      121 ccgcatcgcg ctcgcaatcg atcagcacat cctcctcatc cgcccagatc aggaaatcga
      181 acaacgcctt gccctgcgcg gtgagcagcg ccgcccacac cggcagattg cccgatgtgt
      241 cgttggtgac gagaccttgc aggaaaccgc ggacgtcatc ccccgacaga cgaatgaggg
      301 cgcggtcgtt gagggtggta ttagccatgg atcggacata gggctgcgcc ccgacgactt
      361 aaagcccctc ccgcttgcgg gaggggttgg ggtgggcagc aacatccgct aagcgcgcga
      421 tggactcgcc cacccccgac ccctcccgca agcgggaggg gggagataaa gatgaccgac
      481 cgcctgacga tccgccgccc cgacgactgg cacgtccacc tgcgcgacgg cgcgatgctc
      541 gcccatgtcg cgcaatatac cgcgcgccag ttcgcgcgtg cgatcgtcat gcccaacctc
      601 tcgccgccgg tgaccaccgc cgaggaaggc cgcgcatacc gcgaccgcat caacgccgcg
      661 gtgcccgccg ggctcgattt caccccgctg atcgtcgcct atctgaccga ccacagcgac
      721 gccgacgaga tggcgcgcgg acacgccgag ggcgtcttta ccgccgccaa actctatccc
      781 gcgcatgcga ccaccggcag cgcgcacggc gtcaccgacg tcgcgaacat catgggtgtg
      841 ctcgagagga tgcaggacat cggcatgccg ctcctcatcc acggcgaggt caccgaccat
      901 gacgtcgaca ttttcgaccg cgaggcggtg ttcatcgaac gcacgctcga accgctcgtc
      961 cgcgccctcc ccgcgctcaa gatcgtcttc gaacatatca cgactgcaga ggccgcgcag
     1021 ttcgtcgcgg gcgcccccgc caatgtcgcc gcgacggtca cgccgcagca tctccatatc
     1081 aaccgcaacg cgatgctcgt cggcggcatc cgcccgcacg cctattgcct gcccgtcgcc
     1141 aagcgcgagc atcaccggct cgcggtgcgc gccgccgcga cttcgggctc gcccaaattc
     1201 ttcctcggca ccgacagcgc gccgcacgcg gtgcacacga aggaggcatc ctgcggctgc
     1261 tcgggcatct tcaatgcccc ctatgcgctc gaatcctata tcaaggcctt cgacgaagac
     1321 ggcgcgctcg cccatttcga aggcttcgcc agcgaacacg gcccgcgctt ctacggcctg
     1381 ccgctcaacg agggcaccgt cacattggaa cgcggcgaga cgcttgtccc cgaccgcatc
     1441 ggcgaagtcg tccccttcca cgccggcgaa acgctgggct ggcggctggt ctgacttcct
     1501 tcgtcacccc ggacttgatc cggggtccat gcagcggccg cgaccatgga tgccggatca
     1561 agtccggcat gacgaggatg gaagaggcgg cccctacgcc acctcgatca tccgctcggc
     1621 gcgcatcttg cgcagcatgt cgaaactgaa caccgcgatg ctcgcccaga tcaggatgaa
     1681 gcaggcgaac tgcgcgggct tcagcggctc gtgaaagacg aacaggctca agaggaactg
     1741 gagcgtcggc gcgaggaact ggacgaagcc gagcgcggcg tagctcatcc gccgcgccgc
     1801 ggtcgcgaag agcagcagcg gcaccgcggt gacgacaccc cccgccatca gcagcaggct
     1861 gaccgatccg tccgatccga agccgcgccc gtcgcccgcc cacagatacc aggccgacac
     1921 ggcaagcgac ggcaacagca gcaccgtcgt ctcgaccgaa agcccgggca gcgaccccac
     1981 cggcacgacc ttgcggatca ggccatagat gccgaacgag aaagcgagcg tcaggctgat
     2041 ccataaagtg tcgagcgcgc ccgcgagcag gatcgcgacg ccgacgcccg cgatcacgac
     2101 cgccagcagc tgcagcttcg acaatcgctc gccgaggaag atcatgccga gcatcacgtt
     2161 gacgagcggg ttgagataat agccgaggct cgccgcgagc acatgatcgg tgaagatcga
     2221 atagatatag acgagccagt tgaccgcgat cagcaacgaa ctcgcgagca gcagccgcag
     2281 cgtccgccaa tccttgagca ccgccagata ttcgccgatc tgccgccgga agagcatgat
     2341 cacgaaacag agcggcagcg accagatgat ccgctgcgcc agtacctcga ccggcggcac
     2401 gctcgcgatc agcttgaaga agagcgggac gaagccccag atgccatagg ccgccagcgc
     2461 atagggcagc ccgccctgat tgctccccgc gggggccggt gtctgtgcca tggcgctcga
     2521 cttctaaagg atcagatgat gccgccgccg agcatcaggc ggatgacgcc gatgacgatg
     2581 acgacgatat tgaggttccg cccgctcgtc ttgtccgaca tcgcgccgag cgcgagcccg
     2641 atcacggcga aggggatgat cacccagttc gcccagccga gcagcggaat gaacgcaaaa
     2701 acggccagaa ccaaggcgat ggcgccgatg atgagcgatc cgatattgag catggacagg
     2761 gttttcgcac gccgtccgcg atgcggcaag agcctttcgg ttcgtcgatg gatgcgctcg
     2821 tttcatcttg ttgccttgcg ttcatgcaac gcgatcggga gccggctcgt tttccccctc
     2881 gaagcgcccg gacctgccca gggacggagc gcggaaccag gaaaggactt gttatgcgta
     2941 tcttcaccaa aggcctgatg ggcgcgttcg tcgccgccag cgtcctcggc accacgccgc
     3001 tccacgccgg cgtgacgacc cccgccaagg ccccgggcta tcatgaattc gaacagtcga
     3061 cgaacttcca ccgcaagcgc aaggatcgcc gccattattc gcgtgacgag cgcgtcggcc
     3121 gcgacacgcg catctggcgc gacaacgacg gccgctatcg ctgcaagaag gataacggca
     3181 cgaccggcct tctgatcggc ggcgccgtcg gcggcctcgc cggccatgaa atcgccggcg
     3241 gcggcgacaa gctgctcggc accgtgctc
//
LOCUS       DATMSU010000386         5394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_167452,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000386 DATMSU010000000
VERSION     DATMSU010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5394
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167452"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..794)
                     /locus_tag="VN034_10365"
     CDS             complement(9..794)
                     /locus_tag="VN034_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61049.1"
                     /translation="MPGLLENKVVIISGVGPGMGQAMARIAAAEGAKVGLGARNRDFI
                     DQVADEIRASGGEAVAVPTDVGDADQCAALAKATADTFGRIDGLVNTAYIHGEWVTVD
                     EADPEDWARVFDVNCLGALRMTQAVLPAMKAQRGGAVVNVATMSQVNPFPGEGGYATG
                     KGGLTTMTRHMAKDLGRFNIRVNATRMGWIGGKPVYDYIDRATAGGQDRDTVIGEIIA
                     RIPLGVIPPEEDCAKSVLFFLSDYAKMVTGATLDVNGGQYMAP"
     gene            complement(778..2031)
                     /locus_tag="VN034_10370"
     CDS             complement(778..2031)
                     /locus_tag="VN034_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HWV61050.1"
                     /translation="MRGDPFSPVMIGPVTLRNRFIRSGANEMMSKATFPTRALLDFHR
                     AYARGGVGMTTLAYLAVSPDGRTLPGQGMLSRDSVPHYRAVTDAIHAEGAKASAQITH
                     GGSFCQIDDLSTPRAMSASAGIDKMGVLKGRFLQRAMTHADMAMVTGEFARAAELAEE
                     AGFDALEIHMGHGYLINQFISPLSNFRKDDYGGSAANRARFPAEVLAAVKQATDGRLA
                     ILAKINLTDAVPGGATVDDAIVTARALEAAGADMLVLSAGRNIESTWAMFGSALPYDD
                     LAAMQGGLLAKLQFAILKRSVPKLPPFRENYLMEPALKLKAALGPTPMKLAYLGGIQS
                     LTGARRAAAEGFDAIALARALIHDPALIAKWQSGATDVSGCTACNRCVAAMYGPSGTY
                     CPEVGNALDPGLRDIFAGEKYAGPA"
     gene            complement(2053..2847)
                     /locus_tag="VN034_10375"
     CDS             complement(2053..2847)
                     /locus_tag="VN034_10375"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWV61051.1"
                     /translation="MARLAGKVAVVTGAAQGMGAATARLFAAEGAKVVLCDVIEDKGQ
                     ETASEIGESAVFQKLDVRSEADWERVVAETVQRFGKLDILINNAAVVHFSPIEAMPAE
                     DIERVLAINVMGTMLGAKYAARAMTEAGRGVIVNISSVDGLRGCNGLSAYTASKWAVR
                     GLSKSLAYELGPRGIRVCTVHPGGVNTQMGNPTGLTGDAVNVGYERVPLQRIGEPEEI
                     ARASLFIASDDASYISGAELAVDGGWSAGYYQPFLPGAPASLMGQP"
     gene            complement(2883..3962)
                     /locus_tag="VN034_10380"
     CDS             complement(2883..3962)
                     /locus_tag="VN034_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWV61052.1"
                     /translation="MATTADYGLGEQVFPRGWFMIAAAAELEGTKPLAVRFFGRDRVL
                     YRGESGQVYLVDAYCPHMGAHLAKNETSFVIRDNEHVQGDSIRCPYHGWRFGPDGKCN
                     QIPYSPQRIPAAAKLENWPVIERAGCIWMWHDPEGLEPEYELPPFAEYDQPHWVKWEL
                     DRLGELASHPVEILDNMADRAHFEPVHGNDAVDVFENDFDGHRVVQRLRAGHRTLQGD
                     APLETDTWYTGPGILQSRMSGQFPSIIMIAHTPVDDGVVKVWHALLVKVPNAEPTADD
                     VAMARAYQAAACAAFAQDFEIWSNKKPCVNPMAVQGDGPIGKLRIWYRQFYNPRARAE
                     EFQKRVNGTHVTRGTLSAPFEQKVA"
     gene            4103..4879
                     /locus_tag="VN034_10385"
     CDS             4103..4879
                     /locus_tag="VN034_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61053.1"
                     /translation="MGEVRQFPEGAALIFGATGGIGAAVAGEFADAGSDVAIAWRSKQ
                     AEAEALAERIEASGRKASLHQCDVTDDAGLAAAIADAVAAHGRIHTMLWGAGPLVAQV
                     HLADTTPEQWRRAIDVEVHGFFNAAHHVIPHMRAHGGGSFVHLGSAGDLRWPDKDGLS
                     VAPKAANEALVKGIAREEGKHGIRANSILVGVIEAGMFLELSKQGVFDDAWTAEVHKN
                     LSLKRWGRPEEIGHAAVFLASSKAAYITGQQIAVAGGYGG"
     gene            complement(4909..>5394)
                     /locus_tag="VN034_10390"
     CDS             complement(4909..>5394)
                     /locus_tag="VN034_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWV61054.1"
                     /translation="DSAGGNLSAALCLDARDNGGPAIAHQLLLYPVIDNDFGRASHSE
                     NASGYLLSTAMIQWFWAQYLGDAGRTPEALAAPIRAASLAGLPSATVITAEYDPLRDE
                     GNAFAARLAAEGVAVTHSEVPGVIHGFASMLGMLPQADIAVGTAARGLRTAFKMDAVT
                     A"
BASE COUNT          878 a         1793 c         1767 g          956 t
ORIGIN      
        1 cgtcatcgtc atggcgccat atactggccg ccgttgacgt cgagcgtcgc gccggtgacc
       61 atttttgcat aatcggagag gaagaagagc acgctcttcg cgcaatcctc ctcgggcgga
      121 atgacgccga gcgggatgcg cgcgatgatc tcgccgatca ccgtatcgcg gtcctgtccg
      181 cccgcggtgg cgcggtcgat atagtcataa acgggcttgc cgccgatcca gcccatccgc
      241 gtcgcgttga cccggatatt aaagcggcca agatccttcg ccatatgtct ggtcatggtg
      301 gtgagcccgc ccttgccggt ggcatagccg ccctcgccgg gaaagggatt gacctgcgac
      361 atggtagcga cattgaccac cgcgccgccg cgctgcgcct tcatcgcggg caataccgcc
      421 tgcgtcatcc tgagcgcgcc gaggcaattg acgtcgaata cccgcgccca atcctcggga
      481 tcggcctcgt ccaccgtcac ccattcgccg tggatatagg cggtgttaac cagcccatcg
      541 atccgtccga acgtgtcggc ggtcgctttc gccagcgcgg cgcactggtc cgcatcgccg
      601 acgtcggtcg gcaccgcgac cgcctcgccg ccgctcgcgc ggatctcgtc ggcaacctga
      661 tcgatgaaat ccctgttgcg cgcgccgagc ccgaccttgg cgccctcggc ggcggcaatc
      721 cgcgccatcg cctgccccat gccgggaccg actccgctga tgatgacgac cttgttctca
      781 agcaggcccg gcatattttt ctcccgcaaa aatgtccctg aggccggggt cgagcgcgtt
      841 gccgacctcc gggcaatatg ttcccgacgg gccgtacatc gcggcgacgc agcggttgca
      901 ggcggtgcag cccgaaacat cggtcgcccc gctttgccat ttcgcgatca atgccggatc
      961 gtggatcagc gcgcgtgcca gcgcgatcgc atcgaagcct tcggccgccg cccgccgcgc
     1021 cccggtgagc gactggatgc cccccagata ggcgagcttc atcggcgtcg ggcccagcgc
     1081 cgccttcagc ttcagagcgg gctccatcag ataattttcg cggaacggcg gcagcttcgg
     1141 cacgctgcgc ttcaggatcg cgaattggag tttcgccagc agcccgccct gcatcgcggc
     1201 aagatcgtca taggggagcg cgctgccgaa catcgcccag gtgctttcga tgttgcgccc
     1261 cgccgacagc accagcatgt cggcgcccgc cgcttcgagc gcgcgcgcgg tcacaatcgc
     1321 atcgtcgacc gtcgcaccgc ccggcaccgc atcggtcagg ttgatcttgg cgaggatcgc
     1381 gagccgcccg tccgtcgcct gtttcaccgc tgccagcact tcggcgggaa agcgcgcgcg
     1441 gttcgctgcc gacccgccat agtcgtcttt gcggaagttg gagagcggcg agatgaactg
     1501 gtttatcaga tagccgtgcc ccatatggat ttcgagcgca tcgaaacccg cttcttcggc
     1561 cagctcggcg gcgcgcgcga actcgcccgt caccatcgcc atgtcggcgt gggtcatcgc
     1621 gcgctgaagg aagcggccct tgagcacccc catcttgtcg atcccggccg atgccgacat
     1681 cgcgcgcggg gtcgacaggt cgtcgatctg gcaaaagctg ccgccgtggg tgatctgcgc
     1741 cgacgccttg gcgccctcgg cgtggatcgc atcggtgacc gcgcggtaat gcggcacgct
     1801 gtcgcggctc agcatcccct gtccgggcag cgtgcggccg tcgggcgaaa ccgcgagata
     1861 ggcgagcgtc gtcatcccga cgccgccgcg cgcataggcg cggtgaaagt cgagaagcgc
     1921 gcgggtgggg aaggtcgcct tgctcatcat ctcgttcgcg cccgaacgga tgaagcgatt
     1981 gcggagcgtg actggcccga tcatcaccgg gctgaacgga tcgccgcgca tgggttcagg
     2041 cgcccggggg cgtcacggct gacccatcag gctggccggc gcgccgggca ggaagggctg
     2101 gtaatagccc gccgaccagc cgccatcgac cgccagctcg gcgccgctga tatagctcgc
     2161 atcgtcggag gcgatgaaca ggctggcgcg cgcgatttcc tcgggctcgc cgatgcgctg
     2221 gagcgggacg cgctcgtaac cgacattgac ggcgtcgccg gtgaggccgg tcgggttgcc
     2281 catctgcgtg ttcacgccgc cggggtggac ggtgcagacg cggatgccgc gcggccccaa
     2341 ttcataggcg agcgatttcg acagcccgcg caccgcccat ttgctggcgg tataggccga
     2401 caggccgttg cagccgcgca ggccgtcgac cgacgaaatg ttgacgatca cgccgcggcc
     2461 ggcctcggtc atcgcgcgcg cggcatattt ggcgccgagc atcgtcccca tcacattgat
     2521 cgcaaggacg cgttcgatat cctcggcggg catcgcctcg atcgggctga aatgcacgac
     2581 agcggcattg ttgatcagga tgtcgagctt gccgaagcgc tggacagtct cggcgaccac
     2641 gcgttcccag tcggcctccg atcgcacgtc gagcttctgg aaaacggcac tctcgccgat
     2701 ttcgctcgcg gtttcctgtc ctttgtcctc gatcacgtcg cacagcacca ccttggcgcc
     2761 ctcggccgcg aaaagccggg cggttgcggc gcccatgccc tgtgccgcac ccgttacgac
     2821 cgcaacttta cccgcgagtc ttgccacgca ttttctcctg atttctgtct gtgttgccgc
     2881 cctcaggcga ccttctgttc gaacggcgcg gacaaggtgc cgcgggtgac gtgggtgccg
     2941 ttgacgcgct tctggaattc ctcggcgcgc gcgcgggggt tgtagaactg gcgataccag
     3001 atgcgcagct tgccgatcgg gccgtcgccc tgcaccgcca tcgggttgac gcacggtttc
     3061 ttgttcgacc agatttcgaa atcctgcgcg aaggcggcgc aggccgcggc ctgataggcg
     3121 cgcgccatcg cgacatcgtc cgccgtcggc tcggcattgg gaaccttgac cagcagcgcg
     3181 tgccacacct tgaccacccc gtcgtcaacg ggggtgtgcg cgatcatgat gatcgacggg
     3241 aattgccccg acatgcgcga ttgcaggatg cccggtccgg tataccaggt gtcggtttcg
     3301 agcggcgcgt cgccctgaag cgtgcggtgc cccgcgcgca gccgctggac gacgcggtgg
     3361 ccgtcgaaat cattctcgaa cacatcgacg gcatcgttgc cgtggacggg ctcgaaatgc
     3421 gcgcggtcgg ccatattgtc gaggatctcg accggatggc tcgcgagttc gcccagccgg
     3481 tcgagctccc acttcaccca atgcggctga tcatattccg cgaacggcgg cagctcatat
     3541 tcgggttcga gcccttcggg atcgtgccac atccagatgc agccggcgcg ttcgatcacc
     3601 ggccaattct cgagcttggc ggccgcgggg attcgctgcg gcgaataggg gatctggttg
     3661 cacttgccgt cgggaccgaa gcgccagcca tgataggggc agcggatcga gtcgccctgc
     3721 acatgctcat tgtcgcggat cacgaagctc gtctcgttct tcgccagatg cgcgcccatg
     3781 tgcgggcaat aggcatcgac cagatagacc tgccccgact cgccgcgata gagcacccgg
     3841 tcgcggccga aaaagcggac ggcgagcggt ttcgtccctt cgagttcggc ggcggcggcg
     3901 atcatgaacc agccgcgcgg aaagacctgt tcgcccaggc catagtctgc ggttgtcgcc
     3961 attgaaaatc tcccgtcatg cccggtttcg gagcggacat tgtcactatg cgttttgaaa
     4021 acttgctata ccatcgcatt tcgataacat atctcacaat taacaacatt atgcaatatt
     4081 ctgcgcgtca aggagggtga atatgggtga agttcgtcaa tttcccgagg gcgcggcact
     4141 gattttcggt gcgaccggcg gcatcggcgc cgcggtcgcc ggcgaattcg ccgacgcggg
     4201 aagcgatgtc gcgatcgcgt ggcgttcgaa acaggcggaa gccgaagcgc tggccgaacg
     4261 catcgaggca tcgggccgca aggcgagcct gcaccaatgc gacgttaccg atgatgccgg
     4321 gctcgccgcc gcgatcgccg atgccgtcgc cgcgcacggg cgcatccaca ctatgctgtg
     4381 gggcgcgggc ccgcttgtcg cgcaggtgca cctcgccgac acgacacccg aacaatggcg
     4441 ccgcgcgatc gatgtcgaag tccacggttt tttcaacgcg gcgcaccatg tcattccgca
     4501 tatgcgcgcg cacggcggcg gctcgttcgt gcatctgggg tcggcgggcg acctgcgctg
     4561 gcccgacaag gacgggctgt cggtcgcgcc caaggccgcg aacgaggcgc tggtcaaagg
     4621 tatcgcgcgc gaggagggga agcacggtat ccgcgccaac tccatcctcg tcggcgtgat
     4681 cgaggccggc atgttcctcg agctttcgaa acagggcgtg ttcgacgacg cgtggaccgc
     4741 cgaggtgcac aagaatctgt cgctcaagcg ttgggggcgt cccgaggaaa tcggccatgc
     4801 cgctgtcttc cttgcctcgt ccaaagctgc ctacataacc gggcagcaga ttgcggtggc
     4861 gggcggttac gggggctgaa cccgacggtg cagacccccg ccgcgcgatt acgccgtgac
     4921 ggcgtccatc ttgaaagcgg tccgaaggcc ccgggccgcg gtgcccaccg cgatgtcggc
     4981 ttgcggcagc atgccgagca tgctcgcaaa gccgtggatc acgcccggca cctcgctgtg
     5041 cgttaccgcg acaccttcgg ccgcaagccg cgcggcaaag gcattgcctt cgtcgcgcag
     5101 cggatcatat tcggcggtga tcacggtcgc cgacggcagg ccggcgaggc tggcggcgcg
     5161 gatcggcgcc gccagtgctt cgggcgtccg tcccgcgtcg cccagatatt gcgcccagaa
     5221 ccattggatc atcgcggtgc tgaggaggta gcccgaggcg ttttcgctgt gcgacgcgcg
     5281 gccgaaatca ttgtcgatga cggggtagag gagcagctgg tgcgcgatcg cggggccgcc
     5341 attgtcgcgc gcgtcgaggc agagcgcggc ggacagattg ccgccggcgc tgtc
//
LOCUS       DATMSU010000387         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_177315,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000387 DATMSU010000000
VERSION     DATMSU010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177315"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1435)
                     /gene="mobQ"
                     /locus_tag="VN034_10395"
                     /pseudo
     CDS             complement(8..1435)
                     /gene="mobQ"
                     /locus_tag="VN034_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
     gene            1609..1917
                     /locus_tag="VN034_10400"
     CDS             1609..1917
                     /locus_tag="VN034_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HWV61055.1"
                     /translation="MRKVRDYDAELKALGDKARAIKAKKVQQLGELVTSTGADALDAD
                     VLAGALLHIVAEAQVEGNREAWRSDGAAFFQGRGRKAGRRSDGNAEGGKETDAGEAQG
                     "
     gene            1955..2179
                     /locus_tag="VN034_10405"
     CDS             1955..2179
                     /locus_tag="VN034_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HWV61056.1"
                     /translation="MARRERTRHLIELGGLVQKAGLVDLTDDDRATLYGAMLELAAKA
                     REENAGDVLALWKRRGKRAFDAEAEGNEAP"
     gene            2176..2466
                     /locus_tag="VN034_10410"
     CDS             2176..2466
                     /locus_tag="VN034_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61057.1"
                     /translation="MTTLDVEAIRCQVRALDFTRGTPAEVALWREDDADARANLAIED
                     MALDAQEEMLFDMLREEAVPPPLATAIVLKLLGHPDADPALAISPATIMAAR"
     gene            2473..>3258
                     /locus_tag="VN034_10415"
     CDS             2473..>3258
                     /locus_tag="VN034_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013038680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HWV61058.1"
                     /translation="MEWFRQLGRALRNLARISRQNPIWAITALTLSPIALIRHLFGVA
                     MLFLIVGLVLGLGMPLILGKLLGLPHDSNIYQIVMMLTGLVVILVALRALFQPLILKY
                     GGPDSDDTHGSARFATARETKPLANADSGLLIGRDMKTRKLLRYPGPAHLLTIAPTRT
                     GKGVGTIIPNLINYPGPVVCIDPKGENAIVTARQRARFGPVHVLDPFGITGQPSAAFN
                     PLDRIDPAGLDLADDAMTLADALVYDAPGEAGEAHWNDEAKALI"
BASE COUNT          531 a         1193 c          986 g          548 t
ORIGIN      
        1 cggcctagat cgcctgcaac tgctcggagt cgccgacaag caccaccttc gcccctgccc
       61 gctcagcctc ggacagcacc cgctccatct ggcgcgtgcc gatcatgccg gcctcgtcga
      121 tcaccagcac gtcgcgcggc cccagcagct cgcggccctg atcccactga tattccatgc
      181 tggcgatcgt gcgcgaggcg atgccggaac cgccctcaag cccctcggcc gcgatccccg
      241 acaaggccgc gccgcgcacc tgatagccgg cgcgctccca ttcgtcgcgc gcgactccca
      301 gcatggccga tttgccggtg ccggcgtagc cgacgacgat tgccaggtct tccttgccgg
      361 tgatatgcgc cagcgcgtcc ttctgctggc ccgccagcac caggctacca ctcccggcga
      421 cgtcccgctc cctttgcagc gacgtcgccg ggagaccatg cccctcccgt cctgcaaggc
      481 gatcgccggc gcgctccaac cgctgctcgg tgtcgatcat gtcgcgcgag gtaaagcggt
      541 cctcgccctt cccgtcctgc cccagcgcca ccagctcggg cgaggtccgc accgcgctca
      601 tcacctggtc gaactgatcc ttgccgtcgc tgtgccggaa cgcgaactgc gccaggtcgc
      661 ggcgcgtgaa cgtcgcctgc tgccgggtga tcgcgtcgag cgcgatttcg ggccgcgcaa
      721 tgatcttctc gccattctcc cgcgcgatcc gggcatggtc ctcgacccgc tcggcctcaa
      781 gtccctgttc gggcatacgc gacgccgccg gcccgatctt gtgctgcggc tcaagctcga
      841 tcccctgcgc ctgcaacgtc cggtgatcta tgcgagcgtc aatatccagc tcggccaggc
      901 gctcgttgac atgatcggcc catgcctcgc gccagccctt caacagctcg gtgctgttcc
      961 actcgcgcac cttcgccccg aagccttccg gccctacctc gcgcatcgac agcatgacat
     1021 gcgcgtgtgg cttggggctt ccgtccttcc ccatgtccca atgcacattg aggtccgcca
     1081 ccatgccgct ctcgacgaac tgcttttcga cgaaatcgcg cgcgaggcta acgccctgct
     1141 gctggttcat ctcgcgcgga atagcgaact cgacttcgcg cgcgagctgc gcatccttgc
     1201 gcttctcgcc ggcctcgacc tcgttccaca aggtcgcgcg atcgtttaaa cgctccggcg
     1261 cgccctcggg cagcatgatt tcagaatgga cgacgccggc cttgttcgag aaatcatggt
     1321 cgcgcccgag tcggtcatcg tgcagccgtt cggcggcacg ataagctgcg ctcgcaacgg
     1381 cgctcgatcc gttggcgcgg ctgatgacct tggccgagaa atggtagatc gccataacgg
     1441 ccgctatact acctttctta gcgcacgtcg gcaacgacgt ataagcgcgc actcacactc
     1501 tctgccgttc gcgtgattga cttcgccgca tttcttgctt cgggcagttc ctcgcgcgcg
     1561 cgatcgtgct acgctgcggc tcccgatcat gggcgcgagg gagagaatat gcgcaaggtc
     1621 cgcgactatg acgccgagtt gaaggcgctg ggcgacaagg ccagggcgat caaggcgaaa
     1681 aaggtccagc aattgggcga gctggtcacg tccaccgggg ccgatgcgct cgatgctgat
     1741 gtgctggccg gcgcgctgct ccacatcgtc gccgaagcgc aggttgaggg aaaccgggag
     1801 gcgtggcgct ccgatggcgc ggccttcttt caagggcgcg ggcgcaaggc tgggcgacgc
     1861 tctgacggca acgcggaagg cggaaaggaa actgacgcag gcgaggcgca gggttgaggc
     1921 cgccgcgcgc cgcaccgata cgagaggatg ggtcgtggcc cgacgcgaac gcacccgcca
     1981 cctgatcgag ctgggcggcc tcgtccagaa ggccggcctc gtcgatctta ccgacgacga
     2041 ccgcgccacc ctctacggcg cgatgctcga actggcggcg aaggcgcgcg aggaaaatgc
     2101 cggcgatgtg ctggcgctct ggaagcggcg cggcaaacgc gcgttcgacg cggaagcgga
     2161 agggaacgaa gcaccatgac gacgctcgat gtcgaggcca tccgctgcca ggtccgcgcg
     2221 ctcgatttta cgcgcggcac cccggccgaa gtcgccctat ggcgcgagga cgatgccgac
     2281 gcgcgcgcca atctcgcgat cgaagacatg gcgctcgacg cgcaagagga aatgctgttc
     2341 gacatgctgc gcgaggaagc cgtgccccct cccctcgcca ccgcgatcgt cctcaaactg
     2401 ctcggccatc ccgatgccga cccggcgctg gcgatctcgc cggcgacgat catggcggcc
     2461 aggtgatcgg ctatggaatg gttccgccag ctcggccgcg cgctccgcaa cctcgcccgc
     2521 atatcgcgcc agaatccgat atgggcgatc acggccctga ccctcagccc gatcgcactc
     2581 atccgccacc tgttcggcgt cgccatgctg ttcctgatcg tcggcctggt cctcgggctg
     2641 gggatgccgc tcatcctcgg caagctgctc ggcctgcccc acgattccaa tatctaccaa
     2701 atcgtgatga tgctgacggg cctagtcgtc atcctcgtgg cgctgcgcgc cctcttccaa
     2761 ccgctgatcc tgaaatatgg cggccccgac agcgacgaca cccacggctc ggcccgcttc
     2821 gccacagcgc gcgagacaaa gccgctcgcc aacgccgatt ccggcctgct gatcggccgc
     2881 gacatgaaaa cccgcaagct gctgcgctat cctggccccg cccatctgct gacgatcgcg
     2941 cccacccgca ccggcaaggg agtgggcacg atcattccca acctgatcaa ctatcccggc
     3001 ccggtcgtgt gcatcgaccc caagggcgag aacgctatcg tcaccgcgcg ccagcgcgcc
     3061 aggttcggcc cggtccatgt cctcgacccg ttcggcatca ccggccagcc ctcggccgcc
     3121 ttcaatccgc tcgatcgcat cgaccccgcc ggcctcgatc tcgccgacga cgccatgacg
     3181 ctcgccgatg cgctggtcta tgacgccccc ggcgaagcgg gcgaggcgca ttggaacgac
     3241 gaagccaagg cgctgatc
//
LOCUS       DATMSU010000388         6069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_130220,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000388 DATMSU010000000
VERSION     DATMSU010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6069
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130220"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VN034_10420"
     CDS             complement(<1..186)
                     /locus_tag="VN034_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HWV61059.1"
                     /translation="MITMLSLLLAAANPSAAPVATVDAGTLSGARDGDVRIFKGIPYA
                     APPTGEFRWRPPAPAAPW"
     gene            complement(186..2411)
                     /locus_tag="VN034_10425"
     CDS             complement(186..2411)
                     /locus_tag="VN034_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61060.1"
                     /translation="MFNILTRGRLAVLAAGTALTASVPALAQAAAAEATSRADEIVVT
                     AQKREERLQDVPISISVVSPEQLAAFAMNEATDLQYVVPGLTLINASSTRNSGFFVRG
                     IGTSSFSSESIEGSTAFVVDGVVLGQSGASFADLPDIERIEVLRGPQGTLFGKNASAG
                     VVNVTTRDPGDEFEGKVSGSWAWPNDDRRIAAYIGGPIAEGVGFTLSGRINKRDGYVK
                     NLADDRVFNGRDEWGVRGKLKLEPVDGLSLMVTGDYYKRDAACCIWTLRDIPGVPSVS
                     ETLSLIAGIEPGRDNLEQNIGGELFSKNEIYGTALQADYALGDYMLTSITAWRGYRSF
                     DNNDADSSPIDLLDVNYGRFRQRQFTQELRLASPVGGTVDYVVGLFYFDAKVYSRSVQ
                     RFPTLPFPFFNKEVDNRARTRNAAIFGQANIHITDDFRLIAGGRLLHEKAEASKDRFD
                     FALNLTSSAEAKKSDTALVWRGGLQYDFTDDIMAFATVTRGYKGGGYDTNIGLATLPD
                     VEPERPTNIELGLRTAFPGAGLIFNLTAFHTKVAGYQASARDAGPPPVTRILNGEAKT
                     QGIEADFAWRPSADADWSIYGSAALIDGAWGDFANAPCYSGQTAAQGCVGEVQDLTGA
                     RLPWSSRFSGNIATNFTQPLSGDLKLVFDLGLNYRSSQVIAFPNDPRALQKGFALVNA
                     SVALAGGEAWRVSLFGKNLTDKRYSIVDFTTPLGSPGSYSQFIPYEAQRVIGLSAEFG
                     F"
     gene            complement(2506..3852)
                     /locus_tag="VN034_10430"
     CDS             complement(2506..3852)
                     /locus_tag="VN034_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HWV61061.1"
                     /translation="MAASRLSRSRKLAVGVGDFGFNLYWQPASLYLLYFYTDVLKLPP
                     AVAGGIYMVALIWDAVLDPIVGLTADRTRSRFGRYRPYLLFGAPLLAASFVLLFLVPG
                     SPSAWAVLLAGATHFLFRTFYAVVSVPYAALTARVTRDAAVRADITGIRMVSATLAGV
                     LVATLTLPLAELFGGGQSGWTAVACLYAAIATVVLLATAQGVRPLDLPTHSDAVSPPV
                     ADKLRATAANWPLFLVLGAVAVSSFAGTIFQKNLLYYFKYVVGDASLGGAALGFCAIV
                     SAVFVPVFALVARRWGKRRAWLIGIVPSLAGLVLWRLADGQGAAALFVALGVMSMGSA
                     AYYVCFWAMLPDTVEFAEWKTGIRSESFAFGLTMLGQKAALGLGAGLLGVMLAHIGYV
                     ADAVQAPGTLADLKAMMFWIPFAGGILTALLIGFYPITPDRHRQMVSEIAARGTTD"
     gene            3981..4673
                     /locus_tag="VN034_10435"
     CDS             3981..4673
                     /locus_tag="VN034_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61062.1"
                     /translation="MTSTVPASAPPRPGGKSASGRSRAGSSTRQQRLEHTRDAILASA
                     LGHFSRRGFEGASLRDIAQEAGVQHGMIRHIYQTKEELWRQAISFLFERMEGEMPREI
                     SEVHTGEAPERFRTFIHWYVGYCARHPEHARLMIQQSILEGPELAWTAERYIRGRHQW
                     HLPFIEKLQESGHLPQVDPISIVLMVTAACQMPFLLAPEILAATGKNFLTNAAIEAHA
                     DAVTKIFLRKAA"
     gene            complement(4733..5179)
                     /locus_tag="VN034_10440"
     CDS             complement(4733..5179)
                     /locus_tag="VN034_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV61063.1"
                     /translation="MPHQGANLDEIDERLLTLLRDDARQTIAQLAKELGLSRGAIYSR
                     LARLEEDKIVAGYTVRLGNAFAASRVRAHMMIKTLPRFHREVEQALAALPLVQAIHAI
                     SGEYDIIAMLEAEDSAQLNELIDDVGLFEGVERTTTSVILVTKIER"
     gene            complement(5179..6021)
                     /locus_tag="VN034_10445"
     CDS             complement(5179..6021)
                     /locus_tag="VN034_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HWV61064.1"
                     /translation="MLVAFSVAPSGGEGADASVHDAVAAAVRVLEDNPIFNAGRGAVF
                     TAEGRNELDAAIMDGKTQKAGAVAGVTRTRHPIDLARAVMDKSPHVMLARDGADRFSV
                     EQGLEQVEPAWFRTEERWQQLLNWRKKNQAAVDPTHLFGTVGAVALDAEGNLAAATST
                     GGMTGKRWGRIGDSPIIGAGTYAKNGQCAVSATGSGEYFIRESAARQVCDRVAWKGES
                     LKDAAQGAIMAVGAIGGDGGLIAMGPDGRPAFAINDLGMYRGRMSAGEGPQTAIFADE
                     KLAD"
BASE COUNT         1129 a         1995 c         1916 g         1029 t
ORIGIN      
        1 ccacggcgcg gccggcgcgg gcggtcgcca gcgaaactcg cccgtcggcg gcgcggcata
       61 gggaatgccc ttgaagatgc ggacatcacc atcgcgcgcg cccgaaagcg tgccggcatc
      121 gaccgtcgcg accggagccg cactcgggtt tgccgcggcg agcaggagtg aaagcatggt
      181 gatcatcaga aaccgaactc cgctgaaagc ccgatgacgc gttgcgcttc ataggggatg
      241 aactggctgt agctgccggg tgagccgagc ggcgtcgtga aatcgacgat gctgtaccgc
      301 ttgtcggtca gattcttgcc gaacagcgac acgcgccacg cctcgccgcc cgccagagcg
      361 accgaggcgt tgaccagagc aaagcccttt tgcagcgcgc gcggatcgtt cgggaaggcg
      421 atgacctggc tgctgcgata gttgaggccc aggtcgaaaa cgagcttcag gtcgccgctg
      481 agcggctggg tgaagttcgt cgcgatattg ccgctgaacc gcgacgacca ggggagccgg
      541 gcgccggtca gatcctgcac ctcgcccacg cagccctgcg ccgccgtctg gccgctgtag
      601 cagggcgcgt tggcgaaatc cccccacgcg ccatcgatca gcgcggccga gccatagatc
      661 gaccagtcgg cgtcggcgct cgggcgccat gcgaaatcgg cctcgatgcc ttgcgtcttt
      721 gcctcgccgt tcaggatgcg ggtgaccggc ggcggccccg cgtcgcgcgc cgatgcctga
      781 tagcccgcga ccttggtgtg gaaggcggtg aggttgaaga tcagcccggc gcccggaaag
      841 gcggtgcgca ggccaagctc gatattggtc gggcgttcgg gctcgacgtc gggcaaagtg
      901 gcgagtccga tattggtatc gtatccgccg cccttgtagc cgcgcgtcac cgtagcaaag
      961 gccatgatgt cgtcggtgaa atcatattgc agaccgccgc gccatacgag cgcggtatcc
     1021 gattttttcg cctcggcgct gctcgtcaga ttgagcgcga aatcgaaccg gtccttcgac
     1081 gcctccgctt tttcatgcag caggcgccca ccggcgatca gacggaaatc gtcggtgata
     1141 tggatgttcg cctggccgaa gatcgccgcg ttgcgcgtcc gcgcgcgatt atccacctcc
     1201 ttgttgaaga agggaaaagg caaggtcgga aaccgctgga ccgagcgcga atagaccttg
     1261 gcgtcgaaat agaagaggcc gaccacatag tcgaccgtgc cgccgacggg cgaggcgagc
     1321 cgcaattcct gcgtgaactg gcgctggcgg aaccgcccgt aattgacgtc gagcaggtcg
     1381 atcggcgagg aatcggcgtc attattgtcg aaactgcgat agccccgcca cgccgtgatc
     1441 gacgtcagca tataatcgcc cagcgcatag tcggcctgca gcgcggtgcc gtaaatctcg
     1501 ttcttcgaaa acagttcgcc gccgatattc tgttcgagat tgtcgcgccc cggctcgatg
     1561 cccgcgatca gcgacagggt ttcgctaacg ctcgggacgc ccggaatatc gcgcagcgtc
     1621 cagatgcagc aggcggcgtc gcgcttgtaa tagtcgccgg tcaccatcag cgagaggcca
     1681 tcgaccggtt cgagcttcag cttgccgcgc acaccccatt cgtcgcggcc gttgaagacg
     1741 cgatcgtcgg cgagattctt cacatagccg tcgcgcttgt tgatccggcc cgacagggtg
     1801 aaacccaccc cttccgcgat cgggccgccg atataggcgg cgatccgccg gtcgtcgttc
     1861 ggccaggccc agctaccgct gactttgcct tcgaattcgt cgcccgggtc gcgcgtcgtg
     1921 acattgacga cacccgccga tgcgttcttc ccgaacaagg tcccctgcgg cccgcgtagg
     1981 acctcgatcc gctcgatgtc gggcagatcg gcaaaggatg cccccgactg gccgagcacg
     2041 acgccgtcga cgacgaaggc cgtgctgccc tcgatcgatt ccgaggaaaa ggagctggtg
     2101 ccgataccgc ggacgaaaaa gcccgaattg cgcgtgctcg aggcgttgat gagggtcagg
     2161 ccggggacga catattgcag gtcggtcgcc tcgttcatgg caaaggccgc aagctgttcg
     2221 ggcgacacca ccgagatcga gatcggcacg tcctgcaacc gctcctcgcg cttttgcgcg
     2281 gtgacgacga tctcgtccgc ccgcgacgtg gcttcggcgg ccgcggcttg cgccagtgcg
     2341 gggacactcg cggtcagcgc ggtccccgcg gccagaacgg caagacggcc gcgcgtaaga
     2401 atgttgaaca ttatcgatct cctcccatca ttattttatt atccaaatgg atagtattta
     2461 ttccgagccg ggcgtgcatc gctcgaaagc ccgcttccaa ttcgttcagt cggtcgtccc
     2521 tcgcgcggca atctcgctga ccatctgccg atggcggtcg ggggtgatcg gataaaaacc
     2581 gatcagcagc gcggtcagaa tcccgcccgc gaagggaatc cagaacatca tcgccttgag
     2641 atcggcgagt gtcccgggcg cctgcaccgc atcggcgaca tagccgatat gtgcgagcat
     2701 gaccccgagc agacccgcgc ccaaacccag cgccgccttt tggccgagca tcgtcaagcc
     2761 gaaggcgaag gattcggatc ggattcccgt cttccattcg gcgaactcga ccgtatcggg
     2821 aagcatcgcc cagaagcaga cgtaataggc ggccgatccc atgctcatga ccccgagcgc
     2881 gacgaacagg gcggccgccc cttgcccgtc ggcgagccgc cacaggacca gcccggcaag
     2941 cgacggaacg atgccgatca gccaggcgcg cctcttgccc cagcgtcgcg cgacgagcgc
     3001 gaagaccgga acgaacaccg ccgacacaat cgcgcaaaag cccagtgcgg cgccgccgag
     3061 cgatgcgtcg ccgacgacat atttgaaata atagaggaga ttcttctgaa agatcgtgcc
     3121 cgcgaacgaa ctgaccgcga ccgcgccgag gacgaggaaa agcggccagt tggcggccgt
     3181 cgcgcgcaac ttgtcggcga ccggcggcga caccgcgtcg ctgtgcgtcg gcagatcgag
     3241 cgggcgcacg ccctgcgccg tggcgaggag caccaccgtc gcgatcgcgg catagaggca
     3301 tgcgaccgcc gtccaaccgc tctggccgcc gccgaagagc tccgcgagcg gcagggtcag
     3361 cgtggcaacg agtacgccgg cgagtgtcgc cgacaccatg cgtattcccg tgatatcggc
     3421 gcgcaccgcg gcatcgcgcg tcacgcgcgc tgtgagcgcg gcatagggaa ccgacacgac
     3481 ggcgtagaag gtccggaaca ggaaatgggt cgcccccgcg agcaggacgg cccaggccga
     3541 cggcgaaccg gggacgagga acagcaggac gaaactcgcc gcgagtagcg gagcgccgaa
     3601 gagaagatag gggcgatagc gtccgaagcg gctgcgcgtg cggtcggcgg tcagcccgac
     3661 gatcgggtcg aggaccgcat cccagatcaa agcgaccata tagatgccgc ccgcgacggc
     3721 cggcggcagc ttcaggacgt cggtgtagaa atagaggagg taaaggctgg ccggctgcca
     3781 atagaggttg aacccgaaat cgccgacccc gacggcaagc ttgcgcgaac gggaaagccg
     3841 cgatgccgcc atatccaatt ccatcctcgc catgttgccc gactcatttt cattatccca
     3901 atggatagtg aattgcacga tggcgaacca aatggcaagg gtgattgcga agccgccctc
     3961 tccaacctag gtcttgagcc atgacatcga ccgttcctgc ttccgcaccg ccgcgaccgg
     4021 gcggcaaatc ggcgtccggg cgcagccgcg ccggatcgtc gacccggcaa cagcggctcg
     4081 aacatacccg cgatgcgatc ctggcgagcg cactcgggca tttttcccgg cgcggcttcg
     4141 aaggcgcgtc gctccgcgac atcgcgcagg aggcgggggt tcagcacgga atgatccgtc
     4201 acatctacca aacgaaggaa gaactctggc gccaagccat ctccttcctc tttgaacgga
     4261 tggaaggcga gatgccgcgc gaaatttcgg aggttcatac cggcgaagcg cccgaacgct
     4321 tccgcacctt catccactgg tatgtcggct actgcgcgcg ccaccccgaa cacgcgcggc
     4381 tcatgatcca gcagtcgatt cttgagggcc cggaacttgc ctggaccgcc gaacgctaca
     4441 tccgcggccg gcaccaatgg cacctgccct ttatcgaaaa gctgcaggaa agcggtcatc
     4501 tgccgcaagt cgacccgatc tcgatcgtgc tgatggtgac tgcggcctgt cagatgcctt
     4561 ttcttctcgc tcccgaaatt ctcgccgcca ctggcaagaa ctttctgacg aacgctgcga
     4621 tcgaagcgca cgcagatgcc gtgacgaaga tttttttgcg caaggcggct tgatcgccgg
     4681 tctccgacat aaagccggga ccggtcagac gtccggccgc gctccggacc atttatcgtt
     4741 cgattttggt aaccagaatt accgaggtgg tcgtgcgttc gacgccttcg aacaatccga
     4801 catcgtcgat cagctcgttg agctgtgcgc tgtcctcggc ctcaagcatc gcgatgatat
     4861 catattcgcc gctgatcgcg tggatcgcct gcaccagagg aagcgcggcg agcgcctgct
     4921 cgacctcgcg gtgaaagcgg ggcagagtct tgatcatcat atgcgcgcgc acccggctcg
     4981 cggcaaaggc gttgccgagc cgcaccgtat agccggcgac tatcttatcc tcttccagcc
     5041 gcgcgagacg cgaatagatc gctccgcgcg acaggccgag ttccttcgcg agttgcgcga
     5101 tcgtctggcg ggcatcatcg cgcagcaggg tgagcaggcg ctcatcgatc tcgtcgagat
     5161 tggcgccctg atgtggcatc aatcggccaa tttctcgtcg gcgaagatcg cggtctgtgg
     5221 cccctcccct gcgctcatcc gcccgcgata catgcccagg tcgttgatgg caaaggcggg
     5281 gcgtccgtcg gggcccatcg cgatcagccc gccgtcaccg ccgatcgcgc cgaccgccat
     5341 gatcgcgcct tgcgccgcgt ccttcaagct ctcgcccttc catgcgacgc ggtcgcacac
     5401 ttgccgggcg gcgctttcgc ggatgaaata ttcgcccgac ccggttgccg acacggcgca
     5461 ttggccattc ttggcatagg tgcccgcgcc gatgatcggg gagtcgccga tgcggcccca
     5521 tcgcttaccc gtcatcccgc cggtcgaggt cgctgcggcc agattgccct cggcgtcgag
     5581 ggcgaccgca ccgacggtgc cgaacagatg cgtcggatcg actgccgctt gattcttctt
     5641 tcgccagttc agaagctgct gccaacgctc ctcggtgcgg aaccaggcgg gttcgacctg
     5701 ttcgaggccc tgttcgaccg aaaagcgatc ggcgccatcg cgtgccagca tgacgtgcgg
     5761 actcttgtcc atcaccgcgc gcgcgaggtc gatcggatgg cgcgtgcgcg tcacccccgc
     5821 gaccgcgccg gccttttgcg tcttgccgtc catgatcgcg gcgtcgagtt cgttcctgcc
     5881 ctcggcggtg aacaccgcgc cgcggccggc gttgaatatg gggttatcct cgagcacgcg
     5941 caccgccgcg gcgaccgcat cgtgcaccga cgcgtcggcc ccttccccac cgctcggggc
     6001 caccgagaac gccaccagca tgattccccc ttcgacggga tggacgggtc cctcgatgat
     6061 gtcatcttc
//
LOCUS       DATMSU010000389         7182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_120809,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000389 DATMSU010000000
VERSION     DATMSU010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7182
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120809"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VN034_10450"
     CDS             <1..696
                     /locus_tag="VN034_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWV61065.1"
                     /translation="AGATAQTLAGLQDLGDRGHALAATMTRIVDDGSRARAVRGDAHI
                     KIKQASERAAALKAASQSIEQMLALIIDMASRTNMLALNAGIEAARAGDAGRGFAVVA
                     SEVKTLASQTRVAAGDITQYIDRIRDIVAQVAAGFDEVEKAIDANNGFSDAIDRAVDG
                     QSATTLTIASYVEQAVFAGREIDARVREIGHGATAVGDGARTLGRLSAGLTEAAQSLH
                     QRAQHFVAAVAIA"
     gene            796..1521
                     /gene="aqpZ"
                     /locus_tag="VN034_10455"
     CDS             796..1521
                     /gene="aqpZ"
                     /locus_tag="VN034_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HWV61066.1"
                     /translation="MTNIQKGLAEFIGTFWLVFGGCGSAVLAAAFPDVGIGLLGVSLA
                     FGLTVVTMAYAIGHISGCHLNPAVTVGLWAGGRFDARDIPLYVIAQVLGAIVAAFLLF
                     YIASGSPVYDLATNGLAANGFGEGSPGGYDIWSGVLIEVVLTAFFVWIIMGSTDGRAP
                     AGFAPLAIGLALALIHLISIPVTNTSVNPARSTGPALVVGGLAFQQLWLFWVAPLIGG
                     AIGGLLYKMLGADTFPKPNIEGE"
     gene            complement(1556..2419)
                     /gene="galU"
                     /locus_tag="VN034_10460"
     CDS             complement(1556..2419)
                     /gene="galU"
                     /locus_tag="VN034_10460"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWV61067.1"
                     /translation="MKPIRKAVFPVAGLGTRFLPATKAIPKEMLPVVDRPLIQYAVDE
                     AREAGIEQMIFVTGRGKSAIEDHFDVAFELEKTMSERGKDLSVLEPTRLGPGNCAYVR
                     QQEPLGLGHAIWCARDIVGDEPFAIFLPDEFMHGSPGCMKQMVDAYSRVGGNLISVLE
                     VPREQVSSYGVIAPGARDGALTEVTGLVEKPAVDAAPSNLIISGRYILQPEVMRVLER
                     QEKGAGGEIQLTDAMATMIGDQPFHAVTFDGARYDCGSKAGYIQANLAVALERPDMAD
                     EVRAFAIDLLK"
     gene            complement(2480..2977)
                     /locus_tag="VN034_10465"
     CDS             complement(2480..2977)
                     /locus_tag="VN034_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV61068.1"
                     /translation="MTYAFPHNIRLFAIAVLAALLAACQSAPTADGFSAAQIATLRSE
                     GFVDTGLGWELSMPERLLFPSNQSSVPADQQKRITDIAAKLVSVGINTARVEGHTDST
                     GTSAYNLWLSQARAEAVATPMQAGGMRFTADQIVGRGETLPLSSNETPEGRQDNRRVV
                     VIVTP"
     gene            complement(2994..4220)
                     /locus_tag="VN034_10470"
     CDS             complement(2994..4220)
                     /locus_tag="VN034_10470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWV61069.1"
                     /translation="MSRPDLPRPAQRIGARTTLQKLLSRFHFGITLFAVALSGLTILL
                     AGVTALRGYADRNMELAAQLGAYGVEPALVFNDPVAVREGLEPLTRIPGIARLRVLND
                     VGRPVTEWTSPTADPAPVLTNIFFPRPFAVTVERNGSAIGTIEVWGDSSTLIDYVRLG
                     LLAGLACLLITAFGTIFLARRFEYELVKPLNEIATVAHDVRLHRRFDKRVQPLGIAEL
                     DRLGGDINALLDELQGWQGHMESEHALLAHRASHDPLTAMPNRAAFDEQLARRIDAAA
                     ARGSRFALLFIDADNFKAANDNFGHAAGDAVLVALSACIKETLRKNDFAARIGGDEFV
                     VIIDPFDVDAVADELASRIRTCVSQPIALPDGQAYRASVSVGVAVFPDSGSNATALLT
                     AADAAMYADKLANRSR"
     gene            complement(4220..4756)
                     /locus_tag="VN034_10475"
     CDS             complement(4220..4756)
                     /locus_tag="VN034_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HWV61070.1"
                     /translation="MLASLFLVGALVMTPQMSVQAAGQSVVEQSAGGMTRAVNRMIGG
                     IASYARWPDGSAAANRRMCVVGTPRLAPRMAPDTPGRGSISVHLTPAAGVTPDCDILF
                     LGRMPVADRRQLIAWVRGRPVLTITDDDAACIYGAMFCLNNRAANLSFSVNLDAIGRG
                     PLRIDPRVLRIGSGQGGA"
     gene            complement(4893..5429)
                     /gene="msrA"
                     /locus_tag="VN034_10480"
     CDS             complement(4893..5429)
                     /gene="msrA"
                     /locus_tag="VN034_10480"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWV61071.1"
                     /translation="MTRETAIFAGGCFWCTEAVFQSLEGVDGVESGYIGGEVANPTYK
                     QVCGGDTGHAEAIRIGFDPSVISYADLLDVFFATHDPTQLNRQGNDIGTQYPSAIFPL
                     DAAQADAARAGIARAAGDWPAPIVTTVESASDWYPAEDYHQAYWEGEGQRNPYCMAVI
                     PPKLAKLRKGFSERLRTA"
     gene            complement(5486..6475)
                     /locus_tag="VN034_10485"
     CDS             complement(5486..6475)
                     /locus_tag="VN034_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWV61072.1"
                     /translation="MPHRARSILAVLMLSISVPSMAAPAEPSPYAALAAAEARVAAIG
                     FRLTTENAAWCPVRQPQFGWIWGDPRLYDADRRAEALAAYGAVERDAAFVAAVAPGSP
                     AARTGLHVGDSVAWVDMTPVANGIGDHPFARITALEQRLAALPTDAAIWLGVTDGRTV
                     PLTPVAGCVSDFRVEANEKPGAVADGRMVLVNQGLADFAPDDAELAAAIAHELAHNIL
                     RHRARLDAAGVDRGLAKQFGRNARMFKQTEIEADRLSVWLLAGAGYDPAAAARFWSRF
                     GQRKGRPMFQASTHPSWRDRVAALEAEAATIAAARMLGRPLHPPLIEAPPPLE"
     gene            6581..>7182
                     /locus_tag="VN034_10490"
     CDS             6581..>7182
                     /locus_tag="VN034_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWV61073.1"
                     /translation="MTQISTSPAPTTRQSRPAALARWLWAVALLVIIVVAVGGITRLT
                     ESGLSITEWRPVSGVIPPLTEAGWIEEFEKYKRIPEYKAINLGMTLEGFKAIFFWEWL
                     HRILGRLVGMALVVPLIWYAWRRAIPAGYGWRLFALAALVGLQGAIGWWMVASGLEYR
                     TDVSHFRLATHLLTALFLLAGLVWTARDLGALARDPAARPA"
BASE COUNT         1160 a         2343 c         2459 g         1220 t
ORIGIN      
        1 gcgggggcga cggcgcagac gctcgccggg ctgcaggacc tcggcgatcg cggccatgcg
       61 ctggccgcga cgatgaccag gatcgtcgac gacggatcgc gggcgcgggc ggttcgcggc
      121 gatgcgcata tcaagatcaa acaagccagc gaacgcgcgg cggcgctgaa agcggcgagc
      181 cagtcgatcg agcagatgct cgcgctgatc atcgacatgg cgagccgcac caatatgctc
      241 gcgctcaacg ccgggatcga ggctgcgcgc gcgggcgacg ccggacgcgg tttcgccgtg
      301 gtcgcctccg aagtgaagac gctggccagc cagacgcggg tcgcggcggg cgacatcacc
      361 caatatatcg accggatccg cgacatcgtc gcgcaggtcg ccgcgggttt cgacgaggtg
      421 gaaaaggcga tcgacgcgaa caacggcttc tccgacgcga tcgaccgcgc ggtcgatggc
      481 cagagcgcga cgacgctgac gatcgcgagc tatgtcgagc aagctgtgtt tgcggggcgc
      541 gagatcgacg cgcgcgttcg cgagatcggc cacggcgcca cggcggtggg cgatggcgcc
      601 cggacgctcg gccgactgtc ggctgggctt accgaggcgg cgcagtcgct gcaccagcgc
      661 gcgcagcact tcgtcgcagc ggttgccatc gcctgattcc gcgcgctcgg acaagggctt
      721 gcccgattcc gcaaccttga taggatgacc ccgcgccgga ggcgtcagag gccgccggcg
      781 gggaggaggg tcatcatgac gaatatccaa aagggcttgg ccgaattcat cggcacgttc
      841 tggcttgtgt tcggcggctg cggcagcgcg gttctggccg ccgcctttcc cgatgtcggc
      901 atcggattgc tcggcgtttc gctcgctttc ggcctgacgg tcgtgaccat ggcctatgcg
      961 atcggccata tttcgggctg tcatctcaat cccgcggtca ccgtcggcct gtgggcgggc
     1021 gggcgtttcg acgcgcgcga catcccgctc tatgtgatcg cacaggtgct tggcgcgatc
     1081 gtcgcggcgt tcctgctttt ctatatcgcc agcggcagtc ccgtttatga cctcgccacc
     1141 aacggcctgg cggcgaacgg cttcggcgaa ggatcgcccg gcggttacga catctggtcg
     1201 ggcgtgctga tcgaggtcgt gctgaccgcc ttcttcgtct ggatcatcat gggatcgacc
     1261 gacgggcgcg cgcccgcggg ctttgcgccc ttggcgatcg gccttgcgct ggcgctgatc
     1321 cacctcatct cgatcccggt gaccaacacg tcggtcaacc cggcgcggag caccggtccg
     1381 gcgctcgtcg tcggcgggct ggcgtttcag caattgtggc tgttctgggt cgcgccgctg
     1441 atcggcgggg cgatcggggg gctgctctac aagatgctcg gggccgatac cttccccaag
     1501 ccgaatatcg aaggcgaata atcgccttcc cggcggaggc gccgcctccg ccggattatt
     1561 tcagcaggtc gatcgcaaag gcgcggacct cgtccgccat atcggggcgt tcgagcgcaa
     1621 ccgccagatt ggcctggata tagccggcct tcgatccgca atcgtaacgc gcgccgtcga
     1681 aggtgacggc gtggaagggc tggtcgccga tcatcgtcgc catcgcgtcg gtgagctgga
     1741 tttcgccgcc ggcgcccttt tcctggcgtt cgagcacgcg catcacttcg ggctgaagga
     1801 tatagcggcc cgagatgatc aggttcgacg gcgcggcgtc gaccgccggt ttttcgacaa
     1861 ggccggtcac ctcggtcagc gcgccgtcgc gcgcaccggg cgcgatcacg ccataggagg
     1921 acacctgttc gcgcggaact tcgagcaccg agatgaggtt gccgccgacc cgggaatagg
     1981 cgtcgaccat ctgcttcatg cagccgggcg acccgtgcat gaattcgtcg ggcaggaaga
     2041 tcgcgaacgg ctcgtcgccg acgatgtcgc gcgcgcacca gatcgcgtgg cccagcccca
     2101 gcggctcctg ctgccggaca taggcgcaat tgccgggacc cagccgcgtc ggttcgagca
     2161 ccgacagatc cttgccgcgt tcggacatcg tcttttcgag ttcgaaggcg acgtcgaaat
     2221 gatcctcgat cgcgctcttg ccgcgcccgg tgacgaagat catctgttcg atcccggcct
     2281 cgcgcgcttc gtcgacggca tattggatca gcggccggtc gacgacgggc agcatttctt
     2341 tcggaatcgc cttggtcgcg ggcaggaagc gcgtgccgag gccggcgacg ggaaagacgg
     2401 ctttgcggat cggtttcatg gacgaaggcc tagcggcgaa cgccggccaa ggaaaggcag
     2461 caggtcgctc gccgcgctat tacggtgtga cgatcacgac cacgcggcgg ttgtcctgcc
     2521 gtccctcggg tgtctcattg ctcgacaagg gcagggtttc gccgcggccg acgatctgat
     2581 ccgcggtgaa gcgcatcccg ccggcctgca tcggcgtcgc gaccgcttcc gcgcgcgcct
     2641 gcgacagcca aagattatag gccgaggttc cggtcgaatc ggtatgtcct tcgacccgcg
     2701 cggtgttgat cccgaccgag acgagcttgg ccgcgatgtc ggtgatccgc ttttgctgat
     2761 cggcgggcac gctgctctgg ttcgacggaa aaagaagccg ttcgggcatg ctcaactccc
     2821 agccaagtcc ggtgtcgacg aaaccttccg accgaagcgt cgcgatctgt gccgcgctga
     2881 agccgtcggc ggtcggcgcg ctctggcacg ccgcgagcaa cgccgccaaa accgcgatcg
     2941 cgaagaggcg gatattgtgg gggaaggcgt aagtcatggc gtctctccgg tggtcatcgg
     3001 gaacggttgg ccaatttgtc cgcatacatc gcggcgtcgg cggcggtaag tagtgcggtc
     3061 gcgttgctgc cgctgtccgg gaaaaccgcg accccgacgc tgaccgacgc gcgataggcc
     3121 tgcccgtccg gcaacgcgat gggttgcgac acgcacgtcc ggatccggct ggcaagctca
     3181 tcggcgacag cgtccacgtc gaaggggtcg atgatgacca cgaattcgtc gccgccgatg
     3241 cgggctgcga aatcattctt gcgcagcgtt tccttgatgc aggccgacag tgcgacgagc
     3301 acggcgtcgc ccgcggcgtg gccgaaattg tcgttggccg ccttgaaatt atcggcgtcg
     3361 atgaacagca gcgcaaagcg gctgccgcgt gccgcggcgg cgtcgatgcg ccgcgcgagc
     3421 tgctcgtcga aggccgcgcg gttgggcatc gcggtcagcg ggtcgtgcga cgcgcggtgg
     3481 gcgagcagcg cgtgttcgct ttccatatgg ccttgccagc cctgcaattc gtcaagcagg
     3541 gcattgatgt cgccgcccag ccggtcgagt tcggcgatgc cgagcggctg gacccgcttg
     3601 tcgaagcggc ggtgcagtcg cacgtcatgc gcgacggtcg cgatttcgtt aagcggcttg
     3661 acgagctcat attcgaaccg ccgtgcgaga aaaatcgtgc cgaatgcggt gatgagcagg
     3721 caggccagcc cggcgagcag accgagccgg acatagtcga tgagcgtcga actgtcgccc
     3781 catacctcga tcgttccaat cgccgatccg ttgcgctcga ccgtcactgc gaagggacgg
     3841 gggaaaaaga tgttcgtgag cacgggcgcc ggatcggcgg tgggcgacgt ccattcggtc
     3901 accggccggc cgacatcgtt gagcacccgc agccgcgcga tgccggggat gcgagtgagc
     3961 ggttcgagcc cttcgcgtac ggcgaccgga tcgttgaaca ccagcgccgg ctcgacgcca
     4021 taggcgccga gttgcgctgc cagttccatg ttgcggtcgg catagccgcg cagcgccgtc
     4081 accccggcga gcaggatcgt cagcccggag agtgcgaccg cgaatagcgt gatgccgaaa
     4141 tggaaacgcg acagcagctt ttgcagcgtc gtgcgcgcgc cgatgcgttg ggcggggcgc
     4201 ggcagatcgg gtctgctcat catgcgcctc cctgacccga accgatgcgc agcacgcgcg
     4261 ggtcgatacg cagcggcccg cggccgatcg cgtcgagatt gaccgagaag ctgaggttgg
     4321 ccgcgcgatt gttgaggcag aacatcgcgc catagatgca cgccgcgtca tcatcggtga
     4381 tcgtcaggac cggacggccg cgcacccatg cgatcaattg ccgccggtcg gcaaccggca
     4441 tccggccgag gaacaatata tcgcaatcgg gggtgacgcc ggctgcagga gttagatgga
     4501 ccgagataga gcctcggcct ggcgtatccg gcgccatgcg gggcgcgagg cgcggcgtcc
     4561 cgacgacgca catccggcgg tttgctgcag ccgacccgtc gggccagcgg gcgtagctgg
     4621 cgatgccgcc gatcatccgg ttgaccgccc gcgtcatccc gccggcgctt tgctcgacaa
     4681 ccgactgacc tgccgcttga accgacatct gcggcgtcat caccaacgcg ccgaccagaa
     4741 acagtgatgc cagcaccgcg cgcccccatc ttcgtttcag ggcccaccct gccccagcga
     4801 aggggcgtcc gctagtcaaa acaattggaa agtcaatgaa aagtgacgtc atgcggccat
     4861 attgcaacat ggccgctttt gtcgtgaccc cgctatgcgg tgcgcaagcg ctcgctgaaa
     4921 cccttgcgca atttggccag tttcggggga atgaccgcca tgcaataggg gttgcgctgt
     4981 ccttcgcctt cccaataggc ctgatgataa tcctcggccg gataccagtc ggacgcgctt
     5041 tcgaccgtcg tgacgatcgg cgcgggccag tcgcccgcgg cccgcgcgat tccggcgcgc
     5101 gccgcgtcgg cctgcgcggc gtcgagcggg aagatcgcgc tgggatactg ggtgccgatg
     5161 tcattgccct gacggttgag ctgcgtcgga tcgtgcgtcg cgaaaaagac gtcgagcagg
     5221 tcggcatagg aaatgaccga ggggtcgaag ccgatgcgga tcgcctcggc atgtccggtg
     5281 tcgccgccgc acacctgctt gtaggtcggg ttcgcgactt cgcctccgat atagccgctc
     5341 tcgacgccgt cgacgccttc gagcgactgg aacacggctt cggtgcacca gaagcagcct
     5401 ccggcgaaaa tggcggtttc acgggtcatg gcgtcgatcc ttgtcgggga gaatggaatg
     5461 tcttccaaat aggatgcgcc gcatcctatt ccaagggcgg cggcgcttcg atcagcggcg
     5521 ggtgcagcgg ccggccaagc atccgcgccg cggcgatagt ggcggcttcg gcttcgagcg
     5581 cggcgacgcg gtcgcgccac gagggatggg tgctcgcctg gaacatcggc cggcccttgc
     5641 gctggccgaa acgcgaccag aagcgcgccg ccgcggcggg atcatagccc gccccggcga
     5701 gcagccacac cgacaggcga tcggcctcga tctcggtctg cttgaacatg cgcgcgttgc
     5761 ggccgaactg tttcgccagc ccgcgatcga cccccgccgc gtcgagccgc gcgcggtggc
     5821 gcaggatatt gtgcgccagt tcgtgcgcga tcgccgccgc gagctccgcg tcgtcgggcg
     5881 caaaatcggc gaggccctga ttgacgagca ccatgcgtcc gtcggcgacg gcgccgggtt
     5941 tctcgttcgc ctcgacgcga aagtcgctga cgcacccggc cacgggggtc agcgggacgg
     6001 tccgcccgtc ggtaacgcct agccaaatcg cagcgtcggt cggcagggcg gccagccttt
     6061 gttcgagcgc cgtgatgcgc gcgaagggat ggtcgccgat gccattcgcg accggcgtca
     6121 tgtcgaccca ggcaacactg tcgcccacat gcaaaccggt ccgagcagcg ggcgagcccg
     6181 gtgcgaccgc cgcgacaaag gctgcatccc gttcgaccgc cccataagca gcgagcgcct
     6241 cggcgcgccg gtccgcgtcg taaaggcgcg ggtcgcccca gatccagccg aactgcggct
     6301 ggcgcaccgg gcaccaggcc gcgttttcgg tcgtcaggcg aaatccgatc gccgccacgc
     6361 gcgcctcagc cgccgccagc gcggcatagg gcgatggctc ggcgggcgcc gccatcgacg
     6421 gcacgcttat cgacagcatc aggacggcca ggatcgatcg agcgcggtgc ggcatcggcc
     6481 tttccctatc aaggcacacc tgtcgcgacc atgaatttcc ccggatggtc gggctgggga
     6541 ccccttgcct atcgtcctac gagcgccgat agggcgggcg atgacgcaaa tttccacctc
     6601 gcccgcgccc actacgcgcc agtctcgccc cgccgcgctg gcgcggtggc tctgggcggt
     6661 cgcgctgctc gtgatcatcg tggtcgcggt cggcggcatc acccgcctga ccgaatccgg
     6721 cctgtcgatc accgaatggc ggccggtttc gggcgtgatc ccaccgctga ccgaggccgg
     6781 atggatcgag gagttcgaga aatacaagcg gattcccgaa tataaggcga tcaacctcgg
     6841 tatgacgctc gagggcttca aggcgatctt cttttgggaa tggctgcacc gcatcctcgg
     6901 gcggttggtc ggcatggcgc tcgtcgtccc gctgatctgg tacgcgtggc gccgcgctat
     6961 cccggcggga tatggctggc gcctgtttgc gctcgccgcg ctcgtcgggc tgcaaggcgc
     7021 gatcggctgg tggatggtgg cttcggggct cgaatatcgc accgacgtca gccattttcg
     7081 tctcgcgacg cacttgctga ccgcattgtt cctgctcgcg ggactcgtct ggacggcgcg
     7141 cgacctcggc gcgctggcgc gcgatcccgc cgcgcggccg gc
//
LOCUS       DATMSU010000390         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_139510,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000390 DATMSU010000000
VERSION     DATMSU010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139510"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1756)
                     /locus_tag="VN034_10495"
     CDS             complement(167..1756)
                     /locus_tag="VN034_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWV61074.1"
                     /translation="MIGEVVDISGSASRILLDSAVIGKLASSSDAAVAMAGQVGAQVK
                     VRVGNVWLIASIRDQQLHERGDGLIVATIDFLGEGEEEKITGRIHNFRRGVTRYPIPG
                     SQVFAATSADLKQIYAADERAHVEIGTVYPTKDIRGSLYVDAMLGKHFALLGSTGTGK
                     STSAALILHKICELAPQGHIVMIDPHGEYSAAFKGTGALFDVDNLAMPYWLMNFEEHC
                     EVFVTAEGRDRQADCDVLARCLLQARTKNRLAEGMTKITVDSPIPYLLSDLLNILQNE
                     MGKLDKATSSAPFMRIKGKIEEMRADPRYNFMFSGMLVADTMANFLGKIFRLPSDGRP
                     ISIIDVSGVPSDITGVVVAVLSRLTFDYAIWSRGEPQRPILLVCEEAHRYIPAKDVGT
                     GQAVRKILERIAKEGRKYGVSLGLITQRPSDLAEGVLSQCGTIISMRLNNERDQHFVK
                     AAMPEGARGFIDSIPALRNRECIICGEGVSIPIRVHLDTLEEEKRPASSDPLFSKLWR
                     ETGGEAEILERVVKRWRSQGR"
     gene            complement(1978..2751)
                     /locus_tag="VN034_10500"
     CDS             complement(1978..2751)
                     /locus_tag="VN034_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HWV61075.1"
                     /translation="MTAVRALILAAALALFPATAAHAADPRLVPDVSSRAIDIQYSFT
                     GEELLLFGAILYPGQRLPDDRADIVVVLKGPVRPIVLREKRRVAGIWVNANSIRLRTS
                     PGFYAIGSSRPIDKLVDERTAAIFELGLANLSMSPTGFPEAKTLERFEAGLIDLYRRA
                     GLFVENPASVEITEGVLYRARIPVPARVPVGTYRAETYLISRGRVLAVASRDVQIRKA
                     GFERFVALAAERHGFLYGLSAVLLSLVLGYGASAIFRRR"
     gene            complement(2748..>3224)
                     /locus_tag="VN034_10505"
     CDS             complement(2748..>3224)
                     /locus_tag="VN034_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWV61076.1"
                     /translation="PAPARKRRHHPMVANLPLRWRFYRSGLYISPLAPLILGMVVGIL
                     TMLLGVGGGFIMVPAMIFLLGMGTQVVVGTSLFQILFVTIATTMVHAMTTGAVDIVLA
                     GLLLLGSVTGAQIGARFAQRVKPEYLRMALAVIVLLVAARMAVDLFIRPDEIYTVQ"
BASE COUNT          588 a         1045 c         1066 g          525 t
ORIGIN      
        1 aacgaccgcg ccgccgcctg ccgcggcgcc gacctatacc gatccgcggc tcaaccaatc
       61 aggccagatc cgcgaggaat tccgcaagag cggggaatgg aaaggctgag gttttcggca
      121 cgaagcgcgg catcgcgctt ctttcgttta cgggaattgc aggcggttac cgcccctggc
      181 tgcgccaccg cttgacgacg cgttcgagga tttccgcctc gccgccggtt tcgcgccaca
      241 gcttggagaa gagcggatcg ctcgacgccg gccgcttttc ctcttcgagc gtgtcgaggt
      301 ggacgcggat cgggatcgag acgccttcgc cgcagatgat acattcgcgg ttgcgcaaag
      361 cggggatcga atcgatgaag ccgcgcgcgc cttcgggcat cgccgctttc acgaaatgct
      421 ggtcgcgctc gttgttgagg cgcatcgaga tgatcgtgcc gcactgcgac agcacgcctt
      481 cggcaaggtc cgaggggcgc tgggtgatca ggcccagcga cacgccatat ttgcggcctt
      541 ccttggcgat gcgttcgagg atcttgcgca ccgcctgtcc ggtcccgacg tccttcgccg
      601 ggatgtagcg gtgcgcttcc tcgcagacga gcaggatcgg gcgctgcggt tcgccgcgcg
      661 accagatcgc atagtcgaag gtcagccgcg acagcaccgc gacgacgacc ccggtgatgt
      721 ccgacggcac gcccgacacg tcgatgatcg agatcggccg cccgtccgac ggcaggcgaa
      781 agatcttgcc gaggaaattc gccatcgtgt cggcgacgag catgcctgag aacatgaaat
      841 tatagcgcgg atcggcgcgc atttcctcga tcttgccctt gatccgcatg aagggcgccg
      901 acgaggtcgc cttgtcgagc ttgcccatct cgttctggag gatgttgagc aggtcggaga
      961 gcagataggg gatcggcgag tcgaccgtga tcttggtcat gccttccgcc agccggttct
     1021 tggtccgcgc ctggagcaga cagcgcgcga gcacgtcgca gtcggcctgg cggtcgcgcc
     1081 cttcggcggt gacgaacacc tcgcaatgtt cctcgaaatt catcagccaa tagggcatcg
     1141 ccagattgtc gacgtcgaac agcgcgccgg tgcccttgaa ggcggccgaa tattcgccgt
     1201 gcggatcgat catcacgata tggccctgcg gcgcgagctc gcagatcttg tggaggatca
     1261 gcgcggcgct cgtcgacttg ccggtgccgg tcgagccgag aagcgcgaaa tgcttgccga
     1321 gcatcgcatc gacatagagc gagccgcgaa tatccttggt cggatagacg gtgccgatct
     1381 cgacatgcgc gcgctcgtcg gcggcgtaaa tctgcttgag gtcggcgctg gtcgccgcga
     1441 acacctggct gcccgggatc gggtagcgcg tgacgccgcg gcggaaatta tggatgcggc
     1501 cggtgatctt ttcctcttcg ccttcgccga ggaaatcgat cgtcgcgacg atcagcccgt
     1561 cgccgcgttc gtgcagttgc tggtcgcgaa tactcgcgat cagccagaca ttgccgacgc
     1621 ggaccttgac ctgcgccccg acctgtcccg ccatcgccac cgccgcgtcg ctcgacgacg
     1681 ccaattttcc gatcactgcg gaatcgagca ggatgcgcga ggccgaaccc gaaatatcga
     1741 cgacttcgcc gatcatcagg tcgtcgcgat gatggttggc taccggcacg accggcgccg
     1801 ccacgccgcc gccggtcaga cgcacatgct gcgccgccgt caaatccccg gcgccgaatc
     1861 cgccaccctg catgtccata tatccgcccc gcctattgtg gtcccggccc cagctatcgc
     1921 agacgagggt aaataggccg tgaacttagg actaaaagct tgtggtcgag acaggctcta
     1981 gcggcggcgg aaaatcgccg acgcgccata gccgagcacg agcgacagga gcaccgcgga
     2041 gaggccgtag agaaagccgt ggcgctccgc cgcgagcgcg acgaagcgtt cgaagcccgc
     2101 tttgcggatc tggacgtcgc gcgatgcgac cgcgagcacg cggccgcggc tgatcaaata
     2161 ggtttcggcg cgataggtcc ccaccggaac gcgcgccggc acgggaatgc gggcgcgata
     2221 gagcacgccc tccgtaatct cgaccgaagc gggattttcg acgaagaggc ccgcccggcg
     2281 ataaaggtcg ataagccccg cttcgaaccg ctcgagcgtc ttcgcttccg gaaatccggt
     2341 cggcgacatc gacagattgg ccaggcccag ctcgaagatc gccgcggtcc gttcgtcgac
     2401 cagcttgtcg atcgggcgcg acgatccgat cgcatagaag ccgggcgagg tgcgcagccg
     2461 gatgctgttc gcattgaccc atattcccgc gacgcggcgc ttttcgcgca acacgatggg
     2521 ccgcaccggc cccttcagca cgacgacgat gtcggcgcgg tcgtcgggca ggcgctggcc
     2581 gggatagagg atcgcgccga acagcagcag ttcctcgccg gtgaagctgt actggatgtc
     2641 gatcgcgcgg ctcgacacgt cggggacgag ccgcggatcg gcggcgtgcg ccgcggtcgc
     2701 gggaaacagc gccagcgcgg ctgcgaggat caaggcgcgc accgccgtca ttgcaccgta
     2761 tagatttcgt cggggcggat gaagaggtcg accgccatgc gcgccgcgac gagcagcacg
     2821 atgaccgcca gcgccatgcg cagatattcg ggcttcaccc gctgggcaaa gcgcgcgccg
     2881 atctgcgccc cggtaacgct gccgagcagc agcagcccgg cgagcacgat gtcgaccgcg
     2941 cccgtcgtca tcgcgtgaac catcgtcgtc gcgatcgtca cgaacaggat ctggaacagc
     3001 gaggtgccga cgaccacctg cgtccccatg ccgagcagga agatcatcgc gggcaccatg
     3061 atgaagccgc cgccgacgcc gagcagcatc gtcaatatgc cgaccaccat gccgaggatc
     3121 agcggcgcga gcggcgaaat atagaggccc gagcgataga agcgccagcg cagcggcagg
     3181 ttggcgacca tcgggtggtg gcgccgcttg cgcgcgggcg cggg
//
LOCUS       DATMSU010000391         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_92357, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000391 DATMSU010000000
VERSION     DATMSU010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92357"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VN034_10510"
     CDS             <1..276
                     /locus_tag="VN034_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HWV61077.1"
                     /translation="VVARGRGDVALSIRELARAASVPMLEYPQLTRAIYFTARAGRVI
                     PEELFVAVATVLAFVFQLERAVADGLTQPAVDIPPSHRFDPEGRRQA"
     gene            321..1691
                     /gene="fliD"
                     /locus_tag="VN034_10515"
     CDS             321..1691
                     /gene="fliD"
                     /locus_tag="VN034_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HWV61078.1"
                     /translation="MVSSIANSLGFGSGLDVKQLVTDLSNASRDPKIARMADLTQANQ
                     TRISALAQARSDLDGFADSLSQMVNDGTLRSTPTVSDESVLSATSRAGLSADSFAATV
                     VVNQLARAQTNYSGVVADKAAAIGAGTMTLTVGGVAKTITVDATNNSLDGLAKAINAS
                     GAGVTASIIADEGGHRLILKGPTGEAGAFTLAADAGADPGLSAFATGGGMTEGQSAAN
                     AEFTIDGVAFSRATNIIDDVVPGMSLTLKKAAPGQPVDIGASRPLDMIKQTVGDFVAV
                     YNQLKKSLVAASSLSGPTTGLRELERELGGLIHQVVSSHGSINQLSDIGVSITKEGLL
                     SVDNKKLDKALESDAGAVEALFNPRRDATHTEQSDPGIAFALDAIRDKAVGTNGAIDR
                     VSKSLDTKKENLADQLEKIEEREDAYRKRLEKQYGSLEAKLAAFKATQSYLEQQIKLW
                     SNQGND"
     gene            1714..2103
                     /locus_tag="VN034_10520"
     CDS             1714..2103
                     /locus_tag="VN034_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HWV61079.1"
                     /translation="MTATASTVRATGLYRRLQNESRAAAADPVELVTMLYDELETAVG
                     VLAAMVRQGQRISATEPAHRARAILIGLDVNLDRDKGGDVATALSRVYRSMRRKLDDA
                     VAANSAEALSELLDGILTISAAWRQLR"
     gene            complement(2294..3367)
                     /locus_tag="VN034_10525"
     CDS             complement(2294..3367)
                     /locus_tag="VN034_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWV61080.1"
                     /translation="MTLGHNENTALEALIIGSSPATCRLREMIRRVARSNASVMLCGP
                     SGSGKELVARAIHDEGVRAGKAFSAINCGAIPGELIESELFGHEKGSFTGAHARRIGH
                     FEASEGGTLFLDEIGDMRFDMQVKLLRVLEDRTIVRVGSSEVKAVDVRVISATHQDLG
                     AAIADGKFREDLFFRLGVVVLQVPSLASRVEDIPALVRHFQRQMPADAKCRYDDAAMA
                     LLMQHAWPGNVRELRNFVERASVLHGGEALGADDVAMLLNPTAALAPRLAVPSSPAAV
                     QASADDFAAAPFASAAPTAKTPAPGRPIDLKREIETIELEQIHVALDLADGIISEAAR
                     LLTLKRTTLIEKMRKYGVHQQAA"
     gene            complement(3549..>4114)
                     /locus_tag="VN034_10530"
     CDS             complement(3549..>4114)
                     /locus_tag="VN034_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV61081.1"
                     /translation="TGTRAMTIPRDAVGGPREASGREREAESKKFNLLAQSLKERLQS
                     RADLKRLARNLRFTETMEGLRIDIVDDADVSMFAIGTSQLTPSGAKLFGEVAALVAEV
                     PNRVMVRGHTDAAPWSAKSGTNNWRLSVDRAEVTRHYLEFRGIASERFSRIEGVADRE
                     PYVPSDRFDPRNRRISITLGWRGAGEN"
BASE COUNT          739 a         1389 c         1323 g          663 t
ORIGIN      
        1 gtcgtcgcgc gcgggcgcgg cgacgtcgca ctgtcgatcc gcgagctggc gcgcgccgcc
       61 agcgtcccga tgctcgaata tccgcagctc acccgcgcca tctatttcac agcgcgcgcg
      121 ggccgcgtga tccccgagga attgttcgtc gcggtcgcga ccgtgctcgc cttcgtcttc
      181 cagctcgaac gcgcggtcgc cgacggcctg acccagcccg cggtcgacat tccaccctcg
      241 caccgtttcg atcccgaggg acgccgccag gcttaatctt gtccgcgcgc cgccgttaag
      301 cagtgcgaag gatatttgcc atggtcagtt caatcgccaa cagcctcggt ttcggctcgg
      361 gcctcgacgt caaacagctc gtcaccgacc tgtcgaacgc gtcgcgcgat cccaagatcg
      421 cgcgcatggc cgacctgacg caggcgaacc agacgcggat cagcgcgctc gcgcaggcgc
      481 gatccgacct cgacggattc gccgattcgc tgagccagat ggtcaacgac gggacgctgc
      541 gcagcacgcc gaccgtgtcc gacgaaagcg tgctcagcgc caccagccgc gcgggccttt
      601 ccgccgacag ctttgccgcg accgtggtcg tgaatcagct cgcgcgcgcg caaaccaatt
      661 attcgggcgt cgtcgccgac aaggccgccg cgatcggcgc gggcacgatg acgctgaccg
      721 tcggcggcgt cgccaagacg atcaccgtcg atgcgacgaa caacagcctc gacggcctcg
      781 ccaaggcgat caacgcgagc ggcgcgggcg tcaccgcgtc gatcatcgcc gacgaaggcg
      841 ggcaccgcct gatcctgaaa ggcccgaccg gcgaagcggg cgccttcact cttgccgccg
      901 acgccggcgc cgacccgggc ctttcggcct ttgcgacggg cggcggcatg accgagggcc
      961 aaagcgccgc caacgccgaa ttcacgatcg acggcgtcgc cttcagccgc gcgaccaaca
     1021 tcatcgacga cgtcgtcccc ggcatgtcgc tgacgctcaa aaaggcggct ccgggccagc
     1081 cagtcgacat cggcgcgagc cgcccgctcg acatgatcaa acagaccgtc ggcgatttcg
     1141 tcgccgtcta taaccagctc aagaaaagcc tcgtcgccgc atcgagcctg tcgggtccga
     1201 cgaccggatt gcgcgaactc gaacgcgaac tcggcgggct catccaccag gtggtttcga
     1261 gccacggcag catcaaccaa ctgtccgaca tcggtgtttc gataacgaag gaaggcctgc
     1321 tgtcggtcga caataaaaag ctcgacaagg cgctcgaaag tgacgccggc gcggtcgagg
     1381 cgctgttcaa cccgcgccgc gacgccaccc acaccgaaca gagcgacccc ggcatcgcct
     1441 tcgcgctcga cgcgatccgc gacaaggcgg tcggaaccaa tggcgcgatc gatcgcgtgt
     1501 cgaaatcgct cgacacgaaa aaggaaaatc tcgccgacca gctcgagaag atcgaggagc
     1561 gcgaggacgc gtaccggaag cgcctcgaga agcaatatgg ctcgctcgaa gcgaagctcg
     1621 ccgcgttcaa ggcgacgcaa tcctatctcg aacagcagat caagctctgg tcgaaccagg
     1681 gcaatgacta actagagcgt ggggaccagg accgtgaccg ctaccgcttc gaccgtccgg
     1741 gcgaccgggc tttatcgccg attgcagaac gaaagccgcg ctgccgccgc cgaccccgtc
     1801 gagctggtga cgatgcttta cgacgagctc gaaaccgccg tcggcgtcct cgcggcgatg
     1861 gtgcggcagg ggcagcgcat ttcggcgacc gagcccgcgc accgcgcgcg cgcgatcctg
     1921 atcggcctcg acgtcaatct cgaccgcgac aagggcggcg acgtcgcgac cgcgctgtcg
     1981 cgcgtctacc gcagcatgcg ccgcaagctc gacgatgccg tggcggcgaa cagcgccgag
     2041 gcgctgtccg aacttctcga cggaatcctg acgatcagcg ccgcctggcg ccagcttcgt
     2101 tagggtagca gcccgcgaca ggcgaacagc aaaaggcccg cccttcacat gaaggagcgg
     2161 gccggtgccg tgcatatttg gtagcccgct gggtacccgg ccgccctagg tggggacggg
     2221 cgaccgggtg gtgcgggagc ggaccagagc cccctttttt cagcgggtgc tgaaacttgt
     2281 gtacggcaga ccgtcaggcg gcctgctggt gaacgccata tttgcgcatc ttctcgatca
     2341 gcgtggtgcg cttcagcgtc agcaggcgcg cggcctccga aatgataccg tcggcaaggt
     2401 cgagcgcgac gtggatttgt tcgagctcga tcgtttcgat ctcgcgcttg aggtcgatcg
     2461 gacggcccgg cgccggcgtc ttcgccgtcg gcgcggccga agcgaagggc gccgcggcga
     2521 aatcgtccgc gctggcctgc accgcggcgg ggctgctggg gacagccagc cgcggcgcaa
     2581 gcgctgcggt ggggttcaaa agcatcgcga catcgtcggc gccgagcgcc tcgccgccat
     2641 ggagaacgct ggcgcgttcg acgaaattcc tgagttcgcg gacgttgccc ggccaggcgt
     2701 gctgcatgag gagcgccatc gcggcatcgt catagcggca tttggcgtcg gctggcatct
     2761 gacgctggaa gtggcggacg agcgccggaa tatcctcgac gcggctggcc aggctgggga
     2821 cctggagcac aaccacgccg agccggaaga acagatcttc gcggaacttg ccatcggcga
     2881 tcgcggcgcc gagatcctga tgggtggccg aaatgacgcg aacgtcgacg gctttcacct
     2941 cgctgctgcc gacgcggacg atcgtccggt cttcgagcac gcggagcagt ttgacctgca
     3001 tgtcgaaacg catgtcgccg atttcgtcga ggaagagcgt gccgccctcg ctcgcttcga
     3061 aatggccgat gcggcgggca tgggcgccgg tgaagctgcc cttttcatgc ccgaacaatt
     3121 cggattcgat gagttcgccg ggaatcgcgc cgcagttgat tgcggagaag gccttgcccg
     3181 cacggacgcc ttcgtcgtgg atcgcacggg cgaccagttc cttgcccgag cccgacgggc
     3241 cgcagagcat caccgaggcg ttcgagcgcg ccacgcggcg gatcatctcg cgcagccggc
     3301 aggttgccgg gctcgacccg atgatcagcg cttcgagcgc tgtattttca ttgtgcccca
     3361 gtgtcatttt ggtctccacc gcaccgcccg ctcggtgccc ccttcgatgg actcagcaat
     3421 tgacgaaact ggtaaacaaa agcttatcgc ctgttcacta agtccttcaa aacaaatgga
     3481 tattgccatt ttggaagtat tggcggcgca aacgcgttaa ctatctccga tatactgcca
     3541 aactggcact aattctcgcc cgccccccgc catcccagcg tgatcgagat gcggcgattg
     3601 cgcggatcga atcggtcgga cgggacatag ggctcgcgat cggcgacccc ctcgattcgc
     3661 gaaaatcgtt cggaagcgat gccgcggaac tcgagatagt ggcgcgtcac ctcggcgcgg
     3721 tcgaccgaca gacgccaatt gttggtgccc gacttcgccg accagggcgc ggcatcggtg
     3781 tgcccgcgga ccatcacccg gttgggcact tcggcgacca gcgccgcgac ctcgccgaac
     3841 agcttcgcgc ccgaaggggt cagctggctg gtgccgatcg cgaacatcga aacgtcggca
     3901 tcgtcgacga tgtcgatgcg cagcccttcc atcgtttcgg tgaagcgcag gttgcgcgcg
     3961 aggcgcttga ggtcggcccg gctctggagc cgctccttca gcgactgcgc gagcaggttg
     4021 aacttcttcg attcggcttc gcgctcgcgc cccgacgcct cgcgcggtcc gccgaccgcg
     4081 tcgcgcggga tcgtcatcgc gcgcgtgccc gtct
//
LOCUS       DATMSU010000392         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_158531,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000392 DATMSU010000000
VERSION     DATMSU010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158531"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VN034_10535"
     CDS             <1..484
                     /locus_tag="VN034_10535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV61082.1"
                     /translation="GAGWKGRAGSAIGCAPDTEALFREVVREGQRRGQLRIARLTTAG
                     GVAAMSSWFVAGKRGFGFKMTYDEALRSYAPGLLLMRHVAEQVGAEPDMFFDTCASPG
                     AHSGAAFWRDRRTMFDCAIAIGTPRRRRLFSGLMRAREAVRRRSGCLGDLPRQLTQSL
                     "
     gene            complement(446..3940)
                     /gene="mfd"
                     /locus_tag="VN034_10540"
     CDS             complement(446..3940)
                     /gene="mfd"
                     /locus_tag="VN034_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWV61083.1"
                     /translation="MTRPAADIFAAIGSAPAPLTLARTADGFLPLLLADLARASDKRL
                     VYVATDDAAMQAVADAAPFFAPDLIVHRFPAWDCLPYDRAGPSMRVSADRLATLSALQ
                     LPAKRGELILTTVAAITQRTLTPFRIRQLATTLASGQRIDRDALAELLVSNGFSRVDT
                     VADQGEFAVRGGILDLFPAGEETGLRVDFFGDEIESIRRFDPADQRSLGPAKALQLLP
                     ASETLLDEATIKRFRSSYRDLFGGQSTGDPLYQAVSDGRRQAGMDHWLPLFEERLATL
                     FDHIDPATPVLRGHRTEATAETRFAAIADYHANRVAAERESPGSYRPLAPETLYLTEA
                     EWAGAAKARPIHIVTPFDVPPSATVVDLESFAARDFTPERTADENVYAKVADHLSDER
                     RKGRRTIIASYSGGARERLSGLLRDHGVTSLAPADSWQDALGTASTDSGGATAMVVLP
                     LDHGFASDAISLLTEQDILGERLVRRQKRRKSADAFLAELATLSVGDLVVHLDHGIGR
                     YEGLTQIPVGSSPHDCVALTYAGGDKLYVPVENLDVLSRYGGESEGVGLDRLGGEAWQ
                     RRKARMKERIREIAGELLATAAQRALRSGELLAQDAAYPAFADRFPYQETDDQDRAIG
                     DVLADMASGRPMDRLVCGDVGFGKTEVALRAAFVAAMAGVQVAVVVPTTLLARQHYTN
                     FVERFKGFPIQIGRLSRLVPAAEAQKTRDGLASGQIDIVVGTHAVIAKSVEFKNLGLV
                     IVDEEQRFGVVHKERLKQLKTDVHVLTLTATPIPRTLQMAMSGLRELSVIQTPPVDRL
                     AVRTYVAPWDPVVIREALLREHDRGGQSFFVTPRIKDLPDIEEFLRTRVPEIKYVVAH
                     GQMAPGEVEERMSAFYDRKYDVLVSTTIVESGLDIPSANTLIVHRADRFGLAQLYQLR
                     GRVGRSKTRAYAYLTTLEGGAITDTAEKRLKLLGDLDTLGAGFQLASHDLDIRGAGNL
                     VGDEQSGHIREVGFELYQSMLEEAILVAKAEGMGAAPPREALSPVITVDAPILIPEDY
                     VPDLPLRMALYRRLNDASDRPALDAFAAEMIDRFGPLPPETANLIQLMEIKANAKAAG
                     IAKLDVGTKGALVSFHGDQFANVPGLIAYVERLKGRARIRPDNRLSVSGDWVSTGARL
                     NGALQLSKGLGKLARQIA"
BASE COUNT          598 a         1238 c         1456 g          649 t
ORIGIN      
        1 aggcgcgggg tggaaagggc gcgcggggag cgcgatcggt tgcgcgccgg acaccgaggc
       61 gctgttccgc gaggtggtgc gcgaagggca gcggcgcggc cagcttcgta tcgcgcggtt
      121 gacgaccgct ggcggcgtgg ccgcgatgtc gagctggttc gttgccggga aacgcgggtt
      181 cgggttcaag atgacctatg acgaggcgct gcgaagctat gcgccggggt tgttgctgat
      241 gcgccatgtc gccgagcagg tgggcgccga acccgacatg ttctttgaca cctgcgcatc
      301 gcccggtgcg catagcggtg cagccttctg gcgcgaccgc cgtacgatgt tcgactgcgc
      361 gatcgccatc ggcacgccgc gccgtcgccg cctgttttcc ggcctgatgc gcgcacgcga
      421 agcggtgcgg cgccgatcgg gctgtctagg cgatctgccg cgccagctta cccaatccct
      481 ttgagagttg cagcgccccg ttgagccgcg cgccggtgct gacccaatcg ccgctgaccg
      541 acagcctgtt gtcggggcgg atgcgcgcgc ggcccttgag ccgctcgaca taggcgatca
      601 gccccgggac gttcgcgaac tggtcgccgt ggaagctgac gagtgcgccc ttggtgccga
      661 cgtcgagctt ggcgatgccc gccgccttcg cattggcctt gatctccatc agctggatca
      721 ggttcgcggt ctcgggcggt agcggtccga aacggtcgat catctcggcg gcgaaggcgt
      781 cgagtgcggg gcggtcgctc gcgtcgttga ggcggcgata gagcgccatg cgcagcggca
      841 ggtcggggac gtaatcctcg gggatcagga tcggcgcgtc gacggtgatg acgggggaga
      901 gggcttcgcg cggcggtgcg gcgcccatgc cctcggcctt ggcgacgaga atcgcctcct
      961 caagcatcga ctgatagagt tcgaagccga cctcgcggat atgccccgac tgttcgtcgc
     1021 cgacgaggtt gcccgcgccg cgaatgtcga ggtcgtggct cgcgagctgg aagccggcgc
     1081 cgagggtatc gagatcgccg agcagcttga gccttttttc ggcggtgtcg gtgatcgcgc
     1141 cgccttcgag cgtggtgaga taggcataag cgcgggtctt cgaccgcccg acgcggccgc
     1201 gaagctggta gagctgcgcg aggccgaagc ggtcggcacg gtggacgatc agcgtgttcg
     1261 cgctcggaat gtcgagcccg ctctcgacga tcgtcgtcga gacgagcacg tcatatttgc
     1321 ggtcgtagaa ggcgctcatc cgctcttcga cctcgcccgg cgccatctgg ccgtgcgcga
     1381 cgacatattt gatctcgggc acccgcgtgc gcaggaactc ctcgatgtcg ggcaggtcct
     1441 tgatccgcgg cgtgacgaag aagctctgcc cgccgcgatc gtgctcgcgg agcaatgcct
     1501 cgcggatcac gacgggatcc catggcgcga cataggtgcg gaccgcgagg cggtcgaccg
     1561 gcggcgtctg gatgacgctg agttcgcgga ggcccgacat cgccatctgc agcgtgcgcg
     1621 ggatcggggt cgcggtgagg gtcaaaacgt ggacgtcggt cttgagctgc ttcagccgct
     1681 ccttgtggac gacgccgaag cgctgttcct catcgacgat gacgaggccg agattcttga
     1741 actctaccga tttcgcgatg acggcgtggg tgccgacgac gatatcgatc tggccgcttg
     1801 ccagcccgtc gcgcgtcttc tgcgcttcgg cggcgggaac gaggcgcgac agacgcccga
     1861 tctggatcgg gaagcctttg aaacgttcga caaaattggt gtagtgctgg cgcgcgagca
     1921 aagtcgtcgg gacgacgacc gcgacctgca cccccgccat cgccgcgacg aaggcggcgc
     1981 gtagcgcgac ctcggtcttg ccgaagccga cgtcgccgca gacgagccga tccatcgggc
     2041 gccccgacgc catgtcggcg agcacgtcgc cgatcgcccg gtcctgatcg tcggtttcct
     2101 gataggggaa gcggtcggca aacgccggat aggcggcatc ctgcgcgagc aattcgcccg
     2161 aacgcagcgc gcgctgggcc gcggtcgcga gcagttcgcc cgcgatctcg cggatgcgct
     2221 ccttcatccg cgccttgcgg cgttgccatg cttcgccgcc gagcctgtcg aggccgacgc
     2281 cctcgctctc gccgccatag cgcgagagga cgtcgagatt ttcgaccgga acgtagagct
     2341 tgtcgccgcc agcgtaagtg agcgcgacgc agtcgtgcgg gctgctgccg accgggatct
     2401 gggtcagccc ctcatagcgg ccgatgccgt gatcgaggtg gacgacgagg tcgccaacgc
     2461 tgagcgtcgc gagttcggcg aggaaggcgt cggcgctctt gcggcgcttc tgccgccgaa
     2521 cgaggcgctc gccgaggata tcctgttcgg tgagcaggct gatcgcgtcg gaggcgaagc
     2581 catggtcgag cgggaggacg accatcgcgg tcgcaccgcc gctgtccgtc gaggcggttc
     2641 cgagcgcgtc ctgccagctg tcggcgggcg cgagcgaggt gacgccatgg tcgcggagca
     2701 gccccgacag gcgctcgcgc gcgccgcccg aatagctcgc gatgatcgtg cggcggccct
     2761 tgcggcgctc gtcgctcaga tggtcggcga ccttcgcata gacattttcg tcggcggtcc
     2821 gctcgggagt gaaatcgcgc gcggcgaagc tttcgaggtc gacgacggtc gccgaggggg
     2881 gcacgtcgaa gggggtgacg atatggatcg ggcgcgcctt ggctgccccg gcccattcgg
     2941 cttcggtaag gtaaagcgtc tcgggcgcga gcgggcggta gctgcccggc gattcgcgct
     3001 cggcggcgac ccggttggca tgataatcgg cgatcgccgc gaagcgcgtc tcggcggtcg
     3061 cctcggtgcg gtggccgcgc agtactggtg tcgcgggatc gatatgatcg aacagcgtcg
     3121 ccagccgttc ctcgaacagc ggcagccaat ggtccatgcc cgcctgccgc cgcccgtcgc
     3181 tgaccgcctg atagagcgga tctcccgtcg actggccgcc gaacaggtcg cgataggatg
     3241 agcggaaacg cttgattgtc gcttcgtcga gcaaagtttc ggacgcgggg agaagctgta
     3301 aggccttggc ggggccgagg ctgcgctggt cggcggggtc gaaacgacga atgctttcga
     3361 tctcgtcgcc gaagaaatcg acgcgcagcc ccgtttcctc gcctgccgga aacaggtcga
     3421 ggatgccgcc gcgcaccgcg aattcgccct gatcggcgac ggtatcgacg cggctgaagc
     3481 cgttcgagac gagcagctcg gcgagcgcgt cgcgatcgat ccgctgcccc gacgcgaggg
     3541 tggtcgcgag ctggcggatg cgaaagggtg tgagcgtgcg ctgggtgatc gcggcgacgg
     3601 tggtgaggat cagctcgccg cgcttcgcgg gcagttgcag tgccgagagc gtcgccagcc
     3661 ggtcggcgct cacgcgcatc gacggtccgg cgcggtcata gggcaggcag tcccaagcgg
     3721 gaaagcggtg gacgatcaga tcgggcgcga agaagggcgc ggcgtcggcg accgcctgca
     3781 tcgccgcatc gtcggtcgcg acatagacga gacgcttgtc gctggcgcgc gcgagatcgg
     3841 cgagcagcag cggcaggaaa ccgtcggcgg tgcgcgccag cgtcagcggc gccggcgccg
     3901 atccgatcgc ggcgaaaatg tcggcggcgg ggcgggtcat g
//
LOCUS       DATMSU010000393         4653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_177388,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000393 DATMSU010000000
VERSION     DATMSU010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177388"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VN034_10545"
     CDS             complement(<1..1184)
                     /locus_tag="VN034_10545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWV61084.1"
                     /translation="MLQRTNMSSGNRAGSPTICVTGLRGFPHVMGGIESHCEELLPRI
                     KALWPGHRSVVLGRAPYLPEPVGEHRGVEIIGIPCPRSQHFEAIVSTFLAVLSARRRG
                     AGLIHIHAIGPGLLAPVARLLGLKVVVTHHGTDYHRAKWGPLARGALRAGEWLALNFA
                     DRVIAVSPSLAAQLRKSFPQRAQAVSYIPNGTSDLPGDADPALIFERLGIADGDFLLA
                     VARLVPEKGLHDLIDASERSDCTAKLVIAGSADHESEYARELLERASDRVIFAGVQSR
                     ATLKCLYEHCALFVMPSYHEGLPIAALEAASCGARMLLSDIPANLDIGLDRVNYFAVG
                     DVAELTARLNADCADFQVDRDTVRARFSWDRAAAETLEVYRAALAPRRPVPRPSTTPG
                     TAG"
     gene            complement(1222..2235)
                     /locus_tag="VN034_10550"
     CDS             complement(1222..2235)
                     /locus_tag="VN034_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWV61085.1"
                     /translation="MTDDSPILVTGAAGFIGHAVSERLLARGERVIGLDSFTDYYDVA
                     LKKARTARLAGNFNFRLIDGDIASPGLVSELVEANGIRRIVHLAAQAGVRYSLENPFA
                     YEHSNLKGHLAVLEAARHAGDIEHLVYASSSSVYGDRPIGGAGFREDEPCTAPVSLYA
                     ATKRSCELMSQSYATLYKLPLSGLRFFTVYGPWGRPDMAYYGFAQKILLGQPIEVFGE
                     GRMARDFTYIDDIVDGVVGVLDRSPAGGEHRLLNIGDCNPVGLMDMIAMLERALGRPA
                     VKLMRPMQPGDVTATYADISAINALTGYSPKVPLSRGIERFVDWYLSEIENGGRGPMA
                     AVA"
     gene            complement(2232..3581)
                     /locus_tag="VN034_10555"
     CDS             complement(2232..3581)
                     /locus_tag="VN034_10555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWV61086.1"
                     /translation="MRIVMIGSGYVGLVSGACFADFGHHVTCVDKDADKIARLRAGGI
                     PIYEPGLDGLVARNVAAGNLDFTTELCEAVGAADVVFIAVGTPSRRGDGHADLSFVHG
                     AAREIAGALSGFTLIVTKSTVPVGTGDEVERIIRECNPAAEFAVASNPEFLREGAAIE
                     DFKRPDRVVIGIEDERARAPMEEVYRPLSLNKAPIVFTRRRTSELIKYAANAFLAMKI
                     TYINEMADLCEQVGADVQEVARGIGLDNRIGGKFLNAGPGYGGSCFPKDTLALMKTAQ
                     DHDAPLRLIEATVAVNEQRKRAMGRKILAAIDGPVRGRTVAVLGLTFKPNTDDMRDAP
                     SIAIIQTLQDAGAKVRAYDPEGMDAARTVLSDVDYAASAYAAAQGAAATVIVTEWDAF
                     RALDLARLKAAMADPLLVDLRNIYRPAQAEAAGLRYVGIGRPGNPDEEEVSGERRAA"
     gene            3893..>4653
                     /locus_tag="VN034_10560"
     CDS             3893..>4653
                     /locus_tag="VN034_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV61087.1"
                     /translation="MLEAQRAAFFADLPVSLAIRRDRLARAMRMIEENADALCAALAA
                     DQTNQDAESAKHTEVVPALAVLRAARDGVGRWMRPEGGRGLLARFGGGDYTEYLPVGV
                     IGIAAPAALPLLQTADVLASALAAGNRVMLKFDAASPQLGDLIDRLAPGYFDPLELAI
                     ASDGAFANLAFDLLVTSGEQGDGVTIARSGKSPVIFGRSADFASAADKVIADKRVRGG
                     RVPLAPDYLLVPEEQEEAIAAWLWRAAMRPAANEAA"
BASE COUNT          791 a         1502 c         1597 g          763 t
ORIGIN      
        1 cccgccgtgc cgggggtggt cgaaggacgc gggaccggcc ggcgcggcgc aagcgcggcg
       61 cgatagactt cgagcgtctc cgccgccgcc ctgtcccagc tgaaacgcgc gcgcacggtg
      121 tcgcgatcaa cctggaagtc ggcgcaatcg gcgttgagcc gcgcggtgag ttcggcgacg
      181 tcgccgaccg cgaaataatt cacgcgatcg agcccgatgt cgagattggc cgggatgtcg
      241 ctgagcagca ttcgcgcgcc gcaactcgcc gcctcgagcg ccgcgatcgg caggccttcg
      301 tgataggagg gcatcacgaa caaggcgcaa tgttcgtaaa ggcatttcag cgtcgcgcgc
      361 gactggacgc ccgcgaagat cacgcggtcg ctcgcgcgtt cgaggagctc gcgcgcatat
      421 tcgctttcat gatcggccga gccggcgatc accagcttgg cggtgcagtc cgaccgctca
      481 gaggcgtcga tcaggtcgtg caggcccttt tctggaacga gccgcgcgac ggcgaggagg
      541 aagtcgccgt ccgcaatccc cagccgctcg aatatcagcg cgggatcggc atcgccgggc
      601 aggtcggagg tgccgttggg aatataggag accgcctgcg cgcgctgcgg aaaactcttg
      661 cgcaattgcg cggcgagcga gggggaaacg gcgatgacgc ggtcggcgaa gttgagcgcc
      721 agccactcgc cggcgcgcag cgcgccgcgc gcgagcgggc cccatttggc gcggtgataa
      781 tcggtgccgt ggtgcgtcac gacgaccttc aggccgagca gccgcgcgac cggcgccagc
      841 aatccggggc cgatcgcatg gatatggatc agcccggcgc cgcgcctccg cgccgacagc
      901 acggcgagga aggtcgagac gatcgcttcg aaatgctgcg agcgcgggca ggggatgccg
      961 atgatctcga ccccgcgatg ctcgcccacg ggctcgggca gatagggcgc gcggccgagc
     1021 acgaccgaac ggtggccggg ccacagcgcc ttgatccgcg gcagcagttc ctcgcaatga
     1081 ctctcgattc cgcccatgac atgcgggaag ccgcgcagac ccgtcacgca gatggtgggc
     1141 gaccccgccc gattgccgga agacatgttc gtccgttgca gcatcataaa actccaaaag
     1201 cgcccggccg gatttcccgg ctcaggccac cgctgccatc ggccctcttc caccattttc
     1261 tatttcgctg agataccagt cgacgaaccg ctcgatcccg cgggaaagcg gcaccttggg
     1321 gctgtatccg gtgagcgcgt tgatcgccga gatatcggca taggtcgcgg tcacgtcgcc
     1381 cggctgcatc ggccgcatca gcttgaccgc gggccggccg agcgcgcgtt cgagcatggc
     1441 gatcatgtcc atcagcccga ccggattgca gtcgccgatg ttgagcagcc ggtgctcgcc
     1501 gcccgccggc gacctgtcga gcacacccac cacgccatcg acgatgtcgt cgatataggt
     1561 gaagtcgcgc gccatccggc cctcgccgaa cacctcgatc ggctggccga gcaatatctt
     1621 ctgcgcgaag ccgtaatagg ccatgtcggg ccggccccac gggccataga cggtgaagaa
     1681 gcgcagcccg ctgagtggca gtttgtagag ggtggcatag ctctggctca tcaattcgca
     1741 gctgcgcttg gtcgcggcat aaagcgaaac cggcgcggtg cagggttcgt cctcgcggaa
     1801 acccgcgccg ccgatcgggc ggtcgccata gaccgagctc gacgaggcat agacgagatg
     1861 ctcgatatcg cccgcatggc gcgccgcctc gagcaccgcg agatggcctt tgaggttgct
     1921 atgctcatag gcgaaggggt tttcgaggct gtagcgcacg ccggcctggg ctgcgaggtg
     1981 gacgatgcgg cgaatgccgt tggcctcgac cagctcgctg accagcccgg ggctggcgat
     2041 gtcgccgtcg atcagccgga aattgaaatt gcccgccagc cgcgccgtgc gcgctttctt
     2101 cagcgcgacg tcataatagt cggtgaagct gtcgaggccg atcacgcgtt cgccgcgcgc
     2161 gaggagccgt tcggacaccg catggccgat gaaaccggcg gcgccggtga cgaggatggg
     2221 gctgtcgtcg gtcacgccgc gcgcctttcg cctgagactt cttcttcatc gggattgccg
     2281 ggccgcccga tgccgacata gcgcaggccc gccgcttcgg cctgtgcggg gcgatagatg
     2341 ttgcgcaggt cgaccagcag cggatcggcc atcgccgcct tgaggcgtgc gagatcgagc
     2401 gcgcggaacg cgtcccattc ggtgacgatc accgtcgccg ccgcgccttg cgccgcggca
     2461 taggcggagg ccgcatagtc cacatcgctc agcaccgtcc tggctgcgtc catgccctcg
     2521 ggatcatagg cgcgcacctt ggcaccggca tcctgcagcg tctggatgat cgcgatcgag
     2581 ggcgcgtccc gcatgtcgtc ggtattgggc ttgaaggtca gaccgagcac cgcgacggtg
     2641 cggccgcgga ccggcccgtc gatcgccgcg aggatcttgc ggcccatcgc gcgtttgcgc
     2701 tgctcgttga cggcgacggt cgcttcgatc agccgcagcg gcgcatcatg gtcctgcgcg
     2761 gtcttcatca gcgcgagcgt gtccttggga aagcaggagc cgccgtagcc cggtcccgcg
     2821 ttcaggaatt tgccgccgat ccggttgtcg agcccgatgc cgcgcgcgac ctcctgcaca
     2881 tcggcgccga cctgctcgca caggtccgcc atctcgttga tgtaggtgat cttcatcgcc
     2941 agaaaggcgt tcgccgcata tttgatcagc tcgctcgtgc gccgccgggt gaagacgatc
     3001 ggcgccttgt tcagcgacag cgggcgatag acctcttcca tcggcgcgcg ggcgcgctcg
     3061 tcctcgatgc cgatgacgac gcggtcgggg cgcttgaaat cctcgatcgc cgcgccttcg
     3121 cgcaggaatt cggggttgga ggcgacggcg aactcggccg cgggattgca ttcgcggatg
     3181 atccgttcga cctcgtcgcc ggtgccgacg ggaacggtcg acttggtgac gatcaaggtg
     3241 aagccgctca atgccccggc gatttcacgc gcggcgccat ggacgaagga gaggtcggcg
     3301 tggccgtcgc cgcgccggct cggcgtgccg accgcgatga acacgacgtc cgcggcgccg
     3361 accgcttcgc aaagctcggt ggtgaagtcc agattgcccg ccgcgacatt gcgcgcgacc
     3421 agaccgtcga ggccgggctc atagatggga atgccgccgg cgcgaagccg ggcgatcttg
     3481 tccgcatcct tgtcgacgca ggtcacgtga tggccgaaat cggcaaaaca cgcgcccgaa
     3541 acgagcccga catagccgga cccgatcatt acgatacgca ttacgcgttg ctcccttttt
     3601 atatgaaggc gattttgctg cgcttgcgaa caatcatcgc gccagcccct cgcacgtcaa
     3661 cttgccagct ttgaaaagag gcaattgaaa ggcgaagcga atgggccgca atcgcacgtc
     3721 aaaatgaaac aaaactgaaa aacggcgcgc gattataagc agataattcg cggcttcggc
     3781 taaacgaaga tcgggcggtt tggctgcaat gcgacgaaac gtttcgactt gaacttgcca
     3841 cgcgtcaccg ccggattaga agctggatgc gataagttgg gggaagggca ggatgctgga
     3901 agcgcaacgc gcggcatttt tcgccgacct gccggtgtcg ctggcgatcc gccgggaccg
     3961 gctggcgcgg gcgatgcgga tgatcgagga aaatgccgat gcgctgtgcg ccgcgctcgc
     4021 tgccgaccag acgaatcagg atgccgagtc ggcgaagcac accgaagtcg tgccggcgct
     4081 ggcggttctg cgcgcggcgc gggacggcgt cgggcgctgg atgcgccccg aaggcggccg
     4141 cgggctgctc gcgcggttcg gcggcggcga ttatacggaa tatctgccgg tgggggtgat
     4201 cggcatagcg gcgcccgccg cgctgccgct gctccagacg gcggatgtcc ttgccagtgc
     4261 gctcgcggcg ggcaatcggg tgatgctgaa attcgatgcc gcctcgccgc aactgggcga
     4321 cctgatcgac cggctcgcgc ccggctattt cgatccgctc gaactggcca tcgcatcgga
     4381 cggcgccttt gccaacctcg ccttcgactt gctcgtgacg agcggcgaac agggcgatgg
     4441 cgtgacgatc gcgcgttcgg gaaagtcgcc cgtcatcttc gggcgttcgg ccgatttcgc
     4501 cagcgccgcg gacaaggtga tcgcggacaa gcgcgtccgg ggcggacgcg tgccgctggc
     4561 gcccgattac ctgctcgttc ccgaggagca ggaagaggcg atcgccgcct ggctgtggcg
     4621 cgcggcgatg cggccggcgg cgaatgaagc ggc
//
LOCUS       DATMSU010000394         8762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_130310,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000394 DATMSU010000000
VERSION     DATMSU010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8762
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130310"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..525
                     /locus_tag="VN034_10565"
     CDS             115..525
                     /locus_tag="VN034_10565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HWV61088.1"
                     /translation="MEIRRYAASDFDGVKALWEEAFPNDPPWNRAEVAIPAKLAFQPE
                     LFFVALDGGAVIGSVMAGYDGHRGWLYSVAVGQACKRGGVGTALVREAERALHALGCE
                     KINLQVRAANEAVVQFYRALGYAVEDRISLGRRL"
     gene            complement(531..950)
                     /locus_tag="VN034_10570"
     CDS             complement(531..950)
                     /locus_tag="VN034_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV61089.1"
                     /translation="MERTASCHCGKLVLTCTGDPAKVSLCHCFDCQRRTGSLFSVAAF
                     FPRDGVALPAEHAKAFKRPSASGRDVSFHFCPDCGSSLWWEPERLPHLIGVAAGAFAD
                     RDFPMPEQAVWAEQRHAWLDLPLPEHLRNPVRTAPGD"
     gene            complement(1157..1987)
                     /locus_tag="VN034_10575"
     CDS             complement(1157..1987)
                     /locus_tag="VN034_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWV61090.1"
                     /translation="MWRFAGVDLVTARARIDAAFEAGVTLFDTADIYGFDTPGGFGSA
                     ETLLGEVFAEAPGLREAMFLATKGGIIPGVPYDSSAPYLASAIDASLRRLRTGRVELW
                     QIHRPDLLAHPQEIARTLEEAHAAGKIGAIGVSNFTPSQTAALARFLPVPLVSHQSEF
                     SPLHLAPLFDGLFDQSMEQGMAFLAWSPLGGGRLGDPADEHGRAVAALLDAKAAEYDV
                     SRAAATYSWVMAHPARPIPIVGTQKVERIREIPQAFIPRWTRAEWYAVLQVSMGERLP
                     "
     gene            complement(2063..2905)
                     /locus_tag="VN034_10580"
     CDS             complement(2063..2905)
                     /locus_tag="VN034_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV61091.1"
                     /translation="MRALLLSIALLTPSVPAFAQDAVVDTHVHLHKGEASLAEYRAQL
                     KAAGQSVDAFGAMWFGGPNQALAGNPSDISQRNDALIALAAKNPDMIAIATVHPYDGD
                     AALAEVDRVAARGVRLLKIHPHTQKFDAADPRVLTLVKHAGEKGMIVVMDNASIVPSD
                     NEKLFNLALAAPKTKFIFGHMGGLGFRFWNILALARTAENLFADNIYFDISASVILAA
                     DSPIEEEYVWTIRNVGVDHVLIASDFPQISLPKTLEAFAKLDLTDEERAKIRSGNARK
                     LLGL"
     gene            complement(3019..4650)
                     /locus_tag="VN034_10585"
     CDS             complement(3019..4650)
                     /locus_tag="VN034_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWV61092.1"
                     /translation="MKKKWLALCAILLAGAGGYWAYEANKYRLPGVLADWNDPVQPNR
                     AVAWQQGPAKAPDGKRPPNIILIVADDLGYNDISLNGGGVAGVVKTPNIDALAREGVN
                     FTTAYAANATCSPSRAAMMTGRYPTRFGFEYTAVPVEFAENLAHGEGVGPHRAIFHDE
                     LITPDIPAYPDMGVPASEVTIAEAVKAAGYHTLHIGKWHLGEAPKLQPQAQGFDESLA
                     ILAGAAMFLPEDDPGSVNAKLPWDPIDRFIWANLRHAVTFNGSQRFHPKGHMTDYFAD
                     EAVKAIEANKNRPFFLYLAFNAPHTPLQATKADYDKLPQIKDHKTRVYGAMIAQLDRR
                     IGDVMAKLKEAGIDDNTLVIFTSDNGGAWYNGMPDLNAPFRGWKATFFEGGIRTPLFM
                     RWPGGIAAGTQRADVTGHLDIFSTIAAAAGAKLPEGRTIDSENILAGPAKRPAMFWRS
                     GDYRAVRAGDWKLQVTKRPAKARLYNLAADPTERHDLAARDPQRVAELRALIEAQNKG
                     MAKPIWPGLVEGPVRIDVPLDAPWKDGQDYIYWTN"
     gene            4772..5395
                     /locus_tag="VN034_10590"
     CDS             4772..5395
                     /locus_tag="VN034_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61093.1"
                     /translation="MATGLKKMDAQDPARSRVDGRRERGRSSHRRIVEAMMELIVGGD
                     LSPSAARVAEEAGIGLRTVFRHFDDMDALYAEITATITEEVMPIVMAPYPDQPWRDNI
                     RELVGRRVRVFETTLPFRLAANIKRYQSPFLMAQYSRVVMLERELILRLLPAAVRADR
                     IGVEALCAALSFHSWRTLRHDQSLSVEEAGAVMQRMVEAQMAAIAAE"
     gene            complement(5399..7234)
                     /locus_tag="VN034_10595"
     CDS             complement(5399..7234)
                     /locus_tag="VN034_10595"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HWV61094.1"
                     /translation="MAVRWRTTRPGRIGASRCAAIIAIAALIGASPAHAQPARPAPDH
                     PNIVILLADDWGFSDVGAFGAEFATPNIDALAYAGMRFSNFHVAGSCSPSRAMLQTGV
                     MNHRNGLGNMPETIPDEHRGKPGYDTVMNHRVVTIAELLKAAGYRTYLTGKWHLGSDA
                     TRLPHARGYDRAFSLADAGADNFEQRPIEGMYDKANWTENGKPATLPKDYYSSRFVVQ
                     RMIDYIEADRKSGKPFFASINFLANHIPVQAPDSDIARYSAMYRDGWTALREARAKRA
                     AALGIMPAGMPMVTMPTTPDWKKLDADERVAAVRVMQAYAGMATAMDREVGRLVAHLK
                     ATGDYHNTIFVFLSDNGAEPTNPFASLRNRLFLGMQYDLAAANIGRRGSFSAIGPGWA
                     SAAASPLAGYKFTASEGGLRVPLAIAWPGHAEIRAGGISDGLAHVTDILPTLTELAGV
                     PGHGGRWRGRAVEPVTGRSLVPMLKGQPGSVHGDAPLGYELSGNAALFRGDYKLVRNL
                     APTGDGEWRLFNLKADPGETRDLAAAEPDRFKTMMADYRAYAKANGVLDMPAGYTADE
                     QINRYAFEHQGKPRLIRFGLWVGAFVLLVSGLVWGLRRRRRAKSG"
     gene            complement(7315..8103)
                     /locus_tag="VN034_10600"
     CDS             complement(7315..8103)
                     /locus_tag="VN034_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWV61095.1"
                     /translation="MSELLIERRGAVEIATLNRPERLNALNEGLVGDLNAYFGGLADR
                     PEVRVVVLRGAGRGFCAGLDIQEDRSSDETPVLRTLRTQTSIGNIYRKMRACPQPIVA
                     LGHGAACGGGLSLLLASDVRYAAPSFRCNAAYIRIGLGGCDMASSYFLPRLVGASLAS
                     EMILTGRFIDAERALRAGLVSEIVDEEALLDRGLALADEMLATSPHGLRLSKQALNLN
                     IDAPSLEAAMAIEDRQQVILSATDDHREALAAFLEKRAPDYRER"
     gene            complement(8100..>8762)
                     /locus_tag="VN034_10605"
     CDS             complement(8100..>8762)
                     /locus_tag="VN034_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61096.1"
                     /translation="VLAARRADKLAEQVAAIEAAGGQAIAVSMDVTDEASTIAAYDAA
                     EAAFGTVDTIVANAGVATEKMAMGLSVEEVDFLLAANVRGVFLTATEGAKRLDKAGSR
                     EKQNGRIVLIGSITAEKVFPATSVYGATKAAVRHLGKALAREWARRGISVNVIQPGYF
                     ESEMTAELFDSDVGAAMVKSFPRQRMRPPSDLHAPLLLLCSDAALGITGSVITVDDGQ
                     TL"
BASE COUNT         1450 a         2787 c         3015 g         1510 t
ORIGIN      
        1 gacgatgtgc gcgcaagcgg cggcgtcggg atcgcccgcg aacaacccgc gggtgcccga
       61 tgcggatgag atggcggcgc tctatgcgca ggtctgggcg ggttaggccc cgcgatggaa
      121 atccggcgct atgcggcatc cgacttcgac ggcgtaaaag cgctttggga agaggcgttt
      181 ccgaacgatc cgccttggaa ccgcgccgag gtcgcgatcc cggccaagct cgctttccag
      241 cccgagctgt ttttcgtggc gctcgacggc ggcgcggtga tcgggtcggt gatggcgggt
      301 tatgacgggc atcgcggctg gctttattcg gtcgcggtcg ggcaagcgtg caagcgcggc
      361 ggcgtcggca cggcgctcgt ccgcgaagcc gaacgggcgc tgcacgcgct cggatgcgaa
      421 aagatcaacc tgcaggtccg tgcggcgaac gaagcggtcg tccaattcta tcgcgcgctg
      481 ggatatgcgg tcgaggaccg catcagcctc ggccggcggc tttagggtga tcagtcgccg
      541 ggcgctgtcc tgaccggatt gcgcagatgc tcaggcagcg gcagatcgag ccaagcgtgg
      601 cgctgttcgg cccataccgc ctgttcgggc attggaaagt cgcgatcggc aaacgccccc
      661 gccgcgacgc cgatcaggtg cgggagccgt tcgggttccc accacagcga cgatccgcaa
      721 tcggggcaga aatgaaagga tacgtcgcgg ccgctggccg acggacgctt gaacgctttc
      781 gcgtgttcgg ctggcaatgc cacgccgtcg cggggaaaaa aggccgcgac gctgaacagc
      841 gatccggtgc gccgctggca atcgaagcaa tggcagagcg aaaccttcgc cggatcgccg
      901 gtgcaggtca ggacgagctt gccgcagtgg caggaggcgg tgcgttccat gaggcgaagg
      961 catatcaccg cgcggcaagc aaacaagatg cgccgatcac cctgccttgg ccgcttcgtt
     1021 cattgacccg taaggcgcac cctgcttaag cgcgctcgcc ccgcctcccg ctctcctacg
     1081 tctcgatgtg gaggcggggc atccctttca aaaatctcgt tattgcgggt ccggggcaaa
     1141 ccgccgcgcg tagcgcttat ggcagccgct ctcccatcga cacttgcagc accgcatacc
     1201 attcggcgcg cgtccagcgc gggatgaacg cctgcgggat ttccctgatc cgttcgacct
     1261 tctgcgtgcc gacgatcggg atcggacgcg cggggtgcgc catcacccag ctataggtcg
     1321 ccgcggcgcg cgagacgtca tattcggcgg ctttcgcgtc gagcagcgcg gcgaccgccc
     1381 ggccgtgctc gtcggctgga tcgccaaggc ggccaccgcc gagcggcgac caggcgagga
     1441 aggccatgcc ctgctccatc gactgatcga aaaggccgtc gaaaagcggg gcgaggtgga
     1501 gcggtgaaaa ttcgctctga tggctgacca gcggaacggg aaggaagcgg gcaagcgccg
     1561 ccgtctggga cggcgtgaag ttggagacgc cgatcgcgcc gatctttccc gcagcatgcg
     1621 cttcttcgag cgtgcgggcg atctcctgcg ggtgggcgag cagatcgggg cggtgaatct
     1681 gccacagctc gacccggccg gtacgcaggc ggcggagcga agcgtcgatc gccgacgcca
     1741 gatagggtgc gctgctgtca taaggcacgc cggggatgat cccgcccttg gtcgcgagga
     1801 acatcgcctc gcgaagcccg ggggcttcgg cgaagacctc accgagcagg gtttcggccg
     1861 agccgaaacc gccgggcgtg tcgaagccat agatgtcggc ggtgtcgaac agcgttacgc
     1921 ccgcctcgaa cgccgcgtcg atgcgcgcgc gtgcggtgac aaggtcgacg ccggcaaagc
     1981 gccacatgcc ccaggcgatc ggcgcaaccg cgtgcccggc gatgtccaca ggagcgggag
     2041 cgggaagatg aaccggcacc ggtcagagcc cgagcagctt gcgcgcgttg cccgaacgga
     2101 tcttcgcgcg ctcttcgtcg gtgaggtcga gtttggcgaa ggcctcgagc gtcttgggca
     2161 gcgaaatctg cgggaaatcg gacgcgatca gcacatggtc gaccccgacg ttgcggatcg
     2221 tccagacata ttcctcctcg atcggcgagt ccgccgcgag gatcacgctc gccgaaatgt
     2281 cgaaatagat attgtcggcg aagagatttt ccgcggtccg cgcgagcgcg aggatattcc
     2341 agaagcggaa gccgagcccg cccatatggc cgaaaatgaa cttggtcttc ggtgccgcga
     2401 gcgcgaggtt gaagagcttc tcattgtcgc tggggacgat gctggcattg tccatgacga
     2461 cgatcatgcc tttctcgccc gcatgcttga cgagcgtcag cacgcgcggg tcggcggcgt
     2521 cgaatttctg cgtgtggggg tggatcttga gcaggcggac gccgcgcgcg gcgacgcggt
     2581 cgacttcggc gagcgcggca tcgccgtcat agggatggac cgtcgcgatc gctatcatgt
     2641 cggggttctt cgcggcgagc gcgatcagcg catcgttgcg ctgggatatg tcggagggat
     2701 tgcccgcgag cgcctgattc ggaccgccga accacatcgc gccgaaggcg tcgaccgatt
     2761 ggcccgcggc tttcagttgc gcccgatatt cggccagcga cgcctcgcct ttgtggaggt
     2821 gaacatgggt atcgacgacg gcgtcctgcg caaatgcagg caccgacggc gtaagcaagg
     2881 cgatcgaaag cagcagggct cgcatgatgt ctccagtgcg cgaggggagg aattccccga
     2941 cctgccacaa aggatgcgcg ccgccaatta cctcgcgacc accgcgatca gcttttccga
     3001 tgctggaacg gccccgcctc agttggtcca gtaaatatag tcctgtccgt ccttccacgg
     3061 cgcgtcgagc ggcacgtcga tgcgcaccgg cccttcgaca aggccgggcc agatcggttt
     3121 cgccatgcct ttgttctgcg cctcgatcag cgcgcgcagc tcggcgacgc gttgcgggtc
     3181 gcgcgcggcg agatcgtggc gctcggtcgg gtcggcggcg agattgtaga ggcgcgcctt
     3241 tgcggggcgc ttcgtcacct gcaatttcca gtcgcccgcg cgcaccgcgc gataatcgcc
     3301 cgagcgccag aacatcgcgg ggcgcttcgc ggggccggcg aggatatttt cgctgtcgat
     3361 cgttcggccc tccggcagct tcgcgccggc cgcggcggcg atcgtcgaga agatgtcgag
     3421 atgcccggtg acgtcggcgc gctgcgtccc cgccgcgatt ccgcccggcc agcgcatgaa
     3481 cagcggcgtg cggataccgc cttcgaagaa ggtcgccttc cagccgcgga agggcgcgtt
     3541 cagatcgggc atgccgttat accaggcacc gccattgtcg ctggtgaaga tgacgagcgt
     3601 attgtcgtcg atccccgcct ctttcagctt tgccatcacg tcgccgatgc gccggtcgag
     3661 ctgcgcgatc atcgcgccgt aaacgcgcgt tttgtgatcc ttgatctgcg ggagtttgtc
     3721 gtagtcagcc ttggtcgcct gtagcggcgt gtgcggcgcg ttgaacgcga gatacaggaa
     3781 gaaaggccgg ttcttgttgg cttcgatcgc cttgaccgcc tcgtcggcga aatagtcggt
     3841 catatgcccc ttgggatgga aacgctggct gccgttgaag gtgaccgcgt ggcggaggtt
     3901 ggcccagatg aagcggtcga tcgggtccca cggcagcttg gcgttgacgc tgcccggatc
     3961 atcctccggc aagaacatcg cggcccccgc gaggatcgcg aggctttcgt cgaagccctg
     4021 ggcctgcggc tggagcttcg gtgcttcgcc gagatgccat ttgccgatgt ggagcgtgtg
     4081 atagcccgcc gccttcaccg cctcggcgat cgtcacctcg ctcgcgggga cgcccatgtc
     4141 gggataggcg ggaatgtcgg gggtgatcag ttcgtcgtgg aagatcgcgc ggtgcggtcc
     4201 gacgccttcg ccatgcgcga gattttcggc gaactcgacg gggaccgccg tatattcgaa
     4261 gccgaagcgc gtcggatagc gccccgtcat catcgcggcg cgcgacggcg agcaggtcgc
     4321 gttcgcggca taggcggtgg tgaaattgac gccctcgcgc gcgagcgcgt cgatgttggg
     4381 cgtcttgacg acccccgcca cgccgccgcc gttgaggctg atgtcgttat agccgagatc
     4441 atcggcgacg atcaggatga tgttcggcgg gcgcttgccg tcgggagcct tggccggccc
     4501 ctgctgccag gcgaccgcgc ggttcggctg tacgggatcg ttccagtccg caaggacgcc
     4561 gggaagccgg tatttgttcg cctcatatgc ccaatatccg cctgcgcccg cgagtagaat
     4621 tgcgcagagg gcgagccatt ttttcttcat gatcttcccc catatcgcgg cgacggcttg
     4681 ctgcgaaact attgacacta tatgtgtcac tatattggcc ggcacgcaag atcgtttgac
     4741 cccggcgcca agtcgggcca gcagggacgc gatggcgaca gggctgaaga agatggatgc
     4801 gcaagacccc gcacgctcgc gggtcgacgg ccgccgcgag cgcgggcggt cgagccaccg
     4861 gcgcatcgtc gaggcgatga tggagctgat cgtggggggc gacctgtcgc cgagcgcggc
     4921 gcgcgtcgcc gaggaggcgg gcattgggct gcgcaccgtg ttccgccatt tcgacgatat
     4981 ggacgcgctt tatgccgaga ttaccgcgac gatcaccgaa gaggtgatgc cgatcgtgat
     5041 ggcgccctat cccgaccagc cgtggcggga caatatccgc gaactcgtcg gccggcgcgt
     5101 gcgggtgttc gaaacgacgc tgccctttcg tctcgccgcc aatatcaagc ggtatcagtc
     5161 gcccttcctg atggcgcaat atagccgcgt cgtgatgctc gagcgcgagt tgatcctgcg
     5221 cctgctgccg gctgccgtgc gcgccgaccg gatcggcgtc gaagcactgt gcgccgcact
     5281 atccttccat agctggcgca cgctgcgcca cgatcagagc ctgtcggtcg aggaggcggg
     5341 cgcggtgatg cagcggatgg tcgaagcaca aatggccgcg atcgccgccg aataatggct
     5401 atccgctttt cgcgcggcgc cggcggcgga ggccccagac cagccccgac accagcagga
     5461 cgaaggcgcc gacccacagg ccgaagcgga tcaggcgggg cttgccctga tgttcgaagg
     5521 catagcggtt gatctgttcg tccgcggtat agcccgcggg catgtcgagc acgccgttcg
     5581 ccttcgcata ggcgcgatag tcggccatca tcgtcttgaa gcggtcgggc tcggcggcgg
     5641 cgaggtcgcg cgtctcgccg ggatcggcct tgaggttgaa gagccgccac tcgccgtcgc
     5701 cggtcggcgc gaggttgcgc acgagtttgt agtcgccgcg gaacagcgcc gcattgcccg
     5761 acagctcgta gccgagcggc gcgtcgccgt ggacgcttcc cggctgtcct ttcagcatcg
     5821 gaacgaggct gcgacccgtc acgggctcga ccgccctgcc gcgccagcgt ccgccatggc
     5881 ccggcacgcc agccagctcg gtcagcgtcg gcaagatgtc ggtgacatgc gcgaggccgt
     5941 cgctgatgcc gccggcgcgg atttcagcat ggcccggcca ggcgatggcc agcgggacgc
     6001 gcagaccgcc ttcggacgcg gtgaatttat agccagcgag cggcgacgcc gccgcgctcg
     6061 cccagccggg accgatcgcc gagaaactgc cgcgccgtcc gatgttcgcg gccgcgagat
     6121 catattgcat gccgaggaac agccgattgc gcagactggc gaagggattg gtcggctcgg
     6181 cgccattgtc cgacaggaag acgaagatcg tattgtggta atcgccggtc gccttcagat
     6241 gtgcgacgag gcggccgact tcacggtcca tcgcggtcgc catgccggca taggcctgca
     6301 tcacgcgcac cgccgccacg cgttcgtcgg cgtcgagctt cttccagtcg ggcgtcgtcg
     6361 gcatcgtcac catcggcatg cctgcaggca tgatgccgag cgccgccgca cgcttcgcac
     6421 gcgcttcgcg cagcgcggtc cagccgtccc tgtacatcgc cgaatagcgt gcgatatcgc
     6481 tgtcgggcgc ctgcaccggg atgtggttgg cgaggaagtt gatcgaagcg aagaagggtt
     6541 tgccgctctt ccggtccgcc tcgatataat cgatcatccg ctgcacgacg aagcgcgacg
     6601 aataatagtc cttgggcagg gtcgcgggct tgccgttctc ggtccagttc gccttgtcgt
     6661 acatgccctc gatcgggcgc tgttcgaaat tgtcggcgcc ggcatcggca aggctgaacg
     6721 cgcggtcgta gccgcgcgcg tgcggcaacc gcgtcgcgtc gctgccgaga tgccattttc
     6781 cggtgagata ggtgcggtaa cccgccgcct tcaaaagctc ggcgatcgtc acgacgcggt
     6841 ggttcatcac cgtgtcgtaa ccgggcttgc cgcgatgttc gtcggggatc gtctcgggca
     6901 tgttgccgag gccgttccga tggttcatca cccccgtctg cagcatcgcg cgcgacggcg
     6961 agcaggaccc cgcgacatgg aaattggaaa aacgcatccc ggcataagcg agcgcgtcga
     7021 tgttcggggt cgcgaattcg gcgccgaacg cgccgacgtc tgaaaagccc cagtcgtcgg
     7081 caagcaggat gacgatgttc ggatggtccg gcgcaggcct ggcgggttgc gcgtgggcgg
     7141 gagaagcgcc gatcagcgcg gcgattgcga tgatggcagc acaccgggac gcgcctatgc
     7201 gacccggcct agtggtcctc cagcgtactg ccatcactcc accccctcgg gatgtgacac
     7261 ttactatgcc aatatatatt caagtcaatg ggggcgatgc gggatcgatc aaccttatcg
     7321 ttcgcgatag tcgggcgcgc gtttttcgag gaaggcggcg agcgcctcgc gatgatcgtc
     7381 ggtcgccgag aggatcacct gctgccgatc ctcgatcgcc atcgccgcct cgaggcttgg
     7441 cgcgtcgatg ttgaggttca gcgcctgttt cgaaaggcga aggccatggg gcgaggtcgc
     7501 gagcatctca tcggcgagcg cgagaccgcg gtccagcagc gcctcttcgt cgacgatctc
     7561 gctgacgagg cccgcacgca gtgcgcgttc ggcgtcgatg aaacggccgg tcaggatcat
     7621 ctcggaagcg aggctcgcgc cgacgaggcg cggcagaaaa taactcgacg ccatgtcgca
     7681 gccgccgagg ccgatgcgga tataggcagc gttgcagcgg aacgacggcg ccgcatagcg
     7741 cacgtcggag gcaagcagca gcgacagccc gccgccgcac gccgcaccgt ggccgagcgc
     7801 gacgatcggc tgcggacagg cgcgcatctt gcgatagata ttgccgatgc tggtctgggt
     7861 gcgcagcgtg cgcagcaccg gggtctcgtc cgaggagcga tcttcctgaa tgtcgagccc
     7921 ggcgcagaag ccgcgcccgg cgccgcgcag gaccacgacg cgcacttcgg ggcgatcggc
     7981 gaggccgccg aaataggcat tgagatcgcc gaccagccct tcgttcagcg cgttgaggcg
     8041 ctcgggccgg ttgagcgtcg cgatctcgac cgcgccgcgg cgttcgatca gcagttcgct
     8101 cacaaggtct gcccgtcgtc gacggtgatc acgctgccgg tgatgccgag cgcggcgtcg
     8161 gagcagagca ggagcagcgg cgcatgaaga tcgctcggcg gccgcatccg ctggcgggga
     8221 aagctcttca ccatcgccgc gccgacatcg ctgtcgaaca gttcggcggt catttcggat
     8281 tcgaaatagc cgggctggat cacattgacg ctgatcccgc gccgcgccca ttcgcgggcg
     8341 agcgccttgc ccagatgacg caccgccgcc ttggtcgcgc catagaccga ggtcgcggga
     8401 aagaccttct cggcggtgat cgatccgatc aggacgatgc gaccgttctg cttctcgcgg
     8461 ctgcccgcct tgtcgagccg cttcgcgcct tcggtcgcgg tcaggaagac gccgcgcaca
     8521 ttggctgcaa gcaggaagtc gacctcctcc accgacagcc ccatcgccat cttctcggtc
     8581 gcgacgccgg cgttggcgac gatcgtatcg acggtgccga aagcggcctc ggcggcgtca
     8641 taggcggcga tggtcgacgc ctcgtcggtg acgtccatgc tgaccgcgat cgcctgcccg
     8701 cccgccgctt cgatcgcggc gacctgctcg gcaagcttgt cggcgcggcg cgcggcgagc
     8761 ac
//
LOCUS       DATMSU010000395         5864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_64345, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000395 DATMSU010000000
VERSION     DATMSU010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64345"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VN034_10610"
     CDS             <1..689
                     /locus_tag="VN034_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61097.1"
                     /translation="ALLLASEGAKVAVTDLDLAACEAVAAEAGPNARAFALDVADGEA
                     IQRTVAEIAENFGAIDILINNAGVSSFAPLDAGDEYEAIWHRAIAVMLTAHQRMVRAA
                     LPWLRQSDAARIVNIASTEGLGATPGDTPYVAAKSGVVGLTRGLAVDLGKDGITVNCI
                     CPGPIRTGMTDKVPEEDKLLFARRHTALRRYGEPEEVAHVTLSLVLPAASYITGAVIP
                     VDGGLMARNA"
     gene            complement(744..1154)
                     /locus_tag="VN034_10615"
     CDS             complement(744..1154)
                     /locus_tag="VN034_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HWV61098.1"
                     /translation="MRSLDDIFEEYDFLDGDDRYRLLIELGRTLEPMPDALKTDATLV
                     RGCSASVWVYPVPRDDGRLHFLADSNAAITKGIVALVLSAVQDKPASEVAAMDVAAAL
                     APFDLTRQLSSNRTQGVPNMIALVQDTARRIAAD"
     gene            complement(1286..1531)
                     /locus_tag="VN034_10620"
     CDS             complement(1286..1531)
                     /locus_tag="VN034_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61099.1"
                     /translation="MAFITPDFTAAAVALSALSIVSLVALRGWRDWIQLKREELAAAH
                     VAAPNDPGVPNAGSRIEIADLKERLRKLEAIAAGVDL"
     gene            complement(1555..1818)
                     /locus_tag="VN034_10625"
     CDS             complement(1555..1818)
                     /locus_tag="VN034_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61100.1"
                     /translation="MGPFEMVIGIVLIVTVGSIIRAKHGIRRDHKGGEYYVAQDNSET
                     KALQAEIRSLKERIQVLERIATDNNRAVTLDQEIEKLRDSSKI"
     gene            complement(1854..2117)
                     /locus_tag="VN034_10630"
     CDS             complement(1854..2117)
                     /locus_tag="VN034_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61101.1"
                     /translation="MEAIVILLIVVGLPVTLGIGYAAYERHLKFKERQFKAITNETAE
                     KAAQYAAHTERLEARVRVLERIVTDKGIDVADEIEKLRDAPLN"
     gene            complement(2129..2449)
                     /locus_tag="VN034_10635"
     CDS             complement(2129..2449)
                     /locus_tag="VN034_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61102.1"
                     /translation="MDFILFKEAIAPVAVIIALAAIGGWIFTTWLRIKHGYPLENSWG
                     KAIYPRTSDEAMERVKLISQENAQLRAELGSVKDRLAVIERIVTDESHRVASEIEALR
                     RPAN"
     gene            complement(2461..2760)
                     /locus_tag="VN034_10640"
     CDS             complement(2461..2760)
                     /locus_tag="VN034_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61103.1"
                     /translation="MNFGGTTFVLAIIALSIGGWMFTTWIRAKHGYPVENEWGGTVHR
                     TDPDADRKIALLTDDNAKLAGQVSRLEERISVLERIATEEGGKAAQLADQIDRLR"
     gene            complement(2803..3183)
                     /gene="pspC"
                     /locus_tag="VN034_10645"
     CDS             complement(2803..3183)
                     /gene="pspC"
                     /locus_tag="VN034_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HWV61104.1"
                     /translation="MSASRTKFYLDKQNAKWSGVCAGIADYSGIDVLWVRVGAVLLTL
                     MGGFPWTLIAYWMVAWMGTAKPFALYGSNEEAKFWQGVRSNPSASTRDIRSRFRDIDR
                     RLADIEQYYTSHNRRLADEIDALR"
     gene            complement(3185..3463)
                     /gene="pspB"
                     /locus_tag="VN034_10650"
     CDS             complement(3185..3463)
                     /gene="pspB"
                     /locus_tag="VN034_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HWV61105.1"
                     /translation="MEEVIIVPIVIGTLFLGLPWLILHYITKWKQAKTLTGEDEQLLD
                     ELYDTARRLENRLHTVERIISADHPDFRPAVRSDEDLAQLENMTNRRN"
     gene            complement(3476..4138)
                     /gene="pspA"
                     /locus_tag="VN034_10655"
     CDS             complement(3476..4138)
                     /gene="pspA"
                     /locus_tag="VN034_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HWV61106.1"
                     /translation="MGIFSRTRDIIAANVTDLLDRAEDPEKMIRQIIFEMNETLVEVR
                     ASAARTIADQKEMRRHIAKLESLQDSWKEKAELALSKDREDLATAALVEKQKAGDMAE
                     KLKAEIAVLDDALTGYEADIAKLQKKLSEARARQSSVVNRLESAENRYKLREMTNGDR
                     VEDAFSRFEILERRVDEAEGRADALGLGYKKSLDEEIAELQSADKVADELAALKAAQG
                     KN"
     gene            complement(4240..4410)
                     /locus_tag="VN034_10660"
     CDS             complement(4240..4410)
                     /locus_tag="VN034_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61107.1"
                     /translation="MKTMFAQAATFALSAAGALAILVGVIDQPQVAASVTGTPAGPLV
                     AAVMSPAVSALA"
     gene            4588..5613
                     /gene="pspF"
                     /locus_tag="VN034_10665"
     CDS             4588..5613
                     /gene="pspF"
                     /locus_tag="VN034_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HWV61108.1"
                     /translation="MERETQFIGQSAAFQDAVERASLAASLDRPVLVIGERGTGKELI
                     AERLHRLSTRWDRPYVIMNCAAMPETLIESELFGHEAGAFTGATRSRAGRFEEADGGT
                     LFLDELATMSMGAQERLLRAVEYGEVTRVGASRPIRVDVRIVAATNEHLPALVDDNRF
                     RADLLDRLSFEVITLPPLRAREGDIEVLAEYFGTRMAAVIGWDEWPGFGPRALAAMEG
                     HDWPGNVRELRNVIERAIYRWADPTKPVDALSFDPFASPWQPAVRKAAAKAETPAAPN
                     ADPAPATSTPSGPVSDLRAAVDGYEKTILADTMARCRFNQKVAAEALGLSYDQIRHAL
                     KKHGLNQ"
BASE COUNT          948 a         1848 c         1958 g         1110 t
ORIGIN      
        1 ccgcgctgct tcttgccagc gagggcgcga aggtcgcggt caccgacctc gatctcgccg
       61 cctgcgaagc cgtcgcggcc gaggccggcc cgaacgcccg ggctttcgcg ctcgacgttg
      121 ccgacggcga ggcgatccag cgcaccgtcg ccgagatcgc cgaaaacttc ggcgccattg
      181 acatcctgat caacaacgcc ggcgtctcga gcttcgcccc gctcgacgcc ggggacgaat
      241 atgaagcgat ctggcaccgc gcgatcgcgg tgatgctgac cgcacaccag cgcatggtcc
      301 gcgccgcatt gccctggctg cgccagagcg acgccgcgcg catcgtcaac atcgcctcga
      361 ccgaagggct aggcgcgacg ccgggcgaca cgccttatgt ggccgccaag tcgggcgtgg
      421 ttggcctgac acgcggcctc gccgtcgatc tcggcaagga cggcatcacg gtcaactgca
      481 tctgccccgg cccgatccgc accggcatga ccgacaaggt gcccgaggag gacaagctgc
      541 tcttcgcgcg gcgccacacc gcgctccgcc gctatggcga acccgaggaa gtcgcgcatg
      601 tgacgctgag cctcgtgctg ccggcggcga gctacatcac cggcgcggtg atcccggtcg
      661 acggcgggtt gatggcgcgc aacgcgtgaa gggcggcatc gggcgggaaa gccggtctcg
      721 ccgggcgacg acggactgcg cggtcagtcc gcggcgatgc ggcgggcggt gtcctggacg
      781 agcgcgatca tattggggac gccctgcgtg cggttcgacg acagctggcg cgtgaggtcg
      841 aagggggcga gcgccgccgc gacgtccatc gccgcgacct cgctcgccgg tttgtcctgc
      901 accgccgaaa gcacgagcgc gacgatcccc ttggtgatcg cggcattgct gtcggcgagg
      961 aaatggagcc ggccgtcgtc ccgcggcacc ggatagaccc agacgctcgc cgagcagccg
     1021 cgcaccagcg tcgcgtcggt cttgagcgca tcgggcatcg gctctagcgt gcggccgagc
     1081 tcgatcagca gacgatagcg atcgtcgccg tcgagaaaat catattcttc gaagatgtcg
     1141 tcgagactgc gcatggcggc gcccctcgca cagatgccgc caagttggaa ggccctgaac
     1201 gacgtcggcc cgctcggctg ccggtcgccg gacggagggc gatagacagc ggagcgggcc
     1261 ggtgccgcgt tggacgaagg gacggttaga ggtcgacccc cgccgcgatc gcttccagct
     1321 tgcgcagccg ttccttgagg tcggcgatct cgatccgcga cccggcattg ggaacgcccg
     1381 gatcgttcgg cgccgcgacg tgcgccgccg ccagttcctc gcgcttgagc tggatccagt
     1441 cgcgccagcc gcgcagcgca acgaggctga cgatgctcag cgcgctgagc gcgaccgctg
     1501 cggcggtgaa gtcgggagtg atgaaagcca tgagcgttgc tcctttgccg gcattcagat
     1561 tttgctgctg tcgcgcagct tctcgatttc ctggtcgagc gtgacagcgc ggttattgtc
     1621 ggtggcgatg cgttcgagca cctggatgcg ctccttgagc gaacggatct cggcctgcag
     1681 cgctttggtc tcgctattgt cctgggcgac ataatattcg ccccccttgt gatcgcggcg
     1741 gataccgtgc ttggcgcgga tgatgctgcc gaccgtcacg atgaggacga tgccgatgac
     1801 catttcaaac gggcccatgg ggaggttcct ttcctaattc tcttatcgtt gcgtcagttg
     1861 agcggggcgt cgcgcagctt ttcgatctcg tcggcgacat caattccctt gtcggtaacg
     1921 atgcgctcga gaacgcggac gcgtgcctcc agccgttcgg tgtgcgccgc atattgggcg
     1981 gccttttcgg cggtctcgtt ggtgatcgcc ttgaactgcc gctccttgaa cttgaggtgg
     2041 cgttcatagg cggcgtagcc gatgccgagc gtgacgggca gcccgacgac gatcaggagg
     2101 atgacgatgg cttccatgct tgctctcctc agttcgccgg gcgccgcagc gcctcgatct
     2161 cgcttgccac ccggtgactt tcgtcggtga cgatgcgctc gatcaccgcg aggcgatcct
     2221 ttaccgaacc gagttcggcg cgcagctggg cattttcctg gctgatcagc ttgacgcgct
     2281 ccatcgcctc gtcgctggtc ctgggataga tcgccttgcc ccagctgttt tcgagcggat
     2341 agccatgctt gatgcggagc caggtcgtga agatccagcc gccgatcgcg gcaagggcga
     2401 taatgaccgc tacgggggca atcgcttcct tgaacagaat gaagtccatt atctttcctc
     2461 tcagcgcagg cggtcgatct ggtcggcgag ctgcgccgcc ttcccgccct cttcggtcgc
     2521 gatgcgttcg agcaccgaaa tccgttcttc cagccggctg acctgccccg ccagcttggc
     2581 gttgtcgtcg gtcagcaggg cgatttttcg atcagcgtcg gggtcggttc gatgaaccgt
     2641 gccaccccat tcattctcga cgggatagcc gtgcttggcg cggatccagg tggtgaacat
     2701 ccagccgccg atcgacagtg cgatgatggc gaggacgaaa gtggtaccac cgaaattcat
     2761 aattagtctc cctgtttgcc ccagttcgat cgatgttccg gattaccgga gagcgtcgat
     2821 ctcgtcggca aggcggcgat tatgactggt gtaatattgt tcgatgtcgg cgagccggcg
     2881 gtcgatgtcg cggaagcgcg agcggatgtc gcgggtcgac gcgctggggt tcgagcgcac
     2941 gccctgccag aacttcgctt cctcgttcga gccatagagc gcgaagggtt tcgccgtgcc
     3001 catccaggcg accatccaat aggcgatcag cgtccagggg aagccgccca tcagcgtgag
     3061 cagcaccgcg ccgacgcgga cccagagcac gtcgatcccg ctatagtccg cgatccccgc
     3121 gcacacgccg ctccatttgg cgttctgctt gtcgagatag aatttggtgc ggctggcaga
     3181 catctcagtt cctccggttc gtcatgtttt cgagttgcgc caggtcttca tcgctgcgta
     3241 ccgcggggcg gaaatcggga tggtcggcgc tgatgatgcg ttcgacggta tgtagacgat
     3301 tctcgagccg acgagccgta tcgtaaagtt cgtcgagcag ctgttcatcc tctccggtca
     3361 gcgtcttggc ctgcttccac ttggtgatgt agtggaggat cagccagggc agaccgagga
     3421 agagcgttcc gatgacgatc gggacaatga tgacttcttc catcggtccg gctccttaat
     3481 tcttgccctg cgcggccttc agcgcggcga gttcgtcggc gaccttgtcg gcggactgca
     3541 gctcggcgat ttcctcgtcg agcgacttct tgtagccgag gcccagcgca tcggcacggc
     3601 cttcggcctc gtcgacgcgg cgttcgagga tttcgaagcg cgagaaggcg tcttcgacgc
     3661 ggtcaccgtt ggtcatctcg cgcagcttgt agcgattctc ggcgctttca aggcggttga
     3721 ccacgctcga ctggcgcgcg cgggcttccg acagcttctt ctgcagcttg gcgatgtcgg
     3781 cctcatagcc ggtcagcgcg tcgtcgagga cggcgatctc ggccttgagc ttctcggcca
     3841 tgtcgccggc cttctgcttt tcaacgagcg cggcggtggc aagatcttcg cggtccttcg
     3901 acagcgcgag ttcggccttt tccttccagc tgtcctgcag gctttcgagc ttggcgatgt
     3961 ggcggcgcat ttccttctgg tccgcgatcg tgcgggcggc ggaggcgcgg acttcgacga
     4021 gcgtttcgtt catctcgaag atgatctggc ggatcatctt ttccggatcc tcggcccggt
     4081 cgagcaggtc ggtgacgttg gcggcgatga tgtcgcgggt tcgtgaaaaa atacccattt
     4141 gaaactcctg tcgaaaactt gagaccttgt tgtctgttct taaaaaaggc tggtgccggg
     4201 gcggggcaag gggggagagt gccccggcac cagtgccggt caggccaaag cgctgaccgc
     4261 cggggacatg acagcggcaa ccagcgggcc ggcgggagtg ccggtgacgc tggcggcgac
     4321 ctggggctgg tcgatcacgc cgacgagaat ggccagtgct ccggccgcgc tgagcgcgaa
     4381 ggtggcggct tgggcaaaca tcgtcttcat ggttcaactt tccctggttt tcgctgcacc
     4441 ggtgcgatgt gcgccggttc gttgaaatct ttgcaggagg cgtgccaatt ggacgaaacg
     4501 ccggaaaacc gccgaacttc acaccggaat cggcgggcgg atcgatttgg cttgccaatc
     4561 agcgggaaaa tttgccaatg attgggaatg gagcgcgaaa cgcaattcat cggtcagtcg
     4621 gccgcctttc aggacgcggt cgagcgcgcg agcctcgcgg cgtcgctcga ccgtccggtg
     4681 ctcgtcatcg gcgagcgcgg caccggcaag gaactgatcg ccgagcgcct ccaccgtctg
     4741 tcgacgcgtt gggatcgtcc ttatgtgatc atgaactgcg ccgcgatgcc cgagacgctg
     4801 atcgaaagcg aacttttcgg gcacgaggcg ggcgccttca ccggcgcgac gcgcagccgc
     4861 gcggggcgct tcgaggaagc tgacggcggc actttgtttc tcgacgagct cgcgacgatg
     4921 tcgatgggcg cgcaggagcg actgctgcgc gcggttgaat atggcgaggt cacgcgcgtc
     4981 ggcgcgtcgc gtccgatccg cgtcgacgtg cggatcgtcg cggcgaccaa cgagcatctg
     5041 cccgcgctgg tcgacgacaa tcgtttcagg gccgacctgc tcgaccggct gtcgttcgag
     5101 gtcatcaccc tgcccccgct gcgcgcacgc gagggcgata tcgaggtgct cgccgaatat
     5161 ttcgggacgc gcatggcggc ggtgatcggc tgggacgaat ggccgggttt cggcccgcgc
     5221 gcgctcgcgg cgatggaggg gcacgactgg ccgggcaatg tccgcgaact gaggaacgtc
     5281 atcgaacgcg ccatctatcg ctgggccgac ccgacaaagc cggtcgacgc gctgagcttc
     5341 gacccgttcg cgagcccgtg gcagcccgcg gtgcgcaagg cggcggcgaa agccgaaacg
     5401 cccgccgcgc cgaatgccga tccggcgccg gccacctcca cgccttcggg tccggtcagc
     5461 gacctgcgcg ccgcggtcga cggctatgaa aagacgatcc tcgccgatac gatggcgcgc
     5521 tgccgcttca accagaaggt cgcggccgag gcgctggggc tgagttacga ccagatccgc
     5581 catgcgctga agaagcatgg gctgaatcag taacccggcc cattcttcat cgtcatgccg
     5641 gacttgatcc ggcatccatg acgcggcggc cgagtggatc ccggatcaag tccgggatga
     5701 cgaacggaga ttactctctc ggcaaccccg cgctcagcgc ctcgatcacc gcatcctgcg
     5761 tcgacaccag atcggcgagg cggtcgcgca gcgcatagat gtcgggcagg ccgtcgacct
     5821 gctcgtcgac gctgcggtcg agatagagcc ggtcgatgtc ggcg
//
LOCUS       DATMSU010000396         4687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_110976,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000396 DATMSU010000000
VERSION     DATMSU010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110976"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VN034_10670"
     CDS             complement(<1..346)
                     /locus_tag="VN034_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWV61109.1"
                     /translation="MEHNRSRARVLLLTAALGPLDYRVPQGSEAPLGSVVVAPLGPRR
                     MGGVVWEDESFGAPEPVGDNRLRNLYEIVPVPPIAAPLRRLIEWTSDYYLSPPGSVLR
                     MVLPGAAFADARR"
     gene            536..2890
                     /locus_tag="VN034_10675"
     CDS             536..2890
                     /locus_tag="VN034_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWV61110.1"
                     /translation="MKSLLTDPIAAEEISVRTLLGLGPRNQDLGNLRQLQLAPLRGRG
                     SLRLLMGIGMALVAAFTMVHSVPLSVAGGWLACALVFALWSYGKFNRLPLGDPKSSGI
                     AEYRLCNRHAFYSALLWGALFWLQGPSPALDHVLSMWTIAVLMMVTLAIAAHSVPLAC
                     ALFIAPVSLSAAAALTRAGAPQLAGVALVAGFLLCAFCIRFAQSHVRFRRAEETLHEK
                     TETVSLLLREFEETSADWLWQTDNSRRLIHVSPRLAYALGGSAEGLEGIPLLQALSGD
                     AWETGLFPKSLHDMAERMKRRESFSNLIVPVTIGGAPRWWELSASPRLDEAGKFLGFR
                     GVGSDVTEKRATAEQIAKMARFDNLTGLPNRLSLNEDLARALAHAVEAKSRCALLMID
                     LDRFKAVNDTLGHPVGDKLLAQVAARLKGLMERGMTCGRLGGDEFAVVLHNLPSADAA
                     EDLAQRIITAISRPYVVDNHQLFVGASVGFAIGPTDGATVETLTRNADLALYKSKDKG
                     GNVVAAYVASLHAQAEERRVMEQELRGALERGEFELYYQPVVTAADGTLNGFEALIRW
                     HNKKLGNVSPGRFIPLAEDARLISPIGEWVLRTACHEAMKWPSNLKVAINVSADQLTD
                     PSFASVVVSALAQSGLPPQRLEIEVTESVFLRDGGGAAQLLDQLIALGIRLSLDDFGT
                     GYSSLGYLRKTQFSTIKVDRTFVVGAAKGSIESIAIIRAVVALADSLGMSTTAEGAET
                     ELEVETLRSFGCSNIQGYYYGRPMPASDVLTLFRRPGDVATAAA"
     gene            3099..3632
                     /locus_tag="VN034_10680"
     CDS             3099..3632
                     /locus_tag="VN034_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HWV61111.1"
                     /translation="MTVTGLFASVPAAAKSYLQCVPFARAESGVEIRGNAKTWWSQAA
                     GTYQRGEEPRTGAVMAFAGTRGMPMGHVAVVKKIVGDREILIDHANWSPINGRRGQIE
                     RNVRVVDVSDAGDWSMVRVWYAPIGDLGLRANPVQGFIYADGAPDAAPSFKAPVWTQN
                     EWKPGNGLEVVAASLGN"
     gene            complement(3898..4167)
                     /locus_tag="VN034_10685"
     CDS             complement(3898..4167)
                     /locus_tag="VN034_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HWV61112.1"
                     /translation="MTDTPPDRLSTNPRSPHFDMEVLQRGIGIRFKGAERTDVEEYSI
                     SEGWIRVAAGKSKDRFGQPMTIKLSGPVEAWFEDSAGDGEAPAAD"
BASE COUNT          814 a         1566 c         1491 g          816 t
ORIGIN      
        1 ggcgccgtgc gtcggcgaac gcggcgccgg gcagcaccat gcgcagcacc gatcccgggg
       61 ggctgagata atagtcgctc gtccattcga tcaggcggcg gagcggcgcc gcgatcggcg
      121 gcaccggaac gatctcgtaa agattgcgca ggcgattgtc gccgaccggc tcgggcgcgc
      181 cgaagctttc atcctcccac accacgccgc ccatccgccg cggtccgagc ggcgcgacga
      241 cgacactgcc gagcggcgct tcgctcccct gcgggacgcg atagtcgagc gggccgagag
      301 cggcggtcaa aaggaggacg cgggcgcggg agcgattatg ttccatgggg tcgggcagga
      361 tatagggccg ccggtaaaat aggaaagcgc cgagctcggc ccccgcctgc gcggggggcg
      421 aaggtcatgg caaatgttcg cccgtcccaa tagcgtcccc gggcgggaca gaaattcgcc
      481 ccgcaacagc gcgatggtta ccttcgggta atacccgcgc gctaaacccg cgggcgtgaa
      541 aagcctgttg accgacccga tcgctgccga agagatatcc gtgcggaccc tgttggggct
      601 ggggccgcga aaccaggatc tcggcaacct ccgccagctc cagctcgcgc cgcttcgcgg
      661 ccgcggctcg ctgcgcctgt tgatgggcat cggcatggcg ctcgtcgccg ccttcacgat
      721 ggttcacagc gtgccgttgt cggtcgcggg cggctggctc gcctgcgccc tcgtcttcgc
      781 gctctggtcg tacggcaagt tcaaccgcct gccgctcggc gaccccaaat cgtcggggat
      841 cgccgaatac cggctgtgca atcgccacgc cttttattcg gcgctgctct ggggcgcgct
      901 cttctggttg caggggccgt ccccggcgct cgaccatgtc ctgtcgatgt ggacgatcgc
      961 ggtgctgatg atggtcacgc tggcgatcgc cgcgcacagc gttccgctcg cctgcgcgct
     1021 gtttatcgcc cccgtctcgc tgtcggctgc cgccgcgctg acgcgcgccg gcgcgccgca
     1081 actggccggg gtcgcgctcg tcgcgggctt cttgctttgc gctttctgca tccgtttcgc
     1141 gcaaagccat gtccgcttcc gccgcgccga ggaaacgctg cacgaaaaga ccgagacggt
     1201 cagcctgctg ctgcgcgaat tcgaggaaac ctcggccgac tggctgtggc agaccgacaa
     1261 cagccgccgc ctgatccatg tctcgccgcg cctcgcctat gcgctcggcg gcagcgccga
     1321 ggggctcgag ggcattccgc tcctccaggc gctttcgggc gacgcatggg aaaccggcct
     1381 ctttcccaag agcctgcacg acatggccga gcggatgaag cggcgcgaga gtttctcgaa
     1441 cctgatcgtt ccggtgacga tcggcggcgc gccgcgctgg tgggagctct ccgcttcgcc
     1501 gcgcctcgac gaagcgggca agtttctggg gttccgcggc gtcggatcgg acgtcaccga
     1561 aaagcgcgcg accgccgaac agatcgccaa gatggcgcgc ttcgacaatc tcaccggcct
     1621 gcccaatcgt ctcagcctca acgaggatct cgcccgcgcg ctggcgcatg cggtcgaggc
     1681 gaagtcgcgc tgcgcgctgc tgatgatcga cctcgaccgc ttcaaggcgg tcaacgatac
     1741 gttggggcac cccgtcggcg acaaattgct cgcgcaggtc gcggcgcggc tcaaggggct
     1801 gatggagcgc gggatgacgt gcgggcggct cggcggcgac gaattcgccg tcgtactcca
     1861 caacctgccc tcggccgacg ccgccgagga tctcgcgcag cggatcatca ccgcgatcag
     1921 ccgcccctat gtggtcgaca atcatcaatt gttcgtcggc gccagcgtcg gtttcgcgat
     1981 cggcccgacc gacggcgcga cggtcgaaac gctgacccgc aacgccgacc tcgcgctcta
     2041 caagtcgaag gacaagggcg gcaatgtcgt cgccgcctat gtcgcctcgc tccacgcgca
     2101 ggcggaggag cggcgcgtga tggagcagga actgcgcggc gcgctcgaac gcggcgagtt
     2161 cgaactttat taccagccgg tggtcaccgc cgccgacggc accttgaacg gcttcgaggc
     2221 gctgatccgc tggcacaaca agaagctcgg caatgtctcg ccgggccgct tcatcccgct
     2281 cgccgaggat gcgcgcctga tttcgccgat cggcgaatgg gtgctgcgca ccgcgtgcca
     2341 cgaggcgatg aaatggccgt cgaacctgaa ggtcgcgatc aacgtctcgg ccgaccagct
     2401 caccgacccc agcttcgcct cggtcgtcgt ctcggcgctc gcgcagagcg ggcttccgcc
     2461 gcaaaggttg gagatcgagg tcaccgaaag cgtcttcctg cgcgacggcg gcggcgcggc
     2521 gcaattgctc gaccagctga tcgcgctcgg gattcgtctg tcgctcgacg atttcggcac
     2581 cggctattcg tcgctcggtt acctccggaa aacgcaattc tcgacgatca aggtcgaccg
     2641 gaccttcgtc gtcggcgccg cgaagggcag catcgaatcg atcgcgatca tccgcgccgt
     2701 ggtcgcgctt gccgacagcc tcggcatgtc gacgaccgcc gagggcgccg aaaccgagct
     2761 cgaggttgaa acgctgcgca gcttcggctg cagcaacatc cagggctatt attacgggcg
     2821 cccgatgccc gcgagcgacg tgctgaccct gttccgccgt cccggcgacg tcgcaacggc
     2881 agccgcatga cggcggccag ccgaatgtgc tgcacgacga attgaacgtc ttaacaaaag
     2941 gttcccccgt ccaccgcatc cccggcgcga ttcgtcgctt cgcgggtggt ggacgccgcg
     3001 cggcgtggca aagaccctcc aacgcttgaa gacaagcatc gggggggtcg catttcatgc
     3061 tccatcgtcg ttacctggca gccgccatcg cgtccgtgat gaccgtcacc ggcctgttcg
     3121 cgagcgttcc cgccgcggcg aaatcctatt tgcaatgcgt ccccttcgcc cgcgccgaat
     3181 cgggcgtcga aatccgcggc aatgccaaga cctggtggtc gcaggcggcc ggcacctatc
     3241 agcgcggcga agagccgcgc acgggcgccg tcatggcctt cgccggcacg cgcggcatgc
     3301 cgatgggcca tgtcgcggtg gtcaagaaga tcgtcggcga ccgcgaaatc ctgatcgacc
     3361 atgccaactg gtcgccgatc aacggccgcc gcggccagat cgaacgaaac gtccgcgtcg
     3421 tcgacgtcag cgacgccggc gactggagca tggtccgcgt atggtacgcc ccgatcggcg
     3481 acctcggcct ccgcgccaac ccggtgcagg gcttcatcta cgccgacggc gcccccgatg
     3541 cggcgcccag cttcaaggcg cccgtctgga cgcaaaatga atggaaaccc ggcaacggcc
     3601 tcgaagtcgt cgccgcctcg ctcggcaact gagccttccc cccccccacc ctcgggctcg
     3661 gcgagcgatg aagccactcg gggcggtccg ccgctccggg tggtttctcc tttggggcgt
     3721 cgtgcgttaa actggcagta tcagccgatc cgttcgccct gagcttgtcg aagggctgtt
     3781 cttcttttcg cccgtgctgc aagaaagaac ggtgcttcga caagctcagc acgaacggtc
     3841 ttcaacgggt gccgatcaaa gactcgatgc tctggcccga gttgggctcc actcgactca
     3901 atccgcggcg ggtgcctcgc catcgccggc gctgtcctcg aaccaggctt cgaccgggcc
     3961 cgacagcttg atcgtcatcg gctggccgaa acggtccttc gacttgcccg cggcgacgcg
     4021 gatccagcct tcggagatcg aatattcctc gacgtcggtg cgctcggcgc ccttgaagcg
     4081 aatgccgata ccgcgctgca gcacctccat gtcgaaatgg ggcgagcgcg gattggtcga
     4141 caggcggtcg gggggcgtat cggtcatgac tagtatccca aaaaatgatg gcgcggccct
     4201 agcgggaccg cagccgcatc gtcaatggag aggtgaagcg ctgcggcgcg cccgtgcgca
     4261 aggtctgcgc ataccgctgt tcgcgaaaac cccgttgcgg cccggcttcg tcgagatgcg
     4321 gccggcgagg tgagatggcg ccgacgcgcg gcggatatcc ttttctcgat tttgcgccct
     4381 ccggcgaagc cgcaagtcgc ccatcgaagc agggaacctt tataattcaa atcaatttaa
     4441 ggaaattttg tccccgatcg aaacagatgc gctagtatca tataattgaa gccaactcct
     4501 ctgtaagaaa taaattagtt aactcaagat cgattatccg ccggaaaccc catatattag
     4561 ttaaaatagt aactttgttc aaatgaatct gaacaataga aggaactttg tcgacatttc
     4621 aagaatctca agatcaggga ccagatcatg tacgaagggg atgtctcatg cctttgtcct
     4681 gtcttga
//
LOCUS       DATMSU010000397         1672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_130334,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000397 DATMSU010000000
VERSION     DATMSU010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130334"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..660
                     /locus_tag="VN034_10690"
     CDS             181..660
                     /locus_tag="VN034_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV61113.1"
                     /translation="MQGDPDNYLSLLSAAQIETLRHVYEHKNSKQIARLMNVSPHTVD
                     ERVRRVLKKLNVSNRIEAARILAVNGVFDDVTPYQPLTYQLIDLGEVPPAVDAEAGRS
                     SFRRFFDIGSPFPTASQPANRHGLMERIIWPILIAVATILAFSALYSILVGLGRVLT"
     gene            719..1093
                     /locus_tag="VN034_10695"
     CDS             719..1093
                     /locus_tag="VN034_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61114.1"
                     /translation="MLNQRVSAAKKIASELHLAEDAIDEAMIRIAQLAATLPTARRET
                     NMSAIVGQEAMAKVAQALAAAGEVRQLLTDAHLALTVTQKEVGLGTRMFGAGVKPASA
                     KLVDEGSNDRQGADAPAFAKAG"
     gene            1119..1532
                     /locus_tag="VN034_10700"
     CDS             1119..1532
                     /locus_tag="VN034_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61115.1"
                     /translation="MWSVAIYYAVLLITVAVAIRRGGPLERWAAYTALIASVLTTAVT
                     PFPTWTNIEVNIFIIDVLVLMSFWFIAMRTQSFWPYWITGWQLIAIFGHIQKLMFSEI
                     LARPYSLLSVYISYPILLLIIYASGSYSRRNEVTA"
BASE COUNT          329 a          505 c          502 g          336 t
ORIGIN      
        1 cgccgcgcgg cgcgccgcgc gccggcacat cgaaaagtcg atccagcgct atcggacgct
       61 caaggagagc cgctagcgcg ggcgaatcgg gcgccgcagc ggtggccgaa tatgggtcgg
      121 acgggaaatt agctatctac cgaccttgat ttcgactcgt gatgctctat tccaacccgc
      181 gtgcaaggcg accccgacaa ttacctcagc ctgctgtccg ccgcgcagat tgaaacgttg
      241 cggcacgtct acgagcacaa aaattccaag cagatcgcgc gcctgatgaa cgtgtcgccg
      301 cacaccgttg acgaacgtgt ccggcgcgtt ctaaaaaaac tgaatgtctc caataggata
      361 gaggcagcgc gaatcctggc tgttaacggt gtcttcgacg atgttacccc ttatcagcct
      421 ttgacatatc aattgatcga cctaggcgag gttcccccgg ccgtcgatgc cgaagcgggg
      481 cgcagttcgt ttcggcgatt tttcgacatt ggttcgccat ttcctaccgc gtcccagccg
      541 gccaaccggc atgggttgat ggaacggata atctggccga tcctgatcgc agtagcgacg
      601 attttggctt tctccgccct ctattcgatc cttgtcggac tcggtcgcgt tctgacctga
      661 agttcgaacg aatgctgcgg gggggaaccg gtagcattct ttttcaagga aaaagatgat
      721 gctgaatcaa cgagtttctg cggcgaagaa gatcgcttcc gagctgcatc tggccgaaga
      781 cgccatcgat gaggcgatga tccgcatcgc ccagctcgcc gcgacgctgc ccaccgcgcg
      841 ccgcgagacg aacatgtcgg cgatcgtcgg ccaggaagcg atggcgaagg tcgcgcaggc
      901 gctcgccgcc gccggcgagg tccgtcagct gttgaccgat gcgcacctcg cgctgaccgt
      961 gacgcagaag gaagtcggcc tcggcacgcg catgttcggc gctggcgtca agccggcgag
     1021 cgcgaagctg gtcgatgaag gcagcaacga tcgtcagggc gccgacgcgc cagcgttcgc
     1081 gaaggccggc tgatccgcaa gaaaggttga ctgccacaat gtggtccgtg gcgatctatt
     1141 atgccgttct gctcatcacc gtggccgtcg cgatccgacg cggcggaccg ctcgaacgct
     1201 gggcagccta tacggcattg atcgcctcgg tcctcacgac ggccgtgacg cctttcccga
     1261 cctggacgaa catcgaggtc aatatcttca tcatcgacgt actggttttg atgagcttct
     1321 ggttcatcgc catgcggacg cagagtttct ggccttactg gatcaccgga tggcagctga
     1381 tcgccatttt cgggcatatc cagaagctca tgttctcgga gatattggcg cggccctatt
     1441 cgctgctctc ggtgtatata tcctatccga tcctgttgct gatcatctac gcatcggggt
     1501 cctacagtcg gcggaatgag gttacggcct gagatgtggc tccccaggga catcgtcaca
     1561 tgcagccatt ttcgaatgaa gaaatcgccg agctgaaatc gcgggtcgac cagatccacg
     1621 agctgctcgc gagccgcggc gaggaaacgc cgcccgccgc cgcgcgcggc gg
//
LOCUS       DATMSU010000398         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_25591, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000398 DATMSU010000000
VERSION     DATMSU010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25591"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VN034_10705"
     CDS             <1..231
                     /locus_tag="VN034_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWV61116.1"
                     /translation="ADRVRDHLASVDLPHSLASAGVNTGGAELAAHMAHDKKVRGGKL
                     PLILTRGIGQSFVTDEYGLDRVAAFLDGQRAA"
     gene            complement(242..2146)
                     /locus_tag="VN034_10710"
     CDS             complement(242..2146)
                     /locus_tag="VN034_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWV61117.1"
                     /translation="MLTALAAGASEAAAKVADTATDTALIEGAILLGVATLFVLIFRR
                     LGLGAVLGYLIAGAIVGPHGLGLVGGGESKLAFAELGIAFLLFLVGLELSPRRLWDMR
                     RAIFGLGMAQVVVTGLVLTALIHLTLGFSPAASLALGLPLALSSTAQVLPGLKSSGRI
                     NSPFGEKAFSILLFQDLAIVPMITIVAVLSRAPANPSAPPGWQMAFFTLWAIVVLVLA
                     GRFIVNPLLRIVGRYGERELFVVVGLLTILASAALMHSLHLSTALGAFIAGVMLADSP
                     YRHEIESDVEPFRLILLGLFFLAVGMVLDVGAVMERPLLVLALALGLVAVKAAVLTLI
                     VKAFGKSWQAALGLGLLLSQGGEFGFLLFAQAADALLIDPEAASLFSAVVTLSMVTTP
                     FLMLFARNLEFSPGADEPDLDDPEGAPRGSAIVVGYGRFGQIVSQMLHGVACSVTLID
                     KKPSQIELSSRFDTKVYFGDGLRVDLLRRAGAEEARLIIFCMDDSSVDAAAMRPIVEA
                     FPNAKVLMRVFDRRQLLAIDGAGVDAVIREVFESSIAMGLTALRHLDVDPSEMDDVEA
                     TLRHLDETRLQAQIDEGDISAGMDHRFKPGGGREADSVLEKFRRKRQAAKAAREEREE
                     NEAMGLQADS"
BASE COUNT          419 a          735 c          697 g          301 t
ORIGIN      
        1 gccgaccgcg tgcgcgacca tcttgcgagc gtcgacctgc cgcacagcct ggcgagcgcg
       61 ggcgtgaaca ccggcggcgc cgaactcgcc gcgcatatgg cgcacgacaa gaaggtgcgc
      121 ggcggcaagc tgccgctgat cctgacgcgc ggcatcgggc aaagcttcgt caccgacgaa
      181 tatggtctcg atagggtggc ggcgttcctc gacggacagc gcgccgcata aagcgtttcg
      241 actaactgtc ggcctgcaag cccattgcct cattttcttc gcgttcttcg cgagccgctt
      301 tcgccgcctg ccgcttacgg cggaattttt cgagcacgct gtcagcctcg cgcccgccgc
      361 cgggcttgaa gcgatggtcc atccccgccg aaatatcgcc ttcgtcgatc tgcgcctgca
      421 gccgcgtctc gtcgagatgg cgcaaggtcg cctcgacatc gtccatctcg ctcgggtcga
      481 catcgagatg gcgcagcgcg gtgaggccca tcgcgatcga actttcgaaa acctcgcgaa
      541 taaccgcgtc gaccccggcc ccgtcgattg cgagcaactg acggcggtcg aatacgcgca
      601 tcaggacttt ggcattcgga aaggcctcga cgatcgggcg catcgccgcg gcgtcgaccg
      661 aggaatcatc catgcagaag atgatcagcc gcgcttcctc ggcccccgcg cggcggagca
      721 ggtcgacgcg cagaccgtcg ccgaaataga ccttggtgtc gaagcggctc gacagttcga
      781 tctggcttgg cttcttgtcg atcagcgtga ccgagcaggc gacgccgtgc agcatctgcg
      841 acacgatctg tccgaaccgt ccatatccga cgacgatcgc cgacccgcgc ggcgccccct
      901 cgggatcgtc gagatcgggc tcgtcggctc cgggcgagaa ttccagattg cgcgcgaaca
      961 gcatcaggaa cggcgtcgtc accatcgaca gcgtcaccac cgcgctgaac aggctcgccg
     1021 cctcggggtc gatgagcagc gcgtcggcgg cttgcgcgaa aagcaggaaa ccgaattcgc
     1081 cgccctggct gagcaacaga ccgaggccca gcgcagcctg ccagctcttg ccgaacgcct
     1141 tcacgatcag cgtcagcacg gccgccttca ccgccaccag cccaagcgcc agcgcgagca
     1201 ccagcaacgg acgttccatg accgcgccga catcgagcac catgccgacg gcgaggaaaa
     1261 agaggcccag caggatcagg cggaagggct cgacgtcgga ttcgatttcg tgccgatagg
     1321 gcgaatcggc gagcatcacg ccggcgataa acgccccgag cgcggtcgac aggtgcaggc
     1381 tgtgcatcaa ggccgcgctc gcgaggatgg tcagcagccc gacgacgacg aacagctcgc
     1441 gctcgccata gcggcccacg atgcgcagga gcgggttgac gatgaagcgc cccgcgagca
     1501 ccagcacgac gatcgcccac agcgtgaaaa aggccatctg ccaccccggc ggcgccgatg
     1561 gattggccgg tgcgcgcgac agcaccgcga cgatcgtgat catcgggacg atcgcgagat
     1621 cctggaacaa taggatcgag aaggcttttt cgccgaaagg cgaattgatc cgccccgagc
     1681 ttttgagccc tggcagcacc tgcgcggtcg acgacagcgc gagcggcagg ccgagcgcga
     1741 gcgaagccgc gggactgaat cccagcgtca gatgaatgag cgcggtgagc accagccccg
     1801 tcacgacgac ctgcgccatg ccgagcccga agatcgcgcg ccgcatgtcc cacaggcggc
     1861 gcggcgagag ttcgagcccg acgaggaaga gcaggaaggc gatgccgagt tcggcaaagg
     1921 ccagcttcga ttcgccgccg ccgacgaggc cgagcccgtg cggcccgacg atcgcccccg
     1981 cgatcagata gcccagaacg gcgccgaggc cgagtcggcg aaagatcagc acgaacaggg
     2041 tcgcgacgcc gagcaggatc gcgccctcga tcagcgccgt gtcggtcgcg gtgtcggcca
     2101 ccttcgcagc cgcttcggag gcaccggcag cgagtgcggt cagcatcacg cc
//
LOCUS       DATMSU010000399         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_16179, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000399 DATMSU010000000
VERSION     DATMSU010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16179"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1200
                     /gene="pdhA"
                     /locus_tag="VN034_10715"
     CDS             130..1200
                     /gene="pdhA"
                     /locus_tag="VN034_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HWV61118.1"
                     /translation="MAKAPARTSPAPKKTASTPAAASNREQPRDPVPYDATPEELEKF
                     YREMLLIRRFEEKAGQLYGLGLIGGFCHLYIGQEAVAVGLQSALDGDKDSVITGYRDH
                     GHMLAYGIDPKVIMAELTGRQAGISKGKGGSMHMFSVEHKFYGGHGIVGAQVSLGTGL
                     AFAHKYRGDGGVAMAYFGDGASNQGQVYESFNMAELWKLPIIFVIENNQYAMGTSVNR
                     SSAEDQLYRRGESFRIPGMQVDGMDVLAVRGAAEAALEWVRAGKGPVLMELKTYRYRG
                     HSMSDPAKYRSREEVQAVRDKSDSIEHLKKLMTDAGIGEDKFKEIDKEIRAIVAESAD
                     FAESAPEPELHELYTDVLVEQY"
     gene            1202..2611
                     /locus_tag="VN034_10720"
     CDS             1202..2611
                     /locus_tag="VN034_10720"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HWV61119.1"
                     /translation="MAIELKMPALSPTMEEGTLAKWLVKEGDTVKSGDILAEIETDKA
                     TMEFEAIDEGTIGQILVAEGTDNVKVGTVIATITGEGEEAAAPAPAPAAAPAKDVAPA
                     EAGAAVASAPTPAVPASAGTTEKAAPAERASDPAIPEGTAMVKLTVREALRDAMAEEM
                     RKDDRVFVMGEEVAEYQGAYKVTQGLLQEFGAQRVVDTPITEYGFAGLGAGAAMGGLK
                     PIIEFMTFNFAMQAIDHIINSAAKTNYMSGGQMRCPIVFRGPNGAAARVGAQHSQNYA
                     PWYAAVPGLIVIAPYDAADAKGLMKAAIRTEDPVVFLENELLYGRSFEVPDVEDFVLP
                     IGKARTMRKGSDVTVVSYSIGVGLALEAADQLAGEGIDAEVIDLRTLRPLDTATVLAS
                     LKKTNHLVIVEEGWPVCSIASELAMVAMEHGFDDLDAPVMRVCNEDVPLPYANNLEKA
                     ALIDTPRVVKAVKAVLNRA"
BASE COUNT          568 a          912 c          928 g          455 t
ORIGIN      
        1 aggcatgcgc ggactcatga tgcatacgta agttggcata gcggccgccc atggtgggcg
       61 ttgccaagtg ccgcgcggct ctcctatagg gagcgcttgc cgtaacggct aactgcaaag
      121 gaacccgcct tggccaaagc acccgcgcgc acctcccccg cgccaaaaaa gacagcatcc
      181 accccggccg ccgcgagcaa ccgcgagcaa ccgcgcgatc ccgttcccta tgacgcgacg
      241 cccgaagagc tggaaaaatt ctatcgcgag atgctgctca tccgccgctt cgaagagaag
      301 gcggggcagc tttacggcct cggcctgatc ggcggcttct gccacctcta tatcggtcag
      361 gaagccgtgg cggtcggcct gcagtcggcg ctcgacggcg acaaggacag cgtgatcacc
      421 ggctaccgcg accatggcca catgcttgcg tacggcatcg accccaaggt catcatggcc
      481 gagctgacgg ggcgccaggc cggcatctcg aagggcaagg gcggctcgat gcacatgttc
      541 agcgtcgagc ataaattcta tggcggccac ggtatcgtcg gcgcgcaggt gtcgcttggc
      601 accggcctcg ccttcgcgca caagtaccgc ggcgacggcg gggtcgcgat ggcctatttc
      661 ggcgatggcg catcgaacca gggccaggtc tacgaaagct tcaacatggc cgagctgtgg
      721 aagctgccga tcatcttcgt gatcgagaac aaccaatatg cgatgggcac ctcggtcaac
      781 cgctcgtcgg ccgaggacca gctctatcgc cgcggcgaaa gcttccgcat cccgggcatg
      841 caggtcgacg gcatggacgt gctcgcggtg cgcggcgccg ccgaagcagc gctcgaatgg
      901 gtgcgtgcgg gcaagggccc ggtgctgatg gagctcaaga cctatcgtta ccgcggtcac
      961 tcgatgtccg atcccgccaa atatcgcagc cgcgaggaag tgcaggcggt gcgcgacaag
     1021 agcgattcga tcgaacatct gaaaaagctg atgaccgatg cgggcatcgg cgaagacaag
     1081 ttcaaggaaa tcgacaagga aatccgcgcg atcgtggccg agtccgccga ctttgcggaa
     1141 agcgcgcccg aacccgaact tcatgaactg tataccgacg tgctggtgga gcaatattga
     1201 gatggccatc gaattgaaaa tgccggcact gtcgccgacg atggaagaag gcacgctcgc
     1261 caagtggctc gtcaaggaag gcgacacggt aaagtcgggc gacattctcg ccgagatcga
     1321 aaccgacaag gcgacgatgg aattcgaagc gatcgacgaa ggcacgatcg gccagatcct
     1381 cgtggccgag ggcaccgaca atgtgaaggt cggcaccgtg atcgcgacga tcaccggcga
     1441 gggcgaggaa gcggccgcac cggcaccggc accggccgcc gcacctgcca aagacgtcgc
     1501 cccagcggaa gctggggccg ctgtcgccag cgctcccacg cccgcggtcc cggcttccgc
     1561 tgggacgacg gagaaggcgg cacccgccga acgcgcctcc gaccccgcga ttcccgaggg
     1621 caccgcgatg gtgaagctca ccgtccgcga agcgctgcgc gatgcgatgg ctgaggaaat
     1681 gcggaaggac gaccgcgtct tcgtgatggg cgaggaagtc gccgaatatc agggcgcgta
     1741 caaggtcacg cagggtctgc tgcaggaatt cggcgcgcag cgcgtggtcg ataccccgat
     1801 caccgaatat ggcttcgcgg gtctcggcgc gggcgcggcg atgggcggtc tgaagcccat
     1861 catcgaattc atgaccttca acttcgcgat gcaggcgatc gaccacatca tcaactcggc
     1921 ggcgaagacc aattatatgt cgggtggcca gatgcgctgt ccgatcgtgt tccgcggccc
     1981 caacggcgcc gcggcgcgcg tcggcgcgca gcacagccag aattatgcgc cctggtacgc
     2041 cgccgtcccc ggcctgatcg tgatcgcgcc ctatgacgcc gccgatgcca aggggctgat
     2101 gaaggcggcg atccgcaccg aggaccccgt cgtcttcctc gaaaacgagc tgctctatgg
     2161 ccgcagcttc gaggttcccg acgtcgagga cttcgtgctg ccgatcggca aggcgcggac
     2221 catgcgcaag ggtagcgacg tcaccgtcgt cagctattcg atcggcgtcg ggctcgcgct
     2281 cgaagccgcc gaccaactgg cgggcgaggg gatcgacgcc gaagtgatcg atctccgcac
     2341 gctccgcccg ctcgacaccg cgacggtgct cgcgagcctc aagaagacga accacctcgt
     2401 catcgtcgag gaaggctggc cggtctgctc gatcgccagc gaactcgcga tggtcgcgat
     2461 ggagcatggc ttcgacgatc tcgacgcgcc cgtgatgcgc gtgtgcaacg aggatgtgcc
     2521 gctgccctat gcgaacaatc tcgaaaaggc cgcgctgatc gatacgccgc gcgtcgtgaa
     2581 ggccgtgaag gcggtcctca atcgcgcctg aggcttcgcg ccaactttaa ttccgttcgt
     2641 gctgagcctg tcgaagcacc gttcttccca ttgaagagaa gagcggccct tcgacaggct
     2701 cagggcgaac ggcttttaat ttgtgcgttc tgctctaacg ctgtctttgc acaatgagcg
     2761 aaaccgaaac tttctatccc gacatgcgcg atccgcgcgt catggtcgcg gtgccgcagg
     2821 cgcgacgccc ggccatggcg gcgctgtggg ggctcgccgc gcg
//
LOCUS       DATMSU010000400        23604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_158609,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000400 DATMSU010000000
VERSION     DATMSU010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 23604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23604
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158609"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2478)
                     /locus_tag="VN034_10725"
     CDS             complement(<1..2478)
                     /locus_tag="VN034_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61120.1"
                     /translation="MGMKRVAAVRAGLRGGSALFLSMAVMGSAMAQEPVAAEEGAIVV
                     TGSRIPRPDVESNSPVNVIGQDEIKYSATVETEQLLNALPQAVAGAGAQSNAGNGSAT
                     VNLRNLGTVRTLVLQNGRRIVGASQDGVVDLNMIPPSLVARVEVVTGGASAVYGSDAM
                     AGVVNFVTKDDFEGFEIGGSYGISDEGDAERYNLELTVGGNFADGRGNMVFHGSYYDR
                     AMVKGAARDHAVEYFADAIVDGKPVLVPAGNGVTPQGTIFSPQLVGLTDPYGNTIGTA
                     GIFFADEGWRAYKPSDGFNDRPFTNIQMPMQRWQGSVIGHYDVTDDVTAFWEGSYVHS
                     KINSTLGAVPMSSSGFIPGFQLDLRNPYLDPTLRDFLSTNMDADGNSLVPVNINRRLL
                     DGGSRTSDQTRDFYRFVVGLKGNLTDRLKWEVFYNQGQTKVTDVQGGGVLIDNFANGF
                     LTDPNDPYKCAVADPECVVLNPFGFNSLTPEMIDYIYTDLTNITTIRQKQVGASLTGS
                     LFTLPAGDVGISIGAEYRKESSSFDPDQLYVQGKALSRSAGLSPTAGSFDVSELYGEL
                     YVPILADMPGVELLAFEGGLRFSDYSTAGSVWSYKLGGEYSPFRGLKFRGLYQRAVRA
                     PNVVELYSGATNTAPQALDFCNAGADRTQQERDFCVNSLGIPAAVIDVFQQENQQIRA
                     ITGGNPNLQEETSDTWSVGAVIRPEFIPNLQLTVDYYNIKIDDAIGSFGGGLQSVITA
                     CNADMSLSNIFCQPLRNRTPDGQLYDVPLLNANIAKMEASGIDYRLDYRHSLGSAGSL
                     NYYIAGTYLIDAITQGSPIVSPVNCAGY"
     gene            2940..4643
                     /locus_tag="VN034_10730"
     CDS             2940..4643
                     /locus_tag="VN034_10730"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HWV61121.1"
                     /translation="MRKAFVVAITALIASTTSLTPLAAAGAAEQVSTVQATPPDGKGS
                     YDELVTLFTAFNEWKTPRAIDGVVDYSPAAVEKRRTELRAFQARLPDFAVARWDRHKQ
                     VDYLAVRAQMDLADFTLNVTKPWARDPGMYVDELQRLAYTDLPLEGEALTTFRARLRT
                     IPAYLAQARINLDSVAADYADLAIHSLSHGDGVGHGMPYRPVEPEGLIGWFADLHGRA
                     AKQQPALLPDIEAAEQAITGFRDWLVDARPTMTVSAGVGKPAYDWFLTNVRLMPYDSD
                     QIVTLAQRELERHWANYTTERHRNRNLPELELPRSAEEYEAQLAGTDAKIRKWLKDEE
                     IITIPDYIPATYQEVGFNAPWIVRPHGPMFWEQVQFRDASPDHLHATFPGHRFDGMTG
                     KRVSHPIRRHISDSGRTEGWGFYLEENALQLGLFDDRPRTRELIYIFGLFRAARTIGD
                     VKMQRNQMTVDQTMKFWKEWTPYLDDNVARVDAGIYIRRPPGYGMTYTIGAMQLQKLL
                     VDRKRQLGDKFSIRDYHDYLMNTGRLPVSLLRYDLTGYDDEIKKLWRHEPLTTDVRHK
                     P"
     gene            4925..6100
                     /locus_tag="VN034_10735"
     CDS             4925..6100
                     /locus_tag="VN034_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV61122.1"
                     /translation="MRRFDWQDGLSISDRLDEQERMIATAARAYCSDRLHPRVRDAYA
                     REHCDPDIFREMGEQGLLGVTVPEEYGGIGASYVAYGLVAREVERVDSGYRSMLSVQS
                     SLVVHPILAYGSDAQKRRYLPALVAGTSIGCFGLTEPDAGSDPGAMRTRATAVDGGYR
                     IDGNKTWISNAPIADVFIIWAKSEAHGGAIRGFILEKGMAGLSAPKIEGKLSLRASVT
                     GMINLDGVIVPEAALLPHVEGLKGPFGCLNRARYSIAWGAMGAAEACYAAARQYGIDR
                     QQFGRPLAATQLFQKKLADMATEIALGLQAALQVGRLMDEDAVAPEMISLIKRNNVGK
                     ALDIARAARDMHGGNGISGEYPVMRHAINLETVNTYEGTHDIHALIIGRAITGLAAF"
     gene            6102..7238
                     /locus_tag="VN034_10740"
     CDS             6102..7238
                     /locus_tag="VN034_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWV61123.1"
                     /translation="MSSPARTPLAGLKVVELARILAGPWAGQILADLGAEVIKVESPD
                     GDDTRKWGPPFVDNGDGTADAAYFHCANRGKTARAIDFSSPAGKTELIELIRDADVLI
                     ENFKVGTLARYGLDHASLAAINPRLIYCSITGFGQTGPYAGRAGYDFVVQGMSGIMDL
                     TGMPDAPPQKIGVAYADIMTGLYAVIAIEAALIQRATTGTGQHIDMALLDVMVGTLAN
                     QGLNYLASGISPSRLGNAHPNIAPYEAFRCADGWVILAVGNDRQFARLVDALALDERP
                     EWQSNPGRVADRAALSAAITAKLDRWKSRELLAMMDELGVPAGPINRVEDAFADPQIL
                     HREMRLALDREDGAAIPGIRTPIRFSDASLSCNRPAPRLPRSMS"
     gene            complement(7304..8680)
                     /locus_tag="VN034_10745"
     CDS             complement(7304..8680)
                     /locus_tag="VN034_10745"
                     /inference="COORDINATES: protein motif:HMM:NF005682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HWV61124.1"
                     /translation="MEAGFSTDRWRELDDRHHLHPFSDPRELAERGTTVITRGDGSYV
                     WDSDGNKILDGMAGLWCVNVGYGRDELVDVAARQMRELPYYNSFFQSATPSQISLAKR
                     LSEVTPEGIDNFFFTNSGSEANDTVIRLVYHYWKLCGQPSKRVFIGRNLGYHGSTLAA
                     TSLGGMKAMHDMGHELLPGFEHIMEPHWYMHGVNQTPEQFAEVAAAALEDKIAAIGAA
                     NVAAFIAEPVQGAGGVIDPPPGYWERIQKICRKHDILLVADEVICGFGRLGEWFGSQY
                     YGIEPDLMPMAKGLSSGYLPIAAVGIGERVHKTLYEGGAIPHGYTYSGHPVACAVAEA
                     NIDIIAREGLVDRVREDVGPYFRTVLQQIADFHPLVGELRGAGLLAGLQLMKDRTERL
                     FFDPSEKAAVTCREIALRSGLIARAVGHAMVLCPPLTITRGEIDELADKMTKSLDETA
                     KEYGVEAR"
     gene            complement(8799..9446)
                     /locus_tag="VN034_10750"
     CDS             complement(8799..9446)
                     /locus_tag="VN034_10750"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWV61125.1"
                     /translation="MEEQEARHRMFVPEHYRAPDPRALVARYPFAQLVSAASDGAPFA
                     TSVPIYFASDAPDEIVLVGHLARANPHAAVLSDGQSALAIFAGPHAYISSSWYVERPT
                     VPTWNYMTAQVRGRLTPIDDDAEQLAIMRRTIAMSERYNGSDWTMEDAPEGKVEFLLP
                     MIRSFRVAIERIDGVTKLSQTHPPGDRQRVIAALRGRGAAEDLQIADLMENLDRD"
     gene            9644..10777
                     /locus_tag="VN034_10755"
     CDS             9644..10777
                     /locus_tag="VN034_10755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWV61126.1"
                     /translation="MSSQPSDIVDAYAGKLFPGEQFATFSATEKSFPTRTVSRGTGDV
                     SALRPAATTLPDLPIVTGTESCDLIDYVSRNRLVGLTILKGGEIVHERYEGGLTPEMR
                     WISMSMAKSVATTLIGAAIHDGYIGGVDDPLTEYLPVLRGSGYDGVSIRHLMRMTSGV
                     EWDDTHTDPRSHRRHMLGLQVGGQHGAIMDYMATLPRIAEPGSVWNYSTGETHVVGAL
                     VKAATGKWLADYLSEKIWSRVGMQSDAYWWLEAPDGLEVAGSGLCATMRDYVRFGLFA
                     LNGGVAGGERVVPEGWFAEAGGAEHRGGDAFNYGYMWWPVPDRSGSFGDGAYSARGIF
                     GQYIYINPARSILVTVLSSRAKPKFSEAIPDNDFFNAVVDAIG"
     gene            complement(10778..11782)
                     /locus_tag="VN034_10760"
     CDS             complement(10778..11782)
                     /locus_tag="VN034_10760"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWV61127.1"
                     /translation="MNAPYLFDCGRLDMSAPPRPPERKSLPPFEALRAFDAVARLGGI
                     RKAAAWLSRDHAVVSRHLRSIETWLGVQLVARAPGGIVLTEQGRIYHARISQALEDIA
                     HATLDVLNSGQHNSLSIWCTPGFALHWLSQHFAAFEARNTGVDFELRPTDASPDFAGH
                     EADLDIRFNATYEEVAAPSAAERQGTIAHVPIIAVASSRYLEARDAIVEPADLLSHEL
                     LHESGTNNWVQWLRSYGVGCEERLGGPRLWQGHLTVDAARHGRGISLANSLIIRDDLQ
                     SGALIDIGAGKDSFPVRMGHYVLIARRDRWDDRLVRTFRRWLRETIAKELPEFAPGRS
                     "
     gene            12016..13419
                     /locus_tag="VN034_10765"
     CDS             12016..13419
                     /locus_tag="VN034_10765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HWV61128.1"
                     /translation="MTTASSAPLYPASGAADDGKEIFGHPRGLAYIVFTETWERFSFY
                     GMQALLILYMTLHLFKPENSAAVAGFEGYRAIMATIFGPLSTQALAAQTFGLYVGFVY
                     FMPVFGGLLGDRYLGRKNSVIIGAVFMAAGHFLMAFEALFLFALLALVIGSGFLKGNL
                     AAQVGYLYAANDRRRDAGFSIYVFGINVGAFVAPLICGTLGELYGWHYGFGAAGIGML
                     IGLAIYLKGRRYLPPERIRLQQGEQVRLQPGDGTKIAAIAAMLAITALYWTAQSQVWN
                     SYPLWIKDQVDRNLGFATVPVTWFQSLDSLAVLLLAPAVLWFWKRQSRRAAEPADLAK
                     IGLGCAVFATACLLLSVGEILAGSGQVAIVWPVLFHFVCAIGFLYLGPIALALTSRAA
                     PPAVNAMMVGCYYLAIFAGSFASGWLGRFYETMPPAAFWAMHGAIVGTGALLILAFGR
                     PLLRAMAIQTQADEGAQ"
     gene            complement(13847..15973)
                     /locus_tag="VN034_10770"
     CDS             complement(13847..15973)
                     /locus_tag="VN034_10770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61129.1"
                     /translation="MKATFYTGSAVALLAAALAQPAFAQGGGAEGDDEMIVVTAQKRE
                     QKLVDVPMAISVLGGEELAEKGVASVQDLSFSVPGLTMREDGPGSYTIFMRGLANQSG
                     SGALVGQYLDEAPLSLGGFTQLSPVALDLERIEVLKGPQGTLYGQGAAAGVIRYITNK
                     PDLDAFSAAVTGEVYDVDQGDVGYKTSAVVNVPIATGKAALRVAALYEDGGGWIDQPE
                     AGIKDGNGTELLNVRAQLLLQPTDRFSAKAMVQIHRAETRLGLGFEEPDRTVDIGIDR
                     ATVLIPKKFDFTTYNLELNYDLDFAQLVSATTYTEFDEQYPFTYIPRPGNFSFGFVEG
                     SDARYGHVSQFSQELRLASASGPFQWTVGGFYALGKDRGSADYGYVFAENGDLYENGG
                     VLIDNLYFFSKGKTENYSIFADLSYDLTDRLTIGGGLRYFRDKQRDLSERVPGAGVTK
                     RASFESTDPRVVVSYRFADNARLYASYGQGFRSGGFNEDPFGPYGPESVETYEVGSKG
                     SFGGGLFQYEVAGFITNYSDMIRRRLVNVDGLLLPESSNIGKARVKGLEFGVQVRPAT
                     GLTFGVNAAYLDSKIVDTDPGDQVNIIGDLTDYTPKLSYSASANYDFAVSDSVDAFVR
                     VDFNFRDKVTYTDRSSFFANVLPQKSDTLSLLNARLGFDFGRFNAEIYGSNLLNINRA
                     IDPYQGWANSNRTRPRVIGLKLGAKF"
     gene            complement(16197..17483)
                     /locus_tag="VN034_10775"
     CDS             complement(16197..17483)
                     /locus_tag="VN034_10775"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWV61130.1"
                     /translation="MTLNDGAGTKDALSFDWPMLSIGTGQYEDGPTGLTVIRFKERVH
                     AAIDTRGGGPGTVNSDYLRLGYEFPELDAIVLAGGSWYGLEAVTAVNTALKDDGARGG
                     HWDNVGLVEGSIIYDFGGRRLNEIYPDKRLAQAALRAAKPGWFPVGAYGAGRSAVTGG
                     IFGCHARSGQGAAFRQIGDVKIAVFTVVNALGVVVDRDGKVVSCHKGSRWPDDLTASY
                     LMRNAPASLQPGWNGDEGAAPDRKNTTISLVVTNRKMSKAELNRLAVQVHTSMARAIQ
                     PFATEMDGDVLYAVSTAEVETGPGNFLPSAEIGVAASELMWDAVLAAVPPARQLPTVK
                     RAKPLTAAAAAAFAGDYRFSGLASLKVWFEDGALRAKATGERDVFAIKRDAAQELLPV
                     SSDTYMVPGRYPLVLQFRGGELVLNPGRWQQVGRRN"
     gene            17843..19282
                     /locus_tag="VN034_10780"
     CDS             17843..19282
                     /locus_tag="VN034_10780"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWV61131.1"
                     /translation="MRSSYAPPLTSCIDGEWLPRGNRTLRPVVNPSTGAVLGELPEVT
                     ADDLDRALDAADRGFRTWRGVPVDDRAAILPRAADILRTRIDQIATVIATEQGKPIVQ
                     ARAEAAAGIRFFDIHAEEAKRAYGRVLVRPSGQRATVTYEPVGPSVGFAPWNFPFFNV
                     ARKIAPALAAGCSIISKPAEETPASAILIQQALFEAGLPVDASQLVFGDPDGISRHLI
                     ASPTIRKISFTGSGPVGKHLMKLAADGMKRTTMELGGHAPVIVFDDADLNKVLSLLVP
                     GKFRNAGQVCVSPTRFYIQQGIYDQFARGFAERTAATIMTGNPLDPGITMGPLANSRR
                     PETVSTLIGDALRHGARTLHGGESLAGEGYFHQPTVLVDVPDEARIMQEEPFGPVAML
                     RPFATPDEAIEQANRLPFGLAAYVFTRNLGTAMRASDGIESGMVGVNTIGLSTGDAPF
                     GGVKESGHGLEDGPEGLKAFMVSKSIHMM"
     gene            complement(19371..20402)
                     /locus_tag="VN034_10785"
     CDS             complement(19371..20402)
                     /locus_tag="VN034_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWV61132.1"
                     /translation="MAELVRVAALTGYFPAMRGLGADPRPLLRECGLSPELFANPERL
                     ISARATMRLLERSAEVTGCETLGLRMAEERALANLGVASLLIAHQPTLRLALQAITEF
                     RNQINSTLVLHVEPFGEEVVLREDFALSSPEPHRQSSDLALGVLARLCASVLGDGWSP
                     LSVGFSRAKPPASEMQVYRRLFQCDVTFDCEFNGIVIAATDLDRPAERADSALAGHAR
                     ELISSVMNPAMRTASQEVEQMIMLLLPSGRATVQYCAESMATTVRTLQRMLDADGTSF
                     SELLNRARMQLSTQYLANPRTRITDVAEMLGYGSIGAYTRWHSQMFGISPRQWRASAL
                     QRQPESSVF"
     gene            20514..21491
                     /locus_tag="VN034_10790"
     CDS             20514..21491
                     /locus_tag="VN034_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWV61133.1"
                     /translation="MAKAVRFYEAGGPEVLRIEDVAVGEPGPGEVRIRHVAVGCNFAD
                     TYFRSGYYPAQLPAGIGVEGAGVIEAVGDGVTGFAPGDRVAYNGSPLGAYSEARIMPA
                     APLFKLPDVISFEDAAASTMRGLSATYWLAKTNPRMKAGDTILLHAAAGGVGLLAVQL
                     AKLMGLRVIGTVSSEAKAEKALSLGCDEIIFYRREDVAARVKELTDGEGVTTVFDSVG
                     QDTFEGSLKSLKRRGVFVACGTASGPFPPVDAFQLLMQGSVYFTRPGFADYYADPAER
                     AELSDLWFGHLAAGRVKVEIGQRYALDDCVAAHRELEAGQTIGSSIFVL"
     gene            21504..22352
                     /locus_tag="VN034_10795"
     CDS             21504..22352
                     /locus_tag="VN034_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWV61134.1"
                     /translation="MKIEKLTTHIGAEVRGVDLAEAARSDDLFGEIRAALLEHKVLFL
                     RDQDIARADHVAFASRFGALEDHPVVGSDPDHPGLVQIYKDETSPPDYYENSWHCDAT
                     WRDAPPLGAVLRCVACPPVGGDTMWANMAKAYADLPDPVKATIAPLKARHSIEASFGA
                     RMSEEQRHALKARFPDAEHPVVRTHPETGEKILFVNGFTTHFTNFHTAENVRYGQDYA
                     PGASQLLAWLIGRAAIPEYQVRWRWQPNSVAIWDNRSTQHYAVMDYPPTIRRMERAGI
                     VGDRPF"
     gene            22374..23438
                     /locus_tag="VN034_10800"
     CDS             22374..23438
                     /locus_tag="VN034_10800"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HWV61135.1"
                     /translation="MQFLDDSLLPETQDKLVITVAPYGPEWHPDDFPEDIPLSMEEHI
                     QKAVDCYNAGATVLHMHVREEDGTGSKRLSKFNELIAGVRARVPDMIIQVGGSISFAP
                     ESEEDGAEAKWLSDEVRHMLADLKPAPDQVTIAVNTTQMNIMDLMTDEEYGQTSLANP
                     ALYQAYREMVVPAGPQWVEEHLRRLTAAGIQPHFQLTCMPDMETVERLVRRGLYKGPL
                     NLTWVGIGGGFDGPNPNNFMEFIRRAPDGSTVTLESLMRHVLPVNVMAMCLGQHVRCG
                     IEDTLYDQHGNKMTSVQGIEQIVRIAKELGREVATAKEAREIYKIGVQYNSVEETLAK
                     LGMAPNRQPGQRALPLRYAA"
BASE COUNT         4405 a         7624 c         7333 g         4242 t
ORIGIN      
        1 atagccggca caattcaccg gactcacgat cgggctgccc tgcgtgatgg catcgatcag
       61 ataggtaccg gcgatataat agttgaggct gccggccgag ccgaggctgt gccggtagtc
      121 gaggcgatag tcgatgcccg acgcttccat cttcgcgatg ttcgcgttga gcagcggcac
      181 gtcgtaaagc tggccgtcgg gggtccggtt ccgcagcggc tgacagaaga tgttgctcag
      241 cgacatgtcg gcgttgcacg cggtgatgac cgactgcaaa cccccgccga acgagccgat
      301 cgcatcgtcg atcttgatgt tgtagtaatc gaccgtcagc tgcaggttcg gaatgaattc
      361 ggggcggatg accgcaccga ccgaccaggt gtccgacgtt tcttcctgca ggttcggatt
      421 gccgccggtg atcgcccgga tctgctggtt ttcctgctgg aacacatcga tcaccgctgc
      481 ggggatgccg agcgaattga cgcagaaatc gcgctcctgc tgcgtacggt cagcgcccgc
      541 gttacagaag tcgagcgcct gcggcgcggt gttggtcgcg cccgaataga gctcgacaac
      601 gttcggtgcg cgcaccgcgc gctgatacag gccccggaac ttcaggccgc ggaacggcga
      661 atattcgccg cccagcttgt acgaccagac cgaacccgcg gtcgaataat ccgaaaagcg
      721 gaggccgccc tcgaaagcca gcagttcgac gcccggcatg tcggcgagga tcggcacgta
      781 aagctcgcca tagagttcgg agacatcgaa cgagcccgcc gtcgggctga ggccggccga
      841 gcgcgacagc gccttgccct ggacatagag ctggtcggga tcgaacgacg agctttcctt
      901 gcgatattcg gcgccgatcg agatgccgac gtcacccgcg ggcagcgtga agagcgagcc
      961 cgtcaggctg gcgccgacct gcttctgccg gatcgtcgtg atattggtca gatcggtgta
     1021 gatatagtcg atcatttcgg gggtcagcga attgaacccg aaaggattga gcacgacgca
     1081 ctccggatcg gcgaccgcgc acttgtacgg atcgttggga tcggtcagga agccgttggc
     1141 gaaattgtcg atcagcacgc cgccgccctg aacgtcggtg accttggtct gaccctggtt
     1201 atagaagact tcccacttca accggtcggt cagattgccc ttgagcccga cgacgaagcg
     1261 gtagaagtcg cgcgtctggt cgctggtgcg cgagccgccg tcgagcagac gccggttgat
     1321 gttgacgggc accaggctgt tgccgtccgc atccatgttc gtgctcagga aatcgcgcaa
     1381 cgtcggatcg agatagggat tgcgaaggtc gagctggaag ccggggataa agcccgagct
     1441 gctcatcggc accgcgccga gcgtcgagtt gatcttgctg tggacgtacg acccttccca
     1501 gaaggccgtg acgtcgtcgg tcacgtcata atgaccgatc accgatccct gccagcgctg
     1561 catcggcatc tggatattgg tgaaggggcg atcgttgaag ccgtcgctcg gcttgtaggc
     1621 gcgccagccc tcgtcggcga agaagatgcc tgcggtgccg atggtattgc cataggggtc
     1681 ggtcagcccg acgagctgcg ggctgaagat cgtgccctgc ggcgtcacgc cgttgcccgc
     1741 cggcacgagg accggcttgc cgtcgacgat ggcatcggcg aaatattcga ccgcatggtc
     1801 gcgggcggcg cccttgacca tcgcgcgatc ataatagctg ccgtggaaca ccatattacc
     1861 gcgtccgtcg gcaaaattgc cgccgacggt cagctccaga ttgtaacgct ccgcgtcacc
     1921 ctcgtcgctg atgccatagg aaccgccgat ctcgaaaccc tcgaaatcat ccttggtcac
     1981 gaagttgacg acacccgcca tcgcgtccga accatagacg gccgacgcgc cgccggtcac
     2041 gacttcgacg cgcgcaacca gcgagggcgg aatcatgttg aggtcgacga cgccgtcctg
     2101 cgacgcaccg acgatccggc gcccgttctg cagaaccagc gtgcgcacgg tgccgaggtt
     2161 gcgaagattg accgtggccg aaccattgcc cgcattcgac tgcgcgcccg ccccggcaac
     2221 cgcctgcggc agcgcgttga gcaattgctc ggtttcgacg gtcgccgaat atttgatttc
     2281 gtcctgaccg atcacattga cgggactgtt ggattcgacg tcgggccgcg ggatgcgcga
     2341 cccggtgacg acaatagcgc cctcttccgc ggcaaccggc tcctgcgcca tcgccgaccc
     2401 catgaccgcc atcgacagga acagcgccga accgcccctc aatcctgccc gaacagctgc
     2461 tacccgcttc atacccatgc tcgtcactcc ctgaactgga acgcccgcct gcgaaattca
     2521 cggtcgctgg cgttcgccgc cagtcattcc cgtgaacagt tgcgtgattt tgatcatatt
     2581 ggcaccgcgc agtcaattcc gtgacgcaaa aaaatgtgaa aagtgtcatt ttagatgcgc
     2641 ggccgccttt ctcgccatgc atttcgtccg gcgcaggagc cccaaatatt ctagacaatg
     2701 tcttgttatt aaaggaataa tattcctgct cacgccaagg acggccccct ctcccggaag
     2761 cgacggattc ggagatcgag gcggcccttc cgcttcgtcc tgccgcttgg cggacgcaac
     2821 atggccactt gaccaagggc tccagctata acatcataac caaaatcacg cgtttttagt
     2881 tttaataatc aagcggtgga tcgatgattc gccggtgggc aacagaggag accctatcga
     2941 tgcgcaaggc attcgtcgtg gcgattaccg ccctgatcgc aagcaccacc agccttaccc
     3001 cgctcgcggc ggccggcgcg gcggagcagg tctcgaccgt gcaggccacg ccgccggacg
     3061 gcaagggcag ctatgacgag ctggttacgc tgttcacggc cttcaatgaa tggaagacgc
     3121 cgcgggcgat cgatggcgtc gtcgactata gccccgccgc cgtcgagaag cgccgcaccg
     3181 agctgcgcgc ctttcaggcc aggctgcccg acttcgcggt cgcgcgctgg gatcggcaca
     3241 agcaggtcga ctatctggcg gtccgcgcgc aaatggactt ggccgacttc acgctgaacg
     3301 tcaccaagcc ctgggcacgc gatccgggca tgtatgtcga cgagcttcag cgactggcct
     3361 ataccgatct gccgctggag ggagaggcgc tcaccacctt ccgcgcgcgg ctgcgtacga
     3421 ttcccgccta tctcgcgcag gcgcgcatca atctcgattc ggttgcggcc gactatgccg
     3481 atctcgcgat ccacagcctg tcgcatggcg acggcgtagg ccatggcatg ccctaccgcc
     3541 cggtcgaacc cgaaggcctg atcggctggt tcgccgacct gcacggtcgc gctgcgaaac
     3601 agcagcccgc gttgctgccg gatatcgagg ccgcggagca ggcgatcacc ggtttccgcg
     3661 actggctggt cgacgcgcgc ccgaccatga ccgtgtcggc cggggtcggc aagcccgcct
     3721 acgactggtt cctgaccaac gtccggctga tgccctatga ttccgaccag atcgtcacgc
     3781 tggctcagcg cgagctcgaa cggcactggg caaattatac gaccgagcgg caccgcaatc
     3841 gcaatctgcc cgaactcgag ctgccccgat cggccgagga atatgaagcg caactcgccg
     3901 gcaccgacgc caagatccgc aaatggttga aggatgagga gatcatcacg attcccgact
     3961 atattccggc cacctaccag gaagtcggct tcaacgcgcc atggatcgtc cgcccgcacg
     4021 ggccgatgtt ctgggagcag gtgcagttcc gcgacgcctc gcccgaccat cttcacgcaa
     4081 cctttcccgg ccaccggttc gacggcatga ccggcaagcg ggtttcgcac ccgatccgcc
     4141 gccatatcag tgacagcggc cggaccgaag gctggggctt ctacctagag gaaaacgcgc
     4201 ttcaactggg gctgttcgac gatcgtccgc gcacccgcga gctcatctat attttcggcc
     4261 tcttccgcgc cgcccggacg atcggggacg tcaagatgca gcgcaaccag atgaccgtcg
     4321 accagacgat gaaattctgg aaagaatgga cgccctatct cgatgacaat gtcgcccgcg
     4381 tcgatgcggg catctatatc cggcgtccgc cgggttatgg catgacctat acgatcggcg
     4441 cgatgcagct tcaaaagctg ctggtggacc gcaaacgcca gctcggcgac aaattttcga
     4501 tcagggatta tcacgactat ctgatgaaca ccggccgcct gccggtatcg ctactgcgct
     4561 atgacctcac cggctacgac gatgaaatca aaaagctatg gcgtcacgaa ccgttgacca
     4621 ccgacgtcag acacaaaccg tagcgaaccg atgagcagcg ggcgggcgag acacgcgatg
     4681 ccgagcgagg tcagcgcata attggcggga atagcggcga gcgtgcgtgc cgcattccag
     4741 cgctggcgcc gcggggccgg gaagccttgg tcgctatcgc cagaaccgcc gcgcgaggct
     4801 gatcagacat catggcaatc gccgaggcgg cgccgacacc gcaacatgca ggattggcaa
     4861 catctttcgc ggcgcccctt gtgcctaaaa gccgacgtgg ttctggcggc tcggaggcga
     4921 tagggtgcga aggtttgact ggcaggacgg tttgagcatt tccgatcggc tcgacgaaca
     4981 ggagcggatg attgccacgg ccgcccgcgc ctattgcagc gaccggttgc acccgcgcgt
     5041 cagggacgcc tatgcccgcg agcattgcga tccggacata ttccgcgaga tgggcgaaca
     5101 gggcctgctg ggcgtcaccg tgcccgaaga atatggcggc atcggcgctt cctatgtcgc
     5161 ctatggcctc gtcgcacgcg aagtcgaacg ggtggattcg ggctatcgtt cgatgctgtc
     5221 ggtccaatcc agcctcgtcg tccatcccat cctggcctat ggctccgacg cccagaagcg
     5281 acgctatctg cctgcgctcg ttgcgggcac cagtatcggc tgtttcggcc tgaccgaacc
     5341 cgatgcgggg tccgaccccg gcgccatgcg aaccagggcg acggcggtcg acggcggata
     5401 tcgcatcgac ggcaacaaga cctggatttc gaacgcgccg atcgccgacg tattcatcat
     5461 ctgggccaaa tcggaggcgc acggcggtgc catccggggc ttcatcctcg aaaaggggat
     5521 ggcggggctt tcggcaccga agatcgaggg caagctcagc ctgcgcgcgt cggttaccgg
     5581 gatgatcaac ctcgacggcg tgatcgttcc cgaagcggcg ctcttgccgc atgtcgaagg
     5641 gctgaagggc ccgttcggtt gcctcaaccg cgcgcgatac agcatcgcct ggggcgcgat
     5701 gggcgccgcc gaagcctgct atgcggccgc acggcaatat ggcatcgacc gtcagcaatt
     5761 cggtcgcccg ctcgccgcaa cgcagctttt ccagaagaag ctggccgaca tggccaccga
     5821 gattgcgctg ggcctgcagg ccgcattgca ggtgggccgc ctgatggacg aagacgccgt
     5881 tgcgcccgag atgatctcgc tcatcaagcg gaacaatgtc ggcaaggcgc tcgatatcgc
     5941 gcgcgccgcg cgcgacatgc acggcggcaa cggcatttcg ggcgaatatc cggtgatgcg
     6001 ccacgcgatc aatctggaaa cggtcaacac ctatgaaggc acgcacgaca ttcatgcgct
     6061 gatcatcggg cgcgcgatca ccggcctggc ggcgttctga catgtccagt cccgccagga
     6121 cgccgcttgc cgggctgaag gtcgtcgagc ttgcccgcat cctggccggg ccatgggcgg
     6181 gacagatcct cgccgatctg ggcgccgagg tgatcaaggt cgaaagtccc gatggcgacg
     6241 acacccgcaa atggggccca cccttcgtcg acaatggcga cggaacggcg gacgccgcct
     6301 atttccactg cgccaaccgc ggcaagacgg cgcgggcaat cgatttttcg tcgccggcgg
     6361 gcaagacgga gcttatcgaa ctgatccgcg acgccgacgt cctcatcgag aatttcaagg
     6421 tcggtacgct ggcgcgctac gggctcgatc atgccagtct tgccgcgatc aacccccggc
     6481 tcatctattg ctcgatcacc ggtttcgggc agaccggccc ctatgccggc cgcgcaggct
     6541 atgatttcgt cgtccagggg atgagcggga ttatggacct caccggaatg cccgatgccc
     6601 cgccccagaa gatcggcgtc gcctatgccg acatcatgac ggggctctat gcggtcatcg
     6661 cgatcgaggc ggcgctgatc cagcgcgcga cgacaggcac cggacagcat atcgacatgg
     6721 ccttgctcga cgtcatggtc ggaaccctcg ccaatcaggg gctgaactat ctggcgtcgg
     6781 gtatttcacc gtcgcggctg ggcaatgccc atcccaatat cgcgccctat gaagcctttc
     6841 gctgcgccga cggctgggtc atcctcgcgg tcggaaacga ccggcaattt gcgcgattgg
     6901 tcgatgcgct cgcgctcgac gagcgtccgg aatggcaaag caaccccggc cgtgtcgccg
     6961 atcgcgccgc actatccgcc gcgattacgg caaagctcga tcgatggaag agccgcgagt
     7021 tgctggcgat gatggacgaa ctcggcgtgc cggccggccc catcaatcgc gtcgaggatg
     7081 ccttcgccga cccccagatc cttcatcgcg agatgcgtct tgctctcgat cgcgaggacg
     7141 gcgccgccat accggggatc cgcacgccca tccgcttttc ggatgcctcg ctttcctgca
     7201 accgtccggc gccaaggctc ccgcgctcta tgtcctgatc acaagcgatg ccggcctgac
     7261 gaattcgcca gaatgccgac accgttgatc aggcccggac gcgttacctc gcctccacac
     7321 catattcctt cgcggtctca tcgagacttt tcgtcatctt gtcggccagc tcgtcgatct
     7381 cgccgcgcgt gatcgtcagc ggggggcaga gcaccatcgc atggccgacc gcgcgcgcga
     7441 tcaagccgct tcgcaaggcg atttcacggc aagtgaccgc cgccttttcg gacggatcga
     7501 agaataatcg ttcggtgcga tccttcatca gctggagccc cgcaagcaat ccggcgccgc
     7561 gcagttcgcc gaccagcgga tgaaagtcgg cgatttgttg cagcacggta cggaaatagg
     7621 ggccgacatc ctcacggacg cgatcgacca gcccctcgcg cgcgataatg tcgatattcg
     7681 cctccgccac cgcacaagcg accggatggc ccgaataggt atagccgtgc gggatggccc
     7741 cgccttcata gagtgtttta tggacgcgct cgccgatacc caccgcggcg atgggcagat
     7801 agcccgacga caatcccttg gccatcggca tcaggtccgg ctcgatcccg taatattgcg
     7861 aaccgaacca ttcgccgagg cggccgaagc cgcagatgac ttcgtcggcg acgagcagaa
     7921 tgtcgtgctt gcggcaaatc ttctggatgc gctcccaata gcccggcggc ggatcgatca
     7981 cgccgcccgc cccctgaacg ggttcggcga taaaggcggc gacattggcg gcaccgattg
     8041 cggcaatctt gtcttcgagc gcggcggccg ccacttcggc aaattgttcg ggcgtctggt
     8101 tgacgccgtg catgtaccag tgcggttcca tgatatgttc gaaaccgggg agcagttcat
     8161 gccccatgtc gtgcatggcc ttcattccgc cgaggctcgt cgcggccaat gtgctgccgt
     8221 gatagcccag attccgcccg atgaagacgc gcttcgatgg ctgcccgcac agtttccaat
     8281 aatgatagac aaggcggatg acggtgtcgt tcgcctcgga ccctgaattg gtgaagaaga
     8341 aattgtcgat gccttcgggc gtgacttcgg aaaggcgctt ggcgagcgaa atctgcgacg
     8401 gcgtcgcgct ctggaaaaag ctgttataat agggcagctc gcgcatctgg cgcgccgcga
     8461 cgtcgaccag ttcatcgcgg ccataaccga cgttgacgca ccagagcccc gccatgccgt
     8521 cgaggatctt gttgccgtcg ctgtcccaga catagctgcc gtcgccgcgc gtaatgaccg
     8581 tcgtgccgcg ctcggccagt tcgcgcggat cggaaaaggg gtgaaggtga tgccggtcgt
     8641 ccagctcgcg ccatctgtcg gtcgaaaatc cagcttccac gatcgatact ccctttgccg
     8701 tcggttcgag ggaggttttc tcggacttgg cgcgcgggag aaagatgagg aaagtgccgc
     8761 gggcgttgcc gtgaggacac cagcccgggc aacgcgcttc aatcgcggtc gagattttcc
     8821 atcagatccg cgatctgcag atcctcggcg gcgccgcggc cgcggagcgc ggcgatcacg
     8881 cgctgccggt cgcccggcgg gtgcgtctgg ctgagcttcg tcaccccgtc gatacgctcg
     8941 atcgcgaccc ggaacgaccg gatcatcggc agcagaaact cgaccttgcc ctccggcgca
     9001 tcctccattg tccagtcgct gccgttgtaa cgctccgaca tggcgatggt gcgccgcata
     9061 atcgcaagct gctcggcgtc atcgtcgatc ggggtcaatc gtccgcggac ttgcgcggtc
     9121 atatagttcc aggtcgggac cgtcggccgc tcgacgtacc aggacgagga aatataggca
     9181 tgcgggccgg caaagatcgc tagcgcggat tgcccgtcgc tcagcaccgc cgcgtgggga
     9241 ttggcccgcg ccagatgccc cacgagcact atctcgtcgg gggcgtcgct cgcgaaatag
     9301 atcgggacgc tcgtcgcgaa gggcgcgcca tcgctggccg ccgaaacaag ctgcgcaaac
     9361 gggtatcggg caaccagcgc gcgcggatcg ggagcccggt aatgttcggg gacgaacatc
     9421 cggtgccggg cttcttgctc ttccatatcg catcctcctg ctcccgccct taatccggcc
     9481 tgccgcgccg cctgcaagtt gccaaggcga aaaggggtgg tgttgcgtcg gcaccagccg
     9541 gatcggtgac cggggcacac cagtccccgt ccccttcgca ccgatcccgc ccgacgaacg
     9601 ggttatagtg cgatgcagcg cccccatttc aacaggacga gcgatgagca gccagccctc
     9661 cgatattgtc gatgcctatg ccggaaagct ttttccgggc gagcagttcg cgaccttttc
     9721 cgcgaccgaa aaaagctttc ccacccgcac cgtttcgcgg ggtacggggg atgtttcggc
     9781 gttgcgtcct gccgcgacga cattgcccga ccttccgatc gtgaccggca ccgaaagctg
     9841 cgacctgatc gattatgtgt cgcgcaaccg tctggtgggc ctgacgatcc tcaagggcgg
     9901 cgagatcgtc cacgaacgat atgagggcgg cctcaccccc gagatgcgct ggatttccat
     9961 gtcgatggcg aaatcggtcg ccacgacgct gatcggtgcc gcgatccacg acggatatat
    10021 cggcggggtc gacgatccgc tgaccgaata tctgccggtg ctgcgcggca gcggctatga
    10081 cggggtgtcg atccgtcacc tgatgcgcat gacatcgggg gtcgaatggg atgatacgca
    10141 caccgacccc cggtcgcatc gacgccatat gctgggcctc caggtcggcg gacagcatgg
    10201 cgcgatcatg gattatatgg cgacgctgcc gcggatcgcg gagccgggaa gcgtgtggaa
    10261 ttacagcacc ggggaaaccc acgtcgtcgg cgcgctggtc aaggccgcga ccggaaaatg
    10321 gcttgccgac tatctcagcg aaaaaatctg gtcgcgtgtc ggcatgcaat ccgacgccta
    10381 ttggtggctc gaagcgccgg acgggctcga ggttgcggga agcggccttt gcgcgacgat
    10441 gcgcgattat gtgcgtttcg ggctgttcgc gctgaatggc ggcgtcgccg gcggcgaacg
    10501 ggtggtgccc gagggctggt tcgccgaagc cggcggcgcg gagcatcgcg gcggcgatgc
    10561 gttcaactat ggctatatgt ggtggccggt gcccgaccgg agtggaagct tcggcgacgg
    10621 agcctacagc gcgcgcggca tcttcggcca atatatctat atcaacccgg cgcgcagcat
    10681 tctcgtcacc gtcctcagca gtcgcgccaa gccgaaattt tccgaagcca ttcccgacaa
    10741 tgattttttc aacgccgttg tcgacgcgat cggctgatca actgcgcccc ggcgcaaatt
    10801 cgggcagttc cttggcgatc gtctcgcgca gccagcgccg gaacgtccgg accaaacggt
    10861 cgtcccagcg atcgcggcgc gcgatcagca cataatgccc catccggaca gggaagctgt
    10921 ccttgcccgc cccgatgtcg atcaacgccc ccgattgcag atcgtcgcga atgatcaggc
    10981 tgttcgccaa cgaaataccg cggccatggc gcgcggcatc gacggtcaga tggccctgcc
    11041 acaaacgcgg accgccgagc cgttcttcgc agcccacgcc ataggatcgc agccattgca
    11101 cccaattgtt cgtgccgctt tcgtgcagaa gttcgtgcga aagcaggtcg gccggctcga
    11161 cgatcgcgtc gcgcgcctcc agatagcggc tgctcgcgac cgcgatgatc ggcacgtggg
    11221 caatcgttcc ctgccgctcg gcggccgagg gtgcggccac ctcctcatag gtcgcgttga
    11281 accggatatc gagatcggcc tcgtggcccg cgaaatcggg cgacgcgtcg gtcggtcgca
    11341 gctcgaagtc gacgcctgta ttgcgcgctt cgaacgctgc gaaatgctgc gagagccaat
    11401 gcagcgcgaa acccggcgtg caccagatcg acaggctgtt atgctgcccg ctgttgagca
    11461 catcgagcgt cgcatgggca atgtcttcga gcgcctggct gatcctcgcg tgatagatgc
    11521 gcccctgttc ggtcagcacg atcccgcccg gcgcacgcgc gaccagttga acgccaagcc
    11581 aggtttcgat cgaccgcaaa tggcggctca cgaccgcgtg atcgcggctg agccatgcgg
    11641 ccgcctttcg gatgcccccg aggcgcgcca ccgcatcaaa ggcccgcaat gcctcgaacg
    11701 gcgggagcga ttttcgctcg ggcggacgag gcggcgcgct catgtcgagg cggccgcaat
    11761 cgaaaagata gggtgcattc atgatatttc ccacaggcga tgttcgaggg tcaatcggaa
    11821 tttccgacac gcaaatcctc gatcccgcga gggagatgcg cgagatgcct aagcgccgat
    11881 cgatggtgcc ctgtgatcac caggcaaccg gcctttgtcg cctgttcgca ttttccgtac
    11941 cgcattctag tacggaccca gtggaagggg acagaaagtg acacgatgcg atcgcgacag
    12001 gagcctgcgg ctcccatgac gacagcttcc tccgctccgc tttatccagc gtccggcgcc
    12061 gccgacgacg gcaaagagat tttcggccac ccgcgcgggc tcgcctatat cgtcttcacc
    12121 gaaacctggg agcgcttctc cttttacgga atgcaggcgc tgctcattct ttatatgacg
    12181 ctgcatctct tcaagccgga gaatagcgcc gctgtcgccg gtttcgaggg ctatcgcgcc
    12241 atcatggcta cgatattcgg gccgctgagt acgcaggcgc tcgcggcgca gacgttcggc
    12301 ctctacgtcg gcttcgtcta tttcatgccc gtattcggcg gattgctcgg cgaccgctat
    12361 ctgggccgca aaaattcggt gatcatcggc gcggtcttca tggcggccgg gcatttcctg
    12421 atggcgttcg aggcgctttt cctgttcgcc ctgctcgcac tcgtgattgg ttcggggttt
    12481 ctgaaaggca atctcgcggc gcaggtgggc tatctctatg ccgccaacga caggcggcgc
    12541 gacgccggct tttccatcta tgtcttcggc atcaatgtcg gtgcgttcgt ggcgccgctg
    12601 atctgcggca cgctaggcga actctatgga tggcactatg gtttcggcgc cgccgggatc
    12661 ggaatgctga tcggccttgc gatctatctc aagggccgcc gctacctgcc gcccgagaga
    12721 atccggctcc agcaaggcga gcaggtccgc ctgcaacccg gcgacggaac gaaaatagcg
    12781 gccattgccg ccatgctcgc catcaccgcg ctctattgga cggcgcaatc gcaagtgtgg
    12841 aacagctatc cgctgtggat caaggatcag gtcgaccgca atctgggctt cgcgaccgtg
    12901 ccggtgacct ggttccagtc gctcgattcc ctggcggtgc tgctcctcgc ccccgccgtg
    12961 ctctggttct ggaaacgcca gtcgcggcgc gccgccgagc ccgcggacct tgccaagatc
    13021 ggcctcggct gcgcggtttt cgcgacggcc tgtctgctgc tctcggtcgg agagattctc
    13081 gcgggcagcg ggcaggtcgc aatcgtctgg ccggtcctct tccatttcgt ctgcgcgatc
    13141 ggcttccttt atcttggccc catcgcgctt gcgttgacgt cgcgcgccgc cccgcctgcc
    13201 gtcaatgcga tgatggtcgg ctgttactat ctggcgatat tcgccggcag cttcgcgagc
    13261 ggatggcttg gccgcttcta tgaaaccatg ccgccagcgg ctttctgggc gatgcacggt
    13321 gcgatcgtcg gcaccggcgc gctgttgatc ctggcgttcg gccggccgct gctgcgcgcg
    13381 atggccatcc agacacaagc ggacgaaggc gcgcaatgat ccggagcctt gcatcgatat
    13441 aagcccggcc tgtccccgca ggcatgtcca agccggctgc cagtcaggca ccggggcgct
    13501 tagagcgccg tgcgttaaat tggcatcgcc cccaattccg ttcgtgtcga gcgaagtcga
    13561 gacacccatc ggccaaagcg caagagcgac gggtgtctcg acttcgctcg acacgaacgg
    13621 gacggtggat cagattaaaa gctcgacgct ctacggcagc catcaacgac aaccaatgtc
    13681 gcgaaccttc ctgcacgcgg atcagcccgg cgatgatcgg ggctcgcata acgcacaaat
    13741 tgctgacgcg ctgaccttag ccgggggcgc aacggaaaac gccgcatccc gtcaaggaat
    13801 gcggcgaaaa tatcctgcga aagtctgcct cgcgaggggg atcagatcag aatttggccc
    13861 cgagcttcag cccgatcacc cgcgggcggg tccggttgga gttcgcccag ccctgatagg
    13921 gatcgatcgc gcgattgatg ttgagcaggt tcgacccata gatctcggcg ttgaagcgcc
    13981 cgaaatcgaa cccgagacgg gcgttgagca acgacagcgt atccgatttc tgcggcagga
    14041 cgttcgcaaa gaagctcgaa cggtcggtat aggtcacctt gtcgcggaag ttgaaatcga
    14101 cccggacaaa agcatcgaca ctatccgaca cggcaaagtc ataattggcg ctggccgaat
    14161 agctgagctt gggcgtgtag tcggtcaggt cgccaatgat gttcacctga tcgccgggat
    14221 cggtgtcgac gatcttgctg tcgagatagg cggcgttgac gccaaaagtc agccccgttg
    14281 ccgggcgcac ctgtacgcca aattccagcc ccttgacccg cgccttgccg atattgctgg
    14341 attcgggaag gagcagcccg tcgacattga ccagccgccg ccggatcatg tccgaatagt
    14401 tggtgataaa tccggccacc tcatactgga aaagaccgcc gccaaagctg cccttgctgc
    14461 cgacttcata ggtttcgaca ctttcagggc catagggacc gaacggatcc tcgttgaagc
    14521 cgccgctgcg gaaaccctgc ccatagctcg catagaggcg ggcgttgtcg gcaaagcgat
    14581 agctgacaac gacccgggga tcggtcgatt caaacgatgc ccgtttggta acgcccgcgc
    14641 ccggcacccg ttcgctcagg tcgcgctgct tgtcgcggaa ataacgaagg ccgccgccga
    14701 tggtcagccg atcggtcagg tcataggaga gatcggcgaa tatcgaataa ttctcggtct
    14761 tccctttcga gaaaaaatag aggttgtcga tgaggacccc gccattttcg tagaggtcgc
    14821 cattttccgc gaacacataa ccataatcgg cactcccgcg gtcctttccc agcgcataaa
    14881 agccgccgac cgtccattgg aaaggtcccg aagccgacgc caggcgaagc tcctggctga
    14941 actggctgac gtggccatag cgtgcgtcgc tgccttcgac aaaaccgaac gagaaattcc
    15001 ccggccgcgg aatgtaggtg aagggatatt gctcgtcgaa ttcggtatag gtcgtcgcgc
    15061 tcaccagctg ggcgaaatcg aggtcgtagt tgagctccag attataggtg gtgaaatcga
    15121 acttcttggg aatcagaacc gtcgcccggt cgatcccgat atcgaccgtc cggtcgggct
    15181 cctcaaaacc cagcccgagc cgcgtctccg cccggtggat ctggaccatg gccttggccg
    15241 aaaaacggtc ggtgggctgg agcagcaatt gcgcccgcac attgagcaat tcggtgccgt
    15301 tgccgtcctt gattccggct tcgggctggt cgatccagcc cccgccgtct tcgtatagcg
    15361 ccgcaacgcg cagcgccgcc ttgccggtgg cgatcggaac gttgacgacg gccgacgtct
    15421 tgtacccgac gtcgccttga tcgacgtcat agacctcgcc ggtcacagcc gccgagaagg
    15481 catcgagatc gggcttgttc gtgatgtagc ggatgacgcc cgccgcggcg ccctgcccgt
    15541 aaagcgttcc ctgcggccct ttgagcacct cgatgcgttc gagatcgagc gcgacggggc
    15601 tgagctgggt gaagccgccc agcgagagcg gcgcctcgtc cagatattgt ccaaccagcg
    15661 cgcccgagcc cgactgattg gcgagccccc gcatgaagat cgtgtagctg ccgggaccat
    15721 cctcacgcat ggtcaggccg ggaaccgaaa aggacaggtc ctgaacgctc gccacgcctt
    15781 tttccgccag ctcctcgccg ccgagcaccg aaatcgccat cgggacgtcg acgagctttt
    15841 gctcgcgctt ctgcgcggtg acgacgatca tctcgtcatc gccttccgcg ccgccgcctt
    15901 gggcaaaagc cggctgagcc aaggcggcgg cgagcagtgc aacggccgag cctgtgtaga
    15961 atgtcgcttt catctgtccc tcccagaacg gtcctatctg tcgatggcaa gctatccgat
    16021 gaccggcgca ggccatgatg cgaaatcgcc gagccatggt gcgcaaaagt caccggagcg
    16081 gctcgggcga cgccgcggag gcggccgccc ataggcgccg atccgatggc aatccgtcgc
    16141 ttcccggatc gcgcaagcac aagcaccgcc gggtgaaccc gggcggacga tgcgcgtcag
    16201 ttcctgcggc ccacctgttg ccagcgcccc ggattgagga ccagctcgcc gccacgaaac
    16261 tgcaaaacca gcggatagcg gccgggcacc atataggtat cggacgatac cgggagcagt
    16321 tcctgggccg catcgcgctt gatcgcaaac acgtcgcgtt cgccggtggc ctttgcccgc
    16381 agggctccat cctcgaacca gaccttcagg ctggcgagtc cgctgaagcg atagtcgccg
    16441 gcaaaggccg cagccgccgc ggcggttaac ggcttcgcgc gcttgaccgt cggcaactgc
    16501 cgcgccggcg gaacggctgc cagcacggcg tcccacatca attccgatgc cgcgacgccg
    16561 atttcggccg aaggcagaaa attgccgggc ccagtttcga cttcggcggt cgaaacggcg
    16621 taaaggacat cgccgtccat ctcggtggca aagggctgaa tcgcgcgtgc catcgaggtg
    16681 tgaacctgta ccgccagccg gttcagttcg gccttggaca tcttgcggtt ggtcacgacg
    16741 aggctgatcg tcgtattctt gcgatcgggc gcagccccct cgtcgccgtt ccagccgggc
    16801 tggaggctgg caggcgcatt gcgcatcaga tagctggcgg tcagatcgtc cggccaacgc
    16861 gaccccttgt gacacgagac gaccttgcca tcgcgatcga cgacgacgcc cagggcgttg
    16921 acgacggtga atacggcgat cttgacgtcg ccgatctgcc ggaaggccgc tccctgtccc
    16981 gaacgggcat ggcaaccaaa aatcccgccg gtcaccgccg agcgcccggc gccataggca
    17041 ccgaccggaa accatccggg cttggccgcg cgcaatgcgg cctgcgccag ccttttgtcg
    17101 ggatagatct cgttcaatcg gcgcccgccg aaatcataga tgatcgaccc ttcgaccagt
    17161 ccgacattat cccaatgacc gccgcgcgcg ccgtcgtcct tcagcgcggt gttcaccgcc
    17221 gtgaccgctt ccagcccata ccatgatccg cccgcgagca cgatcgcgtc gagttccgga
    17281 aactcgtacc ccagccggag ataatccgaa ttgacggtcc cgggtccgcc gccgcgcgta
    17341 tcgatcgcgg catgcacccg ctccttgaaa cggatcacgg tcagcccggt cgggccgtct
    17401 tcatattgcc cggtgccgat cgacagcatc ggccagtcga agctcagtgc gtccttggtt
    17461 cccgccccgt cgttgagcgt catgggcagc gccgcctggc tttcggtagc cgctggcgga
    17521 gcctcggctc cccatgcgac acccgtaaag gtcgcactca gggccagtat ggcggccaat
    17581 gccgtgctac gggattgcgc gcggattcct gtcattctgc tcaccttcgg acatcgggga
    17641 tctggcgccg agctttcaca acaaatcgcc aaaagttaag tgcaaagaat tggccggccg
    17701 gtgcgctgag ggcaccgatg atattgctga acgcgactgg gggctggcca tgtgccccct
    17761 agaatcgccg catgacccat cctcccttgt tcgtccgccg atgccctgca ttcggccttt
    17821 ctcttgcaac aggtcccttc cgatgaggtc ctcctacgct cccccgctga catcgtgcat
    17881 cgacggcgaa tggctgccca ggggcaaccg gaccctccgg cccgtcgtca atccatccac
    17941 cggcgccgtg ctgggagaat tgcccgaagt cacggccgac gatctcgacc gggcgctgga
    18001 tgccgcggac cgcggttttc gcacctggcg cggggtccct gtcgacgacc gcgccgcgat
    18061 cctccccagg gcagcggaca tcctccgcac ccggatcgac cagatcgcga ccgtgattgc
    18121 gaccgaacag gggaagccga tcgtccaggc acgcgccgaa gccgcggccg gtatccgttt
    18181 tttcgatatc catgccgagg aggccaagcg ggcctatggc cgcgtcctcg ttcgcccgtc
    18241 gggtcaacgc gcgaccgtca cctatgaacc cgtcggcccg tccgtcggct ttgccccgtg
    18301 gaactttccc ttcttcaacg tcgcacgaaa aatcgccccc gcgctcgccg ccggttgctc
    18361 gatcatttca aaacccgccg aagaaacccc cgcatcggca atcctgatcc agcaagcgct
    18421 gttcgaggcg gggttgcccg tcgatgcgag ccagctggta ttcggcgacc ccgacggcat
    18481 cagccgccac ctgatcgcct cgccgacgat ccggaaaatc agcttcaccg gatcgggacc
    18541 cgtcgggaaa catctgatga aactcgccgc cgatggcatg aaacgcacga cgatggagct
    18601 cggcggccat gcgcccgtga tcgtgttcga cgatgccgat ctgaataagg tgctgtcgtt
    18661 gctggtaccc gggaaattcc gcaacgcggg gcaagtctgc gtatcgccga cgcgcttcta
    18721 catccagcaa ggcatatacg accaatttgc ccggggcttt gccgaacgga ccgcggcaac
    18781 gatcatgacg ggcaacccgc tcgaccccgg catcacgatg gggccgctcg ccaatagccg
    18841 ccgccccgag accgtcagca cgctgatcgg cgacgcgcta cgccatggcg cgcggacgct
    18901 ccatgggggc gagagcctcg ccggcgaggg gtatttccat cagccgaccg tattggtcga
    18961 tgtccccgac gaagcgcgga tcatgcaaga ggaacccttc ggcccggtcg ccatgctgcg
    19021 ccccttcgcg acgcccgatg aggcgatcga gcaggccaac cgcctgccct tcggcctcgc
    19081 cgcctatgtg ttcacccgaa acctcggcac cgcgatgcgt gcgtcggacg ggattgagtc
    19141 gggtatggtc ggggtgaaca cgatcggcct gtcgacgggc gacgcgccat tcggcggcgt
    19201 caaggagtcg ggacacggac tggaagacgg gcccgaaggc ctgaaggctt tcatggtttc
    19261 aaaatcgatc catatgatgt gagacaacag aagcgagccg cgcattcggc gtcaaaatac
    19321 tgaaccaggc acagggccga tggctgccgg tcggcttttg gggtgtcagt tcaaaagacg
    19381 gacgattcag gttgccgctg gagcgccgaa gcgcgccatt ggcgaggcga tataccgaac
    19441 atctggctgt gccagcgggt gtaagcaccg attgatccat agccgagcat ctcggcgaca
    19501 tcggtgatcc gcgtacgcgg attggcgaga tattgcgtcg aaagctgcat tcttgcgcga
    19561 ttgagaagtt cgctgaagct cgttccgtcg gcgtcgagca tccgctgcaa tgtgcgcacg
    19621 gtggtcgcca tcgattcggc gcaatattgg actgtcgcgc gccccgaagg cagcaggagc
    19681 atgatcatct gctcgacctc ctgcgaagcc gtgcgcatcg cgggattcat caccgacgag
    19741 atcagctcgc gcgcgtggcc ggcaagcgcg gaatcggcac gttccgcagg ccggtcgaga
    19801 tcggtggccg cgatgacgat accgttgaac tcgcagtcga atgtgacatc gcactggaac
    19861 agccgccgat agacctgcat ttcggatgct ggcggcttgg cgcgcgagaa tcccaccgaa
    19921 agcggcgacc aaccatcgcc cagaacgctc gcgcagaggc gcgcgagaac gcccagcgcg
    19981 agatcggacg actggcgatg cggctcgggg ctgctcagcg caaaatcttc gcgtagcacg
    20041 acctcctctc cgaagggttc gacatgcaat acgagcgtcg agttgatctg gttacgaaat
    20101 tcggtgatcg cctgaagcgc caggcgaagc gtcggctggt gcgcgatcaa caggctggca
    20161 acgccgagat tggcaagggc acgctcctcg gccatccgaa ggccgagcgt ctcgcacccg
    20221 gtaacttcgg cgctgcgctc cagaaggcgc atcgtggctc gcgccgagat caatcgttcc
    20281 gggttcgcga acagttcggg cgacagcccg cattcgcgca gcaaaggccg cgggtcggca
    20341 cccagccccc gcatcgccgg aaaatagccg gtcagcgctg cgacgcggac gagctcggcc
    20401 acgcttctct cctctgatcg cgcccgttgg cgtctaatgc caaaattatg tcacgaaatg
    20461 tgaaaagtgc aagcgggcgc tgctctaccc ccatcgtaat gggagagtga gagatggcca
    20521 aagccgttcg tttttatgaa gccggtggac ccgaagtgct ccggatcgag gatgtcgcgg
    20581 tcggcgaacc cggtccgggc gaggtccgca tccgccatgt tgcggtcggc tgcaattttg
    20641 ccgacaccta tttccgctcg ggctattatc ccgcgcagct tcccgccggg attggcgttg
    20701 aaggcgcagg cgtgatcgag gccgtcggcg atggcgtcac cggtttcgcg cccggcgacc
    20761 gcgtcgccta taacggcagc ccgctcggcg cctatagcga ggcgcgcatc atgcccgccg
    20821 cgccgctgtt caagctgccc gatgtcattt cgttcgagga tgccgccgcc tcaaccatgc
    20881 gtggtctgtc ggcgacctat tggctggcca agaccaatcc gcggatgaag gccggcgaca
    20941 ccattctgct ccacgccgcc gcaggcgggg tcggactgct cgcggtacag ctcgccaagc
    21001 tgatggggct acgcgtcatc ggcaccgtgt cgagcgaggc gaaagccgaa aaggcgctct
    21061 cgctcggctg cgatgagatc atcttctatc gccgcgagga tgttgccgcg cgggtcaagg
    21121 aattgaccga tggcgagggc gtgacgacgg tgttcgacag cgtcggccag gacacgttcg
    21181 aaggctcgct caagtcgctg aaacggcgcg gcgtgttcgt cgcctgcggc accgcgtcgg
    21241 gacccttccc gccggtcgac gccttccagc ttttgatgca ggggtcggtc tatttcaccc
    21301 gccccggctt cgccgactat tatgccgacc cggccgagcg cgccgagctg tcggacctgt
    21361 ggttcggcca tctggccgcg ggccgcgtca aggtcgaaat cggccagcgc tacgcccttg
    21421 acgactgcgt cgccgcgcat cgcgaactcg aagccggcca gacgatagga tcatccatct
    21481 tcgtgctctg agggagaggg acgatgaaga tcgaaaagct caccacgcat atcggtgccg
    21541 aagtccgcgg cgtcgacctt gccgaagcgg cacgcagcga cgacctgttt ggcgaaatcc
    21601 gcgccgcgct gctcgaacac aaggtcctgt tcctgcgaga tcaggacatc gcccgcgccg
    21661 atcatgttgc cttcgcgagc cgcttcggcg cgctcgagga tcatccggtg gtcggaagcg
    21721 accccgatca tcccggcctc gtccagatct acaaggacga gacgtcaccg cccgattatt
    21781 atgagaatag ctggcattgc gacgcgacct ggcgcgacgc gccgccgctg ggcgcggtgc
    21841 tgcgctgcgt cgcctgtccg cccgtcggcg gcgacacgat gtgggcgaat atggcgaagg
    21901 cctatgctga cctgcccgac cctgtgaagg cgaccatcgc gcccttgaag gcgcgtcatt
    21961 cgatcgaggc gagctttggc gcgcggatga gcgaggaaca gcgccacgcg ctgaaagcac
    22021 gcttccccga tgccgagcat ccggtcgtcc gcacgcaccc tgagacgggc gagaaaatcc
    22081 tgttcgtcaa cggcttcacg acgcacttca ccaatttcca caccgccgaa aacgtccgct
    22141 acggtcagga ttatgcgccg ggcgcgagcc agctcctcgc ctggctgatc gggcgcgccg
    22201 cgatccccga atatcaggtg cgctggcgct ggcagccgaa cagcgtcgcg atctgggata
    22261 accggtcgac ccagcattat gccgtcatgg attacccgcc cacgatccgc cgcatggaac
    22321 gcgcgggcat cgtcggtgat cgccctttct aataatcgat aaggagagag aagatgcagt
    22381 ttctcgacga cagcctgctt cccgaaacgc aggacaaatt ggtcattacc gtcgcgccct
    22441 acggccccga atggcacccc gacgatttcc ccgaggacat tccgctctcg atggaagagc
    22501 atatccagaa ggcggtcgac tgctacaacg ccggcgccac ggtgctccac atgcatgtcc
    22561 gcgaggagga tggaacgggt tcgaagcgcc tgtcgaaatt caacgaactg atcgccggag
    22621 tgcgtgcgcg cgtccccgac atgatcatcc aggtcggcgg ctcgatctct ttcgcgccgg
    22681 aaagtgagga ggatggcgcc gaggcgaaat ggctgtccga cgaggtccgc cacatgctcg
    22741 ccgacctgaa gcccgcgccc gatcaggtga cgatcgcggt caacacgacc cagatgaaca
    22801 tcatggatct gatgaccgac gaggaatatg gccagacctc gctcgccaat ccggcgctgt
    22861 atcaggcgta ccgcgagatg gtggtgcccg cggggccgca gtgggtcgag gagcacctcc
    22921 gtcggctgac cgcggcgggc atccagccgc atttccagct cacctgcatg cccgacatgg
    22981 aaacggtcga gcggctcgtg cgccgcgggc tctacaaggg tccgctcaac ctgacctggg
    23041 tcggcatcgg cggcggcttc gacgggccca atccgaacaa cttcatggaa tttatccgcc
    23101 gcgcgcccga cgggtcgacc gtcacgctcg aatcgctgat gcggcatgtg ttgccagtca
    23161 acgttatggc gatgtgcctc ggccaacatg tccgctgcgg catcgaggac acgctttacg
    23221 accagcacgg caacaagatg acctcggtgc aggggatcga gcagatcgtc cgcatcgcga
    23281 aggaactggg ccgcgaggtt gcgaccgcga aggaagcgcg cgagatctac aagatcggcg
    23341 tacaatataa ttcggtcgag gaaacgctcg ccaagctcgg catggcgccc aaccgccagc
    23401 ccggccagcg cgcactgccg ctgcgctacg cggcctgagg cgggaatgcg atgatgacct
    23461 tcggcggcga caccacgatc ctcttcgttc ccgggcttcg ggatcatgtc ggggatcatt
    23521 ggcagaccca tgccgcgcgg gcgatccccg gcgcggtgac ggtcgcgccg ctcgccgccg
    23581 acaggctgag ccgcgcgggg caag
//
LOCUS       DATMSU010000401         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_111001,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000401 DATMSU010000000
VERSION     DATMSU010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111001"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1083
                     /gene="recA"
                     /locus_tag="VN034_10805"
     CDS             4..1083
                     /gene="recA"
                     /locus_tag="VN034_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWV61136.1"
                     /translation="MAGQLSLVESGKSVNNTDRQKALDAALAQIDRAFGKGSVMKLGS
                     KEAMQVEAISTGSLGLDIALGVGGLPRGRVIEIYGPESSGKTTLALHTLAEAQKTGGT
                     VAFVDAEHALDPVYARKLGVNIDELIVSQPDTGEQALEIVDTLVRSNAIDVLVVDSVA
                     ALVPRAEIEGEMGDSHVGLQARLMSQSLRKLTGSISRSRCMVIFINQLRMKIGVMYGN
                     PETTTGGNALKFYASVRLDIRRTGQIKNGDEIVGNTTRVKVVKNKVAPPFKQVEFDIM
                     YGQGISKIGEILDIGVKAGLVEKSGAWFSYDSIRIGQGRENAKNFLTENPELKERLEA
                     AIRGRTDEVAEEMMAGPDDEGDDDN"
     gene            1256..2089
                     /gene="speB"
                     /locus_tag="VN034_10810"
     CDS             1256..2089
                     /gene="speB"
                     /locus_tag="VN034_10810"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HWV61137.1"
                     /translation="MPAIRLFGLPTDINSSFERGAAAGPAAIRAMLWSDRGNMASELG
                     CEIGTDIALTDDGDLPLTENTAQDDAMIRRHIAYVREDGEVPLALGGDHAVTFPLVEA
                     AAACHGPVTILHFDAHPDLYDDFAGNPRSHASPFARICEAGHAKRLVQIGIRTLTNHC
                     REQAARFGVEIVPMPDFSPELVPVLDGPLYISIDLDGLDPSAAPGVAHPEPGGLTVRE
                     LLAVLYKQTAPIVGADIVELHPGRDIGGVTAILAAKLVRELAALIDRNGPINPDPKES
                     P"
     gene            2086..2712
                     /locus_tag="VN034_10815"
     CDS             2086..2712
                     /locus_tag="VN034_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWV61138.1"
                     /translation="MSLIVHHLNNSRSQRILWLLEEIGAAYEIKYYDRDRVTNLAPPE
                     LLAVHPLGKSPVIEDDGRVITESGAITEYLCERQGGGHLVPERGTDAYISHLEWLHFA
                     EGSAMTPILLRIYTARLGDAAAPLEPRIAQQLDAHFAYMESRVGENGHFIGDELSAAD
                     IMLSFPAEIAIMQGMAPRYPKLAAFVNACHDRPAWQRAREKGGAYYGY"
BASE COUNT          517 a          968 c          852 g          435 t
ORIGIN      
        1 caaatggccg gacaattgtc actcgtcgaa tcggggaaat cagtgaacaa cacggacagg
       61 cagaaggcgc tcgacgccgc gctcgcgcag atcgatcgcg cgttcggcaa gggctcggtg
      121 atgaaattgg gctcgaagga agcgatgcag gtcgaggcga tctcgaccgg ctcgctcggc
      181 ctcgacatcg cgctcggcgt cggcggactg ccgcgcggcc gcgtcatcga aatctacggt
      241 cccgaaagct cgggcaagac gacgctcgcg ctgcacacgc tcgccgaagc gcaaaagacc
      301 ggcggcaccg tcgccttcgt cgatgccgaa catgcgctcg acccggtcta tgcccgcaag
      361 ctcggggtca acatcgacga actcatcgtg tcgcagcccg acaccggcga acaggcgctc
      421 gagatcgtcg acacgctcgt ccgctcgaac gcgatcgacg tgctcgtcgt cgactcggtc
      481 gccgcgctcg tcccgcgcgc cgagatcgag ggcgagatgg gcgacagcca tgtcggcctg
      541 caggcgcgtc tgatgtcgca gtcgctgcgc aagctcaccg gctcgatcag ccgctcgcgc
      601 tgcatggtga tcttcatcaa ccagctgcgc atgaagatcg gcgtgatgta cggcaacccc
      661 gaaacgacga ccggcggcaa tgcgctcaaa ttctacgctt cggtccgcct cgacatccgc
      721 cgcaccggcc agatcaagaa tggcgacgag atcgtcggca acacgacccg cgtcaaagtc
      781 gtcaagaaca aggtcgcgcc gccgttcaag caggtcgaat tcgacatcat gtacgggcag
      841 ggcatttcca agatcggcga aatcctcgac atcggcgtca aggccgggct ggtcgagaaa
      901 tcgggcgcgt ggttcagcta tgactcgatc cgcatcgggc agggccgcga aaatgcgaag
      961 aatttcctga ccgaaaatcc cgaactcaag gaacggctcg aagccgcgat ccgcggccgc
     1021 accgacgaag tggccgagga aatgatggcc ggccccgacg acgagggcga cgacgacaat
     1081 tgatccgata gccatccggc cggttcgcct tgctcgaacc ggccggccga cggcggcggg
     1141 ccaccagccc ctttgacctg cgctcccctc tcccaagcag gtcggcccgc ccgccgttga
     1201 tcctcgcgcc gccgttgctt cttgcgccgc tcttgccacg agctacaagc ggcgcatgcc
     1261 cgcgatccgc ctcttcggcc tgcccaccga catcaacagc agcttcgaac gcggcgccgc
     1321 cgccggcccc gccgcgatcc gcgcgatgct gtggagcgac cgcggcaata tggcgagcga
     1381 gctcggctgc gaaatcggga ccgacatcgc cctcaccgac gacggcgacc ttccgctcac
     1441 cgaaaacacg gcccaggacg acgcaatgat ccgccgccac atcgcctatg tgcgcgaaga
     1501 tggagaagtt ccgctcgcgc tcggcggcga ccacgccgtc acctttccgc tcgtcgaggc
     1561 ggcggcagcg tgccacgggc cggtgacgat cctccacttc gacgcgcacc ccgatctcta
     1621 cgacgatttc gccggcaatc cgcgcagcca tgcctcgccc ttcgcacgca tctgcgaggc
     1681 gggccatgcg aagcggctgg tgcagatcgg catccgcacc ctcaccaatc attgccgtga
     1741 acaggcggcg cgtttcggcg tcgaaatcgt cccgatgccc gatttctcgc ccgagctcgt
     1801 cccggtgctc gacggcccgc tttatatctc gatcgatctc gacggcctcg acccgtcggc
     1861 cgcgcccggc gtcgcgcatc ccgaaccggg cgggctgacg gtccgtgaac tgctcgcggt
     1921 gctgtacaag cagacagcgc cgattgtcgg ggcggacatc gtcgaactcc atcccggccg
     1981 cgacatcggc ggcgtcaccg cgatcctcgc cgcgaagctc gtccgtgaac ttgcggcgct
     2041 gatcgaccgc aacggcccta tcaaccctga tccaaaggaa agcccatgag cctgatcgtc
     2101 caccatctga acaacagccg ctcgcagcgc atcctttggc tgctcgaaga aatcggcgcg
     2161 gcatatgaaa tcaaatatta cgaccgcgat cgcgtgacca atctcgcccc gcccgaactg
     2221 ctcgcggtgc atccgctagg caagtcgccg gtgatagaag atgacggccg cgtgatcacc
     2281 gaatcgggtg cgatcaccga atatctgtgc gagcggcaag ggggcgggca tctggtcccg
     2341 gagcgcggca ccgacgctta catcagccac ctcgaatggc tgcatttcgc cgaaggctcg
     2401 gcgatgacgc cgattctgct gcgtatctac accgcgcggc tcggcgacgc cgcggcgccg
     2461 ctcgagccgc gcatcgcgca gcagctcgac gcgcacttcg cctatatgga aagccgcgtc
     2521 ggcgagaatg gtcatttcat cggcgacgag ctgtcggccg ccgacatcat gttgagtttc
     2581 ccagccgaga tcgcgatcat gcagggcatg gcgccgcgct atcccaagct cgccgccttc
     2641 gtgaacgcgt gccacgaccg gccggcgtgg cagcgtgcac gcgaaaaggg cggcgcctat
     2701 tatggctatt gaccgtcgcg gccgcgcgtg gctcgccagc gcggtgttga tggccgcgat
     2761 tccggccgcg tc
//
LOCUS       DATMSU010000402         6044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_167621,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000402 DATMSU010000000
VERSION     DATMSU010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167621"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VN034_10820"
     CDS             <1..876
                     /locus_tag="VN034_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV61139.1"
                     /translation="AAAPAATSQPAPTAGSSAINGHVDVAGAAIHYQVHGDLASGKRP
                     LLVLHGSFMSGDAMMPLIAPFTASRPVIAIDARGQGRSGGVDGELSYERMADDAAAVL
                     GELGVKKADVLGYSMGGTLAIALAVRHPDKIGKQVILSGTSRLDGWYPEVLTVIGKLT
                     PETFAGSPIESEYKRLSPNPKKFATMIEKVKSLETKPWSWSDEDVRRIPGKTMVIIGD
                     ADGVTLNHAIHLFQLRGGGDVGAAIQGFLTAPPRARLAILPAMSHIGLMGEATTIAAM
                     VTPFLDDKPPPPPSF"
     gene            931..1905
                     /locus_tag="VN034_10825"
     CDS             931..1905
                     /locus_tag="VN034_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV61140.1"
                     /translation="MPYLPSPRSFGRRAPGSALVLALLATAAALAAAPAPLYAQTPAP
                     AAAAAAIKTGHADVDGARIYYQVHGDLGSGRTPLLVLHGAFMSGDAMAPFFAPLAATR
                     PVIAIDARGHGRTGDAPGGLSYDQMANDAAGVLAALKVEKADVLGYSMGAATALRLAM
                     LHPERTGKQVIISGAYRLDGMPPELIKSIAKLTPEIFAGSPMESEYKKQSPTPDAFPT
                     LVAEIKALDATDFAWPEDQIRAIKGKTMVISGDNDGTTLEHTIALFKLRGGYDLPVVL
                     QGFMTEAPKARLAILPASSHIGIMGEAATIAAMVTPFLDDKAPPPPSF"
     gene            1936..2607
                     /locus_tag="VN034_10830"
     CDS             1936..2607
                     /locus_tag="VN034_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV61141.1"
                     /translation="MPVNPDSNIEVTAFDWVPDFARGYVRDLRPRWACEEIGLDYAEH
                     LISAVNRPADHFLFQPWGQVPVLNDNGVRLFESGAILLHLAEKDERLMPRDAQARANT
                     LAWLFAAYNSVEPMLFELGNVDIFASDEEWAKLRRPSLIEFIQGRLGRLNDAMGDKQY
                     LVDEFTVADIAMATVLREGVEAGLIAEQPRLQAYLDRCLARPAFQRAMDAQLAAFSEE
                     KGPGA"
     gene            2765..3097
                     /locus_tag="VN034_10835"
                     /pseudo
     CDS             2765..3097
                     /locus_tag="VN034_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478908.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
     gene            3131..3484
                     /locus_tag="VN034_10840"
     CDS             3131..3484
                     /locus_tag="VN034_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HWV61142.1"
                     /translation="MGYIDGYLVPVPDGNKDAYRALAEKASQVFRDHGATRVVEAWGD
                     DVPDGEVTDYNRAAHKKDDESVVFSWVEWPSKEARAAGWEKVMADERMKNEGENSPFD
                     GKRMIYGGFAPIVEA"
     gene            3647..4285
                     /locus_tag="VN034_10845"
     CDS             3647..4285
                     /locus_tag="VN034_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV61143.1"
                     /translation="MIKLYGTPPTRALRAIWLLNELDLAHEVIAIDLGAGEQLTPEFL
                     ALNPAAKLPVLVDGDLVISESAAIQLYLADKYGDRFPGGGLIPETAEDRARMYHWLFF
                     LMTEIEAPLWRVALHSFLYAEDEKSEDEIALAKRDGRRMVAVLEQHMQGRDFIVGDAV
                     TVADFNAAFTLDWANEAGLLDEAPALRAWLEAMYARSKAPVTIAAAMAELEA"
     gene            4282..4833
                     /locus_tag="VN034_10850"
     CDS             4282..4833
                     /locus_tag="VN034_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61144.1"
                     /translation="MTRDAGDMKRIAACSCGAVTLELAGAPIVAATCYCHSCQTAGKA
                     FEAAPGAPGVVGADGGTPYVLMRKDRLEWLSGSDQLDEHRLTPDSPTRRFVARCCNSP
                     VALEFTKGHWLSIYSGRIAEGERPSAEMRTIVGDRPEGVELPDDIPNYKTPSGKFMWR
                     LLKAWAAMGFRAPPVEATKGFAR"
     gene            4830..5129
                     /locus_tag="VN034_10855"
     CDS             4830..5129
                     /locus_tag="VN034_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61145.1"
                     /translation="MSKMVERVDMLSATSPGHVVRVEKAKYDAMKDALLAAVPTGDPG
                     LTVAEIKARVLPLLPEHLFPGGAKAGWWLKGVQLDLEARGLIARENVKPLRLHRL"
     gene            5152..5868
                     /locus_tag="VN034_10860"
     CDS             5152..5868
                     /locus_tag="VN034_10860"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HWV61146.1"
                     /translation="MEHLSFPGESPEYRAARAGLLADEIALRRQIEAVAAKRRALPPG
                     GVVPEDYSFEWIGAYQRPERVTLSALFAGKPSIILQSFMFGPERDMPCDGCTHMLDAI
                     DGSARHVGQRAPLYVVAKSPIARLEAWAKARRWPHLTFLSDVDGRYSADYFGDTSKLT
                     DAKRAEHGLKPGENWDETIFNVFHKGGGTIRHVWASEMAYAPEDPGQHHRAGDLVDPL
                     WAFLDMTPEGRGDWFPSLTY"
BASE COUNT         1090 a         1996 c         2014 g          944 t
ORIGIN      
        1 gcggccgccc ccgcggcgac atcgcagccc gcccccacgg ccggcagcag cgccataaac
       61 ggtcatgtcg acgtcgcggg cgccgcgatc cactatcagg tgcatggcga cctcgcgagc
      121 ggcaagcgcc cgctgctcgt cctccacgga tcgttcatgt cgggcgacgc aatgatgccg
      181 ctgatcgcgc ccttcaccgc ctcgcgcccg gtgatcgcga tcgacgcccg cggccagggc
      241 cgctcgggcg gcgtggacgg cgaactcagc tatgaacgca tggccgacga tgccgccgcg
      301 gtgctgggcg aactcggcgt caaaaaggcc gacgtcctcg gctattcgat gggcggcacg
      361 ctggcgatcg cgctcgcggt ccgccacccc gacaaaatcg gcaagcaggt gatattgtcg
      421 ggcacctcgc ggctcgacgg ctggtatccc gaggtgctga ccgtcatcgg caagctgacc
      481 ccggagacct ttgccggcag cccgatcgag agcgaatata agcgcctgtc gcccaacccg
      541 aaaaagttcg cgacgatgat cgagaaggtg aagtcgctcg agacgaaacc ctggagctgg
      601 tccgacgagg acgtccggcg cattcccggc aagacgatgg tgatcatcgg tgacgccgac
      661 ggcgtcacgc tcaaccatgc gatccacctg ttccagctgc gcggcggcgg tgacgtcggc
      721 gcggcaatac agggttttct gaccgcaccg ccgcgcgcgc ggctcgcgat cctgcccgcg
      781 atgtcgcaca tcggcctcat gggcgaagcc acgacgatcg ccgcgatggt gacgcccttt
      841 ctcgacgaca agccgccacc gccaccatca ttctagcgcg ccttcgccgg aatgacgaac
      901 agacgatatt tgaacgagag gattgcccga atgccctatc tcccctcccc gcgctccttc
      961 ggccgccgcg cgcccggatc ggcgcttgtt ctggccctgc ttgccacggc cgccgcgctg
     1021 gccgcggcac cagcgccgct ctatgcccag acgcccgctc cggcggcggc agcggccgcc
     1081 atcaagaccg gccacgccga tgtcgacggc gcgcgtatct attatcaggt gcatggcgac
     1141 ctcggctcgg gccgcacgcc attgctcgtg ctgcacggcg ccttcatgtc gggcgacgcg
     1201 atggcgccgt tcttcgcccc cctcgccgcg acgcggccgg tcatcgcgat cgatgcgcgc
     1261 ggccacggcc gcaccggcga cgccccgggc ggtctcagct atgaccagat ggcaaatgac
     1321 gcggccggcg tcctcgcggc gctgaaggtc gaaaaggccg atgtgctcgg ctattcgatg
     1381 ggcgcggcga ccgcgctgcg cctggcgatg ctccaccccg aacggaccgg caagcaggtg
     1441 atcatttcgg gcgcctaccg gctcgacggc atgccgcccg aactgatcaa aagcatcgcc
     1501 aagctgaccc ccgaaatctt cgccgggtcg ccgatggaga gcgagtacaa gaagcagtcg
     1561 ccgacccccg acgcctttcc gacgctggtc gccgaaatca aggcgctcga cgccaccgac
     1621 ttcgcatggc ccgaagacca gattcgcgcg atcaagggca agacgatggt catatcgggc
     1681 gacaatgacg gcacgacgct ggaacatacg atcgcgctgt tcaaattgcg cggcgggtac
     1741 gaccttccgg tcgtcctgca gggcttcatg accgaagcgc ccaaggcgcg gctcgcgatc
     1801 ctgcccgcga gctcgcacat cggaatcatg ggcgaagccg cgacgatcgc cgcgatggtc
     1861 acgccctttc tcgacgacaa ggcaccgccg ccgccgtcct tttgatcccc ccaagaaaag
     1921 aagaggagtt tcgccatgcc cgtgaacccc gacagcaaca tcgaagtcac cgctttcgac
     1981 tgggtgcccg acttcgcacg cggctatgtc cgtgacctgc gcccgcgctg ggcgtgcgag
     2041 gagatcggcc tcgattatgc cgagcatctg atcagcgcgg tcaaccgccc ggccgaccat
     2101 ttcctgttcc agccgtgggg ccaggtgccg gtgctgaacg acaatggggt gcgcctgttc
     2161 gaaagcggcg cgatcctgct ccacctcgcc gagaaggacg aacggctgat gccgcgcgac
     2221 gcgcaggcgc gcgccaacac gctcgcctgg ctgttcgccg cctataacag cgtcgagccg
     2281 atgctgttcg agctgggcaa tgtcgacatc ttcgccagcg acgaggagtg ggcgaagctg
     2341 cgccgtccga gcctgatcga attcatccag ggccggctcg gccgcctgaa cgacgcgatg
     2401 ggcgacaagc aatatctggt cgacgaattc accgtcgccg acatcgcgat ggcgacggtg
     2461 ctgcgcgaag gcgtcgaagc cgggctgatc gccgagcagc cgcggttgca ggcctatttg
     2521 gaccggtgtc ttgcacggcc cgccttccag cgtgcgatgg atgcgcagct tgccgcgttc
     2581 agcgaggaga aggggccggg tgcctgacaa cagttcagcc cctcctcttc aggggagggg
     2641 cagcgagact tgggagcttg ctccctagtc gcagcggggt ggggccatcg gcatcgcgca
     2701 aggccgcgag cccccacccc gacccctccc ctgaagggga ggggctagat caaggagaga
     2761 gaagatgacc tatgtagaag gatttgtggt cgcggtgccg accgccaaca aggaagccta
     2821 tcgcaagcac gccgccgacg cggcgcatct gttccgggaa ttcggcgcga gccgtatggt
     2881 cgaggcgtgg ggcgacgatg ttcccgacgg caaggtcaac gacttcaagg gcgccgtgca
     2941 ggcgaaggcg gacgagaccg tcgtgttcag ctggttcgaa tatcccgaca aggcgacgcg
     3001 cgacgccgcg aacgaaaaga tcatgagcga tcccaggatg caggcgatgg gcgacaccat
     3061 gccgttcgac gccaagcgca tgatctatgc cggtttcgat tcgatcgtcg acgacgccgc
     3121 cgacggcaag atgggctata tcgacggcta tctggttccc gtccccgacg gcaacaagga
     3181 cgcctatcgc gcgctcgccg aaaaagcctc gcaagtgttc cgcgaccatg gcgcgacgcg
     3241 cgtcgtcgag gcgtggggcg acgacgtccc cgatggcgaa gtcaccgatt acaaccgggc
     3301 cgcgcacaaa aaggacgacg agagcgtcgt gttcagctgg gtcgaatggc cgagcaagga
     3361 agcgcgcgcc gcgggctggg aaaaggtcat ggccgacgag cggatgaaga atgagggcga
     3421 gaattcgccc ttcgacggca agcgcatgat ctatggcggt ttcgcgccga tcgtcgaggc
     3481 gtagcgctct actcctcccc cgtgccggag gcatggggag gtggcagccc gaagggctga
     3541 cggaggggct tgtgacgcac cgtcgcggcc cctccaccac cgcttcgcgg cggtccccct
     3601 ccccatcgct tcgcgacagg gaggatcgag atgaaagacc gaagagatga tcaaacttta
     3661 tggcactccc cccacccgcg cgctccgcgc gatatggctc ctcaatgaac tcgatctcgc
     3721 gcacgaggtg atcgcgatcg acctcggcgc cggcgagcag ctgacgcccg aatttctcgc
     3781 gctcaatccg gccgcgaagc taccggtgct ggtcgatggc gatctggtga tcagcgaatc
     3841 ggcggcgatc cagctctacc tcgccgacaa atatggcgac cgctttcccg gcggcgggct
     3901 gatccccgag acggccgagg atcgcgcgcg gatgtaccac tggctgttct tcctgatgac
     3961 cgagatcgag gcgccgctgt ggcgggtcgc cctgcacagc ttcctctatg ccgaggacga
     4021 gaaatcggaa gacgagatcg cgctggcgaa gcgcgacggc cggcggatgg tcgcggtgct
     4081 cgagcagcat atgcaggggc gcgatttcat cgtcggtgac gcggtcaccg tggccgattt
     4141 caacgccgcc ttcacgctcg actgggcgaa tgaggcaggg ctgctcgacg aagcacccgc
     4201 gctccgcgcc tggctggaag cgatgtatgc gcgctcaaag gcaccggtga cgatcgccgc
     4261 ggcgatggcc gagctcgaag catgacccgc gatgcaggcg acatgaagcg catcgccgcc
     4321 tgttcatgcg gcgcggtgac gctggaactc gccggcgcgc cgatcgtcgc ggcgacctgt
     4381 tactgccata gctgccagac cgccgggaag gcgttcgaag cggcgcccgg tgcgcccggc
     4441 gtcgtcgggg ccgatggcgg caccccctat gtgctgatgc gcaaggaccg gctggaatgg
     4501 ctgtcggggt ccgaccagct cgacgagcat cggctgaccc ccgattcgcc gacgcggcgc
     4561 ttcgtcgcgc ggtgctgcaa ttcgccggtc gcgctcgaat tcaccaaggg gcactggctg
     4621 agcatctatt cgggacgcat agccgaaggc gaacgcccgt ccgccgagat gcggacgatc
     4681 gtgggcgacc ggccggaggg cgtcgaactg cccgacgaca ttcccaacta caagacgccg
     4741 tcgggcaagt tcatgtggcg cttgctgaag gcgtgggccg cgatgggttt tcgcgcgccg
     4801 ccggtcgaag cgacgaaggg atttgcgcga tgagcaagat ggtggagcgc gtcgacatgc
     4861 tgagcgccac gagcccaggc catgtcgtgc gtgtcgaaaa ggcgaaatat gacgcgatga
     4921 aggatgcgct gctcgccgcc gtgccgacgg gtgaccccgg gctgaccgtc gccgagatca
     4981 aggcgcgggt tttgccgttg ctgccggagc atctctttcc gggcggcgca aaggccggct
     5041 ggtggctgaa gggcgtccag ctcgatcttg aggcgcgtgg gctgatcgcg cgcgaaaatg
     5101 tgaagccgtt gcggctgcac cggctataat gaaagcagaa ggaatcggga tatggaacat
     5161 ctgtccttcc ccggcgaaag ccccgaatat cgtgccgcac gcgccgggct gctcgccgac
     5221 gagatcgcgc tccgccgcca gatcgaggcg gtcgcggcga agcgccgcgc gctgccgccc
     5281 ggcggcgtgg tgcccgagga ttacagcttc gaatggatcg gcgcgtacca gcggcccgag
     5341 cgcgtgacgc tgtcggcgct gttcgcgggc aagccgagca tcattctgca aagcttcatg
     5401 ttcggtcccg aacgcgacat gccgtgcgac ggctgtaccc atatgctcga cgcgatcgac
     5461 ggtagcgcgc gccatgtcgg acagcgcgcg ccgctgtatg ttgtcgccaa gtcgccgatc
     5521 gcgcggctgg aagcgtgggc aaaggcgcgg cgctggccgc acctgacctt tctgtccgac
     5581 gtcgatggcc gctattcggc cgattatttc ggcgacacca gcaagctgac cgacgccaag
     5641 cgcgccgaac atggcctgaa acccggcgag aactgggacg agacgatctt caatgtcttc
     5701 cataagggcg gcggaacgat ccgccacgtc tgggcaagcg agatggccta tgcgcccgag
     5761 gatcccggcc agcaccatcg cgccggcgat ctggtcgatc ccttgtgggc ctttctcgac
     5821 atgacgcccg aggggcgcgg cgactggttc cccagcctga cctactgaca ccggaaaaaa
     5881 tcgaggagag agacgatggc cgaaacttcg aacttcatct ggtacgaact gatgaccccc
     5941 gatcccgcgg gcgcggcgcg cttctatggc gcggtggtcg gctggacgat agccgcgacg
     6001 cgcgatcccg cggcgggcga cgtcgattat cgcatgatcg gccg
//
LOCUS       DATMSU010000403         1842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_167631,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000403 DATMSU010000000
VERSION     DATMSU010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167631"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VN034_10865"
     CDS             complement(<1..157)
                     /locus_tag="VN034_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HWV61147.1"
                     /translation="MAEKIIVIDEGTTSTRTMLFAADGTPLGSAQREIRQHYPGPGLV
                     EHDAAEIW"
     gene            603..>1842
                     /locus_tag="VN034_10870"
     CDS             603..>1842
                     /locus_tag="VN034_10870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWV61148.1"
                     /translation="MTTFNDFGLHADINRALAAKDYTQPTPIQTQAIPPLMKGRDLCG
                     IAQTGTGKTAGFSLPSIHHLLTYPHRPTPRGCRMLVLAPTRELAAQIAQSCRDYGRFT
                     KLSVTTVFGGVPINKQIRDLSSGVDILVATPGRLLDLIDQRALRLDDVEIFVLDEADQ
                     MMDMGFIHSLRRIAKLLPSRRQNLFFSATMPKEIAHLAEQFLEDPVTVSVTPAATTVE
                     KISQYSTFVEQKEKQALLHSIVASEDIDRALIFTRTKHGADRVVRHLAGAGIKAMAIH
                     GNKSQSQRTHALQAFRSGDIKLLVATDIAARGIDVSGVSHVINFEIPNVPEQYVHRIG
                     RTARAGAEGKAISFIAPDERPYMRDIERVTRSKSEPMPLPDNFNELVRNLPKPAQPPR
                     DTGSKGRNPQRQREDAHRQRD"
BASE COUNT          350 a          629 c          548 g          315 t
ORIGIN      
        1 cccagatttc agcggcgtca tgctcgacga gaccggggcc gggataatgc tgccgtatct
       61 cgcgctgcgc gctgccgagc ggggtgccgt cggccgcgaa gagcatcgtg cgggtcgagg
      121 tcgtgccctc gtcgatgacg atgatctttt cggccaatgc acgtccccca aatctttgat
      181 gtcgagcctg ccaaagactg tggggcagga caagaagttc acgcggaggc gcggagacgc
      241 ggagaaaaga atattcgcgc aaaggcgcag agagtgtctc cgcgtgaacc aaaatctctg
      301 cgatctccgc gcctctgcgc gaatctcttt cgtatccagc gcatgacctc tgcgcacgcg
      361 gcgcaataat cttgcaattc agcgcaaccc atgccatatg attgtgcagc gcagcatggc
      421 gggccttccc gccgcaacca ccggcagcgt gattttcccg tccaaatcag gcgcgggacg
      481 agccggacca gcgctttgtc cccagaggca ccctttcacg cgggtcgttt cgaacccgcg
      541 gccgtgcgcc gtccgttcga accgaacgga tgcgcctgcg cgtcgctcta tgtgagtatt
      601 ttatgacgac tttcaacgac tttggcttgc acgccgacat caatcgcgcg cttgccgcca
      661 aggattatac ccagccgacc ccgatccaga cgcaggcgat cccgccgctg atgaagggcc
      721 gtgacctgtg cggtatcgcg cagacgggca ccggcaagac cgccggtttc tcgctgccct
      781 cgatccacca tcttttgacc tatccgcacc gcccgacccc gcgtggttgc cggatgctcg
      841 tgctggcgcc gacgcgcgaa cttgccgcgc agatcgcgca gagctgccgc gactatggcc
      901 gctttaccaa gctcagcgtg acgacggttt tcggcggcgt gccgatcaac aagcagatcc
      961 gtgacctgtc gagcggcgtc gatatcctcg tcgccacgcc cggtcgcctg cttgacctga
     1021 tcgaccagcg cgcgctgcgc ctcgacgatg tcgaaatctt cgtcctcgac gaagccgacc
     1081 agatgatgga catgggtttc atccactcgc tgcgccgcat cgccaagctg ctgccgtcgc
     1141 gccgccagaa cctcttcttc tcggcgacga tgccgaagga aatcgcgcat ctcgccgaac
     1201 agttcctcga ggacccggtg acggtgtcgg tgacgccggc ggcgacgacg gtcgaaaaga
     1261 tcagccaata ttcgaccttc gtcgaacaga aggaaaagca ggcgctgctc cattcgatcg
     1321 tcgccagcga agatatcgat cgcgccctga tcttcacccg caccaagcat ggcgccgacc
     1381 gcgtcgtgcg tcatctcgcg ggcgccggga tcaaggcgat ggcgatccat ggcaacaaga
     1441 gccagtcgca gcgcacccac gcgctgcagg cgttccgttc gggcgacatc aagctgctcg
     1501 ttgcgaccga catcgccgcg cgcggcatcg acgtgtcggg cgtcagccat gtgatcaact
     1561 tcgagatacc gaacgtgccc gagcaatatg tccaccgcat cggccgcacc gcgcgcgccg
     1621 gcgccgaggg caaggcgatc agcttcatcg cgcccgatga gcgtccgtac atgcgcgata
     1681 tcgagcgcgt cacccggtcc aagtcggagc cgatgccgct gcccgataat ttcaacgaac
     1741 tcgtccgtaa cctgccgaag ccggcgcagc cgccgcgcga caccggcagc aagggccgca
     1801 acccgcagcg ccagcgcgaa gacgcacacc gccagcgtga cg
//
LOCUS       DATMSU010000404         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_92480, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000404 DATMSU010000000
VERSION     DATMSU010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92480"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..446)
                     /locus_tag="VN034_10875"
     CDS             complement(21..446)
                     /locus_tag="VN034_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWV61149.1"
                     /translation="MRAMIIKSFGGADMLHWGDLPTPEPTVGEVLVRVVCAGVNPADW
                     KTREGKLSAYIQYHFPFVLGFDLAGIVEAVGEGVDAFSPGDRVFGMSGRGATGAMPNI
                     ASPTRCFLRRCRKDGASRRRRRSRLRGRRPMAGWPMSAR"
     gene            complement(457..1590)
                     /locus_tag="VN034_10880"
     CDS             complement(457..1590)
                     /locus_tag="VN034_10880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWV61150.1"
                     /translation="MKFSIIYEAQMVDTSRENEARCFHEIIEQAVLAEEMGFDTVWAV
                     EHTALTQYAHMSAPETFLAFLAGKTTRLGIGHGVVCLPPAMNHPVKVAERIATLDILS
                     NGRVHFGMGKGGTQQEAGTFGYDLGELQPMIDESMYLIPKIMVEDEIEHDGTHIRIPR
                     RPIHPKPYQDPHPPLYMACTRADALTTAGARGIGALVLGFAGPDDIASKNAIYRAAWA
                     NRKAEDQVGFRPTEHLAALCPALVLDDREKARKIGLRGQRFFVESLGYWYQGGPKPTV
                     EDLSGDEQAAILQQEKAAVVAYLSEEKITIGSEHTGAYEEVQDAYGTAEDCIRYVQRL
                     FDAGADEILFLFQMGAVPHEAILETIRNIGTHVIPHFRAQAAK"
     gene            1731..2495
                     /locus_tag="VN034_10885"
     CDS             1731..2495
                     /locus_tag="VN034_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61151.1"
                     /translation="MGMFDGKVALVTGGASGIGVAAALRFAEEGAAVVVADINEAGAE
                     AIAEKVRAAGGRARAIHCDVSSAADNDAMVAAAKQDFGRLDAAFLNAGAYAQTGFDES
                     DATTLDSMIAINLKGAWLGLQAVLPALESGGAAVVTASTSGMIGLNVAAAYSAAKHGV
                     IGAVRSASQSFARRGLRVNAICPGPVATAILGVNESAPIQPADALALPGADGVLLPQH
                     IAEVALFLASPRSGGINGQSLIADAGFLSSYPPMPE"
     gene            complement(2509..2958)
                     /locus_tag="VN034_10890"
     CDS             complement(2509..2958)
                     /locus_tag="VN034_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWV61152.1"
                     /translation="MTEAVNLAAIEEIRLLKARYLRALDTKDWPLLRSLLTDDMVGDF
                     REMPGGDPDEQLLTTGADAFVAGVTAALEGATTVHHVHSFEIRFTGARDAEGIWAMDD
                     HFWAGEGSDLAGRSRHGFGHYHDCYRKARGRWLIAKTRLSRIRVERD"
     gene            complement(2962..4125)
                     /locus_tag="VN034_10895"
     CDS             complement(2962..4125)
                     /locus_tag="VN034_10895"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWV61153.1"
                     /translation="MVGQVPDRATLVARACEQTGLSDFGDTWFFANIDALIPALNDEA
                     RLSEAGVGSAAAMIVNGLVNRLRHVDLVKRHPEILGETVEVAAVVVGLPRTGSTMLHR
                     MLASAPGMTGTRWFETQNYAPFPGEERGNPEPRREAARGFLDYMVATIPDLMSIHPMS
                     IDQPDEELIILGQLYSSTMIEGTYHVPGYARWLTENDRIQCYRDLRQILQSLQWQDPS
                     RKGAKWVLKTPGHLLALDAVIEVFPEAKIVMTHRDPVATVPSYCSMEHSLYRMVSDDV
                     DRESVARFWVPRLAELLDRFMAVRGTVAGRNFVDVRYADLLSDPIGEGTRVLEAAGIA
                     VTGDVRAGMAEWIEANKREDRAPHKYALEDFGLVRSDVEQRFAAYRAAFLEGK"
     gene            complement(4231..4401)
                     /locus_tag="VN034_10900"
     CDS             complement(4231..4401)
                     /locus_tag="VN034_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWV61154.1"
                     /translation="MALAGGGSLVEAADRLGIAHNTARSHLRSIFAKTGARRQSQLVH
                     LLHAGMPQRGGS"
BASE COUNT          732 a         1490 c         1414 g          773 t
ORIGIN      
        1 gagcacgctc tgccccgcct tcaacgcgcc gacatcggcc agcccgccat gggccgtcgt
       61 ccccgcaacc gggagcgccg ccgcctgcgc gaagctccat ccttgcggca acggcgcaag
      121 aaacaccgcg tcggcgacgc aatattcggc atagctcccg tcgcgcccct gcccgacatg
      181 ccgaacaccc ggtcgccggg gctgaaggcg tcgactccct cgcccaccgc ttcgacgatg
      241 ccggcaagat cgaaaccgag cacgaacggg aaatgatatt ggatataggc cgacagcttt
      301 ccctcgcgcg tcttccagtc ggcggggttg accccggcgc atacgacccg caccagcacc
      361 tcgcccaccg tcggctcggg ggtcggcaga tcgccccagt gcagcatgtc cgcaccgccg
      421 aaactcttga tgatcatcgc acgcatggtg ccctccttat ttcgccgcct gcgcgcggaa
      481 atgcgggatc acatgcgttc cgatgttgcg gatcgtttcg aggatcgcct cgtgcggcac
      541 cgcgcccatc tggaagagga agaggatttc gtcggcgccc gcgtcgaaca gccgctggac
      601 gtaacggatg caatcctcgg cggtgccata ggcatcctgc acctcttcat aggcgccggt
      661 atgctcggaa ccgatcgtga tcttttcctc ggagagatag gcgacgaccg ccgccttttc
      721 ctgctgcagg atcgccgcct gctcgtcgcc ggacagatcc tcgacggtcg gcttcgggcc
      781 gccctgatac cagtaaccga gcgattcgac gaagaagcgc tggccgcgca ggccgatctt
      841 gcgcgccttc tcgcggtcgt cgagcacgag cgcgggacag agcgcggcaa gatgttcggt
      901 cgggcggaaa ccgacctgat cctcagcctt gcggttcgcc cacgccgcgc gatagatcgc
      961 attcttgctc gcgatatcgt cgggccccgc gaagcccagc acgagcgcgc cgatgccgcg
     1021 cgcccccgcg gtcgtcagcg cgtcggcgcg cgtgcacgcc atatagagcg gcggatgcgg
     1081 gtcctgataa ggcttgggat ggatcgggcg gcgcgggatg cggatatgcg tgccgtcatg
     1141 ctcgatctcg tcctcgacca tgatcttcgg gatcagatac atgctctcgt cgatcatcgg
     1201 ctggagttcg ccgaggtcgt atccgaaggt gcccgcctcc tgctgagtgc cgcccttgcc
     1261 catgccgaaa tgaacgcggc cgttcgacaa tatatcgagc gtcgcgatgc gctcggcgac
     1321 cttgacggga tggttcattg cgggcggcag acagacgacg ccgtgcccga tgccgagccg
     1381 cgtcgtcttg cccgcgagaa acgcgaggaa ggtctcgggc gccgacatat gcgcatattg
     1441 ggtgagcgcg gtgtgctcga ccgcccacac ggtgtcgaat cccatttcct cggcgagcac
     1501 cgcctgctcg ataatctcgt ggaagcagcg cgcctcgttt tcgcgcgagg tgtccaccat
     1561 ctgcgcttcg taaatgatcg agaatttcat ggcccgcgct cctctccgcc atctgctttt
     1621 cggcgtgcat agggcggcgg aaaggccccg ttcctcgtcc aattggatca attcgcgccg
     1681 cgccgccgat gctaggcaag gtgcgacatt gggcaggaag aggaattgcg atgggcatgt
     1741 tcgacggcaa ggtcgcgctg gtgaccggcg gggcttcggg gatcggcgtt gcggcggcgc
     1801 ttcgcttcgc cgaagagggc gccgcggtcg tcgtcgccga tatcaacgaa gccggtgccg
     1861 aagcgattgc cgagaaggtt cgcgccgccg gcggccgcgc gcgcgcgatc cactgcgacg
     1921 tcagcagcgc ggcggacaat gacgcgatgg tcgccgccgc aaaacaggat ttcggccggc
     1981 tcgacgccgc tttcctcaac gccggggcct atgcgcaaac gggtttcgac gaatccgacg
     2041 cgacgacact cgacagcatg atcgcgatca acctcaaggg cgcctggctc ggattgcagg
     2101 cggttttgcc cgcgctcgaa agcggcggcg cggcggtggt caccgcctcg acctcgggaa
     2161 tgatcggcct caatgtcgcc gccgcctaca gcgccgccaa acatggggtg atcggcgcgg
     2221 tgcgctcggc ctcacagagc ttcgcgcggc gcgggctgcg cgtcaacgcg atctgccccg
     2281 ggccggtcgc gaccgcgatc ctgggggtga acgaatcggc gccgatccag cccgccgacg
     2341 cgctcgcgct tcccggagcc gatggcgtgc tgctgccgca gcatatcgcc gaggttgcgc
     2401 tgttcctcgc cagcccgcgt tcgggcggga tcaacggaca gtcgctgatc gccgatgccg
     2461 gttttctttc cagctatccc ccgatgcccg aatgagcgtt gggcggcgtc agtcgcgctc
     2521 gacgcggatg cggctgagcc gcgtcttcgc gatcagccag cgcccgcgcg ccttgcggta
     2581 gcagtcatga taatgcccga agccatggcg cgagcggccg gcaagatcgc ttccctcgcc
     2641 cgcccagaaa tggtcgtcca tcgcccagat gccctcggcg tcgcgcgcgc cggtgaagcg
     2701 gatctcgaag ctgtggacat gatggacggt cgtggcgcct tcgagtgccg cggtgacccc
     2761 cgccacaaag gcgtcggcgc cggtggtcag caattgctcg tcgggatcgc cgcccggcat
     2821 ttcgcggaag tcgccgacca tgtcgtcggt cagcaggctg cgcagcagcg gccagtcctt
     2881 ggtgtcgagc gcgcgcagat agcgcgcctt gagcagcctt atctcctcga tcgcggcgag
     2941 gttgacggct tcggtcatga tctatttccc ctccaggaag gcggctcgat aggccgcgaa
     3001 acgctgttcg acatcggagc ggaccagacc gaaatcttca agcgcatatt tatgcggcgc
     3061 gcgatcctcg cgcttgttgg cttcgatcca ctcggccatg cccgcgcgca cgtcgccggt
     3121 gaccgcgatc cccgcggctt cgagcacgcg cgttccctcc ccgatcggat cgctcaagag
     3181 gtcggcatag cggacgtcga cgaagttccg ccccgcgacc gttccgcgca ccgccatgaa
     3241 acggtcgagc agttcggcaa ggcgcggcac ccagaagcgt gcgacgcttt cgcggtccac
     3301 atcgtccgag accatccggt agaggctatg ctccatgctg caatagctcg gtaccgtcgc
     3361 caccggatcg cggtgcgtca tcacgatctt cgcctcgggg aatacctcga tcaccgcgtc
     3421 gagcgcgagc aggtggccgg gggtcttgag cacccatttg gcgcccttgc ggctcgggtc
     3481 ctgccattgc agcgactgaa ggatctgcct gaggtcgcgg tagcattgaa tgcggtcgtt
     3541 ctcggtcagc cagcgcgcat agcccggcac atgataggtg ccctcgatca tcgtgctcga
     3601 atagagctgg ccgaggatga tcagctcctc gtcgggctga tcgatgctca tcggatggat
     3661 cgacatcagg tcggggatcg tcgcgaccat atagtcgagg aagccgcgcg ccgcctcgcg
     3721 ccgcggttcg ggattgcccc gctcctcccc cggaaaagga gcgtaattct gcgtctcgaa
     3781 ccagcgcgtg ccggtcatcc caggcgccga ggcgagcatg cggtggagca tcgtgctgcc
     3841 cgtgcgcggc agcccgacga ccaccgcggc gacctcgacc gtctcgccca ggatttcggg
     3901 atgacgcttg accagatcga cgtggcgcag ccggttgacg aggccattga cgatcatcgc
     3961 cgccgcgctg ccgacgcccg cttcgctgag gcgcgcctca tcgttcagcg cggggatcag
     4021 cgcgtcgata ttggcgaaaa accaggtgtc gccgaaatcc gaaagccccg tctgctcgca
     4081 ggcacgcgcc accagcgtcg cgcgatccgg cacttgaccc accatctctc ccatcccgta
     4141 caccttccgc attgcgtaga taatttgtct attcaaatac gatattcgta tctgaaagcc
     4201 ttatcatcta cgcaaaatgg aactcctagg tcaagacccg ccgcgttggg gcattccggc
     4261 atggagcaga tggacgagct gcgactggcg gcgcgcgccg gtcttcgcga agatcgagcg
     4321 caggtgcgag cgcgccgtat tatgcgcgat gccgagccgg tcggccgcct cgaccagcga
     4381 gccgccgcct gccagcgcca cttggtccc
//
LOCUS       DATMSU010000405         1660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_54357, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000405 DATMSU010000000
VERSION     DATMSU010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54357"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VN034_10905"
     CDS             <1..1229
                     /locus_tag="VN034_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HWV61155.1"
                     /translation="VTTLAPDGPGSLKAAIETPGPRIIVFEVGGVIDMGRKTIEIKHP
                     YLTIAGQTAPGPGITLIRTGIDVKTHDVVMRHLRVYTGADGQPKRSGWEADTFSTVAA
                     RNVIVDHCTFLWGIDENMSASGPRFTGKSVAEWREGTSHNITFSNNLAAEGLADASHP
                     KGEHSKGSLIHDNATGIVFYRNVWAHNVERNPLIKGGGQALMINNLIYNPQHRAVHYN
                     LMNLEWVGHDYVTGQITAIGNVLRGGNDTDEGLPFLMLGGDGDLDYYGRDNLAVDRHG
                     NALPEFGRYGETQAKLVRAKAPLAPTDGYRILPVREVETSVLATAGARPWARDAEEIR
                     ILFFVAEGRGDVIDDEKEVSAYPKVAAPVRARFVEKDWDLATMEPKSGRYPGQTAPLP
                     QEHLSARDRESRGERP"
     gene            1226..1627
                     /locus_tag="VN034_10910"
     CDS             1226..1627
                     /locus_tag="VN034_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV61156.1"
                     /translation="MSVLLAILLANAAAPAMVVIDEAATVREEAPPHGKIGMSTAYRI
                     SDAVPAPRSFEFRKRALHVGAAIGIHPIDHDEIYYVLSGTGIVHSDGEEKALKAGTAA
                     WLYKGAQVGIRQTGGEPLVLIIAYPNQAAPR"
BASE COUNT          307 a          552 c          561 g          240 t
ORIGIN      
        1 gcgtgaccac gctcgcgccc gatggtcccg ggtcgctgaa ggcggcgatc gagacgccgg
       61 ggccgcgcat catcgtgttc gaggtcggcg gcgtgatcga catgggccgc aagacgatcg
      121 agatcaaaca tccctatctg acgatcgccg gccagaccgc gccgggaccg gggatcaccc
      181 tgatccgcac cggcatcgac gtgaagacgc acgacgtcgt gatgcgccac ctgcgcgtct
      241 ataccggcgc cgacggccag ccgaagcgca gcgggtggga ggccgacacc ttctcgaccg
      301 tcgcggcgcg gaacgtcatc gtcgaccatt gcaccttcct gtgggggatc gacgagaata
      361 tgtcggcgtc gggcccgcgc ttcaccggca agagcgtcgc ggagtggcgc gagggaacga
      421 gccacaacat caccttttcg aacaatctcg ccgccgaagg gctcgccgac gcgagccatc
      481 ccaagggcga gcacagcaaa ggatcgctga tccacgacaa tgcgacgggc atcgtcttct
      541 accgcaatgt ctgggcgcat aatgtcgagc gcaatccgct gatcaagggc ggcgggcagg
      601 cgctgatgat caacaatctg atctacaatc cgcagcaccg cgcggtgcat tataatctga
      661 tgaacctcga gtgggtcggc catgattatg tcaccgggca gatcaccgcg atcggcaatg
      721 tcctgcgcgg cggcaacgac accgacgagg gcctcccctt cctgatgctc ggcggcgacg
      781 gcgacctcga ctattatggc cgggacaatc tggcggtcga ccgccacggc aacgcgcttc
      841 ccgaattcgg ccgctacggc gaaacgcagg cgaagctcgt ccgggcgaaa gcgccgctcg
      901 cgccgacgga cgggtatcgc atcctgccgg tgcgcgaggt cgaaacctcg gtgctggcga
      961 ccgcgggcgc gcggccgtgg gcgcgcgatg ccgaggagat ccgcatcctc tttttcgttg
     1021 ccgaagggcg cggcgacgtc atcgacgacg aaaaggaggt cagcgcctat cccaaggtcg
     1081 cagcgccggt acgcgcgcgc ttcgtcgaaa aggattggga cctcgcgacg atggaaccga
     1141 agtctggccg ctaccccggc cagaccgcgc cgctgccgca ggaacatctg tcggcgcgcg
     1201 atcgcgaatc gcggggcgaa aggccatgag cgtgctgctc gcgatcctgc tcgcgaacgc
     1261 cgccgccccc gcgatggtgg tgatcgacga agccgcgacg gtgcgcgagg aggcgccgcc
     1321 gcatgggaaa atcggcatgt cgaccgcgta ccggatcagc gatgcggtgc ccgcgccgcg
     1381 cagcttcgag ttccgcaagc gcgcgctgca cgtcggcgcg gcgatcggca tccatccaat
     1441 cgaccatgac gagatttatt atgtcctgtc gggcaccggc atcgtccatt cggacggcga
     1501 ggaaaaggcg ctgaaagccg ggaccgcggc gtggctctac aagggcgcgc aggtcggcat
     1561 ccgccagacg ggcggcgagc cgctggtgct gatcatcgcc tatccgaacc aggcggcccc
     1621 gcgatgaccg tcggccgccg cgcgctgctg accgcgagcc
//
LOCUS       DATMSU010000406         9928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_73344, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000406 DATMSU010000000
VERSION     DATMSU010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73344"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VN034_10915"
     CDS             complement(<1..308)
                     /locus_tag="VN034_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61157.1"
                     /translation="MNAAATEIYVSGPSQQQALFSHLIKSAARRASSMLAPASEAARL
                     RLPADDGMTAIERLEHSPLAEDQIMATALRLSPSAARPKAIASALQRHFTTPPGWLAV
                     "
     gene            complement(565..1308)
                     /locus_tag="VN034_10920"
     CDS             complement(565..1308)
                     /locus_tag="VN034_10920"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWV61158.1"
                     /translation="MHRCAPALRIVAAPAPPLTAVPDALVFTSLNGVRLHRFSPALAG
                     LPVFAVGDHSARYARAQGYRRVTSAAGNIVDLCQAIQLGLRAPADILHIGAVSPAGDL
                     AAMLGSSYRVRRLPVYSVEESDPRDLEWIAGRLAEIGHILVHSPRAGDLVARWLGATL
                     PHWSGVAHCISPAAASPLRSLPGVSTRISDRPNERSLLAALVENRQPEAPLARQRTVP
                     IEPTASSGHEQAAPVMLDFPGVSAILRSP"
     gene            complement(1384..1716)
                     /locus_tag="VN034_10925"
     CDS             complement(1384..1716)
                     /locus_tag="VN034_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61159.1"
                     /translation="MSEAEAFGLLQNRRAAFTAAAAPVLVGVDIDSVSGAAAADRLLD
                     MTIAAYEGQLSPETAARGFPRGAYSLFGDNLVSLLKDVLGTSLPFAFLARCVDSYWRS
                     AVRAMAPE"
     gene            complement(2013..2459)
                     /locus_tag="VN034_10930"
     CDS             complement(2013..2459)
                     /locus_tag="VN034_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV61160.1"
                     /translation="MPALDDTDRRILNSLRLNARITNSDLAEEVGLSPSACLRRVRLL
                     EQSGVIRGYTTIVAGGSPEEGMIAIVQVELERQSEEYLLRFEAALRRHAEIQEWFLMT
                     GDGDYILRVQIGGIDDYGAFHRDVLSRLPGVARITSSFAMRSHRRR"
     gene            2581..3720
                     /gene="alr"
                     /locus_tag="VN034_10935"
     CDS             2581..3720
                     /gene="alr"
                     /locus_tag="VN034_10935"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWV61161.1"
                     /translation="MEPPAPPHSSRLIVDCDAIAANFQFLATRVAPAECGAVIKADAY
                     GLGIADVAPALLHAGCRSFFVARLCEAERLRRLVGSGCNITILNGLDPGSEAACAVAG
                     FVPVLNSVSQFAAWRAHARSAARPLPAALQIDTGMSRLGLSPDAALAAANDPELAEEL
                     DLKLLMTHLACGDEPVRSVSMEQLNLFEAVAAHFPAVPRSIANSAAAFLPRAFHCDVV
                     RTGVALFGVSPLPEAMNLQPAVRLDARILQIRAIEAGTGVGYGLDHVATTSQRLATIA
                     IGYADGWPRSLGGKGAAWHQGVRLPIVGRISMDSLTIDIGGLADGSVSEGDFVELLGP
                     SQTLADVARDAGTIAYEILTGLGARHARFAIENGQTQAAHTGATA"
     gene            3717..4973
                     /locus_tag="VN034_10940"
     CDS             3717..4973
                     /locus_tag="VN034_10940"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HWV61162.1"
                     /translation="MRVVVLGAGVVGVASAYYLADAGHEVVLVDRQPGPALETSFANA
                     GEISPGYASPWAAPGIPVKAMRWLFMKHAPLILSPRIDMAMLRWILAMLRNCTPARYA
                     LNKSRMVRLAEYSRDELLALRGRLALDYDQRSQGTLQLFRTQKQFDDSADDIAVLEQY
                     GVAYELLGREGCVGAEPGLSAARGRFVGGLRLPGDETGDCHLFTRQLAGRLTAMGVEL
                     RYNLPIAGLAMDAGRVAGVRTEAGLIRGDIYLVALGSHSPALLRQAGLSLPVYPVKGY
                     SITLPIADDACAPASTLLDESYKIAITRLGDRIRVGGMAELSGFDAALPPQREATLRH
                     SLHDLFPGGADMTAPSHFWSGFRPMTPDSTPVIGATPVSNLFLNTGHGTLGWTMACGS
                     GRLVSSLIGGERPPLDPAGLGIERYM"
     gene            4994..5341
                     /locus_tag="VN034_10945"
     CDS             4994..5341
                     /locus_tag="VN034_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWV61163.1"
                     /translation="MSIERLHSDSRMSQAVIHQGLVHLAGQIGAPGEDTAAQTRAVLG
                     KIDALLAEAGTDKSHILTATIWLADMEDFGAMNAVWDEWVGGANAPARATGGVKLATP
                     EYRVEIIVIAALP"
     gene            5646..6056
                     /gene="rnk"
                     /locus_tag="VN034_10950"
     CDS             5646..6056
                     /gene="rnk"
                     /locus_tag="VN034_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HWV61164.1"
                     /translation="MTRQKCSKRPPIHMIGSEADVLADLALAAAERSPKVSEMLLDET
                     SRAKFYTRERIPRDVVVMGATVEFDDANGSTRSVKLVYPNDADISAGRISILTPVGAG
                     LIGLREGQSILWPDREGHERELTIVRVKQEVGAS"
     gene            6137..7003
                     /locus_tag="VN034_10955"
     CDS             6137..7003
                     /locus_tag="VN034_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWV61165.1"
                     /translation="MTAKSPPTRILVATDLSCRCDRALDRAVALAQRWAAELVAVAVM
                     ETSATDMLEARPRQWRRQADPAERMRWRLARDVGSAAANIRTVVDEGDPASKINAVAE
                     REHCDLIVTGFGHDETLGRIFLGSTVDRLVRETRIPVLVVRERTVGAYARVLATTDAS
                     EESLEAIRVASAFFPGAAFTFFHAFDYPYSSLLDNRDFTRETAEKEAELTQLLENGGF
                     GRDPLKQDVRPVARFGSPEASLPDYVEDHRIDLIVVGSNGHGALFDALIGSTHRKLLD
                     TLEGDMLIVRES"
     gene            complement(7059..7550)
                     /locus_tag="VN034_10960"
     CDS             complement(7059..7550)
                     /locus_tag="VN034_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV61166.1"
                     /translation="MANDHSLDRIDRKILRFLQEEARLTNQALAERIALSPSACLARV
                     RRLEELGIIQGYHARLDPFRVDVGLVLFVEITLEGRAPDEQARFQKALSAIPEIVEAS
                     HVSGRIDYLLKVVVRNMQHWTDLREALVGGDYGIGSVTTHVLMDKPKVFTGYPVAEQA
                     LDR"
     gene            complement(7981..8928)
                     /locus_tag="VN034_10965"
     CDS             complement(7981..8928)
                     /locus_tag="VN034_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWV61167.1"
                     /translation="MIHRDGGVIFDEPSLCCLAGLRDQPRFVAAGAPVFAMTDRTPAG
                     MRVRRPLRRGVLQDIGAATHLLRYAASQVIKRRSFRSPRVMIGLPADVTQAERNAMLT
                     AARDAALNPVSFVPEPLAAAIGANLKIEAPQGTMIVECGAGTTEVAVLSLGGVCLTRS
                     LRGGGAELDSAIIDYLHSRHKLLVGRSSAERIKYALGEACTRGGAADTIAVKGRSLMS
                     GLPAVIRIAPIEFADVIERHAQHIVDLILELLAETPPELSKDIHGQGILLTGGGAATP
                     ALREKIEAGTGLHVAIAEDSGLCVARGLFRLLQGGAAAN"
     gene            complement(9390..>9928)
                     /locus_tag="VN034_10970"
     CDS             complement(9390..>9928)
                     /locus_tag="VN034_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV61168.1"
                     /translation="IESEVGKGTTVRILFPVSTSDDAELPVAGPKQSVERGEAHILLV
                     EDDAEVRALARENLENAGYRVTVAENGDAGLVALEHLLATDMVDLVFTDLVMPGNLDG
                     IALAEAVRRLAPQVPVLMTTGYNEDLVVEGPLRSERDVLGKPYRRADLLDRIAQALAH
                     RDENSARRRPSDFGAAEE"
BASE COUNT         1802 a         3182 c         3148 g         1796 t
ORIGIN      
        1 tcgacggcga gccatcccgg cggggtcgtg aaatggcgtt gcagcgcgct cgcgatcgcc
       61 ttgggacgcg ccgcactcgg cgacagtcgg agcgcggtgg ccataatctg atcttcggcg
      121 agcggggaat gctccagccg ctctatcgct gtcatgccgt cgtcggcagg caagcgcagc
      181 cgtgcagcct cagaggcggg cgcaagcatc gaactcgcac ggcgcgcggc acttttgatc
      241 aaatggctga acagagcttg ctgttgcgaa ggaccactca cgtaaatctc cgtcgccgcc
      301 gcgttcaacg cgcggcggcg tcttgtggca gtagctgaac caatgcctgc ggaagcgcag
      361 gctattctgg tggatgtcag ttggtcctaa ttgaccatgt cgccgcgccg cgaacagccg
      421 ccttggcgtg gctgatccgc gtcgatgcgc tgcctgtgat cagggaaaca ccccgggtcc
      481 gccacgcgcg gatccggggc ggctagcgtt cgagcagccg gcccacatag tgcgcaaagc
      541 gcgtaggaga tgaagcgttt tcgctcatgg tgaccttaag atggcgctga cgccggggaa
      601 gtcaagcatg accggcgcgg cctgttcgtg cccgcttgag gccgtgggct cgatcggaac
      661 cgtccgctgc cgagccaaag gggcctccgg ctggcgattc tcgaccaagg ccgcaagcag
      721 cgaccgctcg ttgggccgat ccgatatccg cgtcgagacc ccgggaagcg accggagggg
      781 agaggccgcc gctggagaga tgcaatgtgc cacacccgac cagtgcggca aggttgcacc
      841 gagccagcgc gcgacgagat cgcccgcgcg cggcgaatgc accaggatat ggccgatctc
      901 ggcaaggcgg cccgcgatcc attcgagatc gcgcgggtcg ctctcctcga cgctgtagac
      961 cggcaaacgc ctgacgcgat aagatgatcc gagcatggcg gccagatcgc ccgccggact
     1021 caccgctcca atatgaagga tgtcggccgg cgctctcagg ccgagctgta ttgcttggca
     1081 aagatcgacg atgtttccgg cggccgaggt cacccggcgg tagccctgcg cccgcgcgta
     1141 tcgcgcgcta tggtcaccga cagcgaatac gggaagaccg gcgagcgccg gagaaaagcg
     1201 gtgaagcctg acaccgttga ggctcgtgaa gacgagagcg tccggaaccg ccgtgagagg
     1261 cggcgccggc gccgcgacga tgcgcagcgc gggagcacag cggtgtatag ccggcgccgg
     1321 cgaggcgctt ggccgtcaac cggttgaacg ggtcggtgcg ggtcacccag acgaggcccc
     1381 gggtcattcg ggggccatcg cgcgtaccgc gctacgccag taggaatcga cgcatcgcgc
     1441 caggaacgcg aaaggcaatg aagtgccgag gacgtccttc aacaacgata cgagattgtc
     1501 accgaaaagg ctgtaggctc cgcgcgggaa cccgcgcgcg gccgtctcgg gagacaattg
     1561 cccttcgtag gccgcgattg tcatgtcgag caatctatcg gcggcggcag cgccgctcac
     1621 gctatcgata tcaactccga cgagaacagg cgccgcagct gccgtgaatg ccgcgcgtct
     1681 gttttgcaaa aggccgaaag cctcggcctc ggacatgggg gtatccatta tgcactccgg
     1741 gcgtgtaaac gccgccttga acccgcatat tgcgggcttc ctgcttgggg aaatgatcgg
     1801 ggaggatctc ggggccggat cccgctgtcg cgcctcggga acggccccgt ctcgcgtcaa
     1861 gcggcggggc gacgttccga ggcttggccg cgcgtcagca ggctgccgcc atgggcaaga
     1921 aaacggagaa ccgttggaaa catcatgccg cagtatagca tgaccgcagg aaagggccaa
     1981 atcggcaacg cccaccttcc caccgggcgc ctttacctcc tgcggtgact gcgcatggcg
     2041 aaactcgacg tgatgcgggc gacgccgggc aatcgcgaca agacgtcccg gtggaatgcg
     2101 ccataatcgt cgataccgcc aatctggacg cgaaggatat agtcgccatc gccggtcatc
     2161 aggaaccact cctggatttc cgcgtgccgg cggagcgcgg cctcgaaacg taagagatat
     2221 tcctccgact ggcgctcgag ttccacctgc acgattgcga tcatcccctc ctcgggcgag
     2281 ccgccagcca cgatcgtcgt ataacccctg atcacgcccg actgctcgag gagccgaacc
     2341 cgccgcaagc atgcggaggg ggaaagtccg acctcttccg ccaagtcgct attagtgatc
     2401 cgggcgttga gccgcagact gttcaggatg cgacgatccg tatcgtccag ggccggcatg
     2461 tggattcctc catttattcg cagatcatgt cagcatactg cgaatttagc attaatgttg
     2521 gtggattcag cgaaaaattg cgcaggaaac gcgttatcct cggctgcgag ggagatcaaa
     2581 ttggaaccgc ctgcgccgcc ccactcgagc cgccttatcg tcgattgcga tgcgatcgct
     2641 gcgaatttcc aatttctcgc gacgcgggtc gcgcctgcag agtgcggggc ggtgatcaag
     2701 gccgacgctt atggtttagg gatcgcggac gtcgctcccg ccctccttca tgctggttgc
     2761 cgcagcttct tcgttgcgcg tttgtgcgaa gccgagcggc ttcggcgcct cgttggatcg
     2821 ggctgcaaca tcacaatcct caatggcctt gaccccggca gcgaagcggc ctgcgccgtt
     2881 gcaggcttcg ttccggttct gaattcggtc tcgcagtttg ccgcctggcg agcgcatgcc
     2941 cgctcagccg cgcggccctt gccggccgcc ctgcagatcg acaccggtat gtcccgcctc
     3001 ggcttgtctc ccgatgcagc actggccgcc gcaaacgacc ccgaattggc ggaggaactc
     3061 gacctcaagc tcctgatgac gcacctcgcc tgcggcgatg agcccgttcg cagcgtaagc
     3121 atggaacaac tcaacctgtt cgaggccgta gccgcgcatt ttcccgcggt gccgaggtcc
     3181 atcgcgaaca gtgccgccgc cttcctgccg cgcgccttcc actgcgacgt ggtgcgtaca
     3241 ggtgtcgcgc tcttcggagt ctctcccctg ccggaggcca tgaacttgca gcccgcggtg
     3301 cgtctcgatg cccgaatact gcaaatccgc gcgattgagg cgggcacggg ggtcggctat
     3361 ggcctcgatc atgtcgcgac cacctcacag cgcctcgcaa ccattgcgat tggctatgcc
     3421 gacggttggc cgcggagtct cggaggcaag ggcgcggcct ggcaccaagg cgtgcggctt
     3481 cccatcgtcg ggcgcatttc catggacagc ctcacgatcg acatcggggg gcttgccgac
     3541 ggcagcgttt cggaaggcga ctttgtggag ttgctgggtc cttcgcaaac gcttgcagat
     3601 gttgcgcgcg atgcgggcac catcgcttat gaaatcctga ccggcctcgg ggcccggcac
     3661 gctcgtttcg ctatcgaaaa cgggcagacg caggccgcgc acaccggggc aacggcgtga
     3721 gagtcgttgt tcttggcgcc ggggtggtcg gcgttgcgtc cgcttattac ctggccgatg
     3781 cgggccatga ggtcgtgctg gtcgatcggc agcctggacc cgcgctcgaa acgagcttcg
     3841 ccaacgccgg ggagatttcc cccggttatg cgtcgccttg ggcggccccc ggcataccgg
     3901 tcaaggcgat gcgctggctg tttatgaaac atgcgccgct gatccttagt ccccgcatcg
     3961 atatggcgat gctgcggtgg attctggcga tgctgcgaaa ctgcacgccc gcccgctatg
     4021 cgctcaacaa gagccgtatg gtgcgccttg ccgaatatag ccgcgacgaa ctgctcgcgc
     4081 tgcgcggccg gctcgcgctc gactatgatc agcgctctca aggtacgctc cagctgttcc
     4141 ggacccagaa gcagttcgac gacagcgccg acgatatcgc ggtgctcgaa caatatggcg
     4201 tcgcgtacga actgctcggc cgcgaaggct gcgtcggcgc cgagcccggc ctgtccgccg
     4261 cccggggccg cttcgtcggc ggccttcgtc tgccgggcga tgaaacgggc gattgtcacc
     4321 tgttcacgcg tcagctcgcc ggtcgtctaa ccgcgatggg cgtcgaactt cgctataatt
     4381 tgcctatcgc cggactcgca atggacgccg gccgcgtcgc gggtgtccgg accgaggccg
     4441 gactgatccg cggagacatc tatttagtgg cactcggcag tcactcgccg gcactgctcc
     4501 gtcaggccgg gctgtcgcta ccggtctatc ctgtaaaggg ctattcgatc accctgccca
     4561 tagccgacga cgcctgtgcg ccggcgtcga ccctgctcga cgaaagttac aagatcgcga
     4621 ttacccggct gggcgaccgc atccgggtgg ggggcatggc ggaactgtcc ggcttcgatg
     4681 ccgcgctgcc gccccagcgg gaagcgacgt tgcgccattc gctgcacgac ctgtttcccg
     4741 gcggcgccga tatgacggcg ccatcgcatt tctggagcgg atttcggccg atgacgcccg
     4801 acagcacccc ggtcatcggc gcaaccccgg tttcgaacct gtttctcaac accgggcacg
     4861 gtacattggg ctggacgatg gcgtgcggat cgggacggct tgtatcgtcg ctgatcggcg
     4921 gcgagcgccc cccgctcgac cccgccgggc tcggcataga gcgctacatg tgaactcata
     4981 agaaaggaat attatgtcca tcgaacgact ccactcagac tcgcgcatga gccaggccgt
     5041 gatccatcag ggccttgtcc atctcgccgg ccagatcggc gctccgggag aagatacggc
     5101 cgcacaaacc cgcgcggtgc tcggcaagat cgacgctctg ctcgccgaag cggggaccga
     5161 caagtcgcat atcctgacgg cgacgatctg gctcgccgac atggaggact tcggcgcgat
     5221 gaatgccgtc tgggacgaat gggtcggtgg cgccaacgcc cccgcacgcg ccaccggcgg
     5281 ggtcaagctt gcgacgcctg agtatcgcgt cgaaatcatc gtgatcgccg cccttccctg
     5341 atcgagggcc ataccatagc gggccgcgcc cggaggtggc ccggcaccct aaaacccatg
     5401 ccgccgcagc ccctgcacag ggcgggcacg gctgcagcgg ttgactcgac tcccgaagcg
     5461 gcgcatcatg tctccatgca caatgcacag catctttgcc gtttccgcag cgaaagtgga
     5521 cagcttctcg cgggctagtt gcccccggtc tcaaggtgcc cgcgcccctg acccgggggc
     5581 caccctctcc taaaaattga acgagcaccg ccgcgtgcgt gcgctcgcct gcgaaagttg
     5641 gaaaaatgac aagacagaag tgctccaaac gccctcctat tcatatgatt ggcagcgaag
     5701 ccgatgtgct ggccgatctg gccctcgccg ccgccgagcg gtcgcccaag gtctccgaaa
     5761 tgcttctcga cgagacgtcg cgtgcgaaat tctacacccg tgagcgcatt ccccgggacg
     5821 tcgtcgtcat gggtgcgaca gtggaattcg atgacgccaa tggctcgacc cgttcggtga
     5881 agcttgtcta tccgaatgac gccgatatct ccgccgggcg aatttcgatc ctgacgccgg
     5941 tcggcgcagg actgatcggc cttcgcgaag ggcagtccat cttgtggccg gaccgcgaag
     6001 gccatgagcg cgagttgacg atcgtgcgcg taaagcaaga agtcggtgcg tcctgaacca
     6061 cggctcgcgg gcggagcgcg agtgacgtac catcgctctc cagaccggcc gcgagtattt
     6121 tgattggagc tctcagatga ccgccaaatc cccgcctacc cggatccttg tcgcaacgga
     6181 tctcagctgc cgatgcgacc gcgcgctcga ccgcgccgta gcactcgcgc agcggtgggc
     6241 agctgaactg gtcgcggtcg cggttatgga aacgagtgcg accgacatgc tcgaagcgcg
     6301 cccgcgccaa tggcgccggc aggccgatcc ggccgagcgc atgcgatggc gtcttgcccg
     6361 cgatgtcggg agcgccgcag ccaatatccg gacggtcgtc gacgagggag atccggcttc
     6421 caagatcaac gcggtcgcgg agcgcgagca ttgcgacctg atcgtgacgg gctttggcca
     6481 tgacgagacg ctcggccgca tcttccttgg cagcaccgtc gatcggctgg ttcgcgaaac
     6541 ccgcattccc gttctcgtcg tgcgcgaacg aaccgtcgga gcctacgcac gggtcctcgc
     6601 cacgaccgat gcttcggagg aatcgctgga ggcgatacgt gttgcatccg cgtttttccc
     6661 cggggccgcg tttaccttct ttcacgcttt cgattatcct tattccagcc tgctggataa
     6721 tcgcgacttc acgcgcgaga cggcggaaaa agaagcggaa ttgacccagc ttctggaaaa
     6781 tggcggtttc gggcgcgacc cgctcaagca ggacgtcagg ccggtcgcca gatttggatc
     6841 gcccgaagcc tcgctccccg attatgtgga agatcaccgc atcgatctca tagtggtagg
     6901 cagcaacggg catggggcgc tgttcgatgc cttgattggg agcacccacc gcaaacttct
     6961 cgatacactc gagggtgata tgttgatcgt gcgcgagagc tgatcaggta gcggtctgtc
     7021 caccgcaccg gcgcgcgatt ggttgccgca agccgccgct aacggtcgag ggcctgctcg
     7081 gcgaccggat aacccgtgaa gaccttgggc ttgtccatga gaacatgggt cgtcacgctg
     7141 ccgatcccat agtcgccgcc gaccagcgct tcgcgaaggt cggtccaatg ctgcatattg
     7201 cgaacaacca ccttcaggag atagtcgata cggccgctca catgcgaggc ctcgacgatt
     7261 tcgggaatcg cagaaagcgc tttttgaaaa cgcgcctgct cgtcgggcgc ccgtccctcg
     7321 agagtgatct cgacgaacag gacaagaccg acgtcgacgc gaaaagggtc aaggcgcgcg
     7381 tgatagccct gaataatacc cagctcctca agccgccgca cgcgcgcgag gcaggcgctc
     7441 ggcgacagtg cgatgcgttc cgcgagcgcc tggttggtga gccgcgcttc ctcttggaga
     7501 aaccggagaa ttttccgatc gatgcggtcg agtgaatgat cgttggccat cgtttcgcac
     7561 gaccgcagaa actgcgggcc gcgtcaaggt cttctgcgcc acctgcggcg cgaattcgtt
     7621 tacgatcctg cgtatcggca ttgcgccgac ccgcgtcgtc ttcgccatgg cacgcaacgg
     7681 gctcttgccg cggcgccttg cggaggtttc gcgcgcgggt gtcccggtcc gcaccacgct
     7741 ggtcaccacg cttctggtat cgacgatcgc ggcgttcctg ccgctcggcg agatcgtcgc
     7801 gcttgccaac gcgggcacgc ttctcgcttt ctgttcgcca gtctccccag cgccacgcac
     7861 atctggttcc ttgtctggaa cgacgaggtc attctgggct atttcctcat tcgccggcgc
     7921 ggccgccgac aggagaaagg ccggccagtc ctccagcgtc ccttctattg cgcgtcacga
     7981 tcagttcgcc gcggccccgc cttggagaag gcgaaagagc ccccgggcga cgcacaggcc
     8041 ggaatcttcc gcgatcgcca catgaagacc ggtgccagcc tcgatcttct cccgaagcgc
     8101 gggcgttgct gcgccgccgc cggtaagcaa tatgccttgg ccgtgaatat ccttgctcag
     8161 ctcgggcggc gtttcggcaa gcagttcgag gatcaggtcg acgatgtgct gcgcatgtct
     8221 ttcgatcacg tcggcgaact cgatcggcgc aatccggatc acggccggca atcccgacat
     8281 gaggctccgt cccttgactg cgatggtgtc ggcagccccg cctcgcgtac aggcttcgcc
     8341 aagcgcatat ttgatgcgct cggcgctgct gcggccaacg agcagcttat gtcgggaatg
     8401 aagataatcg atgatcgccg agtcgagttc ggcgccgccc ccgcgcagcg agcgtgtgag
     8461 gcagacccct ccgagcgaca agacggcaac ctcggtcgtc ccggcaccgc actcgacgat
     8521 catcgttcct tgcggagctt cgatcttgag attcgcgccg atcgccgcgg cgagcggctc
     8581 cggtacgaag gacactgggt tgagtgccgc gtcccgcgcc gcggtgagca tggcgtttcg
     8641 ttcggcctgc gtgacgtccg ccggcaatcc gatcatgacg cgcggggagc gaaacgaacg
     8701 gcgtttgatc acttgcgaag cggcatagcg aagcaaatgc gtggcggctc cgatatcctg
     8761 gagaacccca cgcctcagcg gccgccgaac ccgcattcca gccggcgttc ggtcggtcat
     8821 tgcaaaaacg ggcgcaccgg cggccacgaa gcggggctga tcgcgcagtc ccgcgaggca
     8881 gcagagcgag ggctcgtcga atataacgcc gccgtcgcgg tggatcatgc gtaaattcgc
     8941 cgtcccgaaa tcaatcgcga tttcggccgg tcggccggtg agattcgagg tcatgcggcg
     9001 cctctagcaa agccgtccat atctgtcgcg gtgtaaccag acgaatcgtc caggagggct
     9061 ttatcggctc catgaaagtt atcgaattta agacggatgg ccagccttag cgacaggcgt
     9121 caaacccgaa actggccgac cggagacagt ctgctgatgg catcagaatg caaggagctg
     9181 ccattaggtc cgttgcggca gattgcagct ccggcttcgt tggcgagaat ggtcagcgac
     9241 ggtcccgcgg tctggacaat ttacaggtgc gacactgcaa gtgcgcttgg gtgcgaggtc
     9301 gcaacgtcag ccgttgaggc attgaccgag accgggggct tggtttctga agtgcaacag
     9361 gccgtttacc caccgggcga ttgaaacgat cattcctcgg cagcaccgaa gtcagacggg
     9421 cgtcgccgag cagaattttc atccctgtgc gccagcgcct gtgcgatgcg atcgagcagg
     9481 tcggcccggc gatagggttt gcccagcacg tcacgctcgc tgcgaagtgg tccttcgacg
     9541 actaagtcct cgttatagcc cgtggtcatc aacaccggca cttgaggcgc gagccgccgg
     9601 actgcttccg cgagcgcaat gccatccagg tttccgggca tcaccaggtc ggtgaacaca
     9661 aggtccacca tgtcggtggc aagcagatgc tcaagcgcca cgagacccgc gtcaccattc
     9721 tcggcgaccg ttacgcgata accagcattc tcgagatttt cgcgcgccaa cgcgcggact
     9781 tcggcatcgt cttcgaccaa gagaatatgg gcctcgccgc gttcgacaga ctgtttcgga
     9841 cccgcaaccg gcaattcggc atcgtcagat gtcgagacgg ggaaaagaat gcgtacggtg
     9901 gtgcccttgc cgacctcgct ctcgattt
//
LOCUS       DATMSU010000407         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_25681, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000407 DATMSU010000000
VERSION     DATMSU010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25681"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VN034_10975"
     CDS             <1..190
                     /locus_tag="VN034_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HWV61169.1"
                     /translation="VAHPAGGTPADMRLTFWPRRRAVRAAMAEILAWDCERVVMAHGL
                     PYEQGGAAELRRAFGWAL"
     gene            complement(291..1379)
                     /locus_tag="VN034_10980"
     CDS             complement(291..1379)
                     /locus_tag="VN034_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWV61170.1"
                     /translation="MNTLVDRLSVPAAAATPKSAIADDMAMIRAASELTRDLVQPSAR
                     IYWTDFLASTFIGYAGIAAAILAPSTAWMLVAAVIAVVALYRAGSFIHELTHIRKNAL
                     PGFRLAWNILVGVPLLIPSFMYEGIHSLHHNRTKYGTVEDPEYLPLALMKPWTVPLFV
                     VAAAFAPLALVFRYGVLTPLSFLIPPLRRVVVERYSGMIINPLFRRKPPEGEFRRQWA
                     WQEGGAWAWSTLLIAAGVFGWVPLRALAIFGAIASATLVLNQIRTLVAHLWENDGEVL
                     TVTGQFLDSVNVPPPGLLPELWAPVGLRYHALHHLLPGVPYHSLAEAHRRLKHALPSD
                     SQYHGANYDSLPKLVMNLVAGSARGSAA"
     gene            1507..2673
                     /locus_tag="VN034_10985"
     CDS             1507..2673
                     /locus_tag="VN034_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWV61171.1"
                     /translation="MSGHSHGPITIHPVKDKNDLREFVELAYRLNRGDPAWVPPLKGE
                     VYGLLTPGKNPWFEHGKAQYFLARRDGRTIGRISAHIDELALAQPAEQGMGPGTGNWG
                     LLEAEDEESAQALIAAAEDWLRTQGMTRALGPLSISIWDEPGLLIEGFDNPPTVMMGH
                     NSPLYRGWIEGTGYRPVKQLLNYDVAIGDGFPPIVNRIVAAGDKNARIRIRKVDKRRF
                     DAEAKLIMGLLNDAWSDNWGFVPLTDSEIAYVGKKLKDIVFEDLIRVAEVDGEPVAFM
                     IVLPNINQLLIDMDGSLLPFNWARLLWWLRKPKSRILRVPLMGVAKKLQNSRMASTLA
                     FMMIEYIRRDAVSDYGTERGDIGWVLDDNQGMNAVAEAIEAKVNRVYQIYDKML"
     gene            2750..>3055
                     /locus_tag="VN034_10990"
     CDS             2750..>3055
                     /locus_tag="VN034_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HWV61172.1"
                     /translation="MAVQYLAANRFGLGRRFDDPAIEDPKDWLVRQIGDYDPAPAAIA
                     GLAGREAIATAYAEYRDERQAMRREAKNAAMRDDDGVADKAMRRMARAGFRRHYGDAA
                     "
BASE COUNT          580 a          954 c         1043 g          478 t
ORIGIN      
        1 ggtcgcgcat ccggcggggg gcacccctgc cgacatgcgc ctgactttct ggccgcgccg
       61 ccgcgcggtg cgcgccgcga tggcggaaat cctcgcctgg gactgcgagc gcgtcgtgat
      121 ggcgcacggc ctcccctatg aacagggcgg cgcggccgaa ttgcgtcgcg cgttcggatg
      181 ggcgctttag ctagcccgcg ttcttgcgtg cggcacggcg aaagccgggg gcctggggtg
      241 catgatggcg acccccaccc cgaagccgat acgccgccgg caaatcgata tcaggcagcc
      301 gaaccccgcg cagaccccgc aaccagattc atgaccagct tcggcaggct gtcgtagttc
      361 gcgccatgat attgcgagtc cgacggcagc gcgtgcttca ggcggcggtg cgcctcggcg
      421 agcgaatgat aggggacgcc gggcagcaaa tggtgcagcg cgtgatagcg caacccgacc
      481 ggtgcccaca attcgggaag caggcccggc ggcgggacat tgacgctgtc gaggaactga
      541 ccggtcacgg tcagcacttc gccgtcattt tcccagagat gcgcgacgag cgtgcggatc
      601 tggttgagca ccaaggtcgc cgacgcgatc gcgccaaaaa tggccagcgc gcgcagcggc
      661 acccagccga acacgcccgc ggcgatcagc agggtcgacc acgcccaggc gccgccttcc
      721 tgccacgccc actggcggcg gaactcgccc tcgggcggct tgcggcggaa cagcgggttg
      781 atgatcatcc ccgaatagcg ttcgacgacg acccggcgca gcggcgggat caggaaggac
      841 agcggggtga gcacgccgta gcggaagacg agcgcgagcg gcgcgaaggc cgcggcgacg
      901 acgaacagcg gcaccgtcca cggcttcatc agcgcgagcg gcagatattc gggatcctcg
      961 accgtgccat atttggtgcg gttgtggtgc aggctgtgaa tgccctcata catgaacgac
     1021 gggatcagca gcggcacgcc gacgaggata ttccacgcga ggcggaagcc cggcagcgca
     1081 tttttgcgga tatgcgtcag ctcgtggatg aagctgcccg cgcggtagag cgccaccacg
     1141 gcgatgaccg ccgcgaccag catccacgcc gtcgagggcg ccaggatcgc cgccgcgatg
     1201 cccgcatagc cgatgaaggt cgaggcgagg aagtcggtcc aatagatgcg cgcgctcggc
     1261 tgcacgagat cgcgcgtcag ttccgacgcc gcgcggatca tcgccatatc gtcggcgatc
     1321 gcggacttcg gggtcgccgc cgcagcgggc accgacaggc ggtcgacaag ggtgttcatc
     1381 gctaacatag gcggctcttc atcatatttt catggcagca aaatggccga aagcgggtcg
     1441 ccagcgcccc atctatgccc taatgcctcg ccacaaatat aggctcggct tttcaggaaa
     1501 ccacccatga gcggacattc gcacggcccg atcaccattc atccggtcaa ggacaagaat
     1561 gatttgcgcg agttcgtgga gctcgcctat cgcctcaacc gcggcgatcc ggcgtgggtg
     1621 ccgccgctga agggcgaggt ctatggtctg ctcaccccgg gcaagaatcc gtggttcgag
     1681 catggcaagg cgcaatattt cctcgcgcgg cgggacggcc gcacgatcgg ccgcatttcg
     1741 gcgcatatcg acgagctcgc actcgcgcag cccgccgaac agggcatggg cccgggcacg
     1801 ggcaattggg ggctgctcga ggccgaggac gaggaaagcg cgcaggcgct gatcgctgcg
     1861 gccgaggact ggctgcggac ccaaggcatg acgcgcgcgc tcggcccgct gtcgatttcg
     1921 atctgggacg agccggggct gctgatcgag gggttcgaca acccgccgac ggtgatgatg
     1981 gggcataaca gcccgctcta tcgcgggtgg atcgagggca ccggctatag gccggtgaag
     2041 cagcttctga actatgatgt cgcgataggc gacggctttc cgccgatcgt caaccgcatc
     2101 gtcgcggcgg gcgacaagaa tgcgcgcatc cgcattcgca aggtcgacaa gcgccgtttc
     2161 gatgccgaag cgaagctgat catggggctg ctcaacgacg cctggtcgga caattggggc
     2221 tttgttccgc tgaccgacag cgagatcgcc tatgtcggca agaagctgaa ggacatcgtg
     2281 ttcgaggatc tgatccgcgt cgccgaagtc gacggcgaac ccgtcgcttt catgatcgtg
     2341 ctgccgaaca tcaatcagct cttgatcgac atggacggat cgctgctccc cttcaactgg
     2401 gcgcggctgc tctggtggct gcgcaagccc aagagccgca tcctgcgcgt gccgttgatg
     2461 ggcgtcgcga aaaagcttca gaacagccgc atggcgagca cgctggcgtt catgatgatc
     2521 gaatatatcc gccgcgacgc ggtgagcgat tacgggaccg agcgcggcga catcggctgg
     2581 gtgctcgacg acaatcaggg gatgaacgcg gtcgccgagg cgatcgaggc caaggtaaac
     2641 cgggtgtatc agatctacga caaaatgctc tgatcgctgg gatagctttg cgacaaatgg
     2701 cggccatcgt gcgtaagtgt caggaccctt gggcacggaa aggtttgtca tggcagtcca
     2761 gtatcttgcc gccaatcgct tcgggctcgg ccgccgcttc gacgatccgg ccatcgagga
     2821 tccgaaagac tggctggtgc gccagatcgg cgattatgat cccgcccccg cagcgatcgc
     2881 cgggctggcg gggcgcgagg cgatcgcgac cgcctatgcc gaatatcgcg acgaacggca
     2941 ggcaatgcgc cgcgaagcga agaatgcggc gatgcgcgac gacgacgggg tggcggacaa
     3001 ggcgatgcgc cgaatggcgc gcgccggctt tcgccgccac tatggcgacg ccgcg
//
LOCUS       DATMSU010000408         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_167688,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000408 DATMSU010000000
VERSION     DATMSU010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167688"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VN034_10995"
     CDS             complement(<1..1150)
                     /locus_tag="VN034_10995"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWV61173.1"
                     /translation="MSTRTASDSNDPIVETRDQLAAPMIKGEKPRDRWRIGTEHEKFV
                     YRTADHRAPSYDEPGGIHDLLMALTRFGWEPVIEGGKVIALSGNDGTVSLEPAGQLEL
                     SGAPLENLHQTCAETGRHLKQVKEVGAELGLGFLGLGMWPDKTRAELPVMPKGRYKIM
                     LDHMPRVGSLGLDMMLRTCTIQTNLDYASEADMVQKFRVSLALQPLATALFASSPFTE
                     GRPNGFMSYRSHIWTDTDPARTGMLPFVFDEGFGYERYVDYMLDVPMYFVFRDGQYID
                     AAGQSFRDFLKGELPALPGEKPRLSDWNDHLSTAFPEVRLKSFLEMRGADGGPWNRIC
                     ALPALWVGLLYDQGALDAAWDLVKGWSIAEQQSLRDTVPRDGLCAPAPG"
     gene            complement(1212..>2187)
                     /locus_tag="VN034_11000"
     CDS             complement(1212..>2187)
                     /locus_tag="VN034_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proprotein convertase, P"
                     /protein_id="HWV61174.1"
                     /translation="PALVCPVGTTMHDWDGVSWPAGTTSGSAALTAIGTANFNIAVSG
                     GSFLSNAAYGGQSPTRQNVVTGALAPAQYSIFQLVDFTSQTGTVTSTIALPTAVPGAQ
                     FRLFDVDYSAGQFADRVTVTGTYNGAAVTPILTNGTANYVIGNSAYGDILSADGSADG
                     TVTVTFNSPVDSITIEYGSHGLAPANPGQQGMAIHDIIFCRAATNLVIAKTSSVVSDP
                     VNGTANPKAIPGARMRYCILVTNNGSGTATAINLADPLPAGTSFIAGSLRSGTSCAGA
                     MSVEDDNASGADESDPFGASVAGATVAAATATLLPAAAMAIAFEVTLD"
BASE COUNT          338 a          681 c          768 g          400 t
ORIGIN      
        1 cgccgggcgc cggggcgcac agtccgtcgc ggggcaccgt gtcgcgcaag ctttgctgct
       61 ccgcgatgct ccagcccttg acgagatccc acgcggcgtc gagcgcgccc tggtcataga
      121 gcagcccgac ccacagcgcc ggcagcgcgc agatgcggtt ccacgggccg ccgtcggcac
      181 cgcgcatttc gaggaagctt ttgaggcgca cctcgggaaa ggcggtcgac agatggtcgt
      241 tccagtcgga gaggcgcggt ttctcgcccg gcagcgcggg cagctcgccc ttcagaaagt
      301 cgcggaagct ctgccccgcg gcgtcgatat attggccgtc gcgaaagacg aaatacatcg
      361 gcacatcgag catataatcg acatagcgtt cgtagccgaa tccttcgtcg aacacgaagg
      421 gcagcatgcc ggtgcgcgca gggtcggtgt cggtccagat gtgactgcgg tacgacataa
      481 aaccgttggg ccgcccttcg gtgaagggcg agctggcgaa gagtgcggtc gcgagcggct
      541 gcaacgcgag gctgacgcgg aatttctgca ccatgtcggc ctcgcttgca tagtcgaggt
      601 tggtctggat cgtgcaggtg cgcagcatca tgtcgagccc gagactgccg acgcgcggca
      661 tatggtcgag catgatcttg tagcgcccct tgggcatgac cggcagctcg gcgcgcgtct
      721 tgtcgggcca catgccgagc ccgaggaaac cgagcccgag ttcggcgccg acttccttca
      781 cctgcttcag gtggcggccg gtctcggcgc aggtctggtg gagattttcg agtggtgcgc
      841 cggacaattc caattggccc gcgggctcga ggctgacagt gccgtcattt cccgagagag
      901 cgatgacctt tccgccctcg atcaccggtt cccagccgaa gcgcgtcagc gccatgagca
      961 ggtcgtggat gccgccgggt tcgtcatagg agggcgcgcg atgatcggcg gtgcgataga
     1021 cgaatttctc gtgctcggtg ccgatgcgcc agcggtcccg gggcttttca cccttgatca
     1081 tgggcgccgc gagctggtcg cgggtttcga cgatgggatc gttgctgtcg gaggcggtgc
     1141 gcgtgctcat gtcgcgcatt tagactatcc ggtatagaat gcaatcgggg ccgatcgtca
     1201 gttgccgatc gtcagtcgag cgtgacctcg aacgcgatcg ccatcgcggc ggcgggcaac
     1261 agcgtcgcgg tggccgcggc gacggtcgcg cccgccaccg atgcgccgaa cggatcgctt
     1321 tcgtcggcgc cgctcgcatt gtcgtcttcc acgctcatcg cgcccgcgca gctcgtgccg
     1381 ctacgcagcg atccggcgat gaagctcgtt ccggcgggca gcggatcggc caggttgatg
     1441 gcggtcgcgg tgccgctgcc attgttggtg acgaggatgc aatatcgcat ccgggcgccc
     1501 ggaatcgcct tgggattggc cgtgccgttg accgggtcgc tgacgacgct gctcgtcttc
     1561 gcgatcacca ggttcgtcgc cgcgcggcag aagatgatgt cgtggatcgc catgccctgc
     1621 tgccccggat tggccggcgc gaggccgtgg ctgccatatt cgatcgtgat gctgtcgacg
     1681 ggcgagttga aggtcacggt gacggtgccg tcggcgctgc cgtcggccga tagtatgtcg
     1741 ccataggcgc tgttcccgat cacataattg gcggtgccgt tggtgagtat gggcgtgacg
     1801 gcggcgccgt tataggtgcc ggtgacggtg acacggtcgg cgaactggcc cgcggaataa
     1861 tcgacgtcga acagccggaa ctgcgcgccg ggcaccgcgg tcggcagcgc gatcgtcgag
     1921 gtgacggtcc ctgtctggct ggtgaaatcg acgagctgaa agatcgaata ttgcgccggt
     1981 gccagcgccc cggtgacgac attctgccgc gtcggcgact ggccgccata ggccgcgttg
     2041 ctgaggaaac tgccgccgct gaccgcgatg ttgaagttcg ccgtcccgat cgcggtcagc
     2101 gccgcgctgc cgctcgtcgt cccggcgggc cagctgacgc cgtcccagtc gtgcatcgtc
     2161 gtgccgaccg ggcagacgag cgccggc
//
LOCUS       DATMSU010000409         1688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_186972,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000409 DATMSU010000000
VERSION     DATMSU010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186972"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="VN034_11005"
     CDS             <1..431
                     /locus_tag="VN034_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61175.1"
                     /translation="STPARPSPIADTDLRQFVAIAGRKVAGRPVEGPYANADKVLLIR
                     RDDKGYPVVAGSMGFPVRQSLPAPPAGTLAVVRLHQRSETVVPGPTADDLAFVKANRL
                     PLFVIGEWARPAPMWEVAWVDGAVRFRTIGEIGEIGPWRD"
     gene            550..1224
                     /locus_tag="VN034_11010"
     CDS             550..1224
                     /locus_tag="VN034_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWV61176.1"
                     /translation="MSKFEPVAKAPVRIQQNILARSERRLLNWLCARLPAWVTPDQLT
                     TLGFAGAVLVAAGYLLSWFDSEWLGLSLAGYIVNWFGDSLDGSLARWRGIERPNYGYF
                     VDHSVDAIATLLMISAIGMSPYMRLDVALMGVIGYFLLSIHTFISAKILGEFRLSYMA
                     GGPTELRLMLMAMTISMPIIGGADIKGTNFSVFDLFAIVVSSILVTLFIVQTSATARM
                     LRRRGN"
     gene            complement(1254..>1688)
                     /locus_tag="VN034_11015"
     CDS             complement(1254..>1688)
                     /locus_tag="VN034_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HWV61177.1"
                     /translation="VADSDEVGLAALAPPHAAELYGLTLHGTGMEDADHNTTRFVVLA
                     REPLPDVAAISGPLMTTFMFEVKNIPAALYKALGGFATNGVNMTKLESYQTGDSFAAT
                     KFYADIVGAPGDERIDRALEELDFQTKSLRLLGTYAQARTRP"
BASE COUNT          261 a          578 c          544 g          305 t
ORIGIN      
        1 gctccacgcc cgcgcgcccg tcgccgatcg ccgacaccga tctgcgccaa ttcgtcgcga
       61 tcgcgggacg caaggtcgcc gggcggccgg tcgaaggacc ctatgcgaac gccgacaagg
      121 tgctgctgat ccgccgcgac gacaagggct atcccgtcgt ggcggggagc atgggtttcc
      181 cggtgcgcca gtcgcttccg gcaccgcccg ccggcacgct cgccgtcgtc cgcttgcacc
      241 agcgttccga aacggtcgtt cccggcccga ccgccgacga ccttgccttc gtgaaggcga
      301 accggctgcc gctgttcgtg atcggcgaat gggcccgccc cgccccgatg tgggaggtgg
      361 cgtgggtcga tggcgcggtc cgtttccgca cgatcggcga gatcggcgaa atcggcccct
      421 ggcgcgattg accggcacga aggcacgccg caccgaccgc gtcgcccttt cgcccgcacg
      481 actttgcatc tatgacggat cgatgacatg cgaccggcac ttttgtgacg gccggaacaa
      541 agggtcccca tgagcaagtt cgaacccgtc gccaaggcgc cggtccgcat ccagcagaat
      601 atcctcgcgc gcagcgagcg ccgccttctc aactggctgt gcgcgcgcct tcccgcatgg
      661 gtcacccccg accagctcac cacgctgggg ttcgccggcg cggtgctcgt cgcggcgggc
      721 tatctgctca gctggttcga cagcgaatgg ctcggcctct ccctcgccgg ctatatcgtc
      781 aactggttcg gcgattcgct cgacggcagc ctcgcgcgct ggcgcggcat cgaacgcccc
      841 aattacggtt atttcgtcga tcacagcgtc gatgcgatcg ccacgctgct gatgatcagc
      901 gcgatcggca tgagccccta tatgcggctc gacgtcgcgc tgatgggggt aatcggctat
      961 ttcctgctgt cgatccacac cttcatttcg gcgaagatcc tcggcgaatt ccgcctgtcc
     1021 tacatggcgg gcgggccgac cgagctgcgg ctgatgctga tggcgatgac gatttcgatg
     1081 ccgatcatcg gcggggccga cataaagggc accaattttt ccgtcttcga cctgttcgcg
     1141 atcgtcgtct cgagcatcct cgtcacgctg ttcatcgtcc agacctcggc gaccgcacgg
     1201 atgctccgcc gccggggcaa ctgagatcga agaagcggcg gcgtccgcca gcctcaggga
     1261 cgcgtccgcg cctgcgcgta ggtgccgagc agacgcagcg acttggtctg gaaatcgagc
     1321 tcctcgagcg cgcggtcgat ccgctcgtcg cccggcgcac cgacgatgtc ggcatagaat
     1381 ttggtcgccg caaaactgtc gccggtctga tagctttcga gcttggtcat gttgacgccg
     1441 ttggtcgcaa agccgccgag cgccttgtag agcgcggcgg gaatattctt cacctcgaac
     1501 atgaaggtcg tcatcagcgg cccggagatc gccgccacat cgggaagcgg ttcgcgcgcg
     1561 agcacgacga agcgcgtcgt gttgtggtcg gcgtcctcca tccccgtgcc atgcagcgtg
     1621 aggccgtata gttcggccgc gtgcggcggc gcgagcgcgg cgaggccgac ctcgtcgctg
     1681 tcggcgac
//
LOCUS       DATMSU010000410         6218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_82464, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000410 DATMSU010000000
VERSION     DATMSU010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82464"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="VN034_11020"
     CDS             complement(<1..1158)
                     /locus_tag="VN034_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWV61178.1"
                     /translation="MRFASTLLASAALLAAAPVHAKPADPAVAKKILKDSVAIPTVEG
                     RGKVPELAAYYAGVLKAAGYADADIEITPMGETATFAATLAGTTRQKPIVLLGHMDVV
                     EADPKDWTRDPFVPVEENGYIFGRGSEDNKFDVSMMVATMAQLKKDGFKPKRSIILLL
                     SGDEETSMTTTRALAAKYKGAEFALNGDGGGGLIAEDGKPQYYGLQAGEKTYADFTLE
                     VTNPGGHSSRPTGSNAIVQLSTALAKVGAYRFTPQQNELTKVGMPIVADQVGGDIGAA
                     LKAFAANPSDAKAIAAIRADPEYIGQIGTTCVPTLVKGGHAENALPQRATANINCRIF
                     PGVPVETVRAELEKAIADPAIKVKTDPDASASDASPLRPDVVAAVTKAVHAQ"
     gene            complement(1281..1940)
                     /gene="nth"
                     /locus_tag="VN034_11025"
     CDS             complement(1281..1940)
                     /gene="nth"
                     /locus_tag="VN034_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWV61179.1"
                     /translation="MKKADIFEFYRRLAELNPSPETELEYGNIYQLLVAVVLSAQATD
                     VGVNKATRALFRDVKTPQQMVDLGEEGLKQHIKTIGLFNAKAKNVIGLSEILVRDFGG
                     EVPADRDTLVELPGVGRKTANVVMNCAFGAETFAVDTHIFRVGNRTGLAPGKTPLAVE
                     KKLDKETPQPFRVGAHHWLILHGRYICKARTPECWRCPVADLCRYKPKTPAPKGWKAA
                     A"
     gene            complement(1937..2674)
                     /gene="dapB"
                     /locus_tag="VN034_11030"
     CDS             complement(1937..2674)
                     /gene="dapB"
                     /locus_tag="VN034_11030"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWV61180.1"
                     /translation="MTSIGIIGSEGRMGVALAAAISEAGQRSCGVDRGGNVVRLASES
                     DVLVDFSSPAALEATLDACVAAKKPIVIGTTGLEERHHYLIDDAARDIAVLQTGNTSL
                     GVTLLAHLVREAAARLDPEYDIEVVEMHHRNKVDAPSGTALLLGQAAAEGRRIDLARC
                     SERGRDGLTGARGHGKIGFASLRGGTVAGDHDVIFAGPEEMITLSHRAENRMIFARGA
                     VRAALWLIRQKPGRYTMPQVLGLSGSQ"
     gene            2801..3505
                     /locus_tag="VN034_11035"
     CDS             2801..3505
                     /locus_tag="VN034_11035"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007678970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HWV61181.1"
                     /translation="MTPIHNIVILTGAGVSAESGIDTFRDAGGLWEQHRVEDVATPEA
                     FARNPDLVLRFYDMRREAIQTKEPNAAHRALARLDAEWPGELLIVTQNVDDLHERAGA
                     KRLLHMHGEHLNAWCTGCDVRSPWTGPLLDRPGCPACGAAGSLRPDIVWFGEMPYRMD
                     EIYHALNRADLFVSIGTSGAVYPAAGFVREARAAGARTIELNMEPSQGSHWFDEARHG
                     PATALVPAWVEEMLGV"
     gene            complement(3688..4143)
                     /gene="tadA"
                     /locus_tag="VN034_11040"
     CDS             complement(3688..4143)
                     /gene="tadA"
                     /locus_tag="VN034_11040"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWV61182.1"
                     /translation="MPAFPLPEPMRRALDLARIAADWGEVPVGAVVVKDGAVIAEGHN
                     RPRESYDPTAHAEIVAIRMAAAKLGNERLDGCDLYVTLEPCAMCAGAIAHARIARLYY
                     GADDPKGGAVVHGPRTFAQPTIHHRPEIYDGIGEAEAAALLRDFFAARR"
     gene            4284..4577
                     /gene="rpmB"
                     /locus_tag="VN034_11045"
     CDS             4284..4577
                     /gene="rpmB"
                     /locus_tag="VN034_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWV61183.1"
                     /translation="MSRICELTGKGRQVGHNVSHANNKTKRTFLPNLQNVTLLSDALG
                     KGVKLRVSTHGLRSVEHNGGLDNWLLKAGDDQLSATARKLKKEVTKKLAEKAA"
     gene            complement(4615..5622)
                     /locus_tag="VN034_11050"
     CDS             complement(4615..5622)
                     /locus_tag="VN034_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HWV61184.1"
                     /translation="MRRLLFAALIFVALGPMPGTVMRFPKVDLTESAAARPIAFAPAQ
                     KGAVRFVRGWHLVSPHSRFGGFSALARTGANGFQLVGDNGYWTRLTLDSRGAVSDVRI
                     RPLPTPDGRPPRKSMADAEAMFVDPVSGKSWIALEGINQIWRLGADLAAIEARRKLPR
                     WPGNLGPESMVRLTDGRTIVLSEDADDDPRGREGLLYTGDPAEPGPGPIRFFYASDGR
                     GMVSDAAPLPDGRILLVHRRLGFDPVFTTILSVADPADIKADAVVRSRTIGRVPTPLA
                     ENYEGAAVSIEGGRTFLWLVADNNFNVWQRSLLLQFELVDLPPKRTPAKKSGDSKKAA
                     P"
     gene            5787..6194
                     /locus_tag="VN034_11055"
     CDS             5787..6194
                     /locus_tag="VN034_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWV61185.1"
                     /translation="MKKMVTLSIAALMSTATLGLATPAAAQNGYYDRDGYSSYYSGYD
                     RDERRYDRYDRRDYRRDRRDYRNDRRYYRDNRRHYRQCDKGTGGTVIGAIAGGLAGHE
                     IAGRGDRTVGTIIGGAVGALAGRAIDKGNDGCR"
BASE COUNT         1041 a         2036 c         2082 g         1059 t
ORIGIN      
        1 ctgcgcatgc accgccttcg tcaccgccgc gacgacgtcg gggcgcagcg gcgaggcatc
       61 gctcgcgctc gcgtcgggat cggtcttcac cttgatcgcc ggatcggcga tcgccttttc
      121 gagctcggcg cgcaccgttt cgaccggcac gccggggaag atgcggcagt tgatgttcgc
      181 ggtcgcacgc tgcggcagcg cattctcggc atgcccgccc ttgaccagcg tcggcacgca
      241 ggtggtgccg atctggccga tatattcggg gtcggcgcgg atcgcagcaa tggctttcgc
      301 gtccgacggg ttcgcggcaa aggctttcag cgccgcgccg atatcgccgc cgacctggtc
      361 ggcgacgatc ggcatgccga ccttggtcag ctcattctgc tgcggcgtga agcgataggc
      421 gcccaccttg gcgagcgcgg tcgacagctg gacgatcgca ttgctgcccg tggggcgcga
      481 gctgtgcccg ccggggttgg tgacctcgag cgtaaagtcg gcataggtct tctcgcccgc
      541 ctgcagcccg taatattgcg gcttgccgtc ctcggcgatc agcccgccgc ctccgtcgcc
      601 gttcaaggcg aattcggcgc ccttatattt cgccgcgagc gcgcgcgtcg tggtcatcga
      661 ggtttcctcg tcgcccgaga gcagcaggat gatcgaacgc ttcggcttga agccgtcctt
      721 cttgagctgc gccatcgtcg cgaccatcat cgagacgtcg aacttgttgt cttcggaccc
      781 gcggccaaaa atatagccat tctcctcgac cggcacgaag gggtcgcgcg tccagtcctt
      841 ggggtccgcc tcgacgacat ccatatggcc gagcagcacg atcggctttt gcctggtcgt
      901 accggcgagc gtcgcggcga aggtcgcggt ctcgcccatc ggggtgattt cgatatcggc
      961 gtcggcgtaa cccgccgcct tgagcacgcc ggcgtaatag gcggcgagtt cgggcacctt
     1021 gccgcggccc tcgacggtcg ggatcgcgac gctgtccttg aggatcttct tcgccaccgc
     1081 ggggtcggcg ggcttggcgt ggacgggcgc ggcggcgagc agggcggcgg aagcaagcaa
     1141 ggtggaagca aaacgcatat tatcgacccc tttgagtatt tctgttcatc cgttcgggct
     1201 gcgcttgtcg aagccccgtc cttttcccgg ccatgctgga ggaagaacgg cccttcgaca
     1261 agctcagggc aaacggaatt tcatgcggcc gccttccaac ccttcggcgc gggcgtcttg
     1321 ggcttgtaac ggcagaggtc ggcgaccggg cagcgccagc attcgggggt gcgcgccttg
     1381 cagatgtagc ggccgtggag aatcagccag tggtgcgcgc cgacgcggaa gggctgcggc
     1441 gtttccttgt cgagcttctt ctcgaccgcg agcggggttt tgcctggggc gaggccggtg
     1501 cggttcccga cgcggaagat atgcgtgtcg accgcgaagg tctccgcccc gaaggcgcag
     1561 ttcatcacga cattggcggt cttgcgcccg accccgggca gttcgacgag cgtgtcgcgg
     1621 tcggcgggca cctcgccgcc aaaatcgcgg acgaggattt cgctaaggcc gatgacattc
     1681 ttcgccttcg cattgaacag cccgatcgtc ttgatgtgct gcttgagtcc ctcctcgccg
     1741 agatcgacca tttgctgcgg cgtcttcacg tcgcggaaca gcgcccgcgt cgccttgttc
     1801 accccgacgt cggtcgcctg cgccgagagc acgaccgcga cgagcaattg atagatattg
     1861 ccatattcaa gctcggtctc ggggctgggg ttgagctcgg ccagacggcg gtagaattcg
     1921 aaaatatcgg ctttcttcat tgcgagccgc tgagccccag cacctgcggc atcgtgtagc
     1981 gccccggctt ctgacggatc agccacagcg ccgcgcgcac cgcgccgcgc gcgaagatca
     2041 tgcggttttc ggcgcggtgc gacagggtga tcatctcctc cggccccgcg aagatcacgt
     2101 catggtcgcc cgcgacggtg ccgccgcgca ggctcgcgaa accgatcttg ccatgcccgc
     2161 gcgcaccggt gaggccgtcg cggccgcgtt ccgaacatcg ggcgaggtcg atgcgccgcc
     2221 cttcggccgc cgcctggccc aagagcagcg cggtgccgct cggcgcgtcg accttgttcc
     2281 ggtggtgcat ctcgaccacc tcgatatcat attcggggtc gagccgcgcc gccgcctcgc
     2341 gcaccagatg cgcgagcaac gtgacgccga gcgaagtgtt gccggtctgg agcaccgcaa
     2401 tatcccgcgc ggcatcgtcg atcagataat gatggcgttc ctcgagcccc gtcgtgccga
     2461 taacgatcgg tttcttcgcc gcgacgcagg cgtcgagcgt cgcttcaagc gcggcgggcg
     2521 aggagaaatc gacgagcacg tcgctttcgc tcgcgagccg gacgacattg ccgccgcgat
     2581 cgacgccgca gctgcgctgt cccgcctcgg agatcgccgc ggcgagcgcg acgcccatcc
     2641 gcccttcgct gccgataatg ccgatgctgg tcatgttgcg tcccctagat tgccactccc
     2701 tttagccgct cgtgtcgagc gaagtcgaga caccccgcga tccaggccca gaacgatggg
     2761 catctcgact ttgctcgatg cgaacggtta caggggagca atgaccccta tccacaatat
     2821 cgtcatcctg accggcgcgg gcgtgtccgc cgaaagcggc atcgacacgt ttcgcgacgc
     2881 gggcggattg tgggaacagc accgcgtcga ggatgtcgcg acccccgagg cctttgcgcg
     2941 caatcccgac ctggtgctgc gtttctacga catgcggcgc gaggcgatcc agaccaagga
     3001 gcccaatgcg gcgcaccggg cgctggcgcg gctcgacgcc gaatggccgg gcgaactgct
     3061 gatcgtcacg cagaatgtcg acgatctgca cgagcgcgcg ggggcgaaac ggctgctcca
     3121 catgcacggc gagcatctga acgcctggtg caccggatgc gacgtgcgca gcccgtggac
     3181 gggaccgctg ctggaccgcc ccggctgccc cgcatgcggc gccgcgggga gccttcgccc
     3241 cgacatcgtc tggttcggcg agatgccgta ccggatggac gaaatctacc atgcgctgaa
     3301 ccgcgccgac ctgttcgtgt cgatcggcac ctcgggcgcg gtctatcccg ccgcgggttt
     3361 cgtgcgcgag gcgcgcgccg cgggggcgag gacgatcgag ctcaatatgg agccgagcca
     3421 ggggagccac tggttcgacg aggcgcggca cgggccggcg acggcgctgg tgccggcgtg
     3481 ggtggaggag atgctgggcg tgtgatcgtg ctcttcggga cgcggcagtt gcgaccggta
     3541 ggcgacgctt ggtatctcat acctcgtcat tcccgcgcaa gcgggaaccc agtgtggtgt
     3601 cagccgacgc acgctccggg ttcccgcttg cgcgggaatg acgaatgttg ggatgacagc
     3661 tttccaaccc cgaagccgac cataaggtta cctccgcgcc gcgaagaaat ccctcagcag
     3721 cgccgccgct tcggcttcgc cgatcccgtc gtaaatttcc ggccggtggt ggatcgtcgg
     3781 ctgcgcgaag gtgcgcgggc cgtggacgac cgcgccgccc ttggggtcgt cggcgccgta
     3841 atagaggcgc gcgatgcggg cgtgggcgat cgcgcccgcg cacatcgcgc agggttcgag
     3901 cgtgacatag aggtcgcagc catcgagccg ctcgttgccg agcttcgccg ccgccatgcg
     3961 aatcgcgacg atttcggcgt gcgcggtcgg atcgtacgat tcgcgcgggc ggttatggcc
     4021 ttcggcgatg accgcgccgt ccttgacgac caccgcgccg acgggcactt cgccccaatc
     4081 ggcggcgatc cgggcgagat cgagcgcgcg gcgcatcggt tcgggaagag gaaacgcggg
     4141 cataaaagcg ccctattcgc ttgacgaatc tccggcaagc cgataagcgc gcgcctttcc
     4201 cggctcactc cggtcttgtg ctccccgttt tgcgggaagt gtccggacgc cgatccgaaa
     4261 tttttttgac gaggacgaag acgatgtcgc gcatctgcga actgaccggc aagggccgcc
     4321 aggttggcca caatgtcagc cacgccaaca acaagaccaa gcgcaccttc ctgcccaacc
     4381 tgcagaatgt gacgttgctg tcggacgcgc tcggtaaggg cgtgaagctg cgcgtgtcga
     4441 cccacggtct gcgttcggtc gagcataatg gcggcctcga caactggctg ctcaaggccg
     4501 gcgacgacca gctgtcggca acggcgcgca agctcaagaa ggaagtgacg aagaagctgg
     4561 ccgaaaaggc cgcctgagct tccaagcctt tcgaaacgca aaaaggcggc ccggttacgg
     4621 cgccgccttt ttgctgtccc cacttttttt ggccggcgtt cttttgggcg gcagatcgac
     4681 cagttcgaat tgcagcagca ggctgcgctg ccagacgttg aaattattat cggcgacgag
     4741 ccagaggaag gtgcgcccac cttcgatcga tacggccgcg ccctcgtaat tttcggcaag
     4801 cggcgtggga acgcggccga tcgtccgcga tcgcacgacc gcatccgcct tgatgtcggc
     4861 gggatcggcg accgacagga tcgtcgtgaa caccggatcg aagccgagcc gccggtggac
     4921 gagcaggatg cgtccgtcgg gcagcggtgc ggcgtcgctg accatcccgc ggccgtccga
     4981 tgcgtagaag aagcgaatcg gccccggacc gggttcggcg ggatcgccgg tatagagtag
     5041 tccttcgcgc ccgcgcggat cgtcgtcggc gtcttcggaa aggacgatcg tgcggccgtc
     5101 ggtgaggcgc accatcgatt cgggaccgag attcccgggc cagcgcggca gcttgcggcg
     5161 cgcctcgatc gccgcgaggt cggcgcccag ccgccagatc tggttgatcc cctcgagcgc
     5221 gatccagctc ttgccactca cgggatcgac gaacatcgcc tccgcatcgg ccatcgactt
     5281 gcgcggcggg cgtccgtcgg gggtcggcag cggccggatc cggacatcgg aaaccgcccc
     5341 ccgcgaatcg agcgtcaggc gtgtccaata gccattgtcg ccgacgagct ggaaaccgtt
     5401 cgcacccgtc cgcgcgagcg ccgaaaagcc gccgaagcgg ctgtgcgggc taacgaggtg
     5461 ccagccgcgc acgaagcgga ccgcgccctt ttgcgccggc gcgaaggcga tcgggcgcgc
     5521 ggcggcgctt tcggtcaggt cgacctttgg aaagcgcatc acggttccgg gcatcggccc
     5581 gagcgcgacg aagatcaggg cagcgaagag caaacggcgc atcgcggcgg ggttagggtg
     5641 cgcgcatacg aaagggaagc gtttcggcgc ccgaagctga acggcagcca acaatgatgt
     5701 tcaggcttcg ctcaatgcga atcgggcaaa acatgatcat cgaccacagc cccgaagggc
     5761 caagtgacga aagaaggaga aagacgatga aaaaaatggt gactctctcg atcgccgccc
     5821 tgatgtccac cgcgacgctg ggcctggcaa ctcccgccgc ggcgcagaac ggctattacg
     5881 accgcgacgg ctattcgagc tattacagcg gctatgaccg cgacgaacgt cgctatgacc
     5941 gctatgaccg ccgcgactat cgccgcgacc gccgcgatta ccgcaacgat cgccgctatt
     6001 accgcgacaa ccgccgccat tatcgccagt gcgacaaggg caccggcggg accgtgatcg
     6061 gcgcgatcgc gggtggcctc gccggtcatg aaatcgcggg ccgcggcgac cggacggtcg
     6121 gcacgatcat cggcggcgcc gtcggcgccc tcgccggccg cgcgatcgac aaggggaatg
     6181 acggctgccg ttaagggctg tcccgacccc caataatt
//
LOCUS       DATMSU010000411         4315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_139689,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000411 DATMSU010000000
VERSION     DATMSU010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139689"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VN034_11060"
     CDS             <1..828
                     /locus_tag="VN034_11060"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HWV61186.1"
                     /translation="IEAADGRYYIYPTNSGGTGRLHVWTSPDRRHWQKGAELIALDRI
                     GWIGDDGAARHALWAPDMVAANGRHYLYYSVGPQNPTPSRIGVATCVGLEGPCTDSGK
                     PLITGGKGFEAIDPAVFVDPASKTPYLYAGGSAGAKLRVWTLGPDMVTIDREISVETP
                     PHFTEGAFMHERGGTYYLSYSSGSYRHDSYQVHYATAPSPTGPWTYRGKIVESDARFK
                     GPGHHAFLHDPADGRWYIAYHRWEGQTGPGPYKGSRKFIVQPIAYRADGTIVPLEMR"
     gene            complement(830..1561)
                     /locus_tag="VN034_11065"
     CDS             complement(830..1561)
                     /locus_tag="VN034_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWV61187.1"
                     /translation="MSWEQSGLGQDDAPASLGRNLTYGLLDNLGRAIVTGQFDDEAFP
                     TEAELTKRHGVSRSVTREAVKMLTAKGLLSARPRQGTVVQPTSSWNLFDTDVLRWLLE
                     RQFSIELLKQFNQLRVAIEPEAAALAAQFGRDEELKRIGDGLDRMAAAERGEDDTLEA
                     DIAFHVAVLRASNNPFYAQFRDVVSTALRTSIRFTNRIKGRTASVADHAAVRDAIAAH
                     DPDAARSAMRHLIGDVLTLIDDAEK"
     gene            1758..3188
                     /locus_tag="VN034_11070"
     CDS             1758..3188
                     /locus_tag="VN034_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWV61188.1"
                     /translation="MSLAAIASVPAGAALAEGSPPARTAEASKQAERQPNILFILVDD
                     MGFGDLSVMGNKKVQTPNLDRLAREGVLLTKFYDAAPICSSSRAGLLTGRFPADVGFV
                     GITASRARNAEIGQADWLDPKLPTIARLLQRNGYKTAHIGKWHLGGGRDIGDAPWPTT
                     YGFDKSFTTFEGLGPRVLVSDEERFLAQQSDKLGEGPRFWEIKTNLTGLYADMTVDFV
                     AQNSDKPWFVNLWLNDIHDPWAPDDESLSAVMGKGASSDDDRMLASLVKMDKTLGRLV
                     DRLDQMGEMDNTLILFTSDNGPSPLARYHERGETAPGSTEGLRGRKFSLYEGGIRQPL
                     IVYWRGHMKAGTRDEQTVAQGVDLLPTFASILGVKKPAGSVGIDLSPALRGQPVTQRP
                     DLFWAYGKEGRPLLGKPSQPIDVSPRFAVREGDWKLLANDGGADVELYDLARDPMEKQ
                     DLAASEPEVRGRLLAKLKKWLAQLPK"
     gene            3293..4213
                     /locus_tag="VN034_11075"
     CDS             3293..4213
                     /locus_tag="VN034_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006958329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWV61189.1"
                     /translation="MRFIEGGAFTMGSERFYPEERPLRRVKVDSFWIDETPVTNAAFA
                     EFVAATGYRTLAELPPDPKLYPGADLEGVEPGSLLFQKADGLIPLDDPSGWWAFVPRV
                     DWRHPTGPESSIEGLEDHPVVHVAHCDAEAYAKWAGKALPTEAEWEYAARGGIDGQEF
                     AWGDELAPEGAILANYWLGLFPVSTAKADGNYRTMPVGSFPANGYGLSDMIGNVWEWT
                     RDWYAAPQAAKSKSPCCAIANPRGATLAQSYDDATPGARIGRKVMKGGSHLCAVNYCQ
                     RYRPAARHPQHVDSATSHIGFRCVSRRKGE"
BASE COUNT          770 a         1454 c         1390 g          701 t
ORIGIN      
        1 atcgaggccg cggacgggcg ctattatatc tatccgacca actcgggcgg gaccgggcgg
       61 ctgcacgtct ggacctcgcc cgaccggcgc cactggcaaa agggcgccga gttgatcgcg
      121 ctcgaccgga tcggctggat cggcgacgat ggcgcggcac gtcacgcgct gtgggcaccc
      181 gacatggtcg cggcgaacgg ccgccattat ctctattatt cggtcgggcc gcagaatccg
      241 acgccgagcc ggatcggcgt cgcaacctgc gtcggcctcg aaggcccctg caccgacagc
      301 ggcaagccgc tgatcaccgg tggcaagggg ttcgaggcga tcgaccccgc ggtgtttgtc
      361 gatcccgcga gcaagacgcc ctacctctac gccggcggca gcgcgggcgc gaagctgcgc
      421 gtctggacgc tggggcccga catggtgacg atcgaccgcg aaatctcggt cgaaacgccg
      481 ccgcatttca ccgaaggcgc gttcatgcac gaacgcggcg gcacctatta tctctcctat
      541 tcgtcgggca gctatcgcca cgacagctat caggtccatt atgcgaccgc gccctcgccg
      601 acgggccctt ggacctatcg cggcaagatc gtcgagagcg acgcgcgctt caaaggcccc
      661 ggccatcacg ccttcctgca cgatcccgcc gacggccgct ggtatatcgc ctatcaccgc
      721 tgggaaggac agaccggccc cggtccctac aaaggcagcc gcaaattcat cgtgcagccg
      781 atcgcctatc gcgccgacgg cacgatcgtc cccttggaga tgcgctaggc tatttctcgg
      841 cgtcgtcgat caatgtcagc acgtcgccga tcaggtggcg catcgcgctg cgcgccgcgt
      901 cgggatcgtg cgccgcgatc gcgtcgcgga ccgcggcatg gtcggcaacg ctggcggtgc
      961 ggcccttgat ccggttggta aagcggatag acgtgcggag cgcggtgctg acgacgtcgc
     1021 ggaactgggc atagaaggga ttgttcgacg cgcgcaggac ggcgacgtga aaggcgatgt
     1081 cggcttcgag cgtatcgtcc tcgccgcgtt cggccgccgc catgcgatcg agaccgtcac
     1141 cgatccgctt gagctcttcg tcgcgtccga attgcgccgc gagcgccgcc gcctcgggct
     1201 cgatcgcgac acgcagctgg ttgaactgtt tgagcagctc gatcgaaaat tgccgttcga
     1261 gcagccagcg cagcacgtcg gtgtcgaaca ggttccacga cgaggtcggc tgcaccaccg
     1321 tcccctgccg cggccgcgcg ctgagcagcc ccttggcggt cagcatcttc accgcctcgc
     1381 gcgtcaccga ccggctgacg ccgtggcgct tggtcagttc ggcctcggtc ggaaaggctt
     1441 cgtcgtcgaa ctgaccggtc actatcgcgc gccccagatt gtcgagcagg ccataggtca
     1501 gattgcgccc gagcgaagcg ggggcatcat cctggcccaa tcccgattgt tcccacgaca
     1561 cggtgttcgc ctccttttcg agaagccgcg aagcggcctt gcgacaatgt taggctgaac
     1621 agaaaagtag acaagcctaa ttaatacgat aaatatgtat tcattccgca ggaatcggga
     1681 ggttgggaga aaatatgttc aatcggtcat ccaccgcccc gctcccggcc gccaccagat
     1741 ttttcgcgac ggccgcaatg agcttggcgg cgatcgcgtc tgtcccggcc ggggcggcgt
     1801 tggcggaagg atcgcccccc gcgcgcaccg ccgaagccag caagcaagcc gagcgccagc
     1861 ccaatatcct gttcattctc gtcgacgaca tgggctttgg cgacctgtcg gtcatgggca
     1921 acaaaaaggt tcagaccccg aacctcgacc gtctcgcgcg cgaaggcgtg ctgctcacca
     1981 aattctacga cgccgcgccg atctgctctt cgtcgcgcgc gggtttgctg accgggcggt
     2041 ttccggcgga cgtcggcttc gtcggcatca ctgcaagccg cgcacgcaat gccgaaatcg
     2101 ggcaggccga ctggctcgat ccgaagctgc cgacgatcgc gcggctgctc cagcggaacg
     2161 gctacaagac cgcgcatatc ggcaaatggc atttgggcgg cgggcgcgac atcggcgacg
     2221 cgccgtggcc gaccacctat ggtttcgaca agagtttcac gaccttcgaa ggactgggcc
     2281 cgcgcgtact cgtgtcggac gaagagcgtt ttcttgccca gcagtcggac aagctcggcg
     2341 aagggccgcg tttttgggag atcaagacca acctcaccgg cctttacgcc gacatgacgg
     2401 tcgatttcgt cgcgcagaat tcggacaagc cgtggttcgt gaacctctgg ctcaatgaca
     2461 tccacgaccc ttgggcgccc gacgacgaaa gcctgtccgc cgtcatgggc aagggcgcat
     2521 cgtcggacga cgaccggatg ctcgcctcgc tggtcaagat ggacaagacg ctcggccgcc
     2581 tcgtcgaccg cctcgaccag atgggtgaga tggacaatac actgatcctc ttcacgtcgg
     2641 acaacgggcc atcgccgctc gcgcgctatc atgagcgcgg cgagaccgcg ccggggagca
     2701 cggaagggct gcgcggccgc aagttcagcc tttacgaagg cggcatccgc cagccgctga
     2761 tcgtctattg gcgcggccat atgaaggctg ggacgcgcga cgagcagaca gtggcgcagg
     2821 gtgtcgacct gctgccgacc ttcgcgagca tcctcggcgt caagaagccc gcaggcagcg
     2881 tcggcatcga cctgtcgccg gcgctgcgcg gccagccggt cacgcagcgg cccgacctct
     2941 tctgggccta tggcaaggaa gggcggccgc tgttgggcaa accgagccag ccgatcgacg
     3001 tatcgccgcg cttcgcggtg cgcgagggcg actggaagct gcttgcgaac gatggcggcg
     3061 ccgatgtcga gctctatgac ctcgcgcgcg atccgatgga aaagcaggat ctcgcggcca
     3121 gcgagccgga agtgcgcggc cggttgctcg ccaagctcaa gaaatggctt gcccaactgc
     3181 ccaaatgatc gtgcagccga gccccgagcc accaccgaaa cgaaatgatc gcccgaccat
     3241 gaaagccgca tcgcgccgcg ccgcccctgc ccccgcctcg tcactcgacg gcatgcgttt
     3301 catcgagggc ggtgccttca ccatggggtc ggagcgcttc taccccgagg agcgaccgct
     3361 gcgccgcgtc aaggtcgaca gcttctggat cgacgaaacg ccggtgacca acgcggcctt
     3421 cgccgaattc gttgcggcga cgggctatcg cacgctcgcc gagctgccgc ccgatcccaa
     3481 gctttatccg ggcgccgatc tggagggcgt cgagcccggg tcgctgctgt tccaaaaggc
     3541 cgacggcctg atcccgctcg acgatccgtc gggatggtgg gcgttcgttc cccgcgtcga
     3601 ctggcggcac ccgaccggcc ccgaaagctc gatcgaagga ctggaagatc accccgtcgt
     3661 ccatgtcgcg cattgcgatg ccgaggccta tgccaaatgg gctggcaagg cgctgcccac
     3721 cgaggccgaa tgggaatatg ccgcgcgcgg cggcatcgac ggacaggaat ttgcctgggg
     3781 cgacgaactg gcgccggagg gcgccatcct cgccaattac tggctcggcc tgtttcccgt
     3841 ttcgacggcc aaggccgacg gcaattaccg caccatgccg gtcggcagct ttccggccaa
     3901 tggctacggc ctttccgaca tgatcggcaa cgtctgggaa tggacgcgcg actggtatgc
     3961 ggcgccgcag gcggcaaaga gcaaatcgcc ctgctgcgcg atcgccaacc cgcgcggcgc
     4021 gacgctcgcc caaagctatg acgatgcgac gcccggcgcg cgcatcggcc gcaaggtcat
     4081 gaagggcgga tcgcacctct gcgccgtcaa ctattgccag cgctaccgcc cggcggcgcg
     4141 gcatccgcaa catgtcgaca gcgcgacgag ccacatcggc tttcgctgcg tgtcgcgccg
     4201 aaagggagaa tgaacatgcc acgtaccgcg cactggaccc gcagggccct gatcgccctg
     4261 ctgatcaccg cgacgtcggc gtgcagccag gcgtctgcgc cgcgcgccgg cgtgg
//
LOCUS       DATMSU010000412         5497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_92578, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000412 DATMSU010000000
VERSION     DATMSU010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92578"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VN034_11080"
     CDS             complement(<1..235)
                     /locus_tag="VN034_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61190.1"
                     /translation="MRYRDLRGALAASAAICAVAFAAPATARVKTFDIPAQSAATGIP
                     ALAAQADVQILVSAAAVRGRTIRAIKGAMSVDQA"
     gene            complement(327..1307)
                     /locus_tag="VN034_11085"
     CDS             complement(327..1307)
                     /locus_tag="VN034_11085"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWV61191.1"
                     /translation="MSERETARAINDCAAAWVARIDREGENAAVRAELDTWLAGDERR
                     RGAYLRAEATWRMLDRASVLGAGQPSAFDELPVATTISFPRRRLVLSGGAAAAVLLFA
                     AMAGKNMLDAPDERIQTAVGEIRRVPLSDGSLAAVNTQTAIAITMKSRVREITLEKGE
                     AWFQVAKDRARPFVVEAGDVRVRAVGTAFAVRRVAEGVDVQVTEGVVEIWREGDEAAV
                     QRVSAGARAVITPGKAVVPALTANEEMDKALAWRTGQLVFDGETVAEAASEFNRYNVR
                     KIEVTDAALGSQAMIGRFRTNEPEAFARAAATLLDARVILGSDRIILSRN"
     gene            complement(1273..1905)
                     /locus_tag="VN034_11090"
     CDS             complement(1273..1905)
                     /locus_tag="VN034_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV61192.1"
                     /translation="MAFRASLLKEITKRGDVNGQSRARIAAWVGREILPHEGDVRAWL
                     RRALNPEDLEDVIQEAYCQIASLEKIDHIRNARGYFFTTARNIVLMRLRRARIVRIES
                     VTEIESLNIVGDEPSPERVAAARRELDRIRQLIEGLPDRCRRIFELRKIEGVSQREIA
                     ERLGVTEHIVENDVTKGLKLILQAMAEGERRAELMRTEKDERARNSTSDQ"
     gene            2215..2901
                     /locus_tag="VN034_11095"
     CDS             2215..2901
                     /locus_tag="VN034_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWV61193.1"
                     /translation="MGFLSPDLLWPILGATLAGAVIGAEREYRASPAGLRTHILVALS
                     CCLLMLSAVHQMAWLNDAPVEVIRIDPVRMAHGILTGIGFLCGGVIFREGFNVRGLTT
                     AASLWTTSTLGMLFGIGFYELAIFGAGATLTVLAAVSLTERYAPQRRIAHVRATYRRD
                     AAISLSAFGALLASKDLKYITIDQVLDGDKRSFNAIVGGYSEACAEALASVWTADEDV
                     AGFEIRPQQA"
     gene            complement(2925..3536)
                     /locus_tag="VN034_11100"
     CDS             complement(2925..3536)
                     /locus_tag="VN034_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWV61194.1"
                     /translation="MLEIPILYSFRRCPYAMRARMALLVSGMAYAHREVVLRDKPAAM
                     LAASPKGTVPVLVLPDGTVIDESLDIMRWALIRNDPERWLPGSDASLLATFDAAFKYH
                     LDRYKYAVRYDVDPLVHRAEGLAILGELDHRLAERGCISGAVRGFDDIALFPFVRQFA
                     GVDAEWFDANAPQHVRKWLATLIASDLFDQAMARIAPWLDAQD"
     gene            3765..4643
                     /locus_tag="VN034_11105"
     CDS             3765..4643
                     /locus_tag="VN034_11105"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWV61195.1"
                     /translation="MNIDVRSEELIAWIARIGSAALILIVGLWLAFAISKYVRRKAVA
                     HPRIDATLTGFLTMLIRYALIILVLIVVLQQFGVQTTSLVAVLGASALAIGLALQGTL
                     TNVASGIMIAIMRPYHIGDFVEINGKEGTVIDLDLFFTKLRSADERIVLVPNGQAAAN
                     NIVNHTQKGRRRCILVVGIGYEDDIDKALALLADIMTGDDRAIADPPPWFGVRELGDS
                     SVNIEGRVWIPTDKHRVYKSEMTKAVKETFDREGIEIPYPHAVQISKGDVPLRQPPIK
                     PAGKGPQAGRIETSDG"
     gene            4865..>5497
                     /gene="paoA"
                     /locus_tag="VN034_11110"
     CDS             4865..>5497
                     /gene="paoA"
                     /locus_tag="VN034_11110"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HWV61196.1"
                     /translation="MSTSENLPISRRGLLAGGAVSVAATTVAGAPAAEAQPARTKERP
                     PTARVSFEVNGSAHELELDTRTTLLDALREHLRLTGTKKGCDHGQCGACTVIADGRRI
                     NSCLSLAVQHDGDKITTIEGLGLPGKLHPMQQAFVVHDGYQCGYCTPGQICSAVAVLD
                     EIEAGIPSHVTADLSEAPKVTASELRERMSGNICRCGAYSNIIDAIADVAG"
BASE COUNT         1039 a         1831 c         1608 g         1019 t
ORIGIN      
        1 ccgcctgatc gacgctcatc gcgcccttga tcgcgcggat ggtccggccg cgcacggcag
       61 cggcactgac caggatctgg acgtcggcct gcgccgcgag tgcgggaatt ccggtggccg
      121 ccgactgcgc cggaatgtcg aaagtcttga cccgggcagt cgcgggcgcg gcgaaggcca
      181 ctgcacaaat ggccgccgaa gccgcaagcg cgccacgaag atcgcgatat cgcatcgata
      241 catctccccc tctaaacggc ccgattgttc gggctcgtat gacaagatgc gcggctaagg
      301 gatttccgtt ttcgccgacc tgtcattcag ttccgagaca ggatgatccg atcggagccc
      361 aatatgacgc gcgcatccag caatgtggcc gccgctcgcg cgaaagcctc cggctcgttt
      421 gtccgaaagc gcccgatcat ggcttgcgag ccgagcgcgg cgtcggtcac ttcgatcttg
      481 cggacattat agcggttgaa ctcgcttgcc gcctcggcaa ccgtttcgcc gtcgaacacc
      541 agctgcccgg tgcgccacgc gagcgccttg tccatctcct cattcgcggt caacgccggc
      601 acgaccgcct tgccgggggt tatgacggcg cgcgcgccgg ccgaaacacg ctgcaccgca
      661 gcctcatcac cctctcgcca gatttcgaca acgccttcgg tcacctgaac atcgacgccc
      721 tccgcaaccc gccggaccgc gaaagccgtg cccaccgccc gcacccggac gtcccccgct
      781 tcgacgacga agggccgtgc gcgatccttg gcgacctgaa accaagcctc gcccttctcc
      841 agagtgattt cccgaaccct cgatttcatc gtgatcgcga tagccgtctg cgtgttgacg
      901 gcggcgagcg aaccgtcgct tagcgggacg cgcctgatct cgcccaccgc ggtctgtatc
      961 cgctcgtcgg gcgcatcgag catattcttg cccgccattg cggcgaaaag gagcaccgct
     1021 gctgccgctc ctccgctcaa aacaagccgt cttcgcggga aagaaatggt cgtggcgaca
     1081 ggaagctcgt cgaaggcgga cggctgccct gcccccaata cgcttgcgcg gtccagcatg
     1141 cgccaggtgg cttcggcgcg taaataggcg ccgcggcggc gctcgtcgcc ggcgagccaa
     1201 gtgtcgagct cggcacgcac cgccgcattc tcgccctcgc ggtcgatgcg ggcgacccac
     1261 gcggccgcgc aatcattgat cgctcgtgct gtttcgcgct cgctcatctt tttccgttct
     1321 catcagttcg gcgcgccgct cgccctcggc catcgcttgc agaatcagct tcaatccctt
     1381 ggtcacatca ttttcaacga tgtgctcggt cacgccgagc cgctccgcaa tctcccgctg
     1441 ggacactccc tcgatcttgc ggagttcgaa aatacgtcga catcggtcgg gcagcccttc
     1501 gatcagttgg cgaatccggt cgagttcgcg acgggcggcc gccacccgct cgggagacgg
     1561 ttcatcccca acgatgttca ggctttcgat ttccgtaacc gattcgattc tgacgatgcg
     1621 cgcacgccgc agccgcatca ggacgatgtt gcgcgcggtc gtgaaaaaat agccccgggc
     1681 attccgaata tgatcgattt tttccaagct cgcgatctgg caataggctt cctgaatgac
     1741 gtcctcgagg tcttcggggt tgagcgcgcg ccgaagccag gcgcgcacgt cgccctcgtg
     1801 cggcaggatt tcccgcccca cccatgccgc gatacgcgct cgactctgcc cgttcacatc
     1861 accccgcttc gtgatttcct ttaacaacga tgcgcgaaag gccattttcc gtttagcgca
     1921 gcgtctctat cacagtttgg gggtcgggat acttgaggcc ggtgggggac gcttcttgat
     1981 cctgatggca tcaaagccgg catcccagac cgccacctct ggggcagtcg ggatagaaaa
     2041 tacctgaccc cgattggaac gatctgtccg ctggcacgtt gccgaattgc aagtcccgat
     2101 cttcggatcg gacttcagga aagccccgcc gccttgtgct gcggggcttt accttttcca
     2161 tgtcgtcgaa tttctatgcg cggaaccatc cggaccttca cgcggtaaca agccatgggc
     2221 tttctttctc ccgacctcct ctggccgatc ctcggcgcca cgctggccgg cgctgtcatc
     2281 ggcgccgaac gcgaataccg cgcgagcccg gccgggcttc gcacccatat tctcgtcgcg
     2341 ctgtcgtgct gccttttgat gctgtcggcg gttcatcaga tggcatggct caacgacgcg
     2401 ccggtcgaag tgatccgtat cgatcccgtt cgcatggcgc acggtatcct gaccggtatc
     2461 ggcttcctgt gcggcggcgt cattttccgg gaggggttca acgttcgcgg gctgacgacc
     2521 gccgcctcgc tctggacaac atcgacactc ggcatgctgt tcgggatcgg cttttacgag
     2581 ctcgcaatat tcggtgccgg cgccacgctg accgttctcg cggccgtatc gcttaccgag
     2641 cgttatgcgc cgcaacgccg aattgcccat gtgcgcgcca cctaccggcg cgacgcggcc
     2701 ataagcctca gcgcattcgg cgcgctgctc gcaagcaagg acctgaaata cattacgatc
     2761 gaccaggtcc tcgatggcga caaacgcagc ttcaatgcca tcgtcggggg ctatagcgaa
     2821 gcctgcgcgg aagctcttgc aagcgtctgg accgccgacg aagatgtcgc gggattcgag
     2881 atcaggcctc aacaggccta ggacgccgtt cgttaacgcg gctttcagtc ctgcgcatcg
     2941 agccatggcg caatcctggc catggcttga tcgaacaggt ccgacgctat caaggttgcc
     3001 aaccatttgc ggacatgctg cggcgcattc gcatcgaacc attcggcatc gaccccggcg
     3061 aactgccgaa caaacgggaa cagggctatg tcgtcaaatc cccggaccgc gccggaaata
     3121 cagcctcgct ccgccagccg gtgatcgagc tcgccaagta tcgccaatcc ctcggcccga
     3181 tgaaccaaag gatcgacatc atatcgcacc gcatatttat accggtcgag atgatatttg
     3241 aacgcagcgt cgaacgtcgc gagcaaggaa gcgtcggagc caggcagcca ccgctcgggg
     3301 tcgttccgga tcaaggccca gcgcatgata tcgaggctct catcgatcac cgtgccgtca
     3361 gggagaacga gcaccggaac cgtgcccttc ggggacgccg cgagcatcgc ggccggcttg
     3421 tcccgcagca ccacttcgcg atgcgcatag gccatcccgc tcaccagcaa cgccattcgc
     3481 gcgcgcatcg cgtaaggaca gcgacgaaag ctgtagagaa taggaatttc caacattgca
     3541 gggcgtgtag cctatcatgc ccgctcagcc aaaaggcgct cgccattatc ggcggaaacc
     3601 aggcgaggag cgggatcagc agaccggcac cggtcagtca gcgagaattg ggtgcgggct
     3661 cgacaaaccg cattccccgg tagcaccggc cacaggtcgc ccgacctgct ggtcgcaacc
     3721 gcatcacgcc tcgcccgttt atccagcgag ggcaggagat gccgatgaat atcgatgttc
     3781 gttccgaaga gctcatcgct tggatcgccc gtatcggatc ggctgccctg atcctcatcg
     3841 tcggcctgtg gctcgccttc gccatctcca aatatgtgcg caggaaggcc gtcgctcatc
     3901 cgcggatcga cgcgacgctc acgggctttc tgacgatgct gatccgctac gcgctcatca
     3961 ttctcgtcct catcgtcgtc ttgcagcagt tcggcgtaca gaccaccagc ctcgtcgcgg
     4021 tgctcggcgc gagcgcgctt gcgatcggcc tcgcgctcca gggcacactc accaatgtcg
     4081 cgtcggggat catgatcgcg atcatgcgcc cctatcacat cggcgatttc gtcgagatca
     4141 acggcaagga agggacggta atcgacctcg atctgttctt caccaaactg cgatcggccg
     4201 acgagcgcat cgtgctggtg cccaacggtc aggcggcagc caacaatatc gtcaaccaca
     4261 cccagaaagg gaggcgccgc tgcatccttg tcgtcgggat cggttatgaa gacgacatcg
     4321 acaaggccct cgcgcttctc gccgacataa tgactggaga cgaccgcgca attgccgacc
     4381 ctccgccatg gttcggtgtt cgcgagcttg gtgattcttc ggtcaatatc gaggggaggg
     4441 tgtggattcc gaccgacaag caccgcgttt acaagtcgga gatgacaaag gctgtgaaag
     4501 agaccttcga tcgcgagggg atcgaaatcc cttatcccca cgcggttcag atcagcaaag
     4561 gcgatgtgcc gcttcgccag cctccgatca agccagccgg gaaagggccg caggccggtc
     4621 gcatcgaaac gtcggacgga tagtgggccg cacttccgcc atcggcaggc gaatttccga
     4681 gacgcgcttg cgacgcgaac cggcaagcca cggctcgccg attgaggaaa cctcacagct
     4741 tatgctatcc ttgcggcgac ccgaaatcgt ctggggcgtg gcaacgcatc gtcccgcttt
     4801 gcattcattt gtaagaacaa gcacggcgga gccgccgcgc ggaaacaaga tgatgggaac
     4861 tgccatgtcg acatccgaga atctcccgat ctcgcgtcgc ggactgctcg ccggcggcgc
     4921 cgtttcggtc gccgccacaa ccgtcgcggg cgcgccggcc gccgaagcgc aacccgcaag
     4981 aacgaaggag cgaccgccga ccgcacgtgt cagcttcgag gtcaacggct ccgcgcatga
     5041 gctcgaactc gacacgcgca cgacgttgct cgatgcgctt cgcgaacatc tccgcctgac
     5101 gggaaccaag aagggctgcg atcatggtca atgcggcgcc tgcaccgtca tcgccgacgg
     5161 ccgccggatc aacagctgcc tttcgctcgc cgtgcagcat gacggcgaca agatcacgac
     5221 gatcgagggg ctgggccttc cgggcaagct ccatccgatg cagcaagcct tcgtcgtcca
     5281 cgacggctat caatgcggct attgcacccc cggccagatt tgttcggcgg tcgcggtcct
     5341 cgacgagatc gaagcaggca tccccagcca tgtcacggcg gatttgagcg aagcgcccaa
     5401 ggtaacggca agcgaacttc gcgagcggat gagcggcaac atctgccgct gcggcgccta
     5461 ttcgaacatc atcgacgcga tcgccgatgt ggcggga
//
LOCUS       DATMSU010000413        15065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_92601, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000413 DATMSU010000000
VERSION     DATMSU010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 15065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15065
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92601"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..356)
                     /locus_tag="VN034_11115"
     CDS             complement(96..356)
                     /locus_tag="VN034_11115"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61197.1"
                     /translation="MDFTITEDQATLTTGVRDYLAGVHTPELLRALDASNSRRSAEVW
                     RGLVEMGLPGLLVPEAQGGLGLSLLDGVLIAAERLVKLFRAH"
     gene            complement(363..1514)
                     /locus_tag="VN034_11120"
     CDS             complement(363..1514)
                     /locus_tag="VN034_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61198.1"
                     /translation="MDLAYTTEQQAFRAEVRAWMEANVPRDPLVTLEREDGYHQHVEW
                     ERKLASGNWGMVTWPEAYGGRGLDLIQWLIFEEEYYRAGAPLRVNQNGIFLLGPTVME
                     FGTDEQKARFLTPMAKGEVIWAQAWSEPNAGSDMAAITSKAVRDGDHYVLSGQKTWSS
                     RASFADWGFGLFRTDPESKRHKGLSFILFDLNAPGVTRRPIRQLHGDPGFAELFFDEV
                     RVPVENRIAGEGEGWNVAMATAGFERGLMLRSPARFQAAAERLVKLFRAHEAKAAPGA
                     REQVMQAWMDAQAYAYNTYAVASKIMAGGHIGAEASLNKIFWSELDRAAHRTAMQLLG
                     TSAELRHLDGGALNMWLEGYIFSLSGPIYAGSNEIQRNIIAERLLGLPR"
     gene            complement(1514..2269)
                     /locus_tag="VN034_11125"
     CDS             complement(1514..2269)
                     /locus_tag="VN034_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HWV61199.1"
                     /translation="MPIHTEIKDRIAEIVFDVPPVNAFDSTTWNSLPAIIRDAGSNPD
                     VNVVLIRAAESARGFCGGVDIKEMQAHPERITLLNRGNYLTFKAVRDCEVPVVVAVHK
                     FVIGGGIGICGASDTIIAADDAFFSLPEVDRGAMGGASHLSRMLPLHKVRAAFFTGGN
                     IPVAEAYRLGAVEKVVPRDALVDEARAFAAIIAGKSRKALVIAKEALNGLEARDVDRG
                     YRWEQGFTLEMYMHEDSQKSRDAFVETGKAAEF"
     gene            complement(2286..3362)
                     /locus_tag="VN034_11130"
     CDS             complement(2286..3362)
                     /locus_tag="VN034_11130"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWV61200.1"
                     /translation="MADPLATLLTERLGCRLPVIQTAMGWIATPSLVIASSEAGAFGF
                     LAGAVMQPAELEAAILAVKAGTKQPFGVNFHMFQPGAAEIVEIILKHRDQIRAVSFGR
                     GPDAKMIGRFKDAGILCVPTVGAAKHAQKMVQLGVDMVTVQGGEGGGHTGSVASTILL
                     PQVLDAVKVPVIAAGGFGDGRGLAAALAYGAVGIAMGTRFLMTRESPIPDLTKARYVK
                     AGTDDILVSTKVDGMPQRMIKNALLDRIERSSGLGIWRRAIESGLQMKRQTGASFAEL
                     FRAAKGMTSHGGLTLPQAMMAASAPMLIQKAVVEGDPDHGLMATGLVAGRLEDLPSCA
                     DLIASIETEARARLAALSPSSPAS"
     gene            complement(3362..4285)
                     /locus_tag="VN034_11135"
     CDS             complement(3362..4285)
                     /locus_tag="VN034_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWV61201.1"
                     /translation="MTDQNDGLVPPVDETPVYETDTPVLYEAADGIAWVTLNRPQYHN
                     VQNSQMTYALDDAFFRAVDDDAVKVIVLKSDAKHFSAGHDIGTPGRDFHSPVERRLMW
                     YDHSTKAGAEKAYAREHEQYLGMCKRWRDIPKPTIAQVHGGCIAGGLMLAWVCDLIIT
                     SDDAFFQDPVLQMGFPGLEYFAHCHELNVRLAKEFLFLGERMSASRAREAGMVNRVVP
                     RAELADEVKRVAERIATQPRLALQLAKQACNHMETLAGKNAGIDAAFGYHHFAHANNS
                     LVKGDYIAGFDGKKMAEANKKDGSQKDQGAS"
     gene            complement(4289..5089)
                     /locus_tag="VN034_11140"
     CDS             complement(4289..5089)
                     /locus_tag="VN034_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HWV61202.1"
                     /translation="MTTAPDFTLCEQLIVAASEAWRGNGELVATGIGPAPRLAAGLAK
                     LTHTPELMMTDGEAYLVEDPVPLGPRGYDDRKAAGYLPFSRFFDAAVWSGKRHAMVTP
                     SQIDRFGQTNISYLGGTYDQPKVQMLGARGFPGNSICHINSMFVPAHSKRVFVEGEVD
                     MVSSVGYNPARRTPGVDFSRVDLRLIVTDLCVMDFGGTDNAIRVVMLHPGVTFDQVQE
                     ATGFALEAASEITETPAPTAEQLAIIARLDPHGLRAGVIKDNPAGKRS"
     gene            complement(5103..5978)
                     /locus_tag="VN034_11145"
     CDS             complement(5103..5978)
                     /locus_tag="VN034_11145"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWV61203.1"
                     /translation="MNKQLTIEDFVASLKSGMTIGIAGWGPRRKPMALVRAILRSDLT
                     DLTIVAYGGPDVGMLCAAGKVKKLIFGFVSMDAIPLEPYFRKAREAGTLDILELDEGM
                     LQWGLKAAAFRLPFLPTHVGLGTDVMTHGGFKTVQSPYDDGEVLLAMPALKLDAALLH
                     VHRADKLGNIQALGPDTYYDEWFARAADKTFVSAEEIVERMDHSYPDDARANIVERCF
                     IHGVIAAPLGAHPTSMPPSYGWDMKHLKTYAASAREDGGWDAYLADTIGADEAGYLGR
                     VGGKDAVAALPLPIF"
     gene            6130..6894
                     /locus_tag="VN034_11150"
     CDS             6130..6894
                     /locus_tag="VN034_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61204.1"
                     /translation="MADEKQRVALVTGGSRGIGRAIAERLLKEGMRVVICGRNAPEDL
                     PRRHDAVAEFEACDVRSADEVNAMIDRIAERHGRLDLAVNNAGGSPEADAATASPRFS
                     ERVLALNLLGPLHVAQAANRIMQAQAEGGGIVNIASVSGVRPSPGTAVYGAAKAGLLS
                     LTESLAMEWGPKVRVNAVVVGLVATEAAADHYGGAEGMKRIDTMLPLGRMAKGADIAG
                     VVSFLASADAAYVSGARIAVHGGGERPVFLTLAEGG"
     gene            complement(7005..7862)
                     /locus_tag="VN034_11155"
     CDS             complement(7005..7862)
                     /locus_tag="VN034_11155"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HWV61205.1"
                     /translation="MKTTFLALLLLAPAPAMAWSETIAARDTTEAPAKREIALAGGDN
                     FRDVGGYRTKDGHAVAWGKLYRSASVGRYTAEDQALIRKLSVGSIIDLRSTRERQGDK
                     TPWMQDADVGYWTRDYDLSGGNLGALFAKGAKLDAATMRDVMIHGYRGFPKEQAASYR
                     VLFDRLLNSDKAVIVNCTAGKDRTGVGVALVLSALGVPYETIMEDYLLSNTMLDAEAL
                     QADAGMKAAMTALPPDVAKPLLGVERAYLDAAFEQINKDYGSVEAYLDRELGVDAAAI
                     QRLRQQMLS"
     gene            complement(7901..10135)
                     /locus_tag="VN034_11160"
     CDS             complement(7901..10135)
                     /locus_tag="VN034_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61206.1"
                     /translation="MSNGGLHLLRIALAASAATWLATPAAAQESTGDQTIGVTDIVVT
                     AQKREENLQDTPISMVALSSETLEEKGIGSLSDLFTGAVPSIRIAPFVGRVSAVSIGM
                     RGLVPVDATQVSRDPTVGIYIDSVYLGRVSGLGMELADIERIEVLRGPQGTLFGRNTI
                     GGAISVVSKRPTGEASVDFKAGIGNYRGRSLAAHVNLPEAAGISVKVDGVFEGRDGLV
                     KNPLGGDALDYGEIKRYGFKISALWRPADNLSLLYAYDLSRDKSTGYYHYITASDQSP
                     AVTPSFITLDTGRVRTGRFGFPVFRNPQEAKGHSITAEWDVSDALTVRSISAWRNLDS
                     TQLDTDEGSNTTWGPNRRFGRLSYANVKQSQFSQEVQLVGDVGELKFVLGAYYFKEKG
                     QDEAIVYSLGTLNQALTGVNFFPEPSPDGGAGNLPDRAALVDVKSKALFGQATWSPEA
                     LRGLHITAGARYTDDHKDGRLLSIRGVDPNLAFVFDSSRVDPMATIAYDFSEDINAYV
                     KWSRAYRAGGANTRSVILRTFGEEELTAWEAGVKADLFDRRLRVNVAAYMSRLSNQQV
                     DFLNPAFISNTETVNAPEKRKIKGVEADITLVPARGLSLSANYVYTDAPPTPVRNIFT
                     GEIEQTSSAFTPKHAASFSADYVFPAFSFGQLRAYVGLDTAGNYIPNGTVDVKADKAL
                     LLNGRLTLGDIDLGGGTLEVALWGKNLTNATYNLFDFRIPRRTTATLYNEPRTYGIEA
                     RVRF"
     gene            10268..10894
                     /locus_tag="VN034_11165"
     CDS             10268..10894
                     /locus_tag="VN034_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61207.1"
                     /translation="MTIPMPATRKERADATREAILHEAERVFAATGFRAARLEDIADA
                     VGIRRPSLLHHFSTKKMLYDAVEQRIFENMHAVCEARTAGVEDPTARLLAHLDAWLDF
                     FIDRPPAARILQRLVADTAVRGDNPVEFSYVALEDMDRTLAYGIARGAFVPLSTMTFV
                     NGVAAGILFFVCNSRQVGEQRRYDPSDPSDKAVFRTLLHKLARTAVLP"
     gene            complement(10909..11526)
                     /locus_tag="VN034_11170"
     CDS             complement(10909..11526)
                     /locus_tag="VN034_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWV61208.1"
                     /translation="MKPIRHVRGEATRQAILEAAETVFAEVGFTTARLEDVAATVGIR
                     RPSIVYYFSNKQELYDAVEEDIFAAMHREALSRIAQQGKPIDKLLALFDGWLDFLVAR
                     PSAARIIQRLVADVTPRGGDPTQFSNVLLRDVETVVNEGVAAGDFRPLPPMHVVNAVG
                     SGSLFYVCNSKQLGSNRSYDAADPKELESFRELLHLLTRAAVAPA"
     gene            11697..13877
                     /locus_tag="VN034_11175"
     CDS             11697..13877
                     /locus_tag="VN034_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61209.1"
                     /translation="MKKVTGPQARLLLSTLAIAAAQPALAQTAATATDESQGLGEIVV
                     TAQKRAESLQDTPISIAAFTAEELETKAITGLTDLRANVPNLQLTPHPNSAATTQIFM
                     RGVGLSDDQITQDGGVAVYMDGVYIARSQGQALEVAELERVEVLRGPQGTLYGRNATG
                     GAINFITKKPELEDFGFKGQVTLGNFNNRRFKAAVNVPVGNTLAARFSYANIRQDGFV
                     KNPGTGVKRWGDKDRQAMRADVLWQPSDAFSLRYGYDRSEIEDTPIYVVDSPLHPLKA
                     PRPKAGSPLVRDLLPNDVTSQGHSLIAEWEAADALTVRSITGYRKLSNFQNQDYLTGA
                     TGPDPLQKNSSLTKQDQWSQELQLVGDLLDRQIEYVAGVYYFSEDGSNFSNSYSPPRR
                     TRSFTTATIANESWAVFGQATYSPEWLDRRLHLTVGARQSWDKREATLRRETQVNEGP
                     IVPVPGFGDGSRKFTDFSPSFVIAYDVADDVNVYAKAVKGYKAGGYNIRASSIPRFNE
                     GFGSETLWSYELGLKSQWLDDRLRLNVAAFQSKYKDIQINVQSDPTNVRITDVLNAGK
                     ATVRGVEVDLTLAPTRDLRFGIHYGYLDAQYDEIIDGAGNDISADYRFTNAPKNTLAF
                     DANYTLPDLPVGALSANVNYTMQSDKFTGSTIAGGKFITGDYGLLNARLTLAEIPGVE
                     GVRVGLWGHNLTDTEYYIMQFNIGRPGALFGEPRTYGVDLTVEF"
     gene            13882..14925
                     /locus_tag="VN034_11180"
     CDS             13882..14925
                     /locus_tag="VN034_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWV61210.1"
                     /translation="MNRARIPAIALGALALSLSGAAFGAGQGAKDYRPGAADVARTQK
                     DFAGGASEFRFVVVGDRTGKHRPGVFEDAMDKVERLRPDFVINIGDLIEGNTEDPAQL
                     DKEWNEVNVAIDRLSIPFFYVPGNHDLTNEVQRTEWRKRLGADYYSFTYKNALFLVLN
                     TEDPPQPKIARMQLFQQYGGEAMKTVFEALQGDPAKAEALFAGDPKLGELAAKIRGSE
                     NVAFSADQVKMVRDALAKYPKARWTFVLMHRPAWKVDSPAFREIEQMLKGRPHTVLAG
                     HYHKYAYEAHGGTDYIQLGTTGGIPGGAADDPAVVDHVMWVSVADGAPRVSNLKLDGL
                     FAKQGPAAIVRPD"
BASE COUNT         2621 a         4841 c         4960 g         2643 t
ORIGIN      
        1 atcttcgagg ctacggcata ggtgttgtaa gcataggctt gcgcgtccat ccaagcctgc
       61 atcacctgct cgcgcgcgcc gggcgcggcc ttggcctaat gcgcgcggaa cagtttgacg
      121 aggcgttcgg cggcgatcag cacgccgtcg agcagcgaca ggccaagccc gccctgcgct
      181 tcgggaacga gcaggcccgg gaggcccatc tcgacgagcc cccgccagac ctcggccgag
      241 cggcggctgt tgctcgcatc gagcgcgcgc agcagctcgg gcgtgtgaac tcccgccaga
      301 tagtcgcgca cgcctgtcgt cagcgtcgcc tgatcttcgg tgatggtgaa atccatgcct
      361 gcttacctcg gcaggccgag caggcgctcg gcgatgatgt tgcgctggat ctcgttcgaa
      421 ccggcgtaga tcgggcccga gagcgagaag atatagcctt cgagccacat gttgagcgcg
      481 ccgccgtcga ggtggcgcag ctcggccgag gtgccgagga gttgcatcgc ggtgcgatgc
      541 gcggcgcggt cgagttcgga ccagaagatc ttgttgaggc tcgcctcggc gccgatatgc
      601 ccgcccgcca tgatcttcga ggctacggca taggtgttgt aagcataggc ttgcgcgtcc
      661 atccaagcct gcatcacctg ctcgcgcgcg ccgggcgcgg ccttggcctc atgcgcgcgg
      721 aacagtttga cgaggcgttc ggcggcggcc tgaaagcgcg cgggcgagcg cagcatcagc
      781 ccgcgctcga aaccggcggt cgccatcgcg acgttccagc cctcgccttc gcccgcgatg
      841 cggttctcga ccggcacgcg cacttcgtcg aagaagagtt cggcaaagcc cggatcgccg
      901 tggagctggc ggatcggccg acgcgtcacg ccgggggcat tgagatcgaa caggatgaag
      961 ctcaatccct tgtggcgctt cgattcggga tcggtgcgga acaggccaaa gccccagtcg
     1021 gcgaagctcg cgcggctcga ccaggtcttc tggcccgaga gcacataatg gtcgccgtcg
     1081 cggaccgctt tcgacgtgat cgcggccatg tccgaccccg cattgggttc ggaccaggcc
     1141 tgcgcccaga tgacttcgcc cttcgccatc ggggtcagga agcgcgcctt ctgttcgtcg
     1201 gtgccgaatt ccatcaccgt cgggccgagc aggaagatgc cgttctggtt gacgcgcagc
     1261 ggcgcccccg cgcgataata ttcctcttcg aagatcagcc actggatcag gtcgagcccg
     1321 cgtccgccat aggcttcggg ccaggtcacc atgccccagt tgccgctcgc gagctttcgc
     1381 tcccattcga catgctggtg gtagccgtcc tcgcgctcca aggtgacgag cgggtctctg
     1441 ggaacgttcg cttccatcca cgcgcgcact tcggcgcgga aggcctgttg ttcggttgtg
     1501 taggcgaggt ccatcagaat tcggccgcct tgcccgtctc gacaaaggcg tcgcgcgatt
     1561 tctggctgtc ctcgtgcatg tacatttcga gggtgaagcc ctgttcccag cgatagccgc
     1621 gatcgacgtc gcgtgcttcg agcccgttga gcgcttcctt ggcgatcacc agcgccttgc
     1681 gcgatttgcc cgcgatgatc gcggcaaagg cgcgggcctc gtcgaccagc gcgtcacgcg
     1741 ggacgacctt ttcgaccgcg ccgagccggt acgcttccgc gaccgggatg ttgccgccgg
     1801 tgaagaaggc cgcgcggacc ttgtgcagcg gcagcatccg cgagaggtgc gaggcgccgc
     1861 ccatcgcgcc gcgatcgacc tcgggcagcg agaagaaggc gtcgtcggcg gcgatgatcg
     1921 tgtcgctcgc accgcagatc ccgatgccgc cgccgatcac gaatttatgc accgcaacga
     1981 cgacgggtac ctcgcaatcg cgcaccgcct tgaaggtcag gtaattgccg cggttgagca
     2041 gggtgatgcg ctcagggtgc gcctgcattt ccttgatgtc gacgccgccg cagaaaccgc
     2101 gcgcgctttc ggcggcacgt atgaggacga cgttgacgtc gggattgctg ccggcgtcgc
     2161 ggatgatcgc gggcaggctg ttccacgtcg tactgtcgaa cgcgttgacc ggcgggacgt
     2221 cgaagacgat ctcggcgatg cggtccttga tttcggtatg gatcggcatg ggtcagaccc
     2281 ttgttttaag aagctggcga agaaggggaa agcgcggcga ggcgcgcgcg cgcttcggtt
     2341 tcgatggatg cgatgaggtc agcgcagctt ggcaggtctt cgagccgccc ggcgacgagg
     2401 ccggtcgcca tcaggccgtg atcggggtcg ccctcgacca ccgccttctg gatcagcatc
     2461 ggggcggagg cggccatcat cgcctgcggc aaggtgagcc cgccatggct ggtcatgccc
     2521 ttcgcggcgc ggaacagctc ggcgaacgag gcgcccgtct ggcgcttcat ctgcaatccg
     2581 ctttcgatcg cgcggcgcca gatgccgagg cccgacgaac gctcgatgcg gtcgagcaac
     2641 gcatttttga tcatccgttg cggcatgccg tcgactttgg tcgacacgag aatatcgtcg
     2701 gtgcccgctt tgacgtagcg cgccttggtg aggtcgggga tcgggctttc gcgcgtcatc
     2761 aggaagcgcg tgcccatcgc gatgccgacc gcgccatagg ccagcgccgc cgcgagcccg
     2821 cgcccgtcgc cgaagccgcc cgcggcgatc accggcacct tcaccgcgtc gagcacctgc
     2881 ggcagcagga tcgtcgaggc gaccgaaccg gtatgcccgc cgccttcgcc gccctgcacg
     2941 gtgaccatgt caacgccaag ctgcaccatc ttctgcgcat gcttggccgc gccgacggtc
     3001 ggcacgcaga ggatgcccgc atccttgaag cgcccgatca tcttcgcgtc gggaccgcgc
     3061 ccgaagctga ccgcgcgtat ctggtcgcgg tgcttcagga tgatctcgac gatctccgcc
     3121 gcgccgggct ggaacatgtg gaaattgacg ccgaagggct gctttgtgcc cgccttgacc
     3181 gcaaggatcg cggcctcaag ctcggcgggc tgcatcaccg cgcccgcgag aaagccgaac
     3241 gcgcccgcct cgctcgacgc gatcaccagc gacggcgtcg cgatccagcc catcgcggtc
     3301 tggatcaccg gcagccggca gccgagcctt tcggtcaaca gggtggcgag cggatcggcc
     3361 atcagctcgc gccttgatct ttctgggagc cgtccttctt gttcgcctcg gccatcttct
     3421 tgccgtcgaa cccggcgata tagtcgccct tgacgaggct gttgttggcg tgggcgaagt
     3481 gatgatagcc gaacgccgcg tcgatcccgg cattcttgcc cgccagcgtc tccatatggt
     3541 tgcacgcctg cttcgcgagc tgcagcgcga ggcgcggctg cgtcgcgatc cgctcggcga
     3601 cgcgcttgac ctcatcggcc agttcggcgc gcggtacgac gcggttgacc atcccggctt
     3661 cgcgtgcgcg gcttgcgctc atccgttcgc cgaggaacag aaattccttc gccagccgga
     3721 cattgagttc gtggcaatgc gcgaaatatt cgaggcccgg aaagcccatt tgcagcaccg
     3781 gatcctgaaa gaaggcgtcg tccgaagtga taatgaggtc gcacacccac gcgagcatca
     3841 gcccgccggc gatgcagccg ccatggacct gcgcgatggt cggcttggga atgtcccgcc
     3901 accttttgca catgcccaga tattgttcat gctcgcgggc ataggccttt tcggcgcccg
     3961 ccttggtcga atgatcgtac cacatcagcc ggcgctcgac aggcgaatga aagtcgcgcc
     4021 ccggcgtgcc gatgtcgtgg ccggcgctga aatgcttggc gtccgatttc aggacgatga
     4081 ccttgaccgc atcgtcgtcg accgcgcgaa agaaggcgtc gtcgagcgca taggtcatct
     4141 ggctgttctg gacattgtga tattgcgggc ggttgagcgt cacccacgca atgccgtcgg
     4201 ccgcctcata gaggaccggc gtgtcggttt cgtagaccgg ggtttcgtcg accggcggga
     4261 ccagcccgtc attctgatcg gtcatggctc agctcctctt gcctgcggga ttgtccttga
     4321 tgacgcccgc gcgcaggcca tgcgggtcga gccgcgcgat gatcgcgagc tgttcggcgg
     4381 tcggggcggg tgtttccgta atctctgatg cggcttcgag cgcgaagccg gtcgcttcct
     4441 gaacctgatc gaaggtcacg ccggggtgga gcatgacgac gcggatcgca ttgtcggtgc
     4501 cgccgaaatc catgacgcag aggtcggtga cgatcaggcg caggtcgacg cggctgaaat
     4561 cgacgccggg ggtacggcgc gccgggttat agccgacgct cgacaccata tcgacctcgc
     4621 cctcgacgaa gacgcgcttc gaatgcgccg gcacgaacat cgaattgatg tggcagatgc
     4681 tgttgcccgg aaagccgcgc gcgccgagca tctgcacctt cggctggtca taggtgccgc
     4741 cgaggtagga gatgttcgtc tgaccgaaac ggtcgatctg gctcggcgtc accatcgcgt
     4801 ggcgtttgcc cgaccacacc gcggcgtcga agaagcgcga gaaaggcagg tagcccgcgg
     4861 ccttgcggtc gtcgtaaccg cgcggaccga gcggcaccgg atcctcgacc agatacgcct
     4921 cgccatcggt catcatcagt tcgggggtgt gcgtcagctt ggcaaggccg gcggcgaggc
     4981 gcggcgcggg gccgatgccg gtcgcgacga gttcgccatt gccgcgccac gcctcggacg
     5041 cggcgacgat gagctgttcg caaagggtga agtcgggtgc ggtggtcatg atgtcggccg
     5101 cctcagaaaa tgggaagggg gagggcggcg acggcgtcct tgccgccgac gcggcccaga
     5161 tagcccgcct cgtcggcgcc gatggtgtcg gcgagatagg cgtcccagcc gccgtcctcg
     5221 cgcgcgctcg cggcataggt tttgaggtgc ttcatgtccc agccgtagga cggcggcatc
     5281 gaggtcggat gcgcgcccag cggcgctgca atcacgccgt gaatgaagca gcgctcgacg
     5341 atgttggcgc gtgcgtcgtc gggatagctg tggtccatcc gctcgacgat ttcctcggcc
     5401 gaaacgaagg tcttgtccgc ggcgcgcgcg aaccattcgt cataataggt gtcgggcccg
     5461 agcgcctgga tattgccgag cttgtcggcg cggtggacgt ggagcagcgc ggcgtcgagc
     5521 ttgagcgcgg gcatcgcgag cagcacctcg ccgtcgtcgt agggcgactg gacggtcttg
     5581 aacccgccgt gcgtcatcac gtcggtgccg agcccaacgt gcgtcggcag gaagggcagg
     5641 cggaacgccg cggccttcag cccccattgc aacatcccct cgtcgagttc gaggatgtcg
     5701 agcgtccccg cctcgcgcgc cttgcgaaaa tagggttcga gcgggatcgc gtccatcgag
     5761 acgaaaccga agatgagctt cttcaccttg cccgcggcgc agagcatgcc gacgtcgggg
     5821 ccgccatagg cgacgatcgt caggtcggtg aggtccgaac gcaggatcgc gcggaccagc
     5881 gccatcggct tgcggcgcgg cccccagccg gcaattccga tcgtcatccc gctcttcagc
     5941 gaggcgacga aatcttcgat ggtcaattgc ttgttcaatc cactctccag cccacaggcg
     6001 ccgggcacga gggcgcccgc gatgttgcga gatggatagg cgcgctggcc gttcgcccga
     6061 taccaccata tggagagaga ctttcccgcc gcccgatcag agagaggtgg gcgagaaacg
     6121 gagatcgaca tggcggacga aaaacagcgc gtggcgctcg tgaccggcgg cagccgcgga
     6181 atcggccgcg cgattgcgga gcggcttctg aaagagggca tgcgcgtcgt gatctgcggc
     6241 cgcaatgccc ccgaggacct gccgcgccgt catgatgcgg tggccgagtt cgaggcgtgc
     6301 gacgtccgtt cggcggacga ggtcaacgcg atgatcgacc gcatcgccga acgtcacggc
     6361 cggctcgacc tcgccgtgaa caatgccggg ggatcacccg aagccgatgc ggcaaccgct
     6421 tcgccgcgct tctccgaacg ggtgctggcg ctcaacctgc tggggccgct gcacgtggcg
     6481 caggccgcca accgcatcat gcaggcgcag gccgagggcg gcggcatcgt caatatcgcg
     6541 agcgtgtcgg gggtgcgtcc ttcgccgggc accgccgtct atggcgcggc caaggcgggg
     6601 ctgctcagcc tcaccgaatc gcttgcgatg gaatgggggc cgaaggtgcg cgtcaatgcg
     6661 gtggtcgtcg gcttggtggc gaccgaagcg gcggccgacc attatggcgg cgcggaagga
     6721 atgaagcgca tcgacacgat gctgccgctc ggccgcatgg cgaagggcgc ggacattgcg
     6781 ggcgtggtga gctttctcgc gtcggctgac gccgcctatg tcagcggcgc gcgtatcgcg
     6841 gtgcacggcg gcggcgagcg gccggtgttt ctcacgctgg cggagggtgg gtaacgcgcg
     6901 acctctccac cgtttgccct gagcctgtcg aagggccgtc ctttcttcgc agcggaagag
     6961 gaagaacggt gcttcgacaa gctcagcacg aacgggggca gggttcagct cagcatctgc
     7021 tggcgcagcc gctggatcgc cgccgcgtcg accccgagtt cgcggtccag ataggcttcg
     7081 acgctgccat aatccttgtt gatctgctcg aacgcggcgt cgagataggc gcgctcgacc
     7141 cccagcaggg gcttggcgac gtcggggggc agcgccgtca tcgccgcctt catccccgcg
     7201 tcggcctgca acgcctcggc gtcgagcatc gtgttgctca agagataatc ctccatgatc
     7261 gtctcatagg gcacgccgag cgcgctcagc accagcgcga cgccgacgcc ggtgcggtcc
     7321 ttgcccgcgg tacaattgac gatcaccgcc ttgtcggaat tcagcagccg gtcgaaaagc
     7381 acgcggtagg acgcggcttg ctccttgggg aagccgcgat agccgtggat catcacgtcg
     7441 cgcatcgtcg ccgcatcgag cttggcgccc ttggcgaaca gcgcacccag attgccgccc
     7501 gacaggtcat agtcgcgcgt ccagtagccg acgtccgcat cctgcatcca cggcgtcttg
     7561 tcgccctggc gttcgcgcgt cgagcgcaga tcgatgatcg accccaccga caatttgcgg
     7621 atcaaggcct gatcctcggc ggtatagcgc ccgaccgacg ccgagcgata gagcttgccc
     7681 cacgccaccg catggccgtc cttggtgcgg tagccgccga cgtcgcggaa attatcgccg
     7741 ccggcgagcg cgatttcgcg cttcgccggc gcttcggtgg tgtcgcgcgc ggcgatggtt
     7801 tcgctccacg ccatcgccgg cgcgggcgcg agcagcagca atgcgagaaa tgtcgttttc
     7861 atatcgttcc tccaataatc cgcccgccgg gcggcgttct tcagaacctc acccgggctt
     7921 cgatgccata ggttcgcggt tcgttataca gcgttgcggt ggtcctcctc ggaatacgga
     7981 aatcgaagag attataggtc gcgttggtca gattcttgcc ccacagcgcc acttccagcg
     8041 tgccgccgcc aagatcgatg tcgcccagcg tcaaccggcc gttgagcagc agcgccttgt
     8101 cggctttgac gtcgacggtg ccgttgggaa tgtaatttcc ggcggtatcg aggccgacat
     8161 aggcgcgcag ctggccaaag gaaaaggcgg gaaagacata gtcggccgag aagctcgccg
     8221 catgtttcgg ggtgaaggcc gaggatgtct gttcgatctc gccggtaaag atgttccgca
     8281 ccggggtcgg cggcgcatcg gtgtagacat aattggccga cagcgacagg ccgcgcgccg
     8341 gaacgagcgt gatatcggcc tcgacgccct tgatcttgcg cttctcgggc gcgttgaccg
     8401 tttcggtgtt cgatatgaag gcggggttga ggaagtcgac ctgctggttg gacaggcggc
     8461 tcatataggc ggcgacgttg acgcgcagcc tgcggtcgaa caggtccgcc ttcacaccgg
     8521 cttcccacgc cgtcagctct tcttcgccaa aggtacgcaa gatgaccgag cgcgtgttgg
     8581 cgccgccggc gcgataggcc cggctccact tcacataggc gttgatgtcc tcgctgaaat
     8641 cataggcgat cgtcgccatc ggatcgacgc ggctcgaatc gaagacgaac gccagattcg
     8701 gatcgacgcc ccggatggaa agcaggcggc cgtccttgtg atcgtcggtg taacgcgcgc
     8761 cggcggtgat atgcagtccg cgcagcgcct cgggcgacca ggtcgcctgg ccgaacagcg
     8821 ccttggattt gacatcgacc agcgccgcgc gatcgggaag gttgcccgcg ccgccatcgg
     8881 gcgacggttc ggggaagaaa ttgaccccgg tcagcgcctg attaagcgtt ccgagcgaat
     8941 agacgatcgc ctcgtcctgg cccttttcct tgaaataata ggcgccgagg acgaatttca
     9001 actcgccgac atcgccgacc agctgaacct cctggctgaa ctggctctgc ttcacattgg
     9061 catagctcag gcggccgaag cggcggttcg gaccccaggt cgtgttcgat ccctcgtcgg
     9121 tgtcgagctg ggtcgagtcg agattgcgcc acgcggagat cgagcggacc gtcagcgcgt
     9181 cgctcacgtc ccattcggcg gtgatgctgt ggcccttggc ctcctgcggg ttgcggaaga
     9241 cggggaagcc gaagcgtccg gtgcgcacgc ggccggtatc gagcgtgatg aaggatggcg
     9301 tgaccgcggg cgactgatcc gacgcggtga tataatgata atagcccgtc gatttgtcgc
     9361 gcgagagatc ataggcatag agcagcgaca gattatcggc gggtcgccag agcgccgaga
     9421 tcttgaagcc gtatcgcttg atctcgccat aatcgagcgc gtcgccgccc agcggatttt
     9481 tcaccagccc gtcgcggccc tcgaacaccc cgtcgacctt gacgctgatg cccgccgcct
     9541 cgggcaggtt gacgtgcgcg gcaagggacc ggccgcgata attgccgatg cccgccttga
     9601 aatcgacgct cgcctcgccg gtcggccgct tcgacaccac gctgatcgcg ccgccgatcg
     9661 tgttgcggcc gaacaatgtg ccctgtggac cgcgcagcac ctcgatccgc tcgatgtcgg
     9721 caagctccat gccgagcccc gacacacggc cgagatagac gctgtcgata tagatgccga
     9781 cggtgggatc gcggctgacc tgcgtggcgt cgaccgggac cagcccgcgc atcccgatgc
     9841 tgaccgcgga cacgcggccg acgaagggcg cgatgcggat cgacgggacc gcgccagtga
     9901 acaggtcgga cagcgatccg atgccctttt cctcgagcgt ctccgagctg agcgcgacca
     9961 tcgagatcgg cgtgtcctgc agattctctt cgcgcttctg ggcggtgacg acgatgtcgg
    10021 tcacgccgat cgtctggtcc ccggtgcttt cctgcgctgc ggccggcgtt gcgagccagg
    10081 tggcggccga tgccgcaagc gcgatgcgta acagatgcaa tcctccgttc gacatagatt
    10141 ttccctccca ttttgcgccg gcttttgtcg cctgcgtcga ttcacctact gatgagtaga
    10201 cgaatttgta gccggagaaa ggagatttgc aagcgtcatt cgaatccgcc aaaaaggcgc
    10261 gatgcacatg accattccga tgccggcgac gcgcaaggag cgcgccgacg caacccgcga
    10321 agccattctg cacgaggccg agcgcgtgtt cgccgcgaca ggtttccggg ccgcgcggct
    10381 ggaggatatt gccgatgccg tcggtattcg gcggccctcg ctgctccatc atttctcgac
    10441 gaagaaaatg ctctacgatg ccgtcgagca gcgcatattc gaaaatatgc acgcggtctg
    10501 cgaggcgcgc actgccggtg tcgaagatcc tacggcccga ctgctcgcgc atctcgacgc
    10561 gtggctcgac ttcttcatcg accgtccgcc cgccgcccgc atccttcagc gcctcgtcgc
    10621 cgatacggcc gttcgcggcg acaatccggt ggagttttcc tatgtcgcgc tcgaagacat
    10681 ggaccggaca ttggcctatg ggatcgcgcg cggtgcgttt gttcccctat ccacgatgac
    10741 attcgtcaac ggggtggcag cgggaatcct gttcttcgtc tgcaacagcc gtcaggtcgg
    10801 cgagcagcgg cgttacgatc cttccgatcc ttcggacaag gccgtcttcc ggaccttgct
    10861 gcacaagctc gcgcgcaccg ccgtgctgcc ctgatgcatg atatgaccct aggccggagc
    10921 gacggccgcg cgggtcagca ggtggagcag ttcgcgaaag ctttcgagct ccttggggtc
    10981 ggcggcgtca taactgcggt tggagccgag ctgcttgctg ttgcagacat agaagagtga
    11041 acccgatccc accgcgttga cgacatgcat cggcggcagc ggacggaagt cgcccgcggc
    11101 gacgccttcg ttcaccacgg tctcgacatc gcgcagcagc acgttcgaga attgcgtggg
    11161 gtcgccgccg cgcggagtga cgtcggcgac gagccgctga atgatccggg cggccgacgg
    11221 ccgcgcgacg aggaaatcga gccacccgtc gaacagcgcg agcagcttgt cgatcggctt
    11281 gccctgctgg gcgatgcggc tgagcgcttc gcggtgcatg gcagcgaaga tgtcttcctc
    11341 gaccgcgtca tacagctcct gcttgttcga gaaataatag acgatcgagg ggcggcggat
    11401 gccgaccgtc gcggcgacat cctcgagcct tgccgtggtg aagccgacct cggcgaaaac
    11461 cgtttcggcc gcctcgagga ttgcctgacg cgtcgcttcg ccgcgcacat gcctgatggg
    11521 cttcatattc aatgctcaaa aaccttccgt ttccgcgctc tcacgcagcg ccccttgtgg
    11581 cgcaatcaaa tcgcgcttgc aatctacctt catgcaggta gaaatctacc tacgcgaagg
    11641 tagaatcgcg aagcgggggc gaaagcacgt ctccgatgtt catggagggg aagacgatga
    11701 agaaggttac cgggccgcag gcgcgcctgc tgctgagcac gctcgcgatc gccgcggcac
    11761 agcccgcgct ggcccaaacc gccgcaacgg cgaccgacga aagccagggc ctcggcgaaa
    11821 tcgtcgtcac cgcgcaaaag cgggccgaat cgctgcagga tacgccgatc tcgatcgcgg
    11881 ccttcaccgc cgaggagctc gaaaccaagg cgatcaccgg gctcaccgac ctgcgcgcca
    11941 acgtgcccaa ccttcagctc acgccgcacc ccaacagcgc cgcgacgacg cagatcttca
    12001 tgcgcggcgt gggcctcagc gacgaccaga tcacgcagga cggcggcgtc gcggtctata
    12061 tggacggcgt ctatattgcg cgcagccagg ggcaggcgct cgaagtcgcc gaactcgagc
    12121 gcgtcgaggt actgcgcggc ccgcagggca cgctctatgg ccgcaacgcg acgggcggcg
    12181 cgatcaactt catcaccaag aagcccgagc ttgaggattt cggtttcaag gggcaggtga
    12241 cgctcggcaa tttcaacaat cgccgcttca aggcggcagt caacgtgccc gtcggcaata
    12301 cgctggccgc ccgcttttcc tatgcgaata tccggcagga cggctttgtg aagaatccgg
    12361 gcaccggggt gaagcgctgg ggcgacaagg accgccaggc gatgcgcgcc gacgtgctgt
    12421 ggcagccctc cgacgccttc agcctgcgct acggctatga ccggtcggaa atcgaggaca
    12481 cgccgatcta tgtcgtcgat tcgccgcttc acccgctgaa ggcgccgcgt ccgaaggccg
    12541 gcagcccgct ggttcgcgac ctgttgccca acgacgtgac ctcgcagggg cacagcctga
    12601 tcgccgaatg ggaggcggcc gatgcgctca cggtccggtc gatcaccggc tatcgcaagc
    12661 tcagcaattt ccagaatcag gactatctga ccggcgcgac cggacccgat ccgctgcaga
    12721 agaacagcag cctcaccaag caggatcagt ggagccagga actgcaactg gtcggcgacc
    12781 tcctcgaccg ccagatcgaa tatgtcgcgg gcgtctatta tttctccgaa gacggcagca
    12841 atttttcgaa cagctacagc ccgccgaggc ggacgcgcag cttcacgacg gcgacgatcg
    12901 cgaacgaaag ctgggcggtc ttcggccagg cgacctacag ccccgaatgg ctcgaccgcc
    12961 ggctccacct gaccgtcggc gcgcgccaga gctgggacaa gcgcgaggcg acgctgcggc
    13021 gcgaaacgca ggtcaacgaa ggtccgatcg tgccggtgcc ggggttcggc gacggcagcc
    13081 gcaagttcac cgatttcagc cccagtttcg tgatcgccta tgacgtcgcg gacgatgtca
    13141 acgtctacgc caaggcggtg aagggctata aggcgggcgg ctataatatc cgcgcgagct
    13201 cgatcccgcg cttcaacgag ggtttcggat ccgaaacgct gtggtcgtac gaactcgggc
    13261 tgaagagcca gtggctcgac gaccgcctgc gcctgaacgt cgcggccttc cagtcgaagt
    13321 ataaggacat ccagatcaac gttcagtcgg acccgaccaa cgtccggatc accgacgtgc
    13381 tcaatgcggg caaggcgacg gtgaggggcg tcgaggtcga cctgacgctg gcgccgacgc
    13441 gcgacctgcg cttcggcatc cactacggct atctcgacgc gcaatatgac gagatcatcg
    13501 acggtgcggg caacgatatt tcggcggact atcgcttcac caacgcgccg aagaacacgc
    13561 tggcgttcga cgccaattat acgctgcccg acctgccggt gggggcgctg tcggcgaacg
    13621 tcaattatac gatgcaaagc gacaagttca ccggatcgac catcgccggc ggaaaattca
    13681 tcaccggcga ttacgggctg ctcaacgcgc ggctgacgct ggccgaaatt ccgggcgtcg
    13741 agggcgtgcg cgtcgggctg tggggccaca acctcaccga caccgaatat tatatcatgc
    13801 agttcaacat cggccgcccg ggcgcgctgt tcggcgaacc gcgcacctat ggcgtcgacc
    13861 tgacggtcga gttctgagcc gatgaacagg gccaggatcc ccgcgatcgc gctcggcgca
    13921 ctcgccctgt cgctgtccgg cgccgcattc ggcgccggac agggggcgaa ggactatcgc
    13981 cccggcgccg ccgatgtcgc gcgcacgcaa aaggattttg cgggcggtgc tagcgagttc
    14041 cgcttcgtcg tcgtcggcga ccgcaccggc aagcaccgtc cgggcgtgtt cgaggatgcg
    14101 atggacaagg tcgagcggct tcgtcccgat ttcgtcatca acatcggcga cctgatcgaa
    14161 ggtaacaccg aagatccggc gcagctcgac aaggagtgga acgaggtcaa tgtcgcgatc
    14221 gaccggctaa gcattccctt cttctatgtg ccgggcaatc acgacctgac caacgaggtc
    14281 cagcgcaccg aatggcgcaa gcggctcggc gccgattatt acagcttcac ctacaagaac
    14341 gcgctcttcc tcgtcctcaa caccgaggat ccgccgcagc ccaagatcgc gcggatgcag
    14401 ctgttccagc aatatggcgg cgaggcgatg aagacggtgt tcgaggcgct gcagggcgat
    14461 ccggccaagg ccgaggcgct gttcgcgggc gatccgaagc ttggcgagct tgccgccaag
    14521 atccgcggtt cggaaaatgt cgctttcagt gcggaccagg tgaaaatggt gcgcgacgcg
    14581 ctcgcgaaat atcccaaggc gcgctggacc ttcgtgctga tgcaccggcc cgcatggaag
    14641 gtcgattcac ccgcatttcg cgagatcgag caaatgctga aggggcgtcc gcacaccgtt
    14701 ttggcggggc attatcacaa atatgcctat gaggcgcacg gggggaccga ctatatccag
    14761 ctcgggacga cgggcggtat tcccggcggt gcggccgacg atccggcggt ggtcgatcac
    14821 gtcatgtggg tttcggtcgc cgatggcgcg ccgcgtgtca gcaacctgaa gctcgacggc
    14881 ctcttcgcaa agcaggggcc cgccgcgatc gtccgtccgg actgatccaa ccgatcatat
    14941 catggagatt gttccgcaat gagttctacc gatctttcgc ccgcgctcgg cgcgcccaac
    15001 accgctgtga tcgtcaccgg gggcgcgtcg gggatcgggc tcgcctcggc gcgcgcgctc
    15061 gccgc
//
LOCUS       DATMSU010000414         8971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_158783,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000414 DATMSU010000000
VERSION     DATMSU010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158783"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(268..1053)
                     /locus_tag="VN034_11185"
     CDS             complement(268..1053)
                     /locus_tag="VN034_11185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV61211.1"
                     /translation="MIHAPMLQGKKLVQSMFARRGYKIVRSESEIQYRPTSADARNSA
                     SLLHEIVPVVQHNALTGEWPTPEFLEHYFDASRLAMARLLLDRCDAEEVDMAGQRVLD
                     IGCHAGCLLRLMRARYPEASLFGCDISDVKLAMARRACPDAELFFCSLSDLPDASRYD
                     VVFLMEVLEHTVDPEAVVRRLLDIVSASGTLVLTVPDGRKDQFPAKEYNAEFDSYAGH
                     INFWSPESWNYFLLRIAPEWRVRTGALSTGHLFGALSMKNKAI"
     gene            1511..4021
                     /locus_tag="VN034_11190"
     CDS             1511..4021
                     /locus_tag="VN034_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61212.1"
                     /translation="MTIRGPAAASLFVLFASTSGIALAQSATNDTSAATESPGDEIVV
                     TGRAGTGDRTRIETSYAITSIDNDTLRSRAPSSVTEALKSVPGFWVEASGGEGSGNVR
                     ARGIPVDGFGSITLLENGLPVQHDPSLGYLNADQAFRIDESIERIEVVRGGPSSIFYS
                     NAPGGAVNFITRQATDELTGVARILYGPTADLFRFDGWVGAPLGNGWSFGVGGYYRKE
                     DGVRDPGFTGNQGGQVRADLDYDFGDGRITLGYKRIDDSTIFYTGIPLTKNSKGDIVG
                     LPGFNPHYGTTASRATAFLALRDANGPVQFDNADGTDVNLDQFSLLAEWEFAPGWKLS
                     NRARYRDSLTVRNGVYPASISTATAFLNQNSASLLAAFPGATSIQLRYSDTGEAFDVT
                     GQNGNGDVFINAARPVTVDESEFLNDLRLAHSFDMGGTTHDVAIGFYYARIEESFRRY
                     SASTLQDVSDNSRLLDLVALDGAGQVVGAATENGVVRYGSEFANGSGTQDSFALYATD
                     EWQILPELRVDAGIRWETVKTRGAQEGSRTVNLGNPATLADNNVLTGNGVFTPFDKKF
                     DKIGYTVGADWQFAPRAGVFARYTSAFRLPSVGSFITSATAVPRTQGIDLWEAGLKYQ
                     SRFASVYLTGFLTDFNSYGIGNTRFDPDTNGYVSQTIYTDTRAYGVEFEGTVRPAPWF
                     DFTLNATWQDPTFRNLRYDELTTVDGQQTLVPRDYTGNQLIRVPKIALRATPAVTLLD
                     DRLRAQLDVEHYTKRFADAANISKLPAYTVLNASVRFRVTDQITLWAYGDNLTNEIGL
                     TEGNPRAGELTSGQANDLLFIGRPIVGRNVRFAVDFRF"
     gene            4021..5511
                     /locus_tag="VN034_11195"
     CDS             4021..5511
                     /locus_tag="VN034_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWV61213.1"
                     /translation="MLLRATLFAAALAIAGVAAAEPPKPDVTVAGEITGADHQTYREV
                     PFDVPEGTERITVTLTYDKSNRTVVDMGVWDPARFRGWSGGTRDRFTIAPSDATPGYL
                     AGALPSGRWRVLLGVPNVRKESRAAYSVNVFFDRTGQSRASAAIADPPIKAVPGWYRG
                     DLHMHDAHSDGSCQSRGCRKVPCPLFRAVAAAAETELDFIAVTDHNTTSHFGGLRELQ
                     PYFDTLLLIPGIEVTTFGGHANIFGPTRFVDFRVGTPDVADIGTLQRDVAASGAILSL
                     NHPALPSGEQCMGCGWTWPDTDYGKVTAIEAINGTMTEGPFAGLGFWYARLNEGHRLT
                     GIAASDNHDPDVAPGNPPPGAVPIGRPRTVVHAPALSMPAILNGIRGGNVFIDIEGTR
                     TRLLEVEAVADERRAMMGEILPGRRPTTIRTHVADVAGSFVELILNGQPSGVRRPVTS
                     DDARLTLTLNAEQACGWTAVNVRNGDGKLLLVGNPIYLSCDTVAPR"
     gene            5757..7940
                     /locus_tag="VN034_11200"
     CDS             5757..7940
                     /locus_tag="VN034_11200"
                     /inference="COORDINATES: protein motif:HMM:NF020137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWV61214.1"
                     /translation="MPSLYISPTGSGDKSGSDINNAASIGSLNTLIGRAGPGGEVLLL
                     ADQGGYNVTGILGITRGGADGEPVVVRGIDSDGNAMAAQFTGSRPADWQAGDASGNEL
                     FKLGTGADNLEFRDLQIDNVGTAFRVTADLANLHIENVDANNVRRFFEDYSSAPHATA
                     TISGLTIRDVDVQGFSRVAIRLQYDTNNVVIDNVTADMAGQTGDDLPAGVHLDDTVHN
                     VTLSRVVMENIQSTAGAYFNGDGFATERGVYDVRFIETVARGNSDGGYDLKSNGTVIV
                     RALSEENGRNYRLWGEATLIDPIGLNPVLRGGISEQNQLWLDDEAQVTVIGGRFEDAG
                     SRTKVISSQGRLSLRGVEITLSELATLLTRDSLPGLSGIETIRETLTVDQGDASPGAS
                     DFITLLPLPDTATPDALQGSSGDDIFLVDDTGDRIIEAANAGFDRVETTLDRYTLGGH
                     IEQLIRRGVADFKGDGNALANAMIGGAGRDTLGGLDGNDDIAGNSGDDWLEGSNGEDL
                     LWGQSGADMLLGGWQSDWLDGGTGADRLVGDQEWLSSRGANDRLDGGDDADLLIGDAT
                     NIYGSGKGGNDRLTGGAGDDILIGDGDVMTDSARGGADTLEGGEGNDWLYGDGRESTM
                     RVSGGDDKLYGGDGPDHLIGGGGDDLLAGGAGADWFVFAPGSGADEISDFAAGADHID
                     LSAFDIAYEQLGFTASAEGVRIAIGADSIFVRGAQSFSPSDFVFG"
     gene            complement(7949..>8971)
                     /locus_tag="VN034_11205"
     CDS             complement(7949..>8971)
                     /locus_tag="VN034_11205"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWV61215.1"
                     /translation="RDRIRTAATSYTLGANVEELVYTGTIGATMTGNALDNFVVGSGG
                     EDRLAGGDGADVLRGGAGGDILDGGAGSDTVEYAGSLGGVLVNLRAQTATGGDAMGDR
                     LISIENAEGSAFADVLLGSAGANILTGGEGDDALSGDDGNDTLRGGADADRLDGGKNG
                     DALHGDEGDDMLYGGQQTDRLYGGTDNDLLVGDTEWISSLAARDSLDGGDGDDMLVGD
                     GMSMTASGVGGADTLAGGLGNDILYGDAVNMAAGARGGADKLTGGGGSDRFVFAAGSG
                     ADEISDFTRGGAEADRIDLSAFDIAYEALRFTTSSAGATIQLGGTDTIFVRGVTALDQ
                     TDFVFG"
BASE COUNT         1641 a         2986 c         2825 g         1519 t
ORIGIN      
        1 tcgatcccac cgatatcggc gccgacgaga tcaacgcacg catggcgaag ctcgccaagc
       61 gacgagcaca gggaagagcc gcctacgccg acgctaaatc ccgcgacagc taatcgatgg
      121 cggcttcctg accacgctta caggcgaaga attcgctcgc attgcgaagc ttcttgccgc
      181 gccccgcgct tgctcgatcc gatctcgcag cggtccggtc ggcgattttc gaggccgctc
      241 cggtcccggg cgccaacaga atggacgtta gatcgcttta ttcttcatgc tcagtgctcc
      301 gaacaagtgt ccggtcgata acgctcctgt acggactctc cactcgggag cgatccgcaa
      361 aagaaaatag ttccagctct ccgggctcca gaagttgata tgacccgcgt aggaatcaaa
      421 ctccgcatta tactccttgg cgggaaattg atccttccgc ccatcgggga ccgtcaatac
      481 gagggtgccg gaggcggaca cgatatcgag caggcgccga acaaccgctt cgggatcgac
      541 cgtgtgttca agcacctcca tcagaaacac cacgtcgtag cgcgatgcat caggaaggtc
      601 cgagagcgag cagaagaaca gttcagcgtc ggggcaggcc cgtctggcca tcgcgagctt
      661 cacatcactg atgtcgcatc cgaagaggga ggcctccgga tatctcgcgc gcatgaggcg
      721 caacaggcat cctgcgtgac acccgatgtc cagaacgcgc tggcccgcca tatcgacttc
      781 ttcggcatcg catcgatcaa gcagaaggcg agccatcgca agacggcttg catcgaagta
      841 gtgctcgaga aattctggtg tcggccattc gccggtcagc gcgttgtgct gcaccaccgg
      901 aactatctca tgaagcaagc tcgcggagtt gcgcgcgtcg gctgaggtcg gtcgatactg
      961 aatctcggat tcagatctta caattttgta gccgcgacgc gcaaacatgg attgaaccaa
     1021 ttttttgccc tggagcatcg gtgcatggat catctgtcct ccttcgcgcg gacgcgtcag
     1081 ttgtggaagt ccgaacccgg gtatgcctca atgctgccgc agccatccgc tatagacaac
     1141 cacgccgatt gccacacatc ccggtcgaaa cacgatcgaa cccggctccg agcagtaggg
     1201 cggccggaga gcgcgaggcc gagtatgaac gacgggactc gcaaatcctg cattgcagca
     1261 atgtcgtgag gccggtctcc cgattcgcgc agggcgttac ctcgcgacga tagccacaga
     1321 agattgctgc tcgcccaagg acacggacga agcttgggtg cattgctatc tgtcagagac
     1381 aagaaggcgg tgtcacagca atgcagcacg gctgtcacag ggacgtcctg ccgcggccat
     1441 cgaatcgcaa ccctgaagtc ctagcggacg ccgaccgcaa tcggcggcaa tccaatccag
     1501 gggtttcgga atgacgatca gaggtcctgc cgccgccagc ctatttgtcc ttttcgcgag
     1561 cacgagcggc atcgcgctgg cgcagtcggc taccaacgat acatccgccg ccaccgaatc
     1621 gccgggagac gagatcgtcg tgaccggccg cgccggcacc ggcgaccgca cgcggatcga
     1681 gacaagctat gcgatcacct cgatcgacaa cgacacgctg cgctcgcgcg cgccgtcgag
     1741 cgtcaccgaa gcgctgaagt cggtccccgg cttctgggta gaggcatcgg gcggcgaagg
     1801 cagcggcaat gtccgcgccc gcggcatccc ggtcgacggc ttcggatcga tcaccctgct
     1861 cgaaaacggc ctgcccgtgc agcatgaccc gtcgctcggc tatctgaacg ccgaccaggc
     1921 ctttcgcatc gacgaatcga tcgaacgcat cgaggtcgta cgtgggggtc cgtcgtcgat
     1981 tttctattcg aacgcgccag gcggcgcggt caacttcatc acgcgccagg caaccgacga
     2041 gctgaccggc gtcgcgcgca tcctctatgg tccgaccgcc gacctcttcc ggttcgacgg
     2101 ctgggtcggc gcgccgctcg gcaacggctg gagctttggc gtcggcggct attatcgcaa
     2161 ggaggatggc gtccgcgacc ccggtttcac cggcaatcag ggcggacagg ttcgcgccga
     2221 cctcgattat gatttcggcg atggccggat cacgctcggt tacaaaagga tcgacgacag
     2281 cacgatcttc tacaccggca ttccgctcac caaaaacagc aagggcgata tcgtcggcct
     2341 tcccggtttt aatccgcatt atgggacgac agcgagccgg gcgaccgcgt tcctggcgct
     2401 gcgcgacgcc aatggaccgg tccagttcga caatgccgac ggcaccgacg tcaatctcga
     2461 ccagttcagc ctgctcgccg aatgggaatt cgcgccgggc tggaaactca gcaaccgcgc
     2521 gcgctaccgc gactcgctca cggtgcgcaa cggggtctat ccagcgtcga ttagcaccgc
     2581 gaccgccttt ctcaaccaga atagcgcgtc gctcctcgcg gccttcccgg gcgcgacgag
     2641 catccagttg cggtacagcg acaccggcga ggcgttcgac gttaccggcc agaacggcaa
     2701 tggcgacgtc ttcatcaacg ccgcgcgacc ggtcaccgtc gatgagagcg agtttctgaa
     2761 cgacctccgc ctcgcgcaca gtttcgacat gggcggcacg acgcatgatg tcgcgatcgg
     2821 cttctactac gcccggatcg aggaaagctt ccgccgctat tcggcgtcga cgctgcagga
     2881 cgtgtcggac aattcgcggc tcctcgacct cgtcgcgctg gacggcgccg ggcaggtggt
     2941 cggcgccgcg accgaaaacg gcgtcgtccg ctacggctcc gaattcgcca acggcagcgg
     3001 cacgcaggat agttttgcgc tttacgctac ggacgagtgg caaatcctcc ccgagctgcg
     3061 cgtcgatgcc ggcatccgct gggaaaccgt caagacccgc ggcgcgcaag agggcagccg
     3121 cacggtcaac ctcggcaacc cggcgaccct cgccgacaat aatgtgctga ccggaaacgg
     3181 cgtcttcacg ccgttcgaca agaaattcga caagatcggc tacacggttg gcgccgattg
     3241 gcagttcgcg ccgcgcgccg gggtcttcgc acgctacacg tcggcttttc gtctgccgag
     3301 cgtcggcagc ttcatcacca gcgcgaccgc cgttccgcgg acgcagggta tcgacctctg
     3361 ggaagcgggg cttaaatatc agtcgcgctt cgccagcgtc tatctcacgg gtttcctcac
     3421 cgacttcaat tcctatggga tcggcaacac gcggttcgac cccgacacca acggctatgt
     3481 gtcgcaaacc atctataccg atacgcgcgc ctatggcgtc gagttcgagg ggacggtgcg
     3541 gccggcgccc tggttcgact tcacgctcaa tgcgacctgg caggatccga ccttccgtaa
     3601 cctgcgctat gatgaactca ccacggtcga cggacagcag acgctggtgc cgcgcgacta
     3661 tacgggcaat cagctgatcc gcgttccgaa gatcgcgctg cgggcgacgc ccgcggtgac
     3721 gcttctggat gaccggctgc gcgcccagct cgacgtcgaa cattatacga agcgcttcgc
     3781 cgacgccgcg aacatatcga agctgccggc ctatacggtc ctcaacgcca gcgtccggtt
     3841 ccgcgtcacc gatcagatca cgctctgggc ctatggcgac aatctcacca acgagatcgg
     3901 gctgaccgaa ggcaatccgc gcgcgggcga actcaccagc ggacaggcga acgacctgtt
     3961 gttcatcgga cgcccgatcg tcggccgcaa cgtccgcttc gcggtggact tccgcttctg
     4021 atgctgctcc gcgcaaccct gtttgccgcc gcgctggcga ttgccggcgt ggcggctgcc
     4081 gaaccgccaa agccggatgt gacggtcgcg ggcgaaatca ccggcgccga ccatcaaacc
     4141 tatcgcgaag tgccgttcga tgtgcccgaa ggcaccgaac ggatcaccgt gacgctgacc
     4201 tatgacaagt cgaaccggac ggtcgtcgac atgggcgtgt gggatcccgc gcgtttccgc
     4261 ggctggagcg gcggcacgcg cgaccgcttc acgattgcgc caagcgatgc gacgcccgga
     4321 tatctcgccg gggcgctgcc gtcggggcgc tggcgcgtgc tgctgggcgt ccccaatgtg
     4381 cggaaagaga gccgggcggc ctattcagtc aatgtcttct tcgatcgcac cgggcagtca
     4441 cgcgccagcg ccgcgatcgc cgatccgccg atcaaggccg taccgggctg gtaccgcggc
     4501 gaccttcaca tgcacgacgc gcacagtgac gggagctgcc agagtcgtgg ctgccggaag
     4561 gtgccctgcc cgctcttccg ggcagtcgcc gccgccgccg agacggaact cgatttcatc
     4621 gcggtcaccg atcataatac gacgtcgcat ttcggcggcc tgcgcgaact gcagccctat
     4681 ttcgacacac tgctgttgat ccccggtatc gaggtgacga cgttcggcgg tcatgccaat
     4741 atcttcggcc ccacgcgttt cgtcgatttt cgcgtcggaa cgccggacgt agccgacatc
     4801 ggcaccctcc agcgcgacgt cgcggcgtcc ggggcgatcc tgtcgctcaa tcatcccgcc
     4861 ttgccgtcgg gcgagcagtg catgggctgc ggctggacct ggccggatac cgattatggc
     4921 aaggtcaccg cgatcgaggc gatcaacggc acgatgaccg aggggccttt cgccgggctc
     4981 ggcttctggt atgcgcggct gaacgaaggc caccgcctta ccggcattgc ggccagcgac
     5041 aatcacgatc ccgacgtcgc gcccggcaat ccgccgcccg gtgcggtgcc gatcggccgg
     5101 ccgcgaacgg tcgttcatgc gccggcgctg tcgatgcccg cgatcctgaa cggcatccgc
     5161 ggcggcaatg tgttcatcga tatcgaggga acgcgtaccc ggcttctgga agtcgaagcc
     5221 gtggcagatg aacggcgtgc aatgatggga gaaatattgc cgggacgccg ccccacgacg
     5281 atccggacgc acgtagcgga cgtcgcaggg tcgttcgtcg aactgatcct gaacggccag
     5341 ccgagcggcg tgcgtcgacc ggtcacctcg gatgatgcta ggctcacgct gacactgaat
     5401 gccgaacagg cctgcggctg gaccgctgtc aatgttcgga acggcgacgg caagctgctg
     5461 ctcgtgggca atccgatcta cttgtcctgc gatacggttg caccgcgatg atagaatgcg
     5521 actctggccg atagcgatcg ggcagcttcg gcgagcaact ggcgacgcaa cctcaaaaag
     5581 tcatggcatt tcaatgtctc agcacgggac agaatggccc tcagcgattt tcctaccgtg
     5641 cagccatcgc aataacctct ccgatgatca ggataacact ggcttaacca cggtgccgtg
     5701 cagcatgtcg gattggtcga ggtttacctc gacgcaacag caaggggtcg ttcgtcatgc
     5761 caagcctgta tatctcgccc accggatccg gcgacaaaag tggcagcgat atcaacaacg
     5821 cagcgtcgat cggctcgctc aacacgctga tcgggcgcgc ggggccgggg ggcgaggtgc
     5881 tgctgctggc cgaccaaggc ggttacaatg tcaccggcat cctcggcatc acgcgcggcg
     5941 gggcggatgg cgagccggtt gtggtgcgcg gcatcgattc ggacggcaat gccatggcgg
     6001 cgcagttcac gggctcgcgc ccggcagact ggcaggcggg cgacgcctcc ggcaacgaat
     6061 tgttcaagct cggcacaggc gccgacaatc tcgaattccg cgacttgcag atcgacaatg
     6121 tcggcaccgc ctttcgcgtc accgccgatc tcgccaatct ccatatcgag aatgtcgacg
     6181 cgaacaatgt ccgccgcttc ttcgaggatt attcgagcgc gccgcacgct accgcgacga
     6241 tctccgggct gacgatccgc gatgtcgacg tccagggttt ctcccgggtc gcgatccgcc
     6301 tccagtacga caccaacaac gtcgtgatcg acaatgtgac ggcggacatg gccgggcaaa
     6361 cgggcgacga cctgcccgcg ggcgtccatc tcgacgacac ggtccacaac gtcacgctca
     6421 gccgggtggt gatggagaat atccagagca cggccggcgc ctatttcaac ggcgatggct
     6481 tcgcgaccga gcgcggcgtc tatgatgtgc gcttcatcga aacggtcgcg cgcggcaaca
     6541 gcgacggtgg ctacgacctc aagtcgaatg gcaccgtgat cgtccgcgcg ctcagcgagg
     6601 agaacggccg caactaccgg ctctggggcg aggcgacgct gatcgacccg atcggcctca
     6661 acccggtgct gcgcggcggc atctccgagc agaaccagct ctggctcgac gacgaggccc
     6721 aagtcaccgt gatcggcggg cgcttcgagg atgccgggtc gcgcaccaag gtgatctcct
     6781 cgcaggggcg gcttagcttg cggggcgtcg agatcaccct gtccgaactc gcgactttac
     6841 tcacccgcga ttcgcttccc ggcctcagcg gcatcgagac gatccgcgaa acgctgacgg
     6901 tcgatcaagg cgacgcctca cccggtgcga gcgactttat cacgctgctt cccctgcccg
     6961 acaccgccac gccggatgcg ctacaaggca gctcgggcga cgacattttc cttgtcgacg
     7021 acaccggtga ccggatcatc gaggccgcga acgccggctt cgatcgggtc gaaacgacgc
     7081 tcgaccgcta cacgctcggt ggtcatatcg agcagctgat ccgccgcggc gtcgccgatt
     7141 tcaagggcga tggcaacgcg ctcgccaatg cgatgatcgg cggtgcgggc cgcgacacgc
     7201 tgggcgggct cgacggcaat gacgacatcg ccggcaattc gggagacgat tggctcgagg
     7261 gcagtaacgg cgaggacctg ctctggggcc agtcgggcgc cgacatgctg ctcggcggct
     7321 ggcagtcgga ttggctcgac ggcggcacgg gcgcggaccg cctggtcggc gaccaggaat
     7381 ggctgagcag ccggggcgcc aacgaccggc tcgacggcgg cgacgatgcc gatctgctga
     7441 tcggcgatgc gaccaacatc tatggcagcg gcaagggggg caatgaccgg ctgaccggcg
     7501 gcgcggggga tgacatcctg atcggcgacg gcgacgtgat gaccgacagc gcccggggcg
     7561 gggccgacac gctggagggc ggcgagggca acgactggct ctatggcgac ggccgcgagt
     7621 cgaccatgag agtgtccggc ggcgacgaca agctctatgg aggtgatggc cccgatcacc
     7681 tgatcggcgg cggcggcgac gatctgctgg cgggcggcgc gggggccgac tggttcgtgt
     7741 tcgcgcccgg ctcgggcgcc gacgagatca gcgatttcgc cgcgggggcg gaccatatcg
     7801 acctcagcgc cttcgacatc gcctatgagc aactcggttt cacggcgagc gcggagggag
     7861 tccgcatcgc catcggcgcc gacagcatat tcgtccgcgg cgcgcagagc ttttcgccct
     7921 ccgacttcgt cttcggctga cgcgcggctc agccgaagac gaagtccgtc tgatcgagcg
     7981 cggtcacccc gcgcacgaag atcgtatcgg tgccgcccag ttggatcgtc gccccggcgc
     8041 tgctcgtggt gaagcgcagc gcctcatagg cgatgtcgaa ggcgctgagg tcgatccggt
     8101 cggcctcggc gccgccgcgg gtgaaatcgc tgatctcgtc ggcgcccgag ccggccgcga
     8161 agacgaagcg gtcgctcccg cccccgccgg tcagcttgtc cgccccgccc cgtgcaccag
     8221 ccgccatatt caccgcgtca ccgtataata tgtcgttgcc gagcccgccg gccagcgtgt
     8281 ccgcgccccc gaccccgctc gccgtcatgc tcattccgtc gccgaccaac atgtcatcgc
     8341 catcgccgcc gtcgaggctg tccctcgccg cgagcgacga gatccactcg gtatccccga
     8401 ccagaaggtc gttgtccgtg ccaccatata gtcggtcggt ctgctggccg ccgtaaagca
     8461 tgtcgtcgcc ctcgtcgccg tggagcgcat ccccgttctt gccgccatcg agccggtccg
     8521 cgtcggcgcc gccgcgcagc gtgtcgttgc cgtcgtcgcc ggagagcgca tcatcgcctt
     8581 caccgccggt cagtatattg gcgccggcgc tgccgagcag cacatcggca aaggccgagc
     8641 cttcggcatt ttcgatactg atcagcctgt cgcccatggc gtcgccgccg gtcgcggtct
     8701 gggcgcgcaa attgaccagc acgccgccga gcgagccggc atattcgacc gtgtccgatc
     8761 ccgcgccgcc gtcgaggata tcgccacccg ccccgccgcg cagtacgtcc gcgccgtcgc
     8821 cgccggccag ccggtcctcg ccgccactgc cgacgacgaa attgtcgagc gcattgcccg
     8881 tcatcgtcgc gccgatcgtc ccggtataga cgagctcctc gacattggcg ccgagcgtgt
     8941 agctggtcgc ggcggtgcgg atgcggtcgc g
//
LOCUS       DATMSU010000415         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_130594,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000415 DATMSU010000000
VERSION     DATMSU010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130594"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..374)
                     /locus_tag="VN034_11210"
     CDS             complement(30..374)
                     /locus_tag="VN034_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWV61216.1"
                     /translation="MTITRIQAGARMSQAVVHGDTVYLAGQVGEPGESVTNQTQAILG
                     QIEALLAEAGSEKAKILSATIWLADMADFAEMNAVWDKWVDGKDAPARATGEAKLATP
                     DYKVEIIVIAAR"
     gene            complement(408..1667)
                     /locus_tag="VN034_11215"
     CDS             complement(408..1667)
                     /locus_tag="VN034_11215"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HWV61217.1"
                     /translation="MKVVVLGSGVIGVTSAWYLAEAGHEVVVVDRQPGPALETSFANA
                     GEISPGYSSPWAAPGIPQKAVRWLMMRHAPLILRPAVDREMLGWLVAMLRNCTNARYA
                     INKERMVRLAEYSRDRLIALRADTGIAYDERMQGTLQLFRTQKQLDGTAKDIAVLRAG
                     GVPFELLDRQGCIAAEPGLAAAREKIVGGLRLPGDETGDCFKFTGALAAMAAERGVQF
                     RYGETIRSLAVADGRIAGVETDRGRIDADRYVLALGSHSAGLLRPLGLNLPVYPVKGY
                     SITAPILDASRAPESTLMDESYKIAITRLGDRIRVGGMAEISGYNNALPARRRATLVH
                     SLTDLFPGAGDTEGASYWSGLRPMTPDGTPVIGTTSYENLFLNTGHGTLGWTMACGSG
                     QLIADLVSGRPTDIEAADLSIARYDPR"
     gene            complement(1664..2779)
                     /gene="alr"
                     /locus_tag="VN034_11220"
     CDS             complement(1664..2779)
                     /gene="alr"
                     /locus_tag="VN034_11220"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWV61218.1"
                     /translation="MSGGRLRIDLDTIAANYRIMSERVAPAEVGAVVKANAYGLGASQ
                     VARALCNMGCRLLFVAHLGEALALRGGLPAGTRFIILNGLPAGAEASCAEAGIIPVLN
                     SPEQARRWAEAAHGIGTRLPAALQLDSGMGRLGIAPEDLAPLAADARFRSAVDLVMVM
                     SHLACADTPEHPANAAQLAAFEAAAALFPDIPRALSNSGGAFLQPEYHADIVRAGIAL
                     YGGSPNRSAPNPMRPVVGLEARIIQIRTIAAGGGVGYGHSHRCARESRIATVGVGYAD
                     GLPRSLGNRGAAWHGGVRLPIVGRVSMDSITIDVTAVPQERIAAGDWVELIGPHQSID
                     RLADDAGTISYEMLTSLGARFDRTYVEETMTNWGEAA"
     gene            2936..3397
                     /locus_tag="VN034_11225"
     CDS             2936..3397
                     /locus_tag="VN034_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWV61219.1"
                     /translation="MDSIDRNIVRLLRLNARIPNSQLAAEVGLSASACLRRVSMLEAR
                     GVIRGYTAIVAAPEEGGVIAIVRITLERQTEAYLNRFEEAVRQHPEIEECFLMTGDAD
                     YLLRASAASAAAYEGIHKNILSRLPGVERIHSSLAMRSVLSHIPARGRRGA"
     gene            complement(3547..3948)
                     /locus_tag="VN034_11230"
     CDS             complement(3547..3948)
                     /locus_tag="VN034_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61220.1"
                     /translation="MDAVLQRISRRISADGAHALPDADKLIWNTAIVIALMTGDTRIA
                     LPPGAEIGSWGAARAGLREMALPQAAELVRLLVLELAYRADLGGRDREAGMASLLRIA
                     DLKQRFQEIAAEVDITVKFRTMMEHLLERRS"
     gene            complement(4023..5255)
                     /locus_tag="VN034_11235"
     CDS             complement(4023..5255)
                     /locus_tag="VN034_11235"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HWV61221.1"
                     /translation="MAKHSEMIASKACATSIDFETALAILASSASPLDEVKVPLADAG
                     DCYLRSPVHARIDAPRFDCAAMDGYAIRDRDLIGGGGTFDQIGVSYAGARDAGEIGSG
                     QTVRVMTGAPLPRGADRVVMIEHCQPSNGAVRIDAPPVGKPHIRRRGSDFTRDQLLIP
                     AGAKVTPATVIAAAAADHSHLNVSRRPNVRLIATGDEIAAPGTVTGTRVTIPDSLTAA
                     IELLCHSAGARLRASTQRPDDPDAIASAGDPASTDIVVVLGGASRGDRDHSRAAFASL
                     GLEIAFADVAIKPGKPVWYGKAGTTHILGLPGNPAAALTVARIFLVPLIVQLMGGDVS
                     DALPWRLLPAVRAIEANGPREAFLCAATSYHGVEIGERQEASGQARLAGTDALVRRPA
                     DAAPVPPGTLVPTLALCC"
     gene            complement(5242..>5589)
                     /locus_tag="VN034_11240"
     CDS             complement(5242..>5589)
                     /locus_tag="VN034_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWV61222.1"
                     /translation="VTAHSRRGAALDIDWASLTGGGSTLAFYMGRDAAGDIMRGLMRA
                     GMTGNTPVMIACHVSAPDERRLATRLDLLELATKSFAADAPTLILVGAAVAPAEAVPA
                     RFEKIAGPQHGQA"
BASE COUNT          985 a         1789 c         1815 g         1000 t
ORIGIN      
        1 ggcagaagcc ggtctctgcc cagttcggat cagcgtgctg cgatgacgat gatttcgacc
       61 ttatagtcgg gggtcgcgag cttggcttcg ccggtcgcgc gggcaggggc atccttgccg
      121 tccacccatt tgtcccaaac ggcgttcatt tcggcgaagt ctgccatgtc tgcgagccag
      181 atcgtcgccg acaggatctt cgccttttcc gagcccgctt cggcgagcaa cgcctcgatc
      241 tggccgagga tggcctgcgt ctggttcgtg acgctttcgc caggttcgcc aacctggcct
      301 gcaagataga ccgtgtcgcc gtggacgacg gcctggctca tgcgtgcgcc ggcctggatg
      361 cgggtgatcg tcatgtcttt tcctttcatg ttgtggagga tcgggagcta gcgcgggtca
      421 tagcgcgcga tcgacagatc ggcagcctcg atgtcggtcg ggcgcccgct gacgagatcg
      481 gcgatcagct gtcccgagcc gcacgccatc gtccagccga gagtgccatg cccggtattg
      541 agaaacagat tttcatacga tgtcgtgccg ataaccggcg tcccgtcggg cgtcatgggc
      601 ctgagccccg accagtagct cgcgccttcg gtatcgcccg cacccgggaa caggtccgtc
      661 agggaatgga cgagcgtggc gcggcggcgc gcggggagag cgttgttata gcccgaaatc
      721 tcggccattc cgccgacacg aatgcgatct ccgaggcgcg tgattgcgat cttgtagctt
      781 tcgtccatca acgtcgattc gggagcgcgt gacgcgtcga ggatcggggc ggtgatcgaa
      841 tagcctttca caggatagac cggcaggttg aggccgagcg gccgcagcaa ccccgccgaa
      901 tggctgccga gcgccaggac gtagcgatcg gcatcgatcc ggccgcgatc ggtttcgact
      961 cccgcgatgc gcccatccgc gaccgccagc gaccggatcg tctccccgta gcggaactgc
     1021 acgccgcgct ctgcggccat cgccgcgagc gcgcccgtga atttgaagca gtcgcccgtt
     1081 tcatcgccgg gcaggcgaag accgccgaca atcttctcgc gcgccgcggc aaggccgggt
     1141 tcggccgcga tgcagccttg gcgatcgagc agttcgaacg gcactccgcc ggcgcgaaga
     1201 acggcgatgt ccttggcggt tccgtcgagc tgcttctgcg tcctgaacaa ctgcagcgtt
     1261 ccctgcatgc gttcgtcata ggcgatgccg gtgtcggctc gcagcgcgat gagccggtcg
     1321 cggctatatt ccgcaagccg caccatccgc tccttgttga tcgcataacg ggcatttgtg
     1381 caattgcgca gcatcgcgac cagccaaccg agcatctcgc gatcgacggc gggtcgcagg
     1441 atcagcggtg cgtgccgcat catcagccag cggaccgcct tttgcggaat gcccggcgcc
     1501 gcccatggcg aggaatagcc gggcgaaatt tctccggcat tggcaaagct cgtttcgagt
     1561 gccgggccgg gctggcgatc gacgacgacc acttcatggc ccgcctcggc cagataccag
     1621 gccgatgtga cgccgattac gccgctgccg agaacgacga ctttcatgct gcctctcccc
     1681 aattggtcat cgtctcttcg acgtaggtgc gatcaaatcg cgcgccgagg ctggtcagca
     1741 tctcgtagga aatggtgccg gcatcgtcgg ccagccggtc gatcgattga tgcgggccga
     1801 tcaattcgac ccagtctccg gccgcgatgc gttcttgcgg cacggcggtg acgtcgatcg
     1861 tgatgctgtc catcgacacg cggccgacga tcggaagcct tactccgccg tgccatgccg
     1921 cgccgcggtt tcccagcgat cggggcaatc cgtcggcata gccgaccccg accgttgcga
     1981 tgcggctttc gcgtgcgcaa cgatgactat ggccataacc gacgccgccc ccggccgcga
     2041 tcgtccggat ctggatgatg cgggcttcga gtccgacgac cggccgcatc ggattggggg
     2101 cgctgcggtt cggactgccg ccgtaaagcg cgatgccggc ccggacgata tcggcatgat
     2161 attcaggctg gaggaaggct ccgccggaat tcgagagcgc acggggaatg tccggaaaca
     2221 gcgcggcagc ggcctcgaag gcggcgagct gtgcggcgtt ggcgggatgc tcgggtgtgt
     2281 cggcgcacgc aaggtggctc atgaccatga cgagatcgac cgccgatcgg aaacgggcgt
     2341 cggcggcaag cggcgccaga tcctccggcg ctattccaag ccggcccatt ccgctgtcga
     2401 gctgaagcgc ggcggggagg cgcgtgccga taccgtgcgc ggcctccgcc caacgacggg
     2461 cctgctcggg cgaattgaga accgggatga tgccggcttc ggcgcacgag gcctcggcgc
     2521 cagctggcag gccgttgagt atgataaatc gggttccggc tggcagaccg ccgcggagcg
     2581 ccagtgcctc gcccagatgt gcgacgaaga ggaggcggca ccccatgttg cacagcgccc
     2641 gtgcgacctg ggacgcaccg aggccatatg catttgcctt cacaacagcg cccacctcgg
     2701 caggcgcgac acgctcggac attatccggt aattggccgc gattgtatcg agatcgatac
     2761 gcagtcgccc gccgctcatg cccccaagat ccatgtcatc gatccccgcc gatccattcc
     2821 atcgcgccgc ttctaggaaa atatcattcg aatgtttgtt caaaatgtta ccaaaatcgc
     2881 cataatatga tattctacgc aatgatcagc gaagaagatg gaatgatatt gcgtcatgga
     2941 ttctattgac cggaacatcg tgaggttgtt gcgtctcaac gcccgcatcc cgaacagcca
     3001 gctggcagcg gaagtgggct tatcggcatc ggcctgtttg cgtcgcgtca gcatgctcga
     3061 ggcgcgcgga gtgatccgcg gttacaccgc tatcgttgcc gcgccagagg aaggcggcgt
     3121 catcgcgatc gtccgcatta cgcttgagcg gcagacagag gcctatctta accggtttga
     3181 ggaagcggtg cgtcagcatc ccgagatcga ggaatgtttc ctgatgacag gcgatgccga
     3241 ttatctgctc cgggcctcgg cggcgagcgc ggctgcctat gaggggatcc acaagaatat
     3301 cctttcgcgc ttaccgggtg tcgagcgcat ccattctagc ctcgcaatgc gcagcgtgct
     3361 tagccatata cctgcacggg gcaggcgtgg cgcgtagccg ggcctgggct ttcgccaaga
     3421 tggacaagga tagtgaatcc tcagtccccg tccgaactcg cgactgccac actagaccca
     3481 ccgcgaacgt ccgcttcttt cgatctctcg cctgaaaccg gtctgtccgc aaacggccaa
     3541 agtgtatcat gatcgccgct ccaatagatg ctccatcata gtgcgaaact tgacggtgat
     3601 atccacttcg gctgcgattt cctggaacct ctgcttcagg tcggcaatgc ggagcaggga
     3661 tgccattccg gcttctcgat ctcgcccacc cagatccgcg cggtaggcca gttccagaac
     3721 aagcaatctg accagctctg ctgcttgcgg tagcgccatc tcgcgcaacc cggctcgcgc
     3781 agccccccaa ctgcctatct cggcaccggg tggtagggca attcgggtgt ctccggtcat
     3841 gagggcaatc acgatcgccg tgttccagat caacttgtcg gcatctggaa gcgcgtgggc
     3901 gccatcggcc gaaatcctcc gcgagatgcg ctgcagaaca gcatccacag cagaatgcct
     3961 gacggcagtg ggcgaagaga cattctggga agctggcggc gcgagacggc gggcaagcaa
     4021 gcctaacagc atagggcaag cgtcggcaca agcgtgccag gagggaccgg cgcggcatct
     4081 gccgggcgcc ggactagcgc atcggtacca gccagccgcg cctgccccga ggcctcctgc
     4141 cgctcgccaa tctccacacc atgatacgat gtcgccgcgc acagaaacgc ctcgcgcggg
     4201 ccattcgcct cgatcgcgcg aacggccgga aggagccgcc agggcagggc gtcggagacg
     4261 tcgcccccca tcaactgcac gataagcgga acaagaaata tccgcgcgac cgtgagagcg
     4321 gccgccgggt tgccgggcag tcccagaata tgggtcgtcc cggcctttcc ataccacacc
     4381 ggcttgcctg gtttgatggc gacgtcagcg aatgcgatct caaggccgag ggaggcaaag
     4441 gccgcgcggc tatggtcgcg atcgccgcgt gaagcgccgc cgagaaccac gacgatgtct
     4501 gtcgatgcag ggtcgcctgc gctcgctatc gcgtccgggt cgtccggccg ttgggtgctc
     4561 gctcgcagcc tcgcgcccgc agagtgacag agaagctcga tcgcggcggt cagactgtcg
     4621 gggatggtga cgcgggtgcc ggtaaccgtg ccgggcgcag ctatctcgtc gcctgtggct
     4681 ataagtcgga cgttcgggcg ccgcgataca tttaggtggc tatggtcagc cgcggcagcg
     4741 gcaatgacgg tcgccggcgt tactttcgct ccggccggga tcagcaattg gtcgcgcgtg
     4801 aaatccgaac cgcggcgtcg gatgtgcggc ttgcccaccg gcggggcgtc gatccgcacg
     4861 gcgccattgc tcggctggca atgctcgatc ataacgaccc ggtcggcgcc gcggggcagc
     4921 ggcgcgccgg tcatcacgcg caccgtctgg ccggacccga tctcgcctgc gtcacgggcg
     4981 cccgcgtagc tcacgccgat ctgatcgaat gttccgccgc cgccaataag gtcgcggtcg
     5041 cggatcgcgt aaccatccat cgcggcgcag tcgaaccgag gtgcgtcgat ccgcgcatgg
     5101 acgggcgagc gaagatagca atcgccggca tcggcgagcg ggacctttac ctcgtcgagg
     5161 ggtgatgcag aggaggcgag aatggccagc gccgtctcga aatcaatcga cgtggcgcat
     5221 gccttcgacg cgatcatctc gctatgcttg gccatgttgt ggccccgcga tcttttcgaa
     5281 cctggccggg actgcttcgg cgggggcgac tgccgcacct actaggatga gcgtcggcgc
     5341 gtcagccgca aagcttttcg tggcgagttc gagcagatcc agccgcgtcg caagacgccg
     5401 ttcgtcgggg gcgctgacgt ggcaggcgat catcaccggc gtgttgcccg tcatgcccgc
     5461 gcgcatcagg ccgcgcatga tatctcccgc cgcatcgcgc cccatgtaga aggcaagggt
     5521 cgacccgccg ccggtgagcg aggcccagtc gatgtcgagc gccgcgccgc ggcggctgtg
     5581 cgcggtgac
//
LOCUS       DATMSU010000416         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_64683, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000416 DATMSU010000000
VERSION     DATMSU010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64683"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..>2655)
                     /gene="gltB"
                     /locus_tag="VN034_11245"
     CDS             complement(50..>2655)
                     /gene="gltB"
                     /locus_tag="VN034_11245"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWV61223.1"
                     /translation="VGMAMVLAAAAVHTHLVRKGLRSYASINVRSAEVLDTHAFAVLV
                     GVGATTVHAYLAEAAIADRQARGLFGELSLDDCRLRFRKAIDDGLLKILAKMGIAVIS
                     SYRGGYNFEAVGLSRALVNDLFPGMPAKISGEGYQSLFINATEKHEAAFDGRVTTLPI
                     GGFYRQRAGGEAHAYSAQLMHLLQTAVATDSYSTYLQFARGVADLPPIYLRDLMEFNY
                     PAQGVALDSVEAITEIRKRFVTPGMSLGALSPEAHETLAIAMNRIGAKAVSGEGGEAS
                     ERYRPYANGDNANSNIKQIASGRFGVTAEYLGACDEIEIKVAQGAKPGEGGQLPGFKV
                     TEFIARLRHATPGVTLISPPPHHDIYSIEDLAQLIYDLKQINPKARVCVKLVSSAGIG
                     TVAAGVAKAHADVILVAGNTGGTGASPQTSVKYAGTPWEMGLSEVNQVLTLNGLRHRI
                     RLRTDGGLKTGRDIVIAAILGAEEFGIGTLSLVAMGCIMVRQCHSNTCPVGVCTQDEK
                     LRQKFTGSPEKVINLMTFIAEEVREILAKLGVTSLDEVIGRTELLRQVSRGAEHLDDL
                     DLNPILAKVDAPDDQRRSQGPTFRNPVPDSLDAQILSDAKPLFERGERMQLTYNVRNT
                     HRAVGTRLSAEVTARFGMKGLADDHVQVRLRGTAGQSLGAFLCQGVTLEVFGDANDYV
                     GKGLSGGRIIVRPTVSSPLVSQHNSIVGNTVLYGATAGTLLAAGQAGERFAVRNSGAR
                     VVVEGCGANGCEYMTGGTAVILGPVGSNFGAGMTGGMAFILDTDEQFERRVNGESIVW
                     QRLASGHWEAVLKELVEEHAKATGSKWSAEVLADWDRWRTRFWQVCPKEMLSRLTHSL
                     NEEEVEVVAAE"
BASE COUNT          413 a          909 c          887 g          446 t
ORIGIN      
        1 gctgaacggc ggcggcggtg cgacgaagcg cgccgccgcc gcgacgggtt cactccgccg
       61 cgacgacctc gacctcttcc tcgttcagcg aatgcgtcag gcggctcagc atttccttcg
      121 ggcacacctg ccagaaacgc gtccgccagc ggtcccagtc ggcgagcacc tcggccgacc
      181 atttgctgcc ggtggccttg gcatgctcct cgaccagctc cttgagcacc gcttcccaat
      241 ggccgctcgc gagccgctgc cagacgatcg attcgccgtt gacgcggcgc tcgaactgct
      301 cgtcggtgtc gaggatgaag gccatgccgc cggtcatgcc cgcgccgaag ttcgagccca
      361 ccgggccgag gatcaccgcg gtgccgccgg tcatatattc gcagccgttc gcgccgcagc
      421 cctcgacgac gacgcgcgcg cccgagttgc ggaccgcgaa ccgctcgccc gcctgacccg
      481 ccgcgagcaa ggtgcccgcg gtcgcgccat agagcacggt gttgccgacg atgctgttgt
      541 gctggctgac cagcgggctc gacacggtcg ggcgcacgat gatccgcccg cccgacagcc
      601 ctttgccgac atagtcgttc gcatcgccga acacttcgag cgtcacgccc tggcacagga
      661 aggcgcccaa ggactggccc gcggtgccgc gcaggcgcac ctggacatgg tcgtcggcga
      721 gccccttcat cccgaagcgc gcggtgacct cggccgacag gcgcgtgccg acggcgcggt
      781 gcgtgttgcg gacattatag gtcagctgca tgcgctcgcc gcgctcgaac agcggcttgg
      841 cgtcgctcaa gatctgcgcg tcgaggctgt cgggcaccgg gttgcggaat gtcggcccct
      901 gcgagcggcg ctggtcgtcg ggcgcgtcga ccttggcgag gatcggattg aggtcgaggt
      961 cgtcgagatg ctcggcgccg cggctgacct gacgcaaaag ctcggtgcgg ccgatcacct
     1021 cgtcgaggct cgtgacgccg agcttcgcca atatctcgcg cacttcctcg gcgatgaagg
     1081 tcatcaggtt gatcaccttc tcggggctgc ccgtgaactt ctggcgcaat ttctcgtcct
     1141 gcgtgcagac gccgacgggg caggtgttcg aatggcactg gcgcaccata atgcacccca
     1201 tcgcgacgag gctcaacgtc ccgatgccga actcctcggc gcccaaaatc gccgcgatga
     1261 cgatgtcgcg cccggtcttg agcccgccgt cggtgcgcag gcggatgcgg tgacgcaggc
     1321 cgttcaaggt cagcacctga ttgacctcgc tcagccccat ttcccacggc gtgccggcat
     1381 atttgacgct cgtctgcggc gacgcgccgg taccgccggt gttgcccgcg acgaggatga
     1441 cgtcggcgtg cgctttcgcg acacccgccg cgacggtgcc gatgcccgcc gagctgacga
     1501 gcttcacgca gacgcgcgcc ttcgggttga tctgcttcag gtcgtagatg agctgcgcga
     1561 gatcctcgat cgaatagatg tcgtggtgcg gcggcggcga aatcagcgtc acccccggcg
     1621 tcgcgtggcg cagccgcgcg atgaactcgg tgaccttgaa tccgggaagc tggccgccct
     1681 cgccgggctt ggcgccctgc gcgaccttga tctcgatctc gtcgcacgcg ccgagatatt
     1741 cggcggtgac gccgaagcgg ccgctcgcga tctgcttgat attgctgttg gcgttgtcgc
     1801 cattggcata ggggcggtaa cgttcgctcg cttcgccgcc ttcgcccgac accgccttgg
     1861 cgccaatgcg gttcatcgcg atcgccagcg tctcgtgcgc ttcgggcgac agcgcgccca
     1921 aggacatgcc gggcgtgacg aagcgcttgc ggatctcggt gatcgcctcg acgctgtcga
     1981 gtgcgacacc ctgcgccgga tagttgaact ccatcagatc gcgcaggtaa atcggcggca
     2041 ggtcggcgac cccgcgcgcg aattgcagat aggtcgaata gctgtcggtc gcgaccgcgg
     2101 tctggagcag atgcatcaac tgcgccgaat aggcgtgcgc ctcgcccccc gcacgctggc
     2161 gatagaagcc gccgatgggg agggtggtga cgcgcccgtc gaacgccgcc tcatgcttct
     2221 cggtcgcgtt gatgaacagc gattgatagc cctcgcccga aatcttcgcc ggcatgccgg
     2281 ggaagaggtc gttgacgagc gcgcggctga gccccaccgc ctcgaaatta taaccgccgc
     2341 gataggagga gatcaccgcg atccccatct tcgcgaggat cttcaaaagc ccgtcgtcga
     2401 tcgccttgcg gaagcgcagg cggcaatcgt cgaggctcag ctcgccgaac agcccgcgcg
     2461 cctgccggtc ggcgatcgcc gcttcggcca gataggcgtg gacggtcgtc gcgccgacgc
     2521 cgacgagcac ggcaaaggcg tgggtgtcga gcacctcggc cgaacgcacg ttgatcgagg
     2581 catagctgcg cagccccttg cgcacgaggt gggtgtggac ggcggcggcg gcgagcacca
     2641 tcgccatgcc gacgc
//
LOCUS       DATMSU010000417         3570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_111238,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000417 DATMSU010000000
VERSION     DATMSU010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111238"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1174
                     /locus_tag="VN034_11250"
     CDS             35..1174
                     /locus_tag="VN034_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61224.1"
                     /translation="MDFTYTETQDMIRDTLARFLGDTYDFETRQKFINSDSGRDPAIW
                     TALAQELGMLGAPFAEEHGGLGGGALENAIVMEELGKVIGIEPYLPTVVIAGGALKAV
                     GGAQADAMIPEIIAGNAIVAFAYAEPQGRYDLANLRTTAKKDGAGYVLNGHKGVVYAA
                     PWATHLLVTARTGGGQRDRDGVSLFLIDANLPGIVRRDYPTVDGSRASEIYFENVAIP
                     GDALLGPEGAGLPLVEQIVDEATVAVCAEATGVMQKLHEGTLEYTQQRKQFGVPIAKF
                     QVLQHRMVDMFMEVEQARSMTIMATLKLGLPANERMAAVSAAKNKVARGANFVGQNAI
                     QTHGGIGITQELAIGHYFKRATMIEGQFGSADFHMDRYERITLAD"
     gene            complement(1408..>3570)
                     /locus_tag="VN034_11255"
     CDS             complement(1408..>3570)
                     /locus_tag="VN034_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61225.1"
                     /translation="VPRPVPQPRAREPANDMIAAARDEIIVTASKTDLPHSHFAGVVT
                     QLDGDDLAFGGERGMDSILSRTATVSSTHLGSGRNKLFIRGIADSSFTGPTQSTVGQY
                     FGDIRLSYNAPDPDLRLYDIDNVEILEGPQGTLYGAGSLGGIIHVVPKAPDPRAMSVQ
                     AIAGLSVTQHGDPGGDIAAIANLPVGEDGHALRLVGYMLTDGGYIDNPLRGQNDVNRV
                     HVRGGRGTFRLEAGDGWTVDFGGIYQAITSDDAQYADKDAPPLTRASMVEQNATARYG
                     MGTIVVMKDWGDLYFQSSNAFIDHRLFERFDASLPESRRGDGDDAGVAMGAVDVERLF
                     APVEITPMADVPRVLDQHNDTRMFVSENRLSRPYHDGLGWVVGASFINNRARQDREYA
                     YGPLRAVLPGVTNRITEVTGYAEATVEIMPNLIASGGLRLSHARLGGEAEGVSFALAE
                     ANRATTASRNETDLLPSVSLLATPLDNVRLYARYQEGFRPGGLAVDGNFVRRFLNDKV
                     RTWEAGMRFGDKGRSLLDASISISHSRWRNIQADFIDSTGFPTTANIGNGRITSLSGA
                     VAMRPTDALTFELGAVYNHSRVDDLSPQILPVFAAAPARLGRIPNVASHAVRGSVNYA
                     TMIGDEDFRVNGWANYIGPSRLGIGPVLGESQGDYVDTGLAMRVGNNRRGLSLTLTNL
                     FDSRGNRFSLGTPFLEGNEGFLTPLRPRTLRIAVDVAY"
BASE COUNT          634 a         1139 c         1208 g          589 t
ORIGIN      
        1 gcccccgcct tcgcgggggc gacggggata aaaaatggat ttcacctaca ccgaaacgca
       61 ggacatgatc cgcgacacgc tcgcccgctt cctcggcgac acctatgatt tcgagacgcg
      121 ccagaagttc atcaactcgg acagcggtcg cgatcccgcg atctggaccg cgctcgcgca
      181 ggaactcggt atgctcggcg cgccctttgc cgaggagcat ggcggcctcg gcggtggcgc
      241 gctcgaaaac gcgatcgtaa tggaggaact cggcaaggtc atcgggatcg agccctatct
      301 gccgaccgtc gtcatcgcgg gcggcgcctt gaaggccgtc ggcggcgcgc aggccgacgc
      361 gatgatcccc gagatcatcg cgggcaatgc gatcgtcgct ttcgcctatg ccgagccgca
      421 gggccgctac gacctcgcca atctgcgcac cacggcgaag aaggatggcg cgggctatgt
      481 gctaaacggc cacaagggcg tcgtttacgc cgcgccttgg gcgacgcacc tgctcgtcac
      541 cgcgcgcacc ggcggcggtc agcgggacag ggacggcgtg tcgttgttcc tgatcgacgc
      601 caacctgccg ggcatcgtcc gccgcgacta tccgaccgtc gacggcagcc gcgcatcgga
      661 aatctatttc gaaaatgtcg cgatccccgg cgacgcgctg cttgggcccg agggcgcggg
      721 cctgccgctg gtcgagcaga tcgtcgacga agcgacggtg gccgtctgcg ccgaagcgac
      781 cggcgtgatg cagaaactgc acgaaggcac gctcgaatat acgcagcagc gcaagcagtt
      841 cggggtgccg atcgcgaagt tccaggtgct gcagcaccga atggtcgaca tgttcatgga
      901 agtcgaacag gcgcgctcga tgacgatcat ggcgacgctg aaactgggcc tgcccgcgaa
      961 cgaacgcatg gccgcggtgt cggcggcgaa gaacaaggtc gcgcgcggcg cgaatttcgt
     1021 cgggcagaat gcgatccaga cccacggcgg catcggcatc acgcaggaac tggcgatcgg
     1081 ccattatttc aagcgcgcga cgatgatcga gggccagttc ggcagcgccg acttccatat
     1141 ggaccgttac gaacggatca cgctggcgga ttgatcggta gcccctccct tggcggggag
     1201 gggcatcgac gatggcgacg ggcttgatga tggtggcttg cgatcggaag ctgccatttc
     1261 tctcatcgtc accctgaact tgtttcaggg tccatggccc ggcatttcgg atagcgccgc
     1321 acgggatgag aggtcaggcc atggatgctg aaacaagttc agcatgacga tagtggaggc
     1381 agttctccac gccaaaagcg tcattcgcta ataagccacg tccaccgcga tccgcagcgt
     1441 gcgcgggcgc agcggggtga gaaagccctc gttcccctca aggaacgggg ttcccagcga
     1501 aaagcggttg ccgcgcgaat cgaacagatt ggtgagcgtc agcgacagcc cgcggcgatt
     1561 gttgccgacc cgcatcgcga gccccgtatc gacatagtcg ccctggcttt cgccgagcac
     1621 cggaccgatg ccgagccgcg agggcccgat ataattcgcc cagccgttca cgcggaaatc
     1681 ctcgtcgccg atcatcgtcg cataattgac cgacccgcgc accgcatggc ttgcgacgtt
     1741 tggaatccgg ccgagccgcg cgggcgccgc ggcgaacacc ggcaggatct gcggcgacag
     1801 atcgtcgacg cggctgtggt tgtagaccgc gccgagttcg aaggtcagcg catcggtcgg
     1861 ccgcatcgcg accgcgcccg acaggctggt gatgcggccg ttgccgatat tggcggtggt
     1921 cggaaagccc gtgctgtcga tgaaatccgc ctggatgttg cgccagcggc tgtgcgagat
     1981 cgaaatgctg gcgtcgagca ggctccgccc cttgtcgccg aaccgcattc cggcttccca
     2041 ggtgcggacc ttgtcgttga ggaaacgccg cacgaaattg ccgtcgaccg cgaggccgcc
     2101 ggggcggaaa ccttcctgat agcgcgcata gagcctgaca ttgtcgagcg gggtcgccag
     2161 cagcgaaacc gacggcagca gatcggtttc gttgcgcgac gcggtcgtcg cccggttcgc
     2221 ctccgccagc gcgaaggata cgccctcggc ctcgccgccc agccgcgcgt gcgataggcg
     2281 gaggccgccc gaagcgatca ggttcggcat gatttcgacc gtcgcctcgg catagccggt
     2341 gacctcggtg atccggttgg tgacgccggg caggaccgcg cgaagcgggc cataggcata
     2401 ttcgcggtcc tgccgtgcgc ggttgttgat gaagctcgcg ccgacgaccc agccgagccc
     2461 gtcgtgatag ggccgcgaca ggcggttttc gctgacgaac atgcgcgtgt cgttatgctg
     2521 gtcgagcacg cgcgggacat cggccatggg cgtgatctcg accggggcga acaggcgctc
     2581 gacatcgacc gcgcccatgg caacgccggc atcgtccccg tcgccgcgcc gcgattcggg
     2641 caggctcgcg tcgaaccgct cgaagaggcg atggtcgatg aaggcgttcg acgactggaa
     2701 ataaaggtcg ccccaatcct tcatcacgac gatcgtgccc atgccgtaac gcgcggtcgc
     2761 attctgctcg accatcgagg cgcgggtcag cggcggcgca tccttgtcgg catattgtgc
     2821 gtcgtcggag gtgatcgcct gatagatgcc tccgaaatcg accgtccagc cgtcgccggc
     2881 ttcgaggcgg aaggtgcccc gtccgccgcg gacatggacg cggttgacgt cattctgccc
     2941 gcgcagcgga ttgtcgatat agccgccgtc ggtgagcata tagccgacga ggcgtagcgc
     3001 atggccgtcc tcgcccacgg gcaggttggc gatcgcggcg atgtcgccgc ccggatcgcc
     3061 gtgctgggtg accgacaggc cggcgatcgc ctggacgctc atcgcgcgcg gatcgggcgc
     3121 cttcggcacg acgtggatga tcccgccgag cgagcccgcg ccatagagcg tgccctgcgg
     3181 cccttcgagg atttcgacat tgtcgatgtc gtagaggcgc aggtccgggt cgggcgcgtt
     3241 gtagctgagg cggatatcgc cgaaatattg gccgacggtc gattgcgtcg ggccggtgaa
     3301 gctcgaatcg gcgataccgc ggatgaacag cttgttgcgc cccgaaccca gatgggtcga
     3361 ggaaacggtc gcggtgcgcg agaggatcga atccatcccg cgctcgccgc caaacgccaa
     3421 atcgtcgcca tccaattggg tgacgacgcc cgcgaaatgc gaatggggaa ggtcggtctt
     3481 cgacgcggtg acgattatct cgtcccgcgc cgccgcgatc atgtcgttcg ccggctcgcg
     3541 cgcccgcggc tgcggcaccg gtcgcggcac
//
LOCUS       DATMSU010000418         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_187175,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000418 DATMSU010000000
VERSION     DATMSU010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187175"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..457
                     /locus_tag="VN034_11260"
     CDS             95..457
                     /locus_tag="VN034_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61226.1"
                     /translation="MSELVREIVEVNAGIDGDEENPLLIVEVEGLAATAGWTNVRLDP
                     HAYITPPDDGVQDFDLVGDRPAPNPSGAAEALSEVEAGWEGPLEDWVVGVRVHAVDNL
                     IEDEVYEPWDEDDESEAA"
     gene            751..>1560
                     /locus_tag="VN034_11265"
     CDS             751..>1560
                     /locus_tag="VN034_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWV61227.1"
                     /translation="MTMLRDPSVKYSAFPQVPLANREWPARVTTKAPIWLSTDLRDGN
                     QSLIDPMDAEKKTRFFDLLVKAGFKEIEVGFPASGATDFDYIQNLVRSGRIPEDVTPQ
                     VLTQSRADLIRTSFDSLEGAKTAIVHVYNAVSPAWRKIVFGMEMDEIKAIAIEGAKQL
                     RDNAARLPGTDWRFEYSPETFSTAELDFSIACCEAVMEILQPTADKPIILNLPATVEA
                     ATPNIYADQIEYFAKNLPNRDAAIISLHTHNDRGTGVAAAELGLLAGADRVE"
BASE COUNT          314 a          566 c          451 g          229 t
ORIGIN      
        1 caaaaagacc gcgcaccaat ctgtcacacg caccgcgtat cagggggcgg gaccaacgct
       61 ggtcccgccc cttttcgcac aaggagccca tgtgatgagc gaactggtac gcgaaatcgt
      121 cgaagtgaac gccgggatcg acggcgacga ggaaaatccg ctgctgatcg tcgaggtcga
      181 gggccttgcc gccaccgcgg gctggaccaa cgtccggctc gacccgcatg cctatatcac
      241 cccgcccgac gacggcgtgc aggatttcga cctcgtcggc gaccgccctg cccccaatcc
      301 ttcaggcgcc gccgaggcgc tcagcgaggt cgaggccgga tgggaaggcc cgctcgagga
      361 ttgggtcgtc ggcgtgcgcg tccacgcggt cgacaatctg atcgaggacg aggtctacga
      421 accctgggac gaggacgacg agagcgaagc cgcctgaggc cgctccgcaa ccacccctgc
      481 actacgccgg gccgcgagtt ccttcgcggc ccggcgacat tttcggttca tcaccgaacc
      541 ggcaagcgcg gccgagcggc gcaattttca cactcccccc ttgccaaacg cgacattttg
      601 aaccaatagc ctgcccatgc gcggcaacgc ccttcttctt ctgcgactta cacgcccttg
      661 ggcgtgacac tggctgcgcg ctagtcgcgg ccacccgctc aagggtccga tcgacaagca
      721 ctcgcaacca ccagaagaag tttgaacccc atgaccatgc tccgcgaccc ctcggtcaag
      781 tatagcgcct tcccgcaggt tcccttggcg aaccgcgaat ggcccgcccg cgtcaccaca
      841 aaagccccga tctggctctc caccgatctg cgcgacggca accagtcgct gatcgacccg
      901 atggatgccg agaaaaagac gcgcttcttc gacctgctcg tcaaggcggg cttcaaggag
      961 atcgaggtcg gcttccccgc cagcggcgcg accgatttcg actatatcca gaacctcgta
     1021 cgcagcggcc gcatccccga agacgtcacg ccgcaggtgc tgacgcagag ccgcgccgac
     1081 ctcatccgca ccagcttcga cagcctcgaa ggcgcgaaaa ccgcgatcgt ccacgtctat
     1141 aacgcggtca gccccgcgtg gcgcaagatc gtcttcggca tggagatgga cgaaatcaag
     1201 gcgatcgcga tcgagggcgc gaagcagctc cgcgacaatg ccgcgcgcct gcccggcacc
     1261 gactggcgct tcgaatacag ccccgaaacc ttctcgaccg ccgagctcga tttcagcatc
     1321 gcctgctgcg aagcggtgat ggagatcctg cagccgaccg ccgacaagcc gatcatcctc
     1381 aacctccccg caacggtcga ggcggcgacg ccgaacatct acgccgacca gatcgaatat
     1441 ttcgccaaga acctgccgaa ccgcgacgcc gcgatcatca gcttgcacac ccacaacgac
     1501 cgcggcaccg gcgtcgcggc ggccgagctc ggcctgctcg cgggcgccga ccgcgtcgag
//
LOCUS       DATMSU010000419         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_35577, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000419 DATMSU010000000
VERSION     DATMSU010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35577"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..1001)
                     /locus_tag="VN034_11270"
     CDS             complement(84..1001)
                     /locus_tag="VN034_11270"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV61228.1"
                     /translation="MATLDLKALRHFVSVAESLSFARAAERLGTSQPVVTRTVAQLEH
                     NLGAKLFERTTRRVALTPAGVVLLKAARPLLGQAEALQRTIRHAVAVESGRLSIGTTK
                     IAMQTVAPAYIRRFTQIRPDIELVVQEFSTSAQIEALLSAEIDVGFVLLPASHSGLTI
                     EPIHSERMMLAVSDGHPCADFGLASPLPLSAFAHDRFIVPHNQGNSANHDEMVSICRQ
                     AGFRPKTLESCDDQTCMGLVRAGMGVLFAPMATQPVSTEGIRLIEIEDPVPTLKIGLA
                     WRKDDPSASLRLFRDQTRQTDAPVEQAPI"
     gene            1399..1701
                     /locus_tag="VN034_11275"
     CDS             1399..1701
                     /locus_tag="VN034_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61229.1"
                     /translation="MFKLVAIGLVGVVALGAVPASAQVFQGPYIGAQAGWNRDSVDAP
                     RTDAGIATINDEKDSFIGGAFVGYNQTVTPNIVLGLEGSFNVGASDDIRRNCSGSQ"
     gene            complement(2009..3352)
                     /locus_tag="VN034_11280"
     CDS             complement(2009..3352)
                     /locus_tag="VN034_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWV61230.1"
                     /translation="MMGRQVAQGALFYGFRLDDHVPVDHLLRRIDGVLDFGFVREALA
                     SSYSTSGRPSIDPELMLRMLLVGYLFGIRSERRLCEEVHLNLAYRWFCRLDLGDRVPD
                     HSTFSKNRHGRFRACDLHRLLFEQVVARCAAAGLVTGRDLAVDGSTIMADASREKKLK
                     GADAADELRAGESISRPVAEYLAALDAALPPDPDEPATVSPTSISPTDPQAALTCKHG
                     PALYAYAINPLLDLDTDCILDVEATPARFSSEVAATRTLVSRAGATLGITPESLAADK
                     AYGSGPLLDWLIDRKITPYIPVIDRSRQRDAFFTRDAFRYDREADAYRCPADKPLGYC
                     GTKRVSQVRVYRSHVADCAGCELKPRCTVSRKRSITRLVSEDARDTVRALAGTEAYVH
                     ARRQRQRIERVFGHLKRNLGLRTLKLRGLAGAAEEFTMAAAAYNLQLLARQTAPA"
BASE COUNT          643 a         1042 c         1102 g          645 t
ORIGIN      
        1 gtcccagatc ggccaatgac cgaacgttca cgctttgagc gatcatgcga gattgccacg
       61 gtccggatcc ggatccgccc taatcaaata ggtgcctgct ccactggcgc gtcagtttgg
      121 cgggtctggt cgcgaaataa ccggagggac gctgatgggt cgtccttgcg ccacgccaat
      181 ccgatcttaa gcgtcgggac aggatcctcg atttcgatca gccttatccc ttcagtagaa
      241 accggctgcg tggccatagg cgcgaacagc acgcccatgc ctgctcgcac gagacccatg
      301 caggtctgat cgtcgcagct ctcaagcgtc ttgggacgaa aaccggcctg cctgcagatc
      361 gagaccatct cgtcatggtt cgcggaattg ccctggttat ggggcacgat gaagcgatca
      421 tgggcgaaag ccgacagcgg caagggcgag gcgagaccga aatcggcgca tggatgaccg
      481 tcggatacgg ccagcatcat ccgttcgctg tggattggct cgatcgtcag gccggagtga
      541 gacgcaggaa gcaggacgaa cccgacgtcg atctccgccg agagcaatgc ttcgatctgg
      601 gcggatgtgg agaattcctg gaccacaagt tcgatgtccg ggcgaatctg cgtgaaccga
      661 cgtatatagg caggcgctac ggtctgcatc gcgatcttcg ttgtaccgat agacaggcgc
      721 ccgctctcaa cggccacggc atgtctgatc gtcctctgga gcgcttcggc ttgtccaagg
      781 aggggccttg ccgccttcaa aaggacaacc cctgcaggcg ttaaagcgac acggcgcgtg
      841 gtacgctcga acagcttggc tccgagattg tgctcgagtt gcgcaacggt gcgggtcacg
      901 acgggctggg acgtgccaag gcgctccgcg gcgcgggcaa agctcagcga ttccgccaca
      961 ctcacgaaat gtcgcagcgc cttgagatcc agtgtcgcca tcgtttgtcc actttttcgg
     1021 gcactatgat taatgcgaaa ttgttattaa ttctattgcc atcttagaaa ttatcttcgg
     1081 cgatcccctc cctatatcct cgaaatagga gctgcttccc ggctctggac aggtagcgcg
     1141 gaacgctccg gtccgggtcc tggacagaga cttttgggct tgctgcccgg taatgcatcg
     1201 cgtcgttctt cagtgaccca agcgggcccc agcccagttg tggacgtgaa gacgcgcgta
     1261 tcggccgcag cgaccccagc cactgcgtgc atgagccctg gttcatgcac cgaacccgtc
     1321 tgacccgcgc gcgccggcgg ttcaacatcg gaccatcgcg gggcgcgcta gaaatgttca
     1381 ggaagcaagg aaatcgtcat gttcaagttg gtcgcaattg gcttggttgg ggtcgtcgcg
     1441 ctgggtgcag tccccgcctc ggcacaggtt ttccaggggc cctacatcgg cgcgcaagcg
     1501 ggctggaatc gggacagcgt cgatgctccc cggaccgatg ccggcatcgc tacgatcaac
     1561 gacgagaagg acagcttcat cggcggcgcg ttcgtgggct ataatcagac cgtgacgccc
     1621 aatatcgtgc tgggcctcga gggaagcttc aacgtcggag ccagcgacga tatccggcgt
     1681 aattgtagcg gctcgcaatg atgatcgccc ggaactgcaa tatagggcgg ttttcggctc
     1741 gcattgcgtg agacggatcg atgcggggaa cgcacgctcc gtgagacaga aaccacattc
     1801 ggtgcgaccg acgcgcaatg ctgatccgtt ggcttacgga cggcaaaaag cggtggttag
     1861 cgcccgggca gctttccgag ccggcatggt cgaataccgc cgccctccct ccgagggtca
     1921 taaccgttcg agaccgttga aaaaggcctg cgcgaaatcg gcgtggcgga tggctggtga
     1981 cgccggacgt cggcggatgc gccgcgtatc aggccggcgc ggtctgccgg gccagcagct
     2041 ggaggttgta tgccgcggca gccatggtga actcttcggc tgctccggcg aggcctcgca
     2101 gcttgagcgt tcgtagcccc aagttgcgtt tcaggtggcc gaacacgcgc tcgatccgct
     2161 gtctttgacg ccgcgcgtgc acataggctt cggtgccggc aagggcgcgc acagtatcgc
     2221 gagcatcctc gctgacgagg cgggtgatac tgcgtttccg gctgacggtg catcggggct
     2281 tcagttcgca gccagcacaa tcggcaacat ggcttcggta tacgcgaacc tggctgacgc
     2341 gtttggtgcc gcaatagcca agcggcttgt ccgccgggca gcgataggca tcggcctcgc
     2401 gatcatagcg aaaggcatca cgagtgaaga aggcgtcgcg ctggcggctg cggtcgatga
     2461 ccggaatgta cggggtgatt tttcggtcga ttagccagtc gagcaacggg ccgctgccat
     2521 acgctttgtc agccgcgagg ctctcgggtg tgatgcccag cgtggcgccg gcacgcgaca
     2581 ccagtgtccg ggtcgccgcg acctcggacg agaagcgcgc tggcgtcgcc tctacatcga
     2641 ggatgcagtc ggtatcgagg tcgagcagcg ggttgatcgc gtatgcgtag agcgccggcc
     2701 cgtgcttgca ggtgagcgcc gcctgcgggt cggtcggcga gatgctggtc gggctgacgg
     2761 tcgcaggttc atcgggatct ggcggcaacg cggcatcgag cgccgctaga tattcggcga
     2821 cgggtcggga tatgctttcc ccggcacgaa gctcgtctgc tgcatcggct cccttcagct
     2881 tcttctcgcg gctggcgtcg gccatgatcg tgctgccgtc caccgccaag tcgcgccccg
     2941 ttaccagccc ggccgcggcg caccgcgcga ctacctgctc gaatagcaga cgatgtaggt
     3001 cgcaggcgcg gaagcggccg tgccggttct tggagaaggt ggaatggtcg ggcactcggt
     3061 cgcccagatc gagccggcag aaccaacgat atgcgaggtt gaggtgcacc tcctcgcaca
     3121 ggcggcgttc cgagcggatg ccgaacagat agccaaccag cagcatcctg agcatcagct
     3181 ccgggtcgat cgagggtcgc ccactcgtgc tatagctcga cgccagcgcc tcgcgcacga
     3241 agccgaagtc caacacgccg tcgatgcgtc ggagcaggtg gtcgaccggc acatgatcat
     3301 cgagccggaa accatagaac agcgccccct gcgccacctg ccgacccatc atgtccgtga
     3361 tccccctacc gccttgcagg agtgaatcac gtcctcgtca ccgtcgcaag cagccgtttt
     3421 tcaacagcct cg
//
LOCUS       DATMSU010000420         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_130677,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000420 DATMSU010000000
VERSION     DATMSU010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130677"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VN034_11285"
     CDS             <1..316
                     /locus_tag="VN034_11285"
                     /inference="COORDINATES: protein motif:HMM:NF025668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HWV61231.1"
                     /translation="GEALPVKLTLRNDGKRAGKEVAQLYVAPVTPAAGDAPIALRGFA
                     KADLAPGARRTLTVTLDPRSFSKFDAERGEWVVVPGRYRIMAGASSADIRQQRDIEVT
                     AD"
     gene            complement(317..1279)
                     /locus_tag="VN034_11290"
     CDS             complement(317..1279)
                     /locus_tag="VN034_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWV61232.1"
                     /translation="MAGGLAIVLSGGGAKGAFQVGVVHELVVNRGVQLDIVAGVSTGA
                     IQALGVAQDDVPALLDAWLGIRGNSSIYKSRPLGVVGGLLGEDALYDTGPLKRLLKGF
                     ANEAKLRASGRKLLLGVVNLGTGTYRTIDESVPGIHNWVYASCAMPLFFDPLKTRASD
                     GTEEQWVDGGVRDVTPLSSALDMNPRGVIAVRASPAPQPGAVRTFDNLIEIGLRAVDI
                     LQSEVSANDLAGAALINDLIAAREAQLRALQSEGIGGAQAARILRPLDVQISRYRFAP
                     IRIIEPEEEYSETLDFDPAKIRTAIDAGRRAVEREWDALEPLLS"
     gene            1440..2975
                     /locus_tag="VN034_11295"
     CDS             1440..2975
                     /locus_tag="VN034_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV61233.1"
                     /translation="MSDAFGSNNRPTSADVAIIGAGPAGLTAAYLLTQQGYKVTVIEK
                     DPVYVGGISRTVEHEGFRFDIGGHRFFSKSREVVELWNEILPHDFIERPRMSRIYYEG
                     RFYSYPLRAFEALWNLGVVRSTLCMMSYAKAKLLPKKTVRSFEDWTINQFGQKLYSIF
                     FKTYTEKVWGMPCDEMSADWAAQRIKGLSLGGAVIDGLKRSLGLNKRPNDGMATKTLL
                     ETFRYPRLGPGMMWEAARDKVVAGGNHVLMGHSFKQLTQDQKTGRWRMTASGPDGEVV
                     IDAGHVISSAPMRELAARIHPLPTCALTAAPKLGYRDFLTVALKIRSEDLFPDNWIYI
                     HDNKVQVGRVQNFRSWSPEMVPDPAIACVGLEYFCFEGDGLWSSSDERLIALATKEME
                     ILGLCDPEDVVGGAVVRQEKAYPVYDDDYAAHVETMRSELEARYPTLHMVGRNGMHRY
                     NNQDHAMMTAMLTVRNIVAGEKLYDIWGVNEDAEYHESGTEGEQAALASVRDVPARVA
                     KAA"
BASE COUNT          539 a          938 c         1039 g          528 t
ORIGIN      
        1 cggcgaggcg ctgccggtca aactcacgct tcgcaacgac ggcaagcgcg ccggcaaaga
       61 agtggcgcag ctttacgtcg cgccggtaac cccggcggca ggcgacgcgc cgatcgccct
      121 tcgcggtttt gccaaggctg atctcgcgcc gggtgcgcgc cggaccctga ccgtgacgct
      181 cgatccccgg tccttctcga aattcgatgc tgagcgcggc gaatgggtcg tcgttcccgg
      241 ccggtaccgg atcatggcgg gggcgtcgtc ggcggatatc cgccagcagc gcgacatcga
      301 ggtgaccgcc gactgatcag ctgagcagcg gttcgagcgc gtcccattcg cgctcgaccg
      361 cgcgccggcc ggcatcgatc gcggtgcgga tcttcgcggg atcgaagtcg agcgtttccg
      421 aatattcctc ctcgggctcg atgatccgaa tcggcgcaaa gcgatagcgc gagatctgga
      481 cgtcgagcgg gcgcaggatg cgcgccgctt gcgcgccccc gatgccctcg ctctggagcg
      541 cgcgcagctg cgcctcgcgc gcggcgatga ggtcgttgat cagcgcggcg ccggcgagat
      601 cgttcgccga gacttcggat tgcaggatgt cgaccgcgcg cagcccgatc tcgatgagat
      661 tgtcgaaggt gcgcaccgcg ccgggttgcg gcgcgggcga ggcgcgtacc gcgattacgc
      721 cgcgcgggtt catatcgagc gccgagctca agggggtgac gtcgcgcacg ccgccgtcga
      781 cccattgctc ctcggtcccg tcgctcgcgc gcgtcttcaa gggatcgaag aagagcggca
      841 tcgcgcagct cgcatagacc caattgtgga tgccgggcac gctttcgtcg atcgtgcggt
      901 aggtgccggt gccgagattg acgacgccga gcaggagttt gcgcccgctc gcgcgcagct
      961 tcgcctcgtt cgcgaaaccc ttgagcaggc gcttcaacgg tccggtgtcg tagagcgcgt
     1021 cctcgccgag cagcccgccg acgacgccga gcgggcgcga tttatagatc gagctgttgc
     1081 cgcggatgcc aagccaggcg tcgagcagcg cggggacatc gtcctgcgcg acgcccaagg
     1141 cctgaatcgc gccggtcgag acgccggcga cgatgtcgag ctgcacgccc cgattgacga
     1201 caagttcatg gacgacgccg acctgaaagg cgcccttcgc gccgccgccg ctcaatacga
     1261 ttgccagacc gcctgccatg tgcgcctcct tggcctacgc cagtttacag caagatggcg
     1321 ggcttcccaa ggtgcgaagc cgcgtttccg cgagaatccc gccgccgcgt taaccagttt
     1381 ttatccatga ttggccacag gggtagcgag catgaatttg cctgtgggga tcaatcaata
     1441 tgtccgacgc ttttggttcg aacaatcgtc cgacgagcgc cgacgtcgcc atcatcggcg
     1501 cgggaccggc ggggctgacc gccgcctatc tactgacgca gcagggctat aaagtgacgg
     1561 tgatcgagaa ggatcccgtc tatgtcggcg gaatcagccg cacggtcgag catgaggggt
     1621 ttcgtttcga tatcggcggg catcgcttct tttcgaagag ccgcgaagtc gtcgagctgt
     1681 ggaacgagat tcttccgcat gatttcatcg agcgtccgcg gatgagccgc atctattatg
     1741 agggcagatt ctactcctat ccgctgcgcg cgttcgaggc gctgtggaac ctcggcgtcg
     1801 tgcggtcgac gctgtgcatg atgagctatg ccaaggcgaa gctgctgccg aagaagacgg
     1861 tgcgcagttt cgaggactgg acgatcaacc agttcgggca gaagctttat tcgatctttt
     1921 tcaagaccta taccgagaag gtgtggggca tgccgtgcga cgaaatgtcg gccgactggg
     1981 cggcgcagcg catcaagggg ctgagcctcg gcggcgcggt gatcgacggg ctgaagcgca
     2041 gcctcgggct caacaaaagg cccaacgacg gcatggcgac caagaccctg ctcgaaacct
     2101 ttcgttatcc gcggcttggc ccggggatga tgtgggaagc ggcgcgggac aaggtcgtcg
     2161 cgggcggcaa ccatgtgctg atgggccata gcttcaagca gctgacgcag gaccagaaga
     2221 ccggccgctg gcggatgacc gcgagcggac ccgatggcga ggtcgtgatc gacgccggcc
     2281 atgtcatcag ctcggcgccg atgcgcgagc tcgcggcgcg gatccatccg ctgccgacgt
     2341 gcgcgctcac cgccgcgccg aagctgggtt accgcgactt cctgaccgtc gcgctcaaga
     2401 tccgttcgga ggatctgttc cccgacaact ggatctatat tcacgacaac aaggtgcagg
     2461 tcggccgggt gcagaatttc cgcagctggt cgcccgaaat ggtgcccgat cccgcgatcg
     2521 cgtgcgtggg ccttgaatat ttctgtttcg agggcgacgg gctctggtcg tcgagcgacg
     2581 agcgtctgat cgcgctggcg acgaaggaga tggaaatcct ggggctttgc gatcccgaag
     2641 atgtcgtcgg cggcgcggtg gtgcggcagg aaaaggccta ccccgtctat gacgacgatt
     2701 atgccgcgca tgtcgaaacg atgcgcagcg agctcgaggc gcgttacccg acgctgcaca
     2761 tggtcgggcg caacggcatg caccgctaca acaaccagga tcatgcgatg atgaccgcga
     2821 tgctgacggt gcgcaacatc gtcgcgggcg agaaactcta tgatatctgg ggcgtcaacg
     2881 aggacgccga atatcatgag agcggcaccg agggcgaaca ggccgcgctc gcgtcggtcc
     2941 gcgacgtgcc tgcgcgcgtg gcgaaggccg cgtaagcgct tggccggggg gatcggcgcc
     3001 atgctaccgg tgaccagatt gatcgcgtcg gtgcggcgcg gcga
//
LOCUS       DATMSU010000421         6854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_45594, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000421 DATMSU010000000
VERSION     DATMSU010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45594"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="VN034_11300"
     CDS             complement(<1..139)
                     /locus_tag="VN034_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWV61234.1"
                     /translation="MAKVIGIDLGTTNSCVAVMEGGKPKVIENVEGTRTTPSIVAFAK
                     DG"
     gene            296..832
                     /locus_tag="VN034_11305"
     CDS             296..832
                     /locus_tag="VN034_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWV61235.1"
                     /translation="MKPFALAAFAAAALSLTGCGENLGAGQPLNVVSGHIVMGATADR
                     PAVGYFRVEGGPRDVELVAVTSDLAQRVEMHESVRENNVMTMKPLLRADVPAKGELVF
                     KQGGKHLMIWGINGAAVRAGKLPMAFVFTNNNNERILFDMVIKQPEATATIRMDHGAM
                     DHGSTEQGAETAPDATKK"
     gene            839..1315
                     /locus_tag="VN034_11310"
     CDS             839..1315
                     /locus_tag="VN034_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vgr related protein"
                     /protein_id="HWV61236.1"
                     /translation="MPRPSRPLTAGEIALARTVFGDAIAYDRVRVCHHKWIFFQPRHI
                     VMAPMGSLHFNPHGDLYCDDFGATSQRLQGLFIHEMTHVWQAQTRGRWYLVLMRHPFA
                     TYGYSLKPGWPLARYGLEQQAEIVRHYWLLTQGVVIGGAPGVEAYRAILPASWHTV"
     gene            1373..2725
                     /locus_tag="VN034_11315"
     CDS             1373..2725
                     /locus_tag="VN034_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWV61237.1"
                     /translation="MDGSSLVVALVALAIGALIGWLFAGRQSGGLKAERDGLSERFKS
                     AVTDLAAEAEARKAADIRLAALLSEQKARDAAHDAQIAQLKDAQAALTAQFREVGQTM
                     LGEAQKAFLERAEARFRQSEETAGQNLKALLSPVHERLQKYEEAVGKVEADRRDAFGL
                     LHGQIAAMREGTERVSSEAAKLVNALRNAPKARGRWGEQQLRNVLESCGLSEHADFQT
                     EVSVADGDGGRLRPDVVVKVPGGQSLVIDAKVSLNAYQDAFGAVDEGEKVAHLAAHAA
                     AMRAHVNTLGAKSYWNQFDDTPDFVVMFVPGEHFLAAALDQDHELWDYAFERKVLLAT
                     PTNLIAIARTVAAVWRQEKLAGQAREIAALGKELYARMSVMGSHIARVGKNLDQATGA
                     YNAFVGSFESQVLTQAKRFEALDVETGGKEIPTLPVAEQAARPLAKLAPAPAAVNDGE
                     "
     gene            complement(2878..3588)
                     /locus_tag="VN034_11320"
     CDS             complement(2878..3588)
                     /locus_tag="VN034_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HWV61238.1"
                     /translation="MIRSLPLPALALTALAACAPANDMPRGPGEPPAAYMALGTEPGW
                     TLEITPSRLNYDGDYGETKIMVPNPGARAVANGEHYVTDRLKVEVARGECSDGMSDRR
                     YRDTVTVTADGKTVKGCGGGILPPTELAGTNWTFVSIGGVPVAADRPTTLAFEADRLS
                     GSAGCNRFSGSYSVADGTLTAEPLMATKMACPGAGMTQESAFFALMRGPVSLTFPTDG
                     TLILTGSGGQTAVLKRVI"
     gene            complement(3755..4561)
                     /locus_tag="VN034_11325"
     CDS             complement(3755..4561)
                     /locus_tag="VN034_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWV61239.1"
                     /translation="MRRSTIESLSNPLIKRMRLLREKRHRRAERLFLAEGLRIATEAR
                     EAGILPQWLFLGPEGAAHPLAKALADATLAAGGEVIDTTPAILAKLSGKDNPQTVVGI
                     YAEPETTLADLDRGAAPIWLVAERLRDPGNLGTMLRTGDAVGAGGLILLGESTDPYAV
                     EAVRASMGAIFTQKLVVARWEEFLPWLRQGTGQLVATWLGDDTEDYQAVRYAAPTFIL
                     TGNESQGLPPAYAAAADVRVKMPMLGKADSLNAAVATAVMAYEVLNQRRN"
     gene            complement(4566..4844)
                     /locus_tag="VN034_11330"
     CDS             complement(4566..4844)
                     /locus_tag="VN034_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWV61240.1"
                     /translation="MNENSETVEITNQRGLHARASAKFVTFVSRLPESVSVEVEKGGS
                     RVNGTSIMGLMMLGAAKGDSITIHTRGDGADAALLKLVGLVKDSFGED"
     gene            complement(4841..5269)
                     /locus_tag="VN034_11335"
     CDS             complement(4841..5269)
                     /locus_tag="VN034_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWV61241.1"
                     /translation="MAHDKALPLLGMVLVTHGRLAEEMVCAMEHVVGPQRAIATVCIG
                     PNDNMEMRRKEIADAIRKVDEGRGVVMLTDLFGGTPSNLAISLLEAGRTEVIAGVNLP
                     MLIRLESARKTMELRAAVIAAKEAGQKYISVASEMLGVGP"
     gene            complement(5284..6222)
                     /gene="rapZ"
                     /locus_tag="VN034_11340"
     CDS             complement(5284..6222)
                     /gene="rapZ"
                     /locus_tag="VN034_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWV61242.1"
                     /translation="MSPAAESRLLLVTGLSGAGKSTVLKVLEDLGWEVVDNLPLALLE
                     TLIDAPMQRSEAGRPLAIGIDSRSRGFRPALLVRRIKDLREAGGRDIQTLFLDCAGAE
                     LERRFSETRRRHPMAEDRPAADGIAREREMMEPLRRWAEHVVDTTNYSSNDLQQEIRQ
                     RFAAADDGEPVLNILSFGFARGLPRNADLVFDMRYLRNPHWDPKLKPGTGLDADVAAY
                     VIADPAYEDSVAQIERLILTLLPRYRAEGKSYVTIAFGCTGGRHRSVHVAARVAQRLR
                     ESGYEPTLTHRNLDSAPQDGLEGRPPPAASAPGGNI"
     gene            complement(6233..6679)
                     /locus_tag="VN034_11345"
     CDS             complement(6233..6679)
                     /locus_tag="VN034_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HWV61243.1"
                     /translation="MLPSRTRTDIVNIHASCVAAGNGGVLILGNSGQGKSDLALRLID
                     RGAKLVADDRCDIWFERDRLWCRPPESLAGKLEVRGIGIIERAWTAPVPLALAVRLTD
                     RYDRMPSINQVEMVAGRPLPALLLSAFEASAPIKILLALERLAPGA"
BASE COUNT         1106 a         2245 c         2327 g         1176 t
ORIGIN      
        1 cgccatcctt cgcaaaggcg acgatcgacg gggtcgtgcg cgtgccttcg acattttcga
       61 taaccttggg ttttccgcct tccatcaccg cgacacagct gttcgtggtg ccgaggtcga
      121 tcccgatcac tttggccatt gataatacgt ccttgttgct tcttccggcg ccctgaaaag
      181 gcgcgccgtc catgggttac atgggcgcga tataggtgcg ctaatccttg gcacaagggc
      241 tttgacggct tatcgagggt gcgaatttgt cgcgttttta cggagtcctc ccaaaatgaa
      301 acccttcgca cttgccgctt ttgccgccgc cgcgctcagc ctcaccggct gcggcgaaaa
      361 tctgggtgca gggcagccgc tcaatgtcgt gagcggccat atcgtgatgg gggcgacggc
      421 ggaccgcccg gcggtcggct attttcgcgt cgagggcggc ccgcgcgacg tcgagctggt
      481 cgcggtgacc agcgacctcg cgcagcgcgt cgagatgcac gagagcgtgc gcgagaacaa
      541 tgtgatgacg atgaagccgc tgctccgcgc cgacgtcccg gcgaagggcg agctcgtgtt
      601 caagcagggc ggcaagcatc tgatgatctg gggcatcaac ggcgccgcgg tgcgcgcggg
      661 caagctgccg atggcgttcg tcttcaccaa caacaacaat gaacgcatcc tcttcgacat
      721 ggtgatcaaa cagcccgagg ccacagcgac aatccggatg gatcatggcg cgatggacca
      781 tggttcgacg gaacagggcg ccgaaacggc cccggacgct acgaagaaat aagcgcgggt
      841 gccgcgcccg tcgcgaccgc tgacggctgg cgagatcgcg cttgcccgga cggtgttcgg
      901 cgacgcgatc gcctatgacc gcgtgcgcgt ctgccaccat aaatggatct tcttccagcc
      961 gcgccacatc gtgatggcgc cgatgggcag cttgcacttc aacccgcacg gcgacctgta
     1021 ttgcgacgat ttcggcgcca cgtcgcagcg attgcaaggg ctgttcatcc acgaaatgac
     1081 gcatgtgtgg caggcgcaga cgcgcgggcg ctggtatctg gtgctgatgc gccacccgtt
     1141 cgcgacctac ggctacagcc tcaaacctgg ctggccgctc gcccgctacg gcctcgaaca
     1201 acaggccgag atcgtgcggc attattggct gctgacgcag ggggtggtga tcggcggcgc
     1261 gccgggggtt gaggcctatc gcgcgatatt gcctgcttcg tggcacacgg tgtgatggcc
     1321 cgcgagaaat agcggctagc cagacaggcc gtgttcgtcc tatgttccgc ccatggatgg
     1381 aagctcgctc gtcgtcgccc ttgttgccct cgccatcggc gcgctgatcg gctggctgtt
     1441 cgccggccgg caatcgggcg ggctcaaggc cgagcgcgac gggctttcgg agcggttcaa
     1501 gagcgcggtg accgaccttg cggccgaggc cgaggcgcgc aaggcggccg atatccggct
     1561 cgcggcgctg ctcagcgagc aaaaggcgcg cgacgccgcg cacgatgcgc agatcgcgca
     1621 attgaaggac gcgcaggcgg cgctcaccgc gcaattccgc gaagtcgggc agacgatgct
     1681 cggcgaggcg caaaaggcgt tcctcgaacg cgccgaagcg cgtttccggc agagcgagga
     1741 gacggcggga cagaatctga aggcgctcct gtcgcccgtc cacgagcgat tgcagaaata
     1801 tgaggaagcc gtcggcaagg tcgaggccga ccgccgcgac gccttcggcc tgctccacgg
     1861 ccagatcgcg gcgatgcgcg agggcaccga acgcgtgtcg agcgaggcag cgaagctggt
     1921 caacgcgctg cgcaacgccc ccaaggcacg cgggcgctgg ggcgagcagc agcttcgcaa
     1981 tgtgctcgaa agctgcggac tttccgaaca tgcagatttc cagaccgagg tcagcgttgc
     2041 cgatggcgat ggcggccggc tgcgccccga tgtcgtcgtc aaggtgccgg gcgggcagag
     2101 cctcgtcatc gacgccaaag tctcgctgaa cgcctatcag gacgccttcg gcgcggtcga
     2161 cgagggcgag aaggtcgcgc atctcgccgc gcatgccgcg gcgatgcgcg cgcacgtcaa
     2221 cacgctcggc gccaaaagct attggaacca gttcgacgac acccccgatt tcgtggtgat
     2281 gttcgtcccc ggcgaacatt tcctcgccgc cgcgctcgat caggatcacg agctttggga
     2341 ttatgcgttc gagcgcaagg tgctgctcgc gaccccgacc aacctcatcg cgatcgcgcg
     2401 caccgttgcg gcggtgtggc ggcaggaaaa gctcgcgggg caggcgcgcg agatcgcggc
     2461 gctgggcaag gagctctatg cgcgcatgtc ggttatgggc tcgcacatcg cgcgcgtcgg
     2521 caagaatctc gatcaggcga cgggcgcgta taacgctttc gtcggcagct tcgaaagcca
     2581 agtgctgacg caggcgaagc ggttcgaggc gctcgacgtc gagaccggcg gcaaggagat
     2641 tccgacgctt cccgtcgccg aacaggcggc gcgccccctc gcgaagctgg ctccggcgcc
     2701 cgcggcggtc aacgacggcg agtgagatcg tagagcgaaa ccattcgatg gaatcgtcat
     2761 cccggcggag gccgggatct cgccggtgct tcgttccgat agggtgagat cccggcctcc
     2821 gccgggatga cggaataagg aaggctgcat ttcagcctga cgattgcgct tccggctcta
     2881 aatcacccgc ttcagcaccg ccgtctgccc gccgctcccc gtcaggatca acgtcccgtc
     2941 ggtcgggaag gtcaggctca ccgggccgcg catcagcgcg aagaaggcgc tttcctgcgt
     3001 catccccgcg ccggggcacg ccatcttggt cgccatcaac ggttcggcgg tcagcgtccc
     3061 gtcggcaacc gaatagctgc ccgaaaaccg gttgcagccc gcgctgccgc tcagccggtc
     3121 ggcttcgaag gcgagcgtgg tcgggcggtc ggccgcgacg ggcaccccgc cgatcgagac
     3181 gaaggtccag ttggttcccg ccagctcggt cggcggcagg atcccgccgc cgcagccctt
     3241 caccgtcttg ccgtcggcgg tcacggtcac cgtgtcgcga tagcggcggt cgctcatccc
     3301 gtcgctgcat tcgccgcgcg cgacctcgac cttcagccgg tcggtcacat aatgttcgcc
     3361 gttcgccacc gcgcgcgcgc cggggttcgg caccatgatc ttggtctcgc cataatcgcc
     3421 gtcgtaattg agccgcgacg gcgtgatctc gagcgtccag ccgggctcgg tgccgagcgc
     3481 catataggcg gcgggcggtt cgccggggcc gcgcggcatg tcgttcgcgg gcgcgcaggc
     3541 ggcgagggcg gtcagggcga gggcgggcag gggtagagag cggatcatcg aacagccttt
     3601 cctttctcgt catgctgaac ttgtttcagc atccatggcc cgccttctcg tgccgcgcgg
     3661 cgcttggggg ccgggcaggc catggaccct gaaacaagtt cagggtgacg agggatttat
     3721 gaagcaccga atgcaggacg ccgaaccgca gctatcagtt ccgccgctgg ttcagcacct
     3781 cataggccat caccgcggtc gcgaccgcgg cgttgaggct gtcggccttg cccagcatcg
     3841 gcatcttgac ccgcacgtcg gcggcggcgg cataggcggg cggcagccct tgcgattcat
     3901 tgccggtgag gatgaaggtc ggcgcggcat agcgcaccgc ctgataatcc tcggtatcgt
     3961 cgccgagcca ggtcgcgacg agctgcccgg tgccctgacg cagccagggc agaaactcct
     4021 cccagcgcgc gaccaccagc ttctgcgtga aaatcgcccc catgctcgcg cgcaccgcct
     4081 cgaccgcata gggatcggtg ctctcgccca agaggatcag cccgcccgcg ccgaccgcgt
     4141 cgccggtgcg cagcatcgtg ccgagattgc ccggatcgcg gaggcgttcg gcgacgagcc
     4201 agatcggcgc ggcgccccgg tcgagatcgg cgagcgtcgt ttcgggctcg gcgtagatgc
     4261 cgacgaccgt ctgcggattg tccttgcccg acagtttcgc aaggatcgcc ggcgtggtgt
     4321 cgatcacctc gccccccgcg gccaaggtcg cgtcggccag cgccttcgcc agcggatgcg
     4381 ccgcgccctc ggggccgaga aacagccatt gcggcaatat tcccgcctcg cgcgcttcgg
     4441 tcgcgatgcg cagcccttcg gcgaggaaaa gccgctcggc gcggcggtgg cgcttctcgc
     4501 gcagcagccg catccgcttg atcagcgggt tcgacaggct ctcgatggtg gaacggcgca
     4561 tgccgtcaat cctcgccgaa gctgtccttg accagcccga cgagtttcag cagcgccgcg
     4621 tcggcgccgt cgccgcgcgt gtggatcgtg atcgaatcgc cctttgcggc gccgagcatc
     4681 atcaatccca tgatcgaggt gccgttgacg cggctgccgc ccttttcgac ctcgaccgac
     4741 acgctttcgg gcaggcggct gacgaaggtg acgaatttgg cgctggcgcg cgcgtgcagg
     4801 ccgcgctggt tggtgatttc gaccgtctcg ctattttcgt tcaagggcct actcccagca
     4861 tctccgacgc gaccgagata tatttctggc cggcttcctt cgccgcgatc accgcggcgc
     4921 ggagttccat cgtcttgcgc gcgctttcga ggcggatcag catcggcagg ttgacgccgg
     4981 cgatcacttc ggtgcgcccg gcttcgagca ggctgatcgc gaggttcgac ggtgtgccgc
     5041 cgaacaggtc ggttagcatc acgacgccgc ggccctcgtc gaccttgcga atcgcgtcgg
     5101 cgatttcctt gcggcgcatt tccatattgt cgttgggacc gatgcacacc gttgcgatag
     5161 cgcgttgcgg accgacgaca tgctccatcg cgcacaccat ttcctcggca aggcggccgt
     5221 gggtgacgag caccattccc agaagcggca aagccttatc gtgcgccatg cagtcgtcga
     5281 cccttatatg ttgccgccgg gcgcggaagc cgcgggcggg ggcctgccct caagcccatc
     5341 ttgcggggcg gagtcaagat tgcggtgggt gagcgttggc tcatatccgg actcgcgtag
     5401 cctttgggca acacgtgctg cgacatggac cgagcggtgc cgcccgccgg tgcaaccgaa
     5461 cgcgatggtg acgtagcttt tgccctcggc gcggtagcgc ggcagcagcg tcaggatcag
     5521 ccgctcgatc tgcgcgacgc tgtcttcata ggcggggtcg gcgatcacat aggcggcgac
     5581 gtcggcgtcg agcccggtgc cgggcttgag cttcgggtcc cagtgcggat tgcgcagata
     5641 gcgcatgtcg aacaccaggt cggcgttgcg cggcagaccg cgcgcgaaac cgaagctcaa
     5701 tatgttgagc accggctcgc cgtcgtccgc ggcggcgaag cgctggcgga tttcctgctg
     5761 caggtcgttg ctgctgtaat tggtcgtgtc gacgacatgc tcggcccagc ggcggagcgg
     5821 ctccatcatc tcgcgctcgc gcgcgatgcc gtcggcggcg gggcggtcct cggccatcgg
     5881 gtggcggcgg cgcgtttcgg aaaagcggcg ctcgagctcg gcgccggcgc agtcgagaaa
     5941 cagtgtctgg atgtcgcggc cgcccgcttc gcgcaaatcc ttgatccggc gcacgagcag
     6001 agcgggacgg aagcctcggc tacgactgtc gatgccgatt gcgagcgggc gtccggcttc
     6061 gctgcgctgc atcggcgcgt cgatcagcgt ttcgagcagc gcgagcggca gattgtcgac
     6121 aacctcccag ccgaggtctt cgagcacctt gagcacggtc gacttgcccg cgcccgacag
     6181 gccggtgacc agcaacaggc gcgattcggc ggccggactc atcgtcgatg catcatgcgc
     6241 cgggcgccag ccgttcgagc gcgagcagga tcttgatcgg cgccgacgcc tcgaacgctg
     6301 acaagagcag cgcgggcagc gggcggcccg cgaccatctc gacctggttg atcgacggca
     6361 tgcgatcgta acggtcggtc aggcgtaccg cgagcgcgag cggcacgggt gccgtccatg
     6421 cgcgttcgat gatcccgatg ccgcggacct ccagcttgcc cgccaggctt tcgggcgggc
     6481 ggcaccagag gcggtcgcgt tcgaaccaga tatcgcagcg atcgtcggcg accagcttgg
     6541 cgccgcgatc gatcagccgc agcgccaagt ctgacttgcc ctgtcccgaa ttgccgagga
     6601 tcagcacgcc gccattgccc gccgcgacgc agctggcatg gatattgacg atatcggttc
     6661 tggtccggct cggaagcatg gcgcatggct taccggcgac gatgcggtgc tgcaaggcga
     6721 taggatgagt gcgtcagccc gcctggtggc cgccttcgcg cgcggggagg gtgatcagca
     6781 ggctcgcgcc cggcttgccg tcgtcgcgat ccttggcgct gatcgtcccg ctatgcccct
     6841 cgacgatcgt tcgc
//
LOCUS       DATMSU010000422         2600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_7696, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000422 DATMSU010000000
VERSION     DATMSU010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7696"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="sigJ"
                     /locus_tag="VN034_11350"
     CDS             complement(<1..569)
                     /gene="sigJ"
                     /locus_tag="VN034_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HWV61244.1"
                     /translation="MTPEGVRDGVDAEDAAVSFDPLRPLLTRVAYRMLGSVADAEDVV
                     QDAFIRWLGTDRDAVREPAAFLRRTVTRLCLDQIKSARSRRETYIGPWLPDPLVEEEE
                     EDNVTLPLMLALERLSPLERAAFLLHDVFGVGFDEVAKTIDRDPAATRQLAARARTRV
                     RDARPRYKLEKEKGLEIADAFFAASRSGD"
     gene            complement(569..1027)
                     /locus_tag="VN034_11355"
     CDS             complement(569..1027)
                     /locus_tag="VN034_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWV61245.1"
                     /translation="MSKVTDPFAASPQLMKQWMAVSLAAQESLEKSLIELVKIRSSIL
                     NGCANCINMHTAEARAKGETEQRIYLLAAWQEAPVFTPRERAALAWTDALTRLSQGHT
                     QKEAREALDEHFTAEEQMNLTVMINVINGWNRIAVGFELFYEGGAPAKAA"
     gene            1166..1543
                     /locus_tag="VN034_11360"
     CDS             1166..1543
                     /locus_tag="VN034_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV61246.1"
                     /translation="MKFLGGFGEQAYALLRIVAGLLFMAHGVQKFFNFPVAFPMPLNP
                     MLHAGGIIELVAGALIVIGLFTRPAAFVASGMAAVGYWMMHGSKGLYPIVNGGETIAL
                     YCFIFLFIATRGAGIWSVDGAKK"
     gene            complement(1608..1886)
                     /locus_tag="VN034_11365"
     CDS             complement(1608..1886)
                     /locus_tag="VN034_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61247.1"
                     /translation="MRARFANQTIQFGFWRRVDPDERRSRVIQTHGIGHKGRYFAAPD
                     QPHAGWTVLGLELRIFGIECLVKVVIGNPQIKRFRAIFYPDAGDGDAT"
     gene            complement(2114..>2600)
                     /locus_tag="VN034_11370"
     CDS             complement(2114..>2600)
                     /locus_tag="VN034_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6607 family protein"
                     /protein_id="HWV61248.1"
                     /translation="FETQGGIRRWRSSWTWRPLARRDAVRGPVYDRYLSINRHQPSPD
                     GWIHWQDNTKLGTKDGKLVPIVQEYVLNTYIKYDQYDVKAADDYWVATKDYWAAVRAE
                     WDRVAATKGGIAIDEKADTGTVISGRLLEMADEVQEKKLTTAKAIAEAKKLIETNTRK
                     L"
BASE COUNT          451 a          782 c          852 g          515 t
ORIGIN      
        1 atgtcgccgc tgcgcgaggc ggcgaagaag gcgtcggcga tctcgagtcc cttctccttt
       61 tcgagcttgt agcgcgggcg cgcgtcgcgg acgcgggtgc gggcacgcgc ggcgagctgg
      121 cgcgtcgcgg caggatcgcg gtcgatcgtc ttcgcaacct cgtcaaaccc gacgccgaag
      181 acgtcgtgga gaagaaaggc cgcgcgctcc aagggggaga gccgttcgag cgcgagcatc
      241 agcggcagcg tgacattatc ctcttcctcc tcctcgacca gcgggtcggg aagccaggga
      301 ccgatatagg tctcgcggcg gctccgcgcc gacttgatct gatcgaggca gagccgcgtc
      361 acggtgcggc ggagaaaagc cgcgggctcg cgcaccgcat cgcggtcggt gccgagccag
      421 cggatgaagg cgtcctgcac cacatcctcg gcatcggcga ccgagccgag catgcggtag
      481 gcgacgcgcg tgaggagcgg gcgcagcgga tcgaaactga ccgccgcgtc ctccgcatcg
      541 acaccgtccc ggacgccttc gggggtcatc aggccgcctt cgccggcgcg ccgccttcgt
      601 aaaagagctc gaagccgacc gcgatacggt tccagccgtt gatgacgttg atcatcacgg
      661 tcaggttcat ctgttcctcc gcggtgaaat gctcgtcgag cgcttcgcgc gcctccttct
      721 gtgtgtgccc ttgcgaaagg cgggtcaagg cgtcggtcca ggcgagcgcg gcgcgttcgc
      781 gcggcgtgaa gaccggcgct tcctgccatg cggcgagcag gtagatgcgc tgctcggttt
      841 cgcccttcgc gcgggcttcg gcggtgtgca tgttgatgca gttggcgcag ccgttgagga
      901 tcgacgagcg gatcttcacc agctcgatca ggctcttctc gaggctttcc tgcgccgcga
      961 ggctgaccgc catccactgc ttcatgagct gcggcgaggc ggcaaagggg tcggttacct
     1021 tggacatatc gtgtctcctt cggggggtgt gcagatatga cgagacagga tcggccggtg
     1081 tgacatggcg tgcgaaaaaa attcgcgtcg gctggaaaat cggttagcct cctcgtctcg
     1141 acaatcggag tggggggagc aatcgatgaa gtttctgggt ggtttcggcg agcaggccta
     1201 cgcactgctg cgtatcgtcg cggggctgct gttcatggcg catggcgtgc agaagttctt
     1261 caatttcccg gtcgcctttc cgatgccgct caatccgatg ctgcatgccg ggggcatcat
     1321 cgaactcgtc gcgggcgccc tgatcgtcat cggcctgttc acgcgccccg cggccttcgt
     1381 cgcaagcggc atggcggcag tcggctattg gatgatgcat gggagcaagg ggctgtaccc
     1441 gatcgtcaac ggcggcgaga cgatcgcgct ctactgcttc atcttcctgt tcatcgcgac
     1501 gcgcggcgcg ggaatctgga gcgtcgatgg ggcgaagaaa taggcttcgg caagactata
     1561 tgcaaaggtc attcctgtgg ggtctggtgc tgggtgcaac gctggcgtta ggtggcgtcg
     1621 ccatcgcccg cgtctggata aaagatcgcc ctgaaccgct ttatctgcgg attgccgatg
     1681 acaactttca cgagacattc gatgccgaaa atccggagtt ccaacccgag gacggtccaa
     1741 ccagcatgag gttgatcagg ggctgcaaaa tagcgacctt tatgaccgat accatgggtc
     1801 tggatcacac gggatcgtcg ttcgtcagga tcgactcgac gccaaaagcc aaactgaatt
     1861 gtctgattgg cgaagcgcgc gcgcattcga tgtcgctagc gatcacgcga ggattggaca
     1921 cgactgagat agtctgtgtt cccggttggc ccacacgatt cagagatgat cccccagctg
     1981 agccatgccc tcgcggcaac ccagacccga tattccggga acggcacaag gaaacaaccg
     2041 aagcagctca aacaaaataa ggggcggccc tttcgagccg cccccaggtg cgggcttcac
     2101 atggtgaagg tcttcagagc ttgcgcgtgt tggtttcgat cagtttcttc gcctcggcga
     2161 tggccttggc ggtggtcagc ttcttttcct ggacctcgtc ggccatttcg agaaggcggc
     2221 cgctgatcac ggtgccggtg tcggcctttt cgtcgatcgc gatgccgccc ttggtcgcgg
     2281 ccacccggtc ccattcggcg cgcactgcgg cccaataatc cttcgtcgcc acccaataat
     2341 cgtcggcggc cttcacgtca tattggtcgt actttatgta ggtgttgagg acatattcct
     2401 gaacgatcgg caccagcttg ccgtccttgg tgcccaactt ggtattgtcc tgccagtgga
     2461 tccagccgtc gggcgagggc tggtggcggt tgatcgagag atagcggtcg tagaccggac
     2521 cccgcaccgc gtcgcggcgc gcgagcggac gccacgtcca gctcgagcgc cagcggcgga
     2581 tgccgccctg cgtttcgaac
//
LOCUS       DATMSU010000423         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_148909,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000423 DATMSU010000000
VERSION     DATMSU010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148909"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VN034_11375"
     CDS             <1..932
                     /locus_tag="VN034_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWV61249.1"
                     /translation="RQPAAFVGISGIKPTYGRCSRWGIVAFASSLDQAGPMARDVKDC
                     AIMLENMAGFDPKDATSLDMAVPDWEAALSSDLKGKTVGIPKEYRLEGIDPDIDAMWD
                     GGIAMLKDAGADVVEISLPHTKYALPAYYIIAPAEASSNLARYDGVRYGLRDLPQGAG
                     LQDMYAATRADGFGPEVKRRIMIGTYVLSAGFYDAYYTQAQKVRTLIARDFEAAFASC
                     DVILAPTAPSAAFGLGEKTADPLAMYLNDVFAVPASLAGLPAMSVPAALNREGLPLGL
                     QIIGKAFDEQGVLNAGLAIEERAGFNARAEKWW"
     gene            934..2400
                     /gene="gatB"
                     /locus_tag="VN034_11380"
     CDS             934..2400
                     /gene="gatB"
                     /locus_tag="VN034_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWV61250.1"
                     /translation="MSDYRIKGETGEWEVVIGLEVHAQVTSNAKLFSGAATAFGAEPN
                     TQVSLVDAAMPGMLPVPNRECIRQAVRTGMAIEAQINTWSRFDRKNYFYADLPQGYQI
                     SQLYHPLVGEGSLTIAADEKAGIAGDKVIGIERIHVEQDAGKLMHDQHPTMSYVDLNR
                     SGVALMEIVSRPDMRSPAEAGAYVRKLRSILRYVGSCDGNMEEGSMRADVNVSVRKPG
                     DEFGTRTETKNVNSVRFVMAVIEGEAKRQVELIESGGTVVQETRLYDPDRNETRSMRS
                     KEDAHDYRYFPDPDLLPLELDDAFLAECRDSLPELPDAKRARYLGEGISAYNADVLTA
                     EVEAARWFDALLEAGAKPVAAANWVTSELFGALNRLGRDISDSPVSPAQAAELLGLVA
                     DGTISGTIAKQVFEKMLESGDGAAAIVDREGLKQTSDTGAIEGEIAKVLAANADKVEQ
                     YKGGKEALFGFFVGQTMKAMQGKANPQVVNELLKKALG"
     gene            2486..2974
                     /locus_tag="VN034_11385"
     CDS             2486..2974
                     /locus_tag="VN034_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61251.1"
                     /translation="MMMKRMMVSVGAALAMMAGGATSAAEAPAVNDGYDWAMRVNDEG
                     PVHDAILAYEVADTDDQPLNFTCEEGGDRVFAGISGGAPDLTAIELESGDQRVRLTGT
                     TEADEMPTFTAQEMPATFPFFRAFAANGWLRMTADGGAVDMAATASGKQAIARFAAFC
                     AG"
     gene            2979..>4071
                     /locus_tag="VN034_11390"
     CDS             2979..>4071
                     /locus_tag="VN034_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV61252.1"
                     /translation="MRYMLRALLTLWLMLAGAALARAETVVFTDVNVVPMDTERVIPR
                     TTVIVTDGVISSIGIKAKLPAGTKVIDGKGAWLMPGLADMHNHVTARDDLTLLLANGV
                     TAMLNMGEATNAFNGRTRIAVNKGEVPGPQVFTAFVVDGDPQYGHFVVRTADEGRAIV
                     RLAKTNGYSFMKVYTNLSPDAFAGIADEAQRQGIGVVGHNVKAVGLAKQLAAGQAMVA
                     HVEEFFYGFFPEPPADDQNAPPADARIAEAIALVTTHGAFVTSDLFNCRTIAAQFGKP
                     EVVKAYLAAPEARYLSPADRLAWAGSGYQKKAVDLSRRVSFEARFVKAMADAGVPLIT
                     GGDAPTIPGQVPGFALHEEIDAMLSAGLTP"
BASE COUNT          722 a         1192 c         1476 g          681 t
ORIGIN      
        1 tccgccagcc cgcggcgttc gtggggatta gcgggatcaa gccgacctat ggccgctgct
       61 cgcgctgggg catcgtggcg ttcgcgagct cgctcgacca ggcggggccg atggcgcgcg
      121 acgtcaagga ttgcgcgatc atgctcgaga atatggcggg cttcgatccg aaggatgcga
      181 cgagcctcga catggcggtg cccgattggg aagcggcttt gtcgagcgat ctcaagggca
      241 agacggtcgg tattcccaag gaatatcgat tggagggcat cgatcccgac atcgacgcga
      301 tgtgggatgg cgggatcgcg atgctgaagg atgcgggggc ggacgtggtc gagatcagcc
      361 tgccgcacac caaatatgct ttgccggctt actacatcat cgcgccggcc gaggcgtcgt
      421 cgaacctcgc gcgttatgat ggcgtgcgtt atggtttgcg cgatctgccg cagggtgcgg
      481 ggttgcagga catgtacgcc gcgacgcgcg ccgacggctt tgggcccgag gtcaagcgcc
      541 gcatcatgat cggcacctat gtgctgtcgg cgggtttcta cgacgcctat tatacgcagg
      601 cgcagaaggt gcggacgctg atcgcgcgcg atttcgaggc tgcgtttgca agctgcgacg
      661 tgatcctcgc gccgaccgcg ccgtcggcgg cgttcgggct gggcgaaaag accgccgatc
      721 cgctggcgat gtatttgaac gacgtgttcg cggtgcccgc gagccttgcg ggactgcccg
      781 cgatgtcggt gcccgcggcg ctgaaccgcg aagggctgcc gctgggcttg cagatcatcg
      841 gcaaggcgtt cgacgagcag ggcgtgctca acgccgggct ggcaattgaa gagcgcgcgg
      901 gcttcaacgc gcgcgcggag aagtggtggt aagatgagcg attatcgcat caagggcgaa
      961 accggcgagt gggaggtcgt gatcggcctc gaggtccatg cgcaggtcac ctccaacgcc
     1021 aagctgttct cgggcgccgc aacggcgttc ggggccgagc cgaacacgca ggtgtcgctg
     1081 gtcgatgccg cgatgccggg gatgctgccg gtgccgaacc gcgagtgcat ccggcaggcg
     1141 gtgcgcacgg gcatggcgat cgaggcgcag atcaacacat ggtcgcggtt cgaccgcaag
     1201 aattatttct acgccgacct gccgcagggc tatcagattt cgcagcttta tcatccgctt
     1261 gtcggcgaag gttcgctgac gatcgcagcc gatgagaagg cgggaatagc cggcgacaag
     1321 gtgatcggga tcgagcgcat ccatgtcgag caggacgcgg gcaagctgat gcacgaccag
     1381 catccgacga tgtcctatgt cgatctcaac cgatcgggcg tcgcgctgat ggagatcgtc
     1441 tcgcgccccg acatgcgttc gcccgccgaa gcgggcgcct atgtccgcaa gctgcgctcg
     1501 atcctgcgct atgtcggctc gtgtgacggc aatatggaag agggctcgat gcgcgccgac
     1561 gtgaacgtgt cggtgcgcaa gcccggcgac gaattcggga cgcgcaccga gacgaagaat
     1621 gtcaattcgg tgcgcttcgt gatggcggtg atcgagggcg aggcgaagcg ccaggtcgag
     1681 ctgatcgaaa gcggcggcac ggtggtgcag gaaacgcggc tctacgatcc cgaccgcaat
     1741 gagacgcgat cgatgcggtc gaaggaagat gcgcatgatt atcgctactt ccccgatccc
     1801 gacctgctgc cgctcgaact cgacgatgcg tttctggccg agtgccgcga cagtcttccc
     1861 gaactgcccg atgccaagcg cgcgcgctat ctgggcgagg gcatctcggc gtataacgcc
     1921 gatgtgctga ccgccgaggt cgaggcggcg cgctggttcg atgcgcttct ggaggcaggc
     1981 gcgaagccgg tcgcggcggc gaattgggtg acgagcgaat tgttcggcgc gcttaatcgt
     2041 ctgggacgcg acatttcgga ttcgccggtt tcgcccgcgc aggcggccga gctgctcggg
     2101 ctcgtcgccg acggcacgat ttcggggacg atcgcgaagc aggtgttcga aaagatgctc
     2161 gaaagcggcg acggcgccgc ggcgatcgtc gaccgcgagg ggctcaagca gacgagcgac
     2221 accggcgcga tcgagggcga aatcgccaag gttctcgccg ccaatgccga caaggtcgag
     2281 cagtataagg gcggcaagga agccttgttc ggcttcttcg tcgggcagac gatgaaggcg
     2341 atgcaaggca aggcgaaccc gcaggtcgtc aacgaattgc tgaagaaggc gctcggctga
     2401 cccgataagt gatcgcgttc acagcgaatc tggccgcttc gctgtcatgg cattgaaatc
     2461 agtgcagata tgataggaag agcggatgat gatgaagcgg atgatggttt cggtcggcgc
     2521 cgcgctggcg atgatggcgg gcggcgcgac gagcgcggcg gaagcgcctg cggtcaatga
     2581 cggctatgat tgggcgatgc gcgtcaacga cgaggggccg gtgcacgatg cgatcctggc
     2641 ctacgaagtc gcggacaccg acgaccagcc gctgaacttc acctgcgagg aaggcggcga
     2701 ccgcgtcttt gccgggattt cgggcggcgc gcccgacctg acggcgatcg aactcgaatc
     2761 gggcgaccag cgcgtgcggc tgaccgggac gaccgaggcc gacgaaatgc cgacctttac
     2821 cgcgcaggaa atgcccgcga cctttccctt cttccgcgcc tttgccgcga acggctggct
     2881 gcggatgacc gcggatggcg gcgcggtcga catggcggcg acggcgagcg gcaagcaggc
     2941 gattgcgcgt ttcgccgctt tctgcgccgg ctgacgcgat gcggtacatg ctgcgcgcgc
     3001 tgctgacgct gtggctgatg ctcgccggtg ccgcgctggc gcgggcggag acggtcgttt
     3061 tcaccgacgt caacgtcgtg ccgatggaca ccgagcgcgt cattccgcgc acgacggtga
     3121 ttgtcaccga cggcgtgatt tcgagcatcg ggatcaaggc gaagctgccc gcggggacga
     3181 aggtgatcga cggcaagggc gcgtggttga tgccggggct cgccgacatg cacaatcatg
     3241 tgacggcgcg cgacgacctg acgctgctgc tcgcgaacgg cgtcacggcg atgctcaaca
     3301 tgggcgaggc gaccaacgcc ttcaacgggc ggacacggat cgcggtgaac aagggcgagg
     3361 tgccgggacc gcaggtattc accgcctttg tcgtcgacgg cgacccgcaa tatgggcatt
     3421 tcgtcgtgcg gacggccgac gaggggcgcg cgatcgtgcg gctggcgaag accaacggct
     3481 acagcttcat gaaggtttat acgaacctgt cgccggacgc attcgccggg atcgccgacg
     3541 aggcgcagcg ccagggaatc ggcgtcgtcg ggcataatgt gaaggcggtc gggctcgcga
     3601 agcaactggc ggcggggcag gcgatggtcg cgcatgtcga ggaatttttc tacggcttct
     3661 tccccgaacc gccagccgac gatcaaaacg cgccgcccgc cgacgcgcgg atcgccgagg
     3721 caatcgcgct ggtgacgacg catggcgcgt tcgtgacgtc ggacctgttc aactgtcgca
     3781 cgatcgcggc gcagttcggc aagcccgagg tggtgaaagc ctatcttgcg gcgcccgagg
     3841 cgcgttatct gtcgcccgcc gaccggctgg cgtgggcggg gtcgggctat cagaaaaagg
     3901 cggtcgacct gtcgcggcgc gtgtcgttcg aggcgcgctt cgtgaaggcg atggccgacg
     3961 ccggcgtgcc gttgatcacg ggcggcgacg cgccgacgat cccggggcag gtgccgggct
     4021 tcgcgctgca cgaggagatt gacgcgatgc tgtcggcggg gctgacgccg t
//
LOCUS       DATMSU010000424         5305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_54628, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000424 DATMSU010000000
VERSION     DATMSU010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54628"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1027)
                     /gene="nuoH"
                     /locus_tag="VN034_11395"
     CDS             complement(2..1027)
                     /gene="nuoH"
                     /locus_tag="VN034_11395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWV61253.1"
                     /translation="MGMSAEAAWALATICGILLIALPLMLAVAMIIYADRKIWAAIAL
                     RRGPNVVGPFGLLQSFADGLKVFLQETIIPSGANRGLFLIAPIITFTVALLAWAVIPF
                     NSGAVLADINVGLLYILAISSLGVYGVILSGWASNSKYPFFSAMRASAQMISYEVSIG
                     FILIGVVLFADSFNMNEIVKAQQGHGLGIVNAFGFNLLLFPLAVMFLISSLAETARAP
                     FDLTEAESELVAGYQTEYSSMSFALFWLGEYANVLLMCTLNAVLFWGGWLPPIDWAPL
                     YAVPGIIWLFAKILFFFFVFSWVKATVPRYRYDQLMRLGWKIFLPISLLWIFLISGYL
                     MLTRYGA"
     gene            complement(1176..1313)
                     /locus_tag="VN034_11400"
     CDS             complement(1176..1313)
                     /locus_tag="VN034_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61254.1"
                     /translation="MSPVHAIAGVLFVGMGYSVWRGFNPRKSEKPGPHNGWRNQDGTR
                     K"
     gene            complement(1310..3322)
                     /gene="nuoG"
                     /locus_tag="VN034_11405"
     CDS             complement(1310..3322)
                     /gene="nuoG"
                     /locus_tag="VN034_11405"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWV61255.1"
                     /translation="MPKVTVDGVELDVPQGATVLQACEMAGKEIPRFCYHERLSIAGN
                     CRMCLVEVSPGPPKPQASCALPTAEGQVIKTDSPMVKKAREGVMEFLLINHPLDCPIC
                     DQGGECDLQDQSVAYGRGATRYDENKRAVTEKYMGPIVKTVMTRCIQCTRCVRFAEEV
                     AGVEDIGAIYRGENMQITSYLERAVASELSGNVVDLCPVGALTSKPYAFEARPWELSK
                     TLGIDMMDAVGTNVRIDARGRQVLRVLPRVNDDVNEEWASDKTRHHVDGLTRRRLDQP
                     YVRVNGALQPASWAEAFAAIKAVNAGSSVAAIAGDMADCETMFAAKSLVNALGGTLLE
                     GRQTGLDYDVTSLSAVNFNTGIVEAENADVILLVGTNLRWEAPLINTRVRKAVWKKGA
                     KVFAIGPETDLTYKTEWLGDDASLVTKLPAHVLDALKGAERPMLIFGGGALSVPGVHG
                     AGLALAKSLNAIKDGWNGFNVVHFSAARMGSLMLGYGLAGGIKDVIAAKPKLAFFLGA
                     DEVDFAALPDTFKVYVGHHGDKGAHAADVILPAAAWTEKDFTTVNTEGRVQRSEKAVF
                     APGDAREDWSIFRALADALGVSLGFDSFAQCRAAMIAAVPALGVEGLADYGWTVPKLP
                     AKPEARAIPSPIKDFYLTNAICRASPTMQRCSEELIHGVDFAEAAE"
     gene            complement(3464..4753)
                     /gene="nuoF"
                     /locus_tag="VN034_11410"
     CDS             complement(3464..4753)
                     /gene="nuoF"
                     /locus_tag="VN034_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWV61256.1"
                     /translation="MLADKDRIFTNLYGFQPWNLKSAQARGDWDNTKDLMKRGQDAII
                     EEIKASGLRGRGGAGFPTGLKWSFMPKEPRADRPSFLVINADESEPGSCKDREIIRHD
                     PHKLIEGALIAGFAMRARAAYIYIRGEFIREAETLFAAVQEAYDAGLLGKNAAKSGYD
                     FDVFVHRGAGAYICGEETAMIESLEGKKGQPRLKPPFPAGAGLYGCPTTVNNVESIAV
                     VPTILRRGAPWFASFGRENNKGTKLFQISGHVERPCVVEEEMGITFRELIDKHCGGIR
                     GGWDNLLAVIPGGSSVPLVPAAEIMDAPMDFDGLKAVGSGLGTAAAIVMDKSTDVVQA
                     ISRISYFYKHESCGQCTPCREGTGWMWRVMERLRTGDADISEIDTLFDVTKQVEGHTI
                     CALGDAAAWPIQGLIKHFRPEIERRIRENGGMVEAAE"
     gene            complement(4757..>5305)
                     /locus_tag="VN034_11415"
     CDS             complement(4757..>5305)
                     /locus_tag="VN034_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWV61257.1"
                     /translation="RQRSAVMPLLDLAQRQVGAETQTQGWLPVPVIEYVAAALDMPFI
                     RAYEVATFYTMYNLVPVGRYHVQVCGTTPCLLRGSDDVMAACKNRGMVKGKTTPDGLF
                     TLTEVECMGTCTNAPMVQINDDNYEDLDYDSTVRILDALAAGEQPKAGTQLANRHTSE
                     PDGGPTTLKEMVKANHDYRKEW"
BASE COUNT          968 a         1744 c         1647 g          946 t
ORIGIN      
        1 atcatgctcc atacctcgtc agcatcagat agccggagat caggaagatc cagagaagcg
       61 agatcggcag gaaaattttc cagcccagcc gcatcagctg gtcatagcgg tagcgcggga
      121 cggtcgcctt gacccagctg aagacgaaga agaagaacag gatcttcgcg aacagccaga
      181 tgatgccggg gacggcatag agcggtgccc agtcgatcgg gggcagccag ccgccccaga
      241 agagcaccgc gttgagcgtg cacatcaaca gcacgttggc atattcgccg agccagaaga
      301 gcgcgaagct catcgacgaa tattcggtct gataccccgc gacgagttcc gattccgctt
      361 cggtcaggtc gaacggcgcg cgcgcggttt cggcgagcga cgagatcagg aacatcaccg
      421 cgagcgggaa gagcagcagg ttgaagccga aggcattgac gatgccaagc ccatggccct
      481 gctgcgcttt cacgatctcg ttcatgttga agctgtcggc gaagagcacg acgccgatca
      541 ggatgaaacc gatcgatact tcataggaga tcatctgcgc cgaggcgcgc attgccgaaa
      601 agaagggata tttcgagttc gacgcccagc ccgacaggat cacaccgtaa acgccgagcg
      661 acgagatcgc gaggatgtag agcagcccga cgttgatgtc ggcgagcacc gcgcccgaat
      721 tgaacgggat caccgcccac gcgagcagcg cgacggtgaa ggtgatgatc ggcgcgatca
      781 ggaacaggcc cctgttggcg ccgctcggga tgatcgtttc ctgcaggaac accttcaaac
      841 cgtcggcgaa cgactgcaaa aggccgaagg ggccgacgac gttcgggccg cggcgcagcg
      901 cgatcgccgc ccagatcttg cggtcggcat agatgatcat tgcgaccgcg agcatcagcg
      961 gcaacgcgat gagcaggatg ccgcaaatcg tcgcaagggc ccacgcggcc tcggccgaca
     1021 ttcccatgtc gacccatccc ttgaacggga cgtagagcat catgcgctgg aaatattcgg
     1081 tcacagcgac cacccctttt cgtcaccccg gacttgatcc ggggtccatg gatgccggat
     1141 cgagtccggc atgacgatga tattcataac gcagctcatt tccgcgttcc gtcctggttg
     1201 cgccaaccat tatgcggacc cggcttttcc gacttgcgcg gattgaaccc gcgccagacc
     1261 gaatagccca tgccgacgaa cagcacgcca gcgattgcat gtaccgggct cattccgcgg
     1321 cctccgcaaa atcgacgcca tggatcagct cttccgagca gcgctgcatc gtcggcgacg
     1381 cgcggcagat ggcgttggtg aggtagaaat ccttgatcgg cgacgggatc gcccgcgctt
     1441 cgggcttcgc gggcagcttc ggcaccgtcc agccatagtc ggcgaggccc tcaacgccga
     1501 gcgcgggcac cgcggcgatc atcgcggcgc ggcactgcgc aaagctgtcg aagccgagcg
     1561 acacgccaag cgcatcggcg agtgcgcgga aaatgctcca gtcctcgcgc gcgtcgcccg
     1621 gtgcgaacac cgccttttcg ctgcgctgga cgcggccctc ggtgttgacc gtggtgaagt
     1681 ccttttcggt ccacgcggcg gcgggcagga tgacatcggc ggcatgcgcg cccttgtcgc
     1741 cgtgatggcc gacatagacc ttgaacgtat cgggcagcgc cgcgaagtcg acctcgtcgg
     1801 cgccgaggaa gaaggcgagc ttcggcttcg ccgcgatcac atccttgatc ccgccggcga
     1861 ggccgtagcc gagcatcagc gaacccatgc gcgcggcgga gaaatgcacg acgttgaagc
     1921 cgttccagcc atccttgatc gcgttcaaag acttcgcgag cgcgaggccc gcgccgtgaa
     1981 cgccgggcac cgacagcgcg ccgccgccga agatcagcat cggccgctcg gcgcccttga
     2041 gcgcgtcgag cacatgcgcc ggcagcttcg ttaccagcga ggcgtcgtcg ccaagccatt
     2101 cggtcttgta cgtcaggtcg gtttcggggc cgatggcgaa aaccttcgcg cccttcttcc
     2161 acaccgcctt gcggacgcgc gtgttgatca gcggcgcttc ccagcgcagg ttcgtgccga
     2221 cgagcaggat cacgtcggca ttctcggcct cgacgatccc ggtgttgaaa ttgaccgccg
     2281 acaggctggt gacgtcatag tcgagcccgg tctgtcgccc ttcgagcaag gtgccgccga
     2341 gcgcgttcac cagcgacttc gccgcgaaca tcgtctcgca atcggccatg tcgcccgcga
     2401 tcgcggcgac cgacgaaccg gcattgaccg ccttgatcgc ggcaaaggct tcggcccagc
     2461 tcgcaggctg aagcgcgcca ttgacgcgga catagggctg atcgaggcgc cggcgcgtca
     2521 ggccgtcgac atggtggcgc gtcttgtcgc tcgcccattc ctcgttcaca tcgtcgttga
     2581 cgcgcggcag cacgcgcagg acctgacggc cgcgcgcgtc gatgcgcaca ttggtgccga
     2641 ccgcgtccat catgtcgatg ccgagcgtct tcgaaagctc ccacggccgc gcctcgaacg
     2701 catagggctt gctggtcagc gcaccgaccg ggcacaggtc gacgacattg cccgaaagct
     2761 cgctcgcgac cgcgcgctcg agatagctcg tgatctgcat attctcgccg cgatagatcg
     2821 caccgatatc ctcgacgccc gcgacttcct ccgcaaagcg gacgcagcgc gtgcattgga
     2881 tgcagcgggt catcaccgtc ttgacgatcg gacccatata tttctcggtc accgcgcgct
     2941 tattctcgtc atagcgcgtc gcgccgcggc cataggcgac cgactggtcc tgcaggtcgc
     3001 attcgccgcc ctgatcgcag atcgggcagt cgagcgggtg gttgatgagc aggaactcca
     3061 tcaccccttc gcgcgccttc ttgaccatcg ggctgtcggt cttgatcacc tgcccctcgg
     3121 cggtcggcag cgcgcacgac gcctgcggct tcggcgggcc gggcgacact tcgacgaggc
     3181 acatgcggca attgccggcg atcgacaggc gttcatgata gcaaaagcgc ggaatttcct
     3241 tccccgccat ctcgcacgcc tgcaatacgg tcgcgccctg cgggacgtcg agttctacgc
     3301 catctacggt aactttaggc attcacgtcc gccttaaatt cgtcatgctg aacttgtttc
     3361 agcatccatg gcccatcgct atccacaacc gctgcgctta ccgcatcgac cggccatgga
     3421 ccctgaaaca agttcagggt gacgagggaa gagaggttgt gcatcactcc gccgcctcca
     3481 ccatgccgcc attctcgcgg atgcggcgtt cgatttcggg gcggaagtgc ttgatcaggc
     3541 cctggatcgg ccaggcggca gcatcgccca atgcgcagat cgtgtggccc tcgacctgct
     3601 tggtcacgtc gaacaaggtg tcgatctcgc tgatgtcggc gtcgccggtg cgcagccgct
     3661 ccatcacgcg ccacatccag ccggtgcctt cgcggcacgg cgtgcactgg ccgcagcttt
     3721 catgcttgta gaaatagctg atgcggctga tcgcctggac gacgtcggtc gacttgtcca
     3781 tcacgatcgc ggccgcggtg ccgagccccg atccgacggc tttcaagccg tcgaagtcca
     3841 tcggcgcgtc catgatctcg gcagcgggaa cgagcggaac cgacgacccg ccggggatca
     3901 ccgcgagcag attgtcccag ccgccgcgga tgccgccgca atgcttgtcg atcaattcgc
     3961 ggaaggtgat gcccatctct tcctcgacga cgcacgggcg ctcgacatgg cccgagatct
     4021 ggaagagctt ggtgcccttg ttgttctcac gcccgaagct cgcgaaccac ggtgcgccgc
     4081 ggcgcaggat cgtcgggacg accgcgatgc tttcgacatt gttcaccgtc gtcgggcagc
     4141 cgtagaggcc cgcacccgcc gggaacgggg gcttgaggcg cggctggccc ttcttgccct
     4201 caaggctttc gatcatcgcg gtttcttcgc cgcagatgta cgcgccggcg ccgcggtgga
     4261 cgaagacgtc gaagtcgtaa cccgacttcg ccgcattctt gccgagcagc ccggcgtcat
     4321 acgcctcctg cacggctgca aacaatgtct cggcctcgcg gatgaactcg ccgcggatat
     4381 agatatacgc ggcgcgcgcg cgcatcgcga aacccgcgat cagcgcgcct tcgatcagct
     4441 tgtgcggatc gtggcggatg atttcgcggt ccttgcacga ccccggctcg gattcgtcgg
     4501 cgttgatgac gaggaagctc gggcggtcgg cgcgcggctc cttcggcatg aagctccact
     4561 tgagcccggt cgggaagccc gcgccgccgc gaccgcgcag acccgacgcc ttgatctcct
     4621 cgatgatcgc gtcctggccg cgcttcatca gatccttggt attgtcccaa tcgccgcgcg
     4681 cctgcgcgct tttgaggttc cacggctgaa agccgtagag attggtgaag atgcgatcct
     4741 tatcggccag catggcttac cattccttcc gataatcatg attggccttg accatttcct
     4801 tcagcgtcgt cgggccgccg tcgggctcgc tcgtgtggcg gttcgcaagc tgcgttcccg
     4861 ccttcggctg ctcgcccgca gcgagcgcat cgaggatgcg aacggtgctg tcgtaatcga
     4921 ggtcttcgta attatcgtcg ttgatctgga ccatcggcgc gttggtgcag gtgcccatgc
     4981 actcgacctc ggtcagcgtg aacaggccgt cgggcgtcgt cttgcccttg accatgccgc
     5041 ggttcttgca cgccgccatc acatcgtccg acccgcgcag caggcacggc gtcgtgccgc
     5101 aaacctgcac atgatagcgg ccgacgggga cgagattata catcgtatag aaggtcgcga
     5161 cctcataggc gcggataaac ggcatatcga gcgcggcggc gacatattcg atcaccggca
     5221 ccggcagcca cccctgcgtc tgcgtctcgg cgccgacctg gcgctgcgcg agatcaagca
     5281 gcggcatcac cgccgaacgc tggcg
//
LOCUS       DATMSU010000425         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_73712, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000425 DATMSU010000000
VERSION     DATMSU010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73712"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VN034_11420"
     CDS             complement(<1..625)
                     /locus_tag="VN034_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HWV61258.1"
                     /translation="MAERSFKAEVEQLKLGDGEIFEGEGILAVTKALLQAGVAYVGGY
                     QGSPISHLMDVFADASDLMAGLGVHFESSASEATAAAMLAASVNYPLRGAVAWKSVVG
                     TNVASDALSNVASGGVTGGALIILGEDYGEGSSIMQERTHAFAMKSQMWLLDPRPSLP
                     LIVDMVEKGFELSEASNTPVMLELRVRACHVTGSFVARDNRRPPLTVA"
     gene            complement(633..1412)
                     /locus_tag="VN034_11425"
     CDS             complement(633..1412)
                     /locus_tag="VN034_11425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWV61259.1"
                     /translation="MANQILQQFAAGVASGAIRTIDLTQTLSPETPTLVLPPEFGQCA
                     AFSQEEISRYDDRGVAWYWNNFTVSEHTGTHFDAPIHWITGKDLPNNAVDTVAPADFV
                     AGAVVIDISDKAAADPDYLLTVADIEAWEADHGRIPPRSWVMLRTDWSKRDVDAYTNR
                     REDGAHTPGPSAEAVQFLVDERDAHGLGVETIGTDAGQAHLLDPAYPAHTLFHAAGRY
                     GLQCLENLDRLPATGSVIVAAPLKIKGGSGSPLRVLALVAD"
     gene            complement(1479..>2133)
                     /locus_tag="VN034_11430"
     CDS             complement(1479..>2133)
                     /locus_tag="VN034_11430"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HWV61260.1"
                     /translation="ALQGIMWRLTGDARAEWLANPGLIGAARLAPGNGDVAGFNRQLD
                     RALGALHIGRQHPLEQTLRGGTQTADDLLSRDVPEIMAVRAMIEAGVERYIDALPADP
                     VHPFLCRKASGFAFSGSWSVRLHSGGHHKNHIHPEGWISAVYYVALPDAVGDGEQGWL
                     KFGETGLNLGEREQIARTVRPEPGLLVLFPSYFYHGTIPFADEDHRTTIAFDVVPTS"
BASE COUNT          365 a          697 c          707 g          364 t
ORIGIN      
        1 cggcgacggt cagcgggggg cgccggttgt cgcgcgcgac gaagctgccg gtgacgtggc
       61 acgcccgcac gcgcagttcg agcatcaccg gcgtgttcga cgcctcggac agctcgaacc
      121 ccttttcgac catgtcgacg atcagcggca ggctgggccg cgggtcgagc agccacatct
      181 gcgatttcat cgcgaaggcg tgggtgcgtt cctgcatgat cgaggaacct tcgccataat
      241 cctcgccgag gatgatcagc gcgccgccgg ttacgccgcc cgatgcgacg ttcgagaggg
      301 cgtccgatgc gacattggtg ccgaccaccg atttccacgc gaccgcgccg cgcagcggat
      361 aattcaccga tgccgccagc atcgccgccg cggtcgcctc gctcgccgag ctttcgaaat
      421 ggacgccgag ccccgccatc aggtcgctcg cgtcggcgaa cacgtccatc aaatggctga
      481 tcggcgatcc ctgataaccg ccgacatagg cgacgccggc ctggagcagc gccttggtga
      541 ccgcaagaat gccttcgccc tcgaagatct caccgtcgcc gagtttgagc tgctcgacct
      601 ccgccttgaa cgaccgctcg gccatctgcg cctcagtccg cgacgagcgc gagaacgcgc
      661 aaggggctgc ccgagccgcc cttgatcttg agcggcgcgg cgacgatcac gctgcccgtc
      721 gccggcaacc ggtcgagatt ctcgaggcat tgcaggccgt agcggcccgc ggcgtggaac
      781 agggtgtgcg cgggataggc ggggtcgagc agatgcgcct gcccggcatc ggtcccgatc
      841 gtttcgaccc ccagcccgtg ggcgtcgcgt tcgtcgacga ggaactgcac cgcctcggcc
      901 gacggtcccg gcgtgtgcgc accgtcctcg cgccggttgg tataggcatc gacgtcgcgc
      961 ttcgaccagt cggtgcggag catcacccag ctacgcggcg ggatgcggcc atggtcggcc
     1021 tcccacgctt cgatgtcggc gacggtgagc agatagtcgg gatcggcggc cgccttgtcc
     1081 gaaatgtcga taaccaccgc cccggcaacg aaatcggcgg gcgcgaccgt atcgacggca
     1141 ttgttcggca gatccttgcc ggtgatccag tgaatcggcg catcgaaatg cgtgccggtg
     1201 tgctcgctga cggtgaaatt gttccagtac caggcgacgc cgcgatcatc gtatcgcgag
     1261 atttcttcct ggctgaacgc ggcgcactgg ccgaattcgg gcggcaggac cagcgtcggc
     1321 gtttcgggcg acagcgtctg ggtgaggtcg atcgtgcgga tcgcacccga agcgaccccg
     1381 gcggcgaact gctgaagaat ctgattggcc atgctgaact cctctcgaac cggaatgtgc
     1441 aattataaac ttgcatatgc aagtattttg acggccgctc agcttgtcgg cacgacgtcg
     1501 aaggcgatgg tggtgcggtg atcctcgtcc gcgaagggga tcgtcccgtg atagaaatag
     1561 gacggaaaaa gaacgagcag gccgggttcg gggcggacgg tccgcgcgat ctgttcgcgt
     1621 tcgccaaggt tcagccccgt ctcgccaaat ttcagccagc cctgctcgcc gtcgccgacc
     1681 gcgtcgggca gcgcgacata atagacggcg ctgatccagc cttccgggtg gatatggttc
     1741 ttgtgatggc cgccgctgtg caaccggacc gaccatgaac ccgaaaaggc aaagcccgac
     1801 gccttgcgac acagaaacgg atggacggga tcggccggca gcgcgtcgat atagcgttcg
     1861 acccccgctt cgatcatcgc gcgcaccgcc attatctccg ggacgtcgcg gctcaagaga
     1921 tcgtcggcgg tctgagtgcc gccgcgcagc gtctgttcga gcggatgttg cctgccgata
     1981 tggagcgcgc ccagcgcacg gtcgagttga cggttgaagc ccgcgacgtc gccattgccg
     2041 ggcgcaagtc gggcggcgcc gatcagcccg ggattggcca gccattccgc gcgcgcgtcg
     2101 ccggtcaggc gccacattat gccttgcagc gcg
//
LOCUS       DATMSU010000426         4591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_121417,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000426 DATMSU010000000
VERSION     DATMSU010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121417"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1323)
                     /locus_tag="VN034_11435"
     CDS             complement(109..1323)
                     /locus_tag="VN034_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWV61261.1"
                     /translation="MTLRRAAIVAPIRTAVGKFGGALSSMTAGELGAVILKALVERTK
                     IDPARVDDVVFSQGYGNGEAPSIGHWSWLAAGLPLEVPGYQLDRRCGSGLQAVINAAM
                     MVQTGMSDVVVAGGVESMSNVEHYSTDIRKGVRAGNLTLHDRLTRGRLMSQPVERFGV
                     ITGMIETAENLAKDYGITREAADAYAVQSHQRAAAAWAKGLFDDELVPVEVPQKKGDP
                     VIFAHDEGYRADASMETLGKLRPLEGGVVTAGNASQQNDAAAACLVVAEDKLDELGLE
                     PIAWYHSSAAAGCDPSRMGIGPVPAVERLFARCGLGWGDIDLVELNEAFAPQVLAVLK
                     GWGWSEGDSRNEILNVNGSGISLGHPIGATGGRILANLTRELVRRDGRYGLETMCIGG
                     GQGIAAIFERAA"
     gene            complement(1328..2536)
                     /locus_tag="VN034_11440"
     CDS             complement(1328..2536)
                     /locus_tag="VN034_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWV61262.1"
                     /translation="MYDLLAGLSVIEASSFVASPTAGLYCAQMGAEVIRVDQIGGGPD
                     FRRWPVTAANDSLYWENLNRAKKSVALDLGRPEGREILQELVRATGQFVTNFPVGGFL
                     SHEKLAEGRADLITVRVMGWADGSPALDYTVNNSVGYPMLTGAGPDPVNHVLPAWDLL
                     TGAYAAFALLAAIQRRSVSGEGGEVRIPLSDVAIGTVANLGGVAEMLYTGANRPRLGN
                     AVYGLFGRDFVTRDGRRTMIVVVTPRQWANLIAALNLGDAVARIETERGVSFAADDGL
                     RFAHRDALYPLFEAAIAARDHDDLAAAFDAGGIVHSPYRTMVDAVQDPALVAGNPIFG
                     NADNPSGFVYPAAGAFATLPGQDRKPPRAAPRNGEHSEEILAERLSLSSAELARLIDA
                     GIVGVAKSGD"
     gene            complement(2732..3748)
                     /locus_tag="VN034_11445"
     CDS             complement(2732..3748)
                     /locus_tag="VN034_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWV61263.1"
                     /translation="MIRMPSLAFVAGVLLLTLSACGDDAAETDRPAAGAKVARTCAGV
                     ADTPVKLAGGTFEMGEDDVYAEEGPVRKTTVAGFWIDPHEVTNRQFAKFVAATGYVTV
                     AEKPVDPKQFQVPADQIPPDMLKAGSAVFTPPDFPSNNYADWWKYVPGANWKKPHGPT
                     GPDQVANEPVVHLAWDDMVAYAKWAGGRIPTEAEWEYAASAGERSKNVQPVEANSWQG
                     AFPNYNAESDGFKGVAPVGCYKPNANGLYDMVGNVWEVTSDFFKPGHDPADKDNPKGP
                     SENNAYDPLNPGMPSRTVKGGSYLCAPNYCQRYRPASRQGRDPGLGTSNVGFRLVYDQ
                     APKA"
     gene            complement(3941..>4591)
                     /locus_tag="VN034_11450"
     CDS             complement(3941..>4591)
                     /locus_tag="VN034_11450"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWV61264.1"
                     /translation="PPVAADRPERRPRLFWFNLLLTAVLMAGMVSGLAPLPVLIMGAL
                     AVALTVNYPRLSDQRERIAAHADNVVMVVVLIFAAGAFTGIMGGTGMTDAMARSLLAG
                     VPESFGPYLAPITSLLALPLTFVMSNDAYYFGVVPVLAQAGQTYGIPAEAIARASLMG
                     GPVHSLSPLLAPVYLACGLLGVDVADAQRFALKYAVGISLVLTSAAILTGAIPIAA"
BASE COUNT          801 a         1568 c         1479 g          743 t
ORIGIN      
        1 gttgctcgcg gtcgatcggc cgcgccgcca gccggtcggc cagcgccgcc tgcgcgacga
       61 cgtgataggc ttgcgcagct accagcgcgc cccgcagatc gatcatgctc aggcggcgcg
      121 ctcgaagatc gcggcgatgc cctgcccgcc gccgatgcac atcgtctcga gcccgtaacg
      181 cccgtcgcgg cgcaccagct cgcgcgtcag attggcgagg atgcgtccgc cggtcgcgcc
      241 gatcgggtga ccgagcgaaa tacccgatcc gttgacgttc aaaatctcgt tccggctgtc
      301 gccctccgac cagccccagc ccttcagcac cgcgagcacc tgcggcgcga aggcttcgtt
      361 gagctcgacg aggtcgatgt cgccccagcc gagcccgcag cgcgcgaaca gccgttcgac
      421 cgcgggcacc ggcccgattc ccatccggct cgggtcgcag cccgccgccg ccgagctgtg
      481 ataccaggcg atcggttcca atccgagttc gtcgagcttg tcctcggcga cgacgagaca
      541 cgccgccgcg gcatcgttct gctggctcgc attgcccgcg gtgacgacgc cgccttcgag
      601 cggacgcagc ttgccgagcg tctccatgct cgcatcggcg cgatacccct cgtcgtgcgc
      661 gaaaatgacg ggatcgccct tcttctgcgg cacctcgacc ggcaccagct cgtcgtcgaa
      721 cagccccttc gcccacgccg ctgccgcgcg ctggtggctc tgcaccgcat aggcatcggc
      781 cgcctcgcgc gtgatgccat aatccttcgc cagattctcg gcggtctcga tcatgccggt
      841 gatcactccg aagcgttcga ccggctgcga catcaaccgc ccgcgcgtca gccggtcgtg
      901 cagcgtcaag ttgcccgcgc gaacaccttt acgaatgtcg gtcgaataat gctcgacgtt
      961 cgacatcgat tcgacgccgc ccgcgacgac gacgtcggac atgcccgtct gcaccatcat
     1021 cgcggcgttg atcaccgcct gcagccccga accgcagcgg cgatcgagct gatagcccgg
     1081 cacttcgagc ggcagccccg cggcgagcca cgaccagtgg ccgatgctcg gcgcctcgcc
     1141 attgccataa ccttgcgaaa agacgacgtc gtcgacgcgc gccggatcga tcttcgtccg
     1201 ttcgaccagc gccttcaata tcaccgcgcc gagttcgccc gcggtcatcg aggacagcgc
     1261 gcccccgaac ttgccgacgg cggtacggat cggggcgacg atggcggcgc ggcgcagggt
     1321 catctggtca atctccactc ttggcgacgc cgacgatacc ggcatcgatc aggcgggcaa
     1381 gctcggcgct cgaaagcgac aggcgttccg ccaatatttc ctcgctatgc tcgccgttgc
     1441 gcggcgcggc gcgcggcggc ttgcggtcct gtccgggcaa tgtcgcaaag gcgcccgcgg
     1501 cgggatagac gaaaccgcta ggattgtcgg cgttgccgaa gatcggattg ccggcgacga
     1561 gcgcggggtc ctgcaccgcg tcaaccatgg tgcgataggg cgaatggacg atcccgccgg
     1621 cgtcgaaagc ggcggccaga tcgtcatgat cacgcgccgc gatcgccgcc tcgaacagcg
     1681 gatagagcgc gtcgcgatgc gcgaagcgca gcccgtcgtc ggccgcgaag gacacgccgc
     1741 gttcggtctc gattcgtgca accgcgtcgc caaggttcag cgcggcgatc agattcgccc
     1801 attgacgcgg ggtgacgacg acgatcatcg tgcgccgccc gtcgcgcgtc acgaaatcgc
     1861 gcccgaacag gccataaacg gcattgccga gccgcggccg gttcgcgccc gtatagagca
     1921 tttccgccac cccgccgaga ttggcgacgg tgccgatcgc gacgtcggag agcggaatcc
     1981 gcacctcgcc accttcgccg ctgacactcc gccgctggat cgccgcgagc agcgcgaagg
     2041 cggcataagc gccggtcagc aaatcccatg cgggcagcac atgattgacc ggatccgggc
     2101 ccgcgccggt gagcatcgga taaccgacgc tgttgttcac cgtatagtcg agcgcgggcg
     2161 agccgtcggc ccagcccatc acgcgcacgg taatcaagtc ggcgcgtccc tcggcgagct
     2221 tctcatgcga caggaagccg ccgacgggaa aattggtgac gaactgcccc gtcgcgcgca
     2281 ccagttcctg caagatctcg cgcccctcgg gccggccgag gtcgagcgcg accgactttt
     2341 tcgcgcggtt cagattttcc caatagagcg agtcattggc cgcggttacc ggccagcgcc
     2401 ggaaatcggg gccgccgccg atctggtcca cgcggatcac ttcggcgccc atttgcgcgc
     2461 agtaaagccc ggcggtcgga gaggcgacga aggaagaggc ctcgatcacc gacaggccgg
     2521 ccagcaaatc atacatccgg cgcctgtctc cccaaggtca ccggcccgag cggccggtcg
     2581 aagctatctg gcgtactcga aggggtccga acccctggcc tctgccttcg gggtcggtac
     2641 taacagtccg cgccattcta ggccagagcc tgtttttctg acacaagccc ttatcattgc
     2701 gatataaaat caaaaagggt gcgtcagcat atcaggcctt cggcgcctgg tcgtaaacga
     2761 ggcggaagcc gacattgctg gtgccgagcc ccggatcgcg cccctgccgc gaggcggggc
     2821 ggtaacgctg gcaataattg ggggcgcaga gatagctgcc gcccttcacc gtgcgcgacg
     2881 gcatgccggg attgagcggg tcataggcat tgttttcgct gggccccttg ggattgtcct
     2941 tgtcggcggg atcgtggcca ggcttgaaga aatccgaggt cacctcccag acattgccga
     3001 ccatgtcgta gaggccgttg gcgttgggct tgtaacagcc gacgggcgcg acgcccttga
     3061 agccgtcgct ttcggcattg tagttcggaa aggcgccctg ccagctgttc gcttccaccg
     3121 gctggacatt cttcgaccgc tcgcccgcgc tcgcggcata ttcccattcg gcctcggtcg
     3181 gaatgcgccc gcccgcccat ttggcatagg cgaccatgtc gtcccacgcg aggtggacga
     3241 cgggttcgtt ggcgacctga tcgggaccgg tcgggccatg gggctttttc cagttcgcgc
     3301 cggggacata tttccaccag tcggcataat tgttcgaggg gaaatcgggc ggcgtgaaca
     3361 ccgccgatcc cgccttcagc atatcgggcg ggatctgatc ggcgggcacc tggaactgct
     3421 tcggatcgac cggcttctcc gcgacggtga catagccggt cgccgcgacg aacttcgcga
     3481 attggcggtt ggtgacctcg tgcggatcga tccagaaacc ggcgaccgtc gtcttccgca
     3541 ccggcccctc ttcggcatag acgtcatcct cgcccatctc gaaggtgccg cccgccagtt
     3601 tcaccggcgt atcggcgacg cccgcgcagg tgcgcgcaac cttcgcgccg gccgcgggcc
     3661 tgtccgtttc ggcggcatcg tcgccgcagg ccgaaagcgt aagcagcaag acgcccgcaa
     3721 cgaaggctag cgagggcatt cggatcatat tgggtctcca ctcgggaaaa tcttgataga
     3781 tatatcgccg aaacagaggc tgaacggccg agcggcgccg gtcaatgcac ggcgaccggc
     3841 gcctcgcctg cacggcgcga ggtcggcggg tcgatcacct gttgttcgga cacctcgacg
     3901 cgctcgagcg gcagcttggc gccgagcagc ggtccgaatt ttaagcggcg atcgggatgg
     3961 ccccggtcaa tatggcggcc gaggttagaa ccaggcttat cccaacggca tatttaagcg
     4021 cgaagcgctg ggcatcggcg acgtcgacgc ccagcaatcc gcaggccaga tagacgggtg
     4081 cgagcagtgg gctgagcgaa tggaccgggc cgcccatcag cgaggcgcgc gcgatcgctt
     4141 cggcgggaat gccataggtc tgaccggctt gggccaggac cggcacgacg ccgaaataat
     4201 aggcgtcgtt cgacatgacg aaagtaagcg gcagcgcgag caacgacgtg atcggcgcga
     4261 gataggggcc gaagctctcg ggcacgcccg ccagcagcga gcgcgccatc gcgtcggtca
     4321 ttcccgtgcc acccatgatg ccggtgaagg cgccggccgc gaagatcagc acgaccacca
     4381 tgaccacatt gtcggcgtgc gccgcgatcc gttcgcgctg atcggatagc cgcggatagt
     4441 tgaccgtcag cgctaccgcg agcgcgccca tgatcagcac cggcaacggc gcgagcccgc
     4501 tgaccattcc ggccatcaaa acggcggtca acagcaggtt gaaccagaac aggcgcggac
     4561 gccgctcggg ccgatccgcc gcgaccggcg g
//
LOCUS       DATMSU010000427         4298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_82831, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000427 DATMSU010000000
VERSION     DATMSU010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82831"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VN034_11455"
     CDS             complement(<1..220)
                     /locus_tag="VN034_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWV61265.1"
                     /translation="MADAPQIPDEAETRARWGAFAWTAENAAKAKAVIARYPAGRQRS
                     AVMPLLDLAQRQVGAETQTQGWLPVPVIE"
     gene            complement(213..458)
                     /locus_tag="VN034_11460"
     CDS             complement(213..458)
                     /locus_tag="VN034_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61266.1"
                     /translation="MTARVISWIDAGYREGAKRSNSVMGLLLAAIGLVALGVDNAAVR
                     VFAIALAVLVFALFGWRMLVVFRGMAIAEDRRKKTNG"
     gene            complement(455..1672)
                     /locus_tag="VN034_11465"
     CDS             complement(455..1672)
                     /locus_tag="VN034_11465"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWV61267.1"
                     /translation="MFEGYPVDTADTAGDQVVTNYTINFGPQHPAAHGVLRMVMELDG
                     EIIERVDPHVGLLHRGTEKLIEYKTYLQALPYFDRLDYCSPLGMEHSYVLAIEKLLDL
                     EVPARAQYLRVLFAELTRICNHMLNIGSHVMDVGAMTPNLWVFELREDCLNFFERASG
                     ARMHSAYFRPGGVHQDVPERLLYDIGEWCEKRLPKLFGDAMSLVIDNRIFKQRNVDIA
                     TVSKEDALAWGFSGPLIRGSGIAWDLRKSQPYDAYAAMDFDIPVGTRGDCYDRFMVRV
                     QEVYQSARIILQCLRDMPTGPIASLDRKVVPPKRGEMKQSMESLIHHFKLYTEGFHVP
                     AGEVYVATESPKGEFGVYLVSDGTNKPYRCKIRPTAFSHLQAMDMMSKGHMLADTTAI
                     IGAIDVVFGECDR"
     gene            complement(1708..2616)
                     /locus_tag="VN034_11470"
     CDS             complement(1708..2616)
                     /locus_tag="VN034_11470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWV61268.1"
                     /translation="MAHPAPLVAPQPSLAASLKTLLGADLVAHIFTVDEDSITVTREA
                     VAGVMVALRDNLEYQQLMEIAGVDYPDRPERFEVVYHLLSVTKNHRLRVRVSTDEATP
                     VPTIVPVWPNAGWLEREVFDMYGVLFAGNPDLRRILTDYGFQGHPQRKDFPLTGYTEL
                     RYSEEDKRVVYEPVRLAQDFRNFDFLSPWEGADYVLPGDEKAGGTGEAPGKGAPTPMT
                     AAEVKKADEEAEKEKASPPPTPKTTESTTQTGAGAKTNIKAAKTSRDGAKATTAAKAK
                     TPATKSAEKAPAKPRAPRKPKTGGDA"
     gene            complement(2616..3149)
                     /locus_tag="VN034_11475"
     CDS             complement(2616..3149)
                     /locus_tag="VN034_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWV61269.1"
                     /translation="MPGQMPVAPGTNPDQGFFDDLNSEVGDKGFLVTSTEDLFNWART
                     GSLWWMTFGLACCAVEMIHVNMPRYDMERFGAAPRASPRQSDVMIVAGTLCNKMAPAL
                     RRVYDQMSNPKYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLY
                     GVMQLQRKIRRVGTIER"
     gene            complement(3167..3541)
                     /locus_tag="VN034_11480"
     CDS             complement(3167..3541)
                     /locus_tag="VN034_11480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWV61270.1"
                     /translation="MVDLTQYLPILIFLVIAVGLSSAFVFLPMGVARLTGAHKPDPAK
                     LTEYECGFPAFEDARSQFDVRFYLVAILFIIFDLEAAFLFPWAVSLEEIGWAGWATMM
                     VFLAELTLGLVYAWKKGALDWE"
     gene            3764..4246
                     /locus_tag="VN034_11485"
     CDS             3764..4246
                     /locus_tag="VN034_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HWV61271.1"
                     /translation="MGPITARVALAFAPLALFPATPAHAELVNAANPETIKAIVESQG
                     WPATIVAKEGDDPYIESNRNGLKFLVLFMNCDGGERCKTLQYYMGFSDAKDVTLDRLN
                     QWNKEKRFARAYKDDAGDPVLEMDVDLDFQGIPRENVGETFNTWASLMDSFRAYVFEK
                     "
BASE COUNT          802 a         1357 c         1342 g          797 t
ORIGIN      
        1 attcgatcac cggcaccggc agccacccct gcgtctgcgt ctcggcgccg acctggcgct
       61 gcgcgaggtc gagcagcggc atcaccgccg agcgctgacg ccccgcgggg tagcgcgcga
      121 tgaccgcctt cgccttcgcg gcattttccg ccgtccacgc aaaagcgccc cagcgcgcgc
      181 gggtttcggc ctcgtcgggg atttggggtg cgtcagccat ttgtcttctt cctgcgatct
      241 tcggcgatcg ccattccccg gaagacgaca agcatgcgcc atccgaacaa agcaaatacc
      301 aaaaccgcca gagctattgc aaaaacacgt acagcagcgt tgtcgacgcc aagcgcgaca
      361 aggccaatag cggccaacag cagacccatc acagagttgg aacgcttggc tccctcgcga
      421 tagcctgcat caatccatga aatcactcgc gccgtcaccg atcacactcc ccgaacacca
      481 catcgatcgc gccgatgatc gcggtcgtat ccgcgagcat gtggcccttc gacatcatgt
      541 ccatcgcctg aaggtggctg aacgccgtcg ggcggatctt gcagcgatac ggtttgttgg
      601 tgccgtcgct gaccagatag acgccgaatt cgcccttggg gctttcggtc gccacataga
      661 cttcgcccgc cggaacgtgg aaaccttcgg tgtagagctt gaagtgatgg atcagcgatt
      721 ccatcgactg cttcatctcg ccgcgcttcg gcggcacgac cttgcggtcg aggctcgcga
      781 tcgggccggt gggcatgtcg cgcaggcact ggaggatgat gcgtgccgac tgatagactt
      841 cctgcacacg caccatgaag cggtcgtagc aatcgccgcg cgtgccgacg ggaatgtcga
      901 agtccatcgc ggcataggcg tcatagggct gcgacttgcg cagatcccac gcgatgccgc
      961 tgccgcggat cagcgggccc gagaagcccc atgccagcgc gtcttccttg ctgaccgtcg
     1021 cgatgtcgac gttgcgctgc ttgaagatgc ggttgtcgat gacgaggctc atcgcgtcgc
     1081 cgaacagctt cggcagccgc ttttcgcacc attcgccgat gtcgtagagc agccgctcgg
     1141 gcacatcctg atggacgccg ccgggacgga aataggccga gtgcatccgc gcgcccgacg
     1201 cgcgctcgaa gaagttcagg caatcctcgc gcagctcgaa cacccacagg ttcggggtca
     1261 tcgcgccgac gtccatgacg tgcgacccga tgttgagcat atgattgcag atgcgcgtca
     1321 gctcggcgaa cagcacccgc agatattgcg cgcgcgccgg cacctcgagg tcgagcaatt
     1381 tctcgatcgc gagcacatag ctgtgctcca tcccgagcgg cgagcaatag tcgagccggt
     1441 cgaaataggg cagcgcctgc agataggtct tatattcgat cagcttttcg gtgccgcggt
     1501 ggagcagccc gacgtgcgga tcgacgcgct cgatgatctc gccgtcgagc tccatcacca
     1561 tgcgcaaaac gccgtgcgcc gcggggtgct gcgggccgaa gttgatcgtg tagttggtga
     1621 cgacctgatc gcccgcggta tcggcggtat cgaccggata gccttcgaac atgtcgctgc
     1681 cgtggtgctt gacctgagga gagtcggtca tgcgtcacct cccgtcttgg gtttgcgcgg
     1741 agcgcgcggc ttggccggag ctttttccgc actcttggtc gccggcgttt tcgccttcgc
     1801 tgccgtcgtc gctttggcgc catcgcggct cgttttcgcg gccttgatat tcgttttcgc
     1861 gcccgcgccc gtctgcgtcg tgctttcggt cgtcttcggg gtcggcggcg gcgatgcctt
     1921 ttccttctcg gcttcttcgt cggccttctt gacctcggcc gccgtcatcg gcgtcggcgc
     1981 gcccttgccc ggcgcttcgc ccgtcccgcc ggccttttca tcgcccggca gcacatagtc
     2041 ggcgccttcc catgggctga ggaagtcgaa attgcggaaa tcctgcgcca gccgcacggg
     2101 ctcgtacacg acgcgcttgt cctcttcgga atagcgcagc tcggtgtagc cggtcagcgg
     2161 gaagtccttg cgctgcggat gcccctggaa accatagtcg gtcaggatgc ggcgcaggtc
     2221 gggattgccg gcgaacagca cgccgtacat gtcgaacact tcgcgctcga gccagccggc
     2281 gttcggccag acgggaacga tcgtcggcac cggcgtcgcc tcgtcggtcg acacgcggac
     2341 gcgcaggcgg tgattcttcg tcacgctgag cagatgatag acgacctcga accgctcggg
     2401 ccggtcggga tagtcgaccc cggcaatctc catcagctgc tgatattcga gattgtcgcg
     2461 cagcgcgacc atcaccccgg cgacggcttc gcgcgtcacg gtgatgctgt cttcatcgac
     2521 ggtgaatata tgcgcgacga ggtcggcgcc gagcagcgtc ttcagcgaag ccgcgagcga
     2581 aggctgcggc gcgacgaggg gagcgggatg ggccatcagc gttcgatcgt tccgacgcgg
     2641 cggatcttcc gctgcaactg catcacgcca tagagcaggg cttcggcggt cggcgggcag
     2701 ccgggaacat agatgtcgac gggcacgata cggtcgcagc cgcgcaccac cgaatagcta
     2761 taatgataat agccgccgcc attggcgcag ctgcccatcg agatgacata tttggggttc
     2821 gacatctggt cgtacacgcg gcgcagcgcg ggcgccatct tgttgcacag cgtccccgcg
     2881 acgatcatca cgtccgactg gcgcgggctc gcgcgcggcg cggcgccgaa gcgctccatg
     2941 tcgtaacgcg gcatgtttac gtggatcatc tcgaccgcgc agcaggcaag gccaaaggtc
     3001 atccaccaca gcgagccggt gcgcgcccag ttgaacagat cctcggtcga ggtgacgaga
     3061 aagcccttgt cgccgacctc tgaattgagg tcgtcgaaaa agccctgatc ggggttcgta
     3121 cccggggcga ccggcatctg gccgggcatg atgatgctgg aagtgctcat tcccaatcca
     3181 acgcgccttt tttccacgcg taaacaaggc cgagggtcag ttcggcaagg aagaccatca
     3241 tcgtcgccca accggcccag ccgatctcct cgaggctcac cgcccaggga aagaggaagg
     3301 cggcttcgag gtcgaagatg atgaaaagga tggcgacgag gtagaaacgc acgtcgaact
     3361 ggctgcgcgc gtcttcaaaa gcgggaaaac cgcattcata ttcggtcagc ttcgccggat
     3421 cgggcttgtg cgccccggtc agccgcgcga cgcccatggg caggaacacg aaggcggagg
     3481 acaggccgac ggcgatgacc agaaagatca gaatcggcaa atattgcgtc agatcgacca
     3541 tgggagctcg cagttctgtt gttttggggt gtcgcgcacc cgtttatggg gggcgcttta
     3601 ggccacgaag ccgcgtgtcg caagcactgc gggcaggatt ttcgttccct gcgtaaccaa
     3661 aaacatattg cgcgctggcg agcgggccta tcgctgtgat acgccatcga aaaagggccg
     3721 ctgcactgca gtttcagggc caaaatcgac aaagggagct ttcatgggac caatcaccgc
     3781 tcgcgtcgcc ctcgccttcg ccccgctggc gcttttcccg gcgactccgg cgcatgccga
     3841 actggtcaac gcggcgaatc cggaaacgat caaggcgatc gtcgagtcgc agggctggcc
     3901 cgcgacgatc gtcgccaagg agggcgacga tccctatatc gagagcaacc ggaacggcct
     3961 caaattcctc gtgctcttca tgaactgcga cgggggagag cggtgcaaga cactgcaata
     4021 ttatatgggc ttcagcgatg cgaaggacgt cacgctcgac cggctcaacc agtggaacaa
     4081 ggaaaagcgc ttcgcgcgcg cctataagga cgacgcgggc gacccggtgc tcgaaatgga
     4141 tgtcgacctc gatttccagg gcatcccgcg cgagaatgtt ggcgagacct tcaacacctg
     4201 ggcgtcgctg atggacagct tccgggccta tgttttcgag aaatgacctg aacaatcatt
     4261 cttcaccccg gacttgatcc ggggtccata accgcgcc
//
LOCUS       DATMSU010000428         6806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_121426,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000428 DATMSU010000000
VERSION     DATMSU010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6806
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121426"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VN034_11490"
     CDS             complement(<1..822)
                     /locus_tag="VN034_11490"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HWV61272.1"
                     /translation="MLRSIMRGFWLLLAAMGLSLTPAAAKDAKDKPYEHYVFGQLDTP
                     TPGPVSGGLLLMGGGDRNVDSMKWFFGKAGNGHIVVISASYGKEIGKEFFEEIGGIQS
                     AEIFVFHARSQATNRKILERLKKADGIFIAGGDQARYVRYWRGTPVGDILNAHVAAGK
                     PLAGTSAGLAIQGEKLYGAMDDGSITSPEALADPFGPANTIEGDFLHFALLKGIVTDT
                     HFKERERLGRLLAFVAKAQAGRPADQPAMLGLGVDESAAVAVEPDGSARIYATEPD"
     gene            1060..3798
                     /locus_tag="VN034_11495"
     CDS             1060..3798
                     /locus_tag="VN034_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61273.1"
                     /translation="MPALAAPALAQEASESGEQEIIVTGSRIPTIRDQGPAPVTTIDS
                     DTIRANGYTSVPELLAAMTQNSGETQSPQSGSSADFTPGAQQVDLRGLGPNHTLVLIN
                     GRRIADFPLPYIGRSNFTDISNIPVSLIDKVEILSGAGSAIYGSDAIAGVVNFKMKEK
                     LDGITLDYRHGRTQHGGGLSHRFTATGGWSTDRFNIAGGIEYLDQRPLWAYQRKIQDS
                     TDDEPGGGIPRRTFLRRNMEDEVYIDPGADTCEALSYLNGGSTIYASRPRWGYFDDAL
                     DDYGPGYFCGSKTSIGYGTIISGRKGFNSFGTMSYELSDNAKLFTDFQFGISKVRLMY
                     DVTSWSFEGSSSSSNNSFYNINPAVDSLDEWTRQFTPEETGGLEMQRSKQTTYSITPG
                     IRGNFGNAWNYELSFNYSRYKSIVRWPQIIASKANDLYLGEQLGVDEDGNPIFDADPT
                     RFYTPLTRAEYESISADTVYKPVSWTSNLQATITNGELFQLPAGPVGFAAVAEYGKQG
                     YDLNPDPLALTDYYYSWKDSDGKGKRSHAAVGGEFRVPVLDFVTVTGAGRYDHFRFGG
                     GGTGKFTYNGGLEVRPTESLLFRAAYGTAFRAPDLHYVFTGPGNVESSVDDYYLCRSD
                     PDEDYEDIGDCSYSGTGIVVNRNGNRDLKSETGRTLTAGAVFSPSRNLHISVDYFRVT
                     LDNQVDDLSVNQIARDEADCRLGETQAGTAVDINSPTCQDALARVTRFTTGPSIGEIA
                     SIRVNPINIAREKTSGIDVAFRGTLPTSFGDFTLSLAHSHVFKHSFQQYPGDPLIDKL
                     AADSGYYLPRDKSNGSITWASDGAQFTFSGTRLGKLPNYDEDAFIKASYLFNATLQYD
                     FTDHMRGSLTVRNVFDKNPVKDPTWTGYPYYNTSWFDSVGRSVFMQLTYKLGGSPL"
     gene            complement(3876..6719)
                     /locus_tag="VN034_11500"
     CDS             complement(3876..6719)
                     /locus_tag="VN034_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61274.1"
                     /translation="MTAQKRAEAISDVPIAISAFSAETLQEQKIESGAELVRGIPNVT
                     FSKNNYSGYNFSIRGVGTKAVSATSDPGVAVSFNNVALIRNRLFEQEYFDIERVEVLR
                     GPQGTLYGRNATAGVVNMITAKPDLDDFTGSGKVEVGNYKAMRATGMINIPLVDDILA
                     VRAAGAWTSRDGYGYNLATNDRIDGRDLWSTRLSLAFKPSPRLRINATWERFNENDDR
                     LRTGKQLCHRDPGPTTFPGVFDPDQPGQLRTLDPLESGRLSQGCRQGSLYDEGAYQSP
                     NGLSIPFIAAGQMLGLLGYDQPSVFPDGSWNNDAKFVRFLQYGVDPYNVEQSRDLRTI
                     FSHQPAEYRAEADIFALDVAFDVSDSLTVTSQTLYNEDRVYSLQDYHRFALVPTFNDA
                     RGLYTYYQCDWINNPATCSNETEFASFLPGGIFTDPQIGPSNAVAGIEVSSSESWQFS
                     QELRLTSDFDGPLNFSAGANYMKFKGLNDYYLFFNIVTMLAQGYYNESPNIGECLAFP
                     GNCMTVDPNPIGDLNGDGHNYFRNRNPYELESIAGFGEIYWDATPNLKVTAGLRYTED
                     RKKFTPWRSQLLVPGDSYGPSDPIRQKWGEFTGRFGIDWRPDLSFADDTMLYAFYSRG
                     YKGGGANPPPAQPIADYLVVSEIPPTFEPEFVNAFEVGFKNSFLGGAFTLNGSAFYYD
                     YTDYQVSKVVDRTIVNENFDASVWGAELEAVWNPVSRLRLNATAGYLDTRIKKGERSI
                     DIFNRTQGRDGWSVVSPWVQQTSNCILPNDYLTQIVNFGLFPAPATFASAIIQACNTW
                     TGVDALSHLGLSFDPADYPDANNGAGFYADLSGNELPNSPHMTFNIGAQYSVPIDSWE
                     VTLRGDYYRQSKSYSRVYNLESDRLRGWDNVNLSLTIARPEDDLVFQAYVKNLFNKTP
                     ITDAFLNSDSTGMTTNIFTLDPRIVGFSISKSF"
BASE COUNT         1373 a         2094 c         2053 g         1286 t
ORIGIN      
        1 gtcgggctcg gtcgcataga tgcgcgcgct gccgtcgggc tcgaccgcga ccgccgcgct
       61 ctcgtcgacg ccgaggccga gcatcgcggg ctgatctgcc gggcgcccgg cctgtgcctt
      121 ggcgacgaag gccaagagac ggccgagccg ctcgcgttcc ttgaaatgcg tgtcggtgac
      181 gatgcccttc agcagcgcga aatgcaggaa gtcgccttcg atcgtgttcg cggggccgaa
      241 gggatcggcg agcgcctcgg ggctcgtgat actgccatcg tccatcgcgc cgtagagctt
      301 ctcgccctgg atcgcgaggc ccgcgctggt gcccgcgagc ggtttgcccg ccgcgacatg
      361 cgcgttcagg atgtcgccga ccggcgtgcc gcgccaatag cggacatagc gcgcctgatc
      421 gccgcccgcg atgaagatgc cgtcggcctt cttcagccgc tcgagaatct ttctgttcgt
      481 cgcctgcgac cgcgcatgaa agacgaagat ttcggccgac tggatgccgc cgatctcctc
      541 gaagaattcc ttgccgattt ccttcccata ggatgcgctg atcacgacga tatgaccgtt
      601 ccccgccttg ccgaagaacc atttcatcga atcgacgtta cggtcgccgc cgcccatcag
      661 caggagcccg cccgataccg ggccgggggt cggcgtgtcg agctggccga acacataatg
      721 ttcgtaaggc ttgtccttcg cgtcttttgc agcggccggc gtaagcgaca gacccatcgc
      781 agcaagcaac agccaaaatc ctcgcatgat cgagcgcagc atccgtcttc cttccgacga
      841 ccccaatggt ccaacattac ataatgaccg catcaaaaga caatatgatt tccgaaatca
      901 tcatattgat tgaaattatc gacgttttct ggtcgagagg atgaaatatc cggtggggaa
      961 gcgattgggg tcgagtcgca ccgggaagga tgagggggct ggtcgatgac gaatttcgga
     1021 aggcgggcgt ttcgggttgt cctcgggctg ggcgtttgca tgcccgcgct ggccgcgccg
     1081 gcgctcgcgc aggaggcgag cgagagcggc gaacaggaaa tcatcgttac cggatcgcgc
     1141 attccgacga tccgcgacca agggcccgcg ccggtcacga cgatcgattc ggacacgatc
     1201 cgcgccaacg gctataccag cgtccccgaa ctgctcgccg cgatgacgca gaacagcggc
     1261 gagacgcaaa gcccgcagtc gggcagcagc gccgatttca ctccgggtgc gcaacaggtc
     1321 gatctgcgcg gccttggccc caaccacacg ctcgtcctga tcaacggccg ccgcatcgcc
     1381 gacttcccgc tgccctatat cgggcgcagc aacttcaccg atatctcgaa cattccggtc
     1441 agcctgatcg acaaggtcga aatcctgagc ggcgcgggct cggccatcta tggatcggac
     1501 gcgatcgcgg gcgtcgtcaa cttcaagatg aaggaaaagc tcgacggcat cacgctcgat
     1561 tatcgccacg gccgtaccca gcatggcggc gggctctcgc accgcttcac cgcgacgggt
     1621 ggctggtcga ccgatcgttt caatatcgcg ggcgggatcg aatatctcga tcagcgcccg
     1681 ctctgggcct atcagcgcaa gatccaggac agcaccgacg acgagcccgg cggcggcatc
     1741 ccgcggcgca ccttcctgcg ccgtaacatg gaggacgaag tctatatcga ccccggtgcc
     1801 gacacctgcg aggcgctctc atacctcaac ggcggctcga ccatctatgc ctcgcggccg
     1861 cgctggggct atttcgatga cgccctcgac gattatggtc cggggtattt ctgcggcagc
     1921 aagacatcga tcggctacgg cacgatcatt tcggggcgca agggcttcaa cagcttcggg
     1981 accatgtcgt acgagctgtc ggacaacgcc aagctgttca ccgacttcca gttcgggatc
     2041 agcaaggtcc gcctgatgta cgacgtcacg agctggagct ttgaaggctc gtcgagcagc
     2101 tcgaacaaca gcttctacaa catcaatccc gcggtcgact cgctcgacga atggacgcgc
     2161 cagttcacgc ccgaggaaac cggcgggctg gaaatgcagc gcagcaagca gacgacctac
     2221 agcatcacgc ccggtatccg cggaaatttc gggaacgcct ggaattacga gctgtcgttc
     2281 aactatagcc gctacaagtc gatcgtgcgc tggccgcaga ttatcgcgag taaggcgaac
     2341 gacctctatc tcggcgaaca gctcggcgtc gacgaggatg gcaatccgat cttcgatgcc
     2401 gatccgacgc gtttctacac cccgctgacg cgcgccgaat atgaaagcat ctcggccgac
     2461 acggtctaca agcccgtgag ctggacgagc aatttgcagg cgacgattac caatggcgag
     2521 ctgttccagc tgcccgcggg cccggtcggt ttcgccgcgg tcgccgaata tggcaagcag
     2581 ggctacgacc tcaatcccga tccgctcgcg ctcaccgact attattacag ctggaaagac
     2641 agcgacggca aaggcaagcg gagccatgcc gcggtcggcg gcgagtttcg cgtgcccgtg
     2701 ctcgatttcg tgacggtcac cggcgccggg cgctatgatc atttccgctt cggcggcggc
     2761 ggcacgggca agttcaccta taatggcggg ctcgaagtgc gaccgaccga aagcctgctt
     2821 ttccgcgcgg cctatggcac cgccttccgc gcgcccgacc tccattatgt ctttaccggc
     2881 ccgggcaatg tcgaaagcag cgtcgacgat tattatctct gccgttcgga tcccgacgag
     2941 gattatgagg acatcggcga ttgcagctat tcgggcacgg gcatcgtcgt gaaccgcaac
     3001 ggcaaccgcg acctcaaatc cgaaaccggg cggacactga ccgccggcgc cgttttctcg
     3061 ccgtcgcgca acctgcatat ctcggtcgat tatttccgtg tcacgctcga caaccaggtc
     3121 gacgacctca gcgtcaacca gatcgcgcgc gacgaggccg attgccggct tggagagacg
     3181 caggcgggaa ccgcggtcga tatcaattcg ccgacctgcc aggatgcgct cgcgcgcgtc
     3241 acccgcttca ccaccggacc gtcgatcggc gaaatcgcgt cgatccgcgt caacccgatc
     3301 aacatcgcac gcgaaaagac cagcgggatc gacgtcgcct tccgcggcac gctgccgacc
     3361 agcttcggcg acttcacgct gtcgctcgcg cacagtcatg tgttcaagca cagcttccag
     3421 caatatccgg gcgatccgct gatcgacaag ctggcggcgg acagtggcta ttatctgccg
     3481 cgcgacaaat cgaacggcag catcacctgg gcgtcggacg gcgcgcagtt caccttctcg
     3541 ggaacccgcc tcggcaagct gccgaattat gacgaggatg ctttcatcaa ggcgagctac
     3601 ctgttcaacg cgacgctgca atatgatttc accgatcata tgcgcgggtc gctgacggta
     3661 cgaaacgtct tcgacaagaa cccggtgaag gatccgacct ggacaggtta tccttattac
     3721 aacaccagct ggttcgacag cgtcggacgc agcgtgttta tgcagctgac ctataaactc
     3781 ggaggctccc cgctctgagc ttccccagcc ggaaaaagcg ggccggtgtc cgcgccttgt
     3841 gagcgcgaca tcggcccagt ttctctcgga gaggattaaa agctcttgct gatagaaaag
     3901 ccgacgatgc gcggatcgag cgtgaatata ttggtcgtca tccccgtcga atcggagttc
     3961 aggaacgcgt cggtgatcgg cgtcttgttg aagagattct tcacataggc ctgaaagacc
     4021 agatcgtcct cgggccgggc gatcgtcagc gacaggttga cgttatccca cccccgaagc
     4081 cggtcggact cgaggttata tacgcgcgaa tagcttttcg actggcgata ataatctccg
     4141 cgcagcgtca cttcccagct gtcgatgggc accgaatatt gcgcgccgat gttgaaggtc
     4201 atgtgcggcg agttgggaag ctcgttgccg gataggtcgg catagaaacc ggccccgtta
     4261 ttcgcgtcgg ggtagtcggc ggggtcgaaa gaaaggccca gatggctcag tgcgtcgacc
     4321 ccggtccagg tgttgcaagc ctggatgatc gccgacgcga aggttgcggg agccggaaaa
     4381 agcccgaaat tgacgatctg tgtcagatag tcgttgggca atatgcagtt cgacgtttgc
     4441 tgcacccacg gcgatacgac gctccaaccg tcgcggccct gcgtgcggtt gaagatatcg
     4501 atcgaccgct cgcccttctt gatccgggtg tcgagatagc ccgccgtcgc gttgaggcgc
     4561 aggcgggaaa ccgggttcca aacggcctcc agttccgccc cccagaccga cgcatcgaaa
     4621 ttctcgttga cgatggtgcg gtcgaccacc ttcgaaacct gatagtcggt ataatcataa
     4681 tagaaggcgg aaccgttcag cgtgaaggcg ccgccgagga agctgttctt gaagccgact
     4741 tcgaacgcat tgacgaattc gggctcgaac gtcggcggaa tctccgaaac gacgagataa
     4801 tcggcgatcg gctgcgcggg gggcggattg gcgccgccgc ctttgtagcc gcgcgaatag
     4861 aaggcataga gcatcgtatc gtcggcgaag gacagatcgg gtctccagtc gatcccgaaa
     4921 cgcccggtaa actcacccca tttctgcctg atcggatcgg acggcccata gctgtcgccg
     4981 ggcacgagca actggctccg ccacggcgtg aacttcttac ggtcttcggt gtaacgcagg
     5041 cccgccgtca ccttcagatt gggcgtggcg tcccaataga tttcgccgaa accggcgatc
     5101 gattcgagtt cgtaaggatt gcgattgcga aaataattgt gcccgtcgcc gttcagatcg
     5161 ccgatcgggt tgggatcgac ggtcatgcaa ttgccgggga aagcgagaca ttcgccgata
     5221 ttcggactct cgttgtaata gccttgcgcc agcatcgtca cgatattgaa gaacagataa
     5281 taatcgttga gccccttgaa cttcatatag ttggccccgg cgctgaaatt gagggggccg
     5341 tcgaagtccg aggtcagccg aagctcttgt gagaattgcc agctttcgct cgaagagact
     5401 tcgatccccg ccacggcgtt cgacggcccg atctgggggt cggtgaagat gccgccgggc
     5461 agaaaactgg cgaactcggt ctcgttgctg cacgtcgccg gattattgat ccagtcgcac
     5521 tgataatagg tgtaaagacc gcgtgcgtcg ttgaacgtcg gcaccagcgc gaagcggtgg
     5581 taatcctgca gcgaatagac ccggtcctca ttatagaggg tctgcgaggt aacagtcagt
     5641 gaatccgaga catcgaaagc gacatcgagc gcaaaaatat ccgcctccgc ccgatattcg
     5701 gccggctgat gcgaaaagat cgtccgcaga tcgcgtgact gttctacatt ataggggtcg
     5761 acgccatatt gcaggaagcg gacgaatttc gcatcattgt tccagcttcc gtccgggaac
     5821 accgatggct gatcgtaacc gagaaggccg agcatctgcc ccgccgcgat gaacggaatg
     5881 ctaaggccgt tcggcgactg ataggcgccc tcgtcataga gcgaaccttg ccggcaaccc
     5941 tgactgagac ggccgctttc gagcgggtcg agcgtgcgta gctggcccgg ctggtctgga
     6001 tcgaacacgc ccgggaaggt cgtcggcccc gggtcgcgat ggcagagttg cttaccggtg
     6061 cgcaggcggt cgtcattttc gttgaaacgc tcccacgtcg cattgatccg aagacgcggc
     6121 gaaggcttga acgccaggga aaggcgcgtc gaccaaagat cgcggccatc gatacgatcg
     6181 ttggtcgcca gattataacc atagccgtcg cggctggtcc acgcgcccgc ggcgcgaacc
     6241 gcgagtatat cgtcgacgag cggaatgttg atcatgcccg tcgcgcgcat cgccttgtaa
     6301 ttgccgacct cgacctttcc cgatccggta aagtcgtcga gatcgggctt cgcggttatc
     6361 atgttgacga cgccggccgt ggcgttccgt ccatagagcg tgccttgcgg accgcgcagc
     6421 acctcgacgc gctcgatgtc gaaatattcc tgctcgaaca aacgattgcg gataagcgcc
     6481 acattgttga aactgaccgc gacgccaggg tccgacgtcg cggacacggc cttcgtcccg
     6541 acgccgcgga tcgagaaatt gtagcccgag taattattct tcgaaaaggt cacattgggg
     6601 atgccgcgca ccagttcggc cccgctctcg atcttctgct cctgaagcgt ctcggccgag
     6661 aaagcactga tcgcgatcgg cacgtccgag atcgcctcgg cgcgtttttg cgcagtcacg
     6721 atgatctccg ccggcgcgct ggcttccgtg gcgaccgccc ggccgtcgga ggcgcccccc
     6781 gggttcgccg agacctgcgc ggccag
//
LOCUS       DATMSU010000429         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_64969, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000429 DATMSU010000000
VERSION     DATMSU010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64969"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /gene="trpS"
                     /locus_tag="VN034_11505"
     CDS             complement(<1..947)
                     /gene="trpS"
                     /locus_tag="VN034_11505"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWV61275.1"
                     /translation="MRTLSGIQPTGNLHLGNYLGAIRNWVRMQDEMAGDKLYFLADLH
                     AISMPHVPAELTANTRAMTAALVACGIDTDRSVLFNQARVPAHAELQWLLNGTARLGW
                     LNRMTQFKDKSGKNRESASAALYTYPVLQAADVLLYQTTHVPVGEDQKQHLELARDVA
                     QKFNNDYASEDAPVFTLPEPIIPAGLARIMSLRDGAAKMSKSDPSDMSRINLTDDADT
                     MMQKVRKAKTDPEVLPSEAAGLEGRPEARNLVGIYAALADTSVDAVLRDHAGQGFGAF
                     KPALGELLIEKLAPINARFVALKDDAAALDAILDKGAEKA"
     gene            complement(970..2547)
                     /gene="murJ"
                     /locus_tag="VN034_11510"
     CDS             complement(970..2547)
                     /gene="murJ"
                     /locus_tag="VN034_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWV61276.1"
                     /translation="MSSLVKSVGTIGGLTMVSRIFGFARDMLLSRILGAGGVADAWQL
                     AFQLPNIFRRLFAEGAFAAAFIPLFNQRMTKEGDHSKARAFADAVLAVLIPILIVFSA
                     LLLIVMPWVMGLFASDALQADGKTFDLAVLMARIAFPYLALMSVATLFAAILNSLSRF
                     AAAAAAPILLNICLIGALLLGLFTGDGGEASKAATGLYLAISVSLSGLFQLGWLYYWI
                     RRSGFRPALRRPRLTADVREMGVLILPAVFGAGVYQISRFIDLFFIATLPDKSITYLA
                     MADRLNQLPLGIIGIALGTAILPALSRFVAQDDRDGASRLQSNAVELAMLLTVPAAVA
                     LFVAGPAITSAFYVGGQYSVADGLATGAVVGGLVVGLPAYVLVKVLVPNFFARKDTRT
                     PVWTAAISLAINIALNLLLIPRLGIVGLALAGSIAAWCNVAMLYTILHRKGLFRLSGR
                     VAGRIVRIVLAAAAMAAALYYAIPLAGDAFIGGVFERIVALGTLVATGAIIFFGCAFL
                     FGVVNRDTVGQLRRRQL"
     gene            complement(2575..3090)
                     /gene="secB"
                     /locus_tag="VN034_11515"
     CDS             complement(2575..3090)
                     /gene="secB"
                     /locus_tag="VN034_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HWV61277.1"
                     /translation="MADETSPDLNNPALQPNGEDTSPAIGLISQYVKDLSFENPNAPA
                     VYQWQEAPQVDVQFNIAADNVGENLYEVTLKIDVTSKTDAGTSFVIDLKYAGLFGVRN
                     VPDDQLQPFFLAEAPRILFPFARRVVADAVQDGGFPPLLLEPIDFHGLFAQQVQAAQS
                     EAAGEVAVGQA"
     gene            3373..>3510
                     /locus_tag="VN034_11520"
     CDS             3373..>3510
                     /locus_tag="VN034_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HWV61278.1"
                     /translation="MIAAFLGMRLYSVLGKRTGHEQEPVLPRERTAASPVRLDENDGS
                     QA"
BASE COUNT          611 a         1102 c         1222 g          575 t
ORIGIN      
        1 gccttttcgg cgcccttgtc gaggatcgcg tcgagcgccg cggcatcgtc cttcagcgcg
       61 acgaagcgcg cgttgatcgg cgcgagcttt tcgatcagca attcgccgag cgcgggcttg
      121 aacgccccga acccttgccc cgcatggtcg cgcagcaccg cgtcgacgct cgtgtcggcg
      181 agcgcggcat agatgccgac gaggttgcgc gcctcggggc gcccctcgag ccccgcggct
      241 tcggacggca gcacctcggg atcggtcttc gccttacgga ccttctgcat catcgtgtcg
      301 gcgtcgtcgg tcaggttgat gcggctcatg tccgacgggt cggacttcga cattttcgcg
      361 gcgccgtcgc gcaggctcat gatgcgcgcg aggcccgccg ggatgatcgg ttcgggcagc
      421 gtgaacaccg gcgcgtcttc gctggcataa tcattgttga acttctgggc gacgtcgcgc
      481 gcgagttcga gatgctgttt ctggtcctcg ccgacgggga cgtgcgtcgt ctgatagagc
      541 agcacgtcgg ccgcctgcag gacggggtag gtatagagcg ccgccgacgc gctttcgcgg
      601 ttcttgcccg acttgtcctt gaactgcgtc atgcggttga gccagccgag ccgcgcggtg
      661 ccgttcaaaa gccattgcag ctcggcgtgc gcgggaacgc gcgcctggtt gaacagcacc
      721 gaccggtcgg tgtcgatccc gcaggcgacg agcgcggcgg tcatcgcgcg ggtgttggcg
      781 gtcagctcgg cggggacgtg cggcatcgag atcgcgtgga gatcggcgag gaaatagagc
      841 ttgtcgcccg ccatctcgtc ctgcatccgc acccagttgc ggatcgcgcc cagataattg
      901 ccgaggtgca aatttccggt gggctggatg cccgatagcg tccgcatgtc gatcgtcccg
      961 tctgaagttt tagagttggc gccggcgcag ctggccgacg gtgtcgcgat tgaccacgcc
     1021 gaacaggaag gcgcaaccaa agaatatgat cgcccccgtc gccaccagcg tgccgagcgc
     1081 gacgatccgt tcgaacactc cgccgatgaa ggcgtcgccc gccagcggga tcgcataata
     1141 gagcgccgcc gccatcgccg ccgcggcgag gacgatgcgg acgattcgcc cggctacccg
     1201 gccggagagg cggaacagcc ccttgcggtg caggatcgtg tacagcatcg ccacattgca
     1261 ccacgccgcg atcgagcccg cgagcgcgag cccgacgatg ccgagccgcg ggatcagcag
     1321 gaggttgagc gcgatgttga tcgccagcga aatcgccgcg gtccacaccg gcgtgcgcgt
     1381 gtccttgcgc gcgaagaaat tggggacgag caccttgacc agcacatagg cgggcagccc
     1441 gacgacgagc ccgccgacga ccgcgccggt ggcgagcccg tcggcgacgc tatattgccc
     1501 cccgacatag aaggcgctgg tgatcgcggg ccccgcgacg aacaaggcga cggcggcggg
     1561 gacggtgagc agcatcgcca gttcgaccgc gttgctctgc aggcgcgagg cgccgtcgcg
     1621 atcgtcctgc gcgacgaagc gcgacagcgc gggcaggatc gcggtaccga gcgcgatgcc
     1681 gatgattccg agcggcagct ggttgagccg gtcggccatc gcgagatagg tgatgctctt
     1741 gtcgggcagc gtcgcgatga agaaaaggtc gatgaagcgg ctgatctgat agacgccggc
     1801 gccgaacacc gcgggcagga tcagcacccc catctcgcgc acgtccgcgg tcagccgggg
     1861 gcggcgcagc gccgggcgaa accccgaccg ccggatccag taatagagcc agccgagctg
     1921 gaacaggccc gaaagcgaga ccgagatcgc gagataaagc cccgtcgcgg ctttcgaggc
     1981 ctcgccgcca tcgccggtga acagtccgag caatagcgcg ccgatcaggc agatgttgag
     2041 caggatcggc gcggcggcgg cggcggcgaa gcgcgacagg ctgttgagga tcgccgcgaa
     2101 taacgtcgcg acgctcatca aagcgagata gggaaaggcg atgcgcgcca tcaggacggc
     2161 gaggtcgaag gttttgccgt cggcctgcag cgcgtcgctc gcgaacagcc ccatcaccca
     2221 cggcatcacg atcagcagca gcgccgaaaa gacgatcagg atcgggatca gcaccgcgag
     2281 caccgcgtcg gcaaaggcgc gcgccttgct gtgatcgccc tccttggtca tccgctggtt
     2341 gaacagcggg atgaaggcgg cggcgaacgc gccctcggcg aacaggcggc ggaagatgtt
     2401 gggcagctga aaggcgagct gccaggcgtc ggcgacaccg ccggcgccga gaatgcggct
     2461 gagcagcatg tcgcgcgcga agccgaaaat gcggctgacc atcgtcaggc cgccgatcgt
     2521 ccccacgctt ttgacgaggc tgctcaagcc ctcagcccgc ccccgttaca gggctcaggc
     2581 ctggccgacc gcgacttcgc cggccgcttc ggattgcgcc gcctggacct gctgcgcgaa
     2641 aaggccgtgg aaatcgatcg gttcgagcag cagaggcggg aaaccgccat cctgcaccgc
     2701 atcggcgacg acgcggcgcg cgaaggggaa gaggatgcgc ggcgcttcgg cgaggaagaa
     2761 gggctgaagc tggtcgtcgg ggacgttgcg gacgccgaac aggccggcgt atttgaggtc
     2821 gatcacgaag ctcgtgccgg cgtcggtttt cgacgtgacg tcgatcttca gcgtcacttc
     2881 gtacagattc tcgccgacat tgtcggcggc gatgttgaac tgcacatcga cctgcggcgc
     2941 ttcctgccac tggtaaacgg cgggggcgtt cgggttttcg aacgacaggt ccttcacata
     3001 ttgcgaaatc aggccgatcg ccgggctcgt gtcttcgccg ttcggttgca gggcgggatt
     3061 gttgaggtcg gggctcgtct cgtcggccat gatgaagctg ctttcactga aaagatggaa
     3121 acaatatccg catccgcacg ggccgtgtcg gaaccgcggc ggacggttgc cgcggcgctt
     3181 agcagcgccc ggaggtgagc acaatgggcc cgatggcggt cgatggttaa gggcgaatcg
     3241 ttgacccggt gcgtcggagc ggtcatggct atttgaaact aggcggcgta tcgcctatgt
     3301 atcggtagat atgtttctgc tttccacatt ggacttttgc ccgtgaccgc tttttcgatc
     3361 gtcttgctcg ccatgatcgc cgctttcctc gggatgcggc tctactcggt gctcggcaag
     3421 cgcacggggc atgagcagga gcccgtgctg ccgcgcgagc gcaccgccgc ctcgccggtc
     3481 cggctcgacg agaatgacgg gtcgcaggcg
//
LOCUS       DATMSU010000430         6893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_54777, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000430 DATMSU010000000
VERSION     DATMSU010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54777"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VN034_11525"
     CDS             <1..1246
                     /locus_tag="VN034_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWV61279.1"
                     /translation="NVRTPALQRLASEGMSFDRAFVASPACAPSRAALLTGMMPARNG
                     AEANHDRANAAIRKLPSYLQEQGYEVVAFGKVAHYQHTGTYGFDYYANDTFHDHKGIP
                     AAAEWLKARKDKRPLALFVGTNWPHVPWPRTTEGYDPAAMKLPPKTVDTPITRDARAR
                     FYAAVTRMDQDLASTMDAVDAVLGKDTFVLYSTDHGTQWPFGKWNLYDTGTRVPMVVR
                     WNGHVKAGTRTDAMVSWVDVLPTLVELAGGKKPEGIDGLSFANLFGVGQAAAGRKEIF
                     TTHNNDGRVNVYPMRSIRTDRWKYIQNLHPDWTYTTHIDLKVMRTDSGAYFPSWREAA
                     DKDPTAKAIVDSYYKRPAEELYDLKADPDEKVNLAGDPRYAKPLADLRGRLAAWRKSQ
                     GDSGSVAVKPRFERGAMSGEGD"
     gene            1437..4250
                     /locus_tag="VN034_11530"
     CDS             1437..4250
                     /locus_tag="VN034_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007679779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61280.1"
                     /translation="MRKTLLGAASATAVFVSMPAVAQDAPPPTAQNPAPQAENTDGDI
                     VVTGVRQSLERAAEVKRNATQVVDSIVATDIGKLPDPTVAAALQRVPGIQVQNDRNNE
                     LSTVRIRGLTDILTTVNGREVVTTTGRGFDLQDVPAEALARIDAFKSQTADQIEGGVA
                     GALDLRLNRPFSFRDPTLVLTARQNYATTADASNPQFGALAAAKTDSSIGEIGALVNV
                     TYSQAKNIRSVTNLGERRFVSGAPFGTADIMMPQVLRNMPDVGDIKRFQANAALQWQA
                     TPSLQFYADGLYTYFNSTTGFAGFNPQPFTNGSQISEVTLSDYCIDARVNPAGTNPQI
                     ITNVDPDTGKVTQTLQPVDVKTMCEPISARFDNVVVNQNSSSEEVTQRNKMVAGGLIF
                     EQDRGVLKVDVAYQTSRSVTENFNAEVGQRVPTVFVEFDDNNGPSFTIDPSIPLSSEN
                     VSLRNSINQNFSAGEGSLFQARLDSEYEFDGILTKLRGGLRYAKREALFQQVRQTNTV
                     QSIGFGNIGTPSEANARLISSLPLSPDFLGVIGVAPRLNGGQAFLGVNPAYLRSERGR
                     NELRDLFGLPLEAPAYDPTKEFNASEATYAAYMEGSYEIPIGALTLDGVVGTRVVKTD
                     RTISGFERVGDSDAFSPIVAKRSDVDILPSATARLKFPGSFQTRLTYSRSMRRPDFGS
                     LNPAESLTVVGNVFLINNGTRGNPDLRPQKSDSIDLTAEYYFDSGYVAVTGYYRSIKD
                     RVVTANRQEEIGGENYLISTPRNVGSVDLKGIEVSAQYFFDFLPGALSGFGMQGAFTL
                     ADSKVKGDDPLAGNPLVGVSKYNYTAGLLYDKKGLSGRLIWTFRSKYINGDNTGGVAV
                     RPYDESRPVNDPYLPVFLSYVRPAGRLDFSIGYDVTDALRLDIGGTNILRNKTSTYWG
                     DKRVVLALQGDETVYSIGARVKF"
     gene            4268..5350
                     /locus_tag="VN034_11535"
     CDS             4268..5350
                     /locus_tag="VN034_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HWV61281.1"
                     /translation="MNVACKKGTAGSIRRLDKPHDGRIRGSVALLAAALLAGMAAPAG
                     GAEAPPATFTNPLLTSGPDPWITQVDGVYYYTHTLGNRLMLWRTDNIADLAKAEKRVV
                     WTPPAKGLNAHSIWAPELHRLDGKWYLYYSATASSFKDDKHRAVFVLENSGADPMRGE
                     WIDRGRVNTAHAGIDGTVFEHGGKRYFAYSPYIGPDSGLAIAEMSDPWTLTGPESVIA
                     MPDKVWEKQGGRQILEGPEFLRGPKGDLFLTYSASACWSDDYALGLLHAPAGSDVLDP
                     KVWTKSAQPVFKSANGVYATGHNGFFTSPDGKESWIIYHANPGPGMKCTPARAPHIQR
                     FGWSEAGWPQFGEPVASDTPLPVPSR"
     gene            complement(5426..>6893)
                     /locus_tag="VN034_11540"
     CDS             complement(5426..>6893)
                     /locus_tag="VN034_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HWV61282.1"
                     /translation="APAPAPATAQRPNVLLILIDDLKPALGTYGDPTAITPNMDRLAA
                     RGVRFDLAYANQAVCAPSRINLMTGARSTSSGIYNFGMNLRDYMPGAVTLPQYFMRAG
                     YHTESMGKVYHIGHGTVGDTAGWSVPHHKEKVIEYVDPASTGGKPTQEEALFEEIPVP
                     EGNPFAYARTLARGAAWEMPDVADEAYADGRTARRAGERLKALKNGKQPFFLAVGFAR
                     PHLPFSVPKRYWDMYDPAKLPMPEFEQLPEGAPAYTDKQGGEINAYREPPRNVRGDQF
                     PPELKRQLLHGYYAGVSYVDVQIGKVLQALEREGLADNTVVVLWGDHGYHLGDHGIWT
                     KHTNMEQATHIPLIFAGPGIVKGRSTDQLAETVDIYPTLAALAALPAPNGPQPIDGTS
                     LAPVLHDPAARVRGYAYHAYPRPGRLGQAIRTERYRLVRWTQEKTGARDYELYDLIAD
                     PQETRNQANALPKVRAELDAILDAQPAAQPINGKPRRN"
BASE COUNT         1252 a         2185 c         2225 g         1231 t
ORIGIN      
        1 gaatgtccgg accccggcgc tgcagcgcct cgcgtccgaa ggcatgtcgt tcgaccgcgc
       61 tttcgtcgcc tcgccggcgt gcgcgccgag ccgcgccgcg ttgctgaccg gaatgatgcc
      121 cgcgcgcaac ggggcggagg cgaaccacga ccgcgccaac gccgcgatcc gcaagctgcc
      181 ctcctacctc caggaacagg gctatgaggt cgtcgccttc ggcaaggtcg cgcattacca
      241 gcacaccgga acctatgggt tcgactatta tgcgaacgat acctttcacg atcacaaggg
      301 catccccgcc gccgccgaat ggctgaaggc gcgcaaggac aagcgcccac tcgcgctgtt
      361 cgtcggcacg aactggccgc atgtgccgtg gccgcggacg accgagggct atgatcccgc
      421 cgcgatgaaa ttgccgccga agacggtcga tacgccgatc acccgcgacg cgcgcgcgcg
      481 cttctatgct gcggtcacgc ggatggatca ggatcttgcc tcgacgatgg acgcggtcga
      541 cgcggtgctg gggaaggaca cgttcgtcct ttattcgacc gaccatggca cccaatggcc
      601 gttcggcaag tggaacctct atgacacggg cacccgcgtg ccgatggtcg tgcgctggaa
      661 cggccatgtg aaggcgggaa cccgcaccga cgcgatggtc agctgggtcg atgtgctgcc
      721 gacgctggtc gagctggccg gcggcaagaa gcccgaaggt atcgacggcc tttcgttcgc
      781 gaacctcttc ggtgtcggac aggcggcggc gggccgcaag gaaatcttca cgacccacaa
      841 caacgacggc cgcgtcaacg tctatccgat gcgcagcatt cgcaccgacc gctggaaata
      901 tatccagaac ctgcatcccg actggaccta tacgacccac atcgacctga aggtcatgcg
      961 caccgattcg ggcgcctatt tcccctcgtg gcgcgaggcg gccgacaagg accccaccgc
     1021 caaggcgatc gtcgacagct attacaagcg tcccgccgag gagctctatg acctgaaggc
     1081 ggaccccgat gagaaggtga accttgcggg tgatccgcgt tatgccaagc cattggctga
     1141 tctccgcggc cgcctcgcag cctggcgcaa atcgcagggc gatagcggat cggtcgccgt
     1201 caaacctcgc ttcgaacgcg gggcgatgag cggcgagggc gattgattcg cggccacggt
     1261 tcgaacctga gagataatgc gccggggcct ggctccggcg ctttttttcg attgcgtgct
     1321 gcatgtcgcg atcgcgcgtt gatctattct ccgttttata ttatttatcc tctaagagtc
     1381 gccctggatc ggggttggtg acgagcgcgc gcaacgcgcc gaacagggag agggttatgc
     1441 gtaagacttt gttgggcgcc gcgtcggcga cggctgtgtt tgtgtcgatg ccggctgtcg
     1501 cgcaggatgc accgcctccg acagcgcaga atccggcccc gcaagcggaa aacaccgacg
     1561 gcgatatcgt cgtcaccggc gtacggcagt cgctcgaacg cgcggccgag gtgaagcgca
     1621 acgcgacgca ggtcgtcgat tcgatcgttg cgaccgacat cggcaaattg cccgacccga
     1681 ccgtcgccgc cgccttgcag cgcgtccccg gcatccaggt ccagaacgac cggaataacg
     1741 agctttcgac cgtccgcatc cgcggcctca ccgacatatt gacgacggtg aacgggcgcg
     1801 aggtggtgac cacgacgggg cgcggcttcg acttgcagga cgtgcccgcc gaagctctcg
     1861 cgcggatcga cgcgttcaaa tcgcagaccg ccgaccagat cgagggcggc gtcgccggcg
     1921 cgctcgacct gcggctcaac cgccccttca gcttccgcga tccgacgctg gtcctgaccg
     1981 cccgccagaa ttatgcgacg accgccgatg cgagcaaccc gcagttcggt gcgctcgcag
     2041 ccgccaagac cgattcgtcg atcggcgaga tcggtgcact ggtcaacgta acctattcgc
     2101 aggcgaagaa tattcgcagc gtgaccaatc tgggcgaacg ccgcttcgtt tcgggggcgc
     2161 cgttcggtac cgccgacatc atgatgccgc aggtgctgcg caacatgccc gacgtcggag
     2221 acatcaagcg ttttcaggcc aatgccgcgc tgcaatggca ggcgaccccg tcgctgcaat
     2281 tctatgccga cggcctctat acctatttca acagcaccac gggcttcgcc ggtttcaatc
     2341 cccagccgtt caccaatggt tcgcagattt cggaggtgac gctcagcgat tattgcatcg
     2401 acgcgcgcgt caatcccgcc gggaccaacc cgcagatcat caccaatgtc gatccggata
     2461 ccgggaaagt gacgcaaacg cttcagccgg tggacgtcaa aaccatgtgc gagcccataa
     2521 gcgcgcgctt cgacaatgtc gtggtcaatc agaacagctc gtccgaagag gtgacgcagc
     2581 ggaacaagat ggtcgccggc ggcctgattt tcgaacagga tcgcggcgtg ctcaaggtcg
     2641 acgtcgccta tcagacgtcg cgctcggtga ccgagaattt caacgcggaa gtcggccagc
     2701 gggtgccgac ggtcttcgta gaattcgacg acaacaacgg tccgtccttc acgatcgacc
     2761 cgtcgatccc gctcagctcc gaaaatgtct cgctgcgcaa ttcgatcaac cagaatttct
     2821 cggcgggcga gggcagcctg ttccaggcgc ggctggacag cgaatatgaa ttcgacggca
     2881 tcctgaccaa gctgcggggc ggcttgcgct atgccaagcg cgaggcgctg tttcagcaag
     2941 tgcgtcagac caacaccgtg cagtcgatcg gtttcggcaa tatcggcacg ccgtcggagg
     3001 cgaatgcgcg gctgatttcc tctttgccgc tatcgcccga tttcctgggc gtgatcggcg
     3061 tcgcgccgcg gctcaacggc gggcaggcgt ttctgggcgt gaaccccgcc tatctgcggt
     3121 ccgaacgcgg gcgtaacgag cttcgtgatc tgttcggcct gccgctggag gcgccggctt
     3181 acgatccgac caaggaattc aacgccagcg aggcgactta cgccgcctat atggaaggca
     3241 gctatgaaat cccgatcggt gcgctcaccc tcgacggggt ggtcggcacc cgcgtcgtca
     3301 agaccgaccg caccatttcg ggcttcgagc gggtcgggga cagcgacgcc ttcagcccga
     3361 tcgtcgccaa acggtcggac gtcgacattc tgccgtcggc gaccgcgcgc ctgaaattcc
     3421 ccggttcgtt ccagacgcgc ctgacctatt cgcggtcgat gcggcgcccc gatttcggct
     3481 cgctcaaccc ggcggaatcg ctcaccgtgg tcggcaatgt gttcctgatc aacaacggca
     3541 cgcgcggtaa tcccgacctg cgcccgcaga aatcggacag catcgacctc accgccgaat
     3601 attatttcga cagcggctat gtcgcggtca ccggctatta tcgctcgatc aaggaccgcg
     3661 tcgtgacggc aaaccggcag gaggagatcg ggggcgaaaa ttacctgatc tccaccccgc
     3721 gcaacgtcgg gtcggtcgac ctcaaaggca tcgaggtcag cgcgcaatat ttcttcgact
     3781 tcctgccggg cgcgctttcg ggcttcggca tgcagggcgc cttcacactg gcggattcga
     3841 aggtcaaggg cgacgatccg ctcgcgggta acccgctggt cggtgtctcc aaatataatt
     3901 acaccgccgg gctgctctat gacaaaaagg gcttgagcgg acgcctgatc tggaccttcc
     3961 gttccaaata tatcaacggc gacaataccg gcggcgtcgc ggtgcggccc tatgacgaga
     4021 gccggccggt caacgatcct tatctcccgg tgttcctgtc ctatgtccgt cccgccggcc
     4081 gtctcgactt cagtatcggc tatgacgtga ccgacgcgct gcggctcgac atcggcggca
     4141 ccaacatcct gcgcaacaag acgtcgacct attggggcga caagcgggtg gtgttggcac
     4201 tgcaaggcga cgaaacggtc tatagcatcg gcgcccgggt gaaattctga gctgacgagg
     4261 aaatcaaatg aatgtcgcct gcaaaaaggg gacggcgggt tcgatccgcc gcctcgacaa
     4321 gccgcacgac ggacgcatcc gggggtcggt cgcgctgctc gcggcggcgc ttctcgccgg
     4381 catggccgct ccggcgggcg gagcggaagc gccgccggcg actttcacca accccttgct
     4441 cacctcgggc ccagatccgt ggatcacgca ggtagacggc gtctattatt acacgcacac
     4501 gctgggcaac cgcctgatgc tctggcggac cgataatatc gccgatcttg ccaaagcgga
     4561 gaagcgcgtc gtgtggacgc cgcccgccaa ggggctgaac gcgcacagca tctgggcgcc
     4621 cgaactccac cgtctcgacg gcaaatggta tctctattat tcggcgaccg cgagcagctt
     4681 caaggacgac aagcaccgcg cggtgttcgt cctcgagaac agcggcgccg atccgatgcg
     4741 gggcgaatgg atcgacaggg gacgcgtgaa taccgcgcat gccgggatcg acggaaccgt
     4801 ctttgagcat ggcggaaaac gctatttcgc ttactcgccc tatatcggcc ccgatagcgg
     4861 gctggcgatc gcggaaatga gcgacccctg gacgctcacc gggcccgaaa gcgtgatcgc
     4921 gatgcccgac aaggtctggg aaaagcaggg cgggcgccag atcctcgaag gtcccgaatt
     4981 cttgcgaggg ccgaagggcg acctgttcct gacctattcg gcgagcgcct gctggtccga
     5041 tgattatgcg ctgggattgc tccacgcgcc cgcgggatcg gatgtgctcg atcccaaggt
     5101 ctggaccaag agtgcccagc cggtcttcaa gagcgcgaac ggcgtttatg cgaccggaca
     5161 caatggcttc tttacttcgc ccgacggcaa ggaaagctgg atcatctatc acgccaatcc
     5221 tgggccgggc atgaaatgca cgcccgcgcg agcgccgcat atccagcgtt tcgggtggag
     5281 cgaggcgggg tggccgcagt tcggcgagcc ggtcgccagc gacacgccgc ttccggtgcc
     5341 gtcgcgctga cgctgatctg gagttgtgat gaacaaagaa aggccgcgga cattcccgcg
     5401 gcctttcttc tttctccctg cttcctcagt tgcggcgggg cttgccgttg atcggctgcg
     5461 ccgcgggctg ggcgtcgagg atcgcgtcga gttcggcgcg caccttgggc aaggcattcg
     5521 cctggttgcg cgtctcctgg ggatcggcga tcagatcata gagttcatag tcgcgcgcgc
     5581 cggtcttttc ctgcgtccag cggacgaggc gataacgctc ggtgcggatc gcctgcccga
     5641 gccgccccgg gcgcggatag gcgtgatagg catagccgcg cacgcgcgcc gccggatcgt
     5701 gcaacaccgg cgccagcgag gtgccgtcga tcggctgcgg gccgttaggg gcgggcaggg
     5761 cggcgagcgc ggcgagtgtc ggatagatgt cgaccgtctc ggcgagttgg tcggtcgagc
     5821 gtcccttgac gatgccgggg ccggcgaaga tcagcgggat gtgcgtcgcc tgctccatat
     5881 tggtgtgctt ggtccagatg ccatgatcgc cgaggtgata gccatgatcg ccccacagga
     5941 cgacgacggt gttgtcggca agcccttcgc gttcgagcgc ctggagcacc ttgccgatct
     6001 ggacgtcgac ataggaaacg ccggcataat agccgtgcag aagctggcgt ttgagttcag
     6061 gcggaaactg gtcgccgcgc acatttcgcg gcggctcgcg ataggcgttg atctcgccgc
     6121 cctgcttgtc ggtataggcg ggcgcgcctt cggggagctg ttcgaattcg ggcatcggca
     6181 gctttgccgg gtcatacatg tcccaataac gcttcgggac cgaaaacggc aggtgcggac
     6241 gcgcgaagcc gacggcgagg aagaagggct gcttgccgtt tttcagcgcc ttcagccgtt
     6301 ccccggcgcg cctggcggtg cggccgtcgg cataggcttc gtccgcgaca tcgggcatct
     6361 cccacgcggc gccgcgcgcg agcgtgcggg cataggcgaa ggggttgcct tcgggcaccg
     6421 gaatttcctc gaacagcgct tcttcctgcg tcggtttccc gccggtgctc gcgggatcga
     6481 catattcgat caccttttcc ttgtggtgcg gaaccgacca gccggcggtg tcgccgacgg
     6541 tgccgtggcc gatgtgatag accttgccca tgctttcggt gtgatagccg gcgcgcatga
     6601 aatattgcgg cagcgtcacc gcgccgggca tataatcgcg caggttcatc ccgaaattat
     6661 agatgccgct gctcgtcgag cgcgcgccgg tcatcaggtt gatgcggctc ggcgcgcaga
     6721 cggcctgatt ggcataggcg agatcgaaac gcacgccgcg cgccgcgagg cgatccatgt
     6781 tcggggtgat cgccgtcggg tcgccatagg tgccgagcgc gggcttgaga tcgtcgatga
     6841 ggatgaggag gacgttgggg cgctgcgccg tggcgggggc cggtgcgggc gcc
//
LOCUS       DATMSU010000431         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_139941,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000431 DATMSU010000000
VERSION     DATMSU010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139941"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VN034_11545"
     CDS             complement(<1..906)
                     /locus_tag="VN034_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HWV61283.1"
                     /translation="MHELAFPLLVGLVILALAFDFLNGLHDAANSIATVVATRLLRPV
                     QAVLFAAFFNFAAYFLSIIFPELHKVAETIGKGIIDKDLVTPAVVFGALVGAMFWNVV
                     TWLKGIPSSSSHALVGGIVGAGVAHAGFEGIEWTGLNKTVIAIFLSPLLGMLLAMLVM
                     LVSSWALRRSTANFAEKTFRRLHLLSSAAYSISHGLNDAQKTMGIITVLLYSTGYLGG
                     EFHIPHWVAFSCYVAIALGTLSGGWKIIETMGGRITKLSHHQGFAASTGGSIMVFTAS
                     LLGIPVSTTHTITGSIIGAGVARRAS"
     gene            complement(922..2046)
                     /locus_tag="VN034_11550"
     CDS             complement(922..2046)
                     /locus_tag="VN034_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HWV61284.1"
                     /translation="MRQIAVLPYRFGGPAQDGPTEILLITSRETKRWVVPKGNPLNGM
                     TRHAAAAVEAEEEAGVIGAVCPTPIGSYEYRKRRANGASIMYNVEVFPLAVTNELADW
                     KEMDERDRKWFSFREAASAVEEPDLQALIRSFGDSGFRAVAQPRGVVENVADKTGVSW
                     MFAWFQRLLPRQGNFFELFESHAATLVAGANALSRMLQGGEGMADHVQEIIEREHDAD
                     AITREVLQTVRRTFLTPFDRSAITDLIASMDDAIDEMQKTAGAVDLYDVTEFEPEMRD
                     IAGIIVDAARLTAEALPLLRNISANAVRLHELTERLVRMEGHADEIHAAGLKRLFKEH
                     GQSNSVRFLIGRELYRHLERVTDSFEDVANEIDGLVIDHA"
     gene            2242..>3030
                     /locus_tag="VN034_11555"
     CDS             2242..>3030
                     /locus_tag="VN034_11555"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV61285.1"
                     /translation="MAGTATMERPVDAKAAPVDQDVLIVGAGISGIGMAVHLQMNCPD
                     RSFALVERREQLGGTWDLFRYPGIRSDSDMHTLGFIFEPWKHEKSIADGPAILEYLNR
                     IVDERHIRERIRFDRKVIGADWDSAAARWTVTMADGTGATSTTTARWLYLGSGYYDYD
                     EPFDAQFAGREDFQGQIIHPQFWPKDLDYQGKKVVVIGSGATAVTIVPSMKGAAHVTM
                     LQRTPTWYFIRPAKDGFANFLRKFLPEELAYKITRFKNVRLQDIA"
BASE COUNT          585 a          958 c          945 g          542 t
ORIGIN      
        1 gctcgcgcgg cgcgcgacgc cggcgccgat gatgctgccg gtgatcgtgt gcgtcgtcga
       61 caccggaatg ccgagcaggc tcgcggtgaa caccatgatc gacccgccgg tcgaggcggc
      121 gaaaccctga tggtgcgaca gcttggtgat gcggccgccc atcgtctcga tgatcttcca
      181 cccgcccgac agcgtcccca atgcgatcgc cacataacag ctgaacgcca cccagtgggg
      241 gatgtggaat tcgccgccga gatagccggt cgaatagagg agcacggtga tgatccccat
      301 cgtcttttgc gcatcgttga gcccgtggct gatcgaatag gccgccgaag acagaaggtg
      361 gagacggcgg aaggtcttct ccgcgaaatt ggcggtcgac cgccgcagcg cccagctgct
      421 gacgagcatc accagcatgg cgagcagcat gccgagcagc ggcgaaagga agatcgcgat
      481 gacggtcttg ttgagccccg tccactcgat gccctcgaac cccgcatgcg cgacgcccgc
      541 gccgacgatg ccgccgacga gcgcgtggct tgacgacgac ggaatgcctt tcagccaggt
      601 gacgacgttc cagaacatcg cgccgacgag cgcgccgaac acgacggctg gcgtcaccag
      661 atccttgtcg atgatcccct tgccgatcgt ctcggcgacc ttgtgcagct ccggaaagat
      721 gatcgacagg aaataggcag cgaagttgaa gaaggcggcg aacagcaccg cctgcaccgg
      781 ccgcaagagc cgcgtcgcga cgacggtcgc gatgctgttc gcggcgtcgt gcagaccgtt
      841 cagaaagtcg aacgccagcg caaggatgac gaggccgaca agaaggggga aagcgagttc
      901 gtgcatggtg ggcgcccccg cttatgcgtg gtcgatgacc aagccgtcga tttcgttcgc
      961 aacatcctcg aaactgtcgg tgacgcgttc gagatgacgg tagagttcgc gcccgatcag
     1021 gaagcggacg ctattcgact gcccatgctc cttgaagagg cgtttgaggc ctgcagcgtg
     1081 gatttcgtcg gcatggccct ccatccgcac cagccgttcg gtcagttcat gaaggcgcac
     1141 ggcattggcc gaaatgttgc ggagcagcgg cagcgcttcc gccgtcagcc tcgccgcgtc
     1201 gacgatgatg ccggcgatgt cgcgcatctc gggctcgaat tcggtgacgt cgtaaaggtc
     1261 gaccgcgccc gcggtcttct gcatctcgtc gatcgcatcg tccatcgagg cgatgaggtc
     1321 ggtgatcgcg ctgcggtcga acggggtcag gaaggtgcgg cggacggtct gcagcacttc
     1381 gcgcgtgatc gcgtcggcgt catgctcgcg ctcgatgatt tcctggacat ggtcggccat
     1441 gccctcgccg ccctgcagca tacgcgacaa ggcgttcgcc ccggcgacca gcgtcgcggc
     1501 gtggctttcg aacaattcaa aaaaatttcc ctgtcgcggc agcaaacgct gaaaccatgc
     1561 gaacatccag ctaacccctg tcttgtctgc tacgttttcg acgaccccgc gcggctgggc
     1621 gacggcgcga aaaccgctat cgccgaacga acggatcagc gcctgcaaat cgggttcttc
     1681 cacagccgat gcagcttcgc ggaacgagaa ccatttgcgg tcgcgttcgt ccatttcctt
     1741 ccagtcggcg agctcgttcg tcacggcgag cggaaagact tcgacattat acatgatcga
     1801 cgcgccgttc gcgcggcgct tcctatattc atagctgccg atcggcgtcg ggcagaccgc
     1861 tccgatgacg cccgcctctt cctcggcctc gaccgccgcg gcggcgtggc gcgtcatccc
     1921 gttcagcgga ttgcccttcg gcaccaccca gcgcttcgtc tcgcgcgagg tgatgagcag
     1981 gatttcggtc ggaccatcct gcgcagggcc gccgaatcga tagggaagga ccgcgatctg
     2041 acgcatttag atcaatcctt tcctgtggtt gatacggcgc gatgccagaa tgatgacaat
     2101 cgggtttcgc ccctatgaca ataatgtgac agccacaagg gacggcgcct cccgatcccc
     2161 tgttttcatg gctgtcaatt cgattgcaaa atgcaacgcg gtgcgtaagc tgccacccaa
     2221 aagcagacgg gagacgagat gatggcaggc acggcgacga tggaacgccc cgtggacgca
     2281 aaagctgcac cggtcgatca ggatgtgctg atcgtcggcg ccggcatttc gggcatcggc
     2341 atggccgtgc atctgcagat gaattgcccc gaccgcagct tcgcgctggt cgaacgccgc
     2401 gaacagcttg gcggcacctg ggatctcttc cgctatccgg gcatccgttc ggacagcgac
     2461 atgcacacgc tcggcttcat cttcgaaccc tggaagcatg agaaatcgat cgccgacggc
     2521 ccggcgatcc tcgaatatct gaatcgcatc gtcgacgagc gccatatccg cgagcgcatc
     2581 cgcttcgacc gcaaggtgat cggcgccgat tgggacagcg ccgccgcacg ctggacggtg
     2641 acgatggcgg acggcacggg cgcgacctcg acgaccaccg cgcgctggct ttatctcggg
     2701 tcgggttatt atgactatga cgagcccttc gatgcgcagt tcgcggggcg cgaggatttc
     2761 cagggccaga tcatccaccc gcaattctgg ccgaaggatc tcgactatca gggcaagaag
     2821 gtcgtcgtga tcgggtcggg cgcgaccgcg gtgacgatcg tcccctcgat gaagggcgcg
     2881 gcgcacgtca cgatgctgca gcgcacgccg acctggtatt tcatccgccc tgcgaaagac
     2941 ggcttcgcca acttcctccg caaattcctg cccgaagagc tcgcctacaa gatcacgcgc
     3001 ttcaagaatg tccggctgca ggacatcgcg
//
LOCUS       DATMSU010000432         6577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_92974, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000432 DATMSU010000000
VERSION     DATMSU010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92974"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VN034_11560"
     CDS             complement(<1..468)
                     /locus_tag="VN034_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HWV61286.1"
                     /translation="MTGVGKIHAIRVHDADNVATAIGDIAAGEAVFGDSGARASVAVE
                     RGHKIALAPIARGGAVVKYGFPIGTATADIAPGEHVHSHNLATALGSDGDYHYAGAPA
                     DTPPAATSSRHFSGYVRADGRVGTRNEIWILPTVGCVGNLAARVARIAGERHAG"
     gene            578..1360
                     /locus_tag="VN034_11565"
     CDS             578..1360
                     /locus_tag="VN034_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HWV61287.1"
                     /translation="MLATSDQENIARALIDARARKTPLTAYPGAMPQTMAEAYAIQDS
                     AIALDGRRVGGWKVGRIAPDLVARYGGNRLTGPIFADEIVGAAEQPAMPVYADGFAAA
                     EAEVLLCFGEVGTRDYDIDSVKDCIADVRTGIEIASSPFSGINRHGPAVTASDYGNNK
                     GLILGPPIADWRDADLIRMPVAMAIDGETVGTATMEAMLDGPFGSALFLIETLRARGI
                     AIPPGTWVSAGAITGIHEIRPGQRAEAIFGGKIRVGCSIRSF"
     gene            1382..2689
                     /locus_tag="VN034_11570"
     CDS             1382..2689
                     /locus_tag="VN034_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61288.1"
                     /translation="MAQAFADGGTAAPVPRSGRYRWLIVGVLFAATTVNYIDRTMLGL
                     LAPTLGDELKWTENDYGNIVSAFQFAYALGFLLMGWLIDRFGPKIGYAIAISIWTVGH
                     VAHGFASSIVSFMLARVILGVGEAGHFPSVVRASSEWFPQKERSYAIGWVNSATTIGV
                     ILTAPTVALFMRVLGFDWRETFIYTGAFGVLLLILWLWLYSNPRESGKVSEAELAWIE
                     HDPPEKIERLGWGAIVTKREAWAFAAAKFLTDPVWFLMLFWLPKYFSTTYDVDLKIVL
                     LPMIIMYLLSDAGSILGGWVSSKLIQAGHSVNFARKVTMVGAGLCVLPLLFVTGLDNM
                     WLAVVLIGIALAGHQAFSSTILSIPPDMFPKRAVGSVIGLGGFMGGVGGMIMAKSTGL
                     VLDATGGNYTLIFAACTTVYFLAVLAVHILSPRLAPVSVEGAK"
     gene            2694..4106
                     /gene="uxaC"
                     /locus_tag="VN034_11575"
     CDS             2694..4106
                     /gene="uxaC"
                     /locus_tag="VN034_11575"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HWV61289.1"
                     /translation="MPRPLLLSPDRLFPSDPAQRDIARRLYREVADLPIVSPHGHTDP
                     AWFAGNASFGNAAELLLHPDHYVFRMLYSQGISLDALGIRNRQADPRESWRIFAQNYH
                     LFRGTPSRMWMDWVFAEAFGFDVQFAAETSDLYYDRITEALATDAFRPRALFDRFGIE
                     VIATTESPLDTLEHHAAIRAANESGEWGGRVITAYRPDPVVDPEYEGFRDNLARFSEL
                     SGEDAFGYAGYLAAHRNRRAFFAEMGATSTDHGHPTAATADLPYAEAEALFARVTSAD
                     VSAADAELFRAHMLTVMAGMSLDDGLVMQIHPGAFRNHNPWLFANYGRDKGADIPMPT
                     DYVHALRPLLGRYGNEADLTIILFTLDETSYARELAPLAGHYPALKLGPAWWFHDSPE
                     GMRRFRGAMTETAGFYNSVGFNDDTRAFLSIPARHDVARRIDCGFLAQLVAEHRMEEW
                     EAAELATDLSYNLAKAAYKL"
     gene            4103..5425
                     /locus_tag="VN034_11580"
     CDS             4103..5425
                     /locus_tag="VN034_11580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HWV61290.1"
                     /translation="MRLSAQTPIPASVQAPGYDRAAQAAGIVHIGIGAFHRAHQAVYT
                     DDAMGAGDRDWGIVGVSLRSGDVAAQLGPQDGLYTVSTRSAAGTRLRLIGAVQKVLVA
                     ADDSQAVIDAIAAPTTHIVSFTVTEKGYLRRADGSLDLAAAVGKPSLYRFLAAGLAGR
                     KAAGLGGLTLLSCDNLAGNGAVLRRLMREYLAAYHPDLSAWFDGECTCPATMIDRIVP
                     ATADADRAAVEAALGARDEGAVVTEAFSQWVIEDDFAGPRPRWEAVGAELVADVAPYE
                     TAKLRMLNGAHSALAYIGLGRGHAFVHQAIADPEIRPVIERLMLGEAAPTIDAAPGQD
                     LAAYADALLGRFANPALDHRLIQIAMDGSQKIPQRWLETLAWHQARGARCPSLEEAIA
                     AWTAFLRSGHTIDDPLADKLREAAAGPDAMAKLFGNGGLIASEWRPEI"
     gene            complement(5530..6417)
                     /locus_tag="VN034_11585"
     CDS             complement(5530..6417)
                     /locus_tag="VN034_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV61291.1"
                     /translation="MDWDKLQYFLFVARHGTLARAGAALHVDATTVSRQVSALEAALQ
                     QTLFERAPTGFVLTAAGRALVPHAEAMAAAAARIHSEREGGSGLSGQLRVSVSEGFGN
                     SFIAPRLGRFVAAHPELEIDLVASSGFLNPSRREADMAVLLARPRKGPLITRKLSDYS
                     LGLYSPADRPDWQAAVAAVPLSRAGIPVIGYMPDILYAPELDYLGEIEPGLRANVRSS
                     SILAQRRMIAGGAGVGVLPCFLAAGDPELVRVRDDQVIARAFWLALHRDVAPQPRIRA
                     FIDWLDAEVRESRGLLVPA"
BASE COUNT         1096 a         2233 c         2139 g         1109 t
ORIGIN      
        1 tcccgcatgg cgttcgcccg cgatgcgcgc gacgcgcgcg gcgagattgc cgacgcagcc
       61 gacggtcggc aggatccaga tttcgttgcg cgtcccgacg cggccgtcgg cgcggacata
      121 gcccgaaaag tggcgcgacg aagtggcggc cggcggcgtg tcggccggcg ccccggcata
      181 atgatagtcg ccatcgctgc cgagcgcggt cgccagattg tggctgtgca catgctcgcc
      241 gggcgcgatg tcggcggtcg cggtgccgat cggaaagcca tatttgacca ccgcgccgcc
      301 gcgcgcgatc ggcgcgagcg cgatcttgtg tccgcgttcg acggcgaccg atgcgcgagc
      361 gccgctgtcg ccgaagaccg cctcgcccgc agcgatatcg ccaatcgcgg tcgcgacatt
      421 gtccgcgtca tgaacgcgga tcgcatggat ttttccgacg ccggtcatcg tcgctcagca
      481 cccggcatgg cgaagcaatt tgtcatacat ccgatattcg cttgcctttg acaccggtgt
      541 ctcttacacc gcatcgcgaa atagggaaat gggaaagatg ctggcgacaa gcgaccaaga
      601 gaatatagcg cgggccctga tcgacgcgcg tgcgcgaaag acaccgctta ccgcctatcc
      661 cggcgcgatg ccgcagacga tggccgaggc ctatgcgatc caggacagcg cgatcgcgct
      721 tgacggccgc cgtgtcggcg gatggaaggt cgggcgaatc gctcccgatc tcgtggcccg
      781 ctacggcggc aaccgcctga ccggcccgat cttcgccgac gaaatcgtgg gtgccgccga
      841 acagccggcg atgccggtct atgccgatgg attcgccgcg gccgaggccg aagtgctgct
      901 ctgcttcggt gaggtcggca cgcgcgacta tgatatcgac agcgtgaagg actgtatcgc
      961 cgacgtgcgc accggcatcg agatcgcgag ttcgcccttt tccgggatca accgccacgg
     1021 ccccgcggtc accgcgtcgg actatggcaa caacaagggg ctgatcctcg gcccgccgat
     1081 cgccgactgg cgggacgccg atctgatccg catgccggtc gcaatggcga tcgacggcga
     1141 aaccgtcggc acggcgacga tggaggccat gctcgacggc cccttcggct cggcgctgtt
     1201 cctgatcgag accttgcgcg cgcgcggcat agctattcca cccggaacat gggtgtccgc
     1261 gggggcgatc accggtattc atgaaatcag gccgggccag cgtgccgaag caatcttcgg
     1321 cggaaaaatc cgcgtcggct gctcgatcag aagcttttag agcaaataac aggagaggat
     1381 tatggcacag gcattcgcgg acggaggaac ggcggcgccg gtgcctcggt cggggcgtta
     1441 ccgctggctg atcgtcgggg tgctgttcgc ggcgacgacg gtcaactata tcgaccgcac
     1501 catgctcggg ctgctcgcgc cgaccctcgg cgacgagctc aagtggaccg agaacgacta
     1561 tggcaacatc gtctccgcct tccagttcgc ctatgcgctg ggctttctcc tcatgggctg
     1621 gctgatcgac cgcttcggtc ccaagatcgg ctatgcgatc gcgatctcga tctggaccgt
     1681 cggccatgtc gcgcatggtt tcgcatcctc gatcgtctcc ttcatgctcg cgcgcgtcat
     1741 cctcggcgtc ggcgaggcgg ggcattttcc ctcggtcgtc cgcgcgtcga gcgaatggtt
     1801 cccgcaaaag gaacgctcct atgcgatcgg ctgggtcaac agcgcgacta cgatcggcgt
     1861 catcctgact gcgccgacgg tcgcgctgtt catgcgcgtg ctcggcttcg actggcgcga
     1921 aaccttcatc tacaccggcg cctttggcgt tctgctgctg atcctctggc tctggcttta
     1981 cagcaacccg cgcgaaagcg gaaaggtcag cgaggccgag ctcgcgtgga tcgagcatga
     2041 tccacccgaa aagatcgagc ggctcggctg gggcgccatc gtcaccaagc gcgaggcgtg
     2101 ggcgttcgcg gccgccaaat tcctgaccga cccggtgtgg ttcctgatgc tcttctggct
     2161 gcccaaatat ttcagcacca cctatgacgt cgatctgaag atcgtgctgc tgccgatgat
     2221 catcatgtat ctgctctccg acgccggcag catcctcggc ggctgggtgt cgtcgaagct
     2281 catccaggcg ggccacagcg tcaatttcgc gcgcaaggtc acgatggtcg gcgcgggcct
     2341 ctgcgtgctg ccgctgctgt tcgtcaccgg gctcgacaat atgtggctcg cggtcgtgct
     2401 gatcggcatc gcgctcgccg ggcaccaggc cttttcgtcg acgatcctgt cgattccgcc
     2461 cgacatgttc cccaaacgcg cggtcggatc ggtgatcggg ctcggcggct tcatgggcgg
     2521 cgtcggcggc atgatcatgg cgaagtcgac cgggctcgtg ctcgacgcga cggggggcaa
     2581 ttacacgctc atcttcgccg cctgcacgac cgtctatttc ctcgccgtgc tcgcggtcca
     2641 catcctcagc ccgcgccttg cgccggtcag cgtggaaggg gcaaaataag ccgatgccgc
     2701 gtccgctgct tctctccccc gaccgcctct ttccgtccga tccggcgcag cgcgacatcg
     2761 cgcgccggct ctatagggag gtggcggacc tgccgatcgt cagcccgcat gggcacaccg
     2821 atccggcatg gttcgcgggc aacgcgtcct tcggcaatgc ggcggagctg ctgctccatc
     2881 ccgaccatta tgtgttccgg atgctctatt cgcagggcat ttcgctcgat gcgctcggca
     2941 tccggaacag gcaggcggac ccgcgcgaaa gctggcggat cttcgcgcaa aattatcatc
     3001 tctttcgcgg caccccgtcg cggatgtgga tggattgggt gttcgccgag gcgttcggct
     3061 tcgacgtgca attcgcggcc gaaacgtcgg acctctatta cgatcgcatc accgaggcgc
     3121 tggcgaccga cgcctttcgg ccgcgcgcgc tcttcgatcg cttcgggatc gaggtgatcg
     3181 cgaccaccga aagcccgctc gacacgctcg aacatcatgc cgcgatccgc gctgcgaacg
     3241 aaagcggcga atggggcggg cgcgtgatca ccgcctatcg ccccgatccg gtcgtcgacc
     3301 cggaatatga gggctttcgc gacaatctcg cgcgcttctc cgaactttcg ggcgaggatg
     3361 cgttcggcta tgccggctat ctggcggcgc accgcaatcg ccgcgccttc ttcgcggaga
     3421 tgggcgcgac ctcgaccgat cacggccacc cgaccgcggc gaccgccgac ctgccctatg
     3481 ccgaagccga agccctgttc gcgcgtgtca cgagcgctga cgtcagcgcc gccgatgccg
     3541 agctgttccg cgcgcatatg ctgaccgtga tggcggggat gagcctcgac gacggcctcg
     3601 tcatgcagat ccaccccggc gccttccgca accacaaccc ctggctgttc gccaactatg
     3661 gccgcgacaa gggtgccgat attccgatgc cgaccgacta tgtccacgcg ctacgtccgc
     3721 tgctcgggcg ttatggcaat gaagcggacc tcacgatcat cctcttcaca ttggacgaga
     3781 cgagctatgc gcgcgaactc gcgccgctcg cgggccatta ccccgcgctg aagctcggcc
     3841 ccgcctggtg gttccacgac agccccgaag gcatgcgccg cttccgtggg gcaatgaccg
     3901 agaccgcggg cttctacaac agcgtcggtt tcaacgacga cacgcgcgcc ttcctctcga
     3961 tccccgcgcg ccacgacgtc gcgcggcgca tcgactgcgg cttcctcgcg cagttggtcg
     4021 ccgagcaccg gatggaggaa tgggaggcgg ccgaactggc gaccgacctc agctacaatc
     4081 tcgcgaaagc ggcctacaag ctgtgaggct ctccgcgcaa acgcccatcc cggcgtcggt
     4141 gcaggcgccg ggatacgacc gcgccgccca ggcggcgggc atcgtccata tcggcatcgg
     4201 cgccttccac cgcgcgcatc aggcggtcta caccgacgat gcgatgggcg cgggcgaccg
     4261 cgactggggc atcgtcggcg tgtcgctgcg ctcgggcgac gtcgcggcgc agctgggtcc
     4321 gcaggatggg ctctataccg tcagcacgcg cagcgccgcg ggcacgcggc tccgcctgat
     4381 cggcgcggta cagaaagttc tcgtggcagc cgacgacagc caggcggtga tcgacgcgat
     4441 cgcggcgccg acgacgcata tcgtcagctt caccgtcacc gaaaaaggct atctgcgccg
     4501 cgccgacggt tcgctcgacc tcgctgcggc ggtcgggaag ccaagcctct atcgtttcct
     4561 cgccgccggg ctcgccgggc gcaaggcggc ggggctcggc gggctcacgc tgctcagctg
     4621 cgacaatctc gcgggcaatg gcgcggtgct gcgccgtttg atgcgcgaat atctcgccgc
     4681 ctatcacccc gacctgtcgg catggttcga cggcgaatgc acctgccccg cgacgatgat
     4741 cgaccgcatc gtccccgcca cggcagacgc cgaccgcgcc gcggtcgaag ccgcgctcgg
     4801 cgcgcgcgac gagggggcgg tagtcaccga agccttcagc caatgggtga tcgaggatga
     4861 tttcgccggc ccgcgcccgc gttgggaggc ggtcggcgcc gaactcgtcg ccgacgtcgc
     4921 accctatgag accgcgaagc tgcgtatgct caacggcgcg cattcggcgc tcgcctatat
     4981 cgggctcggc cgtggccacg cctttgtcca tcaggccatc gccgatccgg aaatccgacc
     5041 ggtcatcgag cggctgatgc tgggcgaagc cgcgccgacg atcgacgccg cgccggggca
     5101 agatctcgcc gcctatgccg atgcgttgct cggccgcttc gccaaccccg cgctcgacca
     5161 ccggctgatc cagatcgcga tggacggcag ccagaagatt ccgcagcgct ggctcgaaac
     5221 gctggcgtgg catcaggcgc gcggggcgcg ctgtccgtca ctcgaagaag cgatcgcggc
     5281 gtggaccgcc tttctacgga gcggccatac gatcgacgat ccgcttgcgg acaagctgcg
     5341 cgaagcggcg gccggtccgg atgcgatggc aaagctgttc ggcaacggcg ggttgatcgc
     5401 gtcggaatgg cggcccgaaa tctaacaacc ccgttcgcct gagcttgtcg aagggacgtt
     5461 tttccttctg acgccgttaa gaagatcggt gcttcgacaa gctcagcacg aacggatttg
     5521 aagtgggcgc taagccggaa ccaacaaccc ccgactttcc ctcacctccg catcgagcca
     5581 gtcgataaac gcgcggatgc gcggctgcgg cgcgacgtcg cggtggagcg cgagccagaa
     5641 ggcgcgcgcg atcacctgat cgtcccgcac gcgcaccagt tccggatcgc ccgcggcaag
     5701 aaagcacggc agcacgccga cccccgcgcc gcccgcgatc atgcgccgct gcgcgaggat
     5761 cgacgacgag cgcacattcg cgcgtagccc cggctcgatt tcgcccagat aatcgagttc
     5821 gggcgcataa aggatgtcgg gcatgtaacc gatcaccgga atccccgcgc gcgacagcgg
     5881 cacggcggcg accgccgcct gccagtcggg ccggtcggcg ggggagtaga ggccgaggct
     5941 gtagtccgac agcttgcggg tgatcagcgg gcctttgcgc ggtcgcgcga gcagcaccgc
     6001 catatcggcc tcgcgccgcg acgggttgag gaagcccgac gaggcgacga ggtcgatttc
     6061 gagctcggga tgcgccgcga cgaaccgccc gagccgcggc gcgatgaagc tgttgccgaa
     6121 gccttcggag acgctcaccc gcaattgccc cgacaatccc gaaccgcctt cgcgttccga
     6181 atggatgcgc gccgccgccg ccgccatcgc ctcggcgtgc ggaacgagcg cccggcctgc
     6241 ggcggtcaga acgaatccgg tcggcgcgcg ttcgaacaag gtctgctgga gcgccgcttc
     6301 gagcgcgctc acctgacggc tgaccgtcgt cgcatcgaca tgcagcgcgg ccccggcgcg
     6361 cgcgagcgtg ccgtggcgcg cgacgaacag gaaatattgc agcttgtccc agtccatgag
     6421 ctgcagatat gcaggataat cctgcaagtc tatcccttgc aatcatgccg caactggtct
     6481 gtggcagcgg caagacttta cgggagactc aagagctatg cgacagatcg accatcatat
     6541 cgtcggcggc gccggcggcg gcagccgctt cggcgac
//
LOCUS       DATMSU010000433        11747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_82859, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000433 DATMSU010000000
VERSION     DATMSU010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11747
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82859"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1906)
                     /locus_tag="VN034_11590"
     CDS             complement(65..1906)
                     /locus_tag="VN034_11590"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWV61292.1"
                     /translation="MDAIKKTLITGAAAIGMAGSANAADLSPTSWPAEIREAAEKAES
                     EGMAAQQSRVYAGKNGAISGTASAISVAAGLVTLQNGGNAADAATAVAMTGITTQFGS
                     VVSYAGIMSMTYYEAKTGKVYSLDGGYNSYRGETDPLTIPVADMGMLQAAARTGAAGG
                     GNLTTIPEAQKDLGRETLVGGYMAGMETMQRKFGKLEWADLFAPAIYYAENGVTLNKN
                     TAGFFTWRKSFLERTPEGKAFLAAGGTPEFKQGTKFAQPEAAKTLRAVASQGAQYMYS
                     GAWADEFVRIVGREGGKVVKADLADYGPIWSEPVSTSYAGTTVYAPGGDALASATFIP
                     LLNVAEVSGLNEKPPFWRDPQSFTEISRITGTLEGTPDLSKEVRAALDAKGIDYSLQA
                     RSTKEFAKRVAPLLPTIGVMPQGDGKSRHSNSLVVVDKDGNIAAITHTINSVVWGSTG
                     IVVGGIPIPDSAGFQQEALAKLTPGARLPNPMVQTIVLKKGKPILATAAIGSSLIPES
                     FKILLSVVGQKQSLADTQAAPALLTSFPLRAAIPEPSVRDVAVPAGAYDTAFGGALRA
                     KGLAVHDVPQAMAAGMRGTAATVAFDPKTKTWQATDTAGVLLFGAAY"
     gene            complement(1930..3633)
                     /locus_tag="VN034_11595"
     CDS             complement(1930..3633)
                     /locus_tag="VN034_11595"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWV61293.1"
                     /translation="MSPKNWPAGEYARFMAAQGVDRTTAGSAKGSNGAVTVAYGALAA
                     RAGLEALKQGGNAIDAAITTALAQVALTMGAPISYFGIMSLVYYDAKTGKTHTMNAEW
                     NTIAGETEPLTIPGAIDFSSTEALQGKGEPSGRTALVGGFMKGVEAAHKRFGKLPFAS
                     LFEPAIHIAEEGMPVSEMLDGIFKFRDRDIRRLPETRATLVKPDGSAYAEGDIFKQPK
                     LAATLRTVAAEGADYMYGGAWGEKLVAAVQADGGKMTLDDLKNYEVIWADPLVAELRR
                     GYSIQTNPAPNFGGFSIIEAQNLADAAGLCGGEHWTKSAGQLKKALEITQLFGISMFP
                     KEAQAAIYPGIDFGEAARVSKDHAAALWKQIAGGKTFTKWKRTTPMHSDDVVAVDAEG
                     NMAAITHSINCVNWGKTGIFIDGISIGDPASFQQAQIATVKPGARLPAPTETGILFKD
                     GVPVLAFASMGAGLHQRTFQGLLNVTCFDMDVEEAINTPDFFMPSTDPKTGETTLVVP
                     EGRFDHKLLDDTGYAWREMPLSEARLGGEGKWVAISRDPKTGELHAASHNRNNSDAVA
                     F"
     gene            4017..5327
                     /locus_tag="VN034_11600"
     CDS             4017..5327
                     /locus_tag="VN034_11600"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61294.1"
                     /translation="MAQNFDETGPLSDAAPKRDTRAWWALAVVTLVYAMNIADRFVLS
                     TLIEPIKAEFQLSDASVGFLTGVALAIFYTAAGLPLGALADRVNRRNMIMWALTIWSL
                     FTALCGMAQTFWQLLLARIGVGVGEAGGTPPSHSILADYFKPSERIVAMPIFTLGIAL
                     GSAIGGIGGGMLAEKFGWRHGLVVFAFASIPLLLLMLTVREPRRGASDVAALKDVTPS
                     LREALTFIRSQRALIHVVAGATIATFSGMGLIWWTPAFLARSHGFSVGDAGFEVGMMS
                     GLGGGAALVAATLITFKLARMAPKWQCHFLAWVTLLITIPGVLAHLVSDSRTALLLLW
                     LFIPFANVYVGPTLALLQNLVRPDMRGVTVAVLLFTANIANLAIAPQLIGLASDLIAT
                     RIADPSQSLSIALAISGLAGIWAALHFWAAIRSLPGDLVRAGTA"
     gene            complement(5338..6579)
                     /locus_tag="VN034_11605"
     CDS             complement(5338..6579)
                     /locus_tag="VN034_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWV61295.1"
                     /translation="MTITILENARVFDGINADCATGVSIAIEKGMIREVSDKAIAASD
                     ARRIDIGGRTLMPGLIDLHIHAYASDVNFQRVDAMGPAHRTAHAVRMLGHALDCGFTT
                     VRDIGGGDWSLWRSIEEGLIRGPRFLYAGRIVSMTGGHGDMRPMSVEGHHDHGFCQCG
                     QANSLTVIADGVDACLTAVREEFRRGAHTIKIMGSGGVASPTDPIWMNQYREDEVRAI
                     VGECVERRSYASAHCHPASAIRRCVEFGVRCIEHGTLIDDETAAFVAEKGAYIVPTMA
                     IIFALRELGPSLGLPGESQEKLDFVFDQALSGMDRMRNAGVKIGFGTDLLGTTYTRQC
                     TEFETRSEVFTPIEILRQATSIGAEVLQMEGKLGCVAADAFADLIVVDGDPLQDIGLL
                     ARNGGALPLIMRGGEIVKNSL"
     gene            complement(6648..8900)
                     /locus_tag="VN034_11610"
     CDS             complement(6648..8900)
                     /locus_tag="VN034_11610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61296.1"
                     /translation="MQPKWVIFTSCATIALTMPQLAAAQDSAPGSDMVDGNEIIVTAS
                     KRAEKLQDVPAAITAVTSEKIDTLGIRDFRDYATLVPGLSQRDNGAPGVGSIILRGLN
                     SGPQQVTNTTAVYIDETPFSASGFVSAGGLLTPSPELAEVERIEVLKGPQGTLYGASS
                     LGGLIRIVTKKPDASRFSGLASAEVSTTAHGEMGFMVRGSINAPIVRDQLAITATGYY
                     RRAGGFADNVRTGSKDVNRANLYGGRVALRYTPTTDLTFDISGIYQNIDSGGYAVETL
                     VPGTFQPAFGHYKYSNFDDFGSEIKYRIANANAEYRVGIGTFTASGSYARYKTRIETD
                     ATATYIPVARAVLGPLSETLFGAPIDTILPANSNARFYASPNMEKWTGEVRFASDRLG
                     PVEFMVGGFYTDEDNVYYTTVFAHDAEGALRAAPFDQLVKATTTSRYKEIAGFGNLTF
                     YITDTLDIQGGLRYAHNKQVAGTGGPGGITYYLPRVPRTFDFSDNSMSYLATLRWRPT
                     RNFSAFVRAASGYRPGGPQTNPAPPPGAQTFVRPDTVWNYEAGIKGSALDGALSFDIS
                     AFRIDWKDIQINSLFNGIVLQGNGASARVDGVELQLTARPSDLLTISGTMGHTNARLK
                     SIDPGASSATGARAGDKLPLTPAWTTSLIVDHRLLLGSTVEGNVGATLRFQSDMPATF
                     PALDPSRQNKLPEVTTLDLRASVTFDERFTVQLRADNLFDKVGYTNVGTDGGFVIRPR
                     TVTLGASTKF"
     gene            complement(9082..10803)
                     /locus_tag="VN034_11615"
     CDS             complement(9082..10803)
                     /locus_tag="VN034_11615"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWV61297.1"
                     /translation="MPLTPVENFGAALRVIDDVWTLSSANSFLGENKAPPVRLPDIGI
                     AAAAERAATGRALLDRIAAIDVESLPHDLPLTLEIARRTAERWAREEDWYWLAFDPLG
                     AGFYAMFAPTAYCAGFFLNSFRAPLQAYPFLTPGDGDRYTALVRDYGRIVRQMLERTE
                     GQAERGIHMPRVQLEQAVPLIENFRAAAAATLTPDRARLGAVDADRLLTQIERAIAED
                     VTPAFDAFAAYLQRDDYAAKAPASVGLSQYPGGAELYAALVPLHTTLDLTPDQVHATG
                     LDRMADIQAQMRALLDEAGFAGSPAEYLERLQEDPKWRASGAEAVGAVFERYIDRIAP
                     HIDATFNFKPKADHGVVALPEALTASMTFGYYSPPTATDALGRYNFNAPNLSQGPLNH
                     IGALNYHELVPGHHFHLATQDENETLHPLRRKSFINAFNEGWAEYAATLAGEMGMYPE
                     PEERFGRLMMDAFLTSRLVVDTGMNAFGWPLEKARDYMRENAFMPETEIRSETVRYSC
                     DIPGQALAYKLGDTVIHNAREKMRAALGNRFDIRDFHDAVLKPGALPLPMVERQVDEQ
                     TQRIAAS"
     gene            11114..>11747
                     /locus_tag="VN034_11620"
     CDS             11114..>11747
                     /locus_tag="VN034_11620"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61298.1"
                     /translation="MSITSVASPKGTDVPAAKAPWELYGPGQRRTFLFVLFLTGTSNY
                     ADRNVIGVLLEPIKAEFGVSDTMLGLLTGLAFAIFYVTVGLPIAWLADRGNRQKIIVW
                     SITVWSIMTVLCGMAQNFWQLALARVGVGAGEAGAIPPAQSLIADYYPPEQRAGALGI
                     YMMSSMAGYIIGMVAGGWVAQHYGWREAFIVIGLPGLLLALASRFALREPR"
BASE COUNT         1908 a         3699 c         3881 g         2259 t
ORIGIN      
        1 gcccgctcat gtcacatttt tcgccgaact acaaagaaga catgcattgt tgcatgttta
       61 gcggtcagta ggcggcaccg aacagcagaa cgcccgcggt gtcggtcgcc tgccacgtct
      121 tcgtcttcgg atcgaaggcg accgttgcgg cggtgccgcg catgcccgcg gccatcgcct
      181 gcggcacgtc gtggacggcg agccccttcg ccctgagcgc gcccccgaaa gcagtgtcat
      241 aggcgccggc ggggaccgcg acatccctga ccgacggttc ggggatcgct gcgcgaagcg
      301 ggaagctggt cagcagcgcg ggcgcggcct gcgtgtcggc aagcgactgt ttctggccga
      361 cgacgctgag caatatcttg aaactttccg ggatcagcga cgatccgatc gcggcggtgg
      421 cgaggatggg cttgcccttt ttcagcacga tcgtctgcac catcgggttg ggcaggcgcg
      481 cgccgggcgt cagcttggcg agcgcttcct gctgaaaacc ggcggaatcg gggatgggga
      541 tgccgccgac cacgatcccg gtcgaccccc agacgaccga attgatcgtg tgggtgatcg
      601 cggcgatatt gccgtccttg tcgacgacga cgagcgagtt cgagtggcgc gatttgccgt
      661 cgccctgcgg catcacgccg atcgtcggca gcaggggcgc gacgcgcttc gcgaactcct
      721 tggtgctgcg tgcctggagc gaatagtcga tacccttcgc gtcgagtgcg gcgcggacct
      781 ccttgctgag gtcgggcgtg ccttcgagcg tgcccgtgat gcgcgaaatc tcggtgaagg
      841 attggggatc gcgccagaag ggtggcttct cgttgagccc cgagacctcg gcgacgttga
      901 gcagcgggat aaaggtcgcc gacgccagcg catcgccgcc aggggcatag accgtcgtcc
      961 cggcatagct ggtcgatacc ggctcgctcc agatcgggcc ataatcggcg aggtcggcct
     1021 tgacgacctt gccgccttcg cgaccgacga tccgcacgaa ctcgtcggcc caagcaccgc
     1081 tgtacatata ttgcgcgccc tgcgacgcta ccgcgcgcag cgtcttcgcg gcttcgggct
     1141 gggcgaattt tgtgccttgc ttgaattcgg gtgtgccccc tgcggcaagg aacgccttgc
     1201 cctcgggtgt gcgctcgagg aagcttttgc gccaggtgaa gaagcccgcg gtattcttgt
     1261 tcagcgtgac gccattttcg gcataataga tcgccggcgc gaagaggtcg gcccattcga
     1321 gtttgccgaa cttccgctgc atcgtctcca tgcccgccat atagccgccg acgagcgttt
     1381 cgcggccaag gtccttctgc gcctcgggga tcgttgtcag gttgccgccg cccgccgcgc
     1441 cggtgcgcgc cgcggcctga agcatgccca tgtcggcgac cgggatcgtc agcgggtcgg
     1501 tttcgccccg gtagctgtta tagcccccgt cgagcgaata gaccttgccg gtcttcgcct
     1561 cataataggt catcgacatg atgcccgcat aggacacgac cgacccgaac tgggtcgtga
     1621 tgcctgtcat cgcgacggcg gtcgcggcgt cggcggcgtt gccgccattc tgcagcgtga
     1681 caaggccggc cgcgaccgag atcgccgaag ccgtgcccga gatcgcgccg ttcttgcccg
     1741 catagacgcg cgattgttgc gcggccatgc cctcgctttc ggccttctcg gcggcttcgc
     1801 gaatttcggc cggccagctt gtcggtgaca ggtcggcggc gttcgccgaa cccgccatgc
     1861 cgatcgccgc cgcaccggta atgagcgtct ttttgatcgc gtccatatcc tgtcctcccc
     1921 tttgttgcgt cagaaggcga ccgcgtcact gttgttgcgg ttgtgcgatg cggcgtggag
     1981 ttcgccggtc ttcggatctc ggctgatcgc cacccatttt ccttcgccgc cgagccgcgc
     2041 ttcggacaac ggcatttcgc gccacgcata gccggtgtcg tcgagcagct tgtggtcgaa
     2101 acgcccctcg gggaccacca gcgtggtctc gccggtcttg ggatcggtcg acggcataaa
     2161 aaagtcgggc gtgttgatcg cttcctcgac gtccatgtcg aagcaggtga cgttgagcag
     2221 cccctgaaag gtgcgctgat ggaggccggc acccatcgag gcgaaggcga gcaccggaac
     2281 gccgtccttg aacaggatgc cggtttcggt aggggcgggg aggcgcgcgc cgggcttgac
     2341 cgtcgctatt tgagcctgct gaaacgaagc cgggtcgccg atcgagatgc cgtcgatgaa
     2401 gatgccggtc ttgccccagt tcacgcaatt gatcgaatgg gtgatcgcgg ccatattgcc
     2461 ttcggcgtcg accgcgacga cgtcgtccga gtgcatcggt gtcgtgcgct tccacttggt
     2521 gaaggtcttg ccgcctgcga tctgcttcca cagcgccgcg gcatggtctt tcgacacgcg
     2581 cgcggcctcg ccgaaatcga tgccgggata gatcgcggcc tgcgcttcct tcgggaacat
     2641 cgaaatgccg aagagctggg tgatttcgag cgccttcttc agttgcccgg ccgatttcgt
     2701 ccaatgctcg ccgccgcaca gtcccgccgc gtcggcaagg ttctgcgcct cgatgatcga
     2761 gaagccgccg aagttcggcg ccgggttggt ctggatcgaa tagccgcggc gcagctcggc
     2821 cacgagcggg tcggcccaga tcacttcgta attcttgagg tcgtcgagcg tcatcttgcc
     2881 gccgtcggcc tgcaccgcgg cgaccagctt ctcgccccat gcgccgccat acatatagtc
     2941 ggcgccctcg gccgcgacgg tgcggagcgt cgccgcgagc ttcggctgct tgaagatatc
     3001 gccttcggca taagcgctgc cgtcgggttt cacgagcgtc gcgcgcgttt cgggaaggcg
     3061 gcggatatcg cggtcgcgga acttgaagat gccgtcgagc atctcgctca ccggcatgcc
     3121 ctcctcggcg atatggatcg cgggctcgaa cagactggcg aagggcagtt tgccgaaacg
     3181 cttgtgcgcc gcctcgacgc ccttcatgaa gccgccgacg agcgcggttc gcccgctcgg
     3241 ttcgcccttg ccctggagcg cttcggtgct cgaaaaatcg atcgcgccgg ggatggtcag
     3301 cggttcggtt tcgcccgcaa tggtgttcca ctcggcgttc atcgtgtggg tcttgccggt
     3361 cttcgcgtca taatagacga gcgacatgat gccgaaatag ctgatcggcg cgcccatcgt
     3421 cagcgcgacc tgcgccagcg cggttgtaat tgcggcatcg atggcattgc cgccctgctt
     3481 gagcgcttcg agccccgcgc gtgcggcgag cgcgccatag gcgacggtga ccgcgccatt
     3541 actgcctttc gccgaacccg cggtggtgcg gtcgacgcct tgcgcggcca tgaagcgggc
     3601 gtattcgccg gcgggccagt tcttcggtga caagtcgatg cgtcctttcg ttgcggcgat
     3661 ggccttcgat gcgacgcccc ccagggaaga ggtggcggtc agggcggtcg ttgcagccaa
     3721 tgcggttatt gcggtgcgtc gatcaatgtt catgggctcc cctcttccat tccgtgtccg
     3781 cgcaatcgcg ccgatcatcc gtcgctcccg cttggcggga atcattttac atacatagtt
     3841 gtcttataaa atttcagcct ttgcaaccga tgattttgcg gaatgatcat tcttaaggct
     3901 aggaatatag ggaatagagg gatctcgccc cttcgatatt gcaaacatgc gatagtgcat
     3961 taaaagatcg ggcaagcccg cgctgaaatt gtcgggcaat tcggggaggc gattgcatgg
     4021 cgcagaattt cgacgagacc gggccattgt ccgacgcggc gccaaaacgc gacacgcggg
     4081 cctggtgggc gctcgcggtc gtcacgctcg tctatgcgat gaatatcgcc gaccgcttcg
     4141 tcctgtcgac gctgatcgaa ccgatcaagg ccgagttcca gctcagcgat gcttcggtcg
     4201 gcttcctgac cggggtggcg ctcgcgatct tctataccgc cgcggggctg ccgctcgggg
     4261 cgctggcgga ccgtgtcaac cgccgcaaca tgatcatgtg ggcgctgacg atctggtcgc
     4321 tctttaccgc gctttgcggg atggcgcaga ctttctggca attgctcctc gcgcggatcg
     4381 gcgtcggtgt gggggaggcg ggtggcaccc cgccatcgca ttcgatcctc gccgattatt
     4441 tcaaaccatc cgagcgcatc gtcgcgatgc cgatcttcac gctcggcatt gcgctcggct
     4501 cggcgatcgg cggaatcggc ggcgggatgc tcgcggagaa attcggctgg cgccacggtc
     4561 tcgtcgtctt tgccttcgcg agcatcccgc tgcttctcct gatgctcacg gtgcgcgagc
     4621 cgcgccgcgg cgcgagcgac gttgcggcgc tgaaggacgt gacgccaagc ctgcgcgagg
     4681 cgctgacctt catccggtcg cagcgcgcgc tcatccacgt cgtcgccggc gcgacgatcg
     4741 cgaccttctc gggcatgggg ctcatctggt ggaccccggc cttcctcgcg cgctcgcacg
     4801 gcttcagtgt cggcgacgcc gggttcgaag tcgggatgat gagcgggctc ggcggcggcg
     4861 cggcgctcgt cgcggcaacg ctgatcacct tcaagctcgc gcgcatggcg ccgaaatggc
     4921 aatgtcattt ccttgcctgg gtgacgctgc tgatcacgat tccgggcgtg ctcgcgcatc
     4981 tcgtcagcga ttccagaacg gctttgctgc tgctctggct cttcatcccc ttcgccaacg
     5041 tctatgtcgg gccgacgctg gcgctgctcc agaatctcgt ccgccccgac atgcgcggcg
     5101 tgaccgtcgc ggtgctgctc ttcaccgcga acatcgccaa tctcgcgatc gcgccgcagc
     5161 tgatcggcct cgcatccgac ctgatcgcga cgcggatcgc cgatccgtcg cagtcgctca
     5221 gcatcgcgct cgcgatttcg gggctggccg gcatctgggc ggcgctgcat ttctgggcgg
     5281 cgatccgttc gctgccgggc gatctggtcc gggcgggaac ggcctgagac aggtagttta
     5341 aaggctgttc ttgacgatct cgccgccgcg catgatcagc ggcagtgcgc cgccattgcg
     5401 ggcgagcagg ccgatatcct gcaggggatc gccatcaacg acgatcaggt cggcaaaggc
     5461 atcggcggcg acgcaaccga gcttgccctc catttgcagc acctcggcgc cgatcgacgt
     5521 cgcctgccgc aggatttcga tcggcgtaaa cacctcgctg cgcgtctcga actcggtgca
     5581 ctggcgcgta taggtcgtgc cgagcaggtc ggtgccgaaa ccgatcttga ccccggcatt
     5641 acgcatccgg tccatgcccg aaagcgcctg atcgaagacg aagtcgagct tttcctggct
     5701 ttcgcctggc agccccaacg acggcccgag ctcgcggagc gcgaagatga tcgccatcgt
     5761 cggcacgata taggcgccct tttcagcgac gaacgccgcc gtctcatcgt cgatcagcgt
     5821 gccatgctcg atgcagcgta cgccgaattc gacgcagcgg cggattgcgc tcgccgggtg
     5881 gcaatgcgcc gaggcgtagc ttcgccgttc gacgcattcg cccacaatcg cgcggacttc
     5941 gtcttcgcga tactggttca tccagatcgg gtcggtaggc gacgcgaccc cgcccgaccc
     6001 catgatcttg atcgtatgcg cgccgcgacg aaactcctcg cgcacggcgg tcaagcaggc
     6061 gtcgaccccg tcggcgatca ccgtcaggct gttcgcctgc ccacactggc aaaagccatg
     6121 gtcgtggtgc ccctcgacgc tcatcgggcg catgtcgccg tggccgccgg tcatcgacac
     6181 gatccgcccg gcatagagaa agcgtgggcc gcggatcagc ccttcctcga tcgagcgcca
     6241 cagcgaccag tcgccgccac cgatatcgcg cacggtggta aagccgcagt cgagcgcatg
     6301 gccgagcatg cggacggcat gcgcggtgcg gtgcgccggc cccatcgcgt cgacgcgctg
     6361 gaaattcaca tcggaggcat aggcgtggat gtgaaggtcg atcaggcccg gcatcagcgt
     6421 ccgcccgccg atgtcgatgc ggcgcgcgtc ggatgcggcg atcgccttgt ccgacacctc
     6481 gcggatcatc cctttctcga tcgcgatcga cacgccggtc gcgcaatcgg cattaatgcc
     6541 gtcgaagacg cgggcgtttt ccaggatggt gatggtcatg acgatgcctc ggacaaggga
     6601 gttgatgacc gggcggcggc tccggaaagc cgccgcccgc gggacaatca gaacttcgtc
     6661 gaagcgccga gcgtcaccgt gcgcgggcga atgacgaagc cgccgtcggt gccgacattg
     6721 gtatagccga ccttgtcgaa cagattgtcg gcgcgcagct gtacggtgaa gcgctcgtcg
     6781 aaggtcacgc tggcgcgcag atcgagtgtc gtcacttcgg gcagcttgtt ctgccgcgac
     6841 gggtcgagcg ccgggaaggt agccggcatg tccgactgga aacgcagcgt cgcgccgaca
     6901 ttcccctcca ccgtgctgcc gagaagcagg cgatggtcga cgatcagcga ggtcgtccag
     6961 gccggcgtca ggggcagctt gtcgcccgcc cgcgcgcccg tcgccgacga agcgccggga
     7021 tcgatcgatt tgagccgggc gttggtgtgt cccatcgtgc cgctgatcgt cagcagatcc
     7081 gacgggcgcg ccgtcagctg cagttcgacg ccgtcgactc tcgcgctcgc gccattgccc
     7141 tgcagcacga tgccgttgaa cagcgagttg atctggatat ccttccagtc gatgcggaag
     7201 gccgaaatgt cgaacgacag cgcgccgtcg agcgccgagc ccttgatgcc ggcctcatag
     7261 ttccacaccg tgtcggggcg cacgaaggtc tgtgcgcccg gcggcggggc agggttggtc
     7321 tgcgggccgc cggggcgata gccgctcgcc gcgcggacga aggcgctgaa gttgcgggtc
     7381 gggcgccagc ggagcgtcgc gagatagctc atcgaattgt cgctgaagtc gaacgtgcgc
     7441 ggaacccgcg gcagataata ggtgatcccg ccggggccgc cggtgcccgc cacctgtttg
     7501 ttgtgcgcgt aacgcaggcc gccctgaata tcgagcgtat cggtgatgta aaaggtcagg
     7561 ttgccgaagc ctgcgatctc cttgtagcgc gatgtcgtcg tcgccttgac gagctgatcg
     7621 aacggcgcgg cgcggagcgc gccctcggca tcgtgcgcaa agacggtggt ataatagaca
     7681 ttgtcttcgt cggtgtagaa gccgccgacc atgaactcga ccgggccgag ccggtccgag
     7741 gcgaagcgca cttcgccggt ccatttttcc atgttcggcg aggcatagaa gcgcgcgttg
     7801 gaattggcgg gcaggatcgt atcgatcggc gcgccgaaca gggtctccga cagcgggccg
     7861 agtacggcgc gtgcgaccgg gatataggtc gcggtcgcat cggtctcgat gcgcgtcttg
     7921 tagcgcgcat agctgcccga agcggtgaag gtcccgatgc cgacgcgata ttcggcgttg
     7981 gcgttggcga tgcgatattt gatctccgac ccgaaatcgt cgaagttcga atatttatag
     8041 tgtccgaacg ccggctggaa cgtgcccggc acgagcgttt cgacggcata gccgccgctg
     8101 tcgatattct ggtagatgcc gctgatgtcg aaggtgagat cggttgtggg cgtatagcgc
     8161 agcgcgacgc ggccgccgta gaggttcgcg cggttgacgt ctttcgatcc ggtgcggaca
     8221 ttgtcggcga agccgccggc gcggcgataa tagcccgttg cggtgatcgc gagctggtcc
     8281 ctgacgatcg gcgcgttgat cgaaccgcgg accatgaagc ccatctcgcc gtgcgcggtg
     8341 gtcgacacct cggccgaagc gaggcccgaa aaacgcgacg catcgggctt tttggtgacg
     8401 atgcggatca ggccgccaag gctgctcgcg ccatagagcg tcccttgcgg tcccttcagc
     8461 acctcgatcc gctcgacttc ggcgagctcg ggcgagggag tcagcaggcc gccggcggac
     8521 acgaagccgc tcgccgagaa gggcgtttcg tcgatgtaga ccgccgtcgt gttggtgacc
     8581 tgctgcggac ccgaattgag gccgcgcagg atgatcgagc cgacgccggg tgcgccattg
     8641 tcgcgctggc tgagccccgg gacgagcgtc gcataatcgc ggaaatcgcg aatgccgagc
     8701 gtatcgatct tctccgacgt caccgcggtg atcgcggcgg gcacgtcctg cagcttttcg
     8761 gcgcgcttgc tcgcggtcac gatgatttcg ttgccgtcga ccatatcgga ccccggcgcg
     8821 ctgtcctgcg ctgccgccag ttggggcatg gtgagcgcga tcgtcgcgca cgaggtgaaa
     8881 atgacccatt tcggctgcat cgtgaatctc cctgacgatg gattcgttcc tctgcgactt
     8941 gagtcgctat ttaatgcaga actgttcact attgtgctgt cgattgcaac cgcgtttttt
     9001 gcatgcttat tgcatccgca atgggcttga tgacgcgaat cagctgtgca cgaaagtgca
     9061 gcggccttca tgccgtcgcg atcagctcgc ggcgatacgt tgcgtctgtt cgtccacctg
     9121 ccgttcgacc atcggaagcg gcagcgcgcc gggtttcagg accgcgtcat gaaaatcgcg
     9181 gatgtcgaag cggttgccga gcgcggcgcg catcttttcg cgcgcattgt gaatgacggt
     9241 atcgccgagc ttgtaggcga gcgcctgtcc cggaatatcg cacgagtagc gaacggtttc
     9301 cgaccggatt tcggtttcgg gcatgaaggc gttttcgcgc atataatcgc gcgccttttc
     9361 gagcggccag ccgaacgcgt tcatccccgt atcgaccacc aggcggctcg tcaggaaagc
     9421 atccatcatg agccgcccga agcgctcctc gggttcggga tacatgccca tttcgcccgc
     9481 gagcgttgcg gcatattcgg cccagccttc gttgaacgcg ttgatgaagg acttgcggcg
     9541 cagcggatgc agcgtctcat tctcgtcctg cgtcgcgagg tggaaatgat ggcccggaac
     9601 gagttcgtga taattgagcg cgccgatatg gttgagcggt ccctgcgaca ggttgggcgc
     9661 attgaaatta tagcgcccga gcgcgtcggt cgcggtgggc gggctgtaat agccgaaggt
     9721 catcgacgcg gtgagcgctt cgggcagcgc gacgacgcca tggtcggcct tgggcttgaa
     9781 gttgaacgtc gcgtcgatat gcggcgcgat ccggtcgata tagcgttcga acaccgcgcc
     9841 gacggcttcg gcacccgatg cgcgccactt cggatcctcc tgcagccgct cgagatattc
     9901 ggccgggctt ccggcaaaac ccgcttcgtc gagaagcgcg cgcatctgcg cctgaatgtc
     9961 ggccatgcga tcgaggccgg tggcgtggac ctgatcgggc gtcaggtcga gcgtggtgtg
    10021 cagcgggacg agggcggcat atagttccgc cccgcccgga tattgcgaga ggccgacgct
    10081 cgcgggcgcc ttcgccgcat aatcgtcacg ctgcagataa gccgcgaacg catcgaaggc
    10141 gggggtgacg tcctcggcga tcgcccgttc gatctgggtg agaagccgat cggcgtcgac
    10201 tgcgccgagc cgggcgcggt cgggcgtcaa cgttgccgcg gcagccgcgc ggaaattctc
    10261 gatcagcggc accgcctgtt cgagttgcac gcgcggcata tggatcccgc gctcggcctg
    10321 gccctccgtc cgctcgagca tctgccggac gatacggcca tagtcacgaa ccagcgccgt
    10381 gtagcgatcg ccgtcgcccg gcgtcaggaa cggataggcc tggagcggcg cgcggaacga
    10441 attcaggaaa aagccggcgc aataggccgt gggcgcgaac atcgcgtaga atccggcgcc
    10501 cagcggatcg aatgcaagcc agtaccagtc ttcctcgcgt gcccagcgct cggcggtgcg
    10561 gcgcgcgatt tcaagcgtca ggggcaggtc gtgcggcagg ctctcgacat cgatcgcggc
    10621 gatgcggtcg agcagagcgc gtccggtcgc cgcgcgttcg gcggcggcgg cgatcccgat
    10681 gtcgggcagc cggacgggag gcgccttgtt ttcgccgaga aagctgtttg ccgaagagag
    10741 ggtccagaca tcgtcgatca cgcgcaaggc agcaccgaaa ttctcgacag gcgtgagcgg
    10801 catgatctgt tccttcccca tatggcgtta gcgccggtcg ggccgtatcg cgcagcccgg
    10861 cgacattgaa tgcgtatctg tatgtttcat tcctgacgca agatggcaag gctttctgat
    10921 atgcctcggc gcatccctgg cgacatccgg cttggccgtc gcgcggcaag cgcgcaaaaa
    10981 ccggggaaaa aggcgctctc aaataaaaat gcatttatgt attgaatatg cgaaatcttg
    11041 ccgatactcg ttgcgaatgc gggtcaagcg atgaaagcgc ggacctgccc ggagtcgtcg
    11101 cggagaggtg atgatgagca taacaagtgt tgcatcgccg aagggtaccg acgtgcccgc
    11161 cgccaaggcg ccgtgggaac tttatggacc cggccagcgc cggacctttc tcttcgtcct
    11221 cttcctgacc ggcacctcca actatgccga ccgcaacgtc atcggcgtgc tgctcgagcc
    11281 gatcaaggcc gaatttggcg tatccgatac catgctcggc ctgcttacag gcctcgcctt
    11341 cgcgatcttc tacgtcaccg tcggcctccc gatcgcctgg ctggccgacc gcggcaaccg
    11401 gcaaaagatc atcgtctggt cgatcacggt gtggagcatc atgaccgtcc tgtgcggcat
    11461 ggcgcagaat ttctggcaac tcgcgctcgc gcgggtcggc gtcggggcag gggaggcggg
    11521 cgcgatcccg ccggcgcaga gtctgatcgc cgactattac ccgcccgaac agcgcgccgg
    11581 cgcgctgggt atctatatga tgtcgtcgat ggccggctac attatcggca tggtcgcagg
    11641 cggctgggtc gcgcagcatt atggctggcg cgaagccttc atcgtcatcg gcctgccggg
    11701 cctgctgctc gcgctggcgt cgcgcttcgc gctgcgcgag ccgcgcc
//
LOCUS       DATMSU010000434         4250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_168104,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000434 DATMSU010000000
VERSION     DATMSU010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168104"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..779)
                     /locus_tag="VN034_11625"
     CDS             complement(171..779)
                     /locus_tag="VN034_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWV61299.1"
                     /translation="MSIVKKRLSPEESRSAALEAARQILVEMGPAAVTLKAVAARIDR
                     THANLLHHFGSAAGLQKALAAYQAETVCDTIGKKMAESPPGERNVREIVDLAFDAFNE
                     GGAGALATWMAATGNDDALDPIVDAIHRLIDGMAPDAHEKRLMHEDTLALVLMALGDA
                     QLGGPMAEALGLPRDTSRVLATELITGRIAKFWAEHGGKPAS"
     gene            838..1728
                     /locus_tag="VN034_11630"
     CDS             838..1728
                     /locus_tag="VN034_11630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWV61300.1"
                     /translation="MSSLSRSPTPADLSITPRDMRFGRDDRQGRWWLNGDPIASAFHT
                     ALSVTFPRGEAMFIEAVKAHRDGVPDKLAREIRAFTQQEVIHSREHVVFNKKAAEAGY
                     DLSELEDDVNQVLDLIKTRPQIVNLMATIALEHYTAMMAAVMLREDSMYAGAEPEWAA
                     LWKWHAIEEIEHKGVAYDTWLHATKDWSRFRRWRAKSLMMLLVTSRFWPKRVKGMKAL
                     LRQDGLTGWRVTARIWWYLLGTPGVLRKSFFPWLSYFMPGFHPWNHDDRSLIQKYESD
                     YADAIMPAHSSKPELAVAAA"
     gene            1863..2423
                     /locus_tag="VN034_11635"
     CDS             1863..2423
                     /locus_tag="VN034_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61301.1"
                     /translation="MRPHLTAAEIAQIELQLAGLSSLLEFGCGEATLVAARQVRRIVS
                     VDGDPAWLGKMQAEVAREAVEFTPVHIDIGPVGEGGYPAGESRIRDWPRYHSHIWRGM
                     GGSPDAVSIAGRFRVACLLQAIIHCKPDCVFLFRDFNDEPHYHAVLRHVEVLTRVDGL
                     GVLRAKRQVDGTAVLHDLFDHYLDPD"
     gene            2456..2998
                     /locus_tag="VN034_11640"
     CDS             2456..2998
                     /locus_tag="VN034_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV61302.1"
                     /translation="MASETFSAPPTIETDRLRLRPGRLADKDVHITMWADERVTRFIG
                     GEPRAPDVSWGKFLSSAGLWPVMGFGYWVFADRASDALIGMGGLSYFCRGIPELEGRP
                     EAGWAFDADHWGAGYATEAMTAALNWADANLDATDVRCIIDLGNDASERVAAKLGFRR
                     IGESDALGHVVAIYSRPRGG"
     gene            3092..3655
                     /locus_tag="VN034_11645"
     CDS             3092..3655
                     /locus_tag="VN034_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61303.1"
                     /translation="MRSKMFTLAGAAALALGACTVQEDKDKAAGPEVAESGGVAREAM
                     KAEEPEGPPVTPMPAATVIQSQPGPDGSQVDLLSVKVTGDILTVTLRCSSQEKHNRES
                     LRVAQISVIDDATSQRIGVLKDNEGGAMVSNLNRSSNSDYDTMGVDCTSKPGVMWAKF
                     AAPPATSKTVSINFPNIAPFDGIAVQR"
     gene            3652..4242
                     /locus_tag="VN034_11650"
     CDS             3652..4242
                     /locus_tag="VN034_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV61304.1"
                     /translation="MRRAIASLGTVALLAGCSGNAPENVAGEGERQAAPTAVVSETDP
                     TPPAPGLRAEVSPLSGKLSGLNTRVTDMGTIIDLPSDALFEYDKAALTPAAEVELRKA
                     AELIRASPSGAIEIIGHTDSKGDDSYNLTLSEARARTVADWFGRQAGVRQREFRVSGK
                     GESAPIVPNEDAGGADDPAGRAKNRRVEVVVPTAAG"
BASE COUNT          698 a         1392 c         1454 g          706 t
ORIGIN      
        1 gttgaggtcg tgagctgatt tcccctcccg ccggcgggag gggcagcgag acttacgaac
       61 tttgttcgtt agtcgcagcg gggtgggcat tcgcaccgtt gctggcccac ccccagcccc
      121 tcccgcaagc gggaggggag atgaaggccc gcttccggcc gaaaccgccg tcagctcgcc
      181 ggctttccgc catgttcggc ccagaatttc gcgatgcgtc cggtgatgag ttcggtcgcg
      241 agcacgcgcg aggtgtcgcg cggcaggccg agcgcctcgg ccatcgggcc gccgagctgc
      301 gcatcgccca aggccatcag caccagcgcg agcgtatcct cgtgcatcag gcgtttttca
      361 tgcgcgtcgg gcgccatgcc gtcgatcagg cggtggatcg catcgacgat cgggtcgagc
      421 gcgtcgtcgt tgcccgtcgc cgccatccac gtcgcgagcg cgcccgctcc gccttcgttg
      481 aacgcatcga aagcgaggtc gacgatctcg cgaacattgc gttcgcccgg cggcgattcg
      541 gccattttct tgccgatcgt gtcgcacacg gtttcggcct gataggcggc gagcgccttt
      601 tgcagccccg cggcgctgcc gaaatggtgg agcagattgg cgtgggtgcg gtcgatccgc
      661 gccgcgacgg ccttcagcgt caccgccgcc ggccccatct cgaccaggat ctggcgcgcg
      721 gcctcgagcg ccgccgaccg gctttcctcg ggactcaaac gcttcttcac tattgacata
      781 tatgtaagta aaccctattt gctggccccg acaacccttt tcgacggtat gagccatatg
      841 tccagccttt cccggtcgcc gacccccgcc gatctttcga tcaccccgcg cgatatgcgc
      901 ttcggccgcg acgaccggca ggggcgctgg tggctgaacg gcgatccgat cgcctcggcc
      961 ttccacaccg cgctgtcggt gaccttcccg cgcggcgagg cgatgttcat cgaggcggtg
     1021 aaggcgcacc gcgacggggt tcccgacaag ttggcgcgcg aaatccgcgc ctttacgcag
     1081 caggaagtga tccacagccg cgagcatgtc gtcttcaaca agaaggcggc ggaggcggga
     1141 tatgacctca gcgagctcga ggacgacgtc aaccaggtgc tcgacctgat caagacgcgc
     1201 ccgcagatcg tcaacctgat ggcgacgatc gcgctcgaac attataccgc gatgatggcc
     1261 gcggtgatgc tgcgcgaaga cagcatgtat gcgggcgccg agcccgaatg ggccgccttg
     1321 tggaaatggc acgcgatcga ggagatcgag cataagggcg tcgcgtacga cacctggctc
     1381 cacgcgacga aggactggag ccgtttcagg cgctggcgcg ccaagtcgct gatgatgctg
     1441 ctcgtcacct cgcgtttctg gcccaagcgc gtgaagggga tgaaggcgct gctccggcag
     1501 gacggcctca ctggctggcg cgtaacggcg cgtatctggt ggtatctgct cggtacgccg
     1561 ggcgtgctgc gcaagagctt ctttccctgg ctttcctatt tcatgccggg tttccacccg
     1621 tggaaccacg acgaccggtc gctgatccag aaatatgaaa gcgactatgc cgacgcgatc
     1681 atgccggcgc attcatcgaa acccgaactg gctgtcgcgg cagcctgacc cgcgaaggcg
     1741 ggcacccgga gcggtcttct ctctcccctc tccgggggcc cgccttcgcg gcgataatgt
     1801 gccgatcgcg gctggacgcc gcggcgcccg tcccatatcg ctgagcgggt ggggagggga
     1861 ccatgcgacc acatctgacc gccgccgaaa tcgcgcagat cgagctgcag cttgcggggc
     1921 tgtcttcgct gctcgaattc ggctgcggcg aagccacgct ggtcgcggcg cggcaggtgc
     1981 ggcggatcgt cagcgtcgac ggcgatcccg cctggctcgg caagatgcag gccgaggtgg
     2041 cgcgcgaagc ggtcgaattc accccggtcc acatcgacat cggaccggtg ggcgaggggg
     2101 gctatcccgc cggcgaaagc cgcatccgcg actggccgcg ctatcatagc catatttggc
     2161 gcggcatggg cggcagtccc gatgcggtgt cgatcgccgg gcgttttcgc gtcgcctgcc
     2221 tgttgcaggc gatcatccac tgcaagcccg actgcgtttt cctgttccgc gatttcaacg
     2281 acgagccgca ttatcatgcg gtgctgcgcc atgtggaggt gctgacgcgc gtcgacgggc
     2341 tgggcgtgct acgcgcgaag cggcaggtcg acgggacggc ggtgctgcac gatctgttcg
     2401 accattatct cgaccccgat tgaccgctcc gccgcgcttc ggcacacccg cgcccatggc
     2461 ttccgaaacc ttcagcgccc cgcccaccat cgaaaccgac cgcctgcgcc tgcgccccgg
     2521 gcgcctcgcc gacaaggatg ttcacatcac catgtgggcc gacgagcgcg tcacgcgctt
     2581 catcggcggc gaaccccgcg cgcccgacgt cagctggggc aagttcctga gctcggcggg
     2641 gctgtggccg gtgatgggat tcggttactg ggtgttcgcc gaccgcgcga gcgacgcgct
     2701 gatcggcatg ggggggctca gctatttctg ccgcggcatc cccgagctcg aagggcggcc
     2761 cgaggcgggc tgggcgttcg atgccgacca ttggggcgcg ggctatgcga ccgaggcgat
     2821 gaccgcggcg ctgaactggg ccgatgcgaa tctcgatgcg accgacgtgc gctgcatcat
     2881 cgacctcggc aacgacgcgt cggagcgtgt ggcggcgaag ctggggttcc ggcgcatcgg
     2941 cgagagcgac gcgctcgggc atgtcgtcgc gatctattcg cggccgaggg gcggttaggc
     3001 acgacctttt cgccctctcg ttgcgcgccc aaggaggaac cttccgtcgc ggccgcgcgt
     3061 attcggccag agttcgaccg ggaggaatat catgcgatcg aagatgttta cgctggcggg
     3121 cgcggctgcg ctggcgctgg gagcctgcac ggtccaggag gacaaggaca aggcggccgg
     3181 cccggaggtc gccgaaagcg gcggcgtcgc gcgcgaagcg atgaaggccg aggagccgga
     3241 ggggccgccg gtgacgccga tgcccgccgc caccgtgatc cagtcgcagc cgggccccga
     3301 cggatcgcaa gtcgacctgc tctcggtcaa ggtcaccggc gacatattga ccgtcaccct
     3361 gcgctgttcg agccaggaga agcacaatcg cgaatcgctg cgcgtcgccc agatcagcgt
     3421 gatcgacgat gccacgtcgc agcgcatcgg cgtgctcaag gacaatgagg gtggcgcgat
     3481 ggtgtcgaac ctcaaccgca gcagcaattc ggactatgac acgatgggcg tcgactgcac
     3541 gtccaagccc ggtgtgatgt gggccaaatt cgcggcgccg ccggcgacca gcaagacggt
     3601 ttcgatcaac tttcccaata tcgcaccgtt cgatggcata gccgtccagc gatgaggcgg
     3661 gcgatagcct cgctgggtac ggtggcgctg cttgccggct gttcgggcaa tgcgcccgag
     3721 aatgtggccg gcgagggcga aaggcaggcg gcaccgacgg cggtcgtcag cgaaaccgac
     3781 cctacgcccc ctgcgccggg gttgcgcgcg gaggtcagcc cgctgtcggg caagctcagc
     3841 ggcctcaata cccgcgttac cgacatgggc acgatcatcg acctgccgtc cgatgccctg
     3901 ttcgaatatg acaaggcggc gctgaccccc gccgccgagg tggaattgcg caaggcggcc
     3961 gaactgatcc gcgcgagccc gtccggcgcg atcgagatca tcggtcatac cgacagcaag
     4021 ggcgacgaca gctataatct gacgctgtcc gaagcgcggg caaggacggt cgccgactgg
     4081 ttcggccgtc aggccggcgt ccgtcagcgc gaatttcgcg tgtcgggcaa gggcgaaagc
     4141 gccccgatcg tgccgaacga ggatgcgggc ggtgccgacg atccggcggg acgcgcgaag
     4201 aaccggcggg tcgaggtggt ggtgccgacc gcggcgggct gaccctgacc
//
LOCUS       DATMSU010000435         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_65032, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000435 DATMSU010000000
VERSION     DATMSU010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65032"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..2221)
                     /locus_tag="VN034_11655"
     CDS             complement(95..2221)
                     /locus_tag="VN034_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61305.1"
                     /translation="MRSKLKTGSGLGVVAALLWAAPAAAQTADEADGAADANEIVVTA
                     QKREERLQDVPISITSLGGKDLDAQASGGVSEALRSVAGISITNSPQGGGTQLTIRGV
                     AANGATLAGSSTSAFYIDSVPFGLVKSAILPDTNAYDMSRIEVLRGPQGTLYGANALN
                     GVVRVLTNDANLDRFELKTRAGVAATEGGDQSYRGDMAVNIPLVEGKLGLRVVGGFES
                     AGGWIDQPARGIKNANDSRSRNVRIKLNGQPTDDFSFGLSAWISRIDQDAPAYANDDN
                     NQLTTVPLPQKLDYDAFSAKLGYDFGTFEVSSATSYLKFSNLSERDYTNLGANQTLHT
                     EISSKIFTEELLINSTGGGDWRWSVGGFYRDASDLLNQTLFVLPAPINFKDKSRSFAA
                     FGEVTRSFMDGTFEVTGGLRYFKDRVTQIELTPTTGNPGQPLARRTDTFDAVTPRAVV
                     TWLPSRRFTAYASYSQGFRSGFNQSPTIIRTAPGIPPVKADRLSNYEVGAKGSLLGGA
                     LTFDAAVYYIDWKDIQQNLNVDIDGVVFAASVNGPKASGFGADLALTAHPIQGLDIGG
                     TFSWNGLDLSGPIVTQTAGGPVTLYQKGDRLAFSPEYTASGFLDYRFPVGQAGLKGIL
                     SGSLTYRSKIPARLLVGGAARYFLSDEPLLARASLGLESPDNWTASLYVENIGNWNGI
                     TQPPTDISQEYRVRPRTVGLQFEFRY"
     gene            2382..2816
                     /locus_tag="VN034_11660"
     CDS             2382..2816
                     /locus_tag="VN034_11660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWV61306.1"
                     /translation="MTSTPVETFRGVVFPWHCDAMGHMSVQHQMPLLDNAVYHLLGFF
                     GPTAGEVDGRNAGWADVSHEIRYLNELVAGDLLVLSSGILSVGRSSIRHRTILSRRPD
                     ATACTILEGVTVRFDLDARKATPLSDRERAIAGQLMLPAETN"
     gene            2834..3592
                     /locus_tag="VN034_11665"
     CDS             2834..3592
                     /locus_tag="VN034_11665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61307.1"
                     /translation="MERVLVTGAGDSVGRAIAERFLQKGAKVHICDVRGDAVAATLAA
                     NPGMSGSVTDVGVRAEVDALFDDVRAHVGDLSILVNVVGMAGPRAPIEDIDEDEWDKT
                     IAVNLSGMFYTMKRAVPMLKANGGGSIVNFSTCSTRTGIPFRAPYIASKAGVEGLTKN
                     SARELGPSGIRVNAILPGIINNDRFRFIVQRNVDATGRSFEEIENDYLKYVSLRCKVE
                     LSEFADMVEFITSDAGKKITGEIIAVSGNMEWEE"
     gene            3589..4494
                     /locus_tag="VN034_11670"
     CDS             3589..4494
                     /locus_tag="VN034_11670"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HWV61308.1"
                     /translation="MTMNKKTILTCAVTGGAPYNKKHPSMPVTPKQIADACIEAASAG
                     ASIVHIHVRDPETGEGNRDRAHFREVVDRVRQTGTDIVLNLTCGMGAYFLPDPENEGH
                     MLPGSDVVGVDERVAHIEELLPEIATLDIVTNNQVEGPTEYIYFSPTHTLRAMAKRFQ
                     AAGVKPELEVFGPGDILFGNTLVEEGLIDGPAMMQMVLGVQWAAPHGVETILYQKSLM
                     APGTIWGAFGIGREQMPMLAHTLLLGGNIRVGLEDNLYMSRGVWATNGQLVERAYTLV
                     NAVGGYDVATPAETREILKLRTPGA"
BASE COUNT          806 a         1372 c         1591 g          823 t
ORIGIN      
        1 atgggtggcg ggggagggga gatcgctatc gggggagggc acgggcaaac cttttgcaaa
       61 cgggggccgg atcgctccgg cccccacaag agactcaata acggaattcg aactgcaggc
      121 ccacggtgcg cggacgcacg cgatattcct ggctgatgtc cgtcggcggc tgggtgatgc
      181 cgttccagtt gccgatattt tcgacataga gcgaggcggt ccagttgtcg ggcgattcga
      241 gcccgaggct ggcgcgcgcg agcagcggtt cgtcgctgag gaaatagcgc gcggcgcccc
      301 cgacgagcag gcgtgcgggt atcttcgagc gataggtcag cgatcccgac aatatgccct
      361 tcaatcccgc ttggccgacc gggaagcgat agtcgaggaa gccgctcgcc gtatattcgg
      421 gcgagaaggc gagccggtcg cccttttgat agagggtgac ggggccgccc gcggtctgcg
      481 tgacgatcgg gcccgacagg tcgaggccgt tccagctgaa ggtgccgccg atgtcgagcc
      541 cttggatagg gtgcgcggtc agcgcgaggt cggcgccgaa gccgctcgcc ttgggcccgt
      601 tgaccgatgc ggcgaagacg acgccgtcga tatcgacgtt cagattctgc tgaatatcct
      661 tccagtcgat ataatagact gcggcatcga aggtgagcgc gccgcccagg aggctgccct
      721 tggcgccgac ctcatagttc gacaggcggt cggccttgac cggcgggatg ccgggggcgg
      781 tgcggatgat cgtcgggctc tggttgaagc cgctgcggaa accctgcgag taggaggcat
      841 aggcggtgaa cctgcggctc ggcagccagg tgaccaccgc gcgcggggtt accgcgtcga
      901 aggtgtcggt gcggcgcgcg agcggctgtc ccgggttgcc cgtggtgggg gtcagctcga
      961 tctgggtcac gcgatccttg aaatagcgca gcccgccggt gacttcgaac gtgccgtcca
     1021 tgaagctgcg cgtgacttcg ccgaacgccg cgaacgagcg cgacttgtcc ttgaagttga
     1081 tcggtgcggg gaggacgaac aaggtctggt tcaaaaggtc ggacgcgtcg cggtagaagc
     1141 cgccgaccga ccagcgccag tcgccgccgc cggtcgaatt gatcagcagc tcttcggtga
     1201 agatcttcga gctgatttcg gtgtgaagcg tctggttggc gccgaggttg gtatagtcgc
     1261 gctcgctgag gttggagaat ttgaggtagc tggtcgcgct cgatacctcg aacgtgccga
     1321 agtcgtagcc gagcttggcg ctgaacgcgt catagtcgag cttttgcggc agcggcacgg
     1381 tggtgagctg gttattgtcg tcgttggcat aggcgggcgc gtcctgatcg atgcgcgaaa
     1441 tccatgccga caggccgaag ctgaagtcgt cggtcggctg gccgttgagc ttgatacgca
     1501 cgttgcgcga gcggctgtcg ttggcgttct tgatgccgcg cgccggctga tcgatccagc
     1561 cgccggcgct ttcgaagccg ccgacgacgc ggaggccgag cttgccttcg acgagcggaa
     1621 tgttgaccgc catgtcgccg cgatagctct ggtcgccgcc ctcggtcgcg gcgacgccgg
     1681 cgcgggtctt gagttcgaag cggtcgaggt tggcgtcgtt ggtgaggacg cggacgacgc
     1741 cgttcagcgc gttggcgccg tagagcgtgc cttgcggccc gcgcagcact tcgatgcgcg
     1801 acatgtcata ggcgttggtg tcgggcagga tcgccgactt gacgaggccg aagggcaccg
     1861 aatcgatata gaaggccgag gtgctcgacc cggcgagcgt cgcgccgttc gcggcgacgc
     1921 cgcggatcgt gagctgggtc ccgccgccct ggggcgagtt ggtgatcgag atgccggcga
     1981 ccgagcgcag cgcttcggag acaccgccgc tggcctgcgc gtcgaggtcc ttgccgccga
     2041 gcgaagtgat cgagatcggc acgtcctgga gtcgttcttc gcgtttttgc gcggtcacca
     2101 cgatttcgtt cgcatccgcg gcgccgtcgg cttcgtccgc cgtctgcgcg gcggcaggcg
     2161 ccgcccacag cagtgccgcg acaacgccaa ggcccgatcc agtcttcagt ttgctacgca
     2221 taatgtcccc ttttgcacgt tttatgaatt gtgatgagag tgcgaatacc caatgaacac
     2281 attgtgtata atctgtgtag acaagtcaag cgcgggcgat tagtccgggg ccatcggcaa
     2341 cggagaggcg cgtcatggaa ccggaaaaca ccttggaatc agtgacaagc accccggtcg
     2401 aaacctttcg cggagtcgtc tttccctggc actgtgatgc gatggggcac atgtcggtgc
     2461 agcatcagat gccgctgctc gacaatgcgg tttatcattt gctcggcttt ttcggcccca
     2521 ccgcgggcga ggtcgatggc cgcaacgcgg gctgggccga cgtctcgcac gagattcgct
     2581 atctcaacga actggtcgcg ggcgacctgc tcgtcctttc gagcggcata ttgtcggtcg
     2641 gccgctcgtc gatccggcac cggacgatat tgtcgcggcg ccccgatgcg acggcctgca
     2701 cgatattgga aggcgtcacc gtccgcttcg acctggacgc gcgcaaggcg acgcccttgt
     2761 ccgaccgcga acgcgccatt gccgggcagc tgatgctgcc ggccgaaacg aactgaattt
     2821 agagggaggc ttcatggaac gagtgctggt tacgggtgcg ggcgacagcg tcggccgcgc
     2881 gatcgccgag cggtttctgc aaaagggcgc gaaggtgcat atttgcgacg tgcgcggcga
     2941 cgcggtcgcg gcgacgctgg cggctaatcc ggggatgagt ggcagcgtca ccgatgtcgg
     3001 cgtgcgcgcc gaggtcgacg cgctgttcga tgacgtgcgc gcgcacgtcg gcgacctttc
     3061 gatcctggtc aatgtcgtcg gcatggcggg gccgcgcgct ccgatcgagg atatcgacga
     3121 ggatgagtgg gacaagacga tcgcggtcaa cctgtcgggc atgttctata cgatgaagcg
     3181 cgccgtcccg atgcttaagg ccaacggtgg cggatcgatc gtcaattttt cgacctgttc
     3241 gacgcgcacc ggcatccctt ttcgcgcgcc ctatatcgcg tcgaaggcgg gggtcgaggg
     3301 gctgacgaag aacagcgcgc gcgaactggg cccgtcgggc atccgcgtca acgcgatcct
     3361 gcccggcatc atcaacaacg accgctttcg cttcatcgtc cagcgcaacg tcgacgcgac
     3421 gggccgcagc ttcgaggaga tcgagaacga ctatctcaaa tatgtgtcgc tgcgctgcaa
     3481 ggtcgaactc tccgagttcg cggacatggt cgaattcatc acctcggacg ccggcaagaa
     3541 gatcaccggc gagatcatcg ccgtcagcgg caacatggaa tgggaagaat gacgatgaac
     3601 aagaagacga tcctgacctg cgcggtcacc ggcggcgcgc cctataacaa gaagcatccg
     3661 tcgatgccgg tcacgccgaa gcagatcgcc gacgcctgta tcgaagccgc ttcggcgggt
     3721 gcgagcatcg tccacatcca cgtccgcgac cccgagacgg gcgagggcaa ccgcgaccgt
     3781 gcgcatttcc gcgaagtcgt cgaccgcgtg cgccagacgg gtaccgacat cgtgctcaac
     3841 ctcacctgcg gcatgggcgc ctatttcctg cccgaccccg aaaatgaggg gcatatgctg
     3901 ccgggcagcg acgtcgtcgg cgtcgacgag cgcgtcgcgc atatcgagga actgctcccc
     3961 gagatcgcga cgctcgacat cgtgaccaac aaccaggtag aagggccgac cgaatatatc
     4021 tatttcagcc cgacgcacac gctgcgcgcg atggcgaaac ggttccaggc ggcgggggtg
     4081 aagcccgagc tcgaagtgtt cggcccgggc gatatattgt tcggcaacac gctggtcgag
     4141 gaagggctga tcgacggccc ggcgatgatg cagatggtgc tcggcgtgca atgggcggcg
     4201 ccgcacgggg tcgagacgat cctgtatcag aagagcctga tggcgcctgg cacgatctgg
     4261 ggcgcattcg gtatcgggcg tgagcagatg ccgatgctcg cgcacacatt gctgctcggc
     4321 ggcaacatcc gcgtcggtct ggaggacaat ctttatatgt cgcgcggcgt atgggcgacc
     4381 aacggccagc tcgtcgagcg cgcctatacg ctcgtcaacg cggtcggcgg ctatgacgtc
     4441 gcgacgcccg ccgagacgcg cgagatattg aagctgcgaa ccccgggcgc atgaccgaag
     4501 ccgccgcgac ggttgcttat tacgacgcgg cggcggtcgc gcgggcaacg ccgatgcccg
     4561 cgctgatcgc cgcgttgcgg cgcgccttcg ct
//
LOCUS       DATMSU010000436         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_73867, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000436 DATMSU010000000
VERSION     DATMSU010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73867"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VN034_11675"
     CDS             <1..853
                     /locus_tag="VN034_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWV61309.1"
                     /translation="YLFSGPTGVGKTEVAKQLASIMGIPLQRFDMSEYMERHSVSRLI
                     GAPPGYVGYDQGGLLTDAIDQNPHCVLLLDEIEKAHPDLFNILLQVMDNGRLTDHHGK
                     TVDFRNVILIMTTNAGASDMARESIGFGQSTREDVQEEAVKRMFTPEFRNRLDAIVPF
                     GYLPPEVVARVVDKFILELELQLADRNVHIQLDDAAREWLTGKGYDKLYGARPMGRLI
                     QEKIKQPLAEELLFGKLVHGGEVKVKMKTGEDAHVGNPLAFEITPAAPKAGKGKAKAD
                     KAKKAAE"
     gene            895..1122
                     /locus_tag="VN034_11680"
     CDS             895..1122
                     /locus_tag="VN034_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008072067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWV61310.1"
                     /translation="MNTPLKITKVGNSAAVILPKELLARLRVSLGDTLYATESPDGIR
                     LTAADPDFEEKMAAAEAIMREDRDILRVLAK"
     gene            1119..1517
                     /locus_tag="VN034_11685"
     CDS             1119..1517
                     /locus_tag="VN034_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HWV61311.1"
                     /translation="MTDDARAEPRWLGIDVALAVHDRQLAEHGGIAGVRDPGMLESAL
                     ARPVNQWAYGEDDPAKLAAAFAFGIARNHPFGDGNKRTAWVLARLFLAINGHDLRFEA
                     QYAIDTMLALAAGTLSEAALADWFRDRLAF"
     gene            1543..2382
                     /gene="map"
                     /locus_tag="VN034_11690"
     CDS             1543..2382
                     /gene="map"
                     /locus_tag="VN034_11690"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWV61312.1"
                     /translation="MYDYVSVTADEASAPARVRDGTIKLHDEAGFAGMRKAGRLSAEI
                     LDALVPFVQPGVTTAEIDNLVRTMMLDGGGIPATLGYRGFTHSCCTSINHVVCHGIPD
                     DKPVREGDIVNIDVTSIVDGWHGDTSRMYLVGDVPIKAKRLVEVTYECLMLGIEQAKP
                     GNRMGDVAHAIQQHAERHRYSVVRDFCGHGLGQMFHDAPEVVHAGRPGTGPELKPGMF
                     FTIEPMINTGKYAVKMLSDGWTAVTRDRSLSAQFEHSIGITESGCEIFTASPKGLNAP
                     PWQ"
     gene            2746..>4580
                     /locus_tag="VN034_11695"
     CDS             2746..>4580
                     /locus_tag="VN034_11695"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HWV61313.1"
                     /translation="MAKRVFSAALLGCAALALASCNASKNESISGGALDAAEKASETL
                     LKTGGDGDDWGAIGYSYDEQRFSPLTDINDQNVGTLGIAWFADLDDARGQEATPVVVD
                     GVMYVTHAWSKVSAWDAATGKPLWKFDPEVPGERAVSACCDVVNRGVAVWGDKVFVGA
                     LDGRLIALDRKTGKKLWSTQTFDKRRPYTITGAPRVVKDMVLIGNGGAEFGVRGYVSA
                     YDADTGKLRWRFYTAPNPEKKKDGAASDDIFASKGNTTWSDAGEWQTSGGGGTVWDAI
                     VYDKDLDQIYLGVGNGNPWNHGTRSNGEGDNWFLSSVVALDAATGKYKWHYQETPAET
                     WDYTATQPIILAEQQVGGKPVKVLYHAPKNGFFFTIDRMSGKLIDAKPFVDGINWATG
                     YDLATGRPIENPEARFYKTGKPFIAIPGALGAHNWHPMSYNPATGLVYIPAQQIPQGY
                     LADMNELDRRKVVGFNVGTSLTGTMMPDDKAAFRAAVAATTGRLVAFDPRTGKVAWAV
                     DYPAAWNGGTMSTAGNLVFQGTSTGRFRAYAADTGRQLLDLDMQSGIVSAPSTFRVGG
                     VQYVAFQTSKGGAFPLVAGVAGGVTRKVPNIPRLVVLKLGGTVQLP"
BASE COUNT          871 a         1507 c         1421 g          781 t
ORIGIN      
        1 ctatctgttc agcggcccga ccggcgtcgg caagaccgag gtcgcgaagc agctcgcgtc
       61 gatcatgggc atcccgctcc agcgcttcga catgtccgaa tatatggagc gccattcggt
      121 cagccgcctg atcggcgcgc ctccgggcta tgtcggttat gatcagggcg gtctcctgac
      181 cgatgcgatc gaccagaacc cgcattgcgt gctgctgctc gacgagatcg aaaaggcgca
      241 tcccgacctg ttcaacatcc tgttgcaggt gatggacaac ggccgcctga ccgaccacca
      301 cggcaagacc gtcgatttcc gcaacgtcat cctgatcatg acgaccaacg cgggcgcgag
      361 cgacatggcg cgcgaatcga tcggtttcgg ccagtcgacg cgcgaggatg tgcaggaaga
      421 ggcggtgaag cgcatgttca cccctgaatt ccgcaaccgc ctcgatgcga tcgtgccctt
      481 cggttacctg ccgcccgagg tcgtcgcgcg cgtcgtcgac aagttcatcc tcgaactcga
      541 actccagctc gccgaccgca acgtccacat ccagctcgac gacgcggcgc gcgaatggct
      601 gaccggcaag ggctatgaca agctctatgg cgcgcgtccg atgggccgcc tgatccagga
      661 aaagatcaag cagccgctcg ccgaggaact gctcttcggc aagctggtcc atggcggcga
      721 ggtcaaggtg aagatgaaga ccggcgagga cgcccatgtc ggcaatccct tggccttcga
      781 aatcaccccc gccgcgccca aggccggcaa gggcaaggca aaggccgaca aggcgaagaa
      841 ggcggcggag taagcctggt tgaaattctt gagcggcggc gttataacga acgtatgaac
      901 acgccgctca agatcaccaa ggtcggcaac agcgccgccg tcatcctgcc caaggaattg
      961 ttggcgcggc tacgtgtgtc gttgggcgac acgctctatg cgacggaatc gcctgatgga
     1021 attcgcctga cggctgccga ccccgacttt gaagagaaaa tggccgcagc cgaagcgatt
     1081 atgcgggaag accgcgatat tctccgcgtt cttgccaagt gaccgacgac gctagggccg
     1141 agccgcgatg gctcggcatc gatgttgcgc tggccgttca cgaccgacag ctcgcagaac
     1201 atggcggcat agccggggtc cgcgatcccg gcatgctgga atcagcattg gcaagacccg
     1261 tcaatcagtg ggcgtatggc gaagacgatc cggcgaaact ggccgctgcc ttcgctttcg
     1321 gcatcgcccg aaatcatccg tttggcgatg gcaacaagcg gacggcatgg gttcttgctc
     1381 gacttttcct cgcgatcaat ggtcacgacc tccgtttcga ggcgcaatat gccatcgata
     1441 ctatgcttgc actcgctgcc ggcacactct ccgaggcagc cttggctgat tggtttcggg
     1501 accgtctcgc tttttgaggc cccatcccct atatcccctc ccatgtacga ctatgtctcc
     1561 gtaaccgccg acgaagcctc ggcccccgcc cgcgttcgcg acggcacgat caagcttcat
     1621 gacgaagcgg gctttgccgg gatgcgcaag gccggccgat tgtcggccga aatcctcgac
     1681 gcgctcgttc ccttcgtcca gccgggggtg accaccgcgg aaatcgataa cctcgtccgc
     1741 acgatgatgc tcgatggcgg cggcatcccc gcgacattgg gctatcgcgg cttcacgcac
     1801 agctgctgca ccagcatcaa ccatgtcgtc tgccatggta tccccgacga caagccggtg
     1861 cgcgagggcg acatcgtcaa catcgacgtc accagcatcg tcgacggctg gcacggcgac
     1921 acgagccgca tgtatctggt cggcgatgta ccgatcaagg cgaagcgcct cgtcgaggtc
     1981 acctatgaat gcctgatgct cggcatcgag caggcgaagc ccggcaaccg tatgggcgac
     2041 gtcgcgcacg cgatccagca acatgccgag cgccaccgct attcggtcgt ccgcgatttc
     2101 tgcgggcacg gtctcggaca gatgttccac gacgcgcccg aagtggtcca cgccggtcgc
     2161 cccggcaccg gccccgaact caagcccggc atgttcttca cgatcgagcc gatgatcaac
     2221 accggcaaat atgcggtgaa gatgctgagt gacggctgga ccgcggtgac ccgcgaccgc
     2281 agcctctcgg cgcagttcga gcacagcatc gggatcaccg aaagtggctg cgagattttc
     2341 accgcgagtc cgaagggtct gaacgcgccg ccgtggcagt gagtttatag aattggcgag
     2401 aagggctgag ggcgctggga tttggttcac gcggagacgc ggagccgcgg agaaatagcc
     2461 cgccttcgtt cgtgctgagc ttgtcgaagc actgctcttc tttttgacgc cgcaagaaga
     2521 aagaacggcc cttcgacgag ctcagggcaa acggtatcgg ggctcgcgac tatctccgcg
     2581 tctccgcgtg aacacctcct tccttctctg cgatctctgc gcctctgcgc gaattccttc
     2641 ttcccgctcc cgctccaccc cccattttcc cgcttgcgcc cttgcgcctt cgcgcgcagt
     2701 cttgacgtta acgtcaagca taggcgtcga acgggagagc aagggatggc gaaacgggtc
     2761 ttttcggccg cgctgctggg ctgcgcggca ctggcattgg cttcgtgcaa tgcatcgaag
     2821 aatgagagca tctcgggcgg cgcgctcgac gcggccgaaa aagccagcga gaccctgctc
     2881 aagaccggcg gcgacggcga cgactggggc gccatcggct acagctatga cgaacagcgt
     2941 ttcagcccgc tgaccgacat caacgaccag aatgtcggca ccctcggcat cgcctggttc
     3001 gccgatctcg acgatgcgcg cgggcaggaa gcgacgcccg tcgtcgtcga cggcgtgatg
     3061 tatgtcaccc acgcctggtc gaaagtcagc gcgtgggacg ccgcgacggg caaacccttg
     3121 tggaaattcg accccgaagt gcccggcgag cgcgcggttt cggcctgctg cgacgtcgtc
     3181 aaccgcggcg tcgccgtctg gggcgataaa gtcttcgtcg gcgcgctcga cggccggctg
     3241 atcgcgctcg accgcaagac cggcaaaaag ctgtggtcga cgcagacctt cgacaagcgc
     3301 aggccctata cgatcaccgg cgcgccgcgg gtggtgaagg acatggtgct gatcggcaac
     3361 ggcggcgccg aattcggggt gcgcggctat gtcagtgcct atgacgccga caccggcaag
     3421 ctgcgctggc gcttctacac cgcgccgaac cccgagaaga agaaggacgg cgcggcgtcg
     3481 gacgacatct tcgcgtcgaa gggcaacacg acctggtcgg acgcgggcga atggcagacc
     3541 tcgggcggcg gcggcaccgt ctgggacgcg atcgtctacg acaaggatct cgaccagatc
     3601 tatctcggcg tcggcaacgg caacccgtgg aaccatggca cgcgctcgaa cggcgagggc
     3661 gacaactggt tcctctcctc ggtcgtcgcg ctcgatgccg cgaccggcaa atataaatgg
     3721 cactatcagg agacgccggc cgagacgtgg gactataccg cgacccagcc gatcatcctc
     3781 gccgagcagc aggtgggcgg caaacccgtc aaggtgctgt accacgcgcc gaagaacggt
     3841 ttcttcttca ccatcgaccg catgagcggc aagctgatcg atgccaagcc cttcgtcgac
     3901 gggatcaact gggcgaccgg ctatgacctt gcgaccggcc gcccgatcga aaatcccgag
     3961 gcgcgcttct acaagaccgg caagcctttc atcgcgattc ccggcgcact cggcgcgcac
     4021 aactggcatc cgatgagcta caatcccgcg accggcctcg tctacatccc ggcgcagcag
     4081 atcccgcagg gctatctcgc cgacatgaac gagctcgacc ggcgcaaggt cgtcggcttc
     4141 aacgtcggca cctcgctcac cggcacgatg atgcccgacg acaaggcggc gttccgcgcc
     4201 gcggttgccg ccacgaccgg gcgcctcgtc gccttcgatc cgcgcaccgg caaggtcgcg
     4261 tgggcggtcg attaccccgc ggcgtggaac ggcggcacga tgtcgaccgc gggcaatctc
     4321 gtctttcagg gcacgagcac cggccgcttc cgcgcctatg ccgccgacac cggcaggcag
     4381 ttgctcgatc tcgacatgca gtcggggatc gtcagcgcac cctcgacctt ccgtgtcggc
     4441 ggcgtccaat atgtcgcctt ccagacgagc aagggcggcg cctttccgct cgtcgccggg
     4501 gtcgcgggcg gtgtgacgcg caaggtgccc aatattccgc gcctcgtggt gctcaagctc
     4561 ggcggtacgg tgcagctccc
//
LOCUS       DATMSU010000437         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_7929, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000437 DATMSU010000000
VERSION     DATMSU010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7929"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VN034_11700"
     CDS             complement(<1..465)
                     /locus_tag="VN034_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWV61314.1"
                     /translation="MTAESGENSAANRVRMGRPGAAGAGRDRFGLTGTSQDFDPRVVA
                     IRPDLADIAAAGTHFAPHYAAPMMRSGVLPAAALRASPSVDADQTSELLFGEGFALLD
                     LTGGWAWGYCLADHYVGYLAAEALAAPIAPTHRVEMIEAMLHSAPDAASGGPA"
     gene            complement(551..1954)
                     /locus_tag="VN034_11705"
     CDS             complement(551..1954)
                     /locus_tag="VN034_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HWV61315.1"
                     /translation="MTDYSDLIQPDKGQDARTIHLVDKKGYDAWLKGRSARERAHLAA
                     VGFKPDAFVHAILPGDDPEKWAVVTTVAEVDSLSAWCLAKLGQILPEGRYRLEGQQPG
                     KALFGWMSAQYRFDAYKSNAPVKGPRVLLTSDVGAIAPLVAEMRAVALVRDLVNTPAA
                     DMGPAAIEKAAERIAKAHGGKLTVTKGEALEQGYPMIHAVGRAAAKHHVPRLIEIEWG
                     KEGHPRIALVGKGISFDSGGLDIKPAAGMRLMKKDMGGAAHVLALAELVMASGLPVRL
                     HCLVAAAENAISSDAFRPGDVLKSRLGLTVEIGNTDAEGRLVLGDALAKAGEEKPELI
                     VDFATLTGAARVALGPDLPPLYANDDALADAMLAGGIERDDPLWRMPLWDGYADLLET
                     DIADLGNAGSSPFAGSITAALFLKRFVPEGTLWAHLDTFAWRPSAKPGRPKGGAALGL
                     RAAWAMLQARYDRRVKS"
     gene            2010..2837
                     /locus_tag="VN034_11710"
     CDS             2010..2837
                     /locus_tag="VN034_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HWV61316.1"
                     /translation="MMIDPHWRNAIPILAGALLLAGCSEDKPTKAEKVAAASVPGAPP
                     EGAADEAAKQAPASNVSENNELIEFAYSYPADAARIPELAKALDDDRAAKREALVAAA
                     QRDKDAAEKEGFPYRKHSHLQTWKRVTSTPRFLSLSTEIGTYAGGAHGMQTFDTLIWD
                     RNQRKRMKPVDLFESSAAFDAAIRDAFCAGIKRAKAAKGIEEAPDSVFAECPPASAQT
                     VWLGSSDGRYLDRLTIAIAPYEIGAYAEGSYKINVPMTGALVKVVNDEFARDFLPIN"
     gene            2850..3785
                     /locus_tag="VN034_11715"
     CDS             2850..3785
                     /locus_tag="VN034_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61317.1"
                     /translation="MAKQPKAGKVGESAKEKKGKRSAAAKLRREVGGGKTIAKPPSDR
                     EADLDRAPKWQPRYPGSGRLEGKVAIVTGGDSGIGRAVCALFAREGADIAVVYLENRA
                     DAVETAAIVEAEGRRAIAIKADVGKPKAGEKIVAQTVEKLGRLDILVNNAGEQHPAAD
                     IRDISEDQLQQTFATNIFGMFYLVQAALPHLKKGAAIVNCTSVTMYQGSKGLLDYSAT
                     KGAITAFTRSLSENLIEKGIRVNGVAPGPIWTPLNPRGGAPAEKVATFGESTPMKRPG
                     EPNEVAPCFLFLACDDSSYMSGQVLHPNGGTIVNG"
     gene            3952..4134
                     /locus_tag="VN034_11720"
     CDS             3952..4134
                     /locus_tag="VN034_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HWV61318.1"
                     /translation="MPAKSKAQQKAAGAALSAKRGDTPKSKLKGASKQMVDSMTEKQL
                     EDFAKGSTKGKPEHAD"
     gene            complement(4138..>4867)
                     /locus_tag="VN034_11725"
     CDS             complement(4138..>4867)
                     /locus_tag="VN034_11725"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWV61319.1"
                     /translation="PEKVKEFLTKKLKASLGDHYDAEAFTPPYNPWEQRLCLVPDADF
                     FEAMKAGEASVVTDHIEKFDAAGIQLKSGKHLDADIIITATGLKLAVAGKIPVRVDGT
                     PVDWHDHYYYKACMFSNIPNFSAVFGYLNASWTLRADVVSEYVCRVLNHMRDTGTTVA
                     TPALADPSSLEEENVFDFSSGYIQRSLHIMPKSTAALPWRLSQNYVQDRVDMRTGAIA
                     DGVLTFSNAKAKVAAAPAELEAAE"
BASE COUNT          897 a         1595 c         1611 g          764 t
ORIGIN      
        1 ggcggggccg ccgctcgcgg catcgggggc gctatggagc atcgcttcga tcatttcgac
       61 gcggtgcgtc ggcgcgatcg gcgcggcgag ggcttcggcg gcgaggtagc cgacataatg
      121 atcggcgagg caatagcccc acgcccagcc gccggtgagg tcgagcaggg caaagccctc
      181 gccgaacagc aattcgctcg tctggtcggc atcgaccgac ggcgatgcgc gcagcgccgc
      241 ggccgggagg acgccgctgc gcatcatcgg cgcggcgtaa tgcggcgcga aatgcgtgcc
      301 ggcggcggca atatcggcca ggtcgggacg gatcgcgacg acgcgcggat cgaaatcctg
      361 cgacgttccg gtcaaaccga accggtcacg gcctgcgccc gcagcgccgg gacgtcccat
      421 ccgcacgcga ttggccgctg aattttcgcc gctctctgct gtcacttgcc gtcctgttga
      481 aacccgaggt ccgccgggcg cggacaatcg cgggccatta gaccagcagg gcctgcctta
      541 tcaagaaagt tcagcttttg acgcgccggt cgtagcgcgc ctggagcatc gcccacgccg
      601 ctcgcaatcc gagcgccgcg ccgcccttgg gccgaccggg cttggccgag ggacgccagg
      661 cgaaggtatc gagatgcgcc caaagggtgc cttcggggac gaagcgtttg aggaacagcg
      721 ccgcggtgat cgagccggcg aaaggcgaac tgcccgcatt gccgaggtcg gcgatgtcgg
      781 tttcgagcag gtcggcatag ccgtcccaga gcggcatccg ccacaaggga tcgtcgcgct
      841 cgatcccgcc cgcgagcatc gcatcggcga gcgcgtcatc attggcatag agcggcggca
      901 gatcgggccc gagtgcgacg cgcgccgcgc cggtcagtgt cgcgaaatcg acgatcagct
      961 cgggcttttc ctcgcccgcc tttgcaagcg cgtcgccgag gacgagccgg ccttcggcat
     1021 cggtgttgcc gatctcgacc gtcagcccga ggcggctctt cagcacatct ccggggcgga
     1081 aggcgtcgga cgagatcgca ttttcggccg cggcaacgag acagtggagg cgcaccggca
     1141 gcccgctcgc catcaccagt tcggcgagcg cgagcacatg cgcggcgccg cccatatcct
     1201 tcttcatcag ccgcattccc gccgcgggct tgatgtcgag cccgccgctg tcgaagctga
     1261 tccccttgcc gacgagcgcg atgcgcggat gaccctcctt gccccactcg atctcgatca
     1321 gccgcggcac atgatgcttc gccgcggcgc gcccgaccgc gtggatcatc ggataacctt
     1381 gttcgagcgc ctcgcccttg gtgaccgtca gtttgccgcc atgcgccttg gcgatgcgct
     1441 cggccgcctt ctcgatcgcc gcaggcccca tatcggcggc gggcgtgttg acgaggtcgc
     1501 ggacgagcgc gaccgcgcgc atctcggcga ccagcggcgc gatcgcgccg acgtcgctgg
     1561 tcaaaaggac gcgcgggcct ttgacgggcg cgttcgattt ataggcgtcg aagcgatatt
     1621 gcgcgctcat ccagccgaac agcgccttgc cgggctgctg gccttcgagc cggtagcggc
     1681 cctcgggcag gatctgcccc agcttggcga ggcaccaggc cgacaggctg tcgacctcgg
     1741 caaccgtcgt gacgaccgcc catttttcgg gatcgtcgcc cggcaggatc gcgtggacga
     1801 acgcatcggg cttgaaaccc accgcggcga gatgcgcgcg ttcgcgcgcg ctgcggccct
     1861 tcagccacgc atcataaccc ttcttgtcga cgagatggat ggtgcgggca tcctgtccct
     1921 tgtcgggctg gatcaggtcg gaatagtcgg tcatgcgggc cgtgctaggc cgctcgggat
     1981 attttgtcga ttcccggcgt tatgtcagca tgatgatcga cccacactgg cgaaatgcca
     2041 tcccgatcct tgccggcgcg ctgttgctgg cgggttgttc cgaagacaag ccgaccaagg
     2101 cggaaaaagt cgccgcggcg tcggtgcccg gcgcgccgcc cgagggcgcc gccgatgaag
     2161 ccgcaaagca ggcgccggcg tcgaatgtca gcgaaaacaa cgaactgatc gaattcgcct
     2221 attcctatcc cgccgatgcc gcgcgaatcc ccgagctggc gaaagcgctc gacgacgatc
     2281 gcgcggcaaa gcgcgaggcg ctagtcgcgg cggcacagcg cgacaaggac gcggcggaga
     2341 aggaaggttt cccttatcgc aagcacagcc atctccagac gtggaagcgc gtcaccagca
     2401 ccccgcgctt cctgagcctg tcgacggaga tcgggaccta tgctggcggc gcgcacggca
     2461 tgcagacctt cgacacgctg atctgggacc gcaaccagcg caagcggatg aagccggtcg
     2521 acctgttcga gagcagcgcg gcgttcgatg ccgcgatccg cgacgccttc tgcgccggaa
     2581 tcaaacgcgc caaggcggca aaggggatcg aggaagcgcc cgacagcgtc ttcgccgaat
     2641 gcccgccggc gtcggcgcag acggtgtggc tcggctcgtc ggacgggcgc tatctcgacc
     2701 gcctgacgat cgcgatcgcg ccctatgaaa tcggcgccta tgccgaaggg agctacaaga
     2761 tcaacgtccc gatgaccggc gcgctcgtca aggtggtgaa cgacgagttc gcgcgcgact
     2821 tcctccccat caactgaaca ggacaagcca tggcgaagca gcccaaggcc ggcaaggtcg
     2881 gcgaaagcgc gaaggaaaag aagggcaaac ggagcgccgc ggccaagctg cgccgcgaag
     2941 tgggaggcgg caagacgatc gccaagccgc cgtccgaccg cgaagccgac ctcgatcgcg
     3001 cgccgaaatg gcaaccacgc tatccggggt cgggccgcct cgaaggcaag gtggcgatcg
     3061 tgacgggcgg cgacagcggt atcggccgcg cggtttgcgc gctgttcgcg cgcgagggcg
     3121 ccgacatcgc ggtcgtctat ctcgagaacc gggccgatgc cgtcgaaacc gccgcgatcg
     3181 tcgaggccga agggcggcgc gcgatcgcga tcaaggccga tgtcggcaag ccgaaagcgg
     3241 gcgaaaagat cgtcgcgcag acggtcgaaa agctcggccg cctcgacatc ctcgtcaaca
     3301 acgccggcga gcagcatccg gcggcggata tccgcgacat cagcgaggat cagctgcaac
     3361 agaccttcgc gaccaatatc ttcggcatgt tctatctggt gcaggcggcg ctgccgcacc
     3421 tcaagaaggg cgcggcgatc gtcaattgca ccagcgtgac gatgtatcag gggtcgaagg
     3481 ggctgctcga ttacagcgcc accaagggcg cgatcaccgc cttcacccgt tccttgagcg
     3541 aaaatctgat cgagaagggc attcgcgtga atggcgtcgc gcccggcccg atctggaccc
     3601 cgctcaaccc gcgcggcggc gcgccggccg aaaaggtcgc cacctttggc gagagcacgc
     3661 cgatgaaacg gccgggcgaa cccaatgagg tcgcgccctg cttcctgttc ctcgcgtgcg
     3721 acgacagcag ctatatgtcg ggccaggtgc tgcatcccaa cggcggcacg atcgttaatg
     3781 gctaggaccg atcgccattc aacccctggc ggcctgcaaa tggcggcttt ccgcgcttcc
     3841 ggtgctcacg tgcagaagca cgctgcgctc cgggtcacgg aaaaccacca ttttcgactc
     3901 accatggact aaatggcgat cggtcctagc ggggggcaaa ggagaaacgc catgccagcc
     3961 aaatcgaaag cccagcagaa agccgccggc gccgcactca gcgccaagcg cggcgacacg
     4021 cccaagtcga aactcaaggg cgcgtcgaaa cagatggtcg acagcatgac cgaaaagcag
     4081 ctcgaggatt tcgccaaggg ctcgaccaag ggcaagcccg agcacgccga ttagcgatta
     4141 ttcggcggct tcgagctccg cgggagcggc cgccaccttt gctttggcgt tgctaaacgt
     4201 cagcacgccg tcggcgatcg cgcccgtgcg catgtcgacg cggtcctgca cataattctg
     4261 gctgagccgc cacggcagcg cggccgtgct cttgggcatg atgtgcagcg atcgctggat
     4321 atagcccgag gagaagtcga agacattttc ctcttcgagg ctcgacggat cggcgagcgc
     4381 cggggtcgcg acggtcgttc ccgtatcgcg catatggttg agcacgcggc agacatattc
     4441 ggacacgacg tcggcgcgca gcgtccagct cgcgttgaga tagccgaaca ccgccgagaa
     4501 attcggaatg ttcgagaaca tgcacgcctt gtaataataa tggtcgtgcc aatcgacggg
     4561 ggtgccgtcg acgcgcaccg gaatcttgcc tgcgaccgcc agcttcaggc ccgtcgcggt
     4621 gatgatgatg tcggcgtcga gatgcttgcc cgatttaagc tggatgcccg ccgcgtcgaa
     4681 cttctcgata tgatcggtca cgaccgatgc ctcgcccgcc ttcatcgctt cgaagaaatc
     4741 ggcgtcgggg acgaggcaga gccgctgttc ccacgggttg tacggcgggg tgaaagcctc
     4801 ggcgtcataa tgatcgccga ggctcgcctt cagcttcttg gtcaggaact ccttgacctt
     4861 ctcgggc
//
LOCUS       DATMSU010000438         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_7958, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000438 DATMSU010000000
VERSION     DATMSU010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7958"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..472
                     /locus_tag="VN034_11730"
     CDS             149..472
                     /locus_tag="VN034_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF736 family protein"
                     /protein_id="HWV61320.1"
                     /translation="MAAIGFVNGTIEKGFVGQLKTLSIRASIEISPNRTKGNNVQPDF
                     RVFSEGVEIGAGWVKVGELSGKTYVSLSLAAPEFGPRRLYANLGRAAGQDDDDTYAVI
                     WNPAD"
     gene            558..1460
                     /locus_tag="VN034_11735"
     CDS             558..1460
                     /locus_tag="VN034_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HWV61321.1"
                     /translation="MKTDLDHLPDRKKRDLARIVEILFAEFEDATALSTQKWKKQGRI
                     LKIILYGSHARGDWVADPKGGYYSDYDILVVVSDERLTDPVDYWYKAEDVFLREYSIS
                     KRLSAPVGLIVHSLGDVNFQLSRGRPFFIDIVRDGVVLYEFGTQSFDPPKPLSAEVAR
                     EEAQGYFDQWFASSSEFLDGARYFISRGYSNRAAFLLHQATEHFYHTVLNTLTLYSPK
                     SHKINFLRDKAEDIARELIPVWPRDEKFARRCFELLQQAYVNARYSPHYKITDGELEW
                     LVGRIEMLQSAVKSIAERHLADAG"
     gene            complement(1457..1657)
                     /locus_tag="VN034_11740"
     CDS             complement(1457..1657)
                     /locus_tag="VN034_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61322.1"
                     /translation="MQHDVRLRAAARAIYDACYPGEEWAPVGFEEAERCRTIHYRQAV
                     GAAIEARAAFVAPVQPGLFDPG"
     gene            1859..2095
                     /locus_tag="VN034_11745"
     CDS             1859..2095
                     /locus_tag="VN034_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61323.1"
                     /translation="MALPNDDAAARAARARLGTPFLSPEQAAFYLGLTTRTLQGYRSR
                     RTGPRFRRHGRFIRYHIDDIDAWALRSSKDDGDA"
     gene            2088..>2299
                     /locus_tag="VN034_11750"
     CDS             2088..>2299
                     /locus_tag="VN034_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61324.1"
                     /translation="MRDALSGSAGGALFSIRRRMLWPGIAACLVVAMGTTSILTPRPR
                     LIWNASASAPVGLWRVAPDAPFGRGD"
BASE COUNT          393 a          787 c          703 g          416 t
ORIGIN      
        1 ccgccgcgcc tgcgcggcca aaaaagcgcg ggcgcgtccg tcctccgctg cgctgcggcc
       61 ctgcgggtgc gcgccggttc cttgccgccc gtcctctttc cgcccacgct ccggagatgg
      121 tctccggcag cgatgaagga gacgaacgat ggcagccatc ggctttgtga acggcacgat
      181 cgaaaagggt ttcgtcggcc agttgaagac gctctcgatc cgcgcgagca tcgagatcag
      241 ccccaaccgc accaagggga acaatgtcca gcccgacttc cgggtctttt ccgaaggcgt
      301 cgagatcggt gcgggatggg tcaaggtcgg cgaactgtcg ggcaagacct atgtctcgct
      361 gagcctcgcc gcgcccgaat tcggaccgcg ccggctctac gcgaacctcg gccgcgccgc
      421 ggggcaggac gatgacgaca cctatgccgt catctggaat cccgccgact gaagccggtc
      481 cccgcgcccg ttccggcgga cgcggggatc acccttttcc ttgtcgcggc gcgatattcg
      541 gctaggattc cagccccatg aaaaccgatc tcgaccatct tcccgaccgc aagaagcgtg
      601 atctcgcgcg catcgtcgaa atcctcttcg ccgaattcga ggatgcgacc gcgctttcga
      661 cgcagaaatg gaagaagcag gggcgcatcc tcaagatcat cctctacggc agccatgcgc
      721 gtggcgactg ggtcgccgat ccgaagggcg gctattattc ggactatgac atcctcgtcg
      781 tggtgagcga cgagcggctc accgatccgg tcgactattg gtacaaggcc gaggacgtct
      841 tcctgcgcga atacagcatc tcgaaacggc tgtcggcgcc cgtcgggctg atcgtccaca
      901 gcctcggcga cgtcaatttc cagctgtcgc gcgggcggcc tttcttcatc gatatcgtgc
      961 gcgacggagt cgtgctctac gagttcggaa cccagtcgtt cgatccgccg aagccgctgt
     1021 cggccgaggt cgcgcgcgag gaagcgcagg gatattttga ccagtggttt gccagctcta
     1081 gcgagtttct cgacggcgcc agatacttta tttcccgagg ctacagcaac cgcgcggcct
     1141 ttctccttca ccaagcgacc gagcatttct atcacaccgt gctcaacacc ctgaccctct
     1201 attcgcccaa atcgcacaag atcaatttcc tccgcgacaa ggccgaggat atcgcgcgcg
     1261 agctgattcc ggtgtggccg cgcgacgaga aattcgcgcg ccgctgcttc gaactgctcc
     1321 agcaggccta tgtcaacgcg cgctattcgc cgcactacaa gatcaccgac ggcgagctcg
     1381 aatggctcgt cggacgcatc gagatgctcc agtcggccgt gaaatcgatc gccgaacgtc
     1441 acctcgcgga cgccggttag cctggatcga acagtccggg ctgcaccggc gcgacgaacg
     1501 ccgcgcgtgc ctcgatcgcc gcgccgaccg cctgccggta atggatcgtc cggcagcgtt
     1561 cggcttcctc gaagccgacc ggggcccatt cctcgcccgg atagcaggcg tcatagatcg
     1621 cgcgcgcagc ggcgcgcagc cgcacgtcgt gctgcacttc tcgtctccat atcgccctcg
     1681 aatcgcccgt cgtgcgggga cgccgacgat tagaacatag agagaacata tccgcaagtc
     1741 gcagggggat cgaatttcgc accggcgatt ttcgagcctc cccggcgagg ggattccgcc
     1801 gcaccgacat cactcccgag gcgccgcgag gcgtccttct tcttcgagga gcgtgatgat
     1861 ggccttaccg aatgacgatg ccgccgcgcg cgccgccagg gcgcggctcg gcacgccctt
     1921 tctcagtccc gagcaggcgg ccttctatct cgggctgacg acgcgcacct tgcagggata
     1981 tcgatcgcgc cgaaccggcc cgcgctttcg ccgccacggc cgctttatcc gctaccatat
     2041 cgacgacatc gacgcctggg cgttgcgttc atcgaaggac gatggcgatg cgtgacgcgc
     2101 tgtccgggag cgccggcggt gcgctttttt ccattcgccg gcggatgctt tggcccggca
     2161 tcgcggcttg cctcgtcgtc gcgatgggca cgacgagtat cctgactccg cgcccgcgcc
     2221 tgatctggaa cgccagcgcg agcgcaccgg tcggcctgtg gcgcgtcgcg cccgatgcgc
     2281 cgttcggccg cggcgacat
//
LOCUS       DATMSU010000439         3805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_102135,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000439 DATMSU010000000
VERSION     DATMSU010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102135"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VN034_11755"
     CDS             <1..300
                     /locus_tag="VN034_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HWV61325.1"
                     /translation="GRSGRNAEYLLALALELGGTPGISALAADTDGLDGTEDNAGAII
                     GPETLAAAAQRGIDAAAALTDNDGYGFFAELGALLVTGPTRTNVNDFRAILIDPI"
     gene            312..1388
                     /locus_tag="VN034_11760"
     CDS             312..1388
                     /locus_tag="VN034_11760"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HWV61326.1"
                     /translation="MTSDTTHRIAVIPGDGIGKEVVPEGLRVLEAVERKFGCRFAFDQ
                     FDFASCDYYLAHGQMMPGDWKAQIGGHDAIFFGAVGWPDSVPDHVSLWGSLIQFRREF
                     DQYVNLRPVKLMPGVPSPLAGREPGDIDFYVVRENTEGEYSSIGGKIFPGTDREVVMQ
                     ETVMTRIGTDRILKFAFELAQRREKRHLTSATKSNGISITMPYWDERVEAMAAHYPEV
                     RWDKYHIDILTAHFVQHPDWFDVVVASNLFGDILSDLGPACTGTIGIAPAGNINPERH
                     FPSLFEPVHGSAPDIAGQGIANPVGQIWSASLMLDHLGEHEAAKAVLGAIEETLACPQ
                     YRTRDLGGAASTGECTNAIIDALE"
     gene            complement(1449..2090)
                     /locus_tag="VN034_11765"
     CDS             complement(1449..2090)
                     /locus_tag="VN034_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HWV61327.1"
                     /translation="MTSEAIRDPKTDHLLTPQNSAFIIIDYQPVQVNSIASMDRQLLV
                     NNIAGAAKAAVAYDLPIIHSTVNVETGLNKPPVPQIRKVLGDYPTYDRTTINSWEDVE
                     FRKAVEATGRRKLIMTALWTEACLTFPALDALAEGYEVYVPVDAVGGTSLAAHDAALR
                     RIEQAGGKMISVPQLFCELQRDWKRSETVPAFMNLFIETGGTAGIQFSYDKAD"
     gene            complement(2223..3089)
                     /locus_tag="VN034_11770"
     CDS             complement(2223..3089)
                     /locus_tag="VN034_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWV61328.1"
                     /translation="MKTILGTYSNPNQHWVGDGFPVRSLFSYNSLGGQVSPFLLLDYA
                     GPYNFAPTTSRRGVGQHPHRGFETVTIVYDGEVEHKDSAGNGGIIGPGDVQWMTAAGG
                     ILHEEYHSSAFGKTGGPFRMVQLWVNLPAKDKMAPGGYQGIAAADIPTVELPGGAGFG
                     RVIAGELLGAKGPARTFTPINVWDVRLNRDADLTLDLPEGHTAMLVVLGGHVTVNGSE
                     GVGEAEMLLLSREGDAVRIHADGDAVILVLTGEPIDEPIVGYGPFVMNSEAEIRQAID
                     DYNNGRLATAAA"
     gene            3202..>3805
                     /locus_tag="VN034_11775"
     CDS             3202..>3805
                     /locus_tag="VN034_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV61329.1"
                     /translation="MQDLNDLYLFVQAVDHGGFAAAARALGMQKSKLSRRIGLLEERL
                     GVRLIQRSTRRFSVTEIGREYYRHCVAMLVEAEAAQTVVDQSRAAPRGVIRLSCPTGL
                     IAFQFGDLFGRFMALHPDVELHVASTNRRVDVIGEGFDLAIRVRPPPIAESELVMRRF
                     DERTIRIVASPALLRGRSIAAPADLAGLPSLDFGPAGGEHR"
BASE COUNT          657 a         1236 c         1218 g          694 t
ORIGIN      
        1 ggacgcagcg ggcgcaacgc cgaatatctg ctcgccttgg cgctcgaact cggcggcacg
       61 ccaggcatca gcgcgctcgc cgccgacacc gacgggctcg acgggaccga ggacaatgcc
      121 ggagcgatca tcgggcccga aacgctcgcc gcggccgcgc aacgcggtat cgatgcggcg
      181 gcggcgctta ccgacaatga cggctacggc tttttcgcgg agcttggcgc gctgctcgtc
      241 accgggccga cgcgaaccaa tgtgaatgat ttccgcgcca tcctgatcga tcccatttga
      301 gcgaggttaa aatgacaagc gacacgacac accgcatcgc cgtgatcccg ggcgacggga
      361 tcggcaagga agtggtgccg gaagggcttc gcgttttgga agcggtcgag cggaaattcg
      421 gctgccgctt cgccttcgac cagttcgatt tcgcctcgtg cgattattat ctggcgcacg
      481 gccagatgat gccgggcgac tggaaggccc aaatcggcgg ccatgacgcg atcttcttcg
      541 gcgccgtcgg ctggcccgac agcgtccccg atcatgtctc gctatggggt tcgctgatcc
      601 agttccgccg cgaattcgac caatatgtca atcttcggcc ggtcaagctc atgccaggcg
      661 tccccagccc gctcgcgggc cgcgagccgg gcgacatcga cttctatgtc gtccgcgaaa
      721 ataccgaagg cgaatattcg tcgatcggcg gcaagatttt tccgggcacc gaccgcgaag
      781 tcgtcatgca ggaaacggtg atgacgcgga tcggcaccga ccgcatcctg aaattcgcct
      841 tcgagctcgc gcagcggcgc gaaaaacgcc atctcacctc ggcgaccaag tcaaacggca
      901 tttcgatcac catgccctat tgggacgaac gggtcgaagc gatggcggcg cattatcccg
      961 aggtccgctg ggacaaatat catatcgaca ttctgaccgc gcatttcgtc cagcaccccg
     1021 attggttcga cgtcgtggtc gcgtccaacc tgttcggcga catcctgtca gacctcggcc
     1081 cggcctgcac cgggacgatc ggtatcgcac ccgccggaaa catcaatccc gagcggcact
     1141 tcccttcgct gttcgaaccc gtccacggct cggcgcccga tattgcgggc cagggaattg
     1201 ccaatccggt cgggcagatc tggtcggcgt cgctgatgct cgaccacctt ggcgagcacg
     1261 aagcggccaa agcggtccta ggcgcaatcg aagagacact cgcctgcccg caataccgca
     1321 cacgcgacct cggcggcgcg gcaagcacgg gtgagtgcac caacgctata atcgacgcac
     1381 tcgaatgagc gtccccgatc gcgacgcccc tcctggcggt gccggcaggc cggcgaggct
     1441 ctcggggatc agtcggcctt gtcgtatgag aattgtattc cggcggtgcc gcccgtctcg
     1501 atgaagaggt tcatgaaggc gggaacggtc tcgctgcgct tccagtcgcg ctggagttcg
     1561 caaaagagct gcggaaccga gatcatcttg ccgccggcct gctcgatacg gcggagtgcc
     1621 gcgtcgtgcg ccgcgagcga ggttccgccg acggcgtcga ccgggacata gacctcatag
     1681 ccttcggcga gcgcatcgag cgccggaaag gtcaggcagg cctcggtcca cagtgcggtc
     1741 atgatcagct tccggcgccc ggtcgcctcg accgccttgc ggaactcgac gtcttcccag
     1801 ctattgatcg tcgtgcggtc gtaggtcgga tagtcgccca gcaccttgcg gatttgcggc
     1861 accggcggct tgttgagccc cgtttcgaca ttcactgtcg aatggatgat cgggagatcg
     1921 taggcgacgg ccgccttcgc ggcgccggcg atattgttga ccagcaactg ccggtccatc
     1981 gaggcgatcg aattcacctg taccggttga tagtcgatga tgatgaaggc cgaattctgc
     2041 ggagtcagca gatgatcggt cttcgggtca cggatggctt cactcgtcat gggtcttctc
     2101 ctgctttggg gcgtgcgcaa ggggcggcag cctcctgccg tcacggcggg cacacgaccg
     2161 tcgcgatttt ccgaaaggga gagcgttgga cccggctccc acggacggga gccgggcacg
     2221 cgtcaggctg cggctgtcgc aagcctgccg ttgttgtagt cgtcgatcgc ctgacggatt
     2281 tccgcctcgc tgttcatcac gaagggtcca tagccgacga tcggttcatc gattggttcg
     2341 ccggtcagga cgaggatcac cgcatcgccg tcggcatgga tgcgtaccgc gtcgccttcg
     2401 cggctcagca gcagcatctc cgcctcgccc acaccctcgc tcccgtttac ggtgacgtgg
     2461 ccgccgagca cgacgagcat cgcggtgtgg ccttcgggca ggtcgagcgt caggtcggcg
     2521 tcgcggttga gccggacgtc ccagacgttg atcggcgtga aagtccgggc ggggcccttc
     2581 gcgccgagca attcgcccgc gatcacgcgg ccgaagccgg cgccgccggg cagttcgacg
     2641 gtggggatgt ctgccgcggc gatgccctga taaccgcccg gcgccatctt gtccttcgcg
     2701 ggcagattga cccacagctg gaccatcctg aacggcccgc cggtcttgcc gaatgcggac
     2761 gagtgatatt cctcgtgcag gatgccgccc gcggcggtca tccactggac gtcgccgggg
     2821 ccgatgatgc cgccgttgcc cgccgaatcc ttgtgctcga cctcaccgtc atagacgatc
     2881 gtgaccgtct cgaacccgcg gtgcggatgc tggccgaccc cgcgccggct ggtcgtcggc
     2941 gcaaaattat agggcccggc atagtcgagc agcaggaagg gactcacctg cccgccgaga
     3001 ctgttgtacg aaaagagcga gcgcaccgga aagccgtcgc cgacccaatg ctggttcggg
     3061 ttgctgtagg tgccgaggat cgttttcatg cttcatctcc tgcgcctccc gatgggaagg
     3121 cgtctgttgc gaagacaata atgccgcatc aatgaagcca gaagattgtg attccggact
     3181 cagcgtccta aaaatcggac gatgcaggac ctcaacgatc tctatctctt tgtccaggcg
     3241 gtcgatcatg gcggtttcgc tgcggcggcg cgcgcgctgg ggatgcagaa gtcaaagctc
     3301 agccgccgca tcgggttgct cgaggagcgg ctcggcgtac ggctgatcca gcggtcgacg
     3361 cggcgctttt cggtcaccga gatcggccgc gaatattatc gccattgcgt cgcgatgttg
     3421 gtcgaggcgg aggcggcgca gaccgtcgtc gaccagtcgc gcgcggcacc gcgcggcgtc
     3481 atccggctca gctgtccgac gggactgatc gccttccaat tcggcgatct gttcgggcgt
     3541 ttcatggcgc ttcaccccga tgtcgaactg catgtcgcaa gcaccaaccg ccgcgtcgat
     3601 gtgatcggcg aaggcttcga cctcgcgatc cgcgtccgcc cgccgccgat cgccgagagc
     3661 gaactggtga tgcgccgctt cgacgaacgc accattcgca tcgtcgccag ccccgcgttg
     3721 ctacgcgggc ggagcatagc ggcccccgcc gacctcgcgg gcctgcccag tctcgatttc
     3781 ggcccggcgg gcggcgagca tcgct
//
LOCUS       DATMSU010000440         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_168163,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000440 DATMSU010000000
VERSION     DATMSU010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168163"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="ruvB"
                     /locus_tag="VN034_11780"
     CDS             complement(<1..656)
                     /gene="ruvB"
                     /locus_tag="VN034_11780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWV61330.1"
                     /translation="MTDLTTPIRTPEDADAALRPKSLAEFVGQAAARENLRIFIEAAK
                     ARADALDHVLFYGPPGLGKTTLAQIVAKELGVGFRSTSGPVIAKAGDLAALLTNLEDG
                     DVLFIDEIHRLSPAVEEILYPAMEDRALDIMIGEGPSARSVRIDLPKFTLVGATTRQG
                     LLTTPLRDRFGIPVRLNFYTHAELEQVISRAARLLALPIAPDGALEIAKRSRGTPRIA
                     G"
     gene            complement(682..1023)
                     /locus_tag="VN034_11785"
     CDS             complement(682..1023)
                     /locus_tag="VN034_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HWV61331.1"
                     /translation="MRDVFQPCIYILASYLNGTLYIGVTSNLVQRMWQHRNGMMKGFT
                     TRYRTHRLVHFELFDTMHEAISREKQLKRWHRQWKINLIEADNPEWRDLAFDIGAGPI
                     GRAMDAETSSA"
     gene            complement(1092..1502)
                     /locus_tag="VN034_11790"
     CDS             complement(1092..1502)
                     /locus_tag="VN034_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV61332.1"
                     /translation="MTDRIRHTCCRCGQIRIACTGEPIRTSVCHCRECKARSGSAFAA
                     QVRFPAEQVSIEGEAHARMWQYTGDSGNTADFFFCGTCGSGVWYRARPYHDAYAIPLG
                     NFEPGHGFIPDYSVYEERKEPWVSIAGDEIEHYD"
     gene            complement(1499..>1615)
                     /locus_tag="VN034_11795"
     CDS             complement(1499..>1615)
                     /locus_tag="VN034_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWV61333.1"
                     /translation="LGFKPGEASAAVAAASEELGAGASLDALVRAALKKAAK"
BASE COUNT          281 a          494 c          535 g          305 t
ORIGIN      
        1 cccgcgatgc gcggcgtccc gcgtgaccgc ttcgcgattt cgagtgcgcc gtcgggggcg
       61 atcggcagcg ccagcaggcg cgcggcgcgc gaaatcacct gttcgagttc ggcgtgggta
      121 tagaaattga gccgcaccgg gataccgaac cggtcgcgca gcggcgtcgt cagcagtcct
      181 tgtctggtcg tcgcgccgac cagcgtgaat ttcggcaggt cgatacggac gctgcgcgcc
      241 gacggacctt cgccgatcat gatatcgagc gcgcgatcct ccatcgcggg atagagaatt
      301 tcctcgaccg cgggcgacag ccggtgaatc tcgtcgatga acaggacgtc gccgtcctcg
      361 agattggtga gcaatgccgc gaggtcgccc gccttcgcga tcaccgggcc gctggtcgac
      421 cggaagccga cgccgagttc cttggcgacg atctgcgcca gcgtcgtctt gccgagaccc
      481 ggcgggccat agaagagcac atggtcgagc gcgtccgcac gcgccttcgc cgcctcgatg
      541 aagatgcgca gattctcgcg cgccgccgcc tgcccgacga attcggcgag cgacttgggt
      601 cgcagcgcgg cgtcggcatc ctcgggggtg cggatggggg tggtgaggtc ggtcacggtt
      661 ttgcgatacc cataatcttc gtcatgctga acttgtttca gcatccatgg cccgaccgat
      721 cggtccagcg ccgatgtcga aggctagatc gcgccattcg ggattgtcgg cctcaatgag
      781 gttgatcttc cattggcgat gccagcgttt caattgtttt tcgcgggaaa tggcttcgtg
      841 catcgtgtcg aacagctcga aatggactag ccggtgggtc cggtatcttg tcgtaaagcc
      901 cttcatcatt ccgttgcggt gctgccacat gcgctgaacg agatttgatg tgacaccgat
      961 atacagcgtg ccattgaggt agctggcgag aatgtagatg catggctgaa aaacgtcgcg
     1021 catgaccgat cgctcagggc catggatgct gaaacaagtt cagcatgacg gagaagagcg
     1081 gcggcgtgca gtcagtcata atgctcgatc tcgtcgccag cgatcgaaac ccaaggctcc
     1141 ttgcgctctt catagaccga atagtcgggg atgaagccgt ggccgggttc gaaattgccg
     1201 agcgggatcg cataggcgtc atgataaggc cgcgcgcggt accagacgcc cgaaccacag
     1261 gtgccgcaga agaagaagtc ggcggtgttg ccgctgtcgc cggtgtactg ccacatccgg
     1321 gcatgcgctt cgccctcgat gctgacctgc tcggccggga agcgaacctg cgccgcgaag
     1381 gcgctgccgc tccgcgcctt gcattcgcgg cagtggcaga ccgaggtgcg gatcggctcg
     1441 cccgtgcagg caatgcggat ctggccacag cggcagcacg tgtggcggat gcggtcggtc
     1501 acttcgccgc cttcttcagc gccgcacgga cgagcgcgtc gaggcttgcg cccgcgccca
     1561 gttcctcgct cgctgcagcg accgccgcgc tcgcttcgcc gggtttgaag ccgag
//
LOCUS       DATMSU010000441         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_168164,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000441 DATMSU010000000
VERSION     DATMSU010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168164"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1191)
                     /locus_tag="VN034_11800"
     CDS             complement(169..1191)
                     /locus_tag="VN034_11800"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HWV61334.1"
                     /translation="MPDLTLLFRLARSARSLPLLAVFTLAGFAAPAAAEDAALPYDLP
                     AIGDTTLATGDEEQAAAHFQNTEIDENILLPPARPQDDDPERKWSRDYFAVAVGVLNT
                     PKYNGSDDRRTLPAFYFRGRVSGFSFSTRGTNLVVDLIRQRRGQRTDLKFGPTISLRG
                     DRSGKIDDPQVAALGKRKVAVELGLFAGVTQTGVLTGKYDQLGFRMSASKDVSGRHGS
                     WTASPTIDYGTPLSKRAYIGVSASVNFYGKGFGRYYYDIDAEDSAASGLPVYDGAGRK
                     ATAGKYTLGIAGAYSLSGDLRKGFTLIGGAQYGRIASRFAKSPIVADVGSADQWLFGG
                     GLAYQF"
     gene            1388..1630
                     /locus_tag="VN034_11805"
     CDS             1388..1630
                     /locus_tag="VN034_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61335.1"
                     /translation="MSRIPRLVGYGFMAAAALVAAVMKKEGVATIGPLPAVAVALFLG
                     MVGVMLVFTDLMVRGLYAQVDAAKRREEEGEPSERD"
     gene            1677..3605
                     /gene="dxs"
                     /locus_tag="VN034_11810"
     CDS             1677..3605
                     /gene="dxs"
                     /locus_tag="VN034_11810"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWV61336.1"
                     /translation="MTDRPHTPLLDTVDVPADLRKLKPEDLRQFADELRAEMISAVGT
                     TGGHLGSGLGVVELTTALHYVFDTPRDKIVWDVGHQCYPHKIITGRRDRIRTLRTGGG
                     LSGFTKRSESEYDPFGAAHSSTSISAALGFAVANKLTGAPGRAIAVIGDGSMSAGMAY
                     EAMNNAKQAGNRLIVILNDNDMSIAPPVGGLSAYLAKLVSSRPFVELREIARRFARKL
                     PEPLHKAAKKTDEFARGMAMGGTLFEELGFYYVGPIDGHNLEHLIPVLENVRDSEHGP
                     VLIHAVTKKGKGYAPAEAAADKYHGVQKFDVITGEQAKAPPGPPAYQNVFGETLAKLA
                     ESDKRIVAITAAMPSGTGVDKFAKAHPERSFDVGIAEQHAVTFAAGLAAQGMRPFAAI
                     YSTFLQRAYDQVVHDVAIQNLPVRFAIDRAGLVGADGSTHAGSFDVTYLATLPNFVVM
                     AAADEAELVHMTYTAAEHDAGPIAFRYPRGNGTGVPLPDVPEKLEIGKGRVVRSGKTV
                     AIFSLGTRLAEALKAADTLEARGLSTSVIDLRFAKPLDEELIRKTLAAHEVCVTIEEG
                     SIGGLGAHVLTLASDEGLIDAGLKLRTMRLPDVFQDQDKPELQYDEAGLNAPQIVDTV
                     LKALRHNSAGVEEAGARA"
     gene            3602..4081
                     /locus_tag="VN034_11815"
     CDS             3602..4081
                     /locus_tag="VN034_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61337.1"
                     /translation="MTIWVTLDWDAIATASAGILAVGSALFVARKQQRIKEQELRLSL
                     LQERRQLVGRFRKFQASMMRLRNLDDEAVSDFWLLVQEVQLYFDPATARSVEDVFDEV
                     WNYIGTAVSIRIYRDDEMHEEARAEVDKRFELLKSLNRKFPVAIKLMVEKTRVPEHI"
     gene            4084..4752
                     /locus_tag="VN034_11820"
     CDS             4084..4752
                     /locus_tag="VN034_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61338.1"
                     /translation="MNFFNLLQSLDELLYEVMSWLVFYPITLWRTLVRPLKMMDYSDL
                     EQGDRADQQYTDTLSPPLFLLLSLILVHAAEIALIGNDAVVASKVGLSALVSTNTDLI
                     ILRIVSYSLFPVMMATRMVRAQRLQVNRDTLRAPFYSQCYVVAVLAMMLGSAVILIKL
                     AHDWSVLAGGALVLFALLWFGFLQTAWFNQHLKCGRLKAFGHASRAMVESLAAMLLLS
                     SLFS"
     gene            complement(4761..5195)
                     /locus_tag="VN034_11825"
     CDS             complement(4761..5195)
                     /locus_tag="VN034_11825"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWV61339.1"
                     /translation="MRGHRFANNDEAVAYMKSVAVDASGFTAFLGVEPLRCFDGESEL
                     LLALRPDMTQHHGYAHGAIVGLMADNACAWAASSVVGDVVTGSYTINFLAPAKGSRLR
                     AKGSVVKAGKRQAIVRADVWAESDGEEPRRCAIAQATIVPVA"
     gene            5285..>5604
                     /locus_tag="VN034_11830"
     CDS             5285..>5604
                     /locus_tag="VN034_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61340.1"
                     /translation="MPSRSPCAVGRASRLLGDRWVLLILREAFLGIDRFEDFLGRLDI
                     SRAALTSRLGWMVEAGVLERVPPEGKRALYRLTPAGEALRPTYDAIKAWGDIWLPRRA
                     KENGP"
BASE COUNT          989 a         1752 c         1848 g         1015 t
ORIGIN      
        1 gttgaggtcg tgagctgatt tcccctcccg ccggcgggag gggcagcgag acttacgaac
       61 tttgttcgtt agtcgcagcg gggtgggcat tcgcaccgtt gctggcccac ccccagcccc
      121 tcccgcaagc gggaggggag aagaagatcc gcttccggcc caaacccctc agaattgata
      181 ggcgagcccg ccgccgaaaa gccattggtc ggcgctgccg acgtccgcca cgatcggcga
      241 tttggcgaag cgcgaggcga tgcggccata ttgcgcgccg ccgatcagcg tgaacccctt
      301 tctgaggtcg cccgacagcg aataggcgcc cgcgatgccg agcgtatatt tgcccgcggt
      361 cgccttgcgc cccgcgccgt catagacggg gagcccgctc gcggcgctgt cttcggcatc
      421 gatgtcgtaa taatagcggc cgaaaccctt gccgtagaaa ttcaccgacg ccgaaacgcc
      481 gatataggcg cgtttcgaca gcggcgtgcc ataatcgatc gtcggcgacg ccgtccagct
      541 gccgtggcgc cccgaaacat ccttcgacgc cgacatgcga aagccgagct ggtcatattt
      601 gccggtgagc acgccggtct gggtgacccc cgcgaacagc ccgagctcga ccgcgacctt
      661 gcgcttgccg agcgccgcga cctgcggatc gtcgatcttg ccgctgcggt cgccgcgcag
      721 gctgatcgtc gggccgaatt tgaggtcggt ccgctgcccg cgccgctgac ggatcaggtc
      781 gaccacgaga ttggtgccgc gcgtcgaaaa ggaaaagccg ctgacccgcc cgcgaaaata
      841 aaaggcgggc agcgtgcggc gatcgtcgga gccgttatat ttcggcgtgt tcaatacgcc
      901 gacggcgacc gcgaaatagt cgcgcgacca tttgcgttcg ggatcgtcgt cctgcggccg
      961 cgcggggggc agcaggatat tctcgtcgat ctcggtgttc tggaaatggg cggccgcttg
     1021 ttcctcgtca ccagtggcga gcgtcgtgtc gccgatcgcc ggcaggtcat agggaagcgc
     1081 ggcatcctcg gcggcggcgg gcgcggcgaa accggcgagg gtaaagacag caagaagagg
     1141 gagcgatcgg gccgaacggg ccaagcggaa caacaaggtc aaatcgggca taaacatccg
     1201 cctatatctc tgtgtctata cccctcttta cgggggtgta agtaaacgac gcgcctttgc
     1261 aacggcttcg tcttgcggag gcacgcattt cgccgcatgt ggctttcatg tcacgatgct
     1321 ccttcgccgc tctcatattc ctccgcagcg ccattggttc ccttgggctg gcgcggattc
     1381 gcccggcatg agccggattc cgcgcctggt cggttatggc ttcatggcgg cggcggcgct
     1441 ggtggccgcg gtgatgaaga aggagggggt cgcgacgatc gggccgctgc ccgccgtcgc
     1501 ggtcgcgctg ttcctcggca tggtcggggt gatgctcgtg ttcaccgacc tgatggtgcg
     1561 cgggctctat gcgcaggtcg atgccgccaa acggcgcgag gaagagggcg agccgagcga
     1621 gcgcgactga tcccttttcg ttcgacacta tcggcggtta ccagtatgac ggcgcgatga
     1681 ccgatcgtcc gcatacgccg ctgctcgaca cggtggatgt ccccgccgac ctccgcaagc
     1741 tgaagcccga agacctccgc cagttcgccg acgaattgcg cgccgagatg atctcggcgg
     1801 tcggcacgac gggcgggcat ctgggctcgg ggctcggcgt cgtcgagctg accaccgcgc
     1861 tccactatgt gttcgacacg ccgcgcgaca agatcgtgtg ggacgtcggc caccaatgct
     1921 atccgcacaa gatcatcacc gggcgccgcg accgcatccg gacattgcgg accggcggcg
     1981 ggctgtcggg tttcaccaag cgcagcgaga gcgaatatga tccgttcggc gcggcgcaca
     2041 gctcgacctc gatcagcgcc gcgctcggct tcgcggtcgc caacaagctg accggcgccc
     2101 ccgggcgcgc gatcgcggtg atcggcgacg ggtcgatgtc ggcgggcatg gcctatgagg
     2161 cgatgaacaa cgccaagcag gcgggcaacc ggctgatcgt catcctcaac gacaacgaca
     2221 tgtcgatcgc gccgcccgtt ggagggctct cggcctatct ggcgaagctc gtgtcgtcgc
     2281 ggccgttcgt cgagctgcgc gagatcgcac ggcgctttgc gcgcaagctg cccgagccgc
     2341 tgcacaaggc ggcgaaaaag accgacgagt tcgcacgcgg catggcgatg ggcgggacgc
     2401 tcttcgagga actcggcttc tattatgtcg ggccgatcga cgggcataat ctcgagcatc
     2461 tgatcccggt gctcgaaaat gtccgcgaca gcgagcatgg gccggtgctg atccatgcgg
     2521 tgaccaagaa gggcaagggc tatgcccccg ccgaggcggc ggccgacaaa tatcacggcg
     2581 tgcagaaatt cgacgtcatc accggcgagc aggcgaaggc gccgcccggg ccgcccgcct
     2641 atcagaatgt cttcggcgaa acgctcgcca agctcgccga gagcgacaag cgcatcgtcg
     2701 cgatcactgc ggcgatgccg tcgggcaccg gggtcgacaa attcgcgaag gcgcatcccg
     2761 aacgcagctt cgacgtcggc atcgccgagc agcatgcggt gaccttcgcg gcggggctcg
     2821 cggcgcaggg catgcggccg ttcgcggcga tttattcgac cttcctccag cgcgcctatg
     2881 atcaggtcgt ccacgatgtc gcgatccaga atctgccggt gcgtttcgcg atcgaccgcg
     2941 cggggctcgt cggtgccgac ggttcgacgc acgcgggctc gttcgacgtc acctatctcg
     3001 cgaccttgcc caattttgtc gtgatggcgg cggccgacga ggccgaactg gtccatatga
     3061 cctataccgc cgccgaacat gacgcgggcc cgatcgcctt ccgctatccg cgcggcaacg
     3121 gcaccggggt gccgctgccc gacgttcccg aaaagctgga gatcggcaag gggcgcgtcg
     3181 tgcgcagcgg caagaccgtc gcgatcttct cgctcggcac gcggctggcc gaggcgctca
     3241 aggcggccga cacgctggag gcgcgcgggc tgtcgacgag cgtaatcgac ctgcgcttcg
     3301 cgaagccgct cgacgaggag ctgattcgca agacgctcgc cgcccacgag gtatgcgtga
     3361 cgattgagga aggcagcatc ggcggattgg gcgcgcatgt gctgacgctc gcgagcgacg
     3421 aggggctgat cgacgcgggg cttaaactgc gcacgatgcg cctgcccgac gtcttccagg
     3481 atcaggacaa gcccgagctg caatatgacg aagcgggact caatgcgccg cagatcgtcg
     3541 acacggtgct caaggcgctg cgccacaaca gcgcgggggt cgaagaggcc ggcgcgcggg
     3601 cttgacgata tgggtcaccc ttgattggga cgctatcgcg actgcctctg cagggatttt
     3661 ggccgtagga tcggcacttt tcgttgctcg gaagcagcag cgaataaagg agcaagagct
     3721 ccgcttgtcg cttctccaag agaggcggca actggttggg cggtttagaa aattccaagc
     3781 ctcaatgatg agattgcgca atcttgatga tgaggcggtc tccgattttt ggctcctcgt
     3841 tcaagaagtg cagctctatt tcgaccccgc caccgcgcgg tcggtcgaag atgtcttcga
     3901 tgaggtctgg aactatatcg gtactgcagt ttcgatcaga atatatcgag atgatgaaat
     3961 gcacgaagag gcccgtgctg aggtcgataa acgtttcgaa ctcttgaaga gcctaaaccg
     4021 aaaatttccg gtagcgatca agctgatggt cgaaaaaacg cgcgtgccgg agcacatctg
     4081 accatgaact ttttcaacct cctgcaatcg ctcgacgagc tgctttacga ggtgatgtcg
     4141 tggctggtct tctatccgat caccttgtgg cgcaccttgg tgcggccgct gaagatgatg
     4201 gactattcgg acctcgagca gggcgaccgg gcggaccagc aatataccga cacgctgagc
     4261 ccgccgctgt tcctgctgct atcgctgatc ctggtccacg ccgccgagat cgcgctgatc
     4321 ggcaatgatg cggtggtcgc gagcaaggtc gggctgtcgg cgctggtcag caccaacacc
     4381 gacctgatca tcctgcgtat cgtcagttac agcctgttcc cggtgatgat ggcgacgcgg
     4441 atggttcgtg cgcagagatt gcaggtcaat cgcgacacgc tgcgcgcgcc cttttacagc
     4501 caatgctatg tcgtcgcggt gctggcgatg atgctgggga gcgcggtgat cctgatcaag
     4561 ctcgcgcacg actggtcggt gctcgcgggc ggggcgctcg tgcttttcgc gctgctgtgg
     4621 ttcggcttcc tgcagaccgc ctggttcaac cagcatctga aatgcggacg tctcaaagcc
     4681 tttggccacg catcgcgtgc gatggtcgaa agcctcgccg cgatgctcct gctgtcgagc
     4741 ctgttcagct aggccacgcc ctaggcgacc ggaacgatcg tcgcctgcgc gatcgcgcag
     4801 cggcgcggtt cttcgccgtc actttcggcc cagacatcgg cgcggacgat cgcctggcgc
     4861 ttgcccgcct tgaccacgct gcccttggcg cgcagccggc tgcccttggc gggggcgagg
     4921 aagttgatcg tatagctgcc ggtgacgacg tcgccgacga cgctcgacgc cgcccaggcg
     4981 cacgcattgt cggccatcag cccgacgatc gcgccatggg catagccgtg atgctgggtc
     5041 atgtcggggc gcagcgcgag cagcagttcg gactcgccat cgaagcagcg caagggttcg
     5101 acgccgagga aggcggtgaa acccgacgca tcgaccgcga cgcttttcat ataggcaacg
     5161 gcttcgtcat tgttggcaaa gcggtgtccg cgcatgatcc atccctttaa gtctgcaata
     5221 cagacttatt gctgcttgca tcgcgatggc gtcaagtctt aaatccagac ttatgaagga
     5281 ttatgtgccc tcgcgttcgc cctgcgccgt cggccgcgcg tcgcggctgc tcggcgatcg
     5341 ctgggtgctg ctgatcctgc gcgaagcgtt tctggggatc gatcgtttcg aggatttcct
     5401 cggccggctc gacatttcgc gcgccgcgct gacctcgcgg ctcggctgga tggtcgaagc
     5461 cggggtgctc gaacgcgtgc cacccgaggg caagcgcgcg ctgtaccgcc ttacgcccgc
     5521 gggcgaggcg ctgcgcccga cctatgacgc gatcaaggcg tggggcgaca tatggctgcc
     5581 gcgccgggcc aaagaaaacg gccc
//
LOCUS       DATMSU010000442         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_178065,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000442 DATMSU010000000
VERSION     DATMSU010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178065"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="VN034_11835"
     CDS             <1..157
                     /locus_tag="VN034_11835"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HWV61341.1"
                     /translation="IDASRIAMIGDGTHVVSLDTVIATMLQTGRDMRDKYKETSKGGL
                     AVSVVEC"
     gene            351..680
                     /locus_tag="VN034_11840"
     CDS             351..680
                     /locus_tag="VN034_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HWV61342.1"
                     /translation="MNYFLLGIAIIAEVVGTSFMKQSEGFTKLAPSLVTVTAYVVAFY
                     CLSLTLKTIPTGVAYAIWSGVGIVLIAAVAWIFQGQRLDAPAMLGMGLIISGVVVMNL
                     FSKVAAH"
     gene            780..1892
                     /locus_tag="VN034_11845"
     CDS             780..1892
                     /locus_tag="VN034_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HWV61343.1"
                     /translation="MKTRAAVAFEAKKPLEIVELDLEGPKAGEVLVEIMATGICHTDA
                     YTLDGFDSEGIFPSVLGHEGAGIVREVGAGVTSVKPGDHVIPLYTPECRQCKSCLSGK
                     TNLCTAIRATQGKGLMPDGTTRFSYKGQPIFHYMGCSTFSNFTVLPEIAVAKIREDAP
                     FQSSCYIGCGVTTGVGAVINTAKVQVGDNVVVFGLGGIGLNVIQGARLAGADKIIGVD
                     INPDREEWGRKFGMTDFLNSKGMSREDTVAAIVAMTDGGADYTFDATGNTEVMRIALE
                     ACHRGWGTSIIIGVAEAGKEIATRPFQLVTGRNWRGTAFGGAKGRTDVPKIVDMYMTG
                     KIEIDPMITHVMGLEEINKGFDLMHAGESIRSVVVY"
     gene            1906..2292
                     /locus_tag="VN034_11850"
     CDS             1906..2292
                     /locus_tag="VN034_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61344.1"
                     /translation="MFSHVALGSDDIAASKRFYDAVLGAFGAPEGVIDERGRLVYMHA
                     GGRLIVTHPVNGEIAACGNGHTLGFLAPSTDAVDAWHAAGVANGGTTAENPPGIRHAT
                     EGRTVYLAYLRDPAGNKLCATHKISG"
     gene            2294..3166
                     /gene="purU"
                     /locus_tag="VN034_11855"
     CDS             2294..3166
                     /gene="purU"
                     /locus_tag="VN034_11855"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWV61345.1"
                     /translation="MGDDRAPAYVLTFSCVDAVGIVAAVTGLLAERDGFILDSQQYAD
                     LDSGRFFMRVEFRGEGPRFPEGLAGVQDAFAPVIARFAMDARISDTAAKPRFVIAVSQ
                     GSHCLNDLLHRWSTGNLAIDVVGVVSNHEAQRRLSEWHGVPFHYLPVSDANRADQEAA
                     ILDVMARGGAEYLVLARYMQVLSSDLSARLAGRCINIHHSFLPGFKGAKPYHRAQERG
                     VKLIGATAHFVTSDLDEGPIIEQAVERVDHRDGVDELIRIGRDTEAQVLARAVRWVAE
                     QRVLIDGRKTVVFR"
     gene            3280..3660
                     /locus_tag="VN034_11860"
     CDS             3280..3660
                     /locus_tag="VN034_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61346.1"
                     /translation="MYSHNMVGSNDIEASKKFYDATFQAIGGKPAIVDDKGRLIYIHN
                     GGLFLVGAPIDGEPATIGNGCTIGFAMDGPEQADAWHKAGVENGGTSIEDPPGVREGG
                     FGAMYLAYLRDPSGNKLCALHRVV"
     gene            3680..4183
                     /locus_tag="VN034_11865"
     CDS             3680..4183
                     /locus_tag="VN034_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HWV61347.1"
                     /translation="MRGEAGLLADLGALAIPFAAHEHEAVFTVAESDAVNAAIPGAHT
                     KNLFLKDNGGAYWLVTVPGAARVDLKALPAAIGSKRVSFGKAEDMERLLGISPGSVTP
                     LAAINAPPGSITVVLDEGLAAAETVNVHPLRNTATLGLPGATILDLLRHWGHRPLVAS
                     IPVQDPA"
     gene            4180..5028
                     /gene="fghA"
                     /locus_tag="VN034_11870"
     CDS             4180..5028
                     /gene="fghA"
                     /locus_tag="VN034_11870"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HWV61348.1"
                     /translation="MTIETLSTVRSHGGTQGVYKHASTTTGTDMTFAVFVPDHEGGAK
                     LPVLWYLSGLTCTHANVMEKGEYRAACAEHGVIFIAPDTSPRGENVPDDPDAAYDFGL
                     GAGFYVDATAEPWAKHYRMRSYLEDELPSLVLRNFASADLTRQGITGHSMGGHGALTI
                     GLRGQDRFRSVSAFSPIVAPSQCPWGEKALTGYLGDDREGWHAYDACALLDQGLRVPD
                     LLVDQGEADNFLTEQLRTELLVDACERAGQKAEIRMQPGYDHSYYFISTFMAEHVAWH
                     AERLKA"
     gene            complement(5147..>5645)
                     /locus_tag="VN034_11875"
     CDS             complement(5147..>5645)
                     /locus_tag="VN034_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWV61349.1"
                     /translation="IGATVIGTVSTEAKAHAAREAGADHVLMYKTDDVAAHVREITDG
                     QGVPVTFDGIGMATWATSLKATARRGLIVSYGNAGGPVTGVNLGILAQHGSQFVTRPT
                     LFDYYLHPGERAAGAARVFEMIESGAVRITVGQRYSLEDAARAHADLEAGRTTGSTLL
                     IPEHN"
BASE COUNT          988 a         1817 c         1869 g          971 t
ORIGIN      
        1 gatcgacgcc agccgcatcg cgatgatcgg cgacggcacg catgtcgtca gcctcgacac
       61 ggtgatcgcg acgatgctgc aaaccggccg cgacatgcgc gacaaatata aggaaacgtc
      121 gaagggcggg ctggcggtca gcgtggtgga atgttgagga aataatttcg gaaggtcgcg
      181 tgacaggcgg ctttggcaag cttaatatcg gaatagtcga aaatagagca gcagggtggt
      241 ggctcctgcg cctgcatcgc gggttcttct cagcctcggc gtcaactggc gtggttcggt
      301 gtggcgcgtt gaggaattgc gcttcgccca cccgcatagc ggagcgtgac atgaactatt
      361 ttcttcttgg tattgccatc atcgccgaag tcgtaggcac ctccttcatg aaacagtcgg
      421 agggcttcac caaactggcg cccagcctcg tgacggtcac cgcttatgtg gtcgccttct
      481 attgcctgtc gctgacgcta aagacgattc cgacgggcgt ggcctatgcc atctggtcgg
      541 gtgtcgggat cgttctcatc gccgcggttg catggatttt ccagggtcaa aggctcgatg
      601 caccggcgat gctcggcatg gggctcatca tctcgggcgt ggtcgtgatg aacctgtttt
      661 caaaagtcgc tgcgcattag gcccaaatat ttgcctttgc aggcagcggt gcggtggcga
      721 tcacgcgctt gcattggcgc ggcgtttgcc tgattgggcg tgcgagaagg agaccgccca
      781 tgaagacccg cgccgccgtt gcgttcgaag cgaagaagcc gctcgagatc gtcgagctcg
      841 acctcgaagg cccgaaggcg ggcgaagtgc tggtcgagat catggcgacg ggaatctgtc
      901 acaccgacgc ctatacgctc gacggcttcg acagcgaggg catcttcccg agcgtgctgg
      961 gacatgaggg cgcgggcatc gtgcgcgagg tcggcgcggg cgtcaccagc gtgaagccgg
     1021 gcgaccatgt catcccgctc tacacccccg aatgccgcca gtgcaaaagc tgcctttcgg
     1081 gcaagaccaa tctttgcacc gcgatccgcg ccacccaagg caaggggctg atgcccgacg
     1141 gcacgacgcg cttttcgtac aagggccagc cgatcttcca ttatatgggc tgctcgacct
     1201 tctcgaactt caccgtgctg cccgagatcg cggtcgcgaa gatccgcgag gacgcgccgt
     1261 tccagtcgag ctgctacatc ggttgcggcg tcaccaccgg cgtcggcgcg gtgatcaaca
     1321 cggcgaaggt gcaagtcggc gacaatgtcg ttgtcttcgg gctcggcggg atcgggctca
     1381 acgtgatcca gggcgcgcgg ctcgccggcg ccgacaagat catcggcgtc gacatcaacc
     1441 ccgatcgcga ggaatggggc cgcaagttcg gcatgaccga cttcctcaac tcgaagggca
     1501 tgagccgcga agacacggtc gcggcgatcg tcgcgatgac cgacggcggc gccgactaca
     1561 ccttcgacgc gacgggcaat accgaagtga tgcgcattgc cttggaagcg tgccaccgcg
     1621 gctggggcac ctcgatcatc atcggcgtcg ccgaggcggg caaggagatc gcgacgcgcc
     1681 cgttccagct cgtcaccggg cgcaactggc gcggcaccgc gttcggcggc gccaaggggc
     1741 gcaccgacgt gcccaagatc gtcgacatgt atatgaccgg caagatcgag atcgacccga
     1801 tgatcaccca tgtcatgggg ctcgaggaga tcaacaaggg cttcgacctg atgcacgcgg
     1861 gggaaagcat ccgcagcgtc gtcgtctact aagggaaaac aacgcatgtt cagtcatgtg
     1921 gcgctggggt cggacgacat cgcggcgagc aaacggttct acgacgcggt tctcggcgcg
     1981 ttcggcgcgc ccgagggcgt gatcgacgag cgcggacggc tggtctacat gcacgccggc
     2041 ggccgcctga tcgtgacgca cccggtcaac ggcgaaatcg ccgcatgcgg caacgggcat
     2101 acgctggggt tcctcgcgcc ctcgaccgac gcggtcgatg cctggcacgc cgcgggggtc
     2161 gcgaacggcg ggacgacggc ggagaatccg ccgggcatcc gccatgccac cgaggggcgc
     2221 accgtctatc tcgcctatct gcgcgatccg gccggcaaca agctctgcgc cacgcacaag
     2281 atttcgggct gagatggggg acgatcgcgc gcccgcttat gtgctgacct tcagctgcgt
     2341 cgacgcggtc gggatcgtcg cggcggtgac ggggctgctc gcggagcgcg acggcttcat
     2401 cctcgacagc cagcaatatg ccgacctcga ttcggggcgc ttcttcatgc gcgtcgagtt
     2461 tcgcggcgaa gggccgcgct ttcccgaagg actggcgggc gtgcaggacg ccttcgcgcc
     2521 ggtcatcgcg cgcttcgcga tggacgcgcg gatcagcgat accgccgcca agccgcgctt
     2581 cgtcatcgcg gtgtcgcagg gcagccattg cctcaacgac ctgctccacc gctggtcgac
     2641 gggcaatctc gcgatcgacg tcgtcggcgt cgtgtcgaat cacgaggcgc agcggcggct
     2701 ttccgaatgg cacggcgtac cgttccatta tctgccagtg agcgatgcga accgcgcgga
     2761 ccaggaagcg gcgatcctcg acgtcatggc gcgcggcggc gcggaatatc tggtgctcgc
     2821 gcgctatatg caggtgctct cgtccgacct gtcggcacgg ctcgccggcc gctgcatcaa
     2881 catccaccac agcttcctgc ccggcttcaa gggcgccaag ccctatcacc gcgcgcagga
     2941 gcgcggggtc aagctgatcg gcgcgaccgc gcatttcgtg acgagcgacc tcgacgaggg
     3001 gccgatcatc gaacaggcgg tcgagcgcgt cgatcaccgc gacggcgtcg acgagctgat
     3061 ccgcatcggc cgcgataccg aggcgcaggt gctggcgcgc gccgtgcgct gggtcgccga
     3121 gcagcgggtt ttgatcgacg gccgcaagac ggttgttttc cgctagcact ttcgggttcg
     3181 tcattttcgg ggaacccgcg aacatgatgg cggctggtcc tgaccggcgg ggcagggtag
     3241 gtatcgaatc gcaaccagaa tcaggaggag cgctaaccca tgtacagtca caatatggtc
     3301 ggatcgaacg acatcgaggc gtcgaagaaa ttctatgacg cgacctttca ggcgatcggc
     3361 ggcaagccgg cgattgtcga tgacaaggga cgtctgatct atatccacaa tggcgggctg
     3421 ttcctcgtcg gcgcccccat cgacggcgag cccgcgacca tcggcaatgg ctgcacgatc
     3481 ggctttgcga tggacggccc cgaacaggcc gacgcatggc acaaggcggg cgtcgaaaat
     3541 ggcggcacct cgatcgaaga cccgccgggc gtgcgcgaag gcggtttcgg cgcgatgtat
     3601 ctcgcctatc tgcgcgatcc gtcgggcaac aagctctgcg cgcttcaccg cgtcgtctga
     3661 gaccggaaga ggaatagtca tgcgcggcga agcgggattg ctggcggacc ttggcgcgct
     3721 cgccatcccc ttcgccgccc atgagcatga agcggtgttc accgttgcag agagcgatgc
     3781 ggtcaacgcc gccataccgg gggcgcatac caagaatctg ttcctcaagg acaatggcgg
     3841 cgcctattgg ctcgtcaccg tgcccggcgc ggcgcgcgtc gacctgaagg ccctgcccgc
     3901 cgcgatcggc agcaagcggg tgagcttcgg caaggccgag gacatggaac ggctgctcgg
     3961 catttcgccg ggatcggtga cgccgctcgc cgcgatcaac gctccgccgg gaagcatcac
     4021 cgtcgtgctc gacgaggggc tggccgcggc ggagacggtg aacgtccatc cgctccgcaa
     4081 taccgcgacg cttgggctgc cgggcgcaac gatcctcgac ctcttgcgtc attggggaca
     4141 tcgaccgctc gtcgcctcca tccccgttca ggaccccgca tgaccatcga aaccctttcc
     4201 accgttcgca gccatggcgg cacgcaaggc gtctacaagc atgcgagcac gaccaccggc
     4261 accgacatga ccttcgcggt cttcgtcccc gaccatgaag gcggcgcgaa gctgccggtg
     4321 ctctggtatc tgtcggggct gacctgcacc catgccaatg tgatggagaa gggcgagtat
     4381 cgcgccgcct gcgccgaaca tggcgtgatc ttcatcgccc ccgataccag cccgcgcggc
     4441 gaaaatgtcc ccgacgatcc cgacgccgcc tatgatttcg ggctcggcgc gggcttctat
     4501 gtcgacgcga ccgccgaacc ctgggcgaaa cattaccgga tgcgctcgta ccttgaggac
     4561 gagctgccgt cgctcgtgct gcgcaatttc gcgagcgccg acctgacgcg gcaggggatc
     4621 accggccatt cgatgggcgg gcatggcgcg ctgaccatcg ggcttcgcgg tcaggaccgc
     4681 ttccgctcgg tgtcggcctt ttcgccgatt gttgcgccat cgcaatgccc gtggggcgaa
     4741 aaggcgctga cgggctacct cggcgacgac cgcgagggct ggcacgccta tgacgcgtgc
     4801 gcgctgctcg atcaggggct gcgcgtgccc gacctgctcg tcgatcaggg cgaggccgac
     4861 aatttcctga ccgagcagct caggaccgag ctgctcgtcg acgcctgcga gcgcgcgggt
     4921 cagaaggccg aaatccggat gcagccgggc tatgatcaca gctattattt catctcgacc
     4981 ttcatggccg agcatgtcgc gtggcacgcg gaacggttga aggcttgatc gctcccctcc
     5041 cttcagggag gggctgggcg agggcatgtg acgtcgccaa ccctccccgc tgcgactagc
     5101 gagcaagctc gcaagtctcg ccgcccctcc cgcaagcggg agggggctag ttgtgctccg
     5161 gaatcagcaa cgtcgacccc gtcgtgcgtc ccgcctcaag gtcggcgtgc gccctggccg
     5221 catcctccag cgaataacgc tgccctacgg taatcctcac cgcgccgctt tcgatcatct
     5281 cgaacacgcg cgcggcgccg gcggcgcgtt cgccggggtg gagataatag tcgaacagcg
     5341 tcggccgcgt cacgaactgc gacccatgct gcgcgaggat gccgagattg acgccggtga
     5401 ccggcccgcc ggcgttgcca tagctgacga tcagcccgcg ccgcgcggtc gccttgagcg
     5461 acgtggccca tgtcgccatg ccgatgccgt cgaacgtgac ggggacgccc tggccatcgg
     5521 tgatttcgcg gacgtgcgcg gcgacatcgt cggtcttgta catcaggaca tggtcggcgc
     5581 ccgcttcgcg cgcggcatgc gccttggctt cggtgctcac cgtgccgatc acggtcgcgc
     5641 cgatc
//
LOCUS       DATMSU010000443         4335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_8011, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000443 DATMSU010000000
VERSION     DATMSU010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8011"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VN034_11880"
     CDS             complement(<1..218)
                     /locus_tag="VN034_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HWV61350.1"
                     /translation="MDTQTLVRLLDLVGIGVFALSGALMAVRLRQTLVTAAFFALVTG
                     VGGGSVRDLLIGAPVFWVQDGAIAAVCIA"
     gene            272..733
                     /locus_tag="VN034_11885"
     CDS             272..733
                     /locus_tag="VN034_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV61351.1"
                     /translation="MEGRCTCGAVRYRMTAAPMIVHCCHCRWCQRETGSAFVINAVIE
                     SDRIEAEGALDHVMTPSESGKGQEIIRCPACRVALWSHYPTAGRRSSFVRVGTLDDPD
                     RYPPDVHIFTRSKQPWVILPADAKVFPDFYPSPEGVWSDDARLRWQRMMVG"
     gene            complement(736..2073)
                     /locus_tag="VN034_11890"
     CDS             complement(736..2073)
                     /locus_tag="VN034_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV61352.1"
                     /translation="MAKHGLWAAASLTLSLMATSAQAAPDLGAANARLTALLDKNYQA
                     LETLYKDLHQHPELGMQEVRTAGILAKHLRSAGFTVTEKVGGTGVVGVLKNGEGPTIL
                     VRADMDALPMEEKTGLAWASKARATYEGKDVPVMHACGHDTHVAYLVGVAQALSAMRD
                     SWSGTVVLIGQPAEEPLSGARAMLDDGLFKRFPKPDFGFAAHVTNLPAGVVAIKAGPS
                     SSASDSYAITFHGRGGHGSMPAATIDPIPIAARFVTDTQVVISREKDPAAFGVLTIGA
                     INAGSAPNIIPDSAEVKVNLRSQSPEVRKLLKDGTARVAKSAAAMGGAPEPTIRYLGG
                     TGIMMNDSAMAKAAVAALTPTFGKGLVFAPESATPMSGSEDYSEFVDAGVPSLFFGIG
                     GYDPTVLAGLKAKGEPAPTNHSPFFAPKAEPAIRNAVTAIVLSIVGGVRPAAK"
     gene            complement(2099..3496)
                     /gene="lpdA"
                     /locus_tag="VN034_11895"
     CDS             complement(2099..3496)
                     /gene="lpdA"
                     /locus_tag="VN034_11895"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWV61353.1"
                     /translation="MADYDYDVLVIGAGPGGYVAAIRAAQLGLKTACAEGRETLGGTC
                     LNVGCIPSKAMLHASECFEAAAGGAMAAMGIKVKPELDLPAMHGQRKDAVKGLTGGIE
                     FLFKKNKIDWLKGYAQFTSKDTVEVAGKAVRAKNIIIATGSSVTPLPGVEVDNDKQII
                     VDSTGALELAKVPGHMVVIGGGVIGLELGSVWRRLGAKVTVVEFLDQILPGMDADVRK
                     EANKILKKQGMEFKLKTKVTKAAVKGKKAVLTLEPAAGGDAETLEADVVLVSIGRRPN
                     TDGLALDKAGLAVNQRGQIETDHDFSTQVPGIWAIGDVIPGPMLAHKAEDEGIACAEN
                     IAGLTGIVNHDVIPSVVYTWPEIAGVGLTEEQAKEKGEVKVGKFPMMANSRAKTNHEP
                     DGFVKVIADAKSDRVLGVWCIASVAGTMIAQAAQAMEFGATSEDIAYTCHAHPTHSEA
                     IKEAAMAVTGKPIHI"
     gene            complement(3596..>4335)
                     /gene="sucB"
                     /locus_tag="VN034_11900"
     CDS             complement(3596..>4335)
                     /gene="sucB"
                     /locus_tag="VN034_11900"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HWV61354.1"
                     /translation="AAAPSAPAAAAPGRQEERVKMTRMRQTIAKRLKAAQDTAAMLTT
                     FNDVDMSAVMATREKYRDSFEKKHGVRLGFMSFFTKAVALAAHDIPAVNARIDGDEIV
                     YHNYLDVSVAVSAPNGLVVPVVRNADSMSFADIEKAIADLGKRAKDGTLTMDDMTGGT
                     FTISNGGVFGGLMSTPIINPPQSAVLGLHRIEDRPVVRDGEIVIRPMMYLAMSYDHRL
                     IDGREAVTFLKTIKEAIEDPTRLLIDL"
BASE COUNT          691 a         1444 c         1417 g          783 t
ORIGIN      
        1 gcgatgcaca ccgcggcgat cgcgccatcc tggacccaga acaccggcgc gccgatcaac
       61 aggtcgcgca cgctgccgcc accgacgccg gtgacgagcg cgaagaaggc ggcagtcacg
      121 agcgtctggc gcagccgcac cgccatcagc gcgcccgaca gcgcgaagac gccgatgccg
      181 acaaggtcga gcaggcggac gagcgtttgt gtatccatcg cgcgcgggac tatcatcgcg
      241 cgcgaaaacg cacaatggag aggagcaagc catggaaggc cgatgcactt gcggggcggt
      301 tcgttaccgg atgaccgcgg cgccgatgat cgtccactgc tgtcattgcc gctggtgcca
      361 gcgcgagacc ggcagcgcct ttgtgatcaa cgcggtgatc gagagcgacc ggatcgaggc
      421 cgaaggagcg ctcgaccatg tgatgacccc ctccgaaagc ggcaaggggc aggaaatcat
      481 ccgctgcccg gcgtgccggg tcgcgctgtg gagccattat cccaccgcgg gccgccgttc
      541 ctccttcgtc cgcgtcggga cgctcgacga ccccgaccga tacccgcccg acgtgcatat
      601 cttcacgcgc agcaagcagc cgtgggtcat tctgcccgcc gatgcgaaag tctttccgga
      661 tttctacccg tcgcccgaag gcgtgtggag cgacgacgcg cgacttcggt ggcagaggat
      721 gatggtcggc tgagcctatt tcgcagcggg ccgcacgccc ccgacgatcg aaaggacgat
      781 cgcggtcacg gcattgcgga tcgccggttc ggccttcggc gcgaagaaag gcgagtggtt
      841 cgtcggcgcg ggttcgccct tcgccttcaa cccggcgagc acggtagggt cgtagccgcc
      901 gatgccgaag aagagcgacg gcacgcccgc atcgacgaat tcggagtaat cttcgctccc
      961 cgacatcggc gtcgcgcttt cgggagcgaa gacgagcccc ttgccgaacg tgggcgtcag
     1021 cgccgcgacc gcggctttgg ccatcgccga gtcattcatc atgatgccgg tgccgccgag
     1081 gtagcggatc gtcggttcgg gcgcgccgcc catcgcggcc gccgatttcg cgacacgcgc
     1141 ggttccgtcc ttgagcagct tgcgcacctc gggcgattgc gagcgcagat tgaccttcac
     1201 ctcggcgctg tcggggatga tgttgggcgc gctgccggcg ttgatcgcgc cgatcgtcag
     1261 cacgccgaaa gcggccggat ccttctcgcg gctgatcacc acctgcgtgt cggtgacgaa
     1321 gcgcgcggcg atcggaatcg ggtcgatcgt cgccgcgggc atcgagccgt ggccgccgcg
     1381 gccgtgaaag gtgatcgcat agctgtccga agccgacgag gacggtcccg ccttgatcgc
     1441 gacgacgccg gccggaaggt tggtgacgtg cgcggcgaag ccgaaatcgg gcttggggaa
     1501 gcgtttgaag agcccgtcgt cgagcatcgc gcgcgcgccg ctgagcggct cttcggcggg
     1561 ctggccgatc agcaccacgg tgcccgacca gctgtcgcgc atcgcgctca aggcctgcgc
     1621 gacgccgacc agataggcga catgcgtgtc gtggccgcac gcgtgcatga cgggcacatc
     1681 cttaccctca taggtcgcgc gcgccttgct cgcccaggcg agcccggtct tttcctccat
     1741 cggcagcgcg tccatgtcgg cgcgcacgag gatggtcggc ccctcgccat tcttgagcac
     1801 gccgacgacg ccggtgccgc cgaccttctc ggtcacggtg aagcccgcgc tgcgcagatg
     1861 ctttgcgaga atgcccgcgg tgcggacctc ctgcatcccg agttcggggt gctggtgcag
     1921 atccttgtag agcgtttcaa gcgcctgata atttttgtcg agcagcgcgg tcagccgcgc
     1981 gttggcggcg ccgagatcgg gcgcggcctg cgcgctggtt gccatgaggg agagggtcag
     2041 cgaagccgct gcccagagcc cgtgcttcgc catcatactc tcccccaatt tatccgtgtc
     2101 agatgtggat cggcttgccc gtcaccgcca ttgcggcttc cttgatcgcc tcgctgtgcg
     2161 tcggatgcgc gtggcaggta taggcgatgt cttccgacgt cgcgccgaat tccatcgcct
     2221 gcgccgcctg cgcgatcatc gtgcccgcga cgctcgcgat gcaccagacg ccaagcacac
     2281 ggtcgctctt ggcgtcggcg atcaccttca cgaaaccatc gggctcgtgg ttggtcttgg
     2341 cgcggctgtt cgccatcatc gggaacttgc cgacctttac ctcgcccttt tccttcgcct
     2401 gctcttcggt caggccgacg ccggcgattt cgggccaagt gtagacgacc gacgggatga
     2461 cgtcgtggtt cacaatgccg gtcagccccg cgatattctc ggcgcaggcg atgccttcgt
     2521 cctcggcctt gtgcgcgagc atcgggccgg ggatgacgtc accgatcgcc cagatgccgg
     2581 gaacctgcgt cgaaaaatca tggtcggttt cgatctgacc gcgctggttc accgcgagac
     2641 cggccttgtc gagcgccaag ccgtcggtgt tcggacggcg gccgatcgac acgagcacga
     2701 catcggcttc gagcgtctcg gcgtcgccgc cggccgcggg ttcgagcgtc agcacggcct
     2761 tcttgccctt gaccgcggcc ttggtgacct tggtcttgag cttgaattcc atgccctgct
     2821 tcttgaggat cttgttcgcc tccttgcgga cgtccgcgtc catgccgggc agaatctggt
     2881 cgaggaactc gacgaccgtg accttcgcgc cgagacggcg ccacacgctg ccgagttcga
     2941 gcccgatcac gccgccgccg atcacgacca tatgacccgg caccttcgcg agttcgagcg
     3001 cgccggtcga atcgacgatg atctgcttgt cattatcgac ctcgacgccc ggaagcgggg
     3061 tcaccgacga gcccgtcgcg atgatgatgt tctttgcacg gacggccttg cccgcgactt
     3121 ccaccgtgtc tttcgaggtg aactgggcat agcctttcag ccagtcgatc ttgttcttct
     3181 tgaacaggaa ctcgatgccg ccggtcaggc ctttcaccgc atccttgcgc tggccatgca
     3241 tcgcggggag gtcgagctcg ggctttacct tgatacccat cgccgccatc gcgccgcccg
     3301 ccgccgcctc gaaacattcg gacgcgtgca gcatcgcctt cgacgggatg cagccgacgt
     3361 tgaggcaggt gccgcccagc gtctcgcgcc cttcggcgca cgcggttttg aggcccagtt
     3421 gcgccgcgcg gatcgccgcg acataaccgc cgggaccggc accgatgaca aggacgtcgt
     3481 aatcgtaatc agccataact cactccattc gtcatcccag cgaaagctgg gatcgctggc
     3541 tgccaaaggc ggcgccaggg ggatagcggc cccagcattc gctggggcga cggaactaca
     3601 ggtcgatcaa caaccgcgtc ggatcctcga tcgcttcctt gatcgtcttg aggaaggtca
     3661 ccgcctcgcg gccgtcgatc aggcggtggt catagctcat cgcgagatac atcatcggac
     3721 ggatcacgat ttcgccgtcg cgcacgaccg ggcgatcctc gatgcggtgg aggccgagca
     3781 ccgccgactg cggcgggttg atgatcgggg tcgacatcag gccgccgaac accccgccgt
     3841 tcgagatggt gaaggtgccg ccggtcatgt cgtccatcgt cagcgtgccg tccttggcgc
     3901 gcttgccgag gtcggcgatc gccttttcga tgtcggcaaa cgacatgcta tccgcgttgc
     3961 ggacgacggg cacgacgagg ccgttcggcg cgcttaccgc gaccgagacg tcgagataat
     4021 tgtgatagac gatctcgtcg ccgtcgatcc gcgcgttgac cgccggaata tcatgcgcgg
     4081 cgagcgcgac cgccttggtg aagaagctca tgaagccgag gcgcacgcca tgcttctttt
     4141 cgaagctgtc gcgatatttc tcgcgcgtcg ccatcaccgc cgacatgtcg acgtcgttga
     4201 aggtcgtcag catcgccgcg gtgtcctgcg ccgccttgag gcgtttcgcg atcgtctggc
     4261 gcatgcgcgt catcttgacg cgctcttcct gacggccggg cgcggcggcg gcaggcgccg
     4321 acggggcggc agcgg
//
LOCUS       DATMSU010000444         7265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_159220,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000444 DATMSU010000000
VERSION     DATMSU010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159220"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VN034_11905"
     CDS             <1..396
                     /locus_tag="VN034_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HWV61355.1"
                     /translation="AAPGCAVTAAMALWDRDVVQPAAERYFGQKVVEVENLGSYNCRK
                     IAGREAQSEHSTANAIDISAFVLADGTRISLINDWAPGDQRSEFLHDVRDGACDLFST
                     VLSPDHNAAHANHFHLDMAARTAGWSVCR"
     gene            complement(393..1127)
                     /locus_tag="VN034_11910"
     CDS             complement(393..1127)
                     /locus_tag="VN034_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWV61356.1"
                     /translation="MVRAFYLAYAAVCYAIFFATFLYLIAFVGNFSFVQPTVDFAREA
                     PPGRTLLIDVALIALFGVQHSAMARPGFKAAWTRIVPAPIERSTYVLLASLALIILML
                     FWHEWATPVWTVSNPVGAAILWALFALGWVIVLVSTFLINHFELFGLQQAWFALRERA
                     AAPPVLRQPFFYRFVRHPLYAGFFIAFWATPIMTAGHLLLAAGFSLYMLIAIRLEERD
                     LIDVFGEAYAGYRRRVGMLVPRVGRR"
     gene            complement(1187..1543)
                     /locus_tag="VN034_11915"
     CDS             complement(1187..1543)
                     /locus_tag="VN034_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HWV61357.1"
                     /translation="MTETKARTRTAAVTLTPNAEARIAKLMASAPEGAIGVRLSTPRR
                     GCSGLAYSVDYVTEEVKFDEKIETPGGVFYIDGASVLYLIGSVMDWVEDDFAAGFVFE
                     NPNAKGACGCGESFTV"
     gene            complement(1543..2013)
                     /locus_tag="VN034_11920"
     CDS             complement(1543..2013)
                     /locus_tag="VN034_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HWV61358.1"
                     /translation="MSENGTIKVEEVESVEAPPKARVEDVETAPEKLERKRDYLEGFL
                     AAKPAAVEPGAVGGDLYEAVIDALKEIFDPEIPVNIYDLGLIYNVEIDEGHVLVTMTL
                     TTPHCPVAESMPAEVELRVGAVPGVGDAEVNLVWDPPWSPQNMSDEARLELGML"
     gene            complement(2010..3212)
                     /locus_tag="VN034_11925"
     CDS             complement(2010..3212)
                     /locus_tag="VN034_11925"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HWV61359.1"
                     /translation="MPNGNARTPGVSTALDTNGIRSQFPGLTPGWHYLDTAATAQKPQ
                     AVIDAMVNAMGRDYATVHRGVYARSADMTLAYEAARRRIAAFIGARENDVTFVRGATE
                     AINLVAYSHPKKGRVLLSLLEHHSNIVPWQLAGWQVDVCPLTEDGCIDLDAAEKLLTP
                     EHTMVAFAHVSNVLGSPLDVTRAAELAHRVGAELLVDGCQAVPRLPVDVAALGCDYYV
                     FSGHKLYGPTGIGVLWSDKLSALPPWQGGGSMIDRVTFEKTTYAPAPTRFEAGTPAIV
                     EAIGLAAAVDYVESLGIDRVHAHECALVASLREALARKNSITLYGPENSAGIVSFSMA
                     GVHPHDIGTILDESGVAIRAGHHCAQPLMEHLGVPATARASFGVYNNDDDVAALIDGL
                     ARVERIFG"
     gene            complement(3251..3982)
                     /locus_tag="VN034_11930"
     CDS             complement(3251..3982)
                     /locus_tag="VN034_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HWV61360.1"
                     /translation="MKTAALPSRRDEAWRYADIDAVTAAWPLPAPESIIVPAGGDFKR
                     AIIQNEGAIQQIEMSIGKGATASLHILNIGGAYGRIELAVTLHEDADFTLGAAQIGGG
                     EQTLEIITTVTHAEPGATSRQMVRSVLGGQATGTYLGKVAVARDAQQTDSEQDVKAML
                     LERSATANAKPELEIFADDVKCAHGCAIGELDAMALYYLQSRGLPPAEAKKLLLQAFV
                     AGVFDGAEDEARLQELALAKLGELV"
     gene            complement(3979..4809)
                     /gene="sufC"
                     /locus_tag="VN034_11935"
     CDS             complement(3979..4809)
                     /gene="sufC"
                     /locus_tag="VN034_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWV61361.1"
                     /translation="MLKIENLHAAVADKPILKGLSLAINAGEIHAIMGPNGAGKSTLS
                     YVLGGRPGYDVTEGSVSFTPDVIPGESQGPDEAIELGPDFRRGTGTTETLDLLALDPH
                     ERAAAGLFLGFQYPVEIPGVSNVQFLRESLNAQRKARGQEPLSGGDFLKLAREKAGLL
                     KLDMDMLKRPVNVGFSGGEKKRNEMVQMGILDPRLAILDETDSGLDIDALRIVGDGIN
                     SIMRRPDKAVLLITHYQRLLDYVQPDFVHVLAGGRIVKSGGPELARELEAHGYAEMMA
                     "
     gene            complement(4812..5411)
                     /locus_tag="VN034_11940"
     CDS             complement(4812..5411)
                     /locus_tag="VN034_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61362.1"
                     /translation="MPINQLTRSFALAGVALLATAGMSSAVQASPSYLKAANIPFALA
                     YGTCVYADSPAADCTTVRQSLLAEADPILNRFHFSDGLETRRSLRVLFDNIDRQAVKL
                     REEDKTLAPETLSFMSCTADAIRDQNDFQRGVFISGSDAFKACEDIYDAYLARDRSSA
                     TVQRNVNFMRHIKDMLPNSPVDGQRLRREGLLTERGPRG"
     gene            complement(5453..6940)
                     /gene="sufB"
                     /locus_tag="VN034_11945"
     CDS             complement(5453..6940)
                     /gene="sufB"
                     /locus_tag="VN034_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWV61363.1"
                     /translation="MTDNTELPVKDQAAHDAAAKVADYEHGWSSTIETDFAPKGLTED
                     TVRFISAKKNEPEWMLDWRLKAFRHWQTMETPDWAKLNVPPIDYQDAYYYAAPKAKPK
                     LSSLDEVDPEILEVYKKLGIPIEEQKVLAGVEGARKVAVDAVFDSVSVATTFREELKR
                     AGVIFLSISEAIREYPELVKKWLGKVVPMHDNYFATLNCAVFSDGTFVYVPEGVRCPM
                     ELSTYFRINAENTGQFERTLIVADKGAYVSYLEGCTAPMRDENQLHAAVVELVALDDA
                     EIKYSTVQNWYPGNAEGLGGIYNFVTKRALCQGKRSKVSWTQVETGSAITWKYPSCVL
                     NGDDSVGEFYSVAVTNNYQQADTGTKMIHNGKRTRSTIVSKGISAGKSSGTYRGLVRV
                     APNAEGVRNFTQCDSLLLGDQCGAHTVPYIEVRNPSATVEHEATTSKISDDQLFYAMQ
                     RGLGQEEAVALIVNGFAKEVLQQLPMEFAVEAQKLLGISLEGSVG"
     gene            complement(6946..>7265)
                     /locus_tag="VN034_11950"
     CDS             complement(6946..>7265)
                     /locus_tag="VN034_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HWV61364.1"
                     /translation="LARPAAAINVADIIEAVEGPIALTACVAEGRHDCSLEGSCKVQP
                     HWGVVNGAVRGALAGISLASLSVAPAKGAGSHMPPDTAAGESMPAAPSKTIAAPAFAG
                     ATS"
BASE COUNT         1262 a         2338 c         2328 g         1337 t
ORIGIN      
        1 gcggcgcccg gctgcgcggt gaccgcggcg atggcgctat gggaccgcga cgtcgtccag
       61 ccggcggccg agcgatattt cgggcagaag gtcgtcgagg tcgaaaatct cggcagctat
      121 aactgccgca agatcgccgg gcgcgaagcg cagagcgagc attcgaccgc caatgcgatc
      181 gacatttcgg cctttgtcct cgccgacggg acgcgcattt cgctgatcaa cgactgggcg
      241 cccggcgacc agcgcagtga attcctccac gatgtccgcg acggggcgtg cgacctgttc
      301 agcacggtgc tgtcacccga ccataatgcg gcgcacgcca atcatttcca tctcgacatg
      361 gcggcgcgca ctgcgggctg gtcggtctgc cgctagcggc ggccgacgcg cggaacgagc
      421 atgccgacgc gcctgcgata gccggcatag gcctcgccga agacgtcgat caggtcgcgt
      481 tcttcgagcc ggatcgcgat cagcatatag agcgaaaagc cggccgcgag cagcagatgc
      541 cccgcagtca tgatgggcgt cgcccagaaa gcgatgaaaa atccggcata gagcgggtgg
      601 cggacgaacc tgtaaaagaa cggctgccgt agcaccggcg gcgccgcggc ccgctcgcgc
      661 aacgcgaacc acgcctgttg cagcccgaac agctcgaaat ggttgatcag gaaggtgctg
      721 acgagaacga tcacccagcc gagcgcgaag agggcccaca ggatggcggc gccgacggga
      781 ttgctcaccg tccagaccgg cgtcgcccat tcgtgccaga acagcatcaa tatgatcagc
      841 gccaggctgg cgagtaacac ataggtgctg cgctcgatcg gcgcgggcac gatgcgcgtc
      901 cacgccgcct tgaaccccgg ccgcgccatc gcgctgtgct ggacaccgaa cagcgcgatc
      961 agcgcgacgt cgatcagcag cgttcgcccg ggtggcgcct cgcgcgcgaa atcgacggtc
     1021 ggctggacga aggaaaagtt gccgacgaac gcgataagat aaagaaatgt cgcgaagaat
     1081 atcgcgtaac agacggccgc ataggccaga tagaaggcac gaaccatggt gctgcccctt
     1141 cttttggagg agccatcata gcatgttttt cgcggaaaag cgcgctttag acggtaaagc
     1201 tttcgccgca gccgcacgcg cccttggcgt tcggattctc gaagacgaag cccgcggcga
     1261 agtcgtcctc gacccagtcc atgacactgc cgatcaggta cagcaccgac gcgccatcga
     1321 tgtagaagac gccgccgggg gtctcgatct tttcgtcgaa cttcacctct tcggtcacat
     1381 agtccaccga ataggcgagt cccgagcaac cgcggcgcgg ggtcgacagg cggacgccga
     1441 tcgcgccttc gggcgccgac gccatcagct tggcgatgcg cgcctcggca ttcggcgtca
     1501 gggtgacggc ggccgtgcga gtgcgggctt tggtctcggt catcacaaca tccccagttc
     1561 gaggcgtgcc tcgtcgctca tattctgcgg cgaccagggc ggatcccaga cgagattgac
     1621 ctcggcatcg ccaaccccgg gcaccgcgcc gacgcgcagt tcgacctcgg cgggcatcga
     1681 ctcggcgacg gggcagtgcg gcgtcgtcag cgtcatcgtg acgagcacat gcccctcgtc
     1741 gatctcgaca ttgtagatga ggccgagatc atagatgttt accggaattt ccgggtcgaa
     1801 aatttccttg agcgcgtcga taacggcttc gtagaggtcg ccgccgaccg cgccgggttc
     1861 gaccgccgcc ggcttggcgg cgaggaaacc ttccagatag tcgcgcttgc gctcaagctt
     1921 ctcgggcgcc gtctcgacgt cctcgacgcg cgctttcggc ggcgcttcga cgctttccac
     1981 ttcctcaacc ttgatcgtgc cgttctcgct catccgaaaa tcctctcgac gcgggcgagg
     2041 ccatcgatca gcgccgccac atcgtcgtca ttgttgtaaa caccaaaact ggcgcgcgcg
     2101 gtcgcgggca cccccagatg ctccatcagc ggctgtgcgc aatggtgtcc ggcgcggatc
     2161 gcgaccccgc tttcgtccaa gatagtgccg atgtcgtgcg gatgaacccc cgccatcgaa
     2221 aaactcacga ttccggcact gttttcaggg ccatagagcg tgatactatt cttgcgtgcg
     2281 agggcttcgc gcaagctggc gaccagtgcg cattcgtgcg cgtggaccct gtcgatgccc
     2341 agtgactcga cataatcgac cgccgcggcg agcccgatcg cttcgacgat tgccggcgtg
     2401 ccggcctcga agcgcgtggg ggcgggggcg taggtggttt tttcgaaggt cacgcggtcg
     2461 atcatcgacc cgccgccctg ccacggcggc agcgcgctga gcttgtcgct ccagagcacg
     2521 ccgatgcccg tcgggccgta aagcttgtgc cccgaaaaga cataatagtc gcagccgagc
     2581 gccgctacgt cgacgggcag gcgcgggacg gcctggcagc cgtcgaccag cagctcggcg
     2641 ccgacgcggt gcgccagttc ggcggcgcgc gtgacgtcga gcgggctgcc gagcacgttc
     2701 gagacatgcg cgaaggcgac catcgtgtgc tcgggcgtga gtagcttctc cgcggcgtcg
     2761 aggtcgatgc agccgtcttc ggtcaacggg cacacgtcga cctgccagcc ggcaagctgc
     2821 cacggcacga tattgctgtg atgctcgagt agcgacagca gcacgcggcc cttcttcgga
     2881 tgcgaatagg cgacgaggtt gatcgcctcg gtcgcgccgc gcacgaaggt cacgtcattc
     2941 tcgcgcgcgc cgatgaacgc cgcgatccgc cgccgcgccg cttcataagc gagcgtcatg
     3001 tcggccgagc gcgcgtagac gccgcggtgg acggtcgcat agtcgcggcc catcgcattc
     3061 accatcgcgt cgatcaccgc ctgcggcttc tgcgccgtcg cggcggtgtc gagatagtgc
     3121 cagccggggg tgaggccggg aaactggctc cgaattccgt tcgtgtcgag cgcagtcgag
     3181 acaccgggcg tacgtgcatt gccgttgggc atctcgactt cgctcgatgc gaacggggag
     3241 gagaggtcag tcacaccagc tctcccagct tcgcgagcgc cagttcctgc aaccgcgcct
     3301 catcctctgc gccgtcgaat acccccgcga cgaacgcctg gagcagcagc ttcttcgcct
     3361 cggcgggcgg caacccgcgc gactggagat aatagagcgc catggcgtcg agttcgccga
     3421 tcgcgcagcc gtgcgcgcat ttgacgtcgt cggcgaagat ttcgagctcg ggcttggcgt
     3481 tggcggtcgc gctgcgctcg agcagcatcg ccttcacatc ctgctcgctg tcggtctgct
     3541 gcgcgtcgcg cgcgaccgcg accttgccga gataggtgcc ggtggcctgg ccgccgagca
     3601 ccgagcgcac catctgccgc gaggtcgcgc cgggttcggc atgggtgacg gtggtgatga
     3661 tctcgagagt ctgctcgccc ccgccgatct gcgccgcgcc gagggtgaag tcagcgtcct
     3721 cgtgcagcgt caccgcgagt tcgatgcggc cataggcgcc gccgatgttg aggatgtgca
     3781 gcgacgcggt cgcgcccttg ccgatgctca tttcgatctg ctggatcgcg ccttcgttct
     3841 gaatgatcgc gcgtttgaag tcgccgcccg cgggcacgat gatgctctcc ggcgcgggca
     3901 gcggccacgc cgcggttacc gcgtcgatgt cggcgtaacg ccacgcctca tcgcggcgcg
     3961 aggggagggc ggcggttttc aagccatcat ctccgcatag ccatgcgctt ccagttcgcg
     4021 cgccagttcg gggccgcccg acttgacgat gcgcccgccc gcgaggacat ggacgaaatc
     4081 gggctgcaca tagtcgagca gacgctgata gtgggtgatc agcagcaccg ccttgtcggg
     4141 gcggcgcatg atgctgttga tgccgtcgcc gacgatgcgc agcgcgtcga tgtcgaggcc
     4201 gctgtcggtc tcgtcgagga tcgcgagcct ggggtctagg atgcccatct gcaccatctc
     4261 gttgcgcttc ttctcgccgc ccgaaaagcc gacgttcacc ggacgcttca gcatatccat
     4321 gtcgagcttg agcagccccg ccttctcgcg cgcgagcttc aggaaatcgc cgcccgacag
     4381 cggctcttgc ccacgcgcct tacgctgggc gttgaggctt tcgcgcagga actggacgtt
     4441 cgacacgccg ggaatctcga ccggatattg aaagccgagg aacaggccgg cggcggcgcg
     4501 ctcgtgcggg tcgagcgcga ggaggtcgag cgtctcggtc gttcccgtac cccgacgaaa
     4561 gtcggggccc agttcgatcg cctcatctgg accctggctt tcgccgggga tcacgtcggg
     4621 cgtaaagctc accgaacctt cggtaacatc ataaccgggc cgcccgccga ggacgtaaga
     4681 gagcgtcgac ttgccggcgc cattgggccc catgatcgca tggatctcgc ccgcgttgat
     4741 ggcgagcgac agccccttca ggatcggctt gtcggcgacg gcggcgtgaa ggttttcgat
     4801 tttgagcatt atcagcctct tgggccccgt tcggtcagga ggccctctct acgaaggcgc
     4861 tggccgtcca cgggggagtt tggaagcata tctttgatat gccgcatgaa attcacgttt
     4921 cgctgcactg tggcactcga tcggtcgcgg gccagatagg catcataaat gtcttcgcac
     4981 gccttgaagg catcggatcc gctgatgaaa acgcccctct gaaaatcatt ctggtcgcgt
     5041 atcgcgtccg ccgtgcagct catgaaggaa agcgtttcag gagccagcgt tttatcctct
     5101 tcgcggagtt tgacggcctg ccgatcgatg ttgtcgaaca ggacacgcag actccgcctg
     5161 gtttcgaggc cgtccgaaaa atggaaccga ttgagaatgg gatcggcctc tgcgaggagg
     5221 ctttggcgaa ccgtcgtgca gtcggctgcc ggcgagtctg catagacgca ggtgccataa
     5281 gctaacgcga agggaatatt ggccgccttc aaataggatg gagacgcttg gacggcactc
     5341 gacatccccg ccgtcgccaa cagcgcaacg ccagcaagcg caaaagacct ggtcagttga
     5401 ttgataggca taatgagcac ctccactaaa gggggtaaaa tccgcggccg gattacccca
     5461 cgctcccttc aaggctgatc cccaacagct tctgtgcctc gaccgcgaac tccatcggca
     5521 gctgctgcag cacttccttc gcgaagccgt tgacgatcag tgccacggct tcttcctgac
     5581 ccagcccgcg ctgcatcgcg tagaagagct ggtcgtcgct gatcttgctc gtcgtcgctt
     5641 catgctcgac ggtcgcactc gggttgcgga cttcgatata ggggacggtg tgggcgccgc
     5701 actggtcgcc gagaagcagg ctgtcgcact gggtgaagtt gcgcacgccc tcggcattgg
     5761 gcgcgacgcg gacgaggccg cggtaggtgc cgctcgactt gcccgcgctg atccccttcg
     5821 aaacgatggt cgagcgcgtg cgcttgccgt tgtggatcat cttggtgccg gtgtcggcct
     5881 gctgataatt gttggtgact gcgaccgagt aaaattcgcc gacgctgtcg tcgccgttga
     5941 gcacgcagct cggatatttc cacgtgatcg cgctaccggt ttcgacctgc gtccacgaca
     6001 ccttgctgcg cttgccctgg cacagcgccc gcttggtcac gaaattatag atgccgccca
     6061 gcccctcggc attgccggga taccagttct gcaccgtcga atatttgatc tcggcatcgt
     6121 cgagcgcgac cagttcgacc accgccgcat ggagctggtt ctcgtcgcgc atcggcgcgg
     6181 tgcagccttc gaggtaggag acataggcgc ccttgtcggc gacgatcagc gtccgctcga
     6241 actgtcccgt attctcggca ttgatgcgga aataggtgct gagttccatc gggcagcgga
     6301 cgccctcggg cacatagacg aacgtcccgt cggagaagac cgcgcagttc aaagtggcga
     6361 aataattgtc gtgcatcggc acgaccttgc cgagccactt tttcaccagc tcgggatatt
     6421 cgcggatcgc ttccgaaatc gacaggaaga tcacgccggc gcgcttcaac tcctcgcgga
     6481 aggtggtcgc gacgctgacg ctgtcgaaca ccgcgtcgac cgcgaccttg cgcgcgccct
     6541 cgacccccgc gagcaccttc tgctcttcga tcgggatgcc gagcttcttg taaacctcga
     6601 ggatttcggg atcgacctcg tcgaggctgg aaagcttcgg tttcgccttg ggcgccgcgt
     6661 aataataggc atcttggtaa tcgatcggcg ggacgttgag cttcgcccag tcgggcgtct
     6721 ccatcgtctg ccagtggcgg aacgccttca gccgccagtc gagcatccat tcgggctcgt
     6781 tcttcttcgc cgagatgaag cggaccgtat cctcggtcag ccccttgggc gcgaagtcgg
     6841 tctcgatggt cgaggaccag ccatgctcat aatcggcgac cttcgcggcg gcgtcgtgcg
     6901 cggcctggtc cttgacgggg agttcggtgt tatcggtcat atcttttaac tcgtcgcccc
     6961 tgcgaaggca ggggccgcta tcgttttact cggcgccgct ggcatagact ctccagcggc
     7021 cgtgtcggga ggcatgtgac tcccggcacc cttcgcgggg gcgacgctca aactcgccag
     7081 gctgatcccg gccagcgcgc cgcgcaccgc gccgttgacg acgccccagt gcggctgcac
     7141 cttgcagctg ccctcgagtg aacagtcgtg gcggccctcg gcgacgcagg cggtgagggc
     7201 gatcggccct tcgaccgcct cgatgatatc ggctacattg atcgccgccg cgggccgcgc
     7261 gagcc
//
LOCUS       DATMSU010000445         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_93066, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000445 DATMSU010000000
VERSION     DATMSU010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93066"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VN034_11955"
     CDS             complement(<1..2022)
                     /locus_tag="VN034_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HWV61365.1"
                     /translation="MRRQAMVIATALLLAGTAMAATQDAGEAAAVHPELWPAAKSPAA
                     ITDAATEARIDALMKKMTIEQKVGQLIQGDISTITPKDLETYPLGSILAGGNSGPNGN
                     ERSSAADWAKLVGEFRAVSMKPQANGVAIPIIFGVDAVHGHNNIPGATLFPHNIGLGA
                     AHDPELIQRIGAVTAAEIAGSGIEWTFAPTLAVPQDLRWGRSYEGYAADPKLVADYAT
                     AMVLGLQGKLVAGKTIDASHVAATAKHFLADGGTFEGKDQGDAKIDEKELIAKHAMGY
                     PAAIDAGALTVMASFSSWQGIKHHGNKGLLTDALKNKMGFEGFVVGDWNGHGQVAGCS
                     VTDCAQSINAGLDMFMAPDSWKGLYETTLNQAKDGTIPAARLDDAVRRILRVKYKLGL
                     FPEGHVDRSVVKAVGTPEHLAVAREAVAKSLVLLKNNGNVLPIKAGARVLVTGPGADN
                     MAMQSGGWTISWQGTDVVRSDFPNGQTIWEALDKSVRDAGGVATLSEGGVFKEKPDVA
                     IVVFGEAPYAEFQGDVATLDYQPTEASDLATLKKLKAAGVPVVALFLSGRPMFTNPEI
                     NAADAFVAGWLPGSQGAGVADVLVAGKDGKTVRGFTGTLPFAWPSDARSPVEKPQFAV
                     GYGLKYGNATTVPQLSEELGVDISAALNVENFFSGGRARAPWTLSITDAG"
     gene            complement(2111..>2668)
                     /locus_tag="VN034_11960"
     CDS             complement(2111..>2668)
                     /locus_tag="VN034_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61366.1"
                     /translation="PSGPAVRMWLARLLVQIAEAALFAYLYFWFRSIDPDMHDNEKAR
                     IFGVALTLAIPIALASGRWADRNDRPFLPLVASAAVSAAGMIGMAFATELDMAKASYL
                     VFGVATTVFLSLHASQTLRILPRSDRRGRDLGIFNLTNTMPSLIMPWLTISLVPGFGF
                     DALFLLLAGLTAIAVLLLATMPRPR"
BASE COUNT          453 a          902 c          837 g          476 t
ORIGIN      
        1 gcccgcatcg gtgatcgaca gcgtccacgg cgcccgcgcc cggccgcccg agaagaaatt
       61 ctcgacgttc agcgccgccg aaatatcgac gcccaattct tccgaaagct gcggcacggt
      121 tgtcgcgttg ccatatttca gcccgtagcc caccgcgaac tgcggctttt cgaccggcga
      181 gcgcgcatcg gagggccagg cgaagggcaa ggtaccggtg aaaccgcgca ccgtcttgcc
      241 atccttgccc gcgaccagca catcggcgac gccggcgccc tgcgagcccg gcagccagcc
      301 cgcgacgaag gcgtcggcgg cgttgatttc ggggttggtg aacatcggcc gccccgagag
      361 gaaaagcgcg acgacaggaa cccctgccgc cttcagcttc ttgagcgtcg cgagatcgct
      421 cgcttcggtc ggctgataat cgagcgtcgc gacgtcgccc tggaattcgg catagggcgc
      481 ctcgccgaac acgacgatcg cgacatcggg cttttccttg aagacgccgc cctcggacaa
      541 ggtcgcgacg ccacccgcat cgcgcaccga cttgtcgagc gcctcccaga tcgtctgccc
      601 gttcggaaag tccgaccgca cgacgtcggt gccctgccaa ctgatcgtcc agccgcccga
      661 ctgcatcgcc atattgtcgg cgccgggccc ggtgacgagc acgcgcgcac ctgctttgat
      721 cggcaggaca ttgccgttat tcttgagcaa taccagcgac ttggcgaccg cttcgcgcgc
      781 caccgccaga tgctcgggcg tgccgaccgc cttcacgacg ctgcggtcga catggccctc
      841 cggaaacagg ccgagcttgt atttgacgcg caggatgcgg cgcaccgcat catcgaggcg
      901 cgcggcaggg atcgtgccgt ccttcgcttg attcagcgtc gtttcgtaaa ggcccttcca
      961 gctgtcgggc gccatgaaca tgtcgagccc ggcgttgatc gactgcgcgc agtcggtgac
     1021 gctgcatccc gcgacctgcc catggccgtt ccaatcgccg acgacaaagc cttcgaagcc
     1081 cattttgttt ttgagcgcgt cggtcagcag ccccttgttg ccgtgatgct tgatcccctg
     1141 ccagctcgaa aagctcgcca tcacggtcag cgcgccggcg tcgatcgcgg cgggatagcc
     1201 catcgcatgc ttcgcgatca gctccttctc gtcgatcttc gcgtcgccct gatccttgcc
     1261 ctcgaacgtt ccgccgtcgg cgaggaaatg cttggcggtc gccgcgacat ggcttgcatc
     1321 gatggtcttg cccgcaacca gcttcccctg cagcccgagc accatcgccg tcgcataatc
     1381 ggcgacgagc ttgggatcgg cggcatagcc ttcatagctg cggccccagc gcagatcctg
     1441 cggcaccgcg agcgtcggcg cgaaggtcca ttcgatgccg ctgcccgcga tttcggccgc
     1501 ggtgaccgcg ccgatacgct gaatcagctc gggatcgtgc gccgcgccga gcccgatatt
     1561 gtgcgggaac agcgtcgcgc cggggatatt attatgccca tgcaccgcat cgacgccaaa
     1621 gatgatcggg atcgcgacgc cattcgcctg cggcttcatc gagacggcgc gaaattcgcc
     1681 gacgagcttc gcccagtcgg ccgcgctcga tcgttcattg ccgttcggcc cgctgttgcc
     1741 gcccgccagg atcgatccga gcggataggt ttcgaggtcc ttgggcgtga tcgtgctgat
     1801 gtcgccctgg atcagctggc cgaccttctg ctcgatcgtc atcttcttca tcagcgcgtc
     1861 gatgcgcgcc tcggtcgcgg cgtcggtgat cgccgccggg ctcttcgccg cgggccagag
     1921 ttcgggatgg acggccgcgg cctcgcctgc gtcctgcgtt gctgccattg cggttcccgc
     1981 cagaagcagc gcagtcgcga tcaccattgc ctgtcggcgc atcgtctctc ccatcccatt
     2041 atgtgaacgt tatcaaaatt tgatgtcgca taggggccga atgaaatcaa gcactctatt
     2101 gggggcgggt tcagcgcggg cgcggcatcg tcgcgagcag gagcaccgcg atcgcggtca
     2161 gccccgcaag caacaggaaa agcgcgtcga atccgaagcc ggggaccagc gaaatggtca
     2221 gccagggcat gatcagcgac ggcatcgtat tggtcaggtt gaagatgccg aggtcgcgtc
     2281 cgcgccggtc cgaacgcggc aatatgcgca atgtctggct ggcatggagc gagaggaaca
     2341 ctgtcgtcgc gacgccgaac acgaggtagc tcgctttcgc catatcgagc tcggtcgcaa
     2401 aggccatgcc gatcatgcct gccgccgaca ccgccgcgct ggcgacgagc ggcaggaagg
     2461 gccggtcgtt gcggtcggcc cagcggccgc tcgccagcgc gatcggtatc gcaagcgtca
     2521 gcgcgacccc gaagatacgc gccttttcgt tatcgtgcat atcggggtcg atcgaccgga
     2581 accagaaata gagataggcg aagagcgccg cttcggcgat ctggaccagc aggcgcgcga
     2641 gccacatccg taccgcgggg ccgctcgg
//
LOCUS       DATMSU010000446         9932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_178152,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000446 DATMSU010000000
VERSION     DATMSU010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9932
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178152"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1258
                     /locus_tag="VN034_11965"
     CDS             119..1258
                     /locus_tag="VN034_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61367.1"
                     /translation="MQGFALYPFDPPGDIAALRADLRAWLAVNQPRGDVVSRANCWAS
                     FDPEFSRALGAAGYIGMTLPAQYGGGDRHPLERYVVIEELLAAGAPVGAHWIADRQTG
                     PLILRYGSEEQRQRYLPGIAAGELYACIGLSEPGAGSDLAAVRTTARETAEGWRINGQ
                     KIWTTGAHFSHIMLALVRTDEGSERNAGLSQLLIDLDTPGITIRPIIDMAGHHDFNEV
                     FFDDALVPHGALVGERGQGWKQVTAELGLERSGPERYLSSHALLVALIDAAGAEPDPA
                     IAALIGELTAEMWTLRQLSMSTAAKLAAGEDPMVEASVVKELGNAFEQDMPRRVQAIV
                     TSGWDDEDDLAKLLRALLIASPSFSLRGGTREVIRGIIARGLGLR"
     gene            1273..1956
                     /locus_tag="VN034_11970"
     CDS             1273..1956
                     /locus_tag="VN034_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61368.1"
                     /translation="MLCDTARAVFAEAATAGIAPVEEAGFGLLLVPEEDGGFGGDWGD
                     ANAVLQIAGVVVPDLPVAELIAGEAYQPAATVSLMAGAMGQALALSIEHVNTRQQFGR
                     PLGKFQAVQQSLAVMACEVRAVEAAAAALATRLDAMSLDPSAADFEIAAAKLRANRAV
                     GVVTAIAHQVHGAIGFTEEYDLHRVTVPLMRWRGAHGNDAYWAARLGRQVATLGGRGL
                     WEAMTARGA"
     gene            complement(1961..3091)
                     /locus_tag="VN034_11975"
     CDS             complement(1961..3091)
                     /locus_tag="VN034_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWV61369.1"
                     /translation="MSGPLKGIRIIEFAGIGPGPFCGMMLADHGAEVIRIDRPGGFMD
                     PRDPLSRNRTSIALDMKNPAAVQIARELCKSADGIIEGYRPGVMERLGLGPEVLLADN
                     PKLVYGRMTGWGQFGPYAQAAGHDINYISLSGVLHGIGRAGEKPVPPVNYVGDFGGGG
                     MMLAFGMSSALVHAARTGEGQVIDCAMTDGSALLAGMTWGFHAAGRLKDEAGVNMLDG
                     AAHFYDSYVCSDGKYISIGSIEPQFYALLREKTGLTDDADFDAQLDPSKWGPLKEKLT
                     ALFKTKTRDEWCAIMEMTDVCFAPILSLSEAPQHPHNVERETFLTVGGAVQPAPAPRY
                     SATVNDTPRPAPAVGADGDAVLAGLGYDADRIAALREGGALR"
     gene            complement(3108..4358)
                     /locus_tag="VN034_11980"
     CDS             complement(3108..4358)
                     /locus_tag="VN034_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWV61370.1"
                     /translation="MAEAYIIDAVRTPRGIGKPGKGALSHLHPQHLAATVLAAIRDRN
                     NLDTATVDDIVWSTSSQNGKQGGDLGRMAALSAGYDTKASGTTLDRFCGGGISSVNFA
                     AASVMSGMEDCVIAGGTEMMSYTTAHAAEQANAGLPPRLMGSGHEALDELHPQSHQGI
                     CGDAIATIEGISREALDALALVSQQRADRAIKEGRFNKSVVPVLNTDGSVALDHEEFP
                     RPETTAEGLASLKPAFTGLADFDLGGGFTFRKQVNRRYPDVEIQHFHHAGNSSGVVDG
                     AAALLLTSKDYADKHGLKPRARVVAYANIGDDPTLMLNAPVPAAKKVLEKAGLTKDDI
                     DVWEINEAFSVVAEKFIRDLALDREKVNINGGAMALGHPIGATGSILIGTALDELERS
                     GGRYGLVTMCAAGGMAPAIIIERI"
     gene            complement(4384..5166)
                     /locus_tag="VN034_11985"
     CDS             complement(4384..5166)
                     /locus_tag="VN034_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61371.1"
                     /translation="MKIDSSIAAVVTGGASGLGRATAEALAASGVKVAIFDINDALGE
                     EVAASIGGLFVHVDITDEQSVLDGYAKARGAHGQERVCVHCAMTSRRGKTLAYDKETG
                     QFRRTPTEDYAYGVAGILTASYRIGSIAAEGMATLPEMEDGERGAIIFTASVAAQDAQ
                     IGQVIYGSAKAGVNGLVLPMARDLMDLGIRVNSIMPGVFGTPLLGNMNPKVKESLEAS
                     VPFPKRLGKAEEYASLAMEMVRNNYFNGQAVRLDGAIRMAPR"
     gene            complement(5178..6383)
                     /locus_tag="VN034_11990"
     CDS             complement(5178..6383)
                     /locus_tag="VN034_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61372.1"
                     /translation="MTNLETYRAKAAAWCESMVPAFGKAARKGLSVEEDLALGRRYQK
                     AKFDAGYAGINWPTDLGGQGLGHIEKITFEGEEMKHGFPNVYFGISLGMPVPVLMQFG
                     SDREFVKERVLKALKGEEIWCQLFSEPSGGTDLAGLRTRAETDGNGWKINGQKVWTSW
                     AQYSDYGVIVVRTDPTVAKHKGLTYFWVDMKAPGVTVRPIKLAGGDSHVNEVFFDDVK
                     ISDDHRMSPVGGGFAVALATLMIERYVATDSAGFGPHLDLFVELAKEVKLNGRPAIED
                     GRIRQQIARNHAMRAGLDSINRRARLMMQAGMTPGPEGSLNKLVAVRSRQKLSELALD
                     LQGTGAFLFDEHASSKDDWGSSWINAPTGRIAGGSDETLLNTIAERILGLPQDHRPDK
                     GIPFNQIPA"
     gene            complement(6380..7474)
                     /locus_tag="VN034_11995"
     CDS             complement(6380..7474)
                     /locus_tag="VN034_11995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61373.1"
                     /translation="MSILYDEGQQAIATESRRAIEARVSKDDLLPLLQASGKYHEGFW
                     ATAKEQGWTALALPEAYGGLDLGLVELGLIAHQAGRSLSGAPFLTSSFGAAKAIELYG
                     NDEQKTRWLPGLASGETIGAIAFAQGPDPLPAAPSIKLNAGRVEGTAVGVSGGLFADI
                     AIALVDEGGSPALAIVDLTGIERKAVDSFDNSRCIADLLFAGAQAETLVTGDAARAAA
                     LNILALQAVVTAHEQTGGAEALMEIARDYALTRKAFGQPIGAFQSIKHRIAELYGLVE
                     LSRANAIHAAAREGQDDFITAAAAARLSATEAYDTAARDCVQIHGGIGVTWEIGLHLH
                     MRRARTLALEQGSSFFWEDILVDRLAGAGA"
     gene            complement(7579..8760)
                     /locus_tag="VN034_12000"
     CDS             complement(7579..8760)
                     /locus_tag="VN034_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61374.1"
                     /translation="MAAHEGISERARAIAEKVESFVRETVVPFETDPRRDHHGAPTDE
                     LVMEMREHARTAGVLTPHILSDGSHLNQRETAVVLIKSGLSPLGPLACNTMAPDEGNM
                     YLLGKEGSPELKEQFLKPMVEGRVRSAFFMTEPADEGGAGSDPSMMKTTCHPDGNHWV
                     INGRKTFITGAQGARVGIVMAKADQGACMFLIDLPDPAIRIDHVPNTIDSSMPGGHAT
                     LTIDNLRVPADQMLGEAGEGFRYAQVRLSPARLSHCMRWLGACIRAHEIATDYANRRE
                     AFGKPLIDHEGVGFMLAENMIDLKQAELMIDWCAGVLDTGSLGTVESSMAKVAVSEAL
                     MRIADRCVQVMGGTGVTDKTIVEQVFREVRAFRIYDGPTEVHKWSLAKKIRRDWKAGL
                     A"
     gene            8881..9861
                     /locus_tag="VN034_12005"
     CDS             8881..9861
                     /locus_tag="VN034_12005"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HWV61375.1"
                     /translation="MAFKTRITEMLGIEHPIVQGGMQSVGYAELASAVSNAGGLGILT
                     ALTQPTPEALRDEIERCRAMTDKPFGVNLTVFPTINAPDYKAYAQAIIDGGVKIVETA
                     GTQAVREIWEMLKPHGVTILHKCTAVRHALSAERAGCDIISIDGFECAGHPGEDDIPG
                     LILIPAAADKVKIPMLASGGFGDGRGLVAALSLGAEGINMGTRFCATKEAPIHDNVKQ
                     AYVDNDERGSFLIFRSLKNTARVGKSAVSEEVVRRLAVPDATFADVAELVNGKAGREL
                     LETGDLSKGVFWAGMVQGLIHDIPTCQELIDRIMSEADAIVDQRLASLRA"
BASE COUNT         1639 a         3224 c         3253 g         1816 t
ORIGIN      
        1 tctcgggctt gatcatcgtc aggatcgcga tcttgccccg ccgctcgaca ctcagaaatt
       61 cgcccatctt caattatcct ttgctgacta accaaagcta ttgcatggct ggtgcgacat
      121 gcaaggcttt gcgctctatc cgttcgatcc acccggcgac attgccgcac tgcgcgccga
      181 tttgcgggca tggcttgcgg tgaaccagcc gcggggtgac gtggtttccc gggcgaactg
      241 ctgggcgagt tttgaccccg agttcagccg tgcgctcggc gcggcggggt acatcggcat
      301 gaccttgccg gcgcagtacg gcggcggtga ccggcatccg ctcgagcgct atgtcgtgat
      361 cgaggaattg ctggcggcgg gcgccccggt cggagcgcac tggatcgctg atcgccagac
      421 cgggccgctg atcctgcgct atggcagcga ggaacagcgg cagcgctacc tgccgggaat
      481 tgcggcgggc gagctttatg cgtgcatcgg cctgtccgaa cccggcgcgg gctcggacct
      541 ggctgccgta cgaacgactg cgcgcgaaac tgccgagggc tggcgcatca acggccagaa
      601 aatctggacc accggcgcgc atttctcgca catcatgctc gcgctcgtcc gcaccgacga
      661 gggcagcgag cgcaacgccg ggctcagcca gctgctgatc gacctcgaca cccccggcat
      721 cacgatccgc ccgatcatcg acatggcggg ccatcacgat ttcaacgagg tttttttcga
      781 cgacgcgctg gtgccgcatg gcgcattggt cggcgagcgc gggcaggggt ggaaacaggt
      841 caccgccgaa ctcggcctcg agcgttcggg ccccgaacgc tatctgtcaa gccacgcact
      901 gctcgtcgcg ctgatcgatg cggcgggcgc cgaacctgat ccagcgattg ccgcgttgat
      961 cggcgaactc accgccgaga tgtggacgct gcgccaactc tcgatgtcga ccgcggcgaa
     1021 gctcgcggcg ggcgaagacc cgatggtcga agcgtcggtg gtcaaggaac tcggcaatgc
     1081 cttcgaacag gacatgccgc gccgcgtgca ggcgatcgtg acaagcggtt gggacgatga
     1141 agatgatctt gcgaagcttc ttcgcgcgct gcttattgcg tcgcccagct tctcgctgcg
     1201 cggcggaacg cgcgaggtga tccgcgggat catcgcacgc ggattgggcc tccggtgagc
     1261 gcggcgcacg acatgctgtg cgataccgcg cgcgcagtct tcgcggaggc cgcgacggcg
     1321 gggatcgctc cggtcgagga agcgggcttt ggcctgctgc ttgttcccga agaggatggc
     1381 ggtttcggcg gcgactgggg cgacgcgaat gcggtgctgc aaatcgccgg tgtggtcgtg
     1441 cccgatctcc cggttgccga actgatcgcc ggcgaagcgt atcagcccgc ggcgacggtg
     1501 agcctgatgg ccggtgcgat ggggcaggcg cttgcgctct cgatcgagca tgtgaacaca
     1561 cgccagcagt tcggccgccc gctcggcaag ttccaagcgg tccagcaatc gctcgcggtg
     1621 atggcgtgcg aagtgcgcgc ggtcgaagcc gccgctgcgg cgctggcgac acgcctcgat
     1681 gcgatgagcc tcgatccgtc agccgccgat ttcgaaatcg cggcagcgaa gctccgtgcc
     1741 aatcgcgccg tcggcgtggt caccgcgatt gcgcatcagg tgcacggtgc gatcggcttt
     1801 accgaggaat atgacctgca ccgcgtgacg gtgccgctga tgcgctggcg cggggcgcac
     1861 ggaaacgacg cctattgggc ggcgcggctg ggccgtcagg ttgccaccct tggcgggcgc
     1921 ggcctctggg aagcgatgac cgcgcgcggc gcttgaacaa tcagcgcagc gcgccacctt
     1981 cgcggagcgc ggcaatcctg tctgcatcat aacccagacc cgccagcacg gcgtcgccat
     2041 cagcgccgac tgccggtgcc ggccgcggcg tgtcgttgac cgttgccgaa tagcgcgggg
     2101 caggggcggg ctgaaccgcg ccgccgacgg tcaggaaggt ttcgcgctcg acattgtgcg
     2161 gatgctgcgg cgcttcgctc agcgacagga tcggcgcgaa gcagacatcg gtcatttcca
     2221 taattgcgca ccattcgtcg cgcgttttgg tcttgaacag ggcggtcagc ttttccttga
     2281 gtggccccca tttcgacggg tcgagttggg cgtcgaaatc ggcatcgtcg gtcagcccgg
     2341 tcttctcacg cagcagcgca tagaattgcg gctcgatcga cccgatcgag atatacttgc
     2401 catcggaaca gacatagctg tcatagaaat gcgccgcgcc gtcgagcatg ttgaccccgg
     2461 cttcgtcctt gagccgaccc gcggcgtgga agccccacgt cataccggca agcaacgccg
     2521 agccgtcggt catcgcgcag tcgatcacct ggccctcgcc ggtgcgcgcg gcatggacaa
     2581 gcgcgctcga catgccaaag gcgagcatca tgccgccgcc gccgaaatcg ccgacataat
     2641 tgaccggcgg gacgggcttt tcgcccgcgc gcccgatgcc gtggagcaca cccgacagcg
     2701 agatatagtt gatgtcgtgt cccgccgcct gggcataggg gccgaactgg ccccagccgg
     2761 tcatgcgccc gtaaacgagc ttcggattgt ccgcgagcaa cacctccgga ccgagcccga
     2821 gccgctccat cacgccgggg cgatacccct cgatgatccc gtcggcgctt ttgcagagtt
     2881 cgcgcgcgat ctgcaccgcc gcgggatttt tcatgtcgag cgcgatcgac gtgcgattgc
     2941 ggctcagcgg gtcgcgcggg tccatgaaac cgccggggcg atcgatacgg atcacctcgg
     3001 cgccatggtc ggcgagcatc atgccgcaaa aggggccggg accgatgccg gcaaattcga
     3061 tgatcctgat acccttcagc ggcccggaca tgatgctctc ctgttcctca gatacgttcg
     3121 atgatgatcg cgggtgccat gccgcccgcg gcgcacatgg tgacgaggcc atagcggccg
     3181 cccgagcgtt cgagctcgtc gagcgcggtg ccgatcagga tcgagccggt cgcgccgatc
     3241 ggatggccga gcgccatcgc gccgccgttg atgttcacct tttcgcggtc gagagcgagg
     3301 tcgcggatga acttctcggc gacgaccgag aaggcttcgt tgatttccca gacgtcgatg
     3361 tcgtccttgg tcagcccggc cttttcgagc accttctttg cggcgggaac gggcgcgttg
     3421 agcatcagcg tcggatcgtc gccgatattg gcataggcga cgacgcgcgc gcgcggcttc
     3481 aacccatgct tgtcggcata atccttcgag gtgaggagca gcgcagcggc accgtcgacg
     3541 acacccgaag agttgccggc gtgatggaaa tgctggattt cgacgtcggg atagcggcga
     3601 ttgacctgct tgcggaaggt gaagccgccg ccgagatcga aatcagcgag gccggtgaag
     3661 gcaggcttca acgatgcaag gccttcggca gtcgtttcgg gccgcgggaa ttcctcatgg
     3721 tcgagtgcga cgctgccgtc ggtattgagc accggcacga ctgacttgtt gaaacggcct
     3781 tccttgatcg cgcggtcggc gcgctgctgg ctgacgagcg cgagcgcgtc gagcgcctca
     3841 cggctgatgc cctcgatcgt cgcgatcgcg tcgccgcaaa tgccctggtg cgactgcggg
     3901 tgcagctcgt cgagcgcttc gtggcccgat cccatcaggc gcggcggcag accggcattt
     3961 gcctgttcgg cggcatgggc ggtcgtgtag ctcatcattt cggtgccgcc cgcgatgacg
     4021 cagtcttcca tgccgctcat cacgctcgcc gcggcaaagt tgaccgaact gatcccgccg
     4081 ccgcagaaac gatcgagcgt cgtgccgctc gccttggtat cgtagcccgc cgacagtgcc
     4141 gccatgcggc cgaggtcgcc gccctgcttg ccgttctggc tcgacgtcga ccagacgatg
     4201 tcgtcgaccg tcgcggtgtc gagattattg cggtcgcgga tcgcggcgag caccgttgcg
     4261 gcgagatgct gcgggtggag atgcgacagc gcgcccttgc cgggcttgcc gatcccgcgc
     4321 ggggtgcgga cggcgtcgat gatataggct tcggccatag gaaattcctt tcgtcgggag
     4381 agattaacgg ggggccatgc ggatcgcgcc gtcgaggcgg accgcctggc cgttgaaata
     4441 attgttgcgg accatttcca tcgcgaggct cgcatattcc tcggctttgc cgagccgttt
     4501 ggggaagggg accgatgctt cgaggctttc cttcaccttc gggttcatat tgccgagcag
     4561 cggcgttccg aacacgccgg gcatgatcga gttgacgcgg atgccaaggt ccatcaggtc
     4621 gcgcgccatt ggcaacacga ggccgttcac gcccgccttc gccgatccgt agatgacctg
     4681 cccgatctgc gcgtcctgcg ccgcgaccga cgcggtaaag atgatcgcgc cgcgctcgcc
     4741 gtcttccatt tccgggagtg tcgccatgcc ttcggccgcg atcgatccga tgcgatagct
     4801 cgcggtcaat atgcccgcga cgccataggc gtaatcctcg gtcggcgtgc ggcggaactg
     4861 gcccgtttcc ttgtcataag cgagcgtctt gccgcggcgc gacgtcatcg cgcagtggac
     4921 gcatacgcgt tcctgcccat gcgccccgcg cgccttggca tagccgtcca agaccgattg
     4981 ctcgtcggtg atgtcgacat ggacgaacag cccgccgatc gacgccgcga cttcttcgcc
     5041 gagcgcgtcg ttgatgtcga agatcgcgac cttcaccccc gacgcggcaa gcgcctcggc
     5101 ggttgcgcgg ccgaggcccg atgcgccgcc ggtgaccacc gctgcgatgc tggaatcgat
     5161 tttcatggcg aggctcctca ggccggaatc tggttgaagg ggatgccctt gtcggggcga
     5221 tggtcctgcg gcaggccgag gatgcgctcg gcgattgtgt tgagcagagt ctcgtccgaa
     5281 ccgcccgcga tgcggcccgt cggcgcgttg atccagctcg acccccagtc gtccttggac
     5341 gaggcgtgtt cgtcgaacag gaaggcgccc gttccttgca gatcgagcgc gagttcggac
     5401 agtttctggc gcgagcgcac cgcgacaagc ttgttgagcg aaccttcggg acccggcgtc
     5461 attcccgcct gcatcatcag ccgcgcgcgg cggttgatcg agtcgagtcc cgcgcgcatc
     5521 gcatggttgc gcgcgatctg ctggcggatg cggccatcct cgatcgcggg gcggccgttc
     5581 agcttcacct ctttcgcaag ctcgacgaac agatcgaggt gtgggccgaa ccccgcgctg
     5641 tcggtcgcga catagcgctc gatcatcagc gtcgcgagcg cgacggcgaa gccgccaccg
     5701 accggcgaca tgcgatggtc gtcgctgatc ttcacatcgt caaagaagac ttcgttgacg
     5761 tggctgtcgc cgcccgcgag cttgatcgga cgcaccgtca cgcccggcgc cttcatgtcg
     5821 acccagaagt aagtgaggcc cttgtgcttc gcgaccgtgg ggtcggtgcg cacgacgatg
     5881 acgccatagt cgctatattg cgcccagctc gtccacactt tctgcccgtt gatcttccag
     5941 ccgttgccgt cggtctcggc gcgcgtgcgc aggcccgcga ggtcggtgcc gcccgagggt
     6001 tcggaaaaga gctggcacca gatttcctcg cccttgagcg ccttgagcac gcgttccttc
     6061 acgaactcgc ggtcgctacc gaactgcatc agcacgggga cgggcatgcc gagcgagatg
     6121 ccgaaataga cgttggggaa gccgtgcttc atctcctcgc cctcgaaggt gattttctca
     6181 atatgaccga gcccctggcc gccgagatct gtcggccagt tgatccccgc atagcccgcg
     6241 tcgaacttcg ccttttgata gcggcggccg agcgcgagat cctcctcgac gctcagcccc
     6301 ttgcgcgccg ccttgccgaa cgcgggcacc atcgattcgc accaggcggc cgccttggca
     6361 cggtaggttt cgaggttggt catgcgcctg ctcctgccaa ccggtcgacg agtatatctt
     6421 cccagaagaa gctgctgccc tgctcgagcg cgagggtgcg cgcgcggcgc atgtgaaggt
     6481 gcaatccgat ctcccacgtc acgccgatgc cgccgtgaat ctggacgcaa tcgcgcgccg
     6541 cggtgtcgta ggcttcggtc gccgacaggc gcgctgcggc ggcggcggtg atgaagtcat
     6601 cctgtccctc gcgtgccgcg gcgtggatcg cgttggcgcg cgacagttcg acgaggccgt
     6661 aaagctcggc gatgcgatgt ttgatcgact gaaacgcgcc gatcggctgg ccgaacgcct
     6721 tgcgcgtcaa ggcatagtcg cgcgcgatct ccatcagcgc ctcggcgccg ccggtctgct
     6781 cgtgcgcggt cacgaccgcc tgcagcgcga ggatgttgag cgccgccgcg cgtgcggcgt
     6841 ccccggtcac cagcgtttcc gcctgcgcgc cggcgaacag caggtcggcg atacagcggc
     6901 tgttgtcgaa gctgtcgacc gccttgcgct cgatcccggt gaggtcgacg atcgccagcg
     6961 ccggcgaacc gccttcatcg acaagtgcga tggcgatatc cgcgaagagt ccgcccgaaa
     7021 cgccgaccgc ggtcccttcg acccgaccgg cattcaactt gatcgaaggc gctgcgggca
     7081 ggggatcggg cccttgcgcg aaagcgatgg cgccgatcgt ctcaccactc gcaagccccg
     7141 gaagccaacg cgtcttttgc tcgtcgttgc cgtaaagttc gatcgccttc gccgcgccaa
     7201 agctcgacgt caggaacggc gcgccgctca gcgaccggcc ggcctgatgc gcgatcagcc
     7261 cgagttcgac cagcccgaga tcaagcccgc cataggcttc gggaagcgcg agcgcggtcc
     7321 agccctgttc cttcgccgtc gcccagaagc cctcatgata tttgccgcta gcctggagca
     7381 gcggaagcag atcatccttg ctgacccgcg cctcgatcgc gcgccgcgac tcggtcgcga
     7441 tcgcctgctg tccttcgtcg tacaagatcg acatcggccc gattcctcac cctctagttt
     7501 ccggtcatca tccggacgtt aacgtaaacg tcaagcgttt tgacgctacg tctcgatgac
     7561 cgtcaactgg catgcccgtc aggcgagccc ggctttccag tcgcggcgga tcttcttggc
     7621 gaggctccat ttgtggacct cggtcgggcc gtcgtagatg cggaacgcgc gaacctcgcg
     7681 gaaaacctgt tcgacgatcg tcttgtcggt gactccggtg ccgcccatca cctggacgca
     7741 gcggtcggcg atgcgcatca gcgcctccga caccgcaacc ttggccattg agctttcgac
     7801 ggtgccgagc gatccggtgt cgagcacgcc cgcgcaccag tcgatcatca gctcggcctg
     7861 tttcaggtcg atcatatttt cggcgagcat gaagccgaca ccttcatggt cgatcagcgg
     7921 cttgccgaac gcttcgcggc ggttggcata gtcggtggcg atctcgtgcg cgcggatgca
     7981 tgcgccgagc cagcgcatgc agtgcgacag gcgggcaggg gagaggcgga cctgtgcgta
     8041 acgaaagcct tcgccggcct cgccgagcat ctgatcggcg ggaacgcgca gattgtcgat
     8101 cgtcagcgtc gcgtgaccgc cgggcatcga gctgtcgatc gtattgggaa catggtcgat
     8161 gcggatcgcc ggatcgggca ggtcgatcag gaacatgcac gcgccttggt cggccttcgc
     8221 catcacgatg ccgacgcgcg cgccctgtgc gccggtgatg aaggtcttgc ggccgttgat
     8281 gacccagtgg ttgccatcgg gatggcatgt cgtcttcatc atcgatggat cggaccccgc
     8341 gccgccctcg tcggcgggtt cggtcatgaa gaaggccgaa cggacgcgcc cctcgaccat
     8401 cggtttcagg aattgttcct tgagttcggg gctgccctcc ttgccgagca gatacatatt
     8461 gccttcgtcg ggggccatcg tgttgcacgc gagcggcccg agcggcgaca ggccgctctt
     8521 gatgagaaca acggccgtct cgcgctgatt gaggtgactg ccgtcggaca ggatatgcgg
     8581 cgtcagcacg cccgcagtgc gcgcatgctc gcgcatctcc atgacgagtt cgtcggtcgg
     8641 cgcgccgtga tggtcgcggc gcggatcggt ttcaaagggg acgacagttt cgcgaacgaa
     8701 gctttcgacc ttttcggcga tagcccgcgc ccgttccgat atgccttcgt gcgccgccat
     8761 tccgattccc ttcgctatct aaccttcttt ctagtcaacc cgacgttgac gtaagcgtca
     8821 agttatttca tggaccggaa cgaagatgcc gtaagggcag agccgcacag gaggatcgat
     8881 atggctttca agacacgcat caccgaaatg ctgggtatcg aacatccgat cgttcagggc
     8941 gggatgcaga gcgtcggtta cgccgaactc gcgagcgcgg tgtcgaacgc cggcggcctc
     9001 ggtatattga ctgcgctgac ccagccgacg cccgaggcgc ttcgcgacga gatcgagcgc
     9061 tgccgtgcga tgaccgacaa gccgttcggc gtgaacctga cagtgttccc gacgatcaac
     9121 gcccccgatt acaaggccta tgcgcaggcg attatcgacg gcggggtcaa gatcgtcgaa
     9181 accgcgggca cgcaggcggt gcgcgagatt tgggagatgc tcaagcccca tggcgtcacc
     9241 atcctccaca aatgcaccgc cgtgcgccac gcgctgtcgg ccgagcgcgc gggctgcgac
     9301 atcatctcga tcgacggctt cgaatgcgcg ggccaccccg gcgaggacga tattccgggc
     9361 ctgatcctga tccccgccgc cgccgacaag gtgaagatcc cgatgctcgc gtcgggcggc
     9421 ttcggcgacg gccgcgggct ggtcgcggcg ctgtcgctcg gcgccgaggg catcaacatg
     9481 ggcacgcgct tctgcgcgac caaggaagcc ccgatccacg acaatgtgaa acaggcctat
     9541 gtcgacaatg acgagcgcgg cagcttcctg atcttccgca gcctcaagaa caccgcgcgc
     9601 gtcggcaaga gcgcggtgag cgaagaggtg gtgcgccgcc tcgcggtccc cgacgcgacc
     9661 ttcgccgacg tcgccgaatt ggtgaacggc aaggcgggcc gcgaattgct cgaaaccggc
     9721 gacctgtcga agggcgtttt ctgggccggc atggtgcaag gcctcatcca cgatatcccg
     9781 acctgccagg aactgatcga ccggatcatg tcggaagccg acgcaatcgt cgatcagcgc
     9841 ttggcctcgc tgcgcgcgta attcgcccgg cgcgctggcg cgccgccacg gctttcgata
     9901 tgatccgatt cttcgcaatt tgatcatatc ga
//
LOCUS       DATMSU010000447         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_8069, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000447 DATMSU010000000
VERSION     DATMSU010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8069"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..791)
                     /locus_tag="VN034_12010"
     CDS             complement(144..791)
                     /locus_tag="VN034_12010"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV61376.1"
                     /translation="MTIDPRQLQNILAEHLPVFAVKAPHYQVEMLDSLLEVWQGRPQH
                     LLDVGGGTGVIAQAIADLFPVASVEAIDLIDRFCPTLKVPTRQYDGRSIPCADGSYDA
                     ATLNNVIHHVPPGARADLMREIRRAVAGPVYIKDHLTTGFIDDMRLTALDAWGNIPFG
                     GMVKANYLSQAEWDELARASGYRIGAVAGSRAYRTGFAGLAFPNRLETTMRFDPA"
     gene            complement(808..1191)
                     /locus_tag="VN034_12015"
     CDS             complement(808..1191)
                     /locus_tag="VN034_12015"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWV61377.1"
                     /translation="MIARPALAYGAVSATCLVLHNAVMIGGDWLGWPYFGSILVSFSL
                     SAVTGYVLHSLVTFGKPISMRHFARYAIAMAANIPLAFVAIWIWHDIAGLAMLWASPI
                     ATICMIAVNFLLSRWAILSQSTRMI"
     gene            complement(1188..1874)
                     /locus_tag="VN034_12020"
     CDS             complement(1188..1874)
                     /locus_tag="VN034_12020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWV61378.1"
                     /translation="MRESRDNPRIWDTDWLVLRPLARLVREKVGAHSPPSARLIDLGC
                     GDMPYKAALEQQGIRYIGADIDDGGDVRIAPDGRVPLEAGCFDAVLSVQVLEHVRDLD
                     AYFGEIRRLLGDDGILYLSTHGSWLYHPHPEDHRRWTSTGLVAELDAHGFTVEENVAL
                     VGPLATTTLIRMTGFAFFLRKIPLLGPAVANALTVIMNLRASIEDKLTPAAIRQNDAC
                     VYFVRARCKA"
     gene            complement(1871..2803)
                     /locus_tag="VN034_12025"
     CDS             complement(1871..2803)
                     /locus_tag="VN034_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWV61379.1"
                     /translation="MIGTVIERFKAMLPDADIYVYDNNSSDNSAEIAHRAGAIVRSEK
                     RQGKGFVVRRMFADIDADIYLMCDADETYEAEAAPDLIRQLVSGGLDMVVGTRTDVSG
                     TAYRPAHRFGNWMLTSLVRTIFGGGFRDMLSGYRVFSRRFVKSFPPMSQGFEIETELT
                     VHALQLEMPAGEAATRFKDRVEGSESKLQTVSDGFRILWTIAALLKQERPFALFGTAA
                     LALFCLSLIFGYPVVTEYIRSGTVPRLPTAILASGTMVLACLSLFSGLILDTVTRGRK
                     EAKLLRYLDVPGVHARLTTRNDGLTGLAPSGKIQ"
     gene            2702..2968
                     /locus_tag="VN034_12030"
     CDS             2702..2968
                     /locus_tag="VN034_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61380.1"
                     /translation="MGDLGGVVAAVIVVNIDVGVGEHRLETLDHRADHEAFVVAGQKN
                     RDGQGGVFMREQARSFELSGAHIHVSGNPHEIEPISLGKLGETS"
BASE COUNT          652 a          948 c         1020 g          546 t
ORIGIN      
        1 gtcgctcgcg gcccatgccg cgacatgccc ggcggccgcg aattcgcggt tcaggttggc
       61 cgcgacgcgc tccagcccgc cgccgtgcag atcgtagaaa ttgctgaccg acagaatgtg
      121 catcggatac aggccaaaag ccgtcaggcg ggatcgaagc gcatcgtcgt ttcgagccgg
      181 ttggggaagg ccagtcccgc gaatccggta cggtaggccc gtgagccggc aacagcaccg
      241 atgcggtacc cggacgcgcg cgccagttcg tcccattcgg cttgggagag atagttcgct
      301 ttcaccatgc cgccaaaggg aatgttgccc caagcgtcaa gcgccgtcag gcgcatatcg
      361 tcgatgaagc ccgtggtcag atgatccttg atatacaccg ggccggcaac ggcgcgccgg
      421 atttcgcgca tcagatcggc gcgcgcacca ggcgggacat gatggatgac attgttgagc
      481 gtagcggcat catagcttcc gtcggcacaa ggaatgctgc ggccgtcata ttggcgtgtc
      541 ggaaccttca gcgtcggaca aaagcgatcg atcaggtcga tcgcctcgac cgatgcgacc
      601 ggaaaaagat cggcgatggc ctgcgcgatc acgcctgttc cgccgccgac atcgagcaga
      661 tgctgcggac gaccctgcca gacttccagc agactatcga gcatctcgac ctgataatgt
      721 ggcgccttca cggcgaacac aggaagatgt tcggcgagga tgttttggag ttgacgcgga
      781 tctatggtca taggtgcagc ggccatatca gatcatccgt gtggattggg aaaggatcgc
      841 ccagcggctc agcaagaaat tgacggcgat catgcagata gtggcgatcg gggaggccca
      901 cagcatcgcg agaccggcaa tatcgtgcca tatccagatc gcgacgaacg cgagaggaat
      961 atttgccgcc atcgcaatcg cgtagcgcgc gaagtgacgc atcgagatcg gtttgccgaa
     1021 cgtaacgagg ctgtgcagca catagcccgt cacggccgac aggctgaagg aaacgaggat
     1081 cgagccgaaa tagggccagc cgagccagtc accgccgatc atcacggcat tgtggagcac
     1141 gaggcaggtc gcggaaacgg caccataggc aagggccggc cgcgcgatca cgctttgcac
     1201 cgcgcgcgga caaaatagac gcaggcatca ttctggcgga tcgcggcggg agtcagcttg
     1261 tcctcgatcg acgcacgcag gttcattatc acggtaagcg cattggcgac ggcagggccg
     1321 agcagcggaa tcttgcgcag gaaaaaggca aagccggtca tgcgaatgag cgtcgtcgtt
     1381 gcgagcgggc cgacaagggc gacgttttcc tcgactgtga aaccgtgcgc gtcgagttct
     1441 gcgacgaggc ccgtgctcgt ccaccggcga tggtcctccg gatgcggatg atagagccag
     1501 ctgccgtgag tcgagagata gagaatgccg tcgtcgccga gcaggcggcg aatttccccg
     1561 aaataggcgt cgaggtcgcg aacatgctcg agaacctgca ccgagaggac ggcatcgaag
     1621 catcccgctt cgagcggcac gcggccgtcg ggcgcgatcc gcacatcccc gccgtcgtcg
     1681 atgtcggcac cgatatagcg gattccttgc tgttcgagcg ccgccttata aggcatgtcg
     1741 ccgcagccca gatcgatcag ccgggcactg ggcggactat gcgcgccgac tttttcgcga
     1801 acgagccgcg ccagcgggcg caggaccagc cagtctgtgt cccatatgcg ggggttgtcg
     1861 cggctttccc tcactggatt tttccgctcg gcgccagacc cgtcagacca tcgttccgcg
     1921 tcgtgagacg cgcatgcacg cccgggacat caaggtagcg cagcaacttg gcctctttcc
     1981 ggccgcgcgt tacggtatcg agaatgagcc cgctgaaaag gctgaggcag gcgagaacca
     2041 tcgttccgct cgccaggatc gcggtgggca agcgcggcac ggtcccgctg cgtatatatt
     2101 cggtgacaac cggatagcca aagatcagcg ataggcagaa cagcgcgagt gccgccgtgc
     2161 cgaacagggc gaagggtcgc tcctgcttga gcagcgcggc aatcgtccac aggatgcgaa
     2221 agccgtccga cacggtctgc agcttgcttt cggacccttc gacgcggtcc ttgaaacgtg
     2281 tcgcagcttc gccggcaggc atttcgagct gcagcgcatg gaccgtcaat tcggtttcaa
     2341 tctcgaagcc ctgcgacatc ggcgggaagg atttgacgaa gcgacgggaa aaaacacgat
     2401 atccgctcag catatcgcga aacccgccgc cgaatatcgt ccgcaccagc gaggtcaaca
     2461 tccagttgcc aaagcggtgg gcgggccgat aggcagtgcc gctgacgtcg gtcctcgtgc
     2521 cgaccaccat gtcgaggccg ccgctgacga gctgcctgat caggtcgggc gctgcctcgg
     2581 cctcataggt ttcgtcggca tcgcacatga gatagatatc ggcatcgatg tcggcgaaca
     2641 tgcggcggac gacgaaaccc ttgccttgcc gcttctcgga gcgaactatg gccccggcgc
     2701 gatgggcgat ctcggcggag ttgtcgctgc tgttattgtc gtaaacatag atgtcggcgt
     2761 cggggagcat cgccttgaaa cgctcgatca ccgtgccgat cacgaggcct tcgttgtagc
     2821 agggcagaag aaccgcgatg gtcagggcgg cgtcttcatg cgggaacagg ctcgcagctt
     2881 cgaactgtcc ggcgcgcata tacatgtttc aggaaacccc cacgaaatcg aaccaatttc
     2941 tttagggaag ctaggcgaaa ctagttaaca actgcttagg agtcgggatc gccggccgcg
     3001 ttgcggcggg ccgggctgtg gctcaatccc ccagatcgac cccatgtttt acggcccaat
     3061 cccgatactg gccgcgcggg tcggccgcag gctgggcgag gatcgccggc atgtcggcgc
     3121 gcagcgcgcc gaggtcgagc gagcggatca tcgccttcac cgggcc
//
LOCUS       DATMSU010000448         3880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_140162,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000448 DATMSU010000000
VERSION     DATMSU010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140162"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..484)
                     /locus_tag="VN034_12035"
     CDS             complement(59..484)
                     /locus_tag="VN034_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWV61381.1"
                     /translation="MTIGAILRERTGAVISARPGDAVRTAVDLLAQNRIGAVPVLEGD
                     SVVGIFSERDIVRLLSSYGSDALDRSLDEVMTKSPVTCDANMAVIGALSQMTQKRIRH
                     LPVVDAGRLVGFVSIGDLVKYRIDRIEAEAAAMRDYIAS"
     gene            610..906
                     /locus_tag="VN034_12040"
     CDS             610..906
                     /locus_tag="VN034_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61382.1"
                     /translation="MVKRSPLDNRDTSNVAWARYRRLMKGMALVSLLAVLGALGWLRF
                     TMGEALTVHMIIATAAGVGLSVMLGAALMGLVFLSSGSGHDESIEDPFEDDPDF"
     gene            1081..1473
                     /locus_tag="VN034_12045"
     CDS             1081..1473
                     /locus_tag="VN034_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWV61383.1"
                     /translation="MTETTPPDCPRDPILRIVPRPADINANGHIFGGWVLSQMDIAGG
                     IVAGRVAQGAVATVAIETMEFIAPILLRDIISVYAHLERRGRTSMGIRIEVIATRDGG
                     RTEERVTEGLFTFVALDENHRPRALPPV"
     gene            complement(1619..3175)
                     /locus_tag="VN034_12050"
     CDS             complement(1619..3175)
                     /locus_tag="VN034_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61384.1"
                     /translation="MRRWPLLLSMLAASPAAAQPAVTSLAPEKVSVSVFRDPGRDEGG
                     EISPNWLEGFALISETRTIDLPAGEATVRFEGVAEGMIAVSAIVTGLPGGVVQKNRDA
                     ALLSPASLLDGSLGNRVHIRRTDRATGKVTEEEAIIRSGPAGAVVLQTSAGYEALRCT
                     GIPETILYDGVPAGLTAKPTLSVTTRSPAAQRATVTLTYLSTGFDWASNYVARVREDG
                     RTLDLFAWLTVANSNGESFADAGLAAIAGTLSKVSDFEELADRPRTPPLHLSCFPTGS
                     GRYGAPPPLPLLPAPPPPAAMEDGMDSIVVTGSRLQRSAMMSPVAVVASQEDLGDLKL
                     YRVPIPVTVAANGQKQVALLVKDDVPFRTIYRLRVYPGDEGKAMEPEILLRMQNKEAE
                     GLGVPLPSGQAAVFEPVGGHEMLVGEGRMRDHAVGEKVDLVIGESSQVRIDVENYVPP
                     KNRQQDYRITVTNANPHPADVELGFEVDDNDDLDSRIRKLPRRDGLLTWVVRVPANGS
                     KTFHYRVKDE"
     gene            complement(3189..>3880)
                     /locus_tag="VN034_12055"
     CDS             complement(3189..>3880)
                     /locus_tag="VN034_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HWV61385.1"
                     /translation="SRPYRPRPQPSGDLRRAGTETAPREQLGDYYLYPLAERTTIANA
                     QTKQVSFLDVSGVPAQKIYEYTVGGFDTMTQPASAASVIKFNTSAKGGGLGDALPAGT
                     VRFYQRDMRGDPQFIGENNIGHTPMGSELGLATGEAFDVKVQATVVKRERISDHRWRT
                     QMSYLLTNARANPVTLDLVQRGLDWYWDDTRIFDESRKSERLDSDGTRWRVEVPANGE
                     ATITATFETRY"
BASE COUNT          629 a         1296 c         1270 g          685 t
ORIGIN      
        1 tcgccgaagc cgttcgcctg gtggtccgcc gccgcgcgcc cgccgccgac gcgccgactc
       61 aggacgcgat atagtcgcgc atcgccgcgg cctcggcctc gatcctgtcg atgcgatatt
      121 tgacgaggtc gccgatcgac acgaacccga cgagtcggcc ggcatcgacg acgggcaggt
      181 ggcggatacg tttttgcgtc atctgcgaca gcgcgccgat caccgccatg ttcgcgtcgc
      241 aggtcaccgg cgatttggtc atcacctcgt cgaggctgcg gtcgagcgcg tcggaaccgt
      301 aggaggagag aaggcgaaca atgtcgcgct cggaaaagat gccgaccacc gaatcgccct
      361 cgagcaccgg aaccgcgccg attcgattct gcgccagcag gtcgaccgcg gtgcgaaccg
      421 catcgcccgg ccgcgccgaa atcaccgccc ccgttcgttc acgaagaatc gctccgatcg
      481 tcatagccgc cgctcccata tttggttctg ttgccgggca agcctatcat gattcgccgg
      541 tgggacgaaa cgtcccaccc gccgcaatgc cgcttggccg cggcggcggc ttggtccatg
      601 gaggcccgca tggtcaaacg ctccccgctc gacaatcgcg acacgtcgaa cgtcgcctgg
      661 gcgcgctatc gccggctgat gaagggcatg gcgctggtgt cgctgctcgc cgttctcggc
      721 gcgctcggct ggctgcgctt cacgatgggc gaagcgctga cggtccatat gatcatcgcg
      781 accgcggccg gcgtcggcct gtcggtgatg ctcggcgcgg cgctgatggg actcgtcttc
      841 ctgtcgagcg gcagcggcca tgacgaatcg atcgaagacc cttttgaaga tgacccggat
      901 ttctagggtc cagattcact atctacgggt tcgaggtttg aagcgatttt gttcagggct
      961 aggaggcgag gcgcaggcat atgattatat tccaaggctc gccgacgcag ccatggacaa
     1021 aatcgatcaa atcccggagg gacgcctcga acccgtagat agtgaatctg gacccttgcc
     1081 atgaccgaaa ccacgccccc cgattgcccg cgcgacccga tcttgcgcat cgtcccccgc
     1141 cccgccgaca tcaatgccaa cgggcatatc ttcggcggct gggtgctgag ccagatggac
     1201 atcgcgggcg gcatcgtcgc cgggcgcgtc gcccaaggcg cggtcgcgac ggtggcgatc
     1261 gagacgatgg agttcatcgc gccgatcctg ctgcgcgaca tcatctcggt ctatgcgcat
     1321 ctcgagcggc gcgggcgcac ctcgatgggc atcagaatcg aagtgatcgc gacgcgcgac
     1381 ggcggacgga ccgaggagcg cgttaccgag ggtctgttca ccttcgtcgc gctcgacgag
     1441 aatcaccgcc cgcgcgcgct gccgccggtt tagggcgtcg agcttataac ccgacccact
     1501 gttccgttcg tgtcgagcga agtcgagaca cccatcggcc ttgcgccttg ctgatgggtg
     1561 tctcgacttc gctcgacacg aacggattca agatagcgta aattgaaggc atagcgccct
     1621 actcatcctt cacccggtaa tggaacgtct tgctgccgtt cgcgggaacg cgaacgaccc
     1681 atgtcagcaa gccgtcgcgg cgcggaagct tgcggatgcg cgaatcgagg tcgtcattgt
     1741 cgtcgacctc gaagccgagc tccacatccg ccggatgcgg gttcgcattg gtcacggtga
     1801 tgcgatagtc ctgctggcga ttcttcggcg gcacataatt ttcgacgtcg attcgcactt
     1861 ggctgctctc gccgatgacg agatcgactt tctcgccgac cgcatggtcg cgcatccggc
     1921 cctcgccgac gagcatctcg tggccgccga cgggctcgaa caccgccgcc tgtccgctcg
     1981 gcagcgggac gcccagccct tcagcctcct tattctgcat ccgcagcagg atttcgggct
     2041 ccatcgcctt cccctcgtcg cccggataga cgcgcaggcg atagatggtg cggaagggca
     2101 catcgtcctt gacgagcagc gcgacctgtt tctggccgtt ggcggcgacg gtgaccggga
     2161 tgggcacgcg atagagtttg aggtcgccca gatcctcctg actcgcgaca accgcgacgg
     2221 gcgacatcat cgccgagcgc tgcaggcgcg atccggtgac gacgatgctg tccatgccgt
     2281 cctccatcgc cgcgggcggc ggcggcgcgg gcagcagcgg aagtggaggc ggagcgccat
     2341 agcgtccgct gccggtcgga aagcagctga gatgcagcgg cggcgtgcgg gggcggtcgg
     2401 cgagttcctc gaaatcgctg accttgctca gcgtgcccgc gatcgccgcg agcccggcgt
     2461 cggcgaaact ctcgccattg ctgttggcga ccgtcagcca ggcgaacagg tcgagcgttc
     2521 tcccgtcctc gcgcacgcgc gcgacataat tgctcgccca gtcgaagccg gtcgacagat
     2581 aagtcagcgt cacggtggcg cgctgcgccg ccgggctgcg ggtcgtcacc gacagcgtcg
     2641 gcttcgcggt cagccccgcg ggcacgccgt catagaggat cgtctcggga atgccggtgc
     2701 agcgcaacgc ttcgtagccc gccgatgtct gcagcacgac ggcccccgcc ggacccgagc
     2761 ggatgatcgc ttcctcctcg gtgaccttgc ccgtcgcgcg atcggtgcgg cggatatgga
     2821 cgcggttgcc gagcgagccg tcgagcaggc tggcgggcga cagcagcgcc gcgtcgcgat
     2881 ttttctgcac caccccgcca ggcaggccgg tgacgatcgc cgacaccgcg atcatcccct
     2941 cggcaacgcc ttcgaatcgc accgtcgcct cgcccgccgg caggtcgatc gtccgcgtct
     3001 cggagatcag cgcgaaaccc tcgagccagt tgggcgaaat ctcgccgcct tcatcgcgac
     3061 cgggatcgcg aaagacgctg accgagactt tctcgggggc aagcgaggtc accgcgggct
     3121 gcgccgccgc cggggacgcg gcgagcatcg acagcaggag cggccagcgg cgcatcgtcg
     3181 gccgcgaatc agtatcgtgt ttcgaaggtg gcggtgatcg tcgcttcgcc attggcgggc
     3241 acttcgaccc gccaccgcgt gccgtcgctg tcgagccgct cgctcttccg gctttcgtcg
     3301 aagatgcgcg tgtcgtccca ataccagtcg agcccgcgct ggacgaggtc gagcgtcacc
     3361 ggattcgccc gcgcattggt gagcagatag gacatttgcg tccgccagcg atggtccgaa
     3421 atgcgctcgc gcttcacgac cgtcgcctgc accttgacgt cgaatgcctc tccggtcgcg
     3481 aggccgagct cgcttcccat cggggtatgg ccgatgttgt tttcgccgat gaactgcgga
     3541 tcgccgcgca tgtcgcgctg atagaagcgc accgttcccg cgggcagcgc gtcgccgagg
     3601 ccgccgccct tcgcgctcgt gttgaacttg atcacgctcg ccgcgctggc gggctgggtc
     3661 atcgtatcga acccgccgac ggtatattca tagattttct gcgccgggac gccgctgacg
     3721 tcgaggaagc tcacctgctt ggtctgcgcg ttggcgatcg tcgtccgctc ggcgagcgga
     3781 tagagataat aatcgccgag ctgctcgcgc ggcgcggttt cggtcccggc acggcggagg
     3841 tcgccggacg gctgcgggcg cgggcgatag ggccgggaac
//
LOCUS       DATMSU010000449         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_26405, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000449 DATMSU010000000
VERSION     DATMSU010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26405"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..2107
                     /locus_tag="VN034_12060"
     CDS             59..2107
                     /locus_tag="VN034_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HWV61386.1"
                     /translation="MMRSLFVALPLLAAAVAPAAAQETRPEQTAASPDGSIVLTVSTD
                     NDQRPTWSLTRKGKLLIAPSKLGFLLTDGIALQRGFAIESVERASGDTRWEQPWGERR
                     FVRDRHNELLVRFRQNESWGGHAMNIRFRLFDDGIGFRYELPEQAGLQTAKIADEITE
                     FDIVPKGTAWWITGGEWNRYEQIYQQTPIDAVSTAHTPITMRLDDGTHLAFHEAALVD
                     YAGMWFKRVEGRKFRATLSPSSQGPRVIRDLPFATPWRTIRIAGTAAGIVESDLELNL
                     NEPNKLGDVSWFKPMKYIGIWWGMIRGPWSWAEGPQHGATTERTKQYIDFAAKHGFGG
                     VLVEGWNKGWNGNWFGHGDEFSFTEAYPDFDLKAVTDYARKKGVTLIGHHETGGNIAV
                     YEAQLDDAMKLYGELGVKAVKTGYVADAGGIIAPGDTPGETRMEWHDGQRQVQHHLKV
                     VETAAKYHVAVNPHEPIKDTGLRRTYPNWVAREGARGMEYNAWSDFGSGPDHEPTLVY
                     TRMLSGPMDYTPGVLSLEGSNKAPFASTLAKQLGLYLAIYSPIQMAADFIENLAAHPR
                     ELEFIKMVPADWSESKLIAGEVGDYAIFARKDRKSADWYVGGVNDATARTLTLDFDFL
                     DPGKTYTATIWKDGEGATYLTDARHRIAYETIKVKKGDTYKLWLAPGGGAAMRVAPGK
                     "
     gene            complement(2265..>3269)
                     /locus_tag="VN034_12065"
     CDS             complement(2265..>3269)
                     /locus_tag="VN034_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWV61387.1"
                     /translation="LFALTTAIAVSVGMAVALLVEPGVAAPVGTAAPKALGDPVPLYD
                     QLVGIIPLNIVEALAKGDMLALIFVAILVGVGTVLTGEAGKPFAGLLQSLSAVLLKIV
                     GLVMEATPIGVFALIANAVAANGAAVFVNIGWLALAVVLGSAIQIILVHSLILRLLAR
                     LPVLTFFRGIVDALVVAFSTASSSATLPVAMTVAGKNLGISPSVYSTVLPLGASIGKD
                     GTAMYVGLLSIFSLQAFGVPIDAGVLAIVLLTGALAAFGTAPVPSASLFMLAAVLSAV
                     GVPPEQTALIVGFVLPFDRLLDMTRTVPSASANLTVATTVARWEGELDQSIYRAPPRR
                     "
BASE COUNT          660 a         1063 c         1073 g          473 t
ORIGIN      
        1 gccgacccgg cgcacttgcc gccgtgcggc gccctctggc tgaaactcga aggaaataat
       61 gatgcgtagc ctgttcgtcg ccctcccgct gctcgccgcc gcggtagccc ccgccgcggc
      121 gcaggaaacg cggccggagc agaccgccgc atcgcccgac ggcagcatcg tcctcaccgt
      181 gtcgaccgac aacgaccaac ggccgacctg gtcgctgaca cgcaagggca agctgctgat
      241 cgcgccgtcg aagctcggct tcctgctcac cgacggcatc gcgctccagc gcggcttcgc
      301 gatcgaaagc gtcgagcgcg cgagcggcga cacgcgctgg gaacagccgt ggggcgaacg
      361 ccgcttcgtg cgcgaccgcc acaacgagct gctcgtccgc ttccgccaga acgaaagctg
      421 gggcggccat gcgatgaaca tacgcttccg cctgttcgac gacggcatcg gcttccgcta
      481 tgaactgccc gaacaggcgg ggctccagac cgcgaagatc gccgacgaaa tcaccgaatt
      541 cgacatcgtg ccaaagggca ccgcctggtg gatcaccggc ggcgaatgga accgctacga
      601 gcagatctat cagcagacgc cgatcgacgc ggtgtcgacc gcacacacgc cgatcacgat
      661 gcgcctcgac gacggcacgc atctcgcgtt ccacgaagcc gcgctcgtcg actatgcggg
      721 catgtggttc aagcgcgtcg aggggcggaa gttccgcgcc accctgtcgc cttcgtcgca
      781 ggggccgcgt gtcatccgcg acctgccgtt cgcgaccccg tggcggacga tccgcatcgc
      841 gggcaccgcc gccgggatcg tcgaaagcga cctcgaactc aacctcaacg agcccaacaa
      901 gctcggcgat gtcagctggt tcaagccgat gaaatatatc ggcatctggt gggggatgat
      961 ccgcggccca tggagctggg ccgaaggccc gcagcatggc gcgacgaccg aacgcaccaa
     1021 acaatatatc gacttcgcgg cgaagcacgg cttcggcggc gtgcttgtcg agggctggaa
     1081 caagggctgg aacggcaatt ggttcggcca tggcgacgaa ttcagcttta ccgaagccta
     1141 tcccgatttc gacctgaagg cggtcaccga ttacgcgcga aagaagggcg tgacgctgat
     1201 cggccatcat gaaaccggcg gcaatatcgc ggtatacgag gcgcagctcg acgatgcgat
     1261 gaagctctat ggcgagcttg gcgtcaaggc ggtgaagacc ggttatgtcg ccgacgccgg
     1321 cgggatcatc gcgccgggcg acacgccggg cgaaacgcgc atggaatggc acgacggcca
     1381 gcggcaggtg cagcatcatc tgaaggtcgt cgaaaccgcc gccaaatatc atgtcgcggt
     1441 caatccgcac gaaccgatca aggacacggg cctccgccgc acctatccga actgggttgc
     1501 gcgcgagggt gcgcgcggca tggaatataa tgcctggagc gatttcggca gcggcccaga
     1561 ccatgaaccg acgctcgtct acacgcgcat gctctcggga ccgatggact atacgccggg
     1621 ggttctgagc ctcgaaggct ccaacaaagc acccttcgca tcgacgctcg ccaagcagct
     1681 cggcctctat ctcgccatct attcgccgat ccagatggcg gcggatttca tcgagaatct
     1741 ggcggcgcat ccgcgcgagc tcgagttcat caagatggtc ccggccgact ggtccgaaag
     1801 caagctgatc gcgggcgagg tcggcgatta tgcgatcttc gcacgcaaag accgcaaaag
     1861 cgccgactgg tatgtcggcg gggtcaacga cgcgaccgcg cgcacgctga cgctcgattt
     1921 cgatttcctc gatcccggca agacctatac cgcgacgatc tggaaggacg gcgagggcgc
     1981 cacctatctc accgacgcgc ggcaccgcat cgcttatgag acgatcaagg tgaaaaaggg
     2041 cgacacctat aaattatggc tggcgccggg cggcggcgcc gcgatgcgcg tcgcgccggg
     2101 gaagtagccc gaaagacctt aacgcggtca tttattgtcg tcatcccggc gaaggccggg
     2161 atctcgccgt ctcgacctga cgcactggcg agatcgtgtt tcagagtcac gtgcctctga
     2221 acacccttcg ccgggatgac gattcaatta agaatgatca cgccctaacg ccggggcggc
     2281 gcgcgataga tcgactggtc gagctcgcct tcccaacgcg ccacggtggt cgcgacggtc
     2341 agattggcgc tcgcgcttgg caccgtgcgc gtcatgtcga gcaaccggtc gaagggaagc
     2401 acgaagccga cgatcagcgc ggtctgttcg ggcggaacgc cgaccgccga aagcaccgcc
     2461 gcgagcatga agagcgacgc cgacggaacg ggggcggtgc cgaaggcggc gagcgcgccg
     2521 gtcagcagca cgatcgcgag cacgccggca tcgatcggca cgccgaacgc ctgcaggctg
     2581 aatatgctga gcagcccgac atacatggcg gtgccgtcct tgccgatgct cgcgccgagc
     2641 ggcagcacgg tcgaatagac gctgggcgat atgccgagat tcttgcccgc gacggtcatc
     2701 gcgacgggca gggtcgccga tgacgaggcg gtcgagaaag ctacgacgag cgcgtcgacg
     2761 atgccgcgaa agaaggtgag gacgggcagg cgggcgagca gccgcaggat gaggctgtgg
     2821 acgaggatga tctggatcgc cgaccccagg acgacggcga gcgccaacca gccgatattg
     2881 acgaaaaccg cggcgccatt ggcggcgacg gcattggcga tcagcgcgaa tacgccgatc
     2941 ggggtcgcct ccatcaccag tccgacgatc ttcagcagca ccgccgacag cgactgcaac
     3001 aggccggcga acggcttgcc cgcctcgccg gtgagcacgg tgccgacgcc gacgaggatc
     3061 gcgacgaaga tcagcgcgag catatcaccc ttcgcgagcg cctcgacgat gttgagcggg
     3121 atgatgccga caagctggtc atagagcggc accgggtcgc ccagcgcctt gggcgctgcg
     3181 gtgccgacgg gggcggcgac gcccggttcg acgagcagcg cgaccgccat gccgaccgac
     3241 accgcgatcg ccgtcgtcag cgcgaacag
//
LOCUS       DATMSU010000450         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_55085, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000450 DATMSU010000000
VERSION     DATMSU010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55085"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..873)
                     /locus_tag="VN034_12070"
     CDS             complement(73..873)
                     /locus_tag="VN034_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWV61388.1"
                     /translation="MTTSPSALDALLATLRTEDGVAKAHIDEGWMQGRTAYGGISSAV
                     ALAGTMALHPTETPLRYAQISFVGPVGGDCTVETRVLRQSKSSLFIDAGVSSDQGFGT
                     AAVFAFSGDRTSHVDHNRLAMPDAPDPETLAPVPEHHARPSFTRHFDMRPTTGPRFAW
                     KSEVGEYLTWVRFVEEPACHPAVALLAMGDALPPAAMALFSQFGPISSMNWTVNMLTG
                     TPATDDGWWLLSAKTGYARHGLSVQDMMLWNRAGEPVLSGSQAIAIYA"
     gene            complement(881..2050)
                     /locus_tag="VN034_12075"
     CDS             complement(881..2050)
                     /locus_tag="VN034_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWV61389.1"
                     /translation="MASRDFDIVVYGATGFTGRLVAEYLAHHYKGRKDAPKWAMAGRS
                     LDKLAEVRGLIGASPETPLIVADASDPASLDKMAQSTKVVLTTVGPYQLYGSELVAAC
                     VRAGTAYADLCGEPGWMREMIDEHEAAAKASGARITFSCGFDSIPFDLGVSFLQAEAV
                     KRHGKPAPRVKGRVRKMAGGASGGTIASLTETLKAIAKKPSLALLLKSSFALTPGFEG
                     PSQPTGLIPEYDAATGTWTAPFVMAPINTKNVHRTNFLLGHKWGANLVYDEMVMTTIG
                     DAGKAMAEAMAKANPFGESTLQPGEGPSKEEREKGFYDILFIGEYPDGTTVRASVQGD
                     RDPGYGSTSKMLAETGIALLENKGAGGVWTPGALLGDELIERLTANAGLTFQIEE"
BASE COUNT          346 a          710 c          745 g          383 t
ORIGIN      
        1 aaggcggagg ccgctggagg cgtagcgcca gtccgacagc ggcccccgcc ttcgcggggg
       61 cgacggataa atttatgcgt agatagcaat cgcctggctg ccgctgagga ccggttcgcc
      121 ggcgcggttc cacagcatca tgtcctgcac cgacaggccg tggcgcgcgt agccggtctt
      181 ggcggacagc agccaccagc cgtcgtcggt ggcgggcgtc cccgtcagca tgttgaccgt
      241 ccagttcatc gagctgatcg ggccgaactg gctgaacagc gccatcgcgg cgggcggcag
      301 cgcgtcgccc atcgcgagca gcgcgaccgc gggatggcac gctggctcct cgacgaagcg
      361 cacccaggtc agatattcgc cgacctcgct tttccaggca aagcgcgggc cggtcgtggg
      421 gcgcatgtcg aaatggcgcg taaaggacgg gcgcgcatga tgttcgggca ccggcgccag
      481 cgtttcggga tcgggcgcat cgggcatcgc cagccggtta tggtcgacat ggctcgtccg
      541 atcgcccgaa aaggcgaaga ccgcggcggt gccgaacccc tgatcgctcg acacgccggc
      601 gtcgatgaac agcgaggatt tcgactgccg cagcacgcgc gtctcgacgg tgcagtcgcc
      661 cccgacgggg ccgacgaagc tgatctgcgc atagcgcagc ggtgtctcgg tcggatgcag
      721 cgccatcgtg cccgcaagcg cgaccgccga gctgatcccg ccataggccg tgcgcccctg
      781 catccatcct tcgtcgatat gcgccttcgc caccccgtct tcggtgcgca gcgtggcgag
      841 cagcgcgtcg agcgcgctgg gggaggtcgt cattctgggc ttattcctca atctgaaagg
      901 tcagtccggc attagcggtc aatcgctcga tcagttcgtc gccgagcagc gcgcccggcg
      961 tccacacgcc gcccgcgccc ttgttttcga gcagcgcgat gcccgtttcg gcgagcatct
     1021 tcgaggtcga gccatagccg gggtcgcggt cgccctgaac gctcgcgcgg acggtggtgc
     1081 cgtcgggata ttcgccgatg aagagaatgt cgtagaagcc cttctcgcgc tcttccttgc
     1141 tcgggccctc gccgggctgg agcgtcgact cgccaaaggg attcgccttc gccatcgctt
     1201 cggccatcgc ctttccggcg tcgccgatcg tcgtcatcac catctcgtcg taaaccagat
     1261 tcgcgcccca cttgtggccg agcaggaaat tggtgcgatg gacattcttg gtgttgatcg
     1321 gcgccatcac gaagggcgcg gtccaggtgc cggtcgccgc gtcatattcg gggatcagcc
     1381 ccgtcggctg cgaagggccc tcgaaccccg gggtgagcgc gaaggaggat ttgagcagca
     1441 gcgcgagcga cggcttcttg gcgatcgctt tcaatgtttc ggtcaggctg gcgatcgtgc
     1501 cgcccgaggc tccgcccgcc atcttgcgga cgcggccctt cacccgcggc gcgggtttgc
     1561 cgtggcgttt gaccgcctcg gcctggagga aggagacgcc gaggtcgaag gggatcgagt
     1621 cgaagccgca agagaaggtg atgcgcgcgc ccgacgcttt ggccgcggct tcatgttcgt
     1681 cgatcatctc gcgcatccag ccgggttcgc cgcagagatc ggcataggcg gtgcccgcac
     1741 ggacgcaggc ggcgacgagt tcgctgccgt aaagctggta ggggccgacc gtcgtgagca
     1801 cgaccttcgt gctttgcgcc atcttgtcga ggctcgcggg atcgctcgcg tcggcgacga
     1861 tcagcggcgt ttcggggctc gcgccgatga ggccgcgaac ttcggccagc ttgtcgaggc
     1921 tgcggcccgc catcgcccat ttgggcgcgt ccttgcggcc tttgtaatgg tgcgcgagat
     1981 attcggcgac gaggcggccg gtaaagcccg tcgcgccgta aacgacgatg tcgaaatcgc
     2041 gggacgccat ggcaaaccct ccctttacgt aaacgttaag tggaggctag cgcagacggc
     2101 ccgcgatgcc aagcgcgaag gttagagcct aacctattca ggctctagcg gcgccagaag
     2161 gggcggcgct tttcgacgac cacc
//
LOCUS       DATMSU010000451         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_46025, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000451 DATMSU010000000
VERSION     DATMSU010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46025"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VN034_12080"
     CDS             complement(<1..329)
                     /locus_tag="VN034_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWV61390.1"
                     /translation="MTLLLASQSSGRAAMLRAAGLTFETSAAHVDEEALTASLLAAGQ
                     TPRNIADALAEAKAVKISSRLPGVTVIGADSTLALDDGAMLAKPASPEDAADHLRRMA
                     GTRHRLF"
     gene            complement(326..1138)
                     /locus_tag="VN034_12085"
     CDS             complement(326..1138)
                     /locus_tag="VN034_12085"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HWV61391.1"
                     /translation="MGRLHLHLISDSTGETLENIAKAAIAQFDDVEVVRHFWPMVRSE
                     SHLDRIMAEVQASPGMILFTLVNGELRVSLERRARALNLPTVAALDAVTDALSRMLGQ
                     EAKARPGRQHVLDAAYFARVEAIQFTVAHDDGIGWENWEQADIVLAGVSRTSKTPTSI
                     YLANRGFKTANIPIVPESPPPNALFNLKRPMIVGLTTGLDRLVQVRRNRLLSLNQAPE
                     TSYVDDERVKAELAYARRMFADNGWPVIDVTRRSIEETAAAVIKLVEDRAGA"
     gene            1487..2515
                     /gene="hemE"
                     /locus_tag="VN034_12090"
     CDS             1487..2515
                     /gene="hemE"
                     /locus_tag="VN034_12090"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWV61392.1"
                     /translation="MKASPKTLLATLRGVRQERPPLWLMRQAGRYLPEYRALRETKGG
                     FLELCYDPEAAAEVTLQPIRRFGFDGSILFSDILVIPHALGQHLWFEAGEGPRLAPPL
                     IDSALASLEAAPERLDPVYATVARVAAALPPETTFLGFAGSPWTVATYMVAGQGSKDQ
                     AAARRMAFGDPAAFEAIIDAIVDLTVTYLAGQIENGVDAVQLFDSWAGSLSPGQYEQW
                     VIAPNAEIVRRLRKLHPDTPVIGFPKGAGGKLRAYADETGVDAIGLDETVDPAWADAA
                     LSPHLPVQGNLDPLALVAGGAALDTAIDRILAAFPDRPHIFNLGHGIVPDTPIAHVEH
                     LIKRVRGG"
     gene            2549..2965
                     /locus_tag="VN034_12095"
     CDS             2549..2965
                     /locus_tag="VN034_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWV61393.1"
                     /translation="MLWIKAAHIIFVIFLMAGLFMMPRFFVYHHQAPVGSDEDRKWIE
                     REDRLRRIILGPSLIIVWIFGLMLAFNGDYWREGWFIAKLLLVIALSGYHGWMIGYFK
                     KLKKGERPLSEKQLRMLNEVPGIAAAIIVILVVVRP"
     gene            3189..>3586
                     /locus_tag="VN034_12100"
     CDS             3189..>3586
                     /locus_tag="VN034_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HWV61394.1"
                     /translation="MHLKELKTKAPAQLVEMAEELGVEGASTLRKQDLMFSILKELAE
                     EGEEIIGSGTIEVLPDSFGFLRSSDANYLAGPDDIYVSPNQVRKYGLRTGDTVEGEIR
                     APRDGERYFALTKLIKVNFDDPEAVRHRVN"
BASE COUNT          636 a         1126 c         1158 g          666 t
ORIGIN      
        1 ctgaacaggc ggtggcgggt tcccgccata cggcggagat gatcggcggc gtcctcgggg
       61 cttgcgggtt tcgcgagcat cgcgccatcg tcgagcgcga gggtgctgtc tgcacctatc
      121 actgtcacac ccggaagccg cgacgagatt ttaacggctt tggcttctgc gagcgcgtcg
      181 gcgatgttgc gcggcgtctg cccggcggcg agcaaggaag cggtgagcgc ctcctcatcg
      241 acatgtgcgg cgctggtttc gaaggtcagc ccggcggcgc ggagcatcgc ggcgcggccg
      301 ctgctttgcg aggcgaggag gagtgtcatg cgccggcccg gtcttcgacg agcttgatca
      361 ccgcggcggc ggtttcctcg atcgagcggc gcgtgacgtc aatcaccggc cagccattgt
      421 cggcgaacat ccgccgcgca taggcgagtt cggctttcac gcgttcgtcg tcgacgtacg
      481 aggtttcggg cgcctgattc agcgagagca gccggttgcg gcgcacctgg acgagccggt
      541 cgagcccggt cgtcagcccg acgatcattg gccgtttcaa gttgaacagc gcgttcggcg
      601 gcggcgattc gggaacgatc gggatattcg cggtcttgaa gccgcggttc gcgagataga
      661 tgctcgtcgg cgttttcgac gtgcgcgaca caccggcgag gacgatgtcg gcctgttccc
      721 aattttccca gccaataccg tcgtcgtgcg cgacggtgaa ttgaatcgcc tcgacgcgcg
      781 cgaaataggc ggcgtcgagg acgtgctggc ggccgggacg cgccttcgcc tcctgcccga
      841 gcatccgcga caacgcgtcg gtcaccgcgt cgagcgcggc aacggttggc agattaagag
      901 cgcgcgcgcg gcgttcgagg ctgacgcgga gttcgccgtt gacgagggtg aagaggatca
      961 tgccggggct ggcctgcact tcggccatga tgcggtcgag atgcgattcc gatcgcacca
     1021 tcggccagaa atggcgcacc acttcgacat cgtcgaactg cgcgatcgcc gccttggcga
     1081 tattttcgag cgtctcgccc gtggaatcgg atatgaggtg aaggtgaagc cggcccatcg
     1141 cgccaccatg gcgggaaacc gcgaaaaaag gcaatgccgg atcgcgcccg gcatggcgaa
     1201 aagccccctg tggataagtt tggcaaaagc gccgggatga tttcggcacg aaggattcat
     1261 cccctgccgt gtcgattccg ctccaccgct tatccacacg ggaggaatct tatccacagg
     1321 ctgtgaatta gggggacagc gcgacgcgac tccctagcgt gacccgcgat acctcgagtc
     1381 aagctgttgg caaaaaggac gccccggcct aaagccgcct tgtccgccga cctacaaacc
     1441 accacaatca tatttaaata tatatgaaga gagaaagaag ggccgcgtga aggcgtcacc
     1501 gaagacactg ctcgcgacgc tgcgcggggt gcggcaggag cgcccgccgc tctggctgat
     1561 gcgtcaggcc gggcgctatc tgcccgaata tcgcgcgctt cgtgagacca agggcggttt
     1621 cctcgagttg tgctacgatc ccgaggcggc cgccgaagtg acgttgcagc cgatccggcg
     1681 cttcggcttc gatgggtcga tcctcttttc cgacattctc gtcatcccgc acgcactcgg
     1741 ccagcatctg tggttcgagg cgggcgaagg accgcggctc gctccgccgc tcatcgacag
     1801 cgccctggcg tcactcgagg cggcgcccga gcggctcgac ccggtttacg cgaccgtcgc
     1861 gcgcgtcgcg gcggcgctgc cgcccgagac gacctttctg ggcttcgcgg gaagcccgtg
     1921 gacggtcgcg acctatatgg tggcggggca ggggtcgaag gaccaagccg cggcgcggcg
     1981 catggcgttc ggcgatcccg cggcgttcga ggcgatcatc gatgcgatcg tcgatctgac
     2041 cgtgacctat ttggcggggc agatcgaaaa tggcgtcgac gccgtgcagc ttttcgacag
     2101 ctgggcgggc agcctgtcgc cggggcaata tgagcaatgg gtgatcgcgc ccaatgccga
     2161 aatcgtacgc cgcttgcgca agctccatcc cgacacgccg gtcatcggct ttccaaaggg
     2221 tgccgggggc aagctccgcg cttatgccga cgagaccggg gtcgatgcga tcggcctcga
     2281 cgagacggtc gatcccgcct gggccgacgc cgccttgtcg ccgcatctgc cggtgcaggg
     2341 caacctcgat ccgctcgcgc tcgtcgcggg cggcgccgcg ctcgatacgg cgatcgaccg
     2401 catccttgcg gctttccccg atcgtcccca tatcttcaat ctcggccatg gcatcgtgcc
     2461 cgacacgccg atcgcgcatg tcgagcatct gatcaaacgc gttcgcggcg gataatctat
     2521 ggaactggca ggttttttgg gggtgacgat gctttggatc aaagccgcgc atatcatttt
     2581 cgtgatcttc ctgatggccg gcctgttcat gatgccgcgc ttcttcgtct atcatcatca
     2641 ggcgcccgtc gggtccgacg aggacaggaa gtggatcgag cgcgaagacc ggttgcggcg
     2701 gatcatcctg ggtccgtcgc tgatcatcgt ctggatcttt ggcctgatgc tcgccttcaa
     2761 cggcgattat tggcgcgagg gctggttcat cgccaaattg ctgcttgtga tcgcgctgtc
     2821 gggctatcac ggctggatga tcggctattt caagaagctc aagaagggcg agcgtccgct
     2881 cagcgaaaaa cagctcagga tgctcaacga ggtgcccggc atcgccgcgg cgatcatcgt
     2941 catactcgtg gtggtgcgcc cctgacccca gagtctttcc gggcccggaa agactctact
     3001 cgccccggcg cagcccgatt gacttgatcc cgagccggtt ataggggcga ttcaaccccg
     3061 ttccacacgg gcggcgcgtc ccccgtgccg atgctatcga aacggtcgtc ccacggccgg
     3121 ataggcaccc cagcggtgcg acaaattcgc gtcctatttt tcccgaagac ctatctggaa
     3181 tccatccaat gcatctcaaa gaactcaaga ccaaggcgcc cgcccagctc gtcgaaatgg
     3241 cggaagaact gggcgtcgaa ggcgcttcga cgctgcgcaa gcaggacctg atgttctcga
     3301 tcctgaaaga actcgccgaa gagggcgagg aaatcatcgg gtcggggacg atcgaggttc
     3361 tccccgactc attcggcttc ctgcgctcgt cggacgccaa ttatctcgcc ggccccgacg
     3421 acatttacgt ctcgccgaac caggtccgca aatatggcct gcgcaccggc gacaccgtcg
     3481 agggcgagat tcgcgccccg cgcgacggcg aacgctattt cgcgctgacc aagctgatca
     3541 aggtcaattt cgacgacccc gaagcggtgc gccaccgcgt caattt
//
LOCUS       DATMSU010000452         8906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_178303,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000452 DATMSU010000000
VERSION     DATMSU010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8906
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178303"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VN034_12105"
     CDS             complement(<1..579)
                     /locus_tag="VN034_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61395.1"
                     /translation="MTFDVAAQADLRNHPLFKRAEDLTRAWLRPGTGEAALLGQLAAS
                     PDGRHVVAAASICDALEGLSSRDGGSTTRLVLVDLASGEMETLTHGPRSDSAPTWSPD
                     GGTIAFLSDREQAHITHLRLFDPENRTDRAAAAVDGFIEALQWSPDGKTILLSVAGYG
                     SDLAGAQGAMAIGLESGGAEQPGWAPAIEGAAE"
     gene            complement(576..2618)
                     /locus_tag="VN034_12110"
     CDS             complement(576..2618)
                     /locus_tag="VN034_12110"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWV61396.1"
                     /translation="MMQAKPVQKIIGGVAFEDRFAHLHEDTPEALDWQWERDRIARET
                     AEASPNYGPVHDRLLQLADASGYFVPRKRGASWFGYAEDGDDLVLRVSDQPNGPGRTI
                     VSKRAVSEAHGGGNVMLAFMEPSPRGRFVAVVWGVDGDMMGQWSVYEAATGRHVLDTP
                     AILYSGARPGWLPDESGFWLDGRNAEGLHELRFVPVADGAAGRPAVLLSESLVAAKHS
                     GLTLQVSPDGRRGVAVTEPHEHVALALLDLETLEPTPFLPDGFEGECDGSWIDGETYV
                     ARVNNGVPRGRVVAIPAASSRDMASWRELVPEGEGFIGWAGVVGGRLYVGDLVDVSLR
                     VRVFDLEGRLVDTLPFENPGSSQSMLTERAIRPSDAFAITHTTFTRSSVLFVHDPQTG
                     ELRLIGEAKHELGDAIAERRFATSRDGTRIPYFVVRRKDVDLGSAQPTLVYAYGGFNL
                     SQLPSFPATHVPFIEAGGVFVQASLRGGAEYGKAWHDGGRLFNKQNTFDDLEAVAEAL
                     IADGVSVPDKMIFMGGSNGGLLAGVAIVQQPHLWRAVVPTVPIFDMMEPLPDTPAVAG
                     VRAIFFEDYGDPNDPEHAASIRHWSPYHNVRDGVEYPAVYQVFGEKDLGCMPFHGRKF
                     TARLEEANAGDRPVHLRVWRDTGHGAHDPADAARWNGEWLAFAMDQIGLNAAGPGA"
     gene            complement(2665..3162)
                     /locus_tag="VN034_12115"
     CDS             complement(2665..3162)
                     /locus_tag="VN034_12115"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV61397.1"
                     /translation="MAEPIPVTMVNHVAMDMAAPPDEVWRVILDDYIEAKKFREVGAI
                     EPIDDPAAVFGGYRMRFEQDGVVDERIVHITERDEGARRLSAAADYLSVPGGMRVYAT
                     YHAQEAGEGTRYAIDCHTQLRIDAPAGDVKAGVAATLAEMTAGADMHLVAYLENIKAR
                     VETRG"
     gene            3331..4479
                     /locus_tag="VN034_12120"
     CDS             3331..4479
                     /locus_tag="VN034_12120"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWV61398.1"
                     /translation="MHHTGDAPSTAMPALSPGSGNRANRRALALPTRARRFPVAADRL
                     QPVVVDRKIHDIVVEQDRAKPDTGVETYASLGDPRHFRVQGSANGASGYYEYCALDEG
                     FFAVISDIIYSTPLPLSLAAENMVRVRIASDGDGEYAAADGDLLDLKGPSASIIIEPP
                     GQAPAEAVSAGHHLAAEIYVHRDVLARLYARDDHELPAVLQAFITGHLQHTIARRLPL
                     NPQLLRAMEDLRGCTLEGRSRRLVIRSKAIEILCHAIEALAQDENGDAPPEASKLAAN
                     GVLKAQRLLAENFVSPPSLDSLAHEVGMSRSGLCAAFRQIVGQSVFDYVGDLRMQQAL
                     AMLNRQDATIAEVAYAVGYGHPSSFSVAVQRRFGTTASELRRRGLPPA"
     gene            complement(4501..6213)
                     /locus_tag="VN034_12125"
     CDS             complement(4501..6213)
                     /locus_tag="VN034_12125"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWV61399.1"
                     /translation="MTLGPSAWPEAEREHYWNIQQTALPGNPPARGKGGAVSTALNGL
                     AARVGEETLRQGGSAVDAVMSSALAQIVLGGGAVISFFGILSLMHYDAASDDVTSLNA
                     GWNTIVAEDDPMSIPGKLNPAADGVTNMMLEGEPSGRTAMVGGFMRGLEAAHRRFGKL
                     PFARLFDAAIELAEDGFAVSAGLARYIDVRKKDLARLPETRSIFYKSDGTPYTEGDHF
                     RQPALAETLRRVASEGADYMYKGAWAARCVEAVQRDGGKMTLADLADYRPIWSTPATV
                     EREGHVIAVLGEPCEGSVNLIESLNLAEAAGIKDIGHWSTSSDSLVRLAKCCAAIGGM
                     RYESPEEQQKAFPGIDMSRSSRLTREHAGELWERLVDQAPVRFLPKGTHSDVVVAVDA
                     DGNMAALCHSINCLIWGQTAIVVDGVSVGDPAAYMQSMVAATPRGAQLPNPIEVGLVI
                     KDGQPEIAWSSMGVGLHYQTTQSLLNVIDFGMTIEEAADAPRLLLPISPDGSQRELIL
                     RVVEGEFPDELLGATGLEVKSIPPAEARFAQGLWVAVRRDPATGDLTAISPRYTNGQA
                     AAIA"
     gene            complement(6236..8185)
                     /locus_tag="VN034_12130"
     CDS             complement(6236..8185)
                     /locus_tag="VN034_12130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWV61400.1"
                     /translation="MIERDMRGSAAMREVEALYRTLFYPGEEHLWTATDLDPAPDGQS
                     VHFSAQSFEGTLADGPSSWICRLDLASANVRRIARGRLYRSAPDGRAAHVADGDGQER
                     LVIMSDSGERIAAIALDMRVEALAWSPGGRDLLILGADSGADVSGAEGGFALRSDAGG
                     PAWLPEVASSGAQNLWRRLYVWTAGAMTLRVVAQPPVNVWEAAWLGDDAFAVIASDHH
                     SEGSWYEASLRILDAGTGAERARYTGAEQIGVPAGSPDGNSWAILEAFCSDRGIICGP
                     ITLGDAHGERVLDTGGVEVTDLRWRDPDHLLFAGVRDAETVVGEINVATGERLEHWAS
                     DTLTIGGWYPSAVPGADGSVLAVVEGYARPPAIARLSNGEAEVFADFAPPSPPAIPGA
                     MRYHRWQGRDGLEIGGWLVLPEGEAKDLPLFVDIHGGPVWAHRNRYAAALRAGPVLVG
                     KGYALLLPNPRGSGGRGQDFARRVHKDMGGEDMHDYLTGIESLVQAGIVDATRVAVSG
                     TSYGGFMSSWLVTQTDRFAAAVPISPVANWYSQHYASQIPWFDAALLEGSPRRPGGQY
                     FDRSPVFFAEGATTPTLILAGGRDKNTPTGQAIEFFGALSEAGAETALAVYPDDGHSL
                     RGYPAYLDSAARIVDWLGRHIRPAS"
     gene            complement(8201..>8906)
                     /locus_tag="VN034_12135"
     CDS             complement(8201..>8906)
                     /locus_tag="VN034_12135"
                     /inference="COORDINATES: protein motif:HMM:NF015120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61401.1"
                     /translation="FALREPRLIPRFAVAPEAAEEARVSIARLLAKPAYRNIVYALVV
                     YFLMAYGALVFTASFMIRVHGLTVAEAGALFGAMSAVGAVVGNLGGGKIGDHLAKRDL
                     AWLARFPGIALILALPLYVGAFAVGNLVLMAAILLVGGILLTGAVPAIFAAMHTVCGS
                     KRRAMAVALAFFFANLIGLGAGPVLAGAISDHFAGIYGPAEGLRYALIIMSLTFLPAG
                     IFMLRAAKHLNADAED"
BASE COUNT         1512 a         3062 c         2921 g         1411 t
ORIGIN      
        1 ctcggcagcg ccctcgatcg ccggcgccca gccgggctgt tccgcgccac ccgattcgag
       61 gccgatcgcc atcgccccct gcgcgccggc gaggtccgat ccatagcccg caacgctcag
      121 caggatggtc tttccgtcgg gcgaccattg gagcgcctcg atgaaaccgt cgaccgccgc
      181 tgccgcgcga tccgtgcggt tttccggatc gaagaggcgc aaatgggtga tatgcgcctg
      241 ctcgcgatcc gacaggaaag cgatggtgcc accgtcgggc gaccatgtcg gcgcgctgtc
      301 cgaccgcgga ccgtgcgtca gcgtttccat ctcgcccgac gccagatcga cgagcacgag
      361 gcgggtcgtc gaaccgccgt cgcgcgacga cagcccttcc agcgcgtcgc atatcgaagc
      421 ggcggcgacg acatgccgcc cgtcggggct ggcggcgagc tgcccgagca gcgccgcctc
      481 gcccgtcccc ggccgcagcc atgcacgggt cagatcctcc gcccgcttga acaacgggtg
      541 attgcggagg tccgcttgcg ctgcgacatc gaaggtcatg cgcccggtcc cgcggcgttc
      601 aggccgatct ggtccatcgc gaatgccagc cattcgccgt tccagcgcgc ggcgtccgcc
      661 gggtcatgcg cgccatggcc ggtatcgcgc cagacgcgga gatggaccgg acggtcgccg
      721 gcattggctt cctccagccg cgccgtgaat ttgcggccgt ggaagggcat gcagcccaga
      781 tccttttcgc cgaacacctg atagaccgcg ggatattcca ccccatcgcg gacattgtga
      841 tagggcgacc agtgccggat actcgcggca tgctcgggat cgttcggatc gccataatcc
      901 tcgaaaaata tcgccctgac cccggcgacg gcgggtgtgt cgggcagcgg ctccatcatg
      961 tcgaagatcg gcacggtcgg cacgacggcg cgccacagat gcggctgttg gacgattgcc
     1021 acaccggcga gcaggccgcc gttgctgccc cccatgaaga tcatcttgtc gggaaccgac
     1081 acgccgtcgg cgatcagcgc ctcggccacc gcctcgagat cgtcgaaggt gttctgcttg
     1141 ttgaacaaac gtccgccgtc gtgccacgcc ttgccatatt cggcgccgcc gcgcagcgac
     1201 gcctgaacga acacgccgcc ggcctcgatg aacgggacgt gcgtcgcggg aaaagacggc
     1261 agctgcgaaa gattgaagcc gccataggca tagacgagcg tcggctgcgc gctgccgaga
     1321 tcgacatcct tccggcggac gacgaaatag ggaatgcgcg tgccgtcgcg gctcgtcgcg
     1381 aaccgccgtt cggcaatcgc atcgccgagc tcatgcttcg cctcgccgat caggcgaagc
     1441 tcgccggttt gcgggtcatg cacgaacagc accgacgaac gggtgaaggt cgtatgcgtg
     1501 atcgcgaagg cgtcgctggg gcggatcgcg cgttcggtca gcatcgactg cgacgatccg
     1561 gggttttcga acgggagcgt atcgaccaac cggccctcca gatcgaacac gcgcacgcgc
     1621 agcgacacgt cgacgaggtc gccgacatag aggcggccgc cgaccacacc cgcccagccg
     1681 atgaagcctt cgccctcggg caccagttcg cgccagcttg ccatatcgcg cgagcttgcc
     1741 gccgggatcg cgaccacgcg tccgcgcggg acaccgttgt tgacgcgcgc gacataggtt
     1801 tcgccgtcga tccagctgcc gtcgcactcg ccttcgaagc cgtcggggag gaacggcgtc
     1861 ggctcgagcg tttcgagatc gagcagcgcc agcgcgacat gctcgtgcgg ctcggtgacc
     1921 gcgacgccgc gccggccgtc gggcgagacc tgaagcgtca ggcccgaatg tttggccgcg
     1981 acgaggcttt cggaaagtag caccgcgggg cgtccggctg cgccgtcggc gacgggaaca
     2041 aagcggagtt cgtgcaggcc ttcggcattg cggccgtcga gccagaatcc gctttcgtcg
     2101 ggaagccagc cggggcgcgc acccgaatag agaatggccg gtgtgtcgag cacatgccgc
     2161 ccggtcgcgg cttcatagac cgaccattgg cccatcatgt cgccatcgac gccccatacc
     2221 accgcaacga agcggccgcg cggcgagggt tccatgaacg cgagcatgac attgcccccg
     2281 ccatgcgctt ccgagaccgc gcgcttcgac acgatcgtcc gccccggacc gttcggttga
     2341 tcgctgacgc gcagcaccag atcgtcgcca tcctcggcat agccgaacca cgacgcgccg
     2401 cgcttgcgcg ggacgaaata gccgctggcg tccgccagtt gcagcagccg gtcatgcacc
     2461 gggccatagt tgggcgacgc ctcggccgtt tcgcgcgcga tgcggtcgcg ctcccattgc
     2521 cagtccagcg cttccggcgt gtcttcgtgc aaatgcgcga agcgatcctc gaacgccacg
     2581 ccgccgatga tcttctggac gggtttggct tgcatcattc tggtctccgg attttcgaaa
     2641 attgtgatgg gcggctcgcg gggctcagcc gcgcgtttcc acgcgcgcct tgatgttttc
     2701 gaggtaagcg acgaggtgca tgtcggcgcc cgccgtcatt tcggcgagcg tggctgcgac
     2761 gccggccttg acgtcgccgg cgggcgcatc gatccgcaat tgggtgtgac agtcgatcgc
     2821 gtagcgcgtt ccctcgcccg cttcctgcgc atgataggtc gcatagacgc gcatgccgcc
     2881 gggcaccgac agatagtcgg ccgctgcgct caagcgccgc gcaccttcgt cgcgttcggt
     2941 gatgtggacg atgcgttcat cgacgacacc gtcctgttcg aaacgcatgc gataaccgcc
     3001 gaacaccgcg gcgggatcgt cgatcggctc gatcgcgccc acctcgcgaa acttcttcgc
     3061 ctcgatataa tcgtcgagga tgacgcgcca cacttcatcg ggcggcgcgg ccatgtccat
     3121 cgcgacatga ttgaccatcg ttaccgggat cggctccgcc atattcttcg cctctctttg
     3181 aggacgcgcc ggtctgcttg cgccggcttg tcccgatcgc cccatggcct ggggtcgggg
     3241 agcagattat ggcggcgggt ggacgagctc cattcgataa cgtacaatag tgtatagtga
     3301 tccgccaaaa aggcggcgcg aggagaggcc atgcatcaca cgggcgatgc cccatcgacc
     3361 gcaatgccgg cgttatcgcc gggcagcggc aaccgcgcga atcggcgggc gctcgccctg
     3421 ccgacgcggg ctcggcgctt tccggtcgcc gccgaccggt tgcagccggt cgtggtcgat
     3481 cgcaagatcc atgacatcgt cgttgaacag gatcgtgcta agcccgacac gggcgtcgag
     3541 acatatgcct cgctcggcga cccgcgtcac ttccgcgtcc aggggtctgc aaacggggca
     3601 tcgggctatt atgaatattg cgcgctggac gaaggcttct tcgcggtcat atccgacata
     3661 atctattcga cgccgctccc cttgtcgctg gcggccgaaa atatggtgcg ggtccgcatc
     3721 gccagtgacg gcgacggcga atatgcggcg gcggacggcg accttctcga cctcaaggga
     3781 ccgagcgcgt cgatcatcat cgaaccgccg ggacaagcgc cggccgaggc cgtgtcggcc
     3841 gggcatcatc tggcggccga aatctatgtt caccgcgatg tgctcgcgcg cctctatgcc
     3901 cgcgacgacc atgaactgcc ggctgtcctg caggccttca tcaccggcca tttgcagcac
     3961 acgatcgccc ggcgcctgcc gctcaatccc cagcttctcc gcgcgatgga agatttgcgc
     4021 ggctgcacgc tcgagggccg cagccggcgg ctggtcatcc ggtcgaaagc gatcgagatc
     4081 ctctgccacg ccatcgaagc gctcgcgcag gacgagaatg gcgacgcccc gcccgaagcg
     4141 tcgaagctcg cggcaaacgg cgtcctgaag gcgcagcggc tgctggctga aaatttcgtc
     4201 tcgccgccat cgctcgacag cctcgcccac gaagtcggga tgagccgcag cgggctttgc
     4261 gccgccttcc ggcagatcgt cgggcaatcg gtgttcgact atgtcggcga cctgaggatg
     4321 cagcaggcac tcgcgatgct gaaccggcaa gacgcaacga tcgccgaggt cgcctatgcg
     4381 gtcggctacg gccatccgtc gagcttttcg gtcgcggtgc agcgccgctt cggtaccacc
     4441 gcgagcgaac tgcgccggcg cggattgccc cccgcctgat cctttgccga acgactgccg
     4501 tcaggcgatc gccgcggcct gcccattggt gtagcgcggc gaaatcgcgg tgagatcgcc
     4561 ggtcgccgga tcgcgccgga ccgcgaccca cagcccctgc gcaaagcgcg cctcggcggg
     4621 cgggatgctc ttgacctcga gcccggtcgc gcccagcagt tcgtccggga actcgccctc
     4681 gacgacgcgc aggatcagct cgcgctggct gccgtcgggc gagatcggca gcaagaggcg
     4741 cggcgcatcg gccgcctcct cgatcgtcat gccgaaatcg atcacgttga gcagcgactg
     4801 cgtcgtctga taatggaggc cgacccccat cgacgaccag gcgatttcgg gctgaccgtc
     4861 cttgataaca agtccgacct cgatcgggtt cggaagctgc gcaccgcgcg gcgtcgcggc
     4921 gaccatcgac tgcatatagg cggcgggatc gccgaccgac acgccgtcga cgacgatcgc
     4981 ggtctggccc cagatcaggc aattgatcga gtggcagagc gccgccatgt tcccgtcggc
     5041 atcgaccgcg acaacgacgt ccgaatgcgt gcccttcggc agaaagcgca ccggcgcctg
     5101 atcgaccagt cgctcccaaa gctcgccggc atgttcgcgg gtcagccgcg acgaccggct
     5161 catgtcgatg ccgggaaaag ccttttgctg ctcttcgggg ctttcatagc gcatgccgcc
     5221 gatcgcggcg cagcatttgg cgaggcgaac gaggctgtcg gacgacgtcg accaatggcc
     5281 gatgtccttg atccccgctg cctcggcaag gttgagcgat tcgatgaggt tgaccgagcc
     5341 ttcgcagggc tcgccgagaa ccgcgatcac atggccttcg cgctcgacgg tcgcgggggt
     5401 cgaccagatc gggcgatagt cggcgagatc cgcgagcgtc atcttgccgc cgtcgcgctg
     5461 caccgcctcg acgcagcgcg ccgcccacgc gcccttgtac atataatcgg cgccctcgct
     5521 cgcgacgcgg cggagcgttt cggcgagcgc gggctggcgg aaatggtcgc cctcggtata
     5581 gggcgtgccg tcggacttgt agaagatcga gcgcgtctcg gggaggcgcg cgagatcctt
     5641 tttgcgcacg tcgatgtagc gcgcgaggcc tgccgaaacc gcgaacccgt cctcggcaag
     5701 ttcgatcgcg gcgtcgaaca ggcgcgcgaa gggcagtttg ccgaagcggc ggtgcgccgc
     5761 ctcgagcccg cgcatgaagc cgccgaccat cgccgtccgt cccgacggct cgccttcgag
     5821 catcatgttg gtcacgccgt cggccgcggg attgagcttg cccgggatcg acatcggatc
     5881 gtcctcggcg actatggtgt tccagcccgc gttgagcgag gtcacatcat cgctcgcggc
     5941 gtcataatgc atcagtgaca atataccgaa gaagctgatc accgcgccgc cgccgagcac
     6001 gatctgcgca agcgcgctgc tcatcaccgc atcgaccgcc gagccgcctt gccggagcgt
     6061 ctcttcaccg acgcgcgcgg caagcccgtt gagcgcggtc gaaacggcgc cgcctttgcc
     6121 gcgcgccggc ggattgccgg gaagcgccgt ctgctggatg ttccaataat gctcgcgttc
     6181 ggcttcgggc caggcggaag ggccgagggt catggggagg tcctttgctg gagtgtcagg
     6241 aagcgggccg gatatggcgg cccagccagt cgacgatgcg cgccgcggaa tcgagatagg
     6301 cgggataacc gcgcagactg tgtccatcgt cggggtagac ggcgagtgcg gtctccgctc
     6361 cggcttccga gagcgcgccg aaaaattcga tcgcctgccc ggtcggcgtg ttcttgtcgc
     6421 ggcccccggc gagaatcagc gtcggcgtcg tcgcgccttc ggcaaagaag accgggctgc
     6481 ggtcgaaata ttgcccgccc ggccgccgcg gcgacccttc gagcagcgcc gcgtcaaacc
     6541 acgggatttg cgacgcataa tgctggctgt accagttcgc gaccggtgaa atcggcacgg
     6601 cagccgcgaa acgatcggtc tgcgtcacca gccacgagct cataaagccg ccatagctgg
     6661 tgcccgacac cgcgacgcgg gtggcatcga cgatccccgc ttggaccaga ctttcgatcc
     6721 ccgtgagata atcgtgcata tcctcaccgc ccatatcctt gtggacgcgg cgagcgaaat
     6781 cctggccgcg tccgcccgat ccgcgcgggt tcggcagcag caaggcatag cccttgccga
     6841 cgagcacggg gccggcgcgc aaggcggccg catagcgatt gcgatgcgcc cagaccggcc
     6901 cgccgtggat atcgacgaag agcggaaggt cttttgcctc gccttcgggc aggacgagcc
     6961 agccgccgat ttcaagcccg tcgcggccct gccagcggtg atagcgcatc gcgccgggaa
     7021 tcgcgggcgg ggaaggcgga gcgaaatcgg cgaacacctc ggcctcgccg ttcgagaggc
     7081 gggcaatcgc gggcgggcgg gcatagcctt cgactaccgc gagcaccgat ccgtcggcgc
     7141 cggggaccgc cgagggatac cagccgccga tcgtcagcgt atccgacgcc caatgctcca
     7201 gccgctcgcc ggtcgcgacg ttgatctcgc ccacgacggt ctccgcatcg cggacgcccg
     7261 cgaagagaag atggtcgggg tcgcgccagc gaagatcggt cacttcgacg ccgccggtat
     7321 ccagcacgcg ttcgccatga gcatcgccca aagtaatcgg gccacagatg attccgcgat
     7381 cgctgcagaa ggcttcgaga atggcccagc tattgccgtc gggcgacccc gccggaacgc
     7441 caatctgctc ggcgcccgtg tagcgcgcac gctcagctcc ggtccccgca tcgaggatgc
     7501 gcaggctcgc ttcgtaccag ctgccctcgc tgtgatgatc gctcgcgatc acggcgaatg
     7561 cgtcgtcgcc gagccatgcg gcttcccaga cattgaccgg cggctgagcc accacccgca
     7621 aagtcatcgc gcccgccgtc cagacataga gccgccgcca gagattctgc gcgcccgacg
     7681 acgccacctc gggaagccac gccggtcccc cggcgtcgct gcgcaacgcg aagccgcctt
     7741 cggcgcccga tacgtcggcg cccgaatccg cgcccaggat cagcagatcg cggccgcccg
     7801 gcgaccaggc gagcgcctcg acccgcatgt cgagcgcgat cgccgcgata cgctcgccgc
     7861 tgtccgacat gatgacgagc cgttcctgtc cgtcgccgtc cgcgacatgg gccgcacggc
     7921 catcgggcgc gctccggtaa aggcggccgc gcgcgatgcg ccggacgttc gcgctcgcaa
     7981 ggtccagtcg gcaaatccag ctcgacgggc cgtccgcaag cgtcccttcg aacgactggg
     8041 ccgagaaatg cacgctttgc ccgtcgggcg cggggtcgag atcggtggcg gtccacagat
     8101 gttcctcgcc ggggtagaac agcgtgcgat acagcgcctc cacctcgcgc atcgcggccg
     8161 agccgcgcat atccctttcg atcatttcaa cctctccgga tcagtcttcg gcatcggcgt
     8221 tcagatgctt cgccgcgcgc agcatgaaga ttccggcggg caggaaagtc agcgacatga
     8281 tgatcagcgc gtagcgcagc ccctcggccg gcccgtatat tcccgcgaaa tggtcgctga
     8341 tcgcgccggc cagcacgggc ccggcgccga gcccgatcag gttcgcgaag aagaaagcca
     8401 gcgcgacggc catcgcgcgc cgcttgctgc cgcaaacggt gtgcatcgcg gcgaaaatgg
     8461 cgggcaccgc ccccgtcagc aatatgccgc cgaccagcag gatcgccgcc atcagcacga
     8521 ggtttccgac ggcgaaggcg ccgacataga gcggcagcgc gaggatcaag gcgatcccgg
     8581 gaaagcgggc gagccacgcc aggtcgcgct tggccaggtg atcgccgatc ttgccgccgc
     8641 cgagattgcc gacgaccgcg ccgacggccg acatcgcacc gaatagcgcg cccgcttcgg
     8701 cgaccgtcag gccgtgaacg cggatcatga acgacgcggt gaagacgagc gcgccataag
     8761 ccatcaggaa atagacgacg agcgcataga cgatgttgcg ataggcgggc ttcgcgagca
     8821 ggcgcgcgat gctcacccgc gcctcctctg ccgcctcggg cgcgaccgcg aagcgcggga
     8881 tcaggcgcgg ctcgcgcagc gcgaag
//
LOCUS       DATMSU010000453         8446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_74168, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000453 DATMSU010000000
VERSION     DATMSU010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74168"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1375)
                     /locus_tag="VN034_12140"
     CDS             complement(218..1375)
                     /locus_tag="VN034_12140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV61402.1"
                     /translation="MFFDFIIAGAGIAGASLAYELSAHGSVCLLEAEASPGIHTTGRS
                     AALFAPSYGGGAIRAITRASRSFFDSPPEAFAAAPLLTARGCLYPAREDQRDALDAMS
                     EGIRRSGGAVEEISAARAREMVPLFRDDYLNAAILDPEAMDIDVDALLQGFLRGAKQR
                     GAILRAQSPLVRAEWAGDIWKVLVPGGIIEAPVLVNAAGAWADDVARACGTDPLGLRP
                     LRRTALLVDPPMDIARWPAVIDVDEKFYFKPEAGKLLLSPADETPDYAGDVQAEEIDI
                     AIGVARVQRAIDLPVRRVSHSWAGLRTFAPDRQPVVGFDSGVPGFFWCAGQGGYGIQT
                     APAMARLAAALVRHVDLPDDLLAERVDPNTISPERFNAAPGMPHAIAVDIS"
     gene            complement(1474..2922)
                     /locus_tag="VN034_12145"
     CDS             complement(1474..2922)
                     /locus_tag="VN034_12145"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61403.1"
                     /translation="MRFFETLLLLSLAATFLLLLIPRFRRTRWPLYASLAGLLLSGVQ
                     IAVEGARWQMVPAHALAALFLLGAFLLGRSATPGSPRSPVRRLATGSAIGLAALGLVI
                     AWALPVVVPMFELPAPTGPYPIGTATYHFVDRSRADWGDKNAPRELMVQVWYPATKPA
                     NPRYDTYVQQAMDFSTLAGGMGLPAFFFDHMKQIPTHAIRSAPMAAGGQRFPLILFSP
                     GAQGFRQHNMFQVEELVSQGYVVAGIDHPRAATHVVLPGKRHVEFDAKLIDIPRFFKD
                     PAFGDRIYNYLGQDASFVLDRMIALDRADPKGILTGRLDTAKAGILGMSLGGLTAAEA
                     CRIDERFRACFIQDVFVPHDVMAQGVAQPTMWFTRDADSMRAEGWPEWEVAVHQDSMR
                     TAFESVRSDGYLVKLPGMFHVNYTDFPSIIATPVARKIGMIGAIDPDHGHRVTNAYTL
                     AFFGRHLKGRAEPLLEGPSKRFPEVGFEARHR"
     gene            complement(3025..3654)
                     /locus_tag="VN034_12150"
     CDS             complement(3025..3654)
                     /locus_tag="VN034_12150"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61404.1"
                     /translation="MPNSDTISNPPKAGRGRPRRFDDRRLEVLRTSAHVFSELGFRQA
                     TLEDIAGALEMTRPALYHYAKSKDALLAECGAIARNQLTDAFARAELEKTGAEQLKSF
                     FHAYGDFVTAEFGRCFALTRWNEMPPAERKDTKRISIQNDDNVGRMIERGIADGTLRE
                     CDTLQVSRAIFASFNGMARWWTKRRDPPVAAIADTILDAFLNGLAPRKG"
     gene            complement(3721..4581)
                     /locus_tag="VN034_12155"
     CDS             complement(3721..4581)
                     /locus_tag="VN034_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWV61405.1"
                     /translation="MSVRLYGMTCGWLTMPFGFFLPGAEGWLAIPVPAYLIVHPKGTA
                     IFDTGLETALQSKDPGEVDATLGAAAGYALPTFEAGEDVGGRLRDFGVDPERIDYVIN
                     SHLHLDHCGGNAMIPNARLVIQKREWEASRTPELIVQNHYTPRHYDLGHDRLEIDGEH
                     DLFGDGSVVLLPTFGHTPGHQSVRVKLADGEVVLAADACYLRETLEKMLLPDPGVVSN
                     ADAMRENYAFLRQIEKQGGLIVFGHDPDQWRHLNSGPVEEITSAAVLDARRTARPNRP
                     EALASPFIGR"
     gene            complement(4626..7448)
                     /locus_tag="VN034_12160"
     CDS             complement(4626..7448)
                     /locus_tag="VN034_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61406.1"
                     /translation="MKKAGIYQPGLIAMRSFGRVAWFACASLAALAAASTAFAQDSGS
                     DAAERSADAETAGDDIVVTASRVERSGFIAPTPTTVVGAEEFGKTGASSIGSLLNRLP
                     QFQGSSTPTTTPLGDGITNGQNRLDLRGLGAKRTLVLVDGKRFVPTNVDGSLDTNLIP
                     AALIDRFEVVTGGASASWGSDAVAGVVNIILKKRFTGVEVSAQAGLSSRGDNVEKKAS
                     LAFGTDFAGGRGHFMAAGEIVRNDGILAQSSRPWGRRGYQLIDNPDYTPENGQARQLI
                     AENVKLAIATRGGLFLFGPPGVGMMQFTPDGQAVPFETGDYFGGPGSIAMIGGDGYNP
                     GADIQLLSPFKRQVAYARLSYDVADEVTAYVEGSYGHSSVRNDRSYAPAILGEVLITP
                     ENPYLPDTIRDAMNLYGIPALPLFRYSTDFGYTRNKGDKDVYRIVGGLEGGFGGGWKW
                     KASYGYGVTDADGYLPNNLIMSRLVEAADVVASPVDGSPVCRSTLTAPGNGCLPINLF
                     GEGAPDPAAVARVVGTSTLLSRLTQHAASASIAGEPFSTWAGPVSIATGVEYRKESVR
                     IDSDLLSQDDAFQVGNAKPLAGSYDVVEGFGEAVVPLLSDQPFAKQLDLSAAIRVADY
                     SSSGRVTTWKLGLSYEINDNLRLRGTRSRDIRAPNLNELFRPSELRYGSVLDPETGGS
                     VTTALLTVGNTSLQPEKADTTSFGVVYQPRWLRGFRLSVDYYDIKLAGAISTLQPQDI
                     VNRCAAGNDAFCDFVTRNGAGNIQSITLSQINLGKVRTEGVDVEALYAMPVGEGDLTL
                     RLLSTYVAHLTTDDGVTAIDRAGDVGADAMGVPHWRATGSLSYDRGPLSLYLAGRYVG
                     GGKYDHSASIANNDIDGVFYLDGSVQFTIKEEGRRQIQLTLAGNNLLDKDPPIAPSAF
                     FIPIATNAALYDVIGRTFTAGVRVTF"
     gene            complement(7625..7849)
                     /locus_tag="VN034_12165"
     CDS             complement(7625..7849)
                     /locus_tag="VN034_12165"
                     /inference="COORDINATES: protein motif:HMM:NF015214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HWV61407.1"
                     /translation="METECRQRILRLIENMTLGRNAVGYLTESMHGAGSPQAQRIQIA
                     RGMGVESKKELARKLAGIEEDRAAAAQFRP"
     gene            complement(7813..8094)
                     /locus_tag="VN034_12170"
     CDS             complement(7813..8094)
                     /locus_tag="VN034_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61408.1"
                     /translation="MHAKKEPPRDESADERWARFTPIRTGEDYVESLRSRGVAVHMFG
                     ERIAEPVDHPVIRPSTTGFSAMRRLGRCCANIFRAGRAWRPNAASVSSD"
BASE COUNT         1545 a         2764 c         2646 g         1491 t
ORIGIN      
        1 caacgccgtg gacctgaaac accgacttcg cgatatccaa cccgactgtt gcaatcttct
       61 ccatcgtagc tctccttcca gctcatcccg cggatcatac cgcgggaggt tggagagccg
      121 tccacgtcat cagttgcgga cggtccgctt ttgagagcga attgcattcc acgttagacg
      181 accgctaacg cgggcacatc catcccatgc cttctgttca agaaatatcg accgcaattg
      241 catgaggcat gccgggcgcc gcgttgaacc gttcaggtga tatcgtgttc gggtcgaccc
      301 tttcggcaag caagtcgtcg gggaggtcca catgccgcac tagcgccgcg gcgaggcggg
      361 ccatcgccgg tgccgtctga attccgtagc cgccctgccc cgcgcaccag aaaaaaccgg
      421 gaaccccgct gtcaaatcca acgaccggct gtcgatcggg cgcgaatgtg cgcaagcccg
      481 cccagctgtg cgacacgcgc cttaccggca ggtcgatcgc gcgctgaacc cgggcgacac
      541 cgatcgcgat gtcgatttct tccgcctgaa cgtctccggc atagtccgga gtctcgtcgg
      601 ccggcgacag aaggagcttg cctgcttcgg gcttgaaata gaatttttca tcgacatcga
      661 tgacggctgg ccaacgcgcg atatccatcg gcggatcgac gagaagagcg gtccgcctga
      721 gcggacgcag tccaagcggg tcggtgccgc atgcacgcgc gacgtcgtcg gcccacgccc
      781 ccgcggcatt gacgagcacc ggcgcttcga tgatgccgcc ggggacgagg accttccaga
      841 tatcgccggc ccattcggct cgcaccagcg gactttgcgc cctgaggatg gcgcctcgct
      901 gctttgcgcc gcgcaaaaag ccctgaagga gcgcatcgac atcgatatcc atcgcctcgg
      961 gatcgaggat ggcggcattg agataatcgt cgcggaagag cggcaccatt tcgcgcgccc
     1021 ttgccgcgct gatttcttcg acggcaccgc ccgaacgccg tatgccctcg ctcatggcat
     1081 caagcgcatc gcgctgatcc tcgcgtgccg gatagagaca tccgcgcgcg gtcaggagcg
     1141 gcgctgccgc aaaagcttcc ggcgggctat cgaagaagct acggctcgcc cgcgtgatcg
     1201 cacgaatggc gccgccacca tagcttgggg cgaagagcgc cgccgaacgc cccgtcgtgt
     1261 gaataccggg gctcgcttcg gcttcgagca ggcaaacgct gccgtgcgcc gacagttcat
     1321 aggcgagcga cgcgcccgcg atgccggcgc cggcaatgat gaaatcgaaa aacatcgagc
     1381 ggaactccac cttggcgagc gcaaaccgtc gctcgccggg gttcggcgac gatcatcgct
     1441 cggatcggtt aatgggcggc gcgaaaggcg tgatcagcga tgccgggcct cgaaccccac
     1501 ttctgggaac cgcttcgacg gaccctccaa cagcggttcg gccctcccct tcagatgacg
     1561 tccgaagaag gcgagcgtgt aggcgttggt aacgcggtgc ccatggtcgg ggtcgatcgc
     1621 cccgatcatg ccgatcttcc gcgcgaccgg ggtcgcgatg atcgacggga agtcggtgta
     1681 gttcacatgg aacatgccgg gcaacttcac cagatagccg tcggagcgga cgctctcgaa
     1741 cgccgtacgc atgctatcct gatggacggc cacctcccat tcgggccagc cttcggcgcg
     1801 catgctgtcg gcatcgcgcg tgaaccacat cgtcggctgc gccactcctt gtgccatcac
     1861 atcgtgcgga acgaagacgt cctgaatgaa gcaggcgcgg aagcgctcgt cgatgcggca
     1921 cgcttctgcc gcggtcagcc cgccaagcga catcccgagg atgcccgcct tcgccgtgtc
     1981 cagccggccc gttagaatgc ccttgggatc ggcgcggtcg agcgcgatca tccggtcgag
     2041 cacgaacgac gcgtcctgcc ccagatagtt gtagatgcgg tcgccgaacg cgggatcctt
     2101 gaagaaacgc gggatgtcga tcagcttggc gtcgaattcg acatgccgct tgccgggaag
     2161 gacgacgtgg gtagcggcgc gcggatggtc gatgccggcg acgacatagc cctgtgacac
     2221 cagctcttcg acctggaaca tattgtgctg ccgaaagccc tgcgcacccg gagagaaaag
     2281 gatcagcggg aaacgctggc cgcccgccgc catcggcgcc gacctgatcg catgcgtcgg
     2341 aatctgcttc atatgatcga agaagaaggc gggaagcccc atgccgcctg cgagcgtcga
     2401 aaagtccatg gcctgctgga cataggtgtc gtagcgcgga ttggcgggct ttgtggcggg
     2461 ataccagacc tgaaccatca attcgcgcgg cgcgtttttg tctccccaat cggcccgcga
     2521 ccgatcgacg aaatgatagg ttgccgtgcc gatcggataa gggccggtcg gcgcgggcag
     2581 ctcgaacatc ggaacgacga cgggcaaggc ccatgcgatg accagcccca aggccgcaag
     2641 gccgatcgcc gatcccgtcg ccagccgccg gaccggcgag cgaggactcc cgggcgtggc
     2701 ggacctgccg agcaggaacg caccgagcag gaacaaggcg gccagagcat gggccggaac
     2761 catctgccag cgcgcgcctt cgacggcgat ctgcacgccc gacagcaaaa gtccggcgag
     2821 gctcgcatag agcggccatc gcgtccggcg gaagcgggga atgagcagaa gcagaaaggt
     2881 cgctgcaagg ctgagcagca gaagcgtctc gaaaaatctc atcctcattc cccaattgcc
     2941 cggcgactca cgcgccatgg cgaggggata atttttactt actgtaaagt caatatattc
     3001 aacacgcgac gacggccgcg gcgatcagcc ttttcgtggc gcgagaccgt tcagaaaggc
     3061 gtcgaggatc gtgtcggcga tcgcggccac cggcgggtca cgccgcttgg tccaccagcg
     3121 cgccatgccg ttgaagctcg cgaagatcgc gcggctgacc tgcagcgtat cgcattcgcg
     3181 cagcgtgccg tcggcgatgc cgcgctcgat catccgcccg acattgtcat cgttctggat
     3241 gctgatgcgc ttggtgtcct tgcgctcggc gggcggcatt tcgttccagc gggtgagcgc
     3301 gaagcagcgt ccgaattcgg ccgtcacaaa gtcgccatag gcgtgaaaga agctcttcag
     3361 ctgttcggcg ccggtctttt cgagttcggc acgcgcgaag gcgtcggtca actggttgcg
     3421 cgcgatcgca ccgcattcgg cgagcagcgc atccttgctt ttcgcataat gatagagcgc
     3481 gggccgcgtc atctctagcg cgccggcgat atcctcgagc gtggcctgcc ggaaaccgag
     3541 ttcgctgaag acatgcgccg acgtgcgaag cacctcgagc cgccggtcgt cgaaacgccg
     3601 tgggcgcccg cggccggcct tgggcggatt ggatatggtg tcgctgttcg gcatgtgccc
     3661 ctcatctgac cctgtgtcga ctgatcagta agtcaatttt gcggggatcg ccatgcccca
     3721 tcatcgcccg atgaaaggcg acgccagcgc ttcgggccga ttgggccgcg ccgtgcgccg
     3781 ggcatcgagg acggccgcac tggtgatttc ctcgaccggc cctgaattga gatggcgcca
     3841 ttggtcggga tcgtgaccga agacgatgag gccgccctgc ttttcgattt gccgcagaaa
     3901 ggcgtaattc tcgcgcatcg catcggcatt gctcaccacg cccggatcgg ggagcagcat
     3961 tttttcgagc gtttcgcgca gataacaggc atcggcggcg agcacgactt caccatccgc
     4021 aagcttcacg cgcaccgact ggtgccccgg cgtatggccg aaggtcggca acagcacgac
     4081 cgagccgtcg ccgaacaggt catgttcgcc gtcgatttcc agccggtcgt gcccgagatc
     4141 atagtgacgc ggcgtataat ggttttgtac gatcagctcg ggcgtccggc tcgcttccca
     4201 ttcgcgcttc tggatcacca gccgtgcatt gggaatcatc gcgttccccc cgcaatgatc
     4261 gagatgaagg tgcgagttga tgacatagtc gatccgttcc gggtcgacgc cgaaatcgcg
     4321 aagccggccg ccgacatcct cgcccgcttc gaacgtcggc aaggcgtaac ccgctgccgc
     4381 gcccaacgtc gcgtcgactt cgcccggatc cttagattgc agcgccgtct ccagccccgt
     4441 gtcgaagatc gccgtgccct tgggatggac gatcagatag gccggaaccg gaatggcgag
     4501 ccagccttcc gcgcccggca ggaagaagcc gaacggcatg gtgagccagc cgcaggtcat
     4561 tccgtacaat cgaacgctca tcttttctct ccttccatct cagctctagc agacccgccc
     4621 tggcgtcaga aggtaaccct cacgcccgcc gtgaacgtcc gcccgatcac gtcatagagc
     4681 gcggcgttgg tcgcgatcgg gatgaagaag gccgagggcg cgatcggcgg atccttgtcg
     4741 agcagattgt tgccggccag cgtcagctgg atttgccgcc gcccctcctc cttgatcgtg
     4801 aactgcaccg atccgtcgag atagaaaacc ccgtcgatgt cgttgttcgc gatgctggcg
     4861 ctatgatcat atttgccgcc gccgacatag cggcccgcaa gatagagcga gaggggcccg
     4921 cgatcgtagg acaggctgcc cgtcgcgcgc cagtggggaa cacccatcgc atcggcgccg
     4981 acgtcgcccg cgcggtcgat cgcggtcacg ccgtcatcgg tggtaaggtg cgcgacatag
     5041 gtcgacagca gccgcaatgt cagatcgcct tcgccgaccg gcatcgcata cagtgcctcg
     5101 acatcgacac cttcggtccg caccttgccc agattgattt gcgacagggt gatgctctgg
     5161 atgttgccag caccgttccg ggtcacgaaa tcgcagaagg catcgttgcc cgccgcgcag
     5221 cgattgacga tatcctgcgg ctgcagcgtc gagatcgcgc cggccagttt gatgtcataa
     5281 taatccaccg acagccggaa cccgcgcagc caccgcggct gatagacgac gccgaacgag
     5341 gtcgtgtcgg ccttttcggg ttgcaggctc gtattcccga cggtcagcag cgcggtggtg
     5401 acgctgcccc cggtctcggg atcgagcacc gagccgtaac gcagttccga cgggcggaac
     5461 aattcgttga ggttgggcgc gcgaatatcg cgcgagcgcg tgccgcgcag gcggagattg
     5521 tcgttgatct cgtatgacag gcccagcttc cacgtcgtga cgcggccgct gctgctataa
     5581 tccgcaacgc ggatcgccgc gctgagatcg agctgcttgg cgaacggctg atcggacaac
     5641 agcggcacga ccgcctcgcc gaaaccttcg accacatcat aggagcccgc aagcggcttt
     5701 gcattgccga cctgaaaggc atcgtcctgc gacagcagat cggagtcgat ccggacgctt
     5761 tccttgcgat actcgacccc ggtcgcgatc gagaccggcc ccgcccaggt cgagaagggc
     5821 tcgcccgcga tactggcgct cgccgcatgc tgggtgaggc gcgagagcaa tgtcgatgtt
     5881 ccgacgacgc gcgccacggc cgcgggatcg ggcgcccctt cgccgaacag gttgatcggc
     5941 aggcaaccgt tgccgggagc ggtcagcgtc gagcggcaga ccggcgatcc gtcgaccggc
     6001 gaggcgacga catcggccgc ctcgaccagc cgcgacatga tcaggttgtt cggcagatag
     6061 ccgtcggcgt cggtcacgcc atatccgtac gacgccttcc acttccagcc gccgccgaac
     6121 ccgccctcga gcccgccgac gatgcgatag acatccttgt cgcccttgtt gcgggtatag
     6181 ccgaaatcgg tgctgtagcg gaacagcggc agcgccggga tgccatagag gttcatcgcg
     6241 tcgcggatcg tatccggaag atagggattc tcgggcgtga tcagcacttc gccgaggatt
     6301 gccggcgcat aggaccggtc gttgcggacg ctactgtggc cgtaactacc ctcgacatag
     6361 gcggtcactt catcggccac gtcgtagctc agccgcgcgt aagcaacctg ccgtttgaag
     6421 ggcgagagaa gctggatatc ggcgccgggg ttgtatccgt cgccgccgat catcgcgatg
     6481 ctgccggggc cgccgaaata gtcgccggtc tcgaacggca ccgcctgccc atcgggagtg
     6541 aactgcatca taccgacgcc cggcgggccg aacaggaaca agccgcccct ggtcgcgatc
     6601 gcaagtttca cattctcggc gatcaactgc cgcgcctggc cgttctccgg cgtataatcc
     6661 gggttgtcga tcagctggta accgcggcgg ccccacggac gcgaggactg tgcaaggatg
     6721 ccgtcgttcc ggacgatctc gcccgcagcc atgaaatgcc ccctgccccc ggcgaagtcg
     6781 gtaccgaacg cgagcgaggc ctttttctcg acattgtcgc cgcgcgagga gaggccggcc
     6841 tgcgccgaaa cctcgacccc ggtgaagcgc ttcttgagga tgatgttcac caccccggcg
     6901 accgcgtcgg agccccacga ggccgaagcg ccaccggtca ccacctcgaa ccggtcgatc
     6961 agcgctgcgg ggatcaggtt ggtgtcgagc gagccgtcga cattggtcgg cacgaagcgc
     7021 tttccatcga ccagcacgag cgtgcgcttc gcgcccagcc cgcgaagatc gaggcgattc
     7081 tggccgttgg tgatgccgtc gcccagcggc gtcgtcgtcg gggtcgacga cccctggaac
     7141 tgcggcagcc ggttgaggag cgatccgatg ctcgacgcgc cggtcttgcc gaactcttcg
     7201 gcgccgacca ccgtcgtcgg cgtcggcgcg ataaagcccg accgctcgac gcgcgaggcg
     7261 gtcacgacga tatcgtcgcc cgcggtctcg gcatcggctg accgttcggc ggcatcggac
     7321 ccgctatctt gcgcaaacgc ggtcgatgcc gcggcgagcg ccgccaacga ggcgcacgcg
     7381 aaccaggcca cacggccgaa ggatcgcatg gcgatcagac ccggctgata aattcccgcc
     7441 tttttcatca gattttccct cttcctcagg atcggcaaac gcccggccgc aacggcggcc
     7501 cggaacgatg atcgtctcct gcgctctccc ggatgtctgg ccgcctgtag ccgcattgcg
     7561 gttcggcggg ttccccggct gctgcggggc atgcaattat tctgacttac tgtaaagtaa
     7621 aatattatgg ccggaactgc gccgctgcgg cgcggtcctc ctcgattccc gccagcttcc
     7681 gggcgagttc cttcttgctt tcgacgccca tgccccgcgc gatctgaatc cgctgcgctt
     7741 gcggcgaccc ggcgccgtgc atcgattcgg tgagatatcc gacggcattg cggccgagcg
     7801 tcatattctc gatcagtcgg aggatacgct ggcggcattc ggtctccacg cccgccctgc
     7861 cctgaagata tttgcgcagc atcggcccag tcgtcgcatc gccgaaaatc ctgttgtcga
     7921 gggccggatg accggatggt cgacgggctc ggcgatgcgc tcgccgaaca tatggaccgc
     7981 gacgccgcgc gaccgcaggc tctccacata atcttcgccc gtccggatcg gcgtaaagcg
     8041 cgcccagcgt tcgtcggcgc tctcgtcgcg cggcggttcc tttttcgcgt gcatcctgca
     8101 tctccaccaa atcggtgccg gctatgcctc gccgtcgcat acgtcaatat atttttgact
     8161 tactagtcag tcatattaaa accggcgagc gacgcgtgac gcgcaatttt cgacttgcgc
     8221 tcgctccaca tttatgtgac tgcccagtaa gtaaaataac aatgcggatc gcccgcgacc
     8281 tagagggatg accaatggcc aacataccgc tcggcgacct gatccggcac catgcgcggc
     8341 gaaagcccgc tggcacctct gcgctgatcc atcccgatgc cgaagtcagc tgggcggagc
     8401 tcgacgcgct ggcgagcgcc aaggccgcgt tttaccgcgc gcgcgg
//
LOCUS       DATMSU010000454         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_178311,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000454 DATMSU010000000
VERSION     DATMSU010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178311"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..611)
                     /locus_tag="VN034_12175"
     CDS             complement(90..611)
                     /locus_tag="VN034_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61409.1"
                     /translation="MRLVITAIAPIAAGLWISNPAPAQAPAPFDGSWMSCETYQSAEI
                     CEYKMLVQRGDRLCGVQRYFATNAYYEQRFVGTVKANVAHIEKICGDPGSETDTYCSG
                     RAPAGAAKVGWQAIDRKLGLCGGRLVDGGRDFHSCTGASRSLGMPRANELGEQGPGPE
                     DRAWLASCAGGAE"
     gene            850..2070
                     /gene="hemA"
                     /locus_tag="VN034_12180"
     CDS             850..2070
                     /gene="hemA"
                     /locus_tag="VN034_12180"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HWV61410.1"
                     /translation="MNYNDIFARAIDRLHEEGRYRVFIDILRNKGAFPNARCFAGHNG
                     PKPITVWCSNDYLAMGQHPKVVAAMEEALHDVGAGSGGTRNIGGNTHYHIDLEAELAD
                     LHGKDGALLFTSGYISNEATLGTLGKLLPNCIIYSDELNHASMIAGIRNSGCEKRVWR
                     HNDLEHLEELLAADDPEAAKLIAFESVYSMDGDVAPIHAICDLAEKYNALTYCDEVHA
                     VGMYGPRGGGITDRDEAAHRVTIIEGTLGKAFGVMGGYIAAEKNIIDVIRSYAPGFIF
                     TTSLSPVLVAGVLASVRHLKSSSVEREAQQEAAAYLKQSFRDAGLPVMDSTTHIVPLM
                     VGDPVRAKKISDILLAEYGVYVQPINFPTVPRGTERLRFTPGPAHDEAMMRELTSALV
                     EIWDRLELQLLKAA"
     gene            2067..2756
                     /locus_tag="VN034_12185"
     CDS             2067..2756
                     /locus_tag="VN034_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWV61411.1"
                     /translation="MRTASGYDDVVVGRRSIRGFLDKPVPRELIAEILEVAMRAPSSF
                     NNQCWNFSVVTGAPLAAIRRGNTDGILAGKPDSREFRRFDGIADDHRARQIEIAKQLF
                     GAMGIAREDKDARQDWVLRGFRQFDAPVSIVVTYDRVLLGSDIAPFDCGAVTNALVNA
                     AWSRGLGCVINSQGIMQSPVVREHARIPDDQVIMICVAMGWPDGDFPANAVVSNRKSV
                     DDAVRFVGFDD"
     gene            2832..>3386
                     /locus_tag="VN034_12190"
     CDS             2832..>3386
                     /locus_tag="VN034_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWV61412.1"
                     /translation="MPAQRYSYTAITLHWLIALLLALQIALGWALEGNNSPELFARYQ
                     LHKSIGITILLLSIARLAVRLFTPRPPASEGPAWTRALAGTVHWLFYLVMILGPLTGW
                     LLVSTARVQVPTLLYGILPWPHLPVGRSWHEPAESIHGAMAWIAIGLFLLHIAGALRH
                     QWLLGKPELQRMIPFARGKAVGAAI"
BASE COUNT          580 a         1153 c         1055 g          598 t
ORIGIN      
        1 tgccgtcttc acccgcagcg acgaccgacc gccccccgcg ctcgccgcgc tcgcgcccta
       61 tggcatcgga tcggtcgaaa cgatctagtt cactcggcac cgccggcgca cgacgccagc
      121 caggcgcgat cctcgggccc ggggccctgc tcgcccagtt cgtttgcgcg cggcatcccc
      181 aagctccgac ttgcgccagt gcaggagtgg aaatcgcgcc caccgtcgac cagccgcccg
      241 ccgcacaggc ccagcttccg gtcgatcgcc tgccagccca ccttcgccgc gcccgcaggc
      301 gcgcgtccgc tgcaataggt gtcggtctcc gaccccgggt cgccgcagat tttctcgata
      361 tgcgcgacat tggctttcac cgtgccgaca aagcgctgtt cataataggc gttggtcgcg
      421 aaatagcgct ggacgccgca tagccggtcg ccgcgctgga cgagcatttt atactcgcag
      481 atttccgcgc tctgataggt ttcgcagctc atccagcttc catcgaaagg tgccggtgcc
      541 tgcgcgggtg ccgggttcga tatccaaagt cccgcggcga tcggcgcgat ggcggtgatg
      601 acaagacgca tagcggactc tttcctttcc gtttccattc gaatcgcgac tagccgcccc
      661 ggatcgactg cggggtgcga tcgatcacag caaccggcaa ggtcacgacg cggtgggaca
      721 atttgcccca gttctgcgaa tcgttcgcac tggccttcga cgcttttcgg cgttattggc
      781 ccgcatgtta atagccgccg ggggagcggc gcccagagct tgactggcac ctgactgcgg
      841 ggctgaaacg tgaattacaa cgacatcttc gcacgcgcga tcgaccggct gcacgaagag
      901 ggacgctacc gcgtcttcat cgatatcctg cgcaacaagg gcgctttccc gaacgcgcgc
      961 tgcttcgccg gccataacgg gccgaagccg atcaccgtct ggtgctcgaa cgactatctc
     1021 gcgatgggcc agcatcccaa ggtcgtcgcc gcgatggaag aggcgctgca cgacgtcggc
     1081 gcgggctcgg gcggcacgcg caacatcggc ggcaacacgc attatcacat cgaccttgag
     1141 gccgagcttg ccgacctgca cggcaaggat ggcgcgctgc tcttcacctc gggctatatc
     1201 tcgaacgagg cgacgctggg cacgctcggc aagctgctcc cgaactgcat catctattcg
     1261 gatgaactca accacgcatc gatgatcgcg ggcatccgca attcgggctg cgaaaagcgc
     1321 gtgtggcggc acaacgacct tgagcatctc gaggagctgc tcgccgccga cgatcccgag
     1381 gcggcgaagc tgatcgcgtt cgaaagcgtc tattcgatgg acggcgatgt cgccccgatc
     1441 cacgcgatct gcgaccttgc cgagaaatat aacgcgctca cctattgcga cgaagtccat
     1501 gccgtcggca tgtacggccc gcgcggcggc ggcatcaccg accgcgacga agccgcgcac
     1561 cgcgtgacga tcatcgaagg cacgctcggc aaggcattcg gcgtgatggg cggctatatc
     1621 gctgccgaga agaatatcat cgatgtgatc cgctcctatg cgccgggctt catcttcacg
     1681 accagcctgt cgccggtgct cgtcgcgggc gtgctcgcga gcgtgcggca cctcaaatcg
     1741 tcgagcgtcg aacgcgaggc gcagcaggag gccgccgcct atctcaagca gagcttccgc
     1801 gatgcgggtc tgccggtgat ggattcgacg acgcacatcg ttccgctgat ggtcggcgac
     1861 ccggtgcgcg cgaagaagat cagcgatatc ctgctcgccg aatatggcgt ctatgtgcag
     1921 ccgatcaatt tcccgaccgt cccgcgcggc accgagcgcc tccgcttcac gccgggcccc
     1981 gcgcatgacg aagcgatgat gcgcgagttg acgagcgcgc tggtcgagat ctgggaccgg
     2041 ctcgaactgc agcttctgaa ggcggcgtga ggaccgcgtc cggttatgac gacgtcgtcg
     2101 tcggacgccg ctcgatccgc ggcttcctcg ataaacccgt tcccagggag ctgatcgccg
     2161 agatactcga agtcgcgatg cgcgcgcctt cgtcgttcaa caaccagtgc tggaatttct
     2221 cggtcgtcac cggcgcgccg ctcgctgcga tccggcgggg aaacaccgac ggcatcctcg
     2281 ccggcaagcc cgacagccgc gaattccgcc gcttcgacgg catcgctgac gaccatcgcg
     2341 cgcgccagat cgagatcgcc aagcaactct tcggcgcgat gggcatcgcg cgcgaggaca
     2401 aggacgcgcg tcaggattgg gtgctgcgcg gctttcgcca gttcgacgcg ccggtcagca
     2461 tcgtcgtcac ctatgaccgc gtgctgctcg gcagcgacat cgcgccgttc gactgtggcg
     2521 ccgtcaccaa cgcgctggtc aacgccgcct ggtcgcgcgg gctcggctgt gtgatcaaca
     2581 gccagggcat catgcagagc cccgtggtgc gcgaacatgc gcgcatcccc gacgatcagg
     2641 tcatcatgat ctgcgtcgcg atgggctggc ccgacggcga ctttccggcg aatgcggtcg
     2701 tgtcgaaccg caagagcgtc gatgatgcgg tacggttcgt cgggttcgac gattgattcc
     2761 cgggcgacag ccgattggca aggcgatagg ctaaggcgcg aatccgacgc cgacgatcac
     2821 gaaagattgc catgccagca cagcgttata gctataccgc catcacgctg cactggctca
     2881 tcgcgctgct gctggccctc cagatcgcgc tgggctgggc gctcgaaggc aacaacagcc
     2941 ccgagctctt cgcgcgttat cagctgcaca aatcgatcgg catcacgatc ctgctgctca
     3001 gcatcgcgcg gctcgcggtt cgcctgttca cgccgcgccc gcccgcatcc gaaggcccgg
     3061 catggacgcg cgcgcttgcc ggcacggtgc actggctctt ctacctggtg atgatcctcg
     3121 gcccgctcac cggctggctg ctcgtttcga ccgcgcgcgt gcaggtgccg actttgctct
     3181 acggcatcct cccatggccg cacctgcccg tcgggcgcag ttggcacgaa cccgccgaga
     3241 gcatccacgg tgcgatggcg tggatcgcga tcgggctgtt cctgctgcat atcgcgggcg
     3301 cgcttcgcca tcagtggctg ctcggcaagc ccgagcttca gcggatgatc cccttcgcgc
     3361 gcggcaaggc cgtcggcgcc gcgatc
//
LOCUS       DATMSU010000455         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_83138, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000455 DATMSU010000000
VERSION     DATMSU010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83138"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VN034_12195"
     CDS             complement(<1..1469)
                     /locus_tag="VN034_12195"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWV61413.1"
                     /translation="MGPPIQISQNPDIRYLGRILGDVIRAYGGDKLFRQTEYIRSSSV
                     DRHRGIAGAEAIDPGLDALSLDDTIAFVRGFMLFSMLANLAEDRQGVAAEPEATVAAA
                     IEKLKEDGIDGEAVAALLGASLVAPVLTAHPTEVRRKSVLDHKNRVAELMRLRDAGLD
                     ETPEGDVIEDAIRRQVVLLWQTRPLRTQKLFVADEIDNALTYLRDVFLPVVPKLYARW
                     EKELGTRPASFLRVGSWIGGDRDGNPFVTAETLRQATARNAAAVLGHYIDQVHALGAE
                     LSVSSSLAPVPEAVEALAEASGDAAPSRGDEPYRRALSGIYARLSATYLAITGKAPPR
                     PAALKGEAYATPADFRRDLVTIANGLSASGKGQLAGIGALGRLIRAVEVFGFHLATLD
                     MRQNSAVHERVLAELLSVSGVCADYLALDEKARVALLTAELASDRPLAAPWHQWSDET
                     AGELAIVHAAADVRARLGNDAICQWIISMAQELSDLLEV"
     gene            complement(1533..1958)
                     /locus_tag="VN034_12200"
     CDS             complement(1533..1958)
                     /locus_tag="VN034_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HWV61414.1"
                     /translation="MVRISRMLALSAIAAPLLAACATIPPAEEPVSVTGSITYRERIA
                     LPPTAQVEITLADVSLADAPSTTIAQQSFTADGRQVPFAFTLTVDQRQLDPRHSYAVS
                     ARITDASGRLMFITDTRNSVAFDGRRTINMGMLTLIKTN"
     gene            complement(2042..2608)
                     /locus_tag="VN034_12205"
     CDS             complement(2042..2608)
                     /locus_tag="VN034_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWV61415.1"
                     /translation="MFHPDLARHPDSCPALVLNADYTPLSYYPLSLWPWQTAVKAVFL
                     DRVTIVENYEREIHSPTRTMPIPSVIALRQYVKPSEHPAFTRFNLFLRDRFACQYCGS
                     GKDLTFDHVVPRRLGGRTTWENVATACAPCNLKKGGRTPQQAHMPLYRQPWRPTSWQL
                     QDNGRAFPPNYLHTSWIDWLYWDVELEG"
     gene            2780..>2993
                     /locus_tag="VN034_12210"
     CDS             2780..>2993
                     /locus_tag="VN034_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWV61416.1"
                     /translation="MLTRFAPSPTGELHLGHAYSAVLAHGAARAADGRFRIRIDDIDG
                     SRSREAFVGASLADLAWLGIDWDGDPV"
BASE COUNT          492 a          968 c         1016 g          517 t
ORIGIN      
        1 tggacttcga gcaggtcgga cagctcctgc gccatgctga tgatccactg gcagatcgcg
       61 tcattgccca gccgcgcgcg gacatcggcg gcggcgtgaa cgatcgcgag ttcacccgcg
      121 gtctcgtcgc tccactggtg ccagggcgcg gccagcggcc ggtcgctcgc cagctcggcg
      181 gtgagcagcg cgacgcgcgc cttttcgtcc agcgccaaat agtctgcgca tacacctgat
      241 accgaaagca gctcggcgag cacgcgttcg tgcaccgcgc tgttctgccg catgtcgagc
      301 gtggcgaggt ggaagccgaa gacctcgacc gcgcggatca accgcccgag cgcgccgatg
      361 cccgcaagct ggcccttgcc gctcgccgac aggccgtttg cgatggtgac gaggtcgcgg
      421 cgaaaatcgg cgggggtggc ataggcctcg cccttcagcg cggctgggcg cggcggtgct
      481 ttgccggtga tcgccagata ggtggccgac agccgcgcat aaatgcccga caatgcgcgg
      541 cgatagggtt cgtcgccgcg gctcggcgca gcgtcgccgc tcgcttccgc cagcgcttcg
      601 accgcctcgg gcaccggcgc gaggctcgac gacaccgaca gctcggcgcc gagcgcatgg
      661 acctggtcga tataatggcc gagcaccgcc gcggcgttgc gcgctgtcgc ctgtcgcagc
      721 gtctcggcgg tgacgaaggg gttgccgtcg cggtcgccgc cgatccagct cccgacgcgc
      781 aggaaactcg cggggcgcgt ccccagctcc ttttcccagc gcgcataaag cttgggcacc
      841 acgggcagga agacgtcgcg caaataggtg agcgcattgt cgatctcgtc ggcgacgaac
      901 aatttctgcg tgcgcagcgg ccgcgtctgc cacagcagta cgacctgccg ccggatcgcg
      961 tcctcgatca cgtcgccctc gggcgtctcg tcgagcccgg cgtcgcgcag ccgcatcagt
     1021 tcggcgacgc ggttcttgtg gtcgagcacc gacttgcgcc gcacttcggt cggatgcgcg
     1081 gtgagcaccg gcgcgacgag cgacgcgccg agcagcgcgg cgaccgcctc gccgtcgatc
     1141 ccgtcctctt tcaatttctc gatcgccgcc gcgacggtcg cctcgggttc ggcggcaacg
     1201 ccctgccggt cctcggcaag gttggcgagc atcgaaaaca gcatgaagcc gcgcacgaag
     1261 gcgatcgtgt cgtcgaggct gagcgcatcg agacccggat cgatcgcctc ggcccccgcg
     1321 atgccgcgat gccggtcgac gctcgacgag cggatatatt cggtctggcg gaacaatttg
     1381 tcgccgccat aggcgcggat gacgtcgccg aggatccgcc ccaaatagcg gatgtcggga
     1441 ttttgcgaga tctggatagg cgggcccatg cgccgcttgc tgcacctgcg aaggtgcagg
     1501 gtcaagcggc gcatagctat gcgcgcgtca tatcaattcg tcttgatgag ggtcagcatg
     1561 cccatgttga tcgtccggcg gccgtcgaac gcaacagagt tgcgcgtgtc ggtgatgaac
     1621 atcagtcttc ccgacgcgtc ggtgatgcgc gccgacacgg catagctgtg acgcgggtcg
     1681 agctggcgct gatcgacggt cagcgtgaag gcgaagggca cctggcggcc atcggcggtg
     1741 aagctttgct gcgcgatcgt tgtcgatggc gcatcggcca ggctgacgtc ggcaagggtg
     1801 atttcgactt gcgccgtcgg cggcagcgcg atgcgctcgc gataggtgat gctgccggtg
     1861 accgacaccg gctcttcggc gggcgggatc gtcgcgcagg cggcaagcag cggggcggcg
     1921 atcgcactga gggcgagcat gcggctgatg cgaaccatga tgtttctcct tttttcgatg
     1981 gtcggcggcc gcctaatccc atcgaggtga acccatgctg tcgtgcccgc ccatgcgccg
     2041 ttcagccttc gagttcaacg tcccaataga gccagtcgat ccagctcgtg tgcagataat
     2101 tgggcgggaa cgcgcggcca ttgtcctgca actgccagct cgtcgggcgc cacggctggc
     2161 gatagagcgg catatgcgcc tgctgcggcg tccggccgcc cttttttaga ttgcacggcg
     2221 cgcaggcggt cgcgacattc tcccacgtcg tgcgcccgcc gagccggcgc ggcacgacat
     2281 gatcgaaggt cagatccttg cccgacccgc aatattggca cgcgaagcgg tcgcggagga
     2341 acaggttgaa gcgcgtgaac gccggatgct ccgacggctt cacatattgg cggagcgcga
     2401 tgacgctcgg gatcggcatc gtccgcgtcg gcgaatggat ttcgcgctcg taattttcga
     2461 cgatggtaac gcggtcgaga aaaaccgcct tcaccgcggt ctgccagggc cagaggctca
     2521 gcggataata gctcagcggc gtgtaatcgg cgttgagaac gagcgccgga cagctatcgg
     2581 gatgccgggc aagatcggga tggaacatgg ctaaataacg ctgcccccat tgcttcacgg
     2641 gatggcgcgc cactgcctcc cgcgcgtgac accatcatta catgccgcat cagattctga
     2701 cgtcaagggg cttgtgcccc gcaagatatg gaatgattgg ctttgactcg gcacagcata
     2761 tggattcaag gaccggaaga tgctgacgcg tttcgccccc agcccgaccg gcgagctcca
     2821 cctcggccac gcctatagtg cggttttggc gcacggcgcg gcgcgcgctg cggacgggcg
     2881 cttccgtatc cgcatcgacg acatcgacgg cagccgctcg cgcgaagcgt ttgtcggcgc
     2941 gtcgctcgcc gacctcgcat ggctcgggat cgactgggac ggcgacccgg tgc
//
LOCUS       DATMSU010000456         7604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_74191, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000456 DATMSU010000000
VERSION     DATMSU010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74191"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2217
                     /locus_tag="VN034_12215"
     CDS             <1..2217
                     /locus_tag="VN034_12215"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HWV61417.1"
                     /translation="VGTAPLDDPAVIGNAPPPGEPVYSGQTSPGARLPHGSVEAAPVN
                     NNIELLYPNGVPVPYYYTNPTMIGFTGGGGSTYGGGAEPIVMPVVGDWSPPPAYSQSY
                     YDKSREKASPGYYSVYLDTFRTQVELTATRWASVMRFTFPASDRSNVIVNLPRHGGTV
                     EVVGDRIVRGISKDEHSADGAYFVAEFSKPFAALGTFRKSPGDNIGWGIGTSDVQPGA
                     RDIAGSYAGGYVTFKTTAGEQVLMKIAHGTSYEQAAERLAAEVPGWDFDGVHKAARDT
                     WAQLLNRVRVTGGTEKQRKLFYSTLFQSFASPRLIAQKGEKFADTNGKVQTATHDRYG
                     PVPFWDTGRNQIVLLELMEPEAVKNIMASELAMAREKGYMNTSFHGDNAVFLFLGAWE
                     RGIEFDYEAAYEFMRKNATDPKGPRGYLAEYMKQGWISDIVPDHNPSPPYAGGKAGAD
                     KTLEYAWDDYALALYAKKLGKDADYRMFLKRAGNYANVFDKSIGFMRGRTADGKWIAP
                     FDPQEPYYNFMMKEASGWSTLWLVPHDVQGLIGLLGGRDAFNAKLDEFFTKPYNPTGI
                     CRDCTGVIGQYVHGNQPDQQAPYYYNWSGQPWKTQALVRKILEKTYASDAAGYGYPGM
                     DDQGATSSWYALSALGFYPVDPSSQDYIIGSPVFDDASLDMGNGKTLRIVAKNNSDTN
                     MYIQSATLDGKPWNKPWFRHDDVKDGATFVFTMGPKPNKAWGASPDAAPPSMSRKR"
     gene            2312..4273
                     /locus_tag="VN034_12220"
     CDS             2312..4273
                     /locus_tag="VN034_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HWV61418.1"
                     /translation="MRSLLITASLLALAPLAAQAQTAASPATASGGDAVYTGELMTRW
                     GKEVTPENAWRLYPRPQMVRERWVNLNGLWDYAIAPKAADRPAAMDGKILVPFPVESK
                     LSRVARKVTPDDRVWYRRSFTVPADWRGENVMLNFGAVDYETTVSVNGSVVGSHKGGF
                     DAFGFDITPYLKEGANELVVEVLDPTSAGTQPRGKQILDPAGIWYTAVSGIWQTVWLE
                     PVPKLHIAEVRATPDIDRGTVDVAVALSGWANDTDAVRLTASAGGKLIASTIIRANRR
                     ATLAIPNAHLWSPSDPHLYDLKAELVTIADPYAGEAERDRKAYDSRFTDHESRVYAKA
                     QVQGAPRDSVAAYFAMRKVSIGPGRVAGQPAILLNNKPLFQHGTLDQGWWPDGLYTPP
                     SEEAMKSDIVFLKKAGFNMLRKHIKVEPARYYHDADRLGMLIWQDMPSGGGEDQYLAG
                     TSQYQAIFPSETLAEQQKQLSEMVGELRAFPSIVTWVVNNEGWGQYDSETLARFVKGL
                     DPSRLVNANSGWVDVAPGISEMLDIHTYEDVPRTPPLQTNRAIVLGEYGGVGFPVPGH
                     IWKPGKGNWSYQVAKDEKEYLVRFRRKMEGVIRQAKENGLSASVYTQTTDVEDEINGL
                     LTYDRAVPKAPAEALADIAAPLWTTPAPD"
     gene            4566..7559
                     /locus_tag="VN034_12225"
     CDS             4566..7559
                     /locus_tag="VN034_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61419.1"
                     /translation="MKAGQIHSAKTNRRAALVRELMLSAGAMAALCGAGQAHAQAAPE
                     APPADDAAEAPGDAIVVTGYRQSIEQSLEQKREANSLIEVITAEDIGKFPDKNVADAL
                     QRVPGVIITRDGGEGSRVSIRGLQSDLTLTLLNGNFIAGADSGDPSRSFNYVLLPSNF
                     IASTEVYKSAEARLEEGGVGGTVILNTRRPFDLPAWSGFISAEGTYSDTTKKFDPQIA
                     GQISWKDPEERLGFLVGATYQERSNRELRGSTETWRWWSDRDEDGNILTPATDVNGNP
                     FENDDAISYWPGEGVTAQDGTHYSGHWAPQSVNAEVFSQHRKRLGIQATAQMRPFDNL
                     TVTANYFRFDYSSNFQSNVLKIPEWGYGNFFTGPTFDESGTIFQSANFQVPAAGTGCL
                     TNETPCTMETPQIAGTYSREKQVSNTFETEVAWNTDNFDAVAKFGKTRATGGPSMRFG
                     VAVKPRLTIPGEEQNGNFLSAWDFTGGNLNMEFSPELQENIKNGIAQIDVGSTGSGFT
                     NSAITQRYAQLDLVRRFDSILSALRVGGKWREMSIHRETGRNEWYADPATKLRYQDTP
                     EGAVARPEYFYDNPIGNIAGGFDASSFPGINFENYLDYINNTYGPSVRVPEPNNTYDL
                     KEKVFAGYVQADFEAGPLRGNIGVRVAHTKQSGLTSDRLQFLNDYCVDGPGGPFDPDV
                     PLAPDGNCQVRPLDEREVIVNTRIDQSKSYTDWLPSLNVVYEVTPDIVIRGAVAKVIS
                     RPSFNDLGSQRSLTYRSAAYAFDRGQFGEFEGWSGSGGNADLQPFSAWQYDLGVEWYF
                     KRGSVLGVGLFRKDVSDFVVPLVLDVTREVNGEQVLIQPYSTVANGSNAVSQGVEVYA
                     QHTLDFGLGAQVNFTYNDTSVADITLDGETVGKSALVGSAKTQVNASVFYETDKFLVR
                     ASYNRRGEVVGGLASGLNVYTDPYEQVDINASYELMDGLMLTASVINLTKSEERQHLG
                     NDTKARFVRSNYFGRRAYVGVSYKF"
BASE COUNT         1481 a         2481 c         2360 g         1282 t
ORIGIN      
        1 gtcggcaccg cgccgctcga cgatccggcg gtgatcggca acgcaccgcc gccgggcgag
       61 cccgtctatt cgggacagac ctcgccgggc gcgcggctgc cgcacggatc ggtcgaggcg
      121 gcgccggtca ataacaatat cgagcttctc tatccgaacg gcgtccccgt tccctattat
      181 tataccaatc cgacgatgat cggcttcacc ggcggcggtg gctcgaccta tggcggcggc
      241 gccgagccga tcgtcatgcc ggtggtgggc gactggtcgc cgccgcccgc ctacagccaa
      301 tcctattacg acaagtcgcg cgaaaaggcc tcgccgggct attattcggt ctatctcgat
      361 accttccgca cccaggtcga actcaccgcg acgcgctggg cgagcgtgat gcgcttcact
      421 ttccccgcga gcgatcggtc gaacgtcatc gtcaacctgc cgcgccacgg cgggacggtc
      481 gaggtcgtcg gcgaccgcat cgtgcgtggg atatcgaagg atgagcacag cgccgacggc
      541 gcctatttcg tcgccgaatt ctcgaagccc ttcgccgcgc tcggcacctt ccgcaagtcg
      601 cccggcgaca atatcggctg gggcatcggc accagcgacg tgcagcccgg cgcgcgcgac
      661 atcgcgggca gctacgccgg cggctatgtg accttcaaga ccacggccgg cgagcaggtg
      721 ctgatgaaga tcgcgcacgg caccagctat gaacaggcgg ccgagcggct cgccgccgaa
      781 gtgccgggct gggatttcga cggcgtccac aaggcggcgc gagacacctg ggcacagctt
      841 ttgaaccgcg tccgcgtgac gggcggcacc gagaaacagc ggaagctctt ctactcgacg
      901 ctgttccagt ccttcgcctc gccgcggctg atcgcgcaga agggcgagaa gttcgccgac
      961 accaacggca aggtccagac cgcgacgcac gaccgctacg gtccggttcc cttctgggac
     1021 acggggcgca accagatcgt gctgctcgaa ctgatggagc ccgaggcggt caagaatatc
     1081 atggcttccg aactcgcgat ggcgcgcgag aagggctata tgaacacctc cttccacggc
     1141 gacaatgcgg tgttcctctt cctcggcgcg tgggagcgcg gcatcgagtt cgattatgaa
     1201 gccgcctatg aatttatgcg caagaatgcg accgatccca agggaccgcg cggctatctt
     1261 gccgaatata tgaagcaggg ctggatttcg gacatcgtgc ccgatcataa tcccagcccg
     1321 ccctatgccg gcggcaaggc gggcgccgac aagacgctcg aatatgcgtg ggacgattat
     1381 gcgctcgcgc tctatgcgaa aaagctcggc aaggacgccg actaccggat gttcctgaag
     1441 cgcgcgggca attacgccaa tgtcttcgac aaatcgatcg gcttcatgcg cgggcgcacc
     1501 gccgacggca aatggatcgc gccgttcgat ccgcaagaac cctattataa tttcatgatg
     1561 aaggaggcgt cgggttggtc gacgctctgg ctcgtcccgc atgatgtgca ggggctgatc
     1621 ggcctgctcg gcgggcgcga cgcgttcaac gccaagctcg acgaattttt caccaaaccc
     1681 tataatccga cgggtatctg ccgcgactgt accggcgtga tcggccaata tgtccacggc
     1741 aaccagcccg accagcaggc cccctattat tacaattgga gcggccagcc gtggaagacg
     1801 caggcgctgg tccgcaagat cctcgagaaa acctacgcca gcgacgccgc gggttacggc
     1861 tatccgggga tggacgatca gggcgcgacc tcgtcctggt atgcgctttc ggcgctcggc
     1921 ttttaccccg tcgatccgtc gagccaggat tatatcatcg gcagtcccgt gttcgacgac
     1981 gccagcctcg acatgggcaa tggcaagacg ctgcgcatcg tcgcgaagaa taattcggac
     2041 acgaatatgt acatccagtc ggcgacgctc gacggcaaac cttggaacaa gccatggttc
     2101 cgccacgacg acgtcaagga cggcgcgacc ttcgtcttca ccatggggcc caagcccaac
     2161 aaggcatggg gcgcgtcgcc cgatgcggcc ccgccgtcga tgtcgcgaaa gcgctaacca
     2221 gcccctcgcc cccgtgtcct ccgcgggggc gcgagcccgg gatccgcctt cccccttggg
     2281 tcccgggctc ccctcttcaa caggagatac catgcgcagc cttctgatca ccgcatccct
     2341 tctcgctctc gcgccgctcg cggcacaggc ccaaaccgct gcatcgcctg ccacggcgtc
     2401 cgggggcgac gccgtctaca ccggcgagct catgacgcgc tggggcaagg aggtgacgcc
     2461 cgaaaacgcg tggcgcctct acccgcgccc gcagatggtg cgcgagcgct gggtcaattt
     2521 gaacggcctg tgggattatg cgatcgcgcc caaggcggcc gacaggccgg cggcgatgga
     2581 cggcaagatc ctcgtgcctt tcccggtcga atcgaagctg tcgcgcgtcg cgcgcaaggt
     2641 cacgcccgac gaccgcgtct ggtatcgccg cagcttcacc gtccccgccg actggcgcgg
     2701 cgagaacgtc atgctcaact tcggcgcggt cgattatgag acgacggtga gcgtcaacgg
     2761 ctcggtggtc ggctcgcaca agggcggctt cgacgccttc ggcttcgaca tcacccccta
     2821 tctgaaggag ggcgcgaacg agctcgtcgt cgaggttctc gacccgacga gcgcgggcac
     2881 ccaaccgcgc ggcaagcaga tcctcgaccc agccggtatc tggtacacgg cggtcagcgg
     2941 catctggcag acggtgtggc tcgaaccggt gccgaaactc catatcgccg aggtgcgcgc
     3001 gacgcccgac atcgaccgcg gcaccgtcga tgtcgcggtg gcattgagtg gctgggctaa
     3061 cgataccgac gcggtgcggc tgaccgcgag cgcggggggc aagctgatcg cctcgacgat
     3121 catccgcgcc aaccggcggg cgacgttggc gatcccgaac gcgcatctct ggtcgccgag
     3181 cgatccccat ctctatgacc tcaaggccga actggtgacg atcgccgatc cctatgcggg
     3241 cgaagccgag cgcgaccgca aggcctatga ctcgcgcttc accgatcatg aaagccgcgt
     3301 ctatgccaag gcgcaggttc agggcgcgcc acgcgacagc gtcgccgcct attttgcgat
     3361 gcgcaaagtg tcgatcggac ccggccgcgt cgcgggacag cccgcgattc tgctcaacaa
     3421 caagccgctc ttccagcacg gcacgctcga tcagggctgg tggcccgacg ggctctacac
     3481 gccgccgtcg gaggaagcga tgaagagcga catcgtcttc ctgaagaagg cggggttcaa
     3541 tatgctgagg aagcatatca aggtcgaacc cgcgcgctat tatcacgacg ccgaccggct
     3601 cggcatgctg atctggcagg acatgccctc gggcggcggc gaggatcaat atcttgccgg
     3661 caccagccag tatcaggcga tcttcccttc ggagacgctg gccgagcagc agaaacaatt
     3721 gtccgaaatg gtcggcgagc tgcgcgcttt cccgtcgatc gtgacatggg tcgtcaacaa
     3781 tgaagggtgg ggccaatatg attcggaaac gctcgcacgc tttgtgaagg ggctcgatcc
     3841 ctcgcggctc gtcaatgcca attcgggctg ggtcgacgtc gcgcccggca tatccgaaat
     3901 gctcgatatc cacacctatg aggatgtgcc gaggacgccg ccgctccaga cgaaccgcgc
     3961 gatcgtgctc ggcgaatatg gcggcgtcgg cttccccgtg ccgggccata tctggaaacc
     4021 cggcaagggc aattggagct atcaggtcgc caaggacgag aaggaatatc tcgtccgctt
     4081 ccgccgcaag atggaaggcg tgatccgcca ggcgaaggag aatgggctca gcgcttcggt
     4141 ctacacccag acaaccgatg tcgaagacga gatcaacggg cttttgacct atgaccgcgc
     4201 ggtgccgaaa gcgccggcgg aggcgctggc ggacatcgcc gcgccgctgt ggacgactcc
     4261 ggcgcccgac tgacgggcga ggagcgaaaa gcgagacggc atccgccatc ccgggccggg
     4321 gatggcgggt gtcgagtcgt gcggcagctc gtctggccgc aagaaacgac gaaaaactgc
     4381 ggatttttga tggatcgaca acgattcatc gcgtcccgcc accggctcga aaaaattatt
     4441 ttgacaaatt gttttttata ttctgataga ggctcaacgg gtccggggat ccgctgctcg
     4501 gcgactccga aagcgccgga taacaagcgg gggcgaacag cccctcgaac agggaggtcg
     4561 atgtgatgaa ggcaggacaa atccattcag cgaagacgaa tcggcgcgcg gcgctggtgc
     4621 gcgagctgat gctgagtgcg ggcgcgatgg ccgcattgtg cggggcgggg caggcgcacg
     4681 cacaggcagc cccggaagca ccgccggcgg atgacgcggc agaggccccc ggcgacgcga
     4741 tcgtcgtcac cggctatcgc cagtcgatcg aacagagcct tgagcagaag cgcgaagcga
     4801 attcgctgat cgaggtcatc accgccgagg acatcggcaa attccccgac aagaatgtcg
     4861 ccgacgcgct gcagcgcgtc cccggcgtga tcatcacccg cgacggcggc gagggcagcc
     4921 gcgtcagcat tcgcggtctc cagtcggatt tgacgctgac cctgctcaac ggcaatttca
     4981 tcgcgggcgc cgacagcggc gatccgtcgc gctcgttcaa ctatgtgctg ttgccgtcga
     5041 acttcatcgc cagcaccgaa gtctacaaat cagccgaggc gcggctcgaa gagggcggcg
     5101 tcggcggcac ggtgatcctc aacacccgcc gtccgttcga cctgccggcc tggtcgggct
     5161 tcatctcggc cgaaggcacc tattcggaca cgaccaagaa attcgacccg cagatcgccg
     5221 gccagatttc gtggaaggac cccgaagaac ggctcggctt cctcgtcggc gcgacctatc
     5281 aggagcgttc gaaccgcgaa ctgcgtggct cgaccgaaac ctggcgctgg tggagcgacc
     5341 gcgatgagga cggcaacatc ctgaccccgg cgaccgacgt caacggcaac cccttcgaaa
     5401 acgacgacgc gatttcctat tggccgggcg agggcgtgac cgcgcaggac ggtacgcatt
     5461 attcgggcca ttgggcaccg cagtcggtca atgccgaagt cttctcgcag caccgcaagc
     5521 gcctcggcat ccaggcgacc gcgcagatgc gtccgttcga caatctgacc gtcaccgcca
     5581 attatttccg cttcgactac agcagcaatt ttcagagcaa tgtgctgaaa atccccgaat
     5641 ggggctatgg caatttcttc accgggccga ccttcgacga gagcggcacg atcttccaat
     5701 cggcgaattt ccaggtgccc gccgcgggga ccggctgtct gaccaatgag acgccgtgca
     5761 cgatggaaac gccgcagatc gcgggcacct atagccgcga aaagcaggtg tcgaacacct
     5821 tcgagaccga ggtcgcctgg aataccgaca atttcgacgc ggtagcgaaa ttcggcaaga
     5881 cgcgcgcgac cggcggcccg tcgatgcgct tcggcgtcgc cgtgaagccg cgcctgacga
     5941 tccccggtga ggagcagaac ggcaacttcc tgagcgcctg ggatttcacc ggcggcaatc
     6001 tcaacatgga attctcgccc gagctccagg agaatatcaa gaacggcatc gcccagatcg
     6061 acgtcggctc gacggggtcg ggcttcacca acagcgcgat cacgcagcgt tatgcccagc
     6121 tcgacctcgt ccgccgcttc gacagcattt tgagcgcgct gcgcgtcggc ggcaaatggc
     6181 gcgagatgag catccatcgc gaaaccggcc gcaacgaatg gtatgccgat ccggcgacca
     6241 agctgcgcta tcaggacacc cccgagggcg cggtcgcgcg gcccgaatat ttctatgaca
     6301 atccgatcgg caacatcgcc ggcgggttcg acgcgagctc gttccccggc atcaatttcg
     6361 aaaattatct cgactatatc aacaacacct atggcccgtc ggtgcgcgtg cccgaaccga
     6421 acaacaccta tgacctcaag gaaaaggtgt tcgccggtta tgtgcaggcc gatttcgagg
     6481 ctggtccgct gcgcggcaac atcggtgtcc gcgtcgcgca caccaaacag tcgggcctga
     6541 cgtcggatcg cctgcaattc ctcaacgatt attgcgtcga cggtccgggc ggcccgttcg
     6601 atcccgacgt gccgctcgct cccgacggca actgccaggt gcggccgctc gacgagcgcg
     6661 aagtcatcgt caacacgcgc atcgaccagt cgaagagcta caccgactgg ctccccagcc
     6721 tgaacgtcgt ttacgaagtc acccccgaca tcgtcatccg cggcgcggtc gccaaggtga
     6781 tctcgcgtcc gtcgttcaac gatctggggt cgcagcgttc gctgacctat cgttcggcgg
     6841 cctatgcctt cgatcgcggc cagttcggcg aattcgaagg ctggtcgggc agcggcggca
     6901 acgccgatct ccagcccttc tcggcatggc aatatgacct tggcgtcgaa tggtatttca
     6961 agcgtggttc ggtgcttggc gtcggcctgt tccgcaagga cgtgtccgac ttcgtcgtcc
     7021 cgctcgtgct cgacgtgacg cgcgaagtga atggcgagca ggtgctgatc cagccctatt
     7081 cgaccgtcgc caatgggtcg aacgccgtgt cgcagggtgt cgaagtctat gcccaacata
     7141 cgctcgactt cggcctcggc gcgcaggtga acttcaccta caacgacacg tcggtcgccg
     7201 acatcacgct cgacggcgag acggtcggca agtcggcgct cgtcggcagc gccaagacgc
     7261 aggtcaacgc ctcggtcttc tatgaaaccg acaagttcct cgtccgcgcc tcgtacaacc
     7321 gccgcggcga agtcgtcggc gggctcgcct cgggcctcaa cgtctacacc gacccctatg
     7381 aacaggtcga catcaacgcg tcgtacgagc tgatggacgg gctgatgctc accgcctcgg
     7441 tcatcaacct gaccaagtcg gaagagcgcc agcaccttgg taacgacacc aaggcgcgct
     7501 tcgtccgcag caactatttc ggccgccgcg cttacgttgg cgtttcgtac aagttctgat
     7561 ggggcccata ccccagctct cccccttggg tggctccgca agga
//
LOCUS       DATMSU010000457         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_168377,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000457 DATMSU010000000
VERSION     DATMSU010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168377"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1420
                     /locus_tag="VN034_12230"
     CDS             17..1420
                     /locus_tag="VN034_12230"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWV61420.1"
                     /translation="MLEQLADHDDILLEQLLNDEKPSFDTIFGDLARETAAGQIVPIL
                     FGSALNGFGIRRLLKMLRHDTPPAALTADRLAIDGAAAQVFKVTHGSAVGRLALARVF
                     GAPLAEGAELLGADGNPMRIGSLFAVQGAATTKIARAEPGDIVGIAKVDVVQAGDRLG
                     AAGHPHAVVDAVQRPASNYALAIAVTDHKDEVRLSTALNRLVEEDPGLRWRLDEATQE
                     TLLHGLNDEHLAVVQARLKRRYGVAISVRPPAIAYKETIRKTVTKHGRHKKQSGGHGQ
                     FGDVIIELRPLKRGDGFQFEDRITGGAIPRQWIPAVEQGVRDAMARGPFGFPVVDVAV
                     ALVDGSFHSVDSSELAFRTAGRIAMAEALAAATPYLLEPLAHVTIRAPGSAVSRITSA
                     ATSRRGQVLGITPLEGWSRWDLIEVLLPEAELPGLEPELRSLSQGMAHYEAHFDHLAE
                     LSEKLASALIRRAPESA"
     gene            complement(1471..3825)
                     /locus_tag="VN034_12235"
     CDS             complement(1471..3825)
                     /locus_tag="VN034_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61421.1"
                     /translation="MKRIACTSLAAFAVALAAMPAAAQEAKPADTAPDMGDAIVVTAQ
                     KRAENIQDVPISIAAVSGETMESANILTVMDLGRVATNFQTVRSSNTGSTRIGIRGVG
                     SLANTLIEPSVAVFVDGVYVPRSGSILGAFLDIDGVEVLRGPQGTLFGRNASVGALSI
                     RSALPEDEFSGAVSAEAGSFDRYKLSGHINVPLGENVSARVAAMGQWYDGPWKNVYDG
                     KTYGGSDDVAVRGTVKAEFGNLEWIVRGDYTRLDGDGVVNFDFDPASVAPARLAFLRA
                     AFSGGPDTNLNDRVMNQSVASGLLDKNWGVSSTASLEIGGGSTLRLVNSYRKWTNDQL
                     DGDIIFLPVPLATRRSLFDSGSQNHELQFISPEREWLGGHFDMVAGLYYFQEKFALGE
                     QLNMDTQFCNILVPAGPGRTACAAYYANTGGIAATDQDVYQKVRSIAAYAQGNIYLSD
                     ALTLTLGGRWTNDRKRGRYSQLSSPFTQTIRAPEVLDYPGLSESRFTYRVSLNYKPAD
                     DVMLFATHSTGYKSGGYNSGGGVPALSTFDPQGNLISTRRLFDRETVKNYEFGAKTSW
                     LDRALTANITFFRMDISGFQDRAFDGVSFVVRNAGNLRQQGFELDLKLAPSDRFSLSG
                     SLAYLDSEFTDFAGASGLPGIVGTQDLTGKPNSFSPPWSGNVAVDWNGDVGSSGWTWA
                     ANANVSFVSDQYVGTVNDANPQSIADGYALLGARLTLSGPDDRWSLAIFGSNLTNTHY
                     RPLAVYQPLGAALGLNNGAFAGSTANRILASEPRTYGVSATFRF"
BASE COUNT          674 a         1216 c         1310 g          670 t
ORIGIN      
        1 cgatgcgcgc ttccacatgc tcgaacagct ggcagatcat gacgatatac tgctcgagca
       61 attgctgaac gacgagaagc caagcttcga cacaatcttt ggcgatctcg cccgtgagac
      121 cgcggcgggg cagatcgtac cgatcctgtt tggctcggcg ctcaacgggt ttggcatccg
      181 ccgtctcctc aaaatgctgc gccatgatac accccccgcc gcgctgacgg cggatcggct
      241 tgctatcgac ggtgccgcgg cgcaggtgtt caaggtcacg catggcagtg cggtgggccg
      301 gctggcgctc gcgcgtgtct tcggtgcgcc gcttgccgag ggggccgaac tgttgggcgc
      361 cgatgggaac ccgatgcgta tcggatcctt gttcgcagtg cagggagcgg cgaccacgaa
      421 gatcgctcgc gccgaaccgg gcgacatcgt cggcatcgct aaggtggatg tcgtccaggc
      481 gggcgaccgg ctcggcgcag ccggtcaccc gcatgctgtc gtggacgcgg tgcagcggcc
      541 ggcaagcaat tatgcgctgg ccatcgcggt caccgaccat aaggacgagg tgcggttgtc
      601 gacagcgctc aatcgactgg tcgaggagga ccccggactg cgctggaggc tcgacgaggc
      661 gacgcaggag acgctgctcc acgggctcaa tgacgagcat ctggccgtcg tccaggcacg
      721 gctgaaacgg cgttacggcg ttgccatatc ggtgcgtccg cccgcgatcg cctacaagga
      781 aacgatccgc aagaccgtga caaagcatgg ccgccacaaa aagcaatccg gcgggcacgg
      841 ccagttcggc gacgtcatca tcgaactccg cccactcaag cgtggcgacg gctttcagtt
      901 cgaggaccgg atcaccgggg gcgcgatccc ccggcaatgg attccggcgg tcgagcaggg
      961 cgtgcgcgat gcgatggcgc gcggaccatt cggctttccg gtcgtcgatg tcgcggtcgc
     1021 gctggtcgat ggctcctttc acagcgtcga cagctcggaa ctcgcttttc gcaccgccgg
     1081 tcggatcgcc atggcggaag ccctggcggc ggcgacgccc tatctgctcg agccgctcgc
     1141 gcacgtgacc atccgggcgc cgggcagcgc cgtttcacgg atcacctccg cggcgaccag
     1201 ccgacgcggc caggtgctgg gaataacgcc gctcgaaggc tggtctcgct gggacctgat
     1261 cgaggtgctg ctgcccgagg ctgaacttcc cggactggag cctgaactgc ggtcactgag
     1321 ccagggtatg gcgcattacg aggcgcactt cgaccatctc gcggaactca gcgagaagct
     1381 cgccagcgcg ctgatccgcc gcgcgcccga gtccgcctga cccgaaatgg ccgggcacga
     1441 agtcgctagc aacgtgccgc ggcagtagct tcaaaaccgg aacgtggcgc tgacgccata
     1501 agttcggggc tcgctcgcga gaatgcggtt tgccgtgctc ccagcgaaag cgccgttgtt
     1561 gagaccgaga gctgcaccaa gcggctggta gacggcgagg gggcgataat gggtgttggt
     1621 gaggttgctg ccgaagatcg cgagcgacca gcggtcgtcg gggccgctca acgtcagccg
     1681 cgcgccgagc agggcatagc cgtcggcgat cgattgcgga ttggcgtcgt tgaccgtgcc
     1741 gacatattgg tcggacacga aggagacgtt ggcgttcgcc gcccatgtcc atccgctcga
     1801 tccgacatcg ccgttccagt cgacggcgac attgcccgac caagggggcg agaaactgtt
     1861 gggcttgccg gtcaggtcct gcgtcccgac gatgcccggt aggcccgagg cgcccgcgaa
     1921 atcggtgaat tcggagtcga gataggcgag cgagcccgag agcgaaaagc ggtcgctggg
     1981 cgcgagcttc aggtcgagtt cgaacccctg ctggcgcagg ttgccggcgt tgcggacgac
     2041 gaagctgacc ccgtcgaagg cgcgatcctg aaagcccgat atgtccatgc gaaagaaggt
     2101 gatgttcgcg gtcagcgcgc gatcgagcca gctcgtcttg gcgccgaact cgtaattctt
     2161 gaccgtctcg cggtcgaaca ggcgccgcgt cgagatcaga ttgccctgcg ggtcgaaggt
     2221 cgagagcgcc ggcacgccgc cgcccgaatt atagccgccc gacttgtagc ccgtcgaatg
     2281 ggtagcaaag agcatcacgt cgtcggcggg cttgtagttg aggctgacgc gataggtgaa
     2341 gcggctttcc gacagccccg gatagtcgag cacctcgggc gcgcggatcg tctgggtgaa
     2401 ggggctcgat agctgtgaat agcggccgcg cttgcggtcg ttggtccagc gcccgccgag
     2461 cgtcagcgtc agcgcgtccg aaagatagat attgccctgc gcataggccg cgatgctgcg
     2521 gaccttctga tacacgtcct gatcggtcgc ggcgatgcca ccggtgttcg cataataggc
     2581 cgcgcatgcg gtgcgtcccg gccccgcggg gacaaggatg ttgcagaact gcgtgtccat
     2641 gttgagctgc tcgccgagcg cgaatttctc ttggaaataa tagaggcccg cgaccatgtc
     2701 gaaatggccg cccagccatt cgcgttcggg cgagatgaat tgcagctcgt gattctggct
     2761 gcccgaatcg aacaggctgc gccgcgtcgc gagcgggacg ggcaggaaga tgatgtcgcc
     2821 gtcgagctgg tcgttcgtcc atttgcgata gctgttgacg aggcgcagcg tcgatccgcc
     2881 gccgatctcg agcgatgctg tgctcgacac gccccaattc ttgtcgagca gtcccgaggc
     2941 gaccgactgg ttcatcaccc ggtcgttcaa attggtgtcg gggccgccgc tgaacgccgc
     3001 gcgcagaaag gcgagccgcg ccggggcgac cgaggcgggg tcgaagtcga aattgaccac
     3061 gccgtcgccg tcgagccgcg tataatcgcc gcgcacgatc cattcgagat tgccgaactc
     3121 ggccttcacc gtgccgcgca ccgcgacgtc gtcggacccg ccataggtct tgccgtcgta
     3181 aacgttcttc cacggcccgt cataccattg ccccatcgcc gcgacgcgtg ccgagacatt
     3241 ctcgccgagc ggaacattga tgtggcccga cagcttgtag cggtcgaacg agccggcttc
     3301 ggccgagacc gcacccgaaa attcgtcctc gggcagtgcg ctgcggatcg acagcgcgcc
     3361 gacgctggcg ttgcgtccga acagcgtgcc ctgcgggccg cgcagcacct cgaccccgtc
     3421 gatgtcgagg aaggcgccga ggatcgagcc cgagcgcggg acatagacgc cgtcgacgaa
     3481 caccgcgacg ctcggttcga tcaacgtgtt ggcgaggctg ccgacacccc ggatgccgat
     3541 gcgcgtcgag ccggtgttcg acgagcgcac cgtctggaaa ttggtcgcga cgcggccgag
     3601 gtccatcacg gtgaggatgt tcgcgctctc catcgtctcg ccgctgaccg ccgcgatcga
     3661 gatcggtacg tcctggatat tttcggcgcg cttctgtgcg gtgacgacga tcgcgtcgcc
     3721 catgtcgggc gcggtgtcgg cgggtttcgc ctcctgcgcg gcggcaggca tggccgcgag
     3781 tgcgacagcg aaggcggcaa ggctcgtgca ggcaatcctc ttcatatttc cctcccagga
     3841 tgatctattg gtgatctttg atcgaaggct
//
LOCUS       DATMSU010000458         9763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_26502, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000458 DATMSU010000000
VERSION     DATMSU010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 9763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9763
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26502"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..2209)
                     /gene="katG"
                     /locus_tag="VN034_12240"
     CDS             complement(8..2209)
                     /gene="katG"
                     /locus_tag="VN034_12240"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HWV61422.1"
                     /translation="MNDPTPIDPAGGCPIHQPGGVRALLGRTNKDWWPEMLATEILNP
                     NGSSNPLGDDFDYAKAFKSLDYQALKDDLTALMTDSQPWWPADYGHYGPFFIRMAWHA
                     AGTYRTADGRGGANSGQQRFAPLDSWPDNGNLDKARRLLWPIKQKYGNKISWADLFIL
                     AGNVAIESMGGPVFGFGGGRVDVFEPERDIYWGSEDKWVNQGVQTRIAPEHGMHEIEG
                     PLAAIQMGLIYVNPEGPQGNSNDNEGMARDMKETFARMAMNDEETVALTAGGHTFGKA
                     HGNGDASLLGNAPSGGDLVAQGFGWVSGHESGGIGEHTVTSGLEGAWTNTPDKWTDNF
                     FRLLFDYEYELVQSPAGANQWQPIDQKEEDMAPAAWDPNLKVPTMMTTADMALKRDPA
                     YRKISEKFRDDHDAFRDAFARAWFKLTHRDMGPKVRYLGPEVPAEDLIWQDPIPAGST
                     PSDADVKAVKDKIAASGLTVSQLIKTAWASASTYRKSDFRGGANGARVRLAPQKDWEV
                     NEPAMLAKVLDTLGGLRGSLSMADAIVLGGVVGLEKAIKDAGFSVAVPFTGGRGDATD
                     EQTDADSFAVMEPEADAFRNYLGSKKLAVKTEEMMLDRASLLGLSVPEMTVLIGGLRV
                     LGANHGERGHGHFTKRSGQLTNDYFVNLLDMTNVWKAVDGSDDQEYVATDRKGGGETW
                     RATRADLIFGSNSELRAVAEVYAESGHEEKFVKDFVKAWTKVMNADRFDLA"
     gene            2566..2721
                     /locus_tag="VN034_12245"
     CDS             2566..2721
                     /locus_tag="VN034_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61423.1"
                     /translation="MANNPNQQNEKKPQADRDEEQRRQQQQQGGQDRQQGGQRSDQDR
                     NKNPQQR"
     gene            complement(2802..5225)
                     /locus_tag="VN034_12250"
     CDS             complement(2802..5225)
                     /locus_tag="VN034_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61424.1"
                     /translation="MTAKYPSVRRRVPASLFTLSLALAFAASPAAAAETADNTAAADD
                     AAAQVEGGSYGGEIIVTARRRQETAQDVPIAISVVDGEQIDNTGSFNVGRLTQLTPTL
                     QFYSSNPRNTAVNIRGIGAPFGLTNDGIEQGVGIYVDDVYYSRVASSTFDFLDVAQIE
                     VLRGPQGTLYGKNTTAGAINITTNQPTFEFEGKAEVSIGNLDFKQAKAAISGPLSDQL
                     AARIAVSSTSRRGTIYNVTTDRYIQSQDNIGIRGQLLWRPAENLDITLAGDWNKQEAV
                     CCGSVFVRTGETQRPPNRQFAALAAAQGYAFPSDNPYDRLTDLDASLNAGNEIGGASL
                     RVKWDVGPGTLTSVTAWRYWDWQPENDRDFTGLPIVTRSQNPSQQDQYTQELRYNYTG
                     DRFDFVIGGFYYYQRIDTQGTEGHGPASSRWTINPSNPLSNDPSVLDGLTAINTQYLK
                     NTSAALFGQLSWKVTDQLTIQPGVRVNYDKKDGYYQRLVFAGDGSPVTADLTDAVSAA
                     RLGVFTPQEYAPSFSDWNFTYDLTATWKAARDLLFYATYAKTFKSGGINQNGVPNGAD
                     GDPILAAATVKPESVDHYEAGVKAEFWDRRATFNLSAFRTDIKNFQANVNNGQFGVLR
                     GYLANAGKVRTQGVEFDFSVRPSERFRAYANGAYTDAKYERFVDAPCPPELSGGTASA
                     PNCDISGQRLPGVSKWAFSFGAEANAESSLFGQDGQFYLGYDGSYRSDWSSNPSPSAY
                     TWIEGTSLSNFRAGFRTESGLDIFGWVRNAFDQNYLEQLSVGPGNTGLITGLPGDPRT
                     WGGTIKASF"
     gene            complement(5329..5499)
                     /locus_tag="VN034_12255"
     CDS             complement(5329..5499)
                     /locus_tag="VN034_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HWV61425.1"
                     /translation="MSAINEVAKGGSEEVPRAIQTVLDALDKLKFGAIQLTVHEGRLV
                     QVDVTERHRYPN"
     gene            5716..6720
                     /locus_tag="VN034_12260"
     CDS             5716..6720
                     /locus_tag="VN034_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWV61426.1"
                     /translation="MSKINDLMARGTELLGSDYAILCGAMSWVSERHLVSAISNAGGF
                     GVIACGAMTPALLDAEIAGTKALATRNFGVNLITMHPQLFELIEVCAKHRVGHVVLAG
                     GLPPKGSLEAIKASGAKVICFAPTLALAKKLVRSGVDALVIEGMEAGGHIGPVSTSVL
                     AQEILPTLADEVPVFVAGGIGRGEAIAAYLEMGASGVQLGTRFVCATESIAHPNFKKA
                     FMRASAREAVASVQIDPRLPVIPVRALKNAGTEAFTVKQREVANKLDKGEVDMMEAQL
                     EIEHYWAGALRRAVIDGDVENGSLMAGQSVGMVSEEESAAAIVASLVQQAEAALHARG
                     "
     gene            6753..7511
                     /locus_tag="VN034_12265"
     CDS             6753..7511
                     /locus_tag="VN034_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61427.1"
                     /translation="MNLSRKIIVAGLLASVAACAPKPPPPPPPPPPPPVVVTIPPRPL
                     PPGNAALTQILPGRGIDGRFVTANSGVTGDRAFWQLKIGLNVAAIGCRGIEETTLVSA
                     YNNIIKTHGKVIKSTEKSVIKELGKENKNNGIAPRDRLSTQLFNYFAQPPAQRAFCPR
                     ANEIAQLVSTTPTDQLVEQAPAHLARLDEPFKQFYEAYAQYQSDVIAWDAKYAPPPAI
                     MTTPVPATPIGPAITTGTTPVAPTTVPASPTGNP"
     gene            7598..>9763
                     /gene="ptsP"
                     /locus_tag="VN034_12270"
     CDS             7598..>9763
                     /gene="ptsP"
                     /locus_tag="VN034_12270"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HWV61428.1"
                     /translation="MTNAPPPPSSAAQSARTILTRLHEVMASRVNAQGKLNQVVGIIG
                     ECLDSEVCSIYLLRDGTLELYATRGLKQEAVHVTRLGLGEGLVGMIADQIETLNLDEA
                     AAHPDFSYRPETGEELFHSFAGVPIIRRERAVGVLCVQHADPRRYDDIEIETLQTVAM
                     VLSELIANADLVDTAARTDAAAADQSAQRLNGQKLVDGMGAGVAVFHQPRITIEHTVA
                     DDTEAERHRVYAAFDKMREQIDRMASSADFGVGGEHEEVIETYKMFAYDEGWSRRINE
                     AIDSGLTAEAAIERVQQRTRMRMRQIDDPLLRDRMHDLEDLSNRLIRIVSGQMGTAAQ
                     MGLRHDSILIARNLGPAELLEYDRRRLKGVVLEEGSLTAHVIIVARAMGVPVIGRVSD
                     VRSSIREGDLLLLDATAGQLHVRPTQAVQDAFDAKLEISQKRRANLASLRDMPAVTKD
                     GVPIELMINAGLREDVAALDLTGARGIGLFRTEFQFLVSATLPSRDRQQRLYRDVLDA
                     AGDRPVIFRTVDIGGDKALPYMNVDGTAQEENPAMGWRALRLALEREGLLKVQARALM
                     EAAAGRTLHVMFPMVSEPWEYEAARNLFVGQRAWLASHNKKLPVAIRYGAMLEVPGLL
                     ETLDLMLPHLDFLSVGTNDLTQFLFAADRAHPRLAERYDWLSPVVMRYLARIVRTVSG
                     SKVALGVCGEMGGRPLEAMALLGAGIERLSITPAGVGPVK"
BASE COUNT         1663 a         3110 c         3249 g         1741 t
ORIGIN      
        1 cagaagatca ggcgaggtcg aaacggtcgg cgttcatcac cttggtccac gccttgacga
       61 agtccttcac gaacttctcc tcatgcccgc tttcggcata gacctcggcg accgcgcgca
      121 gttccgagtt cgagccgaag atcaggtcgg cgcgcgtcgc gcgccaggtc tcgccgccgc
      181 ccttgcgatc ggtggcgaca tattcctggt cgtccgaacc atcgaccgcc ttccagacat
      241 tggtcatgtc gagcaggttg acgaaataat cgttggtcag ctggcccgac cgcttggtga
      301 aatggccgtg gccgcgttcg ccatgattgg cgccgaggac gcggaggccg ccgatcagca
      361 cggtcatctc gggaaccgac aggccgagca gcgaggcgcg gtcgagcatc atctcctcgg
      421 tcttcaccgc cagcttcttg cttccgagat agttgcggaa ggcatcggcc tctggctcca
      481 tcaccgcgaa gctgtcggcg tcggtctgtt cgtcggtggc gtcgccgcgg ccgccggtga
      541 agggcaccgc gacgctgaag cccgcgtcct tgatcgcctt ctcaagaccc accacgccgc
      601 cgaggacgat cgcgtcggcc atcgacagcg agccgcgcag gccgcccagc gtatccagca
      661 ccttggcgag catcgcgggc tcgttgactt cccagtcctt ttgcggggcg aggcggacgc
      721 gcgcgccgtt ggcgccgccc cggaagtcgg acttgcgata ggtgctcgcc gaggcccagg
      781 cggtcttgat cagctggctg actgtcaggc cgctggccgc gatcttgtcc ttcaccgcct
      841 tgacgtcggc gtccgagggc gtcgatccgg cggggatcgg gtcctgccag atcagatcct
      901 cggcgggcac ttcggggccg agatagcgga ccttcggacc catgtcgcgg tgggtcagct
      961 tgaaccacgc acgcgcgaag gcgtcgcgga acgcatcatg gtcgtcgcgg aacttctcgc
     1021 tgatcttgcg gtacgccggg tcgcgcttca gcgccatgtc ggcggtggtc atcatcgtcg
     1081 ggactttcag gttcggatcc caggccgcgg gggccatatc ctcttccttc tggtcgatcg
     1141 gctgccactg gttggcgccc gcgggcgact gcaccagctc atactcatag tcgaacagca
     1201 ggcggaagaa attgtcggtc catttgtcgg gcgtgttggt ccacgcgcct tcgagcccgc
     1261 tggtgacggt atgttcgccg ataccgccgc tttcgtggcc gctgacccag ccgaagccct
     1321 gcgcgacgag gtcgccgccc gagggagcgt tgccgagcag cgacgcgtcg ccattgccgt
     1381 gcgccttgcc gaaggtgtgg ccgccggcgg tcagcgcgac ggtttcctcg tcgttcatcg
     1441 ccatgcgcgc gaaggtttcc ttcatgtcgc gcgccatgcc ttcattgtcg ttcgaattgc
     1501 cctgcggccc ttccgggttg acgtagatga ggcccatctg gatcgcggcg agcgggcctt
     1561 cgatctcgtg catgccatgc tcgggggcaa tgcgcgtctg cacgccctgg ttcacccatt
     1621 tgtcctcgct gccccaatag atgtcgcgct cgggctcgaa cacgtcgacg cggccgccgc
     1681 cgaagccgaa caccggccca cccatgcttt cgatcgcgac attgccggcg aggatgaaca
     1741 ggtcggccca gctgatcttg ttgccatatt tctgcttgat cggccaaagc aggcggcgcg
     1801 ccttgtcgag attgccgttg tcgggccagc tgtcgagcgg tgcgaaacgc tgctgcccgc
     1861 tgttggcgcc gccgcggccg tcggcggtgc gataggtgcc cgccgcgtgc cacgccatgc
     1921 ggatgaagaa ggggccgtag tgaccatagt cggcgggcca ccagggctgg ctgtcggtca
     1981 tcagcgcggt caggtcatcc ttcagcgcct gataatcgag cgacttgaac gccttcgcat
     2041 agtcgaaatc gtcgccgagc gggttcgagg agccgttggg gttgaggatc tcggtcgcca
     2101 gcatctcggg ccaccagtcc ttgttggtgc ggccgagcag cgcgcggacg ccccccggct
     2161 ggtggatcgg gcagccgccg gcgggatcga tgggggtcgg gtcgttcatg cagtctctcc
     2221 tcgtttatct gttatggaat gccggatcgt ggcgccgcgc aatgacgggc gcatgacgcg
     2281 agcgacgggg gtcgctggcg gacggccggc atgggcgggc gtcgagtcga tggcgccatc
     2341 ctttcgttct gaccggggaa ggataggccg cgcttcccca tcgccataac cgaataagct
     2401 gaattcagtc atcgaatgaa ccgatcaagc gcgccagagt tgcggggccc gacgggacct
     2461 gccccgcccc agccacgccc cggcgacaag ggggaacatc tggcgcgggc gggggttgat
     2521 ccgatgtcgc ggcatgatgg catggcgcgg caaaggagat ggaaagtggc taacaacccc
     2581 aaccagcaga atgagaaaaa accgcaagct gaccgcgacg aagaacagcg ccgccagcag
     2641 caacagcagg gcggccagga tcgccagcag ggcggtcagc ggtccgatca ggaccggaac
     2701 aagaacccgc agcagcgctg acgaccagcc tgcatgagaa agggcggccc ccccaatatc
     2761 ttttggacgg gccgcccttt tcttttgccg gtaagggacg atcagaagct ggccttgatc
     2821 gttccgcccc aggttcgcgg atcgcccggc aggccggtga tcagcccggt gttacccggc
     2881 ccgacgctca gttgctcgag gtaattctgg tcgaacgcgt tgcgcaccca gccgaagatg
     2941 tcgagcccgc tctcggtccg gaaaccggcg cggaagttcg aaagcgaagt gccctcgatc
     3001 caggtgtagg ccgacggaga ggggttcgac gaccagtccg aacgatagct gccgtcatag
     3061 ccgaggtaga attgcccatc ctgcccgaac aggctggatt cggcgttggc ctccgccccg
     3121 aaggaaaagg cccatttcga cacgccgggc aggcgctgcc ccgaaatgtc gcagttcggc
     3181 gcgctggcgg tcccgcccga cagttcgggc gggcacggcg cgtcgacgaa cctttcatat
     3241 ttggcatcgg tataggcgcc attggcataa gcgcggaaac gctcgctcgg ccgcaccgag
     3301 aaatcgaatt cgacgccttg ggtgcggacc ttgcccgcat tggccagata gccgcggagc
     3361 acgccgaact ggccattgtt cacattagcc tggaagttct tgatgtcggt gcggaacgcg
     3421 ctgagattga aggtcgcgcg ccgatcccag aattcggctt tcacccccgc ctcgtaatga
     3481 tcgaccgatt cgggtttcac cgtcgcggcg gcgaggatcg ggtcgccgtc ggcaccgttc
     3541 ggcacaccat tctgattgat gccgcccgat ttgaacgtct tcgcataggt cgcatagaag
     3601 agcagatcgc gcgccgcctt ccacgtcgcc gtcaggtcat aagtgaaatt ccagtcgctg
     3661 aacgacggcg catattcctg cggcgtgaac acgccgagcc gggcggccga taccgcatcg
     3721 gtcaggtccg ccgtcaccgg cgaaccgtcg ccggcaaaaa cgaggcgctg gtagtagccg
     3781 tccttcttgt cataattcac ccggacgccg ggctggatcg tcagctggtc ggtgaccttc
     3841 cagctcagct gcccgaacag cgccgcactg gtgttcttga gatattgcgt gttgatcgcg
     3901 gtcaggccgt cgagcaccga gggatcgttc gacagggggt tcgacggatt gatcgtccag
     3961 cggctcgacg cggggccatg gccttcggtg ccctgcgtgt cgatacgctg gtaataatag
     4021 aagccgccga tgacgaaatc gaagcgatcg ccggtgtaat tatagcgcag ttcctgggta
     4081 tattgatcct gctgcgacgg gttctgcgac ctggtgacga tcggcaggcc ggtgaagtcg
     4141 cggtcgtttt cgggctgcca gtcccaatag cgccacgcgg tcaccgaggt cagcgtgccc
     4201 ggtccgacgt cccatttgac gcgcagcgac gcaccgccga tctcgttgcc cgcattcagg
     4261 ctggcatcga ggtcggtgag ccggtcatag ggattatcgc tgggaaaggc atagccctgc
     4321 gccgcggcca gcgcggcgaa ctggcggttc ggcggtcgct gcgtttcgcc ggtgcggacg
     4381 aagaccgagc cgcagcacac cgcctcctgc ttgttccagt cgccggcgag cgtgatgtcg
     4441 agattttcgg ccggccgcca gagcaattgg ccgcggatgc cgatattgtc ctggctctgg
     4501 atatagcggt cggtcgtgac attgtagatc gtgccgcggc ggctggtcga cgagaccgcg
     4561 atgcgcgccg cgagctggtc ggacagcggc cccgagatcg cggccttggc ctgtttgaaa
     4621 tcgagattgc cgatgctgac ctcggccttg ccctcgaatt cgaaggtcgg ctggttggtc
     4681 gtgatgttga tagcgccggc ggtcgtattc ttgccgtaga gcgtgccttg cggcccgcgc
     4741 agcacttcga tctgcgcgac atcgaggaag tcgaaggtcg aagaggcgac gcgcgaataa
     4801 tagacgtcgt cgacatagat gccgacgccc tgttcgatgc cgtcgttggt caggccgaac
     4861 ggcgccccga tgccgcggat gttgaccgcg gtgttgcgcg ggttcgacga gtagaattgc
     4921 agcgtcgggg tgagctgggt caggcggccg acgttgaagc tgcccgtatt gtcgatctgt
     4981 tcgccgtcaa cgaccgagat ggcgatgggc acgtcctgcg cggtttcctg acggcggcgc
     5041 gcggtgacga tgatctcgcc gccgtagctt ccgccctcga cctgcgcggc ggcgtcatcg
     5101 gcagcggcgg tattatcggc ggtttccgcg gcggcggcgg gcgatgcggc gaatgccaga
     5161 gccaaggaaa gcgtgaagag gctggccgga acgcgccggc ggacagacgg gtattttgcg
     5221 gtcatgacaa tttcctgttg ctttgaagat gcaagaaaat tgccacatcc ggtggtccgg
     5281 tctgcggcat taagggggga gtttcctccc gttgggagtc gctgaaaatc agttggggta
     5341 gcggtgccgt tcggtcacat cgacctgcac cagacgtcct tcgtgaacgg tgagctggat
     5401 cgcgccgaac ttcagcttgt cgagtgcgtc gagcacggtc tggatggcgc gcggcacctc
     5461 ttcggagccg cctttggcga cttcgttgat agcgctcata atcattcctt tccctcttgg
     5521 atttgcccca caagcatatg ccaagtgatt tagttgacaa tgattgattt gctttctgcg
     5581 ccgaaaggtt cggttgtcgc gcccaatgga ttcacgcgtg cggcgggtcg acgggcgcaa
     5641 tgatgccaag ggatgacgcc aagggatgac gccagggatt gcgcgcgagt cccagtgctg
     5701 gcaaaggcag ccttaatgag caaaattaac gatctgatgg cccgcgggac cgagcttctc
     5761 ggcagcgact atgccatcct ctgcggcgcg atgagctggg tttccgaacg tcatctggtc
     5821 agcgcgatca gcaatgccgg cggctttggc gtgatcgcgt gcggcgcgat gacgcccgcg
     5881 ctgctcgatg ccgaaatcgc ggggaccaag gcgctggcga cgcgcaattt cggcgtcaac
     5941 ctgatcacca tgcacccgca attgttcgaa ctgatcgagg tgtgcgccaa gcatcgcgtc
     6001 ggccatgtcg tgctcgcggg cggcctgccg cccaaaggca gtctggaggc gatcaaggcg
     6061 tcgggcgcca aggtcatctg tttcgcgccg acgctggcgc tcgcgaaaaa gctcgtccgt
     6121 tcgggcgtcg acgcgctggt gatcgagggg atggaggcgg gcggccatat cggcccggtt
     6181 tcgaccagcg tgctcgcgca ggagattctg ccgacgctcg ccgacgaagt tccggttttc
     6241 gtcgccggcg gcatcgggcg cggcgaagcg atcgccgcct atctggagat gggcgcgtcg
     6301 ggggttcagc tcggcacgcg cttcgtctgc gcgaccgaga gcatcgcgca tcccaatttc
     6361 aagaaggcgt tcatgcgcgc ctcggcacgc gaggcggttg cgagcgtcca gatcgacccg
     6421 cgcctgccgg tcatcccggt ccgcgcgctc aagaatgccg ggaccgaagc ctttactgtg
     6481 aagcagcgcg aagtcgcgaa caagctcgac aagggcgagg tcgacatgat ggaagcgcag
     6541 ctcgaaatcg agcattattg ggcgggcgcg ctgcgccgcg ccgtgatcga cggcgacgtc
     6601 gaaaatggtt cgttaatggc agggcaatct gtgggtatgg tatccgaaga ggagagcgcg
     6661 gcagcaattg tcgcatctct ggtgcaacag gccgaagccg cgttgcacgc tcggggttga
     6721 tgccgcatgg tgatgcggtt ggggagtggg tcatgaattt gtcgcgtaag atcatcgtag
     6781 ccggcctgct ggcaagcgta gccgcctgtg cgccgaaacc gcccccgccg cccccgccgc
     6841 cccctccccc gccggtcgtc gtcaccatcc cgccgcggcc gctgccgccg ggcaatgcgg
     6901 cgctgacgca gattctcccc ggccgcggga tcgacggacg cttcgtcacc gcgaacagcg
     6961 gcgtgaccgg cgaccgcgcc ttctggcagc tcaagatcgg gctgaacgtc gccgccatcg
     7021 gttgccgcgg catcgaggaa acgacgctgg tttccgccta taacaatatc atcaagacgc
     7081 acggcaaggt gatcaaatcg accgaaaaat cggtgatcaa ggagcttggc aaggaaaaca
     7141 agaataacgg catcgcgccg cgcgaccgcc tgtcgaccca gctgttcaac tattttgcgc
     7201 agccgcccgc gcagcgcgct ttctgtccgc gcgccaatga aatcgcgcag ctcgtttcga
     7261 cgacgccgac cgatcagctc gtcgaacagg cgcccgcgca cctcgcgcgg ctcgacgagc
     7321 cgttcaagca attttacgag gcctatgccc aatatcagtc cgacgtgatc gcctgggacg
     7381 cgaaatatgc gccgccgccg gcgatcatga cgacgccggt cccggcgacg cccatcggcc
     7441 ccgccatcac gaccggcacc acgccggtcg cgccgacgac ggtcccggcc agcccgaccg
     7501 gaaatccctg agggaattgc gggacattca ggaatagaat gggcaatcgc acgcgggcta
     7561 ttggcgcgcg ccgcatcctt ctgttagcag gattgcgatg accaacgccc cgcccccgcc
     7621 ctcgtcggcc gcccagtcgg cgcgcaccat attgacccgg ctgcacgagg tcatggcgtc
     7681 gcgcgtcaat gcgcagggca agctcaatca ggtcgtcggc atcatcggcg agtgcctcga
     7741 tagcgaggtc tgttcgatct atctgctgcg cgacggcacg ctcgaacttt atgcgacgcg
     7801 cgggctgaag caggaggcgg tgcacgtcac gcggcttggc ctcggcgaag ggctggtcgg
     7861 catgatcgcc gaccagatcg agacgctgaa cctcgacgaa gcggcggcgc accccgactt
     7921 ttcctatcgt cccgaaaccg gcgaggaatt gttccacagc tttgccggcg tgccgatcat
     7981 ccgccgcgag cgcgccgtgg gggtgctgtg cgtccagcat gccgacccgc ggcgttacga
     8041 cgatatcgag atcgagacgc tgcagaccgt cgcgatggtg ctgtccgagc tgatcgccaa
     8101 cgcggacctg gtcgataccg ccgcgcgcac cgacgccgcc gccgccgacc agtcggcgca
     8161 gcggctgaac ggccagaagc tggtcgacgg catgggcgcc ggggtcgcgg tgttccacca
     8221 gccgcgcatc acgatcgaac atacggtcgc cgacgatacc gaggccgagc gccaccgcgt
     8281 ctatgccgcg ttcgacaaaa tgcgcgagca gatcgaccgc atggcgagtt cggccgactt
     8341 cggcgtcggc ggcgagcatg aggaggtcat cgagacctac aagatgttcg cctatgacga
     8401 gggatggtcg cggcggatca acgaagcgat cgacagcggg ctgaccgccg aagccgcgat
     8461 cgaacgcgtc cagcagcgca cccggatgcg gatgcgccag atcgacgatc cgctgctgcg
     8521 cgaccgcatg cacgacctcg aggatctgtc gaaccggctg atccgcatcg tgtcggggca
     8581 gatgggcacg gccgcgcaga tgggcctgcg gcacgattcg atcctgatcg cgcgcaacct
     8641 cggccccgcc gagctgctcg aatatgaccg ccgccgcctg aaaggcgtcg tgctcgagga
     8701 aggatcgctc accgcccacg tcatcatcgt cgcgcgcgcg atgggggtgc cggtgatcgg
     8761 ccgcgtcagc gatgtgcgca gctcgatccg cgaaggcgac ctgctgctgc tcgacgcgac
     8821 ggcggggcag ctccatgtcc gtcccacgca ggcggtgcag gatgccttcg acgccaagct
     8881 cgagatttcg cagaagcgcc gcgccaacct cgcatcgctg cgcgacatgc ccgcggtcac
     8941 caaggacggg gtgccgatcg aactgatgat caacgcgggg ctgcgcgaag atgtcgccgc
     9001 gctcgacctg acgggcgcgc gcgggatcgg gctgttccgc accgaattcc agttcctcgt
     9061 atcggcgacg ctgccctcgc gcgaccgcca gcaacggctg tatcgcgacg ttctcgatgc
     9121 cgcgggcgac cggccggtga tctttcgcac cgtcgacatc ggcggcgaca aggcgctgcc
     9181 ctatatgaat gtcgatggca ccgcgcagga ggaaaatccg gcgatgggct ggcgcgcgct
     9241 gcggctggcg ctcgaacgcg aagggctgct caaggttcag gcgcgcgcgc tgatggaggc
     9301 ggccgccggc cgcacgctcc acgtcatgtt tccgatggtg tccgagccgt gggaatatga
     9361 agcggcgcgc aacctgttcg tcggccagcg cgcgtggctc gcgagccaca acaagaagct
     9421 gcccgtcgcg atccgctatg gtgcgatgct cgaggttccg ggtcttctcg aaacgctcga
     9481 cctgatgctg ccgcacctcg acttcctgtc ggtcggcacc aacgacctca cccagttcct
     9541 cttcgccgcc gaccgcgcgc acccgcggct cgccgaacgt tacgactggc tctctcccgt
     9601 cgtgatgcgt tacctcgcgc gcatcgtccg caccgtgtcg ggatcgaagg tcgcgctggg
     9661 cgtgtgcggc gagatgggcg gacggcccct cgaagccatg gcgctgctcg gcgcgggcat
     9721 cgagcggctg tcgatcaccc ccgccggcgt cggcccggtc aag
//
LOCUS       DATMSU010000459         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_178396,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000459 DATMSU010000000
VERSION     DATMSU010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178396"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VN034_12275"
     CDS             <1..237
                     /locus_tag="VN034_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWV61429.1"
                     /translation="MRSHGGLTLAEAMRAAAAPMLIQRAVVAGDGEGGLMATGQVAGR
                     LTDLPSVADLLSRIESDAQACLAALAPSLQRIAV"
     gene            237..986
                     /locus_tag="VN034_12280"
     CDS             237..986
                     /locus_tag="VN034_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HWV61430.1"
                     /translation="MPIRTNIADRIGEIILDHPPVNALDSEGWNELPGHVERLGRDPE
                     VRCVLVRAEGRGFCGGVDIKEMQAHPERIVELNRGNYLTFRAIRGCEVPVVTAVHNFV
                     IGGGIGICGASDVVIAADNAYFSLPEVDRGAMGGASHLQRMFPLQKVRAAFFTGGRIP
                     AAEAYRLGAVEAVHPADRLAEEARAFAAIIASKSRRALVLAKEALNGIEPRDVDHGYR
                     FEQGFTLEMYMHEDSQRSRDEFVATGNSAKF"
     gene            989..2161
                     /locus_tag="VN034_12285"
     CDS             989..2161
                     /locus_tag="VN034_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61431.1"
                     /translation="MDLQYTPEEQAFRAEVRQWMAANVPTTPLPDFDSTRAGFEAHRE
                     WERTLHKGGWGMVTWPAEYGGRGLDLIRWLIFEEEYYRAGAPGRVNQNGIFLLGPTLI
                     EYGTPEQKARFLPAMAAGDDIWAQAWSEPGAGSDLAGVRATATRVKNAEGGDDYILSG
                     HKIWSSRAVHADWAFGLFREPGSERHKGLSLILFPLNAPGVTVKPIQRLDGKPVFAEL
                     FLDDVRIPIANRLGDEGQGWAVCMSTAGFERGLMLRSPARFQVAAAKLVELWKERGSD
                     PLLGAEVMQAWMDADAYALSIYATASRLMAGGAIGPETSTNKIFWSELDLDLHKTALS
                     LLGAEAELTAALPGAAAPVADWLDGYVFSLAGPIYAGANEIQRNIIAERMLGMPRE"
     gene            2161..3195
                     /locus_tag="VN034_12290"
     CDS             2161..3195
                     /locus_tag="VN034_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV61432.1"
                     /translation="MDFRLTDEQEMLAGEVGKLLAATCTGADLRRLLESGEAIDRGRW
                     GQMLVMGLPGVMGPEEAGGAGLGAIEMALIATACGYAALPEPLVDHAGVAVPMLVAAG
                     ADLEKALFGSRVAVGHPVNPLVADADSAAALLLADGDDVHLVARDQVTLTRHDSIDPF
                     RRLFAVQWSPMPATRIGGAALWEQALDHGALFAAAQLLGLAQRAVDMAVAYTKEREQF
                     GKPIGSFQAVKHHLANAQVKIEFARPVILAAAAMPHASARISHAKLAATAAADAATHA
                     AVQVHGAMGFSWEVDVHFLLKRSLALGQAWGTPAFHRARVADHIRSAPLGADRTFDTP
                     VSEEVRREAA"
BASE COUNT          521 a         1038 c         1121 g          547 t
ORIGIN      
        1 atgcgcagcc acggcgggct gacgcttgcc gaagcgatgc gcgccgcggc tgctccgatg
       61 ctgatccagc gtgcggtggt cgctggcgac ggcgaaggcg ggctgatggc gaccggccag
      121 gttgccgggc gcctcaccga cctgccgagc gtcgcagatc tgctgagccg gatcgaaagc
      181 gatgcacagg cgtgcctcgc cgcgcttgcg ccaagccttc aaaggatagc agtctgatgc
      241 cgatacgcac gaacatcgcc gaccggatcg gcgagatcat actcgatcac cccccggtca
      301 acgcgctcga tagcgagggc tggaacgaac tgcccgggca tgtcgagcgc ctcggccgcg
      361 accccgaggt gcgctgcgtg cttgtccggg ccgaggggcg cggtttttgc ggcggggtcg
      421 atataaagga gatgcaggcg catcccgaac gcatcgtcga actcaatcgc gggaattacc
      481 tgaccttccg cgccattcgc ggctgcgaag tgcccgtggt gaccgcggtt cataatttcg
      541 tgatcggggg cgggatcggc atctgcggcg cgtccgacgt cgtgatcgcc gccgacaatg
      601 cctatttctc gctgcccgaa gtcgatcgcg gcgcaatggg gggcgcgagc catttgcagc
      661 gcatgttccc cctgcaaaag gtgcgcgcgg cctttttcac cgggggacgc attcccgctg
      721 cagaagccta tcgattgggg gctgtcgagg cggtccatcc agccgaccgg ctcgccgagg
      781 aagcgcgcgc ctttgccgcg atcattgcat cgaagagccg tcgcgcgctg gtgctcgcca
      841 aggaggcatt gaacggcatc gagccgcgcg acgtcgacca tggctaccgc ttcgaacagg
      901 gcttcacgct cgaaatgtac atgcacgaag acagccagcg gtcgcgcgac gaatttgtcg
      961 ccacgggcaa ctcggcgaaa ttctgatcat ggatttgcaa tacacccccg aagaacaggc
     1021 ctttcgcgcc gaggtccgcc agtggatggc cgcgaatgtg ccgaccacgc cgctgcccga
     1081 tttcgattcg acgcgcgcgg ggttcgaggc gcaccgcgaa tgggagcgta cgctccacaa
     1141 agggggctgg ggcatggtca cctggccggc cgaatatggc gggcgcgggc tcgacctgat
     1201 ccgctggctg atcttcgaag aggaatatta tcgcgccggg gcgccggggc gggtgaacca
     1261 gaacggcatc ttcctgctcg gtccgacctt gatcgaatat ggcacgcccg agcaaaaggc
     1321 gcgcttcctt cccgcgatgg cggcgggcga cgatatctgg gcgcaggcgt ggtccgaacc
     1381 cggcgcgggc agcgaccttg cgggcgtccg tgccaccgcg acgcgcgtca aaaatgcaga
     1441 ggggggcgat gactatatcc tctcgggcca caagatctgg tcgtcgcgcg cggttcacgc
     1501 cgattgggcg ttcggcctgt tccgcgagcc cgggagcgag cgccacaagg gactgtcgct
     1561 gatcctcttt ccgctgaatg cgcccggggt aacggtgaag ccgatccagc ggctcgacgg
     1621 caagccggtc tttgccgaac tcttcctcga cgatgtccgc atccctattg ccaaccggct
     1681 cggtgacgag ggtcaggggt gggcggtgtg tatgtcgacc gcggggttcg agcgcggatt
     1741 gatgctgcgc agtcccgcac gctttcaggt cgccgctgcg aagcttgtcg agctttggaa
     1801 agagcgcggc tccgatccgc tgcttggagc cgaagtgatg caggcgtgga tggacgccga
     1861 tgcttatgcg ctgtcgatct atgcaaccgc gagccggctg atggcgggcg gcgcgatcgg
     1921 gcccgagacg agcacgaaca agatattctg gtccgaactc gacctcgatc ttcacaagac
     1981 cgcgctttcg ctgctcggcg ccgaggccga actcaccgcc gccctgccgg gcgcggctgc
     2041 acccgtcgcc gattggctcg acggatatgt cttctcgctc gcggggccga tctatgccgg
     2101 cgcgaacgag atccagcgca acatcatcgc cgagcggatg ctcggcatgc cgagggaatg
     2161 atggatttcc gcctgaccga cgaacaggag atgctggcgg gcgaggtcgg caagctgctg
     2221 gccgcgacct gcaccggcgc cgaccttcgc cggctgctcg aaagcggcga ggcgatcgat
     2281 cgcggacgct gggggcagat gctcgtgatg ggcctgcccg gggtgatggg gcctgaggaa
     2341 gcgggcggcg ccgggctcgg tgcgatcgag atggcgttga tcgcgacggc gtgcggttat
     2401 gccgcgctgc ccgaaccgct ggtcgatcac gcgggggttg cggtgccgat gctcgtcgcc
     2461 gcgggcgccg accttgaaaa ggcgctgttt ggctcgcggg tcgcggtcgg gcatccggtc
     2521 aatcccttgg tcgctgacgc tgatagcgcg gctgccctgc tgcttgccga tggcgatgat
     2581 gtccatctcg tcgcgcgcga tcaggtgacg ctgacccggc acgacagcat cgatcccttc
     2641 cggcggctgt tcgcggtcca atggtcgcca atgcccgcga cgcgcatcgg cggggcggcg
     2701 ctgtgggaac aggcgctcga tcatggcgcg ctcttcgccg ccgcgcagct cctcggcctc
     2761 gcccagcgcg cggtcgatat ggcggtggcc tatacgaagg agcgcgaaca attcggcaag
     2821 ccgatcggat ccttccaggc ggtgaagcat caccttgcca atgcgcaggt gaagatcgaa
     2881 tttgcgcggc cagtgatcct cgccgccgct gcgatgccgc acgcctcggc acggatcagt
     2941 cacgccaagc tcgccgcgac cgccgccgcc gatgccgcga cgcacgccgc ggtgcaggtg
     3001 catggcgcga tgggtttcag ctgggaagtc gacgtccatt tcctgctcaa gcgcagcctc
     3061 gcgctcgggc aggcttgggg cacgcccgcc ttccaccgcg cgcgcgtagc cgatcatatc
     3121 cggtcggctc cgctcggggc cgatcgcacc ttcgatacgc cagtcagcga ggaggtgcgc
     3181 cgtgaagccg cttgaggtcg cccgcagctt gcgcgccgat cccgagg
//
LOCUS       DATMSU010000460         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_16853, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000460 DATMSU010000000
VERSION     DATMSU010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16853"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VN034_12295"
     CDS             complement(<1..228)
                     /locus_tag="VN034_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61433.1"
                     /translation="MTSRRGGERLIRLLLLALAGALAGMLLGEESAEPLAAARDGGDP
                     ASYAQYSANPDARVAQGETAAPCLGCPDSYGV"
     gene            493..858
                     /locus_tag="VN034_12300"
     CDS             493..858
                     /locus_tag="VN034_12300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HWV61434.1"
                     /translation="MAKFSLMLLAVPLAMAGFSLPAAANPTVEDNGDRHTAIVRYDDL
                     NLSSVEGRKRLTTRVKYAVRKVCGARPGDHKGLRERLSANRCQDTALADADVKLAALF
                     NGDGARFADRGRIVILSAP"
     gene            complement(1231..2817)
                     /gene="guaA"
                     /locus_tag="VN034_12305"
     CDS             complement(1231..2817)
                     /gene="guaA"
                     /locus_tag="VN034_12305"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWV61435.1"
                     /translation="MPTPDSSSPPESILIIDFGSQVTQLIARRVREAGVYSEIVPFSA
                     AEAALERMKPKGVILSGSPASVPASGSPRAPQSVFDSGLPILGICYGQQVMTQQLGGQ
                     VEPGGADGERDGEFGRAFLTVTEPCVLFDGLWEVGERHQVWMSHGDRVTAFAPGFRIV
                     AISDGAPFALIADDERRYYGTQFHPEVVHTPDGAKLIANFVRHVCGCSGDWTMAEFRA
                     TKIAEIRAQVGDQRVLCALSGGVDSAVAAVLIHEAIGEQLTCVFVDHGLLRLNERHQV
                     ETLFRDHYNIPLVVVDAEERFMAGLAGVTDPEAKRKFIGKAFIDIFEEEAKKIGGADF
                     LAQGTLYPDVIESVSFTGGPSVTIKSHHNVGGLPERMNMKLVEPLRELFKDEVRLLGK
                     ELGLPDIFVGRHPFPGPGLAIRIPGEVSKDRCDILRKADAVYLEEIRNAGLYDAIWQA
                     FAVLLPVKTVGVMGDGRTYDHVCALRAVTSTDGMTADIYPFDASFLSRCATRIINEVQ
                     GINRVVYDYTSKPPGTIEWE"
BASE COUNT          548 a          982 c         1041 g          547 t
ORIGIN      
        1 gacaccatag ctgtcggggc aaccgaggca cggggcggcg gtttcgccct gggccacgcg
       61 cgcgtcggga ttggcgctgt actgcgcata ggaagcggga tcgccgccgt cacgcgctgc
      121 cgcgagcggc tcggcgctct cctcgcccag cagcatcccc gcgagcgctc cggccagcgc
      181 gagcaacagc aggcggatca gccgttcgcc gccgcgccga ctcgtcacga aagataattt
      241 cctcgccacc gggggatttg gccacgggcg cgcgttcgat gcaagcccgg cgccatgaaa
      301 ttgcccctcc gctggccatt cgtcgacgga tggacgcact tcgtccgtaa atcgagcggc
      361 cgttcgcccc gatcgatcca aagatgaatc gcgtgacgtc ccgggagtat cacctgtgtt
      421 gcgggatatc ggaagacggt ctccccggtc cgcgatccgg tatcccgcaa cccttgcaga
      481 gaggactccc ccatggcgaa attttccctg atgctgctgg cagtgccgct ggcgatggcc
      541 ggcttttccc ttcccgcagc cgcaaacccc acggtcgagg acaatggcga tcggcatacc
      601 gcgatcgtcc gatatgacga tctgaacctt tcgagcgtcg agggacgcaa acgcctgacc
      661 acccgcgtca aatatgcggt ccgcaaggtt tgcggcgctc ggccgggtga tcataagggc
      721 ctgcgcgagc gcctgtccgc caatcgctgc caagatacgg ccttggccga tgcggatgtg
      781 aagctggcgg cgctgttcaa cggcgatggc gcccgtttcg ccgaccgcgg ccggatcgtc
      841 atcctctcgg ctccctgacc atcttcagcc cctcccccca agggggaggg gttcaagctt
      901 tgcagacaag atatggcggt atggctgcct ccgcgccaaa ctagccgtgg cccccgcgaa
      961 ggcgagggcg ctgtcggttt acgcagggcc atagagtagg ccgctagcgg gcccggcctt
     1021 cgtgggggcg acggattagg gagcgtccgc tcacacacca tttcggccgt tcgccgccga
     1081 cttgcgacaa tgaccggcaa cggccgaaag ccgtcagtac gagcagggca tcaagcggca
     1141 tattttccgg cagtgactgc cgaaagctat cattttatca gttgtttagg gtaataacac
     1201 cgtcagggga ggccgctctg cccgattttt tcattcccac tcgatcgttc ccggcggctt
     1261 cgacgtatag tcgtagacca cgcggttgat gccttgcacc tcgttgatga tccgcgtcgc
     1321 gcagcggctg aggaagctcg cgtcgaaggg gtagatgtcg gcggtcatcc cgtcggtcga
     1381 ggtgacggcg cggagcgcgc agacatggtc gtaggtgcgc ccgtcgccca tcacgcccac
     1441 cgtcttgacg ggaagcagca ccgcgaacgc ctgccagatc gcgtcgtaaa ggccggcatt
     1501 gcggatttcc tccagataga ccgcgtccgc cttgcggagg atatcgcagc ggtccttcga
     1561 cacttcgccg gggatgcgga tcgcgagacc cgggccgggg aagggatggc ggccgacgaa
     1621 gatgtcggga aggccgagtt ccttgccgag cagccggacc tcgtccttga agagttcgcg
     1681 caagggttcg acgagcttca tgttcatccg ctcgggcagg ccgccgacat tgtggtggct
     1741 cttgatcgtc acgctcgggc cgccggtaaa gctgaccgat tcgatcacgt cgggatagag
     1801 cgttccctgc gcgaggaagt cggcgccgcc gatcttcttc gcttcttcct cgaagatgtc
     1861 gatgaaggct ttgccgatga acttgcgctt tgcctcgggg tcggtgacgc cggcaaggcc
     1921 ggccataaag cgttcttcgg catcgacgac gacgagcggg atgttgtaat ggtcgcggaa
     1981 cagtgtctcg acctggtggc gctcgttgag gcggagcaag ccgtggtcga cgaagacgca
     2041 ggtgagctgc tcgccgatcg cctcgtggat cagtaccgcg gcgaccgcac tgtcaacccc
     2101 gcccgagagt gcgcacagga cgcgctggtc accgacctgc gcgcggattt cggcgatctt
     2161 ggtcgcgcgg aactcggcca tcgtccaatc cccgctgcag ccgcagacat ggcggacgaa
     2221 attggcgatc agcttggcgc cgtcgggggt gtggacgacc tcggggtgga actgcgtgcc
     2281 gtaatagcgg cgttcgtcgt cggcgatcag cgcgaagggc gcgccgtcgc tgatcgcgac
     2341 gatgcgaaaa ccgggggcga aagcggtgac gcggtcgccg tggctcatcc acacctgatg
     2401 gcgttcgccg acctcccaca gtccgtcgaa gagcacgcag ggctcggtga cggtcaggaa
     2461 ggcgcggccg aattcgccgt cgcgttcgcc gtcggcgccg ccgggttcga cctgtccgcc
     2521 gagctgctgg gtcatcacct gctggccgta gcagatgccg aggatgggaa ggccgctgtc
     2581 gaacaccgat tgcggcgcgc gcgggctgcc gctcgcgggg accgatgcgg gtgagcccga
     2641 gaggatcacc cccttgggct tcatccgttc gagcgcggcc tcggcggcgc tgaacgggac
     2701 gatctcgcta taaacgcccg cttcgcggac gcggcgggcg atgagctggg tgacctgcga
     2761 cccgaagtcg atgatcagga tggattcggg cggtgaggaa gagtcgggag tcggcatggc
     2821 tggccgataa gggcatgggg ccatgctgtc cagcggtcga tatgggacac cggtcgaaca
     2881 agcatggccc cgacacattg ttgcgacaat tgcgcgacat aagtgaacgc tggacaaatt
     2941 acggtgtcag gaggcgttaa gccccgtcat gccaagaaaa aaccaagaca gaaaagaaca
     3001 aaggctctcc cccatgaata atatccgcct gcctatttcc gcacgcgccg cgctcctcgc
     3061 ggccggggct tcgttcgcgc tgccggcgat ggcagcggcg ccgatcggac ccgccgcg
//
LOCUS       DATMSU010000461         4673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_46155, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000461 DATMSU010000000
VERSION     DATMSU010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46155"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VN034_12310"
     CDS             <1..235
                     /locus_tag="VN034_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HWV61436.1"
                     /translation="GNEDGAHHVSAQIFQALNDVGWTIPAVAACYWVGEAMGSTDFKD
                     LEHRPRKVTETAKMVAANAAHLARLLKQAPYPG"
     gene            292..1602
                     /locus_tag="VN034_12315"
     CDS             292..1602
                     /locus_tag="VN034_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWV61437.1"
                     /translation="MKSLPLAALVALGLALAPSVQAKLSKAESGMAKTVAAEQDRSLA
                     LLEKLVNQNSGSLNLEGVEKVGQMMRAELEPLGFKVEWKPMRETGRAGHLIATHVGKP
                     DAKRLLLIAHLDTVFEPDSPFQTFTRKGDKGEGPGAGDDKGGMVVIVAALRAMQAAGT
                     LKDANIEIHMTGDEEDAGSPIEKARADLIAAGKRSDVALDFEGLVRDNGADMGSVARR
                     SSDSWTVTATGKSAHSSGIFSAAAGDGAIYELTRIIHRFRTELPEPNLTFNVGLIAGG
                     QQADLDAGGIRATVSGKTNIIAPIAVARGDLRALSPEQIERVKAKMTAIVADHAPGTD
                     AKIAFDPGGYPSMAPTDGNRALLAKLNGVNRDLGLPEMAPLDPLKRGAGDISFVAADV
                     DGLAGLGPYSTGDHAPGEAVDIPSISRQATRAAILMSRLSAEKR"
     gene            1698..3131
                     /locus_tag="VN034_12320"
     CDS             1698..3131
                     /locus_tag="VN034_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV61438.1"
                     /translation="MSTETHLKQHYIGGRWVDSKGGKLHDVINPATEEAASTVVLGSA
                     ADVDDAVAAAKEAFKSFSQTTREERLDLLNRIVEEYKKRAPDLAKSMASEMGAPVSFA
                     GTAQVGAGIGGFLGTIAALKDFSFTEKYAAGMIAYEPIGVVGMITPWNWPLNQIALKV
                     APALAAGNTMVLKPSEECPGNAVIFAEILEAAGVPPGVFNLVQGDGPTVGNAISAHPG
                     IEMVSFTGSTRAGILVAKAAADTVKRVHQELGGKSPNIVLPDADFAAVLPPTVQGVLV
                     NTGQSCIAPTRILVQKEREAEAVGVIKAMFDGTSVGDPQAEGGHIGPVVNKAQFDKIQ
                     GLIQSAIDEGATLETGGTGLPANVNRGYYIKPTVFSGVTPDMRIAKEEVFGPVATIMA
                     YDDLDQAVDIANDTEYGLSAVVSGDPAKAAEIAPKLRAGMVAVNAWGPGPGAAFGGYK
                     ASGNGREGGVFGLKDFMEVKSISGIPA"
     gene            3303..4529
                     /gene="argJ"
                     /locus_tag="VN034_12325"
     CDS             3303..4529
                     /gene="argJ"
                     /locus_tag="VN034_12325"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWV61439.1"
                     /translation="MTTRSPLAPAAFPALPDIAGVTLRVARARYKNWDRCDLTFVELA
                     PGTTVAGVFTRNVCCSSEVELGREQVKGGTGRALIVNAGNSNAFTGYRGREAVEQIMA
                     QVAGHLGCDAGEVFVSSTGVIGVPLPKDKARTGVEAALTAELCSWEAAAETIGTTDTF
                     AKGSTASAIVGGKTVHVAGIVKGSGMIAPDMATMLGYIFTDAAVAPALLQEMLSEATG
                     GSFNSITVDSDTSTSDTVLLFATGQAGNTPLTTRDDPGADALYAAIRTVALDLAQQVV
                     RDGEGASKFIEVQITGAVSDDSAKRVALAIANSPLVKTAIAGEDANWGRVVMAVGKAG
                     EPADRDRLAIRFGDHWVAKDGLPVDGYEEAPVAAHLKGQDIRVGADLGLGEGRATVWT
                     CDLTHGYISINADYRS"
BASE COUNT          848 a         1634 c         1531 g          660 t
ORIGIN      
        1 cggcaacgag gacggcgcgc accatgtctc ggcgcaaatc tttcaggcgc tgaacgatgt
       61 cggctggacg attcccgcgg tcgccgcctg ctattgggtc ggcgaggcga tgggatcgac
      121 cgatttcaag gacctcgagc accgcccgcg caaggtcacc gaaaccgcga agatggtcgc
      181 cgccaacgcg gcgcacctcg cacgactgct caaacaggcg ccctaccccg gctgagcgct
      241 tgccgaggcg tggtggaggc ggcacaagag ggtatccaga ggagcctccc catgaaatca
      301 ctgccgcttg ccgctctcgt cgcccttggg cttgcgctcg cgccctccgt gcaagcgaag
      361 ctgtcgaaag ccgaaagcgg catggcaaag accgtcgcgg ccgagcaaga ccgctcgctc
      421 gccttgctcg aaaagctggt caaccagaac agcggctcgc tcaacctcga aggggtcgag
      481 aaggtcgggc aaatgatgcg cgccgagctc gaacccctcg gcttcaaggt cgaatggaag
      541 ccgatgcgcg agaccggccg cgccggccat ctgatcgcga cgcacgtcgg caagcccgat
      601 gccaagcgcc tgctcctgat cgcgcatctc gacaccgtct tcgaacccga ctcgcccttc
      661 cagacattca cccgcaaggg cgacaagggc gaaggccccg gcgcgggcga cgacaagggc
      721 ggcatggtcg tcatcgtcgc cgcactgcgc gcgatgcagg cggcgggtac gctcaaagac
      781 gccaatatcg agattcacat gaccggcgac gaggaggatg cgggcagccc gatcgagaag
      841 gcgcgcgccg acctgatcgc cgcgggcaag cgcagcgacg tcgcgctcga tttcgaaggg
      901 ctcgtccgcg acaatggcgc cgacatgggc tcggtcgcgc gccgctcgtc ggacagctgg
      961 accgtcaccg cgacgggcaa gagcgcgcac agctcgggca tcttcagcgc cgcggcgggc
     1021 gacggcgcga tctatgagct gacgcgcatc atccaccgct tccgcaccga gcttcccgaa
     1081 ccgaacctca ccttcaacgt cgggctgatc gcgggcgggc agcaggccga tctcgacgcg
     1141 ggcggcattc gcgccaccgt cagcggcaag accaacatca tcgccccgat cgcggtcgcg
     1201 cgcggcgatt tgcgcgcgct gtcgcccgag cagatcgaac gggtgaaggc caagatgacg
     1261 gcgatcgtcg ccgaccacgc cccgggcacc gacgccaaga tcgccttcga ccccggcggc
     1321 tacccctcga tggcgccgac cgacggcaac cgggcgctgc tcgcgaagct caatggcgtc
     1381 aaccgcgacc tcggcctccc cgaaatggcg ccgctcgacc cgctgaagcg cggcgcgggc
     1441 gacatcagct tcgtcgccgc cgatgtcgac gggctcgcgg gactggggcc ctactcgacc
     1501 ggcgaccatg cccccggcga agccgtggat attcccagca tcagccgcca ggcgacgcgc
     1561 gccgccatat tgatgtcgcg cctttcggcc gaaaaacgct gagccgtcag ttttcgcagt
     1621 tggccgtgct tgattcatcg ggcacagtga ttaggtagca atccgagacg gaatcgaccg
     1681 ccgatgggag acaggacatg agcaccgaaa cgcatctcaa acaacattat atcggcggcc
     1741 gctgggtcga ctcgaagggc ggcaagctcc acgacgtgat caaccccgcg accgaggaag
     1801 cggcatcgac cgtcgtgctc ggcagcgcgg ctgacgtcga cgacgcggtc gccgcggcga
     1861 aggaagcgtt caagagcttc tcgcagacga cgcgcgagga acgcctcgac cttttgaacc
     1921 gcatcgtcga ggaatataag aagcgcgcgc ccgacctcgc caagtcgatg gcgagcgaaa
     1981 tgggcgcccc cgtcagcttc gcgggcaccg cgcaggtcgg cgccggcatc ggcggcttcc
     2041 tcggcacgat cgccgcgctc aaggatttca gcttcaccga aaaatatgcc gcgggcatga
     2101 tcgcgtacga gccgatcggc gtcgtcggca tgatcacgcc gtggaactgg ccgctgaacc
     2161 agatcgcctt gaaggtcgca ccggcgctcg ccgcgggcaa tacgatggta ttgaagccct
     2221 ccgaagaatg ccccggcaat gcggtgatct tcgccgaaat cctcgaagcg gcgggcgtgc
     2281 ccccgggcgt gttcaatctg gttcagggtg acggcccgac cgtcggcaac gcgatcagcg
     2341 cgcaccccgg catcgaaatg gtgagcttca ccggctcgac ccgcgcgggc atcctcgtcg
     2401 ccaaggcggc cgccgacacg gtcaagcgcg tccatcagga actcggcggc aagtcgccga
     2461 acatcgtcct gcccgacgcc gatttcgccg cggtgctgcc gccgacggtg cagggcgtgc
     2521 tcgtcaacac cggccagagc tgcatcgcgc cgacgcgcat cctcgtccag aaggagcgcg
     2581 aggccgaagc ggtcggcgtg atcaaggcga tgttcgacgg gacttccgtg ggcgacccgc
     2641 aggccgaggg cggccatatc ggccccgtcg tcaacaaggc gcagttcgac aagatccagg
     2701 gcctgatcca gtcggcgatc gatgaaggcg cgacgctcga gaccggcggc accggcctgc
     2761 cggcgaacgt caaccgcggc tattatatca agccgacggt gttctcgggc gttacccccg
     2821 acatgcgcat cgcgaaggaa gaggtgttcg gccccgtcgc gacgatcatg gcctatgacg
     2881 atctcgacca ggcggtcgat atcgccaacg acaccgaata tggcctttcg gccgtcgtct
     2941 cgggcgaccc cgccaaggcc gccgaaatcg cgcccaagct gcgcgccggc atggtcgcgg
     3001 tcaacgcgtg gggccccggc ccgggcgcgg cgttcggcgg ctacaaggcg tcgggcaacg
     3061 gccgcgaagg cggcgtattc ggcctcaagg acttcatgga agtgaagtcg atcagcggca
     3121 ttccggcgta acttcttcta agccctctcc ttcaggggag ggcagcgaga cttgggagct
     3181 tgctccctag tcgcagcggg tgggggccag cggccttgca caaggtcgca cgccccaccc
     3241 caacccctcc cctaaagggg aggggctttt gtatgaacgc aacacccgct aagcgccctc
     3301 ccatgaccac ccgctcgccg ctagcccccg ccgccttccc cgccctcccc gacatcgccg
     3361 gggtcacgct ccgcgtcgcg cgcgcgcgct acaagaattg ggaccgctgc gacctgacct
     3421 ttgtcgaact cgcccccggc accaccgtcg cgggcgtctt cacgcgcaac gtctgctgct
     3481 cgtccgaggt agaactcggc cgcgaacagg tgaagggcgg caccggccgc gcgctgatcg
     3541 tcaacgcggg caacagcaac gccttcaccg gctatcgcgg gcgcgaggcg gtcgagcaga
     3601 tcatggcgca ggtcgcgggc cacctaggct gcgatgccgg cgaagtgttc gttagctcga
     3661 ccggcgtgat cggcgtgccc ttgcccaagg acaaggcgcg caccggggtc gaggccgcac
     3721 tcaccgccga gctctgttcg tgggaagccg ctgccgaaac gatcggcacc accgacacct
     3781 tcgccaaggg ctcgaccgcc agcgcgatcg ttggcggcaa aaccgtccac gtcgcgggca
     3841 tcgtcaaggg atcgggcatg atcgcgcccg acatggcgac aatgctcggc tatatcttca
     3901 ccgacgccgc ggtcgcgccg gctctgttgc aggagatgct gagcgaggcg accggcggca
     3961 gcttcaacag catcaccgtc gacagcgaca cctcgaccag cgataccgtg ctgctcttcg
     4021 cgaccgggca ggccggtaac acgccgctca cgacgcgcga cgatccgggc gccgatgcgc
     4081 tttacgcggc gatccgcacc gtcgcgctcg acctcgcgca gcaggtcgtg cgcgacggcg
     4141 agggcgcgtc gaaattcatc gaggtccaga tcaccggcgc ggtgagtgac gacagcgcca
     4201 aacgcgtcgc gctcgccatc gccaattcgc cgctcgtgaa aaccgcgatc gcgggcgagg
     4261 acgcgaactg gggccgcgtc gtgatggcgg tgggcaaggc gggcgaaccc gccgaccgcg
     4321 accgcctcgc gatccgcttc ggcgatcatt gggtcgcgaa ggacggcctg ccggtcgacg
     4381 gctatgaaga agcccccgtc gcggcgcatc tcaaagggca ggacatccgc gtcggcgccg
     4441 atctggggct cggcgaaggc cgcgcgacgg tgtggacctg cgacctcacc cacggctaca
     4501 tcagcatcaa cgccgactac cggagctgac gcccccgctc aatctgcgag cttggtccat
     4561 ttcgcgtcgc ccgacgcgtt cgcgatcacc gcttcgacga aggccatcgt ccgcaagccg
     4621 tcggcgattc cggggaacca gtcggccgcg ccgcgcggcg gcggcgtcgc ggc
//
LOCUS       DATMSU010000462        10498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_149554,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000462 DATMSU010000000
VERSION     DATMSU010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10498
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149554"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VN034_12330"
     CDS             complement(<1..504)
                     /locus_tag="VN034_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWV61440.1"
                     /translation="MTTRMLIDARHREETRVAVTKGNRIEEFDFESAEHKQLKGNIYL
                     AKVTRVEPSLQAAFVEYGGNRHGFLAFSEIHPDYYQIPKEDRDALLREEAEHAAAAAQ
                     RAEEDLDELEGDVADVEYHDDENGDAPAPETIGEDGDDEDGEAESGDAEDGEAEEGPE
                     GAGEGRGD"
     gene            1154..2107
                     /locus_tag="VN034_12335"
     CDS             1154..2107
                     /locus_tag="VN034_12335"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWV61441.1"
                     /translation="MSPLLVLIGMIGAPSAALAAPAGAIDVDPRRVATGDFEPASRAP
                     RGELQPFAGFRAKRPDKRYSVTVPIGKPKPAVALPPIEGPADARLPIVVIDAGHGGHD
                     PGAISPHDGSHEKDITLALARAIRKDLLASKRVRVALTRSDDRFLVLEERYGIARRLK
                     ADLFISVHADAAENQDANGASIYTLAEVASDREAARLAARENKANIINGVDLGAHSGD
                     VSAILLDLTQRETMNVAADFARLLQREASDEVTFRTTAHRFASFIVLKAPDTPSVLFE
                     TGFISNKDDVEFLASPAGQQKIARGVREAVQVHFARRNIAR"
     gene            complement(2144..2788)
                     /locus_tag="VN034_12340"
     CDS             complement(2144..2788)
                     /locus_tag="VN034_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWV61442.1"
                     /translation="MSKVKVAAFSISLDGYGAGREQSLQDPLGRDGERLHDWFVPTRS
                     FQKMVDKEEGTTGVDDDFGALSFENLGAWIMGRNMFGPVRGDWPDDEWKGWWGDNPPY
                     HVPVFVLTHHPRAPVTMAGGTTFHFVTDGIEAALERARDAAGDRDIRIGGGVSTIRQY
                     LKARLIDELHLAVAPVLLGGGESLFAGIDLPELGYRVTRHVAGAGATHMIVSRA"
     gene            2889..3380
                     /locus_tag="VN034_12345"
     CDS             2889..3380
                     /locus_tag="VN034_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61443.1"
                     /translation="MTNQRSGCPINLTLETLGDRWSLIVIRDIMFGNRRHFRELLTRS
                     DEGIASNILADRLKRLVEAGLLTKADDPTHKQKAVYSLTERAIDLVPLLVQMGAWGRR
                     HLPVTHAMSIRQELLEEGGPMLWNDFMDELRTLHLGAPARGDSASVLERLTTAYREAL
                     ERK"
     gene            3442..4695
                     /locus_tag="VN034_12350"
     CDS             3442..4695
                     /locus_tag="VN034_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HWV61444.1"
                     /translation="MAWSNWSGSVTASGQVARPQSEDELSMLVRSAHKLRVTGAGHSF
                     MPLCESGELIVSLDDMAGAIHIAADRRTARIPAGWSIRRLTAALWEEGLALANQGDVN
                     PQSLAGAMATGTHGTGVDLGSLSTFARGFRLVDAEGEAHWCDADTNADLYQAQRLSLG
                     LFGVATEIEVAVVPAFHLAERIEKRRWEEIYESYDELARQHRHIEYWFFPHSDDVILK
                     TLDLCDPCDPPPSTTDMEETSFRRILDVSARLPFLTPVLQRLMMKSGISGRRRGPAHS
                     IFPSDRTIHFEEMEYEMPRATGLETLAEVVGWIRKKRLPVTFPFEYRTVAADDIWMSP
                     MNAGPVAAISMHQYAKMPWAALFADAEAIFRGAGGRPHWAKRHRLSRADVAALYPMAG
                     RYTAVRRAADPRGKFLNPHLEALFA"
     gene            4692..5837
                     /locus_tag="VN034_12355"
     CDS             4692..5837
                     /locus_tag="VN034_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HWV61445.1"
                     /translation="MNDDRTLHAHLIGRQGSRADLNTPALILDIEALDRNIAAMAALT
                     AENGVALRPHAKTHKSVDIALRQLAAGAVGVCCAKIGEAEVLAGGGVTGILITSPVAA
                     PAAIERLAKLAANAEGLMAVVDHPDVAERIDAALEAAGAKLDVVIDIDPGIARTGVAS
                     AEAAVALARAIDRSPNLDYRGVQFYCGSQQHIESYADRRAAIVERTAYLQEVIAALAK
                     AGFAPGLVTGSGTGTHRIDLDLGVFTELQAGSYVFMDKQYLDCDLTGDAAPPFEVALS
                     VDARVVSANHSGLVTIDAGYKSLSTDGGVAVVQRGAPETAFFAFMGDEHAALIAPEIG
                     NTLQPGDPISLTVPHCDPTVNLYDSYHVVSGDTLVDIWPVSARGRAR"
     gene            5834..6460
                     /locus_tag="VN034_12360"
     CDS             5834..6460
                     /locus_tag="VN034_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61446.1"
                     /translation="MSVAVRRPPQQDRARQTQGRLLDVAGELLAEVGIERISTNMIAA
                     RAGLTPPALYRYFGDKYAVLEALGRRLMERQNAVLDAWLARHAPAGIAAMADHIGDLL
                     AENAAVTRAEPGAVWILRSLHASPQLVHVRLESHRHVADRLADACHVHLPRIDRAMLW
                     PRLRLAVEIGFAVDEMLYEEDRVSTEAMLAEVAAMLRRALLDLTENAS"
     gene            6520..9036
                     /locus_tag="VN034_12365"
     CDS             6520..9036
                     /locus_tag="VN034_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWV61447.1"
                     /translation="MAADSPPDFRLRLRRDGNAVITWLRDMWTRRWFRWLGYLALAGL
                     LGIALIWAIFARDLPSVDQLRDYEPPLPTMVRDGEGKPVHAYARERRVQLEYSEYPQL
                     LVRSFLAAEDKTFFSHGGIDYPGIASAIITNLSSSGRPVGASTITQQVAKNLLLTNEL
                     SYRRKVREAILAMRIEDALTKEQILELYLNEIPLGRRSFGVQAASRAYFDKDVDQLAL
                     HEMAFLAILPKAPEKYGRTRFEREALARRNFVLGAMADNGWINAAQRDAARAMPLGLT
                     DSGNRAVAQVGGYYMEEVRRQLIAEFGETAEDGPHSVYAGGLWVRTPYDGKMQEAAAM
                     ALRKGLQRYDAGKGWSGPIATIEADDQWQSRLASSFIGIDYDNWRVAAVLSKSGGEAR
                     IGFSNGDTGRLPASSAGMRYRKTGGSAFSALRPGDLIVVKSAGGDRYALRNIPEVSGG
                     MVVESPHSGRIYAMQGGFDVRLSSFNRATQAERQPGSTIKPFVYAAALDNGMTPATMI
                     VDGSFCVYQGAALGNKCFRNFGNSGGSGEHTMRWGLEQSRNLMTVRAASLVGMEPVVE
                     MISRMGIGKHEPYLSTALGAGSTTVEKITNAYAMLANHGRQLKPRVIDYAQDRRGKVI
                     FPKNWKPCEGCNMKDWDGRPMPRFAKSGKQLMDPITAYQVVHMLEGVVQRGTAQRLRD
                     LDVPLFGKTGTTSGPNDVWFVGGTPDVVAGMYIGFDQPRSMGGYAQGGSYAAPIFKDF
                     AHAALADRQPIPFSAPKGVRMVRIDRQSGRRVYGAWPGTDPKASIIWEAFKPESEPRR
                     TIREEEIKPAKPAPRQDVPVQQSPGRRSDSEFLEDRGGII"
     gene            9107..10234
                     /gene="prfB"
                     /locus_tag="VN034_12370"
     CDS             9107..10234
                     /gene="prfB"
                     /locus_tag="VN034_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWV61448.1"
                     /translation="MRAEAQDHIDKIGAALALLRRFLDWDRAVRRLDELNAKVEDPTL
                     WNDAKAAQEVMRERRRLDEAITATRAIETECADTAELIELAEMEGDEAMVDEAVASLA
                     VLAARAEEDKIKALLAGEADGNDAYIEVHAGAGGTESQDWAEMLQRMYMRWAEKRGMK
                     VELVEYQAGEQAGIKSATMLVKGENAYGYAKTESGVHRLVRISPYDSSARRHTSFSSV
                     WVYPVIDDNIEIEINEGDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGIVVASQNDR
                     SQHKNRATAMGMLKARMYEAELQKREAAASGEYQAKTEIGWGHQIRSYVLQPYQLVKD
                     LRTGVTSTAPSDVLDGALDPFMAAALSQKVTGEKVEVEDID"
     gene            10234..>10498
                     /locus_tag="VN034_12375"
     CDS             10234..>10498
                     /locus_tag="VN034_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWV61449.1"
                     /translation="MKRAAFALALLPLLGGCGAPWGDDSDRTESARDFPPADRPVAPT
                     VSTKWSTEEARDRVNEAEDVMDSADVRPGMTVADIGAGDGYYTV"
BASE COUNT         1826 a         3491 c         3454 g         1727 t
ORIGIN      
        1 gtcgccgcgg ccttccccgg ctccttctgg gccttcttcg gcttcgccgt cctcggcatc
       61 gccgctttcg gcctcgccat cctcgtcatc accatcttcg ccgatggttt cgggggcggg
      121 ggcgtcgcca ttttcgtcgt catgatattc gacgtcggcg acatcgccct cgagctcgtc
      181 gagatcctcc tcggcgcgct gcgcggcggc ggcggcatgc tcggcctctt cgcgcagcag
      241 cgcgtcgcgg tcttctttcg gaatctggta atagtcaggg tggatttcgc tgaacgcgag
      301 gaagccgtgg cgattgccgc catattcgac gaacgccgcc tgcagcgacg gttcgacgcg
      361 ggtcaccttg gccagataaa tattgccctt gagctgcttg tgctcggcgg actcgaaatc
      421 aaactcctcg attcggtttc ccttggtgac ggcgacccgg gtttcttccc ggtgccgcgc
      481 gtcgatcaac atgcgcgtgg tcattagata cactccaagc gcgcctcagc gcgccatcaa
      541 aaaatcccgc gaacctgccc gagggcgggc cgcggcggat acgggtgaaa agaaaaaaga
      601 cgttattgcc gaaaggagcc tgcgtccggt cctggacgcg catgcggtct tcaaatccgg
      661 cgacgaccgg ggcgaaagcg cccgcgtcgg gatctgaaga gggcatggca agcctcatac
      721 ggcatcaacc tgttacggga tgagcgggcg ggcagggcgg gcgcgcaagc gaacgccccg
      781 actgtccggt caaccgggtg gactggcggc aggcttgtcc ttcccgatat gtccggcgcc
      841 aaggcggcca tgatccgcat atctgcggag caggccttcc cctcaaaagg ccggacggca
      901 ccgggaacga cgccgtcagt gatggcgtgc tagcatcggc acaggccgac ggcaaccaca
      961 accaccccag aaagatagga tggcgccaag gtgttgcaag aaaaccattc ggccacgcgg
     1021 cggcccgcct tctcggccga tgtccctttg tgtcgcgatg gcgaacggcg gattcaccct
     1081 gttgtcgtgc tggaaagctg acatcttcga ataaggctgg accatcgcgc gccttggacg
     1141 gcatagacgc gccatgagcc cgctgctcgt cctgataggc atgatcggag ccccctcggc
     1201 agctttggcg gcgccagccg gtgcgattga tgtcgacccc cgccgggtcg ccacaggcga
     1261 tttcgaaccg gcatcgcggg ctccgcgcgg cgaattgcag cccttcgccg gcttccgtgc
     1321 caagcgtccg gacaaacgct acagcgtgac cgtcccgatc ggaaagccca agcccgccgt
     1381 ggcgctgccc cccatcgaag gccccgccga cgctcgcctg ccgatcgtgg tgatcgacgc
     1441 cgggcacggc ggccacgacc ccggtgcgat ctcgccgcac gacggcagcc atgaaaagga
     1501 catcacgctg gcgctcgccc gcgcgatccg caaggatctg ctcgcctcaa agcgggtgcg
     1561 cgtcgcgctc acccgctcgg acgatcgctt tctcgtgctc gaagagcgtt acggcatcgc
     1621 acggcgattg aaggccgacc tgttcatctc ggttcacgcc gacgccgccg agaatcagga
     1681 cgcaaacggc gcgtcgatct atacgctggc cgaagtcgcc tccgaccgcg aagcggcgcg
     1741 gctggcggcg cgcgagaaca aggcgaatat catcaacggc gtcgacctcg gcgcgcacag
     1801 cggcgacgta tcggcgatcc tgctcgacct cacccagcgc gagacgatga acgtcgccgc
     1861 cgactttgcg cggctgctcc agcgcgaggc gtccgacgag gttacgttcc gcaccaccgc
     1921 gcaccgtttc gcctccttca tcgtgctgaa ggcgcccgac acgccgtcgg tgctgttcga
     1981 aaccggcttc atctcgaaca aggatgacgt cgaattcctc gcctcgcccg ctggtcagca
     2041 gaagatcgcg cgcggcgttc gcgaggcggt gcaggtccac tttgcgcggc gcaacatcgc
     2101 gcgctgatat gcgcggccgg gtctgcgaaa ggcgggtagc gccctaggcc cggctgacga
     2161 tcatatgcgt cgcgcccgcg cccgcgacgt ggcgcgtcac gcgatagccg agttcgggca
     2221 ggtcgatacc cgcgaacagg ctctcgccgc cgccgagcag gaccggcgcg accgccagat
     2281 gcagctcgtc gatcaggcgc gctttcagat attggcggat cgtcgacacc ccgccgccga
     2341 tgcggatgtc gcgatcgcca gccgcatcgc gcgcgcgttc gagcgccgcc tcgatcccgt
     2401 cggtgacgaa atggaacgtc gtgccgcccg ccatcgtgac gggcgcgcgc ggatggtgcg
     2461 tcaggacgaa aaccgggaca tgatagggcg ggttgtcgcc ccaccagccc ttccattcgt
     2521 cgtcgggcca gtcgccgcgt accgggccga acatgttgcg acccatgatc cacgcgccca
     2581 gattttcaaa gctcagcgcc ccgaaatcat cgtcgacgcc ggtcgtcccc tcctccttgt
     2641 cgaccatttt ctggaacgag cgcgtgggga cgaaccagtc gtgcagcctt tcgccgtcgc
     2701 ggcccagcgg atcctgcaag ctctgttcgc gtcccgcgcc atagccgtcg agcgagatgc
     2761 tgaatgcggc aacctttacc ttggacatca tccttctcca atgtgattgt attttgcaac
     2821 cagttgtgtg ttgctagtaa atctgatttc atattgcaag cggttttatt cgggcttaga
     2881 gcggacgcgt gacgaaccaa cgctccggat gcccgatcaa cctgacgctc gagacgctcg
     2941 gcgaccgctg gagcctgatc gtcatccgcg acatcatgtt cggtaatcgc cgccatttcc
     3001 gcgaattgct gacccggtcg gacgaaggga tcgcctcgaa tatcctcgcc gatcggctga
     3061 agcggctggt cgaggccggg ctgctcacca aggccgacga cccgacccat aagcagaagg
     3121 ccgtctacag cctgaccgag cgggcgatcg atctcgtgcc gctcctcgtc cagatgggcg
     3181 cttggggccg gcggcatctc cccgtgacgc acgccatgtc gattcgtcag gaactgctcg
     3241 aggagggtgg ccccatgctc tggaacgatt tcatggacga gctgcgcacg ctccatctcg
     3301 gcgcgccggc gcggggcgat tcggcgagcg tgctcgagag attgaccaca gcctatcgcg
     3361 aggctctgga acggaaataa tatagtcttg actaactttt ctgccggagc cagctttgcg
     3421 ggcgccggtc gggagagaaa tatggcatgg agcaactggt cgggaagcgt cacagccagc
     3481 gggcaagtcg cccgtccgca aagcgaggac gagctttcga tgctcgtccg aagcgcgcac
     3541 aagctgcgcg tcaccggagc cgggcacagt ttcatgccgc tctgcgaatc gggcgagctg
     3601 atcgtcagtc tcgacgacat ggcgggcgcg atccacatcg ccgccgatcg ccggaccgcg
     3661 cgcattcccg cgggctggag catccgccgg ctcaccgcgg ctttgtggga ggaggggctg
     3721 gcgctcgcca atcagggcga cgtcaatccg cagtcgctcg ccggcgcgat ggcaacgggc
     3781 acgcacggca ccggcgtcga cctcggttcc ttatcgacct tcgcgcgcgg tttccggctc
     3841 gtcgatgccg aaggcgaggc gcattggtgc gacgccgaca ccaacgcgga cctctatcag
     3901 gcgcagcgcc tgtcgctcgg actgttcggc gttgcgaccg aaatcgaggt cgcggtcgtg
     3961 ccggcctttc acctagccga gcggatcgag aagcgccgct gggaagagat atacgaaagc
     4021 tatgacgagt tggcgcggca gcaccgccat atcgaatatt ggttcttccc gcacagcgac
     4081 gatgtgatcc tcaagacgct cgacctctgc gatccgtgcg atccgccgcc gagcaccacc
     4141 gacatggagg agacgagctt tcggcgtatc ctcgacgttt cggcgcggct gccgttcctg
     4201 acgcccgtgc tccagcggtt gatgatgaaa agcggcatct cgggccgccg ccgcgggccc
     4261 gcgcacagca tcttcccgtc ggaccgcacg atccacttcg aggagatgga atatgagatg
     4321 ccgcgcgcga ccgggctcga gacgctggct gaagtcgtcg gctggattcg caagaaacgg
     4381 ctgcccgtca ctttcccatt cgaatatcgc accgtcgccg ccgacgatat ctggatgagc
     4441 ccgatgaacg ccgggcccgt cgccgcgatc tcgatgcacc aatatgcgaa aatgccttgg
     4501 gcggcgctgt tcgccgacgc cgaggcgatc tttcgcggcg cgggcgggcg gccgcattgg
     4561 gcgaagcgcc ataggctgag ccgcgccgac gtcgccgcgc tttatccgat ggcggggcgc
     4621 tatactgcgg tgcgccgcgc cgccgacccg cgcggcaaat tcctgaaccc tcatctggag
     4681 gccctctttg catgaatgac gaccggacgc tgcacgccca tctgatcggc cgccaaggct
     4741 cgcgcgccga cctcaacacg cccgcgctca tcctcgatat cgaggcgctc gaccgcaata
     4801 ttgctgcgat ggccgccctc accgcggaga acggcgtcgc gctgcgcccg catgccaaga
     4861 cgcacaagag tgtcgatatc gcgctgcgtc agctcgcggc gggggcggtc ggggtctgct
     4921 gcgccaagat cggcgaggcc gaggtgctgg ccgggggcgg cgtgactggt atccttatca
     4981 cctcgcccgt tgccgcgccg gcggcgatcg aaaggctcgc gaagcttgcg gcgaacgctg
     5041 agggtctgat ggcggtggtc gatcatccgg atgtcgccga gcgcatcgac gcggcgctgg
     5101 aggcggcggg cgcaaagctc gacgtcgtca tcgacatcga ccccggcatc gcgcgcaccg
     5161 gcgtcgcctc ggccgaggcc gcggtcgcgc tggcccgtgc gatcgaccgg tcgcccaacc
     5221 tcgattatcg cggcgtgcaa ttttactgcg ggtcgcagca gcatatcgaa agctacgccg
     5281 accgccgcgc cgcgatcgtc gagcgcaccg cctatttgca ggaggtgatc gcggcgctgg
     5341 ccaaagcggg cttcgcgccc ggcctcgtca ccgggtcggg taccggcacg caccggatcg
     5401 atctcgatct cggcgtcttc accgaactgc aggcgggatc ctacgtcttc atggacaagc
     5461 aatatctcga ttgcgacctg accggcgacg ccgcgccgcc gttcgaggtt gcgctgtcgg
     5521 tcgacgcgcg cgtcgtcagc gcgaaccaca gcggtctcgt gacgatcgac gccgggtaca
     5581 agtcgctctc gaccgacggc ggcgtggcgg tggtccagcg cggtgcgccc gaaaccgcct
     5641 tcttcgcctt catgggggac gaacatgccg cgctgatcgc gcccgaaatc ggaaacaccc
     5701 tccagccggg cgatcccatc agcctgaccg tgccgcactg cgacccgacg gtgaatctct
     5761 atgatagcta ccatgtcgtt tcgggcgata cgctggtcga tatttggccg gtcagcgcgc
     5821 gcggccgcgc gcgatgagcg tcgccgttcg ccgcccgccg cagcaggacc gcgcgcgcca
     5881 gacgcagggg cgcttgctcg acgtcgcggg cgaattgctc gccgaggtcg ggatcgagcg
     5941 gatttcgacc aatatgattg cggcgcgcgc cgggctgacg ccgcccgcgc tctaccgcta
     6001 tttcggcgac aaatatgcgg tactcgaagc gctcgggcgg cggctgatgg agcgccagaa
     6061 cgccgtgctc gatgcctggc tcgcgcgcca tgcgccggcc ggaatcgccg ccatggccga
     6121 ccatatcggc gatctacttg ccgaaaacgc cgcggtgacg cgcgccgagc cgggggcggt
     6181 gtggatattg cggtcgctcc acgcgtcgcc gcaactcgtc catgtccggc tcgaatcgca
     6241 ccggcatgtc gccgaccggc tagcggacgc ttgccacgtg caccttcccc gcatcgaccg
     6301 cgctatgctg tggccgcgcc tgcggctcgc ggtcgagatc ggcttcgcgg tcgatgaaat
     6361 gctttacgag gaggatcgcg tttccaccga ggcgatgtta gcagaagtcg ccgcgatgct
     6421 gcggcgggcg ctgctcgacc tgaccgaaaa cgcctcatag cccgcctcgc cgcaaccatg
     6481 cttccctttc ggcgacacag gctttagagg cgggggcata tggccgccga cagtccgccc
     6541 gatttccgcc ttcgcctccg ccgcgacggc aatgccgtca tcacctggct gcgcgacatg
     6601 tggacgcggc gctggtttcg ctggctcggc tatctcgcgc tcgcgggcct gctgggtatc
     6661 gcgctgatct gggcgatttt cgcgcgcgat ctgccttcgg tcgaccagct tcgcgactat
     6721 gagccgccgc tgccgacgat ggtccgcgac ggcgagggca agccggtgca cgcctatgcc
     6781 cgcgaacgcc gcgtccagct tgaatatagc gaatatccgc aactcctggt gcgctccttc
     6841 ctcgccgccg aggacaagac cttcttcagt cacggcggta tcgactatcc ggggatcgct
     6901 tcggcgatca tcaccaatct gtcgagcagc ggccggcccg tcggcgcctc gacgatcacg
     6961 cagcaggtcg cgaaaaatct gctgctcacc aacgagctca gctatcgccg caaggtgcgc
     7021 gaggcgatcc ttgcgatgcg catcgaggat gcgctgacca aggagcagat tctcgaatta
     7081 tatctcaacg aaatcccgct cggccgtcgc agcttcggcg tgcaggcggc gagccgcgcc
     7141 tatttcgaca aggatgtcga ccagctcgcg ctgcacgaaa tggccttcct cgcgatcctg
     7201 cccaaggcgc ccgagaaata tggccgcacg cgtttcgagc gcgaggcgct ggcgcggcgc
     7261 aacttcgtgc tcggcgcgat ggcggacaat ggctggatca acgcggcgca gcgcgacgcg
     7321 gcgcgcgcga tgccgcttgg cctgaccgac agcggcaacc gcgcggtcgc acaggtcggc
     7381 ggctattata tggaagaggt ccggcgccag ctgatcgccg aattcggcga aacggcggag
     7441 gatggcccac acagcgtcta tgccggcggc ctctgggtgc gcacgccgta tgacggaaag
     7501 atgcaagagg ccgcggcgat ggcgctgcgc aagggactcc agcgctatga tgcgggcaag
     7561 ggctggtcgg ggccgatcgc gacgatcgag gccgacgacc agtggcagag ccgcctcgca
     7621 tcgagcttca tcggcatcga ttacgacaat tggcgcgtcg ccgctgtgct ctcgaaatcg
     7681 ggcggagagg cgcggatcgg cttttcgaac ggcgacaccg gacgcctgcc cgcgagttcg
     7741 gcggggatgc gctatcgcaa gaccggcggc agcgcctttt cggcgctgcg cccgggcgat
     7801 ctgatcgtcg tcaaatcggc gggcggcgat cgctatgcgc ttcgcaatat ccccgaggtg
     7861 tcgggcggca tggtcgtcga aagcccgcat tcgggccgca tctatgcgat gcagggcggc
     7921 ttcgacgtcc gcctgtcgtc gttcaatcgc gcgacgcagg ccgaacgcca gccgggctcg
     7981 acgatcaagc ccttcgttta tgccgccgcg ctcgacaacg gcatgactcc cgcgacgatg
     8041 atcgtcgacg ggtccttctg cgtctatcag ggcgcggcgc tcggcaacaa atgcttccgc
     8101 aacttcggca attcgggcgg cagcggcgag cacacgatgc gctggggcct cgaacagtcg
     8161 cgcaacctga tgacggtgcg cgcggcgagc ctcgtcggca tggaaccggt cgtcgagatg
     8221 atcagccgca tgggcatcgg caagcacgaa ccctatcttt cgaccgcgct cggcgccgga
     8281 tcgacgacgg tcgaaaagat caccaacgcc tatgcgatgc tcgccaatca cggccgccaa
     8341 ctgaagccgc gggtgatcga ttatgcgcag gaccgccgcg gcaaggtgat ctttccgaag
     8401 aactggaagc cctgcgaggg ctgcaacatg aaggactggg acggccggcc gatgccgcgc
     8461 ttcgccaagt cgggcaagca gctgatggac ccgatcaccg cctatcaggt cgtgcatatg
     8521 ctcgaaggcg tcgtccagcg cggcaccgcg cagcggctgc gcgacctcga tgtcccgctg
     8581 ttcggcaaga cggggacgac ctcggggccg aacgacgtct ggttcgtcgg cggcacgccc
     8641 gacgtggtcg cgggcatgta tatcggtttc gaccagccgc gcagcatggg cggctatgcc
     8701 cagggcggca gctatgcggc gccgatcttc aaggatttcg cacatgcggc gctcgccgac
     8761 cgccagccga ttcccttttc ggcgccgaag ggcgtgcgca tggtgcggat cgatcgccag
     8821 tcggggcgcc gcgtctatgg cgcctggccg ggcaccgatc ccaaggcatc gatcatctgg
     8881 gaagccttca agcccgaaag cgagccgcgg cggacgattc gcgaagaaga gatcaaaccg
     8941 gccaagccgg cgccccggca ggacgtgccc gtccagcaaa gcccgggccg gcgcagcgac
     9001 agcgaattcc tggaggatcg cggcggcatc atctgacgcg gcgcacctcc ttcccctgcg
     9061 gccttcgagg ccgtaagggc ggaccaaatt attcaggagc aaggacatgc gcgccgaagc
     9121 gcaggatcat atcgacaaga ttggcgccgc gctggcgctg ttgcgccgct ttctcgactg
     9181 ggaccgcgcg gtgcggcggc tcgacgaact caatgcgaag gtcgaggacc cgacgctgtg
     9241 gaacgacgcc aaggccgcgc aagaggtcat gcgcgaacgc cgccggctcg acgaggcgat
     9301 caccgcgacg cgtgcgatcg agaccgagtg cgccgatacc gccgagctga tcgaactcgc
     9361 cgagatggaa ggcgacgagg cgatggtcga cgaggcggtg gcctcgctcg ccgtgctcgc
     9421 cgcgcgcgcc gaggaagaca agatcaaggc gctgctcgcg ggcgaggccg acggcaacga
     9481 cgcctatatc gaagtccacg ccggtgccgg cggcaccgag agccaggact gggccgagat
     9541 gcttcagcgc atgtatatgc gctgggcgga aaagcgcggg atgaaggtcg agctcgtcga
     9601 atatcaggcg ggcgaacagg cgggcatcaa gtcggcgacg atgctggtga agggcgagaa
     9661 tgcctatggc tatgccaaga ccgaaagcgg cgtccaccgc ctcgtccgca tctcgcccta
     9721 cgatagctcg gcgcggcgcc acaccagctt ttcgagcgtc tgggtctatc cggtgatcga
     9781 cgacaatatc gagatcgaga tcaacgaggg cgacctcaag atcgacacct atcgcgcatc
     9841 gggcgcgggc gggcagcacg tcaacacgac cgattcggcg gtgcgtatca cccacatccc
     9901 gaccgggatc gtcgtcgcat cgcagaacga ccgttcgcag cacaagaacc gcgcgaccgc
     9961 gatggggatg ctgaaggcgc ggatgtacga ggccgagctc cagaagcgcg aggccgcggc
    10021 gagcggagaa tatcaggcga agaccgagat cggctggggc caccagatcc gctcctatgt
    10081 cctccagccc tatcagctgg tgaaggacct caggaccggc gtgacctcga cggcgccgtc
    10141 cgacgtgctc gacggcgcgc tcgatccctt catggcggcg gcgctgtcgc agaaggtgac
    10201 gggcgaaaag gtcgaggtgg aggacatcga ttgatgaagc gcgctgcctt cgccctcgcg
    10261 ctcctgccgc tgctcggcgg ctgcggggcg ccatggggcg acgacagcga ccgcaccgag
    10321 agcgcgcgcg attttccacc cgccgaccgg ccggtggcgc cgaccgtatc gaccaaatgg
    10381 tcgaccgagg aggcgcgcga ccgcgttaac gaggccgagg atgtcatgga ctcggccgac
    10441 gtccgccccg gaatgaccgt cgccgatatc ggcgcgggcg atggctatta tacggtgc
//
LOCUS       DATMSU010000463         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_149593,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000463 DATMSU010000000
VERSION     DATMSU010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149593"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..707)
                     /locus_tag="VN034_12380"
     CDS             complement(273..707)
                     /locus_tag="VN034_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61450.1"
                     /translation="MKQGLALPLAALLTLSLAACSQPPPPTDNAPVVAGEPIATRAVM
                     IGTEGPSLPACSSVSRVKAGGTDVYWAPNETRAVKAKLAGGANVSLCEAAENDAWFGV
                     VFAAPGTDPDMCGVGKSVPNAREYQGPCRWGWIKGGTVQLGG"
     gene            815..>2710
                     /locus_tag="VN034_12385"
     CDS             815..>2710
                     /locus_tag="VN034_12385"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HWV61451.1"
                     /translation="MTDTPENTAPVAPANQPNANAYGADSIKVLKGLDAVRKRPGMYI
                     GDTDDGSGLHHMVFEVSDNAIDEALAGHCDLVLITLNSDGSVSVEDNGRGIPTGIHAE
                     EGISAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGE
                     EHWMRFEHGDSVAPLKVNGPAPAGKKGTRVTFCASTDTFKNVTEFDFEKLEHRYRELA
                     FLNSGVRIKLVDARHAEHETHDLFYEGGIGAFVKYLDRNKNALLPDPIAISSERDGIG
                     IDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNGYGDKSGILKKEKVS
                     LTGEDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADRMTEWLEENPAH
                     AKAVIQKVIDAAAAREAAKKARELTRRKGAMDIASLPGKLADCQERDPSKCELFLVEG
                     DSAGGSAKQGRDRHVQAILPLKGKILNVERARFDRIISSKEVGTLIQALGTGIRDEFN
                     LEKLRYHKIVIMTDADVDGAHIRTLLLTFFYRQMPEIIENGHLYIAQPPLYKVAKGRS
                     EVYLKDDTALENYLVDAGIDALLLETAGGARSGADLRSLIDHGRRLRALMRYVPRTLN
                     HGLVEALA"
BASE COUNT          500 a          877 c          888 g          445 t
ORIGIN      
        1 gctcgaaacc ccgggcggcg gcgcgatgac gctgccggtg cagtcggaga tcgggcgggt
       61 gatcgaggat tgccgggggt aggggcgtcg gcttcggggt ggggagttgc cgttcatgca
      121 ccctctggtg caccccggcg aaagccgggg cccagtgcgg aaaagcgcca ggtcagcgtc
      181 agcattctgg gccccggctt tcgccggggt tcacatctct atcggcgcct tccggccgaa
      241 accggtcata tcggtggcgc gcgcggccgg gcctatccgc ccagctggac cgtcccgccc
      301 ttgatccagc cccagcggca cgggccctga tattcgcgcg cgttggggac cgacttgccg
      361 acgccgcaca tgtcgggatc ggtgccgggc gcggcgaaga cgacgccgaa ccaggcgtcg
      421 ttctcggccg cttcgcacag cgacacattc gcgccgccgg cgagcttcgc cttgaccgcg
      481 cgcgtctcgt tcggcgccca atagacgtcg gtgccgcccg ccttgacgcg gctgacgctc
      541 gaacaggcgg gcaggctcgg cccttcggtg ccgatcatca ccgcgcgcgt cgcgatgggt
      601 tcgcccgcaa cgacgggggc gttgtcggtc ggtgggggcg gctggctgca cgcggcgagc
      661 gagagggtga ggagcgcggc gagggggagg gcgagacctt gtttcatgcc tgcgaaacta
      721 gccgccgcgg cgtcgccgcg caacgctcgt cgcccaagcc ccaagagccg cattttcgct
      781 tgggtttttg cgccttcgac cctatataat cgatatgacg gacaccccag aaaatacggc
      841 gccggtcgcg cccgccaacc agcccaacgc caacgcctat ggcgccgatt cgatcaaggt
      901 tctgaagggc ctcgacgcgg tccgcaaacg cccgggcatg tatatcggcg acaccgacga
      961 cggctcgggg ctgcatcaca tggtgtttga ggtttcggac aatgcgatcg acgaagcgct
     1021 cgcggggcat tgcgatctcg tcctgatcac attgaacagc gacggttcgg tcagcgtcga
     1081 ggataatggc cgcggcatcc cgacgggcat ccatgccgag gaaggcattt cggcggccga
     1141 ggtcatcatg acccagcttc acgcgggcgg gaagttcgag aatacgagcg acgacaatgc
     1201 gtacaaggtg tcgggcggcc tgcacggcgt cggcgtctcg gtggtcaacg cgctgtcgga
     1261 atggctcgag ctcacgatct ggcgcgacgg cgaagagcat tggatgcgct tcgagcatgg
     1321 cgattcggtc gcgccgctca aggtcaatgg ccccgcgccg gcgggcaaga agggcacgcg
     1381 cgttactttc tgcgcctcga ccgacacgtt caagaacgtc accgaattcg atttcgaaaa
     1441 gctcgagcat cgctatcgcg agctcgcctt cctgaactcg ggcgtgcgca tcaagctcgt
     1501 cgacgcgcgc catgccgagc atgaaacgca cgaccttttc tacgagggcg ggatcggagc
     1561 gttcgtcaaa tatctcgacc gcaacaagaa tgcgctgctc cccgatccga tcgcgatcag
     1621 cagcgagcgc gacggcatcg gcatcgacgt cgcgctcgaa tggaacgaca gctattatga
     1681 aaatgtcctc tgcttcacga acaacatccc gcagcgcgac ggcggcacgc acctcgcagc
     1741 gttccgcgcc gcgctgacgc gcacattgaa cggctatggc gacaaatcgg gcatcctcaa
     1801 gaaagagaag gtctcgctga ccggcgagga tatgcgcgaa ggtctgaccg cgatcgtttc
     1861 ggtcaagctg cccgatccca aattctcctc gcagaccaag gacaagctgg tttcctccga
     1921 agtccgccag ccgctcgaaa gcctgatggc cgaccggatg accgaatggc tcgaggaaaa
     1981 tcccgcccac gccaaggcgg tgattcagaa ggtgatcgac gccgccgcgg cgcgcgaagc
     2041 cgcgaagaag gcgcgcgagc tgacgcggcg caagggcgcg atggacatcg cctcgctccc
     2101 cggcaagctc gccgactgcc aggagcgcga cccctcgaaa tgcgaactct tcctcgtcga
     2161 gggtgactcg gcgggcggct cggcgaagca gggccgcgac cggcatgtgc aggcgatcct
     2221 gccgctcaag ggcaagattt tgaacgtcga gcgcgcgcgt ttcgaccgca tcatctcgtc
     2281 gaaggaagtc gggacgttga tccaggcgct cggcaccggg atccgcgatg aattcaacct
     2341 cgaaaaactg cgctatcaca agatcgtgat catgaccgac gccgacgtcg acggcgcgca
     2401 tatccgcacg cttctgctca cctttttcta tcgccagatg cccgagatca tcgagaacgg
     2461 ccatctctac atcgcccagc cgccgctcta caaggtcgcg aaggggcgga gcgaagtcta
     2521 tctcaaggac gataccgcgc tcgaaaatta tctcgtcgat gcggggatcg atgcgttgct
     2581 gctcgaaacc gcgggcggcg cgcgctcggg cgccgacctg cgctcgttga tcgatcacgg
     2641 ccggcggctg cgcgcgctga tgcgttatgt gccgcgcacc ctcaatcacg ggctggtcga
     2701 ggcgctcgcg
//
LOCUS       DATMSU010000464         5858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_8381, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000464 DATMSU010000000
VERSION     DATMSU010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8381"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2031)
                     /locus_tag="VN034_12390"
     CDS             complement(61..2031)
                     /locus_tag="VN034_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWV61452.1"
                     /translation="MRSKTHLFRSAAFAAMGALLLTSCGGGGGGGSTPTPTPTPTPAP
                     TPTTVYTPPAAEALTTADVERVLAQAISEAQARSLPSVIAVTDRVGNVLGVFRMTGAR
                     ATATTASAPNGVNIDAQNVTFPAEGGAIAKAVTGAYLSSGGNAFSTRTASQIVQQHFP
                     PAPTTVGLESGPLFGVQFSQLPCSDLSARFGAAGNAALIGPKRSPLGLAADPGGLPLY
                     KNGVLVGGIGVMGDGVYGSDANILDIDNDPEEFIALAGTRGFEAPSTITADKITVDGT
                     SLRFSDATFAGVMSSGGASFASLNGTAGNLVAVIGYANAAVTAGVAYGSEASGVRAST
                     PAEFSNRDAFVLTDGSGINRYPLRAGTDGASNSAPLTQAEARAVLEEAFTVMSRARAQ
                     IRRPLDSRAQVTISIVDTHGSVLGIVRSPDAPIFGTDVSLQKARTAAFFSGAQAGAEL
                     GANASADVRQFVTATRTFLNNPAALTGTIAFSDRAGGNLSRPYFPDGEVGRPQGPLSR
                     PIAQFNPFSTGLQSALIIGNLGAHLGFVSGASPTDTPQRCTTLPDVAAGQNRLQNGIQ
                     IFPGSVPIYRGNQLVGAIGVSGDGIDQDDMISFLGAHNGGARVGGIGNAPAAIRADTV
                     VVNVGAGVRLRYISCPFAPFLDTANQNVCNGL"
     gene            complement(2036..3463)
                     /locus_tag="VN034_12395"
     CDS             complement(2036..3463)
                     /locus_tag="VN034_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HWV61453.1"
                     /translation="MTTHAGKERADRPWAVFAALTLLALAIAAFVFAAPARSQGESGA
                     RYTGVASCAGSTCHGRMEGDGTVVRQDELMKWQEPSTPGGAHSRAWAVLSNNRSQFIA
                     RNLGIGDPSKAQMCLGCHSTAGTARAVPAEDGVGCESCHGPAGGWLASHYAGVGTNAD
                     PDREMRQKHLANLSAGLKKLEDPVVRAGVCVDCHFGSAADGQFVTHRIMAAGHPRIAF
                     ELDLFSSLQAHHQEDADYGWRKFGAANRRTDHVQMWAVGQATAIERSLALFQTRRGAE
                     GMFPEFYFLDCHSCHRRIFDQAKPVRTGLDNPGRSIPEGMPPYNDENLIMLAAAARMA
                     SPSLADQLAVRTAAFHKAMATDRPSAVAAAAQLAQTVAALKSAFASRNFSGADAFAMV
                     DAISAKAIGDRYTDYSGSQQAVMGVDTLLNAMVSSGRVTVGAAAGIRGDIDRAYAAVK
                     DPNAYKPSEFQASFGSAVRAIGALR"
     gene            complement(3513..4868)
                     /gene="gdhA"
                     /locus_tag="VN034_12400"
     CDS             complement(3513..4868)
                     /gene="gdhA"
                     /locus_tag="VN034_12400"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HWV61454.1"
                     /translation="MAVSDHVDFLTFMEGVKKRNPGQPEFVQAVQEVSEDIFDFIADK
                     EEYHAQQILRRIAEPDRVVSFRVCWEDDNGNIRVQRGWRVQNNNAIGPYKGGIRFHPS
                     VTESVLKFLAFEQTFKNALTGLPMGGGKGGSNFNPKGKSVREIMRFCQSFMTELYRHI
                     GADIDVPAGDIGVGGREIGFMFGQYKRITNEFTGVLTGKGLEWGGSLIRTEATGYGAV
                     YFLANMLAAKGQDLVGKTAVISGSGNVATHAAEKIVQLGGKVLTLSDSGGFIHDPDGI
                     TQEKIDWVKAHKTHRRGRIEEYCDEYKSASFTAGKTPWGVKCDVALPCATQNELLGDD
                     AKALVANGCIAVSEGANMPTNLEGVHVFKDAKIMFAPGKAANAGGVAVSGLEMSQNSG
                     RRAWSEGELQQMLKDIMDGIHKSCITYGDQGDGYIDYVKGANIAGFKKVADAMLAFGV
                     V"
     gene            5028..>5858
                     /locus_tag="VN034_12405"
     CDS             5028..>5858
                     /locus_tag="VN034_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWV61455.1"
                     /translation="MSETNGMKSRMTGTNRAILIAAFVLLAAAIGYAIWRDSAASPAN
                     PIAENAAMPSDQLAALEARTKSEPDSADAWTALGAARFDMSDFAGAVAAYQKAVTLAP
                     ESAGLWSALGEARVMGSDGEPMPAAASQAFDKAIALDPKDPRARYFLAVRKDIGGDHK
                     GAIEDWFALLADTPQGAPWEADLRRTIEQVGQIHKIDVTARLAATQARPLTPNEMPVA
                     ARAIPGPSRADMEAASQLPQGQQDQMIEGMVSGLEAKLKANPADVDRWIMLMRSRMTL
                     G"
BASE COUNT         1001 a         1931 c         1927 g          999 t
ORIGIN      
        1 cgcgccctgc gccgcgagtg tcgcgatgat cggccaaagg ctgccccccg tcatcatcgc
       61 ctatagcccg ttgcagacat tctggttcgc cgtatcaagg aaaggggcga acgggcagct
      121 gatgtaacgc aggcgaaccc cggcgccgac atttacgacg acggtgtccg cacggatcgc
      181 ggccggcgca ttgccgatgc cgccgacgcg cgcgccgcca ttatgcgcac cgaggaagct
      241 gatcatgtcg tcttgatcga tgccgtcgcc cgacacaccg atcgccccga ccagttggtt
      301 gccgcgatag atggggaccg atccggggaa gatctgaatg ccgttctgca gccggttctg
      361 ccccgcggca acgtcgggca aggtcgtgca gcgctgcgga gtatcggtcg gactggcgcc
      421 cgaaacgaag ccgagatgtg cgccgagatt gccgatgatc agcgccgatt gcagcccggt
      481 cgagaagggg ttgaactgtg cgatcgggcg cgacagcggc ccttgcggcc ggccgacctc
      541 gccatcgggg aaatagggcc gcgacagatt gccacccgca cggtcggaaa aggcgatcgt
      601 gccggtgagc gcggccggat tgttcagaaa ggtgcgtgtc gcggtgacga actggcgcac
      661 gtcggcgctg gcattggcgc caagttcggc tcccgcctgc gcgcccgaga agaaggcggc
      721 ggtccgcgct ttctgcaagg acacgtcggt gccgaagatc ggggcatcgg gcgaacgtac
      781 gataccgagc accgacccgt gcgtgtcgac gatgctgatc gtcacctgcg cccggctgtc
      841 gagcgggcgg cggatttgcg cgcgcgcacg gctcatcacc gtaaaggctt cctcgagtac
      901 cgcgcgcgcc tcggcctgcg tcagcggcgc gctgttgctc gcgccatcgg tgccggcgcg
      961 gagcggatag cggttgatgc cgctgccgtc ggtgagcacg aaggcatcgc gattcgagaa
     1021 ttcggccggc gtcgaggcgc ggacgcccga cgcttcgctg ccataggcca ccccggcggt
     1081 caccgcggcg ttggcatagc cgatcaccgc gacaagattg cccgcggtgc cgttgagact
     1141 ggcgaagcta gcgccgccgc tcgacatcac tccggcgaag gtcgcgtcgg agaaacgcag
     1201 gctcgtcccg tcgacggtga tcttgtcggc ggtgatcgtc gaaggcgcct cgaaaccgcg
     1261 tgtaccggcg agcgcgatga actcctcggg gtcattgtcg atgtcgagga tgttggcatc
     1321 gctgccgtag acgccatcgc ccatcacccc gatgccgccg acgagcacgc cattcttgta
     1381 gagcggcagt ccgccggggt cggcggcgag gcccagcggc gagcgtttgg gtccgatcag
     1441 ggcggcgtta cctgcggcac cgaaacgcgc cgacaggtcg ctgcacggaa gctggctgaa
     1501 ctgcacaccg aagagcggtc cgctttcgag gcccacggtg gtgggcgcag gcgggaaatg
     1561 ttgctgcacg atctggctcg cggtgcgcgt cgagaaagcg ttgccgccgc tcgagaggta
     1621 cgcgccggtg accgccttgg cgatcgcgcc gccctcggcc gggaaagtga cgttctgcgc
     1681 gtcgatattc acgccattgg gtgcggacgc ggtcgttgcg gtcgcgcgtg cgccggtcat
     1741 ccggaagacg ccgagcacat tgccgacgcg atcggtcacc gcgatcaccg acggcaggct
     1801 gcgcgcctgc gcttccgata tggcctgcgc gagcacgcgc tcgacgtccg ccgtggtcag
     1861 cgcctcggcg gcgggcggcg tatacaccgt agtcggcgtt ggagcggggg tcggcgtggg
     1921 tgtcggggtc ggcgtactgc caccgcctcc accaccgccg cacgaggtga gcagcaacgc
     1981 gcccatcgca gcgaaggcgg ccgaacggaa aagatgggtc ttgcttcgca tgatcttacc
     2041 gcagcgctcc gatcgcgcgc acagcgctgc cgaacgaagc ctggaattcg gagggtttgt
     2101 acgcgttagg gtccttcacc gcggcgtaag cacggtcgat gtcgccccgg atgcccgccg
     2161 ccgcgccgac ggtgacgcgg cccgacgaca ccatcgcatt gagcaaggtg tcgaccccca
     2221 tcaccgcctg ctgcgacccc gaatagtcgg tgtagcgatc gccgatcgcc ttcgccgaaa
     2281 tcgcatcgac catcgcaaag gcatcggcgc ccgaaaaatt gcgcgacgcg aatgcacttt
     2341 tgagcgccgc gaccgtctgc gccagctgcg ccgcggcggc gaccgcactc ggccggtcgg
     2401 tcgccatcgc cttgtggaac gccgcggtgc gtaccgcgag ctggtcggcg agcgaaggcg
     2461 acgccatacg tgccgccgcc gccagcatga tcagattctc gtcattatag ggcggcatgc
     2521 cttcggggat cgagcggccg ggattgtcga gccccgtgcg caccggcttc gcctggtcga
     2581 agatgcggcg gtgacagctg tggcaatcga ggaagtaaaa ttcggggaac atgccctcgg
     2641 ccccgcgccg cgtctgaaac agcgcgaggc tgcgctcgat cgcggtcgcc tgccctaccg
     2701 cccacatctg gacatgatcg gtgcgccggt tcgcggcacc gaacttgcgc cagccatagt
     2761 cggcgtcttc ctgatggtgc gcctggagcg aagagaagag gtcgagttcg aaggcgatgc
     2821 gcgggtggcc ggcggccatg atgcggtggg tgacgaactg accgtcggcg gccgagccaa
     2881 agtggcagtc gacgcacacg cccgcgcgca cgaccggatc ttcgagcttc ttgagccccg
     2941 ccgacaggtt cgcgagatgt ttctggcgca tttcgcggtc gggatcggcg ttcgtcccga
     3001 ccccggcata gtggcttgcg agccagccgc ccgccggacc atggcacgat tcgcagccga
     3061 cgccatcttc ggcgggaacc gcgcgcgcgg tgcccgcggt gctgtggcag ccgaggcaca
     3121 tctgcgcctt ggatgggtca ccgatgccga gattgcgcgc gatgaactgg ctgcggttat
     3181 tgctgagcac cgcccacgcg cggctatgcg cgccgccggg ggttgacggt tcctgccatt
     3241 tcatcagctc gtcctggcgg acgacggtgc cgtcaccctc catccggccg tggcaggtcg
     3301 accccgcgca cgaggcgacg ccggtatagc gcgcgccgct ttcgccctgc gaccgggcgg
     3361 gggctgcgaa gacgaacgcc gcaatggcaa gcgcgagcag cgtcagggcg gcaaataccg
     3421 cccaaggccg atcggcgcgc tcctttccag cgtgcgtcgt catccgcaga accccttttc
     3481 cccaacccgg cgcttttacg ccttgagccg aattacacca ccccgaaagc cagcatcgcg
     3541 tcggcaacct tcttgaatcc cgcgatattg gcgcccttta cataatcgat atagccgtcg
     3601 ccctgatcgc cataagtaat gcagctcttg tggatgccgt ccatgatgtc cttcagcatc
     3661 tgctgcaact cgccttcgct ccaggcgcgg cgcccgctgt tctggctcat ttcgagcccc
     3721 gacacagcaa cgccgccggc attcgccgcc ttgccgggtg cgaacatgat cttcgcgtcc
     3781 ttgaagacat gcacgccttc gaggttcgtc ggcatgttcg cgccctcgct gaccgcgatg
     3841 catccgtttg cgaccagcgc cttggcatcg tcgccgagca attcgttctg cgtcgcgcac
     3901 ggcagcgcaa cgtcgcactt cacgccccac ggcgtcttgc ccgcagtgaa gctcgccgat
     3961 ttatactcgt cgcaatattc ttcgatccgg ccgcggcgat gcgtcttgtg cgccttcacc
     4021 cagtcgatct tttcctgcgt gatgccgtcg ggatcgtgga tgaagccgcc cgaatcggag
     4081 agcgtcagta ccttgccacc gagctggacg atcttttcgg cggcatgcgt cgcgacattg
     4141 cccgatcccg aaatcaccgc cgtcttgccg acgagatcct gccccttcgc cgcgagcatg
     4201 ttggcgagga agtaaaccgc gccatagccc gtcgcctcgg tgcggatcag cgagccgccc
     4261 cattcgaggc ccttgccggt gagcacgccg gtaaattcgt tggtgatgcg cttgtactga
     4321 ccgaacatga aaccgatctc gcgcccgccg acgccgatgt cgcctgcggg cacgtcgatg
     4381 tcggcgccga tgtgacgata aagttcggtc atgaagctct ggcaaaagcg catgatctcg
     4441 cgcacgctct tgcccttggg attgaagttc gatccgccct tgccgccgcc catgggtagc
     4501 ccggtcagcg cgttcttgaa cgtctgttcg aaggcgagga atttcagcac gctctcggtg
     4561 acgctgggat gaaagcggat cccgcccttg taggggccga tggcattgtt gttctggacg
     4621 cgccagccgc gctggacgcg gatattgccg ttgtcgtctt cccagcacac gcgaaaggac
     4681 acgacccggt cgggttcggc gatccgccgc aagatttgct gcgcgtgata ttcttccttg
     4741 tcggcgatga aatcgaagat gtcttcggac acctcctgca ccgcctgcac gaactcgggc
     4801 tgacccgggt tccgcttttt cacgccttcc atgaacgtca aaaaatcgac gtgatccgaa
     4861 actgccatgc acccctcccc tgatgaccgg gaaagccggt gcgaccccgt tgacgatttt
     4921 atcgttgacc gaccttgtat tccaaaggct acaccatcgc caagcaaagg caatatgaca
     4981 gcgggcacag gcggcatttt ttcgcggcgc gcacaagagg ggcaaaaatg agcgagacca
     5041 atggcatgaa gagccgcatg acgggcacca accgggcgat attgatcgcc gcattcgtgc
     5101 tgttggctgc cgcgatcggc tatgcgatct ggcgcgattc cgctgcctcg ccggccaacc
     5161 cgattgccga aaacgccgcg atgccgtcgg accagctcgc ggcactggaa gcgcgaacga
     5221 aaagcgaacc ggacagcgcc gatgcctgga ccgcgctcgg cgcggcgcgt ttcgacatga
     5281 gcgattttgc cggcgccgtt gcggcttacc agaaagccgt gaccctcgcg cccgaatcgg
     5341 cgggcctttg gtcggctctg ggcgaagcgc gcgtgatggg aagcgacggc gagccgatgc
     5401 ccgccgcggc ttcgcaggcg ttcgacaagg caatcgcgct cgatcccaaa gatcctcgcg
     5461 cgcgctattt cctcgcggtg agaaaggata tcggcggcga ccacaagggt gcgatcgagg
     5521 actggttcgc gctgctcgcc gacaccccgc aaggcgcgcc gtgggaggcc gatctgcgcc
     5581 gcacgatcga gcaggtcggg cagatccaca agatcgacgt gaccgcgcgg ctcgccgcga
     5641 cgcaggcgcg cccgttgaca cccaatgaaa tgccggtggc agcccgtgcg ataccagggc
     5701 cgagccgcgc cgacatggag gcggcgtcgc agcttcccca agggcagcag gaccagatga
     5761 tcgagggtat ggtgagcggg ctcgaagcca agctcaaggc caaccccgcc gacgtcgacc
     5821 gctggatcat gctgatgcgc agccggatga cgctcggc
//
LOCUS       DATMSU010000465        14105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_112017,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000465 DATMSU010000000
VERSION     DATMSU010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112017"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VN034_12410"
     CDS             complement(<1..1308)
                     /locus_tag="VN034_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 6 family protein"
                     /protein_id="HWV61456.1"
                     /translation="MLASTAHPALARDLLVADQAQYKAAVKKAQPGDTIILADGEWRD
                     FQIVFTGTGTPAKPIALTAETKGKVLITGQSNLRIGGKHLVVSGLVFKNGASPTDQVI
                     SFRRDSKTLASDSRVTETVIDGFSKPDRRAEDIWVALYGTGNRVDHSHFEGKTNAGVT
                     LAVIRRAGDPLDNRHRIDHNYFGPRPPLGSNGGETIRIGTSEESLSDSHTVVERNIFD
                     RTSGEVEIVSVKSGGNIVRENLVLASQGAFVLRHGNGNLVERNIFFGKGVPDTGGVRI
                     INRDQIVRDNYFEGLAGSSFKSAITVMNGVPNSVINRYHQVANARIEGNSLIDVARIT
                     LAAGADAERSAPPVDSKFERNLIVGAKGADPFRAEGEIGGIAFAGNVQAKVAKPLLTA
                     GVEQREVALERAANGLLYPTDPALAAVGAPRDLKPVTREEVGAS"
     gene            1486..4527
                     /locus_tag="VN034_12415"
     CDS             1486..4527
                     /locus_tag="VN034_12415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61457.1"
                     /translation="MIRDSLVRVSRGRMNFAAALLGTTALAMLAFPAFAQDTAAQDTA
                     PADEAGDDAIVVTGIRSSLATALGEKRTSDNIVEVIQAEDIGKLPDQNLAEVLENVTG
                     VQITRQAGVGNAVQIRGTDANRTEINGVSTIGSGAGRSGISFEDLPAALIASVEVTKV
                     PTAQTIEGSVGGTINLRTIRPLDLKEPLIAARAQMENSDLSKSTLPRLSATLGNRWDT
                     GIGEIGIVVSGSYARQDVAAFKPRVDRDAVVLPGSGPSAEAFPFLRIQFLQQGLENYE
                     YETYNGTAALEWKPNDDLKFYFDATLNNQTRGQQSHRVQISGTATPSVVDATDNSAFE
                     TVNWGTLDGPDGPIDLGSVDATVAGTLGIGGTTGTVIDPNMRTSSDTGARVTKSRVFD
                     LGSEWQVTDKLRMRAEVALSTSKSDFPNFSTTLDFINPNGPQPQIGRSIDNGVPIEFD
                     LRGGTLQFGIDQSSPFAPTTAQLLDPANYQLQQVAQGANSTDNQERAARLDFSYDTTD
                     LNPFVTSVDFGYRWNRTSAENNEFSRNVSLTNTTNAWNRPSGDLFSDILIPGPSNFNA
                     ADGRRLYFPDFLLIDGGLAFDDPAKVLDALNAAIDASNAAATEGPPVAPLTTPTESFA
                     GFFKIKETTNAAYFQANMEGEIAGMPVRGNAGVRWLHTSLSSTGNNIANGEVTGQTVA
                     KSSYNFLLPRFNLVLEPADRFLLRAGIARDIRRPNFDTLSTSFSFGTGANTPVAVGNP
                     DLVPEAVWSFDLSGEYYFAPSSLISIGFFHKSRTNLFAQRQEDPAPNLDADGNLNISI
                     DPSCPGGGIYNPIANRNINNPIQGVGICVPRSSTFNVPGTTTQTGVEVAFQHDLSAWE
                     DVLGFASGFGFIGNFTYQKAGGSAREYRTADGPRTVFTQLGYPGSQDLISLTNLSKYA
                     YNATLFYDKYGLNARVRYTWRSSYVSDDPFFFGLPLINGARGQLNASINYDINDHINV
                     GVEGINLLRGDQNQYCVNNKALLCFQGLTDRRITAGLSVKF"
     gene            complement(4611..9980)
                     /locus_tag="VN034_12420"
     CDS             complement(4611..9980)
                     /locus_tag="VN034_12420"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HWV61458.1"
                     /translation="MSRISKSRLLATSAIVGLSIGPIAAPVAAQDSFGIHNDQPELLE
                     IVIEEEDADVEGLDIGIYADNGPVTVDNAGEIRGLGTSIGSAQSRPSGGIVIAQPGSS
                     VTNRNSGEITGGSNGIATSYFFSEDANGDELPPQPLAANTSVTNAGTIIGQAGTGVGL
                     AGGGTVTNSGTIQGLTGNAGNGAQGIGVAISEFPDRIAEGVTGVGTITNSETGLIEGT
                     VFGALLQGGGTIDNAGIIRSTGNPNPATPGVSPFGVILTASPAQTGRTATVNNSGTIS
                     GFLGILAGGSLDAAVINNSGTINAPTTAIFANLTGGLVVNNAEGGEIISNGFITINST
                     GGTLTLDNAGLVRSNANTAINIRTAGASITNSGLIAGGGNDGIYANADGVTTINNAED
                     GVINGAASGIHAEFGGLNLTNAGSINGDGANPGFDAPPDAGVTISGGASSVTNSGSIT
                     GNRFGITTANYFNPATGQLQGLATGTTVVNSGTIYGYSDDGVRLIGGGSVTNSGEIGG
                     QIDAFADGVSMFAYTDQANEDYSALVTNEAEGKIAGQRYGVILSGGGDVVNAGELLGG
                     AGGLYIQGTALNNDPNEERSGLTASVVNSGTIRGLSDFGGTYTNGYGVGFGSDMSTAT
                     LVNSGTIGSEFAEGVSHGSLADLTVTNEEGGVITGATSGIYSGAGGTLIVDNAGIIRG
                     DGAYDGFDAPPDAGITIGTASSSVTNSGTISGAGAGITTAYLFDEEINQLVLLAVGTT
                     VENSGTIAGESNDGVRLIGGGSVTNSGAISGSGGEFADGISMFRGEGQTAEGYAATVA
                     NGADGTIDGARFGIILSGGGSVDNAGAIGGELGGVVIQSQFDGEDAGLTGTLTNSGTI
                     NSAADVGVQFNSGLASVVVDNSGAIDGATMGLVHGTDGTMALTNSGTIAGGVTGVESN
                     AGGSVTLVNSGTISGETGAAFTSLTQTSLDNSGILSGGSGVAVQLGAFDDGVILRTGS
                     AITGVVDAGDGTDTLTLDGDVLELTEAQQLTAANGFEALDVAAGYWSTSGFVGEFGNV
                     TVDAGASLQVNEVDLGEEGTSSPILTSTVRTDGLLVLNFGEDETVSALDDLTIDGAGQ
                     LQLIGEAVFTVDTANIAHTGGTIVSNGGLVLTGVLQGDVRTEGDGYFELGAGGTEGSF
                     AGNIVNDGRFVFNRSDDYDFLGDFSGNGVLDKKGDGALTFMGDYAFEGVTNILGGSVR
                     IGGTIDPTTDFNLGQGGTLDITGKDQTIGGLEGEEGSNVELGDSELTVDQPGNTAFGG
                     DISGTGSFVKQGEGTLNLTGNSTYTGPTSVNGGTLAVNGSIASPVTVNSGGTLGGNGS
                     VGSTTVAGGGTIAPGNSIGRLTVNGDLAFAPGSIYEVEVNAAGEGDRLDATGAVTIDS
                     TASVAVLAEDGEYNGRTDYVILTGAGGITGTFGTVTSDLAFLDPLLRYDANSVILSLY
                     RNDIDFADIAIGFNQASVATAVQALGVDNPLYEAVLVQNAAAAQASFGDLSGEILASA
                     MSGLTDDSRHLRNSLMGMKAPEESGAFVWGSAFGGWGDFDANRGNFAMDTDNKGLVAG
                     VGFGGNGFAAALSAGIGSSDFRFDGRNDRAEVDSKYLAAHASYGSGEGLRGTVGVSYA
                     WHDIDTSRGISGAPLAQTLTSKRDADTMQIFGEIGYDIVTGNMAVTPFARLAHVDTGS
                     DAFAETGGNAALAVGKADQKTTFLSLGARAQFNAGQPGFQPYVSAAWNRAMGDRGAPI
                     VSRFVAGGPAFDIVGIAIPKNSAEVEAGFEFTTGAFRLGAAYTGTLASDRSAHGARVT
                     ARIAF"
     gene            10342..12588
                     /locus_tag="VN034_12425"
     CDS             10342..12588
                     /locus_tag="VN034_12425"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV61459.1"
                     /translation="MRKLLAILIFTLLGLVQPAWAAPDTLPVLELHAHKDAPNLAPFI
                     RFAKRPGDTRTPQLDAILQGPLQQVEGSTIHFGPPGTRTVVLLQVRNASNEQGSWILT
                     TGRGSLKHFRLYEVKGDRLVLVVDGTDPQAARQNLGTYQAFSSELVLGPRQGTLVAIE
                     FISENSTYMPLKINSYGTFFKERRANISMVSGVVVGALTLLLLNFLFFSITGFREFLW
                     LAVAEAFFALNTVHSEGYITIFFLYDKPLAGLAVEDFIKCGFAASMAQFCRVFVKTPA
                     HFPRRDMVLKALIWSAVAIMLLQPGLAYYPPELRFLLHIATWLVTIGVALFLPFVGYA
                     AMKQLGFQLWPLFVGWASLALFIVYAAIASMGIFAWLPINWHLAGPVGLFEAVMVTLA
                     LGLNLKKIQRDKVIADWKYAQSLTERLEISERATRLAEEKAFALETVNSQNALLHASG
                     HDSRQVILALNSAVEVLRRQNRTDADDELVEMLESSADYLNEIVSTTISGANIAASDS
                     GFVALSSFRAEALVEPLSMMFKTPFAGKKLTLDVRVVDKVTIISDRPLLMRALANLLS
                     NSYKYTDSGGASIMLALEDGCAVITIRDSGGGMATNLVDALNAGTATRIRSDEKDQGT
                     GSGFGSAQRIIDTLQGSMKIVASSAAGTEIRITLPAAFASVTPVSADELRSHLDGWTL
                     LDFDDRAAFEGGLSAGAATGRTIALTYDDTTVTRGRLSQLVAMMAIKPACREMALHPL
                     FGERSEQV"
     gene            complement(12574..13191)
                     /locus_tag="VN034_12430"
     CDS             complement(12574..13191)
                     /locus_tag="VN034_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV61460.1"
                     /translation="MLAKHNILIVDDHRITQSGLRFLFGSLDRYTVVGALDRGATVNA
                     FVQSQPVDLVILDLNLPDVRGINVLAEIVGSRDMTVIILTGETHVNEIHSALKLGARA
                     IVSKSDSLDHIVAACDAALAGEIYVSPHIGEALGKFQQPPVALSSRQMAILHYLAQGE
                     TNKEIAYRLAIAPPTVSFHIAELRRKLDVSHNRKIVERANELNLL"
     gene            complement(13353..>14105)
                     /locus_tag="VN034_12435"
     CDS             complement(13353..>14105)
                     /locus_tag="VN034_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61461.1"
                     /translation="GAGAVAAVTGHPEFMALGLRCQPGGGLYMSVVPTKALHLPPAGR
                     PLLIGFRSHAEFTETTQQLMLEPGKPDFSGTLDPATQALLTGADSDLAVYASTDGGDE
                     WHEITYLSLAGSTAAIRSLERQCPQAAASPAAKPGQSRAVPLPVGYYVDMRIGTCAKP
                     FADGTLYLAEDRLISPYNSCKFTSLKMVDGEQFQQAESCPDEDGRPTAGTTNYRITGP
                     RSFAVETYSENWTWCPAAQLPAKARFYKGAGR"
BASE COUNT         2359 a         4614 c         4552 g         2580 t
ORIGIN      
        1 gctggcgccg acttcttcgc gggtgacggg tttgaggtcg cgcggcgcgc cgacggccgc
       61 cagcgccggg tcggtcggat agagcaagcc attggcggcg cgctcaagcg cgacctcgcg
      121 ctgctcgacc ccggcggtca gcaagggctt cgcgaccttg gcctgaacat tgcccgcgaa
      181 cgcgatgccg ccgatttcgc cctcggcgcg gaacgggtcc gcacccttcg cgccgacgat
      241 cagattgcgc tcgaacttgc tgtccacggg cggtgccgaa cgttcggcgt cggcgcccgc
      301 cgcaagggtg atgcgcgcga cgtcgatcag gctgttgccc tcgatccgcg cgttcgcgac
      361 ctgatggtag cggttgatca ccgaattggg cacgccattc atcacggtga tcgcgctctt
      421 gaacgagctg cccgcgagcc cttcgaaata attgtcgcgc acgatctggt cgcggttgat
      481 gatccgcacg ccgccggtgt cgggcacgcc cttgccgaag aagatgttgc gttcgacgag
      541 gttgccgttg ccgtggcgga gcacgaaggc gccctgcgac gccagcacca gattttcgcg
      601 gacgatattg ccgcccgact tgaccgagac gatttcgacc tcgccgctcg tccggtcgaa
      661 gatgttgcgt tcgacgaccg tgtggctgtc ggacagcgat tcctcgctgg tgccgatgcg
      721 gatcgtctcg ccgccgttcg atccgagcgg cgggcgcggg ccgaaataat tatgatcgat
      781 gcggtggcgg ttatcgagcg gatcgcccgc gcggcggatg accgcgagcg tcacgccggc
      841 gttggtcttg ccttcgaaat gcgaatgatc gacgcggttg ccggtaccat agagcgcgac
      901 ccagatatcc tcggcgcggc gatccggctt gctgaagccg tcgatcacgg tctcggtgac
      961 gcggctgtcg ctcgcgagcg ttttggaatc gcggcggaag ctgatcacct gatcggtcgg
     1021 gctggcgccg ttcttgaaga cgaggcccga gacgacgaga tgctttccgc cgatgcgcag
     1081 gttcgactgg ccggtgatga gcaccttgcc cttggtctca gcggtcagcg cgatcggttt
     1141 ggcgggggtg ccggtgccgg tgaagacgat ctggaaatcg cgccattcgc cgtcggcgag
     1201 gatgatcgtg tcgcccggct gcgccttttt caccgcagcc ttatattgcg cctgatcggc
     1261 cacgagcagg tcgcgcgcca gcgcaggatg cgccgtcgat gccagcatcg ctaccgttag
     1321 aatcgccctt atctgcaccg cgcctcgccc tttttatgat tctcgcctat tggactatac
     1381 aagaggtagg acaatatcaa cccgcttatg gtctgaatca tgggttgaca tattggtgtg
     1441 acaagaggag cttctctcca aacaagggca aacagggaga gtgggatgat tcgggatagc
     1501 ctcgtgcgcg tttcgcgcgg cagaatgaat tttgcagcgg cgctgttggg cacgacggcg
     1561 ctggcgatgc tcgccttccc cgccttcgcg caggacacgg ccgcacagga tacggcccct
     1621 gccgacgagg cgggcgatga cgcgatcgtc gtcacgggca tccgcagctc ccttgccacc
     1681 gcgctgggcg aaaagcgcac ctccgacaat atcgtcgagg tcatccaggc cgaagatatc
     1741 ggcaaactgc ccgaccagaa tttggccgaa gtgctggaga atgtcaccgg ggtccagatc
     1801 acgcggcaag cgggcgtcgg caacgcggtg cagatccgcg gcaccgatgc caaccggacc
     1861 gaaatcaacg gcgtatcgac gatcggatcg ggcgccggcc ggtcgggcat cagtttcgag
     1921 gatctgcccg cggcgctgat cgcgtcggtc gaggtgacca aggtcccgac cgcgcagacg
     1981 atcgaggggt cggtcggcgg cacgatcaac ctgcggacga tccgcccgct cgacctcaag
     2041 gaaccgctga tcgccgcgcg cgcgcaaatg gaaaacagcg acctgtcgaa atcgaccctg
     2101 ccgcggctgt ccgccacgct cggcaaccgg tgggacaccg gcatcggtga aatcggcatc
     2161 gtggtgagcg ggagctatgc ccggcaggac gttgccgcgt tcaagccgcg cgtcgaccgc
     2221 gacgcggtcg tcctccccgg atcgggcccg agcgccgagg ccttcccctt tctgcgcatc
     2281 cagttccttc agcagggatt ggagaattac gaatatgaaa cctacaacgg caccgccgcg
     2341 ctggaatgga agccgaacga cgacctgaaa ttctatttcg acgcgacgct caacaaccag
     2401 acgcgcgggc agcaaagcca ccgtgtgcag atttcgggca cggcgacgcc atcggtcgtc
     2461 gacgcgaccg acaatagcgc gttcgagacg gtcaactggg gcacactcga cggtcccgac
     2521 ggtccgatcg atctcggcag cgtcgatgcc actgttgccg gcaccctcgg catcggcgga
     2581 acgaccggaa cggtgatcga tccgaatatg cgcacgtcga gcgataccgg cgcgcgcgtc
     2641 accaagagcc gggtgttcga ccttggcagc gaatggcagg tgaccgacaa gctgcggatg
     2701 cgcgccgaag tggcgctgtc gacctcgaaa tcggactttc cgaacttctc gacgacgctc
     2761 gatttcatca atccgaatgg cccgcagccg caaatcgggc gcagcatcga caatggcgta
     2821 ccgatcgaat tcgatctgcg cggcggcacg ctgcagttcg gcatcgacca gtcgagcccc
     2881 ttcgcgccga cgacggcgca attgctcgat cccgccaatt accagctgca gcaagtggcc
     2941 cagggcgcca attcgaccga caatcaggaa cgggcggcgc gcctcgattt ctcctatgac
     3001 acgaccgacc tcaacccctt cgtcacctcg gtcgatttcg gttatcgctg gaaccggacc
     3061 tcggcggaga acaacgaatt ttcacgaaat gtcagcctga ccaacacgac gaatgcgtgg
     3121 aaccggccgt cgggcgacct gttttccgac atattgatcc cggggccgag caacttcaac
     3181 gcggccgacg ggcgccggct ctatttcccc gacttcctgc tgatcgacgg cgggctggca
     3241 ttcgacgatc cggcgaaggt gctcgacgcg ctcaacgccg cgatcgacgc gagcaacgcc
     3301 gccgcgaccg agggaccgcc cgtcgcgccg ctgacgacgc cgaccgaatc cttcgcgggt
     3361 ttcttcaaga tcaaggaaac gaccaacgcc gcctatttcc aggcgaatat ggagggcgaa
     3421 atcgccggca tgccggtgcg cggcaacgcc ggcgtgcgct ggctgcatac cagcctgtcc
     3481 tcgaccggca acaatatcgc caacggcgaa gtcacggggc agaccgtcgc caaaagctcg
     3541 tataatttcc tgttgccgcg cttcaacctc gtgctcgaac ccgccgaccg cttcctcctc
     3601 cgcgccggta tcgcgcgcga cattcgccgc ccgaatttcg acacgctgtc gacctccttc
     3661 tccttcggca ccggcgcgaa cacgccggtc gcggtgggca atcccgacct cgtccccgaa
     3721 gccgtgtggt cgttcgacct gtcgggcgaa tattatttcg cgccgtcgag cctgatcagc
     3781 atcggattct tccacaagag ccgcaccaat ttgttcgcgc agcggcagga agatccggcg
     3841 cccaatctcg acgccgacgg caacctcaac atttcgatcg acccgtcatg ccccggcggc
     3901 ggcatctata atccgatcgc caaccgcaac atcaacaacc cgattcaggg cgtcggcatc
     3961 tgcgtcccgc gcagttcgac cttcaacgtt ccgggcacga caacgcaaac cggcgtcgaa
     4021 gtcgcctttc agcacgatct gtcggcgtgg gaggatgtgc tcggcttcgc ctcgggcttc
     4081 ggcttcatcg gcaatttcac ctatcaaaag gcgggcggct cggcgcgcga atatcgcacc
     4141 gcagacgggc cgcggacggt gttcacccag ctcggctatc cgggctcgca agacctgatc
     4201 tcgctgacca acctgtcgaa atatgcctat aatgcgacgc tgttctacga caaatacggg
     4261 ctcaacgcgc gcgtgcgcta tacgtggcgg tcgagctatg tgtcggacga tcccttcttc
     4321 tttggcctgc cgctgatcaa cggcgcgcgg ggccagctca acgccagcat caattacgat
     4381 atcaacgatc atatcaacgt cggcgtcgag ggcatcaacc tgctgcgcgg cgaccagaac
     4441 caatattgcg tgaacaacaa ggcgctgctc tgcttccagg gcctgaccga ccgccggatt
     4501 accgcgggat tgagcgtcaa attctgacga gcccgacaaa aggatgaggc cggggcaaca
     4561 taggttgcct cggcctcagg ctacgcgagc cgcttccgac cgggtcgctg tcagaaggcg
     4621 atgcgcgcag tcacccgtgc accatgggcg ctgcgatccg acgccagcgt gccggtgtag
     4681 gccgcgccga ggcggaaagc ccccgtggtg aattcaaagc cggcttcgac ctccgccgaa
     4741 tttttgggga tcgcgatgcc gacgatgtcg aacgccggtc cgcccgcgac gaagcgcgag
     4801 acgatcggag cgccgcggtc gcccatcgcg cggttccacg ccgccgagac atagggctgg
     4861 aagcccggct gtcccgcgtt gaactgcgcc cgtgcgccga ggctgaggaa ggtcgtcttc
     4921 tggtcggcct tgcccaccgc cagcgccgca ttgccgccgg tttcggcgaa ggcgtcgctg
     4981 ccggtgtcga catgcgccag ccgggcgaag ggcgtgaccg ccatgttgcc ggtgacgata
     5041 tcataaccga tctcgccgaa gatctgcatc gtgtcggcgt cgcgcttcga cgtcagcgtc
     5101 tgggcgagcg gcgcgccgct gatgccgcgg ctggtatcga tgtcgtgcca ggcgtagctg
     5161 accccgacgg tgccgcgcag cccttcgccg ctgccatagc tggcgtgcgc ggcgagatat
     5221 ttgctgtcga cctcggcgcg gtcattgcga ccatcgaaac ggaagtccga actgccgatc
     5281 cccgccgaaa gcgccgcggc gaagccgttg ccgccgaagc cgacgccggc gaccagcccc
     5341 ttgttgtcgg tatccatcgc gaaattgccg cgattggcgt cgaaatcgcc ccagccgcca
     5401 aaggccgagc cccagacgaa tgcgccgctt tcctcaggcg ccttcatgcc catgaggctg
     5461 ttccgcagat gccggctgtc gtcggtcagc ccgctcatcg cgctcgccag gatttcgccc
     5521 gacaggtcgc cgaagctggc ctgcgccgcc gccgcattct gcaccagcac cgcttcgtaa
     5581 agcgggttgt cgacgccgag cgcctgcacc gcggtcgcga cgctcgcctg gttgaagccg
     5641 atcgcgatgt cggcgaaatc gatgtcgttg cgatagagcg acaggatcac cgaattggcg
     5701 tcgtaacgca gcagcggatc gaggaaggcg agatcgctcg tcaccgtccc gaaggtgccg
     5761 gtgatgccgc ccgcgccggt caggatcaca taatcggtcc gcccgttata ttcgccgtct
     5821 tcggcgagca cggcgacgct cgccgtgctg tcgatggtga cggcgccggt ggcgtcgaga
     5881 cgatcgcctt cgccagcagc gttgacttcg acctcgtaga tcgagccggg ggcgaaggcg
     5941 agatcgccgt tcacggtcag ccggccgatc gaattgcccg gcgcgatcgt gccgccgccc
     6001 gcgaccgtgg tcgaaccgac gctgccgttg ccgccgaggg tgccgccgct gttgacggtg
     6061 accggcgagg cgatcgagcc gttcaccgcg agggtgccgc cgttgaccga ggtcgggccg
     6121 gtatatgtgc tgttaccggt caggttgagc gtgccctcgc cctgcttgac gaagctgccg
     6181 gtgcccgaga tgtcgccccc gaaggcggta ttcccgggct ggtcgacggt cagctcgctg
     6241 tcgccgagtt cgacgttcga gccttcctcg ccctcgaggc cgccgatcgt ctgatccttg
     6301 cccgtgatgt cgagcgtgcc gccctggccg agattgaaat cggtcgtcgg atcgatcgtc
     6361 ccgccgatgc gcaccgagcc gccgaggatg ttcgtcacgc cttcgaaggc atagtcgccc
     6421 atgaaggtca gcgcgccgtc gcccttcttg tcgagcacgc cattgcccga aaaatcgccg
     6481 aggaagtcat aatcgtccga gcggttgaag acgaagcggc cgtcgttgac gatattaccg
     6541 gcaaagctgc cctcggttcc gccggcgccg agctcgaaat agccgtcgcc ttcggtccgc
     6601 acgtcgccct gcagcacgcc cgtgagcacg agcccgccat tcgagacgat cgtgccgccg
     6661 gtatgcgcga tgttggcggt atcgacggtg aacacggcct cgccgatcag ctggagctgc
     6721 cccgccccgt cgatcgtcag atcgtcgagc gccgagacgg tttcatcctc gccgaagttg
     6781 agcacgagca agccgtcggt ccgcaccgtc gaggtgagga tcggcgacga ggtgccttcc
     6841 tcgcccagat cgacttcgtt gacctggagc gacgcgcccg catcgaccgt cacattgccg
     6901 aattcgccga cgaagcccga cgtcgaccaa tagcccgccg cgacgtcgag cgcctcgaag
     6961 ccgttggcgg cggtcagttg ctgcgcctcg gtcagttcga ggacgtcgcc gtcgagcgtc
     7021 agcgtgtcag tgccgtcgcc cgcgtcgacc acgccggtga tcgcgctgcc cgtgcgcaga
     7081 attacgccgt cgtcgaatgc gcccagctgc accgcgacgc ccgatccgcc cgacaggatg
     7141 cccgaattgt cgaggctggt ttgggtcagc gaggtgaaag ccgcgccggt ttcacccgaa
     7201 atcgtcccgc tgttcaccag cgtgaccgaa ccgcccgcgt tgctttccac gccggtcacc
     7261 ccgccggcga tcgtgccgct gttcgtcagc gccatcgtgc cgtcggtgcc gtggacgagg
     7321 cccatcgtcg cgccatcgat cgcgccgctg ttgtcgacga cgaccgatgc cagtccggaa
     7381 ttgaattgaa ccccgacgtc ggccgcgctg ttgatcgtgc cgctgttcgt cagcgtcccg
     7441 gtcagcccgg catcctcgcc gtcgaactgg ctctggatga cgacgccgcc cagctcgccg
     7501 ccgatcgccc ccgcgttatc gacgctgcct ccacccgaca ggatgatgcc gaagcgggcg
     7561 ccgtcgatcg tgccatcggc gccgttcgcc accgtcgccg cgtaaccttc ggcggtctgc
     7621 ccttcgccgc ggaacatcga aatgccgtcc gcgaactccc cgccgctgcc cgaaatcgca
     7681 ccgctgttgg tgacgctgcc gccgccgatc agccgcacgc cgtcgttcga ttcgccggcg
     7741 atcgtgccgc tgttttcgac ggtggtcccg acggcgagca ataccagctg gttgatctct
     7801 tcatcgaaga gataggcggt ggtgatgccc gcgcctgcgc cgctgatcgt gccgctgttg
     7861 gtcacgctcg acgacgcggt gccgatggtg atgcccgcgt cgggcggcgc gtcgaagccg
     7921 tcataggcgc cgtcgccacg aatgatgccc gcattgtcga cgatcagcgt gccgcccgcg
     7981 ccgctgtaga tgcccgaggt cgcgccggtg atcactccgc cttcctcgtt ggtgacggtc
     8041 aggtcggcga gcgaaccgtg gctgacgccc tcggcgaatt cgctgccgat cgtgccgctg
     8101 ttgaccagcg tcgcggtcga catgtcgctg ccgaagccga cgccatagcc gttggtatag
     8161 gtgccgccga agtcgctgag cccgcggatc gtgccgctgt tgacgacgct ggcggtcagc
     8221 ccgctgcgct cctcgttcgg gtcgttgttg agcgcggtgc cctggatata gaggccgccc
     8281 gcaccgccga gcagttcgcc ggcgttgacg acatcgccgc cgcccgaaag gatgacgcca
     8341 tagcgttgcc cggcgatctt gccttcggcc tcattggtga cgagcgcgct ataatcctcg
     8401 ttcgcctgat cggtgtaggc gaacatcgac acgccgtcgg caaaggcgtc gatctggccg
     8461 ccgatttcgc cgctgttggt gacgctgccg ccgccgatca gccgcacgcc atcgtcggaa
     8521 tagccataga tggtgccgct gttgaccacg gtggtgcccg tcgcgagccc ctggagctgg
     8581 ccggtcgcgg gattgaaata attggcggtg gtgatgccga agcggttgcc cgtgatgctg
     8641 ccgctgttcg ttaccgagct ggcgccgccc gaaatggtga cgcccgcgtc gggcggggcg
     8701 tcgaagccgg ggttggcgcc gtcgccgttg atcgagcccg cgttggtcag gttgagcccg
     8761 ccgaactcgg catggatgcc cgacgccgcg ccgttgatca cgccgtcttc ggcgttgttg
     8821 atcgtcgtga cgccgtcggc gttcgcataa atgccatcgt ttccgccacc ggcgatcaag
     8881 ccgctgttgg tgatcgaagc gcccgcggtg cggatgttga tcgcggtgtt ggcattgctg
     8941 cgcaccagcc cggcgttgtc gagcgtcaac gtcccaccgg tcgaattgat cgtgatgaag
     9001 ccgttcgata tgatctcgcc accctctgcg ttattgacga cgaggccgcc cgtcaggttg
     9061 gcgaagattg cggtcgtcgg ggcgttgatg gtgccgctgt tgttgatcac cgccgcatcg
     9121 agcgagccgc ccgcgagtat gccgaggaaa cccgaaatgg ttccgctgtt gttgacggta
     9181 gcggtgcgtc cggtctgagc gggcgaagct gtcaggatca cgccgaacgg gctgaccccc
     9241 ggcgtggcgg gattgggatt gccggtgctg cgaatgatac ccgcgttgtc gatcgtgccg
     9301 cccccctgaa gcagcgcgcc gaagaccgtc ccttcgatca ggccggtttc gctgttggtg
     9361 atggtgccga cgccggtcac gccctcggca atgcggtcgg ggaattcgga aatcgcgacg
     9421 ccgatgccct gcgcgccgtt gccggcattg ccggtgaggc cctggatcgt gccgctattg
     9481 gtaacggtgc cgccgccggc caggccgacg ccggtgcccg cttggccgat gatcgttccg
     9541 gcgttggtga cgctggtgtt cgccgcgagc ggctgcggcg gcagttcgtc gccgttcgcg
     9601 tcctcgctga agaaatagga ggtcgcgatg ccgttcgagc cgccggtgat ttcgccgctg
     9661 ttgcggttgg tgacgctcga tccgggttgc gcgataacga taccgccgct ggggcggctt
     9721 tgggcgctgc cgatgctggt gccgaggccg cgaatctcgc cggcattgtc gacggtgaca
     9781 gggccattgt ccgcataaat gccaatgtcg agcccctcga catcggcgtc ttcctcttcg
     9841 atcacaattt ccagcaattc gggctgatcg ttgtgaatgc cgaaggagtc ctgcgccgcc
     9901 acgggcgccg cgatcgggcc gatcgacagg ccgacgatcg cgctggtggc cagcaggcgg
     9961 gatttcgaaa tacgcgacat gatatgactc ccctcgacat tgcagatggc gaagggttcg
    10021 ccggaggacg ctttggtcct ccggctggtc acccccacct cgaaattgcg tcccccagct
    10081 gatcggatga ctggcatctt tcagctttgt gcgcgttgac tagggcgggg gtcccccgag
    10141 cgtctaccca atgttttttt gggtgaattt gcgacggacc gttgacgtca tgcttgcaca
    10201 acgggccgca cggccattac ggtgacccgt caccgggggg tgggtcgctg atattcgggg
    10261 gaaaacgtgg ggtgcgcgcg cctgcccccc cttgaacatg atggaaccgc cggccccgca
    10321 accgaacgac gggtgccaat gatgcgaaag ctgctcgcga tcctgatttt caccctgctc
    10381 gggttggtgc agccggcgtg ggccgcgccg gacacattgc ccgtgctcga actacatgcc
    10441 cataaggatg cgcccaatct ggcgcctttc atacgcttcg ccaagcggcc cggcgacact
    10501 cggacacctc agttggacgc catcctccag ggcccgcttc agcaggtgga gggttcgacc
    10561 atccacttcg ggccgccggg cacccgcacc gtcgttctgc tgcaggtccg caatgccagc
    10621 aacgagcaag gaagctggat cctgacgacg ggccgcgggt cgctcaaaca cttccgcctc
    10681 tatgaagtga aaggtgatcg cctcgtcctg gtggtcgatg gcaccgaccc gcaggccgcg
    10741 cggcaaaatc tcgggactta tcaggccttc agctccgaac tcgtcctcgg tccgcgtcag
    10801 ggcacgctgg tggccatcga gtttatttcg gaaaactcga cctatatgcc gctgaagatc
    10861 aacagctacg gcaccttctt caaggaacgg cgcgcgaata tttcgatggt gtcgggggtg
    10921 gtggtcggcg cgctgacgct gctgctcctc aacttcctct tcttctcgat cacgggtttc
    10981 agggaatttc tctggctggc ggtcgcggag gccttcttcg cgctgaacac ggtccattcc
    11041 gaaggctata tcacgatctt tttcctttac gacaaaccgc tggcggggct cgcggtcgag
    11101 gatttcatca aatgcggctt tgccgcgtcg atggcgcaat tctgccgggt cttcgtcaag
    11161 accccggccc attttccgcg gcgcgacatg gtgctcaagg cgctgatctg gtccgcggtc
    11221 gcgatcatgc tgctccagcc gggcctcgcc tattacccgc ccgagctgcg cttcctgctg
    11281 cacatcgcga catggctggt cacgatcggg gtcgcgttgt tcctgccctt cgtcggctat
    11341 gccgcgatga agcagctcgg cttccagctc tggccgctgt tcgtcggctg ggcgagcctt
    11401 gcgctcttca tcgtctatgc ggcgatcgcg tcgatgggca ttttcgcatg gctgcccatc
    11461 aactggcacc tcgccggacc ggtcggtttg ttcgaagcgg tgatggtgac gctcgcgctg
    11521 gggctgaacc tcaagaagat ccagcgcgac aaggtgatcg ccgactggaa atatgcgcag
    11581 tcgctgaccg agaggctgga aatcagcgag cgcgcgacgc ggcttgccga ggaaaaggcc
    11641 ttcgcgctcg aaacggtcaa cagccagaat gcgttgcttc acgcatcggg gcacgacagc
    11701 cggcaggtga tcctggcgct caacagcgcg gtcgaggtgc tcaggcgcca gaaccggacc
    11761 gatgccgacg acgagctggt cgaaatgctc gaaagctcgg ccgattatct caatgagatc
    11821 gtgtcgacga cgatttccgg cgccaatatc gcggcgagcg attcgggctt tgtcgcgctc
    11881 agcagctttc gcgccgaggc gctcgtcgaa cccttgtcga tgatgttcaa aacccccttc
    11941 gcgggtaaga agctgacgct cgacgtccgc gtcgtggaca aggtaacgat catttccgac
    12001 cggcccttgc tgatgcgcgc gctcgccaac ctgctcagca acagctacaa atataccgat
    12061 agcgggggcg caagcatcat gctcgcgctc gaagacgggt gcgccgtcat cacgatccgc
    12121 gacagcggcg gcggcatggc gaccaacctc gtcgacgcgc tcaacgccgg caccgcgacc
    12181 cggatccgct ccgacgagaa ggaccagggc acgggatcgg gtttcgggtc ggcgcagcgc
    12241 atcatcgaca cgctgcaagg gtcgatgaag atcgtcgcgt cgagcgccgc ggggaccgaa
    12301 atccggatca cgctgccggc ggcctttgca tcggtcacgc cggtatccgc cgacgaattg
    12361 cggtcgcatc tcgatggctg gacgctgctc gatttcgatg accgcgccgc gttcgaaggc
    12421 gggttgagcg ccggcgccgc gaccggccgg acgatcgcgc tcacctatga cgacacgacg
    12481 gtgacgcgcg ggcggctcag ccagctggtc gcgatgatgg cgatcaagcc cgcgtgccgc
    12541 gaaatggcgc tgcacccgct tttcggcgaa cgatcagagc aggtttagct cgtttgcgcg
    12601 ctcgacgatc ttgcgattgt gcgagacgtc gagcttgcgc cgcagttcgg cgatatggaa
    12661 ggacacggtc ggcggcgcga tcgccagccg ataggcgatt tccttgttcg tctcgccctg
    12721 cgccagatag tgaagaatcg ccatttgccg cgacgacagc gcgaccggcg gctgctgaaa
    12781 cttgccgagt gcctcgccga tatgcggcga aacatagatt tcgccggcga gcgccgcgtc
    12841 gcaggcggcg acgatatggt cgagcgagtc cgacttgctg acgatcgcgc gcgcgccgag
    12901 tttcagcgcc gaatgaatct cgttcacatg cgtttcgccg gtcaggatga tgaccgtcat
    12961 gtcgcgcgac cccacgattt cggccagcac gttgatcccg cgcacgtcgg gcaggttgag
    13021 gtcgaggatc acgaggtcga cgggctgcga ctggacgaag gcgttgacgg tcgcgccgcg
    13081 atcgagcgcc ccgacgaccg tatagcggtc gagcgagccg aacaggaacc gcagtccgct
    13141 ctgggtgatg cggtgatcgt cgacgatcag gatattatgc tttgccaaca tgcccacgcc
    13201 ccgcctgccc gatacggatt gatcagcaga ttttcccgtc gccgcaaccc gaataatatt
    13261 ggggggcgcc ggacgcgacg atgatcggga tgtcacggcc gccggctccg gcgatgccgg
    13321 acgggcatgg ttgcgcgcgc cgccccggct gatcaacgcc ccgccccctt gtagaagcgc
    13381 gccttcgccg gcagttgcgc ggcgggacac caggtccagt tttcgctata ggtttcgacc
    13441 gcgaaggacc gcggcccggt gatgcgatag ttggttgtcc cggcggtcgg ccgcccgtcc
    13501 tcgtccggac agctctcggc ttgctggaac tgttcgccat cgaccatctt gaggctggtg
    13561 aacttgcagc tgttgtatgg cgatatgagg cgatcttcgg cgagatagag cgtcccgtcg
    13621 gcgaaaggtt tggcgcaggt gccaatgcgc atatcgacat aatagccgac gggcagcggc
    13681 acggcccgcg attgcccggg tttcgccgcg ggcgaggcgg ccgcctgcgg acattgccgt
    13741 tcgagcgagc ggatcgctgc ggtcgacccg gcgagcgaca gataggtgat ctcgtgccac
    13801 tcgtcgccgc cgtcggtgct ggcatagacg gcaaggtcgc tgtcggcgcc ggtcagcagc
    13861 gcctgtgtcg cggggtcgag cgtgccgctg aaatcgggct tgcccggctc gagcatcaac
    13921 tgctgcgtcg tttcggtaaa ctccgcgtgg ctcctgaagc cgatcaggag cggccggccc
    13981 gcgggcggca gatgcaacgc cttcgtcggc acgacgctca tatagaggcc gccgcccggc
    14041 tggcagcgca gccccaaggc catgaattcg ggatgccccg tcaccgccgc gaccgcgccg
    14101 gcgcc
//
LOCUS       DATMSU010000466         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_121888,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000466 DATMSU010000000
VERSION     DATMSU010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121888"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VN034_12440"
     CDS             complement(<1..431)
                     /locus_tag="VN034_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWV61462.1"
                     /translation="MPDFMQTPLDRSYRADNARKLPISANHGNPKTESAELPASAFKA
                     MLRGARGRCPRCNEAKLFHRFLKPVLICSACAQDWTHQQADDFPAYIAILLTGHIMAP
                     IIIALVQDTKLSLIALAAIIVTAMLVLMIGFLQPAKGAIIA"
     gene            581..901
                     /locus_tag="VN034_12445"
     CDS             581..901
                     /locus_tag="VN034_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV61463.1"
                     /translation="MITSQQMRAARALLGIDQRELAELAGLSLPTIQRMEASNGQVRG
                     VVDTLVKVITALESAGIELLGEYSSSVGSGRGVRLREPADADPKAPGRALLSAKFPSR
                     PAIR"
     gene            898..>2483
                     /gene="sulP"
                     /locus_tag="VN034_12450"
     CDS             898..>2483
                     /gene="sulP"
                     /locus_tag="VN034_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020818230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HWV61464.1"
                     /translation="MKSEAYTPKLFTVFREGYSVATFRADAIAGLTVAIVALPLAMAL
                     GIASGASPDKGLITAVVAGFLISALGGSRVQVGGPTGAFVVVIFNVIASHGYDGLLIA
                     TLLAGLILIAAGLLRFGQMIKYIPHPVVTGFTAGIAVIIASSQVKDFFGLAIDKVPAD
                     FLPKWQAYLGALSSANWAAIGVGGGALAIIIAFRKFAPRLPGFLIAVVVSSLAVALLN
                     LPVDTIGSRFPDIPAGLPLPSFPDISLVKINAVLPSAFTIAFLAGIEALLSAVVADGM
                     AGTRHRSNQELVGQGVANLGSALFGGLPATGAIARTATNIRSGAKTPVAGMMHAAFLL
                     VFILFGTDLMAYVPMAALAAILFMVAWGMSEYQRFLALLRMPNSDRAVLLITFGLTVL
                     VDLTVAIAVGVTLASLLFMARMAEAVEVDRSGRRDAELDAEDLDQRDDLPPGVEVFRI
                     AGPFFFGVAGELLDTLRRVGQSPRVIILRMRLVPLLDASGAQALEEFVEQARLAGARV
                     IVSGVQPQPRAMLGRIGLGEA"
BASE COUNT          401 a          772 c          811 g          499 t
ORIGIN      
        1 aatgcgatta tcgcgccctt ggcgggttga aggaacccga tcatcaaaac gagcatggcg
       61 gtcacgatta tcgcggccaa tgcgatgagc gacagcttcg tgtcctgaac gagtgcaatg
      121 atgatcggcg ccataatatg ccccgtcagg aggatcgcga tataagccgg gaaatcgtcg
      181 gcttgctgat gcgtccagtc ttgcgcgcac gcggagcaaa taaggacggg tttgagaaag
      241 cgatgaaata gcttcgcttc gttgcagcgg ggacagcgac cgcgcgcgcc gcggagcatc
      301 gctttgaagg ccgaggcagg aagctccgcg ctttccgtct taggattccc gtgattggcg
      361 gagatcggta attttcgggc gttgtccgca cgataagagc gatccagagg cgtttgcatg
      421 aaatcgggca tcagccgcct cctgcgcgaa ggtgggcgca gcgagcggct cccgccaaag
      481 caagcatatt cgatatagca gagagcatga tgtatatata cagcattgat ccataatgtt
      541 aagattgata aatgatgtat gaaacatcat tggagtcgat atgatcacat cgcagcagat
      601 gcgcgcggcg cgcgcgctcc tcgggatcga ccagcgcgaa cttgccgagc ttgccggtct
      661 ctcgcttccg acgatccagc gcatggaagc gtcgaacggt caggtgcgcg gtgtcgtcga
      721 tacgctggtg aaggtgatta ccgcgcttga aagcgcaggc atcgaactgc tcggcgaata
      781 ttcatcgagt gtcggatccg ggcgcggcgt gcggcttcgc gagcccgccg acgccgatcc
      841 caaggcgccc ggccgcgcgc ttctgtccgc caaatttccg agccggcccg caatccggtg
      901 aagtccgagg cctatacgcc gaagctcttc acggtctttc gcgagggtta ttccgtcgcc
      961 acatttcggg ccgatgcgat cgcgggtctg acagttgcca tcgtcgcgtt gccgcttgcc
     1021 atggcactcg gtattgccag cggcgcctcg cccgacaagg ggctgatcac agccgtcgtc
     1081 gcgggtttcc tgatctcggc gctcggcgga tcgcgcgtgc aggtgggcgg gccgaccggc
     1141 gccttcgtcg tcgtgatctt caacgtcatc gcgagccacg gctatgacgg cctgttgatc
     1201 gcgaccctgc tcgccgggct catcctcatc gcggcgggac tgctgcgctt cggccagatg
     1261 atcaaatata tcccgcatcc ggtggtgacc ggcttcaccg ccgggatcgc ggtcatcatc
     1321 gcctcgagtc aggtcaagga tttcttcggc ctcgccatcg acaaggtgcc cgccgatttc
     1381 ctccccaagt ggcaggctta tctgggggcg ctgtccagcg cgaactgggc cgcaatcggc
     1441 gtcgggggcg gcgcgctggc aatcatcatc gcgtttcgca aattcgcgcc gcgcctgccg
     1501 ggcttcctga tcgcggtggt ggtaagctca ctcgcggtgg cacttttgaa cctgccggtc
     1561 gacacgatcg gatcgcgctt tcccgacatt ccggcgggcc ttccgttgcc ctcgtttccc
     1621 gacatctcgc tcgtcaagat caatgccgtc ctgccgtcgg ccttcacgat cgcctttctc
     1681 gccgggatcg aggcgctgct gtccgcagtc gtcgccgacg gcatggccgg tacgcggcat
     1741 cgttcgaacc aggaactggt cgggcagggc gtcgccaacc tcggttcggc gctgttcggc
     1801 ggtctccccg ccaccggggc gatcgcgcgc accgcgacca atatccggtc gggtgcgaag
     1861 acgcccgtcg cgggcatgat gcacgcggcc tttctgctcg tcttcatcct cttcggcacg
     1921 gacctgatgg cctatgtgcc gatggcggcg ctcgccgcga tcctgttcat ggtcgcctgg
     1981 ggcatgagcg agtatcagcg cttcctcgcg ctgctgcgga tgccgaacag cgaccgggcg
     2041 gtgctgctca tcaccttcgg attgacggtc ctcgtcgatc tgacggtggc gatcgcggtc
     2101 ggggtgacgc tcgcgtcgct gcttttcatg gcgcgtatgg ccgaagcggt cgaggtcgat
     2161 cgaagcgggc ggcgcgacgc cgaactcgat gccgaagatc tcgaccagcg cgatgacctg
     2221 ccgccgggcg tcgaggtgtt ccggatcgcc ggtcccttct ttttcggtgt cgcgggtgag
     2281 ctgctcgaca cgctgcgccg cgtcgggcag tcgccgcgcg tcatcatctt gcggatgcgg
     2341 ctcgtaccgc tcctcgatgc gagcggcgcg caggcacttg aggaatttgt cgaacaggcc
     2401 cggctggcgg gcgcgcgggt gatcgtctcg ggcgtgcagc cccagccgcg ggcgatgctc
     2461 gggcggatcg gccttggcga agc
//
LOCUS       DATMSU010000467         4833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_149653,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000467 DATMSU010000000
VERSION     DATMSU010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149653"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VN034_12455"
     CDS             complement(<1..300)
                     /locus_tag="VN034_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWV61465.1"
                     /translation="MIEHHGSCHCGRVHLVVRDEPTEVSECNCSICRRTAGLWHYCPP
                     KSVTVEGEGVAYQQGDRALDTWHCQACGCTTHWTPTDPNYQRMGVNLRMFEPDLWR"
     gene            complement(421..1953)
                     /locus_tag="VN034_12460"
     CDS             complement(421..1953)
                     /locus_tag="VN034_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWV61466.1"
                     /translation="MTSIEMLEGDFATLPDLVRAHAAERPDAVAAADPFRRLSWSELD
                     QLTDRIAARLQQDGFTKGDRTAIAGLNSVEQMAVILGTLRAGGIAGLVTNSATGEQTA
                     AMIADTGARHLFLDSAAKSSLDGQVIAASDLIAMDGSDAGAPLDAWLSLAGVKPAPVD
                     IRPEDGFNIIYSSGTTGTPKGIVHSHAMRWQHIIRGAPAYGPNAVTILSTPLYSNTTM
                     ASFMPTVGSGGQVVLMKKFDARGFLELAERERATNCMLVPVQYRRIMALDDFASFDLS
                     SFVMKYCTSAPFPAALKADVLERWPGGLVEIYGMTEGGAAFILEAHQFTDKLHTVGKP
                     APGHIAKVIDEEGKELPQGSVGEVVGRSPAMMTGYNNRPDATKAMHWYDGEGNLFYRH
                     GDIGRIDEDGFLTLMDRAKDMIISGGFNIFPSDLEAILIADDRVVEAAVVGMPSEEWG
                     ETPVAFVVLTEGAEAESVRADCNAKVGKTQRMSAIRVVDELPRSPIGKVLKRELRDAH
                     AG"
     gene            2086..2754
                     /locus_tag="VN034_12465"
     CDS             2086..2754
                     /locus_tag="VN034_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV61467.1"
                     /translation="MAVYHPVAERADALAAAILATGLTVQRLPQHVDGLRAWVNSCFD
                     LLLVNPFLDWQEPFEFVRLARSIAGRRPLLVVSDRDALGDRMKALGAGADDAVGWTDN
                     LPEVLARIASLLRRSRIAAGQLGEGELRIDLIDRRVERAGRLIRLPLREFDLLANLAR
                     VPDRPLSRDALLRAVWRIDFDPGTNRVEVHMSRLRAKVDRGFDWPMLHTVKGRGYALR
                     SRPD"
     gene            2852..4384
                     /locus_tag="VN034_12470"
     CDS             2852..4384
                     /locus_tag="VN034_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWV61468.1"
                     /translation="MHPSVHARANPDKAAIIVAETGEAISYGELDAASNRAAQLFRSH
                     GLGHEDVVAFMLDNTPHYYGLTWGAQRAGLRYVCISSRLTQDETDYILENSGAKILIV
                     SASLADAAQQLTTGIKRFAMGGDIPGYESWEDAVAAMPATPVADERAGVDMLYSSGTT
                     GRPKGVRVPLPEDPAIDAVNSLVMLASAVFQINADSIYLSPAPLYHAAPLRWSMTIHR
                     LGGTVVLMKKFDPEAALSHIEKYRVNSSQWVPTHFVRMLKLPGDVRTRYDTSSLKVAI
                     HAAAPCPVPVKQAMIDWWGPVLFEYYAGSEGNGMTFISSADWLTHRGSVGRPILGTVH
                     IMGEDNETELGPDEEGAVFFESENIFEYHGDNEKTASSRNSKGWSTLGDVGKVDEEGF
                     LYLTDRKSFMIISGGVNIYPQEIENHLVTHPKVADVAVVGGPHDEMGEEVIAVIQPAN
                     MADATEELRDELIAYAREKLSGVKIPRRIDFLEALPRHDTGKLYKRLLRDQYWEKAKA
                     EA"
     gene            4387..>4833
                     /locus_tag="VN034_12475"
     CDS             4387..>4833
                     /locus_tag="VN034_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWV61469.1"
                     /translation="MTARVEFFFDLSSPWTCLAFHNLPGVLERAGAAAIYRPILVGGV
                     FNAVNPAVYAAREQVDNRRLTHSWKVLKDWARLAGVPMNFPSQWHPAKSIAAMRFCCA
                     LEEDQAALVLFARGAFASYFDRQENLDDPAVLTAVADAEGLDGAALA"
BASE COUNT          833 a         1557 c         1532 g          911 t
ORIGIN      
        1 gcgccacaaa tcgggttcga acatcctgag attgactccc atgcgctggt aattgggatc
       61 agttggcgtc cagtgtgtcg tacagccaca ggcttggcag tgccacgtgt cgagtgcgcg
      121 atcaccctgc tggtaggcta ccccctctcc ctcgaccgtg accgatttcg gcgggcaata
      181 gtgccacagt ccggcggtac gccgacaaat cgagcaattg cattcgctga cttcggttgg
      241 ctcgtcgcgc actaccaagt gaacccgccc gcaatggcag ctgccgtggt gctcgatcat
      301 cgtcgctcct tccaactcaa gtcatcttga gcgcctactt ttaacaatcc cgccagttca
      361 aagcgacgac cgaaaacggt cgttttcagc ccttgggctt ctctgcctct tactcacaat
      421 ttacccagcg tgggcatcgc gcaattcgcg cttcagcacc ttgccgatcg ggctgcgcgg
      481 cagctcgtca accaccctga tcgcgctcat ccgctgcgtc ttgccgacct tggcattgca
      541 gtccgcccgc acgctttcgg cctccgcgcc ttccgtcagc acgacgaacg ccaccggcgt
      601 ctctccccat tcctcactcg gcatccccac gaccgccgcc tcgacgacgc gatcgtcggc
      661 gatcaatatc gcctcgaggt cgctcgggaa gatgttgaag ccgcccgaaa tgatcatgtc
      721 ctttgcgcgg tccatcagcg tcaggaaacc gtcctcgtcg atccgtccga tgtcgccgtg
      781 gcgatagaag agatttcctt cgccatcata ccagtgcatc gccttggtcg cgtcggggcg
      841 gttgttgtat ccggtcatca tcgcggggct gcggccgacg acttcgccga ccgatccttg
      901 cggcaattcc ttgccctcct cgtcgatcac cttcgcgata tgcccgggcg cgggcttgcc
      961 gacggtgtgc agcttgtcgg taaattggtg cgcctcgagg atgaacgccg cgccgccttc
     1021 ggtcataccg taaatctcga ccagcccgcc cggccagcgc tccagcacat cggcttttag
     1081 cgccgcgggg aagggcgccg aggtgcaata tttcatcacg aaacttgaaa ggtcgaagct
     1141 ggcgaaatcg tcgagcgcca tgatgcggcg atattggacc ggcaccagca tgcagttcgt
     1201 ggcgcgttcc cgttcggcaa gttcgaggaa accgcgcgcg tcgaatttct tcatcagcac
     1261 gacctgcccg cccgacccga ccgtaggcat gaagctcgcc atcgttgtgt tcgaatagag
     1321 cggggtcgac aggatcgtca cggcgttcgg gccataagcg ggcgcaccac ggatgatatg
     1381 ctgccagcgc atcgcatggc tgtggacgat gcctttgggc gtgcccgtcg ttccagacga
     1441 atagatgatg ttgaacccgt cctcgggccg gatgtcgacc ggggcgggtt tgacgcccgc
     1501 gagggacagc caggcgtcca gcggcgctcc cgcgtcgctg ccgtccatcg cgatcaggtc
     1561 gctcgcggcg atgacctgcc cgtcgaggct cgatttcgct gcgctgtcga ggaacaggtg
     1621 gcgcgcgccg gtgtcggcga tcatcgccgc cgtctgctcg cccgtcgcgc tgttggtgac
     1681 gagccccgcg atgccgccgg cgcgcaaggt gccgagaatc accgccatct gctcgacgct
     1741 gttgaggccg gcgatcgcgg tgcggtcgcc ctttgtaaat ccgtcctgtt gcagccgggc
     1801 ggcgattcgg tcggtcaatt gatccagttc ggaccagctc agccgccgga acgggtcggc
     1861 agccgctacg gcatcgggac gctccgcggc atgggcacgg acaaggtcgg gcagggtggc
     1921 gaagtcgcct tcaagcattt cgatactggt catgcctatc gactagcaca gccgaaaggc
     1981 ggtgcaatgc cggtggggcg gcgaggcgac aagatgttgc gtcgacgacg ggggatgtgg
     2041 atggcctcat cgaatattgt cggggtgggc gacaggaaat tactggtggc cgtctatcat
     2101 cccgtcgcgg agcgggcaga tgcgctggcg gcagcgatcc tcgccacggg tctcacggtg
     2161 cagcggctgc cgcaacatgt cgacgggctc cgcgcatggg tcaatagctg tttcgacctg
     2221 cttctcgtca atccgtttct cgactggcag gaaccgttcg aattcgtgcg gctggcgcga
     2281 tcgatcgcag ggcggcggcc gctgctggtc gtatcggacc gcgacgcgct cggcgaccgg
     2341 atgaaggcgc tgggcgcggg cgcggacgat gccgtcggct ggaccgacaa tctccccgaa
     2401 gtgctcgcgc ggatcgcgag cctgctccgc cgcagccgca tcgccgcggg ccagctcggc
     2461 gagggcgaac ttcgtatcga cctgatcgat cgccgcgtcg agcgcgcggg gcggctcatc
     2521 cgcctgccgc tgcgcgagtt cgacctgctc gccaatctcg cacgcgtccc cgaccgtccg
     2581 ctgtcgcgcg atgcgttgct ccgtgcggtg tggcggatcg atttcgatcc cggcaccaac
     2641 cgggtcgagg tgcatatgtc gcggctgcgc gccaaggtcg atcgcggctt cgactggccg
     2701 atgctccata cggtgaaagg gcggggctat gcgctgcgct cgcggcccga ttgacgatcc
     2761 tgtcacgagc cccagcttgc ttgaccaaaa gtttcaggtt gctactcatc ttcgacataa
     2821 agaaaccagc gattcccgga gatgatgccc gatgcacccc tcagtccacg cccgcgccaa
     2881 ccccgacaaa gccgcgatca tcgttgcgga gacgggcgag gcgatcagct atggcgaact
     2941 cgacgcagcc tcgaaccgcg ccgcgcagct ctttcggtcg catgggctgg ggcacgagga
     3001 tgtcgtcgcc ttcatgctcg acaacacgcc gcattattac ggcctcacct ggggcgcgca
     3061 gcgtgcgggg ctgcgctatg tgtgcatctc gtcgcggctg acgcaggatg agaccgacta
     3121 tatcctcgaa aattcgggcg cgaagatcct gatcgtgtcg gcgagcctcg cggacgcggc
     3181 gcagcagctc acgaccggga tcaagcgttt cgcgatgggc ggcgacattc cgggctatga
     3241 aagctgggag gatgccgttg cggccatgcc cgcgaccccc gtcgccgacg agcgcgccgg
     3301 ggtcgacatg ctctattcgt cgggcaccac cggccgcccc aagggcgtgc gcgtgccgtt
     3361 gcccgaggat ccggcgatcg acgcggtcaa cagcctcgtc atgctcgcct cggcggtgtt
     3421 ccagatcaat gcggacagca tctatctctc gccggcaccg ctctatcacg ccgcgccttt
     3481 gcgctggtcg atgacgatcc accgcctcgg cggcaccgtc gtcctgatga agaaattcga
     3541 tcccgaggcg gcgctgtcgc acatcgaaaa atatcgcgtg aattcgagcc agtgggtgcc
     3601 gacgcatttc gtgcggatgc tcaagctccc gggcgacgtc cgaacccgct acgacacctc
     3661 ctccttgaag gtcgcgatcc atgccgcggc gccgtgcccg gtcccggtca agcaggcgat
     3721 gatcgactgg tggggcccgg tgctcttcga atattatgcg gggtccgaag gcaatggcat
     3781 gaccttcatc tcgagcgccg actggttgac gcaccggggc agcgtcggcc gcccgatcct
     3841 cggcaccgtc catatcatgg gcgaggataa tgagaccgaa ctcggccccg acgaggaagg
     3901 cgcggttttc ttcgaaagcg aaaatatctt cgaatatcat ggggataatg agaagacggc
     3961 ttcgagccgt aattcgaagg gctggtcaac tttgggcgat gtgggcaaag ttgatgaaga
     4021 aggcttcctc tatctcaccg accgcaagag cttcatgatt atctcgggcg gcgtgaacat
     4081 ctatccgcag gaaatcgaga atcatctcgt cacccacccc aaggtcgccg acgtcgcggt
     4141 cgtcggcggc ccgcacgacg aaatgggcga ggaagtgatc gcggtgatcc agcccgcgaa
     4201 tatggccgac gcgaccgagg aattgcgcga cgaactgatc gcctatgcgc gcgagaaatt
     4261 gtcgggcgtg aaaatcccgc gccggatcga cttcctcgaa gccctgccgc gtcacgacac
     4321 gggcaagctc tacaagcgcc tgctgcgcga tcaatattgg gaaaaggcca aggcggaggc
     4381 ctgagcatga ccgcgcgcgt cgaattcttc ttcgacctct cgtcgccctg gacctgcctt
     4441 gcgtttcaca atctgccggg cgtgctcgaa cgcgccggcg ctgcggcgat ttaccggccg
     4501 attctcgtcg gcggggtgtt caacgcggtg aaccccgccg tctatgcggc gcgcgaacag
     4561 gtcgacaacc ggcggctgac gcacagctgg aaggtgctga aggactgggc gcggcttgcg
     4621 ggtgtgccga tgaatttccc gtcgcaatgg catccggcaa agagcatcgc cgcgatgcgc
     4681 ttctgctgcg cgctcgaaga ggatcaggcc gcgctcgtgc ttttcgcgcg cggtgcgttc
     4741 gcgagctatt tcgaccggca ggagaatctc gacgatccag ccgtgctgac ggcggttgcc
     4801 gatgccgagg ggctcgacgg cgcggcgctc gcc
//
LOCUS       DATMSU010000468         1804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_112053,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000468 DATMSU010000000
VERSION     DATMSU010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112053"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..>1804
                     /locus_tag="VN034_12480"
     CDS             335..>1804
                     /locus_tag="VN034_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWV61470.1"
                     /translation="MKTSYLDYAMSVIVSRALPDVRDGLKPVHRRILYAAQEGGFIPG
                     RPYKKSAKIVGDVMGNYHPHGDSAIYDALARMTQDWSLRVPLIDGQGNFGSMDPDPPA
                     SMRYTEARLAKTAMVLLNDLDKDTVDFQPNYDASREEPTVLPARFPNLLVNGAGGIAV
                     GMATNIPPHNLGEIIDATLAMIDRQLEGGEPVTLEELMAIVPGPDFPTGAMMLGQGGA
                     RNAYATGRGSIMMRATHIIEEGRNDRQSIVLTSIPFQVGKSGLVEKIAEAARDKRIEG
                     VADIRDESNREGVRVVIELKRDATAEVVLNQLWRHTPAQSSFPANMLAIRGGRPEMLG
                     LKDILSAFITFREEVITRRCKFELAKARDRAHILLGLVVAVSNLDEVVRIIRGSNSPA
                     AAREALLAREWPIGEIAPYIRLVEAIEGEMEEAATYRLSEVQVKAILDLRLHRLTALG
                     RDEIGKELGELATEIEELLSILADRVKLYGVMREELVAVR"
BASE COUNT          356 a          614 c          546 g          288 t
ORIGIN      
        1 gctggcgccg accagcgcaa tcagggccat cggcgccggg ggtactgaac agcggtcatc
       61 ggcgcgcagc tttagggacg gtgaagcgat tggcaagccc cgcgatgggg cccgatgacg
      121 tcacgccttc atcccgtttt cccgcacttt acgcgccatt tctgtgcgca aaaaaatagc
      181 ggaaaaatga ccgaatcgct ggcctttcca cccaccatcg gctaggaaaa aatcatctcc
      241 cgaaaagcag aaaaaaggcc gggtcttgac cgaagaaaat acgcctacgc cgccgtccga
      301 ggatggcgtt tcgccgatca acatcgtcga cgaaatgaag acgtcatacc tcgattacgc
      361 gatgagcgtg atcgtcagcc gcgcgctccc cgacgtgcgc gacgggctga agccggtgca
      421 tcgccgcatc ctttacgcgg cgcaggaagg cggcttcatc ccgggccgcc cgtacaagaa
      481 atcggcgaag atcgtcggcg acgtgatggg taactatcac ccgcacggcg acagcgcgat
      541 ctacgacgcg ctcgcgcgca tgacgcagga ctggtcgctt cgcgtgccgc tgatcgacgg
      601 tcagggcaat ttcggctcga tggaccccga tccgccagca tcgatgcgtt acaccgaagc
      661 gcgcctcgcc aagaccgcga tggtactgct gaacgatctc gacaaggata cggtcgactt
      721 ccagcccaac tatgacgcga gccgcgaaga gcccaccgtg ctcccggcgc gcttcccgaa
      781 cctgctcgtc aacggcgccg gcggcatcgc ggtcggcatg gcgaccaata tccccccgca
      841 taacctcggc gaaatcatcg acgccacgct cgcgatgatc gaccgccagc tcgaaggcgg
      901 cgaacccgtc acgctcgagg aattgatggc gatcgtcccc ggtcccgatt tcccgaccgg
      961 cgcgatgatg ctcggccagg gcggcgcgcg caacgcctat gccacgggcc gtggttcgat
     1021 catgatgcgc gcgacgcata tcatcgagga gggccggaac gaccgccaat cgattgttct
     1081 gacgtcgatt cctttccagg tcggcaagtc cggcctcgtc gagaagatcg ccgaagcggc
     1141 gcgcgacaag cgcatcgagg gcgtcgccga catccgcgac gaatcgaacc gcgagggcgt
     1201 gcgcgtcgtc atcgagctga agcgcgacgc gaccgccgag gtcgtcctca accagctctg
     1261 gcgccacacc ccggcgcagt cgagcttccc cgccaacatg ctcgcgatcc gcggcggccg
     1321 ccccgaaatg ctgggcctga aggacattct ttcggccttc atcaccttcc gcgaggaagt
     1381 gatcacccgc cgctgcaagt tcgaactcgc caaggcgcgc gaccgcgcgc acatcttgct
     1441 tggcctcgtc gtcgcggtca gcaatctcga cgaagtcgtc cgcatcatcc gcggctcgaa
     1501 ctcgcccgcc gccgcccgcg aagcgctgct cgcgcgcgaa tggccgatcg gcgagatcgc
     1561 gccctatatc cgcctcgtcg aggcgatcga gggcgagatg gaggaagccg ccacctaccg
     1621 cctgtccgaa gtgcaggtga aagcgatcct cgacctccgc ctccaccgcc tcaccgcgct
     1681 tggccgcgat gaaatcggta aggaactcgg ggagttggcg accgagattg aggaactgct
     1741 ttcgatcctc gccgatcgcg tcaaactcta tggcgtgatg cgcgaggaac tcgtcgcggt
     1801 gcgc
//
LOCUS       DATMSU010000469         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_159674,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000469 DATMSU010000000
VERSION     DATMSU010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159674"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..560)
                     /locus_tag="VN034_12485"
     CDS             complement(117..560)
                     /locus_tag="VN034_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HWV61471.1"
                     /translation="MTVTKAQPFWEAPLASLDAGQWEALCDGCGKCCLHKLEDEDTGR
                     IYPTNVACRLLDLTTARCGDYKHRRRHVPDCLTLTRAKVDDLEWLPQSCAYRLRAEGE
                     PLPDWHYLVCGDRDAVHRAGQSIVGWTVGEDVAGPLENHLVERVI"
     gene            complement(557..1201)
                     /locus_tag="VN034_12490"
     CDS             complement(557..1201)
                     /locus_tag="VN034_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWV61472.1"
                     /translation="MMNIANMGQRLVRASLILAFGAALAGCNGSGESAPAKPPLEGAR
                     IGGTFTLTDQNGKTVRDSDFAGQYRLVYFGYSFCPDICPVDLQKLMRGLSQFEKADAA
                     RGAKVAPLFITVDPERDTPEALKPFVARYHPRLLGLTGTPEQIASVAKAFVVTYNKVP
                     GSAPDRYLMAHSQLAFLMDPDGKPLALLPLDDPSTDVDEGAPGKVAAELAKWVK"
     gene            1290..1907
                     /locus_tag="VN034_12495"
     CDS             1290..1907
                     /locus_tag="VN034_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWV61473.1"
                     /translation="MFRRAEFRFASLLLMLLAVAGLVHPAQAQFRGGYAFLQAVENRD
                     GTKATEALKDDPSFVNTRNTDTGETALIIVTKRRDISWLRFLLAKGADPSIGDRQEVT
                     PLMHAALINFTDGAQELLDSKAPVDQNNRRGETALILAVQAKNVAMVRLLVKHGANPD
                     KADYIAGMSARDYAKRDGRSGRILELLEAKADAPAREFGPTFGPQ"
     gene            1965..3239
                     /gene="ribB"
                     /locus_tag="VN034_12500"
     CDS             1965..3239
                     /gene="ribB"
                     /locus_tag="VN034_12500"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWV61474.1"
                     /translation="MSTMLIDRIRAIVDDGTMSRSGLARAAGLHANSLRDLDSPGWNP
                     TAETLRKLENWLANGSDLSPMASPEEIIAEARNGRMFILVDDEDRENEGDLVIPAQMA
                     TPDAINFMVTHGRGLVCLTLTKARVDTLGLELMSRNNGTRHETAFTTSIEAREGVTTG
                     ISAGDRARTVAVAIDAGKTRDDIVTPGHIFPLIARDGGVLVRAGHTEASVDIARLAGL
                     NPSGVICEIMNDDGTMARLDDLIPFARRHGLKIGTIADLIAYRNRSDRLVECVSDDPF
                     ESDYGGDWRLKSYRNKIDGSVNLVLQKGPVDPEGVTLVRMHPVSLFDDIMGRPGPKKR
                     RLQRSMDAIGEAGAGVIVMLMRPLPGSADADALPPPTGGMDLRTYGIGAQILADLGVH
                     AMELLTPTHSNIVGLEGYGLSVVGERPIPGEA"
     gene            3247..4098
                     /locus_tag="VN034_12505"
     CDS             3247..4098
                     /locus_tag="VN034_12505"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWV61475.1"
                     /translation="MNEGLGIGFAVVAALTTASSHALLKSGEDQEAVRALCGVTWAAA
                     AAIPLTFIGAPAPAILPWLAGAVTLHSLYSLVLTRSYTLNDFSVAFPIARGTAPLVAT
                     AAGAPLFAETPGVGEILGVAAICAGIFSLSAGGRIGTKGLIAALGAGVLTAAYTVVDA
                     GGMRASDGVLPFFCWFFFATGIALIAQFRASAGPSAFARMRNNLRPGIAAGSLAIVSF
                     GSTLVALRYAPVAVVSALRETCILVSLLIAWFWMKERITLHSLTAAILIAAGALLLLL
                     PHLFPSI"
     gene            4109..4534
                     /gene="ribH"
                     /locus_tag="VN034_12510"
     CDS             4109..4534
                     /gene="ribH"
                     /locus_tag="VN034_12510"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWV61476.1"
                     /translation="MAHVLIVEARFYSHLNDMLLDGVRSALEGEGHSHETVTVPGALE
                     VPAAISLAADSGRYDAYVALGVVIRGETYHFEVVSNESARGIMALTLDGLAIGNGILT
                     VENEEQALARADKTRKDKGGEAAKAALAMLALKEQFGIG"
     gene            4527..>4749
                     /locus_tag="VN034_12515"
     CDS             4527..>4749
                     /locus_tag="VN034_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61477.1"
                     /translation="MVDRPPPSRFSVVERGGRLVVIDRETGRTPPTAAERMDAHDRRM
                     DVEANRPAARTAPVDAETVLDLAQAAPAPR"
BASE COUNT          792 a         1599 c         1554 g          804 t
ORIGIN      
        1 gctcgccgcg cgcgccgtcg aagacgaggc ggtagcggcg cgactgcgca tcgcgcacca
       61 cgcgcaccgg ccaagcttcg tcgccgaccc ggatgtgcgg ccgatcggcc gacggatcaa
      121 atgacgcgtt cgacaagatg attctcaagc gggcccgcga catcctcgcc gaccgtccag
      181 ccgacgatcg actggcccgc gcggtgcacc gcatcgcggt cgccgcaaac gagataatgc
      241 cagtcgggca ggggctcgcc ctcggcgcgc aggcgatagg cgcagctctg cggcagccat
      301 tcgaggtcgt cgaccttcgc tctggtcagc gtcaggcagt cgggaacgtg gcggcggcga
      361 tgcttgtagt cgccgcagcg cgcggtcgta aggtcgagca ggcggcacgc gacattggtc
      421 ggatagatgc ggcccgtatc ctcgtcttcc agcttgtgca ggcagcattt tccgcagccg
      481 tcgcacaagg cctcccattg cccggcgtcg aggctggcga gcggcgcttc ccagaagggc
      541 tgcgccttcg tcacggtcat ttcacccact tcgccagctc ggcggcgacc ttgccgggcg
      601 cgccctcgtc gacatcggtc gacgggtcgt cgagcggcag cagcgcgagc ggcttgccgt
      661 cgggatccat cagaaaggcg agctggctgt gcgccatcag atagcgatcg ggcgccgagc
      721 ccggcacctt gttataggtg acgacaaagg ctttcgcgac gctcgcgatc tgttcggggg
      781 tgccggtcag gccgagcagg cgcgggtgat agcgcgcgac gaagggcttc agggcctcgg
      841 gcgtgtcgcg ctcgggatcg acggtgatga agagcggcgc gaccttggcg ccgcgcgcgg
      901 cgtcggcctt ttcgaactgc gacaggccgc gcatcagctt ttgcaggtcg accgggcaga
      961 tgtcgggaca gaagctgtaa ccgaaataga cgaggcgata ttggccggcg aaatcgctgt
     1021 cgcggacggt cttgccattc tggtcggtca gcgtgaaggt gccgccgatg cgcgcgccct
     1081 cgagcggcgg cttggcgggc gcgctttcgc ccgagccgtt gcatcccgcg agcgccgcgc
     1141 cgaaagcgag gatcaggctt gcacggacaa gcctttgtcc catatttgcg atattcatca
     1201 tgtggccagc cttggtcggc tagcttgcga aaatcaacca ttgtcgtcgc ggcgcgcgat
     1261 ggacaaattg ccccaggggt cgcccgatca tgtttcgacg cgcggaattc cgcttcgcct
     1321 ccttgctcct catgctgctc gcggttgcgg gtctcgttca ccccgcgcag gcgcagttcc
     1381 gcggcggcta cgcctttctg caggcggttg aaaaccgcga cgggaccaag gcgaccgagg
     1441 cactgaagga cgatccgtcc ttcgtcaaca cgcgcaacac cgacaccggc gaaaccgcgc
     1501 tgatcatcgt caccaagcgg cgcgatatca gctggctgcg cttcctcctc gccaagggcg
     1561 ccgatccgtc gatcggcgat cgtcaggagg taacccccct gatgcatgcc gcgctcatca
     1621 acttcaccga cggggcccaa gagctgctcg acagcaaggc acccgtcgac cagaacaacc
     1681 ggcgcggcga aaccgcgctg atcctcgcgg tgcaggcgaa aaatgtcgcg atggtccgcc
     1741 tgctcgtcaa gcacggcgcc aatcccgaca aggccgacta tatcgcgggt atgtcggcgc
     1801 gcgactacgc caagcgcgac ggtcgcagcg gacgcattct cgagcttctc gaagccaagg
     1861 ccgatgcacc ggcgcgcgag ttcggcccga ccttcggccc gcaatgatca aggcaatgtg
     1921 atagtccgat tgacaatcac attatgatgt gagacatgac tatcatgtcc acaatgctca
     1981 tcgatcgtat ccgcgccatc gtcgacgacg ggaccatgtc ccgctcgggt ctcgctcgcg
     2041 ccgcgggtct ccacgccaac agcctgcgcg acctcgattc gccgggctgg aatcccaccg
     2101 cagaaacgct gcgcaagctc gaaaactggc tcgcgaacgg cagcgacctg tcgccgatgg
     2161 cgagcccgga ggagatcatc gccgaggcgc gcaacgggcg catgttcatc ctcgtcgacg
     2221 acgaggaccg cgagaatgaa ggcgacctcg tcatacccgc gcagatggcg acccccgacg
     2281 cgatcaattt catggtcacc cacggccgcg gcctcgtctg cctgacgctg accaaggcgc
     2341 gcgtcgatac gctggggctc gaactgatga gccgcaacaa cggcacgcgg cacgaaaccg
     2401 ccttcaccac ctcgatcgaa gcgcgcgaag gcgtgacgac gggcatttcg gcgggcgacc
     2461 gcgcgcgcac cgttgccgtg gccatcgacg ccggcaagac ccgcgacgac atcgtgacgc
     2521 cgggccatat cttcccgctg atcgcgcgcg atggcggcgt gctcgtgcgc gccggccata
     2581 ccgaagcctc ggtcgacatc gcgcgcctcg cggggctcaa cccctcgggc gtgatctgcg
     2641 agatcatgaa cgacgacggc acgatggcgc ggctcgacga cctcatcccc tttgcgcggc
     2701 gccacgggct caagatcggc acgatcgccg acctcatcgc ttatcgcaac cgcagcgacc
     2761 ggctggtcga gtgcgtgtcc gacgatccgt tcgaatcgga ctatggcggc gactggcggc
     2821 tcaaatccta tcgcaacaag atcgacggca gcgtcaatct ggtgctgcaa aaggggccgg
     2881 tcgatcccga aggcgtcacg ctcgttcgca tgcacccggt gtcgctgttc gacgacatca
     2941 tgggccgccc gggccccaag aagcgccgcc tccagcgatc gatggacgcg atcggcgaag
     3001 ccggtgcggg ggtgatcgtg atgctgatgc gcccgctccc cggctcggcc gatgccgacg
     3061 cactcccgcc accgaccggc ggcatggatc tgcgcaccta tggcatcggc gcgcagatcc
     3121 tcgccgacct cggcgtccat gcgatggaat tgctcacccc cacccacagc aatatcgtcg
     3181 gcctcgaagg ctatggcctg tcggtcgtcg gcgaacgccc catccccggc gaggcgtgat
     3241 cggacggtga acgagggact ggggatcggc tttgcggtcg tagccgcgct gaccaccgct
     3301 tcgtcgcatg cgctgctcaa gtcgggagag gatcaggagg ccgttcgtgc gctgtgcggg
     3361 gtcacctggg ctgcggcggc cgccattccc ctgacgttca tcggcgcgcc ggccccggcg
     3421 atactgccgt ggctggccgg cgccgtgacc ttgcacagcc tctattcgct ggtgctgacg
     3481 cggtcgtaca cgctcaacga tttttcggtc gcctttccga tcgcgcgggg aaccgcgccg
     3541 ctcgtcgcga cagccgccgg tgcgccgctg tttgccgaaa cgcccggtgt cggagagata
     3601 ttgggggtag ccgcgatctg cgccggcata ttctcgctgt ctgcgggcgg gcgcatcggc
     3661 acgaaaggat tgatcgccgc gctgggcgcg ggggttctga ccgccgctta taccgtcgtc
     3721 gatgcgggcg gaatgcgggc gagcgacggg gtccttccgt ttttctgctg gttcttcttc
     3781 gccaccggga tcgcactgat tgcccagttc cgggcgtcgg ccggcccttc cgctttcgcg
     3841 cgcatgcgaa acaatctgcg gcccggaatc gcggccggat cgctcgcgat cgtcagcttc
     3901 ggatcgacac ttgtcgcgct gcgttacgcg cccgtcgccg tcgtcagcgc gctgcgcgaa
     3961 acatgtatcc tcgtcagcct gttgatcgca tggttctgga tgaaggagcg catcacgctt
     4021 cacagcctga ccgccgctat cctgatcgcg gcgggcgccc tgcttctcct gcttccccat
     4081 ctcttcccat ccatctaagg aggccgtcat ggcacatgtt ctgatcgtcg aagcccgctt
     4141 ctattcgcac ctcaatgata tgctgctcga tggcgtgcgc agcgcgctcg agggcgaggg
     4201 gcatagccac gagacggtga cggtgcccgg cgcgctcgaa gtgcccgcgg cgatttcgct
     4261 cgccgccgac agcgggcgtt acgacgccta tgtcgcattg ggcgtcgtga tccgcggcga
     4321 gacctatcat ttcgaggtcg tctcgaacga aagcgcgcgt ggcatcatgg cgctgacgct
     4381 cgacggcctc gcgatcggca acggcatttt gaccgtcgag aatgaggaac aggcgcttgc
     4441 gcgcgccgac aagacgcgca aggataaggg cggcgaagcg gccaaggccg cgctcgccat
     4501 gctcgcgctc aaggaacaat tcggtattgg ttgaccgtcc gcctcccagc cgtttttcgg
     4561 tcgtcgagcg cggcgggcgg ctagtggtga tcgaccgcga aaccggccgg actccgccga
     4621 ccgcggccga gcggatggat gcccatgatc gccggatgga tgtggaggcg aaccggcccg
     4681 cagcgcggac tgcaccggtc gatgccgaaa cggtcctcga ccttgcgcaa gccgcaccgg
     4741 cgccccggc
//
LOCUS       DATMSU010000470         3887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_159687,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000470 DATMSU010000000
VERSION     DATMSU010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159687"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..3197)
                     /locus_tag="VN034_12520"
     CDS             complement(3..3197)
                     /locus_tag="VN034_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV61478.1"
                     /translation="MSRLFINRPIFAWVLAIIVMLGGVGALFSLPIEQYPDIAPTQVN
                     IRASYPGASAETIENSVTQVLEQQLTGIDGLLYFSSQSSSRGRADITAIFVKGTDPDI
                     AQVQVQNKIQSAVSRLPQQVQAQGVRVTKSNSDSLLLVGVYDTTDTRSNQDVSDYLSS
                     NIQDPLSRVEGVGDVNVFGSPHAMRIWLNPQRLAAMSLMPSDVVSAISAQNSEVAAGE
                     VGGLPAPEGQMLNATVTAQSRMQTAEQFENIVLKTLPDGATVRIKDVARVEIGAENYS
                     SIVRINGHPGSGMSISLSPGSDALETADRVKARMTELAADFPDGLTYSYSNDSTAFIK
                     LSVSEVQKSLFEAILLVILVMFVFLQSWRAVLIPAIAVPVVLLGTFGIFYLLGFSINT
                     LTLFGLTLAIGLLVDDAIVVVENVERLMEENPGMSAREATIQSMQELQVALIAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSVTIVSAMALSVLVALILSPALTSTLLKPKNGDGGTD
                     SRFPRVHAFLERAKNGFNTRFDGAVNRYVGSVTKVVDRKWLFLGIYAVLLALLAFLFL
                     RLPGGFLPNEDQGRVSIQFRLPAGATQARTLEVRDTVEKYLLTEEKANVGALFLIAGG
                     GGGGASGQNTGQGYVNLVHWDERPGEERSADAIVERARAALAGVRDAQIFALVPGAVR
                     GLGDSSGFTMQFQNRSGMSRDEFVAARDRLLAMANDNPRLTSVRLSDLPDVSTLKIDV
                     DTQRLTAYGIDNGDVNNTLATAWGGRYVNDFIDKGRVKRVYVQGDAPYRASPEDLGQW
                     FVRSVGGQMSPFSAFATIGWSTTPSSSSRFQGVPAFEISGQPSPGTSSGEAMDEMERM
                     ANQIPGTSVAWSGSSYEERLSSGQAPLLYGLSLLVVFLCLAALYESWSIPLAVLLVIP
                     LGLIGAVFAVNLRGLENDVYLQIGLLTTMGLAAKNAILMIEFAEQEERKGKRVIEAAV
                     EAARIRLRPILMTSFAFIFGVLPLAIATGAGANSRVAIGTSVIGGMLTAAFLAIFFIP
                     LFFVLVRRGVRDGLAAMRARFGKKKGDGAAEVPA"
     gene            complement(3204..>3887)
                     /locus_tag="VN034_12525"
     CDS             complement(3204..>3887)
                     /locus_tag="VN034_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV61479.1"
                     /translation="INLRFATITAPISGRIGRSLVTPGGLVSASQATPLAVIQQTDPM
                     FVDMQQSSAELTALRQAIESGGVNAGSTSVRLRLEDGSNYGFAGTVQFSDVTVNEATG
                     TVTLRARFPNPKGVLLPGMFVTAMFDQAVNPSAILLPQPAVQRDFDGSAFVYLVGKDN
                     KAVRRKIVADRTSGANWVVTDGLKPGERVISQGIGNLRQGAAVRPVPASSAQRVGAPK
                     GEGAGAKGK"
BASE COUNT          690 a         1286 c         1289 g          622 t
ORIGIN      
        1 ggtcatgccg gcacctcggc ggcgccatcg cccttcttct tgccgaagcg cgcgcgcatc
       61 gcggcgagcc cgtcgcgcac gccgcggcgg acgaggacga agaagagcgg gatgaagaag
      121 atcgcaagga aggcggcggt gagcatgccg ccgatcaccg aggtgccgat cgcgacgcgg
      181 ctgttcgcgc ccgcaccggt cgcgatcgcg agcggcagca cgccgaagat gaaggcgaag
      241 ctggtcatca ggatcggacg caggcggatg cgcgccgcct cgaccgccgc ctcgatcacc
      301 cgcttgccct tgcgttcctc ctgctcggcg aattcgatca tcaggatcgc attcttcgcc
      361 gccagcccca tcgtcgtgag caggccgatc tggagataga cgtcgttctc gagcccgcgc
      421 agattcaccg cgaacaccgc cccgatcagc cccagcggaa tgacgagcag caccgcgagc
      481 gggatcgacc agctttcgta aagcgccgcg aggcagagga agacgacgag caaagacagg
      541 ccgtaaagca agggcgcctg ccccgaggag aggcgctctt cataggagga gcccgaccag
      601 gcgacgctgg tgcccgggat ctggttcgcc atgcgctcca tctcgtccat cgcctcgccc
      661 gagctggtgc cgggggatgg ctggcccgag atttcgaagg cgggaacgcc ctgaaagcgc
      721 gagctgctgc tcggcgtcgt cgaccagccg atcgtggcaa aggccgagaa gggcgacatc
      781 tgtccgccca ccgagcgaac gaaccattgg ccgagatcct cgggactggc gcgatagggc
      841 gcgtcgccct ggacatagac gcgcttgacg cggcccttgt cgatgaaatc attgacgtag
      901 cggccgcccc acgcggtcgc gagcgtgttg ttcacatcgc cattgtcgat gccataggcg
      961 gtcagccgct gcgtatcgac gtcgatcttg agcgtcgaga cgtcgggcag gtcggacagg
     1021 cgcacagacg tcagcctggg gttgtcgttc gccatcgcga gcaacctgtc gcgcgcggcg
     1081 acgaattcgt cgcggctcat cccgctgcgg ttctggaact gcatcgtgaa gcccgacgaa
     1141 tcgccgagac cgcgcaccgc gccggggacg agcgcgaata tctgcgcgtc gcgcaccccg
     1201 gcgagcgcgg cacgcgcgcg ttcgacgatc gcgtcggcgc tccgctcctc gcccggccgc
     1261 tcgtcccaat ggacgaggtt gacgtagccc tggccggtat tctgccccga cgcgccgcca
     1321 ccgccgccgc cggcgatgag gaacaacgcg ccgacattcg ccttttcctc ggtcagcaga
     1381 tatttttcga ccgtgtcgcg cacctcgagc gtgcgcgcct gcgttgcgcc cgccggcagg
     1441 cggaactgga tcgagacgcg cccctgatcc tcgttgggca ggaaaccgcc cggcaggcgg
     1501 agaaagagga aggcgaggag cgcgagcagc accgcataga tgccaaggaa cagccatttg
     1561 cggtcgacga ccttggtaac gctgccgaca tagcggttga ccgcgccgtc gaagcgcgtg
     1621 ttgaaaccgt tcttggcgcg ttcgagaaac gcgtggacgc gcgggaagcg gctatctgtg
     1681 ccgccgtcgc cattcttggg cttgagcagg gtcgaggtca gcgcggggct caggatcagc
     1741 gcgacgagca ccgacaaggc catcgccgac acgatggtga ccgagaactg gcgatagatg
     1801 acgccggtcg acccgccgaa aaaggccatc ggcaggaaca ccgccgacag caccagcgcg
     1861 atcgcgatca gcgcgacctg cagttcctgc atcgactgga tcgtcgcctc gcgcgccgac
     1921 atgccgggat tttcttccat caaccgctcg acattctcga ccacgacgat cgcgtcgtcg
     1981 acgagcaggc cgatcgcgag cgtcagcccg aacagcgtca gcgtgttgat gctgaacccc
     2041 agcagataga agatgccgaa ggtgccgagc agcacgacgg gcaccgcgat cgcggggatc
     2101 agcaccgcgc gccagctttg caggaagacg aacatcacga ggatcacgag caggatcgcc
     2161 tcgaacagcg atttctgcac ctcgctgacc gacagcttga tgaaggcggt cgaatcgttg
     2221 gaataagagt aggtgagccc gtcggggaaa tcggcggcga gttcggtcat ccgcgccttg
     2281 acgcggtcgg cggtctcgag cgcatcggat cccggcgaca gcgagatcga catgcccgat
     2341 ccgggatggc cgttgatgcg gacgatggag ctgtaatttt cggcgccgat ctcgacccgc
     2401 gcgacatcct tgatccgcac cgtcgcgccg tcgggcagcg tcttcagcac gatattctcg
     2461 aattgttcgg cggtttgcat ccgcgactgg gcggtgaccg tcgcgttgag catctgaccc
     2521 tcgggcgcgg gcaggccgcc gacctcgccc gccgcgacct cgctgttctg cgcgctgatc
     2581 gccgaaacca cgtcgctcgg catcagcgac atcgccgcga gccgctgcgg gttcagccag
     2641 atacgcatcg cgtgcggcga tccgaagacg ttgacgtcgc cgacgccttc gacgcgcgac
     2701 agcgggtcct ggatgttcga cgacagatag tcggacacgt cctggttcga ccgcgtgtcg
     2761 gtcgtgtcat agacgccgac gagcagcagc gaatccgagt tggatttggt gacgcgcacc
     2821 ccctgcgcct gcacctgctg cggcaggcgc gagaccgccg actggatctt gttctggacc
     2881 tgcacctgcg cgatgtcggg gtcggtcccc ttcacgaaga tcgcggtgat gtccgcgcgc
     2941 ccgcgcgagc tcgattgcga gctgaaatag aggaggccgt cgatcccggt cagctgctgt
     3001 tcgagcacct gcgtgacgct gttctcgatc gtttcggccg atgcgccggg ataagaggcg
     3061 cggatattga cctgcgtcgg cgcgatgtcg ggatattgct cgatcggcag cgaaaagagc
     3121 gcgccgacgc cgccgagcat gacgatgatc gccagtaccc aggcaaagat cggccggttg
     3181 atgaaaagac gcgacatgac gcgctacttg cccttcgccc cggcgccttc gcccttcggc
     3241 gcgccgacgc gctgcgcgct gctcgccggc actgggcgga ccgccgcgcc ctgccgcagg
     3301 ttgccgatcc cctgcgagat gacgcgttcg ccgggtttca gcccgtcggt gacgacccag
     3361 ttggcacccg acgtgcggtc ggcgacgatc ttgcgccgca ccgccttatt gtccttgccg
     3421 accagataga cgaaggccga accgtcgaaa tcgcgttgta ccgccggctg cggcaacagg
     3481 atcgccgacg ggttcaccgc ctgatcgaac atcgcggtca cgaacatgcc gggcagcaac
     3541 acgccctttg gattgggaaa acgcgcgcgc agcgtcacgg taccggtcgc ttcgttgacc
     3601 gtgacgtccg aaaactggac cgtcccggca aagccataat tgctgccatc ctcgaggcgc
     3661 aggcgcaccg acgtgctgcc ggcgttgacc ccgccgcttt cgatcgcctg gcgcagcgcg
     3721 gtgagctcgg cgctcgactg ctgcatatcg acgaacatcg gatcggtttg ctggatcacc
     3781 gcgagcgggg tcgcctggct cgcgctgacc agcccgcccg gcgtcacgag gctgcgtccg
     3841 atgcggccgc tgatcggcgc ggtgatcgtc gcgaagcgca ggttgat
//
LOCUS       DATMSU010000471         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_121956,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000471 DATMSU010000000
VERSION     DATMSU010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121956"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(190..1548)
                     /locus_tag="VN034_12530"
     CDS             complement(190..1548)
                     /locus_tag="VN034_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWV61480.1"
                     /translation="MAKNASFSARRAASLLLPLTLLAGPAFAQTEVKEDSVILQPDKV
                     RDDAPVVAVRIDMPNWTEENATALLSFIEKVGDEGLFARDYNPDGLAAAILGGDQAAL
                     DKVATDSFLLLATHLRDGRTPNAARKQWFMVDSDGDAEPLLSLLTAALGAGTVPETLA
                     SLDPVHPDFAVLKASLQTAKTPAAANAIRVNMERWRWMPRDLGERYVVSNVPEYLTRV
                     VHGGTTIATHKAVVGKNSTPTPQLNPMATGIIVNPTWTLPRSIINEGIGATIARNPSA
                     ARAQGYTWTGKGNTLSVVQQPGANNALGVMKMEMLNEHAIYLHDTPSKGAFNAAARAF
                     SHGCIRTERALHFSGLMAVMFAGKSPEEFGEAIASGKTTRFGFDQPFPVYVAYWTMVP
                     DGKGGVKKLADLYGRDAPVVASFAKPGRPTATIIAPEPPPVVPTIPTAARVTTPETSG
                     IY"
     gene            complement(1776..3137)
                     /gene="accC"
                     /locus_tag="VN034_12535"
     CDS             complement(1776..3137)
                     /gene="accC"
                     /locus_tag="VN034_12535"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWV61481.1"
                     /translation="MAIEKLLIANRGEIALRIHRACHEMGIKTVAVHSTADTDAMHVR
                     LADEAVCIGPPAAKDSYLNIPAIIAAAEITGADAIHPGYGFLSENERFAEIIEAHNMV
                     FVGPKPEHIRTMGDKVEAKRTAVKLGLPVVPGSPGAVTFGDETKKLAKDIGYPVLIKA
                     ASGGGGRGMKVVPDEDSLESLMGQASSEAAAAFGDPTVYMEKYLGNPRHIEFQVFGDG
                     KGNAIHLGERDCSLQRRHQKVLEEAPSPIISAEERARMGGICADAMAKMGYRGAGTIE
                     FLWENGEFFFIEMNTRIQVEHPVTEMITGFDLVREQIRIAGGAGLSVKQEDLEFRGHS
                     MECRINAEDPRSFLPSPGKVTSYHAAGGMHVRVDSGLYAGYSIPPYYDSMIGKLIVYG
                     RSRESCMMRMRRALEEMVIGGVKTNIPLHQALLADPDVIHGDYTIKWLEEWLARQDEE
                     AKA"
     gene            complement(3147..>3441)
                     /locus_tag="VN034_12540"
     CDS             complement(3147..>3441)
                     /locus_tag="VN034_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWV61482.1"
                     /translation="AAPAAPAAAPAAAAPAADNFADAVKSPMVGTVYLAPEPGAPNFA
                     AIGAAVKPGDTILIIEAMKVMNPITATASGTLKAVHVENSQPVEFDQPLFTIG"
BASE COUNT          552 a         1090 c         1153 g          646 t
ORIGIN      
        1 gttgaagccg cccgtgcagc catggcagca accggtcgtt ttcagaccgt cctcaattcc
       61 cgttcgtgtc gagcgaagtc gagacaccca tcgacgtagc acttcgtccg aggggcatct
      121 cgacttcgct cgatgcgaac gagggtttgg aaacgcgggc cggagttttc gctcgaaacc
      181 ctatgccgct caataaatcc cgctggtctc cggcgtcgtc acgcgcgcgg cggtggggat
      241 cgtcgggacg accggcggcg gttcgggcgc gatgatcgtc gcggtcgggc ggccgggttt
      301 ggcgaagctc gccaccacgg gcgcgtcgcg gccatagagg tcggcgagct tcttcacgcc
      361 gcccttgccg tcgggcacca tcgtccaata ggcgacataa acgggaaagg gctggtcgaa
      421 gccgaagcgc gtcgtcttgc cgctcgcgat cgcctcgccg aactcctcgg ggcttttgcc
      481 cgcgaacatc accgccatca ggcccgagaa gtgcagcgcc cgctcggtgc ggatacagcc
      541 gtggctgaac gcgcgcgcgg cggcgttgaa cgcgcccttc gagggggtgt cgtgaaggta
      601 gatcgcatgc tcgttgagca tttccatctt catcacgccg agcgcattgt tcgcccccgg
      661 ctgctgcacc accgacagcg tgttgccttt gcccgtccag gtatagccct gcgcgcgcgc
      721 cgccgacgga ttgcgcgcga tcgtcgcgcc gatgccttcg ttgatgatgc tgcgcggcag
      781 cgtccaggtc gggttgacga tgatgcccgt cgccatcgga ttgagctgcg gcgtcggggt
      841 cgaattcttg ccgacgacgg ccttgtgcgt cgcgatggtc gtgccgccat ggacgacgcg
      901 ggtcagatat tcggggacgt tgctgacgac atagcgttcg ccgaggtcgc gcggcatcca
      961 gcgccagcgt tccatgttga cgcggatcgc attggccgcg gcgggggtct tcgccgtctg
     1021 gagcgacgcc ttgagcaccg cgaaatcggg atgcaccgga tcgaggctgg cgagcgtttc
     1081 gggcaccgtt ccggcgccga gcgcggcggt gagcagcgaa agcaggggtt cggcgtcgcc
     1141 gtcgctgtcg accatgaacc attgtttgcg cgcggcattg ggggtccgcc cgtcgcgcag
     1201 gtgcgtcgcg agcagcagga agctgtcggt cgcgaccttg tcgagcgccg cctgatcgcc
     1261 gccgaggatc gccgcggcga gaccgtcggg attgtaatcc ctcgcgaaca ggccttcgtc
     1321 gccgaccttt tcgatgaagc tcaaaagcgc ggtggcattt tcctcggtcc agttcggcat
     1381 gtcgatccgc accgcgacga cgggcgcgtc gtcgcgcacc ttgtcgggct ggaggatcac
     1441 cgaatcctcc ttcacctcgg tttgcgcgaa ggcgggcccc gcgagcaggg tcagcgggag
     1501 aaggagagac gccgcgcgac gcgcggaaaa agaagcgttt ttggccatgg tcaaagccca
     1561 tagccaaaaa gcggggccag cgaaagtgag gcggagcaca gctcggcgca cgatcccaaa
     1621 tgccctctcc ccttcagggg agaggatatg aaggcttgcg agcttgctcg ctagccgaag
     1681 ttggagaggg gaaatattcc gcccctctcc aagctgcgct agctcctgtg gagcaagctt
     1741 cgctatcctc tcccctgaag gggagagggc gtgactcagg ccttcgcctc ctcatcctgc
     1801 cgcgccagcc attcctcgag ccatttgatc gtataatcgc cgtggatgac gtcggggtcg
     1861 gcgagcaagg cctggtggag cgggatgttc gtcttgaccc cgccgatcac catttcctcg
     1921 agcgcgcggc gcatccgcat catgcagctt tcgcggctgc ggccatagac gatcagcttg
     1981 ccgatcatgc tgtcgtaata gggcgggatc gaatagccgg cgtagagccc gctatcgacg
     2041 cgcacgtgca tgccgcccgc cgcgtgatag ctcgtcacct tgccgggcga gggcaggaag
     2101 ctgcgcggat cctcggcgtt gatgcggcat tccatcgaat ggccgcggaa ttcgaggtct
     2161 tcctgtttga ccgacagccc cgcgccgccc gcgatgcgga tctgttcgcg gacgaggtcg
     2221 aaaccggtga tcatctcggt caccggatgc tcgacctgga tgcgcgtgtt catctcgatg
     2281 aagaagaact caccattttc ccacaggaac tcgatcgtgc cggcaccgcg atagcccatc
     2341 ttggccatcg cgtcggcgca aatgccaccc atgcgcgcgc gttcctcggc cgagatgatc
     2401 ggcgacggcg cttcctcgag caccttctgg tgccggcgct ggagcgagca gtcgcgctcg
     2461 cctaggtgaa tcgcattgcc cttgccatca ccgaagacct gaaattcgat atggcgcggg
     2521 ttgccgagat atttttccat ataaacggtg gggtcgccaa aggcggccgc cgcttcggat
     2581 gaggcctgcc ccatcaggct ttcgaggctg tcctcgtcgg gaacgacctt catgccgcgc
     2641 ccgccgccgc ccgacgcggc cttgatcagc accggatagc cgatgtcctt tgcgagcttc
     2701 ttggtttcgt cgccgaaggt caccgcgccc ggcgaacccg gaacgaccgg aagaccgagc
     2761 ttgaccgcgg tgcgcttcgc ctcgaccttg tcgcccatcg tgcggatatg ctcgggcttc
     2821 ggcccgacga ataccatatt atgcgcttcg atgatctcgg cgaaccgctc gttttcggaa
     2881 aggaagccgt aacccgggtg gatcgcgtcg gcgccggtga tctcggccgc cgcgatgatc
     2941 gcggggatgt tgagatagct gtccttcgcc gcgggcgggc cgatgcagac ggcttcgtcg
     3001 gcaaggcgga cgtgcatcgc gtcggtgtcg gcggtcgaat gcaccgcgac cgtcttgatg
     3061 cccatttcgt ggcacgcgcg gtggatgcgc agcgcgatct cgccgcggtt ggcgatcagc
     3121 agtttctcga tggccatggg ctgaatttac ccgatggtga acagcggctg gtcgaactcg
     3181 accggctggc tgttctcgac atgcacggcc ttgagcgtgc ccgacgcggt cgcggtgatc
     3241 gggttcatca ccttcatcgc ctcgatgatc aggatcgtgt cgccgggctt caccgccgcg
     3301 ccgatggccg cgaaattggg cgcgccgggt tcgggcgcga gatagacggt gccgaccatc
     3361 ggcgatttca ccgcgtcggc gaaattgtcg gcggcagggg ccgctgccgc aggggcggcc
     3421 gccggagcag cgggggctgc g
//
LOCUS       DATMSU010000472         1625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_102656,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000472 DATMSU010000000
VERSION     DATMSU010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102656"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VN034_12545"
     CDS             <1..457
                     /locus_tag="VN034_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HWV61483.1"
                     /translation="AFAEMGGLTGRGARDRVEVPGGHILVIDESYNANPASMAATIGQ
                     LGTESAGRKVAILGAMKELGPDGEAYHAALAAPLVAAGVQFALLVGEEMAPLAKALEG
                     RIDFEHVPAHSAAVGRLGDLIRAGDAVLVKGSNSVGLLHVVTALTSGDY"
     gene            457..1527
                     /gene="mraY"
                     /locus_tag="VN034_12550"
     CDS             457..1527
                     /gene="mraY"
                     /locus_tag="VN034_12550"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWV61484.1"
                     /translation="MLYWLAEWLGFPGALNLIRYLSFRSGAAVATAMILGLWIGPRFI
                     LMLRMRQGKGQPIRDDGPQSHLAKKGTPTMGGLMILISLMISALLWMDLSNRFVWACL
                     FVSAGFAVVGFLDDYDKVTKASHRGIPGRVRLLIEFLIAGVAVLLIVSRTGTDLYLPF
                     FNNVYIPLGPLYYVFAMVLIVGFGNAVNLTDGLDGLATFPVIIASLTFLVIVYLSGNV
                     KFAEYLGIPHVPGAGELAVFAAAIIGACLAFLWFNAPPAAVFMGDTGSLALGGALATI
                     AVTAQHELVLVLVGGLFVVEALSVIIQVFWYKRTGKRVFRMAPIHHHFEQLGWPESTV
                     VIRFWIVSIVLALAGLATLKLR"
BASE COUNT          228 a          550 c          521 g          326 t
ORIGIN      
        1 cgcctttgcc gaaatgggcg gccttaccgg acgcggggcg cgcgaccgcg tcgaggttcc
       61 gggcggacac atcctcgtca tcgacgaaag ctataacgcc aaccccgcct cgatggcggc
      121 gacgatcggc cagctcggga ccgaatccgc aggccgcaag gtcgcgattc tcggcgcgat
      181 gaaggagctc ggcccggacg gcgaggccta tcacgccgcg ctcgccgctc cgctcgtcgc
      241 ggcgggagtg caattcgccc tgcttgtcgg cgaagagatg gcgccgctcg ccaaagcact
      301 tgagggccgc atcgatttcg agcatgtgcc cgcccactcg gccgcggtag ggcggctcgg
      361 tgacctgatc cgcgccggcg acgccgtgct ggtcaaaggg tcgaacagcg tcggcctgtt
      421 gcatgtcgtg acggcgctga ccagcgggga ttattgatgt tatattggct ggcggaatgg
      481 cttggctttc cgggggctct caacctcatc cgctacctga gcttccgctc gggcgccgcg
      541 gtcgcgaccg cgatgatcct cgggctgtgg atcgggccgc gcttcattct gatgctgcgg
      601 atgcggcagg ggaagggaca gccgatccgc gacgacggcc cgcaaagcca tctcgccaaa
      661 aagggcacgc cgacgatggg cgggctgatg atcctcattt cgctgatgat ttcggcgctg
      721 ctctggatgg atctctccaa tcgcttcgtc tgggcgtgcc tgttcgtctc cgcgggcttc
      781 gcggtggtcg gctttctcga cgattacgac aaggtgacca aggccagcca ccgcggcatt
      841 cccggccgcg tgcgcctgct tatcgaattc ctgatcgcgg gggtcgcggt gctgctcatc
      901 gtctcgcgca cgggcacaga cctttatctg cccttcttca acaacgtcta tattccgctc
      961 ggcccgctct attatgtctt cgcgatggtg ctgatcgtcg gtttcggcaa cgcggtgaac
     1021 ctcaccgacg ggctcgacgg gctcgcgacc tttccggtga tcatcgccag cctgaccttc
     1081 ctcgtcatcg tctatctctc gggtaacgtg aagttcgccg aatatctcgg catcccgcat
     1141 gtccccggcg cgggcgagct cgcggtattc gccgccgcga tcatcggcgc ctgcctcgcc
     1201 ttcctctggt tcaacgcgcc ccccgccgcc gtcttcatgg gcgacacggg cagcctcgcg
     1261 ctcggcggcg cgctcgcgac gatcgcggtg accgcgcagc atgaactggt gctcgtcctc
     1321 gtcggcgggt tgttcgtcgt cgaggcgctg tcggtgatca tccaggtctt ctggtacaaa
     1381 cgcaccggca agcgcgtctt tcgcatggcg ccgatccacc atcatttcga gcaattgggc
     1441 tggcccgaat cgaccgtcgt catccgcttc tggatcgtct cgatcgtcct cgcgctggca
     1501 gggctcgcga ccttgaaact ccggtgatca cctcgcgcgc ctttgccggt cgccgctacg
     1561 cggtgctggg actggcgcgc tcgggcctcg cgaccgtcga ggcgctcgtc gccagcggcg
     1621 caggc
//
LOCUS       DATMSU010000473        11706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_149757,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000473 DATMSU010000000
VERSION     DATMSU010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11706
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149757"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VN034_12555"
     CDS             <1..1389
                     /locus_tag="VN034_12555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWV61485.1"
                     /translation="GVNIWQAAWCGPHHIAAICSDQPEENWWYTADVRLIAIDGGAAR
                     TLFKPADQLGWLAVSPSGATVAVVEAVCSDRNIVAGDVRLIDVASGTVDDPPSPGGDV
                     VQLHWRDDDRLLFVAAQGPDTLVGLIDRKTKNTGTLWQGGQRTPSGTMFPEVAPLGSN
                     PADFLFQIESFFDPPTLFAFENGQEREVRRFGSPETDAIVRPLGTARDFRWTAPDGLE
                     IHGWLITPPGDGPHPLIMQVHGGPVWYTRPLYIGRSAFAQMALAEGYALFQPNPRGSS
                     GRGQAFARHVFGDMGGADTHDYLSGLDALEKAGIADPRRIGVTGGSYGGYISNWLITQ
                     DQRFAASVPVAPIANWVSQHFTCNVPSFCQMFLNDHVSNPTGKYFAHSPVHHAARVKT
                     PTLNICGALDHITPPGQALEFHRALQELGVESLLLTYPTEGHGVRTMPAIFDFNARVM
                     AWFLKHMPPDRD"
     gene            1432..3549
                     /locus_tag="VN034_12560"
     CDS             1432..3549
                     /locus_tag="VN034_12560"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61486.1"
                     /translation="MTRLFLTLMTSTALLPAAANAQAAADAPDAQPTDIIVTAQRYEQ
                     RLQDVPLSISAIGAEELRARGTTDLKDLQYSVPGLSTFEYGVSRQFVQLRGMATTIGS
                     STVGVYLDETPLGLDAQGDPMNVRLYDMERVEVLRGPQATLYGQGSMGGTIRYIPAAP
                     KLDAVGGSFEGEYSSTRHGDSNYKAVGMLNLPVATDKLGVRLVAGYERIGGFIDNVAT
                     GEDDINAADIYTLRGTLLAQPTERLSLSLMAVYQKSEQDSQDFGVGYETTALLPQPIE
                     DRYTLIQGKAGYDLDFAELSVSASYIDRENNSVSDVSGFYVPFLGLLGFPPGFIDRVG
                     LANASDFRIYNGEIRLVSQSDGPLGWQVGALYRDSRSHQVSGAFTEPNAAPIILSASD
                     VLYRNKSHAFYGELNYAFTPKLKAIVGLRYFNEHKKVDIDSTNLGASSIDIGDESFNS
                     LNPRFNLSYEFSPSSMVYANVAKGFRSGGFNATSVGGGVIDIPPTYDSDEIWTYELGT
                     KHQLFDGKLALEASVYRNEWSKVQSYTFVPGTALAAVTNSGHVEGWGVDLSAMARPTR
                     DLTLSATYGWNNLKFDKATVDKAVGDPVDGAVRESWSASLDWRPALTAVIEGIFRIDY
                     QHAGASQITLRSFNVPPVIPRPGRDLVNLRIGVAAGPVEIALFANNLFDENAPNLIGP
                     LGTIAENIEQRPRVIGIGANARF"
     gene            complement(3613..4218)
                     /locus_tag="VN034_12565"
     CDS             complement(3613..4218)
                     /locus_tag="VN034_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61487.1"
                     /translation="MAGRTPKNTASDWIDAGQRMLVDEGIAGLKVDRLASRLGVSRGG
                     FYHHFRDRDEFLDRLVGHWEATCRFLPDTPPPARPAEAVAWFDGTIERLIESDGYDYH
                     FDLAVREWARADTRVAWAVERADRERIETLRKFFEAIGYAGEEAATRARIFYYHQIGY
                     YAIGVRQSIVERRRNAELYIDILCGEQALAAARTTWKARAA"
     gene            complement(4405..5670)
                     /locus_tag="VN034_12570"
     CDS             complement(4405..5670)
                     /locus_tag="VN034_12570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61488.1"
                     /translation="MLIATTLFAFVDRQIINLIAPSLQKDLGISDLQLGIMHGLGLAL
                     FASAAAYPIGWLADRYGRRLVLGCCILVWSASTAACAFQTSFAGIFAATAGIAIGEAA
                     LTPVIFSMLPDLFPERQRNTANFIFFGVALLGVAVGLGLGGVTLELLENSHQSLPAIF
                     SGLESWRIALILVATPGPLFVLLLMTIRMKRNAGRTGGEDGSGDAIGIGKMLPFLRAH
                     WRTIACIYGAIFVYGMPLGGALSWLPVAMPRAFKAVETSSVGIQMGIAIGIGSIVGLI
                     MPALGIKLLGGDTDLKPLRLARVFLAVASLPMLFLLVAWEPWQVYAAAGVQLACLLAT
                     GALMPGVLQNLAPPDLRSRMLSLLGICGAISGGLAPMLVGALSGLLDAQQGLFTAMVI
                     VGVPAWLLAAALMSMARRPYMGMIAALRV"
     gene            complement(5810..7942)
                     /locus_tag="VN034_12575"
     CDS             complement(5810..7942)
                     /locus_tag="VN034_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61489.1"
                     /translation="MTKWIAMLMAGTAVLPTIAYAQDAVSGAGDESGESQPGDIIVTA
                     QRYEQRLQDVPLSISVVGAEELQGRGVTALTDLQYSVPGLSIYEVGTGKQTVQLRGIS
                     TSIGSTTVGIYYDETPLSLDTTGDSFMVRMIDLERVEVLRGPQATLYGNGSMGGTIRY
                     IPAAPRLDAVSGSANVEYNQTRYGEAGYKAVGVINLPLATDVAAVRLAAAYERTGGFI
                     DNVATGEKDINRTDIYTVRGSLLVKPTEELSISLLGLYQKSKQPSQDIGIDYQTVMPV
                     PSPLRDRYTLIQGKVGYDLGFAELSASGSYIDRRNFTQFDLSALFVPLIGALGFPPGY
                     ITEVPTLATTDFKVWNSEVRLSSQGDGPFGWQVGVAHNDLKVHQFGGTETAPGALPFP
                     IIASDDRRRSKAWTIYGEASYTVTPELRATAGLRWFSENKQRVIDSTNFGVTSLDTGD
                     KTYRTVNPRFNLSYDFTPDSMVYINVAKGFRSGGFNQTSAGGGVIPVPPTYKPDSIWT
                     YELGTKHALAGNKLTLDASVYRSEWSDVQSNNFAPGNVITIVNNSGHVSGWGVDLSVG
                     ARPTRDLTLSATYGWNNLKYDEATADKAVGDPVDAAVRESWSASADWRPALSDSVNGI
                     VRLDYQHAGRGQFTLRNLNPVLIVARPKRDLVNLRVGAAFGPVEVALFANNLFDENVP
                     VVVGPFGLILENVEQRPRVIGISASTNF"
     gene            complement(8086..10146)
                     /locus_tag="VN034_12580"
     CDS             complement(8086..10146)
                     /locus_tag="VN034_12580"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWV61490.1"
                     /translation="MTDITTPLAYPATERRPEAVEFGPVRYDDAFRWLEEDADTAVAE
                     WQRAQDALARAYLAGLPASESFAGRIAAIGETDDVTIPTFAGGRWFKRYVPDGQDLAV
                     VEVGDSPTEAGRRLIDLNAMRTGEPLSLGVFLPSPDGRKAIFSWAAGGREEPDLQILD
                     IDSGDVLVDGVPHHKPQNFAWLPDSSGFIYLALDAALAGKPSLYRVNLADPAVIAEDV
                     SVDHPVVRPIPSADRRHIVLAVDHLAPRLDYILDTEGDAGWQPFLKGVPGIFRGDILG
                     DHFLAITDDGAPRGRLVSIPLATPTQRETWKEIVPASDNVLAFVLVTGERAVLLEWID
                     TYSRLRVIGKTGEIEGEITLPGRGMVNSFGANMVLPTMIDPIARGAEGEILFVFSAFD
                     QSPALYSANLARRETKQLGEPKCRLDLQVRDLECRSADGARVLYHVVARADLDLSKPQ
                     PTVITGYGGFNVTVAPGWLGTQWAAWIEAGGVVALAHLRGGGEHGTPWFEQGRMKLKQ
                     NSFNDVHAIAEDLGARGISASDRLGVTGGSNGGVMASAVAVQRPDLYRASIPVVPITD
                     VLGRVRDPVTMVSSLDYGDPADPEMAEAIHSWSPYQNVRDNIAYPALLIDCGANDPRC
                     PPWHGRKFAARVQQASNGGLPTLLRVRADAGHGAVGKAQKVRMSADNLAFFAHQLGLT
                     VEGA"
     gene            complement(10180..>11706)
                     /locus_tag="VN034_12585"
     CDS             complement(10180..>11706)
                     /locus_tag="VN034_12585"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWV61491.1"
                     /translation="RERRDRLDRIDLASFDRTDRLTAGFVRHWLDIAIDEPQRWWTGF
                     GIAPYSGIWLSLVPRMLFAPIDLTDAAETARYLKLAEEFAAAVDAMRERVLAQAERGW
                     RLPKPALPNARKTLEGNASMAVAAVLLSDDRAASAEIRAAVTKIVEEQLKPAFARLLD
                     TIGPDYEAAAPEAAGMMHQPGGAEAYRLWLRYHLGAELDPADLHQLGLDEVATLAAEM
                     ERVRTEQFGHNGDEASFHERLRGDPKAKAPSAEALEATYARHLDRMAPVFARMFRRAP
                     QSPAAVRRLPRELEAGMTFGYYQTPAEVGAEGVYYYSGNGIPDRMQMNAAALIFHELV
                     PGHHVHLSRQQENGALPEIRRNMPISTAFNEGWAEYASGLGEEAGLFDNPYDYYGFLS
                     HQRFVAQRLVVDTGLNALGWTLDEAHAYMSANTLEKPEQVTSDILRYATDLPGQALCY
                     RWGCLKFRELRAKAEAQLGAKFDLADFHEAILDQGCLPIPVLEESLDQWARERALADG
                     "
BASE COUNT         2068 a         3813 c         3806 g         2019 t
ORIGIN      
        1 ggcgtcaaca tctggcaggc cgcgtggtgc gggccgcacc atatcgccgc gatctgctcc
       61 gaccagcccg aggagaattg gtggtacacc gccgacgtcc ggctgatcgc aatcgacggc
      121 ggcgccgcgc gcaccctgtt caaacccgcc gaccaactcg gctggctcgc cgtatcgccg
      181 tcgggcgcaa cggtcgcggt ggtcgaggcg gtgtgcagcg accgcaacat cgtcgcgggg
      241 gacgttcggc tgatcgatgt cgcgagcggc acggtcgacg acccgccctc gccgggcggc
      301 gacgtcgtcc aactgcattg gcgcgatgac gaccggctgc tgttcgtcgc ggcgcaggga
      361 cccgatacgc ttgtcggact tatcgatcgc aagacgaaaa acaccggcac gctctggcag
      421 ggtggccagc ggaccccctc gggcaccatg tttcccgagg tcgcgccgct cggcagcaac
      481 cctgcggatt tcctgttcca gatcgaatcc ttcttcgatc cgccgacgtt gtttgcgttc
      541 gaaaacgggc aggagcgcga agtccgccgg tttggatcgc cggagaccga cgcgatcgtt
      601 cgccctttgg ggacggcgcg cgatttccgg tggacggcgc ccgacggcct cgaaatccac
      661 ggctggctga tcaccccgcc gggcgatggc ccgcatccgc tgatcatgca ggtccacggc
      721 ggcccggtat ggtatacgcg gccgctctac atcggccgct cggccttcgc gcagatggcg
      781 ctcgccgaag gttatgcgtt gttccagccc aatccgcgcg ggtcgagcgg gcgcgggcag
      841 gcgttcgcgc ggcacgtgtt cggcgatatg ggcggcgccg atacccatga ttatctctcg
      901 ggcctcgatg cgctcgaaaa agcgggcatc gccgatcccc ggcgcattgg cgtcacaggc
      961 ggcagctatg gcggctatat cagcaactgg ctgatcacgc aggaccagcg cttcgcggcg
     1021 tcggtgccgg tcgcgccgat cgccaattgg gtcagccagc atttcacctg caacgtcccg
     1081 tccttctgcc agatgttcct gaacgatcat gtgagcaatc cgacgggcaa atatttcgcg
     1141 cacagtcccg tccaccacgc ggcccgcgtg aagaccccga cgctcaatat ctgcggcgcg
     1201 ctcgatcata tcacgccgcc ggggcaggcg ctggaattcc atcgcgcgct gcaggaactg
     1261 ggcgtcgaaa gcctgctcct gacttacccg accgaggggc acggcgtccg caccatgccc
     1321 gcgattttcg acttcaacgc ccgggtgatg gcctggttct tgaaacatat gccgcccgat
     1381 cgggactgat cgccgacgac agacaaaata atagaattgg ggagttcgac catgaccaga
     1441 ttgttcctga ccttgatgac gagcaccgcg ctgctgccgg ccgccgcgaa tgcgcaggcg
     1501 gccgccgatg cgcccgatgc gcagccgacc gacatcatcg tcaccgccca gcgatacgaa
     1561 cagcggctgc aggatgtccc cctgtcgatc agcgccatcg gtgccgaaga gctcagggcg
     1621 cgcgggacga ccgacctcaa ggatctccaa tattcggtcc ccggcctttc gaccttcgaa
     1681 tatggcgtca gccgccagtt cgtgcagctg cgcggcatgg cgacaacgat cggttcgtcg
     1741 accgtcggcg tctatctcga cgaaaccccg cttgggctcg acgcgcaggg cgacccgatg
     1801 aatgtccgcc tctacgacat ggaacgcgtc gaagtgctgc gcgggccgca ggcgacgctc
     1861 tatggccagg gatcgatggg cggcacgatc cgctacatcc ccgccgcgcc caaactcgat
     1921 gccgtcggcg gatcgttcga gggcgaatat tcctccacgc gccatggcga cagcaattac
     1981 aaggcggtgg gtatgctcaa cctgccggtc gcgaccgaca agctcggcgt gcggctggtg
     2041 gcgggctatg agcggatcgg cggcttcatc gacaatgtcg cgaccggcga ggacgacatc
     2101 aacgccgccg acatctacac gctgcgcggc acattgctcg cgcagccgac cgagcggctc
     2161 agcctgtcgc tgatggcggt ctatcagaaa tcggagcagg acagtcagga tttcggggtc
     2221 ggctatgaaa ccaccgcgtt gctgccccaa ccgatcgagg accgctacac gctgatccag
     2281 ggcaaggcgg gctacgacct cgacttcgcc gaactgagcg tgtcggcgag ttatattgac
     2341 cgcgaaaaca acagcgtttc ggacgtctct ggcttctatg tgccttttct tggcctgctt
     2401 ggcttcccgc cgggcttcat cgaccgcgtc ggcctcgcga acgcgtccga ttttcgtatc
     2461 tacaatggcg aaatccggct ggtctcgcaa agcgacggac ccctcggctg gcaggtcggc
     2521 gcgctctatc gcgattccag atcgcatcag gtttcggggg cgttcaccga gcccaatgcg
     2581 gcgccgatca tcctttcggc atccgacgtg ctgtaccgga acaaatccca tgccttttac
     2641 ggcgagctga actacgcctt cacgccaaag ctcaaggcca tcgtcggcct gcgctatttc
     2701 aacgagcaca agaaggtcga tatcgacagc accaatctgg gcgcctccag catcgatatc
     2761 ggcgacgaaa gcttcaactc gctcaacccg cgcttcaacc tcagctacga attctcgccg
     2821 agctcgatgg tctatgcgaa cgtcgcgaag ggcttccgca gcggcggttt caatgcgacg
     2881 tcggtcgggg gcggcgtcat cgacattcca cccacctatg actccgacga aatctggacc
     2941 tacgagctcg gcaccaagca ccagcttttc gatggcaaac tggcgctcga agcgagcgtc
     3001 tatcgcaacg aatggtcgaa ggtgcagtcc tacactttcg ttccggggac cgcgctcgcg
     3061 gcggtcacca attcgggcca tgtcgagggg tggggcgtcg atctgtcagc gatggcacgg
     3121 ccaacgcgcg acctgactct ctccgcgacc tatggctgga acaacctcaa gttcgacaag
     3181 gccaccgtcg acaaggcggt cggcgatccg gtcgacggcg cggtgcgcga atcctggtcg
     3241 gcgtcgctcg actggcggcc tgcgctgacc gccgtcatcg agggcatctt ccgtatcgac
     3301 tatcagcatg ccggcgcgtc gcagatcacc ctgcgcagct tcaacgtgcc gccggtgatc
     3361 ccgcgtccgg gccgcgacct cgtcaatctg cgcatcggcg tcgcggcggg gccggtcgaa
     3421 atcgcgctgt tcgcgaacaa tttgttcgac gaaaatgccc cgaacctcat cggcccgctt
     3481 ggcacgatag ccgagaatat cgagcagcgt ccgcgggtga tcgggatcgg cgcgaacgcg
     3541 aggttctgag ccccttcggg ggcggggcac cgggcggcgg cggctccttg caagaggcgg
     3601 ccgcccggtc gttcaggcgg cgcgggcttt ccatgtggtg cgcgccgcgg ccaaggcctg
     3661 ctcgccgcag aggatgtcga tgtaaagctc cgcattgcgg cggcgttcga caatgctctg
     3721 ccggacgccg atcgcgtaat agccgatctg gtggtaataa aagatgcgcg ccctcgtcgc
     3781 tgcctcttcg cccgcatagc cgatcgcttc gaaaaatttc cgcagggttt cgatccgctc
     3841 gcggtcagcg cgctcgaccg cccatgcgac gcgtgtgtcg gcgcgcgccc attcgcgcac
     3901 cgcgaggtcg aaatgatagt cgtaaccgtc ggattcgatg aggcgctcga tcgtgccgtc
     3961 gaaccacgca acggcttcgg cggggcgggc gggcggcgga gtgtcgggca gaaaccggca
     4021 cgtcgcctcc caatgcccga ccagccggtc gagaaattcg tcgcggtcgc ggaaatgatg
     4081 gtagaagccg ccgcgcgaca cgccgaggcg gctcgcgagc cggtcgacct tcagcccggc
     4141 aatgccctca tcgacgagca tgcgctgccc cgcgtcgatc cagtcgctgg cggtgttctt
     4201 cggtgtgcgt ccagccatcc gggtttcatt cctgccagtt tgctctgatc gccatcatcc
     4261 tattctttcg ccgaattaca aaggtccgga cggcattctt gggggccgga aacgcacttc
     4321 tcgacgatgg cgaaacgatt ttgtccggcg acgaaagtgc gggaccagct tttcgcaagg
     4381 acccgccttc gaccaggtgc gaaatcagac gcgaagcgcc gcgatcatgc ccatataggg
     4441 gcgccgcgcc atcgacatca gcgctgcggc gaggagccac gcgggtacgc cgacgatgac
     4501 catcgcggtg aacaatcctt gttgcgcgtc gagcaggccg gacagcgcgc ccaccagcat
     4561 cggcgccagt ccgcccgaaa tcgcgccgca gatgccgagc agcgacagca tgcgggagcg
     4621 caggtcgggc ggcgcgagat tctgcaacac gccgggcatc agcgccccgg tggcgagcag
     4681 gcaggcgagc tgcacgccgg cggccgcgta aacctgccag ggttcccacg cgacgagcag
     4741 gaacagcatc ggcaggctgg ccaccgccag aaaaacgcgc gcgagacgga gcggcttgag
     4801 gtcggtatcg ccgccgagga gcttgatccc cagtgcgggc atgatcaggc cgacaatcga
     4861 accgatcccg atcgctattc ccatctgtat cccgaccgac gatgtctcga cggccttgaa
     4921 agcgcgcggc atcgcgaccg ggagccatga gagcgcgccg ccaagcggca ttccataaac
     4981 gaagatcgcg ccatagatgc acgcgatcgt ccgccaatgc gcgcgcagga agggcagcat
     5041 tttgccgata ccgatggcgt cgcccgatcc atcctccccg cccgtccggc cggcgttcct
     5101 tttcatacgg atcgtcatca gcaacaggac gaaaagcggc ccgggcgtcg cgacaaggat
     5161 cagcgcgatc cgccagcttt caaggcccga aaagatcgcg ggcagcgact ggtgactgtt
     5221 ttcgagcagt tccagcgtga caccgccgag accaaggcca accgcgactc caagcagggc
     5281 gacgccgaaa aagatgaagt tcgcggtgtt gcgttgccgt tcgggaaaga ggtcgggcaa
     5341 catcgaaaag atgaccggcg tcagcgcggc ctcgccgatc gcgatccccg ccgtcgccgc
     5401 gaagatgccg gcgaagctgg tctgaaacgc gcaagctgcc gtcgaggctg accagaccag
     5461 gatgcagcag ccgagcacca gccgccgtcc gtaacggtcg gcgagccagc cgatcggata
     5521 ggcagcggcg ctcgcgaaca gcgcaaggcc aaggccgtgc atgatcccga gctgcaggtc
     5581 gctgatgccg agatccttct gcaacgaagg cgcgatcagg ttgatgatct gccggtcgac
     5641 aaaggcgaac agcgtcgtgg cgatcaacac acccagcgca taccaagccg acgcgggggc
     5701 gctttgccga atgccggggg gctgatcact cggcgctgaa agggactcaa gctgggccac
     5761 gcccgtcata aaattgcttt ctcggtgtgc cggcgttccg cagcgacgat cagaaattcg
     5821 tgctcgcgct gatgccgatc acacgcggac gctgctcgac attttcaagg atcagaccga
     5881 aggggccgac cacgaccggc acattttcgt cgaacagatt gttggcgaac agcgccacct
     5941 cgaccgggcc gaaggcggcg ccgacgcgga ggttgacgag atcgcgcttc ggccgcgcga
     6001 cgatcaggac cgggttcagg ttgcgcagcg tgaactgccc ccggccggcg tgctgatagt
     6061 cgaggcggac gatgccgttg acgctgtccg acagcgcggg ccgccagtcg gccgaggccg
     6121 accagctttc gcgcaccgcg gcgtcgaccg gatcgccaac cgccttgtcg gcggtcgcct
     6181 catcatattt cagattgttc cacccatagg ttgcgctcag cgtcaggtcg cgcgtcgggc
     6241 gcgcgcccac cgacaggtcg acaccccagc ccgagacgtg gcccgagttg ttgacgatcg
     6301 tgatgacatt gcccggcgcg aaattgttcg actgaacatc cgaccattcg cttcgataca
     6361 cgctggcgtc gagcgtcagc ttgttgccgg ccagcgcgtg cttcgtaccg agttcgtacg
     6421 tccagatcga gtcgggtttg taggtcgggg gaaccgggat gacgccgccg ccggccgagg
     6481 tctggttgaa gccgccgctg cggaagccct tggcgacgtt gatgtagacc atcgaatcgg
     6541 gggtgaagtc atagctcagg ttgaagcgcg gattgaccgt ccggtacgtc ttgtcgccgg
     6601 tgtcgagcga cgtcacgccg aagttcgtgg aatcgatgac acgctgcttg ttttcgctga
     6661 accagcggag gcccgcggtc gcgcgcagtt cgggtgtgac ggtatagctt gcctcgccat
     6721 agatcgtcca cgccttgctg cggcggcggt cgtcgctcgc gatgatcgga aagggcagcg
     6781 cacccggcgc ggtctcggtg ccgccgaact ggtggacctt caggtcgttg tgcgcgacgc
     6841 cgacctgcca gccgaaggga ccgtcgccct gcgaactcag ccggacttcg ctgttccaca
     6901 ccttgaagtc ggtcgtcgcc agcgtcggga cctcggtgat atagcccggc gggaaaccga
     6961 gcgcaccgat aagcgggacg aacagcgccg acaggtcgaa ctgcgtgaag ttgcggcgat
     7021 cgatatagct gcccgacgcg ctcagctcgg cgaagccgag gtcgtaaccc accttgccct
     7081 ggatcagcgt gtagcgatcg cggagcggcg aggggacggg catgaccgtc tgatagtcga
     7141 tgccgatgtc ctggctcggc tgtttcgatt tctggtagag gccgagcagc gagattgaca
     7201 gctcttcggt cggcttgacg agcaggcttc cgcggaccgt gtaaatgtcg gtgcgattga
     7261 tgtccttctc gcccgtcgcg acattatcga tgaagccgcc ggtgcgctca taggccgcgg
     7321 cgaggcgcac cgcggcgacg tcggtcgcca gcggcagatt gatgacgccg accgccttgt
     7381 agcccgcttc gccgtagcgg gtctggttat actctacgtt ggccgatccg ctcacagcgt
     7441 cgagccgcgg tgctgccgga atataacgga tcgtgccgcc catcgagccg ttgccgtaca
     7501 gcgtcgcttg cggcccgcgc agcacttcga cgcgctcgag gtcgatcatg cggaccatga
     7561 aggagtcgcc cgtggtgtcg agcgacagcg gcgtctcgtc gtaatagatg ccgacggtcg
     7621 tcgagccgat cgaggtcgaa ataccgcgaa gctggacggt ctgcttgccc gtgccgacct
     7681 cgtatatcga aagcccggga accgaatatt gcagatcggt cagcgcggtg acgcccctgc
     7741 cttggagttc ttccgcgccg acgacgctga tcgacagcgg gacgtcctgc agccgctgtt
     7801 cgtagcgctg cgcggtgacg atgatatcgc cgggctgact ttcaccgctc tcgtcgcctg
     7861 cgccgctgac ggcatcctgc gcataggcta tcgtgggaag cacggcggtg cctgccatca
     7921 acatcgcgat ccatttggtc atacagaact ccccaatatt cttatcgttc tcgaacgatc
     7981 ggccccgcta tctttcgagg tttcgaccga aggctaatcc tcgaccgatc cgggcgctta
     8041 gctgaacgtg caaaactatt cggagcgcgt cggaatcggc gtgtttcatg cgccctccac
     8101 ggtcaggccg agctgatggg caaagaaggc gagattgtcc gccgacatgc ggaccttctg
     8161 cgccttgccg accgctccat gcccggcatc ggcgcggacg cggagcaggg tcggcaggcc
     8221 gccgttcgac gcctgctgca cccgcgccgc gaacttgcgg ccgtgccagg gcgggcaccg
     8281 cggatcgttc gcgccgcagt cgatcagcag cgccggatag gcgatgttgt cgcggacatt
     8341 ctgatagggc gaccagctgt ggatcgcctc ggccatttcg ggatcggcgg gatcgccata
     8401 atcgagcgag gagaccatcg tcacaggatc gcgaacgcgc ccgagcacat cggtgatcgg
     8461 cacgaccgga atgctggcgc ggtacaggtc gggacgctgc accgcgaccg ccgacgccat
     8521 gacgccgccg ttcgagccgc cggtgacccc gagccggtcg gaggcactga ttccgcgtgc
     8581 gccgagatcc tcggcgatgg cgtgcacatc gttgaagctg ttctgtttga gcttcatccg
     8641 gccctgttcg aaccacggcg tgccatgctc gccgccgccg cgcagatgcg cgagcgcgac
     8701 gacgccgccg gcttcgatcc acgccgccca ttgggtgccg agccagcccg gcgcgaccgt
     8761 gacgttgaaa ccgccatagc cggtgatcac cgtcggctgc ggcttcgaca ggtcgaggtc
     8821 ggcgcgtgcg acaacgtggt agagcacacg cgcgccgtcg gcgctgcggc attcgagatc
     8881 gcggacttgc aggtcgagcc ggcatttggg ctcgccgagt tgcttcgtct ctcgtctcgc
     8941 gaggttggcg ctatagagcg cgggcgactg atcgaaagcc gagaagacga acaatatctc
     9001 gccctccgcg ccgcgcgcga tcggatcgat catcgtgggc agcaccatgt tcgcaccgaa
     9061 actgttcacc atgccgcggc cgggaagggt gatttcgcct tcgatctctc cggtctttcc
     9121 gatcacacgc aggcgcgaat aggtgtcgat ccattcgagc agcaccgcgc gctcgcccgt
     9181 cacaagcacg aacgcgagca cattgtccga cgcgggcacg atttccttcc acgtctcgcg
     9241 ctgcgtcggc gtcgcgagcg ggatcgacac gagccgcccg cgcggtgcgc catcgtcggt
     9301 gatcgcgagg aaatgatcgc ccaatatgtc gccgcggaag atgccgggca cacccttcag
     9361 gaacggctgc cagcccgcat cgccctcggt atcgaggata tagtcgagcc gcggcgcgag
     9421 atgatcgacc gcaagcacga tatgccggcg gtcggccgac gggatcggcc gcaccacggg
     9481 atgatcgacg ctcacgtctt cggcgatcac cgctggatcg gccagattaa cacgatagag
     9541 cgagggcttc ccggccagcg cagcgtcaag cgcaaggtag atgaaaccgc tgctgtcggg
     9601 gagccaggcg aagttctggg gtttatgatg cggaacaccg tcgaccagca catcgccgct
     9661 gtcgatatcg agaatttgca gatcgggttc ttcgcgtccg cccgcagccc acgaaaagat
     9721 cgccttgcga ccgtcgggcg atggcaggaa aacgccgagg ctcagcggct cgccggttcg
     9781 catcgcatta aggtcgatca ggcggcggcc ggcttcggtc gggctgtcgc cgacctccac
     9841 caccgcgaga tcctgaccat cggggacata tcgtttgaac cagcgtcccc cggcgaaggt
     9901 ggggatggtg acatcgtcgg tttcgccgat cgccgcgatc cgcccggcaa agctttcgga
     9961 cgccggcagt cccgcgagat aagcccgcgc gagcgcgtcc tgcgcgcgct gccattccgc
    10021 gacagcggta tccgcatctt cttcgagcca gcgaaacgcg tcgtcatagc gcacggggcc
    10081 gaattcgacg gcctcggggc gccgctcggt cgcgggatag gcgagggggg tggtgatatc
    10141 ggtcatgtca tcggctttca ttgaatgtcg gatcgtcgct cagccgtcgg ccaaagcgcg
    10201 ctcgcgtgcc cattggtcga ggctttcctc gagcacgggg atcggcaggc agccctgatc
    10261 gaggatcgct tcgtggaaat cggcgaggtc gaatttcgcg cccaactgcg cctcggcttt
    10321 cgcgcgcagc tcgcggaatt tcaggcagcc ccagcgatag caaagcgcct gtcccggcag
    10381 atcggtcgca tagcgcagga tatcgctcgt cacctgttcg ggcttttcga gcgtgttggc
    10441 gctcatatag gcatgcgctt catcgagcgt ccagccgagc gcgttgagcc cggtatcgac
    10501 gacgagccgc tgcgcgacga aacgctggtg cgagaggaag ccgtaataat catagggatt
    10561 gtcgaacagc ccggcctctt cgccgagccc cgaggcatat tcggcccagc cttcgttgaa
    10621 cgcggtcgag atcggcatgt tgcggcgaat ttcgggcagc gctccattct cctgctggcg
    10681 cgagagatgg acatggtggc cgggcaccag ctcgtgaaag atcagtgccg cggcgttcat
    10741 ctgcatccgg tcgggaatac cgttgccgct gtaataatag acgccctcgg cgccgacctc
    10801 ggcgggggtc tggtaatagc cgaaggtcat tcccgcttcg agttcgcgcg gcagccgcct
    10861 taccgccgcg ggcgattgcg gcgccctgcg gaacatgcgc gcgaacaccg gcgccatacg
    10921 gtcgaggtgg cgggcgtagg tggcttccag cgcctcggcc gacggcgcct tcgccttggg
    10981 gtcgccgcgc agccgttcgt ggaacgaggc ctcgtcgcca ttgtggccga attgctcggt
    11041 ccgcacccgc tccatctcgg cggcgagcgt cgccacttca tcgaggccca gctggtggag
    11101 gtcggcggga tcgagctcgg cgccgaggtg gtagcgcagc cacagccgat aggcttcggc
    11161 accgcccggc tggtgcatca tgcctgccgc ttcgggcgcc gccgcctcat aatcggggcc
    11221 gatcgtgtcg agcagccgcg cgaaggcggg cttcaactgc tcctcgacga tctttgtcac
    11281 cgccgcgcgg atctcggcgc tcgcggcccg gtcgtcggaa agcaggacgg ccgcgaccgc
    11341 catcgacgca ttgccttcga gcgtcttccg cgcattcggg agcgcgggct tgggaagccg
    11401 ccagccgcgt tcggcctggg ccagcacgcg ctcgcgcatc gcgtcgaccg cggcggcaaa
    11461 ttcctccgcg agcttcagat agcgcgcggt ttccgccgca tcggtcaggt cgatcggggc
    11521 gaacagcatc cgcggaacaa gcgacagcca gatgcccgaa tagggcgcga taccgaagcc
    11581 ggtccaccag cgctgcggct cgtcgatcgc gatgtcgagc cagtggcgca cgaaacctgc
    11641 ggtcagccgg tcggtgcgat cgaagctggc aaggtcgatg cgatcgaggc ggtcgcggcg
    11701 ctcgcg
//
LOCUS       DATMSU010000474         8492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_26747, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000474 DATMSU010000000
VERSION     DATMSU010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26747"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VN034_12590"
     CDS             <1..454
                     /locus_tag="VN034_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV61492.1"
                     /translation="ARIAPIYEGTNGIQAADLVGRKLGMAGGDLVRGLIDDIAAGTAD
                     SPELQQLVDACRAVTDWMVGANTADRLAGSYPYLTMLATATCGWLMAIQHKAARTALD
                     EGHGDRVFLEAKISSTRFYLQQIVPAATGLAASALAGDAALEPLPAMA"
     gene            661..2952
                     /locus_tag="VN034_12595"
     CDS             661..2952
                     /locus_tag="VN034_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWV61493.1"
                     /translation="MPIPVKSLIISWRTIALALILSVIVGISGAGELPDRMISMATSR
                     VAARPVSGDTVIVALDDKTLQAIGERDFDVNHHTQLVRAIDASPAKRLFIDFSYERRL
                     KDRDFPELAKAVRDMGDRVVLAVPGSSMTGTTAVVDYWPDPAFGTRAQQACICWEYEF
                     WQVWKVPLAVSANGRLLPSFSAKLADRTPDRPKIFALDLSYDPDTITQYSAIDVMTGK
                     LDPRLLRGKDVIFAATAAASSDKHYLPGHDKLPGAYIHLIAGEALKRGNPVDIGWLPA
                     LSFAFVTLLGALAWRSGRWYATTALLLTLALLVAKVVWSLSLVTIQVGPACFLIAAMT
                     ANVSRNRRRSLAQRENPVSGLPNFEALRSQAAFGSATVIAAKVVNFEDLAAFIPGDGI
                     GKLVEQVTRRLQLASQGTTLHHDLDGSFAWLVPYYQHSQIEGQLAGLAALFNAPLTIG
                     ELRVDVAIAFGVNDEFEGSNAQRLAAALVAAERAIRTRSLWTKYTSRQKEDAGWQLSF
                     HSQLEDALTGGDIWVAFQPQYAIATKKLVGVEALARWTHPTRGPIPPDEFIVQAEKSQ
                     DIYRLTLFVMDQAIRSAAQLRERGIHINMSVNLSASLLDHSDLVGTIRVMLTAHHLPP
                     EILTIEITETAQIENSRQARHTLAQLRRTGIRLSIDDYGTGQSNLEYLTEIEADEIKI
                     DKRFVMTMRDSQRNLEVVKSTIDLAHRLGAVAVAEGIEDGETMALLAGLGCDVGQGYH
                     LGKPQLFSELTATLTALPHNRIA"
     gene            3680..5635
                     /locus_tag="VN034_12600"
     CDS             3680..5635
                     /locus_tag="VN034_12600"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HWV61494.1"
                     /translation="MDFRESERVETSDVATMELAKNDAPAAPETKGESAPDSAPAAKL
                     NDGSEPKVHARRFAIVTDASRDALLTDFGKETLQDRYLLPGESYQDLFARVADAYADD
                     QDHAQRLYDYISKLWFMPATPVLSNGGTGRGLPISCYLNSVDDSLEGIVGTWNENVWL
                     ASRGGGIGTYWGAVRGIGEPVGLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSAACYL
                     DISHPEIEEFLEVRKPSGDFNRKALNLHHGVLITDEFMEAVRDGAEFNLRSPKDQSVR
                     GTVDARGLFQKLVETRLATGEPYIIFIDQVNRMMPKHHRDLGLKVTTSNLCSEITLPT
                     GRDHLGNDRTAVCCLSSLNLETWDEWNGDKRFIEDVMRMLDNVLQDYIDRAPPEMARA
                     KYSASRERSVGLGVMGFHSFLQARGLPFEGAMAKSSNLRVFKHIRAQVDQASMLLANE
                     RGPCPDAADQGVMERFSCKMAIAPTASISIICGGTSACIEPIPANIYTHKTLSGSFSI
                     RNPHLEALLVDKAKNSDAVWNSILEKGGSVQHLDFLTQDEKDCYKTSFEIDQRWLLEL
                     AADRTPYIDQAASLNLFIPADVEKWDLLMLHYRAWELGIKSLYYLRSKSVQRAGFAGG
                     VEADNTPEAAKFELAGSTDYDECLACQ"
     gene            5658..5849
                     /locus_tag="VN034_12605"
     CDS             5658..5849
                     /locus_tag="VN034_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61495.1"
                     /translation="MNVNVTYIALAAAFFALSAVMTAHARKAAEEPGAKKSQLSGILF
                     MIAGTGFVLAGVMPMLRSL"
     gene            5878..6942
                     /locus_tag="VN034_12610"
     CDS             5878..6942
                     /locus_tag="VN034_12610"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HWV61496.1"
                     /translation="MPLLEARKTYKPFEYPWAFDFWKRQQQIHWVPEEVPLGEDCRDW
                     AQKISDHERNLLTQIFRFFTQADIEVQDCYHEKYGRVFKPTEIKMMLTAFSNMETVHI
                     AAYSHLLDTIGMPESEYGMFLEYDEMRAKHDYMGTFGVETDEDIARTLAMFGGFTEGL
                     QLFASFAMLMNFPRFNKMKGMGQIVSWSIRDESLHCEGIIKLFHTFVKERGCLTKAVK
                     EDIIDTCQKTVRLEDAFIDLAFEQGPVPGMTPKEIKRYIRYIADWRLGQLGFQPIYMI
                     DEHPLPWLAPLLNGVEHANFFETRATEYSKGATRGDWNTVWSSFDNRKKAKANDDGAA
                     PGEAGDDGEDMFAKAGIAAE"
     gene            7061..7291
                     /locus_tag="VN034_12615"
     CDS             7061..7291
                     /locus_tag="VN034_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61497.1"
                     /translation="MTEKKKEKLLILSQPQRATLEGLSNRRPQLPTDPIRDELVTLGL
                     VVKQGAKWALTPTGKKVLAASSNRRNSGGFSV"
     gene            7387..8193
                     /locus_tag="VN034_12620"
     CDS             7387..8193
                     /locus_tag="VN034_12620"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HWV61498.1"
                     /translation="MPEDGKVETGSASSTARAEPSLFPARITTRYPANPIGKDYAFLC
                     EAGSEGTFHCKRDTFNKPVRGNEWFSQSLTQHLGIPVPEFRIVEDTNGETYFGSREII
                     STAHQIDVVRFLNYEQLDEIGRAAEWLGRRLSGLTAVDLFLSNPDRTMQNFVFVSKGA
                     TRQLCPIDFADVDLKDITSERFPIVGTNTMRHGRFLRSKHGFVVESALEMIDRIEAIP
                     HGLIDGILRGMPDDWMASDQRNNIVDAWSGKKIAARLSALRAGISDESLL"
     gene            8177..>8492
                     /locus_tag="VN034_12625"
     CDS             8177..>8492
                     /locus_tag="VN034_12625"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HWV61499.1"
                     /translation="MNHSSKFSVIQFVAHPIRNERINVGIVIFSDDGIDVRISKRLER
                     VRSLSAAIDIDQIRQSIIGLREIDAHARSTGCFSENERLESFSSFSSFEFSPMGAFDA
                     RSS"
BASE COUNT         1718 a         2737 c         2501 g         1536 t
ORIGIN      
        1 cgcgcgcatc gccccgatct acgaaggcac caacggcatc caggccgccg atctcgttgg
       61 ccgcaagctc ggcatggcgg gcggcgacct cgtccgcggc ctgatcgacg atatcgcggc
      121 cggcaccgcc gattcccccg aattgcagca gctcgtcgac gcgtgccgcg cggtcaccga
      181 ctggatggtc ggcgcgaaca ccgccgaccg gctcgcgggc agctacccct atctcaccat
      241 gctcgcaacg gcgacttgcg gctggctgat ggcgatccag cacaaggcgg cgcgcaccgc
      301 gctcgacgaa gggcacggcg accgcgtctt tctcgaagcc aagatctcct cgacgcgctt
      361 ctacctgcaa cagatcgttc ccgccgccac agggctggcg gcatcggcgc ttgccggcga
      421 cgccgcgctc gaaccgctgc cagccatggc ctgatatacg cccgtcgccc ccgcgaaggc
      481 gggggccgct gccggcctgc acgacatctc cagcggccgc gttcagaggc gcgtgactct
      541 gaaacgccct ccgcgggggc gacggttgtt tcttcccggc aaaccgccgg atttcgttca
      601 taattaacgc tatgataacc atctaacatt agacgcgacc ggcaattgct ggagacgtgc
      661 gtgcccattc cggtcaaaag tctgatcatc agctggcgaa cgatcgcgct cgcgctgatc
      721 ctcagcgtga tcgtcggcat cagcggcgcg ggcgagctac ccgacaggat gatttcgatg
      781 gcgaccagcc gcgtcgccgc gcggccggta tcgggtgaca ccgttatcgt cgcgctcgac
      841 gacaagacgt tgcaggcaat cggcgagcgt gatttcgacg tcaaccacca tacgcagctt
      901 gtccgcgcga tcgacgcatc gccggcgaag cgactgttca tcgatttttc ctatgaacgc
      961 cgtctcaagg accgcgactt tcccgagctg gcaaaagccg tccgggatat gggcgaccgg
     1021 gtcgtgcttg cggtgccggg aagcagcatg acgggcacca ctgcggtggt cgattattgg
     1081 ccggatcccg ctttcggcac gcgcgcgcag caagcctgca tctgttggga gtatgaattc
     1141 tggcaggtgt ggaaggtgcc gctcgcggtt tcggcgaacg ggcgtttgtt gcccagcttc
     1201 tcggccaaac tcgccgaccg gacgcccgac cggccgaaaa tttttgccct cgacctctct
     1261 tacgatcccg acacgatcac gcaatatagc gcgattgacg tgatgacggg aaagctcgac
     1321 ccccgactgc tgcgcgggaa agatgtgatc ttcgccgcca ccgccgccgc atcgtccgac
     1381 aaacattatc tgcccggcca cgacaagctg cccggcgcct atattcacct gatcgcgggc
     1441 gaggcgctca agcgcggaaa tccggtcgac atcggctggc ttcccgcgct ctcgttcgcc
     1501 tttgtgacgc tgcttggcgc gctcgcatgg cgatccggcc gttggtacgc gacaaccgcg
     1561 cttctcctga cgctcgcgct gcttgtggcc aaggtggttt ggagcctgtc gctcgtaacg
     1621 attcaggttg gccccgcctg ttttctgatc gcggcaatga ccgccaatgt atcgcgcaac
     1681 cggcgtcgtt cgctcgcgca gcgcgaaaac ccggtgtcgg gcttgcccaa tttcgaagcc
     1741 ctgcgctcgc aagcggcgtt cggtagcgcg accgtcatcg cggccaaggt cgtcaatttc
     1801 gaggatctcg ccgccttcat cccgggcgac ggtatcggca agctcgtcga acaggtcacc
     1861 cgccggcttc agctcgcgtc gcagggcacc acgctgcacc acgatctcga cggcagcttc
     1921 gcctggctgg tgccctatta tcagcacagc cagatcgagg gacagcttgc cggcctcgcc
     1981 gcgctgttca atgcgccgct gacgatcggc gagctgcgcg tcgacgtcgc gatcgccttc
     2041 ggggtcaacg acgagttcga gggctcgaac gcgcagcgcc tcgccgccgc gctcgtcgcc
     2101 gccgaacgcg cgatccgtac gcggtcgctc tggaccaaat atacctcgcg ccaaaaggaa
     2161 gacgcgggct ggcaattgag cttccactcg cagctcgaag acgcgctcac cggcggcgac
     2221 atctgggtcg ccttccagcc gcaatatgcc atcgcgacga aaaagctcgt cggggtcgaa
     2281 gcgctggcgc gctggacgca cccgacccgc ggccccatcc cgcccgacga attcatcgtc
     2341 caggccgaaa agagccagga catataccgc ctcacgctgt tcgtgatgga tcaggcgatc
     2401 cggtcggcgg cgcagcttcg cgagcgcggc atccatatca acatgtcggt caacctgtcg
     2461 gcgagcctgc tcgatcacag cgacctcgtc ggcacgatcc gcgtgatgct caccgcgcat
     2521 catctgccgc ccgagatttt gacgatcgag atcaccgaaa ccgcgcagat cgaaaacagc
     2581 cgccaggcgc gccacacgct cgcgcagctg cgccgcacgg gcattcgcct gtcgatcgac
     2641 gactatggca ccggccagtc gaaccttgaa tatctgaccg agatcgaggc cgacgaaatc
     2701 aagatcgaca agcgattcgt gatgacgatg cgcgattcgc agcggaacct cgaagtggtc
     2761 aaatcgacga tcgacctcgc gcaccgcctc ggcgcggtcg cggtcgccga aggcatcgag
     2821 gatggcgaaa ccatggcgct gctcgccggc ctcggctgcg acgtcggtca gggctatcat
     2881 cttgggaaac cgcaattgtt ttccgaactg accgcgactc tcaccgcgct tcctcataac
     2941 cgcatcgcat aactgccaga ttggcggtaa tgcttatagt taagactcga tttaccttac
     3001 taattaaggt atatgctccg ttaactgttc ctcgtgggca gtctgaacag gagattggtc
     3061 atgggttggt tttggcgttg gatgggtggc ggcggctccg gtaacggtgg ctcgcaggcc
     3121 tgacctgcaa acatagagtt agagatcggg cctcggcatc gccggggccc tttctttttg
     3181 cccggcgcag atccgcgcgg tcgtcaggag gacagcgtcc ccgccgtcgc cgcgtcgcgc
     3241 ttcgagtgag ggcgccgcgt cgcgacaagc ccttcttcgc gaccgaaaaa attttgctcc
     3301 ggcacccgca tccacgcctt tccgcgcaaa gccgcggaaa cgctggcgcg acgggttttg
     3361 ttcgtgcatc gggggcgacg agacgaatca aattatcgac gagttgagtc ttcaacctgt
     3421 catttaccgt cttgcatcgc cgcgccgacg tggcactata ggtggtgggt cagccaaggg
     3481 ggcaccccaa taaattgtat caggctgcaa gtggcggatt tccgtcgtcc atcgcatatg
     3541 gacccggaaa aacccttgct gcgcccaaaa aaagtgcccc gtggacgggt ttagatgcta
     3601 ggatcggggc ttcgccctga gtcgaacaag acaggaacaa agcgcactca tgtgcgcgct
     3661 gagatcgggg gcgaatgcga tggattttcg ggaaagcgaa cgggtggaga cgagcgacgt
     3721 ggcgacgatg gaactggcga agaatgacgc tccggcggcg cctgaaacca agggcgaatc
     3781 ggctcccgat tcggcgcccg cggccaagct caacgatggc agcgagccca aggtccacgc
     3841 gcgccgcttc gccatcgtga ccgatgcgag ccgcgacgcg ctgctcaccg atttcggcaa
     3901 ggaaacgctg caggaccgct atctcctccc cggcgaatcg taccaggatc tcttcgcgcg
     3961 cgtcgccgac gcctatgccg acgatcagga tcacgcgcag cgcctctacg actatatctc
     4021 gaagctgtgg ttcatgcccg cgacccccgt gctctcgaac ggcggcaccg gccgcggtct
     4081 gccgatcagc tgctatctga actcggtcga cgacagcctc gagggcatcg tcggcacctg
     4141 gaacgagaat gtctggctcg cttcgcgcgg cggcggcatc ggcacctatt ggggcgcggt
     4201 gcgcggcatc ggcgaacccg tcggcctcaa cggcaagacc agcggcatca tccccttcgt
     4261 ccgcgtgatg gacagcctca ccctcgcgat ttcgcagggc tcgctccgcc gcggctcggc
     4321 cgcctgctac ctcgacatct cgcaccccga gatcgaggag ttcctcgaag tccgcaaacc
     4381 ctcgggcgac ttcaaccgca aggcgctcaa cctgcaccac ggcgtcctca tcaccgacga
     4441 gttcatggaa gctgtccgcg acggcgccga attcaacctc cgctcgccga aggaccagag
     4501 cgtccgcggc accgtcgacg cgcgcgggct gttccagaag ctcgtcgaaa cgcgcctcgc
     4561 aacgggcgag ccctacatca tcttcatcga ccaggtgaac cgcatgatgc ccaagcatca
     4621 tcgcgacctc gggctcaagg tcacgacctc gaacctctgc tccgaaatca cgctgccgac
     4681 gggccgcgac catctcggca acgatcgcac cgcggtctgc tgcctctcgt ccttgaacct
     4741 cgaaacctgg gacgagtgga acggcgacaa gcgcttcatc gaggatgtga tgcgcatgct
     4801 cgacaatgtg cttcaggact atatcgaccg cgccccgccc gaaatggcgc gcgccaaata
     4861 tagcgcgagc cgcgagcgct cggtcggcct cggcgtcatg ggcttccaca gcttcctgca
     4921 ggcgcgcggc ctgccgttcg agggcgcgat ggcgaaatcg tcgaacctcc gcgtcttcaa
     4981 gcatatccgc gcgcaagtcg atcaggcgtc gatgctgctc gcgaacgagc gcggcccctg
     5041 ccccgacgcc gccgaccagg gcgtgatgga gcgtttcagc tgcaagatgg cgatcgcgcc
     5101 gaccgcgtcg atcagcatca tctgcggcgg caccagcgcg tgcatcgagc cgatcccggc
     5161 gaacatctac acgcacaaga cgctctcggg cagcttctcg atccgcaatc cgcatttgga
     5221 agccctcctc gtcgacaagg cgaagaacag cgacgcggtg tggaactcga tcctcgaaaa
     5281 gggcggcagc gtccagcatc tcgacttcct gacgcaggac gaaaaggact gctacaagac
     5341 cagcttcgag atcgaccagc gctggctgct cgaactcgcc gccgaccgca cgccctatat
     5401 cgatcaggcc gcgtcgctga acctcttcat ccccgccgac gtcgagaaat gggacctgct
     5461 catgctccac tatcgcgcgt gggaactcgg catcaaatcg ctctattatc tccgctcgaa
     5521 atcggtccag cgcgccggct tcgcgggcgg ggtcgaggcc gacaacacgc ccgaggccgc
     5581 gaagttcgaa ctcgccggca gcaccgacta tgacgagtgt ctggcctgtc agtgatggac
     5641 cgcgcggaga tcacgcgatg aatgtgaacg tcacatacat cgcgctcgcc gcggccttct
     5701 tcgcgctcag cgccgtcatg acggcgcacg cgcgcaaggc ggccgaagag cccggagcga
     5761 aaaagagcca actctcgggc atcctgttca tgattgccgg tacggggttc gtcctcgccg
     5821 gcgtgatgcc catgctacgg tccctgtgac cgaccgaatt tctggagtgt aaccgccatg
     5881 cctcttctcg aagcccgcaa gacctacaag cccttcgaat atccgtgggc gttcgatttc
     5941 tggaagcgcc agcagcagat ccactgggtg cccgaggaag tgccgctggg cgaggattgc
     6001 cgcgactggg cgcaaaagat ttcggaccac gaacgcaacc tgctgacgca gattttccgc
     6061 ttcttcacgc aggccgatat cgaggtgcag gattgctacc acgaaaaata tggccgcgtg
     6121 ttcaagccga ccgaaatcaa gatgatgctg accgcgttca gcaacatgga aacggtgcat
     6181 atcgcggcgt acagccattt gctcgacacg atcggcatgc ccgaaagcga atatggcatg
     6241 ttcctcgaat atgacgagat gcgcgccaag cacgactata tgggcacctt cggcgtcgaa
     6301 accgacgaag acatcgcgcg cacgctcgcg atgttcggcg gctttaccga aggcttgcag
     6361 ctcttcgcca gcttcgcgat gctgatgaac ttcccgcgct tcaacaagat gaagggcatg
     6421 ggccagatcg tcagctggtc gatccgcgac gaaagcctcc actgcgaggg catcatcaag
     6481 ctgttccaca ccttcgtgaa ggaacgcggc tgcctgacca aggcggtgaa ggaagacatc
     6541 atcgacacct gccagaagac ggtgcggctc gaggatgcgt tcatcgacct cgccttcgaa
     6601 cagggccccg tccccggcat gacgccgaag gagatcaagc gctacatccg ctacatcgcc
     6661 gactggcgtt tggggcaact cggtttccag ccgatctaca tgatcgacga acacccgctc
     6721 ccctggctcg ccccgctctt gaacggcgtc gagcacgcca acttcttcga aacgcgcgcg
     6781 accgaatatt cgaagggcgc gacgcgcggc gactggaaca ccgtctggtc gagcttcgac
     6841 aaccgcaaga aggcgaaagc caacgacgac ggcgccgcgc ccggcgaagc gggcgacgac
     6901 ggcgaagata tgttcgcgaa agccggcatc gccgcggagt gacataaatc ccctcccgca
     6961 agcgggaggg gcagcgagac ttgcgggcag agcccgctag tcacagcggg gtgggcacca
     7021 acgcccaccc aacgcatcaa acaagaagcc cgaggccaaa atgaccgaaa agaaaaaaga
     7081 aaaactcctg atcctcagcc agccgcagcg cgcgaccctc gaaggcctct cgaaccgccg
     7141 cccccaactc cccaccgacc cgatccgcga cgaactcgtc acgctgggcc tcgtcgtcaa
     7201 gcagggcgcg aagtgggcgc tgaccccgac gggcaagaag gtgctcgcgg ccagctcgaa
     7261 ccggcggaac tcgggcgggt tttcggttta gggcgggaca tgtaaaaaca tctcaatgct
     7321 ggcctaggga gggggccgct gcaacaagaa gcgagtagcg tcgtgacaca aggatggcac
     7381 atccagatgc ccgaagatgg gaaagtggag accggcagcg cctcatcaac ggctcgtgcg
     7441 gaaccgagct tatttcctgc acgcataaca acgcgctatc ctgccaatcc cattgggaag
     7501 gactatgcct ttctctgcga ggcgggaagc gaaggcacct tccattgcaa acgtgatacc
     7561 ttcaacaagc ctgtccgcgg caatgaatgg ttttctcagt cccttacgca acatctcggc
     7621 atcccggtgc ctgagtttcg catcgtcgaa gacacgaatg gcgaaactta cttcggttct
     7681 cgcgaaatca tttcgacggc gcatcagatc gacgtggttc gtttcctaaa ctatgagcag
     7741 ttggacgaaa tcgggcgagc cgccgaatgg ctcgggagaa ggctgtcggg attaacggct
     7801 gtcgacctct ttctcagcaa tcccgaccgt acaatgcaga atttcgtctt tgtctcgaaa
     7861 ggcgccactc ggcagctttg ccccattgat tttgcggacg ttgatctgaa agatatcacc
     7921 agtgagcggt ttccgattgt cggaacgaac acgatgcggc atggtagatt tctacgaagc
     7981 aaacacggat ttgtcgtgga atcggctttg gaaatgatcg atagaattga agctattccg
     8041 catggtctaa tcgatggcat tttgcggggt atgccggatg actggatggc tagtgatcaa
     8101 cggaacaaca tcgtcgacgc ttggtcaggc aaaaaaatcg cagctcggct gtccgcgttg
     8161 cgggctggca taagcgatga atcactcctc taaattctcc gttattcagt ttgtcgccca
     8221 ccccatccgc aacgaacgca taaatgtcgg aatcgtcatc tttagcgatg acggtataga
     8281 cgttcgcatt tcgaagcggt tggaacgtgt acgatcgttg tcggcagcca tcgacattga
     8341 tcagattcgc caatccataa taggattgcg agaaattgac gcacatgctc gatcgaccgg
     8401 ctgcttttcc gaaaacgaac gcctcgaaag tttttcgtcc ttctcgagtt tcgaattttc
     8461 ccccatggga gccttcgatg cacgctcttc tt
//
LOCUS       DATMSU010000475         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_140572,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000475 DATMSU010000000
VERSION     DATMSU010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140572"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VN034_12630"
     CDS             complement(<1..561)
                     /locus_tag="VN034_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61500.1"
                     /translation="MTTTDRETAPTMRRRDNWRFVAAILVALAAALAVYILAEAGAGG
                     AIGYALMVVLPAALSGFIAWAGSIGRNWSRAAFLSVPLWLALAATAIGAIFLSEGIVC
                     LVMVLPFWVGFGYLGIWPVYLYRRSRQVVDPATFRAHALLLLPFLALLVDQQVAPPRE
                     TRMVIREIIVDAPAAAVWPRLLAIPAI"
     gene            651..1616
                     /gene="trxB"
                     /locus_tag="VN034_12635"
     CDS             651..1616
                     /gene="trxB"
                     /locus_tag="VN034_12635"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWV61501.1"
                     /translation="MPAVHHTKMLILGSGPAGLSAAIYAARAGMMPIVVQGLQPGGQL
                     TITTDVENYPGFAEVIQGPWLMEQMTAQATHVGTSMIWDTIVDVDLSRRPFKLTGDGG
                     DVYMAETLVIATGAQAKWLGVPGEQELGGKGVSACATCDGFFYRGKKVVVIGGGNTAV
                     EEALYLTNHSDDVTLIHRRDHLRAEKILQDRLFANPKIKVLWNKKVDRFVAGEGVSGL
                     VGVDLIDTVTGAASHEPTDGGFVAIGHSPSTELFKDKLPLDADGYLEVTPGTSLTSIP
                     GVFAAGDVTDKIYRQAVTAAGMGCMAALDAERFLAEAEYHAMATA"
     gene            complement(1703..>2233)
                     /locus_tag="VN034_12640"
     CDS             complement(1703..>2233)
                     /locus_tag="VN034_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase 1, exosortase A system-associated"
                     /protein_id="HWV61502.1"
                     /translation="VTRVVAFGNCDAASALLLHQPLALGALILANPWTYEGPGEDGGD
                     EPALPPASAIRARYLSRLRDPKSLLRLLRGEIDFRKLVRGLSALKQAPRPAAADSLAH
                     SLESALAELSCPVTILLATGDRTAQAFAESCGPALAGSGKEPGAIRVERLASASHSFA
                     GEDGDWLAARVLAALE"
BASE COUNT          402 a          767 c          746 g          318 t
ORIGIN      
        1 gatcgccggg atcgcgagca ggcgcggcca caccgccgcg gcgggcgcgt cgacgatgat
       61 ctcgcggatc accatccgcg tctcgcgcgg cggggcgacc tgctgatcga ccagcagcgc
      121 aaggaagggc aacagcagca gcgcatgggc gcgaaaggtc gcgggatcga cgacctgccg
      181 gctgcggcga tacagataga cgggccagat gccgaggtag ccgaagccca cccagaaagg
      241 cagcaccatg acgaggcaca cgatgccttc gctcaggaaa atcgccccga tcgcggtcgc
      301 ggcgagcgcc agccacagcg gcaccgacag aaaggccgcg cgcgaccagt tgcgcccgat
      361 gcttccggcc caggcgatga agcccgacag cgcggcaggc agcacgacca tcagcgcata
      421 gccgatcgcg ccgcccgctc ccgcttccgc caatatatat acggcaagcg ccgcggcgag
      481 cgcgacgagg atcgcggcaa caaatctcca attgtcgcgg cgccgcatcg tcggcgccgt
      541 ttcgcgatcg gtcgttgtca cgcgggctcc ctcttggcgc gcagcctgtc ccttgctagc
      601 gcacttggca acacccatct gtgcctgccg cacacccaag aaagatcatc atgcccgccg
      661 ttcaccatac caagatgctc atcctcggtt ccggccccgc cggcctctcg gccgccatct
      721 atgccgcgcg tgcgggcatg atgccgatcg tggtgcaggg gctccagccc ggcggccagc
      781 tgacgatcac caccgacgtc gaaaactatc ccggtttcgc cgaggtgatc cagggcccgt
      841 ggctgatgga acagatgacc gcgcaggcga cgcatgtcgg caccagcatg atctgggaca
      901 cgatcgtcga cgtcgacctg tcacgccgcc cgttcaagct gaccggcgac ggcggcgacg
      961 tctatatggc cgaaacgctg gtgatcgcga ccggcgcgca ggccaaatgg ctcggcgtcc
     1021 cgggcgagca ggaactgggc ggcaagggcg tctcggcctg cgcgacgtgc gacggcttct
     1081 tctatcgcgg caagaaggtg gttgtgatcg gcggcggcaa taccgcggtc gaggaggcgc
     1141 tttacctcac caaccatagc gacgacgtca cgctgatcca ccgccgcgat catctgcgcg
     1201 ccgaaaagat tttgcaggac cgcctgttcg cgaatccgaa gatcaaggtt ctgtggaaca
     1261 agaaggtcga tcgcttcgtc gcgggtgagg gcgtgtcggg gctcgtgggc gtcgacctga
     1321 tcgacaccgt cactggcgcg gcgagccacg agccgaccga cgggggtttc gtcgcgatcg
     1381 gccacagccc gtcgaccgag ctcttcaaag acaagctgcc gctcgacgcc gacggctatc
     1441 tggaagtgac gccgggcacg tcgctgactt cgattccggg cgtcttcgcg gcgggcgacg
     1501 tcaccgacaa aatctatcgc caggcggtaa ccgccgcggg catgggctgt atggccgcgc
     1561 tcgacgccga acgcttcctc gccgaagccg aatatcacgc gatggcgacg gcctgacaac
     1621 attcgtcgcc cccgtgaagg cgggggccgc tggaggcatg gcgcaaggcc aaggacggcc
     1681 cccgccttcg cgggggcgac ggctattcca aggccgccaa aactcgcgcc gccagccaat
     1741 ctccatcctc gccggcaaag ctgtgcgacg cgctggcgag gcgctcgacg cggatcgcgc
     1801 ccggctcttt cccgctaccg gccagcgccg gaccgcaact ctccgcaaag gcctgcgcgg
     1861 tgcggtcgcc ggtcgcgagc aggatcgtca ccggacaaga aagttcggcg agcgcgcttt
     1921 cgaggctgtg ggcgaggctg tcggccgccg ccggtcgcgg cgcctgtttg agcgccgaaa
     1981 ggccgcggac caatttgcgg aaatcgatct cgcccctcag caggcggagc aggctcttcg
     2041 gatcgcgcaa tcgcgacagg tagcgcgcgc gaatggcgga cgcggggggc agcgcgggtt
     2101 cgtcgccgcc atcctcaccg ggcccttcgt aagtccacgg attggcgagg atcagcgcgc
     2161 ccagcgccag cggctggtgc agcagcagcg cgctcgccgc gtcgcaattg ccaaaggcga
     2221 cgactcgcgt gac
//
LOCUS       DATMSU010000476         6623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_159807,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000476 DATMSU010000000
VERSION     DATMSU010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159807"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /gene="folD"
                     /locus_tag="VN034_12645"
     CDS             <1..791
                     /gene="folD"
                     /locus_tag="VN034_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HWV61503.1"
                     /translation="LAVVLVGDDPASAVYVGSKGKATVAAGMESFEHRLPATARQEEV
                     EALLAKLNADETVDGILLQLPLPGHLDEQAAVATIDPDKDVDGLTPVSAGRLALGIPG
                     MVPCTPYGCLLLLQDRLGDLSGKDAIVVGRSILVGKPMGQLLLGANCTVTMAHSRTKD
                     LPDLVRRADIVVAAVGRSEMVKGDWIRPGATVIDVGINRLPPAGGETKGRLVGDVDYA
                     EAASVADAITPVPGGVGPMTIACLLRNTLVAAHRRAGLADPEGF"
     gene            791..1462
                     /locus_tag="VN034_12650"
     CDS             791..1462
                     /locus_tag="VN034_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61504.1"
                     /translation="MRQLLVPALALSLLAGCASDPDDIRNRPLAANPSAFIAAEIRFA
                     QLAQEEGQWTAFRETSHPDAVMFVPERVKAQDWLKAQKDPAEAVKWQPHAVYVSCDGN
                     SGVTTGAWQKGPANGFFTTVWARDKKGRLSWVLDHGDALEAPREAPEFIASKRAVCGS
                     RPAVPIEAGGAGEDRVVGLSGDQTLSWTSTIRADKSRRVTIRLWDGKEMATVIDDQVA
                     APKQP"
     gene            1459..2088
                     /locus_tag="VN034_12655"
     CDS             1459..2088
                     /locus_tag="VN034_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWV61505.1"
                     /translation="MIDLFVSAFVTLFVVIDPPGCAPIYASLTTGASAQQRRSMAIRA
                     TIIAGLILVFFAMFGEALLSFLHIDLDSFRIAGGIMLFIIAIDMVFEKRTERREQRAE
                     KVKATPEVEDVSVFPMAMPMLAGPGSIASVMLLVSQNNGLERAFIIFGALLLVLLLTL
                     AALLAAGPLMRLIGNKGEAVITRLLGVLLAALAAQFVIDGLKASFPSLA"
     gene            complement(2142..2831)
                     /locus_tag="VN034_12660"
     CDS             complement(2142..2831)
                     /locus_tag="VN034_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWV61506.1"
                     /translation="MNSRTIKLTILTSIGAIALTGCVTDPVTGERKISKAAIGGVGGA
                     LGGYLLGDLIGGKNSRTEEIVGAGIGAVAGAGVGYYMDQQEKKLRERTAGTGIDVERQ
                     GDQLVLNMPGDVTFDLNSAMVKSQFRSALDNVASTLAEYPSTYIDVYGHTDSTGSDSY
                     NQGLSERRAASVADYLGGRGIQRARMATLGYGESQLKCSPERGEADYQCNRRVEIRIA
                     PVTQNDVNAAR"
     gene            3051..4373
                     /locus_tag="VN034_12665"
     CDS             3051..4373
                     /locus_tag="VN034_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWV61507.1"
                     /translation="MTPFPWSDVAIIAVLVLLNGVFAMSELAIVSSRDPRLQAAEKRG
                     SRGAKIARQLASDPGRFLSTVQVGITLIGVLTGAYSGASLGQPVAERLQAAFGLEPET
                     ALSTAFALVIALTTYASLIAGELVPKQFALRAPERIAIIVAPMMYWLSRIAAPLVWIL
                     DNSSALVFRILGLNRESEDRVTAEELHLIVAEASKSGVIEESERAIISGVVRLADRPV
                     REVMTPRKDVDWVDISLDARGVRDKLLETPHSRLPVARGSVDDIVGVVQARDIAAALF
                     KGETLDLEQLMRPARVIHDQIDAMDALEALRVAEVPMLLVHDEYGHFDGLVTPADLLS
                     AIAGEFASDQDIGSEPFVVERDDGSLLIAGSMPADQMAERLGIELGEDRDYATAAGHV
                     LAILKHLPTEGEKFTDRGWRFEIIDMDGRKIDKLLVTEVRKPKDAEDA"
     gene            complement(4646..5254)
                     /locus_tag="VN034_12670"
     CDS             complement(4646..5254)
                     /locus_tag="VN034_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWV61508.1"
                     /translation="MTDFILNLIQSWGYAGIFILMFLENVFPPIPSEVIMGLGGIAVA
                     QGRFDFWTLVAVAVAGTTAGNWIWYAIGRWIGYERLKPFIDRHGRWLTLDWDEVERLH
                     DLFLKYGPAIVFIARFMPVARTMVSLPAGMVQMNQLKFLIWTAAGSTIWIAALAGAGS
                     WFGKQFANLDAFVGPLALAAIGSLIALYIYRVITWKPKTGGK"
     gene            complement(5278..6228)
                     /gene="gshB"
                     /locus_tag="VN034_12675"
     CDS             complement(5278..6228)
                     /gene="gshB"
                     /locus_tag="VN034_12675"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HWV61509.1"
                     /translation="MTLRAAVQMDPMDGINIGGDSSFHLILKALERGYELYHYDVRGL
                     TWEAGRLTTKAHRILEAQREAGAHYKFGDEVLLDLGRDLDAVLMRQDPPFDLGYLTGT
                     WLLERIGHETLVVNDPVSVRNAPEKVFVLDFPEFMPPTMVTRDLDAVKDFQARYGAVV
                     IKPLHGNGGKAVFKIDADGGNLGALVELFGQVWPEPFMVQQFLPSVSQGDKRIVLVDG
                     EVAGAINRKPGEGEFRSNLAVGGYAEAAGLTPREQEICDVLGPALRERGLVFVGIDVI
                     GGEWLTEINVTSPTGIVAIDRFNDSDTAGMIWDAIKRRIG"
     gene            complement(6399..>6623)
                     /locus_tag="VN034_12680"
     CDS             complement(6399..>6623)
                     /locus_tag="VN034_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61510.1"
                     /translation="VARRGRMIAFVEVKWRDRPEDLDLAIDPYRLRRVAAAAEMLAPR
                     FAGPRDDIRIDVMLLAPRRLPRHLVHVWQP"
BASE COUNT         1071 a         2162 c         2238 g         1152 t
ORIGIN      
        1 ggctcgccgt ggttctcgtc ggcgacgatc cggcgagcgc ggtctacgtc gggtcgaagg
       61 gcaaggcgac ggtcgccgcg ggcatggaaa gcttcgagca ccgcctgccc gcgactgccc
      121 gccaggaaga ggtcgaggcg ctgctggcaa agctcaacgc cgacgagacg gtcgatggca
      181 tcctgctgca attgccgctg cccggccatc tcgacgagca ggcggcggtc gcgacgatcg
      241 atcccgacaa ggatgtcgac gggctgaccc cggtcagcgc cgggcgcctc gcgctcggca
      301 tcccgggcat ggtgccgtgc acaccctatg gctgcctgct gctgctgcag gaccggctcg
      361 gcgacctgtc gggcaaggat gcgatcgtcg tcgggcgctc gatcctcgtc ggcaagccga
      421 tggggcaatt gctgctcggc gccaattgca ccgtgacgat ggcgcacagc cggacgaagg
      481 atctgcccga tctcgtgcgc cgcgccgaca tcgtcgtcgc cgcggtcggc cgatccgaaa
      541 tggtcaaggg cgactggatc aggcccggcg cgaccgtgat cgacgtcggc atcaaccgcc
      601 tgccgcccgc ggggggcgag accaagggac ggctcgtcgg cgacgtcgac tatgccgaag
      661 ccgcaagtgt tgccgatgcg atcacgccgg ttcccggcgg cgtcggcccg atgaccatcg
      721 cgtgcctgct gcgcaacacg ctcgtcgcgg cgcatcgccg cgcggggctt gccgatccgg
      781 agggtttttg atgcgccagc tgctggtccc cgcgcttgcc ctgtcgttgc tcgcggggtg
      841 cgcttcggat cccgacgata ttcgcaaccg gccgctggca gccaacccga gcgcgttcat
      901 cgccgccgag atccgcttcg cgcagctcgc gcaggaggag gggcaatgga cggcgttccg
      961 tgaaacgtcg catcccgacg ccgtcatgtt cgtgcccgag cgggtgaagg cgcaggactg
     1021 gctgaaggcg caaaaggacc cggccgaggc ggtgaagtgg cagccgcacg ccgtctatgt
     1081 cagctgcgac ggcaacagcg gggtcaccac cggcgcctgg caaaaggggc cggcgaacgg
     1141 ctttttcacc accgtctggg cgcgcgacaa gaaggggcgg ttgagctggg tcctcgacca
     1201 tggcgacgcg ctggaggcgc cgcgcgaggc gcccgagttt atcgcatcga aacgcgcggt
     1261 ctgcggttcg cggcccgcgg tgccgatcga agcgggcggc gcgggcgagg acagggtggt
     1321 cggcctgtcc ggcgaccaga cgctgagctg gacctcgacc atccgcgccg acaagtcgcg
     1381 ccgcgtcacg atccgcctgt gggacggcaa ggaaatggcg accgtcatcg acgatcaggt
     1441 cgcggcgcca aagcagccat gatcgacctt ttcgtctcgg ccttcgtcac gctgttcgtc
     1501 gtcatcgacc cgccaggctg cgcgccgatc tatgccagcc tgacgacggg cgcgagcgcg
     1561 cagcagcgcc gttcgatggc gatccgcgcg acgatcatcg cggggctgat cctcgttttc
     1621 ttcgcgatgt tcggcgaagc gctgctgagc ttcctccaca tcgatctcga cagtttccgc
     1681 atcgcgggcg ggatcatgct gttcatcatc gcgatcgaca tggtgttcga aaagcgcacc
     1741 gaacgccgcg aacagcgcgc cgaaaaggtc aaggcgaccc ccgaggtcga ggatgtgtcg
     1801 gtctttccga tggcgatgcc gatgctcgcc gggccggggt cgatcgcgtc ggtgatgctg
     1861 ctggtgtcgc agaacaatgg tctcgagcgc gccttcatca tcttcggcgc gctgcttctg
     1921 gtgctgctgc tcacgctggc ggcgctgctc gccgcgggtc cgctgatgcg gctgatcggc
     1981 aacaagggcg aagcggtgat cacgcggctg ctcggcgtgt tgctcgcggc gctcgcggcg
     2041 cagttcgtga tcgacgggct gaaggctagc ttcccgagcc tcgcataaaa ggggcgccgc
     2101 ctttcggcga cgccctttct attccacggg aatggaacct cttaccgcgc cgcgttgacg
     2161 tcgttctggg tgaccggcgc gatgcggatc tcgacgcggc ggttgcactg ataatcggcc
     2221 tcgccgcgtt cgggcgagca tttcagctgc gattcgccgt agccgagcgt cgccatgcgc
     2281 gcacgctgga tgccgcggcc gcccagataa tcggcgaccg acgcggcgcg gcgttcggac
     2341 aggccctgat tatagctgtc gctgcccgtc gaatcggtgt ggccatagac gtcgatatag
     2401 gtgctcggat attcggcgag cgtcgaggcg acattgtcga gcgcgctgcg gaactgcgac
     2461 ttcaccatcg cgctgttgag gtcgaaggtc acgtcgccgg gcatgttgag cacgagctgg
     2521 tcgccctggc gctcgacgtc gatgccggtg ccggcggtgc gttcgcgcag cttcttttcc
     2581 tgttggtcca tataatagcc gacgcccgcg cccgcgaccg cgcctatccc tgccccgacg
     2641 atttcctcgg tgcggctgtt cttgccgccg atcaggtcgc cgagcagata accgccgagc
     2701 gcgccgccga cgccgccgat cgcggccttc gatatcttgc gttcgccggt caccggatcg
     2761 gtgacgcaac ccgtcagcgc gatcgcgccg atgctggtga gaatggtgag cttgatcgtc
     2821 ctgctgttca tttttcgctc ccttatccgg cggatgcctc tggtcattcg catgccgcgc
     2881 ctgtgtgccg ataacatatg gccggcgatc tggttcccga atagcgctga acggcggctg
     2941 agcagagcat tcaggtgagc accattgtgc aacgctgcgc ctttcgctta tagggtggac
     3001 atgaccgacg atgacagggg ccgggcgctg acgcgccgca cgaccgccca atgacccctt
     3061 tcccttggtc cgacgtcgcg atcatcgcgg tcctcgtcct cctcaacggc gttttcgcca
     3121 tgtccgaact cgcgatcgtc tcgtcgcgcg acccgcgatt gcaggcggcc gagaagcgcg
     3181 gcagccgcgg cgcgaaaatc gcgcgccagc tcgcctccga tcccgggcgc ttcctgtcga
     3241 cggtgcaggt tggcatcacg ctgatcggcg tgctgaccgg cgcctattcg ggtgcgagcc
     3301 tggggcagcc cgtcgccgag cggctgcagg cggcattcgg tctcgagccg gagaccgcct
     3361 tgtcgacggc gttcgcgctc gtcatcgcgc tcaccaccta tgcgtcgctg atcgccggcg
     3421 agctggtgcc caagcagttc gcgctgcgcg cgcccgagcg gatcgcgatc atcgtcgcgc
     3481 cgatgatgta ttggctgtcg cggatcgcgg cgccgctggt gtggattctc gacaatagtt
     3541 cggcgctggt gttccgcatc ctcggcctca accgcgaatc ggaggaccgg gtgaccgccg
     3601 aggaattgca cctgatcgtc gccgaggcgt cgaaatcggg ggtgatcgag gaaagcgaac
     3661 gcgcgatcat ctcgggcgtc gtgcggctcg ccgaccgtcc ggtgcgcgag gtgatgacgc
     3721 cgcgcaagga cgtcgactgg gtcgacatct cgctcgatgc aaggggtgtg cgcgacaaat
     3781 tgctcgaaac cccgcacagc cgcctgccgg tcgcgcgcgg gtcggtcgac gacatcgtcg
     3841 gggtcgtcca ggcgcgcgac atcgccgcgg cgctgttcaa gggcgagacg ctcgacctcg
     3901 aacaattgat gcgcccggcg cgggtcatcc acgaccagat cgacgcgatg gacgcgctcg
     3961 aggcgctccg cgtcgccgag gtgccgatgc tgctcgtcca cgacgaatat ggccatttcg
     4021 acggtctggt gacccccgcc gacctgcttt cggcgatcgc gggcgaattc gcgtcggatc
     4081 aggacatcgg cagcgagcct ttcgtcgtcg aacgcgacga cggcagcctg ctcatcgcgg
     4141 gatcgatgcc cgccgaccag atggccgagc ggctcggcat cgagctcggc gaggaccgcg
     4201 actatgcgac cgcggcgggc catgtcctcg ctatcctgaa gcatctgccg accgagggcg
     4261 aaaagttcac cgaccggggc tggcgcttcg aaatcatcga catggacggg cgcaagatcg
     4321 acaaattgct cgtcaccgag gtccgcaagc cgaaggatgc cgaggacgcg tagcgcaccg
     4381 cgtccgccca atttacgtcc gaagttgtca aacttgtcgt tcccctcggg taaaatgata
     4441 cccgtcgcgg ctggaccggc cttgtggatg cgccggcggc tgccgtcgaa caggaaggat
     4501 gggaaggcga agcatgccat ccgccagttt gaagcatggc ggaggaatgt aggaagggat
     4561 tattcgcggg gcgtcagccc tggggtggag cgcggacgtg tcctattccg ccatcccggc
     4621 gctcgccgca caacgaccgg ccgaatcatt ttcccccggt cttcggcttc caggtgatca
     4681 cccggtagat atagagcgcg atcagcgagc cgatcgcggc cagcgccaag gggccgacga
     4741 aggcgtcgag gtttgcgaat tgcttgccga accagctgcc cgcgccggcg agcgcggcga
     4801 tccagatcgt cgagccggcg gcggtccaga tcaggaattt gagctggttc atctgcacca
     4861 tgcccgcggg cagcgagacc atcgtccgcg cgacgggcat gaagcgcgcg atgaagacga
     4921 tcgcggggcc atatttcagg aagagatcgt gcaaccgctc gacctcgtcc cagtcgagcg
     4981 tcagccagcg gccgtggcgg tcgatgaagg gtttgagccg ctcatagccg atccagcgcc
     5041 cgatcgcgta ccagatccag ttgcccgcgg tcgttcccgc gaccgcgacc gcgacgagcg
     5101 tccagaaatc gaagcggccc tgcgcaacgg cgatcccgcc caatcccatg atcacttcgg
     5161 acgggatcgg cggaaacaca ttttcgagga acatgaggat gaaaatcccc gcatagcccc
     5221 agctctggat caggttcagg atgaaatcgg tcatggggga gtaacgcctt ccctgtgtca
     5281 gccgatgcgt cgcttgatcg catcccagat catgcccgcc gtatcgctgt cgttgaaacg
     5341 gtcgatcgcg acgatcccgg tcggcgaggt gacgttgatt tcggtgagcc attcgccgcc
     5401 gatcacgtcg atgccgacga agaccagccc gcgctcgcgg agcgcggggc cgaggacgtc
     5461 gcaaatctcc tgctcgcgcg gagtcagtcc ggcagcctcg gcatagccgc cgaccgcgag
     5521 gttcgagcgg aattcgcctt cgccgggctt gcggttgatc gcgcccgcga cttcgccgtc
     5581 gacgagcacg atgcgcttgt cgccctggct gacgctcggc aggaattgct ggaccatgaa
     5641 gggttcgggc cacacctgtc cgaacagctc gacgagcgcg cccagattgc cgccgtcggc
     5701 gtcgatcttg aatactgcct tgccgccatt gccgtggagc ggtttgatca cgacggcgcc
     5761 gtagcgcgcc tgaaaatcct tcaccgcgtc gaggtcgcgg gtcaccattg tcggcggcat
     5821 gaactcggga aagtcgagca cgaagacctt ttcgggcgcg ttgcggaccg agacggggtc
     5881 gttgactacc agcgtctcgt gtccgatgcg ttcgagcagc caagtgccgg tgaggtaacc
     5941 gaggtcgaag ggcgggtcct gccgcatcag cacggcgtcg aggtcgcggc cgaggtcgag
     6001 cagcacctcg tcgccgaact tgtaatgcgc gccggcctcg cgctgcgcct caaggatgcg
     6061 atgcgccttc gtcgtcagcc gcccggcctc ccacgtcagc ccgcgcacgt cgtagtgata
     6121 aagctcgtac ccgcgttcga gcgccttcag gatcaggtgg aaactgctgt cgccgccgat
     6181 gttgatgccg tccatcgggt ccatttgcac ggctgcgcgc agggtcattc gtctatcctt
     6241 ctcaagcccc tccccttcag gggaggggtt ggggtggggc tgtcggcgta gcgcaaggcc
     6301 gatggccccc accccgctcg attaaagtcc cggcttgtgc cgggccaagt ctcgctaccc
     6361 ctcccctgaa ggggaggggt ttgaatattt agacatcctc acggctgcca aacatgaacc
     6421 agatggcgtg gcaggcggcg cggcgcaagg agcatcacgt cgatcctgat gtcgtcgcgc
     6481 gggcccgcga agcgcggggc gagcatttcc gcggccgcgg cgacgcggcg gaggcgatac
     6541 ggatcgatcg cgaggtcgag gtcttcgggc cggtcgcgcc acttgacctc gacgaaggcg
     6601 atcatccgcc cgcgccgcgc gac
//
LOCUS       DATMSU010000477        10239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_102742,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000477 DATMSU010000000
VERSION     DATMSU010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102742"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1329
                     /locus_tag="VN034_12685"
     CDS             61..1329
                     /locus_tag="VN034_12685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWV61511.1"
                     /translation="MTDPLANSLYAATAHPWRARAFEGDLECDVAVIGGGFTGLSAAL
                     ACAERGFSVILIEREHVGFGASGRNGGQLIPGLRWTASELEAEFGRERADALFGLCWR
                     DNRAKARIAKHGIDCDLKAGHLEAAWTPKDFDAMRREADYLANRFDYRTDIVEKNEMA
                     RHIVSPLYHGGIHDPQAGHFHPLDYALGLAKAAEAAGVRIWEGEPVRRIAEFSDGSSE
                     LVTERAIVGARYVIDATDSWIGDLEPDLGRYTVPIMNYNIATAPLANADTLMPTDAAV
                     ADSRFVLNYFRLSADKRLIFGGGERYSQTPPRDIAAFVRPFMAQVFPQIADAKIDYGW
                     GGAVAVTMNRLPHIGRRGNVFFAHGFSGHGALVTTLAGELIAEAMAGTAERFDILANL
                     PSKPFPGGKWLRRPLATLGLLWYALRDRLG"
     gene            1340..2731
                     /locus_tag="VN034_12690"
     CDS             1340..2731
                     /locus_tag="VN034_12690"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWV61512.1"
                     /translation="MSKSSGVIAPRSEAEAFFKANPDVDSIEMIYTDMGGVPRGKRLR
                     QHEVLAVYDSGRMMPGSITVVDITGQDTIETGLVWEDGDADRSMKPIPGTLVRTPWGG
                     DHAAQFLVGFYELDGTPHDLDPRHVLGRVIDRYTADGLTPVLAVELEFYLVDPRRARD
                     GRIRPARPGYSRDTPRNVEVYGLRELDDFRPFFDALYAATDVQGLPLESAISEFAPGQ
                     FELTLRHKPDALRACDDAIMYKRLVKAIAQQQGLEATFMAKPFADQAGSGMHIHVSVD
                     DAAGNNIFASDDPEGTPALRHAIGGMIGSVGDGFALFAPHANSYRRFKANSYAPVAPT
                     WGVNNRTVSFRIPAGPPPSRHVEHRACGADANPYLAVAAVLSGMHHGMANQSDPGPAV
                     VGNGYDRDNSGDDKPPSNWFAAVDRFHASKLMRDYLGERFVDMFSIVKRVEQDNYFSV
                     VPTLDYDWYLRNA"
     gene            2823..3929
                     /locus_tag="VN034_12695"
     CDS             2823..3929
                     /locus_tag="VN034_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWV61513.1"
                     /translation="MDFNDLIKQTRGRRSLLQALGVAAVGISFGGLAACSKQGGKTLA
                     NGEEAKLNFYNWDTYIGENTLDNFKEATGVDVTMDLFDSNDVLFAKFKAGNPGYDVIV
                     PSNDFVERMSKAGMLLPLDHSLIPNKRNIDPAYINVEYDLQRKFSMPYTWLALGIGYR
                     KSKVSATPDSWKVLFDSPEYAGRIAWLSEAGDMFRLYGKYLGKSVNALTPADIATIER
                     MMIKQKPNVKKFHEDDGQDLLLKGDCDVVLEYNGDIAQAMVEDKDIDFVIPKEGSQLN
                     SDNLCIPKGAPHPKNAHAFINYILDANVDKHITETILYPTPNTAARKLMPDSYQNNPV
                     IFPPAEALAKCEYARFDPELQPLYEEAFTRVRAA"
     gene            3953..4858
                     /locus_tag="VN034_12700"
     CDS             3953..4858
                     /locus_tag="VN034_12700"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV61514.1"
                     /translation="MAEQDWKTNRGAFAAVSLPTLFWVILFFLVPMAIVWLYSFGENR
                     GLTEIEISGTLDNYKRATEWLYLTIFGKSFAVAALVTLICLIVGFPVAMAITFASEKW
                     RPWLLLAIMLPFWTNLLIRTYALMMLLGTQGFANKGLGALWDGASWLKALVGLEPLPT
                     WEPVQLLFNNFAVVFGLVYVHLPFMVLPLYAALDRLDRSLIEASLDLGAGHFRTIMRI
                     VVPLAAPGIVAGVMITLIPALGAYLTPDLMGGTDSQMIANVIERQFKKANDWPFGAAL
                     SFLLIYAMFALIAIQSMRRKVPEVH"
     gene            4858..5700
                     /locus_tag="VN034_12705"
     CDS             4858..5700
                     /locus_tag="VN034_12705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWV61515.1"
                     /translation="MALFAHTPRAPLEYSRTLWMRLWVGAVMLFLYAPLVVLVIFSFN
                     DSKRNVVWRGFTTKYYEKALGNDQLVEALLNSLTIAALATIVSLILGAVAAVMLWRFR
                     FPLKGAVDGTISLPIIVPEICLGVAFLMFFAGVGWPTDLAWPFNLGAITIAHITFCFP
                     FVTMVVRSRLASFNREQEEAAKDLGASEWQVFRDVLIPHIKPALVAGALLSFTLSLDD
                     FVITYFTSGPDTITFPVKVYSMVRFSVTPEVNAASTLLIIITVILTFVALKLQGVKAI
                     AETH"
     gene            5703..6833
                     /locus_tag="VN034_12710"
     CDS             5703..6833
                     /locus_tag="VN034_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV61516.1"
                     /translation="MTDPAHPIIQIRNVSKRFGKVTAVDNVSLEINAGEFFVLLGPSG
                     CGKTTLLRMIAGFELPTEGQILIDGQDMAGIPPNRRPVNMVFQSYAVFPHMSVADNVA
                     YGLKIAGVKGGEVKDRVAEALELVKLGGFETRMPDQMSGGQRQRVALARSLVMRPKVL
                     LLDEPLSALDAKLRAQMQFELSELQEKVGITFVTVTHDQDEALSMACRIGVINKGEVA
                     QLATPSDLYEFPANRFVADFVGSVNIFEGKLILDEPDRAAVDCPGLRKIYLNHGVTGP
                     HGADVWVALRPEKIYLHVPGAGKAVMAAAQDAPEGHNFARGTIKGMSYLGDITLFEIE
                     LETGARLRVSRPNLSRRDQEDFTWGDKVSMHWRADSPVVLLG"
     gene            complement(6839..7777)
                     /locus_tag="VN034_12715"
     CDS             complement(6839..7777)
                     /locus_tag="VN034_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HWV61517.1"
                     /translation="MPSGLFALLDDIATIAKVAAASIDDIGAAASKAGVKAAGVVVDD
                     TAVTPRYVTGFTPDRELPIIWRITKGSIFNKLVLILPALLLLSAVAQWAITPLLMIGG
                     AYLCFEGAEKVLHSLQKEKTTLAEDAAIVADQDHEEAMVKGAVRTDFILSAEIMVIAL
                     NEVIDEHFAMRAATLAVVAIAITIAVYGVVGLIVKMDDIGLHMAKEGNNARRAIGRGL
                     VSFMPKLLAALALIGTAAMLWVGGQIFLHGLDEYHIGNIGHGLHDFAHAVAGNLPGAG
                     VWEWLINAGGAGVFGLLLGGLIVGVLHFIPGKKAAH"
     gene            complement(7798..9171)
                     /locus_tag="VN034_12720"
     CDS             complement(7798..9171)
                     /locus_tag="VN034_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWV61518.1"
                     /translation="MPRNHDIAELRRLDVAHHLPAQADWAEIEALGGSRIITHAEGAY
                     ITDGDGHRILDAMAGLWCVNVGYGREELVEAAAAQMRELPFYNTFFKTATPPTVTLAA
                     KIASLTQNRLPHIFFNASGSEANDTVFRMVRHYWKLKGEPKRTIFISRWNAYHGSTVA
                     GVSLGGMKAMHAQGDLPIPGVEHVRQPYAYGEGQGMTEEEFCEACVAAIEDKILEVGP
                     ENCAAFIGEPVQGAGGVIIPPKGYWPKVEAVVRKYGLLLVSDEVICGFGRTGKMWGHE
                     TMGFTPDLMPMAKGLSSGYLPISATAVASHIVEVLKTGGDFVHGFTYSGHPVAAAVAL
                     KNIEIIEREGLVERTGSVTGPHLAKALATLDNHPLVGETRSIGLLGAVEIVADKETRA
                     RFGGAEGTAGPMARDACIANGLMVRGIRDSLVMCPPLIVTTEQIDEMVAIIRKSLDEV
                     MPKLRAL"
     gene            complement(9355..10095)
                     /locus_tag="VN034_12725"
     CDS             complement(9355..10095)
                     /locus_tag="VN034_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWV61519.1"
                     /translation="MSQRPVLGIIACNRLVGAEYAQAVMNRYAMAAMRHADCAALLIP
                     SLPDYMHADEIVGRLDGVLLTGSPSNIEPVRYGEDDAGEGPFDPDRDRMMRDLVRAVI
                     AAQRPLFGICRGFQDINVALGGTLRRDTAINGELLRHHTPDGTMFDAMFDHRHHVDLV
                     DGGMLASAYGATSLDVNSVHYQGVGRLADGLSVEARAPDGLVEAFSARPNGAPLIAVQ
                     WHPEWAVDGNAESQTYFHLLGRALRGGL"
BASE COUNT         1869 a         3357 c         3156 g         1857 t
ORIGIN      
        1 gcccctcgtc ttcgctcggg gatacggaga tggattgtgc gaaatcgagc gacaagcccc
       61 atgaccgacc cgctcgccaa cagcctttat gccgccaccg cgcacccatg gcgggcccgc
      121 gccttcgaag gcgacctcga atgtgacgtg gccgtgatcg gcggcggttt caccggcctc
      181 agtgccgcgc tcgcttgcgc cgagcgcggc ttttcggtga tcctgatcga acgcgagcat
      241 gtcggtttcg gcgcatcggg tcggaacggc ggacagttga ttcccggcct gcgctggacg
      301 gcatccgaac tcgaagccga attcggccgc gagcgcgccg atgcgctgtt cggcctctgc
      361 tggcgcgaca accgcgcgaa ggctcggatc gcgaaacatg gcatcgactg cgatctgaag
      421 gccgggcatc tggaggcggc gtggacgccg aaggatttcg acgccatgcg gcgcgaggcc
      481 gattatctcg cgaaccgctt cgactatcgc accgatattg tcgaaaaaaa cgagatggcc
      541 aggcatatcg tcagccccct ctatcatggc ggcatccacg atccgcaggc cggccatttc
      601 catccgctgg actatgcact ggggctggcc aaggcggcag aagcggcggg ggtccggatt
      661 tgggaaggcg aaccggtgcg ccggatcgcc gagttcagcg acgggtcgag cgaactcgtc
      721 accgaacggg cgatcgtcgg cgcgcgctat gtcatcgacg ccaccgacag ctggatcggc
      781 gacctcgaac ccgacctcgg ccgctacacc gtgccgatca tgaactataa tatcgcgacc
      841 gcgccgctcg cgaatgccga tacgctaatg cccaccgacg ccgcagtcgc cgacagccgc
      901 ttcgtgctca actatttccg cctttcggcc gacaagcggc tgatcttcgg cggcggcgag
      961 cgttactcac aaaccccgcc gcgcgacatc gcggccttcg tccggccctt catggcccag
     1021 gttttcccgc agatcgccga tgcgaagatc gattatggct ggggcggcgc cgtggcggtg
     1081 acgatgaacc ggttgccgca tatcgggcgg cgcgggaacg tctttttcgc gcacggcttt
     1141 tcggggcacg gcgcgctggt gacgacgctt gcgggcgaat taatcgccga agcgatggcg
     1201 ggaaccgccg aacgcttcga cattctcgcg aacctgccgt cgaagccctt cccgggcgga
     1261 aaatggctgc gccgcccgct cgccacgctg gggttgctct ggtatgcgct gcgcgaccgg
     1321 ttggggtgaa ggagcattta tgtcgaagtc atcgggcgtc atcgcgccgc gctccgaggc
     1381 ggaggccttc ttcaaggcga accccgacgt cgattcgatc gagatgatct acaccgacat
     1441 gggcggcgtt ccgcgcggca agcgcctccg ccagcacgag gttctcgccg tgtatgacag
     1501 cggccgcatg atgccgggct cgattaccgt cgtcgacatc accggacagg acacgatcga
     1561 aaccggcctc gtgtgggagg atggcgacgc cgaccgctcg atgaagccga tccccggcac
     1621 gctcgtccgc acgccatggg gcggcgacca cgccgcgcaa ttcctcgtcg gtttctacga
     1681 actcgacgga acgccgcacg acctcgaccc gcgccatgtg ctgggccgcg tgatcgaccg
     1741 ctataccgcc gatggcctga ccccggtgct cgcggtcgaa ctcgaattct acctcgtcga
     1801 tccgcgccgg gcgcgcgacg gccgcatccg ccccgcacgc cccggctaca gccgcgatac
     1861 cccgcgcaac gtcgaggttt atggcctgcg cgaactcgac gatttccgtc cctttttcga
     1921 cgcactttac gcggcgaccg acgtgcaggg tctgccgctc gaaagcgcga tctccgaatt
     1981 cgcgcccggt cagttcgaac tcactttgcg ccataaaccc gacgctttgc gtgcgtgcga
     2041 cgatgcgatc atgtacaagc ggcttgtgaa ggcgatcgcg cagcagcagg ggctcgaagc
     2101 gaccttcatg gcgaagccct tcgccgacca agcgggcagc ggcatgcaca tccacgtctc
     2161 ggtcgacgac gcggcgggca acaatatctt cgcgagcgac gatcccgaag gcacgcccgc
     2221 gctgcgccac gcgatcggcg gcatgatcgg cagcgtcggc gacggtttcg cgctcttcgc
     2281 gccgcacgcg aacagctacc gccggttcaa ggcgaacagc tatgcgcccg tcgcgccgac
     2341 gtggggcgtc aacaaccgca ccgtgtcgtt ccgcatcccc gccggcccgc cgccgagccg
     2401 ccacgtcgaa catcgcgcgt gcggcgccga cgccaacccc tatctcgccg tcgcggccgt
     2461 cctttccggc atgcaccacg gcatggcgaa ccagagcgac cccggccccg ccgtcgtcgg
     2521 caacggctac gaccgcgaca atagcggcga cgataagccg ccttccaact ggttcgccgc
     2581 cgtcgaccgc ttccatgcgt caaagctgat gcgcgattat ctcggcgaac ggttcgtcga
     2641 catgttcagc atcgtcaagc gcgtcgaaca ggacaattat ttcagcgtgg taccgacgct
     2701 cgattacgac tggtatcttc gcaacgcatg aaagtttcaa aaattcgcgg ctcagcgcaa
     2761 aaatcctctt tccaagccgc tcctattgag tcagcttgac gtcacataca gggaggccgg
     2821 ccatggattt caacgacctg atcaagcaaa cccgcgggcg ccgctcgctg ctccaggcgc
     2881 tcggcgtcgc ggcggtcggg atcagtttcg gcgggctcgc cgcgtgcagc aagcaggggg
     2941 gcaaaaccct cgcgaacggc gaggaagcca agctcaactt ctacaattgg gacacttata
     3001 tcggcgagaa tacgctcgat aatttcaagg aagccacggg cgtcgacgtc acgatggacc
     3061 tgttcgacag caacgacgtg ctgttcgcca aattcaaggc gggcaacccc ggttacgacg
     3121 tgatcgtgcc gtcgaacgac ttcgtcgagc ggatgtcgaa ggccggcatg ctgttgccgc
     3181 tcgaccatag ccttattccg aacaagcgga atatcgaccc cgcctatatc aacgtcgaat
     3241 acgacctcca gcgcaaattc tcgatgcctt acacctggct cgcgctcggc atcggctatc
     3301 gcaagtcgaa ggtgtcggcg acgcccgaca gctggaaagt gctgttcgac agccccgaat
     3361 atgccggccg catcgcttgg ctgtcggagg ccggcgacat gttccggctc tacggcaaat
     3421 atctcggcaa gtcggtcaac gcgctgaccc cggcggacat cgcgactata gaaaggatga
     3481 tgatcaagca aaagcccaat gtgaagaaat tccacgagga tgacgggcag gacctgctgc
     3541 tcaagggcga ctgcgacgtc gtgctcgaat ataatggcga catcgcgcag gcgatggtcg
     3601 aggacaagga catcgatttc gtcattccga aggagggcag ccagctcaac tcggacaatc
     3661 tctgcatccc gaagggcgcg ccgcacccga agaatgcgca cgctttcatc aactatatcc
     3721 tcgacgccaa tgtcgacaag catatcaccg agacgatcct ctatccgacg cccaacaccg
     3781 cggcgcgaaa gctgatgccc gacagttacc agaacaatcc ggtcatcttc ccgcccgccg
     3841 aggcgctggc gaaatgcgaa tatgcgcgct tcgaccccga gcttcagccg ctatacgaag
     3901 aggcgttcac ccgggttcgg gccgcataag ggtctgtagg ggcatcgggg gggtggccga
     3961 gcaggactgg aaaacgaaca ggggggcctt cgcggcggtg tcgctgccga cgctcttctg
     4021 ggtgatcctt ttcttcctcg tgccgatggc gatcgtctgg ctctacagct tcggcgaaaa
     4081 ccggggcctc accgagatcg aaatttcggg gacgctcgac aattacaagc gcgcgaccga
     4141 atggctctat ctcaccattt tcggcaagag cttcgcggtc gcggcgctcg tcaccttgat
     4201 ctgcctgatc gtcggctttc cggttgctat ggcgatcacc tttgcgagcg agaagtggcg
     4261 cccctggctg ctcctcgcca tcatgctgcc tttctggacc aacctcctca ttcgcaccta
     4321 tgcgctgatg atgctgctcg gcacccaagg cttcgcgaac aaggggctcg gtgcattgtg
     4381 ggacggggca agctggctca aggcgctcgt cgggctcgaa ccacttccga catgggaacc
     4441 ggtgcagctg ctcttcaata acttcgcggt cgtcttcggg cttgtctatg tccacctgcc
     4501 cttcatggtc ctgccgctct acgccgcgct cgaccgcctc gaccgcagcc tgatcgaggc
     4561 gagcctcgac ctaggcgcgg ggcatttccg cacgatcatg cgcatcgtcg tgccgctcgc
     4621 cgcgccgggt atcgtcgcgg gggtgatgat cacgctgatc cccgcattgg gcgcctatct
     4681 cacccccgac ctgatgggcg ggaccgacag ccagatgatc gccaatgtga tcgagcgcca
     4741 gttcaagaaa gcgaacgact ggcccttcgg cgccgcgctc tccttcctgc tcatatatgc
     4801 gatgttcgcg ctgatcgcga tccagtcgat gcgcagaaag gtgccggagg ttcactgatg
     4861 gcgctcttcg cccatacccc gcgcgcgccg ctcgaatata gccggacgct atggatgcgc
     4921 ctctgggtcg gcgcggtgat gctgttcctc tatgcgccgc tggtcgtgct ggtgatcttc
     4981 agcttcaacg acagcaagcg caacgtcgtg tggcgcggtt tcacaaccaa atattatgag
     5041 aaggcgctcg gcaacgacca gcttgtcgag gccctgctca actcgctcac catcgccgcg
     5101 ctcgcgacga tcgtcagcct catactcggc gcggtcgccg cggtaatgct ctggcgcttc
     5161 cgtttcccgc tgaagggtgc ggtcgacgga acgatctcgc tgccgatcat cgtccccgaa
     5221 atctgcctcg gcgtcgcctt cctgatgttc ttcgcgggcg taggctggcc gaccgacctc
     5281 gcctggccgt tcaacctcgg tgcgatcacc atcgcgcaca tcactttctg ctttcccttc
     5341 gtgacgatgg tcgtgcgttc gcgcctcgca agcttcaacc gcgaacagga ggaggccgct
     5401 aaggatctcg gcgcgagcga gtggcaagta tttcgcgacg tgctgattcc ccacatcaag
     5461 cccgcgctgg tcgcgggggc cttgctgtcc ttcacgctca gcctcgacga tttcgtcatc
     5521 acctatttca ccagcggccc cgacacgatc accttcccgg tgaaggtcta ttcgatggtc
     5581 cgcttctcgg taacgcccga ggttaacgcc gcatcgacgc tgctcatcat catcaccgtc
     5641 atcctgacct tcgtcgcgct caagcttcag ggcgtgaaag cgatagcgga aacccactga
     5701 ccatgaccga ccccgcccac ccgatcatcc agatccgcaa cgtgtcgaaa cgcttcggca
     5761 aggtcaccgc ggtcgacaat gtcagcctcg aaatcaacgc gggtgaattc ttcgtgctgc
     5821 tcggcccctc gggctgcggc aagacgacct tgctccgcat gatcgcgggg ttcgagctgc
     5881 cgaccgaggg ccaaatcctg atcgacgggc aggacatggc gggaatcccg ccgaacaggc
     5941 gcccggtgaa catggtgttc cagagctacg ccgtcttccc gcacatgagc gtcgccgaca
     6001 atgtcgccta tggtctcaag attgcgggcg taaagggtgg cgaggtcaag gaccgcgttg
     6061 ccgaggcgct cgaactcgtc aagctcggcg ggttcgagac gcgcatgccc gaccagatgt
     6121 cgggcggaca aaggcagcgc gtcgcgctcg cgcgcagcct cgtcatgcgt ccgaaggttc
     6181 tgctcctcga cgaaccgctc tcggcactcg atgcgaagct gcgcgcgcag atgcagttcg
     6241 aactcagcga attgcaggaa aaggtcggca tcactttcgt caccgtgacg cacgaccagg
     6301 acgaggcgct gtcgatggcg tgccgcatcg gcgtgatcaa caagggcgag gtcgcgcagc
     6361 ttgctacccc gtcggacctc tacgaatttc ccgcgaaccg tttcgtcgcc gattttgtag
     6421 gatcggtgaa tatcttcgag gggaagctga ttctcgacga gcccgacagg gcggcggtcg
     6481 actgtcccgg tcttaggaaa atatatttaa atcatggcgt tacaggaccg catggcgccg
     6541 acgtctgggt cgcgctgcgg ccggagaaga tttatcttca cgtccccggc gcgggcaagg
     6601 cggtcatggc cgcggcgcag gacgcacccg aaggtcacaa tttcgcgcgc gggacgatca
     6661 agggcatgtc ctacctcggc gacatcacgc tgttcgagat cgaactcgag accggcgcgc
     6721 gccttcgcgt ctcgcgcccg aacctctcgc gccgcgatca ggaggatttc acctggggcg
     6781 acaaggtcag catgcactgg cgcgccgaca gcccggtcgt tttgctgggg tgacaatctc
     6841 agtgagcggc cttcttcccc ggaatgaagt gcagcacacc gacgatcaga ccgccgagca
     6901 gcagcccgaa cacacccgcg ccgcctgcat tgatcagcca ttcccacacg cccgcgccgg
     6961 gcagattgcc cgcgaccgcg tgggcgaagt cgtgcagccc gtgcccgatg ttgccgatat
     7021 gatattcgtc gagcccgtgg aggaaaatct gtccgccgac ccacagcatc gccgcggtgc
     7081 cgatcagcgc gagcgccgcg agcaacttcg gcatgaaaga cacgagcccg cgcccgatcg
     7141 cgcggcgtgc attgtttcct tccttggcca tatgaagccc gatgtcgtcc atcttcacga
     7201 tcagcccgac gacgccatag accgcgatgg tgatcgcgat cgcaaccacg gcgagcgtcg
     7261 cggcgcgcat cgcgaaatgc tcgtcgatca cttcgttgag cgcgatcacc atgatttcgg
     7321 ccgacaggat gaagtcggtg cggacggcgc ccttcaccat cgcctcttca tgatcctgat
     7381 cggcgacgat cgcggcgtct tccgcaagcg tggtcttttc cttctgcagc gaatgcagca
     7441 ccttttcggc gccctcgaaa cagagataag cgccgccgat catcaagagc ggcgtgatcg
     7501 cccattgcgc taccgccgac aggagcagca atgcgggcag gatcagcacc agcttgttga
     7561 agatcgaccc cttggtgatc cgccagatga tcggcagttc gcggtcgggg gtgaagccgg
     7621 tgacgtagcg cggcgtcacc gcggtatcgt cgacgacgac gcccgccgcc ttgaccccgg
     7681 ccttcgacgc cgcggcgccg atatcgtcga tgctcgcggc ggcgaccttg gcgatcgtcg
     7741 cgatgtcgtc gagcagggca aaaaggccgg aaggcataag ggcttggtct ttcctgttta
     7801 gagcgcgcgg agtttgggca tcacctcatc caaggatttg cggatgatgg caaccatctc
     7861 gtcgatctgc tcggtggtga cgatcaaggg cgggcacatg accaggctgt cgcggatgcc
     7921 gcgcaccatc agcccgttcg cgatgcacgc gtcgcgcgcc atcggtccgg cggttccctc
     7981 ggcgccgccg aagcgggcgc gtgtttcctt gtcggcgacg atctcgaccg caccgagaag
     8041 gccgatcgag cgcgtttcgc cgacgagcgg atgattatcc agcgtcgcga gcgccttggc
     8101 gagatgcggg ccggtgacgc tgccggtgcg ctcgacgagc ccctcgcgtt cgatgatctc
     8161 gatattcttg agcgccaccg ccgcggcgac cggatggccc gagtaagtga aaccatggac
     8221 gaaatcgccg ccggtcttga gcacctcgac gatatggctc gcgaccgcgg tcgccgaaat
     8281 cgggagatag cccgacgaca gcccctttgc catcggcatc aggtcggggg tgaagcccat
     8341 cgtctcgtgc ccccacatct tgccggtgcg cccgaaaccg cagatgactt cgtccgacac
     8401 gagcagcagg ccatatttgc gcaccaccgc ctcgaccttc ggccaatagc ccttgggcgg
     8461 gatgatcacg ccgcccgcgc cttgcaccgg ctcgccgatg aaggcggcgc aattttcggg
     8521 gccgacctcg aggatcttgt cctcgatcgc agccacacac gcctcgcaga attcctcctc
     8581 ggtcatcccc tgcccttcgc cataggcata gggctggcgg acatgttcga cgccggggat
     8641 cggcaggtcg ccctgcgcgt gcatcgcctt catgccgccc agcgacacgc ccgcgacggt
     8701 cgagccgtga taggcgttcc agcgactgat gaagatggtg cgcttgggtt cgcccttcag
     8761 cttccaatag tggcgcacca tgcggaacac cgtgtcgttc gcctcgctgc ccgaggcgtt
     8821 gaagaagata tgcggcaagc ggttttgcgt gaggctcgcg atctttgccg cgagggtcac
     8881 cgtcggcggc gtcgcggtct tgaaaaaggt gttgtagaaa ggcagctcgc gcatctgcgc
     8941 cgccgccgcc tcgaccagct cttcgcggcc ataaccgaca ttgacgcacc aaaggcccgc
     9001 catcgcgtcg aggatgcggt ggccgtcgcc gtcggtgata taggcgccct cggcatgggt
     9061 gatgatgcgg ctgccgccga gtgcttcgat ttcagcccag tcggcttgtg cgggcagatg
     9121 gtgcgcgacg tcgaggcggc gcagttcggc gatgtcgtga ttgcggggca tgaatttgct
     9181 cctgagctag aagtccgttt gcatcgagcg aagtcgagat gcccctcggc cttgcactac
     9241 gacgatgggt gtctcgactt cgctcgacac gagcggagcg agagcctcgg ctcttcggtc
     9301 agggcgaaaa gctgatgcgc gcgagatagc ggtcgtagcg ggtgacgcgg cggttcataa
     9361 tccgcccctt aacgcgcgtc cgagaaggtg aaaataggtc tggctttccg cattgccatc
     9421 gaccgcccat tcgggatgcc attgcaccgc gatcagcggg gcgccattcg gccgcgcgct
     9481 gaacgcttcg acgaggccgt cgggcgcgcg cgcctccacc gaaagcccgt cggcgagccg
     9541 accgacgccc tgatagtgaa ccgaattcac gtcgagcgag gtggcgccat aggccgacgc
     9601 gagcatccct ccatcgacca gatcgacatg gtggcgatga tcgaacatcg catcgaacat
     9661 tgttccgtcg ggcgtatgat ggcgaagaag ctcgccgttg atcgccgtgt cgcggcgcag
     9721 cgtcccgccg agcgcgacat tgatgtcctg aaacccgcgg caaatcccga acagcgggcg
     9781 ctgcgcggca atcaccgcac gaacaagatc gcgcatcatg cgatcgcggt cggggtcgaa
     9841 cggcccctcg cccgcatcat cctcgccata gcgcacgggc tcgatatttg acggcgagcc
     9901 ggtgaggagc actccgtcga gccgcccgac gatctcgtcc gcgtgcatat agtcgggaag
     9961 cgacgggatc agcagcgccg cgcaatcggc atggcgcatc gccgccatcg catagcggtt
    10021 catcaccgcc tgtgcatatt cggcgcctac cagtcggttg caagcgatga tgccgaggac
    10081 gggacgttgc gacatgacgc cacgctgccg tgtttcgggg aaaggcgcca gcgaaatatg
    10141 gtgtgggggg tggcgtcggt tttgggggtg gtttccagac ctcccctata accgtcgccc
    10201 ccgcgaaggc gggggccgct agcggcctta ctcggcgac
//
LOCUS       DATMSU010000478         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_74637, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000478 DATMSU010000000
VERSION     DATMSU010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74637"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VN034_12730"
     CDS             complement(<1..279)
                     /locus_tag="VN034_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61520.1"
                     /translation="MTKLISLSLIAAATLAGCASTGPKSADPEPGVTTLDVRRTAASA
                     KALDEIAAAYLRLSLEIGTHEPGYIDAYYGPPELKKAAEAAPRDKPALL"
     gene            complement(290..1564)
                     /gene="nhaA"
                     /locus_tag="VN034_12735"
     CDS             complement(290..1564)
                     /gene="nhaA"
                     /locus_tag="VN034_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HWV61521.1"
                     /translation="MARSFPPRSALRDFLESESAGGMLLIFAAILAMIVANSAFAESY
                     EHAIHAVTGPILTDKLGPMTVHLWINDGLMAIFFLLVGLEIKREFVDGRLASWDRRRL
                     PFIAAAAGMAVPAALYMLFAGSEPGLAQGWAIPAATDIAFAMGVLALLGKRAPTSLKL
                     FLVTVAIVDDMGAVAIIALFYTAKINVMALGAAAAILAVMFAMNRSGVKSLILYLLMF
                     LLLWYAMLLSGVHATIAGVLAAMTIPFERTPGAPDSQTSPLHRLEHALHPWVAFAIVP
                     LFGFANAGVDVRGLGIDQIFAPLPLGIAAGLFLGKQIGIFGSVWLAVKLGIAGRLRGA
                     TWLQVYGVAMLCGIGFTMSLFIGGLAFPGDALLVEEAKIGILMGSLAAALAGFAVLRF
                     APPHPQHEAVEQASNGEIAADGDVRDTSEAHG"
     gene            1702..3126
                     /locus_tag="VN034_12740"
     CDS             1702..3126
                     /locus_tag="VN034_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HWV61522.1"
                     /translation="MGLSTAWLEWLVLGAGHELMLFASVGILLIGLDDLLFDALWLAT
                     HRRDPAPVPNAPPIDGRFAIFVPAWDEAEVLPAMLHRTLAAWQGEDFRLYVGCYPNDA
                     ATLFAVSPLVARDRRLRLVIGGHEGPTTKGDNLNRLWAALGEDERAEGMRFAAIVLHD
                     AEDHVHREELALYRRYLGEDAMVQIPVVPLIKREERWVGGHYGDEFAEAHGKELVVRS
                     RLGLPLPSAGVGCALTRSTLALLAIERGGEPFCCDSLTEDYEIGMLIGAYGLGTRFID
                     TVGPTGDRIVSRGAFPGEVEKAVRQKSRWIAGISLAGWDHLGWPGSRQVDAGLSANRA
                     WLARWMLWRDRRAPLAALILLAAYAGAILTGLGLAGQLLLGWDAIVIGEGMRLLLAVN
                     LLLLCWRMGMRGHFTARWYGLGEALISVPRAFVANVIAILAARRAVTLYWRMLRSGEV
                     VWDKTDHCQSEPVHQDALVRRAAR"
     gene            3126..>3420
                     /locus_tag="VN034_12745"
     CDS             3126..>3420
                     /locus_tag="VN034_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61523.1"
                     /translation="MARRIGARRDAPALRFLLLCIGGWIALRIMMTWNPAMSVPPDVP
                     AMPWPPPPSFVTEGGLADSRSFAHRIPAKAMSAQGAGPLRALAAPRPDRPNSAP"
BASE COUNT          554 a         1088 c         1191 g          587 t
ORIGIN      
        1 gagcagcgcg ggcttgtcgc gcggcgccgc ttcggccgct tttttgagct cgggcgggcc
       61 gtagtaggcg tcgatatagc ccggctcgtg cgtcccgatt tcgaggctca gccgcagata
      121 ggcggcggcg atttcgtcga gcgccttggc cgaagcggcg gtgcggcgca cgtcgagtgt
      181 ggtgacgccg ggctcggggt ctgcactttt cggcccggtg gaggcgcagc ccgcgagggt
      241 cgcggcggcg atcagcgaca atgaaatcag cttggtcatg cccggctgct tagccgtggg
      301 cttcggaggt gtcacgcaca tcgccgtcgg cggcgatttc gccgttggaa gcctgctcga
      361 ccgcctcatg ctgcgggtgc ggcggggcga agcgcagcac ggcaaagccg gcgagcgccg
      421 cggcaagcga gcccatcagg atgccgatct tcgcttcctc gacgagcagc gcgtcgccgg
      481 ggaaagcgag cccgccgatg aacaggctca tcgtgaaacc gatcccgcac agcatcgcga
      541 cgccatagac ctgaagccag gtcgcgccgc gcagccggcc cgcgataccg agtttgaccg
      601 cgagccagac gctgccgaaa atgccgatct gcttgccgag gaacagcccg gcggcgatgc
      661 cgagcggcaa tggcgcgaaa atctggtcga tcccgaggcc gcggacatcg acgccggcgt
      721 tggcgaagcc gaacagcggg acgatcgcga acgcgaccca ggggtgcagt gcatgttcga
      781 gccggtgcag cggcgaggtc tggctgtcgg gcgcgcccgg cgtgcgctcg aacgggatcg
      841 tcatcgccgc gagcacgccc gcgattgttg catggacccc cgaaagcagc atcgcgtacc
      901 agaggaggag gaacatcagc agatagagga tgaggctctt cacccccgag cggttcatcg
      961 cgaacatgac ggcgaggatc gccgccgccg caccgagcgc catgacattg atcttggcgg
     1021 tgtagaagag cgcgatgatc gccaccgcgc ccatgtcgtc gacgatcgcg acggtgacga
     1081 ggaacagctt gagcgaggtc ggcgcgcgct tgccgagcag cgcgagcacc cccatcgcaa
     1141 aggcgatgtc ggtcgccgcc gggattgccc agccttgggc aagtccgggt tcgctgccgg
     1201 cgaacagcat gtagagcgcc gccggcaccg ccatacccgc cgccgccgcg atgaagggca
     1261 gccgccgccg gtcccaactc gcgagccggc cgtcgacgaa ctcgcgcttg atctcaagcc
     1321 cgacgagcag gaaaaaaatc gccatcagcc cgtcgttgat ccacagatgg accgtcatcg
     1381 ggccgagctt gtcggtgaga attgggccgg tcaccgcgtg aatcgcgtgc tcataactct
     1441 ccgcgaaggc cgaattggcg acaatcattg ccaaaatggc tgcaaaaatg agcagcatgc
     1501 cgccggcgct ctcactttca agaaaatcgc gcaatgcgga gcgaggcgga aaactgcggg
     1561 ccatggatta gttatttcct tattttgtca gcgaatttga cgattgggca gctatttggc
     1621 ccagacttta caaatcgggc ctgttcacta gcacttagat ggaggtgaca ttaggtcatc
     1681 ggggggagcg gggctagcaa attggggctt tcgaccgcat ggctggaatg gctggtgctg
     1741 ggcgccggcc atgaactgat gctgttcgca tcggtcggca tattgctgat cgggctcgac
     1801 gacctgctgt tcgacgcctt gtggctggcg acgcaccgcc gcgacccggc gccggttccg
     1861 aacgcgccgc cgatcgacgg gcgcttcgcg atcttcgtgc ccgcgtggga cgaagccgaa
     1921 gtgcttccgg cgatgctgca ccggacgctt gcggcatggc agggggagga tttccggctt
     1981 tatgtcggct gctatccgaa cgacgccgcg acgctgttcg ccgtgtcgcc gttggtcgcg
     2041 cgcgaccggc ggctgcggct ggtgatcggc ggccacgagg ggccgacgac caagggcgac
     2101 aatctcaacc ggctgtgggc ggcgctgggc gaggacgaac gcgccgaagg catgcgtttt
     2161 gccgcgatcg tccttcacga tgccgaggat cacgtccacc gcgaggagct cgcgctttat
     2221 cgccgctatc tcggcgaaga tgcgatggtg caaattcccg tcgtgccgct gatcaagcgc
     2281 gaggaacgat gggtcggcgg gcattatggc gacgaattcg ccgaggcgca cggcaaggag
     2341 ctggtcgttc gttcgcgcct tggtctgccg ttgccctcgg cgggcgtcgg ctgcgcgctg
     2401 acacgaagca cgctcgcgct gctggcgatc gagcgcggcg gcgagccctt ttgctgcgac
     2461 agcctgaccg aagattatga gatcgggatg ctgatcgggg cctatggcct tggcacgcgc
     2521 ttcatcgaca cggtcgggcc caccggcgac cggatcgtgt cgcgcggcgc ttttcccggc
     2581 gaggttgaaa aggcggtgcg gcaaaaatcg cgctggatcg cgggaatctc gcttgcggga
     2641 tgggatcacc tcggttggcc gggttcgcgg caggtcgacg ccgggctctc cgcgaaccgc
     2701 gcctggctcg cgcgctggat gctgtggcgc gatcgccgtg cgccgctcgc agccctcatc
     2761 ctcctcgccg cctatgccgg ggcgatcctg accggactgg gcctggcagg gcagttgctt
     2821 ctcggctggg atgcgatcgt catcggagag ggcatgcggc tgctcctcgc cgtcaatctg
     2881 ctgctgctct gctggcgaat gggaatgcgc ggtcatttta cggcgcgctg gtatggcctt
     2941 ggcgaggcgc tgatctcggt gccgcgcgcc ttcgtcgcga acgtcatcgc gattctcgcc
     3001 gcgcgccgcg cggtcaccct ttactggcga atgctgcgat cgggcgaagt ggtgtgggac
     3061 aagaccgacc attgccaaag cgagccggtt catcaggatg ccttggtccg gagggccgcg
     3121 cgatgatggc gcggcgcatc ggcgcgcgcc gcgatgcccc ggcgctgcgc ttcctgctgc
     3181 tgtgcatcgg cggctggatc gccctgcgca tcatgatgac gtggaatccc gccatgtcgg
     3241 tgccccccga cgtcccggca atgccgtggc cgccgcctcc atcctttgtc accgaaggcg
     3301 gccttgcgga cagccggtcc tttgctcatc gcataccggc gaaggccatg tcggcgcaag
     3361 gcgcgggccc gctccgcgct ttggcggcac cgcggccgga ccgccccaat tccgccccct
//
LOCUS       DATMSU010000479         4070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_102757,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000479 DATMSU010000000
VERSION     DATMSU010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102757"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN034_12750"
     CDS             <1..270
                     /locus_tag="VN034_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWV61524.1"
                     /translation="IWGGAQDWHLGDHLADAAKRAGLDLAELDEEAARDAEALDNEIA
                     GNQVALEIAGHWGVPTLVFEGEPFFGQDRIEMAKWRMEQKGLLPR"
     gene            315..1067
                     /locus_tag="VN034_12755"
     CDS             315..1067
                     /locus_tag="VN034_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWV61525.1"
                     /translation="MTIEPGYFEARDGVRLAWRETGEGRPLILLHGLFSSGEVNWIKF
                     GTAARIARDGYRVIMPDLRVHGSSAAPHEEEFYPPDVLVHDLGDLIAHLDLADFDLGG
                     FSLGARTSARAVVAGMKPGRLILGGMGLAGLAGWQRRGRFFKRVIAEYESAKRGDDTW
                     LSIQFMKTMKVDRIAAGYLLDTFTDTTPEMLAALTMPTLVVCGEQDHDNGSAEELVAA
                     LPDARLRAIPGTHMSSVTEPELGEAIAAFLAT"
     gene            1141..2982
                     /locus_tag="VN034_12760"
     CDS             1141..2982
                     /locus_tag="VN034_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HWV61526.1"
                     /translation="MKAMISTLSLALSLAVATPAFAQSAPAAAPAAAAPTAADADAFI
                     AAVEKDLFDYTVEASQVNWVNATYITEDTDAMAARINAVGTEKSVKYALEAAKYMNVA
                     GLSADTKRKLDILRNGIVLPAPTTPGAATELNQIATNLQSQYGKGRGTLNGKEISGSD
                     IEAEMGNLEHTPAEYAEMWTSWHDNVGAPMKDDYARMVGIANEGAKELGFANTGAMWR
                     SGYDMPPEEFAKLTEKIWQDMKPLYVALHTYVRWKLNEKYGDAVQPKTGPIRADLLGN
                     MWAQEWGNIYPLVAPAGAGDLGYDIGDLLTAQGKTPLDMVKAGENFYSSLGMAPLPET
                     FWKRSLFTKPADREVICHASAWDIDNKDDIRIKMCIKVNADDFVTIHHELGHNYYQRA
                     YNKQPFLYLNGANDGFHEAIGDFVALSITPQYLVDIGLLDKAKVPSADKDIGLLLRQA
                     MDKVAFLPFGLLVDRWRWGVFDGSIQPADYNKAWTELRTQYQGIVPPGERPANAFDAG
                     AKYHIPGNTPYTRYFLARVLQFQFYQAACKQAGWKGPLHRCSFYGNKEVGAKLNAMLE
                     MGASKPWPDALEAFTGNREMSGKAMADYFAPLKTWLDAQNKGKPSGW"
     gene            complement(3037..3363)
                     /locus_tag="VN034_12765"
     CDS             complement(3037..3363)
                     /locus_tag="VN034_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61527.1"
                     /translation="MSIHEEYDNVFKALSSHVRRQILDDLKDQPLTTGTLCGNFAHLD
                     RCTVMQHLKVLEEAGLVIAERRGRTRWNHLNAIPIQDIHDRWIGPHAAAATARLARFK
                     QSVEAQ"
     gene            3413..3895
                     /locus_tag="VN034_12770"
     CDS             3413..3895
                     /locus_tag="VN034_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV61528.1"
                     /translation="MELKFRVAARIAKPVHEVFEAVADPAKLSNYFTTGGAQGRLETG
                     ATVTWDFHDFPGAFPVWVIEVVEDQKIVLEWQANEGEAHNLEGSELKDTDYRTRVTMR
                     FKGLEDGRTLVEIAEEGWRENQGALSASYGNCQGWSQMLCALKMWIEHGINLREGMYQ
                     "
BASE COUNT          778 a         1240 c         1354 g          698 t
ORIGIN      
        1 atctggggcg gcgcgcagga ctggcatctc ggcgatcatc tcgcggacgc ggcgaagcgc
       61 gcggggctcg acctcgccga actcgacgaa gaggcggcgc gcgacgccga ggcgctcgac
      121 aatgaaatcg ccggcaatca ggtcgcgctc gaaatcgcgg gccattgggg cgtgccgacc
      181 ctcgtgttcg aaggcgagcc ctttttcggg caggaccgga tcgagatggc gaagtggcgc
      241 atggagcaaa aggggcttct cccgcgctga tcggcgcttg cccgatcgcc cttcaaggag
      301 gcatagcgcg tcctatgacg atcgaacccg gatatttcga agcgcgcgac ggcgtgcggc
      361 tggcgtggcg cgagacgggc gaaggccgcc ccctgatttt gctgcacggc ctgttttcga
      421 gcggcgaggt caactggatc aagttcggca ccgcggcgcg catcgcccgt gacggctatc
      481 gggtgatcat gcccgacctg cgcgtccacg gatcgagcgc cgcgccgcac gaggaggaat
      541 tctacccgcc cgacgtgttg gtgcacgatc tgggggatct gatcgcgcat ctggacctcg
      601 ccgacttcga tctcggcggc ttctcgctcg gggcgcggac gagcgcgcgc gcggtggtcg
      661 cggggatgaa gccggggcgg ctgatcctcg gcggcatggg actcgccggg ctcgctggct
      721 ggcagcggcg cgggcgcttc ttcaagcgcg tgatcgccga atatgaaagc gcgaagcgcg
      781 gcgacgacac ctggctgtcg atccagttca tgaagacgat gaaggtcgac cgcatcgcgg
      841 cggggtatct gctcgacacg ttcaccgaca cgacacccga gatgctcgcg gcgctgacga
      901 tgccgaccct cgtcgtctgc ggcgagcagg accacgacaa tgggtcggcc gaagaacttg
      961 tcgcggcctt gcccgacgcg cggctgaggg cgattcccgg aacgcatatg tcgagcgtga
     1021 ccgagcccga gctgggggag gcgatcgccg cctttctcgc gacttgaccg gactcgcgcc
     1081 gagcgcctag ctgttcgtat gcgccgtaga gcgctgatag aacatccaga ggacgcaccc
     1141 atgaaagcca tgatttcgac gctgtcgctt gccctgtcgc tcgcggtggc gacccccgcc
     1201 tttgcgcaga gcgcgcccgc cgctgccccc gcggccgccg cacccaccgc cgctgacgcc
     1261 gacgccttta tcgcggcggt cgaaaaggat ttgttcgact atacggtcga ggcgagccag
     1321 gtgaattggg tcaacgcgac ctatatcacc gaggacaccg acgcgatggc ggcgcggatc
     1381 aacgcggtcg gcaccgaaaa gtcggtcaaa tatgcgctcg aggccgcgaa atatatgaat
     1441 gtcgcggggc tcagcgccga caccaagcgc aagctcgata ttttgcgcaa cggcatcgtg
     1501 ctgcccgcgc cgacgacacc cggcgccgcg accgagctca accagatcgc gacgaacctt
     1561 cagtcgcaat atggcaaggg ccgcggcacg ctgaacggca aggaaatttc gggttctgat
     1621 atcgaggccg agatgggcaa tctcgagcac acacccgccg aatatgccga aatgtggacg
     1681 agctggcacg acaatgtcgg cgcgccgatg aaggatgatt atgcgcgcat ggtcggcatc
     1741 gccaacgagg gcgcgaagga actgggcttc gcgaacaccg gcgcgatgtg gcgctcgggc
     1801 tatgacatgc cgcccgagga attcgccaaa ctgaccgaga agatctggca ggacatgaag
     1861 ccgctttacg tggcgctcca cacctatgtt cgctggaagc tcaacgaaaa atatggcgat
     1921 gcggtgcagc ccaagaccgg cccgatccgc gccgatctgc tcggcaatat gtgggcgcag
     1981 gaatggggca atatctatcc gctcgtcgcg ccggcgggtg cgggcgatct cggttacgac
     2041 atcggcgacc tgctcaccgc gcagggcaag actccgctcg acatggtcaa ggcgggcgag
     2101 aatttctatt cgtcgctcgg catggcgccg ctgcccgaga ccttctggaa gcggagcctg
     2161 ttcaccaagc ccgccgaccg cgaagtcatc tgccacgctt cggcgtggga tatcgacaac
     2221 aaggacgata tccgcatcaa gatgtgcatc aaggtaaatg ccgacgactt cgtcaccatc
     2281 caccacgaac tcgggcacaa ttattatcag cgcgcctata acaagcagcc gttcctctat
     2341 ttgaacggtg cgaacgacgg cttccacgaa gcgatcggcg atttcgtcgc attgtcgatc
     2401 acgccgcaat atctggtcga catcggcctg ctcgacaagg cgaaggtgcc gagcgcagac
     2461 aaggatatcg gcctcttgct gcggcaggcg atggacaagg tcgccttcct gcccttcggc
     2521 ctgctcgtcg accgctggcg ctggggcgtc ttcgacggct cgatccagcc cgccgactac
     2581 aacaaggcgt ggaccgagct tcgcacccaa tatcagggaa tcgtcccgcc gggtgagcgg
     2641 ccggcgaacg cgttcgacgc gggtgcgaaa tatcatatcc cggggaacac gccctatacg
     2701 cgctatttcc tcgcgcgcgt gctgcagttc caattctatc aggcggcgtg caagcaggcg
     2761 ggctggaagg ggccgctcca ccgctgttcc ttctatggca acaaagaggt cggcgcgaag
     2821 ctcaatgcga tgctcgaaat gggcgcgtcg aagccgtggc ccgatgcgct cgaagccttc
     2881 accggcaacc gcgaaatgtc gggcaaggcg atggccgact atttcgcgcc gctgaagaca
     2941 tggctcgacg cgcagaacaa gggcaagccg tcgggctggt gagcagcgat ttttagggaa
     3001 gacgaagagg ccggggtggc gacgccccgg cctttttcat tgcgcctcga ccgattgttt
     3061 gaagcgcgcc agccgcgcgg tcgccgcagc ggcgtgcggg ccgatccagc ggtcgtggat
     3121 atcctggatc ggtattgcgt tgaggtggtt ccagcgggtg cggccgcggc gttcggcgat
     3181 gacgaggccg gcttcctcca gcaccttcag atgctgcatc accgtgcagc ggtcgagatg
     3241 cgcgaagttg ccgcacagcg tgccggtggt cagcggctgg tccttgaggt cgtcgaggat
     3301 ctggcgacgg acgtgcgacg acaaggcctt gaaaacattg tcatattctt cgtggattga
     3361 catgttataa atttataaca taaaatccgc ccgactcaag cggagaatcg acatggaatt
     3421 gaaattcagg gtcgcggcgc ggatcgcgaa gccggtgcac gaggtgttcg aggcggtcgc
     3481 cgaccccgcg aagctgtcga actatttcac cacgggcggc gcgcagggcc ggctcgagac
     3541 cggcgcgacg gtgacatggg acttccacga ctttcccggc gcctttccgg tctgggtgat
     3601 cgaggtggtc gaggatcaga agatcgttct cgaatggcag gcgaacgagg gcgaggcgca
     3661 caatctcgag ggcagcgagc tcaaggatac cgattatcgc acgcgcgtga cgatgcgctt
     3721 caaggggctc gaagacggcc gcacgctggt cgaaatcgcc gaggaggggt ggcgcgagaa
     3781 tcagggcgcg ctgagcgcct cctacggcaa ttgccagggc tggtcgcaga tgctctgcgc
     3841 gctcaagatg tggatcgagc acgggatcaa ccttcgcgag ggcatgtatc aatagctcga
     3901 caatttgtgt gccggagcat gatgttcgaa gtgctgcggg cgggacgctg acgtccagaa
     3961 tcgaccggtt gcggacctac cttctcccct cccttttagg gaggggccgg gggtgggtag
     4021 ccgccgaagg cggcgttctt ttccggctcg ctgcgctcgc gcacccaccc
//
LOCUS       DATMSU010000480         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_168706,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000480 DATMSU010000000
VERSION     DATMSU010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168706"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VN034_12775"
     CDS             complement(<1..1098)
                     /locus_tag="VN034_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HWV61529.1"
                     /translation="MAKDVFILGGAQSDFARNLEREGGGLFELFRDVAEAAFVATGIE
                     PKEVETAHVGNFVGELFAGQGQLGGFFGHVHPDLAGIPASRHEAACASGSIAILAAAA
                     EIEAERYGLALVLGIELMRNVPGQRAAEYLGAAAWAGREAQEARYLWPYMFARVAEEY
                     EERFGLDRAYLRGISANNFANAKRNPNSQTRGWAITDAHLGENDEVNPLIEGSLRKSD
                     CGQVTDGAAAIFLASVEVAEVYAQRRGISIDSIPRIKGWGHATAPLLYSTKVRDSRGQ
                     DFVFPSVRKAMMDALRRAEMPDIYACDGVEVHDCFSITEYMAIDHFGITKPGESWRAV
                     EDGTIALGGKLPVNPSGGLIGLGHPVGATGVR"
     gene            complement(1100..2791)
                     /locus_tag="VN034_12780"
     CDS             complement(1100..2791)
                     /locus_tag="VN034_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HWV61530.1"
                     /translation="MIVAGEDKRRRHRESGWWGDQTFADLFAANAAAHPERLALVDAP
                     NRGDFAFGEPQRLSYAELAAEIDRIAGALVVAGIGKDDVLLVQLPNISEFVALYFAAA
                     QIGAIVSPAAVQYRSHELKGMIGVVEPKAFVCATMVKGCDHVGVAQPLLDGIQLMTFG
                     PTPPAGALDLSTVRGDEAALAAHVAANPVDADDIFTICWTSGTTGVPKGVPRSHNHWI
                     AVAGAGYEAMKVGPGDVLLNPFPLINMASIGGITMCWLTSAGTMALHHPFDPGVYLKQ
                     IATERPSLTIAPPAVLNMLLQNEALLASVDLSSLRVIASGSAPLAPAMVRGFQEKLGI
                     IIVNVFGSNEGMSFITGEGDMPDPDKRASLFPRRGTYQRPYGEGRAPNIESRLVPPGG
                     GDPIKEDGVSGELQIRGPTLFEGYHNAPDRTAEAFTDDGWFRTGDLFEIAEGGDFYRF
                     VGRCKDLIIRGGVNISPEEIDQLLGGHPSLAEACTFSLPCPTMGERIGLAYVPRGDGD
                     VGIDEVTDYLREKDLAVFKLPERLFRFDALPRNVTNKVMRSEVREQALATLEKEA"
     gene            complement(2793..4289)
                     /locus_tag="VN034_12785"
     CDS             complement(2793..4289)
                     /locus_tag="VN034_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWV61531.1"
                     /translation="MTAELVDTITTTIQPNDHPYMKGAWRPTYNEWNAIFADGDAEVI
                     GNIPTDIEGVYVRTGENQIHEPIGRYHPFDGDGFIHSISFKDGRASYRSRFVRTKGFE
                     AEKEAGRSLWAGLMEPPHKSTRHGWGAQEWLKDSSSTDVAIHAGKIISTFYQCGEAYR
                     LDPFTLEQYGTESWVPLDGISAHCKVDLATGELMFFNYSKHAPYMHYGVVGADNRLKH
                     YIPIPLAGPRLPHDMAFTKNYTILNDMPLYWNEELLERNLHVVQFHPDEKTRFAIIPR
                     HGNPEDIRWFEAEPTYTLHWLNAWEEGDEIILDGYYQEEPMPKSYPNAPDGLERMMAY
                     LDQGLLKPRLHRWRFNLKTGETTEERLDDRDLEFGMFNHRYAGKPYRYAYSAIPEPGW
                     FLFRGLVKHDLSAGTSEEYEFGPGRFGSEAPFAPRIDAKDEDDGYLVSFIADLETDKS
                     ECVLIDAKNVAAGPVCRIILPERICSGTHTVWASGDDIGMGPNSVLAA"
     gene            complement(4286..>4791)
                     /locus_tag="VN034_12790"
     CDS             complement(4286..>4791)
                     /locus_tag="VN034_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWV61532.1"
                     /translation="TGATAGFAFVEEVRQFWDGPLVLGGAISTGHAIRAAEILGADFA
                     YLGTSLIACAESMAVPGYKEMIVAAGAEDIIPSKGITGVTANWLKSSLIAAGYDPMNM
                     PEDKRPNFSDAQDDAKAWKNVWSAGQGVGAVRGVEPVADIVDRLVKEYDAAAARPRFA
                     PAEGVPA"
BASE COUNT          816 a         1540 c         1552 g          883 t
ORIGIN      
        1 ccgcactccg gtggcaccta cgggatgccc aagcccaatc agcccgcccg atggattgac
       61 cggcaatttg ccgcccagcg caatcgtccc atcctcgacc gcacgccagc tttcgcccgg
      121 ttttgtgata ccgaaatgat cgatcgccat atattcggtg atcgagaagc aatcatgcac
      181 ctcgaccccg tcgcacgcat agatgtcggg catctcggcg cgccggagcg cgtccatcat
      241 cgccttgcga acactaggaa aaacaaagtc ttggccgcga ctatcccgaa ccttggtcga
      301 atagagcagc ggcgccgtgg catggcccca tcccttgatc cgcggaatgc tgtcgatcga
      361 aatcccgcga cgctgcgcgt aaacttcggc aacttccacc gaagccagaa agatcgccgc
      421 cgcgccgtcg gtgacctgcc cgcaatcgga cttgcgcagg ctcccttcga tcagcggatt
      481 gacctcgtca ttctcgccga gatgcgcgtc ggtgatcgcc cagccgcgcg tctgcgaatt
      541 gggattgcgc ttggcattgg cgaagttgtt cgccgaaatc ccgcgcagat atgcacggtc
      601 gaggccgaag cgctcctcat attcctcggc gacgcgcgca aacatgtatg gccacaggta
      661 gcgcgcctcc tgcgcttcgc gccccgccca ggccgcagcg ccgagatatt cggcggcgcg
      721 ctggcccggc acgttgcgca tcagttcgat gccgagcacc agcgcgagac catagcgttc
      781 ggcctcgatc tcggcggcgg cggcgaggat cgcgatactg cccgacgcgc aggccgcctc
      841 gtgccgcgat gcggggatgc ccgcgaggtc gggatgcaca tggccgaaga agccgccgag
      901 ctggccttgc cccgcgaaga gttcgccgac gaaattgccg acatgcgcgg tctcgacttc
      961 cttgggctcg atccccgtcg cgacgaacgc cgcttcggcg acgtcgcgga acagctcgaa
     1021 cagcccgccg ccctcgcgtt cgaggttgcg cgcaaaatca ctctgcgcgc cgccgaggat
     1081 gaagacgtct ttcgccatgt caggcttcct tttcgagagt ggcgagcgcc tgttcacgca
     1141 cttcgctgcg catcaccttg ttggtgacgt tgcgcggcaa cgcatcgaag cggaacaggc
     1201 gttcgggcag tttgaacacc gcgagatcct tttcgcggag atagtcggtg acttcgtcga
     1261 tgccgacgtc gccgtcgccg cggggcacat aggcgaggcc gatccgctcg cccattgtcg
     1321 gacaggggag cgagaaggtg caggcttcgg cgagcgatgg gtggccgccg agcaactgat
     1381 cgatctcctc gggcgagata ttcaccccgc cacggatgat cagatccttg cagcgcccga
     1441 cgaagcggta gaagtcaccg ccctcggcga tctcgaacag gtcgccggtg cggaaccagc
     1501 cgtcgtcggt gaaagcctcg gcggtgcgat cgggggcgtt gtggtagccc tcgaacaagg
     1561 tcggtccgcg gatctggagt tcgcccgata cgccgtcttc cttgatcgga tcgccgccgc
     1621 ccggcgggac aaggcggctt tcgatattgg gcgcgcggcc ctcgccatag gggcgctgat
     1681 aggtgccgcg acgcgggaac aggctcgcgc gcttgtcggg gtcgggcatg tcgccttcgc
     1741 cggtgatgaa gctcatcccc tcgttcgacc cgaagacgtt gacgatgatg atgccgagct
     1801 tttcctgaaa accgcgcacc atcgcgggcg cgagcggcgc cgagcccgag gcgatgacgc
     1861 gcaggctcga caggtcgacc gacgcgagca aggcctcatt ctgcagcagc atgttgagca
     1921 ccgcgggcgg cgcgatcgtc aggctcggcc gctcggtcgc gatctgcttc aaatagacgc
     1981 cgggatcgaa ggggtggtgg agcgccatcg tccccgcgct ggtcagccag cacatggtga
     2041 taccgccgat gctcgccatg ttgatgagcg ggaaggggtt gagcaataca tcgcccgggc
     2101 cgaccttcat cgcctcgtac cccgcgcccg cgaccgcgat ccagtgattg tggctgcgtg
     2161 gtacgccctt ggggacgccg gtggtgcccg aggtccagca gatggtgaag atatcgtcgg
     2221 cgtcgacggg gttcgccgcg acatgggcgg cgagggcggc ttcgtcgccc ctaacggttg
     2281 agaggtccaa cgcaccggcc ggcggagtcg ggccgaaggt catcagttga atgccgtcga
     2341 gtagtggctg tgccacgccg acatggtcgc agcccttgac catcgtcgca caaacaaacg
     2401 ccttgggctc gacgacgccg atcatgcctt tcagctcatg gctgcgatat tgcaccgccg
     2461 ccgggctgac gattgcgccg atctgcgcgg cggcgaaata gagcgcgacg aattcgctga
     2521 tgttgggaag ctggacgagc agcacatcgt ccttgccgat gcccgcgacg accagcgcgc
     2581 ccgcgatccg gtcgatctcg gcggcaagtt cggcatagct cagccgctgc ggctcaccga
     2641 acgcaaagtc gccgcggttc ggcgcgtcga ccagcgcgag gcgttcggga tgcgccgccg
     2701 cattggcggc gaacaggtcg gcgaaggtct gatcgcccca ccagccgctt tcccgatgcc
     2761 gccgcctctt atcctctccc gcgacgatca tctcaggcgg ccagcacgct gttcggaccc
     2821 atgccgatat cgtcgccgct cgcccagacc gtatgcgtgc ccgagcagat gcgttcgggc
     2881 aggatgatcc ggcacaccgg cccggccgcg acatttttcg cgtcgatcag cacgcattcg
     2941 gatttgtcgg tttcaaggtc agcgatgaag gagacgagat agccgtcgtc ctcgtccttg
     3001 gcgtcgatgc gcggcgcgaa aggcgcttcg ctgccgaagc ggccggggcc gaactcatat
     3061 tcctcgctcg tgcccgcact caggtcgtgc ttgacgaggc cgcggaacag gaaccagccg
     3121 ggctcgggga tcgcgctgta tgcgtagcga tagggcttgc ccgcgtagcg atggttgaac
     3181 atgccgaact cgaggtcgcg gtcgtcgagc cgctcttccg tcgtctcgcc ggtcttgagg
     3241 ttgaagcgcc agcggtggag gcgcggcttg agcaatccct ggtcgagata ggccatcatc
     3301 cgctcgagcc catcgggcgc gttcggatag gatttgggca tcggctcttc ctgataatag
     3361 ccgtcgagga tgatctcgtc gccttcctcc cacgcattga gccagtggag cgtgtaggtc
     3421 ggctcggcct cgaaccagcg gatatcctca gggttcccgt ggcgcgggat gatcgcgaag
     3481 cgtgtctttt cgtcggggtg gaactggacg acgtgcaggt tccgttcgag gagttcctcg
     3541 ttccagtaga gcggcatgtc gttgaggatc gtgtaattct tcgtgaacgc catgtcgtgc
     3601 ggtaggcgcg gcccggcgag cgggatcggg atataatgct tcagcctgtt gtccgccccg
     3661 acgacgccat agtgcatgta gggcgcgtgc ttcgagtagt tgaagaacat cagctcgccg
     3721 gtcgcgaggt cgaccttgca atgcgccgaa atgccgtcga gcggcaccca gctttcggtg
     3781 ccatattgtt cgagcgtgaa ggggtcgagc cgataggctt cgccgcactg gtagaaggtc
     3841 gagatgatct tgcccgcgtg gatcgcaacg tcggtcgagg acgaatcctt gagccactcc
     3901 tgcgcgcccc agccgtggcg cgtcgatttg tgcggcggct ccatcagccc ggcccacagc
     3961 gagcgcccgg cttccttctc ggcctcgaac cccttggtgc gcacgaaacg gctgcgatag
     4021 ctcgcgcggc cgtccttgaa cgaaatcgaa tggatgaacc cgtcgccgtc gaaagggtga
     4081 tagcggccga tcggctcatg aatctggttc tcgccggtgc gcacatagac accctcgatg
     4141 tcggtcggga tattgccgat cacctcggca tcgccgtcgg cgaagatcgc gttccattca
     4201 ttataggtcg gacgccacgc gcccttcata taggggtggt cgttcggctg gatcgtagtg
     4261 gtgattgtgt cgacgagttc ggcggtcatg ctgggactcc ttcggcgggt gcaaagcgcg
     4321 gccttgcggc ggcggcatca tattctttca caaggcggtc gacgatatcc gcgaccggtt
     4381 cgacgccgcg caccgcgccg acgccttgcc cggcggacca gacattcttc cacgccttcg
     4441 catcgtcctg cgcatcggag aaattagggc gcttgtcctc gggcatgttc atcggatcat
     4501 agcccgcggc gatcaggctc gacttcagcc agttggcggt cacgccggtg atgcccttcg
     4561 aagggatgat atcctccgcg cccgcggcga cgatcatctc cttgtacccc ggcaccgcca
     4621 tgctttcggc gcaggcgatc agcgaagtgc cgagataggc gaaatcggcg cccaatatct
     4681 ccgcggcgcg gatcgcgtgg ccggtcgaga tcgccccacc cagcacgagc ggcccgtccc
     4741 agaattgccg cacctcttcg acgaaggcga aacccgcggt cgcgccggta t
//
LOCUS       DATMSU010000481         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_149883,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000481 DATMSU010000000
VERSION     DATMSU010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149883"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..2523
                     /locus_tag="VN034_12795"
     CDS             370..2523
                     /locus_tag="VN034_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWV61533.1"
                     /translation="MQQDLSPGTSQPPADTGLAAFAQILAMHRVVADPAELRHELGHS
                     DPVTASDLVRLAKRIEGLRARATTADFERLRRLPMPVLASGADGWFVIGRVGEDAVAV
                     QRPGAEIERWDRGALEARWSGALLLVATRDSAQVAAPAFDMTWFVPQIVKYRKLIGEV
                     LLITLALNLLGLAAPLFFQNVVDKVLAHNSLATLQVLAIGLVIVSFWEVAFGWLRTRL
                     YSETSQKLDVELGSRLFRHLLRLPLAYFENRRVGDTVTRVRELETVREFMTNASLTVL
                     VDPLFTIVFMVAMWLYSPLLFVIVALTLPAYVIVSLVVTGPLRRRLDEKFARGAANNA
                     LLVESVSGMQTLKAAAVEPQWQDRWERQLAAYSAANQRVINLGNSGSQAVQLISKLSL
                     AAILFFGAQQVIAGALTIGGLVAFNMFAQRVSGPVIRMAQLWQDFQQVRISVARMGDI
                     LNAGAEPGAGSRTALPALAGHVRFEAVRFRYAADGPWTLDDISLDLPAGATLGIAGAS
                     GSGKSTLTRLLQRLYLPASGRVLIDGVDIAQVDPAWLRRQIGVVLQENILFNRSIREN
                     IALADPVTPIERVMTAARLAGAHDFILELPRGYDTIVEERGVNFSGGQRQRLAIARAL
                     ITNPRILIFDEATSALDAESEEIVQRNLKAIAAGRTVLVIAHRLSAIRQCDRILTLDK
                     GRIVESGTHDELLRLGGRYAALHHRQIGVQAGAAA"
     gene            2520..>3010
                     /locus_tag="VN034_12800"
     CDS             2520..>3010
                     /locus_tag="VN034_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010188763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HWV61534.1"
                     /translation="MNALTRHWTAVRDALDNERARSRGVLRTEEADFLPAALEVVERP
                     VSPTARVTTWLLLGLLAAALLWLILGRVDVVASAPGKLAPADDVKLIQPGAAGIVRAI
                     LVRDGQTVRAGQPLVELDPTVSDADAAQARKTLETAELDAARLRAVLSALDGQGLRFT
                     PPAG"
BASE COUNT          437 a         1120 c          953 g          500 t
ORIGIN      
        1 tacctcgccg cccggcccgg ccgccgcgat cagctgcggc aaatcctcca gcgtcgcggc
       61 gttcgccgcg ctcgagccgt cgcccgaacc gctgccggtt ggcgagatgt agagggtggg
      121 catggcaggg ctcccgtcat aagcctgaaa acataccccc cgcttgatcg cgactgacaa
      181 cccgcgacgg catccgggtc aaccacatgc tgcgcctgcg aaagccgaat cgcccctttg
      241 ctccgacgac cggaggctcg gcgccttcgc tctccgggcc tgggccgcta ccaaggttcg
      301 ggccggacga ttcagcttgc cgcaccccgt agattgtcaa cgcctcaaga atccgatcgg
      361 agacccaccg tgcagcagga cctgtccccc gggacgtcgc agccgcccgc ggacaccggg
      421 ctcgccgctt tcgcccagat cctcgcgatg caccgcgttg ttgccgaccc ggcagagttg
      481 cgtcacgaac tgggccattc cgaccccgtc acggcatccg atctggtgcg cctcgcaaag
      541 cgcatcgagg gcctccgcgc tcgagcgacg accgccgatt tcgaacggtt aaggcgcctt
      601 cccatgccgg tgctggccag cggcgccgat ggctggttcg tgatcggccg cgtcggcgag
      661 gacgccgtgg ccgtccagcg ccccggcgcc gagatcgagc gctgggatcg cggcgcattg
      721 gaggcccgct ggtcgggcgc gctgctcctc gtcgccacgc gcgattcggc gcaggttgcc
      781 gcgcccgcct tcgacatgac ctggttcgtt ccccagatcg tcaaataccg gaagctgatc
      841 ggcgaggtgc tgctgatcac ccttgcgctc aatctgctcg gcctcgccgc gccgctcttc
      901 ttccagaatg tcgtcgacaa ggtgctggcg cacaacagcc tcgccacgct gcaagtgctc
      961 gccatcggcc tggtaatcgt ctcgttctgg gaagtcgcgt tcggctggct gcgcacccgg
     1021 ctctattccg agaccagcca gaaactcgac gtcgagctgg gcagccggct cttccgccac
     1081 ctgctccgcc tgccgctcgc ctatttcgag aatcgccgcg tcggcgatac cgtgacccgc
     1141 gtccgcgagt tggagacggt gcgcgagttc atgaccaatg cctcactcac cgtgctggtc
     1201 gatccgctct tcacgatcgt cttcatggtc gcgatgtggc tctattcgcc gctcctcttc
     1261 gtcatcgtcg cgctcacgct ccccgcctat gtgatcgtct cgctcgtcgt caccggcccc
     1321 ttgcgccgcc ggctcgacga gaagttcgcg cgcggcgccg ccaacaacgc gctgctcgtc
     1381 gagagcgtgt cgggcatgca gacgctcaag gccgccgcgg tcgagccgca atggcaggac
     1441 cgctgggaac gccagctcgc cgcctatagc gccgccaacc agcgcgtgat caacctcggc
     1501 aattcgggca gccaggcggt ccagctcatc tccaagctca gtctcgccgc gatattgttc
     1561 ttcggcgcgc agcaggtgat cgcgggcgcg ctgacgatcg gcgggctcgt cgcgttcaac
     1621 atgttcgcgc agcgcgtctc cggcccggtc atccgcatgg cgcagctctg gcaggacttc
     1681 cagcaggtcc gtatctcggt cgcgcggatg ggcgacatcc tcaacgcggg cgcggagccc
     1741 ggggcgggat cacgcaccgc gctgcccgcg ctcgcgggcc atgtccgctt cgaggcggtc
     1801 cgcttccgct acgccgccga cggcccctgg acgctcgacg acatcagtct cgacctgccg
     1861 gccggcgcca cgctcggcat cgcgggcgcc tcgggctcgg gcaagtcgac gctcaccaga
     1921 ttgcttcaac gcctatatct gcccgcaagc ggccgcgtgc tgatcgacgg agtcgacatc
     1981 gcgcaggtcg accccgcctg gctgcgccgc cagatcgggg tggtgttgca ggagaatatc
     2041 ctgttcaacc gctcgatccg agagaatatc gcgcttgccg atcccgtcac gccgatcgaa
     2101 cgggtgatga ccgccgcccg tctcgcgggc gcgcacgact tcatcctgga actgccccgg
     2161 ggctatgaca cgatcgtcga ggagcgcggc gtcaacttct cgggcggcca acgccagcgc
     2221 ctcgcgatcg cccgcgcgct gatcaccaac ccgcgcatcc tgatcttcga cgaggcgacg
     2281 tcggcgctcg atgccgagag cgaggagatc gtccagcgca acctcaaggc gatcgccgcc
     2341 ggccggaccg tgctggtcat cgcgcatcgc ctctccgcga tccgccagtg cgaccgcatc
     2401 ctgacgctcg acaaggggcg catcgtcgaa agcggcacgc atgacgagtt gctccgcctc
     2461 ggcggccgct atgctgcgct ccaccatcgc cagatcggcg ttcaggcagg ggctgcggca
     2521 tgaacgcgct cacccgtcac tggaccgcgg ttcgcgacgc gctcgacaat gagcgtgcgc
     2581 ggtcgcgcgg ggttctgcgc accgaggagg ccgacttcct tcccgccgcg ctcgaagtgg
     2641 tcgagcgccc tgtgtcgccg accgcgcggg tcaccacctg gctattgctc ggcctgctcg
     2701 cagccgcgct gctctggctg atccttggcc gggtcgacgt cgtcgcgtcg gcgccgggca
     2761 agctggctcc ggccgacgac gtcaagctga tccagcccgg cgcggcgggg atcgtccgcg
     2821 ccatattggt ccgcgacggt cagacggtcc gtgccggcca gccgctggtc gagctcgatc
     2881 ccaccgtctc ggatgccgac gcggcgcaag cccgcaagac gctcgagacc gccgaactcg
     2941 acgccgcacg gctacgcgcc gtcctttccg cgctcgacgg ccaaggcctg cgcttcaccc
     3001 cacccgccgg
//
LOCUS       DATMSU010000482         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_102764,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000482 DATMSU010000000
VERSION     DATMSU010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102764"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2626
                     /locus_tag="VN034_12805"
     CDS             <1..2626
                     /locus_tag="VN034_12805"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HWV61535.1"
                     /translation="KGALEAGRAEIERRLLERPSSGRVAANATAFLIDQIVRLSHDFT
                     THHLYPSANRSAGERITLIAVGGYGRGEMAPHSDIDIGFLTPFKQTSWTEQVIEAQLY
                     TLWDLGLKVGHSSRSLDEMVRAAKEDLTVRTALLEGRFIWGDRDLYDQASARFDAEVV
                     AGNARAFVADKLAERDERHKRMGDSRYVVEPNVKEGKGGLRDLHTLFWIGKFIHRVRT
                     VPELVDAGLMSARELRQFERAENFLLAVRCHLHILAGRAEDRLTFDFQREIASRMKFA
                     DRPGKSAVERFMQLYFLHAKSVGDVTGTFLAHLDDQMAARGRRFLPTIRRRPGKLHGF
                     VLDRGRLALPAEDFFAKDPVRLVEIFALADKHGLEIHPQAMRQARHDAKLIETQSVRR
                     DPRANELFLDVLTSPRDPETVLRWMNEAGVFGRFVPDFGRVVAQMQFDMYHHYTVDEH
                     TIRAIGLLADIEQDRLKDDHPLSTAIMDQIHSRRVIYVAVLLHDIAKGRGGDHSVLGA
                     ELALRVCPRLGLNDAETETVSWLVRYHLLMSATAFKRDLADFKTILDFAQIVQSAERL
                     RLLLVLTVVDIRAVGPGVWNGWKRQLLTELFDAAEEVLRLGHKQKGREARIESKKEAV
                     AAQFGFDAGTFEKLARRLPESYWIAEPVEVIAANMLHIRQAGDAPLHIAAVPDDDRGA
                     TLVMVLAADHPGLFYRIAGGIHLAGGNIIDARIHTTRDGLALDNFLVQDPLGRPFAEA
                     GQIGRLTRAIEDALANRQKLLPKLEARALPRTRAEAFRIAPSVFVDNKASNRFTVIEV
                     NAQDRPALLNQLAYALFQSKVTVHSAHVATYGERAVDTFYVTDLIGDKIDSAARVKSL
                     EKRLLEAAGSRSEEAVAA"
     gene            complement(2642..>3042)
                     /locus_tag="VN034_12810"
     CDS             complement(2642..>3042)
                     /locus_tag="VN034_12810"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWV61536.1"
                     /translation="RSTPPAGIAAVIDAATRYFAGERIEFFTVAVDPGDQPPFFARVY
                     EHVRTLGWGETTTYGAVARALDAGPEQARAVGQAMATNPVPLIIPCHRVMAAGGGIGG
                     FSAPGGSQSKARMLELEGVPLPPVQQGFDF"
BASE COUNT          520 a         1044 c          991 g          487 t
ORIGIN      
        1 caagggcgcg ctcgaagcgg gccgcgccga gatcgagcgc cggctgctcg aacgcccctc
       61 gtcggggcgc gtcgcggcga atgcgaccgc cttcctcatc gaccagatcg tccgcctcag
      121 ccacgatttt accacccacc atctctatcc ctcggccaat cgctcggcgg gtgagcgcat
      181 cacgctgatc gcggtcggcg gctatggccg cggcgaaatg gcgccgcaca gcgacatcga
      241 catcggcttt ttgaccccgt tcaaacagac gagctggacc gaacaggtga tcgaggcgca
      301 gctttatacg ctctgggacc tcggactgaa ggtcggccat tcgtcgcgtt cattggacga
      361 gatggtgcgc gcggcgaagg aggatctcac cgtccgcacc gcgctgctcg aagggcgctt
      421 catctggggc gaccgcgacc tttacgacca ggcctcggcg cgcttcgatg ccgaggtcgt
      481 cgcgggcaac gcgcgcgcct tcgtcgccga caagctcgcc gagcgcgacg agcggcacaa
      541 gcgcatgggc gattcgcgct atgtcgtcga acccaatgtg aaggaaggga agggggggct
      601 gcgcgacctc catacgctat tctggatcgg caagttcatt caccgcgtcc gcaccgtgcc
      661 cgagctcgtc gatgcggggc tgatgtcggc gcgcgagctg cgccagttcg agcgcgccga
      721 aaatttcctg ctcgcggtgc gctgccatct ccacatcctc gctgggcgcg ccgaggaccg
      781 gctgaccttc gatttccagc gcgagatagc gagccgcatg aaattcgccg accggccggg
      841 caagagcgcg gtcgaacgct tcatgcagct ctatttcctc cacgccaaaa gcgtcggcga
      901 cgtcaccggc accttcctcg cgcatctcga cgaccagatg gcggcgcggg ggcggcgctt
      961 cctgccgacg atccggcggc ggccgggcaa gctccacggc ttcgtcctcg accgcgggcg
     1021 gctcgcgctg ccggcggagg atttcttcgc gaaggatccg gtgcggctcg tcgagatttt
     1081 cgcgctcgcc gacaagcacg gcctcgaaat ccatccgcag gcgatgcggc aagcgcgcca
     1141 cgacgccaag ctgatcgaga cgcagagcgt gcgccgcgac ccccgcgcta acgagctgtt
     1201 cctcgacgtg ctgacgtccc cgcgcgatcc cgaaacggtg ctgcgctgga tgaacgaggc
     1261 gggggtgttc ggccgcttcg ttcccgattt cggccgcgtc gtcgcgcaga tgcagttcga
     1321 tatgtatcat cattatacgg tcgatgagca tacgatccgc gcgatcgggc tgctcgccga
     1381 catcgagcag gaccggctca aggacgatca cccgctgtcg accgcgatca tggaccagat
     1441 ccactcgcgc cgcgtcatct atgtcgcggt gctgctccac gacatcgcca agggccgcgg
     1501 cggcgaccat agcgtcctcg gcgccgagct ggcgctgcgg gtctgcccgc ggctcgggct
     1561 gaacgatgcc gaaaccgaga cggtgtcgtg gctcgtgcgc tatcatctcc tgatgtcggc
     1621 gaccgcgttc aagcgcgacc tcgccgactt caagacgatc ctcgactttg cgcagatcgt
     1681 gcagagtgcc gaacgcctgc gcctgctcct cgtgctcacc gtcgtcgata tccgcgcggt
     1741 cgggccgggc gtgtggaacg gctggaagcg ccagctgctc accgagctct tcgacgccgc
     1801 cgaagaagtc ctgcgcctgg ggcacaagca gaaagggcgc gaggcgcgga tcgaaagcaa
     1861 gaaggaggcc gtggccgccc aattcggctt cgatgccggg acgtttgaaa agctcgcgag
     1921 gcggcttccc gaaagctact ggattgccga gccggtcgaa gtgatcgcgg cgaatatgct
     1981 ccatatccgc caggcgggcg atgcgccgct ccatatcgcc gcggtgcccg acgacgatcg
     2041 cggggcgacg ctggtgatgg tgctcgccgc cgaccatccg gggcttttct atcgcatcgc
     2101 cggcggcatc cacctcgccg gcggcaatat catcgacgcg cgcatccaca cgacgcgcga
     2161 cggcctcgcg ctcgacaact tcctcgttca ggaccccctc ggccgtccct ttgccgaggc
     2221 gggccagatc ggccggctga cgcgcgcgat cgaggacgcg ctcgccaacc gccagaaact
     2281 gctccccaaa ctcgaagcgc gcgcgctgcc gcgcacccgc gccgaggcct tccgcatcgc
     2341 ccccagcgtc ttcgtcgaca acaaggcgtc gaaccggttt acggtgatcg aggtcaatgc
     2401 gcaggatcgc cccgcgctat tgaaccagct tgcctatgcg ctcttccaat cgaaggtcac
     2461 ggtgcacagc gcgcatgtcg ccacctatgg cgagcgcgcg gtcgatacat tttacgtcac
     2521 cgacctgatc ggcgacaaga tcgacagcgc ggcgcgggtg aagagcctcg aaaagcgcct
     2581 gctcgaagcc gccggcagcc gcagcgagga agccgtcgcg gcctagagtc ccggaaagac
     2641 cctaaaagtc gaacccctgt tggaccggag gaagcggcac gccttcgagt tcgagcatcc
     2701 gcgccttcga ttgcgagccg cccggcgcgg agaacccgcc gattccgccg cccgcggcca
     2761 tgacgcgatg gcaggggatg atcagcggca cgggattggt cgccatcgct tgcccgaccg
     2821 cgcgggcctg ttcgggaccg gcatcgagcg cacgcgcgac cgcgccatag gtcgtcgtct
     2881 cgccccagcc gagcgtgcgc acatgttcat agacgcgcgc gaagaagggc ggctggtcgc
     2941 ccggatcgac ggccaccgtg aaaaattcga tgcgttcgcc ggcaaaatag cgggtcgcgg
     3001 cgtcgatgac tgccgcgatt ccggcgggcg gcgtgctgcg at
//
LOCUS       DATMSU010000483         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_149887,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000483 DATMSU010000000
VERSION     DATMSU010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149887"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VN034_12815"
     CDS             <1..1144
                     /locus_tag="VN034_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HWV61537.1"
                     /translation="VADLPEPAAPAPDPSLIEWADVSDASACQLEIGYALVTLVDERK
                     GSPLMTRITGIRRQLSKELGFVVPPVKVSDDLSLPGNVYRISVAGVIVGEDEVFPNEM
                     LALDSGDLVTKVTGRPCKDPTFGLDALWIPKAMQNDAIAAGYTVVDPATVVATHLNNS
                     IVGSAAELFGIDDAQALIDNLKTHYPQLAQNLSPQGYSLPRVASLCKSLLVERVPLRD
                     FRKIAEAMVALSAQQLGEIDLIEAVRQRIGALIVQTIVPSRMPLPVVTFSPEIEMLLN
                     QAVRANPAAEWPFEHGMAMTIIEQVGQAVEPLLLQTRSFALVASPICRSALSRLVRAT
                     FPDVAVISYLEIPATKQTEIVATIGAEVPRLASGPGAPMEDQVHEN"
     gene            1134..1877
                     /locus_tag="VN034_12820"
     CDS             1134..1877
                     /locus_tag="VN034_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HWV61538.1"
                     /translation="MRIEPAEQFAASGRAPGTRGYGESVEALVAEHAPLVRKIAWQVF
                     SRVSRTSELDDLIQTGLIALIEASRHYEERGFAFATYATTRIRGAMIDQLRREADVGR
                     SAMVAAKRIQAMRGALEQQLMRAPTAAEMAVAFDMSAEDYFALERNATHGRSTSLDEL
                     TDVGAFLAADEDAGADERCEQEDLMGALRQCVGRLSEREQMVLQLYFFEELNLHEIGL
                     TLDVSAARICQIKREAMIKVDRMMKEMTE"
BASE COUNT          359 a          720 c          680 g          334 t
ORIGIN      
        1 ggtcgccgac ctgcccgaac ccgccgcccc cgcgcccgac ccgtcgctga tcgaatgggc
       61 cgacgtcagc gacgcgagcg catgccagct cgagatcggc tatgcgctcg tcaccctcgt
      121 cgacgaacgc aagggatcgc cgctgatgac gcgcatcacc ggcatccgcc gccaattgtc
      181 gaaggaactc ggcttcgtcg ttccgccggt gaaggtcagc gacgacctgt cgctgccggg
      241 caacgtctat cgcatctcgg tcgccggcgt gatcgtcggt gaagatgaag tctttcccaa
      301 cgagatgctc gcgctcgatt cgggcgacct cgtcaccaag gtgaccgggc ggccgtgcaa
      361 ggatccgacc ttcggcctcg atgcgctgtg gattccgaag gcgatgcaga atgacgcgat
      421 cgcagcgggc tataccgtcg tcgaccccgc caccgtcgtc gcgacgcacc tcaacaacag
      481 catcgtcgga tcggccgccg agctgttcgg catcgacgat gcgcaggcgc tgatcgacaa
      541 tctcaagacg cattatccgc agctcgcgca gaatctcagc ccgcagggct attcgctgcc
      601 gcgcgtcgcg agcctctgca aatctttgct cgtcgagcgc gtgccgctgc gcgatttccg
      661 caagatcgcc gaagcgatgg tcgcgctgtc ggcgcagcaa ctcggcgaga tcgacctcat
      721 tgaagcggtg cgccagcgca tcggcgcgct gatcgtccag acgatcgtgc cgagccggat
      781 gccgctgccc gtcgtcacct tcagccccga gatcgagatg ctgctcaacc aggcggtgcg
      841 cgccaacccc gccgccgaat ggccgttcga acatggcatg gcgatgacga tcatcgaaca
      901 ggtcgggcag gcggtcgagc cgctgctgct ccagacgcgc agcttcgcgc tcgtcgcctc
      961 cccgatctgc cgctccgccc tctcgcgcct cgtgcgcgcc actttccccg acgtcgccgt
     1021 catctcctac ctcgagattc cggcgaccaa gcagaccgaa atcgtcgcga cgatcggcgc
     1081 cgaagtgccg cgcctcgcgt ccgggcccgg cgcccccatg gaggaccaag tgcatgagaa
     1141 ttgagcccgc cgagcaattt gccgcgagcg gccgagcgcc gggtacacgc ggctatggcg
     1201 aaagcgtcga ggcgctggtc gcggaacatg cgccgctggt gcgcaagatc gcgtggcagg
     1261 tcttttcgcg cgtgtcgcgg acgagcgagc tcgacgacct gatccagacc gggctgatcg
     1321 cgctgatcga agcgagccgc cactatgagg agcgcggctt cgccttcgcc acctatgcga
     1381 cgacgcgcat ccgcggcgcg atgatcgacc agctacgtcg cgaggccgat gtcggccgct
     1441 cggcgatggt cgccgcgaag cggatacagg cgatgcgcgg cgcgctcgaa cagcagctga
     1501 tgcgcgcgcc gacggccgcc gagatggcgg tcgccttcga catgtcggcc gaggattatt
     1561 tcgcgctcga acgcaatgcg acgcacggcc gctcgacctc actcgacgag ctgaccgacg
     1621 tcggcgcctt cctcgccgcc gacgaggatg ccggtgccga cgaacgctgc gagcaggaag
     1681 atctgatggg ggcgctgcgc cagtgcgtcg gccgcttgtc cgagcgcgag cagatggtgc
     1741 tgcagctcta tttcttcgaa gagctcaacc tgcacgaaat cggcctgacg ctcgacgtca
     1801 gcgcggcgcg catctgccag atcaagcgcg aggcgatgat caaggtcgac cgcatgatga
     1861 aggaaatgac cgaataggag ggaccgggtg cggtgcgcgc gaccagtcgc cctcgcaccc
     1921 gatcctgggg ggttaaaccc agagctctag gccgccagcg ggggaagagc ctggcggacg
     1981 ccgccgatct tttcgaaata gctcgccatg cgttcggtcc cgttttcgct caggcgcacg
     2041 atcgtgcggc ggcgatcgcg ctcgtcgatc gagcggtgca ccagcccgcg ctg
//
LOCUS       DATMSU010000484         3118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_122118,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000484 DATMSU010000000
VERSION     DATMSU010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122118"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VN034_12825"
     CDS             <1..421
                     /locus_tag="VN034_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HWV61539.1"
                     /translation="TGTLVDIFRADPHASLLGTDALRDGVDVPGDSLRLVVMEGVPWP
                     RPTILHAARRAAQGGSAYDDMVIRARIGQAFGRLIRRADDFGQFVMLSPSFPSRLLSA
                     FPEGTPIRRLPLDEAVNHVAAANVERRKSAFPAFSPS"
     gene            471..1022
                     /locus_tag="VN034_12830"
     CDS             471..1022
                     /locus_tag="VN034_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWV61540.1"
                     /translation="MKTLTILRHAKSGWDVAVERDFDRPINKRGQRGAELIGQWLKRH
                     KLPVDRIIASPAVRVTETLDIFQPAAGLDGLEPHWDRRIYLASAATLIDVIRDTGRDA
                     QHLLISGHNPGLEDLILMLVPESDDDELRARVEEKLPTSALARLDLDIADWHGLDVNI
                     ARFADFIRPRDLDPALAPAMDDD"
     gene            complement(1198..1800)
                     /gene="folE"
                     /locus_tag="VN034_12835"
     CDS             complement(1198..1800)
                     /gene="folE"
                     /locus_tag="VN034_12835"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWV61541.1"
                     /translation="MSDNGQELGPDGKVVVPDHVADAVRTLIEWAGDDPAREGLLDTP
                     RRVARAWKEYCSGYADDPALYLSRTFEEVGGYDEIVLLRDIPFQSHCEHHMAPITGKA
                     SIAYLPRDRVVGISKLARVLNGFSRRLQVQERLTAEVAKCIWDNLQPHGVAVVIDAQH
                     GCMTGRGVRTPGVGMVTSRLLGCFLDDERSRKEVLSLMGY"
     gene            2235..>3118
                     /locus_tag="VN034_12840"
     CDS             2235..>3118
                     /locus_tag="VN034_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWV61542.1"
                     /translation="MKFKALIGTSILAMTVATPAYAQDAADEVERDAFGGEIVVTAQR
                     QSERLQDVPIAVSAFSTEALEAQQIKTPSDLQLTLPNVTFTKTNFTGASFTIRGIGDL
                     CVGTTCDSATAIHLNGDPLFSTRLFETEFFDLERVEVLRGPQGTLFGRNATSGVVNVI
                     TAKPKLGVFEAAAEAEYGNYNSMKGKAMVNIPLGDTMGIRVAGIYLNRDGYTKNSFLD
                     TRIDDRDLYSVRGSFRWEPSPDTTIDLLASYFREKDKRTRIQKQLCQRDPTGILGCLN
                     SRLDNSPFNGNATFTAALT"
BASE COUNT          587 a         1064 c          912 g          555 t
ORIGIN      
        1 caccggcacg ctcgtcgaca ttttccgcgc cgatccgcac gcctcgctgc tgggtaccga
       61 tgccttgcgc gacggggtcg acgtgcccgg cgattcgctg cggctagtcg tgatggaagg
      121 cgtgccctgg ccgcgcccga cgatcctcca cgccgcgcgc cgcgctgcgc agggtggcag
      181 cgcctatgac gatatggtca tccgcgcgcg catcgggcag gcgttcggcc gcctgatccg
      241 ccgcgccgac gatttcggcc agttcgtgat gctctcgccc agctttccct cgcggctgct
      301 cagcgctttc cccgaaggca ccccgatccg ccgcctgccg ctcgacgagg cggtcaacca
      361 tgtcgccgcg gcgaatgtcg aacgcagaaa aagcgccttt ccagcctttt caccgtcata
      421 agtttgcggc acaaggcccg cgaacgaggg cgcgatcgcg gggagatatt ttgaagactt
      481 tgaccatcct gcgccacgcc aagtcgggtt gggacgtggc cgtcgagcgc gacttcgacc
      541 gtccaatcaa taaaaggggc caacgcggcg ccgaactgat cggccaatgg ctgaaacggc
      601 acaagctgcc cgtcgaccgc atcatcgcct cacccgcggt gcgcgtgacc gaaacgctcg
      661 acatcttcca gcccgccgcc ggcctcgacg gcctcgaacc gcattgggac cgccgcatct
      721 atctcgcctc ggccgcgacg ctgatcgacg tgatccgcga taccggccgc gatgcgcagc
      781 acctcctgat ttcgggccat aacccgggcc tcgaggatct gatcctgatg ctcgtccccg
      841 aatcggatga cgatgaactg cgcgccaggg tcgaggaaaa gctcccgacg tcggcgctgg
      901 cgcgcctcga cctcgatatc gccgactggc acggcctcga cgtgaacatc gcgcgcttcg
      961 ccgacttcat ccgcccccgc gacctcgacc ccgcgctcgc ccccgccatg gacgacgatt
     1021 aggctgtatc gacattcaga agatggcgag tcgaaaatgg tggttttccg tgacccggag
     1081 cgcagcgtgc gttctgcacg tgagcaccgg aagcgcggaa ggccgtcatt tgcagaccgc
     1141 cagggctgaa tgtcgataca gcctaagcca ggcgcgaccg gcaaacgcca gcctacctca
     1201 atagcccatc aggctcagca cttccttgcg gctgcgctcg tcgtcgagga agcagccgag
     1261 cagacggctc gtcaccatgc cgacacccgg cgtgcgcacg ccgcgcccgg tcatgcagcc
     1321 atgctgcgca tcgatcacca ccgcgacgcc gtggggctgg agattgtccc agatgcactt
     1381 cgcgacctcg gcggtcaggc gttcctgcac ctgcagccgc cgcgaaaagc cgttgagcac
     1441 gcgggcgagc ttcgaaatgc cgacgacgcg gtcgcgcggc agataggcga tcgacgcctt
     1501 gcccgtgatc ggcgccatat gatgttcgca atgcgactgg aacggaatgt cgcgcagcag
     1561 cacgatctcg tcatagccgc cgacttcctc gaaggtgcgc gacaggtaaa gcgccggatc
     1621 gtcggcataa cccgaacaat attccttcca cgcgcgcgcg acacggcgcg gtgtgtcgag
     1681 cagtccctcg cgcgccggat cgtcgcccgc ccactcgatc agcgtccgca cggcgtccgc
     1741 aacatggtcg ggaacgacca ccttgccgtc cggtcccaac tcttgtccgt tgtcgctcat
     1801 cgccccgctt tcactgttgc cttcatgtcg cacatttcgg ggcgaatgcg acgataagca
     1861 agggccagag aagaagaaat tgtcctctcc catcaacttt acgttgcgga atcttggctt
     1921 tttcactttt cggagaggcg cggtttcgcg tccttttttg aaccaaacaa gtcacttgac
     1981 gctttcgtca actcggcgca cctttgctcc atacagaact cgctcaagcg ggtcgtattc
     2041 cggagaggcc ggcgttttcc ccgacgccgg tgggagagga cgatgaccag cacagctggc
     2101 aagtgggatt cacgagggca catggccgct ttagccgggt gccctttgcc atgtctggtc
     2161 gtttcccctc cccttaccac aatttgacgt tgggcggccc cgcatcgcgc gggatcgaaa
     2221 aagggaggct atagatgaag ttcaaggcac tgatcggaac ctcgatcctt gcgatgacgg
     2281 tggcgacgcc ggcatatgcg caggacgcgg cggatgaagt ggaacgcgat gctttcggcg
     2341 gcgaaatcgt cgtcacggcg cagcgccagt cggaacgtct gcaggacgtg ccgatcgcgg
     2401 tcagcgcctt ttcgaccgag gcgctcgaag cgcagcagat caagacaccg tccgatcttc
     2461 agctgacctt gcccaacgtc actttcacca agaccaactt cacgggcgcc agcttcacga
     2521 tccgcgggat cggcgacctt tgcgtcggca cgacctgcga cagcgcaacc gcgatccatc
     2581 tgaacggcga cccgctgttt tcgacccgcc tgttcgaaac cgaattcttc gacctcgaac
     2641 gcgtcgaagt gctgcgcggg ccgcagggaa cgctgttcgg ccgcaacgcg acgtcgggcg
     2701 tggtcaacgt gatcaccgcc aagcccaaac tcggcgtctt cgaagccgcc gccgaagccg
     2761 aatatggaaa ttataattcc atgaagggca aggcgatggt gaacatcccg ctcggcgaca
     2821 cgatgggcat ccgcgttgca ggcatttacc tcaaccgcga cggctatacg aagaacagct
     2881 tcctcgacac ccgcatcgac gaccgcgatc tttattcggt ccgcggctcg ttccgctggg
     2941 agccctcgcc cgacacgacg atcgacctgc tcgcatccta tttccgcgaa aaagacaagc
     3001 gcacgcgcat ccagaaacag ctttgccagc gcgatccgac cggcatcctc ggctgtttga
     3061 acagccgtct cgacaattcg cccttcaacg gcaacgcgac cttcaccgcc gcgctgac
//
LOCUS       DATMSU010000485         3696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_159863,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000485 DATMSU010000000
VERSION     DATMSU010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159863"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VN034_12845"
     CDS             <1..996
                     /locus_tag="VN034_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWV61543.1"
                     /translation="MRRAATGGALVRTLALTASRRGQARIDALWIRWAGPLGLVWKQR
                     KFEIDGAIHIVPSLRAVTDEGSRLFQRNSWFGLRQQRFRGEGTEFESLAEYQPGMDRR
                     AIDWTASARHVKLLAKEYRVERDNRVVLAIDSGRTMAEPVGGMPRVDRAVSAALLLAY
                     VGLKLNDRISFFSFAAKPQTLTPAYMHTQDFPALQRAASLIDYAHVESNFTLALTTLS
                     AQLNRRSLIILFTEFTDATSADLMIRAAGRLVQKHRLLFVVIRDEEVESEERRRPETG
                     ADVTRSNVAAAMLRDRQLVIARLQRLGADVIEVPADAMGASAVEAYLGIKRQGSL"
     gene            993..2033
                     /locus_tag="VN034_12850"
     CDS             993..2033
                     /locus_tag="VN034_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HWV61544.1"
                     /translation="MIPPAAPTRSIDAPNFSTSRFRAEREADWIAFDLLLTKLEKKGA
                     KALTSDELLQLPLLYRATLSSLSIARATSLDKALLGHLEALSIRGYFLVYGVRESRWS
                     RLRRFFLYDWPAAVRSVWKETLIIALVILLGALTSYSLVSSNPEWYFNFVDEEMSGGR
                     DPRATAEFLQTTLGHGKAAAEKGESGLHIFATYLFTHNSRVSILSFALGFAFGVPTLM
                     LEYYQGIGLGAMMAVFSAKGLGFDFGGWLFIHGTTELFAAALSGAAGLRIGTAVVFPG
                     ARGRLQAASEAGRTAGKVMVGVILMLLAAGLLEGFGRQLITDTMLRYAIGSLMLLFWL
                     AYYYIPRREDVT"
     gene            2030..2896
                     /locus_tag="VN034_12855"
     CDS             2030..2896
                     /locus_tag="VN034_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWV61545.1"
                     /translation="MTAAANAKHRAASAKKIRQFVTPEGVDLELRIASAGLRLGALLV
                     DVVLILLALFLFSLFMLWVGLASRSDVTVVVWLLGSFVLRTFWFIGFELGSRAATPGK
                     RLMGIRVVARDGGRLTADAVVARNLIRELELFLPLMMLGSGAAEDMVSGWIILAGVLW
                     SLTLSLFLLFNRDRMRMGDLIAGTWVVMAQRVKLDADIAVDAAGGAMMFTEAELAVYG
                     IFELQELERVLRGRDPRAMREVADAIRAKIGRPVAEEDDVFLLSYYRQLKARLERKLL
                     FGKRREDKYASD"
     gene            2883..3569
                     /locus_tag="VN034_12860"
     CDS             2883..3569
                     /locus_tag="VN034_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HWV61546.1"
                     /translation="MPATDKSVTEALHKRRSVRAFTDRSVDPALLKEIFAAAQRAPSG
                     GNLQPWQVTVLTGEPWQAVKDAVAARIAMGREGHEPEYDIYPRALNEPWTDRRFGVGE
                     ALYASLGIPREDKAGRLARFMDNYRGFDAPVMLFLHCSRIMGPPQWSDMGMWLQSVML
                     LLVEHGLASCPQECWAMYGATIRRTLGLDEGQILFTGLAIGYADEDAAVNQWPVPRAP
                     LDEVIDWQGF"
BASE COUNT          553 a         1198 c         1271 g          674 t
ORIGIN      
        1 atgcgcaggg cggccacggg cggagcactc gttcgcacgc tcgcgctcac cgcatcgcgg
       61 cgcggacagg cgcgcatcga cgcgctgtgg atccgctggg cggggccgct tgggctcgtg
      121 tggaaacagc gcaagttcga aattgacggc gcgatccata tcgtcccgag cctgcgcgcg
      181 gtcaccgacg agggcagccg gctgttccag cgtaacagct ggttcggcct tcgccagcaa
      241 cgctttcgcg gcgagggcac cgagttcgaa tcgctcgccg aatatcagcc ggggatggac
      301 cggcgcgcga tcgactggac cgcgtcggcg cgccacgtca aactgctcgc caaggaatat
      361 cgcgtcgagc gcgacaaccg cgtcgtcctc gcgatcgaca gcgggcggac gatggccgag
      421 cccgtcggcg gcatgccgcg cgtcgaccgc gcggtgtcgg cggcgctgct gctcgcctat
      481 gtcgggctca aactcaacga ccggatcagc ttcttctcct tcgccgccaa gccgcagacg
      541 ctgacccccg cctatatgca cacgcaggat ttcccggcgc tgcagcgcgc cgcgagcctg
      601 atcgactatg cgcatgtcga gagcaatttc accctcgcgc tgacgacgct cagcgcgcag
      661 ctgaaccgcc gctcgctgat catccttttc accgaattca ccgacgcgac gagcgccgac
      721 ctgatgatcc gcgccgcggg gcggctcgtc cagaaacacc ggctgctgtt cgtcgtgatc
      781 cgcgacgagg aggtcgagag cgaggagcgg cgccggcccg agaccggtgc cgacgtcacg
      841 cgctcgaacg tcgccgcggc gatgctgcgc gaccgccagc tggtgatcgc gcggctccag
      901 cgtttgggtg ccgacgtgat cgaagttccc gccgacgcga tgggggcgag cgcggtcgag
      961 gcctatctcg gcatcaaaag gcagggcagc ctgtgatccc gccagcagcg cccacgcgaa
     1021 gcatcgacgc gccgaacttc tcgaccagcc gtttccgcgc cgagcgcgag gccgactgga
     1081 tcgccttcga ccttttgctg accaagctcg agaagaaggg cgcgaaggcg ctgaccagcg
     1141 acgagctgct ccagctcccg ctcctctacc gcgccaccct gtcttccttg tcgatcgcgc
     1201 gcgcgacgag cctcgacaaa gcgctgctgg gtcatctcga ggcgctgtcg atccgcggct
     1261 atttcctcgt ctatggcgtg cgcgaaagcc gctggagccg cctccgccgc ttcttcctct
     1321 acgactggcc cgccgcggtg cgctcggtgt ggaaggagac gctgatcatc gcgctggtca
     1381 tcctgctcgg cgcgttgacc agctattcgc tcgtatcgag caaccccgag tggtatttca
     1441 acttcgtcga tgaggagatg tcgggcggcc gcgacccgcg cgcgaccgcc gaatttctcc
     1501 aaacgacgct ggggcatggc aaggcggcgg cggagaaggg cgaaagcggg ctgcacatct
     1561 tcgccaccta tttgttcacg cacaacagcc gcgtatcgat tctgtccttc gcgctcggct
     1621 tcgccttcgg ggtgccgacg ctgatgctcg aatattatca ggggatcggg ctcggcgcga
     1681 tgatggcggt cttttccgcg aaggggctcg gtttcgattt cggcggctgg ctgttcatcc
     1741 acggcacgac cgaattattc gccgccgcct tgtcgggtgc cgcgggcctg cgcatcggca
     1801 cggcggtcgt cttccccggc gcgcgcggtc ggttgcaggc ggcgtcggag gcggggcgca
     1861 ccgcgggcaa ggtgatggtg ggcgtcatcc tgatgctgct ggccgcgggg ctgctcgaag
     1921 gcttcgggcg ccaactgatc accgacacga tgctgcgcta tgccatcggg tccctgatgc
     1981 tgctcttctg gcttgcctat tattatattc cgcggcgcga ggatgtgaca tgaccgccgc
     2041 cgccaacgcg aaacatcgcg ccgccagcgc gaagaagatt cgtcagttcg tgacgcccga
     2101 gggcgtcgat ctcgaactca ggatcgcgag cgcggggctg cgcctcggcg cgctgctcgt
     2161 cgatgtggtg ctgatcctgc tcgcgctgtt cctcttttcg ctgttcatgc tgtgggtcgg
     2221 cctcgcttcg cggtccgacg tcaccgtcgt cgtgtggctg ctcggttcct tcgtgctgcg
     2281 caccttctgg ttcatcggct tcgaactcgg ctcgcgtgcc gcgacgccgg gcaagcggct
     2341 gatgggcatt cgcgtcgtcg cgcgcgatgg cgggcgcctg accgccgatg cggtcgtggc
     2401 gcgcaacctg atccgcgagc tcgagctgtt cctgccgctg atgatgctgg gatccggcgc
     2461 ggcggaggat atggtttcgg ggtggatcat tctcgcgggc gtgctctggt cgctgacgct
     2521 cagcctgttc ctgctgttca accgcgaccg gatgcggatg ggcgatctga tcgcgggcac
     2581 gtgggtggtg atggcgcagc gggtcaagct cgacgccgat atcgcggtcg atgcggcagg
     2641 cggcgcgatg atgttcaccg aggccgagct cgcggtctac ggcattttcg agctgcagga
     2701 actcgagcgg gttttacgcg gccgcgatcc gcgtgcgatg cgcgaagtcg ccgatgcgat
     2761 ccgcgccaag atcgggcgtc ccgtcgccga agaggatgac gtcttcctgc tttcctatta
     2821 ccgccagttg aaagcccggc tcgaacgcaa gctgctgttc ggcaagcggc gagaggataa
     2881 atatgccagc gactgacaaa agcgttaccg aagctctcca caaacgccgc tcggtgcggg
     2941 cctttaccga ccggtcggtc gatccggcgc tgctcaagga gattttcgcg gcggcgcagc
     3001 gcgcgccctc gggcggcaat ctgcagccgt ggcaggtgac ggtgctgacc ggcgaaccct
     3061 ggcaggcggt gaaggatgcg gtggcggcgc gcatcgcgat ggggcgcgag gggcacgagc
     3121 ccgagtatga catctatccc cgcgcgctga acgagccctg gaccgatcgc cgcttcgggg
     3181 tcggcgaggc gctctacgcc tcgctcggca tcccgcgcga ggacaaggcg gggcggctcg
     3241 ctcggttcat ggacaattac cggggtttcg acgcaccggt gatgcttttc ctccattgct
     3301 cgcgcatcat ggggccgccg caatggtcgg acatggggat gtggctgcag tcggtgatgc
     3361 tgctgctcgt cgaacatggc ctcgcgagct gtccgcagga atgctgggcg atgtatggcg
     3421 cgacgatccg ccgcacgctc gggctggacg aggggcagat tttgttcact gggctcgcga
     3481 tcggctatgc ggacgaagat gcggcggtga accagtggcc ggtgccgcgc gcgccgctgg
     3541 acgaagtgat cgattggcag gggttttaag gatgaaaatg tccgggtttc gttatgcggt
     3601 ggcgggcacg ctgatgctgg tggtcgcggg ctgcgccgcg attccccagc ctgaggctcc
     3661 gccgcccgca ccgcggcccg tcgcgcccgc accgac
//
LOCUS       DATMSU010000486        11900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_93757, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000486 DATMSU010000000
VERSION     DATMSU010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11900
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93757"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VN034_12865"
     CDS             <1..864
                     /locus_tag="VN034_12865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV61547.1"
                     /translation="AWRTSLRLPGGAEARLTLDDLLAQRTGLTRHAYDEKLEEGRDPA
                     LLRASLGAAPLQCEPGTCYTYQNVAFDAASEILGEAANEIFADVVENRFFRPLGMISA
                     GYGMARLTGAKDWAKPHRGAHVHPVKEAYWRVPAAAGVESDIVDFATWMQAMMGSRPD
                     VLPAPALQLAHRPRVGTGRLYGGPLRQATSDASYGLGWRSFTYGGRRLEGHSGAVEGY
                     RATMIFDPATRTGVVAMWNSDWGFPFRIPFAAIDSYHQREDTNWLDLGELPPVTRART
                     AAKAIVEPPKG"
     gene            933..1910
                     /locus_tag="VN034_12870"
     CDS             933..1910
                     /locus_tag="VN034_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61548.1"
                     /translation="MRVLLTGSSGWLGRYLAPLLAKNGHKVVGLDVVSGTYTEVIGTV
                     ADRALVERTMGEYGIEAVIHGGALHKPDIIRYPRQAFIDVNVTGTLNLIEAAIAAGND
                     RFLFTSTTSLMVRADVRAGAGEAGAWWMDEDFGPIEPRNIYGITKLAAEQACKLVHRE
                     HGINVAILRTGRFFPEDDDTHAIPSGPNLKANELLNRRLTVEDAAAAHLAALEAAPAI
                     GCDTFILSAPPPFAREEAEELARDARAVIARHHPDAAELYAAEGWILPESIDRVYDPS
                     RAERRFGWRAKADFGSLLAALRDRTPLPFAHDPDYTSPIIAQERDACMC"
     gene            1898..2578
                     /locus_tag="VN034_12875"
     CDS             1898..2578
                     /locus_tag="VN034_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV61549.1"
                     /translation="MYVLITANRNYSSWSLRPWMLMQGLAIPFEDQIEPFTNPVNYDE
                     FRSFSPTGQVPALLDEGRTVYDSLGITLYLADRHDGVWPADPDARAWAQCAVAEMHSG
                     FSALRNDCTMNVGVRVTPKPPSGALRLDIARIRELFAEGLSRFGGPYLAGDRFTAADA
                     FYAPVAFRIRTYGLDVGAGQAWVDHMLTHPAMQDWERQALAESWREESHEAELMAAGA
                     ITADYRTA"
     gene            complement(2696..3214)
                     /locus_tag="VN034_12880"
     CDS             complement(2696..3214)
                     /locus_tag="VN034_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWV61550.1"
                     /translation="MSTPAILALILVTVVAMEFVAWASHKYIMHGFGWAWHRDHHEPH
                     GHVLEKNDLFALVGAAMSISMFMAGSPMILGASAWEPGTWIGLGILLYGIIYTLVHDG
                     LVHQRYFRWVPRRGYAKRLVQAHKLHHATLGKEGGVSFGFVFARDPAKLKAELKAQRE
                     AGIAVVREALVE"
     gene            3423..4853
                     /gene="leuC"
                     /locus_tag="VN034_12885"
     CDS             3423..4853
                     /gene="leuC"
                     /locus_tag="VN034_12885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWV61551.1"
                     /translation="MTRPRTLYEKIWDAHVVEQRSDGTCLIFIDRHLVHEVTSPQAFA
                     GLRASGRTVRRPDLTIAVPDHNVPTTPRLDAHGNKLPIADPESAAQLAALEKNAPEFG
                     IRYIDATAPEQGIVHVVGPEQGFSLPGTTIVCGDSHTACHGGIGALAFGIGTSEVEHV
                     LATQTLLLQPSKTMEVRVEGEVGPGVSAKDIILHITGVIGAAGGTGHVIEYTGSAVRA
                     LSVEGRLTVSNMAIEGGARAGLIAPDETTFAYLKGRPYAPKGADWDAAVAYWKSLTTD
                     PGATYDKSVVIDAADIAPSVTWGTSPEDVVPITGVVPAPDSFADPSKQTAAAKSLAYM
                     GLEPGTRMQDVPIENIFIGSCTNSRIEDLRAAAAVIKGRHKADNVKWAIVVPGSGLVK
                     AQAEAEGLDRIFTDAGLEWREPGCSACLAMNPDKVPAGERCASTSNRNFVGRQGPGAR
                     THLVSPAMAAAAAVTGKLTDVRELMA"
     gene            4850..5440
                     /gene="leuD"
                     /locus_tag="VN034_12890"
     CDS             4850..5440
                     /gene="leuD"
                     /locus_tag="VN034_12890"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWV61552.1"
                     /translation="MTPLDKVEGRAIPFGLKNVDTDVIIPAHWLKTTTREGMGRGAFE
                     SLRADPDNLFDSADFKGAPILIAGDNFGCGSSREHAAWALGDLGIRVVIAPSYSDIFS
                     GNAVKNGILPVVLPQAAIDRLMEVAETDPVFVDLEHQTVTTQFQDRFTFEIDPFRKMC
                     LLEGLDEISLTEKSDAAIGAYEAKLDADRPWMRPAA"
     gene            5472..6467
                     /locus_tag="VN034_12895"
     CDS             5472..6467
                     /locus_tag="VN034_12895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HWV61553.1"
                     /translation="MKALLSTATGGPETLTLGELPRPTAGKGQIVIDVKACAVNFPDV
                     LIIEDKYQFRPERPFAPGGEVAGLVAEIGEGVTEFKVGDRVIAGCGNGGMSEAVAVGV
                     HNVYHLPEGHDFAEGASLLMTYGTSIHALVDRGHIKEGDTLLVLGASGGVGIAAVELG
                     KAFGARVVAGVSSEEKAGIARQAGADEVVVYGRQPFDKDQSKELANKFKEAVGPNGAN
                     LVYDAVGGDYAEPALRSIAWEGRYLVVGFPAGIPRLPLNLTLLKSCDVAGVFWGAFVA
                     REPARNRANIARLFQLWEQGKISPRVTESFALEDGGKAIAKLGDRTAVGKLVVTV"
     gene            6625..6918
                     /locus_tag="VN034_12900"
     CDS             6625..6918
                     /locus_tag="VN034_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61554.1"
                     /translation="MAKRRSTSVEGDASIANISRTRRAQLVAEFEGELGVDYKARAAA
                     EEAERRWIARKTFIVWTIALMFFAIACQKLWIMGQDVDVPFSDINPVRSLFGD"
     gene            complement(6943..8757)
                     /gene="sdhA"
                     /locus_tag="VN034_12905"
     CDS             complement(6943..8757)
                     /gene="sdhA"
                     /locus_tag="VN034_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWV61555.1"
                     /translation="MTDAYKIIDHIYDTVVVGAGGSGLRATMGSAEAGLKTACITKVF
                     PTRSHTVAAQGGIAASLGNNSPDHWQWHMYDTVKGSDWLGDQDAIEYMVREAPNAVYE
                     LEHAGVPFSRNQDGTIYQRPFGGHMQNMGEGPPVQRTCAAADRTGHAMLHALYQQSLK
                     YDADFFVEYFALDLIMEDGPDGKVCRGVIALCMEDGSIHRFRSQAVVLATGGYGRTYF
                     TATSAHTCTGDGGGMVLRAGLPLQDMEFVQFHPTGIYGAGVLITEGARGEGGYLTNSE
                     GERFMERYAPSAKDLASRDVVSRSMALEIREGRGVGPHKDHIFLHLDHIDPAVLAERL
                     PGITESGKIFAGVDLTRQPLPVVPTVHYNMGGIPCNYHGEVVTLGKDGPDTVVPGLFA
                     VGEAACVSVHGANRLGSNSLIDLVVFGRATGHRLKEILTPGAAQQPLPKDSADLALAR
                     LDHFRNANGGSPTAEIRTEMQRAMSQHAAVFRTDELMAEGKEKLTKTYNRMNDVHVTD
                     RSLIWNTDLVETLELDNLISQATVTMHSAFNRKESRGAHAHEDFPNRDDANWMKHTIA
                     WFDGWGGKGGGVRLDYRPVHEYTLSDDVEYIKPKARVY"
     gene            complement(8759..9166)
                     /gene="sdhD"
                     /locus_tag="VN034_12910"
     CDS             complement(8759..9166)
                     /gene="sdhD"
                     /locus_tag="VN034_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWV61556.1"
                     /translation="MGNGTRLGRVRGLGSSHHGSHHWLQQRFTALGNVLLVGFLFVSF
                     LRLPMTDHGAVLRWASNPMVALALILMVANVFWHLRLGLQVMIEDYVHGEATKLLSLV
                     VLNFYAIGGAAYGIFAIIRIALAPAAGLAPGGM"
     gene            complement(9159..9608)
                     /gene="sdhC"
                     /locus_tag="VN034_12915"
     CDS             complement(9159..9608)
                     /gene="sdhC"
                     /locus_tag="VN034_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017180897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWV61557.1"
                     /translation="MAGNEPSARPRSPHLQVYKWTPAMAVSIFHRVSGDGLAIVGGLM
                     FLWWLGALAAGPDAYAAFLACVWHDPSPEVGTFHQITNILGKIVLIGLTWAFFQHLCS
                     GLRHFVLDTGAGYELKTNKLWSILVFVGALTATAATWLYIFAEALNG"
     gene            complement(9926..10393)
                     /locus_tag="VN034_12920"
     CDS             complement(9926..10393)
                     /locus_tag="VN034_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWV61558.1"
                     /translation="MPKLDLDTIPQTNTTGYPAPYDAPVQGRWYRRLAPATGLSDFAA
                     SHVVLKPGAWSSQRHWHDGEDEMLVMISGEAVLVEDDGRWPMRPGDIAVWPKGSTNGH
                     HLINESDADCVFVALGGGKAHDTGGGYSDIDMLFTPDGYFHKDGTPYPTSRIP"
     gene            10490..11626
                     /gene="dapE"
                     /locus_tag="VN034_12925"
     CDS             10490..11626
                     /gene="dapE"
                     /locus_tag="VN034_12925"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWV61559.1"
                     /translation="MTHAIDPVELAKTLIAAPSVTPAMGAVFDVLEAALTPLGFTVER
                     FIDGIEPDGPVENLLAVRAGTGPKHFGFAGHLDVVPPGVGWTGDAFAPEVRGNLLYGR
                     GAVDMKGSIAAFVAAAAATPAEAGTISLIITGDEEGPAIFGTRALMEHMDARGMKPDM
                     IVVGEPTSVNRLGDMVKIGRRGSVNIWIDVPGTQGHVAYPHLADNPIPKLVRILAAID
                     AVALDEGNDWFQPSNIEFTDIEVGNGATNVIPASARARLSIRFNDAHMGADLVAMIER
                     IAHEVEPNAKILGKISGEAFLTPPGELSELVAEAIHAETDLRPEMSTTGGTSDARFLH
                     ALCPVVEFGLTNATMHKLDEAVAVDDLHTLTAIYRGILMRALLD"
     gene            complement(11797..>11900)
                     /locus_tag="VN034_12930"
     CDS             complement(11797..>11900)
                     /locus_tag="VN034_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWV61560.1"
                     /translation="YSPHADQIVALRPAHISHGGAGAVYVILRRGRE"
BASE COUNT         2076 a         3867 c         3967 g         1990 t
ORIGIN      
        1 gcctggcgca cctcgctgcg cttgccgggg ggcgccgaag cgcgcctcac actcgacgac
       61 ctgctggccc agcgcaccgg cctgacccgg catgcctatg acgagaagct ggaggaaggg
      121 cgcgatcccg cgctgctgcg cgcgagcctt ggcgccgcgc cgctgcaatg cgagcccggc
      181 acctgctata cctatcagaa tgtcgccttc gatgccgcga gcgagatatt gggcgaggcc
      241 gcgaacgaga ttttcgccga cgtggtcgag aatcggtttt tccgcccgct cggcatgatc
      301 agcgcgggtt acggaatggc gcggctgacc ggcgccaagg attgggcgaa accgcaccgc
      361 ggcgcacatg ttcatccggt caaggaagcc tattggcgcg tccccgccgc cgcgggcgtc
      421 gaaagcgata tcgtcgattt cgcgacgtgg atgcaggcga tgatgggcag ccgtcccgat
      481 gtcctgccgg cgcccgcgtt gcagctggcc catcgccccc gcgtcggcac cgggcggctc
      541 tatggcggac cgctgcgtca ggccaccagc gacgcgtcct atggcctcgg ctggcggagc
      601 ttcacctatg gcgggcgccg gctcgaaggc cattcgggcg cggtcgaggg ctatcgcgcg
      661 acgatgattt tcgacccggc gacacgaacg ggcgtcgtcg cgatgtggaa cagcgattgg
      721 ggatttccgt tccgcatccc cttcgcggcg atcgacagct atcatcagcg cgaagacacc
      781 aactggctcg accttggcga attgcctccg gtgacgcgcg cccgcaccgc cgccaaagcg
      841 atcgtcgagc ccccgaaggg ataaggctcc ttgctccggc gtccatcgcc tgtgtatcag
      901 gcgcgcgacc acgatcggga ggagaatcat cgatgcgcgt gctgctgacc ggatcgtcgg
      961 gttggctcgg acgctatctg gctcctttgc tcgctaaaaa cggtcataag gtggtcgggc
     1021 tcgatgtcgt gtccggcaca tataccgagg tgatcggcac cgtcgccgac cgcgcgctcg
     1081 tcgagcgcac gatgggcgaa tatggtatcg aggcggtgat ccacggcggc gcgctgcaca
     1141 agcccgacat catccgttat ccgcggcagg ccttcatcga cgtcaatgtg acgggtacgc
     1201 tgaacttgat cgaggcggcg attgcggcgg gcaacgaccg cttcctcttc acctcgacca
     1261 cctcgctgat ggtgcgcgcc gacgtgcgtg cgggcgcggg cgaagcaggc gcctggtgga
     1321 tggacgagga tttcggtccg atcgagccgc gcaacattta tggcatcacc aagctcgcgg
     1381 cggagcaggc ctgcaagctc gttcaccgcg agcatgggat caacgtcgcg atcctgcgca
     1441 ccgggcgttt cttccccgaa gatgacgaca cgcatgcgat cccgagcggc ccgaacctca
     1501 aggcgaacga actgctcaat cgccgcctga ccgtcgaaga cgccgccgcc gcgcatctcg
     1561 ccgcgctcga ggccgcaccg gcgatcggct gcgacacctt catcctctcg gcgccgccgc
     1621 cctttgcacg cgaggaggcc gaagagctgg cccgcgacgc acgcgcggtg atcgcgcgtc
     1681 accatcccga cgccgccgag ctctacgccg ccgaaggctg gattctcccc gagagcatcg
     1741 accgcgtcta cgacccgtcg cgcgccgagc gccgtttcgg ctggcgagcc aaggcggact
     1801 tcggcagcct actcgcggcg ctccgcgacc gaacgccgct gcccttcgcc cacgaccccg
     1861 attacacctc ccccattatc gcacaggagc gcgacgcatg tatgtgctga tcaccgccaa
     1921 ccgcaattat tcgagctggt cgctccgccc gtggatgctg atgcagggcc tcgccattcc
     1981 cttcgaggat cagatcgagc ccttcaccaa tccggtcaac tatgacgaat tccgcagctt
     2041 ttcgccgacc gggcaggtgc ccgcgctgct cgacgaaggc cggacggttt atgattcgct
     2101 cggcatcacc ctctatctcg ccgaccggca cgacggcgtg tggccggccg atcccgatgc
     2161 gcgcgcctgg gcccagtgcg ccgtcgccga gatgcacagc ggcttttcgg cgctgcgcaa
     2221 tgattgcacg atgaatgtcg gtgtccgcgt gacgccgaag ccgccgtcgg gcgcgctcag
     2281 gctcgatatc gcgcgtatcc gcgagctgtt cgccgaaggg ttgtcgcgct tcggcggccc
     2341 gtatctcgcg ggcgatcgct tcaccgctgc cgatgccttc tatgcgcccg tcgccttccg
     2401 cattcgcacc tatggcctcg acgtcggcgc cgggcaggcg tgggtcgacc atatgctgac
     2461 gcatccggcg atgcaggact gggaacggca ggcgctggcc gagagctggc gcgaggaaag
     2521 ccacgaagcg gagttgatgg cagcgggtgc gatcacggcc gattaccgga cggcctgagc
     2581 ttccgtatcc ccgagcgaag acgaggggca ataccgagcg aagtcgaggc agcgacgttg
     2641 ctgttctcgc ttcgctcgaa ccagtccctc gtcttcgctc gggaatgcgg gggttttatt
     2701 cgaccagcgc ctcgcgcacc accgcgatcc ccgcctcgcg ctgcgccttg agttcggctt
     2761 tcagcttcgc cggatcgcgc gcgaagacga agccgaagct aaccccgccc tccttgccga
     2821 gcgtcgcatg gtggagcttg tgcgcctgca cgagccgctt cgcatagccg cggcggggca
     2881 cccagcggaa atagcgctgg tggacgagcc cgtcgtgcac cagcgtatag atgatgccgt
     2941 agaggaggat gccgagtccg atccacgtcc ccggttccca cgccgaggcg ccgaggatca
     3001 tcgggctgcc ggccatgaac atcgaaatgc tcatcgcggc gccgacaagg gcgaagaggt
     3061 cgttcttttc aagcacatgc ccgtgcggtt cgtgatggtc gcggtgccac gcccagccga
     3121 agccgtgcat gatatatttg tggctcgccc aagcgacgaa ttccatcgcc acgacggtga
     3181 caaggatgag cgcgaggatg gcgggcgtcg acatgcaggc gattataggc ttgtcgaaga
     3241 catgatacca caccccggtc atcccggcga aagccgggat ctcgccattg cgtttattcg
     3301 atagggtgag atcccggcct tcgccgggat gacgataatg gcaaagaagg caccacgacc
     3361 gaaaattgcg ttttcgatcg ccactcgctt gcattgttat gcgactggcg taagtggacc
     3421 gcatgactcg gccccgcacc ctttatgaga aaatctggga cgcgcatgtc gtcgaacagc
     3481 gctccgatgg tacctgcctg atcttcatcg accgccatct cgtccatgaa gtcaccagcc
     3541 cgcaggcgtt tgccgggctt cgcgcgagcg gccgcacggt gcgccgcccc gacctgacga
     3601 ttgcggtgcc cgaccataat gtgccgacga cgccgcggct cgacgcgcac ggaaacaagc
     3661 tgccgatcgc tgaccccgaa agcgcggcgc aattggcggc gctcgaaaag aacgcgcccg
     3721 aattcggtat ccgctacatc gacgcgacgg cgcccgaaca gggcatcgtc catgtcgtcg
     3781 gccccgagca gggcttttcg ctgcccggca cgacgatcgt ctgcggcgac agccacaccg
     3841 cgtgccacgg cggcatcggc gcgctcgcct tcggcatcgg gacgagcgag gtcgagcatg
     3901 tgctggcgac gcagacgctg ctgctccagc cgtcgaagac aatggaagtg cgcgtcgagg
     3961 gcgaagtcgg ccccggtgtc agcgcgaagg acatcattct ccacatcacc ggcgtcatcg
     4021 gcgccgcggg cggcaccggc catgtcatcg aatataccgg cagcgcggtc cgcgcgctgt
     4081 cggtcgaagg ccggctgacc gtcagcaaca tggcgatcga gggtggcgcg cgcgccggcc
     4141 tgatcgcgcc cgacgagacg acattcgcgt acctcaaggg ccgcccctat gcgccgaagg
     4201 gtgccgactg ggacgccgcg gtcgcttatt ggaaaagcct cacgaccgat ccgggtgcga
     4261 cctatgacaa gagtgtcgtc atcgacgccg ccgacatcgc accgagcgtc acctggggca
     4321 ccagccccga ggacgtcgtg ccgatcaccg gcgtcgtgcc cgcgcccgac agcttcgccg
     4381 acccgtcgaa gcagaccgcg gccgccaaat cgctcgctta tatgggactc gaacccggca
     4441 cgcggatgca ggatgtgccg atcgagaata tcttcatcgg cagttgcacc aacagccgga
     4501 tcgaggatct gcgcgccgcc gcggcggtga tcaagggccg gcacaaggcc gacaatgtca
     4561 aatgggcgat cgtcgtcccc ggatcggggc tggtgaaagc gcaggccgag gccgaggggc
     4621 tcgaccgcat cttcaccgac gccggcctcg aatggcgcga gcccggctgt tcggcgtgtc
     4681 tcgcgatgaa ccccgacaag gtgcccgcgg gcgaacgctg cgcctcgacg agcaaccgca
     4741 atttcgtcgg ccgccagggc cccggcgcgc gcacgcatct ggtcagcccc gcgatggcgg
     4801 cggcggcggc ggtgacgggc aagctcaccg acgtcaggga gttgatggca tgaccccgct
     4861 cgacaaggtc gaaggccgcg cgattccgtt cgggctcaaa aatgtcgaca ccgacgtcat
     4921 cattcccgcg cactggctga agacgacgac gcgcgagggc atggggcgcg gcgcgttcga
     4981 aagcctgcgc gccgatcccg acaatctgtt cgacagcgcc gatttcaagg gcgcgccgat
     5041 cctgatcgcg ggcgacaatt tcggctgcgg atcgagccgc gaacatgccg cctgggcgct
     5101 cggcgacctt ggcatccgtg tcgtgatcgc gccctcctat tcggacatct tctcgggcaa
     5161 tgcggtgaag aacggcatcc tccccgtcgt gttgccgcaa gccgcgatcg accggttgat
     5221 ggaggttgcc gaaaccgatc cggtgttcgt cgacctcgag catcagacgg tgacgacgca
     5281 gtttcaggac cgctttactt tcgagatcga tccgttccgc aaaatgtgcc tgctcgaagg
     5341 gctcgacgag atttcgctga ccgaaaagag tgacgcggcg atcggcgcct acgaggccaa
     5401 actcgacgcc gaccggccgt ggatgcggcc cgccgcatag gatcaaacgc acaccataag
     5461 aggagaagat gatgaaggct ctcttgtcga ccgccacggg cggccccgaa acgctgacgc
     5521 tcggcgaatt gccgcgcccg accgcgggca agggccaaat cgtgatcgac gtgaaggcct
     5581 gcgcggtcaa ctttcccgac gtgctcatca tcgaggacaa gtaccagttc cgccccgaac
     5641 gccccttcgc ccccggcggc gaagtcgcgg gcctagtggc cgagatcggc gagggcgtca
     5701 ccgaattcaa ggtcggcgac cgggtgatcg cgggctgcgg caacggcggc atgtccgaag
     5761 cggtcgcggt cggcgtccac aatgtctatc acctgcccga aggccatgac ttcgccgagg
     5821 gtgcgtcgct gctgatgacc tatggcacca gcatccacgc gctcgtcgat cgcggccata
     5881 tcaaggaagg cgacaccttg ctcgtgctcg gcgcgtcggg cggggtcggc atcgccgcgg
     5941 tcgaactggg caaggcgttc ggtgcgcgcg tcgtcgcggg cgtgtcgagc gaagaaaagg
     6001 ccgggatcgc gcgtcaggcc ggcgccgacg aggtcgtcgt ctatggccgc cagccgttcg
     6061 acaaggacca gtcgaaggag ctcgcgaaca agttcaagga agccgtcggt ccgaatggcg
     6121 cgaacctcgt ctacgacgcg gtcggcggcg actatgccga gcccgcgctg cgctcgatcg
     6181 catgggaggg ccgctatctc gtcgtcggct tccccgcggg catcccgcgc ctgccgctca
     6241 acctgacctt gctcaagagc tgcgatgtcg cgggcgtttt ctggggcgcg ttcgttgcgc
     6301 gcgagcccgc gcgcaaccgc gccaatatcg cgcggctgtt ccagctgtgg gagcagggca
     6361 agatcagccc gcgcgtcacc gaaagcttcg cgctcgaaga cggcggcaag gcgatcgcca
     6421 agctcggcga ccgcaccgcg gtcggaaagc tggtcgttac cgtctgaaac actggcccga
     6481 cccggcgctg accgcgggcg cagcgcgcgc caccgcgcgc ctcacgccga tcgcaattgc
     6541 gacgagccga tagcgccggg ttaaccaatt ggcaggggct cggcgttata ccgggggcca
     6601 ggactagctg ggccaaggtg gatcatggca aaacgccgat cgacaagcgt tgagggtgat
     6661 gcgagcatcg cgaatatttc gcgcacccgc cgcgcgcagc ttgtcgccga attcgaaggc
     6721 gagctcggcg tcgactacaa ggcccgcgcg gcggccgaag aggccgaacg ccgctggatc
     6781 gcgcgcaaga ccttcatcgt ctggacgatc gcgctgatgt tctttgccat cgcgtgccag
     6841 aaattatgga tcatgggtca ggacgtcgac gtgccctttt ccgacatcaa cccggtgcgc
     6901 agcctgtttg gggattagag ccggccggca ccccggcgcg gatcagtaaa cccgcgcctt
     6961 cggcttgata tattcgacgt cgtcggacag cgtatattcg tggaccgggc ggtaatcgag
     7021 gcggacgccg ccgcccttgc cgccccagcc gtcgaaccag gcgatcgtgt gcttcatcca
     7081 gttcgcgtcg tcgcggttcg ggaaatcctc gtgcgcgtgg gcgccgcggc tttccttgcg
     7141 gttgaacgcc gagtgcatcg tcaccgtcgc ctggctgatc agattgtcga gctcgagcgt
     7201 ttcgacgagg tcggtgttcc agatcaggct gcggtcggtg acatgaacgt cgttcatgcg
     7261 attgtaggtc ttggtgagct tttccttgcc ttcggccatc agctcgtcgg tgcggaacac
     7321 cgccgcatgc tgcgacatcg cgcgctgcat ttcggtgcgg atctcggcgg tcggcgagcc
     7381 gccgttggca ttgcggaaat ggtcgagccg cgcgagcgcg aggtcggcgc tgtccttggg
     7441 cagcggctgc tgcgcggcgc cgggggtcag gatttccttg aggcggtgcc cggtcgcgcg
     7501 gccgaagacg acgaggtcga tcaggctgtt cgagccgagg cggttcgcgc catggaccga
     7561 cacgcacgcc gcttcgccga ccgcgaacag gccgggaacg actgtatcgg ggccgtcctt
     7621 gccgagcgtg acgacttcgc catgatagtt acaggggatg ccgcccatat tgtaatggac
     7681 cgtcggcacg acggggagcg gctggcgcgt caggtcgacg ccggcgaaaa tcttgccgct
     7741 ctcggtgatg ccgggcaggc gctcggcgag caccgcggga tcgatatgat cgaggtgcag
     7801 gaagatatgg tccttgtgcg ggccgacgcc gcgaccttcg cggatttcta gcgccatcga
     7861 gcgcgagacg acgtcgcgcg aggcgaggtc cttcgccgac ggggcatagc gttccatgaa
     7921 ccgctcgcct tcgctgttgg tgagatagcc gccctcgccg cgcgcgcctt cggtgatgag
     7981 gacgcccgcg ccgtaaatgc cggtcgggtg gaactggacg aactccatat cctgcagcgg
     8041 caggccggcg cgcagcacca tgccgccgcc atcgccggtg caggtgtgcg ccgaggtcgc
     8101 ggtgaaatag gtgcggccgt agccgcccgt cgcgagcacg accgcctggc tacggaaacg
     8161 gtggatgctg ccatcctcca tgcagagggc aatcacgccg cggcagacct tgccgtccgg
     8221 accgtcttcc atgatcaggt cgagcgcgaa atattcgacg aagaagtccg cgtcatattt
     8281 cagcgactgc tgatagagcg cgtggagcat cgcgtggccg gtgcggtcgg cggcggcgca
     8341 ggtgcgctgc accggcgggc cctcgcccat attctgcata tggccgccga agggccgctg
     8401 gtagatggtg ccgtcctgat tgcgcgaaaa cggaacgccg gcgtgctcga gctcgtagac
     8461 cgcgttcggc gcctcgcgca ccatatattc gatcgcgtcc tgatcgccga gccagtccga
     8521 ccccttgacg gtgtcgtaca tgtgccattg ccaatggtcg ggcgaattgt tgccgagcga
     8581 cgcggcgatg ccgccctgcg ccgcgacggt gtggctgcgc gtcggaaaca ccttggtgat
     8641 gcaggcggtc ttgagccccg cttcggcgct gcccatcgtg gcgcgcaggc ccgagccgcc
     8701 ggcaccgacg acgacggtgt cgtagatatg gtcgatgatc ttgtaggcgt cggtcatctt
     8761 acatgccccc aggagcgagc ccggcggcgg gcgcaagcgc gatgcggatg atggcgaaga
     8821 tgccgtaagc ggcgccgccg atcgcgtaga agttcaaaac gacgagcgac agcaatttgg
     8881 tcgcctcgcc gtggacatag tcctcgatca ttacctgcag cccgaggcga aggtgccaga
     8941 agacgttggc gaccatcagg atcagcgcga gcgcgaccat cggattcgac gcccagcgca
     9001 gcaccgcgcc atggtcggtc atcggcagac gcaggaagga aacgaagaga aagccgacga
     9061 gcaggacatt gccgagcgcg gtgaagcgct gctgcagcca gtggtgcgaa ccatggtgcg
     9121 acgagccgag cccgcgcacg cggccgagac gcgttccgtt acccattgag cgcctccgca
     9181 aagatataga gccaggtggc ggcggtcgcg gtgagcgcgc cgacgaaaac gaggatcgac
     9241 cagagcttgt tggtcttgag ctcatagccc gcgccggtgt cgagcacgaa atggcgcagc
     9301 cccgagcaaa gatgctggaa gaaagcccag gtcaggccga tcagcacgat tttgccgaga
     9361 atgttggtga tctggtgaaa ggtgccgacc tcggggctcg gatcgtgcca cacgcaggcg
     9421 aggaaggccg cataggcgtc ggggcccgcg gcgagcgcgc cgagccacca gaggaacatc
     9481 agcccgccga cgatcgcgag accgtcgcca ctgacgcggt gaaagatgga aaccgccatc
     9541 gcaggggtcc atttatacac ctgcaaatgc ggggagcgcg gacgcgcgct tggttcgttg
     9601 ccagccatat cagccctgtc ttgactctaa acggtcgatg cgccgccggt taagccttcc
     9661 cgccgcgata tgcaattgct taggagcata ggggaataaa agctttggga cgggcgatat
     9721 gggctcgacc ggtcgcggac atattctccc ctcccgcttg cgggaggggt tgggggtggg
     9781 cagcggcgtc gcgatggccc accccgctgc gactaacgaa caaagttcgt aagtctctct
     9841 gcccctcccg cctgcgggag gggaaagcag atcaacccct cagcggcagc tctccacctc
     9901 aaagctccca ccgcagttcc gccgatcacg gtattcgcga cgtcggatag ggcgtgccgt
     9961 ccttgtggaa atagccgtcg ggcgtgaaga gcatgtcgat gtccgaatag ccgccgccgg
    10021 tgtcgtgcgc cttgccgccg ccgagcgcga cgaagacgca gtcggcatcc gattcgttga
    10081 tcaggtgatg accgttggtg ctgcccttcg gccacaccgc gatatcgccc gggcgcatcg
    10141 gccagcggcc gtcatcctcg accagcaccg cctcgcccga aatcatcacg agcatctcgt
    10201 cctcgccgtc gtgccagtgg cgctgcgacg accaggcgcc gggtttgagc accacgtggc
    10261 tcgcggcgaa gtcgctgagc cccgtggcgg gcgcgaggcg gcgataccaa cggccctgca
    10321 cgggcgcatc gtaaggcgcg ggatagccgg tggtgttggt ttgcgggatc gtatcgagat
    10381 cgagcttggg catggcagtc ctcgtgagtt tgttcctcct ttgtttcgca caaagggcgc
    10441 gcgatgcaaa gcctcgactt ggcgcgccgc gaaggagtaa gggggcggca tgacccatgc
    10501 tatcgacccc gtcgaactcg ccaagacgct gatcgccgcg ccgagcgtca cccccgccat
    10561 gggcgccgtg ttcgacgtgc tcgaggccgc gctgaccccg ctgggcttta ccgtcgagcg
    10621 cttcatcgac gggatcgagc ccgacgggcc ggtcgagaat ctgctcgcgg tgcgcgcggg
    10681 gacgggaccg aaacatttcg gctttgccgg gcatctcgac gtcgtgccgc cgggcgtcgg
    10741 ctggaccggg gacgcctttg cgcccgaagt gcgcggcaat ctgctctatg gccgcggcgc
    10801 ggtcgacatg aagggttcga tcgcggcgtt cgtcgctgca gcggccgcga cgccggccga
    10861 agccggcacg atcagcctga tcatcaccgg cgacgaggag ggtccggcga tcttcggcac
    10921 gcgcgcgctg atggaacata tggacgcgcg cggcatgaag cccgacatga tcgtcgtcgg
    10981 cgagccgacg tcggtgaacc ggctcggcga catggtgaag atcgggcggc gcggctcggt
    11041 caatatctgg atcgacgtcc cggggacgca gggacatgtc gcctatccgc acctcgccga
    11101 caatccgatc cccaagctgg tgcggatatt ggcggcgatc gacgcggtgg cgctcgatga
    11161 gggcaatgac tggttccagc cgtcgaacat cgaatttacc gacatcgagg tcggcaatgg
    11221 cgcgaccaac gtcatccccg cgtcggcgcg cgcgcggctg tcgatccgct tcaatgacgc
    11281 ccacatgggt gccgacctcg tcgcgatgat cgagcggatc gcgcatgagg tcgaacctaa
    11341 cgccaagata ttgggcaaga tttcgggcga ggcctttctg acaccgccgg gcgagctgtc
    11401 cgagctggtc gccgaggcga tccatgccga gaccgatttg cgtcccgaaa tgtcgacgac
    11461 gggcggcacg tcggacgcgc gcttccttca cgcgctctgc cccgtagtcg agttcgggct
    11521 gaccaatgcg acgatgcaca agctagacga ggcggtcgcg gtggacgatc tgcacacgct
    11581 gaccgccatc tatcgcggga ttttgatgcg ggcgctgctg gattagtggg ttcgcgcaaa
    11641 gacgcaaaga cacgaagaag ccggcgcggg ccgtcaggtc cgcttttcca ttgacgatgc
    11701 gccgggtggc aatgccagag agggaaagcc gcttcgcggg aaacacccct tcttcgtgtc
    11761 tttgcgtctt tgcgtctttg cgcgaaattc ttctttttac tcccgccccc gccgcagaat
    11821 gacatagacc gcccccgccc cgccgtggct gatatgcgct gggcggagcg cgacgatctg
    11881 gtcggcgtgg ggcgaatagg
//
LOCUS       DATMSU010000487         5344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_178842,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000487 DATMSU010000000
VERSION     DATMSU010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178842"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VN034_12935"
     CDS             <1..863
                     /locus_tag="VN034_12935"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWV61561.1"
                     /translation="AGSYADYVLPGLTFYERADLPMVPFMSMVQPFLQYAEPVIAPVG
                     EARSEFDTFCDILARMGLRMPAASPDEVAAQAAGGQMRPLEVLDGAIRQGPAGMHGDW
                     SLDKLRDHPHGVMLDIPIPWGNQEKIAHADGRIHLWDDMVAAELDRLFAAAPVDGLKL
                     LSRRDMRSHNGWLHNVDRLIRSQKPTLHIHPDDAAERGLSDGDRATLSNRFGAIEVEV
                     EINDDHRRGTVSYPHCFGHGTGGWQRANRAGGANVNILLGHGPDVVEAVSGTTLMDGI
                     AVELTALHPA"
     gene            complement(866..1492)
                     /locus_tag="VN034_12940"
     CDS             complement(866..1492)
                     /locus_tag="VN034_12940"
                     /inference="COORDINATES: protein motif:HMM:NF025867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWV61562.1"
                     /translation="MTIKAARREERSASAEALLNATAQLLSEATTIDVSLNEISQRAS
                     VNSAMIKYYFGNKEGLLLALLERDAETAMTALKALSEMEIPAQKKLKIHINGIINAYY
                     RSPYINRLIHYMVESGSPAASKRVAQIFIEPMIDVYRDIVAQGVSEGVLKEVDPGLLY
                     YCLVGAADHIFHAGYSVPSTLGVDRLDDGIKQKYAELVCDIYLRGLAP"
     gene            complement(1537..1857)
                     /locus_tag="VN034_12945"
     CDS             complement(1537..1857)
                     /locus_tag="VN034_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020043927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWV61563.1"
                     /translation="MASVNFHNADGSVETVEIAVGNSVMRGARDNMVEGIEADCGGVC
                     ACATCHIYIAPEWVERVGPAGVDESEMLDCVNDPRPNSRLSCQIAMTDELEGLTVFLP
                     ESQR"
     gene            1986..3590
                     /locus_tag="VN034_12950"
     CDS             1986..3590
                     /locus_tag="VN034_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWV61564.1"
                     /translation="MLGEEADYIVVGGGSAGCVLAARLSENPAVRVALVEAGGESTGL
                     MVQMPVGFAKMLVDDRYDWKYWQLPDESINGRCFIWSGGRMLGGGSSINGQVYIRGTR
                     ADFDAWERLGATGWNFDGVFPYFLRSETWSGAPNQNHGSQGPLSVSPMRDPHPLCDLF
                     LRACEQYGLARLDDYNGGQMEGAYLTQATQRDGWRCSTEKAYLRDARGRPNLMTITNA
                     HVETILIENGEAVGIRYRQGGDAKVLRARREVIVASGAMGSPALLLRSGIGAGDYLQA
                     RGIAVQADRREVGHNLQEHPGITQNKFVSVPTLNSQVGPLDMVRHLAKFFWNKSGPMG
                     APAVQAMGLARTREGLEEPDVQLHFMPLAYNVEPDTVSSAEAVMPKEPCVSINVSLTR
                     PKSRGRVELGDALEPVINHKLLGERADVDTLIGAMKLVDKLFAMPALSAITLGDRSPD
                     PVPGDDAGWEEYVRAKAMITYHPVGSCRMGSDADSVVDPECRVRGVGRLRVADASIMP
                     QITSGNTNAATIMIGEKAAEMIRTAA"
     gene            3587..4360
                     /locus_tag="VN034_12955"
     CDS             3587..4360
                     /locus_tag="VN034_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV61565.1"
                     /translation="MSEAAAYDFRHIAVERPMPGVVLLTLNRPERMNATNDALHSELV
                     RLPALIDADPEARAAVVTGAGRAFCVGGDWADIAGAGNDYANKIRMMRETSQILTALI
                     EMRKPLVSAINGPAAGIGLALGLLADISVVNEAANLSDGHLRIGLAAGDHAAMVWPLL
                     CSMAKAKRYLLTGDKLSGREAERIGLVSDAVPPAEVLPLALHYAASLAERSPLAVELS
                     KRALNHWLRDAMPIFEASLGYEMVTAFDPVQAAHMPSSS"
     gene            4371..5147
                     /locus_tag="VN034_12960"
     CDS             4371..5147
                     /locus_tag="VN034_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWV61566.1"
                     /translation="MTTEPTLIVDTPAPGVLHLIFNRPEVHNAYDMATFSAFAEQLDR
                     AATDPEIRAIVIGARGGRAFSAGFDIHEMAGFDADTMRRAFESRDPVFGRVTAHPLPV
                     IAAIDGICFGAGALLALACDMRIATPAFRFKVTAVGYGGANATWSLPRAVGAARAKAI
                     LMTGQVVEAAEAERIGLVNEIVEASELDARALALAATIAANPPQGVAGIKTLVDGSLT
                     RSPIEGWQAEYDWMIATMTDAPGGGETFGKFLSEHDDHKR"
BASE COUNT          919 a         1711 c         1783 g          931 t
ORIGIN      
        1 aggccggctc ctatgccgat tatgtgctgc cgggcctgac cttctacgag cgcgccgatc
       61 tgccgatggt gccgttcatg agcatggtgc agccgttcct gcaatatgcc gagcccgtga
      121 tcgcgccggt cggcgaggcg cggagcgagt tcgacacctt ctgcgacata ttggcgcgca
      181 tggggctgcg catgcccgcc gcgtcgcccg acgaggtcgc ggcgcaagcg gcgggcggtc
      241 agatgcgccc gctcgaggtt ctcgacggcg cgatccggca ggggccggcg ggcatgcatg
      301 gcgactggtc gctcgacaag ctccgcgacc atccgcatgg cgtgatgctc gacatcccga
      361 tcccctgggg caatcaggag aagatcgcgc acgccgacgg caggattcac ctgtgggatg
      421 atatggttgc cgccgaactc gaccggctgt tcgcggcggc gccggtcgac ggtcttaaac
      481 tgctttcaag gcgcgacatg cgctcgcaca atggctggct ccacaatgtc gaccggctga
      541 tccgttcgca aaagccgacg ctgcatatcc atcccgacga tgccgccgaa cgcggcctct
      601 cagacggcga ccgcgcgacg ctgtcgaacc ggttcggggc gatcgaggtc gaggtcgaga
      661 tcaatgacga ccaccggcgc ggcaccgtct cctatccgca ttgcttcggt cacggcaccg
      721 gcggctggca gcgtgccaac cgcgctgggg gcgccaatgt gaacatcctg ctcggccacg
      781 gccccgacgt ggtcgaggcg gtgtcgggga cgacgctgat ggacgggatc gcggtcgaac
      841 tgacggcgct gcacccggcg tgatgtcagg gtgcgaggcc gcgcagatag atgtcgcaga
      901 ccagctcggc atatttctgc ttgatgccgt cgtcgagccg gtcgacgccg agcgtcgagg
      961 gcaccgaata gcccgcgtgg aaaatatggt ccgccgcacc gacgagacaa taatagagca
     1021 ggccggggtc gacctccttg agcacgcctt cgcttacccc ctgcgcgacg atgtcgcgat
     1081 agacgtcgat catcggctcg atgaagatct gcgcgacgcg cttgcttgcc gcggggcttc
     1141 cggactcgac catatagtgg atcagccggt tgatataggg cgagcgataa taggcgttga
     1201 tgatgccgtt gatgtggatc ttcagcttct tttgcgcggg aatttccatt tcgctcaagg
     1261 ccttgagcgc ggtcatcgcg gtttcggcat cgcgttcgag cagcgcgagc agcaaccctt
     1321 ccttgttgcc gaaataatat ttgatcatcg ccgaattgac cgacgcgcgc tgcgaaatct
     1381 cgttcagcga aacgtcgatc gtcgttgcct cggacagaag ctgcgccgtc gcgttcaaaa
     1441 gcgcctcggc cgatgccgaa cgttcctcgc gccgggctgc cttgatcgtc aagccgctct
     1501 ctccatccat gcctgtgtcc gatttattcg cccggcctac cgttgggact cgggaaggaa
     1561 gactgtcaag ccctccagtt cgtcggtcat cgcgatctgg caggaaaggc ggctgttcgg
     1621 gcgcgggtcg ttgacgcagt cgagcatctc gctttcgtcg acgccggcgg ggccgacgcg
     1681 ctccacccat tcgggggcga tgtagatgtg gcaggtcgcg cacgcgcaga cgccgccgca
     1741 atcggcctcg atcccctcga ccatattgtc gcgcgcgccg cgcataaccg agtttccgac
     1801 tgcaatttcg acggtctcga cgctgccgtc ggcattatgg aaattgacgc tcgccatatg
     1861 gtttttcctg tcccggtcgc tgtggatcaa cccactttca attgatcaat taatattgca
     1921 tggtgaccgt gtcaacgaac gcgcctatgg agcgtaaaat taattagtcg attgatggag
     1981 gatggatgtt gggcgaggag gcggactata tcgtcgtggg cggaggaagc gcgggctgcg
     2041 tgctggcggc gcggctgagc gaaaatcccg cggtccgcgt cgcgctggtc gaggcgggcg
     2101 gcgaatcgac cggattgatg gttcagatgc ccgtcggctt cgcgaaaatg ctcgtcgatg
     2161 accgctatga ctggaaatat tggcagctcc ccgacgaatc gatcaacggg cggtgcttca
     2221 tctggtcggg cgggcgaatg ctgggcggcg gcagctcgat caacggtcag gtctatattc
     2281 gcggcacccg cgccgatttc gacgcatggg agcggctggg cgcgacgggc tggaatttcg
     2341 acggcgtctt tccctatttc ctgcgcagcg agacgtggag cggcgcgccg aaccagaatc
     2401 acggctcgca agggccattg tcggtatcac cgatgcgcga cccgcatccc ttgtgcgacc
     2461 tgttcctgcg cgcgtgcgag caatatgggc tcgcgcggct cgacgattat aacggcgggc
     2521 agatggaggg cgcatatctc acgcaggcga cgcagcgcga cggctggcgg tgcagcaccg
     2581 aaaaggccta tctgcgcgat gcgcggggcc gtccgaacct gatgacgatc accaacgcgc
     2641 atgtcgaaac gatcctgatc gagaatggcg aggcggtcgg catccgctat cgccagggcg
     2701 gcgatgccaa ggtcctccgc gcccgccgtg aggtgatcgt cgcgtcggga gcgatgggtt
     2761 cgcccgcctt gctgctccgc tcgggcatcg gcgcgggcga ttatctgcag gcacgcggga
     2821 tagcggtgca ggccgaccgg cgcgaggtcg gccataatct gcaggagcat cccggcatta
     2881 cccagaacaa gttcgtcagc gtgccgacgc tcaacagcca ggtcgggccg ctcgacatgg
     2941 tccggcacct tgccaaattc ttctggaaca agtccggccc gatgggcgcc cccgccgtgc
     3001 aggcgatggg ccttgcgcga acgcgcgaag ggctcgaaga acccgacgtc cagctccact
     3061 tcatgccgct cgcctataat gtcgagcccg atacggtgtc gtcggccgag gcggtgatgc
     3121 ccaaggaacc atgcgtcagc atcaacgtgt cgctgacgcg gccgaaatcg cgcggccggg
     3181 tcgaactcgg cgacgcgctc gagccggtga tcaatcataa attgctcggc gagcgcgccg
     3241 acgtcgacac gctgatcggc gcgatgaagc tggtcgacaa gctgttcgcg atgcccgcat
     3301 tgagcgccat cacgctcggc gaccggtcgc ccgacccggt gccgggcgac gacgcgggct
     3361 gggaggaata tgtccgcgcc aaggcgatga tcacctacca cccggtcggc agctgccgca
     3421 tgggatcgga cgcggactcg gtcgtcgatc ccgaatgccg tgtgcgcggc gtcgggcggc
     3481 tgcgcgtcgc cgacgcctcg atcatgccgc agatcacgag cggcaacacc aatgccgcga
     3541 cgatcatgat cggcgaaaag gcggccgaga tgatccgcac cgccgcatga gcgaggccgc
     3601 cgcctatgat tttcggcata tcgcggtcga gcgcccgatg ccgggcgtgg tcctgctgac
     3661 actgaaccgc cccgaacgga tgaatgcgac caacgatgcg ctgcacagcg aactcgtccg
     3721 cttgcccgcg ctgatcgacg ccgatcccga ggcgcgcgcc gcggtcgtga cgggggcggg
     3781 gcgggccttt tgcgtcggcg gcgactgggc cgacatcgcg ggcgcgggca atgattacgc
     3841 caacaagatc cggatgatgc gcgaaacatc gcagattctg accgcgctga tcgagatgcg
     3901 caaaccgctc gtgtcggcga tcaacggccc cgctgccgga atcggcctcg cgctcggcct
     3961 gcttgccgat atttcggtgg tgaacgaagc ggcgaacctc tccgacgggc acctccgcat
     4021 cggcctcgcc gcgggcgatc atgccgcgat ggtctggcct ctgctctgct cgatggcgaa
     4081 ggcgaagcgt tatctgctca ccggcgacaa actcagcgga cgcgaggccg aacggatcgg
     4141 gcttgtgtcc gacgccgtgc cgcccgccga ggtactgccg cttgcgctgc attatgccgc
     4201 gtcgctcgcc gagcggtcgc cgctcgcggt cgagctcagc aagcgcgcgc tcaatcattg
     4261 gctgcgcgat gccatgccga ttttcgaagc gtcgctcggt tatgaaatgg tgaccgcctt
     4321 cgaccccgtt caggcggcgc atatgccgtc cagcagttga ggatctgccg atgaccaccg
     4381 aaccgacgct gatcgtcgac acccccgcgc cgggtgtgct gcacctgatt ttcaaccggc
     4441 ccgaggtgca caacgcctat gacatggcga ccttctcggc cttcgccgaa cagctcgacc
     4501 gcgctgcgac cgatcccgag atccgcgcca tcgtgatcgg cgcgcgcggg ggcagggcgt
     4561 tcagcgccgg cttcgacatc cacgaaatgg ccgggttcga cgccgatacg atgcgccgcg
     4621 ctttcgagag ccgcgacccg gtgttcgggc gcgtcaccgc gcatccgctg ccggtcatcg
     4681 ccgcgatcga cggtatctgt ttcggcgccg gggcgttgct cgcgcttgcc tgcgacatgc
     4741 gcatcgcgac gccggccttc cgcttcaagg ttaccgcggt cggctatggc ggcgccaatg
     4801 cgacctggtc gctcccccgg gcggtcggcg cggcgcgcgc gaaggcgata ttgatgacgg
     4861 ggcaggtcgt cgaagccgcg gaagccgaac gcatcgggct ggtgaacgaa atcgtcgagg
     4921 cgtccgaact cgacgcacgc gcgctcgcgc tggccgccac tatcgccgcc aatccgccgc
     4981 agggggtcgc aggcatcaag acactcgtcg acggcagcct gacgcgctcg ccgatcgagg
     5041 gttggcaggc cgaatatgac tggatgatcg ccaccatgac cgatgcgccc ggcggcggcg
     5101 agaccttcgg caaattcctg tccgagcacg acgaccacaa acgctgaata tgacatcagg
     5161 agagtagaat gatcggccaa gccaacgcgc aagtggcgca tcccgaccgg ctctttatcg
     5221 gcgggcgctg gatccccgtc gagggcagcg gccgctttac ggtgatcaat ccggcgaccg
     5281 aagaggcgtt cggcgaggtg gtcgcggcgg gcgcgcccga ggtcgagcgc gcggtggcgg
     5341 cggc
//
LOCUS       DATMSU010000488         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_74737, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000488 DATMSU010000000
VERSION     DATMSU010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74737"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VN034_12965"
     CDS             complement(<1..544)
                     /locus_tag="VN034_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV61567.1"
                     /translation="MIRLLAAALALAAATPAAAQTVAITGGRVVTMGPTGEIERGTVV
                     ITGGRIVAVGADVAVPAGAKIVDATGKIVTPGLIAAGTALGLIEVRAVETTDDRATDA
                     SDVSAAFDAGYGLNPDSLLIPVARLGGITRAIATPAYKDKGGRELLFAGQAAAVVLDG
                     RPMLMKRGVAMILDMGDAGAE"
     gene            complement(541..1917)
                     /locus_tag="VN034_12970"
     CDS             complement(541..1917)
                     /locus_tag="VN034_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWV61568.1"
                     /translation="MTKYLGGACIALISLTTAGAALAAHGALDDPFPSTYHPAASAPL
                     AIVGATVLTGTGAEIADATVLIAGGKIEAVGTALPVPEGYREVDGRGKWLTPGIIDAH
                     SHLGVYPSPATPSHDDGNEAVSPNTAEVWAEHSVWPQDPKFRLARAGGVTSLMVLPGS
                     ANLFGGRSVTLKNVPSVTTQGMKFPGAPYGLKIACGENPKRVYGGKGRSPATRMGNVA
                     GYRKAWIDAADYVRRWDKWEAGEKAGDPPKRDLQLETLAGVLRGEILVQNHCYRADEM
                     VVMVDVAREFGFKIRTFHHANEAFKIAPLLAKEDICVATWANSWGAKMESLDGIEENA
                     PMVDAAGGCAIIHSDDGLLIQRLGQEAGIAMTAANHAGLSVSRGEAIKWITANPAKAM
                     GIADKVGTLEPGKMADVVLWSADPFSVYALADRVWIDGVIQWDRADPRYQSPSDFVLG
                     QPGQGFAR"
     gene            2081..>2580
                     /locus_tag="VN034_12975"
     CDS             2081..>2580
                     /locus_tag="VN034_12975"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWV61569.1"
                     /translation="MWNDNRLARRAAHLSTQLPAPHFPSEFGDAIILFDSGFAAPQLL
                     PDLSRFAAEALTHHREEVLQYASGRGQPYLREWLANWMNEDGCALTADHILITNGAKQ
                     GIDLICRLLLDEGDAIVVTAPTYFTAIPIFRNYGAEFIEVGQDGAGLDVAALDAALDA
                     RAAAGKA"
BASE COUNT          408 a          919 c          811 g          442 t
ORIGIN      
        1 gctcggcgcc ggcatcgccc atgtcgagta tcatcgctac cccgcgcttc atcagcatcg
       61 gccggccatc gagcacgacc gccgccgcct gccccgcgaa gagcagttcg cggccaccct
      121 tgtccttata ggccggggtc gcgatcgcgc gcgtgatgcc gccgagccgc gcaaccggga
      181 tcagcagcga gtcgggattg agcccatagc cggcatcgaa cgcggcgctg acgtcgctcg
      241 cgtcggtagc gcgatcgtcg gtcgtctcga ccgcgcgcac ctcgatcagc ccgagcgcgg
      301 tgcccgccgc gatcaggccc ggggtcacga tcttgcccgt ggcgtcgacg attttcgccc
      361 cggccggcac cgcgacatcg gcaccgaccg cgacgatccg gccgcccgtg atcacgaccg
      421 taccccgctc gatctcgcca gtgggcccca tggtaaccac gcggcctccc gtgatcgcga
      481 cagtctgcgc ggctgcaggc gtcgccgcag cgagcgccag cgcggcggca agcaggcgga
      541 tcatcgcgcg aacccctgcc ccggctggcc gagcacgaaa tcggacggac tttgatagcg
      601 cggatcggcg cggtcccact gaatcacgcc gtcgatccag acacggtcgg caagcgcata
      661 gacgctgaaa gggtcggcgc tccacaacac gacatcggcc atctttcccg gttccagcgt
      721 gcccaccttg tcggcgattc ccatcgcttt cgccggattg gcggtgatcc atttgatcgc
      781 ctcgccgcgg ctgacggaca agccggcgtg gttggcggcc gtcatcgcga tgcccgcctc
      841 ctggcccagc cgctggatca acagaccgtc gtccgaatga ataatcgcgc agccgcccgc
      901 ggcgtcgacc atcggcgcat tctcttcgat cccgtcgagc gattccatct tcgcgcccca
      961 actgttcgcc caggtcgcga cgcaaatatc ctcctttgca agcaggggcg cgatcttgaa
     1021 cgcctcgttg gcgtggtgga aggtgcggat cttgaacccg aactcgcgcg cgacatcgac
     1081 catcacgacc atctcgtcgg cgcggtagca atggttctga acgagaatct cgccgcggag
     1141 gacgccggcg agcgtttcga gctgcaggtc gcgcttcggc gggtcgcccg ccttctcgcc
     1201 cgcctcccat ttgtcccagc ggcggacata gtcggcggca tcgatccatg ccttgcgata
     1261 acccgcgaca ttgcccatcc gcgtcgctgg cgaacgcccc ttgccgccat agacgcgctt
     1321 cggattctcg ccgcacgcga tcttgaggcc atagggagcg cccggaaact tcattccttg
     1381 cgtcgtcacc gacggcacgt tcttgagcgt gaccgaccgg ccgccgaaca gattggccga
     1441 accgggcagg accatcaggc tggtcacccc gcctgcccgc gcgaggcgga acttggggtc
     1501 ctgcggccac accgaatgtt cggcccagac ctcggcggtg ttcggcgaca ccgcttcatt
     1561 gccgtcgtcg tgcgacggcg tcgcgggcga tggatagacg ccgaggtgcg aatgcgcgtc
     1621 gatgatgccg ggggttagcc atttgccgcg gccgtcgact tcgcggtagc cttcgggcac
     1681 cgggagcgcc gtccccaccg cttcgatctt gccgcctgcg atcagcaccg tcgcgtcggc
     1741 gatttcggcc cctgtgccgg tgagcacggt cgcgccgacg atcgccagcg gcgccgatgc
     1801 ggcgggatga taggtgctgg gaaaaggatc gtcgagggcg ccgtgcgccg cgagcgccgc
     1861 accggcggtc gtcagcgata tcagcgcgat gcacgccccg cccagatact tcgtcatcga
     1921 cgtcattctt cgcgcctccc cgatctggac gagcgacgag aagcctgaat ccgccattcg
     1981 atttttggct ttcggacgat ggcggattca tcggcgcgaa ccatcgttct cggcagggtg
     2041 agctgttccg cgcaaagcgg ggcaatggag gggccgaaat ttgtggaatg acaatcgcct
     2101 ggcgcgccgg gccgcacatc tttcgacgca gcttcccgct ccccatttcc cgtccgaatt
     2161 cggcgacgcc atcatcctgt tcgattcggg tttcgcggcg ccgcaactgc tccccgatct
     2221 cagccggttc gccgctgagg cgctgaccca ccaccgcgag gaagtgctgc aatatgcgtc
     2281 tggccgcggc cagccctatt tgcgcgaatg gctcgcgaac tggatgaacg aggatggctg
     2341 cgcgctgacc gccgatcata tcctgatcac caacggcgcc aagcagggca tcgaccttat
     2401 ttgccgcctg ctgctcgacg aaggcgacgc gatcgtcgtc accgccccga cctatttcac
     2461 cgccatcccg atcttccgca actatggcgc cgagttcatc gaggtcggac aggatggcgc
     2521 ggggctcgac gtcgccgcgc tcgacgccgc gctcgacgcc cgcgccgcgg ccggcaaggc
//
LOCUS       DATMSU010000489         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_188156,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000489 DATMSU010000000
VERSION     DATMSU010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188156"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VN034_12980"
     CDS             complement(<1..501)
                     /locus_tag="VN034_12980"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038669.1"
                     /note="catalyzes the formation of malate from glyoxylate
                     and acetyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HWV61570.1"
                     /translation="MTEFIDRSGLSVDSRLADFIEQRALPGTGLDAAKFWSDFAGLLG
                     KFAPENAALLAKREDLQARIDAWHKAREGQAHDAAAYQGFLREIGYLVSEPAPFSVGT
                     RNVDPEIATMAGPQLVVPALNARFALNAANARWGSLYDAFYGTDALDAPPAKPGGYDA
                     ERGAAVI"
     gene            659..958
                     /locus_tag="VN034_12985"
     CDS             659..958
                     /locus_tag="VN034_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61571.1"
                     /translation="MDDTRLKLMEAIARKKLVTAQYNGQTLTLAPHLLFERRGDLFIS
                     ALNLNKSWRSDEEPRLGHFKLGGLASIELSEEGFEPLPGFEAAPPREEDTPLLAV"
     gene            complement(963..2105)
                     /locus_tag="VN034_12990"
     CDS             complement(963..2105)
                     /locus_tag="VN034_12990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWV61572.1"
                     /translation="MSVAGIFAVAFAVVAATNRTARGAGWLALGYGMGILYVGLEFLL
                     TRQVDPTPVGIGIFLVFMLALSFALVGVSHHYRVRPPLAAMAVIWVLTILAVPVIFSM
                     TYGSILRLTLYQLPYFAMQSLFGLVIWRSGRRQALDLLLIALQAVAALIYVAKPFIAA
                     TIGTASSPQGYMATTYAAISQSGSVVTLMAIALVMLLVIMRDTTAEMVARSETDPLSS
                     VLNRRGFEHHAEMAFLRGGAAVLITADLDDFKKINDSFGHAAGDGVIAHFAAMLADAA
                     PANAIVGRIGGEEFAVLLPDALLSDGRLYAETVRAAFASVRLPVLGVDRRFTASFGVA
                     QRTSDRSLFELARRADAALYRAKAAGRNQVRVALGEMPPVPAVGVA"
     gene            2327..>2956
                     /locus_tag="VN034_12995"
     CDS             2327..>2956
                     /locus_tag="VN034_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWV61573.1"
                     /translation="MTSLTATENAAIEHAADAPMLSQVEQWAAINSGTGNLAGLKSVA
                     GALADAFSTLPGEVRLVAADPVESVDAAGKVNIIQRGDHLHLRVRPEAPVQLLLTGHM
                     DTVFAADHPFQTLKWLEPGVLNGPGTADMKAGISVILAALAGLEASPLAARVGYDVMI
                     NSDEETGSQASARLIAELAKGKTAALTYEPALPDGTLAGARPGSGNFSVI"
BASE COUNT          579 a          959 c          973 g          445 t
ORIGIN      
        1 gatcaccgcc gcgccgcgct ccgcatcgta gccgccgggc ttcgcgggcg gcgcgtcgag
       61 cgcgtcggtg ccgtaaaacg cgtcgtaaag gctgccccag cgcgcattgg cggcgttgag
      121 cgcgaagcgg gcgttgagtg cgggcacgac gagttgcggc cccgccatcg tcgcgatttc
      181 gggatcgaca ttgcgggtgc cgacgctgaa cggcgcgggt tcggagacga gatagccgat
      241 ctcgcgcaag aaaccctgat aggcagcggc atcgtgcgcc tgcccttcgc gggccttgtg
      301 ccacgcgtcg atccgtgcct gcaaatcctc gcgcttggcg agcagcgcgg cattttcggg
      361 cgcgaacttg ccgagcaacc cggcgaaatc gctccagaat ttggcggcgt cgagccccgt
      421 gcccggcagc gcgcgctgtt cgatgaagtc ggccagtcgc gaatcgacgg acaggccgga
      481 gcgatcgata aattcggtca tgcaagcacc ctctggtcgg cgcggctgcg ccagtggacc
      541 cggggaggaa agggccgctg ccctatgccg cgattggttt cgattggcaa tgggcgccgt
      601 gtaggttacg ttgcaacgca caacgaaaaa agacaccctc ccctaatgat ccggatccat
      661 ggacgacaca cgactgaaat tgatggaagc catcgcccgc aagaagctgg tgacggcgca
      721 atataacggc cagacgctga cgctggcgcc gcatctgctg ttcgaacgcc gcggcgacct
      781 gttcatcagc gcgctcaatc tcaataaaag ctggcggtcg gacgaagaac cgcggctggg
      841 gcatttcaag ctcggcggtc ttgcgtcgat cgaactcagc gaagagggct tcgagcccct
      901 cccgggtttc gaggccgcgc cgccgcgcga agaagacacg ccgctgctcg cggtctgaga
      961 gatcaggcga cgcccacggc gggcacgggc ggcatttcgc caagcgcgac gcgaacctgg
     1021 ttccgtcccg cggccttggc ccgataaagc gcggcatcgg cgcggcgcgc cagttcgaac
     1081 agcgaacggt ccgacgtccg ctgggcgacg ccgaaggacg ccgtgaagcg gcgatcgacg
     1141 ccgagcaccg gcaatcgcac cgatgcgaaa gcggcacgca cggtttcggc ataaagccgc
     1201 ccgtcggaca gcaaggcgtc gggaagcagc accgcgaact cctcgccgcc gatgcggccc
     1261 acgatggcat tggcgggcgc cgcatcggcc agcatcgccg cgaaatgcgc gatcacgccg
     1321 tcgcccgccg catggccgaa actgtcgttg atcttcttga aatcgtcgag atcggcggtg
     1381 atcagcaccg ccgccccgcc gcgcagaaac gccatttcgg catgatgttc gaaaccgcgc
     1441 cggttgagca cgctcgacag cggatcggtt tccgaacgcg cgaccatttc cgccgtcgtg
     1501 tcgcgcatga tcacgagcag catgacgagc gcgatcgcca tcagcgtgac gacgctgccg
     1561 ctctgcgaga tggcggcata ggtcgtcgcc atatagcctt gcggcgaact cgccgttccg
     1621 atcgtcgcgg cgatgaaagg cttggcgacg tagataaggg ccgcgaccgc ctgcaacgcg
     1681 atgagcagca ggtcgagcgc ctgacgccgg cccgatcgcc agatcaccag tccgaacagc
     1741 gactgcatcg cgaaataggg aagctgatag agggtgagcc gcagtatcga accataggtc
     1801 atgctgaaaa tcacgggcac ggcgaggatc gtgaggaccc atatcaccgc catcgcggcc
     1861 aagggcggtc gcacgcgata atgatgcgaa acgccgacca gcgcgaagct gagcgcgagc
     1921 atgaagacga ggaaaatgcc gataccgaca ggggtcggat cgacctgccg cgtcagcaga
     1981 aattcgaggc cgacatagag aatccccatg ccgtagccga gcgcgagcca ccccgcgccg
     2041 cgcgcggtgc ggttggtggc tgcgacaacg gcaaaggcga cggcaaagat gccggccacg
     2101 gacatgttaa tgccaaatat aaaggccgca gtcatcgccc cccactcatc atcgtccgtc
     2161 agcgtaaccg ggctgtcccc aactttcggt taatatattg cgattcggga tgattcgcgc
     2221 gcgtgtttac ccgattggcg ggcccgccgc agtgacttac cgtatcgcag cgacaaaata
     2281 gccggtgggg gtgcaaaagc ccctcgccgc ccgctatagc cgcgcgatga ccagcctgac
     2341 cgcaaccgaa aatgccgcga tcgaacacgc cgccgatgcg ccgatgctgt cgcaggtcga
     2401 acaatgggcg gcgatcaaca gcggcacggg caatcttgcg ggactgaaga gcgtcgcggg
     2461 cgcgctggcc gatgcctttt ccactctgcc gggcgaggtc cggctcgtcg cggccgatcc
     2521 ggtcgagagc gtcgatgcag cgggcaaggt caatatcatc cagcgcggcg accatctgca
     2581 ccttcgtgtg cggcccgagg cgcccgtgca gcttctgctc accggacata tggacacggt
     2641 ctttgcggcc gaccatccgt tccagacgct caaatggctc gaacccggcg tgctcaacgg
     2701 ccccggcact gcggacatga aggcgggcat ttcggtgatc cttgccgcgc tcgccggact
     2761 cgaagcctcg ccgctcgcgg cgcgcgtcgg ctatgacgtg atgatcaaca gcgacgagga
     2821 aacggggagc caggcgtcgg cgcggctgat cgccgagctc gcgaaaggca agaccgcggc
     2881 gctgacctac gaacccgcgc tgcccgacgg cacgctcgcc ggcgcgcggc cggggagcgg
     2941 caatttctcg gtgatc
//
LOCUS       DATMSU010000490         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_26962, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000490 DATMSU010000000
VERSION     DATMSU010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26962"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VN034_13000"
     CDS             <1..730
                     /locus_tag="VN034_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61574.1"
                     /translation="VPAPAPAPAPETPKNLERGDLIALAQRAADAFASGETMPKAVAD
                     AAGRPFELLLPFGCGGPSEAESSLPMQWRYDEAEGTLRVSVQPMMWRNADWNLEDRAD
                     AGAAEGFWITRPWSSSTVCPARAGQAFPRGVEPLTLPGQTLAIAQFFGGEGDRDGRRN
                     GRPFETVTRVAADKFDGSRGFRLRIAGRLEAIAGNAPVRCVQPAGAEQRPICVIGARI
                     DTVRLENPTSEDVLATWTIGRSAQ"
     gene            complement(854..940)
                     /locus_tag="VN034_13005"
     tRNA            complement(854..940)
                     /locus_tag="VN034_13005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(904..906),aa:Leu,seq:gag)
     gene            1247..2821
                     /locus_tag="VN034_13010"
     CDS             1247..2821
                     /locus_tag="VN034_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61575.1"
                     /translation="MAAFARIRLVMLSGVACSILAACGADGVASPGEGVIVIPTPTPT
                     PTPTPTPTPTPTGVTPADACPTINGADQLADLGTISGPQGTWRNCGFPAKFNSTTAIP
                     KVDGVIYSLPGRVDVGSDQGPTEEAGDAADVTLTIDPGVTIFASTGTAFLAVNRGNKI
                     DAVGTPTQPIVFTGRGNVVGSATDNTSQLWGGVVLLGRAPITDCDAAGAVPGTNDCER
                     DTEGASNALYGGEIEDDDSGRMSYVQIRYSGFNLSPDSELQGLTPSGVGSATQFDHIQ
                     MHNSSDDGMEVFGGRPNFKHMIVTGAEDDSFDTDVGYKGNIQYAIAVQRAGGAVGDSI
                     MEIDSDGDENAIPRQEVNISNFTFIHRNAAAGNGAAIRIRGGADYTFANGLITTGASC
                     IRVDSATTVQPADPALDENGPPVFESVSMQCNDRPFRGSNGPDDAAVRAIFELGTSNS
                     STYTPSLTDVFVNGATETALAPFDASALDSFFDVTAYVGAVADANDTWYAGWTCNSAT
                     ASFGDTSGNCTDIPVS"
BASE COUNT          510 a         1061 c          946 g          475 t
ORIGIN      
        1 cgttccggcg cccgctccgg cacctgcccc cgaaacgccc aagaacctcg aacgcggcga
       61 cctcatcgcg ctggcgcagc gcgccgcgga tgccttcgcg tcgggtgaga cgatgccgaa
      121 ggcggttgcg gacgcggccg ggcggccgtt cgaactgctg ctgccgttcg gatgcggggg
      181 gccgagcgag gccgaaagca gcctgccgat gcaatggcgc tatgacgaag ccgaggggac
      241 gctgcgcgtc tcggtccagc cgatgatgtg gaggaacgcc gactggaatc tcgaagaccg
      301 ggcggacgcc ggcgccgccg aaggcttctg gattacgcgg ccctggtcga gcagcaccgt
      361 ttgtccggcg cgcgcgggac aagccttccc gcgcggcgtc gagccgctca ccttacccgg
      421 tcagacgctc gccatcgccc aattcttcgg gggcgagggg gatcgcgacg gtcgccgcaa
      481 cgggcgcccg ttcgaaaccg tgacgcgggt cgccgccgat aaattcgacg gttcgcgggg
      541 tttccgcttg cgtattgcgg gccggctcga agccattgcc ggaaatgcgc cggtccgctg
      601 cgtccagccc gccggggccg aacagcggcc gatctgcgtc atcggcgccc ggatcgacac
      661 cgtccggctc gaaaacccga cgagcgagga tgttctcgcg acatggacga tcggacgttc
      721 ggcccaataa tattctggcg cgaccggcgg tacctggccg ggacgccaga tgggcaacag
      781 gctaccggcg cacggatttc tccatgtgcc gggtcatgcc aggactttga aaatccttgt
      841 tttcaaggaa agatggtgcg gtcgagaaga ctcgaacttc cacggccttt cggccacaac
      901 gacctcaacg ttgcgcgtct accagttccg ccacgaccgc acatcatgat ggtccggatc
      961 gggtccggca ccaggtagga gcgggcgcct agcaaagctt ttcgagcgat gcaagcgagc
     1021 ttcgccgctt ttatttttgc gggtgcgaac gagcgcaccc ccgcgcggcc gtcgctgtca
     1081 ccaaatcgac tccaaagagt cactcaactg tcacggctct ggcgtcagac cgtcatcgaa
     1141 tcaccctagt ggcggcgtca ccgggggcga gtcagcaatt cttcttctcg cccgcggcgt
     1201 gtttttcatc aggggcgcgc aggcgcacac cgggacggag attttcatgg cggctttcgc
     1261 acgcattcgt ttggtcatgc tcagcggcgt ggcctgttcc attcttgcgg cgtgcggcgc
     1321 cgacggcgtc gcgtcgccgg gcgagggcgt gatcgtcatt ccgaccccga ccccgacgcc
     1381 cactccgacc ccgacgccga cccccacgcc gaccggtgtg acccccgccg acgcctgccc
     1441 gaccatcaac ggcgccgacc agctcgccga ccttggcacg attagcggtc cgcagggcac
     1501 ctggcgcaac tgcggcttcc ccgcgaagtt caactcgacg accgcgatcc ccaaggtcga
     1561 cggcgtgatc tactcgctgc ccggccgcgt cgatgtcggc agcgaccagg gtccgaccga
     1621 ggaagccggc gacgccgccg acgtcacgct gacgatcgat ccgggcgtca ccatcttcgc
     1681 ctcgacgggc accgccttcc tcgccgtgaa ccgcggcaac aagatcgacg ccgtcggcac
     1741 gccgacgcag ccgatcgtct tcaccggccg cggcaacgtc gtcggctcgg cgaccgacaa
     1801 cacctcgcag ctgtggggcg gggtcgtcct gctcggccgc gcgccgatca ccgattgcga
     1861 cgcggcgggc gccgttcccg gcaccaacga ttgcgaacgc gacaccgaag gcgcgtcgaa
     1921 cgcgctctat ggcggcgaaa tcgaggacga tgactcgggc cgcatgagct acgtccagat
     1981 tcgctactcg ggcttcaacc tgtcgcccga cagcgagttg caggggctga ccccgtcggg
     2041 tgtcggttcg gcgacccagt tcgaccatat ccagatgcac aacagctcgg acgacggcat
     2101 ggaagtcttc ggcggccgcc cgaacttcaa gcacatgatc gtcaccggcg ccgaggacga
     2161 cagcttcgac accgacgtcg gctacaaggg caatatccaa tatgcgatcg cggtgcagcg
     2221 cgccggcggc gcggtcggcg actcgatcat ggaaatcgac tcggacggcg atgaaaacgc
     2281 gatcccgcgt caggaagtga atatctcgaa tttcaccttc atccaccgca acgccgcggc
     2341 cggcaacggc gccgcgatcc gcatccgcgg cggcgccgac tacaccttcg ccaacggtct
     2401 gatcaccacc ggcgcatcgt gcatccgcgt cgacagcgcg accacggtcc agcccgccga
     2461 cccggcgctc gacgaaaacg gcccgccggt gttcgagtcg gtgtcgatgc agtgcaacga
     2521 tcgtccgttc cgcggctcga acggccccga cgacgccgcg gtccgtgcga tcttcgaact
     2581 cggcacgagc aacagctcga cctacacgcc gagcctgacc gacgtgttcg tcaacggcgc
     2641 gaccgaaacc gcgctggcgc cgttcgacgc ctcggcgctc gacagcttct tcgacgtgac
     2701 ggcctatgtc ggcgcggtcg ccgacgccaa cgacacctgg tacgcgggct ggacctgcaa
     2761 ttccgccacg gcgtcgttcg gcgacaccag cggcaactgt accgacatcc ccgtaagctg
     2821 acgcgccgac gtccgatacc ggggcggggc gcaccactgg cgcgccccgc ccccatttcc
     2881 atctgccatt agggcccatc tccattaggg gttattcaac atgaccaagc ggtcgctatt
     2941 cgccagcttc ctgctccttt cgtcggcgct ggtgtcgccc gcggcgctcg cc
//
LOCUS       DATMSU010000491         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_188179,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000491 DATMSU010000000
VERSION     DATMSU010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188179"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VN034_13015"
     CDS             <1..276
                     /locus_tag="VN034_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWV61576.1"
                     /translation="LFSAAVAARDGAPVWRAIGEAKLWMRPLSGAFIADYVAQNWDSI
                     RWTVGCYEIEGAGVQLFDRVEGDPWTIIGMPMLPLLAWLRATGLAPQ"
     gene            273..1085
                     /gene="aroE"
                     /locus_tag="VN034_13020"
     CDS             273..1085
                     /gene="aroE"
                     /locus_tag="VN034_13020"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWV61577.1"
                     /translation="MSAPYAEVIGDPIAQSKSPLIHGFWLDALGIPGDYRRAHVKPDE
                     LAGYIAERRADPDWRGCNVTMPHKGAVMDLVDDPGDIRGTIGAMNTIVRQPDGSLIGT
                     NTDAAGFYAPLAELDLEGAPVAVVGAGGAARAVLFALARAHVGKVTILNRSPLKAMGL
                     LATFGLKGEVVGLDAPLPPVSLLVNSSSLGMAGQPPLDLDLSPLPDDAIVYDVVYSPL
                     ETGLLKAAAARGLDIVDGLDMLIGQAALAFELFFGASPPEGRDEELRALLTE"
     gene            1082..1705
                     /gene="coaE"
                     /locus_tag="VN034_13025"
     CDS             1082..1705
                     /gene="coaE"
                     /locus_tag="VN034_13025"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543108.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWV61578.1"
                     /translation="MTHRPRPGSRLRRPFILGLTGSIGMGKSTAATMFEREGVPVFDA
                     DAEVHRLQGPGGALVAAIEARFPGTTGPKGVDRQKLGARVLGNTHELAALEAIVHPAV
                     GKAQRRFLARHRARDLVVLDIPLLFEKGGWRKVGAIAVVSAPAWMQRKRVMRRPGMTA
                     AKLKAIRRLQVPDRVKRSRADFIIETGRPKSETHRQIRFIASCFHAR"
     gene            1725..2426
                     /gene="dnaQ"
                     /locus_tag="VN034_13030"
     CDS             1725..2426
                     /gene="dnaQ"
                     /locus_tag="VN034_13030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HWV61579.1"
                     /translation="MREIIFDTETTGFDPRSGDRLVEIGCVELVDRRETGVTFHAYFN
                     PERDMPAAAEAVHGLSIQFLSDKPLFATRVDELIEFLGDAPLIAHNAAFDFGFVNAEL
                     ARAGRPALDMARMCCTVQMARKLHPGAKHSLDALCTRYGIDRSHRVKHGALLDAELLA
                     HLYIEMTGGRQIGLGLAAAAAASVSIDVAAPAAPRRPDRPFREPRPHMASAAELARHA
                     EFVAGLNQPLWLDTV"
     gene            2471..3043
                     /gene="raiA"
                     /locus_tag="VN034_13035"
     CDS             2471..3043
                     /gene="raiA"
                     /locus_tag="VN034_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWV61580.1"
                     /translation="MEIRVSGHQIETGEALQAHVSDRMNAIADKYFSRAIGAHATFGK
                     GPHDSFQCDIVAHVMQGLVLKGHGQAQDAHVAFEGAAERIEKQLRRYMRRLKDHNNGV
                     AAPSPTIDEIEDNAAYTVFDAGSEEDAGDAPAIIAETRVDIPSSSVSDAVMMLDLRNT
                     NALLFVNSRTGTHNMVYRRDDGTIGWVEPR"
     gene            3192..3656
                     /locus_tag="VN034_13040"
     CDS             3192..3656
                     /locus_tag="VN034_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWV61581.1"
                     /translation="MNLSSLLYPATVRAHVQLDSKKALFPFVGDLASRSLGLDAGEVS
                     EALLERERLGSTGFGRGIALPHAKMADLGGVRGLFLQLARPIDFNAVDGLPVDLLFIL
                     LSPLDAGADHLKALAGVSRMLRNEAIADRLRGAKNDEALYALLADTETRDAA"
     gene            3783..4121
                     /locus_tag="VN034_13045"
     CDS             3783..4121
                     /locus_tag="VN034_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HWV61582.1"
                     /translation="MTRLKSRFLVDLLIRRAEAAGGFAAVLAKGDDTSGIILVQCSDR
                     GEPGPLLERRFGPDGHYIWEDVGPTDAKDGESRANYQDRRRRADPDLWLIELDIAEAP
                     QLVAEWAALT"
     gene            4315..4980
                     /locus_tag="VN034_13050"
     CDS             4315..4980
                     /locus_tag="VN034_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HWV61583.1"
                     /translation="MSRILNVAGMAAVGMTAASMLLLAEPGFASDLAADANLPAIILP
                     GADAPAVEEKADLPTEAEAPVEGSNEIETEADSASTPAPVTADSLAELVAATPRPAEI
                     DPELRCLAGAVYFESRGESLPGQLAVAHVVINRAQSGRFPKSLCGVVLQKSQFSFVRG
                     GRMPAIRKGAQWNNAVAIAQIARDGSWKNHAPGALFFHARYVSPGWRKTRIAQIDNHI
                     FYR"
BASE COUNT          869 a         1816 c         1653 g          874 t
ORIGIN      
        1 ctgttcagcg ccgcggtcgc cgcgcgcgac ggcgccccgg tctggcgtgc gatcggcgag
       61 gcgaagctct ggatgcgccc cttgtcgggc gccttcatcg ccgactatgt cgcgcaaaac
      121 tgggacagca tccgctggac cgtcggctgc tatgaaatcg aaggagcggg cgtgcaattg
      181 ttcgaccggg tcgagggcga cccctggacc atcatcggca tgccgatgct ccccttgctg
      241 gcgtggctgc gggccacagg gttagccccg caatgagcgc cccttatgca gaagtgatcg
      301 gcgacccgat cgcccagtcg aagtcgccgc tgatccacgg cttctggctc gacgcgctgg
      361 gcattccggg cgactaccgc cgcgcgcatg tgaagcccga cgagcttgcc ggctatatcg
      421 ccgagcgccg cgccgatccc gactggcgcg gctgcaacgt caccatgccg cacaagggcg
      481 cggtgatgga tctcgtcgac gatcccggcg acatccgcgg cacaatcggc gcgatgaaca
      541 cgatcgtgcg ccagcccgac ggatcgctga tcggtaccaa caccgatgcc gccggcttct
      601 acgccccgct cgccgaactc gatctcgagg gcgcgcccgt cgcggtcgtc ggcgcgggcg
      661 gagcagcgcg cgcggtgctc tttgcgctcg cgcgtgcgca tgtcggcaag gtgacgatcc
      721 tcaaccggtc gccgctcaag gcgatgggtc tgctcgccac tttcgggctc aagggcgagg
      781 tcgtcggcct cgacgctccg ctgccgccgg tgtcgctgct cgtcaattcg agcagcctcg
      841 gcatggcggg ccagccgccg ctcgatctcg acctgtcgcc gctccccgac gatgcgatcg
      901 tctacgacgt cgtctattcg ccgctcgaga cggggctgct caaagccgcc gcggcgcgcg
      961 ggctcgatat cgtcgacggg ctcgacatgc tgatcggcca ggccgcgctc gcctttgagc
     1021 tgttctttgg cgcgtcgccg cccgagggcc gcgacgagga acttcgcgcg ctgctgaccg
     1081 aatgacccac cgcccgcgcc ccggctcgcg gctgcgccgg cctttcatcc tcgggctcac
     1141 cggctcgatc ggcatgggca agtcgaccgc tgcgacgatg ttcgaacgcg aaggcgtccc
     1201 cgttttcgac gccgatgccg aagtgcaccg attgcaaggc cccggcggcg cgctcgtcgc
     1261 ggcgatcgag gcgcgctttc ccggcaccac cggtccaaag ggcgtcgacc gccagaagct
     1321 cggcgcgcgc gtgctcggca atacccacga actcgcggca ttggaggcga tcgtccatcc
     1381 cgcggtgggc aaggcgcaaa ggcgcttcct cgcgcgccac cgcgcacgcg acctcgtcgt
     1441 gctcgacatc cccttgctct tcgaaaaggg cggctggcgc aaggtcggcg cgatcgctgt
     1501 cgtctcggcg ccggcgtgga tgcagcgaaa gcgcgtgatg cgccgccccg gcatgaccgc
     1561 ggcgaagctg aaggcgatcc gccgcctgca ggttcccgac cgcgtcaagc ggtcgcgcgc
     1621 cgatttcatc atcgaaacgg gccgccccaa aagcgagacg caccgtcaaa ttcgcttcat
     1681 cgcctcttgt ttccacgcgc gatagggtcc agattaaagc ctcgatgcgc gagattattt
     1741 tcgataccga aaccacgggt ttcgatccca gaagcgggga caggctggtc gaaatcggat
     1801 gtgtcgagct ggtcgaccgc cgcgaaaccg gcgtcacctt tcacgcctat ttcaatcccg
     1861 agcgcgacat gcccgccgcc gccgaagcgg tgcacggcct gtcgatccag ttcctctccg
     1921 acaagccgct gttcgcgacg cgcgtcgacg agctgatcga attcctcggc gatgcgccgc
     1981 tgatcgcgca caacgccgcc ttcgacttcg gcttcgtcaa cgccgaactc gctcgcgccg
     2041 gccggcccgc gctcgacatg gcgcgcatgt gctgcaccgt gcagatggcg cgcaagctcc
     2101 atcccggtgc caagcacagc ctcgacgcgc tgtgcacgcg ctacggcatc gaccgcagcc
     2161 accgcgtcaa gcatggcgcg ctgctcgacg ccgaattgct cgcgcatctc tatatcgaaa
     2221 tgaccggcgg gcggcagatc ggcctcggcc tcgcggccgc cgctgccgca tcggtgtcga
     2281 tcgatgtcgc ggcgcccgcc gcaccgcgca ggcccgaccg tccctttcgc gaaccccgtc
     2341 cccatatggc gtcggccgcc gaactcgcgc ggcacgccga atttgtcgca gggctgaacc
     2401 agccgctgtg gctcgatacg gtgtaacgac tctcccattg tcgtcgcacc ggccaagaag
     2461 gagaagaaaa atggaaatcc gcgtctctgg ccaccagatc gaaaccggcg aagcgctgca
     2521 ggcgcatgtg tcggaccgga tgaacgcgat tgccgacaaa tatttctcgc gcgcgatcgg
     2581 ggcgcacgcg actttcggaa aaggcccgca cgacagtttc cagtgcgaca tcgtcgcgca
     2641 tgtgatgcag gggctcgtgc tgaaaggcca cggccaggcg caggacgcgc atgtcgcgtt
     2701 cgagggcgcg gccgaacgca tcgaaaagca gctcaggcgc tatatgcggc ggttgaagga
     2761 tcataataac ggcgtggcgg cgccgtcgcc gacgatcgac gagatcgagg acaatgcggc
     2821 ctacaccgtg ttcgatgccg gcagcgaaga ggatgcgggc gacgcgcccg cgatcatcgc
     2881 cgaaacgcgc gtcgacattc ccagcagcag cgtgtccgac gcggtgatga tgcttgacct
     2941 tcggaacacc aacgcgctgc tcttcgtcaa cagcaggacc ggcacgcata acatggtcta
     3001 tcgccgcgac gacgggacga tcggctgggt cgaaccacga tagggctgga ttttctccgc
     3061 catccggctt aagggccgcg cccaacgatg cccgaagccg ggatggcggg gggcctgcca
     3121 cgacagatcc gcgaccgctt gccagcatag ttcctggcgg cggccggcgt ttaacaagat
     3181 ttggccagac gatgaatctt tcttcccttc tctatccggc gaccgtgcgt gcgcatgttc
     3241 agctcgattc gaaaaaggcg cttttcccct tcgtcggcga tctcgccagc cgttcgctcg
     3301 gtctcgacgc cggcgaagtc agcgaagcgc tgctcgaacg cgaacgtctg ggctcaacgg
     3361 gcttcggccg cggcatcgcg ctgccgcacg ccaagatggc cgacctcggc ggggtgcgcg
     3421 gcctgtttct gcaactcgcg aggccgatcg atttcaacgc ggtcgacggg cttccggtcg
     3481 acctgctctt catcctgctc tcgccgctcg acgccggcgc cgaccatctc aaagcgctcg
     3541 cgggcgtgtc gcggatgctg cgcaacgaag cgatcgccga ccggctgcgc ggcgcgaaga
     3601 atgacgaggc gctctacgcg ctgctcgccg acaccgaaac gcgtgacgcg gcctgagaca
     3661 agctcagccg tgcccctgcg aaggcagggg cccattacct gccgtcgcga tttcaagttt
     3721 cccccacctc aggccgccag gttcgatgat gggcccccgc cttcgcaggg gcacaagctg
     3781 acatgacccg cctcaaaagt cgcttcctcg tcgacctgtt gatccgccgc gccgaagcgg
     3841 ccggcggctt tgcggccgtg ctcgccaaag gcgacgacac ctcgggcatt atcctcgtcc
     3901 agtgcagcga ccgcggcgag cccggccccc tcctcgaacg gcgcttcggg cctgacggcc
     3961 actatatctg ggaggacgtc ggcccgaccg acgcaaaaga tggtgaatcg cgcgccaact
     4021 atcaggatcg gcgacgaagg gccgatcccg atctgtggct gattgaactg gatatcgcag
     4081 aagcgccaca actcgtcgcg gagtgggcgg cgttaacttg actctgttgc ggcgcacaat
     4141 agctggcgcc cattcgataa cgcgtaggtc gttccgcggg ttgcacgctt ttgcaaagcg
     4201 cccggcaaaa cggttcggac acgcagtcgg acgatgaccg caggcgatat tggaccatag
     4261 tcccccgcct gttttgagag gttcggttca tcggccgccc ttttgacgga cactatgagt
     4321 cgcattttga acgtggccgg catggctgcc gtcggcatga ctgccgcctc catgctgctt
     4381 ctggcggaac cgggcttcgc aagcgatctt gccgccgacg ccaatcttcc cgcgatcatc
     4441 ctcccgggcg ccgatgcgcc ggcggtcgag gaaaaggcgg atcttccgac cgaagccgag
     4501 gcgccggtcg aaggcagcaa tgaaatcgaa accgaagccg attccgcgtc gaccccggcg
     4561 ccggtgaccg ccgattcgct cgccgaactc gtcgccgcaa cgccgcggcc cgccgaaatc
     4621 gatcccgagc tgcgctgcct cgccggcgcg gtctatttcg aatcgcgcgg cgaatcgctc
     4681 cccggccagc ttgcggtcgc gcatgtcgtg atcaatcgcg cccagtcggg ccgcttcccc
     4741 aagagcctgt gcggcgtcgt ccttcagaaa agccagttca gcttcgtgcg cggtggccgg
     4801 atgccggcga tccgcaaggg cgcgcagtgg aacaacgccg ttgcgatcgc ccagatcgcg
     4861 cgcgacggca gctggaagaa ccacgccccc ggcgcgctct tcttccacgc gcgctacgtc
     4921 tcgccgggct ggcgcaagac gcgcatcgcg caaatcgaca accacatctt ctatcgttaa
     4981 tcgtccgggc ggggtgttca ggcatcccgt tcattgccaa gccatattta ctggcgcatg
     5041 atgccgggat gacgcgtcat gtttctgccg ccctgctcgc tgcagcggcg ctcgctctcg
     5101 gcggctgtgc caccgcggtc ccgccggtcg aagtcacgcg ctttcatacc aacgcggcgc
     5161 caagctgggc gccgggcacg cgctacgcca ttgcgaccgc gccgctcgat gc
//
LOCUS       DATMSU010000492        14915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_168889,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000492 DATMSU010000000
VERSION     DATMSU010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 14915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14915
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168889"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VN034_13055"
     CDS             complement(<1..1446)
                     /locus_tag="VN034_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWV61584.1"
                     /translation="MITSLLIANRGEIACRIIRTAREMGIRTVAVYSDADAKALHVRE
                     ADEAVRIGPSPARESYLIGEKIIAAAKATGAEAIHPGYGFLSENAEFAQAVADAGLVW
                     VGPKPSSITAMGLKDAAKKLMADAGVPVTPGYMGENQDPAFLAEQAAQIGYPVLIKAV
                     AGGGGKGMRKVDAAGDFADALASCQREAAASFGNDHVLIEKYILTPRHIEVQVFGDTH
                     GNVVHLFERDCSLQRRHQKVIEEAPAPGMNEATRAELCAAAVRAAKAVDYVGAGTIEF
                     IADASEGLRADRIWFMEMNTRLQVEHPVTEEITGVDLVEWQLRVASGEPIPLKQDELA
                     INGWAMEARLYAEDPAKGFLPSIGTLELFQLPEHGGRVDTGVYEGAEVSPFYDPMIAK
                     VIAWGEDREEARELLSEMLEDSAIWPVKTNSAFLINALGHPDFVAGTVDTGLIGRDGD
                     AMTEEPVPTAQALTNAAMAMVPRALQSGFRLN"
     gene            complement(1443..2282)
                     /locus_tag="VN034_13060"
     CDS             complement(1443..2282)
                     /locus_tag="VN034_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61585.1"
                     /translation="MAFQPLGYRFEISSTLSLEKAKSRIRQKKKGWFDPQNGARGWIV
                     GPFMCLWLSAFNQHGPMVVARLKHDGMETKIVGRAGADLNGTALFLFLTPLMAWLVYQ
                     MARHGQGSTGQYIILGLLFGAGLPLTLWVNAKDRREADPLVHFVRKALSSTDRSARSL
                     EPSSLAQSSSVRLIVNGEDRAAVSADRLSEALSALSIGDFAVLEFAPDEYMQALSNEN
                     HYIVEKREGSAERHFRAILPKGAIGEHQKYEASLIQLRNVMTAYLHRQTLSSDLNWKR
                     ITL"
     gene            complement(2289..3887)
                     /locus_tag="VN034_13065"
     CDS             complement(2289..3887)
                     /locus_tag="VN034_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWV61586.1"
                     /translation="MSAPVLGTMVNSESDAFRANAAHNRALRDELYARVAEAGLGGNE
                     KSRERHTSRGKLLPRERVERLLDPGSPFLEIGQLAACDMYGGEIPGAGMIAGIGRVSG
                     RQTMIVCNDATVKGGTYYPMTVKKHLRAQEIAEANRLPCIYLVDSGGANLPHQDQVFP
                     DRDHFGRIFFNQANMSAKRIPQIACVMGSCTAGGAYVPAMSDETVIVRNQGTIFLAGP
                     PLVKAATGEEISAEDLGGGDLHAKKSGVVDHLAENDEHALTIVRDIVSHLGADEGFKV
                     EMKEPRPPKYDAEELYGVIPQDVRAPYDVHEVIARIVDASEFHEFKANYGSTLVCGFA
                     HIWGIPVAILANNGVLFSESAVKGAHFIELAQQRRIPLLFLQNISGFMVGGKYEAEGI
                     AKHGAKLVTAVATATVPKITVLIGGSFGAGNYGMCGRAYSPRFLFTWPNARISVMGGE
                     QAASVLATVHRDADKWTPEEAEAFKAPIRQKYEDEGNPYHATARLWDDGIIDPAQTRD
                     VLGLAFAATLNAPVEDRGFGVFRM"
     gene            4034..6127
                     /locus_tag="VN034_13070"
     CDS             4034..6127
                     /locus_tag="VN034_13070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HWV61587.1"
                     /translation="MKKAAFAILLSTAAIVAGPVACSKGDETEKADAAYTVGTELGIS
                     KAAMDTSVKPGDDFYAYANGNWQRTTEIPADRSSIGAFYIAFLETEKRVRDLVGAIVQ
                     KGGEPGTDEGRIAAFYKAYMNRPAIDAAGIRPVAADLARFAAITDKAALSKVLGEQTR
                     ADVDPLNATDFGTENLFGIFVTQGLATPGEVIPYMLQGGLGLPEREYYLSADPKMAAI
                     RTDYQAYIEKLLTAAGQSDAAAKAKRIYDLELKIARAHASREQSEDFTQSADVWTKAD
                     FAKKAPGIDWDAYFAAAKLDKAGKFGAYHANAITKLSALLASEPLDAWKDWLTFHQIN
                     SHSDVLPSAIDNAHFAFYGTTLAGTPQQRSRDKRALDALNTDLGDAVGRVYAEKYFPA
                     SAKAEVGDMVKGIKAAFATRVNGIDWMAPETKKEAIKKVETIVVGVGYPENWRDYGSY
                     SVSATDAYANEVAGGKAEYAHQLAKIGKPMDKAEWWMTPQTVNAVNLPVQNALNFPAA
                     ILQPPFFNAKADPAYNYGAIGAVIGHEISHSFDNNGAAFDSTGALRNWWTAADLKKFE
                     EAGSALAAQYDTYKPFPDLAVNGKLTLGENIADVAGLQAAYDAYRASLNGKEAPVIGG
                     FTGDQRFFIAYAQTWATKMRDEALRARVATDGHAPGNYRALTVRNLDAWYKAFDVKEG
                     DKLYLAPDKRVRVWG"
     gene            complement(6273..6560)
                     /locus_tag="VN034_13075"
     CDS             complement(6273..6560)
                     /locus_tag="VN034_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61588.1"
                     /translation="MSQALLDVLQYYGAGAATLAALLVSLNLGRRWTGWAFVIFVTSS
                     LALIGWGFLQPDSEGIGWQNIALLIINCVGVYRYLILKEKPGAPVEEADGQ"
     gene            complement(6557..6949)
                     /locus_tag="VN034_13080"
     CDS             complement(6557..6949)
                     /locus_tag="VN034_13080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61589.1"
                     /translation="MTEFRVTGFDHIVLCVRDVAATRAFYERVLGMAAREERPGKYSL
                     QFGENKISLQDAANSPTIARDTVPGSGNFCLLSDTPVAEWRAHLAAEGVEIIDSGLRD
                     GATGTIDSLYFRDPDGNLVEVSNRVDRR"
     gene            complement(6946..7365)
                     /locus_tag="VN034_13085"
     CDS             complement(6946..7365)
                     /locus_tag="VN034_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61590.1"
                     /translation="MTMRGLVHHIDLTVTDKERSRPFYDAVLGFLGYRRSADDDRGSD
                     WDREGEPFHSIGIVEAHGQGASRKHDRTSPGLHHLAWAAESREDVDALHDLLRGIGAT
                     ILDAPADYPRYGPTYYAVFFADPDGLKLEFVFGATGA"
     gene            complement(7362..7712)
                     /locus_tag="VN034_13090"
     CDS             complement(7362..7712)
                     /locus_tag="VN034_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61591.1"
                     /translation="MIWLWLSAAFMVTTAGVHSVLGYRRMIVPLLRGEGGPLADALTR
                     RIVRFAWHATSVLMLISAAAVAWPGTPKGVLIVIGGGWLASGLFDAAYTKGRHIAWPA
                     LAGAGILALIGALA"
     gene            complement(7709..8878)
                     /locus_tag="VN034_13095"
     CDS             complement(7709..8878)
                     /locus_tag="VN034_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HWV61592.1"
                     /translation="MRATPDFDFALGETAEMIRETTARFADEQIAPLAAKADAQDWFP
                     RDELWTAMGELGLHGITVEEEFGGLGLGYLEHVIAVEEVSRASAAIGLSYGAHSNLCV
                     NQIRRWGNAEQKAKYLPKLISGEHVGSLAMSEAGAGSDVVSMKLKADKVQGGYVLNGT
                     KFWITNATHADTLVVYAKTSPEAGSRGITAFLIEKDMPGFSIGQKIDKVGMRGSPTAE
                     LVFTDCEVSEEQVMGPENGGVGVLMSGLDYERVVLAGLQLGIMQACLDTVIPYVRERK
                     QFGKPIGSFQLMQAKVADMYVMLQSARSYVYNVAKACDAGQTTRFDAAGCILLASENA
                     VKVAGEAIQALGGAGYTKDWPVERYWRDAKLLDIGAGTNEIRRMLIGRELIGAGA"
     gene            8979..9881
                     /locus_tag="VN034_13100"
     CDS             8979..9881
                     /locus_tag="VN034_13100"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV61593.1"
                     /translation="MIKRTHIRQFLAVVDAGSFTQAALRIRVTQPALSTGIAELEKLV
                     GTPLFIRNRRQIRLTEAGGRFLPIARDLERGFRAADGFGRDADRQAAELKLGIIRSAP
                     GELLQAIAAALRPGFSIELVENSDSELRSALGSGRLHLALVALRDGERGDHVVPLYEE
                     PLAMFVAADHPLAGRIEVAPEELAAETMIARRSCEFLDATSRFFTRHGVRPRFALRSE
                     SDERCLRMVAAGVGITTAPMSLAIEGIVPLKVAGYDFRRELGLLLAPAWGALPEIAPR
                     LAHALETICAIAAEWRRSRVDGAN"
     gene            10052..10957
                     /gene="cysD"
                     /locus_tag="VN034_13105"
     CDS             10052..10957
                     /gene="cysD"
                     /locus_tag="VN034_13105"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWV61594.1"
                     /translation="MSSELTHLDRLEAESIHIMREVMADATKPVMLYSVGKDSAVMLH
                     LARKAFYPSPPPFPLLHVDTTWKFQAMYELRDRMAAESGMDLIVYQNPEAKARGINPF
                     DHGPLHTDMWKTEGLKQALDLHGFDVAFGGARRDEEKSRAKERIFSFRTASHGWDPKK
                     QRPELWNLYNARKAKGESIRVFPISNWTELDIWQYIARENIPIVPLYFAAPRPTVERD
                     GLLLMVDDDRFPLKDGETPVERSVRFRTLGCYPLTGAVESEASTLSEVIQEMLLTTTS
                     ERQGRIIDKDGGDASMEKKKQEGYF"
     gene            10957..12858
                     /gene="cysN"
                     /locus_tag="VN034_13110"
     CDS             10957..12858
                     /gene="cysN"
                     /locus_tag="VN034_13110"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HWV61595.1"
                     /translation="MTDPVYVTDALIAEDIDAYLAGHEKKSLLRFITCGSVDDGKSTL
                     IGRLLYDSKMIFEDQLAALEADSKRVGTQGQEIDFALLVDGLAAEREQGITIDVAYRF
                     FTTEKRKFIVADTPGHEQYTRNMVTGASTADLAVILIDARKGVLTQTRRHSFLAHLIG
                     IKHIVLAVNKMDLVGYDKTVFDRITLAYRAFASEIGITHFTAIPISGFKGDNITALSD
                     NTPWFKGPALIEHLENVEVGTAADEAKPFRMPVQWVNRPNLDFRGFSGQLASGKVRPG
                     DAVRILPSGKTTKVERIVTLDGDLGEAVAGQSVTLTLTDEVDCSRGDVIAAADAPPEA
                     ADQFEATLVWMADEAMIPGRAYWLKLATQSVSATVQAPKYEINVNTLDHLAAKTLDLN
                     GIGVVELSTDKPITFEAYADNRTLGGFILIDKLTNATVAAGMLHFSLRRAQNVHWQAT
                     DIDRDMRANLKNQRPALLWFTGLSGSGKSTIANLVEKKLHRMNRHSFLLDGDNVRHGL
                     NRDLGFTEADRIENIRRVGEVAKLMSDAGLIVITAFISPFRAEREMVRGMLPEGEFIE
                     VFIDTPLAEAERRDVKGLYKKARAGQLKNFTGIDSPYEAPESPEIRIDTTAMTPEEAA
                     DLIIDRLLG"
     gene            12868..13614
                     /locus_tag="VN034_13115"
     CDS             12868..13614
                     /locus_tag="VN034_13115"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HWV61596.1"
                     /translation="MGESDEQLAERLATAAGRILLDLRAKGELDGKALGKAGDEQANA
                     LLCREIRAARPDDALLSEEEKDNFARCGQSRVWIVDPLDGTREYGEGRDDWAVHVALA
                     IDGVATVGAVALPGLGLTLTSGAPAPLQPANQPLKMLVSRTRPAAEAVFVAEKLGAEL
                     LAMGSAGAKAMAVVRGEADIYLHTGGQYEWDNCAPVAVAQAAGLHVSRVDGSPLRYNN
                     PETYLPDLLICRKELAAEVLRLAAEYSPAD"
     gene            13736..14494
                     /locus_tag="VN034_13120"
     CDS             13736..14494
                     /locus_tag="VN034_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61597.1"
                     /translation="MELEDKTIIVTGASSGIGAAAAKLFASEGANVVLGARRGAELEA
                     LVAAINKTDGRAVFLAGDVRDEGYAGALVDLAGKAFGGLDGAFNNAGIVGEMQPVPDM
                     AIGNWTDVISVNLTAAFLAAKAQIPAMKARGRGSIVFTSSFVGFSNGGMPGMGAYAAS
                     KAGLIGFAQSLASDHAAEGIRVNALLPGGTITPAGGEGNPAALEFIAGLHPMKRMAAP
                     VEIAQAALFLLSDRSSFMTGSPMIADGGISVRLA"
     gene            14656..>14915
                     /locus_tag="VN034_13125"
     CDS             14656..>14915
                     /locus_tag="VN034_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HWV61598.1"
                     /translation="MTDLPETNLTMLTLVKPEGQLEVSLERRPMPVPKPHEVLVKVLA
                     APINPSDLGLLFGGADMSTARASTRDGLPVLTADVPPAGKRA"
BASE COUNT         2726 a         4882 c         4728 g         2579 t
ORIGIN      
        1 gttgaggcgg aagccggact gcaacgcgcg cggcaccatc gccatcgcgg cgttggtgag
       61 tgcctgcgcg gttgggaccg gctcttcggt catcgcgtcg ccgtcgcggc cgatcaggcc
      121 cgtgtcgacg gtgcccgcga cgaaatcggg atgaccgagc gcgttgatca gaaacgccga
      181 gttggtcttc accggccaga tcgcgctgtc ctcgagcatc tcggacagca attcgcgcgc
      241 ttcctcgcga tcctcgcccc aagcgatgac cttggcgatc atcgggtcat agaagggcga
      301 gacctcggcg ccttcataga cgccggtgtc gacgcggccg ccatgttcgg ggagctggaa
      361 gagttcgagc gtgccgatcg agggcaggaa tcccttggca ggatcctcgg cataaagccg
      421 cgcttccatc gcccagccgt tgatcgcgag ctcgtcctgc ttgagcggga tcggctcgcc
      481 cgacgcaacg cggagttgcc attcgacgag gtcgaccccg gtgatttcct cggtcaccgg
      541 atgctcgacc tgcaggcgcg tgttcatttc catgaaccag atgcggtcgg cgcgcaggcc
      601 ctccgacgcg tcggcgataa actcgatcgt tcccgcgccg acatagtcga ccgccttcgc
      661 cgcgcgcacc gcggctgcgc agagttcggc gcgcgtcgcc tcgttcatgc cgggggcggg
      721 ggcttcctcg atcaccttct ggtggcggcg ctggagcgag cagtcgcgct cgaacaggtg
      781 gacgacattg ccgtgcgtgt cgccgaacac ctgcacctcg atatggcgcg gggtgaggat
      841 atatttctcg atgagcacat gatcgttgcc gaacgatgcg gccgcctcgc gctggcacga
      901 ggcgagggca tcggcgaaat cgcccgcggc atcgaccttg cgcatcccct tgccgccgcc
      961 gcccgcgacc gccttgatca gcaccggata gccgatctgt gccgcctgtt cggcgaggaa
     1021 ggccgggtcc tgattttcgc ccatatagcc cggcgtcacc ggcaccccgg catcggccat
     1081 aagcttcttc gcagcatcct tcaggcccat cgcggtgatc gacgacggct tcggtccgac
     1141 ccagacgagg cccgcatcgg cgaccgcctg cgcgaactcg gcgttttcgg agagaaagcc
     1201 atagccggga tggatcgcct cggcgccggt cgccttggcg gcggcgatga tcttctcgcc
     1261 gatcagatag gattcacgcg ccggcgatgg cccgatgcgc accgcctcat cggcctcgcg
     1321 cacatgcagc gccttcgcat cggcatccga ataaaccgcg acggtcctga tgcccatctc
     1381 gcgcgcggtg cggatgatgc ggcaagcgat ttcgccacgg ttggcgatga ggagggaggt
     1441 gatcatagcg ttatgcgctt ccagttaaga tcggagctaa gggtttgccg atgaagatag
     1501 gctgtcatca cattcctgag ttggattaga ctggcctcgt atttttgatg ctcgccgatg
     1561 gctccctttg gcagaattgc ccggaaatgg cgctcggcac tgccttcccg tttctccaca
     1621 atgtaatgat tttcatttga cagggcctgc atatactcgt ccggcgcaaa ctccagaacg
     1681 gcgaagtcgc cgatcgatag cgctgaaaga gcttcgctta atcgatcagc ggatactgcg
     1741 gcgcggtctt caccattcac gatcaggcga acggaactcg attgtgcaag cgatgaaggt
     1801 tctaaactgc gcgcgcttct gtcggtcgat gacagtgcct tgcggacaaa atgcaccagc
     1861 gggtcggctt cccggcggtc cttggcgttt acccaaagtg tcaagggcag accggcgcca
     1921 aaaaggagtc ccaagatgat atactggcct gtcgacccct gcccatgtcg agccatctga
     1981 tagaccaacc atgccatcaa aggggtgaga aacaagaaca gtgcggtgcc attcagatcg
     2041 gccccggctc gccccacgat tttggtttcc atcccgtcgt gtttcagccg cgccacaacc
     2101 atcggcccat gttggttgaa agcgcttagc cacaggcaca taaatggacc gacgatccaa
     2161 cctcgcgccc cgttctgcgg atcgaaccac ccctttttct tctgccgaat cctgctcttc
     2221 gctttttcga gcgaaagcgt tgagctgatt tcaaacctat agccgagagg ctggaacgcc
     2281 atgataattt acatcctgaa cacgccgaat ccgcggtctt cgaccggggc attcagcgtc
     2341 gccgcgaagg ccagccccag cacgtcgcgc gtctgcgcgg ggtcgatgat gccatcgtcc
     2401 caaagccgtg cggtggcgtg atagggattg ccttcgtcct catatttctg gcggatcggc
     2461 gccttaaagg cttcggcttc ctcgggcgtc catttgtcgg cgtcgcggtg gaccgttgcc
     2521 agcaccgacg ccgcctgttc gccgcccatc acgctgatcc gcgcattggg ccaggtgaag
     2581 aggaagcgcg ggctgtatgc gcgcccgcac atgccgtaat tgcccgcgcc gaagctgccg
     2641 ccgatcagca cggtgatctt cggcacggtg gccgtggcta cggctgtcac cagcttcgcg
     2701 ccatgcttgg cgatcccctc ggcttcatat ttgccgccga ccatgaagcc gctgatattc
     2761 tggaggaaga gcaggggaat gcgccgctgc tgcgccagct cgatgaaatg cgcgcccttg
     2821 accgcgctct cgctgaacag cacaccgttg ttcgcgagga tcgcgacggg gatgccccag
     2881 atatgcgcga agccgcagac gagcgtgctg ccgtaattcg ccttgaactc gtggaactcg
     2941 ctggcatcga cgatgcgcgc gatcacctcg tggacatcat agggcgcgcg cacgtcttgc
     3001 gggatgacgc cgtacagttc ttccgcatca tatttgggcg gccgcggctc tttcatctcg
     3061 accttgaaac cttcgtcggc gccgagatga ctgacgatgt cgcgcacgat cgtcagcgca
     3121 tgctcgtcat tttcggcgag atgatcgacg acgcccgatt tctttgcgtg caggtcgccg
     3181 ccgcccaaat cctcggcgct gatttcctca cccgtcgctg ccttgaccag cggcgggcca
     3241 gcgaggaaga tcgtgccctg gttgcgcaca atgacggttt catcgctcat cgcgggcaca
     3301 taggcgccgc ccgcggtgca gcttcccatc acgcacgcga tctgcgggat gcgcttcgcc
     3361 gacatattcg cctgattgaa gaagatgcgc ccgaaatggt cgcggtcggg aaacacctga
     3421 tcctgatgcg gcaggttcgc gccgccggag tcgacgaggt agatgcaggg caggcggttc
     3481 gcctcggcga tctcctgcgc gcgaagatgc ttcttcaccg tcatcggata gtaagtgccg
     3541 cccttcaccg tcgcgtcgtt gcagacgatc atcgtctgcc gccccgacac gcgcccgatg
     3601 cccgcgatca tcccggcgcc cggaatttcg ccgccataca tgtcgcacgc cgcgagctgg
     3661 ccgatctcga ggaagggcga gcccggatcg agcagccgtt cgacacgctc gcgcggcagc
     3721 agcttgccgc ggctcgtgtg gcgttcgcgt gacttttcat tgccgccgag accggcctcg
     3781 gcgacgcgcg cgtaaagctc gtcgcgaagc gcgcgattgt gcgcggcatt ggcgcggaac
     3841 gcgtcgctct cgctattcac catcgtgccc agaacaggtg cgctcacgtc tcgaaatctc
     3901 ctctgaagtc ggcccggact atcccgccgc tcgtcgagcg acaaggaccg caggccgaaa
     3961 atggttggcg ttgaaaccag ttcgtcacag gcctatgccc ctcgcatgcc cgtctaggaa
     4021 caaggtcatt ctcatgaaaa aagccgcttt cgccatattg ctttcgaccg ccgcgatcgt
     4081 cgcggggccc gtcgcctgtt cgaagggcga cgagactgaa aaggccgatg ccgcctacac
     4141 cgtcggcacc gaactcggga tcagcaaggc cgcgatggac acgagcgtga agcccggcga
     4201 cgacttttac gcctatgcca acggcaattg gcagaggacg accgagatcc ccgccgaccg
     4261 ctcgtcgatc ggcgccttct acatcgcctt tctcgaaacc gagaagcgcg tccgcgacct
     4321 cgtcggcgcg atcgtccaga agggcggcga gcccggcacc gacgaaggcc gcatcgccgc
     4381 tttctacaag gcctatatga acaggccggc gatcgacgct gcgggcatca ggccggttgc
     4441 ggccgacctc gcacgcttcg ccgcgatcac cgacaaggcc gcactgtcga aagtgctcgg
     4501 cgaacagacc cgcgccgacg tcgatccctt gaacgccacc gacttcggca ccgaaaatct
     4561 cttcggcatc ttcgtgacgc aggggctcgc gacgcccggt gaggtgatcc cctatatgct
     4621 gcagggcggc ctcgggcttc ccgagcgcga atattatctg tcggccgacc ccaagatggc
     4681 ggcgatccgc accgactatc aggcctatat cgaaaagctg ctcaccgccg cggggcagag
     4741 cgacgccgcg gcgaaggcca agcgcatcta tgacctcgaa ctcaagatcg cgcgcgcgca
     4801 cgccagccgc gaacagagcg aggacttcac ccaatcggcc gacgtctgga ccaaggccga
     4861 tttcgccaag aaagcgccgg gtatcgactg ggacgcttat ttcgctgcgg cgaagctcga
     4921 caaggccggc aagttcggcg cctatcacgc gaacgcgatc acgaagctgt cggcgctttt
     4981 ggcgtcggag ccgctcgatg cgtggaagga ctggctgacg ttccaccaga tcaacagcca
     5041 tagcgacgtg ctgccgagcg cgatcgacaa tgcccacttt gctttttacg gcacgacgct
     5101 tgcgggcaca ccgcagcagc gcagccgcga caagcgcgcg ctcgacgcgc tgaacaccga
     5161 cctcggcgac gcggtcggcc gcgtctatgc cgaaaaatat ttcccggcct ccgccaaagc
     5221 cgaagtcggc gacatggtga aggggatcaa ggcggcgttt gccacccgcg tgaacggcat
     5281 cgactggatg gcgcccgaaa cgaagaagga ggcgatcaag aaggtcgaaa cgatcgtcgt
     5341 cggcgtcggc tatcccgaaa actggcgcga ttatggcagc tacagcgtca gcgcgaccga
     5401 cgcctatgcg aacgaggttg cgggcgggaa ggccgaatat gcgcaccagc tcgccaagat
     5461 cggcaagccg atggacaagg ccgaatggtg gatgaccccg cagacggtca acgcggtcaa
     5521 cctgccggtg cagaacgcgc tgaacttccc cgccgcgatc ctccagccgc ccttcttcaa
     5581 tgccaaggcc gatccggcct ataattatgg cgcgatcggt gcggtgatcg gccacgagat
     5641 cagccacagc ttcgacaata atggcgcggc gttcgactcg accggcgcgc tgcgcaactg
     5701 gtggaccgcc gccgacctca agaagttcga ggaagcgggg tcggcgctgg ccgcgcagta
     5761 cgacacgtac aagccgttcc ccgacctcgc ggtaaacggc aagctgacgc tgggcgagaa
     5821 tatcgccgac gtcgcgggac tgcaggcggc gtatgacgcg tatcgcgcgt cgctcaatgg
     5881 caaggaagcg ccggttatcg gcggcttcac cggcgaccag cgcttcttca tcgcctatgc
     5941 gcagacctgg gcgaccaaga tgcgcgacga ggcgctgcgc gcgcgcgttg ctaccgacgg
     6001 tcatgcgccg ggcaattatc gcgcgctcac cgtccgcaac ctcgacgcct ggtacaaggc
     6061 gttcgatgtc aaggaaggcg acaagctcta cctcgcgccc gacaagcgcg tccgcgtctg
     6121 gggttaagct cgaagctccc ctccccacag gggaggggtt gggggtgggg tgccgcaggc
     6181 gttctttctt cgccgcgata cccaccccac tgcgactagc gagcaagctc gcaagtctcg
     6241 tacccctccc tgaaagggaa gggattaggc cgtcattgcc catccgcttc ctccaccggc
     6301 gcgccgggct tttccttgag gatcagatag cgatagacgc cgacgcagtt gatgatcagc
     6361 aaagcgatat tctgccaccc gatgccttcg ctgtcgggtt gcaggaaccc ccagccgatc
     6421 agcgcgaggc tgctcgtcac gaagatgacg aacgcccagc cggtccagcg ccgcccgagg
     6481 ttgagcgaga cgagcagcgc cgcgagcgtc gccgcgcccg cgccataata ttgcaggacg
     6541 tcgagcagcg cctggctcac cggcgatcga cccggttgct gacctcgacg agattgccgt
     6601 cggggtcgcg gaaatagagc gagtcgatcg tcccggtcgc gccgtcgcga agtccgctgt
     6661 cgatgatctc gactccctcg gccgccagat gtgcgcgcca ttcggcgacc ggcgtgtcgc
     6721 tgagcaggca gaaattgccg ctgccgggca cggtatcgcg tgcgatggtc gggctgttgg
     6781 cggcgtcctg caggctgatc ttgttttcgc caaactgcag cgaatatttg ccgggccgct
     6841 cttcgcgcgc ggccatgccc aaaacacgct cgtagaaggc gcgcgtcgcg gcgacgtcgc
     6901 ggacgcacag cacgatatga tcgaagccgg tgacgcgaaa ttcggtcatg cgcccgtcgc
     6961 tccgaaaaca aactccagct tcagcccgtc gggatcggca aaaaagaccg cataataggt
     7021 cgggccatag cgcggataat cggcgggcgc gtcgaggatc gtcgcgccga tgccgcgcag
     7081 cagatcgtgc agcgcatcga catcctcgcg gctctccgcc gcccaagcga gatgatgcag
     7141 gcccggcgaa gtgcggtcgt gtttccgcga cgcgccctgc ccatgcgcct cgacgatacc
     7201 gatcgaatgg aagggctcgc cctcgcggtc ccaatcgctg ccgcggtcgt cgtcggccga
     7261 acgacgatag ccgaggaagc cgagcacggc gtcgtagaag gggcgcgagc gctccttgtc
     7321 ggtgaccgtc aggtcgatgt ggtggacgag cccgcgcatg gtcatgccaa cgcaccgatc
     7381 aaggcaagga tgcccgcgcc ggcgagcgcc ggccatgcga tatggcgccc cttcgtatag
     7441 gcggcatcga acagcccgga ggcaagccac ccgccgccga tgacgatcaa tacacccttg
     7501 ggtgtgccgg gccatgcgac cgctgccgcc gaaatcagca tcagcaccga tgtcgcatgc
     7561 catgcgaagc ggacgatccg gcgcgtcagc gcgtcggcga gcggcccgcc ttcgccgcgc
     7621 agcaacggca cgatcatccg ccgatagccg agcaccgaat gaaccccggc cgtcgtcacc
     7681 atgaaggcgg ccgacagcca gagccagatc acgcgccggc gccgatcagt tcgcggccga
     7741 tcagcatgcg gcggatctcg ttcgtgcccg cgccgatgtc gagcagcttg gcgtcgcgcc
     7801 aatagcgttc gaccggccag tccttggtat agcccgcgcc gcccaaggcc tgaatcgcct
     7861 cgcccgcaac cttcaccgca ttctcgctcg cgagcaggat gcagcccgcg gcgtcgaagc
     7921 gcgtcgtctg ccccgcgtcg cacgccttgg cgacattata gacataggag cgcgccgact
     7981 ggagcatgac atacatgtcg gcgaccttcg cttgcatcag ctggaacgac ccgatcggct
     8041 tgccgaactg cttgcgctcg cggacatagg gaatgaccgt gtcgaggcac gcctgcataa
     8101 tgccgagctg aagcccggcg agcacgacgc gctcataatc gagccccgac atcagcacgc
     8161 cgacgccgcc attttcgggg cccatcactt gctcttcgga cacttcgcag tcggtgaaga
     8221 cgagttcggc ggtcggcgag ccgcgcatgc cgaccttgtc gatcttctgc ccgatgctga
     8281 agcccggcat gtccttttcg atcagaaagg cggtgatccc gcgcgatccg gcttcggggc
     8341 tggtcttggc atagacgacg agcgtgtcgg catgcgtcgc gttggtgatc cagaatttgg
     8401 tgccgttgag gacatagccg ccctgaacct tgtcggcttt cagcttcatc gacacgacat
     8461 cgctgcccgc gccggcttcg gacatcgcga ggctgccgac atgctcgccc gagattagct
     8521 tggggagata tttggctttc tgctcggcat tgccccagcg gcggatctgg ttgacgcaca
     8581 ggttcgaatg ggcgccatag gagaggccga tcgcggcgct tgcgcggctc acttcctcga
     8641 ccgcgatgac atgctcgaga tagccgagcc cgagcccgcc gaactcttcc tcgacggtga
     8701 tgccgtgcag gccaagctcg cccatcgccg tccacagctc gtcgcgcggg aaccagtcct
     8761 gcgcgtcggc cttggcggcc agcggggcga tctgttcgtc ggcgaagcgg gcggtggttt
     8821 cgcggatcat ctcggcggtt tcgccgagcg cgaagtcgaa atcgggggtg gcgcgcatga
     8881 gggacctgtc gattgggggc gggactgcac gcgatagtgc ctcaaaattt caccataggg
     8941 aaattgaaac gattgcacat ttatcataag taaaatctat gattaagcgc acccatatcc
     9001 gccaatttct tgccgtggtc gacgcgggca gtttcacgca agcggcgctc cgcattcgcg
     9061 tgacccagcc ggcgctgtcg accgggatcg ccgaactcga aaagctcgtc ggcaccccgc
     9121 tcttcatccg caaccgccgc cagatccggc tgaccgaggc gggcggccgc tttctgccga
     9181 tcgcgcgcga cctcgaacgc ggctttcgcg ccgccgacgg cttcgggcgc gacgccgacc
     9241 ggcaggcggc cgaactcaag ctcggcatca tccgctcggc accgggcgaa ttactgcagg
     9301 cgatcgccgc ggccttgcgc ccgggctttt cgatcgagct cgtcgaaaac agcgactccg
     9361 aacttcgctc ggcgctcggc agcggccggc ttcatttggc gcttgtcgcg ctccgcgacg
     9421 gcgagcgcgg cgaccatgtc gtcccgctct acgaggaacc gctcgcgatg ttcgtcgcgg
     9481 cggaccatcc gctcgcgggg cggatcgagg tggcgcccga ggaactcgcc gccgagacga
     9541 tgatcgcgcg ccgctcgtgc gaatttctcg acgcgacgag ccgcttcttc acgcgccacg
     9601 gcgttcgccc gcgcttcgcc ttgcgcagcg aaagcgacga acgctgcctg cgcatggtcg
     9661 ccgccggggt cggtatcacc accgccccga tgtcgctcgc gatcgagggc atcgtcccgc
     9721 tgaaggtcgc gggctatgat ttccgccgcg aactcgggct gcttctcgcc ccggcctggg
     9781 gggcgctacc cgagatcgcg ccgcgccttg cgcacgcgct cgaaaccatc tgtgcgatcg
     9841 cggccgaatg gcgacgatcg cgggtcgacg gagccaactg acgccgtctc ccacaagtct
     9901 ggcttgcagc gcccaaaggc cataacgttt gcccgccaac atcggccgga gctacggcgg
     9961 cctcatccac cccgtccgtt tggacgtatg acggaatttg aaatagccta ttccgctggc
    10021 gcacggcgga agtgtgagga gacgaagttt gttgtcgtcc gagttgacgc atctcgaccg
    10081 gctcgaggcc gagagcatcc atatcatgcg cgaagtgatg gccgatgcca ccaagcccgt
    10141 gatgctctac agtgtcggca aggacagcgc ggtgatgctc catctcgcgc gcaaggcctt
    10201 ttatccctcg ccgccgccgt tcccgctgct ccatgtcgat acgacgtgga agttccaggc
    10261 gatgtatgaa ctccgcgacc gcatggcggc cgaaagcggc atggacctca tcgtctatca
    10321 gaaccccgaa gccaaagcgc gcgggatcaa tcccttcgac cacggcccct tgcacaccga
    10381 catgtggaag acggaggggc tgaagcaggc gctcgacctc cacggtttcg acgtcgcctt
    10441 cgggggcgcg cgccgcgacg aggaaaagag ccgcgccaag gagcgcatct tctccttccg
    10501 taccgcgagc cacggctggg atcccaagaa gcagcgcccc gagctttgga atctctataa
    10561 tgcgcgcaaa gcgaagggcg aaagcatccg cgtcttcccg atctcgaact ggaccgagct
    10621 cgacatctgg caatatatcg cgcgtgagaa tatcccgatc gtgccgctct atttcgccgc
    10681 gccgcgcccg accgtcgagc gcgatggctt gctcttgatg gtcgatgacg accgttttcc
    10741 gctgaaggat ggcgaaacgc ccgtcgaacg ctcggtgcgt ttccgcaccc tcggctgcta
    10801 cccgctcacg ggtgcggtcg agagcgaagc cagcacgctg tccgaagtga tccaggaaat
    10861 gctgctgacc accaccagcg aacggcaagg ccgcatcatc gacaaggatg gcggtgatgc
    10921 cagcatggag aagaagaagc aggaggggta tttctgatga ccgatcctgt ttacgtcacc
    10981 gacgcgctga tcgccgagga catcgacgcc tatctcgcgg ggcatgagaa gaaatcgctc
    11041 ctccgcttca tcacctgcgg ctcggtcgac gacggcaagt cgacgctgat cgggcgcctg
    11101 ctctatgaca gcaagatgat cttcgaggac cagctcgcgg cgctcgaagc cgacagcaag
    11161 cgcgtcggca cacaggggca ggagatcgat ttcgcgctgc tcgtcgacgg cctcgccgcc
    11221 gagcgcgagc aggggatcac gatcgacgtc gcctatcgct ttttcacgac cgagaagcgc
    11281 aagttcatcg tcgccgacac gccggggcat gaacaatata cgcgcaacat ggtcaccggc
    11341 gcctcgaccg ccgaccttgc cgtcatcctg atcgacgcgc gcaagggcgt gctgacccag
    11401 acgcgccgcc acagcttcct cgcgcacctg atcggtatca aacatatcgt tctcgcggtg
    11461 aacaagatgg accttgtcgg ctacgacaag acggtgttcg atcgcatcac gctggcgtac
    11521 cgcgctttcg cgagcgagat cggcatcacc catttcaccg cgatcccgat ctcgggcttc
    11581 aagggcgaca atatcaccgc gctctcggac aatacgccct ggttcaaggg ccccgcgctg
    11641 atcgagcatc tcgaaaatgt cgaggtcggc actgccgccg atgaagcgaa gccgttccgc
    11701 atgccggtcc agtgggtgaa ccgcccgaac ctcgatttcc gcggtttctc gggccagctc
    11761 gcgagcggca aggttaggcc cggcgatgcc gtgcgtatcc tgccgagcgg caagacgacg
    11821 aaggtcgagc gcatcgtcac cctcgacggc gacttgggcg aagccgttgc cggccagtcg
    11881 gtcacgctca ccctcaccga tgaggtcgat tgctcgcgcg gcgacgtcat cgccgccgcc
    11941 gacgcgccgc ccgaggcggc cgaccagttc gaggcgacgc tcgtctggat ggccgacgag
    12001 gcgatgatcc cgggccgcgc ctattggctc aagctcgcga cgcagagcgt ttcggcgacg
    12061 gtgcaggcgc cgaagtatga gatcaacgtc aacacgctcg accatctcgc ggcgaagacg
    12121 ctcgacctca acggcatcgg cgtcgtcgaa ctgtcgaccg acaagccgat cactttcgaa
    12181 gcctatgccg acaaccggac gctcggcggc ttcatcctga tcgacaagct caccaacgcc
    12241 acggtcgcgg cgggcatgct gcacttcagc ctgcgccgcg cgcaaaatgt ccattggcag
    12301 gcgaccgaca tcgaccgcga catgcgcgcg aacctcaaga accagcgccc cgcgctgctc
    12361 tggttcaccg gcctgtcggg gtcgggcaag tcgacgatcg cgaaccttgt cgagaaaaag
    12421 ctgcaccgga tgaaccggca cagcttcctg ctcgacggcg ataatgtccg ccacggcctc
    12481 aaccgcgacc tcggctttac cgaggctgac cggatcgaga atatccgccg cgtcggcgag
    12541 gtcgcgaagc tgatgagcga tgcggggctg atcgtgatca ccgccttcat ctcgccgttc
    12601 cgcgccgagc gcgagatggt gcgcggcatg ttgcccgagg gcgagttcat cgaagtgttc
    12661 atcgatacgc cgctcgccga ggccgagcgc cgcgacgtca agggcctcta caaaaaggcg
    12721 cgcgcgggac agctcaagaa cttcaccggg atcgatagcc cctatgaggc gcccgagagc
    12781 ccggaaattc ggatcgacac gaccgcgatg acgcccgaag aggcggccga cctgatcatc
    12841 gaccggctgc tgggttaagg gggacgcgtg ggagagagtg acgaacagct cgccgagcgg
    12901 ttggcgacgg ccgcgggccg catcctgctc gacctgcgtg caaagggcga gctcgatggc
    12961 aaggcgctcg ggaaagcggg cgacgagcag gcgaatgcgc tgctctgccg cgaaatccgc
    13021 gccgcgcgtc ccgacgatgc gcttttgtcc gaagaggaaa aggacaattt cgcccgctgc
    13081 gggcaaagcc gcgtgtggat cgtcgatccg ctcgacggca cgcgcgaata tggcgagggc
    13141 agggacgatt gggcggtgca tgtcgccttg gcgatcgacg gcgtcgccac tgtcggtgcg
    13201 gtcgccttgc cggggctcgg tctcacgctc acctcgggcg cgccggcgcc gcttcagccc
    13261 gccaaccagc cgctcaagat gcttgtcagc cggacgcgcc ctgcggccga agcggtgttc
    13321 gtcgccgaaa agcttggcgc cgaactgctc gcgatggggt cggcgggggc caaggcgatg
    13381 gccgtggtgc gcggcgaggc cgacatctac ctccacaccg gcggccaata tgagtgggac
    13441 aattgcgccc cggtcgcggt cgcgcaggcg gcggggctgc acgtcagccg cgtcgacgga
    13501 tcgccgcttc gctacaataa tccggaaacc tatctgcccg atctgctgat ctgccgaaaa
    13561 gagctcgccg cagaggtgct gcgtctcgcc gcggaatatt cgcccgcgga ttagcgtctg
    13621 acgccgagcg ttgccgccct gtttcggtga aaggcgattt gagcgcaaga atcgggtcgc
    13681 cgcgctctcc gccgcgtcga tagaccgcca tgaccaatgt caggaaaggc acgtcatgga
    13741 actggaagac aagaccatca tcgtcaccgg cgcgagcagc gggatcggtg cggcggcggc
    13801 gaagctgttc gcatcggaag gtgcgaatgt ggtgctgggc gcgcgccgcg gcgcggagct
    13861 cgaagccctt gtagcggcga tcaacaaaac cgatggccgg gcggtgttcc tcgccggcga
    13921 cgtgcgggac gagggatatg ccggggcgct tgtcgatctt gcgggaaagg cgttcggcgg
    13981 actggacggc gcgttcaaca atgccggtat cgtcggcgaa atgcagcctg tccccgacat
    14041 ggccatcggc aactggaccg atgtgatttc ggtcaatctg acggcagcct ttctggccgc
    14101 gaaggcgcag ataccggcga tgaaggcgcg cggtcggggc tcgatcgtct tcacctcgtc
    14161 gttcgtgggt ttcagcaacg gcggtatgcc cggcatgggg gcctatgcgg catccaaggc
    14221 gggactgatc ggctttgccc agtcgctggc gtcggaccat gccgccgagg gcataagggt
    14281 caatgcgctg ttgcccggcg gcacgatcac gccggccggg ggcgaaggga acccggccgc
    14341 tctggagttt atcgccgggt tgcatccgat gaaacgaatg gcggcccccg tggagatcgc
    14401 gcaagcggcg ctgttcctcc tgtccgaccg gtcgagcttc atgacgggaa gcccgatgat
    14461 cgccgatggt ggaatctcgg tccggttggc atgatggcgg caggtccggg atggcaggaa
    14521 gggaacgtgg gttcgactgc aaccggcgct caatgacttc gtcattcccg ccgcattttt
    14581 cgcggcgggc gattgccagc gtgatcgggg cgccctattc cgtttcatcg agaaaccgaa
    14641 cacgggagac gcatgatgac cgacctgcct gaaaccaacc tcaccatgct gacgctcgtg
    14701 aagcccgagg ggcagcttga ggtatcgctc gaacggcgcc caatgcctgt cccgaagcca
    14761 cacgaggtgc tggtgaaggt cctcgccgct ccgatcaacc cgtcggacct cggcctgctg
    14821 ttcggcgggg ccgacatgtc gaccgcgcgg gcatcgacgc gcgatggcct gccggtcctc
    14881 accgccgatg tgccgcccgc ggggaagcgc gcgat
//
LOCUS       DATMSU010000493         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_27023, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000493 DATMSU010000000
VERSION     DATMSU010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27023"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /gene="rpoN"
                     /locus_tag="VN034_13130"
     CDS             <1..1284
                     /gene="rpoN"
                     /locus_tag="VN034_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWV61599.1"
                     /translation="GGGDEPAGDAPAAEAASLEADQALSADGGSENDLDVDFVAESFH
                     HDSASDNVGLSGSGEDIDFDSFAEHEGTLHDHLLAQVGERFGGIEAIVAGQLVALIDE
                     AGYLRADLAELAAQLGVPLALLEEVLAGIQCFDPSGVGARDLAECIAIQAREADRYDP
                     AMATMIAHLDLVAKGAFPQLKRICGVDDEDLADMIRELRGYDPRPGLKFGGEGAPAVV
                     PDLYIRQTAKGWAVEINSGTLPRLLVNRRYYTELAQGAAAKSKAWLSEQLAGANWLVR
                     ALDQRQRTIVKVASEIVKQQEGFFLNGVAHMRPLTLRQVAEAIGMHESTVSRVTSNKY
                     LSCARGLFELKYFFSSGIAATAGDGAVSAEAVKSRIKAMIESEDAKAILSDETIAQKL
                     SAEGHDIARRTVVKYREAMGYGSSVQRRRQKALAG"
     gene            1375..1500
                     /gene="ykgO"
                     /locus_tag="VN034_13135"
     CDS             1375..1500
                     /gene="ykgO"
                     /locus_tag="VN034_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HWV61600.1"
                     /translation="MKIRNSLKSLKDRHRDNRVIRRRGRTYVINKTNRRFKARQG"
     gene            1598..2206
                     /locus_tag="VN034_13140"
     CDS             1598..2206
                     /locus_tag="VN034_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWV61601.1"
                     /translation="MIRQSVIFDVGRVLFDWDLRFLFAKLIDDKDRLEWFVTNVVTPE
                     WHFQHDAGRPLAEMVPELKAEFPDHAPLIDAYAARFNETIPGPVPGSIELVERLDDAG
                     VQLFAITNFGHEFWEGFRPTQPVFDRFRDIIVSGTEKLMKPDPAIYALAIERFGIDPA
                     GAIFIDDNAANVAGAESAGIAAHHFRGAMELERDLVARGYLT"
     gene            complement(2194..3177)
                     /locus_tag="VN034_13145"
     CDS             complement(2194..3177)
                     /locus_tag="VN034_13145"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWV61602.1"
                     /translation="MIRALLLLALACLALPAAAQPISAADRGQTIDEIARLLATRYVD
                     ADKGQRIAAELRRAKAQWRDIADGPAFAKALTGWLRQVSGDGHFAVDYSASPIPADGG
                     DKAFLDAEIERYYGAHVNHGVQKIERLDGNIMLIDLRVFPPPTLGGDVIAAMMTVAAQ
                     GDALIIDLRKNGGGMETVDTIVSQLVPAGSPLSGSFDRPSGKTTPHVSPAPPEGRRFG
                     DTKPVYILTSKRTFSAAEALAYDLQALKRATIVGEVTGGGANPFEYRRVHPHFALSLP
                     EKRSVNPITGTNWQDVGVKPDVAVPADQALETALSLAREALAKAPGGGQVK"
     gene            complement(3174..>3385)
                     /locus_tag="VN034_13150"
     CDS             complement(3174..>3385)
                     /locus_tag="VN034_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61603.1"
                     /translation="RDRRERRFALTIAARAMIPDLEATWAATAGAAQALDAALSQPLG
                     AAVDAALEQLERQSFGDLIKKERAR"
BASE COUNT          578 a         1094 c         1139 g          574 t
ORIGIN      
        1 ggcggcggtg acgaacccgc cggcgatgcg cccgcggccg aggccgcgtc gctcgaagcc
       61 gatcaggcgc tctcggccga cggcgggagc gaaaacgacc tcgacgtcga tttcgtggcc
      121 gaaagcttcc accacgacag cgcgagcgac aatgtcggcc tatcggggag cggcgaggat
      181 atcgatttcg acagcttcgc cgagcatgag ggcacgctcc acgaccatct gctcgcgcag
      241 gtcggcgagc ggttcggcgg catcgaggcg attgtcgcgg gacagctcgt cgcgctgatc
      301 gacgaggcgg ggtatctgcg cgccgatctg gccgaactcg ccgcgcagct cggcgtgccg
      361 ctcgcgctgc tcgaagaggt cttggcgggc atccaatgct tcgacccgtc gggcgtcggc
      421 gcgcgcgacc tcgccgaatg catcgcgatc caggcgcgcg aggccgaccg ctacgacccg
      481 gcgatggcga cgatgatcgc gcatctcgac ctcgtcgcca agggcgcctt tccgcagctc
      541 aaacgcatct gcggggtcga tgacgaggat ctcgccgaca tgattcgcga gctgcgcggt
      601 tacgatccgc gacccgggct taaattcggc ggcgagggcg cacccgcggt ggttcccgac
      661 ctttatatcc gccagaccgc gaagggctgg gcggtcgaga tcaacagcgg caccctgccc
      721 cgcctgctcg tcaaccgccg ctattatacc gaactcgcgc agggcgccgc cgccaagagc
      781 aaggcgtggc tttccgaaca gctcgcgggc gccaactggc tcgtccgcgc gctcgaccag
      841 cgtcagcgca cgatcgtcaa ggtcgcgagc gagatcgtga agcagcagga gggtttcttc
      901 ctgaacggcg tcgcgcatat gcgtccgctg acgctgcgcc aggtcgccga ggcgatcggc
      961 atgcacgaat cgaccgtcag ccgcgtgacg agcaacaaat atctgagctg cgcgcgcggc
     1021 ctcttcgaac tcaaatattt cttctcgagc ggaatcgccg cgaccgcggg cgacggcgcg
     1081 gtgtcggcgg aggcggtgaa gagccggatc aaggcgatga tcgagagcga agatgcgaaa
     1141 gcgatcctct ccgacgagac gatcgcgcaa aaactgtcgg ccgagggcca cgacatcgcg
     1201 cggcgcacgg tggtcaaata tcgcgaggcg atgggctatg gctcgtcggt gcaaagacgg
     1261 cggcagaaag cactggcggg ctgattttag ccggaaaggc ggttgacttt ccggcccctt
     1321 ccccataggt gcgccgcttc gcgtgcaacg ctcgaaattt tacggaacag aacgatgaag
     1381 attcgcaaca gcctgaagtc gctgaaggac cgccaccggg ataaccgcgt gatccgtcgc
     1441 cgtggccgca cctatgtgat caacaagacc aaccgccgct tcaaggcgcg ccagggctga
     1501 tgtccctgcc ggggcgtttc ccgccccgcg cgcatttgcg agccagcgtg acgccgtcgg
     1561 atgcttccgg cggcgctttc gcgtgtccgg agccagcatg attcgccaga gcgtgatctt
     1621 cgacgtcggc cgcgtcctct tcgattggga cctgcgcttt ctgttcgcga agctgatcga
     1681 cgacaaggac cggctcgagt ggttcgtcac caacgtcgtc acgcccgagt ggcatttcca
     1741 gcacgacgcc gggcgtccgc ttgccgagat ggtccccgaa ctcaaggccg aattccccga
     1801 tcacgcgccg ctgatcgacg cctatgcggc gcgtttcaac gaaacgatcc cggggccagt
     1861 gcccggcagt atcgaactgg tcgagcggct cgatgacgcg ggcgtgcagc tcttcgcgat
     1921 caccaatttc gggcatgaat tctgggaagg tttccgcccg acgcagccgg tgttcgaccg
     1981 tttccgcgac atcatcgtgt cgggcaccga gaaactgatg aagcccgatc ccgcgatcta
     2041 tgccctcgcg atcgagcgat tcggcatcga ccccgcgggc gcgatcttca tcgacgacaa
     2101 tgcggccaat gtcgcgggcg ccgaatcggc cgggatcgcg gcgcatcatt ttcgcggcgc
     2161 gatggaatta gagcgcgacc tcgtcgcgcg cggctatttg acctgaccgc cgccgggcgc
     2221 tttggcgagt gcttcccttg cgagcgatag cgcggtttcg agcgcctggt cggcggggac
     2281 cgcgacgtcg ggcttgaccc cgacatcctg ccagttggtt ccggtgatcg ggttgaccga
     2341 ccgtttctcg ggcaggctca gggcaaaatg cgggtgcacg cggcgatatt cgaacgggtt
     2401 ggcgccgcct cccgtgactt cgccgacgat cgtcgcgcgt ttcagggctt gaagatcgta
     2461 ggcgagcgct tcggcggccg agaaggtgcg cttgctggtc aggatataaa ccggcttcgt
     2521 gtcgccgaac cggcgccctt cgggcggcgc aggtgagaca tggggcgtcg tcttgccgct
     2581 cggacggtcg aaactgccgc tgagcggcga gcccgcggga acgagctggc tgacgatcgt
     2641 gtcgaccgtt tccatgccgc cgccattctt gcgcaggtcg atgatcagcg catcgccctg
     2701 cgccgcgacg gtcatcatcg cggcaatgac atcgccgcca agcgtcgggg gcgggaagac
     2761 gcgaaggtcg atcagcatga tattgccgtc gagacgttcg attttctgca cgccatgatt
     2821 gacgtgcgcg ccataatagc gctcgatctc cgcgtcgaga aaggccttgt cgccgccgtc
     2881 ggcggggatc gggctggcgc tgtaatcgac cgcgaaatgc ccatcgcccg aaacctgccg
     2941 cagccagccg gtcaacgctt tggcgaacgc ggggccgtcg gcgatgtccc tccattgcgc
     3001 cttcgcccgg cgcaactcgg cggctatccg ctgccccttg tcggcgtcga catagcgcgt
     3061 cgcgagcaag cgagcgattt cgtcaatcgt ttgcccccga tcggcggcgc tgatcggctg
     3121 cgccgcggcg ggtagcgcga ggcaagcgag cgccagcaaa agcagggcac gtatcatcgg
     3181 gcgcgttcct tcttgatgag atcgccaaac gactggcgtt cgagttgttc gagggcggca
     3241 tcgacggccg cgcccaaggg ttgcgacagc gccgcatcga gcgcttgggc ggcaccggcg
     3301 gtcgcggccc aggtcgcttc gagatcgggg atcatggcgc gcgccgcgat ggtgagggca
     3361 aaacgccgtt cgcgccggtc gcgac
//
LOCUS       DATMSU010000494         5574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_168910,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000494 DATMSU010000000
VERSION     DATMSU010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168910"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VN034_13155"
     CDS             complement(<1..198)
                     /locus_tag="VN034_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61604.1"
                     /translation="MFRALSTTTIRLFVPLLAGASLRDRAIGSVGALIGIAVVGVFCV
                     LFFPAATPWIVAPVGASAVLLF"
     gene            560..2626
                     /locus_tag="VN034_13160"
     CDS             560..2626
                     /locus_tag="VN034_13160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWV61605.1"
                     /translation="MRNHLLTSAIALTAALSTPALARPMTEVDLATLKRVAAPAASPD
                     GRWVVFQMTETEADSYKRSTGLWLVDRQAKGAQPTPVADTAGKNETSPAFDKDGFLYF
                     LSNASGKDQVWRIDPKAGGAATQVTDTRADVAGFKISPDATRLLAWGDIAKECTDFGC
                     EAKDKGALPGPGTGRLYKDGAGFVRHWDHWETPGTYSRPFVFNLAGGKATAARPADAG
                     LIGDTPSKPFGGGEELAWGADSRTVFFTLRKADRNEPMSTNLDIYSWLVDSRMAPTNL
                     TQDNQATDTLPTPSPDGKWLAYVAMARPGYEADRLVLMLRNLESGETKKLTDAWDRSV
                     GSIAWAPDGKSLYVTAQDTLEHPVFRVDAATGKVEKLKASPDAFAGNIGDVTPLPGGG
                     LLYSRNSALAPTDLFVRDAKGKVGQLTAVNADRLAEFDPVELERIQFAGANGDKVWGM
                     ILKPVSPVQKLPVAFIVHGGPQGSFGNSWSTRWNPRLFAQQGYGVVTVDFHGSTGYGQ
                     AFTDSINKDWGGKPLEDLKLGLAAAGKQDAQLDITNACALGASYGGYMMNWIAGQWSD
                     GFKCLVQHDGVFDLRAMAFETEELWFDEWDHGGPWWERTDPEKWNPVNHVDKWKTPML
                     VITGEKDFRIPYTQGLAAFTALQRRGVPSQLLVFPDENHWVLKGANSVQWHQTVLGWL
                     GEHLKK"
     gene            2800..>5574
                     /locus_tag="VN034_13165"
     CDS             2800..>5574
                     /locus_tag="VN034_13165"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWV61606.1"
                     /translation="MPMEKTFDPAAIEAKWAHEWESRGLFRPARPDATPFTIVNPPPN
                     VTGALHIGHALDNTLQDVLVRYERLRGKDALWVVGMDHAGIATQMVVERQLEQQQDKR
                     TNYSREDFIDKVWQWKAESGGQITGQLRRLGCSMDWSREQFTMDPHFTRAVVKVFVDL
                     HKKGLIYRDKRLVNWDPKLKTAISDLEVETHEIQGGFWHFKYPLADGVTLDDGRDHIV
                     VATTRPETMLADMAVAVHPDDPRYKSVIGKDILQPITGRRFKIVPDEHADPELGSGAV
                     KITPGHDFNDFEVGKRAGFKPAEMLNMLDGDANVIQTADGLIPDEYLGLHRFKRDGKD
                     GARELVVTRMKEAGFLIPHTDKDGGEHDAEPRTIQTPFGDRGGVVIEPWLTDQWYVDA
                     ETLAQPPMAAVRDGRINIVPKTWEKTFFNWMENIQPWCVSRQLWWGHRIPAWRSAGEA
                     VTDVLGQTYIAETGNMYVEETETGAIQAAEKDADGRQVTVFDSFAEVATKASQLGSAE
                     FNETIYAIVRDPDVLDTWFSSALWPFATLGWPEDTELLKRHYPNDVLISGFDILFFWD
                     ARMAMQGMEFMRDVPWKTLYLHGLVRAPDGAKMSKSKGNVVDPLGLIDQYGADALRFF
                     MSAMESQGRDIKMDDARLAGYRNFATKLWNAARFCEANGIAASTSFEAPPATLPVNRW
                     IIGEVAATVAAMETAFAAYRFDEAANAIYSFAWDRFCDWYLELIKGAIDDETKAVAGW
                     VLDQILVMLHPFMPFITEELWTGLGDRADYPLITAKWPAPNAARDTDASADIDWLIKL
                     VSELRTAKAELGLPPGARLTAHFPASLKDRTDKLAAQLDRLARLESVSFDPAPAGASA
                     QLVVEGETITVPLEGVIDIAAERDRLTKALAAAAKERDGLAGRLNNPSFVERAKPEAV
                     EKARADHAAKD"
BASE COUNT         1081 a         1907 c         1730 g          856 t
ORIGIN      
        1 gaacagcagc accgccgacg cgccgaccgg cgcgacgatc cacggcgtcg ccgcgggaaa
       61 gaacagcacg caaaacacgc cgacgaccgc gattccgatc agcgcgccga cgctgccgat
      121 cgcgcggtcg cgcaggctcg cacccgcaag cagcggcacg aacaggcgga tggtggtggt
      181 cgacagcgcg cggaacaagc gattctcctc tttcgggccg cgccgatgga ccagaaaggc
      241 gctgcgatcc agacagcttt gattggaccg cgcgaagctc gattgagagc aatgctggaa
      301 aaccgatcat cttggatcga aacaccctcc ctcttcgatc acgaacgact gcaagcggct
      361 tgtagctgtc gatcgagacg tgcgccccgg cgaaagccgg ggtccagaac gctgacgcga
      421 actcagcgca tttccgctct gggccccggc tttcgccggg gtgcacaagg ggcagctcac
      481 caccccaaaa cagacatcac ggccacagtt tggcgacgta acgacttgat cacatacgcc
      541 gcacggctaa accgctttca tgcgaaacca tcttctcacc agcgcgatcg cgctcaccgc
      601 cgccctttcc actcccgccc tcgcccgtcc gatgaccgag gtcgacctcg cgacgctgaa
      661 acgtgtcgcc gcgccggcgg catcgcccga cggccgctgg gtcgtgttcc agatgaccga
      721 gaccgaggcc gatagctaca aacgctcgac cggcctctgg ctcgtcgacc gtcaagcgaa
      781 gggtgcacag ccgacgccgg tcgccgacac cgcgggaaag aacgagacct cgcccgcgtt
      841 cgacaaggac ggtttcctct atttcctctc gaacgcgtcg ggcaaggatc aggtctggcg
      901 tatcgatccg aaggcgggcg gcgccgcgac gcaggtgacc gacacccggg ccgacgtcgc
      961 gggcttcaaa atctcgcccg acgcgaccag gctgctcgcc tggggcgaca tcgcgaagga
     1021 gtgcaccgat ttcggctgcg aggcgaagga taagggcgcg ctccccggtc ccggcaccgg
     1081 ccgtctctac aaggatggcg cgggcttcgt ccgccattgg gaccattggg aaacccccgg
     1141 cacctacagc cgccccttcg tcttcaacct cgcaggcggc aaggcgaccg ccgcacgccc
     1201 cgccgacgcc ggcctgatcg gcgacacccc ctccaagccc ttcggcggcg gcgaggaact
     1261 cgcgtggggc gccgacagcc gcacggtctt tttcacgctg cggaaggccg accgcaacga
     1321 accgatgtcg accaacctcg atatctacag ctggctcgtc gacagccgga tggcgcccac
     1381 caacctgacg caggacaatc aggcgaccga cacgctgccg acgccgtcgc ccgacggcaa
     1441 atggctcgct tacgtcgcga tggcgcgccc gggttacgag gccgaccggc tcgtgctgat
     1501 gctccgcaac ctcgaaagcg gcgagacgaa gaagctcacc gacgcgtggg accgctcggt
     1561 cggttcgatc gcctgggccc ccgacggcaa gtcgctctat gtcaccgcgc aggacacgct
     1621 cgaacatccg gtgttccgcg tcgacgccgc gacgggcaag gtcgagaaat tgaaggcctc
     1681 gcccgatgcc ttcgcaggca atatcggcga cgtcaccccg ctccccggcg gcggtctgct
     1741 gtattcgcgc aacagcgcgc tcgcgccgac cgatcttttc gttcgcgatg cgaagggcaa
     1801 ggtcggccag ctcaccgcgg tcaacgccga tcgcctcgcc gaattcgacc ccgtcgagct
     1861 tgagcgcatc cagttcgcgg gcgcgaacgg cgacaaggtg tggggcatga tcctgaaacc
     1921 cgtcagcccg gtgcagaagc tgccggtcgc cttcatcgtc catggcggac cgcagggcag
     1981 cttcggcaac agctggtcga cgcgctggaa cccgcgcctc ttcgcgcagc agggctatgg
     2041 cgtcgtcacc gtcgatttcc acggctcgac gggttacggc caggccttca ccgacagcat
     2101 caacaaggat tggggcggca agccgctcga ggacctcaag ctcggcctcg ccgccgcggg
     2161 caagcaggat gcgcagctcg acatcaccaa cgcctgcgcg ctcggcgcct cctacggcgg
     2221 ctatatgatg aactggatcg cgggccagtg gagcgacggc ttcaaatgcc tcgtccagca
     2281 tgacggcgtc ttcgatctcc gcgccatggc gttcgagacc gaggaattgt ggttcgacga
     2341 atgggatcat ggcgggccgt ggtgggaacg caccgacccc gaaaagtgga acccggtcaa
     2401 tcatgtcgac aagtggaaga ccccgatgct ggtgatcacc ggcgaaaagg atttccgcat
     2461 cccctacacc cagggcctcg ccgccttcac cgcgctccag cgccgcggcg tcccgtcgca
     2521 attgctcgtc ttccccgacg agaaccactg ggtgctcaag ggcgcgaaca gcgtccagtg
     2581 gcaccagacg gtgttgggtt ggctgggtga gcatttgaag aaataggaac ctccaaaccc
     2641 gttcgtgtcg agcgaagtcg agacaccccg aaggcgcgcg ccaacgatgg gcatctcgac
     2701 ttcgctcgat gcgaacggga ctcttgtgtg ccgggcatga tgggagcggc ttcgttcccc
     2761 cttgccgcct cgccccatgg ctggcaaagg cgcccggcta tgccgatgga aaaaaccttc
     2821 gatcccgccg ctatcgaggc gaaatgggcc catgagtggg aaagccgcgg gctgtttcgt
     2881 cccgcgcgcc ccgacgcgac ccccttcacg atcgtcaacc cgccgccgaa cgtcaccggc
     2941 gcgctccaca tcggccatgc gctcgacaac acgctgcagg acgtgctcgt ccgctacgaa
     3001 cgcctgcgcg gcaaggatgc gctgtgggtc gtcggcatgg atcatgccgg catcgcgacg
     3061 cagatggtgg tcgagcgcca acttgagcag cagcaggaca agcgcaccaa ttacagccgc
     3121 gaagatttca tcgacaaggt gtggcagtgg aaggcggaaa gcggcggcca gattaccggc
     3181 cagctccgcc gcctcggctg ctcgatggac tggagccgcg agcaattcac gatggacccg
     3241 catttcacgc gcgcggtggt caaggtgttc gtcgacctcc acaaaaaggg gctgatctac
     3301 cgcgacaagc ggctcgtgaa ctgggatccg aagctcaaga ccgcaatttc ggacctcgag
     3361 gtcgagacgc acgagattca gggcggcttc tggcacttca aatatccgct cgccgacggc
     3421 gtgaccctcg acgacggacg cgatcatatc gtcgtcgcga cgacgcgccc cgaaacgatg
     3481 ctcgccgata tggcggtcgc ggtgcacccc gacgatccgc gctataaaag cgtgatcggc
     3541 aaggatattc tgcaaccgat caccggccgc cgcttcaaga tcgttcccga cgaacatgcc
     3601 gaccccgagc tgggttcggg cgcggtgaag atcaccccgg ggcatgattt caacgacttc
     3661 gaggttggca agcgcgcggg gttcaagccc gccgagatgc tcaacatgct cgacggcgac
     3721 gcgaacgtca tccagaccgc cgacgggctg attccggacg aatatctcgg cctccaccgc
     3781 ttcaagcgcg atggcaagga tggcgcgcgc gaactcgtcg tgacgcgcat gaaggaagcg
     3841 ggcttcctca tcccgcacac cgacaaggac ggtggcgaac atgacgccga gccgcgcacg
     3901 atccagacgc cgttcggcga ccgcggcggc gtggtgatcg agccctggct caccgatcaa
     3961 tggtatgtcg acgccgaaac gctggcgcag ccgccgatgg cggcggtgcg cgacgggcgc
     4021 atcaacatcg tcccgaagac ttgggaaaag accttcttca actggatgga gaatattcag
     4081 ccgtggtgcg tctcgcggca gctctggtgg gggcaccgga ttcctgcgtg gcggtcagca
     4141 ggcgaggctg taaccgacgt actggggcaa acatacattg ccgaaaccgg aaatatgtac
     4201 gtcgaagaaa cagagaccgg tgcgatacag gccgctgaaa aggatgctga cggacggcaa
     4261 gtcaccgttt ttgacagctt tgcagaagtg gccaccaaag catctcagtt gggctcggca
     4321 gagtttaatg agacaatata cgcaatagtc cgcgaccccg acgtcctcga cacttggttc
     4381 tcctccgccc tctggccgtt cgctaccctc ggctggcccg aggacaccga actcctcaag
     4441 cgccactacc ccaacgacgt cctcatctcg ggcttcgaca tcctcttctt ctgggacgcc
     4501 cgcatggcga tgcaggggat ggagttcatg cgcgacgtgc cgtggaagac gctctacctc
     4561 cacggcctcg tccgcgcgcc cgatggcgcg aaaatgtcga agtcgaaggg caatgtcgtc
     4621 gacccgctcg ggctgatcga ccaatatggc gccgacgcgc tgcgcttctt catgtcggcg
     4681 atggaaagcc agggccgcga catcaagatg gatgacgcgc gcctcgcggg ttatcgcaat
     4741 tttgcgacca agctgtggaa cgccgcgcgc ttctgcgaag ccaatggcat tgcggcctcg
     4801 accagcttcg aggcgccgcc cgcgacgctg ccggtcaacc gctggatcat cggcgaggtc
     4861 gccgcgaccg tcgccgcgat ggaaaccgcc ttcgccgcgt accgcttcga cgaggccgcc
     4921 aacgcgatct acagcttcgc gtgggaccgc ttctgcgact ggtatctcga actgatcaag
     4981 ggcgcgatcg atgacgagac gaaggccgtc gccggctggg tgctcgacca gatcctcgtc
     5041 atgctacacc ccttcatgcc cttcatcacc gaagagctgt ggacggggct cggcgaccgc
     5101 gccgactatc cgctgattac cgccaaatgg cccgcgccga acgccgcgcg cgataccgac
     5161 gcgagcgccg acatcgactg gctcatcaag ctcgtcagcg agctgcgcac cgccaaggcc
     5221 gaactcggcc tgccgccggg cgcacgcctg accgcgcatt tcccggcctc gctcaaggac
     5281 cgcacggaca agctcgcggc gcagctcgac cgcctcgcgc ggctcgagag cgtcagcttc
     5341 gatcccgccc ccgcgggtgc ctcggcgcag ctcgtcgtcg agggcgagac gatcaccgtt
     5401 cccttggagg gcgtcatcga catcgccgcc gaacgcgacc gcctgaccaa ggcgctcgcc
     5461 gccgcggcga aggaacgcga cggcctcgcc ggccgcctga acaacccgtc gttcgtcgaa
     5521 cgcgccaagc ccgaagccgt cgaaaaagcg cgcgccgacc acgccgccaa ggac
//
LOCUS       DATMSU010000495         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_140829,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000495 DATMSU010000000
VERSION     DATMSU010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140829"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="VN034_13170"
     CDS             complement(<1..1747)
                     /locus_tag="VN034_13170"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HWV61607.1"
                     /translation="MANLTAQPDKRWGRIVALSAAAMLLTGASAPELSGLVARYEHFA
                     EDYPMEAGRSDPSRSREGWPDESPEGMARRKAELERIAAELHALDGATLTGEDRVNRD
                     YLRELIGWRIEGIDFDERRFAFVAHEGFYNTPYSLARGLDIKSEAEARAWAERMRGIP
                     RYFAEQRANLERGVATGWTHPEVVIDVAVAVLRNQIAVPVADDPMLASLSAYPAVRAE
                     AQRLIEREIRPAQKAALAYIDGPYRAKARKDLGIAAVPGGRAYYDFLLRYYTTTDLTA
                     EQVHAMGLAEVQRICGEMDKVIASTGFEGSFADWLRFLRTDPRFYATSREGLIEKYAA
                     ASKRIDGVLPRYFSVLPRQPFTIVPVPRELEEGYTTARGGGGGDFAKGFAGTFVVNTS
                     HLDQRPLYEVPALSLHEAAPGHHTHGALNNENPELPSFRRARDLLAFREGWALYAERL
                     GHEMGVYRDAYEKFGSLSTEMWRACRMVVDTGIHVMGWSYAQARQCFAQNTALADINI
                     DTELARYIGAPGGAVAYKVGELKIVEMRRRAEAALGERFDIRAFHDLILAQGQLPMTM
                     LEAKVDAWIAARKRSG"
     gene            1849..2319
                     /locus_tag="VN034_13175"
     CDS             1849..2319
                     /locus_tag="VN034_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWV61608.1"
                     /translation="MDVFLSGNPGAAAFRVKRYPFYLLNRLVSRYNSIIDARLRAIGL
                     DIPNWRVLMILGEKSPRGAREIAEAAVINLSTMTRIIQRMAAAELVTAAPSRDDARVT
                     LVALAPAGKAQLKKARQVTAPIYEHLVTGLAPEEFEQLIALLGRLHDNLEPLAP"
     gene            complement(2333..>2929)
                     /locus_tag="VN034_13180"
     CDS             complement(2333..>2929)
                     /locus_tag="VN034_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61609.1"
                     /translation="GTVRAGRVVLAANGGNAALHPAMRRTTLPLHVFQFATAPLDAAQ
                     REVILPQGGAFTDKVPYLFSARLDGHGHLISAFPVSFLVRGDEACYREARRRLKQHFA
                     AMPDPQVDYLWRGLAWVNTSFLPEVYDLGDGAIAIQACNGRGISTNSVIGAEVAEMLA
                     TGNRDALSVQPREPSPVRFHAGVALLPKLLMSMAYLSN"
BASE COUNT          508 a          985 c          948 g          488 t
ORIGIN      
        1 ctcctgaacg tttgcgcgcc gcgatccacg cgtcgacctt ggcctcgagc atcgtcatcg
       61 gcagttgccc ctgcgcgagg atcaggtcgt ggaaggcgcg gatgtcgaac cgctcgccca
      121 gcgccgcctc ggcgcgccgg cgcatctcga cgatcttgag ctcgccgacc ttgtacgcga
      181 ccgcgccgcc cggcgcgccg atatagcgcg ccagttcggt gtcgatgttg atgtcggcga
      241 gcgcggtatt ttgggcgaag cattgccggg cctgcgcata gctccaaccc atgacgtgaa
      301 tcccggtgtc gacgaccatc cggcacgcgc gccacatctc ggtcgaaaga ctgccgaatt
      361 tttcataggc atcgcgatag acgcccattt cgtgccccag ccgttcggca tagagcgccc
      421 agccttcgcg gaaggcgagc aggtcgcgcg cgcggcggaa cgaaggcagt tcaggattct
      481 cgttgttgag cgcgccatgc gtgtggtgcc cgggcgccgc ctcgtgcagg ctgagcgccg
      541 gaacctcgta caacggccgc tgatcgagat gcgaggtgtt gacgacaaag gtgccggcaa
      601 agcccttggc gaaatcgccg cccccgccgc cgcgcgcggt cgtatagcct tcctccagct
      661 cgcgcggcac cgggacgatg gtgaagggct gacgcggcag caccgagaaa tagcgcggca
      721 gcacgccgtc gatccgtttc gacgcggcgg cgtatttttc gatcaaacct tcgcggctgg
      781 tggcatagaa gcgcgggtcg gtccgcagga agcgcagcca gtcggcaaag ctcccctcga
      841 aaccggtcga agcgatgacc ttgtccatct cgccgcaaat ccgctgcact tccgcaagac
      901 ccatcgcatg gacctgttcg gcggtcaggt cggtcgtcgt ataatagcgc agcaggaaat
      961 cataataggc gcgcccgccg ggcacggccg cgatgccgag atccttgcgc gcttttgccc
     1021 ggtagggacc gtcgatatag gcaagcgccg ccttttgcgc cggacggatc tcgcgctcga
     1081 tcaaccgctg cgcctctgcg cgtaccgctg gatatgcact cagcgacgcg agcatcgggt
     1141 cgtcggcgac aggaaccgcg atctggttgc gaagcaccgc aaccgcgaca tcgatcacga
     1201 cctcgggatg cgtccagccc gtagcgacgc cgcgttcgag attggcgcgc tgttcggcga
     1261 aatagcgcgg aatgccgcgc atccgctccg cccacgcgcg tgcttcggct tcgcttttga
     1321 tgtcgagccc gcgtgccagc gaatagggcg tgttgtagaa gccttcatgc gcgacgaaag
     1381 cgaagcggcg ctcgtcgaaa tcgatgcctt cgatccgcca gccgatcagt tcgcgcagat
     1441 agtcgcgatt gacgcggtcc tcgcccgtca gcgtcgcgcc atccagcgca tggagctctg
     1501 cggcaatccg ttcgagttca gccttgcggc gcgccatgcc ctcgggcgat tcatcgggcc
     1561 agccttcgcg tgaacgcgac gggtcggacc gccccgcttc catcggataa tcctcggcga
     1621 aatgctcgta tcgcgcgacc agcccggaca gctcgggcgc gctcgcgccg gtcagcagca
     1681 tcgccgcggc gctcagtgcc acgattcgtc cccagcgctt gtccggctgc gcagtcagat
     1741 tggccatcgg gatttccttt cgcgggcggg tgccgcatcc ttcgcggatc gatcatgcat
     1801 cggacggggc ggattgaaac gggcaggcat tcggctaaag cggcagctat ggacgtcttc
     1861 ctgtcgggca atcccggcgc cgccgccttt cgggtcaaac gatacccctt ctacctgctc
     1921 aacaggctgg tgagccggta caacagcatc atcgacgcgc ggctgcgcgc gatcggcctc
     1981 gatatcccga actggcgggt gctgatgatc ctgggcgaaa aatcgccgcg cggcgcgcgc
     2041 gaaatcgccg aggccgcggt gatcaacctg tcgacgatga cgcgcatcat ccagcggatg
     2101 gccgccgccg aactggttac ggcggcgccc agccgcgacg acgcgcgcgt cacgctggtc
     2161 gcgcttgcgc ctgccggcaa ggcccagctc aagaaggccc ggcaagtcac cgcgcccatc
     2221 tatgagcatc tggtgacggg actcgcgccc gaagagttcg aacagctgat cgcgctgctc
     2281 ggacgccttc acgacaatct cgaaccgctg gcgccttgaa ttccccatcg cctcagttcg
     2341 aaagataggc catcgacatc agcaatttgg gcagcaaggc cacaccggcg tggaatcgca
     2401 cgggtgacgg ctcgcgcggc tgcaccgaaa gcgcatcgcg gttccccgtc gccagcatct
     2461 cggcgacctc ggcgccgata acgctgttcg tggagatgcc gcgcccgttg cacgcctgaa
     2521 ttgcgatcgc gccgtcgccg aggtcgtaaa cttcgggaag gaacgaggtg ttcacccacg
     2581 caaggccgcg ccacagatag tcgacctgag gatcgggcat cgccgcgaaa tgctgtttga
     2641 gccgccgccg cgcctcgcgg tagcacgcct cgtcgccgcg cacgaggaag gacacgggaa
     2701 aggcggagat cagatgcccg tggccgtcga gccgcgcgct gaacagatag ggcaccttgt
     2761 cagtgaaggc gccgccttgc ggtaggatca cctccctctg cgccgcgtcg agcggcgccg
     2821 tcgcgaattg gaagacatgc agcggcaaag tcgtacggcg catcgccgga tggagcgcgg
     2881 cgttgccccc gttggccgcg agcacgacgc ggcccgcgcg cacggtgcc
//
LOCUS       DATMSU010000496         4204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_8890, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000496 DATMSU010000000
VERSION     DATMSU010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4204
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8890"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..2598)
                     /locus_tag="VN034_13185"
     CDS             complement(163..2598)
                     /locus_tag="VN034_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61610.1"
                     /translation="MNARASLLAGLSCLALASTPAAAQDAPSDSYDGNEIIVTATKRD
                     ASLQDVPFSINAQTEQAIERANASTIEDLSRNVAGLTVQNLGPGQSQVSVRGVSAGQI
                     ARDQPGVKEQVGVYLDESVISLSLFTPDLDLFDLNRVETLRGPQGTLFGSGSVGGTLR
                     YITNQPTTDRVEGKVEGNVNLVDGDDFGGHLKGAINLPLSENLAMRAVGYYTRYAGFI
                     NALREGGGESEDVNSGERYGGRFSLRWEPAEGLSITPRVVYQKVTTDGFNRQEVYNLY
                     ANQFTTTRPQVTFEERQQYLLLDEAFEDEVKLFDLTMNYTGDVVGVTSVTTYTDRDIL
                     VSRDASALTGSVSSDLGFPDAGILLPSNLVDTTGVKQFTQEVRVNSAGDGPFQWLIGG
                     YYANVKRDYTQRLPTPGYDAFTNQFFIDACNADPDDCGPGGVPLTAADIANGFGPDSP
                     YNADIPYDLSQIAIFGEVSYDFTERLTATVGGRYYAFDESRRFVSGGLFPNGDNARDK
                     TSSTGFSPRLLLSYDVADGVTLNAQASKGFRLGGVNDPLNLPLCDGGNPGGPDAETFG
                     GRPRYDDETLWNYEGGVKAQFGGITFNAAGFYTKINNLQVTADAGSCSSRIVFNADAH
                     TMGLEFELSASPFTGFDLGLSGSYVEAEFDSTLTRPDGTVIEGIRDGNRLPSVPKFQM
                     AANATYGFPIDAAGDTNAFVTASFQHVGSRYTQPGDQENNPRTFEHGFTFGGAPIGSA
                     TTLDLKLPDYQLVNIGAGIEFPDELEISVYVNNLFDENALLAFDRERGGRARLGFATN
                     QPRTFGVTVRKGF"
     gene            complement(2900..>4204)
                     /locus_tag="VN034_13190"
     CDS             complement(2900..>4204)
                     /locus_tag="VN034_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWV61611.1"
                     /translation="ALPAWAQEAAPAAAEAAAPTPDKGDTAWMMVATVLVMAMIVPGL
                     ALFYGGLVRTKNMASVLTQVLAVAALAMVLWVIYGYGLAFGGDANWLISSGKFFLAGV
                     TADSNVATFTDGVVIPEFVFIAFQMTFSAITVALVLGGLVERMKFSAVMVFAIVWLTI
                     VYYPIAHMVWYLGGDEASTGLIFGWGALDFAGGTVVHINAGIAALVGGFIIGKRRGYQ
                     KDIMAPHSLTMTLIGTGLLWVGWFGFNAGSALEANGSAGLALINTFTATAAGVLFWML
                     TERVLGHKGSLLGACSGAIAGLVAVTPAAGNSGPFGAILLGAIAGIVCCWFVMKIKPK
                     LGFDDSLDVFGIHGIGGLIGSVLTAVTMLPALGGPAGDDYVLGSQLWIQIKSVGVAVL
                     WSAVGSAIAFTIAKAVTGGRVSEEVEREGLDLGEHGERAYNY"
BASE COUNT          784 a         1289 c         1417 g          714 t
ORIGIN      
        1 gcctttcagg ctctcccctc ctgaaactgt tgacccaatg aatgccatat taatgtcatg
       61 ttggaaaggc aaaaaataag accatgctgc acaaatttcg tgcagcattt cagggcgaaa
      121 aaggggcggg gataccgtgg ctccccgccc ctctgctccc gatcagaacc cctttcgcac
      181 cgtgacgccg aaggtccgcg gctggttggt cgcaaagccg aggcgcgcgc gcccgccgcg
      241 ttcgcggtcg aaggcgagca gcgcattttc gtcgaacaga ttgttcacat agaccgagat
      301 ttcgagttcg tcggggaact cgattcccgc gccgatattg accagctgat agtcgggcag
      361 cttgaggtcg agcgtcgtcg ccgacccgat cggcgcgccg ccgaaagtga agccatgttc
      421 gaaggtgcgc gggttgttct cctgatcgcc cggctgcgta tagcggctgc cgacatgctg
      481 gaaggaggcg gtgacgaagg cgttggtgtc gcccgccgca tcgatcggaa agccgtaggt
      541 cgcgttcgcc gccatctgga atttgggcac cgaaggcagg cggttgccgt cgcggatgcc
      601 ctcgatcacc gttccgtcgg ggcgcgtcag cgtcgaatcg aattcggcct cgacatagct
      661 gcccgacagt ccgagatcga agccggtgaa ggggctcgcc gacagctcga attcgagccc
      721 catcgtatgc gcatcggcgt tgaagacgat acgcgacgag cagctgcccg cgtcggcggt
      781 gacctgcaga ttgttgatct tggtatagaa gccggccgcg ttgaaggtga tgccgccgaa
      841 ctgcgccttc accccgcctt catagttcca cagggtttcg tcgtcgtagc gcggccgccc
      901 gccgaaggtc tcggcatcgg ggccgcccgg attgccgccg tcgcagagcg gcaggttcaa
      961 gggatcgttg accccgccga ggcggaagcc cttcgacgcc tgggcattga gcgtgacgcc
     1021 gtcggcaacg tcgtagctca agagcaggcg cggcgaaaag ccggtcgacg aggtcttgtc
     1081 gcgcgcattg tcgccgttgg ggaaaaggcc gcccgagacg aagcggcggc tttcgtcgaa
     1141 ggcataatag cgcccgccga ccgtggcggt caggcgttcg gtgaaatcat agctgacctc
     1201 gccgaagatc gcgatttgcg acaggtcgta agggatgtcg gcattatagg gcgaatcggg
     1261 cccgaagccg ttggcgatgt cggccgcggt aagcggcacg ccgccgggtc cgcaatcatc
     1321 ggggtcggca ttgcacgcgt cgatgaagaa ctggttggtg aaggcgtcgt aacccggcgt
     1381 cggcaggcgc tgcgtatagt cgcgtttgac gttggcataa tagccgccga tcaaccactg
     1441 gaacgggccg tcgcccgccg aattgacacg aacttcctgc gtgaactgct tgaccccggt
     1501 ggtgtcgacc agattcgagg gaagcaaaat gcccgcgtcg ggaaagccga ggtcggacga
     1561 cacgctcccg gtgagcgcgc tcgcatcgcg gctgacgagg atgtcgcggt cggtataggt
     1621 ggtgaccgac gtcacgccga cgacgtcgcc ggtatagttc atcgtgaggt cgaagagctt
     1681 gacctcatcc tcgaacgcct cgtcgagcag cagatattgc tggcgttcct cgaaggtcac
     1741 ctgcggtcgc gtcgtcgtga actgattggc atagaggttg tagacctcct gccggttgaa
     1801 gccgtcggtc gtcaccttct gatagacgac gcgcggggtg atcgagaggc cctcggcggg
     1861 ttcccagcgc aaggagaagc ggccgccata gcgttcgccg ctgttgacgt cctcactctc
     1921 gccgccgcct tcgcgcagcg cattgatgaa gcccgcatag cgcgtgtaat agccgaccgc
     1981 gcgcatcgcg agattctcgc tcagcggcag gttgatcgcg cctttgaggt gcccgccgaa
     2041 atcgtcgccg tcgacgagat tcacattgcc ttcgaccttg ccctcgacgc ggtcggtggt
     2101 cggctggttg gtgatgtagc ggagcgtgcc gccgaccgat cccgagccga agagcgtgcc
     2161 ctgcggtccg cgcagcgttt cgacgcggtt caaatcgaag aggtcgaggt cgggggtgaa
     2221 gagcgacagc gaaatcaccg attcgtcgag atagacgccg acctgttcct tgacccccgg
     2281 ctggtcgcgc gcgatctggc cggccgaaac gccgcgtacc gacacttggc tctgcccggg
     2341 tccgagattc tggacggtca ggcccgcgac gttgcgcgac agatcttcga tcgtgctcgc
     2401 attggcgcgt tcgatcgcct gctcggtctg ggcgttgatc gaaaagggaa cgtcctgaag
     2461 gctcgcgtcg cgcttggtcg cggtgacgat gatctcgttg ccgtcatagc tgtccgatgg
     2521 cgcgtcctgc gccgctgccg gggtgcttgc gagcgcaagg cacgacaagc ccgcgagcaa
     2581 agatgctcgt gcattcatga taatcctcct cctggtggcc aatctgacgc tggtccgggc
     2641 gggagggtgc gcctcttgcg ggtgagaggc aatgggggac gatttgcgcg caataaaatt
     2701 acccgaacct gttgcgtcgc ggccacagtc gaaaaaaggc cggggccaag ccccgggccc
     2761 gtggcccttc cggccacaat taaataaaaa aaggccggga cgtcggtccc ggccagtggt
     2821 tcgcaggagg aacctggtta ggcccccgcc gccgccgttt gaaaggagag agacgtgcgg
     2881 cgacggggtt cctgtctcgt caataattat aggcgcgctc gccatgttcg ccgaggtcga
     2941 ggccttcgcg ttcgacctct tcggagacgc ggccgccggt gaccgccttg gcgatcgtga
     3001 aggcgatggc cgaaccgacc gccgaccaga ggacggcgac gccgaccgac ttgatctgga
     3061 tccagagctg gctgccgagg acataatcgt cgcccgccgg accgccgagc gcgggcagca
     3121 tggtgaccgc ggtcagcacc gagccgatca ggccgccgat gccgtggatg ccgaacacgt
     3181 cgagcgagtc gtcgaagccg agcttgggct tgatcttcat cacgaaccag cagcagacga
     3241 tgccggcgat cgcgcccaaa aggatcgcgc cgaacggtcc cgaattgccc gcggccggag
     3301 tgacggcgac aaggccggcg atcgcgcccg agcaggcgcc gagcagcgag cccttgtgac
     3361 cgagcacgcg ctcggtgagc atccagaaga ggacgccggc ggcggtggcg gtgaaggtgt
     3421 tgatgagggc aagacccgcc gagccattgg cttcgagtgc cgagccggcg ttgaagccga
     3481 accagcccac ccacaggagg ccggtgccga tcagcgtcat ggtcagcgag tgcggcgcca
     3541 tgatgtcctt ctgatagccg cgacgcttgc cgatgatgaa gccgccgacg agcgccgcga
     3601 tacccgcgtt gatgtggacg acggtgccgc ccgcgaagtc gagcgcgccc cagccgaaaa
     3661 tcaggccggt cgaggcttcg tcaccgccca gataccagac catgtgcgcg atcggataat
     3721 agacgatggt cagccagacg atcgcgaaga ccatcaccgc cgagaatttc atgcgctcga
     3781 cgagcccgcc gaggacgagc gcgacggtga tcgccgagaa ggtcatctgg aaggcgatga
     3841 agacgaattc gggaatgacg acgccgtcgg tgaaggtcgc gacgttcgaa tcggcggtga
     3901 cgccggcaag gaagaacttg cccgacgaga tgagccagtt ggcgtcgccg ccaaaggcga
     3961 ggccatagcc gtagatgacc cacagcacca tcgcgagcgc cgcgacggcg agcacctgcg
     4021 tcagcaccga ggccatattc ttggtgcgga cgaggccgcc gtaaaagagc gcgagcccgg
     4081 gcacgatcat cgccatcacg agcacggtcg cgaccatcat ccacgcggtg tcgcccttgt
     4141 cgggcgtcgg cgcggcagct tcggcggcgg cgggcgccgc ttcctgcgcc catgcgggca
     4201 gcgc
//
LOCUS       DATMSU010000497         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_17351, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000497 DATMSU010000000
VERSION     DATMSU010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17351"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..1252
                     /locus_tag="VN034_13195"
     CDS             371..1252
                     /locus_tag="VN034_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWV61612.1"
                     /translation="MAARAYWKGQIRLALVSIPVEIYAATKSGAAISFRQIHEPSGKP
                     VKYEKVVPGIGPIDTDDIVKGYEMSKGNYVLLDEEEIEAVKLDSKRTLELLQFVDAGD
                     IDILYYAKPYYVVPADELAEEAYIVLREALKRTKKVGLGQLSVRGQEQLVGLRPCGKG
                     LVLEVLRYADEVNKAANYFRDVGNSKPDADLLDLAETLIGKKSGKFDASDYHNHYVDA
                     LKRVIAKKAKAKGKRVLEDVEEPGPVSGGSNVIDLMAALKASVEGKKKAASKAAPEKK
                     APAKKAPAKKTAERKRA"
     gene            1252..1800
                     /locus_tag="VN034_13200"
     CDS             1252..1800
                     /locus_tag="VN034_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006958353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWV61613.1"
                     /translation="MELNDALWWETIDTEVLLRLGIATLVGLLLGLDRELKGYDAGLR
                     THGMVALSSSLMTVVAIALYHQLGGADSSLDPLRVIEGMAAAIGIIAAGLIIVKGGEV
                     RNLTTAAHIWLTAAIGIASGAGYFAVVIIAAGLALVLLTLLRFVERRLPEVEDGPKDD
                     EPAAKNGARENGPKAEDGDVPR"
     gene            1787..4291
                     /gene="ligD"
                     /locus_tag="VN034_13205"
     CDS             1787..4291
                     /gene="ligD"
                     /locus_tag="VN034_13205"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HWV61614.1"
                     /translation="MSRADPLAQYNAKRDFKLTPEPAGKVEKGAGNRFIVQKHDATRL
                     HYDFRLEVDGVLKSWAVTKGPSADPADKRLAVRTEDHPMSYADFEGVIPKGEYGGGSV
                     MLWDRGSWAPIAGKSAKDIEKGHLHFTLDGERMKGEWLLVRMKPRGNEKRENWLLRKV
                     NDGFAGGTDDLVGRQLTSILTGRTMAEIAGDEGGEQSLKGAKGQAFAKKMAAAAAHNK
                     KVAKAKPKAKLPKFRPLQLATLVDAVPTGNGWFHEIKYDGYRAEIAASGGDVRVYTRN
                     GLDWTDKFAPLVRHIAALDLPPCLIDGEIVAYGRDGNPDFSSLQAVLKRGHGAQDEAT
                     RLHFFAFDLLEVDGKSLAKLGNLERKERLEALLRDAKAPVAVADHVIGAGETLYGAMC
                     GAGQEGIISKRADAAYVGRRTKSWVKVKCTRRQEFIIVGWKPSSTKARPFSSLLLAQH
                     EGGGLVYKGNVGTGFNADTMADLAKKFARLERKTAPLEVDKLAARKVHWLKPELVAEI
                     AFAEITAGGSVRHASFLGLRSDKEAKEVTPEVKQPAPEAQSEVRISSRDRVIFPEAKA
                     TKGDLADYYAAIAPLMLAHMARRPISLVRCPQGRAKKCFFQKHDSGSFGDHVHHVPIR
                     EKDGGHEDYLYVEDADGILACVQMGTIEFHGWDSHVGALEKPDRMVFDLDPDEGLDFA
                     DVKKSALDIRRQLADIGLVSFAMLSGGKGVHVVVPVDPGHGWDAHKDFSKRFAEALSL
                     AEPDRYVATMSKAKRKGKIFIDYLRNQRGSTAIMPYSARARENAPVAVPIGWDALADV
                     KGAGQWTIKDADALLERAAGADLKGWGFASQSLPDF"
BASE COUNT          881 a         1425 c         1410 g          652 t
ORIGIN      
        1 gcgctctggc ccgccgccgc cgcgcccgcc gacatcaggc ccgcgaagat gttcgccgac
       61 catgtgaagc tcaacaagga caaagggctc ggcgcgcgca tcgcgggcgc cttcgtctcg
      121 gttccggggc tgatgcagcg cgggctcgac aaggattaca aggacaatct ctactagctg
      181 tcccgtcttc ataatcgtca tcccggcgaa ggccgggatc tcgccgttgc gttagaaggc
      241 gagggtgaga ttccggcctt cgccggaatg acgaactggg taggtctcgc tccagtctga
      301 acctatcgcc cgtccgggaa cccctcgccg ccccgaacat ttggagtcgg gagcagcgaa
      361 agggagtgcc atggccgcac gcgcctattg gaagggacag atccggctcg cgctggtgtc
      421 gatcccggtc gaaatctatg ccgcgaccaa atcgggcgcc gcgatcagct ttcgccagat
      481 ccacgaaccg agcggcaagc cggtcaaata tgaaaaggtc gtgcccggca tcgggccgat
      541 cgacaccgac gacatcgtca agggctacga gatgtcgaag ggcaattatg tcctgctcga
      601 cgaggaggag atcgaggcgg tcaagctcga cagcaagcgc accctcgaac tgctccagtt
      661 cgtcgacgcg ggcgacatcg acatcctcta ttatgccaag ccctattatg tcgtccccgc
      721 cgacgaactc gccgaagagg cctatatcgt cctgcgcgaa gcgctgaagc gcacgaagaa
      781 ggtcgggctc ggccagctgt cggtgcgcgg gcaggaacag cttgtcgggc tgcgcccgtg
      841 cggcaagggt ctcgtgctcg aagtgctgcg ctacgccgac gaggtgaaca aggcggccaa
      901 ttatttccgc gatgtcggaa acagcaaacc cgacgccgac ctgctcgacc tcgccgaaac
      961 gctgatcggc aagaaatcgg gcaagttcga cgcgtcggac tatcacaatc attatgtcga
     1021 cgcgctgaag cgcgtgatcg cgaaaaaggc gaaagccaaa ggcaagcgcg tgctcgagga
     1081 tgtcgaggaa cccggacccg tcagcggcgg atcgaacgtc atcgacctga tggcggcgct
     1141 gaaagcgtcg gtcgagggca agaagaaggc cgcatccaag gccgcccccg aaaagaaagc
     1201 gccggcgaaa aaagcgccgg cgaaaaaaac cgccgagagg aaacgcgcct gatggaactc
     1261 aacgacgcgc tctggtggga aacgatcgac accgaggtgc tgctgcgcct cggtatcgcg
     1321 acgctcgtcg ggctgctgct cgggctcgac cgcgaactca agggttatga cgcggggctt
     1381 cgcacccacg gaatggtcgc gctcagttcg tcattgatga ccgtcgtcgc gatcgcgctc
     1441 tatcaccaac tcggcggcgc ggactcgtcg ctcgacccct tgcgcgtgat cgaaggcatg
     1501 gcggcggcga tcggcatcat cgccgcgggg ctgatcatcg tcaaaggcgg cgaggtgcgc
     1561 aacctcacca ccgcggcgca tatctggctg accgcggcga tcggcatcgc gagcggcgcg
     1621 ggttatttcg cggtggtgat catcgccgcc gggctcgcgc tcgtgctgct cacgctgctc
     1681 cgcttcgtcg aacgccgcct gcccgaggtc gaggacgggc cgaaggatga cgaacccgcg
     1741 gcgaagaatg gggccaggga gaatgggcca aaagcggagg acggggatgt cccgcgctga
     1801 cccgctcgcc caatataacg ccaagcgcga tttcaagctg acccccgagc ccgcgggcaa
     1861 ggtcgaaaag ggagccggca accgcttcat cgtccagaag cacgacgcga cgcggctcca
     1921 ttacgacttc cggctcgagg tcgatggcgt gctcaaaagc tgggcggtga ccaagggtcc
     1981 gagcgccgac cccgccgaca agcgcctcgc ggtgcgcacc gaggatcacc cgatgtccta
     2041 cgccgatttc gagggcgtga tccccaaggg cgaatatggc ggcggcagcg tgatgctgtg
     2101 ggaccgcgga agctgggcgc cgatcgcagg caagagcgcg aaggacatcg aaaagggcca
     2161 tctccacttc acgctcgacg gcgagcggat gaagggcgaa tggctgctcg tccgcatgaa
     2221 accgcgcggg aacgagaagc gcgagaattg gctgctccgc aaggtgaacg acggttttgc
     2281 gggcggcacc gacgatctcg tcgggcgcca gctcacgagc atcctcaccg gccgcaccat
     2341 ggccgagatc gcgggcgacg agggcggcga acaatcgctg aagggcgcga agggccaagc
     2401 cttcgcgaag aagatggccg ccgccgccgc gcacaacaaa aaggtcgcca aggcgaagcc
     2461 gaaagccaag ctccccaaat tccgcccgct ccagctcgcg acgctcgtcg atgccgtgcc
     2521 gacgggcaac ggctggttcc acgagatcaa atatgacggc taccgcgccg aaatcgccgc
     2581 atcgggcggc gacgtgcgcg tctacacccg aaacgggctc gactggaccg acaagttcgc
     2641 accgctcgtc cgccatatcg cggcgctcga tctgccgccg tgcctgatcg acggcgagat
     2701 cgtcgcttac ggcagggacg gcaatcccga cttctcctcg ctgcaggcgg tgctgaagcg
     2761 cggccacggc gcgcaggacg aggcgacccg gctccacttc ttcgccttcg acctgctcga
     2821 agtggacgga aagtcgctcg ccaagctcgg caacctcgaa cgcaaggagc gtctcgaagc
     2881 gctgctgcgc gacgccaagg cgccggtcgc ggtcgccgac catgtcatcg gtgcgggcga
     2941 aacgctttac ggcgcgatgt gcggcgcggg gcaagaaggc atcatctcga aacgcgccga
     3001 cgccgcttat gtcggccgcc gcaccaaaag ctgggtcaag gtcaaatgca cgcggcggca
     3061 ggaattcatc atcgtcggct ggaagccctc ctcgaccaag gcgcgccctt tctcgtcgct
     3121 tctgctcgca cagcacgagg gcggcgggct cgtctacaag ggcaatgtcg gcaccggctt
     3181 caacgccgac acgatggccg acctcgcgaa aaaattcgcc aggctcgaac gcaagaccgc
     3241 gccgcttgag gtcgacaaac tcgccgcgcg caaggtccat tggctcaaac ccgagctcgt
     3301 cgccgaaatc gccttcgccg aaatcaccgc gggcggatcg gtccgtcacg cgagtttcct
     3361 cggcctgcgc agcgacaagg aggcgaaaga ggtgaccccc gaagtcaaac agcccgcgcc
     3421 cgaagcgcaa agcgaggtca ggatcagcag ccgcgaccgc gtcatcttcc ccgaagccaa
     3481 ggcgaccaag ggcgacctcg ccgactatta cgccgcgatc gccccgctca tgctcgcgca
     3541 catggcgcgc cgcccgatca gcctcgtccg ctgtccgcag gggcgcgcga agaaatgctt
     3601 tttccagaag catgactcgg gctcgttcgg cgatcatgtc caccacgtcc cgatcaggga
     3661 gaaggacggc gggcatgagg attatctcta tgtcgaggat gccgacggta tcctcgcctg
     3721 cgtccagatg gggacgatcg aattccacgg ctgggacagc catgtcggcg cgctcgaaaa
     3781 gcccgaccgc atggtgttcg acctcgatcc cgacgagggg ctcgatttcg ccgacgtcaa
     3841 aaagtccgcg ctcgacatcc gccgccagct cgccgacatc gggctcgtca gcttcgcgat
     3901 gctgtcgggc ggcaagggcg tgcatgtcgt cgtgcccgtg gatccggggc atggctggga
     3961 cgcgcacaag gatttttcga aacgcttcgc cgaagcgctg agcctcgccg agcccgaccg
     4021 ctatgtcgcg acgatgagca aggcgaagcg caagggcaag atcttcatcg attatctgcg
     4081 caaccagcgc ggcagcaccg cgatcatgcc ctattcggcg cgcgcgcgcg aaaacgcccc
     4141 cgtcgcggtg ccgatcggct gggacgcgct tgccgacgtg aagggcgccg gccagtggac
     4201 gatcaaggat gccgacgcgc tgctcgaacg cgccgccggc gccgacctca agggctgggg
     4261 cttcgccagc cagtcgctcc ccgatttcta gcgcagcgag ctttaaatca gcatcccctc
     4321 ttccgttcgt gtctagcgaa gtcgagacac ccatcggcgt ggcgcaag
//
LOCUS       DATMSU010000498         5588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_150092,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000498 DATMSU010000000
VERSION     DATMSU010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150092"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1412)
                     /locus_tag="VN034_13210"
     CDS             complement(78..1412)
                     /locus_tag="VN034_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWV61615.1"
                     /translation="MAEAAAIDEAERDEARARRLRWRMLVGFLAGLAIGLAVHYGAPD
                     AAWVETITTYVTGPLGQIFLRLLFMLVIPLLVSALIVGIAEMGEMRALKSVGLRTLVY
                     TIAVSTISVVISLLVVNLLQPGAGVDPALARSLMAEGAAGAQAIADRAGESKSGMDAV
                     VAIVPDNIFAAMGANDVLAVMFFALFFGIGLMLVSTPRTEALKTAIEGVFEVVMKLIG
                     LVIQLAPIAVFCFMFNLAAQFGWDLIIRLSAFVGVVLLALALQMFVVFPLLLKTLARK
                     SPVAFFRETQEAFVMAFATASSNATLPTALRVAHDRLRLPDRVARFVLTIGATANQNG
                     TAMFEGVTVIFLAQFFGIDLTLQQQIVVMLVCILGGIGTAGVPAGSLPVIALILASVG
                     VPPEGIGLILGVDRFLDMCRTTLNVIGDLVAATVVSAGVKDGTAAQREPPAA"
     gene            complement(1461..2804)
                     /locus_tag="VN034_13215"
     CDS             complement(1461..2804)
                     /locus_tag="VN034_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HWV61616.1"
                     /translation="MDRKLTLYILAGMVLGIVVGYAVRVAVPADSQAFEYWLRSFGTL
                     ATIFLNLIKMLVAPLILGTLVTGIAHMGDSSALGRIGTRAIAWFIIASLISISLGLIM
                     VNLLQPGVGVDMAASAQSVGEVKKLDMFEFIEHIFPKNVIAAMAENNVLQILVFSLFA
                     GVGLTALGEKGKPLVRGAEALAELMLQITGYVMRLAPLAVFGALAKVVAQEGLGILAT
                     YAELLTEFYIALVLLWVLLLAAGAVFLRGRIIQLIRYIREPLLIAFSTASSEAAMPKL
                     FEQLDRFGVPRRISGFVLPLGYSFNLDGSMMYASFATIFIAQAYGIELSIGTQILILL
                     TLMVSSKGIAAVPRASLVVITATLAMFDLPVEGVALVFAIDHFLDMGRTATNVVGNAV
                     ATSVITKWEGMLEVPEPADAERPHAPSHTAHHGTRGLDLRDTDGAGDNKPAADNA"
     gene            complement(2919..4703)
                     /locus_tag="VN034_13220"
     CDS             complement(2919..4703)
                     /locus_tag="VN034_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWV61617.1"
                     /translation="MIDALRAFIEAHQAVLSLFILAAMFVGFLMERLPATVIAILGAC
                     TYLALGILDADGLYSVFSNSAPIVIGAMFVLSGALIRTGVIQRVADAIMKRAEKHPRL
                     AIVEVLVGALFASAFLNNTPVVVVLLPIMLKLAEVTGVSAKKLLMPLSIAAVLGGTLT
                     LIGTSTNLVVDGIVRQAGMERFGIFEITPVGLVTAASGMIGLALMWWLLPSDKPVKGP
                     AGTHDAHQYLTELVLGDDDDLVGMTVEAFRDLPRSGRIVGVRRGSVVVHSADLEHWTL
                     AAGDRLILRVDGATLLTWREQGRFRLGIGSGEPAEGDMVVETMIASNHPAIGQRLIDI
                     PFLQKVRARIIGVDRPAHEPGPDLASVRIRPADRLLVAGGQREVEALRDNPHLIGADL
                     AKVRAFRRGKAWIAILCMIAVVALAALDIMSIGVAAIIAIGVILVTRCIDSEEAWGTI
                     DGDVLILIFAMLAVGLALEQSGAVAMMVGWVEPLLADQPMWVLIFGIYFFALILSELL
                     SNNAVAALMTPVTLAIAAELGVDPRPLVIALMIGASACFATPIGYQTNTIVYAAGDYR
                     FVDFVKIGVPLNIITGVSTCLAIAFFIT"
     gene            complement(4970..>5588)
                     /gene="aroC"
                     /locus_tag="VN034_13225"
     CDS             complement(4970..>5588)
                     /gene="aroC"
                     /locus_tag="VN034_13225"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWV61618.1"
                     /translation="VAEIGGDAINPENFDLEEIDRNPFFCPDPAAAQRWEGLMDAARK
                     SGSSLGAVIECAASGVPAGWGAPIYAKLDSDLAAAMMGINAVKGVEIGAGFHAARLRG
                     EENADPMRPASDGSNRPDFLSNNAGGIAGGISTGQPVVVRVAFKPTSSILTPVPTVNK
                     AGEATDIVTKGRHDPCVGIRGAPVVEAMMALVLADHKLLHRAQNG"
BASE COUNT         1056 a         1843 c         1837 g          852 t
ORIGIN      
        1 cccggccgcc tcgcgccgaa cgtcctgcgc gccccggcgg acgcacgcgc atcaacagcg
       61 aacgccccga ctaagagtca ggcggccggc ggctccctct gcgccgccgt tccgtccttc
      121 accccagccg acaccaccgt cgccgcgacc agatcgccga tgacgttgag tgtcgtgcgg
      181 cacatgtcga ggaagcggtc gacgccgagg atcaagccga tcccttcggg cggcacgccg
      241 accgaggcga ggatcagcgc gatcaccggc agcgaacccg cgggcacacc cgccgtcccg
      301 atcccgccga ggatgcagac gagcatcacg acgatctgtt gctgaagcgt cagatcgatg
      361 ccgaaaaatt gcgcgaggaa gatcacggtc accccctcga acatcgcggt gccattctgg
      421 ttcgccgtcg ccccgatcgt cagcacgaag cgcgcgaccc ggtcgggcag gcgcaggcgg
      481 tcgtgcgcga cgcgcagcgc ggtcggcagc gtcgcgttcg acgaagcggt cgcaaaggcc
      541 atgacgaagg cctcctgcgt ctcgcggaag aaggcgacgg gcgattttcg cgcgagcgtc
      601 ttgagcagta gcgggaagac gacgaacatc tggagcgcga gcgcgagcag cacgaccccg
      661 acaaaggccg acaaacggat gatcaggtcc cagccgaact gcgcggcaag gttgaacatg
      721 aagcagaaga cggcgatcgg cgcgagctgg atgacgaggc cgatcagctt catcaccacc
      781 tcgaacacgc cttcgatcgc ggtcttcagc gcctcggtgc gcggggtgct caccagcatc
      841 agcccgatgc cgaagaaaag cgcgaagaac attaccgcga gcacgtcgtt cgcgcccatc
      901 gcggcgaaga tattgtcggg gacgatcgcg accaccgcgt ccatccccga tttcgattcg
      961 cccgcgcgat cggcaatcgc ctgcgccccc gccgcgccct ccgccatcag cgagcgcgcc
     1021 agcgcgggat cgaccccggc gcccggctgg agcagattga cgacgagaag gctgatgacg
     1081 accgagatcg tcgacaccgc gatcgtatag acgagcgtgc gcagcccgac cgatttcagc
     1141 gcgcgcatct cccccatttc ggcgatgccg acgatcagcg ccgagacgag cagcgggatg
     1201 acgagcatga agagcaggcg caggaatatc tgcccgagcg gccccgtcac ataggtggtg
     1261 atcgtttcga cccacgccgc gtccggcgcg ccataatgga cggcgagccc gatggcgaga
     1321 ccggcaagga agccgaccag cattcgccag cgcagccgcc gggcgcgcgc ctcgtcccgc
     1381 tcggcttcgt cgattgccgc tgcttcggcc atgcctctgc tccttgctcg cccccaatgg
     1441 ggcggccggc agctcgcgcg ctaggcgtta tcggccgctg gcttattgtc ccccgccccg
     1501 tctgtatcac gaagatcgag cccgcgggtt ccatgatgcg ccgtatgcga cggcgcgtgc
     1561 ggccgttcgg cgtcggcggg ttcgggcact tcgagcatgc cttcccattt ggtgatgacg
     1621 ctcgtcgcca ccgcgttgcc gacgacattg gtcgcggtgc ggcccatgtc gaggaaatgg
     1681 tcgatcgcga agacgagcgc gacgccctcg accggcaggt cgaacatcgc gagcgtcgcg
     1741 gtgatgacga cgaggctggc gcggggcacc gctgcgatcc ccttcgacga taccatcagc
     1801 gtcagcagga tcagtatctg ggtaccgatc gacagctcga tgccataggc ctgcgcgatg
     1861 aagatcgtcg cgaagctcgc gtacatcatc gacccgtcga ggttgaagct gtagcccagc
     1921 ggcagcacga aacccgaaat gcggcgcggg acgccgaagc ggtcgagctg ttcgaagagc
     1981 ttcggcatcg cggcttccga cgacgcggtc gaaaaggcga tgagcagcgg ttcgcgaatg
     2041 taacggatga gctggatgat gcgtccgcgt aggaagaccg cgccggcggc gagcagcagc
     2101 acccagagca gcacgagcgc gatgtagaat tcggtgagca gctcggcata ggtcgcgaga
     2161 atgcccagcc cctcctgtgc gacgaccttt gccagcgccc cgaacaccgc gaggggtgcg
     2221 aggcgcatga cataaccggt gatctgcagc atcagttcgg cgagcgcctc ggcgccgcgc
     2281 accagcggct tgcccttttc gccgagtgcg gtcagcccga cgccggcgaa aagcgagaag
     2341 acgaggatct ggagaacgtt attctccgcc atcgcggcga tgacattctt ggggaagata
     2401 tgttcgatga actcgaacat atcgagcttc ttgacctcac ccaccgactg ggcggaggcg
     2461 gccatgtcga cgccgacgcc gggctggagc aggttgacca tgatcaggcc aaggctgatc
     2521 gagatcaggc tggcgatgat gaaccaggcg atcgcgcgcg tgccgatgcg gccgagcgcc
     2581 gagctgtcgc ccatatgcgc gatgccggtg acgagcgtgc cgaggatcag cggcgcgacg
     2641 agcatcttga tgaggttgag gaagatggtc gcgagcgtgc cgaagctgcg cagccaatat
     2701 tcgaacgcct ggctgtcggc gggcacggcg acgcggaccg catatcccac cacgatcccg
     2761 agcaccatgc ccgcgagaat atataatgta agcttgcggt ccatgcggcc gtcccccgaa
     2821 tatcattatt ggcgcgccgt gaccggctgg attctccgcc ccctttgacc gaccgcaagc
     2881 cgcagcgtca aggggctggc ggttcgcccg ctatgccgtc aggttatgaa gaaggcgatc
     2941 gcgagacagg tcgaaacgcc ggtgatgatg ttgagcggca cgccgatttt cacgaaatcg
     3001 acgaagcgat agtcgcccgc ggcatagacg atcgtgttcg tctgatagcc gatcggggtc
     3061 gcgaaacagg cggacgcacc gatcatcagc gcgatcacca gcgggcgcgg atcgacgccc
     3121 agttcggcgg cgatcgcgag cgtcaccggc gtcatcagcg ccgcgaccgc attgttgctg
     3181 agcagttcgg agaggatgag cgcgaagaag tagatgccga agatcagtac ccacatcggc
     3241 tggtccgcga gcaacggctc gacccagccg accatcatcg cgaccgcgcc gctctgttcg
     3301 agcgccagcc cgaccgccag catcgcgaag atcaggatca gcacgtcgcc gtcgatcgtc
     3361 ccccacgcct cttcgctgtc gatgcagcgc gtcacgagga tcacgccgat cgcgatgatc
     3421 gcggcgaccc cgatcgacat gatatcgagc gcggcgagcg cgacgaccgc gatcatgcac
     3481 aggatcgcga tccacgcctt gccgcggcgg aacgcgcgaa ccttggcgag atcggcgccg
     3541 atcaggtgcg gattgtcgcg cagcgcctcg acctcgcgct gcccgccggc gacgagcagg
     3601 cgatcggcgg gacggatgcg aacgctcgca agatcggggc cgggctcgtg cgcggggcga
     3661 tcgacgccga tgatgcgcgc gcggaccttt tgcaggaagg gaatgtcgat cagccgctgg
     3721 ccgatcgccg gatggttaga cgcgatcatc gtttcgacca ccatgtcgcc ctcggcgggc
     3781 tcaccgctac cgatcccgag gcgaaaccgg ccctgctcgc gccatgtcaa aagcgtcgcg
     3841 ccatcgacgc gaaggatcag ccggtcgccg gccgcgagcg tccaatgttc caagtcggcg
     3901 ctatgcacga ccaccgaccc gcgacgcacc ccgacaatgc gacccgaccg gggcagatcg
     3961 cgaaacgcct cgactgtcat gccgaccaga tcgtcgtcgt cgccgagcac cagttcggtc
     4021 agatattggt gcgcgtcgtg cgtccccgcc ggccctttca cgggcttgtc cgacggcaga
     4081 agccaccaca tcagcgcgag accgatcatc cccgacgcgg cggtgacgag gccgacgggg
     4141 gtgatttcga aaatgccgaa acgctccatc ccggcctgac gcacgatgcc gtcgacgacg
     4201 agattggtcg aggttccgat gagggtcagc gtgccgccga gcaccgccgc gatcgacagc
     4261 ggcatcagca gcttcttggc gctgaccccc gtcacttcgg cgagcttcaa catgatcggc
     4321 agcagcacga ccacgacagg cgtgttgttc aggaaagccg aggcgaaaag cgcgcccacg
     4381 agcacttcga cgatggcgag ccgcgggtgc ttctccgcgc gcttcatgat cgcgtcggcg
     4441 acgcgctgga tcacgccggt gcggataagc gcgcccgaca gcacgaacat cgcgccgatg
     4501 acgatcggcg ccgagttgga aaagaccgaa tagaggccgt cggcgtcgag gatgccgagc
     4561 gcgagatagg tgcaggcgcc gaggatcgcg atcaccgtcg cgggcagccg ctccatcagg
     4621 aagccgacga acatcgcggc gagaatgaac agcgacagga ccgcctgatg cgcctcgatg
     4681 aaggctcgca gcgcgtcgat catctagggt cagaacctgc ggattgcgcg ttcgacgcgt
     4741 cgaaatggcg aagatattgg gttcgggcgg gcgcagcggg tagcatcgtt acccgcaagg
     4801 ccgcgcgagc ccaagatcga agccatttcg gcgtccctgc gggatttggc caattttatc
     4861 catggctgcg tcggcaagtc ttggaatata ttcatatgcc tgcgccttgc ctcctagccc
     4921 tgaataaaat tgactcaaac gtcgaccgtg caatccgcag gttctgaccc tagccgttct
     4981 gcgcgcgatg cagcagctta tggtcggcga ggaccagcgc catcatcgct tcgaccaccg
     5041 gcgcgccgcg gatgcccacg caggggtcgt ggcggccctt ggtgacgatg tcggtcgcct
     5101 cgcccgcctt gttgacggtc ggcaccgggg tcaggatcga actggtcggc ttgaacgcga
     5161 cgcgcacgac gaccggctgg ccggtcgaga tgccgccggc gatgccgccg gcgttgttcg
     5221 agaggaaatc ggggcggttg ctgccgtcgc tcgcgggacg catcgggtcg gcattctctt
     5281 cgccgcgcag ccgcgccgcg tggaaaccgg cgccgatctc gacgcccttc accgcgttga
     5341 tccccatcat cgcggcggcg aggtcgctgt cgagcttggc atagatcggc gcgccccagc
     5401 cggcggggac accgctcgcg gcgcattcga tcaccgcgcc cagggaactg cccgacttgc
     5461 gcgcggcgtc catcagccct tcccagcgct gcgccgcggc cgggtccggg cagaaaaagg
     5521 gattgcgatc aatctcttcg aggtcgaagt tctcagggtt aatcgcatcg ccgccgattt
     5581 cggcgacc
//
LOCUS       DATMSU010000499         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_112551,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000499 DATMSU010000000
VERSION     DATMSU010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112551"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="VN034_13230"
     CDS             <1..2044
                     /locus_tag="VN034_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWV61619.1"
                     /translation="LAQETPVPSGDTPPPAATEVASTATTPARQIYTPADFARYAPVN
                     AYDMLIQVPGFQIRDSEELRGLGQATGNVLFNGERPSNKADDMYTQLSRIPGGNVERI
                     EIVDGATLNIPGLSGQVANIVYRADNFSGQFSWKPQFRPHYTDPLFTRGDVSVRGRSG
                     EIEYEASLSNDDSSRSGAGGPTLIYDGAGNIIERRKDIWHTHFDTPKLSGRITWDPAG
                     DTVANLGGHYQRKYDRYYEDGVRTAPGLPDSIRTVRENSDSWNYEIGGDYQFGLGPGK
                     LKLIGLRRFSHEPYSQEIVTTPDGGIATGDRFMQTGDLGETIGRGEYQWKMFGGDWQL
                     SGEAAFNTLDNVASLAVLDPSGDFVDIPFEGGTGGVSEDRYEGLLSFGRKLTDKLNFQ
                     IIAGAEHSTITQTGVNGLTRSFFRPKGSISLAWAPSADFDISVKLRRRVLQLSFYDFL
                     ARAFLNDGNQNASNNDLRPQQDWTYEGEINKKLGAWGSTKLIFIYRDVEDRVDVIPIG
                     ESGEAVGNIAKAKAGAIDWTSTINLDPAGLKGVRLNTRVLLQKSSLRDPFTGEKRQWS
                     GFTDTVVELGLRHDIPASDWAWGGDLEYSHYQPNYRRNQTDKVWEGPVWASLFVENKD
                     VFGLTVRAQVSNMVNARSKRDRIVYTGVRGDSPISFIEHRDRLIGPIFAFSVRGTF"
     gene            2062..>2335
                     /locus_tag="VN034_13235"
     CDS             2062..>2335
                     /locus_tag="VN034_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HWV61620.1"
                     /translation="MTTRRIKIYFDGGCRPNPGKMETAVVTGGAAVVVRDAGIGSSQD
                     AEWLALIAALRLAQDMGLAHYILLGDAAAVIEQANGVVRARGEAAEH"
BASE COUNT          442 a          727 c          774 g          392 t
ORIGIN      
        1 gctggcgcag gaaacgcccg tgccgagcgg cgatacgccg ccgcccgccg cgaccgaggt
       61 cgcctccacc gcgaccacgc cggcgcggca gatctatacc cccgccgact tcgcgcgcta
      121 cgcgccggtc aacgcctatg acatgctgat ccaggtgccg gggttccaga tccgcgacag
      181 cgaggaactg cgcgggctgg ggcaggcgac gggcaatgtg ctgttcaacg gcgagcggcc
      241 gtcgaacaag gccgacgaca tgtatacgca gctgtcgcgt atccccggcg gcaatgtcga
      301 gcggatcgaa atcgtcgacg gcgcgacgct taacatcccc ggattgtcgg ggcaggtcgc
      361 caatatcgtc tatcgcgccg acaatttttc ggggcaattc tcgtggaagc cgcagttccg
      421 cccgcattat accgacccgc tgttcacgcg cggcgacgtg tcggtgcgcg gacgatcggg
      481 cgagatcgaa tatgaagcgt cgctgagcaa cgacgattcg tcgcgcagcg gcgcgggcgg
      541 cccgacgctc atttatgacg gcgcgggcaa tatcatcgag cggcgcaagg acatctggca
      601 cacccatttt gacacgccga agctgtcggg gcgcatcaca tgggatccgg cgggcgatac
      661 ggtcgccaat ctcggcggcc attatcagcg caaatatgac cgctattacg aggatggcgt
      721 gcgcaccgcg ccggggctgc ccgacagcat ccgcaccgtg cgcgaaaatt ccgacagctg
      781 gaattacgag atcggcggcg attaccagtt cggactgggg ccgggaaagc tgaagctgat
      841 cgggctgagg cgtttcagcc acgaacccta ttcacaggaa atcgtgacga cgcccgacgg
      901 tggcatcgcg acgggcgacc gcttcatgca gacgggcgac ctcggcgaga cgatcgggcg
      961 cggcgaatat cagtggaaga tgttcggcgg cgactggcag ctgtcgggcg aggcggcgtt
     1021 caacacgctc gacaatgtcg cgtcgctcgc ggttctcgat ccctcgggcg atttcgtcga
     1081 cattccgttc gagggcggca ccggtggggt gagcgaggat cgctatgagg ggcttttaag
     1141 ttttggccgc aagctcaccg acaagctcaa tttccagatc atcgcgggcg ccgagcattc
     1201 gacgatcacc cagaccggcg tgaatggcct gacgcgcagc ttcttccgcc ccaaggggtc
     1261 gatttcgctc gcctgggcgc cctcggccga tttcgacata tcggtcaagc tgcggcggcg
     1321 ggtcctgcaa ctctccttct atgattttct cgcgcgcgct ttcctcaacg acggcaacca
     1381 gaatgcgagc aacaacgatc tgcgcccgca gcaggactgg acctacgagg gcgagatcaa
     1441 caagaagctc ggcgcatggg ggtcgaccaa gctgatcttc atctatcgcg acgtcgagga
     1501 tcgcgtcgac gtgatcccga tcggcgaaag cggcgaggcg gtcggcaata tcgcgaaggc
     1561 caaggccggc gcgatcgact ggacctcgac catcaacctc gacccggcgg ggctgaaggg
     1621 ggtgcggctc aacacgcgcg tgctgctgca gaaaagctca ctgcgcgatc ccttcaccgg
     1681 cgagaagcgg caatggagcg gcttcaccga caccgtcgtt gaactgggcc tgcgccacga
     1741 tattcccgcg agcgactggg cgtggggcgg cgacctcgaa tattcgcact atcagcccaa
     1801 ttaccgccgc aaccagaccg acaaggtctg ggaagggccg gtgtgggcgt cgctgttcgt
     1861 cgagaacaag gatgtgttcg gactcaccgt ccgcgcccag gtttcgaaca tggtcaatgc
     1921 gcggtcgaag cgcgatcgca tcgtctatac cggggtgcgc ggcgacagcc ccatttcctt
     1981 catcgaacat cgtgaccggc tgatcggacc gatcttcgcc ttttcggtgc gcgggacatt
     2041 ctgacggagt agggctgttc gatgacgacg cgccgcatca aaatctattt cgacggcgga
     2101 tgccgaccca atccggggaa gatggaaacc gccgtcgtca ccggcggcgc ggcggtcgtc
     2161 gtgcgcgatg cggggatcgg cagcagtcag gacgccgaat ggctggcgct gatcgcggcg
     2221 ttgcggctcg cgcaggacat ggggctcgcc cattatatcc tgctcgggga tgccgcggcg
     2281 gtgatcgagc aggcgaacgg cgtcgtccgc gcccgaggcg aagcggccga gcatc
//
LOCUS       DATMSU010000500         4417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_27086, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000500 DATMSU010000000
VERSION     DATMSU010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27086"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VN034_13240"
     CDS             complement(<1..413)
                     /locus_tag="VN034_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HWV61621.1"
                     /translation="MPADWVARWNGDPLLLIGLAALALWAWRAGVRPHFWAGYGVLLL
                     LLLSPLCALSSALFSARVVHHVLLAGVAAPLLVFGLPRLTVPGAPALWAAGHALAFWF
                     WHAPDAYAAALSSDGAYWTMQIVLLGSAAGFWAALR"
     gene            574..999
                     /locus_tag="VN034_13245"
     CDS             574..999
                     /locus_tag="VN034_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HWV61622.1"
                     /translation="MASVPPAPFRLLHPLHAILLAFPIALFSAALLSDITYLNTAEIQ
                     WTNFSAWLITGGLVFGAPALLWALILLVRRHDTGRRDILVYFLLVAVMWVAALINAFQ
                     HSRDAWSSVGTTGLILSIVSTLTALAAGWVGHSGWRSAR"
     gene            996..2345
                     /locus_tag="VN034_13250"
     CDS             996..2345
                     /locus_tag="VN034_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HWV61623.1"
                     /translation="MSIRTVLAAVIAATTLAACGSGGETLDQTGATPELPKIDETLLP
                     PMKIARPQGWNGELPTVPAGFTIVPVATDLKIPRQILVLPNGDVLVAEGSGGNAPKLR
                     PKDVIAGYIKSLGKSSVKGGDRITLLRDTNGDGKPELRTTFIDKLNAPYGLAYVDGAL
                     YVANQDALLRFPYSDGQTRVAAPGEEVTKLPSRVNHHWTKSLAASPDGSKLYVGIGSN
                     SNVGERGLSIEEDRAVIWEVDPATGSNRIFVSGIRNPTALAFEPNTNMLWAVVNERDE
                     LGAELVPDYLTSVREGAFYGWPYSYWGRHIDPRVRPQKPDLVKTAVRPDYALGSHVAP
                     LGLSFVTGPGFPGYTQGAFVGEHGSWNRQNLAGYKVSFIPFANGRPAGKPKDFVTGFI
                     KDGKARGRPVGVTYDPVRGALWIADDLSNTVWRVTAPRQAGSRPPLALASPVMRPTP"
     gene            2526..>4417
                     /locus_tag="VN034_13255"
     CDS             2526..>4417
                     /locus_tag="VN034_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWV61624.1"
                     /translation="MRIAVRTVAIILLTLFAIWLILFITKGRFLKGPFERFATSQLER
                     QVDVGGDFQLYFAPINVKFYAEDIRIANPEWASEKQFFTAKKVDTRLATFPLIFGNRI
                     LRFVDLDGAHVALEWDDGNKRNSWTFGDPDRPPEPLELPRIQRAAITGSSLTYRDPLL
                     RLFADIDIDTVKATDKGIEDAIRFAGRGTMRNQPFTLSGGLTSPNETIAGGKNALTLH
                     AEGLGNKLDVSGTLPGPTELEGADLKLQARGGNLSRIFDFLGVAIPDTRAYRLTSALT
                     YEDEVWKLTGMKGLFGDSDLAGSLAVSQPKGRVHLKADLVTRSLNIIDAGPFIGYDPQ
                     RLDKMGTSGTIQTVSGRPRVLPDAPLRVEALKRFDADVRYRITKVRAESFPISKVDLT
                     LALNNGLMELKPFNFAVAGGTVKSDISIDTRPARVRTEYDVRLSPTRLGLLLAHFGTE
                     ESGTTGTVSGRIQMVGLGDSVRQSLATSNGRIAFILPQGTFWTRNIQLAELDIGTFVQ
                     KMFQDKLKKPVEINCGLVAFTVRGGVAAADPILIDTKKNVMIGRGGFSFRSEAIDMSV
                     RADGKTFSLFSGQSPVGVGGYFAAPKIDPISDELVGRAGAGLGLGVLASPIAAIIPFV
                     DPGDAQA"
BASE COUNT          791 a         1569 c         1359 g          698 t
ORIGIN      
        1 cgcagcgccg cccaaaagcc cgcggcgctg ccgagcagga cgatctgcat cgtccaatag
       61 gcgccgtcgc tcgacagcgc ggccgcatag gcatcgggcg catgccagaa ccagaaggcc
      121 agcgcgtgcc ccgccgccca cagcgctggc gctcccggaa ccgtcaggcg aggaaggccg
      181 aaaacgagca gcggcgccgc caccccggcc agcaaaacat ggtgaacgac gcgcgccgaa
      241 aaaagtgccg acgacaacgc acagagcggc gaaaggagga gcagcaacag cacgccgtaa
      301 ccggcccaaa agtgcgggcg cacgccggcg cgccacgccc agagtgcgag tgccgcgagc
      361 ccgatcagta gcagcggatc gccattccag cgggcgaccc aatccgcagg cacgggcgcc
      421 gcaccgcaat agggtatcca gggctgcgaa ggacccgcca tggaccacga acgttgcggc
      481 tggcgacagg ttccccgccc ccgatcctcg ttcgggaaac cattacgcct tcggcgcatt
      541 aacaagacca agggggcacg ccggagacgt cttatggcat cagtgccccc tgcccccttt
      601 cgcctgcttc atccgctgca cgcgatcctt ctcgcctttc cgatcgcgct gtttagcgcc
      661 gcgcttctct ccgacatcac ctatctcaac accgccgaga tccagtggac gaacttctcg
      721 gcctggctga tcaccggtgg actcgttttc ggggcccccg cgttgctctg ggcgctgatc
      781 ctgctcgtcc gtcgccacga cacgggccgc cgggacatcc tcgtctattt cctgctcgtc
      841 gcggtcatgt gggtcgcggc gctgatcaat gccttccagc acagccgcga cgcctggagt
      901 tcggtcggca cgacgggcct catcctgtcg atcgtctcga cgctgacggc gctcgcggcg
      961 ggctgggtcg ggcatagcgg ctggaggagc gcacgatgag catccggaca gtgcttgcgg
     1021 ccgtcatcgc cgcgaccacc ctcgccgcgt gcggcagcgg cggcgaaacg ctcgaccaga
     1081 ccggcgccac gcccgagctt ccgaagatcg acgagacctt gctgccgccg atgaagatcg
     1141 cgcggccgca aggctggaac ggcgagcttc ccaccgtgcc ggcgggcttc accatcgtgc
     1201 ccgtggcgac cgatctcaag atcccgcgcc agatcctcgt gctgccgaac ggcgacgtgc
     1261 tggtcgccga aggctcgggc ggcaatgcgc ccaagctgcg cccaaaggat gtgatcgcgg
     1321 gctatatcaa gagcctcggc aaaagcagcg tgaagggcgg cgaccgcatc acgctgcttc
     1381 gcgacaccaa cggcgacggc aagcccgagc ttcgcaccac cttcatcgac aagttgaacg
     1441 caccctatgg tctcgcgtac gtcgacggcg cgctctatgt ggcgaaccag gacgcgctgc
     1501 tgcgcttccc ctatagcgac gggcagacga gggtcgccgc gcccggcgag gaagtcacca
     1561 agctcccgtc cagggtcaat catcactgga ccaagtcgct cgcggcaagc cccgacggct
     1621 cgaagctcta tgtcggcatc ggttcgaaca gcaatgtcgg cgagcgcggg ttgagcatcg
     1681 aggaggaccg cgcggtgatc tgggaggtcg atcccgcgac cggcagcaac cgcatcttcg
     1741 tgtccggcat ccgcaacccg accgcgctcg ctttcgagcc caataccaac atgctctggg
     1801 cggtcgtcaa cgaacgcgac gaactcggcg ccgaactcgt ccccgattac ctcacctcgg
     1861 tacgcgaagg cgccttttac ggctggcctt acagctattg ggggcgacat atcgacccgc
     1921 gcgtgcggcc gcaaaagccc gacctcgtca aaaccgcggt ccggcccgac tatgcgctcg
     1981 gctcgcacgt cgccccgctc ggtttgagct tcgtcaccgg accgggcttc cccggctata
     2041 cgcaaggcgc cttcgtcggc gagcatggca gctggaaccg ccagaacctc gcgggataca
     2101 aggtgagctt catccccttc gcgaacggcc ggcccgcggg caagcccaag gatttcgtga
     2161 ccggcttcat caaggacggc aaggcgcgcg gccggcccgt cggcgtgacc tatgatcccg
     2221 ttcgcggcgc gctctggatc gccgacgatc tttcgaacac tgtctggcgc gtcacggcgc
     2281 cgcggcaggc cggttcccgc ccgccgctcg cgttggcctc gccggtgatg cgtcccaccc
     2341 cttgatcgct ggggatcacc ccagcggcgg gaacgatgcg cggaccggcg aatttctctt
     2401 ccatggatat gcgctcaggt cagacggcct ccgcggccac cagcccggca tccgcatggg
     2461 tcgcgccccg gcgctgggca cgcacggcgt ggactcctgc ctggctcgag aagcgcccga
     2521 gaccggtgcg catcgccgtg cgcacggtcg cgatcatcct cctcacgctt ttcgcgatct
     2581 ggctgatcct gttcatcacc aagggtcgct ttctgaaagg cccgttcgaa cgcttcgcga
     2641 cctcgcagct cgaacgccag gtcgacgtcg gcggcgactt ccagctctat ttcgcgccga
     2701 tcaacgtcaa attctacgcc gaggacatcc gcatcgcgaa ccccgagtgg gcaagcgaga
     2761 agcaattttt caccgccaaa aaggtcgaca cgcgcctcgc caccttcccg ctgatcttcg
     2821 gcaaccggat cttgcgcttc gtcgatctcg acggcgcgca tgtcgcgctc gaatgggacg
     2881 acggcaacaa gcgcaacagc tggaccttcg gcgatcccga ccggccgccg gagccgctcg
     2941 agctgccgcg aattcagcgc gccgcgatca ccggcagcag cctcacctac cgcgatcccc
     3001 tgctgcgcct cttcgccgat atcgacatcg atactgtgaa ggcgaccgac aagggcatcg
     3061 aggacgccat ccgcttcgcg ggccgcggca cgatgcgcaa tcagcccttc accctgtcgg
     3121 gcggcctcac ctcgcccaac gaaacgattg ccgggggcaa gaatgcgctg accttgcacg
     3181 ccgaaggcct cggtaacaaa ctcgacgtat cgggcacgct gccaggccca accgaactcg
     3241 aaggcgccga cctcaaactc caggcgcgcg gcggcaatct gtcgcggatc ttcgattttc
     3301 tgggcgtcgc gatccccgac acgcgcgcct atcgcctgac ttcggcgctg acctatgagg
     3361 atgaggtctg gaagctcacg ggcatgaaag gcctgttcgg cgacagcgac ctcgcgggtt
     3421 cgctcgcggt gagccagccg aagggccgcg tccatctcaa ggccgatctc gtcacccgct
     3481 cgctcaacat catcgatgcc ggtccgttta tcggctatga tccgcagcgg ctcgacaaga
     3541 tggggacgag cggcaccatc cagacggtca gcggccgccc gcgcgtgctg cccgacgccc
     3601 cgctgcgcgt cgaggcgctg aaacgcttcg acgccgacgt gcgctaccgc atcaccaagg
     3661 tgcgcgccga aagcttccca atcagcaagg tcgatctcac gctcgcgctc aacaacgggc
     3721 tgatggaatt gaagccgttc aacttcgccg tcgcgggcgg caccgtgaag agcgacattt
     3781 cgatcgacac acggccggcg cgcgtccgca ccgaatatga cgttcgcttg tcgccgacgc
     3841 ggctcgggct gttgctcgcg cattttggaa ccgaagagtc gggaaccact ggcacggtca
     3901 gcggccgcat ccagatggtc gggctcggcg acagcgtgcg ccagtcgctc gccacatcga
     3961 acggccgcat cgccttcatc ctgccgcaag gcaccttctg gacgcgcaac atccagctcg
     4021 ctgaactcga catcggtact ttcgtccaga agatgttcca ggataaattg aagaagccgg
     4081 tcgagatcaa ttgcggcctc gtcgcgttca ccgtgcgcgg cggcgtcgcg gccgccgacc
     4141 cgatcctgat cgatacgaag aagaatgtga tgatcggccg cggcggcttt tccttccgca
     4201 gcgaggcgat cgatatgtcg gtccgcgccg acggcaagac cttcagcctc ttctcgggcc
     4261 agtcgccggt cggcgtcggc ggctatttcg cggccccgaa aatcgacccg atcagcgacg
     4321 agctcgtcgg gcgcgcaggc gcgggcctcg gcctaggggt attggcgagc ccgatcgcgg
     4381 cgatcatccc cttcgtcgac cccggcgacg cccaagc
//
LOCUS       DATMSU010000501         3899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_55973, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000501 DATMSU010000000
VERSION     DATMSU010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55973"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VN034_13260"
     CDS             complement(<1..1061)
                     /locus_tag="VN034_13260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV61625.1"
                     /translation="MEQVDVAIIGGGLVGCAAAWRLAARGASVALLEAGDINSGSSGQ
                     NAGSLHFQIERRFLENGDALADQAADIVALSRIAVDDWRAIEGDLDADLHVSMQGGLM
                     VAETAEEAALLERKAAREHREGLSVRTIDGDEARTIAPYLSPGVLAANFSSDEGHADP
                     RAVTPALAAAARAAGASIRTASRVQSIARENGRFTIRGERLDLSADRLLVTAGAWSAN
                     ICALLNIHMPLYPVALLMNATERTRPVVPHLIQHVGRRLSLKQTYAGNILIGGGWPSR
                     LAQTQADGFDLSARPAMIPGSLTGNLRVAVDIVPDVARLNLIRSWTGVTAISADQLPV
                     VGEVPRMPGLWVAAGGSAFT"
     gene            complement(1068..1988)
                     /locus_tag="VN034_13265"
     CDS             complement(1068..1988)
                     /locus_tag="VN034_13265"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWV61626.1"
                     /translation="MNKRSRADWQGYIPAITTPFDANRNLDLPALGVLLEWLHSEGMH
                     GLLLAGTTGEWPSMSTEECKMLFSAAGAQLGGKLPLLAGCNAYTASRVLELADHAAAS
                     GFDGIVVTPPPYYRPSADEIHGFYADISVGTKLPICVYNWPPGTGIDMPLALLERLAA
                     LDNVVAIKQSTSDLRRFVDTFFALKDEIRIFGHSMDEHGLALLEARGGDGTMGAGAVL
                     GRTHPDFYNKLWAGDIEGARECGRMDRVILDEWYTPELVGRFGSGPAILKAGLNAQGL
                     PGGHVRPPLRDVSAEDAERIRETLVALGRI"
     gene            2147..3043
                     /locus_tag="VN034_13270"
     CDS             2147..3043
                     /locus_tag="VN034_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWV61627.1"
                     /translation="MWDNALQYFYEAANLGSMRLASDKIGVAVSSISRQIAQLESEMG
                     IALIERGRRSIRLTEAGQLAFEYHKNQLADREALLNQIQELREVKSGRIDLAIGEGFF
                     GPPFTALIEGFQRRNPGIAITVTSCASSEIIRMVLDDEAHIGMILHTTSEPKIRTRSA
                     VAQPLMVLCAPGHPAAAMEALTIADISGFDLCLPPQGFRIRGTLNHAEKRAQIWLEPR
                     LVTTSIQMMREIAKAGRALTVLPRISALAELEEGSLVARPLLDDAIEHSTVSLIHRLG
                     RQLEGAPARLMSVLEARLKSWS"
     gene            complement(3056..>3899)
                     /locus_tag="VN034_13275"
     CDS             complement(3056..>3899)
                     /locus_tag="VN034_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWV61628.1"
                     /translation="VGAASMLDVYNMAQDQFPGIGRLPGLQHGAGLSTVWLMTAALNV
                     ALGNWILPHLFQLCFSAGSEATIRRNAIWQPIYSLSYFFIILLGFAALLAGTQPEGGD
                     TNAVLLQFVADRYPAWAVGIFAGTACLLALVPGSVLLLTAGSIFSRNILLPMRPGLSD
                     RASLLASRLSMIVFAAIAVWLTLGGSKSLVEIGLSAYAAIGMLAPGVYFAFTWKRAHA
                     AGIFAGIVAGYLALLLPQAQQLWTILLPGWDHGLVAMAVNAAVAALACAAWPRRAAAS
                     LHPA"
BASE COUNT          702 a         1266 c         1307 g          624 t
ORIGIN      
        1 gtgaaggccg acccgcccgc cgcgacccac aggccgggca ttctcggcac ttcgccgacc
       61 acgggcaact ggtcggcgga aatggcggtg accccggtcc agctgcggat caggttcagc
      121 cgcgcgacat cgggcacgat atcgaccgcg acacgtagat tgccggtcag tgatccgggg
      181 atcatcgcgg gccttgccga caggtcgaat ccgtccgcct gcgtctgggc cagccgcgaa
      241 ggccagccgc cgccgatcag gatattgccg gcataagtct gcttcagcga cagtcggcgc
      301 ccgacatgct ggatgaggtg cggtaccacg gggcgcgtcc gctccgttgc gttcatcaac
      361 agagcgacgg gataaagcgg catgtggata ttgagcagtg cgcagatatt cgcgctccat
      421 gcgcctgcgg tgacgagcag ccggtcggcg gacaggtcga gccgttcgcc ccggatcgta
      481 aagcgcccat tctcgcgtgc gatcgactgc acgcgcgagg cggtgcggat ggatgcgcct
      541 gcggcgcgcg cggctgcggc gagcgcgggg gtgacggcgc gcggatcggc gtggccttcg
      601 tcgctcgaga aattcgcggc gaggacgccc ggcgagagat agggggcgat cgtgcgcgct
      661 tcgtcgccgt cgatggtgcg gaccgacagc ccttcgcggt gttcgcgcgc cgccttgcgt
      721 tcaagcagcg cggcttcttc ggcggtttcg gcgaccatca gcccgccttg catcgacacg
      781 tggagatcgg cgtccagatc cccttcgatc gcgcgccagt cgtcgaccgc gatgcggctc
      841 aacgcgacga tgtcggccgc ctggtccgcg agtgcgtcgc cattttcgag aaaacggcgc
      901 tcgatctgga agtgcagcga tccggcattc tgacccgatg aaccgctgtt gatgtcgccc
      961 gcctcgagca gcgcgaccga tgcgccgcgc gccgcgaggc gccacgcggc tgcgcagccg
     1021 accagccccc cgccgatgat cgcgacatca acctgttcca taaagcctta gatgcgtccg
     1081 agcgcgacca aagtctccct gattctctcg gcgtcttcgg cggaaacgtc gcgcagtggc
     1141 gggcggacat gaccgccggg caggccctgg gcgttgaggc cggccttcaa tatggcgggt
     1201 cccgatccaa aacggccgac gagttcgggg gtgtaccatt cgtcgaggat gacgcggtcc
     1261 atgcgcccgc attcgcgcgc gccttcgata tcgcccgccc acagcttgtt gtaaaaatcg
     1321 ggatgggtgc gcccgagcac cgcgcccgcg cccatcgtcc cgtcgccgcc gcgcgcttcg
     1381 agcagcgcga ggccatgctc gtccatcgag tgaccgaaaa tccgaatttc gtccttgagc
     1441 gcgaaaaagg tatcgacgaa gcggcgcagg tcgctggtcg attgcttgat cgcaacgaca
     1501 ttgtcgagcg ccgcgagccg ttcgagcagc gcgagcggca tgtcgatccc ggtgcctggg
     1561 ggccagttgt acacgcagat cggcagcttg gtcccgaccg aaatatcggc atagaacccg
     1621 tgaatctcgt ctgccgaggg ccggtagtag gggggcgggg tcacgacaat gccgtcgaat
     1681 ccgctggccg cggcatgatc tgccagttcg agcacccggc ttgcggtata ggcgttgcat
     1741 cccgccagca gcggcagctt gccgccgagt tgcgcgcccg ccgccgaaaa gagcatcttg
     1801 cactcttcgg tcgacatgct gggccattcg cccgtcgtgc cagccagcag caggccgtgc
     1861 atcccttccg aatggagcca ttcgagcagg acaccaagcg ccggcaggtc gagattgcgg
     1921 ttcgcgtcga agggcgtcgt aatggccggg atatagcctt gccagtcggc acgcgatctc
     1981 ttgttcatgt aattcggctc ccccttggtc gggatataat caatgcggcc agccccgata
     2041 agccaaggtg cattcgcggc aagggcatca aaagacgaaa ttagttgcgt ttatcgcaac
     2101 gatcggcatt tttgggcttc gaccggcggc tttgggagga ttgccaatgt gggataatgc
     2161 gctccaatat ttctacgaag ccgcgaacct tgggtcgatg cggctggcga gcgacaagat
     2221 cggcgtggcc gtgtcctcga tcagccgcca gatcgcgcag ctcgaaagcg agatggggat
     2281 tgcgctgatc gaacgcgggc gtcggtcgat ccggctgacc gaggcgggcc aattggcgtt
     2341 cgaatatcac aagaaccagc ttgccgaccg cgaagcgctg ctgaaccaga tccaggaact
     2401 gcgcgaggtc aaatcgggcc gcatcgacct cgcgatcggc gagggcttct tcgggccgcc
     2461 ctttaccgcg ctgatcgagg gattccagcg ccgcaatccg ggcatcgcga tcaccgtgac
     2521 gagctgcgcc agttcggaga tcatccgcat ggtgctcgac gacgaggcgc atatcggcat
     2581 gatcctccac accaccagcg agcccaagat ccgcacacgc agcgcggtcg cgcagccttt
     2641 gatggtgctg tgcgcgccgg gccatcccgc cgcggcgatg gaagcgctga cgatcgcaga
     2701 tatcagcggc ttcgacctgt gcctgccgcc gcagggtttc cgcattcgcg gcacgctcaa
     2761 ccatgccgaa aagcgcgcgc aaatctggct cgagccgcgg ctggtcacga cgtcgatcca
     2821 gatgatgcgc gagattgcga aggccggtcg cgcgttgacc gtgctgccgc gcatctcggc
     2881 gctcgcggag ctcgaagaag gaagcctcgt cgcacgcccg ctgctcgacg atgcgatcga
     2941 gcacagcacg gtcagcctga tccaccggct cggccgccaa ctcgagggcg cgcctgcgcg
     3001 gctgatgagc gtactcgagg cgcggctgaa aagctggagc tgatcgcgat gtcgctcagg
     3061 cgggatgaag gcttgctgcg gcgcggcgcg gccatgccgc gcacgccagc gccgcgacgg
     3121 cggcgttgac cgccatcgcg accagcccat ggtcccagcc gggcagcagt atcgtccaca
     3181 gttgctgcgc ctggggcagc aacagggcca gatagccggc cacgattccc gcgaagatcc
     3241 ccgccgcgtg cgcgcgcttc caggtgaagg cgaaatagac gcccggcgcg agcatgccga
     3301 tcgcggcata ggccgacagc ccgatctcga cgagcgactt cgatccgccg agcgtcagcc
     3361 agaccgcgat ggcggcaaag acgatcatcg acaggcgcga tgcgaggagc gacgcgcggt
     3421 cggagaggcc tggccgcatc ggcagcagga tgttgcggct gaagatcgat ccggcggtca
     3481 gcagcagcac cgaccccggc accagcgcaa gcaggcatgc cgtccccgcg aagatgccga
     3541 ccgcccacgc cggatagcga tcggcgacga actgcagcag cacggcgttg gtgtcgccgc
     3601 cttcgggctg cgttccggcg agcagtgccg cgaaaccgag caggatgatg aagaaatagg
     3661 atagcgaata gatcggctgc cagatcgcgt tgcgccggat cgtcgcttcg cttcccgccg
     3721 aaaaacagag ctggaacagg tgcgggagaa tccagttgcc gagcgcgacg ttgagcgccg
     3781 cggtcatcag ccagaccgtc gacaggccgg ctccgtgctg gagccccggc agccggccga
     3841 tgccgggaaa ctggtcctgc gccatattat agacgtcgag catcgacgcc gcgccgacc
//
LOCUS       DATMSU010000502        15159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_122383,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000502 DATMSU010000000
VERSION     DATMSU010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122383"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VN034_13280"
     CDS             complement(<1..606)
                     /locus_tag="VN034_13280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61629.1"
                     /translation="MSAIMAEAPAKLDIPAKAGVSKARAGYILFILCLISALNYYDRF
                     LIGILVEDLKRDLALSDAQVGLLSGLAFALVYSIASIPVASYADRGFRVRVLGGAAAI
                     WSVMTGLCGMATGFWTMLFARFGVGIGESGGAPTTHAIVAETFGDRWRATALAAIGVA
                     GAIGTSAAFAGGGYIAQHYGWRWAFFAAAIPGVVIALIFALT"
     gene            complement(603..2312)
                     /locus_tag="VN034_13285"
     CDS             complement(603..2312)
                     /locus_tag="VN034_13285"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWV61630.1"
                     /translation="MTSPSLALDALAQRYWDFRCDEFPTEAILAGLTPRSDLLLRDAP
                     DDHERRAAWASAALEELNAIDFDALSQTERATFMLLRGELAQTVDIVAQQGHLRPSIY
                     PLGPEFGLASWASMTSIATLEEARRYIDRLAQVPAALEGVQQSLTEGRERGIRYPKLV
                     VERAVGVVKGQAAVDVEANSFFGPLKVLATRGAAFADIVEEGRTIVANTVVPALMNYA
                     LFLEKVLLPVARDALDCVGDVGGEGHYRFLIRQYTTIDLPPEDIHAIGLAEVERITGE
                     MEAVAADGGFAGDLVGFKRQLQGDNSQLLESGEALRQEIEILSKRIDARIPEFFGRIP
                     RATYGVKSIPEAIAAAMPPAYAQPNPADNSAAGVHWITSIPSKLPRYMHLPIALHEAW
                     PGHLMHLALIQELTDLPAFRRHGALHYSACLEGWALYSEGLGEDMGLYDTPEKRYGRL
                     EMEMWRAVRLVVDTGLHAKRWTRSQAIDYFRENMAMPIETVEAEVDRYVGLPGQALGY
                     QLGNMKFRELRARAEAALGARFDIRAFNDALASAGAVSLPVLEQTIDDWLEERKADAR
                     IAA"
     gene            complement(2318..4021)
                     /locus_tag="VN034_13290"
     CDS             complement(2318..4021)
                     /locus_tag="VN034_13290"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWV61631.1"
                     /translation="MADLSNYIVNPGPKTPVTGLKAAVTTDSAIVTETIMKVLNSGGN
                     AADAVIAGAMVQAAVEPFMTNHAGLVTMLYYEAKTGKIHQLDSLGGHPSGLPPFKPVP
                     PGLGSYATSPPSAIIPGFMPGLKEIHAKFATKDWADLCADAVRWAETGHEVSTFEYGV
                     NIYGEKFITYFPEGRDFYQPNGYFPNVGDVFTPPGMAETLRGVRDNGPDHMITGAWAE
                     KFVAKGNDLGWKVKLEHMTETPPRWAEPLRFAYNEYEILFLGPPQAQGFFSSVALGVL
                     KHLGIRDMAPGSAEHIWAMGHALRQGQRHWEYARDDHVYDVPRAEVLDDDYHKHLAKL
                     IRGSRPKVDMTDHIRLSGDGPGGAGDMMAAIAGPSGKPRLVHHEEKQPSGSCEIAVVD
                     AEGNWCQFMDTLQGSGIPGQVVGGIPMVGSHSTFGALQSPMDTVLIKGAKARCIIGNT
                     LVLKDGKPVFSAGSPGNIHCTLPQVLAYLLDFKLDPYAAVDAPRMLPMTDGQLVAIED
                     RLAPGVVQDLHKLGVRVGGLSGYDFHMGSFSVIARDEASGTLTAVADPRRCAVADGIK
                     E"
     gene            complement(4057..4899)
                     /gene="purU"
                     /locus_tag="VN034_13295"
     CDS             complement(4057..4899)
                     /gene="purU"
                     /locus_tag="VN034_13295"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWV61632.1"
                     /translation="MTTSNLVLKFRAPDKPGIMATVAPTLAARGCDIREAEVYGDDDT
                     CGFFLRMALTSPVGRDELAKVVEPVATELALEWELHDLARRTPVLLAVSKFGHCLIDI
                     IHKKEIGQLPIDIVGVVSNHETMRKTAEWHGLAYHHLPMSEGKETQEARFLSIIEESG
                     AELTVLARYMQILSDGFSEALAGRCINIHHSFLPSFKGAKPYHQAHGRGVKIIGATAH
                     FVTADLDEGPIIEQDVRRVSHRTTADEMVAIGRDIEASVLSRAVRWYAEHRIFQNGNK
                     TVVL"
     gene            complement(4896..5474)
                     /locus_tag="VN034_13300"
     CDS             complement(4896..5474)
                     /locus_tag="VN034_13300"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HWV61633.1"
                     /translation="MADVGTMSLAPAAAKDLLVEGAGVRFAAETQLGIAKLQILAADP
                     DAWFEQALGLAPPPALTEIEPGPIAIAWLAPGEWLVTGDAADVERVRLRCAEAAGNLG
                     LMVDVTHGRATFELSGADARSVLATHCPLDLGDEAMPVGAAKRSMFSDTGFFISRRSD
                     RDGQPCFRLIFDQTMAGYAERMLGATIAGAVL"
     gene            complement(5467..8394)
                     /locus_tag="VN034_13305"
     CDS             complement(5467..8394)
                     /locus_tag="VN034_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HWV61634.1"
                     /translation="MSGWRLGTAGRVDRGKQVAFRWAGRTLHGYAGDTLASALMANGV
                     SIVGRSFKYHRPRGLLAAGLEEPNAIVQLETGAAAVPNVKATQIELYEGLVANPVNAK
                     PSVEFDLLAVNSLFKPFIPAAFYYKTFMWPNWHWFEPAIRKAAGLGDAPEESDPDSYD
                     HRFAHTDIVVVGSGAAGLAAAIAAAKGGEQVLLVEADAELGGGLLAEPSEIEGVAPLA
                     WRDRMLVALAGLPNVTVMNRTMAFGFYDHQLVGLCERVTDHLPPQARSGPRQRLWKVR
                     TKRVILATGAFERPIAFAGNDLPGVMLASAAQTYALRYGVAPGRRIVVATNNDSAYHA
                     AFALHDAGVVIAAIVDSRTKGGNAVAGAMARGIRTIMDSAPIKAKGRRRVKGLTLGKI
                     ADGKASETIACDTILMSNGWNPAVHLHSQSGGSLAFNSSLEAFLPSKSVQAAVNVGAA
                     AGIFDLEIALASGRAAGQGDEVGAPFPAAVGPTLHFADGDMRAAKAWVDFQNDVTAGD
                     VQIAARENFRSVEHLKRYTTLGMASDQGKTSNVTGIHMLASLLGKPPQAIGTTKFRPP
                     FDPVTIGGFAGRAVGENLMPIAHSAAHASALAAGAKMENYGSWLRPAYFPRTGEDEHA
                     AVAREVLAVRGQVGLFDASPLGKIEVKGPDAAEFLQRIYVNGVRNLKVGRCRYGLMLS
                     EHGIVYDDGVFAKIAEDHFLVGTTSGHAAAIADMLQEWLQCEWTDLKVLVENVTTAWA
                     VMNVAGPKARAVLEGIGTDIDLSREAFPHMSYREGRVGGVPARIQRVSFSGELSYEVS
                     VPWGYGRALWDAMMQAGAPHGITPFGVEALMALRIEKGFLHVGSDTDGTTMPQDVGFG
                     VAVSKKPDDFIGRRSTMTPEGLRGDRRQLVGLETLDAGGAFECGAHIVAADAKGIGAT
                     QGWVTSSVQSPTLGRPLAMALIERGTARIGETVRVWNLGKGRPARIVDPRFYDPAGGK
                     LDG"
     gene            complement(8391..8720)
                     /locus_tag="VN034_13310"
     CDS             complement(8391..8720)
                     /locus_tag="VN034_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HWV61635.1"
                     /translation="MLLIDCPFCGPRAEIEFRCGGESHIQRPGPHDRVSDAEWADYLF
                     YRENPKGEHRERWFHAAGCRRWFNAARDTVTHRFRVTYKMGDACPETGESAVSEPAPV
                     AAQGGIV"
     gene            complement(8736..9983)
                     /locus_tag="VN034_13315"
     CDS             complement(8736..9983)
                     /locus_tag="VN034_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HWV61636.1"
                     /translation="MTRRYSALSLLREGLNGQRGWEPAWRDPTPKTHYDAIVIGGGGH
                     GLATAYYLAKNHGLTNVALIERGWIGGGNTGRNTTIIRSNYFFPESAAFYEFSVQLYE
                     KLTDELNYNIMFSQRGMWTLAHDRHGVEMARRSANAMLLNGIDAEYHDGPSVYANIPG
                     LNPNPRFPILGGVNQPRGGSARHDAVAWGYARAASALGVDIIQNCEVQDFIIEGGQVR
                     GIQTSRGTIRSDRIGMAVAGHSSVLAAKAGLRLPISSYALQAFVSEPVKPCLDTVILS
                     PATGTYLSQSDKGELVLGAGLDLYLSYAQRGNLPWMERAAAGVLELFPQFSRMRFLRQ
                     WAGIVDVVHDSTPIIGPTSIGGLYINGGWGTGGFKAVPAGGWCLAHVMATGKSHPLAS
                     AFGLERFETGAMIDEASASGIAH"
     gene            10444..11316
                     /gene="folD"
                     /locus_tag="VN034_13320"
                     /pseudo
     CDS             10444..11316
                     /gene="folD"
                     /locus_tag="VN034_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
     gene            11835..14084
                     /locus_tag="VN034_13325"
     CDS             11835..14084
                     /locus_tag="VN034_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61637.1"
                     /translation="MQHRNLWFATAAIGALLTATGAQAQSAGEDPASTADGQYHGAEI
                     VVTASKREERLRDVPTAITAIGSDAIETLGIRDFRDYATLTPGLSQRDFGAPGTGTVI
                     LRGLNTGPQQTTNTSAFYIDDAPYSSNGFLSVGSLVTPSPDLVDVERIEVLKGPQGTL
                     YGASNLGGLVRVITKKADTAAFSGMAQVEGETLAHGNEGWAVRGAVNLPVVTDKLAIR
                     VSGFYRKTAGFTDNVETGENNVNSSTIKGGRIALRATPTERLTIDVTGFYQDIENDGS
                     SGQDNVTGTTRPLFGKYKFSAVEGTGGSKIQYRSVGGTASYETDAGAIVAVANYVKTQ
                     SAISSDYTDVYGSYLSLLGIVPPGTQTIGDLGPNMKKFSAELRFVSERIGAFEFVAGG
                     FYTNEKSRYPFIITARDATGTPLPAPFDMLFRSTSFANYEEYAAFGNLTFYLTDRLDV
                     TGGIRYAKNDQSSSGPGGILFYAPTPPTSTSFSDDATTYLATIRYRPSDNVSLYARAA
                     SGYRPGGPQNSTLLPPTAQTVIRADTVWNYEAGIKATALDGALSANLAVYHIDWKDIQ
                     LNTLFGGVTLQGNAGSAKVDGFELELQARPSRNFVFGANVGHTNARISKIDPVAAASL
                     GAQSGDKLPLTPSWTVAAFADQKIPFSDSVKGSIGATIRLQSDMPSSYPGYALNPNIK
                     IPSITTVDLRAGLEMSPVSLQLRVENLFDKLGYTSLATNALYVGQPVPTNATVTRPRA
                     FILSATANF"
     gene            14102..15106
                     /locus_tag="VN034_13330"
     CDS             14102..15106
                     /locus_tag="VN034_13330"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWV61638.1"
                     /translation="MTRKIDRRAALLLAGGLAATTAAPAMAKTPKAAPGTFQPMPMPY
                     ASMFPRTKYFEIDSARAGARYAVWVSVPEVYDKDPSRSFPAVFAPDGNHFVAFSTTLC
                     ELGQWDLIDKYQSTIQVCIGYTGKDADVSLAVRARDLLPPKEPLPPGMVDAMRNGGAS
                     DLLDKAGIELYIHNLENPAADRFLAFLSEELYPFIAKNYRIQSDNLGLFGHSYGGLFT
                     TYAALQPKTIFRNFGASSPGILPGASTVFKLHADAAAAGGLSERNLHLTVGTRELTVP
                     GLYQYMVGAGSTEFIRTAGTTPVKGLSFTSKLIEDESHMTLAHPAAFDFMRQYYRRQA
                     "
BASE COUNT         2735 a         5074 c         4731 g         2619 t
ORIGIN      
        1 ggtcagcgcg aagatcagcg cgatcaccac cccgggaatg gcggccgcga agaaggccca
       61 gcgccagcca taatgctgcg cgatatagcc gccccccgcg aaggcggccg acgtgccgat
      121 cgcccctgcg accccgatcg cggcgagcgc ggttgcgcgc catctgtcgc cgaacgtctc
      181 ggcgacgatc gcatgcgtcg tcggtgcgcc gccggactcg ccgatgccga cgccgaaacg
      241 cgcaaagagc atcgtccaga agcccgtcgc catcccgcaa aggccggtca tcaccgacca
      301 tatcgccgcc gcgccgccga gcacccgcac gcgaaagccg cggtcggcgt aggaagcaac
      361 cgggatcgag gcgatcgaat agacgagcgc gaaagcgagc cccgacaaca gcccgacttg
      421 ggcatcgctg agcgcgaggt cgcgcttcag atcttcgacg aggatgccga tcagaaagcg
      481 gtcataatag ttgagcgccg agatcaggca caggatgaaa aggatatagc ccgcgcgggc
      541 cttggaaacg ccggctttgg ctggaatgtc cagcttggcc ggcgcctctg ccatgatcgc
      601 gctcatgccg cgatcctggc atccgccttt cgttcttcca gccagtcgtc gatcgtctgc
      661 tcgaggaccg gcaggctcac cgcgcccgcc gacgcgagcg cgtcgttgaa cgcgcgaatg
      721 tcgaaacgcg caccgagcgc cgcttcggcg cgcgcgcgca gctcgcggaa cttcatattg
      781 ccgagctgat agccgagcgc ctgccccggc aagccgacat agcgatcgac ctcggcctcg
      841 accgtttcga tcggcatcgc catattttcg cgaaaatagt cgatcgcctg cgagcgcgtc
      901 caccgcttgg cgtgcagccc ggtgtcgacg acaaggcgca ccgcgcgcca catctccatc
      961 tcgagccggc cgtaacgctt ttcgggcgtg tcatagaggc ccatgtcctc gcccagcccc
     1021 tcgctgtaga gcgcccagcc ttcgaggcac gccgaataat gcagcgcgcc gtggcggcgg
     1081 aaggccggca ggtcggtcag ttcctggatc agcgcgaggt gcatcaaatg ccccggccac
     1141 gcttcgtgca gcgcgatcgg caggtgcata tagcgcggca gcttcgacgg aatcgaggtg
     1201 atccagtgga cccccgcggc gctgttgtcg gcgggattgg gctgcgcata ggcgggtggc
     1261 atcgcggcgg cgatcgcttc gggaatgctc ttcacgccat aggtcgcgcg cgggatgcgg
     1321 ccgaagaatt cgggaatgcg cgcgtcgatg cgcttcgaca atatctcgat ttcctgccgc
     1381 agcgcctcgc cgctttcgag aagctggctg ttgtcgccct gcagctgccg cttgaacccc
     1441 acgaggtcgc ccgcaaagcc gccatcggcc gcaaccgcct ccatctcgcc cgtgatccgc
     1501 tcgacctcgg caagaccgat cgcgtggatg tcctcgggcg gcaggtcgat cgtcgtatat
     1561 tggcggatca ggaagcggta atgaccctcg ccaccgacat cgccgacgca atcgagcgcg
     1621 tcgcgcgcga ccggaagcag aaccttctcg agaaagagcg cataattcat cagcgccggc
     1681 acgacggtgt tcgcgacgat ggtccgcccc tcctcgacga tgtcggcgaa tgccgcgccg
     1741 cgggtcgcga gcaccttgag cggaccgaag aaactattgg cctcgacatc caccgccgcc
     1801 tgtcccttaa ccacgccaac cgcgcgttcg acgacgagct tcggatagcg gattccgcgc
     1861 tcgcggcctt cggtcagcga ttgctggacg ccttcgagcg ccgccggtac ctgggcaagg
     1921 cggtcgatat agcggcgcgc ctcttcgagc gtcgcgatgc tcgtcatcga tgcccagctc
     1981 gcaagcccga attcggggcc gagcggatag atgctgggac gcaaatgccc ctgctgcgcg
     2041 acgatgtcga cggtctgcgc aagctcgccg cgcagaagca tgaaggtcgc gcgttcggtc
     2101 tgtgacagcg catcgaaatc gatcgcattc agttcttcga gcgccgccga cgcccaggcg
     2161 gcgcggcgct catgatcgtc gggtgcgtcg cggagcaaca gatcgctgcg cggcgtcagc
     2221 ccggcgagga tcgcttcggt cggaaactcg tcgcagcgga aatcccaata gcgttgggca
     2281 agcgcgtcga gggcaaggct gggggacgtc atgctgttca ctccttgatg ccgtcggcga
     2341 cggcgcagcg gcgcggatcg gcgactgccg tgagggtgcc cgacgcttcg tcgcgcgcga
     2401 tcaccgagaa gctgcccatg tggaagtcgt agcccgacag cccgccgacg cggacgccga
     2461 gcttgtgcag gtcctggacg acgccgggtg cgagccgatc ctcgatcgcg acgagttgtc
     2521 catcggtcat cggcagcatg cgcggcgcgt cgaccgcggc atagggatcg agcttgaagt
     2581 cgagcagata ggcgagcacc tgcggcagcg tgcagtggat gttgcccggc gaccccgccg
     2641 agaagaccgg cttgccgtcc ttgagcacca gcgtgttgcc gatgatgcag cgcgctttcg
     2701 cgcccttgat caacaccgta tccattgggc tttgcagcgc gccgaaggtc gagtggctgc
     2761 cgaccatcgg aatgccgccg acgacctgcc ccggaatgcc cgagccctgc agcgtgtcca
     2821 tgaactggca ccaattgcct tcggcatcga ccaccgcgat ctcgcagctg ccgctcggct
     2881 gcttttcctc atgatgcacg agccgcggct tgcccgaggg gcccgcgatc gccgccatca
     2941 tgtcgcccgc gccgccgggg ccgtcgcccg acaggcggat atgatcggtc atgtcgacct
     3001 tcggccgcga cccgcggatc agcttggcga gatgcttgtg ataatcgtcg tcgagcactt
     3061 cggcgcgcgg cacgtcatag acatgatcgt cgcgcgcata ttcccaatgc cgctggcctt
     3121 ggcggagcgc atgacccatt gcccagatat gctcggccga gcccggcgcc atgtcgcgga
     3181 tgccgagatg cttgagcaca ccgagcgcga cgcttgagaa gaagccctgc gcctgtgggg
     3241 gcccgagaaa gaggatttca tattcattat aggcgaagcg cagcggctcg gcccagcgcg
     3301 gcggggtctc ggtcatatgc tcgagcttga ccttccagcc gaggtcattg cccttggcga
     3361 cgaatttctc ggcccacgcg ccggtgatca tatggtcggg gccgttgtcg cgcacgccgc
     3421 gtagcgtttc tgccatgccc ggcggcgtga agacgtcgcc gacgttgggg aaatagccgt
     3481 tcggctgata gaaatcgcgg ccctcgggga aataggtgat gaacttctcg ccatagatgt
     3541 tcacgccata ttcgaaggtc gacacctcgt gcccggtctc ggcccagcgc accgcgtcgg
     3601 cgcagagatc ggcccagtcc ttggtcgcga atttggcgtg gatttccttg agccccggca
     3661 tgaagcccgg gatgatcgcc gacggcggcg aagtcgcata gctgccgagc cccggcggca
     3721 ccggcttgaa cggcggcagg cccgacggat gcccgcccag gctgtcgagc tggtggatct
     3781 tgcccgtctt cgcctcataa tagagcatgg tgacgagccc ggcgtggttg gtcatgaaag
     3841 gttcgaccgc cgcctgcacc atcgcccccg cgatcaccgc gtcggcggcg ttgccgccgc
     3901 tgttcaacac cttcatgatc gtctcggtga cgatcgcgct gtcggtcgtt acggccgcct
     3961 tcaatccggt taccggcgtc ttgggccccg ggttcacgat atagtttgaa aggtcggcca
     4021 taagtctctc ctttgattat gattccggtt cccgtgtcag agcacgacgg tcttgttccc
     4081 gttctggaaa atgcggtgtt cggcgtacca gcgcaccgcg cgcgacagca ccgacgcctc
     4141 gatatcgcgc ccgatcgcga ccatttcgtc ggcggtcgtc cggtgactga cgcggcggac
     4201 gtcctgttcg atgatcggtc cttcatcgag atcggcggtg acgaaatgcg ctgtcgcgcc
     4261 gatgatcttg acgccgcgcc cgtgcgcctg atgatagggt ttggcgccct tgaagctcgg
     4321 caggaagctg tggtggatgt tgatgcagcg ccccgcgagc gcctccgaaa aaccgtcgga
     4381 caggatctgc atataacgcg caagcacggt gagttcggcg ccgctctcct cgatgatcga
     4441 caggaaccgc gcctcctgcg tctccttccc ctcgctcatc ggcaggtgat gataggcaag
     4501 gccgtgccac tctgcggtct tgcgcatcgt ctcatggttc gaaaccacgc cgacgatatc
     4561 gatcggtagc tggccgatct ccttcttgtg gatgatgtcg atcaggcaat gcccgaattt
     4621 cgacaccgcg agcagcacag gcgtacgccg ggcgaggtcg tgcaattccc attcgagcgc
     4681 cagctcggtc gcgaccggct cgacgacctt cgccagttcg tcgcgcccga ccgggctcgt
     4741 cagcgccatg cgcaggaaaa agccgcaggt gtcgtcgtcg ccataaacct cggcctcgcg
     4801 aatgtcgcag ccgcgcgcgg cgagcgtcgg cgcgaccgtc gccatgatcc ccggcttgtc
     4861 gggcgcgcgg aatttcagaa cgagattcga ggtcgtcaca aaacagctcc tgcgatggtt
     4921 gcgccgagca tccgctcggc atagcccgcc atcgtctgat cgaagatcag ccggaagcac
     4981 ggctggccgt cgcgatcgga ccggcggctg atgaagaatc cggtgtccga gaacatcgat
     5041 cgcttcgcag caccgaccgg catcgcttcg tcgcccagat cgagcggaca atgcgtcgcg
     5101 agcaccgagc gcgcgtcggc gccacttaat tcgaatgtcg cgcggccatg ggtgacatcg
     5161 accatcaggc caagattgcc cgcggcttcg gcgcagcgca gccggacgcg ctcgacgtcg
     5221 gccgcatcgc cggtaacgag ccactcgccc ggcgccagcc aggcgatggc tataggaccg
     5281 ggctcgatct cggttagcgc cggaggcggc gcaaggccaa gcgcctgttc gaaccaggca
     5341 tcgggatccg ccgcgaggat ctgcagtttc gcaatgccga gctgggtttc cgccgcgaac
     5401 cgaacacccg ctccttcgac caacaaatct ttagcggcgg ccggcgctag cgacatggtt
     5461 cctacatcag ccatcgagtt tgcctcccgc aggatcgtaa aagcgtggat cgacgatccg
     5521 cgccgggcgc cctttgccga ggttccagac ccgcaccgtc tcgccaatcc gcgccgtacc
     5581 gcgttcgatc agcgccatcg cgagcggacg gccgagcgtc ggcgactgga ccgaagaggt
     5641 gacccagccc tgcgtcgcac cgatgccttt cgcatccgcc gccacgatat gcgcgccgca
     5701 ttcgaaggcg ccgccggcgt cgagcgtctc aagacccacg agttgacggc gatcgccgcg
     5761 cagtccttcg ggggtcatcg tcgagcggcg gccgatgaag tcgtcgggct tcttcgaaac
     5821 cgcgacgccg aaacccacgt cctgcggcat cgtcgtgccg tcggtgtccg acccgacgtg
     5881 cagaaacccc ttttcaatgc gcagcgccat cagcgcctcg acgccgaagg gcgtgatccc
     5941 gtgcggcgcg ccggcctgca tcatcgcgtc ccacagcgcg cggccatagc cccacggcac
     6001 cgaaacctca taggacagct cccccgagaa gctgacgcgc tggatgcgcg cgggaactcc
     6061 gccgacccgc ccttcgcgat agctcatgtg cggaaaagcc tcgcgcgaca ggtcgatgtc
     6121 ggtaccgatg ccctccagca ccgcgcgcgc cttcggcccc gcgacattca tcaccgccca
     6181 agcggtcgtc acattttcga cgaggacctt gaggtcggtc cattcgcact gcagccattc
     6241 ctgaagcatg tcggcgatcg cggcggcgtg gccgctcgtc gtgccgacga ggaaatgatc
     6301 ctcggcgatc ttcgcaaaaa cgccatcatc atagacgatg ccatgctcgc tgagcatcag
     6361 gccgtaacgg cagcgcccga ccttcaaatt gcggacgccg ttcacataga tgcgctggag
     6421 gaattcggcc gcatcgggcc ccttgacctc gatcttgccg agcggcgagg cgtcgaacag
     6481 accgacctgc ccgcgcaccg cgagcacttc gcgcgcaacc gcggcatgct cgtcttcgcc
     6541 ggtacgggga aaataggcgg gccgcagcca cgagccgtaa ttctccatct tcgcgcccgc
     6601 cgcgagcgcc gacgcgtgcg cggccgaatg cgcgatcggc atcagattct cgccgaccgc
     6661 acgccccgcg aaaccgccga tcgtcaccgg atcgaagggc gggcggaact tggtcgtgcc
     6721 gatcgcctgc ggcggcttgc cgagcaggct cgccagcatg tggatgccgg tgacgttcga
     6781 ggtcttgccc tggtcggacg ccatgccgag cgtcgtgtag cgcttgagat gttcgaccga
     6841 gcggaaattc tcgcgcgccg cgatctgcac gtcgcccgcg gtcacatcgt tctggaaatc
     6901 gacccacgct tttgccgcgc gcatatcgcc gtcggcgaag tgcagcgtcg gcccgaccgc
     6961 cgcggggaat ggcgccccga cctcgtcgcc ctgccccgcc gcccggccgc tggcgagtgc
     7021 gatttccaga tcgaatatgc ccgccgccgc gccgacattg accgcggcct gcaccgactt
     7081 gctcggcagg aaggcctcga gcgatgaatt gaacgcgagc gagccgcccg actgcgaatg
     7141 gaggtggacg gccggattcc agccgttcga catcaatatg gtatcgcagg cgatcgtttc
     7201 gctcgccttc ccgtcggcga tttttccaag ggtcaggcct ttgacgcggc gacgcccctt
     7261 cgccttgatc ggcgcactat ccatgatcgt gcgaatgccg cgcgccatcg cacccgcgac
     7321 cgcgttgccg cccttcgtcc ggctgtcgac gatcgccgcg atcacgaccc ccgcatcgtg
     7381 gagcgcgaag gccgcatgat aggcgctgtc gttgttggtc gcgacgacga tgcgccgccc
     7441 tggcgcgacg ccatagcgca acgcataggt ctgcgccgcc gacgccagca tgacgccggg
     7501 cagatcgttg ccggcgaagg cgattggccg ctcgaacgcc ccggtcgcca ggatcacgcg
     7561 cttcgtgcgg actttccaca accgctggcg cggcccgctg cgcgcttgcg gcggcagatg
     7621 gtcggtcacg cgctcgcaca agccgacaag ttgatggtcg tagaagccga acgccatcgt
     7681 ccggttcatc accgtcacgt tcggcaggcc cgcaagcgcg accagcatcc ggtcgcgcca
     7741 tgcaagcggt gccaccccct cgatctcgga cggttcggca agcagcccgc cgccgagttc
     7801 ggcgtcggcc tcgacgagca gcacctgttc gccacctttc gccgccgcga tggcagcggc
     7861 gagacccgcc gcgcccgatc cgaccaccac gatgtcggtg tgcgcgaagc gatgatcgta
     7921 gctgtcggga tcggactctt ccggcgcatc gccgagtccc gccgccttgc ggatcgcggg
     7981 ttcgaaccag tgccagttcg gccacatgaa ggtcttgtaa tagaaggcgg cgggaatgaa
     8041 gggtttgaac aggctattca ccgcgagcag gtcgaactcg acgctcggct tggcattgac
     8101 cgggttcgcc accagccctt cgtaaagctc gatctgcgtc gccttgacgt tcggcaccgc
     8161 cgctgcgccc gtttccagct ggacgatcgc gttcggttct tcgagccccg ccgcaagcag
     8221 cccgcgcggg cggtgatatt tgaagctgcg cccgacgatc gacacgccat tcgccatcag
     8281 cgccgacgcg agcgtatccc cggcatagcc gtggagcgtc cgccccgccc agcggaatgc
     8341 gacctgcttg ccgcggtcga cacggcccgc cgtgcccaga cgccagccgc tcatacgatg
     8401 cctccttggg ccgcgaccgg tgccggctcg ctcaccgcgc tctcgcccgt ttcgggacag
     8461 gcgtcgccca tcttgtaggt gacgcggaac cggtgcgtca ccgtgtcgcg cgcggcgttg
     8521 aaccagcggc ggcatccggc ggcatggaac cagcgctcgc gatgctcgcc tttcggattc
     8581 tcgcgataga agagataatc ggcccattcg gcgtccgaaa cgcggtcatg cggtcccggc
     8641 cgctggatat ggctttcgcc gccgcagcgg aattcgatct cggcgcgcgg gccgcaaaag
     8701 ggacagtcga tcagcaacat tccttgaata tccccctagt gcgcgatgcc cgaggccgag
     8761 gcctcgtcga tcatcgcgcc cgtctcgaag cgttcgaggc cgaaagcgct cgccagcggg
     8821 tgcgatttgc cggtggccat gacatgcgcg agacaccagc cgcccgccgg caccgccttg
     8881 aatccgccgg taccccaacc cccgttgatg tagagtccgc cgatcgacgt cgggccgatg
     8941 atcggggtcg agtcatggac gacatcgacg atccccgccc attgacgcag aaaccgcatg
     9001 cgcgagaatt gcgggaagag ttcgagcacg cccgcagcgg cgcgttccat ccacggcaaa
     9061 ttgccccgct gcgcatagga gagatagagg tcgagccccg cgcccagaac gagctcgccc
     9121 ttgtccgact ggctgagata ggtgcccgtc gcgggtgaga ggatcaccgt gtcgaggcac
     9181 ggcttcactg gctccgacac gaaagcctga agcgcgtagg aactgatcgg tagccgcagc
     9241 cccgcctttg ccgcgagcac cgaactatgc cccgcgaccg ccataccgat ccggtccgag
     9301 cggatcgtgc cgcggctggt ttggatccct cgcacctgcc cgccttcgat gatgaagtcc
     9361 tggacctcgc aattctgtat gatgtcgacg ccgagcgccg acgccgcgcg ggcgtagccc
     9421 cacgcgaccg cgtcgtgccg cgccgacccg ccgcgcggct ggttgacgcc gccgaggatc
     9481 ggaaagcgcg ggttcgggtt gaggccaggg atattggcgt agacgctcgg cccgtcgtga
     9541 tattcggcat cgatgccgtt gagcagcatc gcgttcgccg agcgccgcgc catttcgaca
     9601 ccgtgccggt cgtgcgccag cgtccacatg ccgcgctgcg agaacatgat gttgtaattc
     9661 agctcgtcgg tcagcttctc gtagagctgc accgaaaatt cgtaaaaggc ggccgattcc
     9721 ggaaagaaat agttcgagcg gatgatcgtc gtgttgcggc cggtgttgcc gccgccgatc
     9781 cagccgcgct cgatcagcgc gacattggtg agaccgtgat tcttggcgag gtaataagcg
     9841 gtcgcaagcc cgtgcccacc gccaccgatg acgatcgcat cataatgcgt cttcggcgtc
     9901 ggatcacgcc acgccggctc ccagccgcgc tgaccgttca atccttcgcg caaaagcgac
     9961 agcgccgaat agcgtctcgt caagactcac cttcctctcg ccttttagat gcacatatgt
    10021 atgcaaatgc gcaacaggaa ttttcggaag aatttcagac aggcctcttc gagccaacga
    10081 caagaaatgg agccctaatt tcacgatctt ggccatatgc cggcacatag gagaaacggt
    10141 ccaacccgcg ctctcactcc tcacagaagt gcatttccga cgaaatccat tctttcagat
    10201 tccgcacata gggtcccatg gcgcgcggca atcaggccga cgtctcggaa atgcgcgcct
    10261 agcggaaaat cgacatatta tactgccgaa agagcgatca gcacccaaat cgccggtcag
    10321 cgaaacatcg cctcgcccga tcgcgcgttc attttggatc tttagggcat acttttattt
    10381 catgcagaaa tgcatatatg tccgcggcta ggccaacccg tcgaatcggc aagggagata
    10441 cggatgacga tcgcgcgttt aatcgacggc aaacagcggg cggccgcatt gcgtcgcgat
    10501 atcgcaatcg gcgtcgcgca ttttcgtgac gaaacgggaa ctccaccaaa gcttgccgcc
    10561 attctcgtgg gtgacgatcc cgccagcgcg atctatgtgc gccgcaaagg cgagcaggcg
    10621 gcggccgtcg gcatggaaag cgaaagccat cggctcgacg ccgatatcgc cgaagccggg
    10681 ctgctgtcgc tgatcgatac gctcaaccgc gacgaaagcg tgcatggcat attggtgcag
    10741 ctccccctcc cgccccatat cgacaaggcg gcggtgctgc gcgccatcga tcccgcaaag
    10801 gatgtcgacg gctttcaccc aatgaacgtc ggcctgctcg cgagcgatgc cgaggccgcg
    10861 gcggagcgga tcgttccgtg cacgccgatg ggctgcatga tgttgctgat cgacgaactc
    10921 ggatcgctcg aggggttgaa tgccgtggtc gtcggatgct cgaacattgt cggccggccg
    10981 atggcgcagc tccttctgca agcgcgctgc accgtttcga tcgcgcatat ccatacgcgg
    11041 gatctgcccg aactggtcgc cggcgcggac atcctggtca gtgccgccgg cgcaccgggt
    11101 ctgatcaagg gagactgggt caaacccggc gcggtcgtga tcgacgttgg catcaaccgc
    11161 gtgcccgatg ccgatggccg gacatcgcgg atcgtcggcg acgtctgctt tgacgaagcc
    11221 agaaagcgcg cgtcggccat cacaccggtt ccaggcggcg tcggcccgat gaccatcgcc
    11281 tgtctgctcc ggaacacgct gtccgccgcc atcgcgcttg aagcgcaggg agtcgcgaat
    11341 gccgacgcaa gcgccatgcc gcgatccgcc gggcgccacc tcggcggaaa gttacaatgc
    11401 atcaatgaat gaaaatagag ctgccacgcc gctcggcgcg gcgcggtcgc gacgagaacg
    11461 gaaacgagtc ggaatttggt cgtgataccg agccgaatcg cggtgaattt ctgattatgc
    11521 caaggcggcc ctgccgagcc taccccgcca cgcaggagtg cggcgcctca tcttcgtcca
    11581 tttaatatac tgtaaaacga tgatttttat caaaactagg gaaggtgacg atctccttgc
    11641 taattgaata catttatgta tcaattagcc catcggtcga aagatgatcg gaaagcgaag
    11701 cgtcacgggc ggcttcgggg aggaacgtcg gatgaagaca ggctctcgaa caattcgatc
    11761 cgcccgcccc gcggccgatg tcgccgtcgc tcgactttcc gcgctttcgg gaccggtgaa
    11821 aaaggggaga gatcatgcag cataggaatt tgtggttcgc gaccgcggcc atcggcgcac
    11881 ttctgaccgc caccggcgcc caggcgcagt cggccggtga ggatccggca tcgacagccg
    11941 acggccagta tcacggcgcc gaaatcgtcg tcaccgccag caagcgtgaa gagcggctgc
    12001 gcgatgtccc taccgcgatc accgcgatcg gcagcgacgc gatcgagaca ctcggcatcc
    12061 gcgacttccg cgactatgcg acgctgaccc cggggctcag ccagcgcgat ttcggcgcgc
    12121 ccggcaccgg aacggtgatc ctgcgcgggc tcaacacggg gccgcagcag acgacgaaca
    12181 cctcggcctt ctacatcgac gacgcgccct attcgtcgaa cggttttctg tcggtgggat
    12241 cgctcgtcac gccgtcgccc gacctggtcg atgtcgagcg gatcgaggtg ctgaagggac
    12301 cgcaggggac cctctatggc gcgagcaacc tcggcggcct cgtccgcgtc attaccaaga
    12361 aggcggacac tgcggccttt tccggcatgg cgcaggtcga gggcgaaacg ctggcgcatg
    12421 gcaacgaagg ctgggcggtg cgcggcgcgg tgaaccttcc cgtcgttacc gacaagctgg
    12481 cgatccgtgt ttcgggtttc taccggaaga ccgccggctt caccgacaat gtcgaaacgg
    12541 gcgagaacaa cgtcaacagc tcgacgatca agggcggccg tatcgcgctg cgcgcgacgc
    12601 caaccgagcg actgacgatc gacgtcaccg gcttttatca ggacatcgaa aacgacggca
    12661 gctcgggaca ggacaatgtc accggcacga cccgcccgct gttcggcaag tacaagttca
    12721 gcgcggtcga ggggaccggc ggcagcaaga tccaatatcg ctcggtgggc ggcaccgcga
    12781 gctatgagac cgatgccggg gcaatcgtgg cggtcgcgaa ttatgtgaag acgcagtcgg
    12841 cgatcagctc ggactatacc gacgtctatg gcagctatct ttcgctgctc ggtatcgttc
    12901 cgccgggcac gcagacgatc ggcgatcttg gccccaatat gaagaaattc tcggccgagc
    12961 tgcgcttcgt gtcggagcgg atcggggcgt tcgagttcgt cgcgggcggc ttctatacca
    13021 acgagaaaag ccgctatcct ttcatcatca ccgcgcgcga cgcgacaggc acgcccctgc
    13081 ccgcgccctt cgacatgctg ttccgcagca cctccttcgc gaattacgag gaatatgcgg
    13141 cgttcggcaa cctgaccttc tatctcaccg accggctcga tgtgaccggc ggcatccgct
    13201 atgcaaagaa cgaccagtcg tcgagcggcc ccggaggcat cctcttctat gcgccgacgc
    13261 cgccgacctc gacctcgttc agcgacgatg cgacgaccta tctcgcgacg atccgctatc
    13321 gcccgagcga caatgtcagc ctctatgctc gcgccgcgag cggctaccgc cccggcggcc
    13381 cgcaaaattc gacgctgctg ccgccgacgg cgcagacggt gatccgcgcc gatacggtgt
    13441 ggaattacga agccgggatc aaggcgaccg cgctcgacgg cgccttgtcc gccaaccttg
    13501 ccgtctatca tatcgactgg aaggatattc agctcaacac gctgttcggc ggcgtgacgc
    13561 tgcagggcaa tgcggggtcg gcgaaggtcg acggcttcga actcgagctc caggcgcgac
    13621 cgtcgcggaa tttcgtcttc ggcgccaatg tcggtcacac caatgcccgg atcagcaaga
    13681 tcgatccggt cgccgcggca tcgctcggcg cgcagtcggg cgacaagctg ccgctcaccc
    13741 ccagctggac cgtcgcggcg ttcgccgatc agaaaatccc cttctccgac agcgtgaagg
    13801 gctcgatcgg cgcgaccatc cggctccagt cggacatgcc gagcagctat ccgggctatg
    13861 cgctcaatcc caacatcaag atcccgtcga tcacgacggt cgaccttcgc gccgggctcg
    13921 aaatgtcgcc cgtgtcgctc cagctgcggg tcgaaaattt gttcgacaag ctcggttaca
    13981 cgtcgctcgc gaccaacgcg ctctacgtcg gtcagccggt gccgaccaac gcgacggtga
    14041 cgcgcccgcg cgccttcatc ctgtcggcca ccgccaactt ctgattgagg ggatattagg
    14101 gatgacccgg aaaatcgatc gccgcgccgc gctgctcctc gccggcggac tcgccgccac
    14161 caccgcggcg ccggccatgg ccaagacacc caaggccgcg cccggcacgt ttcagccgat
    14221 gccgatgccc tatgcgtcga tgttcccgcg cacgaaatat ttcgagatcg attcggctcg
    14281 cgccggcgcg cgctatgcgg tctgggtctc ggttcccgag gtctatgaca aggatccgtc
    14341 gcgcagtttc cctgcggtat tcgcgcccga cggcaatcat ttcgtcgcct tctcgacgac
    14401 gctctgcgaa ctcggccagt gggatctgat cgacaaatat cagtccacga tccaggtctg
    14461 catcggctat accggcaagg atgccgacgt ctcgctcgcg gtacgcgcgc gagatctgtt
    14521 gccgccgaag gagccgctgc cgccgggcat ggtcgatgcg atgcggaacg gcggcgcgtc
    14581 ggacctgctc gataaggcgg gaatcgagct ttacatccac aatctggaaa atccggcggc
    14641 ggaccgcttt ctcgctttcc tgagcgaaga actctacccg ttcatcgcga aaaactatcg
    14701 catccagtcg gacaatctgg ggcttttcgg ccattcctac ggcggccttt tcaccaccta
    14761 tgccgcgctt cagccaaaga cgatcttccg gaatttcggc gcgagcagcc ccggcatcct
    14821 gcccggcgca tcgaccgtgt tcaaactcca tgccgatgcc gcggcagccg gagggctcag
    14881 cgagcgcaac ctgcatctga ccgtcggcac gcgcgaactg acggtgccgg ggctctacca
    14941 atatatggtc ggtgccgggt cgaccgaatt catccggact gcgggcacaa cgccggtcaa
    15001 ggggctgagc ttcacaagca agttgatcga ggacgagagc catatgacat tggcccaccc
    15061 cgccgcgttc gatttcatgc ggcaatatta tcgccggcag gcataagatc aggccgcggc
    15121 gcggctggcg gcttcgcgcg ccgccagctc cgcgctgac
//
LOCUS       DATMSU010000503         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_160190,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000503 DATMSU010000000
VERSION     DATMSU010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160190"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="VN034_13335"
     CDS             <1..946
                     /locus_tag="VN034_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HWV61639.1"
                     /translation="PMISGLPPVQVTASGETQPVGTNRADAADDPAIWVDPANPNRAL
                     IIATDKKAGLHVYDLAGKDLAFIEGGLVNNVDVAGDIIVASDRNDGVNAHLAVFRLDA
                     GKPAITALGRAAAGTGEAYGLCLKKGLPGQPITAALIVKDGTVRVGTLTIEGTPSFAV
                     QWEHKIPTQSEGCVFDGDTLYVGEEVGGIWELKPNGNSATARLVAPVDNQRLVADVEG
                     LATIDHKGQRYLIASSQGDNAYAVFRLPGMDYVGRFAVAAGAFGATSETDGIEAVAGN
                     FGPDYPDGLFLAQDGDNGALAQNFKLVRWDRIAAALGF"
     gene            1059..>1910
                     /locus_tag="VN034_13340"
     CDS             1059..>1910
                     /locus_tag="VN034_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61640.1"
                     /translation="MAIIRYAGVAVSTLALAGCAFGPQMQRVAIDQNAMIADTANELM
                     VVNILRARNREPLHFTSIAKATGSATLSGTVSGDVAIQGATAAETTGSTGSLTSTTTT
                     PGVEVFTPALEASIVTGSNLDIAVFDTQEFYQGITTSLTSSQVAHYLHQGWPPELLMY
                     LMIESVEFVPAPEDGSPVIDVAWKNDPDDDDAMKRFADFVARYPIDLGVKPEKIDYVA
                     PVSEFSGSKLADFLQLDGKRFTIADREIGENKTERWLVRLSGGGDDVVVKSLDKAADG
                     VAGCPAAP"
BASE COUNT          346 a          588 c          658 g          318 t
ORIGIN      
        1 gccgatgatc agcggcctgc cgccggttca ggtgaccgcg agcggcgaga cgcagccggt
       61 cggaacgaac cgcgccgacg ccgccgacga tcccgcgatc tgggtcgatc ccgccaatcc
      121 gaaccgcgcg ctgatcatcg cgaccgacaa gaaggcgggg ctgcacgtct acgacctggc
      181 gggcaaggac cttgccttca tcgagggtgg gctggtcaac aatgtcgatg tcgcgggcga
      241 catcatcgtc gcgagcgacc gcaacgacgg agtgaacgcg catctcgcgg tgttccgcct
      301 cgacgcgggc aagcctgcga tcaccgcgct cggccgcgcc gcggcgggga cgggcgaggc
      361 ctatggcctc tgcctcaaga agggcctgcc cggccagccg atcaccgcgg cgctgatcgt
      421 caaggacgga acggtgcgcg tcggcacgct gacaatcgag ggcacgccga gctttgcggt
      481 gcagtgggag cacaagattc cgacccagtc cgaaggctgc gtcttcgacg gcgatacgct
      541 ctatgtcggc gaggaagtcg gcggcatctg ggaattgaag ccaaacggca atagcgcgac
      601 cgcgcgtttg gtcgcgccgg tcgacaacca gcgcctcgtc gccgacgtcg agggcctcgc
      661 gacgatcgat cacaagggcc agcgttacct gatcgcgtcg agccagggcg ataacgccta
      721 tgcggtcttc cgcctgcccg gcatggacta tgtcgggcgt ttcgcggtcg cggcgggcgc
      781 attcggcgcg acgagcgaga ccgacgggat cgaggcggtc gcgggtaatt tcggccccga
      841 ctatcccgat ggcctcttcc tcgcgcagga cggcgacaac ggcgcgctcg cgcagaattt
      901 caagctggtg cgctgggacc ggatcgcggc ggcgctgggg ttttgatgcg gcgcgccggt
      961 tgccgggaaa ccggcgccga attggttcta tttgcgcgtt gatcgcgccg ctgctccggc
     1021 taaactccct tccatcataa cggatggggg gattcggtat ggcaatcatc agatatgccg
     1081 gtgttgcggt gagcacgctt gcgctcgccg ggtgcgcttt cgggccgcag atgcagcgcg
     1141 ttgcgatcga ccagaatgcg atgatcgccg atacggcgaa cgaattgatg gtcgtgaaca
     1201 tattgcgcgc gcgcaatcgc gagccgctgc atttcacctc gatcgccaaa gcgacgggca
     1261 gcgcgacgct gagcggaacg gtgagcggcg atgtcgcgat ccagggcgct acggcggcgg
     1321 agaccacggg ctcgacggga agcctgacga gcacgaccac gacgcccggg gtcgaggttt
     1381 tcacgccggc gctcgaagcc tcgatcgtca cgggatcgaa cctcgacatc gcggttttcg
     1441 atacgcagga attttatcag gggatcacga cctcgctgac ctcgtcgcag gtggcgcatt
     1501 atcttcacca aggctggccg cccgaactgc tgatgtatct gatgatcgaa tcggtcgaat
     1561 tcgtgcccgc gccggaggac ggatcgccgg tgatcgacgt cgcgtggaag aatgatcccg
     1621 acgacgatga tgcgatgaag cgtttcgccg attttgtcgc gcgctatccg atcgacctgg
     1681 gggtaaagcc cgaaaagatc gactatgtcg cgccggtcag cgaattttcg ggatcgaagc
     1741 tcgccgattt cctgcaactc gacggcaagc gtttcacgat cgccgaccgc gagatcggcg
     1801 agaataagac cgaacgctgg ctggtcagac tgtcgggcgg cggcgacgac gtcgtggtga
     1861 agtcgctgga caaggcggcc gacggcgtgg ccggatgccc cgcagcgccg
//
LOCUS       DATMSU010000504         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_122409,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000504 DATMSU010000000
VERSION     DATMSU010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122409"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VN034_13345"
     CDS             complement(<1..470)
                     /locus_tag="VN034_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HWV61641.1"
                     /translation="MFSSLRKLFKSEPDPREARRPLWDAVVATARAPHWYAQGGVPDT
                     LDGRFDMISLVLALVLHRIDEDPEQGLAGVQLTELFVNDMDGQMRQIGFGDMVVGKQV
                     GRMMSALGGRLGAYRAADGSAELREALVRNLWRGNAPSEAGLAHVMTEVAALRAA"
     gene            645..1130
                     /gene="bamE"
                     /locus_tag="VN034_13350"
     CDS             645..1130
                     /gene="bamE"
                     /locus_tag="VN034_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HWV61642.1"
                     /translation="MPHSILSLPAPRARLVVLGLAVALATSGCAQLKGRQGYVADPAL
                     TQAITPGVDNRESVEKTLGRPTFVGQFGTDEYYYVSRETRQLAFARPRPVDQQVLRVR
                     FDEAGNVAAVDRTGLELVRKISPEGDKTPTLGRERSFFEDIFGNIGAVGAPGAGAPGG
                     R"
     gene            complement(1346..3610)
                     /locus_tag="VN034_13355"
     CDS             complement(1346..3610)
                     /locus_tag="VN034_13355"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HWV61643.1"
                     /translation="MDSGSKVQFSDREALLYHEYGRPGKIEIVASKPMATQRDLSLAY
                     SPGVAVPVNAIAEDPAKAYDYTIKGNLVAVISNGTAILGLGNLGALASKPVMEGKAVL
                     FKRFADVDSIDLEVDTEDPQRFIEAVELLAPSFGGINLEDIAAPNCFIIEAALKERMN
                     IPVFHDDQHGTAIITAAGLINACYLTGRDLATVKVVVNGAGAAAIACTELIKAMGVKH
                     ENVIMCDRKGTIYQGRTDGMDQWKSAHAVPTEARNLTEALVGADVFLGLSAAGALKPE
                     MVKDMSPAPIIFAMANPDPEISPPDARAARPDAIIATGRSDYPNQVNNVLCFPFIFRG
                     ALDVHATAINEEMKIAAAYAIADLARQQVPEEVAAAYGGRASSFGPEYIIPSPFDPRL
                     MEIVPAAVAEAAMKTGVAQRPIEDLGEYRTRLRARLNPTTSVLTLAYEAARNNPKRVV
                     FAEGEEEVVLRAAIQFRDGGYGIPVLVGREGLHDKLRAMGVADAESFEVHNSVNSPHV
                     PQMVDMLYERLQRRGYLRRDVERMVNRDRNIFGSLLLKMGLGDAMITGTTRTYSQSMR
                     EIRRVIDHAEGKTPFGIHVLVDQHHTIFMADTTVNERPTAEMLADIAERTAQVARRMG
                     HEPRVAFLSYSTFGNPEGSWLDSIREAVSILDQRQPGFEYEGEMAPDVALNEKVMKNY
                     PFCRLSGPANVLVMPGLQSANLSAKLLRELGGGAVIGPMLVGMEHPVQVATMASTASD
                     LVTLAVLAAGGIAQ"
BASE COUNT          632 a         1228 c         1269 g          686 t
ORIGIN      
        1 gccgcgcgca gcgccgctac ttcggtcatc acatgcgcca gccccgcttc ggagggtgcg
       61 ttgccgcgcc acagattgcg gaccagcgcc tcgcgcaatt cggccgagcc gtcggccgcg
      121 cgataggcgc caagccgccc gccgagcgcg ctcatcatcc gcccgacctg tttgccgacg
      181 accatgtcgc cgaaaccgat ctgacgcatc tgcccgtcca tgtcgttcac gaacaattcg
      241 gtgagctgga cgccggcaag cccctgttcg ggatcttcgt cgatccggtg cagcacgagc
      301 gcgagcacga ggctgatcat gtcgaagcgg ccgtcgagcg tgtcgggcac acccccttgc
      361 gcataccagt gcggcgcgcg cgcggtggcg acgacggcat cccacagcgg acggcgcgcc
      421 tcgcgcggat cgggttcgga tttgaagagt ttgcggagcg aagaaaacat ggcgattcgc
      481 ttaggctcat tcgacccgcc cgaaaagggt cctcggtcag cctgtggcaa gctttgccgc
      541 ggcacaagcg gcttgtgcgc gctcaccgct tgcggttaag aggcgcgaga gagccagcgc
      601 cgaccgccgg gacaagcggc ggacaggcgc aacggagata tttgatgccg cattcgatcc
      661 tttcgcttcc cgccccccgc gcgcgcctcg tcgtgctcgg ccttgccgtc gcgctcgcca
      721 ccagcggctg cgcgcagctc aaggggcggc agggctatgt tgccgatccg gcgctgacgc
      781 aggcgatcac ccccggtgtc gacaaccgcg agtcggtcga aaagacgctc ggccgtccga
      841 ccttcgtcgg tcagttcgga acggatgaat attattatgt ctcgcgcgaa acgcgccagc
      901 tcgccttcgc gaggccgcgc ccggtcgatc aacaggtgct gcgcgtacgc ttcgacgagg
      961 cgggtaatgt cgctgcggtc gatcgcaccg ggctcgagtt ggtccgcaag atcagcccag
     1021 aaggcgacaa gacgccgacg cttggccgcg aacgcagctt cttcgaagat atcttcggta
     1081 atatcggcgc ggtcggtgcg cccggtgcgg gcgcgcccgg cgggcggtaa tctccctata
     1141 tgatcctccc tgcggtgaag ccgtggggag gtggcagcgc ggagcgctga cggaggggca
     1201 ttggcacaac cgttgccgcc cctccaccac tcgcttcgcg agagtgctgg gtcgtccagt
     1261 cccccggact ggacttggat tgccgggggc aatccaaccc tgcactcccc ctccccatgc
     1321 ctgcggcaca gggagatttt cagtcctatt gcgcgatccc gcccgccgcg agcacagcca
     1381 aggtaacgag gtccgacgcg gtcgaggcca tcgtcgcgac ctgcaccgga tgttccatgc
     1441 cgacgagcat cgggccgatg acggcgccgc cgccgagttc gcgcagcagc ttcgccgaca
     1501 ggttcgccga ttgcagcccc ggcatgacca gaacattcgc gggacccgac aggcggcaga
     1561 agggataatt cttcatcacc ttctcgttca atgcgacgtc gggcgccatt tcgccttcat
     1621 attcgaaacc gggctggcgc tggtcgagga tcgacaccgc ctcgcggatg ctgtcgagcc
     1681 agctcccttc aggattgccg aaggtcgaat aggagaggaa ggcgacacgc ggttcgtgcc
     1741 ccatgcgccg cgcgacctgc gcggtgcgtt cggcgatatc ggcgagcatc tccgccgtcg
     1801 gccgttcgtt gaccgtcgtg tcggccatga agatggtgtg atgctggtcg accaggacat
     1861 ggatgccgaa cggcgtcttg ccctcggcat ggtcgatcac gcgccggatt tcgcgcatcg
     1921 actgcgaata ggtgcgcgtc gtgccggtga tcatcgcatc gccgagcccc attttgagca
     1981 gcagcgagcc gaagatgttg cggtcgcggt tgaccatgcg ctcgacgtcg cggcggaggt
     2041 atccgcgccg ctgcagccgc tcgtagagca tgtcgaccat ctgcggcaca tgcggcgaat
     2101 tgacgctgtt gtggacctcg aagctttcgg cgtcggcgac gcccatcgcg cgcagcttgt
     2161 cgtgcagtcc ctcgcgcccg acgagcaccg ggatgccata gccgccgtcg cggaactgga
     2221 tcgcggcgcg cagcacgact tcctcttcgc cctcggcaaa gacgacgcgc ttcggattgt
     2281 tgcgtgcggc ctcataggcg agcgtgagga ccgaggtggt cgggttgagc cgcgcgcgca
     2341 gccgggtgcg atattcgccc agatcctcga tcgggcgctg cgccacgccg gttttcatcg
     2401 ccgcttcggc aaccgcggcg ggcacgattt ccatcaaacg cgggtcgaag ggcgagggga
     2461 tgatatattc ggggccgaag ctcgatgcgc gtccgccata cgccgccgcg acttcctcgg
     2521 gcacctgctg gcgcgcgagg tcggcgattg cataggcggc cgcgatcttc atctcctcgt
     2581 tgatcgcggt cgcatggacg tcgagcgcgc cgcggaagat gaaggggaaa cagaggacgt
     2641 tgttgacctg gttggggtaa tcggaacgcc cggtcgcgat gatcgcgtcg ggccgcgccg
     2701 cgcgcgcgtc gggcggcgag atttcaggat cggggttcgc catcgcgaag atgatcggcg
     2761 ccggcgacat gtccttgacc atctcgggct tgagcgcgcc cgcggccgac aggccgagga
     2821 agacatcagc gccgacgagc gcctcggtca ggttgcgcgc ttcggtcggc accgcatgcg
     2881 ccgatttcca ctggtccatg ccgtcggtgc ggccttgata gatcgtgccc ttgcggtcgc
     2941 acatgatcac attttcatgt ttgacgccca tcgccttgat cagttcggta caggcgatcg
     3001 cggcggcgcc cgcgccgttg acgacgacct tgacggtcgc gaggtcgcgc ccggtcaggt
     3061 agcaggcatt gatcaggccg gcggcggtga tgatcgcggt gccatgctgg tcgtcatgga
     3121 acaccggaat attcatgcgc tctttcagcg cggcttcgat gatgaagcaa ttgggcgcgg
     3181 cgatgtcttc gaggttgatg ccgccaaagc tcggcgcgag cagctcgacc gcttcgatga
     3241 aacgctgcgg atcttcggtg tcgacctcaa ggtcgatcga atcgacgtcg gcgaaacgct
     3301 tgaacagcac cgccttgcct tccatcaccg gcttcgatgc gagcgcgccg agattgccga
     3361 ggccgaggat cgcggtgccg ttcgagatca ccgcgaccag attgcccttg atcgtatagt
     3421 cataggcctt ggcgggatcc tcggcgatcg cgttgaccgg caccgcgaca cccggcgaat
     3481 aggcgaggct gaggtcgcgc tgcgtcgcca tgggcttcga cgcgacgatc tcgattttgc
     3541 cgggccggcc atattcgtgg taaagcaggg cctcgcggtc ggaaaattgc accttgctgc
     3601 cgctgtccat tctgtatcct ctcgaaattt ccacaagatg cgcgcccgaa tatttcccct
     3661 agcgccgccc cgtgaaaatg taaccggcaa tgttgcgcgc tggtgggcga ggggggggct
     3721 ttcgatcgga agcggacttc aaatcctccc tgtcgcgaag cggtgggagg tggcagcgcg
     3781 aagcgctgac ggaggggcaa tgacgcgacg tcccc
//
LOCUS       DATMSU010000505         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_83805, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000505 DATMSU010000000
VERSION     DATMSU010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83805"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VN034_13360"
     CDS             complement(<1..1113)
                     /locus_tag="VN034_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWV61644.1"
                     /translation="MARSAQKSLFTADLIVPAIGDAFRKLNPRELIRNPVMFTTAVVA
                     ALLTVLLVVGQDGLATGFKLQLVIWLWLTVLFGTFAEALAEGRGKAQAASLRATKAEL
                     TAKRLKGEGKAHDSVPASALKVGDIVLVETGDLIPSDGEVVAGVASVNEAAITGESAP
                     VIREAGGDRSAVTAGTRVISDEIRVRVTVGPGQGFLDRMIALVEGAERQKTPNEIALT
                     LLLVGLTIIFLIAVGTIPGFASYAGGSIPVAILAALLITLIPTTIAALLSAIGIAGMD
                     RLVRFNVLAKSGRAVEAAGDVDVLLLDKTGTITVGDRQATEFRAVGGHSAAELAEAAL
                     LASLADETPEGRSIVVLARESFGRTADLPAGAEIIAF"
     gene            complement(1126..2829)
                     /gene="kdpA"
                     /locus_tag="VN034_13365"
     CDS             complement(1126..2829)
                     /gene="kdpA"
                     /locus_tag="VN034_13365"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWV61645.1"
                     /translation="MTFQGWLLIAAFVGILLLLCKPMGQWLFALYEGRRTPLHRVLGP
                     VETGFYRLSGINPDEEQGWRRYAVHMLIFNTALLLFTYAVLRLQGVLPLNPLGYDGTS
                     ADLAFNTAISFTANTNWQSYGGESTMSNLSQMLGLTIHNFLSAATGIALAFALFRGFA
                     RRSATTIGNFWADMTRVTLYLLLPICVVIATFYIASGVPQTLAGSVDVTTLEGAKQTL
                     ALGPVASQEAIKMLGTNGGGFFNVNSAHPFENPTALTNLVQMLSIFLIGVGLTWTFGR
                     AVGNQRQGWAILAAMMTLFVAGAAITYWQEAAGNPILHNLGVPGGNMEGKEVRFGIAQ
                     SALFAVVTTAASCGAVNAMHDSFTALGGMIPLFNMQLGEVVIGGVGAGIYGFLLFAIL
                     AIFVAGLMVGRTPEYVGKKIEAREVKLAVLAIAVLPLIILGFTAIASVTEAGLAGPLN
                     KGPHGFSEILYAFTSAVANNGSAFAGLTANTPFYNGMLGVAMWIGRFFIIIPMLAIAG
                     GLAAKKYTPATAGSFPTTGLLWTGLLVGIVLIVGGLTFLPSLALGPIADHLAMINGQL
                     F"
     gene            complement(2842..2931)
                     /gene="kdpF"
                     /locus_tag="VN034_13370"
     CDS             complement(2842..2931)
                     /gene="kdpF"
                     /locus_tag="VN034_13370"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HWV61646.1"
                     /translation="MTLDLWLAGATALGLLVYLVAVLARPERF"
     gene            complement(3184..>3855)
                     /locus_tag="VN034_13375"
     CDS             complement(3184..>3855)
                     /locus_tag="VN034_13375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61647.1"
                     /translation="AVTPDAPAAGAPGLVAHIGGQRAALGALFGGMSLIGLVMYGAIA
                     WIPTFFARSYGMDVAQAGLWFGVIMAIGGALGLIAGGALADRLFAKGVGDAHLRVMRL
                     SILLGGPPLTAMALMPDPALAFAMLALAFPCLTMHGVGGVALQLITPNEYRARMTALY
                     FFVVNLVGLGFGPMLMALLTDNLFQDESALRYSIAIVTAAALPLAAIILTWGFKGYAR
                     ELARS"
BASE COUNT          754 a         1261 c         1267 g          573 t
ORIGIN      
        1 gaaggcgatg atctcggcgc cggcgggcag gtccgccgtc cggccgaagc tttcgcgcgc
       61 cagcacgacg atcgagcgcc cctcgggagt ttcatcggcg aggctcgcga gcagcgccgc
      121 ctcggcgagc tcagccgccg aatgaccgcc gacggcacgg aattcggtcg cctgccggtc
      181 gccgacggtg atcgtcccgg tcttgtcgag tagcagcaca tcgacatcac ccgccgcctc
      241 gaccgcgcgg cccgacttgg cgaggacgtt gaagcgcacc agccggtcca tgcccgcgat
      301 gccgatcgcg gagagcaacg ccgcgatcgt cgtcggaatg agcgtgatga gcagcgccgc
      361 gaggatcgcg accgggatcg aaccacccgc ataggaggca aagcccggga tcgtgccgac
      421 cgcgatcagg aagatgatgg tgagcccgac gagcagcaat gtcagcgcga tctcgttcgg
      481 cgtcttctgc cgctcggcgc cttcgacgag cgcgatcatg cggtcgagga aaccctgccc
      541 cggccccacg gtcacgcgca cgcggatttc gtccgagatg acgcgcgtgc ccgcggtaac
      601 cgcgctgcgg tcgccgcccg cctcgcggat caccggcgcg ctttcgcccg tgatcgcggc
      661 ttcgttgacc gacgcgacac ccgcgacgac ctcgccgtcc gaagggatca ggtcgccggt
      721 ttcgacgagc acgatgtcgc cgaccttgag cgcactcgcg ggcacgctgt catgcgcctt
      781 gccctcgcct ttcaggcgct tggcggtcag ctcggccttg gtcgcccgca gcgatgccgc
      841 ctgcgccttg ccgcgtcctt cggcgagcgc ctcggcaaag gtgccgaaca gcaccgtcag
      901 ccacagccag atgacgagct ggagcttgaa gccggtcgcg agaccgtcct gcccgacgac
      961 gagcaacacc gtcagcagcg cggcgacgac cgcggtcgtg aacatcaccg ggttgcggat
     1021 cagttccctg gggttgagtt tgcggaaggc atcgccgatc gccggaacga tcagatctgc
     1081 cgtgaacagg gatttttgtg cggaccgagc catgtgcatg gatccttaaa agagctggcc
     1141 gttgatcatc gcgagatgat cggcgatcgg accgagcgcg aggctgggaa gaaatgtgag
     1201 accaccgacg atcagcacga tgccgacgag caaacccgtc cacagcagcc ccgtggtcgg
     1261 gaagctgccc gccgttgccg gcgtatattt cttcgccgcc agaccgcccg cgatcgccag
     1321 catcgggatg atgatgaaga aacggccgat ccacatcgcg acgcccagca tgccgttata
     1381 gaaaggcgtg ttggcggtga gacccgcaaa ggccgacccg ttgttcgcga cggcgctggt
     1441 gaaggcgtag agaatctcgg agaagccgtg cggccccttg ttgagcggac ccgccagccc
     1501 cgcctcggtg accgatgcga tggcggtgaa gccgaggatg atcagcggca gcaccgcgat
     1561 cgcgagcacc gcgagcttca cctcgcgcgc ctcgatcttc ttaccgacat attcgggggt
     1621 gcgcccgacc atcaggccgg ccacgaagat cgcgaggatc gcgaagagca ggaagccata
     1681 gatgcctgcg ccgacgccgc cgatcacgac ttcaccaagc tgcatgttga acagcgggat
     1741 catgccgccc aaggcggtga agctgtcgtg catcgcgttc accgcgccgc acgacgcggc
     1801 ggtggtgacg acggcaaaga gcgccgactg ggcgatgccg aagcgcactt ccttgccctc
     1861 catattgccg cccggcacgc cgagattgtg gaggatcgga ttgccggcgg cttcctgcca
     1921 gtacgtgatc gccgcgcccg cgacgaacaa tgtcatcatc gcggcgagga tcgcccagcc
     1981 ttgccgctgg ttgccgacgg cccgcccgaa ggtccaggtc agcccgacgc cgatcaggaa
     2041 gatcgagagc atctggacga ggttggtgag cgcggtcgga ttttcgaacg gatgcgccga
     2101 gttgacgttg aagaagccgc caccgttggt gccgagcatc ttgatcgcct cctgcgacgc
     2161 gaccggaccg agcgcgagcg tctgcttcgc gccctccagc gtcgtcacgt cgaccgaacc
     2221 cgccagcgtc tgcgggacgc cgctcgcgat atagaaggtc gcgatgacga cgcagatcgg
     2281 cagcagcaga tagagagtga cgcgcgtcat gtcggcccag aaattgccga tcgtcgtcgc
     2341 gctgcgacgc gcaaagccgc ggaacagcgc aaaagcgagc gcgatgcccg tcgccgccga
     2401 caggaagttg tgaatggtga gcccgagcat ctgcgacagg ttcgacatcg tcgattcgcc
     2461 accatagctc tgccagttgg tgttggcggt gaagctgatc gcggtattga acgcgaggtc
     2521 cgcactcgtc ccgtcatagc cgagcggatt gagcggcagg acgccctgca accgcagcac
     2581 cgcataggta aagagcagca gcgccgtgtt gaagatgagc atgtggaccg cgtagcggcg
     2641 ccagccctgt tcttcatcgg ggttgatgcc cgacaggcga tagaagcccg tttcgaccgg
     2701 gccgagcacg cggtggagcg gcgtgcggcg tccttcgtag agcgcgaaga gccactggcc
     2761 catcggcttg cacagcaaaa gcaggatgcc gacgaaagcg gcgatcaaaa gccatccctg
     2821 aaaggtcatg gcgcgcctcc cctaaaagcg ttcgggccgc gcgagcacgg cgacgagata
     2881 gacgagaagg ccgagcgcgg tggcgcccgc gagccatagg tcgagagtca tggcatcacc
     2941 tcacgcgtcg tcgcaaaggc gcgcatagcc gaggcttgcc gccagaagcg cgaggatgat
     3001 gccgatccag agaagatcct gcatgatttg ctccagcaag gcgcccgcca caaaggtgac
     3061 gcgggtcgtc tgcggagcat caattagaat aggcgcgcgt ttgaattcga gagagtgaaa
     3121 tcagcccgcg catagtgttt gcatatgatt ttcgcgaaaa ccggcgtcgg tcctgacgtt
     3181 ccgtcagctc cgggccagtt cgcgcgcata gcccttgaac ccccacgtca ggatgattgc
     3241 ggcgagcggc aaggcggccg ccgtgacgat cgcaatcgaa tagcgcagcg ccgattcgtc
     3301 ctggaacaaa ttgtcggtga gcagtgccat cagcatcgga ccgaagccga ggccgaccag
     3361 attgacgaca aagaaataga gcgcggtcat ccgcgcgcga tattcgttcg gcgtgatcag
     3421 ctgcaaggcg acgccgccga cgccgtgcat cgtcagacac gggaaggcca gcgccagcat
     3481 cgcgaacgcg agcgcggggt cgggcatcaa cgccatcgcc gtcaagggcg ggccgccgag
     3541 caggatcgac aggcgcatca cccgcaaatg cgcgtcaccc acccccttgg cgaacaatcg
     3601 gtcggcgagc gcgccacccg cgatcaaccc gagggcgccg ccgatcgcca tgatgacgcc
     3661 gaaccacaag cccgcttgcg caacgtccat gccatagctg cgcgcgaaga aggtcggtat
     3721 ccacgcgatc gcgccataca tgacgagacc gatcagcgac atgccgccga acagcgcgcc
     3781 gagcgccgcc cgctgtccac cgatgtgcgc gacaagaccg ggtgcgccgg ccgccggcgc
     3841 atccggcgtc acagc
//
LOCUS       DATMSU010000506         7010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_169081,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000506 DATMSU010000000
VERSION     DATMSU010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169081"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1100
                     /gene="sseA"
                     /locus_tag="VN034_13380"
     CDS             258..1100
                     /gene="sseA"
                     /locus_tag="VN034_13380"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HWV61648.1"
                     /translation="MDALVSTDWLERELGASDLRVVDATKFTADEGRDARAEYETGHI
                     PGAIFMDLAELIDPSSNIDNMAPSAEKFASRMQSLGLGDGSRIVLYDDSPLKSAARAW
                     WLLKLFGAHDVALLDGGLAKWKAEGRALEMGKQTLRHRHFTVWRDAKAVRTKDQMVAN
                     VDSGAEQVVDARPAARFTGEERDPRPGVAPGHIPNSRSLPHGELFNADGTWKRGDDLK
                     AAFDAAGVDLDKPLVTTCGSGMTATVVAFGAHLLGKDDVAVYDGSWVEWGADATTPKA
                     TGAA"
     gene            1277..2482
                     /gene="metC"
                     /locus_tag="VN034_13385"
     CDS             1277..2482
                     /gene="metC"
                     /locus_tag="VN034_13385"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HWV61649.1"
                     /translation="MSNSDDDSLRPATKLVQGGRRPEWTGDPRLGGGIVNPPVWRAST
                     ILYDNIADLKARGHATHDKLYYGRRGTPTVWALADALTQMEEGAEGTLLYPSGVAANS
                     AGLLALLSPGDHLLMVDSAYEPTRAFCNGMLARLGVETTYYDPLVGAGIADLIRPETR
                     LIFLESPGSLTFEVQDIPAIARIARARGVLTMLDNTWATPLLFPALAHGVDVTMMSLT
                     KYVGGHSDAMMGSLTATKAVWPKLRSAAYQLGQSVSPDDCALMLRGLRTLEVRIQRQG
                     ENGLAVANWLANRAEVGRVLHPALLDDPGHAIWARDFSGASGLFGFTLKGADEAARTR
                     FIDSLAHFGIGFSWGGYESLVVPSDPQTIRTATRWTDPDPLVRLSIGLEDPVDLIADL
                     ERGFAAMRG"
     gene            2488..3420
                     /locus_tag="VN034_13390"
     CDS             2488..3420
                     /locus_tag="VN034_13390"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWV61650.1"
                     /translation="MDGLSEATVRAIGTLKAIGIDVGSYRLSLYSLFSTVIVAILLYV
                     AVRVALRSIKWLLRRNTQIDATQRLLAEKLIAIALFVFAMLFGIDLLGIDLTALAVFS
                     GAAGLAIGFGLQKTVGNLIAGIILLMDRSIKPGDIVVVGDGSAMGGTSLPGGMPNVGR
                     VAKIGVRAVNVITRDGKKHLIPNELLMTQAVENWSFASPEVRVRMRVPVGYDCDLRLA
                     QRLMVETAKENPRILAEPEPVVWITAFGERAVEHELRYWISDPEAGLGNIQGEVFLGV
                     WDRFKEAGIRIPYPRTDVRMVGAPDQDPAPTDAN"
     gene            complement(3417..3953)
                     /gene="ppa"
                     /locus_tag="VN034_13395"
     CDS             complement(3417..3953)
                     /gene="ppa"
                     /locus_tag="VN034_13395"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWV61651.1"
                     /translation="MRIDLIPAGDNPPDSLNVLIEVPIGGEPVKYEFDKASGALFVDR
                     FLHTPMRYPANYGFVPHTLGEDGDPLDALVVARSPIVAGAVVKCRPIGVLNMVDDAGG
                     DEKLLCVPVNKTFPYYDKVATYKDMPEIVLQQIEHFFTHYKDLEPGKWAKLNGWGDVD
                     EAKRIIVECIERAKKVGI"
     gene            complement(4087..6021)
                     /locus_tag="VN034_13400"
     CDS             complement(4087..6021)
                     /locus_tag="VN034_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HWV61652.1"
                     /translation="MKKALFVAAAVIAAPIAFSPIHAQEAVSRPQPVVQPLTIPLPAD
                     KPYPGTMQLKVDATDVARGIFHVRQTIPVAKAGKLTLLYPEWLPGKHAPRGAIAEMAG
                     FTASAGGKPLVWTRQPTDVYAFDIDVPAGTKSIDVTFDFLSPTRTSEGRVVVTPAMMN
                     LQWEQVSLYPAGYFTRNIPVQLDVTLPEGWTGASALDGLKLSGNRYSFAATNYETLVD
                     SPMFAGRHFRKWDLGNKVTLNVVADEAKYLEASPDHIARHAALVSEAVALFGVRHFDR
                     YEFLLALSDELGGIGLEHHRSSENSRNPDYFTKWNDNDSERGLLPHELVHSWNGKYRR
                     PDKLWTPDYRTPMQGNLLWVYEGQTSFWDLVLAARSGMQSKEMVLAEWATNAGYYSVQ
                     PGREWRSVEDTTLDPVIAARKPKPFSSWSRSEDYYNEGSLMWLEADMLIRGATNNARS
                     LDDFARAFFGGREGDWGQDTYDFDDVVAALNAVHPYDWASFLHERMQTSGRPAPTGGI
                     ERAGYRLVWRATPNVFDRDRMAQAKNADLTHSLGMTVDKDGVASGILWNGPAFKADII
                     NGTKIVAVGGLAYSRERIEAAVQAATDGKTPVRLLVERGGRYRNIAIDYHGGLRWPHL
                     EKSGSGPDWFDRLLAPRRAL"
     gene            6117..>7010
                     /gene="hisS"
                     /locus_tag="VN034_13405"
     CDS             6117..>7010
                     /gene="hisS"
                     /locus_tag="VN034_13405"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWV61653.1"
                     /translation="MSKDKSAKIPTPQAVRGTQDMLGDFADRFAYVVEAFERVRRLYG
                     FKRIEVPVIEPTAVFARSLGETTDVVSKEMYSFEDRGGESITLRPEFTAGIARAYVTN
                     GWQQFAPLKVATHGPLFRYERPQKGRYRQFHQLDAEVLGSDSPLADAELLVFADQLLK
                     ELGIAEGVTLTLNTLGDAASRDAWRAALVEHFEAHRGDLSEDSLARLDKNPLRILDSK
                     DPKDRPVADSAPDIDAYLTSEAQDFFGAVTAGLDAADVAWERNARLVRGLDYYRHTAF
                     EFVTDRLGAQGTVLGGGRYDGL"
BASE COUNT         1172 a         2253 c         2363 g         1222 t
ORIGIN      
        1 tgccgctcga ccgggcttcc tatgccgtcg accggcccgg cgcgcgcgcg accgcgatgc
       61 ggcgcagcca ataagtcccg cttaaaacgg cgctgctttt ctgttgcagt gcagcatcgg
      121 ctgcggcagg acggaccccg cttgaaaaag caggggtggg ggcataggct gactatcccc
      181 ggaaagaagc cacagcagga tggccgggcg agggacaggc aaacaaggtt cccgaccggt
      241 cgtaacagga gcatgacatg gacgccttgg tctcaaccga ctggctggaa cgcgaactgg
      301 gggcatccga cctgcgggtc gtcgatgcga caaaattcac agccgacgag gggcgcgacg
      361 cccgcgccga atatgagacg ggccacatcc ccggcgcgat attcatggac ctggccgaac
      421 tgatcgatcc gtcgagcaac atcgacaata tggccccgtc cgccgaaaaa tttgctagcc
      481 gcatgcagtc gctcgggctc ggcgacggca gccgtatcgt gctttacgac gacagcccgc
      541 tgaagagcgc ggcgcgcgcc tggtggcttt tgaagctgtt cggcgcgcat gacgtcgcgc
      601 tcctcgacgg cggtctcgcc aagtggaagg ccgaagggcg cgcgctcgaa atgggcaagc
      661 agacgctgcg ccaccgccac ttcaccgtgt ggcgcgacgc gaaggcggtg cgcaccaagg
      721 accagatggt tgcgaacgtc gacagcggcg ccgagcaggt cgtcgatgcg cgcccggcgg
      781 cgcgctttac cggcgaagaa cgtgatccgc gtcccggtgt cgcgccgggc catatcccga
      841 actcgcgcag cctgccgcac ggcgagttgt tcaacgctga cggtacgtgg aagcgtggcg
      901 acgatctgaa ggccgcgttc gacgccgccg gagtcgatct cgacaagccg ctcgtcacca
      961 cctgcggcag cggcatgacg gcgacggtcg tcgcgttcgg cgcacacctg ctcggcaagg
     1021 atgacgttgc ggtctatgac ggtagctggg tcgaatgggg cgccgacgcg accaccccga
     1081 aggcgaccgg cgcggcctga aaacaaaaac gtcgcccccg cgaaggcggg ggccgcggtc
     1141 gtccttactc ggcgccgaag gcttagactt acagcggccg cgttcagagg cacgtgactc
     1201 tgaaacgccc ttcgcggggg cgacggagtt tcgccggcct ctggattttg gccgctgctc
     1261 cgctaacaag gcggggatga gcaacagcga cgatgacagc ctccgccccg cgaccaaact
     1321 cgtgcagggc gggcggcggc ccgagtggac cggcgatccg cggcttggtg gcgggatcgt
     1381 caatccgccg gtctggcgcg cctcgacgat cctttacgac aatatcgccg acctcaaggc
     1441 gcgcggccat gcaacgcatg acaagcttta ttacggccgg cgcgggacgc cgacggtatg
     1501 ggcgctcgcc gatgcgctga cgcagatgga agagggggcg gaagggaccc tgctttatcc
     1561 ctcgggggtc gccgccaatt cggcggggct gctcgcgctg ctctcgccgg gcgatcacct
     1621 gctgatggtc gacagtgcct atgaaccgac gcgggccttt tgcaacggca tgctcgcgcg
     1681 gctcggggtg gaaacgacct attatgatcc gctcgtcggg gcgggaatcg ccgacctcat
     1741 ccggcccgaa accaggctga tcttcctcga atcgccgggc agcctgacct tcgaggtgca
     1801 ggatattccc gcgatcgcgc ggatagcgcg cgcgcgcggg gtgctgacga tgctcgacaa
     1861 cacctgggcg acgcccttgc tgtttcccgc gctcgcgcac ggcgtcgacg tcacgatgat
     1921 gagcctcacc aaatatgtcg gcgggcacag cgacgcgatg atgggctcgc tgaccgccac
     1981 gaaggctgtc tggccgaagc ttcgcagcgc ggcctatcag cttggccagt cggtttcgcc
     2041 cgacgattgc gcgctgatgc tgcgcgggct gcgcacgctc gaggtacgga tacagcggca
     2101 gggcgaaaat ggccttgccg tcgcgaactg gctggcgaac cgcgccgagg tcggccgcgt
     2161 gctgcacccc gcgctgctgg acgatcccgg tcatgcgatc tgggcgcgcg acttttcggg
     2221 cgccagcggg ctgttcggct tcacgctgaa gggcgccgac gaggcggcgc ggacgcgctt
     2281 catcgattcg ctcgcccatt tcggcatcgg gttcagctgg ggcggctatg aaagccttgt
     2341 cgtgccgagc gatccgcaga cgattcgcac cgccacccgc tggaccgacc ccgacccgct
     2401 cgttcgcctg tcgatcgggc tcgaggatcc ggtcgacctg atcgccgatc tcgaacgcgg
     2461 ctttgccgcg atgcggggtt gagcgcggtg gacggcctgt ccgaagccac cgtccgcgcg
     2521 atcggcacgc tgaaggcgat tgggatcgac gtcggctcgt accggctgtc gctctacagc
     2581 ttgttttcga cggtcatcgt cgcgatcctg ctctatgtcg cggtgcgggt cgcactgcgc
     2641 tcgatcaaat ggctgcttcg ccgcaacacc cagatagacg cgacgcaacg ccttctggcc
     2701 gaaaagctga tcgcgatcgc gctcttcgtc ttcgcgatgc tgttcggcat cgacctgctc
     2761 ggcatcgacc tcaccgcgct ggcggtcttt tcgggcgccg cgggtctcgc gatcggcttc
     2821 gggctgcaaa agaccgtcgg caatctgatc gcggggatca tcctgctgat ggaccggtcg
     2881 atcaagccgg gcgacatcgt cgtcgtcggc gatggcagcg cgatgggcgg caccagcctg
     2941 cccggcggga tgcccaatgt cgggcgcgtc gccaagatcg gggtgcgcgc cgtcaacgtc
     3001 atcacccgcg acggcaagaa gcatctgatc ccgaacgagc tcttgatgac gcaggcggtc
     3061 gagaattgga gctttgccag tcccgaggtg cgggtgcgga tgcgtgttcc ggtcggctat
     3121 gactgcgacc tccgcctcgc gcagcggctg atggtcgaga ccgcgaagga aaatccgcgc
     3181 atcctcgccg agcccgaacc ggtcgtgtgg atcacggcgt tcggcgaacg cgcggtcgag
     3241 catgagctgc gctactggat ttcggacccc gaggcggggc tcggcaacat tcagggcgag
     3301 gtgtttctgg gggtgtggga ccgcttcaag gaggcgggca tccgcattcc ctatccgcgc
     3361 accgatgtgc ggatggtcgg ggcgccggat caggacccgg cgcccaccga cgcaaattag
     3421 ataccgacct tcttcgcgcg ttcgatgcat tcgacgatga tgcgcttcgc ctcgtcgaca
     3481 tcgccccagc cgttgagctt cgcccatttg ccgggctcga ggtccttgta gtgggtgaag
     3541 aaatgctcga tctgctgcag cacgatctcg ggcatgtcct tgtaggtcgc gaccttgtcg
     3601 taatagggaa aggtcttgtt gacgggaacg cacagcagct tctcgtcgcc gccggcatcg
     3661 tcgaccatgt tgagcacgcc gatcgggcgg cacttgacca ccgcgcccgc gacgatcggc
     3721 gagcgcgcga cgaccagggc atccagcggg tcgccatcct cgcccagcgt gtgcgggacg
     3781 aagccgtaat tggcggggta gcgcatcggc gtgtgcagga agcggtcgac gaacagcgcg
     3841 cccgacgcct tgtcgaactc atatttcacc ggctcgccgc cgatcggcac ttcgatcagg
     3901 acgttgaggc tgtcgggcgg attgtcgccg gcgggaatca ggtcaatgcg catgggagat
     3961 gtcagtcctt tgtttttgat tcgggcgccc cttagccctt cgcatcgcgt gaagtcgaga
     4021 cacagcgaag ccgcgcggcg acgaccggtg tcttctctac gctcgaaacg gacggctgcg
     4081 gcgcgtttaa agcgcccgcc gcggcgccaa tagccgatcg aaccagtcgg ggccactgcc
     4141 gcttttttcg aggtgcggcc agcgcagtcc gccatgatag tcgatcgcga tattgcgata
     4201 acgcccgccg cgctcgacga gcagccgcac cggcgtcttg ccgtcggtgg cggcctgcac
     4261 ggccgcctcg atccgctcgc ggctgtaggc gagcccgccg accgcgacga tcttcgtgcc
     4321 attgattatg tcggccttga aggcgggacc gttccacagg atgccgctcg cgacgccatc
     4381 cttgtcgacc gtcatcccca gcgaatgggt caggtcggcg ttcttcgcct gcgccatacg
     4441 gtcacggtcg aagacgttcg gtgtggcgcg ccacacgagc cggtaacctg cgcgctcgat
     4501 cccgccggtg ggggcgggac gcccgctcgt ctgcatccgc tcgtgcagga agctcgccca
     4561 gtcataggga tgaaccgcgt tcagcgcggc gacgacatcg tcgaagtcgt atgtatcttg
     4621 cccccagtcg ccttcgcgtc cgccgaaaaa ggcgcgcgcg aaatcgtcga ggctcctggc
     4681 gttgttcgtc gcgccgcgga tcagcatgtc ggcctcgagc cacatcagcg acccttcgtt
     4741 gtaataatcc tcgctccgcg accagctcga aaagggcttg ggcttgcgcg cggcgatcac
     4801 tgggtcgagg gtcgtgtcct cgaccgagcg ccactcgcgc cccggctgca cgctgtagta
     4861 accggcattg gtcgcccatt cggcgagcac catctccttc gactgcatcc ccgaccgcgc
     4921 ggcgagcacg aggtcccaga agctcgtctg cccttcgtaa acccacagca gattgccctg
     4981 catcggggtg cgataatcgg gggtccacag cttgtccggc cggcgatatt tgccgttcca
     5041 gctgtggacg agctcgtgcg gaagcagccc gcgctcgctg tcattgtcgt tccatttggt
     5101 gaaatagtcg gggttgcggc tgttctcgct cgaacgatga tgttcgagcc cgatcccgcc
     5161 gagctcgtcg ctgagcgcga gcagaaattc atagcggtcg aaatggcgga cgccgaacag
     5221 cgcgacggct tcggacacca gcgcggcgtg gcgcgcgata tggtcggggc tcgcctccag
     5281 atatttcgcc tcgtcggcga cgacgttcag cgtcaccttg ttgccgaggt cccatttgcg
     5341 gaaatgacgc cccgcgaaca tcgggctgtc gacgagggtc tcgtaattgg tggcggcaaa
     5401 gctgtagcgg ttacccgaca gtttgagccc gtcgagcgcc gatgcgccgg tccagccttc
     5461 gggcagcgtc acgtcgagct gcaccggaat attccgtgtg aaatagcccg cgggatagag
     5521 gctcacctgt tcccattgca ggttcatcat cgccggggtg acgacgacgc ggccctcgct
     5581 cgtgcgcgtc ggcgacagga agtcgaaggt gacgtcgatg ctctttgtgc ccgcggggac
     5641 gtcgatatca aaggcgtaga cgtcggtcgg ctggcgtgtc cagacgagcg gcttgccgcc
     5701 cgcgctggcc gtgaaaccgg ccatttcggc gatcgcgccg cgcggcgcat gcttgccggg
     5761 cagccattcg ggatagagca gggtgagctt gcccgccttg gcgacgggga tcgtctggcg
     5821 gacgtggaag atgccgcgcg ccacgtcggt cgcatcgacc ttcagctgca tcgttcccgg
     5881 atagggcttg tcggcgggca gcgggatggt cagcggctgg acgaccggct gggggcggct
     5941 gaccgcttct tgtgcgtgga tgggggaaaa ggcgataggg gcggcgatga ccgccgctgc
     6001 gacgaacagt gcttttttca tgaggagcac ctctgccgcc aaatagcgcg aaggtccagc
     6061 ggcggctctg caagcgcgct ttgcctttcg acgaaaaacc attagacgcc gccgccatga
     6121 gcaaggacaa atccgccaaa atcccgaccc cgcaggccgt gcgcggcacg caggacatgc
     6181 tcggcgattt cgccgaccgt ttcgcttatg tcgtcgaagc gttcgagcgg gtgcgccgcc
     6241 tctatggctt caagcgcatc gaggtgccgg tgatcgagcc gaccgcggtg ttcgcgcgtt
     6301 cgcttggcga gacgaccgac gtcgtctcga aggaaatgta ttcgttcgag gatcgcggcg
     6361 gcgaatcgat taccttgcgc cccgaattca ccgcggggat cgcgcgcgcc tatgtgacga
     6421 acggctggca gcagttcgcg ccgctcaagg tcgcgacgca cgggcctttg ttccgctacg
     6481 aacggccgca aaaggggcgc tatcgccagt tccaccaatt ggacgcggaa gtgctgggca
     6541 gcgacagccc gctggccgat gccgaattgc tggtgttcgc cgaccagttg ctgaaagagc
     6601 tggggatcgc cgagggcgtg acgctgacgc tcaacacgct cggcgatgcc gcgagccgcg
     6661 acgcatggcg cgcggcgctg gtcgagcatt tcgaagcgca ccgcggcgac ctcagcgaag
     6721 acagcctcgc gcggctcgac aagaacccgc tgcgcatcct cgacagcaag gacccgaaag
     6781 accgcccggt cgccgacagc gcgcctgaca tcgatgccta tctgacgagc gaggcgcagg
     6841 atttctttgg tgccgtcacc gccggcctcg atgccgcgga cgtcgcttgg gagcgcaacg
     6901 cgcggctcgt gcgcgggctc gactattatc gccacaccgc gttcgaattc gtcaccgacc
     6961 gcttgggcgc gcagggcacg gtgctcggcg gggggcgcta cgacgggctg
//
LOCUS       DATMSU010000507         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_9046, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000507 DATMSU010000000
VERSION     DATMSU010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9046"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VN034_13410"
     CDS             <1..803
                     /locus_tag="VN034_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWV61654.1"
                     /translation="QVVGRYQPLLADQAIGKQEYDAAVAAQRTAEADVAAAQANLESA
                     RLNLGYASVTAPISGRARRAEVTEGALVSASQGTLLTTIEQIDRVYVNFGQSSSDLLA
                     IRGDMGSGKVSVPELERVEVQLILEDGSAYPVTGHLDFLDLSIDEATGTAALRAEFPN
                     PNSALLPGQFVRARIFAGNRADGVLIPQRAVKLAEDAASVMVLDAKNVATPRPVKLGT
                     MVAGQWAILEGLKPGDRVIVDGLQKVQPGAPVRIAQPKGAAPTPAAKR"
     gene            810..3974
                     /locus_tag="VN034_13415"
     CDS             810..3974
                     /locus_tag="VN034_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWV61655.1"
                     /translation="MTPRFFIDRPIFSWVIAIGILLAGIIALRGLPVEQYPSVAPPSL
                     TISVSYPGADASTLEQNVTQVIEQELNGVEGFLYMASTSESNGTASITLTFEAGTDID
                     NAQMEVQNRLRRVEQRLPEDVRRQGISVTEANSGFLLIVAITSKTGNTDPMEINNFAN
                     TRVLDELRRVNGVGNVQAFAPEYAMRIWLDPQKLASYNLSAAEALGAVQEQNSQTPGG
                     QLGDQPIAKGAQLNAVITTQGRFTKPEQFESIILRANPDGSAVTLGDVGRVELGAASY
                     LFSSELNGKPMAGLAVQLTPGANALSTAEGIRAQMEELAKGFPPDVTWSIPYDTTPFI
                     SLSIEEVVKTLAEAMLLVFLVMFLFLQNWRATVIPTVVVPIALAGACLGLWMFGFSIN
                     VLTLFGMVLAIGILVDDAIVVIENVERIMNEEHLPPYEATVKAMGQITSAIVGITLVL
                     IAVFIPMAFFPGSTGGIYRQFSMTLAISIAFSALLALTLTPALCATLLKPHDQSARKG
                     RIGAFFDRFFGRFNDWFGRTTDRYQGGVGKMLAAPLRWLGVFVAMVAITALLFFRLPG
                     SFLPQEDQGYLITVIQAPPGATTQRTNEATKQVKAFFAEQPQVANVVSVNGFSFFGQG
                     QANAIMFTPLKPWDERKGDENSADAIAGKAMGAFMGIKEAFVFSLSPPSIPELGTSSG
                     FTFKLQDRGGNGRDALIAARNQMLGGAMQSKLLANVRPEGQEDAPVLKVDIDRIQARA
                     LGLSIGDVNATLAISFGSAYANDFTREGRVLRVLLQADAASRMTPQDVLDLRVRSANG
                     GMVPFGSFSTAEWSAEAPQLQRYNGYPAMTISGEPAPGQSTGEAMAEMERLAQQLPAG
                     FAFEWTGISYEEKQSAGQIGMLLGLSLVVVFLLLAALYESWSVPVAVLLVVPLGVLGA
                     VLFSMLRGLSADIYFNVGLITIIGLAAKNAILIVEFAIEQEAEGKSTLDAVMEAVKLR
                     LRPIIMTSLAFILGMVPLVIASGAGAASRIAVGSGVMGGMIAATLLGIFFIPLFYLSV
                     RKWLSRKRPPAPAEKGHHEEPGHA"
BASE COUNT          685 a         1383 c         1340 g          699 t
ORIGIN      
        1 ggcaggtcgt cggccgttac cagccgctgc tcgccgacca ggcgatcggc aagcaggaat
       61 atgacgccgc ggttgccgcg cagcgcacgg ccgaagccga cgtcgccgcc gcacaagcca
      121 atctggaatc ggcgcgcctg aacctgggct atgcgtcggt caccgcgccg atttccggcc
      181 gcgcgcgccg cgcagaggtg accgaaggcg cactggtcag cgcatcgcaa ggcacgctgc
      241 tgacgacaat cgagcagatc gatcgcgtct acgtcaattt cggccagtcg agttcggacc
      301 ttctggcgat ccgcggcgac atgggttcgg gcaaggtcag cgtgccggaa ctcgaacgcg
      361 tcgaggtcca gctgatcctc gaggacggca gcgcctatcc ggttaccggc cacctcgact
      421 tcctcgacct gtcgatcgac gaggcgacgg gcaccgcggc gctgcgcgcc gaattcccca
      481 atcccaactc ggcgctgctt cccggtcagt tcgtgcgcgc gcgcatcttt gcgggcaatc
      541 gcgccgacgg cgtgctgatc ccgcagcgcg cggtcaagct ggcggaggat gcggcgagcg
      601 tgatggtgct cgacgccaag aatgtcgcga cgccgcgccc ggtcaagctg ggcacgatgg
      661 tcgcgggcca gtgggcgatc ctcgaaggac tgaagcccgg cgaccgcgtg atcgtcgacg
      721 ggctgcaaaa ggtccagccg ggcgcgccgg tccgcatcgc gcagcccaag ggagcggcgc
      781 cgacccccgc ggcgaagcgc tgacctgaca tgaccccccg cttcttcatc gaccggccca
      841 tcttctcctg ggtcatcgcc atcggcatcc tgcttgccgg gatcatcgcg ctgcgcggcc
      901 tgcccgtgga gcaatatccg tcggtcgcgc cgccgtcgct gacgatcagc gtcagctatc
      961 cgggcgccga cgccagcacc ctcgagcaga atgtcacgca ggtcatcgag caggaactga
     1021 acggggtcga gggcttcctc tacatggcct cgaccagcga atcgaacggc accgcgtcga
     1081 tcaccctgac cttcgaggcc gggaccgaca tcgataacgc gcagatggag gtgcaaaacc
     1141 gcctgcgccg cgtcgaacag cgcctgcccg aggatgtccg ccgtcagggc atttcggtga
     1201 ccgaggccaa ttcgggcttc ctgctcatcg tcgcgatcac ctcgaagacc ggcaacaccg
     1261 acccgatgga gatcaacaac ttcgccaaca cgcgcgtgct cgacgagctg cggcgcgtga
     1321 acggggtcgg caatgttcag gcctttgcac ccgaatatgc gatgcgcatc tggctcgatc
     1381 cgcaaaagct cgcctcctat aacctctccg ccgccgaagc cctgggcgcg gtgcaggaac
     1441 agaatagcca gacgccgggc ggccagcttg gcgaccagcc gatcgccaag ggtgcgcagc
     1501 tcaacgccgt gatcacgacg cagggccgct tcaccaagcc cgagcagttc gagagtatca
     1561 tcctgcgcgc caaccccgac gggtcggcgg tgacgctggg cgacgtcggg cgcgtcgagc
     1621 tgggcgcggc gagctatctc ttttcgtccg agctcaatgg caaaccgatg gcgggcctcg
     1681 ctgttcagct gacccccggc gccaacgcgc tgtcgaccgc cgaaggcatt cgcgcgcaga
     1741 tggaggaact ggcgaagggc ttcccgcccg acgtcacctg gtcgatccct tatgatacga
     1801 cccccttcat cagcctgtcg atcgaagagg tggtgaagac gctggccgag gcgatgctgc
     1861 tcgtcttcct cgtcatgttc ctgttcctcc agaactggcg cgcgacggtg atcccgaccg
     1921 tcgtcgtgcc gatcgcgctc gcgggcgcgt gtctggggct ctggatgttc ggtttttcga
     1981 tcaacgtgct gacgctgttc ggcatggtgc tcgcgatcgg tatcctcgtc gacgatgcga
     2041 tcgtggtgat cgagaatgtc gagcgcatca tgaacgagga acatctgccg ccctatgagg
     2101 cgacggtgaa ggcgatgggg cagatcacct cggcgatcgt cggcatcacc ctcgtgctga
     2161 tcgcggtgtt catcccgatg gccttcttcc ccgggtcgac cggcggcatc tatcgccaat
     2221 tctcgatgac gctcgcgatc tcgatcgcct tctccgcgct gctcgcgctg acgctgacgc
     2281 cggcactttg cgcgacactt ttgaagccgc acgaccagag tgcccgcaag ggacggatcg
     2341 gggcgttctt cgaccgcttc ttcggccgct tcaacgactg gttcggccgc accaccgacc
     2401 gctatcaggg cggcgtcggc aagatgctgg cggcaccgct gcgctggctc ggcgtgttcg
     2461 tcgcgatggt cgccataacc gcactgctct tctttcgcct gcccggatcc ttcctgccac
     2521 aggaagatca gggctatctg atcacggtca tccaggcccc gccgggcgcg acgacgcagc
     2581 ggaccaacga ggcgaccaaa caggtcaagg ccttcttcgc cgaacaaccg caggtcgcca
     2641 atgtcgtgtc ggtcaacggc ttcagcttct ttggccaagg ccaggcgaat gcgatcatgt
     2701 tcacaccgct gaagccgtgg gacgagcgca agggcgacga gaacagcgcc gatgcgatcg
     2761 cgggcaaggc gatgggcgcg ttcatgggga tcaaggaggc gttcgtcttc tcgctgagcc
     2821 cgccatcgat ccccgaactc ggcacgtcga gcggcttcac cttcaagctg caggatcgcg
     2881 gcggcaacgg gcgcgacgcg ctgatcgccg cgcgcaacca gatgctcggc ggcgcgatgc
     2941 agagcaagct gctcgccaat gtccgccccg aggggcagga ggatgcgccg gtgctgaagg
     3001 tcgatatcga ccgcattcag gcgcgcgcgc tcggcctgtc gatcggcgac gtcaatgcga
     3061 cgctcgcgat cagcttcggc agcgcctatg ccaacgactt cacccgcgaa ggccgcgtgc
     3121 tgcgcgtgct gctccaggcc gacgcagcga gccggatgac accgcaggat gtgctcgact
     3181 tgcgcgtgcg cagcgcgaac ggcggcatgg tgccgttcgg atcgttcagc accgccgaat
     3241 ggtcggccga ggcgccgcag ctccagcggt acaatggcta ccccgcgatg acgatttcgg
     3301 gcgagcccgc gccgggccag tcgacaggcg aggcgatggc cgaaatggaa cggcttgcgc
     3361 agcagcttcc ggcgggcttc gcgttcgaat ggaccggcat ctcctatgag gagaagcagt
     3421 cggcggggca gatcggcatg ttgctcggcc tgtcgctcgt cgtcgtcttc ctgctgctcg
     3481 cggcgctcta cgaaagctgg tcggtgccgg tcgcggtgct gctcgtggtg ccgctcggcg
     3541 tgctgggcgc agtgctcttc tcgatgctgc gcgggctttc ggcggacatc tatttcaacg
     3601 tcggcctgat cacgatcatc gggctcgcgg cgaagaatgc gattctgatc gtcgaattcg
     3661 cgatcgagca ggaagccgag ggcaagtcga cgctcgacgc ggtgatggag gcggtcaagc
     3721 tgcgcctgcg tccgatcatc atgaccagcc tcgccttcat cctcggcatg gtgccgctcg
     3781 tcatcgcgag cggagccggc gccgcgagcc gcatcgcggt cggatcgggc gtgatgggcg
     3841 ggatgatcgc cgcaactttg ctcggcatct tcttcattcc gctcttctat ctgtcggtgc
     3901 gcaaatggct gagccgcaag cggccccccg cccctgccga gaaaggccat catgaggaac
     3961 ccggccatgc gtaacctgat cgccctcgcc gcggcgacga cgctcgccgg atgcgccaat
     4021 atggcgccgc cgcacgagcg tccgccgctg tcgaccgccc ccgcctatcc ggccgaattc
     4081 gccggcgatg tgacgctcgg gcagcgt
//
LOCUS       DATMSU010000508         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_36859, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000508 DATMSU010000000
VERSION     DATMSU010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36859"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..660
                     /gene="rimM"
                     /locus_tag="VN034_13420"
     CDS             166..660
                     /gene="rimM"
                     /locus_tag="VN034_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043027.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWV61656.1"
                     /translation="MADANRPVTLAAIAGAHGVRGEVRLKLFGEGAEALRAFSVFDAG
                     DRKLTLKSVRPANQGAVATFAEVTDRSAAEALRGAVLTVPRSALPALDEGEYYHHDLI
                     GLPCVSTDGSAIGHVAAVENFGAGDILEIEKPGGKRFMVPMNARAVPAWNADGVTVEA
                     AFVE"
     gene            complement(738..1007)
                     /locus_tag="VN034_13425"
     CDS             complement(738..1007)
                     /locus_tag="VN034_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61657.1"
                     /translation="MDFDDQLRRYFGTADIAAIHPEAYAAGTERMRVDFGLETNPGRR
                     FALWALMHMLGVAPDLDVAFEDEDERNTARDFMDMVDRAGKDEAG"
     gene            1277..>2499
                     /locus_tag="VN034_13430"
     CDS             1277..>2499
                     /locus_tag="VN034_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020817664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWV61658.1"
                     /translation="MKTSIEIGTDSTGQAVRIDVQELLATRLLVQGNSGSGKSHLLRR
                     ILEESAALVQQIVIDPEGDFVTLADAYSHVVINGGDYNEREIAAMGARIREHRASIIL
                     SLESLDIEAQMTCAAAFLNALFDAPREHWFPALVVVDEAQMFAPSVAGDVADNIRRAS
                     LGAMTNLMCRGRKRGLAGAIATQRLAKLAKNVAAEASNFLMGRTFLDIDMARAADLLG
                     MERRQAETIRDLERGHFLGLGPAISRRPIAVRIGSTQTSTRMGTHGLMPLPSAGGEDM
                     RSMLFTEMEAPAAPRVFAPEPEPVAASEVMERIAASEALHEDADAPPVFDIVEAAAEA
                     EQSDAVVIETLHEMLSDPDCAFQPESFLYQDFSVRCRMQKLKKVPLDLAAFRRRFALA
                     KGGVFDPAEPRWREAL"
BASE COUNT          419 a          818 c          856 g          406 t
ORIGIN      
        1 gccgaagccg tcgccgacga aaccccggcc gacgcgaccg ccgaagacgc gaccgcgatc
       61 gccgaggaag tcgccgaagg cggcccggtt gtcgaagccg acgctccggc cgccgaagaa
      121 gcgcccgctg aggaagccgg cgaagaaaag gccgaaggct aagccttggc cgacgcaaac
      181 cgccccgtca ccctcgccgc catcgccggc gcgcacgggg tgcggggcga ggtgcgtctc
      241 aaattgttcg gcgaaggcgc ggaagctctc cgcgcctttt ccgttttcga tgcgggggac
      301 cgcaaactca ccctcaaatc ggtgcgcccc gccaatcagg gcgcggtcgc aaccttcgcc
      361 gaggtcaccg accgcagcgc ggccgaagcc ttgcgcggcg ccgtgctcac cgtcccgcgc
      421 tcggcgctac ccgctttgga tgagggcgaa tattatcatc acgacctgat cggcctgccg
      481 tgcgtttcga ccgatggcag cgcgatcggg catgtcgccg cggtcgagaa tttcggcgcg
      541 ggcgacatcc tcgaaatcga gaaaccgggc ggcaagcgct tcatggtgcc gatgaacgcg
      601 cgcgcggtgc cggcgtggaa cgccgacggc gtgaccgtcg aggccgcgtt cgtcgaatag
      661 ggtatcgcct gcctgggtgg attgaagaca ttcccctccc tgtcagaaag gggagaggat
      721 atggatgcta cccgccgcta gcccgcttcg tctttccccg cccggtcaac catatccata
      781 aagtcgcgcg cggtgttgcg ctcgtcctca tcctcgaaag cgacgtcgag gtcgggggcg
      841 acgcccagca tgtgcatcag cgcccagagc gcgaagcggc ggccggggtt ggtttcgagg
      901 ccgaagtcga cgcgcatgcg ttcggtgccc gcggcatatg cctcggggtg gatcgccgcg
      961 atgtcggcgg tgccgaaata gcggcggagc tgatcgtcga aatccatgcc gtccttttgg
     1021 ccgactttgg tcgtttggca agtgaagcga agatcctccc ccttgatggg ggaggcagcg
     1081 agacttgcga acttgttcgc tagtcgcagt ggtgggggtg atgtgtcggc ctgacgcgac
     1141 ggtgcaagga cgcaccgtca cccccatcca actccgccta atcgcttcgc gacaaggctg
     1201 cgtatccttc ccccatcaaa ggggaaggtg tttgatccgg ttgtcattcg catgggcccc
     1261 cgatagggcg gccaccgtga agaccagcat cgagatcgga accgacagca ccgggcaggc
     1321 cgtgcgcatc gacgtgcagg aactgctcgc gacgcgcctg ctcgtccagg gcaattcggg
     1381 ctcgggcaag tcgcacctgc tacgccgtat cctcgaggaa agcgccgcgc tggtgcagca
     1441 gatcgtgatc gatcccgagg gcgatttcgt gacgctcgcc gatgcctaca gccatgtcgt
     1501 gatcaacggc ggcgactata acgagcgcga gatcgccgcg atgggcgcgc gcatccgcga
     1561 acatcgcgcg tcgatcatcc tcagtctcga atcgctcgac atcgaggcgc agatgacctg
     1621 cgccgcggcg ttcctcaatg cgctcttcga tgcgccgcgc gagcattggt tccccgcgct
     1681 ggtcgtggtc gacgaggcgc agatgttcgc gccgagcgtc gcgggcgacg tcgccgacaa
     1741 tattcgccgc gcgagccttg gtgcgatgac caacctgatg tgccgcgggc ggaaacgcgg
     1801 tctggctggg gcgatcgcga cgcagcggct cgccaagctc gcgaagaatg tcgccgcaga
     1861 ggcgagcaac ttcctgatgg ggcgcacctt cctcgacatc gacatggcgc gcgccgccga
     1921 tctgctcggc atggagcggc ggcaggccga gacgatccgc gacctcgaac gcgggcattt
     1981 tctcgggctc ggtcccgcga tctcgcggcg gccgatcgcg gtgcggatcg ggtcgacaca
     2041 gacctcgaca cggatgggca cgcacgggct gatgccgctt ccgtcggcgg ggggcgagga
     2101 tatgcgctcg atgctgttca ccgagatgga ggcgcccgcg gcgccgcgcg tcttcgcgcc
     2161 cgaacccgag ccggtcgcgg cgagcgaggt catggaacgg atcgcggcga gcgaagcgct
     2221 gcacgaggat gccgatgcgc cgccggtgtt cgacatcgtc gaagcggccg cggaggccga
     2281 gcagagcgac gcggtggtga tcgagacgct gcacgagatg ctttcggacc ccgattgcgc
     2341 cttccagccc gaatccttcc tctatcagga tttttcggtg cgctgccgga tgcagaagtt
     2401 gaagaaggtg ccgctcgacc tcgccgcctt ccgccgccgc ttcgcgctcg ccaagggggg
     2461 cgtgttcgat cccgccgagc cgcgctggcg cgaggcgct
//
LOCUS       DATMSU010000509        13482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_94172, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000509 DATMSU010000000
VERSION     DATMSU010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 13482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13482
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94172"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..221
                     /locus_tag="VN034_13435"
     CDS             81..221
                     /locus_tag="VN034_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61659.1"
                     /translation="MSNPPSASQGEPLQIGCRMQMLLLATFVSLFVAACWLIGTMMAN
                     PS"
     gene            237..479
                     /locus_tag="VN034_13440"
     CDS             237..479
                     /locus_tag="VN034_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61660.1"
                     /translation="MLREVAESSLRWTYSLEEILGLILVAAVVGAYSLMFAVTRKPRR
                     GWIVTAFVSFTLMAALISASPPESRLAAAKIAEAGM"
     gene            complement(591..959)
                     /locus_tag="VN034_13445"
     CDS             complement(591..959)
                     /locus_tag="VN034_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61661.1"
                     /translation="MNAASGVKRMKGNENSMAGRRPDWMRRRVMPAAQLTQKQPHARD
                     SDRSGQENVKDEACDWRLQIGFVMAIKDRQFAREPHVAHDYGFVDDRQAPKLADQGGE
                     FLLAQVGISIERFLDLLQRR"
     gene            complement(1057..1296)
                     /locus_tag="VN034_13450"
     CDS             complement(1057..1296)
                     /locus_tag="VN034_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61662.1"
                     /translation="MRDELIAILINVDEGAESTIADFVFGARVDKRTLAPRKWRFLMV
                     ILEQILPDARANILEEPSQAADDRIVAAYRMKRLP"
     gene            1315..2520
                     /locus_tag="VN034_13455"
     CDS             1315..2520
                     /locus_tag="VN034_13455"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HWV61663.1"
                     /translation="MADPASFRILIGGDACRKPYKASIFNISAMSFGALSANAIRALN
                     KGAKLGGFAHDTGEGSISSYHREHGGDLIWEIGSGYFGCRDENGAFEPGRFAEQAADP
                     QVQMIELKISQGAKPGKGGILPAEKVSPEIAMTRGIPMGRDCISPPGHSAFSTPMELV
                     HFIGRLRELSQGKPVGFKLCVGQPWEFMAIVKAMLKTGILPDYIVVDGAEGGTGAAPL
                     EFTDHLGMPLRESLLFVHNTLVGAGLRQKIKIGAAGKIVSAFDIATVMALGADWTNAG
                     RGFMFAIGCIQSLSCHTNQCPTGVATQDPIRQRALVVGDKADRVYNFHRNTLLALSEM
                     IAAAGLDHPSRLGPHHLVRRVSLTEIRLFSQLHIFLENGELLSGASDRDFYGAAWRLA
                     QPASFEMAA"
     gene            complement(2531..2953)
                     /gene="mnhG"
                     /locus_tag="VN034_13460"
     CDS             complement(2531..2953)
                     /gene="mnhG"
                     /locus_tag="VN034_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HWV61664.1"
                     /translation="MIQAPDLPLWAAIIVGLLVLGGASITLIGAAGLLRLHSFYERVH
                     APTLGATSGMAAILAASAICFSVLQARPVVHEILIALFVTLTTPVTLMLLARAAIYRD
                     RSEASASVPSDPDSDANRMIPAPNASTVKDEAGGHRRG"
     gene            complement(2950..3231)
                     /locus_tag="VN034_13465"
     CDS             complement(2950..3231)
                     /locus_tag="VN034_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HWV61665.1"
                     /translation="MTLLFLALTITASQILLGLAMGCAAFRMLRGPRAQDRILGLDAL
                     YLTAMLLLVTYGIQTGRTLYFEAALVIGLLGFTGTVALGRFLMRGEVIE"
     gene            complement(3228..3713)
                     /locus_tag="VN034_13470"
     CDS             complement(3228..3713)
                     /locus_tag="VN034_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HWV61666.1"
                     /translation="MIRRLIPFPLLTLSLLAMWILLTGFSPGHVLFGAAIAVMVSRTM
                     LSLRPERPTLRIGRASAKLAFIVLIDIVRSNVAVAKIILLRPPERQSHFIEFPTALRS
                     PDALATLAVIITATPGSLWVQHDAHRHIILIHVLDLVDEEQWIELIHTRYERLLLEIF
                     E"
     gene            complement(3710..5311)
                     /locus_tag="VN034_13475"
     CDS             complement(3710..5311)
                     /locus_tag="VN034_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HWV61667.1"
                     /translation="MTGWPHHLVITPIILPLMASALMLAFDERRRVLKRVLGLGTAAL
                     LVVNAAALLWLAGDGSGPLVYLLGNWAAPFGIVLVADRLSAMMLLLASVVGFTALFYA
                     IARWDRSGPRFHALFLLLLMGVNGAFLTGDIFNLFVFFEVLLAASYGLILHGSGTERT
                     SASLHYITINIAASLLFLLGVAMLYSVTGTLNLADLAMRVASTDAHDLALLEAGAAIL
                     GVAFLIKAGIWPLSFWLPRTYAAAAAPVAALFAIMTKVGIYVILRLSSLLFGDASGEA
                     AGFANEALLYGGLATMVFGIIGILSARQLTRVAGYYVLVSSGTLLAAIGIGGEALIAA
                     MLFYLLSSTLAVGALYLIIEPVERNADEEDMIAGIAEPVFDDEYVGAIEEVADEEGMV
                     IPGTIAILGGGFIFCALLLAGLPPLSGFIAKFAMIDALLTTSDVEPAGWALIGLIILS
                     GLATMMIMTRAGIDLLWTPGEDAPSRLSLVEVFPIGLLLAVCLGLMLWAGPVMGYLER
                     TSSALHQPAQYVAAVTDAPQTGTER"
     gene            complement(5308..5655)
                     /locus_tag="VN034_13480"
     CDS             complement(5308..5655)
                     /locus_tag="VN034_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HWV61668.1"
                     /translation="MEVVLALAVGVLTASGLWLVFRPRTFQVILGLSLLSYAVNLFIL
                     AAGRLRSDAPPIVGRDPASADPARYADPLPQALVLTAIVISFATTALLLVVLLASRGL
                     TGTDHVDGEDGEP"
     gene            complement(5655..8582)
                     /locus_tag="VN034_13485"
     CDS             complement(5655..8582)
                     /locus_tag="VN034_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HWV61669.1"
                     /translation="MDDTRLLLLVLILPFVGSTIAAFLPQRGRDVAASLTGFNALAGA
                     AMIASLYPIVITGRVARFEISWLPALDLNLTLRLDGFTWLFAMLVLIIGALVSFYARY
                     YMSATDPVPRFFAFLQAFMGAMLGILLSGNLIQIAFFWELTGLFSFLLIGYWHHNQSA
                     RDGARMALIVTGAGGVCLLVGLMLLGRAAGSYDVDVVLAARTAIQNDAAYLPALILIL
                     IGAFTKSAQFPFHFWLPHAMAAPTPVSAYLHSATLVKAGIFLLILLWPVLAGTESWFL
                     IVTGAGLATLLVGAWSAIFQQDLKGLLAYSTISHLGLITLLLGLGSPLGAVAAIFHTV
                     NHATFKASLFMAVGIIDRETGTRDLRLLSGLARFMPITATLAMVAAAAMAGVPLLNGF
                     LSKEMFLAEALEAHSGTLIDVALPYLATLASMFSVLYSVRFIHQIFFGPAPSGLSHEP
                     REAPPWIRLPIEILVLACLLVGVLPALTIGPFLATAVGSVLGARAPEYSLVVWHGFTL
                     PLLMSFAALLGGVALYILFRARLNATERAPFIGRLKGRRTFEAVLGAIVLAARALLRW
                     FGTKRLQVQLRLIIVVALVAGTLPFLRYGYSRGSVPDTLIDPVFALLWILGGACAVAA
                     AVLAKFHRLVALVLIGGAGLATCISFIWLSAPDLALTQLLVEIVTLVLLLLGLRWLPL
                     RLPDVWPAGSKPGTVGVRRGGDLLIALLGGTGMAALAYAMMTRPLPESLSRYFVEQAY
                     PGGGGTNVVNVILVDFRGFDTLGEITVLAIVALTVFTLLRRFRPAPESIEMPMQQHRQ
                     NAFDDSREGHARGDTLAHYLLVPRAIIQWLFPVILMFAIYLLMRGHDLPGGGFAGGIA
                     ASIAILLLYIANGTRAVEASLHIEPVRWISAGLLCAGGTGLAAWLFGYPFLTSHSRYL
                     TIPWIGDIPLATALLFDIGVFAVVVGTTVLILIAIAHQSIRAPRAKKPIAAPPATPAG
                     DSV"
     gene            complement(8896..9501)
                     /locus_tag="VN034_13490"
     CDS             complement(8896..9501)
                     /locus_tag="VN034_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61670.1"
                     /translation="MRQFDIDEALDRATREFWAKGYAGTTLDDLTQAMGIKRPSLYRV
                     FGNKEALFVRVVEHFESTYLAFVGDALASETPDLIVRRLLEGTVRACFGTGTPRGSLL
                     AHGAPAGSPEDEGVRILLAERIEVYERKLAASLARFQAGGTAPPGGDGDAASFLITHC
                     CGIALRAKAGASHDKLLAETDFVMAAFGFGTIEEPRQDNCV"
     gene            complement(10393..11346)
                     /locus_tag="VN034_13495"
     CDS             complement(10393..11346)
                     /locus_tag="VN034_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HWV61671.1"
                     /translation="MIGYFILLHRFPEQFKRMFRAIYAPGNSYVIHVDKGSGPVFAGD
                     IAAFLKTYEGVELIESKQALWGGYSLVDAELRGMKRLLKMNRDWTHYINLSGQDFPLK
                     SQAYIRSFLRAHPRRQFIRALDQRTMRPDTMNRVSHIFVEAIGRMFRTHLPRAFLKDA
                     TPYIGTQWKAVTRSFCHFAVHHPSAERFKTFYRRSFIPDEAFFQTLLMNSRGLGEVVN
                     DDLRTIDWVPDGDIKLRPRTFVTADALRLTLSPDLFARKFDAEEDPAIFDLLEAHLET
                     PAANIYASDRAAQPPPRSVGEDACMTDNAAEPHTPSPAGRK"
     gene            11702..12139
                     /locus_tag="VN034_13500"
     CDS             11702..12139
                     /locus_tag="VN034_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV61672.1"
                     /translation="MGKRWLRDMLRSRSKEARAMFNSRRILIVEDEPFVVIELVEAVE
                     RSGGAVVGPVPTVAEALALLDQHKIDAAILDVQLLDGEISPVALRLIEQGTPLVFQTG
                     TGLPVVLVELDIELAVVLKPVAPMIILELLLAEIMRQTARQVG"
     gene            complement(12215..12349)
                     /locus_tag="VN034_13505"
     CDS             complement(12215..12349)
                     /locus_tag="VN034_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61673.1"
                     /translation="MAKSQKRSNREIRKPKAAKGKPTTALAAKEASPVKLTAKLKDNG
                     "
     gene            complement(12466..12681)
                     /locus_tag="VN034_13510"
     CDS             complement(12466..12681)
                     /locus_tag="VN034_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61674.1"
                     /translation="MNLSLPEDEVRRLCLAQGVSISAIEPLQSGGTRLVCTSSAGAEE
                     MRLRLRGHLIEGSVVRHRFYRPSANEN"
     gene            complement(12690..12998)
                     /locus_tag="VN034_13515"
     CDS             complement(12690..12998)
                     /locus_tag="VN034_13515"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HWV61675.1"
                     /translation="MTSRIQNAEAPSPAEPTPTKGRSNSFFRKSRVPFALEDARRQGD
                     ISQLAYLVMGGRDAAVAFLNAENEELGGRPIALATASAEGYEHVVAAIRAWKSKRRSE
                     "
     gene            complement(13234..>13482)
                     /locus_tag="VN034_13520"
     CDS             complement(13234..>13482)
                     /locus_tag="VN034_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61676.1"
                     /translation="RAAGDRRPIPFMFATKENAEGTVMPFYGSQNVRGCSAQLKLRHG
                     NEAKQLVARQLEWARERGNGQEIAYWAAVLEDLASMPS"
BASE COUNT         2703 a         4072 c         4321 g         2386 t
ORIGIN      
        1 ccggtccggc gcgagatgct cgccctggtg acggtgctac gcgcgcgctc tgcgaccatc
       61 gagggcggcg aggacgcgcc atgagcaacc cgccgtccgc ctcgcaaggc gagccgctgc
      121 agatcggctg ccgaatgcag atgctgctgc ttgccacctt cgtctccctg ttcgttgccg
      181 cttgctggct gatcgggacg atgatggcga acccgtctta gaattggagt tagaccatgt
      241 tgcgcgaagt ggctgaatcg agccttcgct ggacttacag cctcgaagag atcctcggcc
      301 tcattctcgt cgccgcggtc gtcggagctt acagcctgat gttcgcggta acgcgtaagc
      361 cccggcgcgg ctggattgtc actgcctttg tgtccttcac gctcatggcg gcgctgatct
      421 cggcaagccc gcctgaatcc cgtctggccg cagcgaagat cgccgaagcc gggatgtagg
      481 tttggatgca ctgactgaca catcgatcat tcggggcttc cttggggagg ctcaaaggag
      541 catggacaat gagcaaatat gaatcacgga ccgagcggtg gcgaccccgg tcaccgacgt
      601 tgcaggaggt cgagaaatcg ctcgatcgat atcccgacct gggcgagcag gaactcgccg
      661 ccctgatccg ccaatttcgg agcctgtcgg tcgtcgacaa agccgtaatc atgggcgacg
      721 tgcggctctc gcgcaaactg gcggtctttt atcgccatca cgaacccgat ctgcaggcgc
      781 cagtcgcagg cctcgtcctt tacattctct tgcccgctgc ggtcgctgtc gcgggcgtgc
      841 ggctgtttct gggttaattg cgccgcaggc atcacccgcc gacgcatcca atctggcctt
      901 ctgccggcca tggagttttc gttgcccttc attcgtttca ccccgctcgc ggcgttcata
      961 gccgcctcgc ttatcgcaat tccgggactc cccgcggcgt gggcctgggc gttgttcgtc
     1021 accggcgcca cattatccgg tgtcgggctc tatgatctac ggcagccgct tcattcggta
     1081 cgccgcaact atccgatcgt cggccgcctg cgatggttct tcgaggatat tcgcccgggc
     1141 atccggcaat atctgttcga ggatgaccat gaggaaacgc cattttcgcg gagccagcgt
     1201 tcgcttgtct acgcgcgcgc caaaaacgaa gtcagcgatc gtgctttcgg caccctcgtc
     1261 gacgtttatg agaatggcta tgagttcatc gcgcactcga cgcggcccgt gccggtggcc
     1321 gatcccgcca gctttcgtat cctgatcggc ggcgatgctt gccggaagcc ctataaggca
     1381 tcgatcttca atatttccgc aatgagcttc ggcgcgctca gcgccaacgc gatccgcgcc
     1441 ctcaataaag gtgcgaagct tggtggcttc gcccacgaca ctggcgaggg ctcgatcagt
     1501 tcctatcatc gcgaacatgg tggcgatctc atttgggaaa tcggaagcgg ctatttcggt
     1561 tgccgggatg aaaacggtgc gttcgaaccc ggacgctttg ccgagcaggc cgcagatccg
     1621 caggtccaga tgattgaact caagatcagc caaggcgcga aacccggcaa gggcggcatt
     1681 ttgcccgccg aaaaagtatc tcccgaaatc gccatgaccc gcggtatccc gatgggccgc
     1741 gactgcatct cgccgccggg tcactcggcg ttcagcacgc ccatggagct cgtccacttc
     1801 attgggcgtc tgcgcgaatt gtcgcagggc aagccggtgg ggttcaagct ttgcgtcggc
     1861 cagccgtggg agttcatggc gatcgtcaag gccatgttga agaccggcat tctcccggac
     1921 tatatcgtcg tcgacggcgc cgagggcggt accggcgcgg caccgctcga attcaccgac
     1981 catctgggca tgccactccg cgagagcctg ttgttcgtcc acaacacgct ggtcggcgcg
     2041 gggcttcggc agaaaatcaa gatcggcgcc gcaggcaaga tcgtcagcgc cttcgatatc
     2101 gccacggtga tggcgctcgg cgccgactgg accaatgcgg gccggggctt tatgttcgcg
     2161 atcggctgca tccagtcact ctcctgtcat accaaccaat gtccgacggg ggtcgcgacc
     2221 caggatccga tacgtcagcg tgcgcttgtc gtgggcgaca aagccgaccg agtttacaat
     2281 ttccaccgaa atacgttgtt ggcgctgtcc gagatgattg ccgctgccgg gctcgatcac
     2341 cccagccgtc ttggaccgca ccatcttgta cgccgcgtca gtctcaccga aatcaggctg
     2401 ttctcacagc tgcacatctt cctcgagaac ggcgagctgc tgagcggtgc ctcggaccgg
     2461 gacttttacg gtgccgcgtg gcggctcgcc cagcccgcaa gcttcgagat ggcggcctag
     2521 ggtcctgacc ctagccgcgg cggtgcccac ctgcttcatc tttgacggtg ctcgcgttgg
     2581 gcgccgggat catccggttc gcgtctgagt ccgggtccga tggaacgctt gcgctggcct
     2641 cgctgcggtc gcggtagatc gccgcgcggg caaggagcat cagcgtaacg ggcgtagtca
     2701 gcgtgacgaa gagcgcgatc aatatctcgt ggacgactgg acgagcctgg agcacggaaa
     2761 agcagattgc cgaggccgcg aggatcgccg ccattcctga tgttgcacca agtgtgggcg
     2821 cgtgcacccg ctcgtagaag ctgtggaggc gcagcagccc ggccgctcca ataagggtga
     2881 tcgatgcgcc gccaagaaca agcagtccca cgataatcgc agcccatagt ggcaggtccg
     2941 gcgcctggat cattcgatca cctctccgcg catcaaaaac cggccaagtg ccaccgtgcc
     3001 ggtaaagccg agaaggccga taacgagtgc cgcctcgaaa tagagcgttc ttccggtctg
     3061 gattccatag gttacgagaa gcagcatcgc ggtaagataa agagcgtcga gccccaggat
     3121 gcggtcttgg gctcgcggtc cgcgaagcat gcgaaaggcg gcgcagccca tggcgagacc
     3181 gagcaggatt tgagacgccg tgatcgtcag ggccagaaac agcagggtca ttcgaatatc
     3241 tccagcaata ggcgctcata gcgcgtatgg atcagctcga tccattgctc ttcgtcgaca
     3301 aggtcgagca catggatgag aatgatgtga cgatgggcgt catgctgcac ccagagcgaa
     3361 cctggcgtcg cggtgatgat gacagcaagc gttgcgagcg cgtcggggct acgcaaggcc
     3421 gtcgggaact cgataaagtg cgactgccgc tcgggcggac gcagcagaat gattttcgcg
     3481 acggcgacgt tggaccggac gatatcgatc aagacgatga aggcgagctt cgccgaggcg
     3541 cggccgatgc ggagcgttgg gcgctcgggc ctgagcgaaa gcatcgtgcg cgacaccatc
     3601 accgcgatgg cggcgccgaa aagcacatgg ccgggcgaga agccagtcag caaaatccac
     3661 atcgccagca gcgaaagggt aagcagcgga aaagggatga ggcggcggat catcgctcgg
     3721 tccccgtctg cggtgcgtcg gtcaccgctg cgacatactg tgctggctgg tgcaacgcgg
     3781 atgacgttct ctcgagatat cccatgacgg gaccggccca cagcatgagg ccaagacaga
     3841 ccgccagcaa caggccgatc gggaacactt ccaccagact gagccgcgat ggcgcgtcct
     3901 cgccgggcgt ccagagcagg tcgatgccgg cgcgcgtcat gatcatcatt gtcgcgaggc
     3961 ccgagaggat gatcaggccg atcagcgccc agcctgccgg ttccacgtcg ctcgttgtca
     4021 gcaaggcgtc gatcatcgcg aacttcgcga taaatcccga aagcggcggc aggcccgcta
     4081 ggagaagcgc gcaaaatatg aaaccaccac cgaggattgc gatcgtgccc gggatgacca
     4141 ttccttcctc atccgcgacc tcctcgatgg cgccgacata ttcatcgtcg aacacgggct
     4201 cggcgatgcc tgcgatcatg tcctcctcgt cggcattgcg ttcaacaggc tcgatgatca
     4261 gatagagcgc gccgactgcg agcgtggagc tgaggagata gaaaagcatc gcggcaatca
     4321 gcgcctcgcc gccgatgcca atggccgcca gcaaggtgcc cgaggacacg agcacataat
     4381 agccggcgac gcgcgtgagc tgccgagccg agaggatgcc gatgattccg aagaccatcg
     4441 tcgcaaggcc gccgtacagt agggcttcgt tagcgaagcc ggcggcttcg cccgacgcat
     4501 cgccgaagag cagcgacgac aggcgcagaa tgacatagat gcccaccttg gtcatgatgg
     4561 cgaacagcgc ggcgacgggg gcggcggcgg cggcgtaggt ccgcgggagc cagaagctca
     4621 gcggccagat cccggccttg atcagaaagg cgacccccag aatggcggcg ccggcctcga
     4681 gaagggcgag atcgtgggcg tcggtgctcg cgacgcgcat tgcaagatcg gccaagttga
     4741 gcgtcccggt gaccgagtaa agcatggcaa ccccgagcag gaaaagtagc gaggcggcga
     4801 tgttgatcgt gatatagtgg aggctggcgc tcgtacgctc ggttcccgag ccatgcagga
     4861 tcagcccgta ggacgccgcg agcaggacct cgaaaaacac gaagagattg aagatgtctc
     4921 cggtcaggaa ggcaccgttt acgcccatca gcagcagcag gaacagggcg tgaaagcgag
     4981 gaccggagcg gtcccatcgc gcgatggcat agaatagcgc cgtaaatccg acgacgctgg
     5041 ccaagagaag catcatcgcc gacagccggt cggcgaccag gacgatcccg aagggcgcgg
     5101 cccagttgcc gagcagatag acgagcggac cgctgccatc gcccgcaagc cagagcagcg
     5161 cggcggcgtt gacaaccagg agcgccgcgg tgccgagacc gagcacccgc ttcaaaacgc
     5221 ggcggcgttc gtcgaaggcc agcatcaatg cgctcgccat caatggcagg atgatcggcg
     5281 tgatcaccag atgatggggc caaccggtca tggttcgcca tcctctccgt cgacatggtc
     5341 ggtgcccgtc agtccgcgcg aggcaaggag cacgacgaga agcagcgccg tcgtggcaaa
     5401 ggagatgacg attgcggtga gaacaagcgc ctgtggcaaa gggtcggcgt agcgcgcagg
     5461 atcggccgac gcggggtcgc ggccgacgat cggaggcgcg tcgctgcgca gtcgtcccgc
     5521 cgcgaggatg aagaggttca cggcatagga cagaagcgaa agcccgagaa tcacctggaa
     5581 ggttcgcggt cggaagacca gccacaatcc cgatgccgtc aagacgccga cggctagggc
     5641 aagtacgacc tccatcagac ggagtctccg gctggtgtcg ctggcggcgc agcgatcggc
     5701 ttcttcgccc gcggcgcgcg gatcgactgg tgcgcgatcg cgatgaggat gagcacagtc
     5761 gtcccgacga cgaccgcgaa gaccccgatg tcgaagagga gggcggtcgc gagcggaata
     5821 tccccgatcc acgggatcgt gagatagcgc gaatggctgg tgaggaaggg atagccgaat
     5881 agccaggctg caaggcccgt tccgcccgcg caaagaaggc ccgcgctgat ccagcggacc
     5941 ggctcgatgt ggaggctggc ttcgacggcg cgcgtgccgt tggcgatata aagaagaagg
     6001 atggcgatcg acgctgcgat cccgcccgcg aaaccgccgc ccggcaggtc atggccgcgc
     6061 atcagcagat agatcgcgaa cattaggatt acggggaaga gccactggat aatcgcgcgc
     6121 ggaacgagga gataatgagc aagggtgtcg ccgcgcgcat ggccctcgcg cgaatcgtcg
     6181 aacgcgttct gccggtgctg ctgcatcggc atttcgatgc tttcgggtgc tgggcggaaa
     6241 cggcgaagca gcgtgaacac ggtgagcgcg acgatcgcta gaaccgtgat ctccccaagc
     6301 gtgtcgaagc cccgaaaatc gacgaggatc acgttgacga cattggtgcc gcccccgccc
     6361 ggataggctt gctccacaaa atagcgtgac aggctttcgg gcaggggacg tgtcatcatg
     6421 gcataagcga gcgccgccat cccggtgccc ccaagcagcg cgatgagaag gtcgccgccg
     6481 cgccggacgc cgacggtccc gggcttgctt ccagccggcc aaacatcggg aagccgcagc
     6541 ggcagccagc gcagaccgag aagcagcagg acaagcgtga cgatttccac gagcagctgg
     6601 gtcagcgcga gatcgggggc ggaaagccag atgaaactga tgcaggtggc aaggcccgcg
     6661 ccgccgatca ggacgagcgc aacgagccgg tggaatttcg ccagcaccgc ggctgcgacc
     6721 gcgcacgccc cgcccaatat ccacagcagg gcaaaaaccg gatcgatcag cgtgtcaggg
     6781 acggaaccgc ggctgtaccc atagcgcagg aagggaagcg tgccggcaac gagcgccacc
     6841 acgatgatga ggcgcagctg gacttgcagg cgtttcgtcc cgaaccagcg cagcagtgcg
     6901 cgcgccgcca gaacaatcgc ccccagcacc gcctcgaacg tgcggcggcc cttgagcctg
     6961 ccgatgaacg gcgcccgctc ggtcgcgttc agcctggcgc ggaacaggat gtagagcgcg
     7021 acgcccccca gcagcgcggc gaagctcatc aggagcggaa gcgtaaagcc gtgccagacg
     7081 acgagactgt attcaggcgc gcgcgcgccg agcaccgatc ccaccgccgt cgcaaggaag
     7141 gggccgattg tcagcgcggg aagcacaccg acgagcaggc aggcgagcac cagaatctcg
     7201 attggcagac ggatccacgg cggcgcctcg cgcggctcgt gcgacagtcc cgacggcgcc
     7261 gggccaaaga agatctggtg aatgaaacgc accgaataca ggacgctgaa catgctggcg
     7321 agcgtggcga gataggggag ggcgacgtcg atcagcgtgc cgctatgcgc ctcgagggct
     7381 tcggcgagaa acatctcctt cgacaggaag ccgttgagca gcgggacgcc cgccatggcg
     7441 gcggcggcga ccatcgcgag cgttgccgtg atcggcatga accgcgcgag cccgctgagc
     7501 aggcgcaggt cgcgcgtacc cgtctcgcga tcgatgatgc cgacggccat gaaaagcgag
     7561 gccttgaacg tcgcgtggtt gacggtatgg aaaatggcag caacggcgcc gagcgggctg
     7621 cccaacccaa gcagaagcgt gatgagaccg agatggctga tcgttgaata ggcgagcagc
     7681 cctttcaggt cctgttggaa gatcgccgac caggcgccga ccaacagcgt tgcaagtccg
     7741 gcaccggtga cgatgaggaa ccagctttcg gtgccggcga ggaccggcca cagcaaaatc
     7801 agcaggaaaa tgcccgcctt caccagcgtt gcggagtgaa gataggcaga gaccggcgtc
     7861 ggcgcggcca tcgcatgcgg cagccagaaa tggaatggaa actgtgcgct tttggtgaag
     7921 gcgccgatca ggattaatat gagcgccgga agataggcgg cgtcattctg gatggccgtt
     7981 ctagccgcga gcacgacatc gacgtcatag cttcctgccg cgcgcccgag cagcattagc
     8041 ccgacgagga ggcaaacacc gccggctcca gtgacgatca gcgccatgcg cgcgccgtca
     8101 cgggcgctct ggttgtggtg ccagtagccg atcagaagaa aggaaaagag gccggtcagt
     8161 tcccagaaga aggcgatctg gataagattt cccgacagga gaatgccgag catcgcgccc
     8221 atgaacgcct gcaggaacgc gaaaaagcgc ggtacgggat cggtcgccga catataatag
     8281 cgcgcgtaga agctcacgag cgcgccgatg atgaggacga gcatggcgaa cagccaggtg
     8341 aagccgtcaa gccgcagcgt cagattgagg tccagggccg ggagccagga tatttcaaac
     8401 cgggcaaccc tgccggtgat gacgatggga tacaggctgg cgatcattgc cgcaccggcg
     8461 agcgcgttga atcccgtgag cgaggcggca acatcccggc cgcgctgcgg caggaaggca
     8521 gcgatagtgc tcccgacaaa tggcagaatc aggacgagca agagaagcct tgtatcgtcc
     8581 acctcgactg catctccttc gttccgatta ccaacccccc ggcttatggt ggatagctcc
     8641 ctgacagggc aaggcttagg accgggattc tcatcaaaat cctcttcgct cgaacaattt
     8701 tcccgggcgc atcgatcaag tcggcgcggc ggaccctatg gcaagtaaaa cgaacgggcg
     8761 gggcaatcgg ggagcttggt cgaaccggcg cagcggcggt ggcctcgttt caccgggcca
     8821 tattatcgcc tggaaccgac caggacacgc cgcgccgatc cgatcggatt ccgcctcacg
     8881 caagcccgcg cgagttcaca cgcagttatc ttggcgcggc tcctcgatcg tgccgaaacc
     8941 gaaagcggcc atcacgaaat cggtctccgc caggagtttg tcatggctcg cgccagcctt
     9001 cgcgcgcaag gcgatgccgc aacaatgggt aataagaaag gacgccgcat caccgtcgcc
     9061 gccgggcggc gcggtgccgc ccgcttgaaa cctggcgagg ctggccgcca acttccgttc
     9121 atagacttcg atccgttccg ccagaaggat ccggacgccc tcgtcctcgg gcgaacccgc
     9181 cggagccccg tgcgcaagaa gcgagccgcg cggggtgccg gttccaaagc atgcgcgcac
     9241 cgttccctcc agcagccgcc gaacgatcaa atccggagtc tcgcttgcca gtgcgtcgcc
     9301 aacgaacgcc aggtaggtgc tttcgaaatg ctcgaccacg cggacgaaca aggcctcctt
     9361 gttgccgaat acgcggtaca ggctgggcct tttgataccc atcgcttgcg tgagatcatc
     9421 gagggtcgtg ccggcatagc ccttcgccca gaattctcgg gtcgctcgat caagcgcttc
     9481 gtcgatgtcg aactgccgca tccttcccat gattttcaac ctaccctaac gattcacgtg
     9541 aatgttcggg gacccgctct gacattgtga cggccgacga ccagcgttgc tatcgccgaa
     9601 aagacggcct tgtctgcgag ctgaccggcg tatttaaggc attaccacaa ctgcggtaag
     9661 gatcgccatc ggcaatccga tcgccacgac tgcggcgagc gccaaagtcc aaacattctc
     9721 gctgatcgtc atgagcttac tcctttgaaa ttgcctgcga gccaaagctg cggcctttga
     9781 ggggcgtttt aatcaaaaag ccgccgcagg tggcgctaaa gaagttgagg tcagccgcag
     9841 attctgcgct atgaagtgct gatggcagca gggcgcgcgt taaccctcgc acggacgcgg
     9901 ccggcgcatc gccaccgccg ccgcgacctt agtcgacact tggtcgaagc acgcacctgt
     9961 tgatgtcgga gcgactgcgc ttttactccg gcagcgagta gggcgtttgg tgacggatat
    10021 gtcgctcctc gtgatcaaag gcgatcgacg atgctggaag cgctcgcatc tgacgaattt
    10081 ttgcgcaccg ttgtgctcat tctcggcctc tgcgggacgt tcgcctttgc gctcagcagc
    10141 gcaacggcag ggatcacgcc cacggcgctt gaggcgactt gtcgtactgc gcgaccccat
    10201 cggctatgcg gagggccgta ggtccgctat cctgaaattg cgcccagaag cagacagtct
    10261 gaaatcggcc atttgccgaa agagaaccgc ccgcccgatc gacatcggac gggcgtaaga
    10321 tgaagagccg ggttaattga agggaagagc gctccagagt cgcatggcgc ggctatcgcc
    10381 ttaggcgggt cgctatttcc gccctgcagg cgagggcgtg tgcggctcgg ccgcgttgtc
    10441 ggtcatgcat gcgtcttcgc ccacggaccg gggtggaggt tgcgccgctc gatcgctggc
    10501 gtagatgttc gcagcagggg tttccagatg ggcttccagc aggtcgaaga tggctggatc
    10561 ttcttccgca tcgaacttgc gggcgaaaag gtcaggactc agcgtcagcc gcaacgcgtc
    10621 tgccgtgacg aaggtgcggg gccgcaattt gatgtcgccg tcaggaaccc agtcgatcgt
    10681 gcgcaaatcg tcgttcacga cctcgccaag cccgcggctg ttcatcagca gcgtctggaa
    10741 aaaggcttca tccgggatga aacttcgccg gtagaatgtc ttgaaacgct cggcgctcgg
    10801 atgatggacc gcgaaatggc agaagctgcg cgtcacggcc ttccactggg tgccgatata
    10861 gggcgtcgcg tctttcagga aagcgcgggg gaggtgtgtc cgaaacatgc gtcctatcgc
    10921 ttcgacgaat atatggctga cgcggttcat cgtatcgggc cgcattgtcc gctggtcgag
    10981 agcgcggatg aactggcggc ggggatgggc gcgcaggaag gatcggatat aggcttggga
    11041 cttcagcgga aaatcctgcc cgctgaggtt gatgtagtgc gtccagtccc ggttcatttt
    11101 cagcagccgc ttcattccgc gcagctccgc gtcgacgagg ctgtagccac cccaaagcgc
    11161 ttgcttcgat tcgatcagtt cgacaccttc ataggtcttc aggaaagcag cgatgtctcc
    11221 agcaaaaacg ggtcctgagc ctttgtcgac atggatcaca tagctgtttc cgggggcgta
    11281 gatcgcccga aacatccgtt tgaactgttc gggaaagcgg tgaaggagaa tgaaatagcc
    11341 gatcatgaga gtatgccttt cggatcgtcg atccgggctg tcagctcacc ggaatcgcga
    11401 tccatttcct gtcccggaaa acccaaccaa agtcattttg atcgatagga gacaatgccc
    11461 gacaggtggc cggccgcgtt gcgccgcggc gtggtccaat ggcacaggat tgatatgggg
    11521 atcgcgggcg ccaatgtaag tggccggggc actaacatgc gcttttgcta cctgcctgta
    11581 tggaaatcga accgttcgcg atctgtcggt gatcgtcagc accccgacct attagcccag
    11641 ccgtcctatc gcctcggaac ttgtgtcacc cggcttcgtt gcctcgtcac cgtcgtcacg
    11701 catgggcaag cgatggctga gagacatgtt gcgctcccgc tcgaaggagg cgcgcgcgat
    11761 gttcaattcc cggagaattt tgattgtaga ggacgagccg ttcgtcgtga tcgaactcgt
    11821 tgaggcggtc gagcgatcgg gaggcgcggt cgtcggcccc gttccaacag ttgccgaagc
    11881 gctggcgctg ctcgatcagc acaagatcga cgcggccatc ctcgacgtcc agttgctcga
    11941 cggcgaaatc tcgcccgttg ctctccggct catcgagcag ggcacaccgc tcgtatttca
    12001 gaccggaacg ggtcttccgg tcgtgctcgt cgaactcgat attgagttgg ccgtcgtcct
    12061 gaagcccgtc gctccgatga tcatactcga attgctatta gccgagatca tgaggcaaac
    12121 cgcccggcag gtcggttagc gaggtacaag cgctatcacg gcgtcagatt tgcatagccg
    12181 catcggacgc gccatgctcg cgcggatttc gtggtcagcc attatctttc agcttggcgg
    12241 tcaacttcac gggggaagcc tccttggctg cgagcgcggt cgtcggtttg cccttggcgg
    12301 ctttgggctt gcggatctcg cgattgctgc gcttttgcga tttggccatg atggtttctc
    12361 tctttttgcg gttgctggat gggaagacgt gaggcggcag gtcgcgctgg gaaatgcttt
    12421 caggcgccgg ctagtggctt ctgcgcgaag gagtgcgcgg acgcgtcaat tttcgttcgc
    12481 ggacggacga tagaagcggt ggcgcacgac gctgccttcg atcaggtggc cgcgaagccg
    12541 cagtcgcatc tcctcggcgc ccgcactcga tgtgcagacg aggcgcgtgc cgccggactg
    12601 aagcggctcg atcgcgctga tgctgacgcc ctgcgccagg cagaggcgcc tgacctcatc
    12661 ttcgggcagg gacaggttca tggcccggct cattcggacc gccgcttgct tttccacgcc
    12721 cgtatagcgg ccaccacatg ctcataacct tcggcactgg cggtcgccag cgcgatcggg
    12781 cggcctccca gttcctcatt ctcggcgttt agaaacgcga ccgctgcgtc gcggccaccc
    12841 atgactaagt aagcgagctg actgatatcg ccttgccgcc gtgcatcctc aagtgcgaag
    12901 ggcacccgac tcttgcgaaa aaagctgttc gaccgccctt tggtcggcgt cggctcggcc
    12961 gggcttggag cctcggcgtt ctgtatgcgg gaagtcatga cgcggctcct tcgtaagcgg
    13021 gagcgcgatg tctctcagcc gcgacggcct acaatgtccg gtgccgcgat cttatgaata
    13081 tgggggcaat gcccgaagat gtaagatgcg atcggtccgc tgtccgcttt tcttcatctg
    13141 gtccccgaag cggtctgact gcaaacggcc aagacgggtt ctgcgccgtg gcgcggcggt
    13201 gataacttta tcatccgtcg tgcctatgga acgctaagac ggcatgctcg caaggtcttc
    13261 aaggaccgcg gcccaataag cgatttcctg accgttgccg cgttcacgag cccactcgag
    13321 ttgccttgcc acgagctgct tggcctcgtt tccgtgtcgt agcttcagtt gggctgagca
    13381 gccgcgaacg ttttgactgc cgtaaaaggg catgacagtt ccctccgcat tctccttggt
    13441 tgcgaacatg aaggggatag ggcggcggtc gcccgccgcc ct
//
LOCUS       DATMSU010000510         7092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_131985,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000510 DATMSU010000000
VERSION     DATMSU010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131985"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..742
                     /locus_tag="VN034_13525"
     CDS             62..742
                     /locus_tag="VN034_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWV61677.1"
                     /translation="MKVSHVCEADHDIVTAAVAAAEAQTSGEIVTVVAGKSNDYDDVA
                     LVWASIIAFLAMSIVALFPDFYRGLYDQLTGGWGHELTANQWLGTVIAIGVVKWIGMW
                     LILLWRPLRMALTPRSIKAERVRARAIDLFKVGTEGKTTGRTGVLLYLSLKEHRADIV
                     ADEAIAAKVTPDVWGDAMAALIERVRAGKPGEGMAEAVRQMGILLAQHFPRGSENPNE
                     LPDRLIEL"
     gene            754..1299
                     /locus_tag="VN034_13530"
     CDS             754..1299
                     /locus_tag="VN034_13530"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWV61678.1"
                     /translation="MSRPGPDTPIETRWEGRFITVKQQGTWEYVARSRGIHAAVILAI
                     DEGDDGRHVILVEQYRVPLKINCLELPAGLVGDDMAGEASEIAAERELEEETGYRAAR
                     WRTVGEFYSSPGMVSESFTLLIATGLTKVGEGGGVDGEDIVVHRVPLAGIEDFIATKR
                     AEGCGIDVRVAMLLAGGLLGV"
     gene            complement(1565..2212)
                     /locus_tag="VN034_13535"
     CDS             complement(1565..2212)
                     /locus_tag="VN034_13535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWV61679.1"
                     /translation="MKPIDAPVTIGFALTCIILFAFLSVTGLVGHVGMAAGMVPARLG
                     TELFVFDGATVPAMLTPITAAFLHHDALHLLFNMVFLIYIGRQVELPLGAKATILLMV
                     GGAYAAALATWASDPGSTAIGVGASGVVSALIACYALIFNRQQVRALGPIPGIWVRIL
                     WLAAAWIGLQLLIGFASNMAPSNDRVVPGIWAHIGGFLAGLLLTRPLLRWRFGAR"
     gene            complement(2226..2702)
                     /gene="greA"
                     /locus_tag="VN034_13540"
     CDS             complement(2226..2702)
                     /gene="greA"
                     /locus_tag="VN034_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWV61680.1"
                     /translation="MASVEKVPMLAEGYEKLTAQLSALKAERPLIVDAIEEARAHGDL
                     SENAEYHAAKERQGQVEATIGDLEDRLSRAQVIDPTTLSGDRIVFGATVTLADEDDKP
                     VKYQIVGQAEADAKDGKISYNSPLGRALIGRRVDDEVEVTVPSGDKYYLVTKIEFI"
     gene            complement(2798..6118)
                     /gene="carB"
                     /locus_tag="VN034_13545"
     CDS             complement(2798..6118)
                     /gene="carB"
                     /locus_tag="VN034_13545"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWV61681.1"
                     /translation="MPKRTDIQSILVIGAGPIVIGQACEFDYSGTQAIKALKEEGYRI
                     VLVNSNPATIMTDPDLADATYVEPITPEIVAKIIAKERPDAVLPTMGGQTALNTALAL
                     AQDGTLEKYGVEMIGADAEAIDKAEDRQKFRDAMDKIGLESARSGVAHTLDEAFAILE
                     RTGLPSIIRPSFTLGGTGGGIAYNREEFEHIVRGGLIASPTTEVLIEESLLGWKEYEM
                     EVVRDRKDNCIIICSIENVDPMGVHTGDSITVAPALTLTDKEYQIMRNASIAVLREIG
                     VETGGSNVQFAVNPKDGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     IMNDITGVTPASFEPTIDYVVTKIPRFAFEKFKGAEATLSTAMKSVGEVMAIGRTIHE
                     SLQKALRGLETGLSGFNFVERLKGATHDQLRNELAQRTPDRLLNAAQAIREGLPLEEI
                     NRVAGYDMWFLERIAEIVAAEVDVCTNGLPRDAEGLRKLKAMGFSDKRLAYLALQSAN
                     LHPGTRRATARGSGLIHEAVKAMTGGVTEAEVRALRHKLGVRPVFKTIDTCAAEFQAQ
                     TPYLYSTYEAPTFGEPENEANPSDRKKIVILGGGPNRIGQGIEFDYCCCHACFALEEA
                     GYETIMINCNPETVSTDYDTSDRLYFEPLTAEDVLEILHVEMSKGELVGVIVQFGGQT
                     PLKLAQALAEAGIPILGTSPDAIDLAEDRERFAALVNKLGLKQPENGIARSREEAVAV
                     AARIGYPVLTRPSYVLGGRAMEIVDDQAQLENYIETAVQVSGDSPVLIDRYLRDAIEV
                     DVDALCDGTDVVVAGVLQHIEEAGVHSGDSACSIPPYSLSAGIVAEIERQTEALARAL
                     EVRGLMNIQFAVKDDEVYLIEVNPRASRTVPFVAKAVGSPIAKIAARVMAGEKLADLP
                     KINRDIKHVAVKEAVFPFARFPGTDPVLSPEMKSTGEVMGIDADFNLAFAKAQLGAGD
                     RLPTTGRLFVSVKDSDKPRIIGAVKQLADWGWKVIATGGTADYLAGEGIDVERVNKVA
                     EGRPHIVDRIKDGDVQLIFNTTEGWQSLQDSQSIRASALAADIAYYTTAAASDAATQA
                     IGALRSRSLEVKPLQDYY"
     gene            complement(6118..6414)
                     /locus_tag="VN034_13550"
     CDS             complement(6118..6414)
                     /locus_tag="VN034_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61682.1"
                     /translation="MIGRAVLTIAALALFGCAAAPEDGEGMASALARVEKQCGVAPGM
                     LTLVADGSVQVQPSPYEKYERVDCVLNELRKPEFADHVKLGFVGNEAYGKEEKE"
     gene            complement(6411..>7092)
                     /locus_tag="VN034_13555"
     CDS             complement(6411..>7092)
                     /locus_tag="VN034_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HWV61683.1"
                     /translation="GWAGLEGMDLAKAVSRTDTGDWTGGAWTLGHGYATADGGERPHV
                     VAIDYGAKDNIFRNLVKAGARVTVVPATASLDEVLGHKPAGVFLSNGPGDPAATGDYA
                     VPVIQGLLERDVPIFGICLGHQMLGIAAGAKTVKMHQGHRGANHPVQRAEDGVVEITS
                     MNHGFAVDASTLPAGVVETHKSLFDGSNCGIAITGKNAFSVQYHPEASPGPQDSFYLF
                     EKFVGGLK"
BASE COUNT         1195 a         2328 c         2268 g         1301 t
ORIGIN      
        1 gggcggcggc ggctctttcg ggggcggcgg cgcctcgggc ggctggtaag ggagcaaggc
       61 catgaaagtc agccacgtct gcgaagccga tcacgacatc gtcaccgccg cggtcgccgc
      121 ggccgaggcg cagacgagcg gcgagatcgt taccgtcgtc gcgggaaaat ccaatgacta
      181 tgacgatgtc gcgctcgtct gggcgagcat tatcgccttc ctcgccatgt cgatcgtcgc
      241 gctttttccc gacttctatc gcggcctcta cgatcagctg accggcggct gggggcatga
      301 actgacggcc aatcaatggc ttggcacggt gattgcgatc ggcgtcgtca aatggattgg
      361 catgtggctg attctgctgt ggcgtccgct gcgcatggcg ctgaccccgc gcagtatcaa
      421 ggccgagcgg gtgcgcgccc gcgcgatcga cctgttcaag gtggggaccg aaggaaagac
      481 caccggccgc accggggtgc tcctctattt gagcctcaag gagcatcgcg ccgacatcgt
      541 cgccgacgag gcgatcgccg cgaaggtgac gcccgacgtg tggggcgatg cgatggcggc
      601 gctgatcgag cgcgtgcgcg ccggcaagcc gggcgaaggc atggccgagg cggtgcggca
      661 gatgggcatc ctgctcgcgc agcatttccc gcgcggcagc gagaatccga acgaactccc
      721 cgaccgcctg atcgaactct aaaaggaaat atcatgagcc gccccggccc cgacaccccc
      781 atcgaaacgc ggtgggaagg ccgcttcatc acggtcaaac agcaggggac gtgggaatat
      841 gtcgcgcgct cgcgcggcat tcacgccgcg gtgatcctgg cgatcgacga gggcgacgac
      901 gggcggcatg tcatcctggt cgagcaatat cgcgtgccgc tgaagatcaa ctgcctcgaa
      961 ctgcccgcgg ggctcgtcgg cgacgacatg gccggcgaag cgtccgagat cgcggcggag
     1021 cgcgagcttg aagaggaaac aggctatcgc gccgcacggt ggcgcaccgt cggcgaattc
     1081 tacagctcac ccggcatggt cagcgaaagc ttcactctgc tgatcgcgac cggcctgacc
     1141 aaggtcggcg agggcggcgg ggtcgacggc gaggacatcg tcgtccaccg cgttccgctc
     1201 gcgggcatcg aggatttcat tgcgacgaag cgcgccgagg gctgtgggat cgacgtacgc
     1261 gtcgcgatgc tgctggcggg cggattgctt ggcgtatagc tggtttcggc cggtagcgaa
     1321 cattgagaaa tgtgcatcag ccccacctct tcagaggagg ggtagcgaga cttacgaact
     1381 tgttcgttag tcgcagcggg gtggtgcttg cggggaagcg tgcgcctccc gccctcacca
     1441 ccccgttcga ctaaaggccc ggctgcgccg gaccaagtct cactacccct cctctgaaga
     1501 ggaggggttt ggggcatcgt ccactcccca ccccaaatcc gacaccatcc tcggaagcgg
     1561 gtgcctaccg cgccccgaag cgccagcgca gtagcggacg ggtcagcagc aagccggcaa
     1621 gaaaaccgcc gatatgcgcc cagatgccgg gaacgacgcg gtcgtttgac ggcgccatat
     1681 tgctcgcgaa cccgatcagc agctgcaatc cgatccaagc agccgcgagc cacaatatgc
     1741 gtacccatat ccccgggatc ggtcccagtg cacgcacctg ctgtcgattg aaaatcaggg
     1801 catagcaggc aatcagcgcc gagacgacgc cgctggcgcc gacgccgatc gccgtcgagc
     1861 cgggatccga tgcccatgtg gccaaggcgg cggcataagc gccgccgacc atcagaagga
     1921 tcgtcgcttt cgcccccagc ggcaattcaa cctgtcgccc gatgtagatc aggaacacca
     1981 tgttgaagag cagatgcagc gcgtcgtggt gcaaaaacgc cgcggttatc ggcgtcagca
     2041 tcgcgggaac cgtcgcgccg tcgaacacaa acaattctgt gcccaagcgc gcgggcacca
     2101 tgcctgcggc catgcccacg tgcccgacca atccggtcac cgacagaaag gcgaacagga
     2161 tgatgcaagt cagcgcaaag ccgatcgtca ccggtgcatc gatcggcttc accctcgctc
     2221 gcccgtcaga tgaattcgat cttggtcacc agataatatt tgtcgcccga aggcacggtg
     2281 acctcgacct cgtcgtcgac gcggcggccg atcagcgcgc ggccgagcgg cgaattatag
     2341 ctgatcttgc cgtccttggc gtcggcttcg gcctgcccga cgatctggta tttgaccggc
     2401 ttgtcgtcct cgtcggcgag cgtgacggtc gcgccgaaca cgatgcggtc gcccgacagc
     2461 gtcgtcgggt cgatcacctg cgcgcgcgac aatcggtctt cgaggtcacc gatcgtcgct
     2521 tcgacctggc cctggcgctc cttggcggcg tgatattcgg cattttccga aaggtcgcca
     2581 tgcgcgcgcg cttcctcgat cgcatcgacg atcagcggcc gctcggcttt gagcgccgac
     2641 agctgcgcgg tcagcttttc atagccctct gcgagcatcg gcaccttttc aacgcttgcc
     2701 attatcctgt tgtccttcgt caataatcca gcgccagcga aacttcgccg ggcggctgcg
     2761 ccgcttttcc gtcaatgtcg gggggtggag cggtccttca ataatagtcc tgaagcggct
     2821 ttacttcaag agagcgcgag cggagcgcgc cgatcgcctg cgtcgccgca tcgcttgccg
     2881 ccgccgtcgt ataataggcg atgtcggccg ccagcgccga cgcgcggatc gattgcgaat
     2941 cctgcagcga ttgccagcct tcggtcgtgt tgaagatcag ctggacgtcg ccgtccttga
     3001 tccggtcgac gatgtgcgga cgcccctcgg cgaccttgtt gacgcgctcg acgtcgatcc
     3061 cctcgcccgc cagatagtcg gcggtgccgc cggtcgcgat gaccttccag ccccagtcgg
     3121 cgagctgctt caccgccccg atgatgcgcg gcttgtcgct gtccttgacc gagacgaaga
     3181 gccgcccggt ggtcggcagc cggtcgcctg cgccgagctg cgccttcgca aaggcgaggt
     3241 tgaaatcggc atcgattccc atgacttcgc cggtggactt catctcgggg ctcagcacgg
     3301 ggtcggtgcc ggggaagcgc gcgaagggaa agaccgcttc cttcaccgcg acatgtttga
     3361 tgtcgcggtt gatcttcggc aggtcggcga gcttttcgcc cgccatcacc cgcgccgcga
     3421 tcttggcgat cggcgaaccc accgccttgg caacgaaggg cacggtgcgg ctcgcgcgcg
     3481 ggttgacctc gatcagatag acctcgtcgt ccttgaccgc gaactggata ttcatcaggc
     3541 cgcgaacttc gagcgcgcgc gcgagagctt ccgtctggcg ctcgatctcg gcgacgatgc
     3601 cggccgacag gctgtacggc gggatcgagc aggcgctgtc gcccgaatgg acgccggctt
     3661 cctcgatatg ctggagcaca cccgcgacga ccacatcggt accgtcgcac agcgcatcga
     3721 cgtcgacctc gatcgcgtcg cgcaaatagc ggtcgatcag caccggcgag tcgcccgaca
     3781 cctgtacggc ggtctcgata taattttcga gctgcgcctg atcgtcgacg atctccatcg
     3841 cacggccgcc gagcacatag gaggggcgcg tgagcaccgg gtaaccgatg cgcgccgcga
     3901 ccgcgacggc ttcctcgcgg ctgcgcgcga tgccattctc gggctgcttc aggccaagct
     3961 tgttgacgag cgcggcgaaa cgctcgcggt cttcggcgag gtcgatcgcg tcgggcgagg
     4021 tgccgagaat cgggatgccc gcctccgcca gcgcctgcgc gagcttcagc ggcgtctgcc
     4081 ccccgaactg cacgatcacg ccgacgagtt cgcccttcga catttcgacg tgcaggattt
     4141 cgagcacatc ctcggcggtc agcggttcga aatagaggcg gtccgacgtg tcatagtcgg
     4201 tcgacaccgt ctcggggttg cagttgatca tgatcgtttc atagcccgcc tcttcgagcg
     4261 cgaagcaggc gtggcagcag caataatcga actcgatccc ctgcccgatc cggttcggac
     4321 cgccgccgag gatgacgatc ttcttgcgat ccgacgggtt cgcttcattc tcgggctcgc
     4381 cgaaggtcgg ggcttcgtag gtcgaataga gatagggcgt ctgcgcctgg aattcggcgg
     4441 cgcaggtgtc gatcgtcttg aacaccggcc gcacaccgag cttgtggcgc agcgcgcgga
     4501 cttcggcctc ggtcaccccg ccggtcatcg ccttcaccgc ctcgtggatc agcccgctgc
     4561 cgcgcgcggt cgcgcggcgc gtgccggggt ggaggttcgc cgactggagc gcgagatagg
     4621 cgagccgctt gtcggagaag cccatggcct tgagcttgcg caagccctcg gcatcgcgcg
     4681 gcaggccgtt ggtgcatacg tcgacctcgg ccgcgacgat ctcggcgatg cgttcgagga
     4741 accacatgtc gtaacccgcg acgcggttga tctcttcgag cggcagcccc tcgcggatcg
     4801 cctgcgcggc gttgagcagc cggtcggggg tgcgctgcgc caattcgttg cgcagctgat
     4861 cgtgcgtcgc gcccttcagc cgctcgacga agttgaaacc gctgaggccc gtctccagcc
     4921 cgcgcagcgc cttctgcaag ctttcgtgga tcgtgcggcc gatcgccatc acctcgccga
     4981 ccgatttcat cgcggtcgac agcgtcgcct cggcgccctt gaacttttcg aaggcgaagc
     5041 gcgggatctt ggtgacgaca taatcgatcg tcggctcgaa cgacgccggg gtgaccccgg
     5101 tgatgtcgtt catgatctcg tcgagcgtat agccgaccgc gagcttcgcc gcgaccttgg
     5161 cgatcgggaa gcccgtcgcc ttcgacgcga gcgccgacga acgcgacacg cgcgggttca
     5221 tctcgatcac gatcaggcgg ccgtccttgg gattgaccgc gaactggacg ttcgacccgc
     5281 ccgtttcgac gccgatctcg cgcagcaccg cgatgctcgc gttgcgcatg atctgatatt
     5341 ctttgtcggt cagcgtcagt gccggcgcga cggtgatgct gtcgcccgtg tggacgccca
     5401 tcggatcgac attctcgatc gaacagatga tgatgcaatt gtccttgcgg tcgcgcacca
     5461 cctccatctc atattctttc cagccgagga gcgattcctc gatcaggact tcggtggtcg
     5521 gcgaggcgat caggccgccg cggacgatat gctcgaattc ctcgcggtta tacgcgatgc
     5581 cgccgccggt gccgcccaag gtgaacgatg gacggatgat cgagggcaag ccggtgcgtt
     5641 cgaggatcgc gaacgcctcg tcgagcgtat gcgcgacgcc agagcgcgcg ctttcgagcc
     5701 cgatcttgtc catcgcgtcg cggaacttct gccggtcctc ggccttgtcg atcgcctcgg
     5761 cgtcggcgcc gatcatctcg acgccatatt tctcgagcgt gccgtcctgc gcgagcgcga
     5821 gcgcggtgtt gagcgcggtc tgcccgccca tcgtcggcag caccgcgtcg gggcgctcct
     5881 tggcgatgat cttcgcgacg atctcgggcg tgatcggctc cacataagtg gcgtcggcga
     5941 ggtcgggatc ggtcatgatc gtcgccgggt tggagttgac gaggacgatg cgatagccct
     6001 cctccttcag cgccttgatc gcctgcgtcc ccgaatagtc gaactcgcat gcctgaccga
     6061 taacgatcgg gccggcgccg atgacgagga tggattggat gtcggttctt ttgggcatta
     6121 ttctttttct tcttttccgt atgcttcgtt acccacgaag cccaatttca cgtgatcggc
     6181 aaactcgggt ttcctgagtt cgttcaggac gcaatctacc cgttcgtatt tttcgtaagg
     6241 cgatggctgc acctgcaccg atccgtcagc aacaagcgtc agcatcccgg gcgccacgcc
     6301 acattgtttt tcgacacgcg ccaacgcaga ggccattcct tctccatctt caggagcggc
     6361 ggcgcagcca aacagggcca aagcagcaat ggtcagaact gcacggccga tcacttcagc
     6421 ccacccacaa acttctcgaa caggtaaaag ctgtcctgcg gccccgggct cgcctcgggg
     6481 tgatattgca cgctgaacgc gttcttgccc gtgatcgcga tgccgcagtt cgatccgtcg
     6541 aacaggctct tgtgcgtctc gaccacgccg gcgggtagcg tcgacgcgtc aaccgcgaag
     6601 ccgtggttca tgctggtgat ctcgaccacg ccatcctcgg cacgctgcac cggatggttc
     6661 gcgccgcggt ggccctgatg cattttcacc gtcttcgcgc ccgccgcgat gccgagcatc
     6721 tggtggccga ggcagatgcc gaagatcggc acgtcgcgct cgagcaggcc ctgaattacc
     6781 ggcaccgcat agtcgcccgt cgccgcggga tcgccggggc cgttcgagag gaacacgccc
     6841 gcgggcttgt gccccagcac ctcgtcgagg ctcgcggtcg cggggaccac ggtgacgcgc
     6901 gcgccggcct tcacgaggtt gcggaagata ttgtccttcg cgccatagtc gatcgcgacg
     6961 acatgcggcc gctcgccgcc gtccgcggtc gcatagccat gcccgagcgt ccacgcgccg
     7021 ccggtccagt cgccggtgtc ggtgcggctg accgccttcg caaggtccat accttcgagc
     7081 cccgcccagc cg
//
LOCUS       DATMSU010000511        34144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_56144, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000511 DATMSU010000000
VERSION     DATMSU010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 34144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34144
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56144"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VN034_13560"
     CDS             complement(<1..972)
                     /locus_tag="VN034_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWV61684.1"
                     /translation="MTEQAKTAEKRAAAIVDAMTLDEQISLTKSMSGGSLLDLGIPLP
                     PFIPEAMRQPKPDGAIGTAGYVPAIGRVNFPALHLGDASLGVADIGYLRPGQESTALP
                     ATLSLAATFDPAMAREAGRLIGAEAFAKGINVMLAGGVNLARELRNGRNFEYLGEDPL
                     LAGILVGEQIAGIQDRHVASTIKHFTLNPQESGRFVYDARIGEAALRESDLLAFEIGI
                     KRGKPASIMCAYNKVGGTYACENEFLMRDVLKGDWQYSGWVMSDWGAVHSLKPSIDAG
                     LDQQSPQDKDYFAGLPAAVKAGELAPARVRDMALRIVRSMALVGALDH"
     gene            complement(1066..1698)
                     /locus_tag="VN034_13565"
     CDS             complement(1066..1698)
                     /locus_tag="VN034_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61685.1"
                     /translation="MAEKPQRRTQAERSAGTREVLLAAAIEALHEHGYAATSTILVAE
                     KAGVSRGAMLHQFRTKADLMTFVVEAVFEAELQHYSENLRDVTDPRERFLAYPEMAWE
                     VLSRPSGVAVLEILQGSRSDPQLCEMLAPVQARIEKEALEFTHLAAAEDARGAMAVMR
                     LVVWAIRGLSIAQVLAPAPGSVRDSVKVLKRLMAAGLETGMLSVGPEKIK"
     gene            1781..2677
                     /locus_tag="VN034_13570"
     CDS             1781..2677
                     /locus_tag="VN034_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWV61686.1"
                     /translation="MIERRQFLAAAAATAMLPKRAIAAPAKPIIGFQLYMARELLAKD
                     FEATLTAIAATGVRHVEFAGFYDRDAAAIRKTLADNGLIAIGAHAVRADMDDAEIDRL
                     IEQCAAIGMEYVVAPVPLVPAYRALVGKSDNPFRDAIAALTRDDFLRTADRFNAIGGK
                     VQNAGMRFAYHTHGLDFLRFDGRYAFDDMIDRCDPALVAFELDIGNTIAAGVDPLPYL
                     ERLGRRAALAHLKDWQGPIEPAVDRVPPTAPIGEGIVDLKATMRAMTAAGVRYAFIEE
                     EETPAGRVIAAIGSAYRHLSRL"
     gene            complement(2672..3526)
                     /locus_tag="VN034_13575"
     CDS             complement(2672..3526)
                     /locus_tag="VN034_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWV61687.1"
                     /translation="MKSLIFLPLALVSLPVAAGEPAPRVGVVADPCAALPPTPAVVAD
                     YMVRAKAALAAGDPLPPRSEDGMAIYARWQQDLLLADFAGLCRYAAANRALAPATSDR
                     IVFIGDSITEAWLARQPGFFTGDRIDRGISGQTTTQMLARFRTDVIDLKPRVVHIMAG
                     TNDIAGNLGATSLDTIEGNIRTMVDLAEAHGIAVVIGSVLPARQFNWRPEIAPIEPIA
                     ALNARLRALAAERKLVFADYYAALDDGGHGLAKVHAEDGVHPTAAGYAVMRPIAERAL
                     ASAAGKAS"
     gene            3660..5930
                     /locus_tag="VN034_13580"
     CDS             3660..5930
                     /locus_tag="VN034_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61688.1"
                     /translation="MVRFGARRILLGSVTAMALPAMPVFAQDVEPGAATTGDEIIVTA
                     TRREERLQDVPLSVTAVSGESLAQSGLKEVSDIQYLAPNITFSATNPVSNGGGYQIRG
                     VGTQTYDSGVEQTVGLVIDGVVIGLSRDPGSTGFADIERIEVLRGPQGTLFGKNSSAG
                     VIQVVTKRPRMDDASFSLDLKYGERNDRVVQASANVPLGETLALRISGYSNGQDGAIP
                     YVLTPSRAVGDRRNNGVRAKLLWEPSDNLSFLLSGEYQTAFARDGQIIQSLGTSALFN
                     SQFARFAEKPGPNVYKSYMDGDWTADTKLYAGSIEINAGLGDHMLTSITAYRKLETLQ
                     LSDIDASPANIFNNSDGGVDSNQFTQELRLTSPGGSRLEYVLGLYYYRTENGGYAVQY
                     GNYYGLFGAPVVVGGGQRDATNKVRSLAAFGNVTFALTDAFKLIGGLRYTNDRNHGTL
                     VVSPLPFPAVPLGTLPNYDGTVKADNVSGKVGVQFEPSRDFMLYATYSTGFKGPAIDG
                     TAGIIREVRPETVKSWEVGLKSSFLDGKGTFNIAAYWSNYRDFQAQTFDITTTPPTFY
                     LSNAGLLRARGIEVETGFRVTPALRLSASGSYNDATFREYDGQCYPGQPISPVVGQGC
                     YVVPGTTTTVANYRGFRLPNAPKWSYILRADYKQAIGGDMAIDAAANWAWRTKTQAVI
                     GDPNAEIPSYGLLNGTIGFGAEDGSWRFGIYARNLLDKRFYAPYAAGVINPGGYSKIV
                     MPEAFRTIGGTLSFRI"
     gene            complement(6342..7463)
                     /locus_tag="VN034_13585"
     CDS             complement(6342..7463)
                     /locus_tag="VN034_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HWV61689.1"
                     /translation="MPQSALFTPFAVGSLALRNRIVIAPMCQYSAVNGCMTDWHLIHL
                     GNLALSGAALLTIEATAVLPEGRISYADVGLWDDATEAAMARVLDSVRRWSDMPIAIQ
                     LGHAGRKASTEVPWKGGAQLGPDDAGGWRTEGASPFGFAEGDIAPIELDRDGLVRVRD
                     AFAAAAVRAARLGIDAIQLHAAHGYLLHQFLSPLSNRRTDEFGGNLENRMRFPLEVFD
                     AVRNIFPAERAVTVRVSGTDWVEGGWTVEQTIEFAQALETRGCGAIHVSSGGLDPRQN
                     IPVSPGYQVPLARAVKQAVSMPVVAVGLITDYDQAEAVVAGGDADLVALARGILFDPR
                     WPWHAAAHLGAKVEVPPQYLRSQPRKYHELFHVRKLSSR"
     gene            complement(7495..8277)
                     /locus_tag="VN034_13590"
     CDS             complement(7495..8277)
                     /locus_tag="VN034_13590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61690.1"
                     /translation="MQQKLGGKVALVTGGTSGIGAATVARLVRDGAKVVFTGSNAEAA
                     AAVEGETGAIFRAHRVEDASAWDALMAEIEAEYGRLDIAFANAGTEAGDASVEDIGID
                     AWNHIVDVNQTGVMLTVQHAIRAMAKNPQGPKGSIIVNSSMNAHRAMGNYAAYSVTKA
                     AVVALVKSAAIHCAGKGYQVRVNAILPGVVETDMIRGVIDRAPDPAAARAAFEGMAPM
                     KRMAQLGEVSALVAFLASDEAGFISGADYVIDGATTAGMMGV"
     gene            8403..9164
                     /locus_tag="VN034_13595"
     CDS             8403..9164
                     /locus_tag="VN034_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWV61691.1"
                     /translation="MSDSEHPPRIDCVLICGGVWHDMDFARLELLKLLAEDQRVRTRV
                     FENYENIEAIEAADILITYTCDVTPSLPAQEALKCWLAKGGRWYALHGTNSILRLLDT
                     GVWDAPRWAPLMMDLLGSQFISHPAIEPYTVRVADPDHALTKGIEPFETTDELYHLET
                     HGHLHVLLDTECTGLGTGFVEAEDAPGTYPVFYIKQHGAGAVLYLTLGHCRGHYDLQP
                     MMDWWPTVDRCAWDLPVFYDLLRRGIGWLKERESA"
     gene            complement(9287..10924)
                     /locus_tag="VN034_13600"
     CDS             complement(9287..10924)
                     /locus_tag="VN034_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWV61692.1"
                     /translation="MSAGWDYIIVGAGSAGCVLAEQLSADPQVRVLLLEAGGSNDSRW
                     VAIPKGVAKLVTNPEHIWAYGVSQPRGDGAAPSEVWIRGKGLGGSSAVNGMIWSRGEP
                     ADYDEWQKLGCTGWNGEAMTDAFRRLEDHGLGASAMRGTGGPVGVAPKIHSYPLAETM
                     VQAGVEMGLKPVDDLNAATSDRVGFYSHNVRRGRRESAAATFLARAKKRPNLHVVTHA
                     MAERLVVEGGRVTGVEAKVAGQPQQFDCDGEVIVSGGTLESPLLLQRSGIGPADVLRA
                     AGVEPIVDAPDVGGRMREHLGFSMPHRLSRDIGSNRSFYGIGLARAMAQYWLTRGGIM
                     ATGPFEVGAFLNLTSRDMRPDLQLYLGGYTFALGDEKHPVPLNSIDRKPGMTIYGQLL
                     RLTSEGSILIAGPGSDAAPDIKPNWLSTRDDERTAIAAVHAMRRYMAQPALVGLVERE
                     LLPGAAVQSDAEILAAFRRLATCGLHGVGTCRMGSDNRAVTDARLRVKGVDGLRVVDC
                     SVMPSPVTGNTNAPAMALALRAADLIREDRRKLRAVA"
     gene            11052..11801
                     /locus_tag="VN034_13605"
     CDS             11052..11801
                     /locus_tag="VN034_13605"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWV61693.1"
                     /translation="MAGASGRVAGKTAFVTGGASGLGLDIVRRFVAEGAAVVIADIDV
                     GAGEALAAELGQRFVRLDVSNEAEWLAALDACERIDILVNNAGITTHGSIEDLTLEAF
                     RHEFSVDVDGVFLGCKHIIPKMKDRGGSVINMSSMCGVRAQGDLAAYNAAKAAVTHLT
                     KSVALHYARKGYGIRCNSVHPGAIHTPILDKVMAQVADGQALYDSWVATHPVGRLGKP
                     EEISAIVLYLASDESAFATGAEFRVDGGSSL"
     gene            11801..12514
                     /locus_tag="VN034_13610"
     CDS             11801..12514
                     /locus_tag="VN034_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61694.1"
                     /translation="MAGRLDGRAALVTGAGSGIGLATARRLAADGARVLTTDRAGEVD
                     ITADVTAPGINAKLVAEAEARLGGLDIVVACAGITGMQMLDGHDDEFFDAMMTVNVTA
                     VFRLMRDALPLLKRSPHGRIILIGSVMSSFGEAGMAAYSASKHAVLGMTKSVAAEVGA
                     FGITVNCIQPGAIDTPMTAPAFAAMPEFRQRWIDKAALGRLGQPEDIADVAAFLASDE
                     ARFMSGHGVFVDGGATQRQ"
     gene            12645..15038
                     /locus_tag="VN034_13615"
     CDS             12645..15038
                     /locus_tag="VN034_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61695.1"
                     /translation="MITHARMLPAVYATVSLAALAIATPAAAQESPVEESSGGAIREI
                     VVTAQKREESIQSIPIAVTALDEKALESATIDDIRDIAGRVPSLVVDSVGAGPSAAAI
                     AIRGISFEDIEKSFDPAVGVVVDGVFIGTNTGQLLDSFDLERLEVLRGPQGTLFGRNT
                     IGGVINVIRTRPTEDFGVRGQFAYSRFDTKRARLVVNTGKLGDFIALKAFGYYDKTDG
                     FYYNVTKDRREGKYETLTGGVTALITPSDAITAQITYQHTRERGETVASALSETGRDV
                     ICAAPGAPGFSPAEECNRWSLPAHGLYTTFQNIETPVRNNVDSVTGNIDIDLSDNLTL
                     SSVTGYITNRESVTQDFDGSSADFFDTRRQQKYEQFSQELRLLADFDTVNVLLGGYYF
                     DSNYTLDQSTNFGVVLGGGSTAVLRQYVDHSAKSYAGFADAQISLTDAFKISVGARYT
                     KDKKAIFNNYGRIGALVGITLPTFDGTSCVAVTGTTSPAPGVVIPVYSPADNCSGSDS
                     FDKFTWRANAEYTIEPGKLVYASFSRGFRSGGFNGRAASPTSLGPYQPETVDAYEVGL
                     KADWLDRTLRTNLAFYYTKYDNKQEEVVQPSPPGSSSPQETVVRNASSADIKGFEAEI
                     IAQMTDAFSFNASFSYTDAKYKNFFNDIVGLTPGSPADGIADDVSTLTLRRAPKFQWS
                     AGLNYSKEIGSGRFDASTLLRYQSKYVTCIAPNRPVVPSAVVNDNRCFTEDRENLSAQ
                     IGYTFLLGDDREVSLALFGRNLTNHKGLSSTLPVAGLFTFGAAQAPRTYGVELGFRF"
     gene            15140..16597
                     /locus_tag="VN034_13620"
     CDS             15140..16597
                     /locus_tag="VN034_13620"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWV61696.1"
                     /translation="MTQAFFDHPFLSGHHQPVRFEATAPDLIVEGEIPADLVGVFYRN
                     GPEPLYPTREGEYHWFDGDGMVYAFHIADGRASMLNRWVRTEKFELEQAAGERLFGLF
                     GNPMTADPSVQGKRYNTANTNIIVHGGKLLALMEGAPAVALDPRTLETLGEEHYGGTI
                     TTTFSAHPKIDHYSGELVNIGAMVGGPMGAAQIRYDVIDASGQVRKAELIDMPHNALM
                     HTFFLTENWVVFPVIPIDVDLGRFMKGGPMTAWVNDRPTKFAIMPREGSASDVRWFDY
                     EPRHMFHELNVWEQGGKIIADVAAANGTALFPDETGVKRTHGDTQQSLRRWTIDPGVG
                     GNGTWIKEETLNDRDIQFPRPDDRFMTRASRHSFANINLNSRDGRAEGMDGVLRFDTI
                     SGTEDVYHFGNGASCGELIFAPKIGAQGEGDGYAMTLVHRAGAATSELAIFDALDIAA
                     GPVATVHVPFRVPSGFHCNFYAADSMLYRQAFGAA"
     gene            16610..18190
                     /locus_tag="VN034_13625"
     CDS             16610..18190
                     /locus_tag="VN034_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HWV61697.1"
                     /translation="MKVAPFECVADVARRNGRERPDKILFHFEGRATSYADFNTNSNR
                     AANALVALGLKPDDRVAYLGKNSDLYFELLMGVAKAGGVMTPVNWRLAEPEIAWIIDN
                     CGARILCVGPEFTELVAANRTAFPGVEHVIPVEGAANGFPDYRGWRDTFADTDPDVPR
                     GEGDAVIQMYTSGTTGKPKGAVLTNGSVLRSKLDRDPALQADWERWDADDVGLVAMPC
                     FHIGGTGYGITVMTNGATGVITREFDPGGVLDLIEAYGISKIFMVPAAMQIIVGQPRA
                     REVDFSRLRHIAYGASPIPLDLLRQCIDVFQCGFVQMYGMTETSGTIAALPPEDHDPA
                     GNERMRSVGKPLRGVEMRIIDAEGNPLPPRKIGEIAIRTQANMREYWGRPEATAETID
                     ASGWLRTGDAGYTDEDGYFYLHDRVKDMIISGGENVYPAEVENAIYGHPAIADVAVVG
                     VPDDKWGEAVKACVVLREGASVEAGEIIGWARERIAGYKCPKSVDFIPTLPRNPSGKI
                     LRRELRAPYWEGRERQIS"
     gene            complement(18183..18995)
                     /locus_tag="VN034_13630"
     CDS             complement(18183..18995)
                     /locus_tag="VN034_13630"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61698.1"
                     /translation="MGRLAGKRAVIIGAASTGNMAQVTARHFAREGATVMVAGRKAEP
                     LDALAAEIGGHAALCDITDKASVFALADAAKAKMGGVDIAMNASGWGLLKPLLDVTEE
                     ELAAIVALQFTGVHHFLQAFVGAMLANEPTGGSIIQISSATTRSPISNHAAYIGTKTG
                     SEALIRCVANDFGAAGIRANIVSPAFTESPMTEGAFATPGLVDAFLPRYPLGRLNTAQ
                     DVANACLWLASDEAFVTGENLQPNGGLALRGNPQEADIAAAVGAAMAKLQAS"
     gene            complement(19006..20004)
                     /locus_tag="VN034_13635"
     CDS             complement(19006..20004)
                     /locus_tag="VN034_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWV61699.1"
                     /translation="MPTSRQWLLNGHPRGRGIEDGDFRLVETGIGAPGAGELLLKTLY
                     LGFDPAQKGWMENIADYVAPMAIGDVMRGSGISEVVESNHPGFATGDLVMGSTGWTEW
                     HVSTGEGLVKIETELPPTAMLSVLGTTGVTAYCGLFKVGRPVAGDTVLVSGAAGATGS
                     VVGQLAKIAGCRAVGIAGGEDKCRWLVEEAGYDAAIDYKAGNVRAQIKEHCPGGVNVI
                     FDNVGGAILNDMLGEIATGARVAVCGGISRYEMGTLPAGPQNYFNLIFRRGSMAGFIV
                     LDWASEFPEIRKRLEGFVLDGRLNYREDVQHGFENAPDTLKRLFAGQNRGKQLLKL"
     gene            20122..22245
                     /locus_tag="VN034_13640"
     CDS             20122..22245
                     /locus_tag="VN034_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV61700.1"
                     /translation="MPEVRRSFCRFCHANCAMLVTIDEGRVTKVQGDPDDPVFGGYTC
                     IKGRQLAEAHHGPQRLTVSQKRVDGAFQDIAMDDALDEIGAKLAAIIAEHGPHAVAIY
                     GGTYAFQNSAGVGSAMSFAQGLGTRNIYTSVTLDQPAKVYTTMRYGSWMGGMHTFAEA
                     DVAFFIGNNPIVSHYGPPGGLPPFSPSRRLRDALEGGIKLIVADPRQSDVAALAHIHL
                     PVRPGEDPALLAGIINVILSEGLFDKAFVQRYVDGLDELQRAVAGFTPDVAAARAGVE
                     ESQLVAAARMFANGTKGVVSTGTGPEMAGNGTLTQYLVSSLNIICGRFCREGEKSSIP
                     RVFTPVTPRRAQVAPPMALYGEGFPASRIRGLTHLGMEMPCNVLADEMLTPGEGQVRA
                     LIVIGGNPLVAFPDQDKMTRAIDGLDLLVCVDVNAESATAKRADYILAPKMCLEREDI
                     SNLSEWWYEIPYARYTEAMIEAPEGLLEEWEMLWELAHRLGTGMPLAGGACPMDERPT
                     KEVFLDLMVAGCSVAPSVVRADTVDGKAVIYADLHPVVEAGDPDAPGRFDLTAGAMPG
                     QLIAYADAVQPTPEFPWRMVSRRSRHRFNSTGQNLPALRAKRTTNPAFLHPDDLALVP
                     CADGDTVRIRSAVGEVVAVARAADNMRPGVVSMAHAFGGPDIGPDGVHEHGASTNRLV
                     SESVAYDPITGQSRQSAIPVSIIPA"
     gene            22255..23271
                     /locus_tag="VN034_13645"
     CDS             22255..23271
                     /locus_tag="VN034_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWV61701.1"
                     /translation="MPDNRRFLLVRRPNGEPVQADFSLITEPTPELADGQFLIRNHYA
                     SLDPAIRGWMDDAPSYMPPIPLGDPVRASTIGIVEASKADGFAPGQWVLGLNGIEDYS
                     VGTVGGFTQPIDARLVPSVTNYLSVLGAVGMTAYFGYIDVCEPKPDDVVLVTGAAGAV
                     GSLVGQIAKIKGASKVIGIAGGPEKCAKLIDRYGFDAAIDYRGKDLAALTDAIRAEAP
                     DGVDIIFENVGGDILDAGLMNLRHGARIGLCGLISEYNSVEKVGARNIWQLIVHRASI
                     RGLLVADYIPRFAEGGAEMANWLQQGKLLADEHVDEGIENSFDAFMRLFAGSNQGKMI
                     LKIA"
     gene            23268..24044
                     /locus_tag="VN034_13650"
     CDS             23268..24044
                     /locus_tag="VN034_13650"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWV61702.1"
                     /translation="MSPPRRLLVLSGGHPYDAGPFADLLASFTGWTVTHLIHPEAETG
                     VAEGAANNTDAILFYDMPGYRFEGGKAATRPPSFTFRRAITDYFARGGGAVAMHHAIA
                     GWADWPEWADMLGGRFLYAPGEANGENHLDSGYRHDVRYDAVVLDPGHPVTAGLPPRF
                     PLCDELYLAPVWGAVAPLLRADHAFVRNNFYSAAQAVAGRMFSNAGWDHRAESDLIAW
                     EKPVGAGRLVYLQPGDGSAAYADPNLRRFLANALTHVAAA"
     gene            24120..25364
                     /locus_tag="VN034_13655"
     CDS             24120..25364
                     /locus_tag="VN034_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV61703.1"
                     /translation="MNDIAASSFFDPQVIADPFDYYRAWMPKSPVVQLSEGMFLVLSY
                     DLCSQATGEVETFSNNFQGTLSGAMAEDGDVAAILAEGWPQVDTLLTADPPTHTRFRK
                     LVNLAFSMKRVAAIEEDMRDVVIDLIEKMAAKGEADFVRDFGIPLPVAMIASQIGMEG
                     DLDRVKRWSDAFVDRLGRMIPKERELECAREVVEFQHYVKAKIDQRRANRTDDLLSDL
                     VYAEVDGERPLEDAEILSIMQQLMVAGNETTTSALAGGLLQLIGNPDQMAKAVAAADE
                     GNERAIINLVEEVLRTESPTAGMWRMVLKDAELGGVKIPKGAMVQLRYAAANRDPAKY
                     PDPDRFDIERANARSHLAFGKGIHMCVGNMLSRKEMAVAYSELLKRLTDFRVADGHTP
                     SWPPNMLLRGLTALPITFERRA"
     gene            25361..26422
                     /locus_tag="VN034_13660"
     CDS             25361..26422
                     /locus_tag="VN034_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61704.1"
                     /translation="MNFDLDEDQQLTRDMIGRFLGPVDIAARHAMRGAEGGYSRARWQ
                     EIADLGLLALAAPEAMGGIGGTMVDLALVAEALGKGIAIDPWLENGVLPIRLAAAAGD
                     DTLVGELVTGEKFAAVAFAEPGRRYETEALSTKAEGDKITGAKAFVLGAPLADALLVT
                     VAGNRLAKAKRNAAGVVEQAYPVIDGSNAATIDLHRTPADLFDLPAAKFPRVIGDVRL
                     LAAAEMLGLAQTMFDDTLAYVGERQQFGAAIGSFQAIQHRLVECYAALEQARSMVLRT
                     AMLDPTTDDANWPRLSAGAKAFVGEAATRIGLEAVQMHGGMGQTDELAIGHALKRVML
                     LDKFFGDQDHCLRSFARAA"
     gene            26419..26811
                     /locus_tag="VN034_13665"
     CDS             26419..26811
                     /locus_tag="VN034_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWV61705.1"
                     /translation="MSALAPIAPGLWTDEAEPRLIGARREDGEIVFPVPSGDAAALVE
                     PVALSRKGTLWSWTTQGFEPKEPYSGPQPFQPFLIGYVELPGEVIVETRIVDASAADL
                     IIGMPMEFAVVPFDDARSTYAFRPERQP"
     gene            26808..27956
                     /locus_tag="VN034_13670"
     CDS             26808..27956
                     /locus_tag="VN034_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWV61706.1"
                     /translation="MNDDVYIIGAGIHPFGRTDGRSGRDQGVYAVREALGDAGIEWGD
                     VEFAYGGSAAAGSADIMVNELGLTSVPFINVANGCATGGSALTAARNAIAAGACDIAL
                     AVGFDKHPRGAFNAKPSDYGLPEWYGETGMMLTTQFFALKIQRYMALHGISRRTLGLV
                     AEKAFRNGTLCDHAWRRSPVDLDTILNAPMVNDPLTKYMFCSPAEGAVALILASGRKV
                     RELGADAVRIASVAFRTRPEGSFEVFAPSISVGGGGKPTQIASKAAFEMAGIGPEDVS
                     VAQLQDTESGAEIMHMAENGFCADGEQEQWLAEGWSEIRGRMPVNTDGGCLACGEPIG
                     ASGLRQVYENTVQLRGRGGARQVPNDPKVGYSHVYGAPGLSAVAILER"
     gene            27965..29173
                     /locus_tag="VN034_13675"
     CDS             27965..29173
                     /locus_tag="VN034_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61707.1"
                     /translation="MDLDLTKDELIFREEVRDFLRTSLPDYVRDGARRTPGVFVEPDI
                     GLVWHRILNDKGWVAYHWPEDCGGTGWTPMQRYIFEKECAIAGAPALSVLGLRLVGPV
                     ICAFGTQEQKDRFLPAIRAGTDYWCQGYSEPQSGSDLASLRTRARRDGDEYVVDGSKI
                     WTTHAHHANWIFCLVRTDGEVKKQAGISFILVPMDQPGVTVRPIITMAGDHEVNQVFL
                     DGARTSVDNRIGEEGQGWTIAKFLLENERGGSFHAPRLIRAIDHLEQLAGVAPSGHNG
                     ALGGDIQIATRIAKLRLEAEALETTELRILADIAKGRPAGPQTSLVKLLSSEIHQRID
                     GLAMDLHGYDGLQLPTVRPLYGNEAPPAIGDEDAQVAAARYLNSRAWTVFGGSSEVQK
                     NIIAKTVLRL"
     gene            29266..30036
                     /locus_tag="VN034_13680"
     CDS             29266..30036
                     /locus_tag="VN034_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV61708.1"
                     /translation="MDARTLVDAVTVRSVEDIHDAAVVLRDYAAAYGLRAALCDDIAS
                     KETMVDADGAVLAADLFGWLGDGERWWEDHRLALHSPLPRSCRYESEPFWCNAEGFRT
                     ANPNPYLDDIDLSRYFSAHERYKAAIVVPVHLPFGQISANSFQPSDPAITDLSDMFAA
                     IGDTLALATRRFIAGYASAMRTKRRIPSDCELSKREVECLRWAAIGKTDREIGMIISL
                     SHATVRYHVHRAGEKLNSVNRAQTIFKAGQLGYLGANS"
     gene            complement(30033..30569)
                     /locus_tag="VN034_13685"
     CDS             complement(30033..30569)
                     /locus_tag="VN034_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61709.1"
                     /translation="MPGPKSLASLGDIMQLAFVPSDFDAAIRHWTGVMGAGPFFLLPN
                     VSLPGGRYRGAPSDPVFTMALGYWGEMQIELIRPENDAKSLYRGEYAVGEGLHHMCVL
                     VESIAEARVRCDEVGAEIIFEAPVGDTGGVIYADPGTGPGTLVELLEPQAGTRELFAV
                     MRDAAKDWDGSEPLRSLG"
     gene            30668..31897
                     /locus_tag="VN034_13690"
     CDS             30668..31897
                     /locus_tag="VN034_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV61710.1"
                     /translation="MAESDPKIAPQDWFAVRKRNARAGLRKLVAIVAENRTDRAEAPL
                     PLHKSIYTGEVRHAAEMEHIFRNEPIVAGLSGDIPKTGDTLVFDSVGPSILVTRGKDG
                     VARAFLNMCMHRGAKLVEEKEPWSGPRPRLVCPFHAWTFDPAGTLVGQPGKEGFANCE
                     IGARNLIELPCAEHLGLIFVRANPDGEPIDAAAHLGDFGPVLAQLELHRAEPVKKGIL
                     TADSNWKFALDTYGEGYHFKMLHASTIGGTHYSDRNVYEPIGRHHRVSFPDLSIGNLV
                     GKDESEWPETDYGGVHFLFPNTVIFFGAVTPGVYFTQVFRLFPDGAGKMFCQFAVYAP
                     FGVESDEHRAMCEMAYDATATVVQTEDYRVASSGYANLVTAPDDFHVVLGANEPALHG
                     VHRSIAAACKMPLDQIA"
     gene            31911..33338
                     /locus_tag="VN034_13695"
     CDS             31911..33338
                     /locus_tag="VN034_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWV61711.1"
                     /translation="MNDLSPPQDQNRDDRCPGTSYTDMLNADTRRVPDYLFAESNQEL
                     GDEPIAIERYVSEDWAKRERETMWPNIWQFAAREEDMPNPGDTVVYDINEKSILLVRQ
                     KDGSVKAFYNVCLHRGRKLRTESGPAVNLRCPFHGFSWHHDGRFKEAPCAWDFKHLDD
                     KDMSLPELKVDRWQGFILVTENHDLPPFREWVGPGIEHYDNWRLDECHTGAWVGRVIP
                     ANWKAVAEAFMEAWHSIVTHPQILGFTADANTRYDLYGDFLNRAITPAGAMSPHIKGK
                     DQFYVLKSLEDFIGAGDSRARRSTASADDLKGFDADDPMLARKVLAQANRDAFSAMNG
                     HDYSDATDSEMLDNFTYNVFPNWAPWGGLTPNIVYRWRPWGDVDHCLMEVRILMRTPK
                     GEKPPRAAPMHLIPDDQPFASASHLIGAALAGVFDQDMENLPYVQEGMKSSPNGVIEL
                     GHYQESRIRHFHRTLGKYVGGELPG"
     gene            33338..34135
                     /locus_tag="VN034_13700"
     CDS             33338..34135
                     /locus_tag="VN034_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWV61712.1"
                     /translation="MARTVPLLSLAAGVLPEFSPEQTVAAAIAGGWPAAGIWYDPKSW
                     TPATTIEVRDRAADAGLTILDIEVIWLKPGPDDPAHFAAIDAGAAIGARNVLIVSSED
                     DTGRAAGKLNRLGNHAAAVGLRACLEFAAFTTVGDIASAVALLDAADCDNLGLLIDPL
                     HLARTGGSPADLNSVDPRRFPYAQFCDAPAHGPPPSDVAEIIREALDLRLMPGDGDLS
                     LDDLLAQLPPTTPLSVELRSAALRDDHPDAADRARSLLAATQRWFAA"
BASE COUNT         6126 a        11652 c        10633 g         5733 t
ORIGIN      
        1 atggtcgagc gcgccgacga gcgccatcga acgcacgatg cgcagcgcca tgtcgcgcac
       61 acgcgccggt gcaagctcgc ccgccttcac ggcggcgggg aggccggcga aataatcctt
      121 gtcctgcggc gactgctggt cgagcccggc gtcgatcgac ggcttcaggc tatgcaccgc
      181 gccccagtcg gacatgaccc agcccgaata ttgccagtcg cccttcagca cgtcgcgcat
      241 cagaaattca ttctcgcacg cataggtgcc gcccaccttg ttgtaggcgc acatgatcga
      301 ggcgggcttg ccgcgcttga taccgatctc gaaggcgagc aggtcggatt cgcgcagcgc
      361 cgcctcgccg atccgcgcgt catagacgaa gcggccgctt tcctgcgggt tcagcgtaaa
      421 atgcttgatc gtcgaggcga cgtgacgatc ctggataccc gcgatctgct cgccgacgag
      481 gatgcccgcg agcagcggat cttcgccaag atattcgaaa ttgcggccgt tccgcagttc
      541 gcgcgcgaga ttgacgccgc cggcgagcat cacgttgatc ccctttgcaa aggcttcggc
      601 gccgatcagc cgcccggcct cgcgcgccat cgcgggatcg aaggtcgccg caagcgacag
      661 cgtcgcgggc agtgcggtcg attcctgacc ggggcgcaga tagccgatgt cggcgacccc
      721 gaggctcgca tcgccaagat gcagagcggg aaaattcacg cggccgatag cgggaacata
      781 acccgcggtg ccgatcgcgc catcgggttt cggctgccgc atcgcttcgg ggatgaaggg
      841 cgggagcgga atgccgaggt cgagcaggct gccgcccgac atcgatttgg tcagcgaaat
      901 ctgctcgtcg agcgtcatcg catcgacgat cgccgcggcg cgcttttccg ccgtttttgc
      961 ttgttcggtc attccgtccg cctgtttggc cgtcgccggc atggcaataa tcgtggcaac
     1021 cgccagaatg gcggtggaca tggcgcgtcc catttccctc tcctgctatt tgattttttc
     1081 ggggcctacc gacagcatcc cggtttcaag gccggccgcc atcaggcgtt tgagcacctt
     1141 caccgaatcg cgcacgctgc ccggcgcggg cgccagcacc tgcgcgattg acaggccgcg
     1201 gatcgcccag acgaccagcc gcatgacggc catcgccccg cgtgcatctt ccgccgcggc
     1261 gagatgtgtg aattcgagcg cttctttctc gatccgcgcc tgcacgggcg cgagcatctc
     1321 gcaaagctgc gggtcgctgc gcgagccctg caaaatctcg agcaccgcga cgcccgatgg
     1381 gcggctcagc acctcccatg ccatttccgg ataggcgagg aagcgttccc taggatcagt
     1441 gacgtcgcgc agattttccg aataatgctg cagctcggcc tcgaacaccg cctcgacaac
     1501 gaacgtcatc agatcggctt tggtacgaaa ctggtggagc atcgcgccgc gactcacccc
     1561 cgccttttcc gcgacgagga tcgtcgaggt cgcggcatag ccatgttcgt gcaacgcctc
     1621 gatcgcggcc gccaaaagca cctcgcgcgt ccccgcgctc cgctccgcct gcgtccgcct
     1681 ctgcggtttc tcggccatat cttcgcctct ccatttacaa gcagtattga ccgtaacata
     1741 tgggcgccat aggatcatgg caagcataaa ggagcacgcc atgatcgaac gccgccagtt
     1801 tcttgccgcc gccgcggcca ctgcgatgct tcccaaaagg gccattgcgg ccccggccaa
     1861 gccgattatc ggcttccagc tctacatggc gcgcgaactg ctcgcgaagg acttcgaagc
     1921 gacgctgacc gccattgccg cgacgggggt gcggcacgta gaattcgcgg gcttctatga
     1981 ccgggacgcc gccgccatcc gcaagactct tgccgacaac ggcctgatcg cgatcggcgc
     2041 ccatgcggtg cgcgccgaca tggacgatgc cgagatcgac cgcctgatcg agcaatgcgc
     2101 cgcgatcggc atggagtatg tggtcgcacc cgtgccgctc gttcccgcct atcgcgcgct
     2161 ggtcggcaag tccgacaacc cctttcgcga tgcgatagcc gcgctcaccc gcgatgattt
     2221 cctgcgcacc gccgaccgct tcaacgccat cggcggcaag gtccagaatg cggggatgcg
     2281 tttcgcctac cacacccatg gcctcgattt cctgcgcttc gacggacgct atgccttcga
     2341 cgacatgatc gaccgctgcg accccgcgct cgtcgccttc gaactcgata tcggcaacac
     2401 catcgcggcc ggggtcgatc cccttcccta cctcgaacgc ctcggccgcc gcgcggcgct
     2461 cgcgcatctc aaggattggc agggtccgat cgagcccgca gtcgaccggg tcccgccgac
     2521 cgccccgatc ggcgaaggca tcgtcgatct caaagcaacg atgcgggcga tgaccgccgc
     2581 gggcgtgcgc tatgccttca tcgaggagga agagacgccg gcgggccgcg tgatcgccgc
     2641 catcggttcg gcctatcgcc atctcagccg cctatgacgc cttgcccgcc gccgacgcca
     2701 gcgcgcgttc ggcgatcggg cgcatcacgg catagcctgc cgcggtcggg tggacgccgt
     2761 cttcggcatg aacctttgcc aatccgtgcc cgccgtcgtc gagggcggca taataatcgg
     2821 cgaagaccag cttccgctcc gccgccagcg cccgaaggcg cgcgttcagc gcggcgatcg
     2881 gctcgatcgg agcgatttcg ggccgccagt tgaattgacg ggcgggaagc accgagccga
     2941 tcaccaccgc gatcccatgc gcttcggcga ggtcgaccat cgtgcggata ttgccctcga
     3001 tcgtatcgag cgaagtggcg ccgaggttcc cggcaatgtc gttggtgccc gccatgatat
     3061 ggacgacccg cggcttgaga tcgatcacgt cggtgcgaaa tcgcgcgagc atctgcgtgg
     3121 tcgtctggcc gctgatcccg cgatcgatgc ggtcgccggt gaagaagccc ggctgccggg
     3181 ccagccatgc ttcggtgatc gaatcgccaa tgaagacgat ccggtccgat gtggccggcg
     3241 ccaacgcccg attcgccgcg gcgtagcggc acaggcccgc aaagtcggca aggagcagat
     3301 cctgctgcca tctcgcatag atagccatgc cgtcctcgga acgcggcggc agtggatcgc
     3361 cggcagcaag cgcagccttg gcgcgcacca tatagtcggc gacgacggcg ggcgtcggcg
     3421 gcaaagcggc acaggggtcg gcaacaacgc cgacccgcgg cgcaggctca cccgctgcca
     3481 ccggcagcga aacaagcgcc aacggcaaga aaatcaacga tttcatcagc cacctcctaa
     3541 aatatctttc ccgataccct atcacccgaa tgataactta caagcggtct tgactgcttt
     3601 tatgttgcaa gcctatcaag cggcaccggc gccgcgagcg ccttgctggg gaggttatca
     3661 tggttcgatt tggcgcgcgg cgcatattgc tcggttcggt tacggcgatg gcgctgcccg
     3721 cgatgccggt gttcgcgcag gacgtcgaac ccggcgcggc gaccaccggc gacgagatca
     3781 tcgtcaccgc gacgcgccgc gaggagcgtt tgcaggatgt tccgctttcg gtgaccgcgg
     3841 tctcgggcga aagcctggcg cagtcggggc tgaaggaagt cagcgacatc cagtatctcg
     3901 ccccgaacat caccttctcg gcgaccaacc ccgtatcgaa cggcggcggc taccagatcc
     3961 gcggcgtcgg cacccagacc tatgacagcg gcgtcgagca gaccgtcggc ctcgtcatcg
     4021 acggggtcgt catcggcctg tcgcgcgatc ccggttcgac cggctttgcc gatatcgaac
     4081 gcatcgaagt gctgcgcggc ccgcagggaa cgctgttcgg caagaattcg agcgcgggtg
     4141 tcatccaggt cgtcaccaaa cggccgcgca tggacgacgc ctccttctcg ctcgacctca
     4201 aatatggcga gcgcaatgat cgcgtcgtac aggccagcgc caatgttccg ctcggcgaaa
     4261 ccctcgccct gcgcatttcg ggctatagca atgggcagga cggcgcgatc ccctatgtgc
     4321 tgaccccgtc gcgcgccgtc ggcgaccgcc ggaacaacgg cgttcgcgcg aagctgctct
     4381 gggagccgtc ggacaatctc tcgttcctgc tgtcgggcga atatcagacc gcctttgccc
     4441 gcgacggcca gatcatccag tcgctcggca ccagcgccct gttcaattcg cagttcgccc
     4501 gctttgccga gaagcccggt ccgaacgtct acaaaagcta tatggatggc gactggacag
     4561 ccgataccaa gctctacgcc ggctcgatcg aaatcaacgc aggtctcggc gaccacatgc
     4621 tgacctcgat caccgcttac cgtaagctgg agacactcca gctatccgac atcgatgcct
     4681 cgcccgcgaa catcttcaac aacagcgacg gcggcgtcga cagcaatcag ttcacgcagg
     4741 agcttcgcct gacgtcgccc ggcggaagcc gcctcgaata tgtgctgggg ctctattatt
     4801 accggaccga gaatggtgga tatgccgtcc aatacggaaa ttattacggg cttttcggcg
     4861 ctccggtggt tgtcggcggc ggacagcgcg atgcgacgaa caaggtgcgc agcctcgccg
     4921 cctttggcaa tgtgaccttt gcgctgaccg acgcgttcaa gctgatcggt ggcctccgct
     4981 acaccaacga ccgcaaccac ggcacgctcg tcgtctcgcc gctgcccttc ccggcggtcc
     5041 cgttggggac gctgccgaat tacgacggca cggtgaaggc cgacaacgtg tcgggcaagg
     5101 tcggcgtgca gttcgagccg agccgcgact tcatgctcta cgcgacctat tcgaccggct
     5161 tcaagggacc ggcgatcgac ggcaccgccg gcatcattcg cgaggttcgc cccgaaacgg
     5221 tgaagagctg ggaagtcggt ctcaagagca gtttcctcga cggcaagggg acattcaaca
     5281 tcgccgccta ctggtcgaac taccgcgatt tccaggcgca gaccttcgac atcacgacga
     5341 cgccgccgac attctatctg tcgaacgccg ggctgcttcg cgcacgcggt atcgaggtcg
     5401 aaaccggctt tcgcgtgacc cccgcgctgc gcctctccgc gagcggatcg tataatgacg
     5461 cgaccttccg cgaatatgac gggcaatgct atccgggcca gccgatctcg ccggtcgtcg
     5521 gccagggctg ctatgtcgtt cccggcacga ccacgacggt cgccaactac cgcggcttcc
     5581 gcctgcccaa tgcgccgaaa tggtcgtaca tcctgcgcgc cgactacaag caggcgatcg
     5641 gcggcgacat ggcgatcgac gctgcggcca attgggcgtg gcgcacgaaa acgcaggccg
     5701 tgatcggcga tccgaacgcc gagattccct cctatggcct cttgaacggc accatcggat
     5761 tcggcgcgga agacggcagt tggcggttcg gcatctacgc gcgcaacctg ctcgataagc
     5821 gcttctacgc cccctatgcc gccggcgtga tcaatcccgg cggctactcc aaaatcgtga
     5881 tgcccgaagc cttccggacg atcggcggca cgctgagttt ccgaatttaa ttgttgctat
     5941 ccctgccctc cttccaccgg tgaagggggg caggccctcc cgagtaacga ctaagtctct
     6001 tgcttctccg ttgcatttct cgtgcttcga ggcggcgttc gctttagacg ccgaatactg
     6061 gattgatgcc ggttctcgag tgaggctcgt catgctccgc tactgctgtc gaccgaagcc
     6121 ggacagcacg cactggaccg ctataaaaaa tttctcgcgg cccacccttc gacaacaaat
     6181 ccccacgtca cgcgaatgaa gtagccttag gcaacctgtg tcgcgcccga ggtcagacag
     6241 gattggccgg tagcggaccg tatgcttccg gatcgcaaca gaaaaagcgg actggaaagg
     6301 accaggcgtt cccgaggcat cggaggccgg cgcccgtgtg gtcatcggct cgataacttc
     6361 cggacatgga agagctcatg atatttgcgc ggttgcgatc gcagatactg tggaggcact
     6421 tccaccttag cccccaaatg cgcggcagcg tgccatggcc aacgcgggtc gaacaatatg
     6481 ccacgcgcca atgccacgag gtcggcatcg cctcccgcga cgaccgcctc ggcctgatca
     6541 taatcggtga tcaggccaac ggcgaccacc ggcatcgaca cggcctgctt taccgcgcgg
     6601 gccagcggaa cctgatagcc ggggctcacc ggaatattct gccgcggatc aaggcccccg
     6661 ctcgatacat ggatagcccc gcaaccgcgc gtttccaagg cctgggcgaa ctcgatcgtc
     6721 tgttcgacgg tccacccgcc ttcgacccaa tcggtacccg acacgcgcac tgtcaccgcg
     6781 cgctctgccg ggaatatgtt ccggaccgca tcgaagactt cgagcggaaa gcgcattcgg
     6841 ttttcgagat tgccgccgaa ttcgtcggtt cgtcgattcg agagcggcga aaggaactgg
     6901 tgcagcaaat agccatgcgc cgcatgaagc tggatcgcat cgatgccaag ccgcgccgcg
     6961 cgcaccgccg ccgccgcgaa agcatcgcga acacggacca gaccgtcgcg gtcgagctcg
     7021 atcggcgcga tgtccccttc cgcaaagcca aacggcgacg ccccctcggt ccgccaacca
     7081 ccggcatcgt caggccctaa ttgggccccg cccttccacg gcacctcggt cgatgccttg
     7141 cggccggcgt ggccgagctg gattgcgatc ggcatgtcgg accagcgacg gacgctatcc
     7201 agcacgcgcg ccatcgcggc ttcggtcgcg tcatcccaca ggccgacatc ggcgtagctg
     7261 atccggcctt cgggcagcac cgccgtcgcc tcgatcgtca gcagcgccgc gcccgacagc
     7321 gcgagattgc cgagatgaat cagatgccag tcggtcatgc aaccattgac agccgaatat
     7381 tggcacatcg gtgcaatcac gatgcggttg cgcaaggcaa ggctgccgac cgcaaatgga
     7441 gtaaacaggg cggattgggg caagatggac tccgaattgc ggggcgggct cgccctagac
     7501 acccatcata ccggcggtgg tcgcgccgtc gatgacatag tcggcgcccg agataaaacc
     7561 cgcttcgtcg gatgccagaa acgcgaccag cgcactgacc tcacccagct gcgccatacg
     7621 cttcatcggc gccatcccct cgaacgcggc gcgcgccgcg gcggggtcgg gcgcacggtc
     7681 gatcaccccg cggatcatgt cggtttcaac gacgccgggc aggatcgcgt tgacgcggac
     7741 ctgatagccc ttgcccgcgc aatggatcgc ggccgatttg acgagcgcga ccaccgcggc
     7801 cttggtcacc gaataggcgg cataattgcc catcgcgcgg tgtgcattca tcgacgagtt
     7861 gacgatgatc gaacccttgg gaccttgcgg gttcttcgcc atcgcgcgga tcgcgtgctg
     7921 gacggtcagc ataaccccgg tctggttcac atcgacgata tggttccacg cgtcgatgcc
     7981 gatgtcctcg acgctcgcgt cgcccgcttc ggtcccggca ttggcgaagg cgatgtcgag
     8041 ccggccatat tcggcctcga tctcggccat cagcgcatcc cacgccgacg cgtcctcgac
     8101 acggtgcgcg cgaaagatcg cgccggtttc gccctcgacc gccgcagcgg cttcggcatt
     8161 cgagcccgtg aagaccacct tcgcaccgtc gcgaaccagc cttgcaacgg tcgcggcacc
     8221 gatgccgctt gtcccgccgg tgaccaaggc gaccttaccg ccgagcttct gctgcatcgc
     8281 gcctctcctc tcgctgtttc tcttgccagt ttcgcagcat cggtgaacct atcaatacag
     8341 caaggtgacg cgggtgcgca gcgaacgcat gttgcgccga caacaggagg agaggccccg
     8401 cgatgagcga cagcgaacat cccccccgca tcgattgcgt gctgatctgc ggcggcgtct
     8461 ggcacgacat ggattttgcg cggctcgagc tgttgaagtt gctcgccgaa gatcagcgtg
     8521 tgcgcacgcg cgtgttcgag aattacgaga atatcgaggc gatcgaggcg gccgacatcc
     8581 tgatcaccta cacctgcgac gtcacgccct cccttcccgc gcaggaggcg ctaaagtgct
     8641 ggctcgcgaa gggcgggcgc tggtacgcgc tgcacggcac caactcgatc ctccgcctgc
     8701 tcgatacggg cgtatgggat gcgccgcgct gggcgccgct gatgatggac ttgctggggt
     8761 cgcaattcat tagccacccg gcgatcgaac catacactgt ccgcgtcgcc gaccccgatc
     8821 atgcgctcac caagggtatc gagccgttcg agacgaccga cgagctttac catctcgaaa
     8881 cgcatgggca cctgcacgtg cttctcgaca ccgagtgcac cgggctcggc acgggcttcg
     8941 tcgaggccga ggatgcgccc ggcacctatc cggtctttta catcaagcag catggcgcgg
     9001 gcgcggtgct ctacctcacg ctcgggcatt gtcgtggtca ttacgacctg cagccgatga
     9061 tggactggtg gccgacggtc gaccgctgcg cgtgggacct gccggtcttc tatgacctgc
     9121 tgcgccgcgg gatcggctgg ctcaaggagc gcgagagcgc ctaggctttc tccataaaga
     9181 tccgttcgtg ctgagcttgt cgaagcactg tccttattct cgcggcgccc gaagaaagag
     9241 cggcccttcg acaagctcag ggcgaacggg aaattaggtc tgcctttcag gccaccgcgc
     9301 gcaatttccg ccggtcctcg cggatcagat cggcagcgcg gagcgcgagc gccatcgccg
     9361 gcgcattggt gttgcccgtc accggcgagg gcatcaccga acagtcgacg acgcgcagtc
     9421 catcgacgcc tttgacgcgc agccgcgcgt cggtgaccgc gcgattgtcg ctgcccatcc
     9481 ggcacgtccc gaccccatgc agcccgcagg tcgcgagacg gcggaaagcg gcaaggattt
     9541 cggcgtcgct ctgcaccgcc gcgcccggga gcagttcgcg ctcgaccaac ccgacaagcg
     9601 ccggctgcgc catatagcgg cgcatcgcgt ggacggcggc gatcgcggtg cgttcatcgt
     9661 cgcgcgtcga cagccagttg ggtttgatgt cgggcgcggc atcgctgccc ggtcccgcga
     9721 tcaggatgct cccctcgctc gtcaggcgca ggagctggcc atagatcgtc attccgggct
     9781 tgcggtcgat gctgttcagc ggaaccggat gcttctcgtc gccgagcgca aaagtgtagc
     9841 cgccgagata gagctggaga tcgggccgca tgtcgcggct cgtcaggttc aggaaggcgc
     9901 cgacctcgaa cggccccgtc gccatgatcc cgccgcgcgt cagccaatat tgcgccatcg
     9961 cacgggcgag gccgatgcca tagaaactgc ggttgctgcc gatgtcgcgc gacaggcggt
    10021 gcggcatcga aaagcccaga tgctcgcgca tccgcccgcc gacgtcgggg gcatcgacga
    10081 ttggctcgac cccagcggcg cgcagcacat cggcggggcc gatgcccgaa cgctggagca
    10141 gcaacggact ttccagcgtg ccgcccgaaa cgatcacctc gccgtcgcag tcgaactgct
    10201 gcggctgtcc agcgaccttc gcctcgacgc ccgtcacgcg tccgccttcg accaccagcc
    10261 gctcggccat cgcgtgcgtc acgacgtgca ggttcggccg tttcttcgcg cgcgcgagaa
    10321 aggtcgccgc cgcgctctcg cgccggccgc gccggacatt gtgcgaataa aagccgacgc
    10381 ggtccgacgt tgccgcgttg agatcatcga cgggcttcag tcccatctcg acgccggcct
    10441 gaaccatcgt ctcggcaagc ggatagctgt gaatcttggg cgcgacgccg actggcccgc
    10501 ccgtgccgcg catcgcgctc gccccaaggc catggtcttc caggcggcgg aaggcgtcgg
    10561 tcatcgcctc gccgttccag ccggtgcagc cgagcttctg ccattcgtca taatcggcgg
    10621 gctcgccgcg gctccagatc atgccgttga ccgccgacga tccgccgagc cccttgccgc
    10681 ggatccacac ctcgctcggc gccgcgccgt cgccgcgcgg ctgcgacacg ccataggccc
    10741 agatatgctc gggattggtg acgagcttcg cgacgccctt cgggatcgca acccagcggc
    10801 tgtcattgct gccgcccgct tcgagcagca acacgcggac ttgcgggtcg gcgctcaatt
    10861 gctcggcgag cacgcacccg gccgatcccg cgccgacgat gatatagtcc cagcctgccg
    10921 acatgccgcc tctcctgcaa cgggccatgc aaccttggaa ccgtcgacat gcccctcgca
    10981 cctagcgcaa taaatagtcc cctccccctt cgcgcaggat gatagtgccg cggcaaaaga
    11041 gggaggatca catggcagga gcaagcggac gggtcgcggg caagacggcc tttgtcaccg
    11101 gcggcgcgtc ggggctcggc ctcgacatcg ttcgccgctt cgtcgccgag ggcgcggccg
    11161 tcgtcatcgc cgatatcgat gttggcgcgg gcgaggcgct cgccgccgaa ctcgggcagc
    11221 gcttcgtcag gctcgatgta tcgaacgaag cggaatggct cgcggcgctc gacgcttgcg
    11281 aacgcatcga catcctcgtc aacaatgccg gcattacaac gcacgggtcg atcgaggatc
    11341 tgacgctcga agcctttcgt catgaatttt cggtcgacgt cgacggcgtg ttcctcggct
    11401 gcaagcacat aattccgaag atgaaggatc gtggcggatc ggtgatcaac atgtcgtcga
    11461 tgtgcggcgt ccgcgcgcag ggcgaccttg ccgcctacaa cgccgcgaag gccgcggtga
    11521 cgcacctcac caaatcggtc gcgctccatt atgcgcgcaa aggctacggc atccgctgca
    11581 attcggtgca tccgggcgcg atccacacgc cgatcctcga caaggtgatg gcgcaggtcg
    11641 ccgacggcca ggcgctctac gacagctggg tcgcgacgca tcccgtcggg cgcctcggca
    11701 agcccgagga gatttcggcg atcgtcctct acctcgcgtc ggacgagtcc gcctttgcga
    11761 ccggcgccga atttcgcgtc gacggcggca gttcgctgtg atggcgggtc gcctcgacgg
    11821 acgcgccgcg ctcgtcaccg gtgccgggtc gggaatcggg ctggcgacgg caaggcggct
    11881 tgccgccgac ggcgcgcgcg tgctgaccac cgatcgcgcc ggcgaggtcg atatcaccgc
    11941 cgatgtgacc gcgcccggta tcaacgcgaa actcgtcgcc gaagccgagg cgcgcttggg
    12001 cgggctcgac atcgttgtcg cctgcgccgg tatcaccggc atgcagatgc tcgacggaca
    12061 tgacgacgaa tttttcgacg cgatgatgac tgtaaacgtc acagcggtct tccgcctgat
    12121 gcgcgacgcg ctgccgctgc tcaaacgctc accgcacggc cggatcatcc tgatcggatc
    12181 ggtcatgtcg agctttggcg aagcggggat ggcggcatac agcgcgtcaa aacatgccgt
    12241 gcttggcatg acgaaatcgg tcgcggcgga ggtcggcgcg ttcgggatca cggtcaattg
    12301 catccagccc ggcgcgatcg acacgccgat gaccgcaccc gccttcgccg cgatgcccga
    12361 attcagacag cgctggatcg acaaggccgc gctcggccgc ctcggccagc ccgaagacat
    12421 tgccgacgtc gcggcctttc tggcctcgga cgaggcgcga ttcatgtccg gccatggcgt
    12481 tttcgtcgac ggtggagcga cacaacgcca gtaaaatcaa cgcgtttgcc gccgctacgg
    12541 catgcctgat atttttgatg attgttgaat cggggcattc agcggacctt cccctccgac
    12601 gtgactggcg agcctatgcg ccggcgcgag gagggaggaa cgcgatgatc acgcatgccc
    12661 gaatgctgcc tgccgtttac gccaccgtat cgctcgccgc actggcgatc gccaccccgg
    12721 ccgcggccca agagtccccg gtcgaagaaa gcagcggcgg cgccatccgc gagatcgtcg
    12781 tcaccgcgca gaagcgcgaa gaaagcatcc agtcgatccc gatcgcggtg accgcgctcg
    12841 acgaaaaggc gctggaatcg gcgacgatcg acgacatccg cgacatcgcc ggccgcgtgc
    12901 ccagcctcgt cgtcgactcg gtcggcgcgg gcccgagcgc tgccgcgatc gcgatccgcg
    12961 gtatcagctt cgaggatatc gaaaagagct tcgaccccgc ggtcggcgtc gtcgtcgacg
    13021 gcgtgttcat cggcaccaac accggccagt tgctcgacag cttcgacctc gaacgcctgg
    13081 aagtgctgcg cgggccgcag ggcaccttgt tcggccgcaa cacgatcggc ggcgtgatca
    13141 acgtcatccg cacccgcccg accgaggatt tcggcgtgcg cggccagttc gcctacagcc
    13201 gcttcgacac caagcgcgcg cgccttgtcg tcaacaccgg caagctcggc gatttcatcg
    13261 ccttgaaggc gttcggctat tacgacaaga ccgacggctt ctactacaac gtcaccaagg
    13321 atcgccgcga aggcaaatat gaaacgctga ccggcggcgt caccgcgttg atcacgccga
    13381 gcgacgcgat caccgcgcag atcacctatc agcacacgcg cgaacgcggc gaaaccgtcg
    13441 cctcggcgct gtccgaaacc ggccgcgatg tcatctgcgc cgcccccgga gcgcccggct
    13501 tttcgcccgc cgaggaatgc aaccgctggt cgctgcccgc gcacgggctt tatacgacat
    13561 tccagaatat cgagacgccg gtgcgcaaca atgtcgacag cgtcacgggc aatatcgaca
    13621 tcgacctcag cgacaatctg acgctgtcgt cggtcaccgg ctacatcacc aaccgcgaat
    13681 cggtgacgca ggatttcgac ggcagctcgg ccgatttctt cgacacgcgg cgccagcaga
    13741 aatatgagca gttcagccag gaactccgcc tgctcgccga cttcgacacg gtcaacgtcc
    13801 tcctcggcgg ctattatttc gacagcaact acacgctcga ccagtcgacc aatttcggcg
    13861 tcgtgctcgg cgggggatcg accgcggtgc ttcgtcaata tgtcgaccac agcgccaaat
    13921 cctacgccgg ctttgccgat gcgcagatca gcctgaccga cgcgttcaaa atctcggtcg
    13981 gcgcgcgcta tacgaaggac aagaaggcga tcttcaacaa ctacggccgc atcggtgcgc
    14041 tcgtcggcat caccctgccg accttcgacg gcacgtcgtg cgtcgcggtg accggcacca
    14101 ccagccccgc gccgggcgtc gtcatccccg tctatagccc cgcggacaat tgctcgggca
    14161 gcgacagctt cgacaaattc acctggcgcg ccaacgccga atatacgatc gaacccggca
    14221 agctcgtcta tgcgtccttc tcgcgcggtt tccgttcggg cgggttcaac ggccgcgcgg
    14281 cgtcgccgac ctcgctcggg ccctatcagc ccgaaacggt cgacgcctat gaggtcggat
    14341 tgaaggccga ctggctcgac cgcacgctgc gcaccaacct cgccttctat tacaccaaat
    14401 atgacaacaa gcaggaagag gtcgtgcagc cctcgcctcc gggttcgtcg agcccgcagg
    14461 aaacggtggt gcgcaacgcc tcctcggccg atatcaaggg tttcgaggcc gaaatcatcg
    14521 cgcagatgac cgacgcattc agcttcaacg cctcgttcag ttacaccgac gccaagtata
    14581 agaatttctt caacgacatc gtcggcctga ctccgggcag cccggccgat ggcattgcgg
    14641 acgatgtctc gacgctgacg ctgcgccgcg cgcccaaatt ccagtggtcg gcggggctca
    14701 actattcgaa ggagatcggc tcgggccgct tcgatgcatc gacgctgcta cgctaccaga
    14761 gcaaatatgt gacctgcatc gcaccgaacc ggccggtcgt ccccagcgcg gtcgtcaacg
    14821 acaatcgctg cttcaccgaa gatcgcgaga atctgtcggc gcagatcggt tacaccttcc
    14881 tgctcggcga cgaccgcgaa gtcagcctgg cgctgttcgg ccgcaacctc accaaccaca
    14941 aggggctgtc gtcgaccctg ccggtcgcgg gcctcttcac ctttggcgcg gcgcaggccc
    15001 cgcgcaccta cggggtcgag ctcggcttcc gtttctgaaa gcgtgaaggc tccgccaagg
    15061 gcacggcggg accgcaaaag gggggaacaa ggggcggcgc acggcaccgc cccttgttcg
    15121 acaatcgaag ggatcaggaa tgacgcaggc ctttttcgac cacccctttt tgtcgggaca
    15181 tcaccagccg gtgcgtttcg aggcgaccgc gcccgacctg atcgtcgagg gcgaaatccc
    15241 ggccgacctg gtcggcgtct tctatcgcaa cggtcccgag ccgctctatc cgacgcgcga
    15301 aggcgagtat cactggttcg acggcgacgg catggtctat gcctttcaca tcgcggacgg
    15361 tcgcgcatcg atgctcaacc gctgggtgcg gaccgagaag ttcgagctgg agcaggcggc
    15421 gggcgagcgg ctgttcgggc tgttcggcaa cccgatgacc gccgacccgt cggtgcaagg
    15481 aaaacgctat aacaccgcga acaccaacat catcgtccat ggcggcaagc tgctcgcgct
    15541 gatggagggg gctcccgcgg tcgcgctcga tccgcgcacg ctcgaaacgc tcggcgagga
    15601 acattatggc gggacgatca ccacgacctt ctccgcgcac ccgaagatcg accattatag
    15661 cggcgagctg gtcaatatcg gcgcgatggt aggcggcccg atgggcgcgg cgcagatccg
    15721 ttacgatgtg atcgatgcga gcggccaagt gcgcaaggcc gagctgatcg acatgccgca
    15781 caacgcgctg atgcacacct tcttcctgac cgaaaattgg gtcgtctttc cggtgatccc
    15841 gatcgacgtc gacctcggcc gcttcatgaa gggcggtccg atgaccgcgt gggtgaacga
    15901 ccgtccgacc aaattcgcca tcatgccgcg cgagggatcg gcgagcgacg tccgctggtt
    15961 cgattacgag ccgcgccata tgttccacga actcaacgtc tgggaacagg gcggcaagat
    16021 tatcgccgat gtcgccgccg ccaacggcac cgcgctgttc cccgacgaga cgggggtgaa
    16081 gcggacgcac ggcgacacgc agcagagtct gcgccgctgg acgatcgacc ccggcgtggg
    16141 cggcaacgga acatggatca aggaagagac gctcaacgac cgcgacatcc agttcccgcg
    16201 ccccgacgac cgcttcatga cgcgcgcctc gcggcacagt ttcgcgaaca tcaacctcaa
    16261 cagccgcgac ggccgcgccg aggggatgga cggcgtgctc cgcttcgaca ccatcagcgg
    16321 caccgaagat gtctaccact tcggcaacgg cgcctcgtgc ggagagctga tctttgcgcc
    16381 gaagatcggc gcgcagggcg aaggcgacgg ctatgcgatg acgctcgtcc accgtgccgg
    16441 cgcggcgacg agcgaactcg cgatcttcga cgcgctcgac atcgcggcgg gaccggtcgc
    16501 aaccgtgcat gtccccttcc gcgtgccgtc gggtttccat tgcaatttct acgcggcgga
    16561 ctccatgctt taccggcaag cgttcggtgc agcataggga gagagggcgg tgaaggtcgc
    16621 accatttgaa tgcgtcgccg acgtcgcgcg gcggaacggc cgcgagcggc ccgacaagat
    16681 cctcttccat ttcgagggcc gagcgacaag ctacgccgac ttcaatacga acagcaatcg
    16741 cgccgcaaac gcgctggtcg cactcggcct gaagcctgac gaccgcgtcg cctatctcgg
    16801 caagaacagc gacctctatt tcgaactgct gatgggggtc gccaaggccg gcggggtgat
    16861 gaccccggtc aactggcgcc tcgcggagcc cgagatcgcg tggatcatcg acaattgcgg
    16921 tgcgcgcatc ctgtgcgtcg ggcccgagtt caccgagctg gttgcggcga accgcacggc
    16981 cttcccgggg gtcgaacatg tgatcccggt cgagggcgcc gcgaacggct ttcccgacta
    17041 tcgcggctgg cgcgacacgt tcgccgatac cgaccccgac gtgcctcgcg gcgaaggcga
    17101 cgcggtcatc cagatgtata cctcgggcac caccggcaag cccaagggcg cggtactgac
    17161 caacgggtcg gtgcttcgct cgaaactcga ccgcgacccc gcgctgcaag ccgactggga
    17221 acgttgggac gcggatgatg tcggcctcgt cgcgatgccc tgctttcaca tcggcggcac
    17281 cggctacggc atcaccgtga tgaccaacgg cgcgaccggc gtgatcacgc gcgagttcga
    17341 cccaggcggc gtgctcgacc tgatcgaggc ttatggcata tcgaaaatct tcatggtccc
    17401 cgcggcgatg cagatcatcg tgggtcagcc gcgcgcgcgc gaagtcgatt tctcgcgcct
    17461 ccgtcacatc gcctacggcg cctcgccgat cccgctcgac ctgctgcgcc agtgcatcga
    17521 cgtcttccag tgcggcttcg tccagatgta cggcatgacc gaaacatcgg gcacgatcgc
    17581 cgcgctgccg cccgaggatc atgatcccgc gggcaatgag cggatgcggt cggtgggcaa
    17641 acctttgcgc ggagtcgaga tgcggatcat cgatgccgag ggaaatcccc tgcccccccg
    17701 caaaatcggc gagatcgcga tccgcacgca ggcgaatatg cgcgaatatt ggggacggcc
    17761 ggaggcgacg gcggagacca tcgacgcgag cggctggctg cgcaccggcg atgcgggcta
    17821 caccgacgag gacggctatt tctacctcca cgaccgggtg aaggacatga tcatctcggg
    17881 cggcgagaat gtttatccgg ccgaggtcga gaatgcgatc tacggacacc ccgccatcgc
    17941 cgacgtcgcg gtggtcggcg tccccgacga caaatggggc gaggccgtga aggcgtgcgt
    18001 cgtgctgcgc gaaggcgcga gcgtcgaggc cggcgagatc atcggctggg cgcgcgagcg
    18061 gatcgccggc tataaatgcc cgaagtcggt ggacttcatc cccacactgc cgcgcaaccc
    18121 gtcaggcaag atcctgcgcc gcgaactccg cgccccctat tgggaagggc gcgagcggca
    18181 gatcagctag cctgcagctt cgccatcgcg gccccaaccg ccgcagcaat atcggcttct
    18241 tgcgggttgc cgcgcagcgc gagcccgcca ttgggctgca aattctcgcc cgtcacgaac
    18301 gcctcgtcgc tcgcgagcca gaggcatgcg ttggcgacat cctgagccgt gttgagccgc
    18361 cccagcggat agcgcggcag aaaggcgtcg accaatcccg gcgttgcaaa cgcaccctcg
    18421 gtcatcggcg attcggtgaa cgccggcgag acaatgttcg cacggatgcc cgcggcgccg
    18481 aaatcattgg caacgcagcg gatcagcgcc tccgatcccg tcttggttcc gatataggca
    18541 gcatggttgc tgatcgggga tcgcgtcgtc gccgaggaaa tctggataat cgacccgccg
    18601 gtcggctcgt tggccagcat cgcgccgacg aacgcttgca gaaaatgatg caccccggtg
    18661 aactgcagcg cgacaatcgc cgcgagttcc tcctcggtca catcgagcag cggcttgagc
    18721 agcccccagc cgctcgcgtt catcgcgata tcgaccccgc ccatcttcgc cttcgccgca
    18781 tcggcgagcg cgaagaccga cgccttgtcg gtgatgtcgc agagcgcggc atggccgccg
    18841 atctcggccg cgagcgcatc gagcggttcc gccttgcgcc ccgcgaccat caccgtcgcg
    18901 ccctcgcgcg cgaaatgccg cgcggtcacc tgcgccatat ttcccgtcga tgccgcaccg
    18961 atgatgacgg cgcgcttgcc tgccagcctg cccatatgcc tctcctcaga gtttcaagag
    19021 ctgtttgccg cggttctgcc cggcgaacag acgtttcagc gtgtcgggcg cattctcgaa
    19081 cccgtgctgc acatcctcgc ggtagttgag ccgcccatcg agcacgaagc cttcgaggcg
    19141 cttgcggatt tcgggaaatt cgctcgccca gtcgagcacg atgaagcccg ccatcgaacc
    19201 gcggcgaaag atcaggttga aataattctg cggtcccgcg ggcagcgtgc ccatctcgta
    19261 gcgactgatc ccgccgcaca ccgcgacgcg cgcaccggtc gcgatttcgc cgagcatgtc
    19321 gttaaggatc gcgccgccaa cattgtcgaa gatgacgttc acgccgcccg ggcagtgttc
    19381 cttgatctgc gcccgtacgt tgcccgcctt gtaatcgatc gcggcatcat accccgcctc
    19441 ttcgacgagc cagcggcact tgtcctcgcc ccccgcgatc ccgaccgcac ggcatccggc
    19501 gatcttcgcg agctggccga cgaccgatcc cgtcgcgccc gcagcgcccg acaccagcac
    19561 ggtatcaccc gcgaccggcc gcccgacctt gaacagccca caataagcgg tgacgccggt
    19621 ggtccctagc accgacagca tcgcggtcgg cggcagttcg gtctcgatct tcaccagccc
    19681 ctcccccgtc gagacatgcc attcggtcca gccggtcgat cccatgacca gatcgcccgt
    19741 cgcaaagccc ggatggttgc tctcgacgac ttcgctgatc ccgctgccgc gcatcacgtc
    19801 gccgatcgcc atcggcgcga catagtcggc gatattctcc atccacccct tctgcgccgg
    19861 atcgaagccg agatagagcg tcttgagcag caattcgccc gcgcccggcg cgccgatccc
    19921 ggtctcgacg agccggaaat cgccatcctc gatcccgcgc ccgcgcgggt gtccgttgag
    19981 caaccattgg cgcgatgtcg gcatcgatac tctccctgtt ccggcaccga aattgcgccc
    20041 gcgttcggca gccagccacc gccaaaagtg ccagtcgatt ggaccaccgg caggggtagg
    20101 atgtgatcag ggagagagac gatgcccgag gtccgccgca gtttctgccg cttctgccac
    20161 gccaattgcg cgatgctcgt caccatcgac gaagggcgcg tgaccaaggt ccagggcgac
    20221 cccgacgatc cggtattcgg cggttatacc tgcatcaagg gccgccagct tgccgaggcg
    20281 caccacggtc cgcagcggtt gaccgtctcg cagaagcgcg tcgacggagc gttccaagat
    20341 atcgcgatgg acgacgcgct cgacgaaatc ggtgcaaaac tggcggcgat catcgccgag
    20401 cacggcccac atgccgtcgc catctatggc ggtacttacg cgttccagaa cagcgccggg
    20461 gtcggtagcg cgatgtcgtt cgcgcagggg ctcggcacgc gcaacatcta tacctcggtg
    20521 acgctcgacc agccggcgaa agtctatacg acgatgcgct acggcagctg gatgggcggg
    20581 atgcacacat tcgccgaggc cgacgtcgcc tttttcatcg gcaacaatcc gatcgtctcg
    20641 cactatggcc cgccgggcgg cctaccgccc ttctcgccct cgcgccgcct gcgcgacgcg
    20701 ctcgaaggcg ggatcaagct gatcgtcgcc gacccgcgcc agagcgacgt cgcggcgctc
    20761 gcgcatatcc atctgcccgt ccgcccgggc gaggaccccg cgctgctcgc ggggatcatc
    20821 aacgtcatcc tgtccgaagg gcttttcgac aaggcgttcg tccagcgcta tgtcgacggg
    20881 ctcgacgaat tacaacgcgc ggtcgcgggc ttcactcccg atgttgccgc cgcacgcgcg
    20941 ggggtcgagg aaagccagct cgtcgccgcg gcgcggatgt tcgcgaacgg caccaagggg
    21001 gtcgtctcga ccggcaccgg ccccgagatg gcgggcaacg gcacgctcac ccaatatctc
    21061 gtctcgtcgc tcaacatcat ctgcgggcgc ttctgccgcg agggcgagaa aagctcgatc
    21121 ccccgcgttt tcacccccgt caccccgcgc cgcgcgcagg tcgcgccgcc gatggcgctc
    21181 tatggcgagg gcttccccgc ttcgcgcatc cgcggcctca cccatctcgg gatggaaatg
    21241 ccgtgcaacg tcctcgccga cgagatgctg acccccggcg agggccaggt gcgcgcgctg
    21301 atcgtgatcg gcggcaatcc gctggtcgcc ttccccgatc aggacaagat gacgcgggcg
    21361 atcgacggtc tcgacctgct cgtctgcgtc gacgtcaacg ccgagtccgc gaccgcgaag
    21421 cgcgccgact atatcctcgc gcccaagatg tgcctcgaac gcgaggatat cagcaatctc
    21481 agcgaatggt ggtacgagat tccctatgcc cgctataccg aggcgatgat cgaagcgccc
    21541 gaagggctgc tcgaggagtg ggagatgctc tgggaactcg cgcaccgcct cggcaccggc
    21601 atgccgctcg cgggcggcgc ctgcccgatg gacgaacgcc ccacgaaaga ggtcttcctc
    21661 gacctgatgg ttgcgggctg ctcggtcgca ccaagcgtcg tacgcgcgga taccgtcgac
    21721 ggcaaagcgg tgatctatgc cgacctgcat ccggtcgtcg aggcgggcga tcccgacgca
    21781 ccgggacgct tcgacttgac ggcgggcgcg atgcccggcc agctcatcgc ctatgccgac
    21841 gcagtccagc cgacccccga gtttccttgg cgtatggtct cacgccgcag ccggcaccgc
    21901 ttcaactcga ccgggcagaa tctgcccgcg ttgcgcgcca agcgcacgac caaccccgcc
    21961 ttcctgcatc ccgacgacct cgcgctggtc ccctgcgccg acggcgatac cgtccgcatc
    22021 cgctcggccg tgggcgaagt cgtcgcggtc gcgcgggcgg cggacaatat gcgccccggc
    22081 gtggtgtcga tggcgcacgc cttcggtggc cccgacatcg gtcccgacgg cgtccacgag
    22141 catggggctt cgaccaaccg cctcgtcagc gagagcgtcg cctacgaccc catcaccggc
    22201 cagtcgcggc aaagcgcgat cccggtttcc atcatccccg cctgaggagt tttcatgccc
    22261 gacaaccgcc gcttcctgct cgtccgccgc cccaacggcg aacccgtgca ggcggatttc
    22321 tccctcatca ccgaacccac ccccgagctg gccgacggcc agttcctgat ccgcaaccat
    22381 tatgcctcgc tcgaccctgc gatccgcggc tggatggacg atgcgccaag ctatatgccg
    22441 ccgatcccgc tcggcgatcc ggtgcgcgcg tcgacgatcg gcatcgtcga ggcgagcaag
    22501 gccgacggct tcgcgcccgg ccagtgggtg ctcggcctca acggcatcga ggattattcg
    22561 gtcggcaccg tcggcggttt cactcagccg atcgacgcgc gcctcgtccc gtcggtcacc
    22621 aattacctgt cggtgctcgg cgcggtcggg atgaccgctt acttcggcta tatcgacgtg
    22681 tgcgaaccca agcccgacga cgtcgtgctc gtcaccggcg ctgcgggcgc ggtgggctcg
    22741 ctcgtcgggc agatcgcgaa gatcaagggc gcgagcaagg tcatcggcat cgccggcggg
    22801 cctgagaaat gcgcgaagct gatcgatcgc tacggcttcg acgccgcgat cgactatcgc
    22861 ggcaaggacc tcgcggcgct gaccgacgcg atccgcgccg aagcgcccga cggcgtcgac
    22921 atcatcttcg aaaatgtcgg cggcgacatc ctcgatgcgg ggctgatgaa cctccgccac
    22981 ggcgcgcgca tcgggctctg cggcctgatc agcgaatata attcggtcga gaaggtcggc
    23041 gcgcgcaaca tctggcagtt gatcgtccac cgcgcctcga tccgcgggct gctcgtcgcc
    23101 gactatatcc cgcgcttcgc cgagggcggc gccgaaatgg cgaactggct gcaacaaggc
    23161 aagctcctcg ccgacgagca tgtcgacgaa gggatcgaaa acagctttga cgccttcatg
    23221 cggctcttcg cggggagcaa tcagggcaag atgatcctca aaatcgcatg agtccgccac
    23281 gccgtcttct cgtcctatcg ggcggtcacc cctatgacgc cgggcccttc gccgacctgc
    23341 tcgcaagctt taccggctgg acggtcacgc acctgatcca tcccgaagcc gaaaccggcg
    23401 tcgccgaagg cgcggcgaac aataccgacg cgatcctttt ctacgacatg cccggctatc
    23461 gcttcgaggg cggcaaggcg gcgacacggc cgccctcctt cacctttcgc cgcgcaatca
    23521 ccgattattt cgcgcgcggt ggcggcgcgg tcgcgatgca ccatgcgatc gcgggctggg
    23581 ctgactggcc cgaatgggcc gacatgctcg gcgggcgctt cctctatgca ccgggcgaag
    23641 ccaatggcga gaaccatctc gactcgggct atcgccacga cgtccgttac gatgcggtcg
    23701 tgctcgatcc cgggcatccg gtgaccgcgg gcctgccgcc gcgctttccg ctctgcgacg
    23761 agctgtatct ggcgccggtg tggggcgcag tcgccccgct gctccgcgcc gaccatgctt
    23821 tcgttcgcaa caatttctat tcggcggcgc aggcggtcgc gggacggatg ttcagcaacg
    23881 ccggctggga ccatcgcgcc gaaagcgacc tgatcgcatg ggaaaaaccc gtcggcgcgg
    23941 ggcgtctcgt ctatctccag ccgggcgacg gatccgccgc ttacgccgat ccgaaccttc
    24001 gccgcttcct cgccaacgcg ctcacgcatg tcgcggccgc ctaacgaaaa tgtgcatcgc
    24061 gccgccggag cggctgcata tagttgcgcg tataacagga agatcgagga aagaggcgcg
    24121 tgaacgacat cgccgcaagc agttttttcg acccgcaggt gatcgcggat ccgttcgatt
    24181 attatcgcgc ctggatgccc aagagccccg tcgtgcagct ttccgagggc atgttcctcg
    24241 tcctctccta tgatctttgt tcgcaggcga ccggcgaggt cgagacattc tccaacaatt
    24301 tccagggcac gctgtcgggc gcgatggccg aggacggcga tgtcgcggcg atcctcgccg
    24361 aaggatggcc gcaggtcgat acgctgctga ccgccgatcc gccgacgcac acgcgcttcc
    24421 gcaagctcgt caacctcgcc ttctcgatga agcgcgtcgc cgcaatcgaa gaggatatgc
    24481 gcgacgtcgt gatcgacctg atcgagaaga tggcggcaaa aggcgaagcc gacttcgtcc
    24541 gcgatttcgg cattccgctt cccgtcgcga tgattgcgag ccagatcggc atggagggcg
    24601 acctcgaccg cgtgaagcgc tggtccgacg ccttcgtcga ccggctcggc cggatgatcc
    24661 cgaaggaacg cgagctggaa tgtgcgcgcg aggtcgtcga gttccagcat tatgtaaagg
    24721 cgaagatcga ccagcgccgg gccaatcgca ccgacgatct gctctccgac ctcgtctatg
    24781 ccgaggtcga cggcgaacgg ccgctcgagg atgcggaaat cctttcgatc atgcagcagc
    24841 taatggtcgc ggggaatgag acgacgacct cggcgctcgc gggcgggctg ctccagctga
    24901 tcgggaatcc cgaccagatg gcgaaggcgg tcgccgccgc cgacgaagga aacgagcgcg
    24961 cgatcatcaa cctcgtcgag gaagtgctgc gcaccgaaag cccgacggcg ggcatgtggc
    25021 gcatggtgct gaaggacgcc gaactcggcg gtgtgaaaat cccgaagggc gcgatggtgc
    25081 agctgcgcta cgccgccgcg aaccgcgacc ccgccaaata tcccgacccc gatcgcttcg
    25141 acatcgaacg cgccaacgcg cgcagccatc tcgccttcgg caagggcatc catatgtgcg
    25201 tcggcaatat gctgagccgc aaggaaatgg ccgtcgccta ttccgagttg ctgaagcgcc
    25261 tcaccgactt tcgcgtcgcg gacgggcaca cgccgagctg gccgcccaac atgctgctgc
    25321 gcgggctcac cgccttgccg atcacgttcg agcgccgcgc atgaatttcg acctcgacga
    25381 agaccagcag ctgacgcgcg acatgatcgg ccgctttctg gggccggttg acatcgcggc
    25441 gcggcacgcg atgcgggggg ccgaaggcgg ctattcgcgc gcgcgttggc aggagattgc
    25501 cgaccttggc ctgctggcgc tcgccgcgcc agaggcgatg ggcggcatcg gcggaacgat
    25561 ggtcgacctc gcgctcgtcg ccgaggcgct cggaaagggg atcgcgatcg acccttggct
    25621 agaaaatggt gtgttgccga tccggttggc cgcggcagct ggcgatgaca cactggttgg
    25681 cgaactggtc acgggggaaa agttcgccgc ggtcgctttc gccgagccgg ggcgccgcta
    25741 tgaaaccgaa gctctcagta ccaaggccga aggcgacaag atcaccggcg cgaaggcctt
    25801 cgtcctcggc gccccgctcg ccgatgcgct gctggtcacg gttgctggca accgcctcgc
    25861 aaaagccaag cgcaatgcag caggcgtcgt ggagcaagcc tatcccgtca tcgacggttc
    25921 gaatgccgcg acgatcgatc tgcatcgcac tcccgccgac ctgttcgatt tgcccgcggc
    25981 gaaattcccg cgcgtgatcg gcgatgtccg cctgctcgcc gccgccgaga tgctcgggct
    26041 cgcgcagacg atgttcgacg acacgctcgc ctacgtcggc gagcgccagc agttcggcgc
    26101 cgcgatcggt agctttcagg cgatccagca ccggctcgtc gaatgctatg ctgcactcga
    26161 gcaggcgcgc tcgatggtgc tgcgcaccgc gatgctcgac ccgaccaccg acgatgcgaa
    26221 ttggccgcgt ctttccgcag gggcgaaagc ctttgtcggc gaggcggcga cgcggatcgg
    26281 gctggaggcg gtgcagatgc acggcggcat gggccagacc gacgaactcg cgatcggcca
    26341 tgcgctaaag cgcgtgatgc tgctcgacaa atttttcggc gatcaggatc attgcctgcg
    26401 cagctttgcg agggcggcat gagcgcgctt gccccgatcg cacccggcct gtggaccgac
    26461 gaggccgaac cgcggctgat cggcgcgcgc cgcgaggacg gcgagatcgt cttcccggtt
    26521 ccgtcgggcg acgccgccgc gctggtcgaa cccgtcgcgc tgtcgcgcaa gggtacgctc
    26581 tggtcgtgga cgacgcaggg cttcgaaccc aaggaaccgt acagcggccc gcagcccttc
    26641 cagcccttcc tcatcggcta tgtcgaactg cccggcgaag tgatcgtcga gacgcggatc
    26701 gtcgatgcct ccgccgccga cctgatcatc gggatgccga tggaattcgc cgtcgtgccc
    26761 tttgacgacg cgcgctcgac ctacgccttt cgcccggagc gccagccatg aacgacgacg
    26821 tctatatcat cggcgcgggc atccacccct tcggccgcac cgacggccgt tcgggacgcg
    26881 atcagggcgt ttatgcggtg cgcgaagcac tgggcgacgc gggcatcgaa tggggcgatg
    26941 tcgaattcgc ctatggcgga tcggcggcgg cggggtcggc cgacatcatg gtcaacgagc
    27001 tcgggctgac cagcgtcccc ttcatcaacg tcgcgaacgg ctgcgcgacc gggggcagcg
    27061 cgctgaccgc cgcgcgcaac gcgatcgctg cgggggcatg cgacatcgcg ctcgcggtcg
    27121 gtttcgacaa gcatccgcgc ggcgccttca acgccaagcc gtccgactat ggcttgccgg
    27181 agtggtatgg cgaaacgggc atgatgctga cgacgcaatt cttcgcgctc aagatccaac
    27241 gctatatggc gctccacggg atcagccggc ggacgctcgg ccttgtggcc gaaaaggcct
    27301 tccgcaacgg cacgctgtgc gatcatgctt ggcggcgttc gccggtcgat ctcgacacga
    27361 tattgaatgc gccgatggtc aatgatccgc tgaccaaata catgttctgc tcgcccgccg
    27421 agggcgccgt cgcgctgatc ctcgccagcg gcaggaaggt gcgcgaattg ggcgccgacg
    27481 cggtgcgcat cgccagcgtc gctttccgca cccgccccga aggatcgttc gaggtcttcg
    27541 ccccgtcgat cagcgtgggc ggcggcggca agccgacgca gattgccagc aaggccgcct
    27601 tcgaaatggc ggggatcggc cccgaagatg tctcggtcgc gcaattgcag gacaccgaaa
    27661 gcggcgccga aatcatgcac atggccgaaa acggcttctg cgccgacggc gaacaggaac
    27721 aatggctcgc cgaaggctgg agcgagatcc gcggcaggat gccggtgaac accgacggcg
    27781 gctgcctcgc gtgcggcgaa ccgatcggcg cctcgggcct caggcaagtt tacgaaaata
    27841 ccgtgcagct gcgcggccgc ggtggcgcgc ggcaagtgcc gaacgacccc aaggtcggtt
    27901 actctcatgt ctatggagcg cccggcctgt cggcggtggc gatcctcgaa cgctagcgga
    27961 acgcatggat ctggacctca ccaaagacga actcatattc cgcgaggagg tgcgcgattt
    28021 cctccgcacc tcgcttcccg actatgtccg tgacggcgcg cgccgcaccc ccggcgtctt
    28081 tgtcgaaccc gacatcggcc tcgtctggca ccgcatcctc aatgacaagg gctgggtcgc
    28141 ctatcactgg cccgaggatt gcggcggcac cggctggacg ccgatgcagc gctatatctt
    28201 cgaaaaggaa tgcgcgatcg cgggcgcgcc ggcgctgtcg gtgctcggtc tccgcctcgt
    28261 cggccccgtc atctgcgcct ttggcacaca ggagcaaaag gaccgcttcc tgccagcgat
    28321 ccgtgccggg accgattatt ggtgccaggg ctattccgaa ccgcaaagcg gctccgacct
    28381 cgcctcgctc cgcacccgcg cgcggcgcga cggcgacgaa tatgtcgtcg acgggtcgaa
    28441 gatctggacc acccacgcgc accacgcgaa ctggatcttc tgcctcgtcc gcaccgacgg
    28501 cgaggtgaag aaacaggcgg ggatcagctt catcctcgtc ccgatggacc agcccggcgt
    28561 caccgtgagg ccgatcatca ccatggcggg cgaccatgag gtcaaccagg tctttctcga
    28621 cggagcgcgc acgtcggtcg acaaccgtat cggcgaagaa ggccaaggct ggactatcgc
    28681 caaattcctg ctcgagaacg aacgcggcgg gtcgttccac gctccccgcc tgatccgcgc
    28741 gatcgaccat ctggagcagc tcgcgggcgt cgcgccaagc gggcacaatg gtgcgctcgg
    28801 cggcgacatc cagatcgcga cgcggatcgc aaagctccgc ctcgaagccg aagcgctcga
    28861 aaccaccgaa ctgcgcattc tcgccgacat cgcaaagggc cgccccgccg ggccgcagac
    28921 ctcactcgtc aaactgctct cgtccgaaat ccatcagcgg atagacggcc tcgcgatgga
    28981 cctgcacggc tacgacgggc tgcaattgcc caccgtccgc ccgctctacg gcaatgaagc
    29041 gccgccagcg attggcgacg aggacgcgca ggtcgcggca gcccgctatc tcaacagccg
    29101 cgcctggacc gtcttcggcg gttcgagcga agtccagaaa aacatcatag caaagacggt
    29161 gctccgctta tgacacatgc attgcgactt gccgatcagt gtgaaatagt cccacttatg
    29221 ggagaacagg cagaaaaact gccgatcgac gggttcgcac ccccaatgga tgccaggacg
    29281 cttgtcgatg cggtcacggt ccgtagcgtg gaggatatcc atgacgcggc ggttgttctg
    29341 cgcgactatg ccgccgctta tggcctgcgc gccgcgctct gcgacgatat cgcgtcgaag
    29401 gaaacgatgg tcgatgccga cggcgccgtc ctcgctgccg acctgttcgg ctggctcggc
    29461 gatggcgagc gatggtggga ggatcacagg ctggcgctcc attcgccgct gccacgttcg
    29521 tgccggtacg agagcgagcc tttctggtgc aatgccgaag ggttccgcac cgcgaacccc
    29581 aacccctatc tcgatgatat cgacctctcg cgctattttt cggcgcacga gcggtacaag
    29641 gcggcgatcg tagttccggt ccatctcccg ttcgggcaga tctcagcgaa cagctttcag
    29701 ccttcggatc ccgcgatcac cgatctgtcg gacatgttcg cggcgattgg cgatacgctg
    29761 gcgctcgcaa cgcggcgttt catcgcgggc tatgcctcgg cgatgcggac caagcggcgc
    29821 atcccgtcgg actgcgaact atcgaagcgc gaggtcgaat gcctgcgctg ggccgcgatc
    29881 ggcaagaccg accgcgagat cggcatgatc atctcgctga gccacgccac cgtgcgttat
    29941 cacgtccacc gcgcgggcga gaaactgaac tcggtcaacc gggcgcagac gatcttcaag
    30001 gccgggcaac tggggtatct gggcgcgaac agctaaccca gcgacctgag gggttcgctg
    30061 ccgtcccagt ccttcgccgc gtcgcgcatc accgcgaaca attcgcgcgt gcccgcctgc
    30121 ggttcgagca gttcgaccag cgtcccggga cctgttcccg gatcggcata gatgaccccg
    30181 cccgtgtcgc cgacgggtgc ctcgaaaata atctccgccc caacctcatc gcagcgcacc
    30241 cgcgcctcgg cgatcgactc caccagcacg cacatatggt gcagcccctc gcccaccgca
    30301 tactccccgc gatagagcga cttcgcgtca ttctcgggcc ggatcagctc gatctgcatc
    30361 tcgccccaat agccgagcgc catggtgaag accggatcgc tcggcgcacc gcgatagcgt
    30421 ccgccgggga gcgacacgtt cggcagcagg aagaacggcc ccgcgcccat caccccggtc
    30481 cagtgccgga tcgccgcgtc gaaatccgac ggcacaaacg cgagctgcat gatgtcgccg
    30541 agcgacgcga gcgatttggg tccgggcatc cgtttccccc tcaaaagcga tagcgcacaa
    30601 gcgccgggct ttgtgcgata agcctttcga gacccggagt catactgggc aaattgtgag
    30661 ggatgccatg gccgagtccg atccgaagat cgcgccgcag gactggttcg ccgtccgcaa
    30721 gcgcaatgcg cgcgcgggcc tccgcaagct ggtcgcgatc gtcgccgaaa accgcaccga
    30781 ccgtgcggag gcgccgctgc cgctccataa atccatctat accggcgagg tgcgccatgc
    30841 cgccgagatg gagcatatct tccggaacga accgatcgtc gcagggcttt cgggcgatat
    30901 cccgaaaacg ggcgacacgc tcgtcttcga ctcggtcggc ccgtcgatcc tcgtcacacg
    30961 cggcaaggac ggcgtcgcgc gggccttcct caacatgtgc atgcatcgcg gcgccaagct
    31021 ggtcgaggaa aaggagccgt ggagcggccc gcgaccgcgg ctcgtctgtc cgttccacgc
    31081 ctggaccttc gacccggcgg gcacccttgt cggccagccc ggcaaggaag gcttcgcgaa
    31141 ctgcgagatc ggcgcccgca acctgatcga actgccctgc gccgaacatc tcggcctgat
    31201 cttcgtgcgc gccaatcccg acggcgaacc gatcgatgcc gcggcgcatc tcggcgactt
    31261 cggaccggta cttgcgcagc tcgaactcca ccgcgccgag ccggtgaaga agggcatatt
    31321 gacggcggat tcgaactgga aattcgcgct cgacacctat ggcgagggct atcatttcaa
    31381 gatgctccac gcctcgacga tcggcggcac gcattacagc gaccgcaacg tctatgaacc
    31441 gatcgggcgc caccaccgcg tcagtttccc cgacctgtcg atcggcaatc tcgtcggcaa
    31501 ggacgagagc gaatggcccg aaaccgatta tggcggggtg cattttctgt tccccaacac
    31561 cgtcatcttc ttcggcgcgg tgacccccgg cgtctatttc acgcaggtat tccgcctgtt
    31621 tcccgacgga gcgggcaaga tgttctgcca gttcgcggtc tacgccccct tcggcgtcga
    31681 gagcgacgag caccgcgcga tgtgcgagat ggcctatgac gcgaccgcga ccgtcgtcca
    31741 gaccgaggat tatcgcgtcg cgagcagcgg ctatgcgaac ctcgtcaccg cccccgacga
    31801 cttccacgtc gtgctcggcg cgaacgagcc ggcgctgcac ggcgtccacc gctcgatcgc
    31861 cgccgcatgc aagatgccgc tcgaccagat cgcctgaccg gagcccaatg atgaacgacc
    31921 tttcgcctcc ccaggaccag aaccgcgacg accgctgccc cggcaccagc tacaccgaca
    31981 tgctcaacgc cgacacgcgg cgcgttcccg actatctgtt cgccgaatcc aatcaggaac
    32041 tcggcgacga gccgatcgcg atcgagcgtt atgtcagcga ggactgggcg aagcgcgagc
    32101 gcgagacgat gtggccgaac atctggcagt tcgccgcgcg cgaagaggat atgccgaatc
    32161 ccggcgacac cgtcgtctat gacatcaacg aaaagagcat tcttcttgtc cgccagaagg
    32221 atgggtcggt caaagccttc tacaacgtct gcctccaccg cggccgcaag ctcaggaccg
    32281 agagcggtcc cgcggtcaat ctccgctgcc cgttccacgg cttttcgtgg catcacgatg
    32341 gccgtttcaa ggaagcgccc tgcgcatggg acttcaaaca tctcgacgac aaggacatga
    32401 gcctgcccga gttgaaggtc gaccgttggc agggcttcat cctcgtcacc gagaaccacg
    32461 acctgccgcc gttccgcgag tgggtcggcc ccgggatcga gcattatgac aattggcggc
    32521 tcgacgaatg ccataccggc gcgtgggtcg gccgcgtcat ccccgcgaac tggaaggcgg
    32581 tcgccgaagc cttcatggaa gcgtggcaca gcatcgttac gcatccgcag atcctgggct
    32641 tcaccgccga tgcgaacacg cgctacgacc tttacggcga tttcctcaac cgcgcgatca
    32701 cgccggcggg cgcgatgtcg ccgcacatca agggcaagga tcaattctac gtcctgaaat
    32761 cgctcgagga tttcatcggc gcgggcgatt cgagggcgcg gcgcagcacc gcgtccgccg
    32821 acgatttgaa gggtttcgac gccgacgatc cgatgctggc gcgcaaggtg ttggcgcagg
    32881 cgaaccgcga cgccttttcg gcgatgaacg ggcatgatta ttcggacgcg accgacagcg
    32941 agatgctcga caatttcacc tataatgtct ttcccaactg ggcgccgtgg ggcgggctga
    33001 cgccgaacat cgtctatcgc tggcgcccgt ggggcgacgt cgaccattgc ctgatggagg
    33061 tccgcatcct gatgcgcacg ccgaagggcg aaaaaccgcc gcgcgccgca ccgatgcacc
    33121 tgatccccga cgaccagccc ttcgccagcg ccagccactt gatcggcgcc gcgctcgccg
    33181 gcgtgttcga ccaggacatg gaaaacctcc cctatgtaca ggaggggatg aaatcatcgc
    33241 ccaatggggt gatcgagctc ggccattatc aggaaagccg catccggcat ttccaccgga
    33301 cgctcggcaa atatgtgggc ggtgagcttc ccggctgatg gcgcgcaccg tcccgctgct
    33361 atcgctcgcc gcgggcgtgc tgcccgaatt ttcgccggag cagacggtcg cggccgcgat
    33421 cgcgggcggc tggccggcgg ccggcatctg gtacgacccc aaaagctgga cgcccgcaac
    33481 gacgatcgag gtccgcgacc gcgccgccga cgccgggctt acgatcctcg atatcgaggt
    33541 gatctggctg aaacccggcc ccgacgatcc ggcacatttc gccgcgatcg atgcgggcgc
    33601 ggcgatcggc gcgcgcaatg tcctgatcgt cagctcggaa gacgataccg gacgggccgc
    33661 gggcaagctg aaccggctcg ggaaccacgc cgccgcggtg ggcctgcgcg cctgtctgga
    33721 gttcgctgcg ttcacgacgg tcggcgacat agcgagcgcg gtcgccctgc tcgatgccgc
    33781 cgattgcgat aatctcggcc tgctgatcga cccgttacat ctcgcacgga ccggcggctc
    33841 gcctgccgac ctcaattcgg tcgacccgcg gcgctttcct tatgcgcaat tctgcgatgc
    33901 cccggcgcat ggcccgccgc cgagcgacgt tgcggaaatc atccgcgaag cgctcgatct
    33961 gcgcctgatg ccgggcgacg gcgacttgtc actggacgac ctgcttgcgc agttgccgcc
    34021 gacgactccg ctcagcgtcg aactgcggag cgctgccctg cgagacgatc atcccgacgc
    34081 ggccgatcgc gcacgaagcc tgctcgcggc gacgcagcgc tggttcgcgg cctaggtcca
    34141 gtcc
//
LOCUS       DATMSU010000512         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_103306,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000512 DATMSU010000000
VERSION     DATMSU010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103306"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VN034_13705"
     CDS             <1..341
                     /locus_tag="VN034_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61713.1"
                     /translation="LRKGRPVFTYRSLDLKLTEIAADRALRQGPATILLRFERRGESE
                     ALVHIFSDGAEIASGTIKGALPRLSFSPSETFDLGQDSGSGPLADGRTAGALDGRIHT
                     VNVRITPPAR"
     gene            361..2469
                     /locus_tag="VN034_13710"
     CDS             361..2469
                     /locus_tag="VN034_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWV61714.1"
                     /translation="MAFTTIPGADGDHVTYCRVCEALCGMVATVADGKIVKIRPDRDN
                     PHSRGHICVKGPALAEVTYDPDRVTTPLKRVGGPGEFVPVGWDEALDDIAARLSASVD
                     QHGTQSFAMNSGNPPSMGWPSSMGTYLFSRAMECTKLFTPSSEDITSVVLATELIFGT
                     HGFVFPDLAQCDHLLIFGSNPLVSHGSLLIAPRFKEDLVEIAERGRVIVVDPRRSETA
                     RKFEHVSIIPAGDVWLLGAMVRTVLAEGLADLDFIERHCTGFAELQAAIDWITPELAA
                     PRCGIEADDIRTLARDFVAAPRAAAMGRIGICRASYSTLTNYFLHLLNVISGKFHKPG
                     GVGWGHGGTATDAQSKGPGQARHKRHSSRVSGQASVWGTQSSLTFLEEMTTPGDGQIK
                     SLLTVGANPVLSMPGGPRLPEGFAMLDLMVSVDLYMTETSRHAHYILPATTALEREDI
                     NQFFMNHMVRPFAQYAPAVIPPTGEARGEYEILRDLAARMGRADAFGNMTPFEMADGA
                     LQAGIEGRDGLSLAKLKNNPHGLMIERGRWAFDFEKRIGHPDGKIHLWDAIAADEVLR
                     LRAEPAPDPNTLRLINLRKLRSINSWMHNVDKLVRSDEPRLLINPSDAAARHIDDGMA
                     VRLSSQWGAIEVVAEVTDDVREGCVAYPHGWGHRGAWRTANAKPGANLNEITPATSDQ
                     AEQVSGMSWLEGFPVEISAA"
     gene            2466..3761
                     /locus_tag="VN034_13715"
     CDS             2466..3761
                     /locus_tag="VN034_13715"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61715.1"
                     /translation="MTATPWPSLRRAWYVAGLLCLIAALSYLDRYIIALLADPIIKDM
                     GIDATAIGLLIGLGFGLLYALSGVPVAYLIDRGTRVRIVGVGVIIWSLSTVASGFAPN
                     FEILLATRAGVAIGEAVLVPATVSIVADLFPPEKRALPIGLFMAVSTLMASGAFLIGA
                     YAFELATILSPDLGLSPWRLTLVIVGVPGLILGPLWLLTVAEPDRIEEPGQADQTSVT
                     VAFAYLRRHWRFYLPFYLSFGVSALASYSFIAWTATMLTRSYGQSIADAGAIFGFVGV
                     VAAAVAAVFWPAISAWTIKRDRAELAVLALAVGLGIGHAAVAAFLLTGDIDGAIAVIA
                     VATFGYGAAGGLAVLIIQHIAPPRMRAKITSLYVLVGNLVGLTCGPALSAWMSDTVFT
                     GGDALRQSLSVLALVMLPLTVGLILLAMTAHRRVANEGR"
     gene            complement(3766..>4681)
                     /locus_tag="VN034_13720"
     CDS             complement(3766..>4681)
                     /locus_tag="VN034_13720"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HWV61716.1"
                     /translation="PPKGGWPVMAWAHGTTGIADICAPSARPRGERDSRYIASWLDAG
                     FVVVASDYQGLGTPGIHPYSNYRASSYSVLDSARAIVGDARYGAKNAIVMAGQSQGAG
                     VVVAAAGYAPTYAPGLKLRGVVATGAPNLSREAIESGLASSATDVMVTGAYAMIGYEL
                     TRTHAALDADAVFTDEGRAIEAMVGSACLPDLMKAAAAKELDPSKAFRPGMLKRLWDT
                     DVDLRSFPTLDLAPPLFFGIGAADTAALPMTTLTLVNDLCSRGNVVEAHIYKDKDHSG
                     VVNAAVPDAIRFAKAVLEGRPISATCKA"
BASE COUNT          733 a         1649 c         1498 g          801 t
ORIGIN      
        1 acctgcgcaa gggccgcccg gtcttcacct accgcagcct cgacctcaag ctgaccgaaa
       61 tcgccgccga ccgcgcgctg cgccaggggc ccgcaacgat cctgctccgc ttcgaacggc
      121 gcggcgagag cgaggcgctt gttcacatct tcagcgacgg cgccgaaatc gccagcggca
      181 cgatcaaggg cgccctgccc cgcctctcct tctcacccag cgaaactttc gatttggggc
      241 aggacagcgg atcgggcccg ctcgccgacg gcaggacggc gggcgcactc gacggccgca
      301 tccacacggt caacgtccgc atcaccccgc ccgcgcggtg acacaggaag gaagttgcac
      361 atggccttca ccaccatccc cggcgccgac ggcgaccatg tgacctattg ccgcgtgtgc
      421 gaggcgctgt gcggcatggt cgcgacggtc gcggacggca agatcgtcaa aatccgtccc
      481 gaccgcgaca atccccattc gcgcggccat atctgcgtca aaggtccggc gctggccgag
      541 gtcacctatg accccgaccg cgtcacgaca ccgctgaagc gcgtcggcgg tcccggcgag
      601 ttcgtgcccg tcggctggga cgaggcgctc gacgacatcg ccgcgcgcct gtcggcctcg
      661 gtcgaccagc atggcacgca aagctttgcg atgaacagcg gcaacccgcc gagcatgggc
      721 tggcccagtt cgatgggcac gtacctgttc agccgcgcga tggagtgcac caagctgttc
      781 acgccgtcgt cggaggacat cacctcggtc gtgctcgcga ccgaattgat cttcggcacc
      841 catggctttg tctttcccga cctcgcgcag tgcgaccatc tgctcatctt cggttcgaac
      901 ccgctcgtca gccatggctc cttgctcatc gcaccgcgct tcaaggagga tctggtcgag
      961 atcgccgagc gcggccgcgt catcgtcgtc gacccgcgcc gcagcgagac cgcgcgcaag
     1021 ttcgaacatg tctcgatcat ccccgccggc gacgtctggc tgcttggcgc gatggtgcga
     1081 accgtcctcg ccgaagggct ggccgatctg gatttcatcg aacgccattg caccggcttt
     1141 gccgagcttc aggccgcgat cgactggatc accccagaac tcgccgcccc gcgttgcggg
     1201 atcgaggccg acgacatccg cacgctcgcg cgcgatttcg tcgccgcacc gcgcgccgcc
     1261 gcgatggggc gcatcgggat ctgccgcgcc agctattcga cgctgaccaa ctatttcctc
     1321 catttgctca acgtcatcag cggcaagttc cacaagccgg gcggggtcgg ctggggccat
     1381 ggcggcaccg caacggacgc ccagtcgaag ggacccggtc aggcgcggca caagcgtcac
     1441 agttcgcgcg tcagcgggca agcctcggtc tggggcaccc agtcgagcct gaccttcctc
     1501 gaggaaatga cgacgccggg cgacggccag attaagagcc tgctcaccgt cggcgccaat
     1561 ccggtcctgt cgatgcccgg cggtccgcgt ctgcccgaag gcttcgcgat gctcgacctg
     1621 atggtgtcgg tcgacctcta tatgaccgag acctcgcgcc acgcccatta catcctgccc
     1681 gcgacgaccg cgcttgagcg cgaggacatc aaccagttct tcatgaacca tatggtgcgc
     1741 cccttcgcgc aatatgcccc tgcggtcatc ccgccgacgg gcgaggcgcg cggcgaatat
     1801 gagattttgc gcgaccttgc cgcgcgcatg gggcgcgccg atgcgttcgg caacatgacc
     1861 ccgttcgaga tggccgacgg cgcgctccag gccggcatcg agggtcggga cggcctgtcg
     1921 ctcgccaaat tgaagaacaa tccgcacggg ctgatgatcg agcgcgggcg ctgggcgttc
     1981 gatttcgaga aaaggatcgg ccatcccgac ggcaagatcc acctgtggga cgcgattgcg
     2041 gccgacgagg tcctgcggct tcgcgccgag cccgcgcccg atccaaacac gctgcggttg
     2101 atcaacctgc gcaagctccg gtcgatcaac agctggatgc acaatgtcga caagctcgtg
     2161 cggtccgacg agccgcgtct gctgatcaac ccgtcggatg ccgcagcccg ccatatcgac
     2221 gacgggatgg cggtcaggct ttcgagccaa tggggggcga tcgaggtcgt cgccgaggtg
     2281 accgatgacg tgcgcgaggg gtgcgtcgcc tatccgcacg gctggggcca tcgcggcgct
     2341 tggcgcaccg ccaacgcgaa gccaggcgcg aacctcaacg aaatcacgcc cgcgacgtcc
     2401 gaccaggccg aacaagtatc gggcatgagc tggctcgaag gcttcccggt cgagatttcc
     2461 gcggcatgac cgcgacgccg tggccgtcgc tgcgccgcgc ctggtatgtc gccgggctgc
     2521 tctgcctgat cgcggctttg tcctatctcg accgctatat catcgcgctg ctcgccgatc
     2581 cgatcatcaa ggatatgggc atcgatgcga ccgcgatcgg gctgctgatc gggctcggct
     2641 tcgggctgct ctatgcgctg tcgggcgtgc ccgtcgccta tctgatcgac cgcgggacgc
     2701 gcgtgcgcat cgtcggcgtc ggcgtgatca tctggagcct gtcgaccgtc gcatcgggtt
     2761 tcgcccccaa tttcgagata ttgctggcaa cgcgcgccgg cgtggcgatc ggcgaagcgg
     2821 tgctcgttcc cgcgaccgta tcgatcgtcg ccgacctttt cccgccggaa aagcgcgcat
     2881 tgccgatcgg cctgttcatg gcggtgtcga cgctgatggc gtcgggcgcc ttcctcatcg
     2941 gcgcttacgc gttcgagctg gcgacaatcc tgtcacccga tctcgggctc agcccctggc
     3001 gtttgacgct cgtcatcgtc ggcgtgcccg gcctcatcct cggcccgctc tggctgctga
     3061 ccgtcgccga acccgaccgt atcgaggaac cgggccaggc cgaccagacg agcgtcacgg
     3121 tcgccttcgc ctacctccgc cgccattggc gcttctatct gcccttctat ctcagcttcg
     3181 gcgtgtcggc gctcgcgagc tacagcttca tcgcgtggac cgcgacgatg ctgacgcgca
     3241 gctatgggca gagcatcgcc gatgccggcg cgatcttcgg cttcgtcggt gtcgtcgcgg
     3301 ccgccgtcgc cgccgttttc tggcctgcga tcagcgcctg gacgataaag cgcgaccgtg
     3361 ccgaactggc cgtgctcgca ctcgcagtcg gtctcggcat cggtcatgcg gccgtcgcgg
     3421 ccttccttct gaccggcgac atcgacggcg cgatcgcggt catcgccgtc gcgaccttcg
     3481 gctacggcgc cgccggcggg ctcgcggtgc tcatcatcca gcacatcgcc ccgccgcgca
     3541 tgcgcgccaa gatcacatcg ctttatgtcc tcgtcggcaa cctcgtcggg ctgacctgcg
     3601 gcccggcgct gtcggcttgg atgtccgata cggtgttcac cggcggcgac gcgctgcgcc
     3661 agagcctttc ggtgctcgca ctcgtcatgc tgccgctgac cgtcgggctg atcctgctcg
     3721 cgatgacggc gcaccgccgt gtcgcgaacg aggggcgctg accggctagg ccttgcaggt
     3781 cgccgaaatc ggccggcctt cgagcaccgc tttggcaaaa cggatcgcgt cgggcacggc
     3841 ggcgttcacg acgcccgaat ggtccttgtc cttgtagatg tgcgcctcga cgacattgcc
     3901 gcgactgcag aggtcgttga cgagggtcag cgtcgtcatc ggcaaagccg cggtatccgc
     3961 ggcaccgatc ccgaaaaaga gcggcggcgc gaggtcgagc gtcgggaagg agcgcaggtc
     4021 gacgtcggtg tcccacagcc gcttcagcat ccccggccgg aacgccttcg acggatcgag
     4081 ctctttcgcc gccgcggctt tcatgagatc gggaaggcac gccgagccga ccatcgcttc
     4141 gatcgcccgc ccttcgtcgg tgaacacggc atcggcgtcg agagcggcat gcgtgcgcgt
     4201 cagttcatag ccgatcatcg cataggcgcc ggtgaccatg acatcggtcg cgctcgacgc
     4261 cagcccgctc tcgatcgctt cgcgcgacag gttgggcgcg ccggtcgcga cgacgccgcg
     4321 caatttcagg ccgggcgcat aggtcggagc gtagcccgcc gcggcaacga ccacgcccgc
     4381 tccctgcgac tgacccgcca tgacgatcgc attcttcgca ccatagcgcg cgtcgccgac
     4441 gatcgcgcgg gcgctgtcga gcacgctgta tgaactggcg cgatagttgg aataggggtg
     4501 gatgcccggc gttcccagtc cctgataatc gctcgcgacc acgacaaagc ccgcgtcgag
     4561 ccagctcgcg atatagcggc tgtcgcgttc gccgcgcggc cgcgccgagg gcgcgcagat
     4621 atcggcgata ccggtcgtgc catgcgccca ggccatgacc ggccagccgc ccttcggcgg
     4681 c
//
LOCUS       DATMSU010000513         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_56156, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000513 DATMSU010000000
VERSION     DATMSU010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56156"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VN034_13725"
     CDS             complement(<1..417)
                     /locus_tag="VN034_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61717.1"
                     /translation="MRIKLLVLALLTLAGAGPAAAQVNQSLDIAIPEAPNIVRVGAAD
                     QLVYELHLTNFSSLPLRLDGLSIVGDKGAPLKSYAGDELAKATGLVGPAETDAQIIPP
                     GRRAVLYINAPVPAAFAPRSISHRLTLGKIGGDGASR"
     gene            621..1826
                     /gene="sucC"
                     /locus_tag="VN034_13730"
     CDS             621..1826
                     /gene="sucC"
                     /locus_tag="VN034_13730"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWV61718.1"
                     /translation="MNIHEYQAKELLAKFGVAVPKGIAAMSVEEAVAAAKQLPGPLYV
                     VKSQIHAGGRGKGKFKELGPDAKGGVRLAKSIDEVEAHAKDMLGNTLVTIQTGEAGKQ
                     VNRLYITDGADIKQEFYLALLVDRATSRIAVVASTEGGMDIETVAHDTPEKIHTITID
                     PATGIQPHHGRSVAAALGLSGDLAKQASKVLGGLYAAFLGTDAEQIEINPLAVCEGKD
                     GDELLVLDAKVAFDGNAMFRHKDLAELRDLTEEDPAEVEASEYDLAYIKLDGNIGCMV
                     NGAGLAMSTMDIIKLNGEFPANFLDVGGGASKEKVTAAFKIILKDPAVKGILVNIFGG
                     IMKCDIIAEGIVAAAKEVNLSVPLVVRLEGTNVQQGKDILANSGLPIVAANDLGDAAK
                     KIVAEVRAA"
     gene            1934..2683
                     /locus_tag="VN034_13735"
     CDS             1934..2683
                     /locus_tag="VN034_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWV61719.1"
                     /translation="MKILVPVKRVLDYNVKPRVKSDGTGVDLANVKMSMNPFDEIGVE
                     EAIRLKEKGVATEIIAVSVGPAKAQETLRTALAMGADRAILVQTDDAVEPLALAKIFK
                     AIADAEQPGLVILGKQAIDDDNNQTGQMLAALTGWAQGTFASAVNVEGDSVNVTREVD
                     GGLETVKLKLPAIVTTDLRLNEPRYASLPNIMKAKSKPLDTKSPADYGVDTAPRVKTL
                     KVSEPPVRSAGVKVADVDELVAKLKALGVHS"
     gene            2680..3609
                     /locus_tag="VN034_13740"
     CDS             2680..3609
                     /locus_tag="VN034_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWV61720.1"
                     /translation="MKTLVWVEHDGSAVKDATLAAVTAAAKLGEVHLLVAGSGVDGVA
                     KEAAQIASVQKVHVADNAAFGHNLPENVAPLVADLMGSHDAFVAPATTTGKNIAPRVA
                     ALLDVMQISEILSVEGPKTFTRPIYAGNAIATVESSDAKLVLTVRGTAFEKAATSGGS
                     GTVEAVSAGGDTGISSFVGAEIAKSERPELTSAKIIVSGGRALGSSEKYEEVIVPLAD
                     KLGAALGASRAAVDAGYVPNDYQVGQTGKIVAPQVYFAIGISGAIQHLAGMKDSKTIV
                     AIYKDEDAPIFQVADFGLVADLFNAVPELTGKI"
BASE COUNT          747 a         1232 c         1174 g          613 t
ORIGIN      
        1 tctgctggca ccgtcgccgc cgatcttgcc gagcgtcagc ctgtgcgaga tcgatcgggg
       61 cgcgaaggcg gcggggaccg gcgcgttgat ataaaggacc gcgcggcgcc ccggcggaat
      121 gatttgcgca tcggtctcgg ccggcccgac gagcccggtc gccttggcga gctcgtcgcc
      181 ggcatagctt ttgagcggag cacccttgtc gccgactatc gacagcccat ccagccggag
      241 cggcaggctg gaaaaattgg tgaggtgcag ctcgtaaacg agctgatccg ccgcgccgac
      301 gcggacgata ttgggggcct cgggaatcgc gatatcgagc gactggttca cctgcgccgc
      361 cgccgggccg gcgccggcga gcgtcaacaa ggccaaaaca agaagcttta tccgcatctt
      421 cggctccctc atcccgatcc ccggctccgc ccgattatca gcccgggctc cggacgtcat
      481 ccggaagacg gaccggctgg ccgcaagcct ttgccgcaca caagaatatc tgcggtttcc
      541 gggggtggcg ttgcgggcga acttgtgctt aggcggcgcc gatcctctcc cttacagagt
      601 cgaaaggccc ggtagacctc atgaacatcc acgaatatca ggcgaaagaa ttgctcgcga
      661 aatttggcgt cgccgtgccc aagggcatcg ccgcgatgag cgtcgaagaa gccgtcgccg
      721 cggcgaagca gctccccggg ccgctctatg tcgtgaagtc gcagatccac gccggcggcc
      781 gcggcaaggg caagttcaag gaactcggcc ccgatgcaaa gggcggcgtc cgcctcgcca
      841 agagcatcga cgaagtcgaa gcgcacgcca aggacatgct cggcaacacg ctggtgacga
      901 ttcagaccgg cgaagcgggc aagcaggtca accgcctcta catcaccgac ggcgccgaca
      961 ttaaacagga attctacctc gccttgctcg tcgatcgcgc caccagccgc atcgcggtcg
     1021 tcgcctcgac cgaaggcggc atggacatcg aaaccgtcgc gcacgacacg cccgagaaga
     1081 tccacaccat caccatcgat cccgccaccg ggattcagcc gcaccatggc cgcagcgtcg
     1141 ccgcggcgct cggcctgtcg ggcgatcttg ccaagcaggc atcgaaggtt ctcggggggc
     1201 tttatgccgc gttcctcggc accgatgccg agcagatcga aatcaacccg ctcgccgtct
     1261 gcgaaggcaa ggatggcgac gaactgctcg tcctcgatgc caaggtggcg ttcgacggca
     1321 acgcgatgtt ccgccataag gatctcgccg agctgcgcga cctgaccgag gaagatccgg
     1381 ccgaggtcga ggcgtcggaa tatgatctcg cctacatcaa gctcgacggc aatatcggct
     1441 gcatggtcaa tggcgccgga ctcgcgatgt cgacgatgga catcatcaaa ttgaacggcg
     1501 aattccccgc caacttcctc gacgtcggcg gcggcgcttc gaaggaaaag gtcaccgcgg
     1561 cgttcaagat catcctcaag gatcccgccg taaagggcat cctcgtcaac atcttcggcg
     1621 gcatcatgaa gtgcgacatc atcgccgagg gcatcgtcgc cgcggcgaag gaagtgaacc
     1681 tctcggttcc gctcgtcgtc cgtctcgaag gcaccaatgt ccagcagggt aaggatatcc
     1741 tcgccaattc gggcctgccg atcgttgcgg ccaatgatct gggcgatgcg gcgaagaaaa
     1801 tcgtcgcaga agtccgcgcg gcctgacgcc tcgaccaccc ctccccaccg tgagggggag
     1861 gggtgaggtt gacgtttacg taaaccgcaa ttatggcgca gtgcacaatt tgttgagagg
     1921 agctttgcaa gccatgaaaa tcctcgtccc cgtcaagcgg gtgctcgatt ataacgtcaa
     1981 gccgcgggtc aagtccgacg gcaccggcgt tgacctcgcc aatgtcaaaa tgtcgatgaa
     2041 cccgttcgac gagatcggtg tcgaagaagc gattcgcctg aaggaaaagg gcgtcgcgac
     2101 cgagatcatc gcggtttccg tcggcccggc caaggcgcag gaaacgctgc gcactgcgct
     2161 cgcgatgggc gccgaccgcg cgatcctggt gcagaccgac gatgcggtcg agccgctcgc
     2221 gctcgcaaag atcttcaagg cgatcgccga cgccgaacag ccgggtctcg tgatcctcgg
     2281 caagcaggcg atcgacgacg acaacaacca gaccggccaa atgctcgctg cgctcaccgg
     2341 ctgggcgcag ggcaccttcg ccagcgcggt taacgtcgaa ggcgatagcg tcaatgtgac
     2401 gcgcgaagtc gacggcggcc tcgaaacggt gaaactgaag cttcccgcga tcgtcaccac
     2461 cgaccttcgt ctgaacgagc cgcgctacgc gtcgctgccg aacatcatga aggcgaagtc
     2521 gaagccgctc gataccaagt cgcccgccga ttacggcgtc gacacggcgc cgcgcgtcaa
     2581 gacgctgaag gtttcggaac cgcccgtccg ctcggccggc gtcaaggtcg ccgatgtcga
     2641 tgaactggtt gccaagctta aagcgctggg agtgcattca tgaaaacgct cgtttgggtc
     2701 gaacatgacg gcagcgccgt caaggacgcc acgctcgccg cggtgaccgc cgccgcgaag
     2761 ctcggtgaag tccatctcct cgtcgccggt tcgggcgtcg atggcgtcgc caaggaagcc
     2821 gcgcagatcg ccagcgtcca aaaggtccac gtcgccgaca acgccgcttt cggtcataac
     2881 ctgcccgaaa atgtcgcgcc gctcgtcgcc gacctcatgg ggtcgcacga tgcgttcgtc
     2941 gcgcccgcga cgaccaccgg caagaatatc gcgccgcgcg tcgccgcgct gctcgacgtg
     3001 atgcagattt cggaaatcct gtcggtcgaa ggtccgaaga ccttcacccg cccgatttac
     3061 gccggcaacg cgatcgcgac cgtcgaatcg tcggacgcga agctcgttct caccgttcgc
     3121 ggcactgcct ttgaaaaggc tgcgacctcg ggcggttcgg gcacggtcga agccgtctcg
     3181 gccggcggcg acaccggcat ctcaagcttc gtcggcgccg aaatcgccaa gtcggagcgc
     3241 cccgagctga cctcggccaa gatcatcgtg tcgggcggcc gtgcgctggg ttcgagcgag
     3301 aaatatgaag aagtcatcgt gccgctcgcc gacaaactcg gcgccgcgct cggtgcttcg
     3361 cgcgccgcgg tcgatgcggg ctatgtcccc aacgactatc aggtcggcca gaccggcaag
     3421 atcgtcgcgc cgcaggtcta tttcgcgatc ggcatttcgg gcgcgatcca gcatctcgcc
     3481 ggcatgaaag actcgaagac gatcgtcgcg atctacaagg acgaagacgc cccgatcttc
     3541 caggtcgccg actttggcct tgttgccgat ctcttcaatg ccgttcccga actgaccggc
     3601 aagatctaac tgcctgcccc atggccggaa atcatcccgg agtgcccgga ctgggcgatg
     3661 cgccgttcaa cgccggcggt cgccggtcag cacggccggg acccggccgg gaaaccggtg
     3721 acgacgacga aattggcgcg gccgatgaag atgttcacgg ccgcgc
//
LOCUS       DATMSU010000514         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_179326,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000514 DATMSU010000000
VERSION     DATMSU010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179326"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /gene="hutU"
                     /locus_tag="VN034_13745"
     CDS             <1..1159
                     /gene="hutU"
                     /locus_tag="VN034_13745"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWV61721.1"
                     /translation="LTAGLGGMGGAQPLAAVMAGASCLAIECQPSRIEMRLRTGYLDK
                     QAASIDEAISMIEASHAEGKPVSVGLLGNAAEILPEIVRRGIRPDLLTDQTSAHDPVN
                     GYLPAGWTLDQWFAKRESDPAAVAKAAKASMAVHVQAMLDLQAAGVPTTDYGNNIRQM
                     AKDEGVENAFDFPGFVPAYVRPLFCRGIGPFRWAALSGDPEDIYKTDAKVKELLPDNA
                     HLHNWLDMAREKIQFQGLPARICWVGLGDRHRLGLAFNEMVASGELKAPVVIGRDHLD
                     SGSVASPNRETEAMRDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGYSQHSGM
                     VIVADGTPEAAKRLERVLWNDPGTGVMRHADAGYDIAIECAREKGLDLPSI"
     gene            1226..2389
                     /locus_tag="VN034_13750"
     CDS             1226..2389
                     /locus_tag="VN034_13750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61722.1"
                     /translation="MSEAQPQSHPEIREAVRRLCAAFPGEYWQRLDRDRIYPTEFVRT
                     LTEAGFLSVLIPEEYGGSGLGLGAATAVLEEIHRSGCNGGACHAQMYTMGTLLKHGSD
                     AQKQQYLPAIAHGDLRLQAFGVTEPTAGTDTTRIRTFARKVGGKYVVNGQKIWISRAE
                     HSDLMVLLCRTTPREECAKPSDGMSVLLVDMREAAGNGLTIRPVRTMLNHATTELFFD
                     DLEVPASNLIGEEGKGFRYILSGMNAERILIASECIGDGRFFVDRATAYAKDRSVFGR
                     AIGENQGVQFPIARAYVQIAAAAEMVDKAARLFDAGQDGGTEANMAKMLASEASWYAA
                     DMCVQTHGGFGFAEEYDIERKFRETRLYQVAPISTNLILSHVATHALGLPKSF"
     gene            2389..>2673
                     /locus_tag="VN034_13755"
     CDS             2389..>2673
                     /locus_tag="VN034_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWV61723.1"
                     /translation="MDDYTAWVGRSETREDVATAAPLAGLAALLDHDVSPWVPDTVPP
                     LGHWLYFLPTARQSVIGDDGHPRRDGHGLLPPVALPRRMWAGSRVEFLAPI"
BASE COUNT          451 a          920 c          880 g          422 t
ORIGIN      
        1 gctcaccgcg ggcctcggcg gcatgggcgg cgcgcagccg ctcgcggcgg tgatggcggg
       61 cgcgagctgc ctcgcgatcg aatgccagcc gagccgcatc gaaatgcgcc tgcgcaccgg
      121 ctatctcgac aagcaggcgg caagcatcga cgaggcgatc tcgatgatcg aagccagcca
      181 cgccgagggc aagccggtgt cggtgggtct gctcggcaac gccgccgaaa tcctgcccga
      241 aatcgtccgc cgcggcatcc gccccgatct gctcaccgac cagacctcgg cgcacgatcc
      301 ggtgaacggc tacctccccg cgggctggac gctcgatcaa tggttcgcga agcgcgagag
      361 cgatcccgcc gcggtcgcga aggccgcgaa ggcgtcgatg gcggtgcatg tgcaggcgat
      421 gctcgatctg caagccgcgg gggtgccgac gaccgactat ggcaacaata tccgccagat
      481 ggcgaaggac gagggcgtcg agaacgcctt cgatttcccg ggcttcgtcc ccgcctatgt
      541 ccgcccgctc ttctgccgcg gcatcggccc gttccgctgg gcggcgctgt cgggcgatcc
      601 cgaggatatc tacaagaccg acgccaaggt gaaggagctg ctgcccgaca acgcccacct
      661 ccacaactgg ctcgacatgg cgcgcgagaa gattcaattt cagggcctgc ctgcacgcat
      721 ctgctgggtc ggactcggcg accgccaccg gctcggcctc gcgttcaacg agatggtcgc
      781 gtcgggcgaa ttgaaagcgc ccgttgtgat cggccgcgac catctcgatt cgggctcggt
      841 cgcctcgccg aaccgcgaga ccgaggcgat gcgcgacggg tcggatgcgg tttcggactg
      901 gccgctgctc aacgccctcc tcaacaccgc ctcgggcgcg acctgggtgt cgctgcatca
      961 tggcggcggg gtcggcatgg gctattcgca gcacagcggt atggtgatcg tcgccgacgg
     1021 cacacccgaa gcggcgaagc ggctcgagcg cgtgctgtgg aacgaccccg gcaccggcgt
     1081 gatgcgccac gccgacgcgg gctatgacat cgcgatcgag tgcgcgcgcg aaaagggcct
     1141 cgacctaccg agcatctaga ggcttggcgg gcgatgcttc gcatcctaag gctctccccg
     1201 acgatcaggg gagagccaga cgccgatgtc cgaagcccag ccgcagagcc atcccgaaat
     1261 ccgcgaggcg gtacgccgcc tgtgcgccgc ttttcccggc gaatattggc agaggctcga
     1321 ccgcgaccgc atctatccga ccgagttcgt ccgcacgctg accgaggcgg gtttcctctc
     1381 ggtcctgatc cccgaagaat atggcggctc gggtctcggc ctcggcgccg cgaccgcggt
     1441 gctcgaggaa atccatcgtt cgggctgcaa cggcggcgcg tgccatgcgc agatgtacac
     1501 gatgggcacc ttgctcaagc acgggtcgga cgcgcagaag cagcaatatc tgcccgcgat
     1561 cgcgcacggc gacctccgcc tgcaggcgtt cggcgtcacc gagcccaccg cgggcaccga
     1621 cacgacgcgc atccgcacct tcgcgcgcaa ggtcggcggc aaatatgtcg tcaacgggca
     1681 gaagatctgg atcagccgcg ccgaacattc ggacctgatg gtcctcctct gccgcaccac
     1741 gccgcgcgaa gaatgcgcca agccttcgga cgggatgagc gtgctactcg tcgatatgcg
     1801 cgaggccgcg ggtaacggcc tcacgatccg ccccgtgcgc acgatgctca accatgcgac
     1861 gaccgaactc ttcttcgacg acctcgaagt tcccgcgtcg aatttgatcg gcgaagaggg
     1921 caagggcttt cgctatatcc tgtcgggcat gaatgccgag cgcatcctga tcgcgtccga
     1981 atgcatcggc gacggccgct tcttcgtcga ccgcgcgacc gcctatgcca aggaccgctc
     2041 ggtcttcggc cgcgcgattg gcgagaatca gggcgtgcag tttccgatcg cgcgcgctta
     2101 tgtccagatc gccgccgctg ctgaaatggt cgacaaggcc gcgcgcctgt tcgacgcggg
     2161 gcaggacggc gggaccgagg ccaatatggc gaagatgctc gcgtccgaag cgagctggta
     2221 cgccgccgac atgtgcgtcc agacgcacgg cggcttcggg ttcgccgagg aatatgacat
     2281 cgagcgcaag ttccgcgaaa cgcggctcta tcaggtcgcg ccgatcagca cgaacctgat
     2341 cctgagccat gtcgcgacgc acgcgctcgg cctgcccaag agcttctgat ggacgattat
     2401 accgcctggg tcggccgcag cgaaacgcgt gaggacgtgg cgaccgcggc gccgcttgcc
     2461 ggtctcgccg cgctgctcga ccatgatgtg tcgccgtggg ttccggacac cgtcccgccg
     2521 ctcggccact ggctctattt tctgccgacc gcgcgccagt cggtgatcgg cgacgacggt
     2581 cacccgcgcc gcgacgggca tggcctgctg cctcccgtcg cgcttccccg ccgcatgtgg
     2641 gcgggcagcc gcgtcgaatt cctcgctccg atc
//
LOCUS       DATMSU010000515         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_46965, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000515 DATMSU010000000
VERSION     DATMSU010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46965"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1146)
                     /locus_tag="VN034_13760"
     CDS             complement(178..1146)
                     /locus_tag="VN034_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWV61724.1"
                     /translation="MSGEQPTLLSPRVLLPFALVTLIWGSTWIVIKGQLGVVPPSWSV
                     TYRFAVAAIAMFLFAAMRRERMWIEPRAMAFAAVLGIAQFALNFNFVYRAEQHITSGL
                     VAVLFALLIVPNTLLGRAFLKTPLEGRFLAGAGIAILGVGLMILHEYRAAAVGAGEVL
                     FGTALTLAGVMSASTANVMQGTGIARAQSMVVMIAWAMAFGALADGSYAWITTGPPVI
                     EPTAVYLGGVLYLGIIASAVTFPLYFNVIRAVGPGQAAWSSVLIPIIAMGFSTAFEGY
                     RWAALSVAGGVVALVGLVIAVAKRPERPSVSGNTVSMPVQREEELP"
     gene            complement(1143..2153)
                     /locus_tag="VN034_13765"
     CDS             complement(1143..2153)
                     /locus_tag="VN034_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HWV61725.1"
                     /translation="MTATRFFSDNAATVHPAVMEAMAAANRVDTAYDGDALSQSLDAA
                     FSELFETACEVVWIPTGTAANSIILAHFVRPWQGILCYEEAHIEVDECGAPTFYSGGA
                     KLMPLPGRGAKIDAAALKARIAGIRQDVHQVQPAAVSITNATEYGLSWRAEEIGEISE
                     IAKGKGLKLHMDGARFANAVAFLGCAPADVTWRVGVDALSFGFTKNGAMMAEAIVFFG
                     GSGGAGVRELKKRGGHLLSKGRFVAAQIRAMLKDDLWLANARAANAGAAKLAAACGDR
                     LMYPVEANELFVRMSAEEAAKLRGAGFDFYDWGEGAARLVVSWDQDAEAVAPLAAAIS
                     AL"
BASE COUNT          405 a          842 c          746 g          330 t
ORIGIN      
        1 gcgcgggcgc cgcgaccgcg ggcgacttgg ccagcacgac gaaatcgccg tcgggggcgg
       61 cataggtgcc gggttggcaa gcagtctgcg caagagccgg tgcggccggg agcgcggcga
      121 ggagcaaaag cgtgctggcg aagttcttca tatcgccgta ttattacggc aatacagtta
      181 tggcaactct tcctcgcgct gcacgggcat cgagacggtg ttcccgctga ccgacggccg
      241 ctcgggccgt ttcgcgaccg cgatcaccag cccgacgagc gcgacgacgc cgcccgcgac
      301 cgacagcgcc gcccagcgat agccctcgaa cgcggtcgaa aagcccatgg cgatgatcgg
      361 gatcagcacg ctcgaccagg ccgcctgccc cggcccgacc gcgcggatga cgttgaaata
      421 gagcggaaag gtcaccgcgc tcgcgatgat accgaggtag agcacgccgc ccagataaac
      481 cgcggtgggc tcgatcaccg gcgggccggt ggtgatccag gcgtagctgc cgtcggcgag
      541 cgcgccgaac gccatcgccc aggcgatcat cacgaccatc gactgcgcgc gcgcaatgcc
      601 cgtcccctgc atgacattcg cggtcgatgc gctcatcacg cccgcgagcg tcagcgcggt
      661 gccgaacagg acctcgcccg cccccaccgc cgcggcgcga tattcgtgca ggatcatcag
      721 cccgacgccg aggatcgcga tgcccgcccc ggcgaggaag cgcccttcga gcggcgtctt
      781 cagaaaggcg cgcccgagca aggtgttggg cacgatcagc agcgcgaaca gcaccgcgac
      841 gagccccgag gtgatatgct gctcggcgcg gtagacgaag ttgaaattga gcgcgaactg
      901 cgcgatcccg agcaccgccg caaaggccat cgcgcgcggc tcgatccaca tccgctcgcg
      961 ccgcatcgcg gcaaagagga acatcgcgat cgccgcgacc gcgaaacgat aggtcaccga
     1021 ccagctgggc ggcacgaccc ccagctgccc cttgatgacg atccaggtcg acccccagat
     1081 cagggtgacc agcgcgaaag gaagcaggac gcgcgggctg agcagcgttg gctgctcgcc
     1141 gctcatagcg cgctgatcgc cgccgccagc ggcgccacgg cctcggcatc ctgatcccaa
     1201 ctcacgacga gccgcgccgc gccctcgccc cagtcgtaga agtcgaaccc ggcgccgcgc
     1261 agcttcgcgg cctcttccgc gctcatccgg acgaacagtt cgttcgcctc gaccggatac
     1321 atcagccggt cgccgcacgc cgccgcgagc tttgccgcac ccgcattggc ggcgcgcgcg
     1381 ttggcgagcc acagatcgtc cttcagcatc gcgcgaattt gcgccgcgac gaagcgcccc
     1441 ttgctcagca gatgcccgcc gcgcttcttg agttcgcgca ccccggcgcc gccgctgccg
     1501 ccgaaaaaga cgatcgcctc ggccatcatc gcgccatttt tggtgaagcc gaacgacagc
     1561 gcatcgaccc cgacgcgcca tgtgacgtcg gccggggcgc agccgagaaa agccacggcg
     1621 ttggcgaagc gcgcgccgtc catgtggagc ttcaatccct tgcccttcgc gatctcgctg
     1681 atctcgccga tctcctcggc acgccatgag aggccatatt cggtggcgtt ggtgatgctg
     1741 accgccgccg gctgcacctg atgcacgtcc tgacggattc ccgcgatccg cgctttcagg
     1801 gcggcggcgt cgatcttcgc gccgcgcccc ggcagcggca tcagcttggc gccgcccgaa
     1861 tagaaggtcg gcgcgccgca ttcgtcgacc tcgatatgcg cctcctcata gcaaagaatg
     1921 ccctgccacg gccgcacgaa atgggcgagg atgatgctgt tcgccgcggt cccggtgggg
     1981 atccacacaa cctcgcaagc ggtctcgaaa agctccgaaa aggccgcgtc gagcgactgg
     2041 ctgagcgcgt cgccgtcata ggccgtatcg acccgattgg cggcggccat cgcctccatc
     2101 accgccggat ggacggttgc ggcattgtcg gagaaaaagc gggtcgcggt catgccccgc
     2161 gccttagcgc aggagcgagc cgcttgctaa cccttcattt ctgctgcggt tcgccggacg
     2221 ggtcggcggg atcttcgggc gccgatccac gcgaaaggct ggctccgccc gccacgccaa
     2281 ggccgcccgc catgcccatg ccgcctcccc cgccgccgcc acg
//
LOCUS       DATMSU010000516         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_75172, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000516 DATMSU010000000
VERSION     DATMSU010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75172"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..122
                     /locus_tag="VN034_13770"
     tRNA            48..122
                     /locus_tag="VN034_13770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Val,seq:gac)
     gene            complement(166..957)
                     /locus_tag="VN034_13775"
     CDS             complement(166..957)
                     /locus_tag="VN034_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61726.1"
                     /translation="MTTFRDNLLAGKTAFVAGGTSGINLGIAKRFAELGAKVAVAGRD
                     PDKAARAAAEIGKGALGLSGDVRDYAAIRGVMEQVAGELGPMDIVISGAAGNFLAPVL
                     GMSANAFRTVVDIDLNGTFNVFRGCHDLLNRPGASLIAITAGQAVNASALQAHACAAK
                     AGINQLIRVLALEWGPEVRVNGISPGPIADTEGMKRLAPDAEARQAHYDRIAMKRWGR
                     IEEVAESAVFLCSPAAGYITGTILDCDGGSQIGDASRGDLTKGMA"
     gene            1064..1933
                     /locus_tag="VN034_13780"
     CDS             1064..1933
                     /locus_tag="VN034_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV61727.1"
                     /translation="MASAGPTSNSFISQRLKLHYVDWSNRGAPPLLLIHGGRDHCRNW
                     DWVAEKLRDRYHIIAPDLRGHGDSAWSPDGNYAMDTFVYDLAQLIHQLDLGPLSIVAH
                     SMGGNIALRYTGLYPDNVRKLVAIEGLGPSPKVIAERAQTSYAERFRKWIDDKRQAAG
                     RTPRRYATLEDALARMMGENAYLTEAQARHLTIHGISRNEDGTWSWKFDNYLNVWPAF
                     DMPQDDIASLWGAIACPTLLLYGANSWASNPEKDGRLQHFNTAKVIEFENAGHWLHHD
                     QFDRFMSTLDEFL"
     gene            1980..>2210
                     /locus_tag="VN034_13785"
     CDS             1980..>2210
                     /locus_tag="VN034_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWV61728.1"
                     /translation="MAYTGQISGRQKALSGGGALVAVAAVGLGLASGLDLTVVRRAGE
                     VITAIAIPAPPPPREQAVPAKAASEKASGKASA"
BASE COUNT          397 a          744 c          690 g          379 t
ORIGIN      
        1 tgccaagccg cgcagaggcc tttagggggc gcgtcacccc aagggatggg cgtgtagctc
       61 agtggtagag cactgtgttg acatcgcagg ggtcgcaagt tcaatccttg ccacgcccac
      121 cattaaaaac gccgccgccc catcgggttg gcggcgtttt tgctgtcagg ccatcccctt
      181 cgtcaggtcg ccgcgcgagg cgtcgccgat ctggctgccg ccgtcgcaat cgagaatcgt
      241 gccggtaata tagcccgcgg ccggcgagca caggaacacc gccgattcgg caacctcctc
      301 gatcctgccc cagcgcttca tcgcgatccg gtcataatgc gcctgccgcg cttcggcatc
      361 gggcgcgagg cgcttcatcc cctcggtatc ggcgatcggc cccggcgaga tgccgttcac
      421 gcgcacctcg ggcccccatt cgagcgcgag cacgcgaatc agctggttga tccccgcctt
      481 cgcagcacag gcatgcgcct gcagcgccga ggcattgacc gcctgccccg ccgtgatcgc
      541 gatcagcgac gcgcccgggc ggttgagcag gtcgtggcag ccgcggaaca cgttgaaggt
      601 gccgttgagg tcgatatcga cgacggtacg aaacgcattg gccgacatgc cgagcaccgg
      661 cgcgaggaaa ttgcccgccg cccccgaaat cacgatgtcc atcggcccca gttcgcccgc
      721 gacctgttcc ataacgccgc ggatcgcggc ataatcgcgc acgtcgcccg acaagccgag
      781 cgcgcccttg ccgatctccg ccgcggcgcg cgccgcctta tcgggatcgc gcccggcgac
      841 ggcgaccttt gcgccgagtt cggcaaagcg tttcgcaatg ccgaggttga tgccgcttgt
      901 gccgccggcg acgaaggcgg tcttgccggc cagcagattg tcccggaatg tcgtcatgct
      961 actctccctt cgcttgtctc tatcgttcga tggcaccgat gcggggttga cggatcgaaa
     1021 ccagtcgcct tttgaaactg gaacagacag caaggatgat ccgatggcaa gtgccggccc
     1081 cacttcgaac agcttcatct cgcagcggct gaagttgcat tatgtcgact ggagcaatcg
     1141 cggcgcgccg ccgcttctcc ttatccacgg cggccgcgat cattgccgca attgggactg
     1201 ggtcgccgaa aagctgcgcg atcgctatca tatcatcgct cccgatctgc gtggtcatgg
     1261 cgacagtgca tggtcgcccg acggcaatta cgcgatggac acttttgtct atgacctggc
     1321 gcagttgatc caccaactcg acctcggccc gctctcgatc gtcgcgcatt cgatgggggg
     1381 caatatcgcg ctgcgctata cgggtctcta tcccgacaat gtccgcaagc tcgtcgcgat
     1441 cgaggggctc ggcccctcgc ccaaggtgat cgccgagcgc gcgcagacga gctacgccga
     1501 acgcttccgc aaatggattg acgacaagcg ccaggcagca gggcgcaccc cgcgtcgcta
     1561 tgcgacactc gaggacgcgc tcgcgcgcat gatgggcgag aatgcctatc tgaccgaagc
     1621 gcaggcgcgc cacctgacca tccacggcat cagccgcaac gaggacggaa cttggagctg
     1681 gaagttcgat aattatctga acgtctggcc cgccttcgac atgccgcagg acgatatcgc
     1741 gtcgctatgg ggcgcaatcg cctgccccac cctgctcctc tatggcgcga acagttgggc
     1801 ctcgaatccc gagaaggacg ggcggctcca acatttcaac accgcaaagg tgatcgagtt
     1861 cgaaaatgcg ggtcactggc tgcaccacga ccagttcgac cggttcatgt ccacgctaga
     1921 cgaattcctc taagccggac gccggttgcg tgccgcggcg cgcgatgaga gacggcgcaa
     1981 tggcctacac ggggcagatt tcgggacggc agaaggcctt gtcaggcggg ggcgcgctgg
     2041 ttgccgtcgc cgccgtcggg ctgggccttg ccagcggcct cgacctcacg gtcgtgcggc
     2101 gggccgggga agtcatcaca gcgatcgcga ttcccgctcc cccgcccccg cgcgaacaag
     2161 ccgtgccggc aaaggccgcg agcgagaagg cgagcggcaa ggcatcggcg
//
LOCUS       DATMSU010000517         7821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_132128,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000517 DATMSU010000000
VERSION     DATMSU010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132128"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VN034_13790"
     CDS             <1..751
                     /locus_tag="VN034_13790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015474 - autotransporter activity
                     [Evidence IEA];
                     GO_process: GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HWV61729.1"
                     /translation="WQAQLGLDAVLAGRSDGAALVGGLTAHYGKADSAVTSIFGDGTI
                     DTQGYGVGATMTWYGPKGFYVDGQVKLSWFDSDFESNVLGSLARGNKGDGQAFSLELG
                     KQTPIGRNLGITPQVQMSYAKVDFDRFADPSDAAVSVAKSESLKTRWGLAIDHQTSWK
                     GRSGDTRHTRLYSVVNLSYEWLDGAEADVSETPIVNRDHRLSGELGLGGSYSWGDDRF
                     TLYTEVSGDTAIADFGAGYNLKGTAGFRVRF"
     gene            1074..1232
                     /locus_tag="VN034_13795"
     CDS             1074..1232
                     /locus_tag="VN034_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61730.1"
                     /translation="MPPSASELFAALRILVLIDEERGLFDRGAEEGGYRSEELEKALE
                     VVRAAVAE"
     gene            1313..1588
                     /locus_tag="VN034_13800"
     CDS             1313..1588
                     /locus_tag="VN034_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWV61731.1"
                     /translation="MNHAELSDSVATSLGLSKAEGKKAVDAVFAAIADAAAKGDEVSV
                     NGFGKFKVKELAAREGRNPSTGETIQIAASKKLGFSAAKAVKDRLNG"
     gene            complement(1645..2172)
                     /locus_tag="VN034_13805"
     CDS             complement(1645..2172)
                     /locus_tag="VN034_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HWV61732.1"
                     /translation="MKLFAIYIGGEMAGANIELHDMRFVVAPSITETYDELRRQWWGI
                     PKSLHIDCWAEIDQVDGYDVVLRPEPFAGPERLYYVNLGGYAEGEFLEKHKNVFVVAD
                     SLAKAKARALKSTAGWRELHRDDLYEAEQAISLSESGASQRLHVHLVPATVRKDLAII
                     CDYTPIRVRKQAAAR"
     gene            complement(2169..3839)
                     /locus_tag="VN034_13810"
     CDS             complement(2169..3839)
                     /locus_tag="VN034_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HWV61733.1"
                     /translation="MTSSIAFVGAGPTTIYTLHALLDRNPPPFVLTIFEEQPSAGRGT
                     PYRPGWNDPAMLSNIASIEIPPLEQTLADWLHAQPAERLAALGIDPGAIDERSFYPRL
                     ALGEYFFDQFQALLDRARARGMKVDVRTRCRVVDAVSKPDGMVLTVRARSGAKSEARF
                     DHVVLATGHQWPAEPEVRPGYFLSPWPASALAALAPAKVGIRGSSLSAIDAAVALAVS
                     HGDFAEESDGSLVYVPAAGTEAFHMTMMSRKGLLPEADFFAPLPYEDLAICTPEAMEA
                     LIAADGLLLEPAYALFQQELAAADPDYAAETGLADLALEDFCERYFVDRVDADPFEWA
                     KDNLAEAERNYEIEHTVPWRYAILRMHEVLAQIVPHLDAEEYRRFAKHFKPVFVDDYA
                     TVPHESIRRMLALHRAGKLDVLAIGDDYRIDSHRAEGGAVVQALGERHAFPVFIEATG
                     QRPLQAKAFPFPSLLDQGIVVDAQPGAAPAASRGIAIDDQFHPISAAIPEDQLFCLSL
                     PFLLGRHPFIQGITSSHEMGRVVAEQLAAAIARDLGASPANDNGVIAA"
     gene            complement(4251..4961)
                     /gene="pgl"
                     /locus_tag="VN034_13815"
     CDS             complement(4251..4961)
                     /gene="pgl"
                     /locus_tag="VN034_13815"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWV61734.1"
                     /translation="MSEEIEWWDYDDAAEMAAAVAGDIGFIIDSAIEARGSAVIALAG
                     GKTPIPIYEKLAAAKLDWKRVTIVPGDERMVPLGDPLSNVTVLAKYFLPKGARVMPIV
                     PNATGDYKAAGRSADALMQDLHWPLDLCLLGVGSDGHAASIFTGPDFEEAVNGPKERR
                     ALGVLPDPMPSDAPVARVTLSLAAITTARALMIAVTGDAKRAVIETAIKQGPASPYPI
                     GRALAGTDLPVDIHWAPA"
     gene            complement(5085..5327)
                     /locus_tag="VN034_13820"
     CDS             complement(5085..5327)
                     /locus_tag="VN034_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61735.1"
                     /translation="MPIYFFNLHSGNALEINDKEGTELSDLRAAIAFAVLSARSHAAD
                     DVLRGQFKSADQIEIVDDAGRVLHRVRFDGAIGLCS"
     gene            complement(5458..5925)
                     /locus_tag="VN034_13825"
     CDS             complement(5458..5925)
                     /locus_tag="VN034_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61736.1"
                     /translation="MKSRILTAGLIAAIMIPAAAQAQTREIERDRREAREERQEYRDA
                     QRYGDRDDVREERREYRDARRDYREEVRDWRRDTRYQNYRAPFRYQQFREGSTLRPNY
                     YAPNYRMRYDSRWGIPQARGNLTYVRHYDDLLLVNQRNGRVVKVYRNHFDRRR"
     gene            6228..6890
                     /locus_tag="VN034_13830"
     CDS             6228..6890
                     /locus_tag="VN034_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWV61737.1"
                     /translation="MEKIVNGASLKPRSGKPPRKIVLLLHGFGSSGSDMISLAPAWAD
                     AMPDALFLAPHAPQRCGMMGAGYQWWALSGFAPSALAAGAAAAAPAIDAFIDRKLAQY
                     GLTEADLALVGFSQGTMMALHVGLRRPRAVAAIVGYSGMLAGTLGLGHGALPKPPVLL
                     VHGTADPVVPIAALHMSESELKRLGIDVTTHISYGVAHSVDPVGLRLGRDFVTEAFVH
                     SS"
     gene            7037..7312
                     /locus_tag="VN034_13835"
     CDS             7037..7312
                     /locus_tag="VN034_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61738.1"
                     /translation="MKKLLIGLAATSALALGACQSPAADKVEDQAEAKAEAIDDQADA
                     MPEGPAKEAMEQKADIVEEKGEEKANAMDDNGEIAPSESGVGDTQKK"
     gene            7483..>7821
                     /locus_tag="VN034_13840"
     CDS             7483..>7821
                     /locus_tag="VN034_13840"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HWV61739.1"
                     /translation="MNRLRHLETFARAGWCARGAVYCLLATFALTGAGASDASPQGVF
                     RSVRDMPAGTLLLLLLTAGLALYGAYRLYGAALDSEGKGSDAKGIATRIGYAASGLAH
                     FVLAWAAARLA"
BASE COUNT         1343 a         2466 c         2613 g         1399 t
ORIGIN      
        1 gtggcaggcg caactcgggc tcgacgccgt gttggcaggg cgcagcgacg gcgccgccct
       61 cgtcggcggg ctgaccgcgc attatggcaa ggccgacagc gcggtgacct cgatcttcgg
      121 cgacggaacg atcgacacgc agggctatgg cgtcggcgcg acgatgacct ggtatgggcc
      181 gaagggcttt tacgtcgacg ggcaggtcaa gctcagctgg ttcgacagcg attttgaatc
      241 gaatgtcctc ggatcgcttg ctcgcggcaa caagggcgac ggccaggcat tcagcctcga
      301 actcggcaag caaaccccga tcggccgcaa cctcggcatc acgccgcagg tccagatgag
      361 ttatgccaag gtcgatttcg atcgtttcgc cgatccttcg gatgcggcgg tttcggtcgc
      421 gaagagtgaa agcctgaaga cgcgctgggg cctcgcgatc gatcaccaga cgagctggaa
      481 gggccggtcg ggcgataccc gccacacgcg tctctacagc gtggtgaacc tcagctacga
      541 atggcttgac ggcgccgagg ccgacgtatc ggaaacgccg attgtaaacc gtgaccaccg
      601 cctgtcgggc gaactcggtc tcggtggcag ctatagctgg ggcgacgacc gcttcaccct
      661 ctatactgag gtctcgggcg ataccgcgat cgccgacttc ggcgcgggct ataatctgaa
      721 gggcaccgcc gggttccggg tacggttttg atcgctgttg aatccggcgc ggtggaaagc
      781 aagtgcgcct ttgaaaattt tgtgagggcg tcaggtcaat ttggctctga cgtccgcttc
      841 ttgcggtttg tcgccaaaag ctgactggca gctaacggcc aatcgcccct agaagctgat
      901 cgtggcgttg aacgagttgt agatcgtatc gccgcctgcc actgtctttg gcgcatcgtg
      961 aagctggcct ggctcggaga gcgcgtgtcg gtgctggcgg ggatcgtcga gaccgtctgc
     1021 cgcgaacgca tcgcgcagct tgccgatacc gcacggagcg cgggctgata gcggtgccgc
     1081 cgagcgcctc ggagcttttt gcggcgttgc ggattctcgt gctgatcgac gaggagcgcg
     1141 gcctgttcga tcgcggcgcc gaggagggcg gctatcgctc cgaagagctg gagaaagcgc
     1201 tcgaggtcgt gcgcgccgcc gtcgccgaat gatggtgctg gcgagcgcct gatcgggcga
     1261 ctatagcgcc tgaccgcccg ggatggcccc ggacgtctca ggagatatgt tcatgaacca
     1321 cgccgaactt tcggacagcg ttgcaacctc gctcggcctt tcgaaggccg agggcaagaa
     1381 ggccgtcgac gccgtcttcg ccgcgatcgc cgatgccgcc gccaagggcg acgaagtctc
     1441 ggtgaacggc ttcggcaagt tcaaggtgaa ggaattggcc gcgcgcgaag gccgcaatcc
     1501 ctcgaccggc gagaccatcc agatcgccgc ttcgaagaag ctcggcttct cggccgccaa
     1561 ggcggtcaag gatcgcctca acggataagc aaatcatgtg tcccgccggc tccggccggt
     1621 gggattttgc cctgcgcgca acccctatcg cgcagccgcc tgtttccgga cgcggatcgg
     1681 cgtatagtcg cagatgatgg cgaggtcctt gcggaccgtg gccgggacga ggtggacgtg
     1741 gaggcgctgg ctcgcgccgc tctcgctcag cgagatcgcc tgttccgcct catacaggtc
     1801 gtcgcggtga agctcgcgcc accccgcggt gcttttgagc gcgcgggcct tcgccttcgc
     1861 gaggctgtcg gcgaccacga agacattctt gtgcttctcg agaaactcgc cctcggcata
     1921 acccccaagg ttcacataat agaggcgttc gggtccggcg aagggctcgg ggcgcagcac
     1981 cacgtcatag ccgtcgacct ggtcgatctc ggcccagcag tcgatatgca ggctcttggg
     2041 aatgccccac cactgccggc gcagctcgtc ataggtctcg gtgatcgacg gcgcgacgac
     2101 gaaccgcatg tcgtgcagct cgatattggc gccggccatc tcgccgccga tatagatcgc
     2161 aaacagcttc atgcggcaat caccccattg tcgttcgccg ggctcgcgcc gaggtcgcgg
     2221 gcgatcgcgg cggcgagctg ttcagcgacg acacgtccca tctcgtgaga gctcgtgatg
     2281 ccctggatga acgggtggcg cccgagcaga aaggggaggc tgaggcagaa gagctggtcc
     2341 tccggaatgg ccgccgagat cggatggaac tggtcgtcga tcgcgattcc gcgcgaggcc
     2401 gccggcgccg cgcccggctg cgcatcgacg acgatcccct gatcgagcag cgacgggaag
     2461 gggaaggcct tcgcctggag cggccgctgc cccgttgcct cgatgaagac cgggaaggca
     2521 tggcgttcgc cgagggcctg gaccacggcg ccgccttcgg cgcgatggct gtcgatccgg
     2581 tagtcgtcgc cgatggccag cacatcgagc ttccctgcac ggtgcagcgc gagcatccgc
     2641 ctgatcgatt catgcggaac cgtggcataa tcgtcgacga agacgggttt gaaatgcttg
     2701 gcgaaacgcc gatactcctc ggcgtcgaga tgcggcacga tctgtgcgag gacttcgtgc
     2761 atgcgcagga tcgcatagcg ccacgggacc gtatgctcga tctcgtaatt acgctcggcc
     2821 tccgccagat tatccttcgc ccactcgaac gggtcggcat cgacacggtc gacgaaatag
     2881 cgctcgcaaa aatcctcaag cgcgaggtcg gcgagacccg tttcggcggc atagtccgga
     2941 tccgccgcgg ccaactcctg ctggaacagc gcataggcag gctcgagcag gagcccgtcg
     3001 gcagcaatca gcgcttccat cgcctcgggc gtacagatcg ccagatcctc ataggggagg
     3061 ggtgcgaaga agtcggcctc gggaagcagg ccctttcgcg acatcatcgt catgtggaac
     3121 gcctcggtcc cggccgctgg cacataaaca aggctgccgt cgctttcttc ggcaaagtcc
     3181 ccgtgcgata ccgcgagcgc caccgcggcg tcgatcgcgc ttagcgagct gccccggatg
     3241 ccgaccttcg cgggtgcgag cgctgcaaga gccgacgcgg gccacgggct gaggaaatag
     3301 ccgggacgaa cctcgggttc cgcgggccat tgatgccctg tcgcgagcac gacatgatcg
     3361 aacctcgcct cgctcttggc ccccgatcgg gcgcggaccg tcagcaccat cccgtcgggc
     3421 ttgctcaccg cgtcgacgac ccggcagcgt gtgcgcacat cgaccttcat gccgcgcgcg
     3481 cgggcccggt cgagcagcgc ctggaactgg tcgaagaaat attcgcccag cgcgaggcgc
     3541 ggataaaagg agcgctcgtc gatcgcgccc ggatcgatgc ccagcgcggc gagccgctcc
     3601 gccggctgcg cgtgcaacca gtcggccagc gtctgctcga gcggcggtat ctcgatactc
     3661 gcgatattcg atagcatcgc cgggtcgttc cagccggggc gataaggcgt gccgcgtcct
     3721 gcgctgggct gctcctcgaa gatggtcagc acgaacgggg gcggattacg gtcgaggagg
     3781 gcgtggagcg tatagatggt ggtcggtccg gcgccgacga aagcgatcga tgaggtcatc
     3841 gctatattat gcttggggga gcgaaagggt gcgcgcgatt cgtataaacc tcgatcatgt
     3901 cgcatcgcct gacggaattt gcctggctcc ttgcctgaaa gcgcggcgaa ccagtccgca
     3961 tagggggtgc tctgcaccat ttggcgatta tagtcgggtg ccccatcgtc cgatgccgcg
     4021 atgagcggct gagcatttca tgcacgcacc gggggccaag gggctcgcag cgccgacagc
     4081 atcgccgacg aacaggatcg cgccgccaaa agctacacgc ccgcggccga atgagcgatt
     4141 atgctccgcg gcgttccata gcaccgtcgg ccacggcggg tggcggggag ggggcatggg
     4201 aagggcaggt ggccctcatt gcccgaagtc ggtccgccga ggcgaagcct tcaggcgggc
     4261 gcccaatgga tgtcgaccgg caggtcggtg ccggcgagcg cgcggccgat cggatagggc
     4321 gacgccggcc cctgcttgat cgcggtctcg attacggcgc gtttcgcatc gcccgtcacc
     4381 gcgatcatga gagcgcgggc tgtcgtgatg gcggcgaggc tcagcgtaac gcgcgccacc
     4441 ggggcgtccg acggcatcgg gtccggcagc acgccgaggg cccggcgctc tttggggcca
     4501 ttgacggctt cctcgaagtc ggggcccgtg aagatcgagg cggcatgccc gtcgctcccc
     4561 acgccgagga ggcagagatc gagcggccag tggaggtctt gcatcagcgc gtcggcggat
     4621 cgccccgcag ctttatagtc tccggtcgcg ttcggaacga tcggcatgac gcgggcgccc
     4681 ttggggagga aatatttcgc gaggaccgtc acattgctga gaggatcgcc gagcgggacc
     4741 atgcgctcgt ccccgggaac gatcgtcaca cgcttccagt ccagttttgc ggctgcgagc
     4801 ttctcataga tcggaatggg cgtctttccg ccggcgagtg cgatcaccgc gctaccgcga
     4861 gcctcgatgg cgctgtcgat gatgaagccg atatcgcccg cgaccgccgc cgccatctcg
     4921 gccgcgtcgt catagtccca ccattcgatt tcttcgctca caggatgttc cttttgttgg
     4981 cggcgctgtg cggcgttggg gcgcctggtg gaatgatggc ggacggaccg cgacctagcc
     5041 tataagcgtg cggcatttcc cggcaatccg atgtggatcg atatctaaga acagagcccg
     5101 atcgcaccgt cgaaccggac ccggtgcagt acgcggcccg cgtcatcgac gatctcgatc
     5161 tgatcggccg atttgaactg gccgcgcagc acatcgtctg ccgcgtgcga ccgggcgctc
     5221 agcaccgcga aggcgatagc ggcgcggagg tcgctgagtt cggtcccctc cttgtcgttg
     5281 atttccagcg catttccgct gtggaggtta aaaaagtaaa ttggcatggc tgaccgaacc
     5341 cgcgaggccg tgctgcgttc ccggcgccat tgttctgtct tcgccgccac agaaaaaggg
     5401 ccgcccctgc gggcgaccct tctcggctga cgcattccag cgatcgatcg cgtgccttta
     5461 gcggcggcgg tcgaaatggt tgcgatagac cttcacaacg cggccgttgc gctggttgac
     5521 gagcaacagg tcgtcatagt gacgaacata ggtcagattg ccgcgcgcct gcgggatgcc
     5581 ccagcggctg tcatagcgca tgcgatagtt gggcgcataa tagttcggtc ggagcgtcga
     5641 gccctcgcgg aactgctgat agcggaacgg cgcgcgataa ttctggtagc gcgtgtcgcg
     5701 gcgccagtcg cgcacctcct cgcgataatc gcgacgcgca tcacgatatt cgcggcgctc
     5761 ctcgcgaaca tcgtcgcgat cgccatagcg ctgtgcatcg cgatactcct ggcgctcttc
     5821 gcgcgcttcg cgccggtcac gttcgatctc gcgcgtctgc gcctgggcgg cggccgggat
     5881 catgattgct gcaatcaggc ccgcggttaa tattctgctc ttcatcgtgc cattccttat
     5941 gaaacggatc gaagcaaggt tcgaccctgc gagcggtgta cgcacccgcg aggcaatcag
     6001 gtctgaatgg actgttcaca tggggtatag cgacagcgct gcgtgccggg acgggtaggg
     6061 gtgcctgctt ctgggcacgc cagcggccaa gtgccgcgcg aagcaatgtt cgcaagcttt
     6121 gacttaacgc gtgcgcggct ggaattcatt ggccaggatt tgcgctttcg cgcatgcaca
     6181 gagcagcgcg ccatattgtg tcgcggtgag ggatgagagc ggtatctgtg gagaagattg
     6241 tcaacggcgc cagcctgaag ccgcgcagcg gaaagccgcc aaggaagatc gtcctgttgc
     6301 tgcacggctt cggatcgagc ggctccgaca tgatctcgct ggctccggcc tgggccgacg
     6361 cgatgcccga cgctctcttc ctcgcgccgc acgcgccgca gcgctgcggg atgatgggcg
     6421 cgggctacca atggtgggcc ttgtcgggtt ttgcgccgtc tgccctagcc gcaggtgcgg
     6481 ccgctgcagc gcctgcaatc gacgccttca tcgaccgcaa gctggcgcag tatggtctga
     6541 ccgaggccga tcttgcgctg gtcggcttta gccaggggac gatgatggcg ctccacgtcg
     6601 ggctgcgccg gccgcgcgca gtcgcagcca tcgtggggta ttcggggatg ctcgccggca
     6661 cgctcgggct ggggcatggc gctctcccca aacccccggt ccttctggtg cacggaaccg
     6721 ccgatccggt ggtgccgatt gccgcgctcc acatgtccga gagcgaactc aaacgtctcg
     6781 ggatcgatgt gacgacgcac atctcttatg gtgtcgcgca tagcgtcgat cccgtgggcc
     6841 tgcgccttgg gcgagatttt gtaaccgaag ccttcgttca ctcgtcctga ccgcgcccgg
     6901 tcctctggac gaaaagcttt acgcgacgcc gcccattggc tcccgccttg tctggcgcgc
     6961 gccctccgcc ctgcgccgcg tgcaacccaa tcgcgcgctc tgcgtttgcg atctggttca
     7021 aacagggagt gacatcatga aaaagctttt gatcggtctt gccgccacct ccgctctcgc
     7081 gcttggcgct tgccagagcc ctgctgccga caaggtggaa gaccaggccg aagccaaggc
     7141 tgaggccatc gacgaccagg ccgacgcaat gcccgagggt cccgcgaagg aggccatgga
     7201 gcagaaggcg gatatcgtcg aagagaaggg cgaggaaaag gcgaacgcca tggacgataa
     7261 tggcgagatc gctccgtccg aaagcggcgt tggggacacg cagaagaaat aagcctgcct
     7321 cccctaaagg gcaggatcaa gggcgccggt ctccggcgcc cttttcttgt tccgcgagat
     7381 ccatctgcag cacttgccgt cgcttgcgtg accgcagcgt gcgccggaac aacggcacga
     7441 gcggaacgtt cgacctgcat gaccaccatg ggagcaggcg ggatgaacag actgaggcac
     7501 ctcgagacct tcgcccgcgc cggatggtgc gcgcggggcg ccgtctattg tcttctcgcg
     7561 acgtttgccc tgaccggcgc gggtgcttcg gacgccagtc cgcaaggggt gttccgctcg
     7621 gttcgcgaca tgccggcggg cacgctgctg ctcctgctgc tcaccgcggg gcttgcgctc
     7681 tacggcgcct accggctcta cggcgccgcg ctcgacagcg aaggcaaagg tagcgacgcc
     7741 aagggcatcg cgacccgcat cggctatgcc gcaagcgggc ttgcgcattt tgtgctcgcc
     7801 tgggcggccg cccgcctcgc c
//
LOCUS       DATMSU010000518         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_150408,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000518 DATMSU010000000
VERSION     DATMSU010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150408"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1251)
                     /locus_tag="VN034_13845"
     CDS             complement(145..1251)
                     /locus_tag="VN034_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HWV61740.1"
                     /translation="MSAAGWAIDIDRDDITQAKLVDDAATPLVPGQVRVHLDSYAMTA
                     NNITYAVFGKPAGLFGNDQGYWDFFAERDAPGRLPVWGFATVTESAAEDVSVGDRFYG
                     YYPMASDAILNVGKAGPGGFTDVTPRRMTLPPIYNHYQRVEALEGYRAADHDYWPVFR
                     PLFLTGWLIADQFEDEGDYGASQILIASASSKTAIGLGFSLARRAGHRPETVGLTSKA
                     NVADLDAQRIYDRVIAYDDILTLNPGTPSALVDMAGNGAVTRVVHSHFGNNLKASIIV
                     GKSHWDADAGGATLPGPERQGFFAPGRSEKRIADWGGAAFGQKIADAWLAFMDVAPRL
                     TSVDKRNGGDAALAAYREMLSGQTDPRKGIVVVP"
     gene            1309..1692
                     /locus_tag="VN034_13850"
     CDS             1309..1692
                     /locus_tag="VN034_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWV61741.1"
                     /translation="MAPPLTIAVKRIHEPAGPEEGTRFLVDRLWPRGVSKEKAALAAW
                     LKPLSPSDALRKRFHGETATSDEAWDAFRRDYFAELDTGGEEVKAALFTLDAAARAGP
                     VTLLYAAKDEERNNAVALREWLERR"
     gene            complement(1689..2381)
                     /locus_tag="VN034_13855"
     CDS             complement(1689..2381)
                     /locus_tag="VN034_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HWV61742.1"
                     /translation="MLHSETHRVDRIGWLRAAVSGANDGLVSTASLVVGVAAAATGHG
                     GILIAGVAGLAAGAMSMAAGEYVSVSSQGDAEKADVELEKRHLAADPEFELEELTLIY
                     QERGLGRALAEEVAAQLTAHNALDTHLRDELGMTDAMAARPVQAAAASAASFAIGAAL
                     PLATVLVDRGDTLPFTVSGASLVFLAILGALGAWAGKAPMLRPVVRVTFWGAMAMATT
                     IAIGSLLGTTVG"
     gene            2523..3560
                     /locus_tag="VN034_13860"
     CDS             2523..3560
                     /locus_tag="VN034_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HWV61743.1"
                     /translation="MIKYIGSKRALLGYVTGAVAGVLPQGGRVCDLFSGTARVGHALK
                     KQGFQVWSNDLNAYAHALATTYVQADRERWVERAEVVLAELRTVRPEPGWFTQAFCQD
                     ARYFHPDNGARIDAMRDRIEAMGLEPELKAIALVSLMEAADRVDSTAGVQMAYMKQWA
                     PRALKTLELRTPDLVAAVDGPCRATQGDAVEIAEQVEADLVYLDPPYNQHSYLGNYHC
                     WESLVLWDRPETYGVANKRVDVRTRKSAFNSRPGIGPALRTVIERVKAPNLIVSFNDE
                     GYLSRADLVEMLSARGHVQVLEMAHPRYVGARIGIHNPKGEKVGQVGRLKNVEHLFVV
                     TERPVSLPVAA"
BASE COUNT          597 a         1161 c         1227 g          581 t
ORIGIN      
        1 cttgcgcgcg ggcgcggcct tgggcgtttc ggcgagcgcc ggtcccgcgg gcagtagcag
       61 cgcgagcgag gcggcgaagg caaagatatg acgcatggtt tatcccttcc ggcggctagg
      121 aaaggaattg ctcccacgcg accctcatgg aacgacgacg atccccttcc tggggtcggt
      181 ctgtcccgag agcatctccc gataggcggc gagcgccgcg tcgccgccgt tgcgtttatc
      241 gaccgaagtc agccgcggcg caacgtccat gaaggcgagc caggcgtcgg cgatcttctg
      301 cccgaacgcc gcgccgcccc aatcggcgat gcgtttctcg ctgcgtcccg gcgcgaaaaa
      361 gccctgtcgc tcggggccgg gaagcgtggc cccgccggca tcggcgtccc agtgcgactt
      421 gccgacgatg atagaggcct tgagattgtt gccgaaatgg ctgtgcacga cgcgcgtgac
      481 cgcgccattg cccgccatgt cgacgagcgc cgacggcgta ccgggattga gcgtcaagat
      541 gtcgtcatag gcgatgacgc ggtcgtagat gcgttgcgca tcgaggtcgg cgacattggc
      601 cttgctggtc aggccgaccg tttcggggcg atggccggcg cgccgcgcga gcgaaaagcc
      661 gaggccgatc gcggtcttgc tcgacgcgct cgcgatcagg atctggctcg cgccatagtc
      721 gccctcatcc tcgaactggt cggcgatcag ccagccggtc aggaacagcg ggcggaacac
      781 cggccaataa tcatggtcgg cggcgcgata gccttcgagt gcctcgaccc gctgataatg
      841 gttgtagatc ggcggcagcg tcatgcggcg cggggtgacg tcggtgaaac cacccggtcc
      901 cgccttgccg acattgagta tcgcatcgct cgccatcggg taatagccgt agaagcggtc
      961 gccgaccgaa acgtcctcgg cggcgctttc ggtcaccgtc gcaaaacccc agacgggcag
     1021 gcggccgggc gcgtcgcgct cggcgaagaa atcccaatag ccttgatcgt ttccgaacag
     1081 ccccgcgggc ttgccgaaca ccgcgtaggt gatgttgttc gcggtcatcg catagctgtc
     1141 gaggtgaacg cgtacctgtc cggggaccag cggcgtggca gcgtcgtcga ccaatttcgc
     1201 ttgcgtgata tcgtcgcgat cgatgtcgat cgcccagcct gctgcgctca tgtccggacc
     1261 cctccgattg gcttgcccgc gggcgcggga tggccgatag tcatacagat ggcgccgcct
     1321 ctgacaatcg cggtcaaacg catccacgaa ccggctggtc ccgaggaggg cacgcgtttc
     1381 ctcgtcgacc ggctgtggcc gcgcggggtc tcgaaggaaa aggcggcgct cgcggcgtgg
     1441 ctcaagccgc tgtcgcccag cgatgcctta cgcaagcgct tccacggcga gaccgccaca
     1501 tcggatgaag cctgggacgc cttccgccgc gactatttcg ccgaactcga tacgggcggc
     1561 gaggaggtga aggcggcgct tttcaccctc gacgcggcgg cgcgggcggg gcccgtgacc
     1621 ttgctctacg cggcgaagga cgaggagcgg aacaatgccg tcgcgctccg cgaatggctg
     1681 gagcggcgct agcccaccgt cgttccgagc agcgatccga tcgcgatcgt cgtcgccatc
     1741 gccatcgcgc cccagaaggt cacgcgaacg accgggcgta gcatcggcgc cttgcccgcc
     1801 catgctccga gcgcgccgag gatcgcgagg aagacgagcg acgcgcccga cacggtgaag
     1861 ggcaaggtat cgccccgatc gacgagcacc gtcgcgagcg gcaatgccgc gccgatcgcg
     1921 aaactcgccg cggaggccgc tgccgcctgc accggccgcg ccgccatcgc gtcggtcatg
     1981 ccgagttcgt cgcgcagatg cgtgtcgagc gcattgtgcg cggtaagctg cgccgcgacc
     2041 tcttcggcga gcgcgcgacc cagcccgcgc tcctgataga ttaaagtcag ctcctcgagc
     2101 tcgaactcgg gatcggcggc aaggtggcgc ttctccagct cgacgtcggc cttttcggcg
     2161 tcgccctgcg agcttaccga gacatattcg cccgccgcca tcgacatcgc gcctgccgcc
     2221 aatccagcga cgcccgcgat caggatgccg ccatgaccgg tcgccgccgc cgcgacgccc
     2281 accaccagac tggccgtcga caccagcccg tcattggccc ccgacaccgc cgccctaagc
     2341 cagccgatcc gatcgacccg atgggtttca ctgtgcagca tacgccttct ccgtagtcgt
     2401 cgcaccgagc atggagcggg cgttggcttg aagctaggcg cggcgtttat atcccgacga
     2461 tccgggcaac ttcgcgcgcc ccgcgatagc cgctatgctg ccgcgatgag tcgggccgcg
     2521 tcatgatcaa atatatcggc tccaagcgcg ccttgttggg gtatgtaacg ggtgcggtgg
     2581 cgggtgtgct gccgcagggc ggccgggtgt gcgatctgtt ttcgggcacg gcgcgggtcg
     2641 gacacgccct gaagaagcag gggtttcagg tgtggtcgaa cgacttgaac gcctatgccc
     2701 acgccctggc gacgacctat gtccaagcgg atcgagagcg gtgggtcgag cgggccgagg
     2761 tcgtgctggc cgagttgcgg acggtgaggc ctgagccggg ctggttcacc caagccttct
     2821 gtcaggacgc gcggtatttc cacccggaca acggtgctcg catcgacgcc atgcgcgacc
     2881 ggatcgaggc catgggactg gaaccggaac tcaaggccat cgccctggtc agcctgatgg
     2941 aggcggcgga tcgggtggat tccaccgccg gggttcagat ggcctatatg aagcagtggg
     3001 cgccgagggc gctgaagacg ctggagctgc ggacgccgga tctggttgcg gcggtggacg
     3061 ggccgtgccg cgcgacccag ggcgatgcgg tcgagatcgc tgaacaggtc gaggcggacc
     3121 tggtttatct ggacccgccc tacaaccagc attcctatct ggggaactac cattgctggg
     3181 agagcctggt gctgtgggac cggcccgaga cctatggggt ggccaacaag cgggtggacg
     3241 tgaggacgcg taagagcgcc ttcaacagcc ggcccggcat cggcccggcg ctgcggaccg
     3301 tgatcgagcg ggtcaaggcg ccgaacctga tcgtcagctt caacgacgag gggtatctga
     3361 gccgcgccga cctggtcgag atgctgtcgg cgcgaggcca cgtgcaggtg ctggagatgg
     3421 cgcatccgcg ctatgtcggc gcccggatcg ggatccataa tcccaagggc gagaaggtcg
     3481 ggcaggtggg gcggctcaag aatgtcgagc acctgttcgt ggtcaccgag cgaccggtct
     3541 ccctgcccgt ggccgcatag acacca
//
LOCUS       DATMSU010000519         6320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_169323,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000519 DATMSU010000000
VERSION     DATMSU010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169323"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="VN034_13865"
     CDS             <1..1535
                     /locus_tag="VN034_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007015007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWV61744.1"
                     /translation="KAGLDHATRTSLAVVESAYDVALAGFALPYGDVAVGGWRNTPYV
                     VIQNVGAYLDIPKFLDSDHPVKSSADAEAYLSRLNAFSGVLDGETGRLKAAGGQGLIA
                     PAFLIDKAVTAMEATIADAKAGGSMVESLVRRTGEAKIAGDWSARSAKIVQGPVAAAL
                     ERQLAELKAQRPKATMDAGMWARPGGDEWYAWGLRASTTTRMTPDEIHAMGREELAEL
                     HGRMDPILKKLGYTQGSVGDRMNALAKDPKYKFPDNDQGRAEIVAYIQTWLGKIRAEL
                     PRAFRTLVKGNVEVKRLPLAEEPGAPAAYGGAGSIDGSIPGRFWINLRTTELHSKYSL
                     PDLTMHEAIPGHAWQGEYAHAMPLIRTMLAFNAYSEGWALYAEQLADELGLYDDFEVG
                     RLGYLQSLAFRACRLVVDTGLHAKRWTREQGVEFFVKENGSNPLEVASEVDRYCSWAG
                     QACGYKVGHSEIVRQRGLAQKALGAKYDLRDFNDVVVKGGNVPLDVLAKNVDEYVREA
                     KA"
     gene            1547..2470
                     /locus_tag="VN034_13870"
     CDS             1547..2470
                     /locus_tag="VN034_13870"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HWV61745.1"
                     /translation="MRSLLSIALLAATPAYAHESAEPHGAARVTIVTSDVDRFYALYD
                     DPALAAQPAVVASRYLASPSPGLAEFMVMRRITPEKLAAALSKKPQVFADARGCAANL
                     PDVRARLVAATDRLAQIYPAAKFPPITIAIGRATTAGTANAKGLYIGLEALCAAKFIE
                     ADDEDRFVHVIAHEYVHAQQPLAQVEDREESVLRAALVEGAAEFVAERMSGSVGYPLL
                     HRWAKGREMELETAFLAEKDQKAIGSRWLYNQKGTDGWPGDLGYWVGYRVAKAYYDRA
                     PDKAAAIKAIIEMKDPAAFLAASGWTPGMAL"
     gene            complement(2467..2916)
                     /locus_tag="VN034_13875"
     CDS             complement(2467..2916)
                     /locus_tag="VN034_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61746.1"
                     /translation="MTTEYKPRLFARDEDIRALGEGLLACALPREAWTHEAHLAACLW
                     LLAERPDIDVDAEIAGLIRRFNESVGGVNDDHGGYHDSITRAYVAGVRLFLRETAETE
                     LAARVNALLLSEIGRRDWPLRFYSRDLLFSVAARRGFVEPDLAPLPG"
     gene            complement(2966..4567)
                     /locus_tag="VN034_13880"
     CDS             complement(2966..4567)
                     /locus_tag="VN034_13880"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HWV61747.1"
                     /translation="MSKVVIGSDTVETQADVAENWGTSALIEDAVRHDEGRLAKDGPL
                     VVETGKHTGRSAKDKFIVQDDETADTIWWGKTNVPMTPDHFAALKADFLAHLAERPRL
                     YRQQLFGGSQPEHRVAVQVVTEFAWHSQFIRTLLCRPTAAELADFAAEYTIVDLPSFR
                     ADPAKHGTRSETVIAVNFTEKLILIGGTQYAGEMKKSVFGILNYLLPVKGVMPMHCSA
                     NIGANGDTAVFFGLSGTGKTTLSADASRTLIGDDEHGWSDTAVFNFEGGCYAKMIRLS
                     PEAEPEIFATTKRFGTILENVVMDPETRTLDFDDASLAENSRGSYPIDFIPNTSEHNM
                     GPVPKTVIMLTADAYGVLPPIAKLTPDQAMYHFLSGYTARVAGTEIGVTEPEATFSTC
                     FGAPFMPRHPSVYGNLLKERIAKGGVQCWLVNTGWTGGMATMDGIKRMPIKATRALLN
                     AALDGSLNDVEFRKDPNFGFLVPVAVPGVDSSLLDPREAWADKAAYDRTAQTLVGQFI
                     DNFAQFAEHVDEGVRQAAPRAAVAA"
     gene            4848..5558
                     /locus_tag="VN034_13885"
     CDS             4848..5558
                     /locus_tag="VN034_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV61748.1"
                     /translation="MTATIALVDDDRNILQSLSIALQAEGFVTRVYSDGEAALKPLID
                     NPPDLAVFDIKMPRMDGLELLRRLREKSQLPVIFLTSKDDEIDEALGLAMGADDYIAK
                     PFSQRLVIARIRAILRRVELNRSAPSEDDEPVAEPITRGRLSMDPARHKVAWDGKDVT
                     LTVTEFLILETLASRPGVVRSRNQLMDAAYQDDVYIDDRTIDSHIKRLRRKFREVDPE
                     FDAIATLYGAGYRFADES"
     gene            5548..>6320
                     /locus_tag="VN034_13890"
     CDS             5548..>6320
                     /locus_tag="VN034_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HWV61749.1"
                     /translation="MKADVAKHERLKPDASIAEQEESPLVWSARWSLTTRILAVNILA
                     VALLAGGFYYLDTYRSRLVDTRIEVMKDQLAMLDSALEAARPDQRATLIREFTKATES
                     RLRFYGADGRRVSDSFASGPPTYTLRDPELQPWKRDAARAMDRGIDWIVGAPSYPDFE
                     EPRDDRARSWPEVVEAQQSGLAASRFRYAPERTPLLSAARVVDLETPQTVLMTLNARD
                     ITRTVRAERFRLAVVILVVLMASVLLSLFLARTIVRPLRR"
BASE COUNT         1081 a         2107 c         2102 g         1030 t
ORIGIN      
        1 acaaggcggg gctcgaccat gccacgcgca ccagcctcgc cgtcgtcgaa agcgcctatg
       61 acgtcgcgct cgcgggcttc gcgctgccct atggcgacgt cgcggtcggc ggctggcgca
      121 acacgcccta tgtcgtgatc cagaatgtcg gcgcctatct cgacatcccc aaattcctcg
      181 acagcgacca tccggtcaag agctcggccg atgccgaggc ttatctttcg cgcctcaacg
      241 ccttttccgg cgtgctcgac ggcgagaccg gccggctgaa ggcggcgggc ggccaggggc
      301 tgatcgcccc cgccttcctg atcgacaagg cggtgacggc gatggaagcg acgatcgccg
      361 acgcgaaggc gggtggctcg atggtcgaaa gcctcgtccg ccgcaccggc gaagcgaaaa
      421 tcgccggcga ctggtcggcg cgctcggcga agatcgtgca gggccccgtc gccgcggcgc
      481 tcgaacgcca gctcgccgag ctgaaggcgc agcggccgaa ggcgacgatg gacgcgggca
      541 tgtgggcgcg tcccggcggc gacgaatggt atgcgtgggg gctccgcgcc tcgacgacca
      601 cgcgcatgac ccccgacgag attcacgcga tgggccgcga ggagctcgcc gaactccacg
      661 gccggatgga tccgatcctc aagaagcttg gctatacgca aggctcggtc ggcgaccgga
      721 tgaacgcgct cgccaaggat cccaaatata aattccccga caatgatcag ggccgcgccg
      781 agatcgtcgc ctatatccag acctggctcg gcaagatccg cgccgagttg ccgcgcgcct
      841 tccgcaccct cgtgaagggc aatgtcgagg tgaagcggct cccgctcgcc gaggagcccg
      901 gcgcgcccgc ggcctatggc ggcgcggggt cgatcgacgg cagcattccc gggcgcttct
      961 ggatcaacct ccgcacgacc gagctgcaca gcaaatattc gctgcccgat ctgacgatgc
     1021 acgaggcgat cccgggccat gcgtggcagg gcgaatatgc gcatgcgatg ccgctcattc
     1081 gcaccatgct ggcgttcaac gcctattcgg aaggctgggc gctatacgcc gaacagctcg
     1141 ccgacgagct tggcctctat gacgatttcg aggtcgggcg ccttggctat ctccagtcgc
     1201 tcgccttccg cgcctgccgc ctcgtcgtcg acaccggcct ccacgccaaa cgctggacgc
     1261 gcgagcaggg cgtcgaattc ttcgtcaagg agaatggctc gaacccgctc gaggtcgcga
     1321 gcgaggtcga ccgttattgc agctgggccg gacaggcgtg cggatacaag gtcggacaca
     1381 gcgagatcgt gcggcagcgc ggcctcgcgc aaaaggcgct gggcgccaaa tatgacctgc
     1441 gcgatttcaa cgacgtcgtc gtcaagggcg gcaatgttcc gctcgacgtg ctcgcgaaga
     1501 atgtcgacga atatgtgcgg gaggcgaagg cgtgaggacc cgcaggatgc gaagcctgct
     1561 gagcatcgcg ctgctcgctg cgaccccggc ttacgcgcat gagtcggccg agccgcacgg
     1621 cgcggcgcgc gtcaccatcg tgacgagcga cgtcgatcgc ttttacgcgc tctatgacga
     1681 ccccgcactc gcggcgcagc ccgcggtggt ggcgtcgcgc tatctcgcct ccccctcgcc
     1741 cgggctcgcg gaattcatgg tgatgcgccg gatcacgccc gaaaagctcg ccgccgcgct
     1801 gagcaagaaa ccgcaggtct ttgccgacgc gcgcggctgc gcggcgaatc ttcccgatgt
     1861 ccgcgcgcgc ctcgtcgccg ccaccgaccg cctcgcgcaa atctatcccg ccgcgaaatt
     1921 tccgccgatc acgatcgcga tcggccgcgc gacgaccgcg ggaaccgcca atgcgaaagg
     1981 gctctacatc ggcctcgaag ccttgtgcgc cgccaaattc atcgaagccg acgacgagga
     2041 ccgcttcgtg catgtcatcg cgcacgaata tgtccacgcg cagcagccgc tcgcgcaggt
     2101 cgaggatcgc gaggaatcgg tgctgcgcgc ggcgctcgtc gagggcgccg ccgaattcgt
     2161 cgccgagcgg atgtcgggct cggtcggcta tccactgctc catcgatggg ccaaggggcg
     2221 cgagatggag ctggagaccg ccttcctcgc cgaaaaggac caaaaggcga tcgggtcgcg
     2281 ctggctttac aaccagaagg gcaccgacgg ctggccgggc gacctcggct attgggtcgg
     2341 ctaccgcgtc gccaaagcct attacgaccg cgcgcccgac aaggcggcgg cgatcaaggc
     2401 gatcatcgaa atgaaggatc cggcggcgtt tctcgcagcg agcgggtgga cgccggggat
     2461 ggcgctttag ccgggcagcg gcgccaagtc gggttcgacg aagccgcgcc gcgcggcgac
     2521 cgaaaagagc aagtcgcggc tgtagaagcg cagcggccag tcgcggcggc cgatctcgga
     2581 cagcagcagc gcgttgacgc gcgccgcgag ctccgtttcg gccgtctcgc gcaggaacag
     2641 cctgaccccc gcgacatagg cgcgcgtgat gctgtcgtga tagccgccat gatcgtcgtt
     2701 cacgccgccg acgctttcgt tgaagcggcg gatcaggcct gcgatctcgg catcgacgtc
     2761 gatgtcgggg cgttcggcga gcagccagag gcacgcggcg agatgcgcct cgtgcgtcca
     2821 tgcttcgcgc ggcaaggcgc acgcgagcaa gccttcgccc agcgcgcgga tgtcttcgtc
     2881 gcgcgcgaac aggcgcggtt tatattcggt ggtcatgggt ccggtccttc cgggtgagct
     2941 gccacccgga agcgatccgg gtcaatcagg ctgcgacggc cgcccgcggt gcggcttggc
     3001 ggacgccttc gtcgacatgc tccgcaaatt gcgcgaaatt gtcgatgaat tggccaacga
     3061 gcgtttgcgc cgtgcggtcg tacgccgcct tgtcggccca ggcttcgcgc gggtcgagca
     3121 gcgacgaatc gacgcccggc accgcgacgg ggaccaggaa gccgaagttc ggatccttgc
     3181 ggaattccac gtcgttgaga ctgccgtcga gcgcggcgtt gagcagcgcg cgggtcgcct
     3241 tgatcggcat gcgcttgatg ccgtccatcg tcgccatgcc gccggtccag ccggtgttga
     3301 cgagccagca ctgcacgccg cccttggcga tgcgttcctt gaggaggttg ccatagaccg
     3361 acggatggcg cggcatgaaa ggcgcgccga agcaggtcga gaaggtcgcc tcgggctcgg
     3421 tcacgccgat ctcggtcccc gcgacgcgcg cggtatagcc cgagaggaag tgatacatcg
     3481 cctgatcggg ggtcagcttc gcgatcgggg gcagcacgcc ataggcatcg gcggtgagca
     3541 tgatcaccgt cttgggcacc gggcccatat tatgctccga cgtgttcgga atgaagtcga
     3601 tcgggtagct gccgcggctg ttctcggcga ggctcgcatc gtcgaagtcg agggtgcgcg
     3661 tttcggggtc catcacgaca ttttcgagga tcgtgccgaa gcgtttggtg gtcgcgaaaa
     3721 tctcgggctc cgcctcgggc gagaggcgga tcatcttggc atagcagccg ccctcgaagt
     3781 tgaagaccgc ggtgtccgac cagccatgct cgtcgtcgcc gatcagcgtg cgcgacgcat
     3841 cggccgacag ggtcgtcttg ccggtgcccg agaggccgaa gaagaccgcg gtgtcgccat
     3901 tcgcgccgat gttcgccgag cagtgcatcg gcatcacgcc ctttacgggg agcagatagt
     3961 tcaagatacc gaacaccgac ttcttcatct cgccggcata ttgcgtgccg ccgatcagga
     4021 tcagcttttc ggtgaagttg accgcaatca ccgtctcgct gcgcgtgccg tgcttcgcgg
     4081 ggtcggcgcg gaagctcggc agatcgacga tcgtatattc ggccgcgaaa tccgcgagtt
     4141 cggccgcggt cgggcggcag agcagggtgc ggatgaactg gctgtgccag gcaaattcgg
     4201 tgacgacctg caccgcgacg cgatgctcgg gctgcgagcc gccgaacagc tgctggcgat
     4261 agaggcgcgg acgctcggcg aggtgcgcga ggaaatcggc cttcagcgcc gcgaaatgat
     4321 cgggggtcat cgggacgttg gtcttgcccc accagatcgt gtccgccgtt tcgtcgtcct
     4381 ggacgatgaa cttgtccttg gcgctgcgcc cggtgtgctt gcccgtctcg acgacgagcg
     4441 ggccgtcctt ggcaagccgg ccttcatcgt ggcgcaccgc atcctcgatc agcgccgacg
     4501 tgccccagtt ttcggccacg tccgcctgcg tttcgacggt atcgctgccg atcacaacct
     4561 tgctcatttg cttcgccttt ccttgtccgg ccccgccgcc tgcataccta tgcaatgctt
     4621 agcgcgctaa tgggctggcc cgcatctgca tgggtgccga cgtgtccgcc gattccgaaa
     4681 tcccggccgc gttagcgagg gcgggagcgg aggtcaaaag attaacgtgg cgcagtgcag
     4741 catgtgcgac gagcgtttgg cgagggaacc ggtgccatgc gcgcgcggtt gtcgctgaac
     4801 gccggatagg ctaatcgctc ccctggagcc cgcccaatgg aaagagcatg acggcaacca
     4861 tcgcgctggt cgacgacgac cgcaatatcc tgcaatcgct gtcgatcgcg ctgcaagccg
     4921 aaggattcgt aacgcgcgtc tattcggatg gcgaagcggc gctgaagccg ctgatcgaca
     4981 atccgcccga cctcgccgtc ttcgacatca agatgccgcg gatggacggg ctcgaactgc
     5041 tgcgccggct gcgcgagaag agccagcttc cggtcatctt cctgaccagc aaggacgacg
     5101 agatcgacga ggcgctgggc ctcgcgatgg gcgccgacga ctatatcgcc aaaccctttt
     5161 cgcagcgcct ggtgatcgcg cgcatccgcg ccatcctgcg ccgcgtcgag ctcaaccgga
     5221 gcgcgccgag cgaggacgac gagccggtcg ccgagccgat cacgcgcggg cggctgagca
     5281 tggaccccgc gcggcacaag gtcgcatggg acggcaagga tgtgacgctg accgtcaccg
     5341 aattcctgat cctcgaaacg ctcgccagcc gccccggcgt cgtgcgcagc cgcaaccagc
     5401 tgatggacgc cgcctatcag gacgacgtct atatcgacga ccgcacgatc gacagccaca
     5461 tcaaacgcct gcgccgcaag ttccgcgagg tcgatcccga attcgacgcg atcgcgaccc
     5521 tctatggcgc gggttaccgc tttgccgatg aaagctgatg tcgcaaaaca cgagcggctg
     5581 aagcccgacg cgagtatcgc cgagcaggag gaatcgccgc tcgtctggtc ggcgcgctgg
     5641 tcgctgacca cgcgcatcct ggcggtcaac atcctcgccg tcgcgctgct cgccggcgga
     5701 ttctattatc tcgacaccta tcgcagccgg ctggtcgaca cgcgcatcga ggtgatgaag
     5761 gaccagctcg cgatgctcga cagcgcgctc gaagccgcac gccccgacca gcgcgccacc
     5821 ctgatccgcg aattcaccaa ggcgaccgaa tcgcggctgc gcttctacgg cgccgatgga
     5881 agacgcgtct ccgacagctt cgcgagcgga ccgccgacct atacgctgcg cgatcccgag
     5941 ctccagccgt ggaaacgcga tgccgcgcgc gcgatggatc gcggcatcga ctggatcgtc
     6001 ggcgcgccga gctatcccga tttcgaggaa ccgcgcgacg accgggcgcg gtcgtggccc
     6061 gaggtcgtcg aggcacagca aagcggcctc gcggcgagtc gcttccgcta tgcgcccgag
     6121 cgcaccccgc tgctcagcgc cgcgcgcgtc gtcgatctcg aaacgccgca gacggtgctg
     6181 atgacgctca acgcgcgcga catcacgcgg acggtgcgcg ccgaacgctt tcgcctcgcg
     6241 gtggtcatcc tcgtcgtgct gatggcgtcg gtgctgctct cgctcttcct cgcgcgcacg
     6301 atcgtccgcc ccttgcgccg
//
LOCUS       DATMSU010000520         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_37064, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000520 DATMSU010000000
VERSION     DATMSU010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37064"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1889
                     /locus_tag="VN034_13895"
     CDS             105..1889
                     /locus_tag="VN034_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61750.1"
                     /translation="MTPPPPPVEWKETVSDDLMTQIIEGRRRPGYRPDLPDPLSQDNE
                     GALRPPPPQAFPGMEDQLPVPDRWRLIETLGVVKERWFDPYHQNTLKGDRPIDRAKVP
                     WLPIKGDDWFFALNAVSDSVLEPRTFPIPVGIQYPDRPGSNDVFGRPESLVASQTFIV
                     GAALIKGNTTYKPPDVEYRLTLAYNLNYVEVPERRILFVEPSKGRTRFDSFLGVQEAF
                     IDYHLQNTSDRYDFDSVRVGIQPFQADFRGFLFQDSQLSYRLFGNRDNNRIQYNLVAV
                     WRLEKDTNSGLNDLTQSPRRDWILHANLYRQDFPFVGLTSEISATWNINREANRVQID
                     DNGFPVRPALIGNLQGRDYDAIYLGYGVDGRIGRINVTAMGYAALGSDRDNVFTGRKA
                     DIRSYFGAAELSYDHDWMRFRLSGLYATGDKDPFDDKQTGFDAIFENPIFAGADTSYW
                     IRQAVPFIGGGRAIGLTGRNGILNSLRSSKEEGQSNFVNPGTVLLGAGADFDLTPQFR
                     LSTNANHLWFDDTKVLQVLRSEGSIPKAIGWDLSAAAIWRPKATQNIVGRLSGAVLLP
                     GAGFKDLFENQRRNKAYVSILANVILSF"
     gene            1926..>4384
                     /locus_tag="VN034_13900"
     CDS             1926..>4384
                     /locus_tag="VN034_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61751.1"
                     /translation="MVSALFAGAADLGAAEKEKPVKVEYRFTPPAPREQGDAEVAAKS
                     NGCYSCHTRTDQPSMHTTPAVKLGCTDCHGGDSLSPAAFGKPELGYKHPDNIAAMKVA
                     HPQPTYPKSWGDTSANPVRSYTLLNREAPEFIRFVNPSDYRVAREACGACHLEVIEAT
                     ERSLMSTGAMLWGGAAYNNGIVPYKNYIFGEAYTRTGEAACILSPSSRLTAEEYKEAC
                     KPSFPSDKILTDAEEKRGALAKMYPLPRWNVIPPGDVFRVFERGGRNINSQFAEVGLP
                     NPTGSIQRLEEPGRPDLKQSNRGPGTGLRVSIPVLNIHKTRLNDPLSWFMGTNDQPGD
                     YRHSGCSGCHVVYANDREPRHSLTYAKFGRDGETATIDPTIAEKKRKPAGYGDGHGGD
                     AHSAKDGHQAGGGHGSLIDPADPDVPARPGSPARGESGHPISHAFTRAIPTSQCMSCH
                     MHQPNIFLNSYLGYTMWDYESDAPQMWPGPDNTAPRPPGMSDEEYQKKYKQQRYPTAA
                     EVHAVLERNPEAASARGLWSDVEFLRDVYDVNDTNKDTQFADYHGHGWNFRGIFKRDR
                     SGNLLTADGNMATYGTDTANIVDPKDPEKWRKSCSHYTDPKERDLCLASADPEKPGVE
                     GKFVPPGLNPGKTVHMMDIHAEKGMQCADCHFAQDSHGNGFIYGEVANAIEIGCKDCH
                     GTADAYPTLLTSGPAAPPKGNNLALLRNPDGKRRFEWFYDASGKRKLIQRSIVDPKLE
                     WEVSLVKDSVDPANPHFNAKAARAKLMSKSGAEDGSFSFGPGVAKADRAHGDDSMACF
                     TCHLSWTTSCGGCHLPIEANW"
BASE COUNT          859 a         1500 c         1334 g          691 t
ORIGIN      
        1 ctcccggcac cgatgcgcct gcgcagccgc ccgaagccgg cccggccgcg gcggccgatg
       61 ccgctcctgc tgatactacc acgagggaac aacccggcga cgaactgacc ccgccgccgc
      121 cgcccgtcga atggaaagag acggtcagcg acgatctgat gacgcagatc atcgaagggc
      181 ggcgccggcc cggctatcgg cccgatcttc ccgatccatt gtcgcaggac aatgaaggcg
      241 cgctgcgtcc gccgccgccg caggcctttc cggggatgga ggaccagctt cccgttcccg
      301 accgctggcg cctcatcgag acgctgggcg tcgtcaagga acgctggttc gatccctatc
      361 accagaatac gctgaagggc gaccggccga tcgaccgcgc gaaagtgccg tggctgccga
      421 tcaagggcga cgactggttc ttcgcgctga acgcggtgtc ggacagcgtg ctcgaaccgc
      481 gcaccttccc gatccccgtc ggcatccaat atcccgaccg gccgggcagc aacgacgtct
      541 tcggccgccc cgaaagcctc gtcgcgtcgc agaccttcat cgtcggcgcg gcgctgatca
      601 agggcaatac gacctacaag ccgcccgacg tcgaatatcg cctgacgctg gcgtacaacc
      661 tgaattatgt cgaggttccc gaacggcgga tcctgttcgt cgagccgtcg aagggacgga
      721 cgcgcttcga cagctttctg ggcgtccagg aagccttcat agattaccac ctgcagaaca
      781 cgtcggatcg ctacgacttc gattcggtgc gcgtcggcat ccagccgttc caagcggatt
      841 tccgcggctt cctgtttcag gacagccagc tcagctatcg cctcttcggc aaccgcgaca
      901 acaaccggat ccagtataat ctggtcgccg tctggcgcct cgaaaaggac acgaacagcg
      961 ggctcaacga cctgacccag tcgccgcgca gggactggat cctgcacgcc aatctctacc
     1021 gccaagactt cccgttcgtc gggttgacca gcgaaatttc ggcaacgtgg aacatcaacc
     1081 gcgaagcgaa ccgcgtccag atcgacgaca atggctttcc cgtgcggccg gcgctgatcg
     1141 gcaatttgca gggccgcgat tatgacgcga tctacctcgg ctatggcgtc gacgggcgga
     1201 tcggccggat caacgtcacc gcgatgggct atgccgcgct cggcagcgac cgcgacaatg
     1261 tcttcacggg gcgcaaggcg gacatccgat cctatttcgg ggcggccgag ctcagctatg
     1321 atcatgactg gatgcgcttc cgcctgtcgg gcctctatgc gacgggcgac aaggatccct
     1381 tcgacgacaa gcagacgggc ttcgacgcga tcttcgaaaa tccgatcttc gcgggcgccg
     1441 acaccagcta ctggattcgc caggcggtgc ccttcatcgg cggcggccgc gcgatcgggc
     1501 tgaccggccg caacggaata ctcaattcgc tgcgttcgtc gaaggaagag gggcagtcga
     1561 acttcgtcaa tcccggcacg gtgctgctcg gcgccggcgc cgatttcgac ctgaccccgc
     1621 aattccgcct ttcgaccaac gccaaccatc tctggttcga cgacaccaag gtgctgcagg
     1681 tgctgcgcag cgaagggtcg atcccgaagg cgatcggctg ggacctgtcg gcggcggcga
     1741 tctggcggcc caaggcgacg cagaatatcg tcggccgcct cagcggcgcg gtgctgctgc
     1801 ccggcgccgg gttcaaggat ctgttcgaga accagcggcg caacaaggcc tatgtgtcga
     1861 tcctcgccaa tgtgattttg agtttctagc ccatgatgcg cacgctcctc atcctgtttg
     1921 ccttcatggt ctcggcgctg ttcgcgggcg cggcggacct cggcgcggcc gaaaaggaaa
     1981 agccggtcaa ggtcgaatat cgcttcaccc cgcccgcgcc gcgcgagcag ggcgacgccg
     2041 aggtcgcggc gaaatcgaac ggctgctatt cgtgccacac gcgcaccgac cagccgtcga
     2101 tgcacacgac ccccgcggtg aagctcggct gcaccgactg tcacggtggc gattcactgt
     2161 cgcccgccgc cttcggcaag cccgagctcg gctacaagca tcccgacaat atcgccgcga
     2221 tgaaggtcgc gcacccgcag ccgacctatc cgaagagctg gggcgatacc tcggccaatc
     2281 ctgtgcgcag ctacaccttg ctcaaccgcg aggcacccga attcatccgc ttcgtcaatc
     2341 cgagcgatta ccgcgtcgcg cgcgaggcgt gcggggcgtg ccacctcgaa gtgatcgagg
     2401 cgaccgaacg ctcactcatg tcgaccggcg cgatgctgtg gggcggcgcg gcgtacaaca
     2461 acggcatcgt cccctataag aactatattt tcggcgaggc ctatacgcgc accggcgaag
     2521 cggcgtgcat cctgtcgccc tcgtcgcggt tgaccgccga ggaatataag gaagcgtgca
     2581 agccgagctt tccttccgac aagatattga ccgatgccga ggaaaagcgc ggcgcgctcg
     2641 cgaaaatgta tccgctgccg cgctggaacg tcattccgcc gggcgacgtc ttccgcgtct
     2701 tcgaacgcgg cgggcgcaac atcaattcgc aattcgccga ggtcggcctg ccgaacccga
     2761 ccggcagcat ccagcggctc gaggaaccgg ggcgccccga cctcaaacag tcgaaccgcg
     2821 gtccgggcac gggcctgcgc gtctcgatcc ccgtgctcaa catccacaag acgcgcctca
     2881 acgacccgct gtcctggttc atgggaacga atgaccagcc cggcgactat cgccattcgg
     2941 gctgttcggg ctgccacgtc gtctatgcca acgaccgcga gccgcgccac agcctgacct
     3001 atgcgaagtt cggccgcgac ggcgagaccg cgacgatcga tccgacgatc gccgaaaaga
     3061 aacggaagcc cgcgggctat ggcgacgggc atggcggcga cgcccacagc gccaaagacg
     3121 gccaccaggc tggcggcggc cacgggtctt tgatcgatcc cgccgatccg gacgtcccgg
     3181 cgcgccccgg atctccggcg cgcggcgaat cgggccatcc gatcagccac gccttcacgc
     3241 gcgcgatccc gacctcgcag tgcatgtcgt gccacatgca ccagccgaac atcttcctta
     3301 actcctacct cggctacacc atgtgggatt atgaatcgga cgcgccgcaa atgtggccgg
     3361 gccccgacaa caccgcgccg cgcccgccgg ggatgagcga cgaggaatat caaaagaaat
     3421 acaagcagca gcgctatccg accgccgccg aagtccacgc ggtgctcgaa cgcaatcccg
     3481 aggccgcgtc ggcgcgcggg ctctggtccg acgtcgagtt cctgcgcgac gtctatgacg
     3541 tcaacgacac gaacaaggac acgcagttcg ccgactatca cggtcatggc tggaatttcc
     3601 gcggcatctt caagcgcgac cgcagcggca atctgctgac cgccgacggc aatatggcaa
     3661 cttacggcac cgacaccgcc aacatcgtcg acccgaagga ccccgaaaag tggcgcaaga
     3721 gctgtagcca ttacaccgac cccaaggaac gcgacctctg cctggcgagc gccgatcccg
     3781 agaagcccgg tgtcgagggc aagttcgtgc cgccgggtct caaccccggc aagacggtcc
     3841 atatgatgga catccacgcc gaaaagggca tgcaatgcgc cgactgccac ttcgcgcagg
     3901 acagccacgg gaacggcttc atctatggcg aggtcgcgaa cgcgatcgag attgggtgca
     3961 aggattgcca tggcacggcc gacgcctatc cgacgctgct gacttcgggg cctgccgctc
     4021 cacccaaggg gaacaacctg gcgcttctgc gcaacccgga tggcaagcgg cgcttcgaat
     4081 ggttctacga cgcgagcggc aagcgcaaac tgatccagcg gtcgatcgtc gatccaaagc
     4141 tcgaatggga ggtgagcctc gtcaaggaca gcgtcgatcc cgccaacccg catttcaacg
     4201 ccaaagccgc gcgcgccaag ctgatgagca aatcgggcgc ggaggatggc agcttctcct
     4261 ttggccccgg cgttgcaaag gcagaccgcg cgcacggcga cgattccatg gcctgcttca
     4321 cctgccacct cagttggacg acgagctgcg gcggctgcca cctgccgatc gaggcgaact
     4381 ggaa
//
LOCUS       DATMSU010000521         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_75270, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000521 DATMSU010000000
VERSION     DATMSU010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75270"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..587
                     /locus_tag="VN034_13905"
     CDS             69..587
                     /locus_tag="VN034_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWV61752.1"
                     /translation="MTTITLARDEDVGELTLFASTAFTHTFGHIYDPADLAAFLAGWN
                     PPERIRDQIADPGWAIALAHDDAGAITGFIKMGPIDFELPEGQPGEAATELHQLYVGE
                     AAKGTGIAVALMDWGIEWARTRASILYLSVFTENPRAQAFYRRYGFVDVGRNAFRVGN
                     HIDEDRIFRLDL"
     gene            584..1351
                     /gene="pgeF"
                     /locus_tag="VN034_13910"
     CDS             584..1351
                     /gene="pgeF"
                     /locus_tag="VN034_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWV61753.1"
                     /translation="MTVSFVTAPSLDGIAHGFFGRRGGVSSGELASLNCGLGSGDDPA
                     LIAANRRRVADAVLPDATLAGLYQVHGNRCMIVDESSDLAARAEADAHVTRTPGILLG
                     ILTADCVPVLFADREAGVVGAAHAGWKGALAGVTDATLDAMESLGANRTHIAAAIGPC
                     IGRASYEVDDGFVQRFLSDDPASERFFAAGRPGHAMFDIAAYVAARLAAAGVTRIAIG
                     GQDTYAEAQDYFSYRRACHKGENTYGRQLSVIGLAGG"
     gene            1359..>2380
                     /locus_tag="VN034_13915"
     CDS             1359..>2380
                     /locus_tag="VN034_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWV61754.1"
                     /translation="MTRTRWLGLIGGLVVFCIMLLLPAPAGMEVTAWRVAALTVLMAI
                     WWMTEALPLTVTALLPFLAVPAFGVMSANAIAKEYYSPILFLILGGAFLALAIERVGL
                     HRRLALALLKRASPTASGLLFAFMTATALLSMFISNTSTTLIMIPIALAVVRAGGVQD
                     GETEGFAGAVMMGIAFAASLGGLGTLVGSPTNAIAAGLIEKALDLRISFLDWAIYGVP
                     IVLLSVPIAMVILARVQRLADHPFDGTAAASALGNQAIWSTAERRVVPIFLLVLIAWI
                     AQPWLEPMLPDGALTDGTIAVAGSLLLFILPDGTGRPLLLWKEADRAPWGVIMMFGGG
                     LALAAA"
BASE COUNT          344 a          857 c          759 g          420 t
ORIGIN      
        1 aaggtcatgg ccgccaccgc ccccggctgg ccgcgacccc aaggtttcgg ggcgatcgag
       61 gaaccggcat gacgaccatc accctggccc gcgacgagga cgtcggcgag ttgacgctgt
      121 tcgcgagcac ggccttcacc cataccttcg ggcatattta cgaccccgcc gaccttgcgg
      181 ccttcctcgc gggctggaac ccgcccgaac gcatccgcga ccagatcgcc gatcccggct
      241 gggcgattgc cctcgcccat gacgacgcgg gcgcgatcac cggcttcatc aagatgggcc
      301 cgatcgattt cgaattgccc gaaggccagc cgggcgaagc tgcgaccgaa ctccaccagc
      361 tctatgtcgg cgaagccgcg aagggcaccg gcatcgccgt cgcgctgatg gactggggta
      421 tcgagtgggc gcgaacgcgc gcatcgatcc tctatctttc ggtcttcacg gaaaatccgc
      481 gcgcgcaggc cttttaccgt cgctacggtt tcgtcgatgt cggccgcaac gcctttcgcg
      541 tcggcaatca tatcgacgag gaccgcatct tccggctcga cctgtgaccg tatccttcgt
      601 taccgcgccg tcgctggacg gtatcgcgca cggtttcttc ggccgccgcg gcggcgtgtc
      661 gagcggcgag ctcgcctcgc tcaactgcgg gctgggatca ggcgacgatc cggcgctgat
      721 cgctgcgaac cggcgacgcg tggccgatgc cgtgctgccc gatgcgacgc tcgctgggct
      781 ttaccaggtc cacggcaatc gctgtatgat cgtcgatgag agcagcgacc tcgccgcgcg
      841 agccgaagcc gacgcccacg tgacgcgcac gcccggcatc ctgctcggca tattgaccgc
      901 agactgtgtc cccgtgctct tcgccgaccg cgaagccggt gtcgtcggcg cggcacacgc
      961 gggctggaaa ggcgcgctcg caggggtcac cgacgcgacg ctggacgcga tggaaagcct
     1021 tggcgccaac cgtacccata tcgccgccgc gatcggcccc tgcatcggcc gcgcctccta
     1081 cgaggtcgac gatggcttcg tgcagcgctt tctcagcgac gatcccgcga gcgaacgctt
     1141 ctttgccgcg ggcaggcccg gccacgcgat gttcgacatc gccgcctatg tcgccgcccg
     1201 gctcgcggcg gccggcgtca cgcgaattgc catcggcggg caggacacct atgccgaagc
     1261 ccaggattat ttcagctatc gccgcgcgtg ccataaggga gaaaacacct atggccgcca
     1321 gctatcggtg atcggactgg cgggaggata aggggtcgat gacgcgcacg cgctggctgg
     1381 gattgatcgg cgggctcgtc gtcttctgca tcatgcttct gctcccggcc cccgccggga
     1441 tggaggtcac cgcttggcgc gtcgcggcgc tgaccgtgct gatggcgatc tggtggatga
     1501 ccgaggcgct gccgctcacc gtcaccgcgc tccttccctt ccttgccgtg cccgcctttg
     1561 gcgtgatgag cgccaatgcg atcgcgaagg aatattattc gccgatcctc ttcctgatcc
     1621 tcggcggcgc cttcctcgcg ctcgcgatcg agcgcgtcgg gctgcaccgg cggctggcgc
     1681 tcgcgctgct caagcgcgcc tcccccaccg catcgggcct gctcttcgcc ttcatgacag
     1741 cgaccgcgct gctcagcatg ttcatctcga acacctcgac gacgctgatc atgatcccga
     1801 tcgcgctcgc ggtcgtgcgc gcgggcgggg tgcaggacgg cgagaccgag ggattcgcgg
     1861 gcgcggtgat gatgggcatc gccttcgccg cctcgctcgg cgggctcggc acactcgtcg
     1921 gcagcccgac caacgcgatc gccgcggggc tcatcgaaaa ggcactggat ctccgcatct
     1981 ccttcctcga ctgggcgatc tatggcgtgc cgatcgtcct gctgtcggtc ccgatcgcga
     2041 tggtcattct cgcgcgcgtc cagcgacttg ccgatcatcc cttcgacggc accgcggccg
     2101 cctcggcgct cggcaatcag gcgatctggt cgaccgccga acgccgcgtc gtcccgatct
     2161 tcctcctcgt cctcattgcc tggatcgcgc agccctggct cgaaccgatg ctgcccgacg
     2221 gcgcgctcac cgacggcacg atcgccgtcg cgggcagcct gctgctcttc attctgcccg
     2281 acggcaccgg ccgcccgctg ctgctctgga aagaggccga ccgcgcaccg tggggcgtca
     2341 tcatgatgtt cgggggcggc ctcgcgctcg ccgccgcgat
//
LOCUS       DATMSU010000522         8871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_47107, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000522 DATMSU010000000
VERSION     DATMSU010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8871
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47107"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VN034_13920"
     CDS             <1..621
                     /locus_tag="VN034_13920"
                     /inference="COORDINATES: protein motif:HMM:NF015541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HWV61755.1"
                     /translation="GGHAALWTAISARSHAPDLTLVATAAAAPPTDLGANLREGSDKN
                     ARALLLSFALHSWSTLYGFSLDPITNRTNQGIINRLAQNNCVEFHKKPKLRTILGILT
                     VARATKGKDIEKIEPFGSFERANSVDPARVPGPLLIAQSSRDTIVAPAVTRKFAKAVC
                     KRGTPTRWIDMTGDHGASAKDSAQETIGWITARFDGNPPPNDCRRI"
     gene            complement(625..1065)
                     /locus_tag="VN034_13925"
     CDS             complement(625..1065)
                     /locus_tag="VN034_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61756.1"
                     /translation="MIKPASIILFDRLFLGSLILGVLNFLFAWNDVSEKLASMPEFAA
                     MGFGSGFIIGSFAVGMIINLIIWYFISARASKIAKWILVVFFAIGLLSMLSNLNNPLG
                     PQGFSLGVAIIITVIQGAAVYMLFRPDAAAWFNRTPPVNPDTFR"
     gene            complement(1089..1982)
                     /gene="era"
                     /locus_tag="VN034_13930"
     CDS             complement(1089..1982)
                     /gene="era"
                     /locus_tag="VN034_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWV61757.1"
                     /translation="MTQHCGFVAVVGAPNAGKSTLVNALVGQKVAIVSPKAQTTRTRL
                     MGVAMDGETQIVLIDTPGIFAPTRRLDRAMVAAAWSSLEEAEAILVMIDAAAKLTGRV
                     ERVLEGIANRPEKKYLILNKVDLTKKDKLLTIATELNAKVAFDETFFIAASSGDGVPE
                     LKAHLAKLMPEGPWHFPEDEVSDAPERMLAAEITREQLYRQLHEELPYQSTVETELFK
                     TRPDGSAEIHQQIFVARDNQRAIVLGKGGARIKEIGARARAELTELMGRKVHLFLHVK
                     VKENWDEDRGVYRDMGLDWVD"
     gene            complement(1979..2659)
                     /gene="rnc"
                     /locus_tag="VN034_13935"
     CDS             complement(1979..2659)
                     /gene="rnc"
                     /locus_tag="VN034_13935"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HWV61758.1"
                     /translation="MSDTLDTGALAGIIGCTPDDLALYDLALTHGSTGRADYQRLEFL
                     GDRVLGLVIASELYMRFPTASEGEMSSRLHVLASGATCAAVAQKLDLTALIRFGAQAR
                     NDGGRYSDNIAADAIEALIGALYLDKGADTARQFILTHWNEMIDGQQAAPKHPKAALQ
                     EWALARKRRPPEYEIVSREGPDHAPRFRVAVSVGKLARAEAEGASKQAAEKAAAAALL
                     AELEGMKE"
     gene            complement(2656..3522)
                     /gene="lepB"
                     /locus_tag="VN034_13940"
     CDS             complement(2656..3522)
                     /gene="lepB"
                     /locus_tag="VN034_13940"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWV61759.1"
                     /translation="MTPPTGPTPPAPTPAPVSAKEEAVDTVRFLALLAIAVLIFRSFF
                     LSPFNIPSESMQPRLLIGDYLLVNKMAYGYSKFSLPFSAPLIPGRIFPRTPERGDVVV
                     FKAPPVNDNDYIKRVIGLPGDSVQVKDGIVWLNGKPLKREAMPDFVIPVTPNMIEASR
                     ATGTLPCYAPEFEEVAADGTRQCRYKQFRETLPSGKSYAILDITTIAEDNTPLVVVPE
                     GHMFLMGDNRDRSADSRFPAMENQGIGLVPEENLVGHALVGMFSTDGSASWINPISWF
                     TAARWGRIGDGF"
     gene            3675..5180
                     /gene="pgi"
                     /locus_tag="VN034_13945"
     CDS             3675..5180
                     /gene="pgi"
                     /locus_tag="VN034_13945"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWV61760.1"
                     /translation="MTTAPDAWQALSDWQPQKLPDLVAADPEARLQVLVRNVADIRFD
                     FAKTHLDGSALAILSGLAETQDFAGRRKTLFSGGIANPTEDRAAEHSAERGDGAPESV
                     HAAQALHQRMRMMIDAIEAGAFGEIRHLLHIGIGGSALGPDLLIDALGRHSDRYDVAV
                     VSNVDGAALDEAFAKFSPEHTLVAVASKTFTTTETLLNANSALQWLDEAGVEDPVGRF
                     IALTAKPERAMEWGIDETRILPFSETVGGRYSLWSSIGFPAALALGWDAFADLLEGAA
                     EMDRHFRLADGADNICLLAAFADQVYANRLGCQTRAVFAYDERLRLLPDYLQQLEMES
                     NGKSVTFEGQAVAQQTAPITWGGVGTDAQHAVFQLLHQGTHLTPVEFVVAREPDHLLD
                     EAHHETLVANCIAQGAALMTGRASEDGARNYPGNRPSTTILLDQVSPRSLGALIAFYE
                     HRVFANAVLLGINPFDQFGVELGKEMAKGLAEGTVEFDAATQALMQAALGD"
     gene            5289..6635
                     /gene="gor"
                     /locus_tag="VN034_13950"
     CDS             5289..6635
                     /gene="gor"
                     /locus_tag="VN034_13950"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HWV61761.1"
                     /translation="MSDFDYDLFVIGAGSGGVRASRIAASHGARVAVAEEYRVGGTCV
                     IRGCVPKKLLVYGAHFAEDIHDARKFGWDVPDCKFDWPVLRDNVLAEVDRLEGLYGQT
                     LDNHKVTVLKTRATVAGPQKIRLADGTELTAERILIATGGWPHVPEFPGSEHAITSNE
                     VFHLETLPKRVVIAGGGYIANEFAGIFNEFGSKVTIVNRGDTILRGYDEQIRDRLLQI
                     SMTKGIDFKFNAPFQSIEKKDDGTLTVHLEGCDAIEADAVLMATGRTPNTKSLGLEEL
                     GVELDDKGAVKVDERNQSSVPSIFAVGDVTDRIQLTPVAIREGQAFADSFYGEKPTVV
                     DYSNVPSAVFSHPPIGAVGMTEAEARNKLGSIRVYTSDFRAMKNVLAGRNERALYKMI
                     VNAATDQVVGLHMIGPDAPEILQAAAIAVKAGLTKADFDATVALHPSMAEELVLLK"
     gene            6675..7691
                     /locus_tag="VN034_13955"
     CDS             6675..7691
                     /locus_tag="VN034_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWV61762.1"
                     /translation="MAGTALVTGGSGYIAGFLIRQLIDNGWTVNTTVRSLAREDEVRG
                     WLNVDDDKLRFFAADLERDAGWAEAMAGCSHVAHVASPFPLGVPKHADELVVPAREGA
                     LRALRFAKAAGVGRFVLTSSMAAIAYGHGKGRELYDEADWTNLDNPAVMPYPRSKTVA
                     ERAARDWVAAEGGGMEFASVNPAAVFGPLLSDDLSTSIELVKQLLEGKVPMCPDIGFG
                     IIDVRDVADLHYRALTAPAIRDERYVCSGPFLKMIDVANILTANLGDKAGKVPTRKMP
                     DWLLKLLALVRPELKQLVAELGNVRGGDSGHAMATLGWTMRPPEEAILATARSLIERG
                     IVKV"
     gene            complement(7795..>8871)
                     /locus_tag="VN034_13960"
     CDS             complement(7795..>8871)
                     /locus_tag="VN034_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HWV61763.1"
                     /translation="VALHAAYRRAGQRLQIIDEALTGGTAEGSRLPWEEVRDWFHDAG
                     NYVDSIDRAAEALAGQLRGKAPSPAIETIERRLRDALGISIVYQQSQVLRDFDATMRH
                     LVIDPSQPAETRRFQLAHQLAALALAKEIAAVVEASPLRTAAARQLLHVGLANYAAGA
                     VLMPYAPFRASARAVRHDIDRLRLEYGVSFEQACHRLSTLQRPGARGIPMFFCRVDMA
                     GNITKRHSATRLQFARFGGACPLWIVHEAAAIPDRILFQLAETPDGLRYVSMAKGLVK
                     PSGSYARSPRRYAVALGCEAQYAADFVYADGVDVAAPQAATRIGPSCRICPRDDCDQR
                     AFPPSDRPILVDPDRRDVVPYRIG"
BASE COUNT         1551 a         2897 c         2946 g         1477 t
ORIGIN      
        1 ggcggccatg cggcgctctg gactgcgatc tcggcgcgca gccacgcgcc cgacctcacg
       61 ctcgtcgcga ccgcggcggc ggcaccaccg accgacctcg gcgccaatct gcgcgaaggc
      121 agcgacaaga atgcgcgcgc actgcttcta tccttcgctc ttcacagctg gtcgacgctc
      181 tacggctttt cgctcgatcc gatcaccaac cggacgaacc agggcatcat caaccggctg
      241 gcgcagaata attgcgtcga gtttcacaag aagccgaagc tccgcacgat cctcggcatc
      301 ctgaccgtcg cccgcgcgac aaaaggcaag gatatcgaga agatcgagcc tttcggctcg
      361 ttcgaacgcg ccaacagcgt cgacccggcg cgcgtgcccg gaccgctgct gatcgcgcag
      421 agcagccggg acacgatcgt cgcgcccgcg gtcacgcgca agttcgccaa agcggtctgc
      481 aagcgcggaa cgccgacgcg ctggatcgac atgaccggcg accatggcgc gagcgcgaag
      541 gacagcgcgc aggaaacgat cggttggatc accgcgcgct tcgacggcaa cccgccgccg
      601 aacgattgcc gccgaatcta gcgtttagcg aaaggtatcg gggttcacgg gcggggtgcg
      661 attgaaccac gcggcggcat cggggcggaa cagcatatag accgccgcgc cctgaatgac
      721 ggtgatgatg atcgctacgc ccagcgagaa gccttggggg ccaagcggat tgttgagatt
      781 gctcagcatc gacaacagcc cgatcgcgaa aaagaccacc aaaatccatt tggcgatctt
      841 gctcgcccgc gccgagatga aataccagat gatcaggttg atgatcatgc cgacggcgaa
      901 tgagccgatg atgaagcccg atccaaagcc catcgccgca aactcgggca tgctcgcgag
      961 tttctccgat acgtcgttcc acgcgaacag aaagttcagc acccccagta taagcgaccc
     1021 cagaaacagg cgatcgaaaa gaatgatgga agccggtttg atcattttta gcccctcgtt
     1081 gctgttgttc aatcgaccca gtcgagcccc atgtcgcgat agacgccgcg gtcctcgtcc
     1141 caattttcct tgaccttgac gtgcaggaac aggtggacct tgcggcccat caactcggtc
     1201 agctcggcgc gcgcgcgggc gccgatttcc ttgatccggg cgccgccctt gccgagcacg
     1261 atcgcgcgct ggttgtcgcg cgcgacgaag atctgctggt ggatttcggc gctgccgtcg
     1321 gggcgcgttt tgaacagctc ggtctcgacc gtcgactggt agggcagttc ctcgtggagc
     1381 tggcggtaga gctgttcgcg cgtgatttcg gcggcgagca tgcgttcggg cgcgtcgctg
     1441 acctcgtcct cggggaagtg ccacggcccc tcgggcatca gcttggcgag gtgcgccttg
     1501 agctccggaa cgccgtcgcc gctgctcgcc gcgatgaaga aggtctcgtc gaacgcgacc
     1561 ttggcgttga gttcggtcgc gatggtgagc agcttgtcct tcttggtcag gtcgaccttg
     1621 ttcaagatca gatatttctt ctcgggccgg ttcgcgatgc cctcgagcac gcgctcgacg
     1681 cggcccgtga gtttggcggc ggcgtcgatc atgacgagga tcgcctcggc ctcctcgagg
     1741 ctgctccatg cggcggcgac catcgcgcgg tcgagccggc gcgtcggcgc gaagatgcct
     1801 ggcgtgtcga tcaggacgat ctgcgtctcg ccgtccatcg cgacgcccat cagccgcgtg
     1861 cgcgtcgtct gcgccttggg gctgacgatc gcgaccttct ggccaacgag cgcgttgacg
     1921 agggtcgact tgcccgcgtt gggcgcgccg acgacggcga cgaaaccgca atgctgggtc
     1981 attctttcat tccttcgagt tcggcgagca gggctgccgc cgcggccttt tcggccgcct
     2041 gtttgctcgc gccctcggcc tcggcgcgcg cgagcttgcc gacgctcacc gcgacgcgga
     2101 agcgcggcgc atggtcggga ccttcgcgcg acacgatttc atattcgggc gggcggcgct
     2161 tgcgggcgag cgcccattcc tggagcgcgg ccttggggtg cttgggcgcc gcctgctgcc
     2221 cgtcgatcat ctcgttccaa tgggtcagga tgaactgccg ggcggtgtcg gcgcccttgt
     2281 cgagatagag cgcgccgatc agcgcctcga tcgcgtcggc ggcgatattg tcgctgtagc
     2341 ggccgccgtc gttgcgcgcc tgcgcgccga aacggatcag cgcggtgagg tcgagctttt
     2401 gcgcgaccgc ggcgcaggtc gcgcccgacg cgaggacgtg cagccgcgac gacatctcgc
     2461 cttcgctggc ggtcgggaag cgcatgtaga gttccgacgc gatgacgagc ccgagcaccc
     2521 ggtcgccgag gaattcgagg cgctgataat cggcgcgccc cgtgctgccg tgcgtcagcg
     2581 caaggtcgta aagcgcgaga tcatccgggg tgcagccgat aatcccggcg agcgccccgg
     2641 tatccagcgt gtcgctcaaa aaccgtcccc gatacggccc cagcgcgcgg cggtgaacca
     2701 gctgatcggg ttgatccagc tcgccgaacc gtcggtcgag aacatgccga cgagcgcgtg
     2761 gcccacgaga ttttcctcgg gaactaggcc gatgccctga ttttccatcg cggggaagcg
     2821 gctgtcggcg ctgcggtcgc ggttgtcgcc catcaggaac atatgcccct cgggcacgac
     2881 gacgagcggg gtgttgtcct cggcgatcgt cgtgatgtcg aggatcgcat agctcttgcc
     2941 gctgggcagc gtttccctga actgcttgta acggcactgg cgcgtcccgt cggcggcgac
     3001 ttcctcgaac tcgggcgcat agcagggcag ggtgcccgtc gcgcgcgacg cctcgatcat
     3061 gttcggcgtc accgggatga cgaagtcggg catcgcctcg cgtttcagcg gcttgccgtt
     3121 gagccagacg atgccgtcct tcacctgcac gctgtcgccg ggcaggccga tcacgcgctt
     3181 gatataatca ttgtcgttga ccggcggcgc cttgaacacg acgacgtcgc cgcgttcggg
     3241 cgtgcgcggg aaaatgcggc cggggatcag cggcgcgctg aacggcaggc tgaatttgga
     3301 gtagccatag gccatcttgt tgacgagcag atagtcgccg atgagcagcc gcggctgcat
     3361 cgattccgac gggatgttga agggcgacag gaaaaagctg cggaaaatca gcaccgcgat
     3421 cgcgagcagc gcgagaaagc ggacggtgtc gaccgcctct tccttggcgg ataccggcgc
     3481 gggagtcgga gcaggcggcg tgggccccgt cgggggagtc acgtcggcgg taaagctggc
     3541 attggtcgtg ctgcggcatt ggcgatgaat aggttgccac gtcaagcaaa tatcggcgga
     3601 cgccactggc gccgcgcggg cggcgggcct agagaaaggt cgcgattcac tccccccttc
     3661 gaggatatac gatgatgacg accgcacccg acgcctggca ggcgctttcc gactggcagc
     3721 cgcagaaact ccccgatctg gtcgcggccg atcccgaagc gcggctgcag gtgctggtgc
     3781 gcaatgtcgc cgacatccgc ttcgacttcg ccaagacgca ccttgacggt tcggctctcg
     3841 ccatcctgtc gggccttgcc gaaacgcagg atttcgcggg ccggcgcaag acgctctttt
     3901 cgggcggtat cgccaacccg accgaagacc gcgccgccga acatagcgcc gagcgcggcg
     3961 acggcgcccc ggaatcggtc catgccgcgc aggcgctgca tcagcggatg cggatgatga
     4021 tcgatgcgat cgaggccggg gcgttcggcg aaatccggca tctgctccac atcggcatcg
     4081 gcggatcggc gctcggtccc gacctgctca tcgacgcgct cggccgtcat tccgatcgtt
     4141 atgacgtcgc ggtggtgtcg aacgtcgacg gcgccgcgct cgacgaagcc ttcgccaagt
     4201 tcagccccga acatacgctt gtcgcggtcg catcgaagac cttcaccacg accgagacct
     4261 tgctcaacgc caattcggcg ctgcaatggc tcgacgaggc gggggtcgag gacccggtcg
     4321 gccgcttcat cgcgctcacc gccaagcccg agcgcgcgat ggaatggggg atcgatgaaa
     4381 cgcgcatcct gccgttcagc gaaaccgtcg gcggccgcta ttcgctctgg tcgtcgatcg
     4441 gcttccccgc ggcgctcgcg ctcggctggg atgcgttcgc cgatctgctc gaaggcgcgg
     4501 ccgagatgga ccgccatttc cgcctcgccg acggcgccga caatatctgc ctgctcgcgg
     4561 ccttcgccga ccaggtctac gccaaccgtc tcggatgcca gacgcgcgcg gtcttcgcct
     4621 atgacgaacg cctccgcctc cttcccgact atctgcaaca gctcgaaatg gaatcgaacg
     4681 gcaagtcggt gacgttcgag gggcaggccg tggcgcagca gaccgcgccg atcacctggg
     4741 gcggggtcgg caccgatgca cagcatgcgg tgttccagct gcttcatcag ggcacccacc
     4801 tgaccccggt cgagttcgtc gtcgcgcgcg agcccgatca tctgctcgac gaggcgcatc
     4861 acgaaacgct cgtcgccaac tgtatcgcgc agggcgcggc gctgatgacc gggcgcgcga
     4921 gcgaggatgg cgcgcgcaac tatccgggca accgcccttc gacgacgatc ctgctcgatc
     4981 aggtcagccc acgcagtctc ggcgcgctca tcgctttcta cgagcatcgc gtcttcgcca
     5041 acgccgtgct gctcggcata aacccgttcg accagttcgg ggtcgaactc ggcaaggaga
     5101 tggcgaaagg cctcgccgaa ggtacggtcg aattcgacgc cgcgacgcag gcgctgatgc
     5161 aggcggcgct cggcgactga aaggcctcgt tgctgactct gagagcaaat ttcgattggc
     5221 aagctgcgcc ggtgtaacca cataggcgcc gggcgcgaat gcctgcctgt ccaattcagg
     5281 ggttatccat gagcgatttc gactatgatc tcttcgtcat cggcgccggt tcgggcggcg
     5341 tccgcgcctc gcgcatcgcc gcctcgcacg gtgcgcgcgt cgcggtggca gaggaatatc
     5401 gggtcggggg cacctgcgtc atccgcggct gcgtgcccaa gaaattgctc gtttacggcg
     5461 cccatttcgc cgaggatatc cacgacgcgc gcaagttcgg ctgggacgtg cccgactgca
     5521 agttcgactg gccggtcctg cgcgacaatg tgctcgccga ggtcgaccgg ctcgagggcc
     5581 tctatggcca gacgctcgac aaccacaagg tcaccgtgct caagacgcgc gcgacggtcg
     5641 ccgggccgca aaagataagg cttgccgacg gcaccgaact caccgccgaa cgcatcctga
     5701 tcgcgaccgg cggctggccg cacgttcccg aatttccggg cagcgaacat gcgatcacct
     5761 cgaacgaggt cttccatctc gaaacgctgc cgaaacgcgt cgtgattgcg ggcggcggct
     5821 atatcgccaa tgaatttgcc ggcatcttca acgaattcgg cagcaaggta acgatcgtca
     5881 atcgcggcga cacgatcctg cgcggctatg acgaacagat tcgcgaccgg ctgctccaga
     5941 tttcgatgac caagggcatc gatttcaagt tcaacgcgcc cttccagtcg atcgagaaga
     6001 aggacgacgg aacgctcacc gtccatctcg aaggctgcga cgcgatcgag gccgatgcgg
     6061 tgctgatggc gaccggacgc acgccgaaca cgaaaagtct cggactcgaa gaattggggg
     6121 tcgaactcga cgacaagggc gcggtcaagg tcgacgagcg gaaccagtcg tcggtgccga
     6181 gcatcttcgc ggtcggcgac gtgaccgatc gcatccagct gactcccgtc gcgatccgcg
     6241 aagggcaggc cttcgccgac agcttctacg gcgaaaagcc gaccgtggtg gattacagca
     6301 acgtccccag cgcggtattc agccacccgc cgatcggcgc ggtgggcatg accgaggccg
     6361 aagcgcgcaa caagctcggc agcatccgcg tctacaccag cgatttccgc gcgatgaaga
     6421 atgtgctcgc gggccgcaac gagcgcgcgc tctacaagat gatcgtcaac gccgcgaccg
     6481 atcaggtcgt cggcctccac atgatcggcc ccgacgcccc cgaaatcctg caagcggcgg
     6541 cgatcgcggt gaaggcgggt ctgaccaagg ccgatttcga cgccaccgtc gcgctgcacc
     6601 cgagcatggc cgaggaactc gtcctgctga aataatcctg ctagcttcgc cgctccgaaa
     6661 caggggaggg gcgcatggcg ggcacggcac tggtcaccgg cggcagcggc tatatcgcgg
     6721 gtttcctgat ccggcagttg atcgacaatg gctggaccgt gaacacgacg gtccgcagcc
     6781 tagcgcgcga ggacgaagtc cgcggctggc tgaacgtcga cgacgacaag ttgcgcttct
     6841 tcgccgccga tctcgagcgc gacgccggct gggccgaagc gatggcggga tgcagccatg
     6901 tcgcgcatgt cgcgtcgcct ttcccgctcg gcgttccgaa gcatgccgac gagttggtgg
     6961 tcccggcgcg cgagggcgcg ctgcgcgcct tgcgcttcgc caaggcggcg ggcgtcggac
     7021 gcttcgtcct cacctcgtcg atggcggcga tcgcctatgg ccatggcaag gggcgcgaac
     7081 tttacgacga agccgactgg accaacctcg ataatcccgc cgtgatgccc tatccgcgct
     7141 cgaagacggt cgccgaacgc gcggcgcgcg actgggtggc ggcggagggc ggcggcatgg
     7201 aattcgcctc ggtcaatccc gcggcggtct ttgggccgct gctctcggac gatctttcga
     7261 cctcgatcga actggtcaaa cagttgcttg agggcaaggt gccgatgtgc cccgacatcg
     7321 gtttcggcat catcgacgtg cgcgacgtcg ccgatctcca ctatcgcgcg ctcaccgccc
     7381 ccgcgatccg cgacgaacgt tatgtctgct cggggccgtt cctgaaaatg atcgacgtcg
     7441 cgaacatcct gaccgcgaac ctcggcgaca aggcggggaa ggtgccgacg cgcaagatgc
     7501 ccgactggct gctcaagctg cttgcgctcg tgcgccccga actcaaacag ctcgtcgccg
     7561 agctcggcaa tgtccgcggt ggcgacagcg gccatgcaat ggcgacgctc ggctggacga
     7621 tgcgcccgcc cgaggaagcg atcctcgcga cggcgcgcag cctgatcgag cgcgggatcg
     7681 tgaaagttta gttcccctcc catttatggg aggggctagg ggagggcatg tttcgaatgt
     7741 cggatacatg acaggccctc ccccgacccc tcccgcaagc gggaggggag aaagttatcc
     7801 gatccggtag ggaacgacgt cgcgccggtc ggggtcgacc aaaatcgggc ggtcgctcgg
     7861 cgggaaggcg cgctggtcgc aatcgtcgcg cggacagatg cggcacgacg ggccgatgcg
     7921 cgtcgccgct tgcggcgcgg cgacatcgac cccgtcggca tagacgaaat cggcggcata
     7981 ttgcgcctcg caccccagcg cgacggcgta gcggcgcgga ctgcgcgcat agctgcccga
     8041 cggcttcacc agccccttcg ccatcgacac atagcgcagc ccgtcgggcg tctcggcgag
     8101 ctgaaacagg atacgatcgg ggatcgccgc ggcctcgtgg acgatccaca gcgggcaggc
     8161 gccgccgaag cgcgcgaact ggaggcgcgt ggcagagtgc cgcttggtga tgttgcccgc
     8221 catgtcgacg cggcaaaaga acatcggaat gccgcgcgcg ccggggcgct ggagcgtcga
     8281 gaggcggtgg cacgcctgtt cgaaactcac gccatattcg agccgcaacc ggtcgatgtc
     8341 gtgccgcacc gcgcgggcgc tggcgcgaaa gggggcgtaa ggcatgagca ccgcgcccgc
     8401 ggcatagttg gcgaggccga catggagcaa ttggcgcgcg gcggcggtgc gcagcggcga
     8461 agcctcgacc accgcggcga tttccttcgc gagcgcgagc gcggcaagct ggtgcgcgag
     8521 ctgaaagcgc cgggtttcgg ccggctgcga cggatcgata acaagatgcc gcatcgtggc
     8581 atcgaaatcg cgcagcacct ggctctgctg atagacgatc gaaatgccga gcgcgtcgcg
     8641 gagtcgccgc tcgatggtct cgatcgcagg cgaaggcgcc ttgccgcgca actgtccggc
     8701 gagcgcctcc gccgcgcggt cgatgctgtc gacataattg ccggcgtcgt ggaaccagtc
     8761 gcgcacctcc tcccacggca gacggctgcc ctcggcggtg ccgccggtca gcgcctcgtc
     8821 gatgatctgc agccgctgcc ccgcgcggcg ataggcggcg tggagcgcga c
//
LOCUS       DATMSU010000523         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_103456,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000523 DATMSU010000000
VERSION     DATMSU010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103456"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VN034_13965"
     CDS             complement(<1..320)
                     /locus_tag="VN034_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HWV61764.1"
                     /translation="MAARPNTPPRPIIVKKVIQEAHGGHHGGAWKVAYADFVTAMMAF
                     FLLMWLLGATNEKQRKALADYFAPTIVQTKTDTAGSTGLFGGDSLVAADRYPHGAAQT
                     GTRA"
     gene            complement(320..1183)
                     /gene="motA"
                     /locus_tag="VN034_13970"
     CDS             complement(320..1183)
                     /gene="motA"
                     /locus_tag="VN034_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HWV61765.1"
                     /translation="MFPVVGIVVLILLVFGGFALTGGNLGPVMHALPHEMLIIGGAAL
                     GSLIIGNSGADLKALAGGLGKVFKGPQYKKQDYLDCILLVSTLMKMMRTEGPVAVEPH
                     IEDPKNSTIFQQYPKLLKDDTLVHLICDTLRLVVVSSGTLDPHAVEDVMDNALKSHHH
                     HSLKPAEGLQSLADALPALGIVAAVLGVVKTMGSIDQPPAILGAMIGSALVGTFLGVL
                     LAYGLVGPFATRAKTVIESDGAIYHTVKQLIIASLHGHPQPLVIEAARSGVDHHNQPS
                     FAEVFDGMRGR"
     gene            complement(1301..>1787)
                     /locus_tag="VN034_13975"
     CDS             complement(1301..>1787)
                     /locus_tag="VN034_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HWV61766.1"
                     /translation="DDLGELDALAATRDSNGEPVFAAGGARVMRFDSDVTFAPVPSAA
                     DVFVVGGNSLSTGIRDAAAAVAANDKAGMNASLGALDTAISHVADEHAKIGLSGGRLD
                     RIGDSLASRGITLKDERSVVEDTDLSVAIAELNAQELTLSAAQAAFAKINRQTLFDIL
                     S"
BASE COUNT          307 a          583 c          596 g          301 t
ORIGIN      
        1 atcgcgcgcg tgcccgtctg cgcggcgcca tgcggatagc ggtcggccgc caccagcgaa
       61 tcgccgccga acaggcccgt cgaccccgcg gtgtcggttt tcgtctgcac gatcgtcggc
      121 gcgaaatagt cggcgagcgc cttgcgctgc ttttcgttcg tcgcgccgag caaccacatc
      181 agaaggaaga aggccatcat cgcggtgacg aagtcggcat aggcgacctt ccacgcaccg
      241 ccgtgatggc cgccatgcgc ttcctgaatc accttcttga cgatgatcgg gcgcggcggg
      301 gtgttcgggc gggcagccat cagcgacctc tcatcccgtc aaagacttcg gcgaagctcg
      361 gctggttgtg atggtcgacg cccgagcgcg ccgcctcgat caccagcggc tgcggatggc
      421 cgtggagcga ggcgatgatc agctgcttca cggtgtgata gatggcgcca tcgctttcga
      481 tcacggtctt ggcgcgcgtc gcgaacggcc cgacgaggcc ataggcgagc agcacgccga
      541 ggaaggtgcc gacgagcgcc gagccgatca tcgcgccgag gatcgccggc ggctggtcga
      601 tcgagcccat cgtcttgacg acgccgagca ccgccgcgac gatgccgagc gcggggagcg
      661 cgtcggcgag gctttgcagg ccctcggcgg gcttcaggct atgatgatga tggctcttca
      721 gcgcattgtc catcacgtcc tcgaccgcgt gcgggtcgag cgtccccgac gacacgacga
      781 cgaggcgcag cgtgtcgcag atcaggtgga caagcgtgtc gtccttcaaa agcttgggat
      841 attgctggaa gatcgtcgaa tttttcggat cctcgatgtg cggctcgacc gcgaccggcc
      901 cttcggtgcg catcatcttc atcagcgtcg agacgagcag gatgcagtcg agatagtcct
      961 gcttcttgta ctggggtccc ttgaacacct tgccgagtcc gccggcgagc gccttcaggt
     1021 cggcgcccga attgccgatg atcagcgagc caagcgccgc gccgccgatg atcagcatct
     1081 cgtgcggcag cgcgtgcatg acggggccaa ggttgccgcc ggtcagcgca aagcccccga
     1141 agaccagcag gatcaagacg acgatgccga caacgggaaa catgtgtgcg caactccatt
     1201 cgggacccgg ctgtcgaggc cggcccccgc aaactcaggc cccactaact cagggacgtc
     1261 tggatgatta acggcagcgc cgccgcaaga ttgagggccg tcagctcaaa atatcgaaca
     1321 aggtctggcg gttgatcttg gcgaaggctg cctgcgcggc gctgagcgtg agttcctggg
     1381 cgttgagttc ggcgatcgcg accgacagat cggtatcctc gaccaccgag cgttcgtcct
     1441 tgagcgtgat cccgcgcgac gcgaggctgt cgccgatgcg gtcgaggcgc ccgcccgaca
     1501 ggccgatctt ggcatgttcg tcggcgacgt ggctgatcgc ggtatcgaga gcgccgagcg
     1561 atgcgttcat tcccgccttg tcgttcgccg cgaccgccgc cgccgcgtcg cggatgcccg
     1621 tcgacaggct gttgccgccg acgacgaaca catcggccgc cgagggcacg ggcgcgaagg
     1681 tgacgtccga atcgaaacgc atcacgcgcg ctccgcccgc ggcgaatacc ggctcgccgt
     1741 tcgaatcgcg ggtcgccgcc agcgcatcga gctcgccgag atcgtcc
//
LOCUS       DATMSU010000524        10447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_56368, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000524 DATMSU010000000
VERSION     DATMSU010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56368"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VN034_13980"
     CDS             complement(<1..572)
                     /locus_tag="VN034_13980"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWV61767.1"
                     /translation="MADRFAFQISATDGAARTGVIAMQRGAIRTPAFMPVGTAATVKA
                     MRPAEVRATGADIILGNTYHLMLRPTAERMARLGGLHNFMGWDRPILTDSGGYQVMSL
                     SALTKQSEEGVAFKSHLDGSRHMLTPERSMEIQRLLGSDIVMAFDECPPSGVDAKRAE
                     ASMERSMRWAARSRAGFDAGEEHAARSALFG"
     gene            complement(772..1260)
                     /locus_tag="VN034_13985"
     CDS             complement(772..1260)
                     /locus_tag="VN034_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWV61768.1"
                     /translation="MTETRIFARLKADHDRHRELLDRIDATHGDSDDRRALFEAFRVE
                     VTAHAASEEMSLYATMLANPDLRDEAQHSVAEHKEIEDFLTELYEMDFSSSGWLTRFR
                     TMKHRYLHHIDEEEEEMFPAAEKELSDARKKELIKIFEKEKPKEKAKAAEEEPSSEEA
                     RD"
     gene            complement(1321..3510)
                     /locus_tag="VN034_13990"
     CDS             complement(1321..3510)
                     /locus_tag="VN034_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWV61769.1"
                     /translation="MDVPSRIDSGDRGQEDADEAQRGFFGKLGSRIGKPRGEAAANLH
                     THDALLLLRNYEESGQGWFWSTDAKGRLTYITDAVARLMGRTGGALLGTALTDLFLPV
                     DSQGERQRTLPFLLTKQSKFHELPLRSAFEGDDRWWAISGRPQFDGSGKFTGYRGSGT
                     DITEQRRSAEDASRLALYDSLTGLANRFNISKKLDATLAAFAQQQRACAIMLIDLDRF
                     KQVNDTLGHPAGDALLKQVAQRLLKIVGDREMVSRLGGDEFQIILPDIEDRGKLGEMA
                     TDIIASLSQPYSVEGSRCIIGASVGVAIAPFDGQGSDDLVRNADLALYAAKGNGRGRF
                     AFYSSDLHQAAEDRRMLEEDLRDALARGEIALCYQPVVNSKSNMVTGVEALIRWTHRD
                     RGVISPAVFIPIAEEANLIWKLGEWVLRRACEDAASWPGDMRVAVNVSPIQFANADLP
                     KVVAQAIEASGLAPDRLELEITESVFLGDTAETTRMFKALKDLGVRLALDDFGTGYSS
                     LGYLQSAPFDKIKIDQSFVRDATVPGSRNGAIIAAIVALAEALEMETTAEGIESLDQL
                     DLIRKLHVSHVQGYVYSKPVPHAELMEHAEAGSWTIKPSGPARQRNDRFSLFRKVGAI
                     HDNHRYAVVIRNLSTTGAFIEGILDVPVGTRFVIDFGEGQLVTATVRRSAKHQQGIEF
                     EQTMVSDGNGGLCTRHRVSPYLIAAAAQQTSAMALPTFTTTGDWKAA"
     gene            3731..4396
                     /locus_tag="VN034_13995"
     CDS             3731..4396
                     /locus_tag="VN034_13995"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61770.1"
                     /translation="MAMTVYLNDVPRSAGKGRASRRQLRLPLHGSKATGAEIEALVHN
                     ISATGMLVESGAALEIGEVIEVNLPHSGKTATKVIWTSGRLAGCQFEMPISPATLSAA
                     QLRSVVGADAHPAEDLPAPATESFGVRLQRLRTAKELTQGQLAVRLGVSEPSISAWEL
                     DKARPKAGRIEALSEALGVEISELLGFDEAENLNDLVAKAKAQIAKAAGVDPDKIKFT
                     IEI"
     gene            4518..4937
                     /locus_tag="VN034_14000"
     CDS             4518..4937
                     /locus_tag="VN034_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61771.1"
                     /translation="MKKARLYGAIACISIAFTSNGAMAAEAGKASFDVSVTVPEYCEI
                     NASTVLTSQGNGFASGTVFESCNTQEGFQVVASHRPLEQNETVAFNYAGELTYLREEG
                     WSQVANRTGAKYGLRPISVQYSGLATPLAINLTITTI"
     gene            complement(4961..5845)
                     /locus_tag="VN034_14005"
     CDS             complement(4961..5845)
                     /locus_tag="VN034_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61772.1"
                     /translation="MRAYMGMPIALALASFATAAQAECVPSFVAGGNSVALTPSGSFE
                     DGQLNERFHVRLRNDGDTICELRLAVGRDIAASDPNFPKFELFGPNGAVAISDADSAR
                     SSARAAVDIVIPTGGETSIAYDVRMKLGWGFESGTYAQALEFFLLPRSGQGELGVQRT
                     QLSLNIPAIARIRFAGAGSARGAASVEMGTLSPNAPSTSPPFAIRVLSTAGYQMDLQS
                     ENMGALRRIDGPEVIPYRMTVSGRAMNLRGGGDRIQVASHTSSTGNVHPVSVVVDPDP
                     TFHAGNYRDRITVTVTPM"
     gene            complement(5860..8277)
                     /locus_tag="VN034_14010"
     CDS             complement(5860..8277)
                     /locus_tag="VN034_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61773.1"
                     /translation="MALQSPHAYAAMQDQGPTPPPAAAAQRWTQLQLPLVRNGQLSGD
                     IIAEVTPDGQIRFERASLLERLTPYLTPSGAETLATSFAGATFLTPEELAAAGVVLRY
                     NPSLLEVAVERIDANLVAVQTLGPVITESRVPVTQQPEDFSAYLNVVGDFRLEDFETF
                     EDPGVLVFGAARFKGVVVEFDGGYDRAITGGRGGFYRRSARAVYDEVDKLRRWSAGDL
                     QLSGIPVISGALLGGVAVEKGRRVFSGIGPVAQLGSQQILLERDATVEVIVDGQQVQT
                     LQLAAGPYDLSQLTSQYGGRNAQLFVTDVTGRRQLASFDPFFDPVDLGAGEDEYSAGI
                     GFVAKAFEASPKYGGLPAFSGYYRRGMSNRLLLGGALQLSEDVQVAGAEVVVSPQNIP
                     GRIEFSGAISTGDDFGYSLRGAYSLQIGRGTQGRQLSVSADYRSDGFQTLTDAIAGDR
                     FETLNVTANYAQSLSERTTLVTGANWFHRPGFNSSHTVYADVIHRTQRFRVTAGVEYG
                     KGLNENRFGVRLGVTVPLGGRTRAEANYNSRRETARAFVTRSYEDRVGSFGYDVGVRR
                     SRNDATIDAIANYVGNRFYARGSVTTGGPGFGEIDQRQQARLQVGTSIAFAGGDVAIG
                     RPINDSFVIAKPHESAEPGQVVLGRSVQDKRFDALSGTLGPALGSRLQSYTRQNVTYD
                     LINGAPGYDIGSGIDTVEPPYRSGYNITVGSDANVTAYGVLSIDGAPASLIAGSISST
                     DDEDFASQPFFTNSVGRFAIMGLRPGKTYQVRLTDPAGSYVIEVPADSQPLYQLGDIA
                     ITSAAAK"
     gene            complement(8349..9143)
                     /locus_tag="VN034_14015"
     CDS             complement(8349..9143)
                     /locus_tag="VN034_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HWV61774.1"
                     /translation="MFRKSMMLALAAASLALSANPAEAARVTPMTVEMTPAGRGSTAR
                     IEVTNSEDRTLPVELRMYRGDVSETGELTLTPADEKFVVFPPQVVIGPNAQQVFRIQY
                     LPSEPLAKSEIYYAAVSQIPVELDSTVSRIQVVMRFNVLVNVVPEGSKPDPVVTSAKA
                     LVRETVVPPDERLPVEEQKPKTVTERGIEVRIENRGTRYFAAGRTGWTINATREDGTA
                     FSESRTASRMSDAVGFGLVPPGGARIFFVPVDEPLREEGVSVTLGE"
     gene            complement(9240..9791)
                     /locus_tag="VN034_14020"
     CDS             complement(9240..9791)
                     /locus_tag="VN034_14020"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HWV61775.1"
                     /translation="MNKLLIASLGAMLTATPAFAADSDSESFDITGNVAETCTMEGIN
                     DVELGTLSIDTTAGSGALFINNTSAEETDPFWVSCNESNRMLIEADLGVLKNQTRTLQ
                     AGDDAGFKDTINYSVRALNYFNSGAGPEFNSANGSTGQNTVRGAIHRQVELRARVRAN
                     ENTDLRPLAGEYRDTVTVTVTTI"
     gene            complement(9880..10413)
                     /locus_tag="VN034_14025"
     CDS             complement(9880..10413)
                     /locus_tag="VN034_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61776.1"
                     /translation="MAALSAMLSTPAFAADSDSESFDITANVAETCTMEGISDIELGA
                     LSINTNAGSTALFLNGLSADNAGEFWLSCNESNRMSIVPTLGRLKNQTRSVQPGDDNG
                     FKDTINYSVAALNYRNGFLQPSFNSLTGPLFQNLQRGAIHRKIDMGAVVTGLDNFDAR
                     PLAGTYKDTVTVTVTTI"
BASE COUNT         1855 a         3375 c         3341 g         1876 t
ORIGIN      
        1 ccgaacagcg ccgagcgcgc cgcgtgctcc tcgccggcat cgaagcccgc gcggctgcgc
       61 gccgcccagc gcatcgagcg ttccatgctc gcctcggcgc gcttcgcatc gacgccgctc
      121 ggcgggcatt cgtcgaaggc catcacgatg tcgctgccga gcaggcgctg gatttccatc
      181 gagcgttcgg gggtcagcat gtgccgcgag ccgtcgaggt ggctcttgaa cgcgaccccc
      241 tcctcactct gcttggtcag cgccgacagg ctcatcacct gatagccacc gctgtcggtc
      301 aggatcggcc ggtcccaacc catgaaattg tggaggccac cgagccgcgc catgcgctcg
      361 gcggtcgggc gcagcatcag gtgataggtg ttgccgagga tgatgtcggc ccctgtcgcg
      421 cggacctcgg cggggcgcat cgccttcacc gtcgcggcgg tgccgacggg catgaaggcg
      481 ggggtgcgga tcgcgccgcg ctgcatggcg atcacgcccg tgcgcgcggc accgtcggtc
      541 gcgctgatct gaaaggcgaa tctgtcggcc atggcgcgcg cctatggcgg ggatgcgagc
      601 gaaagtcgag atgggcggca aaaggcggcg ttgcgctcaa gacattcccc tccctgcaag
      661 ggaggggtca ggggtgggtg cgagcgtagc gagccgaaag ggagaacacc gcctgcggcg
      721 gctacccacc cccagcccct cccttccagg gaggggaggt caaacgccgc cctaatcccg
      781 cgcctcttcg ctcgacggct cctcttcggc cgccttcgct ttttccttgg gcttttcctt
      841 ttcgaagatc ttgatcagtt ccttcttgcg cgcgtcggaa agctcctttt cggccgccgg
      901 gaacatctcc tcctcttcct cgtcgatatg gtggagatag cggtgcttca tcgtccggaa
      961 ccgcgtcagc catccgctcg acgaaaaatc catctcgtat aattcggtga ggaaatcctc
     1021 gatttcctta tgctcggcga cgctgtgctg cgcctcgtcg cgcaggtcgg gattcgcgag
     1081 catcgtcgca taaagcgaca tttcctccga cgccgcatgc gcggtcactt cgacccggaa
     1141 cgcctcgaac agcgcgcggc gatcgtcgct gtcgccatgc gtcgcgtcga tgcggtcgag
     1201 caattcgcga tggcggtcgt gatcggcctt cagccgcgcg aaaatcctgg tctcggtcat
     1261 atgtgtctcc gtccgtttgg gtcgaagaca aaacggggca ggccgcgacg ggttccggcg
     1321 tcaggccgct ttccagtcgc ccgtcgtcgt gaaggtcggc agcgccattg cgctcgtctg
     1381 ctgcgccgcc gcggcgatca gatagggcga gacacgatgg cgcgtgcaca gcccgccatt
     1441 gccgtcgctg accatcgtct gttcgaattc gatgccctgc tggtgctttg ccgagcggcg
     1501 cacggtcgcg gtgacgagct gcccttcgcc gaaatcgatg acaaagcgcg taccgacggg
     1561 cacgtccaaa atgccctcga taaaggcgcc ggtggtcgac aggttgcgga tgacgacggc
     1621 gtaacggtga ttgtcgtgga tcgcgccgac cttgcggaac agcgagaaac ggtcgttacg
     1681 ctggcgcgcc ggcccggacg gcttgatcgt ccacgatccc gcctcggcat gctccatcag
     1741 ctcggcatgc ggcaccggct tgctatagac atagccctgg acgtggctga cgtggagctt
     1801 gcggatcagg tcgagctggt cgagcgattc gatgccctcg gcggtcgttt ccatctcaag
     1861 cgcttcggcc agcgcgacga tcgcggcgat gatcgcgccg ttgcgcgatc ctggcacggt
     1921 ggcgtcgcga acgaagctct ggtcgatctt gatcttgtcg aacggcgccg attgcagata
     1981 gccgagcgac gaatagccgg tgccgaaatc gtcgagcgca aggcggaccc ccagatcctt
     2041 cagcgccttg aacattcggg tcgtttcggc ggtgtcgccg aggaacacgc tttcggtgat
     2101 ttccagttcc agccggtcgg gcgcaaggcc cgatgcctcg atcgcctggg cgacgacttt
     2161 cggcaggtcg gcgttcgcga actggatcgg cgagacattg accgcgacgc gcatgtcgcc
     2221 cggccagctc gccgcatcct cgcacgcccg gcggagcacc cattcgccca gcttccagat
     2281 cagattggct tcctcggcga tcggaatgaa caccgcgggc gatatcacgc cgcggtcgcg
     2341 gtgggtccag cggatcagcg cctcgactcc cgtcaccatg ttcgatttgg aattgaccac
     2401 gggctgatag caaagcgcga tctcgccgcg cgccagcgcg tcgcgcaaat cctcttcgag
     2461 catgcggcgg tcctcggccg cctgatgcag atcgctcgaa tagaaggcga agcggccgcg
     2521 tccgttgccc tttgccgcat aaagcgccag atcggcgttg cggacgaggt cgtcgctgcc
     2581 ctgtccgtcg aagggcgcga tcgcgacgcc gaccgacgcg ccgatgatgc agcggctgcc
     2641 ctcgaccgaa tagggctgcg acaggctggc gatgatgtcg gtcgccatct cgcccaattt
     2701 gccgcgatcc tcaatgtcgg gaaggatgat ctggaattcg tcgccgccga ggcggctgac
     2761 catttcgcgg tcgccgacga tcttgagcag gcgctgggcg acctgtttca gcagcgcatc
     2821 gcccgccgga tggccgagcg tgtcgttgac ctgcttgaac cggtcgaggt cgatcagcat
     2881 gatcgcgcac gcgcgttgct gctgcgcgaa cgccgcgagc gtcgcgtcga gctttttcga
     2941 gatgttgaag cggttggcga gaccggtgag cgaatcgtag agtgcgagcc gcgatgcgtc
     3001 ctcggccgag cggcgctgtt cggtgatgtc ggtgccgctt ccccggtagc cggtgaattt
     3061 gcccgaaccg tcgaactgcg gccggcccga gatcgcccac cagcgatcat cgccttcgaa
     3121 cgcgctgcgc agcggcaatt cgtggaattt cgactgtttg gtcagcagga agggcaaagt
     3181 gcgctggcgt tcgccctggc tgtcgacagg caggaacagg tcggtcaaag cggtgccgag
     3241 cagcgcgccg ccggttcgcc ccatcagccg cgccaccgca tcggtgatat aggtcagccg
     3301 ccccttggca tcggtcgacc agaaccagcc ttgcccgctc tcttcgtaat tgcggagcag
     3361 caagagtgcg tcgtgcgtgt gcagattcgc cgcggcctcg ccgcgcggtt tgccgatgcg
     3421 ggagccgagc ttaccgaaaa agccgcgctg ggcttcgtcg gcatcctcct gtccccggtc
     3481 gccggaatcg atccgcgatg gcacgtccat ccaacaaact ctttatctga tccccactgc
     3541 ctggccgcta tgcgcggcaa cagattgcga tagtgtaaat atccgagagc ttgccccaaa
     3601 gcgaaacatc tgaacgattt gggccgcctc cccgcacgcg gcgttgcgcc ctcgcgactt
     3661 tcactcatcc tgcctcatta acctttccac gaagccaaat gttaatatgg gcctcgcgat
     3721 gggtgcgcgt atggcaatga ctgtatatct gaacgatgtc ccccggtcgg cgggcaaggg
     3781 ccgcgcgtcg cgccggcaac tgcggctgcc gctgcacggg tcgaaggcga cgggcgcgga
     3841 aatcgaggcg ctggtccata atatttcggc gacgggaatg ctcgtcgaaa gcggcgcggc
     3901 gctggaaatc ggcgaggtga tcgaggtcaa cctgccgcac agcggcaaga cggcgacgaa
     3961 agtcatctgg acgagcggcc gccttgccgg atgccagttc gaaatgccga tctcgcccgc
     4021 cacgctcagc gccgcgcagc tccgcagcgt ggtgggggcc gacgcgcacc ccgccgagga
     4081 tctgcccgct cccgccacgg aaagcttcgg cgtccgcctg caacggctgc gcacggcaaa
     4141 ggaattgacc cagggccagc tcgcggtccg gctgggagtc agcgagccgt cgatctcggc
     4201 atgggaactc gacaaggcac gccccaaggc cggccggatc gaggcgctgt ccgaggcgct
     4261 cggcgttgaa atctccgaac tgctggggtt cgacgaggcc gaaaatctga acgatctggt
     4321 cgcgaaggcc aaggcccaaa tcgccaaggc ggcaggcgtc gaccccgaca agatcaagtt
     4381 caccatcgag atttgaaccg ccgccggacc ccacgcttcg caaaaccagc ggtaattcct
     4441 gccattttcg gctgaaaatc aaacattcct taactcgcgt cgcgtaggga actctcagca
     4501 gaagagggtg aaagacgatg aagaaggctc ggctttacgg cgcgatcgca tgtatttcga
     4561 tcgccttcac cagcaacggc gccatggcgg ccgaagccgg caaggcgtcg ttcgacgtga
     4621 gcgtcaccgt tcccgaatat tgcgagatca acgcctcgac ggtgctgacc tcgcagggca
     4681 atggctttgc cagcggtacc gtattcgaaa gctgcaatac gcaggaaggc tttcaggtcg
     4741 tcgcgtcgca ccgcccgctc gaacagaatg aaacggtcgc cttcaactat gccggcgaat
     4801 tgacctatct tcgcgaagaa ggctggagtc aggtcgccaa ccgcaccggc gccaaatacg
     4861 gcctacgccc gatcagcgtc cagtatagcg gcctcgcgac gccgctggcg atcaacctta
     4921 cgatcaccac gatctgaccc gccgcggcgg gcaggcattc ttacatcggc gtgaccgtga
     4981 ccgtgatccg gtcgcgataa ttgcccgcat ggaatgtcgg gtcgggatcg acgacaaccg
     5041 agaccgggtg aacattgccc gtcgagcttg tgtggctcgc cacctgaata cggtcgccgc
     5101 cgccgcgcag gttcatcgcg cgtcccgaca ccgtcatgcg atagggaatg acctcggggc
     5161 cgtcgatccg gcgcagcgcg cccatatttt ccgactgaag atccatctga tagcccgcgg
     5221 tgctgagcac gcgaatggca aagggcggcg atgtcgacgg cgcgttcggc gacaaggttc
     5281 ccatctcgac gctggcagcg ccccgcgcgc tgcccgcgcc ggcgaaacga atgcgcgcga
     5341 tggccggaat gttgaggctg agctgggtgc gctgcacgcc cagttcgccc tgcccgctcc
     5401 gcggaagcag gaagaattcg agcgcctggg cgtaggttcc cgattcgaag ccccagccca
     5461 atttcatgcg gacgtcatag gcgatcgatg tctcgccccc ggtcgggatc acgatgtcga
     5521 ccgccgcgcg cgcgctcgac cgcgcggaat ccgcgtcgct gatcgcgacg gcgccgttcg
     5581 gcccgaacag ctcgaacttg gggaaattcg gatcggatgc ggcaatatcg cggcccactg
     5641 caagccgcag ctcgcatatc gtgtcgccgt cgttacgcag ccggacgtgg aaacgctcgt
     5701 tcaactggcc gtcctcgaag ctgcccgacg gcgtgagcgc gaccgaattg ccaccggcga
     5761 cgaagctcgg cacgcactcc gcttgcgcgg cggtagcgaa actcgccagc gccagcgcga
     5821 tcggcattcc catatatgcg cgcatccatc ccactcctgt catttcgccg ccgccgacgt
     5881 gatcgcgatg tcgcccagct ggtacagcgg ctgcgaatcg gcgggcacct cgatcacata
     5941 gctgccggcg ggatcggtca atcgcacctg gtaggtcttg cccgggcgca gacccatgat
     6001 agcaaatcgg cccaccgaat tggtaaagaa tggctggctc gcaaaatctt catcgtcggt
     6061 cgacgaaatc gacccggcga tcagcgatgc cggcgcaccg tcgatgctga gcacgccgta
     6121 agcggtgaca ttcgcgtcgc tgccgacggt gatattatag ccgctgcgat agggcggctc
     6181 gacggtgtcg atcccgctgc cgatgtcata acccggggcg ccgttgatca gatcataggt
     6241 cacattctgc ctcgtatagg attgcaggcg cgaaccgagc gcggggccaa gagtcccgct
     6301 gagcgcgtcg aaacgtttat cctgaaccga ccggccgagc acgacctggc ccggttccgc
     6361 gctttcgtgc ggcttggcaa tgacgaaact gtcgttgatc gggcgaccga tcgccacgtc
     6421 gccgcccgcg aaggcgatcg acgtgccgac ctgaagccgg gcctgctggc gctggtcgat
     6481 ctcgccaaag cccggcccgc cggtggtgac cgagccgcgc gcatagaagc ggttgccgac
     6541 ataattggcg atcgcatcga tcgtcgcgtc gttccgcgac cggcggacgc cgacgtcgta
     6601 accgaaggat ccgacccggt cttcatagct gcgcgtgacg aaagcgcgcg cggtttcgcg
     6661 ccggctgttg taattggcct cggcgcgggt gcggccgccc agcggcaccg tcacgcccag
     6721 ccggacgccg aagcgattct cgttcaatcc cttgccatat tcgacgcccg cggtgacgcg
     6781 gaacctttgg gtgcggtgga tgacatcggc atagacggtg tgactgctgt tgaacccggg
     6841 ccggtggaac cagttcgccc ccgtgacgag cgtcgtccgt tcgctgaggc tttgcgcata
     6901 attggcggta acgttcagtg tttcgaaccg gtcgcccgcg atcgcatcgg tcagcgtctg
     6961 gaagccgtcg ctgcgataat cggcgctcac cgacaattgc cgcccctgcg tcccccttcc
     7021 gatctggagg ctgtacgcgc cgcgcagcga atagccgaaa tcgtcgcccg tgctgatggc
     7081 gcccgaaaat tcgatccggc cgggaatatt ctggggcgac acgacgactt cggcgcccgc
     7141 aacctgcacg tcttccgaca attgcagcgc gccgcccagc aacaggcggt tgctcatgcc
     7201 gcggcgataa tagcccgaga aggccggcaa tccgccatat ttcggcgaag cctcgaacgc
     7261 cttcgcgacg aaaccgatgc ccgcgctata ttcgtcttcg cccgccccca ggtcgaccgg
     7321 atcgaaaaag ggatcgaacg aggccagctg ccgccggccg gtcacatcgg tgacgaagag
     7381 ctgcgcgttg cggccgccat attgcgaggt cagctgcgac aggtcgtagg ggccggcggc
     7441 cagttgcaag gtctgcacct gctggccgtc gacgatcact tcgaccgtcg cgtcgcgttc
     7501 aagcaatatc tgctggctgc ccaattgcgc gacgggaccg atgcctgaaa acacccgccg
     7561 tcccttttcg acggcgacgc cgccgagcag cgcgcccgat atgacgggaa tgcccgacaa
     7621 ttgaaggtcg cccgcgctcc agcggcgcag cttgtcgacc tcgtcataga ccgcgcgcgc
     7681 cgaacggcga tagaagccgc cccgcccgcc ggttatcgcc ctgtcataac cgccgtcgaa
     7741 ttcgaccacg acgcccttga agcgcgcggc gccgaacacc agcacgcccg gatcctcgaa
     7801 cgtctcgaaa tcttcgaggc ggaaatcgcc gaccacgttc agataggcgc tgaaatcctc
     7861 gggttgctgc gtcaccggca cgcggctctc ggtgatcacg gggcccagcg tctgcaccgc
     7921 gacgagattg gcatcgatcc gctcgaccgc gacttcgagc agcgacggat tatagcggag
     7981 gacgacgcct gcggcggcaa gctcctcggg ggtcaaaaag gtcgccccgg caaaggatgt
     8041 ggcgagcgtt tcggcgcccg acggagtaag gtagggcgtc aatcgttcga ggagcgacgc
     8101 gcgctcgaag cgtatctggc cgtcgggggt cacttcagcg atgatatcgc cgctcaactg
     8161 gccgttgcgg acgagtggca gctgtaactg cgtccagcgc tgcgccgcgg cggcgggagg
     8221 cggggtgggc ccctggtcct gcatcgcagc ataggcatgg ggcgactgaa gcgccaccaa
     8281 cgccaatgtg atgcgtgaac aatagcgtat cactgaaccc ccctgaactg ccccgcgtcg
     8341 ctcctcgatt attccccgag cgtgacgctg acgccttcct cgcgtagcgg ctcatcgacc
     8401 ggaacgaaga aaatgcgcgc ccccccgggc ggaacgagac caaagccgac cgcgtcgctc
     8461 atccggctcg ccgtgcgcga ttcggaaaag gcggtaccgt cctcgcgcgt ggcattgatc
     8521 gtccagccgg tgcgcccggc ggcgaagtag cgcgtgccgc gattttcgat gcgcacctcg
     8581 atgccgcgtt cggtgaccgt cttcggcttc tgctcctcga ccggcagccg ttcgtcgggc
     8641 ggcaccaccg tttcgcgaac cagcgccttt gcggaagtga cgacggggtc gggcttgctg
     8701 ccttcgggca cgacattgac gagcacattg aagcgcatca ccacctggat ccgcgaaacc
     8761 gtcgaatcga gttcgaccgg aatctggctg accgcggcat aatagatttc ggatttggcg
     8821 agcggctcgc tcggcagata ctgaattcgg aaaacctgct gcgcgttcgg cccgatcacc
     8881 acctgcggcg gaaagacgac gaacttctcg tcggccgggg tgagcgtcaa ctcgcccgtc
     8941 tcgctcacat cgccgcggta catccgcagc tcgaccggca aggtgcggtc ttcggaattg
     9001 gtgacctcga tgcgcgcggt cgaaccgcgg cccgccgggg tcatttcgac cgtcatcggc
     9061 gtcacgcgcg cggcctctgc ggggttggcg ctcaaggcca ggcttgcggc ggcgagcgcc
     9121 agcatcatag atttgcggaa caaggccaat cccccccggg tcgcttgcag aaagtatcgc
     9181 tacagactga aagggggagc cttaagctcc cccttccgca tctgcttaac gaaaacctat
     9241 cagatcgtcg tcacggtgac cgtgacggtg tcccgatatt caccggccag cggacggaga
     9301 tcggtgtttt cgttggcacg aacgcgcgcg cgcagttcga cctggcgatg gatcgcgccg
     9361 cggacggtgt tctggccggt cgaaccgttg gccgaattga attccggacc ggcgccgctg
     9421 ttgaaatagt tgagcgcacg gaccgaataa ttgatcgtgt ccttgaagcc ggcgtcgtcg
     9481 cccgcctgaa gcgtgcgcgt ctggttcttc agcacgccga ggtcagcctc gatcagcatg
     9541 cggttgctct cgttgcagct gacccagaac ggatcggtct cttcggccga ggtgttgttg
     9601 atgaacagcg cgcccgaacc ggcggtggtg tcgatcgaca gcgtgccgag ttcgacatcg
     9661 ttgatgcctt ccatcgtgca ggtttcggca acattgccgg tgatgtcgaa cgattcgctg
     9721 tccgaatcgg cggcgaaagc gggcgtcgcg gtgagcatgg caccaagcga agcgatcaga
     9781 agcttgttca tggaattatt cctccaggcg atcggatcgc ggacatcgcg acccgcaaag
     9841 aagcagggga agggcggcgc cctccccctg cgatcaggat cagatcgtcg tgacggtgac
     9901 ggtcacggtg tccttgtagg tgccggccag cgggcgggca tcgaagttgt cgaggcccgt
     9961 gacgacagcg cccatgtcga tcttgcgatg gatcgcgccg cgctgcaggt tctggaacag
    10021 cgggccggtc aggctgttga acgacggctg caaaaagccg ttgcgatagt tcagggccgc
    10081 gaccgaatag ttgatcgtat ccttgaaccc gttgtcatcg cccggctgca cgctacgcgt
    10141 ctggttcttg agcctgccga gcgtcggaac gatcgacatg cggttgctct cgttgcagct
    10201 cagccagaat tcgccggcgt tgtcggccga caggccgttc aggaacagcg cggtgctgcc
    10261 ggcgttggtg ttgatcgaca gcgcgccgag ttcgatgtcg ctgatgcctt ccatcgtgca
    10321 ggtttcagca acgtttgcgg tgatgtcgaa cgattccgaa tccgaatccg ccgcgaaagc
    10381 aggcgtcgac agcatggccg acagcgcggc cacagcaaaa agcttcttca tggtaattcc
    10441 cctcaat
//
LOCUS       DATMSU010000525         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_27526, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000525 DATMSU010000000
VERSION     DATMSU010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27526"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="VN034_14030"
     CDS             <1..358
                     /locus_tag="VN034_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA thiolase"
                     /protein_id="HWV61777.1"
                     /translation="VAFAESGGDPAASLTAGFAAMDKVLARAGLTLGDIDRIEFMEAF
                     AVTIAKFHRDRDADPGRVNVSGGHLAKGHPMGASGAILTSTLLDALDACGGRYGLVVL
                     TGAMGVGAAMVVERVA"
     regulatory      566..786
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            964..2823
                     /locus_tag="VN034_14035"
     CDS             964..2823
                     /locus_tag="VN034_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61778.1"
                     /translation="MLKFTTISLIALAVATPALAETAGGERGDDIVVTASGIEQSRDE
                     VGQAITIIDADTIERRQSVDVVDLLATTPGVRFNRSGSTGSVASISLRGAETTQTLVL
                     IDGVKVNDPSGIGDGYDFGHLLTGNIDRIEVLRGSNSVVHGSQAIGGVVSLMTATPAD
                     GFAANASAEYGYSDTLNAKADVSGTAGAISGGIGGAYFRTDGISSAAVGTEKDGYRNF
                     AGNARLKVAFSDALSLDLRGYYINADLDYDSFFGAPADSADVAKLDQYVGYAGLNLGL
                     FDGNFTSRAAVTWMRNDRDYYFVRGTAPDYGYSGTNLRFEYQGVVTPAAQAKLIFGYE
                     HERPDYDFFGFGSTDSQKANIDSVYALGIVQPLAGLSLSGGVRHDDHSQFGGATTLGA
                     NANYSPNAGATNFRLSYGEGFKAPSLYQLYDAFSGNAALRPERSKSYDLGIDQSLADG
                     RALISLTAFLRNTVDQINYDNATFTYGNIDRTRAKGIEATLALKPVDALNVSASYSII
                     DARDRSGRPAFDGKRLPRRAAHAVSLSADYDWSFGLSTGATLTMVGDSFDDAANAVPL
                     DGYALAGIRASFPVGDNFEITGRVDNLFDANYATAFNYGTYGRAAYGGVRARF"
     gene            2823..3431
                     /gene="cobO"
                     /locus_tag="VN034_14040"
     CDS             2823..3431
                     /gene="cobO"
                     /locus_tag="VN034_14040"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWV61779.1"
                     /translation="MKARSDAEHTAKMKKIQAAQAKKVAAKTVEKGLVIVHTGPGKGK
                     TSAALGMAVRAIGHGMKVGVIQFVKGAMATGEKAVFDRFPELIEFKPMGEGFTWDTQD
                     RTRDIAVARAAWEEVKRMIADPSYAMVIADELNIVLRYDYLPVDEVLEALSAKPHMTH
                     VIITGRNAPQPLIDAADLVTEMAQVKHPFREQNVKAQKGIEF"
BASE COUNT          615 a         1126 c         1222 g          597 t
ORIGIN      
        1 ggtcgccttt gccgagagcg gcggcgatcc ggcggcgtcg ctgaccgcgg ggtttgccgc
       61 gatggacaag gtgctggcgc gcgccgggct gacgctcggc gacatcgacc ggatcgagtt
      121 catggaggcg ttcgcggtca ccatcgccaa attccaccgc gaccgcgacg ccgatccggg
      181 ccgggtcaat gtgtcgggtg ggcatctcgc caagggccat ccgatgggcg cgagcggggc
      241 gatcctgacc tcgaccctgc tcgacgcgct cgatgcgtgc ggcgggcgat acgggctcgt
      301 cgtgctgacc ggcgcgatgg gggtcggtgc ggctatggtg gtcgagcggg tggcttagaa
      361 ccaactcctc cctgtggcgg agccatgggg aggtggcagc ccggagggct gacggagggg
      421 ctatagcgca accgtcgcgg cccctccacc atccgcttcg cggacggtcc ccctccccac
      481 ggcttcgccg cagggaggat cgtttctgca aatcccgatt gcgcaaaatc gccgttccgc
      541 tatgggcggc gcgtgcgggg cttgccccgc gcatgatcgc gccggtgccc gagagggctt
      601 aaaatgggaa cgcggtaacg gggttcgtcc tgaaccccca aagccgaggc tgtccctgca
      661 actgtaagcg gcgagcgaga tgcaccggtc cgaagcgttc ggatcagcca ctggggtcac
      721 ctgggaaggc gtgcatcgag ccctgacccg cgagccagga gacctgccgg cgtgggtcgc
      781 tcaatgccaa ggcccaggga aaggccgcgg cacggaggtt tccgtcgagc gacgaccagg
      841 cggctggagc cgccctggtg ccggagtcgc aggcctttac gccacttgcg acgccagcat
      901 cggccggtcg cgcccgcgca tcggcaggct ccgccttcgt tgccggcgtg cgggggcctt
      961 cccatgttga agttcacgac tatttcactg atcgcattgg ccgtggcgac gccagccctt
     1021 gccgaaacgg cgggcggcga acgcggcgac gatatcgtcg tcaccgcgtc gggcatcgaa
     1081 caatcgcgcg acgaagtcgg ccaagcgatc acgatcatcg acgccgacac gatcgaacgg
     1141 cgccagtcgg tcgatgtcgt cgacctgctc gccaccacgc ccggcgtgcg tttcaaccgg
     1201 tcgggcagca ccggatcggt ggcgagcata tcgctgcgcg gcgccgaaac gacgcagact
     1261 ttggtgctga tcgacggggt gaaggtcaac gacccgagcg gcatcggcga cggatatgat
     1321 ttcggccatc tgctgaccgg caacatcgac cggatcgaag tgctgcgcgg gtcgaactcg
     1381 gtcgtccacg gcagccaggc gatcggcggc gtcgtcagcc tgatgaccgc aacgcccgcc
     1441 gacggcttcg cggcgaatgc ttcggccgaa tatggctata gcgacacttt gaatgccaag
     1501 gccgatgtat cgggcaccgc cggagcgatt tcgggcggga tcggcggcgc ttattttcgc
     1561 accgacggca tttcgtcggc ggcggtcggc accgagaagg acggatacag gaatttcgcc
     1621 ggaaacgcgc ggttgaaggt cgcgttcagc gacgcgctga gcctcgacct gcgcggctat
     1681 tacatcaacg ccgacctcga ttatgacagc ttcttcggcg cgcccgccga cagcgccgac
     1741 gtcgccaagc tcgaccaata tgtcggctat gccgggctga acctcggcct gttcgacggc
     1801 aatttcacca gccgcgccgc ggtgacgtgg atgcgcaacg accgcgatta ttatttcgtg
     1861 cgcgggaccg cgcccgatta cggctattcg ggcaccaatt tgcgcttcga atatcagggc
     1921 gtcgtgacgc cggcagcgca ggcgaagctg atcttcgggt atgagcacga gcgccccgat
     1981 tatgatttct tcggcttcgg atcgaccgac agccagaagg ccaatatcga cagcgtctat
     2041 gcactgggga tcgtccagcc gcttgccggg ctgtcgctgt cgggcggcgt ccgccacgac
     2101 gaccatagcc agttcggcgg cgcgacgacg ctgggcgcca acgccaatta ttcgcccaat
     2161 gcgggcgcga ccaatttccg gttgagctat ggcgaggggt tcaaggcgcc gtcgctctat
     2221 cagctttatg acgcgttcag cggcaatgcc gcgctgcgcc ccgagcggtc gaaaagctac
     2281 gacctcggca tcgaccagag cctcgcggac ggccgcgcgc tgatctcgct gacggcgttc
     2341 ctgcgcaaca cggtcgacca gatcaattac gacaatgcca ccttcaccta tggcaatatc
     2401 gaccgcacgc gcgccaaggg gatcgaggcg acgctggcgc tgaagcccgt cgacgcgctg
     2461 aacgtcagcg cgtcgtacag cattatcgac gcgcgcgacc ggtcggggcg ccctgcgttt
     2521 gacggcaagc gcctgccccg ccgcgccgcg cacgcggtca gcctgtcggc cgattatgac
     2581 tggtcgttcg ggctgtcgac gggtgcgacg ctgacgatgg tcggcgacag tttcgacgat
     2641 gcggcgaacg cggtgccgct cgacggctat gcgctcgccg gaatccgtgc ctccttcccg
     2701 gtcggcgaca atttcgagat taccggccgc gtcgacaatc tgttcgacgc caattatgcg
     2761 acggccttca attacggcac ctatggccgc gcggcctatg gcggcgtgcg ggcgcgcttc
     2821 tgatgaaggc gcggagcgac gccgagcata cggcgaagat gaagaagatc caagcggcgc
     2881 aggcgaagaa ggtcgcggcg aagacggtcg aaaagggtct cgtcatcgtc cacaccggcc
     2941 ccggtaaggg caagacctcg gcggcgctcg gcatggcggt gcgcgcgatc gggcacggga
     3001 tgaaggtcgg cgtgatccag ttcgtgaagg gcgcgatggc gaccggcgag aaggcggtgt
     3061 tcgaccgctt tcccgagctg atcgaattca agccgatggg cgaaggcttt acctgggata
     3121 cgcaggaccg gacgcgcgac atcgcggtcg cgcgcgcggc gtgggaggag gtcaagcgga
     3181 tgatcgccga tccctcctat gcgatggtga tcgccgacga gctcaatatc gtgctgcgtt
     3241 acgattatct gcccgttgac gaagtgctgg aggcgctgag cgcgaagccg catatgactc
     3301 atgtgatcat caccggacgc aacgcgccgc agccgctgat cgacgccgcc gatctggtca
     3361 ccgagatggc gcaggtgaag catccgtttc gcgagcagaa tgtgaaggcg caaaagggga
     3421 ttgagttttg atgcggcggc gcggcctttt ggcggcgacg gtgctgctct cgggtagcgc
     3481 ggggctgctg tgggcaacct cccccgcccc gcctgtgaag gcgcctgcgg tgccgcagcg
     3541 catcgtgtcg atcaacctct
//
LOCUS       DATMSU010000526        22178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_75302, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000526 DATMSU010000000
VERSION     DATMSU010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 22178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22178
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75302"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..723
                     /locus_tag="VN034_14045"
     CDS             79..723
                     /locus_tag="VN034_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61780.1"
                     /translation="MIPGKRPSARPVRRRPRSGEKPAPPPRKADLRRADLVRAAIALL
                     IDRGLPDSPVDDIAAHAGVAKGTFYHYFASKADLMEALRAHFIDDFQARVVAAVDARP
                     VGDWNGRFEAWIVAATDSYFDMHALHDLVFHGPSMPHRQSMGDADIVRHLAALLAAGR
                     AAGVWSLGNEEPIQVAALIFHGLHGMVDDALASGALSDRMGTRIARLTRRMLAS"
     gene            complement(891..2294)
                     /locus_tag="VN034_14050"
     CDS             complement(891..2294)
                     /locus_tag="VN034_14050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61781.1"
                     /translation="MTIGAETPQEQGLEARRIASTFRVLAVVQSTLIFTIALIMIPLP
                     AIAVEFGLGVADVLLLQIAYGLPFSGLLLFGGRLADRFGGRPMFLASLAAFGAASLGA
                     AVASDFHLLVGMRFIQGAAAALAAPAAMAILRLLFRDQADYSRAMATWGGVSVLGAVV
                     GFVTSGILITWLSWRWMFAVPVVVSLFGILAISRLTRGAGHHEPVRPGLDPGGALLAT
                     AGIVASSVGFIASHSYHWGSVAVIGPLGAGLALLAVFLFAETKVRDPLLPPSFLCSSR
                     RAVGLVGMLFAAAGSLLIEFVLLFYLQTAKGWSPLATSLAFLPFVGCILLSNGLAARL
                     TMRIGAARVIAAGSATGACGLLLLALLDRETTYLAVLLPAQILLAVGLSLLFSGSAVM
                     ATSNVADNEGGLAGGVLNTAMELGPTIGFSALMAIAATQSDAIRGYAWAFGAAGIGYL
                     CLAAMAGAMASRRFGQP"
     gene            complement(2291..2833)
                     /locus_tag="VN034_14055"
     CDS             complement(2291..2833)
                     /locus_tag="VN034_14055"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HWV61782.1"
                     /translation="MAELGWTIFVGVGATIAMDLWARLLRGIFGVASLDYAWVGRWIG
                     HMAQGRFAHERIAAAPAIAGERSLGWAAHYAIGVAFAGLLLFMVGAGWARQPTPWPAI
                     AIGVASLAAPFFIMQPAFGLGIAAARLPNPNVARFRSLLTHLIFGVGLYASALLFASM
                     SPHYFSAGEPQTISMQEIIR"
     gene            2918..3376
                     /locus_tag="VN034_14060"
     CDS             2918..3376
                     /locus_tag="VN034_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61783.1"
                     /translation="MKLIDIAELARRSGVPASALRYYEEKGLIQSVGRFGLRRTFAPA
                     TLQQLSLIALAQAGGFSLQEIGEMFAPCGTPRIDRAQLEAKAEEIERTISRLTATQTA
                     LRHVAACPASNHFECPSFSRMLRLAARGRAQPRANRTKPLRHRMGDSRNR"
     gene            complement(3740..4666)
                     /locus_tag="VN034_14065"
     CDS             complement(3740..4666)
                     /locus_tag="VN034_14065"
                     /inference="COORDINATES: protein motif:HMM:NF040209.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5829 family protein"
                     /protein_id="HWV61784.1"
                     /translation="MKIVIALALLAVGGFPTLSAFHANSRDDENSRGGSAVASQSTPS
                     LNHVYIVLDQSTFDAIRDSRTLARLFGRADAGLPDYALPPPEADRIFFRGRRTYLELF
                     APNNRFEEPVGKVGLALGHDHAKDFEELATKWKDACGSRFRRTSIEYRRQQPPVPWYE
                     AIQCDDTASGPHLAVWSMVYRPEFHEWQSGGIGSSSQRIARAEILAPRRADGQGRFDI
                     TGLSLKVSATTYPNLSRQLEQAGMVRDGDQFLGDGWKIKLEVDETPRFVEINFDTDAS
                     RVRGERLGNAVLTRASNRAARLTVRARGRPGQ"
     gene            4887..6977
                     /locus_tag="VN034_14070"
     CDS             4887..6977
                     /locus_tag="VN034_14070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HWV61785.1"
                     /translation="MFRSFLLAGAALGVPAAALAQSEADAEIVVLGTREQGYRATVAP
                     QTNKSDTPLKETPYSVQVVTRELIRDRGITTIGEALRYVPGFSPQVGFGASNDRFYVR
                     GFITPYNLKNGLRRSAYAPDEQLQNVEQVEVLKGSASALYGRFEPGGVVNFVTKKPLE
                     ESFAEVSALYGSFDQLRLTGDFSVPLSETLGLRVNISHDDRDGFRDFTFSRDTFIAPV
                     IQWKPSDATSVTLEGEYARKRGYFDRGFANDPLFLEAPRERQFGEEDARYTNKGGVAS
                     LFVDHRFSDALSGRIAFGYSDFTKDSYYYAFGFPPVSRADPADPKVNRRTSLAYDRQT
                     DLTAQAELYARFATGAVQHKALFGVEYGYDHWRFDVRNPPFGVNIPIDFDNPVYGQRA
                     EPSVLVADGAWNSDSTAIYAQDELKLGNWRLLVGGRYDWNELRNRDYLAGTGEGETKR
                     GAFSPRAGLTWTPVPALSLYGSWARSFRAQVDVGRLRDGSLPKPLIGESWEVGAKAEL
                     FGGTLTPTLALFDITRRNVAVSDPVDFDLVIQIGKSRSRGIEVEIPAVLTPQWRLLAN
                     YTYLDAKILEDSFATPGARLVNAPRHSASLWTTYDFAGSLKGASAGFGIQHIGSRAGN
                     TDNSIVLPAYTRVDANLAYEFDAGFGPLRAQLNVLNLFDKFYYDSGGAFIPLYPGAPR
                     TVTASLSYRFGGGR"
     gene            6974..8212
                     /locus_tag="VN034_14075"
     CDS             6974..8212
                     /locus_tag="VN034_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61786.1"
                     /translation="MRLHLLAMGLLATLAACAPIPMSEPPTRRAMAVGERPAANLAAG
                     CVADYRADTDYFPDKAAFAYSAQLRVEYHGHWKRVTFRPSVDTREVLRLVLVQCGTPP
                     PKLGPRDTLVEIPVRDFATANVSLLGASELLGIDDRLKGVPTRNVSIPAIAARIESGE
                     TIPIYAAQHGNGEQAAALGADLFFTFYSAYPDANIHPLLKRLGVVSAPQADHTEPTPL
                     GRAEWIKYLALFFNLEKRANQLFDERAARYVELRALTANVEARPLVQLGYPESRDEWS
                     QAGGRNQLYRMIEDAGGRHAWHDNAIAGSLTYAPMEKLFDRAAGAEVWLGNFMPGAAD
                     MATLRRDHPRLTWLRAVQGGRVYWFDAGKVGPFANSWSDQGMTEPQDALAELIAILHP
                     ELLKPPAKQRFIRKIPGGAA"
     gene            8209..9243
                     /locus_tag="VN034_14080"
     CDS             8209..9243
                     /locus_tag="VN034_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWV61787.1"
                     /translation="MIRPVSFAIVIGVVIVALFVAELALGVVDIPFEAIGASLTGGQI
                     AQEGWGHIVREYRLPRALAALVGGASLGACGLMLQTLFRNPLADPFVLGIAHGARFAV
                     ALLVVATGLAGPAFAESFGLVGDVGLAVAASVGTMIIMGLLTLLARRVDNVTLLLSGL
                     MLGYIAAGLISLSLHLIDETQASAFKGWDDASFAGVTWAQHIILLPACALGLLLLMAL
                     AKPLNILMLGDTYAQSLGVPLKALRYACFGVVALLVGTITAFCGAINFLGLIAGHAAR
                     GVLQTEDHRILLPAAALAGGALALATDLITHLPWARHLLHLNAVNGLIGGPIVLWVLL
                     RRHARFGAAA"
     gene            9240..10199
                     /locus_tag="VN034_14085"
     CDS             9240..10199
                     /locus_tag="VN034_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWV61788.1"
                     /translation="MSAILSTDGIAIGYPGRLVADHLSLDLHPGDVVALVGPNGTGKT
                     TLLRTLAGLLAPLAGSITLDGDPLASLSAREKAKKLGVVLTERLQTGYLTGFELAAAG
                     RYPHTGWQGRLAHEDIAIIDEALALVGAAAFRDRAVASLSDGERQRVMVARALAQQPR
                     LLLLDEVTSFLDVPRRVAFFALLGELAGQRGYAIVISTHELELALRCANRLWLYDGTK
                     LLDGAPEDLILSGDFARIFAADAVSFDEHAGHFELRRPANRRVALDGDGVRGAWTRRA
                     LERAGYAVDAASALRVSVDAAGWSVGSERADSIAAMLGRLDRS"
     gene            complement(10373..11329)
                     /locus_tag="VN034_14090"
     CDS             complement(10373..11329)
                     /locus_tag="VN034_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61789.1"
                     /translation="MIIKESWKYHLLPFFFLAGLTVSPAPSAGSPPTLSAAARPISEI
                     AVGASSFEAALPGGETIKVFTYRAENHGPDDPIIILLAGGGRNGADYRDSWIRAAERF
                     RLLILAPSFDEAQFPGPINYNLAGMVREDADVATLRNVVLEQPETWLFSDMEAIFDQA
                     VARTGSRQTRYDLFGHSAGAQIVHRMILFAPETRVRTAVAANSGWYTVPAQNVSFPYG
                     LGGISLPGEQLEKAFGRRLVLLLGERDDAHETRGHLRSSPEANAQGPHRLARGEYFHD
                     LATSEATRLGLLLNWRLGMIPGVGHDYRRMGEAAAHQIYGTP"
     gene            11413..12222
                     /locus_tag="VN034_14095"
     CDS             11413..12222
                     /locus_tag="VN034_14095"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWV61790.1"
                     /translation="MSEFVVNGPPGSRRDMTLHLGGIAALTLVLVTSGAFDSDDLPLA
                     RRLLMFGVISGLLVVQASVIADVARRYSGSSLIETATAAVFSLVTTLLLMTIEVHLLK
                     ATVVVPYEPDPLPDFALFLAPFVIPVGALVLCIKWFGVARRDSPSVSSVGSLTPPEPC
                     DTSPPMPALIENWPREPILRIRAADHYLEIWTAGGISLVRGRMQDALRRVSRTAGIQP
                     HRSWWVSITEIDRIERRGRDWFLMMKDGESLPVARGRAAAVRLSLEDQRTD"
     gene            complement(12268..12462)
                     /locus_tag="VN034_14100"
     CDS             complement(12268..12462)
                     /locus_tag="VN034_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61791.1"
                     /translation="MPDSLKADGRERRLAPIGGARFVAPDAYVPAGTLQLAFKRNAFG
                     RVESLVVSGGGELLFDRAFP"
     gene            12503..12916
                     /locus_tag="VN034_14105"
     CDS             12503..12916
                     /locus_tag="VN034_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61792.1"
                     /translation="MAQLAPKGQQFSLVRRWYKVARLDEKDWKYGSLSCCGRRLTEYA
                     ELAVLELYERGGCRSGRQTILELTPTGPVNRGVIYLNVQRGQKNGHTESQSGRIENVV
                     IGKSFDVVVPHTDVRERYEVSSGRYFGPAKSRLRC"
     gene            complement(13121..14191)
                     /locus_tag="VN034_14110"
     CDS             complement(13121..14191)
                     /locus_tag="VN034_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HWV61793.1"
                     /translation="MAADNPPINLGRTLGLALVLASDAPLLLLDENLAVVAASDSFCA
                     AFGIDPETVTGQTIYDLDHHHWDLPRLHSLIEATLSGAAEVEAYEMEIKTSAGAVRCV
                     VIKAQRLDYGDGLNTRMMVTVIDVTEERQNEKRQKDLVHANAALLHEMQHRVANSLQI
                     IASILMQSARKVQSDEARGHLSDAHQRVMSIATLQRHLAGSDGETVELEPYLAQLCAS
                     IGASMIYDREQLTLTTDVDPVSISGDVSVSMGLVVTELVINALKHAFPDGRPGHIVVG
                     YVAQGDDWTLSVSDDGVGMDEDTERSTAGLGTRIVQALAKQLGAEIAVEDTAPGTRVS
                     LTRTVRIARQVAANDEAQIAAV"
     gene            complement(15044..15301)
                     /locus_tag="VN034_14115"
     CDS             complement(15044..15301)
                     /locus_tag="VN034_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61794.1"
                     /translation="MADEIHTTREPDGTTHTTTVIDRDPKRGGGAGITLLVVAALAIA
                     AFLGFQFLGNEKAETGAVTEAAQKVGDAAQDVGDAAQEAVK"
     gene            15622..18390
                     /locus_tag="VN034_14120"
     CDS             15622..18390
                     /locus_tag="VN034_14120"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWV61795.1"
                     /translation="MALGASANLSVQSEAVLREQYASLVRQVPLMYALMFLNVLFLAL
                     VTADGESWAISFVAPLALTLIIAVRGATWYARRGALATDDHIRLYLRGTVFRAGAFSL
                     LFGGWGLYLFLAADPFHTTAIALFIFVGSISCCYCLQALPAAARLVLLFGAMPVTLGL
                     LVSRDWYFTGMGLTFLLGAGVIVRTIATTHSAVFESLRSRSEMFALIEALRRSEEHYR
                     FSVELNPQIPWISDPEGQIVEVGPRWTELTGVPPEDALGRGWTESLHPDDLPVVLAHW
                     NDALTSVDNAVADVRYRVRNIDGSYRWCRARANPRRDADGRIVSWYGTLEDIHDQVMA
                     ELALRESEERYRLASHATNDVIWDWSHRTNRIEWGNGAVDVLGYPEASNGTAVSWWKE
                     RVHPDDLPGVEATYDKVLRNLQDSWSHEYRFRAAQGNFINLFSRGYVVRDDEGHAVRS
                     IGALLDVTAARRAEEELRWAANHDPLTGLPNRKLFAEVLEQALTAAAANASYVCVIVI
                     DVDGFKLLNDSMGHAAGDELLRTIARGLQGNLPAEATVARLGGDEFAVIVPGLAPDSP
                     RASAVDAVLKGIADTLTIDESVLPVSISAGAAVWPTDGDDAEDILKSADLALYAAKAE
                     GAGVVREFRPEMRESAESRKSMLREAREALRDDRVIPFYQAKVDLRSGDVIGFEALLR
                     WHHHRRGLQPPDSIQAAFDDSLLASELTDRMIDRVLADVGEFNEKGVAFGRIAINGSL
                     ADFRRDDFADRILGKFQRAGLPPTLLELEVTESVFVDHLAFNVERALRTLEAEGVSIA
                     LDDFGTGYASLTHLQQFPVHVLKIDRSFISRLDSTDSADFAIVHGVIDIAHRMNIVTV
                     AEGVESDNQLVRLRSLGCDIAQGYLFSRAISAERVPAWLRKWAQDPPLWLEAATDRAT
                     ADPPTRSA"
     gene            complement(18815..19204)
                     /locus_tag="VN034_14125"
     CDS             complement(18815..19204)
                     /locus_tag="VN034_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWV61796.1"
                     /translation="MTTAIPPHRCSVLVVEDEWLLAMDLVDALEAAGFDVKQTDAGED
                     ALRTLGNGGAIDVLVTDIRLAGDMTGWDLAEAYRSLRPQGGVIYASANAPLPHRQVAG
                     SAFYAKPAAMSELIDDCQRLCAAQYPE"
     gene            19352..20908
                     /locus_tag="VN034_14130"
     CDS             19352..20908
                     /locus_tag="VN034_14130"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWV61797.1"
                     /translation="MADGNERAARPDSEIATHHLAAIVESSEDAIISKDLNGIVTSWN
                     KAAERLFGFSPAEMVGQSILTLIPDERRDEEDLILGRIRSGDRVDHFETVRQRKDGSL
                     VDISLTVSPIRRADGTVIGASKIARDISERRSTEAKLAEQARTLSQLNRVATIVSQDL
                     DLERIVQSVTDIATELSGARFGAFFYNVLDETGESYLLFSLSGAPRDAFERFGMPRNT
                     AVFDPTFRGEGVLRSDDIRLDARYGHNDPHHGMPEGHLPVVSYLAVPVVSSSGKVHGG
                     LFFGHDEPGKFDEGIEELIVGIAGQAAVAMDNAHLHEAARFEIAQRARTEEALELLLG
                     ELRHRVKNTLAMVQAIASQTFLAAPAEERRSFEARVRALSDAHDLLTEKNWGIVSVGE
                     MIERALHVFVKVHPGRIAIDGPDASFPASKALLVAMALHELGTNAVKYGALSNTAGTV
                     DVSWELITEGAHQFLILTWVEAGGPPVTPPTRHGFGSRMVERALRSQRGNAEIEFRPE
                     GVRCVLRLPA"
     gene            complement(20945..21124)
                     /locus_tag="VN034_14135"
     CDS             complement(20945..21124)
                     /locus_tag="VN034_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61798.1"
                     /translation="MEWGIMTIIGPILLVLVLAWAMLNNRRSAAEKRRTEEATRMRRA
                     EEEEAEKARDPNNAD"
     gene            21292..21516
                     /locus_tag="VN034_14140"
     CDS             21292..21516
                     /locus_tag="VN034_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61799.1"
                     /translation="MTRIPDNIWAAMTAAEDAYGEGAEAFARGKAVEAEAGGNTEQAS
                     EWHLVADELHILHAINRQWVRPRSTSRPST"
     gene            complement(21606..21875)
                     /locus_tag="VN034_14145"
     CDS             complement(21606..21875)
                     /locus_tag="VN034_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61800.1"
                     /translation="MTDIDAELLFHLPLGEIERIVFYKRDEVTADLICCDVKVAGKTW
                     MFHEALPGWDLLLGHLGALPGFRSDAIAAIAHPPFEKSQAVAYAR"
BASE COUNT         4110 a         6914 c         6917 g         4237 t
ORIGIN      
        1 gtttgaatta gacgggtcaa gagggggtcg catcgtcgat gcgaatgctg ctaaaccccg
       61 gctgtgtcag gcagagatat gatcccggga aaaaggccct cggcgcgtcc ggtacgcaga
      121 cgcccccggt ccggcgagaa gcctgcgcct cctccgcgca aggccgacct gcgccgcgcc
      181 gaccttgtgc gggcggccat cgcgctgttg atcgatcggg gactgccgga cagtccggtc
      241 gacgacatcg ccgcgcacgc aggcgtcgcg aaggggactt tctaccatta tttcgcgagc
      301 aaggcggatc tgatggaagc gctgcgcgcg catttcatcg atgattttca ggctcgcgtc
      361 gtcgctgccg tggatgcccg ccccgtcggt gactggaacg gccggttcga ggcctggatt
      421 gtcgcggcga ccgatagcta tttcgatatg cacgcgttgc atgacctggt gtttcacggc
      481 ccgagcatgc cgcaccgcca gtcgatgggc gatgcggata tcgttcgcca tcttgctgca
      541 cttctggccg cgggaagggc ggcgggcgtc tggtcccttg gcaacgaaga gccaatccag
      601 gtcgccgcgc tgatctttca cgggctgcat ggcatggtcg acgacgcctt ggctagcggt
      661 gccctctcgg accggatggg cacccggatc gcccggctta ctagacgcat gctcgcgagc
      721 tgaaccacct gttttgtctt gaaattctgg caaggtatgc tccaaattac gactcgtgga
      781 gcatctccat aaaagctcaa gccatgttga ggtaaagcga taaaatgccg gtagaggatt
      841 gcgcacgcga agtgcgggca aataaatcct gatggcgcca atatcgccct tcacggttgc
      901 ccaaaccttc tcgacgccat ggcgcccgcc atcgccgcaa ggcacagata gccaatcccg
      961 gcggcgccaa aggcccaggc atatccccgt atcgcgtcgg actgggtggc ggcgatcgcc
     1021 atgagcgccg aaaagcctat ggtcggccca agctccatcg ccgtgttcaa gacgccgccc
     1081 gcaagcccgc cctcattatc ggcgacgttc gacgtcgcca tgacggccga gcctgaaaac
     1141 aggagagaca agccgaccgc gagcaggatc tgggccggga gcagtactgc cagataggtc
     1201 gtttccctgt cgagcagggc caaaagcaac agcccgcacg ccccggtagc cgatcccgcc
     1261 gcgatcacgc gcgccgcacc tatccgcatc gtcagccgcg cggccagccc gttgctcaaa
     1321 aggatgcacc ccacgaaggg aagaaaggcg agagacgtcg ccagcggtga ccagcccttg
     1381 gcggtctgga gatagaaaag cagcacaaac tcgatgagca gcgatcccgc cgccgcgaaa
     1441 agcatgccga cgaggccgac cgctcgccgc gaactgcaca gaaaactggg cggcagcaaa
     1501 gggtcccgca ccttcgtctc ggcgaacaga aaaaccgcta gcaacgccag cccagctccc
     1561 agaggaccaa tgacggcaac cgaaccccag tggtagctgt ggctggcaat gaagccgacg
     1621 ctactcgcca cgatccccgc cgtcgcgagg agcgcgccgc cgggatcaag accaggcctc
     1681 accggctcgt gatgcccggc gcccctggtt aggcgagaga tggcgaggat accgaagagc
     1741 gagacgacaa caggcacggc aaacatccag cgccacgaca gccatgttat gaggatgccc
     1801 gaagtaacga aaccaacgac ggcgccgagg accgaaaccc cgccccaggt cgccatggcg
     1861 cgactgtaat ctgcctggtc gcgaaaaagg agacgaagga tcgccattgc ggcgggcgcg
     1921 gccagcgcgg cggccgcgcc ctgaatgaaa cgcataccaa cgagcagatg gaaatcggac
     1981 gccacggcgg cgccgagaga cgccgctccg aatgccgcca gcgaagcgag gaacatcggc
     2041 cgtccgccaa acctatccgc cagacgcccg ccaaaaagca aaaggccgct gaaaggcaga
     2101 ccgtaggcaa tttggaggag caacacatcc gcaaccccca acccgaattc gacggcaatt
     2161 gccggcagcg gaatcatgat taacgcgatc gtgaagatca aggtcgattg aacgaccgcg
     2221 aggacgcgga acgtcgaggc tatccgccgc gcttccaggc cttgctcttg cggcgtttct
     2281 gctccgatcg tcatcgaata atctcctgca tcgatatagt ttgaggttcg cccgccgaaa
     2341 aataatgcgg cgacatggat gcgaagagca gcgccgaagc ataaagaccg acgccgaata
     2401 tgaggtgcgt gaggaggctg cggaatcggg cgacattcgg gttcggcagt ctggcggcgg
     2461 cgatcccaag accgaaggcc ggctgcatga tgaagaacgg agcggcgagc gaggctaccc
     2521 cgatggcgat cgctggccat ggggtcggct gccgcgccca gccggcaccg accatgaaca
     2581 agagcaggcc ggcaaatgcg acgccgatcg cataatgcgc cgcccagccg agcgagcgtt
     2641 cgcccgcgat tgctggtgcc gcggcaatgc gctcgtgcgc gaacctgcct tgcgccatat
     2701 ggccgatcca ccggcccacc caggcatagt cgagcgacgc aacgccaaag atgccgcgta
     2761 gaagccgcgc ccacaaatcc attgcgatgg tcgcgccaac tcccacgaat atggtccatc
     2821 ccaattccgc cacatcgcct ccatttgctg cacgccgcgc gttgcgcacg acgattcgcc
     2881 atgatagtgc cacttcaagt ttacttgagg tcaagcgatg aagttgattg atatcgccga
     2941 gctcgcgcgt cgctcaggcg tgccggcctc cgctcttcgc tattacgaag aaaaaggttt
     3001 gatccaatcc gtcggccgtt tcggcctccg gcgaacattt gccccggcaa ccttgcaaca
     3061 gctttcattg atcgctctgg cgcaggcggg gggattcagc ttgcaggaga tcggagaaat
     3121 gtttgcgccg tgtggcacgc cgcgaatcga ccgggcgcaa ctcgaagcga aagccgagga
     3181 gatcgaacgg acgatcagcc gattgacggc gacgcaaacc gcgctgcggc acgtcgctgc
     3241 gtgcccggca tctaatcatt tcgaatgccc cagcttttcg cgcatgctcc ggctcgccgc
     3301 gcgcggacgc gcgcagccgc gcgcgaatcg cacgaaaccg ctacgtcata gaatgggcga
     3361 ttcgcgcaat cgatagccag cgaagcaagc tggacgggtg gaaagacgct cgttgaaaaa
     3421 cggagaccgc atcgacgcct gccgaagcgc gcagtttagc gcaggcgctg gattctgaga
     3481 ggtattcgcc tcagcgataa cgagtgtccg cttctttcag gtctggcctc aaagctgccg
     3541 gtccgccacc ggccattcaa gtcctcaggc ggtatcctac gaccagttcg ttgacgatca
     3601 gcttgggttg cgacggatcg acctcaaact ttggcgcaag tcgcggatcg cgatccgcag
     3661 gtactcgacg cgcttgcggt tcaccagtcc ccgcgcatca cgtagcaccg cagtcgccgg
     3721 ctgatagcca tatgcaccgc tactgtccgg gtcggccccg tgcccgaacc gtcaaacgcg
     3781 ccgcgcggtt gctggcccgc gttagcaccg cattgccgag ccgctcgccc cggacgcgtg
     3841 acgcgtcggt gtcaaagttg atctcgacga accgcggcgt ctcatcgact tcgagcttga
     3901 tcttccagcc gtcacccaag aattgatcgc cgtcgcgaac cattcctgcc tgctccagtt
     3961 gccgactcaa atttggataa gtcgtggcgc taaccttcaa cgagaggccc gttatatcga
     4021 agcggccttg tccatcggcg cggcgcggcg cgaggatttc ggcgcgcgcg atccgctggg
     4081 aagacgagcc gatgcctccc gattgccatt catgaaactc gggacgatag accatgctcc
     4141 acacggccag gtgtggccca ctggcggtat catcgcactg aattgcctca taccatggaa
     4201 ctgggggctg ctgcctgcga tactcgatcg aagtacggcg aaagcgtgat ccgcacgcgt
     4261 ccttccactt tgtggcgagt tcctcgaagt cttttgcatg gtcgtgaccg agtgcgagtc
     4321 ctaccttgcc gacgggttct tcaaacctgt tattgggggc gaagagctcg agataggtgc
     4381 gtctaccgcg aaaaaagatg cggtcggcct ccgggggtgg gagcgcatag tcgggcagcc
     4441 ccgcatcggc ccggccaaac agccgcgcaa gtgtgcggct atcgcgaatc gcgtcgaacg
     4501 tcgactggtc gaggacaatg tagacgtgat tgagcgacgg ggtcgattgc gaagcgactg
     4561 ccgaccctcc acgcgaattt tcatcatctc gggaattcgc gtgaaaggcg gaaagggtag
     4621 ggaacccgcc taccgccagc aaagctaagg caattacgat cttcattgcg tcccttcgtt
     4681 cacctgctgg atgacgatcg cagattatcg cgattgtcgt gcttcactcg gccgcgttaa
     4741 cagccgtcgg acgatgtcag ggcgttgctc tgacatctgt cggtcaccct agacaataga
     4801 tccaaagttt agcaatggca tgatattaca tatcatgatt cggctgctcg aaacgtgggc
     4861 ggcgaaattg gctgatgggg aaatgcatgt ttcggagttt cttgcttgcc ggcgccgcgc
     4921 tcggcgtacc ggcggccgcg ctagcgcagt cggaggctga tgcggagatc gtcgtgctcg
     4981 gcacacgcga acagggatat cgcgcgacgg tcgcgccgca gacgaacaag tccgacacgc
     5041 cgctgaagga aacgccctat tcggtgcagg tcgtcacgcg cgagctcatc cgcgaccgcg
     5101 gaatcaccac gatcggcgaa gcgctgcgct atgtgccggg gttcagtccg caggtcggtt
     5161 tcggggcgag caatgaccgc ttctatgtcc gcggcttcat caccccctat aatctgaaga
     5221 acggccttcg ccgttccgcc tatgcgcccg acgagcagtt gcagaatgtc gaacaggtcg
     5281 aggtgctgaa ggggtcggca tcggcgcttt acggccgctt cgaacccggc ggcgtcgtca
     5341 acttcgtcac caagaagccg ctcgaggagt ccttcgccga agtcagcgcg ctctatggct
     5401 ccttcgatca acttcggttg accggcgatt tctcggtgcc gctgtccgaa acgttgggcc
     5461 ttcgcgtcaa catctcgcac gacgaccgcg acggctttcg cgatttcact ttctcccgcg
     5521 acaccttcat cgcgcctgtt atccaatgga agccatcgga tgcgaccagc gtgaccctcg
     5581 aaggcgaata tgcgcgcaag cgcggctatt tcgatcgcgg tttcgccaat gatccgctgt
     5641 ttctcgaagc gccgcgcgaa cggcaattcg gcgaggaaga tgcgcgctac acgaacaagg
     5701 gcggcgtcgc gagcctcttc gtcgaccacc gcttctccga cgcgctgtcg ggacgcatcg
     5761 ccttcggcta ttcggatttc accaaggaca gctattatta cgccttcggc tttccgccgg
     5821 tcagccgcgc cgatcctgcc gatcccaagg tcaatcgccg cactagtctt gcctatgatc
     5881 ggcagaccga tttgaccgcg caggccgaac tctatgcgcg atttgcgacg ggcgccgtcc
     5941 agcacaaggc gcttttcggc gttgaatatg gctatgatca ctggcggttc gatgtccgca
     6001 acccgccctt cggcgtcaac attccgatcg acttcgataa cccggtctat gggcagcgcg
     6061 ccgagccaag cgtgctcgtc gccgatggcg catggaacag cgattccact gcgatctatg
     6121 cgcaggacga gctgaagctc ggcaattggc gcctccttgt tggcggccgc tacgactgga
     6181 acgaacttcg caatcgcgac tatctggccg gaacgggaga gggggagacg aagcgagggg
     6241 ccttctcgcc gcgtgccggc cttacttgga ccccggtgcc ggcgctctcg ctttacggaa
     6301 gctgggcgcg ctcgttccgc gcgcaggtcg atgttgggcg ccttcgcgac ggttcgctgc
     6361 ccaagccgct catcggcgaa agctgggagg tcggcgccaa ggccgagctt ttcggcggga
     6421 cgctgacgcc gacgctcgcg ctcttcgaca tcacgcgccg caacgtcgcg gtgtccgacc
     6481 ctgtcgactt tgatctcgtg atccagatcg gcaagtcgcg ctcgcgcggg atcgaggtcg
     6541 agattcccgc cgtgctgacg ccgcagtggc ggctccttgc gaactacacc tatctcgacg
     6601 cgaagatcct cgaggacagc tttgcgacgc cgggcgcgcg gctcgtgaac gcaccgcggc
     6661 actcggcgag cctgtggaca acttatgact tcgcgggttc tctcaaaggc gcgagcgccg
     6721 gcttcggaat ccagcatatc ggaagccgcg cgggcaatac cgataattcg atcgtgctgc
     6781 ccgcctacac gcgcgtcgat gcgaacctcg cctatgaatt cgacgccggg ttcgggccgc
     6841 tccgcgcgca gctcaacgtt ctgaacctct tcgacaaatt ctactatgac agcggcggcg
     6901 ccttcatacc gctttatcct ggcgcgccgc gcacggtgac agcaagcctc tcctaccgct
     6961 tcgggggagg ccgatgaggc tgcatctcct cgcgatgggt ttgcttgcga cgctcgcggc
     7021 ctgcgcaccc atcccgatga gcgagccgcc tacgcgtagg gcaatggcgg tcggtgaacg
     7081 gcccgccgcc aacctcgcgg cgggatgcgt tgccgattat cgggcggaca ccgattattt
     7141 tcccgacaag gccgcctttg cgtattcggc ccagctcagg gtcgagtatc acggccactg
     7201 gaagcgcgtg accttcagac cgtcggtcga cacgcgcgag gtgctgcgcc tcgtgctcgt
     7261 ccagtgcggg acgccgccgc cgaagctcgg cccccgcgac acgcttgtcg agatacccgt
     7321 gcgcgatttc gcgaccgcca atgtgtcgct gctcggcgcg tccgaactgc ttggtatcga
     7381 cgaccggctg aagggcgtac cgacgcgcaa cgtctcgatt ccggcgatcg ccgcgcggat
     7441 cgaaagcggc gagacaatcc ccatctatgc cgcgcagcat ggcaatggcg agcaggcggc
     7501 cgcgctcggc gccgacctct tcttcacctt ctattcggcc tatccagacg ccaacatcca
     7561 cccgctgctg aagcggctcg gcgtcgtttc tgcgccgcag gcggatcaca ccgaaccgac
     7621 cccgctcggg cgcgccgaat ggatcaaata tctcgcgctc ttcttcaatc tcgagaagcg
     7681 cgccaatcag ctgtttgacg agcgcgcggc gcgctatgtg gaactgcgcg cgctcacggc
     7741 caatgtcgag gcacggcccc ttgtccagct cggctatccc gagagccgcg acgaatggag
     7801 ccaagccggc ggccgcaatc agctctaccg tatgatcgag gatgccggcg gccgccatgc
     7861 ctggcacgac aatgccattg cggggagcct gacctacgcg ccgatggaga agctgttcga
     7921 tcgcgccgca ggcgcagagg tatggctcgg caatttcatg ccgggggccg cagatatggc
     7981 gacgcttcgc cgcgatcacc cgcggctcac atggcttcgt gcggtgcaag gcgggcgcgt
     8041 ctattggttc gatgccggca aggtcgggcc gttcgcgaac agctggtccg atcagggcat
     8101 gaccgagccg caggacgcac tcgccgaact catcgcgatt ctccaccccg agctgctcaa
     8161 gcctcccgcg aaacagcgct tcatccgcaa gataccggga ggcgcggcat gatcaggccc
     8221 gtttcgttcg ccatcgtgat cggcgtcgtc atcgtggcgc ttttcgttgc cgagctggcg
     8281 ctcggtgtcg tcgacatccc gttcgaggcg atcggcgcaa gtctgactgg cggccaaatt
     8341 gcacaggaag gctggggaca tatcgtccgc gaatatcgcc tcccgcgcgc gctcgccgcg
     8401 ctcgtcggcg gtgcgagcct cggcgcctgc ggcctgatgc tccagacatt gtttcgcaac
     8461 ccgctcgctg accccttcgt gctcggcatc gcgcacggtg cccgcttcgc cgtcgcgctg
     8521 ctcgtcgtcg cgacgggtct tgcgggaccg gcgttcgccg aaagcttcgg cctcgtcggc
     8581 gatgtcggtc tcgcggtcgc ggcctccgtc ggaacgatga ttatcatggg cctgctgacc
     8641 cttctcgcgc gccgcgtcga caatgtcacg ctgctgctct cgggactgat gctcggctac
     8701 atcgcggcgg ggctcataag cctgtcgctt catcttatcg acgaaacgca agcgtcggct
     8761 ttcaagggct gggacgatgc gagcttcgcg ggcgtgacat gggcgcagca catcatcctg
     8821 ctcccggcct gcgcattggg gcttttgctt ctgatggcgc tcgccaagcc gctcaacatc
     8881 ctgatgctcg gtgacacgta tgcgcagagc ctcggcgtac cgttgaaggc gctgcgctat
     8941 gcctgcttcg gcgtcgtcgc gctgctcgtc gggacgatca ccgccttttg cggcgcgatc
     9001 aacttcctgg gcctgatcgc cggacacgcc gcacgcgggg tgctgcagac cgaggatcac
     9061 cgcatcctgc tgccggccgc ggcgctcgct ggcggcgccc ttgcgcttgc gaccgatctc
     9121 atcacccatc tcccatgggc gcggcacctg cttcacctca atgcggtcaa cgggctgatc
     9181 ggcggcccga tcgttctctg ggtcctcctt aggcgtcatg cccggttcgg agccgcggca
     9241 tgagcgcgat cctctccacc gacggcattg cgatcggcta tccggggcgc ctcgttgccg
     9301 accatctctc gcttgacctc catccgggcg atgtggtcgc gctggtcggg cccaacggga
     9361 cgggcaagac gaccttgctg cgcacgcttg cggggctgct cgcgccattg gctggatcga
     9421 tcacgctcga cggagacccg ctcgcctcgc tgtcggcgcg cgagaaggct aaaaagctgg
     9481 gcgtcgtcct caccgagcgg cttcagaccg gctatctcac cggattcgaa ctcgcggcag
     9541 cgggccgcta tccgcataca ggctggcagg ggcggctcgc gcacgaggat atcgcgatca
     9601 tcgacgaggc gcttgcgctg gtgggtgcgg ctgcgtttcg cgaccgcgcc gtcgcaagcc
     9661 tttccgatgg cgagcggcag cgcgttatgg ttgcgcgcgc gctcgcccag cagccgcgtc
     9721 tgctgctcct cgacgaggtc acgtccttcc tcgatgtgcc gcggcgcgtc gccttcttcg
     9781 cgctgctcgg cgagctggcg gggcaaaggg gctatgcgat cgtcatctcg acccacgaac
     9841 tcgagcttgc cctgcgctgt gccaatcggc tgtggctcta cgacggcacg aagctcctcg
     9901 acggcgcccc tgaggatctg atcctgtcgg gcgacttcgc gcgcatattc gccgccgatg
     9961 cggtgagctt cgacgaacat gcgggtcatt tcgagctgcg acgcccagcg aaccggcgcg
    10021 tcgcgctgga cggcgacggg gttcgcggcg catggacgcg ccgtgcgctc gagcgcgcgg
    10081 gatatgcggt cgacgcagct tccgctctcc gcgtttcggt cgacgctgcc ggatggtcgg
    10141 tcggatcgga gcgtgcggac agcatcgccg cgatgctcgg gcggctggat cgttcatgaa
    10201 acgggttggc gttcattcgg ccgctctgct tgtggtggtg cgtgccgcca gcgctgaagg
    10261 cgaaattgtt ctcgacccgt aggcggaaga tagccgctgc attggtcacg gggtggccgc
    10321 tttttagtct ttgcaatctg aaagctgcca gtccgaaatc ggccagcatt ggctagggtg
    10381 ttccatatat ctggtgcgct gcggcttctc ccattcggcg atagtcgtgg ccgacgccgg
    10441 gaatcatacc aagccgccag tttagaagta ggccgagccg cgtggcttcg ctcgtcgcga
    10501 ggtcgtggaa atattctccc cgggcaagac gatgcggtcc ttgcgcgttc gcttccggag
    10561 acgaacgcaa atgcccgcgc gtttcgtggg cgtcgtctcg ttcgcccaac aacaggacga
    10621 gcctacgccc aaaggccttt tcgagctgct ccccgggcag cgaaatgccg ccgagcccat
    10681 aagggaacga aacattctgc gcagggaccg tataccaccc tgaattggct gcaaccgccg
    10741 tccttaccct cgtttccggc gcgaagagaa tcatgcgatg aacgatctgg gcccccgccg
    10801 agtggccgaa caggtcgtag cgtgtctgtc gggaacccgt tcgcgcgaca gcttgatcga
    10861 agatcgcctc catatcggag aagagccaag tttccggctg ctccaatacg acgttgcgaa
    10921 gggtggccac gtccgcatct tcgcgaacca tgcccgccag attatagttt atcggtcccg
    10981 ggaactgcgc ctcatcgaaa gatggcgcca atatgagcag acgaaagcgt tccgccgccc
    11041 ggatccagct gtcccgatag tcggcgccgt tccggccgcc cccggctaac aatatgatga
    11101 ttggatcgtc cggcccatga ttttcagcgc gataggtaaa gacctttatg gtctcgccgc
    11161 cgggaagggc cgcctcgaat gaactcgcgc caacggcgat ctcgcttatg ggacgcgcgg
    11221 ctgcggagag cgttggcggg ctaccggccg atggagcggg ggataccgtc aggccagcca
    11281 gaaagaagaa ggggagaaga tgatacttcc agctttcttt gataatcata aagtcctcac
    11341 ggctgcttgc cgacccgcgg catgcagtaa gtgttccggg cgcaacccga aaggaaggct
    11401 gggtttcgtg tgatgtccga atttgtcgta aacggtccgc cgggcagccg gcgagacatg
    11461 acgctccatc ttggagggat cgcggcgctc accttggttc tggtcacaag cggcgcgttc
    11521 gatagcgacg atctgccgct cgcgcgccgt ctgctcatgt tcggcgtcat ctcggggctg
    11581 ctggtggtgc aggcctcagt gatcgcggac gtggcacggc gatattctgg atcttcgctg
    11641 atcgaaacgg ccacggccgc agtcttctcg ctcgtgacga cactcctgct gatgaccatc
    11701 gaagtccatt tgctgaaagc gactgtggtc gttccctatg aaccggaccc attgcccgac
    11761 tttgcgcttt ttcttgcgcc gttcgtcata ccggtgggcg cgctggtgct ttgcataaag
    11821 tggtttggag tcgccagacg cgattcccct tcggtgtcct cggttgggtc tctcaccccg
    11881 ccggagccat gcgacacgtc gccaccaatg cccgcgttga tcgaaaactg gccccgcgag
    11941 ccaattcttc gtatcagggc tgcggaccat tatcttgaga tttggacggc gggcggcatt
    12001 tcgctcgtcc gtggccggat gcaggatgcc ctgcgaaggg tatctcgaac ggccggcatt
    12061 cagcctcacc gctcatggtg ggtgtcgatc acggaaatag accgaatcga gcggcgcggg
    12121 cgcgattggt tcctcatgat gaaagacgga gaaagccttc ccgtggcgcg cggcagggcg
    12181 gctgcagtca ggctttctct tgaagaccaa cggacagact gacaatccga ttggaaacgg
    12241 gcggcatgca ccccggaatc gcagcggtta ggggaatgct cgatcgaaaa gcagctcacc
    12301 gccgcccgag acgaccaggc tttcgacccg gccgaaggcg ttgcgcttga acgccagctg
    12361 aagcgtaccg gcgggaacat aggcatccgg agcgacgaag cgcgcaccgc ctatcggagc
    12421 gagccgccgc tcacgtccgt ccgccttcag gctatccggc acgacttgtt cgcctgggaa
    12481 agattgctcc gaatttgtcg atttggcgca gctcgctccg aaaggtcagc aattctcgct
    12541 agttcgtcgt tggtataagg ttgcgcgcct tgacgaaaag gattggaaat atggaagtct
    12601 ttcttgctgt ggccgtcgtt tgaccgaata tgccgaactc gctgttctgg agctttatga
    12661 aaggggtggc tgccgatccg ggcggcaaac tattttggaa ctgacgccga cgggaccggt
    12721 aaaccgaggg gtcatctact tgaatgtcca gcgtggtcag aaaaacggcc acaccgagag
    12781 ccagtctggt cgcatagaaa atgtcgtgat cggtaagtcg tttgatgttg tcgtccccca
    12841 caccgatgtt cgcgagagat atgaagtcag ttcgggacga tattttggcc cggccaagag
    12901 caggctgcga tgctgaccgg cggacattca tggctaattg ggcaaatcac ctgcacgtct
    12961 cttttttatg atcgtgagct atatgcggac cgtccggaag cggccaggac tagcggttca
    13021 tgttctgtcg acagattatg ccggcgaaac ctcgccggtg ggatgcaagg cggatgacgt
    13081 agaggtcctg aattccccct cccgatcggg ggagggcagg tcagaccgca gcgatctgtg
    13141 cttcgtcatt ggcggcgacc tgccgtgcga tccgcacggt ccgggtcagc gacacgcggg
    13201 ttcccggcgc ggtgtcctcg accgcaatct ccgcccccaa ctgcttggcg agtgcctgga
    13261 cgatgcgtgt gcccagtccg gcagtcgatc gttcggtatc ctcgtccatg ccgaccccgt
    13321 cgtcggacac cgagagcgtc cagtcgtcgc cctgtgcgac gtagccgacg acgatatggc
    13381 cggggcggcc gtccggaaag gcgtgcttga gcgcgttgat caccagttcg gtgacgacca
    13441 gccccatgct gaccgacaca tcgccgctga tcgataccgg atcgacgtcg gtggtaagcg
    13501 tcaattgctc gcggtcatag atcatcgacg cgccgatgct ggcgcacaat tgcgcgagat
    13561 agggttcgag ctcgaccgtc tcgccatcgg atccggcgag gtgacgctgc agcgtcgcga
    13621 tcgacatcac gcgttgatgc gcgtcgctca aatggccgcg cgcttcgtcc gactgcacct
    13681 tgcgcgcgct ctgcatcaat atgctcgcaa taatctgcag gctgttggcg acgcggtgct
    13741 gcatctcgtg aagcagcgcg gcattggcgt gaaccaggtc cttttggcgc ttctcgttct
    13801 gccgctcctc ggtgacgtcg atcacggtga ccatcatccg ggtgttcagg ccgtcgccat
    13861 aatcgagccg ctgcgccttg atcacgacgc atcgcaccgc ccccgcgctg gtcttgatct
    13921 ccatctcata ggcttcgacc tcggcggcgc ccgacagcgt cgcttcgatc agcgagtgca
    13981 gccgcggcaa gtcccaatga tgatggtcga ggtcatagat cgtctgcccg gtgacggttt
    14041 cgggatcgat accgaaggcg gcacagaaac tgtcgctcgc cgcgacgacc gccagatttt
    14101 cgtcgagcag aagcagcggc gcatcggacg cgagcaccag cgctagccct aatgtgcggc
    14161 ccagattgat aggtggattg tcggcagcca tgcggcgcgc cttcccggtc gtcccggacc
    14221 gcagggcgcg gccttgtcgg cagaccaaat cgcggagcga gttgatcgac gtcgtcgatc
    14281 attgccccaa ggctgcgggc tttagcatgc cggcgggagg agggatgcat ggaatcgaca
    14341 cgacgtaagt gggacggaag gcgacagaca tcatcggttg attgatgtcg cgaccctggc
    14401 gacgtgccca aatgccggaa atccagaatc tacgcttaaa aggaagctga acggcggccg
    14461 ggaccagcga ccgccgtcct actttatgcc gcatgtgcgt ggggcacagg gggtgcggca
    14521 cggtctcatc gtggggccag atggataaca aatccttaat ttcaggcaat ttgcggcgag
    14581 atgagtcggg cggagtttga aagcgagccc gatccccagc tgatctgatc cgcatccgat
    14641 ccttgagtgg cgctctggcc gcgtctcctg agagccatga gacaagtggc atcaggagac
    14701 ggggttgtcc gaaaaacgcg ggaacgccgg ttgaagttcc ggcaaagcca ttatgcggca
    14761 gcagatggag cgataatgtc gtcggtgatg ctgcgcgcaa atatggagca cggcaaaaag
    14821 accccgcgga gggcggggcc ttttcgttcc cctgaatgat ccaggagaag ccccatccgc
    14881 ctcgggggtc gcaacccgaa ggcttcagga aagcggttaa atcaatcgtg cggtcgaagc
    14941 cgtcggccca tcagtcgggt ttctcgatca atagttttac gctggaaaag tctctcacag
    15001 gggaaaggat aacagcctga accggggcag attggcccgc ctgctatttg acggcctctt
    15061 gggccgcgtc gcccacatcc tgagcggcat cgcctacctt ctgcgctgcc tcggtgaccg
    15121 caccggtttc ggccttctcg ttgccgagga actggaatcc tagaaatgcc gcgatagcca
    15181 gcgcagcaac gacgaggagc gtgatgcccg ccccgccacc gcgtttcgga tcgcggtcga
    15241 tgactgtcgt ggtatgtgtc gttccgtcgg gttcgcgggt tgtatggatc tcatcagcca
    15301 ttggaacctc ctctgaagga tatcgcttag gacgtttcct tcggacgaag gttccggtcc
    15361 cggccagtag acagcacggc cgcgcccgcg atgctccaag acgagtgttc gctctcgcac
    15421 atttttgcgc cgcaaccggc ccgaccgctt tcggccagac ctctggtgca ccaaggagag
    15481 gcatgaggac ctcacctttt ctcccaggtt caggaatctc cgcaggtcgc aagcaatttc
    15541 ttagcgcaac gagcggctaa acgcttcgtt aaatggagga gcgatatcct cgctgaccac
    15601 tatttgcggg attgcagtaa aatggccctc ggcgcgagcg ctaatttgtc cgttcaatcg
    15661 gaagctgtcc tccgcgagca gtatgcgagc ctggtgcggc aggttccgct catgtacgcg
    15721 ctcatgtttc tcaacgtgtt gtttcttgcg ctcgtgaccg cggacgggga atcgtgggcg
    15781 attagcttcg tggcaccgct cgccttgacg ctgatcatcg cagttcgcgg ggcgacgtgg
    15841 tatgcgcgca ggggcgccct cgcgaccgac gatcatatac gcttatatct acgcggcacg
    15901 gtctttcggg ccggtgcctt cagtctcttg ttcgggggct ggggtctcta tcttttcctg
    15961 gctgccgacc cgtttcacac aaccgctatc gcgctattca tattcgtagg ttcgatcagc
    16021 tgttgctact gcctgcaggc actcccagcc gcggcgcggc ttgttctcct gttcggtgcg
    16081 atgccggtca ccctcgggct ccttgtctcg cgtgattggt acttcaccgg gatgggcctc
    16141 acgttcctgc tcggggcggg cgtcatcgtg cgaacgatcg cgacgaccca ttcagcagta
    16201 ttcgaatcgc ttcgttcgcg ctcggaaatg tttgcgctga tcgaagccct caggcgtagc
    16261 gaggaacatt atcggttttc agtcgagctc aacccgcaga ttccgtggat cagcgatccc
    16321 gaaggccaga tcgtggaagt gggaccccgc tggaccgagc tcaccggcgt gcccccagag
    16381 gacgcgcttg gccgaggctg gaccgagtct ctccatcccg acgatctgcc cgttgttctc
    16441 gcgcactgga acgacgcact gacgtcggtc gacaatgccg ttgccgacgt acggtaccga
    16501 gttcgcaata tcgacggcag ttacagatgg tgccgcgcgc gagccaatcc gcgtcgcgac
    16561 gccgacgggc ggatcgtcag ctggtatgga accctggaag atatccacga tcaggtgatg
    16621 gcggaactgg ccttgcggga gagcgaggag cgttaccgcc tggcttccca cgccacgaac
    16681 gacgtgatct gggactggtc tcaccgcacc aatcgcatcg aatggggcaa tggcgcggta
    16741 gatgtgctcg gttatccgga agcgtcgaac ggcacggcgg tgagctggtg gaaagagcgg
    16801 gttcatcccg atgacctgcc cggcgtggaa gcgacatatg acaaagtgct acgcaatctc
    16861 caggacagct ggagtcacga atatcggttc cgcgccgcac agggcaattt catcaacctc
    16921 ttctcgcgag gctatgttgt gcgcgacgac gaagggcatg ccgtgcgttc gatcggcgcc
    16981 cttcttgacg tcaccgccgc gcgccgcgcg gaagaggaac tgcgctgggc cgcgaaccac
    17041 gatccgctga ccggtctgcc caaccgcaag ctcttcgccg aagtgctgga gcaggccctg
    17101 acggccgcgg ccgccaacgc gtcttatgtt tgcgtcatcg tcatcgacgt cgatggcttc
    17161 aagctcctca acgacagcat ggggcatgcg gcgggcgatg agcttcttag aacaatagcg
    17221 cggggcctgc aggggaacct tcctgccgag gcaacggtcg cccggcttgg cggcgatgaa
    17281 tttgcggtaa tcgtgccggg actggcgcct gattcacctc gcgcttcggc tgttgatgca
    17341 gtcctcaagg gcatagccga tacgctgacc attgatgaga gcgtcttgcc ggtcagcatc
    17401 agcgcgggcg ccgcggtgtg gcccaccgac ggcgatgacg ctgaagatat attgaagagt
    17461 gcagatcttg cgctttacgc ggcgaaggca gagggggcgg gggtcgttcg cgaattccgc
    17521 cctgaaatgc gcgaatccgc agaaagccgc aagtcgatgc tgcgcgaggc gcgcgaggct
    17581 cttcgtgatg atcgggtcat ccctttctat caggcgaagg tcgaccttcg cagcggcgat
    17641 gtgatcggat tcgaagcttt gctgcgctgg catcatcatc ggcgcggtct tcagccgccc
    17701 gacagcatcc aggccgcatt cgacgacagc ctgctggcga gcgaactgac cgaccggatg
    17761 atagaccgcg tgctcgccga cgttggggag tttaacgaga agggcgttgc ctttggccgc
    17821 atcgcgatca acggctcgct tgcggatttc cggcgcgatg acttcgccga tcgcattctg
    17881 ggaaagtttc agcgggcggg acttccgccg acgctgctgg agctcgaagt caccgaaagc
    17941 gtgttcgtcg atcatctggc atttaacgta gagcgtgccc tgcggacgtt ggaggccgag
    18001 ggcgtttcga ttgcgctcga cgacttcggc acaggctatg cttcgttgac gcacctccag
    18061 caatttcctg tccacgtgct caagatcgac cgatcgttca tctcgcgtct cgacagcacc
    18121 gacagcgccg atttcgcaat cgtgcatggc gtgatcgaca tcgcgcatcg gatgaacatc
    18181 gtcacggttg ccgagggcgt cgaaagtgat aatcagctcg ttcggctgcg cagcctcggc
    18241 tgcgacatcg cgcaaggcta tcttttcagt cgcgcaatca gcgcagaacg cgtaccggct
    18301 tggctgagaa aatgggcaca ggaccctcca ctctggttgg aggccgcgac cgaccgggcc
    18361 acagccgacc cgccgacccg ctctgcatga caaggacggg actcaccgcg ctgctattct
    18421 tgtgccggct ttccgttcct taatagcagc cttcttgctg ctcgtcgcta tctgaacctt
    18481 ggcggcgtcg acctttgcgc cgctacgccg gctaaagtcc cgggtcctgc cgcgagcgct
    18541 caaatgccag ccgagactgc atttcgcccg gcaatgtcgc ccgcgtgggc cgtcgggctt
    18601 gccggcgcgc tctgactcag ttcagatgcg gaccgccttt cggggcacaa aggtcggctg
    18661 gatattctcg gcagcccacc ggttcgcgaa atcgactccg atttggttcc ctcgagccgc
    18721 aacgaacggg acgtcgcgca ggttgcgcac atgggttacg gccgcgcgct gccgttgttc
    18781 aggtcggccg gcttgccccg attgccggac gcaactactc gggatattgg gccgcgcaga
    18841 ggcgctggca atcgtcgatg agttcggaca tcgccgcagg cttcgcataa aaggcgctgc
    18901 cggcgacctg gcgatggggg agcggcgcat tcgccgacgc atagatgacc ccgccttgcg
    18961 gccgaagcga ccgataggcc tcggcaaggt cccacccggt catatcgccc gcgaggcgta
    19021 tgtcggtgac gaggacatca atggcaccgc cgttgccgag cgttcgcagc gcatcttcgc
    19081 ccgcgtcggt ttgtttcacg tcgaagcctg cggcttcgag cgcatcgacg agatccatgg
    19141 cgagcagcca ttcgtcttcg acgacgagaa cggaacagcg atgcgggggg atggcggtcg
    19201 tcacggcaag ctcttcatca atcatcgcac ctggcgccat gcgcacggtt agcggctcgg
    19261 cggcgtgaaa ggaacggtct tgcgggccga gagtttccca gagcgggaat aaatgcgcgg
    19321 gcgcatatat ccgatctagg aattttgccg gatggcggac ggaaatgaac gcgcggcgag
    19381 gcccgacagc gaaatcgcta cccatcacct ggccgcgatc gtcgagtcat cggaagacgc
    19441 gatcatcagc aaggacctta acggcatcgt cacgagctgg aacaaggcgg ccgagcgatt
    19501 gttcggcttt tcgccggcgg aaatggtggg acaatcgatc ctgacgctca tccccgatga
    19561 gcgccgcgac gaggaagacc tcattctcgg gcgcattcgc agcggcgacc gcgtcgacca
    19621 tttcgagacc gttcgccagc gcaaggatgg ctcgctcgtc gacatttcgc tcaccgtctc
    19681 accgatccgc cgcgccgacg gcacagtgat cggtgcctcg aaaatcgcgc gcgatatcag
    19741 cgagcgccgg tcgaccgaag caaagcttgc cgagcaggcc cgcacgctta gccagctcaa
    19801 ccgggtcgcg acgattgtgt cgcaggatct cgatcttgag cggatcgtgc agtcggttac
    19861 cgatatcgcc accgaactca gcggagcgcg cttcggggcg tttttctata atgtcctcga
    19921 cgaaacggga gaaagctatc tgctcttcag cctctcgggc gcgccgcgtg atgccttcga
    19981 gcggttcgga atgccgcgta atacagcggt gttcgatccg acctttcgcg gcgagggcgt
    20041 cctacgctcg gacgatatcc ggctggacgc gcgctacggg cacaacgacc cgcatcatgg
    20101 catgcccgag ggacatcttc cggtcgtgag ctatctcgct gtcccggtcg tgtcctcgtc
    20161 gggcaaggtt cacggtggcc tctttttcgg gcacgacgag ccgggcaaat tcgacgaggg
    20221 catcgaggag ctgatcgtcg gcattgctgg ccaggccgcc gtcgcgatgg acaatgccca
    20281 tctgcacgaa gccgcacggt tcgagatcgc gcagcgcgcc cgaaccgaag aggcgctcga
    20341 gcttctgctc ggagagcttc ggcatcgcgt caaaaatacg ctggcgatgg ttcaggctat
    20401 tgcgtcgcag acctttctcg ctgctcccgc tgaagagcgc cgctcgttcg aagcgcgcgt
    20461 gcgcgccttg tccgacgcgc atgatctcct caccgagaag aattggggga ttgtgtccgt
    20521 tggcgaaatg atcgagcgcg cacttcatgt gttcgtgaaa gtccatcccg gacgcatcgc
    20581 gatcgacggg cccgatgcat cttttcccgc cagcaaggca ctgctcgttg cgatggcgct
    20641 tcacgagctc ggaaccaacg ccgtcaaata tggcgccttg tcgaatacgg cggggacagt
    20701 cgacgtgagc tgggaactga tcaccgaggg tgcgcatcaa ttcctgattt tgacatgggt
    20761 cgaggcgggc ggtccgccgg tcacaccgcc cacgcgacac ggttttgggt cccgaatggt
    20821 cgagcgtgcg cttcgcagcc agcggggcaa tgccgagatc gaatttcgcc cggagggcgt
    20881 acgctgcgtc ctgcgcctgc cggcgtgacg cgattgggga tgctgggtcc agcgaagctc
    20941 ttttctagtc ggcattgttg ggatcgcgcg ccttctcggc ttcttcctct tcggcgcggc
    21001 gcattctagt cgcctcttcg gtgcgccgtt tctcggccgc cgaccggcga ttgttgagca
    21061 tcgcccaagc gagaaccagg acaagcaaga tcggtccgat gattgtcata atgccccatt
    21121 ccatcggttc gctccttcca tgcgcgtttg cgattattcc agaacaaacg gcgggcgcct
    21181 tggctgcatt tttaggctga gccgatattt tcgcttacgg cattccgtgg tagggctgtc
    21241 gttgggcgac cccggctatt ctgcgatgcc ggggcttgcc tgggaaacat cgtgaccagg
    21301 attccggaca atatatgggc cgcgatgacc gcggccgagg acgcatacgg cgagggcgcg
    21361 gaggcctttg cccgcggaaa ggcggtcgag gccgaagccg ggggcaacac cgaacaagcc
    21421 agcgagtggc accttgtcgc ggatgaactc cacatcctgc atgcgatcaa tcgccaatgg
    21481 gtccggccac gaagcacgtc gcggccatcg acttgagtgc atgcgggcgc gtccacggag
    21541 aatcccggcc gccttgccta tttgaaacaa ggccagaccg acccagcttc gcagattagc
    21601 gacatctagc gggcgtaagc gacggcttgc gatttctcaa atggcggatg agcaatcgcc
    21661 gcgatcgcat cggaccggaa gccgggaagg gcgcccaggt gaccaagcag caaatcccat
    21721 ccgggcaacg cctcgtgaaa catccaggtt tttccggcga ctttgacgtc gcagcagatc
    21781 aggtccgccg tgacttcgtc acgcttgtag aacacgatcc gctcgatctc tcccagcgga
    21841 agatgaaaaa ggagctcggc gtcgatatca gtcatgcgtc accacaaaat cgccccgccg
    21901 gagcggggca gtttgagggt tccaaggggc ccgctgccag gaggggcggg gtgttatagt
    21961 aacgcacgct gtccctctgg gttccaacca gatggcggga agatcgccgc cgttgcatcc
    22021 gcgtctcgaa acaaatgccg cttatcaagg ggctgacgcc tcttgactcg actcgcttcg
    22081 ctcccaaaat ctgtctctgc ggcaccttgc cgcacgacga gaaaggaaag gctacaagga
    22141 aggaggcacg atcatgtccg agccattttc ccgttttc
//
LOCUS       DATMSU010000527         1960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_141433,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000527 DATMSU010000000
VERSION     DATMSU010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141433"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="ssb"
                     /locus_tag="VN034_14150"
     CDS             complement(<1..543)
                     /gene="ssb"
                     /locus_tag="VN034_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWV61801.1"
                     /translation="MAGSVNKVILIGNLGADPEIKSFQNGGKVANLRIATSEQWKDRM
                     TGERKERTEWHQVVINGEGLIGVVERYLKKGSKVYIEGSLRTRKWQDRDGNDKYTTEI
                     VIAGMGGTLVGPLIDRAAFDGMQKALNESREIGATVFALIRFIPGDPAALMLGDMAQP
                     EQIEAMRTELGLDKSMPQQFV"
     gene            complement(576..>1960)
                     /locus_tag="VN034_14155"
     CDS             complement(576..>1960)
                     /locus_tag="VN034_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HWV61802.1"
                     /translation="AELLAAVDGAIRSHSAGEVAVPAKANDASLHQRARTLARAAILS
                     ETPVRRWTQRVDNVVLHPVAGMIILLALMFVMFQAVYAAAEAPADFLEGGIGVVQQWV
                     VATFPDNFLRGLVVEGLLAGVGAVVVFLPQILILFLFILLLEASGYMTRAAFLMDGLM
                     GKVGLSGRGFIPLLSSFACAVPGIMATRAIPDEKDRLTTILIAPLMTCSARLPVYALI
                     IAAFIPNTNVGGSPVGLQGLVLFGLYLSGIVGALVVAYVLRRTVAKGAGAGFMMEMPR
                     YQMPRWRDIAIGLWQRAWIFLRRAGTIIAMTTVILWLLLTFPQAPEGESQVEYSVAGR
                     IASGLEVVVKPIGFNHDIALALIPAMAAREVAVSAMATANAIDASGDEEAMAQSLGER
                     LQGKWSLATALAFLAWFVFAPQCISTIAITRRETNGWKWPMFMVGYLFLLAYAAAGIT
                     YWTAVAFGLG"
BASE COUNT          364 a          637 c          624 g          335 t
ORIGIN      
        1 gacgaattgt tgcggcatgg acttgtcgag gccgagttcg gtcctcatcg cctctatctg
       61 ttccggttgt gccatatcgc ccagcatcag ggcggccgga tcgcccggaa tgaagcggat
      121 gagcgcaaag accgtggcgc cgatctcgcg gctttcgttc agggccttct gcatgccgtc
      181 gaaggccgcg cggtcgatca gcggaccgac cagcgtgccg cccatgccgg cgatcacgat
      241 ctcggtcgta tatttgtcat tgccgtcgcg gtcctgccat ttgcgcgtgc gcagcgagcc
      301 ctcgatgtag accttcgagc cttttttcag ataacgctcg acgacgccga tcagcccttc
      361 gccgttgatc acgacctggt gccactcggt gcgctccttg cgctcgccgg tcatccggtc
      421 cttccactgc tcggacgtcg cgatgcggag attcgcgacc ttgccgccgt tctggaacga
      481 cttgatttcg ggatcggcgc cgaggttgcc gatcaaaatt accttgttga cgctgccagc
      541 cattgccgct ccgctccgct agaaaatgtg gaggatcagc ctagtccaaa ggcgacggct
      601 gtccagtaag tgattccggc agcggcatag gcgagcagga acaaatagcc aaccatgaac
      661 atgggccatt tccacccatt ggtctcgcgc cgggtgatcg cgatcgtcga aatgcactgc
      721 ggcgcaaaga cgaaccaggc gaggaaggcg agtgcggtcg cgaggctcca cttgccctgc
      781 aatcgttcgc ccagcgactg cgccatcgcc tcttcgtcgc cgctggcgtc gatcgcgttg
      841 gcggtcgcca tcgccgacac cgcgacctcg cgcgccgcca ttgcggggat cagcgcgagc
      901 gcgatatcat ggttgaagcc gatcggcttg acgacgacct cgagcccgct cgcgatgcgc
      961 ccggcgacac tatattcgac ctggctttcg ccctcgggtg cctgcgggaa ggtaagcaac
     1021 agccacagta tgacggtggt catcgcgatg atcgtgcccg cgcggcggag gaatatccac
     1081 gcgcgctgcc acaggccgat cgcgatgtcg cgccagcgcg gcatctgata tcgcggcatt
     1141 tccatcataa agccggcgcc cgcacccttt gccaccgtgc ggcgcaggac ataggcgacg
     1201 acgagcgcgc cgacgatccc cgacagatag agaccgaaga gcaccagccc ctgcagcccc
     1261 accggcgatc cgccgacatt ggtgttgggg atgaaggcgg cgatgatcag cgcatagacg
     1321 ggaagccgcg ccgaacaggt catcagcggc gcgatcaata tcgtcgtcag ccggtccttt
     1381 tcgtcgggaa tcgcgcgcgt cgccatgatg ccggggaccg cgcaggcgaa actcgacagc
     1441 agcgggatga aaccgcgccc cgacaatccg actttgccca tcagcccgtc catcaggaag
     1501 gcggcgcgcg tcatatagcc cgatgcttcc aaaagcagga tgaagaggaa gaggatcagg
     1561 atctgcggca ggaagacgac gaccgcgccg acgccggcga gcaggccttc gacgaccagc
     1621 ccgcgcagga aattgtcggg gaaggtcgcg acgacccatt gctggaccac cccgatcccg
     1681 ccttcgagga agtccgcagg tgcctcggcg gcggcataga ccgcctggaa catcacgaac
     1741 atcagcgcga gcaggatgat catcccggcg accggatgca gcacgacatt gtcgacgcgc
     1801 tgtgtccagc ggcgcactgg cgtttcgctg agaatcgcgg cgcgcgcgag tgtgcgcgcg
     1861 cgctggtgga gcgacgcgtc attggctttg gcgggcacgg cgacttcgcc cgcgctgtgc
     1921 gaccggatcg cgccatcgac ggcggcgagc agttcggcca
//
LOCUS       DATMSU010000528         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_141438,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000528 DATMSU010000000
VERSION     DATMSU010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141438"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..546)
                     /locus_tag="VN034_14160"
     CDS             complement(64..546)
                     /locus_tag="VN034_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWV61803.1"
                     /translation="MTIQTGDKLPDATFVKVTENGPEQVNTADYFKGRKVALFSVPGA
                     FTPTCSAKHLPGFVDKADELKAKGVDEIVCTAVNDAFVMGAWGKSGNAGDSVTMLADG
                     NGAFAEAVGLTMDGTAFGMGKRGQRFSMIVNDGVVEQLNVEAPGEFKVSSADHMLEQL
                     "
     gene            complement(707..>2846)
                     /locus_tag="VN034_14165"
     CDS             complement(707..>2846)
                     /locus_tag="VN034_14165"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61804.1"
                     /translation="VTAQRREESLQDVPVAVSVLSGDTLGAITSTGADVRVLAGRVPS
                     LNIESSFGRTFPRFYIRGLGNTDFDLNASQPVSLVYDDVVLENPILKGFPIFDLDRVE
                     VLRGPQGTLFGRNTPAGIVKFDTVKPGKTGGYARVSYGRYGTSQVEVAAGAADDSGFS
                     VRLSGLYQHRDDWVDNIATTKKDDLGGYDDIAARLQLQYESGPFTGRVTGQVRVYDGS
                     AIIFRANTQVPGSNKLVGLGGAGTEFERDKVWQDGINFQKLNTYNVGLNLEYDFGPVT
                     AYSITSYWNGNFRSRGDIDGGFGAVFLPESGPGLIPFQAQSQDNVPSLDQFTQEIRIA
                     SNNSGGLGYQFGAFYFDEKLDISSFEFGGPTDATPSAIAIQRQDSEAYGIFGSVNYEF
                     EGGFKLQAGARYNNDTRDFVASRPVETRPPFVVSPNTPVPPQAARVKGKLLTWDASAS
                     YEASDALTLYARVARGYRAPSVQGRLTFARTISTADQEETMSYEAGIKTAFLDDRVRF
                     NLTGYFFDTKDLQLTAVGGTANVASLLNVDAKGHGLEAELQAAPARGLTFSVGGAWNV
                     AEIDDANAFVAGCGSATPCTVLDPQRPGSPNIFSIDGNQLPQSPKWTLNWTAGYEIPV
                     GDGAIYAFTDWYYRSKVQFFLYRSVEFSDDKMIEGGLRVGYKTDRFDIAGFVRNITND
                     ESPTGGIDFNNLTSYVNEPRIWGVEAGVKF"
BASE COUNT          471 a          868 c          988 g          519 t
ORIGIN      
        1 gccatgccgg aggcgatccg gcatgaccgg gcgggacggc gcggcatcgc gcccggtgcc
       61 gccttaaagc tgttcgagca tatggtcggc gctcgacacc ttgaattcgc cgggggcttc
      121 gacgttgagt tgttcgacga cgccgtcgtt gacgatcatc gagaaacgct ggccgcgctt
      181 gcccatgccg aaggcggtac cgtccatggt gaggcccacg gcttcggcaa aggcgccgtt
      241 gccgtcggcg agcatggtga cgctgtcgcc ggcattgccg ctcttgcccc acgcgcccat
      301 cacgaaggcg tcgttgaccg cggtgcagac gatttcatcg acgcccttgg ctttcagctc
      361 gtcggccttg tcgacgaaac ccggcagatg cttggccgag caggtcgggg tgaaagcccc
      421 cgggaccgag aagagcgcga ccttgcggcc cttgaaataa tcggcggtat tgacctgttc
      481 ggggccgttt tcggtgacct ttacgaaggt cgcgtcgggc agcttgtctc cggtctggat
      541 cgtcatgtcg gcatcctttc atcgagggga tttgggtcgg acgcgacatc gggtgcggca
      601 gcgcgcgagt caaggagggg aagggcacgg cggtcgattt ggaggatgac ggccgctccc
      661 cgcccagggc accctccccg ccgcagcgac ggggagggcg gccggatcag aacttcacgc
      721 ccgcctctac gccccagatg cggggttcgt tgacgtagct cgtcagattg ttgaaatcga
      781 tgccgccggt cggcgattca tcattggtga tgttgcgcac gaagccggcg atgtcgaagc
      841 ggtcggtctt gtagccgacg cgcagcccgc cctcgatcat cttgtcgtcc gaaaattcga
      901 ccgagcgata gaggaagaac tggaccttcg agcggtaata ccagtcggtg aaggcataga
      961 tcgcgccgtc gccgacaggg atttcataac ccgcggtcca attcagcgtc cacttcggcg
     1021 actgcggcag ctggttgccg tcgatcgaga agatgttggg gctgccggga cgctgcggat
     1081 cgagcacggt gcacggcgtc gccgaaccgc agcccgcgac gaaggcgttg gcgtcgtcga
     1141 tctcggcgac gttccacgcg ccgccgaccg agaaggtcag tccgcgcgcc ggtgccgcct
     1201 gcagctcggc ttcgaggccg tggcccttgg catcgacgtt gagcaggctc gcgacgttcg
     1261 cggtgccgcc cactgccgtg agttgaagat ccttggtgtc gaagaaatag ccggtcaggt
     1321 tgaagcggac gcggtcgtcg aggaaggcgg tcttgatccc cgcctcgtac gacatcgttt
     1381 cttcctgatc ggcggtcgag atcgtccgcg cgaaggtcag gcgcccctgc accgacggcg
     1441 cgcgatagcc gcgcgcgacg cgcgcgtaga gcgtcagcgc gtccgaagct tcatagctcg
     1501 cgctcgcgtc ccacgtcagc agcttgccct tgacgcgggc ggcctgcggc gggaccgggg
     1561 tgttcgggct gacaacgaag ggcgggcgcg tctcgaccgg acgcgacgcg acgaaatcgc
     1621 gcgtgtcgtt gttgtagcgc gcaccggcct gcagcttgaa gccaccctcg aattcataat
     1681 tgaccgaacc gaagatgcca taggcttcgc tgtcttgacg ctggatcgcg atcgccgacg
     1741 gggtcgcgtc ggtcgggccg ccgaactcga agctcgaaat gtcgagtttt tcgtcgaaat
     1801 agaaggcgcc gaactgatag ccgagcccgc cgctgttgtt cgacgcgatg cggatttcct
     1861 gggtgaactg gtcgaggctc gggacattgt cctggctctg cgcctggaag ggaatgaggc
     1921 ccggacccga ttcgggcagg aacacggcgc cgaagccgcc gtcgatgtcg ccgcggctgc
     1981 ggaaattgcc gttccagtag gaggtgatcg agtaagcggt gaccggcccg aaatcatatt
     2041 cgaggttcag tccgacatta taggtgttga gcttctggaa gttgatcccg tcctgccaca
     2101 ccttgtcgcg ttcgaattcg gtgccggcgc cgccgagccc gacgagcttg ttgctgcccg
     2161 gaacctgcgt gttggcgcgg aagatgatcg ccgagccgtc atagacgcgg acctgtccgg
     2221 tcacgcggcc agtgaaggga ccgctttcat attggagctg gaggcgcgcg gcgatgtcgt
     2281 catagccgcc gagatcgtcc ttcttcgtcg tcgcgatatt gtcgacccag tcgtcgcgat
     2341 gctgatagag gcccgagagg cgcaccgaga agccgctgtc gtcggcggcg cccgccgcga
     2401 cctcgacctg gctcgtgcca tagcggccat agctgacgcg cgcatagccc ccggtcttgc
     2461 cgggcttgac ggtgtcgaac ttgacgatgc ccgccggcgt gttgcggccg aacagggtgc
     2521 cctgcggccc gcgcagtacc tcgacgcggt cgaggtcgaa gattgggaag cccttgagga
     2581 tcgggttttc gagcaccaca tcgtcgtaaa cgaggctgac cggctgcgac gcgttgaggt
     2641 cgaagtcggt gttgccgagg ccgcggatat agaagcgcgg gaaggtgcgg ccgaacgagc
     2701 tttcgatgtt gaggctgggg acgcgacccg cgagcacgcg gacgtcggcg ccggtcgagg
     2761 tgatcgcgcc gagcgtgtcg cccgacagca ccgagacggc gacgggcacg tcctgcaggc
     2821 tttcctcgcg gcgctgcgcg gtgacg
//
LOCUS       DATMSU010000529        10180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_75372, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000529 DATMSU010000000
VERSION     DATMSU010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75372"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..2914
                     /gene="secA"
                     /locus_tag="VN034_14170"
     CDS             179..2914
                     /gene="secA"
                     /locus_tag="VN034_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWV61805.1"
                     /translation="MFSGLAKSLFGSSNDRYVTSIRKIVDKINAFEPAMQALDDAGLQ
                     AQTQKFRDQLAAGETLDDILPEAFATVREAAVRTLGMRHFDVQMVGGVVLHRGEIAEM
                     ATGEGKTLMATLPCYLNALEGKGVHVVTVNDYLARRDAEWMGTVYGFLGLTTGIIVPN
                     LNETQRRDAYNSDITYATNNELGFDYLRDNMKFDRQQMVHREFNFGIVDEVDSILIDE
                     ARTPLIISGPTDDKSELYIRVNEVVLQLGEEDYEKDEKSKSISLTEDGTEHVERLLEA
                     AGLLQGNNLYDIENTQVVHHVNQALKAIQMFRIDTDYIVKDGKVVIIDEFTGRMMDGR
                     RWSDGLHQAVEAKEGVQIEPENQTLASITFQNYFRMYPKLSGMTGTAATEAAEFFDIY
                     KMNVVNIPTNRPIARIDDEDEFYKNITDKFGAIAKTIREANERGQPVLVGTVSIEKSE
                     LLSSYLEKEGVPHSVLNARFHESEAHIVAQAGRTGAVTIATNMAGRGTDIKLGGNEEF
                     RIDDELKDLPEGAERDAGIARIQAEVAAEREAVLAAGGLFVLATERHESRRIDNQLRG
                     RSGRQGDPGLSKFYLCLDDDLLRIFGPDTLFSKMMNKNLEDGEAIGSKWLSKAIETAQ
                     KKVEARNYDIRKQVVEYDNVMNDQRKVIYEQRGEIIDSETVDEVMTAMRAETVNSIVA
                     DACPPGSYPEQWDVEAMKERVANILDLAPPIDDWMQEDAVDAEIFEERIQQAADAVAA
                     EKAGLVDAETWKGIEKSILLQTLDHHWKEHLSTLDALRQVVFLRAYAQKQPINEYKQE
                     AFALFERMLSNIREDVTRTVARIDLRFEEPEPMPLPDLPDFLTTHIDPFTGEDNSGDL
                     DDGQLGVIANTLPPMQIPRPDLPEGENPYAALEISRNAPCPCGSGRKYKHCHGAI"
     gene            3247..4686
                     /locus_tag="VN034_14175"
     CDS             3247..4686
                     /locus_tag="VN034_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWV61806.1"
                     /translation="MLRGKGWRIHFFDEMTGQEGEVRSPYREYSDWFGREDAARIQRK
                     TQQAEAFFRTTGITFNVYGEDEADERLIPFDVVPRIIAASEWRRLSRGIEQRVRALNA
                     FLHDIYHRQEILRAGRVPVELISRNEAFLPMMMGMDPPGGVYTHISGIDIVRTGADEY
                     YVLEDNARTPSGVSYMLENRETMLQMFPELFAKISVREVGDYPLNLLKSLAACAPPAC
                     NGTPTVAVLTPGIHNSAYFEHSFLADQMGAELVEGHDLRVVDGRVAMRTTQGYTPIDV
                     LYRRVDDDFLDPLNFRPDSVLGVPGIWDVYRAGAITIANAPGTGIADDKALYSYMPDI
                     IEFYTGEKALLPNVPTWRCSEPEHLREVLDRLPELVVKEVHGSGGYGMLVGPAASKKE
                     IAAFRAKLEVNPRNYIAQPTLSLSTVPIFTKAGLAPRHVDLRPFVLMSPQGIRITPGG
                     LTRVAMRKGSLVVNSSQGGGTKDTWVLED"
     gene            4880..5824
                     /locus_tag="VN034_14180"
     CDS             4880..5824
                     /locus_tag="VN034_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HWV61807.1"
                     /translation="MLGKTAGSLYWMARYLERSENNARLIDAGFRIALTRSSTARSEW
                     RSVLVTAGQDFAFRQAHGEDYTSQRVVDFLLRDPANPSSILSVVKRARDNARTARTYM
                     TREVWEAVNTSWMTLGALLKRQVREDDLPDVLAAIRRQSAQVGGTFSGTMLRNDGYNF
                     ARLGTFLERADNTARILDVKYYLLLPSVAHIGSSIDNVQWETILRSVSAHRAYHWLYG
                     TEISALKIAEFLILYKQMPRSLAFCCDQMNENLGLLQRSYGEEAEAVRLGTAISRDRL
                     SRPIASIFDGGLHEFITDFLRANAALARQIERDYRFVD"
     gene            5827..6630
                     /locus_tag="VN034_14185"
     CDS             5827..6630
                     /locus_tag="VN034_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWV61808.1"
                     /translation="MKLRVEHITHYHYDSPVRYALQQVKLTPKERPGQQLIHGWTIAV
                     DGGVQQLHYTDHHGNGVDLIAVDGGTSELVIRCTGEVELVTWDGVIGPHRGAMPLWTF
                     LRPTPLTRAGRGVRSLTAELGRDFANDIERAHALSARILDKLPYRLGVTDAETTAEQA
                     LAGEGGVCQDHAHIFIAAMRHLGHPARYVSGYLMMNDRTHQDATHGWAEAHFDHIGWI
                     GFDVSNGHSPDRRYIRVATGLDYHDAAPVRGMRYGAAQENLVVQLQVQQ"
     gene            6678..7409
                     /locus_tag="VN034_14190"
     CDS             6678..7409
                     /locus_tag="VN034_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HWV61809.1"
                     /translation="MTYCVGMRLNKGLVFMSDTRTNAGVDDISQVRKMRSWHVPGERV
                     ITLMSAGNLATTQAVVSLLDERTKAPAERQPSILQAPSMFQVATIIGETLRSIVMRHY
                     DEGPSAESLFRASLIVGGQIKGAEPRLFLIYPEGNFIEAGEDNPFFQIGETKYGRPII
                     VRSYDPAMSFEDSVKLLCVSFDSTIRANAGVDLPIDLKIHERDDFTTVRERRFERNDP
                     YFESVANGWAEALGIALAELPDFHF"
     gene            complement(7442..9406)
                     /gene="acs"
                     /locus_tag="VN034_14195"
     CDS             complement(7442..9406)
                     /gene="acs"
                     /locus_tag="VN034_14195"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HWV61810.1"
                     /translation="MSELSPSEPLVPVPADAAANTHCSAADYDRLYAQSVADPDAFWA
                     EQAKRIDWMTPPTKIAGWSYDPVEIKWYEDGVLNLCHNAVDRHVAAGHGDRTAIIFEP
                     DAPDGETRTISYAALLADVIRFANTLKKMGVRKGDRVTIYMPMIPEGAVAMLACARIG
                     AVHSVVFGGFSPEAIHGRLEDCASDWVICADEGLRGGKTVPLKANVDKALERVDVKAV
                     LVIAHTGGDVAMKEGRDHWYDALSADVPADCPCEPMNAEDPLFILYTSGSTGKPKGVL
                     HTVGGYSVWTASTFYYGFDYRPGEIFWCSADIGWVTGHSYVVYGPLQNGATTLMFEGV
                     PNYPDHDRFWQVVDKHRVNILYTAPTAIRALMREGDDYVTRHDLSSIRLLGSVGEPIN
                     PEAWRWYHQIVGKGRVPVVDTWWQTETGGIMITTLPGAHAMQPGSAGKPFFGIRPQLV
                     DAEGGLLVDEQTGGASEGNLCITHSWPGQARTIYGDHQRFAETYFSTYRGKYFTGDGC
                     RRDADGYWRITGRVDDVINVSGHRMGTAEVESALVLHEDVAEAAVVGFPHDIKGQGIY
                     AYVTLNAGVEPTDEVAAALKQQVRKEIGPIATPDHIHLTPGLPKTRSGKIMRRILRKI
                     AENDFGSLGDTSTLADPSLVDGLIEGRKNR"
     gene            9504..>10180
                     /locus_tag="VN034_14200"
     CDS             9504..>10180
                     /locus_tag="VN034_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWV61811.1"
                     /translation="MTVLGQEVARLLDGLGVDRALWADGSMPSVTPLTGEQLGMVQVV
                     DAATIDETLDKASAAFRAWRHVPAPRRGELVRLWGEELRAAKDDLAKLVTIEAGKIAS
                     EGAGEVQEMIDICDFAVGLSRQLYGLTIATERPGHRMMEVWHPLGVVGVISAFNFPVA
                     VWAWNDALALVCGNSVVWKPSEKTPLTALATQAIFERAVARFGDAPEGLAQLLIGGRE
                     AGEALVE"
BASE COUNT         1867 a         3193 c         3362 g         1758 t
ORIGIN      
        1 gccgcgcaag gcggctccgc cgcatcttcc cggcctgctg accggaagcg accgcgcgct
       61 cgaatagcgc cggctccttc gcgccttttt gagcgcgccg ccccgcgatg gggccccgtt
      121 tcctgattat cgcggcgcga cacgccgcca tttcaatctc atttccaagg aaaattcgat
      181 gttttctggc cttgccaaga gcctgttcgg ctcgtccaac gaccgttatg tcacctcgat
      241 ccgcaaaatc gtcgacaaga tcaacgcctt cgagcccgcg atgcaggcgc tcgacgatgc
      301 cggactgcag gcgcagacgc agaaattccg cgaccagctc gcggccggcg aaacgctcga
      361 cgatattctg cccgaagcct ttgccacggt gcgcgaggcc gcggtccgca cgctcggcat
      421 gcgccatttc gacgtccaga tggtcggcgg cgtcgtcctt caccgcggcg agatcgccga
      481 aatggcgacg ggcgaaggca agacgctgat ggcgaccttg ccctgttacc tcaatgcgct
      541 cgagggcaag ggcgtccatg tcgtcaccgt caacgactat ctcgcgcgcc gcgacgccga
      601 atggatgggc accgtctacg gcttccttgg cctgacgacc gggatcatcg tcccgaacct
      661 caacgagacg cagcgccgcg atgcctataa cagcgacatc acctatgcga cgaacaacga
      721 actcggcttc gattatctgc gcgacaatat gaaattcgat cgccagcaga tggtgcaccg
      781 cgaattcaac ttcggcatcg tcgacgaggt cgactcgatc ctgatcgacg aggcgcgcac
      841 gccgctgatt atctcggggc cgaccgacga caagtccgag ctgtatatcc gcgtcaacga
      901 ggtcgtcctc cagctcggcg aagaggatta tgagaaggac gaaaagtcga agtcgatcag
      961 cctgaccgaa gacggcaccg agcatgtcga gcggctgctc gaagccgcgg ggctgctcca
     1021 gggcaacaac ctctacgaca tcgagaacac gcaggtcgtc catcacgtca accaggcgct
     1081 gaaggcgatc cagatgttcc ggatcgacac cgactatatc gtcaaggacg gcaaggtcgt
     1141 gatcatcgac gagttcaccg gccgcatgat ggacggacgc cgctggtccg acggcctgca
     1201 ccaggcggtc gaggcgaagg agggcgtaca gatcgagccc gagaaccaga ccctcgcgtc
     1261 gatcaccttc cagaattatt tccgcatgta tccgaagctt tcgggcatga ccggcaccgc
     1321 ggcgaccgaa gcggccgaat tcttcgacat ctacaagatg aatgtcgtga acatcccgac
     1381 caaccgcccg atcgcgcgca tcgacgacga ggatgaattc tacaagaata tcaccgacaa
     1441 gttcggcgcg atcgcgaaga cgatccgcga agcgaacgag cgcggccagc cggtgctcgt
     1501 cggcaccgtg tcgatcgaaa agtcggaact cctgtcgtcg tacctcgaaa aggaaggcgt
     1561 gccgcacagc gttttgaacg cccgcttcca cgagagcgag gcgcatatcg tcgcgcaggc
     1621 gggccgcacc ggcgcggtga cgatcgcgac gaacatggcg ggccgcggta ccgacatcaa
     1681 acttggcggc aacgaagaat tccgcatcga cgacgagctc aaggacctgc ccgaaggcgc
     1741 cgaacgtgac gccggcatcg cgcgcatcca ggccgaagtc gccgccgaac gcgaggcggt
     1801 gctggctgcg ggcgggctgt tcgtgctcgc gaccgagcgc cacgaaagcc gccgtatcga
     1861 caaccagctc cgcggccgct cgggacgtca gggcgacccc ggtctgtcga aattctacct
     1921 ctgcctcgac gacgatttgt tgcgtatttt cggccccgac acgctcttct ccaagatgat
     1981 gaacaagaat ctggaggatg gcgaggcgat cggatcgaaa tggctgtcga aggcgatcga
     2041 gaccgcgcag aagaaggtcg aggcgcgcaa ttacgacatc cgcaagcagg tggtcgaata
     2101 tgacaacgtc atgaacgacc agcgcaaggt catctacgaa cagcgcggcg agatcatcga
     2161 cagcgagacc gtcgatgagg tgatgacggc gatgcgcgcc gaaacggtca actcgatcgt
     2221 tgccgacgcg tgccccccgg gcagctatcc cgaacaatgg gacgtcgagg cgatgaagga
     2281 gcgcgtcgcg aacatcctcg acctcgcgcc gccgatcgac gactggatgc aggaagatgc
     2341 ggtcgacgcc gaaatcttcg aagagcggat ccagcaggcg gccgatgcgg tcgccgccga
     2401 gaaggcgggc ctcgtcgatg ccgagacgtg gaaggggatc gaaaagtcga tcctgctcca
     2461 gacgctcgac catcactgga aggagcatct ctcgacgctc gacgcgctgc gtcaggtcgt
     2521 gttcctgcgc gcctatgcgc agaagcagcc gatcaacgag tataaacagg aagccttcgc
     2581 gctgttcgag cgcatgttgt cgaacatccg cgaggatgtg acgcgcaccg tcgcgcggat
     2641 cgacctgcgc ttcgaagagc ccgagccgat gccgctgccc gacctgcccg atttcctgac
     2701 gacgcatatc gatccgttca ccggtgagga caacagcggg gatctcgacg acggtcagct
     2761 gggcgtgatc gccaatacgc tgccgccgat gcagattccg cgaccggacc tgcccgaagg
     2821 cgaaaacccc tatgccgcgc tcgaaatcag ccgcaatgcg ccgtgcccgt gcggttcggg
     2881 gcgcaagtac aagcattgcc acggggcgat ctgacgctat ccctttcttc ccccttgatg
     2941 ggggaaggat atggagcctt atcgcgcagc gattaggcgg aattggatgg ggtgatggtg
     3001 cgtccttgcg cggccgagtc aggccgaaag cgcaccccca ccgctgcgac tagcgaacaa
     3061 gttcgcaagt ctcgctgcct ccccaatcaa gggggaagat tgtcgacaat taccgtgctt
     3121 gccaaggtgg atagccgcga ttagcatcgc cccataagct tgagggccgc gtagccagac
     3181 aagcgacgca gcaggcagga actatggggc cgcgcacgcc cgaagcatcg cggccggacc
     3241 ggtgacatgt tgcgtggaaa gggctggcgc attcattttt tcgacgagat gaccggacag
     3301 gagggcgagg tccggtcgcc gtatcgcgaa tattcggact ggttcggccg cgaggacgcg
     3361 gcgcgcatcc agcgcaagac tcagcaggcc gaggcctttt tccgcaccac cggcatcacc
     3421 ttcaacgtct atggcgagga tgaagccgac gagcggctga tccccttcga cgtcgtgccg
     3481 cggatcatcg cggcgagcga atggcggcgg ctgtcgcgcg gcatcgaaca gcgcgtgcgc
     3541 gcattgaacg ccttcctcca cgacatctat caccggcagg agattttgcg cgcgggccgc
     3601 gtgccggtcg agctgatctc gcgcaacgag gctttcctgc cgatgatgat gggcatggac
     3661 ccgccgggcg gcgtctatac ccacatcagc ggcatcgaca tcgtgcgcac cggcgccgac
     3721 gaatattatg tgcttgagga taatgcgcgc acgccgtcgg gcgtctccta catgctcgag
     3781 aatcgcgaga cgatgcttca gatgttcccc gagctgttcg cgaagatttc ggtgcgcgag
     3841 gtcggcgact atccgctcaa cctcctgaag tcgctcgcgg cctgcgcgcc gcccgcctgc
     3901 aacggcacgc cgacggtcgc ggtgctgacc cccggcatcc acaacagcgc ctatttcgag
     3961 cacagcttcc tcgccgacca gatgggcgcc gaactggtcg aggggcatga tttgcgcgtt
     4021 gtcgacggcc gcgtcgcgat gcgcacgacg cagggctata cgccgatcga cgtcctctac
     4081 cgccgcgtcg acgacgattt cctcgatccc ctgaacttcc ggccggactc ggtgctcggc
     4141 gtgccgggga tctgggacgt ctatcgcgcc ggcgcgatca cgatcgccaa cgcgcccggc
     4201 accggcatcg ccgacgacaa ggcgctctat agctacatgc ccgacatcat cgaattctat
     4261 acgggcgaga aggcgctgct gcccaatgtg ccgacctggc gctgctccga acccgagcat
     4321 ttgcgcgagg tgctcgaccg gctgcccgag ctggtggtga aggaagtcca cggatcgggc
     4381 ggctacggga tgctcgtcgg cccggcggcg agcaagaagg agatcgcggc attccgcgcc
     4441 aagctggagg tgaacccgcg caattatatc gcgcagccga cgctgtcgct ctcgaccgtg
     4501 ccgatcttca ccaaggcggg gctcgccccg cgccacgtcg acttgcggcc cttcgtgctg
     4561 atgtcgccgc aggggatccg gatcacgccg ggcgggctga cgcgcgtcgc gatgcgcaag
     4621 gggtcgctgg tggtgaattc gagccagggc ggcgggacca aagacacctg ggtcctggag
     4681 gattgatggg ggtgcggctc caattcatct cctatcccct cccgcaagcg ggaggggcag
     4741 cgaggcttgg cagcttgctg cctagccgca gcggggtggg tcggccacgc tgcgttcttg
     4801 cccaccccgc tgcgactagc gaacaagttc gcaagtctcg ctgcccctcc cgcttgcggg
     4861 aggggacggg ggtgcgtcca tgctagggaa gaccgccggc tcgctctact ggatggcgcg
     4921 ctatctcgaa cggagcgaaa acaatgcgcg gctgatcgac gcggggtttc gcatcgcgct
     4981 gacgcggtcg agcaccgcgc ggtcggaatg gcggtcggtg ctcgtcaccg cggggcagga
     5041 tttcgcgttc cggcaggcgc atggcgagga ttatacctcg cagcgcgtcg tcgatttcct
     5101 gctgcgcgat cccgccaatc cgtcgagcat attgtcggtg gtcaagcgcg cgcgcgacaa
     5161 tgcccgcacc gcgcgcacct atatgacccg cgaggtgtgg gaggcggtca acacgagctg
     5221 gatgacgctc ggcgcgctgc tcaagcggca ggtgcgcgag gatgatttgc ccgacgtgct
     5281 cgcggcgatc cggcggcaga gcgcgcaggt cggcgggacc ttttcgggca cgatgctgcg
     5341 caacgacggt tataatttcg cgcggctcgg caccttcctc gaacgcgccg acaacaccgc
     5401 gcgcattctc gacgtcaaat attatctgct gttgccgtcg gtcgcgcata tcggcagctc
     5461 gatcgacaat gtgcagtggg agacgatcct gcgctcggtg tcggcgcacc gcgcctatca
     5521 ctggctctat gggaccgaga tcagcgcgct gaagattgcc gaatttctga tcctctacaa
     5581 gcagatgccg cgcagtctcg ctttctgctg cgatcagatg aacgagaatc tcggactgct
     5641 ccagcgcagt tatggcgagg aggccgaggc ggtgcgattg ggcacggcga tcagccgcga
     5701 ccggctctcg cgtccgatcg cgtcgatctt cgacggcggc ctgcacgagt tcatcaccga
     5761 tttcctccgc gccaatgccg cgctcgcgcg gcagatcgag cgcgattacc gttttgtgga
     5821 ctgacgatga aactacgcgt cgaacatatc acccattatc attacgacag cccggtccgc
     5881 tacgcgctgc agcaggtgaa gctgacgccg aaggagcggc cggggcagca gctgatccac
     5941 ggctggacga tcgcggtcga cggcggcgtg cagcagcttc actataccga tcatcacggc
     6001 aatggcgtcg acctgatcgc ggtcgacggc gggacaagcg agctcgtcat ccgctgcacc
     6061 ggcgaagtcg aactggtgac ctgggacggc gtgatcggtc cgcaccgcgg cgcgatgccg
     6121 ctctggacct ttctgcggcc gaccccgctg acgcgcgcgg gacgcggggt gcggtcgctg
     6181 accgccgagc tggggcgcga tttcgcgaac gacatcgaac gggcgcatgc gctgtcggcg
     6241 cggatcctcg acaagctgcc gtaccggctc ggggtcaccg acgccgagac gaccgccgag
     6301 caggcgctcg cgggcgaggg cggggtgtgc caggatcacg cgcatatctt catcgccgcg
     6361 atgcgccatc tggggcaccc ggcgcgttac gtctcgggct atctgatgat gaacgaccga
     6421 acgcatcagg atgcgacgca tggctgggcc gaagcgcatt tcgaccatat cggctggatc
     6481 ggcttcgacg tgagcaacgg ccattcgccc gaccgccgtt atatccgggt cgcgacgggg
     6541 ctcgattatc acgacgccgc gccggttcgc gggatgcgct atggcgcggc gcaggaaaat
     6601 ctggttgtcc aattgcaggt ccagcaataa gcggaccggg aacctcaggg ctaaccgggc
     6661 ggggaatacg ggattcgatg acttattgcg ttggcatgcg tttgaacaag gggctggtgt
     6721 tcatgtcgga cacccgcacc aacgcgggcg tcgacgacat ctcgcaggtc cgcaagatgc
     6781 gcagctggca cgtgccgggc gagcgcgtga tcacgctgat gtcggcggga aatctcgcga
     6841 cgacgcaagc ggtcgtcagc ctgctcgacg aacgtaccaa ggcaccggcc gagcggcagc
     6901 cctcgatcct tcaggcgccc tcgatgttcc aggtcgcaac gatcattggc gaaacgctgc
     6961 gcagcatcgt gatgcgccac tatgacgagg gcccctcggc cgaatcgctg ttccgcgctt
     7021 cgctgatcgt cgggggccag atcaagggcg ccgaaccccg gctgttcctg atttatcctg
     7081 aaggcaattt catcgaggcg ggagaggata accccttctt ccagatcggc gagaccaaat
     7141 atggccggcc gatcatcgtg cgatcctatg atcccgcgat gtcgttcgag gattcggtga
     7201 aattgctctg cgtgtcgttc gattcgacga tccgggcgaa tgccggcgtc gacctgccga
     7261 tcgacctgaa aatccatgag cgcgacgatt tcaccacggt gcgcgagcgc cgcttcgagc
     7321 gcaacgaccc ctatttcgaa agcgtcgcga acggctgggc cgaggcgctg gggatcgcgc
     7381 tggcggagct gccggatttt catttctaag ttcccctccc gcttgcggga ggggagaaag
     7441 atcaccggtt cttccgcccc tcgatcagcc cgtcgaccag gctcggatcg gcgagcgtcg
     7501 acgtatcgcc cagcgatccg aaatcattct cggcgatctt gcgcaaaatc cgccgcatga
     7561 tcttgcccga gcgcgtcttg gggagccctg gcgtcagatg gatatggtcg ggggtcgcga
     7621 tcgggccgat ttccttgcgc acctgctgct tgagcgccgc ggcaacctcg tcggtcggtt
     7681 cgaccccggc gttgagcgtg acataggcgt agatgccctg acccttgatg tcgtgcggga
     7741 agccgaccac cgcggcctcg gcgacatctt cgtgaaggac gagcgcgctt tcgacctcgg
     7801 cggtgcccat gcggtggccc gagacgttga tcacatcgtc gacgcggcct gtgatccgcc
     7861 aatagccgtc ggcgtcgcgg cggcagccgt cgccggtgaa atatttgccc ctgtaggtcg
     7921 agaaataggt ttcggcgaag cgctggtggt cgccgtagat cgtgcgcgcc tgccccggcc
     7981 agctgtgcgt gatgcacaga ttgccttcgg atgcgccgcc ggtctgttcg tcgacgagca
     8041 agccgccctc ggcatcgacc aactgcggac ggatgccgaa gaagggcttg cccgcgctgc
     8101 ccggctgcat ggcgtgcgcg ccgggcaggg tggtgatcat gatcccgccg gtttcggtct
     8161 gccaccaggt gtcgacgacg gggacgcggc ccttgccgac gatctggtga taccagcgcc
     8221 acgcctcggg attgatcggt tcgccgacgc tgccgagcag gcggatcgac gacaggtcat
     8281 gccgcgtcac ataatcgtcg ccctcgcgca tcaacgcgcg gatcgcggtc ggcgcggtgt
     8341 agaggatgtt gacccgatgc ttgtcgacga cctgccagaa gcggtcgtga tccggataat
     8401 tgggcacgcc ctcgaacatc agcgtcgtcg cgccgttctg gagcgggccg tagacgacat
     8461 aggagtggcc ggtgacccag ccgatgtcgg cgctgcacca gaagatttcg ccgggacggt
     8521 agtcgaagcc ataatagaag gtgctcgcgg tccacacgct gtagccgccg acggtgtgga
     8581 gcacaccctt gggcttgccc gtcgagcccg aggtgtagag gatgaagagc gggtcctcgg
     8641 cgttcatcgg ctcgcacggg cagtcggcgg gaacgtccgc ggacaaggcg tcataccaat
     8701 ggtcgcggcc ttccttcatc gcgacgtctc cgccggtgtg cgcgatgacg agcacggcct
     8761 tgacgtcgac gcgctcgagc gccttgtcga cattggcttt cagcggaacg gtcttgccgc
     8821 cgcgtaatcc ctcgtcggcg cagatcaccc agtcgctcgc gcaatcctcg agccggccgt
     8881 ggatcgcctc gggcgagaag ccgccgaaga cgacactgtg caccgcgccg atccgcgcgc
     8941 aggcgagcat cgcgaccgcg ccttcgggga tcatcggcat atagatggtg acgcggtcgc
     9001 ctttgcggac gcccattttc ttcagcgtgt tcgcgaagcg gatgacgtcg gcgagtagcg
     9061 cggcatagct gatcgtccgc gtctcgccat cgggggcgtc gggttcgaag atgatcgcgg
     9121 tgcggtcgcc gtgccccgcg gcgacatggc ggtcgacggc gttgtggcaa aggttgagaa
     9181 cgccgtcctc ataccatttg atctcgacgg ggtcgtagga ccagccggcg atcttcgtcg
     9241 gcggcgtcat ccagtcgatg cgcttcgcct gttcggccca gaaggcgtca ggatcggcga
     9301 cgctttgcgc gtagagccgg tcgtagtcgg cggcgctgca gtgcgtgttc gcggcagcgt
     9361 cggcggggac ggggaccagc ggttcggacg gcgacaattc ggacacggac gactctccaa
     9421 tcggctttgc attgttcgcc cgctgcgata acccgcgagc cacaaactgc aagggcgcgt
     9481 cggcgccaat gaagggacag atgatgacgg tattgggcca ggaggtcgcc cggttgctcg
     9541 atggactcgg cgtggaccgc gccctgtggg ctgatggatc gatgccgtcg gtgacgccgt
     9601 tgaccggcga gcagctcggg atggtgcagg tcgtcgatgc cgcgacaatc gacgagacgc
     9661 tcgacaaggc gagcgcggcg ttccgtgcat ggcgccatgt tccggcgccc cggcgcggcg
     9721 agctggtgcg cttgtggggc gaggaattgc gcgcggcaaa ggacgatctg gccaagctgg
     9781 taacgatcga ggcgggcaag atcgcgtccg aaggcgcggg cgaagtgcag gagatgatcg
     9841 acatctgcga cttcgcggtc gggctttcgc ggcaattata tggcctgacc atcgcgaccg
     9901 agcgaccggg gcaccggatg atggaggtct ggcatccgct gggtgtcgtc ggagtgatct
     9961 cggcgttcaa tttccccgtc gcggtgtggg catggaatga cgcgctcgcg ctggtgtgcg
    10021 gcaacagcgt cgtgtggaag ccatcggaaa agacgccgct tacggccttg gcgacgcagg
    10081 cgattttcga gcgcgcggtc gcgcggttcg gcgatgcgcc cgaaggcttg gcgcagctgc
    10141 tgatcggcgg ccgcgaggcg ggcgaggcgc tggtcgaatt
//
LOCUS       DATMSU010000530        11014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_132364,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000530 DATMSU010000000
VERSION     DATMSU010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132364"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VN034_14205"
     CDS             complement(<1..281)
                     /locus_tag="VN034_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HWV61812.1"
                     /translation="MVGGDINMKAIDVPVQRAQVENLYVEAMLLADEAHAAFAAQRDL
                     GSVRGNAATQIGLACESLKTTTRLMHVIAWLLHRRAMFAGDPGAGPNDSA"
     gene            complement(372..569)
                     /locus_tag="VN034_14210"
     CDS             complement(372..569)
                     /locus_tag="VN034_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HWV61813.1"
                     /translation="MSPEEITQRLELLRIEHRDLDAAIIALGEGTTTDQLQIARLKKR
                     KLKLRDEIAWCEDQLLPDIIA"
     gene            693..851
                     /locus_tag="VN034_14215"
     CDS             693..851
                     /locus_tag="VN034_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HWV61814.1"
                     /translation="MSTSHLSTLKSRHADLDVKIANEERRPAPDTGVVAQLKKRKLKL
                     KEEMLAIG"
     gene            complement(894..1256)
                     /locus_tag="VN034_14220"
     CDS             complement(894..1256)
                     /locus_tag="VN034_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWV61815.1"
                     /translation="MDEEVAALSRGADRDSLFMQAELALPGGGSVTVRVRNLSPGGML
                     AEGKVTVAQGAAVEVDLRNVGPVAGRVIWVGEGKFGIAFDRAIDPQAVRRQVVSQSDL
                     PPHLRRTGLERGPLYRRR"
     gene            complement(1365..1826)
                     /gene="dksA"
                     /locus_tag="VN034_14225"
     CDS             complement(1365..1826)
                     /gene="dksA"
                     /locus_tag="VN034_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HWV61816.1"
                     /translation="MPTKIADVGADALREARSNLDSDYVPRDDEEFMNERQQDYFRGL
                     LLAWKKSILSESESTLAHLQDGPLREPDLADRASSETDWAIELRTRDRQRKLIAKIDS
                     AIRRIEEGEYGYCAVTGEPISLARLQARPIATMTLEAQERHERNERISRED"
     gene            complement(2170..2487)
                     /locus_tag="VN034_14230"
     CDS             complement(2170..2487)
                     /locus_tag="VN034_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61817.1"
                     /translation="MRITFFVVTTRRTRFTGLRAAWRLRTTFFLATQGEQDWTCARAF
                     GTSVICIAPLPMIAPPQVQAQSFARAIRTDIGFSFFPVAGAKRHRPTPPITFAITQKD
                     KAL"
     gene            complement(2542..3324)
                     /locus_tag="VN034_14235"
     CDS             complement(2542..3324)
                     /locus_tag="VN034_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61818.1"
                     /translation="MRFAARRRATSAAAAAPNNSIIGGAGTGVGSPDELLVPPELDEE
                     ELDDELLLDDELVLPVEPKLLLPPVAPLDVLLLDEEDDEELLDDELLPEPPDPPEVPC
                     DEPPQPELPFDEPPDEEELDEEDDEEELDDELLLDDDVLTLPVEVDTLPDDVETLPEE
                     VLTLPVEVETPPVEVDTPPVEVDTLPVEVETPDEVLVEVDTPPVVVEVTTMLPLLPPP
                     ELPPKKPPKKPPPNPPPKPPPTTVTPPPLPPPPSQPDCGKGA"
     gene            3480..3824
                     /locus_tag="VN034_14240"
     CDS             3480..3824
                     /locus_tag="VN034_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWV61819.1"
                     /translation="MAKQQAALDSVFHALADPTRRAVVNRLLKGAAPVKRLSEPFQMG
                     LPAFMKHLSVLEASGLITTEKQGRVRTCRVDAERLAAAEGWLSEQRAIWQGRTDRLAA
                     FVESQNPPEEKS"
     gene            3821..4279
                     /locus_tag="VN034_14245"
     CDS             3821..4279
                     /locus_tag="VN034_14245"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWV61820.1"
                     /translation="MSKSELTISRHIAAPPSAVWDAWRDPAKLAQWWIPAPIECRVDK
                     LDLTPGGGFVTRMREGDGDFGPHVDGCFLEAIPNERLVFTTVLTEGWQPAEPWLALTA
                     ILTFEAKDGGTLYSARVLHKTPEESAKHEEMGFYEGWGTAIGQLAALLER"
     gene            4322..5596
                     /gene="serS"
                     /locus_tag="VN034_14250"
     CDS             4322..5596
                     /gene="serS"
                     /locus_tag="VN034_14250"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWV61821.1"
                     /translation="MHDIRLIRDDPQAFDAGLARRGLEPLSAEILAADASLRALQTEI
                     QAALARRNEASKLIGQAMAGGDKDKAEALKAEVAELKTALPAKEEAEREQLAGLHDRL
                     AALPNIPAADVPDGEDEESNVEISRWGTPRTFDFAPKEHADFAPALGLDFETAAKMSG
                     ARFAFLKGQMARLERALGQFMIDRQTNEAGYTECATPLMVRDEAAFGTTQLPKFREDL
                     FQTTDGRWLISTSEMSLTNAVREEILPEADLPIRMTALTPCFRSEAGSAGRDTRGYIR
                     QHQFWKVELVSIVRPEDSDAELERKTRAAEQILEALDLPYRKMLLCSGDMGFAARKTY
                     DLEVWLPGQDSYREISSCSNCGDFQARRMNTRFRREDVKGNEFVHTLNGSGLAVGRTL
                     VAILENYQQADGSVDIPKALLPYMGGITRLIP"
     gene            5704..6468
                     /gene="surE"
                     /locus_tag="VN034_14255"
     CDS             5704..6468
                     /gene="surE"
                     /locus_tag="VN034_14255"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWV61822.1"
                     /translation="MRILLTNDDGYHAPGMAVLEAIARQLSDDIWVCAPAEEQSGAGH
                     SLTLSRPVRIREHDERRWSCSGTPTDSVMMAIGKLMPEKPDLILSGVNRGANLGDDVT
                     YSGTVSAAIEGALAGIPAIALSQVYAREGMGDSVPFEAAEAWGAKVLRPLLGMDMAPR
                     TLININFPALPAAEVKGIRVTRQGFHDYGRGSIVEGTDPRGYRYYWFGLHGIEHSLGH
                     DSDLEAIDDQYISVTPLQLDLTHDASLAALRGAYGG"
     gene            6502..7569
                     /locus_tag="VN034_14260"
     CDS             6502..7569
                     /locus_tag="VN034_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HWV61823.1"
                     /translation="MSKSPASKLKLQHRFQPLRRMSKWPIWADVLTRLGVAFLLIAIV
                     VLVHWIDRAGLRDSHDGQISFLDVVYFTMISVTTTGFGDITPVSDRSRLIEAVIVTPI
                     RIMVLFIFVGTAYQFAIKQIWEKWRMARIQAKLTNHIVVLGFGISGSEAVRELIARGT
                     DPACIVVIDQARNRINAAEAMGCNVLEGDASSDETLIDVHIARAQSVLVSAGRDDTSI
                     LMVLTVRHLAPRVPISVVIRTQDNELLARQAGANNVINPVSFTGLLLAGSAQGAHVAD
                     YMADLASVGGRVQLRERPVAAEEVGRSLDQLASEGRGLRIYRGGKPFGFWEDEAQSLQ
                     HGDLIVEVIRCEEAEASVPGG"
     gene            complement(7580..7909)
                     /locus_tag="VN034_14265"
     CDS             complement(7580..7909)
                     /locus_tag="VN034_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HWV61824.1"
                     /translation="MAIGAFAAISAAMAVAAGAFGAHGASGPQEAEWLRTGGVYQLVH
                     AVAALALMGFARGPAITLLGGAAIFAITLYAMALGAPRWLGAVTPIGGGLLIIGWLWA
                     GWLYWRG"
     gene            8003..9373
                     /gene="rimO"
                     /locus_tag="VN034_14270"
     CDS             8003..9373
                     /gene="rimO"
                     /locus_tag="VN034_14270"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWV61825.1"
                     /translation="MTVKTLPTQPKVGMVSLGCPKALVDSERILTKLRADGYGLSPDY
                     AGADVVLVNTCGFLDSAKEESLEAIGEAMAENGRVIVTGCMGNEADVIRARFPNVLAV
                     TGAHQYEQVVDAVHDAAPPTQGPFIDLVPEGGLKLTPRHYSYLKISEGCNHSCSFCII
                     PDLRGKLVSRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDIRHDPRPWHGREVRA
                     HMTDLARELGQLRTSEGNAPWVRLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASP
                     SVLKRMKRPANEAKVLERLKSWRQVAPDISIRSSFVVGFPGETEADFQYLLDWLDEAQ
                     LDRVGAFRFEPVAGAQANALDDQVPEEVKEERYQRIMAKTAAISAAKLEAKIGRTLPV
                     IIDEVGEADEDGSIGATGRSQADAPEIDGHVYLRDVAASLRAGDIVNVEIEDADEHDL
                     FGAIAD"
     gene            9440..10912
                     /locus_tag="VN034_14275"
     CDS             9440..10912
                     /locus_tag="VN034_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HWV61826.1"
                     /translation="MHAKAFAALLLIGSLTGCDRKTAVEAPPRATDKAPSPKQTSLIA
                     VPIDASTASLRQALERAVPKTLWTINRRERACVKPQRVKLFGKKVKVTPPIACTIVGQ
                     VTRGALRLRGEGDEFVVDVPLRARISARDVGGVLKGETATGAAMAHARVRIDLTPDWR
                     TLGKARITYGWTKAPGIDFLGRRITFTEEADAKLKPVVASVEREVNREVAKIDIRGQA
                     ADIWRQAFTALELNRENPPVWMRVTPQRILYGGYRLDGQRINLNLGLEAITETFVSGR
                     PADPAPTPLPRLVRETPKPHFDVRVPVIADYAQLQPVVDRALAKRAARPFELPKVGPM
                     MVEFGKSTIYGASGGRIAIGVDVDAKLEARTGAPTRGRIWMTAIPVNEPGSAEVRFTG
                     LVINGDTDGVGGDLLILLGRSPEFAPLIAAALTQNFTHDLEELQGKIKRAVDQRREGA
                     FLIRTRVDRFETGAIKAYGNGLYLPVRMVGGASVDYRPAK"
BASE COUNT         1951 a         3770 c         3523 g         1770 t
ORIGIN      
        1 gcgctgtcgt tcggcccggc gcccgggtcg cccgcaaaca tcgcccggcg gtggagcagc
       61 caggcgatga cgtgcatcag ccgcgtcgtc gtcttcagcg attcgcaggc gaggccgatc
      121 tgcgtcgccg cgttgccgcg aacgctgccg aggtcgcgct gcgcggcaaa ggccgcatgc
      181 gcctcatcgg cgagcagcat cgcctcgaca tagagatttt cgacctgcgc gcgctgcacc
      241 ggcacatcga tcgccttcat gttgatgtcc ccgccgacca tgtctgacgc gatgccacgc
      301 aacgcggcgg gtccgcaatc gcgctaacca tcatttgccg cggctgtccc gttttgattc
      361 aggcggttgg ttcaggcgat gatgtcgggc agcagttgat cctcgcacca ggcgatctcg
      421 tcgcgcagct tgagcttgcg ctttttcagc cgcgcgatct gcaactggtc ggtcgtcgtc
      481 ccttcgccga gcgcgatgat cgcggcgtcg aggtcgcgat gctcgatgcg aagcagttcc
      541 aggcgctggg taatttcctc ggggctcacc cggcctccct gccacgttca ccactggtcc
      601 gcaacgggga ccgccccacc gaatcgtcac gacatcgcgg cgctttcatg ttttacttcg
      661 ccctgcccat cgagtcgaaa aggagaatgc caatgagcac gtcgcacctt tccaccctga
      721 agtcccgcca cgcggacctc gatgtgaaaa ttgcgaatga agagcgccgt cccgcccccg
      781 ataccggagt cgtcgcgcag ctcaagaagc ggaaactgaa actcaaggaa gagatgctcg
      841 cgatcggctg atcgccagcg ctatcgcccc gctgatgcag ccctacaggg ccatcagcgc
      901 cggcggtaga ggggccccct ctcaaggccc gtgcgccgca gatgcggcgg caaatcggac
      961 tgcgaaacca cctgacgacg aaccgcctgc ggatcgatcg cgcggtcgaa ggcgatgccg
     1021 aacttgcctt caccgaccca gatcacgcgc cccgcgaccg gcccgacgtt gcgcaggtcg
     1081 acctcgaccg cggccccctg cgcgacggtc accttgccct cggcgagcat gccgccgggc
     1141 gacaggttgc gcacgcggac ggtgacggac ccgccgccgg gcagcgccag ctctgcctgc
     1201 atgaacaggc tgtcgcgatc ggcaccgcgc gacagtgccg cgacttcctc gtccaccgat
     1261 gtcggtcttt gcgaatccat ttgcgccccg tttctctcgt atcgaggcgc ggaatctagg
     1321 gtccagcggt tgccgaaacg taaatggggc gtcagagccc gcgatcaatc ttcgcgtgaa
     1381 atccgttcgt tacgctcgtg gcgctcctgc gcctccagcg tcatcgtcgc gatcggccgt
     1441 gcctgcaacc gcgcgagcga gatcggctcg ccggttaccg cgcaatagcc gtattcgccc
     1501 tcttcgatgc ggcggatcgc cgaatcgatc ttggcgatca gcttccgttg acggtcgcgg
     1561 gtgcggagct cgatcgccca gtcggtctcg ctcgacgcgc ggtcggcgag gtcgggttcg
     1621 cgcagcgggc catcctgaag atgcgcgagc gtcgattccg attcggacag gatcgatttt
     1681 ttccaggcga ggagcaggcc gcggaaataa tcctgctgcc gctcgttcat gaattcctcg
     1741 tcgtcgcggg ggacataatc cgaatcgaga ttggacctgg cttcacgaag cgcgtctgcg
     1801 ccaacatcgg caatcttggt cggcatgagg ggctctcgcg tcctttcctg tcacccggcg
     1861 ggccgcaagc ggctcgggga gtggcgcgcc tatacccagc caccggccgc gacacaagcg
     1921 ccgaagctgc cgatagcggc cgggcgtgaa gcggcgatga accgtccgca gcttgagggt
     1981 gattccggtg ccccgtccga tcgcatcgac cttcacaggg agacggcgat ttaggtccga
     2041 aggttaacca atcgcataga attgctgcat aatccgtgct gttttgggac agataacgcg
     2101 ttttttgttg attccgcggt ccttttcccg ctctcggaca ggcaaacgcg gttaacagaa
     2161 ttgccgcgtt cacaaagctt tgtccttttg agttatagcg aaggtgatgg ggggtgtcgg
     2221 tcgatggcgc ttcgcacctg caacgggaaa gaaagagaag cctatgtctg tccgaatggc
     2281 ccttgcgaag ctttgcgcct gcacctgcgg cggggcgatc atcggtagcg gcgcgatgca
     2341 gatcaccgac gttccaaagg cgcgggcgca ggtccagtcc tgctcgccct gcgtcgccaa
     2401 gaaaaaggtc gtgcgcaaac gccacgcggc gcgcaagccg gtgaagcgcg tccgccgcgt
     2461 cgtcaccacg aagaaggtga tccgcaccgc gaccccgcag acgcaggtgg tgacgcagac
     2521 gatccccctg cccccgatcc cctacgcgcc cttcccgcaa tcgggctggg aaggcggcgg
     2581 cggtagcggc ggcggcgtga cggtcgtcgg cggaggcttt ggcggcgggt tcggcggcgg
     2641 cttcttcggc ggtttcttcg gcggcagctc gggcggtggc agcagcggca gcatcgtcgt
     2701 cacctcaacg acgacgggcg gcgtgtcgac ctcgaccagc acctcgtcgg gggtctcgac
     2761 ctcgacgggc agcgtgtcga cctcgaccgg cggcgtgtcg acctccacgg gcggcgtttc
     2821 gacctcgacc ggcagcgtca gcacctcctc gggcaacgtc tcgacatcgt cgggcaatgt
     2881 gtcgacctcg accggcagcg tcagcacgtc gtcgtcgagc agcagttcgt cgtcgagttc
     2941 ctcttcgtcg tcctcttcgt cgagttcctc ctcgtcgggc ggctcgtcaa aaggcagctc
     3001 gggctggggc ggctcatcgc acggcacttc ggggggatcg ggcggttcgg gaagcagctc
     3061 gtcgtcgagc agctcctcgt catcctcctc gtcgagcagc agcacgtcga gcggcgcgac
     3121 cggcggcagc agcagcttcg gctcgaccgg gagcaccagc tcgtcgtcga gcagcagctc
     3181 gtcgtcgagt tcctcctcgt cgagctcggg cggcaccagc agctcgtcgg gcgatccgac
     3241 ccctgttcct gcgccgccga tgatgctatt gttcggtgca gcagcagccg cgcttgtcgc
     3301 gcgccgccgc gcggcgaatc gcaacgacga cgacgccaac gcggcttgag aaatccactg
     3361 gcggtcaggg gggctatccc ggaccaccga actaagggct gcccccctcg gggcggccct
     3421 tttttggacc cgtgcaaatc agtttgccat atggagaact attacatata gttcaccata
     3481 tggcgaagca acaggcagct ctcgatagtg ttttccatgc actcgccgac ccgacgcgac
     3541 gcgccgtcgt caaccggctg ctcaagggcg ccgccccggt aaagcggctg tccgaaccgt
     3601 ttcaaatggg ccttcccgcc ttcatgaaac atctctcggt gcttgaggcg tcggggctga
     3661 tcaccaccga aaaacagggc cgtgtccgca cctgccgcgt cgatgccgaa cgactcgccg
     3721 ccgccgaggg ctggctctcg gagcaacgcg ccatctggca gggccgcacc gaccgcctcg
     3781 ccgcattcgt cgaatcgcaa aatcccccgg aggaaaaatc atgagcaaaa gcgaactcac
     3841 catctcgcgc cacatcgccg caccgccatc ggccgtgtgg gacgcgtggc gcgaccccgc
     3901 aaagctcgcg caatggtgga tcccggcgcc gatcgaatgt cgcgtcgaca agctcgacct
     3961 cacccccggc ggcggcttcg tgacgcggat gcgcgagggc gacggcgatt tcgggccgca
     4021 tgtcgacggc tgtttcctcg aagcgatccc gaacgaacgc ctcgtcttca cgaccgtcct
     4081 caccgaaggc tggcagcccg ccgagccctg gctcgcgttg accgcgatcc tgacctttga
     4141 agcgaaggac ggcggcacgc tctattcggc gcgcgtgctg cacaagacgc ccgaagaaag
     4201 cgcgaagcac gaagaaatgg gtttttacga aggctggggc acggcgatcg gccagctcgc
     4261 ggcactgctg gagcggtaag acgggcttgc accgcccgcg ccgcaacgcc atagcgccgc
     4321 catgcacgat atccgcctga tccgggatga cccgcaagcc ttcgacgccg gcctcgcgcg
     4381 ccgcggcctc gaacccttgt ccgccgagat tcttgccgcc gacgcatcgc tccgcgcgct
     4441 gcaaaccgag attcaggccg cgctcgcgcg ccgcaacgag gcgtcgaagc tgatcggtca
     4501 ggcaatggcg gggggcgaca aggacaaggc cgaggcgctg aaggccgaag tcgcggaact
     4561 caagaccgcg ctgcccgcga aggaagaggc cgagcgcgag cagctcgccg ggctccacga
     4621 caggctcgcc gcactcccca acattcccgc cgccgacgtt cccgacggcg aggatgagga
     4681 gagcaatgtc gaaatttcgc gctggggcac cccgcggacg ttcgacttcg caccaaagga
     4741 acatgccgac ttcgcccccg cactcgggct cgatttcgag accgcggcca agatgtcggg
     4801 ggcgcgcttc gctttcctga agggccagat ggcgcggctc gaacgcgcgc tcggccagtt
     4861 catgatcgac cggcagacga acgaggcggg ctataccgaa tgcgcaacgc cgctgatggt
     4921 gcgcgacgag gccgcgttcg gcacgacgca gctcccgaaa ttccgcgagg acctgttcca
     4981 gaccaccgac ggccgctggc tgatctcgac gtccgagatg agcctgacca acgcggtgcg
     5041 cgaggaaata ttgcccgagg ccgatctgcc gatccgcatg accgcgctca ccccctgctt
     5101 ccggtccgaa gcgggatcgg cgggacgcga tacgcgcggt tatatccgtc agcatcagtt
     5161 ctggaaggtc gagctcgtct cgatcgtccg ccccgaggat tcggacgccg agctcgagcg
     5221 caagacgcgc gcggccgagc aaatcctcga ggcgctcgac ctgccgtacc gcaagatgct
     5281 gctgtgcagc ggcgacatgg gcttcgcggc gcgcaagacc tatgacctcg aggtgtggct
     5341 gccggggcag gacagctacc gcgagatttc gagctgctcg aactgcggcg acttccaggc
     5401 gcggcgcatg aacacgcgct tccggcgcga ggacgtaaag ggcaatgagt tcgtgcatac
     5461 gctcaacggc tcgggcctcg cggtcgggcg gacgctggtg gcgatcctcg aaaattatca
     5521 gcaggccgac ggcagcgtcg atattccgaa ggcgctgctg ccctatatgg gcggcattac
     5581 ccgcctcatc ccctagtccg ttcgtgctga gcttgtcgaa gcaccgtcct tcttttcgac
     5641 gtccgaaaga aagaacggcc cttcgacagg ctcagggcga gcggaattga gaaagaccga
     5701 aacgtgcgca tcctcctcac caacgacgac ggctatcacg cgcccggcat ggcggtgctg
     5761 gaggcgatcg cgcgccagct ttctgacgat atctgggttt gcgcccccgc cgaggaacag
     5821 tcgggcgcgg gccactcgct caccttgtcg cgcccggtac ggatccgcga acatgacgag
     5881 cgacgctggt cgtgcagcgg caccccgacc gattcggtga tgatggcgat cggcaagctg
     5941 atgcccgaaa agcccgacct cattctctcg ggcgttaacc gcggcgccaa cctcggcgac
     6001 gatgtcacct actcgggcac cgtgtcggcg gcgatcgagg gcgcgctcgc aggcatcccg
     6061 gcgatcgcgc tcagccaggt ctatgcgcgc gagggcatgg gcgacagcgt gccgttcgag
     6121 gcggccgaag cctggggcgc gaaggtgctg cgtccgctgc tcggcatgga catggcgccg
     6181 cgcacgctga tcaacatcaa cttccccgcc cttcccgccg ccgaggtcaa gggcatccgc
     6241 gtgacgcggc agggcttcca cgactatggc cgcggctcga tcgtcgaggg gaccgatccg
     6301 cgcggatacc gctattactg gttcggcctg cacggcatcg aacacagcct cggccatgac
     6361 agcgatctcg aggcgatcga cgatcaatat atttcggtca ccccgctcca gctcgacctg
     6421 acgcacgacg cctcgctcgc cgcgctgcgc ggcgcttatg gcggctagcg ttacgcgcgc
     6481 ggctttggca caagggccgc gatgagcaaa agccccgcgt ccaagctgaa gctccagcac
     6541 cggttccagc cgctccgccg gatgagcaaa tggccgatct gggccgacgt cctcacgcgc
     6601 ctcggcgtcg cctttctgct catcgccatc gtcgtgctcg tccactggat cgatcgggcc
     6661 gggctcaggg acagtcacga cggccagatc agctttctcg acgtcgtcta tttcacgatg
     6721 atctcggtca cgacgaccgg cttcggcgac atcaccccgg tatccgaccg ctcgcggctg
     6781 atcgaggcgg tgatcgtcac cccgatccgc atcatggtgc tcttcatctt cgtcggcacc
     6841 gcctatcagt tcgcgatcaa gcaaatatgg gaaaaatggc gtatggcccg catccaggca
     6901 aagctcacca accatatcgt cgtcctcggc ttcggcatca gcggcagcga ggcggttcgc
     6961 gaactgatcg cgcgcggcac ggaccccgcg tgcatcgtcg tgatcgacca ggcgcggaac
     7021 cggatcaacg ccgccgaagc gatggggtgc aacgtgctcg agggcgacgc gtcgagcgac
     7081 gagacgctga tcgacgtgca tatcgcccgc gcgcagtcgg tgctcgtctc ggcgggccgc
     7141 gacgatacct cgatcctgat ggtgctcacc gtgcgccacc ttgcgccgcg cgtgccgatc
     7201 agcgtcgtca tccggacgca ggacaatgag ctgctcgcgc ggcaggcggg cgcgaacaat
     7261 gtcatcaatc cggtcagctt caccggcctg ctgctcgcgg gatcggcgca gggcgcgcat
     7321 gtcgccgact atatggccga cctcgcgagc gtcggcgggc gcgtccagtt gcgcgagcgc
     7381 ccggtcgccg ccgaagaggt tggccgcagc ctcgaccagc tcgccagcga agggcgcggc
     7441 ctccgcatct atcgcggcgg caagcccttt ggcttttggg aagacgaggc gcagagcctc
     7501 cagcatggcg acctgatcgt cgaggtcatc cgctgcgagg aagccgaagc gagcgtgccg
     7561 ggcggctgac cgggcgaggc tagccccgcc agtagagcca gcccgcccag agccagccga
     7621 tgatcagcag gccaccgccg atcggcgtca ccgcgccgag ccagcgcggt gcgcccagcg
     7681 ccattgcata aagggtgatc gcaaagatcg ccgccccgcc aagcaacgtg atcgccggcc
     7741 cgcgcgcgaa tcccatgagg gcgagcgccg ccaccgcgtg gaccagctga tacacgccgc
     7801 cggtgcgcag ccattcggcc tcctgcggtc ccgaagcgcc atgcgcgccg aacgcgcccg
     7861 ccgcgaccgc catcgccgcc gaaatcgccg caaatgctcc gatcgccata tgctcaaccc
     7921 tttcatctcg aaccgcgccg tccatgcccg cgtgcgcggc gcgtgacaag ggccgccaaa
     7981 tcgactatgg gcgccgccaa tcatgacagt caaaacgctc cccacccagc cgaaggtcgg
     8041 catggtttcg ctcggctgcc ccaaggcgct cgtcgacagc gaacggattc tgaccaagct
     8101 gcgcgccgac ggttacggcc tctcgcccga ctatgcgggc gccgacgtcg tgctcgtcaa
     8161 cacctgcggc ttcctcgatt cggcgaagga agaaagcctc gaggcgatcg gcgaagcgat
     8221 ggccgagaac ggccgcgtca tcgtcaccgg ctgcatgggg aacgaagccg acgtcatccg
     8281 cgcgcgcttc ccgaacgtgc tcgcggtcac cggcgcgcat cagtatgagc aggtcgtcga
     8341 cgcggtccac gacgccgcgc cgccgacgca gggccccttc atcgatctgg tacccgaagg
     8401 cgggctgaag ctgacgccgc gccactacag ctatctgaag atcagcgaag gctgcaacca
     8461 cagttgctcc ttctgcatca tccccgacct gcgcggcaaa ctcgtcagcc gccgcatcga
     8521 cgcggtgttg cgcgaagccg aaaagctcgt cgccgcgggg acgaaggaac tgctggtgat
     8581 cagccaggac acctcggcct atggcgtcga catccgtcac gacccgcgtc cgtggcacgg
     8641 ccgcgaagtg cgcgcgcaca tgaccgacct cgcgcgcgaa ctcggccagc tccgcaccag
     8701 cgaaggcaat gccccgtggg tgcgcctcca ctatgtctac ccctatccgc acgtcgatgc
     8761 ggtgatcccg ctgatggccg agggactgct caccccctat ctcgacatcc ccttccagca
     8821 cgcgagcccg agcgtcctca agcgcatgaa gcgccccgcg aacgaagcga aggtgctcga
     8881 acggctgaag agctggcgcc aggtcgcccc cgacatttcg atccgctcga gcttcgtcgt
     8941 cggcttcccc ggtgagaccg aggcggattt ccagtatctt ctcgactggc tcgacgaagc
     9001 gcagctcgac cgcgtcggcg ccttccgctt cgaacccgtc gcgggtgcgc aggccaatgc
     9061 gcttgacgat caggtgcccg aagaggtcaa ggaagagcgc tatcagcgga tcatggccaa
     9121 gaccgccgcg atcagcgccg cgaagctcga agccaagatc ggccgcacgc tcccggtcat
     9181 catcgacgaa gtcggcgagg ccgatgaaga cggcagcatc ggcgccaccg gccgcagcca
     9241 ggccgacgcg cccgaaatcg acggccacgt ctatctccgc gacgtcgcgg cgagcctcag
     9301 ggccggcgat atcgtcaatg tcgagatcga ggacgccgac gagcatgatc tgttcggcgc
     9361 gatcgctgac taacggataa tattggaacg gctttacttg ttcgatgtta cctatgcgac
     9421 atcgaacagg ggccgcacca tgcatgccaa ggcgtttgcc gcactgctat tgatcgggtc
     9481 gctcactggc tgcgaccgca agacagcggt cgaagcgcca ccccgcgcca ccgacaaggc
     9541 cccctcccca aagcaaacat cgctgatcgc ggtgccgatc gacgccagca ccgcatcgct
     9601 caggcaggcg ctcgaacgcg cggtgcctaa aacgctgtgg acgatcaacc ggcgcgagcg
     9661 cgcgtgcgtc aagccgcagc gggtcaagct cttcggcaag aaggtcaagg tcaccccgcc
     9721 gatcgcctgc acgatcgtcg gacaggtgac gcgcggggcc ctgcggctgc gcggcgaagg
     9781 ggacgagttt gtcgtcgatg tgccgctccg tgcccggatc agcgcgcgcg acgtcggcgg
     9841 agtgttgaaa ggcgagaccg cgaccggcgc ggcgatggcg catgcccgcg tccgcatcga
     9901 cctgaccccc gactggcgca ccctgggcaa ggcgcgcatc acctatggct ggaccaaggc
     9961 gccgggaatc gacttccttg ggcgtcgcat caccttcacc gaagaggccg acgcgaagct
    10021 caaacccgtg gtggcgagcg tcgagcgcga agtgaatcgc gaggtcgcga aaatcgacat
    10081 ccgggggcag gccgccgaca tctggcggca ggcctttact gcgctcgaac tcaaccgcga
    10141 aaacccgccg gtgtggatgc gcgtcacgcc gcagcggatt ctctatggcg gctaccggct
    10201 cgacgggcag cggatcaacc tcaacctagg gctcgaggcg atcaccgaaa cctttgtttc
    10261 ggggcgcccg gccgatcccg cgcccacgcc gctcccccgc ctcgtgcgcg aaacgccgaa
    10321 accgcatttc gacgtgcgcg tgcccgtgat cgccgactat gcgcagttgc agccggttgt
    10381 cgaccgcgcg ctcgccaagc gggcggcgcg tcccttcgag ctgccgaaag tcggtccgat
    10441 gatggtcgaa ttcggcaagt cgacgatcta tggcgcctcc ggcggacgga tcgcgatcgg
    10501 cgtcgatgtc gatgcgaagc tggaggcgcg gaccggcgcg ccgacacgcg ggcgcatctg
    10561 gatgaccgcg attcccgtca acgagccggg ttcggccgaa gtccgcttca ccggcctcgt
    10621 gatcaacggc gataccgacg gggtcggcgg cgacctgctc atcctgctcg gccgcagccc
    10681 cgaattcgcg ccgctgatcg ccgcggcgct gacgcagaac ttcacgcacg atctggaaga
    10741 gttgcaggga aagatcaaac gcgcggtcga tcagcgccgc gagggcgcct tcctcatccg
    10801 cacgcgcgtc gaccgcttcg aaaccggcgc gatcaaggcc tatggcaacg ggctgtacct
    10861 gccggtgcgg atggtcggcg gtgccagcgt cgattaccgt ccggcgaaat gatcagaaaa
    10921 ggcgcgtgat cgcatagaag acagcggcga ccgccgcgct cgcgggtatg gtgatgaacc
    10981 aggccgcgac gacattgccc gcgacgcccc agcg
//
LOCUS       DATMSU010000531         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_9439, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000531 DATMSU010000000
VERSION     DATMSU010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9439"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..570)
                     /locus_tag="VN034_14280"
     CDS             complement(151..570)
                     /locus_tag="VN034_14280"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV61827.1"
                     /translation="MTDTPPGNRPGKGQLIAGRVLSGLVILFLLFDAGLKLVSPETAI
                     KYSPPGLGWPLEVGTMHMLGLLLLIPTLLYIWPRTAVLGAILITGYLGGAIATHLRID
                     SPLFSHTLFGVYLGVMLWAGLWLRSPALRLLVTPRWA"
     gene            complement(596..1030)
                     /locus_tag="VN034_14285"
     CDS             complement(596..1030)
                     /locus_tag="VN034_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61828.1"
                     /translation="MPQMIFVNLPVTDLDKSKAFYEAVGAANNPAFTDETAACMVVAE
                     GSIHVMLLTHAKWADFTTKTIPDARTHAQVLLCVSADSREEVDGQVDKAVKAGGKADP
                     TPTQDFGFMYGRSFEDPDGHIWELMWMDPTAIPAGEPAEATA"
     gene            complement(1098..>1697)
                     /locus_tag="VN034_14290"
     CDS             complement(1098..>1697)
                     /locus_tag="VN034_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWV61829.1"
                     /translation="RGSARAKLIAAAHAMVRKQGYAATTVDQICAEAGVTKGAFFHHF
                     ASKEALAVAAAEGWTERARPMFEMPAHVKLDDPLARLLGHIDFRLAMLDGPTEDYTCF
                     VGTMVQEAYATSDAIRAACEASINAYCEALAPDIQATIDLYGAPEDVTAIGLAQHVQS
                     VLQGSFVLAKTTNDPAIARESVIHLKRYVRMLFGRGGAA"
BASE COUNT          284 a          532 c          580 g          301 t
ORIGIN      
        1 ggcgggctgc ggcagggcgg cgaccgccgc cgcgcccgcg attatcgcga aggtcgaggc
       61 aaggccgggg aggggagagc gtcgtgtcat cgaaccatcc tctttgcgtg taagacggag
      121 actaggcgcg tttacctttg agacaggcga tcaggcccat cgcggcgtga ccagcaagcg
      181 caatgcgggc gaacgtagcc acagacccgc ccagagcatc acgcccagat agacgccgaa
      241 caatgtgtgg ctgaacagcg ggctgtcgat gcgcagatgg gtcgcgatcg cgccgccgag
      301 atagccggtg atcaggatcg cgccgagcac ggcggtgcgc ggccagatat agagcaaggt
      361 cgggatcagc agcagcaggc cgagcatatg catcgtgccg acctcgagcg gccagccgag
      421 gccgggcggg ctgtatttga tcgccgtttc gggcgatacc agcttgagcc cggcgtcgaa
      481 gagaaggaag aggatcacaa ggccgctgag cacgcggccg gcgatcaatt gaccctttcc
      541 ggggcgattt ccggggggcg tatcggtcat cgtcttcctc ctccctatgc tttgcttatg
      601 cggtcgcttc ggcgggttcg ccggcgggaa tcgcggtggg gtccatccac atcaattccc
      661 agatatggcc gtcgggatcc tcgaagctgc ggccgtacat gaagccgaaa tcctgcgtcg
      721 gggtcgggtc ggccttgccg cccgccttga ccgccttgtc gacctggccg tcgacctctt
      781 cgcggctgtc ggccgatacg cagagcagga cctgcgcgtg ggtgcgcgcg tcggggatcg
      841 tcttggtcgt gaagtcggcc catttggcgt gggtcagcag catgacgtgg atcgagcctt
      901 cggcgacgac catgcacgcg gcggtctcgt cggtgaaggc ggggttgttc gccgcgccga
      961 ccgcttcgta aaaggccttc gacttgtcga ggtcggtgac cggcaggttc acgaaaatca
     1021 tctggggcat ttactctctc cttcgggggg tgttggaaat cagcgtgcgc ggtgcgcggg
     1081 ggcaattacc tgtcgcatca tgccgcgcct ccgcgtccga acagcatccg gacataacgc
     1141 ttcagatgga tgacgctttc ccgcgcgatc gccgggtcgt tcgtcgtttt ggccaggacg
     1201 aaactgccct gcagcaccga ctggacatgc tgcgcgaggc cgatcgcggt gacgtcctcg
     1261 ggggcgccat aaaggtcgat cgtcgcctgg atgtcggggg ccagcgcctc gcaataggcg
     1321 ttgatgctcg cctcgcacgc ggcgcggatc gcatcgctcg tcgcataggc ttcctgcacc
     1381 atcgtgccga cgaagcaggt ataatcctcg gtgggcccgt cgagcatcgc gaggcggaaa
     1441 tcgatatggc cgagcaggcg cgcgagcgga tcgtcgagct tcacatgcgc cggcatctcg
     1501 aacatcggcc gcgcgcgctc ggtccagccc tcggccgccg cgaccgccag cgcttccttc
     1561 gacgcaaaat ggtgaaagaa ggcgcccttg gtcacgcccg cttcggcgca gatctggtcg
     1621 accgtcgtcg ccgcatagcc ttgcttgcgc accatcgcat gcgccgccgc gatcagcttc
     1681 gcacgggcgc tgccacg
//
LOCUS       DATMSU010000532         1732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_179691,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000532 DATMSU010000000
VERSION     DATMSU010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179691"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VN034_14295"
     CDS             <1..189
                     /locus_tag="VN034_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61830.1"
                     /translation="CRCGDPHGKGVTGVAFRSGLPIQKIPLFDPATPIAPQTRGKAGA
                     APRARASAATRRIKLSTN"
     gene            462..>1732
                     /locus_tag="VN034_14300"
     CDS             462..>1732
                     /locus_tag="VN034_14300"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HWV61831.1"
                     /translation="MDFRETERVETSDVATAELTKNEAPVAPESTGESAPDAAPAAKL
                     NDSSEPKVHARRFEVVTDRSRDALLTDFGKETLEDRYLLPGESYQDLFARVADAYADD
                     QDHAQRLYDYISKLWFMPATPVLSNGGTGRGLPISCYLNSVDDSLEGIVGTWNENVWL
                     ASRGGGIGTYWGAVRGIGEPVGLNGKTSGIIPFVRVMDSLTLAISQGSLRRGSAACYL
                     DVSHPEIEEFLEVRKPSGDFNRKALNLHHGVLITDEFMEAVRDGTEFNLRSPKDQSVR
                     GTVDARSLFQKLVETRLATGEPYIIFIDQVNRMMPKHHRDLGLKVTTSNLCSEITLPT
                     GRDHLGNDRTAVCCLSSLNLETWDEWNGDKVFIEDVMRMLDNVLQDYIDRAPPEMARA
                     KYSASRERSVGLGVMGFHSFLQARGLPFEGA"
BASE COUNT          345 a          588 c          512 g          287 t
ORIGIN      
        1 tgccggtgcg gtgatccgca cgggaagggc gtcaccggcg tcgcctttcg ctccggtctt
       61 cccattcaaa aaattccgct ttttgatccg gcgacgccaa tcgctccgca aacccgcgga
      121 aaggctggcg cggcgcctcg cgctcgtgca tctgccgcga cgagacgaat caaattatcg
      181 acgaactgag tccccaatct gtcatttagc gtcttgcatc gcggcgccga cgtggcacta
      241 taggtggtgg gtcacacacg ggggcacccc aagaaatagt attcggctgc aactggcgga
      301 tttccgtcgt ccatcccata tggacacggg aaaaccctcc ctgcgcccaa aaaaagtgcc
      361 cacggggaca ggtttagatg ctaagaccgg ggcttcgccc tgagtcgaac aagacaggaa
      421 caaagcgcac cagttgcgcg ttcaaatcgg gggcgaataa gatggatttt cgggaaacgg
      481 aacgggtgga gacgagcgac gtggcgacgg cggaactgac gaagaatgaa gcccccgtgg
      541 cacccgaatc gacgggtgaa tcggctcccg atgcggcgcc cgcggcaaag ctcaacgaca
      601 gcagcgagcc caaggtccac gcgcgccgat tcgaggtcgt gaccgaccgc agccgcgacg
      661 cgctgctcac cgacttcggc aaggaaacgc tcgaggatcg ttacctgctg cccggcgaat
      721 cgtaccagga tctcttcgcg cgcgtcgccg atgcctatgc cgatgatcag gaccacgcgc
      781 agcgcctgta cgactatatc tcgaagctgt ggttcatgcc cgcgaccccc gttctctcga
      841 acggcggcac cggccgcggc ctgccgatca gctgttatct gaactcggtc gacgacagcc
      901 tcgaaggcat cgtcggcacg tggaacgaga atgtctggct cgcgagccgc ggcggcggca
      961 tcggcaccta ttggggcgcg gtgcgcggca tcggcgaacc cgtcggcctc aacggcaaga
     1021 ccagcggcat catccccttc gtccgcgtga tggactcgct caccctcgcg atcagccaag
     1081 gctcgctgcg ccgcggttcg gccgcctgct acctcgacgt ctcgcacccc gagatcgagg
     1141 agttcctcga ggtgcgcaaa ccctcgggcg acttcaaccg caaggcgctc aatctccacc
     1201 acggcgtcct catcaccgat gagttcatgg aggccgtccg cgacggcacc gaattcaacc
     1261 tccgctcgcc gaaggaccag agcgtccgcg gcaccgtcga cgcgcgttcg ctgttccaga
     1321 agctcgtcga aacgcgcctc gcgacgggcg agccctacat catcttcatc gatcaggtga
     1381 accgcatgat gccgaagcat catcgcgatc tcggcctcaa ggtcaccacc tcgaacctct
     1441 gctcggaaat cacgctgccc accggccgcg accatctcgg caacgatcgc accgcggtct
     1501 gctgcctctc gagcctcaac ctcgaaacct gggacgagtg gaacggcgac aaggtcttca
     1561 tcgaggacgt catgcgcatg ctcgacaatg tccttcagga ctatatcgac cgcgccccgc
     1621 ccgaaatggc gcgcgccaaa tatagcgcca gccgcgaacg ctcggtcggc ctcggcgtca
     1681 tgggcttcca cagcttcctg caggcgcgcg gcctgccgtt cgagggcgcg at
//
LOCUS       DATMSU010000533        16322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_150641,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000533 DATMSU010000000
VERSION     DATMSU010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 16322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16322
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150641"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..960)
                     /locus_tag="VN034_14305"
     CDS             complement(172..960)
                     /locus_tag="VN034_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HWV61832.1"
                     /translation="MIDSDATHAKPAPVSAGSIRHFRYYDLVMAAFVAVLLLSNIIGA
                     SKLSMVGGLTFGAGILFFPISYVIGDVLTEVYGYARARRVIWTGFAALLFMAVMSWIV
                     LAMPPAQDWWCSGTETLALKSGEAVGPGAVCQQTYESVFGAAWRIVLASMAAFWAGEF
                     VNSYVLARMKLATGGRHLWMRTIGSTIFGQGIDSLIFYPIAFLGIWETEQVFLVLATN
                     WAMKVAWEAVLTPATYVVVGALKRREGVDVFDEGTDFNPFGARV"
     gene            1031..1107
                     /locus_tag="VN034_14310"
     tRNA            1031..1107
                     /locus_tag="VN034_14310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1065..1067,aa:Arg,seq:tct)
     gene            complement(1263..2210)
                     /locus_tag="VN034_14315"
     CDS             complement(1263..2210)
                     /locus_tag="VN034_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61833.1"
                     /translation="MRLALSIFFSLIIATSSAQARIAYVPFVMKEDFGIRPVVDARID
                     DTAVALAVHSGAAGLIELTPEAADALGYHDRIYAGQYGIDRSGHLASAGASRVRLGTL
                     TIGPNITSPALAFVHALPGNKADILRSGKAGMLGIKWLRSSRAFVDFGGKRIGFPQEA
                     NDTASARARLTEAGYTALPMIWNEKEAAYTVNVRLSGHSANLIVNTVSAGYLDLPSSQ
                     AAGLVFGEQTGTAGGPKGATVPVYKPLAPLSIEIGGRHFPGFQPPAWDTGAYLSRSSA
                     AEGAPAPGPIQGALGATFWIARAAVIDFGGGWLFLAPSK"
     gene            complement(2371..2715)
                     /locus_tag="VN034_14320"
     CDS             complement(2371..2715)
                     /locus_tag="VN034_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61834.1"
                     /translation="MNNSAKTDAARERHQTLDLIGLRAHATAVGLIQLCEELLNAGVL
                     SAAGLDRIKDAICSELTVAKRARVSKQWDLDDTLRQRIDAVFPAASGAPARENVGPIE
                     DFEDALTGYHGA"
     gene            complement(2778..4001)
                     /locus_tag="VN034_14325"
     CDS             complement(2778..4001)
                     /locus_tag="VN034_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWV61835.1"
                     /translation="MQFDVVIVGAGHGGAQVAVMLRTQKFEGSIAIIGEEPELPYERP
                     PLSKEYFAGEKEFERIQLRPARYWDEREVTMLLGRRVVSVDPAGHSVTTGDGETIGYG
                     KLVWATGGAPRMLPIPGGDLPGVQGVRTRADADAMKAASESAEQIVVIGGGYIGLEAA
                     AVLTKAGKKVVLLEALDRVLARVAGVELSRFYEKEHREHGVDLRLGVCVDAIEGDDHV
                     TGVRLSDGEVIPADLVIVGIGIVPAVEPLTAAGADCANGVRVDPLCKTSLADIYAIGD
                     CASHTNDFAEGAEIRLESVQNANDQANVVAKGIAGDEAPYHAIPWFWSNQYDLKLQTA
                     GLSTGHDQAVLRGDPANRSFSVVYLKAGKVIAIDCVNATRDYVQGRMIVTAGLRATPE
                     QLADPETPLKALLPA"
     gene            4160..4897
                     /locus_tag="VN034_14330"
     CDS             4160..4897
                     /locus_tag="VN034_14330"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61836.1"
                     /translation="MQDDTNFTGRHVLVVGGSSGIGNGIAHGFRIRGAEVHVWGTRAA
                     ASDYDPGEGSDLTGLGYSCVDVGDSAAIEAAAAPFETLDALILCQGTVVYGRGEFERD
                     GWDRVMAVNLDSLMHIARKFRGQLAQARGSIVIVSSVSGLRGNIGNPAYAASKAGAIS
                     LTRSLGQAFASDGIRVNGLAPGLVDTKLTKATTSHPERLEKSLASIPQRRMGTPDDMA
                     GAAIFLASALASYVTGHTLVVDGGLTL"
     gene            complement(4914..6143)
                     /locus_tag="VN034_14335"
     CDS             complement(4914..6143)
                     /locus_tag="VN034_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009824064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HWV61837.1"
                     /translation="MSRRIGMAEPGAAMCQASLVFDDAVAILSNAANALGHESVALGA
                     AAGRRLAEPVVARIDGPRWDCAAMDGYALRDGDLAAGLRRFASLGEIVAGDVALTAVT
                     PGSTMRVMTGAPLPDGADRVVMIEYCTKIGGMVEIRGDPGSKPHVRRRGSDFVAGQTL
                     LNKGRELTPVALVAAAAGDVSHVTVWRRPRVHIIATGDELVPPGEAANRADRIPDSLT
                     AAIALLSGQWGGCVVRETRIGDDPRAIRDAFSKEDDVTVIIGGASRGDRDFGRSALVP
                     VGLRLLFADVAIKPGKPSWYGKVGEAHVLGLPGNPTAALTVARLFLAPLLTGLGGGEP
                     RSALAWTHLRAAREVEAQGEREAFLIGDIVDGAALLLDRQEASAQNLLGQATILVRRP
                     ARAPRCAAGDALAVLAI"
     gene            complement(6165..7064)
                     /locus_tag="VN034_14340"
     CDS             complement(6165..7064)
                     /locus_tag="VN034_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWV61838.1"
                     /translation="MAYDQIRLDKHEGIAVVTLHRPERMNAFTVEMMHEIIAAIDECD
                     ADDGVRAVIFTGHGDRAYCAGADLSAGASTFDYEKQGGASGGPIAADGTIDWSHDLVR
                     DGGGHVSLRIFDAKKPVIGAINGAAVGVGVTMTLPMDARLASDNARFGFVFARRGLVP
                     EAASSWFLPRIVGIANAVDLCYSGRLISAAEALDKGLVQAVHQPGELLDAAIARAREM
                     TADSAPVSVALTRHMLWRMMGAPHPMSAHRWDSRVIFARGRGPDAAEGVSSFLEKRPP
                     AFAASVANDYPWFAEFEEAPPYA"
     gene            complement(7198..8805)
                     /locus_tag="VN034_14345"
     CDS             complement(7198..8805)
                     /locus_tag="VN034_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWV61839.1"
                     /translation="MDFDYIICGAGAAGCVLAYRLSEDPNVRVALIEAGPRDRHPFIS
                     MPKGLAKVMQDPKHLWVHMSEPEASTAGQSEAWVRGRVLGGSSSVNGMMYVRGQPADF
                     DAIAEKSSDDWSWTHIGAAYRALENHELGGAETRGDKGPLKISMPTQRLPISEAQVAA
                     GEAMGWANKPDVNAPDDEVAIGYAPRTIFKGKRQSAAQAFLRPAEKRPNLTILTERTV
                     DRVRFDGARAVGVEVVHNGVRETIGAAHEVILCGGAMASPAILERSGVGDSNLLARLG
                     IPPVHHNPEVGEGLIEHRGIIMQWKLTRQALSQNRVFGGCRLLLATLRYYLTGDGPMS
                     AAAYEIGGWFKTRPGLNRPDAQMLIAPFSFDMATQRTQLEKHPGMNAVIYPLRPTSRG
                     SIHIATRDPDVAASFKPNYRSTDEDRAAMVGAVRVMRDYAKRAPLASMIAEETMPGPA
                     FGSDAEILDAYDRFGTCGYHAVGSCRMGKDDGSVVDPALRVRGVEGLRIIDTSVMPVI
                     PSGNTNGPTMAMAWRAADIILRDRRPG"
     gene            8924..10102
                     /locus_tag="VN034_14350"
     CDS             8924..10102
                     /locus_tag="VN034_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWV61840.1"
                     /translation="MSDVAEVSRPARKVTTLDDLTPSQIEAIRKIPAEKDAVVVPLGA
                     TRPNAIFTGRERFDLEQEKIFRRYPVPVTVSALLEPGSVVANDSYGFPMLVSRTRDGT
                     IKAFVNACQHKGSKIIEDCAVHKQGRMTCPYHAWTYGIDGKLIGVSRSEAFEGFDKSQ
                     RGLAELEAREWGGIVYVQLNRDIPADWSHLSDQVAEDFGALGIPSAHVYGRKTFDLKA
                     NWKVVLEPFLEGYHVQRLHAASIGDKFQDAPNIVDMFGANIRQVSGRIGYVPEMLDED
                     PLANVHKLVTHAYTAFPNCVIVTSQYYTSVMLLMPRDADRTTVEYFMLTPEAAATPKA
                     EEVFARSYELILGVFGGEDFRAAEISQQGLNAGVPEETVYCGLETNIIRYYEAIEALL
                     "
     gene            10137..11636
                     /locus_tag="VN034_14355"
     CDS             10137..11636
                     /locus_tag="VN034_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWV61841.1"
                     /translation="MAQTLSGALAWWARMRPEQPAVVLGGDRLGYGDYKAWSDRVAAM
                     LEADGLRPGDRVAICSTNSLAYCALLMGVIRAGGIANPVNFRFTPREIRELCETSEPR
                     FTFAAPDFEANVIAAGLTPRAMAEIEALRDGGPATVGHDPDPDAPVVIIATSGSTAKP
                     KGVVFTNRSMTAYAANWAVEETASSPGARVISLAPLNTSAGFVQLMHYTVQGCTIFME
                     PVFAPDAILRLLQDEKINCFGAVPTFFEAIARCPGFADADLSSIRLATAGGARVSRQL
                     LDAYKAKGVTIRQIYGQTEIGGNATMMPEHLAMDEPEKCGWGCVFMDLRVVRPDGSDC
                     DPGEPGEIVMRSPGMMQGYWRNPEETAKVIRDGWLWSGDIGTLDERGLLTFVDRMKDL
                     IISGGLNISAAEVERVVCEFPGVVEALAIAAPDEKFGETPFVVYHGAAEVDVRALIDH
                     CNVNLSNYKVPRYVAFSVDPLPRLATGKLSKPAVRALYAGAHETMERVR"
     gene            11647..12867
                     /locus_tag="VN034_14360"
     CDS             11647..12867
                     /locus_tag="VN034_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWV61842.1"
                     /translation="MTTAVLAAAPVPPHVPAHLVFDFDIYADPRIGEDVQGTYAAALS
                     DAPDIFWTRLNGGHWIVKSFDAISQVVLDPEHFSVREMQIPRVENPPFFIPLSLDPPE
                     NLPYRRAMMPMFGPAAIKALEPRIREWAVELVDAVATKGACDFQADLAKLFPVSVFME
                     LMGMDLSRLRDFRALAEAFFSAQNDEAEMGRLGAAILGEMKALIEAKKAAPDDKLMSH
                     FITVDIDGRRMSDDEILAMSFVLFLGGMDTVTNVTGFAFQQLAQMPEVQARLAADPSL
                     IPAFADEAVRLYGVVNTPRLVVRDQEIGEAKFREGEMLLNILCLGSRDPTKFEAPNMF
                     DLDRRKTAHLTFSSGPHLCVGHVLGRAELRILTEEWVKRIPRFRAVPGEKHGFRIGTV
                     MALESLPIEWDVAG"
     gene            complement(12869..13510)
                     /locus_tag="VN034_14365"
     CDS             complement(12869..13510)
                     /locus_tag="VN034_14365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61843.1"
                     /translation="MARPSKPLISRSNAAEAALEVIDEVGLEELSLSLVGRKLGVRPP
                     SLYHHFKDKSELLQEVARMMFVRIPTVNDPALSFEEQIIARCVVTRRALLKHPNAAPL
                     MLRYFPRHLLLAAYERAVAEEPYPASIHLTVIDAIEKYTFGTALFEASAQVRGIPPIP
                     SVDAAKYPNFAKAVADNPFGDEELFVESLRMFLVGVRQRLATETIGKPLDQIR"
     gene            13655..14542
                     /locus_tag="VN034_14370"
     CDS             13655..14542
                     /locus_tag="VN034_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV61844.1"
                     /translation="MTNRLQRKTFVSPDGVRLVGDHGGPIDAPCAVLMHGGGQTRHSW
                     SRAVERLLDAGLQVINFDARGHGDSDWSPGGAYALTDRAADLAAVTADLHVPFILIGA
                     SLGGATSIEAVHEGLRPAGVVLVDIVPAPDPRGIERIVGFMHGHQGGFASVDEAAAAV
                     AAYNPQRERPRDTRGLMKNLRPRDDGRLYWHWDPRILETAPAIHHAAVARAADTLAAL
                     TDIPVLLVRGLSSDVVSEAGVDAFRRQMPRLEILDVLGAGHMVAGDRNDAFNDGVIAF
                     AKRLLVSEALSSSSSIDLK"
     gene            14668..>16322
                     /locus_tag="VN034_14375"
     CDS             14668..>16322
                     /locus_tag="VN034_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61845.1"
                     /translation="MDRNVTRPAIRYACLASVIALAAVAPPAFAQTDPAPPPPADEAA
                     AGSMADSEIIVTARRRDERLIDAPVAITALGGAALDNYAVTEFNDIAQLVPTMVAGKA
                     ASGSSASIFLRGVGSTALSAGFDQSVSFVVDGMPMSRGREIGLSQYDVQRVEVLKGPQ
                     ALFFGKNATAGLIAVTTNSPRDVFEAGLKIGYGFEAQEKYGEGYISGPLADGLRARLA
                     FRISDSEGAFTNTAAETYIDPFGLERHRNSKNRGYGRTYSGRATIEWDAADNLTFQVK
                     TGYTDQKDGGPTDIIERICGGGRTVPFAANGIPPSPNADCVIDGRSDSSTIPIEVAQL
                     DYRYAGDGRMYSRLKSGFGILTATLTSDVFDVTSITSYYRFKQNDLNNVSGEAYPATF
                     SQLADYRQTSEELRFQSKWDGPINALFGLFVSSNKFIFNTDAYIFPLPAVAAGTTYVT
                     FKRNNGFSGSSMSAFGEVTANLTDSLELAGGARWSYESRDSFQESLAANPAFAAAFPG
                     GLRLDDRYRETDVSPQVTLRYKPSRDLTFYAAYKEGFKSGGFN"
BASE COUNT         2852 a         5343 c         5233 g         2894 t
ORIGIN      
        1 cgcgagccac gcggccagcc acccgccgcg ccagcgccac gccagccgta ccgccacgaa
       61 cggcgcgagc ccgaaataga tatgcagcgc gtccttgctc agcccggccg aggtgacgag
      121 ccacgtcttc tgttcggtga acagcgcggc gagctcgatc aacgtcatgg actacactct
      181 ggcgccaaag gggttgaaat cggtgccttc gtcgaacacg tcgacacctt cgcggcgctt
      241 caacgcgccg acgacgacat aggttgccgg ggtcaagacg gcctcccacg cgaccttcat
      301 cgcccagttg gtggcaagga ccaggaacac ctgttcggtc tcccagatgc cgaggaaggc
      361 gatggggtag aagatcaggc tgtcgatgcc ctgcccgaag atcgtcgagc cgatcgtgcg
      421 catccacagg tggcgccccc cggtcgcgag cttcatccgc gcgaggacat aggagttgac
      481 gaactcgccc gcccagaaag ccgccatcga tgcgagcacg atgcgccacg cggcgccgaa
      541 cacgctttca taggtttgct ggcagaccgc gccggggccc accgcctcgc ccgatttcag
      601 cgccagcgtc tcggtcccgc tgcaccacca atcctgggcc ggcggcatcg cgagcacgat
      661 ccagctcatc accgccatga aaaggagagc cgcaaaaccc gtccagatca cgcgccgcgc
      721 gcgcgcatag ccatatacct cggtcagcac gtcgccgatg acatagctga tcggaaagaa
      781 gaggatgccc gcgccgaagg tcagcccgcc gaccatcgac agcttcgacg cgccgatgat
      841 gttcgacagc agcagcacgg ccacgaacgc ggccatgacg aggtcgtaat agcgaaaatg
      901 ccggatgctt ccggcgctca cgggtgcggg tttggcgtgg gtcgcgtcgg aatcgatcat
      961 tggccgctgc ttaacctgct ttgcggcgga cgcaaaccgc gctattcgcg gcgccgcaca
     1021 gccgtgccgc gcgcccgtag ctcagctgga tagagcaccc gccttctaag cgggtggtcg
     1081 caggttcgaa tcctgccggg cgcgccaaca cttccgcaga aaacatgggt ttattgcaat
     1141 cgtgccgtgc cattattggc acgtatagtt ccatttcatg ccgtcattgc agcaagcgat
     1201 cagtcgcatg cgagcgatgt atcgaggcct cccccgaccc gtaacgggcc ggtgccgacg
     1261 cgtcatttcg aaggagcgag aaaaagccag ccaccaccga aatcgatcac agccgcacgg
     1321 gcgatccaga aggtggcacc gagcgcgccc tggatgggac cgggagccgg agcgccttcg
     1381 gcagctgacg atcgggacaa ataggccccc gtgtcccacg ccggcggctg gaatccggga
     1441 aaatgccgac cgccgatttc gatcgatagg ggggccaatg gcttgtacac cgggacggtc
     1501 gcgccttttg gtccgcctgc cgtgcctgtt tgctcgccaa agaccagacc tgcggcttgt
     1561 gacgacggca ggtcgagata acctgccgac acagtgttca cgatcaggtt ggcggagtgc
     1621 cccgaaagcc ggacattgac ggtataggcg gcctcctttt cgttccagat catgggcaac
     1681 gcggtatagc cggcttcggt cagacgtgcg cgcgcgctgg cggtgtcgtt ggcttcttgc
     1741 ggaaagccga tccgcttgcc gccgaagtcg acaaatgccc gcgatgatcg aagccacttg
     1801 attcccagca tgccagcctt gccgctccga agaatatcgg ccttgttgcc aggtagcgca
     1861 tggacaaatg ccagagcggg gcttgtgatg ttcggtccga tcgtcagcgt gccgagccgt
     1921 acgcgggacg cccccgccga agcgagatgt ccgctgcggt cgatgccgta ttggccggca
     1981 taaatacgat cgtgatagcc caatgcatct gcggcttccg gcgtcagctc gatcaggccg
     2041 gcggcgccgg aatgcacggc cagcgcaaca gcggtgtcgt cgatacgggc atcgacgacg
     2101 gggcgaatgc cgaaatcctc cttcatgacg aagggcacat aggcaatccg agcctgtgcg
     2161 gagctggtcg cgataatcag gctgaaaaat atcgataggg ccaatcgcaa gtcactatcc
     2221 ttgacgtttc gataaacccg actgggggcc cggccgaggc acttcgaaga tatatatctg
     2281 tgcgttcaag cgatagcggg gtgccgcgtt tctgccaagc atttgaatat gatgcagtcg
     2341 tcatggagcc agcaaccggc cgccttggac tcaggcgccg tgatagccgg tcagcgcgtc
     2401 ttcgaaatcc tcgatcggcc ccacattttc tcgtgccggc gcgccagatg ctgcgggaaa
     2461 caccgcatcg attcgttgac gcaatgtgtc atcgagatcc cattgcttcg aaacgcgtgc
     2521 gcgctttgcg acagtgagtt ccgaacatat cgcgtccttg atgcggtcga gcccggcggc
     2581 gcttaggacg cccgcgttca gcagctcttc gcatagctgg atcaggccga ccgccgttgc
     2641 gtgcgcacgc agaccaatca ggtccagcgt ctggtggcgc tcgcgagcgg catcggtttt
     2701 tgcggaattg ttcatcgata ttctcctcat ccctccattg atcgaggcgg gtcggcctca
     2761 cggtcagctg gtagtcgtca ggccgggagc agcgccttga gcggggtttc ggggtcggcg
     2821 agttgctcgg gggtcgcgcg aagcccggcg gtaacgatca tgcggccctg gacataatcc
     2881 ctggtggcgt tgacgcagtc gatcgcgatc accttgcccg ccttgaggta gacgaccgag
     2941 aagctgcggt tcgccgggtc gccgcgcagc accgcctgat cgtgtccggt cgagaggcca
     3001 gcggtctgga gcttgaggtc atactggttc gaccagaacc acgggatcgc atgatagggc
     3061 gcttcgtcgc cggcgatgcc cttggcgacg acattcgcct ggtcgttggc gttctgcacc
     3121 gattcgagcc ggatctcggc gccctcggca aagtcgttcg tatgcgatgc gcagtcgccg
     3181 atcgcataga tgtcggcgag gctcgtcttg cagagcgggt cgacgcgcac cccgttggcg
     3241 caatcggcac ccgccgccgt gagcggctcg accgcgggaa cgatgccgat gccgacgatg
     3301 acgaggtcgg cgggaatgac ctcgccgtcc gacagccgca ccccggtgac atgatcgtcg
     3361 ccctcgatcg catcgacgca cacgccgagc cgcaggtcga cgccgtgctc gcgatgttcc
     3421 ttttcataga agcgcgacag ctcgaccccc gcgacgcgcg cgagcacgcg gtcgagcgct
     3481 tccagcagca cgaccttctt gcccgccttg gtgagcaccg ccgccgcctc gagcccgata
     3541 tagccgcccc cgatcacgac gatctgctcc gcgctctccg acgccgcctt catcgcatcg
     3601 gcatcggcgc gcgtgcgcac gccctgcacc ccgggcagat cgccgccggg gatcggcagc
     3661 atgcgcgggg ccccgccggt cgcccagacg agcttgccgt aaccgatcgt ctcgccgtcc
     3721 ccggtggtga cgctgtggcc ggccggatcg accgacacga cacgccgacc gagcagcatc
     3781 gtcacctcgc gctcgtccca atatcgggcg gggcggagct ggatgcgctc gaattccttc
     3841 tcgcccgcga aatattcctt cgacaagggc gggcgttcat agggcagctc gggctcttcg
     3901 ccgatgatcg cgatgctgcc ttcgaacttc tgcgtccgca gcatcaccgc cacctgcgca
     3961 cccccatgcc ccgcacccac tatcaccacg tcgaattgca tcgcgtcctt ctcccatccg
     4021 aggcgagggc aagttcgggc ttgcctatgc atattagata aataatcagg tgcgacgctc
     4081 gcgcaagcat catcggctgc ggcgctgtcg aagagctgtt atccgtcata cggatgcggc
     4141 aaggtgcgga gaggtggaaa tgcaggatga cacgaatttt accggccggc atgtcctcgt
     4201 ggtcggcgga tcaagcggta tcggaaacgg gattgcccat ggtttccgga ttcgcggcgc
     4261 cgaggtgcat gtctggggga cgcgagcggc cgcttcggat tatgatcccg gcgagggttc
     4321 cgatctcacc gggctcggtt attcctgcgt cgatgtcggt gattccgccg cgatagaagc
     4381 ggcggcggct ccgttcgaaa cgctcgacgc gctcatcctt tgccagggaa ccgttgtcta
     4441 tggccgcggc gagttcgagc gggacggctg ggaccgggtc atggccgtca atctcgacag
     4501 cctcatgcat atcgcgcgca aattccgggg ccagcttgcg caagcgcgcg gatccatcgt
     4561 catcgtgagt tcggtgtcgg gcttgcgggg caatatcgga aaccccgcct atgcggcctc
     4621 taaagcgggg gcgataagcc ttaccaggag tctcggtcag gcgttcgctt cggacggcat
     4681 tcgcgtcaac ggccttgcgc cgggtctcgt cgacaccaag ctgaccaagg cgaccacctc
     4741 gcatcccgaa cggctggaga agagcctggc ttcgattccc cagcgccgga tgggaacgcc
     4801 cgacgacatg gcgggcgccg ccatcttcct cgcatccgcg ctcgcatcct atgtgaccgg
     4861 ccacaccctt gtcgtcgatg gcgggctgac gctctgaacc ctccagccct gttttagatc
     4921 gccagcacgg caagggcgtc acccgcggcg cagcgggggg ctcgcgccgg gcggcgcacc
     4981 aatatcgtcg cttggccgag cagattctgc gcggatgctt cctgccgatc gagaaggagc
     5041 gctgcgccgt cgacgatatc gccgatcaga aaggcctcgc gctctccctg cgcctcgacc
     5101 tcccgagccg cgcgtaaatg cgtccatgcg agcgcggatc gcggctcgcc gccgcccaga
     5161 cccgtcaata gcggcgcgag aaaaagcctc gcgacggtga gggcggcggt ggggttgccg
     5221 ggcaagccca ggacatgcgc ctcgccgacc ttgccgtacc agctcggctt gcccggcttg
     5281 atcgcgacat cggcgaagag gagccgaagc ccgaccggga ccagcgccga gcggccgaaa
     5341 tcgcgatcgc ctcgcgaggc gccgccgatg atgaccgtca catcatcttc cttggagaag
     5401 gcatcgcgaa tggcacgcgg atcgtcgccg atacgcgtct cgcgcaccac gcacccgccc
     5461 cattggccgc tgagaagcgc gatcgccgcc gtcagactgt cggggatgcg gtccgcacgg
     5521 ttggccgcct cgccgggcgg gacgagctcg tcgcccgtcg cgatgatgtg aaccctgggc
     5581 cgtcgccaga cggtgacgtg cgaaacatcg ccggccgccg ccgcgacgag cgcgacgggg
     5641 gtcagttcgc ggcccttgtt cagcagcgtc tgaccggcca cgaaatcgga tccgcgccgt
     5701 cgcacatggg gcttgctgcc gggatcgccc cgtatctcga ccatcccgcc gatcttcgtg
     5761 caatattcga tcatgacgac ccgatccgcg ccatcgggca ggggtgcgcc ggtcatcaca
     5821 cgcatcgtag aacccggggt aacagccgtc agcgcgacgt cgccggcgac gatttcgccg
     5881 agcgaggcga agcgccgcag cccggccgcc agatcgccat cgcgcagcgc atagccatcc
     5941 atcgccgcgc aatcccaccg ggggccgtcg atccgcgcca ccaccggttc ggccaagcgg
     6001 cgaccggcgg ccgcgccgag cgcaacggat tcgtgtccga gcgcatttgc cgcgttcgac
     6061 aggatcgcga cggcatcgtc gaagaccagc gatgcttggc acatggccgc gcccggttcg
     6121 gccatcccga tgcggcggct cacgatccgc tcgcgcgcgg tcgcctacgc atagggcggg
     6181 gcctcttcga actcggcaaa ccaaggatag tcgttcgcga cgctcgcggc gaaggccggc
     6241 gggcgctttt ccagaaaact cgacacgccc tcggccgcat cggggccgcg gccgcgcgcg
     6301 aagatcacgc ggctgtccca gcgatgcgcg ctcatcgggt gcggtgcgcc catcatgcgc
     6361 catagcatgt gacgggtgag cgcgaccgaa accggggcgc tgtcggcggt catctcgcgc
     6421 gccctggcga tcgccgcatc gagcagttcg ccgggttgat gcaccgcttg gacgaggccc
     6481 ttgtccaagg cctcggcggc cgaaatcagg cgccccgaat agcagaggtc gaccgcgttc
     6541 gcgatgccga cgatgcgcgg cagaaaccag ctcgacgcgg cttcggggac gagcccgcgc
     6601 cgcgcgaaaa cgaaaccgaa acgggcattg tcgctcgcga gccgcgcgtc catcggcaac
     6661 gtcatcgtca cgccgacgcc gaccgcggca ccgttgatcg cgccgatcac cggctttttc
     6721 gcatcgaaga tccgcaacga tacgtggccg ccgccatcgc ggacgaggtc atgcgaccag
     6781 tcgatcgtgc cgtcggcggc gatcgggccg ccggatgctc caccttgctt ttcataatcg
     6841 aaagtcgaag cgcccgcgct gagatccgcg cccgcgcaat aggccctgtc gccatgaccg
     6901 gtgaagatca ccgcgcgcac gccgtcgtcg gcgtcgcatt cgtcgatcgc ggcgatgatc
     6961 tcgtgcatca tctcgaccgt gaaggcgttc atccgttcgg ggcggtgcag cgtcaccacc
     7021 gcgatgcctt catgcttgtc gaggcgaatc tgatcgtagg ccattgtctc cctttccttg
     7081 aacccggtgc cggcgcgcga ctcccatatc tatgcctatt aaatattctt tgggccgctt
     7141 ggcgggtctt ggcaagggtg gcaagttcct ccgttagttg atcggcggca ccgccggtca
     7201 gcccggtcgc cggtcgcgca ggatgatatc ggcggcgcgc caagccatcg ccatcgtcgg
     7261 gccgttggtg ttgcccgacg ggatcaccgg catcaccgac gtatcgatga tccgcagccc
     7321 ctcgacgccg cggacgcgca gcgcgggatc gaccaccgag ccgtcgtcct ttcccatgcg
     7381 gcagctgccg acggcgtgat agccgcaggt cccgaagcgg tcataggcgt cgaggatttc
     7441 ggcgtcgctg ccgaacgctg gcccgggcat cgtttcctcc gcgatcatgc tcgccaacgg
     7501 cgcccgcttg gcatagtcgc gcatcacgcg caccgcgccg accatcgcgg cgcggtcctc
     7561 gtcggtcgag cgatagttgg gcttgaaact cgctgccacg tccgggtcgc gcgtcgcgat
     7621 gtgaatgctg ccgcgcgagg tcgggcgcag gggatagatg accgcgttca tgcccggatg
     7681 tttttcgagc tgcgttcgct gcgtcgccat gtcgaagctg aacggcgcga tgagcatctg
     7741 cgcgtcgggg cgattgagcc ccgggcgcgt cttgaaccag ccgccgatct cgtatgccgc
     7801 ggctgacatc ggcccatcgc cggtcagata atatctgagc gtcgcgagca gcagccgaca
     7861 gccgccgaag acgcgatttt gcgacagcgc ttgcctagtc agcttccact gcatgatgat
     7921 cccgcgatgc tcgatcagcc cttcgccgac ttcgggatta tgatggacgg gcgggatgcc
     7981 gagccgggca agaagattgg aatcgccgac gcccgagcgt tcgaggatag ccgggctcgc
     8041 catggcgccg ccgcacaata tcacttcgtg cgccgcgccg atcgtctcgc gcacgccatt
     8101 gtggaccacc tcgaccccga cggcgcgcgc gccatcgaac cggacgcggt cgacggtgcg
     8161 ttcggtcaat atggtgaggt tgggcctttt ctcggcgggt ctgaggaaag cctgcgccgc
     8221 gctttggcgc ttgcctttga atatcgtgcg cggcgcatag ccgatcgcga cctcgtcgtc
     8281 gggcgcgttg acgtcgggct tgttcgccca gcccatcgct tcgcccgcgg cgacctgggc
     8341 ttcgctgatc ggcaggcgct gcgtcggcat cgaaattttg agcggtccct tgtcgccgcg
     8401 cgtttccgcg ccgcccagct cgtgattctc gagcgcgcgg taggcggcgc cgatatgcgt
     8461 ccagctccag tcgtcgctcg atttctccgc gatcgcgtcg aagtcggcgg gctggccgcg
     8521 cacatacatc atgccgttga ccgacgaaga accgccgagc acgcgcccgc gcacccacgc
     8581 ttccgactgg cccgcggtcg atgcctcggg ctcgctcata tggacccaga gatgctttgg
     8641 atcctgcatg accttcgcga gtcccttggg catcgagatg aagggatgac gatcgcgcgg
     8701 ccccgcctcg atcagcgcga ctctgacgtt cggatcctcc gacaaccgat aggcgagaac
     8761 gcagcccgcg gcgccggcgc cgcaaatgat atagtcgaaa tccatcctca tatccctcga
     8821 ttttggccgc ttcggcggcg ctctcgcctg tttcataaaa aactattgtc attaggtaaa
     8881 gcgtcgaata ggctgacgcc aacgacaagg agaggatctg caaatgagcg atgttgcaga
     8941 agtttcacgg ccggcacgca aggtgacgac gctcgatgac ctgacgccga gccagatcga
     9001 ggcgatccgc aagatccccg ccgagaagga cgcggtggtt gtgccgttag gcgcgacccg
     9061 ccccaatgcc atcttcaccg gccgcgaacg cttcgacctc gaacaggaaa aaatcttccg
     9121 ccgctatccg gtgccggtca ccgtgtcggc gctgctcgaa cccggcagcg tcgtggcgaa
     9181 cgacagctac ggtttcccga tgctcgtctc gcgcacccgc gacggcacga tcaaggcgtt
     9241 cgtcaacgcc tgccagcaca agggatcgaa gatcatcgag gattgcgcgg tccacaagca
     9301 ggggcgcatg acctgcccct atcatgcctg gacctatggt atcgacggca agctgatcgg
     9361 cgtgtcgcgg agcgaggcct tcgagggttt cgacaagagc cagcgcggcc ttgccgaact
     9421 cgaggcgcgc gaatggggcg gcatcgtcta tgtccagctc aatcgcgata ttcccgcgga
     9481 ctggtcgcac ttgtcggacc aggtcgccga ggatttcggc gcgctgggca tcccgtcggc
     9541 gcatgtctac gggcgcaaga cattcgacct caaagccaat tggaaggtgg tgctcgaacc
     9601 cttcctcgaa ggctatcacg tccagcggct tcacgccgcg tcgatcggcg acaaatttca
     9661 ggacgcgccc aacatcgtcg acatgttcgg ggcgaatatc cgtcaggtgt cggggcggat
     9721 cggctatgtt cccgaaatgc tcgacgagga tccgttggcg aacgtccaca agctcgtcac
     9781 gcacgcctat acggctttcc ccaactgcgt catcgtgacg agccaatatt ataccagcgt
     9841 gatgctgctc atgccgcgcg acgccgatcg cacgacggtc gaatatttca tgctgacacc
     9901 cgaagcggcg gcgaccccga aggccgagga ggtgttcgcg cgctcctacg aacttatcct
     9961 cggcgttttc ggcggcgagg attttcgcgc cgccgagatc agccagcagg ggctgaacgc
    10021 cggggttccc gaggagaccg tctattgcgg gctcgagacc aatatcatcc gctattatga
    10081 ggcgatcgaa gcgcttctgt aatttgaccc ggcaaagacc ggatggagag gactgaatgg
    10141 cgcagacttt gtccggcgcg ttggcctggt gggcgcggat gcggcccgag cagcccgcgg
    10201 tagtgctcgg cggcgaccgg ctgggttacg gcgattacaa ggcatggtcc gatcgggtcg
    10261 ccgcgatgct ggaggccgat gggctgcggc cgggcgaccg cgtcgcgatc tgctcgacga
    10321 acagcctcgc ctattgcgcg ttgctcatgg gtgtcatccg tgccgggggg attgccaatc
    10381 cggtcaattt tcgtttcacg ccgcgcgaaa tccgcgaact gtgtgaaacg agcgagccgc
    10441 gcttcacctt cgccgcgcct gatttcgagg cgaatgtcat cgccgcgggc ctgaccccgc
    10501 gcgccatggc cgagatcgaa gcgttgcgcg acggcgggcc ggcgacggtc gggcacgatc
    10561 ccgaccccga cgccccggtc gtgatcatcg caacgagcgg ctcgacggca aagcccaagg
    10621 gcgtcgtctt caccaaccgt tcgatgaccg cctatgccgc caactgggcg gtcgaggaaa
    10681 cggcctcctc gcccggtgcg cgtgtgatca gcctcgcgcc gctcaacacg tcggccggtt
    10741 ttgtccagct gatgcattat acggttcagg gctgcacgat cttcatggag ccggtcttcg
    10801 cgcccgacgc gatcctgcgg ttgctgcagg acgaaaagat caactgcttc ggcgcggttc
    10861 ccaccttctt cgaggcgatc gcgcgctgtc ccggcttcgc cgatgccgac ctgtcgtcga
    10921 tccgtctcgc gacggcgggc ggcgcgcgcg tcagccgcca gttgctcgac gcgtacaagg
    10981 cgaagggcgt gacgatccgc cagatttacg gtcagaccga aatcggcggc aacgcgacga
    11041 tgatgcccga acatctcgcg atggacgagc ccgagaaatg cggctgggga tgcgtgttca
    11101 tggacctgcg cgtcgtccgc cccgacggca gcgactgcga tcccggcgag cccggtgaaa
    11161 tcgtcatgcg ctcgcccggg atgatgcagg gctattggcg caatcccgag gagaccgcga
    11221 aagtcatcag agacggctgg ctctggtcgg gcgacatcgg gacgctcgac gagcgcggcc
    11281 ttttgacctt cgtcgaccgg atgaaggatc tgatcatctc gggcgggctc aatatctcgg
    11341 cggcggaggt cgagcgcgtg gtgtgcgaat tcccgggcgt ggtcgaagcg ctcgcgatcg
    11401 ccgctcccga cgagaaattc ggcgagacgc ccttcgtcgt ttaccacggc gcggccgagg
    11461 tcgacgtccg ggcgctgatc gaccactgca acgtcaatct ctccaattac aaggtgccgc
    11521 gctacgtcgc cttttcggtc gacccgttgc cgcggctcgc caccggcaaa ttgtccaaac
    11581 ccgcggtgcg cgcgctctac gccggcgcgc acgagacgat ggagcgcgtg cgatgaggag
    11641 cctgccatga ccactgctgt gctcgccgcc gcgccggtgc cgccacatgt tccggcgcat
    11701 cttgtcttcg atttcgacat ctatgccgat ccgcgcatcg gcgaggatgt gcagggcact
    11761 tatgccgccg cgttgtccga cgcgcccgac atcttctgga cgcggctcaa cggcgggcac
    11821 tggatcgtca agagcttcga cgcgatcagc caggtcgtgc tcgaccccga gcatttctcg
    11881 gtgcgcgaga tgcagattcc gcgcgtcgag aacccgcctt tcttcattcc gctgagcctc
    11941 gatccgcccg aaaaccttcc ctatcgccgg gcgatgatgc cgatgttcgg acccgccgcg
    12001 atcaaggcgc tcgaaccgcg cattcgcgaa tgggcggtcg agctggtcga tgcggtggcg
    12061 acgaagggcg cgtgcgattt ccaggccgat ctggcgaagc tgttcccggt cagcgtcttc
    12121 atggagctca tggggatgga cctctcgcgg ctccgggatt ttcgcgcgct tgccgaggcg
    12181 tttttctcgg cccagaatga cgaggccgaa atgggccgcc tcggcgccgc gatccttggc
    12241 gagatgaagg cgctgatcga ggcgaagaag gcggcgcccg acgacaagtt gatgtcgcat
    12301 ttcatcaccg tcgatatcga cggccgccgg atgagcgacg acgaaattct cgcgatgagt
    12361 ttcgtgcttt tccttggcgg catggacacg gtgaccaacg tcaccggctt cgcgttccag
    12421 caattggcgc aaatgcccga ggtgcaggcc cggcttgccg ccgacccgtc gctgatcccc
    12481 gccttcgccg acgaggcggt gcggctctac ggcgtcgtca acacgccgcg gctggtggtc
    12541 cgcgaccagg agatcggcga ggcaaagttt cgcgagggcg aaatgctgct caacatcctc
    12601 tgcctcggca gccgcgatcc gacaaaattc gaagcgccga acatgttcga cctcgatcgc
    12661 cggaagaccg cgcacctgac cttctcgtcg gggccgcatc tgtgcgtcgg ccatgtcctc
    12721 gggcgcgccg agctgcgtat cctgaccgag gaatgggtga agcgcattcc gcggttccgg
    12781 gcggttcccg gcgaaaagca cggctttcgc atcgggacgg tgatggcgct cgaatcgctg
    12841 ccgatcgaat gggacgtcgc cggctgatct agcggatctg gtcgagcggc ttgccgatcg
    12901 tttcggtcgc cagccgctgg cggacaccga ccaggaacat acgcagcgat tcgacgaaca
    12961 attcctcgtc gccgaaggga ttgtcggcca ccgccttggc aaagttcgga tatttggccg
    13021 cgtcgaccga cgggatcggc gggatgccgc gcacctgtgc cgacgcctcg aacagcgcgg
    13081 tgccgaaggt atatttttcg atcgcgtcga tcaccgtcag gtggatcgag gcgggatagg
    13141 gctcctcggc tacggcgcgc tcataggccg cgaggagaag gtggcgcgga aaatagcgga
    13201 gcatcagcgg cgccgcgtta ggatgcttga gcaaggcgcg gcgcgtcacg acgcagcgcg
    13261 cgataatctg ttcctcgaag gacagggcgg ggtcgttcac cgtcgggatg cgcacgaaca
    13321 tcatgcgcgc gacctcctga agcagctccg acttgtcctt gaaatgatga tagagcgaag
    13381 gcggccgcac gccgagcttg cgcccgacga ggctgaggct caactcttcg agcccgactt
    13441 cgtcgatcac ttccagcgcg gcctccgccg cattggagcg cgatatcagc ggtttggacg
    13501 gtcttgccaa atgtcgatct ctccttgccc gtcgctcttg gcagcgcgga acggcgagga
    13561 ccagcgcaaa aatcggccgg gtccgtgcgg cgtgttcaat ctattgacgt tagataagcg
    13621 cggagccatt gtcgagagcg aggagagcca tgcgatgacc aaccgcctgc aacgcaaaac
    13681 ttttgtatcg cccgacgggg tcaggctggt cggcgatcac ggcggtccga tcgacgcacc
    13741 gtgcgcggtg ctgatgcatg gcggtgggca gacgcggcac agctggagcc gcgcggtcga
    13801 gcggctgctc gacgccggac tgcaggtgat caatttcgac gcgcgcgggc atggcgacag
    13861 cgactggtcg ccgggcgggg cctatgcgct gaccgatcgc gcggccgacc ttgccgcggt
    13921 gacggcggat cttcacgtcc ccttcatcct gatcggcgcc tcgctcggcg gtgcaacctc
    13981 gatcgaggcg gtccacgagg gattgcggcc cgcgggcgtc gtcctcgtcg atatcgtgcc
    14041 cgcgcccgac ccgcgcggta tcgaacgcat tgtcggcttc atgcacgggc atcagggtgg
    14101 tttcgcgagc gtggacgagg ccgcggcggc ggtcgcggcc tataatccgc agcgcgaacg
    14161 gccgcgcgac acgcgcgggc tgatgaagaa tttgcgtccg cgcgatgacg ggcgcctcta
    14221 ttggcattgg gatccgcgca tcctcgagac cgcgccggcg atccatcatg ccgccgtcgc
    14281 gcgcgcggcc gacacgctcg cggccctcac cgacattccg gttctgctcg tgcgcggcct
    14341 gtcgagcgac gtcgtgagcg aggcgggggt cgatgccttc cggcggcaga tgccgcggct
    14401 tgaaatcctc gatgtgctcg gcgccggaca tatggtcgcg ggagaccgca acgatgcgtt
    14461 caatgacggc gtgatcgcct tcgccaagcg gcttttggtg tccgaggccc tgtcgtcatc
    14521 ttcatctatc gatttgaaat aaatataaaa aataccgtct ccgcgaaaac cttatggcgt
    14581 taaatttgcg attgacttta aatctaatga ctgtaggtta atcctcaagg ccgcagataa
    14641 tggcctgatc tttgggagga gacggtcatg gatcgcaacg ttacgcgtcc ggcaattcgc
    14701 tacgcttgcc tggcatcggt catagccctc gcagcggtcg cgccgcccgc cttcgcccaa
    14761 accgatccgg ctccgccgcc gcccgccgac gaggctgcgg ccggcagcat ggccgatagc
    14821 gaaatcatcg tcaccgcccg ccgccgcgac gagcgcctga tcgacgcgcc cgtggcgatt
    14881 accgcgctcg gcggcgcggc gctcgacaat tatgcggtca ccgaattcaa cgatattgcg
    14941 cagctcgtgc cgacgatggt cgcaggcaag gcggcgtcgg gttcgtcggc gagcatcttc
    15001 ctgcgcgggg tcggctcgac cgcgctcagc gccggtttcg accagtcggt gtccttcgtc
    15061 gtcgacggca tgccgatgag ccgcggccgc gagatcggcc tcagccaata tgacgtccag
    15121 cgcgtcgagg tgctcaaggg accgcaggcg ctgttcttcg gcaagaatgc caccgcgggc
    15181 ctgatcgcgg tcaccaccaa cagcccgcgc gatgtgttcg aggcggggct caagatcggc
    15241 tatggcttcg aggcgcagga gaaatatggc gagggctata tttcgggtcc gctcgccgac
    15301 gggctgcgcg cgcgcctggc gttccgtatt tcggacagtg agggcgcctt caccaacacg
    15361 gccgcggaaa cctacatcga tccgttcggc ctcgaacgcc accgcaacag caagaaccgc
    15421 ggctatgggc ggacctacag cggacgcgcg acgatcgaat gggacgcggc cgacaatctg
    15481 acctttcagg tcaagaccgg ctacaccgac cagaaggatg gcggcccgac cgacatcatc
    15541 gaacgcatct gcggtggcgg tcgcaccgtg cccttcgccg cgaatggcat cccgccgagc
    15601 cccaatgccg attgcgtcat cgacggccgc tcggacagtt cgacgatccc gatcgaggtg
    15661 gcgcagctcg actaccgcta tgctggcgac ggccgcatgt attcgcggct gaaatcgggc
    15721 ttcggcatcc tgaccgcgac gctgaccagc gacgtgttcg acgtcacctc gatcacctcc
    15781 tattatcgct tcaagcagaa cgacctcaac aatgtgtcgg gcgaagccta tccggcgact
    15841 ttctcgcagc tcgccgatta tcgccagacg tcggaggaat tgcgcttcca gtcgaaatgg
    15901 gacgggccga tcaacgcgct gttcgggctg ttcgtctcga gcaacaagtt catcttcaac
    15961 accgacgcct atatttttcc gctgccggcc gttgccgcag gcacgaccta tgtgaccttc
    16021 aagcgcaaca acggcttttc gggcagttcg atgtcggcct tcggcgaagt gaccgccaat
    16081 ctgaccgaca gtctcgaact cgcggggggt gcgcgctgga gttacgaatc gcgcgacagc
    16141 ttccaggagt cgctggccgc caatccggcc ttcgccgccg cctttccggg cggactgcgc
    16201 ctcgacgatc gctatcgcga aaccgacgtg tcgccgcagg tgacgctgcg ctacaagccg
    16261 tcgcgcgacc tgaccttcta tgcggcctat aaggaaggct tcaagtcggg cgggttcaat
    16321 at
//
LOCUS       DATMSU010000534         4162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_150657,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000534 DATMSU010000000
VERSION     DATMSU010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150657"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4162
                     /locus_tag="VN034_14380"
     CDS             <1..>4162
                     /locus_tag="VN034_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HWV61846.1"
                     /translation="RIVPVKCRRPLPPGREMALVWDARISQAGVPGRTAGRDQRFDYD
                     VRPAFTAKMSCSRVNPQAGCNPIKDVVLSFASPVRRESVLAATLSTSDGKTLTPKIDD
                     DDKNDAWLTTVRFAGPLPQNVDATLVLPANVTDQSGRNLQNQSNFPLKFHIDRAPPLV
                     KFAADFGILEAGEGGVLPVTVRGVEASLVQSNLKMPVAALRVGDDDAAIARWLRRVDD
                     ADDTDYREEKNAAGKDVTVNYTGTKSVFVGAPAGGERRNLQLTPPGGGKAFEVVGIPL
                     AEKGFHVVEIASPELGAALLGRRETRYVATAALVTNMAVHFKWGREGSLAWVTALDTG
                     LPVGGAEIRVTDSCTGRLLARGTADKAGRLAFPSALPEPETYSSCEENPDMAKSEGHA
                     LMVSARSEGDFSFTLTDWGNGIRPYDFDLPYGWSERADILHTVFDRALVKAGETVHMK
                     HILRRPIGLGFRTPEPLAGKLRFVHRGSDTEFEMPFAIPATGSGETVWNVPASAPMGD
                     YELVFVTKDKDGEDKTIWSNQSVRVDEYRLPTMKATITGPKTALVRPTAVPLNLFVGY
                     LSGGPAPNIPVELRTNFRATWSPPEDYRDWDFDGQPVKEGTVQLDDSGDTPSADLPLA
                     RSVPLTLDANGTATTSVTVDQPITEPTLMAAEMDYEDANGETLTSSRRITLYPSAVRL
                     GLKTDGWLMRDNDLRLNFIALDLEGKPIRGQRVDVALYNREIITARRRLIGGFYAYDN
                     QMRTTRLGATCSATTDRLGRASCAMAPGISGEVTVVATTQDADGNEARAVRSVWLAGD
                     DDWWFGGDNGDRMDVIAEQPRYDAGDTAKFQVRMPFREATALVTVEREGVLSSFVVPL
                     KGTNPVVKVKLPATYAPDVYVSVMAVRGRVTGGESWFRKLKRAAGFKIENSEGAPPTA
                     LVDLAKPAYRMGIARIKVGWEGHKLGVKVKADKEKYSVRETAKVAIEVETPGGKAPKN
                     ADVAFVAVDEALLQLAPNESWEILSAMMGERTLDVLTSTAQMQVVGKRHYGRKALEPG
                     GGGGGDLSGLTREDFRPVLLWKGNVPLDAKGRARVDVPLSDNLSGFRLVAIATDGSQY
                     FGTGETTVRTVQDLGVFAGMPELVRTGDTYDARFTLRNGTDKAMEVTATPTLSPAIAT
                     APPLTVTIPAGGAVPISWSMTAPDRTGPIEWTVEAASKDGKARDRLVFEQTIEPAVPI
                     ETWAASLFRVGPATTLPIAIPAGALPGGYVDVALAGTLAPPLTGVRDYMSAYPYTCFE
                     QQTSRAVALGDVGRWQALAGAMPTYLDDDGLLRYWPNERLEGSIELTAYVMSVTAANG
                     FAIPEASREKMVKALQAVVEGRLTRKGYGPWDIRPVRIAALAALARNKASSASLVAAI
                     DVAPVDMAT"
BASE COUNT          711 a         1499 c         1347 g          605 t
ORIGIN      
        1 ccgcatcgtc cccgtcaaat gccgccggcc attgccgccg gggcgcgaga tggcgctcgt
       61 ctgggacgcg cgcatatcgc aagctggcgt ccccggccgc accgcgggcc gcgaccagcg
      121 cttcgattat gacgtccgcc ccgccttcac cgccaaaatg tcgtgcagcc gcgtcaatcc
      181 gcaggcgggc tgcaacccga tcaaggatgt cgtgctgagc ttcgcctcgc ccgtgcggcg
      241 tgagagcgtt ctcgccgcga cgctgagcac ttccgacggc aagacgctca cgcccaagat
      301 cgacgacgac gacaagaatg acgcctggct caccaccgtg cgcttcgccg gcccgctgcc
      361 gcagaatgtc gacgcgacgc tcgtccttcc cgcgaacgtc accgaccaga gcggccgaaa
      421 cctgcagaac cagtcgaact ttccgctgaa attccacatc gaccgcgccc cgcctttggt
      481 caaattcgct gccgacttcg gcatcctcga ggcgggcgaa ggcggcgtgc tgcccgtcac
      541 cgtgcgcggg gtcgaagcca gcctcgtcca gtcgaacctc aaaatgcccg tggccgcgct
      601 ccgcgtcggc gacgacgatg ccgcgatcgc gcgctggctt cgccgcgtcg acgacgccga
      661 cgacaccgac tatcgcgagg aaaagaatgc cgccggcaag gacgtcaccg tcaactacac
      721 cggcaccaaa tcggtgttcg tcggcgcccc ggcgggcggc gaacgccgca acctgcagct
      781 gaccccgccc ggcggcggca aggcgttcga ggttgtcggc atcccgctcg ccgaaaaggg
      841 tttccacgtc gtcgagatcg cgagtcccga gttgggcgcc gcgctgctcg gccgccgcga
      901 gacgcgctat gtcgcgaccg ccgcgctcgt caccaatatg gcggtgcatt tcaaatgggg
      961 ccgcgagggt tcgctcgcct gggtcaccgc gctcgacacc ggccttcccg tcgggggcgc
     1021 cgaaatccgc gtcaccgaca gctgcaccgg ccgcctgctc gcgcgcggca ccgccgacaa
     1081 ggcgggccga ctcgccttcc cgtcggcgct gcccgagccc gaaacctatt cgagctgcga
     1141 ggaaaatccc gacatggcca aaagcgaggg ccatgcgctg atggtcagcg cgcgctcgga
     1201 gggcgatttc agcttcacgc tcaccgactg gggcaatggc atccgcccct atgacttcga
     1261 cctcccctat ggctggtcgg agcgtgcgga catcctccac accgtcttcg accgcgcgct
     1321 cgtcaaggcg ggcgagacgg tccatatgaa gcatatcctg cgccgcccga tcgggctcgg
     1381 cttccgcacc cccgaaccgc tcgcgggcaa gcttcgcttc gttcaccgcg gctcggacac
     1441 cgaattcgag atgcccttcg cgattcccgc caccggcagc ggcgaaacgg tctggaacgt
     1501 ccccgcctca gcgccgatgg gcgattacga actcgtcttc gttaccaagg acaaggatgg
     1561 cgaagacaag acgatctggt cgaaccagtc ggtgcgcgtc gacgaatatc gcctgccgac
     1621 gatgaaggcg acgatcaccg ggccgaagac ggccctcgtc cgcccgaccg cggtgccgct
     1681 caacctcttc gtcggctatc tctcgggcgg ccccgcgccc aatatcccgg tcgagctgcg
     1741 caccaatttc cgcgccacct ggtcgccgcc cgaagattat cgcgactggg atttcgacgg
     1801 ccagccggtc aaggaaggca ccgtccagct cgacgacagc ggcgatacgc cgtcggccga
     1861 cctgccgctc gcgcgctcgg tcccgctcac gctcgatgcc aatggcaccg cgacgaccag
     1921 cgtcacggtc gaccagccga tcaccgagcc gacgctgatg gccgccgaaa tggattatga
     1981 ggatgcgaat ggcgagacgc tgacctcgag ccgccgcatc accctctacc cgtcggcggt
     2041 ccggctcggc ctcaagaccg acggctggct gatgcgcgac aacgaccttc gcctcaattt
     2101 catcgcgctc gatctcgagg gcaagccgat ccgcggccag cgcgtcgacg tcgcgctata
     2161 caaccgcgag atcatcaccg cgcggcgccg gctgatcggc ggcttctatg cctatgacaa
     2221 ccagatgcgc acgacgcgcc tcggcgcgac gtgcagcgcg actaccgacc ggctcggccg
     2281 cgcaagctgc gcgatggcgc ccggcatctc gggcgaggtc accgtcgtcg caaccaccca
     2341 ggatgccgac ggcaacgagg cgcgcgcggt gcgctcggtc tggctcgcgg gcgacgacga
     2401 ctggtggttc ggcggcgaca atggcgaccg catggacgtg atcgccgaac agccgcgtta
     2461 cgacgcgggc gacaccgcga aattccaggt ccgcatgccg ttccgcgaag cgaccgcgct
     2521 cgtcaccgtc gagcgcgaag gcgtgctgtc gagcttcgtc gtgccgctga agggcaccaa
     2581 cccggtcgtc aaggtcaagc tgcccgcgac ctacgccccc gacgtctatg tctcggtcat
     2641 ggcggtgcgc gggcgcgtca ccggcggcga aagctggttc cgcaagctga agcgcgcggc
     2701 cggcttcaaa atcgagaata gcgaaggcgc gccgccgacc gccttggtcg acctcgccaa
     2761 gcccgcctac cgcatgggca tcgcgcggat caaggtcggc tgggaaggcc ataagctcgg
     2821 cgtcaaggtg aaggccgaca aggagaaata ttcggtccgc gaaaccgcga aagtcgcgat
     2881 cgaggtcgag accccgggcg gcaaggcgcc gaaaaacgcc gacgtcgctt tcgtcgcggt
     2941 cgacgaggcg ttgctccagc tcgcgccgaa cgaaagctgg gaaatcctct cggcgatgat
     3001 gggcgaacgc acgctcgacg tgctcacctc gaccgcgcag atgcaggtgg tcggcaagcg
     3061 ccattacggc cgcaaggcgc tcgaacccgg cggtggcggc ggcggcgacc tttcggggct
     3121 gacgcgcgag gatttccgcc ccgtgctgct gtggaagggc aacgtcccgc tcgacgccaa
     3181 gggccgcgcg cgcgtcgacg tgccactcag cgacaatctc tcgggcttcc gcctcgtcgc
     3241 gatcgcgacc gacgggtcgc aatatttcgg caccggcgaa accaccgtcc gcaccgtcca
     3301 ggatctcggc gtcttcgccg gcatgcccga actggtgcgc accggcgaca cgtacgatgc
     3361 gcgcttcacg ctcaggaacg gcaccgacaa ggcgatggag gtcaccgcga ccccgacgct
     3421 gtcgcccgcg atcgcgaccg cgccgccgct caccgtcacc attcccgcgg gcggcgcggt
     3481 gccgatcagc tggtcgatga ccgcgcctga ccggaccggg ccaatcgaat ggaccgtcga
     3541 ggcggcgtca aaggatggca aggcgcgcga ccggctcgtc ttcgaacaga cgatcgaacc
     3601 cgcggtgccg atcgagacct gggccgcgag cctgttccgt gtcggccccg cgacgacgct
     3661 gccgatcgcg atccccgcgg gcgcgcttcc cggcggctat gtcgatgtcg cgctcgcggg
     3721 cacgctcgcg ccgccgctca ccggggtgcg cgactatatg agcgcctatc cctacacctg
     3781 cttcgaacag cagacgtcgc gcgccgtggc cttgggcgac gtcgggcggt ggcaggcact
     3841 cgcgggcgcg atgccgacct atctcgatga cgacgggctg ctccgctatt ggccgaacga
     3901 gcggctcgaa gggtcgatcg agctcaccgc ctatgtgatg agcgtgaccg ccgcgaacgg
     3961 cttcgcgatc cccgaagcct cgagggaaaa gatggtgaag gcgctgcagg cggtcgtcga
     4021 agggcgcctc acgcgcaagg gctatggccc gtgggacatc cgcccggtgc gtatcgccgc
     4081 acttgccgca ctcgcgcgca acaaggcctc gagcgcgagc ctcgtcgcgg cgatcgacgt
     4141 cgcgccggtc gacatggcga cc
//
LOCUS       DATMSU010000535         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_132425,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000535 DATMSU010000000
VERSION     DATMSU010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132425"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VN034_14385"
     CDS             <1..202
                     /locus_tag="VN034_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HWV61847.1"
                     /translation="GWLDKLSGGTATMLVERDGLTGHAENFAAVTLTAPAAPGTIIDV
                     RLTARDGDRMVATQIKEKDIAA"
     gene            199..1125
                     /gene="ftsY"
                     /locus_tag="VN034_14390"
     CDS             199..1125
                     /gene="ftsY"
                     /locus_tag="VN034_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWV61848.1"
                     /translation="MTGQSWSERLLGGFRRTSERLGENLAGLTGKARLDEDDLDRIEE
                     ALITADLGPAMADRIRSRLAERRDIAANGTEELRKVVAEEIAAVLRPVAEPLDIDAFP
                     RPQVILVIGVNGSGKTTTIAKLAHLFQEQDYGVMLVAGDTFRAAAIGQLKVWAERLGV
                     PIMAGPEGGDSAGIVFDAVKQATATGIDVLIVDTAGRLQNKRELMDELAKIKRVLGRL
                     NPAAPHDVVLVLDATTGQNALSQIDVFREVAGVTGLVMTKLDGTARGGVLVSAAERHG
                     LPIHAIGIGETIDDLRPFDADEIAGIIAGNIR"
     gene            1122..1787
                     /locus_tag="VN034_14395"
     CDS             1122..1787
                     /locus_tag="VN034_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HWV61849.1"
                     /translation="MSDVLPTGPEPVAAQPPTTNGWLNYAIDYGPLLIFFVAYKFSSG
                     GEGAFAATTAAIKGTVAFMVAIVVAMAVSKWKLGKISPMLWMSSVLVLGFGALTIWFH
                     DERFIVMKPTIIYAAFAVMLLGGYWYKKPMLKYLLQSALEGLTERGWLLLSRNWGLFF
                     AALGIANHVMYELVQAKQMSFDLWLTIKVWGVTALTFLFTVSQVPVMLKNGLAVAEEA
                     ADS"
     gene            complement(1816..2184)
                     /locus_tag="VN034_14400"
     CDS             complement(1816..2184)
                     /locus_tag="VN034_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61850.1"
                     /translation="MIGYVTLGTNDLARAAAFYDAIAAELETPRMMEYESFIAWGKPD
                     GGAGIGLTKPFDGNAASVGNGVMVALAAKDQEQVQRLYDIALANGGTCEGPPGPRGEG
                     FYAGYFRDPDGNKLNAFIMG"
BASE COUNT          412 a          716 c          734 g          405 t
ORIGIN      
        1 gggctggctc gataagctga gcggcggcac cgccacgatg ctcgtcgagc gcgacggttt
       61 gaccggtcat gccgaaaatt tcgccgccgt gacgctgacc gcgcccgcgg cgccgggcac
      121 catcatcgac gtgcgcctga cggcgcgcga cggcgatcgc atggtcgcta cccaaattaa
      181 ggaaaaggat atcgccgcat gaccggccaa agctggagcg aaaggctgct cggcggcttt
      241 cgccgtacgt ccgaacggct cggcgaaaat ctcgccgggc tgaccggcaa ggcgcggctc
      301 gacgaggatg atctcgaccg gatcgaggaa gcgctcatca ccgccgacct cggccccgcg
      361 atggccgacc gcatccggag ccgcctcgcc gaacgccgcg atatcgccgc gaacggcacc
      421 gaggaattgc gcaaggtcgt cgccgaggaa atcgccgcgg tgctgcgccc ggtcgccgaa
      481 ccgctcgata tcgacgcctt tccgcgcccg caggtcatcc tcgtcatcgg ggtcaacggg
      541 tcgggcaaga cgacgacgat cgccaagctc gcgcatctgt tccaggaaca ggattatggc
      601 gtgatgctcg tcgcgggcga cacctttcgc gccgcagcga tcggccagct caaggtctgg
      661 gccgaacggc tcggcgtgcc gatcatggcg gggcccgagg gcggcgacag cgcgggcatc
      721 gtgttcgacg cggtgaagca ggcgaccgcg accggcatcg acgtgctgat cgtcgacacc
      781 gccgggcggc tgcagaacaa gcgcgagttg atggacgagc ttgccaagat caagcgcgtg
      841 ctcggccggc tcaaccccgc cgcgccgcac gatgtcgtgc tggtgctcga cgcgacgacg
      901 gggcagaatg cgctgtcgca gatcgacgtg ttccgcgagg tcgcgggggt cacggggctg
      961 gtgatgacca agctcgacgg aaccgcgcgc ggcggtgtgc tcgtttcggc ggccgagcgc
     1021 cacgggcttc cgatccatgc gatcggcatc ggcgaaacca tcgacgacct tcgtccgttc
     1081 gatgccgacg agatcgcggg catcattgca ggaaatataa gatgagcgac gtcctcccga
     1141 ccggtcccga acctgtcgcc gcacaaccgc ccaccacgaa tggatggctg aattacgcga
     1201 tcgattacgg gccgctgctg atatttttcg tcgcctataa attctcttcg gggggtgaag
     1261 gcgctttcgc agcaacgaca gcggcgatca agggaaccgt cgccttcatg gtggcgatcg
     1321 tcgtggcgat ggcggtgtca aaatggaagc ttggcaagat ttcgccgatg ctatggatgt
     1381 cgagcgtcct cgtgctcggt tttggcgcgc tgaccatatg gttccacgac gaacgcttca
     1441 tcgtgatgaa gccgacgatc atctatgccg ctttcgccgt catgttgctc ggcggttatt
     1501 ggtacaaaaa gccgatgctc aaatatctgc ttcagtcggc gctcgaaggg ctgaccgagc
     1561 ggggctggct gctcctctcg cgcaattggg gattattctt tgcggcgctc ggcatcgcca
     1621 accatgtgat gtacgaactg gtccaggcga aacagatgag ctttgacctg tggctcacaa
     1681 tcaaggtctg gggcgtcacc gccctcacct tcctcttcac cgtcagccag gtgccggtga
     1741 tgctcaagaa cgggctcgcg gtggcggagg aagccgccga cagctagagt ctgttcccga
     1801 gcggcaccgg cgcgctcagc ccatgatgaa ggcgttgagt ttattgccgt cggggtcgcg
     1861 gaaatagccg gcgtagaaac cttcgccgcg cggtcccggg gggccttcgc aggtgccgcc
     1921 gtttgccagc gcgatgtcgt agaggcgctg gacctgttcc tgatcctttg ccgcgagcgc
     1981 gaccatcacg ccgttgccga ccgacgcggc attgccgtcg aacggcttgg tcaggccgat
     2041 ccccgcaccg ccgtccggct tgccccaagc gatgaagctt tcatattcca tcatccgcgg
     2101 cgtttcgagt tcggcggcga tcgcatcgta aaaggcggcc gcgcgcgcca gatcgttcgt
     2161 tcccagcgtc acatatccga tcatgatcca ttctcccgac tcgttggtcc gcgaacataa
     2221 taggaacatt gcccgcgccg ggcaaatgaa aagggcgccg cgacgaa
//
LOCUS       DATMSU010000536         3151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_84351, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000536 DATMSU010000000
VERSION     DATMSU010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84351"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VN034_14405"
     CDS             <1..252
                     /locus_tag="VN034_14405"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWV61851.1"
                     /translation="AAVAGHGIAIGSPILFSDEIAAGRLVPAHDLVAGDGRAFWFVYP
                     VARLQSAKIARFRDWLTETAATEVAAARTLVERAVILDP"
     gene            413..919
                     /gene="ybeY"
                     /locus_tag="VN034_14410"
     CDS             413..919
                     /gene="ybeY"
                     /locus_tag="VN034_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWV61852.1"
                     /translation="MLSVETHEATLWPDALDWEVRAAEAAAAALALTPFASLADAAPL
                     VEIAIRLTDDGEVHRLNRDFRGKDKPTNVLSFPQVQDDLLEGLANSDDGEILLGDIVL
                     ARETCMREAEEKGISVADHATHLIVHGTLHLVGYDHMDDASAAAMEALEVKALASLGI
                     ANPYADQD"
     gene            938..1882
                     /locus_tag="VN034_14415"
     CDS             938..1882
                     /locus_tag="VN034_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWV61853.1"
                     /translation="MPDDQGSSNRSEEDSSRSGLLSGLRNLLFGGDKEPSLREQIEEV
                     IDEAEEEGDDRRGSNIVGDLSSIERKMLRNLLHFGEQTVDDVAVPRADIIAISESAPF
                     TEIVALFAEAGHSRLPVYRENLDEVVGMIHVKDVFAVLAEGRPPPPLIDLIRQPLYVP
                     QSMGVLDLLAEMRAKRTHLAIVIDEYSGTEGLLTIEDLVEEIVGEIEDEHDDEPEPLI
                     VAGENGCWDADARAELDDVGEAIDPRLADVDEDVDTLGGLAAVLAGRVPEVGEILLHP
                     SGWRIEVIEADERRVHRLRLHPPVEVDLEAPSERTEEF"
     gene            1924..3081
                     /locus_tag="VN034_14420"
     CDS             1924..3081
                     /locus_tag="VN034_14420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWV61854.1"
                     /translation="MEVSDLRIALFSGNYNMTTDGANKALNRLVGFLLAHGAAVRVYS
                     PTVAHPDFEPTGDLVSVPSMAIPGRSEYRIPLSFSPRVRQDITAFDPNIVHISSPDRV
                     SRQAAAWARRRRLPVACSVHTRFETYFRYYNLSFLEPVVVAWLRKLYRKCDALIAPSE
                     SFAQVLRDQRMNYDIGIWTRGVEQAVFNPGRRDMAWRRSLGIGDDVPAIAFLGRLVME
                     KGLDVFADAIDVLQRRGVPHKVVVIGEGPAREWFESRLPDASFVGFQGGEDLAHALAS
                     CDIFFNPSVTETFGNVTLEAMACGLPVVAARATGSASIVKHGQTGYLVAPGSITSFAD
                     HLQHYCRDTRLRAEHGAAAVRESGAYQWDAINQAVADTYIRLIRQKQRRRS"
BASE COUNT          554 a         1033 c         1021 g          543 t
ORIGIN      
        1 gccgccgtcg cggggcacgg catcgcgatc ggatcgccga tccttttcag tgacgagatc
       61 gcggccggcc gcctcgtccc ggcgcacgat ctcgtcgcgg gcgacggccg cgccttctgg
      121 ttcgtctatc ccgtcgcacg gctgcagagc gcgaagatcg cgcgcttccg cgactggctg
      181 accgagacgg ccgcgacaga ggtcgcggcg gcgcggaccc ttgtcgagcg cgcggtcatt
      241 ctcgacccgt aaggcgcgtc aaatatcgct gtcctataaa atcgcgccgc gatacgagat
      301 ggcgagaaga gctttcaccg catcgcggcc gttcgcgcga catagggaca aacatgcgaa
      361 tcttgagtga agttgcgcag ttttcagcca gtttccgttt caggcttttt tcatgctgag
      421 cgtcgaaacc catgaggcaa ccctttggcc cgacgccctc gattgggagg tgcgtgctgc
      481 ggaggccgcc gcggcggcgc tcgcgctgac gcccttcgcg agcctcgctg acgccgcgcc
      541 gctggtcgag atcgcgatac gccttaccga cgatggcgaa gtgcacaggc tcaaccgcga
      601 tttccggggc aaggacaagc cgaccaacgt gctgtccttt ccgcaggtgc aggacgacct
      661 gctcgaaggt ctggcgaaca gcgatgacgg ggaaatcctg ctcggcgaca tcgtactcgc
      721 gcgcgaaacc tgtatgcgcg aggccgaaga aaagggcatt tcggtcgccg atcacgcgac
      781 gcacctgatc gtccacggca cgctccacct cgtcggatat gaccatatgg acgatgcctc
      841 ggcggcggcg atggaggcgc tcgaagtgaa agcccttgca tcgctgggca tcgccaatcc
      901 atatgcggat caggattaat ttaggatatg ccggaaaatg cccgacgacc agggatcttc
      961 gaaccgatcg gaagaggaca gtagtaggtc tggactgctg tccggactgc gaaacctgct
     1021 gttcggtggc gacaaggaac cgtcgctgcg cgaacagatc gaagaggtca tcgacgaggc
     1081 cgaagaagaa ggcgacgacc ggcgcggcag caatatcgtc ggcgacctgt cgtccatcga
     1141 gcgcaagatg ctgcgcaatc tcctccattt cggcgaacag acggtcgacg atgtcgcggt
     1201 gccgcgcgcc gacatcatcg ccatttcgga aagcgcgccc ttcaccgaaa tcgtcgcact
     1261 ctttgccgaa gcggggcaca gccgcctgcc cgtctatcgc gaaaatctcg acgaggtcgt
     1321 cggcatgatc cacgtcaagg acgtgttcgc ggtgctcgcc gaggggcgac cgccgccgcc
     1381 gctgatcgac ctgatccgcc agccgctcta tgtcccgcaa tcgatggggg tgctcgacct
     1441 gctcgccgaa atgcgtgcga agcgcaccca cctcgccatc gtcatcgacg aatattcggg
     1501 caccgaaggc ctgctgacga tcgaggatct ggtcgaggaa atcgtcggcg agatcgagga
     1561 cgagcatgat gacgagcccg agccgctgat cgtcgcgggc gaaaatggct gctgggacgc
     1621 cgacgcgcgc gccgagctcg acgatgtcgg cgaggcgatc gacccccggc tggccgacgt
     1681 cgacgaggat gtcgacacat tgggcgggct cgccgcggtc ctcgcggggc gcgtgcccga
     1741 ggtcggcgag attctcctcc acccgagcgg ctggcggatc gaggtgatcg aggccgacga
     1801 gcgccgcgtc caccgtttgc ggcttcaccc gcccgtcgag gtcgatctcg aagcgccgtc
     1861 ggagcggacc gaggagttct gacgcaattg cctttgcggg gctaaaggat taggcgcgac
     1921 cggatggaag tttccgacct tcgcatcgcc ctgttcagcg gcaattacaa tatgaccacc
     1981 gacggtgcga acaaggcgct caatcgcctg gtcggctttc tgctggcgca tggcgccgcg
     2041 gtgcgggtct attcgccgac cgtcgcccat cccgatttcg agccgaccgg cgaccttgtc
     2101 agcgtgccgt cgatggcgat cccggggcgg agcgaatatc gcattcccct gagcttttcg
     2161 ccgcgcgtcc gccaggacat caccgcattc gatcccaata tcgtccatat ctccagcccc
     2221 gaccgcgtct cgcggcaggc ggcggcctgg gcgcggcggc ggcggctgcc ggtggcctgc
     2281 tcggtgcaca cgcgcttcga aacctatttc cgctattata atctgtcctt cctcgaaccc
     2341 gtcgtcgtcg cctggctgcg caagctttac cgcaagtgcg atgcgctgat cgcaccgtcc
     2401 gaaagcttcg cgcaggtgct gcgcgaccag cggatgaatt acgacatcgg catctggacg
     2461 cgcggcgtcg agcaggcggt cttcaatccc ggccggcgcg acatggcgtg gcgccgctcg
     2521 ctcggcatcg gcgatgacgt gcccgcgatc gcctttctcg gccggctcgt gatggaaaag
     2581 ggactcgacg ttttcgccga cgccatcgat gtgctccagc ggcgcggcgt gccgcacaag
     2641 gtcgtcgtga tcggcgaggg gccggcgcgc gaatggttcg aatcgcgcct gcccgacgcc
     2701 agtttcgtcg gctttcaggg cggggaggac ctcgcccacg cgctcgcttc gtgcgatatc
     2761 ttcttcaacc cgtcggtcac cgaaaccttc ggcaatgtga cattggaagc catggcgtgc
     2821 gggctgcccg tcgtagccgc ccgcgccacc ggcagcgcga gcatcgtcaa gcacggacag
     2881 acgggttatc tcgtcgcccc cggatcgatc accagctttg ccgatcatct ccagcattat
     2941 tgccgcgaca cccggctgcg cgccgaacat ggcgcggcgg cggtgcgcga aagcggcgcc
     3001 tatcaatggg acgcgatcaa ccaggcggtc gccgatacct atatccgcct gatccgccag
     3061 aaacagcggc ggcgatcctg acgatggccg gggacctgtt cggcgagggt gcacccgagc
     3121 agcgcgacag cggcgcggcc ggccccgcgc c
//
LOCUS       DATMSU010000537         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_132454,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000537 DATMSU010000000
VERSION     DATMSU010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132454"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..2443)
                     /locus_tag="VN034_14425"
     CDS             complement(86..2443)
                     /locus_tag="VN034_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61855.1"
                     /translation="MIRTFSLHSAAALALAAALAAAPAAAQDAPLPIAEEDRGDGPIT
                     TSQEIVVLGSVGYRGRTEEAEPVLSYDSEFFQRFEPLTAGDALKRVPSVTFLSDVIES
                     DGARLRGLSPGYTQILINGERVPGTNNDRSFFMDRIPAELIDRVEIVRSSSARRTGDA
                     VAGTINIQLRDGYELDGGYVRAGGLYFDDKELEPSLGLVYGGAVGPGRLLVGLNLQGR
                     HNPKRKSSLRYGDSPENDPDFAVNEFDNREDQRDTRDGKDYSANATYEIDGETTDFRI
                     SGFYVRTDRTETERSFEYDDPTAITGPLPAGNLISDNSNVNEIDQENYSIDAKLSHEW
                     TLGKTALKVGFARFDDKQRETENQIDFDVDFDEGEVPVFEQVFTATDIVDKEFTVKLD
                     HEIELGNDIQFVAGGYYQKKKRNTAILDADGETEFPGLESSYDQFADNPDDLVSPFDP
                     LEPTTGGLNRIEERRLDGFALVQGKSGGLKWEAGIRYETTKLDIVDFSDPDDILAVDN
                     DYEKWLPSASIKFDLTPRDRITASVARTNRRPDFNYISPALLEEEVGDSDLLGNPFLA
                     PEMSWGVDLGYERRIGRTGVAGINFFYRDITNLIELVNTGEEGSEGEGTFIYQPQNVG
                     DGKVYGVEFDLSTDLGFIGLPNTGIFGNVSWLDSEITDFFGKRRFNGQSDYVYNVGFI
                     QDIPSWGVAFGATHRKQGAAYDRVIGEEIRTTYGADLELFVEKRIGGNFTIRAVGSNL
                     LNGKKREAFNKFDNLADQVDRNFDEYELESEKAGPVFQLMARYAF"
     gene            2663..4084
                     /locus_tag="VN034_14430"
     CDS             2663..4084
                     /locus_tag="VN034_14430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWV61856.1"
                     /translation="MIRKRRSFHFCRPLLAAVLVGTAGVAAAADTPPIPRSPGLPYAP
                     KNLPDRIVLTPGADPAHEMAVAFRTDVGQATAEAQLAVSVDGPTLEEKAALITGTSTP
                     VASSYGPSLYHQLRFTGLTPDTVYAYRVKGAAGWSEWYQFRTAAAEAKPFRFLYLGDV
                     QNGILTYASRVIRQAFHAHGDIRLTVHAGDLVAQRDDLDHDDEWGEWNAAGGYNYAIV
                     PQLPATGNHEYVEVVQPDGSEVYQLGPYWPAQFALPANGADPVKATTYYVDYQGVRFI
                     VLDGTAAIDLGTMQAQTDWLDRTLASSKANWNVVLFHQPVFTCARPNDTREIKAAWKP
                     VFDKRKVDLVLQGHDHCYSRLTSEAGREASAKARADGSVQGPVYLVSVTGSKMYRLND
                     RAGRQPDKIAEATELYQIVDVEPQRLKFRTYTASGRLYDGFDLERTAAGNRLHEISEP
                     TIAARRCSGEVGPDGGRCVARGK"
BASE COUNT          727 a         1260 c         1363 g          786 t
ORIGIN      
        1 atggcagctc tccaccccaa accggacgtt cacagggatt tcgcggccag cgcccaaggg
       61 actgggcgcc ggccgtcgaa ccctcttaaa aggcgtaacg cgccatcagc tggaacaccg
      121 gccccgcctt ttcgctttcg agttcatatt cgtcgaaatt gcggtcgacc tggtctgcga
      181 gattgtcgaa cttgttgaac gcctcgcgct tcttgccgtt gagcaggttc gacccgaccg
      241 cgcggatcgt gaaattgccg ccgatccgct tttcgacgaa gagttcgagg tcggcgccat
      301 aggtggtgcg gatttcctcg ccgatcacgc ggtcataggc cgcgccctgc ttgcggtgcg
      361 tcgccccgaa agcgacgccc cacgacggaa tgtcctggat gaagccgaca ttgtagacat
      421 agtccgactg accgttgaag cggcgcttgc cgaagaagtc ggtgatctcg ctatcgagcc
      481 acgagacgtt gccgaagatg ccggtattgg gaaggccgat gaaaccgagg tcggtcgaca
      541 ggtcgaattc gacgccatag accttgccgt cgccgacatt ctgcggctgg taaatgaagg
      601 taccctcgcc ttccgatccc tcttcgcccg tgttcaccag ttcgatgagg ttggtgatgt
      661 cgcggtagaa gaagttgatg cccgcgacgc cggtgcggcc gatgcggcgc tcgtagccga
      721 ggtcgacgcc ccacgacatt tcgggcgcga ggaacggatt gccgagcaga tcgctgtcgc
      781 cgacttcctc ttcgagcaat gcgggcgaga tatagttgaa gtcggggcgg cggttggtgc
      841 gcgcgaccga cgcggtgatg cggtcgcgcg gggtcaggtc gaacttgatc gacgccgacg
      901 gcagccattt ttcataatcg ttatcgaccg ccagaatatc gtcgggatcg ctgaaatcga
      961 cgatgtcgag tttcgtcgtt tcatagcgga tacccgcttc ccatttcagc ccgcccgatt
     1021 tgccctgcac cagcgcgaag ccgtcgagac gccgttcctc gatccggttg aggccgccgg
     1081 tcgtcggttc gagcggatca aagggcgaga cgaggtcgtc gggattgtcg gcgaactggt
     1141 cgtagctcga ttcgagcccc ggaaattcgg tttcgccatc ggcgtcgagg atcgcggtgt
     1201 tgcgcttctt cttctgataa tagccgccag cgacgaactg gatgtcgttg ccgagctcga
     1261 tttcatggtc gagctttacg gtgaattcct tgtcgacgat gtcggtcgcg gtgaagacct
     1321 gttcgaacac cggcacttcg ccttcatcga aatcgacgtc gaagtcgatc tggttctcgg
     1381 tctcgcgctg cttgtcgtcg aagcgggcga aaccgacctt cagcgcggtt ttgccgagcg
     1441 tccattcgtg cgacaatttg gcgtcgatgc tgtaattttc ctgatcgatc tcgttcacat
     1501 tgctgttgtc gctgatcaga ttgccggcgg gcagcggacc ggtgatcgcg gtcggatcgt
     1561 catattcgaa gctgcgctcg gtctcggtgc ggtcggtgcg gacatagaag cccgagatgc
     1621 ggaaatcggt ggtttcgccg tcgatttcat aggtcgcgtt cgccgaataa tccttgccgt
     1681 cgcgcgtgtc gcgctggtcc tcgcgattgt cgaattcatt gaccgcgaaa tcggggtcgt
     1741 tctcgggcga atcgccgtag cggagcgagc ttttccgctt ggggttgtga cgcccctgga
     1801 ggttgaggcc gacgagcagg cggccggggc cgaccgcgcc gccataaacg aggccgaggc
     1861 tgggttcgag ttccttgtcg tcgaaataga ggccgcccgc gcggacatag ccgccgtcga
     1921 gttcatagcc gtcgcgtagc tggatgttga tcgtgcccgc gaccgcgtcg ccggtgcggc
     1981 gcgccgacga cgagcggacg atctcgaccc ggtcgatcag ctcggcgggg atgcggtcca
     2041 tgaagaagct gcggtcgttg ttggtgccgg ggacacgctc gccgttgatc aatatctgcg
     2101 tatagccggg cgaaaggccg cggagccgcg cgccgtcgct ttcgatgacg tccgacagaa
     2161 aggtcaccga cggcacgcgt ttcagcgcgt cgcctgctgt cagtggctcg aaacgctgaa
     2221 aaaattcgct gtcgtacgaa aggacgggtt cggcctcctc ggtgcggccc cgatagccga
     2281 cgctgccgag aacgacgatt tcctgcgagg tcgtgatcgg gccgtcgccg cgatcctctt
     2341 cggcgatggg aagcggcgca tcctgcgcgg cggcgggggc ggcggcaagc gccgcggcaa
     2401 gggcgagagc ggcggcggaa tgcaggctga acgtgcgaat catatcgtag accccctttg
     2461 atggttgccg ggcctctagg ggggacgtgt gacagtttgc cgcgcgctat gtgaccgatc
     2521 gatgacggtt aagtgacgga aatattgtgg tattgacgca ggcgaacttt actggaacgg
     2581 ggccgggcgc acagcggttc gaaggcgatt tcgggcgctc gcgaagccgg cattgccgac
     2641 aagagggggc agtgaagacg ctatgattcg gaaaagacgc agctttcatt tctgccgccc
     2701 gttgctggcg gcggtactcg tcggcaccgc gggggtggcg gcggccgcgg acactccgcc
     2761 gattccccgc tcgcccggcc ttccctatgc ccccaaaaat cttcccgacc gcatcgtcct
     2821 gactcccggt gccgatccgg cgcacgaaat ggcggtcgct ttccgcaccg acgttgggca
     2881 ggcaaccgcc gaagcccagc tcgcggtgtc ggtcgacgga ccgacgctcg aggaaaaggc
     2941 ggcgctgatc accggcacat cgacacccgt cgccagcagc tatggcccgt cgctctatca
     3001 ccagctccgt ttcaccgggc tgacgcccga caccgtctat gcctatcgcg tcaagggcgc
     3061 ggcggggtgg agcgaatggt atcagttccg caccgccgcc gctgaagcga agccctttcg
     3121 cttcctctat ctcggcgacg tccagaacgg catattgacc tatgccagcc gcgtgatccg
     3181 gcaggccttt cacgcgcatg gcgacatccg gctgaccgtc cacgcgggcg acctcgtcgc
     3241 ccagcgcgac gacctcgacc atgacgacga atggggcgag tggaacgcgg cgggcggcta
     3301 taattatgcg atcgtcccgc agctgcccgc gacgggcaat cacgaatatg tcgaggtggt
     3361 gcagccggac ggcagcgagg tctaccagct cggcccctat tggccggcgc agttcgcgct
     3421 gcccgccaat ggcgccgatc cggtcaaggc gaccacctat tatgtcgatt atcagggggt
     3481 gcgtttcatc gtcctcgacg gcaccgcggc gatcgacctc ggcacgatgc aggcgcagac
     3541 cgactggctc gaccgcacgc tcgcctcgtc gaaggcgaac tggaatgtcg tgctgttcca
     3601 ccagccggtt ttcacctgcg cgcggccgaa cgacaccagg gaaatcaagg cggcgtggaa
     3661 gcccgtgttc gacaagcgca aggtcgacct ggtgctgcag ggccacgatc attgttatag
     3721 ccggttgacg tcggaagccg gccgcgaggc gagcgcgaag gcgcgcgccg atggcagcgt
     3781 ccaggggccc gtctatctgg tgtcggtgac cgggtcgaaa atgtaccggc tcaacgaccg
     3841 cgcgggccgc cagcccgaca agatcgccga ggcgaccgag ctgtatcaga tcgtcgatgt
     3901 cgagccgcag cggctgaagt tccgtaccta taccgcgtcg ggccggctgt acgacggctt
     3961 cgatctggag cggacggcgg ctggcaaccg ccttcacgaa atcagcgagc cgacaatcgc
     4021 ggcacgccgc tgttcgggcg aggtgggtcc cgacggcggc cgctgcgtcg cgcgcggcaa
     4081 atagatcagg acgaggggga acagatgatc cgcatgacga aatcgctcac cgccgc
//
LOCUS       DATMSU010000538         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_75516, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000538 DATMSU010000000
VERSION     DATMSU010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75516"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VN034_14435"
     CDS             <1..735
                     /locus_tag="VN034_14435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61857.1"
                     /translation="TAVVTGAASGIGAALARHAAAQGMTIAACDRDAPGLETLAAELA
                     AADVAHAVRPLDISDADALRSFADECAALPPIALLFANAGVLRGGSILEMPIDAWRLL
                     TEVNVIGAVATLQAFVPAMIALGEPAHVVVTGSTGSMTCGPSLGAYCATKHALWPIVE
                     TLRSELEGTTVGASLLMPGAVSTPMFDAIDPSRPTPADSLSPATVAERAFAGAQAGHA
                     KILTHPAYVERIGARFGAVLTELSEG"
     gene            complement(743..1747)
                     /locus_tag="VN034_14440"
     CDS             complement(743..1747)
                     /locus_tag="VN034_14440"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWV61858.1"
                     /translation="MNVPSRFGTRYPFHPYPTGWFSVGFADDLAPCDVRPLRYFGRDL
                     VLFRTESGEAVVTDAHCPHLGAHLGYDSRVEGETIVCPFHQWRYDTSGKCVHVPFAKA
                     VPPRAKVRKWPTVERGGMIFIWHDLDGRDADWNLPEYDESRRIPDAGFRRLHDDFGSA
                     HPQDVFENGVDFAHFPGVHGTGRAVGNGQIISDGHRFFSPVRILPAGYDGPIEGVDVG
                     STVDSEVIGGGLSRVESRTPHAPGIITVYYVSVTPVDAALSHYYVHQFFLKDDDCPMS
                     GEEIDRFTVLAAEHGLKEQYSDGKIWPYKAYVEQPMLSAADGPILKYRDWYRQFHPAV
                     "
     gene            complement(1769..2554)
                     /locus_tag="VN034_14445"
     CDS             complement(1769..2554)
                     /locus_tag="VN034_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61859.1"
                     /translation="MTTTPLKNLFDLTGRTALVTGGSRGLGLAMAEALGEYGARVILT
                     ARKQAELDEAVAHLQALGIDASAIATDVGNPDGPAHVVETVKSAGGRIDILVNNAGTS
                     WGAKTEDMPLDGWNKLMAVNLTGPFLMAQAVAREFMIPAGWGRILNIASVEGLLGHHP
                     RMIGTIAYNASKGGLVNFTRALAAEWGALGINVNALAPGYFPSKLTGYVLDKHGDELR
                     ADTPRGKLGTDDDLKGAVLLLASDAGAHITGQILAVDGGASII"
     gene            complement(2567..>3254)
                     /locus_tag="VN034_14450"
     CDS             complement(2567..>3254)
                     /locus_tag="VN034_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61860.1"
                     /translation="LAMAECGADIALVARNRSKLEAAAADVEKAGRRALIVEADVTDP
                     AAMVEAAKEIETALGRIDILFNNAGITNPQSVLDVAPQDFLRVQEVNIAGAFHTIRAF
                     APSMIAQGYGRIINMGSILSGRGMANRGAYCASKAGLANLGAACAFEFGGHGVTVNTL
                     GATVIVTDLNRDLIRTQPGLYAKVVDRTPLGRLGEVEDLMGALLFLASPASGFVTGQT
                     LFVDGGYTAG"
BASE COUNT          551 a         1046 c         1080 g          577 t
ORIGIN      
        1 acagcggtcg tcacgggggc ggcgtcgggg atcggcgccg cccttgcgcg tcatgccgcg
       61 gcacagggga tgacgatcgc ggcgtgcgat cgcgacgcgc cagggctaga gacactggcg
      121 gccgaactgg cggcggccga cgtcgcgcat gccgtccgcc cgctcgatat ttccgacgcc
      181 gatgcgttgc gcagcttcgc cgacgaatgc gccgccctgc cgccgatcgc gctgctcttc
      241 gccaatgcgg gcgttctgcg cggcggaagc atactcgaaa tgccgatcga cgcatggcgg
      301 ttgctgaccg aggtcaatgt catcggcgcg gtcgcaacgc tccaggcttt cgtccccgct
      361 atgatcgcgc ttggcgaacc ggcgcatgtc gtcgtgaccg gctccacggg gtcgatgacg
      421 tgcggtccgt cgctgggcgc ctattgcgcg accaaacatg cactctggcc catcgtggaa
      481 accttgcggt ccgaattgga gggcacgact gtcggcgcct cgcttctgat gcccggcgcg
      541 gtatcgaccc cgatgttcga tgcgatcgat ccctcccggc cgaccccggc ggattcactg
      601 agccctgcca ccgtcgccga acgtgctttc gcgggggcgc aggcaggtca tgccaagatc
      661 ctgacgcacc ccgcttatgt cgagcggatc ggcgcacggt tcggcgcggt gttgaccgag
      721 ctttcggaag gttgatccgc cgtcagacgg cgggatggaa ctgcctgtac cagtcgcgat
      781 atttgaggat cggaccatcg gcggcgctca gcatcggctg ctcgacatag gctttgtacg
      841 gccagatctt tccgtcgcta tattgttcct tcagcccgtg ctcggcggcg agcacggtga
      901 agcgatcgat ctcttcaccc gacatcgggc agtcatcgtc cttcaggaag aattgatgga
      961 cgtaataatg gctgagcgcc gcatcgaccg gggttaccga gacataatag acggtgatga
     1021 tccccggagc gtgtggcgtc cggctctcga cacgcgagag cccgccgccg atcacctcgg
     1081 aatcgaccgt cgatccgaca tcgacgccct cgatcgggcc atcatagccc gcgggaagga
     1141 tgcgcaccgg gctgaagaag cgatgcccgt ccgatatgat ctgtccgttg ccgaccgcac
     1201 ggccggtgcc atgaacgcca gggaaatgcg cgaaatcgac gccattctcg aacacgtcct
     1261 gtggatgagc cgaaccgaaa tcgtcgtgga ggcggcggaa acctgcgtcg ggaatgcgtc
     1321 gactttcgtc atattcggga agattccagt cggcgtcgcg tccgtccaga tcgtgccaga
     1381 tgaagatcat gccaccgcgc tcgacggtcg gccatttgcg gactttcgcg cgtggcggca
     1441 cggctttcgc gaagggcaca tggacgcatt tgccgcttgt gtcgtagcgc cactggtgaa
     1501 acgggcagac gatcgtctca ccctcgaccc ggctgtcgta gccgagatgc gcgccgaggt
     1561 gcggacaatg cgcgtcggtc acgaccgcct cgccgctttc ggtgcggaag agcacgagat
     1621 cgcgtccgaa ataacgaagc ggacgaacgt cacaaggcgc aagatcatcg gcaaagccga
     1681 cactgaacca gcccgtcgga tagggatgaa agggatagcg ggtgccgaag cggctcggta
     1741 cgttcatgtc tctctcccca ttcgttgctc agatgatcga cgcgcccccg tcgaccgcga
     1801 gtatctgccc cgtgatatgc gcgccggcgt cggaggcgag cagcagcacc gcgcctttca
     1861 gatcgtcgtc ggtgcccaac ttgccgcgcg gtgtgtccgc gcgcagttca tcgccatgtt
     1921 tgtcgaggac atagccggtc agcttcgacg gaaaatagcc gggcgcgagc gcattgacgt
     1981 ttatgccgag cgcgccccat tcggccgcaa gcgcgcgggt gaagttgacg aggccgccct
     2041 tgctcgcgtt gtaggcgatg gtgccgatca tccgcggatg atggccgagc agcccctcga
     2101 ccgaggcgat attgaggatg cgtccccagc ccgcggggat catgaactcg cgtgcgaccg
     2161 cctgcgccat caggaagggg ccggtgaggt tcaccgccat cagcttgttc cagccgtcga
     2221 gcggcatgtc ctcggtcttg gcgccccatg acgtgccggc gttgttgacg aggatgtcga
     2281 tccgcccgcc cgcgctcttc accgtctcga cgacatgcgc cgggccgtcg ggattgccga
     2341 catcggtagc gatcgcgctc gcgtcgatgc caagcgcctg aagatgcgcg accgcctcgt
     2401 cgagttcggc ctgcttgcgc gcggtcagga tgacgcgcgc gccatattcg cccagcgcct
     2461 cggccatcgc gaggccgagc ccgcgcgaac cgccggtgac gagcgcggtg cgaccggtga
     2521 ggtcgaacaa attcttgagc ggtgtcgtgg tcatgctttt tccaattcaa ccagcggtgt
     2581 aaccgccgtc gacgaacaag gtctggccgg tgacgaagcc cgacgcgggc gatgcgagga
     2641 agagcagcgc gcccatcaga tcctcgacct cgccgaggcg gccgagcggg gtgcgatcga
     2701 cgaccttcgc gtaaaggccg ggctgggtgc ggatgaggtc gcggttgagg tcggtgacga
     2761 tcaccgtcgc gcctaacgtg ttgacggtaa cgccatggcc gccgaattcg aacgcgcagg
     2821 ccgcgccaag gttggccagc cccgccttcg acgcgcaata ggcgccgcgg ttcgccatgc
     2881 cgcgccccga caggatcgag cccatgttga tgatccggcc atagccctgc gcgatcatcg
     2941 acggcgcgaa ggcacggatc gtgtggaacg cgcccgcgat attgacctcc tgcacacgca
     3001 ggaaatcttg cggagccacg tcgagcaccg attgcggatt ggtgatgccg gcgttgttga
     3061 acaggatgtc gatgcggccg agcgcggttt cgatttcttt cgcggcctcg accatcgcgg
     3121 cgggatcggt gacatcggcc tcgacgatca gcgcgcggcg ccccgccttt tcgacgtcgg
     3181 ctgccgcggc ttcgagtttc gagcggttgc gcgccacgag cgcgatgtcg gcgccgcact
     3241 cggccatcgc gagc
//
LOCUS       DATMSU010000539         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_47357, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000539 DATMSU010000000
VERSION     DATMSU010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47357"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VN034_14455"
     CDS             complement(<1..577)
                     /locus_tag="VN034_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61861.1"
                     /translation="MYSESDLQAAVDAKVLTPEAATAFRSHIASVRAAPGADEESFRL
                     ITGFNDIFVSIAAVILLVAVGWIGASIHTALGGAFVAASAWFLAEYFTRKRRMALPSI
                     VLVLAFSGGVFATMVGFLVRHGEDIFGHSPSETVGAILVGAMALVTAAATWAHWKRFM
                     VPITVAAGTAALAATAVALVLAITGMPSPDGT"
     gene            complement(814..>1628)
                     /locus_tag="VN034_14460"
     CDS             complement(814..>1628)
                     /locus_tag="VN034_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61862.1"
                     /translation="GAKVVVNDLGGSRDGTGHSDAALQVVEEIRAAGGTAMSNGGSVT
                     EYEQMVEMVAKAKEEWGGVHVLINNAGILRDKSFAKMEPADFDLVLKVHVSGSANVTK
                     ACWELMREQAYGRILMTASSTGLYGNFGQANYGAAKLGLAGLTKTLHLEGAKYNIRCN
                     TIAPVAGTRMTEDIFPAELFEKFTPENVVPAALYLVSEDAPTNMIVGAGAGAFHAAYV
                     TMTPGVALPEGERTPEGVAAAWEKIIDRNGETVPQSGSEQSMNVMKALMALG"
BASE COUNT          266 a          532 c          535 g          295 t
ORIGIN      
        1 gcgtgccgtc ggggctgggc attccggtga tcgcgagcac gagcgccacc gcggtcgccg
       61 cgagcgcggc ggtgccggcg gcgacggtga tcggcaccat gaagcgtttc caatgcgccc
      121 aggtcgccgc ggcggtaacc aacgccatcg cgccgaccag gatcgcgccg acggtttcgc
      181 tggggctatg tccgaaaata tcttcgccat ggcggacgag gaaaccgacc atcgtcgcga
      241 agaccccgcc cgaaaaggcg aggacgagga cgatgctggg cagcgccatg cggcgtttac
      301 gcgtgaaata ttcggcgagg aaccacgccg acgcggcgac gaaggcgccg ccgagcgcgg
      361 tatggatcga cgcgccgatc cagccgaccg cgacgagcag gatgaccgcc gcgatgctga
      421 cgaaaatatc gttgaagccg gtgatgaggc ggaatgattc ctcatccgct ccgggagcgg
      481 cacgaaccga tgcgatatgc gaccgaaaag ccgtggcggc ctccggcgtc agcactttcg
      541 catcgaccgc ggcttgcagg tcgctttcac tatacatcgg ggtcgtcctt ttcgtccgat
      601 ccccttgccc caccgctctt atcgcattgg cgtgttgctg tgtcaataca gtgaagcggg
      661 ttatatcctc cctgtggccg caggccatgg ggagggggac cgcgcccgaa gggcgtggtg
      721 gaggggccgc gacggtgcgc cattggcccc tccgtcagcg cttcgcgctg ccacctcccc
      781 atcgctccgc gacagggagg atccaatcaa acctcagccc agcgccatca atgccttcat
      841 gacattcatc gactgttcgc tgcccgattg cgggacggtt tcgccgttgc ggtcgatgat
      901 cttctcccac gccgcggcga cgccttcggg ggtgcgctcg ccttcgggaa gcgcgacgcc
      961 gggggtcatc gtgacatagg cggcgtggaa cgcgcccgcg cccgcgccga cgatcatgtt
     1021 ggtcggggca tcctcgctga ccagatagag cgcggcggga acgacattct cgggcgtgaa
     1081 cttctcgaac agttcggccg ggaagatgtc ttcggtcatc cgcgtgcccg cgacgggtgc
     1141 gatcgtgttg caacggatgt tgtacttcgc gccttcgagg tggagcgtct tggtcagccc
     1201 cgcgagcccc agcttcgccg cgccgtaatt cgcctgcccg aaattgccgt agaggccggt
     1261 cgacgaggcg gtcatcagga tgcggccata ggcctgctca cgcatcaatt cccagcacgc
     1321 cttggtgacg ttggcgctgc cgctgacatg cactttcagc accaggtcga aatcggcggg
     1381 ctccatcttg gcgaagctct tgtcgcgcag aatgccggcg ttgttgatca ggacgtggac
     1441 gccgccccat tcttccttgg ccttggcgac catttcgacc atctgctcat attcggtgac
     1501 gctgccgccg ttcgacatgg cggtgccgcc cgcggcgcgg atttcctcga cgacctgcag
     1561 cgcggcgtcc gaatggccgg tgccgtcgcg cgatccgccg aggtcgttga cgaccacttt
     1621 ggcgccgc
//
LOCUS       DATMSU010000540         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_94635, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000540 DATMSU010000000
VERSION     DATMSU010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94635"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="VN034_14465"
     CDS             complement(<1..585)
                     /locus_tag="VN034_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWV61863.1"
                     /translation="MNGRSKIRYGMVGGGEGAFIGAVHRMAAALDSEYELVCGAFSSD
                     ADRNGRSAGALGLDPRRAYATFDELLAAEAALPADERMEVLAIVTPNHLHAPMAIAAL
                     DAGFHVLSEKPMALNLAEAHAIAAAVARSGKLYGLAFTYSGYPLIEEARLRVARGDFG
                     RIRLVQVEYSQGWLSRPIDADGNKQAEWRTDPARA"
     gene            681..1067
                     /locus_tag="VN034_14470"
     CDS             681..1067
                     /locus_tag="VN034_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWV61864.1"
                     /translation="MKFLFALPLAGAILTAAMLMPSPQAHAGPQGAGAQAFAACKACH
                     TLEPGGKSVMGPNLHGLMGRQAGSVAGFKYSPALKASKIRWTEKSLDEYLAAPTKKVP
                     GTRMVVKVADPARRAALIAYLKTETK"
     gene            1084..2127
                     /locus_tag="VN034_14475"
     CDS             1084..2127
                     /locus_tag="VN034_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWV61865.1"
                     /translation="MKGPAVFLAQFMGDAAPFDTLPSAARWMADAGYVGIQIPTWDSR
                     CIDLAKAAESQDYCDELAGTCREAGVEITELSTHLQGQLVAVHPAYDAQFDAFAPDAL
                     KGKPAERQKWAVEQLGLAAKASRRLGLNAHATFSGALAWPYLYPWPQRPAGLVEEAFA
                     ELAKRWRPILDAFDEQGVDLAYEIHPGEDLHDGVTFERFLAGVDNHPRANILYDPSHY
                     VLQALDYLAFIDIYHERIRMFHVKDAELNPTGRSGVYGGFQDWVDRPGRFRSLGDGQV
                     DFGGIFSKLTQYGYSGWAVLEWECCLKHPEDGAREGAPFIAAHMIRKADKAFDDFAGG
                     SDPALNRTMLGLS"
     gene            2175..>3406
                     /locus_tag="VN034_14480"
     CDS             2175..>3406
                     /locus_tag="VN034_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWV61866.1"
                     /translation="MTTLHKGRLFWLGVLALFTAAASLAIRGAIASGLKAEWIDPVAP
                     LQAGELIAAALGAAFLSFAITLFVASALLDQIGMKRCLAGAGLCFILGPLMIVTAGHV
                     ASGMTIYWLVWTGMLLSGIGWGLTEAAINPLTAQLYPEDTTHRLNVLHAWFPGGIIVG
                     GLAGFFLSATLPWQGIMALVMIPAVATVIIAATTTFPPPLREQSGVSFGEMVGEVFRR
                     PSFFIWFGAMFLTAASELAPGQWIDVALSNRVGMRGILLLVYVNALMFVFRHFAGRLA
                     NRISNPGLLWVSSLLAAIGLFMLSQAQSPVAAIVASTVWGFGVCVMWPTMLASVAERY
                     PRGGSWAMGLVGSAGALASFFVLPQLGAMFDRAKIEFAGGPEAFAQLTGAAKLAVEDA
                     AASLSFQRLSILPVVLLAV"
BASE COUNT          543 a         1142 c         1098 g          623 t
ORIGIN      
        1 ggcgcgcgcg ggatcggtgc gccattcggc ctgcttgttg ccgtccgcat cgatcggccg
       61 gctcagccag ccttgcgaat attcgacctg caccagccgg atccggccaa aatcgccgcg
      121 cgcgacgcgc aggcgcgcct cctcgatcag cggatagccg ctgtaggtga aggcgagccc
      181 gtagagcttg ccgctgcgcg cgacggccgc ggcgatcgcg tgcgcctcgg ccagattcag
      241 cgccatcggc ttttcggata ggacatgaaa acccgcgtcg agcgccgcga tcgccatcgg
      301 ggcgtggaga tgattcgggg tgacgatcgc gagcacttcc atccgctcgt ccgcgggcag
      361 cgccgcttcc gccgcaagca attcgtcaaa ggtcgcatag gcgcggcgcg gatcgagtcc
      421 cagcgccccg gcgctccgcc cgttgcggtc ggcatcgctg ctgaacgcac cgcataccaa
      481 ttcatattcg ctatcgagcg cggcggccat acggtgcacc gcaccgatga aagcgccctc
      541 gccgccgccg accatgccat agcgaatttt cgaccgaccg ttcatcgcat tccctctgcc
      601 agaattcata aaattgtttg cattattcgc tttcaaacgg ttacatcatc gcaccggctg
      661 ttgccagacg ggagaggata atgaaatttc tgttcgccct gccattggcg ggtgccatat
      721 tgactgccgc gatgctcatg ccgtcgcccc aagcgcatgc cgggccccaa ggcgcgggcg
      781 cgcaggcctt cgccgcgtgt aaggcctgcc atacgctgga gcccggcggc aaaagcgtga
      841 tggggcccaa tctacacggg ctgatgggcc ggcaagccgg ctcggtcgcc ggattcaaat
      901 acagcccggc gctcaaggcc tcgaaaatcc gctggaccga aaagtcgctc gacgaatatc
      961 tcgccgcgcc gaccaagaag gtgccgggca cgcgcatggt cgtcaaagtg gccgatccgg
     1021 cgcggcgcgc ggcgctgatc gcctatctca agaccgaaac caagtgaccg ggaaggccat
     1081 cgcatgaagg gtccggccgt cttcctcgcg cagttcatgg gcgacgccgc gccgttcgac
     1141 actttgcctt cggcggcgcg ctggatggcg gatgcgggct atgtcggcat ccagattccg
     1201 acctgggaca gccgctgcat cgatctcgcc aaggcggccg agagtcagga ttattgcgac
     1261 gagctcgccg gcacctgccg cgaagcgggg gtcgagatca ccgaattgtc gacgcatctg
     1321 caaggccagc tggtcgcggt gcatcccgcc tatgacgccc agttcgacgc tttcgctccc
     1381 gacgcgctga aaggcaagcc cgccgagcgg cagaaatggg cggtcgagca gctcggcctt
     1441 gccgcgaaag cgagccggcg gctcggcctt aatgcccatg cgaccttctc gggcgcgctc
     1501 gcctggccct atctctatcc ttggccgcag cgtcccgcgg gactggtcga ggaagccttc
     1561 gccgaactcg cgaagcgctg gcggccgatc ctcgacgcct tcgacgagca gggtgtcgac
     1621 ctcgcctatg aaattcatcc cggcgaggac ctgcacgacg gcgtgacctt cgaacgtttc
     1681 ctcgcgggcg tggacaatca tccgcgcgcc aacatcctct acgaccccag ccattatgtg
     1741 ctgcaggcgc tcgattacct cgctttcatc gacatttatc acgagcggat ccgcatgttt
     1801 cacgtcaagg acgcggagct caatccgacc gggcgatcgg gggtctatgg cggctttcag
     1861 gactgggtcg atcgccccgg ccgcttccgc tcgctgggcg acggccaggt cgatttcggc
     1921 ggcatcttct cgaagctcac gcaatatggc tattccggct gggcggtgct cgaatgggaa
     1981 tgctgcctga agcatcccga agacggcgca cgcgaaggcg cgcccttcat cgccgcgcat
     2041 atgatccgca aggccgacaa ggcgttcgac gatttcgcgg gcggcagcga cccggcgctc
     2101 aaccgtacga tgttggggct gagctgacga cgcggcaaac aaattaaaac gataataatc
     2161 aaaggggacg caggatgacg acattgcaca aggggcgctt gttctggctc ggcgtgctcg
     2221 cgctgttcac cgccgcggcg agcctcgcga tccgcggtgc gatcgccagc gggctcaagg
     2281 ccgaatggat cgacccggtc gcccccttgc aggcgggcga gctgatcgcc gccgcgctcg
     2341 gcgccgcctt cctcagcttc gcgatcacgc tgttcgtcgc cagcgcgctg ctcgatcaga
     2401 tcggcatgaa gcggtgcctc gccggcgcgg ggctgtgctt cattctcggc cccttgatga
     2461 tcgtcaccgc gggccatgtc gcgtccggca tgaccatcta ttggctcgtt tggacgggca
     2521 tgttgctcag cggcatcggc tgggggctga ccgaggcggc gatcaatccg ctgaccgcgc
     2581 agctttaccc cgaagacacg acccaccggc tcaatgtgct gcacgcatgg ttccccggcg
     2641 gcatcatcgt cggcgggctt gccggcttct tcctctcggc gaccttgccg tggcagggca
     2701 tcatggcgct ggtgatgatc cccgccgtcg cgaccgtgat catcgccgct accacgacct
     2761 tcccgccgcc gctgcgcgag caaagcggcg tcagtttcgg cgaaatggtc ggcgaggtgt
     2821 tccgccgccc cagcttcttc atctggttcg gcgcgatgtt cctgaccgcg gcgtccgaac
     2881 tcgcccccgg gcaatggatc gacgtcgcgc tgtcgaaccg cgtcggcatg cgcggcatat
     2941 tgctgctcgt ctatgtcaac gctttgatgt tcgtcttccg ccatttcgcg gggcggctcg
     3001 ccaacaggat ttcgaacccc ggcctgctct gggtgtcgag cctgctcgcg gcgatcgggc
     3061 tattcatgct gagccaggca cagtcgccgg tggcggcgat cgtcgcctcg accgtctggg
     3121 gcttcggcgt ctgcgtgatg tggccgacga tgctcgcctc ggtcgccgaa cgctatccgc
     3181 gcggcggatc gtgggcgatg gggctggtcg gatcggcggg ggcgctggcg agctttttcg
     3241 tgctgccgca gctcggcgcg atgttcgacc gcgccaagat cgaattcgcg ggcgggcccg
     3301 aggcgttcgc gcagctgacc ggcgccgcca agctggcggt cgaggatgcc gccgcgtcgc
     3361 tgtcgttcca gcggctgtcg atcctgcccg tcgtcctgct cgcggt
//
LOCUS       DATMSU010000541         3169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_56629, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000541 DATMSU010000000
VERSION     DATMSU010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56629"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1529
                     /locus_tag="VN034_14485"
     CDS             <1..1529
                     /locus_tag="VN034_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HWV61867.1"
                     /translation="RIEALGARHMDGMSTNLAASLAMRMRSDPISRAQNREDVPISSA
                     LELMLREALTGEQAPQGTETGLSLVREWIGKEAGGDLTALSMLLDDQAAFAETAKLAL
                     RHLDLIHSDEPLEEGSEDGGEQDETEADESQEEQESEDGGAGESQVDARAEMAGDQAD
                     DGDSDPDAQEMEADGEPEMGGEGDEGMLPVRPNRLPSDIPDFNYLRFTEKHDEIVAAT
                     ELCDADELTRLRAYLDQQMTHLQGAVTKLANRLQRRLMAQQNRAWDFDQEEGQLDAAR
                     LARVIVSPGQSLSYKIERDTDFRDTVVTLLIDNSGSMRGRPISIAAISADILARTLER
                     CGVKTEILGFTTRTWKGGQSREDWLAAGRPAHPGRLNDLRHIIYKPADEPYRRARKSL
                     GLMMREGLLKENIDGEALMWAHQRIINRPEERRILMVISDGAPVDDSTLSVNHGAYLD
                     QHLRQVIEWIENRSPVELCAIGIGHDVTRYYSRAVTIMDAEQLGGTMVEQLAGLFDID
                     "
     gene            complement(1610..2053)
                     /locus_tag="VN034_14490"
     CDS             complement(1610..2053)
                     /locus_tag="VN034_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61868.1"
                     /translation="MQPIKFIAAAAVAAMIVSTPAVAKKKEAAYVVVNEEMGVPVFPH
                     DITDKPYKIVGVVTAGVRKATIFSKEPSQQKIYNEIWERGEKLGADAVIGAKYGDSHI
                     TALSWGNTKAGGVAIKFLTAEEIAAGTKGDTLEGFDPEAWTGKKK"
     gene            2256..2759
                     /locus_tag="VN034_14495"
     CDS             2256..2759
                     /locus_tag="VN034_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61869.1"
                     /translation="MATATPTTAEPTATVAPPPAPALLLRRDTPIHFMVVNEVTTKTH
                     TPGHRFKLRVDKPVVVDGATIIPIGATAWGEVLAAKKSGNVGKGGSLEARLLYVESNG
                     MQIPVSGTNNAKGASAGGETALGILALGPLGLFAKGNNAKIKAGELMTGFVEQDTEIP
                     RAVPTGL"
     gene            2756..>3169
                     /locus_tag="VN034_14500"
     CDS             2756..>3169
                     /locus_tag="VN034_14500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61870.1"
                     /translation="MTLLRFLPICMMLALPIAANSQNPIVTQPVPIQSGSHIIVPAGT
                     IIDLVTTNPISSKKSAKGDLLYLKVAESLTIEGATALPAGTVVVAQLTRAEQSGAFGR
                     SGKLDVQLLYAELPGGTVRVSGAVRARGKSGADDAA"
BASE COUNT          606 a         1023 c         1021 g          519 t
ORIGIN      
        1 cgcggatcga ggcgctgggt gcgcgccaca tggatggaat gagcaccaac ctcgccgcca
       61 gcctcgcgat gcggatgcgc agcgacccga tcagccgcgc gcaaaaccgc gaggacgtgc
      121 cgatttcgag tgcgctcgag ctgatgctgc gcgaggcgct gaccggcgaa caggcgccgc
      181 aggggaccga aaccgggctg tcgctggtgc gcgaatggat cggcaaagag gcgggcggcg
      241 acctcaccgc gctgtcgatg ctgctcgacg atcaggcggc gttcgccgaa accgcgaagc
      301 tcgcgctgcg ccacctcgac ctgatccaca gcgacgaacc gctcgaagaa ggcagcgagg
      361 acggcggcga gcaggacgag accgaggccg acgagagcca ggaagagcag gaaagcgaag
      421 acggcggcgc cggcgagagc caggtcgacg cgcgcgccga gatggcgggc gatcaggccg
      481 acgacggcga cagcgacccc gacgcacagg aaatggaagc cgacggcgaa cccgaaatgg
      541 gcggcgaagg cgacgagggc atgctccccg tgcgccccaa ccgcctgccg agcgacattc
      601 ccgatttcaa ctatctgcgc ttcaccgaaa agcatgacga gatcgtcgct gcgaccgaac
      661 tgtgcgacgc cgacgagctg acgcggctgc gcgcctatct cgaccagcag atgacgcatc
      721 tgcagggcgc ggtgaccaag ctcgcgaacc gcctccagcg ccggttgatg gcgcagcaga
      781 accgcgcgtg ggatttcgat caggaggaag gccagctcga cgccgcacgc ctcgcgcgcg
      841 tcatcgtctc gcccggccag tcgctctcat acaagatcga gcgcgatacc gatttccgcg
      901 acaccgtcgt cacgctgctc atcgacaatt cgggctcgat gcgcggacgg ccgatcagca
      961 tcgcggcgat cagcgccgac atcctcgcgc gcacgctcga acgctgcggc gtcaagaccg
     1021 aaatcctcgg cttcacgacg cggacgtgga agggcgggca gagccgcgag gactggcttg
     1081 ccgcgggacg cccggcgcac cccggccgcc tgaacgacct gcgccacatc atctacaagc
     1141 ccgccgatga accctatcgc cgcgcgcgca agtcattggg gctgatgatg cgcgaagggc
     1201 tgctcaagga aaatatcgac ggcgaggcgc tgatgtgggc gcaccagcgc atcatcaatc
     1261 gccccgagga acgccgcatc ctgatggtga tttcggacgg cgccccggtc gacgattcga
     1321 cgctgtcggt gaaccatggc gcctatctcg accagcattt gcgccaggtg atcgaatgga
     1381 tcgagaaccg ctcgccggtc gagctgtgcg cgatcggcat cggtcacgac gtcacgcgct
     1441 attacagccg cgcggtgacg atcatggacg ccgaacaatt gggcggcacg atggtcgagc
     1501 agctcgcagg gctgttcgac atcgattgac ggggcaaaaa aagggaacgg ccatgccgct
     1561 ccccttttcg tttcgtcgac cgtccagcct gtgtgtgtgt ggcccgtgct tatttcttct
     1621 tgcccgtcca cgcctcggga tcgaaaccct cgagcgtgtc gcccttggtc ccggccgcaa
     1681 tttcctcggc ggtcaggaac ttgatcgcga cgccgccggc tttggtgtta ccccagctca
     1741 acgcggtgat gtggctgtca ccatatttgg cgccgatcac ggcgtcggcg cccagttttt
     1801 cgccgcgttc ccagatttcg ttgtagattt tctgctgcga cggttccttg ctgaaaatcg
     1861 tcgccttgcg cacccccgcg gtcacgacgc cgacgatctt gtacggcttg tcggtgatgt
     1921 cgtgcgggaa gacgggcacg cccatttcct cgttgaccac cacataggcc gcttcttttt
     1981 tcttggcgac cgccggcgtc gaaacgatca ttgccgccac ggcagccgcg gcgataaact
     2041 tgataggctg catattgata ctccccctgt tggagcgctt tagttcgtcg cctctctggc
     2101 cgaggtcaag tccgattcta tattggcaaa tgctgccatt catggcctaa gtcgacgggg
     2161 atttactggg gggtatcatg gcttcaattc aagctgggat ggcagcactg tggttggttg
     2221 gggcagcgca gggcgcgccg caggcggcag aagcggtggc gaccgccacg ccgacgaccg
     2281 cagagccgac cgcgacggtc gctccgcctc cagcacccgc gctgctgttg cggcgcgata
     2341 ccccgatcca tttcatggtc gtgaacgaag tgacgaccaa gacgcacacg ccggggcacc
     2401 ggttcaaatt gcgcgtcgac aagccggtcg tcgtcgatgg agccacgatc atccccatcg
     2461 gtgccacggc gtggggcgaa gtgcttgcgg cgaagaaaag cggcaatgtc ggcaagggcg
     2521 gatcgctcga agcccgattg ctgtacgtcg aaagcaacgg gatgcagata cccgtcagcg
     2581 ggaccaataa cgccaagggc gccagcgcgg gcggcgaaac ggcgctcggc atccttgcgc
     2641 tcggtccgct cggcctcttt gccaagggca ataatgcgaa gataaaggcg ggcgaactga
     2701 tgaccggctt tgtcgaacag gataccgaaa tcccgcgcgc ggtaccgacc ggcctgtgac
     2761 tttgttgcgc tttctgccga tctgcatgat gctggcgctt ccgatcgcgg cgaacagcca
     2821 gaatccgatt gtcacgcagc cggtgccgat acaatccgga tcgcatatca tcgttccggc
     2881 cgggactatc atcgaccttg tcaccaccaa cccgatctca agcaagaaga gcgccaaagg
     2941 cgaccttctc tacctcaaag tcgcggagtc gctgaccatc gagggcgcga cggcgcttcc
     3001 ggccggcaca gtcgtcgtcg cccaactgac ccgcgccgaa caaagcggcg cgttcggacg
     3061 gtcgggcaag ctcgacgtac aattgctcta tgccgagctt cccgggggca ccgttcgcgt
     3121 cagcggcgcg gtccgggcgc gcggcaagag cggcgccgac gacgcggcg
//
LOCUS       DATMSU010000542         3658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_27841, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000542 DATMSU010000000
VERSION     DATMSU010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27841"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /gene="ftsA"
                     /locus_tag="VN034_14505"
     CDS             complement(<1..840)
                     /gene="ftsA"
                     /locus_tag="VN034_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWV61871.1"
                     /translation="MAPPRIEKIITALDVGSWKVCALIAGQTADGQLHVLGTGQRESR
                     GVQRGYVADMEQTEHIVREAIEQAERIAGLNIDDVWVSFSAGGLLSDVAPIESELGGH
                     RIEQEDIDDLLAAGRAGIDPEGRMILHAQPALYTLDGLNGVKNPIGLHADRLGVDIHI
                     IMADGAPVRNLEAAVRQAHLDVNAVVASPIAAGLACLSDEERDLGVALVELGAAVTTV
                     SLYAGGMLVEMASLPFGASDITDDIASAFGIRRSQAQRLQSFYGSASASPRDNNEIIE
                     LEPG"
     gene            complement(844..1782)
                     /locus_tag="VN034_14510"
     CDS             complement(844..1782)
                     /locus_tag="VN034_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HWV61872.1"
                     /translation="MSKTQIKRGKAPPRRPARAPKKRRKIQQSRLNAIINALPISPQR
                     LQKVANWTIGLSLCAVVAIAAHATGVTAKIHEEYAQAVGRAGFQVRKVEVVGADRIDR
                     LKVYDIALAQKDRSMAAVDLEDVRADLMKYGWIKDARVSRRLPDTLVVDIVERTPAAI
                     WQHNNRLSLIDEKGVVLEPVTVATMPDLPLVIGPKANQRSQDLDRLLTEASSLKELLA
                     GATWVGNRRWDLRFRSGETLSLPEGEAEAKAALAKFAHMDGANRLLGRGILRFDMRDP
                     ARFVLRLPHEGQVAPAKLDDARAAVDAVAEASAEKG"
     gene            complement(1779..2705)
                     /locus_tag="VN034_14515"
     CDS             complement(1779..2705)
                     /locus_tag="VN034_14515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWV61873.1"
                     /translation="MSRGPWHVAVLMGGWSAEREVSLMSGNGVADALESRGHRVTRID
                     MDRDVALRLAEAKPDVVFNALHGVPGEDGTVQGMLDLMGLKYTHSGLVTSVIAIDKEL
                     TKQALVPHGIPMPTGTMVDSESLFTVDPLPRPYVLKPVNEGSSVGVAIVKDDSNYGNP
                     IAREAVGPWQEFDRLLAEPFIKGRELTVAVLGGTALAVTELRVKSGFYDYDAKYTEGL
                     TEHVCPADVPDEVAQRMKDLAIQAHGLLGCKGVSRSDFRWDDEHGLAGIFLLEVNTQP
                     GMTALSLVPEQGRAVGMDYAELVERIVGEALS"
     gene            complement(2702..3598)
                     /gene="murB"
                     /locus_tag="VN034_14520"
     CDS             complement(2702..3598)
                     /gene="murB"
                     /locus_tag="VN034_14520"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWV61874.1"
                     /translation="MTTATLPTVRGKLTPNAPLAPLVWFKSGGPADWLFEPKDADDLA
                     DFLRDLDSAIPVMALGLGSNLIIRDGGFPGVVVRLGKAFAKVEATDATTLRCGGGASG
                     ILVSSTARDAAIAGMEFLRSIPGTVGGFVRMNGGAYGAEVKDILIDCDIVLRSGERQT
                     LPLADLGYTYRHSELPEGAVVIGATFRGRLGEPAAIQAEMDRISASREASQPLRSKTG
                     GSTFKNPDGHRAWQLVDEAGCRGFAVGGAQVSEKHTNFLINTGDATSADIEALGEEVR
                     RRVKDKSGIELQWEIQRVGKAK"
BASE COUNT          564 a         1205 c         1239 g          650 t
ORIGIN      
        1 gccaggttcg agttcgataa tctcattatt gtcgcgcggg ctggccgagg ccgagccgta
       61 gaagctctgc agccgctgcg cctggctacg gcgaatgcca aaggccgagg cgatgtcgtc
      121 ggtgatgtcg ctagcgccga agggcagcga cgccatctcg acgagcatgc cgccggcata
      181 gagcgacacc gtcgtcaccg ccgcgccgag ttcgaccagc gcgacgccaa ggtcgcgttc
      241 ctcatccgag aggcaggcaa gccccgccgc gatcggcgag gcgacgacgg cgttgacgtc
      301 gagatgcgcc tgacggaccg cggcctcgag gttccgcacc ggcgcgccgt cggccatgat
      361 gatatgaata tcgacgccca gccggtcggc gtggaggcca atcgggttct tcacgccgtt
      421 gagcccgtca agcgtgtaga gcgcgggctg cgcgtgcagg atcatgcggc cttcggggtc
      481 gatgccggcg cggcccgcgg cgagcaggtc gtcgatatct tcctgctcga tgcggtggcc
      541 gccgagttcg ctttcgattg gcgcgacatc gctcaaaagc ccgcccgccg aaaagctgac
      601 ccagacatcg tcgatgttga ggcccgcgat gcgttcggcc tgttcgatcg cctcgcgtac
      661 gatatgctcg gtctgttcca tgtcggcgac atagccgcgc tggactccgc ggctctcgcg
      721 ctgcccggtg ccaagcacgt gcagctggcc gtcggcggtc tgtcccgcga tcagcgcgca
      781 gaccttccac gacccgacat cgagcgcggt gatgatcttt tcaatgcgcg gcggcgccat
      841 ggcttatccc ttctccgccg aggcttcggc gaccgcatcg accgcggcgc gcgcatcgtc
      901 gagcttcgcg ggcgccacct gcccttcgtg cggcaggcgc agcacgaagc gcgcgggatc
      961 gcgcatgtcg aagcgcaata tgccgcgacc gagcaagcgg ttggcgccgt ccatatgcgc
     1021 gaatttggcg agcgcggcct ttgcctcggc ctcgccttcg ggaagcgaga gcgtttcgcc
     1081 gctgcggaaa cgcaggtccc agcggcgatt gccgacccag gtcgcgcccg cgagcaattc
     1141 cttgaggctg ctcgcctcgg tcagcaggcg gtcgagatcc tgcgagcgct ggttcgcttt
     1201 cggcccgatg acgagcggca ggtcgggcat cgtcgcgacg gtgacgggtt cgagcacgac
     1261 gcccttttcg tcgatcagcg acaggcgatt attatgctgc cagatcgccg ccggggtgcg
     1321 ctcgacaatg tcgacgacga gcgtgtcggg caggcggcgc gacacgcgtg catccttgat
     1381 ccagccatat ttcatcaggt ccgcgcggac atcctcgaga tcgaccgccg ccatcgagcg
     1441 gtccttctgc gcgagcgcga tgtcatagac cttgagccgg tcgatgcggt cggcaccgac
     1501 gacctcgacc ttccggacct gaaagcccgc gcggccgacg gcctgggcat attcctcgtg
     1561 gatcttggcg gtgacgccgg tcgcgtgcgc ggcgatcgcc acgaccgcgc acaggctgag
     1621 cccgatcgtc cagttggcga ccttttgcag ccgctgcggg ctgatcggca gcgcgttgat
     1681 gatcgcgttg aggcgcgact gctggatctt gcgccgcttt ttcggcgcac gcgcgggccg
     1741 tcttggcggt gctttcccgc gcttgatctg tgtcttgctc atgacaacgc ttcccccacg
     1801 atgcgttcga ccagttcggc ataatccatg ccgacggcgc gcccctgttc gggcacgagg
     1861 ctgagcgctg tcatccccgg ctgggtgttg acctcgagca ggaagatgcc cgcgaggcca
     1921 tgttcgtcgt cccagcgaaa gtccgagcgc gagacgccct tgcagccgag caggccgtgc
     1981 gcctgaatcg ccagatcctt catccgctgc gccacctcat cgggcacgtc ggcggggcag
     2041 acatgctcgg tcagcccttc ggtatatttg gcgtcatagt cgtagaagcc cgacttcacc
     2101 cgaagttcgg tgacagcaag cgccgtgccg ccgagcaccg cgacggtcag ttcgcggccc
     2161 ttgatgaaag gttcggcgag caggcggtcg aattcctgcc acggacccac ggcttcgcgc
     2221 gcgatcgggt tgccgtaatt gctgtcgtcc ttgacgatcg cgacgccgac cgacgagcct
     2281 tcgttgacgg gtttcagcac atagggacgc ggcaaggggt cgactgtaaa gaggctttcg
     2341 ctgtcgacca tcgtccccgt cggcatgggg ataccatgcg ggacaagcgc ctgtttcgtc
     2401 aattccttgt cgatcgcgat caccgaggtg acgaggccgc tgtgggtgta tttgagaccc
     2461 atcaggtcga gcatgccctg caccgtcccg tcttcgcccg ggacgccgtg gagcgcgttg
     2521 aacacgacat cgggcttcgc ctcggcaagc cgcagcgcga cgtcgcggtc catgtcgatg
     2581 cgtgtgaccc tgtgcccgcg gctttcgagc gcgtcggcga cgccattgcc gctcatcagc
     2641 gacacttcgc gctcggccga ccagccgccc atcaggacgg cgacatgcca cggaccccgg
     2701 ctcacttggc cttccccacg cgctgtatct cccattgcaa ttcgatgccg ctcttgtcct
     2761 tcacgcggcg gcggacttcc tcgccgagcg cctcgatatc ggcactcgtc gcgtcgccgg
     2821 tgttgatcag aaaattggtg tgtttctcgc tgacctgcgc gccgccgacg gcgaagccac
     2881 ggcagccggc ctcgtcgaca agctgccagg ccctgtgccc gtcgggattc ttgaaggtcg
     2941 agccgccggt tttcgagcgc agcggctgcg acgcctcgcg gctcgccgag atgcggtcca
     3001 tttcggcttg gatcgcggcg ggctcgccga ggcggccgcg gaaggtcgcg ccgatcacca
     3061 ccgcgccttc gggcaattcg ctgtggcgat aggtgtagcc gaggtcggcg agcggcagcg
     3121 tctggcgttc gcccgagcgc agcacgatgt cgcaatcgat gaggatatcc ttgacctcag
     3181 ccccataggc gccgccgttc atgcggacga aaccgccgac ggtgccgggg atcgagcgga
     3241 ggaattccat gccggcgatc gcggcgtcgc gcgcggtcga ggagacgagg atgcccgagg
     3301 cgccgccgcc gcagcgcagc gtcgtagcgt cggtggcttc gaccttcgcg aacgccttgc
     3361 cgaggcgaac gacgacgccg gggaagccgc cgtcccggat gatgaggttc gagccgagcc
     3421 cgagcgccat cacgggaatg gcggaatcga gatcgcgaag gaaatccgcg aggtcgtcgg
     3481 cgtctttcgg ttcgaacagc caatcggcgg ggccgcccga cttgaaccag acgagcgggg
     3541 cgagcggggc gttgggggtt agtttgccgc ggacggttgg gagggttgcg gtggtcataa
     3601 gccaatcctc cccacttgtg gggaggtggc agccgcgcag cggctgacgg agggggct
//
LOCUS       DATMSU010000543         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_27896, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000543 DATMSU010000000
VERSION     DATMSU010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27896"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VN034_14525"
     CDS             complement(<1..204)
                     /locus_tag="VN034_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWV61875.1"
                     /translation="MASERMLQFVGREQSYPDKRSAEERARDFREIAERYAAPDADAQ
                     AARCSQCGVPYCSVHCPLHNHIPD"
     gene            complement(364..1164)
                     /locus_tag="VN034_14530"
     CDS             complement(364..1164)
                     /locus_tag="VN034_14530"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWV61876.1"
                     /translation="MSIWLTAIILGIVEGLTEFLPVSSTGHLILATELLGYDAARWAI
                     FNIAIQPGAILAIVVLYRKLFWEMFTGFFRRDPQAIAFVRNLLLAFFPAVVLGLAFGD
                     YIELMLENAVIVAWALVIGGFAILLVERFAKTRDVGGVANVSARQSILVGLVQCIAMI
                     PGVSRSGATILGAMSFGVDRKTAAEFSFFLALPTLTGATVLQLFKHRDAITTNDLGLI
                     AVGALVSFVVAWAVIKAFLAVVTRYGFAPFAWYRIIVGIAALIWLGMR"
     gene            complement(1164..>1791)
                     /locus_tag="VN034_14535"
     CDS             complement(1164..>1791)
                     /locus_tag="VN034_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HWV61877.1"
                     /translation="AAGARALVHVSAIGADRESASAYGRSKGDGEAAVRAAFTVAAIL
                     RPSILFGREDHFINRFATMMRMAPVVPVIAPNAKFQPAYVGDVADAVVAALGSDAAGK
                     TYELGGPQVLTMGELLRWIADATGRSPLFVDVPDVIASALATGFGWAPGAPITKDQWL
                     MLQHDNVVAEGAEGFTQLGVTPASLGSVAEGWLVQYRRHGRFAELASR"
BASE COUNT          365 a          564 c          595 g          267 t
ORIGIN      
        1 gtcggggata tgattgtgga gcgggcaatg gaccgagcaa tagggcacgc cgcattgcga
       61 gcagcgcgcc gcctgcgcgt cggcgtcggg cgccgcgtag cgctcggcga tctcgcgaaa
      121 gtcgcgcgcg cgttcttcgg cggagcgctt gtccggatag gattgttcac gccccacaaa
      181 ttggagcatg cgttcggaag ccatgcagta tccccttcgt tgagaggcga aatgcatggc
      241 ccgaaacgcg ctgtcacggg cgaattgcgc gataggtcag ttatgcttac ctaaatcgga
      301 ggtcaaattt gccccaaaac cggttcgcgc aattatcttt cgtcaattag tctcgtttcg
      361 gatctatctc atgccgagcc agatcagcgc cgcgatcccg acgatgattc ggtaccaggc
      421 gaagggcgcg aagccatagc gtgtgaccac cgcgaggaag gccttgatca ccgcccaggc
      481 gacgacgaac gagacgagcg cgcccaccgc gatcaggccg aggtcgttgg tggtgatcgc
      541 gtcgcgatgc ttgaacagct ggagcaccgt cgcgccggtc agcgtcggga gcgcgaggaa
      601 aaagctgaac tcggccgcgg tcttgcggtc gaccccgaac gacatcgcgc cgaggatggt
      661 cgcgcccgag cggctgaccc ccgggatcat cgcgatgcac tggaccagcc cgacgaggat
      721 cgactggcgc gccgacacat tcgcgacccc cccgacatcg cgggtcttcg cgaaccgctc
      781 gacgagcaat atcgcaaaac ccccgatgac cagcgcccag gcgacgataa cggcattctc
      841 gagcatcaac tcgatatagt cgccgaaggc gagcccgagg acgaccgccg ggaaaaaggc
      901 gagcagcagg ttgcgaacaa aggcgatcgc ttgcgggtcg cggcggaaga agccggtgaa
      961 catctcccag aacagcttcc ggtacagcac gacgatcgcg aggatcgccc ccggctggat
     1021 cgcgatgttg aagatcgccc agcgcgcggc gtcatagccg agaagttccg tcgcaagaat
     1081 caggtgcccg gtcgaggaga cgggcaaaaa ctcggtcaga ccctcgacga tgccgaggat
     1141 gatcgcggtc agccagatag acattatcgc gaggcgagct cggcaaagcg cccgtggcgg
     1201 cgatactgga ccagccagcc ttcagcgacc gagccgagcg aggccggcgt gacgccaagc
     1261 tgcgtgaaac cttcggcacc ttcggcgacg acattgtcgt gctggagcat cagccactga
     1321 tccttggtga tcggcgcgcc gggggcccag ccaaagcccg tcgcgagcgc cgaggcgatg
     1381 acgtcgggga catcgacaaa caagggcgag cgtccggtcg catcggcgat ccagcggagc
     1441 aattcgccca tcgtcagcac ctgcggcccg ccgagctcat aggtcttgcc cgccgcatcg
     1501 ctgccgagcg ctgcgacgac cgcatcggcg acatcgccga cataggcggg ctggaatttg
     1561 gcattgggcg cgatcaccgg aaccaccggc gccatacgca tcatcgtggc aaagcggttg
     1621 atgaaatgat cctcgcgtcc gaagaggatc gaggggcgca ggatggcagc gacggtaaag
     1681 gcggcgcgca ccgcggcttc gccgtcgccc ttgctgcggc cataggccga ggcgctttcg
     1741 cggtcggcgc cgattgccga gacatggacg agcgcgcggg cgcccgccgc c
//
LOCUS       DATMSU010000544         5042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_179833,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000544 DATMSU010000000
VERSION     DATMSU010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179833"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VN034_14540"
     CDS             complement(<1..276)
                     /locus_tag="VN034_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWV61878.1"
                     /translation="MTFTAVPLTLYPDMFPGPLGHSMAGRALESGTWHCAPVQIRDFA
                     TDKHRTVDDTPAGGGAGMVLKADVLAAAIDHAMMAHPGLPILAMTPRG"
     gene            complement(273..1196)
                     /locus_tag="VN034_14545"
     CDS             complement(273..1196)
                     /locus_tag="VN034_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWV61879.1"
                     /translation="MPKLPPLAAVRTFEAAGRLGNFSRAAEELGLTQAAVSYQVRQLE
                     DRLGRALFVREKGRVRLSETGQRLLPAISGAFAAMGDAFAALGSDEAEVLTINAATSF
                     GGTWLSARIGGFQLAYPELAVRMAMSNELIDFDASNVDVAIRIGPGGWPGLRADFLMR
                     QYLAPMASPAFIEAHDIREPADLLRVQRLAPNDSWWAEWFALAGVDTPPVPSRRGIEL
                     DSQLQEASAVQAGFGVAMMTPLYWQAELASGRMVQPFDTLYVSARSGMWLVHRANRVG
                     VRKIERFREWLHAEIDKDRHLLPEALWQPPS"
     gene            1299..1502
                     /locus_tag="VN034_14550"
     CDS             1299..1502
                     /locus_tag="VN034_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61880.1"
                     /translation="MKDEHFMRVWNDGHDHFSADITRGLHWLAAPFRRLGEWIALPSA
                     PGEDSVFTRAAARCGIRPRRRAR"
     gene            complement(1581..3098)
                     /locus_tag="VN034_14555"
     CDS             complement(1581..3098)
                     /locus_tag="VN034_14555"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWV61881.1"
                     /translation="MRMKSGLVFVAILGVSAPLAAQDPAPPSLDRLAPEIDALFAEYQ
                     AEQHIPGLVYGVVKDGKLAYVKGIGVQNLTDKRPVTPDSLFRIASMTKAFTALAILKL
                     RDDGKLRLDDLAEQHIPEMKGWTYPTKDSPRIRIRDLLQHVGGFVTDDPWGDRQQVLP
                     QDEFTKMIAAGVPFSRVPQSSHEYSNFGYALLGRIVANASGMKYTDYVRQAILTPLGM
                     TASGFDVTKAPKARYAIGYRWENGHWSPEPEMADGAFNAMGGLQVSANDYAKWVAFLL
                     SAWPARDDVEAGPVKRSTVREVAQGLNFVSATNRIGASGASACKQAAAYGMGWRVAQD
                     CDLGLTLAHGGGYPGYGSHVMLMPDHGVGVFALANRTYAGPSAPAWDTAVAMEKAGLL
                     EPREVPVSPAVAQAYAAAKAAYAAGNLGPLQGRLAMNFLMDRSAGNWAAEFAELKAEV
                     GECPADEPLAPMGAMSTAFRLNCEKGKLDGILLLAPTTPSTVQALRFRIVPPDQN"
     gene            complement(3284..3748)
                     /locus_tag="VN034_14560"
     CDS             complement(3284..3748)
                     /locus_tag="VN034_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWV61882.1"
                     /translation="MSARIFETPMALLAAVGESFEPTGWVAIEQDRIDLFAKATDDHQ
                     WIHVDPERAKDGPFGGTIAHGYLTMSLVNRFLPELIEVRGVSMGVNVGTDNLRFLSPV
                     RSGSRIRGKGELVRAEEAKGGVQAVVRVIVEIDGEAKPACVVDTISRFYPET"
     gene            complement(3745..4527)
                     /locus_tag="VN034_14565"
     CDS             complement(3745..4527)
                     /locus_tag="VN034_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61883.1"
                     /translation="MARPAPEYPTPTGLLKGKTVLITAAAGSGIGGAVAQRAAEEGAT
                     LFLSDMHERRLGETADRIEGLGFARPGTLVCDVTNEEQVQALVAAAIAGMGHIDVLIN
                     NAGLGGAASVVEMTDDQWHKVLDVTLTSVFRITRAILPHMYERQKGAIVNNASVLGWR
                     AQKLQAHYAAAKAGVMAFTRCSALEAAEHGVRINAVSPSLAMHASLSKVTPPGLLETL
                     TEKEAFGRYAEPWEVANVMLFLASDLSSYMTGEVLAVSSQQA"
     gene            complement(4539..>5042)
                     /locus_tag="VN034_14570"
     CDS             complement(4539..>5042)
                     /locus_tag="VN034_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61884.1"
                     /translation="NILGAPGDGWTVAMGLLGFERGVSTLAQQMGFRNELDAVIDAAR
                     ANGEAKNPLIRQRIAKAEIGLKLMRYGALRMLTDAEDGKLNPSALTYKIQWATWRRDL
                     GELAMDVLGQAGETVAGHDYAFPALPNLYLYSRADTIYGGTNEIQRNIIAERALGLPR
                     EPRGDLK"
BASE COUNT          808 a         1666 c         1695 g          873 t
ORIGIN      
        1 gccgcgtggg gtcatcgcga ggatcggcag gcccggatgc gccatcattg catggtcgat
       61 ggcggcggcg agcacgtcgg ccttgagcac catgcccgct cccccgcccg cgggggtgtc
      121 atcgacggtg cggtgcttgt cggtcgcgaa atcgcggatc tgcaccggcg cgcaatgcca
      181 ggtcccgctt tcgagcgcgc gccccgccat gctgtgcccg agcggaccgg ggaacatgtc
      241 gggatagagg gtgagcggaa cggcggtgaa ggtcatgacg gcggctgcca tagcgcctcg
      301 gggagcaggt ggcgatcctt gtcgatttcg gcgtgcaacc attcgcgaaa gcgttcgatt
      361 ttgcgcaccc ccacgcggtt ggcgcggtgg acgagccaca tccccgatct cgccgagaca
      421 tagagagtgt cgaaaggctg caccatgcgc ccgctcgcca gttcggcctg ccaatagagc
      481 ggggtcatca tcgcgacgcc gaagcccgcc tgtaccgcgc tcgcttcctg caactggctg
      541 tcgagctcga tcccgcggcg cgacggcacg ggcggtgtgt cgacgccggc cagggcgaac
      601 cattcggccc accagctgtc gttcggcgcg agccgctgga cgcgcagcag gtcggcgggc
      661 tcgcgaatat catgcgcctc gataaaggcg ggcgaggcca tcggcgcgag atattggcgc
      721 atcagaaaat cggcgcgcag ccccggccac cctcccgggc cgatgcggat cgcgacatcg
      781 acgttcgagg cgtcgaagtc gatcaactcg ttgctcatcg ccatgcgcac cgcgagttcg
      841 ggataggcga gctggaagcc gccgatgcgc gcgctgagcc aggtgccgcc gaagctcgtc
      901 gcggcgttga tcgtcagcac ttcggcctcg tcgctaccca aggcggcgaa ggcgtcgccc
      961 atcgcggcga aggcgcccga gatcgcgggg agcagccgct gccccgtttc ggaaaggcgc
     1021 acccgcccct tttcgcgcac gaagagcgcg cggccgagcc ggtcctccaa ttggcggacc
     1081 tgatagctca ccgccgcctg cgtcaacccg agctcctcgg cggcgcgcga gaaattcccc
     1141 agacgtccgg cggcttcgaa cgtgcgtacg gcggcgaggg gcggcagctt gggcatgacg
     1201 catcataagc ccagcttatg atatggtcca cgccttttgt tggcggtgcg gcgcctgaag
     1261 gtgcatctag gcatcactca agcgaaagga gatgagcgat gaaagacgag catttcatgc
     1321 gcgtttggaa tgacggtcat gatcatttca gcgccgatat cacccgcggc cttcactggc
     1381 tcgcggcccc gttccgccgc ctcggcgaat ggatcgcctt gccgagtgcg cccggcgaag
     1441 acagcgtctt cacccgcgcc gccgcacgct gcggcatccg cccgcgccgc cgcgcgcgat
     1501 aattccctgc ctcccctctc aaaaggtcca gggcgtaacg ggacggggtc gtcccttgca
     1561 aagaccccgt cccgttttca tcagttctga tcgggcggga cgatccggaa acgcagcgcc
     1621 tggaccgtcg atggcgtcgt cggggcgagt agcaatatcc cgtcgagctt gcccttttcg
     1681 cagttgagcc ggaaggcggt cgacatcgcg cccatcgggg cgagcggttc gtcggcggga
     1741 cattcgccga cctctgcctt cagttccgcg aattcggccg cccaattgcc ggccgagcga
     1801 tccatcagga aattcatcgc gagcctgccc tgaagcgggc cgagattgcc cgcggcataa
     1861 gcggcctttg ccgcggcata agcctgtgcc accgcgggtg acaccggcac ttcgcgcggt
     1921 tcgagcagtc ccgccttctc catcgctacc gccgtatccc acgcgggcgc cgacggtccg
     1981 gcgtaggtgc ggttcgccag cgcgaagacg ccgacgccat gatcgggcat cagcatcaca
     2041 tggctgccat aaccgggata gccgccgccg tgcgcgagcg tcagcccgag gtcgcaatcc
     2101 tgcgcgacgc gccagcccat gccataggcc gcagcctgct tgcacgcgct cgccccgctg
     2161 gcgccgatgc gattggtcgc cgacacgaaa ttgagcccct gcgccacctc gcgcaccgtc
     2221 gaccgcttca ccgggccggc ttccacatcg tcgcgcgcgg gccaggcgga gagtaggaag
     2281 gcgacccatt tcgcataatc gttcgcgctg acctgcagtc cgcccatcgc gttgaacgcg
     2341 ccgtcggcca tctcgggctc gggcgaccag tgaccgtttt cccagcgata gccgatcgca
     2401 taacgcgctt tcggcgcctt ggtgacgtcg aagccgctcg ccgtcatgcc gagcggggtc
     2461 aggatcgcct gccggacata gtcggtatat ttcatgcccg acgcattggc gacgatgcgg
     2521 ccgagcagcg catagccgaa gttcgaatat tcgtggctgc tctgcggcac gcggctgaac
     2581 ggcacgcccg cggcgatcat cttggtgaat tcgtcctgcg gcaagacctg ctgccggtcg
     2641 ccccaagggt cgtcggtgac gaagccgccg acatgctgga gcaggtcgcg gatacggatg
     2701 cgcgggctgt ccttggtcgg gtaggtccag cctttcattt cggggatatg ctgctcggcg
     2761 agatcgtcga ggcggagctt gccgtcgtcg cgcagcttga ggatcgcgag cgcggtgaac
     2821 gccttggtca tcgaggcgat gcggaacagg ctgtcggggg tgaccgggcg cttgtcggtc
     2881 aggttctgga cgccgatgcc cttcacatag gcgagcttgc cgtccttgac gacgccatag
     2941 acgaggccgg ggatatgctg ctcggcctga tattccgcga acagcgcgtc gatttcgggg
     3001 gcgagccggt cgaggcttgg gggcgccggg tcctgggcgg caaggggtgc ggagacgcca
     3061 aggatggcga caaaaacgag acccgacttc attcgcatgg cgatcctccc ctgacgagca
     3121 cgcgcgaggc taacagcggc cgggggaggg gacaatccat cgttcgtccg gtggtagacg
     3181 gcggccaatg gccgtcagga gacgttggca aacatgagcc agcccctctt cttcagagga
     3241 ggggttaggg catcgtccgc tccccacccc agagccgcct tcgttacgtc tccggataga
     3301 agcggctgat cgtatcaacg acgcacgcgg gcttcgcttc gccgtcgatc tctacgatca
     3361 cgcgaaccac ggcctggacg ccgcctttgg cttcctctgc cctcaccagt tcgcctttgc
     3421 cgcggatccg actgcccgaa cgaaccgggc tcaggaagcg caaattgtcg gtgccgacat
     3481 tgacccccat cgacaccccg cgcacctcga tcagttcggg gaggaagcgg ttgacgaggc
     3541 tcatcgtcag atagccgtgc gcgatcgtgc cgccgaacgg accgtccttc gcgcgctcgg
     3601 gatcgacgtg gatccattgg tggtcgtcgg tggccttggc gaacaggtcg atgcgatcct
     3661 gctcgatcgc gacccatccc gtcggctcga agctctcacc caccgcggcg agcagcgcca
     3721 tcggggtttc gaagatgcgc gcgctcatgc ctgctggctc gacaccgcga gcacttcgcc
     3781 cgtcatgtag gaggaaaggt cgctggcgag gaagagcatc acgttcgcga cctcccaagg
     3841 ctcggcatag cggccgaagg cttccttctc ggtcagcgtc tcgagcagcc ccggcggggt
     3901 caccttggaa agcgaggcgt gcatcgcgag gctgggcgaa accgcgttga tgcggacgcc
     3961 gtgctcggcg gcttcgagcg ccgagcagcg cgtgaaggcc atcacccccg ccttggccgc
     4021 ggcataatgc gcctgcagct tctgcgcgcg ccagccgagg accgaggcgt tgttgacgat
     4081 tgcgcctttc tggcgctcgt acatgtgcgg caggatcgcg cgcgtaatgc ggaagacgct
     4141 ggtgagtgtc acatcgagca ccttgtgcca ctggtcgtcg gtcatctcga cgaccgaagc
     4201 cgcgccgccg aggccggcat tgttgatgag gacatcgata tggcccatgc ccgcgatcgc
     4261 agcggcgacg agcgcctgca cctgttcttc gttggtgacg tcgcagacga gcgtgccggg
     4321 gcgtgcgaag cccaatccct cgatccggtc ggcggtctcg cctagacggc gttcgtgcat
     4381 gtcagagagg aagagcgttg cgccttcctc ggccgcgcgc tgcgcgaccg cgccgccgat
     4441 gccgctgccc gcggcggcgg tgatcagcac ggtcttgccc ttcagaaggc cggtcggggt
     4501 cggatattcg ggggcgggtc gtgccacgct tggttccttt atttgaggtc gccgcgcggt
     4561 tcgcggggta gccccagcgc gcgctcggcg atgatgttgc gctgaatctc gttcgtgccg
     4621 ccgtaaattg tgtcggcgcg gctgtagagg tacaggttgg gaagcgcggg gaaggcatag
     4681 tcgtgccccg cgacggtctc gcccgcctgt cccagaacgt ccatcgcgag ctcgccgaga
     4741 tcgcggcgcc acgtcgccca ctggatcttg taggtgagcg ccgacgggtt gagcttgccg
     4801 tcctcggcgt cggtgagcat gcgcagcgcg ccatagcgca tcagcttcaa tccgatttcg
     4861 gccttggcga tgcgctggcg gatgagcggg ttcttcgcct caccattggc gcgcgccgcg
     4921 tcgatcaccg cgtcgagttc gttgcgaaag cccatttgct gcgcaagcgt cgagacgccg
     4981 cgctcgaagc cgagcagccc catcgcgacc gtccagccat cgccgggcgc gccgaggatg
     5041 tt
//
LOCUS       DATMSU010000545         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_132635,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000545 DATMSU010000000
VERSION     DATMSU010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132635"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VN034_14575"
     CDS             complement(<1..224)
                     /locus_tag="VN034_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWV61885.1"
                     /translation="MTDTPSFDTIKLDIADHVATITLNRPDRLNSMPPAMADDIREAL
                     DHLPGLGARALLITGEGRGFCSGADLAGDRS"
     gene            535..1368
                     /locus_tag="VN034_14580"
     CDS             535..1368
                     /locus_tag="VN034_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HWV61886.1"
                     /translation="MTSTPPPPPARANTSLTNLQVSEDFVGRAGVMQYSVLRSTGGAT
                     QREAALNSSALVRYDASTQSYTVVGINLPQSIFAAANRQSGESTPVISVYEKVNGTKR
                     ENLALFNPGAANTELALTYTSYGALQTIVDKGASLDVDTAFFTFGVQTAASDMPRTGT
                     SDYRTKIDGQFSDAGGVYALSGPSTFSANFGAGTFAFTMDLTGQNVVDGSRKSLGAHS
                     IDGSISYGNQFSGASSRDGTYSSNLGGYFYGPAAAEMGGSFSLVGGGGVGAGAVVGKK
                     E"
     gene            complement(1473..>2150)
                     /locus_tag="VN034_14585"
     CDS             complement(1473..>2150)
                     /locus_tag="VN034_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61887.1"
                     /translation="GLAPAPRRTHDRAMRTLLLAAFLIGAPAAAQDSSFVGEYSLAAG
                     PDVGGGLLIRSDGRFQYMLAAGALDERAEGRWEMRDEMICLTTDPKPVPPAMEKGPLI
                     EVEGAIPTLLVTWPNGEGVPGVDFTIGFDNGEPAEDYTQYYGWTMPEDDAREPRWVEL
                     REPIYDITAPRYELTEADDGKLRVIIVPNDIGVVDFDGACAEKTERGLTLHRAEGDMR
                     FARLKSE"
BASE COUNT          370 a          645 c          730 g          405 t
ORIGIN      
        1 gaccggtcgc cggcgaggtc ggcgcccgaa cagaagccgc ggccttcgcc ggtgatcagc
       61 agcgcgcgcg cgccgaggcc gggcagatgg tcgagcgcct cgcgaatatc gtcggccatc
      121 gcgggcggca tcgaattgag ccggtcgggg cggttgagcg tgatcgtcgc gacatggtcg
      181 gcgatgtcga gcttgatggt gtcaaagctg ggagtgtcgg tcatggggaa gaatcctctc
      241 gcgggtgctt tacctttacg ttcacgtaaa gatgtgacgc atttgggaag gggatgcaag
      301 gggcttggac gacaaggccg gacgatctcg cgcgcccgaa cggccgcgaa ggcgggtgtc
      361 gtcccaagaa cgtccgtgcc ccggattcgg cgccttatcg gccttgacgg gattggggat
      421 cgcggcaaga tagcgggatt attcgggggg aaatttatga attaccagcg gtttgtgctt
      481 gtcggcttcg cttgcaccgc tttgtcggcc tgcggtggcg gagggggcgg gggcttgacc
      541 tcgacgccgc cgccgcctcc cgcccgagcc aatacttcgc tcaccaattt gcaggtcagc
      601 gaggatttcg tcggccgcgc tggggttatg cagtatagtg ttctgcgttc gaccggaggc
      661 gcgacgcagc gtgaagcagc cctgaattcg agcgccctgg tgcgatatga cgcgagtacg
      721 caatcctata cggtggtggg tatcaacctg cctcagtcca tattcgcggc agcgaacaga
      781 cagtccggcg aaagcactcc ggtcatttcg gtttacgaaa aggtgaatgg taccaagcgc
      841 gaaaatctcg ccctgttcaa ccctggcgcc gccaacacgg agcttgcgtt aacatatacg
      901 agctacgggg cgctgcagac gatcgtcgac aagggcgcga gcctcgatgt cgacacagcg
      961 ttctttacct tcggggtgca gacggccgcc agcgatatgc cacggaccgg gacgtccgat
     1021 tatcgtacga agatcgacgg gcagttttcg gatgcaggcg gcgtatacgc cctttccggc
     1081 cccagcacct tctccgcaaa tttcggggcg ggaacattcg ccttcaccat ggacctgaca
     1141 ggacagaatg tcgtcgacgg cagccggaaa agccttggtg cgcatagtat cgacggatcg
     1201 atcagctatg gcaaccagtt cagcggcgca agcagccgtg atggcaccta ttcttccaat
     1261 ctcggcggct atttctacgg acccgccgca gcggaaatgg gcggaagctt ttcgctcgtg
     1321 gggggaggcg gcgtcggggc tggcgcggtc gtcgggaaaa aggagtgaca ccttcgccgg
     1381 gcatttcagc ggtgtcgatg tcgttggtcc cggtggatat tccggccgtg cagcgtgcgg
     1441 cgaagggctg ctagggtcct aatcccggcc cgtcattcgc ttttcagccg cgcgaaccgc
     1501 atatcgccct cggcgcggtg cagcgtcagc ccgcgctcgg tcttctccgc gcaggcgccg
     1561 tcgaaatcga cgacgccgat atcgttgggc acgatgatca cgcgcagctt gccgtcgtcg
     1621 gcttcggtga gctcgtagcg cggcgcggtg atgtcgtaga ttggctcgcg cagctcgacc
     1681 cagcgcggct ctcgcgcgtc atcctcgggc atcgtccagc cataatattg ggtataatcc
     1741 tcggccggtt cgccattgtc gaagccgatc gtgaaatcga ctcccggcac gccttcgccg
     1801 ttcggccagg tgacgaggag ggtggggatg gcgccttcga cctcgatcag cggccccttc
     1861 tccatcgccg gcggcaccgg cttgggatcg gtggtgaggc agatcatctc gtcccgcatt
     1921 tcccagcggc cctcggcacg ctcgtcgagc gcacccgcgg cgagcatata ttggaagcgg
     1981 ccgtcgctgc ggatcagcag cccgcccccg acgtcgggtc ccgcggcgag gctgtattcg
     2041 ccgacgaagg atgaatcctg agcggcggcg ggggcgccga tcagaaaggc ggcgaggagc
     2101 agcgttcgca tggcgcgatc atgcgttcgc cgcggcgcgg gcgccagccc
//
LOCUS       DATMSU010000546         8785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_75683, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000546 DATMSU010000000
VERSION     DATMSU010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8785
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75683"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VN034_14590"
     CDS             <1..991
                     /locus_tag="VN034_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWV61888.1"
                     /translation="RAPSFFVLDVPRSDAAGFRAIVTEADPKAEINMIPALRGSVTEF
                     RGQRVDELAELPEGAWVLRGDRGLTYSPTLPNGSELVGGAWWAADYTGPPLVSVEQEV
                     ATSLGLGLGDTLSVNVLGVEVQAKVASFRTVNWDNFGLNYVLVFSPGTFDAAPHNMVA
                     TVAVGPEAETALSRSIPRAFPSASLIAVRDVVSQVTTLLTQMSQAIAAAASIAILAGI
                     AVLIGAIAASRERRVYDSVILKLLGATRGQILGAQGMEYAVLAAILAVLALGLGLAGA
                     WYVVTQLFEFDFAPDPLIVGLTLLGGAGLSFLIGIAGSWPLLSAKPAQALRSL"
     gene            complement(1093..1854)
                     /locus_tag="VN034_14595"
     CDS             complement(1093..1854)
                     /locus_tag="VN034_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWV61889.1"
                     /translation="MIDAPHLFGFAPLTLVGAAAMTFGAAYVRGLTGFGMAIILVPLL
                     GILIPPGEAVVMGILLQLLIGPVGIGHIIADADRETALPIGLAAMATTPIGMYVLDLT
                     PADIARLLITLAAVGAFVAVLLPKKPEGHRPGRLAVAGTGITSGILTGFAAMPGPPVV
                     PFYLRRRIDPKVARASMLMIFFMTAIAGTLASLWLGIATWRLFLMAVLLFLPMWLGNY
                     VGARHFGRVPPHIWQAMVALVLGVAAVSAVVRLLN"
     gene            complement(1851..2708)
                     /locus_tag="VN034_14600"
     CDS             complement(1851..2708)
                     /locus_tag="VN034_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWV61890.1"
                     /translation="MSARPRRRWLRWAALLALLGLLLLAKGYWNATRDPVVRTATVAV
                     ADWPAGQAPLKLLLISDTHVAGPDMPPERLSRIVGQLNRLKPDVVLVAGDLVSEKRIA
                     THIYTADEVVAPLAGLKAPLGVVVAPGNHDHWFGPDALRGALERHGLRVLQNEAVKLG
                     PLIVGGVDDDYSGHDDVPATLAAMDRLGPGIPLILSHSPDIAPAVKRPVAAILAGHTH
                     CGQIRFPFIGALTYVSQYGDRFACGDISDGGQRVFVGAGLGTSLLPLRYLTPPDAWLI
                     TLKPKDSRP"
     gene            complement(2705..4708)
                     /gene="thrS"
                     /locus_tag="VN034_14605"
     CDS             complement(2705..4708)
                     /gene="thrS"
                     /locus_tag="VN034_14605"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWV61891.1"
                     /translation="MSEMLKISLPDGSVRDMPAGSTPADVAAAIGPGLAKAAIAARVN
                     GELRDIMRPFEGDTDLALVTSRDEADALELARHDYAHVLAEAVQKLFPGTQITFGPST
                     GDGFYYDFAPSPERGPFRDDELPLIEEEMRRIIAADLPLTREVWERDKLIAKWAAEGE
                     TFKAEWAAELPEGEELTVYRSGDGWMDMCRGPHLASTGKLDPAAFKLTRVSGAYWRGD
                     QKNAQLSRIYGTGWLNKKQLAEHLVRLEEAAKRDHRKIGREMDLFHLQEEAHGSVFWH
                     PKGYRIYRELEAYMRRAIDGSGYQEVKTPQVMDAKQWEQSGHWGKYRENMFVIPDEIP
                     NIEDEGPIVSDDAEWMALKPMNCPAHVLIFRQGMKSYRDLPLRMAEMGCCHRNEPHGA
                     LHGIMRVRQFTQDDGHIFCREDQIVEEVRAFCELLDRVYRQLGFEKYAVKLALRPEKR
                     FGTEEMWDKAEAELREAVARAGMANDDYGWEELPGEGAFYAPKLEFHLTDAIGRTWQC
                     GTIQSDRVMPERLDASYIGEDGERHRPVMLHRAILGTYERFIGILIEHFAGRFPTWLA
                     PVQAVVATIVSDADDYAKDAVAQLTAAGIRADSDLRNEKINYKVREHSLAKVPYLLVV
                     GKREAEEGTVAIRTLGQDGQRIMPLAEAIAMLKAEATPPDLKD"
     gene            4999..5376
                     /locus_tag="VN034_14610"
     CDS             4999..5376
                     /locus_tag="VN034_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61892.1"
                     /translation="MSDAPTHSIAAILPCSDLDASTAFYEVLGLKVVSDHGTYRLLAD
                     GKGWQLHLTSESGPTWPERDQNPFGLYLYTERVAELAEGVRDRILEREKAPTHKPWGM
                     YEFALSDPDETLVRVGWPSELIG"
     gene            5449..5712
                     /locus_tag="VN034_14615"
     CDS             5449..5712
                     /locus_tag="VN034_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6356 family protein"
                     /protein_id="HWV61893.1"
                     /translation="MIKSLKRLFVDHPKSVDENYIEHFGVASKFGVTMIYGGLCALVH
                     AIVPGWCITTGSDTIQRLNHIMVEKRRAKGQAVTQMSTIDWVI"
     gene            5846..6607
                     /locus_tag="VN034_14620"
     CDS             5846..6607
                     /locus_tag="VN034_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWV61894.1"
                     /translation="MNELAPDYLERSGRPRLAFRHTPGVGPTIVFLPGYMSDMAGGKA
                     TALFDWAVAEGHACLLLDYAGCGLSDGLFADQTLLDWRGDILDLIDAKAEGPVVIVGS
                     SMGGWLMLLTALALVQRDGPGRVAGLVGIAAAPDFTDWGFTPEEKAIIQAEGALVEET
                     PYSDQPYMTTRGFFQSGEANRLLDSDIPLACPVRLLHGEEDGDVPPSISLRLSAALSS
                     EDVQVTLVKGGDHRLSRDTDIALLIDTVARLATQI"
     gene            6620..7057
                     /locus_tag="VN034_14625"
     CDS             6620..7057
                     /locus_tag="VN034_14625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWV61895.1"
                     /translation="MARSDFRFHVTKRVRYAEIDAQRVVFNSRYLEYFDIGITEYWRA
                     AGVYDRWPDHSSPEFHVARAEVDYKVPILLDEEIDICVRASRVGKSSMTFLFELHGAG
                     KDDLRASGQLINVHVAEAQGAPAPVPDEFLFLFEAFEARPLRA"
     gene            7068..7505
                     /locus_tag="VN034_14630"
     CDS             7068..7505
                     /locus_tag="VN034_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61896.1"
                     /translation="MTKYLHTMIRVSDPDATVTFFKLIGLEETRRFDNEAGRFTLIFL
                     AAPGQGDVAEVELTYNWPPADGSPAEEYTGGRNFGHLAYRVDDIYATCQRLMDAGVTI
                     NRPPRDGHMAFVRSPDGISVELLQEGTLDPAEPWASMPNTGAW"
     gene            7517..7924
                     /locus_tag="VN034_14635"
     CDS             7517..7924
                     /locus_tag="VN034_14635"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWV61897.1"
                     /translation="MAGLLINIDVPDLAAGERFYTAALGLSAARRLGDDIVELTGCEI
                     PIYLIAKPAGSMIGPDGGDFRRYNRHWTPVHPDFTVDDLGDAIARALDAGAVREGETL
                     DLPYGRQAMFADPFGNGFCLIEFNERGYDALLT"
     gene            7993..8721
                     /gene="gloB"
                     /locus_tag="VN034_14640"
     CDS             7993..8721
                     /gene="gloB"
                     /locus_tag="VN034_14640"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HWV61898.1"
                     /translation="MAALDIVRIPVLSDNYVWLVHDPASEATMVVDPAVAEPVLEAAA
                     ARGWTITDIWNTHWHPDHTGGNAAIKEATGCTITGPAAEFARIPTLDVHVKGGDQVRL
                     GNHVANVWDVPAHTAGHIAYHFAEDAAIFVGDTMFAMGCGRLFEGTAEQMFDNMRKLD
                     ALDDATRVYCAHEYTLSNARFAVTVDPDNEALAARLAAVEAARAEGEATVPTSIGEER
                     ATNPFFRAPDAAELARIRALKDAA"
BASE COUNT         1506 a         2957 c         2860 g         1462 t
ORIGIN      
        1 gcgcgcgccg agcttcttcg tactcgatgt gccgcgcagc gacgccgcag gcttccgcgc
       61 gatcgtcacc gaggccgatc ccaaggccga aatcaacatg atccccgcgc tgcgcggcag
      121 cgtcaccgaa tttcgcggcc agcgcgtcga cgagctcgcc gaattgcccg agggcgcatg
      181 ggtgctgcgc ggcgaccgcg ggctgactta tagcccgacc ctgccgaacg gcagcgaact
      241 ggtcggcggc gcatggtggg cggccgatta cacgggaccg ccgctggtca gtgtcgaaca
      301 ggaggtcgcg acctcgctcg ggctggggct tggcgacacg ctgtcggtca atgtgctggg
      361 ggtcgaggtg caggccaagg tcgcctcgtt ccgcaccgtc aactgggaca atttcgggct
      421 caactatgtg ctcgtcttct cacccggcac cttcgacgcc gcgccgcaca atatggtcgc
      481 gaccgtcgcg gtcggccccg aagccgaaac cgcgctgtcg cgcagcatcc cgcgcgcctt
      541 tccttccgcc tcgctgatcg cggtgcgcga tgtcgtgagc caggtcacca ccctgcttac
      601 ccagatgagc caggcgatcg ctgccgcagc gagcatcgcg atcctcgccg ggatcgcggt
      661 gctgatcggc gcgatcgcgg cgagccgcga gcggcgcgtc tacgacagcg tcatcctgaa
      721 gctgctcggc gcgacgcggg gccagatatt gggcgcgcag ggcatggaat atgcggtgct
      781 cgccgcgatc ctcgcggtcc tcgcgctggg attggggctc gcgggcgcct ggtatgtggt
      841 gacgcaattg ttcgagttcg acttcgcgcc cgacccgctg atcgtcggac tgaccttgct
      901 cggcggcgcg ggtctgtcgt tcctgatcgg catcgcggga agctggcctt tgctctcggc
      961 caagcccgcg caggcgctgc gcagccttta gcacattcct tcccgctcgt gtcgagcgaa
     1021 gtcgagacac ccagaaggca tatgccgccg atgggcatct cgacttcgct cgatgcgaac
     1081 ggggaaggaa agtcagttca agaggcgcac caccgccgaa accgccgcga cgccgagcac
     1141 aagcgcgacc atcgcttgcc agatgtgcgg cggcacgcgg ccgaaatggc gcgcgccgac
     1201 ataattgccg agccacatcg gcaggaacag cagcaccgcc atcaggaaca gccgccaggt
     1261 cgcgatgccg agccacagcg acgcgagcgt tcccgcgatc gcggtcatga aaaagatcat
     1321 cagcatcgac gcgcgcgcga ccttgggatc gatccggcgt cgcaggtaaa agggcacgac
     1381 cggcggcccc ggcatcgcgg cgaagccggt gaggatgccc gacgtgatgc ccgttccggc
     1441 gacggcaagc cgtccggggc gatgcccctc gggctttttc ggcagcagga cggcgacgaa
     1501 ggcgccgacg gcggcgagcg tgatcagcag ccgcgcaatg tcggcgggcg tcaggtcgag
     1561 cacgtacatg ccgatcggtg tcgtcgccat cgccgcgagc ccgatcggaa gcgcggtctc
     1621 gcggtcggcg tcggcgatga tatggccgat cccgacaggg ccgatcagca gctgcaacaa
     1681 tatgcccatc accaccgcct cgcccggcgg tatcaggatg ccgagcagcg gcacgaggat
     1741 gatcgccatg ccgaacccgg tcagcccgcg cacataggcg gcgccaaagg tcatcgcggc
     1801 tgcgccgacg agcgtcagcg gcgcgaaacc gaaaaggtgc ggggcatcga tcatgggcgc
     1861 gaatctttcg gtttcagcgt gatcaaccac gcgtcgggcg gcgtcagata acgaagcggc
     1921 aagaggctgg tcccgagccc cgcgccgacg aagacgcgct ggcccccgtc gcttatgtcg
     1981 ccgcaggcga agcggtcgcc gtattgcgaa acgtaggtca gcgcgccgat gaaggggaag
     2041 cggatctggc cgcaatgcgt atgcccggcg aggatcgccg cgaccgggcg cttcaccgcc
     2101 ggcgcgatgt cggggctgtg cgacaagatc aggggaatgc cgggacccag ccggtccatc
     2161 gcggcaagcg tcgcgggcac atcgtcatgt cccgaataat cgtcatcgac cccgccgacg
     2221 atcagcggcc cgagcttgac cgcctcattc tgcaagacgc gaagcccgtg ccgttcaagc
     2281 gcgccgcgca gcgcgtcggg tccgaaccag tgatcgtgat tgcccggcgc gaccacgacg
     2341 ccgagcggcg ctttcagccc ggcgagcggc gccaccacct cgtcggcggt ataaatgtgg
     2401 gtcgcgatcc gcttctcgct aacgagatcg cccgcgacga gcacgacgtc gggcttcagc
     2461 cggttgagct ggccgacgat gcgcgacagc cgctcgggcg gcatgtcggg gccggcgaca
     2521 tgcgtgtccg aaatcagcaa gagcttcagc ggcgcctgac ccgcgggcca atcggcgacc
     2581 gcgaccgtcg cggtgcggac gaccggatcg cgcgtcgcat tccaatagcc cttggccagc
     2641 agtagaaggc cgagcagcgc cagcagcgct gcccagcgca gccagcgccg ccgcggcctc
     2701 gcgctcaatc cttgaggtcc ggcggggtcg cttcggcctt cagcatcgcg atcgcttcgg
     2761 cgagcggcat gatgcgctgg ccgtcctgtc cgagggtgcg gatcgcgact gtgccctcct
     2821 cggcttcgcg cttgccgacg acgagcagat aggggacctt cgcaaggctg tgttcgcgga
     2881 ccttgtaatt gatcttctcg ttgcgaaggt cgctgtcggc gcggatgccc gccgcggtga
     2941 gctgcgccac cgcgtccttg gcataatcgt cggcgtcgct gacgatcgtc gcaaccaccg
     3001 cctgcaccgg cgcgagccag gtcgggaagc ggccggcgaa atgctcgatc aaaatgccga
     3061 tgaagcgttc gtaagtaccg aggatcgcgc ggtggagcat taccgggcgg tggcgctcgc
     3121 catcctcgcc gatatagctc gcgtcgagcc gctcgggcat cacgcggtcg gactggatcg
     3181 tcccgcactg ccacgtccgg ccgatcgcgt cggtcaggtg gaattcgagc ttgggcgcat
     3241 agaaagcgcc ctcgcccggc aattcctccc agccataatc gtcgttcgcc atgcccgcgc
     3301 gcgcgacggc ttcgcgcagc tcggcctcgg ccttgtccca catttcttcg gtgccgaagc
     3361 gcttttcggg gcgcagcgcg agcttcacgg catatttttc gaacccgagc tggcgataga
     3421 cacggtcgag cagttcgcaa aaggcgcgca cttcctcgac gatctggtcc tcgcggcaga
     3481 agatatggcc gtcgtcctgc gtgaactggc gcacgcgcat gatgccgtgc agcgcgccgt
     3541 gcggctcgtt acggtggcag cagcccattt cggccatgcg cagcggcagg tcgcgatacg
     3601 acttcatccc ctggcgaaag atcagaacat gcgccgggca gttcatcggc ttcaatgcca
     3661 tccactcggc atcgtcggag acgatcgggc cctcatcctc gatattcggg atttcgtcgg
     3721 ggatgacgaa catattttcg cgatatttgc cccagtggcc cgactgttcc cactgcttgg
     3781 cgtccatcac ctgcggcgtt ttgacctcct gatagcccga gccgtcgatc gcgcggcgca
     3841 tataggcttc gagctcgcga tagatgcgat agcccttggg gtgccagaag acgctgccat
     3901 gcgcctcttc ctgcaaatgg aacaggtcca tctcgcgccc gatcttgcgg tggtcgcgct
     3961 tcgcggcctc ttcgagccgg acaagatgct cggcaagctg cttcttgttg agccagccgg
     4021 tgccatagat gcgcgaaagc tgcgcatttt tctggtcacc gcgccaatag gcgcccgaga
     4081 cgcgcgtcag cttgaacgcg gcgggatcga gcttgcccgt cgaggcgagg tgcggcccgc
     4141 ggcacatgtc catccagccg tcgccgctgc gataaacggt gagttcctcg ccctcaggca
     4201 gttcggccgc ccattcggcc ttgaacgtct caccctcggc cgcccatttg gcgatgagct
     4261 tgtcacgctc ccacacctcg cgcgtgaggg gaaggtcggc ggcgatgatg cggcgcatct
     4321 cctcctcgat cagcggcagt tcgtcgtcgc ggaacgggcc gcgctcgggg ctcggcgcga
     4381 agtcgtaata gaagccgtcg cccgtcgacg ggccgaaggt gatctgcgtc cccggaaaca
     4441 gcttctgcac cgcctcggcg agcacatgcg catagtcgtg gcgcgccagt tcgagcgcgt
     4501 cggcttcgtc gcggctcgtg accagcgcga ggtcggtatc gccttcgaac ggacgcatga
     4561 tgtcgcgcag ctcgccgttg acgcgcgccg cgatcgccgc cttggcgagg ccggggccga
     4621 tcgccgccgc gacatccgcc ggggtcgaac ctgcgggcat gtcacgcacc gaaccgtcgg
     4681 gaaggctgat tttcagcatc tcactcatcg aaatccgtct ttcatcgctt tggtccgcgc
     4741 catggcacaa ggcggcgcgc accggaagag gccgcgtcca gctttcggaa attcgggtgg
     4801 aaggacgccg cgccacccga acccgggtgg cagccggcgc gcgttcaggc cgcggccgac
     4861 cgcccccggc atgcgggggt agtggtggtc tgggtcgtga tcggcgtcag cgtcatgccc
     4921 tccacatagg cggcgcgaaa gcctttgtca tcacccgtcc gaaacctatg ctgaccacct
     4981 gccttcagga gttcgaccat gtccgacgca ccgacgcaca gcatcgccgc catccttccg
     5041 tgcagcgacc tcgacgccag caccgccttc tatgaagtgc tgggactgaa ggtcgtgagc
     5101 gaccatggca cctatcgcct gctcgccgac ggcaagggct ggcagctgca tctgacgagc
     5161 gaatccggcc cgacctggcc cgaacgcgat cagaatcctt tcggtctcta cctctatacc
     5221 gagcgtgtgg ccgaactcgc cgaaggggtg cgagaccgca tcctcgaacg cgaaaaggcg
     5281 ccgacgcaca agccgtgggg catgtatgaa ttcgcgctgt cggaccccga cgagacgctg
     5341 gtgcgcgtcg gctggccgag cgagttgatc ggctgagcgg cgtcccgccc ttgcggcgca
     5401 tgggctctcc cgctagggcg gcggcagccg gaaaggagcc gccgccccat gatcaagtcg
     5461 ctcaagcgcc tgttcgtcga ccacccgaag agcgtcgatg aaaattacat cgagcatttc
     5521 ggcgtcgcat cgaaattcgg ggtgacgatg atctatggcg gcctctgcgc gctcgtccat
     5581 gcgatcgtcc ccggctggtg catcaccacg ggcagcgaca cgatccagcg gctcaaccat
     5641 atcatggtcg aaaagcggcg cgccaagggg caggccgtga cgcagatgag cacgatcgac
     5701 tgggtgatct gagcggcgtg cgcccttctg gcacactcaa atctccgttc gcatcgagcg
     5761 aagtcgagat gcccctcggc tcggcgctac ctcgatgggt gtctcgactt cgctcgacac
     5821 gaacgggaat agaggatcgc gtcaattgaa cgagctcgcc cccgattatc tcgaacgctc
     5881 gggccgcccg cggctcgcct ttcgtcacac gccgggcgtc ggcccgacga tcgtcttcct
     5941 gcccggatac atgtccgaca tggcaggcgg caaggccacc gcgctgttcg actgggcggt
     6001 tgccgaaggc cacgcctgcc tgctgctcga ttatgccgga tgcgggctat cggacggatt
     6061 gttcgccgac cagacgctgc tcgattggcg cggcgacata ctcgacctga tcgacgcgaa
     6121 ggccgaagga ccggtcgtca tcgtcggatc gtcgatgggc ggctggctga tgctgctcac
     6181 cgcattggcg ctggtgcagc gcgacggacc cggccgcgtc gcggggctcg tcgggatcgc
     6241 ggcggcgccc gatttcaccg actggggctt cacgccggaa gaaaaggcga tcatccaggc
     6301 cgaaggcgcg ctggtcgagg aaacgcccta cagcgaccag ccctatatga cgacgcgcgg
     6361 cttttttcag tcgggcgagg ccaaccgcct gctcgacagc gacatccccc tcgcctgtcc
     6421 cgtgcgcctg ctgcacgggg aggaggacgg cgacgtcccg ccgagcatca gcctgcgcct
     6481 gtcggccgcg ctttcgagcg aggacgtgca ggtgacgttg gtcaagggcg gcgaccaccg
     6541 cctgtcgcgc gataccgaca tcgcgctgct gatcgatacg gtcgcgcggc tcgccacgca
     6601 aatctagaag aggacttcca tggcccgcag cgacttccgg ttccacgtca ccaagcgcgt
     6661 ccgctatgcc gagatcgacg cgcagcgcgt cgtgttcaac agccgctacc tcgaatattt
     6721 cgatatcggg atcaccgaat attggcgcgc cgcgggcgtc tatgaccgct ggcccgatca
     6781 cagcagcccc gaattccacg tcgcgcgggc cgaagtcgat tacaaggttc cgatcctgct
     6841 cgacgaagag atcgacattt gcgtgcgcgc gtcgcgcgtc ggcaaaagct cgatgacctt
     6901 cctgttcgag ctgcacggcg cgggcaagga cgacctgcgc gccagcggac agctgatcaa
     6961 cgtccatgtc gccgaggcgc agggcgcgcc ggcgccggtg ccggacgaat tcctattcct
     7021 gttcgaagcg tttgaagcgc ggccccttcg cgcctaaata gggcgccatg accaaatatc
     7081 ttcacacgat gatccgcgtg tcggatcccg acgccaccgt caccttcttc aagctgatcg
     7141 ggctcgagga aacgcgccgc ttcgacaacg aagccggccg cttcacgctg atcttcctcg
     7201 ccgcgcccgg acagggcgac gtcgccgagg tcgagctcac ttataactgg ccgcccgccg
     7261 acggcagtcc cgccgaggaa tatacgggcg gccgcaattt cggtcatctc gcttatcggg
     7321 tcgacgacat ctatgcgacg tgccagcggt tgatggacgc cggggtgacg atcaaccgcc
     7381 cgccgcgcga cgggcatatg gcgttcgtgc gctcgcccga cggcatttcg gtcgagctgc
     7441 tgcaggaagg cacgctcgat cccgccgaac cctgggcctc gatgcccaac accggcgcct
     7501 ggtaggagcc ctcgcgatgg cgggcctgtt gatcaacatc gacgtgcccg acctcgccgc
     7561 tggcgagcgt ttctacaccg cggcgcttgg cctgtccgcc gcccgccggt tgggcgacga
     7621 catcgtcgaa ctgacgggat gcgaaatccc gatctatctg atcgccaagc ccgcggggtc
     7681 gatgatcggc cccgacggcg gcgactttcg ccgctacaac cggcactgga cgccggtgca
     7741 ccccgatttc accgtcgacg atctgggcga tgcgatcgcg cgcgcccttg acgccggcgc
     7801 ggtgcgggag ggcgagacat tggacctgcc ctatggcaga caggcgatgt tcgccgatcc
     7861 gttcggcaac ggattctgcc tgatcgagtt caacgagcgg ggctatgacg cccttctgac
     7921 ctgagcagga gaaccaaggc ccgctggcgc ccgctccgtc ttgaaagagc gagacgctgg
     7981 aaaggagata caatggctgc gctggacatc gtccgaattc ccgtcctcag cgacaattat
     8041 gtctggctgg tccatgatcc cgccagcgag gcgacgatgg tcgtcgatcc cgcggtcgcc
     8101 gagccggtgc tggaagcggc cgcagcgcgc ggctggacga tcaccgacat ctggaacacc
     8161 cactggcacc ctgatcacac cggcggcaat gccgcgatca aggaagcgac cggctgcacg
     8221 atcaccggcc ccgcggcgga atttgcgcgc atcccgacgc tcgacgtgca tgttaagggc
     8281 ggcgatcagg tgcggctcgg caatcacgtc gccaacgttt gggacgtgcc cgcgcatacc
     8341 gcggggcata tcgcctatca tttcgccgaa gacgccgcga tcttcgtcgg cgacaccatg
     8401 ttcgcgatgg gctgcggccg gctgttcgag ggcaccgccg agcagatgtt cgacaatatg
     8461 cgcaagctcg acgcgctcga cgatgcgacg cgcgtctact gcgcgcacga atatacgctg
     8521 tcgaacgcac gcttcgcggt gacggtcgat cccgataacg aagctcttgc cgcgcggctc
     8581 gccgcggtcg aagccgcccg cgcggagggc gaagcgacag taccgaccag catcggcgaa
     8641 gagcgggcaa ccaacccctt tttccgcgcg ccagacgcgg ccgaactcgc gcgcatccgc
     8701 gcgctgaagg atgccgcctg atgccgccgc ttcagcaccc attcatctgc aacggccata
     8761 ttggccgatc tcaaggagca atttc
//
LOCUS       DATMSU010000547         5295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_56778, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000547 DATMSU010000000
VERSION     DATMSU010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56778"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VN034_14645"
     CDS             <1..183
                     /locus_tag="VN034_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWV61899.1"
                     /translation="MKDIGVVNAPSGAPTLALTGGAAERLAQMTPPGYSAHIHLTLTD
                     DHPWAQAFVIIEAIKD"
     gene            191..1036
                     /gene="lepB"
                     /locus_tag="VN034_14650"
     CDS             191..1036
                     /gene="lepB"
                     /locus_tag="VN034_14650"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWV61900.1"
                     /translation="MAKRGSKTKAKDDGWGKLIRDVVVILLLVLGIHSCVAKPFYIPS
                     DSMMPVLRNGDRLIVSKYPYGWSYSSVSFHLAPKVEGRIFGKLPQRGDIVVLEHPVTR
                     IDYIKRVIGLPGDTIALRGGELSINGKPVKREVQPMLSIPVDPNLPGPDSSLYRFVNR
                     DAGGKPVLELPIVRETLPGGATFDTIDMGPGYATDDYGPITVPANHVFLMGDNRDGSA
                     DSRVDTSQKGLGGPVPFDAIAGRAEIISFSTDGTAEWYNPLSWFIALRSGRAGTDLRP
                     EREGN"
     gene            1056..2180
                     /locus_tag="VN034_14655"
     CDS             1056..2180
                     /locus_tag="VN034_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWV61901.1"
                     /translation="MAATRKPAATRTAASAAAKAEAHREAPGPTEIRSHLVRTELLKA
                     GVWLGLALLIGVCIVLIQPILLIFAAIVMASMLDGGTRLLGRVLPIARGWRLLIVCLS
                     LLAFLGWTILFAGSQIADQAATLQTVVMAQIERIAAWASDHGMGNFQLDTKTITDNIA
                     GTVGRVTAAVGTVLGGLTSLIMILVLGIFIAIEPRLYERGVAWMLPMKARENFYITTA
                     RMGFTLRRLMAGRLLGMLVEGIGTWLACLAVGIPMAALMGLLTGLLAFIPNIGAIVSG
                     VLLVLVGFSAGMDTGLWAIAIYFVVQTVDGYLIVPMVAKKTVDLAPALVLGAQILFGA
                     LLGLMGLFLADPIVAMIKVALEREAERNADTAKTKTAAGT"
     gene            complement(2215..2742)
                     /locus_tag="VN034_14660"
     CDS             complement(2215..2742)
                     /locus_tag="VN034_14660"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWV61902.1"
                     /translation="MSVTTVPLRPVSKGGLWLMWFGLAALLVVGAAFAWHMTPRIGFE
                     VVKEGTGASPTKADVVLVKYEGKLDDGTVFDSNEQAPMQVAQVVPGFSEALTRMKKGG
                     EYKITIPPQLGYGDRAVGPIPAGSTLHFDVTLLDFRSEAEVRAMQQQMQQMQQQQMMQ
                     QQGGPAGPPPGAPQP"
     gene            complement(3068..4660)
                     /locus_tag="VN034_14665"
     CDS             complement(3068..4660)
                     /locus_tag="VN034_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HWV61903.1"
                     /translation="MASNALPRRSAAAALPDDDSIPLHERVRYRGLLTVAVMGASIMQ
                     ILDTTIANVAIPHMQSALGATSETVTWVLTSYILASAIAMPITGWLADRIGRRELFLG
                     AVAGFIVASMACGAAQTLDQMVAFRFMQGIFAAFIGPLSQSVMLDINPPERHARAMSI
                     WGMGIMIGPILGPVLGGWLTESVNWRWVFYVNLPVGLLTLAMMWALLPATRRSDRKFD
                     LFGFSMLALGLAALQLMLDRGAHEDWFNSVEIWIECGVAIACLWMFFVHLITARGATL
                     FNRKMLADRNLVTAMGFMVVIGVVMFASMALLPPMLQNLFGWPVIDTGIVLAVRGVGI
                     LMSMWVAGQLLGRIDARWLVGTGLLVAAYSLWQMSHWSLAMGMEPVIVSGLVQGIGMG
                     LIFIPLNTMAFATIAPQHRTDGSSLLNLLRSLGASVGISVVTTLLGVNTQTSHEDLAA
                     HVTNSSVGLLDPSTSDRFGIAGDAAMAMVNAEINRQAAMVAYIDDFWLMMWVTLASVP
                     LVVLLRPPKPGGPRASAADMGH"
     gene            complement(4850..>5295)
                     /locus_tag="VN034_14670"
     CDS             complement(4850..>5295)
                     /locus_tag="VN034_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWV61904.1"
                     /translation="SQAQVNLDRTTVRAPGAGRIAQSDRLQVGQMMVAGLPAVTLVDT
                     AHPWVEANFKETDLANMRVGQRAEISFDAYPGLTVRGHVLTIGAGTGSEFSVLPAQNA
                     TGNWVKVTQRVPVRIAFDETPSRDMIAGLSADVRVFTSGGAVSGK"
BASE COUNT          924 a         1819 c         1679 g          873 t
ORIGIN      
        1 atgaaggaca tcggcgtcgt caacgcaccc tcgggcgccc cgacgctcgc gctcaccggc
       61 ggcgccgccg agcgcctcgc gcagatgacc ccgccgggct acagcgcgca tatccatctg
      121 acgctgaccg acgaccatcc ctgggcgcag gccttcgtca tcatcgaagc aatcaaggac
      181 tgacattttc atggcgaagc gtggttcgaa aaccaaggcg aaggacgacg gctggggcaa
      241 gctgatccgc gatgtcgtgg tgatattgct gctcgtgctc ggcatccaca gctgcgtcgc
      301 caaacccttc tatatcccgt ctgattcgat gatgccggtg ctgcgcaacg gcgaccggct
      361 gatcgtcagc aaatatccct atggctggtc ctattcgtcg gtcagcttcc acctcgcgcc
      421 caaggtcgag gggcggatct tcggcaagct gccgcagcgc ggcgatatcg tcgtgctcga
      481 gcatccggtg acgcgtatcg actatatcaa gcgcgtgatc ggcctgcccg gcgacacgat
      541 cgcactccgg ggcggcgaac tcagcatcaa cggcaagccg gtgaagcgcg aggtgcagcc
      601 gatgctgtcg atcccggtcg accccaacct gcccggtccc gacagcagcc tgtaccgttt
      661 cgtgaaccgc gatgcgggcg gcaagcccgt gctcgaactg ccgatcgtgc gcgaaacgct
      721 gcccgggggc gccactttcg acacgatcga catgggcccc ggctatgcga ccgacgatta
      781 cggcccgatc accgtccccg cgaaccatgt tttcctgatg ggcgacaacc gcgacggcag
      841 cgccgacagc cgcgtcgaca ccagccagaa agggctcggc ggccccgtcc ccttcgacgc
      901 gatcgccggg cgcgcggaga tcatcagttt ttcgaccgat ggaaccgccg agtggtataa
      961 cccattgagc tggtttatcg ccctccgatc gggacgcgcc ggaaccgacc tgcggcccga
     1021 gcgtgagggc aattaggcaa aacggggaac acggcatggc ggcgacacga aaacctgcag
     1081 caacacgcac cgccgcatcc gccgccgcca aggccgaggc acaccgcgaa gcccccggcc
     1141 ccaccgaaat ccgcagccat cttgtccgca ccgagctttt gaaggcgggc gtgtggctcg
     1201 gcctcgcatt gctcatcggc gtgtgcatcg tgctgatcca gccgatcctg ctgatcttcg
     1261 cggccatcgt catggcgtcg atgctcgacg gcggcacccg cctgctcggc cgcgtgctgc
     1321 cgatcgcgcg cggctggcgc ttgctgatcg tctgcctctc gctcctcgcc ttcctcggct
     1381 ggacgatcct gttcgcggga tcgcagatcg ccgatcaggc cgcgacgctg cagaccgtcg
     1441 tgatggcgca gatcgagcgc atcgccgcgt gggcgagcga tcatggcatg ggcaatttcc
     1501 agctcgatac caagacgatc accgacaata tcgccggcac cgtcggccgc gtcaccgccg
     1561 cggtcggcac cgtgctcggc gggctgacca gcctgatcat gatcctcgtg ctcggcatct
     1621 tcatcgcgat cgaaccgcgg ctttacgaac gcggcgtcgc gtggatgctg ccgatgaaag
     1681 cccgagagaa tttctacatc accaccgcgc gcatgggctt cacgctgcgc cgcctgatgg
     1741 cggggcgcct gctcggcatg ctcgtcgagg gcatcggcac ctggctcgcc tgcctcgccg
     1801 tcggcattcc gatggcggcg ctgatggggc tcctcaccgg cctgctcgcc ttcatcccca
     1861 atatcggcgc gatcgtctcg ggcgtgctgc tcgtccttgt cggcttttcg gcggggatgg
     1921 acaccggcct ctgggcgatc gccatctatt tcgtcgtcca gaccgtcgac ggctacctga
     1981 tcgtcccgat ggtcgcgaaa aagaccgtcg acctcgcccc cgcgctcgtc cttggcgcgc
     2041 agatcctctt cggcgcgctg ctcggcctga tgggcctgtt cctcgccgac ccgatcgtcg
     2101 cgatgatcaa ggtcgcgctg gagcgcgagg ccgaacgcaa cgcggacacc gccaaaacga
     2161 aaacggcggc gggcacctag cccgccgccg ttttggctac atttgtttgc ccggttacgg
     2221 ctgcggcgcg cccggcggcg ggccggccgg accaccctgc tgctgcatca tctgttgctg
     2281 ctgcatttgc tgcatctgct gctgcatcgc gcgaacctcg gcttccgagc ggaagtcgag
     2341 cagggtgacg tcgaagtgca gtgtgctgcc cgccgggatc ggccccacgg cacggtcgcc
     2401 atagccgagc tgcggcggaa tggtgatctt gtattcgccg cccttcttca tgcgcgtcag
     2461 cgcttcggaa aagccgggaa cgacctgcgc gacctgcatc ggcgcctgtt cgttggaatc
     2521 gaacaccgtg ccgtcgtcga gcttgccctc atatttgacc agcacgacat cggccttggt
     2581 cgggcttgcg cccgtacctt ccttgacgac ctcaaacccg atccgcgggg tcatatgcca
     2641 ggcaaaagcc gcgccgacga caagcagcgc cgcgaggccg aaccacatca gccacagccc
     2701 gcccttgctc accgggcgca atggaaccgt cgttacgctc atcttggatg atccttccgc
     2761 tgccccgacc ggggcgaaaa tgctggcgag gcctataggc ggagcggtgc ggaacggtcc
     2821 agaggcaata tgcgaggtcg ccatatgagg aatgtcggct tgggggtggg tagctgttct
     2881 tcaatcctcg tcatgctgaa cttgtttcag catccatggc ctgcactcca attcagcgct
     2941 gcgccggagg aaacggcaga ccatggaccc tgaaacaagt tcagggtgac gagaaatgag
     3001 atgtccgcta tcggtcgcga gcggtcataa atggccgggg cgcccccgac gcccgccgta
     3061 atcgcggcta atgccccatg tccgccgccg acgctctcgg cccgcccggc ttcggcgggc
     3121 gcagcagcac gaccagcggc accgacgcca gcgtcaccca catcatcagc cagaaatcgt
     3181 cgatataggc gaccatcgcc gcctgccggt tgatctcggc attgaccatc gccatcgccg
     3241 cgtcgcccgc gatgccgaaa cggtcggagg tcgacgggtc gagcaggccg accgagctgt
     3301 tggtgacatg cgccgccaga tcctcgtggc tcgtctgcgt gttcacgccg agcagggtgg
     3361 tgacgaccga aatgccgacc gacgcgccca ggctgcggag caggttgagc aggctcgacc
     3421 cgtcggtgcg atgctgcggc gcgatcgtcg cgaacgccat ggtgttgagc ggaatgaaga
     3481 tcaggcccat gccgatcccc tgcaccagcc cgctgacgat caccggctcc attcccatcg
     3541 cgagcgacca gtggctcatc tgccatagcg aataggcggc gacgagcagg ccggtgccga
     3601 cgagccagcg cgcgtcgatc cggccgagca attgccccgc gacccacatg ctcatcaaaa
     3661 tgccgacccc gcgcaccgcg aggacgatcc cggtgtcgat caccggccag ccgaacagat
     3721 tttgcagcat cggcggcagc agcgccatcg atgcgaacat cacgacgccg atcacgacca
     3781 tgaaacccat cgcggtgacg aggttgcggt cggcgagcat cttgcggttg aacagcgtag
     3841 cgccgcgcgc ggtgatcaga tggacgaaga acatccacag gcacgcgatc gcgactccgc
     3901 actcgatcca gatttcgacg ctgttgaacc agtcctcgtg cgcgccgcgg tcgagcatca
     3961 gctggagcgc ggcgaggccg agcgcgagca tcgagaaacc gaacaggtcg aacttgcggt
     4021 cggagcggcg cgtcgcgggc agcagcgccc acatcatcgc gagcgtgagc aatccgacgg
     4081 gcaggttgac gtaaaagacc cagcgccagt tgacgctttc ggtcagccag ccgccgagca
     4141 cggggcccag aatcggcccg atcatgatcc ccatgcccca gatcgacatc gcgcgcgcgt
     4201 ggcgctcggg cgggttgatg tcgagcatca ccgactgcga cagcggcccg atgaaggccg
     4261 cgaaaatccc ctgcatgaag cggaaggcga ccatctggtc gagcgtctgc gcggcgccgc
     4321 acgccatcga cgcgacgatg aagcccgcga ccgcgccgag gaacagctcg cgccgcccga
     4381 tgcggtcggc gagccagccg gtgatcggca tcgcgatcgc cgaggcgagg atatagctcg
     4441 tcagcaccca ggtcaccgtc tcgctcgtcg cgccgagcgc cgactgcatg tgcgggatgg
     4501 cgacgttggc gatcgtggtg tcgaggatct gcatgatcga cgcgcccatg acggcgacgg
     4561 tcaagaggcc gcgatagcgg acgcgctcgt ggagcgggat cgaatcgtcg tcgggcaagg
     4621 ccgcggcggc ggaccggcgg ggaagggcgt tgctggccat cagcttatct ttgccctctc
     4681 cccttcaggg gagaggatat gcaggcttgc gagcttgctc gctagccgga gttggagagg
     4741 ggaaccgacg ccgcgatccc ctctccaagc tgcgctagct cctgcggagc aagcttcgct
     4801 atcctctccc ctgaagggga gagggccagg agaatgccgc gacgtcaccc tatttccccg
     4861 acaccgcgcc gccgctggta aacacccgca catccgccga cagccccgcg atcatgtcgc
     4921 gcgacggcgt ttcgtcgaag gcgatccgca ccggcacgcg ctgcgtcacc ttgacccagt
     4981 tgccggtggc gttctgcgcg ggcagcaccg aaaattcgct gcccgtgccc gcgccgatcg
     5041 tcagcacatg gccgcgcacc gtcagtcccg gataggcgtc gaagctgatt tcggcgcgct
     5101 ggccgacgcg catgttcgca aggtcggttt ccttgaaatt ggcctcgacc cacggatgcg
     5161 cggtgtcgac cagcgtcacc gcgggcagtc ccgcgaccat catctggccg acctgcaacc
     5221 ggtccgactg cgcgatgcgg cccgcgcccg gcgcgcgcac cgtcgtccgg tcgaggttca
     5281 cctgcgcctg cgacc
//
LOCUS       DATMSU010000548         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_27985, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000548 DATMSU010000000
VERSION     DATMSU010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27985"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..1720)
                     /locus_tag="VN034_14675"
     CDS             complement(239..1720)
                     /locus_tag="VN034_14675"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HWV61905.1"
                     /translation="MTSNSSQDTTDPTALVDGIIAELQDRFRISVSNLKSAIAAYVRD
                     RTLPPADAAAKGLFDYPAIRLTTTGEQREGQKGHNLAFGRFERAGTFVTTVTRPDLFA
                     DYLREQLMLLARHYDIALEVTRTGQQIPFPYVLDASDGSDMGGVTPLELARHFPTTEL
                     ADIGDELADGLFGQDPAADQPLALFDALRTDFSLARLRHYTGTAPEHFQRYILFTNYH
                     RYVDEFVAWAGAQVGQGKYTALAGAAGLYVDQPGVNASALVADSAWRRHQMPAYHLIA
                     PDGGGITLVNIGVGPSNAKTICDHLAVLRPEAWLMIGHCGGLRPSQRIGDYVLAHAYL
                     RDDHILDAVLPVAIPIPPIAEVQVALASAAEAVSGTSGADLKKRMRTGTVVTTDDRNW
                     ELRYTASALRFSQSRAIGIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGELKLPGQA
                     NRFYEEAIAAHLQIGIRTCELLRDEGAKLHSRKLRAFNEPPFR"
     gene            complement(1811..>4360)
                     /locus_tag="VN034_14680"
     CDS             complement(1811..>4360)
                     /locus_tag="VN034_14680"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61906.1"
                     /translation="GNPPDGGGVGAGAAEIDLRYLGSRRVLVLVDGIRYVNGASASGV
                     PGSTDLNSIPESAIERVEVLQDGASAIYGSDAIAGVVNIITKRSQEGLRASAQVGGFG
                     QGDGFTKSAQVSWGNGRGPTQIVVGANYVQQDGVFAGDRAISRFPAPYSDTCLDGGCS
                     GFVPLGRFIVFGTPGARNDLTLRGPVIGRNPVYNPGNPTDPASDFKAFTDADRFNFGP
                     FNYLQIPVERYGAFINARQELSDSINFSVRGIWNQRKSKNRAAPLPFGFGPAAGLTPV
                     LDAITVDATNPFNPFGVTLDASNTDLVYRRFVEGGPRRFNQTVTTIYGVATLDGSFDV
                     GGGKWYWDVNAAYGRNKARQTMFGNINSDRLRRALGPVADCTGECVPFNIFGGVGSIT
                     QPMLDYVMFTQRDRSRQSTFSASANVTGSLFELPGGPLGVAVGVEYRKLKGSFDPDPI
                     VAAGFSSDIPALPTRGSYNVKEAYAELSAPVLSDIAFIDLLEFTGAVRWSDYSTSGST
                     TTLKGGVNWKPIPDLRLRASYAEGFRAPSIGELFGTQSRFDQSINDPCSTSTTTGQNW
                     NNNSTVRANCIAQGVPTAGYAQANPQISVLVGGNQDLAPESSRSWIFGGVYSPGFVPG
                     LSLEANYYDIKVKGATQAVNAEITLAQCVGQNDADACALVTRAAGGQLTFVEGLLQNI
                     AGIRTKGVDVNLSYRTQETGAGRFGFTWNNSFLTQYDVIVPTADGRLVVSREGTEQGS
                     PSQAFPKWKSVGILDWDGASVGATLTGRYVSAIREPLADDNKLKARFYTDFQLRWFPA
                     DDPEAFGFALGVNNLFNVKAPGCISCDLNNFDPTAYDLPGRYYYVRASFKM"
BASE COUNT          724 a         1310 c         1578 g          748 t
ORIGIN      
        1 cggtcgcgcg gcccatgccg ctcgccgcgc cggtgacgat cgcgacgcgg ccggcgaccg
       61 agcgggagag gttggcgaag ttctgcatgg tcggattcac tcccttctcg tcatgctgaa
      121 ctcgtttcag catccatggc cagccctcgc ctcagacgcg gcgtgggagg taatgtcggg
      181 ccatggaccc tgaaacaagt tcagggtgac gcagaattgc agggcgtatc gtttcggctc
      241 accgaaatgg cggctcgttg aacgcgcgca atttccggct gtgaagcttg gcgccttcgt
      301 cgcggagcag ttcgcaggtg cggatgccga tctggaggtg cgcggcgatc gcttcctcat
      361 agaagcggtt cgcctgtccg ggcagcttga gctcgccgtg gagcggcttg tcggaaacgc
      421 agagcagggt gccatagggg acgcggaagc gatagccctg cgcggcgatc gtcgccgatt
      481 ccatgtcgat gccgatcgcg cgcgactgcg agaagcgcag cgcgctggcg gtgtagcgca
      541 gctcccagtt gcggtcgtcg gtggtgacga cggtgccggt gcgcatgcgt ttcttgaggt
      601 cggcgccgct ggtgcccgac accgcctcgg cggcgctggc gagcgcgacc tggacttcgg
      661 cgatcggcgg gatcgggatg gcgaccggca gcaccgcgtc gaggatatgg tcgtcgcgaa
      721 gataggcgtg cgcgagcaca taatcgccga tgcgctggct ggggcgcagc ccgccgcagt
      781 ggccgatcat cagccaggct tcggggcgca gcaccgcgag atggtcgcag atcgtcttgg
      841 cgttcgacgg gccgacgccg atgttgacga gcgtgatgcc gccgccatcg ggcgcgatga
      901 ggtgataggc gggcatctgg tgccgccgcc acgcgctgtc ggcgacgagg gcgctggcgt
      961 tgacgccggg ctggtcgaca tagaggcccg cagcacccgc gagcgcggta tatttgccct
     1021 ggccgacctg cgcgcccgcc caggcgacga attcgtcgac atagcgatga tagttggtga
     1081 agaggatgta gcgctgaaaa tgctcgggcg cggtgccggt gtaatggcga aggcgcgcga
     1141 ggctgaagtc ggtgcgtagc gcgtcgaata gcgcgagcgg ctggtcggcg gcgggatcct
     1201 gtccgaacag cccgtcggca agctcgtcgc cgatgtcggc gagctcggtc gtcgggaaat
     1261 ggcgcgcgag ttcgagcggg gtgacgccgc ccatgtccga cccgtcgctc gcgtcgagca
     1321 cataggggaa ggggatttgc tgcccggtgc gcgtgacttc gagcgcgatg tcatagtggc
     1381 gcgcgaggag catcagctgt tcgcgaagat aatcggcgaa aaggtcgggg cgcgtcacgg
     1441 tggtgacgaa ggtgcccgca cgctcgaagc ggccgaaggc gagattatgg cccttctgac
     1501 cctcgcgctg ctcgccggtg gtcgtgaggc ggatcgccgg atagtcgaac agacccttgg
     1561 cggcggcgtc ggctggcggc agcgtgcggt cgcgcacata ggccgcgatc gccgatttga
     1621 gattggagac cgagatgcgg aaacgatcct gcagttcggc aatgatgccg tcgacgagtg
     1681 cggtgggatc ggtggtgtct tgcgatgagt tcgatgtcat cgcgtcgctg ttgcatgaaa
     1741 atgtgacaaa agggaagggg ggcgaaagac agcccctccc cggcaaggga ggggctgcgc
     1801 gcgttcgacc ctacattttg aagctcgccc ggacgtaata atagcgcccg ggcagatcat
     1861 aggcggtggg atcgaaattg ttgaggtcgc agctgatgca ccccggcgcc ttgacgttga
     1921 acaggttgtt cacgccgagc gcgaagccga aggcttcggg atcgtccgcc gggaaccagc
     1981 gcagctgaaa gtcggtgtag aagcgcgcct tcaacttgtt gtcgtcggcg agcggttcgc
     2041 ggatcgccga gacatagcgg ccggtcagcg tcgcgccgac cgatgcgccg tcccagtcga
     2101 ggatgccgac cgacttccat ttcgggaagg cctggctggg gctgccctgc tcggtgcctt
     2161 cgcggctgac gacgagccgg ccgtcggcgg tgggcacgat cacatcatat tgggtgagga
     2221 acgagttgtt ccaagtgaag ccgaaccgtc ccgccccggt ttcctgcgtc cggtacgaga
     2281 ggttgacgtc gacccccttg gtgcggatgc cggcgatatt ctggagcagc ccttcgacga
     2341 aggtcagctg gccgcccgca gcgcgggtga cgagcgcgca ggcgtcggcg tcattctgtc
     2401 cgacgcattg cgccagcgtg atctcggcat tcaccgcctg ggtcgcgccc ttgaccttga
     2461 tgtcgtaata atttgcctcg agcgacaggc cggggacgaa gcccggactg tatacgccgc
     2521 cgaaaatcca gctccgcgag ctttcgggag cgaggtcctg attgccgccg acgagcaccg
     2581 agatttgcgg attggcctgc gcatagcccg ccgtcgggac gccctgcgcg atgcaattgg
     2641 cgcggacggt cgagttattg ttccagttct gccccgtggt ggtcgaggtc gagcaggggt
     2701 cgttgatcga ctggtcgaag cgcgactggg tgccgaacag ctcgccgatc gaaggcgcgc
     2761 ggaaaccctc ggcataggag gcgcggaggc gcaggtccgg gatcggcttc cagttgacgc
     2821 cgcctttcaa tgtcgttgtc gagcccgagg tcgaataatc agaccagcgc accgcgccgg
     2881 tgaattcgag caggtcgatg aaggcgatgt cggacaggac cggcgcgctg agctcggcat
     2941 aggcttcctt gacattatag ctgccgcgcg tcggcagcgc cggaatgtcc gagctgaagc
     3001 ccgcggcgac gatcgggtcg ggatcgaagc tgcccttgag tttgcgatac tcgaccccga
     3061 ccgcgacgcc gagcgggccg ccgggcagct cgaagaggct gccggtgacg ttggccgagg
     3121 cgctgaaggt cgactggcgg ctgcggtcgc gctgggtgaa catgacatag tcgagcatcg
     3181 gctgggtgat cgacccgacc ccgccgaaga tgttgaaggg cacgcattcg ccggtgcagt
     3241 cggcgaccgg gccgagcgcg cggcgcaggc ggtcggaatt gatgttgccg aacatcgtct
     3301 gcctggcctt gttgcggcca taggcggcat tgacgtccca ataccatttg ccgccgccga
     3361 cgtcgaagct gccgtcgagc gtcgcgacgc cgtatatcgt cgtcacggtc tggttgaagc
     3421 ggcgcggccc gccctcgacg aagcggcgat agacgagatc ggtattgctc gcgtcgagcg
     3481 tcaccccgaa cggattgaag gggttggtgg cgtcgacggt gatcgcgtcg agcaccgggg
     3541 tcaaccccgc ggcggggccg aagccgaagg gcagcggcgc ggcccggttt ttcgatttgc
     3601 gctggttcca gatcccgcgg accgagaaat tgatgctgtc gctgagctcc tggcgcgcgt
     3661 tgataaaggc gccatagcgt tcgaccggaa tctggagata gttgaacggg ccgaaattga
     3721 agcggtcggc gtcggtgaac gccttgaaat cgctcgccgg gtcggtcgga ttgccggggt
     3781 tgtacacggg gttgcggccg atcaccgggc cgcgcagcgt cagatcgttt cgggccccgg
     3841 gcgtgccgaa gacgataaag cgtcctagcg gcacaaatcc cgagcagccg ccgtcgaggc
     3901 aggtgtccga ataaggcgcc ggaaagcgcg aaatcgcgcg gtcgcccgcg aagacgccgt
     3961 cctgctggac ataattggca ccgacgacga tctgggtcgg gccgcggccg ttgccccagc
     4021 tgacctgcgc gctcttggta aagccgtcgc cctgcccgaa gccgccgacc tgcgcgctgg
     4081 cgcgcagccc ttcctggctg cgcttggtga tgatgttgac gacgcccgcg atcgcatccg
     4141 acccgtagat cgccgaagcg ccgtcctgca gcacctcgac ccgctcgatc gcgctttcgg
     4201 gaatcgagtt gaggtcggtc gagccgggca cgccgctcgc cgacgcgccg ttcacatagc
     4261 ggatgccgtc gacgagcacg agcacgcggc gcgagcccag ataacgcagg tcgatctcgg
     4321 ccgcgccggc gccgacgccg ccgccgtcgg gcgggttgcc
//
LOCUS       DATMSU010000549         8314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_179917,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000549 DATMSU010000000
VERSION     DATMSU010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179917"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VN034_14685"
     CDS             <1..182
                     /locus_tag="VN034_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61907.1"
                     /translation="IGEIVAWATGEPAVEAITAETSVANLPSERVLLRNGFGKVGERL
                     DEQDGPLNCWRRDVA"
     gene            complement(198..542)
                     /locus_tag="VN034_14690"
     CDS             complement(198..542)
                     /locus_tag="VN034_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61908.1"
                     /translation="MAKLPKPGQPVRGSRSGAPIMALFDLLGRRWAMGVLWTLSEGGA
                     MTFRELQDRCETISPSVLNQRLSELKEAGFVTRSDRGYVPTPLGERIYRHLVPLGTAA
                     RDWSDALDEGPD"
     gene            718..1413
                     /locus_tag="VN034_14695"
     CDS             718..1413
                     /locus_tag="VN034_14695"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWV61909.1"
                     /translation="MKPLLYSGTKNASSWAMRAWLALREAGIDFDEEVVDIRRPQRFA
                     NLERIGAFSPPAMVPVLVVDQMAIFDSVAIMEYANDVADGALLPADRAARARARSLIA
                     WQHSGLSGICARISFESAFYPLKRSLTDAERTECGRLFDCLEQALELSGGPYLFGTLS
                     LADLMLVPTVVRLTRHDLDLASWPRSGRWAAALLERASVAEWMREAEALPHIWFDDYL
                     VPGDAVRMVPAAA"
     gene            1426..1899
                     /locus_tag="VN034_14700"
     CDS             1426..1899
                     /locus_tag="VN034_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWV61910.1"
                     /translation="MATQHDFTSRIEWTGNRGEGTRSYRGYDRSWSVTTPGQPVIHCS
                     NDPRLGGNPALPNPEDMLLSALAACHMLWYLHLASEAGIVVQSYHDTPIGAAESTPDG
                     AGRFVRAILKPHIVVERDADLARADAIHHEIHKVCFIARSVNFPVSYEASYAAAE"
     gene            complement(1912..3606)
                     /locus_tag="VN034_14705"
     CDS             complement(1912..3606)
                     /locus_tag="VN034_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWV61911.1"
                     /translation="MLLLALLAPVVGTGGAFGAWILIKSIALATNLFWFGRISVEEVE
                     ISDAAVGLAVVAIPVIGALIIGLMARFGSEKIRGHGIPEAIETILFGESRLSLKVAIL
                     KPVSAAISIGSGGPFGAEGPIIMTGGAVGSLFAQCFHLSAAERKTLLVAGAAAGMTAI
                     FGTPMAAILLAVELLLFEWKPRSFVPVVISVLVAAAWRPELIGHGPMFPAVWPLPEAG
                     WTVATAAGIGILVGLQAALLSTCLYRIEDLFHRLPVHWMWWPAIGAVVVGVGGLIDAR
                     VLGAGYSNIQALIDGSIALRAVLVLLVVKAVVWLVALGSGTSGGVLAPLLILGGATGY
                     IVGLWLPGSAGFWAMVGMAGVISGAMRAPLTGILFAAELTGRFDALPAVIAGSGFAYA
                     ISVLTMRRSILTEKIARRGRHILQEYTVDPLDLRQAGQLMTPNPATLPAEMTIAAALR
                     FFTGEATHRSYPVVDAEGRLVGLASRSDALRWQVGAFDEAATLGETLSDASQTIAHPE
                     TPCGVIADLIVETGVARVPIVDRETGKVVGILSRQDLLKARRAQQNAETERTRFGG"
     gene            3750..4154
                     /locus_tag="VN034_14710"
     CDS             3750..4154
                     /locus_tag="VN034_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWV61912.1"
                     /translation="MTANSTDIDYAALAEFRFALRRFQVFSEARAAEAGLTPQQHQAL
                     LAIRAAEPDEATVGYVAQRLIVKPHSASELIDRLEAQSLVRREPTRSDRRRALLRLMP
                     QAIEILSDLSAAHREEIRRLQPLLVQHLTRLT"
     gene            4256..6433
                     /locus_tag="VN034_14715"
     CDS             4256..6433
                     /locus_tag="VN034_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HWV61913.1"
                     /translation="MFRSFRPVFKFALLAASALSVPAFAQEASPEQEEGEASGRKDDT
                     IVVYGSGIDRNTAATGLDLTPRETPQSITIITREQIDDQAASTVADVLEYTTGLSVKR
                     VDRGRNLLSARGFDITAFQIDGLPFATGNVGLEETSTAIYDRVEVIRGATGLLQGAGE
                     PSASINMVRKRADARELTGEVNFEAGSWNHYSGLIDIGAPLTADGSVRARLVAEVYSQ
                     DAFVDLESSNGFTLYGTIAADLGPATRLRAGASYQRDERDGVMWAQLPYWYSDGSRPD
                     WSRSKTTGADWNEWDTTEKAAFVSLEQDLGGSWQLRGDVSYFEQVEDSKLIWLWGNPD
                     RETGTGMEIWPYWYLSKPRQWSANLRVKGDYQLFGRRHELVFGAMYSHQKGGWTNRDP
                     DPASVAPVGDFHAWDGSYPEPVWGERYRMSGFGTTEQTAIYGVTRFQILDRLKLIAGG
                     RLSSWTRDEEEAAFTPEPYRIEHKNVFTPYAGLILDLGDFLSAYASYTSIFNPQTARD
                     RTGRYLDPLEGDNYEAGLKADLMDGRLRASAAVFRVEQSNFAVPDIDPETGDPYLIPG
                     TPDVASRPAKGVVSKGYELEMQGEPLPGWDISVGWSHFKAKDPDGVDVQAHQPRRVFR
                     MATKYEFGGALNGFSLGGSLRWESRPPQTAPNPVTEQDEPVGQKAYALVNLMGAYDLT
                     EAVSLQVNVNNVFDKAYYNTNSWFGGFIYGEPRNVRVTLRYGF"
     gene            6976..7446
                     /locus_tag="VN034_14720"
     CDS             6976..7446
                     /locus_tag="VN034_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61914.1"
                     /translation="MGRNGKAAEGSGNARPGRRQLDIFLESLAESSNVAASARAAEIS
                     ANAMYRERRRNPGFAARWHEALCEGFARLEAELLSEALIAPSGNVKDATLKSRAQKYR
                     LGLSLLAAHRAAVRGARLPAAGAMPKGGAKARLIAKLDAMRAQVAAENAEEGLL"
     gene            7443..>8314
                     /locus_tag="VN034_14725"
     CDS             7443..>8314
                     /locus_tag="VN034_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HWV61915.1"
                     /translation="MKDLAELLTMPPAEYAAWAHRLRDDKAAELWTDWSWWRRGDQCP
                     PAGDWHVWMLLAGRGFGKTRTGAEWIRGYAETHPGARIALVAASLHEGRQVMIEGESG
                     LLAIAPDDNRPDYESSLRRLSWANGAVATLYSAAEPESLRGPEHSAAWCDEIAKWPQG
                     EAAWDNLMLTMRIGDNPRVVATTTPRGVPLVRRLIDEKGVETTGGSTRSNRRNLSPQW
                     LATMDRLYGGTRLGRQELGGELLEDVEGALWTRALVERCRVEADATAKPVRVVIGVDP
                     PATANGDACGIVVAV"
BASE COUNT         1425 a         2636 c         2848 g         1405 t
ORIGIN      
        1 cgatcggcga gatcgtcgcc tgggcgacgg gcgagcccgc ggtcgaggcg atcaccgcgg
       61 agacgagcgt cgcgaatctg ccgtccgaac gcgtcctgct gcgcaacggc ttcggcaagg
      121 tcggcgagcg gctcgacgaa caggacgggc cgctgaactg ctggcgccgc gacgtcgcct
      181 gatcggggag ccgagcttca gtccggccct tcgtccagcg catcggacca atcgcgcgcc
      241 gcggtgccca gcggaacgag gtgccggtag atgcgctcgc cgagcggtgt cggaacatat
      301 ccgcggtccg atcgcgtcac gaatccggcc tccttcagtt cgctgaggcg ctggttcagc
      361 accgaggggg agatcgtttc gcatcggtcc tgaagttcgc ggaaggtcat cgctccgcct
      421 tcgctgagcg tccagagcac gcccatcgcc cagcggcgac cgagcagatc gaacagcgcc
      481 atgatcggcg cgcccgagcg cgagccgcga acgggctggc cgggtttggg cagtttcgcc
      541 atcgattcct cctgcgccgg gctcggtcct gtgcgagtgc ggacgatcgt tgctcctctg
      601 gtagcgcgct agctttttga tagctagcta gctgcggaag aagcgactcg ggaatgtact
      661 tgctatcgat tcgatagcac gctatgaaat tcgtagcaac gctatgggat attcgccatg
      721 aaaccgctcc tctattcggg aacgaagaac gcctcgagct gggcgatgcg cgcgtggctc
      781 gcgctgcgcg aggcggggat cgactttgac gaggaggtcg tcgacatccg gcggccgcag
      841 cggttcgcga atctggagcg gatcggggcc ttctcgcccc ccgccatggt tcctgtgctg
      901 gtcgtcgatc aaatggcgat cttcgattcg gtggcgatca tggaatatgc caacgatgtc
      961 gcggacgggg cgttgctccc cgccgaccgc gcggcgcgcg cccgcgcacg atcgcttatc
     1021 gcctggcaac attcggggct gtcgggcatt tgcgcgcgaa tctcgttcga aagcgccttc
     1081 tatccgctca agcgatcgct gaccgatgcc gagcgcaccg aatgcggacg ccttttcgac
     1141 tgcctcgagc aggcgctgga gctgagcggc gggccgtatc tgttcgggac gctgagcctt
     1201 gcggacctga tgctcgtgcc gacggtggtg cgcctgacgc ggcacgacct cgacctcgca
     1261 agctggcccc ggtcgggacg ttgggcggcg gcgctgctcg aacgcgcgtc ggttgccgaa
     1321 tggatgcgcg aggcggaggc gcttccccat atctggttcg acgactatct ggtgcccggc
     1381 gacgcggtcc ggatggttcc ggcagcggcc tgaggaggac ttatcgtggc gacccagcat
     1441 gattttacga gccgtatcga gtggaccggc aatcgcggcg agggaacgcg tagctaccgc
     1501 ggctatgacc gcagctggag cgtgaccacg ccgggccagc cggtgatcca ttgttcgaac
     1561 gatccgcggc tgggcggaaa cccggcgctg cccaatcccg aggatatgct gctgtcggcg
     1621 ctcgccgcct gccacatgct ctggtatctc catctggcca gcgaggccgg gatcgtcgtg
     1681 caatcctatc atgacacgcc gatcggggcg gccgagagca cgccggacgg cgcgggccgc
     1741 ttcgttcgcg cgattctcaa accgcatatc gtcgtcgagc gtgacgccga tctggcgcgc
     1801 gcggatgcga tccaccatga aattcacaag gtgtgcttta tcgcgcgctc ggtgaacttc
     1861 cccgtgtctt acgaggcgag ttatgcggcc gccgaatgac ggggagcgat gctatccccc
     1921 gaatcgcgtc cgttccgtct ccgcattctg ttgcgcccgc cgcgccttga gcaggtcttg
     1981 ccgcgataga ataccgacga ccttgcccgt ttcgcggtcg acgattggga cgcgcgcgac
     2041 ccccgtctcg acgatgaggt cggcgatgac gccgcagggg gtttcgggat gtgcgatggt
     2101 ttgcgaggcg tccgagagcg tttcgccgag cgtcgccgct tcgtcgaacg cgccgacctg
     2161 ccatctgagt gcgtccgacc tcgacgcgag cccgaccagc cggccttcgg catcgacgac
     2221 cggatagctg cgatgcgtcg cctccccggt gaaaaagcgc agcgccgcgg cgatcgtcat
     2281 ctcggcggga agggtggcgg gattgggcgt catcagctgg ccggcctgcc gcaaatcgag
     2341 cgggtcgacg gtatattcct gcaggatatg ccgcccccgg cgggcgattt tttcggtcag
     2401 gatcgaccgc cgcatggtca gcacgctgat cgcgtaggcg aagcccgatc ccgcgatcac
     2461 cgcgggaagc gcgtcgaagc gcccggtcag ttcggctgcg aagaggatgc cggtgagcgg
     2521 cgcccgcatc gctccgctga tcacgcccgc catgccgacc atcgcccaga aacccgccga
     2581 gcccggaagc cacaggccga cgatatagcc ggtcgctccg ccgagaatga ggagcggagc
     2641 gagcacgccg cccgatgtgc ccgaaccgag cgcgacgagc cagacgacgg ccttcacgac
     2701 caggaggacg agcaccgcgc ggagcgcgat gctgccgtcg atcagcgcct gaatattcga
     2761 atagcccgcg cctaagacac gtgcgtcgat caatcccccg acgccgacga cgacggcgcc
     2821 gatcgccggc caccacatcc agtggacggg aaggcggtgg aacaggtcct cgatccgata
     2881 gaggcatgtc gagaggagcg cggcttgcaa gcctacgagg atgccgatcc cggccgcggt
     2941 ggccacggtc cagccggctt cgggtagcgg ccagactgcc ggaaacatgg ggccatgacc
     3001 gatgagttcg ggccgccatg ccgcggcgac caatacgctg atgacgaccg gcacgaagct
     3061 ccgcggcttc cattcgaaca gcagaagctc gaccgcgagc aggatcgccg ccatcggcgt
     3121 gccgaatatc gccgtcatgc ccgccgcggc gcccgccacc agcaacgtct tgcgttccgc
     3181 cgcgctgagg tgaaaacatt gcgcgaagag cgagccgacg gcgccgccgg tcatgatgat
     3241 cggtccctcg gcgccgaacg gcccgccgct tccgatcgat atggcggccg ataccggctt
     3301 gaggatcgcg accttcagcg aaaggcggct ttcgccgaac aggatcgtct cgatcgcctc
     3361 gggaatgccg tggccgcgta ttttttccga tccgaagcgt gccatcaacc cgatgatcaa
     3421 ggcgccgatc acgggaatcg ccacgacggc gagaccgacc gcggcatcgg agatttcgac
     3481 ttcctccacc gaaatccggc cgaaccagaa gaggttggtc gcgagcgcga tcgacttgat
     3541 caatatccac gcgccgaacg cgccgcccgt cccgacgacc ggcgccagca gggcgagaag
     3601 cagcattcgg ccgtcgacgc tgtggtcggc aagttgctgg ctgttgtccg gtcgtgcaga
     3661 atgaaggcgc accgcgtggc tctttcgatt gttcaggggg gcgcggggcc attatatcgt
     3721 aatacgacat aattcaacct gaggctttga tgaccgcgaa ttcgaccgat atcgactatg
     3781 ccgcgctcgc cgagttccgc ttcgcgctgc ggcgctttca ggtcttcagc gaagcgcggg
     3841 cggccgaagc cgggctgacg ccgcaacagc atcaggcgct gctggcgatc cgcgccgccg
     3901 agccggacga ggcgaccgtc ggatatgtgg cgcagcgcct gatcgtgaag ccgcactcgg
     3961 cgtccgagct gatcgaccgg ctcgaagcgc agagtcttgt ccggcgcgag ccaacgcgat
     4021 cggatcgacg gcgggcgctg ctgaggttga tgccgcaggc catcgagatt ttgtcggacc
     4081 tgtcggcggc gcaccgcgaa gagattcgcc gccttcagcc gctgctcgtc cagcatttga
     4141 cgcggttgac ataggcgccg gcggcatcgc ccctccttcc ccgcctgact ctattgcgtc
     4201 gcagttgcaa ctagtctagg ggcccccggt cgttacaggg atagtcgggg gcattatgtt
     4261 tcgttcgttt cgtccagtct tcaaattcgc gctgctcgcc gccagcgcgc tttcggttcc
     4321 ggcgttcgcg caggaagcgt ccccggagca ggaagagggc gaggcgagcg gccgcaagga
     4381 cgacacgatc gtcgtttatg gcagcggcat cgaccgcaac accgccgcca cgggactcga
     4441 tctgacgccg cgcgagacgc cgcagtcgat cacgatcatc acgcgcgagc agatcgacga
     4501 ccaggcggcg tcgacggtcg ccgacgtgct cgaatatacc accgggcttt cggtgaagcg
     4561 cgtcgatcgc ggccgcaacc tgctgtcggc gcgcggtttc gacatcaccg ccttccagat
     4621 cgacggtctg ccctttgcga cgggcaatgt cgggctcgag gaaacgagca cggcgatcta
     4681 tgaccgggtc gaggtcatcc gcggtgcgac cggcctgttg caaggggcgg gcgagccttc
     4741 ggcgtcgatc aacatggtcc gcaagcgcgc cgacgcccgc gagttgaccg gcgaagtgaa
     4801 tttcgaagcg ggttcgtgga atcattattc ggggctgatc gacatcgggg cgccgctgac
     4861 cgccgatggc tcggtgcgcg cgcgtcttgt cgccgaggtc tattcgcagg atgcctttgt
     4921 cgatctcgaa agcagcaacg gcttcaccct ctatggcacg attgccgccg atctcggccc
     4981 tgccacgcgg ctccgcgccg gggcgagcta tcaacgcgac gagcgcgacg gggtgatgtg
     5041 ggcgcagctg ccctattggt attccgacgg ctcgcgcccc gattggtcgc ggtcgaaaac
     5101 caccggcgcc gactggaacg aatgggacac caccgaaaag gcggcgttcg tgtcgctcga
     5161 gcaggatctg gggggcagct ggcaattgcg cggcgacgtt tcctatttcg agcaggtcga
     5221 ggattcgaag ctgatctggc tgtggggcaa ccccgaccgc gaaaccggga cggggatgga
     5281 aatatggccc tattggtatc tttccaagcc caggcaatgg agcgccaacc tgcgggtcaa
     5341 gggcgattac cagctcttcg gccgccggca cgaactggtg ttcggcgcga tgtacagcca
     5401 ccagaagggc ggctggacca atcgcgatcc cgaccccgcg agcgtcgcgc cggtcggcga
     5461 tttccacgca tgggacggca gctatcccga gcccgtgtgg ggggaacgtt atcgcatgag
     5521 cgggttcggc acgaccgaac agactgccat ctatggcgta acgcgcttcc agattctcga
     5581 ccggctcaag ctgatcgccg gcgggcggct gagttcgtgg acgcgcgacg aggaggaggc
     5641 ggcctttacg cctgaaccct atcggatcga gcacaagaat gtcttcacgc cctatgcggg
     5701 gctgatcctc gacctgggtg acttcctctc cgcctatgcg agctatacga gcatcttcaa
     5761 tccgcagacc gcgcgcgacc gcaccggccg ctatctcgac ccgctcgaag gcgacaatta
     5821 cgaagcgggg ctgaaggccg acctgatgga cggccgtctg cgcgcgtcgg cggcggtctt
     5881 ccgcgtcgag cagagcaatt tcgccgttcc cgacatcgac cccgaaacgg gcgatcccta
     5941 tctgattccc ggtacccccg acgtcgcctc acgccccgcc aagggcgtgg tgtcgaaggg
     6001 ctatgaactc gaaatgcagg gcgagccgct gccgggctgg gacatcagcg tcgggtggag
     6061 ccatttcaag gcgaaggatc ccgacggcgt cgatgtgcag gcgcatcagc cgcggcgcgt
     6121 gtttcgcatg gcgacgaaat atgaattcgg cggtgcgctg aacggcttca gcctcggcgg
     6181 ctcgctccgc tgggaaagcc ggccgccgca gacggcgccc aatcccgtga ccgaacagga
     6241 tgaaccggtt gggcaaaagg cctatgctct ggtgaacctg atgggagctt atgacctgac
     6301 cgaagccgtg tcgctccagg tcaacgtcaa caatgtcttc gacaaagcct attacaacac
     6361 caatagctgg ttcggcggct tcatctatgg cgagccgcgt aatgttcggg tgacgttgcg
     6421 ctacgggttt tgatcgcgaa atagctgccg cctggcgcgg ggccgcgaca ggggccccgg
     6481 tagcgcgcga gcgtgctcga gcacgcgcgc cgtggcggcg tatctctctg ctttcgccaa
     6541 tgcgccgcgc gggggcgaag cggccggccc tgcgccggcc cctgtcctag agacgatggc
     6601 gatgcctgcc gcgcatgata tcgcgccgtc gcgcccgctc gcgcctgaac cgggcgatga
     6661 gatagattgc ggcgcccacc atgacgacga tgatggcata ggcgacaagg attctcggtt
     6721 ccacggtggt tactccggcc tagaatgagc gagaccccgc accgatacgc tcgccgttcc
     6781 gttaggttcc gtcccggggg tcgggaagcc ggccgccgtt tccccgatct ctcgatataa
     6841 ccatatgggt taaaataact tgacgtcgtt acgctgatat ggcacaagtg tcacatcatg
     6901 aagaattgcg aatcgggccg ggggccttcc gttttgcggg agcctttcgg cccggatctt
     6961 ttcgggagac gggcgatggg acggaacggc aaggcggcgg aaggttccgg taatgcacgc
     7021 ccgggccggc ggcagttgga cattttcctc gagtcgctcg ccgaaagctc caatgttgcc
     7081 gcttcggccc gcgcggcgga gatttcggcg aacgccatgt accgcgagcg gcggcgcaat
     7141 cccggcttcg cggcgcgctg gcacgaggcg ctgtgcgagg gcttcgcgcg gctcgaagcc
     7201 gagctgttgt ccgaggcatt gatcgcgccc tcgggcaatg tgaaggacgc gacgctgaag
     7261 tcgcgggccc agaaatatcg gctcggcctt tcgctgctcg cggcgcaccg ggcggcggtg
     7321 cggggtgcaa ggctccccgc tgccggtgcg atgcctaagg gcggcgcaaa ggcaaggctc
     7381 atcgcgaagc tggacgccat gcgcgcgcag gtggccgcag agaatgccga agaaggcctg
     7441 ctttgaagga tcttgccgaa ctgctgacca tgccgccggc cgaatatgcg gcgtgggcgc
     7501 accggcttcg cgacgacaag gcggcggagc tgtggaccga ttggtcctgg tggcgtcgcg
     7561 gcgatcaatg cccgcccgcg ggcgactggc atgtctggat gttgctcgcc gggcgcgggt
     7621 tcggcaagac gcggacgggc gccgaatgga tacgcggcta tgccgaaacc catccgggcg
     7681 cgcggatcgc gttggtcgcg gcgtcgctgc acgaagggcg gcaggtgatg atcgagggcg
     7741 agagcggcct gctcgcgatc gcgcccgatg ataaccgccc cgattatgaa agcagtcttc
     7801 gccggctgag ctgggccaac ggggcggtgg cgacgctcta ttcggcggcg gagcccgaga
     7861 gccttcgcgg ccccgaacat tcggccgcct ggtgcgacga gatcgccaaa tggccgcagg
     7921 gcgaggcggc gtgggacaat ctgatgctga cgatgcggat cggcgataac ccgcgcgtcg
     7981 tcgcgacgac gacgccgcgc ggggtaccct tggtgcggcg tttgatcgac gagaagggcg
     8041 tcgaaacgac cggcgggagc acgcggagca atcgccgcaa tctgtcgccg caatggctgg
     8101 cgacgatgga caggctttat ggcggcacac ggctcgggcg gcaggaactg ggcggcgaat
     8161 tgctcgagga tgtcgagggc gcgctgtgga cgcgggcgct ggtcgagcgg tgccgggtcg
     8221 aggccgatgc tacggccaag ccggtgcgcg tggtgatcgg cgtcgatccg ccggcgacgg
     8281 cgaacggcga cgcgtgcggg atcgtggtcg cggt
//
LOCUS       DATMSU010000550         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_18407, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000550 DATMSU010000000
VERSION     DATMSU010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18407"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /gene="galE"
                     /locus_tag="VN034_14730"
     CDS             complement(<1..957)
                     /gene="galE"
                     /locus_tag="VN034_14730"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWV61916.1"
                     /translation="MNILLTGGAGYIGSHVAAPLVSAGHRVVCFDNLSNSDPAVMDRL
                     EAITGHAVPLITGDIRDGDALRQVMQDHKIEAVIHFAGLKAVGESVAEPMKYYDNNVR
                     GTLSLLEAMADCGAKTLVFSSSATVYGQPQYLPLDESHPTSATNPYGRTKLMIEEMLA
                     DVAAADPEWRIAILRYFNPVGAHDSGLIGENPNGIPNNLMPFVSRVAAGRLAELSVFG
                     NDYDTPDGTGVRDYIHVVDLAEGHVAALGAIAESDQPLSIWNLGTGQGYSVLDMVEAF
                     ERVNGVRVPYRIAPRRDGDVASCFASPDRAARELGWKAERGLD"
     gene            1124..1420
                     /locus_tag="VN034_14735"
     CDS             1124..1420
                     /locus_tag="VN034_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HWV61917.1"
                     /translation="MTNALKLSLAASAVLAVAAGTAIGGTAVAAEGAKEKCYGVALKG
                     KNDCAAGPGTSCAGTSTVDYQGNAWKHVPAGSCVKMGGTLTAHKGNAKPVAKKG"
     gene            1427..2290
                     /locus_tag="VN034_14740"
     CDS             1427..2290
                     /locus_tag="VN034_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HWV61918.1"
                     /translation="MTAPLPSPLPARAGIGLKPQHYADVLAAAGQGTAPAWVEVHPQN
                     YFGAGGPPHRWLTAIAERLPLSFHSVGLSLGSAAGVDRDELAALAALCRRYAPAMVSD
                     HLSWSNGPDDKFPDLLPVPYSHAALDHFTVEVGRVQDRLLRPMLIENPSRYLAYAGDD
                     WGEVDFLHELCRRSGCGLLLDINNVEVSAFNLGLDPAAWLDAFDPRHVGEIHVAGHAV
                     KDDDMGGTIAIDDHGSPVRASCWDLLARFLDRSGPKPVLIEWDSDVPDYATLIAEAAK
                     ADALLRAPANA"
     gene            2283..2981
                     /locus_tag="VN034_14745"
     CDS             2283..2981
                     /locus_tag="VN034_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HWV61919.1"
                     /translation="MLEQGQAAIAATLLRGPGHLPPDLFIGSDAAVLRGLRVHANTIS
                     HARLIALEETFPRTRDYLGEGEFNLLSRRFVDEGGAGRRSLNAIGGGFADWLADPRAA
                     DLARSEWAWLESYHAADAPALALTDLIGLGETELLALPVRRHPAVRIVALTGDAAPLV
                     DPAFAADTRVLLVTRPDADVRLCGAEPSDATALDMAENIVPVGNLIAHLAEQHPDGGA
                     AIAALIEAGAFERV"
     gene            2985..3443
                     /locus_tag="VN034_14750"
     CDS             2985..3443
                     /locus_tag="VN034_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWV61920.1"
                     /translation="MNAITGFYDKGVAVAGSRFFEGVALFLLRIALAGVFWRSGQTKV
                     VEGSFLTIDPSQYDLFRSEFSGLPLDPAIAVPLTTFSEHLFPALLLVGLATRLSAGAL
                     LVMTLVIQIFVFPDAWWPVHSLWAAMAAILIVRGGGLFSLDALIARIRAQ"
     gene            3440..3961
                     /locus_tag="VN034_14755"
     CDS             3440..3961
                     /locus_tag="VN034_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWV61921.1"
                     /translation="MSIDEPSLARLMAASQRGDRSAYRALLVDCRKWLERYFARRIAP
                     HHIDDLVQETLVSMHRKLATWDSGRAFLPWLAAIARYRWIDMLRRQRDEAELGDNDAA
                     VGAEDEAVHARLSIDHLLTLLPPKQAQVITLVKIEGASIAEASQICGQSESLVKVNIH
                     RGLKKLAELIESE"
     gene            3964..>4415
                     /locus_tag="VN034_14760"
     CDS             3964..>4415
                     /locus_tag="VN034_14760"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HWV61922.1"
                     /translation="MKDASIDDLIDGLASDLKSVRRRRVTRGSLWVAAGWLGVGALLI
                     GLFGARHNLAAGAMPPLSMLAFWLIATAGIAAAWSALCMGLLGVGRDYGGWRWAVGAL
                     LALPLAALFVFFGDEHGVEAAQRGFDIHCLLQGVVSGLGVGAALFLWL"
BASE COUNT          680 a         1423 c         1489 g          823 t
ORIGIN      
        1 gtcgaggccg cgctcggcct tccagcccag ttcgcgcgcg gcgcggtctg ggctggcgaa
       61 gcagctcgcg acgtcgccat cgcggcgcgg ggcgatgcga taagggacgc ggacgccgtt
      121 cacgcgttcg aaggcctcga ccatgtcgag caccgaatag ccctgaccgg ttccgaggtt
      181 ccagatcgac agcggttgat cgctctccgc gatcgcgccc agcgccgcga catggccctc
      241 ggcgaggtcg acgacgtgga tatagtcgcg cactccggtg ccatcgggcg tgtcatagtc
      301 gttgccgaac accgatagtt cggcgagccg tccggcggcg acgcggctga cgaagggcat
      361 cagattgttc ggaatgccgt tgggattctc gccgatcagc ccgctgtcgt gcgcgccaac
      421 ggggttgaaa tagcgcagga tggcgatgcg ccattcggga tcggcagcgg cgacatcggc
      481 gagcatctcc tcgatcatca gcttggtgcg gccataggga ttggtcgccg aggtcgggtg
      541 actctcgtcg agcggcagat attgcggctg gccatagacc gtcgcgctcg acgaaaagac
      601 gagcgtcttg gccccgcaat cggccatcgc ttcgagcagc gacagggtgc cgcgcacatt
      661 attgtcgtaa tatttcatcg gctcggcgac cgattccccg acggccttca gccccgcgaa
      721 atggatcacg gcctcgatct tatggtcctg catcacttgg cgcagcgcgt cgccgtcgcg
      781 gatgtcgccg gtgatgagcg ggacggcatg gccggtgatc gcttccagcc ggtccatcac
      841 ggcggggtcg ctgttcgaca gattgtcgaa gcagacaacg cggtgcccag ccgacaccag
      901 cggcgcggca acatggcttc cgatatagcc ggcgccgccc gtcagcagaa tattcatgcc
      961 ttgcccgtct ccattcgtgc gtcgttgttc gaccggaggc ctattctatc gggatcgtcc
     1021 tgccaaatcc ttgtctcccg cctcgacgta actttcgcac cttctcccgc gaactcgtga
     1081 cgatgccatc ctccgcatcg cccattttcg aagggatata tccatgacca atgcactgaa
     1141 actgtcgctc gccgcttcgg ccgtcctcgc cgtggccgcg ggcaccgcga tcggcggcac
     1201 cgccgtcgcc gccgagggcg ccaaggaaaa atgctatggc gtcgcattga agggcaagaa
     1261 cgactgcgcc gccgggccgg gcacgagctg cgccggcacc tcgaccgtcg attatcaggg
     1321 caatgcctgg aagcatgtgc ccgccggcag ctgcgtcaag atgggcggca cgctgaccgc
     1381 gcacaagggc aatgccaagc cggtcgccaa gaagggctga ccgcgcgtga ccgctccctt
     1441 gccttcgccg ctgcccgcac gcgccggtat cgggctcaag ccgcagcact atgccgatgt
     1501 gctggcggcg gcggggcagg ggacggcgcc cgcctgggtc gaagtccacc cccagaatta
     1561 tttcggcgcg ggcgggccgc cgcaccgctg gctgacggcg atcgcagagc ggctgccgct
     1621 cagcttccat tcggtcgggt tgtcgctggg ctcggccgct ggtgtcgacc gggacgaact
     1681 cgccgcgctc gccgcactct gtcgccgcta tgcgccggcg atggtctccg accatctcag
     1741 ctggagcaac gggcccgacg acaagtttcc cgacctgctg ccggtcccct atagccacgc
     1801 ggcgctcgat catttcacgg tcgaggtcgg ccgcgtgcag gaccggctgc ttcggccgat
     1861 gctgatcgaa aacccctcgc gctacctcgc ctatgccggc gacgactggg gcgaggtcga
     1921 tttcctgcac gagctttgtc ggcgcagcgg ctgcggcctg ctgctcgata tcaacaatgt
     1981 cgaggtttcg gcgttcaatc tcgggctcga tcccgcggca tggcttgacg ccttcgaccc
     2041 gcgccatgtc ggcgaaattc atgtcgcggg ccatgcggtc aaggacgacg atatgggcgg
     2101 gacgatcgcg atcgacgacc atggatcgcc ggtgcgcgcg agctgttggg acctgctggc
     2161 gcgatttctc gaccgttccg ggcctaagcc ggtgctgatc gaatgggata gcgacgttcc
     2221 cgattatgcc acgctgatcg ccgaagccgc caaggccgac gcgctgctgc gggcgcccgc
     2281 taatgcttga gcagggacag gcggcgatcg ccgcgaccct gctgcgcgga cccggccatc
     2341 tgccgccgga tcttttcata ggtagcgacg ccgcggtgct gcgcgggctt cgcgttcacg
     2401 ccaacaccat atcgcacgcg cggctaatcg cgctcgaaga gacgttcccg cgcacgcgcg
     2461 attatctggg tgaaggcgag ttcaaccttc tgtcgcgccg gtttgtcgat gaaggcgggg
     2521 ctgggcgccg gtcattgaac gctatcggcg ggggctttgc ggactggctc gccgatccgc
     2581 gcgccgccga cctcgcgcgc tccgaatggg cgtggctcga aagttatcac gccgccgatg
     2641 cgcccgcgct ggcgctcacc gatctgatcg gcctcggcga aaccgaacta ttagcactgc
     2701 cggtccgtcg gcacccggcg gtgcgcatag tcgcgctgac cggcgatgcc gcgccgctcg
     2761 tcgatccggc gttcgcggcc gatacgcggg tactgctcgt cacgcgcccc gatgccgatg
     2821 tccgcctgtg cggggccgag ccttccgacg cgacggcgct cgacatggcg gaaaacatcg
     2881 tgccggtcgg taacctgatc gcgcatctcg ccgaacagca tccggacggc ggcgcggcga
     2941 tcgcggcgct gatcgaggcc ggggctttcg agagggtttg atcgatgaat gcgattacgg
     3001 gattttatga caagggcgta gccgtcgcgg gatcgcggtt tttcgaaggc gtcgcgctgt
     3061 tcctgctgcg gatcgcgctt gcgggcgtgt tctggcgatc ggggcagacg aaggtggtcg
     3121 agggcagttt cctcacaata gacccgtcgc aatatgatct gtttcgcagc gaattttcgg
     3181 ggctgcctct cgatcccgca attgcggttc cgctcacgac cttttccgaa catctgttcc
     3241 ccgcgctgct gctcgtcggc ctcgcgacgc gcctttcggc gggcgcgctg ctcgtgatga
     3301 cgctcgtcat ccagattttc gtctttcccg atgcctggtg gcccgtacac tcgctgtggg
     3361 cggcgatggc ggcgatcctg atcgtgcgcg gcggcgggct gttctcgctc gacgcgctga
     3421 ttgctaggat acgcgcgcaa tgagcatcga cgaaccttcg ctggcgcgct tgatggccgc
     3481 atcgcagcgg ggggatcgca gcgcctatcg cgcgctgctc gtcgattgcc gcaaatggct
     3541 cgaacgctat ttcgcgcggc ggatcgcgcc gcaccatatc gatgatctgg tgcaggaaac
     3601 gcttgtgtcg atgcaccgca agctcgcgac gtgggacagc gggcgggcgt tcctgccctg
     3661 gctcgccgcg atcgcgcgct atcgctggat cgacatgctg cgccgccagc gcgacgaagc
     3721 cgaactgggc gacaatgacg ccgcggtcgg cgccgaggac gaagcggtcc atgcgcggct
     3781 cagcatcgac catctgctga cgctgttgcc gccgaagcag gcgcaggtga tcacgctggt
     3841 caagatcgaa ggcgcgtcga tcgccgaggc gtcgcagatt tgcgggcaga gcgagtcgct
     3901 cgtcaaggtg aacatccatc gcgggctcaa gaagctcgcc gaactgattg aaagcgaatg
     3961 acgatgaagg acgcatcgat cgacgatctg atcgacgggc tggcaagcga tctgaaatcc
     4021 gtgcgccggc ggcgtgtgac gcgcggatcg ctgtgggtcg ccgcgggatg gctgggcgtc
     4081 ggtgcattgt tgatcgggct gtttggagca cggcacaatc tggcggcagg cgcgatgccg
     4141 ccgctttcga tgctcgcctt ctggctcatc gccaccgccg gcatcgcggc ggcgtggagc
     4201 gcgctgtgca tggggcttct tggtgtcggg cgcgattatg gcggttggcg ctgggcggtc
     4261 ggcgcgttgc tggcactacc gctcgcggcg ctcttcgtat tcttcggcga tgagcatggc
     4321 gtcgaagctg cgcagcgcgg tttcgacata cactgcctgc tccaaggcgt ggtttcaggg
     4381 ctgggcgtcg gggcagcgct gtttctctgg ctcag
//
LOCUS       DATMSU010000551         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_37701, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000551 DATMSU010000000
VERSION     DATMSU010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37701"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1487
                     /gene="atpD"
                     /locus_tag="VN034_14765"
     CDS             72..1487
                     /gene="atpD"
                     /locus_tag="VN034_14765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWV61923.1"
                     /translation="MAQVIGAVVDVQFTGPLPAILNALETDNNGNRLVLEVAQHLGEN
                     TVRTIAMDATDGLTRGQPVTDTGAQITVPVGPQTLGRILNVIGDPIDERGPVNAGMSA
                     PIHAKAPEFVDQSTEAAILVTGIKVIDLIAPYAKGGKIGLFGGAGVGKTVLIQELINN
                     IAKGHGGVSVFAGVGERTREGNDLYHEFLDAGVIAKDADGNPTPDGSKVALVFGQMNE
                     PPGARARVALSGLTMAEYFRDQEGQDVLFFVDNIFRFTQAGSEVSALLGRIPSAVGYQ
                     PTLSTDMGALQERITSTTKGSITSVQAIYVPADDLTDPAPATSFAHLDATTTLSRAIS
                     ELGIYPAVDPLDSTSRVLTAATVGQEHYETARRVQETLQKYKSLQDIIAILGMDELSE
                     EDKLVVARARKIQRFLSQPFHVAEVFTGIPGKFVAIEDTVKSFKAVVDGEYDHLPEAA
                     FYMVGGIDEAVAKAAKLAEDA"
     gene            1636..1893
                     /locus_tag="VN034_14770"
     CDS             1636..1893
                     /locus_tag="VN034_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HWV61924.1"
                     /translation="MALKFELVTPARLERSADVHMVTVPGTEGDFSVLEGHAPFMATL
                     RNGPLTIYASQGAEPEVIEVEGGFAEVNEAGLTVLAEHIAG"
     gene            2085..3581
                     /locus_tag="VN034_14775"
     CDS             2085..3581
                     /locus_tag="VN034_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWV61925.1"
                     /translation="MSAFGRRPGTAGRPAFGVAKPMQTGPGVPGGAQFPAIDTAPAEA
                     PPRIPSNLTPEEEAMERLNQRSTAEMAEPEKAQGFEASVHKIKEQVLPRLLERVDPEA
                     AATLSKDELTEEFRPIILEVLAELRITLNRREQFALEKVLVDELLGFGPLEELLADPD
                     ISDIMVNGPYQTYIERKGQLVIAPIQFRDEQHLFQIAQRICNMVGRRVDQTTPLADAR
                     LKDGSRVNVIVPPLSLRGTAISIRKFSAKPITLDMLCQWGAMSQKMCTALKIAGASRF
                     NIVISGGTGSGKTTMLNALSKMIDPGERVLTIEDAAELRLQQPHWLPLETRPANLEGN
                     GAIHMGDLVKNALRMRPDRIIMGEVRGAECFDLLAAMNTGHDGSMCTLHSNSPRECLG
                     RMENMVLMGDIKIPKEAISKQIADSVDMIVQIKRLRDGSRRVTNVTEVIGMEGDVIVT
                     QELFKFEYLDEDKDGKIVGEYRAMGLRPYTLEKARQYGFDQPYLEACL"
     gene            complement(3756..3935)
                     /locus_tag="VN034_14780"
     CDS             complement(3756..3935)
                     /locus_tag="VN034_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61926.1"
                     /translation="MSMLSVAIAAATLFGIAEIANWRRNNRRDVDDVGFMPWRGIALA
                     SAAVALFATAFALKP"
BASE COUNT          789 a         1434 c         1264 g          660 t
ORIGIN      
        1 cgcccaaggc tgctgcgccg aagaaggccg ccgcgcccaa ggctgcaagc accggcaccg
       61 ccacgggccg catcgcacag gtcatcggcg ccgtcgtcga cgtccagttc accggcccgc
      121 tgccggcgat tctgaacgcg ctcgaaaccg acaacaacgg caaccgcctc gtcctcgaag
      181 tcgcgcagca cctcggcgag aacaccgtcc gtacgatcgc gatggacgcg accgacggcc
      241 tgacccgcgg ccagccggtg accgacaccg gcgcccagat caccgtgccc gtcggtccgc
      301 agacgctcgg ccgcatcctc aacgtcatcg gcgacccgat cgacgaacgc ggcccggtca
      361 acgccggcat gtcggcgccg atccacgcca aggcccccga attcgtcgac cagtcgaccg
      421 aagccgcgat cctcgtcacc ggcatcaagg tcatcgacct catcgccccc tatgccaagg
      481 gcggcaagat cggcctgttc ggcggcgccg gcgtcggcaa gacggttctc atccaggaac
      541 tgatcaacaa catcgccaag ggccacggcg gcgtttcggt gttcgcgggc gtcggtgaac
      601 gcacccgcga aggcaacgat ctctatcacg aattcctcga cgccggcgtt atcgccaagg
      661 acgccgacgg caacccgacc cccgacgggt cgaaggtggc gctggtgttc ggccagatga
      721 acgagccccc gggcgcccgc gcgcgcgtcg cactctcggg cctcaccatg gccgaatatt
      781 tccgcgacca ggaaggccag gacgtgcttt tcttcgtcga caacatcttc cgcttcaccc
      841 aggcgggttc ggaagtgtcg gcgctgctcg gccgtattcc gtcggcggtg ggttaccagc
      901 cgaccctgtc gaccgacatg ggcgcgctgc aggaacgcat cacctcgacc accaagggct
      961 cgatcacctc ggtgcaggcc atttacgttc ccgcggatga cttgaccgac cccgcgccgg
     1021 cgacctcgtt cgcccacttg gacgcgacga cgacgctgag ccgcgcgatt tcggaactcg
     1081 gcatctaccc cgcggtcgac ccgctcgatt cgacctcgcg cgtgctgacc gccgccacgg
     1141 tcggccagga gcattacgag accgcgcgcc gcgttcagga aacgctgcag aaatacaagt
     1201 cgctgcagga catcatcgcg atcctcggca tggacgagct gtcggaagaa gataagctgg
     1261 tcgtcgcccg cgcgcgcaag atccagcgct tcctcagcca accgttccac gtcgccgaag
     1321 tcttcaccgg catccccggc aagttcgtcg cgatcgaaga cacggtgaag tcgttcaagg
     1381 ccgtcgtcga cggcgagtat gaccatctgc ccgaagcggc cttctacatg gtcggcggca
     1441 tcgacgaagc ggtcgccaag gccgcgaagc tcgccgaaga cgcgtaatct aataccccct
     1501 ccccttcagg ggaggggcag cgagacttgc gagcttgctc gctagtcgca gcggggtggg
     1561 ccacgaggtc cgtgctaggc cgatggcctc accccaaccc ctcccctgaa ggggaggggc
     1621 tttaggaatg aagacatggc tctgaagttc gaactcgtca cccccgcccg cctcgagcgg
     1681 tccgccgacg tccatatggt caccgtgccg gggaccgagg gcgatttttc ggtgctcgaa
     1741 ggccacgcgc ccttcatggc gacgctgcgt aacggcccgc tgacgatcta tgcgtcgcag
     1801 ggcgccgagc ccgaggtcat cgaggtcgaa ggcggcttcg ccgaggtcaa tgaagcgggc
     1861 ctcaccgtgc tggccgagca tatcgccggc tgaattaccc tgccgtcgac ggccgggacc
     1921 aaaagcccgc atcctcgccg atgcgggctt tttcatgtcc gcgcggcaaa cacccgccgc
     1981 attaggacaa tgtcaaagtt tccggtttat tcaccgtgtc ggatgggggt ttcgccggga
     2041 agtcggcggc attcccgcat gagatacgaa gcaggataaa acggatgagt gcattcggac
     2101 gccgccccgg aaccgctggc cgccccgcct ttggcgtggc gaagccgatg cagactggcc
     2161 ccggcgtgcc cggcggcgcg caattcccgg cgatcgacac cgcgcccgcc gaagctccgc
     2221 cgcggatccc gtcgaacctg acgcccgaag aagaggcgat ggagcggctg aaccagcgct
     2281 cgaccgccga aatggccgag cccgaaaagg cgcaggggtt cgaagccagc gtacacaaga
     2341 tcaaggaaca ggtgctgccg cgcctgctcg aacgcgtcga tcccgaggcg gcggcgacgc
     2401 tgagcaagga cgagctgacc gaggaattcc gcccgattat cctcgaagtg ctcgccgagc
     2461 tccgcatcac gctcaatcgc cgcgaacaat tcgcgctcga aaaggtgctc gtcgacgagc
     2521 tgctcggctt cggcccgctc gaggaattgc tcgccgatcc cgacatttcg gacatcatgg
     2581 tcaacggtcc gtaccagacc tatatcgaac gcaagggtca gctggtcatc gcgccgatcc
     2641 agttccgcga cgaacagcat ctgttccaga tcgcgcagcg catctgcaac atggtcggcc
     2701 gccgcgtcga ccagacgacg ccgctcgccg acgcccgttt gaaggacggt tctcgcgtca
     2761 acgtgatcgt cccgccgctg tccttgcgcg gcaccgcgat ctcgatccgt aaattctcgg
     2821 ccaagccgat cacgctcgac atgctctgcc agtggggcgc gatgagccag aagatgtgca
     2881 ccgcgctcaa gatcgcgggc gcgagccgtt tcaacatcgt catctcgggc ggcaccggtt
     2941 cgggtaaaac caccatgctc aacgcgctgt cgaagatgat cgaccccggc gaacgcgtgc
     3001 tgacgatcga ggacgccgcc gaactccgcc tgcaacagcc gcactggctg ccgctcgaaa
     3061 cgcgccccgc gaacctcgag ggcaatgggg cgatccacat gggcgatctc gtcaagaacg
     3121 cgctgcgtat gcgccccgac cgcatcatca tgggtgaggt ccgcggcgcg gagtgtttcg
     3181 accttctcgc cgcgatgaac acgggtcacg acggttcgat gtgtacgctc cactcgaaca
     3241 gcccgcgcga atgccttggc cgtatggaga atatggtgct gatgggcgac atcaagattc
     3301 cgaaggaagc gatctcgaag cagatcgccg attcggtcga catgatcgtc cagatcaagc
     3361 gcctgcgcga cggttcgcgc cgcgtcacca acgtcaccga ggtgatcggg atggaaggcg
     3421 acgtcatcgt cacccaggaa ctcttcaagt tcgaatatct cgacgaggac aaggacggca
     3481 agatcgtcgg cgaatatcgc gcgatgggcc tgcgtcccta tacgctggaa aaagcgcgcc
     3541 agtacggctt cgaccagccc tatctcgagg cgtgcctgta accttcttcc tctccccttg
     3601 ggggagaggg atgcgaaggc ttggcagctt gctgcctagt cgtagctggg tgagggccta
     3661 gacctctcgc cttccaacgg acagaccctc atccaactcc gcctagccgc tccgcggcaa
     3721 ggctccatat ccttctcccc caaggggaga aggaatcagg gcttcaacgc gaacgccgtc
     3781 gcgaacagcg ccaccgccgc cgaggccagc gcgatcccgc gccagggcat gaaaccgacg
     3841 tcgtcgacgt cgcgccggtt gttgcggcgc cagttggcga tctcggcgat gccgaacagc
     3901 gtcgccgccg caatcgccac cgacagcatc gacacttgca ttgccgcgca tcccttcgca
     3961 aacaggggta ttccccccgg tctccgcaag gagacgaatc cctcagcaga gaggtgccca
     4021 tatgcgtgtc ctgtcattgc ttgcaagtgc cgccgccctg atcgccgttc ccgctgcggc
     4081 gatgcagcat gacggccatg ccggtcatgc tcccgccaag agcggcgatg cgatcgccgc
     4141 cgcggtc
//
LOCUS       DATMSU010000552         6148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_104070,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000552 DATMSU010000000
VERSION     DATMSU010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104070"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1533)
                     /locus_tag="VN034_14785"
     CDS             complement(28..1533)
                     /locus_tag="VN034_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HWV61927.1"
                     /translation="MGDKFEIVIVGGGTAGWMCAAACAAKLDPARFRVRLVESEEIGT
                     VGVGEATLPQMKDFNDFLGLDEAEFMQATQATFKLGIEFVNWGRAGDRYVHPFGTFGR
                     PIGEADFFSYWIRAHVAGTAAPLSDYCFPIVTALQNRFMLPSGDANDVRSAFAYAYHL
                     DAFLYARYLRGWAEARGIERVEGRIVEVEQDSDSGDIAAVKLASGTRVAGDLFVDCSG
                     FRSLLLGQTLGVDLEDWAHWLPCDSAQAVPSVRSADFHPYTRSTRRTAGWQWRIPLQH
                     RTGNGYVYSSADISDDEAAATLLAGLDGEALADPRVLRFKAGKRQKAWAHNCIGMGLA
                     GGFLEPLESTSIYLVQMAIMHMLTLMPAERAIDPALPCEFNRVMDVEYDRIRDFLILH
                     YIANEADAPLWERVTAVDVPDTLADKIDRFRHRGHVQAYRDGLFGPPSWQAVFVGQGI
                     EPQAADRLADVLPAATVDERLANLAATIADAAATLPSHADFIARYCPAPAP"
     gene            complement(1602..4844)
                     /locus_tag="VN034_14790"
     CDS             complement(1602..4844)
                     /locus_tag="VN034_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61928.1"
                     /translation="MIKGPANTYRIRHSLLQGICGAALASAMILPAAAQESTVPVSAD
                     DENPDDIVVTGIRANLESAQNRKRNADTVVDSITAEDIGSFPDKSVAEALQRVPGITV
                     IRFAGTDDTSHFSAEPSGVVIRGLNQVRSEFNGRDTFSANSSRGLSWQDISPELLGGI
                     DTYKNQTADLIEGGIAGTVNLRTRVPFDQDGRLISISIKNTYGDIAKKSTPEISGIIS
                     DRWQTGIGEIGLMLSGAYSHAITASQGIQFDRMAIFDGVFGPGKQYIPSGIYMRDNEY
                     DRKRYGVSAAFQWRSNDGDMELTGQYLRSQYNNSWREHAIYSSAFSIYGQPSDYQVTD
                     PTLVNPLRGTDPFTFDDDGNFLSGWWSAPRPYVGEGDANRGLGVNEDGEAFFNRCYSW
                     EVDGNGVPFCDNPQRAPQLDTAANALRNKQVTQDFGLNFRWDVSDRLRVTLDGQYVDA
                     SVNNYNASVTARSYANTFVDLTGKYPRLEFLPNAADNINLSDGGLNNPNNYHYYAVTD
                     HTEDSDGEEVSFRADVEYDVDSGWLDAIKVGARYADRDQTVRWGAYNWANISNTWTYT
                     QAPYFNIDSPAYAPGTNRLHTFDGDFFAGNQINQNSFAFFNMDMLSNREQLAAALGRP
                     SIGVGDYYPVCSGEGYRGAETIEKAFGCYFPSEVHRVSERTIATYAMAKFGGDSLAIG
                     GVPISGNIGVRLIWTRDDTVGATTLPVPFTPTALLCERGTDPGPPPRPTASSGCVVTQ
                     QEIDFSNGATVADTTKVNHFHALPSFNIKFDLTDKLVSRFAYSRAMSRPDFGLLRNFL
                     TVNRLTPNLNDFSDPNVTRDADGNAIAYDWQYTGQSGNPGLKPMTADQFDLTLEYYFG
                     RSSSFTATGFYKKFYDYIQAGQFNLEVTNNGVTEDVRVNRPVNGDGASIYGAEFAFQT
                     FFDFLPAPFDGFGVQANYTYVKNDGIETVNLTNETAGGTAGGGLTYEDSTVKAKALEG
                     ISKHSYNLVGMYEKGPVSARVAYNWRSKYLVTAIDCCVGFPIWQKSTGYLDASLRLRA
                     TANIEFVLEGTNLLGTDTVLMQQVDGDGTLKPNAWFKNDRRYQLGVRLNF"
     gene            5021..6103
                     /locus_tag="VN034_14795"
     CDS             5021..6103
                     /locus_tag="VN034_14795"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HWV61929.1"
                     /translation="MRLISLDVLRGLTVAGMILVNSAAAMKYGAEANVAPILLHVSWD
                     GLTLADLVFPGFLTMVGIAIPFSMRDATFDSAQRRHILGRTGRLLLLGFILSNLYWFT
                     SFASGDWRLFGVLQRIGLVYCACALLFLLTSPRARLIIAAAILALYWPLALLPSLDGL
                     ATDIWVRGHNFVASVDRLLLGNHLYVKGPEGYDPEGILGTLPAIAQGLIGAAIGELLI
                     RRKGPAARQLAMIGGAMLAIGGVWSLAFPIVKDIWSSSFVLVTSGITVLALALLHHWL
                     DREGRKPGVAATAMLAFGANAIAAYTLHQVTAGVVTWDLLLLPFRALRGALGDPVASL
                     LPILLYMLLIWGAMEWLRRKGWIIKI"
BASE COUNT         1053 a         1883 c         2006 g         1206 t
ORIGIN      
        1 ggacgatcac ctgctgcacc ggaccggtca tggcgcgggc gccgggcaat agcgcgcgat
       61 gaaatcggca tgcgagggca atgtcgcggc ggcgtcggcg atcgtcgcgg cgaggttcgc
      121 gagacgctcg tcgaccgtcg cggcgggcag cacgtcggcg agccggtcgg cggcttgcgg
      181 ttcgatcccc tgcccgacga acaccgcctg ccagctcggc ggcccgaaca atccgtcgcg
      241 ataggcctgg acatggccgc gatggcggaa gcggtcgatc ttgtccgcga gtgtgtcggg
      301 gacgtcgacc gcggtgacgc gctcccacaa gggcgcgtcg gcctcgttcg cgatatagtg
      361 gaggatcagg aaatcgcgga tgcggtcata ttcgacgtcc atcacgcggt tgaattcgca
      421 tggaagcgcc gggtcgatcg cgcgctcggc gggcatcagc gtcagcatgt gcatgatcgc
      481 catctggacg agatagatgc tcgtcgattc gagcggttcg aggaagccgc ccgcgagccc
      541 catgccgatg caattatgcg cccacgcctt ttgccgcttg cccgccttga agcgcagcac
      601 gcgcggatcg gcgagcgcct cgccgtcgag acccgcgagc agcgtcgccg ccgcctcgtc
      661 gtccgaaatg tcggcgctcg aatagacata gccgttgccg gtgcggtgct ggagcgggat
      721 gcgccattgc cagcccgccg tgcgccgcgt cgagcgggtg tagggatgga aatcggccga
      781 acgcaccgag ggcaccgcct gcgcgctgtc gcacggcagc cagtgcgccc aatcctcgag
      841 atcgacgccg agcgtctgtc cgagcagcag cgagcggaag cccgagcaat cgacgaacag
      901 atcgcccgcg acgcgcgtgc cggaggcgag ctttaccgcg gcgatgtcgc cgctgtcgct
      961 atcctgctcg acctcgacga tccgcccttc gacgcgctcg atcccgcgcg cctcggccca
     1021 accgcgcaga tagcgggcgt agagaaaggc atcgaggtga taggcgtagg cgaaggcact
     1081 ccgcacgtcg ttcgcgtcgc cgctcggcag catgaagcgg ttctgcagcg cggtaacgat
     1141 cgggaagcaa taatcggaga gcggcgcggc ggtgccggca acgtgcgcgc ggatccagta
     1201 gctgaagaaa tcggcctcgc cgatcgggcg gccgaaggtg ccgaaagggt ggacatagcg
     1261 gtcgcccgcg cgcccccagt tcacgaactc gatgccgagt ttgaaggtcg cctgcgtcgc
     1321 ctgcatgaat tcggcttcgt cgagcccgag gaagtcgttg aaatccttca tctgcggcag
     1381 cgtcgcttcg ccgacgccga cggtgccgat ctcctcggat tcgacgaggc ggacgcggaa
     1441 gcgcgcgggg tcgagcttgg cggcgcacgc cgcggcgcac atccaccccg cggttccgcc
     1501 gccgacgatt acgatttcga atttatcccc catcgcgggt ccagcttagc gcggaagaaa
     1561 gcgggcgcca gccccaaagg accgacgccc cttcccccct tttagaagtt gagccgcacg
     1621 ccgagttgat agcggcggtc gttcttgaac caggcgttcg gcttgagcgt tccgtcgccg
     1681 tcgacctgct gcatcagcac ggtgtcggtg ccgagcagat tggtgccctc cagcacgaac
     1741 tcgatattcg cggtcgcgcg gaggcggagc gaggcgtcga gatagcccgt gctcttttgc
     1801 cagatcggga agccgacgca gcagtcgatc gcggtcacca gatatttgga gcgccagttg
     1861 taggcgacgc gcgccgatac cgggcctttc tcatacatgc cgacgaggtt gtagctgtgc
     1921 ttcgaaatgc cttcgagcgc cttggccttt accgtactat cctcataggt aaggccgcca
     1981 cccgccgttc cgccggcggt ttcgttggtc aaattgaccg tctcgatccc gtcgttcttc
     2041 acataggtgt agttggcctg caccccgaag ccgtcgaacg gcgccggcag gaagtcgaag
     2101 aaggtctgga aggcgaattc ggcgccatag atgctcgcgc catcgccgtt gaccggccgg
     2161 ttgacgcgga cgtcctcggt caccccgttg ttcgtcactt caaggttgaa ttggcccgcc
     2221 tgaatataat cgtagaactt cttgtagaag cctgtcgcgg tgaacgaact cgaccggccg
     2281 aaataatatt cgagcgtcag gtcgaactgg tcggccgtca tcggtttgag acccgggttg
     2341 cccgattggc cggtatattg ccagtcatag gcgatcgcat tgccatccgc gtcgcgcgtg
     2401 acattcgggt ccgaaaaatc gttcaggttc ggcgtcagcc ggttcaccgt caggaagttg
     2461 cgaagcaggc cgaagtccgg ccgcgacatc gcccgcgaat aggcgaagcg cgacaccagc
     2521 ttgtcggtca ggtcgaactt gatgttgaag ctcggcagcg cgtggaaatg attgaccttg
     2581 gtcgtatcgg ccacggtcgc gccgttgctg aaatcaatct cctgctgcgt gacgacgcag
     2641 cccgatgatg ccgtcgggcg gggcggcggg ccgggatcgg tccccctctc gcaaagaagc
     2701 gctgtcggtg tgaagggaac gggcaaggtg gtcgcgccca ccgtatcgtc gcgcgtccag
     2761 atgaggcgga cgccgatgtt gcccgagatc ggcacgccgc cgatggcgag gctgtcgccg
     2821 ccgaacttcg ccatcgcata ggtagcgatc gtgcgctcgc tgacacggtg gacttcgctc
     2881 gggaaatagc agccgaaggc cttctcgatc gtctccgcac cgcgataacc ctcgcccgaa
     2941 cagacgggat aataatcgcc gacgccgatc gacggccgcc cgagcgccgc cgcaagctgt
     3001 tcgcgattcg acaacatgtc catgttgaag aaggcgaagc tgttttgatt gatctggttt
     3061 ccggcgaaga aatcgccgtc gaaggtgtgg agccggttgg tgcccggcgc ataggcgggg
     3121 ctgtcgatgt tgaaataggg tgcctgcgta taggtccagg tgttggagat attcgcccag
     3181 ttgtacgcgc cccagcgcac cgtctgatcg cggtcggcgt agcgcgcgcc gaccttgatc
     3241 gcgtcgagcc agcccgaatc gacgtcatat tcgacgtcgg cgcggaagga gacctcctcg
     3301 ccgtcgctgt cctcggtgtg atcggtgacc gcgtaatagt gataattgtt ggggttgttc
     3361 agcccgccgt ccgacagatt gatgttgtcg gcagcgttcg gcaggaattc cagccgcgga
     3421 tatttgccgg tcaggtcgac gaaggtgttg gcataactcc gcgcagtgac cgaagcatta
     3481 tagttgttga cgctcgcgtc gacatattgg ccgtcaagcg tgacgcgcag ccggtcgctg
     3541 acgtcccagc ggaagttcag accgaaatcc tgcgtgacct gcttgttgcg cagcgcgttg
     3601 gcagcggtgt cgagttgcgg cgcacgctgc gggttgtcgc agaacggaac accgttcccg
     3661 tccacttccc atgaataaca gcggttgaag aaagcctcgc catcctcgtt gacgccgagc
     3721 cctcggttgg cgtcgccttc gccgacatag gggcgcggcg ccgaccacca gccgctgagg
     3781 aaattgccgt catcgtcgaa ggtgaaggga tcggttcccc gcagcggatt gaccagcgtc
     3841 ggatcggtga cctgatagtc gctcggctgg ccgtagatgc tgaacgcgct cgaatagatc
     3901 gcatgctcgc gccagctgtt gttgtactgc gagcgcagat actgaccggt cagttccatg
     3961 tcgccgtcgt tcgagcgcca ctggaaggcg gccgagacgc cgtagcgttt gcggtcatat
     4021 tcattgtcgc gcatgtagat gccgctcggg atatattgct tccccgggcc gaacacgccg
     4081 tcgaagatcg ccatacggtc gaattgtatg ccctggctcg cggtgatcgc atgcgaatag
     4141 gcaccgctga gcatcagccc gatctcgcca atccctgtct gccagcgatc cgaaatgatg
     4201 cccgagattt cgggcgtcga tttcttggcg atgtcgccat aggtattttt gatcgaaatc
     4261 gagatgagcc gcccgtcctg gtcgaacggc acgcgcgtgc gcaggtttac cgtgcccgcg
     4321 atgccgccct cgatcagatc ggcggtctgg ttcttgtaag tgtcgatgcc gcccagaagt
     4381 tcgggtgaga tatcctgcca gctgaggccg cgcgagctgt tcgcgctgaa agtgtcgcgg
     4441 ccgttgaatt cggaacgcac ctggttgagg ccgcggatga caacccccga cggctcggcg
     4501 gaaaagtgcg aggtgtcgtc ggtgcccgca aagcggatga cggtgatgcc ggggacgcgc
     4561 tgaagcgctt cggcgaccga tttgtccggg aaggagccga tatcctctgc cgtgatcgaa
     4621 tcgacgacgg tgtcggcgtt gcgcttgcgg ttctgcgccg attcgaggtt ggcgcggatg
     4681 ccggtgacga cgatgtcgtc gggattttcg tcgtccgccg ataccggcac ggtgctttcc
     4741 tgcgccgcgg cgggaaggat catcgcgctc gccagtgccg ccccgcaaat cccctggagc
     4801 aggctgtggc gaatccgata tgtgttggca ggccccttga tcattttatt tttcccccat
     4861 ccaatccggt ccagattgcc ttcccacaat ctctataaga ccaattgcgt ccttggacta
     4921 ttaccaatag actacaaatc caacgcgctg tccattgcaa atttatggca gtgtcaagat
     4981 gccgcccgaa ggggcgctgg cgaccggggg aggaaaagcc atgcgattga tttcactcga
     5041 cgtgctgcgg gggctgaccg tcgccgggat gatattggtg aattcggcgg cggcgatgaa
     5101 atatggcgcc gaggcgaatg tcgcgccgat cctgctgcat gtgagttggg acgggctcac
     5161 tctcgccgac ctggtttttc cgggctttct gacgatggtc gggatcgcca tccccttttc
     5221 gatgcgcgat gcgacgttcg attcggcgca gcgccggcat atcctcggcc gcactggccg
     5281 cctgctgctg ctcggtttca tcctcagcaa tctttactgg tttaccagct tcgcatcggg
     5341 cgactggcgg ctgttcggcg tgctccagcg aatcgggctg gtttattgcg cctgcgcgct
     5401 gctgttcctg ctcacgagcc cgcgcgcgcg cctgatcatc gcggcggcga ttctcgcgct
     5461 ttactggccg ctggcgctgc tcccgtcgct cgacgggctg gcgaccgaca tatgggtgcg
     5521 cgggcataat ttcgtcgcat cggtcgaccg gctgctgctc ggcaaccatc tctatgtgaa
     5581 ggggcctgaa ggctatgatc ccgaggggat attgggcacg cttccggcca tcgcccaagg
     5641 gttgatcggg gccgcgatcg gcgagctgct gatccgccgc aagggccccg cggcgcggca
     5701 gctcgcgatg atcggcggcg ccatgctggc cattggcggc gtctggagtc tcgccttccc
     5761 gatcgtgaag gacatctggt cgagcagctt cgtactcgtc accagcggca tcaccgtcct
     5821 cgcgctcgcc ttgctccatc actggctcga ccgcgaaggc cgcaagcccg gggttgccgc
     5881 gaccgcgatg ctggcgttcg gcgccaatgc gatcgcggcc tatacgctgc atcaggtgac
     5941 ggcgggcgtg gtgacatggg acttgctgct gttgccgttc cgcgcgctgc gcggggcgct
     6001 gggcgatccg gtcgcgtcgt tgctccccat ccttctctat atgctgctga tctggggcgc
     6061 gatggaatgg ctgcgccgca agggttggat cattaaaatc tgaccccgcc caaacatacc
     6121 tcttgatgag acctatataa cctatata
//
LOCUS       DATMSU010000553         5312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_84776, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000553 DATMSU010000000
VERSION     DATMSU010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84776"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VN034_14800"
     CDS             complement(<1..690)
                     /locus_tag="VN034_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HWV61930.1"
                     /translation="MPAEWAPHDAVWIGFPHLAEEWAGAIDEGRRDVAAFANAVHDGG
                     RGEEVRLVVNDLAQADIAAALVDPGVKILIQPLGDIWLRDTGPIIAGTGAARRARNFK
                     FNWWGEKFVMPGDQEIGAALAVESGLPVDDQDWVLEGGGIDVDGTGLCVTTEECLLNL
                     NRNPSLTREDIAVRLHQSLGIDRLLWLGNGLVGDHTDGHVDNLARFVGEGRLALPVAA
                     GADDPNAHIYAD"
     gene            complement(905..2587)
                     /locus_tag="VN034_14805"
     CDS             complement(905..2587)
                     /locus_tag="VN034_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HWV61931.1"
                     /translation="MSRLSVPLALAALLSLAACNSGEASKSAPIAIPDVTIPELSLAT
                     LQEVTKELSSDAYEGRAPGTAGEEKTVAYIIKKYQEAGLKPGNNGKWTQDVPLVEITA
                     KNATPLTFTGGKTPVTAQYAKDYVAFSYRVQPRTEVKDSDVVFVGYGINAPEKGWNDY
                     AGLDVKGKTVVVLVNDPDWQTKEAKGEFNGRAMTYYGRWSYKYEEAARQGAAAVLIVH
                     DTEPAAYGWNVVESSNTGTQYLADSKNGGADQTVANGWIQLPKAKELFASAGQDFDKL
                     SEAAKKKGFKPIALTGVKANFSFDNEISKKMSRNVIGVLPGAKRPDEYVLYTGHWDHL
                     GRCTPVDGDDICNGAVDNASGIAGLVTLAQAFNKAGAPDRSIVFLAVTAEESGLLGSK
                     YYAENPIFPLAQTVGGVNMDALNASGPAKDIVVVGAGKSELDAYVEKLAKMAGRTVKA
                     EPTPEKGFYYRSDHFSFAKLGVPMFNFGSGDDLVEGGVDAGKKAAEDYEKNRYHAPGD
                     EYEAITNWDGMMSDLRLYYAAGRMLAMNEAWPNWVEGDEFRAARDKSRAGAK"
     gene            2773..4755
                     /locus_tag="VN034_14810"
     CDS             2773..4755
                     /locus_tag="VN034_14810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWV61932.1"
                     /translation="MTVGGILSASLSVLALAALGAVGANAQAGTDNAARFGALESIRD
                     ISLSPDGDHLAFIRQGANRTRRLYVVDTAEGAQPREILASDGGEGGNLSWCGWVTDAR
                     LACQLFYRDEVIGEILSATNIFAIDAAGGNVRMLSNRRSHRALYADLRGGSVIDWSST
                     DNGSLLMTRSYVPEGDLRTRIVKRAAGLGVDRVDSVSGAASRVEAANPNAVLYLSDGK
                     GNVRVMATQSYLSTTGQLDPTIRFFYQPKEGGGWEPLAQTDIVTEEGFWPAAVDAATN
                     RVYGFQKIDGRKAVVAMALDGTGTTSTIFAHPEVDVDDIMRVGRNGRIVGVSYATDQR
                     EAVMTDAGIAKMTGALSQALGGKQVVIVDASADESRYLIRAESDIDPGTYYLFDPKAK
                     KLRPLLASREPLANIILSPVKPVSYRAADGTMVPGYLTLPPGRKDAKGLPAIVMPHGG
                     PSARDEWGFDWLAQYFAQSGYAVLQPNYRGSSGYGDKWYQNNGFQSWKTAVSDIADGG
                     RWLVSSQGADSAKLSIVGWSYGGYAALQSGVLAPDLFKAIVAIAPVTDLQQLKDEASR
                     EGGGRINAQFVGTGPHIREGSPAQNADAIKAPVLMFHGTFDQNVDVAQARTMRRALED
                     KGKRVELIEYPELAHSLETGEARTDMLRKISAFLPH"
     gene            complement(4814..>5312)
                     /gene="rpsD"
                     /locus_tag="VN034_14815"
     CDS             complement(4814..>5312)
                     /gene="rpsD"
                     /locus_tag="VN034_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWV61933.1"
                     /translation="QRRKGKMSDFGIQLRAKQKLKGYYGDITEKQFKKNYFEASRMKG
                     DTGQNLIGLLERRLDAVVYRSKFTPTIFSARQLVSHGHVYVNGVKCNIASRLVKPGDE
                     VTLGKKAQEMALVAEAQSLPERDLPEYLAIDGTKATFVRVPTLDEVPYPVKMEPNLVV
                     EFYSR"
BASE COUNT          918 a         1624 c         1723 g         1047 t
ORIGIN      
        1 gtcggcatag atatgggcgt tggggtcgtc tgcgcccgcc gcgacgggca gcgcgaggcg
       61 gccttcgccg acgaagcgcg cgagattgtc gacatggccg tcggtgtgat cgccgacgag
      121 gccattgccg agccacagca gccggtcgat gccgagcgac tggtgcagcc gcaccgcgat
      181 atcctcgcgc gtcagcgacg gattgcggtt gaggttgagc aggcattcct cggtggtgac
      241 gcagaggccg gtaccgtcga cgtcgatgcc gccgccctcg agcacccaat cctgatcgtc
      301 gacgggcagg ccgctctcga cggcgagcgc ggcgccgatt tcctggtcgc ccggcatgac
      361 gaacttctcg ccccaccagt tgaatttgaa gttgcgcgcc cgccgcgccg cgccggttcc
      421 cgcgatgatc ggtcccgtat cgcgcaacca gatgtcgccg agcggctgga tgaggatttt
      481 cacgcccgga tcgacgagcg cggcggcgat gtccgcctgc gccaagtcgt tgaccacgag
      541 ccgcacctct tcgccgcggc cgccgtcgtg gacggcgttg gcgaaagcag cgacgtcgcg
      601 ccggccttca tcgatcgcac ccgcccattc ctcggcgagg tgggggaagc caatccagac
      661 ggcgtcgtgc ggcgcccatt cggcgggcat cgtcaatgaa cccacatggc gtctcccatc
      721 aacaggcggt tatggcgttt acaggcatcg ctggcctcgc tcctttttcg tcatgctgaa
      781 ctcgtttcag catccatggc ctgccttatc ggccaaagct gcgctctatt tgaggacagg
      841 ccatggaccc tgaaacaagt tcagggtgac gaggaagaga gggcggcgat agataggacc
      901 gtccctattt cgccccagcg cgcgacttgt cgcgggcggc gcggaattcg tcgccctcga
      961 cccagttcgg ccacgcttcg ttcatcgcca gcatccggcc cgcggcataa tagaggcgca
     1021 ggtccgacat catgccgtcc cagttggtga tcgcctcata ttcgtcgccc ggggcgtgat
     1081 agcggttctt ctcataatct tcggccgctt tcttgccggc gtcgaccccg ccttcgacca
     1141 gatcgtcgcc gctgccgaag ttgaacatcg gcacgccgag cttggcgaag ctgaaatggt
     1201 ccgaacgata gtaaaagccc ttttcggggg tcggttcggc cttgaccgtg cggcccgcca
     1261 tcttggcgag cttttcgaca taggcgtcga gttcggactt gccggcgccg acgacgacga
     1321 tgtccttcgc cgggccgctg gcattgagcg cgtccatgtt cacgccgccg accgtttgcg
     1381 cgagcgggaa gatcgggttt tcggcataat atttcgaacc gagcaggccc gattcctcgg
     1441 cagtcacggc gaggaagacg atgctgcggt cgggcgcgcc cgccttgttg aaggcctgcg
     1501 ccagcgtcac gaggcccgcg atgccgctcg cattgtcgac cgcgccgttg cagatgtcgt
     1561 cgccatcgac cggcgtgcag cggccgaggt ggtcccagtg gccggtgtag agcacatatt
     1621 cgtcgggacg cttggcgccc ggcaggacgc cgatgacgtt gcgcgacatc ttcttcgaaa
     1681 tctcgttatc gaagctgaaa ttcgccttca cgccggtcag cgcgataggc ttgaagccct
     1741 tcttcttggc ggcttcgctc agcttgtcga aatcctgtcc ggcgctcgcg aacagttcct
     1801 tcgccttggg aagctggatc cagccgttgg cgacggtctg gtcggcgccg ccattcttgc
     1861 tgtcggccag atactgcgtg ccggtgttgc tcgattcgac gacattccag ccataggcgg
     1921 cgggttcggt gtcgtggacg atgagcacgg cggcggcgcc ctgtcgcgcg gcctcctcat
     1981 atttgtacga ccaacggcca taataggtca tcgcgcggcc gttgaattcg cccttcgctt
     2041 ccttggtctg ccagtcgggg tcgttgacga ggacgacgac ggtcttcccc ttcacatcga
     2101 gcccggcata gtcgttccag cccttttcgg gggcgttgat gccgtaaccg acgaagacga
     2161 cgtcgctgtc cttgacctcg gtcctcggct gcacacgata gctgaacgcg acatagtcct
     2221 tggcatattg cgcggtgacc ggcgtcttgc cgccggtgaa ggtcagcggc gtcgcgttct
     2281 tcgcggtgat ttcgaccagc ggcacgtcct gcgtccactt gccgttattc ccgggcttca
     2341 gccccgcttc ctgatacttc ttgatgatgt aggcgacggt cttttcctcg cccgccgtgc
     2401 cgggcgcgcg gccctcatag gcgtccgacg acaattcctt cgtcacttcc tgaagcgtcg
     2461 cgagcgacag ttcggggatg gtaacgtcgg ggattgcgat gggagcactc ttggacgctt
     2521 cgccgctgtt gcaggcggcg agggacagaa gcgcggcgag cgccagcgga acggacaagc
     2581 gggacatgaa gaatccttgc tgatttcgga atgccgggcg ttgtgccagc agcgagcgac
     2641 ggatgcaagg aaggccgggc aggagtcgat cgatggcgat tggagcgcca tttcctgtcc
     2701 agattccaaa catgcttgaa aaatctgagg ttgcccccac tctgccaccg ggaactcagg
     2761 gggagggaat aagtgactgt tggtggaata ttgtctgcgt cgctgtcggt gttggcgctg
     2821 gccgcgctag gagcggtcgg cgcgaatgcc caggccggga cggacaacgc ggcgcggttt
     2881 ggtgcgctcg aatcgattcg tgatatcagc ctgtcgcccg acggggatca cctggcgttc
     2941 atcaggcagg gagccaaccg cacgcggcga ctgtacgtcg tcgataccgc cgagggtgcc
     3001 caaccgcgcg agatattggc aagcgacggc ggtgagggcg gcaatctcag ctggtgcggt
     3061 tgggtgaccg atgcgcggct cgcctgccag cttttctatc gcgacgaagt tatcggcgaa
     3121 atcctgtcgg cgaccaacat ctttgcgatc gatgccgcgg gcggcaatgt ccgcatgctc
     3181 agcaatcgcc gcagccatcg cgcgctttat gccgatttgc ggggcggcag cgtgatcgac
     3241 tggtcgtcga ccgacaatgg ttcgctgctg atgacgcgca gctatgtacc cgaaggcgac
     3301 ctgcgcacgc gcatcgtcaa acgcgccgca gggcttggcg tcgaccgcgt cgattcggtg
     3361 agcggagcag ccagccgcgt cgaagccgcc aatccgaatg ccgttcttta tctgtcggac
     3421 ggcaagggca atgtccgggt catggcgacg cagagctatt tgagcactac cgggcaactc
     3481 gatccgacga ttcgcttctt ttatcagccc aaggaaggag gcggatggga gccgttggcg
     3541 caaaccgata tcgttaccga agagggcttt tggccggcag cggtcgatgc cgccacgaat
     3601 cgcgtttacg gtttccagaa aatcgacggg cgcaaggcgg tcgtggcgat ggcgctcgac
     3661 ggtacgggga cgacgagcac catctttgcg catccggagg tcgacgtcga cgacatcatg
     3721 cgcgttggcc gcaacggccg catcgtcggc gtctcctatg cgaccgatca acgcgaggcg
     3781 gtgatgaccg atgccggcat tgccaagatg accggcgcgc tgtcccaggc cctgggcggc
     3841 aagcaggtcg tcatcgtcga tgcatcggcc gatgaaagcc gctatttgat ccgcgccgaa
     3901 tccgatattg atcccggcac ctattatctc ttcgatccaa aggcgaagaa actgcgtccg
     3961 ctgctggcgt cgcgcgagcc gctggcgaat attatcctgt ctccggtaaa gcccgtatct
     4021 tatcgggctg ccgacggcac gatggtgccc ggctatttga cgctgccgcc gggacggaag
     4081 gacgcaaaag gacttccggc gattgtgatg ccgcacggcg gcccgtcggc gcgcgacgaa
     4141 tggggtttcg actggctggc gcaatatttc gcgcaatcag gctacgccgt actccagccc
     4201 aactatcgcg gatcgtcggg ctatggggac aaatggtatc agaacaacgg ctttcaatca
     4261 tggaagacgg cggtcagcga catcgccgac ggagggcggt ggctggtgtc gagccaaggc
     4321 gcggactccg ccaagctgtc catcgttggc tggtcctatg gcggctatgc agcgttgcag
     4381 tcgggcgtgc tcgcgcccga tttgttcaag gcgatcgtcg cgatcgcgcc ggtcaccgat
     4441 ttgcagcagt tgaaggatga agcgtcgcga gaggggggcg ggcggatcaa cgcccagttc
     4501 gtcggtaccg gcccgcatat tcgcgaagga tcgccggcac agaatgccga cgcgatcaag
     4561 gcgccggtgc tgatgttcca cgggactttc gatcaaaatg tcgacgttgc acaggcgcga
     4621 acgatgcggc gcgcgctgga agacaaaggc aagcgtgttg aattgatcga atatcccgaa
     4681 ctcgcgcaca gcctcgaaac cggcgaggcc cgaaccgaca tgctgcgcaa gatttcggcc
     4741 ttcttgccgc attgaacgaa aaggggcggc ccgttcccgg accgcccctt ttgatttcgt
     4801 gctcgaaacc cgatcagcgc gaatagaatt cgacgaccag attcggttcc atcttcaccg
     4861 gataggggac ttcgtcgagc gtcggaacgc gaacgaaggt cgccttggtg ccgtcgatgg
     4921 cgagatattc gggcaggtcg cgctcgggca ggctctgcgc ttcggcgacc agcgccattt
     4981 cctgcgcctt cttgccgaga gtgacttcgt cgcccggctt cacgaggcgg ctcgcgatgt
     5041 tgcacttcac gccgttcaca tagacgtggc cgtggctgac gagctggcgc gccgagaaga
     5101 tcgtcggcgt gaacttcgag cggtagacga ccgcgtcgag gcggcgctcg agcaggccga
     5161 tcaggttctg accggtgtcg cccttcatgc gcgacgcttc gaaatagttc ttcttgaact
     5221 gcttttcggt gatgtcgccg taatagccct tcagcttctg cttcgcgcgg agctggatgc
     5281 cgaagtccga catcttgccc ttgcggcgct ga
//
LOCUS       DATMSU010000554         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_28235, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000554 DATMSU010000000
VERSION     DATMSU010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28235"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VN034_14820"
     CDS             <1..601
                     /locus_tag="VN034_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWV61934.1"
                     /translation="APADVADRAAIDDAFAAARGTHGPITILVNNAGIAPSAPFARVT
                     AETWRRAMAVNLDALFHCCQSALPDLLAAETGRIVTVASTAGVKGYAYTAPYVASKHG
                     AIGLMRALAAEYAATGLTANAVCPGFTDTDIVGQAVANIRSKTGRSEAEALAELTRFN
                     PQKRLIDPAEVAEAVLWLCLPASRSVTGQAVMIAGGEVM"
     gene            598..1095
                     /locus_tag="VN034_14825"
     CDS             598..1095
                     /locus_tag="VN034_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWV61935.1"
                     /translation="MSERTAIREKHDGALDDRGNVRLWLRLLTCTTVIEKRLKRRFAD
                     QYGITLPRFDVMAALDRHPEGMTMGQLSQALLVSNGNVTGVVQTLLRDRYLNIAPSPT
                     DGRASIVRLTPLGQECFSGLAEAHHEWVDMMLAGLTRDQRTTLFNLLGALKDSLAADN
                     GKEQA"
     gene            1092..1901
                     /locus_tag="VN034_14830"
     CDS             1092..1901
                     /locus_tag="VN034_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HWV61936.1"
                     /translation="MNPESFSPEHFGWHFDGGVATVTLNRPDRKNPLTFESYAELRDT
                     FRALVYAPAVKAIVVTGAGGNFSSGGDVHEIIGPLTEMAMPELLDFTRMTGDLVKAMR
                     ACPQPIIAAIDGICVGAGAIMAMASDMRLATPRAKTAFLFTRVGLAGCDMGACAILPR
                     IIGQGRASDLLYTGRMLGAEEGERWGFHNRLVEPDALLDEATALARSLATGPTFAHGI
                     TKTQLGIEWAVSVETAIEMEAQAQAICMATNDFRRAFEAFADKRTPEFAGD"
     gene            1901..>2665
                     /locus_tag="VN034_14835"
     CDS             1901..>2665
                     /locus_tag="VN034_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWV61937.1"
                     /translation="MADRSFLDWPFLDDGHRALAQDLDAWCAAHLTDHHSDDIDGECR
                     ALVRALGDAGWLRYCIPAAYGGVHDSLDIRSLALIRETLARHSGLADFAFAMQGLGSG
                     TISLFGTEAQKQAYLPDVTAGRKIAAFALTEPASGSDVASMETTATRTADGYVVTGAK
                     TYISNGGIADYYVLFARTGEAPGAKGVSAFIVDADTPGLDVTDRIAVIAPHPLATLTF
                     TDMALADTALLGEAGRGFAQAMATLDIFRTTVGAAAL"
BASE COUNT          437 a          937 c          865 g          426 t
ORIGIN      
        1 cgcccccgcc gacgtcgccg accgcgccgc gatcgatgac gcttttgcag ccgcgcgcgg
       61 cacgcatggg ccgatcacga tcctcgtcaa caacgccggt atcgccccca gcgcgccctt
      121 cgcccgcgtc accgccgaaa catggcgcag ggcgatggcg gtcaatctcg acgcgctgtt
      181 ccattgctgc cagtcggcgc tgcccgacct gcttgccgcc gaaaccggcc gcatcgtcac
      241 cgtcgcctcg acggccggcg tgaaaggcta tgcctatacg gccccctatg tggcgtcgaa
      301 gcatggcgcg atcggcctga tgcgcgcgct cgcggccgaa tatgccgcca ccggcctgac
      361 cgccaacgcc gtgtgcccgg gtttcaccga caccgatatc gtcggacagg cggttgcgaa
      421 catccggtcg aaaaccggcc gcagcgaagc ggaggcgctc gccgagctga cgcgcttcaa
      481 cccgcagaag cggctgatcg acccggcgga ggtcgccgag gccgtgctgt ggctctgcct
      541 tcccgccagc cggtcggtca ccggccaggc agtgatgatc gcgggcggag aagtgatgtg
      601 agcgaacgaa cggccattcg cgaaaagcac gacggcgcgc tcgacgatcg cggcaacgtc
      661 cgtctgtggc tgcggctgct gacctgtacg acggtgatcg aaaagcgcct caaacgccgc
      721 ttcgccgacc aatatggcat cacgctgccg cgcttcgacg tcatggcggc gctcgatcgc
      781 catcccgagg ggatgacgat ggggcaattg tcgcaggcgc tgctggtatc gaacggcaac
      841 gtcaccggtg tcgtgcagac gcttctgcgc gaccgctatc tcaatatcgc gccctccccg
      901 accgatggcc gggcgtcgat cgtccggctg acgccgctcg ggcaggaatg cttcagcggc
      961 ctcgccgagg cgcatcacga atgggtcgac atgatgctgg cgggcctgac gcgcgatcag
     1021 cgcacgacgc tcttcaacct tctcggcgcg ctcaaggatt cgctcgccgc cgacaatgga
     1081 aaggaacagg catgaatccg gaaagctttt cgcccgaaca tttcgggtgg catttcgacg
     1141 gaggcgtcgc gaccgtcacg ctcaaccgtc ccgaccgcaa gaaccccctc accttcgaaa
     1201 gctatgccga gctgcgcgac acctttcgcg cgctcgtcta tgcgcccgca gtgaaggcca
     1261 tcgtcgtgac gggcgccggc ggcaattttt cgtcaggcgg cgacgtgcac gagatcatcg
     1321 ggccgcttac cgaaatggcg atgcccgaac tgctcgattt cacacgcatg acgggcgatc
     1381 tcgtcaaggc aatgcgcgcg tgtccgcaac cgatcattgc cgcgatcgac ggcatctgtg
     1441 tcggcgcggg cgcgatcatg gcgatggcat cggacatgcg gcttgcgacc ccgcgcgcca
     1501 agaccgcctt cctgttcacg cgcgtcggcc ttgccggctg cgacatgggc gcgtgcgcga
     1561 tcctgccgcg cattatcggc caggggcgcg cgtcggacct cctctacacc gggcggatgc
     1621 tcggcgccga agagggcgag cggtggggct tccacaaccg gctggtcgaa cccgacgcgc
     1681 tgctcgacga ggcaaccgcg ctcgcccgct cgctcgccac ggggccgact ttcgcgcacg
     1741 gcatcaccaa gacgcagctc ggcatcgaat gggcggtctc ggtcgaaacc gcgatcgaga
     1801 tggaagcgca ggcgcaggcg atctgcatgg cgaccaatga tttccgccgt gccttcgagg
     1861 cgtttgcgga caaacgcact cccgagttcg cgggcgactg atggccgacc gcagcttcct
     1921 cgactggccg ttcctcgacg atggccaccg cgcgctggcg caggacctcg acgcctggtg
     1981 cgcggcgcat ctgaccgacc atcattcgga cgatatcgac ggcgagtgcc gcgcgctcgt
     2041 ccgcgcgctc ggcgacgccg gctggctgcg ctattgcatc cccgcagctt atggcggggt
     2101 acatgacagc ctcgatatcc gttcgctcgc gctgatccgc gaaacgctcg cgcgccattc
     2161 ggggctcgcc gacttcgctt tcgcgatgca ggggctggga tcgggcacga tcagcctctt
     2221 cggcaccgag gcgcagaaac aggcttatct gcccgatgta acggctgggc ggaagatcgc
     2281 ggcgttcgcg ctcaccgagc ccgcgtcggg atcggacgtc gcgtcgatgg aaacgacggc
     2341 gacgcgcacc gccgacggct atgtcgtcac cggcgcgaag acctatatct cgaacggcgg
     2401 catcgccgac tattatgtcc tgttcgcccg caccggcgag gcgccgggcg cgaagggcgt
     2461 gtcggccttc atcgtcgacg ccgacacgcc agggctggac gtgaccgacc gcatcgcggt
     2521 gatcgcaccg cacccgctcg cgacgctgac cttcaccgat atggcgctcg ccgacaccgc
     2581 tttgctcggc gaggcggggc gcggcttcgc ccaggcgatg gcgacgctcg acatcttccg
     2641 caccaccgtc ggcgccgccg cgctc
//
LOCUS       DATMSU010000555         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_113663,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000555 DATMSU010000000
VERSION     DATMSU010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113663"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VN034_14840"
     CDS             <1..1989
                     /locus_tag="VN034_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61938.1"
                     /translation="AFDSAALDALQVRDLRSLSTAAPNVALEDVGTIPGTANFTIRGF
                     GIVSSIPSVEPSVGLFVDGVYLGMSAGAVIDVFDLESVEVLRGPQGLLFGRNTTGGAV
                     TLRTRRPGDRFQVRGRLAVETGPQYTAGLSVEGPVADNLRAKIALYYSNDQGWFHNDF
                     NDRPFGKRETMFVRPTVVWDISPDVETTLIYEHGRSRGDGTVAQNPAFQSGFQIDIDN
                     PGYFDIDWDALTSETNVNVALGDGVITNVAGYRRLNQNGSVDVDGLPALGFHSFVNLR
                     QHQFSEELRYAGTFGPLKATLGLYYFSQNYFYIERRVLSGGAVDRAFGGRIDQDSWGA
                     FSQLSYDVGAGFSLVGGLRYSWERKKADVATFRAPGLCDLDARTCDFDFPGEFPGATG
                     VKSWGSLTPKLGFQYEPGRDLLLYGSWSRGIRSGGFNVKSTAATIPPGPYDMETQDAF
                     ELGWKSTFLDRRVRFNAAIFQSTLRDLQRDINIPDPVSGVVQVTSNVGTARVRGVEGE
                     LTFAPFRGLTLGGSFGHLNGKYTKLAVDLNGAAPGLGYELDLTRLPKWSFGANATIVQ
                     PVGDGAELTLRADYGYRSRVAFTDNNSAYLNQVNDLSASARLSFLDDRFSVGVFGKNL
                     LNRVTQGGNTPLPPSVGGGFFVPINEGRTVGMDINLKI"
     gene            2002..>2665
                     /locus_tag="VN034_14845"
     CDS             2002..>2665
                     /locus_tag="VN034_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWV61939.1"
                     /translation="MTIKAEIIGVGMARHGYRADSGWRDLVFEAGSGALADAGIERSS
                     IDGGLVSVTCPEAFEQQNLGPLTAEELDLPSGPFAQVVGACAGGTMAFMQGVNMIRSG
                     QSECVIVVGVEKMSDVYSVSDALLSYPDQDYEAPAGFDYVDTMALMHARYMQKYGVAP
                     EHVAAFAMQDRWYASRNPNAIDYRRGALGIDEIMQSGWASRPITRAECARGCDGAAAV
                     ILA"
BASE COUNT          461 a          832 c          876 g          496 t
ORIGIN      
        1 gctttcgaca gcgcggcgct ggatgcgctt caggttcgcg acttgcgctc gctcagcacc
       61 gcggcaccca atgtggcgct ggaggatgtc ggcacgatcc cgggtacggc gaatttcacc
      121 attcgcggtt tcgggatcgt cagctcgata ccgtcggtcg aaccttcggt cggacttttc
      181 gtcgatggcg tttatcttgg catgtcggcg ggtgcggtga tcgacgtttt cgatctcgag
      241 agcgtcgagg tgctgcgagg ccctcaaggg cttttgttcg gccgcaatac aaccggcggc
      301 gccgtcacgc tgcgcacgcg gcgtccgggc gacaggtttc aggtgcgcgg ccggctggcc
      361 gtcgaaacgg ggccgcaata tacggccggc ctgagcgtcg aaggacccgt cgcagacaat
      421 ctccgcgcca aaatcgcgct ctattacagc aatgaccagg gctggtttca caacgacttc
      481 aacgaccggc cgtttgggaa gcgcgagaca atgttcgtgc gtccgaccgt cgtgtgggac
      541 atctcgcccg acgtcgagac gaccctgatt tacgagcatg gacgcagcag gggcgacggg
      601 acggtggcgc agaaccctgc cttccagtcg ggattccaga tcgatatcga taacccgggc
      661 tatttcgaca tcgactggga cgcgctgacg tcggagacca acgtcaacgt ggcgctcggg
      721 gacggggtca ttacgaacgt cgccggctac cggcggctga accagaacgg atcggtcgat
      781 gtcgacggct tgcccgctct gggttttcac tccttcgtca acttgcggca gcatcagttc
      841 agcgaagaac tccgctatgc ggggacgttc ggcccgctca aggcaaccct cgggctctat
      901 tatttcagcc agaactattt ctatatcgag cggcgcgtgc tgtccggcgg cgccgtcgat
      961 cgcgcctttg gcggccggat cgaccaggat agctggggcg ccttttcaca gctcagctac
     1021 gacgtcgggg cgggcttcag ccttgtcggc ggcctgcgct actcgtggga gcgaaagaag
     1081 gccgacgtcg cgactttccg ggcaccgggc ctgtgcgacc tcgatgcgcg gacgtgcgat
     1141 ttcgattttc cgggcgagtt tcccggggcg accggcgtaa aaagctgggg cagcctgacg
     1201 ccgaagctgg gctttcagta tgaaccgggc cgcgatttgc tgctctacgg aagctggagc
     1261 cgcggcattc gcagcggtgg attcaacgtc aagagtacgg ccgcgacgat tccgcccggc
     1321 ccctatgata tggaaacgca ggacgcgttc gagctcggct ggaaatcgac cttcctcgac
     1381 cggcgcgtcc gtttcaacgc ggcgatattc cagagcacgc tgcgcgatct gcagcgcgac
     1441 atcaacattc ccgatccggt gtcgggcgtg gtgcaggtga cgtcgaatgt cgggacggcg
     1501 cgggtgcgcg gcgtcgaagg cgagctgacg ttcgccccgt tccgcggtct gacgctgggc
     1561 ggcagcttcg gccacctgaa cggcaaatat acgaaactgg cggtcgacct gaacggggcc
     1621 gctccggggc tgggctacga actcgacctg acacggcttc cgaaatggag cttcggcgcg
     1681 aatgcgacga tcgttcagcc cgtgggggat ggtgccgaac tgacgctccg tgccgattac
     1741 ggatatcgca gccgcgtcgc cttcaccgac aacaactccg cctatctcaa ccaggtcaac
     1801 gatctgtcgg cgagcgcgcg gctttcgttt ctcgatgacc ggttctcggt cggggttttc
     1861 ggcaagaacc tcctcaaccg tgtgacgcag ggcggcaaca ccccgcttcc cccctcggtc
     1921 ggtggcggct tcttcgtgcc gatcaacgag gggcgcaccg tcggcatgga tatcaatctc
     1981 aaaatttagg gcgtccggac aatgaccata aaagcggaaa tcatcggcgt gggcatggcc
     2041 aggcatggct accgggccga cagcggatgg cgcgatctcg tgttcgaagc gggaagcggt
     2101 gccctcgccg acgccggcat cgaacgatcg tcgatcgacg gcgggctggt gtcggtcacc
     2161 tgcccggagg ccttcgaaca gcagaatctc ggcccgctca cggccgaaga gcttgacctg
     2221 cccagcggcc ccttcgcgca ggtcgtcggc gcgtgcgcgg gcgggaccat ggcgttcatg
     2281 cagggcgtca acatgatccg ttcgggccag agcgagtgcg tcatcgtcgt cggcgtcgag
     2341 aaaatgtcgg atgtctattc ggtgtcggac gcgctgctgt cctatcccga ccaggattat
     2401 gaagcgcccg cgggcttcga ctacgtcgat acgatggcgc tcatgcacgc gcgctacatg
     2461 cagaaatacg gcgtggcgcc cgaacatgtc gccgcctttg cgatgcagga ccgctggtat
     2521 gcctcccgca acccgaatgc gatcgactat cgccgcggcg cgctcggcat cgacgagatc
     2581 atgcagtcgg gctgggcgtc gcggccgatc acccgcgccg aatgcgcacg cggctgcgac
     2641 ggcgccgccg cggtcatcct cgccc
//
LOCUS       DATMSU010000556         4698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_151279,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000556 DATMSU010000000
VERSION     DATMSU010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151279"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VN034_14850"
     CDS             <1..234
                     /locus_tag="VN034_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWV61940.1"
                     /translation="LLAGDPASAPAAVRTLSGWQVPALPLKGGDIVARGIAAGPEVAR
                     ILKAVEAEWVAEDFPGAARVAELVDQKIGRTSD"
     gene            complement(207..1856)
                     /locus_tag="VN034_14855"
     CDS             complement(207..1856)
                     /locus_tag="VN034_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWV61941.1"
                     /translation="MLFVGWIDALPVPAALIRPMARGNFRLHASNAAFDRLSLSPVGV
                     DAPIELLRAIERASQYPEESQEFSCQLGEGPAARDLRGSIGPLPTESGDDGLFLLTLI
                     DRTQEMMTERNLRRELVSDSLTGLPNRAGFEELVEQRARTEGPGGDHAILLLDLARFS
                     RINEHIGPMAGDELIITVARRLKSSLRSGDILARTGGDEFAISSRIAGGRADVREMAR
                     RIRGCFDHPFRIGELKVSVDCALGCAIQPSIDTDIADQIRHAQIALKRAKQTDRIEIY
                     EPEAAMLSDNRFGLETELRNAIEEDRLHLAFQPLIELSSGRVAGFEALARWDNSSGTA
                     VSPTEFIPIAEDSGLIVPLGQWAIGKAAAVLAEWDKQNGGAVVDAYFSVNVSAIQLVR
                     DDVAGVVRQALEKHKIGGERLMIELTESAIIGDPDLALSVLSELKALDARVAMDDFGT
                     GYSNLAYLQRLPIDVLKIDRSFVEHMVDDRDKVAIVRTIQSLAEVLGMKTTAEGVETT
                     DQARLLSALGCDFGQGFLFARPMDSKAALDYWRQSLVRPIF"
     gene            complement(1979..4291)
                     /gene="parC"
                     /locus_tag="VN034_14860"
     CDS             complement(1979..4291)
                     /gene="parC"
                     /locus_tag="VN034_14860"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HWV61942.1"
                     /translation="MASTTDDLPPSDPPNPPGDSIDTPFDSALSERYLVYALSTITAR
                     SLPDLRDGLKPVHRRLLWAMRAMRLDPSQGYKKSARVVGDVIGKFHPHGDTAVYDAMV
                     RLAQDFSLRYPLVDGQGNFGNIDGDNAAAYRYTEARLTRVAIDLMQGLDEGTVDFRPT
                     YNGEDEEPEIMPGLFPNLLANGASGIAVGMATNIPPHNAAEVIGAALLLIENPGAELF
                     ELLEHVKGPDFPTGGIVVDSAETIAHAYETGRGGFRVRARFSTGKGADGVWEADGIEK
                     LSGGTWQLVISEIPYGVAKGKLIEQIAQLIADKKLPILEDVRDESAEDLRIVLEPKSR
                     NVDPEVLKDSLFRLTDLESRFALNLNVLDATRTPKVMGLKQLLTEWLQHQIIVLVRRA
                     QHRINKIDDRLELVAGYIIAFLNLDRIIEIIRTEDEPKPVMIEEFILTDRQAEAILNM
                     RLRSLRKLEEMELKREQADLTAEREDLAKLVESPARQKKRLRKDLEKLRDVYGLETLL
                     GARRTTIAEAAPTREIPLDAMIEKEPVTVILSKRGWIRAQRGHVAADQWSEFKFKEGD
                     ELLFAAHAQTTDKLLIAASDGRFFTVGADKLPGGRGFGEPLRLMVDIDPEARIVAMIP
                     ASADGRLLLASTSGHGFIAQMSEVVAETRKGRNVVNLKPKAALAVVRSIAPTDDSVAA
                     VGENRKLVVFPLAEVPVMARGQGVMLQRYRDGGLSDAVSFAFAEGLSWAMGGDTGRTR
                     TETDLAPWRVARGAAGRMPPTGFPRNNRFG"
     gene            complement(4487..>4698)
                     /locus_tag="VN034_14865"
     CDS             complement(4487..>4698)
                     /locus_tag="VN034_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HWV61943.1"
                     /translation="QSLEALTAAVAPAVAAATVAAPTVAGRTMEDVVLDALRPMLKDW
                     LDANLPPLVEAMVAKEISRITGRKF"
BASE COUNT          755 a         1526 c         1575 g          842 t
ORIGIN      
        1 cttctcgcgg gcgatccggc gtcggcgccc gctgccgtcc gaaccctgtc gggctggcag
       61 gttcccgcgc tgccgctgaa gggcggcgac atcgtggcgc gcggaattgc cgcggggccc
      121 gaggtcgcgc gcatcctgaa agcggtcgag gccgaatggg tggcggaaga ttttcccggc
      181 gcggcgcgcg tcgccgagct cgtcgatcag aagatcggcc gcaccagcga ctgacgccaa
      241 tagtcgagcg ccgccttgct atccattggc cgcgcgaaca ggaaaccctg cccgaaatcg
      301 cagccgagcg ccgacagcag ccgtgcctgg tcggtcgtct cgaccccttc ggcggtcgtc
      361 ttcatcccca gcacttcggc caggctctgg atcgtgcgga cgatcgcgac cttgtcgcgg
      421 tcgtcgacca tatgctcgac gaagctgcgg tcgatcttga gcacgtcgat cggcaggcgc
      481 tggagatagg cgaggttcga atagccggtg ccgaagtcat ccatcgcgac gcgcgcgtcg
      541 agcgccttca attcgctcag caccgacagc gcgaggtcgg gatcgccgat gatcgcgctt
      601 tcggtgagtt cgatcatcag cctttcgccg ccgattttgt gcttttcgag cgcctggcgg
      661 acgaccccgg cgacatcgtc gcggaccagc tggatcgccg agacattgac cgagaaatag
      721 gcgtcgacca ccgcgccgcc attctgcttg tcccattcgg cgagcaccgc cgccgccttg
      781 ccgatcgccc attggccgag cgggacgatc agccccgaat cttcggcgat cgggatgaat
      841 tcggtcggcg agaccgcggt gccgctgctg ttgtcccagc gcgcgagcgc ctcgaaaccg
      901 gcgacgcggc cgctcgacag ttcgatcagc ggctgaaagg cgaggtggag ccggtcttcc
      961 tcgatcgcgt tgcgcagctc ggtctcgagt ccgaagcgat tgtccgacag catcgcggct
     1021 tcgggttcat agatttcgat gcgatcggtc tgcttcgcgc gcttgagcgc gatctgcgca
     1081 tggcggatct gatcggcgat atcggtgtca atcgacggct ggatcgcgca gcccaatgcg
     1141 cagtcgacgc tgaccttgag ttcgccgatg cggaacggat gatcgaagca gccgcggatg
     1201 cgccgcgcca tttcgcgcac atcggcgcgg ccgcccgcga tgcgcgacga gatggcgaat
     1261 tcgtcgccgc cggtgcgcgc caaaatgtcg ccgctgcgca ggctcgactt caatcgccgc
     1321 gccacggtga tgatcagctc gtcgcccgcc atcgggccga tatgttcgtt gatgcgcgaa
     1381 aagcgcgcga ggtcgagcag caggatcgca tggtcgccgc ccggcccctc ggtacgtgcg
     1441 cgctgttcga ccagttcctc gaatccggcg cggttgggga gcccggtcag gctgtcggag
     1501 acgagctcgc ggcgcaggtt gcgctcggtc atcatctcct gcgtccggtc gatcagcgtc
     1561 agcaggaaca gcccgtcgtc gcccgattcg gtgggcagcg ggccgatcga tccgcgtagg
     1621 tcgcgcgccg ccggtccttc gccgagctgg cacgagaatt cctgcgattc ctcgggatat
     1681 tgcgacgcgc gctcgatcgc gcggagcagt tcgatcggcg catcgacgcc aaccggcgac
     1741 aggctgagcc ggtcaaaggc ggcgttgctc gcatgaagcc ggaaattgcc gcgcgccatc
     1801 ggccggatca gcgcggccgg taccgggaga gcgtcgatcc atccgacgaa caacaagggc
     1861 cggtcttcgc cggttctatc aataggcata acagagggtt ttgtgcgacc tttccccatt
     1921 gcccaatgcc taggtcacgg gaagtaaaga agggatttac ggctacatat aaatacggtc
     1981 agccgaagcg attattccgc gggaagcccg tcggcggcat ccggcccgcc gcgccgcgcg
     2041 cgacgcgcca gggcgcgagg tcggtttcgg tccgcgtccg gccggtgtcg ccgcccatcg
     2101 cccagctcaa accctcggca aaggcgaagc tgaccgcgtc ggagaggcca ccgtcgcgat
     2161 agcgttgcag catgacaccc tggccgcgcg ccatcaccgg cacctcggcg agcgggaaga
     2221 cgacgagctt gcgattctcg ccgaccgccg cgacgctgtc gtcggtagga gcgatcgagc
     2281 ggacgacggc gagcgccgcc ttcggcttca gattgacgac gttgcgcccc ttgcgcgtct
     2341 cggcgaccac ttcgctcatc tgcgcgatga aaccgtggcc gctcgtcgag gcgagcagca
     2401 gccgcccgtc ggcgctcgcc gggatcatcg ctacgattcg cgcctcggga tcgatgtcga
     2461 ccatcaggcg cagcggctcg ccgaaaccgc gaccgccggg caatttgtcg gcgccgacgg
     2521 tgaagaaacg cccgtcgctc gcggcgatca gcagcttgtc ggtggtttgc gcgtgcgccg
     2581 cgaacagcag ctcgtcgcct tccttgaact tgaactcgct ccactggtcc gccgcgacat
     2641 ggccgcgctg ggcgcgaatc cagccgcgct tcgacaggat caccgtcacc ggctcctttt
     2701 cgatcatcgc gtcgagcggg atttcgcgcg tcggggcggc ttcggcgatc gtcgtgcggc
     2761 gcgcaccgag caaagtctcg agcccataga cgtcgcgcag cttttcgagg tccttgcgca
     2821 gccgcttctt ctgccgcgcc gggctctcga caagcttcgc gagatcctcg cgttcggcgg
     2881 tcaaatcggc ctgctcgcgc ttcagctcca tttcctcgag cttgcgcaag ctgcgcaggc
     2941 gcatgttgag gatcgcctcg gcctgccggt cggtcaggat gaactcttcg atcatcaccg
     3001 gcttgggctc atcctcggtc cgaatgatct cgatgatccg gtcgaggttg agaaacgcga
     3061 tgatatagcc cgcgacgagt tcgagccggt cgtcgatctt gttgatgcgg tgctgcgcgc
     3121 ggcggacgag cacgatgatc tggtgctgca gccattcggt gagcagctgt ttcagcccca
     3181 ttaccttggg ggtgcgcgtc gcgtcgagca cgttcagatt gagcgcgaag cggctttcga
     3241 ggtcggtcag ccggaacagg ctgtccttca gcacctcggg atcgacgttg cggctcttgg
     3301 gttcgagcac gatgcgcaga tcctcggcgc tttcgtcgcg gacatcctcg aggatcggca
     3361 atttcttgtc ggcgatcaac tgcgcgatct gttcgatcag cttgcccttg gcgacgccat
     3421 aggggatttc gctgatcacg agctgccagg tgccgcccga caatttctcg atgccatcgg
     3481 cttcccagac accgtccgcg cccttgccgg tcgaaaagcg cgcgcggacg cggaagccgc
     3541 cgcggccggt ttcataggcg tgcgcgatcg tctccgcgct gtcgacgacg atgccgccgg
     3601 tcggaaagtc ggggcccttc acatgctcga gcagctcgaa cagttcggcg cccggattct
     3661 cgatcagcag cagcgcggca ccgatcactt cggcggcgtt gtgcggcggg atgttcgtcg
     3721 ccatgccgac cgcgatcccg ctcgcgccat tggcgagcag gttcgggaag agccccggca
     3781 tgatttcggg ctcttcgtcc tcgccattat aggtcgggcg gaaatcgacg gtgccctcgt
     3841 cgagcccctg catcaggtcg atcgcgacgc gcgtcagccg cgcttcggta tagcgatagg
     3901 cggcggcgtt atcgccgtcg atattgccga aattgccctg accgtcgacg agcggatagc
     3961 ggagcgagaa atcctgcgcg aggcggacca tcgcgtcata gaccgcggtg tcgccgtgcg
     4021 ggtggaattt gccgatcacg tcgccgacga cgcgcgccga cttcttgtaa ccctggctcg
     4081 gatcgagccg catcgcgcgc atcgcccaga gcaggcggcg gtgaacgggc ttcagcccgt
     4141 cgcgaagatc gggcagcgag cgcgcggtga tcgtcgacag cgcatagacg agatagcgtt
     4201 cggacagcgc gctgtcgaac ggggtatcaa tgctgtcgcc cggagggttt gggggatctg
     4261 aaggaggaag gtcgtctgtc gtactggcca ttgtccgcag ccgatagcaa ggctggcgcg
     4321 ccaaggcaaa gccttgcgtc gacgcggcgc gatacggggg cagctaagcg gttttccgcg
     4381 ctccgtcctt cggggttccc gtgcgaggga agggcgacag caacccgccg aaagccgtca
     4441 atccggccat cgcatccgaa ggccgcacgc gttgggaaat tggcgtctag aatttgcggc
     4501 cggtgatccg gctgatttcc ttggccacca tcgcctcgac gagtgggggc aggttggcgt
     4561 cgagccagtc cttgagcatc gggcgcagcg cgtcgaggac gacatcctcc atcgtgcggc
     4621 cggcgacggt cggggcggca acggttgcag cggcaaccgc gggcgcgacc gcggcggtca
     4681 gcgcttcgag cgactggc
//
LOCUS       DATMSU010000557         5774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_84835, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000557 DATMSU010000000
VERSION     DATMSU010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84835"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      225..315
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      316..424
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            477..1601
                     /gene="gcvT"
                     /locus_tag="VN034_14870"
     CDS             477..1601
                     /gene="gcvT"
                     /locus_tag="VN034_14870"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWV61944.1"
                     /translation="MREDEEIPIETATLPLDAWHRAKGGRMVEFAGYWMPIQYEGIMA
                     EHLWTRENAGLFDVSHMGQLQLSGDGVAEALEALVPGDISALKPGRMRYSLLLDEDGG
                     ILDDLMITNEGDQFGIVVNGAVKWEDIGHLRENLPDHITLNHNEDYGLLALQGPKAVD
                     ALVRLVPEAATLVFMQATRATWQGHAIALSRSGYTGEDGFEISLPNDALTAFADALCA
                     MDEVKPIGLGARDSLRLEAGLPLYGHDLTPAIDPAEADLGFAVSKRRREEENFPGAAR
                     ILGHLEDGPPRKRVGLTVDGKLPVREGAKLFDGEDEIGVVTSGGFAPSVGAPIAMGYV
                     PTGLSEPGTAIAAEVRGKLVHCTVTAMPFIPHRYVRKQGA"
     gene            1605..1976
                     /gene="gcvH"
                     /locus_tag="VN034_14875"
     CDS             1605..1976
                     /gene="gcvH"
                     /locus_tag="VN034_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWV61945.1"
                     /translation="MPRYYTEEHEWIDVDGDVATVGITDFAQGQLGDIVFVEVPDTGA
                     ELTAGGDAAVVESVKAASDVYAPADGTVTEGNGQLEEDPALVNSDPEGEGWFFRMTLS
                     DKGQLDGLMDAKGYKAFCDAL"
     gene            2016..2822
                     /locus_tag="VN034_14880"
     CDS             2016..2822
                     /locus_tag="VN034_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61946.1"
                     /translation="MTDAAPANCRLMVATPIYDGAQGTYVRAALGLALAAQAEGVSVR
                     FEFILHEAQIHRARSLLADLFVQSDCTHLLFVDADIDFAAADIFAMIGAMAGRPDCAI
                     LGAAVPRRTVNWPQVARAAERGLGKDNAADLARYAGDFALSFLHADQRFALTDLVELS
                     QLGTGLMLIRRDVVETLCARHPELVFRTDARDRQGTGVGESVHALFLPEIDPTSGLML
                     SEDYAFCHRARDAGFRVWLAPWVRTTHSGPAIFRGSLADLAPLFASSSDS"
     gene            2835..4193
                     /gene="gcvPA"
                     /locus_tag="VN034_14885"
     CDS             2835..4193
                     /gene="gcvPA"
                     /locus_tag="VN034_14885"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWV61947.1"
                     /translation="MRYLPLTPDDRAAMLGAIGAASIDDLFADVPAEARLDGPIAGLP
                     NHASELAVERHMGALARRSRAAGEGPFFLGAGAYRHHVPASVDHLIQRGEFLTAYTPY
                     QPEIAQGTLQMLFEFQSQVARLLGTDVANASMYDGSTACWEAIVMARRITKRAKALLS
                     TGLHPHYRSVARTMAKYTGDVLVDGDPALEAGTDWAALANCIDKETSCVVVQYPDILG
                     RIDDMTKLAEACQAAGALLIAVVTEPVALGLIKSPGEMGADIVVGEGQSLGVGLQFGG
                     PYVGLFACKTKYVRQMPGRLCGETVDANGMRGFVLTLSTREQHIRREKATSNICTNSG
                     LCALAFSIHMTLLGEAGLRQLAAINHGRAKAAAAELAKVPGVSVLNDSFFNEFTLLLP
                     TAARPVIHKLAEKDILGGVSLGRLYPDNDGLEIGLVVAVTETVTEADIAAFAAALKEV
                     LA"
     gene            4226..5734
                     /gene="gcvPB"
                     /locus_tag="VN034_14890"
     CDS             4226..5734
                     /gene="gcvPB"
                     /locus_tag="VN034_14890"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWV61948.1"
                     /translation="MNVAGGADDNVTFTGNRALMLEEPLIFEIGGRETTGVDFDEEAP
                     VTVLGALARTAPIGLPGLSESETVRHYTRLSRQNYAIDLGLFPLGSCTMKHNPRLNEK
                     VARMPGFADVHPLQPQETVQGAYAVIHELAEWLITLTGMHCVAMSPKAGAHGELCGIL
                     CIKAALEARGEDRRVILVPESAHGTNPATAAFAGFTVEDIPATPEGRVDLSALKARLG
                     PDVAGVMITNPNTCGLFERDMKAISDAVHAAGGYVYCDGANFNAIVGRVRPGDLGVDA
                     MHINLHKTFSTPHGGGGPGSGPVVLSEALAPFAPLPFVTKQGDDFRLIEEENASEDHP
                     QTFGRMTAFHGQMGMFTRALTYILSHGADGLKQVAEDAVLNANYVLRSLEDVLDAPFA
                     ASGPCMHEALFSDKGLAEGFSTLDIAKGLIDEGYHPMTVYFPLVVHGAMLVEPTETES
                     KAVLDQFIGALRSIALRAKNGDPALKSAPHFAPRARLDETLAARRPVLAWEG"
BASE COUNT          987 a         1968 c         1880 g          939 t
ORIGIN      
        1 acgatcgccg cgccgctgac cgacgcgctg ctccacggcg ccttccgccg ctaccgctcg
       61 atttcgggcg ataaggtcgc cgccgcgatc gtcgcgctcg ccgggcgaga ggagccgggc
      121 gtgtatatcc acgaaaacga cgcgatccgc gcgctcgccg attgactccg cacaggcgcg
      181 gcgccaaggc tcgcggcgtc tgtcgggttc cgccaaggga ttcgaccggc cgcgcgggag
      241 agcgcggggg caaccccgcc accgaaggag caaccgcccc ggaaactctc aggtcaagcg
      301 gaccgcgcag gctggaacgg acactctgga aagcgttccg gctgtttctg ccggaaccac
      361 cgaaggggta actccatgat atcgtggagg aaaactctca ggttcccgtg acagaggggg
      421 catggcacag ggcggcaccg ggccgcgcct tgccgtgtca acgacgggag tgcgatatgc
      481 gcgaagatga agaaattccg atcgaaacgg ccaccctgcc gctcgacgcc tggcaccgcg
      541 cgaaaggcgg ccgcatggtc gaattcgccg gatactggat gccgattcag tatgaaggca
      601 tcatggccga acatctctgg acccgcgaaa acgcaggcct gttcgacgtc agccacatgg
      661 gacagctgca gctttcgggc gacggcgtcg ccgaagcgct cgaagccctc gtccccggcg
      721 acatttcggc gctcaaaccc ggccggatgc gctactcgct gctgctagac gaagacggcg
      781 gcatcctcga cgatctgatg atcaccaacg aaggcgacca gttcggcatc gtcgtcaacg
      841 gcgcggtgaa atgggaggat atcggccacc tgcgcgaaaa tctgcccgat catatcacgc
      901 tcaaccataa tgaggattat ggtctgctgg cgctgcaggg acccaaagcg gtcgatgcgc
      961 tcgtgcggct ggtgcccgaa gccgcaacgc tcgtcttcat gcaggcgacc cgcgcgacct
     1021 ggcaaggcca tgcgatcgcg ctcagccgct cgggatacac cggcgaggac ggcttcgaaa
     1081 tctcgctgcc gaacgacgcg ctcactgcct tcgccgacgc gctttgcgcg atggatgaag
     1141 tcaagccgat cggcctcggt gcgcgcgact cgttgcggct cgaagcgggc ctgccgctct
     1201 acggccatga cctcaccccc gcgatcgacc ccgccgaagc cgacctcggt ttcgcagtca
     1261 gcaagcgccg ccgcgaggaa gagaattttc ctggcgcggc gcgcatcctc ggccatctcg
     1321 aagacggccc gccccgcaag cgcgtcggcc tgaccgtcga cggcaagctg ccggtgcgcg
     1381 agggcgccaa gctgttcgac ggcgaggacg aaatcggcgt cgtgacgtcg ggcggctttg
     1441 cgcccagcgt cggtgcgccg atcgccatgg gctatgtccc gacaggcctg tccgagcccg
     1501 gcaccgcgat tgccgccgaa gtgcgcggca agctggtcca ttgcaccgtc accgccatgc
     1561 cattcattcc acaccgctat gtgcgcaaac agggagcctg atcgatgccg cgttattata
     1621 ccgaagaaca tgaatggatc gacgtcgacg gcgacgtcgc gacggtcggc atcaccgact
     1681 tcgcgcaggg ccagctcggc gacatcgtct tcgtcgaggt tcccgacacc ggcgccgaac
     1741 tgaccgcggg cggcgatgcc gcagtggtcg aatcggtgaa ggccgccagc gacgtctatg
     1801 cccccgccga cggcaccgtg accgaaggca atggacagct cgaggaagac cctgcgctcg
     1861 tcaattcgga ccccgaaggc gaaggctggt tctttcgcat gacgctgtcc gacaagggcc
     1921 agctcgacgg cctgatggac gcaaagggct acaaggcctt ctgcgacgcc ctgtaaccgt
     1981 tcgcccctcc cgcgggcggg agggggaggg atgcgatgac cgacgccgcg ccagccaatt
     2041 gccgcctgat ggtggcgacg ccgatctatg acggcgcgca gggcacctat gtccgcgcgg
     2101 cgctgggtct cgcgctggcg gcgcaggccg agggcgtctc ggtgcggttc gagttcatcc
     2161 tccacgaagc gcagatccac cgcgcgcgca gcctgcttgc cgatctgttc gtgcagagcg
     2221 attgcacgca tctgctcttc gtcgatgccg acatcgactt cgccgccgcc gatattttcg
     2281 cgatgatcgg ggcgatggcg ggccggccgg attgcgcgat cctgggggcg gcggtgccgc
     2341 gccgcacggt caactggccg caggtcgcgc gcgctgccga gcgcggattg ggaaaggaca
     2401 atgcggccga cctcgcgcgc tatgcgggag atttcgcgtt gagcttcctg cacgccgacc
     2461 aacgcttcgc gctcaccgat ctcgtcgagc tgtcgcagct cggcaccggg ttgatgctga
     2521 tccgccgcga cgtcgtcgaa acgctttgcg cgcggcaccc cgaactcgtc ttccggaccg
     2581 acgcgcggga ccggcaggga acgggggtgg gcgaaagcgt ccacgcgctc ttcctgcccg
     2641 agatcgatcc gaccagcggc ctgatgctgt cggaagacta tgccttctgc caccgcgcgc
     2701 gcgatgcggg ctttcgcgtc tggctcgccc cctgggtccg cacgacgcac agcggccccg
     2761 ccatttttcg cggatcgctc gccgatctcg caccgctttt tgcttcttct tccgatagct
     2821 gaccggagta tttcatgcgt tatctccctc tcactcccga cgatcgcgcg gcgatgcttg
     2881 gcgccattgg cgcggcgtcg atcgatgatc tgttcgccga cgtgcccgcc gaggcgcggc
     2941 tcgacggccc gatcgcgggc ttgccgaacc atgcgagcga gcttgcggtc gagcgccaca
     3001 tgggcgcgct cgcacgccgc agccgcgcgg cgggcgaagg acctttcttc ctcggcgccg
     3061 gggcctatcg ccaccatgtt ccggcgagcg tcgatcacct gatccagcgc ggcgaattcc
     3121 tcaccgccta tacgccctat cagcccgaaa tcgcgcaggg cacgctgcag atgctgttcg
     3181 aattccagtc gcaggtcgcg cggcttctcg gcaccgacgt cgccaatgcc tcgatgtacg
     3241 acggctcgac cgcctgctgg gaagcgatcg tcatggcgcg ccgcatcacc aagcgtgcca
     3301 aggctttgct ctcgaccggg ctccacccgc attaccgcag cgtcgcgcgc acgatggcca
     3361 aatataccgg cgatgtgctg gtcgacggcg acccggcgct cgaagcgggc accgactggg
     3421 ccgcactggc gaactgcatc gacaaggaga cgagctgcgt cgtcgtccaa tatcccgaca
     3481 tcctcggccg catcgacgac atgacgaagc tcgctgaggc gtgccaggcg gcgggcgcgc
     3541 tgctgatcgc ggtggtgacc gaacccgtcg cgctcggcct catcaagtcg ccgggcgaaa
     3601 tgggcgccga catcgtggtg ggcgaggggc agtcgctagg cgtcggcctt cagttcggcg
     3661 ggccttatgt gggcctcttc gcgtgcaaga ccaaatatgt ccgccagatg cccgggcgcc
     3721 tctgcggcga aaccgtcgat gccaacggca tgcgcggctt cgtgctgacg ctctcgacgc
     3781 gcgagcagca tattcgccgt gagaaagcga cgagcaatat ctgcaccaat tcaggtcttt
     3841 gcgcgctggc gttcagcatt cacatgaccc tgctcggcga agcggggctg cgccagcttg
     3901 cggcgatcaa ccacggccgg gcaaaggcgg ccgccgccga actcgcgaag gtgccgggcg
     3961 tgtcggtgct gaacgacagc ttcttcaacg aattcacctt actgctgccg accgcggcgc
     4021 ggccggtgat ccacaagctt gccgaaaagg acatcctcgg cggcgtctca ttgggccgcc
     4081 tctatcccga caacgacggt ctcgaaatcg gcctcgtcgt cgccgtgacc gaaaccgtga
     4141 ccgaggcgga tatcgccgcc ttcgccgcgg ccctgaagga ggtgctggca tgaccgcgct
     4201 caaccgcgcc ggctggcgcc ccgaaatgaa cgtcgccggc ggcgccgacg acaatgtcac
     4261 cttcaccggc aatcgcgcgc tgatgctcga agaaccgctg atcttcgaga tcggcggccg
     4321 tgagacgacc ggcgtcgatt tcgacgagga ggcgcccgtg accgttctcg gcgcactcgc
     4381 gcggaccgcg ccgatcggcc tgccggggct cagcgagtcc gaaacggtgc gccattacac
     4441 gcgcctgtcg cggcagaatt acgcgatcga cctcggcctc ttcccgctcg gcagctgcac
     4501 gatgaagcat aatccgcgcc tcaacgaaaa ggtcgcgcgg atgccgggct tcgccgacgt
     4561 ccacccgctc cagccgcagg aaacggtgca gggcgcctat gcggtgattc acgaactcgc
     4621 cgaatggctg atcacgctca cggggatgca ctgcgtcgcc atgtcgccca aggccggcgc
     4681 gcacggcgag ctttgcggca tcctctgcat caaggcggcg ctggaagcgc gcggcgagga
     4741 ccggcgcgtc attctcgttc ccgaaagtgc gcacggcacc aatccggcga cggcagcctt
     4801 tgcgggcttc acggtcgagg atatcccagc gacccctgag ggccgcgtcg acctctcggc
     4861 gctcaaggct cgcctcggtc cggatgttgc gggggtgatg atcaccaatc ccaacacctg
     4921 cgggctgttc gagcgcgaca tgaaagcgat ctcggacgcg gtccatgcgg cgggcggcta
     4981 tgtctattgt gacggcgcca acttcaacgc gatcgtcggc cgcgtgcgtc ccggcgacct
     5041 cggcgtcgat gcgatgcaca tcaacctgca caagaccttc tcgaccccgc atggcggcgg
     5101 cggacccggc tcgggccccg tcgtgctgtc cgaagctttg gctccgttcg caccgctgcc
     5161 tttcgtcacc aagcagggcg atgatttccg gctgatcgag gaggagaatg cgagcgagga
     5221 tcatccgcag accttcggcc gcatgaccgc cttccacggc cagatgggca tgttcacgcg
     5281 cgcgctgacc tatattctca gccacggcgc cgacgggctg aaacaggtcg ccgaggatgc
     5341 ggtgctcaac gccaattatg ttctgcgcag cctcgaggac gtcctcgacg cgcccttcgc
     5401 ggcgtcgggc ccatgcatgc acgaggcatt gttcagcgac aagggcctcg ccgaaggctt
     5461 ctcgacgctc gacatcgcca aggggctgat cgacgagggc tatcacccga tgacggtcta
     5521 tttcccgctc gtcgtccatg gcgcgatgct cgtcgagccg accgagaccg agagcaaggc
     5581 ggtgctcgac cagttcatcg gcgcgctgcg cagcatcgcg ttgcgcgcga agaatggcga
     5641 tcccgcgctc aaatcggcgc cgcacttcgc gccccgcgcg cggctcgacg aaacgctcgc
     5701 ggcgcggagg ccggtgctgg cgtgggaggg gtgatcggag cccatccaag cggccggcgc
     5761 ggatcaagct aatc
//
LOCUS       DATMSU010000558         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_18630, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000558 DATMSU010000000
VERSION     DATMSU010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18630"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="plsX"
                     /locus_tag="VN034_14895"
     CDS             <1..805
                     /gene="plsX"
                     /locus_tag="VN034_14895"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWV61949.1"
                     /translation="VAIRKKASSMSMAIAAVKAGEAGAAVSAGNTGALMAMAKLALRT
                     MPGIDRPALAALLPTLGDNDVVMLDLGANTDCDANNLIQFAVMGAAYARTAMGLERPR
                     VALLNIGTEELKGTGEIRDAAARLRGAKGLPMEFTGFIEGDKLSRGNVDVIVHDGFSG
                     NIALKTIEGTARFVTDLLRRAFTSSVRSKIGFLISRPATELLRHHLDPNNHNGAVFLG
                     LNGVVLKSHGSADAKGVANCVHLCAELIEKDITRQVTEDLANFRSGDAA"
     gene            802..1767
                     /locus_tag="VN034_14900"
     CDS             802..1767
                     /locus_tag="VN034_14900"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWV61950.1"
                     /translation="MTLRAILAGTGSALPRTRVSNEELARRVDTSDEWIVERTGIRFR
                     HIAEPDETTATLGADAAKQALAAAGLEPSDIGLIIVATATPDNTFPASATKVQALLGS
                     PDCIAFDVAAVCSGFLYAVSVADSMLRTGAAKHALVIGSETFSRILDWEDRTTCVLFG
                     DGAGAIVLSAEEVADDRGILATRLHAEGKYSDMLYVDGGPSTTGTVGHVRMQGREVFR
                     HAVTNLASVLGEVMEEIGLSAEEIDWVVPHQANKRIIDATAKKLGLPAERVVLTVDQH
                     ANTSAASVPLALDLAVRDGRIKRGDLVVLEAMGGGFTWGAAVLRV"
     gene            1956..2249
                     /locus_tag="VN034_14905"
     CDS             1956..2249
                     /locus_tag="VN034_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HWV61951.1"
                     /translation="MTAGTLTRADIASRINQQIGLSRNESAAIVESILDHMSDALAVG
                     QNVKISGFGTFVLRDKAQRIGRNPKTGIEVPILPRRVMTFRASQTMRARVAGK"
     gene            2279..>2579
                     /locus_tag="VN034_14910"
     CDS             2279..>2579
                     /locus_tag="VN034_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWV61952.1"
                     /translation="MAGDDGKAAGAMLAIGELAVRIGVPTHVLRYWETRFPQLKPLQR
                     SGRRRYYRAEDVALAERIHHLLHVRGFTVDGARKALSEAGSAPAPRPASMREMSDA"
BASE COUNT          430 a          855 c          862 g          432 t
ORIGIN      
        1 cgtcgccatt cgcaagaagg ccagctccat gagcatggcc atcgctgcgg tgaaggctgg
       61 cgaagccgga gcagccgtgt ctgcgggcaa taccggcgcg ctgatggcga tggcgaagct
      121 cgcgctgcgc acgatgccgg ggatcgaccg gccggcgctc gcggcgctgc tgccgacgct
      181 cggcgacaat gacgtcgtca tgctcgacct tggcgccaac accgattgcg acgccaataa
      241 tctgatccag ttcgcggtca tgggcgccgc ttatgcgcgc accgcgatgg ggctcgaacg
      301 cccgcgcgtc gcgctgctca acatcggcac cgaggaactg aagggcaccg gcgagatccg
      361 cgacgccgcg gcgcggcttc gcggcgcgaa ggggctgccg atggagttca ccggcttcat
      421 cgagggcgac aaattatcgc gcggcaatgt cgacgtgatc gtccatgacg gcttttcggg
      481 caatatcgcg ctgaagacga tcgaggggac ggcgcgcttc gtcaccgacc tcttgcgccg
      541 cgcctttacc tcttcggtgc gctcgaagat cggcttcctg atctcgcgcc ccgcgaccga
      601 gttgctgcgc caccatctcg accccaataa tcacaatggc gcggtgttcc tcggcctcaa
      661 cggcgtggtg ctcaagagcc atggcagcgc cgatgccaag ggagtcgcga actgcgttca
      721 cctctgcgcc gagctgatcg aaaaggacat tacgcgtcag gtgaccgagg atctcgccaa
      781 tttccgcagc ggcgacgcgg catgacgctg cgcgcgatcc tggccggcac gggttcggcg
      841 ctgccgcgca cccgcgtgtc gaacgaggaa ctggcgcggc gcgtcgacac cagcgacgaa
      901 tggatcgtcg agcgcaccgg catccgtttc cgccatatcg ccgagcccga cgagacgacc
      961 gcgacgctcg gcgccgatgc ggcgaagcag gcgctcgccg ccgcggggct cgaaccctcc
     1021 gacatcggcc tgatcatcgt cgcgaccgcg acccccgata acaccttccc ggcgagcgcg
     1081 accaaggtgc aggcgctgct cgggtcgccc gactgcatcg cctttgacgt cgccgcggtc
     1141 tgctcgggct tcctctatgc cgtctcggtc gccgattcga tgctgcgcac cggcgcggcg
     1201 aaacacgcgc tggtgatcgg cagcgagacc ttcagccgca tcctcgactg ggaggatcgc
     1261 acgacctgcg tgctgttcgg cgatggcgcg ggcgcgattg tgctgtcggc cgaagaggtc
     1321 gccgacgacc gcggcatcct cgcgacccgg ctccacgccg agggcaaata ttcggacatg
     1381 ctctatgtcg acggcgggcc gtcgacgacc ggcacggtcg gccatgtccg gatgcagggc
     1441 cgcgaagtgt tccgccacgc ggtcaccaac ctcgcgtcgg tgctgggcga agtgatggaa
     1501 gagatcggcc tgtcggcgga agagatcgat tgggtcgtcc cgcaccaggc gaacaagcgg
     1561 atcatcgacg cgaccgcgaa aaagctgggc ctgcccgccg agcgcgtcgt gctgacggtc
     1621 gaccagcatg ccaacacgtc ggccgcctcg gtgccgctcg cgctcgacct cgccgtacgc
     1681 gacgggcgca tcaagcgcgg tgacctggtg gtgctcgaag cgatgggcgg cggcttcacc
     1741 tggggcgcgg cggtgctgcg cgtctagctt gcgttgccgc accggcccgc tcctatcgca
     1801 agcccccgaa actcgtcgcg tcaacttctc gctccatccg tttcagtttg gcggattaat
     1861 tcggatttgt tacatatgcg taacgggact cgtgacggtg ggggctgtca gagaaccggc
     1921 tgcatatttt cgcttcagag ggggaaggga tcaacatgac cgcagggacg cttacgcgag
     1981 ccgatatcgc cagccgcatc aaccagcaga tcggcttgtc gcgcaatgaa tcggccgcca
     2041 tcgtcgaatc gattctcgac catatgtccg atgcgctcgc ggtcggacag aatgtgaaga
     2101 tttcgggctt cggcaccttt gtgctgcgcg acaaggcgca gcggatcggc cgcaatccca
     2161 agacggggat cgaggttccg atcctgccgc gccgcgtgat gacctttcgt gccagccaga
     2221 cgatgcgagc gcgcgttgcc ggcaaatagg tcccgcccta tcgatgccga ggtctgacat
     2281 ggccggcgat gacggcaagg cggcgggtgc catgctggcg atcggcgaac ttgcggtgcg
     2341 catcggcgtg ccgacgcatg tgctgcgata ttgggaaacg cgtttcccgc agctgaagcc
     2401 gctccagcgt tcgggccgcc gccgctatta ccgcgccgaa gatgtcgcgc tcgccgagcg
     2461 tatccaccat ctgctgcatg tgcggggctt taccgtggac ggcgcgcgca aggcgctgtc
     2521 cgaggcaggc tcggcgcccg cgccgcgccc ggcatcgatg cgcgagatgt ccgatgccg
//
LOCUS       DATMSU010000559         3504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_113716,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000559 DATMSU010000000
VERSION     DATMSU010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113716"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..672)
                     /locus_tag="VN034_14915"
     CDS             complement(61..672)
                     /locus_tag="VN034_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWV61953.1"
                     /translation="MAAALTATAINCSLSTGGRKSSTDAMLAVLAEHFAAHEVTVADP
                     IRIAAHNIKWGVTSDEGEGDDWPRIREQILASDILIFGTPIWMGQAPSVAKLVMERMD
                     AFLSETDDKGRMPSTSKVAVAAIVGNEDGAHNVSSQIFQALNDVGWTIPAVAACYWVG
                     EAMGSVDFKDLEHRPRKVTETAKMVAANAAHLARLLKEAPYPG"
     gene            complement(726..2255)
                     /locus_tag="VN034_14920"
     CDS             complement(726..2255)
                     /locus_tag="VN034_14920"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWV61954.1"
                     /translation="MAGYSQGTGSIAKGCLAMRMRFIIPLTLAPLLLASCGGGSDGRD
                     DDPPTANAPPAFTSLQTASVAENSSAAYQATASDPDGDALTFAIDGGADADLFSITTA
                     GALRFDAPPDYDLAGDADGDNVYAVQLRVSDGSANATLAVNITVTNSREGIAVARVGT
                     GFDQPLYVAPIPGDSRVYVVEKGGNVYRFDPANGSRSLVLDITDISTDGERGLLGLAA
                     YPDHGSSQRLFVVATATNGAVQVRRYTLGRPDSSTAYDTVLSIPHGANNNHNGGWIGY
                     GPDGHVYVAVGDGGGSGDPGNNAQNRNSRLGKILRFAVGAGGNSYSPAPGNPFLGGGG
                     DPWVYAFGLRNPFRASFAGSTLIIGDVGQGAIEEVDIVTTTQPGLNFGWPFREGTQSY
                     SGTAPGGLTDPVLEYGHGTGPRQGRTVTGGYVYRGPVASLQDHYVFADFISDNIWTVP
                     FSSLVPGQTLASSRFARRNEDFAPDAGTLNSIASFGEDSAGNLFLISLGGDIFMVRQG
                     T"
     gene            complement(2308..2823)
                     /locus_tag="VN034_14925"
     CDS             complement(2308..2823)
                     /locus_tag="VN034_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61955.1"
                     /translation="MRIMILAAATLLATGCGKMAELASQTRTVAAPREEVFAKMFGDD
                     GAFAGLPLVTNGGSTRLYELVAEKGEPGFEKLIPDKRPEAYKVKIAVAMEIPREAHLI
                     YSVDDGALTTGLKFTFDELAPDRTRVAFTIDELAGHDAKGLAVNRVVLHKIARDALEK
                     LDDFDEVEEAA"
     gene            2969..3181
                     /locus_tag="VN034_14930"
     CDS             2969..3181
                     /locus_tag="VN034_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWV61956.1"
                     /translation="MQNRVREYREGAGWSQGELARRLGVSRQTINAVETDKYDPSLPL
                     ALRMARLFGLEVRELFIDHWIPEDEE"
     gene            3178..>3504
                     /locus_tag="VN034_14935"
     CDS             3178..>3504
                     /locus_tag="VN034_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61957.1"
                     /translation="MSHSTIDGRTRRWKKTAFGALTGAAFGAGSFSLLMWLAGEGLLV
                     AMQPSRVALAGVGLVYLLITVMIGFGLAAPRAGARLLNVGDAEELVDDRPKLLRSALY
                     MAWAGVT"
BASE COUNT          575 a         1105 c         1201 g          623 t
ORIGIN      
        1 cccgccttcg cgggggcgac gggatgattt gtgcctcgct tgcgacgcgg gcgcaagcgc
       61 tcagcccgga tagggtgctt ccttgagcaa ccgcgccaga tgcgccgcgt tggcggcaac
      121 catcttcgcg gtttcggtga ccttgcgcgg gcggtgctca agatccttga aatccaccga
      181 acccatcgct tcgccgaccc aatagcaggc ggcgaccgcg gggatcgtcc agccgacgtc
      241 gttcaaggcc tggaagatct gcgacgagac attgtgcgcg ccatcctcat tgccgacgat
      301 cgcggcgacc gcgaccttcg aagtgctcgg catccggccc ttgtcgtcgg tctcggagag
      361 gaaggcgtcc atccgctcca tcacgagctt ggcgacgctc ggcgcctgtc ccatccagat
      421 cggggtgcca aagatcaata tgtcggaagc gaggatctgt tcgcggatcc gcggccagtc
      481 gtcgccttcg ccctcgtccg atgtgacccc ccatttgatg ttgtgcgcgg cgatgcggat
      541 cggatcggcg accgtcacct catgcgcggc gaaatgttcg gcaagcacgg cgagcatcgc
      601 gtcggtcgag cttttccgtc ccccggtcga gagcgagcaa ttgatggcgg tggcggtgag
      661 ggcggcggcc atgatggctc ctttgcggat tatgtccccc gcttgcgaaa gccgcggcgg
      721 cgcgctcagg ttccctggcg caccatgaag atgtcgccgc cgaggctgat gaggaacagg
      781 ttgcccgcgc tgtcctcgcc gaaggaggcg atcgagttca gcgtccccgc gtcgggcgcg
      841 aaatcctcgt tccgccgcgc gaaacgcgaa gatgcgagcg tctgtccggg gacgaggctg
      901 gagaagggaa ccgtccagat attgtccgaa atgaagtcgg cgaagacata atgatcctgc
      961 agcgacgcga ccggcccgcg atagacatag ccgccggtga ccgtccgtcc ctgccgcgga
     1021 ccggtgccat ggccatattc gagcacgggg tcggtcaacc cgccgggcgc ggtgcccgaa
     1081 tagctttggg tgccctcgcg aaacggccag ccgaaattga gccccggctg cgtcgtcgtc
     1141 acgatatcga cctcttcgat cgcgccttgc ccgacgtcgc cgatgatcag cgtcgaaccc
     1201 gcgaaggagg cgcggaaggg gttgcgcagg ccaaaggcat agacccaggg atcgccgccg
     1261 cccccgagga aggggttgcc gggcgcgggc gaatagctgt tcccgcccgc gccgaccgcg
     1321 aagcgcaaaa tcttgccgag ccgcgaattc cggttctgcg cattgttgcc gggatcgccc
     1381 gatccgccgc cgtcgccgac cgcgacatag acatggccgt cgggaccata gccgatccag
     1441 ccgccattgt gattgttgtt cgcgccatgc ggaatgctga gcaccgtatc ataggcggtc
     1501 gaggaatcgg gccgtcccag cgtatagcgc cgcacctgca ccgcgccgtt cgtcgcggtc
     1561 gcgacgacga acagccgctg cgacgagcca tggtcgggat aggcggcgag cccgagcagc
     1621 ccgcgttcgc cgtcggtcga aatgtcggtg atgtcgagca cgaggctgcg gctgccgttc
     1681 gcggggtcga agcgatagac gttgccgccc ttttcgacga catagacgcg gctgtcgccg
     1741 gggatcggcg cgacatagag cggctgatcg aaccccgtgc cgacgcgcgc gaccgcgatg
     1801 ccctcgcggc tgttggtaac ggtgatattg accgccagcg tcgcgttcgc gctgccatcg
     1861 ctgacgcgta gctggacggc atagacattg tcgccgtcgg cgtcgccggc gaggtcgtaa
     1921 tcgggcgggg cgtcgaaccg cagcgcgccc gccgtggtga tcgaaaacag gtcggcgtcg
     1981 gcgccgccat cgatcgcgaa ggtcagcgcg tcgccgtcgg gatcgctcgc ggtcgcctga
     2041 taggcggcgg agctgttttc ggcgacgctg gcggtctgga gcgatgtgaa ggcgggcggg
     2101 gcgttggcgg tcggcgggtc gtcgtctctt ccgtcgcttc ctccgccgca cgacgcaagg
     2161 agcagggggg cgagggtgag gggaatgatg aagcgcatcc gcatggccag acagcctttc
     2221 gcgatcgaac cggtcccttg gctataacct gccatccgcc gaaaggctcc ctagccccgc
     2281 cgatttgccg caaatagatg atgccggtca ggcggcctct tccacctcgt cgaaatcgtc
     2341 gagcttttcg agcgcgtcgc gtgcgatctt gtggaggacg acgcggttga cggcgaggcc
     2401 cttggcatcg tgccccgcca gctcgtcgat cgtgaacgcg acgcgggtgc gatcgggtgc
     2461 cagttcgtcg aaggtgaatt tcagcccggt cgtgagcgcg ccgtcgtcga cgctgtagat
     2521 cagatgcgcc tcgcgcggga tttccatcgc gacggcgatc ttgaccttat aggcttcggg
     2581 ccgtttgtcg gggatcagct tctcgaaccc gggctcgccc ttttcggcga cgagttcgta
     2641 gagccgcgtc gatccgccgt tggtgacgag tggcaggccc gcgaaagcgc cgtcgtcgcc
     2701 gaacattttg gcgaacacct cttcgcgcgg cgccgcgacg gtgcgcgtct ggctcgcgag
     2761 ttcggccatc ttgccgcaac cggtcgcaag caaggtcgcg gccgccagga tcatgatacg
     2821 catcatcgaa catccccccg atcccgccgg cttttagccg cgaccgctta ccaaagcgta
     2881 atcgcggtgc gatgaccgat gtgtaaagag tgtttgacac cccaatgcga ggcctgtaaa
     2941 gaatgtttga caggctaagg aatgtgcgtt gcaaaatcgg gtgcgcgaat atcgcgaagg
     3001 cgcgggatgg agtcagggcg aactggcccg ccggctcggc gtgtcgcgtc agacgatcaa
     3061 cgcggtcgaa accgacaaat atgaccccag cctgccgctc gcgctgcgca tggcccgcct
     3121 gttcgggctc gaggtgcgcg agctgtttat cgatcactgg attccggagg atgaagaatg
     3181 agccattcga cgatcgacgg ccggacacgg cggtggaaaa agacggcatt cggcgcgctc
     3241 accggcgcgg cgttcggcgc gggcagcttt tcgctgctga tgtggctcgc gggcgaggga
     3301 ttgctcgtcg ccatgcagcc ctcgcgcgtc gcgttggcgg gtgtggggct ggtctatctg
     3361 ctgatcaccg tgatgatcgg tttcggcctc gccgcgccgc gcgccggggc gcggctgctc
     3421 aacgtcggcg atgccgagga actagtcgac gaccggccca agctgctgcg ttcggcgctc
     3481 tatatggcat gggcgggcgt cacg
//
LOCUS       DATMSU010000560         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_18659, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000560 DATMSU010000000
VERSION     DATMSU010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18659"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="wrbA"
                     /locus_tag="VN034_14940"
     CDS             <1..586
                     /gene="wrbA"
                     /locus_tag="VN034_14940"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HWV61958.1"
                     /translation="LYYSSYAHIEQMADAVAEGARAAGAEVDIRRVAETAPEAVVKAA
                     GFKTDTAHPLLSDPNDLVKYDAVVVGTPTRFGRMASQMASFWDTAGGVWAQGKLNGKV
                     GAAFTSTASQHGGQETTLFSVLTNLLHFGMTIVGLDYGYAGQMGVDEVKGGAPYGATT
                     IADGDGSRQPSEADLGGARYLGERVAKTAAKLAA"
     gene            591..1397
                     /locus_tag="VN034_14945"
     CDS             591..1397
                     /locus_tag="VN034_14945"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HWV61959.1"
                     /translation="MRRLPSLFLSHGSPMMALEPSPARDFLAGLGVRLPRPRAILFVS
                     AHHDAAYQGGRATVTASPAPPTIHDFGGFPDELFAMRYPAPGDPALADRVVELLAGHG
                     LDIAADPERGLDHGAWVPLSLIYPLADIPVVQLSIASNASPEWHHALGRALAPLRDEG
                     VLIAGSGSITHNLRALFSARLPIDAPAPAWVTDFTGWIAERMAAGAVDDILHAVERAP
                     HGRDNHPTPDHILPLFVAMGAGGVPFKAERLHDSVTYGLLAMDAYAFGEE"
     gene            complement(1478..3571)
                     /locus_tag="VN034_14950"
     CDS             complement(1478..3571)
                     /locus_tag="VN034_14950"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWV61960.1"
                     /translation="MLRQYELVERVRAYDPDVDEALLNRAYVFTVQKHGTQKRASGDP
                     YFSHPVEVAGILTDLHLDSETIVTALLHDTLEDTLTTPEEIERLFGPDVGRLVDGVTK
                     LSKIEAQTENERAAENLRKFLLAMSDDIRVLLVKLADRLHNMRTLHFIKNPDKRRRIA
                     KETMDIYAPLAERIGMYEYMREMQLLAFRELEPEAYATITGRLAKLTAGGKDKVAAIT
                     RDFKELLAGAGIEAEVSGREKHPYSIWRKMQERHVSFEQVTDIIAFRIVTPTDADCYA
                     ALGLIHRKWKMVPGRFKDYISTPKRNGYKSLHTTIMHQQNMRIEIQLRSLGMHQQSEF
                     GLAAHWAYKQGGSTPDGQAGWIRDLIEILEQTHDPEEFLENTRIAMYQDRIFAFTPKG
                     SLHQLPKGATPVDFAYAVHTGLGDRTVGAKVNGRLVPLRTQLSNGDTVEILSSDKQTP
                     QPAWLGFAVTGKARAAIRRHVRSKEKVELAALGRTMYDEMVARLPNKVGDKARAAARE
                     RLKLEDDSALYVAIAKQRLTDNAVLEALVPGITAEMKTKPGKLVQGAAVSIAGLTPGV
                     AFQLADCCHPVPGDRIVGLSRPGEGIEVHVIDCPSLADGIDADWIDLRWQEDSEGGNA
                     RLCIVILNEPGTLAEMSGILAANMANITNLRLSNREGGFHTYDVVVEVRDVQHVMRIL
                     SALRASDSVVQAERL"
     gene            3751..>4502
                     /locus_tag="VN034_14955"
     CDS             3751..>4502
                     /locus_tag="VN034_14955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWV61961.1"
                     /translation="MPVHNGARWLAEAIESVLAQDFRDFELILVDDASRDASPAIMAE
                     AAARDPRVRLLRLDTNVGLPAALNHGFAAARGELHSWTSDDNLLRPQMLARLVAKLDA
                     QPGADIVHADFILIDDAGREIGHMRVGPVERLLHGNNVGACFLYRARVTEALGGYDTA
                     LFGVEDYDFWLRAARHFTFATLHEDLYLYRKHGGSLTSQRAEHIQALTAEIVERAMPD
                     ALPVRSRSEILLGLALRSQHRWRFDLVRRAFRA"
BASE COUNT          702 a         1492 c         1489 g          819 t
ORIGIN      
        1 gctttattat tcgagctatg cgcatatcga gcagatggcc gacgccgtcg ccgagggcgc
       61 gcgcgccgcg ggggccgaag tcgacatccg ccgcgtcgcc gaaaccgcgc ccgaagcggt
      121 ggtgaaggcg gcgggcttca agaccgacac cgcgcatccg ctgctcagcg accccaatga
      181 cctcgtgaag tacgacgcgg tcgtcgtcgg caccccgacg cgcttcggcc gcatggcgag
      241 ccagatggcg agcttctggg acacggccgg cggcgtctgg gcgcagggca agctcaacgg
      301 caaggtcggc gccgccttca cctcgaccgc gtcgcagcat ggcgggcagg aaacgacgct
      361 cttctcggtc ctcaccaacc tcctccactt cggtatgacg atcgtcgggc tcgattatgg
      421 ctatgcgggc cagatgggcg tcgatgaggt caagggcggc gccccctatg gcgcgacgac
      481 gatcgccgat ggcgacggat cgcgccagcc gagcgaagcc gaccttggcg gcgcccgcta
      541 tctcggcgag cgtgtcgcga agaccgcagc gaagctcgcg gcctgacccg atgcgccgtc
      601 tcccgagcct tttcctttcg cacggctcgc cgatgatggc gctcgagccc agcccggcgc
      661 gggattttct cgccgggctg ggcgtccgat tgccgcgccc gcgcgcgatc ctcttcgtat
      721 cggcgcacca tgacgcggcg tatcagggcg gccgcgcgac ggtgaccgcg tcacccgcgc
      781 cgccgacgat ccacgatttc ggcggcttcc ccgacgaatt gttcgcgatg cgctaccccg
      841 cgccgggcga tccggcgctc gcggatcggg tcgtagagct gctcgcgggc cacggtcttg
      901 acatcgcggc cgatcccgaa cgcgggctcg accatggcgc atgggtgccg ctgtcgctga
      961 tctaccccct ggccgacatt cccgtcgtcc agctctcgat cgcctcgaac gcttcacccg
     1021 aatggcatca tgcgctcggc cgggcgctcg ctcccttgcg cgacgagggc gtgctcatcg
     1081 ccggatcggg cagcatcacg cataacctgc gcgcgctctt ctccgcccga ctgccgatcg
     1141 acgcacccgc gccggcgtgg gtcaccgatt tcactggctg gatcgccgaa cgcatggcgg
     1201 cgggcgcggt cgacgacatt ctgcacgcgg tcgagcgcgc gccgcacggc cgcgacaatc
     1261 atccgacgcc cgatcatatc ctgccgctct tcgtcgcgat gggcgcgggc ggcgttccgt
     1321 tcaaggccga acggctgcac gacagcgtaa cctacggcct gctcgcgatg gacgcttacg
     1381 ccttcggcga ggaataaggg ctccgcgcca agacgcgaat gatcgcttcg accggctagc
     1441 tgccgataga gatgtctgcc ccggcgaaac cgaccgtcta aagccgttcc gcctgaacca
     1501 cgctgtccga cgcgcgcagc gccgacagga tgcgcatcac atgctgaacg tcgcgcacct
     1561 cgacgacgac gtcataggtg tggaagccgc cctcgcggtt cgacagccgc aaattggtga
     1621 tgttcgccat attggcggcg aggatgcccg acatttcagc gagcgtgccg ggctcgttga
     1681 ggatcacgat gcagagccgc gcgttaccgc cctcgctatc ctcctgccag cgcaggtcga
     1741 tccagtcggc atcgatcccg tcggcgaggc tcgggcagtc gatgacatgg acctcgatcc
     1801 cttcgccggg gcgcgacagg ccgacgatgc ggtcgccggg caccgggtga cagcagtcgg
     1861 cgagctgaaa ggcgacgccg ggagtcaggc cggcgatcga caccgccgcg ccctgcacca
     1921 gcttgccggg cttggtcttc atctcggcgg tgatgccggg gacgagcgct tcgagcaccg
     1981 cattgtcggt gagcctttgc ttggcgatcg cgacatagag cgcgctatcg tcctcgagct
     2041 tcaatcgctc gcgtgccgcg gcgcgcgcct tgtcgccgac cttgttcggc agccgcgcga
     2101 ccatctcgtc gtacatggtg cggcccaatg cggcgagctc gaccttttcc ttcgagcgga
     2161 catggcggcg gatcgccgcg cgcgccttgc cggtgaccgc gaagccgagc cacgcgggct
     2221 gcggcgtctg cttgtccgac gacaggatct cgacggtgtc gccgttcgaa agctgggtgc
     2281 gcagcgggac gagccgcccg tttaccttgg cgcccacggt gcggtcgccg agcccggtgt
     2341 ggacggcata ggcgaaatca accggggtcg cgcccttggg tagctgatgc aggctccctt
     2401 tcggggtgaa ggcgaagatg cggtcctgat acatcgcgat gcgcgtgttt tcgaggaact
     2461 cctcgggatc gtgcgtctgc tcgaggattt cgatgaggtc gcggatccat ccggcctgcc
     2521 cgtcgggggt gctgccgccc tgcttgtagg cccagtgcgc ggcgagcccg aattcggact
     2581 gctgatgcat gccgagactg cggagctgga tttcgatccg catattctgc tggtgcatga
     2641 tcgtcgtgtg cagcgattta tagccgttgc gcttcggggt cgagatataa tccttgaagc
     2701 gcccggggac catcttccac ttgcggtgga tcaggcccag cgcggcatag cagtcggcgt
     2761 cggtcggggt gacgatgcga aaggcgatga tgtcggtcac ctgttcgaag ctgacgtggc
     2821 gttcctgcat cttgcgccag atcgaatagg gatgcttctc gcgccccgac acctcggcct
     2881 cgatccccgc gccggcgagc agttccttga aatcacgggt gatcgcggcg accttgtcct
     2941 tgccgcccgc ggtcagcttg gcgagccgcc cggtgatcgt cgcataggcc tcgggctcga
     3001 gctcgcggaa ggcgagcagc tgcatctcgc gcatatattc atacatgccg atccgctcgg
     3061 cgagcggggc atagatgtcc atcgtctcct tggcgatgcg gcggcgcttg tcgggattct
     3121 tgatgaaatg cagcgtgcgc atattgtgca gccggtcggc gagtttgacg agcagcacgc
     3181 ggatatcgtc cgacatggcg agcaggaatt tgcgcagatt ttccgccgcg cgctcatttt
     3241 cggtctgcgc ctcgatcttc gaaagcttcg tcaccccgtc gaccagccgg ccgacgtcag
     3301 ggccgaacag ccgctcgatt tcctcggggg tggtcagcgt atcctcgagc gtgtcgtgga
     3361 gcagcgcggt gacgatcgtc tcgctgtcga gatgaaggtc ggtcaggatg cccgcgacct
     3421 cgaccggatg gctgaaatag gggtcgcccg acgcgcgctt ctgggtgccg tgcttctgga
     3481 cggtaaacac ataggcgcgg ttgagcagcg cctcatcgac gtcgggatca taagcgcgca
     3541 cgcgctcgac aagttcatat tgacgcagca tcgatcccaa tgtcgttcat gttgcatata
     3601 ttgcaatgcg aaatttgatt gcgcccggcg ccaatgcgtt agcaactttg gtaaagcccg
     3661 ttggaagcat cgttcgtcgc gggtaggacg ttgggcgaga tggggaccca aactctcgat
     3721 tcgaccgcgc cgccgcgggt gtcgatagtg atgcccgtgc acaatggtgc gcgctggctc
     3781 gccgaggcga tcgagtcggt tctggcgcag gatttccgcg atttcgaact gatcctcgtc
     3841 gacgatgcgt cgcgcgacgc ctcgcccgcg atcatggcgg aagccgcggc gcgcgacccg
     3901 cgcgtgcggc tgttgcggct cgatacgaac gtcggcctgc ccgcggcgct caatcacggc
     3961 tttgccgcgg cgcgcggcga actgcacagc tggacgtcgg acgacaatct gctccggccc
     4021 caaatgctgg ctcgcctcgt cgcgaagctc gacgcacaac ccggtgccga tatcgtccat
     4081 gccgatttca tcctgatcga cgatgcgggc agggaaatcg ggcacatgcg ggtcggccct
     4141 gtcgagcggt tgctccacgg caacaatgtc ggcgcctgtt tcctctatcg cgcgcgcgtg
     4201 accgaggcgc tcggcggcta tgacaccgcg ctgttcggcg tcgaggatta tgatttctgg
     4261 ctccgcgcgg cgcggcactt caccttcgcc acgctgcacg aggatctcta tctctatcgc
     4321 aagcatggcg gcagcctgac gagccagcgc gccgagcata tccaggcgct gaccgccgag
     4381 atcgtcgaac gcgcgatgcc cgacgcgctt cccgtgcgca gccgcagcga aatcctgctc
     4441 gggctcgcgc tgcgcagcca gcaccgttgg cggttcgacc tggtgcgccg cgcgttccgc
     4501 gc
//
LOCUS       DATMSU010000561         4970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_170281,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000561 DATMSU010000000
VERSION     DATMSU010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170281"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VN034_14960"
     CDS             <1..320
                     /locus_tag="VN034_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWV61962.1"
                     /translation="RCLRSPTPARSHRRIITARRHAAERLVPLGIPASGTQILPVIIG
                     DNDRTMRIAGQLQGAGFDIRGIRPPTVAEGTARLRIAITLNVDEDNIDDMADALAQAM
                     AAA"
     gene            317..925
                     /gene="bioD"
                     /locus_tag="VN034_14965"
     CDS             317..925
                     /gene="bioD"
                     /locus_tag="VN034_14965"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWV61963.1"
                     /translation="MTRFVVTGTDTGIGKTIFSAALAQATGAHYWKPIQSGLEEETDS
                     EAVARLAGVPIRPEAYRLVTPASPHLAAEIDGVNIDVETLTPPPGDLIVEGAGGALVP
                     VTRTTLYADLFAKWQVPVIVCARTSLGTINHSLLTIEALKSRDVPLHGLAFLGDAVED
                     SEAIIAEISGVRRLGRLPIIDPLNSETLADAFKANFDLADFR"
     gene            complement(1021..3456)
                     /locus_tag="VN034_14970"
     CDS             complement(1021..3456)
                     /locus_tag="VN034_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61964.1"
                     /translation="MQGAAPPGVIGAHHRPDDIVVTAARRGEAKISAESEFSEDEIAA
                     QGADSIEDLLTHLAPFIDGSGEDPVILINGKPAGFDLSILSYPAEALDRLAVLKPEAA
                     AHYGEPAGKRVVNLVLKKNFSMLNTDASADFATAGGQYGGTLSVRRTAISGDTRWNAQ
                     ARAGADSAFRKTGRDIPPRDGVFDSVGFISSLDGGEIDPALSLAAGQLVTVAAIPRGA
                     LIGVPDLNDFVATASALHPVDPNRFETLQSSRRTAALSIGVTRPLGDFSVALNLNANR
                     TSSEGERGLPMASVVIPAGHPWSPFAEDVMLTRPFAGERALRTDNDSTSLGASLTLNG
                     SIGGWQTSLAASYSRNWAENFFENEVDVARVQQLIDDLDPDFNPYGAWDEGLMIAARN
                     RTNSENLSARLNVRKTVVELPAGPLVWNLTANSGRNSTQSRQRDAAGNLTAASSLARS
                     QSSGRMSISVPISRVGVAGSDWLGDLIVDLSASAQTMTNSRPQKGVAGSINWSPWPVV
                     QLRGAIDFAENAPSFEQLDAPIVTTVNRLFDYLRQEIAEPIWITGGNPDLDRGRRQSL
                     MLSATVRPFGDQVLTINLGYRQSVVKGGVTGFPELTPAIEAAFPERVTRDAEGRLVAV
                     DARAINLARQTDSSLSTGIALRLPGRRPGRGALAADPLQFSISFNHNMRLKSELLARP
                     GMLAIDQLRAGGQSRHSLSFQASVGKRGIGANLGGSWSSSGFLRGGDEIFIFKPPLTL
                     NVSAFIEPDRLFKVPGNKGVLNNLKVSVDIDDLLNGYRRVTREEGTVPAGYSRNEIDP
                     LGRTVRLTLGKKF"
     gene            complement(4325..4828)
                     /locus_tag="VN034_14975"
     CDS             complement(4325..4828)
                     /locus_tag="VN034_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWV61965.1"
                     /translation="MKKLAMICIAALASASTVAPAFAQHITPASTPFTFTGPAVLNKP
                     GFPTSSCTLTLTGITNPTLGLPAPHVNHTDGASVTGGTNTGGGACPAITVGPADISYN
                     STTSTWQIEGLKVYVGPNLVCDDAGPIAFGLSTSGGVSTAVFNSTVDPDCNVMAELTS
                     TDVQAVP"
BASE COUNT          954 a         1604 c         1513 g          899 t
ORIGIN      
        1 ggcgttgctt gcgctcgccg acgcccgccc gctcacacag acgcatcatc actgcccgcc
       61 gccacgccgc cgagcgcctc gtgccgctcg gcatccccgc cagcggcacc cagatcctgc
      121 ccgtgatcat cggcgacaac gaccgcacaa tgcggatcgc gggccaattg cagggcgccg
      181 gcttcgacat tcgcggcatc cgcccgccga cggtagccga gggtaccgcg cgcctccgca
      241 tcgcgatcac gctcaatgtt gacgaagata acatcgacga tatggccgac gcgctggcgc
      301 aggcgatggc cgcggcatga cgcgcttcgt cgtcaccggc accgacaccg gcatcggtaa
      361 aaccatcttt tccgccgctt tggcacaggc gaccggcgcg cattattgga aaccgatcca
      421 gtcggggctc gaagaggaaa ccgacagcga ggcggtcgcg cgcctcgccg gcgtgccgat
      481 ccgccccgaa gcctaccgcc tcgtcacccc cgcctcgccg catttggccg ccgaaattga
      541 cggcgttaac atcgacgtcg aaacgctgac cccgcccccc ggcgacctga tcgtcgaggg
      601 cgccggcggc gcgctcgtcc ccgtcacgcg caccacgctc tacgccgacc tgttcgcgaa
      661 atggcaggtt ccggtgatcg tctgcgcgcg caccagcctc ggcacgatca accacagcct
      721 cctgaccatc gaggcgctca aatcacgcga cgtcccactc cacggcctgg ccttcctcgg
      781 cgacgcggtc gaggacagcg aagcgatcat cgcggaaatc tcgggcgtcc gccgcctggg
      841 ccgcctgcct atcatcgatc cgctgaacag cgaaacactg gccgacgcat tcaaagcgaa
      901 cttcgacctc gctgactttc gatagatatt cgtcatcccg gcgaaagcga ccaccgacgt
      961 tgctctccgg cagggacgtg ggcggatcgc gcgggcgtgt ccaaaagcaa aggcgcagca
     1021 tcagaatttc ttgcctagtg tcagccggac ggtacgcccg agcgggtcga tctcgttgcg
     1081 gctgtatccc gcaggcaccg tcccttcttc gcgcgtcacg cggcggtagc cgttgagaag
     1141 gtcgtcgata tccaccgaca ccttaaggtt gttcagcacg cctttattgc ctggaacctt
     1201 gaacagacgg tcgggctcga tgaaggctga gacattgagc gtcagcggcg gcttgaagat
     1261 gaagatttca tcgccccctc gcaagaaccc cgaactgctc caactaccgc ccagatttgc
     1321 gccgatcccc cttttcccga ccgaagcttg aaacgacagg ctgtggcgcg attgcccgcc
     1381 cgcgcgaagc tgatcaatcg cgagcatgcc ggggcgggcc agcaattcgc ttttcaggcg
     1441 catgttgtga ttgaacgaaa tcgagaattg cagcggatcg gcagccaacg ctccgcgccc
     1501 aggccgccgt ccgggaagac gaagcgcaat gccggtagac agcgaactgt cggtttggcg
     1561 cgcaaggttg atcgcgcggg catcgaccgc gaccagccgc ccctccgcat cgcgcgtcac
     1621 ccgctcggga aaggccgcct cgatggcggg tgtcagctcg ggaaacccgg tcacgccgcc
     1681 cttcaccacc gactggcgat agccgaggtt gatggtcagc acctgatcgc cgaacggccg
     1741 caccgtcgcg gacagcatca ggctttgccg gcggccacgg tccagatcgg gattgccgcc
     1801 ggtgatccaa attggctccg cgatctcctg gcgcaaatag tcgaagaggc ggtttaccgt
     1861 cgttacgatt ggcgcatcga gctgctcgaa cgagggcgcg ttttcggcga agtcgatggc
     1921 cccgcgaagc tggacgaccg gccatggcga ccagttgatg ctaccagcga cccccttttg
     1981 cggccggctg ttcgtcatcg tctgcgcgct ggcggacagg tcgacgatca ggtcgcccag
     2041 ccaatcgctc ccggccacgc cgacgcgcga gatcggcacg ctgatcgaca tccggccgct
     2101 tgactggctg cgcgccaggc tgcttgccgc cgtcaaattc cccgccgcgt ccctttgccg
     2161 gctttgggtg ctgttgcggc cgctgttggc ggtaaggttc cagacgagcg gcccggcagg
     2221 caactctacg acagtctttc gcacgttgag ccgggcgctg agattttcgc tgttcgtccg
     2281 attgcgagcg gcgatcatca agccttcgtc ccatgcgcca taggggttga agtccggatc
     2341 gaggtcgtcg atcaactgct gaacgcgcgc aacgtcgacc tcattttcga agaaattctc
     2401 ggcccaattg cgcgaatagc tcgccgccag actcgtctgc cagccgccga tactgccatt
     2461 gagcgtcagc gaagcgccga gcgaggtcga gtcattgtcg gtgcgcagcg cacgctcacc
     2521 cgcaaaaggc cgggtcaaca tgacatcctc cgcaaaaggc gaccaaggat ggccggcggg
     2581 gatgacgacc gacgccatcg gcaggccccg ctcgccttcg ctgctggtcc ggttcgcatt
     2641 gaggttcagt gcgacggaga aatcaccgag agggcgtgtc acgccgatgc tgagcgcggc
     2701 ggtgcgccgg gacgattgta gcgtttcaaa gcgattggga tcgacggggt ggagcgcgct
     2761 cgcggtcgcc acgaaatcgt tgagatcggg aaccccgatc agcgcgccgc gcgggatggc
     2821 ggccacggtt accagctgtc cggcggcaag gctgagcgcg gggtcgatct ctccgccgtc
     2881 gaggctggag atgaaaccga cgctgtcgaa cacgccgtcg cgtggcggga tatcgcgccc
     2941 cgtctttcga aacgcgctgt ccgcgccggc ccgagcctgc gcgttccagc gtgtgtcgcc
     3001 gctgatcgcc gtccgccgga ccgaaagtgt gccgccatat tggccgccgg cggtcgcgaa
     3061 atccgcgctc gcgtcggtat tcagcatcga aaaattcttc ttgagcacca gattgacgac
     3121 gcgcttgccc gccggctcac cataatgcgc tgccgcttcg ggcttgagca cggccagccg
     3181 gtcgagcgcc tcggcaggat aggaaaggat cgaaaggtcg aatccagcgg gcttaccgtt
     3241 gatcagaatg acaggatctt cgccgctacc gtcgataaag ggagcgagat gcgtcagcag
     3301 gtcctcaata ctgtcggcgc cctgcgccgc gatctcatcc tcgctgaact cgctttctgc
     3361 ggaaatcttc gcctcgccgc ggcgagccgc agtgacgacg atatcgtcgg gacggtggtg
     3421 cgcgccgata acgcccggcg gggcggctcc ttgcaaagcc gtccccgccg cgacgtcccc
     3481 cttatccgtc tgcgcatgag ccaggacggc gctaccgacc aggcccagca ccaatgggga
     3541 tgaaactgct ttcagtaccg acacatgcca agccgcctct aaatgcttag gaaacgacgg
     3601 taaaatctgc gctcaaactg gcctccacaa aacaagttcc tatcgtggcg ccaatctcga
     3661 tcatcgacgt cgtcgcgctc gtattcgtga ttgtaaacgg aactgttgct ccgcccggcg
     3721 ggccgcaaat gccgagttcg atggcgttaa agactccggt gccgcccccc atcccgatgc
     3781 ttgaaatgct gaagctcggg cccgacgcgg cattaatcga gaggaggggg cacacggccg
     3841 cccccgtaac gggtccggac gtaatcgtgc cgcccaacgc atgcccagcg tgggcgcccc
     3901 ctatatcggc gccggtggtg cccatcattg tgagcgtaca ggtgagagac ccagacccga
     3961 gcggagtgat cactacgggg cctccagcgg taacggccgt gttcggcggc gaaacatgtt
     4021 gcgctagggc ggggttgggc acggctgcta tcaggagcgc cccgacaaga ggcaaagtcg
     4081 atcttttcat ttctcttccc tgttcatcga ctggacgatc acgttcgcac cggcgacgcg
     4141 ccaatgctgc aatattgcca gacctgcgcg aaggccctag gaggagcgtt ccggatggaa
     4201 ccacggcaac tggttcaaga tcgctcccgt ttgcagggac ggtatccggt tgggagcgga
     4261 tgcttcgatc cttctatgat ggatcgaagc atccgcggta tcacgcttca atatttccgg
     4321 ctcgtcaggg gactgcttga acgtccgtgg acgtcaattc ggccatgacg ttacaatcgg
     4381 gatcgacagt cgaattgaat actgcggtgc ttactccgcc cgaagtcgac aagccgaatg
     4441 caatcggtcc agcatcatcg cagacgagat tgggccccac ataaactttg agaccttcaa
     4501 tctgccaagt cgaggtagta gaattgtagc taatgtctgc tggaccaacc gtgattgcag
     4561 ggcaagcacc accgccagta ttggtaccgc cggtaaccga tgcgccatcc gtgtgattta
     4621 catggggcgc cggcaagccg agcgtcgggt tcgtaatgcc cgtcagggta agggtgcagc
     4681 tcgatgtcgg aaaaccaggc ttattgagca cagcaggacc cgtaaaggta aacggcgtgc
     4741 tggccggggt aatatgctgg gcaaaggccg gcgcaacggt gctggcggag gccagcgccg
     4801 cgatacagat catagcaagc tttttcatca tgcttctcct ctcactatcg gccagttcgg
     4861 ccgtttttaa atgatcgcga atttaccatt tcgcgatcaa taccactccg atgcggagcg
     4921 atggggggat gggagagttt ctaattatgc agtatatcaa ctaaacatat
//
LOCUS       DATMSU010000562         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_170370,
            whole genome shotgun sequence.
ACCESSION   DATMSU010000562 DATMSU010000000
VERSION     DATMSU010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170370"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..854)
                     /locus_tag="VN034_14980"
     CDS             complement(123..854)
                     /locus_tag="VN034_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWV61966.1"
                     /translation="MIQTLRTLIVDDEPLAIERLQILTGQQDGISLVGTASDGASALR
                     MVDALAPDLVLCDIAMPDLNGLEVAAAIERMDNPPAVVFVTAFDRYAVAAFDVAAVDY
                     LLKPVSPDRLARALSRVREWRATERTPAQKSKWINEFWVQNRGEMLRIDAGQVDLIEA
                     ERDYMRLHVGGRSWLIHQTIKSLEARMNPDQFMRIHRSKMIRREGIVGLKHHGDGAWS
                     VDLGEGGVHRIGRTYLHDVKAIMHA"
     gene            complement(856..2028)
                     /locus_tag="VN034_14985"
     CDS             complement(856..2028)
                     /locus_tag="VN034_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWV61967.1"
                     /translation="MNVTNRKDALRDQRAKMLAAAPDWRASDARVNPRVAIGSIIAMW
                     VIYFLITTGLALLTGAPEQWSYAGRRAIVVVAGILCTFVLYQLLQRAQPQSFGARLAA
                     AMGAAVPLVVVYATINLLVFFYWFPAPDTAKIIEEVQSKFPVAWEMMLILDSSIRWYF
                     FFAVWAALYVAFGYANEMRAVERRANHFRMEAQTAQLRALHYQVNPHFLFNTLNSLST
                     LVLKGSKSEAETMIMNLASFLRSSLAVDPEQLVSLDEEIALQQLYLDIEQTRFPDRLQ
                     VEVAMPDELKYACVPVLILQPIIENAIKYGVAPSKGKIAIRLSASAEYGLLILRIEND
                     IDPRAPIPASGTGLGLGNVRERLLTRYGPTAGCEWGPSDGGGFVVSLWLPLAREAC"
     gene            complement(2277..>3458)
                     /locus_tag="VN034_14990"
     CDS             complement(2277..>3458)
                     /locus_tag="VN034_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HWV61968.1"
                     /translation="FGAFFGFGMAATVLIGQSMGRGDINAARRATGGVIGLALLFSVV
                     IAVVGWFASDRILGWLETPPEAFALAHDYLRVTFLAVPASMLSVTLMMASRGAGDAMT
                     PLRFMILAVVLDIVFNPVLILGLGPFPRLGIAGSALATAAAGTISLAGMIGWFYAKNH
                     VLRLRGRELSYLYPKWSELRFIIGRGLPMGAQMLVISGAGLVMVGLVNREGLVTAAAY
                     GATLQLWNYIQMPALAVGAAVSSMAAQNIGAGRWDRVASVTNSGIMINLAMTGVLIVL
                     LLIFDRAALALFLGSESPAIEVSRNIQLIATWTFLPFGTTIVLISTLRANGSVVPPLV
                     ILFLSMFPIRLGIYYFGYPAVGSDILWWSFPLSSLASLAMAWAIYRRGDWRRTLPQPV
                     A"
BASE COUNT          642 a         1055 c         1204 g          557 t
ORIGIN      
        1 gagccccctc cgtcagccgc tgcgcggctg ccacctcccc acaagtgggg aggatctaac
       61 gtccactccc caccccaaaa accgacattg tccggcgagc tcgcggcatg gccgcctttt
      121 cgtcaggcgt gcatgatcgc ctttacgtcg tgcagatagg tacggccgat gcggtggacg
      181 ccgccttcgc ccaggtcgac gctccacgcg ccgtcgccat gatgtttcaa tccgacgatg
      241 ccttcgcggc ggatcatctt cgaccggtgg atgcgcatga actggtcggg gttcatgcgc
      301 gcttccaatg acttgatcgt ctggtggatg agccagctgc ggccgccgac gtgcaggcgc
      361 atatagtcgc gctcggcctc gatcaggtcg acctggcccg cgtcgatccg cagcatctcg
      421 ccgcggttct gcacccagaa ttcgttgatc cacttgctct tttgcgcggg cgtacgttcg
      481 gtcgcgcgcc attcgcggac gcgcgacagc gcgcgcgcga ggcgatcggg ggatacgggc
      541 ttgagcagat agtcgaccgc ggcgacgtcg aaggccgcga cggcatagcg atcgaaggcg
      601 gtgacgaaga cgaccgcggg cggattgtcc attcgctcga tcgccgcggc gacctccagc
      661 ccgttgagat cgggcatcgc gatgtcgcac agcacgaggt cgggcgcgag cgcgtcgacc
      721 atgcgcagcg ccgaggcgcc gtcgctggcg gtgccgacga gcgagatgcc gtcctgctgc
      781 ccggtcaata tctggagccg ctcgatcgcg agcggttcgt cgtcgacgat cagggtgcga
      841 agtgtctgga tcatttcagc atgcctcccg tgccaacggc aaccagagtg aaacgacgaa
      901 gccgccgccg tccgacggac cccattcgca gcccgccgtg gggccatagc gcgtgagcag
      961 ccgttcgcgg acattgccga ggccgagacc ggtgcccgac gccggaatcg gcgctctggg
     1021 atcgatgtcg ttttcgatgc gcaggataag cagcccatat tcggcgctgg cggagaggcg
     1081 gatcgcgatt ttccccttgc tgggcgcgac gccatatttg atcgcatttt cgatgatcgg
     1141 ttgcaggatc aagaccggca cgcaggcata tttcagctcg tcgggcatcg ccacttcgac
     1201 ctggagccgg tcgggaaagc gcgtctgctc gatatcgaga tagagctgct ggagcgcgat
     1261 ttcctcgtcg aggctgacga gctgttcggg atcgacggca aggctcgaac ggaggaagga
     1321 cgcgaggttc atgatcatcg tttcggcctc gctcttcgac cccttgagca cgagcgtcga
     1381 cagcgagttg agcgtattga acaggaagtg cggattgacc tggtaatgga gcgcgcggag
     1441 ctgcgcggtc tgcgcctcca tccggaaatg attggcgcgc cgttcgaccg cgcgcatttc
     1501 attggcatag ccgaaggcga cgtaaagcgc ggcccagacg gcgaagaaga aataccagcg
     1561 gatcgagctg tcgaggatca gcatcatttc ccatgcgacg ggaaatttcg attgcacttc
     1621 ctcgatgatc tttgcggtgt cgggcgcggg aaaccagtag aagaagacga gcaggttgat
     1681 tgtcgcataa acgacgacga gcgggaccgc ggcgcccatc gcggcggcga ggcgcgcgcc
     1741 aaagctttgc ggctgggcgc gctggagcag ctgatagagg acgaaggtgc acaatatgcc
     1801 ggcgacgacg acgatcgcgc ggcggccggc ataggaccat tgttccggcg cgcccgtgag
     1861 cagggcgagg ccggtcgtga tcaggaaata gatcacccac atcgcgatga tcgatccgat
     1921 cgcgacgcgc gggttgacgc gggcgtcgga agcgcgccag tcgggcgccg ccgccagcat
     1981 tttcgcgcgc tggtcgcgca gcgcgtcttt gcggtttgtc acattcattg acgttgcttt
     2041 agcggcaaaa gccggtcgat ggctagcggg cgccgggccg ccagtcgaag ctgtgtgggc
     2101 cttggtcgaa aggggttctg gagcgcgatc attctttcct gaatcgtcat cccggcgaag
     2161 ggtgttcaga ggcacgtgac tctgaaacac gatctcgccc gtgcgtccgg gccgaggcgg
     2221 cgagatcccg gccttcgccg ggatgacgag atagggtagg ctaaaccggt cgcgcttcag
     2281 gccaccggtt gcggcagcgt gcggcgccag tcgccgcgcc ggtagatcgc ccacgccatc
     2341 gctagcgagg cgagcgacga caaaggaaag ctccaccaca atatgtcgct gcccaccgcc
     2401 ggataaccga aataatagat gccgaggcgg atcgggaaca tcgacaggaa aaggatgacg
     2461 agcggcggga cgaccgagcc gttggcgcgc aaggtgctga tcagcacgat cgtcgtgccg
     2521 aagggcagga aggtccaggt cgcgatcagt tgaatattgc gcgatacctc gatcgccggg
     2581 ctctcgctgc cgaggaagag cgcgagcgcg gcgcggtcga agatcaggag caggacgatc
     2641 agcaccccgg tcatcgcgag gttgatcatg atgccgctgt tcgtcaccga cgcgacgcgg
     2701 tcccagcggc ccgcgccgat attctgcgcc gccatcgagc tgaccgcggc gccgaccgcg
     2761 agcgcgggca tctggatata gttccagagc tggagcgtcg cgccataggc ggcggcggtg
     2821 acgagcccct cgcggttgac gagcccgacc atgacgagcc cggcgcccga gatgacgagc
     2881 atttgcgcgc ccatcggcag gccgcggccg atgatgaaac gcagctccga ccatttgggg
     2941 tagagatagg atagttcgcg gccgcgcagg cggaggacgt ggttcttcgc gtagaaccag
     3001 ccgatcatgc ccgcgaggct gatcgtgccg gcggcggcgg tcgcgagcgc gctgcccgcg
     3061 atgccgaggc gcgggaaggg gccgaggccg aggatcagca ccgggttgaa gacgatgtcg
     3121 agcacgaccg cgaggatcat gaagcggagc ggcgtcatcg cgtcgcccgc gccgcgcgac
     3181 gccatcatca gcgtgacgct gagcatcgag gcggggacgg cgagaaaggt gacgcgcaga
     3241 taatcatgcg cgagcgcgaa ggcttcgggc ggggtttcga gccagccgag gatgcggtcc
     3301 gacgcgaacc agccgacgac cgcgatgacg accgagaaga ggagcgcgag gccgatgacg
     3361 ccgccggtcg cgcggcgcgc ggcgttgatg tcgccgcggc ccatcgactg gccgatgagc
     3421 accgtcgccg ccatgccgaa gccgaagaag gcgccgaa
//
LOCUS       DATMSU010000563         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingopyxis sp. isolate SMAG_U12994 k141_18921, whole
            genome shotgun sequence.
ACCESSION   DATMSU010000563 DATMSU010000000
VERSION     DATMSU010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943595
            Sequence Read Archive: SRR7448274
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingopyxis sp. (soil metagenome)
  ORGANISM  Sphingopyxis sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingopyxidaceae; Sphingopyxis.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 20:07:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,999
            CDSs (total)                      :: 2,974
            Genes (coding)                    :: 2,963
            CDSs (with protein)               :: 2,963
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 1 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Sphingopyxis sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18921"
                     /isolate="SMAG_U12994"
                     /isolation_source="soil"
                     /db_xref="taxon:1908224"
                     /environmental_sample
                     /geo_loc_name="China: Jiaozhou"
                     /lat_lon="34.2487 N 119.8167 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..3120
                     /locus_tag="VN034_14995"
     CDS             328..3120
                     /locus_tag="VN034_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWV61969.1"
                     /translation="MAINAMRSARALTRLACGASLAALALSPAHAQDAAGATASEDEI
                     VVTGFRASLDKALDQKRDSIAAVDVIVAEDIAKFPDQNLAESLQRIPGISIQRDAGEG
                     RAITVRGLGAQFTRVRLNGMETIATSTDGAAANRDRAFDFNVFASELFTSLVVHKTAS
                     ASLDEGSLGAVVDLNTGNPLGGKEGFTAVASVQGRYNDLTKDVDPRLAGLIAWTNADR
                     TFGVSASVAWADYKTHELGNNSVRWAQAPFRSVDGVTCLAGSDFVANPSDACIEVAEA
                     FHPRIPRYGLVSHDRERLGATASIQWEPSENTKISIDGLYSTFKETRDEFWGEVLLRS
                     EEDAIDVSNYTIDGDNNLIAADLDNVYVRTERYSRQSKTEFYQISGRLEQRLTDTLAV
                     NLLGGFSKSQADIPVETTLAFDDTDATGYRYDYTNMKYPLLTFGAGIDDPSSFVLAEF
                     RDRPSFLTNKFKTFAADFDWDVADRFKMRGGGFYRQFDFDTVGYRRDSSYCSAFTCAP
                     GTGGLPVTSDIAELFELGDAGQPSGNTNAWIVPDLDAGTALIDLYGRPAVLQQGEQRA
                     VTEKTYGGWFMAEFETDLFGMRMTGNAGVRYAKTDQSSSGFTSGTFVTVDRTYDDWLP
                     SFNVNLHPSETIILRGAIAKVITRPTLGSLTPGGSVDQFNFRISSGNPFLEPYRATTF
                     DLAAEWYFAPGAIASVALFAKDIESFPLSDSFQGTYASSGLPLSLLTPGTPAYEAIVE
                     GDNPGREFEFRTTVNGPGASLKGVELSLQLPFSVFSDSLRHFGVLGNTTFVESDVDYA
                     IRGPLVYDPDDNQLEAPVASTFTQPLLGLAKRAWNATVYYDDGKFSVRTSAAWRSGYN
                     DSTSGNNNVFEGYGSSFNVDASIRYALTDNIELSIEGTNLTDDYRYRFTDLEANRNYE
                     NNHYGRTFLFGARFKI"
BASE COUNT          590 a         1096 c          935 g          579 t
ORIGIN      
        1 gccgacgtcg gtacggccgg catcgcaagc gccagaaaaa gcgcggttat ccaaggtttc
       61 cggcgcatct ctctctccct attctttggg tattgacacg gctacccata tccttcaatc
      121 ttcctgacac cggttacccc aatcggtcaa cgagaaaata tttccaaggg agagggtctg
      181 atccggttcg tcgcacttgg cggcggcctg tcatcactct ctgaaaaatc gacggatgaa
      241 acggtcagcc aaatcgtttc gtccagcaaa gtaaccggtg tcatttcttg cgcaggcggg
      301 ggcaacgggc gataagggag gatgataatg gcaataaatg caatgcggag cgctcgcgct
      361 ttgacccggc tggcgtgcgg cgcgtcgctt gccgcgctgg cgctgtcgcc ggcccatgcg
      421 caggacgccg ccggggcgac cgcaagcgag gacgagatcg tcgtcaccgg cttccgcgcc
      481 tcgctcgaca aggcgctcga ccagaagcgc gactcgatcg ccgcggtcga cgtcatcgtc
      541 gccgaggaca tcgccaaatt ccccgaccag aatctcgccg aatcgcttca gcgcatcccg
      601 ggcatctcga tccagcgcga cgccggcgaa ggccgcgcga tcaccgtgcg cgggctcggc
      661 gcgcagttca cccgcgtccg cctcaacggg atggaaacga tcgccacctc gaccgacggc
      721 gctgcggcca accgcgaccg cgcgttcgac ttcaacgtct tcgcgtccga actcttcacc
      781 tcgctcgtcg tccacaagac ggcctcggcc tcgctcgacg aaggctcgtt gggcgcggtc
      841 gtcgatctca acaccggcaa tccgctcggc ggcaaggaag gcttcaccgc cgtcgcctcg
      901 gtgcaggggc gctacaacga cctgaccaag gacgtcgatc cgcgcctcgc gggcctcatc
      961 gcctggacca atgccgatcg gaccttcggc gtctccgcct cggtcgcctg ggccgactac
     1021 aagacgcacg agcttggcaa caacagcgtg cgctgggcgc aggcgccgtt ccgcagcgtc
     1081 gacggcgtca cctgccttgc gggctccgac ttcgtcgcca atccgtcgga cgcctgcatc
     1141 gaggtcgccg aagcctttca cccgcgcatc ccgcgctacg gcctcgtcag ccatgaccgc
     1201 gagcgcctcg gcgccaccgc gtcgatccag tgggagccga gcgagaacac caaaatctcg
     1261 atcgacggcc tctattcgac cttcaaggag acccgcgacg aattttgggg cgaggtgctg
     1321 ctgcgcagcg aagaggacgc gatcgacgtg tcgaattaca cgatcgacgg cgacaacaac
     1381 ctcatcgccg ccgacctcga caatgtctat gtccgcaccg aacgctattc gcgtcagagc
     1441 aagaccgaat tctaccagat ttcgggccgg ctcgaacagc gcctgaccga caccctcgcg
     1501 gtcaatcttc tcggcggctt ctcgaaatcg caggccgaca ttccggtcga aacgacgctc
     1561 gcgttcgatg acacggacgc gaccggctat cgttacgatt atacgaacat gaaatatccg
     1621 ctgctgacct tcggcgccgg catcgacgat ccgtcgtcct tcgtgctcgc cgagttccgc
     1681 gaccgtccgt ctttcctgac caacaagttc aagaccttcg cggccgattt cgactgggat
     1741 gtcgccgacc ggttcaagat gcgcggcggc ggcttctatc gccagttcga tttcgacacc
     1801 gtcggctatc gccgcgacag cagctattgc agcgccttca cctgcgcgcc gggaaccggc
     1861 ggactgccgg tcacctcgga catcgccgaa ctgttcgaac tcggcgacgc cgggcagccg
     1921 tcgggcaaca ccaacgcctg gatcgttccc gatctcgacg cgggcaccgc gctgatcgat
     1981 ctttatggcc gccccgcggt gctccagcag ggcgaacagc gcgccgttac cgaaaaaacc
     2041 tacggcggct ggttcatggc ggagttcgag accgacctct tcggcatgcg gatgacgggc
     2101 aacgcgggcg tccgctatgc caagaccgat cagtcgtcgt cgggtttcac cagcggcacc
     2161 ttcgtcacgg tcgaccggac ctatgacgac tggctgccct cgttcaacgt caacctgcat
     2221 ccgagcgaga ccatcatcct gcgcggcgcg atcgccaagg tgatcacgcg tccgacgctg
     2281 ggcagcctga ccccgggcgg ctcggtcgac cagttcaatt tccgcatcag ctcgggcaac
     2341 ccgttcctcg aaccctatcg cgcgacgacc ttcgacctcg ccgccgaatg gtatttcgcg
     2401 ccgggggcga tcgcgtcggt ggcgctgttt gccaaggata tcgaaagctt cccgctctcg
     2461 gacagcttcc agggcaccta tgcgtcgagc ggcctgccgc tctcgctcct gaccccggga
     2521 acgcccgcct atgaggcgat cgtcgagggc gacaatccgg gccgcgagtt cgagttccga
     2581 acgaccgtca acggccccgg tgccagcctg aagggcgtcg agctgtcgct gcagctgccc
     2641 ttctcggtct tttcggattc gctgcgccac ttcggcgtgc tcggcaacac gaccttcgtg
     2701 gagagcgacg tcgattacgc catccgcggt ccgctcgtct atgatccgga cgacaaccag
     2761 ctcgaggcgc cggtcgcgag caccttcacc cagccgctgc tcgggctcgc caagcgcgcg
     2821 tggaatgcca ccgtctatta tgacgacggc aaattttcgg tccgcacctc ggcggcgtgg
     2881 cgcagcggct acaacgacag caccagcggc aacaacaatg tgttcgaagg ctatggcagc
     2941 tcgttcaacg tcgatgcgtc gatccgctat gcgctgaccg acaatatcga attgtcgatc
     3001 gagggcacca acctgaccga cgattaccgc tatcgcttca ccgatctcga agcgaaccgc
     3061 aattacgaga ataatcatta tggccgcacc ttcctgttcg gtgcgcgctt caaaatctga
     3121 cacggaacga tttcgtcccg gtgaggcccg gccttcgctt gcgggggccg ggcctttctt
     3181 tttttgcgag gagagacaag
//
